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1

Cloning and characterization of a ?-galactosidase encoding region in Lactobacillus coryniformis CECT 5711  

Microsoft Academic Search

A chromosomal DNA fragment of 7.8 kb from Lactobacillus coryniformis CECT 5711 was cloned in Escherichia coli K-12 and was found to express a functional ?-galactosidase. Nucleotide sequence analysis showed that this fragment contained two partially overlapping genes, the lacL (1,881 bp) and the lacM (960 bp), that encode the subunits of a heterodimeric ?-galactosidase, with estimated molecular masses of 72,129 and 35,233 Da,

J. M. Corral; O. Bañuelos; J. L. Adrio; J. Velasco

2006-01-01

2

Cloning and characterization of a beta-galactosidase encoding region in Lactobacillus coryniformis CECT 5711.  

PubMed

A chromosomal DNA fragment of 7.8 kb from Lactobacillus coryniformis CECT 5711 was cloned in Escherichia coli K-12 and was found to express a functional beta-galactosidase. Nucleotide sequence analysis showed that this fragment contained two partially overlapping genes, the lacL (1,881 bp) and the lacM (960 bp), that encode the subunits of a heterodimeric beta-galactosidase, with estimated molecular masses of 72,129 and 35,233 Da, respectively. Other three incomplete open reading frames showing homology to another beta-galactosidase, an alpha-galactosidase, and a galactokinase, respectively, were also found. The L. coryniformis beta-galactosidase was overproduced in E. coli by using an isopropyl-beta-D: -thiogalactopyranoside (IPTG) expression system. Two new proteins with an estimated M (r) s of approximately 72,000 and 35,000 appeared upon induction with IPTG, and extracts of the recombinant E. coli strain showed beta-galactosidase activity. PMID:16820950

Corral, J M; Bañuelos, O; Adrio, J L; Velasco, J

2006-12-01

3

In vitro assessment of immunomodulating activity of the two Lactobacillus strains isolated from traditional fermented milk  

Microsoft Academic Search

The objective of this study was to evaluate the in vitro immunomodulating capacity of Lactobacillus\\u000a coryniformis subsp torquens T3L (L.\\u000a coryniformis T3L) isolated from traditional fermented yak’s milk in Tibet, China, and Lactobacillus paracasei supsp. paracasei M5L (L. paracasei M5L)isolated from kumiss in Sinkiang, China was used as control. The effects of live bacteria, cell wall and genomic DNA\\u000a of

Yanfeng Tuo; Lanwei Zhang; Xue Han; Ming Du; Yingchun Zhang; Huaxi Yi; Weiqin Zhang; Yuehua Jiao

2011-01-01

4

Comparative genomics of Lactobacillus  

Microsoft Academic Search

The genus Lactobacillus includes a diverse group of bacteria consisting of many species that are associated with fermentations of plants, meat or milk. In addition, various lactobacilli are natural inhabitants of the intestinal tract of humans and other animals. Finally, several Lactobacillus strains are marketed as probiotics as their consumption can confer a health benefit to host. Presently, 154 Lactobacillus

R. Kant; R. J. Siezen; Vos de W. M

2011-01-01

5

Lactobacillus mudanjiangensis sp. nov., Lactobacillus songhuajiangensis sp. nov. and Lactobacillus nenjiangensis sp. nov., isolated from Chinese traditional pickle and sourdough.  

PubMed

Three Gram-stain-positive bacterial strains, 11050(T), 7-19(T) and 11102(T), were isolated from traditional pickle and sourdough in Heilongjiang Province, China. These bacteria were characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, dnaK gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, DNA-DNA hybridization and an analysis of phenotypic features. Strain 11050(T) belonged to the Lactobacillus plantarum species group and shared 98.0-98.4?% 16S rRNA gene sequence similarities and 84.7-88.9?% dnaK gene sequence similarities with type strains of Lactobacillus plantarum subsp. plantarum, Lactobacillus plantarum subsp. argentoratensis, Lactobacillus pentosus, Lactobacillus paraplantarum, Lactobacillus fabifermentans and Lactobacillus xiangfangensis and had 75.9-80.7?% pheS gene sequence similarities and 90.7-92.5?% rpoA gene sequence similarities with Lactobacillus plantarum subsp. plantarum LMG 6907(T), Lactobacillus plantarum subsp. argentoratensis LMG 9205, Lactobacillus pentosus LMG 10755(T), Lactobacillus paraplantarum LMG 16673(T), Lactobacillus fabifermentans LMG 24284(T) and Lactobacillus xiangfangensis 3.1.1(T), respectively. Strain 7-19(T) was phylogenetically related to Lactobacillus thailandensis, Lactobacillus pantheris and Lactobacillus sharpeae, having 94.1-96.7?% 16S rRNA gene sequence similarities, 71.5-82.3?% pheS gene sequence similarities and 71.2-83.4?% rpoA gene sequence similarities with type strains of Lactobacillus thailandensis, Lactobacillus pantheris and Lactobacillus sharpeae, respectively. Strain 11102(T) was phylogenetically related to Lactobacillus oligofermentans, Lactobacillus suebicus, Lactobacillus vaccinostercus and Lactobacillus hokkaidonensis. Strain 11102(T) had 99.2?% 16S rRNA gene sequence similarity, 81.3?% pheS gene sequence similarity and 96.1?% rpoA gene sequence similarity with Lactobacillus oligofermentans LMG 22743(T), respectively. Strain 11102(T) shared 96.0-96.8?% 16S rRNA gene sequence similarities, 73.3-81.0?% pheS gene sequence similarities and 74.6-76.9?% rpoA gene sequence similarities with type strains of Lactobacillus suebicus, Lactobacillus vaccinostercus and Lactobacillus hokkaidonensis, respectively. Based upon the data from polyphasic characterization obtained in the present study, three novel species, Lactobacillus mudanjiangensis sp. nov., Lactobacillus songhuajiangensis sp. nov. and Lactobacillus nenjiangensis sp. nov., are proposed and the type strains are 11050(T) (?=?LMG 27194(T)?=?CCUG 62991(T)), 7-19(T) (?=?LMG 27191(T)?=?NCIMB 14832(T)?=?CCUG 62990(T)) and 11102(T) (?=?LMG 27192(T)?=?NCIMB 14833(T)), respectively. PMID:23950151

Gu, Chun Tao; Li, Chun Yan; Yang, Li Jie; Huo, Gui Cheng

2013-12-01

6

Mucosal Lactobacillus vectored vaccines.  

PubMed

Traditional non-gastrointestinal vaccines can prevent effectively the invasion of pathogens; however, these vaccines are less effective against mucosal infections because there is not a sufficient immune response at the mucosa. Most pathogens invade via a mucosal pathway (oral, intranasal, or vaginal). It is widely accepted that Lactobacillus species play a critical role as commensals in the gastrointestinal (GI) tract. Their ability to survive in the digestive tract, their close association with the intestinal epithelium, their immunomodulatory properties and their safety even when consumed in large amounts make lactobacilli attractive candidates for live vehicles for the delivery of immunogens to the intestinal mucosa. The oral or intranasal administration of Lactobacillus-based vaccines is a promising method to control mucosal infection because these vaccines could induce strong humoral and cellular immune responses both in the blood and at mucosal sites. PMID:23322214

Yu, Qinghua; Zhu, Liqi; Kang, Haihong; Yang, Qian

2013-04-01

7

Probiotic table olives: microbial populations adhering on olive surface in fermentation sets inoculated with the probiotic strain Lactobacillus paracasei IMPC2.1 in an industrial plant.  

PubMed

This study reports the dynamics of microbial populations adhering on the surface of debittered green olives cv. Bella di Cerignola in fermentation sets inoculated with the probiotic strain Lactobacillus paracasei IMPC2.1 in different brining conditions (4% and 8% (w/v) NaCl) at room temperature and 4 degrees C. The probiotic strain successfully colonized the olive surface dominating the natural LAB population and decreasing the pH of brines to Lactobacillus coryniformis, L. paracasei, L. plantarum, L. pentosus, L. rhamnosus, L. brevis, L. mali, L. vaccinostercus, L.casei, Leuconostoc mesenteroides, Leuc. pseudomesenteroides, Lactococcus lactis, Weissella paramesenteroides, W. cibaria, Enterococcus casseliflavus group and E. italicus were identified during the whole process. In particular, L. pentosus was the most frequently isolated species and it showed a high strain diversity throughout fermentation in all processes except for the one held at 4 degrees C. Also a notable incidence of Leuc. mesenteroides on olives was highlighted in this study during all fermentation. Results indicated that the human strain L. paracasei IMPC2.1 can be considered an example of a strain used in the dual role of starter and probiotic culture which allowed the control of fermentation processes and the realization of a final probiotic product with functional appeal. PMID:20226556

De Bellis, Palmira; Valerio, Francesca; Sisto, Angelo; Lonigro, Stella Lisa; Lavermicocca, Paola

2010-05-30

8

DNA probe for lactobacillus delbrueckii  

SciTech Connect

From a genomic DNA library of Lactobacillus delbrueckii subsp. bulgaricus, a clone was isolated which complements a leucine auxotrophy of an Escherichia coli strain (GE891). Subsequent analysis of the clone indicated that it could serve as a specific DNA probe. Dot-blot hybridizations with over 40 different Lactobacillus strains showed that this clone specifically recognized L. delbrueckii subsp. delbrueckii, bulgaricus, and lactis. The sensitivity of the method was tested by using an {alpha}-{sup 32}P-labeled probe.

Delley, M.; Mollet, B.; Hottinger, H. (Nestle Research Centre, Lausanne (Switzerland))

1990-06-01

9

Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee.  

PubMed

This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu". PMID:24516438

Tajabadi, Naser; Mardan, Makhdzir; Saari, Nazamid; Mustafa, Shuhaimi; Bahreini, Rasoul; Manap, Mohd Yazid Abdul

2013-01-01

10

Comparative Genomics and Transcriptional Analysis of Prophages Identified in the Genomes of Lactobacillus gasseri, Lactobacillus salivarius, and Lactobacillus casei  

Microsoft Academic Search

Lactobacillus gasseri ATCC 33323, Lactobacillus salivarius subsp. salivarius UCC 118, and Lactobacillus casei ATCC 334 contain one (LgaI), four (Sal1, Sal2, Sal3, Sal4), and one (Lca1) distinguishable prophage se- quences, respectively. Sequence analysis revealed that LgaI, Lca1, Sal1, and Sal2 prophages belong to the group of Sfi11-like pac site and cos site Siphoviridae, respectively. Phylogenetic investigation of these newly described

Marco Ventura; Carlos Canchaya; Valentina Bernini; Eric Altermann; Rodolphe Barrangou; Stephen McGrath; Marcus J. Claesson; Yin Li; Sinead Leahy; Carey D. Walker; Ralf Zink; Erasmo Neviani; Jim Steele; Jeff Broadbent; Todd R. Klaenhammer; Gerald F. Fitzgerald; Paul W. O'Toole; Douwe van Sinderen

2006-01-01

11

Genome Sequence of Lactobacillus versmoldensis KCTC 3814  

PubMed Central

Lactobacillus versmoldensis KCTC 3814 was isolated from raw fermented poultry salami. The species was present in high numbers and frequently dominated the lactic acid bacteria (LAB) populations of the products. Here, we announce the draft genome sequence of Lactobacillus versmoldensis KCTC 3814, isolated from poultry salami, and describe major findings from its annotation.

Kim, Dae-Soo; Choi, Sang-Haeng; Kim, Dong-Wook; Kim, Ryong Nam; Nam, Seong-Hyeuk; Kang, Aram; Kim, Aeri; Park, Hong-Seog

2011-01-01

12

Diversity of stress tolerance in Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus paraplantarum: A multivariate screening study.  

PubMed

Sixty-three strains of the taxonomically related species Lactobacillus plantarum subsp. plantarum, L. plantarum subsp. argentoratensis, L. paraplantarum and L. pentosus isolated from sourdoughs and other food and non-food sources and 14 strains of other members of the genus Lactobacillus were screened for their tolerance of acid, alkaline, heat, oxidative, osmotic, detergent and starvation stresses in order to evaluate the diversity of stress response. Most strains of the L. plantarum group were highly tolerant of acid, alkaline and osmotic stress and highly sensitive to detergent stress, while a larger diversity was found for other stress. Multivariate analysis allowed grouping the strains in clusters with similar response patterns. Stress response patterns in the L. plantarum group were similar to those of species of the L. casei/L. paracasei group but clearly different from those of other mesophilic Lactobacillus. No relationship was found between grouping obtained on the basis of stress response patterns and by genotypic fingerprinting (rep-PCR), nor with the taxonomic position or isolation source of the strains. Further experiments with selected strains showed that exponential phase cells were generally but not always more sensitive than stationary phase cells. The ability to grow under stressful conditions showed a slightly better correlation with the ecological conditions prevailing in the isolation niches of the strains. This study will be the basis for further investigations to identify and exploit the basis of diversity in the stress response of lactic acid bacteria. PMID:21035223

Parente, Eugenio; Ciocia, Felicia; Ricciardi, Annamaria; Zotta, Teresa; Felis, Giovanna E; Torriani, Sandra

2010-12-15

13

Discrimination of Dairy Industry Isolates of the Lactobacillus casei Group  

Microsoft Academic Search

Lactobacilli are a major part of the microflora of the gut and of many fermented dairy products, and are found in a variety of environments. Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus zeae form a closely related taxonomic group within the facultatively heterofermentative lac- tobacilli. The classification and nomenclature of these bacteria are controversial. In this study, relationships between

A. R. Desai; N. P. Shah; I. B. Powell

2006-01-01

14

Selective enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and propionibacteria.  

PubMed

Nineteen bacteriological media were evaluated to assess their suitability to selectively enumerate Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, bifidobacteria, and propionibacteria. Bacteriological media evaluated included Streptococcus thermophilus agar, pH modified MRS agar, MRS-vancomycine agar, MRS-bile agar, MRS-NaCl agar, MRS-lithium chloride agar, MRS-NNLP (nalidixic acid, neomycin sulfate, lithium chloride and paramomycine sulfate) agar, reinforced clostridial agar, sugar-based (such as maltose, galactose, sorbitol, manitol, esculin) media, sodium lactate agar, arabinose agar, raffinose agar, xylose agar, and L. casei agar. Incubations were carried out under aerobic and anaerobic conditions at 27, 30, 37, 43, and 45 degrees C for 24, 72 h, and 7 to 9 d. S. thermophilus agar and aerobic incubation at 37 degrees C for 24 h were suitable for S. thermophilus. L. delbrueckii ssp. bulgaricus could be enumerated using MRS agar (pH 4.58 or pH 5.20) and under anaerobic incubation at 45 degrees C for 72 h. MRS-vancomycine agar and anaerobic incubation at 43 degrees C for 72 h were suitable to enumerate L. rhamnosus. MRS-vancomycine agar and anaerobic incubation at 37 degrees C for 72 h were selective for L. casei. To estimate the counts of L. casei by subtraction method, counts of L. rhamnosus on MRS-vancomycine agar at 43 degrees C for 72 h under anaerobic incubation could be subtracted from total counts of L. casei and L. rhamnosus enumerated on MRS-vancomycine agar at 37 degrees C for 72 h under anaerobic incubation. L. acidophilus could be enumerated using MRS-agar at 43 degrees C for 72 h or Basal agar-maltose agar at 43 degrees C for 72 h or BA-sorbitol agar at 37 degrees C for 72 h, under anaerobic incubation. Bifidobacteria could be enumerated on MRS-NNLP agar under anaerobic incubation at 37 degrees C for 72 h. Propionibacteria could be enumerated on sodium lactate agar under anaerobic incubation at 30 degrees C for 7 to 9 d. A subtraction method was most suitable for counting propionibacteria in the presence of other lactic acid bacteria from a product. For this method, counts of lactic bacteria at d 3 on sodium lactate agar under anaerobic incubation at 30 degrees C were subtracted from counts at d 7 of lactic bacteria and propionibacteria. PMID:12906045

Tharmaraj, N; Shah, N P

2003-07-01

15

Systematics of the Lactobacillus population on rat intestinal mucosa with special reference to Lactobacillus reuteri  

Microsoft Academic Search

The systematics of theLactobacillus population of the intestines of 88 different rats was studied; 80 rats had been fed on fermented oat-meal soup (Molin et al. 1992). One-hundred-twenty-twoLactobacillus strains from the intestinal mucosa were phenotypically classified together with twenty-eight reference strains ofLactobacillus andLeuconostoc, using 49 unit characters. Data were examined using Jaccard coefficient, and unweighted pair group algorithm with arithmetic

G. Molin; M. L. Johansson; M. Ståhl; S. Ahrné; R. Andersson; B. Jeppsson; S. Bengmark

1992-01-01

16

Lactobacillus oeni sp. nov., from wine.  

PubMed

Ten Lactobacillus strains, previously isolated from different Bobal grape wines from the Utiel-Requena Origin Denomination of Spain, were characterized phylogenetically, genotypically and phenotypically. The 16S rRNA genes were sequenced and phylogenetic analysis showed that they form a tight phylogenetic clade that is closely related to reference strains Lactobacillus satsumensis NRIC 0604T, 'Lactobacillus uvarum' 8 and Lactobacillus mali DSM 20444T. DNA-DNA hybridization results confirmed the separation of the strains from other Lactobacillus species. Genotypically, the strains could be differentiated from their closest neighbours by 16S amplified rDNA restriction analysis and random amplified polymorphic DNA patterns. The strains were Gram-staining-positive, facultatively anaerobic rods that did not exhibit catalase activity. Phenotypically, they could be distinguished from their closest relatives by several traits such as their inabilities to grow at pH 3.3, to ferment sucrose, amygdalin and arbutin or to hydrolyse aesculin. The characteristics of the ten wine isolates suggest that they represent a novel species, for which the name Lactobacillus oeni sp. nov. is proposed. The type strain is 59bT (=CECT 7334T=DSM 19972T). PMID:19567555

Mañes-Lázaro, Rosario; Ferrer, Sergi; Rosselló-Mora, Ramón; Pardo, Isabel

2009-08-01

17

Production of lactic acid from soybean stalk hydrolysate with Lactobacillus sake and Lactobacillus casei  

Microsoft Academic Search

In order to make full use of soybean stalk produced in large quantity annually in China, a process is proposed for production of lactic acid from soybean stalk hydrolysate with Lactobacillus sake and Lactobacillus casei. Experiments were conducted using the proposed process and experimental results indicate that the potential of 242mg(gstalk)?1 fermentable sugar is released from hydrolysate through enzymatic saccharication

Zhong Xu; Qunhui Wang; Peng Wang; Guishi Cheng; Yongzhen Ji; Zhaohua Jiang

2007-01-01

18

Isolation and Characterization of Lactobacillus bulgaricus and Lactobacillus casei from Various Foods  

Microsoft Academic Search

The aim of this study was to determine Lactobacillus bulgaricus and Lactobacillus casei isolated from yoghurt, different kinds of cheese and a traditional food named 'tarhana' (a fermented food made of a mixture of cereal, yoghurt and thyme), and to determine the antimicrobial activity and antibiotic resistance of these isolates. The identity of the culture was based on characteristics of

Özlem ERDO

19

Hydrolysis of muscle myofibrillar proteins by Lactobacillus curvatus and Lactobacillus sake  

Microsoft Academic Search

Proteolytic enzyme activities of whole cells and cell free extracts (CFE) of Lactobacillus curvatus CECT 904 and Lactobacillus sake CECT 4808 were characterised using synthetic chromogenic compounds and myofibrillar proteins as substrates. The hydrolytic action was monitored by SDS–PAGE and reverse phase-HPLC analyses. The CFE of L. sake partially contributed, together with muscle enzymes, to the initial hydrolysis of myofibrillar

Yolanda Sanz; Silvina Fadda; Graciela Vignolo; M-Concepción Aristoy; Guillermo Oliver; Fidel Toldrá

1999-01-01

20

Genotypic diversity of stress response in Lactobacillus plantarum, Lactobacillus paraplantarum and Lactobacillus pentosus.  

PubMed

Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus paraplantarum are three closely related species which are widespread in food and non-food environments, and are important as starter bacteria or probiotics. In order to evaluate the phenotypic diversity of stress tolerance in the L. plantarum group and the ability to mount an adaptive heat shock response, the survival of exponential and stationary phase and of heat adapted exponential phase cells of six L. plantarum subsp. plantarum, one L. plantarum subsp. argentoratensis, one L. pentosus and two L. paraplantarum strains selected in a previous work upon exposure to oxidative, heat, detergent, starvation and acid stresses was compared to that of the L. plantarum WCFS1 strain. Furthermore, to evaluate the genotypic diversity in stress response genes, ten genes (encoding for chaperones DnaK, GroES and GroEL, regulators CtsR, HrcA and CcpA, ATPases/proteases ClpL, ClpP, ClpX and protease FtsH) were amplified using primers derived from the WCFS1 genome sequence and submitted to restriction with one or two endonucleases. The results were compared by univariate and multivariate statistical methods. In addition, the amplicons for hrcA and ctsR were sequenced and compared by multiple sequence alignment and polymorphism analysis. Although there was evidence of a generalized stress response in the stationary phase, with increase of oxidative, heat, and, to a lesser extent, starvation stress tolerance, and for adaptive heat stress response, with increased tolerance to heat, acid and detergent, different growth phases and adaptation patterns were found. Principal component analysis showed that while heat, acid and detergent stresses respond similarly to growth phase and adaptation, tolerance to oxidative and starvation stresses implies completely unrelated mechanisms. A dendrogram obtained using the data from multilocus restriction typing (MLRT) of stress response genes clearly separated two groups of L. plantarum strains from the other species but there was no correlation between genotypic grouping and grouping obtained on the basis of the stress response pattern, nor with the phylograms obtained from hrcA and ctsR sequences. Differences in sequence in L. plantarum strains were mostly due to single nucleotide polymorphisms with a high frequency of synonymous nucleotide changes and, while hrcA was characterized by an excess of low frequency polymorphism, very low diversity was found in ctsR sequences. Sequence alignment of hrcA allowed a correct discrimination of the strains at the species level, thus confirming the relevance of stress response genes for taxonomy. PMID:22704047

Ricciardi, Annamaria; Parente, Eugenio; Guidone, Angela; Ianniello, Rocco Gerardo; Zotta, Teresa; Abu Sayem, S M; Varcamonti, Mario

2012-07-01

21

Lactobacillus  

MedlinePLUS

Natural Medicines Comprehensive Database rates effectiveness based on scientific evidence according to the following scale: Effective, Likely ... also been used. For preventing and shortening the duration of diarrhea in newborns within the first year ...

22

Complete Genome Sequence of Lactobacillus helveticus H10 ?  

PubMed Central

Lactobacillus helveticus strain H10 was isolated from traditional fermented milk in Tibet, China. We sequenced the whole genome of strain H10 and compared it to the published genome sequence of Lactobacillus helveticus DPC4571.

Zhao, Wenjing; Chen, Yongfu; Sun, Zhihong; Wang, Jicheng; Zhou, Zhemin; Sun, Tiansong; Wang, Lei; Chen, Wei; Zhang, Heping

2011-01-01

23

Survival of Lactobacillus species (strain GG) in human gastrointestinal tract  

Microsoft Academic Search

A newly isolated strain of a species ofLactobacillus of human origin, designated GG (Lactobacillus GG), has been studied to determine its ability to survive in the human gastrointestinal tract. When fed to 76 volunteers as a frozen concentrate or as a fermented preparation in milk or whey,Lactobacillus GG was recovered in the feces of all subjects receiving the fermented milk

Barry R. Goldin; Sherwood L. Gorbach; Maija Saxelin; Susan Barakat; Lisa Gualtieri; Seppo Salminen

1992-01-01

24

Draft Genome Sequence of Lactobacillus pobuzihii E100301T  

PubMed Central

Lactobacillus pobuzihii E100301T is a novel Lactobacillus species previously isolated from pobuzihi (fermented cummingcordia) in Taiwan. Phylogenetically, this strain is closest to Lactobacillus acidipiscis, but its phenotypic characteristics can be clearly distinguished from those of L. acidipiscis. We present the draft genome sequence of strain L. pobuzihii E100301T.

Chiu, Chi-ming; Chang, Chi-huan; Pan, Shwu-fen; Wu, Hui-chung; Li, Shiao-wen; Chang, Chuan-hsiung; Lee, Yun-shien; Chiang, Chih-ming

2013-01-01

25

Lactobacillus saerimneri and Lactobacillus ruminis: novel human-derived probiotic strains with immunomodulatory activities  

PubMed Central

Human-derived lactobacilli were isolated from fecal samples of healthy volunteers. Forty-six isolates from different volunteers were selected and investigated for their immunomodulatory properties. Conditioned medium from each isolate was assessed for its effect on tumor necrosis factor (TNF) production in lipopolysaccharide (LPS) - activated THP-1 monocytes. Of 46 Lactobacillus isolates, 12 significantly inhibited TNF production in varying magnitude. Lactobacillus strain TH58 displayed the most potent TNF - inhibitory activity (70% inhibition). In contrast, Lactobacillus strain TH14 exhibited immunostimulatory property by activating TNF production in THP-1 monocytes. Lactobacillus TH14 induced NF-?B activation in the absence of LPS stimulation, whereas Lactobacillus TH58 had no effect on NF-?B signaling, irrespective of LPS stimulation. Strain TH58 was identified as Lactobacillus saerimneri and strain TH14 as Lactobacillus ruminis by sequence analysis of the16S rRNA gene. This is the first report of a human isolate of L. saerimneri with TNF inhibitory activity and L. ruminis, an indigenous species to humans, with TNF stimulatory activity. Our data suggest the potential use of these two strains as immunoprobiotic candidates.

Taweechotipatr, Malai; Iyer, Chandra; Spinler, Jennifer K.; Versalovic, James; Tumwasorn, Somying

2014-01-01

26

Cell-Free Supernatants from Probiotic Lactobacillus casei and Lactobacillus rhamnosus GG Decrease Colon Cancer Cell Invasion In Vitro  

Microsoft Academic Search

Probiotics have been shown to have a preventative role in colorectal carcinogenesis but research concerning their prophylactic potential in the later stages of colorectal cancer, specifically metastasis is limited. This study explored the potential of cell-free supernatants (CFS) from 2 probiotic Lactobacillus sp., Lactobacillus casei and Lactobacillus rhamnosus GG, to inhibit colon cancer cell invasion by influencing matrix metalloproteinase-9 (MMP-9)

Juanita Escamilla; Vatsala Maitin

2012-01-01

27

Lactobacillus helveticus: the proteolytic system  

PubMed Central

Lactobacillus helveticus is one of the species of lactic acid bacteria (LAB) most commonly used in the production of fermented milk beverages and some types of hard cheese. The versatile nature of this bacterium is based on its highly efficient proteolytic system consisting of cell-envelope proteinases (CEPs), transport system and intracellular peptidases. Besides use of L. helveticus in cheese processing, the production of fermented milk preparations with health promoting properties has become an important industrial application. Studies have shown that fermented dairy products are able to decrease blood pressure, stimulate the immune system, promote calcium absorption, and exert an anti-virulent effect against pathogens. These beneficial effects are produced by a variety of peptides released during the hydrolysis of milk proteins by the proteolytic system of L. helveticus, which provides the bacterium with its nutritional requirements for growth. In recent years, studies have focused on understanding the factors that affect the kinetics of milk protein hydrolysis by specific strains and have concentrated on the effect of pH, temperature, growth phase, and matrix composition on the bacterial enzymatic system. This review focuses on the role of the proteolytic system of L. helveticus in the production of bioactive compounds formed during fermentation of dairy products. Taking advantage of the powerful proteolytic system of this bacterium opens up future opportunities to search for novel food-derived compounds with potential health promoting properties.

Griffiths, M. W.; Tellez, A. M.

2012-01-01

28

Hydrolysis of Pork Muscle Sarcoplasmic Proteins by Lactobacillus curvatus and Lactobacillus sake  

Microsoft Academic Search

Lactobacillus curvatus CECT 904 and Lactobacillus sake CECT 4808 were selected on the basis of their proteolytic activities against synthetic substrates. Further, the effects of whole cells, cell extracts, and a combination of both enzymatic sources on muscle sarcoplasmic proteins were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography anal- yses. Strains of both species displayed

Silvina Fadda; Yolanda Sanz; Graciela Vignolo; M. concepcio N Aristoy; Guillermo Oliver; Fidel Toldra

1999-01-01

29

Ensiling whole-crop wheat and corn in large containers with Lactobacillus plantarum and Lactobacillus buchneri  

Microsoft Academic Search

  The effect of applying Lactobacillus buchneri, alone or in combination with Lactobacillus plantarum, at ensiling, on the aerobic stability of wheat and corn silages was studied in 50-l plastic containers. Treatments comprised\\u000a control (no additives), L. plantarum, L. buchneri and a combination of L. plantarum+L. buchneri. After 3 months of storage, the wheat silages treated with L. buchneri had higher

ZG Weinberg; G Ashbell; Y Hen; A Azrieli; G Szakacs; I Filya

2002-01-01

30

Lactobacillus kefirgranum sp. nov. and Lactobacillus parakefir sp. nov., Two New Species from Kefir Grains  

Microsoft Academic Search

Twelve strains of homofermentative lactobacilli and two strains of heterofermentative lactobacilli were isolated from kefir grains by using R-CW agar medium. The physiological and biochemical characteristics, DNA guanine-plus-cytosine contents, and levels of DNA-DNA relatedness of these isolates and previously described lactobacilli were compared. Our results indicated that two new species, Lactobacillus kefirgranurn and Lactobacillus parakefir, could be distinguished. The type

S. TAKIZAWA; S. KOJIMA; S. TAMURA; S. FUJINAGA; Y. BENNO

1994-01-01

31

Manganese acquisition by Lactobacillus plantarum  

SciTech Connect

Lactobacillus plantarum has an unusually high Mn(II) requirement for growth and accumulated over 30 mM intracellular Mn(II). The acquisition of Mn(II) by L. plantarum occurred via a specific active transport system powered by the transmembrane proton gradient. The Mn(II) uptake system has a K/sub m/ of 0.2 ..mu..M and a V/sub max/ of 24 nmol mg/sup -1/ of protein min/sup -1/. Above a medium Mn(II) concentration of 200 ..mu..M, the intracellular Mn(II) level was independent of the medium Mn(II) and unresponsive to oxygen stresses but was reduced by phosphate limitation. At a pH of 5.5, citrate, isocitrate, and cis-aconitate effectively promoted MN(II) uptake, although measurable levels of 1,5-(/sup 14/C)citrate were not accumulated. When cells were presented with equimolar Mn(II) and Cd(II), Cd(II) was preferentially taken up by the Mn(II) transport system. Both Mn(II) and Cd(II) uptake were greatly increased by Mn(II) starvation. Mn(II) uptake by Mn(II)-starved cells was subject to a negative feedback regulatory mechanism functioning less than 1 min after exposure of the cells to Mn(II) and independent of protein synthesis. When presented with a relatively large amount of exogenous Mn(II), Mn(II)-starved cells exhibited a measurable efflux of their internal Mn(II), but the rate was only a small fraction of the maximal Mn(II) uptake rate.

Archibald, F.S.; Duong, M.N.

1984-04-01

32

CD4 detected from Lactobacillus helps understand the interaction between Lactobacillus and HIV.  

PubMed

Human immunodeficiency virus (HIV) preferentially infects and destroys CD4+ cells and leads to a gradual decline in the number of CD4 cells. Despite evidence that probiotics increase CD4+ T lymphocytes in patients with HIV/acquired immunodeficiency syndrome (AIDS) and lower the risk of HIV transmission, little is known about the detailed mechanism underlying these effects. In this study, we investigated the cell surface protein of Lactobacillus and its role in blocking HIV-1 transmission by lactobacilli. Using reverse transcription-polymerase chain reaction (RT-PCR), immunofluorescence, and flow cytometry (fluorescence-activated cell sorting, FACS), we detected the CD4 receptor on the surface of Lactobacillus. Monoclonal antibody (mAb) for the CD4 receptor could partially inhibit HIV-1 binding to Lactobacillus. In addition, Lactobacillus could decrease HIV-1 pseudovirus infection of TZM-bl cells in vitro by 60-70%. Our data suggest that Lactobacillus can use this receptor to bind HIV and block HIV infection. This may in turn increase the CD4 T lymphocyte count in patients with HIV. These data provide direct evidence that Lactobacillus expresses the CD4 receptor and utilizes it to block HIV transmission. PMID:23318049

Su, Yan; Zhang, Baojiang; Su, Lingling

2013-06-12

33

Differentiation of Lactobacillus Species by Molecular Typing  

Microsoft Academic Search

A total of 64 type, reference, clinical, health food, and stock isolates of microaerophilic Lactobacillus species were examined by restriction fragment length polymorphisms. Of particular interest were members of six of the eight species most commonly recovered from the vaginas of healthy premenopausal women, namely, Lactobacil- lus jensenii, L. casei, L. rhamnosus, L. acidophilus, L. plantarum, and L. fermentum. Six

WEI ZHONG; KEVIN MILLSAP; HANNA BIALKOWSKA-HOBRZANSKA; GREGOR REID

1998-01-01

34

Lactobacillus assisted synthesis of titanium nanoparticles  

NASA Astrophysics Data System (ADS)

An eco-friendly lactobacillus sp. (microbe) assisted synthesis of titanium nanoparticles is reported. The synthesis is performed at room temperature. X-ray and transmission electron microscopy analyses are performed to ascertain the formation of Ti nanoparticles. Individual nanoparticles as well as a number of aggregates almost spherical in shape having a size of 40 60 nm are found.

Prasad, K.; Jha, Anal K.; Kulkarni, A. R.

2007-05-01

35

The peptide hydrolase system of Lactobacillus reuteri  

Microsoft Academic Search

Peptide hydrolase system of Lactobacillus reuteri CRL 1098, a lactic acid bacteria of sourdough origin, was investigated. This microorganism has a broad range of peptidases consisting of an active aminopeptidase, X-Prolyl-dipeptidylaminopeptidase, dipeptidase and tripeptidase. Aminopeptidase, iminopeptidase and endopeptidase are most likely located in the cytoplasmic fraction showing no detectable association with the cell membrane, while dipeptidase and tripeptidase are mainly

G Rollán

2001-01-01

36

Amino acid transport in Lactobacillus helveticus  

Microsoft Academic Search

Amino acid transport in Lactobacillus helveticus was analyzed. Strain specificity of amino acid transport was speculated between L. helveticus NCDO2712 and SBT2171. Glucose energized L. helveticus NCDO2712 actively transported and accumulated the essential and growth stimulating amino acids (leucine, isoleucine, valine, threonine, lysine, aspartic acid, glutamic acid, tyrosine, arginine, and histidine). Uptake of proline, phenylalanine and tryptophan was not observed.

Hadjime Nakajima; Edmund R. S. Kunji; Bert Poolman; Wil N. Konings

1998-01-01

37

Draft Genome Sequence of Lactobacillus rhamnosus 2166  

PubMed Central

In this report, we present a draft sequence of the genome of Lactobacillus rhamnosus strain 2166, a potential novel probiotic. Genome annotation and read mapping onto a reference genome of L. rhamnosus strain GG allowed for the identification of the differences and similarities in the genomic contents and gene arrangements of these strains.

Melnikov, Vyacheslav G.; Kosarev, Igor V.; Abramov, Vyacheslav M.

2014-01-01

38

Draft Genome Sequence of Lactobacillus crispatus 2029.  

PubMed

This report describes a draft genome sequence of Lactobacillus crispatus 2029. The reads generated by the Ion Torrent PGM were assembled into contigs with a total size of 2.2 Mb. The data were annotated using the NCBI GenBank and RAST servers. A comparison with the reference strain revealed specific features of the genome. PMID:24558253

Karlyshev, Andrey V; Melnikov, Vyacheslav G; Khlebnikov, Valentin C; Abramov, Vyacheslav M

2014-01-01

39

21 CFR 184.1924 - Urease enzyme preparation from Lactobacillus fermentum.  

Code of Federal Regulations, 2010 CFR

...false Urease enzyme preparation from Lactobacillus fermentum. 184.1924 Section...1924 Urease enzyme preparation from Lactobacillus fermentum. (a) This enzyme preparation...nonpathogenic, nontoxicogenic bacterium Lactobacillus fermentum. It contains...

2009-04-01

40

21 CFR 184.1924 - Urease enzyme preparation from Lactobacillus fermentum.  

Code of Federal Regulations, 2010 CFR

...true Urease enzyme preparation from Lactobacillus fermentum. 184.1924 Section...1924 Urease enzyme preparation from Lactobacillus fermentum. (a) This enzyme preparation...nonpathogenic, nontoxicogenic bacterium Lactobacillus fermentum. It contains...

2010-01-01

41

Effect of probiotic Lactobacillus strains in children with atopic dermatitis  

Microsoft Academic Search

Background: Recent studies suggest that oral bacteriotherapy with probiotics might be useful in the management of atopic dermatitis (AD). Objective: The purpose of this investigation was to evaluate the clinical and anti-inflammatory effect of probiotic supplementation in children with AD. Methods: In a double-blind, placebo-controlled, crossover study, 2 probiotic Lactobacillus strains (lyophilized Lactobacillus rhamnosus 19070-2 and Lactobacillus reuteri DSM 122460)

Vibeke Rosenfeldt; Eva Benfeldt; Susanne Dam Nielsen; Kim Fleischer Michaelsen; Dorthe Lisbeth Jeppesen; Niels Henrik Valerius; Anders Paerregaard

2003-01-01

42

Physicochemical and biochemical characterization of biosurfactants released by Lactobacillus strains  

Microsoft Academic Search

Biosurfactants from Lactobacillus casei subsp. rhamnosus 36 and ATCC 7469, Lactobacillus fermentum B54 and Lactobacillus acidophilus RC14 were isolated from bacteria in their mid-exponential (4–5 h) and stationary growth phases (18 h) and physicochemical and biochemical properties of the freeze-dried biosurfactants from both growth phases were compared. The mid-exponential and stationary phase biosurfactants were similar in their surface activities, but

Martine M. C. Velraeds; Henny C. van der Mei; Gregor Reid; Henk J. Busscher

1996-01-01

43

Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products.  

PubMed

A total of 234 LAB isolates from Brazilian food products were initially screened for their ability to survive at pH 2.0. Fifty one of the isolates survived and were selected. They were characterized by phenotypic methods, rep-PCR and identified using 16S rRNA gene sequencing as Lactobacillus fermentum (34 isolates), Lactobacillus plantarum (10) and Lactobacillus brevis (7). Based on being either highly tolerant to bile, showing an ability for auto-aggregation and/or hydrophobic properties, one L. fermentum (CH58), three L. plantarum (CH3, CH41 and SAU96) and two L. brevis (SAU105 and FFC199) were selected. The highest co-aggregation ability with Escherichia coli was observed to L. plantarum CH41. L. brevis SAU105 and FFC199 and L. fermentum CH58 exhibited antagonistic activity towards the pathogens Listeria monocytogenes and Staphylococcus aureus. L. plantarum CH3 and CH41 and L. brevis FFC199 showed adhesion ability to Caco-2 cells (1.6, 1.1 and 0.9%, respectively) similar to the commercial probiotic, Lactobacillus rhamnosus GG (1.5%). They were able to increase the transepithelial electrical resistance (TEER) of Caco-2 cells over 24 h (p < 0.05). The present work showed that the probiotic characteristics were strain-specific and that the isolates L. plantarum CH3 and CH41 (cocoa) and L. brevis FFC199 (cauim) exhibited potential probiotics properties. PMID:23764216

Ramos, Cíntia Lacerda; Thorsen, Line; Schwan, Rosane Freitas; Jespersen, Lene

2013-10-01

44

Comparative Genomics and Transcriptional Analysis of Prophages Identified in the Genomes of Lactobacillus gasseri, Lactobacillus salivarius, and Lactobacillus casei†  

PubMed Central

Lactobacillus gasseri ATCC 33323, Lactobacillus salivarius subsp. salivarius UCC 118, and Lactobacillus casei ATCC 334 contain one (LgaI), four (Sal1, Sal2, Sal3, Sal4), and one (Lca1) distinguishable prophage sequences, respectively. Sequence analysis revealed that LgaI, Lca1, Sal1, and Sal2 prophages belong to the group of Sfi11-like pac site and cos site Siphoviridae, respectively. Phylogenetic investigation of these newly described prophage sequences revealed that they have not followed an evolutionary development similar to that of their bacterial hosts and that they show a high degree of diversity, even within a species. The attachment sites were determined for all these prophage elements; LgaI as well as Sal1 integrates in tRNA genes, while prophage Sal2 integrates in a predicted arginino-succinate lyase-encoding gene. In contrast, Lca1 and the Sal3 and Sal4 prophage remnants are integrated in noncoding regions in the L. casei ATCC 334 and L. salivarius UCC 118 genomes. Northern analysis showed that large parts of the prophage genomes are transcriptionally silent and that transcription is limited to genome segments located near the attachment site. Finally, pulsed-field gel electrophoresis followed by Southern blot hybridization with specific prophage probes indicates that these prophage sequences are narrowly distributed within lactobacilli.

Ventura, Marco; Canchaya, Carlos; Bernini, Valentina; Altermann, Eric; Barrangou, Rodolphe; McGrath, Stephen; Claesson, Marcus J.; Li, Yin; Leahy, Sinead; Walker, Carey D.; Zink, Ralf; Neviani, Erasmo; Steele, Jim; Broadbent, Jeff; Klaenhammer, Todd R.; Fitzgerald, Gerald F.; O'Toole, Paul W.; van Sinderen, Douwe

2006-01-01

45

A selective differential medium for Lactobacillus plantarum.  

PubMed

The quantification of exogenous lactobacilli in faecal samples is frequently required for the evaluation of the intestinal colonization by probiotic bacteria. In this study, a selective and differential medium, designated LPSM, was developed for the culture of exogenous Lactobacillus plantarum. In quantitative assays, LPSM showed a sensitivity similar to those of enriched and Lactobacillus-adapted media. The presence of ciprofloxacin made LPSM inhibitory to most intestinal bacteria, including endogenous acid lactic bacteria, whereas exogenous L. plantarum strains grew producing a yellow color caused by acid production from sorbitol in the presence of bromocresol purple. The results showed that LPSM is suitable for detection and enumeration of L. plantarum in faecal samples. PMID:16554099

Bujalance, Carmen; Jiménez-Valera, Maria; Moreno, Encarnacion; Ruiz-Bravo, Alfonso

2006-09-01

46

Binding specificity of Lactobacillus to glycolipids.  

PubMed

Lactobacillus, a representative useful bacterium, in the intestinal tract was found to bind to some specific glycosphingolipids, like the pathogenic intestinal bacteria. Thin layer chromatography overlay assays using rabbit antiserum against Lactobacillus casei revealed that the bacteria bound to GA1 and trihexosylceramide strongly, but not to any gangliosides. The bacteria generally bound to glycosphingolipids having short sugar chains and galactosyl moiety in the non-reducing terminal. L.casei did not bind to GM1, but bound to the product after sialidase treatment, GA1. This indicated that sialic acid inhibited the adhesion of L.casei to tissues. L.casei actually bound nonacid glycosphingolipids but not acid glycosphingolipids extracted from the small intestinal mucosa of rats. PMID:8912650

Yamamoto, K; Miwa, T; Taniguchi, H; Nagano, T; Shimamura, K; Tanaka, T; Kumagai, H

1996-11-01

47

Lactobacillus reuteri CRL1098 Produces Cobalamin  

Microsoft Academic Search

We found that Lactobacillus reuteri CRL1098, a lactic acid bacterium isolated from sourdough, is able to produce cobalamin. The sugar-glycerol cofermentation in vitamin B12-free medium showed that this strain was able to reduce glycerol through a well-known cobalamin-dependent reaction with the formation of 1,3-pro- panediol as a final product. The cell extract of L. reuteri corrected the coenzyme B12 requirement

María P. Taranto; J. L. Vera; J. Hugenholtz; G. F. De Valdez; F. Sesma

2003-01-01

48

Susceptibility of Lactobacillus spp. to antimicrobial agents.  

PubMed

Bacteria used as probiotics or in starter cultures may serve as hosts of antibiotic resistance genes, which can be transferred to pathogenic bacteria. Before launching a starter culture or a probiotic product into the market, it is therefore important to verify that the single bacterial isolates (strains) do not contain transferable resistance genes. A study has been undertaken to establish the levels of susceptibility of Lactobacillus spp. to various antimicrobial agents. This is a prerequisite for differentiating putative transferable resistance from natural resistance. A selection of 62 strains has been screened with the use of the Etest (ABBiodisk, Stockholm, Sweden) for their susceptibility to 25 antimicrobial agents. The strains belonged to the following species: Lactobacillus plantarum/pentosus, L. rhamnosus, L. paracasei, L. sakei, L. curvatus and species of the L. acidophilus group: L. johnsonii, L. crispatus, L. gasseri, and L. acidophilus. The results from the Etests have shown that the level of susceptibility to the antimicrobial agents is species-dependent. For the following antimicrobial agents, susceptibility varied several folds between species: vancomycin, teicoplanin, tetracycline, norfloxacin, ciprofloxacin, fusidic acid, and clindamycin. The differences between the species were more subtle for the rest of the tested antimicrobial agents. On the basis of the result, it was possible to suggest minimal inhibition concentrations (MICs) for the individual Lactobacillus species to be used as a microbiological breakpoint when screening strains for transferable resistance genes. PMID:12505455

Danielsen, Morten; Wind, Anette

2003-01-26

49

Hydrolysis of muscle myofibrillar proteins by Lactobacillus curvatus and Lactobacillus sake.  

PubMed

Proteolytic enzyme activities of whole cells and cell free extracts (CFE) of Lactobacillus curvatus CECT 904 and Lactobacillus sake CECT 4808 were characterised using synthetic chromogenic compounds and myofibrillar proteins as substrates. The hydrolytic action was monitored by SDS-PAGE and reverse phase-HPLC analyses. The CFE of L. sake partially contributed, together with muscle enzymes, to the initial hydrolysis of myofibrillar proteins. Whole-cells of both L. curvatus and L. sake generated peptides considered important for cured-meat taste. The peptide mapping, resulting from the action on the substrates assayed, revealed a profile of extra and intracellular enzymes. Both strains expressed strong amino acid metabolism. PMID:10634703

Sanz, Y; Fadda, S; Vignolo, G; Aristoy, M C; Oliver, G; Toldrá, F

1999-12-15

50

Decolorization of water and oil-soluble azo dyes by Lactobacillus acidophilus and Lactobacillus fermentum  

Microsoft Academic Search

The capability of Lactobacillus acidophilus and Lactobacillus fermentum to degrade azo dyes was investigated. The bacteria were incubated under anaerobic conditions in the presence of 6 µg\\/ml Methyl\\u000a Red, Ponceau BS, Orange G, Amaranth, Orange II, and Direct Blue 15; 5 µg\\/ml Sudan I and II; or 1.5 µg\\/ml Sudan III and IV\\u000a in deMann–Rogosa–Sharpe broth at 37°C for 36 h, and reduction of

Huizhong Chen; Haiyan Xu; Thomas M. Heinze; Carl E. Cerniglia

2009-01-01

51

Characterization of Reutericyclin Produced by Lactobacillus reuteri LTH2584  

Microsoft Academic Search

Lactobacillus reuteri LTH2584 exhibits antimicrobial activity that can be attributed neither to bacteriocins nor to the production of reuterin or organic acids. We have purified the active compound, named reutericyclin, to homogeneity and characterized its antimicrobial activity. Reutericyclin exhibited a broad inhibitory spec- trum including Lactobacillus spp., Bacillus subtilis, B. cereus, Enterococcus faecalis, Staphylococcus aureus, and Listeria innocua. It did

MICHAEL G. GANZLE; ALEXANDRA HOLTZEL; JENS WALTER; GUNTHER JUNG; WALTER P. HAMMES

2000-01-01

52

Biochemical and molecular characterization of a levansucrase from Lactobacillus reuteri  

Microsoft Academic Search

Lactobacillus reuteri strain 121 employs a fructosyltransferase (FTF) to synthesize a fructose polymer [a fructan of the levan type, with ?(2?6) linkages] from sucrose or raffinose. Purification of this FTF (a levansucrase), and identification of peptide amino acid sequences, allowed isolation of the first Lactobacillus levansucrase gene (lev), encoding a protein (Lev) consisting of 804 amino acids. Lev showed highest

E. Szalowska; M. J. E. C. van der Maarel; L. Dijkhuizen

2004-01-01

53

Construction of a new shuttle vector for Lactobacillus.  

PubMed

To clone the malolactic enzyme gene from Lactobacillus sp. 89, construction of a shuttle vector able to express itself in Lactobacillus sp. 89 and Escherichia coli was undertaken. The shuttle plasmid pLE16 resulted from the union of pBR328 and of the pLB10 plasmid extracted from Lactobacillus bulgaricus 10. The bacterial transformation in Lactobacillus sp. 89 was performed by electroporation, and the clones were selected on MRS medium with 30 micrograms.mL-1 chloramphenicol added. Fifty percent of the clones from Lactobacillus sp. 89 lost their resistance to chloramphenicol following 28 generations when the selection pressure was not maintained. The restriction map of pLE16 (7600 bp) was established using several restriction enzymes. PMID:1581865

Chagnaud, P; Chan Kwo Chion, C K; Duran, R; Naouri, P; Arnaud, A; Galzy, P

1992-01-01

54

Genome Sequence of the Probiotic Strain Lactobacillus rhamnosus (Formerly Lactobacillus casei) LOCK908.  

PubMed

Lactobacillus rhamnosus LOCK908, a patented probiotic strain (Polish patent no. 209987), was isolated from the feces of a healthy 6-year-old girl. Here, we present the complete genome sequence of LOCK908 and identify genes likely to be involved in the biosynthesis of exopolysaccharides (EPSs). PMID:24558250

Koryszewska-Baginska, Anna; Bardowski, Jacek; Aleksandrzak-Piekarczyk, Tamara

2014-01-01

55

Genome Sequence of the Probiotic Strain Lactobacillus rhamnosus (Formerly Lactobacillus casei) LOCK908  

PubMed Central

Lactobacillus rhamnosus LOCK908, a patented probiotic strain (Polish patent no. 209987), was isolated from the feces of a healthy 6-year-old girl. Here, we present the complete genome sequence of LOCK908 and identify genes likely to be involved in the biosynthesis of exopolysaccharides (EPSs).

Koryszewska-Baginska, Anna; Bardowski, Jacek

2014-01-01

56

Complete Genome Sequences of Lactobacillus johnsonii Strain N6.2 and Lactobacillus reuteri Strain TD1  

PubMed Central

We report here the complete genome sequences of Lactobacillus johnsonii strain N6.2, a homofermentative lactic acid intestinal bacterium, and Lactobacillus reuteri strain TD1, a heterofermentative lactic acid intestinal bacterium, both isolated from a type 1 diabetes-resistant rat model.

Leonard, Michael T.; Valladares, Ricardo B.; Ardissone, Alexandria; Gonzalez, Claudio F.; Lorca, Graciela L.

2014-01-01

57

Complete Genome Sequences of Lactobacillus johnsonii Strain N6.2 and Lactobacillus reuteri Strain TD1.  

PubMed

We report here the complete genome sequences of Lactobacillus johnsonii strain N6.2, a homofermentative lactic acid intestinal bacterium, and Lactobacillus reuteri strain TD1, a heterofermentative lactic acid intestinal bacterium, both isolated from a type 1 diabetes-resistant rat model. PMID:24812223

Leonard, Michael T; Valladares, Ricardo B; Ardissone, Alexandria; Gonzalez, Claudio F; Lorca, Graciela L; Triplett, Eric W

2014-01-01

58

Evaluation of enzymic potential for biotransformation of isoflavone phytoestrogen in soymilk by Bifidobacterium animalis, Lactobacillus acidophilus and Lactobacillus casei  

Microsoft Academic Search

Three strains of Lactobacillus acidophilus, two of Lactobacillus casei and one of Bifidobacterium were screened for ?-glucosidase activity using ?-nitrophenyl-?-d-glucopyranoside as a substrate and their potential for the breakdown of isoflavone glucosides to the biologically active aglycones in soymilk. Isoflavones quantification with HPLC and ?-glucosidase activity were performed after 0, 12, 24, 36, and 48h of incubation in soymilk at

Daniel O. Otieno; John F. Ashton; Nagendra P. Shah

2006-01-01

59

Growth Stimulation of Lactobacillus Species by Lactic Streptococci1  

PubMed Central

Cell extracts of Streptococcus species important in cheese starters stimulated the growth of Lactobacillus species common to Cheddar cheese. All Lactobacillus strains employed, with the exception of a strain of L. casei, were significantly stimulated by a strain of S. diacetilactis. L. casei was highly stimulated by both a strain of S. lactis and a strain of S. diacetilactis. The stimulant(s) was dialyzable and was partially inactivated by heat. The stimulatory principle was active at 10 C, indicating that the stimulatory effect may be influencing the growth of lactobacilli in Cheddar cheese during curing. Viable Streptococcus cells did not inhibit the growth of Lactobacillus species.

Branen, A. L.; Keenan, T. W.

1969-01-01

60

Lactobacillus reuteri CRL1098 Produces Cobalamin  

PubMed Central

We found that Lactobacillus reuteri CRL1098, a lactic acid bacterium isolated from sourdough, is able to produce cobalamin. The sugar-glycerol cofermentation in vitamin B12-free medium showed that this strain was able to reduce glycerol through a well-known cobalamin-dependent reaction with the formation of 1,3-propanediol as a final product. The cell extract of L. reuteri corrected the coenzyme B12 requirement of Lactobacillus delbrueckii subsp. lactis ATCC 7830 and allowed the growth of Salmonella enterica serovar Typhimurium (metE cbiB) and Escherichia coli (metE) in minimal medium. Preliminary genetic studies of cobalamin biosynthesis genes from L. reuteri allowed the identification of cob genes which encode the CobA, CbiJ, and CbiK enzymes involved in the cobalamin pathway. The cobamide produced by L. reuteri, isolated in its cyanide form by using reverse-phase high-pressure liquid chromatography, showed a UV-visible spectrum identical to that of standard cyanocobalamin (vitamin B12).

Taranto, Maria P.; Vera, Jose L.; Hugenholtz, Jeroen; De Valdez, Graciela F.; Sesma, Fernando

2003-01-01

61

Bile resistance mechanisms in Lactobacillus and Bifidobacterium  

PubMed Central

Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Most of the probiotic bacteria currently available in the market belong to the genera Lactobacillus and Bifidobacterium, and specific health-promoting activities, such as treatment of diarrhea or amelioration of gastrointestinal discomfort, have been attributed to them. In order to be able to survive the gastrointestinal transit and transiently colonize our gut, these bacteria must be able to counteract the deleterious action of bile salts, which are the main components of bile. Bile salts are detergent-like biological substances synthesized in the liver from cholesterol. Host enzymes conjugate the newly synthesized free bile acids in the liver with the amino acids glycine or taurine, generating conjugated bile salts. These compounds are stored in the gall bladder and they are released into the duodenum during digestion to perform their physiological function, which is the solubilization of fat coming from diet. These bile salts possess strong antimicrobial activity, since they are able to disorganize the structure of the cell membrane, as well as trigger DNA damage. This means that bacteria inhabiting our intestinal tract must have intrinsic resistance mechanisms to cope with bile salts. To do that, Lactobacillus and Bifidobacterium display a variety of proteins devoted to the efflux of bile salts or protons, to modify sugar metabolism or to prevent protein misfolding. In this manuscript, we review and discuss specific bile resistance mechanisms, as well as the processes responsible for the adaptation of bifidobacteria and lactobacilli to bile.

Ruiz, Lorena; Margolles, Abelardo; Sanchez, Borja

2013-01-01

62

Lactobacillus Decelerates Cervical Epithelial Cell Cycle Progression  

PubMed Central

We investigated cell cycle progression in epithelial cervical ME-180 cells during colonization of three different Lactobacillus species utilizing live cell microscopy, bromodeoxyuridine incorporation assays, and flow cytometry. The colonization of these ME-180 cells by L. rhamnosus and L. reuteri, originating from human gastric epithelia and saliva, respectively, was shown to reduce cell cycle progression and to cause host cells to accumulate in the G1 phase of the cell cycle. The G1 phase accumulation in L. rhamnosus-colonized cells was accompanied by the up-regulation and nuclear accumulation of p21. By contrast, the vaginal isolate L. crispatus did not affect cell cycle progression. Furthermore, both the supernatants from the lactic acid-producing L. rhamnosus colonies and lactic acid added to cell culture media were able to reduce the proliferation of ME-180 cells. In this study, we reveal the diversity of the Lactobacillus species to affect host cell cycle progression and demonstrate that L. rhamnosus and L. reuteri exert anti-proliferative effects on human cervical carcinoma cells.

Vielfort, Katarina; Weyler, Linda; Soderholm, Niklas; Engelbrecht, Mattias; Lofmark, Sonja; Aro, Helena

2013-01-01

63

Divergence in codon usage of Lactobacillus species.  

PubMed Central

We have analyzed codon usage patterns of 70 sequenced genes from different Lactobacillus species. Codon usage in lactobacilli is highly biased. Both inter-species and intra-species heterogeneity of codon usage bias was observed. Codon usage in L. acidophilus is similar to that in L. helveticus, but dissimilar to that in L. bulgaricus, L. casei, L. pentosus and L. plantarum. Codon usage in the latter three organisms is not significantly different, but is different from that in L. bulgaricus. Inter-species differences in codon usage can, at least in part, be explained by differences in mutational drift. L. bulgaricus shows GC drift, whereas all other species show AT drift. L. acidophilus and L. helveticus rarely use NNG in family-box (a set of synonymous) codons, in contrast to all other species. This result may be explained by assuming that L. acidophilus and L. helveticus, but not other species examined, use a single tRNA species for translation of family-box codons. Differences in expression level of genes are positively correlated with codon usage bias. Highly expressed genes show highly biased codon usage, whereas weakly expressed genes show much less biased codon usage. Codon usage patterns at the 5'-end of Lactobacillus genes is not significantly different from that of entire genes. The GC content of codons 2-6 is significantly reduced compared with that of the remainder of the gene. The possible implications of a reduced GC content for the control of translation efficiency are discussed.

Pouwels, P H; Leunissen, J A

1994-01-01

64

Comparison of fecundity and offspring immunity in zebrafish fed Lactobacillus rhamnosus CICC 6141 and Lactobacillus casei BL23.  

PubMed

To increase the knowledge of probiotic effects on zebrafish (Danio rerio), we compare the effects of two probiotic strains, Lactobacillus rhamnosus CICC 6141 (a highly adhesive strain) and Lactobacillus casei BL23 (a weakly adhesive strain), on zebrafish reproduction and their offsprings' innate level of immunity to water-borne pathogens. During probiotics treatments from 7 to 28 days, both the Lactobacillus strains, and especially L. casei BL23, significantly increased fecundity in zebrafish: higher rates of egg ovulation, fertilization, and hatching were observed. Increased densities of both small and large vitellogenic follicles, seen in specimens fed either Lactobacillus strain, demonstrated accelerated oocyte maturation. Feeding either strain of Lactobacillus upregulated gene expression of leptin, kiss2, gnrh3, fsh, lh, lhcgr, and paqr8, which were regarded to enhance fecundity and encourage oocyte maturation. Concomitantly, the gene expression of bmp15 and tgfb1 was inhibited, which code for local factors that prevent oocyte maturation. The beneficial effects of the Lactobacillus strains on fecundity diminished after feeding of the probiotics was discontinued, even for the highly adhesive gut Lactobacillus strain. Administering L. rhamnosus CICC 6141 for 28 days was found to affect the innate immunity of offspring derived from their parents, as evinced by a lower level of alkaline phosphatase activity in early larval stages. This study highlights the effects of probiotics both upon the reproductive process and upon the offsprings' immunity during early developmental stages. PMID:24129154

Qin, Chubin; Xu, Li; Yang, Yalin; He, Suxu; Dai, Yingying; Zhao, Huiying; Zhou, Zhigang

2014-01-01

65

Homodimeric ?-Galactosidase from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081: Expression in Lactobacillus plantarum and Biochemical Characterization  

PubMed Central

The lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081, encoding a ?-galactosidase of the glycoside hydrolase family GH2, was cloned into different inducible lactobacillal expression vectors for overexpression in the host strain Lactobacillus plantarum WCFS1. High expression levels were obtained in laboratory cultivations with yields of approximately 53000 U of ?-galactosidase activity per liter of medium, which corresponds to ?170 mg of recombinant protein per liter and ?-galactosidase levels amounting to 63% of the total intracellular protein of the host organism. The wild-type (nontagged) and histidine-tagged recombinant enzymes were purified to electrophoretic homogeneity and further characterized. ?-Galactosidase from L. bulgaricus was used for lactose conversion and showed very high transgalactosylation activity. The maximum yield of galacto-oligosaccharides (GalOS) was approximately 50% when using an initial concentration of 600 mM lactose, indicating that the enzyme can be of interest for the production of GalOS.

2012-01-01

66

Dominance of Lactobacillus acidophilus in the facultative jejunal Lactobacillus microbiota of fistulated beagles.  

PubMed

Lactobacilli were isolated from jejunal chyme from five fistulated beagles. Cultivable lactobacilli varied from 10(4) to 10(8) CFU/ml. Seventy-four isolates were identified by partial 16S rRNA gene sequencing and differentiated by repetitive element PCR (Rep-PCR), Lactobacillus acidophilus was dominant, and nearly 80% of 54 isolates shared the same DNA fingerprint pattern. PMID:22843523

Tang, Yurui; Manninen, Titta J K; Saris, Per E J

2012-10-01

67

Isolation and characterization of exocellular polysaccharide produced by Lactobacillus bulgaricus  

Microsoft Academic Search

Summary Lactobacillus bulgaricus strains grown on skim milk produce a viscosifying exocellular watersoluble heteropolysaccharide composed of galactose, glucose and rhamnose in an approximately molar ratio of 4:1:1. The molecular weight is approximately 500.000.

Jutta Cerning; C. Bouillanne; M. J. Desmazeaud; Michèle Landon

1986-01-01

68

Draft Genome Sequence of Lactobacillus animalis 381-IL-28.  

PubMed

Lactobacillus animalis 381-IL-28 is an integral component of a multistrain commercial culture with food biopreservative and pathogen biocontrol functionality. A draft sequence of the L. animalis 381-IL-28 genome is described in this paper. PMID:24874675

Sturino, Joseph M; Rajendran, Mahitha; Altermann, Eric

2014-01-01

69

Distribution of genital Lactobacillus strains shared by female sex partners.  

PubMed

The prevalence of bacterial vaginosis among lesbians is high. We assessed whether unique Lactobacillus strains were shared by female sex partners. Cultures of vaginal and rectal specimens for detection of Lactobacillus organisms were performed for women who reported having had sex with women. Lactobacilli were identified on the basis of DNA homology and were typed and fingerprinted by repetitive element sequence-based polymerase chain reaction (rep-PCR). Of 237 women, Lactobacillus crispatus was detected in 98% and Lactobacillus gasseri in 21%. Detection of L. gasseri was associated with recent receptive digital-vaginal sex (P = .04) and increased bacterial vaginosis risk (odds ratio, 4.2; 95% confidence interval, 1.4-13.4). Of 31 couples monogamous for >3 months, rep-PCR fingerprints were identical in both members in 23 (77%). PMID:19199538

Marrazzo, Jeanne M; Antonio, May; Agnew, Kathy; Hillier, Sharon L

2009-03-01

70

Draft Genome Sequence of Lactobacillus animalis 381-IL-28  

PubMed Central

Lactobacillus animalis 381-IL-28 is an integral component of a multistrain commercial culture with food biopreservative and pathogen biocontrol functionality. A draft sequence of the L. animalis 381-IL-28 genome is described in this paper.

Rajendran, Mahitha; Altermann, Eric

2014-01-01

71

Electron Micrograph of the Meat Spoilage Bacterium Lactobacillus sake  

NSDL National Science Digital Library

This image is of the meat spoilage bacterium Lactobacillus sake. This strain was originally isolated from a vacuum-packaged meat product. To enhance its aesthetic appeal, the original black and white image was colored using Adobe Photoshop.

American Society For Microbiology;

2003-09-22

72

Genome Sequence of Lactobacillus plantarum Strain UCMA 3037  

PubMed Central

Nucleic acid of the strain Lactobacillus plantarum UCMA 3037, isolated from raw milk camembert cheese in our laboratory, was sequenced. We present its draft genome sequence with the aim of studying its functional properties and relationship to the cheese ecosystem.

Naz, Saima; Tareb, Raouf; Bernardeau, Marion; Vaisse, Melissa; Lucchetti-Miganeh, Celine; Rechenmann, Mathias

2013-01-01

73

Viricidal Effects of Lactobacillus and Yeast Fermentation  

PubMed Central

The survival of selected viruses in Lactobacillus- and yeast-fermented edible waste material was studied to determine the feasibility of using this material as a livestock feed ingredient. Five viruses, including Newcastle disease virus, infectious canine hepatitis virus, a porcine picornavirus, frog virus 3, and bovine virus diarrhea, were inoculated into a mixture of ground food waste (collected from a school lunch program) containing Lactobacillus acidophilus. Mixtures were incubated at 20, 30, and 40°C for 216 h. In a second trial, four viruses, including Newcastle disease virus, infectious canine hepatitis virus, frog virus 3, and a porcine picornavirus, were inoculated into similar edible waste material containing Saccharomyces cerevisiae. Mixtures were incubated at 20 and 30°C for 216 h. Samples were obtained daily for quantitative (trial 1) and qualitative (trial 2) virus isolation. Temperature, pH, and redox potential were monitored. Controlled pH and temperature studies were also done and compared with the inactivation rates in the fermentation processes. In trial 1 (Lactobacillus fermentation), infectious canine hepatitis virus survived the entire test period in the fermentation process but was inactivated below pH 4.5 in the controlled studies. Newcastle disease virus was inactivated by day 8 in the fermentation process and appeared to be primarily heat sensitive and secondarily pH sensitive in the controlled studies. The porcine picornavirus survived the fermentation process for 8 days at 20°C but was inactivated more rapidly at 30 and 40°C. The controlled studies verified these findings. Frog virus 3 was inactivated by day 3 in the fermentation process and appeared to be sensitive to low pH in the controlled studies. Bovine virus diarrhea was rapidly inactivated in the fermentation process (less than 2 h) and was pH and temperature sensitive. In trial 2 (yeast fermentation), infectious hepatitis virus survived the entire test period in the fermentation process. Newcastle disease virus was inactivated by day 7 at 20°C and day 6 at 30°C. The porcine picornavirus was inactivated by day 7 at 30°C but survived the entire test period at 20°C. Frog virus 3 was inactivated by day 3 at 20°C and day 2 at 30°C.

Gilbert, Jeannine P.; Wooley, Richard E.; Shotts, Emmett B.; Dickens, J. Andra

1983-01-01

74

Effects of feeding lactobacillus GG on lethal irradiation in mice  

SciTech Connect

Mice exposed to 1400 rads of total body irradiation experienced 80%-100% mortality in 2 wk. Bacteremia was demonstrated in all dead animals. Feeding Lactobacillus GG strain reduced Pseudomonas bacteremia and prolonged survival time in animals colonized with this organism. In animals not colonized with Pseudomonas, feeding Lactobacillus GG also produced some reduction in early deaths, and there was less Gram-negative bacteremia in these animals compared with controls.

Dong, M.Y.; Chang, T.W.; Gorbach, S.L.

1987-05-01

75

Surface Display of Foreign Epitopes on the Lactobacillus brevis SLayer  

Microsoft Academic Search

So far, the inability to establish viable Lactobacillus surface layer (S-layer) null mutants has hampered the biotechnological applications of Lactobacillus S-layers. In this study, we demonstrate the utilization of Lacto- bacillus brevis S-layer subunits (SlpA) for the surface display of foreign antigenic epitopes. With an inducible expression system, L. brevis strains producing chimeric S-layers were obtained after testing of four

S. Avall-Jaaskelainen; K. Kyla-Nikkila; Minna Kahala; Terhi Miikkulainen-Lahti; Airi Palva

2002-01-01

76

Lactobacillus shenzhenensis sp. nov., isolated from a fermented dairy beverage.  

PubMed

Two Lactobacillus strains, designated LY-73(T) and LY-30B, were isolated from a dairy beverage, sold in Shenzhen market, China. The two isolates were Gram-positive, non-spore-forming, non-motile, facultatively anaerobic rods that were heterofermentative and did not exhibit catalase activity. Sequencing of the 16S rRNA, pheS and rpoA genes revealed that the two isolates shared 99.5, 99.8 and 99.9?% sequence similarity, which indicates that they belong to the same species. Phylogenetic analysis demonstrated clustering of the two isolates with the genus Lactobacillus. Strain LY-73(T) showed highest 16S rRNA gene sequence similarities with Lactobacillus harbinensis KACC 12409(T) (97.73%), Lactobacillus perolens DSM 12744(T) (96.96?%) and Lactobacillus selangorensis DSM 13344(T) (93.10?%). Comparative analyses of their rpoA and pheS gene sequences indicated that the novel strains were significantly different from other Lactobacillus species. Low DNA-DNA reassociation values (50.5?%) were obtained between strain LY-73(T) and its phylogenetically closest neighbours. The G+C contents of the DNA of the two novel isolates were 56.1 and 56.5 mol%. Straight-chain unsaturated fatty acids C18?:?1?9c (78.85 and 74.29?%) were the dominant components, and the cell-wall peptidoglycan was of the l-Lys-d-Asp type. Based on phenotypic characteristics, and chemotaxonomic and genotypic data, the novel strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus shenzhenensis sp. nov. is proposed, with LY-73(T) (?=?CCTCC M 2011481(T)?=?KACC 16878(T)) as the type strain. PMID:23002044

Zou, Yuanqiang; Liu, Feng; Fang, Chengxiang; Wan, Daiwei; Yang, Rentao; Su, Qingqing; Yang, Ruifu; Zhao, Jiao

2013-05-01

77

Lactobacillus faecis sp. nov., isolated from animal faeces.  

PubMed

Three lactic acid bacteria were isolated from faeces of a jackal (Canis mesomelas) and raccoons (Procyron lotor). The isolates formed a subcluster in the Lactobacillus salivarius phylogenetic group, closely related to Lactobacillus animalis, Lactobacillus apodemi and Lactobacillus murinus, by phylogenetic analysis based on 16S rRNA and recA gene sequences. Levels of DNA-DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. The three strains were non-motile, obligately homofermentative and produced l-lactic acid as the main end-product from d-glucose. The strains metabolized raffinose. The major cellular fatty acids in the three strains were C16?:?0, C18?:?1?9c and C19?:?1 cyclo 9,10. Based on the data provided, it is concluded that the three strains represent a novel species of the genus Lactobacillus, for which the name Lactobacillus faecis sp. nov. is proposed. The type strain is AFL13-2(T) (?=?JCM 17300(T)?=?DSM 23956(T)). PMID:23907223

Endo, Akihito; Irisawa, Tomohiro; Futagawa-Endo, Yuka; Salminen, Seppo; Ohkuma, Moriya; Dicks, Leon

2013-12-01

78

Testing of viscous anti-HIV microbicides using Lactobacillus.  

PubMed

The development of topical microbicides for intravaginal use to prevent HIV infection requires that the drugs and formulated products be nontoxic to the endogenous vaginal Lactobacillus. In 30min exposure tests we found dapivirine, tenofovir and UC781 (reverse transcriptase inhibitor anti-HIV drugs) as pure drugs or formulated as film or gel products were not deleterious to Lactobacillus species; however, PSC-RANTES (a synthetic CCR5 antagonist) killed 2 strains of Lactobacillus jensenii. To demonstrate the toxicity of formulated products a new assay was developed for use with viscous and non-viscous samples that we have termed the Lactobacillus toxicity test. We found that the vortex mixing of vaginal Lactobacillus species can lead to reductions in bacterial viability. Lactobacillus can survive briefly, about 2s, but viability declines with increased vortex mixing. The addition of heat inactivated serum or bovine serum albumin, but not glycerol, prevented the decrease in bacterial viability. Bacillus atrophaeus spores also demonstrated loss of viability upon extended mixing. We observed that many of the excipients used in film formulation and the films themselves also afford protection from the killing during vortex mixing. This method is of relevance for toxicity for cidal activities of viscous products. PMID:22226641

Moncla, B J; Pryke, K; Rohan, L C; Yang, H

2012-02-01

79

Molecular identification of Lactobacillus spp. associated with puba, a Brazilian fermented cassava food  

PubMed Central

Puba or carimã is a Brazilian staple food obtained by spontaneous submerged fermentation of cassava roots. A total of 116 lactobacilli and three cocci isolates from 20 commercial puba samples were recovered on de Man, Rogosa and Sharpe agar (MRS); they were characterized for their antagonistic activity against foodborne pathogens and identified taxonomically by classical and molecular methods. In all samples, lactic acid bacteria were recovered as the dominant microbiota (7.86 ± 0.41 log10 CFU/g). 16S–23S rRNA ARDRA pattern assigned 116 isolates to the Lactobacillus genus, represented by the species Lactobacillus fermentum (59 isolates), Lactobacillus delbrueckii (18 isolates), Lactobacillus casei (9 isolates), Lactobacillus reuteri (6 isolates), Lactobacillus brevis (3 isolates), Lactobacillus gasseri (2 isolates), Lactobacillus nagelii (1 isolate), and Lactobacillus plantarum group (18 isolates). recA gene-multiplex PCR analysis revealed that L. plantarum group isolates belonged to Lactobacillus plantarum (15 isolates) and Lactobacillus paraplantarum (3 isolates). Genomic diversity was investigated by molecular typing with rep (repetitive sequence)-based PCR using the primer ERIC2 (enterobacterial repetitive intergenic consensus). The Lactobacillus isolates exhibited genetic heterogeneity and species-specific fingerprint patterns. All the isolates showed antagonistic activity against the foodborne pathogenic bacteria tested. This antibacterial effect was attributed to acid production, except in the cases of three isolates that apparently produced bacteriocin-like inhibitory substances. This study provides the first insight into the genetic diversity of Lactobacillus spp. of puba.

Crispim, S.M.; Nascimento, A.M.A.; Costa, P.S.; Moreira, J.L.S.; Nunes, A.C.; Nicoli, J.R.; Lima, F.L.; Mota, V.T.; Nardi, R.M.D.

2013-01-01

80

Genomic adaptation of the Lactobacillus casei group.  

PubMed

Lactobacillus casei, L. paracasei, and L. rhamnosus form a closely related taxonomic group (Lactobacillus casei group) within the facultatively heterofermentative lactobacilli. Here, we report the complete genome sequences of L. paracasei JCM 8130 and L. casei ATCC 393, and the draft genome sequence of L. paracasei COM0101, all of which were isolated from daily products. Furthermore, we re-annotated the genome of L. rhamnosus ATCC 53103 (also known as L. rhamnosus GG), which we have previously reported. We confirmed that ATCC 393 is distinct from other strains previously described as L. paracasei. The core genome of 10 completely sequenced strains of the L. casei group comprised 1,682 protein-coding genes. Although extensive genome-wide synteny was found among the L. casei group, the genomes of ATCC 53103, JCM 8130, and ATCC 393 contained genomic islands compared with L. paracasei ATCC 334. Several genomic islands, including carbohydrate utilization gene clusters, were found at the same loci in the chromosomes of the L. casei group. The spaCBA pilus gene cluster, which was first identified in GG, was also found in other strains of the L. casei group, but several L. paracasei strains including COM0101 contained truncated spaC gene. ATCC 53103 encoded a higher number of proteins involved in carbohydrate utilization compared with intestinal lactobacilli, and extracellular adhesion proteins, several of which are absent in other strains of the L. casei group. In addition to previously fully sequenced L. rhamnosus and L. paracasei strains, the complete genome sequences of L. casei will provide valuable insights into the evolution of the L. casei group. PMID:24116025

Toh, Hidehiro; Oshima, Kenshiro; Nakano, Akiyo; Takahata, Muneaki; Murakami, Masaru; Takaki, Takashi; Nishiyama, Hidetoshi; Igimi, Shizunobu; Hattori, Masahira; Morita, Hidetoshi

2013-01-01

81

Cell-free supernatants from probiotic Lactobacillus casei and Lactobacillus rhamnosus GG decrease colon cancer cell invasion in vitro.  

PubMed

Probiotics have been shown to have a preventative role in colorectal carcinogenesis but research concerning their prophylactic potential in the later stages of colorectal cancer, specifically metastasis is limited. This study explored the potential of cell-free supernatants (CFS) from 2 probiotic Lactobacillus sp., Lactobacillus casei and Lactobacillus rhamnosus GG, to inhibit colon cancer cell invasion by influencing matrix metalloproteinase-9 (MMP-9) activity and levels of the tight junction protein zona occludens-1 (ZO-1) in cultured metastatic human colorectal carcinoma cells. HCT-116 cells were treated with CFS from L. casei, L. rhamnosus, or Bacteroides thetaiotaomicron (a gut commensal); or with uninoculated bacterial growth media. Treatment with CFS from both Lactobacillus sp. decreased colorectal cell invasion but treatment with CFS from B. thetaiotaomicron did not. CFS from both Lactobacillus sp. decreased MMP-9 and increased ZO-1 protein levels. L. rhamnosus CFS also lowered MMP-9 activity. To begin elucidating the secreted bacterial factor conveying these responses, Lactobacillus sp. CFS were fractionated into defined molecular weight ranges and cell invasion assessed. Fractionation revealed that the inhibitory activity was contained primarily in the >100 kDa and 50-100 kDa fractions, suggesting the inhibitory compound may be a macromolecule such as a protein, nucleic acid, or a polysaccharide. PMID:22830611

Escamilla, Juanita; Lane, Michelle A; Maitin, Vatsala

2012-08-01

82

Purification of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced  

Microsoft Academic Search

Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme

Kees Bonting; Marc J. E. C. van der Maarel; Lubbert Dijkhuizen

2001-01-01

83

Molecular analysis and clinical significance of Lactobacillus spp. recovered from clinical specimens presumptively associated with disease.  

PubMed

Lactobacillus spp. are part of the normal human flora and are generally assumed to be nonpathogenic. We determined the genotypic identification of >100 Lactobacillus isolates from clinical specimens in the context of presumed pathogenic potential (e.g., recovered as the single/predominant isolate from a sterile site or at ?10(5) CFU/ml from urine). This study assessed the clinical significance and the frequency of occurrence of each Lactobacillus sp. We identified 16 species of Lactobacillus by 16S rRNA gene sequence analysis, 10 of which could not be associated with disease. While Lactobacillus rhamnosus, Lactobacillus gasseri, and Lactobacillus paracasei were associated with infections, L. gasseri was also a common colonizing/contaminating species. Lactobacillus casei, Lactobacillus johnsonii, and Lactobacillus delbrueckii were associated with at least one infection. Species commonly used in probiotic products (e.g., L. rhamnosus and L. casei) were identical, by 16S rRNA gene sequencing, to our isolates associated with disease. Human isolates of Lactobacillus spp. have differing site associations and levels of clinical significance. Knowing the niche and pathogenic potential of each Lactobacillus sp. can be of importance to both clinical microbiology and the food and probiotic supplement industry. PMID:24131686

Martinez, Raquel M; Hulten, Kristina G; Bui, Uyen; Clarridge, Jill E

2014-01-01

84

Growth phase of orally administered Lactobacillus strains differentially affects T helper-cell pathways  

Microsoft Academic Search

Lactobacillus strains with probiotic activity are major constituents of numerous common food products. Due to their `generally regarded as safe¿-status (GRAS-status), Lactobacillus strains can also be genetically engineered for use in oral immunotherapeutic applications, such as vaccination and T lymphocyte tolerance induction in autoimmune disease. In the current study, we demonstrate that the growth phase of orally administered individual Lactobacillus

C. B. M. Maassen; W. J. A. Boersma; Holten-Neelen van J. C. P. A; E. A. W. Claassen; J. D. Laman

2003-01-01

85

Molecular Analysis and Clinical Significance of Lactobacillus spp. Recovered from Clinical Specimens Presumptively Associated with Disease  

PubMed Central

Lactobacillus spp. are part of the normal human flora and are generally assumed to be nonpathogenic. We determined the genotypic identification of >100 Lactobacillus isolates from clinical specimens in the context of presumed pathogenic potential (e.g., recovered as the single/predominant isolate from a sterile site or at ?105 CFU/ml from urine). This study assessed the clinical significance and the frequency of occurrence of each Lactobacillus sp. We identified 16 species of Lactobacillus by 16S rRNA gene sequence analysis, 10 of which could not be associated with disease. While Lactobacillus rhamnosus, Lactobacillus gasseri, and Lactobacillus paracasei were associated with infections, L. gasseri was also a common colonizing/contaminating species. Lactobacillus casei, Lactobacillus johnsonii, and Lactobacillus delbrueckii were associated with at least one infection. Species commonly used in probiotic products (e.g., L. rhamnosus and L. casei) were identical, by 16S rRNA gene sequencing, to our isolates associated with disease. Human isolates of Lactobacillus spp. have differing site associations and levels of clinical significance. Knowing the niche and pathogenic potential of each Lactobacillus sp. can be of importance to both clinical microbiology and the food and probiotic supplement industry.

Martinez, Raquel M.; Hulten, Kristina G.; Bui, Uyen

2014-01-01

86

Distribution Dynamics of Recombinant Lactobacillus in the Gastrointestinal Tract of Neonatal Rats  

PubMed Central

One approach to deliver therapeutic agents, especially proteins, to the gastro-intestinal (GI) tract is to use commensal bacteria as a carrier. Genus Lactobacillus is an attractive candidate for use in this approach. However, a system for expressing exogenous proteins at a high level has been lacking in Lactobacillus. Moreover, it will be necessary to introduce the recombinant Lactobacillus into the GI tract, ideally by oral administration. Whether orally administered Lactobacillus can reach and reside in the GI tract has not been explored in neonates. In this study, we have examined these issues in neonatal rats. To achieve a high level of protein expression in Lactobacillus, we tested the impact of three promoters and two backbones on protein expression levels using mRFP1, a red fluorescent protein, as a reporter. We found that a combination of an L-lactate dehydrogenase (ldhL) promoter of Lactobacillus sakei with a backbone from pLEM415 yielded the highest level of reporter expression. When this construct was used to transform Lactobacillus casei, Lactobacillus delbrueckii and Lactobacillus acidophilus, high levels of mRFP1 were detected in all these species and colonies of transformed Lactobacillus appeared pink under visible light. To test whether orally administered Lactobacillus can be retained in the GI tract of neonates, we fed the recombinant Lactobacillus casei to neonatal rats. We found that about 3% of the bacteria were retained in the GI tract of the rats at 24 h after oral feeding with more recombinant Lactobacillus in the stomach and small intestine than in the cecum and colon. No mortality was observed throughout this study with Lactobacillus. In contrast, all neonatal rats died within 24 hours after fed with transformed E. coli. Taken together, our results indicate that Lactobacillus has the potential to be used as a vehicle for the delivery of therapeutic agents to neonates.

Bao, Sujin; Zhu, Libin; Zhuang, Qiang; Wang, Lucia; Xu, Pin-Xian; Itoh, Keiji; Holzman, Ian R.; Lin, Jing

2013-01-01

87

Decolorization of water and oil-soluble azo dyes by Lactobacillus acidophilus and Lactobacillus fermentum.  

PubMed

The capability of Lactobacillus acidophilus and Lactobacillus fermentum to degrade azo dyes was investigated. The bacteria were incubated under anaerobic conditions in the presence of 6 microg/ml Methyl Red, Ponceau BS, Orange G, Amaranth, Orange II, and Direct Blue 15; 5 microg/ml Sudan I and II; or 1.5 microg/ml Sudan III and IV in deMann-Rogosa-Sharpe broth at 37 degrees C for 36 h, and reduction of the dyes was monitored. Both bacteria were capable of degrading all of the water-soluble azo dyes to some extent. They were also able to completely reduce the oil-soluble diazo dyes Sudan III and IV but were unable to reduce the oil-soluble monoazo dyes Sudan I and II to any significant degree in the concentrations studied. Growth of the bacteria was not significantly affected by the presence of the Sudan azo dyes. Metabolites of the bacterial degradation of Sudan III and IV were isolated and identified by liquid chromatography electrospray ionization tandem mass spectrometry analyses and compared with authentic standards. Aniline and o-toluidine (2-methylaniline), both potentially carcinogenic aromatic amines, were metabolites of Sudan III and IV, respectively. PMID:19727875

Chen, Huizhong; Xu, Haiyan; Heinze, Thomas M; Cerniglia, Carl E

2009-12-01

88

Hydrolysis of Pork Muscle Sarcoplasmic Proteins by Lactobacillus curvatus and Lactobacillus sake  

PubMed Central

Lactobacillus curvatus CECT 904 and Lactobacillus sake CECT 4808 were selected on the basis of their proteolytic activities against synthetic substrates. Further, the effects of whole cells, cell extracts, and a combination of both enzymatic sources on muscle sarcoplasmic proteins were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reverse-phase high-performance liquid chromatography analyses. Strains of both species displayed proteinase activities on five sarcoplasmic proteins. The inoculation of whole cells caused a degradation of peptides, whereas the addition of cell extracts resulted in the generation of both hydrophilic and hydrophobic peptides. This phenomenon was remarkably more pronounced when L. curvatus was involved. Whole cells also consumed a great amount of free amino acids, while the addition of intracellular enzymes contributed to their generation. L. sake accounted for a greater release of free amino acids. In general, cell viability and also proteolytic events were promoted when cell suspensions were provided with cell extracts as an extra source of enzymes.

Fadda, Silvina; Sanz, Yolanda; Vignolo, Graciela; Aristoy, M.-Concepcion; Oliver, Guillermo; Toldra, Fidel

1999-01-01

89

The characterization of undecaprenol of Lactobacillus plantarum  

PubMed Central

Evidence for the presence of undecaprenol in the unsaponifiable lipid of Lactobacillus plantarum (N.C.I.B. 6376) is presented. Characterization of the compound was based mainly on mass, i.r. and n.m.r. spectrometry. The prenol was isolated at a concentration of 40?g/g wet wt. of bacteria and contained over 90% (1.0–5.4% of the dose) of the 14C present in the unsaponifiable lipid after incubation of the bacteria with [2-14C]mevalonate. N.m.r. spectrometry indicated the presence of two internal trans-, one ?-cis- and seven internal cis-isoprene residues per molecule. The 3H/14C ratios of the prenol after incubation of the bacteria with [2-14C,(4R)-4-3H1]- and [2-14C,(4S)-4-3H1]-mevalonate were in agreement with this stereochemistry. There was no evidence of saturated isoprene residues in the molecule. The undecaprenol appeared to be accompanied by much smaller quantities of decaprenol and nonaprenol.

Gough, Doreen P.; Kirby, Alison L.; Richards, J. B.; Hemming, F. W.

1970-01-01

90

Genome instability in Lactobacillus rhamnosus GG.  

PubMed

We describe here a comparative genome analysis of three dairy product isolates of Lactobacillus rhamnosus GG (LGG) and the ATCC 53103 reference strain to the published genome sequence of L. rhamnosus GG. The analysis showed that in two of three isolates, major DNA segments were missing from the genomic islands LGGISL1,2. The deleted DNA segments consist of 34 genes in one isolate and 84 genes in the other and are flanked by identical insertion elements. Among the missing genes are the spaCBA genes, which encode pilin subunits involved in adhesion to mucus and persistence of the strains in the human intestinal tract. Subsequent quantitative PCR analyses of six commercial probiotic products confirmed that two more products contain a heterogeneous population of L. rhamnosus GG variants, including genotypes with or without spaC. These results underline the relevance for quality assurance and control measures targeting genome stability in probiotic strains and justify research assessing the effect of genetic rearrangements in probiotics on the outcome of in vitro and in vivo efficacy studies. PMID:23354703

Sybesma, Wilbert; Molenaar, Douwe; van IJcken, Wilfred; Venema, Koen; Kort, Remco

2013-04-01

91

Genome Instability in Lactobacillus rhamnosus GG  

PubMed Central

We describe here a comparative genome analysis of three dairy product isolates of Lactobacillus rhamnosus GG (LGG) and the ATCC 53103 reference strain to the published genome sequence of L. rhamnosus GG. The analysis showed that in two of three isolates, major DNA segments were missing from the genomic islands LGGISL1,2. The deleted DNA segments consist of 34 genes in one isolate and 84 genes in the other and are flanked by identical insertion elements. Among the missing genes are the spaCBA genes, which encode pilin subunits involved in adhesion to mucus and persistence of the strains in the human intestinal tract. Subsequent quantitative PCR analyses of six commercial probiotic products confirmed that two more products contain a heterogeneous population of L. rhamnosus GG variants, including genotypes with or without spaC. These results underline the relevance for quality assurance and control measures targeting genome stability in probiotic strains and justify research assessing the effect of genetic rearrangements in probiotics on the outcome of in vitro and in vivo efficacy studies.

Molenaar, Douwe; van IJcken, Wilfred; Venema, Koen

2013-01-01

92

Lactobacillus bulgaricus mutants decompose uremic toxins.  

PubMed

Abstract Background/Aims: We aim to obtain a probiotic strain from Lactobacillus bulgaricus by testing its capability to decompose uremic toxins to provide new intestinal bacteria for the treatment of chronic renal failure. Methods: Original L. bulgaricus was cultured with the serum of uremic patients and then mutated by physical (ultraviolet) and chemical (diethyl sulfate) methods repeatedly. Using creatinine decomposition rate as an observed index, we selected the best strains which decreased the most concentration of the creatinine. We then tested its ability to decompose urea, uric acid, serum phosphate, parathyroid hormone, and homocysteine and its genetic stability. Results: After inductive and mutagenic treatment, DUC3-17 was selected. Its decomposition rate of creatinine, urea nitrogen, uric acid, phosphorus, parathyroid hormone, and homocysteine were 17.23%, 36.02%, 9.84%, 15.73%, 78.26%, and 12.69%, respectively. The degrading capacity was sustained over five generations. Conclusions: After directional induction and compound mutation, L. bulgaricus has greater capacity to decompose uremic toxins, with a stable inheritance. PMID:24575979

Bai, Yun-Huan; Jiang, Ya-Fen; Jiang, Yun-Sheng

2014-06-01

93

Probiotic features of two oral Lactobacillus isolates.  

PubMed

In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics. PMID:24031847

Zavisic, Gordana; Petricevic, Sasa; Radulovic, Zeljka; Begovic, Jelena; Golic, Natasa; Topisirovic, Ljubisa; Strahinic, Ivana

2012-01-01

94

Probiotic features of two oral Lactobacillus isolates  

PubMed Central

In this study, we checked lactobacilli strains of human origin for their potential as probiotic. Samples were collected from oral mucosa of 16 healthy individuals, out of which twenty isolates were obtained and two of them were selected and identified as Lactobacillus plantarum (G1) and L. casei (G3). Both isolates exhibited antagonistic action towards pathogenic microorganisms such as Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella abony, and Clostridium sporogenes, but not on the growth of Candida albicans. The bacteriocin activity against Staphylococcus aureus ATCC 6358-P was shown only by L. plantarum G1. Moreover, the isolates G1 and G3 showed good viability in the acid gastric environment and in the gut environment containing bovine bile salts. The viability of G1 and G3 isolates in the gastrointestinal tract, and the adhesion to the intestinal mucosa were also confirmed in vivo. The biochemical tests of blood samples revealed lower levels of serum triglycerides and cholesterol, as well as reduced activity of alkaline phosphatase in all lactobacilli-treated Wistar rats, compared to control ones. No toxicity for NMRI Ham mice was observed. According to our experimental results, these findings imply that L. plantarum G1 and L. casei G3 could be characterized as potential probiotics.

Zavisic, Gordana; Petricevic, Sasa; Radulovic, Zeljka; Begovic, Jelena; Golic, Natasa; Topisirovic, Ljubisa; Strahinic, Ivana

2012-01-01

95

Acute acalculous cholecystitis complicated with peritonitis caused by Lactobacillus plantarum.  

PubMed

Lactobacillus spp. rarely causes human disease. We report a case of a 57-year-old man with non-insulin-dependent diabetes and vascular disease admitted to our hospital with severe abdominal pain and fever. Signs of peritonitis were found upon examination. The patient underwent surgery, and a diagnosis of perforated cholecystitis with purulent peritonitis was made intra-operatively. A cholecystectomy was performed, and therapy with imipenem was initiated. Lactobacillus plantarum was isolated from bile and peritoneal fluid cultures 2 days later. The patient recovered well and was discharged on post-operative day 16 after 14 days of treatment with imipenem. To our knowledge, this is the second case reported of acute cholecystitis caused by Lactobacillus spp. This organism should be considered as a cause of biliary infections, especially in patients with underlying diseases. Correct identification is often difficult, but it is very important because these organisms are usually resistant to vancomycin and other antibiotics. PMID:23886436

Tena, Daniel; Martínez, Nora Mariela; Losa, Cristina; Fernández, Cristina; Medina, María José; Sáez-Nieto, Juan Antonio

2013-08-01

96

Lactobacillus pobuzihii sp. nov., isolated from pobuzihi (fermented cummingcordia).  

PubMed

Twenty-one homofermentative lactic acid bacteria were isolated from fermented cummingcordia (pobuzihi), a traditional food in Taiwan. The isolates had identical 16S rRNA gene sequences that were distinct from those of other lactobacilli, and their closest neighbours in the 16S rRNA gene sequence phylogenetic tree were strains of Lactobacillus acidipiscis. Levels of DNA-DNA relatedness between representative pobuzihi isolates and strains of L. acidipiscis were 17% and below. Furthermore, the new isolates could be differentiated clearly from L. acidipiscis NBRC 102163T and NBRC 102164 in terms of acid production from L-arabinose, rhamnose, mannitol, lactose and 5-ketogluconate. It was concluded that the new isolates represent a single novel species of the genus Lactobacillus, for which the name Lactobacillus pobuzihii sp. nov. is proposed. The type strain is E100301T (=RIFY 6501T =NBRC 103219T =KCTC 13174T). PMID:19783610

Chen, Yi-Sheng; Miyashita, Mika; Suzuki, Ken-ichiro; Sato, Hajime; Hsu, Jar-Sheng; Yanagida, Fujitoshi

2010-08-01

97

Quantitative transcript analysis of the inducible expression system pSIP: comparison of the overexpression of Lactobacillus spp. ?-galactosidases in Lactobacillus plantarum  

Microsoft Academic Search

Background  Two sets of overlapping genes, lacLMReu and lacLMAci, encoding heterodimeric ?-galactosidases from Lactobacillus reuteri and Lactobacillus acidophilus, respectively, have previously been cloned and expressed using the pSIP vector system and Lactobacillus plantarum WCSF1 as host. Despite the high similarity between these lacLM genes and the use of identical cloning and expression strategies, strains harboring lacLMReu produced about twenty-fold more ?-galactosidase

Tien-Thanh Nguyen; Thu-Ha Nguyen; Thomas Maischberger; Philipp Schmelzer; Geir Mathiesen; Vincent GH Eijsink; Dietmar Haltrich; Clemens K Peterbauer

2011-01-01

98

Lactobacillus cypricasei sp. nov., isolated from Halloumi cheese.  

PubMed

Four strains of a hitherto unknown bacterium isolated from Halloumi cheese were compared by using phenotypic and phylogenetic studies. Comparative 16S rRNA gene sequencing demonstrated that the strains were identical to each other and represent a new subline within the genus Lactobacillus. The unknown bacterium was readily distinguished from other described Gram-positive catalase-negative taxa by means of biochemical tests and electrophoretic analysis of whole-cell proteins. On the basis of phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Lactobacillus cypricasei sp. nov. The type strain of L. cypricasei is CCUG 42961T (= CIP 106393T). PMID:11211271

Lawson, P A; Papademas, P; Wacher, C; Falsen, E; Robinson, R; Collins, M D

2001-01-01

99

Crystal structure of tannase from Lactobacillus plantarum.  

PubMed

Tannins are water-soluble polyphenolic compounds in plants. Hydrolyzable tannins are derivatives of gallic acid (3,4,5-trihydroxybenzoic acid) or its meta-depsidic forms that are esterified to polyol, catechin, or triterpenoid units. Tannases are a family of esterases that catalyze the hydrolysis of the galloyl ester bond in hydrolyzable tannins to release gallic acid. The enzymes have found wide applications in food, feed, beverage, pharmaceutical, and chemical industries since their discovery more than a century ago, although little is known about them at the molecular level, including the details of the catalytic and substrate binding sites. Here, we report the first three-dimensional structure of a tannase from Lactobacillus plantarum. The enzyme displays an ?/? structure, featured by a large cap domain inserted into the classical serine hydrolase fold. A catalytic triad was identified in the structure, which is composed of Ser163, His451, and Asp419. During the binding of gallic acid, the carboxyl group of the molecule forges hydrogen-bonding interactions with the catalytic triad of the enzyme while the three hydroxyl groups make contacts with Asp421, Lys343, and Glu357 to form another hydrogen-bonding network. Mutagenesis studies demonstrated that these residues are indispensable for the activity of the enzyme. Structural studies of the enzyme in complex with a number of substrates indicated that the interactions at the galloyl binding site are the determinant force for the binding of substrates. The single galloyl binding site is responsible for the esterase and depsidase activities of the enzyme. PMID:23648840

Ren, Bin; Wu, Mingbo; Wang, Qin; Peng, Xiaohong; Wen, Hua; McKinstry, William J; Chen, Qianming

2013-08-01

100

Development of an amylolytic Lactobacillus plantarum silage strain expressing the Lactobacillus amylovorus alpha-amylase gene.  

PubMed Central

An amylolytic Lactobacillus plantarum silage strain with the starch-degrading ability displayed by Lactobacillus amylovorus was developed. An active fragment of the gene coding for alpha-amylase production in L. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain L. plantarum Lp80 at the cbh locus. The alpha-amylase gene fragment was also introduced into L. plantarum Lp80 on an autoreplicative plasmid. Both constructions were also performed in the laboratory strain L. plantarum NCIB8826. All four recombinant strains secreted levels of amylase ranging from 23 to 69 U/liter, compared with 47 U/liter for L. amylovorus. Secretion levels were higher in L. plantarum NCIB8826 than in L. plantarum Lp80 derivatives and were higher in recombinant strains containing autoreplicative plasmids than in the corresponding integrants. The L. plantarum Lp80 derivative containing the L. amylovorus alpha-amylase gene fragment integrated into the host chromosome secreted alpha-amylase to a level comparable to that of L. amylovorus and was stable over 50 generations of growth under nonselective conditions. It grew to a higher cell density than either the parent strain or L. amylovorus in MRS medium containing a mixture of starch and glucose as the fermentable carbohydrate source. This recombinant alpha-amylolytic L. plantarum strain would therefore seem to have considerable potential as a silage inoculant for crops such as alfalfa, in which water-soluble carbohydrate levels are frequently low but starch is present as an alternative carbohydrate source. Images

Fitzsimons, A; Hols, P; Jore, J; Leer, R J; O'Connell, M; Delcour, J

1994-01-01

101

Viable versus inactivated lactobacillus strain GG in acute rotavirus diarrhoea  

Microsoft Academic Search

The effect of viable or heat inactivated human Lactobacillus casei strain GG on rotavirus immune responses in patients with rotavirus diarrhoea was assessed. Rotavirus serum IgA enzyme immunoassay antibody responses were higher in infants treated with viable L casei strain GG than in those treated with inactivated L casei strain GG. There was a significant difference at convalescence with rotavirus

M Kaila; E Isolauri; M Saxelin; H Arvilommi; T Vesikari

1995-01-01

102

Adherence of Lactobacillus Species to Human Fetal Intestinal Cells1  

Microsoft Academic Search

Thirty-two lactobacilli were tested for ability to adhere to a human fetal in- testinal epithelial cell line. By an in vitro system, two adherence mechanisms were found. One mechanism, requiring calcium in the adherence reaction, was nonspecific and allowed all lactobacilli tested to adhere. The other system, not requiring calcium, was found in four strains, all human Lactobacillus acidopbilus isolates.

E. G. Kleeman; T. R. Klaenhammer

1982-01-01

103

Biochemical and molecular characterization of Lactobacillus reuteri 121 reuteransucrase  

Microsoft Academic Search

Lactobacillus reuteri strain 121 uses sucrose for synthesis of a unique, soluble glucan (‘reuteran’) with mainly ?-(1?4) glucosidic linkages. The gene (gtfA) encoding this glucansucrase enzyme had previously been characterized. Here, a detailed biochemical and molecular analysis of the GTFA enzyme is presented. This is believed to be the first report describing reuteransucrase enzyme kinetics and the oligosaccharides synthesized with

S. Kralj; G. H. van Geel-Schutten; M. J. E. C. van der Maarel; L. Dijkhuizen

2004-01-01

104

Effect of glucose on glycerol bioconversion by Lactobacillus reuteri  

Microsoft Academic Search

The impact of glucose on glycerol metabolism, especially on 3-hydroxypropionaldehyde (3-HPA) accumulation by resting cells of Lactobacillus reuteri has been investigated. Two systems were used in the study: MRS- (modified MRS - omitting glucose, acetate and Tween 80) and distilled water (H2O). In MRS-, addition of glucose enhanced glycerol metabolism in resting cells of L. reuteri, consequently increasing the accumulation

Q. Lüthi-Peng; F. Dileme; Z. Puhan

2002-01-01

105

Production and stability of 3-hydroxypropionaldehyde in Lactobacillus reuteri  

Microsoft Academic Search

3-Hydroxypropionaldehyde (3-HPA) is considered as a potent antimicrobial substance. Exploration of its application as a food preservative or as a therapeutic auxiliary agent has been documented in the literature. In the present work, factors that may impact on 3-HPA accumulation by Lactobacillus reuteri and on the stability of 3-HPA were investigated. Three media - H2O, milk and MRS broth -

Q. Lüthi-Peng; S. Schärer; Z. Puhan

2002-01-01

106

Kinetic properties of an inulosucrase from Lactobacillus reuteri 121  

Microsoft Academic Search

Inulosucrases catalyze transfer of a fructose moiety from sucrose to a water molecule (hydrolysis) or to an acceptor molecule (transferase), yielding inulin. Bacterial inulin production is rare and a biochemical analysis of inulosucrase enzymes has not been reported. Here we report biochemical characteristics of a purified recombinant inulosucrase enzyme from Lactobacillus reuteri. It displayed Michaelis–Menten type of kinetics with substrate

M. J. E. C. van der Maarel; L. Dijkhuizen

2003-01-01

107

Combination of marker genes for characterizing a Lactobacillus sakei strain  

US Patent & Trademark Office Database

The present invention relates to a new combination of marker genes for characterizing a Lactobacillus sakei strain. In particular, the present invention concerns the use of a pattern of presence or absence of marker genes in the genome of the strain to be characterized for classifying and identifying said strain.

2012-12-11

108

Lactobacillus casei potentiates induction of oral tolerance in experimental arthritis  

Microsoft Academic Search

Probiotics have been shown to exert beneficial effects on modulation of diverse diseases. However, no information is available for the effect of probiotics in the induction of oral tolerance in autoimmune diseases. The main purpose of this study was to elucidate whether Lactobacillus casei (L. casei) affect the induction of oral tolerance in experimental rheumatoid arthritis (RA). Type II collagen

Jae-Seon So; Choong-Gu Lee; Ho-Keun Kwon; Hwa-Jung Yi; Chang-Suk Chae; Jin-A Park; Ki-Chul Hwang; Sin-Hyeog Im

2008-01-01

109

Draft Genome Sequence of Lactobacillus gasseri Strain 2016  

PubMed Central

Different common factors contribute to the antagonistic properties of Lactobacillus gasseri toward various pathogens. However, there is strain-to-strain variation in the probiotic properties of this bacterium. The draft genome sequence of L. gasseri strain 2016 determined in this study will assist in understanding the genetic basis for such variation.

Melnikov, Vyacheslav G.; Kosarev, Igor V.; Khlebnikov, Valentin C.; Sukhikh, Gennady T.; Abramov, Vyacheslav M.

2013-01-01

110

Draft Genome Sequence of Probiotic Strain Lactobacillus rhamnosus R0011  

PubMed Central

Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain.

Barreau, Guillaume; de Carvalho, Vanessa G.

2012-01-01

111

Myoglobin as an Inhibitor of Exopeptidases from Lactobacillus sake  

PubMed Central

The effects of myoglobin on exopeptidases of Lactobacillus sake were determined. Inhibition of the aminopeptidases increased as the myoglobin concentration increased; aminopeptidase 3 was the most affected (90% inhibition). Aminopeptidases 1, 2, and 4 showed similar inhibition levels (around 60%). Myoglobin did not affect tripeptidase activity. Thus, myoglobin could limit amino acid generation in meat systems.

Sanz, Yolanda; Toldra, Fidel

1998-01-01

112

Draft genome sequence of probiotic strain Lactobacillus rhamnosus R0011.  

PubMed

Lactobacillus rhamnosus R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain. PMID:22275100

Tompkins, Thomas A; Barreau, Guillaume; de Carvalho, Vanessa G

2012-02-01

113

Role of transporter proteins in bile tolerance of Lactobacillus acidophilus.  

PubMed

Lactobacillus acidophilus NCFM derivatives containing deletion mutations in the transporter genes LBA0552, LBA1429, LBA1446, and LBA1679 exhibited increased sensitivity to bile. These strains showed unique patterns of sensitivity to a variety of inhibitory compounds, as well as differential accumulations of ciprofloxacin and taurocholate. PMID:19633113

Pfeiler, Erika A; Klaenhammer, Todd R

2009-09-01

114

Antibacterial Effect of the Adhering Human Lactobacillus acidophilus Strain LB  

Microsoft Academic Search

The spent culture supernatant of the human Lactobacillus acidophilus strain LB produces an antibacterial activity against a wide range of gram-negative and gram-positive pathogens. It decreased the in vitro viability of Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Shigella flexneri, Escherichia coli, Kleb- siella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, and Enterobacter spp. In contrast, it did not inhibit lactobacilli and bifidobacteria.

MARIE-HELENE COCONNIER; VANESSA LIEVIN; MARIE-FRANCOISE BERNET-CAMARD; SYLVIE HUDAULT; ALAIN L. SERVIN

1997-01-01

115

Sequence of ornithine decarboxylase from Lactobacillus sp. strain 30a.  

PubMed Central

A gene encoding biodegradative ornithine decarboxylase from Lactobacillus sp. strain 30a was isolated from a genomic DNA library and sequenced. Primer extension analysis revealed two transcription initiation sites. The deduced amino acid sequence is compared with the amino acid sequences of five previously reported bacterial decarboxylases, and conserved pyridoxal phosphate motif residues are identified.

Hackert, M L; Carroll, D W; Davidson, L; Kim, S O; Momany, C; Vaaler, G L; Zhang, L

1994-01-01

116

Draft Genome Sequence of Lactobacillus rossiae DSM 15814T  

PubMed Central

The draft genome sequence of Lactobacillus rossiae DSM 15814T (CS1, ATCC BAA-88) was determined by a whole-genome shotgun approach. Reads were assembled to a 2.9-Mb draft version. RAST genome annotation evidenced 2,723 predicted coding sequences. Many carbohydrate, amino acid, and amino acid derivative subsystem features were found.

Di Cagno, Raffaella; Cattonaro, Federica; Gobbetti, Marco

2012-01-01

117

The PTS transporters of Lactobacillus gasseri ATCC 33323  

Microsoft Academic Search

BACKGROUND: Lactobacilli can utilize a variety of carbohydrates which reflects the nutrient availability in their respective environments. A common lactobacilli in the human gastrointestinal tract, Lactobacillus gasseri, was selected for further study. The currently available annotation of the L. gasseri ATCC 33323 genome describes numerous putative genes involved in carbohydrate utilization, yet the specific functions of many of these genes

Alyssa L Francl; Taksawan Thongaram; Michael J Miller

2010-01-01

118

Antibacterial activity of Lactobacillus sake isolated from meat.  

PubMed Central

A total of 221 strains of Lactobacillus isolated from meat and meat products were screened for antagonistic activities under conditions that eliminated the effects of organic acids and hydrogen peroxide. Nineteen strains of Lactobacillus sake, three strains of Lactobacillus plantarum, and one strain of Lactobacillus curvatus were shown to inhibit the growth of some other lactobacilli in an agar spot test; and cell-free supernatants from 6 of the 19 strains of L. sake exhibited inhibitory activity against indicator organisms. Comparison of the antimicrobial spectra of the supernatants suggested that the inhibitory compounds were not identical. One of the six strains, L. sake Lb 706, was chosen for further study. The compound excreted by L. sake Lb 706 was active against various lactic acid bacteria and Listeria monocytogenes. Its proteinaceous nature, narrow inhibitory spectrum, and bactericidal mode of action indicated that this substance is a bacteriocin, which we designated sakacin A. Curing experiments with two bacteriocin-producing strains of L. sake resulted in mutants that lacked both bacteriocin activity and immunity to the bacteriocin. Plasmid profile analysis of L. sake Lb 706 and two bacteriocin-negative variants of this strain indicated that a plasmid of about 18 megadaltons may be involved in the formation of bacteriocin and immunity to this antibacterial compound. In mixed culture, the bacteriocin-sensitive organisms were killed after the bacteriocin-producing strain reached maximal cell density, whereas there was no decrease in cell number in the presence of the bacteriocin-negative variant. Images

Schillinger, U; Lucke, F K

1989-01-01

119

Lactobacillus rhamnosus polynucleotides, polypeptides and methods for using them  

US Patent & Trademark Office Database

Novel polynucleotides isolated from Lactobacillus rhamnosus, as well as probes and primers, genetic constructs comprising the polynucleotides, biological materials, including plants, microorganisms and multicellular organisms incorporating the polynucleotides, polypeptides expressed by the polynucleotides, and methods for using the polynucleotides and polypeptides are disclosed.

2006-05-30

120

Probiotic-Associated Aspiration Pneumonia Due to Lactobacillus rhamnosus.  

PubMed

Lactobacilli are low-virulence, commensal organisms of the gastrointestinal and genitourinary tracts and are commonly used as "probiotic supplements." Herein, we describe an episode of respiratory syncytial virus (RSV) bronchiolitis with bacterial superinfection secondary to administration of Lactobacillus rhamnosus in an 11-month-old female with trisomy 21. PMID:24899028

Doern, Christopher D; Nguyen, Sean T; Afolabi, Folashade; Burnham, Carey-Ann D

2014-08-01

121

The Requirement for Calcium in Infection with Lactobacillus Phage  

Microsoft Academic Search

SUMMARY Calcium ions were specifically required for a stage in the formation of phage- infected cells in the growth cycle of PL-I phage active against Lactobacillus casei ATCC 27092. Both the adsorption of phage on to the host cells and the intracellular multiplication of phage were independent of the presence or absence of calcium ions. The optimum pH for successful

K. Watanabe; S. Takesue

1972-01-01

122

Genome Sequence of Lactobacillus plantarum Strain UCMA 3037.  

PubMed

Nucleic acid of the strain Lactobacillus plantarum UCMA 3037, isolated from raw milk camembert cheese in our laboratory, was sequenced. We present its draft genome sequence with the aim of studying its functional properties and relationship to the cheese ecosystem. PMID:23704179

Naz, Saima; Tareb, Raouf; Bernardeau, Marion; Vaisse, Melissa; Lucchetti-Miganeh, Celine; Rechenmann, Mathias; Vernoux, Jean-Paul

2013-01-01

123

Antimicrobial activity of Lactobacillus against microbial flora of cervicovaginal infections  

PubMed Central

Objective To assess the probiotic nature of Lactobacillus in preventing cervical pathogens by studying the effectiveness of antimicrobial activity against vaginal pathogens. Methods Lactobacilli were isolated from healthy vaginal swabs on selective media and different pathogenic bacteria were isolated by using different selective media. The Lactobacillus strains were tested for the production of hydrogen peroxide and antimicrobial compounds along with probiotic properties. Results Of the 10 isolated Lactobacillus strains, strain 1, 3 and 6 are high hydrogen peroxide producers and the rest were low producers. Results of pH and amines tests indicated that pH increased with fishy odour in the vaginal fluids of cervicovaginal infection patients when compared with vaginal fluids of healthy persons. The isolates were found to be facultative anaerobic, Gram-positive, non-spore-forming, non-capsule forming and catalase-negative bacilli. The results of antimicrobial activity of compounds indicated that 280 and 140 µg/mL was the minimum concentration to inhibit the growth of both pathogens and test organisms respectively. Conclusions The results demonstrated that Lactobacillus producing antimicrobial compounds inhibits the growth of cervical pathogens, revealing that the hypothesis of preventing vaginal infection by administering probiotic organisms has a great appeal to patients, which colonize the vagina to help, restore and maintain healthy vagina.

Dasari, Subramanyam; Shouri, Raju Naidu Devanaboyaina; Wudayagiri, Rajendra; Valluru, Lokanatha

2014-01-01

124

Metabolism of biodiesel-derived glycerol in probiotic Lactobacillus strains.  

PubMed

Three probiotic Lactobacillus strains, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus delbrueckii, were tested for their ability to assimilate and metabolize glycerol. Biodiesel-derived glycerol was used as the main carbon and energy source in batch microaerobic growth. Here, we show that the tested strains were able to assimilate glycerol, consuming between 38 and 48 % in approximately 24 h. L. acidophilus and L. delbrueckii showed a similar growth, higher than L. plantarum. The highest biomass reached was 2.11 g?L?¹ for L. acidophilus, with a cell mass yield (Y (X/S)) of 0.37 g?g?¹. L. delbrueckii and L. plantarum reached a biomass of 2.06 and 1.36 g?L?¹. All strains catabolize glycerol mainly through glycerol kinase (EC 2.7.1.30). For these lactobacillus species, kinetic parameters for glycerol kinase showed Michaelis-Menten constant (K(m)) ranging from 1.2 to 3.8 mM. The specific activities for glycerol kinase in these strains were in the range of 0.18 to 0.58 U?mg?protein?¹, with L. acidophilus ATCC 4356 showing the maximum specific activity after 24 h of cultivation. Glycerol dehydrogenase activity was also detected in all strains studied but only for the reduction of glyceraldehyde with NADPH (K(m) for DL-glyceraldehyde ranging from 12.8 to 32.3 mM). This enzyme shows a very low oxidative activity with glycerol and NADP+ and, most likely, under physiological conditions, the oxidative reaction does not occur, supporting the assumption that the main metabolic flux concerning glycerol metabolism is through the glycerol kinase pathway. PMID:23229571

Rivaldi, Juan Daniel; Sousa Silva, Marta; Sousa Silva, Marta Luís C; Duarte, Luis C; Ferreira, António E N; Cordeiro, Carlos; de Almeida Felipe, Maria das Graças; de Ponces Freire, Ana; de Mancilha, Ismael Maciel

2013-02-01

125

First Functional and Mutational Analysis of Group 3 N-Acetylneuraminate Lyases from Lactobacillus antri and Lactobacillus sakei 23K  

PubMed Central

N-acetyl neuraminate lyases (NALs) catalyze the reversible aldol cleavage of N-acetyl neuraminic acid (Neu5Ac) to pyruvate and N-acetyl-D-mannosamine (ManNAc). Previous phylogenetic studies divided NALs into four different groups. Groups 1 and 2 have been well characterized at both kinetic and molecular levels, but no NAL from group 3 has been studied to date. In this work, a functional characterization of two group 3 members was performed using the recombinant NALs from Lactobacillus antri and Lactobacillus sakei 23K, revealing an optimal pH of between 6.0 and 7.0, low stability at basic pHs (>8.0), low optimal temperatures and, especially, low catalytic efficiency compared with their counterparts in group 1 and 2. The mutational analysis carried out showed that a plausible molecular reason for the low activity shown by Lactobacillus antri and Lactobacillus sakei 23k NALs compared with group 1 and 2 NALs could be the relatively small sugar-binding pocket they contain. A functional divergence analysis concluding that group 3 is more closely related to group 2 than to group 1.

Garcia-Garcia, Maria Inmaculada; Gil-Ortiz, Fernando; Garcia-Carmona, Francisco; Sanchez-Ferrer, Alvaro

2014-01-01

126

Genome Sequence of Lactobacillus saerimneri 30a (Formerly Lactobacillus sp. Strain 30a), a Reference Lactic Acid Bacterium Strain Producing Biogenic Amines  

PubMed Central

Lactobacillus sp. strain 30a (Lactobacillus saerimneri) produces the biogenic amines histamine, putrescine, and cadaverine by decarboxylating their amino acid precursors. We report its draft genome sequence (1,634,278 bases, 42.6% G+C content) and the principal findings from its annotation, which might shed light onto the enzymatic machineries that are involved in its production of biogenic amines.

Romano, Andrea; Trip, Hein; Campbell-Sills, Hugo; Bouchez, Olivier; Sherman, David; Lolkema, Juke S.

2013-01-01

127

Heat Shock Response in Lactobacillus plantarum  

PubMed Central

Heat stress resistance and response were studied in strains of Lactobacillus plantarum. Stationary-phase cells of L. plantarum DPC2739 had decimal reduction times (D values) (D value was the time that it took to reduce the number of cells by 1 log cycle) in sterile milk of 32.9, 14.7, and 7.14 s at 60, 72, and 75°C, respectively. When mid-exponential-phase cells were used, the D values decreased. The temperature increases which caused a 10-fold reduction in the D value ranged from 9 to 20°C, depending on the strain. Part of the cell population treated at 72°C for 90 s recovered viability during incubation at 7°C in sterile milk for 20 days. When mid-exponential- or stationary-phase cells of L. plantarum DPC2739 were adapted to 42°C for 1 h, the heat resistance at 72°C for 90 s increased ca. 3 and 2 log cycles, respectively. Heat-adapted cells also showed increased growth at pH 5 and in the presence of 6% NaCl. Two-dimensional gel electrophoresis of proteins expressed by control and heat-adapted cells revealed changes in the levels of expression of 31 and 18 proteins in mid-exponential- and stationary-phase cells, respectively. Twelve proteins were commonly induced. Nine proteins induced in the heat-adapted mid-exponential- and/or stationary-phase cells of L. plantarum DPC2739 were subjected to N-terminal sequencing. These proteins were identified as DnaK, GroEL, trigger factor, ribosomal proteins L1, L11, L31, and S6, DNA-binding protein II HlbA, and CspC. All of these proteins have been found to play a role in the mechanisms of stress adaptation in other bacteria. Antibodies against GroES detected a protein which was induced moderately, while antibodies against DnaJ and GrpE reacted with proteins whose level of expression did not vary after heat adaptation. This study showed that the heat resistance of L. plantarum is a complex process involving proteins with various roles in cell physiology, including chaperone activity, ribosome stability, stringent response mediation, temperature sensing, and control of ribosomal function. The physiological mechanisms of response to pasteurization in L. plantarum are fundamental for survival in cheese during manufacture.

De Angelis, Maria; Di Cagno, Raffaella; Huet, Claude; Crecchio, Carmine; Fox, Patrick F.; Gobbetti, Marco

2004-01-01

128

Lactobacillus role during conditioning of refrigerated and vacuum-packaged Argentinean meat.  

PubMed

The role of Lactobacillus strains with bioprotective and technological potential on raw beef during 15days of storage under vacuum at 7°C was investigated. The assayed strains were able to grow on the meat, Lactobacillus curvatus CRL705 and Lactobacillus sakei 23K showing the highest competitiveness. A net increase of amino acids was determined in inoculated samples when compared to the control, this being maximal for Lactobacillus plantarum CRL681. Although an important endogenous activity of meat sarcoplasmic proteins was observed, the disappearance of protein bands and the generation of a new one were detected as a consequence of Lactobacillus growth. A synergistic effect of Lactobacillus in combination with the muscle proteolytic enzyme complex can be suggested. From the studied strains, the bacteriocin producer L. curvatus CRL705 may be considered as a good candidate to contribute to meat ageing by means of small peptides and free amino acids generation while improving shelf life. PMID:22062923

Fadda, S; Chambon, C; Champomier-Vergès, M C; Talon, R; Vignolo, G

2008-07-01

129

Lactobacillus and Bifidobacterium Diversity in Horse Feces, Revealed by PCR-DGGE  

Microsoft Academic Search

Lactobacillus equi, Lactobacillus hayakitensis, Lactobacillus johnsonii, and Weissella confusa\\/cibaria were the dominant species in 12 South African horses. The Bifidobacterium-group was detected in the feces of only one of the 12 horses. Sequencing of the nested-PCR amplicon identified the Bifidobacterium-group as Parascardovia denticolens. Cell numbers of L. equi, L. hayakitensis, and W. confusa\\/cibaria were consistent in all samples. P. denticolens,

Akihito Endo; Y. Futagawa-Endo; L. M. T. Dicks

2009-01-01

130

Genetic and Biochemical Characterization of theLactobacillus delbrueckiisubsp.lactisBacteriophage LL-H Lysin  

Microsoft Academic Search

LL-H, a virulent phage ofLactobacillus delbrueckiisubsp.lactis, produces a peptidoglycan-degrading enzyme, Mur, that is effective on L. delbrueckii, Lactobacillus acidophilus, Lactobacillus helveticus, and Pediococcus damnosuscell walls. In this study, the LL-H genemurwas cloned intoEscherichia coli, its nucleotide sequence was determined, and the enzyme produced in E. coli was purified and biochemically characterized. Mur was purified 112-fold by means of ammonium sulfate

ANTTI VASALA; MERJA VALKKILA; JAVIER CALDENTEY; ANDTAPANI ALATOSSAVA

1995-01-01

131

Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract  

Microsoft Academic Search

One strain of Lactobacillus salivarius, two strains of Lactobacillus reuteri and Lactobacillus amylovorus, and two strains of Bifidobacterium thermacidophilum with antagonistic effect against Clostridium perfringens were isolated from porcine gastrointestinal tract. Isolates were assayed for their ability to survive in synthetic gastric\\u000a juice at pH 2.5 and were examined for their ability to grow on agar plate containing porcine bile extract.

Pyoung Il Kim; Min Young Jung; Young-Hyo Chang; Saehun Kim; Seong-Jae Kim; Yong-Ha Park

2007-01-01

132

Colonization and Fecal Enzyme Activities after Oral Lactobacillus GG Administration in Elderly Nursing Home Residents  

Microsoft Academic Search

This study was undertaken to investigate the effect of 2-week oral administration of a Lactobacillus-GG-fermentedwhey drink on fecal enzymes in elderly nursing home residents complaining of difficulties in defecation. The study was divided into three 2-week periods: baseline (2 dl placebo drink\\/day); Lactobacillus GG treatment (2 dl of a Lactobacillus-GG-fermented whey drink containing 108 cfu lactobacilli\\/ml); follow-up (2 dl placebo

W. H. Ling; O. Hänninen; H. Mykkänen; M. Heikura; S. Salminen; A. Von Wright

1992-01-01

133

Should Lactobacillus sporogenes and Bacillus coagulans have a future?  

PubMed

Probiotics are gaining increasing scientific and commercial interest as functional foods. Their success has led to the development and marketing of a broad range of products based on probiotics. In this context, resolution of the taxonomy of microbial species remains a key point, since different species belonging to the same genus may have different beneficial properties. Lactobacillus sporogenes, which should be correctly classified as Bacillus coagulans, represents the archetypal misidentified probiotic and its inclusion among probiotics has often been a matter of debate. Since this bacterium shows characteristics of both genera Lactobacillus and Bacillus, its taxonomic position between the families lactobacillaceae and bacillaceae has often been discussed.This review summarizes the salient probiotic features of L. sporogenes /B. coagulans by examining currently available information. Although the use of L. sporogenes spores as a probiotic has increased in recent years, clinical evidence of its benefits are limited to only a few studies involving small patient populations. PMID:19622453

Drago, L; De Vecchi, E

2009-08-01

134

Biowaste: a Lactobacillus habitat and lactic acid fermentation substrate.  

PubMed

Composite organic waste was assessed for its physical, chemical and microbial suitability to serve as a substrate for the fermentative production of lactic acid. The biowaste studied was highly acidic (pH 4.3) and had high organic carbon content (45%). A clone library identified 90% of the bacterial community were lactic acid bacteria, mainly represented by Lactobacilli (70%). Cultivation using semiselective media identified Lactobacillus plantarum, Lactobacillus brevis and their closest relatives as the dominating taxa. PCR-DGGE using general bacterial and lactic acid bacterial specific primers resulted in little heterogeneity of microbial community. These data indicate that biowaste is a preferred habitat of lactic acid bacteria, suggesting that the unsterilized biowaste and its natural flora could be used in a fermentation process for lactic acid production. Such kind of biowaste application could be an alternative for current substrates and provide a modern, efficient and environmental friendly waste treatment technology. PMID:23816359

Probst, Maraike; Fritschi, Annika; Wagner, Andreas; Insam, Heribert

2013-09-01

135

Comparison of the Phospholipid Composition of Bifidobacterium and Lactobacillus Strains  

PubMed Central

Phospholipid composition of 10 Bifidobacterium strains of human intestinal origin and of 9 Lactobacillus strains was determined by quantitative two-dimensional thin-layer chromatography. Phospholipids of three Bifidobacterium strains from honey bees and of two strains from bovine rumen liquor were qualitatively investigated. Diphosphatidylglycerol and phosphatidylglycerol were present in strains of both genera. All Bifidobacterium strains contained as specific phospholipids a new polyglycerolphospholipid, compound 15, and its lyso derivatives, earlier detected in B. bifidum var. pennsylvanicus. Also, lyso compounds of diphosphatidylglycerol and alanyl phosphatidylglycerol were only present in this genus in variable amounts. Lysyl phosphatidylglycerol was the only ninhydrin-positive phospholipid in seven Lactobacillus strains. In L. delbrückii and L. helveticus it was absent and partially replaced by an unidentified ninhydrin-negative phospholipid. The differences in phospholipid composition between bifidobacteria and lactobacilli may be another argument to differentiate these two genera. Images

Exterkate, F. A.; Otten, B. J.; Wassenberg, H. W.; Veerkamp, J. H.

1971-01-01

136

Complete Genome Sequence of Probiotic Strain Lactobacillus acidophilus La-14.  

PubMed

We present the 1,991,830-bp complete genome sequence of Lactobacillus acidophilus strain La-14 (SD-5212). Comparative genomic analysis revealed 99.98% similarity overall to the L. acidophilus NCFM genome. Globally, 111 single nucleotide polymorphisms (SNPs) (95 SNPs, 16 indels) were observed throughout the genome. Also, a 416-bp deletion in the LA14_1146 sugar ABC transporter was identified. PMID:23788546

Stahl, Buffy; Barrangou, Rodolphe

2013-01-01

137

Complete Genome Sequence of Probiotic Strain Lactobacillus acidophilus La-14  

PubMed Central

We present the 1,991,830-bp complete genome sequence of Lactobacillus acidophilus strain La-14 (SD-5212). Comparative genomic analysis revealed 99.98% similarity overall to the L. acidophilus NCFM genome. Globally, 111 single nucleotide polymorphisms (SNPs) (95 SNPs, 16 indels) were observed throughout the genome. Also, a 416-bp deletion in the LA14_1146 sugar ABC transporter was identified.

Stahl, Buffy

2013-01-01

138

Formation of tyramine by Lactobacillus curvatus LTH 972  

Microsoft Academic Search

Zusammenfassung Der Einfluß von Lebensmittel kennzeichnenden ökologischen Faktoren auf die Bildung von Tyramin durchLactobacillus curvatus LTH 972 wurde in Flüssigkultur mit Tyrosinzusatz untersucht. Höchste Konzentrationen an Tyramin (bis zu 201 mg\\/l) wurden bei 30 °C, pH 5,2 und aw 0,97 gebildet. Bei niedrigeren Temperaturen (15 °C) sowie höheren pH- und aw-Werten wurde eine verlangsamte, aber dennoch deutliche Tyraminbildung beobachtet. Glucose,

Bernhard W. Straub; Petra S. Tichaczek; Martin Kicherer; Walter P. Hammes

1994-01-01

139

Heterogeneity of Putative Surface Layer Proteins in Lactobacillus helveticus  

PubMed Central

The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridization. This study demonstrates S-layer gene variability in the species L. helveticus.

Gatti, Monica; Rossetti, Lia; Fornasari, Maria Emanuela; Lazzi, Camilla; Giraffa, Giorgio; Neviani, Erasmo

2005-01-01

140

Purification and cloning of a protein secreted from lactobacillus acidophilus  

Microsoft Academic Search

Among the proteins secreted from Lactobacillus acidophilus KCTC 3151, a 36 kDa and 24 kDa protein, whose amounts were relatively abundant, were purified and their N?terminal amino acid sequences determined. The N?terminal amino acid sequence of 36 kDa protein exhibited high homology with thymidine phosphorylase and glyceraldehyde?3?phosphate dehydrogenase. The N?terminal amino acid sequence of the 24 kDa protein did not

Seoyoung Han; Yong Sun Lee; Deog Su Hwang

1998-01-01

141

Comparative Genomics of the Late Gene Cluster from Lactobacillus Phages  

Microsoft Academic Search

Three prophage sequences were identified in the Lactobacillus johnsoni strain NCC533. Prophage Lj965 predicted a gene map very similar to those of pac-site Streptococcus thermophilus phages over its DNA packaging and head and tail morphogenesis modules. Sequence similarity linked the putative DNA packaging and head morphogenesis genes at the protein level. Prophage Lj965\\/S. thermophilus phage Sfi11\\/Lactococcus lactis phage TP901-1 on

Frank Desiere; R. David Pridmore; Harald Brüssow

2000-01-01

142

Lactobacillus kimchiensis sp. nov., isolated from a fermented food.  

PubMed

A novel bacterium was isolated from a traditional fermented food, kimchi. The morphology, physiology, biochemical properties and 16S rRNA gene sequence of strain L133(T) were studied. Strain L133(T) was Gram-reaction-positive, catalase-negative and homofermentative, with rod-shaped cells that formed cream colonies. Cells grew in the presence of 0-5?% (w/v) NaCl (optimum, 1-2?%), at pH 5.0-9.0 (optimum, pH 7.0-8.0) and at 15-37 °C (optimum, 25 °C). Comparative 16S rRNA gene and pheS sequence analysis of strain L133(T) indicated that the strain belonged to the genus Lactobacillus. The major fatty acids were identified as C18?:?1?9c, C16?:?0 and C18?:?0, and the cell wall contained peptidoglycan of the L-Lys-D-Asp type. DNA-DNA relatedness values between strain L133(T) and related species were below 11±0.4?%. The DNA G+C content of strain L133(T) was 35.7 mol%. Analysis of 16S rRNA gene sequences, as well as physiological and biochemical tests, identified genotypic and phenotypic differences between strain L133(T) and other species of the genus Lactobacillus. Based on these analyses, strain L133(T) is proposed to be a novel species of the genus Lactobacillus, named Lactobacillus kimchiensis. The type strain is L133(T) (?=?KACC 15533(T)?=?JCM 17702(T)?=?DSM 24716(T)). PMID:22798657

Kim, Jandi; Kim, Joon Yong; Kim, Min-Soo; Roh, Seong Woon; Bae, Jin-Woo

2013-04-01

143

Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains.  

PubMed

The objective of this study was to evaluate the functional properties of lactic acid bacteria (LAB) isolated from Tibetan kefir grains. Three Lactobacillus isolates identified as Lactobacillus acidophilus LA15, Lactobacillus plantarum B23 and Lactobacillus kefiri D17 that showed resistance to acid and bile salts were selected for further evaluation of their probiotic properties. The 3 selected strains expressed high in vitro adherence to Caco-2 cells. They were sensitive to gentamicin, erythromycin and chloramphenicol and resistant to vancomycin with MIC values of 26 µg/ml. All 3 strains showed potential bile salt hydrolase (BSH) activity, cholesterol assimilation and cholesterol co-precipitation ability. Additionally, the potential effect of these strains on plasma cholesterol levels was evaluated in Sprague-Dawley (SD) rats. Rats in 4 treatment groups were fed the following experimental diets for 4 weeks: a high-cholesterol diet, a high-cholesterol diet plus LA15, a high-cholesterol diet plus B23 or a high-cholesterol diet plus D17. The total cholesterol, triglyceride and low-density lipoprotein cholesterol levels in the serum were significantly (P<0.05) decreased in the LAB-treated rats compared with rats fed a high-cholesterol diet without LAB supplementation. The high-density lipoprotein cholesterol levels in groups B23 and D17 were significantly (P<0.05) higher than those in the control and LA15 groups. Additionally, both fecal cholesterol and bile acid levels were significantly (P<0.05) increased after LAB administration. Fecal lactobacilli counts were significantly (P<0.05) higher in the LAB treatment groups than in the control groups. Furthermore, the 3 strains were detected in the rat small intestine, colon and feces during the feeding trial. The bacteria levels remained high even after the LAB administration had been stopped for 2 weeks. These results suggest that these strains may be used in the future as probiotic starter cultures for manufacturing novel fermented foods. PMID:23894554

Zheng, Yongchen; Lu, Yingli; Wang, Jinfeng; Yang, Longfei; Pan, Chenyu; Huang, Ying

2013-01-01

144

From the Cover: Multireplicon genome architecture of Lactobacillus salivarius  

Microsoft Academic Search

Lactobacillus salivarius subsp. salivarius strain UCC118 is a bacteriocin-producing strain with probiotic characteristics. The 2.13-Mb genome was shown by sequencing to comprise a 1.83 Mb chromosome, a 242-kb megaplasmid (pMP118), and two smaller plasmids. Megaplasmids previously have not been characterized in lactic acid bacteria or intestinal lactobacilli. Annotation of the genome sequence indicated an intermediate level of auxotrophy compared with

Marcus J. Claesson; Yin Li; Sinead Leahy; Carlos Canchaya; Jan Peter van Pijkeren; Ana M. Cerdeño-Tárraga; Julian Parkhill; Sarah Flynn; Gerald C. O'Sullivan; J. Kevin Collins; Des Higgins; Fergus Shanahan; Gerald F. Fitzgerald; Douwe van Sinderen; Paul W. O'Toole

2006-01-01

145

Purification and characterization of a dipeptidase from Lactobacillus sake.  

PubMed Central

A dipeptidase was purified from cell extracts of Lactobacillus sake. This compound was a monomer having a molecular weight of 50,000 and a pI of 4.7 and exhibited broad specificity against all dipeptides except those with proline or glycine at the N terminus. The enzyme was inhibited by EDTA or 1,10-phenanthroline but could be reactivated with CoCl2 and MnCl2.

Montel, M C; Seronie, M P; Talon, R; Hebraud, M

1995-01-01

146

Physiological responses to folate overproduction in Lactobacillus plantarum WCFS1  

Microsoft Academic Search

BACKGROUND: Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in Lactobacillus plantarum WCFS1. We focused specifically on the mechanism that reduces growth rates in folate-overproducing cells. RESULTS: Metabolite formation and gene expression were determined in a folate-overproducing- and wild-type strain. Differential metabolomics analysis of intracellular metabolite pools indicated that the

Arno Wegkamp; Astrid E Mars; Magda Faijes; Douwe Molenaar; Ric CH de Vos; Sebastian MJ Klaus; Andrew D Hanson; Willem M de Vos; Eddy J Smid

2010-01-01

147

Inducible Promoter-Repressor System from the Lactobacillus casei  

Microsoft Academic Search

With the aim to extend the presently available inducible gene expression systems for lactobacilli, we have isolated a thermoinducible promoter-repressor cassette from the temperate Lactobacillus casei phage FSW-TI in Escherichia coli. The FSW-TI promoter fragment was abutted to the plasmid-borne promoterless -glu- curonidase (gusA) reporter gene and shown to direct its transcription in L. casei. In addition, the functionality of

Phage FSW; Bernhard Binishofer; Isabella Moll; Bernhard Henrich; Udo Blasi

2002-01-01

148

Development of an immunochemical method to detect Lactobacillus kefir  

Microsoft Academic Search

Kefir is a fermented milk elaborated with kefir grains. It constitutes an ecological system composed by a protein-polysaccharide matrix on which a complex microbiota is naturally immobilized. The objective of this work was to develop an antibody-based method in order to achieve a simple and rapid way to quantify Lactobacillus kefir in a complex environment. Rabbit antisera against isolated S-layer

Graciela L. Garrote; Maria A. Serradell; Analia G. Abraham; Maria C. Añon; Carlos A. Fossati; Graciela L. De Antoni

2005-01-01

149

Antitumor Activity of Lactobacillus plantarum Cytoplasm on Teratocarcinoma Bearing Mice  

Microsoft Academic Search

Potential antitumor activity of Lactobacillus plantarum cytoplasm was examined using F9 teratocarcinoma-bearing BALB\\/c mice. The cytoplasmic fraction of L. plantarum was separated by sonication followed by ultracentrifugation. The fraction at a dose of 100 or 200 mg\\/kg\\/day was orally administered for 7 consecutive days before or after tumor inoculation to 16 mice. As a control, heat-killed whole cell was used

KYOUNG HEON KIM; EUNG-RYOOL KIM; HOO-KIL JUNG; HO-NAM JUHN; HYONG JOO LEE

2002-01-01

150

Survival of Lactobacillus plantarum in model solutions and fruit juices  

Microsoft Academic Search

The aim of the work was to study the survival of Lactobacillus plantarum NCIMB 8826 in model solutions and develop a mathematical model describing its dependence on pH, citric acid and ascorbic acid. A Central Composite Design (CCD) was developed studying each of the three factors at five levels within the following ranges, i.e., pH (3.0–4.2), citric acid (6–40g\\/L), and

Sawaminee Nualkaekul; Dimitris Charalampopoulos

2011-01-01

151

Prophylaxis against ampicillin-associated diarrhea with a lactobacillus preparation.  

PubMed

The efficacy of a lactobacillus preparation (Lactinex) in preventing ampicillin-associated diarrhea in adult inpatients was studied. In a double-blind procedure, 98 patients were assigned randomly to receive one packet of Lactinex (Lactobacillus acidophilus and L bulgaricus) or placebo four times daily for the first five days of ampicillin therapy. Cases of diarrhea were reviewed independently and classified according to etiology. Complete data were obtained for 79 patients--43 received placebo and 36 received Lactinex. Diarrhea occurred in nine (21%) patients receiving placebo and in three (8.3%) patients receiving Lactinex; the difference was not statistically significant (p = 0.21, Fisher's Exact Test). When the patients with diarrhea unrelated to ampicillin were excluded (50%), the incidence of ampicillin-induced diarrhea in the placebo group (14%) was significantly greater (p = 0.03, Fisher's Exact Test) than in the Lactinex group (0%). Prophylactic administration of lactobacillus preparations may be effective in preventing ampicillin-induced diarrhea; however, prophylactic administration appears to be justified, on a cost/benefit basis, only in patients with a history of antibiotic-induced diarrhea. PMID:111546

Gotz, V; Romankiewicz, J A; Moss, J; Murray, H W

1979-06-01

152

Lactobacillus with probiotic potential from homemade cheese in Azerbijan  

PubMed Central

Introduction: Lactobacillus is believed to be beneficial in human health, thus the search for isolation and identification of friendly human bacteria from traditional fermented foods is important in medicine. One of the dairy products, traditional cheese as a highly-consumed dairy product could be a valuable source of these friendly edible germs. Methods: In this research, home-made cheese from Lankaran, Jalil Abad and Salian regions in Azerbaijan was characterized for the presence of Lactobacilli with probiotic potential. The bacterial suspension was enriched and screened for acid and bile resistances. Then, the isolates were subjected to antibiotic resistance and antibacterial effects against convenient pathogenic bacteria. The isolates were identified by 16s rDNA sequencing. Results: The results clearly revealed two probiotics with higher homology to Lactobacillus planetarum and Lactobacillus fermentum. Conclusion: No antibiotic resistance was detected in any of the potentially probiotic lactobacilli isolates in these regions, where people continue to follow a life-style that is largely traditional, with traditional medications.

Mojarad Khanghah, Saeed; Ganbarov, Khudaverdi

2014-01-01

153

Characterisation of the vaginal Lactobacillus microbiota associated with preterm delivery.  

PubMed

The presence of an abnormal vaginal microflora in early pregnancy is a risk factor for preterm delivery. There is no investigation on vaginal flora dominated by lactic acid bacteria and possible association with preterm delivery. We assessed the dominant vaginal Lactobacillus species in healthy pregnant women in early pregnancy in relation to pregnancy outcome. We observed 111 low risk pregnant women with a normal vaginal microflora 11 + 0 to 14 + 0 weeks of pregnancy without subjective complaints. Vaginal smears were taken for the identification of lactobacilli using denaturing gradient gel electrophoresis (DGGE). Pregnancy outcome was recorded as term or preterm delivery (limit 36 + 6 weeks of gestation). The diversity of Lactobacillus species in term vs. preterm was the main outcome measure. L. iners alone was detected in 11 from 13 (85%) women who delivered preterm. By contrast, L. iners alone was detected in only 16 from 98 (16%) women who delivered at term (p < 0.001). Fifty six percent women that delivered at term and 8% women that delivered preterm had two or more vaginal Lactobacillus spp. at the same time. This study suggests that dominating L. iners alone detected in vaginal smears of healthy women in early pregnancy might be associated with preterm delivery. PMID:24875844

Petricevic, Ljubomir; Domig, Konrad J; Nierscher, Franz Josef; Sandhofer, Michael J; Fidesser, Maria; Krondorfer, Iris; Husslein, Peter; Kneifel, Wolfgang; Kiss, Herbert

2014-01-01

154

Characterisation of the vaginal Lactobacillus microbiota associated with preterm delivery  

PubMed Central

The presence of an abnormal vaginal microflora in early pregnancy is a risk factor for preterm delivery. There is no investigation on vaginal flora dominated by lactic acid bacteria and possible association with preterm delivery. We assessed the dominant vaginal Lactobacillus species in healthy pregnant women in early pregnancy in relation to pregnancy outcome. We observed 111 low risk pregnant women with a normal vaginal microflora 11 + 0 to 14 + 0 weeks of pregnancy without subjective complaints. Vaginal smears were taken for the identification of lactobacilli using denaturing gradient gel electrophoresis (DGGE). Pregnancy outcome was recorded as term or preterm delivery (limit 36 + 6 weeks of gestation). The diversity of Lactobacillus species in term vs. preterm was the main outcome measure. L. iners alone was detected in 11 from 13 (85%) women who delivered preterm. By contrast, L. iners alone was detected in only 16 from 98 (16%) women who delivered at term (p < 0.001). Fifty six percent women that delivered at term and 8% women that delivered preterm had two or more vaginal Lactobacillus spp. at the same time. This study suggests that dominating L. iners alone detected in vaginal smears of healthy women in early pregnancy might be associated with preterm delivery.

Petricevic, Ljubomir; Domig, Konrad J.; Nierscher, Franz Josef; Sandhofer, Michael J.; Fidesser, Maria; Krondorfer, Iris; Husslein, Peter; Kneifel, Wolfgang; Kiss, Herbert

2014-01-01

155

Lactobacillus with probiotic potential from homemade cheese in Azerbijan.  

PubMed

Introduction: Lactobacillus is believed to be beneficial in human health, thus the search for isolation and identification of friendly human bacteria from traditional fermented foods is important in medicine. One of the dairy products, traditional cheese as a highly-consumed dairy product could be a valuable source of these friendly edible germs. Methods: In this research, home-made cheese from Lankaran, Jalil Abad and Salian regions in Azerbaijan was characterized for the presence of Lactobacilli with probiotic potential. The bacterial suspension was enriched and screened for acid and bile resistances. Then, the isolates were subjected to antibiotic resistance and antibacterial effects against convenient pathogenic bacteria. The isolates were identified by 16s rDNA sequencing. Results: The results clearly revealed two probiotics with higher homology to Lactobacillus planetarum and Lactobacillus fermentum. Conclusion: No antibiotic resistance was detected in any of the potentially probiotic lactobacilli isolates in these regions, where people continue to follow a life-style that is largely traditional, with traditional medications. PMID:24790899

Mojarad Khanghah, Saeed; Ganbarov, Khudaverdi

2014-01-01

156

Comparative Sequence Analyses of the Genes Coding for 16s rRNA of Lactobacillus casei-Related Taxa  

Microsoft Academic Search

The primary structures of the 16s rRNA genes of the type strains of Lactobacillus casei and related taxa were determined by PCR DNA-sequencing methods. The sequences of Lactobacillus casei, Lactobacillus zeae, Lacto- bacillus paracasei, and Lactobacillus rhumnosus were different. The K,,, values ranged from 0.0040 to 0.0126. On the basis of the K,,, values and the levels of DNA-DNA relatedness

KATSUMI MORI; KOJI YAMAZAKI; TOMOHARU ISHIYAMA; KAYO KOBAYASH; HARUO SHINAN

1997-01-01

157

The effect of probiotics (Lactobacillus rhamnosus HN001, Lactobacillus paracasei LPC-37, and Lactobacillus acidophilus NCFM) on the availability of minerals from Dutch-type cheese.  

PubMed

The use of probiotic cultures in the production of Dutch-type cheeses did not lead to significant changes in their chemical composition but it lowered their acidity. The availability of calcium and magnesium analyzed by in vitro enzymatic hydrolysis was 19 and 35%, respectively; the availability of phosphorus was significantly higher, at >90%. The use of probiotic cultures significantly increased the availability of calcium (~2.5%), phosphorus (~6%), and magnesium (~18%). The in vitro method supports accurate determination of the effect of the Lactobacillus spp. cultures on the availability of mineral compounds ingested with Dutch-type cheese. PMID:24913654

Aljewicz, Marek; Siemianowska, Ewa; Cichosz, Gra?yna; To?ska, El?bieta

2014-08-01

158

Systemic augmentation of the immune response in mice by feeding fermented milks with Lactobacillus casei and Lactobacillus acidophilus.  

PubMed Central

This study investigates the effect of feeding fermented milks with Lactobacillus casei, Lactobacillus acidophilus and a mixture of both micro-organisms on the specific and non-specific host defence mechanisms in Swiss mice. Animals fed with fermented milk for 8 days (100 micrograms/day) showed an increase in both phagocytic and lymphocytic activity. This activation of the immune system began on the 3rd day, reached a maximum on the 5th, and decreased slightly on the 8th day of feeding. In the 8-day treated mice, boosted with a single dose (100 micrograms) on the 11th day, the immune response increased further. The feeding with fermented milk produced neither hepatomegaly nor splenomegaly. These results suggest that L. casei and L. acidophilus, associated with intestinal mucosae, can influence the level of activation of the immune system. The possible clinical application of fermented milks as immunopotentiators is also discussed.

Perdigon, G; de Macias, M E; Alvarez, S; Oliver, G; de Ruiz Holgado, A P

1988-01-01

159

Puri¢cation of a novel fructosyltransferase from Lactobacillus reuteri strain 121 and characterization of the levan produced  

Microsoft Academic Search

Fructosyltransferase (FTF) enzymes have been characterized from various Gram-positive bacteria, but not from Lactobacillus sp. In a screening of 182 lactobacilli for polysaccharide production only one strain, Lactobacillus reuteri strain 121, was found to produce a fructan being a levan. Here we report the first-time identification and biochemical characterization of a Lactobacillus FTF enzyme. When incubated with sucrose the enzyme

Sacha A. F. T. van Hijum; Kees Bonting; Marc J. E. C. van der Maarel; Lubbert Dijkhuizen

2001-01-01

160

Fecal recovery following oral administration of Lactobacillus Strain GG (ATCC 53103) in gelatine capsules to healthy volunteers  

Microsoft Academic Search

Recovery of the suggested probiotic strain Lactobacillus GG in feces was studied after oral administration. Lactobacillus GG was given to 20 healthy human volunteers for 7 days in gelatine capsules with daily doses of 1.6 × 108 cfu and 1.2 × 1010 cfu. All the volunteers in the higher dose group had detectable numbers of Lactobacillus GG in their feces

Maija Saxelin; Tanja Pessi; Seppo Salminen

1995-01-01

161

Synergistic impact of Lactobacillus fermentum, Lactobacillus plantarum and vincristine on 1,2-dimethylhydrazine-induced colorectal carcinogenesis in mice  

PubMed Central

Lactobacillus sp. is the most dominant probiotic strain of bacteria. Evidence indicates that the consumption of Lactobacillus sp. reduces the risk of colorectal cancer in animal models. The present study was carried out to determine whether administration of Lactobacillus fermentum/ Lactobacillus plantarum alone or in combination with vincristine have a synergistic impact on the control of colorectal cancer in an animal model. Mice with 1,2 dimethylhydrazine (DMH) hydrochloride-induced colon cancer were fed with L. fermentum and L. plantarum isolated along with vincristine. An increase in body weight, a decrease in ammonia concentration, a decrease in ? glucosidase and ? glucuronidase enzyme activity and a reduction in the number of crypts in the mice in the pre-carcinogen-induced group was noted when compared to these variables in the post-carcinogen-induced group. The body weight of the mice fed L. fermentum along with vincristine was increased (6.5 g), and was found to be 3.5 times higher compared to that of the control. A marked decrease in the ammonia concentration (240 mg), and ? glucosidase (0.0023 IU) and ? glucopyranose enzyme activity (0.0027 IU) was observed; 22.59% less ammonia concentration, 73.26% less ? glucosidase activity and 56.46% less ? glucuronidase enzyme activity was noted when compared to the control. A significant reduction in the number of aberrant crypt foci (ACF) (90%) was observed when compared to the control. Maximum protection was observed in the mice fed the probiotics and vincristine prior to cancer induction. Among the different dietary combinations tested in the present study, L. fermentum and vincristine showed a more extensive reduction in ammonia concentration, ? glucosidase, ? glucuronidase activity and the number of ACF.

ASHA; GAYATHRI, DEVARAJA

2012-01-01

162

Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods  

Microsoft Academic Search

BACKGROUND: Comparative genomic hybridization (CGH) constitutes a powerful tool for identification and characterization of bacterial strains. In this study we have applied this technique for the characterization of a number of Lactobacillus strains isolated from the intestinal content of rats fed with a diet supplemented with sorbitol. RESULTS: Phylogenetic analysis based on 16S rRNA gene, recA, pheS, pyrG and tuf

Luz-Adriana Sarmiento-Rubiano; Bernard Berger; Déborah Moine; Manuel Zúñiga; Gaspar Pérez-Martínez; María J Yebra

2010-01-01

163

Synergistic impact of Lactobacillus fermentum, Lactobacillus plantarum and vincristine on 1,2-dimethylhydrazine-induced colorectal carcinogenesis in mice.  

PubMed

Lactobacillus sp. is the most dominant probiotic strain of bacteria. Evidence indicates that the consumption of Lactobacillus sp. reduces the risk of colorectal cancer in animal models. The present study was carried out to determine whether administration of Lactobacillus fermentum/ Lactobacillus plantarum alone or in combination with vincristine have a synergistic impact on the control of colorectal cancer in an animal model. Mice with 1,2 dimethylhydrazine (DMH) hydrochloride-induced colon cancer were fed with L. fermentum and L. plantarum isolated along with vincristine. An increase in body weight, a decrease in ammonia concentration, a decrease in ? glucosidase and ? glucuronidase enzyme activity and a reduction in the number of crypts in the mice in the pre-carcinogen-induced group was noted when compared to these variables in the post-carcinogen-induced group. The body weight of the mice fed L. fermentum along with vincristine was increased (6.5 g), and was found to be 3.5 times higher compared to that of the control. A marked decrease in the ammonia concentration (240 mg), and ? glucosidase (0.0023 IU) and ? glucopyranose enzyme activity (0.0027 IU) was observed; 22.59% less ammonia concentration, 73.26% less ? glucosidase activity and 56.46% less ? glucuronidase enzyme activity was noted when compared to the control. A significant reduction in the number of aberrant crypt foci (ACF) (90%) was observed when compared to the control. Maximum protection was observed in the mice fed the probiotics and vincristine prior to cancer induction. Among the different dietary combinations tested in the present study, L. fermentum and vincristine showed a more extensive reduction in ammonia concentration, ? glucosidase, ? glucuronidase activity and the number of ACF. PMID:22970015

Asha; Gayathri, Devaraja

2012-06-01

164

Characterization of a novel Lactobacillus species closely related to Lactobacillus johnsonii using a combination of molecular and comparative genomics methods  

PubMed Central

Background Comparative genomic hybridization (CGH) constitutes a powerful tool for identification and characterization of bacterial strains. In this study we have applied this technique for the characterization of a number of Lactobacillus strains isolated from the intestinal content of rats fed with a diet supplemented with sorbitol. Results Phylogenetic analysis based on 16S rRNA gene, recA, pheS, pyrG and tuf sequences identified five bacterial strains isolated from the intestinal content of rats as belonging to the recently described Lactobacillus taiwanensis species. DNA-DNA hybridization experiments confirmed that these five strains are distinct but closely related to Lactobacillus johnsonii and Lactobacillus gasseri. A whole genome DNA microarray designed for the probiotic L. johnsonii strain NCC533 was used for CGH analysis of L. johnsonii ATCC 33200T, L. johnsonii BL261, L. gasseri ATCC 33323T and L. taiwanensis BL263. In these experiments, the fluorescence ratio distributions obtained with L. taiwanensis and L. gasseri showed characteristic inter-species profiles. The percentage of conserved L. johnsonii NCC533 genes was about 83% in the L. johnsonii strains comparisons and decreased to 51% and 47% for L. taiwanensis and L. gasseri, respectively. These results confirmed the separate status of L. taiwanensis from L. johnsonii at the level of species, and also that L. taiwanensis is closer to L. johnsonii than L. gasseri is to L. johnsonii. Conclusion Conventional taxonomic analyses and microarray-based CGH analysis have been used for the identification and characterization of the newly species L. taiwanensis. The microarray-based CGH technology has been shown as a remarkable tool for the identification and fine discrimination between phylogenetically close species, and additionally provided insight into the adaptation of the strain L. taiwanensis BL263 to its ecological niche.

2010-01-01

165

Comparison of exopolysaccharide production by strains of Lactobacillus rhamnosus and Lactobacillus paracasei grown in chemically defined medium and milk  

Microsoft Academic Search

  Exopolysaccharide (EPS) production was compared among three strains of lactobacilli. Lactobacillus rhamnosus strain 9595M can be classified among the highest EPS-producing strains of lactic acid bacteria reported to date with a maximum\\u000a EPS production of 1275?mg?L?1. Under controlled pH, no significant differences in the quantity of EPS produced could be detected between carbon source\\u000a (glucose or lactose) or fermentation temperature

I Dupont; D Roy; G Lapointe

2000-01-01

166

The Barrier to HIV Transmission Provided by Genital Tract Lactobacillus Colonization.  

PubMed

While resistance to HIV transmission is due to multiple mechanisms such as the epithelium, a lower genital tract microbiota dominated by Lactobacillus appears to play an important role. This article reviews selected recent research on genital tract microbiota in women including how microbiota impacts HIV resistance and factors affecting Lactobacillus colonization. PMID:24661438

Mirmonsef, Paria; Spear, Gregory T

2014-06-01

167

Complete Genome Sequence of Lactobacillus helveticus R0052, a Commercial Probiotic Strain  

PubMed Central

Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found in Lactobacillus acidophilus.

Barreau, Guillaume; Broadbent, Jeffery R.

2012-01-01

168

Complete genome sequence of Lactobacillus helveticus R0052, a commercial probiotic strain.  

PubMed

Lactobacillus helveticus R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among L. helveticus strains in that it contained genes encoding mucus-binding proteins similar to those found in Lactobacillus acidophilus. PMID:23105080

Tompkins, Thomas A; Barreau, Guillaume; Broadbent, Jeffery R

2012-11-01

169

Whole-Genome Shotgun Sequencing of Lactobacillus rhamnosus MTCC 5462, a Strain with Probiotic Potential  

PubMed Central

Lactobacillus rhamnosus MTCC 5462 was isolated from infant gastrointestinal flora. The strain exhibited an ability to reduce cholesterol and stimulate immunity. The strain has exhibited positive results in alleviating gastrointestinal discomfort and good potential as a probiotic. We sequenced the whole genome of the strain and compared it to the published genome sequence of Lactobacillus rhamnosus GG (ATCC 53103).

Prajapati, J. B.; Khedkar, C. D.; Chitra, J.; Suja, Senan; Mishra, V.; Sreeja, V.; Patel, R. K.; Ahir, V. B.; Bhatt, V. D.; Sajnani, M. R.; Koringa, P. G.; Joshi, C. G.

2012-01-01

170

Adhesion of Lactobacillus species in urine and phosphate buffer to silicone rubber and glass under flow  

Microsoft Academic Search

Coating uroepithelial cells or catheter materials with lactobacilli has been shown to retard the development of a uropathogenic biofilm, with biosurfactant production and strong adhesion being two prerequisite properties of the Lactobacillus strains to be employed. In this paper, adhesion of six selected Lactobacillus strains to silicone rubber and glass in urine and in a phosphate buffer was studied using

K. W. Millsap; G. Reid; H. C. van der Mei; H. J. Busscher

1997-01-01

171

Removal of pathogenic bacteria and nitrogens by Lactobacillus spp. JK-8 and JK-11  

Microsoft Academic Search

The feasibility of using Lactobacillus strains for the simultaneous removal of pathogenic bacteria and nitrogens, with the ultimate aim of application for the improvement of water quality in shrimp farms, was explored. Initially, strains of Lactobacillus spp. JK-8 and JK-11 in shrimp ponds were screened for their ability to remove pathogenic bacteria and nitrogen compounds. At the end of the

Chae-Woo Ma; Yun-Seok Cho; Kye-Heon Oh

2009-01-01

172

Lactobacillus and bifidobacterium in irritable bowel syndrome: Symptom responses and relationship to cytokine profiles  

Microsoft Academic Search

Background & Aims: The aim of this study was to com- pare the response of symptoms and cytokine ratios in irritable bowel syndrome (IBS) with ingestion of probiotic preparations containing a lactobacillus or bifidobacterium strain. Methods: Seventy-seven subjects with IBS were ran- domized to receive either Lactobacillus salivarius UCC4331 or Bifidobacterium infantis 35624, each in a dose of 1 10

Liam O’Mahony; Jane McCarthy; Peter Kelly; George Hurley; Fangyi Luo; Kersang Chen; Gerald C. O’Sullivan; Barry Kiely; J. Kevin Collins; Fergus Shanahan; Eamonn M. M. Quigley

2005-01-01

173

Diversity and functional characterization of Lactobacillus spp. isolated throughout the ripening of a hard cheese.  

PubMed

The aim of this work was to study the Lactobacillus spp. intra- and inter- species diversity in a Piedmont hard cheese made of raw milk without thermal treatment and without addition of industrial starter, and to perform a first screening for potential functional properties. A total of 586 isolates were collected during the cheese production and identified by means of molecular methods: three hundred and four were identified as Lactobacillus rhamnosus, two hundred and forty as Lactobacillus helveticus, twenty six as Lactobacillus fermentum, eleven as Lactobacillus delbrueckii, three as Lactobacillus pontis, and two as Lactobacillus gasseri and Lactobacillus reuteri, respectively. A high genetic heterogeneity was detected by using the repetitive bacterial DNA element fingerprinting (rep-PCR) with the use of (GTG)5 primer resulting in eight clusters of L. helveticus and sixteen clusters in the case of L. rhamnosus. Most of isolates showed a high auto-aggregation property, low hydrophobicity values, and a general low survival to simulated digestion process. However, sixteen isolates showed promising functional characteristics. PMID:24819414

Bautista-Gallego, J; Alessandria, V; Fontana, M; Bisotti, S; Taricco, S; Dolci, P; Cocolin, L; Rantsiou, K

2014-07-01

174

Complete Genome Sequence of Lactobacillus delbrueckii subsp. bulgaricus Strain ND02?  

PubMed Central

Lactobacillus delbrueckii subsp. bulgaricus strain ND02 is a Chinese commercial dairy starter used for the manufacture of yoghurt. It was isolated from naturally fermented yak milk in Qinghai, China. Here, we report the main genome features of ND02 and several differences with two other published genomes of Lactobacillus delbrueckii subsp. bulgaricus strains.

Sun, Zhihong; Chen, Xia; Wang, Jicheng; Zhao, Wenjing; Shao, Yuyu; Guo, Zhuang; Zhang, Xingchang; Zhou, Zhemin; Sun, Tiansong; Wang, Lei; Meng, He; Zhang, Heping; Chen, Wei

2011-01-01

175

Genome Sequence of Lactobacillus plantarum EGD-AQ4, Isolated from Fermented Product of Northeast India.  

PubMed

We present a draft genome sequence of Lactobacillus plantarum strain EGD-AQ4, isolated from nonalcoholic fermented bamboo shoot products of Northeast India. The size of the draft genome sequence is the largest among all the reported genome sequences of Lactobacillus plantarum, thus enabling the exploration of new gene clusters involved in various functional and probiotic attributes. PMID:24407637

Qureshi, Asifa; Itankar, Yogeshwari; Ojha, Ramkrishna; Mandal, Manabendra; Khardenavis, Anshuman; Kapley, Atya; Purohit, Hemant J

2014-01-01

176

Genome Sequence of Lactobacillus plantarum EGD-AQ4, Isolated from Fermented Product of Northeast India  

PubMed Central

We present a draft genome sequence of Lactobacillus plantarum strain EGD-AQ4, isolated from nonalcoholic fermented bamboo shoot products of Northeast India. The size of the draft genome sequence is the largest among all the reported genome sequences of Lactobacillus plantarum, thus enabling the exploration of new gene clusters involved in various functional and probiotic attributes.

Itankar, Yogeshwari; Ojha, Ramkrishna; Mandal, Manabendra; Khardenavis, Anshuman; Kapley, Atya; Purohit, Hemant J.

2014-01-01

177

Proteolytic activity of Lactobacillus strains isolated from dryfermented sausages on muscle sarcoplasmic proteins  

Microsoft Academic Search

The proteolytic activity of seven strains of Lactobacillus from two species isolated from dry cured sausages was assayed using a soluble muscle extract as a source of proteins, at a temperature of 30 °C. The results indicated that the strains of Lactobacillus plantarum tested had the more active proteolytic system, showing the highest amino acid release in the medium after

Silvina Fadda; Graciela Vignolo; Aída P. R. Holgado; Guillermo Oliver

1998-01-01

178

Correlation between Morphological and Physiological Characters in the Classification of Members of the Genus Lactobacillus  

Microsoft Academic Search

SUMMARY : Physiologically definable groups within the genus Lactobacillus are morphologically distinct. There are marked differences between homofermentative and heterofermentative strains and between physiological groups within the former. In recent years little use has been made of morphology in the classification of Lactobacillus. Much emphasis has been placed upon the pleomorphism of members of the genus, a state of affairs

G. H. G. DAVIS; K. A. BISSET; C. M. F. HALE

1955-01-01

179

Lactobacillus delbrueckii : probable cause d’infections urinaires chez la femme très âgée  

Microsoft Academic Search

Lactobacillus urinary tract infection (UTI) seems exceptionally reported. Nevertheless, with the introduction of a chromogenic medium UriSelect 4, eight cases of UTI in old women (mean of 81.2 years) mediated by Lactobacillus delbrueckii identified by DNA sequencing were reported between 2007 and 2009.

M. Bernier; P. Njomnang Soh; A. Lochet; L. Prots; R. Félice; A. Senescau; R. Fabre; A. Philippon

2010-01-01

180

[Lactobacillus delbrueckii: probable agent of urinary tract infections in very old women].  

PubMed

Lactobacillus urinary tract infection (UTI) seems exceptionally reported. Nevertheless, with the introduction of a chromogenic medium UriSelect 4, eight cases of UTI in old women (mean of 81.2 years) mediated by Lactobacillus delbrueckii identified by DNA sequencing were reported between 2007 and 2009. PMID:20605373

Bernier, M; Njomnang Soh, P; Lochet, A; Prots, L; Félice, R; Senescau, A; Fabre, R; Philippon, A

2012-04-01

181

In vitro colonization ability of human colon mucosa by exogenous Lactobacillus strains  

Microsoft Academic Search

We have tested five Lactobacillus strains for their in vitro colonization ability in a human colon epithelium culture model. Positive colonization occurred in different degrees in the presence of four Lactobacillus strains. We have found that L. casei GG and L. acidophilus NCFB 1748 induce an intermediate colonization. These two strains, largely investigated by others, have been reported to be

Leila-ouassila Sarem-Damerdji; Farzin Sarem; Luc Marchal; Jean-Pierre Nicolas

1995-01-01

182

Genome Sequence of Lactobacillus fabifermentans Strain T30PCM01, Isolated from Fermenting Grape Marc  

PubMed Central

Here, we report the draft genome assembly of Lactobacillus fabifermentans strain T30PCM01 isolated from grape marc. Its genome is the largest (3.58 Mbp) among Lactobacillus species and reveals an enormous potential for carbohydrate utilization and transcriptional regulation.

Treu, Laura; Vendramin, Veronica; Bovo, Barbara; Giacomini, Alessio; Corich, Viviana

2014-01-01

183

Draft Genome Sequences of Two Lactobacillus Strains, L. farraginis JCM 14108T and L. composti JCM 14202T, Isolated from Compost of Distilled Sh?ch? Residue.  

PubMed

Here, we report the draft genome sequences of two type strains of Lactobacillus, Lactobacillus farraginis JCM 14108(T) and Lactobacillus composti JCM 14202(T), isolated from the compost of distilled sh?ch? residue. Their genome information will be useful for studies of ecological and physiological functions of these Lactobacillus species. PMID:24675866

Yuki, Masahiro; Oshima, Kenshiro; Suda, Wataru; Kitahara, Maki; Kitamura, Keiko; Iida, Toshiya; Hattori, Masahira; Ohkuma, Moriya

2014-01-01

184

Lactobacillus reuteri LA6 and Lactobacillus gasseri LA39 isolated from faeces of the same human infant produce identical cyclic bacteriocin  

Microsoft Academic Search

Reutericin 6, a bacteriocin produced by Lactobacillus reuteri LA6 that was isolated from the faeces of a human infant at 2 months of age, was purified to homogeneity from broth culture-supernatant by reverse-phase chromatography. Molecular weight (5652) by mass spectrometry and primary structure of reutericin 6 were identical to that of gassericin A produced by Lactobacillus gasseri LA39 which was

Yasushi Kawai; Yasuyuki Ishii; Koichiro Uemura; Haruki Kitazawa; Tadao Saito; Takatoshi Itoh

2001-01-01

185

Augmentation of antimicrobial metronidazole therapy of bacterial vaginosis with oral probiotic Lactobacillus rhamnosus GR1 and Lactobacillus reuteri RC14: randomized, double-blind, placebo controlled trial  

Microsoft Academic Search

This study enrolled 125 premenopausal women diagnosed with bacterial vaginosis (BV) by presence of vaginal irritation, discharge and ‘fishy’ odor, and Nugent criteria and detection of sialidase enzyme. The subjects were treated with oral metronidazole (500mg) twice daily from days 1 to 7, and randomized to receive oral Lactobacillus rhamnosus GR-1 (1×109) and Lactobacillus reuteri RC-14 (1×109) or placebo twice

Kingsley Anukam; Emmanuel Osazuwa; Ijeoma Ahonkhai; Michael Ngwu; Gibson Osemene; Andrew W. Bruce; Gregor Reid

2006-01-01

186

The control of Listeria innocua and Lactobacillus sakei in broth and meat slurry with the bacteriocinogenic strain Lactobacillus casei CRL705  

Microsoft Academic Search

The effect of the bacteriocin-producing strain, Lactobacillus casei CRL705, in the control of Listeria innocua 7 and Lactobacillus sakei CRL1424 in MRS medium and meat slurry during the storage under vacuum at chill temperatures was evaluated. L. sakei CRL 1424 isolated from vacuum-packaged contaminated raw meat was identified as the predominant indigenous lactic acid bacterial flora. Co-inoculation of MRS broth

Patricia H. Castellano; Wilhelm H. Holzapfel; Graciela M. Vignolo

2004-01-01

187

Characterization of reutericyclin produced by Lactobacillus reuteri LTH2584.  

PubMed

Lactobacillus reuteri LTH2584 exhibits antimicrobial activity that can be attributed neither to bacteriocins nor to the production of reuterin or organic acids. We have purified the active compound, named reutericyclin, to homogeneity and characterized its antimicrobial activity. Reutericyclin exhibited a broad inhibitory spectrum including Lactobacillus spp., Bacillus subtilis, B. cereus, Enterococcus faecalis, Staphylococcus aureus, and Listeria innocua. It did not affect the growth of gram-negative bacteria; however, the growth of lipopolysaccharide mutant strains of Escherichia coli was inhibited. Reutericyclin exhibited a bactericidal mode of action against Lactobacillus sanfranciscensis, Staphylococcus aureus, and B. subtilis and triggered the lysis of cells of L. sanfranciscensis in a dose-dependent manner. Germination of spores of B. subtilis was inhibited, but the spores remained unaffected under conditions that do not permit germination. The fatty acid supply of the growth media had a strong effect on reutericyclin production and its distribution between producer cells and the culture supernatant. Reutericyclin was purified from cell extracts and culture supernatant of L. reuteri LTH2584 cultures grown in mMRS by solvent extraction, gel filtration, RP-C(8) chromatography, and anion-exchange chromatography, followed by rechromatography by reversed-phase high-pressure liquid chromatography. Reutericyclin was characterized as a negatively charged, highly hydrophobic molecule with a molecular mass of 349 Da. Structural characterization (A. Höltzel, M. G. Gänzle, G. J. Nicholson, W. P. Hammes, and G. Jung, Angew. Chem. Int. Ed. 39:2766-2768, 2000) revealed that reutericyclin is a novel tetramic acid derivative. The inhibitory activity of culture supernatant of L. reuteri LTH2584 corresponded to that of purified as well as synthetic reutericyclin. PMID:11010877

Gänzle, M G; Höltzel, A; Walter, J; Jung, G; Hammes, W P

2000-10-01

188

Lactobacillus bacteremia associated with probiotic use in a pediatric patient with ulcerative colitis.  

PubMed

Probiotic strains of Lactobacillus are currently used in a variety of clinical practices with limited evidence to support their use. Lactobacillus species are a normal part of gastrointestinal flora, and bacteremia with probiotic strains of Lactobacillus is very uncommon. We describe a case of Lactobacillus bacteremia in a 17-year-old boy with ulcerative colitis managed with systemic corticosteroids and infliximab, who presented with fever to 102°F, flushing, and chills 1 week after starting Lactobacillus rhamnosus GG probiotics. Initial blood culture on day 2 of his fever was positive for Lactobacillus, however, subsequent blood cultures on day 3 and 5 were negative. He was treated empirically with antibiotics for 5 days and defervesced by day 8 of his illness. 16 S rRNA sequence analysis identified the organism from the patient's blood culture and probiotic capsule as L. rhamnosus with a 99.78% match for both the strains. This case report highlights the potential risk of Lactobacillus bacteremia in immunosuppressed patients with severe active ulcerative colitis. PMID:23426446

Vahabnezhad, Elaheh; Mochon, Albert Brian; Wozniak, Laura Joyce; Ziring, David Alexander

2013-01-01

189

B Cells Are Not Essential for Lactobacillus-Mediated Protection against Lethal Pneumovirus Infection.  

PubMed

We have shown previously that priming of respiratory mucosa with live Lactobacillus species promotes robust and prolonged survival from an otherwise lethal infection with pneumonia virus of mice, a property known as heterologous immunity. Lactobacillus priming results in a moderate reduction in virus recovery and a dramatic reduction in virus-induced proinflammatory cytokine production; the precise mechanisms underlying these findings remain to be elucidated. Because B cells have been shown to promote heterologous immunity against respiratory virus pathogens under similar conditions, in this study we explore the role of B cells in Lactobacillus-mediated protection against acute pneumovirus infection. We found that Lactobacillus-primed mice feature elevated levels of airway Igs IgG, IgA, and IgM and lung tissues with dense, B cell (B220(+))-enriched peribronchial and perivascular infiltrates with germinal centers consistent with descriptions of BALT. No B cells were detected in lung tissue of Lactobacillus-primed B cell deficient ?MT mice or Jh mice, and Lactobacillus-primed ?MT mice had no characteristic infiltrates or airway Igs. Nonetheless, we observed diminished virus recovery and profound suppression of virus-induced proinflammatory cytokines CCL2, IFN-?, and CXCL10 in both wild-type and Lactobacillus-primed ?MT mice. Furthermore, Lactobacillus plantarum-primed, B cell-deficient ?MT and Jh mice were fully protected from an otherwise lethal pneumonia virus of mice infection, as were their respective wild-types. We conclude that B cells are dispensable for Lactobacillus-mediated heterologous immunity and were not crucial for promoting survival in response to an otherwise lethal pneumovirus infection. PMID:24748495

Percopo, Caroline M; Dyer, Kimberly D; Garcia-Crespo, Katia E; Gabryszewski, Stanislaw J; Shaffer, Arthur L; Domachowske, Joseph B; Rosenberg, Helene F

2014-06-01

190

[Antagonistic properties of Lactobacillus plantarum strains, isolated from traditional fermented products of Ukraine].  

PubMed

The antagonistic activity of 109 lactobacillus strains, isolated from traditional fermented products of Ukraine, has been investigated and it has been shown that the significant part of strains show different levels of inhibition of opportunistic and phytopathogenic microorganisms. It has been shown that the antagonistic effect of Lactobacillus plantarum strains on the opportunistic and phytopathogenic microorganisms was dependent on the sources of Lactobacillus strains isolation. L. plantarum strains show a higher level of inhibition against phytopathogenic microorganisms than opportunistic test-strains. Eleven strains of L. plantarum demonstrated antagonistic activity for all used test-strains. PMID:25007440

Vasyliuk, O M; Kovalenko, N K; Harmasheva, I L

2014-01-01

191

Aglycone production by Lactobacillus rhamnosus CRL981 during soymilk fermentation.  

PubMed

Lactobacillus rhamnosus CRL981 showed the highest levels of beta-glucosidase and was selected to characterize this enzyme system, among 63 strains of different Lactobacillus species. The maximum activity was obtained at pH 6.4 and 42 degrees C. The enzyme showed weak resistance to thermal inactivation maintaining only 20% of the initial activity when it was exposed at 50 degrees C for 5 min. It also, showed stability when stored at 4 degrees C for 60 days. Afterwards, L. rhamnosus was evaluated for hydrolysis of isoflavones to aglycones, cell population, residual sugars and organic acid produced during fermentation on soymilk (37 degrees C for 24 h). Higher viable counts were obtained after 12 h of fermentation (8.85 log CFUml(-1)) followed by a drop of pH and an increase of acidity during fermentation due the production of organic acids. L. rhamnosus CRL981 was able to proliferate in soymilk and produce a high beta-glucosidase activity achieving a complete hydrolysis of glucoside isoflavones after 12 h of fermentation. The present study indicates that L. rhamnosus CRL981 could be used in the development of different aglycone-rich functional soy beverages. PMID:19269578

Marazza, Jose A; Garro, Marisa S; de Giori, Graciela Savoy

2009-05-01

192

Expression of bioactive porcine interferon-alpha in Lactobacillus casei.  

PubMed

In this study, we constructed an expression cassette containing the inducible lac promoter and the secretion signal from an S-layer protein of Lactobacillus brevis for the expression of porcine interferon-alpha (IFN-?) in Lactobacillus casei (Lb. casei). Reverse-transcriptase PCR verified the presence of porcine IFN-? mRNA in the recombinant Lb. casei. The porcine IFN-? protein expressed in the recombinant Lb. casei was identified by both Western blot analysis and ELISA. We used various pH values and induction times to optimize the yield of IFN-?, and found that induction with 0.8 % lactose for 16 h under anaerobic conditions produced the highest concentrations of IFN-?. Furthermore, the activity of porcine IFN-? in the cultural supernatant was evaluated on ST cells infected with pseudorabies virus. The results revealed that porcine IFN-? inhibited virus replication in vitro. The findings of our study indicate that recombinant Lb. casei producing porcine IFN-? has great potential for use as a novel oral antiviral agent in animal healthcare. PMID:24818858

Ma, Shi-Jie; Li, Kun; Li, Xin-Sheng; Guo, Xiao-Qing; Fu, Peng-Fei; Yang, Ming-Fan; Chen, Hong-Ying

2014-09-01

193

Lactobacillus prophylaxis for diarrhea due to enterotoxigenic Escherichia coli.  

PubMed

In vitro and animal experiments indicated that lactobacilli might prevent Escherichia coli from colonizing the intestine and may produce substances counteracting enterotoxin. Lactinex, a commercial preparation of dried Lactobacillus acidophilus and L. bulgaricus, is marketed for uncomplicated diarrhea. Preliminary experiments in nonfasting volunteers indicated that lactobacilli in this preparation colonized the small intestine for up to 6 h. To evaluate the protective efficacy of Lactinex, a double-blind randomized study was carried out in which 48 volunteers (23 receiving Lactinex and 25 receiving placebos) were challenged with E. coli strains that produced heat-stable or heat-labile enterotoxins or both. No significant differences between the two groups were noted with respect to attack rate, incubation period, duration of diarrhea, volume and number of liquid stools, and coproculture yields. These data suggest that this lactobacillus preparations does not prevent or alter the course of enterotoxigenic E. coli diarrhea in adults. Lack of efficacy occurred despite efforts to maximize small bowel colonization, including administration of Lactinex in milk and in a 6-hour-interval regimen during 36 h before and 96 h after challenge. PMID:6792978

Clements, M L; Levine, M M; Black, R E; Robins-Browne, R M; Cisneros, L A; Drusano, G L; Lanata, C F; Saah, A J

1981-07-01

194

Fatty Acid Composition of Bifidobacterium and Lactobacillus Strains  

PubMed Central

Normal C14, C16, and C18 saturated acids and C16 and C18 monoenoic acids are the main fatty acids of nine strains of Bifidobacterium. Their lactobacillic acid content was less than 5%. Lactobacillus strains contained the same fatty acids as main compounds except for octadecanoic acid, which was present only in very low amounts. Eight of nine Lactobacillus strains contained in the stationary phase more than 15% lactobacillic acid. No correlation was observed between the fatty acid composition and other physiological characteristics used in the literature for classification of strains of one genus. Aging of the culture, which involved a decrease of the pH, caused a lengthening of the chain length of the fatty acids of B. bifidum var. pennsylvanicus but only a conversion of octadecenoic to lactobacillic acid in the lactobacilli. Lowering of the temperature of cultivation decreased the chain length of the fatty acids of B. bifidum var. pennsylvanicus. L. lactis did not show any influence of the temperature on the chain length of the fatty acids. The percentage of unsaturated acids was temperature independent in both organisms.

Veerkamp, J. H.

1971-01-01

195

Lactobacillus acidophilus modulates the virulence of Clostridium difficile.  

PubMed

Clostridium difficile is a spore-forming, toxin-producing, anaerobic bacterium that colonizes the human gastrointestinal tract. This pathogen causes antibiotic-associated diarrhea and colitis in animals and humans. Antibiotic-associated diseases may be treated with probiotics, and interest is increasing in such uses of probiotics. This study investigated the effect of Lactobacillus strains on the quorum-sensing signals and toxin production of C. difficile. In addition, an in vivo experiment was designed to assess whether Lactobacillus acidophilus GP1B is able to control C. difficile-associated disease. Autoinducer-2 activity was measured for C. difficile using the Vibrio harveyi coupled bioluminescent assay. Cell extract (10?g/mL) of L. acidophilus GP1B exhibited the highest inhibitory activity among 5 to 40?g/mL cell-extract concentrations. Real-time PCR data indicated decreased transcriptional levels in luxS, tcdA, tcdB, and txeR genes in the presence of 10?g/mL of cell extract of L. acidophilus GP1B. Survival rates at 5d for mice given the pathogen alone with L. acidophilus GP1B cell extract or L. acidophilus GP1B were 10, 70, and 80%, respectively. In addition, the lactic acid-produced L. acidophilus GP1B exhibits an inhibitory effect against the growth of C. difficile. Both the L. acidophilus GP1B and GP1B cell extract have significant antipathogenic effects on C. difficile. PMID:24856984

Yun, B; Oh, S; Griffiths, M W

2014-08-01

196

Lactobacillus reuteri CRL 1098 and Lactobacillus acidophilus CRL 1014 differently reduce in vitro immunotoxic effect induced by Ochratoxin A.  

PubMed

Ochratoxin A (OTA) is a widespread mycotoxin contaminating several food products which causes detrimental health effects. The ability of Lactobacillus reuteri CRL 1098 and Lactobacillus acidophilus CRL 1014 to prevent OTA effects on TNF-? and IL-10 production and apoptosis induction in human peripheral blood mononuclear cells (PBMC) was investigated. Membrane rafts participation in these responses was also evaluated. L. reuteri reduced by 29% the OTA inhibition of TNF-? production whereas L. acidophilus increased 8 times the TNF-? production by OTA treated-PBMC. Also, both bacteria reversed apoptosis induced by OTA by 32%. However, neither of the bacteria reversed the OTA inhibition on IL-10 production. On the other hand, the lactobacilli were less effective to reverse OTA effects on disrupted-rafts PBMC. This study shows that two lactobacilli strains can reduce some negative OTA effects, being membrane rafts integrity necessary to obtain better results. Also, the results highlight the potential capacity of some lactobacilli strains usually included in natural dietary components in milk-derived products and cereals feed, to reduce OTA toxicity once ingested by humans or animals. PMID:22975144

Mechoud, Mónica A; Juarez, Guillermo E; de Valdez, Graciela Font; Rodriguez, Ana V

2012-12-01

197

Evaluation of Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM encapsulated using a novel impinging aerosol method in fruit food products.  

PubMed

This study investigated the effect of microencapsulation on the survival of Lactobacillus rhamnosus GG and Lactobacillus acidophilus NCFM and their acidification in orange juice at 25°C for nine days and at 4°C over thirty five days of storage. Alginate micro beads (10-40 ?m) containing the probiotics were produced by a novel dual aerosol method of alginate and CaCl(2) cross linking solution. Unencapsulated L. rhamnosus GG was found to have excellent survivability in orange juice at both temperatures. However unencapsulated L. acidophilus NCFM showed significant reduction in viability. Encapsulation of these two bacteria did not significantly enhance survivability but did reduce acidification at 25°C and 4°C. In agreement with this, encapsulation of L. rhamnosus GG also reduced acidification in pear and peach fruit-based foods at 25°C, however at 4°C difference in pH was insignificant between free and encapsulated cells. In conclusion, L. rhamnosus GG showed excellent survival in orange juice and microencapsulation has potential in reducing acidification and possible negative sensory effects of probiotics in orange juice and other fruit-based products. PMID:22633536

Sohail, Asma; Turner, Mark S; Prabawati, Elisabeth Kartika; Coombes, Allan G A; Bhandari, Bhesh

2012-07-01

198

Production of beta-glucosidase and hydrolysis of isoflavone phytoestrogens by Lactobacillus acidophilus, Bifidobacterium lactis, and Lactobacillus casei in soymilk.  

PubMed

The study determined beta-glucosidase activity of commercial probiotic organisms for hydrolysis of isoflavone to aglycones in fermenting soymilk. Soymilk made with soy protein isolate (SPI) was fermented with Lactobacillus acidophilus LAFTI L10, Bifidobacterium lactis LAFTI B94, and Lactobacillus casei LAFTI L26 at 37 degrees C for 48 h and the fermented soymilk was stored for 28 d at 4 degrees C. beta-Glucosidase activity of organisms was determined using rho-nitrophenyl beta-D-glucopyranoside as a substrate and the hydrolysis of isoflavone glycosides to aglycones by these organisms was carried out. The highest level of growth occurred at 12 h for L. casei L26, 24 h for B. lactis B94, and 36 h for L. acidophilus L10 during fermentation in soymilk. Survival after storage at 4 degrees C for 28 d was 20%, 15%, and 11% greater (P < 0.05) than initial cell counts, respectively. All the bacteria produced beta-glucosidase, which hydrolyzed isoflavone beta-glycosides to isoflavone aglycones. The decrease in the concentration of beta-glycosides and the increase in the concentration of aglycones were significant (P < 0.05) in the fermented soymilk. Increased isoflavone aglycone content in fermented soymilk is likely to improve the biological functionality of soymilk. PMID:18211356

Donkor, O N; Shah, N P

2008-01-01

199

Difference in Degradation Patterns on Inulin-type Fructans among Strains of Lactobacillus delbrueckii and Lactobacillus paracasei  

PubMed Central

Lactobacillus delbrueckii strains were assessed for their degradation patterns of various carbohydrates with specific reference to inulin-type fructans in comparison with those of Lactobacillus paracasei strains. Firstly, growth curves on glucose, fructose, sucrose and inulin-type fructans with increasing degrees of fructose polymerization (i.e., 1-kestose, fructo-oligosaccharides and inulin) of the strains were compared. L. paracasei DSM 20020 grew well on all these sugars, while the growth rates of the 4 L. delbrueckii strains were markedly higher on the fructans with a greater degree of polymerization than on fructose and sucrose. Secondly, sugar compositions of spent cultures of the strains of L. delbrueckii and L. paracasei grown in mMRS containing either the fructans or inulin were determined by thin layer chromatography, in which the spent cultures of L. paracasei DSM 20020 showed spots of short fructose and sucrose fractions, whereas those of the L. delbrueckii strains did not show such spots at all. These results suggest that, unlike the L. paracasei strains, the L. delbrueckii strains do not degrade the inulin-type fructans extracellularly, but transport the fructans capable of greater polymerization preferentially into their cells to be degraded intracellularly for their growth.

TSUJIKAWA, Yuji; NOMOTO, Ryohei; OSAWA, Ro

2013-01-01

200

Lactobacillus reuteri, A Member of the Gut Bacterial Flora: Studies on Antagonism, Metabolism and Genetics.  

National Technical Information Service (NTIS)

Strains of the genus Lactobacillus, isolated from pig intestine, were characterized with physiological tests and DNA/DNA hybridization. L. acidophilus and L. reuteri were the dominating species identified among these strains. Using a novel method, the lac...

L. Axelsson

1990-01-01

201

Heterologously Expressed Family 51 ?-l-Arabinofuranosidases from Oenococcus oeni and Lactobacillus brevis?  

PubMed Central

Putative ?-l-arabinofuranosidases of Oenococcus oeni and Lactobacillus brevis were heterologously expressed and characterized. We report the basic functional properties of the recombinant enzymes in comparison to those of a commercial family 51 arabinosidase of Aspergillus niger.

Michlmayr, Herbert; Schumann, Christina; Kulbe, Klaus D.; del Hierro, Andres M.

2011-01-01

202

Growth Response of Lactobacillus Casei (Atcc 7469) to Riboflavin, Fmn, and Fad.  

National Technical Information Service (NTIS)

The growth responses, measured turbidimetrically, of Lactobacillus casei (ATCC 7469) to riboflavin, flavin mononucleotide, and flavin adenine dinucleotide differ significantly from each other. In the assay of acid extracts of tissues for flavin content, t...

B. W. Langer S. Charoensiri

1966-01-01

203

Draft Genome Sequence of Lactobacillus plantarum Strain WJL, a Drosophila Gut Symbiont  

PubMed Central

Lactobacillus plantarum strain WJL, a member of the symbiotic gut bacteria, was isolated from the intestine of the fruit fly, Drosophila melanogaster. Here, we report the draft genome sequence of L. plantarum WJL.

Kim, Eun-Kyoung; Park, Young Min; Lee, Oun Young

2013-01-01

204

Rapid identification of Lactobacillus plantarum group using the SNaPshot minisequencing assay.  

PubMed

This study used SNaPshot minisequencing for species identification within the Lactobacillus plantarum group. A SNaPshot minisequencing assay using dnaK as a target gene was developed, and five SNP primers were designed by analysing the conserved regions of the dnaK sequences. The specificity of the minisequencing assay was evaluated using 35 strains of L. plantarum group species. The results showed that the SNaPshot minisequencing assay was able to unambiguously and simultaneously discriminate strains belonging to the species L. plantarum subsp. plantarum, L. plantarum subsp. argentoratensis, Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus fabifermentans. In conclusion, a rapid, accurate and cost-effective assay was successfully developed for species identification of the members of the L. plantarum group. PMID:21641139

Huang, Chien-Hsun; Chang, Mu-Tzu; Huang, Mu-Chiou; Lee, Fwu-Ling

2011-12-01

205

Conversion of D-Biotin to Biotin Vitamers by Lactobacillus Arabinosus.  

National Technical Information Service (NTIS)

Saccharomyces cerevisiae utilizes D-biotin, biotin sulfoxide, and several vitamers of biotin, whereas Lactobacillus arabinosus responds to biotin and biotin sulfoxide of the naturally occurring forms of the vitamin. The use of these organisms for differen...

J. Birnbaum H. C. Lichstein

1964-01-01

206

Draft Genome Sequence of Lactobacillus fermentum Lf1, an Indian Isolate of Human Gut Origin  

PubMed Central

Lactobacillus fermentum is a normal inhabitant of the human gastrointestinal tract. Here, we report the draft genome sequence of an Indian isolate of the probiotic strain L. fermentum Lf1, isolated from the human gut.

Sharma, Vineet K.; Mallapa, Rashmi H.; Batish, Virender K.

2013-01-01

207

Lactobacillus yonginensis sp. nov., a lactic acid bacterium with ginsenoside converting activity isolated from Kimchi.  

PubMed

A Gram-reaction-positive, non-motile, non-spore-forming, catalase-negative, facultatively anaerobic, rod-shaped, ?-glucosidase-producing lactic acid bacterium, designated strain THK-V8(T), was isolated from the Korean fermented food, Kimchi, and its taxonomic position was investigated by using a polyphasic approach. Strain THK-V8(T) was able to grow at 4-40 °C (optimum, 30 °C) and pH 4.0-7.0 (optimum, pH 6.0). Strain THK-V8(T) had the ability to transform ginsenoside Rb? to Rd. On the basis of 16S rRNA gene sequence similarity data, strain THK-V8(T) was shown to belong to the genus Lactobacillus. Strain THK-V8(T) was related to Lactobacillus koreensis DCY50(T) (98.8% sequence similarity), Lactobacillus parabrevis LMG 11984(T) (97.7%), Lactobacillus senmaizukei L13(T) (97.5%), Lactobacillus hammesii TMW1.1236(T) (97.3%) and Lactobacillus brevis ATCC 14687(T) (97.2%). Subsequently, sequence analysis of the RNA polymerase alpha subunit gene (rpoA) confirmed that strain THK-V8(T) showed a maximum rpoA gene sequence similarity value of 93% with Lactobacillus brevis LMG 6906(T). The G+C content of the genomic DNA was 47.8 mol%. The DNA-DNA hybridization values between strain THK-V8(T) and Lactobacillus parabrevis DCY50(T) and Lactobacillus parabrevis LMG 11984(T) were 46.1 ± 4.9% and 10.6 ± 2.9%, respectively. The major fatty acids were summed feature 7 (comprised of C(19:0) cyclo ?10c/19?6), C(14:0), C(16:0) and C(18:1)?9c. The cell wall peptidoglycan was of the A4? L-Lys-D-Asp type. The phenotypic and molecular properties indicated that strain THK-V8(T) represents a novel species within the genus Lactobacillus, for which the name Lactobacillus yonginensis sp. nov. is proposed. The type strain is THK-V8(T) (?=KACC 16236(T)?=JCM 18023(T)). PMID:23475345

Yi, Eun-Ji; Yang, Jung-Eun; Lee, Jung Min; Park, Yongjin; Park, Sang-Yong; Shin, Heon-Sub; Kook, MooChang; Yi, Tae-Hoo

2013-09-01

208

Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells  

Microsoft Academic Search

Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Seven Lactobacillus strains adhered well to the Caco-2 cells, of which three possessed calcium-independent adhesion properties. A high level of calcium-independent adhesion was observed with the human stool isolate Lactobacillus acidophilus strain LB. Scanning electron microscopy revealed that this strain adhered to

GILLES CHAUVIERE; M.-H. COCONNIER; SOPHIE KERNEIS; JACKY FOURNIAT; ALAIN L. SERVIN

1992-01-01

209

Characterization of a Mobile clpL Gene from Lactobacillus rhamnosus  

Microsoft Academic Search

Two genes encoding ClpL ATPase proteins were identified in a probiotic Lactobacillus rhamnosus strain, E-97800. Sequence analyses revealed that the genes, designated clpL1 and clpL2, share 80% identity. The clpL2 gene showed the highest degree of identity (98.5%) to a clpL gene from Lactobacillus plantarum WCFSI, while it was not detected in three other L. rhamnosus strains studied. According to

Aki Suokko; Kirsi Savijoki; Erja Malinen; Airi Palva; Pekka Varmanen

2005-01-01

210

The bactericidal effects of Lactobacillus acidophilus , garcinol and Protykin® compared to clarithromycin, on Helicobacter pylori  

Microsoft Academic Search

Chronic infection with Helicobacter pylori causes peptic ulcers, gastric cancer and lymphoma. We evaluated the inhibitory effects of the probiotic Lactobacillus acidophilus DDS-1J, the antibiotic clarithromycin and the natural antioxidants garcinol and Protykin® (containing 50% trans-resveratrol) on Helicobacter pylori strain ATCC 49503. The findings of this study indicate that Lactobacillus acidophilus DDS-1J exerts a growth inhibitory effect on H. pylori

Archana Chatterjee; Taharat Yasmin; Debasis Bagchi; Sidney J. Stohs

2003-01-01

211

Investigation into the Potential of Bacteriocinogenic Lactobacillus plantarum BFE 5092 for Biopreservation of Raw Turkey Meat  

Microsoft Academic Search

The bacteriocin-producing Lactobacillus plantarum BFE 5092 was assessed for its potential as a protective culture in the biopreservation of aerobically stored turkey meat.\\u000a This strain produces three bacteriocins, i.e. plantaricins EF, JK and N. The absolute expression of Lactobacillus plantarum BFE 5092 16S rRNA housekeeping gene, as well as l-ldh, plnEF and plnG genes as determined by quantitative, real-time-PCR, revealed

Gyu-Sung Cho; Alexander Hanak; Melanie Huch; Wilhelm H. Holzapfel; Charles M. A. P. Franz

2010-01-01

212

Genome Sequence of the Bacteriocin-Producing Lactobacillus curvatus Strain CRL705  

PubMed Central

Lactobacillus curvatus is one of the most prevalent lactic acid bacteria found in fermented meat products. Here, we present the draft genome sequence of Lactobacillus curvatus CRL705, a bacteriocin producer strain isolated from an Argentinean artisanal fermented sausage, which consists of 1,833,251 bp (GC content, 41.9%) and two circular plasmids of 12,342 bp (pRC12; GC, 43.9%) and 18,664 bp (pRC18; GC, 34.4%).

Hebert, Elvira Maria; Saavedra, Lucila; Taranto, Maria Pia; Mozzi, Fernanda; Magni, Christian; Nader, Maria Elena F.; Font de Valdez, Graciela; Sesma, Fernando; Vignolo, Graciela

2012-01-01

213

Mode of action of lactocin 705, a two-component bacteriocin from Lactobacillus casei CRL705  

Microsoft Academic Search

Lactocin 705 is a bacteriocin whose activity depends on the complementary action of two peptides (705? and 705?) of 33-amino-acid residues each and is produced by Lactobacillus casei CRL705. Biologically active, synthetic lactocin 705 was used to study the mode of action on sensitive cells of Lactobacillus plantarum CRL691. The addition of 90 nmol l?1 of lactocin 705 to cells

Patricia Castellano; Raul Raya; Graciela Vignolo

2003-01-01

214

In vitro activities of ketolide HMR3647, macrolides, and other antibiotics against Lactobacillus, Leuconostoc, and Pediococcus isolates.  

PubMed

Testing of susceptibility to 13 antibiotics was performed with 90 isolates of Lactobacillus, Leuconostoc, and Pediococcus. MICs at which 90% of the isolates tested were inhibited by HMR3647, erythromycin, and ciprofloxacin were 0.015, 0.125 and 32 microg/ml, respectively. The penicillin MIC was > or = 16 microg/ml against 26.2% of the studied Lactobacillus sp. isolates and 50% of Lactobacillus plantarum. HMR3647 showed excellent activity against these genera. PMID:10582908

Zarazaga, M; Sáenz, Y; Portillo, A; Tenorio, C; Ruiz-Larrea, F; Del Campo, R; Baquero, F; Torres, C

1999-12-01

215

Anaerobic sludge digestion in the presence of lactobacillus additive  

SciTech Connect

A laboratory evaluation of a lactobacillus fermentation product was performed to study its effects as an additive on the anaerobic digestion of sewage sludge under conventional and overloaded high-rate conditions. The overloaded conditions were those experienced in commercial municipal digesters. It was concluded from this work that the use of the additive at low concentrations permits digester operation at least up to double the loading of untreated digesters and at higher methane yields and volatile solids reductions without affecting effluent quality. The additive also imparts iproved digester stability and rapid response to loading rate and detention time excursions and upsets. The beneficial effets of the additive observed in the laboratory remain to be established with other feeds such as biomass, and in large-scale commercial digestion tests that are now in progress.

Ghosh, S.; Klass, D.L.

1980-01-01

216

Anaerobic sludge digestion with a biocatalytic additive. [Lactobacillus acidophilus  

SciTech Connect

Aimed at improving the process operating characteristics of anaerobic digestion for sludge stabilization and SNG production, this study evaluates the effects of a lactobacillus additive under normal, variable, and overload conditions. This whey fermentation product of an acid-tolerant strain of L. acidophilus fortified with CoCO/sub 3/, (NH/sub 4/)/sub 2/HPO/sub 4/, ferrous lactate, and lactic acid provides growth factors, metabolic intermediates, and enzymes needed for substrate degradation and cellular synthesis. Data indicate that the biochemical additive increases methane yield, gas production rate, and volatile solids reduction; decreases volatile acids accumulation; enhances the digester buffer capacity; and improves the fertilizer value and dewatering characteristics of the digested residue. Digester capacities could be potentially doubled when the feed is so treated. Results of field tests with six full-scale digesters confirm observations made with bench-scale digesters.

Ghosh, S.; Henry, M.P.; Fedde, P.A.

1982-01-01

217

Characterization of a Feruloyl Esterase from Lactobacillus plantarum  

PubMed Central

Lactobacillus plantarum is frequently found in the fermentation of plant-derived food products, where hydroxycinnamoyl esters are abundant. L. plantarum WCFS1 cultures were unable to hydrolyze hydroxycinnamoyl esters; however, cell extracts from the strain partially hydrolyze methyl ferulate and methyl p-coumarate. In order to discover whether the protein Lp_0796 is the enzyme responsible for this hydrolytic activity, it was recombinantly overproduced and enzymatically characterized. Lp_0796 is an esterase that, among other substrates, is able to efficiently hydrolyze the four model substrates for feruloyl esterases (methyl ferulate, methyl caffeate, methyl p-coumarate, and methyl sinapinate). A screening test for the detection of the gene encoding feruloyl esterase Lp_0796 revealed that it is generally present among L. plantarum strains. The present study constitutes the description of feruloyl esterase activity in L. plantarum and provides new insights into the metabolism of hydroxycinnamic compounds in this bacterial species.

Esteban-Torres, Maria; Reveron, Ines; Mancheno, Jose Miguel; de las Rivas, Blanca

2013-01-01

218

Lactobacillus rhamnosus GG as an Effective Probiotic for Murine Giardiasis  

PubMed Central

The gut microflora is an important constituent in the intestinal mucosal barrier and has been introduced as the concept of probiotic therapy that beneficially affects the host by improving its intestinal microbial balance. Therefore, the main objective of the study was to explore the protective potential of various lactobacilli strains for murine giardiasis. By experimentation, it was found that the probiotic supplementation of either Lactobacillus casei, L. acidophilus, L. plantarum, or L. rhamnosus GG, 7 days prior to inoculation with G. lamblia trophozoites, reduced the rate of cyst excretion compared with Giardia-infected mice. Interestingly, L. GG was found to be the most effective probiotic in reducing the duration of giardia cycle and acts as an effective prophylactic probiotic for murine giardiasis but needs to be clinically correlated due to entirely different human microflora.

Goyal, Nisha; Tiwari, Ram Prakash; Shukla, Geeta

2011-01-01

219

Composition of the Lactobacillus acidophilus complex isolated from vaginal flora.  

PubMed

One hundred and fifty human vaginal samples containing a diversity of pathogens or nonpathogens (Gardnerella vaginalis, Streptococcus sp., Staphylococcus sp., Candida albicans. Mycoplasma sp.) were examined for their content in lactobacilli of the Lactobacillus acidophilus complex. Although all samples contained lactobacilli, strains of the L. acidophilus complex were present in only twenty-nine cases. Isolates were further characterized and compared with type strains or reference strains in an attempt to differentiate by phenotypic means the genospecies of the L. acidophilus complex. Data regarding specific activities of beta-galactosidase (beta-gal) and of phospho-beta-galactosidase (P-beta-gal) provided no specific information at the species level within the L. acidophilus complex. DNA-relatedness differentiates this genospecies. Most lactobacilli isolated from the vaginal flora of symptomatic women were genotypically close to L. gasseri CIP 102991T by the technique of DNA/DNA hybridization. PMID:8722308

Lachlak, N; Ageron, E; Zampatti, O; Michel, G; Grimont, P A

1996-04-01

220

Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB.  

PubMed Central

The spent culture supernatant of the human Lactobacillus acidophilus strain LB produces an antibacterial activity against a wide range of gram-negative and gram-positive pathogens. It decreased the in vitro viability of Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Shigella flexneri, Escherichia coli, Klebsiella pneumoniae, Bacillus cereus, Pseudomonas aeruginosa, and Enterobacter spp. In contrast, it did not inhibit lactobacilli and bifidobacteria. The activity was heat stable and relatively sensitive to enzymatic treatments and developed under acidic conditions. The antimicrobial activity was independent of lactic acid production. Activity against S. typhimurium SL1344 infecting human cultured intestinal Caco-2 cells was observed as it was in the conventional C3H/He/oujco mouse model with S. typhimurium C5 infection and oral treatment with the LB spent culture supernatant.

Coconnier, M H; Lievin, V; Bernet-Camard, M F; Hudault, S; Servin, A L

1997-01-01

221

Functional analysis of the Lactobacillus casei BL23 sortases.  

PubMed

Sortases are a class of enzymes that anchor surface proteins to the cell wall of Gram-positive bacteria. Lactobacillus casei BL23 harbors four sortase genes, two belonging to class A (srtA1 and srtA2) and two belonging to class C (srtC1 and srtC2). Class C sortases were clustered with genes encoding their putative substrates that were homologous to the SpaEFG and SpaCBA proteins that encode mucus adhesive pili in Lactobacillus rhamnosus GG. Twenty-three genes encoding putative sortase substrates were identified in the L. casei BL23 genome with unknown (35%), enzymatic (30%), or adhesion-related (35%) functions. Strains disrupted in srtA1, srtA2, srtC1, and srtC2 and an srtA1 srtA2 double mutant were constructed. The transcription of all four sortase encoding genes was detected, but only the mutation of srtA1 resulted in a decrease in bacterial surface hydrophobicity. The ?-N-acetyl-glucosaminidase and cell wall proteinase activities of whole cells diminished in the srtA1 mutant and, to a greater extent, in the srtA1 srtA2 double mutant. Cell wall anchoring of the staphylococcal NucA reporter protein fused to a cell wall sorting sequence was also affected in the srtA mutants, and the percentages of adhesion to Caco-2 and HT-29 intestinal epithelial cells were reduced for the srtA1 srtA2 strain. Mutations in srtC1 or srtC2 result in an undetectable phenotype. Together, these results suggest that SrtA1 is the housekeeping sortase in L. casei BL23 and SrtA2 would carry out redundant or complementary functions that become evident when SrtA1 activity is absent. PMID:23042174

Muñoz-Provencio, Diego; Rodríguez-Díaz, Jesús; Collado, María Carmen; Langella, Philippe; Bermúdez-Humarán, Luis G; Monedero, Vicente

2012-12-01

222

Characterization of Two Virulent Phages of Lactobacillus plantarum  

PubMed Central

We characterized two Lactobacillus plantarum virulent siphophages, ATCC 8014-B1 (B1) and ATCC 8014-B2 (B2), previously isolated from corn silage and anaerobic sewage sludge, respectively. Phage B2 infected two of the eight L. plantarum strains tested, while phage B1 infected three. Phage adsorption was highly variable depending on the strain used. Phage defense systems were found in at least two L. plantarum strains, LMG9211 and WCSF1. The linear double-stranded DNA genome of the pac-type phage B1 had 38,002 bp, a G+C content of 47.6%, and 60 open reading frames (ORFs). Surprisingly, the phage B1 genome has 97% identity with that of Pediococcus damnosus phage clP1 and 77% identity with that of L. plantarum phage JL-1; these phages were isolated from sewage and cucumber fermentation, respectively. The double-stranded DNA (dsDNA) genome of the cos-type phage B2 had 80,618 bp, a G+C content of 36.9%, and 127 ORFs with similarities to those of Bacillus and Lactobacillus strains as well as phages. Some phage B2 genes were similar to ORFs from L. plantarum phage LP65 of the Myoviridae family. Additionally, 6 tRNAs were found in the phage B2 genome. Protein analysis revealed 13 (phage B1) and 9 (phage B2) structural proteins. To our knowledge, this is the first report describing such high identity between phage genomes infecting different genera of lactic acid bacteria.

Briggiler Marco, Mariangeles; Garneau, Josiane E.; Tremblay, Denise; Quiberoni, Andrea

2012-01-01

223

Amylolytic Lactobacillus strains from Bulgarian fermented beverage boza.  

PubMed

The lactic acid fermentation is a worldwide method for cereal processing. Great diversity of fermented foods and drinks is produced with the participation of amylolytic lactic acid bacteria (ALAB). In the present study the ALAB content of the Bulgarian cereal beverage "boza" was investigated. Two strains, Bom 816 and N3, were found to possess significant amylolytic activity. The strains' identification was based on genetic criteria, namely amplified ribosomal DNA restriction analysis (ARDRA) and sequencing of the 16S rDNA. The strain Bom 816 belongs to the species Lactobacillus plantarum and N3 to Lactobacillus pentosus, being the first amylolytic representative of this species. Optimization of the media composition with starch as a sole carbon source was done. The starch hydrolysis was most efficient in medium containing 4 g/l yeast and 8 g/l meat extracts. Thus, L. plantarum Bom 816 consumed 14 g/l starch, while L. pentosus N3 consumed 17 g/l. The highest values of lactic acid reached were 9.5 g/l produced by Bom 816 and 5.5 g/l produced by N3. In the presence of yeast extract L. pentosus N3 formed 0.8-1 g/l succinic acid. Both strains produced mainly cell-bound enzymes with amylase activity, at a pH optimum of 5.5, ranging from 3-4 to 21 U/ml for L. pentosus N3 and from 0.5 to 11.5 U/ml for L. plantarum Bom 816, in dependence of the assay conditions. PMID:20469641

Petrova, Penka; Emanuilova, Milena; Petrov, Kaloyan

2010-01-01

224

Tyrosine and phenylalanine catabolism by Lactobacillus cheese flavor adjuncts.  

PubMed

Bacterial metabolism of Tyr and Phe has been associated with the formation of aromatic compounds that impart barny-utensil and floral off-flavors in cheese. In an effort to identify possible mechanisms for the origin of these compounds in Cheddar cheese, we investigated Tyr and Phe catabolism by Lactobacillus casei and Lactobacillus helveticus cheese flavor adjuncts under simulated Cheddar cheese-ripening (pH 5.2, 4% NaCl, 15 degrees C, no sugar) conditions. Enzyme assays of cell-free extracts indicated that L. casei strains catabolize Tyr and Phe by successive, constitutively expressed transamination and dehydrogenation reactions. Similar results were obtained with L. helveticus strains, except that the dehydrogenase enzymes were induced during incubation under cheese-ripening conditions. Micellar electrokinetic capillary chromatography of supernatants from L. casei and L. helveticus strains incubated under simulated cheese-ripening conditions confirmed that Tyr and Phe transamination and dehydrogenation pathways were active in both species and also showed these reactions were reversible. Major products of Tyr catabolism were phydroxy phenyl lactic acid and p-hydroxy phenyl acetic acid, while Phe degradation gave rise to phenyl lactic acid, phenyl acetic acid, and benzoic acid. However, some of these products were likely formed by nonenzymatic processes, since spontaneous chemical degradation of the Tyr intermediate p-hydroxy phenyl pyruvic acid produced p-hydroxy phenyl acetic acid, p-hydroxy phenyl propionic acid, and p-hydroxy benzaldehyde, while chemical degradation of the Phe intermediate phenyl pyruvic acid gave rise to phenyl acetic acid, benzoic acid, phenethanol, phenyl propionic acid, and benzaldehyde. PMID:11384026

Gummalla, S; Broadbent, J R

2001-05-01

225

Lactobacillus reuteri-specific immunoregulatory gene rsiR modulates histamine production and immunomodulation by Lactobacillus reuteri.  

PubMed

Human microbiome-derived strains of Lactobacillus reuteri potently suppress proinflammatory cytokines like human tumor necrosis factor (TNF) by converting the amino acid l-histidine to the biogenic amine histamine. Histamine suppresses mitogen-activated protein (MAP) kinase activation and cytokine production by signaling via histamine receptor type 2 (H2) on myeloid cells. Investigations of the gene expression profiles of immunomodulatory L. reuteri ATCC PTA 6475 highlighted numerous genes that were highly expressed during the stationary phase of growth, when TNF suppression is most potent. One such gene was found to be a regulator of genes involved in histidine-histamine metabolism by this probiotic species. During the course of these studies, this gene was renamed the Lactobacillus reuteri-specific immunoregulatory (rsiR) gene. The rsiR gene is essential for human TNF suppression by L. reuteri and expression of the histidine decarboxylase (hdc) gene cluster on the L. reuteri chromosome. Inactivation of rsiR resulted in diminished TNF suppression in vitro and reduced anti-inflammatory effects in vivo in a trinitrobenzene sulfonic acid (TNBS)-induced mouse model of acute colitis. A L. reuteri strain lacking an intact rsiR gene was unable to suppress colitis and resulted in greater concentrations of serum amyloid A (SAA) in the bloodstream of affected animals. The PhdcAB promoter region targeted by rsiR was defined by reporter gene experiments. These studies support the presence of a regulatory gene, rsiR, which modulates the expression of a gene cluster known to mediate immunoregulation by probiotics at the transcriptional level. These findings may point the way toward new strategies for controlling gene expression in probiotics by dietary interventions or microbiome manipulation. PMID:24123819

Hemarajata, P; Gao, C; Pflughoeft, K J; Thomas, C M; Saulnier, D M; Spinler, J K; Versalovic, J

2013-12-01

226

Lactobacillus reuteri-Specific Immunoregulatory Gene rsiR Modulates Histamine Production and Immunomodulation by Lactobacillus reuteri  

PubMed Central

Human microbiome-derived strains of Lactobacillus reuteri potently suppress proinflammatory cytokines like human tumor necrosis factor (TNF) by converting the amino acid l-histidine to the biogenic amine histamine. Histamine suppresses mitogen-activated protein (MAP) kinase activation and cytokine production by signaling via histamine receptor type 2 (H2) on myeloid cells. Investigations of the gene expression profiles of immunomodulatory L. reuteri ATCC PTA 6475 highlighted numerous genes that were highly expressed during the stationary phase of growth, when TNF suppression is most potent. One such gene was found to be a regulator of genes involved in histidine-histamine metabolism by this probiotic species. During the course of these studies, this gene was renamed the Lactobacillus reuteri-specific immunoregulatory (rsiR) gene. The rsiR gene is essential for human TNF suppression by L. reuteri and expression of the histidine decarboxylase (hdc) gene cluster on the L. reuteri chromosome. Inactivation of rsiR resulted in diminished TNF suppression in vitro and reduced anti-inflammatory effects in vivo in a trinitrobenzene sulfonic acid (TNBS)-induced mouse model of acute colitis. A L. reuteri strain lacking an intact rsiR gene was unable to suppress colitis and resulted in greater concentrations of serum amyloid A (SAA) in the bloodstream of affected animals. The PhdcAB promoter region targeted by rsiR was defined by reporter gene experiments. These studies support the presence of a regulatory gene, rsiR, which modulates the expression of a gene cluster known to mediate immunoregulation by probiotics at the transcriptional level. These findings may point the way toward new strategies for controlling gene expression in probiotics by dietary interventions or microbiome manipulation.

Hemarajata, P.; Gao, C.; Pflughoeft, K. J.; Thomas, C. M.; Saulnier, D. M.; Spinler, J. K.

2013-01-01

227

Effect of lactobacillus in preventing post-antibiotic vulvovaginal candidiasis: a randomised controlled trial  

PubMed Central

Objective To test whether oral or vaginal lactobacillus can prevent vulvovaginitis after antibiotic treatment. Design Randomised, placebo controlled, double blind, factorial 2×2 trial. Setting Fifty general practices and 16 pharmacies in Melbourne, Australia. Participants Non-pregnant women aged 18-50 years who required a short course of oral antibiotics for a non-gynaecological infection: 278 were enrolled in the study, and results were available for 235. Interventions Lactobacillus preparations taken orally or vaginally, or both, from enrolment until four days after completion of their antibiotic course. Main outcome measures Participants' reports of symptoms of post-antibiotic vulvovaginitis, with microbiological evidence of candidiasis provided by a self obtained vaginal swab. Results Overall, 55/235 (23% (95% confidence interval 18% to 29%)) women developed post-antibiotic vulvovaginitis. Compared with placebo, the odds ratio for developing post-antibiotic vulvovaginitis with oral lactobacillus was 1.06 (95% confidence interval 0.58 to 1.94) and with vaginal lactobacillus 1.38 (0.75 to 2.54). Compliance with antibiotics and interventions was high. The trial was terminated after the second interim analysis because of lack of effect of the interventions. Given the data at this time, the chances of detecting a significant reduction in vulvovaginitis with oral or vaginal lactobacillus treatment were less than 0.032 and 0.0006 respectively if the trial proceeded to full enrolment. Conclusions The use of oral or vaginal forms of lactobacillus to prevent post-antibiotic vulvovaginitis is not supported by these results. Further research on this subject is unlikely to be fruitful, unless new understandings about the pathogenesis of post-antibiotic vulvovaginitis indicate a possible role for lactobacillus.

Pirotta, Marie; Gunn, Jane; Chondros, Patty; Grover, Sonia; O'Malley, Paula; Hurley, Susan; Garland, Suzanne

2004-01-01

228

The probiotic Lactobacillus acidophilus stimulates chloride/hydroxyl exchange activity in human intestinal epithelial cells.  

PubMed

Probiotics are viable nonpathogenic microorganisms that are considered to confer health benefits to the host. Recent studies indicated that some Lactobacillus species function as probiotics and have been used as alternative treatments for diarrhea, which occurs due to increased secretion, decreased absorption, or both. However, the direct effects of probiotics on intestinal electrolyte absorption are not known. Therefore, we examined the effects of Lactobacillus on luminal chloride/hydroxyl (Cl(-)/OH(-)) exchange activity in human intestinal epithelial cells. Postconfluent Caco-2 cells were treated with the Lactobacillus species Lactobacillus acidophilus (LA), Lactobacillus casei, Lactobacillus plantarum, or Lactobacillus rhamnosus (LR) for 3 h at a multiplicity of infection of 50. Cl(-)/OH(-) exchange activity was measured as 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid-sensitive (36)Cl uptake in base-loaded cells. Treatment with live, but not heat-killed, LA and LR significantly increased Cl(-)/OH(-) exchange activity (approximately 50%), whereas other species were ineffective. Similarly, the conditioned medium (supernatant) of live LA increased Cl(-)/OH(-) exchange. The ability of LA or its conditioned culture medium to enhance Cl(-)/OH(-) exchange activity was blocked by PI-3 kinase inhibition but was unaffected by inhibition of mitogen-activated protein kinases. Corresponding to the increased Cl(-)/OH(-) exchange activity, LA treatment increased the surface expression of the apical anion exchanger, SLC26A3 [Down Regulated in Adenoma (DRA)]. The increased DRA membrane localization might contribute to the increased Cl(-) absorption by LA. Our results suggest that LA secretes soluble effector molecule(s) into the culture medium that stimulate apical Cl(-)/OH(-) exchange activity via phosphatidylinositol-3 kinase mediated mechanism. PMID:18567760

Borthakur, Alip; Gill, Ravinder K; Tyagi, Sangeeta; Koutsouris, Athanasia; Alrefai, Waddah A; Hecht, Gail A; Ramaswamy, Krishnamurthy; Dudeja, Pradeep K

2008-07-01

229

Naturally Occurring Lactic Acid Bacteria Isolated from Tomato Pomace Silage  

PubMed Central

Silage making has become a significant method of forage conservation worldwide. To determine how tomato pomace (TP) may be used effectively as animal feed, it was ensilaged for 90 days and microbiology counts, fermentation characteristics and chemical composition of tomato pomace silage (TPS) were evaluated at the 30th, 60th, and 90th days, respectively. In addition, 103 lactic acid bacteria were isolated from TPS. Based on the phenotypic and chemotaxonomic characteristics, 16S rDNA sequence and carbohydrate fermentation tests, the isolates were identified as 17 species namely: Lactobacillus coryniformis subsp. torquens (0.97%), Lactobacillus pontis (0.97%), Lactobacillus hilgardii (0.97%), Lactobacillus pantheris (0.97%), Lactobacillus amylovorus (1.9%), Lactobacillus panis (1.9%), Lactobacillus vaginalis (1.9%), Lactobacillus rapi (1.9%), Lactobacillus buchneri (2.9%), Lactobacillus parafarraginis (2.9%), Lactobacillus helveticus (3.9%), Lactobacillus camelliae (3.9%), Lactobacillus fermentum (5.8%), Lactobacillus manihotivorans (6.8%), Lactobacillus plantarum (10.7%), Lactobacillus harbinensis (16.5%) and Lactobacillus paracasei subsp. paracasei (35.0%). This study has shown that TP can be well preserved for 90 days by ensilaging and that TPS is not only rich in essential nutrients, but that physiological and biochemical properties of the isolates could provide a platform for future design of lactic acid bacteria (LAB) inoculants aimed at improving the fermentation quality of silage.

Wu, Jing-jing; Du, Rui-ping; Gao, Min; Sui, Yao-qiang; Xiu, Lei; Wang, Xiao

2014-01-01

230

Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA Gene Sequence Analysis and Multiplex PCR Assay with recA Gene-Derived Primers  

Microsoft Academic Search

In this study, we succeeded in differentiating Lactobacillus plantarum, Lactobacillus pentosus, and Lactobacillus paraplantarum by means of recA gene sequence comparison. Short homologous regions of about 360 bp were amplified by PCR with degenerate consensus primers, sequenced, and analyzed, and 322 bp were considered for the inference of phylogenetic trees. Phylograms, obtained by parsimony, maximum likelihood, and analysis of data

SANDRA TORRIANI; GIOVANNA E. FELIS; FRANCO DELLAGLIO

2001-01-01

231

Identification of Lactobacillus strains of goose origin using MALDI-TOF mass spectrometry and 16S-23S rDNA intergenic spacer PCR analysis.  

PubMed

The objective of our study was to identify Lactobacillus sp. strains of goose origin using MALDI-TOF mass spectrometry, ITS-PCR and ITS-PCR/RFLP. All three techniques proved to be valuable tools for identification of avian lactobacilli and produced comparable classification results. Lactobacillus strains were isolated from 100% of geese aged 3 weeks to 4 years, but from only 25% of chicks aged 1-10 days. Among the 104 strains isolated, we distinguished 14 Lactobacillus species. The dominant species was Lactobacillus salivarius (35.6%), followed by Lactobacillus johnsonii (18.3%), Lactobacillus ingluviei (11.5%) and Lactobacillus agilis (7.7%). The intact-cell MALDI-TOF mass spectrometry enabled rapid species identification of the lactobacilli with minimal pretreatment. However, it produced more than one identification result for 11.5% examined strains (mainly of the species L. johnsonii). ITS-PCR distinguished 12 genotypes among the isolates, but was not able to differentiate closely related strains, i.e. between Lactobacillus amylovorus and Lactobacillus kitasatonis and between Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus zeae. These species were differentiated by ITS-PCR/RFLP using the restriction enzymes TaqI and MseI. The results obtained indicate that ITS-PCR and ITS-PCR/RFLP assays could be used not only for interspecific, but also for intraspecific, typing. PMID:24607713

Dec, Marta; Urban-Chmiel, Renata; Gnat, Sebastian; Puchalski, Andrzej; Wernicki, Andrzej

2014-04-01

232

Detection and identification of Lactobacillus bacteria found in the honey stomach of the giant honeybee Apis dorsata  

Microsoft Academic Search

This is the first assay that describes the isolation and identification of strains and species of Lactobacillus from the honey stomach of the Asiatic giant honeybee, Apis dorsata. Samples of honeybees were collected from A. dorsata colonies in different bee trees, and Lactobacillus was isolated from honey stomachs using selective media. The isolates were Gram-stained and tested for catalase reaction.

Naser Tajabadi; Makhdzir Mardan; Mohd Yazid Abdul Manap; Mustafa Shuhaimi; Amir Meimandipour; Leila Nateghi

233

Combination of Multiplex PCR and PCR-Denaturing Gradient Gel Electrophoresis for Monitoring Common Sourdough-Associated Lactobacillus Species  

PubMed Central

A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional Abruzzo (central Italy) sourdoughs.

Settanni, Luca; Valmorri, Sara; van Sinderen, Douwe; Suzzi, Giovanna; Paparella, Antonello; Corsetti, Aldo

2006-01-01

234

Whole Genome Sequence of the Probiotic Strain Lactobacillus paracasei N1115, Isolated from Traditional Chinese Fermented Milk  

PubMed Central

Lactobacillus paracasei N1115 is a new strain with probiotic properties isolated from traditional homemade dairy products in Inner Mongolia, China. Here, we report the complete genome sequence of L. paracasei N1115, which shows high similarity to the well-studied probiotic Lactobacillus rhamnosus GG, and 3 structures turned out to be inversions, according to the colinearity analysis of the BLAST alignment.

Wang, Shijie; He, Fang; Luo, Yongkang; Kang, Zhiyuan; Lu, Chun; Feng, Lili; Lu, Xiaoli; Xue, Yuling; Wang, Hua

2014-01-01

235

Quantitative Real-Time PCR Analysis of Fecal Lactobacillus Species in Infants Receiving a Prebiotic Infant Formula  

Microsoft Academic Search

The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predom- inate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can exert beneficial effects on, for example, diarrhea and atopy. However, the Lactobacillus species distribution in

Monique Haarman; Jan Knol

2006-01-01

236

Identification and functional characterization of Lactobacillus strains isolated from milk and Gioddu, a traditional Sardinian fermented milk  

Microsoft Academic Search

Raw milk from Sardinian cows and sheep, and sheep's milk yoghurt (Gioddu), are food products that are poorly characterized in terms of the presence of mesophilic Lactobacillus strains, and may be good sources from which to isolate novel bacterial strains. Sixteen Lactobacillus strains were identified by genotypic and phenotypic methods from samples of sheep's milk (n=7), fermented sheep's milk (n=7)

S. Ortu; G. E. Felis; M. Marzotto; A. Deriu; P. Molicotti; L. A. Sechi; F. Dellaglio; S. Zanetti

2007-01-01

237

Nutrient enrichment of cassava peels using a mixed culture of Saccharomyces cerevisae and Lactobacillus spp solid media fermentation techniques  

Microsoft Academic Search

Cassava pulp was fermented with pure strains of Saccharomyces cerevisae and two bacteria namely Lactobacillus delbruckii and Lactobacillus coryneformis for 3 days. The squeezed liquid from the fermented pulp was used to ferment cassava peels for 7 days. Analysis of the dried fermented peels revealed that there was a significant (P < 0.05) increase in the protein content of the

Ganiyu Oboh

2006-01-01

238

Adhesion of Lactobacillus species in urine and phosphate buffer to silicone rubber and glass under flow.  

PubMed

Coating uroepithelial cells or catheter materials with lactobacilli has been shown to retard the development of a uropathogenic biofilm, with biosurfactant production and strong adhesion being two prerequisite properties of the Lactobacillus strains to be employed. In this paper, adhesion of six selected Lactobacillus strains to silicone rubber and glass in urine and in a phosphate buffer was studied using a parallel plate flow chamber. In addition, adhesive cell surface properties of the lactobacilli, i.e. the pH dependences of their zeta potentials and their hydrophobicities by water contact angles, were determined. L. acidophilus ATCC 4356 and L. fermentum B54 were the only strains showing significant adhesion to both hydrophobic silicone rubber and hydrophilic glass, possibly by virtue of their high cell surface hydrophobicities (water contact angles of 68 and 75 degrees, respectively) and small zeta potentials (-10.0 and -8.1 mV in buffer, respectively). Both hydrophobic Lactobacillus strains adhered less well in urine than in buffer. The remaining Lactobacillus strains studied were hydrophilic, with water contact angles between 25 and 36 degrees, and had highly negative zeta potentials, reaching -37.7 mV in buffer. Adhesion of these highly negatively charged, hydrophilic strains in buffer was essentially absent, while for some of these strains minor adhesion in urine was observed. This study demonstrates that the adhesion of lactobacilli to substrata differs with strain hydrophobicity and charge, and that urinary components can affect the ability of hydrophilic Lactobacillus strains to adhere to substrata. PMID:9003903

Millsap, K W; Reid, G; van der Mei, H C; Busscher, H J

1997-01-01

239

Identification of Lactobacillus strains with probiotic features from the bottlenose dolphin (Tursiops truncatus)  

PubMed Central

Aims In order to develop complementary health management strategies for marine mammals, we used culture-based and culture-independent approaches to identify gastrointestinal lactobacilli of the common bottlenose dolphin, Tursiops truncatus. Methods and Results We screened 307 bacterial isolates from oral and rectal swabs, milk and gastric fluid, collected from 38 dolphins in the U.S. Navy Marine Mammal Program, for potentially beneficial features. We focused our search on lactobacilli and evaluated their ability to modulate TNF secretion by host cells and inhibit growth of pathogens. We recovered Lactobacillus salivarius strains which secreted factors that stimulated TNF production by human monocytoid cells. These Lact. salivarius isolates inhibited growth of selected marine mammal and human bacterial pathogens. In addition, we identified a novel Lactobacillus species by culture and direct sequencing with 96·3% 16S rDNA sequence similarity to Lactobacillus ceti. Conclusions Dolphin-derived Lact. salivarius isolates possess features making them candidate probiotics for clinical studies in marine mammals. Significance and Impact of the Study This is the first study to isolate lactobacilli from dolphins, including a novel Lactobacillus species and a new strain of Lact. salivarius, with potential for veterinary probiotic applications. The isolation and identification of novel Lactobacillus spp. and other indigenous microbes from bottlenose dolphins will enable the study of the biology of symbiotic members of the dolphin microbiota and facilitate the understanding of the microbiomes of these unique animals.

Diaz, MA; Bik, EM; Carlin, KP; Venn-Watson, SK; Jensen, ED; Jones, SE; Gaston, EP; Relman, DA; Versalovic, J

2013-01-01

240

Lactobacillus ghanensis sp. nov., a motile lactic acid bacterium isolated from Ghanaian cocoa fermentations.  

PubMed

Three Gram-positive, catalase-negative, motile, rod-shaped strains, designated L486, L489(T) and L499, were isolated from fermenting cocoa. These organisms produced DL-lactic acid from glucose without gas formation. Ammonia was not produced from arginine. Acid was produced from amygdalin, D-cellobiose, aesculin, D-fructose, D-glucose, D-galactose, D-mannitol, D-mannose, N-acetylglucosamine, L-rhamnose, sucrose, salicin and D-trehalose. The cell walls contained peptidoglycan of the d-meso-diaminopimelic acid type. A 16S rRNA gene sequence analysis revealed that the isolates belong phylogenetically to the genus Lactobacillus and are closely related to Lactobacillus nagelii, Lactobacillus vini and Lactobacillus satsumensis. Low DNA-DNA reassociation values were obtained between the isolates and the phylogenetically closest neighbours. On the basis of the genetic and phenotypic results, the isolates are considered to represent a novel species, for which the name Lactobacillus ghanensis is proposed. The type strain is L489(T) (=DSM 18630(T)=CCUG 53453(T)). PMID:17625177

Nielsen, Dennis S; Schillinger, Ulrich; Franz, Charles M A P; Bresciani, José; Amoa-Awua, Wisdom; Holzapfel, Wilhelm H; Jakobsen, Mogens

2007-07-01

241

Molecular detection of Lactobacillus species in the neovagina of male-to-female transsexual women  

PubMed Central

There is a general opinion that penile skin lined neovagina of transsexual women is not able to support the growth of lactobacilli. This study was undertaken to prove if lactobacilli strains could survive in neovagina and to characterise the most dominant Lactobacillus species. Sixty three male-to-female transsexual women without abnormal vaginal discharge, clinical signs of infection were recruited on an ongoing basis from among transsexual outpatients in an academic research institution and tertiary care centre. Neovaginal smears were taken for molecular Lactobacillus spp. profiling by denaturing gradient gel electrophoresis (PCR–DGGE). Lactobacillus species were detected from 47/63 transsexual women (75%). The 279 Lactobacillus signals detected by PCR-DGGE technique belonged to 13 different species. Lactobacilli of the L. delbrueckii group (L. gasseri, L. crispatus, L. johnsonii, L. iners, L. jensenii) were predominant. More than 90% of women harboured a combination of two or more neovaginal Lactobacillus species. In this study we report the frequent occurrence of lactobacilli from neovagina of transsexual women. Both, frequency and composition were similar to the normal lactic acid bacterial microflora in both women of reproductive age and postmenopausal women.

Petricevic, Ljubomir; Kaufmann, Ulrike; Domig, Konrad J.; Kraler, Manuel; Marschalek, Julian; Kneifel, Wolfgang; Kiss, Herbert

2014-01-01

242

Characterization of the most abundant Lactobacillus species in chicken gastrointestinal tract and potential use as probiotics for genetic engineering.  

PubMed

The count and diffusion of Lactobacilli species in the different gastrointestinal tract (GI) regions of broilers were investigated by quantitative real-time polymerase chain reaction, and the probiotic characteristics of six L. reuteri species isolated from broilers' GI tract were also investigated to obtain the potential target for genetic engineering. Lactobacilli had the highest diversity in the crop and the lowest one in the cecum. Compared with the lower GI tract, more Lactobacilli were found in the upper GI tract. Lactobacillus reuteri, L. johnsonii, L. acidophilus, L. crispatus, L. salivarius, and L. aviarius were the predominant Lactobacillus species and present throughout the GI tract of chickens. Lactobacillus reuteri was the most abundant Lactobacillus species. Lactobacillus reuteri XC1 had good probiotic characteristics that would be a potential and desirable target for genetic engineering. PMID:24850302

Wang, Lei; Fang, Mingjian; Hu, Yanping; Yang, Yuxin; Yang, Mingming; Chen, Yulin

2014-07-01

243

The life history of Lactobacillus acidophilus as a probiotic: a tale of revisionary taxonomy, misidentification and commercial success.  

PubMed

Lactobacillus acidophilus is a commercially significant bacterial probiotic, originally isolated from the human gastrointestinal tract and designated Bacillus acidophilus in 1900. Throughout the development of methods to identify and characterise bacteria, L. acidophilus has undergone multiple taxonomic revisions and is now the type species of a phylogenetic subgroup in the highly diverse and heterogeneous Lactobacillus genus. As a result of the limitations of differentiating phenotypically similar species by morphological and biochemical means and revisionary nature of Lactobacillus taxonomy, the characterisation of L. acidophilus has struggled with misidentification and misrepresentation. In contrast, due to its global use as a probiotic supplement in functional foods, L. acidophilus sensu stricto is now one of the most well-characterised Lactobacillus species. Here, we establish the provenance of L. acidophilus strains, unpicking historical and current misidentifications of L. acidophilus, and reviewing the probiotic, genomic and physiological characteristics of this important Lactobacillus species. PMID:24152174

Bull, Matthew; Plummer, Sue; Marchesi, Julian; Mahenthiralingam, Eshwar

2013-12-01

244

Lactobacillus casei potentiates induction of oral tolerance in experimental arthritis.  

PubMed

Probiotics have been shown to exert beneficial effects on modulation of diverse diseases. However, no information is available for the effect of probiotics in the induction of oral tolerance in autoimmune diseases. The main purpose of this study was to elucidate whether Lactobacillus casei (L. casei) affect the induction of oral tolerance in experimental rheumatoid arthritis (RA). Type II collagen (CII) alone or together with L. casei was orally administered into collagen-induced arthritis (CIA) rats, and its effects on the clinical and histopathological aspects of RA were investigated. Co-administration of L. casei with CII more effectively suppressed clinical symptoms, paw swelling, lymphocyte infiltration and destruction of cartilage tissues of experimental arthritis than the rats treated with CII alone. The enhanced therapeutic efficacy was associated with an increase in anti-inflammatory cytokines (IL-10 and TGF-beta) while decreasing pro-inflammatory cytokines (IL-1beta, IL-2, IL-6, IL-12, IL-17, IFN-gamma and TNF-alpha). Co-administration of L. casei with CII more effectively suppressed CII-reactive T cell proliferation and the levels of Th1-type IgG isotypes (IgG2a and IgG2b), while up-regulating Foxp3 expression levels and the population of Foxp3(+) CD4(+) T cells. Our study provides evidence that L. casei could potentiate antigen-specific oral tolerance and suppress Th1-type immune responses of arthritic inflammation. PMID:18804867

So, Jae-Seon; Lee, Choong-Gu; Kwon, Ho-Keun; Yi, Hwa-Jung; Chae, Chang-Suk; Park, Jin-A; Hwang, Ki-Chul; Im, Sin-Hyeog

2008-11-01

245

Characterization of genes involved in fructose utilization by Lactobacillus fermentum.  

PubMed

The genes encoding phosphoglucose isomerase (fruI) and fructokinase (fruK) of Lactobacillus fermentum NRRL-B-1932 were sequenced. They constituted an operon, which is involved in fructose metabolism of this strain by channeling intracellular fructose into the phosphoketolase pathway. A third open reading frame, unkR, upstream of the operon was identified as homologous to genes of LacI/GalR family repressors. The UnkR repressor's role in transcriptional control of the fruIK operon could, however, not be established by electrophoretic mobility shift assay (EMSA) analysis. Sequence analysis revealed two putative catabolite responsive elements (cre) in the promoter region of fruIK suggesting that the fruIK operon is under negative regulatory control by carbon catabolite repression. Expression and enzyme activity data were compatible with the assumption that the fruIK operon is repressed by glucose. No sugar specific phosphoenolpyruvate sugar transferase system activity for the transport of fructose, glucose, sucrose or mannose could be detected in L. fermentum NRRL-B-1932 cells, which suggest that fructose is taken up by a permease system. PMID:16741753

Helanto, Miia; Aarnikunnas, Johannes; Palva, Airi; Leisola, Matti; Nyyssölä, Antti

2006-07-01

246

Heterogeneity of S-layer proteins of Lactobacillus acidophilus strains.  

PubMed

An S-layer (surface regular array) was found in the cell wall from six out of ten strains of Lactobacillus acidophilus examined by electron microscopic observations. All of the six strains which were shown to carry the S-layers belonged to the deoxyribonucleic acid (DNA) homology group A, but not to B, which had been classified by Johnson et al (Int. J. Syst. Bacteriol. 30: 53-68, 1980). On the other hand, the other four strains which possessed no S-layers were in the homology group B. The apparent molecular weights of the S-layer proteins ranged from 41 to 49 kDa as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Of the S-layer proteins from the six strains, three were susceptible to chemical cleavage with N-chlorosuccinimide, giving different peptide maps. All of the six S-layer proteins were fragmented by limited proteolysis with Staphylococcus aureus V8 protease, and gave markedly different peptide patterns by the subsequent peptide mapping analysis, except that the peptide maps of the S-layer proteins from the two strains which were in the same subgroup were identical. PMID:1602999

Masuda, K

1992-01-01

247

Adhesion of Lactobacillus amylovorus to Insoluble and Derivatized Cornstarch Granules  

PubMed Central

Approximately 70% of the cells in a suspension of the amylolytic bacterium Lactobacillus amylovorus bind to cornstarch granules within 30 min at 25°C. More than 60% of the bound bacteria were removed by formaldehyde (2%) or glycine (1 M) at pH 2.0. More than 90% of the bound bacteria were removed by MgCl2 (2 M; pH 7.0). Binding of L. amylovorus to cornstarch was inhibited in heat-killed cells and in cells that had been pretreated with glutaraldehyde, formaldehyde, sodium azide, trypsin, or 1% soluble potato starch. Bacterial binding to cornstarch appeared to correlate with both the concentration of cornstarch in the suspension and the amylose content in the granules. The ability of L. amylovorus to adhere to cornstarch granules was reduced for granules that had been extracted with HCl-ethanol, HCl-methanol, HCl-propanol, or HCl-butanol. Chemical derivatization of cornstarch resulted in a wide variety of adhesion responses by these bacteria. For example, 2-O-butyl starch (degree of substitution, 0.09) enhanced adhesion, whereas two palmitate starches (degree of substitution, 0.48 and 0.09) exhibited reduced adhesion activities. 2-O-(2-hydroxybutyl) starch and starch-poly(ethylene-co-acrylic acid) ester showed adhesion activities similar to those of the nonderivatized starch controls. Images

Imam, Syed H.; Harry-O'Kuru, R. E.

1991-01-01

248

Timely approaches to identify probiotic species of the genus Lactobacillus  

PubMed Central

Over the past decades the use of probiotics in food has increased largely due to the manufacturer’s interest in placing “healthy” food on the market based on the consumer’s ambitions to live healthy. Due to this trend, health benefits of products containing probiotic strains such as lactobacilli are promoted and probiotic strains have been established in many different products with their numbers increasing steadily. Probiotics are used as starter cultures in dairy products such as cheese or yoghurts and in addition they are also utilized in non-dairy products such as fermented vegetables, fermented meat and pharmaceuticals, thereby, covering a large variety of products. To assure quality management, several pheno-, physico- and genotyping methods have been established to unambiguously identify probiotic lactobacilli. These methods are often specific enough to identify the probiotic strains at genus and species levels. However, the probiotic ability is often strain dependent and it is impossible to distinguish strains by basic microbiological methods. Therefore, this review aims to critically summarize and evaluate conventional identification methods for the genus Lactobacillus, complemented by techniques that are currently being developed.

2013-01-01

249

Functional genomics of Lactobacillus casei establishment in the gut.  

PubMed

Although the composition of the gut microbiota and its symbiotic contribution to key host physiological functions are well established, little is known as yet about the bacterial factors that account for this symbiosis. We selected Lactobacillus casei as a model microorganism to proceed to genomewide identification of the functions required for a symbiont to establish colonization in the gut. As a result of our recent development of a transposon-mutagenesis tool that overcomes the barrier that had prevented L. casei random mutagenesis, we developed a signature-tagged mutagenesis approach combining whole-genome reverse genetics using a set of tagged transposons and in vivo screening using the rabbit ligated ileal loop model. After sequencing transposon insertion sites in 9,250 random mutants, we assembled a library of 1,110 independent mutants, all disrupted in a different gene, that provides a representative view of the L. casei genome. By determining the relative quantity of each of the 1,110 mutants before and after the in vivo challenge, we identified a core of 47 L. casei genes necessary for its establishment in the gut. They are involved in housekeeping functions, metabolism (sugar, amino acids), cell wall biogenesis, and adaptation to environment. Hence we provide what is, to our knowledge, the first global functional genomics analysis of L. casei symbiosis. PMID:25024222

Licandro-Seraut, Hélène; Scornec, Hélène; Pédron, Thierry; Cavin, Jean-François; Sansonetti, Philippe J

2014-07-29

250

Lactobacillus rhamnosus as additive for maize and sorghum ensiling.  

PubMed

The effects of Lactobacillus rhamnosus AT195, a potential probiotic microorganism cultured in buffalo "scotta" whey, on chemical and microbiological composition in maize and sorghum ensiling were evaluated. Both crops were harvested, chopped, and treated or not with the selected strain prior to ensiling in fiberglass vertical silos; 90 days after ensiling, silages were sensorially evaluated and sampled. Different chemical components were evaluated both on fresh crops and silages: in particular, the water-soluble carbohydrates content was investigated by high-field NMR spectroscopy and the carbohydrate fermentation profile was performed by GC. Besides phenotypic identification and typing, microbiological studies included Lb. rhamnosus genotype typing by RAPD-PCR. All silages, inoculated or not, were well preserved, as their chemical and microbiological data along with the fermentation profiles showed. The selected strain used as inoculum influenced the lactic acid population of silages and evidenced a good survival performance during the ensiling process of both maize and sorghum. Moreover, the use of Lb. rhamnosus strain efficiently improved the quality of the multifactorial ensiling process by significantly reducing the ammonia nitrogen content of both maize and sorghum silages. PMID:17929890

Salimei, Elisabetta; Capilongo, Valeria; Simoni, Andrea; Peiretti, Pier Giorgio; Maglieri, Cristina; Romano, Cristina A; Mannina, Luisa; Coppola, Raffaele; Sorrentino, Elena

2007-11-14

251

Purification and Characterization of an Arginine Aminopeptidase from Lactobacillus sakei  

PubMed Central

An arginine aminopeptidase (EC 3.4.11.6) that exclusively hydrolyzes basic amino acids from the amino (N) termini of peptide substrates has been purified from Lactobacillus sakei. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps, which included hydrophobic interaction, gel filtration, and anion-exchange chromatography. This procedure resulted in a recovery rate of 4.2% and a 500-fold increase in specific activity. The aminopeptidase appeared to be a trimeric enzyme with a molecular mass of 180 kDa. The activity was optimal at pH 5.0 and 37°C. The enzyme was inhibited by sulfhydryl group reagents and several divalent cations (Cu2+, Hg2+, and Zn2+) but was activated by reducing agents, metal-chelating agents, and sodium chloride. The enzyme showed a preference for arginine at the N termini of aminoacyl derivatives and peptides. The Km values for Arg-7-amido-4-methylcoumarin (AMC) and Lys-AMC were 15.9 and 26.0 ?M, respectively. The nature of the amino acid residue at the C terminus of dipeptides has an effect on hydrolysis rates. The activity was maximal toward dipeptides with Arg, Lys, or Ala as the C-terminal residue. The properties of the purified enzyme, its potential function in the release of arginine, and its further metabolism are discussed because, as a whole, it could constitute a survival mechanism for L. sakei in the meat environment.

Sanz, Yolanda; Toldra, Fidel

2002-01-01

252

Biodiversity of Lactobacillus plantarum from traditional Italian wines.  

PubMed

In this study, 23 samples of traditional wines produced in Southern Italy were subjected to microbiological analyses with the aim to identify and biotype the predominant species of lactic acid bacilli. For this purpose, a multiple approach, consisting in the application of both phenotypic (API 50CHL test) and biomolecular methods (polymerase chain reaction-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing) was used. The results showed that Lactobacillus plantarum was the predominant species, whereas Lb. brevis was detected in lower amount. In detail, out of 80 isolates 58 were ascribable to Lb. plantarum and 22 to Lb. brevis. Randomly amplified polymorphic DNA-polymerase chain reaction was used to highlight intraspecific variability among Lb. plantarum strains. Interestingly, the cluster analysis evidenced a relationship between different biotypes of Lb. plantarum and their origin, in terms of wine variety. Data acquired in this work show the possibility to obtain several malolactic fermentation starter cultures, composed by different Lb. plantarum biotypes, for their proper use in winemaking processes which are distinctive for each wine. PMID:24817564

Testa, Bruno; Lombardi, Silvia Jane; Tremonte, Patrizio; Succi, Mariantonietta; Tipaldi, Luca; Pannella, Gianfranco; Sorrentino, Elena; Iorizzo, Massimo; Coppola, Raffaele

2014-08-01

253

Safety evaluation of Lactobacillus pentosus strain b240.  

PubMed

Lactobacillus pentosus has a long history of use in cooked and uncooked fermented foods. Viable and heat-killed nonviable preparations of L. pentosus strain b240 were evaluated for short term and subchronic toxicity and genotoxic potential. Dose levels were determined through acute oral toxicity tests with viable (LD(50)>2500 mg/kg) and nonviable (LD(50)>2000 mg/kg) b240. In the short term study, rats received 2500 mg/kg/day (?1.7×10(11)cfu/kg/day) viable b240 for 28 days. In the subchronic study, rats received 500, 1000 or 2000 mg/kg/day (up to ?3.0×10(12) cfu equivalents/kg/day) nonviable b240 for 91 days followed by a 28-day recovery. No mortalities occurred. No treatment-related effects were identified for general condition, body weight, food-water consumption, ophthalmology, urinalysis, hematology, blood chemistry, organ weights, histopathology and gross pathology. Although statistically significant effects were noted for several endpoints in the short term and subchronic studies, none were related to the test materials. The NOAEL for nonviable b240 was 2000 mg/kg/day, the highest dose tested. Additionally, nonviable b240 (? 5000 ?g/plate) was not mutagenic in Salmonella typhimurium or Escherichia coli tester strains nor did nonviable b240 orally administered to rats at levels ? 2000 mg/kg/day for two days, induce a clastogenic response. PMID:21056609

Szabo, Nancy J; Dolan, Laurie C; Burdock, George A; Shibano, Takashi; Sato, Shin-ichi; Suzuki, Hiroshi; Uesugi, Tohru; Yamahira, Satoko; Toba, Masamichi; Ueno, Hirofumi

2011-01-01

254

Adhesion properties of potentially probiotic Lactobacillus kefiri to gastrointestinal mucus.  

PubMed

We investigated the mucus-binding properties of aggregating and non-aggregating potentially probiotic strains of kefir-isolated Lactobacillus kefiri, using different substrates. All the strains were able to adhere to commercial gastric mucin (MUCIN) and extracted mucus from small intestine (SIM) and colon (CM). The extraction of surface proteins from bacteria using LiCl or NaOH significantly reduced the adhesion of three selected strains (CIDCA 8348, CIDCA 83115 and JCM 5818); although a significant proportion (up to 50%) of S-layer proteins were not completely eliminated after treatments. The surface (S-layer) protein extracts from all the strains of Lb. kefiri were capable of binding to MUCIN, SIM or CM, and no differences were observed among them. The addition of their own surface protein extract increased adhesion of CIDCA 8348 and 83115 to MUCIN and SIM, meanwhile no changes in adhesion were observed for JCM 5818. None of the seven sugars tested had the ability to inhibit the adhesion of whole bacteria to the three mucus extracts. Noteworthy, the degree of bacterial adhesion reached in the presence of their own surface protein (S-layer) extract decreased to basal levels in the presence of some sugars, suggesting an interaction between the added sugar and the surface proteins. In conclusion, the ability of these food-isolated bacteria to adhere to gastrointestinal mucus becomes an essential issue regarding the biotechnological potentiality of Lb. kefiri for the food industry. PMID:24168928

Carasi, Paula; Ambrosis, Nicolás M; De Antoni, Graciela L; Bressollier, Philippe; Urdaci, María C; Serradell, María de los Angeles

2014-02-01

255

Probiotic Attributes of Autochthonous Lactobacillus rhamnosus Strains of Human Origin.  

PubMed

The study was aimed at evaluating the probiotic potential of indigenous autochthonous Lactobacillus rhamnosus strains isolated from infant feces and vaginal mucosa of healthy female. The survival of the selected strains and the two reference strains (L. rhamnosus GG and L. casei Actimel) was 67-81 % at pH 2 and 70-80 % after passage through the simulated gastrointestinal fluid. These strains are able to grow in the presence of 4 % bile salt, 10 % NaCl, and 0.6 % phenol. The cell surface of L. rhamnosus strains is hydrophilic in nature as revealed by bacterial adhesion to hydrocarbons (BATH) assay. Despite this, L. rhamnosus strains showed mucin adherence, autoaggregation and coaggregation properties that are strain-specific. In addition, they produce bile salt hydrolase (BSH) and ?-galactosidase activities. L. rhamnosus strains exhibit antimicrobial activity against food spoilage organisms and gastrointestinal pathogens, as well as Candida and Aspergillus spp. L. rhamnosus strains have similar antibiotic susceptibility pattern, and resistance to certain antibiotics is intrinsic or innate. The strains are neither haemolytic nor producer of biogenic amines such as histamine, putrescine, cadaverine and tyramine. Lyophilized cells of L. rhamnosus Fb exhibited probiotic properties demonstrating potential of the strain for technological suitability and in the preparation of diverse probiotic food formulations. PMID:24682879

Pithva, Sheetal; Shekh, Satyamitra; Dave, Jayantilal; Vyas, Bharatkumar Rajiv Manuel

2014-05-01

256

Enteric coating of granules containing the probiotic Lactobacillus acidophilus.  

PubMed

Abstract In the present study, a capsule formulation composed of enteric coated granules of Lactobacillus acidophilus ATCC 4962 was developed using Eudragit L30D-55 as enteric polymer. Optimization of the capsule formulation was achieved with a maximum viable cell count after 2 h of incubation in acid medium and disintegration time of 1 h in buffer pH 6.8. The amount of Eudragit L30D-55 in the capsules correlated with gastric juice resistance. The best protective qualities against artificial gastric juice were observed when capsules were prepared from granules composed of L. acidophilus, corn starch, lactose monohydrate, polyvinylpyrrolidone and coated with 12.5 % (m/V) of Eudragit L30D-55. Capsule formulation of L. acidophilus in edible broth medium suspension serves as a cheap alternative to the expensive freeze-drying procedure for preparing L. acidophilus. In addition, the enteric coating using Eudragit L30D-55 could protect probiotics from the acidic gastric environment and enhance the bioactivity of probiotics along with replacement of pathogenic microbes in human intestine. PMID:24914724

Pyar, Hassan; Peh, Kok-Khiang

2014-06-01

257

Transfection of Lactobacillus bulgaricus protoplasts by bacteriophage DNA.  

PubMed Central

A protoplast transfection system has been developed for Lactobacillus bulgaricus. The procedure involves a polyethylene glycol-mediated fusion of bacteriophage DNA encapsulated in liposomes into mutanolysin-treated cells. With L. bulgaricus B004 and DNA isolated from the phage phi c5004, transfection reached a maximum when at least 95% of the cells were osmotically fragile. The incorporation of phage DNA into liposomes was essential; no transfectants were detected in the absence of liposomes. The largest number of transfectants was observed after longer periods (20 min) of fusion of mutanolysin-treated cells and liposomes with polyethylene glycol. The maximum efficiency of 5 x 10(7) PFU/microgram of DNA was reached after a 24-h incubation in growth media prior to plating transfected cells in an agar overlay to detect the appearance of plaques. A minimum of 4 h of incubation in growth medium after fusion was required to detect the production and release of virions. The possibility that the high frequencies observed were due to bursting of transfected cells and subsequent infection of additional cells was found not to be a factor. The number of transfectants observed was directly proportional to the quantity of DNA added. These results define conditions appropriate for the introduction of DNA into L. bulgaricus.

Boizet, B; Flickinger, J L; Chassy, B M

1988-01-01

258

Lactobacillus and Pediococcus species richness and relative abundance in the vagina of rhesus monkeys (Macaca mulatta)  

PubMed Central

Background The rhesus monkey is an important animal model to study human vaginal health to which lactic acid bacteria play a significant role. However, the vaginal lactic acid bacterial species richness and relative abundance in rhesus monkeys is largely unknown. Methods Vaginal swab samples were aseptically obtained from 200 reproductive aged female rhesus monkeys. Following Rogosa agar plating, single bacterial colonies representing different morphotypes were isolated and analyzed for whole-cell protein profile, species-specifc PCR, and 16S rRNA gene sequence. Results A total of 510 Lactobacillus strains of 17 species and one Pediococcus acidilactici were identified. The most abundant species was L. reuteri, which colonized the vaginas of 86% monkeys. L. johnsonii was the second most abundant species, which colonized 36% of monkeys. The majority of monkeys were colonized by multiple Lactobacillus species. Conclusions The vaginas of rhesus monkeys are frequently colonized by multiple Lactobacillus species, dominated by L. reuteri.

Gravett, Michael G.; Jin, Ling; Pavlova, Sylvia I.; Tao, Lin

2012-01-01

259

Inhibition of Neisseria gonorrhoeae Epithelial Cell Interactions by Vaginal Lactobacillus Species?  

PubMed Central

High levels of Lactobacillus, the dominant genus of the healthy human vaginal microbiota, have been epidemiologically linked to a reduced risk of infection following exposure to the sexually transmitted pathogen Neisseria gonorrhoeae. In this work, a cell culture model of gonococcal infection was adapted to examine the effects of lactobacilli on gonococcal interactions with endometrial epithelial cells in vitro. Precolonization of epithelial cells with Lactobacillus jensenii, Lactobacillus gasseri ATCC 33323, or L. gasseri ATCC 9857 reduced gonococcal adherence by nearly 50%. Lactobacilli also inhibited gonococcal invasion of epithelial cells by more than 60%, which was independent of the effect on adherence. Furthermore, lactobacilli were able to displace adherent gonococci from epithelial cells, suggesting that these organisms have potential as a postexposure prophylactic. Thus, vaginal lactobacilli have the ability to inhibit gonococci at two key steps of an infection, which might have a significant effect in determining whether the gonococcus will be able to successfully establish an infection following exposure in vivo.

Spurbeck, Rachel R.; Arvidson, Cindy Grove

2008-01-01

260

Free Glycogen in Vaginal Fluids Is Associated with Lactobacillus Colonization and Low Vaginal pH  

PubMed Central

Objective Lactobacillus dominates the lower genital tract microbiota of many women, producing a low vaginal pH, and is important for healthy pregnancy outcomes and protection against several sexually transmitted pathogens. Yet, factors that promote Lactobacillus remain poorly understood. We hypothesized that the amount of free glycogen in the lumen of the lower genital tract is an important determinant of Lactobacillus colonization and a low vaginal pH. Methods Free glycogen in lavage samples was quantified. Pyrosequencing of the 16S rRNA gene was used to identify microbiota from 21 African American women collected over 8–11 years. Results Free glycogen levels varied greatly between women and even in the same woman. Samples with the highest free glycogen had a corresponding median genital pH that was significantly lower (pH 4.4) than those with low glycogen (pH 5.8; p<0.001). The fraction of the microbiota consisting of Lactobacillus was highest in samples with high glycogen versus those with low glycogen (median?=?0.97 vs. 0.05, p<0.001). In multivariable analysis, having 1 vs. 0 male sexual partner in the past 6 months was negatively associated, while BMI ?30 was positively associated with glycogen. High concentrations of glycogen corresponded to higher levels of L. crispatus and L. jensenii, but not L. iners. Conclusion These findings show that free glycogen in genital fluid is associated with a genital microbiota dominated by Lactobacillus, suggesting glycogen is important for maintaining genital health. Treatments aimed at increasing genital free glycogen might impact Lactobacillus colonization.

Mirmonsef, Paria; Hotton, Anna L.; Gilbert, Douglas; Burgad, Derick; Landay, Alan; Weber, Kathleen M.; Cohen, Mardge; Ravel, Jacques; Spear, Gregory T.

2014-01-01

261

Lactobacillus brantae sp. nov., isolated from faeces of Canada geese (Branta canadensis).  

PubMed

Three strains of lactic acid bacteria (LAB) were isolated from the faeces of apparently healthy wild Canada geese (Branta canadensis) in 2010 by cultivating faecal LAB on Rogosa SL agar under aerobic conditions. These three isolates were found to share 99.9 % gene sequence similarity of their 16S rRNA, their 16S-23S intergenic transcribed spacer region (ITS), partial 23S rRNA, rpoB, rpoC, rpoA and pheS gene sequences. However, the three strains exhibited lower levels of sequence similarity of these genetic targets to all known LAB, and the phylogenetically closest species to the geese strains were Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus saniviri. In comparison to L. casei ATCC 393(T), L. paracasei ATCC 25302(T), L. rhamnosus ATCC 7469(T) and L. saniviri DSM 24301(T), the novel isolates reacted uniquely in tests for cellobiose, galactose, mannitol, citric acid, aesculin and dextrin, and gave negative results in tests for l-proline arylamidase and l-pyrrolydonyl-arylamidase, and in the Voges-Proskauer test. Biochemical tests for cellobiose, aesculin, galactose, gentiobiose, mannitol, melezitose, ribose, salicin, sucrose, trehalose, raffinose, turanose, amygdalin and arbutin could be used for differentiation between L. saniviri and the novel strains. On the basis of phenotypic and genotypic characteristics, and phylogenetic data, the three isolates represent a novel species of the genus Lactobacillus, for which the name Lactobacillus brantae sp. nov. is proposed. The type strain is SL1108(T) (= ATCC BAA-2142(T) = LMG 26001(T) = DSM 23927(T)) and two additional strains are SL1170 and SL60106. PMID:22021580

Volokhov, Dmitriy V; Amselle, Megan; Beck, Brian J; Popham, David L; Whittaker, Paul; Wang, Hua; Kerrigan, Elizabeth; Chizhikov, Vladimir E

2012-09-01

262

Crystal structure of an efficacious gonococcal adherence inhibitor: An enolase from Lactobacillus gasseri.  

PubMed

Enolases are highly conserved metalloenzymes ubiquitous to cellular metabolism. While these enzymes share a large degree of sequence and structural similarity, they have been shown to possess a wide range of moonlighting functions. Recent studies showed that an enolase from Lactobacillus gasseri impedes the ability of Neisseria gonorrhoeae to adhere to epithelial cells. We present the crystal structure of this enolase, the first from Lactobacillus, with one of its Mg(2+) cofactors. Determined using molecular replacement to 2.08Å, the structure has a flexible and surface exposed catalytic loop containing lysines, and may play a role in the inhibitory function. PMID:24859038

Raghunathan, Kannan; Harris, Paul T; Spurbeck, Rachel R; Arvidson, Cindy G; Arvidson, Dennis N

2014-06-27

263

Interferon induction in murine peritoneal macrophage by stimulation with Lactobacillus acidophilus.  

PubMed

Induction of interferon for a kind of dairy lactic acid bacteria, Lactobacillus acidophilus (L. acidophilus), was investigated in murine peritoneal macrophage (M phi) cultures. Lactobacillus acidophilus JCM 1034, 1132T, 1229 and 2125 induced IFN (12-34 I.U./ml) in M phi cultures in vitro. Strain 1132T- and 2125-induced IFNs were characterized as IFN alpha/beta by treatment with anti-IFNs serum. The results indicate that the inducing activity of IFNs may be one of the available biological parameters for designating the dairy products containing L. acidophilus as "physiologically functional foods." PMID:1603000

Kitazawa, H; Matsumura, K; Itoh, T; Yamaguchi, T

1992-01-01

264

Genome sequence of the bacteriocin-producing Lactobacillus curvatus strain CRL705.  

PubMed

Lactobacillus curvatus is one of the most prevalent lactic acid bacteria found in fermented meat products. Here, we present the draft genome sequence of Lactobacillus curvatus CRL705, a bacteriocin producer strain isolated from an Argentinean artisanal fermented sausage, which consists of 1,833,251 bp (GC content, 41.9%) and two circular plasmids of 12,342 bp (pRC12; GC, 43.9%) and 18,664 bp (pRC18; GC, 34.4%). PMID:22207745

Hebert, Elvira María; Saavedra, Lucila; Taranto, María Pía; Mozzi, Fernanda; Magni, Christian; Nader, María Elena F; Font de Valdez, Graciela; Sesma, Fernando; Vignolo, Graciela; Raya, Raúl R

2012-01-01

265

Mechanistic studies of ribonucleoside triphosphate reductase from Lactobacillus leichmannii  

SciTech Connect

The mechanism of action of the adenosylcobalamin (AdoCbl)-dependent ribonucleoside triphosphate reductase (RTPR) was investigated using isotope effect and substrate specificity studies. These experiments were conducted on RTPR purified by a new method from Lactobacillus leichmannii. Isotope effect studies using (3{prime}-{sup 3}H)UTP and (3{prime}-{sup 3}H)ATP demonstrated that the 3{prime} C-H bond of the nucleotide is cleaved in order to cleave the 2{prime} C-OH bond. AdoCbl does not act as a direct H abstractor from the 3{prime} position of the substrate, but instead is thought to act as a radical chain initiator to generate an amino acid radical on the enzyme. Further support for this enzyme mediated cleavage of the 3{prime} C-H bond of the nucleotide and the novel role of AdoCbl came from studies using (3{prime}{sup 3}H)2{prime}-chloro-2{prime}-deoxyuridine 5{prime}-triphosphate ((3{prime}-{sup 3}H)CIUTP). Evidence is presented that during the course of this reaction, the {sup 3}H abstracted from the 3{prime} position of (3{prime}-{sup 3}H)CIUTP was either exchanged with the solvent or returned to the {beta} face of the 2{prime} position to produce (2{prime}{sup 3}H)-2{prime}-deoxy-3{prime}-ketoUTP. This result demonstrates that RTPR is capable of catalyzing a rearrangement reaction. The significance of the RTPR-catalyzed rearrangement with respect to the AdoCbl-dependent enzymes which catalyze rearrangements is discussed.

Harris, G.M.

1984-01-01

266

Complete genome sequence of Lactobacillus plantarum WCFS1.  

PubMed

The 3,308,274-bp sequence of the chromosome of Lactobacillus plantarum strain WCFS1, a single colony isolate of strain NCIMB8826 that was originally isolated from human saliva, has been determined, and contains 3,052 predicted protein-encoding genes. Putative biological functions could be assigned to 2,120 (70%) of the predicted proteins. Consistent with the classification of L. plantarum as a facultative heterofermentative lactic acid bacterium, the genome encodes all enzymes required for the glycolysis and phosphoketolase pathways, all of which appear to belong to the class of potentially highly expressed genes in this organism, as was evident from the codon-adaptation index of individual genes. Moreover, L. plantarum encodes a large pyruvate-dissipating potential, leading to various end-products of fermentation. L. plantarum is a species that is encountered in many different environmental niches, and this flexible and adaptive behavior is reflected by the relatively large number of regulatory and transport functions, including 25 complete PTS sugar transport systems. Moreover, the chromosome encodes >200 extracellular proteins, many of which are predicted to be bound to the cell envelope. A large proportion of the genes encoding sugar transport and utilization, as well as genes encoding extracellular functions, appear to be clustered in a 600-kb region near the origin of replication. Many of these genes display deviation of nucleotide composition, consistent with a foreign origin. These findings suggest that these genes, which provide an important part of the interaction of L. plantarum with its environment, form a lifestyle adaptation region in the chromosome. PMID:12566566

Kleerebezem, Michiel; Boekhorst, Jos; van Kranenburg, Richard; Molenaar, Douwe; Kuipers, Oscar P; Leer, Rob; Tarchini, Renato; Peters, Sander A; Sandbrink, Hans M; Fiers, Mark W E J; Stiekema, Willem; Lankhorst, René M Klein; Bron, Peter A; Hoffer, Sally M; Groot, Masja N Nierop; Kerkhoven, Robert; de Vries, Maaike; Ursing, Björn; de Vos, Willem M; Siezen, Roland J

2003-02-18

267

Manganese and Defenses against Oxygen Toxicity in Lactobacillus plantarum  

PubMed Central

Lactobacillus plantarum is aerotolerant during log-phase growth on glucose, but is an obligate aerobe on polyols. Respiration was cyanide resistant and under certain conditions was associated with the accumulation of millimolar concentrations of H2O2. On glucose, optimal growth was observed in the absence of O2. Extracts of L. plantarum did not catalyze the reduction of paraquat by reduced nicotinamide adenine dinucleotide, but plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) was readily reduced. Such extracts produced O2? in the presence of NADH plus plumbagin. Plumbagin caused a 10-fold increase in the rate of respiration of intact cells in the presence of glucose and also imposed a loss of viability which was dependent upon both glucose and O2. Although extracts of L. plantarum were devoid of true superoxide dismutase activity, this organism was comparable to superoxide dismutase-containing species in its resistance toward hyperbaric O2 and toward the oxygen-dependent lethality of plumbagin. L. plantarum required Mn-rich media and actively accumulated Mn(II). Soluble extracts were found to contain approximately 9 ?g of Mn per mg of protein and 75 to 90% of this Mn was dialyzable. Such extracts exhibited a dialyzable and ethylenediaminetetraacetic acid-inhibitable ability to scavenge O2?. This O2?-scavenging activity was due to the dialyzable Mn(II) present in these extracts and could be mimicked by MnCl2. Cells grown in Mn-rich media were enriched in dialyzable Mn and were more resistant toward oxygen toxicity and toward the oxygen-dependent plumbagin toxicity than were cells grown in Mn-deficient media. L. plantarum exhibited no nutritional requirement for iron and little or no iron was present in these cells, even when they were grown in iron-rich media. L. plantarum thus appears to use millimolar levels of Mn(II) to scavenge O2?, much as most other organisms use micromolar levels of superoxide dismutases.

Archibald, Frederick S.; Fridovich, Irwin

1981-01-01

268

Uracil Salvage Pathway in Lactobacillus plantarum: Transcription and Genetic Studies  

PubMed Central

The uracil salvage pathway in Lactobacillus plantarum was demonstrated to be dependent on the upp-pyrP gene cluster. PyrP was the only high-affinity uracil transporter since a pyrP mutant no longer incorporated low concentrations of radioactively labeled uracil and had increased resistance to the toxic uracil analogue 5-fluorouracil. The upp gene encoded a uracil phosphoribosyltransferase (UPRT) enzyme catalyzing the conversion of uracil and 5-phosphoribosyl-?-1-pyrophosphate to UMP and pyrophosphate. Analysis of mutants revealed that UPRT is a major cell supplier of UMP synthesized from uracil provided by preformed nucleic acid degradation. In a mutant selection study, seven independent upp mutants were isolated and all were found to excrete low amounts of pyrimidines to the growth medium. Pyrimidine-dependent transcription regulation of the biosynthetic pyrimidine pyrR1-B-C-Aa1-Ab1-D-F-E operon was impaired in the upp mutants. Despite the fact that upp and pyrP are positioned next to each other on the chromosome, they are not cotranscribed. Whereas pyrP is expressed as a monocistronic message, the upp gene is part of the lp_2376-glyA-upp operon. The lp_2376 gene encodes a putative protein that belongs to the conserved protein family of translation modulators such as Sua5, YciO, and YrdC. The glyA gene encodes a putative hydroxymethyltransferase involved in C1 unit charging of tetrahydrofolate, which is required in the biosynthesis of thymidylate, pantothenate, and purines. Unlike upp transcription, pyrP transcription is regulated by exogenous pyrimidine availability, most likely by the same mechanism of transcription attenuation as that of the pyr operon.

Arsene-Ploetze, Florence; Nicoloff, Herve; Kammerer, Benoit; Martinussen, Jan; Bringel, Francoise

2006-01-01

269

LIFESTYLE OF LACTOBACILLUS PLANTARUM IN THE MOUSE CECUM  

PubMed Central

Summary Lactobacillus plantarum is a common inhabitant of mammalian gastrointestinal tracts. Strains of L. plantarum are also marketed as probiotics intended to confer beneficial health effects upon delivery to the human gut. To understand how L. plantarum adapts to its gut habitat, we used whole genome transcriptional profiling to characterize the transcriptome of strain WCFS1 during colonization of the ceca of adult germ-free C57Bl/6J mice fed a standard low-fat rodent chow diet rich in complex plant polysaccharides or a prototypic Western diet high in simple sugars and fat. L. plantarum colonized the digestive tracts of these animals to high levels, although L. plantarum was found in 10-fold higher amounts in the ceca of mice fed the standard chow. Metabolic reconstructions based on the transcriptional datasets revealed that genes involved in carbohydrate transport and metabolism form the principal functional group that is up-regulated in vivo compared to exponential phase cells grown in three different culture media, and that a Western diet provides a more nutritionally-restricted, growth limiting milieu for the microbe in the distal gut. A set of bacterial genes encoding cell surface-related functions were differentially regulated in both groups of mice. This set included down-regulated genes required for the D-alanylation of lipoteichoic acids, extracellular structures of L. plantarum that mediate interactions with the host immune system. These results, obtained in a reductionist gnotobiotic mouse model of the gut ecosystem, provide insights about the niches (professions) of this lactic acid bacterium, and a context for systematically testing features that affect epithelial and immune cell responses to this organism in the digestive tract.

Marco, Maria L.; Peters, Theodorus H.F.; Bongers, Roger S.; Molenaar, Douwe; van Hemert, Saskia; Sonnenburg, Justin L.; Gordon, Jeffrey I.; Kleerebezem, Michiel

2010-01-01

270

Two Arginine Repressors Regulate Arginine Biosynthesis in Lactobacillus plantarum  

PubMed Central

The repression of the carAB operon encoding carbamoyl phosphate synthase leads to Lactobacillus plantarum FB331 growth inhibition in the presence of arginine. This phenotype was used in a positive screening to select spontaneous mutants deregulated in the arginine biosynthesis pathway. Fourteen mutants were genetically characterized for constitutive arginine production. Mutations were located either in one of the arginine repressor genes (argR1 or argR2) present in L. plantarum or in a putative ARG operator in the intergenic region of the bipolar carAB-argCJBDF operons involved in arginine biosynthesis. Although the presence of two ArgR regulators is commonly found in gram-positive bacteria, only single arginine repressors have so far been well studied in Escherichia coli or Bacillus subtilis. In L. plantarum, arginine repression was abolished when ArgR1 or ArgR2 was mutated in the DNA binding domain, or in the oligomerization domain or when an A123D mutation occurred in ArgR1. A123, equivalent to the conserved residue A124 in E. coli ArgR involved in arginine binding, was different in the wild-type ArgR2. Thus, corepressor binding sites may be different in ArgR1 and ArgR2, which have only 35% identical residues. Other mutants harbored wild-type argR genes, and 20 mutants have lost their ability to grow in normal air without carbon dioxide enrichment; this revealed a link between arginine biosynthesis and a still-unknown CO2-dependent metabolic pathway. In many gram-positive bacteria, the expression and interaction of different ArgR-like proteins may imply a complex regulatory network in response to environmental stimuli.

Nicoloff, Herve; Arsene-Ploetze, Florence; Malandain, Cedric; Kleerebezem, Michiel; Bringel, Francoise

2004-01-01

271

Gastric emptying is involved in Lactobacillus colonisation in mouse stomach.  

PubMed

Lactobacilli are indigenous microbes of the stomach of rodents, with much lower numbers being present in mice fed a purified diet than in those fed a non-purified diet. We postulated that gastric emptying (GE) is responsible for the different colonisation levels of lactobacilli and tested this hypothesis in the present study. BALB/cCr Slc mice were fed either a non-purified diet or a purified diet for 2 weeks. The number of gastric tissue-associated lactobacilli was lower in mice fed the purified diet than in those fed the non-purified diet. GE, estimated by measuring the food recovered from the stomach, was higher in mice fed the purified diet than in those fed the non-purified diet and correlated negatively with the number of lactobacilli. Mice fed the non-purified diet exhibited lower GE rates even when lactobacilli were eliminated by ampicillin administration through the drinking-water, suggesting that GE is the cause but not the consequence of different Lactobacillus colonisation levels. The plasma concentrations of acylated ghrelin, a gastric hormone that promotes GE, were higher in mice fed the purified diet than in those fed the non-purified diet. There was a negative correlation between GE and the number of lactobacilli in mice fed the non-purified diet, the purified diet, and the purified diet supplemented with sugarbeet fibre (200 g/kg diet) or carboxymethyl cellulose (40 g/kg diet). We propose that a higher GE rate contributes, at least in part, to lower gastric colonisation levels of lactobacilli in mice fed a purified diet. PMID:24933648

Sahasakul, Yuraporn; Takemura, Naoki; Sonoyama, Kei

2014-08-01

272

Characterization of a cryptic plasmid from Lactobacillus fermentum KC5b and its use for constructing a stable Lactobacillus cloning vector  

Microsoft Academic Search

Lactobacillus fermentum KC5b, a strain originally isolated from the human vagina, contains a cryptic plasmid pKC5b. The sequence and genetic organization of the 4392-bp plasmid were determined. It contains two convergently oriented replicons, which are homologous to each other and to the stable replicon of the Enterococcus faecium plasmid pMBB1. The two replicons of pKC5b were used either individually or

Sylvia I. Pavlova; Ali O. Kiliç; Ljubisa Topisirovic; Natasa Miladinov; Catherine Hatzos; L. Tao

2002-01-01

273

Augmentation of antimicrobial metronidazole therapy of bacterial vaginosis with oral probiotic Lactobacillus rhamnosus GR-1 and Lactobacillus reuteri RC-14: randomized, double-blind, placebo controlled trial.  

PubMed

This study enrolled 125 premenopausal women diagnosed with bacterial vaginosis (BV) by presence of vaginal irritation, discharge and 'fishy' odor, and Nugent criteria and detection of sialidase enzyme. The subjects were treated with oral metronidazole (500 mg) twice daily from days 1 to 7, and randomized to receive oral Lactobacillus rhamnosus GR-1 (1 x 10(9)) and Lactobacillus reuteri RC-14 (1 x 10(9)) or placebo twice daily from days 1 to 30. Primary outcome was cure of BV as determined by normal Nugent score, negative sialidase test and no symptoms or signs of BV at day 30. A total of 106 subjects returned for 30-day follow-up, of which 88% were cured in the antibiotic/probiotic group compared to 40% in the antibiotic/placebo group (p<0.001). Of the remaining subjects, 30% subjects in the placebo group and none in the probiotic group had BV, while 30% in the placebo and 12% in the probiotic group fell into the intermediate category based upon Nugent score, sialidase result and clinical findings. High counts of Lactobacillus sp. (>10(5) CFU/ml) were recovered from the vagina of 96% probiotic-treated subjects compared to 53% controls at day 30. In summary, this study showed efficacious use of lactobacilli and antibiotic in the eradication of BV in black African women. PMID:16697231

Anukam, Kingsley; Osazuwa, Emmanuel; Ahonkhai, Ijeoma; Ngwu, Michael; Osemene, Gibson; Bruce, Andrew W; Reid, Gregor

2006-05-01

274

Effect of Lactobacillus pentosus S-PT84 ingestion on IFN-? production from plasmacytoid dendritic cells by virus stimulation.  

PubMed

We investigated the effect of ingesting Lactobacillus pentosus S-PT84 on the interferon-? (IFN-?) production from splenocytes and plasmacytoid dendritic cells by virus stimulation. IFN-? production by the Lactobacillus pentosus S-PT84 ingestion group was significantly greater under the virus-infected condition than that by the control group. Lactobacillus pentosus S-PT84 could enhance the production of IFN-? which is known as an important cytokine for preventing virus infection. It may therefore become a prophylactic tool against such virus infection. PMID:21307585

Izumo, Takayuki; Maekawa, Toshihiro; Ida, Masayuki; Kishi, Atsuko; Akatani, Kaoru; Kitagawa, Yoshinori; Kiso, Yoshinobu

2011-01-01

275

Deoxyribonucleic Acid Homology Among Lactobacillus Species of the Subgenus Strepto bacterium Orla-Jensen  

Microsoft Academic Search

The deoxyribonucleic acid (DNA) relationship among Lacto bacillus species of the subgenus Streptobacterium Orla-Jensen was assessed by means of DNA\\/ DNA hybridization experiments. High genetic homologies were found among Lactobacillus casei subsp. casei, L. cusei subsp. alactosus, L. cusei subsp. pseudoplantarum, and L. casei subsp. tolerans; however, there was only a low level of homology between the DNA of L.

F. DELLAGLIO; V. BOTTAZZI; MARISA VESCOVO

1975-01-01

276

Lactobacillus amylovorus, a new starch-hydrolyzing species from cattle waste-corn fermentations  

SciTech Connect

The morphology, physiology and fermentation characteristics of this hitherto unrecognized species are described. The new Lactobacillus species can be differentiated from L. acidophilus, L. jensenii, and L. leichmannii on the basis of starch fermentation, G + C content, vitamin requirements and stereoisomerism of lactic acid produced. The type strain of L. amylovorus is NRRL B-4540. (Refs. 39).

Nakamura, L.K.

1981-01-01

277

Efficacy of Lactobacillus GG in prevention of nosocomial diarrhea in infants  

Microsoft Academic Search

Objective: Nosocomial diarrhea is a major problem in pediatric hospitals worldwide. We evaluated the efficacy of orally administered Lactobacillus GG (LGG) in the prevention of this disease in young children. Study design: Eighty-one children aged 1 to 36 months who were hospitalized for reasons other than diarrhea were enrolled in a double-blind trial and randomly assigned at admission to receive

Hanna Szajewska; Maria Kotowska; Jacek Z. Mrukowicz; Malgorzata Armánska; Wieslaw Mikolajczyk

2001-01-01

278

Bile salts hydrolase plays a key role on cholesterol removal by Lactobacillus reuteri  

Microsoft Academic Search

The [ H]cholesterol removal by Lactobacillus reuteri was due to a co-precipitation together with unconjugated bile acids, which was linked to the bile salt hydrolase activity of the cells. No [ H]cholesterol was found inside the cells indicating that no assimilation occurred.

M. P. Taranto; F. Sesma; A. Pesce de Ruiz Holgado; G. F. de Valdez

1997-01-01

279

Safety and tolerance of Lactobacillus reuteri supplementation to a population infected with the human immunodeficiency virus  

Microsoft Academic Search

Probiotic supplementation may provide health benefits, especially for individuals with an underlying disease state that makes them more susceptible to infections. The purpose of this experiment was to evaluate the safety and tolerance of Lactobacillus reuteri ingestion by subjects infected with the human immunodeficiency virus (HIV). Thirty-nine subjects consumed a freeze-dried preparation of L. reuteri or a placebo for 21

B. W. Wolf; K. B. Wheeler; D. G. Ataya; K. A. Garleb

1998-01-01

280

Contribution of reutericyclin production to the stable persistence of Lactobacillus reuteri in an industrial sourdough fermentation  

Microsoft Academic Search

Reutericyclin is a small molecular weight antibiotic produced by the sourdough isolate Lactobacillus reuteri LTH2584. This strain was isolated from an industrial sourdough, SER, in 1988. To determine whether reutericyclin formation contributes to the stable persistence of L. reuteri in sourdough, evaluations were made on whether reutericyclin-producing strains were among L. reuteri isolates from the SER sourdough obtained in 1994

Michael G Gänzle; Rudi F Vogel

2003-01-01

281

Genomic and Genetic Characterization of the Bile Stress Response of Probiotic Lactobacillus reuteri ATCC 55730  

Microsoft Academic Search

Probiotic bacteria encounter various stresses after ingestion by the host, including exposure to the low pH in the stomach and bile in the small intestine. The probiotic microorganism Lactobacillus reuteri ATCC 55730 has previously been shown to survive in the human small intestine. To address how L. reuteri can resist bile stress, we performed microarray experiments to determine gene expression

Kristi Whitehead; James Versalovic; Stefan Roos; Robert A. Britton

2008-01-01

282

The complete coenzyme B12 biosynthesis gene cluster of Lactobacillus reuteri CRL 1098  

Microsoft Academic Search

The coenzyme B12 production pathway in Lactobacillus reuteri has been deduced using a combination of genetic, biochemical and bioinformatics approaches. The coenzyme B12 gene cluster of Lb. reuteri CRL1098 has the unique feature of clustering together the cbi, cob and hem genes. It consists of 29 ORFs encoding the complete enzymic machinery necessary for de novo biosynthesis. Transcriptional analysis showed

Filipe Santos; Jose L. Vera; Renevan der Heijden; Graciela Valdez; Vos de W. M; Fernando Sesma; Jeroen Hugenholtz

2008-01-01

283

A collagen binding protein from Lactobacillus reuteri is part of an ABC transporter system?  

Microsoft Academic Search

The gene coding for a collagen binding protein from Lactobacillus reuteri NCIB 11951 was cloned and sequenced. A genomic lambda library was constructed and recombinant plaques were screened using antisera raised against purified collagen binding proteins from the same L. reuteri strain. The positive plaques were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis, which revealed the

Stefan Roos; Pär Aleljung; Normand Robert; Byong Lee; Torkel Wadström; Martin Lindberg; Hans Jonsson

1996-01-01

284

A natural compound (reuterin) produced by Lactobacillus reuteri for biological-tissue fixation  

Microsoft Academic Search

The study was undertaken to examine the degree of tissue fixation by reuterin, a natural compound produced by Lactobacillus reuteri, at distinct fixation conditions (pH, temperature, and fixative concentration). Additionally, the rate of tissue fixation by reuterin was investigated using glutaraldehyde as a control. It was found by the Fourier transformed infrared spectroscopy and nuclear magnetic resonance spectroscopy that both

Hsing-Wen Sung; Chiun-Nan Chen; Hsiang-Fa Liang; Min-Hao Hong

2003-01-01

285

Colonization and Immunomodulation by Lactobacillus reuteri ATCC 55730 in the Human Gastrointestinal Tract  

Microsoft Academic Search

Lactobacillus reuteri ATCC 55730 is a probiotic (health-promoting) bacterium widely used as a dietary supplement. This study was designed to examine local colonization of the human gastrointestinal mucosa after dietary supplementation with L. reuteri ATCC 55730 and to determine subsequent immune responses at the colonized sites. In this open clinical investigation, 10 healthy volunteers and 9 volunteers with ileostomy underwent

Nana Valeur; Peter Engel; Noris Carbajal; Eamonn Connolly; Karin Ladefoged

2004-01-01

286

Isolation of histamine-producing Lactobacillus buchneri from Swiss cheese implicated in a food poisoning outbreak.  

PubMed Central

A histamine-producing strain of Lactobacillus buchneri was isolated from Swiss cheese that had been implicated in an outbreak of histamine poisoning. It produced up to 4,070 nmol of histamine per ml in MRS broth supplemented with 0.1% histidine. The identification of this isolate was based on its biochemical, bacteriological, and DNA characterizations.

Sumner, S S; Speckhard, M W; Somers, E B; Taylor, S L

1985-01-01

287

Genome Sequence of the Cheese-Starter Strain Lactobacillus delbrueckii subsp. lactis CRL 581.  

PubMed

We report the genome sequence of Lactobacillus delbrueckii subsp. lactis CRL 581 (1,911,137 bp, GC 49.7%), a proteolytic strain isolated from a homemade Argentinian hard cheese which has a key role in bacterial nutrition and releases bioactive health-beneficial peptides from milk proteins. PMID:23929489

Hebert, Elvira María; Raya, Raúl R; Brown, Lucía; Font de Valdez, Graciela; Savoy de Giori, Graciela; Taranto, María Pía

2013-01-01

288

Effects of a Lactobacillus casei Synbiotic on Serum Lipoprotein, Intestinal Microflora, and Organic Acids in Rats  

Microsoft Academic Search

The main aim of this study was to evaluate the effec- tiveness of 3 synbiotic diets: 1) containing Lactobacillus casei ASCC 292 and fructooligosaccharides (LF diet); 2) containing L. casei ASCC 292 and maltodextrin (LM diet); and 3) containing L. casei ASCC 292, fructooligo- saccharide, and maltodextrin (LFM diet) to reduce se- rum cholesterol in male Wistar rats. The effect

M. T. Liong; N. P. Shah

2006-01-01

289

Hop-resistant Lactobacillus brevis contains a novel plasmid harboring a multidrug resistance-like gene  

Microsoft Academic Search

The bitter-tasting compounds derived from the flowers of the hop plant (Humulus lupulus L.) protect beer from bacterial spoilage. However, a few lactic acid bacteria, especially lactobacilli, are resistant to these compounds and sometimes cause serious spoilage in the beer industry. It is important to elucidate the mechanisms of hop-resistance in lactic acid bacteria. We selected mutants of Lactobacillus brevis

Manabu Sami; Hiroshi Yamashita; Tatsuhiko Hirono; Hiroshi Kadokura; Katsuhiko Kitamoto; Koji Yoda; Makari Yamasaki

1997-01-01

290

Cloning of a novel specific SCAR marker for species identification in Lactobacillus pentosus.  

PubMed

Identifying Lactobacillus species using only phenotypic and genotypic (16S rDNA sequence analysis) techniques yields inaccurate results. The objective of this study was to develop species-specific primers based on randomly amplified polymorphic DNA (RAPD) fingerprinting to distinguish species within the closely related Lactobacillus plantarum group. One of these primers, OPD-3, produced a species-specific band that was found only in the tested Lactobacillus pentosus. This specific fragment was isolated from agarose gel and ligated into a vector for DNA sequencing. A pair of primers, SpOPD3Lpen-F1/R1, that were highly specific sequence-characterized-amplified-regions (SCARs) were designed according to the nucleotide sequences of the specific RAPD marker. These primers were used for PCR analysis of the template DNA of the Lactobacillus strains, and a single 542 bp species-specific band was found only in L. pentosus. Using PCR, a novel species-specific primer pair is shown to rapidly, accurately and effectively distinguish L. pentosus from other species in the L. plantarum group of probiotic bacteria. PMID:24675147

Huang, Chien-Hsun; Chang, Mu-Tzu; Huang, Lina

2014-08-01

291

Isolation and characterization of a Lactobacillus plantarum bacteriophage, ?JL-1, from a cucumber fermentation  

Microsoft Academic Search

A virulent Lactobacillus plantarum bacteriophage, ?JL-1, was isolated from a commercial cucumber fermentation. The phage was specific for two related strains of L. plantarum, BI7 and its mutant (deficient in malolactate fermenting ability) MU45, which have been evaluated as starter cultures for controlled cucumber fermentation and as biocontrol microorganisms for minimally processed vegetable products. The phage genome of ?JL-1 was

Z. Lu; F. Breidt Jr; H. P. Fleminga; E. Altermann; T. R. Klaenhammer

2003-01-01

292

Catheter-related bacteremia due to Lactobacillus rhamnosus in a single-lung transplant recipient.  

PubMed

There is still a major debate about the pathogenicity of Lactobacillus spp. and some reports emphasize that these microorganisms are never isolated from endovascular devices. In this report we present a case of catheter-related bacteremia due to L. rhamnosus in a patient who underwent a single-lung transplant. PMID:11728051

Carretto, E; Barbarini, D; Marzani, F C; Fumagalli, P; Monzillo, V; Marone, P; Emmi, V

2001-01-01

293

Enhancement of lactic acid production with ram horn peptone by Lactobacillus casei  

Microsoft Academic Search

Ram horns are a waste material from the meat industry. The use of ram horn peptone (RHP) as a supplement for lactic acid production was investigated using Lactobacillus casei. For this purpose, first, RHP was produced. Ram horns were hydrolysed by treating with acids (3 M H2SO4 and 6 M HCl) and neutralizing the solutions to yield ram horn hydrolysate

Esabi Basaran Kurbanoglu

2004-01-01

294

Genome Sequence of Lactobacillus ingluviei, a Bacterium Associated with Weight Gain in Animals  

PubMed Central

We report the draft genome sequence of Lactobacillus ingluviei strain Autruche 4 (CSURP209) isolated from an ostrich. L. ingluviei is associated with weight gain in mice. This genome sequence may help us understand the obesity-induced mechanisms of intestinal bacteria.

Merhej, Vicky; Armougom, Fabrice; Robert, Catherine

2012-01-01

295

2-DE and MS analysis of interactions between Lactobacillus fermentum I5007 and intestinal epithelial cells.  

PubMed

Lactobacillus is a probiotic commonly used for supplementation to human and animal diets. In this study, we used 2-DE and MS to analyze changes in the proteomes of Lactobacillus and intestinal epithelial cells in two model systems. The in vivo and in vitro models were involved the inoculation of Lactobacillus fermentum I5007 into the rabbit jejunum for 4 h and the culture of the bacterium with Caco-2 cells for 1 h, respectively. Our results indicate that, after exposure to the intestinal environment, the bacterium exhibited decreases in key enzymes involved in energy metabolism (e.g., lactate dehydrogenase, dihydrolipoamide dehydrogenase, and nicotinate phosphoribosyltransferase) and amino acid metabolism (e.g., arginyl-tRNA synthetase and aspartate-semialdehyde dehydrogenase), but increases in glycoside hydrolase (an enzyme for mucin degradation) and fructose-6-phosphate phosphoketolase (an enzyme of the pentose phosphate pathway). In response to an interaction with L. fermentum I5007, Caco-2 cells showed changes in proteins that were beneficial for gut integrity, including voltage-dependent anion channel 1, glutathione transferase, and heat shock protein gp96. On the basis of their functions, we suggest that these proteins serve as useful biomarkers for metabolic changes in Lactobacillus and intestinal epithelial cells in response to their interactions. PMID:18004711

Yang, Fang; Wang, Junjun; Li, Xiaojie; Ying, Tianyi; Qiao, Shiyan; Li, Defa; Wu, Guoyao

2007-12-01

296

Complete Genome Sequences of Lactobacillus Phages J-1 and PL-1  

PubMed Central

Lactobacillus phages J-1 and PL-1 were isolated during the 1960s from abnormal fermentations of Yakult. The genomes are almost identical, but PL-1 has a deletion in the genetic switch region and also differs in a gene coding for a putative tail protein.

Dieterle, Maria Eugenia; Jacobs-Sera, Deborah; Russell, Daniel; Hatfull, Graham

2014-01-01

297

Lactobacillus rodentium sp. nov., from the digestive tract of wild rodents.  

PubMed

Three strains of regular, long, Gram-stain-positive bacterial rods were isolated using TPY, M.R.S. and Rogosa agar under anaerobic conditions from the digestive tract of wild mice (Mus musculus). All 16S rRNA gene sequences of these isolates were most similar to sequences of Lactobacillus gasseri ATCC 33323(T) and Lactobacillus johnsonii ATCC 33200(T) (97.3?% and 97.2?% sequence similarities, respectively). The novel strains shared 99.2-99.6?% 16S rRNA gene sequence similarities. Type strains of L. gasseri and L. johnsonii were also most related to the newly isolated strains according to rpoA (83.9-84.0?% similarities), pheS (84.6-87.8?%), atpA (86.2-87.7?%), hsp60 (89.4-90.4?%) and tuf (92.7-93.6?%) gene sequence similarities. Phylogenetic studies based on 16S rRNA, hsp60, rpoA, atpA and pheS gene sequences, other genotypic and many phenotypic characteristics (results of API 50 CHL, Rapid ID 32A and API ZYM biochemical tests; cellular fatty acid profiles; cellular polar lipid profiles; end products of glucose fermentation) showed that these bacterial strains represent a novel species within the genus Lactobacillus. The name Lactobacillus rodentium sp. nov. is proposed to accommodate this group of new isolates. The type strain is MYMRS/TLU1(T) (?=?DSM 24759(T)?=?CCM 7945(T)). PMID:24478214

Killer, J; Havlík, J; Vlková, E; Rada, V; Pechar, R; Benada, O; Kope?ny, J; Kofro?ova, O; Sechovcová, H

2014-05-01

298

Bifidobacterium spp. and Lactobacillus acidophilus: biological, biochemical, technological and therapeutical properties relevant for use as probiotics  

Microsoft Academic Search

This review focuses on the biological properties and consequent technological roles of intestinal bacteria with potential health-promoting capacities, and provides selected examples available in the literature that are pertinent to the aforementioned concepts. A comprehensive overview pertaining to the taxonomy and ecology, as well as nutritional and health effects of Bifidobacterium spp. and Lactobacillus acidophilus, is provided; particular attention is

Ana M. P. Gomes; F. Xavier Malcata

1999-01-01

299

Selective detection, enumeration and identification of potentially probiotic Lactobacillus and Bifidobacterium species in mixed bacterial populations  

Microsoft Academic Search

Lactobacillus and Bifidobacterium species constitute a significant proportion of probiotic cultures used in developed countries in ‘microbial adjunct nutrition’. A number of differential plating methodologies have been developed which seek to selectively detect and enumerate these bacterial groups in bioproducts. Differences in oxygen tolerance, nutritional requirements, antibiotic susceptibility, and colony morphology and colour constitute the bases of differentiation in these

William P. Charteris; Phillip M. Kelly; Lorenzo Morelli; J. Kevin Collins

1997-01-01

300

Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures  

Microsoft Academic Search

The adhesion of 12 different Lactobacillus strains was studied using Caco-2 cell line as an in vitro model for intestinal epithelium. Some of the strains tested have been used as probiotics, and most of them are used in the dairy and food industry. Human and bovine enterotoxigenic Escherichia coli strains were used as positive and negative control, respectively. Bacterial adhesion

Seppo J Salminen

1998-01-01

301

Integrative Food-Grade Expression System Based on the Lactose Regulon of Lactobacillus casei  

Microsoft Academic Search

The lactose operon from Lactobacillus casei is regulated by very tight glucose repression and substrate induction mechanisms, which made it a tempting candidate system for the expression of foreign genes or metabolic engineering. An integrative vector was constructed, allowing stable gene insertion in the chromo- somal lactose operon of L. casei. This vector was based on the nonreplicative plasmid pRV300

M. J. Gosalbes; CARLOS DAVID ESTEBAN; JOSELUIS GALAN; GASPAR PEREZ-MARTINEZ

2000-01-01

302

Characterization of the tre Locus and Analysis of Trehalose Cryoprotection in Lactobacillus acidophilus NCFM  

Microsoft Academic Search

Freezing and lyophilization are common methods used for preservation and storage of microorganisms during the production of concentrated starter cultures destined for industrial fermentations or product formulations. The compatible solute trehalose has been widely reported to protect bacterial, yeast and animal cells against a variety of environmental stresses, particularly freezing and dehydration. Analysis of the Lactobacillus acidophilus NCFM genome revealed

Tri Duong; Rodolphe Barrangou; W. Michael Russell; Todd R. Klaenhammer

2006-01-01

303

Inhibitory activity spectrum of reuterin produced by Lactobacillus reuteri against intestinal bacteria  

Microsoft Academic Search

BACKGROUND: Reuterin produced from glycerol by Lactobacillus reuteri, a normal inhabitant of the human intestine, is a broad-spectrum antimicrobial agent. It has been postulated that reuterin could play a role in the probiotic effects of Lb. reuteri. Reuterin is active toward enteropathogens, yeasts, fungi, protozoa and viruses, but its effect on commensal intestinal bacteria is unknown. Moreover reuterin's mode of

Valentine Cleusix; Christophe Lacroix; Sabine Vollenweider; Marc Duboux; Gwenaelle Le Blay

2007-01-01

304

Engineered Vaginal Lactobacillus Strain for Mucosal Delivery of the Human Immunodeficiency Virus Inhibitor Cyanovirin-N  

Microsoft Academic Search

Women are at significant risk of human immunodeficiency virus (HIV) infection, with the cervicovaginal mucosa serving as a major portal for virus entry. Female-initiated preventatives, including topical microbi- cides, are urgently needed to help curtail the HIV\\/AIDS pandemic. Here we report on the development of a novel, live microbicide that employs a natural vaginal strain of Lactobacillus jensenii engineered to

Xiaowen Liu; Laurel A. Lagenaur; David A. Simpson; Kirsten P. Essenmacher; Courtney L. Frazier-Parker; Yang Liu; Daniel Tsai; Srinivas S. Rao; Dean H. Hamer; Thomas P. Parks; Peter P. Lee; Qiang Xu

2006-01-01

305

Identification and Inactivation of Genetic Loci Involved with Lactobacillus acidophilus Acid Tolerance  

Microsoft Academic Search

Amino acid decarboxylation-antiporter reactions are one of the most important systems for maintaining intracellular pH between physiological limits under acid stress. We analyzed the Lactobacillus acidophilus NCFM complete genome sequence and selected four open reading frames with similarities to genes involved with decarboxylation reactions involved in acid tolerance in several microorganisms. Putative genes encoding an ornithine decarboxylase, an amino acid

M. Andrea Azcarate-Peril; Eric Altermann; Rebecca L. Hoover-Fitzula; Raul J. Cano; Todd R. Klaenhammer

2004-01-01

306

Production of Single Cell Protein in Stickwater by Lactobacillus acidophilus and Aspergillus niger  

Microsoft Academic Search

The aim of this study was to investigate production of single cell protein (SCP) using Lactobacillus acidophilus and Aspergillus niger in stickwater from fish meal factories. Stickwater was used as substrate for L. acidophilus and A. niger and compared with standard medium as control. The maximum chemical oxygen demand reduction by L. acidophilus was achieved at 55.4 and 86.4% and

Safarbibi Kam; Abdolmohammad Abedian Kenari; Habibollah Younesi

2011-01-01

307

Spatial and temporal expression of Lactobacillus plantarum genes in the gastrointestinal tracts of mice  

Microsoft Academic Search

Lactobacillus plantarum is a common inhabitant of mammalian gastrointestinal tracts, and L. plantarum strain WCFS1 is a human isolate with a known genome sequence. L. plantarum WCFS1 survives intestinal passage in an active form, and its transit time and transcriptional activities were monitored in 15 BALB\\/c mice at 2, 4, 6, 8, and 24 h after being fed a single

Maria L. Marco; Roger S. Bongers; Vos de W. M; Michiel Kleerebezem

2007-01-01

308

Influence of Fermentation Medium Composition on Physicochemical Surface Properties of Lactobacillus acidophilus  

Microsoft Academic Search

The effect of the simple and complex basic components of a fermentation medium on the surface properties of Lactobacillus acidophilus NCC2628 is studied by physicochemical methods, such as electrophoresis, inter- facial adhesion, and X-ray photonelectron spectroscopy, and by transmission electron microscopy. Starting from an optimized complete medium, the effect of carbohydrates, peptones, and yeast extracts on the physi- cochemical properties

Prisca Schar-Zammaretti; Marie-Lise Dillmann; Nicola D'Amico; Michael Affolter; Job Ubbink

2005-01-01

309

Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates.  

PubMed

In this study, 15 Lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. The stationary-phase biosurfactants from lactobacillus casei subsp. rhamnosus 36 and ATCC 7469, Lactobacillus fermentum B54, and Lactobacillus acidophilus RC14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic Enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. The initial deposition rate of E. faecalis to glass with an adsorbed biosurfactant layer from L. acidophilus RC14 or L. fermentum B54 was significantly decreased by approximately 70%, while the number of adhering enterococci after 4 h of adhesion was reduced by an average of 77%. The surface activity of the biosurfactants and their activity inhibiting the initial adhesion of E. faecalis 1131 were retained after dialysis (molecular weight cutoff, 6,000 to 8,000) and freeze-drying. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy revealed that the freeze-dried biosurfactants from L. acidophilus RC14 and L. fermentum B54 were richest in protein, while those from L. casei subsp. rhamnosus 36 and ATCC 7469 had relatively high polysaccharide and phosphate contents. PMID:8787394

Velraeds, M M; van der Mei, H C; Reid, G; Busscher, H J

1996-06-01

310

Lactobacillus sanfrancisco a key sourdough lactic acid bacterium:a review  

Microsoft Academic Search

Sourdough may be considered a traditional product with a great future. Studies on the sourdough microflora have recently been improved. In this paper, research onLactobacillus sanfrancisco, a key sourdough lactic acid bacterium, is reviewed. The physiology, trophic relationships with sourdough related organisms and the genetics are considered in order to explain and improve the biotechnological performances ofLb. sanfrancisco.

M. Gobbetti; A. Corsetti

1997-01-01

311

Inhibition of Listeria monocytogenes in a pork product by a Lactobacillus sake strain  

Microsoft Academic Search

A Lactobacillus sake strain (L. sake 2a), isolated from Brazilian sausage (`lingüiça'), possessed antilisterial activity. The inhibitory activity observed in culture media was due to a proteinaceous compound, as demonstrated by the sensitivity to proteolytic enzymes. Inhibition due to hydrogen peroxide, organic acids and lytic bacteriophages was ruled out. The inhibition of Listeria monocytogenes by L. sake 2a was also

Elaine C. P De Martinis; Bernadette D. G. M Franco

1998-01-01

312

Genome Sequence Analysis of the Biogenic Amine-Degrading Strain Lactobacillus casei 5b.  

PubMed

We here report a 3.02-Mbp annotated draft assembly of the Lactobacillus casei 5b genome. The sequence of this biogenic amine-degrading dairy isolate may help identify the mechanisms involved in the catabolism of biogenic amines and perhaps shed light on ways to reduce the presence of these toxic compounds in food. PMID:24435875

Ladero, Victor; Herrero-Fresno, Ana; Martinez, Noelia; Del Río, Beatriz; Linares, Daniel M; Fernández, María; Martín, María Cruz; Alvarez, Miguel A

2014-01-01

313

Genome Sequence Analysis of the Biogenic Amine-Degrading Strain Lactobacillus casei 5b  

PubMed Central

We here report a 3.02-Mbp annotated draft assembly of the Lactobacillus casei 5b genome. The sequence of this biogenic amine-degrading dairy isolate may help identify the mechanisms involved in the catabolism of biogenic amines and perhaps shed light on ways to reduce the presence of these toxic compounds in food.

Ladero, Victor; Herrero-Fresno, Ana; Martinez, Noelia; del Rio, Beatriz; Linares, Daniel M.; Fernandez, Maria; Martin, Maria Cruz

2014-01-01

314

Accumulation of polyphosphate in Lactobacillus spp. and its involvement in stress resistance.  

PubMed

Polyphosphate (poly-P) is a polymer of phosphate residues synthesized and in some cases accumulated by microorganisms, where it plays crucial physiological roles such as the participation in the response to nutritional stringencies and environmental stresses. Poly-P metabolism has received little attention in Lactobacillus, a genus of lactic acid bacteria of relevance for food production and health of humans and animals. We show that among 34 strains of Lactobacillus, 18 of them accumulated intracellular poly-P granules, as revealed by specific staining and electron microscopy. Poly-P accumulation was generally dependent on the presence of elevated phosphate concentrations in the culture medium, and it correlated with the presence of polyphosphate kinase (ppk) genes in the genomes. The ppk gene from Lactobacillus displayed a genetic arrangement in which it was flanked by two genes encoding exopolyphosphatases of the Ppx-GppA family. The ppk functionality was corroborated by its disruption (LCABL_27820 gene) in Lactobacillus casei BL23 strain. The constructed ppk mutant showed a lack of intracellular poly-P granules and a drastic reduction in poly-P synthesis. Resistance to several stresses was tested in the ppk-disrupted strain, showing that it presented a diminished growth under high-salt or low-pH conditions and an increased sensitivity to oxidative stress. These results show that poly-P accumulation is a characteristic of some strains of lactobacilli and may thus play important roles in the physiology of these microorganisms. PMID:24375133

Alcántara, Cristina; Blasco, Amalia; Zúñiga, Manuel; Monedero, Vicente

2014-03-01

315

Genome Sequence and Characteristics of Lrm1, a Prophage from Industrial Lactobacillus rhamnosus Strain M1  

Microsoft Academic Search

Prophage Lrm1 was induced with mitomycin C from an industrial Lactobacillus rhamnosus starter culture, M1. Electron microscopy of the lysate revealed relatively few intact bacteriophage particles among empty heads and disassociated tails. The defective Siphoviridae phage had an isometric head of approximately 55 nm and noncontractile tail of about 275 nm with a small baseplate. In repeated attempts, the prophage

Evelyn Durmaz; Michael J. Miller; M. Andrea Azcarate-Peril; Stephen P. Toon; Todd R. Klaenhammer

2008-01-01

316

Antibiotic Susceptibility of Lactobacillus and Bifidobacterium Species from the Human Gastrointestinal Tract  

Microsoft Academic Search

One hundred and twenty-two strains of Bifidobacterium and Lactobacillus species have been tested against 12 antibiotics and two antibiotic mixtures by a commercial system (Sensititre Anaero3; Treck Diagnostic Systems). The upper limits of some minimum inhibitory concentrations (MICs) were completed on MRS agar plates by the NCCLS procedure. All strains were sensitive to chloramphenicol and imipenem and most of the

Susana Delgado; Ana Belén Flórez; Baltasar Mayo

2005-01-01

317

Genome Sequence of the Vertebrate Gut Symbiont Lactobacillus reuteri ATCC 53608 ?  

PubMed Central

Lactobacillus reuteri, inhabiting the gastrointestinal tracts of a range of vertebrates, is a true symbiont with effects established as beneficial to the host. Here we describe the draft genome of L. reuteri ATCC 53608, isolated from a pig. The genome sequence provides important insights into the evolutionary changes underlying host specialization.

Heavens, Darren; Tailford, Louise E.; Crossman, Lisa; Jeffers, Faye; MacKenzie, Donald A.; Caccamo, Mario; Juge, Nathalie

2011-01-01

318

Characterization of a Lactobacillus gasseri JCM 1131T Lipoteichoic Acid with a Novel Glycolipid Anchor Structure  

PubMed Central

We determined the chemical structure of lipoteichoic acid (LTA) from Lactobacillus gasseri JCM 1131T. The repeating unit was comprised of glycerolphosphate and 2-alanylglycerolphosphate. The glycolipid anchor was tetrahexosylglycerol with two or three acyl groups. To our knowledge, this is the first demonstration of a tetrahexose structure in an LTA glycolipid anchor.

Shiraishi, Tsukasa; Yokota, Shin-ichi; Morita, Naoki; Fukiya, Satoru; Tomita, Satoru; Tanaka, Naoto; Okada, Sanae

2013-01-01

319

Degradation of ascorbic acid and potassium sorbate by different Lactobacillus species isolated from packed green olives.  

PubMed

The aim of this research was to ascertain the lactic acid bacteria responsible for the degradation of ascorbic acid and/or potassium sorbate, isolated from packed green olives where these additives had diminished. A total of 14 isolates were recovered from samples of different green olive containers. According to partial sequencing of the 16S rRNA coding gene, Lactobacillus parafarraginis, Lactobacillus rapi, Lactobacillus pentosus, Lactobacillus paracollinoides, and Pediococcus ethanolidurans were identified. With the exception of L. pentosus and L. paracollinoides, the other species had not been mentioned in table olives before this study. Only three of the 14 isolates metabolized ascorbic acid in MRS broth, and the products from ascorbic acid in modified MRS broth without carbon sources were acetic and lactic acids. Except for the two L. rapi and the two P. ethanolidurans strains, the remaining 10 isolates depleted potassium sorbate added into MRS broth to some extent. The product generated by three of these strains was confirmed to be trans-4-hexenoic acid. The degradation of ascorbate or sorbate by lactic acid bacteria should be taken into account when these additives are used in food products where this group of bacteria may be present. PMID:23498172

Montaño, Alfredo; Sánchez, Antonio Higinio; Casado, Francisco Javier; Beato, Víctor Manuel; de Castro, Antonio

2013-05-01

320

Bactericidal action of oleuropein extracted from green olives against Lactobacillus plantarum  

Microsoft Academic Search

The phenolic compound oleuropein extracted from green olives was shown to be bactericidal against nine strains of Lactobacillus plantarum isolated from green olive fermentation brines. Heat-treated oleuropein also demonstrated a strong bacteri- cidal effect but not alkali-treated oleuropein, which allowed survival of most of the strains tested. The bactericidal effect was accompanied by changes in the typical bacillary structure and

J. L. Ruiz-Barba; A. Garrido-Fernandez; R. Jimenez-Diaz

1991-01-01

321

Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.  

PubMed

The microbiota of the rat intestinal tract constitutes a complex ecosystem of microorganisms. We have developed a real-time quantitative PCR assay based on genus-specific 16S rDNA primers and 3' minor groove binder (MGB) probes for accurate detection and quantification of a wide range of Bifidobacterium spp. (30 species) and Lactobocillus spp. (15 species) in rat fecal samples. Real-time PCR detection of serially diluted DNA isolated from reference strains of Bifidobacterium longum and Lactobacillus acidophilus was linear for cell counts ranging from 10(6) to 10 cells per PCR assay. The method proved applicable to the detection of Bifidobacterium spp. and Lactobacillus spp. at concentrations down to 10 CFU per PCR, corresponding to 5 x 10(4) CFU/g feces. The inter-extract reproducibility was high, with a coefficient of variation ranging from 0.24% to 1.07% for the Bifidobacterium assay and from 0.05% to 1.28% for the Lactobacillus assay. We conclude that real-time PCR is a very sensitive and precise technique for extensive quantitative evaluation of gut Bifidobacterium spp. and Lactobacillus spp. Thus, the approach used here to detect and quantify bacteria with group-specific primers should contribute to further studies of the composition and dynamics of the rat intestinal microbiota. PMID:19216105

Delroisse, Jean-Marc; Boulvin, Anne-Lise; Parmentier, Isabelle; Dauphin, Robin Dubois; Vandenbol, Micheline; Portetelle, Daniel

2008-01-01

322

Protein-mediated Adhesion of Lactobacillus fermentum Strain 737 to Mouse Stomach Squamous Epithelium  

Microsoft Academic Search

The mechanism of adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium was investigated. Adhesion inhibition tests involving chelators, monosaccharides, periodate and concanavalin A and the use of bacteria grown in the presence of tunicamycin failed to clarify the adhesive mechanism. Washed bacterial cells had reduced adhesive capacity, except in the presence of spent broth culture supernatant fraction

PATRICIA L. CONWAY; STAFFAN KJELLEBERG

1989-01-01

323

Complete Genome Sequence of the Probiotic Bacterium Lactobacillus casei LC2W?  

PubMed Central

Lactobacillus casei LC2W, a patented probiotic strain (Z. Wu, European patent EP 1642963 B1, February 2009), has been isolated from Chinese traditional dairy products and implemented in industrial production as starter culture. Here we present the complete genome sequence of LC2W and the identification of a gene cluster implicated in the biosynthesis of exopolysaccharides.

Chen, Chen; Ai, Lianzhong; Zhou, Fangfang; Wang, Lei; Zhang, Hao; Chen, Wei; Guo, Benheng

2011-01-01

324

Complete Genome Sequence of the Probiotic Strain Lactobacillus casei BD-II ?  

PubMed Central

Lactobacillus casei BD-II, a patented probiotic strain (U.S. patent 7,270,994 B2), was isolated from homemade koumiss in China and has been implemented in the industrial production as starter cultures. Here we report the complete genome sequence of BD-II, which shows high similarity with the well-studied probiotic BL23.

Ai, Lianzhong; Chen, Chen; Zhou, Fangfang; Wang, Lei; Zhang, Hao; Chen, Wei; Guo, Benheng

2011-01-01

325

Genome Sequence of the Cheese-Starter Strain Lactobacillus delbrueckii subsp. lactis CRL 581  

PubMed Central

We report the genome sequence of Lactobacillus delbrueckii subsp. lactis CRL 581 (1,911,137 bp, GC 49.7%), a proteolytic strain isolated from a homemade Argentinian hard cheese which has a key role in bacterial nutrition and releases bioactive health-beneficial peptides from milk proteins.

Raya, Raul R.; Brown, Lucia; Font de Valdez, Graciela; Savoy de Giori, Graciela; Taranto, Maria Pia

2013-01-01

326

Complete genome sequence of Lactobacillus reuteri I5007, a probiotic strain isolated from healthy piglet.  

PubMed

Lactobacillus reuteri I5007 is a well-characterized probiotic strain isolated from the colonic mucosa of healthy weaning piglets. Here, we present the complete genome sequence of this strain, which consists of a circular chromosome and six distinct plasmids. PMID:24685642

Hou, Chengli; Wang, Qingwei; Zeng, Xiangfang; Yang, Fengjuan; Zhang, Jiang; Liu, Hong; Ma, Xi; Qiao, Shiyan

2014-06-10

327

A rapid procedure for isolating chromosomal DNA from Lactobacillus species and other Gram-positive bacteria  

Microsoft Academic Search

R. L. U L R I C H A N D T. A. H U G H E S. 2001. This study describes a rapid procedure for the isolation of genomic DNA from various Gram-positive bacteria. Species tested included Lactobacillus delbrueckii subsp. lactis ATCC 4797, Lact. acidophilus N2, Staphylococcus aureus, Staph. epidermidis, Propionibacterium jensenii P126, Bacillus pumilus and Enterococcus faecalis.

R. L. Ulrich; T. A. Hughes

2001-01-01

328

Complete Genome Sequence for Lactobacillus helveticus CNRZ 32, an Industrial Cheese Starter and Cheese Flavor Adjunct  

PubMed Central

Lactobacillus helveticus is a lactic acid bacterium widely used in the manufacture of cheese and for production of bioactive peptides from milk proteins. We present the complete genome sequence for L. helveticus CNRZ 32, a strain particularly recognized for its ability to reduce bitterness and accelerate flavor development in cheese.

Hughes, Joanne E.; Welker, Dennis L.; Tompkins, Thomas A.; Steele, James L.

2013-01-01

329

Draft Genome Sequence of Lactobacillus plantarum wikim18, Isolated from Korean Kimchi.  

PubMed

This report describes the draft genome sequence of Lactobacillus plantarum strain wikim18, isolated from the traditional Korean food kimchi. The reads generated by Ion Torrent PGM were assembled into 327 contigs. RAST annotation of the genome revealed 12 tRNAs and 3,316 protein-coding gene sequences. PMID:24855305

Jang, Ja Young; Lim, Hyeong In; Park, Hae Woong; Choi, Hak-Jong; Kim, Tae-Woon; Kang, Miran; Lee, Jong-Hee

2014-01-01

330

Genome sequence of Lactobacillus ingluviei, a bacterium associated with weight gain in animals.  

PubMed

We report the draft genome sequence of Lactobacillus ingluviei strain Autruche 4 (CSURP209) isolated from an ostrich. L. ingluviei is associated with weight gain in mice. This genome sequence may help us understand the obesity-induced mechanisms of intestinal bacteria. PMID:23012279

Merhej, Vicky; Armougom, Fabrice; Robert, Catherine; Raoult, Didier

2012-10-01

331

Strain-dependent induction of cytokine profiles in the gut by orally administered Lactobacillus strains  

Microsoft Academic Search

Different Lactobacillus strains are frequently used in consumer food products. In addition, recombinant lactobacilli which contain novel expression vectors can now be used in immunotherapeutic applications such as oral vaccination strategies and in T cell tolerance induction approaches for autoimmune disease. Both for food and clinical applications of lactobacilli, proper selection of wild type strains is crucial.For that purpose, eight

Catharina B. M. Maassen; Conny van Holten-Neelen; Fräncis Balk; Marie-Joan Heijne den Bak-Glashouwer; Rob J. Leer; Jon D. Laman; Wim J. A. Boersma; Eric Claassen

2000-01-01

332

Alterations in fecal Lactobacillus and Bifidobacterium species in type 2 diabetic patients in Southern China population  

PubMed Central

Background: The connection between gut microbiota and metabolism and its role in the pathogenesis of diabetes are increasingly recognized. The objective of this study was to quantitatively measure Bifidobacterium and Lactobacillus species, members of commensal bacteria found in human gut, in type 2 diabetic patients (T2D) patients from Southern China. Methods: Fifty patients with T2D and thirty control individuals of similar body mass index (BMI) were recruited from Southern China. T2D and control subjects were confirmed with both oral glucose tolerance test (OGTT) and HbA1c measurements. Bifidobacterium and Lactobacillus species in feces were measured by real-time quantitative PCR. Data were analyzed with STATA 11.0 statistical software. Results: In comparison to control subjects T2D patients had significantly more total Lactobacillus (+18%), L. bugaricus (+13%), L. rhamnosum (+37%) and L. acidophilus (+48%) (P < 0.05). In contrast, T2D patients had less amounts of total Bifidobacteria (?7%) and B. adolescentis (?12%) (P < 0.05). Cluster analysis showed that gut microbiota pattern of T2D patients is characterized by greater numbers of L. rhamnosus and L. acidophillus, together with lesser numbers of B. adolescentis (P < 0.05). Conclusion: The gut microflora in T2D patients is characterized by greater numbers of Lactobacillus and lesser numbers of Bifidobacterium species.

Le, Kim-Anne; Li, Yan; Xu, Xiaojing; Yang, Wanting; Liu, Tingting; Zhao, Xiaoning; Tang, Yongming Gorge; Cai, Dehong; Go, Vay Liang W.; Pandol, Stephen; Hui, Hongxiang

2013-01-01

333

Purification and amino acid sequence of lactocin 705, a bacteriocin produced by Lactobacillus casei CRL 705  

Microsoft Academic Search

Lactobacillus casei CRL 705, isolated from a dry fermented sausage, produces an antibacterial peptide which is active against Listeria monocytogenes. Previous studies have shown that this compound is potentially useful to control food-borne pathogens in ground meat. In view of the potential application of this antimicrobial substance in food fermentation, a detailed biochemical analysis of this peptide is required. In

J. Palacios; G. Vignolo; M. E. FarÍas; A. P. de Ruiz Holgado; G. Oliver; F. Sesma

1999-01-01

334

Characterization of Muscle Sarcoplasmic and Myofibrillar Protein Hydrolysis Caused by Lactobacillus plantarum  

Microsoft Academic Search

Strains of Lactobacillus plantarum originally isolated from sausages were screened for proteinase and aminopeptidase activities toward synthetic substrates; on the basis of that screening, L. plantarum CRL 681 was selected for further assays on muscle proteins. The activities of whole cells, cell extracts (CE), and a combination of both on sarcoplasmic and myofibrillar protein extracts were determined by protein, peptide,

SILVINA FADDA; YOLANDA SANZ; GRACIELA VIGNOLO; GUILLERMO OLIVER; FIDEL TOLDRA ´

1999-01-01

335

Effect of curing conditions and Lactobacillus casei CRL705 on the hydrolysis of meat proteins  

Microsoft Academic Search

S. F ADDA, G. V IGNOLO, M.-C. A RISTOY, G. O LIVER A ND F. T OLDRA ´ . 2001. Aims: The effect of the common curing conditions used during the manufacture of dry fermented sausage on the proteolytic activity of Lactobacillus casei CRL705 against meat proteins was investigated. Methods and Results: Hydrolysis of pork muscle sarcoplasmic and myofibrillar proteins

S. Fadda; G. Vignolo; M.-C. Aristoy; G. Oliver; F. Toldra

2001-01-01

336

Draft Genome Sequence of Lactobacillus plantarum wikim18, Isolated from Korean Kimchi  

PubMed Central

This report describes the draft genome sequence of Lactobacillus plantarum strain wikim18, isolated from the traditional Korean food kimchi. The reads generated by Ion Torrent PGM were assembled into 327 contigs. RAST annotation of the genome revealed 12 tRNAs and 3,316 protein-coding gene sequences.

Jang, Ja Young; Lim, Hyeong In; Park, Hae Woong; Choi, Hak-Jong; Kim, Tae-Woon; Kang, Miran

2014-01-01

337

Optimization of media and fermentation conditions for the growth of Lactobacillus sakei  

Microsoft Academic Search

Factorial experiments, empirical model building and response surface analysis were used to optimize media composition and fermentation conditions for the propagation of Lactobacillus sakei, a species used as a starter in the manufacture of fermented sausages. Yeast extract, Lab-Lemco and, to a minor extent, Bacteriological peptone were found to be good sources of peptides and growth factors for L. sakei,

T. LECHIANCOLE; A. RICCIARDI; E. PARENTE

338

Enterococcus and Lactobacillus contamination of raw milk in a farm dairy environment.  

PubMed

Enterococci and lactobacilli are ubiquitously found in the intestinal microflora of humans and animals. The aim of the present study was to determine the importance of bovine faeces as a source of these organisms in raw milk. One hundred and fifty six putative enterococci and 362 lactobacilli were isolated from bovine faeces (n=26), cows' teats, raw milk, the milking machine and the milking environment on one farm. The clonal relationships of each group were investigated using Pulsed-Field Gel Electrophoresis and representatives of the different clusters were identified by repetitive DNA element (rep)-PCR fingerprinting, protein profiling, phenylalanyl-tRNA synthase (pheS) sequence analysis or 16S rDNA gene sequencing. Lactobacilli were present at approximately 3 orders of magnitude greater than enterococci in the bovine faeces. The majority of the bovine faecal enterococcal isolates were identified as Aerococcus viridans. Seven teat isolates belonged to a potential novel Aerococcus sp. and one bovine faecal isolate to a potential second novel Aerococcus sp. The lactobacilli present in the bovine faeces were predominantly Lactobacillus mucosae and Lactobacillus brevis, with small numbers of Lactobacillus plantarum. Only one Enterococcus (a strain of E. casseliflavus) out of 76 and one Lactobacillus (a strain of L. parabuchneri/kefir) out of 247 of the bovine faecal isolates was found in the milk. The major source of these bacteria in the milk was the milking equipment. PMID:17189657

Kagkli, Dafni Maria; Vancanneyt, Marc; Hill, Colin; Vandamme, Peter; Cogan, Timothy M

2007-03-10

339

Genome-shuffling improved acid tolerance and l-lactic acid volumetric productivity in Lactobacillus rhamnosus  

Microsoft Academic Search

Genome shuffling is an efficient approach for the rapid improvement of industrially important microbial phenotypes. Here we improved the acid tolerance and volumetric productivity of an industrial strain Lactobacillus rhamnosus ATCC 11443 by genome shuffling. Five strains with subtle improvements in pH tolerance and volumetric productivity were obtained from the populations generated by ultraviolet irradiation and nitrosoguanidine mutagenesis, and then

Yuhua Wang; Yan Li; Xiaolin Pei; Lei Yu; Yan Feng

2007-01-01

340

Genome shuffling enhanced l-lactic acid production by improving glucose tolerance of Lactobacillus rhamnosus  

Microsoft Academic Search

Genome shuffling is a powerful strategy for rapid engineering of microbial strains for desirable industrial phenotypes. Here we applied the genome shuffling to improve the glucose tolerance of Lactobacillus rhamnosus ATCC 11443 while simultaneously enhancing the l-lactic acid production. The starting population was generated by ultraviolet irradiation and nitrosoguanidine mutagenesis and then subjected for the recursive protoplast fusion. The positive

Lei Yu; Xiaolin Pei; Ting Lei; Yuhua Wang; Yan Feng

2008-01-01

341

Characterization and site-directed mutagenesis of Wzb, an O-phosphatase from Lactobacillus rhamnosus  

Microsoft Academic Search

BACKGROUND: Reversible phosphorylation events within a polymerisation complex have been proposed to modulate capsular polysaccharide synthesis in Streptococcus pneumoniae. Similar phosphatase and kinase genes are present in the exopolysaccharide (EPS) biosynthesis loci of numerous lactic acid bacteria genomes. RESULTS: The protein sequence deduced from the wzb gene in Lactobacillus rhamnosus ATCC 9595 reveals four motifs of the polymerase and histidinol

Gisèle LaPointe; Danièle Atlan; Christophe Gilbert

2008-01-01

342

Optimization of lactic acid production from beet molasses by Lactobacillus delbrueckii NCIMB 8130  

Microsoft Academic Search

Production of lactic acid from beet molasses by Lactobacillus delbrueckii NCIMB 8130 in static and shake flask fermentation was investigated. Shake flasks proved to be a better fermentation system for this purpose. Substitution of yeast extract with other low cost protein sources did not improve lactic acid production. The maximum lactic acid concentration was achieved without treatment of molasses. A

Ch. Kotzamanidis; T. Roukas; G. Skaracis

2002-01-01

343

Phenotypic and genomic diversity of Lactobacillus plantarum strains isolated from various environmental niches  

Microsoft Academic Search

Lactobacillus plantarum is a ubiquitous microorganism that is able to colonize several ecological niches, including vegetables, meat, dairy substrates and the gastro-intestinal tract. An extensive phenotypic and genomic diversity analysis was conducted to elucidate the molecular basis of the high flexibility and versatility of this species. First, 185 isolates from diverse environments were phenotypically characterized by evaluating their fermentation and

Roland J. Siezen; Vesela A. Tzeneva; Anna Castioni; Michiel Wels; Hoa T. K. Phan; Jan L. W. Rademaker; Marjo J. C. Starrenburg; Michiel Kleerebezem; Douwe Molenaar; Hylckama Vlieg van J. E. T

2010-01-01

344

Development of microparticulate systems for intestinal delivery of Lactobacillus acidophilus and Bifidobacterium lactis  

Microsoft Academic Search

In the present study intestinal delivery systems resistant to gastric juice, loaded with the probiotic bacteria Lactobacillus acidophilus LA14 and Bifidobacterium lactis BI07, were produced by the polyelectrolyte complexation. First, beads were prepared by the traditional extrusion method and nine formulations were developed using alginate as main carrier and the biopolymer, xanthan gum (XG), as hydrophilic retardant polymer or the

Beatrice Albertini; Beatrice Vitali; Nadia Passerini; Federica Cruciani; Marcello Di Sabatino; Lorenzo Rodriguez; Patrizia Brigidi

2010-01-01

345

Analysis of exopolysaccharide production by Lactobacillus casei CG11, isolated from cheese.  

PubMed Central

Exopolysaccharide-producing Lactobacillus casei CG11 was isolated from soft, white, homemade cheese. In basal minimal medium, it produces a neutral heteropolysaccharide consisting predominantly of glucose (about 75%) and rhamnose (about 15%). Plasmid curing experiments revealed that exopolysaccharide production by strain CG11 is linked to a plasmid approximately 30 kb in size. Images

Kojic, M; Vujcic, M; Banina, A; Cocconcelli, P; Cerning, J; Topisirovic, L

1992-01-01

346

Development of a growth medium suitable for exopolysaccharide production by Lactobacillus delbrueckii ssp. bulgaricus RR  

Microsoft Academic Search

Complex media are commonly used in studies examining exopolysaccharide production by Lactobacillus delbrueckii ssp. bulgaricus. However, quantification of exopolysaccharide in complex medium can be complicated by interference due to carbohydrate polymers contained in media components. This study was undertaken to identify components of MRS, a common medium for cultivation of L. delbrueckii ssp. bulgaricus, that interfere with exopolysaccharide quantification, to

Stacy A Kimmel; Robert F Roberts

1998-01-01

347

Oral Immunization with Recombinant Lactobacillus plantarum Induces a Protective Immune Response in Mice with Lyme Disease  

Microsoft Academic Search

Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as Lactobacillus spp. In this study, we used the Lyme disease mouse model as a proof of concept. We demonstrate that an oral

Beatriz del Rio; Raymond J. Dattwyler; Miguel Aroso; Vera Neves; Luciana Meirelles; Jos F. M. L. Seegers; Maria Gomes-Solecki

2008-01-01

348

Antihypertensive activity of casein-enriched milk fermented by Lactobacillus helveticus  

Microsoft Academic Search

Milk proteins contain peptidic angiotensin I-converting enzyme (ACE) inhibitors, which can be released by proteolysis during milk fermentation by some strains of Lactobacillus helveticus. Reconstituted milk media containing skim milk powder (12%), skim milk powder (10%) with added sodium caseinate (5%) or whey protein isolate (5%) were fermented by L. helveticus strains R211 and R389, and further tested for bacterial

Pierre-Louis Leclerc; Sylvie F. Gauthier; Hélène Bachelard; Marta Santure; Denis Roy

2002-01-01

349

Improved Draft Genome Sequence of Probiotic Strain Lactobacillus gasseri K7  

PubMed Central

Lactobacillus gasseri K7 is an isolate from infant feces and has in vitro and in vivo established probiotic properties. Here, we report the improved version of the draft genome sequence, which comprises 8 scaffolds (13 contigs), a total length of 1.99 Mb, and 1,841 predicted protein-coding sequences.

Trmcic, Aljosa; Bogovic Matijasic, Bojana; Rogelj, Irena

2014-01-01

350

Bebek Mamalari ve Çocuk Ek Besinlerinde Lactobacillus rhamnosus GG Kullaniminin Sa?lik Üzerine Etkileri  

Microsoft Academic Search

Probiotics can be defined as living microorganisms of which has proved beneficial effects on health of the host and that modulate the intestinal flora. Lactobacillus rhamnosus GG is one of the widely used probiotic. In recent years the organism is frequently used in infants formulas and children's food due to preventive and curing effects on diarrho ea, dental caries, allergy

Zeynep Canbulat; Tülay Özcan

351

Protective action of Lactobacillus kefir carrying Slayer protein against Salmonella enterica serovar Enteritidis  

Microsoft Academic Search

Eight Lactobacillus kefir strains isolated from different kefir grains were tested for their ability to antagonize Salmonella enterica serovar Enteritidis (Salmonella enteritidis) interaction with epithelial cells. L. kefir surface properties such as autoaggregation and coaggregation with Salmonella and adhesion to Caco-2\\/TC-7 cells were evaluated. L. kefir strains showed significantly different adhesion capacities, six strains were able to autoaggregate and four

M. A. Golowczyc; P. Mobili; G. L. Garrote; A. G. Abraham; G. L. De Antoni

2007-01-01

352

Reduction of acetophenone to R (+)-phenylethanol by a new alcohol dehydrogenase from Lactobacillus kefir  

Microsoft Academic Search

A new alcohol dehydrogenase catalysing the enantioselective reduction of acetophenone to R(+)-phenylethanol was found in a strain of Lactobacillus kefir. A 70-fold enrichment of the enzyme with an overall yield of 76% was obtained in two steps. The addition of Mg2+ ions was found to be necessary to prevent rapid deactivation. The enzyme depends essentially on NADPH and was inactive

Werner Hummel

1990-01-01

353

Production of lactic acid by Lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate  

Microsoft Academic Search

Batch fermentation studies were performed to evaluate the potentials of a complex nitrogen source, soybean, as an alternative to yeast extract for the economical production of lactic acid by Lactobacillus rhamnosus. An enzyme-hydrolysate of soybean meal, Soytone, with an adequate supplementation of vitamins was found to be highly effective in supporting lactic acid production from glucose and lactose. The effects

Sunhoon Kwon; Pyung Cheon Lee; Eun Gyo Lee; Yong Keun Chang; Nam Chang

2000-01-01

354

Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus  

PubMed Central

Objectives Recurrent caries was partly ascribed to lack of antibacterial properties in composite resin. Silver and zinc nanoparticles are considered to be broad-spectrum antibacterial agents. The aim of the present study was to evaluate the antibacterial properties of composite resins containing 1% silver and zinc-oxide nanoparticles on Streptococcus mutans and Lactobacillus. Materials and Methods Ninety discoid tablets containing 0%, 1% nano-silver and 1% nano zinc-oxide particles were prepared from flowable composite resin (n = 30). The antibacterial properties of composite resin discs were evaluated by direct contact test. Diluted solutions of Streptococcus mutans (PTCC 1683) and Lactobacillus (PTCC 1643) were prepared. 0.01 mL of each bacterial species was separately placed on the discs. The discs were transferred to liquid culture media and were incubated at 37? for 8 hr. 0.01 mL of each solution was cultured on blood agar and the colonies were counted. Data was analyzed with Kruskall-Wallis and Mann-Whitney U tests. Results Composites containing nano zinc-oxide particles or silver nanoparticles exhibited higher antibacterial activity against Streptococcus mutans and Lactobacillus compared to the control group (p < 0.05). The effect of zinc-oxide on Streptococcus mutans was significantly higher than that of silver (p < 0.05). There were no significant differences in the antibacterial activity against Lactobacillus between composites containing silver nanoparticles and those containing zinc-oxide nanoparticles. Conclusions Composite resins containing silver or zinc-oxide nanoparticles exhibited antibacterial activity against Streptococcus mutans and Lactobacillus.

Kasraei, Shahin; Sami, Lida; Hendi, Sareh; AliKhani, Mohammad-Yousef; Rezaei-Soufi, Loghman

2014-01-01

355

Lactobacillus GG for treatment of acute childhood diarrhoea: An open labelled, randomized controlled trial  

PubMed Central

Background & objectives: Randomized controlled trials in developed countries have reported benefits of Lactobacillus GG (LGG) in the treatment of acute watery diarrhoea, but there is paucity of such data from India. The study was aimed to evaluate the efficacy and safety of Lactobacillus GG in the treatment of acute diarrhoea in children from a semi-urban city in north India. Methods: In this open labelled, randomized controlled trial 200 children with acute watery diarrhoea, aged between 6 months to 5 years visiting outpatient department and emergency room of a teaching hospital in north India were enrolled. The children were randomized into receiving either Lactobacillus GG in dose of 10 billion cfu/day for five days or no probiotic medication in addition to standard WHO management of diarrhoea. Primary outcomes were duration of diarrhoea and time to change in consistency of stools. Results: Median (inter quartile range) duration of diarrhoea was significantly shorter in children in LGG group [60 (54-72) h vs. 78 (72-90) h; P<0.001]. Also, there was faster improvement in stool consistency in children receiving Lactobacillus GG than control group [36 (30-36) h vs. 42 (36-48) h; P<0.001]. There was significant reduction in average number of stools per day in LGG group (P<0.001) compared to the control group. These benefits were seen irrespective of rotavirus positivity in stool tests. Interpretation & conclusions: Our results showed that the use of Lactobacillus GG in children with acute diarrhoea resulted in shorter duration and faster improvement in stool consistency as compared to the control group.

Aggarwal, Sunny; Upadhyay, Amit; Shah, Dheeraj; Teotia, Neeraj; Agarwal, Astha; Jaiswal, Vijay

2014-01-01

356

Consistent Condom Use Increases the Colonization of Lactobacillus crispatus in the Vagina  

PubMed Central

Background Non-hormonal contraception methods have been widely used, but their effects on colonization by vaginal lactobacilli remain unclear. Objective To determine the association between non-hormonal contraception methods and vaginal lactobacilli on women’s reproductive health. Methods The cross-sectional study included 164 healthy women between 18–45 years of age. The subjects were divided into different groups on the basis of the different non-hormonal contraception methods used by them. At the postmenstrual visit (day 21 or 22 of the menstrual cycle), vaginal swabs were collected for determination of Nugent score, quantitative culture and real-time polymerase chain reaction (PCR) of vaginal lactobacilli. The prevalence, colony counts and 16S rRNA gene expression of the Lactobacillus strains were compared between the different groups by Chi-square and ANOVA statistical analysis methods. Results A Nugent score of 0–3 was more common in the condom group (93.1%) than in the group that used an interuterine device(IUD) (75.4%), (p?=?0.005). The prevalence of H2O2-producing Lactobacillus was significantly higher in the condom group (82.3%) than in the IUD group (68.2%), (p?=?0.016). There was a significant difference in colony count (mean ± standard error (SE), log10colony forming unit (CFU)/ml) of H2O2-producing Lactobacillus between condom users (7.81±0.14) and IUD users (6.54±0.14), (p?=?0.000). The 16S rRNA gene expression (mean ± SE, log10copies/ml) of Lactobacillus crispatus was significantly higher in the condom group (8.09±0.16) than in the IUD group (6.03±0.18), (p?=?0.000). Conclusion Consistent condom use increases the colonization of Lactobacillus crispatus in the vagina and may protect against both bacterial vaginosis (BV) and human immunodeficiency virus (HIV).

Ma, Liyan; Lv, Zhi; Su, Jianrong; Wang, Jianjie; Yan, Donghui; Wei, Jingjuan; Pei, Shuang

2013-01-01

357

Vanadium and Experimental Caries. VII. Action of Vanadium on the Development of Lactobacillus Acidophilus and Streptococcus Lactis (Vanadio E Carie Sperimentale. VII. Azione del Vanadio sullo Sviluppo del Lactobacillus Acidophilus e dello Streptococcus Lactis).  

National Technical Information Service (NTIS)

The action of solutions with different contents of vanadium on the development and on the production of lactic acid of cultures of lactobacillus acidophilus and streptococcus lactis was investigated by the authors. The authors have established that there ...

G. Santacatterina G. Grippaudo F. Valfre G. Cecchetti

1972-01-01

358

Zymogram and Preliminary Characterization of Lactobacillus helveticus Autolysins  

PubMed Central

The autolysins of Lactobacillus helveticus ISLC5 were detected and partially characterized by renaturing sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis with substrate-containing gels (zymogram). By using lyophilized Micrococcus luteus cells or heated whole cells of L. helveticus ISLC5 (0.2% [wt/vol]) as a substrate, several lytic activities were detected in the whole-cell SDS extract of strain ISLC5 (i) one activity at 42.4 kDa, which was named autolysin A, and (ii) six other activities having very similar molecular weights (29.1, 29.6, 30, 30.8, 31.7, and 32.8 kDa), which were named autolysins B (B1 through B6, respectively). As regards the temporal distribution of the enzymes, autolysins A and B were detected in the cells harvested from the beginning of the exponential growth phase. Autolysin A appeared to be associated only with viable cells, whereas the autolysins B remained associated with the cell envelope several days after the complete loss of culture viability. When SDS-treated walls of L. helveticus ISLC5 were used as a substrate, a supplementary lytic activity appeared at 37.5 kDa; it was considered a peptidoglycan hydrolase, since it was not able to induce lysis of whole-cell substrate. The autolysins of 30 other strains of L. helveticus from various geographical origins were also analyzed by zymogram; all the activity profiles obtained were similar to that of strain ISLC5 in terms of the number of lytic bands and their apparent molecular weights. Only the relative intensities of the lytic bands corresponding to autolysins A and B were variable depending on the strains. This observation suggested that autolysins are highly conserved enzymes. A concentrated crude lysate of the virulent bacteriophage 832-B1 infecting L. helveticus was also analyzed by zymogram; one lytic activity with an apparent molecular weight of 31.7 kDa, very close to the weights of the autolysins B, was observed. Finally, the autolysins of L. helveticus ISLC5 were successfully extracted from whole cells by using a 1 M lithium chloride solution; they were partially purified by precipitation, selective resolubilization, and gel filtration chromatography, which led to a 20-fold increase in specific activity.

Valence, F.; Lortal, S.

1995-01-01

359

Protective effects of two Lactobacillus plantarum strains in hyperlipidemic mice  

PubMed Central

AIM: To investigate the effects of Lactobacillus plantarum (L. plantarum) CAI6 and L. plantarum SC4 on hyperlipidemic mice. METHODS: Male Kunming mice were fed a high-cholesterol diet for 28 d to construct hyperlipidemic models. Hyperlipidemic mice and normal mice were assigned to 3 groups which were separately treated with L. plantarum CAI6, L. plantarum SC4, and physiological saline through oral gavage for 28 d. Total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) levels were measured by commercially available enzyme kits. FACS Calibur flow cytometry was used to examine hepatic and renal nuclear factor-erythroid 2-related factor 2 (Nrf2) expression. The morphology of livers was checked by hematoxylin and eosin staining and optical microscope observation. RESULTS: Compared with normal mice, hyperlipidemic mice possessed significantly higher TC (3.50 ± 0.43 vs 2.89 ± 0.36, P < 0.01), TG (1.76 ± 0.07 vs 1.10 ± 0.16, P < 0.01), and LDL-C (1.72 ± 0.20 vs 0.82 ± 0.10, P< 0.01) levels, resulting in an increase of atherogenic index (AI) (2.34 ± 1.60 vs 0.93 ± 0.55, P < 0.05) and LDL-C/HDL-C ratio (1.43 ± 0.12 vs 0.51 ± 0.16, P < 0.05). After treatment with L. plantarum CAI6/L. plantarum SC4, TG (1.43 ± 0.27/1.54 ± 0.10 vs 1.76 ± 0.07, P < 0.01/P < 0.05) and LDL-C (1.42 ± 0.07/1.47 ± 0.12 vs 1.72 ± 0.20, P < 0.01/P < 0.01) in hyperlipidemic mice significantly decreased. In addition, TC, HDL-C, AI, and LDL-C/HDL-C ratio were all positively changed. Meanwhile, the treatment markedly alleviated hepatic steatosis and significantly stimulated Nrf2 expression (73.79 ± 0.80/72.96 ± 1.22 vs 54.94 ± 1.84, P < 0.01/P < 0.01) in hepatocytes of hyperlipidemic mice. CONCLUSION: L. plantarum CAI6 and L. plantarum SC4 may protect against cardiovascular disease by lipid metabolism regulation and Nrf2-induced antioxidative defense in hyperlipidemic mice.

Wang, Li-Xin; Liu, Kai; Gao, Da-Wei; Hao, Ji-Kui

2013-01-01

360

Helicobacter pylori eradication: Sequential therapy and Lactobacillus reuteri supplementation  

PubMed Central

AIM: To evaluate the role of sequential therapy and Lactobacillus reuteri (L. reuteri) supplementation, in the eradication treatment of Helicobacter pylori (H. pylori). METHODS: H. pylori infection was diagnosed in 90 adult dyspeptic patients. Patients were excluded if previously treated for H. pylori infection or if they were taking a proton pump inhibitor (PPI), H2-receptor antagonist or antibiotics. Patients were assigned to receive one of the following therapies: (1) 7-d triple therapy (PPI plus clarithromycin and amoxicillin or metronidazole) plus L. reuteri supplementation during antibiotic treatment; (2) 7-d triple therapy plus L. reuteri supplementation after antibiotic treatment; (3) sequential regimen (5-d PPI plus amoxicillin therapy followed by a 5-d PPI, clarithromycin and tinidazole) plus L. reuteri supplementation during antibiotic treatment; and (4) sequential regimen plus L. reuteri supplementation after antibiotic treatment. Successful eradication therapy was defined as a negative urea breath test at least 4 wk following treatment. RESULTS: Ninety adult dyspeptic patients were enrolled, and 83 (30 male, 53 female; mean age 57 ± 13 years) completed the study. Nineteen patients were administered a 7-d triple treatment: 11 with L. reuteri supplementation during and 8 after therapy. Sixty-four patients were administered a sequential regimen: 32 with L. reuteri supplementation during and 32 after therapy. The eradication rate was significantly higher in the sequential group compared with the 7-d triple regimen (88% vs 63%, P = 0.01). No difference was found between two types of PPI. No difference in eradication rates was observed between patients submitted to L. reuteri supplementation during or after antibiotic treatment. Compliance with therapy was excellent in all patients. No difference in adverse effects was observed between the different antibiotic treatments and between patients submitted to L. reuteri supplementation during and after antibiotic treatment. There was a low incidence of adverse effects in all groups of patients with sequential therapy, probably due to the presence of the L. reuteri supplementation. CONCLUSION: The sequential treatment regimen achieved a significantly higher eradication rate of H. pylori compared with standard 7-d regimen. L. reuteri supplementation could reduce the frequency and the intensity of antibiotic-associated side-effects.

Efrati, Cesare; Nicolini, Giorgia; Cannaviello, Claudio; O'Sed, Nicole Piazza; Valabrega, Stefano

2012-01-01

361

Environmental Interactions of Lactobacillus reuteri. Signal Transduction, Gene Expression and Extracellular Proteins of a Lactic Acid Bacterium.  

National Technical Information Service (NTIS)

The commensal bacterium Lactobacillus reuteri inhabits the human gastrointestinal tract and possesses putative probiotic, i.e. health-promoting, properties. In this thesis, features important for the ecological performance of L. reuteri and for interactio...

T. Wall

2005-01-01

362

Restoration of Normal Glutamic Acid Transport in Vitamin B6-Deficient Lactobacillus Plantarum by Acetate, Ammonium, and Vitamin B6.  

National Technical Information Service (NTIS)

A vitamin B6-deficiency in Lactobacillus plantarum markedly reduces the amount of glutamate and other amino acids which can be taken up and accumulated from buffered solutions. The capacity for glutamate accumulation is restored to normal levels when acet...

J. T. Holden

1964-01-01

363

Genome Sequence of Lactobacillus plantarum 19L3, a Strain Proposed as a Starter Culture for Slovensk? Bryndza Ovine Cheese  

PubMed Central

The genome sequence of Lactobacillus plantarum isolated from ovine cheese is presented here. This bacterium is proposed as a starter strain, named 19L3, for Slovenská bryndza cheese, a traditional Slovak cheese fulfilling European Food Safety Authority (EFSA) requirements.

Dzunkova, Maria; Moya, Andres; Tomaska, Martin; Kolosta, Miroslav; Kmet, Vladimir

2014-01-01

364

Short report: a placebo-controlled study of Lactobacillus GG colonization in one-to-three-year-old Peruvian children.  

PubMed

A pilot, placebo-controlled study conducted in Peruvian toddlers in a periurban shanty-town community demonstrates that 1) a simple fluorescent antibody test performed on bacteria from colonies grown on solid culture media can be used as a presumptive screening tool for Lactobacillus GG, 2) Lactobacillus GG powder sprinkled on flavored gelatin as a nutritional supplement is well-accepted by infants and mothers in this population, and 3) daily doses of Lactobacillus GG result in efficient colonization of the gastrointestinal tract of Peruvian infants. This study demonstrates that Lactobacillus GG should be evaluated as an adjunct for diarrhea control programs at the community level as well as in hospital-based settings. PMID:7771602

Sheen, P; Oberhelman, R A; Gilman, R H; Cabrera, L; Verastegui, M; Madico, G

1995-05-01

365

In vitro evaluation of the fermentation properties of galactooligosaccharides synthesised by a-galactosidase from Lactobacillus reuteri  

Microsoft Academic Search

Stirred, pH-controlled anaerobic batch cultures were used to evaluate the in vitro utilisation by canine gut microflora of novel a-galactooligosaccharides synthesised with an enzyme extract from a canine Lactobacillus reuteri strain. Fructooligosaccharides (FOS), melibiose and raffinose were used as reference carbohydrates for the prebiotic properties of the synthesised oligosaccharide (galactosyl melibiose mixture—GMM). Addition of Lactobacillus acidophilus was used as control

G. Tzortzis; A. K. Goulas; M.-L. A. Baillon; G. R. Gibson; R. A. Rastall

2004-01-01

366

Probiotic Lactobacillus Acidophilus and L. Casei Mix Sensitize Colorectal Tumoral Cells to 5-Fluorouracil-Induced Apoptosis  

Microsoft Academic Search

To assess the potential of Lactobacillus acidophilus and Lactobacillus casei strains to increase the apoptosis of a colorectal cancer cell line in the presence of 5-fluorouracil (5-FU), LS513 colorectal cancer cells were treated for 48 h with increasing concentrations of these lactic acid bacteria (LAB) in the presence of 100 ? g\\/ml of 5-FU. In the presence of 10 CFU\\/ml

Cindy Baldwin; Mathieu Millette; Daniel Oth; Marcia T. Ruiz; François-Marie Luquet; Monique Lacroix

2010-01-01

367

Detection and Identification of Gastrointestinal Lactobacillus Species by Using Denaturing Gradient Gel Electrophoresis and Species-Specific PCR Primers  

Microsoft Academic Search

Denaturing gradient gel electrophoresis (DGGE) of DNA fragments obtained by PCR amplification of the V2-V3 region of the 16S rRNA gene was used to detect the presence of Lactobacillus species in the stomach contents of mice. Lactobacillus isolates cultured from human and porcine gastrointestinal samples were identified to the species level by using a combination of DGGE and species-specific PCR

J. Walter; G. W. Tannock; A. Tilsala-Timisjarvi; S. Rodtong; D. M. Loach; K. Munro; T. Alatossava

2000-01-01

368

Microstructure and Physical Properties of a Reduced Fat Mozzarella Cheese Made Using Lactobacillus casei ssp. casei Adjunct Culture  

Microsoft Academic Search

Reduced fat Mozzarella cheese was produced usingStreptococcus thermophilusandLactobacillus helveticus, and either total or partial replacement ofL. helveticuswithLactobacillus caseissp.casei. Stretch, melt and cook colour were determined at 1, 7, 14, and 28 d. All cheeses decreased in stretch during the first 7 d of storage, but there were no significant differences between culture types. Reduced fat cheese made withL. helveticusandS. thermophilusshowed

Richard K. Merrill; Craig J. Oberg; William R. McManus; Miloslav Kalab; Donald J. McMahon

1996-01-01

369

The Increase of Lactobacillus Species in the Gut Flora of Newborn Broiler Chicks and Ducks Is Associated with Weight Gain  

PubMed Central

Background A bacterial role in the obesity pandemic has been suspected based on the ingestion of probiotics that can modify the gut flora. The objective of our study was to determine if increased Lactobacillus sp. in the gut flora of newborn broiler chicks and ducks could result in weight gain increase. Methodology Female broiler chicks (Gallus gallus domesticus) and ducks (Anas platyrhynchos domestica) were separated into one control and two experimental groups, and inoculated once or twice with 4×1010 Lactobacillus spp. per animal in PBS, or with PBS alone. Fecal samples were collected before and at 24 hours, 2, 4, 8, 16 and 30 days after the inoculation. DNA was extracted from the stools, and qPCR assays were performed on a MX3000™ system for the detection and quantification of Lactobacillus sp., Bacteroidetes and Firmicutes, using a quantification plasmid. Animals were measured and sacrificed 60 days after the beginning of the experiment, and livers were collected and measured. Principal Findings Chicks inoculated once and twice with Lactobacillus weighed 10.2% (p?=?0.0162) and 13.5% (p?=?0.0064) more than the control group animals, respectively. Similarly, ducks inoculated once and twice weighed 7.7% (p?=?0.05) and 14% (p?=?0.035) more than those in the control group, respectively. Liver mass was also significantly higher in inoculated animals compared to the control group. Inoculation with Lactobacillus sp. increased the DNA copies of Lactobacillus spp. and Firmicutes in the stools. Bacteroidetes remained stable, and only the second Lactobacillus sp. inoculation significantly decreased its population in chicks. The ratio of DNA copies of Firmicutes to those of Bacteroidetes increased to as much as 6,4 in chicks and 8,3 in ducks. Conclusions Differences in the intestinal microbiota may precede weight increase, as we found that an increase of Lactobacillus sp. in newborn ducks and chicks preceded the development of weight gain.

Angelakis, Emmanouil; Raoult, Didier

2010-01-01

370

rRNA gene restriction patterns as a characterization tool for Lactobacillus sake strains producing ropy slime  

Microsoft Academic Search

The rRNA gene restriction patterns (ribotypes) of 69 ropy slime producing Lactobacillus sake strains isolated mainly from vacuum-packaged meat products of ten meat plants were determined. Ribotypes were compared to the corresponding patterns of non-ropy L. sake strains, and also to other species of the genus Lactobacillus, Carnobacterium and Weissella associated with meat products. Ropy slime-producing L. sake strains were

Johanna Björkroth; Hannu Korkeala

1996-01-01

371

Analysis ofLactobacillusPhages and Bacteriocins in American Dairy Products and Characterization of a Phage Isolated from Yogurt  

Microsoft Academic Search

Yogurt and acidophilus milk that containLactobacillus acidophiluscould promote human health becauseL. acidophilus can inhibit enteric and food-borne microbial pathogens. To evaluate the stability of dairy L. acidophilus cultures, we studied whether some dairy lactobacilli could be inhibited by phages or bacteriocins released by other dairy lactobacilli. From 20 yogurts and two acidophilus milks purchased at local food markets, 38 Lactobacillus

ALI O. KILIC; SYLVIA I. PAVLOVA; WEN-GE MA; ANDLIN TAO

1996-01-01

372

In Vitro Prevention of Salmonella Lipopolysaccharide-Induced Damages in Epithelial Barrier Function by Various Lactobacillus Strains  

PubMed Central

Background. Lactobacillus shows beneficial anti-inflammatory effects on Salmonella infection. The maintenance of the tight junction (TJ) integrity plays an importance role in avoiding bacterial invasion. Whether Lactobacillus could be used to regulate the TJ protein expression and distribution in inflamed intestinal epithelial cells was determined. Methods. Using the transwell coculture model, Salmonella lipopolysaccharide (LPS) was apically added to polarized Caco-2 cells cocultured with peripheral blood mononuclear cells in the basolateral compartment. LPS-stimulated Caco-2 cells were incubated with various Lactobacillus strains. TJ integrity was determined by measuring transepithelial electrical resistance across Caco-2 monolayer. Expression and localization of TJ proteins (zonula-occludens- (ZO-) 1) were determined by Western blot and immunofluorescence microscopy. Results. Various strains of Lactobacillus were responsible for the different modulations of cell layer integrity. LPS was specifically able to disrupt epithelial barrier and change the location of ZO-1. Our data demonstrate that Lactobacillus could attenuate the barrier disruption of intestinal epithelial cells caused by Salmonella LPS administration. We showed that Lactobacillus strains are associated with the maintenance of the tight junction integrity and appearance. Conclusion. In this study we provide insight that live probiotics could improve epithelial barrier properties and this may explain the potential mechanism behind their beneficial effect in vivo.

Chiang Chiau, Jen-Shiu; Chan, Wai-Tao; Jiang, Chun-Bin; Cheng, Mei-Lien; Liu, Hsuan-Liang

2013-01-01

373

Construction of a new reporter system to study the NaCl-dependent dnaK promoter activity of Lactobacillus sanfranciscensis  

Microsoft Academic Search

A reporter system was developed to study gene expression in Lactobacillus sanfranciscensis. It was based on the Escherichia coli\\/Lactobacillus shuttle vector pLP3537 and the melA gene encoding ?-galactosidase originating from Lactobacillus plantarum. melA was functionally expressed in E. coli and L. sanfranciscensis, and activity was easily monitored in vivo as well as in vitro by applying an optimized enzyme assay.

Sebastian Hörmann; Rudi F. Vogel; Matthias Ehrmann

2006-01-01

374

Growth phase of orally administered Lactobacillus strains differentially affects IgG1\\/IgG2a ratio for soluble antigens: implications for vaccine development  

Microsoft Academic Search

Lactobacillus strains with probiotic activity are major constituents of numerous common food products. Due to their ‘generally regarded as safe’-status (GRAS-status), Lactobacillus strains can also be genetically engineered for use in oral immunotherapeutic applications, such as vaccination and T lymphocyte tolerance induction in autoimmune disease.In the current study, we demonstrate that the growth phase of orally administered individual Lactobacillus strains

Catharina B. M. Maassen; Wim J. A. Boersma; Conny van Holten-Neelen; Eric Claassen; Jon D. Laman

2003-01-01

375

Genotypic and phenotypic characterization of lactic acid bacteria isolated from Italian ryegrass silage.  

PubMed

Twenty-three lactic acid bacteria (LAB) isolated from three cultivars (Akiaoba, Nagahahikari and Tachiwase) of Italian ryegrass (Lolium multiflorum Lam.) silage were precisely characterized by a combination of phenotypic tests, genotypic 16S ribosomal DNA sequencing and rapid PCR-based analyses, focusing on their useful phenotypes for silage preparation as inoculants. We successfully identified both at the species and subspecies levels: phenotypically novel Lactococcus lactis subsp. lactis, Lactobacillus brevis, Lactobacillus coryniformis subsp. torquens, Lactobacillus curvatus, Lactobacillus plantarum subsp. plantarum, Lactobacillus sakei subsp. carnosus, Leuconostoc mesenteroides subsp. dextranicum and Pediococcus parvulus. This is the first report to elucidate the presence of Lactobacillus coryniformis ssp. torquens and Leuconostoc mesenteroides subsp. dextranicum in Italian ryegrass silages. Physiological and biochemical tests revealed that phenotypic characteristics are different among the different strains of the same species and subspecies, and that the isolates show unique and diverse phenotypes related to fermentation factors, such as available carbohydrates, optimal growth pH and temperature. These results suggest that, for various well-preserved silage preparations, the isolates may be useful in producing novel inoculants corresponding to their optimally climatic and ecological niches. PMID:22339691

Tohno, Masanori; Kobayashi, Hisami; Nomura, Masaru; Kitahara, Maki; Ohkuma, Moriya; Uegaki, Ryuichi; Cai, Yimin

2012-02-01

376

A mild pulsed electric field condition that improves acid tolerance, growth, and protease activity of Lactobacillus acidophilus LA-K and Lactobacillus delbrueckii subspecies bulgaricus LB-12.  

PubMed

Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than 1 s to fluid products placed between 2 electrodes. The effect of mild PEF on beneficial characteristics of probiotic bacteria Lactobacillus acidophilus and Lactobacillus delbrueckii ssp. bulgaricus is not clearly understood. The objective of this study was to determine the influence of mild PEF conditions on acid tolerance, growth, and protease activity of Lb. acidophilus LA-K and Lactobacillus delbrueckii ssp. bulgaricus LB-12. A pilot plant PEF system (OSU-4M; The Ohio State University, Columbus) was used. The PEF treatments were positive square unipolar pulse width of 3 µs, pulse period of 0.5s, electric field strength of 1 kV/cm, delay time of 20 µs, flow rate of 60 mL/min, and 40.5°C PEF treatment temperature. Both Lb. acidophilus LA-K and Lb. bulgaricus LB-12 subjected to mild PEF conditions were acid tolerant until the end of the 120 min of incubation, unlike the Lb. bulgaricus control, which was not acid tolerant after 30 min. The mild PEF-treated Lb. acidophilus LA-K and Lb. bulgaricus LB-12 reached the logarithmic phase of growth an hour earlier than the control. Mild PEF conditions studied significantly improved acid tolerance, exponential growth, and protease activity of both Lb. acidophilus LA-K and Lb. bulgaricus LB-12 compared with the control. The mild PEF conditions studied can be recommended for pretreating cultures to enhance these desirable attributes. PMID:23587394

Najim, N; Aryana, Kayanush J

2013-06-01

377

Lactobacillus rhamnosus L34 and Lactobacillus casei L39 suppress Clostridium difficile-induced IL-8 production by colonic epithelial cells  

PubMed Central

Background Clostridium difficile is the main cause of hospital-acquired diarrhea and colitis known as C. difficile-associated disease (CDAD).With increased severity and failure of treatment in CDAD, new approaches for prevention and treatment, such as the use of probiotics, are needed. Since the pathogenesis of CDAD involves an inflammatory response with a massive influx of neutrophils recruited by interleukin (IL)-8, this study aimed to investigate the probiotic effects of Lactobacillus spp. on the suppression of IL-8 production in response to C. difficile infection. Results We screened Lactobacillus conditioned media from 34 infant fecal isolates for the ability to suppress C. difficile-induced IL-8 production from HT-29 cells. Factors produced by two vancomycin-resistant lactobacilli, L. rhamnosus L34 (LR-L34) and L.casei L39 (LC-L39), suppressed the secretion and transcription of IL-8 without inhibiting C. difficile viability or toxin production. Conditioned media from LR-L34 suppressed the activation of phospho-NF-?B with no effect on phospho-c-Jun. However, LC-L39 conditioned media suppressed the activation of both phospho-NF-?B and phospho-c-Jun. Conditioned media from LR-L34 and LC-L39 also decreased the production of C. difficile-induced GM-CSF in HT-29 cells. Immunomodulatory factors present in the conditioned media of both LR-L34 and LC-L39 are heat-stable up to 100°C and?>?100 kDa in size. Conclusions Our results suggest that L. rhamnosus L34 and L. casei L39 each produce factors capable of modulating inflammation stimulated by C. difficile. These vancomycin-resistant Lactobacillus strains are potential probiotics for treating or preventing CDAD.

2014-01-01

378

Isolation of a virulent Lactobacillus brevis phage and its application in the control of beer spoilage.  

PubMed

Beer quality can be compromised by the growth of certain lactobacilli, in particular Lactobacillus brevis and Lactobacillus plantarum, and various strategies have been used to control such bacterial spoilage. Biocontrol by means of bacteriophage is a reemerging approach for the suppression of spoilage bacteria in food and beverage matrices. A virulent phage capable of infecting L. brevis beer-spoilage strains was isolated and morphologically assessed by electron microscopy. The myophage SA-C12 was shown to be stable in beer and capable of controlling the growth of its host, L. brevis strain 56, in commercial beer. The results of this study indicate that bacteriophage-based treatments may be used as an alternative and natural strategy for the control of bacterial contamination of beer. PMID:22186058

Deasy, Therese; Mahony, Jennifer; Neve, Horst; Heller, Knut J; van Sinderen, Douwe

2011-12-01

379

Purification and characterization of plantaricin Y, a novel bacteriocin produced by Lactobacillus plantarum 510.  

PubMed

Lactobacillus plantarum 510, previously isolated from a koshu vineyard in Japan, was found to produce a bacteriocin-like inhibitory substance which was purified and characterized. Mass spectrometry analysis showed that the mass of this bacteriocin is 4,296.65 Da. A partial sequence, NH2- SSSLLNTAWRKFG, was obtained by N-terminal amino acid sequence analysis. A BLAST search revealed that this is a unique sequence; this peptide is thus a novel bacteriocin produced by Lactobacillus plantarum 510 and was termed plantaricin Y. Plantaricin Y shows strong inhibitory activity against Listeria monocytogenes BCRC 14845, but no activity against other pathogens tested. Bacteriocin activity decreased slightly after autoclaving (121 °C for 15 min), but was completely inactivated by protease K. Furthermore, trypsin-digested bacteriocin product fragments retained activity against L. monocytogenes BCRC 14845 and exhibited a different inhibitory spectrum. PMID:24493293

Chen, Yi-sheng; Wang, Yan-chong; Chow, Yiou-shing; Yanagida, Fujitoshi; Liao, Chen-chung; Chiu, Chi-ming

2014-03-01

380

Protective efficacy of orally administered, heat-killed Lactobacillus pentosus b240 against influenza A virus.  

PubMed

Influenza A(H1N1)pdm virus caused the first human pandemic of the 21st century. Although various probiotic Lactobacillus species have been shown to have anti-microbial effects against pneumonia-inducing pathogens, the prophylactic efficacy and mechanisms behind their protection remain largely unknown. Here, we evaluated the prophylactic efficacy of heat-killed Lactobacillus pentosus b240 against lethal influenza A(H1N1)pdm virus infection in a mouse model. To further define the protective responses induced by b240, we performed virologic, histopathologic, and transcriptomic analyses on the mouse lungs. Although we did not observe an appreciable effect of b240 on virus growth, cytokine production, or histopathology, gene expressional analysis revealed that oral administration of b240 differentially regulates antiviral gene expression in mouse lungs. Our results unveil the possible mechanisms behind the protection mediated by b240 against influenza virus infection and provide new insights into probiotic therapy. PMID:23535544

Kiso, Maki; Takano, Ryo; Sakabe, Saori; Katsura, Hiroaki; Shinya, Kyoko; Uraki, Ryuta; Watanabe, Shinji; Saito, Hiroshi; Toba, Masamichi; Kohda, Noriyuki; Kawaoka, Yoshihiro

2013-01-01

381

Protective efficacy of orally administered, heat-killed Lactobacillus pentosus b240 against influenza A virus  

PubMed Central

Influenza A(H1N1)pdm virus caused the first human pandemic of the 21st century. Although various probiotic Lactobacillus species have been shown to have anti-microbial effects against pneumonia-inducing pathogens, the prophylactic efficacy and mechanisms behind their protection remain largely unknown. Here, we evaluated the prophylactic efficacy of heat-killed Lactobacillus pentosus b240 against lethal influenza A(H1N1)pdm virus infection in a mouse model. To further define the protective responses induced by b240, we performed virologic, histopathologic, and transcriptomic analyses on the mouse lungs. Although we did not observe an appreciable effect of b240 on virus growth, cytokine production, or histopathology, gene expressional analysis revealed that oral administration of b240 differentially regulates antiviral gene expression in mouse lungs. Our results unveil the possible mechanisms behind the protection mediated by b240 against influenza virus infection and provide new insights into probiotic therapy.

Kiso, Maki; Takano, Ryo; Sakabe, Saori; Katsura, Hiroaki; Shinya, Kyoko; Uraki, Ryuta; Watanabe, Shinji; Saito, Hiroshi; Toba, Masamichi; Kohda, Noriyuki; Kawaoka, Yoshihiro

2013-01-01

382

Utilization of Lactobacillus sp. for steroid glycoalkaloids degradation by lactic acid fermentation.  

PubMed

The degradation fo steroid glycoalkaloids (SGAs) has been studied in model solutions. The number of colony forming units (CFU) was determined using a nondirect (cultivation) method during all stages of fermentation. The changes in SGAs content were observed by HPLC on the Supelcosil LC-NH2 column. The changes in alpha-tomatine concentration added to fermented Lactobacillus MRS broth have been studied. A mathematical model of steroid glycoalkaloids degradation during lactic fermentation was proposed. The mathematical model was based on the experimental data of SGA and glucose concentration and should be used for study and prediction of SGA concentration changes of fermented samples. The ratio of SGA degradation rate by fermentation and by lactic acid hydrolysis was calculated. The experimental data evaluated by proposed mathematical model for the selected strain of Lactobacillus plantarum 976H show real feasibility for SGA degradation by lactic acid fermentation. PMID:12224420

Veselá, Maria; Drdák, M; Simon, P; Veselý, M

2002-08-01

383

New integration vector using a cellulase gene as a screening marker for Lactobacillus.  

PubMed

The new integration vector for Lactobacillus, pJC4, was developed using the extracellular endoglucanase A gene (celA) of Clostridium thermocellum as a screening marker. pJC4 was transformed into four Lactobacillus species, Lb. johnsonii, Lb. gasseri, Lb. bulgaricus, and Lb. plantarum. In each species, the pJC4 integrants were easily and accurately detected by the appearance of a clear halo on a cellulase screening plate without any false transformants. Polymerase chain reaction and Southern hybridization indicated that all transformants with clear halos contained pJC4 in their chromosomal DNAs. The celA gene could be a useful screening marker for other lactic acid bacteria. PMID:12892882

Jang, Soo Jin; Ham, Min Seok; Lee, Jung Min; Chung, Sung Kyun; Lee, Hyong Joo; Kim, Jeong Hwan; Chang, Hae Choon; Lee, Jong-Hoon; Chung, Dae Kyun

2003-07-29

384

A food additive with prebiotic properties of an ?-d-glucan from Lactobacillus plantarum DM5.  

PubMed

An ?-d-glucan produced by Lactobacillus plantarum DM5 was explored for in vitro prebiotic activities. Glucan-DM5 demonstrated 21.6% solubility, 316.9% water holding capacity, 86.2% flocculation activity, 71.4% emulsification activity and a degradation temperature (Td) of 292.2°C. Glucan-DM5 exhibited lowest digestibility of 0.54% by artificial gastric juice, 0.21% by intestinal fluid and 0.32% by ?-amylase whereas the standard prebiotic inulin, showed 25.23%, 5.97% and 19.13%, hydrolysis, respectively. Prebiotic activity assay of glucan-DM5 displayed increased growth of probiotic bacteria such as Bifidobacterium infantis and Lactobacillus acidophilus, but did not support the growth of non-probiotic bacteria such as Escherichia coli and Enterobacter aerogenes. The overall findings indicated that glucan from L. plantarum DM5 can serve as a potential prebiotic additive for food products. PMID:24857877

Das, Deeplina; Baruah, Rwivoo; Goyal, Arun

2014-08-01

385

Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate.  

PubMed Central

The emergence of acquired high-level resistance among Enterococcus species has renewed interest in mechanisms of resistance to glycopeptide antibiotics in gram-positive bacteria. In Enterococcus faecalis and Enterococcus faecium, resistance is encoded by the van gene cluster and is due to the production of a peptidoglycan precursor terminating in D-alanyl-D-lactate, to which vancomycin does not bind. Most Leuconostoc and many Lactobacillus species are intrinsically resistant to high levels of glycopeptide antibiotics, but the mechanism of resistance has not been elucidated. To determine whether the mechanisms of resistance are similar in intrinsically resistant bacteria, cytoplasmic peptidoglycan precursors were isolated from Leuconostoc mesenteroides and Lactobacillus casei and analyzed by mass spectrometry, revealing structures consistent with UDP-N-acetylmuramyl-L-Ala-D-Glu-L-Lys-(L-Ala)-D-Ala-D-lactate and UDP-N-acetylmuramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, respectively.

Handwerger, S; Pucci, M J; Volk, K J; Liu, J; Lee, M S

1994-01-01

386

Oat ?-glucan and xylan hydrolysates as selective substrates for Bifidobacterium and Lactobacillus strains  

Microsoft Academic Search

Novel oligomers that resist digestion in the upper gut were prepared from oat mixed-linked ?-glucan and xylan by enzymatic\\u000a hydrolysis with lichenase of Bacillus subtilis and xylanase of Trichoderma reesei respectively. The low-molecular-mass hydrolysis products of ?-glucan and xylan were compared with fructooligomers and raffinose\\u000a in their ability to provide growth substrates for probiotic (Lactobacillus and Bifidobacterium) and intestinal (Bacteroides,

J. Jaskari; P. Kontula; A. Siitonen; H. Jousimies-Somer; T. Mattila-Sandholm; K. Poutanen

1998-01-01

387

Applicability of rep-PCR fingerprinting for identification of Lactobacillus species  

Microsoft Academic Search

PCR amplification of repetitive bacterial DNA elements fingerprinting using the (GTG)5 primer ((GTG)5-PCR) was proven to be useful for differentiation of a wide range of lactobacilli (i.e. 26 different (sub)species) at the species, subspecies and potentially up to the strain level. Using this rapid and reproducible genotypic technique, new Lactobacillus isolates recovered from different types of fermented dry sausage could

Dirk Gevers; Geert Huys; Jean Swings

2001-01-01

388

Lactobacillus species prevents colitis in interleukin 10 gene–deficient mice  

Microsoft Academic Search

Background & Aims: Intestinal luminal microflora, or their products, are likely an important initiating factor in the pathogenesis of inflammatory bowel disease. The aim of this study was to determine the role of colonic aerobic luminal bacteria and Lactobacillus species in the development of colitis in interleukin (IL)-10 gene–deficient mice. Methods: Intestine from 2–16-week-old mice was scored histologically and cultured

Karen L. Madsen; Jason S. Doyle; Laurence D. Jewell; Michele M. Tavernini; Richard N. Fedorak

1999-01-01

389

Improved immunogenicity of oral D x RRV reassortant rotavirus vaccine by Lactobacillus casei GG  

Microsoft Academic Search

In a search for new strategies to improve oral vaccination, the effect of orally administered Lactobacillus casei strain GG (LGG) in conjunction with D x RRV rhesus-human reassortant live oral rotavirus vaccine was tested in 2–5-month-old infants. Infants who received LGG showed an increased response with regard to rotavirus-specific IgM secreting cells, measured using an ELISPOT technique, on day 8

Erika Isolauri; Jaana Joensuu; Hanna Suomalainen; Mari Luomala; Timo Vesikari

1995-01-01

390

Molecular Analysis of the Composition of the Bifidobacterial and Lactobacillus Microflora of Humans  

Microsoft Academic Search

Thebifidobacterialandlactobacilluspopulationsoffecalsamplescollectedfromtwohumansubjectsduring a 12-month period were studied. The total numbers of bifidobacteria were stable throughout the study period in both subjects, but lactobacillus numbers were less constant. Analysis of the composition of the bifidobac- terial populations by using ribotyping or pulsed-field gel electrophoresis to differentiate between bacterial strains demonstrated major differences between the subjects. Subject 1 harboredfive strains of bifidobacteria throughoutthe12-monthperiod,andonestrainwasnumericallypredominant.Incontrast,subject2harbored

ANNE L. MCCARTNEY; WANG WENZHI; ANDGERALD W. TANNOCK

1996-01-01

391

The Effect of Oral Feeding of Lactobacillus casei Strain Shirota on Immunoglobulin E Production in Mice  

Microsoft Academic Search

We investigated the effect of oral feeding of heat- killed Lactobacillus casei strain Shirota on im- munoglobulin E (IgE) production in mice. The strain was orally administered to BALB\\/c mice that had been preinjected intraperitoneally with ovalbumin, and the level of IgE in serum was determined. Results indicated that the oral feeding of L. casei strain Shirota was effective in

T. Matsuzaki; R. Yamazaki; S. Hashimoto; T. Yokokura

1998-01-01

392

Transformation of Lactobacillus reuteri with electroporation: Studies on the erythromycin resistance plasmid pLUL631  

Microsoft Academic Search

A high-frequency transformation system was developed forLactobacillus reuteri with the electroporation technique. With this method, transformation frequencies of 107 transformants per µg DNA were routinely obtained with the plasmid pLUL631 and its derivatives. pLUL631, a nativeL. reuteri plasmid containing an erythromycin resistance determinant, was studied mainly with regard to replicon size and stability. The minimal replicon of the plasmid was

Siv Ahrné; Göran Molin; Lars Axelsson

1992-01-01

393

Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium  

Microsoft Academic Search

Fructosyltransferases, like the Lactobacillus reteri levansucrase, are important for the production of new fructosyloligosaccharides. Various His6- and Strep-tagged variants of this enzyme were recombinantly produced and exported into the growth medium using the Gram-positive\\u000a bacterium Bacillus megaterium. Nutrient-rich growth medium significantly enhanced levansucrase production and export. The B. megaterium signal peptide of the extracellular esterase LipA mediated better levansucrase export

Rebekka Biedendieck; Rafael Beine; Martin Gamer; Eva Jordan; Klaus Buchholz; Jürgen Seibel; Lubbert Dijkhuizen; Marco Malten; Dieter Jahn

2007-01-01

394

Production of 3-hydroxypropionaldehyde using a two-step process with Lactobacillus reuteri  

Microsoft Academic Search

3-Hydroxypropionaldehyde (3-HPA) produced by Lactobacillus reuteri is a broad-spectrum antimicrobial substance of glycerol conversion. The aim of the present work was to optimize 3-HPA production\\u000a by Lb. reuteri ATCC 53608 using a two-step process. The first step was the production of Lb. reuteri cells in optimal conditions. Cells were then harvested by centrifugation and suspended in glycerol solution, which the

Y. Doleyres; P. Beck; S. Vollenweider; C. Lacroix

2005-01-01

395

Human-derived probiotic Lactobacillus reuteri demonstrate antimicrobial activities targeting diverse enteric bacterial pathogens  

Microsoft Academic Search

Lactobacillus reuteri is a commensal-derived anaerobic probiotic that resides in the human gastrointestinal tract. L. reuteri converts glycerol into a potent broad-spectrum antimicrobial compound, reuterin, which inhibits the growth of gram-positive and gram-negative bacteria. In this study, we compared four human-derived L. reuteri isolates (ATCC 55730, ATCC PTA 6475, ATCC PTA 4659 and ATCC PTA 5289) in their ability to

Jennifer K. Spinler; Malai Taweechotipatr; Cheryl L. Rognerud; Ching N. Ou; Somying Tumwasorn; James Versalovic

2008-01-01

396

Synthesis of a-galactooligosaccharides with a-galactosidase from Lactobacillus reuteri of canine origin  

Microsoft Academic Search

Crude cell-free extracts from Lactobacillus reuteri grown on cellobiose, maltose, lactose and raffinose were assayed for glycosidic activities. When raffinose was used as the carbon source, a-galactosidase was produced, showing the highest yield at the beginning of the stationary growth phase. A 64 kDa enzyme was purified by ultra- and gel filtration, and characterized for its hydrolytic and synthetic activity. Highest

G. Tzortzis; A. J. Jay; M. L. A. Baillon; G. R. Gibson; R. A. Rastall

2003-01-01

397

Lactobacillus reuteri in bovine milk fermented decreases the oral carriage of mutans streptococci  

Microsoft Academic Search

The effect of Lactobacillus reuteri against one of the major cariogenic organism, Streptococcus mutans, was studied. Yogurt products containing L.reuteri showed a significant growth inhibitory effect against S. mutans, whilst yoghurts with lactobaccilli other than L. reuteri did not show such inhibition. Further, double-blind, placebo-controlled trial demonstrated that consuming yogurt with L. reuteri significantly reduced the oral carriage of mutans

H. Nikawa; S. Makihira; H. Fukushima; H. Nishimura; Y. Ozaki; K. Ishida; S. Darmawan; T. Hamada; K. Hara; A. Matsumoto; T. Takemoto; R. Aimi

2004-01-01

398

Microbiological and sensory quality of dry fermented sausages containing alginate-microencapsulated Lactobacillus reuteri  

Microsoft Academic Search

The probiotic bacterium Lactobacillus reuteri was added to dry sausage batter, without or after being microencapsulated in alginate using either extrusion or emulsion technology. Pediococcus pentosaceus and Staphylococcus carnosus were added at 7 log cfu\\/g as starter cultures for fermentation. The sausage batter was stuffed in 55 mm fibrous casings and fermented, with smoking, at ?26 °C and 88% relative humidity (RH) for

Parthiban Muthukumarasamy; Richard A. Holley

2006-01-01

399

Purification of collagen-binding proteins of Lactobacillus reuteri NCIB 11951  

Microsoft Academic Search

Collagen type-I-binding proteins ofLactobacillus reuteri NCIB 11951 were purified. The cell surface proteins were affinity purified on collagen Sepharose and eluted with an NaCl gradient. Two protein bands were eluted from the column (29 kDa and 31 kDa), and both bound radio-labeled collagen type I. Rabbit antisera raised against the 29 kDa and 31 kDa protein reacted with the affinity-purified

P. Aleljung; W. Shen; B. Rozalska; U. Hellman; Å. Ljungh; T. Wadström

1994-01-01

400

Effect of membrane lateral pressure on the expression of fructosyltransferases in Lactobacillus reuteri  

Microsoft Academic Search

The effect of environmental conditions on the production of homo-polysaccharides and oligosaccharides from sucrose and the regulation of glycosyltransferase genes responsible for biosynthesis of homo-polysaccharides was determined in Lactobacillus reuteri TMW1.106 (reutericyclin-producer) and LTH5448 (reutericyclin-negative). Strain L. reuteri TMW 1.106 harbours the glycosyltransferase genes gtfA and inu, strain LTH5448 harbours a fructosyltransferase, ftfA. Fructan and fructose-oligosaccharide (FOS) production in both

Clarissa Schwab; Michael G. Gänzle

2006-01-01

401

Expression of Rumen Microbial Fibrolytic Enzyme Genes in Probiotic Lactobacillus reuteri  

Microsoft Academic Search

This study was aimed at evaluating the cloning and expression of three rumen microbial fibrolytic enzyme genes in a strain of Lactobacillus reuteri and investigating the probiotic characteristics of these genetically modified lactobacilli. The Neocallimastix patriciarum xylanase gene xynCDBFV, the Fibrobacter succinogenes -glucanase (1,3-1,4--D-glucan 4-glucanohydrolase (EC 3.2.1.73)) gene, and the Piromyces rhizinflata cellulase gene eglA were cloned in a strain

Je-Ruei Liu; Bi Yu; Fu-Hwa Liu; Kuo-Joan Cheng; Xin Zhao

2005-01-01

402

Effect of Lactobacillus reuteri on the Prevention of Hypercholesterolemia in Mice  

Microsoft Academic Search

Administration of Lactobacillus reuteri CRL 1098 (104 cells\\/d) to mice for 7 d before inducing hypercholesterol- emia (by feeding mice with a fat-enriched diet for the subsequent 7 d) was evaluated. At this low dose, L. reu- teri was effective in preventing hypercholesterolemia in mice, producing a 17% increase in the ratio of high- density lipoprotein to low-density lipoprotein. Total

M. P. Taranto; M. Medici; G. Perdigon; A. P. Ruiz Holgado; G. F. Valdez

2000-01-01

403

Pseudovitamin B 12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions  

Microsoft Academic Search

We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Co?-[?-(7-adenyl)]-Co?-cyanocobamide or pseudovitamin B12. This

Filipe Santos; José L. Vera; Pedro Lamosa; Graciela F. de Valdez; Willem M. de Vos; Helena Santos; Fernando Sesma; Jeroen Hugenholtz

2007-01-01

404

Protective effect of the bile salt hydrolase-active Lactobacillus reuteri against bile salt cytotoxicity  

Microsoft Academic Search

Bacterial bile salt hydrolysis is considered a risk factor for the development of colon cancer because of the risk of forming\\u000a harmful secondary bile salts after an initial deconjugation step. In this study, the influence of enhanced bacterial bile\\u000a salt transformation by the bile salt hydrolase-active Lactobacillus reuteri was studied in batch culture using the microbial suspension of the Simulator

P. De Boever; R. Wouters; L. Verschaeve; P. Berckmans; G. Schoeters; W. Verstraete

2000-01-01

405

Inhibition of binding of Helicobacter pylori to the glycolipid receptors by probiotic Lactobacillus reuteri  

Microsoft Academic Search

We examined the competition of binding of Lactobacillus reuteri and Helicobacter pylori to gangliotetraosylceramide (asialo-GM1) and sulfatide which are putative glycolipid receptor molecules of H. pylori, and identified a possible sulfatide-binding protein of the L. reuteri strain. Among nine L. reuteri strains, two (JCM1081 and TM105) were shown to bind to asialo-GM1 and sulfatide, and to inhibit binding of H.

Takao Mukai; Tomoko Asasaka; Eri Sato; Kenichi Mori; Mitsuyo Matsumoto; Hitoshi Ohori

2002-01-01

406

Screening and characterization of Lactobacillus strains producing large amounts of exopolysaccharides  

Microsoft Academic Search

A total of 182 Lactobacillus strains were screened for production of extracellular polysaccharides (EPS) by a new method: growth in liquid media with\\u000a high sugar concentrations. Sixty EPS-positive strains were identified; 17 strains produced more than 100?mg\\/l soluble EPS.\\u000a Sucrose was an excellent substrate for abundant EPS synthesis. The ability to produce glucans appears to be widespread in\\u000a the genus

G. H. van Geel-Schutten; F. Flesch; B. ten Brink; M. R. Smith; L. Dijkhuizen

1998-01-01

407

Structural characterisation of the exopolysaccharide produced by Lactobacillus delbrückii subspecies bulgaricus rr grown in skimmed milk  

Microsoft Academic Search

The exopolysaccharide of Lactobacillus delbrückii subsp. bulgaricus rr. isolated from skimmed milk, is a heteropolymer of D-galactopyranosyl, D-glucopyranosyl, and L-rhamnopyranosyl residues in the molar ratio 5:1:1. The structure was established by linkage analysis and 1D and 2D NMR spectroscopy of the native polysaccharide, in combination with characterisation of oligosaccharide fragments, obtained by Smith degradation and partial acid hydrolysis, using methylation

J. F. G. Vliegenthart; M. Gruter; B. R. Leeflang; J. Kuiper; J. P. Kamerling

1993-01-01

408

Effect of Lactobacillus plantarum and chitosan in the reduction of browning of pericarp Rambutan ( Nephelium lappaceum)  

Microsoft Academic Search

The effects of Lactobacillus plantarum alone or in combination with chitosan were evaluated on quality and color retention in rambutan fruits (Nephelium lappaceum) stored at 25°C and 10°C with 75±2.5% of relative humidity for 10 and 15 days, respectively. The development of the microorganisms was evidenced by viability analyses and lactic acid production. The application of L. plantarum significantly improved

Gustavo Martínez-Castellanos; Keiko Shirai; Clara Pelayo-Zaldívar; Laura J. Pérez-Flores; José D. Sepúlveda-Sánchez

2009-01-01

409

Coexpression and Secretion of Endoglucanase and Phytase Genes in Lactobacillus reuteri.  

PubMed

A multifunctional transgenic Lactobacillus with probiotic characteristics and an ability to degrade ?-glucan and phytic acid (phytate) was engineered to improve nutrient utilization, increase production performance and decrease digestive diseases in broiler chickens. The Bacillus subtilis WL001 endoglucanase gene (celW) and Aspergillus fumigatus WL002 phytase gene (phyW) mature peptide (phyWM) were cloned into an expression vector with the lactate dehydrogenase promoter of Lactobacillus casei and the secretion signal peptide of the Lactococcus lactis usp45 gene. This construct was then transformed into Lactobacillus reuteri XC1 that had been isolated from the gastrointestinal tract of broilers. Heterologous enzyme production and feed effectiveness of this genetically modified L. reuteri strain were investigated and evaluated. Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed that the molecular mass of phyWM and celW was approximately 48.2 and 55 kDa, respectively, consistent with their predicted molecular weights. Endoglucanase and phytase activities in the extracellular fraction of the transformed L. reuteri culture were 0.68 and 0.42 U/mL, respectively. Transformed L. reuteri improved the feed conversion ratio of broilers from 21 to 42 days of age and over the whole feeding period. However, there was no effect on body weight gain and feed intake of chicks. Transformed L. reuteri supplementation improved levels of ash, calcium and phosphorus in tibiae at day 21 and of phosphorus at day 42. In addition, populations of Escherichia coli, Veillonella spp. and Bacteroides vulgatus were decreased, while populations of Bifidobacterium genus and Lactobacillus spp. were increased in the cecum at day 21. PMID:25050780

Wang, Lei; Yang, Yuxin; Cai, Bei; Cao, Pinghua; Yang, Mingming; Chen, Yulin

2014-01-01

410

Effect of Lactobacillus sakei supplementation in children with atopic eczema–dermatitis syndrome  

Microsoft Academic Search

Background: Probiotics have been suggested to be useful in children with atopic eczema-dermatitis syndrome (AEDS). Objective: To assess the clinical effect of Lactobacillus sakei supplementation in children with AEDS. Methods: In a double-blind, placebo-controlled trial, children aged 2 to 10 years with AEDS with a minimum SCORing of Atopic Dermatitis (SCORAD) score of 25 were randomized to receive either daily

Sung-Il Woo; Ji-Yoon Kim; Yong-Ju Lee; Nam-Shik Kim; Youn-Soo Hahn

2010-01-01

411

Effects of Lactobacillus casei and Bifidobacterium breve on urinary oxalate excretion in nephrolithiasis patients  

Microsoft Academic Search

It had been suggested that lactic acid bacteria (LAB) may degrade oxalate in the intestinal lumen, reducing urinary oxalate\\u000a excretion. We aimed to evaluate the effect of a LAB mixture containing Lactobacillus casei (LC) and Bifidobacterium breve (BB) (LC + BB) upon urinary oxalate reduction in stone-forming (SF) patients without hyperoxaluria under conditions of an\\u000a oxalate-rich diet. After an oxalate restriction period

Renato Ribeiro Nogueira Ferraz; Natália Cristina Marques; Leila Froeder; Viviane Barcellos Menon; Priscila Reina Siliano; Alessandra Calábria Baxmann; Ita Pfeferman Heilberg

2009-01-01

412

Identification of the transcriptional response of human intestinal mucosa to Lactobacillus plantarum WCFS1 in vivo  

Microsoft Academic Search

BACKGROUND: There is limited knowledge on the extent and dynamics of the mucosal response to commensal and probiotic species in the human intestinal lumen. This study aimed to identify the acute, time-dependent responses of intestinal mucosa to commensal Lactobacillus plantarum WCFS1 in vivo in two placebo-controlled human intervention studies in healthy volunteers. Transcriptional changes in duodenal mucosa upon continuous intraduodenal

Freddy J Troost; Peter van Baarlen; Patrick Lindsey; Andrea Kodde; Willem M de Vos; Michiel Kleerebezem; Robert-Jan M Brummer

2008-01-01

413

Purification and Characterization of a Feruloyl Esterase from the Intestinal Bacterium Lactobacillus acidophilus  

Microsoft Academic Search

Dietary ferulic acid (FA), a significant antioxidant substance, is currently the subject of extensive research. FA in cereals exists mainly as feruloylated sugar ester. To release FA from food matrices, it is necessary to cleave ester cross-linking by feruloyl esterase (FAE) (hydroxycinnamoyl esterase; EC 3.1.1.73). In the present study, the FAE from a human typical intestinal bacterium, Lactobacillus acidophilus, was

Xiaokun Wang; Xin Geng; Yukari Egashira; Hiroo Sanada

2004-01-01

414

Unusual Organization for Lactose and Galactose Gene Clusters in Lactobacillus helveticus  

Microsoft Academic Search

The nucleotide sequences of the Lactobacillus helveticus lactose utilization genes were determined, and these genes were located and oriented relative to one another. The lacLM genes (encoding the -galactosidase protein) were in a divergent orientation compared to lacR (regulatory gene) and lacS (lactose transporter). Downstream from lacM was an open reading frame (galE) encoding a UDP-galactose 4 epimerase, and the

Maria Grazia Fortina; Giovanni Ricci; Diego Mora; Simone Guglielmetti; Pier Luigi Manachini

2003-01-01

415

Crystal Structure of ?-Galactosidase from Lactobacillus acidophilus NCFM: Insight into Tetramer Formation and Substrate Binding  

Microsoft Academic Search

Lactobacillus acidophilus NCFM is a probiotic bacterium known for its beneficial effects on human health. The importance of ?-galactosidases (?-Gals) for growth of probiotic organisms on oligosaccharides of the raffinose family present in many foods is increasingly recognized. Here, the crystal structure of ?-Gal from L. acidophilus NCFM (LaMel36A) of glycoside hydrolase (GH) family 36 (GH36) is determined by single-wavelength

Folmer Fredslund; Maher Abou Hachem; René Jonsgaard Larsen; Pernille Gerd Sørensen; Pedro M. Coutinho; Leila Lo Leggio; Birte Svensson

2011-01-01

416

Bile Salt Hydrolase Activity of Three Strains of Lactobacillus acidophilus1  

Microsoft Academic Search

Three strains of Lactobacillus acidophilus, two from human intestinal origin (016 and L1) and one from porcine intestinal origin (ATCC 43121), were tested for their bile salt deconjugation activity. The L. acidophilus ATCC 43121 had more deconjugating ac- tivity of both sodium glycocholate and sodium taurocholate at pH 6.5 than did either L. acidophilus 016 or L1. The activity of

G. Corzo; S. E. Gilliland

1999-01-01

417

Dynamic cell surface hydrophobicity of Lactobacillus strains with and without surface layer proteins  

Microsoft Academic Search

Variations in surface hydrophobicity of six Lactobacillus strains with and without an S-layer upon changes in ionic strength are derived from contact angle measurements with low- and high-ionic-strength aqueous solutions. Cell surface hydrophobicity changed in response to changes in ionic strength in three out of the six strains, offering these strains a versatile mechanism to adhere to different surfaces. The

V. Vadillo-Rodriguez; Henk J. Busscher; Willem Norde; Joop de Vries; Mei van der H. C

2004-01-01

418

Purification and characterization of a cell surface-associated esterase from Lactobacillus fermentum DT41  

Microsoft Academic Search

A cell surface-associated esterase from Lactobacillus fermentum DT41, a starter used to produce Parmesan cheese, was purified to homogeneity by chromatography on Q-Sepharose, Sephacryl 200, Phenyl-Superose and Mono Q. The enzyme, together with a distinct cytoplasmic esterase, expressed the highest activity during the exponential phase of growth. The esterase was a monomer with a Mr of ca 67 kDa and

Marco Gobbetti; Emanuele Smacchi; Aldo Corsetti

1997-01-01

419

L(+)-lactic acid production using Lactobacillus casei in solid-state fermentation.  

PubMed

Lactobacillus casei was grown at 37 degrees C on sugarcane bagasse (5 g) soaked with cassava starch hydrolysate (final moistening volume 34 ml) containing 3 g reducing sugar in a solid-state condition. The maximum yield of L-lactic acid after various process optimisations was 2.9 g/5 g initial substrate corresponding to 97% conversion of sugar to lactic acid with initial substrate moisture of 72%. PMID:16247675

Rojan, Pappy John; Nampoothiri, Kesavan Madhavan; Nair, Athira Syamaprasad; Pandey, Ashok

2005-11-01

420

Cloning, sequence analysis, and expression of Lactobacillus casei phage PL1 lysis genes  

Microsoft Academic Search

Summary.  ?The genes encoding the host cell wall-lytic proteins were searched in the genome DNA of phage PL-1 active against Lactobacillus casei ATCC 27092 by comparing the amino acid sequences with those of others using a computer software of the DDBJ data base. The\\u000a gene regions found were cloned into E. coli by inserting PCR-amplified DNA fragments into the EcoRI site

N. Kashige; Y. Nakashima; F. Miake; K. Watanabe

2000-01-01

421

Thioredoxin reductase is a key factor in the oxidative stress response of Lactobacillus plantarum WCFS1  

Microsoft Academic Search

BACKGROUND: Thioredoxin (TRX) is a powerful disulfide oxido-reductase that catalyzes a wide spectrum of redox reactions in the cell. The aim of this study is to elucidate the role of the TRX system in the oxidative stress response in Lactobacillus plantarum WCFS1. RESULTS: We have identified the trxB1-encoded thioredoxin reductase (TR) as a key enzyme in the oxidative stress response

L. Mariela Serrano; Douwe Molenaar; Michiel Wels; Bas Teusink; Peter A. Bron; Willem M. de Vos; Eddy J. Smid

2007-01-01

422

Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum  

Microsoft Academic Search

BACKGROUND: A reliable quenching and metabolite extraction method has been developed for Lactobacillus plantarum. The energy charge value was used as a critical indicator for fixation of metabolism. RESULTS: Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. Only the solutions containing either 70 mM HEPES or 0.85% (w\\/v) ammonium carbonate (pH 5.5) caused

Magda Faijes; Astrid E Mars; Eddy J Smid

2007-01-01

423

Methodology employing lactobacillus GG for reduction of plasma endotoxin levels circulating in-vivo  

US Patent & Trademark Office Database

A method is provided for reducing the quantity of endotoxin circulating in the blood plasma of a living subject affected by an endotoxin-mediated clinical state. The method employs a formulated preparation comprising at least one persistent, acid-resistant, and colonizing Lactobacillus species in combination with a biocompatible carrier. The user then introduces the formulated preparation to the gastrointestinal tract of the subject on one or on multiple occasions.

1995-05-09

424

Antibiotic resistance of potential probiotic bacteria of the genus Lactobacillus from human gastrointestinal microbiome  

Microsoft Academic Search

Thirteen Lactobacillus strains isolated from the gastrointestinal microbiome of people from the territory of the former Soviet Union have been studied\\u000a for resistance to 15 antibiotics of different nature, namely, penicillins, aminoglycosides, macrolides, lincosamides, tetracyclines,\\u000a chloramphenicol, and rifampicin. The strains included four strains of L. plantarum, four of L. helveticus, three of L. casei\\/paracasei, one of L. rhamnosus, and one

S. G. Botina; E. U. Poluektova; A. A. Glazova; N. V. Zakharevich; N. V. Koroban; V. V. Zinchenko; M. M. Babykin; O. G. Zhilenkova; A. M. Amerkhanova; V. N. Danilenko

2011-01-01

425

Kinetic model for the inactivation of Lactobacillus plantarum by pulsed electric fields  

Microsoft Academic Search

The kinetics of Lactobacillus plantarum inactivation by pulsed electric fields (PEF) was studied in two different growth stages (exponential and stationary), but in the same reference medium (0.6% peptone water). Electric field intensity and treatment time varied from 20 to 28 kV\\/cm and 30 to 240 ?s, respectively. The experimental data showed that cells in the exponential growth stage were

D Rodrigo; P Ru??z; G. V Barbosa-Cánovas; A Mart??nez; M Rodrigo

2003-01-01

426

Inactivation of Lactobacillus brevis in orange juice by high-intensity pulsed electric fields  

Microsoft Academic Search

High-intensity pulsed electric fields (HIPEF) is a non-thermal preservation method which is believed to be able to inactivate spoilage micro-organisms such as Lactobacillus brevis. The effects of HIPEF parameters (electric field strength, treatment time, pulse polarity, frequency and pulse width) and heat pasteurization (90°C\\/1min) were evaluated on samples of orange juice inoculated with L. brevis (108cfu\\/ml). HIPEF as well as

Pedro Elez-Martínez; Joan Escolà-Hernández; Robert C. Soliva-Fortuny; Olga Martín-Belloso

2005-01-01

427

Lactobacillus priming of the respiratory tract: heterologous immunity and protection against lethal pneumovirus infection  

PubMed Central

We showed previously that wild-type mice primed via intranasal inoculation with live or heat-inactivated Lactobacillus species were fully (100%) protected against the lethal sequelae of infection with the virulent pathogen, pneumonia virus of mice (PVM), a response that is associated with diminished expression of proinflammatory cytokines and diminished virus recovery. We show here that 40% of the mice primed with live Lactobacillus survived when PVM challenge was delayed for 5 months. This robust and sustained resistance to PVM infection resulting from prior interaction with an otherwise unrelated microbe is a profound example of heterologous immunity. We undertook the present study in order to understand the nature and unique features of this response. We found that intranasal inoculation with L. reuteri elicited rapid, transient neutrophil recruitment in association with proinflammatory mediators (CXCL1, CCL3, CCL2, CXCL10, TNF-alpha and IL-17A) but not Th1 cytokines. IFN? does not contribute to survival promoted by Lactobacillus-priming. Live L. reuteri detected in lung tissue underwent rapid clearance, and was undetectable at 24 hrs after inoculation. In contrast, L. reuteri peptidoglycan (PGN) and L. reuteri genomic DNA (gDNA) were detected at 24 and 48 hours after inoculation, respectively. In contrast to live bacteria, intranasal inoculation with isolated L. reuteri gDNA elicited no neutrophil recruitment, had minimal impact on virus recovery and virus-associated production of CCL3, and provided no protection against the negative sequelae of virus infection. Isolated PGN elicited neutrophil recruitment and proinflammatory cytokines but did not promote sustained survival in response to subsequent PVM infection. Overall, further evaluation of the responses leading to Lactobacillus-mediated heterologous immunity may provide insight into novel antiviral preventive modalities.

Garcia-Crespo, Katia E.; Chan, Calvin C.; Gabryszewski, Stanislaw J.; Percopo, Caroline M.; Rigaux, Peter; Dyer, Kimberly D.; Domachowske, Joseph B.; Rosenberg, Helene F.

2013-01-01

428

Lactobacillus paracasei NFBC 338 producing recombinant beta-glucan positively influences the functional properties of yoghurt  

Microsoft Academic Search

The performance of a recombinant probiotic strain Lactobacillus paracasei NFBC 338, which contains the Pediococcus parvulus glycosyltransferase gene (responsible for beta-glucan production), when used as an adjunct in yoghurt manufacture was evaluated. In situ production of beta-glucan during yoghurt manufacture and storage caused a significant decrease in syneresis, confirming the water-binding ability of beta-glucan. Yoghurt textural characteristics were significantly improved

Niamh Kearney; Helena M. Stack; John T. Tobin; Valérie Chaurin; Mark A. Fenelon; Gerald F. Fitzgerald; R. Paul Ross; Catherine Stanton

2011-01-01

429

Progress in table olive debittering: Degradation in vitro of oleuropein and its derivatives by Lactobacillus plantarum  

Microsoft Academic Search

Oleuropein, a bitter-tasting secoiridoid glycoside present in olive leaves and fruit (Olea europaea L.), is hydrolyzed by oleuropeinolyticLactobacillus plantarum strains. The work reports the results of a gas-chromatographic study of the oleuropein derivatives released by incubation\\u000a withL. plantarum B21, isolated from table olive brines, and byL. plantarum ATCC 8014. Process kinetics indicate that the bacterial strains initially hydrolyze the oleuropein

V. Marsilio; B. Lanza; N. Pozzi

1996-01-01

430

Effect of oleuropein and sodium chloride on viability and metabolism of Lactobacillus plantarum  

Microsoft Academic Search

The effect of the addition of oleuropein (OLP) and NaCl on the growth and the DL-lactic acid production of Lactobacillus plantarum DSM 10492 has been investigated by using an unconventional medium. The growth of L. plantarum was not inhibited by the addition of increasing amounts of untreated OLP in the presence or absence of glucose. However,\\u000a bacterial cells grew in

N. Rozés; C. Peres

1996-01-01

431

Rapid and Simple Method for the Encapsulation of Lactobacillus reuteri in the Production of Lactic Acid  

Microsoft Academic Search

\\u000a Lactic acid (LA) is the most widely used multifunctional organic acid and approximately 50% of it is produced by biotechnological\\u000a process. Immobilizing microbial cells not only improves cell retention but also protects cells from harsh environmental conditions\\u000a during LA production. The objectives of this research were therefore to develop a simple method to encapsulate (immobilize)\\u000a Lactobacillus reuteri for the continuous

Salam A. Ibrahim; Somphavanh Phetsomphou; Chyer Kim; Abolghasem Shahbazi; Chung W. Seo; Amer AbuGhazaleh; Moussa M. E. Salem

432

An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions  

Microsoft Academic Search

An improved method of microencapsulation was developed to increase the efficacy of capsules in protecting the encapsulated bacteria under simulated gastric conditions. Lactobacillus acidophilus CSCC 2400 was encapsulated in calcium alginate and tested for its survival in simulated gastric conditions. The effects of different capsule sizes (200, 450, 1000 ?m), different sodium alginate concentrations (0.75%, 1%, 1.5%, 1.8% and 2%

V. Chandramouli; K. Kailasapathy; P. Peiris; M. Jones

2004-01-01

433

PepR1, a CcpA-like transcription regulator of Lactobacillus delbrueckii subsp. lactis  

Microsoft Academic Search

The PepR1 protein from Lactobacillus delbrueckii subsp. lactis DSM 7290 shares extensive homology with catabolite-control proteins from various Gram- positive bacteria. Expression of the subcloned pepR1 gene allowed for partial complementation of a ccpA defect in Staphylococcus xylosus. The influence of PepR1 on transcription of the prolidase gene pepQ, which is located adjacent to pepR1, was examined by use of

Joachim Schick; Beate Weber; R. Klein; Bernhard Henrich; Fachbereich Biologie

434

Immunogenicity and protective efficacy of orally or intranasally administered recombinant Lactobacillus casei expressing ETEC K99  

Microsoft Academic Search

In an effort to develop a safe and effective vaccine for the prevention of enterotoxigenic Escherichia coli (ETEC) K99 infections, we have developed a surface antigen display system using pgsA (poly-?-glutamate synthetase A) as an anchoring matrix. The recombinant fusion proteins comprised of pgsA and fimbriae protein of ETEC K99 were stably expressed on Lactobacillus casei. Surface localization of the

Chun-Hua Wei; Jian-Kui Liu; Xi-Lin Hou; Li-Yun Yu; Jong-Soo Lee; Chul-Joong Kim

2010-01-01

435

Diagnostic difficulties of Lactobacillus casei bacteraemia in immunocompetent patients: A case report  

Microsoft Academic Search

INTRODUCTION: Lactobacilli are currently proposed as probiotic agents in several dietary products. In blood cultures, they are usually considered as contaminants, but in recent years they have been recognized as causal infectious agents of endocarditis, urinary tract infections, meningitis, intra-abdominal infections and bacteraemia. CASE PRESENTATION: We report a case of Lactobacillus casei bacteraemia in a 66-year-old immunocompetent man with a

Chiara Tommasi; Francesco Equitani; Marcello Masala; Milva Ballardini; Marco Favaro; Marcello Meledandri; Carla Fontana; Pasquale Narciso; Emanuele Nicastri

2008-01-01

436

Surface Display of Foreign Epitopes on the Lactobacillus brevis S-Layer  

PubMed Central

So far, the inability to establish viable Lactobacillus surface layer (S-layer) null mutants has hampered the biotechnological applications of Lactobacillus S-layers. In this study, we demonstrate the utilization of Lactobacillus brevis S-layer subunits (SlpA) for the surface display of foreign antigenic epitopes. With an inducible expression system, L. brevis strains producing chimeric S-layers were obtained after testing of four insertion sites in the slpA gene for poliovirus epitope VP1, that comprises 10 amino acids. The epitope insertion site allowing the best surface expression was used for the construction of an integration vector carrying the gene region encoding the c-Myc epitopes from the human c-myc proto-oncogene, which is composed of 11 amino acids. A gene replacement system was optimized for L. brevis and used for the replacement of the wild-type slpA gene with the slpA-c-myc construct. A uniform S-layer, displaying on its surface the desired antigen in all of the S-layer protein subunits, was obtained. The success of the gene replacement and expression of the uniform SlpA-c-Myc recombinant S-layer was confirmed by PCR, Southern blotting MALDI-TOF mass spectrometry, whole-cell enzyme-linked immunosorbent assay, and immunofluorescence microscopy. Furthermore, the integrity of the recombinant S-layer was studied by electron microscopy, which indicated that the S-layer lattice structure was not affected by the presence of c-Myc epitopes. To our knowledge, this is the first successful expression of foreign epitopes in every S-layer subunit of a Lactobacillus S-layer while still maintaining the S-layer lattice structure.

Avall-Jaaskelainen, Silja; Kyla-Nikkila, Kari; Kahala, Minna; Miikkulainen-Lahti, Terhi; Palva, Airi

2002-01-01

437

Changes in steady state on introduction of a Lactobacillus contaminant to a continuous culture ethanol fermentation  

Microsoft Academic Search

Lactobacillus paracasei   was introduced as a contaminant into a multistage continuous culture ethanol fermentation system at ratios of 1:100, 1:1,\\u000a and 70:1 with Saccharomyces cerevisiae, but failed to overtake the yeast. None of the inoculation ratios allowed L. paracasei to affect S. cerevisiae in the first fermentor in the multistage system. S. cerevisiae remained constant at ?3?107 CFU\\/ml regardless of

D Bayrock; W M Ingledew

2001-01-01

438

Selection of Vaginal H 2 O 2 Generating Lactobacillus Species for Probiotic Use  

Microsoft Academic Search

.   Lactobacilli are believed to contribute to the control of the vaginal microflora by different mechanisms such as production\\u000a of antagonistic substances like lactic acid, bacteriocins, and H2O2. This paper describes the selection of H2O2-generating lactobacilli among 35 hydrophobic isolates from the human vagina. Lactobacillus crispatus F117, which generated the highest H2O2 level, was chosen to study: (a) the kinetics

Virginia S. Ocaña; Aída A. Pesce de Ruiz Holgado; María E. Nader-Macías

1999-01-01

439

Synergistic antidigestion effect of Lactobacillus rhamnosus and bovine colostrums in simulated gastrointestinal tract (in vitro)  

Microsoft Academic Search

Probiotics and bovine colostrums had been proven to be beneficial for human health. Lactobacillus rhamnosus ZDY114 and anti-Helicobacter pylori bovine colostrums were used for the preparation of microecological additives, and their synergistic antidigestion effect\\u000a in the simulated gastrointestinal tract (in vitro) was investigated. Either L. rhamnosus or purified IgG from immune colostrums was very sensitive in simulated gastric environment and

Wei Hua; Xu Yang; Xiong Yonghua; Xu Feng; Liu Gengpin

2007-01-01

440

Isolation and characterisation of a ropy Lactobacillus strain producing the exopolysaccharide kefiran  

Microsoft Academic Search

A capsular-polysaccharide-producing strain, LM-17, was isolated from kefir grains and was identified as a slime-forming,\\u000a rod-shaped Lactobacillus. According to 1H- and 13C-NMR spectral data, the exopolysaccharide produced by the isolated bacterial strain is identical to the glucogalactan extracted\\u000a from kefir grains and therefore known as kefiran. The kefiran produced was characterised by means of viscosity, optical rotatory\\u000a power, circular dichroism

L. Micheli; D. Uccelletti; C. Palleschi; V. Crescenzi

1999-01-01

441

Lactic acid fermentation by cells of Lactobacillus rhamnosus immobilizedin polyacrylamide gel  

Microsoft Academic Search

Summary  The process of lactic acid fermentation of lactose to lactic acid by Lactobacillus rhamnosus ATCC 7469 has been studied. The following processes have been explored: growth kinetics, as well as lactose utilization,\\u000a production of lactic acid and further degradation of lactic acid. The immobilization experiments were conducted with microbial\\u000a cells entrapped in polyacrylamide gels. Gels with different ratios of the

Kaloyan K. Petrov; Dragomir S. Yankov; Venko N. Beschkov

2006-01-01

442

Survival of Lactobacillus acidophilus and Bifidobacterium bifidum in commercial yoghurt during refrigerated storage  

Microsoft Academic Search

Samples of five brands of commercial yoghurt were obtained directly from the processors and enumeration of viable Lactobacillus acidophilus and Bifidobacterium bifidum and determination of pH was carried out at three-day intervals over a five-week period. Three of the five products contained 107–108 g?1 viable cells of L. acidophilus, whereas the other two products contained ? 105 of this organism

Nagendra P. Shah; Warnakulasuriya E. V. Lankaputhra; Margaret L. Britz; William S. A. Kyle

1995-01-01

443

Purification and DNA-binding properties of the integrase protein Int encoded by Lactobacillus plantarum phage  

Microsoft Academic Search

The Lactobacillus plantarum temperate phage ?g1e (42?259bp) encodes an integrase gene int linked to a phage attachment site attP (Kakikawa et al., 1997). To investigate ?g1e recombination, the integrase protein Int was overproduced in Escherichia coli under the T7 promoter, and purified. The Int protein had an apparent molecular mass of 42.0kDa, corresponding well with that (45.5kDa) predicted from the

Hiroo Yasukawa; Makiko Kakikawa; Yukito Masamune; Akira Taketo; Ken-Ichi Kodaira

1997-01-01

444

Lactobacillus plantarum 299v Prevents Caspase-Dependent Apoptosis In Vitro  

Microsoft Academic Search

Selective microbes used as probiotics can enhance epithelial cell protection. We have previously shown that a Lactobacillus\\u000a plantarum strain 299v (Lp299v) has the ability to induce mucin genes. In the current study, we utilized a cytokine model of inflammation\\u000a in cell culture to study the modulation of apoptosis by this probiotic. HT-29 cells were pre-incubated with the Lp299v or\\u000a L.

Natalie S. Dykstra; Lucie Hyde; Alexander MacKenzie; David R. Mack

2011-01-01

445

Probiotic Lactobacillus spp. Diminish Helicobacter hepaticus-Induced Inflammatory Bowel Disease in Interleukin10Deficient Mice  

Microsoft Academic Search

Clinical and experimental evidence has demonstrated the potential role of probiotics in the prevention or treatment of inflammatory bowel disease. Probiotic clones with direct immunomodulatory activity may have anti-inflammatory effects in the intestine. We investigated the roles of tumor necrosis factor alpha (TNF-)- inhibitory Lactobacillus clones with a pathogen-induced murine colitis model. Murine-derived probiotic lacto- bacilli were selected in vitro

Jeremy A. Pena; Arlin B. Rogers; Zhongming Ge; Vivian Ng; Sandra Y. Li; James G. Fox; James Versalovic

2005-01-01

446

Characterization of the polysaccharides from a Lactobacillus brevis and from sugary kefir grains  

Microsoft Academic Search

Summary The gel-forming polysaccharide of the sugary kefir grains (11.5% of dry matter) or one taken from aLactobacillus brevis culture were identified as dextrans with some 1)-Gp-(3 links in the main chain, with a ratio (branched\\/total units) of 0.19 and 0.14 respectively, instead of 0.07 for the non-gelling polysaccharide.

M. Pidoux; J. M. Brillouet; B. Quemener

1988-01-01

447

Two 2[5H]-Furanones as Possible Signaling Molecules in Lactobacillus helveticus  

PubMed Central

Two 2[5H]-furanones, in association with medium-chain fatty acids, were released in whey by Lactobacillus helveticus exposed to oxidative and heat stresses. This species plays an important role in cheese technology, particularly for Swiss-type cheeses and Grana cheese. Moreover, it significantly contributes to cheese ripening by means of an early autolysis and the release of enzymes during processing. Experimental evidence of the involvement of the two 2[5H]-furanones, detected by a gas chromatography-mass spectrometry/solid-phase microextraction technique, in the autolysis phenomenon has been obtained. Zymograms performed by using renaturing sodium dodecyl sulfate-polyacrylamide gels were used to detect the bioactivity of the supernatants containing the two furanones on fresh cells of the same strain. In addition to bands corresponding to known autolysins, new autolysins were detected concomitant with the exposure of Lactobacillus helveticus to the supernatants, which can be regarded as conditioned media (CM), and to a commercial furanone, 5-ethyl-3-hydroxy-4-methyl-2[5H]-furanone (HEMFi), having spectral data similar to those of the newly described 2[5H]-furanones. Morphological changes were observed when fresh cells were exposed to CM containing the two 2[5H]-furanones and HEMFi. The two furanones produced by Lactobacillus helveticus, which met a number of criteria to be included in cell-cell signaling molecules, have a presumptive molecular mass lower than those of already known 3[2H]-furanones having an autolytic activity and being produced by gram-negative bacteria. Moreover, they present a different chemical structure with respect to the furanones already identified as products of Lactococcus lactis subsp. cremoris or to those identified in some cheeses with Lactobacillus helveticus as a starter culture.

Ndagijimana, Maurice; Vallicelli, Melania; Cocconcelli, P. Sandro; Cappa, Fabrizio; Patrignani, Francesca; Lanciotti, Rosalba; Guerzoni, M. Elisabetta

2006-01-01

448

Multicenter, randomized, controlled trial of heat-killed Lactobacillus acidophilus LB in patients with chronic diarrhea  

Microsoft Academic Search

Chronic diarrhea is a common bowel disorder; disturbance of intestinal microorganisms may play a role in its pathogenesis.\\u000a This study assessed the clinical efficacy of lyophilized, heat-killedLactobacillus acidophilus LB versus living lactobacilli in the treatment of chronic diarrhea. One hundred thirty-seven patients with chronic diarrhea\\u000a were randomly allocated to receive either a 4-week course of 2 capsules of Lacteol® Fort

Shy-Dong Xiao; Zhong De Zhang; Hong Lu; Shi Hu Jiang; Hou Yu Liu; Geng Sheng Wang; Guo Ming Xu; Zhong Bing Zhang; Geng Jin Lin; Guo Liang Wang

2003-01-01

449

Glutathione Protects Lactobacillus sanfranciscensis against Freeze-Thawing, Freeze-Drying, and Cold Treatment  

Microsoft Academic Search

Lactobacillus sanfranciscensis DSM20451 cells containing glutathione (GSH) displayed significantly higher resistance against cold stress induced by freeze-drying, freeze-thawing, and 4°C cold treatment than those without GSH. Cells containing GSH were capable of maintaining their membrane structure intact when exposed to freeze-thawing. In addition, cells containing GSH showed a higher proportion of unsaturated fatty acids in cell membranes upon long-term cold

Juan Zhang; Guo-Cheng Du; Yanping Zhang; Xian-Yan Liao; Miao Wang; Yin Li; Jian Chen

2010-01-01

450

Lactobacillus plantarum DSM 2648 is a potential probiotic that enhances intestinal barrier function.  

PubMed

The aim of this research was to identify bacterial isolates having the potential to improve intestinal barrier function. Lactobacillus plantarum strains and human oral isolates were screened for their ability to enhance tight junction integrity as measured by the transepithelial electrical resistance (TEER) assay. Eight commercially used probiotics were compared to determine which had the greatest positive effect on TEER, and the best-performing probiotic strain, Lactobacillus rhamnosus HN001, was used as a benchmark to evaluate the isolates. One isolate, L. plantarum DSM 2648, was selected for further study because it increased TEER 135% more than L. rhamnosus HN001. The ability of L. plantarum DSM 2648 to tolerate gastrointestinal conditions and adhere to intestinal cells was determined, and L. plantarum DSM 2648 performed better than L. rhamnosus HN001 in all the assays. Lactobacillus plantarum DSM 2648 was able to reduce the negative effect of Escherichia coli [enteropathogenic E. coli (EPEC)] O127:H6 (E2348/69) on TEER and adherence by as much as 98.75% and 80.18%, respectively, during simultaneous or prior coculture compared with EPEC incubation alone. As yet, the precise mechanism associated with the positive effects exerted by L. plantarum DSM 2648 are unknown, and may influence its use to improve human health and wellness. PMID:20618863

Anderson, Rachel C; Cookson, Adrian L; McNabb, Warren C; Kelly, William J; Roy, Nicole C

2010-08-01

451

Preliminary Evaluation of Probiotic Properties of Lactobacillus Strains Isolated from Sardinian Dairy Products  

PubMed Central

Twenty-three Lactobacillus strains of dairy origin were evaluated for some functional properties relevant to their use as probiotics. A preliminary subtractive screening based on the abilities to inhibit the growth of microbial pathogens and hydrolyze conjugated bile salts was applied, and six strains were selected for further characterization including survival under gastrointestinal environmental conditions, adhesion to gut epithelial tissue, enzymatic activity, and some safety properties. All selected strains maintained elevated cell numbers under conditions simulating passage through the human gastrointestinal tract, well comparable to the values obtained for the probiotic strain Lactobacillus rhamnosus GG, and were able to adhere to Caco-2 cells to various extents (from 3 to 20%). All strains exhibited high aminopeptidase, and absent or very low proteolytic and strong ?-galactosidase activities; none was found to be haemolytic or to produce biogenic amines and all were susceptible to tetracycline, chloramphenicol, erythromycin, ampicillin, and amoxicillin/clavulanic acid. Our results indicate that the Lactobacillus strains analyzed could be considered appropriate probiotic candidates, due to resistance to GIT simulated conditions, antimicrobial activity, adhesion to Caco-2 cell-line, and absence of undesirable properties. They could be used as adjunct cultures for contributing to the quality and health related functional properties of dairy products.

Pisano, Maria Barbara; Viale, Silvia; Conti, Stefania; Deplano, Maura; Melis, Maria Paola; Deiana, Monica

2014-01-01

452

Probiotic potential of Lactobacillus spp. isolated from Brazilian regional ovine cheese.  

PubMed

Twelve Lactobacillus isolates from Brazilian starter-free ovine cheeses were evaluated for their probiotic potential. The strains were identified by 16S rDNA sequencing as Lactobacillus plantarum (7), Lb. brevis (2), Lb. casei (2) and Lb. parabuchneri (1). All strains showed variable resistance to gastric juices and relative tolerance to pancreatin and bile salts. Only five strains of Lb. plantarum could not deconjugate the sodium salt of taurodeoxycholic acid. Autoaggregation ability after 24 h was above 50% and hydrophobicity was higher than 60% for most strains. All lactobacilli could inhibit linolenic acid oxidation, except Lb. parabuchneri strain, whereas none of them could scavenge DPPH radical. ?-Galactosidase activity ranged from 47·7 to 2503 Miller units. Inhibition of food pathogens Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella typhimurium was demonstrated and the production of organic acids could be associated with this effect. The Lactobacillus strains from Brazilian regional ovine cheese showed interesting functional characteristics, mainly the strains Lb. brevis SM-B and Lb. plantarum SM-I. Both presented high acid tolerance. In addition, Lb. brevis SM-B also displayed remarkable antioxidant activity and Lb. plantarum SM-I was the highest ?-galactosidase producer, exhibited high autoaggregation and hydrophobicity properties. PMID:23171587

Meira, Stela Maris Meister; Helfer, Virginia Etges; Velho, Renata Voltolini; Lopes, Fernanda Cortez; Brandelli, Adriano

2012-02-01

453

Chromosomal-gene-mediated inhibition of intestinal and foodborne pathogens by Lactobacillus acidophilus AA11.  

PubMed

Approximately 63 strains of Lactobacillus acidophilus were isolated from Egyptian home-made cheese and examined for production of antagonism. Only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. Staphylococcus aureus and Bacillus cereus) and pathogens (i.e. E. coli, Salmonella sp. and Shigella sp.). Lactobacillus acidophilus AA11 produced a more antimicrobial activity with a wide range of inhibition. The agent AA11 was sensitive to proteolytic enzymes and retained full activity after 30 min at 100 degrees C. Activity against sensitive cells was bactericidal but not bacteriolytic. The compound was produced during growth phase and can be extracted from the culture supernatant fluids with n-Butanol. 12 % SDS-PAGE analysis of 40% ammonium sulphate precipitated agent showed two peptides with molecular weights of approximately 36 kDa and approximately 29 kDa. No plasmid was identified in Lactobacillus acidophilus AA11 indicating that the genes encoding the inhibitory agent located on the chromosome. These characteristics identify the inhibitory substance as a bacteriocin, designated acidocin AA11 and confer the agent an application potential as a biopreservative. PMID:17357571

Abo-Amer, Aly E

2006-01-01

454

Molecular characterization of antimicrobial compound produced by Lactobacillus acidophilus AA11.  

PubMed

Approximately 63 strains of Lactobacillus acidophilus were isolated from Egyptian home-made cheese and examined for production of antagonism. Only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. Staphylococcus aureus and Bacillus cereus) and pathogens (i.e. E. coli, Salmonella sp. and Shigella sp.). Lactobacillus acidophilus AA11 produced higher antimicrobial activity with a wide range of inhibition. The agent AA11 was sensitive to proteolytic enzymes and retained full activity after 30 min at 100 degrees C. Activity against sensitive cells was bactericidal but not bacteriolytic. The compound was produced during growth phase and could be extracted from the culture supernatant fluids with n-butanol. 12% SDS-PAGE analysis of 40% ammonium sulphate precipitated agent showed two peptides with molecular weights of approximately 36 kDa and approximately 29 kDa. No plasmid was identified in Lactobacillus acidophilus AA11 indicating that the genes encoding the inhibitory agent were located on the chromosome. These characteristics identify the inhibitory substance as a bacteriocin, designated acidocin AA11 and confer the agent an application potential as a biopreservative. PMID:17899791

Abo-Amer, Aly E

2007-06-01

455

Characterization of the Genome of the Dairy Lactobacillus helveticus Bacteriophage ?AQ113  

PubMed Central

The complete genomic sequence of the dairy Lactobacillus helveticus bacteriophage ?AQ113 was determined. Phage ?AQ113 is a Myoviridae bacteriophage with an isometric capsid and a contractile tail. The final assembled consensus sequence revealed a linear, circularly permuted, double-stranded DNA genome with a size of 36,566 bp and a G+C content of 37%. Fifty-six open reading frames (ORFs) were predicted, and a putative function was assigned to approximately 90% of them. The ?AQ113 genome shows functionally related genes clustered together in a genome structure composed of modules for DNA replication/regulation, DNA packaging, head and tail morphogenesis, cell lysis, and lysogeny. The identification of genes involved in the establishment of lysogeny indicates that it may have originated as a temperate phage, even if it was isolated from natural cheese whey starters as a virulent phage, because it is able to propagate in a sensitive host strain. Additionally, we discovered that the ?AQ113 phage genome is closely related to Lactobacillus gasseri phage KC5a and Lactobacillus johnsonii phage Lj771 genomes. The phylogenetic similarities between L. helveticus phage ?AQ113 and two phages that belong to gut species confirm a possible common ancestral origin and support the increasing consideration of L. helveticus as a health-promoting organism.

Scaltriti, Erika; Rossetti, Lia; Guffanti, Alessandro; Armiento, Angelarita; Fornasari, Maria Emanuela; Grolli, Stefano; Carminati, Domenico; Brini, Elena; Pavan, Paolo; Felsani, Armando; D'Urzo, Annalisa; Moles, Anna; Claude, Jean-Baptiste; Grandori, Rita; Ramoni, Roberto; Giraffa, Giorgio

2013-01-01

456

Application of Lactobacillus immobilized by Activated Carbon Fiber in Fermentation of Lactic Acid in Starch Wastewater  

NASA Astrophysics Data System (ADS)

Activated carbon fibers (ACF) as the carrier of Lactobacillus was introduced into fermenting system, and a method of modifying the surface of ACF by HNO3-Fe (III) was established. Factors that affect ACF carrier's effect on immobilization of Lactobacillus were studied. HCl, H2SO4, HNO3 and FeCl3 solutions were respectively used to modify the surface properties of ACF. The amount of Fe (III) carried on ACF surface was 0.1563 mol/kg after ACF surface was modified by HNO3 for 5 h and then by 0.1 mol/L FeCl3 for 4 h, when the thickness of Lactobacillus on a single silk of carrier reached 40 ?m. When ACF modified by HNO3-Fe (III) was applied in the fermentation of lactic acid in starch industry wastewater, the fermentation period reduced by 8 h and the output of L-lactic acid was 65.5 g/L, which was 3.3% more than that fermented without the carrier.

Xu, Wei; Wang, Peng; Chi, Guoda; Huang, Chenyong

2010-11-01

457

Microbial transformation of ginsenoside Rb1 to compound K by Lactobacillus paralimentarius.  

PubMed

In this study, the major ginsenoside Rb1 was transformed into the more pharmacologically active minor compound K by food grade Lactobacillus paralimentarius LH4, which was isolated from kimchi, a traditional Korean fermented food. The enzymatic reaction was analyzed by TLC, HPLC, and NMR. Using the cell-free enzyme of Lactobacillus paralimentarius LH4 at optimal conditions for 30 °C at pH 6.0, 1.0 mg ml(-1) ginsenoside Rb1 was transformed into 0.52 mg ml(-1) compound K within 72 h, with a corresponding molar conversion yield of 88 %. The cell-free enzyme hydrolyzed the two glucose moieties attached to the C-3 position and the outer glucose moiety attached to the C-20 position of the ginsenoside Rb1. The cell-free enzyme hydrolyzed the ginsenoside Rb1 along the following pathway: ginsenoside Rb1 ? gypenoside XVII and ginsenoside Rd ? ginsenoside F2 ? compound K. Our results indicate that Lactobacillus paralimentarius LH4 has the potential to be applied for the preparation of compound K in the food industry. PMID:23338962

Quan, Lin-Hu; Kim, Yeon-Ju; Li, Guan Hao; Choi, Kwang-Tea; Yang, Deok-Chun

2013-06-01

458

Lactobacillus ginsenosidimutans sp. nov., isolated from kimchi with the ability to transform ginsenosides.  

PubMed

Biotransformation of ginsenosides was examined using lactic acid bacteria isolated from several kinds of kimchi. A Gram-positive, facultatively anaerobic, non-motile, non-spore-forming, and rod-shaped lactic acid bacterial strain, designated EMML 3041(T), was determined to have ginsenoside-converting activity and its taxonomic position was investigated using a polyphasic approach. Strain EMML 3041(T) displayed ?-glucosidase activity that was responsible for its ability to transform ginsenoside Rb1 (one of the dominant active components of ginseng) to F2 via gypenoside XVII, ginsenoside Rb2 to compound Y via compound O, ginsenoside Rc to compound Mc via compound Mc1, and ginsenoside Rd to ginsenoside F2. On the basis of the 16S rRNA gene sequence similarity, strain EMML 3041(T) was shown to belong to the genus Lactobacillus and is closely related to Lactobacillus versmoldensis KU-3(T) (98.3 % sequence similarity). Polyphasic taxonomy study confirmed that the strain EMML 3041(T) represents a novel species, for which the name Lactobacillus ginsenosidimutans sp. nov. is proposed, with EMML 3041(T) (=KACC 14527(T) = JCM 16719(T)) as the type strain. PMID:23271644

Jung, Hae-Min; Liu, Qing-Mei; Kim, Jin-Kwang; Lee, Sung-Taik; Kim, Sun-Chang; Im, Wan-Taek

2013-04-01

459

Development of a Lactobacillus specific T-RFLP method to determine lactobacilli diversity in complex samples.  

PubMed

Terminal restriction fragment length polymorphism (T-RFLP) analysis has been widely used for studying microbial communities. However, most T-RFLP assays use 16S rDNA as the target and are unable to accurately characterize a microbial subpopulation. In this study, we developed a novel T-RFLP protocol based on Lactobacillus hsp60 to rapidly characterize and compare lactobacilli composition. The theoretical terminal restriction fragment (TRF) profiles were calculated from 769 Lactobacillus hsp60 sequences from online databases. In silico digestion with restriction endonucleases AluI and TacI on hsp60 amplicons generated 83 distinct TRF patterns, of which, 70 were species specific. To validate the assay, five previously sequenced lactobacilli were cultured independently, mixed at known concentrations and subjected to analysis by T-RFLP. All five strains generated the predicted TRFs and a qualitative consistent relationship was revealed. We performed the T-RFLP protocol on fecal samples from mice fed 6 different diets (n=4). Principal component analysis and agglomerative hierarchical clustering revealed that the lactobacilli community was strongly connected to dietary supplementation. Our study demonstrates the potential for using Lactobacillus specific T-RFLP to characterize lactobacilli communities in complex samples. PMID:22981747

Chen, Long; Teasdale, Matt T; Kaczmarczyk, Melissa M; Freund, Gregory G; Miller, Michael J

2012-11-01

460

Characterization of Lactobacillus isolates from fermented olives and their bacteriocin gene profiles.  

PubMed

Near one hundred isolates of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum from table olives were studied. Strains were genotyped by rep-PCR. Although the technique failed to differentiate some isolates at the species level, it proved a robust and easy procedure that could be useful for distinguishing between related strains of L. paraplantarum, L. pentosus and L. plantarum from a large pool of unrelated strains of these species. A PCR-based screening revealed the presence of the plantaricin encoding genes plnA, plnB, plnC, plnD, plnE/F, plnF, plnI, plnJ, plnK, plnG and plnN in most isolates of the three species. Sequences of bacteriocin genes present in L. paraplantarum and L. pentosus were homologous to L. plantarum genes. Through a discriminating analysis of the bacteriocin gene profiles, it was possible to establish a relationship between the origin of isolation and the LAB isolates, regardless of species. PMID:21925038

Hurtado, Albert; Ben Othman, Nada; Chammem, Nadia; Hamdi, Moktar; Ferrer, Sergi; Reguant, Cristina; Bordons, Albert; Rozès, Nicolas

2011-12-01

461

Minerals and organic nitrogen present in grape marc hydrolyzates enhance xylose consumption by Lactobacillus pentosus.  

PubMed

This work deals with the nutritional evaluation of grape marc hydrolyzates as fermentation medium for Lactobacillus pentosus. Usually, the fermentation of xylose and arabinose in the presence of glucose remains a primary obstacle for economical biomass conversion. The few microorganisms that can grow simultaneously on both pentose and hexose sugars contained in lignocellulosic feedstocks typically grow slowly and demonstrate marginal yields and productivities. Moreover, lignocellulosic hydrolyzates contain phenolic compounds and other components originated by the degradation of sugars that can inhibit lactic acid fermentation. However, in this case, grape marc hydrolyzates not only did not need a detoxification stage, but it also improved the xylose consumption by Lactobacillus pentosus with a faster and more efficient conversion of hemicellulosic sugars compared with synthetic media. After analysis of grape marc hydrolyzates, it was observed that minerals such as K (2,707 mg/L), Ca (3,681 mg/L), and Mg (198.5 mg/L) are present in higher concentration than those found in the general medium of Lactobacillus (1,705 mg/L of K, 58.3 mg/L of Ca, and 27.0 mg/L of Mg). Moreover, grape marc hydrolyzates contain an additional source of nitrogen (9.2 g/L) which, together with their elevated mineral concentration, improved lactic acid fermentation compared with synthetic media. PMID:18581267

Rivera, Oscar Manuel Portilla; Torrado, Ana María; Moldes, Ana Belén; Domínguez, José Manuel

2009-02-01

462

Evaluation of anti-Listeria meat borne Lactobacillus for biofilm formation on selected abiotic surfaces.  

PubMed

The ability of meat borne anti-Listeria Lactobacillus to form biofilms under different in vitro conditions and on abiotic surfaces was investigated. Biofilm formation by the adhesion to polystyrene microtiter plates was determined, this being higher for Lactobacillus curvatus CRL1532 and CRL705 and Lactobacillus sakei CRL1862. The physicochemical properties of the cell surface were relatively hydrophilic and acidic in character; L. sakei CRL1862 exhibiting the strongest autoaggregation. The adhesion of lactobacilli to stainless steel (SS) and polytetrafluoroethylene (PTFE) supports at 10°C was found to be maximal for L. sakei CRL1862 on SS after 6 days. When biofilm architecture was characterized by epifluorescence and SEM, L. sakei CRL1862 homogeneously covered the SS surface while cell clusters were observed on PTFE; the extracellular polymeric substance matrix adapted to the topography and hydrophilic/hydrophobic characteristics of each material. The feasibility of L. sakei CRL1862 to form biofilm on materials used in meat processing highlights its potential as a control strategy for Listeria monocytogenes biofilms. PMID:23933630

Pérez Ibarreche, Mariana; Castellano, Patricia; Vignolo, Graciela

2014-01-01

463

Membrane permeabilization in relation to inactivation kinetics of Lactobacillus species due to pulsed electric fields.  

PubMed

Membrane permeabilization due to pulsed electric field (PEF) treatment of gram-positive Lactobacillus cells was investigated by using propidium iodide uptake and single-cell analysis with flow cytometry. Electric field strength, energy input, treatment time, and growth phase affected membrane permeabilization of Lactobacillus plantarum during PEF treatment. A correlation between PEF inactivation and membrane permeabilization of L. plantarum cells was demonstrated, whereas no relationship was observed between membrane permeabilization and heat inactivation. The same results were obtained with a Lactobacillus fermentum strain, but the latter organism was more PEF resistant and exhibited less membrane permeabilization, indicating that various bacteria have different responses to PEF treatment. While membrane permeabilization was the main factor involved in the mechanism of inactivation, the growth phase and the acidity of the environment also influenced inactivation. By using flow cytometry it was possible to sort cells in the L. plantarum population based on different cell sizes and shapes, and the results were confirmed by image analysis. An apparent effect of morphology on membrane permeabilization was observed, and larger cells were more easily permeabilized than smaller cells. In conclusion, our results indicate that the ability of PEF treatment to cause membrane permeabilization is an important factor in determining inactivation. This finding should have an effect on the final choice of the processing parameters used so that all microorganisms can be inactivated and, consequently, on the use of PEF treatment as an alternative method for preserving food products. PMID:11425727

Wouters, P C; Bos, A P; Ueckert, J