Sample records for luminol chemiluminescence reaction

  1. Influence of the luminol chemiluminescence reaction on the confirmatory tests for the detection and characterization of bloodstains in forensic analysis

    Microsoft Academic Search

    V. R. D. Santos; W. X. Paula; E. Kalapothakis

    2009-01-01

    Preliminary tests for the detection of stains at crime scenes aim to focus the police work making them more efficient in the combat of criminality. The application of the luminol chemiluminescence reaction (3-aminoftalhidrazida) in presumptive tests for the detection of bloodstains is known for more than 40 years in forensic science. This reaction is based on the emission of light

  2. Flow-injection determination of ornidazole by chemiluminescence detection based on a luminol-ferricyanide reaction.

    PubMed

    Lv, Yi; Zhang, Zhujun; He, Deyong; Hu, Yufei

    2003-04-01

    A flow-injection analysis (FIA) with a chemiluminescence detection method was developed for the determination of ornidazole based on the inhibition intensity of chemiluminescence from the luminol-ferricyanide system. Under the condition of 1.0 x 10(-3) mol/L luminol and 5.0 x 10(-6) mol/L potassium ferricyanide, the response to the concentration of omidazole is linear from 0.2 microg ml(-1) to 10 microg ml(-1), and a detection limit of 0.05 microg ml(-1) can be obtained. This method has been successfully applied to the determination of omidazole in pharmaceutical preparations. PMID:12725405

  3. In vitro screening of Fe2+-chelating effect by a Fenton's reaction-luminol chemiluminescence system.

    PubMed

    Wada, Mitsuhiro; Komatsu, Hiroaki; Ikeda, Rie; Aburjai, Talal A; Alkhalil, Suleiman M; Kuroda, Naotaka; Nakashima, Kenichiro

    2014-11-01

    In vitro screening of a Fe(2+) -chelating effect using a Fenton's reaction-luminol chemiluminescence (CL) system is described. The luminescence between the reactive oxygen species generated by the Fenton's reaction and luminol was decreased on capturing Fe(2+) using a chelator. The proposed method can prevent the consumption of expensive seed compounds (drug discovery candidates) owing to the high sensitivity of CL detection. Therefore, the assay could be performed using small volumes of sample solution (150 ?L) at micromolar concentrations. After optimization of the screening conditions, the efficacies of conventional chelators such as ethylenediaminetetraacetic acid (EDTA), diethylentriaminepentaacetic acid (DETAPAC), deferoxamine, deferiprone and 1,10-phenanthroline were examined. EC50 values for these compounds (except 1,10-phenanthroline) were in the range 3.20 ± 0.87 to 9.57 ± 0.64 ?M (n = 3). Rapid measurement of the Fe(2+)-chelating effect with an assay run time of a few minutes could be achieved using the proposed method. In addition, the specificity of the method was discussed. PMID:24403191

  4. Luminol Chemiluminescence in Urine Analysis

    Microsoft Academic Search

    Ana María Jiménez Moreno; María José Navas Sánchez

    2006-01-01

    The purpose of the present review is to sketch out the scope of luminol chemiluminescence in human urine analysis. Practical considerations and experimental requirements are indicated. The literature revised covers the papers of analytical interest that have appeared in approximately the last six years.

  5. Flow injection chemiluminescence determination of captopril based on its enhancing effect on the luminol-ferricyanide/ferrocyanide reaction.

    PubMed

    Du, Jianxiu; Li, Yinhuan; Lu, Jiuru

    2002-01-01

    A new flow injection chemiluminescence method is described for the determination of captopril. It is based on the enhancing effect of captopril on the chemiluminescence reaction of luminol with potassium ferricyanide in alkaline solution in the presence of potassium ferrocyanide. The method allows the determination of captopril over 0.1-40 microg/mL range, with a relative standard deviation (SD) of 1.0% for the determination of 0.5 microg/mL captopril solution in 11 repeated measurements. The method was satisfactorily applied to the determination of captopril in commercial captopril tablets. The possible reaction mechanism is also discussed briefly. PMID:12164366

  6. Study on the chemiluminescence behavior of bovine serum albumin with luminol and its analytical application

    Microsoft Academic Search

    Xijuan Tan; Zhenghua Song; Donghua Chen; Zhuming Wang

    2011-01-01

    In this paper, the luminescence behavior of bovine serum albumin (BSA) and luminol was first studied by flow injection chemiluminescence (CL). It was found that the hyperchromic effect of luminol in the presence of BSA led to the acceleration of the electrons transferring rate of excited 3-aminophthalate, which greatly enhanced the CL intensity of luminol\\/dissolved oxygen reaction. The increments of

  7. Determination of cysteine and glutathione based on the inhibition of the dinuclear Cu(II)-catalyzed luminol-H2O2 chemiluminescence reaction.

    PubMed

    Chaichi, Mohammad Javad; Ehsani, Mahjoobeh; Khajvand, Tahereh; Golchoubian, Hamid; Rezaee, Ehsan

    2014-03-25

    The catalyzed luminol chemiluminescent reaction has received a great amount of attention because of its high sensitivity and low background signal which make the reaction an attractive analytical chemistry tool. The present study, introduces the beneficial catalytic effects of dinuclear Cu(II) complex [Cu2L2(TAE)]X2, where TAE=tetraacetylethane; L=N,N(')-dibenzylethylenediamine and X=ClO4 on the luminol chemiluminescent reaction as a novel probe for the determination of glutathione (GSH) and L-cysteine (CySH) in human serum and urine. The [Cu2L2(TAE)]X2 has exhibited highly efficient catalytic activity of luminol CL as an artificial peroxidase model at pH as low as 7.5 in water in the presence of H2O2?GSH and CySH can induce a sharp decrease in CL intensity from the [Cu2L2(TAE)]X2-catalyzed luminol system. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentrations of GSH and CySH in the range of 1.0×10(-7)-1.0×10(-4) M, with detection limits (S/N=3) of 2.7×10(-8) and 6.8×10(-8) M and RSD<4.2% (n=7) for GSH and CySH, respectively. PMID:24326259

  8. Determination of rutin by flow injection chemiluminescence method using the reaction of luminol and potassium hexacyanoferrate(III) with the aid of response surface methodology.

    PubMed

    Yang, Dan; Li, Haiyun; Li, Ziyuan; Hao, Zaibin; Li, Jianping

    2010-01-01

    A novel flow injection chemiluminescence (CL) method for the determination of rutin was reported. The proposed method was based on the enhanced effect of rutin on the chemiluminescence intensity of luminol and potassium hexacyanoferrate(III) reaction in NaOH medium. The variables of reaction system, such as luminol concentration, potassium hexacyanoferrate(III) concentration and NaOH concentration, were optimized with the aid of response surface methodology. For the responses prediction, a second-order polynomial model (SOPM) was applied. The optimal conditions for determination of rutin estimated by the model equation were as follows: NaOH concentration of 0.13?mol/L luminol concentration of 0.94 × 10(-6) ?mol/L, and K(3) Fe(CN)(6) concentration of 1.09 × 10(-4) ?mol/L. The theoretical increased ratio of CL intensity (IRI) predicted and actual IRI for 0.05?mg/L rutin under the above conditions were 99.40 and 99.74%, respectively. The SOPM model proved to be powerful for navigating the design space. Under the above optimum conditions, the increased IRI was linearly related to the concentration of rutin in the range from 0.008 to 0.100?mg/L with the regression equation IRI = 1948.20c + 5.24 (r = 0.9994) and in the range from 0.100 to 1.000?mg/L with the regression equation IRI = 1362.50 c + 61.94 (r = 0.9996). The detection limit (3?) was of 1.95 × 10(-3) ?mg/L. The sampling frequency of this method was 72/h. The method was used directly to determine rutin in tablets. PMID:19827000

  9. Ultrasensitive assay of azithromycin in medicine and bio-Fluids based on its enhanced luminol–H 2O 2 chemiluminescence reaction using flow injection technique

    Microsoft Academic Search

    Zhenghua Song; Changna Wang

    2003-01-01

    A simple flow injection chemiluminescence method with synergistic enhancement has been investigated for the rapid and sensitive determination of azithromycin. The synergistic action was significant in the chemiluminescence system of luminol–hydrogen peroxide with azithromycin as an enhancer. The enhanced chemiluminescence intensity was linear with the concentration of azithromycin over the range from 0.1 pg mL?1 to 1.0 ng mL?1 (r2=0.9988)

  10. Luminol-silver nitrate chemiluminescence enhancement induced by cobalt ferrite nanoparticles.

    PubMed

    Shi, Wenbing; Wang, Hui; Huang, Yuming

    2011-01-01

    CoFe(2)O(4) nanoparticles (NPs) could stimulate the weak chemiluminescence (CL) system of luminol and AgNO(3), resulting in a strong CL emission. The UV-visible spectra, X-ray photoelectron spectra and TEM images of the investigated system revealed that AgNO(3) was reduced by luminol to Ag in the presence of CoFe(2)O(4) NPs and the formed Ag covered the surface of CoFe(2)O(4) NPs, resulting in CoFe(2)O(4)-Ag core-shell nanoparticles. Investigation of the CL reaction kinetics demonstrated that the reaction among luminol, AgNO(3) and CoFe(2)O(4) NPs was fast at the beginning and slowed down later. The CL spectra of the luminol - AgNO(3) - CoFe(2)O(4) NPs system indicated that the luminophor was still an electronically excited 3-aminophthalate anion. A CL mechanism has been postulated. When the CoFe(2)O(4) NPs were injected into the mixture of luminol and AgNO(3), they catalyzed the reduction of AgNO(3) by luminol to produce luminol radicals and Ag, which immediately covered the CoFe(2)O(4) NPs to form CoFe(2)O(4)-Ag core-shell nanoparticles, and the luminol radicals reacted with the dissolved oxygen, leading to a strong CL emission. With the continuous deposition of Ag on the surface of CoFe(2)O(4) NPs, the catalytic activity of the core-shell nanoparticles was inhibited and a decrease in CL intensity was observed and also a slow growth of shell on the nanoparticles. PMID:21400653

  11. Determination of Monocrotophos Pesticide by Flow Injection Chemiluminescence Method Using Luminol-Hydrogen Peroxide System

    Microsoft Academic Search

    Jianxiu Du; Xiaoyu Liu; Jiuru Lu

    2003-01-01

    A simple flow injection chemiluminescence method is described for the determination of monocrotophos pesticide, based upon the direct reaction of monocrotophos with luminol and hydrogen peroxide in alkaline medium in the presence of an enhancer of sodium chloride. The CL intensity was linear related to monocrotophos concentration in the range of 2.0 × 10–1.0 × 10 g\\/mL with a detection limit of 7 × 10 g\\/mL. The relative

  12. Study on the chemiluminescence behavior of bovine serum albumin with luminol and its analytical application

    NASA Astrophysics Data System (ADS)

    Tan, Xijuan; Song, Zhenghua; Chen, Donghua; Wang, Zhuming

    2011-06-01

    In this paper, the luminescence behavior of bovine serum albumin (BSA) and luminol was first studied by flow injection chemiluminescence (CL). It was found that the hyperchromic effect of luminol in the presence of BSA led to the acceleration of the electrons transferring rate of excited 3-aminophthalate, which greatly enhanced the CL intensity of luminol/dissolved oxygen reaction. The increments of CL intensity were proportional to the concentrations of BSA with a linear range from 0.01 to 7 nmol L -1. It was also found that azithromycin could inhibit the CL intensity of luminol/BSA reaction. The decrements of CL intensity were logarithm over the concentrations of azithromycin ranging from 0.1 to 700 ng mL -1. At a flow rate of 2.0 mL min -1, a complete analytical process, which included sampling and washing, could be performed within 30 s with relative standard deviations of less than 3.1%. This proposed method was successfully applied in assaying azithromycin in pharmaceutical and human serum samples with recoveries from 91.0 to 104.3%. The possible luminescence mechanism of luminol/BSA/azithromycin reaction was discussed in detail by CL, UV and fluorescence methods.

  13. Study on the chemiluminescence behavior of bovine serum albumin with luminol and its analytical application.

    PubMed

    Tan, Xijuan; Song, Zhenghua; Chen, Donghua; Wang, Zhuming

    2011-06-01

    In this paper, the luminescence behavior of bovine serum albumin (BSA) and luminol was first studied by flow injection chemiluminescence (CL). It was found that the hyperchromic effect of luminol in the presence of BSA led to the acceleration of the electrons transferring rate of excited 3-aminophthalate, which greatly enhanced the CL intensity of luminol/dissolved oxygen reaction. The increments of CL intensity were proportional to the concentrations of BSA with a linear range from 0.01 to 7 nmol L(-1). It was also found that azithromycin could inhibit the CL intensity of luminol/BSA reaction. The decrements of CL intensity were logarithm over the concentrations of azithromycin ranging from 0.1 to 700 ng mL(-1). At a flow rate of 2.0 mL min(-1), a complete analytical process, which included sampling and washing, could be performed within 30s with relative standard deviations of less than 3.1%. This proposed method was successfully applied in assaying azithromycin in pharmaceutical and human serum samples with recoveries from 91.0 to 104.3%. The possible luminescence mechanism of luminol/BSA/azithromycin reaction was discussed in detail by CL, UV and fluorescence methods. PMID:21435942

  14. The effect of electrode material on the electrogenerated chemiluminescence of luminol

    SciTech Connect

    Vitt, J.E.; Johnson, D.C. (Ames Lab., IA (United States)); Engstrom, R.C. (Dept. of Chemistry, Univ. of South Dakota, Vermillion, SD (US))

    1991-06-01

    This paper reports on the oxidation of luminol and its concomitant electrogenerated chemiluminescence (ECL) which were studied at several electrode materials by voltammetry and chronoamperometry. The ECL intensity (I{sub ECL}) was inversely related to the activity of the electrodes. The lowest I{sub ECL}) was measured when luminol was oxidized to 3-aminophthalate (n {approx equal}4 eq mol{sup {minus}1}) at a nearly mass-transport limited rate at glassy carbon. The ECL kinetics were studied and the order of the reaction with respect to luminol was 3/2 at concentrations to ca. 1 mM when O{sub 2} was the coreactant. In the presence of H{sub 2}O{sub 2}, the ECL reaction was first order with respect to luminol. A reaction mechanism is proposed that is consistent with the inetic data and the inverse relationship between electrode activity and I{sub ECL}. The implications of these results are discussed with respect to imaging the spatial distribution of current density at electrode surfaces, including that of PbO{sub 2} films activated by adsorbed Bi(V). A value of 6.6 {times} 10{sup {minus}6} cm{sup 2} s{sup {minus}1} was determined for the diffusion coefficient of luminol in 0.1M NaOH.

  15. Alkaline Hydrolysis of Nitroglycerin and Activation of Luminol Chemiluminescence

    Microsoft Academic Search

    Yu. B. Tsaplev

    2004-01-01

    Alkaline hydrolysis of nitroglycerin (G) was studied using the chemiluminescent reaction of the hydrolysis products with 4-dimethylaminophthalhydrazide (L). The chemiluminescence kinetics follows the pseudo-unimolecular law, with the rate constant k1 proportional to [OH–]. The apparent bimolecular constant k2 = k1\\/[OH–] is equal to 0.021 l mol–1 s–1. The chemiluminescence quantum yield per one nitroglycerin molecule ?G = (1.3 ± 0.3)

  16. Reactive oxygen species and human spermatozoa: analysis of the cellular mechanisms involved in luminol- and lucigenin-dependent chemiluminescence.

    PubMed

    Aitken, R J; Buckingham, D W; West, K M

    1992-06-01

    We have shown that human spermatozoa generate and release reactive oxygen species that can be detected by chemiluminescence techniques. Analysis of the cellular mechanisms responsible for this activity suggests that the probe, luminol, undergoes an intracellular dioxygenation reaction mediated by hydrogen peroxide and a sperm peroxidase located within the acrosome. Support for this model included the following observations: (1) the luminol-dependent signal could be suppressed with peroxidase inhibitors, phenylhydrazine and sodium azide; (2) this suppression could be reversed by the addition of an azide-insensitive peroxidase, horse radish peroxidase (HRP); (3) inhibition of intracellular superoxide dismutase (SOD) with potassium cyanide (KCN) suppressed the luminol signal; (4) peroxidase activity could be detected in purified populations of human spermatozoa with 3,3',5,5' tetramethylbenzidine (TMB); (5) this peroxidase was active at the pH prevailing within the acrosomal vesicle; and (6) peroxidase activity and luminol-dependent chemiluminescence were minimal in spermatozoa exhibiting a congenital absence of acrosomes. Human spermatozoa could also generate lucigenin-dependent chemiluminescent signals that could neither be suppressed with peroxidase inhibitors nor enhanced by the addition of peroxidase. However, these signals could be enhanced by suppression of intracellular SOD with KCN or inhibited by exogenous SOD, suggesting that lucigenin was responding to superoxide anion released into the extracellular space. The ability of chemiluminescent techniques to detect and discriminate the production of superoxide and hydrogen peroxide by spermatozoa should facilitate the further analysis of reactive oxygen species as mediators of normal and abnormal human sperm function. PMID:1338331

  17. A study of the chemiluminescence behavior of myoglobin with luminol and its analytical applications.

    PubMed

    Song, Zhenghua; Wang, Lin; Hou, Shuang

    2004-01-01

    A chemiluminescence signal at 425 nm was observed when ferric state myoglobin was mixed with luminol in alkaline medium. Because the signal was remarkably enhanced in the presence of Fe(CN)6(4-), analytical applications were investigated in a flow-injection system. The increase in chemiluminescence was linearly dependent on myoglobin concentration in the range 0.1 to 100 nmol L(-1), and the limit of detection was 0.04 nmol L(-1) with relative standard deviation 3.2% (3 sigma). It was also found that binding of Mb with the ligands CN-, SCN-, and F- significantly inhibited the chemiluminescence reaction. The linear dynamic ranges for the ligands were 1.0-300.0, 0.1-3.0, and 0.5-100.0 nmol L(-1), and the limits of detection (S/N=3) 0.4, 0.04, and 0.2 nmol L(-1), for F-, CN-, and SCN-, respectively. The relative standard deviations were 5.32%, 6.13%, and 3.38% for 0.1 nmol L(-1) CN-, 0.5 nmol L(-1) SCN-, and 1.0 nmol L(-1) F-, respectively. At a flow rate of 2.0 mL min(-1) the assay could be accomplished in 1 min, including sampling and washing. The method has been successfully applied to the determination of myoglobin in human urine and F- in water samples. A possible mechanism of chemiluminescence production by myoglobin and luminol is presented. PMID:14663544

  18. Determination of ATP via the photochemical generation of hydrogen peroxide using flow injection luminol chemiluminescence detection.

    PubMed

    Pérez-Ruiz, Tomás; Martínez-Lozano, Carmen; Tomás, Virginia; Martín, Jesús

    2003-09-01

    The determination of ATP using the coupling between a photochemical reaction and a chemiluminescence reaction in a flow injection (FI) system is described. The method is based on the reaction of glucose with ATP catalyzed by hexokinase and Mg(2+) ions. The glucose that is not consumed by ATP is subsequently photooxidized using 9,10-anthraquinone-2,6-disulfonate as a sensitizer. The hydrogen peroxide produced in the photochemical reaction is monitored through the chemiluminescence reaction with luminol catalyzed by hematine. There is a linear relationship between the decrease in the chemiluminescence response and the ATP concentration at a constant concentration of glucose. Under the optimum conditions, the calibration graph is linear in the range 0.20-50.5 mg L(-1) with a throughput of 25 samples per hour and relative standard deviations between +/-0.62 and +/-1.42%. The limit of detection is 0.07 mg L(-1). The method was used for the determination of ATP in pharmaceuticals, milk, and soils. PMID:12851734

  19. Pharmacokinetic of pseudoephedrine in rat serum with luminol-pepsin chemiluminescence system by flow injection analysis.

    PubMed

    Luo, Kai; Li, Yajuan; Zheng, Xiaohui; Song, Zhenghua

    2015-02-01

    Pepsin (Pep) accelerated the electron transferring rate of excited 3-aminophathlate and enhanced luminol-dissolved oxygen chemiluminescence (CL) intensity, and the flow injection (FI) luminol-Pep CL system was first developed. It was found that the CL intensity of luminol-Pep reaction could be remarkably inhibited by pseudoephedrine (PE); the decrement of CL intensity was linear to the logarithm of PE concentration in the range of 0.1?100.0 nmol L(-1) with a detection limit of 0.03 nmol mL(-1) (3?). At a flow rate of 2.0 mL min(-1), the complete process including washing and sampling was performed within 40 s, offering a sample throughput of 90 h(-1). This proposed method was successfully applied to determining PE in rat serum for 18 h after intragastric administration with the elimination ratio of 42.34 % and recoveries from 90.3 to 110.6 %. The pharmacokinetic results showed that PE could be rapidly absorbed into serum with peak concentration (C max) of 1.45?±?0.18 g L(-1) at the time (T max) of 1.49?±?0.02 h; the absorption half-life (0.35?±?0.04 h), elimination half-life (1.86?±?0.24 h), the area under curve (109.81?±?6.03 mg L(-1) h(-1)), mean residence time (3.82?±?0.27 h), and elimination rate constant (2.26?±?0.23 L g(-1) h(-1)) in rats vivo were derived, respectively. The possible CL mechanism of luminol-Pep-PE reaction was discussed by FI-CL, fluorescence, and molecular docking (MD) methods. PMID:25427591

  20. Determination of norfloxacin in pharmaceuticals, human serum, and urine using a luminol—dissolved oxygen chemiluminescence system

    Microsoft Academic Search

    X. D. Shao; H. Y. Liu; X. F. Gao; W. Q. Chen; Z. H. Song

    2007-01-01

    A sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved\\u000a oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence\\u000a intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from\\u000a 0.4 ng mL?1 to 400.0 ng mL?1

  1. Effect of oxidatively modified and non-modified human serum albumin on luminol-dependent chemiluminescence of human peripheral blood leukocytes stimulated with opsonized zymosan.

    PubMed

    Piryazev, A P; Azizova, A P; Aseichev, A V; Sergienko, V I

    2014-07-01

    We studied the effects of native and oxidized human serum albumin on luminol-dependent chemiluminescence of human peripheral blood leukocytes stimulated with opsonized zymosan. Human serum albumin was added simultaneously with opsonized zymosan at the beginning of the chemiluminescent reaction. Otherwise, leukocytes were incubated with human serum albumin at 37°C for various periods before addition of opsonized zymosan. Oxidized human serum albumin was obtained by the method of metal-catalyzed oxidation. In control to non-modified albumin, oxidized albumin produced an inhibitory effect on luminol-dependent chemiluminescence of leukocytes. These changes were observed in experiments with addition of oxidized albumin at the beginning of a chemiluminescent reaction and after incubation of study agent with cells. PMID:25065314

  2. Flow-injection determination of isoniazid using sodium dichloroisocyanurate- and trichloroisocyanuric acid-luminol chemiluminescence systems.

    PubMed

    Safavi, A; Karimi, M A; Hormozi Nezhad, M R

    2004-06-01

    A chemiluminescent (CL) method for the determination of isoniazid is described. The method is based on the CL generated during the oxidation of luminol by sodium dichloroisocyanurate (SDCC) and trichloroisocyanuric acid (TCCA) in alkaline medium. It was found that isoniazid greatly enhances this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of isoniazid has been proposed in this paper. The detection limits were 2 and 3 ng ml(-1) isoniazid for the SDCC-luminol and TCCA-luminol CL systems, respectively. The relative CL intensity was linear with the isoniazid concentration in the range of 4-100 and 100-200 ng ml(-1) for the SDCC-luminol CL system, and 6-200 and 200-1000 ng ml(-1) for the TCCA-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official methods and demonstrated good accuracy and precision. PMID:15178311

  3. Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurements

    Microsoft Academic Search

    Eduardo Lissi; Marta Salim-Hanna; Carlos Pascual; Maria D. del Castillo

    1995-01-01

    The effect of antioxidants and biological fluids on the intensity of luminol induced chemiluminescence by radicals derived from the thermolysis of 2,2?-azo-bis(2-amidinopropane) has been employed to monitor TRAP and TOTAL ANTIOXIDANT REACTIVITY (TAR) levels. The latter parameter, which considers not only the quantity of oxidants but also their reactivity, is considered a potentially more useful index of the antioxidant status

  4. Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination

    PubMed Central

    Asgher, Muhammad; Yaqoob, Mohammad; Nabi, Abdul; Siddiqi, Abdul Rauf

    2014-01-01

    Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•?) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•?) and hydrogen peroxide (H2O2). Highly reactive hydroxyl radicals (•OH) were produced via dismutation of H2O2 by catalyst (Cu2+). The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n = 6) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05??g?mL?1, respectively. The sample throughput of 70?h?1 for vitamins A and C and 30?h?1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50??g?mL?1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n = 3) in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values. PMID:25614739

  5. Determination of l-thyroxine in pharmaceutical preparations by flow injection analysis with chemiluminescence detection based on the enhancement of the luminol-KMnO4 reaction in a micellar medium.

    PubMed

    Cao, Juntao; Wang, Hui; Liu, Yanming

    2015-04-01

    A novel flow injection chemiluminescence (CL) method for the determination of l-thyroxine in the presence of cethyltrimethylammonium bromide (CTMAB) surfactant micelles is developed. The method is based on the significant signal enhancement of l-thyroxine on the luminol-KMnO4 system in alkaline solution sensitized by CTMAB. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimum conditions, the net CL intensity versus l-thyroxine concentration was linear in the range of 5.0×10(-8)-3.0×10(-6)mol/L with the detection limit of 8.9×10(-9)mol/L. The sample throughput is calculated to be 140samples/h and the relative standard deviations (RSDs) for 13 replicate determination of 1.0×10(-6)l-thyroxine is 1.1%. The proposed method was successfully applied for the determination of l-thyroxine in pharmaceutical preparations with satisfactory recoveries in the range of 93.9-105.2%. This rapid, sensitive, and high throughput method would provide a new tool for l-thyroxine analysis. PMID:25594210

  6. Carbon nanofiber-based luminol-biotin probe for sensitive chemiluminescence detection of protein.

    PubMed

    Baj, Stefan; Krawczyk, Tomasz; Pradel, Natalia; Azam, Md Golam; Shibata, Takayuki; Dragusha, Shpend; Skutil, Krzysztof; Pawlyta, Miroslawa; Kai, Masaaki

    2014-01-01

    A carbon nanofiber-based luminol-biotin probe was synthesized for the sensitive chemiluminescence (CL) detection of a target protein by grafting luminol and biotin onto an oxidized carbon nanofiber. This carbon nanofiber was prepared by chemical vapor-deposition with methane in the presence of the Ni-Cu-MgO catalyst, which was followed by oxidization with HNO3-H2SO4 to produce a carboxyl group on the surface of the nanofiber. The material was grafted with luminol and biotin by means of a standard carbodiimide activation of COOH groups to produce corresponding amides. The substance was water-soluble and thus could be utilized as a sensitive CL probe for a protein assay. The probe showed highly specific affinity towards the biotin-labeled antibody via a streptavidin-biotin interaction. The detection limit for this model assay was approximately 0.2 pmol of the biotinized IgG spotted on a polyvinylidene fluoride (PVDF) membrane. Nonspecific binding to other proteins was not observed. Therefore, the synthesized carbon nanofiber-based CL probe may be useful for a sensitive and specific analysis of the target protein. PMID:25382040

  7. Determination of nitrogen dioxide with a chemiluminescent aerosol detector

    SciTech Connect

    Mikuska, P.; Vecera, Z. [Institute of Analytical Chemistry, Brno (Czechoslovakia)

    1992-09-15

    A modified detector is described for use in the determination of nitrogen dioxide via reaction with luminol. Chemiluminescence of the aerosol particles formed by crossed streams of the analyte and an alkaline luminol solution was observed by a photomultiplier.

  8. Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate.

    PubMed

    Tao, Xiaoqi; Wang, Wenjun; Wang, Zhanhui; Cao, Xingyuan; Zhu, Jinghui; Niu, Lanlan; Wu, Xiaoping; Jiang, Haiyang; Shen, Jianzhong

    2014-06-01

    In this study, a high sensitivity chemiluminescence enzyme immunoassay (CLEIA) based on novel enhancers was developed. Under optimal conditions, we developed an enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP-C) in the presence of 3-(10'-phenothiazinyl) propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP) as enhancers. The limit of detection of the newly prepared chemiluminescent cocktail for HRP was 0.33?pg/well, which is lower than that of commercial Super Signal substrate. The results showed that this novel chemiluminescent cocktail can significantly increase the light output of HRP-catalyzed ECR, which can be translated into a corresponding improvement in sensitivity. Similar improvements were observed in CLEIA for the determination of chloramphenicol in milk. In addition, the ECR of N-azoles as secondary enhancer was also presented. PMID:23785024

  9. Effect of aggregated silver nanoparticles on luminol chemiluminescence system and its analytical application

    NASA Astrophysics Data System (ADS)

    Qi, Yingying; Li, Baoxin; Xiu, Furong

    2014-07-01

    We found that after silver nanoparticles (AgNPs) aggregated, its catalytic activity on luminol CL reaction obviously changed, and the change characteristic was closely related to the sizes of AgNPs. UV-visible spectra, X-ray photoelectron spectra, zeta potential and transmission electron microscopy studies were carried out to investigate the CL effect mechanism. The different CL responses of aggregated AgNPs with different size were suggested to be due to the two effects of quantum size and electron density in nanoparticle's conduction bands, and which one played a major role. The poisonous organic contaminants such as anilines, could induce the aggregation of AgNPs, were observed to affect effectively the luminol-H2O2-7 nm and 15 nm AgNPs CL systems and were detectable by use of a flow injection method with the enhanced or inhibited CL detection.

  10. Luminol functionalized gold nanoparticles as colorimetric and chemiluminescent probes for visual, label free, highly sensitive and selective detection of minocycline.

    PubMed

    He, Yi; Peng, Rufang

    2014-11-14

    In this work, luminol functionalized gold nanoparticles (LuAuNPs) were used as colorimetric and chemiluminescent probes for visual, label free, sensitive and selective detection of minocycline (MC). The LuAuNPs were prepared by simple one-pot reduction of HAuCl? with luminol, which exhibited a good chemiluminescence (CL) activity owing to the presence of luminol molecules on their surface and surface plasmon resonance absorption. In the absence of MC, the color of LuAuNPs was wine red and their size was relatively small (?25 nm), which could react with silver nitrate, producing a strong CL emission. Upon the addition of MC at acidic buffer solutions, the electrostatic interaction between positively charged MC and negatively charged LuAuNPs caused the aggregation of LuAuNPs, generating a purple or blue color. Simultaneously, the aggregated LuAuNPs did not effectively react with silver nitrate, producing a weak CL emission. The signal change was linearly dependent on the logarithm of MC concentration in the range from 30 ng to 1.0 ?g for colorimetric detection and from 10 ng to 1.0 ?g for CL detection. With colorimetry, a detection limit of 22 ng was achieved, while the detection limit for CL detection modality was 9.7 ng. PMID:25327146

  11. Chitosan-induced Au/Ag nanoalloy dispersed in IL and application in fabricating an ultrasensitive glucose biosensor based on luminol-H?O?-Cu²?/IL chemiluminescence system.

    PubMed

    Chaichi, M J; Alijanpour, S O

    2014-11-01

    A novel glucose biosensor based on the chemiluminescence (CL) detection of enzymatically generated hydrogen peroxide (H?O?) was constructed by one covalent immobilization of glucose oxidase (GOD) in glutaraldehyde-functionalized glass cell. In following, chitosan-induced Au/Ag nanoparticles dispersed in ion liquid (IL) were synthesised and immobilized on it. Herein, chitosan molecules acted as both the reducing and stabilizing agent for the preparation of NPs and also, as a coupling agent GOD and Au/Ag alloy NPs. In addition to catalyze luminol CL reaction, these NPs offered excellent catalytic activity toward hydrogen peroxide generation in enzymatic reaction between GOD and glucose. The used IL in fabrication of biosensor increased its stability. Also, IL alongside Cu(2+) accelerated enzymatic and CL reaction kinetic, and decreased luminol CL reaction optimum pH to 7.5 which would enable sensitive and precision determination of glucose. Under optimum condition, linear response range of glucose was found to be 1.0 × 10(-6)-7.5 × 10(-3)M, and detection limit was 4.0 × 10(-7)M. The CL biosensor exhibited good storage stability, i.e., 90% of its initial response was retained after 2 months storage at pH 7.0. The present CL biosensor has been applied satisfactory to analysis of glucose in real serum and urine samples. PMID:25086323

  12. Luminol Test

    NSDL National Science Digital Library

    Oregon Museum of Science and Industry

    1997-01-01

    Learners mix a solution containing luminol and copper with a fake blood solution. A chemical reaction between the luminol solution and fake blood (hydrogen peroxide) show learners a blue glow. Forensic scientists spray luminol onto surfaces at crime scenes to detect invisible blood stains. Luminol solution reacts with blood, changing the structure of the molecule and temporarily adding energy. This energy causes the luminol to glow. This activity is used in the Crime Science Chemistry unit in OMSI's Chemistry Lab. Supply estimates are for 100 learners. For safety reasons, this activity should be conducted as a demonstration for younger audiences.

  13. Sensitive assay of hexythiazox residue in citrus fruits using gold nanoparticles-catalysed luminol-H2O2 chemiluminescence.

    PubMed

    Khajvand, Tahereh; Chaichi, Mohammad Javad; Colagar, Abasalt Hosseinzadeh

    2015-04-15

    A new sensitive chemiluminescence (CL) procedure for the detection of hexythiazox (HXTZ) is presented, based on the quenching effect of the HXTZ in the luminol-H2O2 system using gold nanoparticles (GNPs) as a catalyst. The Box-Behnken design matrix and response surface methodology (RSM) have been applied in designing the experiments for studying the interactive effects of the three most important variables pH, luminol, and H2O2 concentrations on the CL intensity of luminol catalysed by GNPs. Under the optimal conditions, the CL intensity was linear with HXTZ concentration in the range of 0.017-0.42 ?g mL(-1), and the limit of detection (LoD) was 0.011 ?g mL(-1). The procedure has been successfully applied to the detection of HXTZ residues in citrus fruits and water samples at trace levels. Mean recoveries obtained were between 84.0% and 95.3%, with a repeatability precision of <6%. Meanwhile, the possible mechanism of the inhibited CL intensity was discussed. PMID:25466053

  14. Flow injection determination of p-aminophenol at trace level using inhibited luminol-dimethylsulfoxide-NaOH-EDTA chemiluminescence.

    PubMed

    Xu, Hong; Duan, Chun-Feng; Zhang, Zhi-Feng; Chen, Jia-Yi; Lai, Chun-Ze; Lian, Mei; Liu, Li-Juan; Cui, Hua

    2005-01-01

    A novel flow injection procedure was developed for the determination of p-aminophenol (PAP) based on the inhibition by PAP of the chemiluminescence from luminol-dimethylsulfoxide (DMSO)-NaOH-EDTA system. The method has merits of higher sensitivity, wider linear range, simpler procedure, and a more rapid analyzing speed. It is applicable for the determination of PAP in the range of 2.5 x 10(-10)-5.0 x 10(-8) g mL(-1) with a detection limit of 1.9 x 10(-10) g mL(-1). The relative standard deviation (RSD) for 5.0 x 10(-9) g mL(-1) PAP is 0.78% (n=15). The method has been successfully used to determine PAP in industrial wastewaters and environmental waters. Additionally, the inhibition mechanism was also discussed briefly. PMID:15644248

  15. Research and development of a luminol-carbon monoxide flow system

    NASA Technical Reports Server (NTRS)

    Thomas, R. R.

    1977-01-01

    Adaption of the luminol-carbon monoxide injection system to a flowing type system is reported. Analysis of actual wastewater samples was carried out and revealed that bacteria can be associated with particles greater than 10 microns in size in samples such as mixed liquor. Research into the luminol reactive oxidation state indicates that oxidized iron porphyrins, cytochrome-c in particular, produce more luminol chemiluminescence than the reduced form. Correlation exists between the extent of porphyrin oxidation and relative chemiluminescence. In addition, the porphyrin nucleus is apparently destroyed under the current chemiluminescent reaction conditions.

  16. Flow injection determination of adenine at trace level based on luminol–K 2Cr 2O 7 chemiluminescence in a micellar medium

    Microsoft Academic Search

    Liu Erbao; Xue Bingchun

    2006-01-01

    A novel flow injection chemiluminescence (CL) analysis method for the determination of adenine in the presence of sodium dodecylbenzene sulfonate (SDBS) surfactant micelles is described. This method is based on the luminescent properties of the luminol–K2Cr2O7–adenine in alkaline medium sensitized by SDBS. The optimized experimental conditions were evaluated and the possible mechanism was discussed by examining CL emission spectrum and

  17. Study on the reaction mechanism and the static injection chemiluminescence method for detection of acetaminophen.

    PubMed

    Wu, Yongjun; Zhang, Huili; Yu, Songcheng; Yu, Fei; Li, Yanqiang; Zhang, Hongquan; Qu, Lingbo; Harrington, Peter de B

    2013-01-01

    Acetaminophen, also called paracetamol, is found in Tylenol, Excedrin and other products as over-the-counter medicines. In this study, acetaminophen as a luminol signal enhancer was used in the chemiluminescence (CL) substrate solution of horseradish peroxidase (HRP) for the first time. The use of acetaminophen in the luminol-HRP-H2O2 system affected not only the intensity of the obtained signal, but also its kinetics. It was shown that acetaminophen was to be a potent enhancer of the luminol-HRP-H2O2 system. A putative enhancement mechanism for the luminol-H2O2-HRP-acetaminophen system is presented. The resonance of the nucleophilic amide group and the benzene ring of acetaminophen structure have a great effect on O-H bond dissociation energy of the phenol group and therefore on phenoxyl radical stabilization. These radicals act as mediators between HRP and luminol in an electron transfer reaction that generates luminol radicals and subsequently light emission, in which the intensity of CL is enhanced in the presence of acetaminophen. In addition, a simple method was developed to detect acetaminophen by static injection CL based on the enhanced CL system of luminol-H2O2-HRP by acetaminophen. Experimental conditions, such as pH and concentrations of substrates, have been examined and optimized. The proposed method exhibited good performance, the linear range was from 0.30 to 7.5 mM, the relative standard deviation was 1.86% (n = 10), limit of detection was 0.16 mM and recovery was 99 ± 4%. PMID:23408702

  18. Enhancement of electrogenerated chemiluminescence of luminol by ascorbic acid at gold nanoparticle/graphene modified glassy carbon electrode

    NASA Astrophysics Data System (ADS)

    Dong, Yongping; Gao, Tingting; Zhou, Ying; Chu, Xiangfeng; Wang, Chengming

    2015-01-01

    Gold nanoparticle/graphene (GNP/GR) nanocomposite was one-pot synthesized from water soluble graphene and HAuCl4 by hydrothermal method and characterized by TEM, Raman spectroscopy, XRD, XPS, UV-vis spectroscopy, and electrochemical impedance spectroscopy (EIS). Electrogenerated chemiluminescence (ECL) of luminol was investigated at the GNP/GR modified glassy carbon electrode (GNP/GR/GCE) and the GNP modified glassy carbon electrode (GNP/GCE) in aqueous solution respectively. The results revealed that one strong anodic ECL peak could be observed at ?0.8 V at two modified electrodes compared with that at the bare electrode. The intensity of the anodic ECL at the GNP/GR/GCE is weaker than that at the GNP/GCE, which should be due to the synergic effect of the enhancing effect of gold nanoparticles and the inhibiting effect of graphene on anodic luminol ECL. One strong cathodic ECL peak located at ?-0.8 V could be observed at the GNP/GR/GCE but not at the GNP/GCE, which should be result from the adsorbed oxygen at the graphene film. In the presence of ascorbic acid, the anodic ECL at the GNP/GR/GCE was enhanced more than 8-times, which is more apparent than that at the GNP/GCE. Whereas, the cathodic ECL peak was seriously inhibited at the GNP/GR/GCE. The enhanced ECL intensity at the GNP/GR/GCE varied linearly with the logarithm of ascorbic acid concentration in the range of 1.0 × 10-8 to 1.0 × 10-6 mol L-1 with a detection limit of 1.0 × 10-9 mol L-1. The possible ECL mechanism was also discussed.

  19. Enhancement of electrogenerated chemiluminescence of luminol by ascorbic acid at gold nanoparticle/graphene modified glassy carbon electrode.

    PubMed

    Dong, Yongping; Gao, Tingting; Zhou, Ying; Chu, Xiangfeng; Wang, Chengming

    2015-01-01

    Gold nanoparticle/graphene (GNP/GR) nanocomposite was one-pot synthesized from water soluble graphene and HAuCl? by hydrothermal method and characterized by TEM, Raman spectroscopy, XRD, XPS, UV-vis spectroscopy, and electrochemical impedance spectroscopy (EIS). Electrogenerated chemiluminescence (ECL) of luminol was investigated at the GNP/GR modified glassy carbon electrode (GNP/GR/GCE) and the GNP modified glassy carbon electrode (GNP/GCE) in aqueous solution respectively. The results revealed that one strong anodic ECL peak could be observed at ?0.8 V at two modified electrodes compared with that at the bare electrode. The intensity of the anodic ECL at the GNP/GR/GCE is weaker than that at the GNP/GCE, which should be due to the synergic effect of the enhancing effect of gold nanoparticles and the inhibiting effect of graphene on anodic luminol ECL. One strong cathodic ECL peak located at ?-0.8 V could be observed at the GNP/GR/GCE but not at the GNP/GCE, which should be result from the adsorbed oxygen at the graphene film. In the presence of ascorbic acid, the anodic ECL at the GNP/GR/GCE was enhanced more than 8-times, which is more apparent than that at the GNP/GCE. Whereas, the cathodic ECL peak was seriously inhibited at the GNP/GR/GCE. The enhanced ECL intensity at the GNP/GR/GCE varied linearly with the logarithm of ascorbic acid concentration in the range of 1.0 × 10(-8) to 1.0 × 10(-6)mol L(-1) with a detection limit of 1.0 × 10(-9) mol L(-1). The possible ECL mechanism was also discussed. PMID:25022493

  20. Flow-injection chemiluminescence determination of cloxacillin in water samples and pharmaceutical preparation by using CuO nanosheets-enhanced luminol-hydrogen peroxide system

    NASA Astrophysics Data System (ADS)

    Khataee, Alireza; Iranifam, Mortaza; Fathinia, Mehrangiz; Nikravesh, Mina

    2015-01-01

    In this paper, a rapid and sensitive flow-injection chemiluminescence (flow-CL) system was developed for the determination of cloxacillin sodium in environmental water samples and pharmaceutical preparations. The method was based on the enhancement effect of cloxacillin sodium on the CL reaction of luminal-H2O2-CuO nanosheets (NSs) in alkaline medium. The CuO nanosheets were synthesized using a green sonochemical method. The physical properties of the synthesized CuO nanosheets were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses. The influences of various experimental factors such as H2O2, NaOH, luminol and CuO nanosheets concentrations were investigated. Under the optimum conditions, the enhanced CL intensity was linearly related to the concentration of cloxacillin sodium in the range of the 0.05-30.00 mg L-1 with a correlation coefficient of 0.995. The corresponding detection limit (3?) was calculated to be 0.026 mg L-1. The relative standard deviation (RSD) of the developed method was 2.21% with 11 repeated measurements of 4.00 mg L-1 cloxacillin sodium. Also, a total analysis time per sample was 30 s which confirmed the rapidity of the proposed method. The analytical applicability of the proposed CL system was assessed by determining cloxacillin sodium in spiked environmental water samples and pharmaceutical preparation. Furthermore, the possible mechanism of CL reaction was discussed.

  1. Flow-injection chemiluminescence determination of cloxacillin in water samples and pharmaceutical preparation by using CuO nanosheets-enhanced luminol-hydrogen peroxide system.

    PubMed

    Khataee, Alireza; Iranifam, Mortaza; Fathinia, Mehrangiz; Nikravesh, Mina

    2015-01-01

    In this paper, a rapid and sensitive flow-injection chemiluminescence (flow-CL) system was developed for the determination of cloxacillin sodium in environmental water samples and pharmaceutical preparations. The method was based on the enhancement effect of cloxacillin sodium on the CL reaction of luminal-H?O?-CuO nanosheets (NSs) in alkaline medium. The CuO nanosheets were synthesized using a green sonochemical method. The physical properties of the synthesized CuO nanosheets were characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses. The influences of various experimental factors such as H?O?, NaOH, luminol and CuO nanosheets concentrations were investigated. Under the optimum conditions, the enhanced CL intensity was linearly related to the concentration of cloxacillin sodium in the range of the 0.05-30.00 mg L(-1) with a correlation coefficient of 0.995. The corresponding detection limit (3?) was calculated to be 0.026 mg L(-1). The relative standard deviation (RSD) of the developed method was 2.21% with 11 repeated measurements of 4.00 mg L(-1) cloxacillin sodium. Also, a total analysis time per sample was 30 s which confirmed the rapidity of the proposed method. The analytical applicability of the proposed CL system was assessed by determining cloxacillin sodium in spiked environmental water samples and pharmaceutical preparation. Furthermore, the possible mechanism of CL reaction was discussed. PMID:25014643

  2. The chemiluminescence determination of 2-chloroethyl ethyl sulfide using luminol-AgNO3-silver nanoparticles system.

    PubMed

    Maddah, Bozorgmehr; Shamsi, Javad; Barsang, Mehran Jam; Rahimi-Nasrabadi, Mehdi

    2015-05-01

    A highly sensitive chemiluminescence (CL) method for the determination of 2-chloroethyl ethyl sulfide (2-CEES) was presented. It was found that 2-chloroethyl ethyl sulfide (2-CEES) could inhibit the CL of the luminol-AgNO3 system in the presence of silver nanoparticles in alkaline solution, which made it applicable for determination of 2-CEES. The presented method is simple, convenient, rapid and sensitive. Under the optimized conditions, the calibration curve was linear in the range of 0.0001-1ngmL(-1), with the correlation coefficient of 0.992; while the limit of detection (LOD), based on signal-to-noise ratio (S/N) of 3, was 6×10(-6)ngmL(-1). Also, the relative standard deviation (RSD, n=5) for determination of 2-CEES (0.50ngmL(-1)) was 3.1%. The method was successfully applied for the determination of 2-CEES in environmental aqueous samples. PMID:25703367

  3. Aircraft measurements of nitrogen dioxide and peroxyacyl nitrates using luminol chemiluminescence with fast capillary gas chromatography

    SciTech Connect

    Gaffney, J.S.; Marley, N.A. [Argonne National Lab., IL (United States). Environmental Research Div.; Drayton, P.J. [Univ. of Chicago, IL (United States). Dept. of Geophysical Sciences

    1997-09-01

    Peroxyacyl nitrates (PANs) and nitrogen dioxide (NO{sub 2}) are important trace gas species associated with photochemical air pollution. The PANs are in thermal equilibrium with the peroxyacetyl radical and NO{sub 2}. Because PANs are trapped peroxy radicals, they are an important indicator species of the photochemical age of an air parcel, as well as being a means of long-range transporting of NO{sub 2}, leading to the formation of regional ozone and other oxidants. Typically, PANs are measured by using a gas chromatograph with electron-capture detection (ECD). Once automated, this method has been shown to be reliable and quite sensitive, allowing the levels of PANs to be measured at low parts per trillion in the troposphere. Unfortunately, a number of other atmospheric gases also have strong ECD signals or act as inferences and limit the speed in which the analysis can be completed. Currently, the shortest analysis time for PAN is approx. 5 minutes with ECD. The authors recent examined the luminol detection of NO{sub 2} and PANs using gas capillary chromatography for rapid monitoring of these important trace gases. Analysis of the PANs (PAN, PPN, and PBN) and NO{sub 2} in one minute has been demonstrated in laboratory studies by using this approach. Reported here are modifications of this instrument for aircraft operation and preliminary results from test flights taken near Pasco, Washington in August of 1997.

  4. Aircraft measurements of nitrogen dioxide and peroxyacetyl nitrates using luminol chemiluminescence with fast capillary gas chromatography

    SciTech Connect

    Gaffney, J.S.; Marley, N.A.; Steele, H.D.; Drayton, P.J.; Hubbe, J.M.

    1999-10-01

    Fast capillary gas chromatography with luminol detection has been used to make airborne measurements of nitrogen dioxide (NO{sub 2}) and peroxyacetyl nitrate (PAN). The analysis system allows for the simultaneous measurement of NO{sub 2} and peroxyacyl nitrates (PANs) with time resolution of less than 1 min, and improvement of a factor of 4--5 over previously reported methods using electron capture detection. Data presented were taken near Pasco, Washington, in August 1997, during a test flight onboard the US Department of Energy G-1 aircraft. The authors report measurements of NO{sub 2} in the boundary layer in a paper mill plume and a plume from a grass fire, in addition to analyses for free tropospheric NO{sub 2} and PAN. Ratios of PAN/NO{sub 2} were observed to increase with altitude (decreasing temperature) and to reach values of 2--4 above the boundary layer, consistent with the thermal equilibrium of the peroxyacetyl radical and NO{sub 2} and PAN. Estimates for the peroxyacetyl radical in the continental free troposphere, calculated from this equilibrium, were found to be in the range of 10{sup 4}--10{sup 5} molecules per cubic centimeter. These results demonstrate the application of this approach for airborne measurements of NO{sub 2} and PAN in a wide range of field study scenarios.

  5. Inhibition of the system luminol-H2O2-Fe(CN)6(3)-chemiluminescence by the Mn(II) indirect determination of isoniazid in a pharmaceutical formulation.

    PubMed

    Gregorio Alapont, A; Aurechia Giménez, E; Lahuerta Zamora, L; Martínez Calatayud, J

    1998-01-01

    A flow injection procedure for the indirect chemiluminescent determination of isoniazid is proposed. The method is performed in a flow-injection manifold provided with a solid-phase reactor. The reactor was made from manganese dioxide physically entrapped by polymerization; the redox reaction isoniazid-manganese dioxide released Mn(II) which was monitored through its inhibitory effect on the reaction between luminol and hydrogen peroxide in presence of potassium hexacyanoferrate (III). The procedure resulted in a linear calibration graph over the range 5-15 mg/L of isoniazid with a sample throughput of 43 samples/h. The influence of foreign compounds was studied and the method was applied to determination of the drug in a pharmaceutical formulation. PMID:9693980

  6. Characterization of the Luminol-Amplified Light Generating Reaction Induced in Human Monocytes

    Microsoft Academic Search

    Agneta Johansson; Claes Dahigren

    Increased productIon of oxidative metabolites following interaction between mononu- clear phagocytes and soluble stimuli can be measured as luminol-amplified chemilumi- nescence (CL). The effects of superoxide dlsmutase (SOD), catalase, and azide on the monocyte CL response were investigated. Azide, a myeloperoxidase (MPO) inhibitor, reduced the CL reaction by more than 80%, which indicates that the CL reaction is dependent on

  7. Measurement of product alignment in beam-gas chemiluminescent reactions

    E-print Network

    Zare, Richard N.

    Measurement of product alignment in beam-gas chemiluminescent reactions Michael G. Prisant, Charles of the chemiluminescent atom-diatom exchange reaction A + BC-.AB* + C under beam-gas conditions. The degree of product momentum appears as product rotational angular momentum. For beam-gas chemiluminescence, this implies

  8. Fast simultaneous determination of traces of Cu(II) and Co(II) in soils and sediments with the luminol/perborate chemiluminescent system.

    PubMed

    Pulgarín, José A Murillo; Bermejo, Luisa F García; Durán, Armando Carrasquero

    2013-01-01

    A flow injection analysis method based on ion chromatography and luminol chemiluminescence detection was used for the simultaneous determination of copper (II) and cobalt (II) trace levels in soils and sediments following microwave-assisted acid digestion. Detection was based on chemiluminescence (CL) of the luminol-perborate system in an alkaline medium, which is catalyzed by both transition metals. The concentration and pH of the eluent (oxalic acid) was found to affect CL intensities and retention times, both of which were inversely proportional to the oxalic acid concentration. The calibration curves for both metal ions were linear and allowed a limit of detection of 0.003 ?g l(-1) for cobalt (II) and 0.014 ?g l(-1) for cooper (II) to be calculated. The proposed method was successfully used to determine both metal ions in certified reference materials of stream and river sediments and soil samples. Based on the results, the determination is free of interferences from the usual concomitant ions. PMID:22392616

  9. Real-time measurement of nitric oxide by luminol-hydrogen peroxide reaction in crystalloid perfused rat heart.

    PubMed

    Tsukada, Yayoi; Yasutake, Masahiro; Jia, Dalin; Kusama, Yoshiki; Kishida, Hiroshi; Takano, Teruo; Tsukada, Shingo

    2003-01-17

    The objective of this study was to develop an assay system that allows continuous monitoring of nitric oxide (NO) released from crystalloid perfused hearts. We utilized chemiluminescence reaction between NO and luminol-H(2)O(2) to quantify the NO level in coronary effluent. Isolated rat hearts were subjected to ordinary Langendorff's perfusion, and the right ventricle was cannulated to sample coronary effluent. After equilibration, the coronary flow rate was set constant and the hearts were paced at 300 bpm. Coronary effluent was continuously sampled and mixed with the chemiluminescent probe containing 0.018 mmol/l luminol plus 10 mmol/l H(2)O(2). Chemiluminescence from the mixture of coronary effluent and the probe was continuously measured. NO concentration was calibrated by various concentrations (0.5-400 pmol/l) of standard NO solution. The lower detection limit of NO was 1 pmol/l. Basal NO release from isolated perfused rat heart was 0.41 +/- 0.17 pmol/min/g of heart weight, and that was significantly suppressed by 0.1 mmol/l of L-NAME to 0.18 +/- 0.10 pmol/min/g of heart weight (n = 7). Application of 0.1 and 0.3 micromol/l acetylcholine increased NO level in the coronary effluent, in a concentration-dependent manner, from 6.6 +/- 1.7 in a baseline condition to 16.3 +/- 7.4 and 30.3 +/- 16.1 pmol/l at each peak, respectively. Thrombin at 1 and 10 U/ml also increased NO level from 17.6 +/- 4.3 in control to 35.5 +/- 10.4 and 48.7 +/- 8.7 pmol/l at each peak, respectively (n = 7). Thus, this assay system is applicable to the continuous real-time measurement of NO released from crystalloid perfused hearts, and it may be useful for the study of physiological or pathophysiological role of NO in coronary circulation. PMID:12495778

  10. Indirect determination of paracetamol in pharmaceutical formulations by inhibition of the system luminol-H2O2-Fe(CN)3-(6) chemiluminescence.

    PubMed

    Alapont, A G; Zamora, L L; Calatayud, J M

    1999-11-01

    After a large drug scanning, the system Luminol-H2O2-Fe(CN)6(3-) is proposed for first time for the indirect determination of paracetamol. The method is based on the oxidation of paracetamol by hexacyanoferrate (III) and the subsequent inhibitory effect on the reaction between luminol and hydrogen peroxide. The procedure resulted in a linear calibration graph over the range 2.5-12.5 microg ml(-1) of paracetamol with a sample throughput of 87 samples h(-1). The influence of foreign compounds was studied and, the method was applied to determination of the drug in three different pharmaceutical formulations. PMID:10703985

  11. An optimized, sensitive and stable reduced graphene oxide-gold nanoparticle-luminol-H2O2 chemiluminescence system and its potential analytical application

    NASA Astrophysics Data System (ADS)

    Wang, Wen-Shuo; He, Da-Wei; Wang, Ji-Hong; Duan, Jia-Hua; Peng, Hong-Shang; Wu, Hong-Peng; Fu, Ming; Wang, Yong-Sheng; Zhang, Xi-Qing

    2014-04-01

    The chemiluminescence (CL) performance of luminol is improved using reduced graphene oxide/gold nanoparticle (rGO-AuNP) nano-composites as catalyst. To prepare this catalyst, we propose a linker free, one-step method to in-situ synthesize rGO-AuNP nano-composites. Various measurements are utilized to characterize the resulting rGO-AuNP samples, and it is revealed that rGO could improve the stability and conductivity. Furthermore, we investigate the CL signals of luminal catalyzed by rGO-AuNP. Afterwards, the size effect of particle and the assisted enhancement effect of rGO are studied and discussed in detail. Based on the discussion, an optimal, sensitive and stable rGO-AuNP-luminon-H2O2 CL system is proposed. Finally, we utilize the system as a sensor to detect hydrogen peroxide and organic compounds containing amino, hydroxyl, or thiol groups. The CL system might provide a more attractive platform for various analytical devices with CL detection in the field of biosensors, bioassays, and immunosensors.

  12. Enhancement of chemiluminescence for vitamin B 12 analysis

    Microsoft Academic Search

    Sagaya Selva Kumar; Raghuraj Singh Chouhan; Munna Singh Thakur

    2009-01-01

    In the current article, chemiluminescence (CL) from the vitamin B12 and luminol reaction was studied under alkaline conditions to develop a sensitive analytical method for vitamin B12 using the carbonate enhancement effect. The method was successfully applied to the determination of vitamin B12 in vitamin B12 tablets, multivitamin capsules, and vitamin B12 injections. Experimental parameters were optimized, including luminol concentration,

  13. Chemiluminescent Westerns: How film and photochemistry

    E-print Network

    Cai, Long

    Chemiluminescent Westerns: How film and photochemistry affect experimental results LI, enhanced chemiluminescence has been used to detect proteins on Western blots.1 Sec- ondary antibodies are labeled with the horseradish peroxidase (HRP) enzyme, which oxidizes the luminol- based chemiluminescent

  14. Surface-mediated chemiluminescent reaction of O and NO

    NASA Astrophysics Data System (ADS)

    Arnold, Graham S.; Coleman, Dianne J.

    1991-02-01

    Deep-red chemiluminescence occurs when beams of O and NO react on metal surfaces. Of the metals studied (Ni, Co, Pt; Kovar, Invar, and Monel), nickel was the most effective at promoting the chemiluminescent reaction. The rate depends inversely on the surface temperature. The reaction rate appears to be controlled by the flux of NO to the surface, and does not depend strongly on the NO beam temperature. These behaviors indicate that the chemiluminescence arises not from a gas-phase reaction near the surface, but from a surface-mediated reaction of O and NO to produce electronically excited gas-phase NO 2, most probably of the Langmuir—Hinshelwood variety.

  15. Determination of phenobarbital in human urine and serum using flow injection chemiluminescence

    Microsoft Academic Search

    X. Li; L. C. Niu; X. L. He; Z. H. Song

    2011-01-01

    A sensitive chemiluminescence method has been proposed for Phenobarbital (PB) determination. It is based on the enhancive\\u000a effect of PB on the chemiluminescence reaction between luminol and dissolved oxygen in a flow injection system. The chemiluminescence\\u000a intensity linearly responded to the PB concentrations ranged from 0.05 to 10 ng\\/ml with the detection limit of 0.02 ng\\/ml\\u000a (3?). At a flow

  16. Determination of Picogram-Levels of Acetylspiramycin in Human Urine and Serum by Flow Injection Chemiluminescence

    Microsoft Academic Search

    Zhenghua Song; Changna Wang

    2005-01-01

    A sensitive chemiluminescence method for the determination of acetylspiramycin is presented. It is based on the greatly enhancive effect of acetylspiramycin on the chemiluminescence reaction between luminol and hydrogen peroxide in the flow system. The increase in chemiluminescence intensity was linearly proportional to the acetylspiramycin concentration in the range from 10?pg?·?mL ?1 to 2.0?ng?·?mL ?1 (r 2?=?0.9979). The detection limit

  17. Enhanced chemiluminescent determination of chloramphenicol and related nitro compounds by 'on-line' photochemical reaction.

    PubMed

    David, V; Saèz, R M; Mateo, J V; Calatayud, J M

    2000-07-01

    The viability of tandem photochemical reaction-chemiluminescence detection was studied for a heterogeneous family of nitro compounds using chloramphenicol as a test substance. The 'on-line' chemical photodegradation of chloramphenicol was performed in a flow injection assembly by using a photoreactor consisting of a 725 cm x 0.5 mm id piece of PTFE tubing coiled around an 8 W low-pressure mercury lamp. Photodegraded chloramphenicol was detected by oxidizing photo-fragments from the parent compound and their subsequent reaction with a luminol-Co(II) system. The calibration graph was linear up to 3 x 10(-5) mol l-1 chloramphenicol, the limit of detection was 3 x 10(-9) mol l-1, the relative standard deviation was 1.8% for 2 x 10(-6) mol l-1 of the drug and the sample throughput was 60 h-1. The proposed method was used to determine chloramphenicol in pharmaceutical formulations and its application to other related nitro compounds was studied. PMID:10984928

  18. Sensitive chemiluminescence method for the determination of glutathione, l -cysteine and 6-mercaptopurine

    Microsoft Academic Search

    Ping Yang; Yonghong Chen; Qiyong Zhu; Fengwu Wang; Lun Wang; Yongxin Li

    2008-01-01

    Glutathione (GSH), l-cysteine (l-Cys) and 6-mercaptopurine (6-MP) inhibit the CL reaction of luminol–H2O2 catalyzed by gold colloids. In order to explore this, GSH, l-Cys and 6-MP were injected into the chemiluminescence system of luminol and H2O2 catalyzed by gold colloids. The results showed that gold colloids interact with GSH, l-Cys and 6-MP and decrease the CL emission. Based on this

  19. Gas-phase chemiluminescent reactions of ozone with monoterpenes

    NASA Astrophysics Data System (ADS)

    Arora, P. K.; Chatha, J. P. S.; Vohra, K. G.

    1983-08-01

    Chemiluminescent reactions of ozone with monoterpenes such as linallol, geraniol, d-limonene and ?-pinene have been studied in the gas phase at low pressures. Methylglyoxal phosphorescence has been observed in the first two reactions. Emissions from HCHO( 1A 2) and glyoxal ( 3A u) are observed in the reaction of ozone with d-limonene and formation of excited glyoxal is found to be first order in ozone. The reaction of ozone with ?-pinene gives rise to emission from a ?-dicarbonyl compound and this is found to be first order in ozone. The mechanisms for the formation of excited species are proposed.

  20. Chemiluminescent imaging of transpired ethanol from the palm for evaluation of alcohol metabolism.

    PubMed

    Arakawa, Takahiro; Kita, Kazutaka; Wang, Xin; Miyajima, Kumiko; Toma, Koji; Mitsubayashi, Kohji

    2015-05-15

    A 2-dimensional imaging system was constructed and applied in measurements of gaseous ethanol emissions from the human palm. This imaging system measures gaseous ethanol concentrations as intensities of chemiluminescence by luminol reaction induced by alcohol oxidase and luminol-hydrogen peroxide-horseradish peroxidase system. Conversions of ethanol distributions and concentrations to 2-dimensional chemiluminescence were conducted on an enzyme-immobilized mesh substrate in a dark box, which contained a luminol solution. In order to visualize ethanol emissions from human palm skin, we developed highly sensitive and selective imaging system for transpired gaseous ethanol at sub ppm-levels. Thus, a mixture of a high-purity luminol solution of luminol sodium salt HG solution instead of standard luminol solution and an enhancer of eosin Y solution was adapted to refine the chemiluminescent intensity of the imaging system, and improved the detection limit to 3ppm gaseous ethanol. The highly sensitive imaging allows us to successfully visualize the emissions dynamics of transdermal gaseous ethanol. The intensity of each site on the palm shows the reflection of ethanol concentrations distributions corresponding to the amount of alcohol metabolized upon consumption. This imaging system is significant and useful for the assessment of ethanol measurement of the palmar skin. PMID:25445620

  1. Flow-injection chemiluminescence determination of chlorinated isocyanuric acids.

    PubMed

    Safavi, Afsaneh; Karimi, Mohammad Ali

    2003-02-01

    A rapid and sensitive flow-injection chemiluminescence method is described for the determination of dichloro- and trichloroisocyanuric acids based on the chemiluminescence produced during their reaction with luminol in alkaline medium. The effects of analytical and flow-injection variables on these chemiluminescence systems and determination of both oxidants are discussed. The optimized method yielded 3sigma detection limits of 8x10(-8) and 5x10(-8) mol L(-1) for the sodium dichloroisocyanurate and trichloroisocyanuric acid, respectively. The optimum conditions were found to be as follows: NaOH, 1x10(-1) mol L(-1); luminol, 5x10(-3) mol L(-1); KI, 2x10(-3) mol L(-1) and flow rate, 3.5 mL min(-1). PMID:12589508

  2. Chemiluminescence resonance energy transfer imaging on magnetic particles for single-nucleotide polymorphism detection based on ligation chain reaction.

    PubMed

    Bi, Sai; Zhang, Zhipeng; Dong, Ying; Wang, Zonghua

    2014-10-17

    A novel ligation chain reaction (LCR) methodology for single-nucleotide polymorphism (SNP) detection was developed based on luminol-H2O2-horseradish peroxidase (HRP)-mimicking DNAzyme-fluorescein chemiluminescence resonance energy transfer (CRET) imaging on magnetic particles. For LCR, four unique target-complement probes (X and X(?), YG and Y(?)) for the amplification of K-ras (G12C) were designed by modifying G-quadruplex sequence at 3'-end of YG and fluorescein at 5'-end of Y(?). After the LCR, the resulting products of XYG/X(?)Y(?) with biotin-labeled X(?) were captured onto streptavidin-coated magnetic particles (SA-MPs) via specific biotin-SA interaction, which stimulated the CRET reaction from hemin/G-quadruplex-catalyzed luminol-H2O2 CL system to fluorescein. By collecting signals by a cooled low-light CCD, a CRET imaging method was proposed for visual detection and quantitative analysis of SNP. As low as 0.86fM mutant DNA was detected by this assay, and positive mutation detection was achieved with a wild-type to mutant ratio of 10,000:1. This high sensitivity and specificity could be attributed to not only the exponential amplification and excellent discrimination of LCR but also the employment of SA-MPs. SA-MPs ensured the feasibility of the proposed strategy, which also simplified the operations through magnetic separation and separated the reaction and detection procedures to improve sensitivity. The proposed LCR-CRET imaging strategy extends the application of signal amplification techniques to SNP detection, providing a promising platform for effective and high-throughput genetic diagnosis. PMID:25461149

  3. Determination of Antioxidant Activity of Canary Seed Infusions by Chemiluminescence 1

    Microsoft Academic Search

    M. J. Novas; A. M. Jiménez; A. G. Asuero

    2004-01-01

    The antioxidant activity of canary seed or annual canary grass (Phalaris canariensis L.) infusions was demonstrated by the influence of these over the chemiluminescent emission of a luminol reaction in an oxidizing medium (hydrogen peroxide). In order to quantify this activity, the antioxidant capacity of a canary seed infusion was compared with a potent antioxidant such as ascorbic acid.

  4. Organ Chemiluminescence: Noninvasive Assay for Oxidative Radical Reactions

    Microsoft Academic Search

    Alberto Boveris; Enrique Cadenas; Rudolf Reiter; Mark Filipkowski; Yuzo Nakase; Britton Chance

    1980-01-01

    In situ and perfused rat livers showed a spontaneous chemiluminescence of 7-12 counts \\/ sec\\\\cdot cm2 (corresponding to 7-12× 103 photons \\/ sec\\\\cdot cm2); chemiluminescence was increased up to 30 times by infusion of exogenous hydroperoxides. The chemiluminescence of the perfused liver was oxygen dependent. Ethyl, t-butyl, and cumene hydroperoxides were almost equally effective in inducing light emission in the

  5. Detection of gamma irradiated pepper and papain by chemiluminescence

    NASA Astrophysics Data System (ADS)

    Sattar, Abdus; Delincée, H.; Diehl, J. F.

    Chemiluminescence (CL) measurements of black pepper and of papain using luminol and lucigenin reactions were studied. Effects of grinding, irradiation (5-20 kGy) and particle size (750-140 ?m) on CL of pepper, and of irradiation (10-30 kGy) on CL of papain, were investigated. All the tested treatments affected the luminescence response in both the luminol and lucigenin reactions; however, the pattern of changes in each case, was inconsistent. Optimum pepper size for maximum luminescence was 560 ?m, and optimum irradiation doses were >15 kGy for pepper and >20 kGy for papain. Chemiluminescence may possibly be used as an indicator or irradiation treatment for pepper and papain at a dose of 10 kGy or higher, but further research is needed to establish the reliability of this method.

  6. Rapid determination of levofloxacin in pharmaceuticals and biological fluids using a new chemiluminescence system

    Microsoft Academic Search

    Xiaodong Shao; Ying Li; Yangqin Liu; Zhenghua Song

    2011-01-01

    A novel chemiluminescence method for the determination of levofloxacin is presented, which is based on the inhibitory effect\\u000a of levofloxacin on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system. The decrement\\u000a of chemiluminescence intensity is linear with the logarithm of levofloxacin concentration over the range from 0.07 to 100.0\\u000a ng\\/mL (r\\u000a 2 = 0.9994), with the detection

  7. Recent applications of electrogenerated chemiluminescence in chemical analysis

    Microsoft Academic Search

    Karsten A Fähnrich; Miloslav Pravda; George G Guilbault

    2001-01-01

    Analytical applications of electrogenerated chemiluminescence (ECL) are reviewed with emphasis on the years 1997–2000. Recent developments are described for the ECL of organics, metal complexes and clusters, cathodic ECL on oxide covered electrodes, ECL based immunosensors, DNA-probe assays and enzymatic biosensors. Mechanisms are given for polyaromatic hydrocarbons, luminol\\/hydrogen peroxide, some cathodic ECL reactions and ruthenium complexes with and without co-reactants.

  8. In vitro generation of reactive oxygen species by free coelomic cells of the annelid Eisenia fetida andrer: An analysis by chemiluminescence and nitro blue tetrazolium reduction

    Microsoft Academic Search

    Pierre Valembois; Maguy Lassègues

    1995-01-01

    Coelomocytes of the earthworm Eisenia fetida andrei were activated in vitro with various stimulants in order to investigate their capacity to produce reactive oxygen species. Analysis by luminol-enhanced chemiluminescence and nitro blue tetrazolium reduction suggests the production in vitro of reactive oxygen species by both categories of free coelomocytes, leucocytes and chloragocytes, while affecting different modalities: a respiratory burst-like reaction

  9. Development of a chemiluminescent and bioluminescent system for the detection of bacteria in wastewater effluent

    NASA Technical Reports Server (NTRS)

    Thomas, R. R.

    1975-01-01

    Automated chemiluminescent and bioluminescent sensors were developed for continuous monitoring of microbial levels in wastewater effluent. Development of the chemiluminescent system included optimization of reagent concentrations as well as two new techniques which will allow for increased sensitivity and specificity. The optimal reagent concentrations are 0.0025 M luminol and 0.0125 M sodium perborate in 0.75N sodium hydroxide before addition of sample. The methods developed to increase specificity include (1) extraction of porphyrins from bacteria collected in a filter using 0.1N NaOH - 50 percent Ethanol, and (2) use of the specific reaction rate characteristics for the different luminol catalysts. Since reaction times are different for each catalyst, the reaction can be made specific for bacteria by measuring only the light emission from the particular reaction time zone specific for bacteria. Developments of the bioluminescent firefly luciferase system were in the area of flow system design.

  10. Infrared chemiluminescence study of the reaction Cl + HI yielding HCl + I at enhanced collision energies.

    NASA Technical Reports Server (NTRS)

    Cowley, L. T.; Horne, D. S.; Polanyi, J. C.

    1971-01-01

    Performed chemiluminescence and beam experiments show a markedly increased efficiency of conversion of the reaction energy into vibration and a markedly enhanced tendency for forward scattering in the reaction Cl + HI yields HCl + I as compared with H + Cl2 yields HCl + Cl. These differences appear to be due predominantly to the difference in the masses involved.

  11. [Oxidation of luminol with peroxidase from royal palm leaves].

    PubMed

    Alpeeva, I S; Sakharov, I Iu

    2007-01-01

    We optimized the conditions for luminol oxidation by hydrogen peroxide in the presence of peroxidase (EC 1.11.1.7) from royal palm leaves (Roystonea regia). The pH range (8.3-8.6) corresponding to maximum chemiluminescence was similar for palm tree peroxidase and horseradish peroxidase. Variations in the concentration of the Tris buffer were accompanied by changes in chemiluminescence. Note that maximum chemiluminescence was observed in the 30 mM solution. The detection limit of the enzyme assay during luminol oxidation by hydrogen peroxide was 1 pM. The specific feature of palm tree peroxidase was the generation of a long-term chemiluminescent signal. In combination with the data on the high stability of palm tree peroxidase, our results indicate that this enzyme is promising for its use in analytical studies. PMID:17345855

  12. A direct interaction model for chemiluminescent reactions M. G. Prisant,a) C. T. Rettner,b) and R. N. ZareC

    E-print Network

    Zare, Richard N.

    A direct interaction model for chemiluminescent reactions M. G. Prisant,a) C. T. Rettner,b) and R-gas chemiluminescence reactions of the type: A + BC-+AB* + C, where AB* is an electronically excited diatomic product. For reactions studied using the technique ofelectronic chemiluminescence, the theoretical approach

  13. Enhanced chemiluminescence: a sensitive analytical system for detection of sweet potato peroxidase.

    PubMed

    Vdovenko, Marina M; Della Ciana, Leopoldo; Sakharov, Ivan Y

    2010-08-01

    3-(10'-Phenothiazinyl)propane-1-sulfonate (SPTZ) was shown to be a potent enhancer of anionic sweet potato peroxidase (aSPP)-induced chemiluminescence. The optimal conditions for aSPP-catalyzed oxidation of luminol were investigated by varying the concentrations of luminol, hydrogen peroxide, Tris, and SPTZ as well as the pH values of the reaction mixture. Addition of 4-morpholinopyridine (MORP) to the reaction mixture markedly increased the light intensity. Using SPTZ and MORP together enhanced the effect 265 times. The lower detection limit (LDL) of SPP was 0.09 pM, approximately in 10 times lower than that for the cationic isozyme c of horseradish peroxidase/4-iodophenol system. It was shown that aSPP in the presence of SPTZ produced a longer lasting chemiluminescent signal. PMID:20449843

  14. Determination of Cytochrome c in Human Serum and Pharmaceutical Injections Using Flow Injection Chemiluminescence

    Microsoft Academic Search

    Xin Li; Houyong Liu; Xili He; Zhenghua Song

    2010-01-01

    It was found that the complex of cytochrome c (Cyt c) and hydrogen peroxide could significantly catalyze the chemiluminescence (CL) reaction from luminol–hydrogen peroxide, and\\u000a a sensitive, rapid, and simple CL procedure was proposed for the determination of Cyt c in a flow injection system for the first time. The increment of CL intensity was linear over the concentration of

  15. Chemiluminescence assay for quinones based on generation of reactive oxygen species through the redox cycle of quinone.

    PubMed

    Kishikawa, Naoya; Ohkubo, Nobuhiro; Ohyama, Kaname; Nakashima, Kenichiro; Kuroda, Naotaka

    2009-02-01

    A sensitive and selective chemiluminescence assay for the determination of quinones was developed. The method was based on generation of reactive oxygen species through the redox reaction between quinone and dithiothreitol as reductant, and then the generated reactive oxygen was detected by luminol chemiluminescence. The chemiluminescence was intense, long-lived, and proportional to quinone concentration. It is concluded that superoxide anion was involved in the proposed chemiluminescence reaction because the chemiluminescence intensity was decreased only in the presence of superoxide dismutase. Among the tested quinones, the chemiluminescence was observed from 9,10-phenanthrenequinone, 1,2-naphthoquinone, and 1,4-naphthoquinone, whereas it was not observed from 9,10-anthraquinone and 1,4-benzoquinone. The chemiluminescence property was greatly different according to the structure of quinones. The chemiluminescence was also observed for biologically important quinones such as ubiquinone. Therefore, a simple and rapid assay for ubiquinone in pharmaceutical preparation was developed based on the proposed chemiluminescence reaction. The detection limit (blank + 3SD) of ubiquinone was 0.05 microM (9 ng/assay) with an analysis time of 30 s per sample. The developed assay allowed the direct determination of ubiquinone in pharmaceutical preparation without any purification procedure. PMID:19066858

  16. Multicommuted flow system for the determination of glucose in animal blood serum exploiting enzymatic reaction and chemiluminescence detection

    PubMed Central

    Pires, Cherrine K.; Martelli, Patrícia B.; Lima, José L. F. C.; Saraiva, Maria Lúcia M. F. S.

    2003-01-01

    An automatic flow procedure based on multicommutation dedicated for the determination of glucose in animal blood serum using glucose oxidase with chemiluminescence detection is described. The flow manifold consisted of a set of three-way solenoid valves assembled to implement multicommutation. A microcomputer furnished with an electronic interface and software written in Quick BASIC 4.5 controlled the manifold and performed data acquisition. Glucose oxidase was immobilized on porous silica beads (glass aminopropyl) and packed in a minicolumn (15 × 5 mm). The procedure was based on the enzymatic degradation of glucose, producing hydrogen peroxide, which oxidized luminol in the presence of hexacyanoferrate(III), causing the chemiluminescence. The system was tested by analysing a set of serum animal samples without previous treatment. Results were in agreement with those obtained with the conventional method (LABTEST Kit) at the 95% confidence level. The detection limit and variation coefficient were estimated as 12.0 mg l?1 (99.7% confidence level) and 3.5% (n = 20), respectively. The sampling rate was about 60 determinations h?1 with sample concentrations ranging from 50 to 600 mg l?1 glucose. The consumptions of serum sample, hexacyanoferrate(III) and luminol were 46 ?l, 10.0 mg and 0.2 mg/determination, respectively. PMID:18924619

  17. Short-Time-Response measurements of nitrogen dioxide and peroxyacetyl nitrate by fast capillary gas chromatography with luminol detection.

    SciTech Connect

    Marley, N. A.; Gaffney, J. S.; Drayton, P. J.

    2000-12-07

    The interaction of hydrocarbons and nitrogen oxides in sunlight to produce photochemical smog has been well studied over the years. In the past, the workhorse for the measurement of NO{sub 2}and NO was the chemiluminescent reaction with ozone. This method has detection limits of approximately 0.5 ppb in most commercial instruments, but it cannot detect NO{sub 2} directly; the instrument detects NO and uses hot catalytic surfaces to decompose all other nitrogen oxides (including NO{sub 2}) to NO for detection (l). The main problem with the method is the inherent difficulty in detecting excited NO{sub 2}, which emits over a broad region beginning at approximately 660 nm and has a maximum at 1270 nm, thus requiring a red-shifted photomultiplier for detection. The use of luminol for direct chemiluminescent detection of NO{sub 2} was demonstrated to have greater inherent sensitivity (detection limits of 5 ppt) than the indirect ozone chemiluminescence detection (2). In the luminol system, a gas-liquid reaction leads to light emission with a maximum at approximately 425 nm, at the maximum sensitivity for most photomultiplier tubes. This emission is responsible for the increased detection sensitivities. The biggest problem with this method for direct measurement of NO{sub 2} has been interference due to other soluble oxidants, particularly peroxyacyl nitrates (PANs).

  18. FI-on line photochemical reaction for direct chemiluminescence determination of photodegradated chloramphenicol.

    PubMed

    Icardo, M Catalá; Misiewicz, M; Ciucu, A; García Mateo, J V; Calatayud, J Martínez

    2003-06-13

    A new, simple, clean and selective flow injection strategy based on the tandem photochemical reaction-chemiluminescence detection was applied to the determination of chloramphenicol. The determination is based on the on-line photodegradation of the drug in a glycine buffer at pH 8.8 by using a photoreactor consisting of 697 cmx0.5 mm PTFE tubing helically coiled around an 8 W low-pressure mercury lamp. Photodegradated chloramphenicol is detected by direct chemiluminescence of resulting photo-fragments and their subsequent reaction with potassium permanganate in sulfuric acid medium as oxidant. The method allows the chemiluminescence determination of compounds which do not exhibit native chemiluminescence. The calibration graph was linear up to 14 mug ml(-1) chloramphenicol, the limit of detection was 30 ng ml(-1), the relative standard deviation was 2.4% for 7 mug ml(-1) of the drug and the sample throughput was 60 h(-1). Taking into account the importance of the medium of photodegradation on the mechanism of photodegradation a comparative study in terms of selective was performed for different chemical media employed in the procedure of photodegradation. The proposed method was applied to the determination of chloramphenicol in commercially available pharmaceutical formulations. PMID:18969062

  19. Chemiluminescence spectra of the reaction products of gallium, indium and thallium vapors with nitrous oxide

    SciTech Connect

    Eliseev, M.V.; Koryazhkin, V.A.; Mal'tsev, A.A.; Popov, A.D.

    1983-03-01

    The search for active media for chemical lasers generating in the visible range has led to numerous investigations of the chemiluminescence of oxidation reactions of metals in the gas phase. In the present work, the chemiluminescence spectra of flames of gallium, indium, and thallium vapors in nitrous oxide in an argon flux are investigated. The chemiluminescence intensity was studied as a function of the total pressure in the reactor, the rate of admission of the nitrous oxide, the rate of admission of argon and the cell temperature. The oxide molecules formed are in vibrational levels of the electronic ground state that are close to the dissociational limit. As a result of collisions with argon atoms, the oxide molecule passes to the excited electronic state. The thermal effects of the above reaction and the equal dissociational energies of the oxide molecules are sufficient for excitation of the vibrational levels 10 and 2 of the excited electronic states of the GaO and InO molecules, respectively. Atomic chemiluminescence is evidently a consequence of collision of metal oxide molecules with metal atoms in the electronic ground states.

  20. Reaction of uranium (IV) with xenon difluoride by chemiluminescence, spectrophotometric, and spectrofluorimetric methods

    SciTech Connect

    Mamykin, A.V.; Kazakov, V.P.

    1988-07-01

    A study is made of the kinetics of the chemiluminescent reaction of oxidation of uranium (IV) by xenon difluoride in 1M HClO/sub 4/ U/sup 4 +/ + XeF/sub 2/ ..-->.. UO/sub 2//sup 2 +/ = h/eta/. The optical density D and the intensity of the photoluminescence of the solution I/sub PL/ were measured in parallel with recording of the luminescence intensity I/sub CL/. I/sub CL/ attains a maximum value some time after the beginning of the reaction, after which it decays exponentially. On the kinetic curves of the time dependence of D and I/sub PL/ an induction period is observed, the extent of which depends on concentrations of reagents and temperature of the solution. The maximum of I/sub CL/ coincides with the end of the induction period if the measurements are carried out under identical conditions. The rate of the reaction after the induction period is described by a first order equation in U/sup (IV)/. The rate constants of the reaction, obtained by chemiluminescence, spectrophotometric, and spectrofluorimetric methods, have close values 2.0 +- 0.4, 1.8 +- 0.3, and 2.1 +- 0.3 sec/sup /minus/1/ x 10/sup 2/, respectively. On the basis of the results obtained, we conclude that the stages of formation of UO/sub 2//sup 2 +/ and of chemiluminescence coincide, i.e., formation of the excited state (UD/sub 2//sup 2 +/) and of chemiluminescence coincide, i.e., formation of the excited state (UD/sub 2//sup 2 +/)* takes place during the reaction. It is proposed and experimentally verified that the reaction passes through an intermediate stage of formation of uranyl ion UO/sub 2//sup +/.

  1. Subpicogram determination of Vitamin B 12 in pharmaceuticals and human serum using flow injection with chemiluminescence detection

    Microsoft Academic Search

    Zhenghua Song; Shuang Hou

    2003-01-01

    A sensitive chemiluminescence (CL) method, based on the enhancive effect of cobalt(II) on the CL reaction between luminol and dissolved oxygen in a flow injection (FI) system, was proposed for determination of Vitamin B12. The increment of the CL intensity was proportional to the concentration of Vitamin B12, giving a calibration graph linear over the concentration from 2.0×10?10 to 1.2×10?6gl?1

  2. Mechanism of action of 4-dialkylaminopyridines as secondary enhancers in enhanced chemiluminescence reaction.

    PubMed

    Sakharov, Ivan Yu; Vdovenko, Marina M

    2013-03-01

    Kinetic study of peroxidase-catalyzed oxidation of 3-(10'-phenothiazinyl)propane-1-sulfonate (SPTZ) by hydrogen peroxide demonstrated that the addition of 4-dialkylaminopyridines to a substrate solution significantly increased a rate of production of SPTZ cation radical and did not affect the rate of decomposition of this radical. These results explain the mechanism of action of 4-dialkylaminopyridines as secondary enhancers in enhanced chemiluminescence reaction used widely in analytical practice. PMID:23123428

  3. Rapid determination of subnanogram urapidil using flow injection enhancement chemiluminescence

    Microsoft Academic Search

    Q. Yue; Z. Song; C. Wang

    2006-01-01

    A sensitive flow-injection (FI) chemiluminescence (CL) for the determination of urapidil is described in this paper. It is\\u000a based on the enhancement effect of urapidil on the CL reaction between luminol and hydrogen peroxide. The increment of CL\\u000a intensity is proportional to the concentration of urapidil in the range 0.1?10 ng\\/mL (R\\u000a 2=0.9986), with a detection limit (3?) of 0.03

  4. Determination of bisphenol A using a flow injection inhibitory chemiluminescence method.

    PubMed

    Wang, Shuhao; Wei, Xinting; Du, Lingyun; Zhuang, Huisheng

    2005-01-01

    A new flow injection chemiluminescence (CL) method has been developed for the determination of bisphenol A (BPA), based on the inhibitory effect of BPA on the chemiluminescence reaction between luminol and potassium hexacyanoferrate. Under optimum conditions, the decrease in CL emission intensity was linear with BPA concentration in the range 8.0 x 10(-7)-1.2 x 10(-5) mol/L, and the detection limit was 3.1 x 10(-7) mol/L. The relative standard deviation (RSD) of 11 replicate measurements was 2.6% for 2.0 x 10(-6) mol/L BPA (n = 11). The sampling frequency was calculated to be ca. 120/h. This method has been successfully used to determine the content of BPA in aqueous solution of polycarbonate materials. A brief discussion on the possible chemiluminescence reaction mechanism is presented. PMID:15685659

  5. Generation of chemiluminescence upon reaction of aliphatic amines with tris(2,2'-bipyridine)ruthenium(III)

    Microsoft Academic Search

    James B. Noffsinger; Neil D. Danielson

    1987-01-01

    Tris(2,2'-bipyridine)ruthenium(III) (Ru(bpy)â\\/sup 3 +\\/) will undergo an electron-transfer reaction with an appropriate reducing agent to form Ru(bpy)â\\/sup 2 +\\/, which upon achieving an excited state can result in chemiluminescence. Aliphatic amines with an increasing number of carbon atoms were tested between a pH of 4 and 6 and found to act as chemiluminescent reducing agents. In addition, some diamines and

  6. Two techniques for eliminating luminol interference material and flow system configurations for luminol and firefly luciferase systems

    NASA Technical Reports Server (NTRS)

    Thomas, R. R.

    1976-01-01

    Two methods for eliminating luminol interference materials are described. One method eliminates interference from organic material by pre-reacting a sample with dilute hydrogen peroxide. The reaction rate resolution method for eliminating inorganic forms of interference is also described. The combination of the two methods makes the luminol system more specific for bacteria. Flow system designs for both the firefly luciferase and luminol bacteria detection systems are described. The firefly luciferase flow system incorporating nitric acid extraction and optimal dilutions has a functional sensitivity of 3 x 100,000 E. coli/ml. The luminol flow system incorporates the hydrogen peroxide pretreatment and the reaction rate resolution techniques for eliminating interference. The functional sensitivity of the luminol flow system is 1 x 10,000 E. coli/ml.

  7. A new chemiluminescence method for the determination of nickel ion

    NASA Astrophysics Data System (ADS)

    Li, Li Na; Li, Nian Bing; Luo, Hong Qun

    2006-05-01

    A new chemiluminescence (CL) phenomenon described as the second-chemiluminescence (SCL) was observed and a strong CL signal was detected, when Ni(II) ion was injected into the mixture after the end of the reaction of potassium permanganate with alkaline luminol. The possible CL mechanism is proposed based on the kinetic curve of the CL reaction, CL spectra, UV-vis spectra and some other experiments. A flow-injection analysis for the determination of nickle(II) ion has been developed, based on the catalysis of nickel(II) ion on the CL reaction between potassium manganate produced on-line and luminol under alkaline condition. Under the optimum conditions, the SCL intensity is linear with the concentration of nickel(II) ion in the range of 8.0-200.0 ?g l -1 and 0.2-2.0 mg l -1. The R.S.D. was 4.5% for 11 determinations of 250 ?g l -1 nickel(II) ion and the detection limit (3 ?) for nickel(II) ion was 0.33 ?g l -1. The method was applied to determine nickel(II) ion in synthetic samples with satisfactory results.

  8. Energy Transfer Processes of Chemiluminescence Reaction Systems with Cerium(IV) Ions and Their Analytical Application: A Review.

    PubMed

    Kaczmarek, Ma?gorzata

    2015-03-01

    This review is devoted to a thorough discussion of chemiluminescence of the systems containing Ce(IV) ions as oxidising agents, with particular emphasis on the energy transfer processes in such systems. The influence of sensitisers such as: rhodamines, quinine, lanthanide ions and their complexes and quantum-dots has been analysed and the practical use of reaction systems for development of new chemiluminescence methods for determination of therapeutic drugs and substances of biological importance in different matrices such as human urine or serum is indicated. The types of emitters and excited reaction products taking part in energy transfer to sensitisers and processes taking place in the chemiluminescence reaction systems containing Ce(IV) ions are presented on the basis of recent literature. PMID:25656069

  9. Chemiluminescence analysis

    SciTech Connect

    Grayeski, M.L.

    1987-11-01

    Chemiluminescence (CL) is observed when light is emitted from a chemical reaction. If the reaction occurs in a living system or is derived from one, the process is called bioluminescence (BL). Many articles describe clinical, biological, and environmental applications using both gas- and solution-phase CL reactions. Although the lack of commercially available instrumentation, reagents, and methodology has somewhat restricted the widespread use of CL, certain applications are common: the measurement of total microbial cell counts using the firefly reaction and the determination of oxides of nitrogen with a gas-phase chemiluminescent reaction involving ozone. This REPORT will discuss only a few of the most recent developments in CL using reactions in solution.

  10. Chemiluminescence: An Illuminating Experiment

    ERIC Educational Resources Information Center

    Gafney, Harry D.; Adamson, Arthur W.

    1975-01-01

    Describes an experiment in which luminescence is observed during a reaction between sodium borohydride and trisbipyridalruthenium (III). Includes a discussion of the theory of chemiluminescence. (MLH)

  11. Flow-injection chemiluminescence method to detect a ?2 adrenergic agonist.

    PubMed

    Zhang, Guangbin; Tang, Yuhai; Shang, Jian; Wang, Zhongcheng; Yu, Hua; Du, Wei; Fu, Qiang

    2015-02-01

    A new method for the detection of ?2 adrenergic agonists was developed based on the chemiluminescence (CL) reaction of ?2 adrenergic agonist with potassium ferricyanide-luminol CL. The effect of ?2 adrenergic agonists including isoprenaline hydrochloride, salbutamol sulfate, terbutaline sulfate and ractopamine on the CL intensity of potassium ferricyanide-luminol was discovered. Detection of the ?2 adrenergic agonist was carried out in a flow system. Using uniform design experimentation, the influence factors of CL were optimized. The optimal experimental conditions were 1?mmol/L of potassium ferricyanide, 10?µmol/L of luminol, 1.2?mmol/L of sodium hydroxide, a flow speed of 2.6?mL/min and a distance of 1.2?cm from 'Y2 ' to the flow cell. The linear ranges and limit of detection were 10-100 and 5?ng/mL for isoprenaline hydrochloride, 20-100 and 5?ng/mL for salbutamol sulfate, 8-200 and 1?ng/mL for terbutaline sulfate, 20-100 and 4?ng/mL for ractopamine, respectively. The proposed method allowed 200 injections/h with excellent repeatability and precision. It was successfully applied to the determination of three ?2 adrenergic agonists in commercial pharmaceutical formulations with recoveries in the range of 96.8-98.5%. The possible CL reaction mechanism of potassium ferricyanide-luminol-?2 adrenergic agonist was discussed from the UV/vis spectra. PMID:24830367

  12. Isoprinosine stimulates granulocyte chemiluminescence and inhibits monocyte chemiluminescence in vitro.

    PubMed

    Flø, R W; Naess, A; Albrektsen, G; Solberg, C O

    1994-04-01

    Isoprinosine may delay disease progression in human immunodeficiency virus infection, presumably through modulation of lymphocyte function. However, the influence of isoprinosine on phagocyte function is largely unknown. This study describes the effects of isoprinosine and azidothymidine on phagocyte chemiluminescence and migration. Incubation with isoprinosine concentrations of 250 micrograms/ml and above increased the chemiluminescence of granulocytes. Random migration of granulocytes was decreased at isoprinosine concentrations of 50 micrograms/ml and higher, but chemotaxis was not affected. Azidothymidine exerted no effect on the chemiluminescence or migration of granulocytes. For monocytes, luminol-enhanced chemiluminescence was reduced at isoprinosine concentrations of 250 micrograms/ml and above, whereas migration was not affected. These findings suggest that the immunomodulatory properties of isoprinosine may extend to phagocytic cells. This may be of significance in the treatment of immunodeficiency states. PMID:7516672

  13. A global reaction model for oh* chemiluminescence applied to a laminar flat-flame burner

    Microsoft Academic Search

    L. C. Haber; U. Vandsburger

    2003-01-01

    Combustion diagnostics in practical devices are difficult due to the harsh environment encountered. Many optical diagnostics are attractive for combustion diagnostics applications due to their high temperature compatibility. Chemiluminescence measurements may allow combustion monitoring at a level of detail traditionally thought impossible. Among chemiluminescence species observed in flames, OH* chemiluminescence is attractive due to its relatively strong emission at lean

  14. Chemiluminescence determination of gemifloxacin based on diperiodatoargentate (III)-sulphuric acid reaction in a micellar medium.

    PubMed

    Zhao, Fang; Zhao, Wen-hui; Xiong, Wei

    2013-01-01

    A novel flow-injection chemiluminescence (FI-CL) analysis method for the determination of gemifloxacin in the presence of cetyltrimethylammonium bromide (CTAB) surfactant micelles is described. Strong CL signal was generated during the reaction of gemifloxacin with diperiodatoargentate (III) in a sulfuric acid medium sensitized by CTAB. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of gemifloxacin from 1.0 × 10(-9) to 3.0 × 10(-7) g/mL and the detection limit was 7.3 × 10(-10) g/mL (3?). The relative standard deviation (RSD) was 1.7 % for a 3.0 × 10(-8) g/mL gemifloxacin solution (11 repeated measurements). The proposed method was successfully applied to the determination of gemifloxacin in pharmaceutical preparations and biological fluids. The possible mechanism of the CL reaction is also discussed briefly. PMID:22362634

  15. Method and apparatus for eliminating luminol interference material

    NASA Technical Reports Server (NTRS)

    Jeffers, E. L.; Thomas, R. R. (inventors)

    1979-01-01

    A method and apparatus for removing porphyrins from a fluid sample which are unrelated to the number of bacteria present in the sample and prior to combining the sample with luminol reagent to produce a light reaction is disclosed. The method involves a pre-incubation of the sample with a dilute concentration of hydrogen peroxide which inactivates the interfering soluble porphyrins. Further, by delaying taking a light measurement for a predetermined time period after combining the hydrogen peroxide-treated water sample with a luminol reagent, the luminescence produced by the reaction of the luminol reagent with ions present in the solution, being short lived, will have died out so that only porphyrins within the bacteria which have been released by rupturing the cells with the sodium hydroxide in the luminol reagent, will be measured. The measurement thus obtained can then be related to the concentration of live and dead bacteria in the fluid sample.

  16. Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis

    PubMed Central

    Wang, Jiajia; Tan, Xijuan; Song, Zhenghua

    2013-01-01

    Based on the inhibitory effect of uric acid (UA) on luminol-Co2+ chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24?h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742?mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2?h after meals with the values of 417, 318, and 288??g?mL?1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA?CL reaction was discussed in detail. PMID:24251067

  17. Chemiluminescence as diagnostic tool. A review

    Microsoft Academic Search

    C. Dodeigne; L. Thunus; R. Lejeune

    2000-01-01

    The principles of chemiluminescence and its applications as diagnostic tool are reviewed. After an introduction to the theoretical aspects of luminescence and energy transfer, the different classes of chemiluminogenic labels including luminol, acridinium compounds, coelenterazine and analogues, dioxetanes, systems based on peroxyoxalic acid and their derivatives are described emphasizing the molecules which best fulfil the requirements of today’s clinical chemistry.

  18. Direct-injection chemiluminescence detector. Properties and potential applications in flow analysis.

    PubMed

    Koronkiewicz, Stanislawa; Kalinowski, Slawomir

    2015-02-01

    We present a novel chemiluminescence detector, with a cone-shaped detection chamber where the analytical reaction takes place. The sample and appropriate reagents are injected directly into the chamber in countercurrent using solenoid-operated pulse micro-pumps. The proposed detector allows for fast measurement of the chemiluminescence signal in stop-flow conditions from the moment of reagents mixing. To evaluate potential applications of the detector the Fenton-like reaction with a luminol-H2O2 system and several transition metal ions (Co(2+), Cu(2+), Cr(3+), Fe(3+)) as a catalyst were investigated. The results demonstrate suitability of the proposed detector for quantitative analysis and for investigations of reaction kinetics, particularly rapid reactions. A multi-pumping flow system was designed and optimized. The developed methodology demonstrated that the shape of the analytical signals strongly depends on the type and concentration of the metal ions. The application of the detector in quantitative analysis was assessed for determination of Fe(III). The direct-injection chemiluminescence detector allows for a sensitive and repeatable (R.S.D. 2%) determination. The intensity of chemiluminescence increased linearly in the range from about 0.5 to 10 mg L(-1) Fe(III) with the detection limit of 0.025 mg L(-1). The time of analysis depended mainly on reaction kinetics. It is possible to achieve the high sampling rate of 144 samples per hour. PMID:25435236

  19. A novel chemiluminescence paper microfluidic biosensor based on enzymatic reaction for uric acid determination.

    PubMed

    Yu, Jinghua; Wang, Shoumei; Ge, Lei; Ge, Shenguang

    2011-03-15

    In this work, chemiluminescence (CL) method was combined with microfluidic paper-based analytical device (?PAD) to establish a novel CL ?PAD biosensor for the first time. This novel CL ?PAD biosensor was based on enzyme reaction which produced H(2)O(2) while decomposing the substrate and the CL reaction between rhodanine derivative and generated H(2)O(2) in acid medium. Microchannels in ?PAD were fabricated by cutting method. And the possible CL assay principle of this CL ?PAD biosensor was explained. Rhodanine derivative system was used to reach the purpose of high sensitivity and well-defined signal for this CL ?PAD biosensor. And the optimum reaction conditions were investigated. The quantitative determination of uric acid could be achieved by this CL ?PAD biosensor with accurate and satisfactory result. And this biosensor could provide good reproducible results upon storage at 4°C for at least 10 weeks. The successful integration of ?PAD and CL reaction made the final biosensor inexpensive, easy-to-use, low-volume, and portable for uric acid determination, which also greatly reduces the cost and increases the efficiency required for an analysis. We believe this simple, practical CL ?PAD biosensor will be of interest for use in areas such as disease diagnosis. PMID:21257303

  20. Synchronization of oscillatory chemiluminescence with pulsed light irradiation

    NASA Astrophysics Data System (ADS)

    Takayama, Shunsuke; Okano, Kunihiko; Asakura, Kouichi

    2013-01-01

    A chemical oscillator, the H2O2-KSCN-CuSO4-NaOH system, generates an oscillatory chemiluminescence when luminol is added to this system. Attempts were made to synchronize the oscillatory chemiluminescence with pulsed light irradiation. A period of the chemical oscillation became shorter by the irradiation of white and blue color light, while the oscillatory behavior was scarcely influenced by the irradiation of red light. Pulsed red and white or blue lights were irradiated on either the non-luminol or luminol-added H2O2-KSCN-CuSO4-NaOH system. Synchronization of the chemical oscillation was achieved for 25-30 min in the luminol-added system.

  1. The NO + O and NO + O sub 3 reactions. 2. Analysis of NO sub 2 continuum chemiluminescence

    SciTech Connect

    Adler-Golden, S.M. (Spectral Sciences, Inc., Burlington, MA (USA))

    1989-01-26

    The NO{sub 2}* continuum chemiluminescence from the NO + O and NO + O{sub 3} reactions is simulated by using the model presented in the preceding paper. The model's validity is supported by the good agreement between calculated and experimental NO + O continuum spectra. A calculation of NO + O{sub 3} chemiluminescence assuming reaction on the ground potential energy surface alone is found to give reasonable agreement with experimental data on the continuum shape, absolute intensity, half-quenching pressure, and activation energy. This finding casts doubt on the popular hypothesis that NO{sub 2}* is formed in the NO + O{sub 3} reaction via an excited electronic channel.

  2. A Greener Chemiluminescence Demonstration

    ERIC Educational Resources Information Center

    Jilani, Osman; Donahue, Trisha M.; Mitchell, Miguel O.

    2011-01-01

    Because they are dramatic and intriguing, chemiluminescence demonstrations have been used for decades to stimulate interest in chemistry. One of the most intense chemiluminescent reactions is the oxidation of diaryl oxalate diesters with hydrogen peroxide in the presence of a fluorescer. In typical lecture demonstrations, the commercially…

  3. Collision energy dependence of the chemiluminescent reaction: Ba+N/sub 2/O. -->. BaO+N/sub 2/

    SciTech Connect

    Alcaraz, C.; de Pujo, P.; Cuvellier, J.; Mestdagh, J.M.

    1988-08-15

    The chemiluminescence spectrum of the reaction Ba+N/sub 2/O..-->..BaO+N/sub 2/ has been studied using a crossed beam apparatus as a function of the collision energy over the range 0.1--0.6 eV. The relative importance of its red wing increases as the collision energy is raised. Moreover, the cross section associated to chemiluminescence at a wavelength lambda of the range 450--700 nm goes through a maximum as the collision energy is varied, the position of which depends significantly on the value of lambda. The importance of this effect cast some doubt about works, where the energy dependence of the ''total'' chemiluminescence is measured using a detector which does not have a constant response over the wide range of the chemiluminescence. The analysis of the present results reveals that increasing the collision energy results in lowering the average vibrational excitation present in the emitting states A /sup 1/..sigma../sup +/ and A' /sup 1/Pi of the product BaO.

  4. The NO + O and NO + O sub 3 reactions. 1. Analysis of NO sub 2 vibrational chemiluminescence

    SciTech Connect

    Adler-Golden, S.M. (Spectral Sciences, Inc., Burlington, MA (USA))

    1989-01-26

    Low-resolution NO{sub 2}{nu}{sub 1} + {nu}{sub 3} and {nu}{sub 3} chemiluminescence spectra from the NO + O and NO + O{sub 3} reactions are simulated with a stepladder kinetic/spectroscopic model. The model uses simple parametrizations of radiative and collisional processes and assumes equipartition of electronic and vibrational energy. Good agreement is obtained with measurements of the {nu}{sub 1} + {nu}{sub 3} band shape and absolute intensity.

  5. The dynamics of surface-catalyzed reactions studied by infrared chemiluminescence of the CO and CO{sub 2} products

    SciTech Connect

    Watanabe, K.; Uetsuka, H.; Ohnuma, H.; Kunimori, K. [Univ. of Tsukuba, Ibaraki (Japan)

    1996-12-31

    The infrared chemiluminescence technique has been applied to the selective formation of syngas (CO + H{sub 2}) from the oxidation of small alkanes on Pt, the decomposition and oxidation of CH{sub 3}OH and HCOOH on Pt and Ni, and CO oxidation on Pd(111) and Pd(110). The different internal (vibrational and rotational) energy states of the CO and CO{sub 2} products have been observed, which reflect the difference in the dynamics of these reactions.

  6. Copper(II)-Alizarin Red S complex as an efficient chemiluminescent probe for the detection of human serum proteins after polyacrylamide gel electrophoresis.

    PubMed

    Wang, Zhenzhen; Liu, Xia; Baeyens, Willy R G; Delanghe, Joris R; Ouyang, Jin

    2008-12-01

    A novel chemiluminescent probe, copper(II)-Alizarin Red S (ARS) complex, for the detection of human serum proteins after polyacrylamide gel electrophoresis (PAGE) is described. The detection is based on the binding of the copper(II)-ARS complex to proteins and the catalytic activity of copper(II) in the luminol-hydrogen peroxide chemiluminescence (CL) system. Various proteins are directly detected in polyacrylamide gels, avoiding tedious transferring procedures. In the present study, the possible reaction mechanism and sensitivity evaluation are analyzed. The experimental conditions such as solution concentration, complex ratio, and washing reagents are likewise optimized. The proposed method offers simple, fast, and sensitive detection of serum proteins. As a novel chemiluminescent detection method, it shows significant analytical potential in biochemistry. PMID:19367697

  7. Chemiluminescence detection of hydrazine vapor.

    PubMed

    Collins, G E; Latturner, S; Rose-Pehrsson, S L

    1995-04-01

    An efficient, real-time chemiluminescence detector for hydrazine vapor, N(2)H(4)(g), is described, capable of monitoring sub part-per-billion levels of hydrazine in air. The catalytic oxidation of hydrazine by colloidal platinum forms an intermediate, oxidizing agent (e.g. OH or OOH) which subsequently oxidizes luminol, generating a chemiluminescence signal that is proportional to the hydrazine concentration. Major components of the instrument include a photomultiplier tube (PMT), a short length of glass tubing coiled directly in front of the PMT cathode surface, a vacuum pump for sampling the air, and a peristaltic pump for circulating the liquid reagent. The liquid reagent, a basic solution (pH 13) of luminol and colloidal platinum, is continuously recycled. The detection sequence is initiated by pumping the hydrazine vapor through a short length of teflon tubing that is concurrently transporting the liquid reagent. The liquid is separated from the gas stream in an impinger and quickly pumped to the PMT. We have evaluated the effect of solution pH, luminol and platinum concentrations, and air and liquid flow rates on the analytical characteristics of this system. A linear, dynamic detection range for hydrazine has been obtained from 1 to 2000 ppb in air, with an instrument response that is fully reversible and achieves plateau response in less than 2 min. PMID:18966262

  8. Multiple signal amplification electrogenerated chemiluminescence biosensors for sensitive protein kinase activity analysis and inhibition.

    PubMed

    Wang, Zonghua; Yan, Zhiyong; Sun, Na; Liu, Yang

    2015-06-15

    A novel electrogenerated chemiluminescence (ECL) biosensor was built for the detection of kinase activity based on multiple signal amplification nanoprobes. In this strategy, the Xanthine oxidase (XOD) and 5'-phosphate group end DNA conjugated AuNPs was integrated with the phosphorylated peptide by Zr(4+). The XOD on gold nanoparticles can catalyze dissolved oxygen to produce H2O2 in the presence of hypoxanthine (HA) which acts as a coreactor for luminol ECL reaction. In addition, due to the excellent catalytic activity of gold nanoparticle toward luminol ECL reaction and its large surface area that can accommodate large number of XOD and DNA on the surface, the ECL signal of luminol was significantly amplified, affording a highly sensitive ECL analysis of kinase activity. The as-proposed biosensor presents a low detection limit of 0.09UmL(-1) for protein kinase A (PKA) activity, wide linear range (from 0.1 to 10UmL(-1)) and excellent stability even in serum samples. This biosensor can also be applied for quantitative kinase inhibitor evaluation. The robust ECL biosensor provides a valuable tool for the high throughput assay in the applications of clinic diagnostic and therapeutic. PMID:25682506

  9. Rate Determination of the CO2* Chemiluminescence Reaction CO + O + M = CO2* + M

    E-print Network

    Kopp, Madeleine Marissa, 1987-

    2012-10-15

    The use of chemiluminescence measurements to monitor a range of combustion processes has been a popular area of study due to their reliable and cost-effective nature. Electronically excited carbon dioxide (CO2*) is known for its broadband emission...

  10. Highly sensitive homogenous chemiluminescence immunoassay using gold nanoparticles as label

    NASA Astrophysics Data System (ADS)

    Luo, Jing; Cui, Xiang; Liu, Wei; Li, Baoxin

    2014-10-01

    Homogeneous immunoassay is becoming more and more attractive for modern medical diagnosis because it is superior to heterogeneous immunoassay in sample and reagent consumption, analysis time, portability and disposability. Herein, a universal platform for homogeneous immunoassay, using human immunoglobulin G (IgG) as a model analyte, has been developed. This assay relies upon the catalytic activity of gold nanoparticles (AuNPs) on luminol-AgNO3 chemiluminescence (CL) reaction. The immunoreaction of antigen and antibody can induce the aggregation of antibody-functionalized AuNPs, and after aggregation the catalytic activity of AuNPs on luminol-AgNO3 CL reaction is greatly enhanced. Without any separation steps, a CL signal is generated upon addition of a trigger solution, and the CL intensity is directly correlated to the quantity of IgG. The detection limit of IgG was estimated to be as low as 3 pg/mL, and the sensitivity was better than that of the reported AuNPs-based CL immunoassay for IgG.

  11. Chemiluminescence flow sensor for folic acid with immobilized reagents.

    PubMed

    Song, Z; Zhou, X

    2001-11-01

    A novel chemiluminescence (CL) sensor for folic acid combined flow-injection (FI) technology was presented in this paper. The analytical reagents involved in the CL reaction, including luminol and hexacyanoferrate(III), were both immobilized on an anion-exchange column in FI system. The CL signal produced by the reaction between luminol and hexacyanoferrate(III), which were eluted from the column through sodium phosphate injection, was decreased in the presence of folic acid. The CL emission was correlated with the folic acid concentration in the range from 0.01 to 15 microg ml(-1), and the detection limit was 3.5 ng ml(-1) folic acid (3sigma). At a flow rate of 2.0 ml min(-1), including sampling and washing, could be performed in 2 min with a relative standard deviation of < 2.5%. The flow sensor could be reused more than 300 times and has been applied to the analysis of folic acid in pharmaceutical preparations. and the recovery was from 97.4% to 100.4%. PMID:11765783

  12. Determination of levodopa by capillary electrophoresis with chemiluminescence detection.

    PubMed

    Zhao, Shulin; Bai, Wenling; Wang, Bing; He, Min

    2007-08-15

    A rapid and simple method using capillary electrophoresis (CE) with chemiluminescence (CL) detection was developed for the determination of levodopa. This method was based on enhance effect of levodopa on the CL reaction between luminol and potassium hexacyanoferrate(III) (K(3)[Fe(CN)(6)]) in alkaline aqueous solution. CL detection employed a lab-built reaction flow cell and a photon counter. The optimized conditions for the CL detection were 1.0 x 10(-5)M luminol added to the CE running buffer and 5.0 x 10(-5)M K(3)[Fe(CN)(6)] in 0.6M NaOH solution introduced postcolumn. Under the optimal conditions, a linear range from 5.0 x 10(-8) to 2.5 x 10(-6)M (r=9991), and a detection limit of 2.0 x 10(-8)M (signal/noise=3) for levodopa were achieved. The precision (R.S.D.) on peak area (at 5.0 x 10(-7)M of levodopa, n=11) was 4.1%. The applicability of the method for the analysis of pharmaceutical and human plasma samples was examined. PMID:19071861

  13. Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory

    PubMed Central

    Khadem-Ansari, Mohammad-Hassan; Omrani, Mir-Davood; Rasmi, Yousef; Ghavam, Arsalan

    2014-01-01

    Background: Hepatitis B virus (HBV) is the most common significant chronic viral infection world-wide. Hepatitis B surface antigen (HBsAg) has been the principal target for laboratory testing to identify active infection by HBV. We aimed to find out diagnostic validity of the Liaison chemiluminescent method compared to the polymerase chain reaction (PCR) method for HBV detection in the routine clinical diagnostic laboratory. Materials and Methods: From 350 patients suspicious of having infection with HBV, serum samples were separated and used for testing HBsAg by two methods of Liaison chemiluminescent immunoassay, with HBsAg confirmatory test and PCR method. Results: According to the PCR results as assumed as gold standard method with 100% sensitivity and specificity, detection rate sensitivity of chemiluminescent with confirmatory test was 96% and its specificity was 100%, and for chemiluminescent without confirmatory test sensitivity and specificity were 100% and 70%, respectively. Also for chemiluminescent with confirmatory test, positive predictive value (PPV) was 100% and its negative predictive value (NPV) was 97%, compared to chemiluminescent without confirmatory test with PPV and NPV equal to 71% and 100%, respectively. Conclusions: It is possible to conclude that in the majority of the HBV cases, the diagnostic value of chemiluminescent method compared to the PCR method is acceptable, except in low indexes positive cases that need further investigation with the PCR method. PMID:24949287

  14. On-line chemiluminescence determination of mitoxantrone by capillary electrophoresis.

    PubMed

    Han, Suqin; Wang, Huili

    2010-10-15

    A novel capillary electrophoresis (CE) with chemiluminescence (CL) detection method for the determination of mitoxantrone (MTX) has been developed, which based on the CL reaction of potassium ferricyanide with luminol in sodium hydroxide medium sensitized by MTX. Under optimum analytical conditions, MTX is determined over the range of 7.0×10(-8)-1.0×10(-6)M with a detection limit of 1.0×10(-8)M. The relative standard deviation (RSD) was 3.7%, 2.6% and 3.0% for 7.0×10(-8), 5.0×10(-7) and 1.0×10(-6)M MTX (n=11), respectively. In laboratory-built CE-CL apparatus, the proposed method has been applied to determination of MTX in commercial drug and spiked in human urine and plasma with satisfactory results. PMID:20817570

  15. Plant tissue-based chemiluminescence biosensor for ethanol.

    PubMed

    Huang, Yuming; Wu, Fangqiong

    2006-07-01

    A plant tissue-based chemiluminescence biosensor for ethanol based on using mushroom (Agaricus bisporus) tissue as the recognition element is proposed in this paper. The principle for ethanol sensing relies on the luminol-potassium hexacyanoferrate(III)-hydrogen peroxide transducer reaction, in which hydrogen peroxide is produced from the ethanol enzymatic catalytic oxidation by oxygen under the catalysis of alcohol oxidase in the tissue column. Under optimum conditions, the method allowed the measurement of ethanol in the range of 0.001 - 2 mmol/l with a detection limit (3 sigma) of 0.2 micromol/l. The relative standard deviation (RSD) was 4.14% (n = 11) for 0.05 mmol/l ethanol. The proposed method has been applied to the determination of ethanol in biological fluids and beverages with satisfactory results. PMID:16837747

  16. A portable and low cost equipment for flow injection chemiluminescence measurements.

    PubMed

    Rocha, Fábio R P; Ródenas-Torralba, Eva; Reis, Boaventura F; Morales-Rubio, Angel; Guardia, Miguel de la

    2005-10-15

    A compact, reliable and low cost flow injection chemiluminescence system is described. The flow system consists of a set of solenoid micro-pumps that can dispense reproductive micro-volumes of solutions. The luminometer was based on a coiled cell constructed from polyethylene tubing that was sandwiched between two large area photodiodes. The whole equipment costs about US$ 750 and weights ca. 3 kg. Equipment performance was evaluated by measuring low concentrations of hydrogen peroxide by oxidation of luminol and for the determination of ammonium, based on its inhibition of the luminescence provided by the reaction of luminol and sodium hypochlorite. Linear responses were achieved within 1.0-80 micromol L(-1) H(2)O(2) and 0.6-60 micromol L(-1) NH(4)(+) with detection limits estimated as 400 nmol L(-1) H(2)O(2) and 60 nmol L(-1) NH(4)(+) at the 99.7% confidence level. Coefficients of variation were 1.0 and 1.8%, estimated for 20 micromol L(-1) H(2)O(2) and 15 micromol L(-1) NH(4)(+) (n=20), respectively. Reagent consumption of 55 microg luminol, effluent volume of 950 microL per determination and sampling rate of 120 samples per hour were also achieved. PMID:18970223

  17. Glucocorticosteroids and in vitro effects on chemiluminescence of isolated bovine blood granulocytes

    Microsoft Academic Search

    Dagmar Hoeben; Christian Burvenich; Anne-Marie Massart-Leën

    1998-01-01

    The effects of glucocorticosteroids on respiratory burst of bovine granulocytes were studied in vitro by means of (1) chemiluminescence (luminol-dependent, phorbol 12-myristate 13-acetate (PMA)-stimulated), (2) a cell-free chemiluminescence assay, and (3) a myeloperoxidase assay. Significant effects on cellular chemiluminescence were only observed at the highest, not obtainable in vivo, concentration for all drugs except betamethasone. Prednisolone induced inhibition at therapeutic

  18. Chemiluminescent and Bioluminescent Techniques

    Microsoft Academic Search

    Larry J. Kricka

    intermediates that decay to a ground state with the emission of light. BL is a special type of CL found in nature, in which the light emission is facilitated by an enzyme(luciferase) or a photoprotein. The overall quan- tum yield of a CL reaction (the number of photons emitted\\/number of molecules reacting) is generally in the range 1-10% (e.g., luminol

  19. Micro determination of cortisol and cortisone in umbilical cord blood by chemiluminescent high-performance liquid chromatography.

    PubMed

    Hasegawa, Takeshi; Kubo, Hiroaki; Shinozaki, Koichi; Nowatari, Masahiko; Ishii, Masahiro

    2010-06-01

    A simple, sensitive and specific chemiluminescent high-performance liquid chromatography method, based on the luminol reaction, for determination of serum cortisol and cortisone, was established. In infants, placental 11beta-hydroxysteroid dehydrogenase type 2 enzyme (11beta-HSD2) activity may affect adrenal function early after birth. The cortisol-cortisone ratio of serum concentrations in umbilical cord blood is an indicator of placental 11beta-HSD2 activity. The optimum conditions for the luminol reaction were determined to be 1.5 mM luminol, 0.6 M sodium hydroxide, 0.15 mm potassium hexacyanoferrate(III) and 200 mM potassium hexacyanoferrate (II). The calibration curves for cortisol and cortisone exhibited good linearity. The correlation coefficients of the calibration curves were 0.996. The intra- and inter-day precisions were in the ranges: cortisol 7.0-12.2 and 4.4-9.2%, cortisone 5.3-7.0 and 6.2-9.9%. The recoveries of these steroids were in the ranges: cortisol 97-105%, cortisone 94-102%. The limits of detection were as follows: cortisol, 0.17 microg/dl; cortisone 0.15 microg/dl. This assay could be successfully applied to determination of the cortisol-cortiosone ratio of serum concentrations in umbilical cord bloods. PMID:19816851

  20. Glow-Discharge Shock Tube for Studying Chemiluminescent, Surface-Catalytic, and Gas-Phase Reaction Rates; Temperature Dependence of NO&sngbnd;O and CO&sngbnd;O Chemiluminescence

    Microsoft Academic Search

    R. A. Hartunian; W. P. Thompson; E. W. Hewitt

    1966-01-01

    The ordinary glow-discharge tube has been used extensively to study both surface and gas-phase recombination rates, as well as chemiluminescent reactions, typically at room temperature. The combination of a glow-discharge flow tube with a shock-tube driver to provide a considerable extension of atom fluxes, temperatures, and densities for the study of these kinetic processes is described. In operation, the ordinary

  1. Simultaneous detection of monoethanolamine, diethanolamine, and triethanolamine by HPLC with a chemiluminescence reaction and online derivatization to tertiary amine.

    PubMed

    Niina, Nobumitsu; Kodamatani, Hitoshi; Uozumi, Kayoko; Kokufu, Yuki; Saito, Keiitsu; Yamazaki, Shigeo

    2005-05-01

    In this paper we propose a new postcolumn detection method for compounds having primary, secondary, and tertiary amine moieties. The primary and secondary amine are delivatized by a reaction with epichlorohydrin having an epoxy moiety in a reaction coil to yield a tertiary amine with subsequent chemiluminescence detection using [Ru(bpy)3]3+. The liner values of the calibration curves of monoethanolamine (MEA), diethanolamine (DEA) and triethanolamine (TEA) were 0.02 - 1.0 nmol (r2 = 0.9986), 0.02 - 0.5 nmol (r2 = 0.9993) and 0.1 - 1.0 nmol (r2 = 0.9482), respectively. Also, the detection limits (S/N = 3) of MEA, DEA and TEA were 30, 25 and 40 pmol, respectively. The amount of DEA and TEA in shaving cream (60 microg/20 microL) were found to be 0.3 nmol and 14 nmol, respectively, by the proposed method. PMID:15913135

  2. Chemiluminescence in the reactions Mn + O sub 2 , NO sub 2 , CO sub 2 , and SO sub 2

    SciTech Connect

    Levy, M.R. (Newcastle Polytechnic, Newcastle upon Tyne (United Kingdom))

    1991-10-31

    A laser-ablated pulsed beam of Mn atoms has been employed to determine the translational energy dependence of the chemiluminescent processes Mn(a{sup 6}S,a{sup 6}D{sub J},{hor ellipsis}) + RO {yields} MnO{sup *}(A{sup 6}{Sigma}{sup +}) + R, where R = O, NO, CO, and SO. Analysis of the excitation functions by microcanonical transition-state theory indicates that only a very few Mn states contribute significantly - most probably a{sup 6}D{sub J}, a{sup 4}D{sub J}, and perhaps (in the O{sub 2} case only) z{sup 8}P{sub J}. With the exception of Mn{sup *}(a{sup 4}D{sub J}) + O{sub 2} and CO{sub 2}, the results are consistent with simple adiabatic state correlations. All a{sup 6}D{sub J}-assigned reactions appear to proceed through a potential well, with a barrier in the exit channel; but, as collision energy increases, most, if not all, of the excitation functions change to the simple line-of-centers form, indicating that the well no longer influences the transition-state dynamics. Depletion in the chemiluminescence at higher energies still is attributed to recrossing.

  3. CMOS arrays as chemiluminescence detectors on microfluidic devices.

    PubMed

    Rodrigues, Eunice R G O; Lapa, Rui A S

    2010-05-01

    A simple, low-cost process to integrate complementary metal oxide semiconductor array detectors (CMOSAD) for chemiluminescence is presented, evaluated, and applied to the determination of nitrite in ground water samples. CMOS arrays of different brands (obtained from commercial image sensors) were adapted as chemiluminescence detectors on microfluidic devices. The performance of the CMOSADs was evaluated in the visible zone of the spectrum using a tungsten halogen lamp as light source. Intrinsic parameters assessed included signal stability, spectral response, dark current, and signal-to-noise ratio. Thereafter, the CMOSADs were integrated on microfluidic devices and their performances in quantitative analysis were assessed with the chemiluminometric reaction of hydrogen peroxide with luminol, catalyzed with hexacyanoferrate (III). The parameters assessed were sensitivity, linear range, detection limit, reproducibility, correlation coefficient of the calibration curves, and baseline drift during measurements. The CMOSAD with the best performance was selected to assess the applicability of the developed microfluidic devices with the integrated detector. The microfluidic system permitted the determination of nitrite with both good precision and good recovery values in the analysis of ground water samples. Integration was easily achieved and enabled the development of a simple, low-cost, and feasible alternative to conventional detectors. PMID:20177663

  4. Photoinduced chemiluminescence of pharmaceuticals.

    PubMed

    Gómez-Taylor, B; Palomeque, M; García Mateo, J V; Martínez Calatayud, J

    2006-05-01

    A screening test for the forward development of chemiluminescence systems able to determine pharmaceutical compounds is reported. The test is based on the on-line photodegradation of the drugs by using a photoreactor consisting of 697 cmx0.5 mm PTFE tubing helically coiled around an 8 W low-pressure mercury lamp. Photodegraded pharmaceuticals are detected by direct chemiluminescence of the resulting photofragments and their subsequent reaction with potassium permanganate in sulphuric acid medium as oxidant. The screening comprised 97 compounds with different molecular structures and relevant members of the most important families of pharmaceuticals are tested (amino acids, carboxylic acids, nitrocompounds, phenyl-alkyl and aromatic amines, sulphonic acid amides, polycarbocyclics, monocyclic N-containing heterocyclics, bicyclic N-containing heterocyclics, tricyclic N-containing heterocyclics, N-S containing heterocyclics...). Due to the relevant influence of the medium for the photodegradation a wide range of pH's and buffer solutions were studied. The proposed strategy (photoinduced chemiluminescence, Ph-CL) allows the development of systems for the determination of many pharmaceuticals which do not present "native" chemiluminescence (e.g. chloramphenicol, dextromethorpham, riboflavin, ephedrine, piperazinamide, chlotrimazole, theophylline...). Moreover, Ph-CL allows to increase the sensitivity of chemiluminescence procedures based on direct chemiluminescence detection (e.g. sulphonamides, thiazides, nicontinamide, nortryptiline, levamisole, phenylbarbituric acid...). PMID:16413738

  5. Phenylboronic acid immunoaffinity reactor coupled with flow injection chemiluminescence for determination of alpha-fetoprotein.

    PubMed

    Wu, Yafeng; Zhuang, Yafeng; Liu, Songqin; He, Lin

    2008-12-23

    A reusable and sensitive immunoassay based on phenylboronic acid immunoaffinity reactor in combination with flow injection chemiluminescence (CL) for determination of glycoprotein was described. The reactor was fabricated by immobilizing 3-aminophenylboronic acid (APBA) on glass microbeads with gamma-glycidoxypropyltrimethoxysilane (GPMS) as linkage. The alpha-fetoprotein (AFP) could be easily immobilized on the APBA coated beads through sugar-boronic interaction. After an off-line incubation, the mixture of the analyte AFP with horseradish peroxidase-labeled AFP antibody (HRP-anti-AFP) was injected into the reactor. This led the trapping of free HRP-anti-AFP by the surface coated AFP on glass beads. The trapped HRP-anti-AFP was detected by chemiluminescence due to its sensitizing effect on the reaction of luminol and hydrogen peroxide. Under optimal conditions, the chemiluminescent signal was proportional to AFP concentration in the range of 10-10 0 ng m L(-1). The whole assay process including regeneration of the reactor could be completed within 31 min. The proposed system showed acceptable detection and fabrication reproducibility, and the results obtained with the present method were in acceptable agreement with those from parallel single-analyte test of practical clinical sera. The described method enabled a low-cost, time saving and was potential to detect the serum AFP level in clinical diagnosis. PMID:19012831

  6. NO2 measurement by chemiluminescence

    NASA Technical Reports Server (NTRS)

    Conway, E. J.; Rogowski, R. S.; Richards, R. R.

    1979-01-01

    Compact device monitors specific chemiluminescent reaction of heated solid material such as 3,5 diaminobezoic or polyvinyl alcohol after contact with gas sample to detect and quantify nitrogen dioxide concentration.

  7. The Chemiluminescence Homepage

    NSDL National Science Digital Library

    Dr. Thomas Chasteen at Sam Houston State University created this compilation of sites and texts related to chemiluminescence, a process where "energy in the form of light is released from matter because of a chemical reaction." The site contains Quick Time animations, descriptions of related phenomena, links to related sites, and two bibliographies, one targeting advanced readers and the other targeting K-12 readers.

  8. A novel chemiluminescence assay of mitoxantrone based on diperiodatocuprate(III) oxidation.

    PubMed

    Yao, Hanchun; Zhang, Min; Zeng, Wenyuan; Zeng, Xiaoying; Zhang, Zhenzhong

    2014-01-01

    A novel and strong chemiluminescence (CL) of luminol with diperiodatocuprate (K5[Cu(HIO6)2]) was observed in alkaline medium. After the addition of mitoxantrone (MTX) into this system, the CL intensity could be greatly inhibited by MTX. Based on the phenomenon, a sensitive CL method was established for analysis of MTX combining with flow injection technology. Under optimum experimental conditions, the CL intensity was linearly related to the logarithm concentration of MTX from 5.0×10(-9)-1.0×10(-7) g/ml with the detection limit of 1.1×10(-9) g/ml (S/N=3). The relative standard deviation was 1.2% for 5.0×10(-8) g/ml of MTX. The proposed method was successfully applied for determination of MTX in pharmaceutical preparations and biological fluids. The possible CL reaction mechanism was also discussed briefly. PMID:24121650

  9. A novel chemiluminescence assay of mitoxantrone based on diperiodatocuprate(III) oxidation

    NASA Astrophysics Data System (ADS)

    Yao, Hanchun; Zhang, Min; Zeng, Wenyuan; Zeng, Xiaoying; Zhang, Zhenzhong

    2014-01-01

    A novel and strong chemiluminescence (CL) of luminol with diperiodatocuprate (K5[Cu(HIO6)2]) was observed in alkaline medium. After the addition of mitoxantrone (MTX) into this system, the CL intensity could be greatly inhibited by MTX. Based on the phenomenon, a sensitive CL method was established for analysis of MTX combining with flow injection technology. Under optimum experimental conditions, the CL intensity was linearly related to the logarithm concentration of MTX from 5.0 × 10-9-1.0 × 10-7 g/ml with the detection limit of 1.1 × 10-9 g/ml (S/N = 3). The relative standard deviation was 1.2% for 5.0 × 10-8 g/ml of MTX. The proposed method was successfully applied for determination of MTX in pharmaceutical preparations and biological fluids. The possible CL reaction mechanism was also discussed briefly.

  10. Decrease of neutrophils chemiluminescence during exposure to low-power laser infrared radiation

    NASA Astrophysics Data System (ADS)

    Czuba, Zenon P.; Adamek, Mariusz; Krol, Wojciech; Sieron, Aleksander; Cieslar, Grzegorz

    1995-01-01

    The neutrophil is the cell in which phagocyting and transforming of some exogeneous agents results in marked stimulation of nonmitochondrial respiratory chain activity (respiratory burst). In our experiment we focused on determining the level of chemiluminescence (CL) of stimulated neurotrophils during and after irradiation, measuring the photon emission intensity in 6 second's intervals. We used Ga-Al-As pulsed laser (wavelength 904 nm, mean power 8,9 mW, Alpha-Electronics GmbH, Germany) which was placed over the tube containing the suspension of guinea pig peritoneal neurotrophils (2X106 cells/ml). The sensitivity range of used photomultiplier (9514s, THORN EMI, Middlesex, England) was 300-600 nm, which allowed us to measure the CL of neutrophils while being irradiated. The neutrophils were stimulated by phorbol myristate acetate (PMA) and CL intensified by luminol. The decay of luminol-dependent CL of neutrophils may be described by hyperbolic function curve. We switched the laser radiation on for 20 s, 60 s and 300 s and each time we observed the same reaction: the about 20% decrease of intensity of CL immediately after beginning the irradiation. The CL remained on decreased level during the whole period of irradiation reaching immediately the level of CL intensity characteristic for decay curve (20% increase), just after switching off the laser. Only after the longest irradiation time (300 s) we observed CL being higher and inconsistent with decay curve for several minutes. The type of reaction was always the same, regardless to the point of CL decay curve at which laser radiation was applied. The same changes of Cl we obtained irradiating the enzymatic system: horseradish peroxidase (HRP)-luminol - H2O2.

  11. 3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein.

    PubMed

    Sakharov, Ivan Yu; Demiyanova, Alexandra S; Gribas, Anastasia V; Uskova, Natalia A; Efremov, Evgeny E; Vdovenko, Marina M

    2013-10-15

    Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10'-phenothiazinyl)propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09 pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5 ng mL(-1). The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits. PMID:24054611

  12. Sensitive determination of carbidopa through the electrochemiluminescence of luminol at graphene-modified electrodes.

    PubMed

    Hosseini, Morteza; Mirzanasiri, Nooshin; Rezapour, Morteza; Sheikhha, Mohammad Hasan; Faridbod, Farnoush; Norouzi, Parviz; Ganjali, Mohammad Reza

    2015-06-01

    Using the concept of electrogenerated chemiluminescence (ECL), a sensitive analytical method for the determination of carbidopa is described. Electro-oxidation of carbidopa on the surface of a graphene oxide (GO)-modified gold electrode (GE) leads to enhancement of the weak emission of oxidized luminol. Under optimum experimental conditions, the ECL signal increases linearly with increasing carbidopa concentrations over a range of 1.0?×?10(-9) -1.7?×?10(-7) ?M, with a detection limit of 7.4?×?10(-10) ?M. The proposed ECL method was successfully used for the determination of carbidopa in urine samples. Copyright © 2014 John Wiley & Sons, Ltd. PMID:25131492

  13. Change in the chemiluminescence reactivity pattern during in vitro differentiation of human monocytes to macrophages

    Microsoft Academic Search

    Thomas W. Jungi; Ernst Peterhans

    1988-01-01

    We determined the luminol-enhanced chemiluminescence (CL) of fresh human monocytes and monocytes cultured for 1–14 days in vitro, within hydrophobic membranes, using a variety of stimuli known to trigger the respiratory burst of phagocytes. It was assured that CL emerged from an adherent subpopulation of mononuclear cells; polymorphonuclear leukocytes (PMN) contaminating mononuclear leukocytes (MNL) contributed little, if anything, to the

  14. Chemiluminescence reaction kinetics-resolved multianalyte immunoassay strategy using a bispecific monoclonal antibody as the unique recognition reagent.

    PubMed

    Ouyang, Hui; Wang, Limin; Yang, Shijia; Wang, Wenwen; Wang, Lin; Liu, Fengquan; Fu, Zhifeng

    2015-03-01

    The multianalyte immunoassay (MIA) has attracted increasing attention due to its high sample throughput, short assay time, low sample consumption, and reduced overall cost. However, up to now, the reported MIA methods commonly require multiple antibodies since each antibody can recognize only one antigen. Herein, a novel bispecific monoclonal antibody (BsMcAb) that could bind methyl parathion and imidacloprid simultaneously was produced by a hybrid hybridomas strategy. A chemiluminescence (CL) reaction kinetics-resolved strategy was designed for MIA of methyl parathion and imidacloprid using the BsMcAb as the unique recognition reagent. Horseradish peroxidase (HRP) and alkaline phosphatase (ALP) were adopted as the signal probes to tag the haptens of the two pesticides due to their very different CL kinetic characteristics. After competitive immunoreactions, the HRP-tagged methyl parathion hapten and the ALP-tagged imidacloprid hapten were simultaneously bound to the BsMcAb since there were two different antigen-binding sites in it. Then, two CL reactions were simultaneously triggered by adding the CL coreactants, and the signals for methyl parathion and imidacloprid detections were collected at 0.6 and 1000 s, respectively. The linear ranges for methyl parathion and imidacloprid were both 1.0-500 ng/mL, with detection limits of 0.33 ng/mL (S/N = 3). The proposed method was successfully used to detect pesticides spiked in ginseng and American ginseng with acceptable recoveries of 80-118%. This proof-of-principle work demonstrated the feasibility of MIA using only one antibody. PMID:25622025

  15. Chemiluminescent reaction of Ba(3P) with N2O at hyperthermal collision energies: Rotational alignment of the BaO(A 1?+) product

    NASA Astrophysics Data System (ADS)

    Rossa, Maximiliano; Rinaldi, Carlos A.; Ferrero, Juan C.

    2007-08-01

    The chemiluminescent reaction Ba(6s6p 3P)+N2O was studied at an average collision energy of 1.56 eV in a beam-gas arrangement. Ba(3P) was produced by laser ablation of barium, which resulted in a broad collision energy distribution extending up to ~5.7 eV. A series of experiments was made to extract the Ba(3P) contribution to chemiluminescence from that corresponding to Ba 6s2 1S0 and 6s5d 3D, which are the other two most populated states in the atomic beam. The fully dispersed polarized chemiluminescence spectra at 400-600 nm from the title reaction were recorded and assigned to a BaO molecule excited in the A 1?+ level. In addition, the average and wavelength-resolved degrees of polarization associated to the parallel BaO(A 1?+-->X 1?+) emission are reported. The analysis of the average polarization degree show that the BaO(A 1?+) product is significantly aligned, suggesting that the reaction mechanism is predominantly direct. The product rotational alignment was found to depend markedly on the emission wavelength, which revealed a negative correlation with the BaO(A 1?+) product vibrational state. On the basis of experimental and theoretical investigations on the reactions of N2O with both the 1S0, 3D, and 1P1 states of Ba and the lighter group 2 atoms, it is suggested that the Ba(3P) reaction involves a charge transfer at relatively short reagent separations and that restricted collision geometries at the highest velocity components of the broad distribution are necessary to rationalize the data.

  16. Chemiluminescence development after initiation of Maillard reaction in aqueous solutions of glycine and glucose: nonlinearity of the process and cooperative properties of the reaction system

    NASA Astrophysics Data System (ADS)

    Voeikov, Vladimir L.; Naletov, Vladimir I.

    1998-06-01

    Nonenzymatic glycation of free or peptide bound amino acids (Maillard reaction, MR) plays an important role in aging, diabetic complications and atherosclerosis. MR taking place at high temperatures is accompanied by chemiluminescence (CL). Here kinetics of CL development in MR proceeding in model systems at room temperature has been analyzed for the first time. Brief heating of glycine and D-glucose solutions to t greater than 93 degrees Celsius results in their browning and appearance of fluorescencent properties. Developed In solutions rapidly cooled down to 20 degrees Celsius a wave of CL. It reached maximum intensity around 40 min after the reaction mixture heating and cooling it down. CL intensity elevation was accompanied by certain decoloration of the solution. Appearance of light absorbing substances and development of CL depended critically upon the temperature of preincubation (greater than or equal to 93 degrees Celsius), initial pH (greater than or equal to 11,2), sample volume (greater than or equal to 0.5 ml) and reagents concentrations. Dependence of total counts accumulation on a system volume over the critical volume was non-monotonous. After reaching maximum values CL began to decline, though only small part of glucose and glycin had been consumed. Brief heating of such solutions to the critical temperature resulted in emergence of a new CL wave. This procedure could be repeated in one and the same reaction system for several times. Whole CL kinetic curve best fitted to lognormal distribution. Macrokinetic properties of the process are characteristic of chain reactions with delayed branching. Results imply also, that self-organization occurs in this system, and that the course of the process strongly depends upon boundary conditions and periodic interference in its course.

  17. Micro-plate chemiluminescence enzyme immunoassay for aflatoxin B1 in agricultural products.

    PubMed

    Fang, Luqiu; Chen, Hui; Ying, Xitang; Lin, Jin-Ming

    2011-03-15

    In this work, a micro-plate chemiluminescence enzyme immunoassay by antibody-coated for the determination of aflatoxin B1 (AFB1) in agricultural products has been established. Aflatoxin B1 antibody (AFB1-Ab) was adsorbed physically on polystyrene micro-plate hole as solid phase antibody, which took place immunity-reaction between antigen and antibody with AFB1 standard solution or samples by direct competition. Luminol-hydrogen peroxide chemiluminescence system catalyzed by horseradish peroxidase (HRP) with p-iodophenol enhancement was used as signal detecting system. The effects of several factors, including composition and pH of coating solution, dilution ratio and amount of antibody and enzyme labeled antigen, time of antibody-coating, incubation and chemiluminescence reaction, and other relevant variables upon the immunoassay were studied and optimized. The linear range of proposed method for AFB1 was 0.05-10.0 ng g(-1) with a correlative coefficient of -0.9997. The sensitivity of the proposed method was 0.01 ng g(-1). The RSDs of intra- and inter-assay were less than 12.2% and 10.0%, respectively. This method has been successfully applied to the evaluation of AFB1 in agricultural products with recoveries of 79.8%, 101.9% and 115.4% for low, middle and high concentration samples, respectively. It shows a good correlation with the commercial available ELISA kit for AFB1 with correlative coefficient of 0.9098 indicating that the established CLEIA method can be used to determine AFB1 in real samples. PMID:21315923

  18. Study of catalytic reaction processes on the {gamma}-Al{sub 2}O{sub 3} chemiluminescence-based gas sensors

    SciTech Connect

    Utsunomiya, K.; Nakagawa, M.; Nishiyama, K.; Takechi, S. [Okayama Univ. of Science (Japan)] [and others

    1996-12-31

    The authors have investigated a new chemiluminescence (CL)-based gas sensor made of aluminum oxide ({gamma}-Al{sub 2}O{sub 3}) which emits CL during the catalytic oxidation of combustible vapors in air. The CL intensity is proportional to the concentration in the wide region from 1 to 1000 ppm of ethanol, butanol and acetone in air. However, it has a tendency to saturate in concentrations above 1000 ppm. For the detection of vapors in the environmental atmosphere, improvements of the sensitivity and the linear characteristics of the sensor are necessary. Catalytic reaction processes on the sensor were studied for this purpose.

  19. Fiber-Optic Chemiluminescent Biosensors for Monitoring Aqueous Alcohols and Other Water Quality Parameters

    NASA Technical Reports Server (NTRS)

    Verostko, Charles E. (Inventor); Atwater, James E. (Inventor); Akse, James R. (Inventor); DeHart, Jeffrey L. (Inventor); Wheeler, Richard R. (Inventor)

    1998-01-01

    A "reagentless" chemiluminescent biosensor and method for the determination of hydrogen peroxide, ethanol and D-glucose in water is disclosed. An aqueous stream is basified by passing it through a solid phase base bed. Luminol is then dissolved in the basified effluent at a controlled rate. Oxidation of the luminol is catalyzed by the target chemical to produce emitted light. The intensity of the emitted light is detected as a measure of the target chemical concentration in the aqueous stream. The emitted light can be transmitted by a fiber optic bundle to a remote location from the aqueous stream for a remote reading of the target chemical concentration.

  20. Intricacies of Redoxome Function Demonstrated with a Simple In vitro Chemiluminescence Method, with Special Reference to Vitamin B12 as Antioxidant

    PubMed Central

    Bøyum, A; Forstrøm, R J; Sefland, I; Sand, K L; Benestad, H B

    2014-01-01

    The homeostatic control of the redox system (the redoxome) in mammalian cells depends upon a large number of interacting molecules, which tend to buffer the electronegativity of cells against oxidants or reductants. Some of these components kill – at high concentration – microbes and by-stander normal cells, elaborated by professional phagocytes. We examined whether a simple, in vitro chemiluminescence set-up, utilizing redox components from human polymorphonuclear neutrophils (PMN) and red blood cells (RBC), could clarify some unexplained workings of the redoxome. PMN or purified myeloperoxidase (MPO) triggers formation of reactive oxygen species (ROS), quantified by light emission from oxidized luminol. Both PMN and RBC can generate abundant amounts of ROS, necessitating the presence of a high-capacity redoxome to keep the cellular electronegativity within physiological limits. We obtained proof-of-principle evidence that our assay could assess redox effects, but also demonstrated the intricacies of redox reactions. Simple dose–responses were found, as for the PMN proteins S100A9 (A9) and S100A8 (A8), and the system also revealed the reducing capacity of vitamin B12 (Cbl) and lutein. However, increased concentrations of oxidants in the assay mixture could decrease the chemiluminescence. Even more remarkable, A9 and NaOCl together stimulated the MPO response, but alone they inhibited MPO chemiluminescence. Biphasic responses were also recorded for some dose–response set-ups and are tentatively explained by a ‘balance hypothesis’ for the redoxome. PMID:25345916

  1. A Facile and Effective Chemiluminescence Demonstration Experiment

    ERIC Educational Resources Information Center

    Mohan, Arthur G.; Turro, Nicholas J.

    1974-01-01

    Describes a chemiluminescence system which can be used to demonstrate the effects of certain factors which affect the rate of reaction (temperature, concentration, catalysis, solvent, etc.), and to perform experiments relevant to the mechanism of the system. (SLH)

  2. Steady-state chemiluminescence of Eu-doped yttrium oxide crystal phosphors in the catalytic reaction of hydrogen oxidation

    NASA Astrophysics Data System (ADS)

    Nedrigaylov, Ievgen I.; Karpov, Eduard G.; Styrov, Vladislav V.

    2008-11-01

    We report on the luminescence of Eu 3+-doped yttrium oxide phosphors brought in contact with oxyhydrogen gas mixtures at temperatures much below the gas-phase ignition point. Chemical energy released in the catalytic hydrogen oxidation is passed directly to the electron subsystem of the powder and subsequently converted into the visible luminescence radiation at 2 × 10 -6 quantum yield per H 2O molecule produced. Luminescence kinetics at various conditions are investigated to reveal the constituent stages of the catalytic energy conversion process. This observation could have far-reaching consequences including possibilities for the development of original chemiluminescent light sources, and novel electrochemical energy converters.

  3. Controllable copper deficiency in Cu2-xSe nanocrystals with tunable localized surface plasmon resonance and enhanced chemiluminescence

    NASA Astrophysics Data System (ADS)

    Lie, Shao Qing; Wang, Dong Mei; Gao, Ming Xuan; Huang, Cheng Zhi

    2014-08-01

    Copper chalcogenide nanocrystals (CuCNCs) as a type of semiconductor that can also act as efficient catalysts are rarely reported. Herein, we study water-soluble size-controlled Cu2-xSe nanocrystals (NCs), which are copper deficient and could be prepared by a redox reaction with the assistance of surfactants. We found them to have strong near-infrared localized surface plasmon resonance (LSPR) properties originating from the holes in the valence band, and also catalytic activity of more than a 500-fold enhancement of chemiluminescence (CL) in a luminol-H2O2 system. Investigations into the mechanisms behind these results showed that the high concentration of free carriers in Cu2-xSe NCs, which are derived from their high copper deficiencies that make Cu2-xSe NCs both good electron donors and acceptors with high ionic mobility, could greatly enhance the catalytic ability of Cu2-xSe NCs to facilitate electron-transfer processes and the decomposition of H2O2 into OH&z.rad; and O2&z.rad;-, which are the commonly accepted key intermediates in luminol CL enhancement. Thus, it can be concluded that controllable copper deficiencies that are correlated with their near-infrared LSPR are critically responsible for the effective catalysis of Cu2-xSe NCs in the enhanced CL.Copper chalcogenide nanocrystals (CuCNCs) as a type of semiconductor that can also act as efficient catalysts are rarely reported. Herein, we study water-soluble size-controlled Cu2-xSe nanocrystals (NCs), which are copper deficient and could be prepared by a redox reaction with the assistance of surfactants. We found them to have strong near-infrared localized surface plasmon resonance (LSPR) properties originating from the holes in the valence band, and also catalytic activity of more than a 500-fold enhancement of chemiluminescence (CL) in a luminol-H2O2 system. Investigations into the mechanisms behind these results showed that the high concentration of free carriers in Cu2-xSe NCs, which are derived from their high copper deficiencies that make Cu2-xSe NCs both good electron donors and acceptors with high ionic mobility, could greatly enhance the catalytic ability of Cu2-xSe NCs to facilitate electron-transfer processes and the decomposition of H2O2 into OH&z.rad; and O2&z.rad;-, which are the commonly accepted key intermediates in luminol CL enhancement. Thus, it can be concluded that controllable copper deficiencies that are correlated with their near-infrared LSPR are critically responsible for the effective catalysis of Cu2-xSe NCs in the enhanced CL. Electronic supplementary information (ESI) available: Experimental section and additional figures for XRD, XPS, UV absorption, chemiluminescent spectra, SEM and TEM images. See DOI: 10.1039/c4nr02294g

  4. A novel chemiluminescence from the reaction of singlet oxygen with ?-diketonates of europium(III), neodymium(III) and ytterbium(III).

    PubMed

    Kazakov, Dmitri V; Safarov, Farit E

    2014-12-01

    Decomposition of 1,4-dimethylnaphthalene endoperoxide, which is the source of singlet oxygen, in the presence of ?-diketonates of europium(III), neodymium(III) and ytterbium(III) is accompanied by bright chemiluminescence (CL) in visible and near infra-red spectral region due to characteristic emission from the lanthanides at ?max = 615 and 710 nm ((5)D0?(7)F2 and (5)D0?(7)F4 transitions of Eu(3+)), 900 nm ((4)F3/2?(4)I9/2 transition of Nd(3+)) and 1000 nm ((2)F5/2?(2)F7/2 transition of Yb(3+)). Singlet oxygen is the key intermediate responsible for the observed CL, which is presumably generated by the reaction of (1)O2 with ligands of the complexes. The CL phenomenon discovered herein paves the way towards the development of lanthanide-based CL probe for (1)O2. PMID:25358493

  5. Binding study of lysozyme with Al(III) using chemiluminescence analysis.

    PubMed

    Liu, Jiangman; Luo, Kai; Song, Zhenghua

    2014-09-01

    The binding behavior of lysozyme with Al(III) is described using luminol as a luminescence probe by flow injection-chemiluminescence (FI-CL) analysis. It was found that the CL intensity of the luminol-lysozyme reaction could be markedly enhanced by Al(III), and the increase in CL intensity was linear with the Al(III) concentration over the range 0.3-30.0? pg ?mL(-1) , with a detection limit of 0.1?pg ?mL(-1) (3?). Based on the interaction model of lysozyme with Al(III), lg[(I?-?I0 )/(2I0 ?-?I)]?=?lgK?+?nlg[M], the binding constant K?=?6.84?×?10(6) ?L?mol(-1) and the number of binding sites (n)?=?0.76. The relative standard deviations were 3.2, 2.4 and 2.0% for 10.0, 20.0 and 30.0 ?pg ?mL(-1) Al(III) (n?=?7), respectively. This new method was successfully applied to continuous, quantitative monitoring of picogram level Al(III) in human saliva following oral intake of compound aluminum hydroxide tablets. It was found that Al(III) in saliva reached a maximum of 101.2? ng ?mL(-1) at 3.0?h. The absorption rate constant ka , elimination rate constant k and half-life time t1/2 of Al(III) were 1.378? h(-1) , 0.264? h(-1) and 2.624 ?h, respectively. PMID:24127408

  6. Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence.

    PubMed

    Liu, Wenwen; Xie, Liangxiao; Liu, Hongshuang; Xu, Shichao; Hu, Bingcheng; Cao, Wei

    2013-01-01

    A novel method for the detection of trace estradiol valerate (EV) in pharmaceutical preparations and human serum was developed by inhibition of luminol chemiluminescence (CL) by estradiol valerate on the zinc deuteroporphyrin (ZnDP)-enhanced luminol-K3 Fe(CN)6 chemiluminescence system. Under optimized experimental conditions, CL intensity and concentration of estradiol valerate had a good linear relationship in the ranges of 8.0 × 10(-8) to 1.0 × 10(-5) g/mL. Detection limit (3?) was estimated to be 3.5 × 10(-8) g/mL. The proposed method was applied successfully for the determination of estradiol valerate in pharmaceutical preparations and human serum and recoveries were 97.0-105.0% and 95.5-106.0%, respectively. The possible mechanism of the CL system is discussed. PMID:22777916

  7. Bio and chemiluminescence in bioanalysis

    Microsoft Academic Search

    A. Roda; P. Pasini; M. Guardigli; M. Baraldini; M. Musiani; M. Mirasoli

    2000-01-01

    Analytical chemiluminescence and bioluminescence represent a versatile, ultrasensitive tool with a wide range of applications\\u000a in diverse fields such as biotechnology, pharmacology, molecular biology, clinical and environmental chemistry. Enzyme activities\\u000a and enzyme substrates and inhibitors can be efficiently determined when directly involved in luminescent reactions, and also\\u000a when they take part in a reaction suitable for coupling to a final

  8. Fast gas chromotography with luminol detection for measurement of nitrogen dioxide and PANs.

    SciTech Connect

    Gaffney, J. S.; Marley, N. A.; Drayton, P. J.

    1999-09-30

    Fast capillary gas chromatography has been coupled to a luminol-based chemiluminescence detection system for the rapid monitoring of nitrogen dioxide and peroxyacyl nitrates. A first-generation instrument was described recently (Gaffney et al., 1998). This system is capable of monitoring nitrogen dioxide and peroxyacyl nitrates (PANs; to and including the C4 species) with 1-min time resolution. This is an improvement by a factor of five over gas chromatography methods with electron capture detection. In addition, the luminol method is substantially less expensive than laser fluorescent detection or mass spectroscopic methods. Applications in aircraft-based research have been published electronically and will appear shortly in Environmental Science and Technology (Gaffney et al., 1999a). An improved version of the instrument that has been designed and built makes use of a Hammamatsu photon-counting system. Detection limits of this instrumentation are at the low tens of ppt. The range of the instrument can be adjusted by modifying sampling volumes and detection counting times. A review of past work and of recent application of the instrumentation to field measurements of nitrogen dioxide and PANs is presented. The data clearly indicate that the luminol approach can determine the target species with time resolution of less than 1 min. Examples of applications for estimation of peroxyacetyl radical concentrations and nitrate radical formation rates are also presented. This instrumentation can further be used for evaluation of surfaces for loss of nitrogen dioxide and PANs, phenomena of possible importance for sampling interfaces and chamber wall design. Our high-frequency field data clearly indicate that the ''real world'' is not well mixed and that turbulent mixing and plume-edge chemistries might play an important role in urban- and regional-scale interactions. Dynamic flow systems might be required to evaluate such effects in new-generation chamber studies.

  9. Volume 5, number 4 CHEMICALPHYSICSLETTERS 1 April 1970 CROSSED BEAM CHEMILUMINESCENCE *

    E-print Network

    Zare, Richard N.

    Volume 5, number 4 CHEMICALPHYSICSLETTERS 1 April 1970 CROSSED BEAM CHEMILUMINESCENCE * Ch Chemiluminescent reactions are usually ob- served in relatively high-pressure sources, such as flames and flowing in an exothermic reaction. We repeat here a first study of mole- cular chemiluminescence obtained by crossing

  10. High chemiluminescence activity of an Fe(III)-TAML activator in aqueous-organic media and its use in the determination of organic peroxides.

    PubMed

    Demiyanova, Alexandra S; Sakharov, Ivan Yu

    2015-05-01

    High activity of Fe(III)-TAML, peroxidase mimic, upon the catalytic oxidation of luminol in aqueous-organic media (ethanol, isopropanol and acetonitrile) was determined. Using Fe(III)-TAML the sensitive chemiluminescence assays for the determination of benzoyl peroxide and tert-butyl hydroperoxide in the presence of organic solvents were performed. PMID:25782534

  11. Introducing novel amorphous carbon nanoparticles as energy acceptors into a chemiluminescence resonance energy transfer immunoassay system.

    PubMed

    Wang, Zhenxing; Gao, Hongfei; Fu, Zhifeng

    2013-11-21

    A novel chemiluminescence resonance energy transfer (CRET) system for competitive immunoassay of biomolecules was developed by using novel amorphous carbon nanoparticles (CNPs) prepared from candle soot as energy acceptors. The CNPs were firstly prepared to bind with the antigen (Ag) for obtaining the nanocomposite CNP-Ag, and this obtained CNP-Ag was then reacted with the horseradish peroxidase-labeled antibody (HRP-Ab) to assemble the CRET system. The luminol catalyzed by HRP serving as the energy donor for CNPs triggered the CRET phenomenon between luminol and CNPs, which led to the chemiluminescence signal decrease. Due to the competitive immunoreaction of the target antigen and the CNP-Ag, a part of the CNP-Ag was replaced from the HRP-Ab, and then resulted in a weaker interaction between luminol and CNPs. Thus the competitive immunoreaction led to a higher chemiluminescence emission. This CNP-based CRET system was successfully applied to detect the human IgG as a model analyte, and a linear range of 10-200 ng mL(-1) and a detection limit of 1.9 ng mL(-1) (S/N = 3) were obtained. The results for real sample analysis demonstrated its application potential in some important areas such as clinical diagnosis. PMID:23979821

  12. Determination of cytochrome c in human serum and pharmaceutical injections using flow injection chemiluminescence.

    PubMed

    Li, Xin; Liu, Houyong; He, Xili; Song, Zhenghua

    2010-02-01

    It was found that the complex of cytochrome c (Cyt c) and hydrogen peroxide could significantly catalyze the chemiluminescence (CL) reaction from luminol-hydrogen peroxide, and a sensitive, rapid, and simple CL procedure was proposed for the determination of Cyt c in a flow injection system for the first time. The increment of CL intensity was linear over the concentration of Cyt c ranging from 5 to 700 ng ml(-1), with a detection limit of 2 ng ml(-1) (3sigma). At a flow rate of 2.0 ml min(-1), a complete analytical process could be performed in 30 s with a relative standard deviation of less than 4.0%. The proposed method was applied successfully for the assay of Cyt c in pharmaceutical injections and human serum, and the recoveries were from 98.0% to 108.8% and 92.5% to 109.0%. The possible mechanism of Cyt c enhanced CL reaction was also discussed. PMID:19347266

  13. Study on the proteins-luminol binding by use of luminol as a fluorescence probe

    NASA Astrophysics Data System (ADS)

    He, Xili; Song, Zhenghua

    2013-10-01

    In this paper, a new mathematical equation of lg(F0 - F)/F = 1/nlg[P] + 1/nlgKa, which was used to obtain interaction parameters (the binding constant Ka and the number of binding sites n) between the protein and the small molecule ligand by using the ligand as a fluorescence (FL) probe, was constructed for the first time. The interaction parameters between myoglobin, catalase, lysozyme, bovine serum albumin (BSA) and luminol were obtained by this equation with luminol used as a FL probe, showing that the binding constants Ka were 8.78 × 105, 4.47 × 105, 4.21 × 104 and 3.95 × 104 respectively, and the number of binding sites n approximately equaled to 1.0 for myoglobin, catalase, and 2.0 for lysozyme, BSA. The interactions of ferritin, ovalbumin, aldolase, chymotrypsinogen and ribonuclease with luminol were also studied by this method. The binding constants Ka were at 104-105 level, and the number of binding sites n mostly approximately equaled to 2.0. The binding ability of luminol to the studied proteins followed the pattern: myoglobin > aldolase > ferritin > ovalbumin > catalase > ribonuclease > lysozyme > BSA > chymotrypsinoge.

  14. Dynamics of OH and OD radical reactions with HI and GeH4 as studied by infrared chemiluminescence of the H2O and HDO products

    NASA Astrophysics Data System (ADS)

    Butkovskaya, N. I.; Setser, D. W.

    1997-03-01

    The infrared chemiluminescence of vibrationally excited H2O and HDO from the highly exothermic reactions of OH and OD radicals with HI and GeH4 was observed in the 2200-5500 cm-1 range. The experiments utilized a fast-flow reactor with 0.3-1 Torr of Ar carrier gas at 300 K; the OH(OD) radicals were produced via the H(D)+NO2 reaction and the H or D atoms were generated by a discharge in a H2(D2)/Ar mixture. The H2O and HOD vibrational distributions were determined by computer simulation of the emission spectra in the 2200-3900 cm-1 range. The total vibrational energy released to H2O and HOD molecules is, respectively, =0.36 and 0.41 from HI and =0.46 and 0.51 from GeH4. These values are significantly smaller than for the reactions of OH and OD with HBr, =0.61 and 0.65. The populations of the O-H stretching vibration of HOD and the collisionally coupled ?1 and ?3 stretching modes of H2O decrease with increasing vibrational energy. In contrast, the vibrational distribution from the HBr reaction is inverted. The bending mode distributions in all stretching states of H2O and HOD extend to the thermodynamic limit of each reaction. A surprisal analysis was made for H2O(HOD) distributions from the title reactions and compared with that for OH(OD)+HBr. The surprisal analysis tends to confirm that the dynamics for the HI and GeH4 reactions differ from the HBr reaction. The HI reaction may proceed mainly via addition-migration, while the GeH4 reaction may involve both direct abstraction and addition-migration. A rate constant for the OH+GeH4?H2O+GeH3 reaction was evaluated by comparing the H2O emission intensities with that of the OH+HBr?H2O+Br reaction, kGeH4/kHBr=6.5±0.9. Secondary kinetic-isotope effects, kOH/kOD=1.4±0.1, 1.0±0.2, and 1.3±0.2, were determined for reactions of OH and OD with GeH4, HI, and HBr, respectively, by comparing the relative H2O and HOD emission intensities.

  15. Sensitive electrochemiluminescence detection for CA15-3 based on immobilizing luminol on dendrimer functionalized ZnO nanorods.

    PubMed

    Jiang, Xinya; Wang, Haijun; Yuan, Ruo; Chai, Yaqin

    2015-01-15

    In this study, we constructed a novel electrochemiluminescence (ECL) immunosensor for sensitive and selective detection of carbohydrate antigen 15-3 (CA15-3) by using polyamidoamine (PAMAM)-functionalized ZnO nanorods (ZNs-PAMAM) as carriers. PAMAM dendrimers with hyper-branched and three-dimensional structure were used as linked reagents for co-immobilization of luminol and CA15-3 detection antibody on the ZNs to prepare the signal probe. In addition, ZNs could hasten the decomposition of H2O2 to generate various reactive oxygen species (ROSs) which accelerated the ECL reaction of luminol with amplified ECL intensity. Compared with luminol in the detection solution, the ECL efficiencies of luminol could be improved by immobilizing luminol on the electrode due to the smaller distance between luminescence reagent and the electrode surface. Moreover, the electrodepositing gold nanoparticles (AuNPs) on the bare glass carbon electrode (GCE) with enhanced surface area could capture a large amount of primary anti-CA15-3 to improve the sensitivity of the immunosensor. Under the optimized experimental conditions, a wide linear range of 0.1-120?U?mL(-1) was acquired with a relatively low detection limit of 0.033?U?mL(-1) (S/N=3) for CA15-3. PMID:25051535

  16. Effects of luminol on the subsequent analysis of bloodstains.

    PubMed

    Laux, D L

    1991-09-01

    The effects of luminol upon additional presumptive chemical tests, subsequent confirmatory blood tests, species determination by immunoelectrophoresis, ABO typing by absorption elution, and genetic marker analysis by multienzyme system electrophoresis were examined. Results indicate that luminol does not affect additional presumptive chemical tests, confirmatory tests, species determination, or ABO typing, but does affect certain genetic marker systems. PMID:1835494

  17. Luminol as a fluorescent acid-base indicator.

    PubMed

    Erdey, L; Buzás, I; Vigh, K

    1966-03-01

    The acid and base dissociation constants of luminol are determined at various ionic strengths. The transition interval occurs at pH 7.7-9.0, therefore luminol is a fluorescent indicator for the titration of strong and weak acids and strong bases. Its value as an indicator is established by titrating milk, red wine and cherry juice. PMID:18959899

  18. Study of the H + O + M reaction forming OH{sup *}: Kinetics of OH{sup *} chemiluminescence in hydrogen combustion systems

    SciTech Connect

    Kathrotia, T.; Riedel, U. [Institute of Combustion Technology, German Aerospace Center (DLR) and University of Stuttgart, Stuttgart (Germany); Fikri, M.; Bozkurt, M.; Hartmann, M.; Schulz, C. [IVG, University of Duisburg-Essen, Duisburg (Germany)

    2010-07-15

    The temporal variation of OH{sup *} (A{sup 2}{sigma}{sup +}) chemiluminescence in hydrogen oxidation chemistry has been studied in a shock tube behind reflected shock waves at temperatures of 1400-3300 K and at a pressure of 1 bar. The aim of the present work is to obtain a validated reaction scheme to describe OH{sup *} formation in the H{sub 2}/O{sub 2} system. Temporal OH{sup *} emission profiles and ignition delay times for lean and stoichiometric H{sub 2}/O{sub 2} mixtures diluted in 97-98% argon were obtained from the shock-tube experiments. Based on a literature review for the hydrogen combustion system, the key reaction considered was H + O + M = OH{sup *} + M. The temperature dependence of the measured peak OH{sup *} emission from the shock tube and the peak OH{sup *} concentration from a homogeneous closed reactor model are compared. Based on these results a reaction rate coefficient of k{sub 1} = (1.5 {+-} 0.4) x 10{sup 13} exp(-25 kJ mol{sup -1}/RT) cm{sup 6} mol{sup -2} s{sup -1} was found for the forward reaction which is slightly higher than the rate coefficient suggested by Hidaka et al. (1982). The comparison of measured and simulated absolute concentrations shows good agreement. Additionally, a one-dimensional laminar premixed low-pressure flame calculation was performed for where absolute OH{sup *} concentration measurements have been reported by Smith et al. (2005). The absolute peak OH{sup *} concentration is fairly well reproduced if the above mentioned rate coefficient is used in the simulation. (author)

  19. Simple simultaneous determination of butylated hydroquinone (TBHQ) and butylated hydroxyanisole (BHA) antioxidants in oil using high-performance liquid chromatography with chemiluminescence detection.

    PubMed

    Xu, Shuangjiao; Chen, Funan; Deng, Mao; Sui, Yanyan

    2014-12-01

    A highly sensitive and convenient high-performance liquid chromatography technique coupled with chemiluminescence detection for the simultaneous determination butylated hydroquinone (TBHQ) and butylated hydroxyanisole (BHA) in oil is established. The detection is based on the inhibitory effect on the CL reaction between luminol and potassium ferricyanide in an alkaline medium. Samples were separated through a reverse-phase C18 column using a mobile phase of methanol and water (80: 20, v/v) at a flow rate of 0.5 mL/min. The effects of various parameters including mobile phase, flow rate and chemiluminescence regent were studied. Under optimum conditions, both TBHQ and BHA showed good linear relationships in the range 1 × 10(-7) -1 × 10(-5) g/mL with detection limits of 24 and 33 ng/mL, respectively. The proposed method is simple and sensitive, with low costs. The method was successfully applied for the quantification of TBHQ and BHA in sesame oil. The possible inhibition mechanism is also discussed briefly. PMID:24723449

  20. An ultrasensitive chemiluminescent method for the tanshinol borneol ester determination and its pharmacokinetic analysis.

    PubMed

    Nie, Fei; Bu, Min; Wu, Lihong; Zheng, Jianbin

    2014-03-01

    Tanshinol borneol ester (DBZ), a chemical combination of danshensu and borneol, is an experimental drug that exhibits efficacious anti-ischemic activity in animal models. In this work, an ultrasensitive chemiluminescence (CL) method for the determination of DBZ was established based on the inhibitory effect of DBZ on the CL signal produced from the reaction between potassium permanganate and luminol in alkaline solution. The CL intensity responded linearly to the concentration of DBZ in the range 2.0 × 10(-10) to 4.0 × 10(-8) g/mL with a detection limit of 7 × 10(-11) g/mL. The relative standard deviation (RSD) was 3.8% for 4.0 × 10(-9) g DBZ (n = 11). The proposed method showed characteristics of high sensitivity, simple device and quick. In addition, this proposed method had been applied satisfactorily to the analysis of DBZ in blood. The pharmacokinetics of DBZ in rat has also been studied using the CL method. PMID:23681963

  1. Recent Advances in Chemiluminescence

    Microsoft Academic Search

    Yingying Su; He Chen; Zhimeng Wang; Yi Lv

    2007-01-01

    This article reviews the development and application of chemiluminescence (CL) published in the literature between January 2004 and October 2006, with regard to instrumentation, systems, applications, and conclusions.

  2. A hot-spot-active magnetic graphene oxide substrate for microRNA detection based on cascaded chemiluminescence resonance energy transfer

    NASA Astrophysics Data System (ADS)

    Bi, Sai; Chen, Min; Jia, Xiaoqiang; Dong, Ying

    2015-02-01

    Herein, a cascaded chemiluminescence resonance energy transfer (C-CRET) process was demonstrated from horseradish peroxidase (HRP)-mimicking DNAzyme-catalyzed luminol-H2O2 to fluorescein and further to graphene oxide (GO) when HRP-mimicking DNAzyme/fluorescein was in close proximity to the GO surface. The proposed C-CRET system was successfully implemented to construct three modes of C-CRET hot-spot-active substrates (modes I, II and III) by covalently immobilizing HRP-mimicking DNAzyme/fluorescein-labeled hairpin DNAs (hot-spot-generation probes) on magnetic GO (MGO), resulting in a signal ``off'' state due to the quenching of the luminol/H2O2/HRP-mimicking DNAzyme/fluorescein CRET system by GO. Upon the introduction of microRNA-122 (miRNA-122), the targets (mode I) or the new triggers that were generated through a strand displacement reaction (SDR) initiated by miRNA-122 (modes II and III) hybridized with the loop domains of hairpin probes on MGO to form double-stranded (modes I and II) or triplex-stem structures (mode III), causing an ``open'' configuration of the hairpin probe and a CRET signal ``on'' state, thus achieving sensitive and selective detection of miRNA-122. More importantly, the substrate exhibited excellent controllability, reversibility and reproducibility through SDR and magnetic separation (modes II and III), especially sequence-independence for hairpin probes in mode III, holding great potential for the development of a versatile platform for optical biosensing.Herein, a cascaded chemiluminescence resonance energy transfer (C-CRET) process was demonstrated from horseradish peroxidase (HRP)-mimicking DNAzyme-catalyzed luminol-H2O2 to fluorescein and further to graphene oxide (GO) when HRP-mimicking DNAzyme/fluorescein was in close proximity to the GO surface. The proposed C-CRET system was successfully implemented to construct three modes of C-CRET hot-spot-active substrates (modes I, II and III) by covalently immobilizing HRP-mimicking DNAzyme/fluorescein-labeled hairpin DNAs (hot-spot-generation probes) on magnetic GO (MGO), resulting in a signal ``off'' state due to the quenching of the luminol/H2O2/HRP-mimicking DNAzyme/fluorescein CRET system by GO. Upon the introduction of microRNA-122 (miRNA-122), the targets (mode I) or the new triggers that were generated through a strand displacement reaction (SDR) initiated by miRNA-122 (modes II and III) hybridized with the loop domains of hairpin probes on MGO to form double-stranded (modes I and II) or triplex-stem structures (mode III), causing an ``open'' configuration of the hairpin probe and a CRET signal ``on'' state, thus achieving sensitive and selective detection of miRNA-122. More importantly, the substrate exhibited excellent controllability, reversibility and reproducibility through SDR and magnetic separation (modes II and III), especially sequence-independence for hairpin probes in mode III, holding great potential for the development of a versatile platform for optical biosensing. Electronic supplementary information (ESI) available: Sequences of RNA and DNA used in this study, relationship of the proposed three modes, CRET mechanism of the luminol/H2O2/HRP-mimicking DNAzyme/fluorescein system, calculation of the surface coverage of hairpin probe I-1 on MGO, control experiment, comparison between different modes for microRNA detection, and advantages of the proposed strategy. See DOI: 10.1039/c4nr06603k

  3. MOBILE SOURCE NOX MONITOR, HYDROGEN-ATOM DIRECT CHEMILUMINESCENCE METHOD

    EPA Science Inventory

    An analyzer was developed for measuring motor vehicle NOx (NO and NO2) emissions based on the chemiluminescence reaction of NO and NO2 with hydrogen atoms. This eliminated the need for an NO2 to NO converter as required with ozone chemiluminescence for NOx analysis. The hydrogen-...

  4. Flow-injection chemiluminescence determination of tryptophan through its peroxidation and epoxidation by peroxynitrous acid

    Microsoft Academic Search

    Yao-Dong Liang; Jun-Feng Song

    2005-01-01

    A flow-injection chemiluminescence method for the determination of tryptophan was proposed, which was based on an intense chemiluminescence of tryptophan in hydrogen peroxide–nitrite–sulfuric acid medium. The chemiluminescence reaction was attributed to peroxidation and epoxidation of tryptophan by peroxynitrous acid, and subsequent decomposition of the formed dioxetane. The chemiluminescence intensity was linear with tryptophan in the range of 6.0×10?7 to 3.0×10?5moll?1

  5. Sensitive and selective determination of fluvoxamine maleate using a sensitive chemiluminescence system based on the alkaline permanganate-Rhodamine B-gold nanoparticles reaction.

    PubMed

    Hassanzadeh, Javad; Amjadi, Mohammad

    2015-06-01

    A high-yield chemiluminescence (CL) system based on the alkaline permanganate-Rhodamine B reaction was developed for the sensitive determination of fluvoxamine maleate (Flu). Rhodamine B is oxidized by alkaline KMnO4 and a weak CL emission is produced. It was demonstrated that gold nanoparticles greatly enhance this CL emission due to their interaction with Rhodamine B molecules. It is also observed that sodium dodecyl sulfate, an anionic surfactant, can strongly increase this enhancement. In addition, it was demonstrated that a notable decrease in the CL intensity is observed in the presence of Flu. This may be related to Flu oxidation with KMnO4 . There is a linear relationship between the decrease in CL intensity and the Flu concentration over a range of 2-300 µg/L. A new simple, rapid and sensitive CL method was developed for the determination of Flu with a detection limit (3s) of 1.35 µg/L. The proposed method was used for the determination of Flu in pharmaceutical and urine samples. Copyright © 2014 John Wiley & Sons, Ltd. PMID:25214010

  6. Sensitive determination of positional isomers of benzenediols in human urine by boronate affinity capillary electrophoresis with chemiluminescence detection.

    PubMed

    Lin, Zian; Sun, Xiaobo; Hu, Wenli; Yin, Yuqing; Chen, Guonan

    2014-04-01

    A boronate ACE coupled with chemiluminescence (CL) detection was developed for sensitive determination of three isomeric benzenediols, which was based on the principle of an inhibited effect of borate complexation on the CL reaction between luminol and potassium hexacyanoferrate (K3 Fe(CN)6 ) in alkaline solution. The effects of some important factors on CE separation and CL intensity were systemically investigated. Baseline separation of isomeric benzenediols including o-benzenediol, m-benzenediol, and p-benzenediol was achieved by using a mobile phase of 40 mmol/L glycine-NaOH buffer at pH 9.4 containing 0.8 mmol/L luminol and 0.4 mol/L 4-iodophenylboronic acid. The calibration curves of the analytes by plotting the peak height against corresponding concentration were linear over the range of 4.5 × 10(-8) ? 4.5 × 10(-5) mol/L for p-benzenediol, 6.8 × 10(-8) ? 2.7 × 10(-5) mol/L for m-benzenediol, and 9.0 × 10(-8) ? 4.5 × 10(-5) mol/L for o-benzenediol. The corresponding detection limits for p-, m-, and o-benzenediols were 2.8 × 10(-8) mol/L (68 amol), 3.2 × 10(-8) mol/L (108.4 amol), and 3.7 × 10(-8) mol/L (125.8 amol; S/N = 3), respectively. The proposed method has been successfully applied to the analysis of trace benzenediols in spiked human urine sample and the recoveries were >97.2%. Our primary result demonstrated the proposed CE-CL method has great potential for biomarker determination in clinical diagnosis. PMID:24115126

  7. FI automatic method for the determination of copper(II) based on coproporphyrin I-Cu(II)/TCPO/H(2)O(2) chemiluminescence reaction for the screening of waters.

    PubMed

    Meseguer-Lloret, S; Campíns-Falcó, P; Cárdenas, S; Gallego, M; Valcárcel, M

    2004-11-15

    In this paper, an automatic method for the screening of water samples containing Cu(II) was proposed, based on peryoxalate chemiluminescence reaction using coproporphyrin I as fluorophor compound to provide selectivity and a simple flow injection (FI) chemiluminescence detector (CLD). FI system conditions were chosen in order to distinguish samples over or under legislation limit established (50mugl(-1)) with high reliability. The detection limit found was 9mugl(-1) and the linear dynamic range was 15-125mugl(-1) of Cu(II). Repeatibility and reproducibility studies gave good precision and accuracy with recovery near 100%. Under these conditions, the method resulted selective and only Fe(II), Fe(III) and Pb(II) could interfere, but at a concentration level higher than their normal concentration in waters. The proposed method was found to be highly reliable for screening purposes and it was successfully applied to the screening of a variety of real water samples. PMID:18969707

  8. Aqueous chemiluminescent systems

    NASA Technical Reports Server (NTRS)

    Mohan, Arthur Gaudens (Inventor)

    1977-01-01

    This invention relates to novel water-soluble esters of oxalic acid, and to compositions that are useful for generating chemiluminescent emission by reacting said esters of oxalic acid with hydrogen peroxide in the presence of water and a fluorescent compound, and to a process for generating chemiluminescent emission by using said compositions.

  9. Determination of Hydrogen Peroxide in Water by Chemiluminescence Detection, (I) Flow Injection Type Hydrogen Peroxide Detection System

    Microsoft Academic Search

    Naoya YAMASHIRO; Shunsuke UCHIDA; Yoshiyuki SATOH; Yusuke MORISHIMA; Hiroaki YOKOYAMA; Tomonori SATOH; Junichi SUGAMA; Rie YAMADA

    2004-01-01

    The photocurrent detected by the detection system is expressed by a linear function of the hydrogen peroxide concen- tration in the region of lower concentration ((H2O2)<10 ppb), while it is expressed by a quadratic function of (H2O2) in the region of higher concentration ((H2O2)>10 ppb). The luminous intensity of luminol chemiluminescence is the highest when pH of the reagent mixture

  10. Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schro?dinger equation.

    PubMed

    Greenman, Loren; Mazziotti, David A

    2011-05-01

    Direct computation of energies and two-electron reduced density matrices (2-RDMs) from the anti-Hermitian contracted Schro?dinger equation (ACSE) [D. A. Mazziotti, Phys. Rev. Lett. 97, 143002 (2006)], it is shown, recovers both single- and multi-reference electron correlation in the chemiluminescent reaction of dioxetanone especially in the vicinity of the conical intersection where strong correlation is important. Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical intersection. Our previous active-space 2-RDM study of dioxetanone [L. Greenman and D. A. Mazziotti, J. Chem. Phys. 133, 164110 (2010)] concluded that correlating 16 electrons in 13 (active) orbitals is required for realistic surfaces without correlating the remaining (inactive) orbitals. In this paper we pursue two complementary goals: (i) to correlate the inactive orbitals in 2-RDMs along dioxetanone's reaction coordinate and compare these results with those from multireference second-order perturbation theory (MRPT2) and (ii) to assess the size of the active space-the number of correlated electrons and orbitals-required by both MRPT2 and ACSE for accurate energies and surfaces. While MRPT2 recovers very different amounts of correlation with (4,4) and (16,13) active spaces, the ACSE obtains a similar amount of correlation energy with either active space. Nevertheless, subtle differences in excitation energies near the conical intersection suggest that the (16,13) active space is necessary to determine both energetic details and properties. Strong electron correlation is further assessed through several RDM-based metrics including (i) total and relative energies, (ii) the von Neumann entropy based on the 1-electron RDM, as well as the (iii) infinity and (iv) squared Frobenius norms based on the cumulant 2-RDM. PMID:21548676

  11. Chemiluminescence involving acidic and ambient ion light emitters. The chemiluminescence of the 9-acridinepercarboxylate anion

    SciTech Connect

    White, E.H.; Roswell, D.F.; Dupont, A.C.; Wilson, A.A.

    1987-08-19

    The reaction of phenyl 9-acridinecarboxylate with an excess of peroxide ion in THF/water (67/33 mol %) leads to the emission of either bright yellow-green light or bright blue light, depending on the reaction conditions. The blue emission is favored by high concentrations of hydrogen peroxide and water, for example. 9-Acridinepercarboxylic acid is a common intermediate in the reactions. The light emitter responsible for the blue chemiluminescence is acridone, whereas that responsible for the yellow-green chemiluminescence is the anion of acridone. The effects of base concentration and solvent composition on the relative proportions of these two emitters have produced evidence that, contrary to the expectation of simple theory, a dioxetanone is not an intermediate in the reaction. Other cases where chemiluminescence may involve percarboxylate and peroxide ions are discussed.

  12. Chemiluminescent spectra of alkali-halogen reactions R. C. Oldenbarg, J. L. Gale*, and R. N. Zare

    E-print Network

    Zare, Richard N.

    and those of flame studies are noted and rationalized in terms of the different effective rotational the conditions prevailing in flame studies. For the mixed halogen reactions, the MY ground state is the more stable salt molecule that can be formed from the halogen atoms of the XY pair. A four-center mechanism

  13. Tested Demonstrations. A Chemiluminescence Demonstration - Oxalyl Chloride Oxidation.

    ERIC Educational Resources Information Center

    Gilber, George L., Ed.

    1979-01-01

    This inexpensive, effective chemiluminescence demonstration requires minimal preparation. It is based on the oxidation of oxalyl chloride by hydrogen peroxide in the presence of an appropriate fluorescent sensitizer. The reaction mechanism is not completely understood. (BB)

  14. A Chemiluminescence Detector for Ozone Measurement.

    ERIC Educational Resources Information Center

    Carroll, H.; And Others

    An ozone detector was built and evaluated for its applicability in smog chamber studies. The detection method is based on reaction of ozone with ethylene and measurement of resultant chemiluminescence. In the first phase of evaluation, the detector's response to ozone was studied as a function of several instrument parameters, and optimum…

  15. Chemiluminescent Detection of Diffusion

    NSDL National Science Digital Library

    Evelyn Bradshaw (Cleveland Heights High School)

    1995-07-01

    This activity engages students in observing the results of diffusion through a selectively permeable membrane, monitoring the concentration of solute in water, learning the simple test for starch, and quantitating the chemiluminescence by exposure to photographic paper.

  16. Flow injection chemiluminescence determination of sudan I in hot chilli sauce.

    PubMed

    Liu, Yanhong; Song, Zhenghua; Dong, Faxin; Zhang, Lin

    2007-02-01

    A chemiluminescence method based on the luminol-H2O2 system with flow injection technology was proposed for the determination of sudan I in hot chilli sauce. It was found that sudan I could enhance chemiluminescence intensity generated from the luminol-H2O2 system. The increment of chemiluminescence intensity was proportional to the concentration of sudan I, giving a calibration graph linear over the concentration from 10 pg mL-1 to 7 ng mL-1 (R 2 = 0.9980) with the detection limit of 3 pg mL-1 (3sigma) and the quantification limit of 7.5 pg mL-1. At a flow rate of 2.0 mL min-1, one analysis cycle, including sampling and washing, could be accomplished in 60 s with a relative standard deviation of <5.0%. The method has been applied successfully to the determination of sudan I in Pixian douban, Golden Mark guilin chilli sauce, and Golden Mark satay sauce, and the recovery was 90.6-110.0%. PMID:17263450

  17. Quality Control of Reactive Oxygen Species Measurement by Luminol-Dependent Chemiluminescence Assay

    Microsoft Academic Search

    HIROSHI KOBAYASHI; ENRIQUE GIL-GUZMAN; AYMAN M. MAHRAN; RAKESH K. SHARMA; DAVID R. NELSON; ANTHONY J. THOMAS JR; ASHOK AGARWAL

    A total of 28 donor semen samples were used to eval- uate the characteristics of laboratory variability in measuring reactive oxygen species (ROS). The objectives of this study were to assess the interassay (same sample observed on different days by the same observers) variability; interdonor, intraobserver (replications of the same sample on the same day) variability; and interobserver (multiple observers

  18. Chemi-luminescence measurements of hyperthermal Xe{sup +}/Xe{sup 2+}+ NH{sub 3} reactions

    SciTech Connect

    Prince, Benjamin D.; Steiner, Colby P. [Air Force Research Laboratory, Space Vehicles Directorate, Kirtland AFB, New Mexico 87117 (United States); Chiu, Yu-Hui [Busek Co. Inc, Natick, Massachusetts 01760 (United States)

    2012-04-14

    Luminescence spectra are recorded for the reactions of Xe{sup +}+ NH{sub 3} and Xe{sup 2+}+ NH{sub 3} at energies ranging from 11.5 to 206 eV in the center-of-mass (E{sub cm}) frame. Intense features of the luminescence spectra are attributed to the NH (A {sup 3}{Pi}{sub i}-X {sup 3}{Sigma}{sup -}), hydrogen Balmer series, and Xe I emission observable for both primary ions. Evidence for charge transfer products is only found through Xe I emission for both primary ions and NH{sup +} emission for Xe{sup 2+} primary ions. For both primary ions, the absolute NH (A-X) cross section increases with collision energy before leveling off at a constant value, approximately 9 x 10{sup -18} cm{sup 2}, at about 50 eV while H-{alpha} emission increases linearly with collision energy. The nascent NH (A) populations derived from the spectral analysis are found to be independent of collision energy and have a constant rotational temperature of 4200 K.

  19. Flow-injection chemiluminescence sensor for determination of isoniazid in urine sample based on molecularly imprinted polymer

    NASA Astrophysics Data System (ADS)

    Xiong, Yan; Zhou, Houjiang; Zhang, Zhujun; He, Deyong; He, Chao

    2007-02-01

    In this paper, molecularly imprinted polymer (MIP) of isoniazid is synthesized through thermal radical copolymerization of metharylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of isoniazid template molecules. A novel flow injection chemiluminescence sensor for isoniazid determination is developed by packing the isoniazid-MIP into the flow cell as recognition elements. Isoniazid could be selectively adsorbed by the MIPs and the adsorbed isoniazid was sensed by its great enhancing effect on the weak CL reaction between luminol and periodate which were mixed in the flow cell. The enhanced CL intensity is linear in the range 2 × 10 -9 to 2 × 10 -7 g/mL and the detection limit is 7 × 10 -10 g/mL (3 ?) isoniazid with a relative standard deviation 2.8% ( n = 9) for 8 × 10 -8 g/mL. The sensor is reversible and reusable. It has a great improvement in sensitivity and selectivity for CL analysis. As a result, the sensor has been successfully applied to determination of isoniazid in human urine. At the same time, the binding characteristic of the polymer to isoniazid was evaluated by batch method and the dynamic method, respectively.

  20. Chemiluminescents Light Up the Night.

    ERIC Educational Resources Information Center

    Dashiell, Judy

    1997-01-01

    Provides a general description of chemiluminescence and distinguishes between the two types of chemistry that contribute to our understanding of chemiluminescence: fluorescence and the excitation process. Presents an activity that explores the phenomenon. (DDR)

  1. A luminol-based micro-flow-injection electrochemiluminescent system to determine reactive oxygen species.

    PubMed

    Chen, Ming; Wei, Xiuhua; Tu, Yifeng

    2011-09-15

    A flow injection analysis (FIA) system with electrochemiluminescent (ECL) detection has been established. Based on a specially designed flow-through ECL cell with a very simple structure, the system possesses rapid response and high sensitivity. With luminol as the ECL reagent, the response of hydrogen peroxide (H(2)O(2)) was investigated on the developed FIA-ECL system. After optimizing the experimental conditions, such as the electric parameters, the buffer condition and the flow rate, it was demonstrated that the developed FIA-ECL system works well for quantified assays. Compared with reported works, the present results indicate that the developed FIA-ECL system has the lowest limit of detection (S/N=3) of 3.0×10(-9) mol/L for H(2)O(2), which is equal to the level of chemiluminescence (CL). The developed system was successfully used to monitor the yield of reactive oxygen species (ROSs) in water vapour during the work of an ultrasonic humidifier with H(2)O(2) as index. And the amount of ROSs in some other real samples, including tap water, drinking water and river water was detected with recoveries from 92.0% to 106%. PMID:21807187

  2. Chemiluminescence detectors for liquid chromatography.

    PubMed

    Adcock, Jacqui L; Terry, Jessica M; Barrow, Colin J; Barnett, Neil W; Olson, Don C; Francis, Paul S

    2011-03-01

    In this tutorial we describe the construction of chemiluminescence detectors for high performance liquid chromatography (HPLC), comprising the components required to deliver the chemiluminescence reagent, a coiled-tubing flow cell, photomultiplier tube and detector housing, and various options for data acquisition. We also discuss two state-of-the-art commercially available chemiluminescence detectors for HPLC and other flow analysis methodology. PMID:21425470

  3. Chemiluminescent screening of quenching effects of natural colorants against reactive oxygen species: Evaluation of grape seed, monascus, gardenia and red radish extracts as multi-functional food additives

    Microsoft Academic Search

    M. Wada; H. Kido; K. Ohyama; T. Ichibangase; N. Kishikawa; Y. Ohba; M. N. Nakashima; N. Kuroda; K. Nakashima

    2007-01-01

    The quenching effects of commercial available natural colorants against reactive oxygen species (ROS) were investigated. The effectiveness was evaluated by measuring the quenching ratio of chemiluminescence (CL) intensity with each colorant on luminol or methyl-6-(p-methoxyphenyl)-3,7-dihydroimidaz[1,2-a]pyrazin-3-one CL induced by ROS. As a result, the grape seed extracts and polyphenolics dose-dependently quenched CL. The quenching effects of grape seed extract A at

  4. Ultrasensitive Study of Gatifloxacin Based on Its Enhancing Effect on the Cerium (IV)Sodium Hyposulfite Chemiluminescence Reaction in a Micellar Medium

    Microsoft Academic Search

    Mohammad Kamruzzaman; Al-Mahmnur Alam; Taslima Ferdous; Sang Hak Lee; Young Ho Kim; Sung Hong Kim

    A sensitive and rapid flow-injection chemiluminescence (CL) method has been developed for the determination of gatifloxacin\\u000a in pharmaceutical preparations and biological samples. The method is based on the enhancing effect of gatifloxacin on CL emission\\u000a generated by the interaction of Ce (IV) in sulphuric acid and sodium hyposulphite (Na2S2O4) sensitized by sodium dodecyl benzene sulfonate (SDBS). Strong CL emission was

  5. Label-free chemiluminescent aptasensor for platelet-derived growth factor detection based on exonuclease-assisted cascade autocatalytic recycling amplification.

    PubMed

    Bi, Sai; Luo, Baoyu; Ye, Jiayan; Wang, Zonghua

    2014-12-15

    Here an exonuclease III (Exo III)-assisted cascade autocatalytic recycling amplification (Exo-CARA) strategy is proposed for label-free chemiluminescent (CL) detection of platelet-derived growth factor BB (PDGF-BB) by taking advantage of both recognition property of aptamer and cleavage function of Exo III. Functionally, this system consists of a duplex DNA (aptamer-blocker hybrid), two kinds of hairpin structures (MB1 and MB2), and Exo III. Upon recognizing and binding with PDGF-BB, aptamer folds into a close configuration, which initiates the proposed Exo-CARA reaction (Recyclings I?II?III?II). Finally, numerous "caged" G-quadruplex sequences on DNAzyme1 and DNAzyme2 release that intercalate hemin to catalyze the oxidation of luminol by H2O2 to generate an amplified CL signal, achieving excellent specificity and high sensitivity with a detection limit of 6.8×10(-13) M PDGF-BB. The proposed strategy has the advantages of simple design, isothermal conditions, homogeneous reaction without separation and washing steps, effective-cost without the need of labeling, and high amplification efficiency, which might be a universal and promising protocol for the detection of a variety of biomolecules whose aptamers undergo similar conformational changes. PMID:25016251

  6. Improved method for chemiluminescent determination of peroxidase-mimicking DNAzyme activity.

    PubMed

    Gribas, Anastasia V; Zhao, Shulin; Sakharov, Ivan Yu

    2014-12-01

    The optimization of experimental conditions for the chemiluminescent determination of peroxidase-mimicking DNAzyme (PMDNAzyme) formed at the interaction of hemin and its aptamer EAD2 was performed. The effect of concentrations of hydrogen peroxide and luminol, acidity of the substrate solution, and composition and concentration of the assay buffer was estimated. Under optimized conditions, a value of detection limit for the PMDNAzyme was 350 pM. A comparison of the conditions determined in this work with those reported previously showed that the optimization of the composition of the substrate solution improved the sensitivity of the chemiluminescent determination of the PMDNAzyme. The obtained results open up promising perspectives for using the proposed method to improve the sensitivity of PMDNAzyme-based assays. PMID:25150107

  7. Sensitive competitive flow injection chemiluminescence immunoassay for IgG using gold nanoparticle as label

    NASA Astrophysics Data System (ADS)

    Qi, Honglan; Shangguan, Li; Liang, Lin; Ling, Chen; Gao, Qiang; Zhang, Chengxiao

    2011-11-01

    A sensitive competitive flow injection chemiluminescence (CL-FIA) immunoassay for immunoglobulin G (IgG) was developed using gold nanoparticle as CL label. In the configuration, anti-IgG antibody was immobilized on a glass capillary column surface by 3-(aminopropyl)-triethoxysilane and glutaraldehyde to form immunoaffinity column. Analyte IgG and gold nanoparticle labeled IgG were passed through the immunoaffinity column mounted in a flow system and competed for the surface-confined anti-IgG antibody. CL emission was generated from the reaction between luminol and hydrogen peroxide in the presence of Au (III), generated from chemically oxidative dissolution of gold nanoparticle by an injection of 0.10 mol L -1 HCl-0.10 mol L -1 NaCl solution containing 0.10 mmol L -1 Br 2. The concentration of analyte IgG was inversely related to the amount of bound gold nanoparticle labeled IgG and the CL intensity was linear with the concentration of analyte IgG from 1.0 ng mL -1 to 40 ng mL -1 with a detection limit of 5.2 × 10 -10 g mL -1. The whole assay time including the injections and washing steps was only 30 min for one sample, which was competitive with CL immunoassays based on a gold nanoparticle label and magnetic separation. This work demonstrates that the CL immunoassay incorporation of nanoparticle label and flow injection is promising for clinical assay with sensitivity and high-speed.

  8. Determination of residual enrofloxacin in food samples by a sensitive method of chemiluminescence enzyme immunoassay.

    PubMed

    Yu, Fei; Yu, Songcheng; Yu, Lanlan; Li, Yanqiang; Wu, Yongjun; Zhang, Hongquan; Qu, Lingbo; Harrington, Peter de B

    2014-04-15

    A chemiluminescence enzyme immunoassay (CLEIA) based on the HRP-luminol-H?O? chemiluminescence system for highly sensitive detection of enrofloxacin (ENR) was proposed in this study. Key factors that affect the precision and accuracy for the determination of ENR residues were optimised. Under the optimal conditions, the proposed method showed an excellent performance. The linearity range for method developed for determination of ENR was 0.35-1.0 ng/mL with a correlation coefficient greater than 0.994. The limit of detection was 0.03 ng/mL and the relative standard deviations (RSDs) were less than 9.4% and 13.0% for intra-day and inter-day assays. The proposed method was satisfactorily applied to determine ENR in milk, eggs, and honey samples at three spiked levels (0.4, 0.7, and 1.0 ng/mL) and the recoveries ranged from 92.4% to 104.2% for milk, 93.8% to 103.2% for eggs and 94.1% to 105.0% for honey, respectively. Compared the results of CLEIA with those of ELISA and HPLC, the advantages of the CLEIA were further confirmed. Moreover, one 96-well microtiter plate coated with anti-ENR can be used to detect multiple samples at the same time, which indicated that the CLEIA using HRP-luminol-H?O? system was a sensitive, high throughput and real-time method for ENR residues analysis. PMID:24295678

  9. Electrogenerated chemiluminescence from violanthrone.

    PubMed

    Quickenden, T I; Hansongnern, K

    1995-01-01

    Violanthrone, an emitter of exceptionally bright chemiluminescence, was examined in dimethylformamide solution to determine whether it also emits particularly bright electrogenerated chemiluminescence (ECL). The ECL measurements were carried out using a cycled potential which was applied to platinum electrodes. At the maximum sweep rate of 80 V s-1 available, the intensity of the violanthrone ECL was still increasing with sweep rate and was c. 56% of that from rubrene, a bright, commonly used emitter of ECL. Furthermore, assuming that the emission arises from radical anion-cation recombination, the sweep rate dependence showed that the least stable radical ion (probably the cation) decays with a half-life shorter than 0.2 s. PMID:7676850

  10. Determination of naphazoline hydrochloride in biological and pharmaceutical samples by a quantum dot-assisted chemiluminescence system using response-surface methodology.

    PubMed

    Imani-Nabiyyi, Amin; Sorouraddin, Mohammad H

    2014-12-01

    A simple and highly sensitive chemiluminescence (CL) method is reported for the determination of naphazoline hydrochloride (NH). It was found that the weak CL from the reaction of luminol and KIO4 in an alkaline medium could be highly amplified by cysteine-capped cadmium telluride quantum dots (QDs) and the enhanced CL was effectively quenched by NH and this finding was utilized as a basis for the determination of NH. The QDs were synthesized in aqueous medium and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and UV-vis and photoluminescence spectroscopy. A possible mechanism was proposed for the CL system based on radical identification experiments, along with CL spectrum of the system. The experimental parameters were optimized by the reliable response surface methodology (RSM). Under the optimized experimental conditions, the proposed method allowed the determination of NH over the range of 5.0 × 10(-10) -2.0 × 10(-7) mol/L (r(2) = 0.9993, n = 10). The precision (RSD%) of the method, obtained from five replicate determinations of 2.0 and 150 nmol/L NH, was found to be 1.0% and 1.3%, respectively. The method was successfully applied to the determination of NH in pharmaceutical formulations and human urine and serum samples with results corroborated with the aid of those obtained from a standard method. PMID:24733642

  11. Real-time monitoring of picomolar concentrations of iron(II) in marine waters using automated flow injection-chemiluminescence instrumentation.

    PubMed

    Bowie, Andrew R; Achterberg, Eric P; Sedwick, Peter N; Ussher, Simon; Worsfold, Paul J

    2002-11-01

    A shipboard-deployable, flow-injection (FI) based instrument for monitoring iron(II) in surface marine waters is described. It incorporates a miniature, low-power photon-counting head for measuring the light emitted from the iron(II)-catalyzed chemiluminescence (CL) luminol reaction. System control, signal acquisition, and data processing are performed in a graphical programming environment. The limit of detection for iron(II) is in the range 8-12 pmol L(-1) (based on 3 s of the blank), and the precision over the range 8-1000 pmol L(-1) varies between 0.9 and 7.6% (n = 4). Results from a day-night deployment during a north-to-south transect of the Atlantic Ocean and a daytime transect in the Sub-Antarctic Front are presented together with ancillary temperature, salinity, and irradiance data. The generic nature of the components used to assemble the instrument make the technology readily transferable to other laboratories and the modular construction makes it easy to adapt the system for use with other CL chemistries. PMID:12433170

  12. "Signal on" electrochemiluminescence pentachlorophenol sensor based on luminol-MWCNTs@graphene oxide nanoribbons system.

    PubMed

    Liu, Qian; Huan, Juan; Fei, Airong; Mao, Hanping; Wang, Kun

    2015-03-01

    A "signal on" electrochemiluminescence (ECL) sensor for pentachlorophenol (PCP) detection was constructed based on the amplified ECL of luminol at a multiwalled carbon nanotubes@graphene oxide nanoribbons (MWCNTs@GONRs) modified electrode. Due to the good electrocatalytic activity of MWCNTs@GONRs toward luminol system, the oxidation peak current of luminol at the MWCNTs@GONRs modified electrode was enhanced for ~6-fold than that of the bare electrode; and the ECL intensity of luminol was amplified for ~5.3-fold correspondingly. Furthermore, the amplified ECL signal of luminol was linear with the concentration of PCP in the range between 2pgmL(-1) and 10ngmL(-1) with a detection limit of 0.7pgmL(-1) (S/N=3). With the merits of good reproducibility, acceptable stability, wide linear range, low detection limit and simplicity, the proposed luminol ECL sensor showed great potential in the field of analytical applications. PMID:25618692

  13. A review of recent trends in analytical applications of electrogenerated chemiluminescence

    Microsoft Academic Search

    Andrew W Knight

    1999-01-01

    The development of analytical applications involving the electrochemical generation of chemiluminescence (ECL) in the last 5 years is reviewed. The mechanisms of common ECL reactions are summarised, and the potential advantages of ECL over conventional chemiluminescence are discussed. The current limitations of the technique are considered along with how they are being addressed. Finally some pointers as to likely directions

  14. Sensitive and selective determination of glucose in human serum and urine based on the peroxyoxalate chemiluminescence reaction of a new Fluorophore

    NASA Astrophysics Data System (ADS)

    Zargoosh, Kiomars; Shamsipur, Mojtaba; Qandalee, Mohammad; Piltan, Mohammad; Moradi, Loghman

    2011-10-01

    A novel method for simple and sensitive determination of glucose based on the peroxyoxalate chemiluminescence (PO-CL) detection of enzymatically generated H 2O 2 was investigated. Influence of various experimental parameters on glucose sensing, including the action time of the enzyme, solution pH, interferents and the concentration of CL reagents was investigated. Under the optimum condition, the linear response range of glucose was found to be 2.50 × 10 -6 to 1.75 × 10 -4 mol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) was 1.10 × 10 -6 mol/L. The present method has been used to determine the glucose concentrations in real serum and urine samples with satisfactory results.

  15. Modulatory effect of visible light on chemiluminescence of stimulated and nonstimulated blood leukocytes of carp (Cyprinus carpio, L)

    NASA Astrophysics Data System (ADS)

    Belotsky, Sandro; Avtalion, Ramy R.; Friedmann, Harry; Lubart, Rachel

    1998-12-01

    Irradiation of carp blood leukocytes with a non-laser visible light resulted in a significant inhibition of the spontaneous luminol-dependent chemiluminescence in the cells of a part of the fish. Those leukocytes that were sensitive to the visible light, showed a shorter time-to-peak than the non sensitive, following their stimulation with Ca ionophore. Because a shorter time-to-peak correlates with inflammation, it could be suggested that the visible light susceptible leukocyte reflect a pre-inflammatory state of their donors.

  16. Isoluminol-enhanced chemiluminescence: A sensitive method to study the release of superoxide anion from human neutrophils

    Microsoft Academic Search

    Helen Lundqvist; Claes Dahlgren

    1996-01-01

    A very sensitive chemiluminescence (CL) method was developed for the determination of respiratory burst products generated by the NADPH-oxidase in human neutrophils. Despite the fact that the CL reaction is peroxidase dependent, hydrogen peroxide was found not to participate in the light generating reaction. Phagocytic cells were mixed with isoluminol, a chemiluminescence substrate that detects extracellularly released oxygen species only.

  17. Fast determination of thiacloprid by photoinduced chemiluminescence.

    PubMed

    Catalá-Icardo, Mónica; López-Paz, José Luis; Pérez-Plancha, Laura María

    2014-01-01

    A new and sensitive application of chemiluminescence detection has been developed for the determination of the pesticide thiacloprid in water. It was based on the on-line photoreaction of thiacloprid in a basic medium, with quinine acting as the sensitizer of the chemiluminescent response; cerium (IV) in sulfuric acid medium was used as the oxidant. High degrees of automation and reproducibility were achieved using a flow-injection analysis (FIA) manifold. The validation of the method was performed in terms of selectivity, linearity, limit of detection (LOD), precision, and accuracy. Liquid chromatography with ultraviolet (UV) detection was used as reference for mineral, tap, ground, and spring water samples. The proposed method is fast (with a throughput of 130 h(-1)), sensitive (LOD of 0.8 ng mL(-1) without preconcentration steps and of 0.08 ng mL(-1) with solid-phase extraction [SPE]), low cost, and possible to couple with separation methods for the simultaneous determination of other pesticides. The enhanced chemiluminescence intensity was linear with the thiacloprid concentration above the 2-80 and 80-800 ng mL(-1) ranges. A possible reaction mechanism is also discussed. PMID:25014719

  18. Energy balance and branching ratios for the chemiluminescent Si?NO 2 reaction: Formation of SiO a 3? +, ?' ? 0, and ultrafast a 3? + -b 3? E?E energy transfer

    NASA Astrophysics Data System (ADS)

    Woodward, R.; Hayden, J. S.; Gole, J. L.

    1985-11-01

    Silicon atoms react under single collision conditions with NO 2 to yield intense chemiluminescent emission from the SiO a 3? +-X 1? + and b 3?-X 1? + intercombination band systems and weak emission from the A 1?-X 1? + band system While the cross section for formation of the SiO a 3? + and b 3? states appears to be notably greater than that for the far more exothermic Si + N 2O reaction, the observed intercombination emissions from the Si?NO 2 reaction involve far fewer b 3? vibrational quantum levels and hence considerably less spectral overlap. This facilitates the first observation of emission from SiO a 3? + vibrational quantum levels, ?' 0. Pressure-dependent studies demonstrate that the SiO A 1? and b 3? ( Poxidant < 3 × 10 -4 Torr) states are formed in processes first order in oxidant while some collision-induced population of the very long-lived SiO a 3? + state (?'= 0) may occur in combination with direct product formation even at very low pressures. Temperature-dependent studies are used to indicate that reaction has occurred with ground-state Si 3P atoms and that the SiO b 3? ( EA = 4.0±1.1 kcal/mole) and a 3? + ( EA = 1.8±1.2 kcal/mole) states are formed with a considerably lower activation energy than the A 1? state ( EA = 7.4±1.2 kcal/mole). The activation energy for formation of the a 3? + state, which may be negligible, is significantly smaller than that for formation of the b 3? state, providing some evidence for a dynamical process involving formation of a 3? + at large internuclear distance and possible subsequent transfer to b 3? rovibronic levels to which the a 3? + state is strongly coupled. Higher-pressure studies also provide support for this mechanism. An analysis of the weak emission from the SiO A 1? state allows a determination of a lower bound ( Doo(SiO) ? 186.7 ± 1.7 kcal/mole) for the SiO bond energy. The controlled extension of the outlined single collision studies to elevated pressures (1) leads to a quenched SiO A 1? emission feature, (2) provides additional information on SiO a 3? + vibrational quantum levels, ?' > 0 and (3) indicates, through a comparison of single and multiple collision chemiluminescent spectra, the presence of a significant ultrafast E?E transfer between the SiO a 3? + and b 3? states, the dominant intramolecular process corresponding to SiO a 3? +, ?'= 3 ÷ Ar ? SiO b 3?, ? = 2 + Ar.

  19. Phagocytic and chemiluminescent responses of mouse peritoneal macrophages to living and killed Salmonella typhimurium and other bacteria

    SciTech Connect

    Tomita, T.; Blumenstock, E.; Kanegasaki, S.

    1981-06-01

    In the presence of luminol, resident as well as thioglycolate-induced and immunized macrophages emitted chemiluminescence more efficiently when the cells were exposed to living Salmonella typhimurium than when they were exposed to the same bacterium killed by ultraviolet light or heat. This phenomenon was observed whether or not the bacterium was opsonized. The different response to living and killed bacteria was also found with Escherichia coli, Pseudomonas aeruginosa, Proteus morganii, and Enterobacter aerogenes, but not with Shigella sonnei, Klebsiella pneumoniae, and Propionibacterium acnes. The results suggest that macrophages respond better to living, motile bacteria than to nonmotile or killed bacteria. The experimental results obtained with motility mutants of S. typhimurium, E. coli, and P. aeruginosa confirm that macrophages exposed to the motile bacteria emit chemiluminescence more efficiently and ingest the motile bacteria at a much faster rate than the nonmotile bacteria.

  20. Structure sensitivity in the kinetics and the dynamics of CO oxidation over stepped Pd(335) studied by the molecular beam infrared chemiluminescence technique: Determination of working sites during the steady-state reaction

    SciTech Connect

    Uetsuka, H.; Watanabe, K.; Kimpara, H.; Kunimori, K.

    1999-08-31

    Kinetics and dynamics of CO oxidation have been studied on a stepped Pd(335) surface at a steady-state condition and compared with those on flat Pd(111). The infrared (IR) chemiluminescence technique was applied to determine where the active catalytic sites are on the Pd(335) surface. Since the vibrational energy state of the product CO{sub 2} is sensitive to the structures of the reaction sites on Pd surfaces, information about the working reaction sites during the steady-state CO oxidation can be obtained from the IR emission spectra of the product CO{sub 2}. The production rate of CO{sub 2} was higher on Pd(335) than on Pd(111), indicating that the steps on the surface enhance the catalytic activity for Co oxidation under the steady-state condition. However, the rate data do not necessarily show the real active sites for the CO + O recombination reaction. At a surface temperature of 850 K, the vibrational Boltzmann temperature (T{sub v}) of the product CO{sub 2} on Pd(335) was quite different from (much lower than) that on Pd(111), although the Pd(335) surface has four-atom wide (111) terraces. The lower T{sub v} value on Pd(335) was similar to that on Pd(110)(1 x 1), indicating that a relatively linear activated CO{sub 2} complex was formed. Therefore, during the steady-state CO oxidation on Pd(335), the reaction does not take place on the (111) terrace sites, but mostly on the step sites at 850 K. On the contrary, as the CO coverage increased at a lower surface temperature and at a high CO/O{sub 2} ratio, the T{sub v} values on Pd(335) tend to approach those on Pd(111), indicating that the contribution of the active sites on the steps is decreased and the working reaction sites shift to the (111) terrace sites.

  1. Single-bubble sonochemiluminescence in aqueous luminol solutions.

    PubMed

    Hatanaka, Shin-Ichi; Mitome, Hideto; Yasui, Kyuichi; Hayashi, Shigeo

    2002-09-01

    Sonochemiluminescence (SCL) of luminol due to a single bubble is studied through spectral measurement. No SCL was observed from a stable single bubble that emitted high-intensity sonoluminescence (SL). In contrast, SCL was observed under conditions of an unstable dancing bubble, where a bubble grows and ejects tiny bubbles, making it "dance" by counteraction. Furthermore, SCL was observed from dancing bubbles even when SL was not observed, depending on the dissolved gas content. The instability of bubble collapse is the key parameter governing SCL. PMID:12197706

  2. CHEMILUMINESCENCE MEASUREMENT OF REACTIVITY WEIGHTED ETHYLENE-EQUIVALENT HYDROCARBONS

    EPA Science Inventory

    A reactive hydrocarbon analyzer (RHA), based on the chemiluminescent reaction of hydrocarbons with oxygen atoms, is used to provide a rapid indication of reactivity weighted hydrocarbon mass in automobile exhaust. Samples are reported by their ethylene-equivalent concentration--t...

  3. Demonstration of opsonizing antibodies to Francisella tularensis by leukocyte chemiluminescence.

    PubMed Central

    Löfgren, S; Tärnvik, A; Carlsson, J

    1980-01-01

    Twenty-three individuals were vaccinated with a viable attenuated strain of Francisella tularensis, and blood was collected at various time intervals during 4 weeks. To demonstrate opsonizing antibodies, a mixture of serum and vaccine bacteria was incubated, whereafter the chemiluminescence response of polymorphonuclear (PMN) leukocytes to this mixture was recorded. No opsonizing antibodies against F. tularensis were found in sera obtained before vaccination. Eleven days after vaccination, sera from nine individuals, and 21 days after vaccination, sera from all 23 individuals contained antibodies. Antibodies were demonstrated earlier with the chemiluminescent technique that with the agglutination reaction. Heat treatment (56 degrees C, 30 min) or removal of complement component C3 from immune serum reduced the chemiluminescent response of the leukocytes. A high chemiluminescent response of the leukocytes was induced by immunoglobulin G (IgG)- and IgM-enriched fractions of immune serum in the presence of complement. In the absence of complement, the IgG fraction induced a low chemiluminescent response; the IgM fraction induced no response at all. PMID:7216416

  4. Chemiluminescence and bioluminescence microbe detection

    NASA Technical Reports Server (NTRS)

    Taylor, R. E.; Chappelle, E.; Picciolo, G. L.; Jeffers, E. L.; Thomas, R. R.

    1978-01-01

    Automated biosensors for online use with NASA Water Monitoring System employs bioluminescence and chemiluminescence techniques to rapidly measure microbe contamination of water samples. System eliminates standard laboratory procedures requiring time duration of 24 hours or longer.

  5. Chemiluminescent prediction of service life

    NASA Technical Reports Server (NTRS)

    Hassell, J. A.; Mendenhall, G. D.; Nathan, R. A.

    1976-01-01

    Technique can be used to predict polymer degradation under actual expected-use conditions, without imposing artificial conditions. Smooth or linear correlations are obtained between chemiluminescence and physical properties of purified polymer gums.

  6. Detection of nucleic acid hybrids by prolonged chemiluminescence

    SciTech Connect

    Dattagupta, N.; Clemens, A.H.

    1988-12-27

    A method for determining a particular single stranded polynucleotide sequence in a test medium, comprising the steps of: (a) immobilizing on a solid support single stranded nucleic acids in the test medium, (b) contacting the immobilized nucleic acids with a polynucleotide probe having a base sequence substantially complementary to the sequence to be determined and the contacting being under conditions favorable to hybridization between the probe and the sequence to be determined, wherein the probe is labeled with a chemiluminescence enhancer, (c) separating the immobilized hybrids from the unhybridized probe, (d) initiating a chemiluminescent reaction by contacting the separated, labeled, immobilized hybrids with an oxidant, a 2.3-dihydro-1,4-phthalazinedione chemiluminescence precursor, and a peroxidase enzyme, (e) detecting the resulting light emission, and (f) relating the amount of emitted light to the amount of the single stranded polynucleotide sequence.

  7. A metal (Co)-organic framework-based chemiluminescence system for selective detection of l-cysteine.

    PubMed

    Yang, Na; Song, Hongjie; Wan, Xiangyu; Fan, Xiaoqing; Su, Yingying; Lv, Yi

    2015-03-30

    A metal (Co)-Organic Framework (Co-MOF) was first found to catalyze the chemiluminescence (CL) of luminol. On the basis of X-ray photoelectron spectroscopy, powder X-ray diffraction, CL spectral, UV-visible absorption spectral, and electron spin resonance (ESR) spectral studies, as well as the research of the influence of various free radical scavengers, a possible CL mechanism was proposed. The enhanced CL might be attributed to the formation of a peroxide analogous complex between the oxygen-related radicals and the active metal site of the Co-MOF material. The established Co-MOF-luminol CL system was successfully applied to determine l-cysteine (CySH), based on the selective and sensitive enhancing effect of CySH on this CL system. Under the optimized conditions, CySH was selectively detected in the range 0.1-10 ?M with a detection limit of 18 nM. This novel CL system obviously gives impetus to the new research field of metal-organic frameworks (MOFs) in chemiluminescence. PMID:25697303

  8. An enhanced chemiluminescence resonance energy transfer system based on target recycling G-guadruplexes/hemin DNAzyme catalysis and its application in ultrasensitive detection of DNA.

    PubMed

    Chen, Jia; Huang, Yong; Vdovenko, Marina; Sakharov, Ivan Yu; Su, Guifa; Zhao, Shulin

    2015-06-01

    An enhanced chemiluminescence resonance energy transfer (CRET) system based on target recycling G-guadruplexes/hemin DNAzyme catalysis was developed for ultrasensitive detection of DNA. CRET system consists of luminol as chemiluminescent donor, and fluorescein isothiocyanate (FITC) as acceptor. The sensitive detection was achieved by using the system consisted of G-riched DNA, blocker DNA, and the Nb.BbvCI biocatalyst. Upon addition of target DNA to the system, target DNA hybridizes with the quasi-circular DNA structure, and forms a DNA duplex. The formation of DNA duplex triggers selective enzymatic cleavage of quasi-circular DNA by Nb.BbvCI, resulting in the release of target DNA and two G-riched DNAzyme segments. Released target DNA then hybridizes with another quasi-circular DNA structure to initiate the cleavage of the quasi-circular DNA structure. Eventually, each target DNA can go through many cycles, resulting in the digestion of many quasi-circular DNA structures, generating many G-riched DNAzyme segments. G-riched DNAzyme segment products assemble with hemin to form stable hemin/G-quadruplexes that exhibit peroxidase-like activity which can catalyze the oxidation of luminol by H2O2 to produce CL signals. In the presence of FITC, CL of luminol can excite FITC molecules, and thus produced CRET between the luminol and FITC. This unique analysis strategy gives a detection limit down to 80fM, which is at least four orders of magnitude lower than that of unamplified DNA detection methods. PMID:25863372

  9. Online phototransformation-flow injection chemiluminescence determination of triclosan.

    PubMed

    Song, Shujuan; Song, Qi Jun; Chen, Zhongliang

    2007-04-01

    A highly selective and sensitive chemiluminescence method for the determination of triclosan is proposed. The method is based on the phototransformation of triclosan to a light-emitting precursor in the presence of fluorescein in alkaline medium and the chemiluminescence reaction is then triggered by strong base or oxidants such as N-bromosuccinimide. Based on this reaction an online phototransformation-flow injection manifold was developed, in which the photoreactor comprises a 150-cm-long x 0.8-mm-i.d. piece of PTFE tubing coiled around a 25-W fluorescent lamp, and the phototransformed products were then injected into a carrier stream of borate buffer. After mixing with the oxidant stream the produced light was detected by a photomultiplier. A wide calibration range from 8.0 x 10(-8) to 1.0 x 10(-4) mol L(-1) was obtained under the optimized conditions, and the detection limit was as low as 5.0 x 10(-8) mol L(-1). The whole process of analysis, including the online phototransformation and subsequent chemiluminescence detection, could be completed in 6 min. Most of the foreign substances tested showed high tolerance levels, and the proposed method was directly applied to the determination of triclosan in toothpaste samples without any pre-separation procedure. Figure Schematic representation of the phototransformation of triclosan and subsequent chemiluminescence reaction. PMID:17294174

  10. Mechanism for the chemiluminescence in oxygen-phosphorus systems

    SciTech Connect

    Hamilton, P.A.; Murrells, T.P.

    1986-01-02

    The chemiluminescent reactions of oxygen with a variety of phosphorus-containing compounds have been examined in a discharge-flow/mass spectrometer system. Various qualitative tests and the correlation of the chemiluminescence with the mass spectrometric signals lend strong support to the suggestion that the green emitter is PO/sub 2/, formed in the reaction O + PO ..-->.. PO/sub 2/ + hnu. The results cannot be explained in terms of the PO excimer model. The blue emission seen in the phosphine system is not from the reaction of OH + PO as previously suggested but is tentatively assigned to the reaction O + POH ..-->.. PO/sub 2/H + hnu. 17 references, 3 figures.

  11. Stimulus specific effect of ibuprofen on chemiluminescence of sheep neutrophils

    SciTech Connect

    Tahamont, M.V.; Margiotta, M.; Gee, M.H.

    1986-03-05

    The authors have shown that pretreatment with ibuprofen inhibits free radical release from complement stimulated neutrophils. To further examine the effect of ibuprofen on neutrophil free radical release, they stimulated neutrophils with the synthetic peptide, FMLP, phorbol myristate acetate (PMA), or zymosan-activated plasma (ZAP). Pure (>95%), viable (>95%) sheep neutrophils (2 x 10/sup 6/) were placed in HEPES buffer, luminol, drug or vehicle and stimulated in the luminometer with one of the stimuli. The chemiluminescence (CL) response was recorded and the drug treated samples were compared to vehicle treated controls. Ibuprofen had a dose dependent effect on CL in ZAP stimulated neutrophils. At the highest dose (10/sup -2/M) these cells produced only 37 +/- 7% of the CL response observed in the control cells. In contrast, at the same dose, ibuprofen did not significantly attenuate CL seen in FMLP stimulated cells, with these cells producing 79 +/- 7% of the control cells; nor did ibuprofen effect PMA stimulated CL, as these cells produced a CL response that was 85 +/- 8% of the control cells. Ibuprofen appears to have a stimulus specific effect on free radical release in activated neutrophils. It is also apparent that ibuprofen inhibits complement stimulated free radical release by some mechanism independent of its cyclooxygenase inhibitory effect.

  12. Silver nanoparticle-based chemiluminescent sensor array for pesticide discrimination.

    PubMed

    He, Yi; Xu, Bo; Li, Wenhao; Yu, Haili

    2015-03-25

    In this work, we developed a simple, facile, and highly sensitive nanoparticle-based chemiluminescent (CL) sensor array for the discrimination of organophosphate and carbamate pesticides. This CL sensor array is based on simultaneous utilization of the triple-channel properties of the luminol-functionalized silver nanoparticle (Lum-AgNP) and H2O2 CL system containing CL intensity, the time for CL emissions to appear, and the time to reach the CL peak value, which are able to be measured via a single experiment. The triple-channel properties can be simultaneously altered after interaction with pesticides, producing distinct CL response patterns as "fingerprints" related to each specific pesticide, which was subjected to principal component analysis (PCA) to generate a clustering map. Using this sensor array, five organophosphate and carbamate pesticides, including dimethoate, dipterex, carbaryl, chlorpyrifos, and carbofuran, have been well-distinguished at a concentration of 24 ?g/mL. A total of 20 unknown pesticide samples have been successfully identified with an accuracy of 95%. The simple strategy of this study is expected to promote the development of functionalized nanomaterial-based sensor arrays. PMID:25751408

  13. Theoretical prediction of the photoinduced chemiluminescence of pesticides.

    PubMed

    Ricart, I Sahuquillo; Antón-Fos, G M; Duart, M J; Mateo, J V García; Zamora, L Lahuerta; Calatayud, J Martínez

    2007-04-30

    Although it is relatively easy to find chemiluminescent (CL) molecules working on the field of direct liquid phase (especially employing strong oxidants), the molecules found as chemiluminescent are normally very weak CL compounds for developing suitable analytical CL-procedures. Therefore, it is mandatory to develop new strategies to enhance in a simple way the native chemiluminescence of such a compounds, and even to increase the number of compounds to be determined by direct chemiluminescence. Photoinduced chemiluminescence (Ph-CL) results in a simple and easily on-line accessible strategy to solve these disadvantages. In the present paper, molecular connectivity, a topological method which allows an unique mathematical characterization of molecular structures by the so-named topological descriptors and their correlation with physical, chemical and biological properties of molecules was applied to predict the Ph-CL in liquid phase. Molecular connectivity calculations and discriminant analysis was applied to 72 pesticides for which either a Ph-CL or non Ph-CL behaviour was observed in an experimental screening. The screening test is based on the on-line photodegradation of pesticides by using an automated multicommutation based flow asssembly provided with a photoreactor consisting of 150 cm x 0.8mm PTFE tubing helically coiled around a 20 W low-pressure mercury lamp. Photodegraded pesticides are detected by direct chemiluminescence of the resulting photo-fragments and their subsequent reaction with potassium permanganate in sulfuric acid medium as oxidant. The screening comprised pesticides with different molecular structures and relevant members of the most important families of pesticides were tested (oxime carbamates, sulfonylcarbamates, thiocarbamates, 1,3,5-triazines, organophosphorous, hydroxybenzonitrile, sulfonylureas, phosphonic acid derivatives, imidazolinones, carboxamides, aryloxyalkanoic acids, 1,2,4-triazinones, etc.). The theoretical predictions agree with the empirical results obtained by means of the screening test performed in the multicommutation flow-assembly. PMID:19071628

  14. 3D-printed and CNC milled flow-cells for chemiluminescence detection.

    PubMed

    Spilstead, Kara B; Learey, Jessica J; Doeven, Egan H; Barbante, Gregory J; Mohr, Stephan; Barnett, Neil W; Terry, Jessica M; Hall, Robynne M; Francis, Paul S

    2014-08-01

    Herein we explore modern fabrication techniques for the development of chemiluminescence detection flow-cells with features not attainable using the traditional coiled tubing approach. This includes the first 3D-printed chemiluminescence flow-cells, and a milled flow-cell designed to split the analyte stream into two separate detection zones within the same polymer chip. The flow-cells are compared to conventional detection systems using flow injection analysis (FIA) and high performance liquid chromatography (HPLC), with the fast chemiluminescence reactions of an acidic potassium permanganate reagent with morphine and a series of adrenergic phenolic amines. PMID:24881540

  15. A paper-based chemiluminescence device for the determination of ofloxacin

    NASA Astrophysics Data System (ADS)

    Liu, Wei; Guo, Yumei; Li, Huifang; Zhao, Mei; Lai, Zesheng; Li, Baoxin

    2015-02-01

    Paper-based devices are biodegradable and have been used in diagnosis and environmental analysis field. In this work, a wax-printed paper-based analytical device combined with silver nanoparticles (AgNPs) catalyzed luminol chemiluminescence (CL) system for the determination of ofloxacin (OFLX) was presented. It was based on the enhancement of CL intensity of luminol-H2O2-OFLX system by AgNPs. Wax-printing fabrication technique was used to make the simple circle shaped paper device and large scale chips can be fabricated at the same time. Under the selected experimental conditions, a linear relationship was obtained between the CL intensity and the concentration of OFLX in the range from 1.0 × 10-9 g/mL to 1.0 × 10-6 g/mL with a detection limit of 3.0 × 10-10 g/mL. This method has been successfully applied to the determination of OFLX in eyedrop samples.

  16. Revealing interaction between sulfobutylether-?-cyclodextrin and reserpine by chemiluminescence and site-directed molecular docking.

    PubMed

    Xiong, Xunyu; Wu, Min; Zhao, Xinfeng; Song, Zhenghua

    2014-09-01

    The host-guest interaction between sulfobutylether-?-cyclodextrin (SBE-?-CD) and reserpine (RSP) is described using flow injection-chemiluminescence (FI-CL) and site-directed molecular docking methods. It was found that RSP could inhibit the CL intensity produced by a luminol/SBE-?-CD system. The decrease in CL intensity was logarithmic over an RSP concentration range of 0.03 to 700.0 nM, giving a regression equation of ?I?=?107.1lgCRES ?+?186.1 with a detection limit of 10 pM (3?). The CL assay was successfully applied in the determination of RSP in injection, saliva and urine samples with recoveries in the range 93.5-106.1%. Using the proposed CL model, the binding constant (KCD-R ) and the stoichiometric ratio of SBE-?-CD/RSP were calculated to be 7.4?×?10(6) ?M(-1) and 1 : 1, respectively. Using molecular docking, it was confirmed that luminol binds to the small cavity of SBE-?-CD with a nonpolar interaction, while RSP targeted the larger cavity of SBE-?-CD and formed a 1 : 1 complex with hydrogen bonds. The proposed new CL method has the potential to become a powerful tool for revealing the host-guest interaction between CDs and drugs, as well as monitoring drugs with high sensitivity. PMID:24127401

  17. Supramolecular assembly and nanostructures of a series of luminol derivatives with aromatic/alkyl substituted groups in Langmuir-Blodgett films.

    PubMed

    Jiao, Tifeng; Xing, Yuanyuan; Zhang, Qingrui; Zhang, Li; Liu, Minghua; Zhou, Jingxin; Gao, Faming

    2014-06-01

    A series of functional luminol derivatives with aromatic and alkyl substituted groups has been designed and synthesized from the reaction of the corresponding chloride precursors with luminol. These compounds can be spread on water surface to form stable Langmuir films at the air-water interface. It has been found that UV and IR spectra confirmed the characteristic aromatic segment, imide group, and aromatic/alkyl substituted groups. In addition, for the interfacial assembly process of compounds with alkyl substituted groups, there are obvious spectral changes for the alkyl chains. AFM results indicated that various different aggregated domains may be fabricated in the transferred LB films. For all cases, the substituted groups in molecular structures have an important effect in regulating the aggregation mode and spectral changes in organized molecular films. The present results showed that the modified luminol derivatives may have potential application in functional material fields such as ECL sensor, which may give some insight to study the relationship between the molecular structures and supramolecular aggregation of amphiphiles in organized molecular films. PMID:24738403

  18. The Kinetics and Dynamics of AN Atmospheric Reaction System Using Chemiluminescence, Laser-Induced Fluorescence and Stimulated Raman Excitation: Hydrogen Sulfide + Nitrous Oxide

    Microsoft Academic Search

    K. Ravichandran

    1995-01-01

    Acid rain and the greenhouse effect are two of the most important environmental issues of this century. Oxidation of reduced sulfur compounds is responsible for acid rain, and the increase in concentration and subsequent reactivity of vibrationally excited greenhouse gases are important to understand global warming. The following reaction system addresses these two issues and forms the basis for the

  19. Determination of sibutramine with a new sensor based on luminol electrochemiluminescence

    Microsoft Academic Search

    Shaowei Wang; Jinghua Yu; Fuwei Wan; Shenguang Ge; Mei Yan; Meng Zhang

    2011-01-01

    A flow injection electrochemiluminescence (FI-ECL) analysis method for the determination of sibutramine in the presence of luminol was studied under conventional cyclic voltammetry in alkaline Na2CO3–NaHCO3 buffer solution (pH 8.0–12.0). This method is based on the enhanced ECL of luminol–sibutramine. Meanwhile, in order to overcome the drawbacks of conventional cells, a FI cell was designed, which is reusable and has

  20. Hydroxyl radical-induced characteristic chemiluminescent spectra from plasma of hemodialysis patients.

    PubMed

    Agatsuma, S; Nagoshi, T; Kobayashi, M; Usa, M; Watanabe, H; Sekino, H; Inaba, H

    1992-01-01

    Plasma from hemodialysis patients evoked weak photon emissions (chemiluminescence) in a characteristic emission spectrum with a peak at 430 nm, attributed to attack by hydroxyl radicals generated from the iron-catalyzed breakdown of hydrogen peroxide (Fenton reaction), whereas plasma from normal healthy subjects showed a rather weak red chemiluminescence peak at around 680 nm, similar to that resulting from attack by hydroxyl radicals. However, the addition of hydrogen peroxide in the absence of divalent irons induced almost the same red chemiluminescent emission spectrum in both plasmas. The HPLC-gel-filtration chromatography carried out with both plasmas revealed that a primary emitter evoking a peak emission at 430 nm was located in the fraction of lower-molecular-mass substances in fractionated plasma from hemodialysis patients. In contrast, the elution peaks evoking red chemiluminescence with the addition of hydrogen peroxide were mainly observed for the higher-molecular-mass fraction, as determined by gel chromatography of both plasmas. Therefore, the observation of a chemiluminescence peak at 430 nm, induced by the generation of hydroxyl radicals, correlated well with chemiluminescent emissions in plasma samples from patients with chronic renal failure. Spectral analyses of clinical samples that show weak chemiluminescence by forced oxidation by such an active oxygen may provide a new and more sensitive method for diagnosing metabolic disorders. PMID:1310266

  1. Chemiluminescence Study on Thermal Degradation of Aircraft Tire Elastomers

    NASA Technical Reports Server (NTRS)

    Mendenhall, G. D.; Stanford, T. B.; Nathan, R. A.

    1976-01-01

    Since the autoxidative process accounts in part for the degradation of rubber, including aircraft tires, it was felt that a study of the chemiluminescence from unsaturated elastomers could contribute significantly to an understanding of the degradation mechanism. The study revealed similarities in chemiluminescence behavior between four elastomers which were investigated, and it shows that similar oxidation mechanisms occur. Oxidative chemiluminescence was observed from purified samples of cis-1,4-polybutadiene, cis-1,4-polyisoprene, trans-polypentenamer, and 1,2-polybutadiene in an oxygen atmosphere at 25-150 C. The elastomer samples were placed in a 600 watt oven which is equipped with gas inlets for introducing any desired atmosphere. Chemiluminescence emission from the samples was focused with a two inch quartz lens onto the detector of a 12" photomultiplier which is connected to a photon counter. A strip-chart recorder, connected to the counter, permitted automatic data collection. Diagrams of the apparatus are included. The chemical reactions which occurred from the thermal decomposition of the polymer samples are described, and results (and tabulated data) are discussed.

  2. CHEMILUMINESCENT MONITOR FOR VINYL CHLORIDE

    EPA Science Inventory

    A monitor for vinyl chloride monomer (VCM) in ambient air was constructed using commercially available components of a gas chromatograph (GC) coupled with a chemiluminescence ozone analyzer slightly modified to make it suitable for use as a GC detector. The specificity for VCM is...

  3. Simplified ozone detection by chemiluminescence

    NASA Technical Reports Server (NTRS)

    Conway, E. J.; Rogowski, R. S.; Richards, R. R.

    1977-01-01

    Ozone is detected by film coated with solid, such as rubrene, that reacts with ozone to degree proportional to concentration in sample gas. Gas flow is stopped, and film is heated to produce light (chemiluminescence) in proportion to amount of reacted material on sensor.

  4. NO A{sup 2}{Sigma}{sup +}-X{sup 2}II chemiluminescence produced from the reaction of excited NO{sub 2} with acetylene and its derivatives in their triplet states

    SciTech Connect

    Sisk, Wade; Endo; Hiromu; Shibuya, Kazuhiko; Obi, Kinichi [Tokyo Institute of Technology, Meguro, Tokyo (Japan)

    1992-08-06

    This paper discusses how reacting excited NO{sub 2} with acetylene and its derivatives produced NO(A{sup 2}{Sigma}{sup +}) by a NO A{sup 2}{sigma}{sup +}-X{sup 2}II chemiluminescence produced by a triplet-state mechanism, with acetylene > methylacetylene > ethylacetylene > phenylacetylene > benzene in terms of efficiency. 22 refs., 8 figs., 2 tabs.

  5. Enhanced chemiluminescent detection scheme for trace vapor sensing in pneumatically-tuned hollow core photonic bandgap fibers

    E-print Network

    Stolyarov, Alexander Mark

    We demonstrate an in-fiber gas phase chemical detection architecture in which a chemiluminescent (CL) reaction is spatially and spectrally matched to the core modes of hollow photonic bandgap (PBG) fibers in order to enhance ...

  6. A magnetic particles-based chemiluminescence enzyme immunoassay for rapid detection of ovalbumin.

    PubMed

    Feng, Xiao-Li; Ren, Hong-Lin; Li, Yan-Song; Hu, Pan; Zhou, Yu; Liu, Zeng-Shan; Yan, Dong-Ming; Hui, Qi; Liu, Dong; Lin, Chao; Liu, Nan-Nan; Liu, Yan-Yan; Lu, Shi-Ying

    2014-08-15

    Egg allergy is an important public health and safety concern, so quantification and administration of food or vaccines containing ovalbumin (OVA) are urgently needed. This study aimed to establish a rapid and sensitive magnetic particles-chemiluminescence enzyme immunoassay (MPs-CLEIA) for the determination of OVA. The proposed method was developed on the basis of a double antibodies sandwich immunoreaction and luminol-H2O2 chemiluminescence system. The MPs served as both the solid phase and separator, the anti-OVA MPs-coated polyclonal antibodies (pAbs) were used as capturing antibody, and the horseradish peroxidase (HRP)-labeled monoclonal antibody (mAb) was taken as detecting antibody. The parameters of the method were evaluated and optimized. The established MPs-CLEIA method had a linear range from 0.31 to 100ng/ml with a detection limit of 0.24ng/ml. The assays showed low reactivities and less than 5% of intraassay and interassay coefficients of variation (CVs), and the average recoveries were between 92 and 97%. Furthermore, the developed method was applied in real samples analysis successfully, and the correlation coefficient with the commercially available OVA kit was 0.9976. Moreover, it was more rapid and sensitive compared with the other methods for testing OVA. PMID:24769049

  7. Novel FIA chemiluminescence fiber optic biosensor for urinary and blood glucose

    NASA Astrophysics Data System (ADS)

    Cattaneo, Maurice V.; Luong, J. H. T.

    1993-05-01

    A chemiluminescence fiber optic biosensor system coupled to FIA was developed to measure glucose in bodily fluids. Glucose oxidase was immobilized on a preactivated nylon membrane and attached to the tip of a fiber optic bundle. This enzyme acts on (beta) -D-glucose to produce hydrogen peroxide which was then reacted with luminol in the presence of ferricyanide to produce a light signal. The sensitivity of the biosensor was determined to be 32 +/- 0.65 nV (mu) M-1 with a minimum detectable level of 5 (mu) M. The addition of a glucose oxidase column with a higher enzyme loading improved the sensitivity by at least 25-fold thus permitting the measurement of the lower glucose levels found in urine. The enzyme membrane could be reused for at least 50 analyses while the glucose oxidase column could be reused for over 500 analyses without losing the original activity. Endogenous ascorbate and urate usually present in urine samples which interfere with the chemiluminescence signal were effectively retained by an upstream ion exchange column. When applied for the determination of urinary and blood glucose levels, the results obtained compared well with those of the widely accepted hexokinase assay.

  8. Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.

    PubMed

    Vdovenko, Marina M; Stepanova, Alexandra S; Eremin, Sergei A; Van Cuong, Nguyen; Uskova, Natalia A; Yu Sakharov, Ivan

    2013-11-15

    Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104?g/kg) was significantly higher than that in mandarins (1.66-2.82?g/kg). PMID:23790860

  9. A comparison of flame ionization and ozone chemiluminescence for the determination of atmospheric hydrocarbons.

    SciTech Connect

    Marley, N. A.; Gaffney, J. S.; Environmental Research

    1998-01-01

    A reactive hydrocarbon analyzer has been constructed on the basis of chemiluminescence reaction with ozone. This detector is designed to operate at varying temperatures which take advantage of the different rates of reaction of the hydrocarbon classes with ozone to yield a measure of their atmospheric reactivity. When operated at high temperatures (170 C), all hydrocarbons will give a chemiluminescence signal. Reported here is a direct comparison of the ozone chemiluminescent detector (operated at a temperature of 170 C) with a flame ionization detector. This comparison was accomplished by connecting a capillary gas chromatograph to each of the two detectors by means of a switching valve. Twenty-seven compounds representing alkanes, alkenes, aromatics, and oxygenated hydrocarbons (aldehydes, ketones, alcohols, and ethers) were studied. For the compounds studied, analytical sensitivities were 10-1000 times better for the chemiluminescence detector. The results of this comparison indicate that the response of the chemiluminescent detector at 170 C correlates with a total carbon detector (flame ionization detection) and that total response is a measure of total carbon in the sample. The chemiluminescent system will be very useful for gas chromatographic detection of atmospheric hydrocarbons, particularly of oxygenates in complex mixtures.

  10. Investigation of singlet oxygen generation in Vit C-Cu2+ -LDL system by chemiluminescence method

    NASA Astrophysics Data System (ADS)

    Wang, Juan; Xing, Da; Tan, Shici; Tang, Yonghong; He, Yonghong

    2002-04-01

    In this study, by chemiluminescence method using a Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLA), as a selective and sensitive chemiluminescence probe, singlet oxygen (1O2) formation was observed in the vit C- LDL-Cu2+ reaction system. Another experimental evidence for the generation of 1O2 was the quenching effect of sodium azide (NaN3) on vit C-induced chemiluminescence in the reaction mixture of LDL- Cu2+-MCLA. Analysis based on the experimental results indicated the plausible reaction mechanism is that vit C converts Cu2+ to its reduced state and vit C becomes vit C radical itself, thereby stimulating the formation of peroxyl radicals, and bimolecular reaction of peroxyl radicals results in 1O2 production in the above systems.

  11. Chemiluminescence from poly(styrene- b-ethylene- co-butylene- b-styrene) (SEBS) block copolymers

    Microsoft Academic Search

    Carmen Peinado; Teresa Corrales; M Jesús García-Casas; Fernando Catalina; Valentín Ruiz Santa Quiteria; M Dolores Parellada

    2006-01-01

    A detailed analysis of the chemiluminescence emission (CL) from poly(styrene-b-ethylene-co-butylene-b-styrene), SEBS, was carried out. A phenol–phosphite stabilization system based on Irgafos 168 and Irganox 1330, was studied. The kinetic analysis of the CL profile under nitrogen shows a first-order reaction for the decay of chemiluminescence. The activation energy shows different values as a function of temperature, showing that different reactions

  12. Precision milled flow-cells for chemiluminescence detection.

    PubMed

    Mohr, Stephan; Terry, Jessica M; Adcock, Jacqui L; Fielden, Peter R; Goddard, Nick J; Barnett, Neil W; Wolcott, Duane K; Francis, Paul S

    2009-11-01

    Novel flow-cells with integrated confluence points and reaction channels designed for efficient mixing of fast chemiluminescence systems were constructed by machining opposing sides of a polymer chip and sealing the channels with transparent epoxy-acetate films. A hole drilled through the chip provided the conduit from the confluence point on one side to the centre of the reaction zone on the other side, allowing rapid presentation of the reacting mixture to the photodetector. The effectiveness of each flow-cell was evaluated by comparing the chemiluminescence intensity using flow injection analysis methodology, and examining the distribution of light emanating from the reaction zone (captured by photography in a dark room) when the reactants were continuously merged. Although previously reported chemiluminescence detectors constructed by machining channels into polymers have almost exclusively been prepared using transparent materials, we obtained far greater emission intensities using an opaque white chip with a thin transparent seal, which minimised the loss of light through surfaces not exposed to the photomultiplier tube. Furthermore, this approach enabled the exploration of reactor designs that could not be incorporated in traditional coiled-tubing flow-cells. PMID:19838409

  13. Multiplex chemiluminescent immunoassay for screening of mycotoxins using photonic crystal microsphere suspension array.

    PubMed

    Xu, Kun; Sun, Yue; Li, Wei; Xu, Jie; Cao, Bin; Jiang, Yunkun; Zheng, Tiesong; Li, Jianlin; Pan, Daodong

    2014-02-21

    A novel multiplex chemiluminescent mycotoxin immunoassay suspension array system was developed by combining the silica photonic crystal microspheres (SPCMs) encoding technique and a chemiluminescent immunoassay (CLIA) method. The SPCMs were used as a carrier of the suspension array and encoded by their reflectance peak positions, which overcome fluorescence photobleaching, and the potential interference between the encoding fluorescence and detection fluorescence. Aflatoxin B1 (AFB1), fumonisin B1 (FB1) and ochratoxin A (OTA) artificial antigens were immobilized on the surfaces of SPCMs by using 3-glycidoxypropyltrimethoxysilane as a linker. Horseradish peroxidase (HRP) was used as a labeling enzyme for the secondary antibody in the enzyme-catalyze H2O2-luminol chemiluminescence system. The CLIA detection system was easily integrated with a multifunctional microplate reader and displayed a two to three orders of magnitude dynamic linear detection range from 0.001 to 1, 0.001 to 1, and 0.01 to 1 ng mL(-1) for AFB1, FB1 and OTA with 50% inhibitory concentrations (IC50) of 0.01, 0.036, and 0.04 ng mL(-1), respectively. The recovery rates are in the range of 63.5 to 121.6% for the three mycotoxins in three kinds of spiked cereal samples. The results of detection in 12 naturally contaminated cereal samples were consistent with that of the classic enzyme-linked immunosorbent assay (ELISA) method. This proposed system is simple, rapid, low cost and high throughput for multiplex mycotoxin assay. PMID:24352570

  14. Chemiluminescent Oscillating Demonstrations: The Chemical Buoy, the Lighting Wave, and the Ghostly Cylinder

    ERIC Educational Resources Information Center

    Prypsztejn, Hernan E.; Mulford, Douglas R.; Stratton, Doug

    2005-01-01

    Oscillating reactions have been extensively used in chemical demonstrations. They involve several chemical concepts about kinetics, catalysts, and thermodynamics. The spontaneous cyclic color change of a solution is an attraction in any educational-level course. Chemiluminescent reactions are also among the most fascinating demonstrations and have…

  15. A firefly inspired one-pot chemiluminescence system using n-propylphosphonic anhydride (T3P).

    PubMed

    Kato, Dai-ichiro; Shirakawa, Daiki; Polz, Robin; Maenaka, Mika; Takeo, Masahiro; Negoro, Seiji; Niwa, Kazuki

    2014-12-01

    A simple reaction procedure for chemiluminescence of firefly luciferin (D-luc) using n-propylphosphonic anhydride (T3P) is reported. A luminescent photon is produced as a result of one-pot reaction, only requiring mixing with the substrate carboxylic acid and T3P in the presence of a mild organic base. PMID:25350893

  16. Solution mixing and the emission of light in flow-cells for chemiluminescence detection.

    PubMed

    Terry, Jessica M; Zammit, Elizabeth M; Slezak, Teo; Barnett, Neil W; Olson, Don C; Wolcott, Duane K; Edwards, Donna L; Francis, Paul S

    2011-03-01

    Constructing flow-through reactors for chemiluminescence detection by machining channels into polymer disks has enabled the exploration of new configurations and materials that can improve signal intensity beyond that attainable with the traditional coiled-tubing design. Several approaches to merge reactant solutions were examined: an intersection, chamber or deeper well in the centre of a serpentine configuration flow-cell (directly in front of a photomultiplier tube), or a confluence point outside the detection zone. For several analytically useful, rapid chemiluminescence reactions, the single-inlet flow-cell with external Y-piece was most suitable, but for others (such as KMnO(4)/Mn(II) with morphine, and [Ir(f-ppy)(2)BPS](-) with fluoroquinolones) the dual-inlet configuration provided greater signals. The introduction of central mixing zones with larger widths than the channel reduced the chemiluminescence response. The reversing turns of a serpentine channel promote efficient mixing and greater chemiluminescence intensities than a spiral channel, but increasing the sharpness of the turns created areas of poor solution flow and decreased the chemiluminescence response. Teflon disks impregnated with glass microspheres increased the chemiluminescence signals by 13%-17%, due to the greater reflection of stray light towards the photodetector. PMID:21127794

  17. A luminol electrochemiluminescence aptasensor based on glucose oxidase modified gold nanoparticles for measurement of platelet-derived growth factor BB.

    PubMed

    Zhang, Jing-Jing; Cao, Jun-Tao; Shi, Gui-Fang; Huang, Ke-Jing; Liu, Yan-Ming; Ren, Shu-Wei

    2015-01-01

    A sandwich-type luminol electrochemiluminescence (ECL) aptasensor for highly sensitive and selective detection of platelet-derived growth factor BB (PDGF-BB) is fabricated. For this proposed ECL aptasensor, a multilayered AuNPs-electrochemically reduced graphene (AuNPs-EG) nanocomposite film was formed on the GCE surface as the base of the aptasensor through a co-electrodeposition method. The AuNPs-EG composites possess high conductivity to promote the electron transfer at the electrode interface and good biocompatibility and large surface area to capture large amounts of primary aptamer (Apt1), thus amplifying the detection response. Moreover, glucose oxidase (GOD) functionalized AuNPs labeled secondary aptamer (GOD-Apt2-AuNPs) was designed as the signal probe for the sandwiched aptasensor. Enhanced sensitivity was obtained by in situ generation of H2O2 from reaction between GOD and glucose and the excellent catalytic behavior of AuNPs to the ECL of the luminol-H2O2 system. Under the optimal conditions, the as-prepared ECL aptasensor exhibited excellent analytical property for the detection of PDGF-BB in the range from 1.0×10(-13) to 5.0×10(-10) mol L(-1) with a detection limit of 1.7×10(-14) mol L(-1) (S/N=3). The application of the present protocol was demonstrated by analyzing PDGF-BB in human serum and human urine samples with the recoveries from 85.0% to 110%. PMID:25476280

  18. Determination of sulfide with acidic permanganate chemiluminescence for development of deep-sea in-situ analyzers.

    PubMed

    Fujimori, Keiichi; Tsujimoto, Kenta; Moriuchi-Kawakami, Takayo; Shibutani, Yasuhiko; Ueda, Masato; Suzue, Takahiko; Kimoto, Hideshi; Okamura, Kei

    2011-01-01

    A new chemiluminescence method is proposed for the determination of sulfide in seawater based on the chemiluminescence reaction between sulfide and an acidic permanganate solution. 3-Cyclohexylaminopropanesulfonic acid was used as a chemiluminescence enhancer. By use of this method, 1-150 µM of sulfide could be determined in artificial seawater. The limit of detection was 0.17 µM sulfide. We investigated the effects of salinity, water temperature, and interfering chemicals such as heavy-metal ions and organic matter. In addition, natural seawater spiked with sulfide was analyzed. The results showed that the CL method could be applied to a deep-sea sulfide analyzer. PMID:21321442

  19. Light Stick Chemistry: Bioluminescence and Chemiluminescence (title provided or enhanced by cataloger)

    NSDL National Science Digital Library

    Michael DiSpezio

    This activity will offer students an introduction to chemiluminescence and bioluminescence, an opportunity to observe the effect of temperature on reaction, and an activity that integrates art and science. Students explore how the temperature of the chemicals that combine affects a chemiluminescent reaction. They will also learn that since solar radiation does not reach the ocean depths, the bottom of the deep ocean remains largely in complete darkness. However, certain animals in this extreme environment can generate bioluminescent light to communicate with each other and even to lure prey.

  20. Solid surface enhancement effects on chemiluminescence

    Microsoft Academic Search

    Tetsutaro Yoshinaga; Yasuo Tanaka; Teijiro Ichimura; Hiroshi Hiratsuka; Michio Kobayashi; Toshihiko Hoshi

    1998-01-01

    Solid surface enhancement effects on chemiluminescence were studied using several natural and synthetic polymers. The influence of surface conditions of cellulose on the chemiluminescence of powder and filter paper was also examined. Intensities for powder were relatively low compared to paper. The influence of packed powder and transparent film with the same particle size was examined for PVA, PVC and

  1. Stress chemiluminescence: predictive applications to polymer failure

    SciTech Connect

    Richardson, J.H.; Monaco, S.B.; Breshears, J.D.; Johnson, D.C.; Lanning, S.M.; Morgan, R.J.

    1981-10-01

    A computer-controlled stress chemiluminescence instrument has been designed and assembled. A LSI-11 microprocessor is used to ramp the stepping motor which applies the stress to the polymer sample. The computer also acquires data from the load cell and the photon counter, and outputs the essential data to either a printer or floppy disk for storage and subsequent manipulation. The stress chemiluminescence technique has been previously reported by Levy and Fanter: the significant result of this work is the correlation of an enhanced chemiluminescence signal in a low stress environment with the subsequent premature mechanical failure of the polymer sample. Currently only epoxy and nylon samples have been examined; not all epoxy polymers appear to have this correlation between mechanical failure and stress chemiluminescence signal (of course, previous work by Levy and Fanter indicated that not all polymers exhibit a correlation between stress and chemiluminescence). The technique will be extended to fibers, composites, and polymers subjected to accelerated aging.

  2. Predicted NO/sub 2/ ir chemiluminescence in the natural atmosphere. Technical report, February-May 1986

    SciTech Connect

    Adler-Golden, S.

    1986-05-13

    Results from the author's recent theoretical study of NO/sub 2/ ir chemiluminescence from the NO + 0 and NO + O/sub 3/ reactions are employed in a new upper atmospheric NO/sub 2/ ir radiance model. Predictions are made for altitude-dependent and column-integrated NO/sub 2/ chemiluminescent radiance in four vibrational bands, and comparison is made with previous model predictions.

  3. Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection

    Microsoft Academic Search

    Jason W. Costin; Simon W. Lewis; Stuart D. Purcell; Lucy R. Waddell; Paul S. Francis; Neil W. Barnett

    2007-01-01

    We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2?-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant

  4. Time course of superoxide generation by leukocytes--the MCLA chemiluminescence system.

    PubMed

    Prónai, L; Nakazawa, H; Ichimori, K; Saigusa, Y; Ohkubo, T; Hiramatsu, K; Arimori, S; Fehér, J

    1992-10-01

    This study was performed to examine the pattern of superoxide (O2-.) generation from leukocytes using the O2-. specific chemiluminescence (CL) method. Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo [1,2-alpha]pyrazin-3-one (MCLA) was used as a CL probe. The appropriate conditions of the MCLA method was first determined for the evaluation of the time course of O2-. generation by leukocytes. The time course of O2-. generation obtained by the MCLA-CL system was compared with that by the luminol-dependent CL, electron spin resonance (ESR)/spin trapping, and cytochrome c systems. Following stimulation by three different stimulants (PMA, OZ, FMLP), leukocytes continuously generated O2-. for up to 5 h in the MCLA-CL system, irrespective of the kind of stimulation. The curves obtained by generation ceased more rapidly in the luminol-CL, ESR/spin trapping, and cytochrome c systems. A 50% activity of the initial value was observed at 70 min in the MCLA-CL system, but 30, 10 and 35 min in the other systems, respectively. The CL or O2-. generation value decreased to less than 1% (possible termination) at 300, 90, 120 and 180 min, respectively. With the exception of ESR studies with OZ, the cell viability was not significantly affected in any of the trials. These results indicate that leukocytes can generate O2-. much longer than previously estimated and that the MCLA-CL-system is the most suitable system for the measurement of the O2-. generation by leukocytes. PMID:1330925

  5. Evaluation of antioxidant activity and preventing DNA damage effect of pomegranate extracts by chemiluminescence method.

    PubMed

    Guo, Shanshan; Deng, Qianchun; Xiao, Junsong; Xie, Bijun; Sun, Zhida

    2007-04-18

    The antioxidant activities of three parts (peel, juice, and seed) and extracts of three pomegranate varieties in China were investigated by using a chemiluminescence (CL) method in vitro. The scavenging ability of pomegranate extracts (PEs) on superoxide anion, hydroxide radical, and hydrogen peroxide was determined by the pyrogallol-luminol system, the CuSO4-Phen-Vc-H2O2 system, and the luminol-H2O2 system, respectively. DNA damage preventing the effect of PE was determined by the CuSO4-Phen-Vc-H2O2-DNA CL system. The results showed that the peel extract of red pomegranate had the best effect on the scavenging ability of superoxide anion because its IC50 value (4.01 +/- 0.09 microg/mL) was the lowest in all PEs. The seed extract of white pomegranate could scavenge hydroxide radical most effectively of the nine extracts (the IC50 value was 1.69 +/- 0.03 microg/mL). The peel extract of white pomegranate had the best scavenging ability on hydrogen peroxide, which had the lowest IC50 value (0.032 +/- 0.003 microg/mL) in the nine extracts. The seed extract of white pomegranate (the IC50 value was 3.67 +/- 0.03 microg/mL) was the most powerful on the DNA damage-preventing effect in all of the PEs. Also, the statistical analysis indicated that there were significant differences (at P < 0.05) among the extracts of the different varieties and parts in each system. PMID:17381116

  6. Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

    PubMed Central

    Novikov, Kirill N.; Berdnikova, Nadezhda G.; Novikov, Alexey K.; Lyusina, Oksana Y.; Muhitova, Olga G.; Yablonskaya, Olga I.; Minh, Ha Do; Voeikov, Vladimir L.

    2012-01-01

    Summary Background Chronic obstructive pulmonary disease (COPD) is an inflammatory disease associated with reactive oxygen species (ROS) production. The aim of this study was to evaluate the effect of Hypoxen® treatment and the effect of HyFnC60 on ROS production in patients’ blood. Material/Methods ROS production in blood was estimated using chemiluminescence (CL) measurement with CL-amplifiers: luminol (LM), LM + zymosan (ZM) or lucigenin (LC) in the presence or absence of hydrated fullerenes (HyFnC60) added to blood in low concentrations. Results In all the patients with COPD in remission phase with Hypoxen® prescription, the LM-dependent CL (LM-CL) with ZM and LC-enhanced CL (LC-CL) decreased after the treatment. Parameters of CL and effects of HyFnC60 upon them depended on blood state. Addition of HyFnC60 to blood decreased data scattering and helped to improve discrimination between different groups of patients. Using the discriminator analysis, we found the most important time-points in the kinetic curves of CL for classification of patients into groups (eg, COPD patients before and after treatment with Hypoxen®; patients’ blood with different sensitivity to HyFnC60 concentration). Conclusions Monitoring of CL of non-diluted whole blood in COPD patients can be used for the estimation of the Hypoxen® efficiency in complex therapy. Addition of HyFnC60 to blood increases sensitivity of the method. PMID:22293870

  7. The reaction of C2H with H2 : Absolute rate coefficient measurements and ab initio study

    E-print Network

    Nguyen, Minh Tho

    Received 15 August 2001; accepted 28 November 2001 In this work, a pulsed laser photolysis/chemiluminescence X 2 ) chemiluminescence resulting from their reaction with O2 . Over the experimental temperature

  8. Chemiluminescence chemical detection of vapors and device

    SciTech Connect

    Collins, G.E.; Rose-Pehrsson, S.L.

    1995-07-10

    A solid phase chemical sensor includes a polymer film which has a chemiluminescent reagent immobilized therein. The polymer film and chemiluminescent reagent are chosen to significantly enhance the selectivity of the sensor to the analyte in the gaseous phase to which the sensor is exposed. The sensor is then positioned so that, when exposed to the gaseous mixture, any chemiluminescence generated will be detected by a photomultiplier tube or other photoelectric device, such as a photodiode. The sensor is particularly useful in the detection of O2, N2H4, SO2, NO2, and halogenated hydrocarbons.

  9. Single and multiple collision chemiluminescent studies of the Si?OCS and Ge?OCS reaction. A study of the SiS and GeS a 3? +—X 1? + and SiS b 3? — X 1? + intercombination systems and the nature of SiS* collisional quenching

    NASA Astrophysics Data System (ADS)

    Green, Gary J.; Gole, James L.

    1980-02-01

    The chemiluminescent emission from silicon and germanium monosulfide resulting from the reaction of silicon and germanium metal with carbonyl sulfide has been studied under both single and multiple collision conditions. Included in this study is the observation of the SiS and GeS a a 3? + - X 1? + and SiS b 3? - X 1? + intercombination band systems. The temperature dependence of the observed single collision spectra is analyzed to deduce the nature of the metal reactant beams and to determine the activation energies for formation of SiS* a 3? +, SiS* b 3? and GeS* a 3? +. The significance of the activation energies is discussed. Lower bounds of 143.7 ± 1.6 kcal/mole and 132.2 ± 1.0 kcal/mole are determined for Duo(SiS) and Doo(GeS), respectively. Multiple collision spectral studies are used to initiate the exploration of collisional quenching.

  10. Chemiluminescent Detection of Oxidants in Vascular Tissue Lucigenin But Not Coelenterazine Enhances Superoxide Formation

    Microsoft Academic Search

    Margaret M. Tarpey; C. Roger White; Edward Suarez; Gloria Richardson; Rafael Radi; Bruce A. Freeman

    Lucigenin-amplified chemiluminescence has frequently been used to assess the formation of superoxide in vascular tissues. However, the ability of lucigenin to undergo redox cycling in purified enzyme-substrate mixtures has raised questions concerning the use of lucigenin as an appropriate probe for the measurement of superoxide production. Addition of lucigenin to reaction mixtures of xanthine oxidase plus NADH resulted in increased

  11. A simple and compact smartphone accessory for quantitative chemiluminescence-based lateral flow immunoassay for salivary cortisol detection.

    PubMed

    Zangheri, Martina; Cevenini, Luca; Anfossi, Laura; Baggiani, Claudio; Simoni, Patrizia; Di Nardo, Fabio; Roda, Aldo

    2015-02-15

    We have developed a simple and accurate biosensor based on a chemiluminescent (CL)-lateral flow immunoassay (LFIA) method integrated in a smartphone to quantitatively detect salivary cortisol. The biosensor is based on a direct competitive immunoassay using peroxidase-cortisol conjugate, detected by adding the chemiluminescent substrate luminol/enhancer/hydrogen peroxide. The smartphone camera is used as light detector, for image acquisition and data handling via a specific application. We 3D-printed simple accessories to adapt the smartphone. The system comprises a cartridge, which houses the LFIA strip, and a smartphone adaptor with a plano-convex lens and a cartridge-insertion slot. This provides a mini-darkbox and aligned optical interface between the camera and the LFIA membrane for acquiring CL signals. The method is simple and fast, with a detection limit of 0.3 ng/mL. It provides quantitative analysis in the range of 0.3-60 ng/mL, which is adequate for detecting salivary cortisol in the clinically accepted range. It could thus find application in the growing area of home-self-diagnostic device technology for clinical biomarker monitoring, overcoming the current difficulties in achieving sensitive and quantitative information with conventional systems taking the advantage of smartphone connectivity and the enhanced performance of the included camera. PMID:25194797

  12. Sorption of platinum on immobilized microorganisms for its on-line preconcentration and chemiluminescent determination in water samples.

    PubMed

    Malejko, Julita; Szyga?owicz, Marzena; Godlewska-?y?kiewicz, Beata; Koj?o, Anatol

    2012-02-01

    Fungi of the type Aspergillus sp. were immobilized on a cellulosic resin and used as a biosorbent for the on-line preconcentration and separation of Pt(IV) ions prior to their chemiluminescent determination via flow injection analysis. Biosorption and elution conditions were optimized, and the results compared to biosorbents based on the use of Chlorella vulgaris algae and Saccharomyces cerevisiae yeast in terms of preconcentration and selective retention of Pt(IV). The immobilized fungi presented here have a high potential for use in platinum biosorption. The procedure exhibits the currently lowest limit of detection (0.02 ng mL(-1) of Pt) and very high selectivity. The procedure was applied to the determination of Pt(IV) in river water, road run-off, and wastewater samples.FigureSchematic diagram of flow injection manifold for on-line preconcentration/separation of Pt(IV) on immobilized fungi followed by its luminol-based chemiluminescent determination. The CL-FIA manifold was applied to the determination of platinum in river water, road run-off, and wastewater samples. PMID:22347728

  13. Flame Chemiluminescence Rate Constants for Quantitative Microgravity Combustion Diagnostics

    NASA Technical Reports Server (NTRS)

    Luque, Jorge; Smith, Gregory P.; Jeffries, Jay B.; Crosley, David R.; Weiland, Karen (Technical Monitor)

    2001-01-01

    Absolute excited state concentrations of OH(A), CH(A), and C2(d) were determined in three low pressure premixed methane-air flames. Two dimensional images of chemiluminescence from these states were recorded by a filtered CCD camera, processed by Abel inversion, and calibrated against Rayleigh scattering, Using a previously validated 1-D flame model with known chemistry and excited state quenching rate constants, rate constants are extracted for the reactions CH + O2 (goes to) OH(A) + CO and C2H + O (goes to) CH(A) + CO at flame temperatures. Variations of flame emission intensities with stoichiometry agree well with model predictions.

  14. Abnormal Magnetic Field Effects on Electrogenerated Chemiluminescence

    NASA Astrophysics Data System (ADS)

    Pan, Haiping; Shen, Yan; Wang, Hongfeng; He, Lei; Hu, Bin

    2015-03-01

    We report abnormal magnetic field effects on electrogenerated chemiluminescence (MFEECL) based on triplet emission from the Ru(bpy)3Cl2-TPrA electrochemical system: the appearance of MFEECL after magnetic field ceases. In early studies the normal MFEECL have been observed from electrochemical systems during the application of magnetic field. Here, the abnormal MFEECL suggest that the activated charge-transfer [Ru(bpy)33+ … TPrA•] complexes may become magnetized in magnetic field and experience a long magnetic relaxation after removing magnetic field. Our analysis indicates that the magnetic relaxation can gradually increase the density of charge-transfer complexes within reaction region due to decayed magnetic interactions, leading to a positive component in the abnormal MFEECL. On the other hand, the magnetic relaxation facilitates an inverse conversion from triplets to singlets within charge-transfer complexes. The inverse triplet --> singlet conversion reduces the density of triplet light-emitting states through charge-transfer complexes and gives rise to a negative component in the abnormal MFEECL. The combination of positive and negative components can essentially lead to a non-monotonic profile in the abnormal MFEECL after ceasing magnetic field. Nevertheless, our experimental studies may reveal un-usual magnetic behaviors with long magnetic relaxation from the activated charge-transfer [Ru(bpy)33+ … TPrA•] complexes in solution at room temperature.

  15. Abnormal magnetic field effects on electrogenerated chemiluminescence.

    PubMed

    Pan, Haiping; Shen, Yan; Wang, Hongfeng; He, Lei; Hu, Bin

    2015-01-01

    We report abnormal magnetic field effects on electrogenerated chemiluminescence (MFEECL) based on triplet emission from the Ru(bpy)3Cl2-TPrA electrochemical system: the appearance of MFEECL after magnetic field ceases. In early studies the normal MFEECL have been observed from electrochemical systems during the application of magnetic field. Here, the abnormal MFEECL suggest that the activated charge-transfer [Ru(bpy)3(3+) … TPrA(•)] complexes may become magnetized in magnetic field and experience a long magnetic relaxation after removing magnetic field. Our analysis indicates that the magnetic relaxation can gradually increase the density of charge-transfer complexes within reaction region due to decayed magnetic interactions, leading to a positive component in the abnormal MFEECL. On the other hand, the magnetic relaxation facilitates an inverse conversion from triplets to singlets within charge-transfer complexes. The inverse triplet ? singlet conversion reduces the density of triplet light-emitting states through charge-transfer complexes and gives rise to a negative component in the abnormal MFEECL. The combination of positive and negative components can essentially lead to a non-monotonic profile in the abnormal MFEECL after ceasing magnetic field. Nevertheless, our experimental studies may reveal un-usual magnetic behaviors with long magnetic relaxation from the activated charge-transfer [Ru(bpy)3(3+) … TPrA(•)] complexes in solution at room temperature. PMID:25772580

  16. Abnormal Magnetic Field Effects on Electrogenerated Chemiluminescence

    PubMed Central

    Pan, Haiping; Shen, Yan; Wang, Hongfeng; He, Lei; Hu, Bin

    2015-01-01

    We report abnormal magnetic field effects on electrogenerated chemiluminescence (MFEECL) based on triplet emission from the Ru(bpy)3Cl2-TPrA electrochemical system: the appearance of MFEECL after magnetic field ceases. In early studies the normal MFEECL have been observed from electrochemical systems during the application of magnetic field. Here, the abnormal MFEECL suggest that the activated charge-transfer [Ru(bpy)33+ … TPrA•] complexes may become magnetized in magnetic field and experience a long magnetic relaxation after removing magnetic field. Our analysis indicates that the magnetic relaxation can gradually increase the density of charge-transfer complexes within reaction region due to decayed magnetic interactions, leading to a positive component in the abnormal MFEECL. On the other hand, the magnetic relaxation facilitates an inverse conversion from triplets to singlets within charge-transfer complexes. The inverse triplet ? singlet conversion reduces the density of triplet light-emitting states through charge-transfer complexes and gives rise to a negative component in the abnormal MFEECL. The combination of positive and negative components can essentially lead to a non-monotonic profile in the abnormal MFEECL after ceasing magnetic field. Nevertheless, our experimental studies may reveal un-usual magnetic behaviors with long magnetic relaxation from the activated charge-transfer [Ru(bpy)33+ … TPrA•] complexes in solution at room temperature. PMID:25772580

  17. A new modular chemiluminescence immunoassay analyser evaluated.

    PubMed

    Ognibene, A; Drake, C J; Jeng, K Y; Pascucci, T E; Hsu, S; Luceri, F; Messeri, G

    2000-03-01

    Thyrotropin (TSH), free thyroxine (fT4) and testosterone assays have been used as a probe to evaluate the performances of a new modular chemiluminescence (CL) immunoassay analyser, the Abbott Architect 2000. The evaluation was run in parallel on other systems that use CL as the detection reaction: DPC Immulite, Chiron Diagnostics ACS-180 and ACS Centaur (TSH functional sensitivity only). TSH functional sensitivity was 0.0012, 0.009, 0.033 and 0.039 mU/I for the Architect, Immulite, ACS Centaur and ACS-180, respectively. Testosterone functional sensitivity was 0.38, 3.7 and 2.0 nmol/l for Architect, Immulite and ACS-180, respectively. Good correlation was obtained between the ACS-180 and Architect for all assays. The Immulite correlation did not agree well with the Architect or ACS-180 for fT4 and testosterone but was in good agreement for TSH. Regarding fT4 and testosterone, equilibrium dialysis and isotopic dilution gas-chromatography mass-spectrometry (GC-MS) respectively were used as reference methods. For both within- and between-run precision, the Architect showed the best reproducibility for all three analytes (CV < 6%). PMID:10905763

  18. Reactivity of chemiluminescence reagents toward oxidants

    SciTech Connect

    Khevelev, M.; Weinstein-Loyd, J.B. [State Univ. of New York, Old Westbury, NY (United States)

    1996-10-01

    Hydroperoxyl radical (HO{sub 2}) and its conjugate base, superoxide radical (O{sub 2}{sup -}) are important chemical intermediates. O{sub 2}{sup -} is ubiquitous in aerobic cells and has been implicated in arthritis, cancer, and aging, among other biological processes. HO{sub 2} plays a central role in atmospheric photochemistry. Because of their short lifetime, there are few reliable analytical methods for the detection of HO{sub 2}/O{sub 2}{sup -}. In a number of recent publications, the chemiluminescence reagent CLA has been exploited as a specific marker for these species. Using UV/visible spectroscopy, we have investigated the stability of CLA and several of its analogs in the presence of oxidants, including O{sub 2}, H{sub 2}O{sub 2}, OH and HO{sub 2}/O{sub 2}{sup -}. The spectral changes observed suggest that the reaction with HO{sub 2}/O{sub 2}{sup -} is rather nonspecific.

  19. Analytical Applications of Bioluminescence and Chemiluminescence

    NASA Technical Reports Server (NTRS)

    Chappelle, E. W. (editor); Picciolo, G. L. (editor)

    1975-01-01

    Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized. Photometer designs are also considered.

  20. HALOCARBON INTERFERENCES IN CHEMILUMINESCENT MEASUREMENTS OF NOX

    EPA Science Inventory

    Anomalous NOx responses were observed when halocarbons were irradiated in the presence of oxides of nitrogen. Interferences to chemiluminescent NOx monitor using heated carbon converter were studied for phosgene, tri-chloroacetyl chloride, chloroform, chlorine, and hydrochloric a...

  1. 5-Amino-4-sulfanylphthalhydrazide as a chemiluminescence derivatization reagent for aromatic aldehydes in liquid chromatography.

    PubMed

    Yoshida, H; Nakao, R; Nohta, H; Yamaguchi, M

    2000-11-10

    5-Amino-4-sulfanylphthalhydrazide (ASPH) was synthesized as a chemiluminescence derivatization reagent for aromatic aldehydes in liquid chromatography (LC). Benzaldehyde, 4-tolualdehyde, 4-chlorobenzaldehyde, 4-formylbenzoic acid, 4-hydroxybenzaldehyde and vanillin were used as model compounds to optimize the derivatization conditions. This reagent, ASPH, reacts selectively with aromatic aldehydes in the presence of sodium sulfite and disodium hydrogenphoshite in acidic medium at 100 degrees C to give the corresponding highly chemiluminescent 2-arylbenzothiazole derivatives. The resulting derivatives generated intense chemiluminescence by reaction with hydrogen peroxide and potassium hexacyanoferrate(III) in alkaline solution. The ASPH derivatives of aromatic aldehydes were separated by reversed-phase liquid chromatography with isocratic elution, and detected chemiluminometrically after mixing with oxidizing agents. The detection limits (signal-to-noise ratio = 3) for aromatic aldehydes are in the range 0.2-4.0 fmol for a 20-microl injection volume. Currently, the method is not effective for aliphatic aldehydes because of interfering LC peaks. PMID:11185615

  2. Long-lived chemiluminescence in cigarette smoke

    Microsoft Academic Search

    H. H. Seliger; W. H. Biggley; J. P. Hamman

    1974-01-01

    Cigarette smoke contains high concentrations of unstable molecules that react with oxygen to produce chemiluminescence. The chemiluminescent activity concentrated in the aerosol phase that can be absorbed on glass-fiber filters and extracted into organic solvents. Cigarette smoke in N,N-dimethylformamide produces a long-lasting luminescence visible to the dark-adapted eye. We have demonstrated the oxygen dependence and have measured the kinetics, activation

  3. Multicolor Directional Surface Plasmon-Coupled Chemiluminescence

    PubMed Central

    Chowdhury, Mustafa H.; Malyn, Stuart N.; Aslan, Kadir; Lakowicz, Joseph R.; Geddes, Chris D.

    2009-01-01

    In reports over the past several years, we have demonstrated the efficient collection of optically excited fluorophore emission by its coupling to surface plasmons on thin metallic films, where the coupled luminescence was highly directional and polarized. This phenomenon is referred to as surface plasmon-coupled emission (SPCE). In this current study, we have extended this technique to include chemiluminescing species and subsequentially now report the observation of surface plasmon-coupled chemiluminescence (SPCC), where the luminescence from chemically induced electronic excited states couples to surface plasmons in thin continuous metal films. The SPCC is highly directional and predominantly p-polarized, strongly suggesting that the emission is from surface plasmons instead of the luminophores themselves. This indicates that surface plasmons can be directly excited from chemically induced electronic excited states and excludes the possibility that the plasmons are created by incident excitation light. This phenomenon has been observed for a variety of chemiluminescent species in the visible spectrum, ranging from blue to red, and also on a variety of metals, namely, aluminum, silver, and gold. Our findings suggest new chemiluminescence sensing strategies on the basis of localized, directional, and polarized chemiluminescence detection, especially given the wealth of assays that currently employ chemiluminescence-based detection. PMID:17092012

  4. Human saliva-based quantitative monitoring of clarithromycin by flow injection chemiluminescence analysis: a pharmacokinetic study.

    PubMed

    Tan, Xijuan; Song, Zhenghua

    2014-02-01

    Human saliva quantitative monitoring of clarithromycin (CLA) by chemiluminescence (CL) with flow injection analysis was proposed for the first time, which was based on the quenching effect of CLA on luminol-bovine serum albumin (BSA) CL system with a linear range from 7.5 × 10(-4) to 2.0 ng/ml. This proposed approach, offering a maximum sample throughput of 100 h(-1), was successfully applied to the quantitative monitoring of CLA levels in human saliva during 24 h after a single oral dose of 250 mg intake, with recoveries of 95.2 ? 109.0% and relative standard deviations lower than 6.5 % (N = 7). Results showed that CLA reached maximum concentration of 2.28 ± 0.02 ?g/ml at approximately 3 h, and the total elimination ratio was 99.6 % in 24 h. The pharmacokinetic parameters including absorption rate constant (0.058 ± 0.006 h(-1)), elimination rate constant (0.149 ± 0.009 h(-1)) and elimination half-life time (4.66 ± 0.08 h) were obtained. A comparison of human saliva and urine monitoring was also given. The mechanism study of BSA-CLA interaction revealed the binding of CLA to BSA is an entropy driven and spontaneous process through hydrophobic interaction, with binding constant K BSA-CLA of 4.78 × 10(6) l/mol and the number of binding sites n of 0.82 by flow injection-chemiluminescence model. Molecular docking analysis further showed CLA might be in subdomain IIA of BSA, with K BSA-CLA of 6.82 × 10(5) l/mol and ?G of -33.28 kJ/mol. PMID:24166104

  5. Enhanced electrochemiluminescence from luminol at carboxyl graphene for detection of ?-fetoprotein.

    PubMed

    Li, Xiaojian; Guo, Qingfang; Cao, Wei; Li, Yueyun; Du, Bin; Wei, Qin

    2014-07-15

    In this study, a novel sensitive electrochemiluminescence (ECL) immunosensor was constructed by carboxyl graphene (GR) for enhancing luminol-O2 system emission. Here, carboxyl GR was used to enhance the ECL intensity of luminol that had excellent electron transfer ability and good solubility. The sensing platform was constructed by depositing carboxyl GR on electrodes and immobilizing antibodies on the surface of carboxyl GR through amidation. The specific immunoreaction between ?-fetoprotein (AFP) and antibodies resulted in a decrease of ECL intensity, and the intensity decreased linearly with AFP concentrations in the range of 5 pg ml(-1) to 14 ng ml(-1) with a detection limit of 2.0 pg ml(-1). The proposed immunosensor exhibits high specificity, good reproducibility, and longtime stability. It may become a promising technique for protein detection. PMID:24769071

  6. Volume 189, number 4.5 CHEMICAL PHYSICS LETTERS 14 February 1992 A crossed beam study of the reaction C (3P ) + N20

    E-print Network

    Reisler, Hanna

    of chemiluminescent products un- der rotationally relaxed conditions. In addition, de- tection by laser+)+CN(A `l-I)+NO(X `I-I), AH=-1.47eV. (4) Chemiluminescence from the electronically excited products of reactions ( 1) and (2 ) has not been ob- served, while CN(A+X) chemiluminescence from reaction (4) has been

  7. [A comparison of the Bluestar and luminol effectiveness in bloodstain detection].

    PubMed

    ?uczak, Sylwia; Wo?niak, Marcin; Papuga, Marta; Stopi?iska, Katarzyna; Sliwka, Karol

    2006-01-01

    The objective of the present study was to compare the effectiveness of two chemical agents--Bluestar and luminol--in detection of bloodstains. The experiments were performed to test for bloodstain detection sensitivity, chemical stability and to investigate the effect of both reagents on DNA typing. During this study, the authors prepared serial dilutions (1:2 to 1:10 000 000) of fresh blood, as well as dilutions of 25-year old blood on Whatman 3MM blotting paper. Additional dilutions of fresh blood were spotted on a glass surface. The experiments showed very similar results for both investigated reagents, although the Bluestar solution proved to be more stable (at least 7 days after the preparation) as compared to luminol (stable for not more than 24 hours). Both reagents showed a higher sensitivity for diluted bloodstains on a glass surface than for similar stains on filter paper. The investigators also demonstrated that multiplex amplification of DNA was feasible after Bluestar or luminol treatment, although the detected bloodstains might be too diluted to allow for effective DNA extraction and amplification. PMID:17249372

  8. Ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging.

    PubMed

    Wang, Chaoguang; Xiao, Rui; Dong, Peitao; Wu, Xuezhong; Rong, Zhen; Xin, Lin; Tang, Jun; Wang, Shengqi

    2014-07-15

    This paper describes a rapid, ultra-sensitive, and high-throughput pathogenic DNA identification strategy for infectious diarrheal diseases diagnosis. This strategy is based on specific DNA hybridization and horseradish-peroxidase-catalyzed chemiluminescence (CL) detection. Probe DNA strands are covalently immobilized on the aldehyde-group-modified slide and hybridized with biotin-modified target DNA strands. Horseradish-peroxidase (HRP) is then combined with the target DNA via a biotin-streptavidin linkage. The subsequently added mixture of luminol and hydrogen peroxide is catalyzed by HRP and radiates photons. The photons are collected and read out by a portable imager. The specific detection of target DNA strands was realized at a detection limitation of about 0.75 nM. This strategy facilitates quantitative detection, as indicated by the fact that the CL signals were consistent well with a linear function. This method was applied to identify a myriad of real diarrheal pathogens samples, including Enterohemorrhagic Escherichia coli (EHEC), Vibrio cholerae (VBC), Shigella (SHLA), and Salmonella (SMLA). Triple-assay of six gene sequences from these pathogens was realized, which facilitates accurate, high-throughput identification of diarrheal pathogens. This CL assay strategy is appropriate for application in disease diagnosis and prevention. PMID:24534578

  9. A quantitative chemiluminescent assay for analysis of peroxide-based explosives.

    PubMed

    Girotti, S; Ferri, E; Maiolini, E; Bolelli, L; D'Elia, M; Coppe, D; Romolo, F S

    2011-04-01

    A quantitative chemiluminescent method, enabling indirect identification of the peroxide-based explosives TATP (triacetone triperoxide) and HMTD (hexamethylene triperoxide diamine) has been developed. Treatment of these compounds with acidic solutions produced peroxides, which were transformed into radical derivatives by horseradish peroxidase (HRP) and then quantified by measuring the light emitted during their oxidation of luminol. The method was first developed in the microplate format and later optimized for a portable luminometer, to enable rapid application of the assay directly on site. When the portable luminometer was used each analysis took only 5-10 min. The method had good selectivity, sensitivity, and reproducibility; in the microplate format the limits of detection (LOD) and quantification (LOQ) were 40 and 50 ng mL(-1), respectively, for both TATP and HMTD. When the portable luminometer was used the LOD and LOQ were 50 and 100 ng mL(-1), respectively, for both compounds. Introduction of light emission-enhancing compounds did not improve the analytical performance of the assay. Imprecision (CV values) was always below 10%. Recovery varied rapidly with time, with an average value of 78% after 5 min. No false-positive result was detected on measurement of a variety of samples; this is an important feature for analysis on site. The method was applied both to contaminated materials and to fortified soil samples, simulating operational conditions. PMID:21249343

  10. Microplate analytical method for quinones by pulse photo-irradiation and chemiluminescence detection.

    PubMed

    Elgawish, Mohamed Saleh; Shimomai, Chikako; Kishikawa, Naoya; Ohyama, Kaname; Nakashima, Kenichiro; Kuroda, Naotaka

    2012-10-21

    Quinones are widely distributed in nature and have various bioactivities. Besides, quinones are also considered as toxicological intermediates which cause severe dangerous effects. Hereby, a sensitive, simple, and rapid method is reported for quinones determination. The proposed method employed time resolved fluorescence (TRF) microplate reader based chemiluminescent (CL) detection for the first time as a novel approach for measurement. Under pulse photo-irradiation, the unique photochemical characteristic of quinones is exploited to liberate reactive oxygen species (ROS) which reacted with photosensitized CL reagent. L-012, luminol analogue, was selected for its high sensitivity. Under our investigation, para-quinones showed high CL response when compared to ortho-quinones. A linear response was obtained for studied quinone concentrations in the range of 0.05-50 ?M for 1,4-naphthquinone and of 0.05-150 ?M for 2-methyl-1,4-naphthoquinone (menadione) and 9,10-anthraquinone with detection limit (blank + 3SD) of 0.01 ?M. The proposed method allowed the rapid determination of large number of samples in very short time (96 sample/125 s). The proposed method was successfully applied for determination of menadione in spiked human serum. PMID:22910835

  11. STUDY OF INTERFERENCES IN OZONE UV AND CHEMILUMINESCENCE MONITORS

    EPA Science Inventory

    A study was conducted to examine interferences and other measurement anomalies in chemiluminescence and ultraviolet ozone monitors. revious results had shown that there was a positive deviation in the chemiluminescence monitors and no direct interference with ultraviolet monitors...

  12. Determination of norfloxacin using gold nanoparticles catalyzed cerium(IV)–sodium sulfite chemiluminescence

    Microsoft Academic Search

    Xijuan Yu; Junfang Bao

    2009-01-01

    A rapid flow-injection chemiluminescence (CL) method is proposed for the determination of norfloxacin (NFLX). The method is based on the fact that the weak CL from the redox reaction of Ce(IV)–Na2SO3 can be greatly strengthened by gold nanoparticles (NPs). UV–visible spectra, fluorescence spectra and transmission electron microscopy (TEM) studies are carried out before and after the CL reactions to investigate

  13. [Comparison of chemiluminescence and polynuclear neutrophil count after aplasia in hematologic malignancies].

    PubMed

    Cheze, S; Macro, M; Reman, O; Levaltier, X; Penther, D; Leporrier, M; Troussard, X

    1996-10-01

    Polymorphonuclear neutrophils (PNN) chemiluminescence results from the luminescence emission in oxidative reactions during phagocytosis. This activity is easily detectable and measurable using a luminometer. PNN luminescence allows precociously the evaluation of the end of the aplasia. We have analysed PNN luminescence emission stimulated by latex beads in 69 patients with hemopathies after 72 aplasias following autologous bone marrow transplantation (9 pts), allogeneic bone marrow transplantation (26 pts) and acute myeloblastic leukemia (AML) induction chemotherapies (37 pts). Luminescence emission was measured in whole blood using a luminometer before, during and after aplasia and was compared to PNN manual count. Chemiluminescence measurement is a simple and reproductable method. Its allows more earlier than the PNN blood count the detection of PNN recovery: chemiluminescence nadir is reached on average on the fourth day of aplasia and correspond to the value procured by sample tubes without PNN. The onset of the chemiluminescence increasing is definite by the doubling of the nadir value. It is reached on average on the fourteenth day of aplasia. It precedes 3.5 days and 10.5 days a total PNN count > or = 0.1 x 10 9/1 and > or = 0.5 x 10 9/1 respectively, in the 72 aplasias. In autologous and allogeneic bone marrow transplantation, chemiluminescence increasing precedes at least PNN > or = 0.1 x 10 9/1 of 4.6 days whereas in AML induction chemotherapies, the advantage is only 1.5 day (p = 0.0078). The chemiluminescence could be considered as an additional tool in daily management of sustained aplasia. PMID:8977929

  14. CuO nanosheets-enhanced flow-injection chemiluminescence system for determination of vancomycin in water, pharmaceutical and human serum

    NASA Astrophysics Data System (ADS)

    Khataee, A. R.; Hasanzadeh, A.; Iranifam, M.; Fathinia, M.; Hanifehpour, Y.; Joo, S. W.

    2014-03-01

    A novel, rapid and sensitive CuO nanosheets (NSs) amplified flow-injection chemiluminescence (CL) system, luminol-H2O2-CuO nanosheets, was developed for determination of the vancomycin hydrochloride for the first time. It was found that vancomycin could efficiently inhibit the CL intensity of luminol-H2O2-CuO nanosheets system in alkaline medium. Under the optimum conditions, the inhibited CL intensity was linearly proportional to the concentration of vancomycin over the ranges of 0.5-18.0 and 18.0-40.0 mg L-1, with a detection limit (3?) of 0.1 mg L-1. The precision was calculated by analyzing samples containing 5.0 mg L-1 vancomycin (n = 11) and the relative standard deviation (RSD) was 2.8%. Also, a high injection throughput of 120 sample h-1 was obtained. The CuO nanosheets were synthesized by a sonochemical method. Also, X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses were employed to characterize the CuO nanosheets. The method was successfully employed to determine vancomycin hydrochloride in environmental water samples, pharmaceutical formulation and spiked human serum.

  15. Using the FL600 Fluorescence Microplate Reader for Chemiluminescence Detection

    E-print Network

    Raizada, Manish N.

    Using the FL600 Fluorescence Microplate Reader for Chemiluminescence Detection What's a lumin anyway? Chemiluminescence is a highly sensitive technique that has been employed in a wide variety microplate reader for "glow" chemiluminescent determinations. Introduction Molecules that release light do so

  16. AIAA 2003-4490 Chemiluminescence Based Sensors for

    E-print Network

    Seitzman, Jerry M.

    AIAA 2003-4490 Chemiluminescence Based Sensors for Turbine Engines T. M. Muruganandam, B. Kim, R of Aeronautics and Astronautics, Inc. with permission. Chemiluminescence Based Sensors for Turbine Engines T. M for natural gas/methane combustion above 3 atm. Second, chemiluminescence emission from the combustor was used

  17. Microemulsion-enhanced electrochemiluminescence of luminol-H2O2 for sensitive flow injection analysis of antioxidant compounds.

    PubMed

    Xiuhua, Wei; Chao, Liu; Yifeng, Tu

    2012-05-30

    A microemulsion enhanced electrochemiluminescence (ECL) of luminol-H(2)O(2) was studied with the flow-injection (FI) technique. The results revealed that the microemulsion composed with cetyltrimethylammonium bromide (CTAB), n-butanol, n-heptane and water greatly enhanced the ECL especially in acidic medium. The ECL emission increased for 20 to 2 times in this microemulsion medium over the pH range of 5.0-8.0 compared to that in aqueous solution. The mechanism of enhancement of surfactant and microemulsion for luminol-H(2)O(2) ECL was discussed. It is mainly based on the electrostatic interaction between luminol anion and the head group of surfactant, which causes the adsorption and promotes the dissociation of luminol on the surfaces of the microemulsion droplets, favors the oxidation of luminol by the yielded reactive oxygen species (ROSs) during electrolysis. This research is very significant for ECL applications because of the extended practicable pH range which was suitable for environmental and biological systems. As an example, this FI-ECL technique can be applied for determination of oligo proanthocyanidin (OPC) because of its antioxidant property and to evaluate the total antioxidant activity of the grape skin using OPC as an index. PMID:22608450

  18. Chemiluminescence detection of amino acids, peptides, and proteins using tris-2,2 prime -bipyridine ruthenium(III)

    SciTech Connect

    Li He; Cox, K.A.; Danielson, N.D. (Miami Univ., Oxford, OH (United States))

    1990-01-01

    The feasibility of using the tris-2-2{prime}-bipyridine ruthenium(III) chemiluminescent (CL) reaction for the detection of amino acids, peptides, and proteins has been studied. Detection limits of the amino acids as determined by flow injection analysis (FIA) ranged from 20 pmol of proline to 50 nmol of asparagine. In general, amino acids containing secondary amine groups yielded the strongest responses. A reaction mechanism for Ru(bpy){sub 3}{sup 3+} chemiluminescence of aliphatic amines has been proposed. Studies of peptide molecules and poly-prolines showed that the peptide bond barely contributes to the detection signals. The separation of hydroxyproline and proline in synthetic collagen by HPLC with Ru(bpy){sub 3}{sup 3+} chemiluminescence detection has been shown to be possible.

  19. Electrogenerated chemiluminescence. 59. Rhenium complexes

    SciTech Connect

    Richter, M.M.; Debad, J.D.; Bard, A.J. [Univ. of Texas, Austin, TX (United States)] [Univ. of Texas, Austin, TX (United States); Striplin, D.R.; Crosby, G.A. [Washington State Univ., Pullman, WA (United States)] [Washington State Univ., Pullman, WA (United States)

    1996-12-15

    Re(L)(CO){sub 3}Cl complexes (where L is 1,10-phenanthroline, 2,2`-bipyridine, or a phenanthroline or bipyridine derivative containing methyl groups) are photoluminescent in fluid solution at room temperature. In acetonitrile solutions, these complexes display one chemically reversible one-electron reduction process and one chemically irreversible oxidation process. {lambda}{sub max} for the luminescence is dependent on the nature of L, and a linear relationship between {lambda}{sub max} and the difference in electrode potentials for oxidation and reduction is evident. Electrogenerated chemiluminescence (ECL) was observed in acetonitrile solutions of these complexes (Bu{sub 4}NPF{sub 6} as electrolyte) by stepping the potential of a Pt disk working electrode between potentials sufficient to form the radical anionic and cationic species. The relative amount of light produced during the anodic and cathodic pulses was dependent on the potential limits and pulse duration. ECL was also generated in the presence of coreactants, i.e., with tri-n-propylamine upon stepping the potential sufficiently positive to form the deprotonated tri-n-propylamine radical and the cationic rhenium(II) species Re{sup II}(L)(CO){sub 3}Cl{sup +}. When S{sub 2}O{sub 8}{sup 2-} was present in solution, ECL was also observed for all of the complexes upon stepping to potentials sufficient to form (Re{sup I}(L)(CO){sub 3}Cl){sup -} and the strong oxidant SO{sub 4}{sup .-}. 44 refs., 8 figs.

  20. Low-level chemiluminescence and life span of Drosophila melanogaster.

    PubMed

    Sato, T; Miyazawa, T; Kobayashi, M; Furukawa, H; Inaba, H

    1992-01-01

    Spontaneous photon emission (chemiluminescence, CL) as a monitor of free radical evolution in Drosophila melanogaster which had been maintained at 25 or 30 degrees C for 5 days after emergence was measured. When maintained at 30 degrees C the fly CL intensity was stronger than at 25 degrees C. Under the condition of the higher temperature, the fly life span was shorter (mean life span = 29 days at 30 degrees C and 63 days at 25 degrees C), and oxygen consumption (3.7 microliters/mg.h at 25 degrees C, 4.9 microliters/mg.h at 30 degrees C) and the mobility (movement distance = 25 mm/min at 25 degrees C, 700 mm/min at 30 degrees C) increased, together with augmentation of phospholipid hydroperoxide in the fly total lipids. The CL spontaneously emitted from fly homogenate was decreased by the free radical scavengers both in experiments in vivo and in vitro. The hypothesis is proposed that as the oxygen metabolism grows active, the chemiluminescent reactions that involve oxygen-dependent free radical metabolism, including membrane phospholipid hydroperoxidation, contribute to the acceleration of senescence of fly bodies. PMID:1612461

  1. Highly sensitive flow-injection chemiluminescence determination of pyrogallol compounds

    NASA Astrophysics Data System (ADS)

    Kanwal, Shamsa; Fu, Xiaohong; Su, Xingguang

    2009-12-01

    A highly sensitive flow-injection chemiluminescent method for the direct determination of pyrogallol compounds has been developed. Proposed method is based on the enhanced effect of pyrogallol compounds on the chemiluminescence signals of KMnO 4-H 2O 2 system in slightly alkaline medium. Three important pyrogallol compounds, pyrogallic acid, gallic acid and tannic acid, have been detected by this method, and the possible mechanism of the CL reaction is also discussed. The proposed method is simple, convenient, rapid (60 samples h -1), and sensitive, has a linear range of 8 × 10 -10 mol L -1 to 1 × 10 -5 mol L -1, for pyrogallic acid, with a detection limit of 6 × 10 -11 mol L -1, 4 × 10 -8 mol L -1 to 5 × 10 -3 mol L -1 for gallic acid with a detection limit of 9 × 10 -10 mol L -1, and 8 × 10 -8 mol L -1 to 5 × 10 -2 mol L -1 for tannic acid, with a detection limit of 2 × 10 -9 mol L -1, respectively. The relative standard deviation (RSD, n = 15) was 0.8, 1.1 and 1.3% for 5 × 10 -6 mol L -1 pyrogallic acid, gallic acid and tannic acid, respectively. The proposed method was successfully applied to the determination of pyrogallol compounds in tea and coffee samples.

  2. Chemiluminescence assay for the detection of biological warfare agents

    SciTech Connect

    Langry, K; Horn, J

    1999-11-05

    A chemiluminescent homogeneous immunoassay and a hand-size multiassay reader are described that could be used for detecting biological materials. The special feature of the assay is that it employs two different antibodies that each bind to a unique epitope on the same antigen. Each group of epitope-specific antibodies has linked to it an enzyme of a proximal-enzyme pair. One enzyme of the pair utilizes a substrate in high concentration to produce a second substrate required by the second enzyme. This new substrate enables the second enzyme to function. The reaction of the second enzyme is configured to produce light. This chemiluminescence is detected with a charge-coupled device (CCD) camera. The proximal pair enzymes must be in close proximity to one another to allow the second enzyme to react with the product of the first enzyme. This only occurs when the enzyme-linked antibodies are attached to the antigen, whether antigen is a single protein with multiple epitopes or the surface of a cell with a variety of different antigens. As a result of their juxtaposition, the enzymes produce light only in the presence of the biological material. A brief description is given as to how this assay could be utilized in a personal bio-agent detector system.

  3. Chemiluminescence Study of the Autoxidation of cis-1,4-Polyisoprene

    NASA Technical Reports Server (NTRS)

    Mendenhall, G. David; Nathan, Richard A.; Golub, Morton A.

    1978-01-01

    The free-radical mechanism for the autoxidation of cis-1,4-polyisoprene (natural rubber or its synthetic counterpart) has been investigated extensively. An important feature of this mechanism, and indeed also of the autoxidation of hydrocarbons generally, is that it is a chain process propagated by alkyl and peroxy radicals and terminated through bimolecular reactions involving these same radicals. In the usual oxidation situation, that is, at all oxygen pressures greater than a few torr, the alkyl radicals are rapidly converted to peroxy radicals, and the termination step proceeds almost exclusively through the latter radicals. The bimolecular decay of the peroxy radicals is accompanied by a weak emission of light or chemiluminescence. Kinetic evidence is consistent with an electronically excited ketone produced in the termination reaction as the source of the emission. The first observation of chemiluminescence from the oxidative degradation of polymers was reported by Ashby, who dealt mainly with polypropylene but made passing mention of several other polymers. Subsequently, a number of papers have appeared dealing with oxidative chemiluminescence from a variety of polymers. In this paper we report the first detailed study of the chemiluminescence emitted in the autoxidation of cis-1,4-polyisoprene. The chemiluminescence technique is extremely sensitive and can follow rates of oxidation that are too slow to be measured conveniently by other means. This work thus offered the potential of throwing new light on the autoxidation of cis-1,4-polyisoprene, especially in the very early stages or under ambient conditions where conventional spectroscopic procedures are rather insensitive.

  4. On the interaction of luminol with human serum albumin: Nature and thermodynamics of ligand binding

    NASA Astrophysics Data System (ADS)

    Moyon, N. Shaemningwar; Mitra, Sivaprasad

    2010-09-01

    The mechanism and thermodynamic parameters for the binding of luminol (LH 2) with human serum albumin was explored by steady state and picosecond time-resolved fluorescence spectroscopy. It was shown that out of two possible LH 2 conformers present is solution, only one is accessible for binding with HSA. The thermodynamic parameters like enthalpy (? H) and entropy (? S) change corresponding to the ligand binding process were also estimated by performing the experiment at different temperatures. The ligand replacement experiment with bilirubin confirms that LH 2 binds into the sub-domain IIA of the protein.

  5. Measurement of soil/dust arsenic by gas phase chemiluminescence.

    PubMed

    Sawalha, Maather F; Sengupta, Mrinal K; Ohira, Shin-Ichi; Idowu, Ademola D; Gill, Thomas E; Rojo, Lila; Barnes, Melanie; Dasgupta, Purnendu K

    2008-10-19

    A gas phase chemiluminescence (GPCL)-based method for trace measurement of arsenic has been recently described for the measurement of arsenic in water. The principle is based on the reduction of inorganic As to AsH(3) at a controlled pH (the choice of pH governs whether only As(III) or all inorganic As is converted) and the reaction of AsH(3) with O(3) to produce chemiluminescence (Idowu et al., Anal. Chem. 78 (2006) 7088-7097). The same general principle has also been used in postcolumn reaction detection of As, where As species are separated chromatographically, then converted into inorganic As by passing through a UV photochemical reactor followed by AsH(3) generation and CL reaction with ozone (Idowu and Dasgupta, Anal. Chem. 79 (2007) 9197-9204). In the present paper we describe the measurement of As in different soil and dust samples by serial extraction with water, citric acid, sulfuric acid and nitric acid. We also compare parallel measurements for total As by induction coupled plasma mass spectrometry (ICP-MS). As(V) was the only species found in our samples. Because of chloride interference of isobaric ArCl(+) ICP-MS analyses could only be carried out by standard addition; these results were highly correlated with direct GPCL and LC-GPCL results (r(2)=0.9935 and 1.0000, respectively). The limit of detection (LOD) in the extracts was 0.36 microg/L by direct GPCL compared to 0.1 microg/L by ICP-MS. In sulfuric acid-based extracts, the LC-GPCL method provided LODs inferior to those previously observed for water-based standards and were 2.6, 1.3, 6.7, and 6.4 microg/L for As(III), As(V), dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA), respectively. PMID:18804648

  6. OPTIMIZED CHEMILUMINESCENCE SYSTEM FOR MEASURING ATMOSPHERIC AMMONIA

    EPA Science Inventory

    The optimization and testing of a continuous measurement system for analyzing atmospheric ammonia concentrations (0 to 10 ppb) is described. The measurement system combines an ultra-sensitive chemiluminescence nitric oxide detector, with a thermal converter for NH3 to nitric oxid...

  7. Illustrating Chemiluminescence with Siloxene Indicator.

    ERIC Educational Resources Information Center

    Hoff, Ray

    1981-01-01

    Discusses the nature of light-producing reactions and provides a procedure for demonstrating chemical luminescence using siloxene indicator. Indicates source of this chemical and safety precautions. (SK)

  8. Egg-Citing! Isolation of Protoporphyrin IX from Brown Eggshells and Its Detection by Optical Spectroscopy and Chemiluminescence

    ERIC Educational Resources Information Center

    Dean, Michelle L.; Miller, Tyson A.; Bruckner, Christian

    2011-01-01

    A simple and cost-effective laboratory experiment is described that extracts protoporphyrin IX from brown eggshells. The porphyrin is characterized by UV-vis and fluorescence spectroscopy. A chemiluminescence reaction (peroxyoxalate ester fragmentation) is performed that emits light in the UV region. When the porphyrin extract is added as a fluor…

  9. Self-assembly of organogels via new luminol imide derivatives: diverse nanostructures and substituent chain effect

    PubMed Central

    2013-01-01

    Luminol is considered as an efficient sycpstem in electrochemiluminescence (ECL) measurements for the detection of hydrogen peroxide. In this paper, new luminol imide derivatives with different alkyl substituent chains were designed and synthesized. Their gelation behaviors in 26 solvents were tested as novel low molecular mass organic gelators. It was shown that the length and number of alkyl substituent chains linked to a benzene ring in gelators played a crucial role in the gelation behavior of all compounds in various organic solvents. Longer alkyl chains in molecular skeletons in present gelators are favorable for the gelation of organic solvents. Scanning electron microscope and atomic force microscope observations revealed that the gelator molecules self-assemble into different micro/nanoscale aggregates from a dot, flower, belt, rod, and lamella to wrinkle with change of solvents. Spectral studies indicated that there existed different H-bond formations and hydrophobic forces, depending on the alkyl substituent chains in molecular skeletons. The present work may give some insight to the design and characteristic of new versatile soft materials and potential ECL biosensors with special molecular structures. PMID:23758979

  10. Analytical applications of tris(2,2?-bipyridyl)ruthenium(III) as a chemiluminescent reagent

    Microsoft Academic Search

    Richard D. Gerardi; Neil W. Barnett; Simon W. Lewis

    1999-01-01

    This paper reviews the analytical applications of the chemiluminescence reactions involving tris(2,2?-bipyridyl)ruthenium(III), from the earliest paper in 1978 to mid 1998. After an introduction which briefly describes historical perspectives, spectroscopic and mechanistic considerations, the review is divided into two major sections. The first section discusses the methods of generation of tris(2,2?-bipyridyl)ruthenium(III) reagent, including chemical\\/photochemical, electrochemical and in situ electrochemiluminescence methods.

  11. A study on the selection of chemiluminescence system for the flow injection determination of the total polyphenol index of plant-derived foods.

    PubMed

    Nalewajko-Sieliwoniuk, Edyta; Malejko, Julita; ?wi?czkowska, Marta; Kowalewska, Agata

    2015-06-01

    Different chemiluminescence systems based on luminol, permanganate, manganese(IV) and cerium(IV) reagents were compared regarding their sensitivity and selectivity to determine plant polyphenols. Among the seventeen systems tested, Mn(IV)-formaldehyde-hexametaphosphate was considered to be the most suitable for polyphenols detection. The developed flow injection method (FI-CL) based on enhancing effect of polyphenols on Mn(IV) chemiluminescence is characterised by low detection limit of gallic acid (0.02?gL(-1)) and high precision (RSD=1.7%). The calibration graph was linear from 0.1 to 100?gL(-1). The selectivity studies revealed that the FI-CL method ensures accurate determination of the total polyphenols content in food samples. The method was successfully applied to analysis of a variety of plant-derived foods (wine, tea, cereal coffee, fruit and vegetable juices, herbs and spices). The proposed method is superior to conventional spectrophotometric assays due to its higher sample throughput (195samplesh(-1)), simplicity, sensitivity and, above all, higher selectivity. PMID:25624221

  12. Solvent extraction coupled on-line to a reversed micellar mediated chemiluminescence detection system for trace-level determination of atropine.

    PubMed

    Fujiwara, T; Mohammadzai, I U; Murayama, K; Kumamaru, T

    2000-04-01

    A fast and sensitive method for the determination of atropine, an alkaloid closely related to cocaine, is proposed. The principles of on-line ion-pair formation of alkaloid-metal complexes and liquid-liquid extraction are applied to the chemiluminescence determination of atropine. On mixing with a reversed micellar medium of cetyltrimethylammonium chloride in dichloromethane-cyclohexane (1:1 v/v)-water (0.3 M Na2CO3) containing luminol, the ion-pair complex of tetrachloroaurate(III) with atropinium produced an analytical chemiluminescence signal when it entered the reversed micellar water pool. Using the reverse-flow injection and chemical conditions optimized for atropine in aqueous samples, a detection limit of 1 ng/mL was achieved and a linear calibration graph was obtained with a wide dynamic range from 10 ng/mL to 100 micrograms/mL. The proposed method is simple and provides a good precision with a relative standard deviation (n = 6) of ca. 3% at the atropine concentration of 100 ng/mL. After a preliminary study involving the potential interference from species of organic, inorganic, and metallic nature, the method was applied to the determination of atropine in artificial urine samples and of atropine and scopolamine in pharmaceutical formulations. PMID:10763275

  13. Xanthene dye chemiluminescence for determination of free chlorine in water

    SciTech Connect

    Yamada, M.; Hobo, T.; Suzuki, S.

    1988-10-01

    Preliminary investigations by a batch method are described for aiming at the flow determination of free chlorine in water with novel chemiluminescence (CL) detection. The CL originates from the reaction of xanthene dyes with free chlorine, Cl/sub 2/, HOCl, and OCl/sup -/. Through the measurements of CL decay curves, fundamental CL characteristics were explored from the analytical point of view. Among xanthene dyes tested, eosin Y, eosin B, pyronin B, and rhodamine 6G were found to be promising CL reagents with such sensitivity and selectivity that free chlorine can be readily determined in tap water. In particular, these CL systems have the special advantage of being insensitive to oxo acids of chlorine and chloramine. Recommended flow systems are proposed.

  14. In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles

    NASA Astrophysics Data System (ADS)

    Lee, Dongwon; Khaja, Sirajud; Velasquez-Castano, Juan C.; Dasari, Madhuri; Sun, Carrie; Petros, John; Taylor, W. Robert; Murthy, Niren

    2007-10-01

    The overproduction of hydrogen peroxide is implicated in the development of numerous diseases and there is currently great interest in developing contrast agents that can image hydrogen peroxide in vivo. In this report, we demonstrate that nanoparticles formulated from peroxalate esters and fluorescent dyes can image hydrogen peroxide in vivo with high specificity and sensitivity. The peroxalate nanoparticles image hydrogen peroxide by undergoing a three-component chemiluminescent reaction between hydrogen peroxide, peroxalate esters and fluorescent dyes. The peroxalate nanoparticles have several attractive properties for in vivo imaging, such as tunable wavelength emission (460-630nm), nanomolar sensitivity for hydrogen peroxide and excellent specificity for hydrogen peroxide over other reactive oxygen species. The peroxalate nanoparticles were capable of imaging hydrogen peroxide in the peritoneal cavity of mice during a lipopolysaccharide-induced inflammatory response. We anticipate numerous applications of peroxalate nanoparticles for in vivo imaging of hydrogen peroxide, given their high specificity and sensitivity and deep-tissue-imaging capability.

  15. Weak chemiluminescence of bilirubin and its stimulation by aldehydes.

    PubMed

    Watanabe, H; Usa, M; Kobayashi, M; Agatsuma, S; Inaba, H

    1992-01-01

    Bilirubin in an alkaline solution exhibits a weak chemiluminescence (CL) under aerobic conditions. This spontaneous CL was markedly enhanced by the addition of various aldehydes. The fluorescent emission spectrum of bilirubin, excited by weak intensity light at 350 nm, coincided with its CL emission spectrum (peak at 670 nm). CL emission from bilirubin was not quenched by active oxygen scavengers. This suggests that triplet oxygen reacts with bilirubin, and forms an oxygenated intermediate (hydroperoxide) as a primary emitter (oxidative scission of tetrapyrrole bonds in bilirubin is not involved in this CL). The Ehrlich reaction (test for monopyrroles) and hydrolsulphite reaction (test for dipyrroles) on the CL reaction mixture and unreacted bilirubin showed no differences. When the CL was initiated by singlet oxygen, rather than superoxide anion, monopyrrole, was detected in the reaction products by gel chromatography. The inhibitory effect of a scavenger of singlet oxygen on CL was eliminated in the presence of formaldehyde. Therefore, triplet carbonyl, formed by singlet oxygen through the dioxetane structure in bilirubin, is not an emitter. The reaction mechanism of bilirubin CL and the formation of a hydroperoxide intermediate is discussed in relation to the chemical structure of luciferin molecules from bioluminescent organisms. PMID:1322632

  16. Ionization and chemiluminescence during the progressive aeration of methane flames

    SciTech Connect

    Weinberg, Felix; Carleton, Fred [Imperial College London, London SW7 2AZ (United Kingdom)

    2009-12-15

    Saturation currents and chemiluminescence, especially at the CH{sup *} and C{sub 2}{sup *} wavelengths, are measured for a range of small, laminar methane flames during progressive addition of air, with the principal objective of distinguishing between pure diffusion flames, premixed flames of compositions falling between the upper and lower flammability limits, and the broad range of aerated flames lying in between these regimes. Flame areas defined by the loci of maximum luminosity and by schlieren contours were recorded, so that saturation current densities, CH{sup *} and C{sub 2}{sup *} emission per unit flame area, as well as burning velocities could be deduced. For admixtures of less than 70 vol.%, air appears to act, surprisingly, as an inert diluent as regards saturation currents, so that saturation currents are essentially proportional to fuel flow alone. Much the same applies to chemiluminescence. However, schlieren contours, which were recorded both to provide a basis for burning velocity measurements and to explore density changes in the reactants, indicated the presence of a burner - stabilised propagating reaction zone ahead of the luminous flame surface starting at around 50 vol.% and possibly even at lower air admixtures. This evidence of a steep change in refractive index is indicative of a premixed reaction zone involving the added oxygen, which however generates no chemi-ionization and emits no light. Even photographing the flame by radiation emitted at the CH{sup *} and C{sub 2}{sup *} wavelengths shows no sign of its existence. Its burning velocity is about 10 cm/s, when stabilized by the surrounding diffusion flame. The most plausible rationale for these observations is the formation of syngas by the partial oxidation of methane. The subsequent burning of CO and H{sub 2} is known to occur without chemi-ionization or appreciable light emission. (author)

  17. Temperature-dependent ozone chemiluminescence: A new approach for hydrocarbon monitoring

    SciTech Connect

    Marley, N.; Gaffney, J. [Argonne National Lab., IL (United States)

    1996-12-31

    Ozone chemiluminescent reactions have been used for some time to detect oxides of nitrogen, ozone, and olefins in air quality studies. Current procedures use non-methane hydrocarbon analyzers based on the flame ionization detector (FID), which quantitate total non-methane hydrocarbons but do not differentiate between the wide variety of volatile organic classes and oxygenates. The other methodology that has been used, gas chromatography/mass spectroscopy (GC/MS), can measure a variety of individual hydrocarbon species and classes, but it is costly, time-consuming, and labor intensive and is not amenable to real-time measurements. Presented here is preliminary research aimed at the development of an alternative to FID and GC/MS: the ozone chemiluminescent detector (OCD) for measurement of a variety of hydrocarbon species and classes by use of the temperature dependence of ozone chemiluminescent reactions. Responses for various hydrocarbon classes obtained with an OCD operated at 170 C or the FID were compared. The results indicate that the OCD detector responds like a total carbon detector at this temperature, with sensitivities 10-100 times higher than those of a FID. Use of the temperature dependence of the chemiluminescent reaction and prereactors will apparently make a real-time hydrocarbon analyzer based on this approach feasible for determination of high-, moderate-, and low-reactivity hydrocarbon levels in ambient air. The OCD approach may be very useful in determining oxygenate emissions from motor vehicles, particularly alternative fuels. The OCD may also be useful in monitoring of ambient air for natural hydrocarbon emissions.

  18. Electrogenerated chemiluminescence. 58. Ligand-sensitized electrogenerated chemiluminescence in europium labels

    SciTech Connect

    Richter, M.M.; Bard, A.J. [Univ. of Texas, Austin, TX (United States)] [Univ. of Texas, Austin, TX (United States)

    1996-08-01

    The electrochemistry and electrogenerated chemiluminescence (ECL) of a series of europium chelates, cryptates, and mixed-ligand chelate/cryptand complexes were studied. The complexes were of the following general forms: EuL{sub 4}{sup -}, where L = {beta}-diketonate, a bis-chelating ligand (such as dibenzoylmethide), added as salts (A)EuL{sub 4}, where A= tetrabutylammonium ion or piperidinium ion (pipH{sup +}); Eu(crypt){sup 3+}, where crypt = a cryptand ligand, e.g., 4,7,13,16,21-pentaoxa-1,10-diazabicyclo[8,8,5]-tricosa ne; and Eu(crypt)(L){sup 2+} for the mixed-ligand systems. ECL was obtained for the chelates and mixed-ligand systems by reducing the complexes at a Pt electrode in the presence of peroxydisulfate in acetonitrile solutions and was attributed to the electron-transfer reaction between the reduced bound ligands and SO{sub 4}{sup .-}, followed by intramolecular excitation transfer from the excited ligand orbitals to the metal-centered 4f states. No ECL was observed under the same conditions for the europium complexes incorporating only the cryptand ligands in aqueous solution. The ECL spectra matched the photoluminescence spectra with a narrow emission band observed at 612 nm, corresponding to a metal-centered 4f-4f transition. The ECL efficiencies for the ECL-active species were low, about 10{sup -1}-10{sup -4}% of that of the Ru-(bpy){sub 3}{sup 2+}/S{sub 2}O{sub 8}{sup 2-} system under similar conditions. 38 refs., 6 figs., 2 tabs.

  19. Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks

    SciTech Connect

    Lee-Chen, S.F.; Yu, C.T.; Wu, D.R. [Institute of Zoology, Taipei (Taiwan, Province of China)] [and others

    1994-12-31

    When Chinese hamster ovary cells were treated with ultraviolet (UV) light or methyl methane-sulfonate (MMS), a large number of DNA strand breaks could be detected by alkaline elution. These strand breaks gradually disappeared if the treated cells were allowed to recover in a drug-free medium. The presence of nickel or arsenite during the recovery incubation retarded the disappearance of UV-induced strand breaks, whereas the disappearance of MMS-induced strand breaks was retarded by the presence of arsenite or of luminol, a new inhibit for poly(ADP-ribose) synthetase. Luminol, however, had no apparent effect on the repair of UV-induced DNA strand breaks, and nickel had no effect on the repair of MMS-induced DNA strand breaks. When UV- or MMS-treated cells were incubated in cytosine arabinofuranoside (AraC) plus hydroxyurea (HU), a large amount of low molecular weight DNA was detected by alkaline sucrose sedimentation. The molecular weight of these DNAs increased if the cells were further incubated in a drug-free medium. This rejoining of breaks in cells pretreated with UV plus AraC and HU was inhibited by nickel and by arsenite, but not by luminol. The rejoining of breaks in cells pretreated with MMS plus AraC and HU was inhibited by luminol and by arsenite, but not by nickel. These results suggest that different enzymes may be used in DNA resynthesis and/or ligation during the repairing of UV- and MMS-induced DNA strand breaks, and that nickel, luminol, and arsenite may have differential inhibitory effects on these enzymes. 29 refs., 4 figs., 1 tab.

  20. Hot Electron-Induced Electrogenerated Chemiluminescence

    Microsoft Academic Search

    Johanna Suomi; Sakari Kulmala

    \\u000a In this chapter, we discuss the basics of cathodic hot electron-induced electrogenerated chemiluminescence (HECL). In the\\u000a applications of HECL, we discuss, e.g., the usable electrode materials and their advantages as well as the applicable solution\\u000a conditions in aqueous media. We also summarize the luminophore types excitable by this method and their usability as labels\\u000a in practical bioaffinity assay applications.

  1. Native vs photoinduced chemiluminescence in dimethoate determination.

    PubMed

    Catalá-Icardo, M; López-Paz, J L; Choves-Barón, C; Peña-Bádena, A

    2012-01-13

    The determination of dimethoate using either its native chemiluminescent (CL) properties or its photoinduced chemiluminescence obtained by irradiation with a 15 W low-pressure mercury lamp was studied. Thereby, two flow injection systems (FIA) with and without irradiation were exhaustively optimized and their analytical characteristics studied. Better sensitivity and selectivity was found in absence of irradiation, due to the enhancing effect of hexadecylpyridinium chloride (HPC), which acted as a sensitizer. In the developed FIA-CL system, the alkaline hydrolysis of dimethoate with NaOH was performed on-line in presence of HPC. The oxidation of the product of hydrolysis with Ce(IV) in hydrochloric medium induced chemiluminescence. The method provided a limit of detection of only 0.05 ng mL(-1) without any pre-treatment. However, the combination with solid phase extraction allowed the removal of some potential interferents as well as the preconcentration of the pesticide. Finally, the developed method was successfully applied to natural waters with recoveries between 95 and 108%. PMID:22123115

  2. Photoluminescence, chemiluminescence and anodic electrochemiluminescence of hydrazide-modified graphene quantum dots.

    PubMed

    Dong, Yongqiang; Dai, Ruiping; Dong, Tongqing; Chi, Yuwu; Chen, Guonan

    2014-10-01

    Single-layer graphene quantum dots (SGQDs) were refluxed with hydrazine (N2H4) to prepare hydrazide-modified SGQDs (HM-SGQDs). Compared with SGQDs, partial oxygen-containing groups have been removed from HM-SGQDs. At the same time, a lot of hydrazide groups have been introduced into HM-SGQDs. The introduced hydrazide groups provide HM-SGQDs with a new kind of surface state, and give HM-SGQDs unique photoluminescence (PL) properties such as blue-shifted PL emission and a relatively high PL quantum yield. More importantly, the hydrazide-modification made HM-SGQDs have abundant luminol-like units. Accordingly, HM-SGQDs exhibit unique and excellent chemiluminescence (CL) and anodic electrochemiluminescence (ECL). The hydrazide groups of HM-SGQDs can be chemically oxidized by the dissolved oxygen (O2) in alkaline solutions, producing a strong CL signal. The CL intensity is mainly dependent on the pH value and the concentration of O2, implying the potential applications of HM-SGQDs in pH and O2 sensors. The hydrazide groups of HM-SGQDs can also be electrochemically oxidized in alkaline solutions, producing a strong anodic ECL signal. The ECL intensity can be enhanced sensitively by hydrogen peroxide (H2O2). The enhanced ECL intensity is proportional to the concentration of H2O2 in a wide range of 3 ?M to 500 ?M. The detection limit of H2O2 was calculated to be about 0.7 ?M. The results suggest the great potential applications of HM-SGQDs in the sensors of H2O2 and bio-molecules that are able to produce H2O2 in the presence of enzymes. PMID:25132204

  3. Determination of maprotiline in plasma by high-performance liquid chromatography with chemiluminescence detection.

    PubMed

    Ishida, J; Horike, N; Yamaguchi, M

    1995-07-21

    A simple and sensitive high-performance liquid chromatographic method is described for the determination of maprotiline, an antidepressant, in plasma. After a single-step extraction from plasma (100 microliters) with n-hexaneisoamylalcohol (19:1, v/v), the drug and desipramine (internal standard) are converted into their chemiluminescent derivatives by reaction with 6-isothiocyanatobenzo[g]phthalazine-1,4(2H,3H)-dione, a new chemiluminescence derivatization reagent for amines. The derivatives are separated within 60 min on a reversed-phase column, TSKgel ODS-80, using isocratic elution with acetonitrile-100 mM acetate buffer (pH 3.2), and produced chemiluminescence by reaction with hydrogen peroxide in the presence of potassium hexacyanoferrate(III) in alkaline medium. The detection limit for maprotiline added to plasma is 0.36 pmol (0.1 ng)/ml plasma (1.5 fmol on column), at a signal-to-noise ratio of 3. PMID:7581917

  4. A molecularly imprinted polymer based a lab-on-paper chemiluminescence device for the detection of dichlorvos.

    PubMed

    Liu, Wei; Guo, Yumei; Luo, Jing; Kou, Juan; Zheng, Hongyan; Li, Baoxin; Zhang, Zhujun

    2015-04-15

    In this work, a new molecularly imprinted polymer (MIP) based lab-on-paper device with chemiluminescence (CL) detection of dichlorvos (DDV) was designed. With the circle-shaped device, the MIP layer with certain depth was synthesized and adsorbed on the paper surface and DDV can be selectively imprinted on it. The adsorption and washing procedures can be achieved well on the paper-based chip. The paper-based device was fabricated by a simple cutting method and many chips can be made at the same time. On the basis of DDV enhancing CL of luminol-H2O2 greatly, the proposed MIP based lab-on-paper CL device showed better selectivity to DDV and it has been applied to the determination of DDV in vegetables in the range of 3.0ng/mL-1.0?g/mL with the detection limit of 0.8ng/mL. This study has made a successful attempt in the development of highly selective and sensitive monitoring of DDV in real samples and will provide a new approach for sensitive and specific assay in environmental monitoring. PMID:25659812

  5. Assessment of Antioxidant Activity of Spray Dried Extracts of Psidium guajava Leaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils

    PubMed Central

    Fernandes, M. R. V.; Azzolini, A. E. C. S.; Martinez, M. L. L.; Souza, C. R. F.; Lucisano-Valim, Y. M.; Oliveira, W. P.

    2014-01-01

    This work evaluated the physicochemical properties and antioxidant activity of spray dried extracts (SDE) from Psidium guajava L. leaves. Different drying carriers, namely, maltodextrin, colloidal silicon dioxide, Arabic gum, and ?-cyclodextrin at concentrations of 40 and 80% relative to solids content, were added to drying composition. SDE were characterized through determination of the total phenolic, tannins, and flavonoid content. Antioxidant potential of the SDE was assessed by two assays: cellular test that measures the luminol-enhanced chemiluminescence (LumCL) produced by neutrophils stimulated with phorbol myristate acetate (PMA) and the DPPH radical scavenging (DPPH? method). In both assays the antioxidant activity of the SDE occurred in a concentration-dependent manner and showed no toxicity to the cells. Using the CLlum method, the IC50 ranged from 5.42 to 6.50?µg/mL. The IC50 of the SDE ranged from 7.96 to 8.11?µg/mL using the DPPH• method. Psidium guajava SDE presented significant antioxidant activity; thus they show high potential as an active phytopharmaceutical ingredient. Our findings in human neutrophils are pharmacologically relevant since they indicate that P. guajava SDE is a potential antioxidant and anti-inflammatory agent in human cells. PMID:24822200

  6. In vitro inhibitory effects of Moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes.

    PubMed

    Vongsak, Boonyadist; Gritsanapan, Wandee; Wongkrajang, Yuvadee; Jantan, Ibrahim

    2013-11-01

    The ethanol extract of Moringa oleifera Lam. leaves and its major constituents, crypto-chlorogenic acid, quercetin 3-O-glucoside and kaempferol 3-O-glucoside, were investigated on the respiratory burst of human whole blood and isolated human polymorphonuclear leukocytes (PMNs) using a luminol-based chemiluminescence assay. The chemotactic migration of PMNs was also investigated using the Boyden chamber technique. The ethanol extract demonstrated inhibitory activities on the oxidative burst and the chemotactic migration of PMNs. Quercetin 3-O-glucoside, crypto-chlorogenic acid, and kaempferol 3-O-glucoside, isolated from the extract, expressed relatively strong inhibitory activity on the oxidative burst of PMNs with IC50 values of 4.1, 6.7 and 7.0 microM, respectively, comparable with that of aspirin. They also demonstrated strong inhibition of chemotatic migration of PMNs with IC50 values of 9.5, 15.9 and 18.2 microM, respectively. The results suggest that M. oleifera leaves could modulate the immune response of human phagocytes, linking to its ethnopharmacological use as an anti-inflammatory agent. The immunomodulating activity of the plant was mainly due to its major components. PMID:24427941

  7. Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence.

    PubMed Central

    Ullman, E F; Kirakossian, H; Singh, S; Wu, Z P; Irvin, B R; Pease, J S; Switchenko, A C; Irvine, J D; Dafforn, A; Skold, C N

    1994-01-01

    A method for monitoring formation of latex particle pairs by chemiluminescence is described. Molecular oxygen is excited by a photosensitizer and an antenna dye that are dissolved in one of the particles. 1 delta gO2 diffuses to the second particle and initiates a high quantum yield chemiluminescent reaction of an olefin that is dissolved in it. The efficiency of 1 delta gO2 transfer between particles is approximately 3.5%. The technique permits real-time measurement of particle binding kinetics. Second-order rate constants increase with the number of receptor binding sites on the particles and approach diffusion control. By using antibody-coated particles, a homogeneous immunoassay capable of detecting approximately 4 amol of thyroid-stimulating hormone in 12 min was demonstrated. Single molecules of analyte produce particle heterodimers that are detected even when no larger aggregates are formed. PMID:8202502

  8. Continuous flow determination of residual aqueous ozone with membrane seapartion-chemiluminescent detection

    SciTech Connect

    Aoki, T.

    1988-01-01

    A method for the determination of ultra-trace aqueous ozone utilizing a membrane-separation process and a chemiluminescent detector is proposed. The microporous PTFE tube was used as separator to transfer aqueous ozone into a gas phase. Air bubbling was used to enhance the separation. Chemiluminescent signals produced from the reaction of ozone with ethylene in air were linearly proportional to concentration of aqueous ozone from 8 ppb to 9.5 ppm. The relative standard deviation (n=4) was 3.5% at 0.2 ppm. The time it took from starting the sample flow until the signal to reach a stable level was 1.5 min. The interference from chromium(VI), manganese(VII), chlorine, bromine, phthalate, and sulfophthalate was completely eliminated.

  9. 4-Dimethylaminobenzylamine as a sensitive chemiluminescence derivatization reagent for 5-hydroxyindoles and its application to their quantification in human platelet-poor plasma.

    PubMed

    Ishida, J; Takada, M; Hitoshi, N; Iizuka, R; Yamaguchi, M

    2000-02-11

    A selective and sensitive high-performance liquid chromatographic method with chemiluminescence detection for the determination of 5-hydroxyindoles is described, based on the reaction of 5-hydroxyindoles with 4-dimethylaminobenzylamine. Serotonin, 5-hydroxyindole-3-acetic acid, 5-hydroxytryptophol, 5-hydroxyindole-3-acetamide and N-acetyl-5-hydroxytryptamine were used as model compounds to optimize the derivatization and chemiluminescent reaction. The reagent reacts with 5-hydroxyindoles in slightly alkaline media in the presence of potassium hexacyanoferrate(III) to give the corresponding derivatives, which can be separated on a reversed-phase column, Wakosil-II 5C18RS, with aqueous acetonitrile as an eluent. The derivatives were detected by peroxyoxalate chemiluminescence detection. The detection limits are in the range of 0.5-1.2 fmol per 100-microl injection. The method was applied to the simultaneous determination of serotonin and 5-hydroxyindole-3-acetic acid in human platelet-poor plasma. PMID:10718637

  10. Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry

    SciTech Connect

    Powe, Aleeta [University of Louisville, Louisville; Das, Susmita [Louisiana State University; Lowry, Mark [Lousianna State University; El-Zahab, Bilal [Lousianna State University; Fakayode, Sayo [Winston-Salem State University; Geng, Maxwell [University of Iowa; Baker, Gary A [ORNL; Wang, Lin [Southern Illinois University; McCarroll, Matthew [Southern Illinois University; Patonay, Gabor [Georgia State University, Atlanta; Li, Min [Lousianna State University; Aljarrah, Mohannad [Louisiana State University; Neal, Sharon [University of Delaware; Warner, Isiah M [ORNL

    2010-01-01

    This review covers the 2 year period since our last review (1) from January 2008 through December 2009. A computer search of Chemical Abstracts provided most of the references for this review. A search for documents written in English containing the terms fluorescence or phosphorescence or chemiluminescence published in 2008-2009 resulted in more than 100 000 hits. An initial screening reduced this number to approximately 23 000 publications that were considered for inclusion in this review. Key word searches of this subset provided subtopics of manageable size. Other citations were found through individual searches by the various authors who wrote a particular section of this review.

  11. Chemiluminescence of BO{sub 2} to map the creation of thermal NO in flames

    SciTech Connect

    Maligne, D. [CORIA CNRS UMR6614, Site Universitaire du Madrillet, BP12, 76801 St Etienne du Rouvray Cedex (France)]|[Renault s.a.s., 1 allee Cornuel, 91510 Lardy (France)]|[IFP, 1 et 4 avenue de Bois-Preau, 92852 Rueil-Malmaison Cedex (France); Cessou, A.; Stepowski, D. [CORIA CNRS UMR6614, Site Universitaire du Madrillet, BP12, 76801 St Etienne du Rouvray Cedex (France)

    2009-02-15

    The aim of this study is to detect and map the local conditions that generate thermal NO in flames. According to the Zeldovich mechanism, the formation of NO comes from the local conjunction of a high concentration of atomic oxygen and a temperature above a critical high level imposed by the high activation energy of the rate-limiting reaction. The green light emitted when a flame is seeded with boron salts is a chemiluminescence from the BO{sup *}{sub 2} that is chemically formed in its excited state when BO reacts with atomic oxygen. As the rate of this oxidation is also strongly increasing with temperature, the chemiluminescence of BO{sub 2} depends on the concentration of atomic oxygen and on the temperature in a way similar to the formation rate of thermal NO. This double analogy suggests the possibility of an experimental in situ simulation of the formation rate of thermal NO or at least the use of the chemiluminescence of BO{sub 2} to map the sites where thermal NO is being created. Spectroscopic experiments and comparisons with numerical simulations have been performed to test the feasibility of this technique in laminar premixed and diffusion methane/air flames. The agreement is good except in the burnt gases of fuel-rich flames. Imaging strategies with different spectral filters have been developed in the same flames to overcome the problem of interference from soot radiation in diffusion flames. (author)

  12. Forensic Luminol Blood Test for Preventing Cross-contamination in Dentistry: An Evaluation of a Dental School Clinic

    PubMed Central

    Bortoluzzi, Marcelo Carlos; Cadore, Peterson; Gallon, Andrea; Imanishi, Soraia Almeida Watanabe

    2014-01-01

    Background: More than 200 different diseases may be transmitted from exposure to blood in the dental setting. The aim of this study is to identify possible faults in the crosscontamination chain control in a dental school clinic searching for traces of blood in the clinical contact surfaces (CCS) through forensic luminol blood test. Methods: Traces of invisible blood where randomly searched in CCS of one dental school clinic. Results: Forty eight surfaces areas in the CCS were tested and the presence of invisible and remnant blood was identified in 28 (58.3%) items. Conclusions: We suggest that the luminol method is suitable for identifying contamination with invisible blood traces and this method may be a useful tool to prevent cross-contamination in the dental care setting. PMID:25400895

  13. Bilirubin chemiluminescence induced by the attack of active oxygen species.

    PubMed

    Watanabe, H; Nagoshi, T; Agatsuma, S; Kobayashi, M; Inaba, H

    1992-01-01

    Ultraweak chemiluminescence (CL) from bilirubin occurs in the presence of triplet oxygen and is stimulated by the addition of aldehydes. Active oxygen species also enhance bilirubin CL, in the absence of aldehydes. An inhibitory effect of active oxygen scavengers on the CL indicated that active oxygens generated from the decomposition of added hydrogen peroxide or from the xanthine-xanthine oxidase reaction contributed to the CL from bilirubin molecules. However, the contribution of singlet oxygen to the CL disappeared in the presence of formaldehyde. This suggested that the scission of tetrapyrrole bonds via a dioxetane intermediate or the production of triplet carbonyls from the oxidation of aldehydes by singlet oxygen was not involved in the CL, at least in the presence of formaldehyde. The spectrum of CL induced by the generation of active oxygen was the same as that from the aldehyde-enhanced CL reaction. We propose that the formation of a hydroperoxide (and/or hydroxide) bilirubin intermediate, but not a dioxetane, may be involved in the excitation of bilirubin molecules for CL. PMID:1322633

  14. Measurement and simulation of rotationally-resolved chemiluminescence spectra in flames

    NASA Astrophysics Data System (ADS)

    Brockhinke, A.; Krüger, J.; Heusing, M.; Letzgus, M.

    2012-06-01

    In recent years, there has been renewed interest in chemiluminescence, since it has been shown that these emissions can be used to determine flame parameters such as stoichiometry and heat release under some conditions. Even though the origin of these emissions has been known for a long time, little attention has been paid to the detailed analysis of the spectral structure. In this contribution, we present rotationally-resolved spectra of all important chemiluminescent emissions OH A-X, CH B-X, CH A-X, and C2 d-a in CH4/air flames. A numerical model based on the LASKIN ? 2 code has been developed that allows, for the first time, to accurately predict the shape of the measured spectra for all of these transitions. Reabsorption of chemiluminescence within the emitting flame is shown to be a major factor, affecting both intensity and structure of OH? spectra. Even in lab-scale flames, it might change the intensity of individual lines by a factor of 5. The shape of chemiluminescence spectra depends on several processes including initial state distribution and rotational and vibrational energy transfer (which, in turn, depend on the collisional environment and the temperature). It is shown that chemical reactions form OH? in highly excited states and that the number of collisions is not sufficient to equilibrate the initial distribution. Therefore, high apparent temperatures are necessary to describe the shape of the measured spectra. In contrast, CH? is formed with less excess energy and the spectral shape is very close to thermal. The rotational structure of C2^{*} is close to thermal equilibrium as well. Vibrational temperatures are, however, significantly higher than the flame temperature. Implications and perspectives for flame measurements are discussed.

  15. Imaging of DNA sequences with chemiluminescence.

    PubMed Central

    Tizard, R; Cate, R L; Ramachandran, K L; Wysk, M; Voyta, J C; Murphy, O J; Bronstein, I

    1990-01-01

    We have coupled a chemiluminescent detection method that uses an alkaline phosphatase label to the genomic DNA sequencing protocol of Church and Gilbert [Church, G. M. & Gilbert, W. (1984) Proc. Natl. Acad. Sci. USA 81, 1991-1995]. Images of sequence ladders are obtained on x-ray film with exposure times of less than 30 min, as compared to 40 h required for a similar exposure with a 32P-labeled oligomer. Chemically cleaved DNA from a sequencing gel is transferred to a nylon membrane, and specific sequence ladders are selected by hybridization to DNA oligonucleotides labeled with alkaline phosphatase or with biotin, leading directly or indirectly to deposition of enzyme. If a biotinylated probe is used, an incubation with avidin-alkaline phosphatase conjugate follows. The membrane is soaked in the chemiluminescent substrate (AMPPD) and is exposed to film. Dephosphorylation of AMPPD leads in a two-step pathway to a highly localized emission of visible light. The demonstrated shorter exposure times may improve the efficiency of a serial reprobing strategy such as the multiplex sequencing approach of Church and Kieffer-Higgins [Church, G. M. & Kieffer-Higgins, S. (1988) Science 240, 185-188]. Images PMID:2191292

  16. Electrogenerated chemiluminescence 73: acid–base properties, electrochemistry, and electrogenerated chemiluminescence of neutral red in acetonitrile

    Microsoft Academic Search

    Jai-Pil Choi; Allen J. Bard

    2004-01-01

    We characterized the acid–base properties, electrochemistry, and electrogenerated chemiluminescence (ECL) of neutral red in acetonitrile (MeCN). To determine the acid–base properties, the basic form (NR) of neutral red was prepared and titrated with anhydrous HClO4, yielding pKa=6.5 in MeCN. NR showed three irreversible electrochemical oxidations and a one-electron reversible reduction. However, the acid form (NRH+) was reduced at more positive

  17. Chemiluminescence response of human natural killer cells. I. The relationship between target cell binding, chemiluminescence, and cytolysis

    PubMed Central

    1982-01-01

    The binding of tumor cells or fetal fibroblasts to human natural killer (NK) cells led to a rapid chemiluminescence response within seconds of target-effector interaction. The degree of chemiluminescence was dependent on the concentration of NK-enriched lymphocytes or target cells, and plasma membrane vesicles from K562 also induced a chemiluminescence response. Mild glutaraldehyde treatment of effector cells abrogated their ability to generate chemiluminescence, whereas K562 target cells treated in the same way were almost fully able to induce a chemiluminescence response to NK-enriched lymphocytes. These results show a directionality of response with NK as the responders and tumor cells as the stimulators. A survey of eight different tumor cell lines and fetal fibroblast lines revealed a striking correlation (r greater than 0.93, P less than 0.001) between the ability of a given line to bind to NK-enriched lymphocytes, induce chemiluminescence, and to be lysed. Three differentiated sublines of K562 grown in butyrate and cloned induced little chemiluminescence compared with the K562 parent, and they were selectively resistant to NK-mediated binding and cytolysis. In addition, treatment of K562 cells with higher concentrations of glutaraldehyde for longer periods led to varying degrees of target antigen preservation, as measured in cold target competition assays and in conjugate formation. The degree of NK target antigen preservation correlated directly with the ability of the cells to induce chemiluminescence (r greater than 0.95). The degree of NK activation was also important because interferon-pretreated effectors generated more chemiluminescence upon stimulation with K562 or MeWo targets. Monocytes or granulocytes did not contribute to the chemiluminescence induced by NK-sensitive targets. Some NK-resistant tumor cell lines were sensitive to monocyte-mediated cytolysis and also induced chemiluminescence in monocytes but not NK cells. These results show that the target structures recognized by the NK cell may play a role in NK activation because the degree of chemiluminescence was directly proportional to the ability of a given target cell line to bind to the NK cell and to be lysed. PMID:6178787

  18. 4-(6,7-Dihydro-5,8-dioxothiazolo[4,5-g]phthalazin-2-yl)benzoic acid N-hydroxysuccinimide ester as a highly sensitive chemiluminescence derivatization reagent for amines in liquid chromatography.

    PubMed

    Yoshida, H; Nakao, R; Matsuo, T; Nohta, H; Yamaguchi, M

    2001-01-12

    4-(6,7-Dihydro-5,8-dioxothiazolo[4,5-g]phthalazin-2-yl)benzoic acid N-hydroxysuccinimide ester was synthesized as a highly sensitive and selective chemiluminescence derivatization reagent for primary and secondary amines in liquid chromatography. Methyl-n-octylamine, n-nonylamine and n-decylamine were used as model compounds to optimize the derivatization, separation and chemiluminescence reaction conditions. This reagent reacts selectively with amines in the presence of triethylamine to give the highly chemiluminescent derivatives, which produce chemiluminescence by reaction with hydrogen peroxide in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The chemiluminescent derivatives of the three amines can be separated within 20 min by reversed-phase liquid chromatography with isocratic elution, followed by chemiluminescence detection. The detection limits (signal-to-noise ratio=3) for primary and secondary amines are at sub-fmol levels for a 20-microl injection. Furthermore, this method was applicable to the determination of amantadine in human plasma. PMID:11217046

  19. Flow injection chemiluminescence determination of naphazoline hydrochloride in pharmaceuticals.

    PubMed

    Iranifam, Mortaza; Sorouraddin, Mohammad H

    2014-02-01

    A simple and sensitive flow injection chemiluminescence (FI-CL) method was developed for the determination of naphazoline hydrochloride (NPZ). The method is based on the enhancing effect of NPZ on the weak CL signal from the reaction of KIO4 with H2 O2 . Experimental parameters that affected the CL signal, including the pH of the KIO4 solution, concentrations of KIO4 , H2 O2 and disodium-EDTA and flow rate were optimized. Under the optimum conditions, the increment of CL intensity was linearly proportional to the concentration of NPZ in the range 5.0 × 10(-6) to 70 × 10(-6) mol/L. The detection limit was 1.0 × 10(-6) mol/L and the relative standard deviation for 50 × 10(-6) mol/L NPZ solution was 2.8% (n = 11). In addition, a high throughput of 120 samples/h was achieved. The utility of this method was demonstrated by determining NPZ in pharmaceuticals. PMID:23463582

  20. Measurement of chemiluminescence in freshly drawn human blood

    Microsoft Academic Search

    T. Kato; H. Wokalek; E. Schöpf; H. Eggert; M. Ernst; E. Th. Rietschel; H. Fischer

    1981-01-01

    Summary The present investigations were undertaken to find out whether chemiluminescence measurements of stimulated granulocytes can be carried out in freshly drawn blood and — because of the ease of the method — be introduced into routine diagnostics.

  1. Need total sulfur content? Use chemiluminescence

    SciTech Connect

    Kubala, S.W.; Campbell, D.N. [Fluid Data, Inc., Angleton, TX (United States); DiSanzo, F.P. [Mobil Technology Co., Paulsboro, NJ (United States)

    1996-09-01

    Regulations issued by the United States Environmental Protection Agency require petroleum refineries to reduce or control the amount of total sulfur present in their refined products. These legislative requirements have led many refineries to search for online instrumentation that can produce accurate and repeatable total sulfur measurements within allowed levels. Several analytical methods currently exist to measure total sulfur content. They include X-ray fluorescence (XRF), microcoulometry, lead acetate tape, and pyrofluorescence techniques. Sulfur-specific chemiluminescence detection (SSCD) has recently received much attention due to its linearity, selectivity, sensitivity, and equimolar response. However, its use has been largely confined to the area of gas chromatography. This article focuses on the special design considerations and analytical utility of an SSCD system developed to determine total sulfur content in gasoline. The system exhibits excellent linearity and selectivity, the ability to detect low minimum levels, and an equimolar response to various sulfur compounds. 2 figs., 2 tabs.

  2. Water-binding phospholipid nanodomains and phase-separated diacylglycerol nanodomains regulate enzyme reactions in lipid monolayers.

    PubMed

    Nagashima, Teruyoshi; Uematsu, Shogo

    2015-02-01

    Phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) nanodomains covered with bound water as well as diacylglycerol 1-palmitoyl-2-oleoyl-sn-glycerol (POG) nanodomains separated from a lipid membrane were studied, using monolayer surfaces of POPC hydrolyzed by phospholipase C (PLC). The investigation was based on the analysis of compression isotherms and on atomic force microscope (AFM) observations of Langmuir-Blodgett (LB) films and Langmuir-Schaefer (LS) films. The results included reaction rate constants obtained by kinetic analysis of phosphocholine at surface pressures from 0.1 to 31 mN/m and determined by a luminol-enhanced chemiluminescence method. Monolayer elastic modulus values and fluorescence microscopic images confirmed that hydrolysis by PLC progressed in the intermediate monolayer between a liquid-expanded (L1) film and a liquid-condensed (L2) film at 2-17 mN/m. Furthermore, the intermediate film was confirmed to consist of L1 film and the POPC nanodomains in the L2 state are covered with bound water, conclusions based on the following AFM results: (1) nanodomains in POPC LS films were catalyzed by PLC, (2) POG nanodomains extended out from LB films of mixed POPC/POG 9/1 (mol/mol) monolayers, and (3) POPC LS films were covered with bound water, as indicated by cross-sectional analysis. At the optimal surface pressure of 10 mN/m, when POPC nanodomains (L2), with internal diameters of ?75 nm, were hydrolyzed by PLC, they shrank down into pockets of the same size as those that appeared with POG. The resulting pocket sizes on LS films were in agreement with POG nanodomain sizes on LB films. This study demonstrated that PLC reacted with POPC nanodomains (L2) dispersed in L1/L2 mixed phase monolayers selectively and that POG nanodomains were phase-separated from the monolayer as hydrolysis proceeded. PMID:25565217

  3. Enzyme-linked immunosorbent assay by enhanced chemiluminescence detection for the standardization of estrogenic miroestrol in Pueraria candollei Graham ex Benth.

    PubMed

    Yusakul, Gorawit; Udomsin, Orapin; Tanaka, Hiroyuki; Morimoto, Satoshi; Juengwatanatrakul, Thaweesak; Putalun, Waraporn

    2014-11-01

    Miroestrol (ME) is a potent phytoestrogen from the P. candollei tuberous root. It has been approved for use in clinical trials due to its beneficial effect on disorders associated with estrogen deficiency. To ensure medical efficacy and safety, high performance analytical methods for ME analysis are required to standardize products from the P. candollei root. An enhanced chemiluminescence enzyme-linked immunosorbent assay (ECL-ELISA) was developed and validated using a polyclonal antibody against ME and a chemiluminescent system of luminol-H2 O2 -horseradish peroxidase-4-(1-imidazolyl) phenol. The ECL-ELISA system exhibited linearity over a concentration range of 0.31-10.00?ng?mL(-1) , for which the relative standard variation (%RSD) was less than 10% for both intra- and interplate determinations. The ECL-ELISA is reliable for the determination of ME as reflected by the high recovery percentage (101.22-103.06%). As a comparative analysis, the ME content in each sample determined by ECL-ELISA was correlated with high coefficients of determination with colorimetric ELISA (R(2) ?=?0.998) and high performance liquid chromatography (HPLC) (R(2) ?=?0.998) methods. The ECL-ELISA method could be applied to all of the commercial products containing P. candollei root, when the products contain between 0.706?±?0.046 and 13.123?±?0.794?µg?g(-1) dry wt. of ME. This method is useful as a high performance analytical method for the quantity control of ME in raw materials and end products at both the research and industrial levels. Copyright © 2014 John Wiley & Sons, Ltd. PMID:25363375

  4. Aqueous nitrite ion determination by selective reduction and gas phase nitric oxide chemiluminescence

    NASA Technical Reports Server (NTRS)

    Dunham, A. J.; Barkley, R. M.; Sievers, R. E.; Clarkson, T. W. (Principal Investigator)

    1995-01-01

    An improved method of flow injection analysis for aqueous nitrite ion exploits the sensitivity and selectivity of the nitric oxide (NO) chemilluminescence detector. Trace analysis of nitrite ion in a small sample (5-160 microL) is accomplished by conversion of nitrite ion to NO by aqueous iodide in acid. The resulting NO is transported to the gas phase through a semipermeable membrane and subsequently detected by monitoring the photoemission of the reaction between NO and ozone (O3). Chemiluminescence detection is selective for measurement of NO, and, since the detection occurs in the gas-phase, neither sample coloration nor turbidity interfere. The detection limit for a 100-microL sample is 0.04 ppb of nitrite ion. The precision at the 10 ppb level is 2% relative standard deviation, and 60-180 samples can be analyzed per hour. Samples of human saliva and food extracts were analyzed; the results from a standard colorimetric measurement are compared with those from the new chemiluminescence method in order to further validate the latter method. A high degree of selectivity is obtained due to the three discriminating steps in the process: (1) the nitrite ion to NO conversion conditions are virtually specific for nitrite ion, (2) only volatile products of the conversion will be swept to the gas phase (avoiding turbidity or color in spectrophotometric methods), and (3) the NO chemiluminescence detector selectively detects the emission from the NO + O3 reaction. The method is free of interferences, offers detection limits of low parts per billion of nitrite ion, and allows the analysis of up to 180 microL-sized samples per hour, with little sample preparation and no chromatographic separation. Much smaller samples can be analyzed by this method than in previously reported batch analysis methods, which typically require 5 mL or more of sample and often need chromatographic separations as well.

  5. Hairpin assembly-triggered cyclic activation of a DNA machine for label-free and ultrasensitive chemiluminescence detection of DNA.

    PubMed

    Chen, Jia; Qiu, Hongdeng; Zhang, Mingliang; Gu, Tongnian; Shao, Shijun; Huang, Yong; Zhao, Shulin

    2015-06-15

    DNA plays important regulatory roles in many life activities. Here, we have developed a novel label-free, ultrasensitive and specific chemiluminescence (CL) assay protocol for DNA detection based on hairpin assembly-triggered cyclic activation of a DNA machine. The system involves two hairpin structures, H1 and H2. Firstly, a target DNA binds with and opens the hairpin structure of H1. Then, H2 hybridizes with H1 and displaces the target DNA, which is used to trigger another new hybridization cycle between H1 and H2, leading to the generation of numerous H1-H2 complexes. The generated H1-H2 complexes are further activated with the help of polymerase and nicking enzyme, continuously yielding a large amount of G-riched DNA fragments. The G-riched DNA fragment products interact with hemin to form the activated HRP-mimicking DNAzymes that can catalyze the oxidation of luminol by H2O2 to produce strong CL signal resulting in an amplified sensing process. Our newly proposed homogeneous assay enables the quantitative measurement of p53 DNA (as a model) with a detection limit of 0.85fM, which is at least 5 orders of magnitude lower than that of traditional unamplified homogeneous optical approaches. Moreover, this assay exhibits high discrimination ability even against a single base mismatch. In addition, this strategy is also capable of detecting p53 DNA in complex biological samples. The proposed sensing approach might hold a great promise for further applications in biomedical research and early clinical diagnosis. PMID:25638797

  6. Highly sensitive chemiluminescence immunoassay on chitosan membrane modified paper platform using TiO2 nanoparticles/multiwalled carbon nanotubes as label.

    PubMed

    Li, Weiping; Ge, Shenguang; Wang, Shoumei; Yan, Mei; Ge, Lei; Yu, Jinghua

    2013-01-01

    A highly sensitive chemiluminescence (CL) immunoassay was incorporated into a low-cost microfluidic paper-based analytical device (?-PAD) to fabricate a facile paper-based CL immunodevice (denoted as ?-PCLI). This ?-PCLI was constructed by covalently immobilizing capture antibody on a chitosan membrane modified ?-PADs, which was developed by simple wax printing methodology. TiO2 nanoparticles coated multiwalled carbon nanotubes (TiO2/MWCNTs) were synthesized as an amplification catalyst tag to label signal antibody (Ab2). After sandwich-type immunoreactions, the TiO2/MWCNTs were captured on the surface of ?-PADs to catalyze the luminol-p-iodophenol-H2O2 CL system, which produced an enhanced CL emission. Using prostate-specific antigen as a model analyte, the approach provided a good linear response range from 0.001 to 20 ng/mL with a low detection limit of 0.8 pg/mL under optimal conditions. This ?-PCLI showed good reproducibility, selectivity and stability. The assay results of prostate-specific antigen in clinical serum samples were in good agreement with that obtained by commercially used electrochemiluminescence methods at the Cancer Research Center of Shandong Tumor Hospital (Jinan, Shandong Province, China). This ?-PCLI could be very useful to realize highly sensitive, qualitative point-of-care testing in developing or developed countries. PMID:23355319

  7. A new strategy for the detection of adenosine triphosphate by aptamer/quantum dot biosensor based on chemiluminescence resonance energy transfer.

    PubMed

    Zhou, Zi-Ming; Yu, Yong; Zhao, Yuan-Di

    2012-09-21

    We designed an aptasensor for the detection of adenosine triphosphate (ATP) based on chemiluminescence resonance energy transfer (CRET). An adenosine aptamer was cut into two pieces of ssDNA, which were attached to quantum dots (QDs) and horse radish peroxidase (HRP), respectively. They could reassemble into specific structures in the presence of ATP and then decrease the distance of HRP and QDs. ATP detection can be easily realized according to the fluorescent intensity of QDs, which is excited by CRET between luminol and QDs. Results show that the concentration of ATP is linear relation with the fluorescent intensity of the peak of QDs emission and the linear range for the linear equation is from 50 ?M to 231 ?M and the detection limit was 185 nM. When the concentration of ATP was 2 mM, the efficiency of CRET is 13.6%. Good specificity for ATP had been demonstrated compared to thymidine triphosphate (TTP), cytidine triphosphate (CTP) and guanosine triphosphate (GTP), when 1 mM of each was added, respectively. This method needs no external light source and can avoid autofluorescence and photobleaching, and ATP can be detected selectively, specifically, and sensitively in a low micromolar range, which means that the strategy reported here can be applicable to the detection of several other target molecules. PMID:22832507

  8. Ultratrace determination of silver, gold, and iron oxide nanoparticles by micelle mediated preconcentration/selective back-extraction coupled with flow injection chemiluminescence detection.

    PubMed

    Tsogas, George Z; Giokas, Dimosthenis L; Vlessidis, Athanasios G

    2014-04-01

    A new method has been developed for the ultrasensitive determination of silver, gold, and iron oxide nanoparticles in environmental samples. Cloud point extraction was optimized and used as a means to extract and preconcentrate all nanoparticle species simultaneously from the same sample. The extracted nanoparticles were sequentially isolated from the surfactant-rich phase by a new selective back-extraction procedure and dissociated into their precursor metal ions. Each ion solution was injected in a flow injection analysis (FIA) manifold, accommodating the chemiluminogenic oxidation of luminol, in order to amplify chemiluminescence (CL) emission in a manner proportional to its concentration. Under the optimum experimental conditions, the detection limits were brought down to the picomolar and femtomolar concentration levels with satisfactory analytical features in terms of precision (2.0-13.0%), selectivity against dissolved ions, and recoveries (74-114%). The method was successfully applied to the determination of iron oxide, silver, and gold nanoparticles in environmental samples of different complexity, ranging from unpolluted river water to raw sewage. The developed method could also serve as a basis for future deployment of molecular spectrometry detectors for the selective determination and speciation analysis of nanoparticles in environmental applications. PMID:24576255

  9. Application of horse-radish peroxidase linked chemiluminescence to determine the production mechanism of Shiga-like toxins by E. coli O157:H7

    NASA Astrophysics Data System (ADS)

    Tu, Shu-I.; Uknalis, Joseph; Gehring, Andrew; He, Yiping

    2007-09-01

    A sandwiched immunoassay consisting of toxin capture by immunomagnetic beads (IMB) and toxin detection by horseradish peroxidase (HRP) linked chemiluminescence was used to follow the production of Shiga-like toxins (SLT) by E. coli O157:H7. The intensity of luminescence generated by the oxidation of luminol-liked compounds was used to represent the concentration of toxins produced. The time-course of SLT production by E. coli O157:H7 under different conditions was investigated. In pure culture, optimal generation of SLT showed a significant delay than the steady state of cell growth. In mixed cultures of SLT producing E. coli O157:H7 and non-SLT producing E. coli K-12 strain, the production of toxins was substantially decreased. However, the growth of E. coli O157:H7 was not affected by the presence of K-12 strain. This decrease in SLT production was also observed in radiation-sterile ground beef. In regular ground beef that might contain numerous other bacteria, the growth of E. coli O157:H7 in EC media was not significantly affected but the lowered production of SLT was observed. The results showed that mechanism of inducing SLT production was complex with both the growth time and growth environment could influence SLT production. The addition of homo-serine lactone to the growth media enhanced the production of SLT. Thus, possibly cell-cell communication may have a role in SLT production by E. coli O157:H7.

  10. An introduction to chemiluminescence methods for lubricant oxidation studies. Interim report 19 Mar 81-31 Mar 82

    SciTech Connect

    Clark, D.B.; Weeks, S.J.; Hsu, S.M.

    1982-04-01

    An introductory review of chemiluminescence (CL) techniques describes applications for the study of oxidation of fuels and lubricants. Reviews of chemiluminescence and oxidation are briefly discussed. The mechanism of CL as it applies to complex hydrocarbon systems is discussed. Several steady state and nonsteady state kinetic methods are discussed with respect to the ability of each technique to give useful information about the kinetics and mechanisms of oxidation. Applications of CL techniques for fuels and lubricants, as well as simple hydrocarbon systems, are discussed. The unique properties of CL methods for materials testing (e.g. lubricant oxidation stability) as well as fundamental understanding of chemical oxidation reactions are emphasized. Studies which evaluate petroleum and synthoil products, oxidation inhibitors and metal catalysts are discussed. Instrumentation capabilities are reviewed and critical features of instrumental design are discussed.

  11. Rapid determination of Papaver somniferum alkaloids in process streams using monolithic column high-performance liquid chromatography with chemiluminescence detection.

    PubMed

    Costin, Jason W; Lewis, Simon W; Purcell, Stuart D; Waddell, Lucy R; Francis, Paul S; Barnett, Neil W

    2007-07-30

    We have combined high-performance liquid chromatography (HPLC) separations using a monolithic column with acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II) chemiluminescence detection in a rapid and highly sensitive method to monitor the process of extracting opiate alkaloids from Papaver somniferum. Due to the high flow rates allowed with the monolithic column and the inherent selectivity of the chemiluminescence reactions, the four predominant alkaloids--morphine, codeine, oripavine and thebaine--were determined in less than 2 min. The results obtained with numerous process samples compared favourable with those of the standard HPLC methodology. Limits of detection were 1x10(-10) M, 5x10(-10) M, 5x10(-10) M and 1x10(-9) M, for morphine, codeine, oripavine and thebaine, respectively. PMID:17658308

  12. reactions

    Microsoft Academic Search

    S. Reinker; R. M. Altman; J. Timmer

    Gene regulatory, signal transduction and metabolic networks are major areas of interest in the newly emerging field of systems biology. In living cells, stochastic dynamics play an important role; however, the kinetic parameters of biochemical reactions necessary for modelling these processes are often not accessible directly through experiments. The problem of estimating stochastic reaction constants from molecule count data measured,

  13. Chemiluminescence assay for oxidatively modified myoglobin.

    PubMed

    Vuletich, J L; Osawa, Y

    1998-12-15

    Treatment of myoglobin with H2O2 results in covalent alteration of the heme prosthetic group, in part, to protein-bound adducts. These protein-bound heme adducts are known to be redox active and are suspected to participate in oxidative tissue injury. In the course of our studies on the toxicological role of these heme adducts, we sought to develop a sensitive assay for their detection and quantitation. We have discovered that protein-bound heme adducts, due to their inherent peroxidase activity, can be detected with the use of enhanced chemiluminescence detection reagents, following SDS-PAGE and electroblotting. The assay is specific for protein-bound heme adducts as we have identified conditions where noncovalently bound hemes are completely dissociated from the protein during electrophoresis. Signal intensity was quantified by laser densitometry and found to be linear over a concentration range of 0.44-22 pmol of protein-bound heme adduct, which represented a 20-fold greater sensitivity than the currently available HPLC method. Moreover, we have identified tris(2-carboxyethyl)phosphine as a thiol reducing agent that does not interfere with the detection of the heme-mediated peroxidase activity. The current method may be utilized to identify heme-binding regions of proteins in addition to the detection of oxidatively modified myoglobin. PMID:9882417

  14. Sharp Fickian Behavior of Electrogenerated Chemiluminescence Waves in Conjugated Polymer Films

    E-print Network

    Edwards, David A.

    Sharp Fickian Behavior of Electrogenerated Chemiluminescence Waves in Conjugated Polymer Films is the generation of electrogenerated chemiluminescence (ECL) waves. Such waves are sharp and easy to image these devices is through electrogenerated chemiluminescence (ECL),7 which allows optical study of the reduction

  15. Photochemistry and Photobiology. 1996. 63(5): Optically Pumped Chemiluminescence of Indole-3-Acetic Acid

    E-print Network

    Krylov, Sergey

    Photochemistry and Photobiology. 1996. 63(5): Optically Pumped Chemiluminescence of Indole-3-Acetic January 1996 ABSTRACT the main ~ource of al. (4) have ~hown Optically pumped chemiluminescence of indole-3-sen- sitized photooxidation of luminal is a prospective technique for the creation of a new chemiluminescent

  16. Specific Inhibition of Chemiluminescent Activity by Pathogenic Vibrios in Hemocytes of Two Marine Bivalves

    E-print Network

    Paris-Sud XI, Université de

    1 Specific Inhibition of Chemiluminescent Activity by Pathogenic Vibrios in Hemocytes of Two Marine particles were added to the hemocytes and the chemiluminescent (CL) activity of the cells was measured over; Crassostrea gigas; bacteria; chemiluminescence; hemocytes; respiratory burst; Vibrio sp. INTRODUCTION

  17. Experimental and theoretical study of the gas phase reaction of ethynyl radical with methane HCBC CH4

    E-print Network

    Nguyen, Minh Tho

    measured for the ®rst time at higher temperatures by a pulsed laser photolysis/chemiluminescence (PLP-time by the CHA2 D 3 X2 P) chemiluminescence produced by their reaction with O2. The rate coecients kHCC CH4

  18. Enhanced chemiluminescence CdSe quantum dots by histidine and tryptophan

    NASA Astrophysics Data System (ADS)

    Hosseini, Morteza; Ganjali, Mohammad Reza; Jarrahi, Afsaneh; Vaezi, Zahra; Mizani, Farhang; Faridbod, Farnoush

    2014-11-01

    The enhancing effect of histidine and tryptophan on chemiluminescence (CL) of CdSe quantum dots (QDs)-H2O2 system was studied. This reaction is based on the catalytic effect of amino acids, causing a significant increase in the light emission, as a result of the reaction of quantum dots (QDs) with hydrogen peroxide. In the optimum conditions, this method was satisfactorily described by linear calibration curve in the range of 0.66-35.5 ?M and 0.83-35.1 ?M for histidine and tryptophan, respectively. The effect of various parameters such as concentration of CdSe QDs, concentration of H2O2 and concentration of imidazole on the intensity of CL system were studied. The main experimental advantage of the proposed method is it's selective to two amino acids compared with other amino acids.

  19. Photoluminescence, chemiluminescence and anodic electrochemiluminescence of hydrazide-modified graphene quantum dots

    NASA Astrophysics Data System (ADS)

    Dong, Yongqiang; Dai, Ruiping; Dong, Tongqing; Chi, Yuwu; Chen, Guonan

    2014-09-01

    Single-layer graphene quantum dots (SGQDs) were refluxed with hydrazine (N2H4) to prepare hydrazide-modified SGQDs (HM-SGQDs). Compared with SGQDs, partial oxygen-containing groups have been removed from HM-SGQDs. At the same time, a lot of hydrazide groups have been introduced into HM-SGQDs. The introduced hydrazide groups provide HM-SGQDs with a new kind of surface state, and give HM-SGQDs unique photoluminescence (PL) properties such as blue-shifted PL emission and a relatively high PL quantum yield. More importantly, the hydrazide-modification made HM-SGQDs have abundant luminol-like units. Accordingly, HM-SGQDs exhibit unique and excellent chemiluminescence (CL) and anodic electrochemiluminescence (ECL). The hydrazide groups of HM-SGQDs can be chemically oxidized by the dissolved oxygen (O2) in alkaline solutions, producing a strong CL signal. The CL intensity is mainly dependent on the pH value and the concentration of O2, implying the potential applications of HM-SGQDs in pH and O2 sensors. The hydrazide groups of HM-SGQDs can also be electrochemically oxidized in alkaline solutions, producing a strong anodic ECL signal. The ECL intensity can be enhanced sensitively by hydrogen peroxide (H2O2). The enhanced ECL intensity is proportional to the concentration of H2O2 in a wide range of 3 ?M to 500 ?M. The detection limit of H2O2 was calculated to be about 0.7 ?M. The results suggest the great potential applications of HM-SGQDs in the sensors of H2O2 and bio-molecules that are able to produce H2O2 in the presence of enzymes.Single-layer graphene quantum dots (SGQDs) were refluxed with hydrazine (N2H4) to prepare hydrazide-modified SGQDs (HM-SGQDs). Compared with SGQDs, partial oxygen-containing groups have been removed from HM-SGQDs. At the same time, a lot of hydrazide groups have been introduced into HM-SGQDs. The introduced hydrazide groups provide HM-SGQDs with a new kind of surface state, and give HM-SGQDs unique photoluminescence (PL) properties such as blue-shifted PL emission and a relatively high PL quantum yield. More importantly, the hydrazide-modification made HM-SGQDs have abundant luminol-like units. Accordingly, HM-SGQDs exhibit unique and excellent chemiluminescence (CL) and anodic electrochemiluminescence (ECL). The hydrazide groups of HM-SGQDs can be chemically oxidized by the dissolved oxygen (O2) in alkaline solutions, producing a strong CL signal. The CL intensity is mainly dependent on the pH value and the concentration of O2, implying the potential applications of HM-SGQDs in pH and O2 sensors. The hydrazide groups of HM-SGQDs can also be electrochemically oxidized in alkaline solutions, producing a strong anodic ECL signal. The ECL intensity can be enhanced sensitively by hydrogen peroxide (H2O2). The enhanced ECL intensity is proportional to the concentration of H2O2 in a wide range of 3 ?M to 500 ?M. The detection limit of H2O2 was calculated to be about 0.7 ?M. The results suggest the great potential applications of HM-SGQDs in the sensors of H2O2 and bio-molecules that are able to produce H2O2 in the presence of enzymes. Electronic supplementary information (ESI) available: AFM images of SGQDs and HM-SGQDs (Fig. S1), FT-IR spectra of SGQDs and HM-SGQDs (Fig. S2), UV-vis and PL emission spectra of R-SGQDs (Fig. S3), cathodic ECL responses of SGQD, R-SGQDs and HM-SGQDs (Fig. S4), and the pH effect on the anodic ECL responses of HM-SGQDs (Fig. S5). See DOI: 10.1039/c4nr02539c

  20. Determination of serum estradiol levels by radiometric and chemiluminescent techniques.

    PubMed

    Bryant, Carole; Moore, John; Curry, Thomas E

    2009-01-01

    The ability to precisely measure circulating levels of hormones is a foundation of modern endocrinology. For assisted reproductive technologies such as in vitro fertilization (IVF), accurate determination of circulating levels of estradiol are crucial for patient management, retrieval of fertilizable oocytes, and successful pregnancy outcome. For many years, circulating levels of estradiol were determined by radioimmunoassay. More recently, nonradioactive techniques such as ELISAs or chemiluminescent approaches have replaced traditional radioimmunoassays. In the current chapter, we outline the procedures for analysis of circulating levels of estradiol by both radioimmunoassay and chemiluminescent techniques. PMID:19763495

  1. Analysis of chemiluminescence spectra in oxidative degradation of oleic acid

    NASA Astrophysics Data System (ADS)

    Sago, Tomohiro; Ishii, Hiroshi; Hagihara, Hideaki; Takada, Noriyuki; Suda, Hiroyuki

    2013-04-01

    We studied chemiluminescence spectroscopy to develop an evaluation method for organic material degradation. The thermal oxidation of oleic acid as a monounsaturated fatty acid was investigated using chemiluminescence measurements. The obtained spectra indicated that the luminescence consisted of several peaks, including peaks for singlet oxygen and excited carbonyls. A detailed analysis of the nuclear magnetic resonance and infrared absorption spectra allowed us to determine the probable structures of the oxidative products. The relationship between the spectrum and the analyzed structure implied that the wavelength of the carbonyl group emission peak shifted as a result of the structure.

  2. Directional surface plasmon coupled chemiluminescence from nickel thin films: Fixed angle observation

    NASA Astrophysics Data System (ADS)

    Weisenberg, Micah; Aslan, Kadir; Hortle, Elinor; Geddes, Chris D.

    2009-04-01

    Directional surface plasmon coupled chemiluminescence (SPCC) from nickel thin films is demonstrated. Free-space and angular-dependent SPCC emission from blue, green and turquoise chemiluminescent solutions placed onto nickel thin films attached to a hemispherical prism were measured. SPCC emission was found to be highly directional and preferentially p-polarized, in contrast to the unpolarized and isotropic chemiluminescence emission. The largest SPCC emission for all chemiluminescence solutions was observed at a fixed observation angle of 60°, which was also predicted by theoretical Fresnel calculations. It was found that nickel thin films did not have a catalytic effect on chemiluminescence emission.

  3. Carbon nanostructures as catalytic support for chemiluminescence of sulfur compounds in a molecular emission cavity analysis system.

    PubMed

    Safavi, Afsaneh; Maleki, Norouz; Doroodmand, Mohammad Mahdi; Koleini, Mohammad Mehdi

    2009-06-30

    The effect of different substrates including stainless steel, activated carbon, single-walled carbon nanotubes (SWCNTs), multi-walled carbon nanotubes (MWCNTs), fullerenes (C60, C70, etc.) and SWCNTs doped with iron and palladium nanoparticles were compared for catalytic chemiluminescence reaction of sulfur compounds in a flame-containing cavity of molecular emission cavity analysis (MECA) system. Different forms of CNT substrates were fabricated using electric arc-discharge method. The blue emission of excited S2 was monitored using a CCD camera. The results demonstrate that, due to the high surface area, plenty of basal planes, high thermal conductivity, and high flexibility of the carbon nanostructure as appropriate support, carbon nanostructures play an important role in catalytic chemiluminescence emission of sulfur compounds in MECA. Moreover, the presence of metallic nanoparticles doped on carbon nanostructures enhances their catalytic effect. The results revealed that under similar conditions, SWCNTs/Pd doped nanoparticles, SWCNTs/Fe doped nanoparticles, SWCNTs, MWCNTs and fullerenes have the most catalytic effects on chemiluminescence of sulfur compounds, respectively. PMID:19463563

  4. Analyzer for measurement of nitrogen oxide concentration by ozone content reduction in gas using solid state chemiluminescent sensor

    NASA Astrophysics Data System (ADS)

    Chelibanov, V. P.; Ishanin, G. G.; Isaev, L. N.

    2014-05-01

    Role of nitrogen oxide in ambient air is described and analyzed. New method of nitrogen oxide concentration measurement in gas phase is suggested based on ozone concentration measurement with titration by nitrogen oxide. Research of chemiluminescent sensor composition is carried out on experimental stand. The sensor produced on the base of solid state non-activated chemiluminescent composition is applied as ozone sensor. Composition is put on the surface of polymer matrix with developed surface. Sensor compositions includes gallic acid with addition of rodamine-6G. Model of interaction process between sensor composition and ozone has been developed, main products appeared during reaction are identified. The product determining the speed of luminescense appearance is found. This product belongs to quinone class. Then new structure of chemiluminescent composition was suggested, with absence of activation period and with high stability of operation. Experimental model of gas analyzer was constructed and operation algorithm was developed. It was demonstrated that developed NO measuring instrument would be applied for monitoring purposes of ambient air. This work was partially financially supported by Government of Russian Federation, Grant 074-U01

  5. Multiwell cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection: development, characterization and comparison with cooled-CCD luminograph.

    PubMed

    Mirasoli, Mara; Nascetti, Augusto; Caputo, Domenico; Zangheri, Martina; Scipinotti, Riccardo; Cevenini, Luca; de Cesare, Giampiero; Roda, Aldo

    2014-09-01

    We propose a disposable multiwell microcartridge with integrated amorphous silicon photosensors array for bio- and chemiluminescence-based bioassays, where the enzymatic reactions and the detection unit are coupled on the same glass substrate. Each well, made in a polydimethylsiloxane (PDMS) unit, hosts an enzymatic reaction that is monitored by one photosensor of the array. Photosensors were characterized in terms of their dark current background noise and response to different wavelengths of visible light in order to determine their suitability as detection devices for chemical luminescent phenomena. Calibration curves of the photosensors' response to different luminescent systems were then evaluated by using the chemiluminescent reactions catalyzed by alkaline phosphatase and horseradish peroxidase and the bioluminescent reaction catalyzed by firefly luciferase. Limits of detection in the order of attomoles for chemiluminescence enzymes and femtomoles for luciferase and sensitivities in the range between 0.007 and 0.1 pA pmol(-1) L were reached. We found that, without the need of cooling systems, the analytical performances of the proposed cartridge are comparable with those achievable with state-of-the-art thermoelectrically cooled charge-coupled device-based laboratory instrumentation. In addition, thanks to the small amount of generated output data, the proposed device allows the monitoring of long-lasting reactions with significant advantages in terms of data-storage needs, transmission bandwidth, ease of real-time signal processing and limited power consumption. Based on these results, the operation in model bioanalytical assays exploiting luminescent reactions was tested demonstrating that a-Si:H photosensors arrays, when integrated with PDMS microfluidic units, provide compact, sensitive and potentially low-cost microdevices for chemiluminescence and bioluminescence-based bioassays with a wide range of possible applications for in-field and point-of-care bio-analyses. PMID:24965161

  6. Determination of oxides of nitrogen (NO/sub x/) in cigarette smoke by chemiluminescent analysis

    SciTech Connect

    Jenkins, R.A.; Gill, B.E.

    1980-05-01

    The successful application of a commercial chemiluminescent No/sub x/ analyzer to the determination of oxides of nitrogen in cigarette smoke is reported. Individual puffs of the smoke vapor phase are rapidly diluted in an air stream before introduction into the analyzer. This acts to both reduce quenching of the chemiluminescent response by CO/sub 2/ and to prevent side reactions of the NO/sub x/ with vapor phase organic constituents. Sweeping the dilute smoke through a reduced silver-ion exchange resin bed removed a substantial positive interference from hydrogen cyanide. A range of deliveries of 3 to 47 ..mu..mol of NO/sub x/ per cigarette was observed for nine types of experimental cigarettes. Statistically significant differences between NO/sub x/ and NO levels (NO/sub x/ - NO = NO/sub 2/) in smoke were observed in only one type of cigarette, presumably due to large cigarette-to-cigarette variability in constituent deliveries. 2 figures, 3 tables.

  7. A comparison of chemiluminescent acridinium dimethylphenyl ester labels with different conjugation sites.

    PubMed

    Natrajan, Anand; Wen, David

    2015-02-18

    Chemiluminescent acridinium dimethylphenyl esters are highly sensitive labels that are used in automated assays for clinical diagnosis. Light emission from these labels and their conjugates is triggered by treatment with alkaline peroxide. Conjugation of acridinium ester labels is normally done at the phenol. During the chemiluminescent reaction of these acridinium esters, the phenolic ester is cleaved and the light emitting acridone moiety is liberated from its conjugate partner. In the current study, we report the synthesis of three new acridinium esters with conjugation sites at the acridinium nitrogen and compare their properties with that of a conventional acridinium ester with a conjugation site at the phenol. Our study is the first that provides a direct comparison of the emissive properties of acridinium dimethylphenyl esters (free labels and protein conjugates) with different conjugation sites, one where the light emitting acridone remains attached to its conjugate partner versus conventional labeling which results in cleavage of the acridone from the conjugate. Our results indicate that the conjugation at the acridinium nitrogen, which also alters how the acridinium ring and phenol are oriented with respect to the protein surface, has a minimal impact on emission kinetics and emission spectra. However, this mode of conjugation to three different proteins led to a significant increase in light yield which should be useful for improving the assay sensitivity. PMID:25581208

  8. Bio\\/chemical microsystem designed for wafer scale testing

    Microsoft Academic Search

    Anders M. Jorgensen; Klaus B. Mogensen; Weimin Rong; Pieter Telleman; Joerg P. Kutter

    2001-01-01

    We have designed a bio\\/chemical microsystem for online monitoring of glucose concentrations during fermentation. The system contains several passive microfluidic components including an enzyme reactor, a flow lamination part and a detector. Detection is based on the reaction of hydrogen peroxide, that is produced from glucose in an enzyme reactor, with luminol. This chemiluminescent reaction generates light that is detected

  9. Reaction of barium atoms with N/sub 2/O clusters

    SciTech Connect

    Visticot, J.P.; Mestdagh, J.M.; Alcaraz, C.; Cuvellier, J.; Berlande, J.

    1988-03-01

    The chemiluminescence of the reaction of barium atoms and N/sub 2/O molecules is observed in a crossed molecular beam experiment. The conditions of the molecular N/sub 2/O beam are varied so that this beam contains no, or many large, N/sub 2/O clusters. No additional chemiluminescence occurs when the cluster concentration is increased. The large difference in electronic structure between monomers and polymers, which has been shown by Echt et al. by measurement of electron attachment, suggests that a different reaction channel yielding BaO solvated in a N/sub 2/O cluster is responsible for this absence of chemiluminescence.

  10. TECHNICAL ASSISTANCE DOCUMENT FOR THE CHEMILUMINESCENCE MEASUREMENT OF NITROGEN DIOXIDE

    EPA Science Inventory

    Gas phase chemiluminescence has been designated as the reference measurement principle for the measurement of nitrogen dioxide (NO2) in the ambient atmosphere. Continuous analyzers based on this measurement principle may be calibrated with NO2 either from the gas phase titration ...

  11. Chemiluminescent Emission Data For Kinetic Modeling Of Ethanol Combustion

    Microsoft Academic Search

    Leandro H. Benvenutti; Carla S. T. Marques; Celso A. Bertran

    2004-01-01

    Kinetic modeling is a powerful tool for combustion investigations and has been widely used. The validation of the model is a very important element of the work, to achieve realistic results, and it normally uses concentration profiles of some participant species. In this work, a kinetic model was validated using the chemiluminescent emissions of excited radicals produced in ethanol vapor

  12. Studies on water treeing and chemiluminescence on irradiated polyethylene

    SciTech Connect

    Notingher, P.V.; Ciuprina, F.; Radu, I. [Politehnica Univ. of Bucharest (Romania); Jipa, S.; Setnescu, T.; Setnescu, R. [ICPE, Bucharest (Romania); Filippini, J.C.; Gosse, B. [CNRS, Grenoble (France). Lab. d`Electrostatique et de Materiaux; Mihalcea, T. [Univ. of Bucharest (Romania)

    1996-12-31

    The effect of {gamma}-radiations on the growth kinetics of water trees is examined using the CNRS laboratory model in LDPE samples of different origins. The effect of radiations on the material is studied from crosslinking degree measurements, IRTF spectroscopy and chemiluminescence. Correlations between the non-uniformity of the degradation and the growth of water trees are observed.

  13. Chemiluminescence. (Latest citations from the Compendex database). Published Search

    SciTech Connect

    Not Available

    1993-05-01

    The bibliography contains citations concerning several forms of chemiluminescence, including stress-induced, laser-induced, and electrogenerated. Air pollution measurement instrumentation is described, and measurements of hydrogen peroxide, nitric oxide, vinyl chloride, and other atmospheric contaminants are discussed. (Contains 250 citations and includes a subject term index and title list.)

  14. Determination of pentachlorophenol and 1-naphthol by peroxyoxalate chemiluminescence

    SciTech Connect

    Chimeno, J.; von Wandruszka, R. (Univ. of Idaho, Moscow (United States))

    1989-01-01

    Peroxyoxalate chemiluminescence is used for the determination of pentachlorophenol and 1-naphthol in soil. 1-Naphthol is brominated to make it amenable to the procedure. Calibration curves are linear over concentration ranges spanning four orders of magnitude and limits of detection are at the micromolar level. The processes can be carried out in the soil without prior extraction of the analytes.

  15. Enhancement of the chemiluminescence of penicillamine and ephedrine after derivatization with aldehydes using tris(bipyridyl)ruthenium(II) peroxydisulfate system and its analytical application

    Microsoft Academic Search

    Fakhr Eldin O. Suliman; Mohammed M. Al-Hinai; Salma M. Z. Al-Kindy; Salama B. Salama

    2008-01-01

    A novel sequential injection (SI) method was developed for the determination of penicillamine (PA) and ephedrine (EP) based on the reaction of these drugs with tris(bipyridyl)ruthenium(II) (Ru(bpy)32+) and peroxydisulfate (S2O82?) in the presence of light. Derivatization of PA and EP with aldehydes has resulted in a significant enhancement of the chemiluminescence emission signal by at least 25 times for PA

  16. Chemiluminescence determination of some fluoroquinolone derivatives in pharmaceutical formulations and biological fluids using [Ru(bipy) 3 2+]–Ce(IV) system

    Microsoft Academic Search

    Fatma A. Aly; Salma A. Al-Tamimi; Abdulrahman A. Alwarthan

    2001-01-01

    A new chemiluminescence (CL) method using flow injection has been described for the rapid and sensitive determination of three fluoroquinolone derivatives, namely ofloxacin, norfloxacin and ciprofloxacin hydrochloride. The method is based on the CL reaction of the studied fluoroquinolones with tris(2,2?-bipyridyl)ruthenium(II) [Ru(bipy)32+] and Ce(IV) in sulfuric acid medium. Under the optimum conditions, the CL intensity is proportional to the concentration

  17. Simultaneous detection of single attoliter droplet collisions by electrochemical and electrogenerated chemiluminescent responses.

    PubMed

    Dick, Jeffrey E; Renault, Christophe; Kim, Byung-Kwon; Bard, Allen J

    2014-10-27

    We provide evidence of single attoliter oil droplet collisions at the surface of an ultra-microelectrode (UME) by the observation of simultaneous electrochemical current transients (i-t curves) and electrogenerated chemiluminescent (ECL) transients in an oil/water emulsion. An emulsion system based on droplets of toluene and tri-n-propylamine (2:1 v/v) emulsified with an ionic liquid and suspended in an aqueous continuous phase was formed by ultrasonification. When an ECL luminophore, such as rubrene, is added to the emulsion droplet, stochastic events can be tracked by observing both the current blips from oxidation at the electrode surface and the ECL blips from the follow-up ECL reaction, which produces light. This report provides a means of studying fundamental aspects of electrochemistry using the attoliter oil droplet and offers complementary analytical techniques for analyzing discrete collision events, size distribution of emulsion systems, and individual droplet electroactivity. PMID:25213468

  18. Peroxyoxalate chemiluminescence enhanced by oligophenylenevinylene fluorophores in the presence of various surfactants.

    PubMed

    Motoyoshiya, Jiro; Takigawa, Setsuko

    2014-11-01

    The effect of several surfactants on peroxyoxalate chemiluminescence (PO-CL) using oligophenylenevinylene fluorophores was investigated. Among several oligophenylenevinylenes consisting of stilbene units, linearly conjugated ones, such as distyrylbenzene and distyrylstilbene, effectively enhanced PO-CL efficiency. Various effects of anionic, cationic, amphoteric and non-ionic surfactants on the CL efficiency of PO-CL were determined using three oxalates and the distyrylbenzene fluorophore. Anionic and non-ionic surfactants effectively enhanced CL efficiency, in contrast to the negative effect of cationic and amphoteric surfactants. Non-ionic surfactants were also effective in CL reactions of oxalates bearing dodecyl ester groups by the hydrophobic interaction between their alkyl chains. Considering these results, the surfactants not only increase the concentrations of water-insoluble interacting species in the hydrophobic micelle cores, but also control rapid degradation of the oxalates by alkaline hydrolysis. PMID:24425305

  19. Numerical evaluation of equivalence ratio measurement using OH{sup *} and CH{sup *} chemiluminescence in premixed and non-premixed methane-air flames

    SciTech Connect

    Panoutsos, C.S.; Hardalupas, Y.; Taylor, A.M.K.P. [Department of Mechanical Engineering, Imperial College London, Exhibition Road, London SW7 2BX (United Kingdom)

    2009-02-15

    This work presents results from detailed chemical kinetics calculations of electronically excited OH (A{sup 2}{sigma}, denoted as OH{sup *}) and CH (A{sup 2}{delta}, denoted as CH{sup *}) chemiluminescent species in laminar premixed and non-premixed counterflow methane-air flames, at atmospheric pressure. Eight different detailed chemistry mechanisms, with added elementary reactions that account for the formation and destruction of the chemiluminescent species OH{sup *} and CH{sup *}, are studied. The effects of flow strain rate and equivalence ratio on the chemiluminescent intensities of OH{sup *}, CH{sup *} and their ratio are studied and the results are compared to chemiluminescent intensity ratio measurements from premixed laminar counterflow natural gas-air flames. This is done in order to numerically evaluate the measurement of equivalence ratio using OH{sup *} and CH{sup *} chemiluminescence, an experimental practise that is used in the literature. The calculations reproduced the experimental observation that there is no effect of strain rate on the chemiluminescent intensity ratio of OH{sup *} to CH{sup *}, and that the ratio is a monotonic function of equivalence ratio. In contrast, the strain rate was found to have an effect on both the OH{sup *} and CH{sup *} intensities, in agreement with experiment. The calculated OH{sup *}/CH{sup *} values showed that only five out of the eight mechanisms studied were within the same order of magnitude with the experimental data. A new mechanism, proposed in this work, gave results that agreed with experiment within 30%. It was found that the location of maximum emitted intensity from the excited species OH{sup *} and CH{sup *} was displaced by less than 65 and 115 {mu}m, respectively, away from the maximum of the heat release rate, in agreement with experiments, which is small relative to the spatial resolution of experimental methods applied to combustion applications, and, therefore, it is expected that intensity from the OH{sup *} and CH{sup *} excited radicals can be used to identify the location of the reaction zone. Calculations of the OH{sup *}/CH{sup *} intensity ratio for strained non-premixed counterflow methane-air flames showed that the intensity ratio takes different values from those for premixed flames, and therefore has the potential to be used as a criterion to distinguish between premixed and non-premixed reaction in turbulent flames. (author)

  20. Detection of lanthanides and actinides in solutions based on laser-induced luminescence and chemiluminescence

    NASA Astrophysics Data System (ADS)

    Izosimov, I. N.; Firsin, N. G.; Gorshkov, N. G.; Nekhoroshkov, S. N.

    2014-06-01

    This work is devoted to applications of the time-resolved laser- induced luminescence spectroscopy and time-resolved laser-induced chemiluminescence spectroscopy for detection of lanthanides and actinides. The limit of detection (LOD) of some lanthanides and actinides in solutions by the time-resolved laser luminescence spectroscopy is up to 10-13 mole/liter. Unfortunately, Pu, Np, and some U compounds do not produce luminescence in solutions, but when excited by laser radiation, they can induce chemiluminescence. The characteristics of chemiluminescence induced by excited lanthanide and actinide complexes in solutions are considered. A key problem of chemiluminescence application for lanthanide and actinide detection in solutions is the increase of the detection selectivity. This problem can be solved by using the multistep schemes of chemiluminescence excitation. It is shown that the time-resolved luminescence laser spectroscopy and chemiluminescence laser spectroscopy can be successfully used for detection of lanthanides and actinides in solutions.

  1. Electrogenerated chemiluminescence of isoluminol and acridinium esters in flow injection analysis and HPLC. [HPLC (high-performance liquid chromatography)

    SciTech Connect

    Littig, J.H.

    1992-01-01

    Electrogenerated chemiluminescence (ECL) is the process in which electrochemically generated reactants undergo high energy electron transfer reactions in solution to generate excited state molecules which relax to the ground state with emission of light. This research focused on the study and application of the electrogenerated chemiluminescence reactions of isoluminol and acridinium esters. Initial research involved the ECL reaction of an isoluminol derivative, 4-isothiocyanatophythalhydrazide (ILITC). The derivative was synthesized and characterized prior to use as a label for amino acid determination. Chromatographic separation of ILITC-amino acids preceded the ECL detection. A drastic reduction in ECL efficiency was observed upon coupling of ILITC to the amino acid analytes. Experiments suggested that although an ILITC-amino acid coupling reaction occurs, the resultant product has lost CL activity. Additional studies illustrated a correlation between CL activity and the conditions used to couple ILITC to the analyte. In spite of difficulties associated with loss of CL activity, the ECL determination of ILITC-amino acids (1 nmol) and the feasibility of on-line analyte derivatization were demonstrated. The ECL reaction of acridinium esters was studied and shown to proceed via the electrochemical generation of hydrogen peroxide. In further fundamental studies of the acridinium ester decomposition reactions, kinetic parameters associated with this decay were determined and applied in a model for pH optimization. The use of ECL for the determination of acridinium ester-labeled species was demonstrated via the successful quantitation (to 50 fmol) of lysine labeled with a hydroxysuccinimide acridinium ester. Instrumental configurations for use of ECL detection in immunoassay were validated; however, loss of ECL signal in the presence of BSA and human TSH antibodies hindered application of acridinium ester ECL detection to model immunoassay reactions.

  2. Crystallochemiluminescence of solutions

    NASA Astrophysics Data System (ADS)

    Gus'kov, A. P.; Nekrasova, L. P.; Gornakova, A. S.; Shikunova, I. A.

    2014-09-01

    It is shown that the chemiluminescence intensity from luminol solutions reaches a maximum when the latter are crystallized. This phenomenon is explained by the complex dynamics of the phase transition, chemical reactions, and degradation of electronic excitation energy. Luminescence of new type, called crystallochemiluminescence, is revealed.

  3. Chemiluminescence flow sensor with immobilized reagent for the determination of pyrogallol based on potassium hexacyanoferrate(III) oxidation.

    PubMed

    Chen, Jianchun; Bai, Jun

    2008-12-01

    A novel chemiluminescence (CL) flow-through sensor for the determination of pyrogallol has been developed. The method is based on the reaction between pyrogallol and potassium hexacyanoferrate(III) in sodium hydroxide solution. Potassium hexacyanoferrate(III) involved in the CL reaction was electrostatically immobilized on anion-exchange resin packed in a column. Pyrogallol was sensed by the CL reaction between pyrogallol and potassium hexacyanoferrate(III) which was eluted from the ion-exchange column through sodium phosphate injection. The CL emission allows quantitation of pyrogallol concentration in the range 0.01-3.8 microg/mL with a detection limit (3 sigma) of 0.003 microg/mL and a sample throughput of 118 h(-1). The relative standard deviation (n=7) was 2.2% for 0.2 microg/mL of pyrogallol. The influence of foreign compounds was tested. PMID:18373950

  4. A novel chemiluminescent ELISA for detecting furaltadone metabolite, 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) in fish, egg, honey and shrimp samples.

    PubMed

    Liu, Ying-Chun; Jiang, Wei; Chen, Yong-Jun; Xiao, Yan; Shi, Jin-Lei; Qiao, Yuan-Biao; Zhang, Hua-Jing; Li, Tao; Wang, Quan

    2013-09-30

    In this study, an indirect competitive enzyme-linked immunosorbent assay with chemiluminescent (CLELISA) detection for 3-amino-5-morpholinomethyl-2-oxazolidone (AMOZ) was developed. A monoclonal antibody (MAb) against AMOZ was prepared through immunizing BALB/c mice with 4-carboxybenzaldehyle derivatized AMOZ (CPAMOZ), conjugated with bovine serum albumin (BSA) as antigen. The effects of the substrates luminol, p-iodophenol and urea peroxide on the performance of the assay were studied and optimized. In addition, the specificity of the MAb, estimated as the cross-reactivity values with 4-nitrobenzaldehyde derivatized AMOZ (NPAMOZ), CPAMOZ and AMOZ, was 100%, 27.45% and 0.18%, respectively. The sensitivity of the developed CLELISA was estimated as 50% inhibitory concentration (IC50) value (0.14?g/l) with a linear working range between 0.03 and 64?g/l, and a limit of detection of 0.01?g/l. The CLELISA described in this study was 5-fold more sensitive than the indirect competitive ELISA previously developed in our laboratory. Finally, this new CLELISA was compared with a commercial kit to detect NPAMOZ in spiked fish, shrimp, honey and egg samples. The recovery values from four spiked fish, shrimp, honey and egg samples with different concentrations of NPAMOZ in CLELISA were 92.1-107.7%. Thus, the immunoassay method described here has a broad detection range and high sensitivity and is a valid and cost-effective means for high throughput monitoring of residual AMOZ levels in fish, shrimps, honey and eggs with potential applications in other animal tissues. PMID:23810835

  5. Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen.

    PubMed

    Liu, Ruping; Wang, Cheng; Jiang, Quan; Zhang, Wei; Yue, Zhao; Liu, Guohua

    2013-11-01

    We report a magnetic-particle (MMP)-based chemiluminescence enzyme immunoassay (CLEIA) for free prostate-specific antigen (f-PSA) in human serum. In this method, the f-PSA is sandwiched between the anti-PSA antibody coated MMPs and alkaline phosphatase (ALP)-labeled anti-f-PSA antibody. The signal produced by the emitted photons from the chemiluminescent substrate (4-methoxy-4-(3-phosphatephenyl)-spiro-(1,2-dioxetane-3,2'-adamantane)) is directly proportional to the amount of f-PSA in a sample. The present MMP-based assay can detect f-PSA in the range of 0.1-30 ng mL(-1) with the detection limit of 0.1 ng mL(-1). The linear detection range could match the concentration range within the "diagnostic gray zone" of serum f-PSA levels (4-10 ng mL(-1)). The detection limit was sufficient for measuring clinically relevant f-PSA levels (>4 ng mL(-1)). Furthermore, the method was highly selective; it was unaffected by cross-reaction with human glandular kallikrein-2, a kallikrein-like serine protease that is 80% similar to f-PSA. The proposed method was finally applied to determine f-PSA in 40 samples of human sera. Results obtained using the method showed high correlation with those obtained using a commercially available microplate CLEIA kit (correlation coefficient, 0.9821). This strategy shows great potential application in the fabrication of diagnostic kits for determining f-PSA in serum. PMID:24139579

  6. Spectral analysis of cavity chemiluminescence of a combustion-driven HF laser fueled by NF3

    NASA Astrophysics Data System (ADS)

    Li, Liucheng; Duo, Liping; Wang, Yuanhu; Tang, Shukai; Yu, Haijun; Li, Guofu; Wang, Jian

    2015-02-01

    The visible and near infrared spectra of cavity chemiluminescence of a combustion driven HF laser fueled by NF3 were collected and analyzed. The spectral line at 529 nm for the green chemiluminescence was attributed to electronic excited NF molecules in b1? state, i.e. NF(b). The diffuse bands from 570 nm to 700 nm were attributed to the N2(B-A) emission. The spectral lines from 850 nm to 1000 nm were attributed to the HF ?? = 3 emission bands. At the end of every experiment, the spectral line at 874 nm would be observed, which was attributed to the electronic excited NF molecules in a1 ? state, i.e. NF(a). The NF(a-X) emission was found experimentally to be always avoiding the HF?? = 3 emission bands. It was also found experimentally that the NF(b-X) emission always accompanied the HF ?? = 3 emission bands and their emission intensities had the same trends as a function of experimental time. Whereas the NF(a) molecules was produced in the optical cavity directly by the reaction of H atoms with NF2 molecules in the incomplete combustion effluents, the NF(b) molecules were suggested to be produced mainly by the near resonant energy transfer from vibrational excited HF(v<=2) molecules to NF(a) molecules. In other words, the vibrational excited state HF(v<=2) molecules can be efficiently deactivated by the NF(a) molecules by near resonant V-E energy transfer process. Therefore we concluded that incomplete dissociation of NF3 might be harmful to the HF(v<=2) population.

  7. Orderly arranged fluorescence dyes as a highly efficient chemiluminescence resonance energy transfer probe for peroxynitrite.

    PubMed

    Wang, Zhihua; Teng, Xu; Lu, Chao

    2015-03-17

    Chemiluminescence (CL) probes for reactive oxygen species (ROS) are commonly based on a redox reaction between a CL reagent and ROS, leading to poor selectivity toward a specific ROS. The energy-matching rules in the chemiluminescence resonance energy transfer (CRET) process between a specific ROS donor and a suitable fluorescence dye acceptor is a promising method for the selective detection of ROS. Nevertheless, higher concentrations of fluorescence dyes can lead to the intractable aggregation-caused quenching effect, decreasing the CRET efficiency. In this report, we fabricated an orderly arranged structure of calcein-sodium dodecyl sulfate (SDS) molecules to improve the CRET efficiency between ONOOH* donor and calcein acceptor. Such orderly arranged calcein-SDS composites can distinguish peroxynitrite (ONOO(-)) from a variety of other ROS owing to the energy matching in the CRET process between ONOOH* donor and calcein acceptor. Under the optimal experimental conditions, ONOO(-) could be assayed in the range of 1.0-20.0 ?M, and the detection limit for ONOO(-) [signal-to-noise ratio (S/N) = 3] was 0.3 ?M. The proposed strategy has been successfully applied in both detecting ONOO(-) in cancer mouse plasma samples and monitoring the generation of ONOO(-) from 3-morpholinosydnonimine (SIN-1). Recoveries from cancer mouse plasma samples were in the range of 96-105%. The success of this work provides a unique opportunity to develop a CL tool to monitor ONOO(-) with high selectivity in a specific manner. Improvement of selectivity and sensitivity of CL probes holds great promise as a strategy for developing a wide range of probes for various ROS by tuning the types of fluorescence dyes. PMID:25693881

  8. Aggregation-induced emission: a simple strategy to improve chemiluminescence resonance energy transfer.

    PubMed

    Zhang, Lijuan; He, Nan; Lu, Chao

    2015-01-20

    The emergence of aggregation-induced emission (AIE) has opened up a new avenue for scientists. There is a great demand for the development of a new generation chemiluminescence resonance energy transfer (CRET) acceptors with AIE characteristics due to the aggregation-caused chemiluminescence (CL) quenching effect commonly observed in the conventional fluorophore CL acceptors at high concentrations. However, the systematical studies involving in AIE-amplified CL are still scarce. Herein, it is the first report that the gold nanocluster aggregates (a type of well-defined AIE molecules) are used to study their influence on the bis(2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 CL reaction. Interestingly, the AIE molecules in the diluted solution are unable to boost the CL signal of the TCPO-H2O2 system, but their aggregates display a strongly enhanced CL emission compared to their counterparts of fluorophore molecules, thanks to the unique AIE effect of gold nanoclusters. In comparison to rhodamine B with the aid of an imidazole catalyst, the detection limit of the gold nanocluster aggregate-amplified CL probe for H2O2 (S/N = 3) is low in the absence of any catalyst. Finally, the other two typical AIE molecules, Au(I)-thiolate complexes and 9,10-bis[4-(3-sulfonatopropoxyl)-styryl]anthracene (BSPSA), are investigated to verify the generality of the AIE molecule-amplified CL emissions. These results demonstrate effective access to highly fluorescent AIE molecules with practical applications in avoiding the aggregation-induced CL quenching at high concentrations, which can be expected to provide a novel and sensitive platform for the CL amplified detection. PMID:25526522

  9. Chemiluminescence-based multivariate sensing of local equivalence ratios in premixed atmospheric methane-air flames

    SciTech Connect

    Tripathi, Markandey M.; Krishnan, Sundar R.; Srinivasan, Kalyan K.; Yueh, Fang-Yu; Singh, Jagdish P.

    2012-03-01

    Chemiluminescence emissions from OH*, CH*, C2, and CO2 formed within the reaction zone of premixed flames depend upon the fuel-air equivalence ratio in the burning mixture. In the present paper, a new partial least square regression (PLS-R) based multivariate sensing methodology is investigated and compared with an OH*/CH* intensity ratio-based calibration model for sensing equivalence ratio in atmospheric methane-air premixed flames. Five replications of spectral data at nine different equivalence ratios ranging from 0.73 to 1.48 were used in the calibration of both models. During model development, the PLS-R model was initially validated with the calibration data set using the leave-one-out cross validation technique. Since the PLS-R model used the entire raw spectral intensities, it did not need the nonlinear background subtraction of CO2 emission that is required for typical OH*/CH* intensity ratio calibrations. An unbiased spectral data set (not used in the PLS-R model development), for 28 different equivalence ratio conditions ranging from 0.71 to 1.67, was used to predict equivalence ratios using the PLS-R and the intensity ratio calibration models. It was found that the equivalence ratios predicted with the PLS-R based multivariate calibration model matched the experimentally measured equivalence ratios within 7%; whereas, the OH*/CH* intensity ratio calibration grossly underpredicted equivalence ratios in comparison to measured equivalence ratios, especially under rich conditions ( > 1.2). The practical implications of the chemiluminescence-based multivariate equivalence ratio sensing methodology are also discussed.

  10. Low-level chemiluminescence from Drosophila melanogaster fed with chemical mutagens polycyclic aromatic hydrocarbon quinones and a carcinogenic bracken fern.

    PubMed

    Sato, T; Inaba, H; Kawai, K; Furukawa, H; Hirono, I; Miyazawa, T

    1991-11-01

    The spontaneous photon emission (chemiluminescence) from Drosophila melanogaster fed chemical mutagens, polycyclic aromatic hydrocarbon quinones, and a carcinogenic bracken fern was studied. The fly chemiluminescence was evidently enhanced by mutagen or carcinogen administration and was increased proportionally to the administered amount of tested compound. Strong chemiluminescence was observed especially at the larval stage. Living larvae emitted stronger chemiluminescence than their homogenate. The chemiluminescence from Drosophila melanogaster fed polycyclic aromatic hydrocarbon quinones showed a linear relation with the mutation frequency in the Drosophila wing spot test. The chemiluminescence from flies fed a bracken fern decreased by the addition of free radical scavengers and active oxygen quenchers. The phosphatidylcholine hydroperoxide concentration in the flies was increased proportionally with the chemiluminescence intensity. It seems that the free radical formation is stimulated as shown by the enhanced chemiluminescence in mutagen- or carcinogen-dosed flies, and as a result, lipid peroxide accumulation accompanies mutation in Drosophila melanogaster. PMID:1944380

  11. Flow injection chemiluminescence determination of hydrazine by oxidation with chlorinated isocyanurates.

    PubMed

    Safavi, Afsaneh; Karimi, Mohammad Ali

    2002-10-16

    A rapid and sensitive flow injection chemiluminescence (CL) method is described for the determination of hydrazine based on the CL generated during its reaction with either sodium dichloroisocyanurate (SDCC) or trichloroisocyanuric acid (TCCA) in alkaline medium. The emission intensity is greatly enhanced if dichlorofluorescein (DCF) as sensitizer is present in the reaction medium. The presence of citrate prevents the precipitation of some cations in the reaction medium and also causes an enhancement in emission intensity. The effect of analytical and flow injection variables on these CL systems and determination of hydrazine are discussed. The optimum parameters for the determination of hydrazine were studied and were found to be the following: SDCC and TCCA both 1x10(-3) M; NaOH, 2x10(-1) M; DCF, 5x10(-6) M; citrate, 1x10(-3) M and flow rate, 3.8 ml min(-1). The optimized method yielded 3sigma detection limits of 2x10(-7) and 3x10(-7) M for hydrazine with SDCC and TCCA oxidants, respectively. The method is simple, fast, sensitive, and precise and was applied to the determination of hydrazine in water samples. PMID:18968808

  12. Aggregation-induced structure transition of protein-stabilized zinc/copper nanoclusters for amplified chemiluminescence.

    PubMed

    Chen, Hui; Lin, Ling; Li, Haifang; Li, Jianzhang; Lin, Jin-Ming

    2015-02-24

    A stable, water-soluble fluorescent Zn/Cu nanocluster (NC) capped with a model protein, bovine serum albumin (BSA), was synthesized and applied to the reaction of hydrogen peroxide and sodium hydrogen carbonate. A significantly amplified chemiluminescence (CL) from the accelerated decomposition of peroxymonocarbonate (HCO4(-)) by the nanosluster was observed. The CL reaction led to a structure change of BSA and aggregation of Zn/Cu NCs. In the presence of H2O2, Zn/Cu-S bonding between BSA scaffolds and the encapsulated Zn/Cu@BSA NC was oxidized to form a disulfide product. Zn/Cu@BSA NCs were prone to aggregate to form larger nanoparticles without the protection of scaffolds. It is revealed that the strong CL emission was initiated from the catalysis of Zn/Cu@BSA NC and the surface plasmon coupling of the formed Zn/Cu nanoparticles in a single chemical reaction. This amplified CL was successfully exploited for selective sensing of hydrogen peroxide in environmental samples. PMID:25647180

  13. Proceedings of the Combustion Institute, Volume 29, 2002/pp. 16631670 CHEMILUMINESCENT OH* AND CH* FLAME STRUCTURE AND

    E-print Network

    Tse, Stephen D.

    1663 Proceedings of the Combustion Institute, Volume 29, 2002/pp. 1663­1670 CHEMILUMINESCENT OH to the chemiluminescent species. Theexperimental results were then compared with computations allowing for detailed the adequacy of the chemistry and the experimental approach. For hydrogen/methane flames, OH* chemiluminescence

  14. Chemiluminescence and superoxide anion production by leukocytes from chronic hemodialysis patients

    Microsoft Academic Search

    Elizabeth E Ritchey; John D Wallin; Sudhir V Shah

    1981-01-01

    Chemiluminescence and superoxide anion production by leukocytes from chronic hemodialysis patients. During phagocytosis or in response to a soluble stimulus, polymorphonuclear leukocytes (PMN) undergo a burst of oxidative metabolism involved intimately in antimicrobial activity. Superoxide anion produced during the burst is bactericidal either directly or as an intermediate metabolite. In addition, stimulated PMN's emit light or chemiluminescence (CL). CL is

  15. Chemiluminescence enzyme immunoassay using ProteinA-bacterial magnetite complex

    NASA Astrophysics Data System (ADS)

    Matsunaga, Tadashi; Sato, Rika; Kamiya, Shinji; Tanaka, Tsuyosi; Takeyama, Haruko

    1999-04-01

    Bacterial magnetic particles (BMPs) which have ProteinA expressed on their surface were constructed using magA which is a key gene in BMP biosynthesis in the magnetic bacterium Magnetospirillum sp. AMB-1. Homogenous chemiluminescence enzyme immunoassay using antibody bound ProteinA-BMP complexes was developed for detection of human IgG. A good correlation between the luminescence yield and the concentration of human IgG was obtained in the range of 1-10 3 ng/ml.

  16. Chemiluminescence detection of opium poppy (Papaver somniferum) alkaloids.

    PubMed

    Francis, Paul S; Adcock, Jacqui L; Costin, Jason W; Purcell, Stuart D; Pfeffer, Frederick M; Barnett, Neil W

    2008-11-01

    A review with 98 references. The determination of the opium poppy (Papaver somniferum) alkaloids and their semi-synthetic derivatives has important applications in industrial process monitoring, clinical analysis and forensic science. Liquid-phase chemiluminescence reagents such as tris(2,2'-bipyridyl)ruthenium(II) and acidic potassium permanganate exhibit remarkable sensitivity and complementary selectivity for many P. somniferum alkaloids, which has been exploited in the development of a range of analytical procedures using flow analysis, high-performance liquid chromatography, capillary electrophoresis and microfluidic instrumentation. PMID:18707837

  17. Chemiluminescent determination of EDTA and related compounds using tris(2,2'-bipyridyl)ruthenium(III) photogenerated on-line.

    PubMed

    Pérez-Ruiz, Tomás; Martínez-Lozano, Carmen; García-Martínez, María Dolores

    2008-06-01

    A rapid and sensitive chemiluminescence method using flow-injection has been developed for the determination of EDTA. The method is based on the chemiluminescent reaction of EDTA with tris(2,2'-bipyridyl)ruthenium(III), which is generated on-line through the photooxidation of tris(2,2'-bipyridyl)ruthenium(II) with peroxydisulfate. After optimizing the different experimental parameters, a calibration graph was obtained over the concentration range of 7 x 10(-8) M to 3 x 10(-6) M, with a minimum detectability of 7.2 x 10(-9) M (S/N = 3) observed. The correlation coefficient was 0.9992 (n = 8). The repeatability was 0.88 % (for a level of 4 x 10(-7) M) expressed as the relative standard deviation (n = 10), and the reproducibility (studied on five consecutive days) was 1.5%. The method was successfully applied to the determination of EDTA in ophthalmic collyrium and sauce samples. The method is also useful for determining other aminopolycarboxylic acids, such as NTA, EGTA, DTPA, DCTA and EDDS. PMID:18259735

  18. A study of interferences in ozone UV and chemiluminescence monitors

    SciTech Connect

    Hudgens, E.E.; Kleindienst, T.E. [ManTech Environmental Technology, Inc., Research Triangle Park, NC (United States); McElroy, F.F. [Environmental Protection Agency, Research Triangle Park, NC (United States). Atmospheric Research and Exposure Assessment Laboratory; Ollison, W.M. [American Petroleum Inst., Washington, DC (United States)

    1994-12-31

    A study was conducted to examine interferences and other measurement anomalies in chemiluminescence and ultraviolet ozone monitors. Previous results had show that there was a positive deviation in the chemiluminescence monitors and no direct interference with ultraviolet monitors due to the presence of water at non-condensing concentrations. The present study continues this effort, examining both potential positive and negative effects of moisture and other interferences on these monitors. Aromatic compounds and their oxidation products could potentially show a positive interference with ultraviolet monitors, and test measurements were made with aromatics such as toluene, benzaldehyde, and nitrotoluene to determine their possible retention in the ozone scrubber and their absorption in the cell as a function of the humidity. A detailed examination of the scrubbers used in ultraviolet ozone monitors has also been undertaken. Ozone scrubbers that have shown anomalous behavior in the field have been studied in various reduced-efficacy modes under controlled laboratory conditions. Longer term tests of unused scrubbers for possible ozone breakthrough under exposure to various simulated field conditions were initiated.

  19. Determination of indole-3-acetic acid and indole-3-butyric acid in mung bean sprouts using high performance liquid chromatography with immobilized Ru(bpy) 3 2+–KMnO 4 chemiluminescence detection

    Microsoft Academic Search

    Zhijun Xi; Zhujun Zhang; Yonghua Sun; Zuolong Shi; Wei Tian

    2009-01-01

    A novel method for determination of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) in an extract from mung bean sprouts using high performance liquid chromatography (HPLC) with chemiluminescence (CL) detection is described. The method is based on the CL reaction of auxin (indole-3-acetic acid and indole-3-butyric acid) with acidic potassium permanganate (KMnO4) and tris(2,2?-bipyridyl)ruthenium(II), which was immobilized on the cationic

  20. Resolution of Ofloxacin–Ciprofloxacin and Ofloxacin–Norfloxacin Binary Mixtures by Flow-Injection Chemiluminescence in Combination with Partial Least Squares Multivariate Calibration

    Microsoft Academic Search

    J. A. Murillo; A. Alañón Molina; A. Muñoz de la Peña; I. Durán Merás; A. Jiménez Girón

    2007-01-01

    A flow-injection chemiluminescence (CL) method is described for the determination of ciprofloxacin (CIP), norfloxacin (NOR)\\u000a and ofloxacin (OFL), commonly used antibiotics of the fluoroquinolones family. The method is based on the CL reaction of the\\u000a fluoroquinolones with tris(2,2?-bipyridyl) ruthenium(II) and Ce (IV), in sulfuric acid medium. The maximum CL emission, given\\u000a at 0.45 min for CIP, at 0.35 min for NOR and

  1. Chemiluminescence by the interaction of XeO{sub 3} and the products of photolysis of uranyl solutions in sulfuric acid

    SciTech Connect

    Khamidullina, L.A.; Lotnik, S.V.; Kazakov, V.P. [Institute of Organic Chemistry, Ufa (Russian Federation)

    1994-10-01

    Using the chemiluminescence oxidation of U(IV) and H{sub 2}O{sub 2} with xenon trioxide as a model, it has been found that during the photolysis of solutions of UO{sub 2}SO{sub 4} in sulfuric acid in the absence of any organic compounds, the accumulation of U(SO{sub 4}){sub 2} and H{sub 2}O{sub 2} takes place as a result of the reaction of the primary products of the photoreduction of uranyl ion, i.e., UO{sub 2}{sup +} and the OH radical.

  2. Simple and sensitive method for determination of glycoalkaloids in potato tubers by high-performance liquid chromatography with chemiluminescence detection.

    PubMed

    Kodamatani, Hitoshi; Saito, Keiitsu; Niina, Nobumitsu; Yamazaki, Shigeo; Tanaka, Youichi

    2005-12-23

    A novel, simple and sensitive high-performance liquid chromatographic method for the determination of the potato glycoalkaloids, alpha-solanine and alpha-chaconine, based on the chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) has been developed. The calibration graph was linear in the range of 5 ng/ml-10 microg/ml for both alpha-solanine and alpha-chaconine. The detection limits of alpha-solanine and alpha-chaconine were 1.2 and 1.3 ng/ml, respectively. This method was successfully applied to a potato tuber sample without cleanup, pre-concentration, and derivatization steps. The recoveries (mean +/- standard deviation, %) of alpha-solanine and alpha-chaconine spiked in tuber pith at 10 microg/g (n = 6) were 101.0 +/- 4.4% and 103.6 +/- 7.1%, respectively. PMID:16194544

  3. Feasibility study for rocket ozone measurements in the 50 to 80 km region using a chemiluminescent technique

    NASA Technical Reports Server (NTRS)

    Goodman, P.

    1973-01-01

    A study has been conducted to determine the feasibility of increasing sensitivity for ozone detection. The detection technique employed is the chemiluminescent reaction of ozone with a rhodamine-B impregnated disk. Previously achieved sensitivities are required to be increased by a factor of about 20 to permit measurements at altitudes of 80 km. Sensitivity was increased by using a more sensitive photomultiplier tube, by increasing the gas velocity past the disk, by different disk preparation techniques, and by using reflective coatings in the disk chamber and on the uncoated side of the glass disk. Reflective coatings provided the largest sensitivity increase. The sum of all these changes was a sensitivity increased by an estimated factor of 70, more than sufficient to permit measurement of ambient ozone concentrations at altitudes of 80 km.

  4. Chemiluminescence-based detection and comparison of protein amounts adsorbed on differently modified silica surfaces.

    PubMed

    Müller, Rainer; Hiller, Karl-Anton; Schmalz, Gottfried; Ruhl, Stefan

    2006-12-15

    The biological consequences of protein adsorption on biomaterial surfaces are considered to be of utmost importance for their biocompatibility. A new method based on amino group-labeling coupled to a chemiluminescence reaction for direct determination of proteins adsorbed on material surfaces was employed. This method was used to explore the effects of surface chemistry and surface roughness on protein adsorption in a silicon oxide model system. Corundum sandblasting was applied to silicon wafers to create roughened surfaces while immobilization of fluorocarbon-, hydrocarbon-, and poly(ethylene glycol)-containing silanes produced surfaces of varying wettability. The adsorption behavior of two complex body fluids, human serum and saliva, and of two purified components, human serum albumin and fibronectin, was strongly influenced by the surface parameters. A general tendency to higher amounts of adsorbed protein was found on roughened surfaces and modification with poly(ethylene glycol) or with fluorocarbon moieties reduced protein adsorption. The values obtained with the new method could be confirmed by a colorimetric determination of protein amounts adsorbed on identically modified silica beads and were in accordance with those previously reported utilizing established methods for protein quantification. The presented method, which was methodically simple to perform and allowed the simultaneous measurement of a large number of samples, may be of future value for high-throughput surveying of the protein adsorption characteristics of biomaterials. PMID:17087913

  5. Automated, high performance, flow-through chemiluminescence microarray for the multiplexed detection of phycotoxins.

    PubMed

    Szkola, Agathe; Campbell, Katrina; Elliott, Christopher T; Niessner, Reinhard; Seidel, Michael

    2013-07-17

    A novel multiplexed immunoassay for the analysis of phycotoxins in shellfish samples has been developed. Therefore, a regenerable chemiluminescence (CL) microarray was established which is able to analyze automatically three different phycotoxins (domoic acid (DA), okadaic acid (OA) and saxitoxin (STX)) in parallel on the analysis platform MCR3. As a test format an indirect competitive immunoassay format was applied. These phycotoxins were directly immobilized on an epoxy-activated PEG chip surface. The parallel analysis was enabled by the simultaneous addition of all analytes and specific antibodies on one microarray chip. After the competitive reaction, the CL signal was recorded by a CCD camera. Due to the ability to regenerate the toxin microarray, internal calibrations of phycotoxins in parallel were performed using the same microarray chip, which was suitable for 25 consecutive measurements. For the three target phycotoxins multi-analyte calibration curves were generated. In extracted shellfish matrix, the determined LODs for DA, OA and STX with values of 0.5±0.3 ?g L(-1), 1.0±0.6 ?g L(-1), and 0.4±0.2 ?g L(-1) were slightly lower than in PBS buffer. For determination of toxin recoveries, the observed signal loss in the regeneration was corrected. After applying mathematical corrections spiked shellfish samples were quantified with recoveries for DA, OA, and STX of 86.2%, 102.5%, and 61.6%, respectively, in 20 min. This is the first demonstration of an antibody based phycotoxin microarray. PMID:23830441

  6. Quantification of carnosine-related peptides by microchip electrophoresis with chemiluminescence detection.

    PubMed

    Zhao, Shulin; Huang, Yong; Shi, Ming; Huang, Junming; Liu, Yi-Ming

    2009-10-01

    A microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of carnosine-related peptides, including carnosine, homocarnosine, and anserine, in biological samples. A simple integrated MCE-CL system was built to perform the assays. The highly sensitive CL detection was achieved by means of the CL reaction between hydrogen peroxide and N-(4-aminobutyl)-N-ethylisoluminol-tagged peptides in the presence of adenine as a CL enhancer and Co(2+) as a catalyst. Experimental conditions for analyte labeling, MCE separation, and CL detection were studied. MCE separation of the above-mentioned three peptides took less than 120 s. Detection limits (signal/noise ratio [S/N]=3) of 3.0x10(-8), 2.8x10(-8), and 3.4x10(-8) M were obtained for carnosine, anserine, and homocarnosine, respectively. The current MCE-CL method was applied for the determination of carnosine, anserine, and homocarnosine in human cerebrospinal fluid (CSF) and canine plasma. Homocarnosine was detected at the micromolar (microM) level in the CSF samples analyzed, whereas the levels of carnosine and anserine in these samples were below the detection limit of the assay. Interestingly, both carnosine and anserine were detected in the canine plasma samples, whereas homocarnosine was not. PMID:19538931

  7. Silver nanoparticles enhanced flow injection chemiluminescence determination of gatifloxacin in pharmaceutical formulation and spiked urine sample.

    PubMed

    Wabaidur, Saikh Mohammad; Alam, Seikh Mafiz; Alothman, Zeid A; Mohsin, Kazi

    2015-06-01

    Silver nanoparticles have been utilized for the enhanced chemiluminogenic estimation of fluoroquinolone antibiotic gatifloxacin. It has been found that the weak chemiluminescence intensity produced from the reaction between calcein and KMnO4 can further be strengthened by the addition of silver nanoparticles in the presence of gatifloxacin. This phenomenon has been exploited to the quantitative determination of gatifloxacin. Under the optimum experimental conditions, the calibration curves are linear over the range of 8.9×10(-9)-4.0×10(-6)M, while the limits of detections were found to be 2.6×10(-9)M with correlation coefficient value (r(2)) 0.9999. The relative standard deviation calculated from six replicate measurements (1.0×10(-4)M gatifloxacin) was 1.70%. The method was applied to pharmaceutical preparations and the results obtained were in reasonable agreement with the amount labeled on the formulations. The proposed method was also used for the determination of gatifloxacin in spiked urine samples with satisfactory results. No interference effects from some common excipients used in pharmaceutical preparations have been found. PMID:25754393

  8. Simultaneous chemiluminescence determination of thebaine and noscapine using support vector machine regression

    NASA Astrophysics Data System (ADS)

    Ensafi, Ali A.; Hasanpour, F.; Khayamian, T.; Mokhtari, A.; Taei, M.

    2010-02-01

    In this work, a batch chemiluminescence (CL) method has been proposed for the simultaneous determination of two structurally similar alkaloids, noscapine and thebaine. The method is based on the kinetic distinction of the CL reactions of noscapine and thebaine with Ru(bipy) 32+ and Ce(IV) system in a sulfuric acid medium. The least squared support vector machine (LS-SVM) regression was applied for relating the concentrations of both compounds to their CL profiles. The parameters of the model consisting of ?2 and ? were optimized by constructing LS-SVM models with all possible combinations of these two parameters to select the model with the minimum root mean squared error of cross validation (RMSECV) as the best. The parameters of this model were then selected as optimized values. Under the optimized experimental conditions for both compounds, the detection limits obtained using the LS-SVM regression were 0.08 and 0.1 ?mol L -1 for noscapine and thebaine, respectively. The proposed method was utilized for the simultaneous determination of the compounds in pharmaceutical formulations and plasma samples with satisfactory results.

  9. Copy Number Variation Analysis by Ligation-Dependent PCR Based on Magnetic Nanoparticles and Chemiluminescence

    PubMed Central

    Liu, Ming; Hu, Ping; Zhang, Gen; Zeng, Yu; Yang, Haowen; Fan, Jing; Jin, Lian; Liu, Hongna; Deng, Yan; Li, Song; Zeng, Xin; Elingarami, Sauli; He, Nongyue

    2015-01-01

    A novel system for copy number variation (CNV) analysis was developed in the present study using a combination of magnetic separation and chemiluminescence (CL) detection technique. The amino-modified probes were firstly immobilized onto carboxylated magnetic nanoparticles (MNPs) and then hybridized with biotin-dUTP products, followed by amplification with ligation-dependent polymerase chain reaction (PCR). After streptavidin-modified alkaline phosphatase (STV-AP) bonding and magnetic separation, the CL signals were then detected. Results showed that the quantification of PCR products could be reflected by CL signal values. Under optimum conditions, the CL system was characterized for quantitative analysis and the CL intensity exhibited a linear correlation with logarithm of the target concentration. To validate the methodology, copy numbers of six genes from the human genome were detected. To compare the detection accuracy, multiplex ligation-dependent probe amplification (MLPA) and MNPs-CL detection were performed. Overall, there were two discrepancies by MLPA analysis, while only one by MNPs-CL detection. This research demonstrated that the novel MNPs-CL system is a useful analytical tool which shows simple, sensitive, and specific characters which are suitable for CNV analysis. Moreover, this system should be improved further and its application in the genome variation detection of various diseases is currently under further investigation. PMID:25553099

  10. Synthesis of Chemiluminescent Esters: A Combinatorial Synthesis Experiment for Organic Chemistry Students

    ERIC Educational Resources Information Center

    Duarte, Robert; Nielson, Janne T.; Dragojlovic, Veljko

    2004-01-01

    A group of techniques aimed at synthesizing a large number of structurally diverse compounds is called combinatorial synthesis. Synthesis of chemiluminescence esters using parallel combinatorial synthesis and mix-and-split combinatorial synthesis is experimented.

  11. Chemiluminescence. 1977-April 1980 (citations from the NTIS Data Base). Report for 1977-April 1980

    SciTech Connect

    Young, C.G.

    1980-05-01

    Cited works describe the use of chemiluminescence for potential chemical laser candidate analyses, combustion studies, measurements of atmospheric properties, and related technical studies. Mention is also made of some biological sources, such as fireflies. (Contains 181 citations)

  12. Stress chemiluminescence of polymeric materials; predictive applications to the aging process

    SciTech Connect

    Monaco, S.B.; Richardson, J.H.; Breshears, J.D.; Lanning, S.M.; Bowman, J.E.; Walkup, C.M.

    1982-01-01

    A computer-controlled stress chemiluminescence instrument has been designed and assembled. The significant result of this work is the correlation of an enhanced chemiluminescence signal in a low stress environment with the subsequent premature mechanical failure of samples of TGMDA-DDS, the most common epoxy system used as a matrix for high-performance composites. Preliminary results with cellular silicone elastomers indicate a correlation between chemiluminescence, a dynamic measurement of a microscopic process, and load deflection curves, a dynamic measurement of macroscopic properties of the elastomer. Arrhenius plots of stressed and unstressed samples yield different activation energies and show a break in the slope at a temperature above which accelerated aging tests become unrealistic. Currently, only epoxy and cellular silicone samples have been examined with any depth; not all epoxy samples give a stress chemiluminescence signal.

  13. Energy distribution among reaction products. VII - H + F2.

    NASA Technical Reports Server (NTRS)

    Polanyi, J. C.; Sloan, J. J.

    1972-01-01

    The 'arrested relaxation' variant of the IR chemiluminescence technique is used in a study of the distribution of vibrational, rotational and translational energies between the products of the reaction by which H + F2 yields HF + F. Diagrams are plotted and numerical values are obtained for the energy distribution rate constants.

  14. Electrochemiluminescence polymerase chain reaction detection of genetically modified organisms

    Microsoft Academic Search

    Jinfeng Liu; Da Xing; Xingyan Shen; Debin Zhu

    2005-01-01

    With the development of biotechnology, more and more genetically modified organisms (GMOs) have entered commercial market. Because of the safety concerns, detection and characterization of GMOs have attracted much attention recently. Electrochemiluminescence (ECL) method is a chemiluminescent (CL) reaction of species generated electrochemically on an electrode surface. It is a highly efficient and accurate detection method. In this paper, ECL

  15. Chemiluminescence from the decomposition of peroxymonocarbonate catalyzed by gold nanoparticles.

    PubMed

    Lin, Jin-Ming; Liu, Meilin

    2008-07-01

    In this work, an online preparation of peroxymonocarbonate was formed innovatively, which offered the reliable intermediate for further investigation. Gold colloids with nanoparticles of different sizes were found to enhance the chemiluminescence (CL) of the peroxymonocarbonate-eosin Y system, and the most intensive CL signals were obtained with 50 +/- 1 nm diameter gold nanoparticles. UV-visible adsorption spectra, fluorescence spectra, transmission electron microscopy images, electron paramagnetic resonance spin-trapping spectra, and mass spectra were obtained in order to study the CL enhancement mechanism. Peroxymonocarbonate, a reactive oxygen species, can be decomposed to singlet oxygen which transfers its energy to eosin Y. The CL can be induced by excited eosin Y. Gold nanoparticles facilitated the radical generation and singlet oxygen molecular formation on the surface of the gold nanoparticles. Thus, the CL emission enhanced greatly by adding gold nanoparticles into the system. PMID:18543872

  16. Polycarbazole-based organic photodiodes for highly sensitive chemiluminescent immunoassays.

    PubMed

    Pires, Nuno M M; Dong, Tao

    2013-01-01

    It is reported the development of a polycarbazole-based organic photodetector for chemiluminescent immunoassays. The optical detector comprised a 1?4 blend by weight of poly [N-9'-heptadecanyl-2,7-carbazole-alt-5,5-(4',7'-di-2-thienyl-2',1',3'-benzothiadiazole)] (PCDTBT) and [6,6]-phenyl C71-butyric acid methyl ester (PC70BM). Optimization of the photodetector design was conducted aiming to maximize photosensitivity and reduce the background level. Quantitation of recombinant human thyroid stimulating hormone indicated good linearity and yielded a detection sensitivity of ?3.7 nA × nM(-1) and a detection limit of 80 pg/ml. PMID:24110033

  17. Electrochemistry, spectroscopy, and electrogenerated chemiluminescence of silole-based chromophores.

    PubMed

    Sartin, Matthew M; Boydston, Andrew J; Pagenkopf, Brian L; Bard, Allen J

    2006-08-01

    We studied the electrochemical and spectroscopic properties of a series of extended silole-based chromophores to understand the effect of structure on behavior. By changing the substituents attached to the chromophore, we observed large variations in luminescence quantum efficiency (ca. 0-0.6), lambdamax for absorbance and photoluminescence (PL), and radical ion stability. The differences are related to the motion in the 2,5-substituents and the steric protection of both the chromophore and the reactive parts of the substituents. For several compounds the electrogenerated chemiluminescence (ECL) spectrum was also compared to the photoluminescence spectrum. In all cases, the ECL lambdamax and the PL lambdamax were about the same. PMID:16881646

  18. Method for detecting pollutants. [through chemical reactions and heat treatment

    NASA Technical Reports Server (NTRS)

    Rogowski, R. S.; Richards, R. R.; Conway, E. J. (inventors)

    1976-01-01

    A method is described for detecting and measuring trace amounts of pollutants of the group consisting of ozone, nitrogen dioxide, and carbon monoxide in a gaseous environment. A sample organic solid material that will undergo a chemical reaction with the test pollutant is exposed to the test environment and thereafter, when heated in the temperature range of 100-200 C., undergoes chemiluminescence that is measured and recorded as a function of concentration of the test pollutant. The chemiluminescence of the solid organic material is specific to the pollutant being tested.

  19. Determination of total sulfur content via sulfur-specific chemiluminescence detection

    SciTech Connect

    Kubala, S.W.; Campbell, D.N. [Fluid Data, Inc., Angleton, TX (United States); DiSanzo, F.P. [Paulsboro Research Lab., NJ (United States)

    1995-12-31

    A specially designed system, based upon sulfur-specific chemiluminescence detection (SSCD), was developed to permit the determination of total sulfur content in a variety of samples. This type of detection system possesses several advantages such as excellent linearity and selectivity, low minimum detectable levels, and an equimolar response to various sulfur compounds. This paper will focus on the design and application of a sulfur-specific chemiluminescence detection system for use in determining total sulfur content in gasoline.

  20. Chemiluminescent Analysis of Gene Expression on High-density Filter Arrays

    Microsoft Academic Search

    Mangalathu S. Rajeevan; Irina M. Dimulescu; Elizabeth R. Unger; Suzanne D. Vernon

    SUMMARY We have optimized conditions for the chemiluminescent analysis of gene ex- pression using high-density filter arrays (HDFAs). High sensitivity and specificity were achieved by optimizing cDNA probe synthesis, hybridization, and detection parameters. The chemiluminescent expression profile reflected expected differences in the transcripts isolated from different sources (placenta and keratinocytes). We estimated the detection limit for low-abundance message to be

  1. Supplementary Information Tandiono et al. 10.1073/pnas.1019623108

    E-print Network

    Ohl, Claus-Dieter

    with the photomultiplier for (a) luminol chemiluminescence and (b) sonoluminescence. The resolution is limited the resolution of the measured FWHM. Therefore, the actual width of the chemiluminescence and sonolumi- nescence (chemiluminescence from Luminol). The field of view is 21 mm × 18 mm. Both pictures were captured with an intensified

  2. Optical tomography of fluorophores in dense scattering media based on ultrasound-enhanced chemiluminescence

    NASA Astrophysics Data System (ADS)

    Kobayashi, Masaki; Kikuchi, Naoto; Sato, Akihiro

    2015-01-01

    This letter proposes and demonstrates ultrasound-combined optical imaging in dense scattering media. A peroxyoxalate chemiluminescence system that includes fluorophores to chemically excite the pigment is stimulated by ultrasound irradiation with power of less than 0.14 W/cm2. Using focused ultrasound, the chemiluminescence is selectively spatially enhanced, which leads to imaging of the pigment when embedded in a light-scattering medium via scanning of the focal point. The ultrasonically enhanced intensity of the chemiluminescence depends on the base intensity of the chemiluminescence without the applied ultrasound irradiation, which thereby enables quantitative determination of the fluorophore concentration. The authors demonstrate the potential of this method to resolve chemiluminescent targets in a dense scattering medium that is comparable to biological tissue. An image was acquired of a chemiluminescent target that included indocyanine green as the fluorophore embedded at a depth of 20 mm in an Intralipid-10% 200 ml/l solution scattering medium (the reduced scattering coefficient was estimated to be approximately 1.3 mm-1), indicating the potential for expansion of this technique for use in biological applications.

  3. Reaction of fluorine and chlorine atoms with formaldehyde and deutero-formaldehyde

    SciTech Connect

    Nogar, N.S.; Fasano, D.M.

    1983-01-01

    The following reactions were studied by using infrared multiple photon dissociation to produce fluorine atoms, and infrared chemiluminescence to monitor the rate of reaction: Cl + H/sub 2/O ..-->.. HCl + HCO; F + H/sub 2/CO ..-->.. HF + HCO; Cl + D/sub 2/CO ..-->.. DCl + DCO; and F + D/sub 2/CO ..-->.. DF + DCO.

  4. Real time imaging of live cell ATP leaking or release events by chemiluminescence microscopy

    SciTech Connect

    Zhang, Yun

    2008-12-18

    The purpose of this research was to expand the chemiluminescence microscopy applications in live bacterial/mammalian cell imaging and to improve the detection sensitivity for ATP leaking or release events. We first demonstrated that chemiluminescence (CL) imaging can be used to interrogate single bacterial cells. While using a luminometer allows detecting ATP from cell lysate extracted from at least 10 bacterial cells, all previous cell CL detection never reached this sensitivity of single bacteria level. We approached this goal with a different strategy from before: instead of breaking bacterial cell membrane and trying to capture the transiently diluted ATP with the firefly luciferase CL assay, we introduced the firefly luciferase enzyme into bacteria using the modern genetic techniques and placed the CL reaction substrate D-luciferin outside the cells. By damaging the cell membrane with various antibacterial drugs including antibiotics such as Penicillins and bacteriophages, the D-luciferin molecules diffused inside the cell and initiated the reaction that produces CL light. As firefly luciferases are large protein molecules which are retained within the cells before the total rupture and intracellular ATP concentration is high at the millmolar level, the CL reaction of firefly luciferase, ATP and D-luciferin can be kept for a relatively long time within the cells acting as a reaction container to generate enough photons for detection by the extremely sensitive intensified charge coupled device (ICCD) camera. The result was inspiring as various single bacterium lysis and leakage events were monitored with 10-s temporal resolution movies. We also found a new way of enhancing diffusion D-luciferin into cells by dehydrating the bacteria. Then we started with this novel single bacterial CL imaging technique, and applied it for quantifying gene expression levels from individual bacterial cells. Previous published result in single cell gene expression quantification mainly used a fluorescence method; CL detection is limited because of the difficulty to introduce enough D-luciferin molecules. Since dehydration could easily cause proper size holes in bacterial cell membranes and facilitate D-luciferin diffusion, we used this method and recorded CL from individual cells each hour after induction. The CL light intensity from each individual cell was integrated and gene expression levels of two strain types were compared. Based on our calculation, the overall sensitivity of our system is already approaching the single enzyme level. The median enzyme number inside a single bacterium from the higher expression strain after 2 hours induction was quantified to be about 550 molecules. Finally we imaged ATP release from astrocyte cells. Upon mechanical stimulation, astrocyte cells respond by increasing intracellular Ca{sup 2+} level and releasing ATP to extracellular spaces as signaling molecules. The ATP release imaged by direct CL imaging using free firefly luciferase and D-luciferin outside cells reflects the transient release as well as rapid ATP diffusion. Therefore ATP release detection at the cell surface is critical to study the ATP release mechanism and signaling propagation pathway. We realized this cell surface localized ATP release imaging detection by immobilizing firefly luciferase to streptavidin beads that attached to the cell surface via streptavidin-biotin interactions. Both intracellular Ca{sup 2+} propagation wave and extracellular ATP propagation wave at the cell surface were recorded with fluorescence and CL respectively. The results imply that at close distances from the stimulation center (<120 {micro}m) extracellular ATP pathway is faster, while at long distances (>120 {micro}m) intracellular Ca{sup 2+} signaling through gap junctions seems more effective.

  5. Development and optimization of an integrated capillary-based opto-microfluidic device for chemiluminescence quantitative detection

    NASA Astrophysics Data System (ADS)

    Honrado, Carlos; Dong, Tao

    2014-12-01

    A capillary-action driven device amenable for integration of organic photodiodes (OPDs) was developed for monitoring parallel chemiluminescence (CL) reactions. Device characterization was conducted using finite element method (FEM) simulations. Definition of the simulation setup, dimensional optimization of the reaction chamber and overall geometrical characterization of the microfluidic device were the main simulation results. Furthermore, a non-uniform filling process was observed during the final simulation of the capillary device. Validation of this result and the proposed capillary-driven filling process was later confirmed by experimental results. Experimental testing performed on a single chamber defined an optimal exposure time to the luminescent substrate of 5?min, indicating a quick analyte detection time. Further tests using one chamber presented a linear relation between the signal-to-noise ratio and increasing concentrations of the protein used. A measured limit of detection of 28?nM was obtained for streptavidin. Regarding the tests performed on the whole device, acceptable values of 39?s ± 5?s were obtained for the luminescent substrate total filling times. Also, the microfluidic device showed the capability to perform a quantitative detection of the occurring CL reactions. Weaker optical signals, due to the occurrence of CL reactions, were detected in the chambers with a later filling process, as predicted by simulation results. Notwithstanding these results, the capillary-based device is promising for quantitative detection of proteins in future point-of-care systems, presenting an unprompted filling process and parallel quantitative detection capability.

  6. The Oxidant-Scavenging Abilities in the Oral Cavity May Be Regulated by a Collaboration among Antioxidants in Saliva, Microorganisms, Blood Cells and Polyphenols: A Chemiluminescence-Based Study

    PubMed Central

    Ginsburg, Isaac; Kohen, Ron; Shalish, Miri; Varon, David; Shai, Ella; Koren, Erez

    2013-01-01

    Saliva has become a central research issue in oral physiology and pathology. Over the evolution, the oral cavity has evolved the antioxidants uric acid, ascorbate reduced glutathione, plasma-derived albumin and antioxidants polyphenols from nutrients that are delivered to the oral cavity. However, blood cells extravasated from injured capillaries in gingival pathologies, or following tooth brushing and use of tooth picks, may attenuate the toxic activities of H2O2 generated by oral streptococci and by oxidants generated by activated phagocytes. Employing a highly sensitive luminol-dependent chemiluminescence, the DPPH radical and XTT assays to quantify oxidant-scavenging abilities (OSA), we show that saliva can strongly decompose both oxygen and nitrogen species. However, lipophilic antioxidant polyphenols in plants, which are poorly soluble in water and therefore not fully available as effective antioxidants, can nevertheless be solubilized either by small amounts of ethanol, whole saliva or also by salivary albumin and mucin. Plant-derived polyphenols can also act in collaboration with whole saliva, human red blood cells, platelets, and also with catalase-positive microorganisms to decompose reactive oxygen species (ROS). Furthermore, polyphenols from nutrient can avidly adhere to mucosal surfaces, are retained there for long periods and may function as a “slow- release devises” capable of affecting the redox status in the oral cavity. The OSA of saliva is due to the sum result of low molecular weight antioxidants, albumin, polyphenols from nutrients, blood elements and microbial antioxidants. Taken together, saliva and its antioxidants are considered regulators of the redox status in the oral cavity under physiological and pathological conditions. PMID:23658797

  7. Chemiluminescent Diagnostics of Free-Radical Processes in an Abiotic System and in Liver Cells in the Presence of Nanoparticles Based on Rare-Earth Elements nReVO4:Eu3+ (Re = Gd, Y, La) and CeO2

    NASA Astrophysics Data System (ADS)

    Averchenko, E. A.; Kavok, N. S.; Klochkov, V. K.; Malyukin, Yu. V.

    2014-11-01

    We have used luminol-dependent chemiluminescence with Fenton's reagent to study the effect of nanoparticles based on rare-earth elements of different sizes and shapes on free-radical processes in abiotic and biotic cell-free systems, and also in isolated cells in vitro. We have estimated the effects of rare-earth orthovanadate nanoparticles of spherical (GdYVO4:Eu3+, 1-2 nm), spindle-shaped (GdVO4:Eu3+, 25 ×8 nm), and rod-shaped (LaVO4:Eu3+, 57 × (6-8) nm) nanoparticles and spherical CeO2 nanoparticles (sizes 1-2 nm and 8-10 nm). We have shown that in contrast to the abiotic system, in which all types of nanoparticles exhibit antiradical activity, in the presence of biological material, extra-small spherical (1-2 nm) nanoparticles of both types exhibit pro-oxidant activity, and also enhance pro-oxidant induced oxidative stress (for the pro-oxidants hydrogen peroxide and tert-butyl hydroperoxide). The effect of rare-earth orthovanadate spindle and rod shaped nanoparticles in this system was neutral; a moderate antioxidant effect was exhibited by 8-10 nm CeO2 nanoparticles.

  8. Development of a multichannel Fourier-transform spectrometer to measure weak chemiluminescence: Application to the emission of singlet-oxygen dimol in the decomposition of hydrogen peroxide with gallic acid and K 3[Fe(CN) 6

    NASA Astrophysics Data System (ADS)

    Tsukino, Kazuo; Satoh, Toshihiro; Ishii, Hiroshi; Nakata, Munetaka

    2008-05-01

    A Fourier-transform spectrometer equipped with a Savart-plate polarization interferometer was developed for observation of weak chemiluminescence and applied to a measurement of emission spectra in the decomposition of hydrogen peroxide with gallic acid and K 3[Fe(CN) 6]. The band appearing at ˜580 nm in the chemiluminescence spectrum was assigned to the emission of singlet-oxygen dimol, the peak wavelength being shifted from that observed in the reaction of hydrogen peroxide with sodium hypochlorite, ˜633 nm. The band intensity was increased with the increasing concentration of K 3[Fe(CN) 6] up to ˜100 mM, and thereafter the peak wavelength was shifted from 580 to 700 nm with a decrease in the intensity.

  9. Gas-phase chemiluminescence with ozone oxidation for the determination of total tin in environmental samples using flow injection hydride generation and cryotrapping.

    PubMed

    Ye, Yousheng; Sang, Jianchi; Ma, Hongbing; Tao, Guanhong

    2010-09-16

    The gas-phase chemiluminescence detection system based on the ozonization of gaseous hydride was exploited and utilized for the determination of total tin in environmental samples. After sample treatment, tin was reduced to stannane by sodium borohydride in a phosphate buffer medium of pH 5.8. Flow injection technique was used to control the reaction precisely and reproducibly. The generated stannane, carried by helium, was separated from liquid and dried using an ice-salt cryogenic bath and subsequently trapped in a glass U-tube immersed in liquid nitrogen. The cryotrapped stannane was vaporized by bringing the tube to room temperature and subsequently carried in a flow of helium to a reaction chamber where ozone was used to oxidize the stannane. Chemiluminescence was produced during the oxidation process, which was utilized for the measurement of tin. Under optimal conditions, a detection limit of 0.32 ?g L(-1) was achieved with a relative standard deviation of 3.1% (10.0 ?g L(-1) Sn, n=11). Interferences from transition metal ions and other hydride forming elements were reduced by the addition of 1,10-phenanthroline and through optimized hydride generation conditions. The proposed system was applied to the determination of Sn in water and soil samples with satisfactory results. PMID:20837181

  10. Performances of amorphous silicon photodiodes integrated in chemiluminescence based ?-TAS

    NASA Astrophysics Data System (ADS)

    Caputo, Domenico; Nardini, Massimo; Scipinotti, Riccardo; de Cesare, Giampiero; Mirasoli, Mara; Zangheri, Martina; Roda, Aldo; Nascetti, Augusto

    2013-05-01

    A detailed characterization of the performances of amorphous silicon photodiodes in the detection of chemiluminescent signal is carried out. Comparison with commercial CCD acquisition system has been done as benchmark. The underlying idea is the development of stand-alone and compact micro-total-analysys-systems (?-TAS) that do not need bulky and expensive equipment for their operation as external focusing optics and excitation sources. The photosensor is p-i-n structures deposited by Plasma Enhanced Chemical Vapour Deposition on a glass substrate covered with a transparent conductive oxide that acts as bottom electrode and window layer for the light impinging through the glass. A PDMS layer with wells has been fabricated using an aluminum mold and bonded on the glass substrate with a well aligned with a photosensor. The experiments have been performed by filling a well with solutions containing different quantities of horseradish peroxidase. A good linearity of the photosensor response is observed across the entire measurement range that spans over three orders of magnitude. The system detection limit is 70 fg/?L. A very good agreement between results achieved with conventional off-chip CCD detection and the on-chip photodiode has been observed. Experiments with target molecules immobilized on a functionalized glass surface have been also performed in microfluidic regime, confirming the validity of the proposed integrated approach based on a-Si:H technology.

  11. Sensitive determination of triacetone triperoxide explosives using electrogenerated chemiluminescence.

    PubMed

    Parajuli, Suman; Miao, Wujian

    2013-08-20

    Sensitive and selective detection and quantification of high explosive triacetone triperoxide (TATP) with electrogenerated chemiluminescence (ECL) at a glassy carbon electrode in water-acetonitrile solvent mixture were reported. In the presence of ruthenium(II) tris(bipyridine), TATP or hydrogen peroxide derived from TATP via UV irradiation or acid treatment produced ECL emissions upon cathodic potential scanning. Interference from hydrogen peroxide on TATP detection was eliminated by pretreatment of the analyte with catalase enzyme. Selective detection of TATP from hexamethylene triperoxide diamine (HMTD, another common peroxide-based explosive) was realized by comparing ECL responses obtained from the anodic and the cathodic potential scanning; TATP produced ECL upon cathodic potential scanning only, whereas HMTD produced ECL upon both cathodic and anodic potential scanning. The hydroxyl radical formed after the electrochemical reduction of TATP was believed to be the key intermediate for ECL production, and its stability was strongly dependent on the solution composition, which was verified with electron paramagnetic resonance spectroscopy. A detection limit of 2.5 ?M TATP was obtained from direct electrochemical reduction of the explosive or hydrogen peroxide derived from TATP in 70/30% (v/v) water-acetonitrile solutions, which was ~400 times lower than that reported previously based on liquid chromatography separation and Fourier transform infrared detection. PMID:23885721

  12. Evaluation of a new chemiluminescence immunoassay for diagnosis of syphilis

    PubMed Central

    2010-01-01

    Objective To assess the sensitivity, specificity, and feasibility of a new chemiluminescence immunoassay (CLIA) in the diagnosis of syphilis. Methods At first, a retrospective study was conducted, using 135 documented cases of syphilis and 30 potentially interfering samples and 80 normal sera. A prospective study was also performed by testing 2, 071 unselected samples for routine screening for syphilis. CLIA was compared with a nontreponemal test (TRUST) and a treponemal test (TPPA). Results There was an agreement of 100% between CLIA and TPPA in the respective study. The percentage of agreement among the 245 sera tested was 100.0%. Compared with TPPA, the specificity of CLIA was 99.9% (1817/1819), the sensitivity of CLIA was 100.0% (244/244) in the prospective study. CLIA showed 99.5% agreement with TPPA by testing 2, 071 unselected samples. And CLIA seemed to be more sensitive than TPPA in detecting the samples of primary syphilis. Conclusions CLIA is easy to perform and the indicator results are objective and unequivocal. It may be suitable for large-scale screening as a treponemal test substituted for TPPA. PMID:20452886

  13. A 22-plex chemiluminescent microarray for pneumococcal antibodies.

    PubMed

    Pickering, Jerry W; Hoopes, Justin D; Groll, Matthew C; Romero, Heidi K; Wall, Dave; Sant, Howard; Astill, Mark E; Hill, Harry R

    2007-07-01

    We developed a chemiluminescent multiplexed microarray that simultaneously determines IgG antibody concentrations to 22 pneumococcal polysaccharide (PnPs) serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 23F, and 33F). We compared the microarray with an enzyme-linked immunosorbent assay (ELISA) for 9 of the 22 serotypes (1, 4, 5, 6B, 9V, 14, 18C, 19F, and 23F). Correlation coefficients (r2) for the comparison of the microarray with ELISA ranged from 0.91 to 0.97 for the 9 serotypes. The microarray detected more than 4-fold increases in antibody concentrations in serum samples from before and 1 month after administration of pneumococcal vaccine for all 22 serotypes tested. The mean interassay and intra-assay coefficients of variation for 12 serum samples for the 22 serotypes were 7.6% and 6.0%, respectively. Inhibition-of-binding studies showed more than 90% inhibition by homologous serotypes and, with few exceptions, less than 25% inhibition by heterologous serotypes. The microarray multiplexing technology is an attractive alternative to ELISA for antibody responses to 23-valent PnPs vaccines. PMID:17580269

  14. Determination of nitrate in atmospheric particulate matter by thermal decomposition and chemiluminescence

    SciTech Connect

    Spicer, C.W.; Joseph, D.W.; Schumacher, P.M.

    1985-10-01

    A thermal decomposition/chemiluminescence method is presented for determining nitrate in atmospheric particular matter. Nitrate in the sample is thermally decomposed to NO/sub x/, which is then determined with a commercial chemiluminescence NO/sub x/ monitor. The nitrate in a filter sample can be determined directly by heating a segment of the filter in a furnace or after extraction of the filter by flash heating the aqueous extract in a sample loop. In either case, the sample is decomposed in a nitrogen atmosphere to avoid interference from ammonium. The NO/sub x/ peak from nitrate decomposition can be quantified by integrating the chemiluminescence signal or by integrating the gas sample in a Tedlar bag prior to the chemiluminescence measurement. The technique is straightforward, fast, and sensitive, and interferences in atmospheric samples are negligible. A comparison of the thermal decomposition/chemiluminescence method with ion chromatography using filter samples collected in ambient air showed good agreement over a wide range of concentrations. 26 references.

  15. THE JOURNAL OF CHEMICAL PHYSICS VOLUME 57, NUMBER 12 15 DECEMBER 1972 Determination of Doo(AIO) from Crossed-Beam Chemiluminescence of Al +03

    E-print Network

    Zare, Richard N.

    (AIO) from Crossed-Beam Chemiluminescence of Al +03 J. L. GoLE· AND R. N. ZARE Department of Chemistry (3000K) and the resulting visible chemiluminescence is re- corded with aim scanning monochromator. The chemiluminescence spectrum consists of (1) the AlO B 22;+-X 22;+ blue--green system for which we have assigned bands

  16. Chemiluminescent simultaneous determination of phosphatidylcholine hydroperoxide and phosphatidylethanolamine hydroperoxide in the liver and brain of the rat

    Microsoft Academic Search

    Teruo Miyazawa; Toshihide Suzuki; Kenshiro Fujimoto; Keiichi Yasuda

    The quantification of phospholipid hydroperoxides in biological tissues is important in order to know the degree of peroxidative damage of membrane lipids. For this purpose, op- timal conditions for the chemiluminescent simultaneous assay of phosphatidylcholine hydroperoxide (PCOOH) and phos- phatidylethanolamine hydroperoxide (PEOOH) in rat liver and brain were determined. A chemiluminescence detection-high performance liquid chromatography (CL-HPLC) method that incorporates cytochrome

  17. Chemiluminescence. 1977-April 1980, 1980 (citations from the International Aerospace Abstracts data base). Report for 1977-April 1980

    SciTech Connect

    Young, C.G.

    1980-05-01

    Cited works describe the use of chemiluminescence for aurora and upper-atmosphere analyses, evaluation of chemical laser candidates, studies of jet and combustor dynamics, smog analysis and other work. Sources and forms of chemiluminescence and measurement techniques are described. (Contains 92 citations)

  18. A competitive chemiluminescence enzyme immunoassay for rapid and sensitive determination of enrofloxacin

    NASA Astrophysics Data System (ADS)

    Yu, Fei; Wu, Yongjun; Yu, Songcheng; Zhang, Huili; Zhang, Hongquan; Qu, Lingbo; Harrington, Peter de B.

    With alkaline phosphatase (ALP)-adamantane (AMPPD) system as the chemiluminescence (CL) detection system, a highly sensitive, specific and simple competitive chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of enrofloxacin (ENR). The physicochemical parameters, such as the chemiluminescent assay mediums, the dilution buffer of ENR-McAb, the volume of dilution buffer, the monoclonal antibody concentration, the incubation time, and other relevant variables of the immunoassay have been optimized. Under the optimal conditions, the detection linear range of 350-1000 pg/mL and the detection limit of 0.24 ng/mL were provided by the proposed method. The relative standard deviations were less than 15% for both intra and inter-assay precision. This method has been successfully applied to determine ENR in spiked samples with the recovery of 103%-96%. It showed that CLEIA was a good potential method in the analysis of residues of veterinary drugs after treatment of related diseases.

  19. Screening test for rapid food safety evaluation by menadione-catalysed chemiluminescent assay.

    PubMed

    Yamashoji, Shiro; Yoshikawa, Naoko; Kirihara, Masayuki; Tsuneyoshi, Toshihiro

    2013-06-15

    The chemiluminescent assay of menadione-catalysed H2O2 production by living mammalian cells was proposed to be useful for rapid food safety evaluation. The tested foods were extracted with water, ethanol and dimethylsulfoxide, and each extract was incubated with NIH3T3, Neuro-2a and HepG2 cells for 4h. Menadione-catalysed H2O2 production by living mammalian cells exposed to each extract was determined by the chemiluminescent assay requiring only 10 min, and the viability of the cells was estimated as percentage based on H2O2 production by intact cells. In this study the cytotoxicity of food was rated in order of inhibitory effect on H2O2 production by intact cells. The well known natural toxins such as Fusarium mycotoxin, tomato toxin tomatine, potato toxin solanine and marine toxins terodotoxin and brevetoxin could be detected by the above chemiluminescent assay. PMID:23497869

  20. Chemiluminescent and flow cytometric analysis of gamma interferon preincubation on neonatal and adult rat polymorphonuclear leukocytes.

    PubMed Central

    Wittler, R R; Lieberman, M M; Paine, D D; Muehlbauer, S L; Lima, J E; Sachanandani, D M; Pinney, C A

    1996-01-01

    Gamma interferon (IFN-gamma) has multiple immunomodulating effects and has been postulated as a possible immunopotentiating agent for the prevention or treatment of neonatal infections. This report describes the effect of rat recombinant IFN-gamma on the oxidative burst activity and CD11b expression of neonatal and adult rat polymorphonuclear leukocytes (PMNL). Oxidative burst activity was assessed by chemiluminescence and dihydrorhodamine flow cytometry. Neonatal PMNL exhibited significantly less oxidative burst activity than did adult PMNL. IFN-gamma mildly enhanced the chemiluminescence response of PMNL from both the rat pups and adults, but this effect was not statistically significant when analyzed by a multivariate model of repeated-measures analysis of variance for both chemiluminescence and dihydrorhodamine flow cytometry. CD11b expression was also not significantly enhanced by IFN-gamma. PMID:8877130

  1. [Application of chemiluminescent technique for determination of Daphnia magna sensitivity to various types of toxic agents].

    PubMed

    Levkovets', I A; Ivashkevych, S P; Nazarenko, V I; Starodub, M F

    2002-01-01

    The character and intensity of activated chemiluminescence of Daphnia living medium are investigated depending on the effect on them of various concentration different in nature toxic substances, such, as potassium bichromate, methomyl and tween-80. It is shown, that the toxic influence of these agents can be defined by registration of stimulating effect of Daphnia's exometabolites on the process of chemiluminescence. The sensitivity of the method makes: for potassium chromate--0.005 mg/l, methomyl--0.0013 mg/l and tween-80--0.1 mg/l. PMID:12924025

  2. Simultaneous particle image velocimetry and chemiluminescence visualization of millisecond-pulsed current-voltage-induced perturbations of a premixed propane/air flame

    NASA Astrophysics Data System (ADS)

    Schmidt, Jacob; Kostka, Stanislav; Lynch, Amy; Ganguly, Biswa

    2011-09-01

    The effects of millisecond-wide, pulsed current-voltage-induced behavior in premixed laminar flames have been investigated through the simultaneous collection of particle image velocimetry (PIV) and chemiluminescence data with particular attention paid to the onset mechanisms. Disturbances caused by applied voltages of 2 kV over a 30-mm gap to a downward propagating, atmospheric pressure, premixed propane/air flame with a flow speed near 2 m/s and an equivalence ratio of 1.06 are investigated. The combined PIV and chemiluminescence-based experimental data show the observed disturbance originates only in or near the cathode fall region very close to the burner base. The data also suggest that the coupling mechanism responsible for the flame disturbance behavior is fluidic in nature, developing from the radial positive chemi-ion distribution and an ion-drift current-induced net body force that acts along the annular space discharge distribution in the reaction zone in or near the cathode fall. This net body force causes a reduction in flow speed above these near cathodic regions causing the base of the flame to laterally spread. Also, this effect seems to produce a velocity gradient leading to the transition of a laminar flame to turbulent combustion for higher applied current-voltage conditions as shown in previous work (Marcum and Ganguly in Combust Flame 143:27-36, 2005; Schmidt and Ganguly in 48th AIAA aerospace sciences meeting. Orlando, 2010).

  3. Characterization of the oxidant generation by inflammatory cells lavaged from rat lungs following acute exposure to ozone

    SciTech Connect

    Esterline, R.L.; Bassett, D.J.; Trush, M.A.

    1989-06-15

    Following exposure to 2 ppm ozone for 4 hr, two distinct effects on rat lung inflammatory cell oxidant generation were observed. TPA- and opsonized zymosan-stimulated superoxide production by the inflammatory cell population was found to be maximally inhibited 24 hr following ozone exposure. In contrast, luminol-amplified chemiluminescence increased 24 hr following ozone exposure, coinciding with an increase in the percentage of neutrophils and myeloperoxidase in the inflammatory cell population. Supporting the involvement of myeloperoxidase in the enhanced oxidant-generating status of these cells, the luminol-amplified chemiluminescence was found to be azide-, but not superoxide dismutase-inhibitable. Additionally, this cell population was found to generate taurine chloramines, a myeloperoxidase-dependent function which was absent prior to the ozone exposure and also demonstrated enhanced activation of benzo(a)pyrene-7,8-dihydrodiol to its light-emitting dioxetane intermediate. Addition of myeloperoxidase to control alveolar macrophages resulted in enhanced luminol-amplified chemiluminescence, taurine chloramine generation, and enhanced chemiluminescence from benzo(a)pyrene-7,8-dihydrodiol demonstrating that, in the presence of myeloperoxidase, alveolar macrophages are capable of supporting myeloperoxidase-dependent reactions. The possibility of such an interaction occurring in vivo is suggested by the detection of myeloperoxidase activity in the cell-free lavagates of ozone-exposed rats. These studies suggest that neutrophils recruited to ozone-exposed lungs alter the oxidant-generating capabilities in the lung which could further contribute to lung injury or to the metabolism of inhaled xenobiotics.

  4. [Extra-weak chemiluminescence of drugs. XIV. Quenching effects of anthraquinones on the extra-weak chemiluminescence from lipid peroxidation in rat brain homogenates].

    PubMed

    Sato, H; Ishizawa, F; Hirayama, H; Hishinuma, T; Mizugaki, M

    1992-03-01

    Quenching effects of anthraquinones on the extra-weak chemiluminescence (CL) derived from lipid peroxidation in rat brain homogenates were investigated. Such anthraquinone derivatives as emodin, rhein, and alizarin quenched the CL, while anthraquinone did not quench the CL. A linear relationship between CL-quenching activity and inhibitory rate of malondialdehyde production of various compounds was demonstrated. This technique is useful for the screening method of antioxidants. PMID:1608044

  5. Evaluation of the Health Level in Children by the Chemiluminescence Method

    Microsoft Academic Search

    S. A. Kantyukov; L. V. Krivokhizhina; G. P. Ganchenkova

    2001-01-01

    Changes in the free-radical oxidation and accumula- tion of peroxides can affect the physiological functions of the body via alterations in the physicochemical prop- erties of the biological membranes, functional activity of enzymes, the state of nucleic acids, the cell protein spectrum, and the rate of mitochondrial oxidation (5). Currently chemiluminescence methods are the most effective in investigating free-radical oxidation.

  6. Evaluation of a new chemiluminescence immunoassay for laboratory diagnosis of syphilis.

    PubMed

    Sampedro-Martinez, A; Padilla-Malo, A; Gomez-Camarasa, C; Rodriguez-Granger, J; Lara-Oya, A

    2013-08-01

    The purpose of this study was to evaluate diagnostic performances of automated chemiluminescence immunoassay (CLIA) in comparison with Treponema pallidum hemagglutination test (TPHA). The specificity of CLIA was 98.9% and 99.6% for TPHA, whereas the sensitivity was 98% and 96%, respectively. Considering the suitability for automation, CLIA may represent a suitable alternative. PMID:23732753

  7. Chemiluminescence detection in liquid chromatography: Applications to clinical, pharmaceutical, environmental and food analysis—A review

    Microsoft Academic Search

    Laura Gámiz-Gracia; Ana M. García-Campaña; José F. Huertas-Pérez; Francisco J. Lara

    2009-01-01

    Chemiluminescence (CL)-based detection has become in the last years quite a useful detecting tool in liquid chromatography (HPLC) due to its simplicity, low cost and high sensitivity and selectivity, and the development in instrumentation. Minimal instrumentation is required and no external light source is needed; thus, the optical system is quite simple. As a consequence, a wide variety of analytical

  8. Determination of procaine, benzocaine and tetracaine by sequential injection analysis with permanganate-induced chemiluminescence detection

    Microsoft Academic Search

    Hana Paseková; Miroslav Polášek

    2000-01-01

    Local anaesthetics procaine hydrochloride (I), benzocaine (II), and tetracaine hydrochloride (III) were determined by the technique of sequential injection analysis (SIA) with chemiluminescence (CL) detection. The CL was emitted during the oxidation of the analytes by permanganate in aqueous sulphuric acid in the presence of various CL enhancers (4-hydroxybiphenyl, Rhodamine B, glycolaldehyde, glutaraldehyde and formic acid). The optimum enhancer or

  9. Decrease of neutrophils chemiluminescence during exposure to low-power laser infrared radiation

    Microsoft Academic Search

    Zenon P. Czuba; Mariusz Adamek; Wojciech Krol; Aleksander Sieron; Grzegorz Cieslar

    1995-01-01

    The neutrophil is the cell in which phagocyting and transforming of some exogeneous agents results in marked stimulation of nonmitochondrial respiratory chain activity (respiratory burst). In our experiment we focused on determining the level of chemiluminescence (CL) of stimulated neurotrophils during and after irradiation, measuring the photon emission intensity in 6 second's intervals. We used Ga-Al-As pulsed laser (wavelength 904

  10. Electrogenerated Chemiluminescence of Partially Oxidized Highly Oriented Pyrolytic Graphite Surfaces and of Graphene Oxide Nanoparticles

    E-print Network

    Electrogenerated Chemiluminescence of Partially Oxidized Highly Oriented Pyrolytic Graphite electrochemically oxidized highly oriented pyrolytic graphite (HOPG) and from a suspension of graphene oxide, and electrical devices. For example, graphene, a one-atom thick sheet of carbon, and its oxidized form, graphene

  11. Evaluation of endotoxin (LPS) activity in bovine blood using neutrophil dependent chemiluminescence

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The purpose of this study was to evaluate the applicability of a neutrophil chemiluminescence-based assay for the measurement of LPS stimulatory activity in bovine whole blood. The assay is based on the capacity for LPS to trigger the respiratory oxidative burst activity (RBA) of autologous neutroph...

  12. Crossed-beam chemiluminescent studies of alkaline earth atoms with CI02

    E-print Network

    Zare, Richard N.

    Crossed-beam chemiluminescent studies of alkaline earth atoms with CI02 F. Engelke,* R. K. Sander. Although the MCI· product results from attack of the central CI atom in CIO" its cross section processes in gases has a long and colorful history, 1 but only with the ad- vent of beam studies2 under

  13. Fabrication technology of chemiluminescent sensitive elements for rocket-borne ozone detectors

    NASA Astrophysics Data System (ADS)

    Kononkov, V. A.; Lelikova, A. I.; Perov, S. P.

    Attention is given to the technology behind the fabrication of chemiluminescent sensitive elements for rocket-borne ozone detectors. High-silica microporous glass is the basic material required for these detectors. It is noted that the luminophor consists of rhodamine-C and gallic acid, and that the desired ratio of these components depends on the sensitivity of a particular specimen to ozone.

  14. Anal. Chem. 1994,66, 303-306 End-Column Chemiluminescence Detector for Capillary

    E-print Network

    Zare, Richard N.

    Anal. Chem. 1994,66, 303-306 End-Column Chemiluminescence Detector for Capillary Electrophoresis Ra of Chemistry, State University of New York, Buffalo, NY 14214. (1) Jorgenson, J. W.; Lukacs, K. D. Anal. Chem,S. L., Jr.; a r e ,R. N.; Quint,J. Anal. Chem. 1989,61, 1642-1647. (7) Kobayashi, S I . ; Imai, K. Anal

  15. Determination of glyphosate in foodstuff by one novel chemiluminescence-molecular imprinting sensor

    Microsoft Academic Search

    Peini Zhao; Mei Yan; Congcong Zhang; Ruixue Peng; Dongsheng Ma; Jinghua Yu

    2011-01-01

    A novel chemiluminescence (CL) sensor for the determination of glyphosate (GLY) was made up based on molecularly imprinted polymer (MIP). The molecularly imprinted microspheres (MIMs) with a small dimension which possess extremely high surface-to-volume ratio were synthesized using precipitation polymerization with GLY as template. And then the MIMs were modified on glass sheets, which were placed at the bottom of

  16. The Synthesis and Chemiluminescence of a Stable 1,2-Dioxetane.

    ERIC Educational Resources Information Center

    Meijer, E. W.; Wynberg, Hans

    1982-01-01

    Background information, laboratory procedures, and discussion of results are provided for the synthesis and chemiluminescence of adamantylideneadamantane-1,2-dioxetane (I). Results provided were obtained during a normal junior level organic laboratory course. All intermediates and products were identified using routine spectroscopic analysis.…

  17. Chemiluminescence: Measuring methods. (Latest citations from the NTIS bibliographic database). Published Search

    SciTech Connect

    NONE

    1996-05-01

    The bibliography contains citations concerning chemiluminescence assays. The citations include sample system design, sample collection, measurement techniques, and sensitivity of the instrumentation. Applications in high altitude air pollution studies are emphasized. (Contains 50-250 citations and includes a subject term index and title list.) (Copyright NERAC, Inc. 1995)

  18. Chemiluminescence: Measuring methods. (Latest citations from the NTIS data base). Published Search

    SciTech Connect

    Not Available

    1992-10-01

    The bibliography contains citations concerning chemiluminescence assays. The citations include sample system design, sample collection, measurement techniques, and sensitivity of the instrumentation. Applications in high altitude air pollution studies are emphasized. (Contains 250 citations and includes a subject term index and title list.)

  19. Automated Flow-Injection Instrument for Chemiluminescence Detection Using a Low-Cost Photodiode Detector

    ERIC Educational Resources Information Center

    Economou, A.; Papargyris, D.; Stratis, J.

    2004-01-01

    The development of an FI analyzer for chemiluminescence detection using a low-cost photoiodide is presented. The experiment clearly demonstrates in a single interdisciplinary project the way in which different aspects in chemical instrumentation fit together to produce a working analytical system.

  20. Chemiluminescence in the oxidation of uranium (IV) by xenon trioxide and its analytical possibilities

    SciTech Connect

    Khamidullina, L.A.; Lotnik, S.V.; Gusev, Yu.K.; Kazakov, V.P.

    1988-09-01

    This work is devoted to an investigation of the previously detected chemiluminescence in the oxidation of uranium (IV) by xenone trioxide and to evaluating the possibility of using it in determining nanogram quantities of U/sup (IV)/ in solution, including solutions containing a large excess of U/sup (VI)/.

  1. Technical note Synergetic chemiluminescence and label-free dual detection for developing

    E-print Network

    of chemiluminescence detection. © 2011 Elsevier B.V. All rights reserved. Keywords: Hepatitis Immunoassay Microarray). With the existing approach, immunoassays are run separately and add linearly to cost. Furthermore, separate tests immunoassays do not cross-react or otherwise negatively impact one another. In a typical sandwich assay

  2. Laser-saturated fluorescence of nitric oxide and chemiluminescence measurements in premixed ethanol flames

    SciTech Connect

    Marques, Carla S.T.; Barreta, Luiz G.; Sbampato, Maria E.; dos Santos, Alberto M. [Aerothermodynamic and Hypersonic Division, Institute of Advanced Studies - General Command of Aerospatial Technology, Rodovia dos Tamoios, km 5.5, 12228-001 Sao Jose dos Campos - SP (Brazil)

    2010-11-15

    In this study, nitric oxide laser-saturated fluorescence (LSF) measurements were acquired from premixed ethanol flames at atmospheric pressure in a burner. NO-LSF experimental profiles for fuel-rich premixed ethanol flames ({phi} = 1.34 and {phi} = 1.66) were determined through the excitation/detection scheme of the Q{sub 2}(26.5) rotational line in the A{sup 2}{sigma}{sup +} - X{sup 2}{pi} (0,0) vibronic band and {gamma}(0,1) emission band. A calibration procedure by NO doping into the flame was applied to establish the NO concentration profiles in these flames. Chemiluminescent emission measurements in the (0, 0) vibronic emission bands of the OH{sup *} (A{sup 2}{sigma}{sup +} - X{sup 2}{pi}) and CH{sup *}(A{sup 2}{delta} - X{sup 2}{pi}) radicals were also obtained with high spatial and spectral resolution for fuel-rich premixed ethanol flames to correlate them with NO concentrations. Experimental chemiluminescence profiles and the ratios of the integrated areas under emission spectra (A{sub CH*}/A{sub CH*}(max.) and A{sub CH*}/A{sub OH*}) were determined. The relationships between chemiluminescence and NO concentrations were established along the premixed ethanol flames. There was a strong connection between CH{sup *} radical chemiluminescence and NO formation and the prompt-NO was identified as the governing mechanism for NO production. The results suggest the optimum ratio of the chemiluminescence of two radicals (A{sub CH*}/A{sub OH*}) for NO diagnostic purposes. (author)

  3. Infrared Chemiluminescence from XeF2-Silicon-Surface Reactions

    Microsoft Academic Search

    T. J. Chuang

    1979-01-01

    Infrared luminescence has been observed and the emission spectra in the 2.5-8.0-mum region determined for a silicon crystal exposed to XeF2 gas. In contrast to the broad and structureless emission in the uv and visible regions previously reported for some gassolid systems, the ir spectrum exhibits characteristic fine structure. Furthermore, the emitted radiation shows strong polarization and angular dependence indicating

  4. OH* Chemiluminescence: Pressure Dependence of O + H + M = OH* + M

    E-print Network

    Donato, Nicole

    2011-02-22

    . ...................................................... 24? Figure 10. A local sensitivity analysis with respect to OH* shows the most important reaction at the peak is R1: O + H + M = OH* + M at 1546K, 10.3 atm. For clarity, only the most influential reactions are shown...* concentration due to the change in rate of the given reaction. Figure 10 is a representative case where the local sensitivity analysis was performed at 1546 K, 10.3 atm. Positive values on the sensitivity plot lead to increased production of OH*, where...

  5. Determination of stimulants in a single human hair sample by high-performance liquid chromatographic method with chemiluminescence detection.

    PubMed

    Takayama, N; Tanaka, S; Hayakawa, K

    1997-01-01

    Stimulants that are controlled by the Stimulant Drug Control Law of Japan are methamphetamine (MA) and amphetamine (AP). MA is used by most stimulant addicts, and AP is detected as its main metabolite. We have developed a high-performance liquid chromatography method with chemiluminescence detection (CL-HPLC), for determining trace levels of MA and its metabolites in a single human hair sample, in which bis(2,4,6-trichlorophenyl)oxalate and hydrogen peroxide are the postcolumn reagents. After washing a single hair sample with water and methanol, it was cut into pieces, extracted with a mixed solution of methanol and hydrochloric acid for 1 h under ultra-sonication and allowed to stand at room temperature overnight. Then the organic phase was evaporated to dryness. To the residues, 0.1 mL of carbonate buffer and 0.1 mL of dansyl chloride solution were added and the solution was heated at 45 degrees C for 1 h. An aliquot of the reaction mixture was then subjected to HPLC. MA and AP were chemiluminogenically detected as their dansyl derivatives from a sample of only a single hair. The detection limit was about 2 pg in an injected volume (20 microliters), and about 20 pg in a single hair sample. This detection limit was smaller than that by the gas chromatography/mass spectrometry (selective ion monitoring) method. Our method was useful as a screening test for stimulant users. PMID:9051212

  6. Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV).

    PubMed

    Catalá-Icardo, Mónica; Lahuerta-Zamora, Luis; Torres-Cartas, Sagrario; Meseguer-Lloret, Susana

    2014-05-01

    A new, fast, selective and sensitive method has been developed for the simultaneous determination of nine organothiophosphorus (OTP) pesticides, namely omethoate, dimethoate, disulfoton-sulfoxide, methidathion, phosmet, malathion, diazinon, pirimiphos-methyl and chlorpyrifos. The pesticides were separated on a Kinetex C18 column by gradient elution with acetonitrile:water. A post-column basic hydrolysis of the pesticides and later a chemiluminescence (CL) reaction with cerium (IV) in acid medium was carried out. Hexadecylpyridinium chloride highly enhanced the CL emission. Under optimized conditions, linearity, precision, limits of detection and quantification, and accuracy were determined. Both selectivity and sensitivity were compared with those obtained with UV detection. In combination with SPE, limits of detection in the range 15-80ng/L and 5-30ng/L were obtained when 250mL and 1000mL of solution were treated, respectively. When applied to 250mL of sample the inter-day precision of the method was between 3.5% and 7.3% and the intra-day precision between 2.9% and 6.0%. The method was applied to determine OTP pesticides in spiked water samples from different origins: irrigation, river, sea, ground, spring, mineral and tap waters, being the percentage of recovery of added amounts near 100% form most of the pesticides. PMID:24685163

  7. Electrogenerated Chemiluminescence of BODIPY, Ru(bpy)32+, and 9,10-Diphenylanthracene Using Interdigitated Array Electrodes

    SciTech Connect

    Nepomnyashchii, Alexander B.; Kolesov, Grigory; Parkinson, Bruce A.

    2013-07-10

    Interdigitated array electrodes (IDAs) were used to produce steady-state electrogenerated chemiluminescence (ECL) by annihilation of oxidized and reduced forms of a substituted boron dipyrromethene (BODIPY) dye, 9,10-diphenylanthracene (DPA), and ruthenium(II) tris(bypiridine) (Ru-(bpy)32+). Digital simulations were in good agreement with the experimentally obtained currents and light outputs. Coreactant experiments, using tri-n-propylamine and benzoyl peroxide as a sacrificial homogeneous reductant or oxidant, show currents corresponding to electrode reactions of the dyes and not the oxidation or reduction of the coreactants. The results show that interdigitated arrays can produce stable ECL where the light intensity is magnified due to the larger currents as a consequence of feedback between generator and collector electrodes in the IDA. The light output for ECL is around 100 times higher than that obtained with regular planar electrodes with similar area. This material is based upon work supported as part of the Center of Molecular Electrocatalysis, an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences.

  8. Silver nanoparticles enhanced a novel TCPO-H2O2-safranin O chemiluminescence system for determination of 6-mercaptopurine.

    PubMed

    Biparva, Pourya; Abedirad, Seyed Mohammad; Kazemi, Sayed Yahya

    2015-06-15

    The present study deals with first attempt to introduce safranin O as the fluorophore for peroxyoxalate chemiluminescence system. The reaction of bis-(2,4,6-trichlorophenyl) oxalate (TCPO) with H2O2 catalyzed by silver nanoparticles can transfer energy to safranin O via the formation of dioxetanedione intermediate and emits orange-red light. The relationship between CL intensity and the concentration of TCPO, fluorophore, hydrogen peroxide and nanocatalyst was investigated. The Ag nanoparticles were synthesized by chemical reduction method and characterized using scanning electron microscopy, particle size analyzer and UV-spectroscopy. Moreover, the system was applied successfully to detect a drug, 6-mercaptopurine (6-MP) in pharmaceuticals. Under optimum conditions, a linear working range for 6-MP concentrations from 5.5×10(-7) to 5.5×10(-5)molL(-1) (r>0.9831, n=6) was obtained with a detection limit of 1.6×10(-7)molL(-1). The relative standard deviation for 6 repetitive determinations was less than 3.8% and recoveries of 98% and 103% were obtained. PMID:25796015

  9. Development of a wireless, self-sustaining damage detection sensor system based on chemiluminescence for structural health monitoring

    NASA Astrophysics Data System (ADS)

    Kuang, K. S. C.

    2014-03-01

    A novel application of chemiluminescence resulting from the chemical reaction in a glow-stick as sensors for structural health monitoring is demonstrated here. By detecting the presence of light emitting from these glow-sticks, it is possible to develop a low-cost sensing device with the potential to provide early warning of damage in a variety of engineering applications such as monitoring of cracks or damage in concrete shear walls, detecting of ground settlement, soil liquefaction, slope instability, liquefaction-related damage of underground structure and others. In addition, this paper demonstrates the ease of incorporating wireless capability to the sensor device and the possibility of making the sensor system self-sustaining by means of a renewable power source for the wireless module. A significant advantage of the system compared to previous work on the use of plastic optical fibre (POF) for damage detection is that here the system does not require an electrically-powered light source. Here, the sensing device, embedded in a cement host, is shown to be capable of detecting damage. A series of specimens with embedded glow-sticks have been investigated and an assessment of their damage detection capability will be reported. The specimens were loaded under flexure and the sensor responses were transmitted via a wireless connection.

  10. A 3D-printed device for a smartphone-based chemiluminescence biosensor for lactate in oral fluid and sweat.

    PubMed

    Roda, Aldo; Guardigli, Massimo; Calabria, Donato; Calabretta, Maria Maddalena; Cevenini, Luca; Michelini, Elisa

    2014-12-21

    Increasingly, smartphones are used as portable personal computers, revolutionizing communication styles and entire lifestyles. Using 3D-printing technology we have made a disposable minicartridge that can be easily prototyped to turn any kind of smartphone or tablet into a portable luminometer to detect chemiluminescence derived from enzyme-coupled reactions. As proof-of-principle, lactate oxidase was coupled with horseradish peroxidase for lactate determination in oral fluid and sweat. Lactate can be quantified in less than five minutes with detection limits of 0.5 mmol L(-1) (corresponding to 4.5 mg dL(-1)) and 0.1 mmol L(-1) (corresponding to 0.9 mg dL(-1)) in oral fluid and sweat, respectively. A smartphone-based device shows adequate analytical performance to offer a cost-effective alternative for non-invasive lactate measurement. It could be used to evaluate lactate variation in relation to the anaerobic threshold in endurance sport and for monitoring lactic acidosis in critical-care patients. PMID:25343380

  11. Free Radicals and Chemiluminescence as Products of the Spontaneous Oxidation of Sulfide in Seawater, and Their Biological Implications

    PubMed Central

    TAPLEY, DAVID W.; BUETTNER, GARRY R.; SHICK, J. MALCOLM

    2014-01-01

    The discovery of symbioses between marine invertebrates and sulfide-oxidizing bacteria at deep-sea hydrothermal vents and in other high-sulfide marine environments has stimulated research into the adaptations of metazoans to potentially toxic concentrations of sulfide. Most of these studies have focused on a particular action of sulfide—its disruption of aerobic metabolism by the inhibition of mitochondrial respiration—and on the adaptations of sulfide-tolerant animals to avoid this toxic effect (1). We propose that sulfidic environments impose another, hitherto overlooked type of toxicity: exposure to free radicals of oxygen, which may be produced during the spontaneous oxidation of sulfide, thus imposing an oxidative stress. Here we present evidence that oxygen- and sulfur-centered free radicals are produced during the oxidation of sulfide in seawater, and we propose a reaction pathway for sulfide oxidation that is consistent with our observations. We also show that chemiluminescence at visible wavelengths occurs during sulfide oxidation, providing a possible mechanism for the unexplained light emission from hydrothermal vents (2, 3). PMID:25575385

  12. Characterization and mitigation of water vapor effects in the measurement of ozone by chemiluminescence with nitric oxide

    NASA Astrophysics Data System (ADS)

    Boylan, P.; Helmig, D.; Park, J.-H.

    2014-05-01

    Laboratory experiments were conducted to investigate the effects of water vapor on the reaction of nitric oxide with ozone in a gas-phase chemiluminescence instrument used for fast response and high sensitivity detection of atmospheric ozone. Water vapor was introduced into a constant level ozone standard and both ozone and water vapor signals were recorded at 10 Hz. The presence of water vapor was found to reduce, i.e. quench, the ozone signal. A dimensionless correction factor was determined to be 4.15 ± 0.14 × 10-3, which corresponds to a 4.15% increase in the corrected ozone signal per 10 mmol mol-1 of co-sampled water vapor. An ozone-inert water vapor permeable membrane (a Nafion dryer with a counterflow of dry air from a compressed gas cylinder) was installed in the sampling line and was shown to remove the bulk of the water vapor in the sample air. At water vapor mole fractions above 25 mmol mol-1, the Nafion dryer removed over 75% of the water vapor in the sample. This reduced the required ozone signal correction from over 11% to less than 2.5%. The Nafion dryer was highly effective at reducing the fast fluctuations of the water vapor signal (more than 97%) while leaving the ozone signal unaffected, which is a crucial improvement for minimizing the quenching interference of water vapor fluxes and required density correction in the determination of ozone fluxes by the eddy covariance technique.

  13. A chemiluminescent-western blot assay for quantitative detection of Plasmodium falciparum circumsporozoite protein.

    PubMed

    Kumar, Sanjai; Zheng, Hong; Sangweme, Davison T; Mahajan, Babita; Kozakai, Yukiko; Pham, Phuong T; Morin, Merribeth J; Locke, Emily; Kumar, Nirbhay

    2013-04-30

    Highly sensitive and reliable assays based on the quantitation of immunologically relevant component(s) in recombinant or whole parasite-based vaccines would facilitate pre-clinical and clinical phases and the monitoring of malaria vaccine deployment. Here we report a laboratory-grade Western Blot assay for quantitative detection of Plasmodium falciparum circumsporozoite protein (PfCSP) in P. falciparum sporozoite (PfSPZ) and in recombinant (rPfCSP) product. This assay is based on the immuno-reactivity of an anti-P. falciparum CSP monoclonal antibody (mAb 2A10) with the NANP-repeat units on PfCSP. The antigen-antibody complex is detected by reaction with a commercially obtained chemiluminescence-linked Immunodetection system. The linear range for detecting the recombinant P. falciparum CSP (rPfCSP) in this assay is 3-12pg (R(2)=0.9399). The range for detecting the day 15 salivary-gland PfSPZ is between 0.0625 and 1 parasite (R(2)=0.9448) and approximately 10.0pg of PfCSP was detected on each sporozoite. The assay was highly reproducible in measuring the PfCSP on PfSPZ. The inter-assay Coefficient of Variation (CV%) was 10.31% while the intra-assay CV% on three different days was 6.05%, 2.03% and 1.42% respectively. These results suggest that this ECL-WB assay is highly sensitive and robust with a low degree of inter-assay and intra-assay variations. To our knowledge, this is the most sensitive immunoassay for the detection of a recombinant or native malarial protein and may have a wider range of applications including the quantification of immunological component(s) in a vaccine formulation, determination of the antigenic integrity in adjuvanted-vaccine and in stability studies. In addition, this assay can be applied to measure the mosquito infectivity in malaria transmission areas and to determine the effects of intervention measures on malaria transmission. PMID:23399449

  14. On use of CO{sub 2} chemiluminescence for combustion metrics in natural gas fired reciprocating engines.

    SciTech Connect

    Gupta, S. B.; Bihari, B.; Biruduganti, M.; Sekar, R.; Zigan, J. (Energy Systems); (Cummins Technical Center)

    2011-01-01

    Flame chemiluminescence is widely acknowledged to be an indicator of heat release rate in premixed turbulent flames that are representative of gas turbine combustion. Though heat release rate is an important metric for evaluating combustion strategies in reciprocating engine systems, its correlation with flame chemiluminescence is not well studied. To address this gap an experimental study was carried out in a single-cylinder natural gas fired reciprocating engine that could simulate turbocharged conditions with exhaust gas recirculation. Crank angle resolved spectra (266-795 nm) of flame luminosity were measured for various operational conditions by varying the ignition timing for MBT conditions and by holding the speed at 1800 rpm and Brake Mean effective Pressure (BMEP) at 12 bar. The effect of dilution on CO*{sub 2}chemiluminescence intensities was studied, by varying the global equivalence ratio (0.6-1.0) and by varying the exhaust gas recirculation rate. It was attempted to relate the measured chemiluminescence intensities to thermodynamic metrics of importance to engine research -- in-cylinder bulk gas temperature and heat release rate (HRR) calculated from measured cylinder pressure signals. The peak of the measured CO*{sub 2} chemiluminescence intensities coincided with peak pressures within {+-}2 CAD for all test conditions. For each combustion cycle, the peaks of heat release rate, spectral intensity and temperature occurred in that sequence, well separated temporally. The peak heat release rates preceded the peak chemiluminescent emissions by 3.8-9.5 CAD, whereas the peak temperatures trailed by 5.8-15.6 CAD. Such a temporal separation precludes correlations on a crank-angle resolved basis. However, the peak cycle heat release rates and to a lesser extent the peak cycle temperatures correlated well with the chemiluminescent emission from CO*{sub 2}. Such observations point towards the potential use of flame chemiluminescence to monitor peak bulk gas temperatures as well as peak heat release rates in natural gas fired reciprocating engines.

  15. Molecular Physics, Vol. 105, Nos. 57, 10 March10 April 2007, 715725 The high resolution Fourier-transform chemiluminescence spectrum

    E-print Network

    Ashworth, Stephen H.

    -transform chemiluminescence spectrum of the HS2 radical S. H. ASHWORTH*y and E. H. FINKz yUniversity of East Anglia, Norwich November 2006) The chemiluminescence spectrum of the HS2 radical has been recorded with a Fourier-transform spectrometer. The overview spectrum in the region 9000 cmÀ1 to 4000 cmÀ1 has been analysed and the vibrational

  16. beta 2-glycoprotein I (apolipoprotein H) modulates uptake and endocytosis associated chemiluminescence in rat Kupffer cells.

    PubMed

    Gomes, L F; Gonçalves, L M; Fonseca, F L A; Celli, C M; Videla, L A; Chaimovich, H; Junqueira, V B C

    2002-07-01

    beta 2-Glycoprotein I (beta 2 GPI) is known to influence macrophage uptake of particles with phosphatidylserine containing surfaces, as apoptotic thymocytes and unilamellar vesicles in vitro. Nevertheless, effects upon macrophage activation induced by this interaction are still unknown. beta 2 GPI influence upon the reactive species production by Kupffer cells was evaluated in order to investigate whether beta 2 GPI modulates the macrophage response to negatively charged surfaces. Chemiluminescence of isolated non-parenchymal rat liver cells was measured after phagocytosis of opsonized zymosan or phorbolymristate acetate (PMA) stimulation, in the presence and absence of large unilamellar vesicles (LUVs) containing 25 mol% phosphatidylserine (PS) or 50 mol% cardiolipin (CL) and complementary molar ratio of phosphatidylcholine (PC). beta 2 GPI decreased by 50% the chemiluminescence response induced by opsonized zymosan, with a 66% reduction of the initial light emission rate. PMA stimulated Kupffer cell chemiluminescence was insensitive to human or rat beta 2 GPI. Albumin (500 micrograms/ml) showed no effect upon chemiluminescence. beta 2 GPI increased PS/PC LUV uptake and degradation by Kupffer cells in a concentration-dependent manner, without leakage of the internal contents of the LUVs, as shown by fluorescence intensity enhancement. LUVs opsonized with antiphospholipid antibodies (aPL) from syphilitic patients increased light emission by Kupffer cells. Addition of beta 2 GPI to the assay reduced chemiluminescence due to opsonization with purified IgG antibodies from systemic lupus erythematosus (SLE or syphilis (Sy) patient sera. A marked net increase in chemiluminescence is observed in the presence of Sy aPL antibodies, whereas a decrease was found when SLE aPL were added to the assay, in the presence or absence of beta 2 GPI. At a concentration of 125 micrograms/ml, beta 2 GPI significantly reduced Kupffer cell Candida albicans phagocytosis index and killing score by 50 and 10%, respectively. The present data strongly suggest that particle uptake in the presence of beta 2 GPI is coupled to an inhibition of reactive species production by liver macrophages during the respiratory burst, supporting the role of beta 2 GPI as a mediator of senescent cell removal. PMID:12180124

  17. Stimulation of Ca2+-dependent chemiluminescence in rat polymorphonuclear leucocytes by polystyrene beads and the non-lytic action of complement.

    PubMed Central

    Hallett, M B; Luzio, J P; Campbell, A K

    1981-01-01

    (1) Chemiluminenscence of rat polymorphonuclear leukocytes was stimulated by a phagocytic stimulus, latex beads (diameter = 1.01 micrometer). The maximum chemiluminescent intensity increased with bead concentration in the range 0.2--20 x 10(9) beads/ml. This response was abolished in the absence of extracellular Ca2+ (1 mM EGTA). (2) Chemiluminescence could also be stimulated by the Ca2+ ionophore A23187 in the presence of extra-cellular calcium. (3) Addition of human serum, as a source of complement, to rat polymorphonuclear leukocytes preincubated with anti-5'-nucleotidase serum resulted in a rapid stimulation of chemiluminescence, after a lag of about 40 s. (4) The stimulation of chemiluminescence by antibody plus complement was not the result of cell lysis because (i) no significant release of lactate dehydrogenase was detected at the time of the chemiluminescent response (ii) chemiluminescence was associated with the cells and not the surrounding media (iii) cell lysis did not produce chemiluminescence. (5) Chemiluminescence stimulated by antibody plus complement or by beads was inhibited by the 'calmodulin-blocker', trifluoperazine (50% inhibiton with approximately 20--30 microM). (6) Cu2+ (10(-4) M), which can inhibit C9 action, inhibited the rapid rise in chemiluminescence induced by antibody plus complement, but not the bead-induced chemiluminescence. (7) Depletion of C9 from human serum markedly inhibited the complement induced chemiluminescence response. Addition of purified C9 restored the response. (8) It was concluded that formation of the terminal complement attack complex at the surface of rat polymorphonuclear leucocytes induces a Ca2+-dependent chemiluminescence in the cells, in the absence of cell lysis. PMID:7319554

  18. Effect of glutathione on peroxyoxalate chemiluminescence of hypericin as the fluorophore

    NASA Astrophysics Data System (ADS)

    Kazemi, Sayed Yahya; Abedirad, Seyed Mohammad

    2014-01-01

    Herein, the effect of amino acid Glutathione (GSH) on Peroxyoxalate Chemiluminescence was studied for the first time. Hypericin (HYP) was employed as the efficient fluorophore. The investigated parameters included rise and fall rate constant for the chemiluminescence burst, theoretical and experimental maximum intensity, the time-needed to reach maximum intensity and the total light yield emission which theoretically was evaluated using the pooled intermediate model by a computerized non-linear least-squares curve fitting program (KINFIT). Furthermore, based on observed quenching effect of GSH, the Stern-Volmer plot in quencher concentration range of 2.8 × 10-6 to 3.4 × 10-5 M with KQ value of 1.59 × 104 was calculated. The bimolecular quenching rate constant (Kq) was also estimated about 2.8 × 1012 and M-1 S-1. Moreover the system was applied successfully to determine glutathione in biological samples.

  19. Effect of glutathione on peroxyoxalate chemiluminescence of hypericin as the fluorophore.

    PubMed

    Kazemi, Sayed Yahya; Abedirad, Seyed Mohammad

    2014-01-24

    Herein, the effect of amino acid Glutathione (GSH) on Peroxyoxalate Chemiluminescence was studied for the first time. Hypericin (HYP) was employed as the efficient fluorophore. The investigated parameters included rise and fall rate constant for the chemiluminescence burst, theoretical and experimental maximum intensity, the time-needed to reach maximum intensity and the total light yield emission which theoretically was evaluated using the pooled intermediate model by a computerized non-linear least-squares curve fitting program (KINFIT). Furthermore, based on observed quenching effect of GSH, the Stern-Volmer plot in quencher concentration range of 2.8×10(-6) to 3.4×10(-5)M with KQ value of 1.59×10(4) was calculated. The bimolecular quenching rate constant (Kq) was also estimated about 2.8×10(12) and M(-1) S(-1). Moreover the system was applied successfully to determine glutathione in biological samples. PMID:24152863

  20. Chemiluminescence detection of glucose using Ce(IV) oxidation in a batch system.

    PubMed

    Nagai, Megumi; Miyahara, Wakayo; Sagawa, Honami; Yamazaki, Shigeo; Saito, Keiitsu

    2013-01-01

    A chemiluminescence method for the determination of glucose (GC) using a batch system made in our laboratory is proposed. The method is based on measures of the chemiluminesence intensity from a batch of sample GC solutions following the injection of an (NH(4))(2)[Ce(NO(3))(6)]-[Ru(bpy)(3)]Cl(2) solution. The effect of the Ru complex concentration on chemiluminescence was studied. The detection limit was 0.5 × 10(-8) M, and the linear calibration curve reached 10(-7) M. The Ru complex was selected on the basis of its desirable performance compared to those of other photosensitizers. However, flow-injection analysis (FIA) using the Ce-Ru complex reagent was not applicable for the detection of GC. PMID:23303079

  1. Determination of hydrogen peroxide by flow injection analysis with aryl oxalate-sulforhodamine 101 chemiluminescence

    SciTech Connect

    Katayama, M.; Takeuchi, H.; Tanigchi, H. (Meiji Coll. of Pharmacy, Tokyo (Japan))

    1991-06-01

    A flow injection analysis (FIA) method for the determination of hydrogen peroxide by aryl oxalate chemiluminescence detection was studied. The analyte was detected by using sulforhodamine 101 as a new fluorophore and bis (4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl)oxalate (TDPO) in imidazole buffer (pH 7.0)-acetonitrile. The detection limit was 3.0 {times} 10{sup {minus}9}M. The relative standard deviation (n=6) for 1.0 {times} 10{sup {minus}6} M hydrogen peroxide was 2.8%. This FIA method was shown to be 20 to 2,500 times more sensitive than the previous FIA method with aryl oxalate chemiluminescence.

  2. Molecular detection of bovine viral diarrhea virus in clinical samples by polymerase chain reaction-chemiluminscent DNA hybridization assay

    Microsoft Academic Search

    G. S. Radwan

    2002-01-01

    The polymerase chain reaction (PCR) followed by chemiluminescent probe hybridization assay was used for the detection of bovine viral diarrhea virus (BVDV) infections in cattle. BVDV RNA's from individual clinical specimens, from persistently infected (PI) and acutely diseased cattle were transcribed to cDNA using reverse transcriptase. Using a set of 18-mer oligonucleotide primers located within the conserved 5' untranslated region

  3. Coupling microdialysis with flow-injection chemiluminescence detection for a protein–drug interaction study

    Microsoft Academic Search

    Hua Chen; Zhengjun Gong; Zhujun Zhang

    2006-01-01

    The interaction of metronidazole (MTZ) and human serum albumin (HSA) was studied using the coupling system of on-line microdialysis sampling with flow-injection chemiluminescence detection (FI-MD-CL). The interested drug and HSA were mixed in different molar ratios in 0.067molL?1 phosphate buffer (pH 7.4) and incubated at 37°C in a water-bath. Then the microdialysis probe was put into the MTZ–HSA mixed solution

  4. Liquid chromatographic determination of cyclosporin A using aryl oxalate chemiluminescence detection

    Microsoft Academic Search

    Masatoki Katayama; Hirokazu Taniguchi; Yoshifumi Matsuda; Sumiyuki Akihama; Izumi Hara; Hirohisa Sato; Satoru Kaneko; Yukako Kuroda; Shiro Nozawa

    1995-01-01

    A new liquid chromatographic method based on aryl oxalate chemiluminescence detection for the determination of immunosuppressing drugs like cyclosporin A is described. Cyclosporin A was separated using a Zorbax C8 analytical column with 0.1 M hydrogen peroxide in methanol-water (95 + 5) as eluent, and could be determined from 5.0 × 10?9 to 2.0 × 10?2 M (signal-to-noise ratio =

  5. Near-Ir Electrogenerated Chemiluminescence of Tricarbocyanine Dyes in Micellar Systems

    Microsoft Academic Search

    Sang Kwon Lee; Allen J. Bard

    1998-01-01

    Two tricarbocyanine near-IR dyes containing the same (dimethyl naphthalindole) heterocyclic nuclei, IR144 and IR125, were studied in organic solvents, in water, and in the presence of certain surfactants. The anodic oxidation of IR144 produces electrogenerated chemiluminescence (ECL) in the presence of tri-n-propylamine as a coreactant in sodium dodecyl sulfate surfactant solution or in MeCN\\/DMSO (2:1 v\\/v) solution. The one-electron oxidation

  6. Quantitative determination of aqueous-phase ozone by chemiluminescence using indigo5,5'-disulfonate

    Microsoft Academic Search

    Koji. Takeuchi; Takashi. Ibusuki

    1989-01-01

    Indigo-5,5'-disulfonate (IDS) was found to be an efficient reagent for the determination of ozone by chemiluminescence (CL); hence it was applied to the continuous measurements of dissolved ozone (Oâ(aq)). The optimum reagent composition was determined as 10 mg L-1 IDS plus 2 mM phosphate (pH 7.2). The CL intensity was proportional to the Oâ(aq) concentration in the range of 0.025-410

  7. Determination of Glyphosate as N-Nitroso Derivative by High Performance Liquid Chromatography with Chemiluminescence Detection

    Microsoft Academic Search

    N. P. Sen; P. A. Baddoo

    1996-01-01

    Glyphosate (GP) is a non-selective, postemergence herbicide widely used for weed and vegetative control. This paper describes a method for the determination of GP that is based on: a) nitrosation of GP to N-nitroso-GP (NGP), b) HPLC separation on an anion-exchange column, c) post column denitrosation of NGP to nitric oxide, and d) chemiluminescence determination of the liberated nitric oxide

  8. Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin M1 in milk.

    PubMed

    Vdovenko, Marina M; Lu, Chuan-Chen; Yu, Feng-Yih; Sakharov, Ivan Yu

    2014-09-01

    A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for detecting aflatoxin M1 (AFM1) was developed. To improve the sensitivity of the assay, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used to enhance peroxidase-induced CL. The concentrations of the coating anti-AFM1 antibody and the conjugate of AFB1 with horseradish peroxidase the conditions of the chemiluminescent assay were varied to optimise the condition of the chemiluminescent assay. The lower detection limit values and dynamic working range of CL-ELISA of AFM1 were 0.001 ng mL(-1) and 0.002-0.0075 ng mL(-1), respectively. A 20-fold dilution of milk samples prevented a matrix effect of the milk and allowed measurement of AFM1 at concentrations below than the maximum acceptable limit. Values of recovery within and between assays were 81.5-117.6% and 86-110.6%, respectively. The results of using the developed CL-ELISA to analyse samples of six brands of milk that were purchased in Taiwan revealed that AFM1 was absent from all studied samples. PMID:24731347

  9. Development of ultrasensitive direct chemiluminescent enzyme immunoassay for determination of aflatoxin B1 in food products.

    PubMed

    Yu, Feng-Yih; Gribas, Anastasia V; Vdovenko, Marina M; Sakharov, Ivan Yu

    2013-03-30

    A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for determination of aflatoxin B1 (AFB1) was developed. To improve the assay sensitivity, a mixture of 3-(10'-phenothiazinyl)-propane-1-sulfonate and 4-morpholinopyridine previously optimized by a factorial design was used as enhancer of horseradish peroxidase-induced chemiluminescence. Varying the concentrations of the coating anti-AFB1 antibody and conjugate of AFB1 and horseradish peroxidase the conditions of the chemiluminescent assay were optimized. The values of the detection limit value and dynamic working range of CL-ELISA of AFB1 were 0.0015 ng mL(-1) and 0.003-0.03 ng mL(-1), respectively. It was shown that a dilution of rice and mung beans extracts in 5 and 10 times, respectively, prevented a matrix effect of the food products in CL-ELISA. The recovery values from the spiked samples of rice and mung beans were in the range of 90-104% and 102-117%, respectively. Studying 8 rice and 8 mung beans samples purchased in commercial stores the developed CL-ELISA allowed to find 3 samples (1 rice and 2 mung beans) containing AFB1, the content of AFB1 in one sample being higher than the maximum acceptable level established in the European Community. PMID:23598187

  10. Analysis of chemiluminescence measurements by grey-scale ICCD and colour digital cameras

    NASA Astrophysics Data System (ADS)

    Migliorini, F.; Maffi, S.; De Iuliis, S.; Zizak, G.

    2014-05-01

    Spectral, grey-scale and colour chemiluminescence measurements of C2* and CH* radicals' emission are carried out on the flame front of a methane-air premixed flame at different equivalence ratios. To this purpose, properly spatially resolved optical equipment has been implemented in order to reduce the background emission from other burned gas regions. The grey-scale (ICCD + interference filters) and RGB colour (commercial digital camera) approaches have been compared in order to find a correspondence between the C2* and the green component, as well as the CH* and the blue component of the emission intensities. The C2*/CH* chemiluminescence ratio has been investigated at different equivalence ratios and a good correlation has been obtained, showing the possibility of sensing the equivalence ratio in practical systems. The grey-scale and colour chemiluminescence analysis has then been applied to a meso-scale not premixed swirl combustor fuelled with a methane-air mixture and operating at 0.3 MPa. 2D results are presented and discussed in this work.

  11. Chemiluminescence-based pesticide biosensor utilizing the intelligent evolved properties of the enzyme alkaline phosphatase

    SciTech Connect

    Ayyagari, M.; Kamtekar, S.; Pande, R.; Marx, K.; Kumar, J.

    1994-12-31

    A methodology is described for immobilizing the enzyme alkaline phosphatase onto a glass surface using a novel biotinylated copolymer, poly(3-undecylthiophene-co-3- methanoithiophene). A streptavidin conjugate of alkaline phosphatase is used in this study. The biotinylated polymer is attached to the silanized glass surface via hydrophobic interactions and the enzyme is interfaced with the polymer through the classical biotin- streptavidin interaction. Alkaline phosphatase catalyzes the dephosphorylation of a macrocyclic compound, chloro-3-(4-methoxy spiro) (1,2 dioxetane-3-2`-tricyclo-) (3.3.1.1 )-(decani-4-yl) phenyl phosphate, to a species which emits energy by chemiluminescence. This chemiluminescence signal can be detected with a photomultiplier tube for enzymatic catalysis with the biocatalyst both in solution and immobilized on a glass surface. The signal generation is inhibited by the organophosphorus based insecticides such as paraoxon as well as nerve agents. We demonstrate in this study that a number of organophosphorus based insecticides inhibit the enzyme-mediated generation of chemiluminescence signal. This is true for the enzyme conjugate both free in solution and immobilized on a glass surface. In solution, the inhibition resembles the case of a partially uncompetitive system. By this type of inhibition we are able to detect pesticides down to about 50 ppb for the enzyme in solution. The pesticide detection limit of immobilized enzyme is currently being investigated. The enzyme is capable of a number of measurement cycles without significant loss of signal level.

  12. Chemiluminescent examination of abiotic oxidative stress of watercress.

    PubMed

    Beals, Christopher; Byl, Thomas

    2014-04-01

    Watercress (Nasturtium officinale) is an aquatic plant that readily bioaccumulates heavy metals that may be found in contaminated aquatic systems. Toxic effects of contaminants on the physiological processes cause changes in oxidase enzymatic activity in watercress, which can be measured with a luminometer. The luminometer uses the reaction produced when peroxidases break down hydrogen peroxide into water and an oxygen radical. The resulting oxyradical binds to and oxidizes phenolic groups, producing a measureable luminescent reaction. Nasturtium officinale plants were exposed to 3 different concentrations of heavy metals, including lead, nickel, copper, and manganese for 24 h, 48 h, and 72 h. Aquatic exposure to the 4 heavy metals caused a significant increase in oxidative enzyme production. Fluorometric and morphometric measurements were also conducted to compare plant stress with the oxidative enzyme analyses. Fluorometric measurements performed on plants stressed by exposure to heavy metals revealed no significant decreases in photosystem II efficiency. Morphometric measurements of root length showed decreased root growth resulting from exposures to Ni, Cu, and Mn. PMID:24306856

  13. Slope effects on the fluid dynamics of a fire spreading across a fuel bed: PIV measurements and OH* chemiluminescence imaging

    NASA Astrophysics Data System (ADS)

    Morandini, F.; Silvani, X.; Honoré, D.; Boutin, G.; Susset, A.; Vernet, R.

    2014-08-01

    Slope is among the most influencing factor affecting the spread of wildfires. A contribution to the understanding of the fluid dynamics of a fire spreading in these terrain conditions is provided in the present paper. Coupled optical diagnostics are used to study the slope effects on the flow induced by a fire at laboratory scale. Optical diagnostics consist of particle image velocimetry, for investigating the 2D (vertical) velocity field of the reacting flow and chemiluminescence imaging, for visualizing the region of spontaneous emission of OH radical occurring during gaseous combustion processes. The coupling of these two techniques allows locating accurately the contour of the reaction zone within the computed velocity field. The series of experiments are performed across a bed of vegetative fuel, under both no-slope and 30° upslope conditions. The increase in the rate of fire spread with increasing slope is attributed to a significant change in fluid dynamics surrounding the flame. For horizontal fire spread, flame fronts exhibit quasi-vertical plume resulting in the buoyancy forces generated by the fire. These buoyancy effects induce an influx of ambient fresh air which is entrained laterally into the fire, equitably from both sides. For upward flame spread, the induced flow is strongly influenced by air entrainment on the burnt side of the fire and fire plume is tilted toward unburned vegetation. A particular attention is paid to the induced air flow ahead of the spreading flame. With increasing the slope angle beyond a threshold, highly dangerous conditions arise because this configuration induces wind blows away from the fire rather than toward it, suggesting the presence of convective heat transfers ahead of the fire front.

  14. Chemiluminescent evaluation of peroxide value in olive oil.

    PubMed

    Stepanyan, Vahan; Arnous, Anis; Petrakis, Christos; Kefalas, Panagiotis; Calokerinos, Antony

    2005-02-28

    A method is described for the evaluation of the peroxide value (PV, meq. O(2) kg(-1)) in olive oil. The method is based on the chemiluminogenic energy-transfer reaction of bis(2,4,6-(trichlorophenyl)oxalate (TCPO) with hydrogen peroxide or total peroxides in the presence of Mn(II) as catalyst and 9,10-dimethylanthracene as fluorophore. The procedure developed allows the evaluation of PV within the range of 0.6-100meq. O(2) kg(-1) (CL intensity = 1.76 x PV (meq. O(2) kg(-1)) + 23.2, r(2) = 0.994, n = 9) and relative standard deviation within the range 1-5% by using a simple manual measurement. PMID:18969910

  15. FI-chemiluminometric study of thiazides by on-line photochemical reaction.

    PubMed

    Ciborowski, M; Icardo, M Catalá; Mateo, J V García; Martínez Calatayud, J

    2004-11-19

    The present manuscript deals with a simple and sensitive flow-injection method for the chemiluminescent determination of thiazides. The method is based on the on-line photodegradation and chemiluminescent determination of the resulting photo-fragments. The on-line photodegradation is performed in basic medium by using a photoreactor consisting of a 550cm long x 0.8mm ID piece of PTFE tubing helically coiled around an 8W low-pressure mercury lamp. The determination of the photo-irradiated thiazides is performed by a chemiluminescent oxidative reaction with Ce(IV) in sulphuric acid medium. A heterogeneous group of thiazides (indapamide, metolazone, hydroflumethiazide, chlorthalidone and bendroflumethiazide) has been studied. Hydrochlorothiazide was selected as a test substance. The "on-line" photochemical reaction approach allows the sensitive chemiluminescent determination of thiazides which do not present native chemiluminescence in the absence of sensitizers such as Rhodamine 6G. Linear calibration graphs were typically over the range 0.5-12 microgml(-1) (indapamide, metolazone, hydroflumethiazide and chlorthalidone); and over the range 0.5-5 microgml(-1) (hydrochlorothiazide). Limits of detection ranged between 0.005 microgml(-1) (hydrochlorothiazide) and 0.06 microgml(-1) (bendroflumethiazide). The relative standard deviation for the test substance was 2.0% for 2 microgl(-1) of the drug (n = 11) and the throughput was 65 h(-1) in all cases. The assessment of the photodegradation step on the molecular structure of thiazides was established by recording UV and fluorimetric spectra. The viability of the on-line photoinduced fluorescent determination of hydroflumethiazide and bendroflumethiazide was confirmed. The method was also applied to the determination of hydrochlorothiazide in commercially available formulation. PMID:15533660

  16. Attenuation of tumor necrosis factor-induced endothelial cell cytotoxicity and neutrophil chemiluminescence

    SciTech Connect

    Zheng, H.; Crowley, J.J.; Chan, J.C.; Hoffmann, H.; Hatherill, J.R.; Ishizaka, A.; Raffin, T.A. (Stanford Univ. Medical Center, CA (USA))

    1990-11-01

    Our laboratory has previously shown that the administration of tumor necrosis factor (TNF), a cytokine produced by activated mononuclear cells, to guinea pigs produces a syndrome similar to gram-negative sepsis or ARDS. Pentoxifylline (PTX), a methylxanthine, protects against TNF-induced and sepsis-induced acute lung injury in vivo. We now report on in vitro cellular studies of PMN-mediated cellular injury and its attenuation. We studied TNF-induced bovine pulmonary artery endothelial cell (EC) cytotoxicity both with and without PMN. A 51Cr release assay was used to measure EC damage. Further, we investigated PMN function in response to TNF by measuring chemiluminescence. Agents that attenuate EC damage and PMN activation were evaluated in the above assays. Results revealed that TNF causes EC injury (p less than 0.05) and PMN increase TNF-induced EC injury. Furthermore, PTX, aminophylline (AMPH), caffeine, and forskolin attenuate TNF-induced EC cytotoxicity only in the presence of PMN (p less than 0.05). Of interest, dibutyryl cAMP (DBcAMP) protects EC from TNF-induced injury both with and without PMN. Agents that may increase cAMP levels in PMN (PTX, DBcAMP, forskolin, isobutyl methylxanthine, and terbutaline) significantly attenuate TNF-induced PMN chemiluminescence (p less than 0.05). We conclude that TNF causes EC damage and PMN increase this damage. Furthermore, PTX, AMPH, caffeine, and forskolin can attenuate TNF-induced EC injury in the presence of PMN, whereas DBcAMP attenuates TNF-induced EC injury with and without PMN. In addition, agents that may increase intracellular cAMP levels in PMN can attenuate TNF-induced PMN chemiluminescence. Thus, these agents likely attenuate TNF-induced PMN-mediated EC injury through their inhibitory effects on PMN.

  17. Toward complete miniaturisation of flow injection analysis systems: microfluidic enhancement of chemiluminescent detection.

    PubMed

    Gracioso Martins, Ana M; Glass, Nick R; Harrison, Sally; Rezk, Amgad R; Porter, Nichola A; Carpenter, Peter D; Du Plessis, Johan; Friend, James R; Yeo, Leslie Y

    2014-11-01

    Conventional flow injection systems for aquatic environmental analysis typically comprise large laboratory benchscale equipment, which place considerable constraints for portable field use. Here, we demonstrate the use of an integrated acoustically driven microfluidic mixing scheme to enhance detection of a chemiluminescent species tris(2,2'-bipyridyl)dichlororuthenium(II) hexahydrate-a common chemiluminescent reagent widely used for the analysis of a wide range of compounds such as illicit drugs, pharmaceuticals, and pesticides-such that rapid in-line quantification can be carried out with sufficient on-chip sensitivity. Specifically, we employ surface acoustic waves (SAWs) to drive intense chaotic streaming within a 100 ?L chamber cast in polydimethoxylsiloxane (PDMS) atop a microfluidic chip consisting of a single crystal piezoelectric material. By optimizing the power, duration, and orientation of the SAW input, we show that the mixing intensity of the sample and reagent fed into the chamber can be increased by one to two orders of magnitude, leading to a similar enhancement in the detection sensitivity of the chemiluminescent species and thus achieving a theoretical limit of detection of 0.02 ppb (0.2 nM) of l-proline-a decade improvement over the industry gold-standard and two orders of magnitude more sensitive than that achievable with conventional systems-simply using a portable photodetector and without requiring sample preconcentration. This on-chip microfluidic mixing strategy, together with the integrated miniature photodetector and the possibility for chip-scale microfluidic actuation, then alludes to the attractive possibility of a completely miniaturized platform for portable field-use microanalytical systems. PMID:25275830

  18. Multiplexed chemiluminescent assays in ArrayPlates for high-throughput measurement of gene expression

    NASA Astrophysics Data System (ADS)

    Martel, Ralph R.; Rounseville, Matthew P.; Botros, Ihab W.; Seligmann, Bruce E.

    2002-06-01

    Multiplexed Molecular Profiling (MMP) assays for drug discovery are performed in ArrayPlates. ArrayPlates are 96- well microtiter plates that contain a 16-element array at the bottom of each well. Each element within an array measures one analyte in a sample. A CCD imager records the quantitative chemiluminescent readout of all 1,536 elements in a 96-well plate simultaneously. Since array elements are reagent modifiable by the end-user, ArrayPlates can be adapted to a broad range of nucleic acid- and protein-based assays. Such multiplexed assays are rapidly established, flexible, robust, automation-friendly and cost-effective. Nucleic acid assays in ArrayPlates can detect DNA and RNA, including SNPs and ESTs. A multiplexed mRNA assay to measure the expression of 16 genes is described. The assay combines a homogeneous nuclease protection assay with subsequent probe immobilization to the array by means of a sandwich hybridization followed with chemiluminescent detection. This assay was used to examine cells grown and treated in microplates and avoided cloning, transfection, RNA insolation, reverse transcription, amplification and fluorochrome labeling. Standard deviations for the measurement of 16 genes ranged from 3 percent to 13 percent in samples of 30,000 cells. Such ArrayPlates transcription assays are useful in drug discovery and development for target validation, screening, lead optimization, metabolism and toxicity profiling. Chemiluminescent detection provides ArrayPlates assays with high signal-to-noise readout and simplifies imager requirements. Imaging a 2D surface that contains arrays simplifies lens requirements relative to imaging columns of liquid in microtiter plate wells. The Omix imager for ArrayPlates is described.

  19. Experiments concerning the laser-enhanced reaction between vibrationally excited O3 and NO

    NASA Technical Reports Server (NTRS)

    Hui, K.-K.; Cool, T. A.

    1978-01-01

    The enhancement in reaction rate between O3 and NO is studied for the case of O3 vibrationally excited by a CO2 laser. Chemiluminescence observations of a vibrationally excited and an electronically excited nitrogen dioxide reaction product provide information on the separate contributions to the overall reaction rate of these two reactive channels. The contribution of the stretching and bending modes of O3 to the reaction rate enhancement is also discussed. In addition, consideration is given to the nonreactive vibrational deactivation of vibrationally excited O3.

  20. Application of magnetic nanoparticles in full-automated chemiluminescent enzyme immunoassay

    NASA Astrophysics Data System (ADS)

    Xie, Xiaomao; Ohnishi, Noriyuki; Takahashi, Yuki; Kondo, Akihiko

    2009-05-01

    The magnetic nanoparticles (MNPs) Therma-Max™ were used as a carrier to develop an automated sandwich chemiluminescent enzyme immunoassay (CLEIA) to detect thyroid-stimulating hormone (TSH) in a sensitive and specific way. The Therma-Max™ particles allow for automation because, unlike magnetic microspheres, they are completely dispersed in aqueous solution and allow for accurate automatic handling. Signal intensities detected with MNPs were 8-fold higher than those found with conventional micron-sized magnetic particles. A reproducibility study suggests that these particles allow for a stable detection method, as the coefficient of variation (CV) is less than 6% ( n=10).

  1. Red-green-blue electrogenerated chemiluminescence utilizing a digital camera as detector.

    PubMed

    Doeven, Egan H; Barbante, Gregory J; Kerr, Emily; Hogan, Conor F; Endler, John A; Francis, Paul S

    2014-03-01

    Exploiting the distinct excitation and emission properties of concomitant electrochemiluminophores in conjunction with the inherent color selectivity of a conventional digital camera, we create a new strategy for multiplexed electrogenerated chemiluminescence detection, suitable for the development of low-cost, portable clinical diagnostic devices. Red, green and blue emitters can be efficiently resolved over the three-dimensional space of ECL intensity versus applied potential and emission wavelength. As the relative contribution ratio of each emitter to the photographic RGB channels is constant, the RGB ECL intensity versus applied-potential curves could be effectively isolated to a single emitter at each potential. PMID:24512565

  2. Chemiluminescence in autoxidation of hydrocarbons. A method for fingerprinting and evaluation of oxidative stability

    SciTech Connect

    Spllners, I.J.; Hedenburg, J.F.

    1985-09-01

    Chemiluminescence (CL) was generated when mineral oils, lubricants, and synthetic hydrocarbons wer autoxidized at elevated temperature. CL intensity measurements were useful as a rapid method for evaluation of relative oxidative stabilities, and CL spectra served to differentiate and fingerprint hydrocarbon materials. Mineral oils which had been more severely refined to achieve a higher oxidative stability gave lower CL intensity. CL spectra and spectral changes with time were useful to differentiate oils according to their crude sources. CL measurements required less time than the conventional oxidation tests and a good agreement with ASTM D943 oxidation test could be shown. CL was also useful in monitoring and assessing service life left in used lubricants.

  3. Sonochemical fabrication of 8-hydroxyquinoline aluminum (Alq3) nanoflowers with high electrogenerated chemiluminescence.

    PubMed

    Mao, Chang-Jie; Wang, Dan-Chen; Pan, Hong-Cheng; Zhu, Jun-Jie

    2011-03-01

    Well-defined Alq(3) nanoflowers were fabricated via a facile and fast sonochemical route. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to characterize the structure and shape of the as-prepared product. The results showed that the resulting Alq(3) was composed of nanobelts with thickness about 50 nm, average widths of 200 nm, and length up to 10 ?m. The Alq(3) nanoflowers exhibited good electrogenerated chemiluminescence behavior. PMID:20805037

  4. Oscillating chemiluminescence in the system methyl radical plus O[sub 2] plus helium

    SciTech Connect

    Bogan, D.J.; Dongheon Lee; Galanti, M.; Penalosa, M.T. (Catholic Univ. of America, Washington, DC (United States))

    1993-01-07

    Oscillating formaldehyde (S[sub 1] [yields] S[sub 0]) chemiluminescence has been observed in slow laminar flows of methyl radical plus O[sub 2] plus helium at a pressure of 1 atm and temperatures of 508-825 K. All volume elements in the reactor were observed to oscillate synchronously on a 0.01-s time scale, although they had a distribution of residence times on the order of seconds. Period-doubling and period-halving cascades were observed. The oscillatory conditions have been mapped, and a mechanism based upon autocatalytic self-heating is postulated. 22 refs., 4 figs.

  5. Ultrasensitive detection of cancer cells and glycan expression profiling based on a multivalent recognition and alkaline phosphatase-responsive electrogenerated chemiluminescence biosensor

    NASA Astrophysics Data System (ADS)

    Chen, Xiaojiao; He, Yao; Zhang, Youyu; Liu, Meiling; Liu, Yang; Li, Jinghong

    2014-09-01

    A multivalent recognition and alkaline phosphatase (ALP)-responsive electrogenerated chemiluminescence (ECL) biosensor for cancer cell detection and in situ evaluation of cell surface glycan expression was developed on a poly(amidoamine) (PAMAM) dendrimer-conjugated, chemically reduced graphene oxide (rGO) electrode interface. In this strategy, the multivalency and high affinity of the cell-targeted aptamers on rGO provided a highly efficient cell recognition platform on the electrode. The ALP and concanavalin A (Con A) coated gold nanoparticles (Au NPs) nanoprobes allowed the ALP enzyme-catalyzed production of phenols that inhibited the ECL reaction of Ru(bpy)32+ on the rGO electrode interface, affording fast and highly sensitive ECL cytosensing and cell surface glycan evaluation. Combining the multivalent aptamer interface and ALP nanoprobes, the ECL cytosensor showed a detection limit of 38 CCRF-CEM cells per mL in human serum samples, broad dynamic range and excellent selectivity. In addition, the proposed biosensor provided a valuable insight into dynamic profiling of the expression of different glycans on cell surfaces, based on the carbohydrates recognized by lectins applied to the nanoprobes. This biosensor exhibits great promise in clinical diagnosis and drug screening.A multivalent recognition and alkaline phosphatase (ALP)-responsive electrogenerated chemiluminescence (ECL) biosensor for cancer cell detection and in situ evaluation of cell surface glycan expression was developed on a poly(amidoamine) (PAMAM) dendrimer-conjugated, chemically reduced graphene oxide (rGO) electrode interface. In this strategy, the multivalency and high affinity of the cell-targeted aptamers on rGO provided a highly efficient cell recognition platform on the electrode. The ALP and concanavalin A (Con A) coated gold nanoparticles (Au NPs) nanoprobes allowed the ALP enzyme-catalyzed production of phenols that inhibited the ECL reaction of Ru(bpy)32+ on the rGO electrode interface, affording fast and highly sensitive ECL cytosensing and cell surface glycan evaluation. Combining the multivalent aptamer interface and ALP nanoprobes, the ECL cytosensor showed a detection limit of 38 CCRF-CEM cells per mL in human serum samples, broad dynamic range and excellent selectivity. In addition, the proposed biosensor provided a valuable insight into dynamic profiling of the expression of different glycans on cell surfaces, based on the carbohydrates recognized by lectins applied to the nanoprobes. This biosensor exhibits great promise in clinical diagnosis and drug screening. Electronic supplementary information (ESI) available: CV and EIS during the electrode assembly, activity of the nanoprobes and the glycan-binding specificities of the lectins. See DOI: 10.1039/c4nr03053b

  6. Exhaled nitric oxide monitoring by quantum cascade laser: comparison with chemiluminescent and electrochemical sensors

    NASA Astrophysics Data System (ADS)

    Mandon, Julien; Högman, Marieann; Merkus, Peter J. F. M.; van Amsterdam, Jan; Harren, Frans J. M.; Cristescu, Simona M.

    2012-01-01

    Fractional exhaled nitric oxide (FENO) is considered an indicator in the diagnostics and management of asthma. In this study we present a laser-based sensor for measuring FENO. It consists of a quantum cascade laser (QCL) combined with a multi-pass cell and wavelength modulation spectroscopy for the detection of NO at the sub-part-per-billion by volume (ppbv, 1?10-9) level. The characteristics and diagnostic performance of the sensor were assessed. A detection limit of 0.5 ppbv was demonstrated with a relatively simple design. The QCL-based sensor was compared with two market sensors, a chemiluminescent analyzer (NOA 280, Sievers) and a portable hand-held electrochemical analyzer (MINO®, Aerocrine AB, Sweden). FENO from 20 children diagnosed with asthma and treated with inhaled corticosteroids were measured. Data were found to be clinically acceptable within 1.1 ppbv between the QCL-based sensor and chemiluminescent sensor and within 1.7 ppbv when compared to the electrochemical sensor. The QCL-based sensor was tested on healthy subjects at various expiratory flow rates for both online and offline sampling procedures. The extended NO parameters, i.e. the alveolar region, airway wall, diffusing capacity, and flux were calculated and showed a good agreement with the previously reported values.

  7. Exhaled nitric oxide monitoring by quantum cascade laser: comparison with chemiluminescent and electrochemical sensors.

    PubMed

    Mandon, Julien; Högman, Marieann; Merkus, Peter J F M; van Amsterdam, Jan; Harren, Frans J M; Cristescu, Simona M

    2012-01-01

    Fractional exhaled nitric oxide (F(E)NO) is considered an indicator in the diagnostics and management of asthma. In this study we present a laser-based sensor for measuring F(E)NO. It consists of a quantum cascade laser (QCL) combined with a multi-pass cell and wavelength modulation spectroscopy for the detection of NO at the sub-part-per-billion by volume (ppbv, 110(-9)) level. The characteristics and diagnostic performance of the sensor were assessed. A detection limit of 0.5 ppbv was demonstrated with a relatively simple design. The QCL-based sensor was compared with two market sensors, a chemiluminescent analyzer (NOA 280, Sievers) and a portable hand-held electrochemical analyzer (MINO, Aerocrine AB, Sweden). F(E)NO from 20 children diagnosed with asthma and treated with inhaled corticosteroids were measured. Data were found to be clinically acceptable within 1.1 ppbv between the QCL-based sensor and chemiluminescent sensor and within 1.7 ppbv when compared to the electrochemical sensor. The QCL-based sensor was tested on healthy subjects at various expiratory flow rates for both online and offline sampling procedures. The extended NO parameters, i.e. the alveolar region, airway wall, diffusing capacity, and flux were calculated and showed a good agreement with the previously reported values. PMID:22352669

  8. A streptavidin functionalized graphene oxide/Au nanoparticles composite for the construction of sensitive chemiluminescent immunosensor.

    PubMed

    Yang, Zhanjun; Luo, Shufen; Li, Juan; Shen, Juan; Yu, Suhua; Hu, Xiaoya; Dionysiou, Dionysios D

    2014-08-11

    In this work, a novel streptavidin functionalized graphene oxide/Au nanoparticles (streptavidin/GO/AuNPs) composite is prepared and for the first time used to construct sensitive chemiluminescent immunosensor for the detection of tumor marker. The streptavidin/GO/AuNPs composite and the immunosensor are characterized using scanning electron microscopy, static water contact angle measurement and electrochemical impedance spectroscopy. The biofunctionalized composite has large reactive surface area and excellent biocompatibility, thus the capture antibody can be efficiently immobilized on its surface based on the highly selective recognition of streptavidin to biotinylated antibody. Using ?-fetoprotein (AFP) as a model, the proposed chemiluminescent immunosensor shows a wide linear range from 0.001 to 0.1 ng mL(-1) with an extremely low detection limit down to 0.61 pg mL(-1). The resulting AFP immunosensor shows high detection sensitivity, fast assay speed, acceptable detection and fabrication reproducibility, good specificity and stability. The assay results of serum samples with the proposed method are in an acceptable agreement with the reference values. This work provides a promising biofunctionalized nanostructure for sensitive biosensing applications. PMID:25066720

  9. Microplate-reader method for the rapid analysis of copper in natural waters with chemiluminescence detection

    PubMed Central

    Durand, Axel; Chase, Zanna; Remenyi, Tomas; Quéroué, Fabien

    2013-01-01

    We have developed a method for the determination of copper in natural waters at nanomolar levels. The use of a microplate-reader minimizes sample processing time (~25 s per sample), reagent consumption (~120 ?L per sample), and sample volume (~700 ?L). Copper is detected by chemiluminescence. This technique is based on the formation of a complex between copper and 1,10-phenanthroline and the subsequent emission of light during the oxidation of the complex by hydrogen peroxide. Samples are acidified to pH 1.7 and then introduced directly into a 24-well plate. Reagents are added during data acquisition via two reagent injectors. When trace metal clean protocols are employed, the reproducibility is generally less than 7% on blanks and the detection limit is 0.7 nM for seawater and 0.4 nM for freshwater. More than 100 samples per hour can be analyzed with this technique, which is simple, robust, and amenable to at-sea analysis. Seawater samples from Storm Bay in Tasmania illustrate the utility of the method for environmental science. Indeed other trace metals for which optical detection methods exist (e.g., chemiluminescence, fluorescence, and absorbance) could be adapted to the microplate-reader. PMID:23335917

  10. Aroclor 1254 inhibits the chemiluminescence response of peritoneal cavity cells from sharpsnout sea bream (Diplodus puntazzo).

    PubMed

    Vazzana, Mirella; Reas, Gabriele; Cammarata, Matteo; Arizza, Vincenzo; Ferrantelli, Vincenzo; Parrinello, Nicolò

    2014-08-01

    Chronic exposure to polychlorinated biphenyls (PCBs) affect the immune system of fish and could lead to a decreased disease resistance. The effects of Aroclor 1254, PCB mixtures, on the Diplodus puntazzo innate immunity were examined by assaying the zymosan stimulated chemiluminescence response (CL) of peritoneal cavity cells (PCCs) at various times (1, 24, 48 h and 1-4 weeks) from intraperitoneal injection of the xenobiotic (1 mg kg(-1) body weight). Controls were performed by assaying cells from medium-treated fish. Since the kinetic of the chemiluminescence response showed the highest peak at 25 min after the zymosan stimulation of the cells, the values found at that time were considered. The CL enhancement observed at 1 h after the treatment with xenobiotic was followed by a decreased response at 24 h and appeared to be lower at 1-4 weeks when compared to the CL response of the control, suggesting a protracted effect of PCBs on the peritoneal cavity. Since PCCs incubated in vitro for 1 h with 0.05 and 0.1 ?g ml(-1) Aroclor showed an enhanced CL, the effect of the xenobiotic could be exerted on the cell responsiveness to zymosan. It is known that fish CL response of PCCs can be imputed to phagocyte (macrophages and neutrophils) activation, these cells and their responsiveness to zymosan can be used in immunotoxicology assay to monitor the fish health in polluted environment. PMID:24945575

  11. Amorphous carbon nanoparticle used as novel resonance energy transfer acceptor for chemiluminescent immunoassay of transferrin.

    PubMed

    Gao, Hongfei; Wang, Wenwen; Wang, Zhenxing; Han, Jing; Fu, Zhifeng

    2014-03-28

    Amorphous carbon nanoparticles (ACNPs) showing highly efficient quenching of chemiluminescence (CL) were prepared from candle soot with a very simple protocol. The prepared ACNP was employed as the novel energy acceptor for a chemiluminescence resonance energy transfer (CRET)-based immunoassay. In this work, ACNP was linked with transferrin (TRF), and horseradish peroxidase (HRP) was conjugated to TRF antibody (HRP-anti-TRF). The immunoreaction rendered the distance between the ACNP acceptor and the HRP-catalyzed CL emitter to be short enough for CRET occurring. In the presence of TRF, this antigen competed with ACNP-TRF for HRP-anti-TRF, thus led to the decreased occurrence of CRET. A linear range of 20-400 ng mL(-1) and a limit of detection of 20 ng mL(-1) were obtained in this immunoassay. The proposed method was successfully applied for detection of TRF levels in human sera, and the results were in good agreement with ELISA method. Moreover, the ACNPs show higher energy transfer efficiency than other conventional nano-scaled energy acceptors such as graphene oxide in CRET assay. It is anticipated that this approach can be developed for determination of other analytes with low cost, simple manipulation and high specificity. PMID:24636417

  12. Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Botulinum neurotoxins (BoNTs) are some of the most potent biological toxins. High-affinity monoclonal antibodies (mAbs) have been developed for the detection of BoNT serotypes A and B using a chemiluminescent capture enzyme-linked immunosorbent assay (ELISA). In an effort to improve toxin detection ...

  13. Evaluation of the Vitros Syphilis TPA Chemiluminescence Immunoassay as a First-Line Method for Reverse Syphilis Screening.

    PubMed

    González, Victoria; Fernández, Gema; Dopico, Eva; Margall, Nuria; Esperalba, Juliana; Muñoz, Carme; Castro, Elisabeth; Sulleiro, Elena; Matas, Lurdes

    2015-04-01

    We report here the results of the diagnostic performances of Vitros Syphilis TPA (a chemiluminescence treponemal assay) compared with those of two treponemal enzyme immunoassays and of traditional versus reverse syphilis algorithms. Ease of use, automation, and high throughput make the Vitros Syphilis TPA assay a good choice for syphilis screening in high-volume laboratories. PMID:25609729

  14. Chemiluminescence: measuring methods. January 1970-November 1987 (citations from the NTIS data base). Report for January 1970-November 1987

    SciTech Connect

    Not Available

    1987-12-01

    This bibliography contains citations concerning the chemiluminescence analysis of substances. Sample system design, sample collection methods, measurement techniques, and sensitivity of the instrumentation are discussed. High-altitude air-pollution studies are emphasized. (This updated bibliography contains 232 citations, 16 of which are new entries to the previous edition.)

  15. Indole-3-acetic acid biosensor based on G-rich DNA labeled AuNPs as chemiluminescence probe coupling the DNA signal amplification

    NASA Astrophysics Data System (ADS)

    Hun, Xu; Mei, Zhenghua; Wang, Zhouping; He, Yunhua

    2012-09-01

    A highly sensitive chemiluminescence (CL) method for detection of phytohormone indole-3-acetic acid (IAA) was developed by using G-rich DNA labeled gold nanoparticles (AuNPs) as CL probe coupling the DNA signal amplification technology. The IAA antibody was immobilized on carboxyl terminated magnetic beads (MBs). In the presence of IAA, antibody labeled AuNPs were captured by antibody functionalized MBs. The DNA on AuNPs is released by a ligand exchange process induced by the addition of DTT. The released DNA is then acted as the linker and hybridized with the capture DNA on MBs and probe DNA on AuNPs CL probe. The CL signal is obtained via the instantaneous derivatization reaction between a specific CL reagent, 3,4,5-trimethoxyl-phenylglyoxal (TMPG), and the G-rich DNA on AuNPs CL probe. IAA can be detected in the concentration range from 0.02 ng/mL to 30 ng/mL, and the limit of detection is 0.01 ng/mL.

  16. Indole-3-acetic acid biosensor based on G-rich DNA labeled AuNPs as chemiluminescence probe coupling the DNA signal amplification.

    PubMed

    Hun, Xu; Mei, Zhenghua; Wang, Zhouping; He, Yunhua

    2012-09-01

    A highly sensitive chemiluminescence (CL) method for detection of phytohormone indole-3-acetic acid (IAA) was developed by using G-rich DNA labeled gold nanoparticles (AuNPs) as CL probe coupling the DNA signal amplification technology. The IAA antibody was immobilized on carboxyl terminated magnetic beads (MBs). In the presence of IAA, antibody labeled AuNPs were captured by antibody functionalized MBs. The DNA on AuNPs is released by a ligand exchange process induced by the addition of DTT. The released DNA is then acted as the linker and hybridized with the capture DNA on MBs and probe DNA on AuNPs CL probe. The CL signal is obtained via the instantaneous derivatization reaction between a specific CL reagent, 3,4,5-trimethoxyl-phenylglyoxal (TMPG), and the G-rich DNA on AuNPs CL probe. IAA can be detected in the concentration range from 0.02 ng/mL to 30 ng/mL, and the limit of detection is 0.01 ng/mL. PMID:22613129

  17. Capillary electrophoresis chemiluminescent detection system equipped with a two-step postcolumn flow interface for detection of some enkephalin-related peptides labeled with acridinium ester.

    PubMed

    Guo, Longhua; Qiu, Bin; Jiang, Yingyan; You, Zuyao; Lin, Jin-Ming; Chen, Guonan

    2008-06-01

    Despite its low equipment cost and simple design, as one of the sensitive detectors for CE, the chemiluminescence (CL) detector was less developed compared to the detectors of MS and LIF. The main reasons were the limitation of CL reagents, the repeatability problems and the relatively low sensitivity compared to LIF. In this paper, a highly sensitive CE-CL detection system was developed for detection of some enkephalin-related peptides labeled with acridinium ester. A new detection interface was designed for CE with CL detection of acridinium ester and its labeled analytes. The interface included two sections: one was used to acidify the capillary outflow so that the corresponding acridinium pseudo-base form can be changed into acridinium ester form by adding excess acid to the system; the other was designed to provide a suitable solution to produce the CL from acridinium ester. The effect factors, such as pH, the concentration of reaction reagents and the flow rates of the reagents, were investigated. The results showed that acridinium ester had similar CL properties in this interface when pH values of CE BGE were changed from 2.0 to 10.8. The interface was used to detect acridinium ester and three acridinium ester-labeled enkephalin-related peptides, the corresponding LODs were found to be in the attomole range. This CL detection system proved to be of high sensitivity, good repeatability, and relatively low cost. PMID:18435494

  18. Optimization of condition for conjugation of enrofloxacin to enzymes in chemiluminescence enzyme immunoassay

    NASA Astrophysics Data System (ADS)

    Yu, Songcheng; Yu, Fei; Zhang, Hongquan; Qu, Lingbo; Wu, Yongjun

    2014-06-01

    In this study, in order to find out a proper method for conjugation of enrofloxacin to label enzymes, two methods were compared and carbodiimide condensation was proved to be better. The results showed that the binding ratio of enrofloxacin and alkaline phosphatase (ALP) was 8:1 and that of enrofloxacin and horseradish peroxidase (HRP) was 5:1. This indicated that conjugate synthesized by carbodiimide condensation was fit for chemiluminescence enzyme immunoassay (CLEIA). Furthermore, data revealed that dialysis time was an important parameter for conjugation and 6 days was best. Buffer to dilute conjugate had little effect on CLEIA. The storage condition for conjugates was also studied and it was shown that the conjugate was stable at 4 °C with no additive up to 30 days. These data were valuable for establishing CLEIA to quantify enrofloxacin.

  19. Electrogenerated chemiluminescence of common organic luminophores in water using an emulsion system.

    PubMed

    Dick, Jeffrey E; Renault, Christophe; Kim, Byung-Kwon; Bard, Allen J

    2014-10-01

    We describe a method to produce electrogenerated chemiluminescence (ECL) in water using a family of highly hydrophobic polycyclic aromatic hydrocarbon (PAH) luminophores and boron dipyrromethene (BODIPY). This method is based on an oil-in-water emulsion system. Various PAHs (rubrene, 9,10-diphenylanthracene, pyrene, or perylene) and BODIPY were trapped in a toluene and tri-n-propylamine mixed oil-in-water emulsion using an ionic liquid as the supporting electrolyte and emulsifier. ECL was observed for all the aforementioned PAHs and BODIPY, and the rubrene and BODIPY emulsion systems showed adequate light to record an ECL spectrum. ECL was also observed using oxalate as the co-reactant, which was dissolved in the aqueous continuous phase. The emulsions were stable for hours and showed a droplet size distribution that ranged from 275 to 764 nm, in accordance with dynamic light scattering data. PMID:25222019

  20. Photoluminescence, redox properties, and electrogenerated chemiluminescence of twisted 9,9'-bianthryls.

    PubMed

    Natarajan, Palani; Schmittel, Michael

    2013-10-18

    To study the dual emission (from locally excited and charge transfer states) of sterically crowded 9,9'-bianthryl (BAHO) and its 10,10'-disubstituted derivatives, namely, 10,10'-dibromo-9,9'-bianthryl (BABR), 10,10'-bis(p-tolylethynyl)-9,9'-bianthryl (BAET), and 10,10'-bis(N,N-diphenyl-4-anilino)-9,9'-bianthryl (BATA) in detail, we probed their photophysical, redox, and electrogenerated chemiluminescence (ECL) responses. Dual emission for all of the molecules was noticed in PL, whereas in ECL only charge transfer emission was observed over a variety of experimental conditions. The PL in nonpolar solvents is significantly influenced by added supporting electrolyte, yielding exclusively charge transfer emission as in ECL. The stability of ECL proved to depend largely on the nature of the substituent, with triarylamine and bromo groups imparting constant ECL intensity over more than 60 cycles. PMID:24028072