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1

Luminol chemiluminescence under interaction with heteropoly acids.  

PubMed

Interaction of luminol with phosphomolybdic, phosphovanadomolybdic and silicomolybdic acids was studied by examination of chemiluminescence spectra, measurement of ESR spectra, investigation of reaction order, and elucidation of inhibition effects. A scheme of the reaction mechanism is proposed. PMID:19269491

Zui, Oleg; Takahashi, Hiroki; Hori, Toshitaka; Hinoue, Teruo

2009-05-15

2

The influence of dioxygen on luminol chemiluminescence.  

PubMed

Assays of peroxy compounds are commonly performed after chromatographic separation of analysed mixtures. In high-performance liquid chromatography (HPLC), solvent reservoirs are sparged by helium or inline vacuum-degassed in order to control the compressibility of the solvents for efficient pumping. In this study, we investigated the influence of degassing the reaction solution on the light output of the hemin-catalyzed luminol oxidation by various oxidants. We found that, when t-butyl hydroperoxide, hydrogen peroxide, n-butyl hydroperoxide, iodosobenzene and iodobenzene diacetate were used as oxidants, the luminol chemiluminescence was lowered by 50-70% compared with an equilibrated and degassed solution. The opposite effect was observed when dibenzoyl peroxide and 3-chloroperoxybenzoic acid were used as oxidants, as the chemiluminescence increased by approximately 20-30%. The reduced chemiluminescence was explained based on the known role of dioxygen in luminol chemiluminescence. The enhancement of chemiluminescence was rationalized by suggesting an alternative mechanism of luminol oxidation valid for peroxyacids and diacyl peroxides in which the reaction of a peroxyacid anion with the diazaquinone led to light emission with a higher quantum yield than the usual path, which is suppressed by the removal of dioxygen from the reaction solution. PMID:19294631

Baj, Stefan; Krawczyk, Tomasz; Staszewska, Karina

2009-01-01

3

Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: application in luminescence-monitored enzyme immunoassays.  

PubMed

Certain phenol derivatives, including p-iodophenol and p-phenylphenol, enhance light emission from the horseradish peroxidase-catalyzed oxidation of cyclic diacyl hydrazides such as luminol. The light emission decays slowly (glowing for several minutes) and its intensity may be greater than 1000-fold that of the unenhanced reaction. The enhanced system enables rapid, sensitive assay of peroxidase conjugates. We describe its application in immunoassays for human choriogonadotropin, digoxin, and factor VIII-related antigen. Luminescent quantification of peroxidase labels has been directly incorporated into immunoassays based on beads, tubes, or microtiter plates, used in conjunction with photodetectors such as photomultiplier tubes or instant photographic film. Enhancement with phenol derivatives exceeds that achieved with 6-hydroxybenzothiazole derivatives and depends on pH and enhancer concentration. Emission spectra of phenol-enhanced and unenhanced reactions are remarkably similar, suggesting that the enhancers do not act as more efficient emitters but exert their action earlier in the complex reaction between peroxidase, oxidant, and luminol. PMID:3926345

Thorpe, G H; Kricka, L J; Moseley, S B; Whitehead, T P

1985-08-01

4

Phenol derivatives as enhancers and inhibitors of luminol-H2O2-horseradish peroxidase chemiluminescence.  

PubMed

Systematic studies on phenol derivatives facilitates an explanation of the enhancement or inhibition of the luminol-H2O2-horseradish peroxidase system chemiluminescence. Factors that govern the enhancement are the one-electron reduction potentials of the phenoxy radicals (PhO./PhOH) vs. luminol radicals (L./LH-) and the reaction rates of the phenol derivatives with the compounds of horseradish peroxidase (HRP-I and HRP-II). Only compounds with radicals with a similar or greater reduction potential than luminol at pH 8.5 (0.8V) can act as enhancers. Radicals with reduction potentials lower than luminol behave in a different way, because they destroy luminol radicals and inhibit chemiluminescence. The relations between the reduction potential, reaction rates and the Hammett constant of the substituent in a phenol suggest that 4-substituted phenols with Hammett constants (sigma) for their substituents similar or greater than 0.20 are enhancers of the luminol-H2O2-horseradish peroxidase chemiluminescence. In contrast, those phenols substituted in position 4 for substituents with Hammett constants (sigma) lower than 0.20 are inhibitors of chemiluminescence. On the basis of these studies, the structure of possible new enhancers was predicted. PMID:9633010

Navas Díaz, A; García Sánchez, F; González Garcia, J A

1998-01-01

5

A flow injection chemiluminescence method for the determination of fluoroquinolone derivative using the reaction of luminol and hydrogen peroxide catalyzed by gold nanoparticles  

Microsoft Academic Search

Based on the enhancement of chemiluminescence (CL) of luminol-hydrogen peroxide-gold nanoparticles system by fluoroquinolones (FQs), a novel and rapid CL method is reported for the determination of FQs derivatives. Under the optimum conditions, the CL intensity is proportional to the concentration of FQs derivative in solution. The corresponding linear regression equations are established over the range of 0.08–1.28?g\\/mL for norfloxacin,

Lun Wang; Ping Yang; Yongxin Li; Hongqi Chen; Maoguo Li; Fabao Luo

2007-01-01

6

Synthesis and Characterization of Luminol Persulphate Chemiluminescence in Aqueous Amines  

NASA Astrophysics Data System (ADS)

The chemiluminescence (CL) emission spectra of luminol were recorded using Fuss spectrograph in different aqueous aliphatic amines using sodium persulphate alone and mixture with hydrogen peroxide as an oxidant. The CL emission spectra after resolution showed two emission bands at 425 and 455 nm. The CL mechanism was explained on the basis of two exited state species formed during oxidation of luminol. The CL of luminol is found to be very weak as persulphate slowly produced oxygen. The glow become intense with time as more and more oxygen is made available for oxidation of luminol. The mixture of hydrogen peroxide and sodium persulphate is found to be more effective in producing intense and long lived CL glow for luminol. The CL emission band of luminol by using sodium persulphate and mixture with hydrogen peroxide is explained on the basis of formation of exited singlet and triplet state of 3-aminophthalate ion (3-APA). The shorter wavelength emission band of 425 nm is found to be very weak in intensity as compared to longer wavelength emission band of 455 nm. Thus phosphoresce is favored in case of persulphate CL of luminol.

Raut, V. M.; More, P. S.; Khollam, Y. B.; Sonone, R. S.; Kondawar, S. B.; Koinkar, Pankaj

7

Studies on the Chemiluminescence of Luminol in Dimethylsulfoxide and Dimethysulfoxide-Water Mixtures.  

National Technical Information Service (NTIS)

The absorption, fluorescence, and chemiluminescence (CL) characteristics of luminol have been studied in basic dimethylsulfoxide (DMSO) and various basic DMSO-water mixed solvents. It has been shown that the luminol dianion can be produced quantitatively ...

D. M. Hercules J. D. Gorsuch

1971-01-01

8

Luminol-silver nitrate chemiluminescence enhancement induced by cobalt ferrite nanoparticles.  

PubMed

CoFe(2)O(4) nanoparticles (NPs) could stimulate the weak chemiluminescence (CL) system of luminol and AgNO(3), resulting in a strong CL emission. The UV-visible spectra, X-ray photoelectron spectra and TEM images of the investigated system revealed that AgNO(3) was reduced by luminol to Ag in the presence of CoFe(2)O(4) NPs and the formed Ag covered the surface of CoFe(2)O(4) NPs, resulting in CoFe(2)O(4)-Ag core-shell nanoparticles. Investigation of the CL reaction kinetics demonstrated that the reaction among luminol, AgNO(3) and CoFe(2)O(4) NPs was fast at the beginning and slowed down later. The CL spectra of the luminol - AgNO(3) - CoFe(2)O(4) NPs system indicated that the luminophor was still an electronically excited 3-aminophthalate anion. A CL mechanism has been postulated. When the CoFe(2)O(4) NPs were injected into the mixture of luminol and AgNO(3), they catalyzed the reduction of AgNO(3) by luminol to produce luminol radicals and Ag, which immediately covered the CoFe(2)O(4) NPs to form CoFe(2)O(4)-Ag core-shell nanoparticles, and the luminol radicals reacted with the dissolved oxygen, leading to a strong CL emission. With the continuous deposition of Ag on the surface of CoFe(2)O(4) NPs, the catalytic activity of the core-shell nanoparticles was inhibited and a decrease in CL intensity was observed and also a slow growth of shell on the nanoparticles. PMID:21400653

Shi, Wenbing; Wang, Hui; Huang, Yuming

2011-01-01

9

Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membrane.  

PubMed

Photographic detection of horseradish peroxidase (HRP) on a membrane by the luminol-hydrogen peroxide-HRP chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid (DPPA) as an enhancer is described. The method is based on the long-lived chemiluminescence emission obtained by using DPPA. Under the optimum conditions, as little as 0.10 ng (ca. 2.3 fmol) and 0.20 ng (ca. 4.6 fmol) per spot of HRP on a membrane were detected as visible spots with exposure time of 60 and 10 min, respectively, by using an instant photographic film and a camera luminometer. The proposed method was highly sensitive and was successfully applied to the detection of HRP conjugates as an alternative to the colorimetric method using a chromogenic substrate in a commercially available assay kit of Western blotting. PMID:11312543

Kuroda, N; Murasaki, N; Wada, M; Nakashima, K

2001-01-01

10

[Human serum albumin modified under oxidative/halogenative stress enhances luminol-dependent chemiluminescence of human neutrophils].  

PubMed

It is shown that human serum albumin, previously treated with HOCl (HSA-Cl), enhances luminol-dependent chemiluminescence of neutrophils activated by phorbol-12-myristate-13-acetate (PMA). The enzyme-linked immunosorbent assay revealed that addition of HSA-Cl to neutrophils promotes exocytosis of myeloperoxidase. Inhibitor of myeloperoxidase--4-aminobenzoic acid hydrazide, without any effect on lucigenin-dependent chemiluminescence of neutrophils stimulated with PMA, effectively suppressed luminol-dependent chemiluminescence (IC50 = 20 microM) under the same conditions. The transfer of the cells from medium with HSA-Cl and myeloperoxidase to fresh medium abolished an increase in PMA-induced luminol-dependent chemiluminescence, but not the ability of neutrophils to respond to re-addition of HSA-Cl. A direct and significant (r = 0.75, p) correlation was observed between the intensity of PMA stimulated neutrophil chemiluminescence response and myeloperoxidase activity in the cell-free media after chemiluminescence measurements. These results suggest the involvement of myeloperoxidase in the increase of neutrophil PMA-stimulated chemiluminescence response in the presence of HSA-Cl. A significant positive correlation was found between myeloperoxidase activity in blood plasma of children with severe burns and the enhancing effects of albumin fraction of the same plasma on luminol-dependent chemiluminescence of PMA-stimulated donor neutrophils. These results support a hypothesis that proteins modified in reactions involving myeloperoxidase under oxidative/halogenative stress, stimulate neutrophils, leading to exocytosis of myeloperoxidase, a key element of halogenative stress, and to closing a "vicious circle" of neutrophil activation at the inflammatory site. PMID:24455888

Mikhal'chik, E V; Smolina, N V; Astamirova, T C; Gorudko, I V; Grigor'eva, D V; Ivanov, V A; Sokolov, A V; Kostevich, V A; Cherenkevich, S N; Panasenko, O M

2013-01-01

11

A flow injection chemiluminescence method for the determination of fluoroquinolone derivative using the reaction of luminol and hydrogen peroxide catalyzed by gold nanoparticles.  

PubMed

Based on the enhancement of chemiluminescence (CL) of luminol-hydrogen peroxide-gold nanoparticles system by fluoroquinolones (FQs), a novel and rapid CL method is reported for the determination of FQs derivatives. Under the optimum conditions, the CL intensity is proportional to the concentration of FQs derivative in solution. The corresponding linear regression equations are established over the range of 0.08-1.28mug/mL for norfloxacin, 0.013-1.32mug/mL for ciprofloxacin, 0.014-1.4mug/mL for lomefloxacin, 0.029-1.46mug/mL for fleroxacin, 0.02-1.0mug/mL for ofloxacin and 0.01-1.44mug/mL for levofloxacin, respectively. The limits of detection (S/N=3) are 3.2, 9.5, 7.0, 9.0, 8.0, and 8.0ng/mL with the relative standard deviation (n=11) 4.3, 1.5, 1.9, 1.3, 1.6 and 2.1% for norfloxacin, ciprofloxacin, lomefloxacin, fleroxacin, ofloxacin and levofloxacin, respectively. This proposed method has been applied to detect FQs derivatives in human urine successfully. PMID:19071726

Wang, Lun; Yang, Ping; Li, Yongxin; Chen, Hongqi; Li, Maoguo; Luo, Fabao

2007-05-15

12

Flow-injection determination of isoniazid using sodium dichloroisocyanurate- and trichloroisocyanuric acid-luminol chemiluminescence systems  

Microsoft Academic Search

A chemiluminescent (CL) method for the determination of isoniazid is described. The method is based on the CL generated during the oxidation of luminol by sodium dichloroisocyanurate (SDCC) and trichloroisocyanuric acid (TCCA) in alkaline medium. It was found that isoniazid greatly enhances this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method

A Safavi; M. A Karimi; M. R Hormozi Nezhad

2004-01-01

13

Comparison of 5Hydroxy2, 3-Dihydrophthalazine-1, 4Dione and Luminol as Co-Substrates for Detection of Horseradish Peroxidase in Enhanced Chemiluminescent Reactions  

Microsoft Academic Search

The utility of 5-hydroxy-2, 3-dihydrophthalazine-1, 4-dione (HDP) as a co-substrate for the chemiluminescent detection of horseradish peroxidase was assessed. Several substituted aryl boronic acid derivatives (4-phenyl, 4-iodo) acted as potent enhancers of the peroxidase catalyzed reaction. Addition of chelating agents (EDTA) and surfactants (Tween-20 and [poly (vinylbenzyl)tributylphosphonium chloride-poly (vinylbenzyl) trioctylphosphonium chloride copolymer]) modulated background light emission and the intensity and

Larry J. Kricka; Xiaoying Ji; Gary H. G. Thorpe; Brooks Edwards; John Voyta; Irena Bronstein

1996-01-01

14

Chemiluminescence determination of surfactant Triton X-100 in environmental water with luminol-hydrogen peroxide system  

PubMed Central

Background The rapid, simple determination of surfactants in environmental samples is essential because of the extensive use and its potential as contaminants. We describe a simple, rapid chemiluminescence method for the direct determination of the non-ionic surfactant Triton X-100 (polyethylene glycol tert-octylphenyl ether) in environmental water samples. The optimized experimental conditions were selected, and the mechanism of the Luminol-H2O2-Triton X-100 chemiluminesence system was also studied. Results The novel chemiluminescence method for the determination of non-ionic surfactant Triton X-100 was based on the phenomenon that Triton X-100 greatly enhanced the CL signal of the luminol-H2O2 system. The alkaline medium of luminol and the pH value obviously affected the results. Luminol concentration and hydrogen peroxide concentration also affected the results. The optimal conditions were: Na2CO3 being the medium, pH value 12.5, luminol concentration 1.0 × 10-4 mol L-1, H2O2 concentration 0.4 mol L-1. The possible mechanism was studied and proposed. Conclusion Under the optimal conditions, the standard curve was drawn up and quotas were evaluated. The linear range was 2 × 10-4 g·mL-1-4 × 10-2 g·mL-1 (w/v), and the detection limit was 3.97 × 10-5 g·mL-1 Triton X-100 (w/v). The relative standard deviation was less than 4.73% for 2 × 10-2 g·mL-1 (w/v) Triton X-100 (n = 7). This method has been applied to the determination of Triton X-100 in environmental water samples. The desirable recovery ratio was between 96%–102% and the relative standard deviation was 2.5%–3.3%. The luminescence mechanism was also discussed in detail based on the fluorescence spectrum and the kinetic curve, and demonstrated that Triton X-100-luminol-H2O2 was a rapid reaction.

Liu, Xiaoyu; Li, Aifang; Zhou, Baohui; Qiu, Chaokun; Ren, Hongmin

2009-01-01

15

Luminol-enhanced chemiluminescence induced in peripheral blood-derived human phagocytes: obligatory requirement of myeloperoxidase exocytosis by monocytes  

Microsoft Academic Search

Luminol-enhanced chemiluminescence (LCL) of phagocytes is often used to monitor for the generation of reactive oxygen mediators. A strong LCL reaction, as observed in triggered peripheral blood monocytes and neutrophils, depends on both the activation of an NAD(P)H-dependent oxidase and a functional myeloper- oxidase (MP0). The aim of this work was to compare the LCL response induced by soluble and

D. Albrecht; T. W. Jungi

16

Trace analysis of phosphorus in water by sorption preconcentration and luminol chemiluminescence  

PubMed

A new, highly sensitive chemiluminescence method for the determination of sub-ppb quantities of phosphorus in water is described. The method is based on sorption preconcentration of phosphorus as a yellow vanadomolybdophosphoric heteropoly acid (HPA) in the presence or absence of a cationic surfactant on a paper filter, followed by direct chemiluminescence detection of the phosphorus concentrate via reaction with an alkaline luminol solution. The molar ratio of cationic surfactant to HPA in the ion associate sorbed on the filter is 4:1. The detection limits for phosphorus are 0.02 microgram of P L-1 in the presence of surfactant and 0.1 microgram of P L-1 in the absence of surfactant for a sample volume of 150 mL. The calibration plot is linear from 0.06 to 1.7 micrograms of P L-1 in the presence of a surfactant, and the time required for analysis is 25 min. In the absence of surfactant, the selectivities against Si4+ and As5+ are 5 and 40 times greater than those for the standard colorimetric method based on the formation of the blue molybdophosphoric HPA. Applications of the method to the analyses of river water, seawater, and the turbine vapor condensate from a coal-fired power plant are described. It is demonstrated that the sensitivity advantage of the chemiluminescence technique can be combined with the magnesium-induced coprecipitation (MAGIC) method for a more selective measurement of soluble reactive phosphorus. PMID:10763271

Zui; Birks

2000-04-01

17

Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere  

NASA Astrophysics Data System (ADS)

An instrument has been designed and constructed for the simultaneous determination of nitrogen dioxide (NO2) and peroxyacetyl nitrate (PAN) in atmospheric samples. The instrument's design is based on separation by fast gas chromatography (GC) with a 30 ft capillary column (DB-1) followed by detection by luminol chemiluminescence. The chemiluminescent reaction between NO2 or PAN and luminol takes place at the gas-liquid interface on the surface of a solid support. The chemiluminescent emission at 425 nm is detected with a photon counting module. The instrument is controlled by a 1.8 GHz Notebook computer with a WINDOWS 2000 operating system and a custom software application programmed in LABVIEW. Detection limits are in the low parts per trillion (ppt) with a time resolution of 30 s to 1 min. The instrument was operated during the Mexico City Metropolitan Area/Mexico City Megacity 2003 collaborative air quality study. Results for NO2 from this fast GC method were compared with results from a co-located differential optical absorption spectrometer (DOAS) and a tunable diode laser absorption spectromenter (TDLAS). The results support the application of the new luminol-based instrument for atmospheric measurements.

Marley, Nancy A.; Gaffney, Jeffrey S.; White, Robert V.; Rodriguez-Cuadra, Luis; Herndon, Scott E.; Dunlea, Ed; Volkamer, Rainer M.; Molina, Luisa T.; Molina, Mario J.

2004-11-01

18

Assay of picogram level isocarbophos residue on tangerines and oranges with luminol-albumin chemiluminescence system.  

PubMed

A sensitive flow injection-chemiluminescence (FI-CL) method for the determination of isocarbophos (ICP) residue on tangerines and oranges was proposed. It was found that the CL intensity from luminol-albumin CL reaction could be obviously quenched in the presence of ICP and the decrease in CL intensity was proportional to the logarithm of ICP concentrations ranging from 1.0 to 1000 pmol L(-1), giving the limit of detection of 0.3 pmol L(-1) (3?). The proposed procedure was successfully applied to the determination of ICP residue on tangerines and oranges with recoveries varying from 92.0 to 111.0% and RSDs less than 5.0%. The possible CL mechanism of luminol-albumin-ICP reaction was discussed, and ICP to albumin's binding constant (K(D)=1.00 × 10(6) L mol(-1)) and the number of binding sites (n=1.00) were given by the homemade FI-CL model. PMID:22980841

Chen, Donghua; Song, Zhenghua; Lv, Hairu

2012-12-15

19

Effect of gold nanoparticle as a novel nanocatalyst on luminol-hydrazine chemiluminescence system and its analytical application.  

PubMed

In this work the catalytic role of unsupported gold nanoparticles on the luminol-hydrazine reaction is investigated. Gold nanoparticles catalyze the reaction of hydrazine and dissolved oxygen to generate hydrogen peroxide and also catalyze the oxidation of luminol by the produced hydrogen peroxide. The result is an intense chemiluminescence (CL) due to the excited 3-aminophthalate anion. In the absence of gold nanoparticles no detectable CL was observed by the reaction of luminol and hydrazine unless an external oxidant is present in the system. The size effect of gold nanoparticles on the CL intensity was investigated. The most intensive CL signals were obtained with 15-nm gold nanoparticles. UV-vis spectra and transmission electron microscopy studies were used to investigate the CL mechanism. The luminol and hydroxide ion concentration, gold nanoparticles size and flow rate were optimized. The proposed method was successfully applied to the determination of hydrazine in boiler feed water samples. Between 0.1 and 30 microM of hydrazine could be determined with a detection limit of 30 nM. PMID:18291135

Safavi, A; Absalan, G; Bamdad, F

2008-03-10

20

Chemiluminescence of Luminol in the Presence of Hematin Compounds.  

National Technical Information Service (NTIS)

A rapid and sensitive method has been described for the determination of micro amounts of hematin iron. The assay is based on the luminescence of alkaline luminol in the presence of hematin iron and hydrogen peroxide. The assay system employs the direct r...

H. A. Neufeld C. J. Conklin R. D. Towner

1965-01-01

21

Flow-injection determination of isoniazid using sodium dichloroisocyanurate- and trichloroisocyanuric acid-luminol chemiluminescence systems.  

PubMed

A chemiluminescent (CL) method for the determination of isoniazid is described. The method is based on the CL generated during the oxidation of luminol by sodium dichloroisocyanurate (SDCC) and trichloroisocyanuric acid (TCCA) in alkaline medium. It was found that isoniazid greatly enhances this CL intensity when present in the luminol solution. Based on this observation, a new flow-injection CL method for the determination of isoniazid has been proposed in this paper. The detection limits were 2 and 3 ng ml(-1) isoniazid for the SDCC-luminol and TCCA-luminol CL systems, respectively. The relative CL intensity was linear with the isoniazid concentration in the range of 4-100 and 100-200 ng ml(-1) for the SDCC-luminol CL system, and 6-200 and 200-1000 ng ml(-1) for the TCCA-luminol CL system. The results obtained for the assay of pharmaceutical preparations compared well with those obtained by the official methods and demonstrated good accuracy and precision. PMID:15178311

Safavi, A; Karimi, M A; Hormozi Nezhad, M R

2004-06-01

22

Luminol-labeled gold nanoparticles for ultrasensitive chemiluminescence-based chemical analyses.  

PubMed

We report a study on chemiluminescence-based chemical analyses using luminol molecules covalently attached to 10 nm diameter gold nanoparticles (GNPs). Chemiluminescence (CL) has been systematically studied under two schemes by varying the concentrations of luminol-labeled GNPs and [Fe(CN)6](3-) catalyst, respectively. The CL signal of luminol-labeled GNPs is enhanced by 5 to 10 times compared to the bulk luminol solutions of the same concentration. The log-log plot of the CL signal versus the number of luminol-labeled GNPs suspended in a standard 96-well plate shows two characteristic linear curves with distinct slopes across eight orders of magnitude variation in the GNP quantity (from 1.82 × 10(2) to 1.82 × 10(10) GNPs per well). The detection limit represented by the cross-point of these two curves can reach down to ~6.1 × 10(5) GNPs per well (corresponding to 1.0 × 10(-14) M GNP and 2.4 × 10(-11) M equivalent luminol concentration). The attachment of luminol molecules to GNP nano-carriers allows a large amount of luminol to be placed in a greatly reduced volume (or area) toward developing miniaturized CL sensors. We have demonstrated this by preloading dried luminol-labeled GNPs in homemade microwell arrays (with a volume of ~12 ?L per well). A linear log-log curve can be obtained across the full range from 1 × 10(3) to 1 × 10(10) GNPs per microwell. The CL signal was detectable with as few as ~1000 GNPs. We have further applied this microwell method to the detection of highly diluted blood samples, in both intact and lysed forms, which releases Fe(3+)-containing hemoglobin to catalyze luminol CL. The lysed blood sample can be detected even after a 10(8) fold dilution (corresponding to ~0.18 cells per well). This ultrasensitive CL detection method may be readily adapted for developing various miniaturized multiplex biosensors for rapid chemical/biochemical analyses. PMID:23851397

Syed, Lateef U; Swisher, Luxi Zhang; Huff, Hannah; Rochford, Caitlin; Wang, Fengli; Liu, Jianwei; Wu, Judy; Richter, Mark; Balivada, Sivasai; Troyer, Deryl; Li, Jun

2013-10-01

23

Droplet detector for the continuous flow luminol-hydrogen peroxide chemiluminescence system.  

PubMed

A droplet detector with high sensitivity and low consumption of reagents and sample for continuous flow chemiluminescence (CL) detection has been developed. The proposed system directly mixes sample with luminescence reagents to form a small droplet, thanks to the gravity force and surface tension of water, in front of a photomultiplier tube (PMT) where the CL reaction takes place. The PMT can then instantaneously record the maximum CL light signal emitted from the whole reaction process. This small droplet can replace the conventional spiral-shaped flow cell for flow injection CL analysis and the droplet detector can reduce interference from the reaction products and residual reagents. To evaluate the potential application of the proposed detector, the CL detection of Fe(ii) was assessed under the alkaline luminol-hydrogen peroxide conditions. The CL intensity increased linearly with the concentration of Fe(ii) ranging from 10.0 to 1000 nmol dm(-3) with a detection limit of 7.16 nmol dm(-3) (S/N = 3). The droplet detector allows highly sensitive, stable and fast CL detection of analytes, and thus is promising for application to other analytes having fast CL reaction rates. PMID:19173062

Wen, Yaqiong; Yuan, Hongyan; Mao, Jianfei; Xiao, Dan; Choi, Martin M F

2009-02-01

24

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses.  

PubMed

Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields. PMID:24752935

Khan, Parvez; Idrees, Danish; Moxley, Michael A; Corbett, John A; Ahmad, Faizan; von Figura, Guido; Sly, William S; Waheed, Abdul; Hassan, Md Imtaiyaz

2014-05-01

25

Effects of pteridines on luminol-dependent chemiluminescence induced by chloramine-T  

Microsoft Academic Search

Pteridines are ubiquitous in living organisms, but little is known about their biological functions. Different pteridines were tested for their ability to modulate luminol-dependent chemiluminescence induced by chloramine-T at pH = 7.5 and at a concentration of 100 ?M for each pteridine. We observed striking differences between the compounds; whereas reduced pteridine species were generally potent scavengers, aromatic pteridines were

Gilbert Reibnegger; Dietmar Fuchs; Christian Murr; Manfred P. Dierich; Wolfgang Pfleiderer; Helmut Wachter

1995-01-01

26

A Myeloperoxidase-Specific Assay Based upon Bromide-Dependent Chemiluminescence of Luminol  

Microsoft Academic Search

Measurement of myeloperoxidase (MPO; EC 1.11.1.7) activity is often used as a marker of neutrophil infiltration into tissues. However, most enzymatic assays for MPO are susceptible to interference from other peroxidases (including eosinophil peroxidase, EPX) and hemoproteins (such as hemoglobin and myoglobin) present in the tissues. In this report, we describe a bromide-dependent chemiluminescence (Br-CL) assay that uses luminol as

Arsalan S. Haqqani; Jagdeep K. Sandhu; H. Chaim Birnboim

1999-01-01

27

Spontaneous Luminol-Dependent Chemiluminescence of Thrombocytes Increases in Hemorrhagic Fever with Renal Syndrome  

Microsoft Academic Search

Spontaneous luminol-dependent chemiluminescence (CL) of thrombocytes was studied in 12 patients with hemorrhagic fever with renal syndrome (HFRS) caused by Puumala virus. CL in the acute phase of HFRS (median, 61 mV\\/10 sec; range, 22-91) was higher than that of healthy controls (median, 22 mV\\/10 sec; range, 18-29; P < 0.01) and patients with acute hepatitis B infection accompanied by

Oleg A. Alexeyev; Tatjana V. Suzdaltseva; Vjacheslav G. Morozov; Alexander V. Zhestkov; Andrei S. Mishukov

1995-01-01

28

Investigation of the radical step in the chemiluminescent oxidation of luminol by potassium ferricyanide in the presence of hydrogen peroxide with the aid of magnetic modulation  

Microsoft Academic Search

A method employing magnetic modulation of the rate of the chemical reaction was previously applied with success to the establishment of the chemiluminescent reaction involving the oxidation of luminol (LHâ) by potassium ferricyanide KâFe-(CN)â in an aqueous solution of sodium hydroxide (0.1 M NaOH). The purpose of the present work was to experimentally investigate the radical steps of the more

M. M. Tribel; A. K. Morozov; E. L. Frankevich

1987-01-01

29

Vitamin A determination in milk samples based on the luminol-periodate chemiluminescence system.  

PubMed

A simple and rapid flow injection (FI) method for the determination of retinyl acetate is reported based on its enhancing effect on the luminol-periodate chemiluminescence (CL) system in an alkaline medium. The detection limit (3s×blank) was 8.0×10?? mol L?¹, with an injection throughput of 90 h?¹. The method allows linear increase of CL intensity over the retinyl acetate concentration range of 1.0-100×10?? mol L?¹ (R²=0.9996) with relative standard deviations of 2.4% (n=10) for 5.0×10?? mol L?¹. The key chemical and physical variables (reagent concentrations, flow rates, sample volume, and photomultiplier tube (PMT) voltage) were optimized and potential interferences were investigated. The method was successfully applied to human milk, fresh cow's milk and infant milk-based formulas and the results were in good agreement with the previously reported HPLC method. A brief discussion on the possible CL reaction mechanism is also presented. PMID:24759254

Rishi, Lubna; Yaqoob, Mohammad; Waseem, Amir; Nabi, Abdul

2014-01-01

30

Effects of pH and Surfactant on the Ultrasound-Induced Chemiluminescence of Luminol  

NASA Astrophysics Data System (ADS)

The pH dependence of the sonochemi-luminescence (SCL) of luminol at 141 kHz was investigated and compared to the effect of pH on its fluorescence emission intensity. From the fluorescence intensity, the pKa1* and the pKa2* values were determined to be 6.4 and 10.5 respectively, which was consistent with changes observed in the SCL intensity of luminol when the pH was varied. An inverse relationship was found to exist between the SCL and the fluorescence intensities against pH, i.e., the luminol SCL spectrum at pH=10.8 (mono-anionic form) had the same shape as the fluorescence emission spectra in acidic pH solutions, where luminol exists in either the mono-cationic or neutral form. The addition of an anionic surfactant, sodium dodecyl sulfate (SDS), significantly decreased the luminol SCL intensity at high pH@. It is proposed that the generation of a negative electric potential at the surface of cavitation bubbles effectively prevented the negatively charged form of luminol from approaching the bubbles, thereby inhibiting the reaction of luminol with OH radicals.

Miyoshi, Norio; Hatanaka, Shin-ichi; Yasui, Kyuichi; Mitome, Hideto; Fukuda, Masaru

2001-06-01

31

Enhanced chemiluminescence in the peroxidase-luminol-H2O2 system: anomalous reactivity of enhancer phenols with enzyme intermediates.  

PubMed

Phenols which markedly enhance chemiluminescence in the horseradish peroxidase catalysed oxidation of luminol by hydrogen peroxide show anomalously high reactivity (by factors of approximately 10(2) compared with published Hammett correlations) in the reduction of the enzyme intermediates, Compound I and Compound II. The results support the hypothesis that efficient production of phenoxy radicals from phenols is a necessary criterion for chemiluminescence enhancer action. PMID:2711890

Hodgson, M; Jones, P

1989-01-01

32

Sodium azide as a specific quencher of singlet oxygen during chemiluminescent detection by luminol and Cypridina luciferin analogues.  

PubMed

Reactive oxygen species (ROS) are presently thought to play important role in an increasing number of the physiological and pathological processes in living organisms. Various chemiluminescent (CL) compounds have been studied in order to find suitable and specific probes for the detection of particular ROS species. The CL of luminol is known to be non-specific and can be induced by various oxidants. Two Cypridina luciferin analogues, CLA and MCLA, have been used for the detection of ROS in vivo. CLAs are thought to emit light only when reacting with superoxide and singlet oxygen. It is possible to distinguish the particular ROS by using a specific quencher or scavenger, e.g. superoxide dismutase (SOD) or sodium azide (NaN(3)). The CL reactions of luminol (3-aminophthalhydrazide), CLA [2-methyl-6-phenyl-3,7-dihydroimidazo(1,2?) pyrazin-3-one] and MCLA [2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo(1,2?) pyrazin-3-one] were studied in three hydrogen peroxide decomposition systems (H(2)O(2)-HRP; H(2)O(2)-CuSO(4); and H(2)O(2)-NaOCl). The measurements were carried out in phosphate buffer, pH 7.4, at 25°C, using a luminometer (Fluoroskan Ascent FL and Sirius C). NaN(3) was used as the specific quencher of singlet oxygen. The results demonstrate that the proclaimed specificity of the CL of Cypridina luciferin analogues towards singlet oxygen has to be discussed. PMID:21491580

Bancirova, Martina

2011-01-01

33

Investigation of Chemiluminescent Reactions.  

National Technical Information Service (NTIS)

The utilization of aqueous environments as media for peroxyoxalate chemiluminescence of rubrenesulfonate was further explored. The fluorescence and chemiluminescence of rubrenesulfonate was found to increase with the addition of Deceresol NI, a nonionic s...

B. H. Baretz W. J. Trzaskos L. Elliott D. L. Dauplaise R. R. Hautala

1984-01-01

34

Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate.  

PubMed

In this study, a high sensitivity chemiluminescence enzyme immunoassay (CLEIA) based on novel enhancers was developed. Under optimal conditions, we developed an enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP-C) in the presence of 3-(10'-phenothiazinyl) propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP) as enhancers. The limit of detection of the newly prepared chemiluminescent cocktail for HRP was 0.33?pg/well, which is lower than that of commercial Super Signal substrate. The results showed that this novel chemiluminescent cocktail can significantly increase the light output of HRP-catalyzed ECR, which can be translated into a corresponding improvement in sensitivity. Similar improvements were observed in CLEIA for the determination of chloramphenicol in milk. In addition, the ECR of N-azoles as secondary enhancer was also presented. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23785024

Tao, Xiaoqi; Wang, Wenjun; Wang, Zhanhui; Cao, Xingyuan; Zhu, Jinghui; Niu, Lanlan; Wu, Xiaoping; Jiang, Haiyang; Shen, Jianzhong

2014-06-01

35

Effect of aggregated silver nanoparticles on luminol chemiluminescence system and its analytical application  

NASA Astrophysics Data System (ADS)

We found that after silver nanoparticles (AgNPs) aggregated, its catalytic activity on luminol CL reaction obviously changed, and the change characteristic was closely related to the sizes of AgNPs. UV-visible spectra, X-ray photoelectron spectra, zeta potential and transmission electron microscopy studies were carried out to investigate the CL effect mechanism. The different CL responses of aggregated AgNPs with different size were suggested to be due to the two effects of quantum size and electron density in nanoparticle's conduction bands, and which one played a major role. The poisonous organic contaminants such as anilines, could induce the aggregation of AgNPs, were observed to affect effectively the luminol-H2O2-7 nm and 15 nm AgNPs CL systems and were detectable by use of a flow injection method with the enhanced or inhibited CL detection.

Qi, Yingying; Li, Baoxin; Xiu, Furong

2014-07-01

36

Effect of aggregated silver nanoparticles on luminol chemiluminescence system and its analytical application.  

PubMed

We found that after silver nanoparticles (AgNPs) aggregated, its catalytic activity on luminol CL reaction obviously changed, and the change characteristic was closely related to the sizes of AgNPs. UV-visible spectra, X-ray photoelectron spectra, zeta potential and transmission electron microscopy studies were carried out to investigate the CL effect mechanism. The different CL responses of aggregated AgNPs with different size were suggested to be due to the two effects of quantum size and electron density in nanoparticle's conduction bands, and which one played a major role. The poisonous organic contaminants such as anilines, could induce the aggregation of AgNPs, were observed to affect effectively the luminol-H2O2-7nm and 15nm AgNPs CL systems and were detectable by use of a flow injection method with the enhanced or inhibited CL detection. PMID:24662755

Qi, Yingying; Li, Baoxin; Xiu, Furong

2014-07-15

37

Investigation of the radical step in the chemiluminescent oxidation of luminol by potassium ferricyanide in the presence of hydrogen peroxide with the aid of magnetic modulation  

SciTech Connect

A method employing magnetic modulation of the rate of the chemical reaction was previously applied with success to the establishment of the chemiluminescent reaction involving the oxidation of luminol (LH/sub 2/) by potassium ferricyanide K/sub 3/Fe-(CN)/sub 6/ in an aqueous solution of sodium hydroxide (0.1 M NaOH). The purpose of the present work was to experimentally investigate the radical steps of the more complex chemiluminescent reaction involving the oxidation of LH/sub 2/ in the presence of H/sub 2/O/sub 2/. The action of the magnetic field on the reaction causes an increase in the intensity of the chemiluminescence. The dependence of the intensity of the chemiluminescence on the magnetic field strength has the form of a saturation curve. The hyperfine interaction of the electronic and nuclear spins of the recombining radicals create a possibility for the mixing of the singlet and triplet states of the radical pairs, which results in relative alteration of the populations of these states with the rate constant K/sub st/(H). An external magnetic field reduces this constant, causing an increase in the concentration of the LOH/sup 2 -/ radicals and, consequently, an increase in the output of light.

Tribel', M.M.; Morozov, A.K.; Frankevich, E.L.

1987-11-01

38

Study of Chemiluminescent Reactions of Metal Atoms.  

National Technical Information Service (NTIS)

Laser vaporization of thin metal films has been used as the metal atom beam source for the study of chemiluminescent oxidation reactions. Techniques have been developed and demonstrated to experimentally determine the chemiluminescent reaction stoichiomet...

J. F. Friichtenicht, S. P. Tang, B. G. Wicke

1977-01-01

39

Enhanced effect of aggregated gold nanoparticles on luminol chemiluminescence system and its analytical application.  

PubMed

Some organic compounds containing groups of OH, NH2, or SH, which could induce the aggregation of gold nanoparticles (AuNPs), were observed to enhance effectively the luminol-H2O2-2.6 nm AuNPs CL system. It was found that the aggregation of AuNPs was an important effect factor for the catalytic activity of AuNPs on luminol CL system. The aggregated AuNPs could effectively enhance luminol CL signal compared with the dispersed one. The enhanced effect was closely related to the sizes of AuNPs. Among the studied AuNPs with seven sizes, 2.6 nm AuNPs had the greatest enhancement effect on luminol CL system after its aggregation. The CL enhancement mechanism was investigated, and the marked enhancement of aggregated 2.6 nm AuNPs for luminol CL system was supposed to originate from the decrease of AuNPs' surface negative charge density compared to its dispersed state. For the luminol-H2O2-2.6 nm AuNPs CL system in the presence of organic compounds containing groups of OH, NH2, or SH, more than one factor played the role in influencing the CL intensity. It was found that the enhanced effect of aggregated 2.6 nm AuNPs induced by such organic compounds was much more significant than the inhibition effect of reducing groups of OH, NH2, or SH, which made it applicable for the determination of this kind of compounds. PMID:23602952

Qi, Yingying; Li, Baoxin

2013-07-01

40

A Study of Oxalic Ester Chemiluminescent Reactions.  

National Technical Information Service (NTIS)

The effects of a number of reaction variables and additives on the kinetics and quantum yields of oxalic ester chemiluminescent reactions were investigated. The experiments were successfully designed to distinguish variable effects on processes leading to...

M. M. Rauhut A. M. Semsel

1974-01-01

41

Determination of dissolved Fe(II) in seawater of the western North Pacific with luminol chemiluminescence method  

NASA Astrophysics Data System (ADS)

Determination of dissolved Fe(II) in seawater of the western North Pacific with luminol chemiluminescence method Hajime Obata, Akira Mase, Toshitaka Gamo (Atmosphere and Ocean Research Institute, University of Tokyo, Japan), Jun Nishioka (Institute of Low Temperature Science, Hokkaido University, Japan), Shigenobu Takeda (Faculty of Fisheries, Nagasaki University, Japan) Speciation of iron in the ocean is now important topics because the bioavailability of iron depends on its chemical form in seawater. However, marine biogeochemical process of Fe(II) has not been fully investigated. In this study, we determined Fe(II) in seawaters using the luminol chemiluminescence method after acidifying the samples to pH 6(Hansard and Landing, 2009). The same samples collected in the western North Pacific were analyzed by the flow chemiluminescence methods with acidification to pH 6 and without acidification. The results with both methods were almost identical. Time variation of Fe(II) in seawater after acidifying the samples to pH 6 were examined in the western North Pacific and the Bering Sea. Within 10 minutes, variations of Fe(II) were small in the open ocean waters, whereas Fe(II) concentrations increased rapidly in surface waters collected in the Bering Sea. The acidification method is not always applicable for seawater samples, especially in the marginal sea. Surface distributions of Fe(II) in the western subarctic North Pacific were investigated by using a continuous clean sampling system for surface waters. The Fe(II) concentrations ranged from <9 to 42 pM, which were lower than those in previous studies (Roy et al., 2008). The variation of Fe(II) probably reflects the photoreduction process of Fe(III), slow oxidation of Fe(II) and differences of Fe(II) concentrations among water masses. In this study, we also examined the oxidation process of Fe(II) in seawater of the western North Pacific and the Bering Sea at some temperatures. The oxidation rates were slower in the Bering Sea than those in the western North Pacific, implying that the oxidation rates were controlled not only by water temperature but also by organic compounds, such as humic substances.

Obata, H.; Mase, A.; Gamo, T.; Nishioka, J.; Takeda, S.

2010-12-01

42

Research and development of a luminol-carbon monoxide flow system  

NASA Technical Reports Server (NTRS)

Adaption of the luminol-carbon monoxide injection system to a flowing type system is reported. Analysis of actual wastewater samples was carried out and revealed that bacteria can be associated with particles greater than 10 microns in size in samples such as mixed liquor. Research into the luminol reactive oxidation state indicates that oxidized iron porphyrins, cytochrome-c in particular, produce more luminol chemiluminescence than the reduced form. Correlation exists between the extent of porphyrin oxidation and relative chemiluminescence. In addition, the porphyrin nucleus is apparently destroyed under the current chemiluminescent reaction conditions.

Thomas, R. R.

1977-01-01

43

CoFe2O4 nanoparticles as oxidase mimic-mediated chemiluminescence of aqueous luminol for sulfite in white wines.  

PubMed

Recently, the intrinsic enzyme-like activity of nanoparticles (NPs) has become a growing area of interest. However, the analytical applications of the NP-based enzyme mimetic are mainly concentrated on their peroxidase-like activity; no attempts have been made to investigate the analytical applications based on the oxidase mimic activities of NPs. For the first time, we report that CoFe(2)O(4) NPs were found to possess intrinsic oxidase-like activity and could catalyze luminol oxidation by dissolved oxygen to produce intensified chemiluminescence (CL). The effect of sulfite on CoFe(2)O(4) NP oxidase mimic-mediated CL of aqueous luminol was investigated. It is very interesting that when adding sulfite to the luminol-CoFe(2)O(4) system, the role of sulfite in the luminol-CoFe(2)O(4) NP-sulfite system depends on its concentration. At a relatively low concentration level, sulfite presents an inhibition effect on the luminol-CoFe(2)O(4) NP system. However, it does have an enhancement effect at a higher concentration level. Investigations on the effect of the solution pH and luminol and CoFe(2)O(4) NP concentrations on the kinetic characteristics of the studied CL system in the presence of trace sulfite suggested that the enhancement and inhibition of the luminol-CoFe(2)O(4) NP-sulfite CL system also depended on the solution pH. It seems that the concentrations of luminol and CoFe(2)O(4) NPs did not influence the CL pathway. The possible mechanism of the luminol-CoFe(2)O(4) NP-sulfite CL system was also discussed. On this basis, a flow injection chemiluminescence method was established for the determination of trace sulfite in this study. Under the optimal conditions, the proposed system could respond down to 2.0 × 10(-8) M sulfite. The method has been applied to the determination of trace sulfite in white wine samples with satisfactory results. The results given by the proposed method are in good agreement with those given by the standard titration method. PMID:23289402

Zhang, Xiaodan; He, Shaohui; Chen, Zhaohui; Huang, Yuming

2013-01-30

44

Alterations in luminol-enhanced chemiluminescence from nondiluted whole blood in the course of low-level laser therapy of angina pectoris patients  

NASA Astrophysics Data System (ADS)

Addition of Luminol to nondiluted blood of healthy donors results in a short and weak increase of chemiluminescence (CL) from it. Contrary to that in 25 cases of stable angina pectoris the intensity of CL from blood of patients sharply increased upon addition of luminol exceeding that form healthy donors' blood 10-100-fold. 24 hours after the 3D intravenous low-level treatment CL burst in patients' blood in the presence of Luminol was in general significantly lower than before the beginning of the treatment. After the 7th treatment the pattern of CL kinetics was in most cases similar to that of healthy donors' blood. However, after the 10th treatment intensity of Luminol-enhanced CL usually increased and for blood of some patients even exceeded its values obtained before the treatment. Some correlation CL from nondiluted blood with neutrophil activity studied by NTB-test and plasma viscosity of same blood was noted. Using highly sensitive single photon counters it is possible to reveal abnormal levels of CL from no more than 0.1-0.2 ml of blood within 3-5 min.

Voeikov, Vladimir L.; Novikov, Cyril N.; Siuch, Natalia I.

1997-05-01

45

Rapid determination of isoamyl nitrite in pharmaceutical preparations by flow injection analysis with on-line UV irradiation and luminol chemiluminescence detection.  

PubMed

Isoamyl nitrite is used as a therapeutic reagent for cardiac angina and as an antidote for cyanide poisoning, but it is abused because of its euphoric properties. Therefore, a method to determine isoamyl nitrite is required in many fields, including pharmaceutical and forensic studies. In this study, a simple, rapid and sensitive method for the determination of isoamyl nitrite was developed using a flow injection analysis system equipped with a chemiluminescence detector and on-line photoreactor. This method is based on on-line ultraviolet irradiation of isoamyl nitrite and subsequent luminol chemiluminescence detection without the addition of an oxidant. A linear standard curve was obtained up to 1.0 ?M of isoamyl nitrite with a detection limit (blank + 3SD) of 0.03 ?M. The method was successfully applied to determine isoamyl nitrite content in pharmaceutical preparations. PMID:23299968

Kishikawa, Naoya; Kondo, Naoko; Amponsaa-Karikari, Abena; Kodamatani, Hitoshi; Ohyama, Kaname; Nakashima, Kenichiro; Yamazaki, Shigeo; Kuroda, Naotaka

2014-02-01

46

Fast simultaneous determination of traces of Cu(II) and Co(II) in soils and sediments with the luminol/perborate chemiluminescent system.  

PubMed

A flow injection analysis method based on ion chromatography and luminol chemiluminescence detection was used for the simultaneous determination of copper (II) and cobalt (II) trace levels in soils and sediments following microwave-assisted acid digestion. Detection was based on chemiluminescence (CL) of the luminol-perborate system in an alkaline medium, which is catalyzed by both transition metals. The concentration and pH of the eluent (oxalic acid) was found to affect CL intensities and retention times, both of which were inversely proportional to the oxalic acid concentration. The calibration curves for both metal ions were linear and allowed a limit of detection of 0.003 ?g l(-1) for cobalt (II) and 0.014 ?g l(-1) for cooper (II) to be calculated. The proposed method was successfully used to determine both metal ions in certified reference materials of stream and river sediments and soil samples. Based on the results, the determination is free of interferences from the usual concomitant ions. PMID:22392616

Pulgarín, José A Murillo; Bermejo, Luisa F García; Durán, Armando Carrasquero

2013-01-01

47

Comparative study of ?-glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminol-enhanced chemiluminescence assay in common carp (Cyprinus carpio L.).  

PubMed

The respiratory burst is an important feature of the immune system. The increase in cellular oxygen uptake that marks the initiation of the respiratory burst is followed by the production of reactive oxygen species (ROS) such as superoxide anion and hydrogen peroxide which plays a role in the clearance of pathogens and tissue regeneration processes. Therefore, the respiratory burst and associated ROS constitute important indicators of fish health status. This paper compares two methods for quantitation of ROS produced during the respiratory burst in common carp: the widely used, single-point measurement based on the intracellular reduction of nitroblue tetrazolium (NBT) and a real-time luminol-enhanced assay based on the detection of native chemiluminescence. Both assays allowed for detection of dose-dependent changes in magnitude of the respiratory burst response induced by ?-glucans in head kidney cells of carp. However, whereas the NBT assay was shown to detect the production of only superoxide anions, the real-time luminol-enhanced assay could detect the production of both superoxide anions and hydrogen peroxide. Only the chemiluminescence assay could reliably record the production of ROS on a real-time scale at frequent and continual time intervals for time course experiments, providing more detailed information on the respiratory burst response. The real-time chemiluminescence assay was used to measure respiratory burst activity in macrophage and neutrophilic granulocyte-enriched head kidney cell fractions and total head kidney cell suspensions and proved to be a fast, reliable, automated multiwell microplate assay to quantitate fish health status modulated by ?-glucans. PMID:23454430

Vera-Jimenez, N I; Pietretti, D; Wiegertjes, G F; Nielsen, M E

2013-05-01

48

Time-resolved chemiluminescence study of the TiO2 photocatalytic reaction and its induced active oxygen species.  

PubMed

The time-resolved chemiluminescence (CL) method has been applied to study the TiO(2) photocatalytic reaction on a micros-ms timescale. The experimental set-up for time-resolved CL was improved for confirmation of the unique luminol CL induced by the photocatalytic reaction. The third harmonic light (355 nm) from an Nd:YAG laser was used for the light source of the TiO(2) photocatalytic reaction. Luminol CL induced by this reaction was detected by a photomultiplier tube (PMT) and a preamplifier was used for amplifying the CL signal. Experimental conditions affecting the photocatalytically induced CL were discussed in detail. The involvement of active oxygen species such as .OH, O(2) (.-) and H(2)O(2) in the CL was examined by adding their scavengers. It is concluded that .OH was greatly involved in the CL on a micros-ms timescale, especially in time periods <100 micros after illumination of the pulse laser. On the other hand, CL generated by O(2) (.-) began to increase after 100 micros and became dominant after 2.5 ms. A small part of the CL might be generated by H(2)O(2) on the whole micros-ms timescale. A CL reaction mechanism related with .OH and dissolved oxygen was proposed to explain the photocatalytically induced luminol CL on a micros-ms timescale, especially in periods <100 micros. PMID:17089368

Min, Lingyue; Wu, Xing-Zheng; Tetsuya, Shimada; Inoue, Haruo

2007-01-01

49

Flow-injection chemiluminescence determination of chlorinated isocyanuric acids  

Microsoft Academic Search

A rapid and sensitive flow-injection chemiluminescence method is described for the determination of dichloro- and trichloroisocyanuric acids based on the chemiluminescence produced during their reaction with luminol in alkaline medium. The effects of analytical and flow-injection variables on these chemiluminescence systems and determination of both oxidants are discussed. The optimized method yielded 3? detection limits of 8×10-8 and 5×10-8 mol

Afsaneh Safavi; Mohammad Ali Karimi

2003-01-01

50

Flow-injection chemiluminescence determination of chlorinated isocyanuric acids  

Microsoft Academic Search

A rapid and sensitive flow-injection chemiluminescence method is described for the determination of dichloro- and trichloroisocyanuric acids based on the chemiluminescence produced during their reaction with luminol in alkaline medium. The effects of analytical and flow-injection variables on these chemiluminescence systems and determination of both oxidants are discussed. The optimized method yielded 3† detection limits of 8᎒-8 and 5᎒-8 mol

Afsaneh Safavi; Mohammad Ali Karimi

2003-01-01

51

Development and optimization of an analytical method for the determination of Sudan dyes in hot chilli pepper by high-performance liquid chromatography with on-line electrogenerated BrO- -luminol chemiluminescence detection.  

PubMed

The determination of four Sudan dyes by means of high-performance liquid chromatography (HPLC) with chemiluminescence (CL) detection was proposed. The method was based on the enhancement effect of Sudan dyes on the chemiluminescence reaction between luminol and BrO-, which was on-line electrogenerated by constant current electrolysis. The separation was carried out on Nucleosil RP-C18 column (250 mm x 4.6 mm i.d., 5 microm, pore size, 100 A) at 35 degrees C. The mobile phase consisted of a V (methanol): V (0.2% aqueous formic acid) = 90:10 solution. At a flow-rate of 1.0 ml min(-1), the total run time was 25 min. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. For the four Sudan dyes, the limits of detection (LOD) at a signal-to-noise of 3 ranged from 4 to 8 microg kg(-1) and the limits of quantification (LOQ) at a signal-to-noise of 10 ranged from 13 to 27 microg kg(-1). The relative standard deviations (RSD) of intra-and inter-day precision were below 4.4%. The average recoveries for all four Sudan dyes (spiked at the levels of 1.0 and 1.5 mg kg(-1)) in chilli tomato sauce and hot chilli pepper ranged from 94% to 105%, and the relative standard deviations of the quantitative results were from 2.5 to 4.2%. The proposed method had been successfully applied to the determination of four Sudan dyes in hot chilli products. PMID:16919285

Zhang, Yantu; Zhang, Zhujun; Sun, Yonghua

2006-09-29

52

Highly sensitive method for determination of N-nitrosamines using high-performance liquid chromatography with online UV irradiation and luminol chemiluminescence detection.  

PubMed

A new method for the measurement of N-nitrosamines in part-per-trillion concentrations from water samples without preconcentration steps has been developed. This method is based on online UV irradiation after high-performance liquid chromatographic separation and subsequent luminol chemiluminescence detection without addition of an oxidant. It was confirmed that N-nitrosamines in basic aqueous solution were transformed to peroxynitrite by UV irradiation. The detection limits for this method were 1.5 ng/L, 2.9 ng/L, 3.0 ng/L, and 2.7 ng/L for N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine, respectively, at a signal-to-noise ratio of 3. The calibration graphs were linear in the range of 5-1000 ng/L for these N-nitrosamines. This method was used for the determination of N-nitrosamines in tap water, river water, and industrial plant effluent samples. The recoveries of N-nitrosodimethylamine, N-nitrosomorpholine, N-nitrosomethylethylamine, and N-nitrosopyrrolidine present in tap water sample at a concentration of 10 ng/L (mean+/-standard deviation, n=4) were (94.8+/-2.7)%, (102.0+/-6.9)%, (99.3+/-3.9)%, and (102.8+/-2.5)%, respectively. These results indicate that our proposed method can be applied satisfactorily to the determination of N-nitrosamines in water samples. PMID:19062029

Kodamatani, Hitoshi; Yamazaki, Shigeo; Saito, Keiitsu; Amponsaa-Karikari, Abena; Kishikawa, Naoya; Kuroda, Naotaka; Tomiyasu, Takashi; Komatsu, Yu

2009-01-01

53

An optimized, sensitive and stable reduced graphene oxide–gold nanoparticle-luminol-H2O2 chemiluminescence system and its potential analytical application  

NASA Astrophysics Data System (ADS)

The chemiluminescence (CL) performance of luminol is improved using reduced graphene oxide/gold nanoparticle (rGO–AuNP) nano-composites as catalyst. To prepare this catalyst, we propose a linker free, one-step method to in-situ synthesize rGO–AuNP nano-composites. Various measurements are utilized to characterize the resulting rGO–AuNP samples, and it is revealed that rGO could improve the stability and conductivity. Furthermore, we investigate the CL signals of luminal catalyzed by rGO–AuNP. Afterwards, the size effect of particle and the assisted enhancement effect of rGO are studied and discussed in detail. Based on the discussion, an optimal, sensitive and stable rGO–AuNP-luminon–H2O2 CL system is proposed. Finally, we utilize the system as a sensor to detect hydrogen peroxide and organic compounds containing amino, hydroxyl, or thiol groups. The CL system might provide a more attractive platform for various analytical devices with CL detection in the field of biosensors, bioassays, and immunosensors.

Wang, Wen-Shuo; He, Da-Wei; Wang, Ji-Hong; Duan, Jia-Hua; Peng, Hong-Shang; Wu, Hong-Peng; Fu, Ming; Wang, Yong-Sheng; Zhang, Xi-Qing

2014-04-01

54

Luminol and diazoluminomelanin as indicators of HL-60 cell differentiation.  

PubMed

This paper describes use of a novel substituted melanin which is useful in detection of differentiating leukemia cells and their membranes. Comparisons of luminol-(5-amino-2,3-dihydro-1,4-phthalazinedione) and diazoluminomelanin (DALM)-mediated chemiluminescence (CL) were made with various types of differentiated and undifferentiated HL-60 whole cells, cell lysates, and membrane fractions. Luminol had a greater CL response than DALM with HL-60 promyelocytic stem cells and differentiated macrophage-like or neutrophil-like whole cell and cell lysate preparations. However, DALM showed markedly greater CL than luminol for membrane fractions derived from each cell type. The greatest luminol-dependent CL was observed for cell types high in myeloperoxidase (MPO). The greatest DALM-mediated CL was seen with cell types that are high in MPO or strong producers of superoxide (O2-) anions. In some cases, significant differences in CL could also be distinguished on the basis of inducing agent used [i.e. dimethylsulfoxide, all-trans retinoic acid or 12-o-tetradecanoylphorbol-13-acetate]. Both luminol- and DALM-dependent CL were strongly inhibited by preincubation of cellular preparations with 3-amino-L-tyrosine (a component of DALM). Taken together, these data suggest that the reaction mechanism of luminol favors interaction with cytoplasmic MPO whereas that of DALM favors membrane interactions. Thus, both reagents may be of use in assays to detect differentiating leukocytes or their cellular components. PMID:8407717

Bruno, J G; Kiel, J L

1993-09-01

55

High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection.  

PubMed

An HPLC method was developed for the determination of citric, lactic, malic, oxalic and tartaric acids by chemiluminescent detection following online irradiation with visible light. The organic acids were irradiated with visible light in the presence of Fe3+ and UO2(2+) to generate Fe2+, which was determined by measuring the chemiluminescence intensity in a luminol system in the absence of added oxidant. Factors affecting the photochemical and chemiluminescence reactions were optimised so that their contribution to the total band-broadening was negligible. The chromatographic separation was performed on a C18 column under isocratic reversed-phase conditions using 0.005 M H2SO4 mobile phase. The optimised method was validated with respect to linearity, precision, limits of detection and quantification, accuracy specificity and robustness. The applicability of the assay was demonstrated by analysing these compounds in real samples such as milk, fruit juices, soft drinks, wine and beer. PMID:14763733

Pérez-Ruiz, Tomás; Martínez-Lozano, Carmen; Tomás, Virginia; Martín, Jesús

2004-02-13

56

Surface-mediated chemiluminescent reaction of O and NO  

NASA Astrophysics Data System (ADS)

Deep-red chemiluminescence occurs when beams of O and NO react on metal surfaces. Of the metals studied (Ni, Co, Pt; Kovar, Invar, and Monel), nickel was the most effective at promoting the chemiluminescent reaction. The rate depends inversely on the surface temperature. The reaction rate appears to be controlled by the flux of NO to the surface, and does not depend strongly on the NO beam temperature. These behaviors indicate that the chemiluminescence arises not from a gas-phase reaction near the surface, but from a surface-mediated reaction of O and NO to produce electronically excited gas-phase NO 2, most probably of the Langmuir—Hinshelwood variety.

Arnold, Graham S.; Coleman, Dianne J.

1991-02-01

57

Chemiluminescent Reaction of Tellurium with Fluorine.  

National Technical Information Service (NTIS)

Tellurium vapor reacts with gaseous fluorine to produce a chemiluminescent flame. The emission in the visible and near infrared part of the spectrum of the flame has been previously attributed to the A2 psi - X 2 psi - X system of TeF. Excited TeF produce...

H. W. Moody J. A. Menapace C. J. Dymek M. L. Scott

1982-01-01

58

Mechanism of Chemiluminescent Combination Reactions Involving Oxygen Atoms  

Microsoft Academic Search

The intensities I_c and I_a respectively of chemiluminescent emission by CO_2 in the O+CO reaction and by NO_2 in the O+NO reaction have been measured from 200 to 300 ^circK in a fast flow system. Both I_a and I_c were found to obey an expression of the type I=I_0[O] [XO], where I_0 was independent of total pressure over a similar

M. A. A. Clyne; B. A. Thrush

1962-01-01

59

Effect of colloidal ?-cyclodextrins-Fe3 O4 magnetic nanoparticles on the chemiluminescence enhancement of luminol-Ag(III) complex for rapid and sensitive determination of cysteine in human serum.  

PubMed

Colloidals solution of Fe3 O4 magnetic nanoparticles (MNPs), capped with ?-cyclodextrins (?-CD) as inclusion complexes, were found to enhance the chemiluminescence (CL) intensity of the luminol-diperiodatoargentate(III) (DPA) system. On injection of cysteine into the luminol-DPA-?-CD-Fe3 O4 MNPs inclusion complexes system, the CL intensity is strongly enhanced. The enhanced CL signal is ascribed to the catalytic effect of Fe3 O4 MNPs capped with ?-CD, which is assumed to stabilize the CL intermediate. Based on these findings, a rapid and sensitive assay was developed for the determination of cysteine in human serum. The effects of analytical variables on the CL signal were studied and optimized. Under the optimum conditions, the CL intensity was directly proportional to the concentration of cysteine in the range 8.0?×?10(-9) -1.0?×?10(-6) ?mol/L. The detection limit was 2.8?×?10(-9) ?mol/L (3 S(b) /m) and the relative standard deviation (RSD) for 10 replicate determinations of 1.0?×?10(-7) ?mol/L cysteine was 3.5%. The proposed method was applied to the sensitive determination of cysteine in human serum samples, and compared with the Ellman method with satisfactory results. PMID:22025250

Rezaei, B; Ensafi, A A; Zarei, L; Kameli, P

2012-01-01

60

Flow-injection chemiluminescence determination of chlorinated isocyanuric acids.  

PubMed

A rapid and sensitive flow-injection chemiluminescence method is described for the determination of dichloro- and trichloroisocyanuric acids based on the chemiluminescence produced during their reaction with luminol in alkaline medium. The effects of analytical and flow-injection variables on these chemiluminescence systems and determination of both oxidants are discussed. The optimized method yielded 3sigma detection limits of 8x10(-8) and 5x10(-8) mol L(-1) for the sodium dichloroisocyanurate and trichloroisocyanuric acid, respectively. The optimum conditions were found to be as follows: NaOH, 1x10(-1) mol L(-1); luminol, 5x10(-3) mol L(-1); KI, 2x10(-3) mol L(-1) and flow rate, 3.5 mL min(-1). PMID:12589508

Safavi, Afsaneh; Karimi, Mohammad Ali

2003-02-01

61

Enhanced chemiluminescent method for the detection of DNA dot-hybridization assays.  

PubMed

A simple enhanced chemiluminescent procedure for the quantitation of DNA hybridization to dot blots is described. The method utilizes DNA probes labeled with biotin, which are detected using a biotinylated streptavidin-horseradish peroxidase complex. The peroxidase enzyme then takes part in an enhanced chemiluminescent reaction with luminol, peroxide, and an enhancer. The method can be used to give quantitative results using a photomultiplier tube or qualitative results by recording the light emission on instant photographic film. PMID:4091282

Matthews, J A; Batki, A; Hynds, C; Kricka, L J

1985-11-15

62

Micro-plate chemiluminescence enzyme immunoassay for aflatoxin B1 in agricultural products  

Microsoft Academic Search

In this work, a micro-plate chemiluminescence enzyme immunoassay by antibody-coated for the determination of aflatoxin B1 (AFB1) in agricultural products has been established. Aflatoxin B1 antibody (AFB1-Ab) was adsorbed physically on polystyrene micro-plate hole as solid phase antibody, which took place immunity-reaction between antigen and antibody with AFB1 standard solution or samples by direct competition. Luminol–hydrogen peroxide chemiluminescence system catalyzed

Luqiu Fang; Hui Chen; Xitang Ying; Jin-Ming Lin

2011-01-01

63

Chemiluminescence measurements as an identification method for gamma-irradiated foodstuffs  

NASA Astrophysics Data System (ADS)

Samples of 19 different spices, milk powder, whole onions and frozen chicken were exposed to a Co-60 source with radiation doses up to 10 4 Gy. The subsequent reaction of the irradiated foodstuffs in a luminol solution resulted in light emission (chemiluminescence). This effect can be used as an indicator of radiation treatment.

Bögl, W.; Heide, L.

64

Graphene-based chemiluminescence resonance energy transfer for homogeneous immunoassay.  

PubMed

We report on chemiluminescence resonance energy transfer (CRET) between graphene nanosheets and chemiluminescent donors. In contrast to fluorescence resonance energy transfer, CRET occurs via nonradiative dipole-dipole transfer of energy from a chemiluminescent donor to a suitable acceptor molecule without an external excitation source. We designed a graphene-based CRET platform for homogeneous immunoassay of C-reactive protein (CRP), a key marker for human inflammation and cardiovascular diseases, using a luminol/hydrogen peroxide chemiluminescence (CL) reaction catalyzed by horseradish peroxidase. According to our results, anti-CRP antibody conjugated to graphene nanosheets enabled the capture of CRP at the concentration above 1.6 ng mL(-1). In the CRET platform, graphene played a key role as an energy acceptor, which was more efficient than graphene oxide, while luminol served as a donor to graphene, triggering the CRET phenomenon between luminol and graphene. The graphene-based CRET platform was successfully applied to the detection of CRP in human serum samples in the range observed during acute inflammatory stress. PMID:22417160

Lee, Joon Seok; Joung, Hyou-Arm; Kim, Min-Gon; Park, Chan Beum

2012-04-24

65

CoFe2O4 magnetic nanoparticles as a peroxidase mimic mediated chemiluminescence for hydrogen peroxide and glucose.  

PubMed

This communication presents a new peroxidase mimic of CoFe(2)O(4) nanoparticles evaluated by the luminol-based chemiluminescent (CL) reaction. This offers a new method for evaluation and screening of the nanoparticles-based enzyme mimetics. PMID:21892508

Shi, Wenbing; Zhang, Xiaodan; He, Shaohui; Huang, Yuming

2011-10-14

66

Determination of phenolic compounds and their antioxidant activity in Erigeron acris L. extracts and pharmaceutical formulation by flow injection analysis with inhibited chemiluminescent detection  

Microsoft Academic Search

It was found that the chemiluminescence (CL) produced from the reaction of luminol with iodine in the alkaline medium was strongly inhibited by plant phenolic compounds. Based on this finding, a new flow injection CL method was developed for the determination of caffeic acid and 6?-caffeoylerigeroside. The latter compound was isolated for the first time from Erigeron acris L. herb.

Edyta Nalewajko-Sieliwoniuk; Jolanta Nazaruk; Ewelina Antypiuk; Anatol Koj?o

2008-01-01

67

Development of a chemiluminescent and bioluminescent system for the detection of bacteria in wastewater effluent  

NASA Technical Reports Server (NTRS)

Automated chemiluminescent and bioluminescent sensors were developed for continuous monitoring of microbial levels in wastewater effluent. Development of the chemiluminescent system included optimization of reagent concentrations as well as two new techniques which will allow for increased sensitivity and specificity. The optimal reagent concentrations are 0.0025 M luminol and 0.0125 M sodium perborate in 0.75N sodium hydroxide before addition of sample. The methods developed to increase specificity include (1) extraction of porphyrins from bacteria collected in a filter using 0.1N NaOH - 50 percent Ethanol, and (2) use of the specific reaction rate characteristics for the different luminol catalysts. Since reaction times are different for each catalyst, the reaction can be made specific for bacteria by measuring only the light emission from the particular reaction time zone specific for bacteria. Developments of the bioluminescent firefly luciferase system were in the area of flow system design.

Thomas, R. R.

1975-01-01

68

On the Luminescence of Luminol V. Mechanism of the Catalytic Action of Iron Complexes.  

National Technical Information Service (NTIS)

A study was made of the effect of temperature on the chemiluminescence of luminol in the presence of the following catalysts: hemin chloride, salicylaldehyde ethylenene diamine-ferric chloride, hemoglobin, and potassium ferrocyanide. The first two of thes...

K. Weber

1965-01-01

69

Alloxan-induced luminol luminescence as a tool for investigating mechanisms of radical-mediated diabetogenicity.  

PubMed

Chemiluminescence of luminol in a cell-free system was used to investigate the mechanism of alloxan-dependent free-radical generation. In the presence of alloxan and reduced glutathione (GSH), luminescence was greatly stimulated by FeSO4. Replacing GSH by oxidized glutathione or NAD(P)(H), or replacing FeSO4 by CuSO4, ZNSO4 or FeCl3, did not yield chemiluminescence. The chemiluminescence of a mixture of alloxan. GSH, FeSO4 and luminol was inhibited by catalase, superoxide dismutase, scavengers of hydroxyl radicals (sodium benzoate, n-butanol, D-mannitol, dimethyl sulphoxide) or metal-ion chelators (EDTA, diethylenetriaminepenta-acetic acid, diethyldithiocarbamate. desferroxamine), D-glucose, L-glucose, D-mannose, D-fructose, 3-O-methyl-D-glucose, NAD+, NADH, NADP+ or NADPH, but not by urea or enzymically inactive superoxide dismutase. The results support the hypothesis that the diabetogenic action of alloxan is mediated by hydroxyl radicals generated in an iron-catalysed reaction. Protection against alloxan in vivo depends both on the chemical reactivity of protector with radicals or radical-generating systems and on the stereospecific requirement of some strategic site in the B-cell. PMID:7342976

Grankvist, K

1981-12-15

70

Cu + ion core conservation from reactant to product in the Cu+F 2 chemiluminescent reaction  

NASA Astrophysics Data System (ADS)

Electronic chemiluminescence from the reaction of selected ground state ( 2S 1/2) or metastable ( 2D 5/2, 2D 3/2) copper atoms with fluorine has been studied using a hollow cathode-flowing afterglow reactor. The observed signal related to the Cu( 2S) and Cu*( 2D) atom densities, indicate that the chemiluminescence cross-section for Cu*( 2D) atoms is about 10 4 times larger than for Cu( 2S) atoms. This strong propensity is explained in terms of a direct reaction, initiated by a harpooning process, during which the Cu + ion core of the reactant (3d 10 for Cu( 2S) and 3d 94s for Cu*( 2D)) is conserved in the products (ionic structure Cu +(3d 10)F - for the CuF ground state and Cu +(3d 94s)F - for the CuF*(a, A, B, C, D) chemiluminescent states).

Baltayan, P.; Hartmann, F.; Hikmet, L.; Pebay-Peyroula, J.-C.; Sadeghi, N.

1989-08-01

71

Te2 Chemiluminescence from Alkali Atom-TeCl4 Reactions.  

National Technical Information Service (NTIS)

The gas phase reaction between alkali atoms and TeCl4 produces chemiluminescence from the A state of Te2. The observed transitions are from V' = 0 - 5 of the A state to V = 3 - 20 of the X state. (Author)

K. K. Lin L. C. Balling J. J. Wright

1985-01-01

72

Development of a Chemiluminescence Method for the Simultaneous Determination of Ascorbic and Tartaric Acids Based Upon Their Reaction with Cerium(IV) in the Presence of Rutheniumtrisdipyridine  

Microsoft Academic Search

A time-resolved chemiluminescent method for the determination of ascorbic and tartaric acid has been developed. The method is based on the differential rate of the chemiluminescent reaction of Ru(bipy)3 , ascorbic and tartaric acid with Ce(IV). The ascorbic acid system gives the highest chemiluminescence intensity at 2s, whereas the tartaric acid system gives its most intense chemiluminescence emission at 40s.

Zhike He; Xiaoyan Li; Hui Meng; Shaofang Lu; Gongwu Song; Liangjie Yuan; Yune Zeng

1998-01-01

73

Luminol and diazoluminomelanin as indicators of HL60 cell differentiation  

Microsoft Academic Search

Summary  This paper describes use of a novel substituted melanin which is useful in detection of differentiating leukemia cells and\\u000a their membranes. Comparisons of luminol-(5-amino-2,3-dihydro-1,4-phthalazinedione) and diazoluminomelanin (DALM)-mediated\\u000a chemiluminescence (CL) were made with various types of differentiated and undifferentiated HL-60 whole cells, cell lysates,\\u000a and membrane fractions. Luminol had a greater CL response than DALM with HL-60 promyelocytic stem cells and

John G. Bruno; Johnathan L. Kiel

1993-01-01

74

Chemiluminescent logic gates based on functionalized gold nanoparticles/graphene oxide nanocomposites.  

PubMed

Label-free logic gates (AND, OR, and INHIBIT) based on chemiluminescence (CL) as new optical readout signal have been developed by taking advantage of the unique CL activity of luminol- and lucigenin-functionalized gold nanoparticles/graphene oxide (luminol-lucigenin/AuNPs/GO) nanocomposites. It was found that Fe(2+) ions could induce the CL emission of luminol-lucigenin/AuNPs/GO nanocomposites in alkaline solution. On this basis, by using Fe(2+) ions and NaOH as the inputs and the CL signal as the output, an AND logic gate was fabricated. When the initial reaction system contained luminol-lucigenin/AuNPs/GO nanocomposites and NaOH, either Fe(2+) ions or Ag(+) ions could react with the luminol-lucigenin/AuNPs/GO nanocomposites to produce a strong CL emission. This result was used to design an OR logic gate using Fe(2+) ions and Ag(+) ions as the inputs and CL signal as the output. Moreover, two INHIBIT logic gates for Fe(2+) and Ag(+) were also developed using by NaClO and L-cysteine as their CL inhibitors, respectively. Furthermore, the proposed logic gates were successfully used to detect Fe(2+), Ag(+), and L-cysteine, respectively. The developed logic gates may find future applications in sensing, clinical diagnostics, and environmental monitoring. PMID:23963613

He, Yi; Cui, Hua

2013-09-27

75

Synergism and chemiluminescence of cerium ions and ruthenium complexes in the belousov-zhabotinskii reaction  

SciTech Connect

This paper studies chemiluminescence (CL) in the system BrO/sup -//sub 3/-CH/sub 2/ (COOH)/sub 2/ -Ce/sup 3 +/,4+-RuPbipy)/sub 3/ /SUP 2+,/ /sub 3/. The tests were carried out in a CL/sup 3/ unit that included a light-tight chamber, a photoelectron multiplier (FEU-97), a VS-22 high voltage power pack, and an EPPV-60M recording potentiometer. The synergism in chemiluminescence at low concentrations of ruthenium complex does not appear in the oscillation parameters. The periodic CL of this two-catalyst system may be a convenient chemical model for the study of combined chemical reactions in more complicated biochemiluminescent processes, such as that by which the firefly flashes in the dark.

Karavaev, A.D.; Kazakov, V.P.; Tolstikov, G.A.

1986-04-01

76

Two techniques for eliminating luminol interference material and flow system configurations for luminol and firefly luciferase systems  

NASA Technical Reports Server (NTRS)

Two methods for eliminating luminol interference materials are described. One method eliminates interference from organic material by pre-reacting a sample with dilute hydrogen peroxide. The reaction rate resolution method for eliminating inorganic forms of interference is also described. The combination of the two methods makes the luminol system more specific for bacteria. Flow system designs for both the firefly luciferase and luminol bacteria detection systems are described. The firefly luciferase flow system incorporating nitric acid extraction and optimal dilutions has a functional sensitivity of 3 x 100,000 E. coli/ml. The luminol flow system incorporates the hydrogen peroxide pretreatment and the reaction rate resolution techniques for eliminating interference. The functional sensitivity of the luminol flow system is 1 x 10,000 E. coli/ml.

Thomas, R. R.

1976-01-01

77

Determination of terbutaline sulfate by capillary electrophoresis with chemiluminescence detection.  

PubMed

A novel capillary electrophoresis (CE) with chemiluminescence (CL) detection method for determination of terbutaline sulfate has been developed. This method is based on the chemiluminescence reaction of potassium ferricyanide with luminol in sodium hydroxide medium sensitized by terbutaline sulfate. With the peak height as a quantitative parameter applying optimum working conditions, terbutaline sulfate is determined over the range of 7.0 x 10(-8) to 3.6 x 10(-6)M with a detection limit of 3.0 x 10(-8)M. The relative standard deviation (RSD) was 4.6% for 6.0 x 10(-7)M terbutaline sulfate (n=11). The proposed method has been applied to determination of terbutaline sulfate in commercial terbutaline sulfate drug and spiked in human urine with satisfactory results. PMID:19109076

Li, Shuting; Wang, Janshi; Zhao, Shulin

2009-01-15

78

Determination of branched-chain L-amino acids by flow-injection analysis with co-immobilized leucine dehydrogenase\\/NADH oxidase and chemiluminescence detection  

Microsoft Academic Search

A method for the determination of branched-chain L-amino acids in plasma is described. Leucine dehydrogenase and NADH oxidase were co-immobilized on aminated poly(vinyl alcohol) beads and incorporated in a flow-injection system with chemiluminescence detection. 20 ?1 sample solution was injected into the system. The hydrogen peroxide produced was detected chemiluminometrically via a luminol-hexacyanoferrate(III) reaction. The calibration graph was linear for

Nobutoshi Kiba; Akira Kato; Motohisa Furusawa

1995-01-01

79

Chemiluminescence analysis  

SciTech Connect

Chemiluminescence (CL) is observed when light is emitted from a chemical reaction. If the reaction occurs in a living system or is derived from one, the process is called bioluminescence (BL). Many articles describe clinical, biological, and environmental applications using both gas- and solution-phase CL reactions. Although the lack of commercially available instrumentation, reagents, and methodology has somewhat restricted the widespread use of CL, certain applications are common: the measurement of total microbial cell counts using the firefly reaction and the determination of oxides of nitrogen with a gas-phase chemiluminescent reaction involving ozone. This REPORT will discuss only a few of the most recent developments in CL using reactions in solution.

Grayeski, M.L.

1987-11-01

80

Chemiluminescence detection of peroxynitrite with flow injection  

NASA Astrophysics Data System (ADS)

Peroxynitrite is an important derivative made by nitric oxide in vivo. It can make damages in many kinds of tissue and cells. Its research value in heart disease and cancer is a very high. A sensitive, specific method for analysis of peroxynitrite is described. In this method, chemiluminescence reaction between perodynitrite and luminol was used to detect with flow injection system. The assay has a detection limit of 2 by 10-8 mol L-1, and linear range of 5 by 10-8 mol L-1 to 5 by 10-5 mol L-1. The application o f flow injection system offers the possibility to establish biosensor for real-time detection of perodynitrite.

Kang, Dai; Evmiridis, Nick P.; Vlessidis, Athanasios; Zhou, Yikai

2001-09-01

81

Determination of soluble antigens of Clostridium botulinum A by chemiluminescent--immunosorbent assay (CLISA).  

PubMed

The chemiluminescent--immunosorbent assay (CLISA) was adopted for Cl. botulinum A soluble toxic antigens determination. Luminol (ABEI) labelled botulinum antitoxin globulins showed a strongly positive specific immunochemiluminescent reactions with the native botulinum toxin preparations coupled (adsorbed) on polystyrene balls. The sensitivity of the reaction reached 20 DLM/ml (5,000 light impulses per 40 sec) in comparison with 2 X 10(6) DLM (57,000 impulses), control preparations (1,500 impulses) and the background (150-300 impulses). The results give a perspective further investigations with the CLISA for rapid indication of Cl. botulinum toxic antigens. PMID:2431667

Ligieza, J; Michalik, M; Reiss, J; Grzybowski, J

1986-01-01

82

Crossed-Beam Chemiluminescence: The Alkaline Earth Rearrangement Reaction M + S2Cl2 yields S2 + MCl2.  

National Technical Information Service (NTIS)

The reaction of the heavier Group IIA metal atoms Ba, Sr, and Ca with S2Cl2 under crossed-beam conditions produces a chemiluminescent spectrum in which the S2 B 3 sigma(-) sub u - X 3 Sigma(-) sub g emission bands are the most prominent feature. In additi...

F. Engelke R. N. Zare

1976-01-01

83

Method and apparatus for eliminating luminol interference material  

NASA Technical Reports Server (NTRS)

A method and apparatus for removing porphyrins from a fluid sample which are unrelated to the number of bacteria present in the sample and prior to combining the sample with luminol reagent to produce a light reaction is disclosed. The method involves a pre-incubation of the sample with a dilute concentration of hydrogen peroxide which inactivates the interfering soluble porphyrins. Further, by delaying taking a light measurement for a predetermined time period after combining the hydrogen peroxide-treated water sample with a luminol reagent, the luminescence produced by the reaction of the luminol reagent with ions present in the solution, being short lived, will have died out so that only porphyrins within the bacteria which have been released by rupturing the cells with the sodium hydroxide in the luminol reagent, will be measured. The measurement thus obtained can then be related to the concentration of live and dead bacteria in the fluid sample.

Jeffers, E. L.; Thomas, R. R. (inventors)

1979-01-01

84

Chemiluminescence and electrochemiluminescence detection of controlled drugs.  

PubMed

We review the determination of various controlled drugs (opioids, tranquilizers, stimulants, and hallucinogens) using flow-analysis methodologies (flow injection analysis, high performance liquid chromatography, capillary electrophoresis, and microfluidic devices) with chemiluminescence and electrochemiluminescence reagents such as luminol, diaryloxalates, tris(2,2'-bipyridine)ruthenium(II), permanganate, manganese(IV), and sulfite, for industrial, clinical, pharmaceutical, and forensic science applications. PMID:21154734

Adcock, Jacqui L; Barrow, Colin J; Barnett, Neil W; Conlan, Xavier A; Hogan, Conor F; Francis, Paul S

2011-03-01

85

Electrogenerated ferrate(VI) for CE-chemiluminescence detection to determine benzenediol isomers.  

PubMed

Based on the quenched chemiluminescence intensity of the luminol reaction sensitized by ferrate(VI) in alkaline medium, a novel CE with on-line inhibited method for the simultaneous analysis of benzenediol isomers was developed. The ferrate(VI) solution was freshly prepared by electrochemical technique before electrophoresis. The parameters influencing detection and separation were carefully investigated. Baseline separation of benzenediols including catechol, resorcinol, and hydroquinone was achieved in less than 8 min with 5.0 mmol/L sodium tetraborate and 2.0 mmol/L luminol at an applied voltage of 18 kV. The LODs (S/N=3) for catechol, resorcinol and hydroquinone were determined to be 8.5 x 10(-9), 8.0 x 10(-7) and 6.5 x 10(-9) mol/L, respectively. Finally, the proposed method was applied for phenolic compounds in hair dye. PMID:20127910

Li, Fang; Hu, Yonggang; Zhang, Huijing; Zhang, Jie

2010-03-01

86

Comparison of Uric Acid Quantity with Different Food in Human Urine by Flow Injection Chemiluminescence Analysis  

PubMed Central

Based on the inhibitory effect of uric acid (UA) on luminol-Co2+ chemiluminescence (CL) system, a sensitive method for the determination of UA at nanomolar level by flow injection (FI) CL was proposed. The proposed method was successfully applied to real-time monitoring of UA excretion in human 24?h urine with different food intake, showing that meats, vegetables, and porridge intake caused differential UA excretions of 879, 798, and 742?mg, respectively. It was also found that UA concentrations in urine under the three kinds of food intake simultaneously reached maximum at 2?h after meals with the values of 417, 318, and 288??g?mL?1, respectively. The UA concentration in human serum was also determined by this approach, and the possible mechanism of luminol-Co2+-UA?CL reaction was discussed in detail.

Wang, Jiajia; Tan, Xijuan; Song, Zhenghua

2013-01-01

87

A competitive immunoassay for sensitive detection of small molecules chloramphenicol based on luminol functionalized silver nanoprobe.  

PubMed

Chloramphenicol (CHL) as a broad-spectrum antibiotic has a broad action spectrum against Gram-positive and Gram-negative bacteria, as well as anaerobes. The use of CHL is strictly restricted in poultry because of its toxic effect. However, CHL is still illegally used in animal farming because of its accessibility and low cost. Therefore, sensitive methods are highly desired for the determination of CHL in foodstuffs. The immunoassays based on labeling as an important tool have been reported for the detection of CHL residues in food-producing animals. However, most of the labeling procedures require multi-step reactions and purifications and thus they are complicated and time-consuming. Recently, in our previous work, luminol functionalized silver nanoparticles have been successfully synthesized, which exhibits higher CL efficiency than luminol functionalized gold nanoparticles. In this work, the new luminol functionalized silver nanoparticles have been used for the labeling of small molecules CHL for the first time and a competitive chemiluminescent immunoassay has been developed for the detection of CHL. Owing to the amplification of silver nanoparticles, high sensitivity for CHL could be achieved with a low detection limit of 7.6×10(-9) g mL(-1) and a wide linear dynamic range of 1.0×10(-8)-1.0×10(-6) g mL(-1). This method has also been successfully applied to determine CHL in milk and honey samples with a good recoveries (92% and 102%, 99% and 107% respectively), indicating that the method is feasible for the determination of CHL in real milk and honey samples. The labeling procedure is simple, convenient and fast, superior to previously reported labeling procedures. The immunoassay is also simple, fast, sensitive and selective. It is of application potential for the determination of CHL in foodstuffs. PMID:24491787

Yu, Xiuxia; He, Yi; Jiang, Jie; Cui, Hua

2014-02-17

88

Two-phase flow cell for chemiluminescence and bioluminescence measurements  

SciTech Connect

A new approach to two-phase CL (chemiluminescence) measurements is reported. A magnetically stirred reagent phase is separated from the analyte phase by a dialysis membrane so that only smaller molecules can go from one phase to the other. The system is designed so that the analyte phase flows through a spiral groove on an aluminum block that is flush against the dialysis membrane. As solution flows through the spiral grove, analyte diffuses into the reagent phase where it reacts to produce light. A simple model is developed to predict how this system will behave. Experimentally, the system is evaluated by using the luminol reaction catalyzed by peroxidase, the firefly reaction, and the bacterial bioluminescence reaction. 10 references, 4 tables, 6 figures.

Mullin, J.L.; Seitz, W.R.

1984-01-01

89

Gold nanoparticles - enhanced capillary electrophoresis- chemiluminescence assay of trace uric acid  

PubMed Central

A sensitive method based on gold nanoparticle-enhanced capillary electrophoresis-chemiluminescence detection was developed for quantifying uric acid in serum. In this work, gold nanoparticles were added into the running buffer of capillary electrophoresis to catalyze the post-column chemiluminescence reaction between luminol and hydrogen peroxide, achieving highly efficient chemiluminescence emission. Negative peaks were produced due to the inhibitory effects on chemiluminescence emission from uric acid eluted from the electrophoretic capillary. The decrease in chemiluminescence intensity was proportional to the concentration of uric acid in the range of 2.5 × 10?7 ~ 1.0 × 10?5 M. Detection limit was 4.6 × 10?8 M uric acid. Ten human serum samples were analyzed by the presented method. Serum level of uric acid was found to be in the range from 204 to 324 ?M for healthy subjects (n=5), and from 464 to 497 ?M for diabetic patients (n=5). The two groups were significantly different (p < 0.05). The results suggested a potential application of the proposed assay in rapid primary diagnosis of diseases such as diabetes.

Zhao, Shulin; Lan, Xuehua; Liu, Yi-Ming

2009-01-01

90

A Greener Chemiluminescence Demonstration  

ERIC Educational Resources Information Center

Because they are dramatic and intriguing, chemiluminescence demonstrations have been used for decades to stimulate interest in chemistry. One of the most intense chemiluminescent reactions is the oxidation of diaryl oxalate diesters with hydrogen peroxide in the presence of a fluorescer. In typical lecture demonstrations, the commercially…

Jilani, Osman; Donahue, Trisha M.; Mitchell, Miguel O.

2011-01-01

91

Sulfide chemiluminescence detection  

Microsoft Academic Search

A method of chemiluminescently determining a sulfide which is either hydrogen sulfide or methyl mercaptan by reacting the sulfide with chlorine dioxide at low pressure and under conditions which allow a longer reaction time in emission of a single photon for every two sulfide containing species, and thereafter, chemiluminescently detecting and determining the sulfide. The invention also relates not only

Stanford R. Spurlin; Edward S. Yeung

1985-01-01

92

Sulfide chemiluminescence detection  

Microsoft Academic Search

A method is described for chemiluminescently determining a sulfide which is either hydrogen sulfide or methyl mercaptan by reacting the sulfide with chlorine dioxide at low pressure and under conditions which allow a longer reaction time in emission of a single photon for every two sulfide containing species, and thereafter, chemiluminescently detecting and determining the sulfide. The invention also relates

S. R. Spurlin; E. S. Yeung

1985-01-01

93

Highly sensitive chemiluminescent analysis of residual bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies and peroxyoxalate-glyoxaline-PHPPA dimer chemiluminescent system in vaccines.  

PubMed

Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vaccines. A typical "sandwich type" immunoassay was used. Reaction of 3-(4-hydroxyphenyl propionate) (PHPPA) with hydrogen peroxide-urea, catalyzed by HRP, produced fluorescence of 3-(4-hydroxyphenyl propionate) dimer, which was detected by chemiluminescence analysis with the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H(2)O(2)-glyoxaline-PHPPA dimer chemiluminescent system. This method exhibited high performance with a linear correlation between response and amount of bovine serum albumin (BSA) in the range 0.1 to 100.0 ng mL(-1) (r?=?0.9988), and the detection limit was 0.03 ng mL(-1) (S/N?=?3). Intra- and interassay coefficient variations were all lower than 9.0% at three concentrations (1.0, 20.0, and 80.0 ng mL(-1)). The proposed method has been used for successful analysis of the amount of residual BSA in vaccines. The results obtained compared well with those obtained by conventional colorimetric ELISA and luminol chemiluminescent ELISA. PMID:22328250

Xue, Pan; Zhang, Kui; Zhang, Zhujun; Li, Yun; Liu, Feng; Sun, Yuanjie; Zhang, Xiaoming; Song, Chaojun; Fu, Aihua; Jin, Boquan; Yang, Kun

2012-03-01

94

Diagnostic validity of the chemiluminescent method compared to polymerase chain reaction for hepatitis B virus detection in the routine clinical diagnostic laboratory  

PubMed Central

Background: Hepatitis B virus (HBV) is the most common significant chronic viral infection world-wide. Hepatitis B surface antigen (HBsAg) has been the principal target for laboratory testing to identify active infection by HBV. We aimed to find out diagnostic validity of the Liaison chemiluminescent method compared to the polymerase chain reaction (PCR) method for HBV detection in the routine clinical diagnostic laboratory. Materials and Methods: From 350 patients suspicious of having infection with HBV, serum samples were separated and used for testing HBsAg by two methods of Liaison chemiluminescent immunoassay, with HBsAg confirmatory test and PCR method. Results: According to the PCR results as assumed as gold standard method with 100% sensitivity and specificity, detection rate sensitivity of chemiluminescent with confirmatory test was 96% and its specificity was 100%, and for chemiluminescent without confirmatory test sensitivity and specificity were 100% and 70%, respectively. Also for chemiluminescent with confirmatory test, positive predictive value (PPV) was 100% and its negative predictive value (NPV) was 97%, compared to chemiluminescent without confirmatory test with PPV and NPV equal to 71% and 100%, respectively. Conclusions: It is possible to conclude that in the majority of the HBV cases, the diagnostic value of chemiluminescent method compared to the PCR method is acceptable, except in low indexes positive cases that need further investigation with the PCR method.

Khadem-Ansari, Mohammad-Hassan; Omrani, Mir-Davood; Rasmi, Yousef; Ghavam, Arsalan

2014-01-01

95

Highly sensitive homogenous chemiluminescence immunoassay using gold nanoparticles as label.  

PubMed

Homogeneous immunoassay is becoming more and more attractive for modern medical diagnosis because it is superior to heterogeneous immunoassay in sample and reagent consumption, analysis time, portability and disposability. Herein, a universal platform for homogeneous immunoassay, using human immunoglobulin G (IgG) as a model analyte, has been developed. This assay relies upon the catalytic activity of gold nanoparticles (AuNPs) on luminol-AgNO3 chemiluminescence (CL) reaction. The immunoreaction of antigen and antibody can induce the aggregation of antibody-functionalized AuNPs, and after aggregation the catalytic activity of AuNPs on luminol-AgNO3 CL reaction is greatly enhanced. Without any separation steps, a CL signal is generated upon addition of a trigger solution, and the CL intensity is directly correlated to the quantity of IgG. The detection limit of IgG was estimated to be as low as 3pg/mL, and the sensitivity was better than that of the reported AuNPs-based CL immunoassay for IgG. PMID:24835732

Luo, Jing; Cui, Xiang; Liu, Wei; Li, Baoxin

2014-10-15

96

On-line chemiluminescence determination of mitoxantrone by capillary electrophoresis.  

PubMed

A novel capillary electrophoresis (CE) with chemiluminescence (CL) detection method for the determination of mitoxantrone (MTX) has been developed, which based on the CL reaction of potassium ferricyanide with luminol in sodium hydroxide medium sensitized by MTX. Under optimum analytical conditions, MTX is determined over the range of 7.0×10(-8)-1.0×10(-6)M with a detection limit of 1.0×10(-8)M. The relative standard deviation (RSD) was 3.7%, 2.6% and 3.0% for 7.0×10(-8), 5.0×10(-7) and 1.0×10(-6)M MTX (n=11), respectively. In laboratory-built CE-CL apparatus, the proposed method has been applied to determination of MTX in commercial drug and spiked in human urine and plasma with satisfactory results. PMID:20817570

Han, Suqin; Wang, Huili

2010-10-15

97

Infrared chemiluminescence from the reactions F + HBr, F + H 2Se, and Cl + H 2Se  

NASA Astrophysics Data System (ADS)

Infrared chemiluminescence from HF and HCl has been observed and yielded vibrational and rotational population distributions for the reactions F + HBr, F + H 2Se, and Cl + H 2Se. Evaluation of the spectra recorded by a commercial Fourier-transform spectrometer under low-flow conditions gave the following relative vibrational populations: for F - HBr. N? = 1 : N? = 2 : N? = 3 : N? = 4 = 0.45 : 0.31 : 0.13 : 0.11: for F + H 2Se, N? = 1 : N? = 2 : N? = 3 : N? = 4 : N? = 5 = 0.29 : 0.35 : 0.24 : 0.09 : 0.03: for Cl + H 2Se, N? = 1 : N? = 2 : N? = 3 = 0.40 : 0.51 : 0.09. All three vibrational surprisal plots show a significant deviation from linearity. Neglecting the contributions from N? = 0, the total energy is partitioned into vibration and rotation as follows: < fV> = 0.49 and < fR> = 0.09 for F + HBr, < fV> = 0.41 and < fR> = 0.07 for F + H 2Se, < fV> = 0.53 and < fR> = 0.10 for Cl + H 2Se. Inclusion of estimates for N? = 0 gives the more realistic values < fV> = 0.24, 0.34, and 0.49 respectively. Whereas 9 ± 3% of the collisions between F + HBr yield Br in the excited 2P 1/2 state, no rovibrationally excited HSe fragments were detected in the two other systems. Consistent values for the bond dissociation energy D00(H?SeH) = 329 ± 5 kJ/mol and the enthalpy of formation ? H100 (HSe) = 137 ± 5 kJ/mol are derived from the highest observed HCl and HF levels.

Dill, B.; Heydtmann, H.

1983-11-01

98

Continuous measurement of nitrous acid (HONO) in indoor environment using a diffusion scrubber and chemiluminescence method  

NASA Astrophysics Data System (ADS)

Recent study has demonstrated that the use of combustion appliances in indoor environments, e.g., gas stoves and heaters, results in significant concentrations of NO2 and nitrous acid (HONO). Indoor HONO is formed by both direct emissions from combustion processes and the heterogeneous reaction of NO2 with water vapor on surfaces present indoors. In this study in-situ instrument was constructed for measuring HONO concentration in both indoor and outdoor environments, utilizing diffusion scrubber and peroxynitrite-induced luminol chemiluminescent methods. We measured the HONO concentration under the conditions existing in living room of an apartment, along with NO, NO2, temperature, and relative humidity, to investigate the sources, chemical transformation, and lifetimes of nitrogen oxides and HONO. Some experiments investigated the emissions and transformations of nitrogen species from operation of unvented or vented gas appliance. Measurement data of NO, NO2, and HONO will be reported, and formation pathway of the HONO under the experimental conditions will also be discussed. In addition to measurement of indoor HONO, comparison of HONO measurements by luminol chemiluminescence and annular denuder integrated samples was made in outdoor environment. HONO in ambient air was sampled with annular denuders (Teflon-coated PM2.5 cyclone inlet followed by two Na2CO3-coated denuders coupled in series) operated at 16.7 L/min. Acknowledgement This study was supported by grant No. (# R01-2005-000-10775-0) from the Basic Research Program of the Korea Science and Engineering Foundation (KOSEF).

Park, S.; Hong, J.; Lee, J.; Cho, S.

2006-12-01

99

Thermal Chemistry of Cyclopropyl Substituted Malonyl Peroxides. A New Chemiluminescent Reaction.  

National Technical Information Service (NTIS)

This document investigates the thermal properties and chemiluminescent behavior of 4-cyclo-propyl-4-methyl-1,2-dioxolane-3,5-dione (2) and 4-spiro ((2'-spirocyclopropyl)-cyclohexyl)-1,2-dioxolane-3,5-dione. The gross chemical properties of these malonyl p...

M. J. Darmon G. B. Schuster

1982-01-01

100

A novel microarray chemiluminescence method based on chromium oxide nanoparticles catalysis for indirect determination of the explosive triacetone triperoxide at the scene.  

PubMed

Chromium oxide (Cr(2)O(3)) nanoparticles were found to greatly enhance the chemiluminescence (CL) of the luminol-hydrogen peroxide (H(2)O(2)) system. A novel microarray CL method was originally developed for the detection of triacetone triperoxide (TATP). The novel CL method was based on the outstanding catalytic effect of a Cr(2)O(3) nanoparticle array on the CL reaction between lower concentrations of luminol and H(2)O(2), which come from hydrolysis of TATP vapor. The calibration curve of H(2)O(2) was linear over a range of 1.0 × 10(-8) to 3.0 × 10(-5) M with a detection limit of 1.6 × 10(-9) M (R(2) = 0.9992, n = 12). The CL method has the advantages of being sensitive, selective, simple, time-saving, high-throughput, and shows good reproducibility. Therefore, these merits would make it easily popular. PMID:23353817

Li, Xiaohua; Zhang, Zhujun; Tao, Liang

2013-03-01

101

Chemiluminescent and Bioluminescent Techniques  

Microsoft Academic Search

intermediates that decay to a ground state with the emission of light. BL is a special type of CL found in nature, in which the light emission is facilitated by an enzyme(luciferase) or a photoprotein. The overall quan- tum yield of a CL reaction (the number of photons emitted\\/number of molecules reacting) is generally in the range 1-10% (e.g., luminol

Larry J. Kricka

102

Polymorphonuclear leukocyte chemiluminescence induced by formylmethionyl-leucyl-phenylalanine and phorbol myristate acetate: Effects of catalase and superoxide dismutase  

Microsoft Academic Search

When polymorphonuclear leukocytes (PMNL) interact with the soluble stimuli FMLP or PMA, the cells increase their production of oxidative metabolites. This increased production can be measured as luminol amplified light emission or chemiluminescence (CL). The chemiluminescence of human PMNL has been investigated, and it was found that the chemoattractant FMLP induced a bimodal response with a sharp peak of activity

C. Dahlgren

1987-01-01

103

Glow-Discharge Shock Tube for Studying Chemiluminescent, Surface-Catalytic, and Gas-Phase Reaction Rates; Temperature Dependence of NO&sngbnd;O and CO&sngbnd;O Chemiluminescence  

Microsoft Academic Search

The ordinary glow-discharge tube has been used extensively to study both surface and gas-phase recombination rates, as well as chemiluminescent reactions, typically at room temperature. The combination of a glow-discharge flow tube with a shock-tube driver to provide a considerable extension of atom fluxes, temperatures, and densities for the study of these kinetic processes is described. In operation, the ordinary

R. A. Hartunian; W. P. Thompson; E. W. Hewitt

1966-01-01

104

Studies of Chemiluminescence in Boron Atom Reactions with O2, SO2, N2O, NO2 and H2O2.  

National Technical Information Service (NTIS)

Chemiluminescence has been observed in the single collision reactions of boron atoms with O2, SO2, N2O, NO2, and H2O2. Experiments were performed in a beam-gas apparatus using photon counting to detect the optical signals. The electronically excited ((A s...

J. DeHaven M. T. O'Connor P. Davidovits

1980-01-01

105

Chemiluminescence determination of trimetazidine via inducing the aggregation of gold nanoparticles.  

PubMed

A simple, rapid and sensitive chemiluminescence (CL) method combined with flow injection analysis was developed for the determination of trimetazidine. Trimetazidine was found to significantly increase the CL signal arising from N-bromosuccinimide-luminol reaction in the presence of gold nanoparticles. The enhanced CL intensity was proportional to trimetazidine concentration in the range of 0.01-5.0 ?g/mL, with a limit of detection (3 sb) of 6.7 ng/mL. The relative standard deviation was 2.8% for 11 repetitive measurements of 0.1 ?g/mL trimetazidine solution. The practicality of the method was evaluated by determining trimetazidine in pharmaceutical formulations and in spiked human serum samples. Moreover, the possible CL reaction mechanism was also discussed. PMID:23747432

Li, Jiao; Quan, Jie; Du, Jianxiu; Liu, Mei

2013-10-01

106

Photodynamic action of some sensitizers by photooxidation of luminol  

NASA Astrophysics Data System (ADS)

We report the development of a novel simple experimental method which allows the comparison of new photosensitizers based on their production of reactive oxygen species. A high-performance liquid chromatography (HPLC) assay permits the monitoring of several substances (sensitizer, reactant and oxidized end product) simultaneously on a single chromatogram. Photoreactions were monitored throughout their course by the HPLC assay surveying the sensitizers' efficiency of singlet oxygen production by the oxidative decomposition of luminol. Several photosensitizers were tested: Rose Bengal, Methylene Blue, Protoporphyrin IX, Photosan III, Photofrin, Hypericin and Pseudohypericin. Additionally, photoreactions were monitored by a standard pO2 detection system. The measurements of the two detection methods were strongly correlated. Rose Bengal proved to be the most efficient photosensitizer, clearly decreasing the luminol concentration and causing a corresponding increase in aminophthalic acid. Our experiments show that when factors necessary for photochemical reactions are absent or are blocked (antioxidants), no reaction can be detected.

Wierrani, Franz; Kubin, Andreas; Loew, Hans Günter; Henry, Michael; Spängler, Babette; Bodner, Klaus; Grünberger, Werner; Ebermann, Robert; Alth, Gerhart

2002-09-01

107

Electrogenerated Chemiluminescence. X. Alpha, Beta, Gamma, Delta-Tetraphenylporphin Chemiluminescence.  

National Technical Information Service (NTIS)

Electrogenerated chemiluminescence (ecl) resulting from the reaction of alpha, beta, gamma, delta tetraphenylporphin (TPP) anion radical with the cation radical of TPP, rubrene, or 10-methylphenothiazine in methylene chloride solutions is described. The e...

A. J. Bard C. P. Keszthelyi N. E. Tokel

1972-01-01

108

Synthesis and characterizations of iso-luminol-functionalized, tadpole-shaped, gold nanomaterials.  

PubMed

Iso-luminol functionalized gold nanomaterials were synthesized in high yield by a simple seeding approach, using the chemiluminescent reagent iso-luminol as reductant in the presence of HAuCl(4), AgNO(3) and cetyltrimethylammonium bromide (CTAB). The morphology of as-prepared gold nanoparticles was characterized by transmission electron microscopy and UV-vis spectroscopy, showing that gold nanotadpoles (AuNTps) were obtained. Subsequent experiments revealed that the amounts of seed colloids and AgNO(3) and the concentrations of iso-luminol and CTAB in the growth solution play critical roles in the formation of well-shaped AuNTps. The surface state of AuNTps was characterized by UV-vis spectroscopy and fluorescence spectroscopy, indicating that iso-luminol and its oxidation product, 4-aminophthalate, coexisted on the surface of AuNTps. The CL behaviour was studied by static injection CL experiments, demonstrating that AuNTps were of CL activity. Finally, the growth mechanism of AuNTps was also discussed. PMID:22223619

Li, Fang; Tian, Dayong; Cui, Hua

2013-01-01

109

Determination of uric acid in human urine and serum by capillary electrophoresis with chemiluminescence detection.  

PubMed

A simple and sensitive method based on capillary electrophoresis (CE) with chemiluminescence (CL) detection has been developed for the determination of uric acid (UA). The sensitive detection was based on the enhancement effect of UA on the CL reaction between luminol and potassium ferricyanide (K3[Fe(CN)6]) in alkaline solution. A laboratory-built reaction flow cell and a photon counter were deployed for the CL detection. Experimental conditions for CL detection were studied in detail to achieve a maximum assay sensitivity. Optimal conditions were found to be 1.0 x 10(-4) M luminol added to the CE running buffer and 1.0 x 10(-4) M K3[Fe(CN)6] in 0.2 M NaOH solution introduced postcolumn. The proposed CE-CL assay showed good repeatability (relative standard deviation [RSD]=3.5%, n=11) and a detection limit of 3.5 x 10(-7) M UA (signal/noise ratio [S/N]=3). A linear calibration curve ranging from 6.0 x 10(-7) to 3.0 x 10(-5) M UA was obtained. The method was evaluated by quantifying UA in human urine and serum samples with satisfactory assay results. PMID:18445465

Zhao, Shulin; Wang, Jianshi; Ye, Fanggui; Liu, Yi-Ming

2008-07-15

110

Molecular beam chemiluminescence studies of the NO + O/sub 3/ reaction and modeling of global NO/sub 2/ distribution  

SciTech Connect

The results of a crossed molecular beam study of the chemiluminescent reaction NO + 0/sub 3/ ..-->.. NO/sub 2/ + 0/sub 2/ are discussed. The chemiluminescence as a function of collision energy and an excitation function were obtained using a translationally cooled supersonic NO beam. An investigation into the role of the internal energy states using an effusive NO beam and a supersonic O/sub 3/ beam has been presented. The results show that chemiluminescence enhancement occurs when high and low temperature NO experiments are compared. The role that other energy modes may have is discussed. The observed enhancement is consistent with the concept that the chemiluminescence cross section increases with NO molecular rotation for low J states. The second part discusses the role of NO/sub 2/ in preserving a global ozone balance. NO/sub 2/ vertical profiles based on Noxon's (1979) column measurements were derived. The method of instantaneous rates was used to calculate the rate of ozone production and destruction by O/sub x/ and NO/sub x/ on a grid that covered the entire globe. The results were presented as a function of altitude and latitude in contour plots.

Kowalczyk, M.

1980-11-01

111

Quenching-Chemiluminescence Determination of Trace Amounts of l-Tyrosine Contained in Dietary Supplement by Chemiluminescence Reaction of an Iron-Phthalocyanine Complex  

PubMed Central

The chemiluminescence (CL) signal immediately appeared when a hydrogen peroxide solution was injected into an iron-phthalocyanine tetrasulfonic acid (Fe-PTS) aqueous solution. Moreover, the CL intensity of Fe-PTS decreased by adding l-tyrosine. Based on these results, the determination of trace amounts of l-tyrosine was developed using the quenching-chemiluminescence. The calibration curve of l-tyrosine was obtained in the concentration range of 2.0 × 10?7?M to 2.0 × 10?5?M. Moreover, the relative standard deviation (RSD) was 1.63 % (n = 5) for 2.0 × 10?6?M l-tyrosine, and its detection limits (3?) were 1.81 × 10?7?M. The spike and recovery experiments for l-tyrosine were performed using a soft drink. Furthermore, the determination of l-tyrosine was applied to supplements containing various kinds of amino acids. Each satisfactory relative recovery was obtained at 98 to 102%.

Ohtomo, Takao; Igarashi, Shukuro; Takagai, Yoshitaka; Ohno, Osamu

2012-01-01

112

On the luminescence of luminol in DMSO in the presence of potassium superoxide-18-crown-6-ether and fluorescein  

Microsoft Academic Search

Luminol solution in DMSO in the presence of [18C6…K]+ O2? supramolecular complex (achieved from KO2 and 18-Crown-6 (18C6)-ether) is chemiluminescent, and its intensity depends on the complex concentration. Using fluorescein (Fl) as an energy acceptor in this system, the luminescence energy transfer process from chemically excited species, aminophtalate dianion, to Fl could be evidenced. On the basis of Förster theory,

Mariana Voicescu; Marilena Vasilescu; Titus Constantinescu; Aurelia Meghea

2002-01-01

113

Decrease of neutrophils chemiluminescence during exposure to low-power laser infrared radiation  

NASA Astrophysics Data System (ADS)

The neutrophil is the cell in which phagocyting and transforming of some exogeneous agents results in marked stimulation of nonmitochondrial respiratory chain activity (respiratory burst). In our experiment we focused on determining the level of chemiluminescence (CL) of stimulated neurotrophils during and after irradiation, measuring the photon emission intensity in 6 second's intervals. We used Ga-Al-As pulsed laser (wavelength 904 nm, mean power 8,9 mW, Alpha-Electronics GmbH, Germany) which was placed over the tube containing the suspension of guinea pig peritoneal neurotrophils (2X106 cells/ml). The sensitivity range of used photomultiplier (9514s, THORN EMI, Middlesex, England) was 300-600 nm, which allowed us to measure the CL of neutrophils while being irradiated. The neutrophils were stimulated by phorbol myristate acetate (PMA) and CL intensified by luminol. The decay of luminol-dependent CL of neutrophils may be described by hyperbolic function curve. We switched the laser radiation on for 20 s, 60 s and 300 s and each time we observed the same reaction: the about 20% decrease of intensity of CL immediately after beginning the irradiation. The CL remained on decreased level during the whole period of irradiation reaching immediately the level of CL intensity characteristic for decay curve (20% increase), just after switching off the laser. Only after the longest irradiation time (300 s) we observed CL being higher and inconsistent with decay curve for several minutes. The type of reaction was always the same, regardless to the point of CL decay curve at which laser radiation was applied. The same changes of Cl we obtained irradiating the enzymatic system: horseradish peroxidase (HRP)-luminol - H2O2.

Czuba, Zenon P.; Adamek, Mariusz; Krol, Wojciech; Sieron, Aleksander; Cieslar, Grzegorz

1995-01-01

114

Enhanced chemiluminescent resonance energy transfer in hollow calcium phosphate nanoreactors and the detection of hydrogen peroxide  

Microsoft Academic Search

Chemiluminescent nanoreactors (nano-CRET) that exhibit unusually efficient chemiluminescence resonance energy transfer to a co-encapsulated fluorophore when reacted with hydrogen peroxide are described. The nanoparticles have a mean size of 150 ± 20 nm and consist of a calcium phosphate shell surrounding a hollow core that is filled with a solution containing luminol, haematin and fluorescein. Steady state and stopped-flow fluorescence

P A Wingert; H Mizukami; A E Ostafin

2007-01-01

115

Chemiluminescence of benzoic and cinnamic acids, and flavonoids in the presence of aldehyde and hydrogen peroxide or hydroxyl radical by fenton reaction  

Microsoft Academic Search

Light emission (chemiluminescence; CL) was observed by exciting phenolic compounds with hydrogen peroxide (H2O2) or hydroxyl radical (HO·), formed from H2 and FeCl2 by the Fenton reaction. The intensity of the CL of benzoic acid derivatives in the presence of (H2 was in the order of gallic acid > caffeic acid > 3,5-dimethoxy-4-hydroxycinnamic acid > isovanillic acid = syringic acid.

Yumiko Yoshiki; Kazuyoshi Okubo; Masamichi Onuma; Kiharu Igarashi

1995-01-01

116

High-sensitivity chemiluminescence detection of cytokines using an antibody-immobilized CMOS image sensor  

NASA Astrophysics Data System (ADS)

In this study, we used a Complementary Metal Oxide Semiconductor (CMOS) image sensor with immobilizing antibodies on its surface to detect human cytokines, which are activators that mediate intercellular communication including expression and control of immune responses. The CMOS image sensor has many advantages over the Charge Couple Device, including lower power consumption, operation voltage, and cost. The photodiode, a unit pixel component in the CMOS image sensor, receives light from the detection area and generates digital image data. About a million pixels are embedded, and size of each pixel is 3 x 3 ?m. The chemiluminescence reaction produces light from the chemical reaction of luminol and hydrogen peroxide. To detect cytokines, antibodies were immobilized on the surface of the CMOS image sensor, and a sandwich immunoassay using an HRP-labeled antibody was performed. An HRP-catalyzed chemiluminescence reaction was measured by each pixel of the CMOS image sensor. Pixels with stronger signals indicated higher cytokine concentrations; thus, we were able to measure human interleukin-5 (IL-5) at femtomolar concentrations.

Hong, Dong-Gu; Joung, Hyou-Arm; Kim, Sang-Hyo; Kim, Min-Gon

2013-05-01

117

3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein.  

PubMed

Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10'-phenothiazinyl)propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09 pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5 ng mL(-1). The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits. PMID:24054611

Sakharov, Ivan Yu; Demiyanova, Alexandra S; Gribas, Anastasia V; Uskova, Natalia A; Efremov, Evgeny E; Vdovenko, Marina M

2013-10-15

118

Reusable light-emitting-diode induced chemiluminescence aptasensor for highly sensitive and selective detection of riboflavin.  

PubMed

A novel reusable chemiluminescence aptasensor was developed based on aptamer recognition coupled with light-emitting-diode induced chemiluminescence (LED-CL) detection. The sensing approach was based on the design that the model analyte riboflavin (Rf) in sample solutions was captured by the immobilized aptamers and then eluted simply with alkaline luminol solution to catalyze the CL reaction between luminol and dissolved oxygen under high power LED irradiation. This design allowed a very simple (branch-free) flow way for the CL sensing system. The CL intensity versus the Rf concentration was linear in the range from 0.03 to 5ngmL(-1) with a limit of detection (LOD) down low to 8pgmL(-1). Without renewing the aptamer, the relative standard deviation (RSD) for seven consecutive detections is 2.33%; the sensor also showed good stability without performance deterioration after >100 times use. Up to 10000-fold of K(+), Na(+), Ca(2+), Mg(2+), and Fe(3+), 5000-fold for glucose and bovine serum albumin, 1000-fold of uric acid, 1500-fold of ascorbic acid (added with Fe(3+)), 100-fold of flavin mononucleotide and 200-fold of flavin adenine dinucleotide caused no significant interference with the determination of 0.5ngmL(-1) Rf. The sensor was applied for analysis of Rf in urine and food samples with the recovery of 94-103%. The advantages of reusability, simplicity, high sensitivity and selectivity provided by the LED-CL aptasensor will make it a good alternative tool for biological and food analysis. PMID:23298628

Xu, Shuxia; Zhang, Xinfeng; Liu, Weiwei; Sun, Yonghua; Zhang, Heliang

2013-05-15

119

Homogeneous assay of target molecules based on chemiluminescence resonance energy transfer (CRET) using DNAzyme-linked aptamers.  

PubMed

We have designed a single-stranded DNAzyme-aptamer sensor for homogeneous target molecular detection based on chemiluminescence resonance energy transfer (CRET). The structure of the engineered single-stranded DNA (ssDNA) includes the horseradish peroxidase (HRP)-like DNAzyme, optimum-length linker (10-mer-length DNA), and target-specific aptamer sequences. A quencher dye was modified at the 3' end of the aptamer sequence. The incorporation of hemin into the G-quadruplex structure of DNAzyme yields an active HRP-like activity that catalyzes luminol to generate a chemiluminescence (CL) signal. In the presence of target molecules, such as ochratoxin A (OTA), adenosine triphosphate (ATP), or thrombin, the aptamer sequence was folded due to the formation of the aptamer/analyte complex, which induced the quencher dye close to the DNAzyme structure. Consequently, the CRET occurred between a DNAzyme-catalyzed chemiluminescence reaction and the quencher dye. Our results showed that CRET-based DNAzyme-aptamer biosensing enabled specific OTA analysis with a limit of detection of 0.27ng/mL. The CRET platform needs no external light source and avoids autofluorescence and photobleaching, and target molecules can be detected specifically and sensitively in a homogeneous manner. PMID:24658027

Mun, Hyoyoung; Jo, Eun-Jung; Li, Taihua; Joung, Hyou-Arm; Hong, Dong-Gu; Shim, Won-Bo; Jung, Cheulhee; Kim, Min-Gon

2014-08-15

120

Flow-injection analysis of hydrogen peroxide based on carbon nanospheres catalyzed hydrogen carbonate-hydrogen peroxide chemiluminescent reaction.  

PubMed

A flow-injection chemiluminescence (CL) system with high sensitivity, selectivity, rapidity, and reproducibility is proposed for the determination of hydrogen peroxide (H(2)O(2)) in water samples. The system is based on the reaction of hydrogen peroxide and hydrogen carbonate solution. Carbon nanospheres (CNSs) prepared from aqueous glucose solution are used to enhance the weak CL. The CL intensity was found to be directly proportional to the concentration of H(2)O(2) present in the sample solutions. The effects upon the CL of several physicochemical parameters, including the concentration of the reagents, the mixing order of the reagents, flow rate, pH, particle size of CNSs and other relevant variables, were studied and optimized. The proposed method exhibited advantages in a larger linear range of 5.0 × 10(-8) to 3.0 × 10(-6) mol L(-1) and a lower limit of detection of 1.0 × 10(-9) mol L(-1) (S/N = 3). This method has been successfully applied to the evaluation of H(2)O(2) in tap water and snow water with recoveries from 80 to 110%. The relative standard deviation (RSD) was less than 8% for intra- and inter-assay precision. Based on the kinetic curve, the CL spectrum, fluorescence spectrum, UV-visible spectrum, and electron spin resonance (ESR) spectrum of NaHCO(3)-H(2)O(2)-CNSs system, a possible CL mechanism was proposed. Superoxide ion radical (?O(2)(-)) and hydroxide radical (?OH) were generated during the reaction of NaHCO(3) and H(2)O(2). They were the key intermediates for the production of hole-injected and electron-injected CNSs in the CL process. PMID:21384016

Chen, Hui; Lin, Ling; Lin, Zhen; Lu, Chao; Guo, Guangsheng; Lin, Jin-Ming

2011-05-01

121

Chemiluminescence of Acanthamoeba castellanii.  

PubMed Central

1. Chemiluminescence of Acanthomoeba castellanii in the presence of O2 was of similar intensity in organisms harvested early or late during exponential growth [when cyanide (1 mM) stimulates or inhibits respiration respectively]. 2. Cyanide (up to 1.5 mM) stimulated photoemission in both types of organism by 250--300 photons/s per 10(7) cells above the value observed under aerobic conditions. 3. 'Dibromothymoquinone' (2,5-dibromo-6-isopropyl-3-methyl-p-benzoquinone) (up to 80 microM) further increased chemiluminescence. 4. Similar responses were also demonstrated in whole homogenates and in subcellular fractions; 36% of the chemiluminescence was provided by a fraction sedimenting at 100000g-min, and 20% in that fraction that was non-sedimentable at 200000g-min. 5. Mitochondrial substrates (succinate, 2-oxoglutarate, NADH) in the presence or absence of ADP and Pi or peroxisomal substrates (glycollate, urate or ethanol) gave no increases in light emission by whole homogenates or in any of the fractions. 6. It is suggested that reactions responsible for production of chemiluminescence are those primarily producing superoxide anions and leading to lipid peroxidation and singlet-oxygen formation. Photoemission enhancement and superoxide dismutase inhibition showed similar cyanide concentration-dependencies.

Lloyd, D; Boveris, A; Reiter, R; Filipkowski, M; Chance, B

1979-01-01

122

Chemiluminescence response of human polymorphonuclear leukocytes induced by purified, latex attached Klebsiella fimbriae.  

PubMed

Type 1 fimbriae (T1F) and type 3 fimbriae (T3F) were isolated from Klebsiella species, purified, attached to latex beads and tested for their ability to stimulate human polymorphonuclear leukocyte (PMNL) oxidative activity. The luminol dependent chemiluminescence assay was used to evaluate the response of phagocytes. Latex particles coated with type 3 fimbriae (1-T3F) induced a significantly higher chemiluminescence response than those with type 1 fimbriae (1-T1F). Opsonization of 1-T1F with pooled human serum induced chemiluminescence responses which were statistically significantly enhanced as compared to opsonized 1-T3F and both kinds of non-opsonized fimbriae. PMID:1684498

Przondo-Mordarska, A; Ko, H L; Beuth, J; Gamian, A; Pulverer, G

1991-10-01

123

Synthesis and chemiluminescence of copolymers of 5-amino-8-vinyl-phthalazine-1,4(2H,3H)-dione with methyl methacrylate or styrene, and of alpha, omega-bis[5-amino-phthalazine-1,4(2H,3H)-dion-]8-yl alkanes [= alpha, omega-bis(6-luminyl) alkanes]: investigations on an intramolecular 'distance effect'.  

PubMed

Oligomers of 5-amino-8-vinyl-phthalazine-1,4(2H,3H)-dione exhibit about 0.05% of the chemiluminescence quantum yield of the corresponding 'monomer unit', i.e. 5-amino-8-ethyl-phthalazine-1,4(2H,3H)-dione which has a similar quantum yield to luminol. The quantum yields of copolymers of 5-amino-8-vinyl-phthalazine-1,4(2H,3H)-dione (1a) with methyl methacrylate or with styrene increase up to 1000-fold, relative to the quantum yield of oligomers of (1a). Thus the monomer units of methyl methacrylate or styrene appear to act as 'spacers' between the lumigenic groups. alpha, omega-Bis[(5-amino-phthalazine-1,4(2H,3H)-dion-)8-yl] alkanes show an analogue 'distance' effect: the chemiluminescence quantum yield increases with increasing alkane chain length. As the fluorescence of the corresponding amino phthalates (which are intermediates in the synthesis of the phthalazine diones) is only slightly influenced by the distance between the lumigenic groups it is suggested that a mainly chemical 'distance effect' is working here: the smaller the intramolecular distance between the hydrazide groups the more inhibition exists in respect of the oxidative reaction producing the luminol-type chemiluminescence. PMID:3503528

Gundermann, K D; Lieske, D; Haase, B; Hartmann-Azanza Baca, B

1987-09-01

124

Chemiluminescence analysis of oil oxidizing bacteria Actinetobacter calcoaceticus extracts: Effects of the extracts on pSoxS-lux biosensor  

Microsoft Academic Search

A comparative H2O2-luminol- and Fe(II)-induced chemiluminescence analysis of extracts of two strains of marine oil oxidizing bacteria Actinetobacter calcoaceticus cultivated either in the presence or absence of oil was carried out. Effects of these extracts on E. coli MG1655 biosensor (pSoxS-lux) were studed. Activation of H2O2-induced chemiluminescence in the presence of oil was observed. This suggests activation of free radical

I. S. Sazykin; V. N. Prokofiev; V. A. Chistyakov; M. A. Sazykina; V. V. Vnukov

2011-01-01

125

Chemiluminescence development after initiation of Maillard reaction in aqueous solutions of glycine and glucose: nonlinearity of the process and cooperative properties of the reaction system  

NASA Astrophysics Data System (ADS)

Nonenzymatic glycation of free or peptide bound amino acids (Maillard reaction, MR) plays an important role in aging, diabetic complications and atherosclerosis. MR taking place at high temperatures is accompanied by chemiluminescence (CL). Here kinetics of CL development in MR proceeding in model systems at room temperature has been analyzed for the first time. Brief heating of glycine and D-glucose solutions to t greater than 93 degrees Celsius results in their browning and appearance of fluorescencent properties. Developed In solutions rapidly cooled down to 20 degrees Celsius a wave of CL. It reached maximum intensity around 40 min after the reaction mixture heating and cooling it down. CL intensity elevation was accompanied by certain decoloration of the solution. Appearance of light absorbing substances and development of CL depended critically upon the temperature of preincubation (greater than or equal to 93 degrees Celsius), initial pH (greater than or equal to 11,2), sample volume (greater than or equal to 0.5 ml) and reagents concentrations. Dependence of total counts accumulation on a system volume over the critical volume was non-monotonous. After reaching maximum values CL began to decline, though only small part of glucose and glycin had been consumed. Brief heating of such solutions to the critical temperature resulted in emergence of a new CL wave. This procedure could be repeated in one and the same reaction system for several times. Whole CL kinetic curve best fitted to lognormal distribution. Macrokinetic properties of the process are characteristic of chain reactions with delayed branching. Results imply also, that self-organization occurs in this system, and that the course of the process strongly depends upon boundary conditions and periodic interference in its course.

Voeikov, Vladimir L.; Naletov, Vladimir I.

1998-06-01

126

Detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in water using a flow-through chemiluminescence microarray readout system.  

PubMed

Fast, sensitive, and especially, multianalyte test systems are currently of high interest for the monitoring and quality control of drinking water, since traditional microbiological methods are labor intensive and can take days until a response is achieved. In this study, the first flow-through chemiluminescence microarray was developed and characterized for the rapid and simultaneous detection of Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila in water samples using a semiautomated readout system. Therefore, antibody microarrays were produced on poly(ethylene glycol)-modified glass substrates by means of a contact arrayer. For capturing bacteria, species-specific polyclonal antibodies were used. Cell recognition was carried out by binding of species-specific biotinylated antibodies. Chemiluminescence detection was accomplished by a streptavidin-horseradish peroxidase (HRP) catalyzed reaction of luminol and hydrogen peroxide. The chemiluminescence reaction that occurred was recorded by a sensitive charge-coupled device (CCD) camera. The overall assay time was 13 min, enabling a fast sample analysis. In multianalyte experiments, the detection limits were 3 x 10(6), 1 x 10(5), and 3 x 10(3) cells/mL for S. typhimurium, L. pneumophila, and E. coli O157:H7, respectively. Quantification of samples was possible in a wide concentration range with good recoveries. The presented system is well suited for quick and automatic water analysis. PMID:18578502

Wolter, Anne; Niessner, Reinhard; Seidel, Michael

2008-08-01

127

Sensitive chemiluminescence immunoassay by capillary electrophoresis with gold nanoparticles.  

PubMed

This technical note describes a new chemiluminescence immunoassay hyphenated to capillary electrophoresis (CE-based CL-IA) with gold nanoparticles (AuNPs) technique for biological molecules determination. AuNPs were used as a protein label reagent in the light of its excellent catalytic effect to the CL reaction of luminol and hydrogen peroxide. AuNPs conjugate with antibody (Ab) to form tagged antibody (Ab*), and then Ab* link to antigen (Ag) to produce an Ab*-Ag complex by a noncompetitive immunoreaction. The mixture of the excess Ab* and the Ab*-Ag complex was baseline separated and detected within 5 min under the optimized conditions. This new protocol was evaluated with human immunoglobulin G (IgG) as the target molecule. The calibration curve of IgG was in the range of 0.008-5 ?g/mL with a correlation coefficient of 0.995. The detection limit (S/N = 3) of IgG was 1.14 × 10(-3) ?g/mL (7.1 pmol/L, 0.39 amol). The proposed AuNPs enhanced CE-based CL-IA method was successfully applied for the quantification of IgG in human sera from patients. It proves that the present method could be developed into a new and sensitive biochemical analysis technique. PMID:21218847

Liu, Yan-ming; Mei, Lin; Liu, Li-juan; Peng, Long-fei; Chen, Yong-hong; Ren, Shu-wei

2011-02-01

128

Apparatus for use in sulfide chemiluminescence detection  

Microsoft Academic Search

A method of chemiluminescently determining a sulfide which is either hydrogen sulfide or methyl mercaptan by reacting the sulfide with chlorine dioxide at low pressure and under conditions which allow a longer reaction time in emission of a single photon for every two sulfide containing species, and thereafter, chemiluminescently detecting and determining the sulfide. The invention also relates not only

Stanford R. Spurlin; Edward S. Yeung

1987-01-01

129

Apparatus for use in sulfide chemiluminescence detection  

Microsoft Academic Search

A method is described for chemiluminescently determining a sulfide which is either hydrogen sulfide or methyl mercaptan by reacting the sulfide with chlorine dioxide at low pressure and under conditions which allow a longer reaction time in emission of a single photon for every two sulfide containing species, and thereafter, chemiluminescently detecting and determining the sulfide. The invention also relates

S. R. Spurlin; E. S. Yeung

1987-01-01

130

Micro-plate chemiluminescence enzyme immunoassay for aflatoxin B1 in agricultural products.  

PubMed

In this work, a micro-plate chemiluminescence enzyme immunoassay by antibody-coated for the determination of aflatoxin B1 (AFB1) in agricultural products has been established. Aflatoxin B1 antibody (AFB1-Ab) was adsorbed physically on polystyrene micro-plate hole as solid phase antibody, which took place immunity-reaction between antigen and antibody with AFB1 standard solution or samples by direct competition. Luminol-hydrogen peroxide chemiluminescence system catalyzed by horseradish peroxidase (HRP) with p-iodophenol enhancement was used as signal detecting system. The effects of several factors, including composition and pH of coating solution, dilution ratio and amount of antibody and enzyme labeled antigen, time of antibody-coating, incubation and chemiluminescence reaction, and other relevant variables upon the immunoassay were studied and optimized. The linear range of proposed method for AFB1 was 0.05-10.0 ng g(-1) with a correlative coefficient of -0.9997. The sensitivity of the proposed method was 0.01 ng g(-1). The RSDs of intra- and inter-assay were less than 12.2% and 10.0%, respectively. This method has been successfully applied to the evaluation of AFB1 in agricultural products with recoveries of 79.8%, 101.9% and 115.4% for low, middle and high concentration samples, respectively. It shows a good correlation with the commercial available ELISA kit for AFB1 with correlative coefficient of 0.9098 indicating that the established CLEIA method can be used to determine AFB1 in real samples. PMID:21315923

Fang, Luqiu; Chen, Hui; Ying, Xitang; Lin, Jin-Ming

2011-03-15

131

Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates  

PubMed Central

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.

Niazov, Angelica; Freeman, Ronit; Girsh, Julia; Willner, Itamar

2011-01-01

132

Fiber-Optic Chemiluminescent Biosensors for Monitoring Aqueous Alcohols and Other Water Quality Parameters  

NASA Technical Reports Server (NTRS)

A "reagentless" chemiluminescent biosensor and method for the determination of hydrogen peroxide, ethanol and D-glucose in water is disclosed. An aqueous stream is basified by passing it through a solid phase base bed. Luminol is then dissolved in the basified effluent at a controlled rate. Oxidation of the luminol is catalyzed by the target chemical to produce emitted light. The intensity of the emitted light is detected as a measure of the target chemical concentration in the aqueous stream. The emitted light can be transmitted by a fiber optic bundle to a remote location from the aqueous stream for a remote reading of the target chemical concentration.

Verostko, Charles E. (Inventor); Atwater, James E. (Inventor); Akse, James R. (Inventor); DeHart, Jeffrey L. (Inventor); Wheeler, Richard R. (Inventor)

1998-01-01

133

Gold nanoparticle-enhanced chemiluminescence detection for CE.  

PubMed

Introducing gold nanoparticles (AuNPs) to the running buffer further improved the sensitivity of luminol-H(2)O(2) chemiluminescence (CL) detection for CE. This has led to the development of sensitive CE-CL assays of biomedically interesting compounds. Epinephrine and norfloxacin were taken as the model analytes. Epinephrine inhibited light emission from the AuNP-catalyzed CL while norfloxacin enhanced it. The CE-CL assays had detection limits of 6.9x10(-9) M for epinephrine and 7.3x10(-9) M for norfloxacin. It was noted that in the absence of AuNPs no CE-CL analytical signal was produced by epinephrine at 4.0x10(-5) M or norfloxacin at 1.5x10(-3) M under similar experimental conditions. Fluorescence spectroscopic measurements showed that although the fluorescence excitation/emission maxima remained the same, the fluorescence lifetime of luminol increased significantly in the presence of AuNPs (tau(2) increased from 8.49+/-0.12 to 9.18+/-0.047 ns in a two-exponential fit), indicating that the excited states of luminol molecules were stabilized through the interaction between luminol molecules and AuNPs. Finally, quantitation of epinephrine and norfloxacin in biological samples such as human urine by using the present AuNP-enhanced CE-CL method was demonstrated. PMID:19309007

Zhao, Shulin; Niu, Tianxing; Song, Yaru; Liu, Yi-Ming

2009-03-01

134

Some Characteristics of Riboflavin Chemiluminescence.  

National Technical Information Service (NTIS)

Data are presented which describe some characteristics of riboflavin chemiluminescence in the dark in the presence of hydrogen peroxide and osmium trichloride. The reaction, in terms of the light intensity produced, is affected by the type of buffer, pH, ...

R. D. Towner H. A. Neufeld P. B. Shevlin

1969-01-01

135

Some Characteristics of Riboflavin Chemiluminescence.  

National Technical Information Service (NTIS)

Data are presented that describe some characteristics of riboflavin chemiluminescence in the dark in the presence of hydrogen peroxide and osmium trichloride. The reaction, in terms of the light intensity produced, is affected by type of buffer, pH, and c...

R. D. Towner H. A. Neufeld P. B. Shevlin

1969-01-01

136

Highly sensitive electrogenerated chemiluminescence biosensor in profiling protein kinase activity and inhibition using a multifunctional nanoprobe.  

PubMed

We presented a novel electrogenerated chemiluminescence (ECL) biosensor for monitoring the activity and inhibition of protein kinases based on signal amplification using enzyme-functionalized Au NPs nanoprobe. In this design, the biotin-DNA labeled glucose oxidase/Au NPs (GOx/Au NPs/DNA-biotin) nanoprobes, prepared by conjugating Au NPs with biotin-DNA and GOx, were bound to the biotinylated anti-phosphoserine labeled phosphorylated peptide modified electrode surface through a biotin-avidin interaction. The GOx assembled on the nanoprobe can catalyze glucose to generate H2O2 in the presence of O2 while the ECL reaction occurred in the luminol ECL biosensor. At a higher concentration of kinase, there are more nanoprobes on the electrode, which gives a higher amount of GOx at the electrode interface and thus higher electrocatalytic efficiency to the luminol ECL reaction. Therefore, the activity of protein kinases can be monitored by ECL with high sensitivity. Protein kinase A (PKA), an important enzyme in regulation of glycogen, sugar, and lipid metabolism in the human body, was used as a model to confirm the present proof-of-concept strategy. The as-proposed biosensor presents high sensitivity, low detection limit of 0.013 U mL(-1), wide linear range (from 0.02 to 40 U mL(-1)), and excellent stability. Moreover, this biosensor can also be used for quantitative analysis of kinase inhibition. On the basis of the inhibitor concentration dependent ECL signal, the half-maximal inhibition value IC50 of ellagic acid, a typical PKA inhibitor, was estimated, which is in agreement with those obtained using the conventional kinase assay. The simple and sensitive biosensor is promising in developing a high-through assay of in vitro kinase activity and inhibitor screening for clinic diagnostic and drug development. PMID:24491761

Liang, Ru-Ping; Xiang, Cai-Yun; Zhao, Hui-Fang; Qiu, Jian-Ding

2014-02-17

137

Bleach interference in forensic luminol tests on porous surfaces: more about the drying time effect.  

PubMed

As criminals try to avoid leaving clues at the scene of a crime, bloodstains are often washed away, but fortunately for investigators, they are difficult to eliminate completely. Porous surfaces easily retain blood traces, which are sometimes invisible to the naked eye. The reagent of choice for detecting latent blood traces on all types of surfaces is luminol, but its main disadvantage is a high degree of sensitivity to oxidising contaminants in the blood sample. If household bleach is used to clean bloodstains, presumptive tests are invalidated. Hypochlorites, however, are known to be unstable and deteriorate over time, and this feature could be of help in preventing household bleach-induced interference. Previous studies have evaluated the effect of the drying time on nonporous surfaces, but nothing has as yet been published about this effect on porous surfaces. Consequently, this paper reports on hypochlorite interference with luminol reagents used on this type of surface, evaluating the effects of drying time on the household bleach-luminol reaction, and ascertaining whether the drying procedure could be applied to prevent household bleach interference on bloodstained porous surfaces. The results indicate that the drying method may very well overcome household bleach interference in luminol reaction tests, if the investigation allows for an appropriate waiting time. PMID:19084679

Castelló, Ana; Francés, Francesc; Verdú, Fernando

2009-02-15

138

Imaging biocatalytic activity of enzyme-polymer spots by means of combined scanning electrochemical microscopy/electrogenerated chemiluminescence.  

PubMed

The purpose of this study was to develop a scanning electrochemical microscopy (SECM) and scanning electrogenerated chemiluminescence (SECL) setup to visualize the localized enzymatic activity using glucose oxidase as a model. Combination of SECM and electrogenerated chemiluminescence (ECL) was made possible by integrating a photomultiplier tube (PMT) within a SECM setup which is mounted on top of an inverted microscope. An enzyme-polymer spot formed on a glass slide and placed on top of the entrance window of the PMT was used as a model sample to evaluate the potential of the combined SECM/ECL setup. Hydrogen peroxide, which was locally generated by the glucose oxidase (GOx)-catalyzed reaction, reacted with oxidized luminol which was simultaneously electrochemically generated at the positioned SECM electrode tip. By using the phase-sensitive lock-in amplifier, the potential applied to the SECM tip was sinusoidally swept to invoke an associated oscillation of the ECL. Thus, sensitivity of SECL could be substantially enhanced. Images of the local immobilized enzyme activity obtained both by ECL and generator/collector (GC) mode of SECM were compared to elucidate the pathway in which the SECM and SECL signals are generated. PMID:19441829

Lei, Rong; Stratmann, Lutz; Schäfer, Dominik; Erichsen, Thomas; Neugebauer, Sebastian; Li, Na; Schuhmann, Wolfgang

2009-06-15

139

Chemiluminescence assay of mucosal reactive oxygen metabolites in inflammatory bowel disease.  

PubMed

Previous studies suggesting increased reactive oxygen metabolite (ROM) production in inflammatory bowel disease have been restricted to peripheral blood and isolated intestinal phagocytes. In the current study, chemiluminescence and the effect of various scavengers, enzymes, and enzyme inhibitors were used to show that ROMs account for the increased production of oxidants by colorectal mucosal biopsy specimens in inflammatory bowel disease. Luminol-amplified chemiluminescence was increased in active ulcerative colitis [macroscopic grade 1: 25 photons.mg-1.min.10(-3) (median), 8-47 (95% confidence intervals), n = 40; grade 2: 89, 65-156, n = 30; grade 3: 247, 133-562, n = 13] and Crohn's disease [mild: 9, 3-84, n = 6; severe: 105, 25-789 (range), n = 5] compared with normal-looking mucosa (ulcerative colitis: 0.8, 0.4-1.4, n = 22, P less than 0.01; Crohn's disease: 0.8, 0.1-2, n = 6, P less than 0.05) and controls (0.6, 0.04-1.4, n = 52, P less than 0.01). In ulcerative colitis, luminol chemiluminescence correlated with microscopic inflammation (Spearman's p = 0.74, P = 0.0001) and was decreased by sodium azide (-89%, P less than 0.05), taurine (-31%, P less than 0.05), catalase (-23%, P less than 0.05), and dimethyl sulfoxide (-29%, P less than 0.05). Superoxide dismutase and oxypurinol decreased lucigenin chemiluminescence in ulcerative colitis by -63% (P less than 0.05) and -27% (P less than 0.05), respectively. Luminol chemiluminescence correlated with lucigenin chemiluminescence (Spearman's rho = 0.72, P = 0.003). These results suggest that neutrophil-derived oxidants (superoxide, hydrogen peroxide, hydroxyl radical, and hypochlorite) are generated in colorectal mucosa in active inflammatory bowel disease and support the hypothesis that production of such metabolites by neutrophils is of major pathogenetic importance. PMID:1319369

Simmonds, N J; Allen, R E; Stevens, T R; Van Someren, R N; Blake, D R; Rampton, D S

1992-07-01

140

A Survey of Recent Literature on Chemiluminescence and Fluorescence in Solution.  

National Technical Information Service (NTIS)

This report represents a literature survey of the structural factors affecting direct and sensitized visible chemiluminescence in solution. Compilations of the quantitative and qualitative data for the important classes of direct chemiluminescent reaction...

E. G. Kayser T. N. Hall

1968-01-01

141

Chemiluminescence detection from sonodynamic action in vitro and in vivo  

NASA Astrophysics Data System (ADS)

In this work, chemiluminescence method was engaged for the first time to detect the active oxygen spices during sonocynamic action both in vitro and in vivo. We used CLA derivatives, which can efficiently react with singlet oxygen (1O2) or superoxide anion (O2-) to emit light, and luminol, which can be oxidized by a variety of free radicals to emit photons, to real-timely detect oxygen free radical formation in the sonosensitization of two sonosensitizer ATX-70 and HpD. The results show that 1O2 is involved in the sonosensitization. The mechanism of sonosensitizing was discussed. In vivo experiments, tumor imaging by sonodynamic chemiluminescence detection methods was established. This method could have potential applications in clinics for early-stage tumor diagnosis.

He, Yonghong; Xing, Da; Yan, Guihong; Ueda, Kenichi

2001-10-01

142

Sequential injection chemiluminescence immunoassay for nonionic surfactants by using magnetic microbeads.  

PubMed

A rapid and sensitive immunoassay based on a sequential injection analysis (SIA) using magnetic microbeads for the determination of alkylphenol polyethoxylates (APnEOs) is described. An SIA system was constructed from a syringe pump, a switching valve, a flow-through type immunoreaction cell equipped with a photon counting unit and a neodymium magnet. Magnetic beads, to which an anti-APnEOs monoclonal antibody was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of the magnetic beads in and from the immunoreaction cell were controlled by means of a neodymium magnet and adjusting the flow of a carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an anti-APnEOs monoclonal antibody immobilized on the magnetic beads with a sample APnEOs and a horseradish peroxidase (HRP)-labeled APnEOs in the same sample solution, and was based on the subsequent chemiluminscence reaction of HRP on the magnetic microbeads with a luminol solution containing hydrogen peroxide and p-iodophenol. The anti-APnEOs antibody was immobilized on the magnetic microbeads by coupling the antibody with the magnetic beads after activation of a carboxylate moiety on the surface of the magnetic beads that had been coated with a polylactic acid film. The antibody immobilized magnetic beads were introduced in the immunoreaction cell and trapped in it by the neodymium magnet, which was equipped beneath the immunoreaction cell. An APnEOs sample solution containing the HRP-labeled APnEOs at a constant concentration, and a luminol solution containing hydrogen peroxide and p-iodophenol were sequentially introduced into the immunoreaction cell, according to an SIA programmed sequence. Chemiluminescence emission was monitored by means of a photon counting unit located at the upper side of the immunoreaction cell by collecting the emitted light with a lens. A typical sigmoidal calibration curve was obtained, when the logarithm of the concentration of APnEOs was plotted against the chemiluminescence intensity as the number of photons in 100 ms using standard APnEOs sample solutions at various concentrations (0-1000 ppb) under optimum conditions. The lower detection limit defined as IC(80) is ca 10 ppb. The time required for analysis is less than 15 min per a sample. The present method was successfully applied to the determination of APnEOs in river water. PMID:17903471

Zhang, Ruiqi; Nakajima, Hizuru; Soh, Nobuaki; Nakano, Koji; Masadome, Takashi; Nagata, Kazumi; Sakamoto, Kazuhira; Imato, Toshihiko

2007-09-26

143

Different sensitivity of cells from tumor-bearing organisms to continuous-wave and pulsed laser radiation (lambda = 632.8 nm) evaluated by chemiluminescence test  

NASA Astrophysics Data System (ADS)

Chemiluminescence test results were used to evaluate the sensitivity of human blood and murine splenocytes to continuous-wave (CW) and pulsed He-Ne laser light. It is demonstrated that CW radiation has in our experimental conditions practically no effect on the luminol-amplified chemiluminescence of four models under study. The pulsed radiation had a week inhibiting effect on the samples from healthy organisms but inhibited markedly the chemiluminescence of samples from tumor-bearing organisms. The effect depended on duration of dark period between pulses. A transient local heating mechanism is proposed to explain the inhibition of activity of NADPH-oxidase.

Karu, Tina I.; Ryabykh, T. P.; Antonov, Sergei N.; Letokhov, Vladilen S.

1996-11-01

144

Assessment of Haemophilus influenzae type b opsonins by neutrophil chemiluminescence.  

PubMed Central

A luminol-enhancement chemiluminescence assay and a radiolabeled uptake assay were developed to assess opsonins for Haemophilus influenzae type b. Opsonins in acute and convalescent sera from 17 children with H. influenzae type b meningitis, along with pooled normal human sera, were evaluated and compared with anti-polyribosephosphate antibody concentrations. Five children had a rise in the chemiluminescence-area under the curve for convalescent compared with acute sera. Patient chemiluminescence--area-under-the-curve values were significantly (P less than 0.05) more likely to exceed 50% of normal human serum values if sera contained greater than or equal to 0.1 microgram of anti-polyribosephosphate antibody per ml. Magnesium ethylene glycol tetraacetic acid chelation and heat inactivation of patient and normal human sera significantly (P less than 0.05) reduced chemiluminescence--area-under-the-curve activity. Thus, complement appears to contribute significantly to the opsonization of H. influenzae type b in sera of children. Two of nine children had increases in opsonins as assayed by 3H-labeled H. influenzae type b uptake. After natural systemic H. influenzae type b infection, young children are unable to respond acutely with an increase in anti-polyribosephosphate antibody or serum opsonic activity.

Kaplan, S L; Umstead, C L; Mason, E O; Anderson, D C; Parke, J C; Feigin, R D

1981-01-01

145

Study of the H + O + M reaction forming OH{sup *}: Kinetics of OH{sup *} chemiluminescence in hydrogen combustion systems  

SciTech Connect

The temporal variation of OH{sup *} (A{sup 2}{sigma}{sup +}) chemiluminescence in hydrogen oxidation chemistry has been studied in a shock tube behind reflected shock waves at temperatures of 1400-3300 K and at a pressure of 1 bar. The aim of the present work is to obtain a validated reaction scheme to describe OH{sup *} formation in the H{sub 2}/O{sub 2} system. Temporal OH{sup *} emission profiles and ignition delay times for lean and stoichiometric H{sub 2}/O{sub 2} mixtures diluted in 97-98% argon were obtained from the shock-tube experiments. Based on a literature review for the hydrogen combustion system, the key reaction considered was H + O + M = OH{sup *} + M. The temperature dependence of the measured peak OH{sup *} emission from the shock tube and the peak OH{sup *} concentration from a homogeneous closed reactor model are compared. Based on these results a reaction rate coefficient of k{sub 1} = (1.5 {+-} 0.4) x 10{sup 13} exp(-25 kJ mol{sup -1}/RT) cm{sup 6} mol{sup -2} s{sup -1} was found for the forward reaction which is slightly higher than the rate coefficient suggested by Hidaka et al. (1982). The comparison of measured and simulated absolute concentrations shows good agreement. Additionally, a one-dimensional laminar premixed low-pressure flame calculation was performed for where absolute OH{sup *} concentration measurements have been reported by Smith et al. (2005). The absolute peak OH{sup *} concentration is fairly well reproduced if the above mentioned rate coefficient is used in the simulation. (author)

Kathrotia, T.; Riedel, U. [Institute of Combustion Technology, German Aerospace Center (DLR) and University of Stuttgart, Stuttgart (Germany); Fikri, M.; Bozkurt, M.; Hartmann, M.; Schulz, C. [IVG, University of Duisburg-Essen, Duisburg (Germany)

2010-07-15

146

Recent Advances in Chemiluminescence  

Microsoft Academic Search

This article reviews the development and application of chemiluminescence (CL) published in the literature between January 2004 and October 2006, with regard to instrumentation, systems, applications, and conclusions.

Yingying Su; He Chen; Zhimeng Wang; Yi Lv

2007-01-01

147

Saliva inhibits the chemiluminescence response, phagocytosis, and killing of Staphylococcus epidermidis by polymorphonuclear leukocytes.  

PubMed Central

Saliva inhibited several functional properties of polymorphonuclear leukocytes (PMNs) from murine peritoneal exudate, namely, luminol-mediated chemiluminescence (CL) induced by either Staphylococcus epidermidis or formylmethionyl-leucyl-phenylalanine (FMLP), phagocytosis, and killing of bacteria in vitro. The concentration of saliva in the reaction mixture that caused a complete inhibition of the CL response of PMNs to both S. epidermidis and FMLP was 25%. However, there was no catalase or superoxide dismutase activity in saliva that could influence the CL response of PMNs. The production of superoxide by PMNs stimulated with S. epidermidis was assayed in the presence or absence of saliva by inhibition of the reduction of cytochrome c by superoxide dismutase. In the presence of 50% saliva, O2- generation by PMNs was only 7.3% of that observed in the absence of saliva. After gel filtration of salivary material through Sephadex G-25 or Sephacryl S-200, several fractions were obtained that inhibited the CL response of PMNs to either FMLP or S. epidermidis or to both. Two inhibitory fractions were analyzed. One contained immunoglobulin A, and the other contained a peptide which was composed of 14 different amino acids. The two fractions of high molecular weight included in the first protein peak of Sephacryl S-200 gel filtration were able to inhibit the CL response to S. epidermidis and to inhibit phagocytic activity, while fractions of low molecular weight (under 12,500 Mr) inhibited the CL response to FMLP and to S. epidermidis but did not inhibit phagocytic activity.

Saito, K; Kato, C; Teshigawara, H

1988-01-01

148

A highly sensitive LED-induced chemiluminescence platform for aptasensing of platelet-derived growth factor.  

PubMed

In this work, we demonstrated a simple and highly sensitive LED-induced chemiluminescence (LED-CL) platform for aptasensing of proteins. It was based on the fact that fluorescein isothiocyanate (FITC) strongly catalyzed the reaction between luminol and dissolved oxygen under LED irradiation. Using FITC as a new catalytic tag, a highly sensitive sandwich type LED-CL aptasensor was fabricated for quantification of the model protein (platelet-derived growth factor-BB, PDGF-BB). Under optimized conditions, the assay for PDGF-BB was linear from 0.1 nM to 100 nM, and the detection limit was as low as 50 pM, which is better than most of other aptamer-based assays without an amplification procedure. Thrombin, bovine serum albumin, hemoglobin, cytochrome C and adenosine triphosphate showed no obvious interferences for the determination of PDGF-BB. The advantages of high sensitivity and selectivity provided by this LED-CL aptasensor will facilitate its wide application in protein assays. PMID:24179991

Zhang, Xinfeng; Zhang, Hui; Xu, Shuxia; Sun, Yonghua

2014-01-01

149

Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection.  

PubMed

A noncompetitive immunoassay based on gold nanoparticles (AuNPs) amplified capillary electrophoresis (CE) chemiluminescence (CL) detection was developed for the determination of carcinoembryonic antigen (CEA). In this method, citrate-modified AuNPs were conjugated with horseradish peroxidase (HRP) labeled CEA antibody (Ab*), and incubated with limited amount of CEA antigen. CEA-Ab*-AuNPs complex and excess of Ab*-AuNPs were then separated and quantified by CE with CL detection. Highly sensitive CL detection was achieved by means of p-iodophenol (PIP) enhanced luminol-H2O2-HPR CL reaction and AuNPs amplified. Under the optimal conditions, the CE assay was accomplished within 5min. The linear range for CEA detection was 0.05-20ng/mL with a detection limit of 0.034ng/mL (signal/noise=3), which is three orders magnitude lower than that of without AuNPs amplified. The current method was successfully applied for the quantification of CEA in human serum samples. It was demonstrated that the current CE-CL AuNPs amplified noncompetitive immunoassay was sensitive and highly selective. It may serve as a tool for clinical analysis of CEA to assist in the diagnosis of cancer. PMID:23422894

Jiang, Jing; Zhao, Shulin; Huang, Yong; Qin, Guoxin; Ye, Fanggui

2013-03-22

150

Gold nanoparticle-enhanced capillary electrophoresis-chemiluminescence assay of trace uric acid.  

PubMed

A sensitive method based on gold nanoparticle-enhanced CE-chemiluminescence (CL) detection was developed for quantifying uric acid (UA) in serum. In this work, gold nanoparticles were added into the running buffer of CE to catalyze the post-column CL reaction between luminol and hydrogen peroxide, achieving highly efficient CL emission. Negative peaks were produced due to the inhibitory effects on CL emission from UA eluted from the electrophoretic capillary. The decrease in CL intensity was proportional to the concentration of UA in the range of 2.5x10(-7)-1.0x10(-5) M. Detection limit was 4.6x10(-8) M UA. Ten human serum samples were analyzed by the presented method. Serum level of UA was found to be in the range from 204 to 324 microM for healthy subjects (n=5), and from 464 to 497 microM for diabetic patients (n=5). The two groups were significantly different (p<0.05). The results suggested a potential application of the proposed assay in rapid primary diagnosis of diseases such as diabetes. PMID:19650050

Zhao, Shulin; Lan, Xuehua; Liu, Yi-Ming

2009-08-01

151

An ultrasensitive chemiluminescent method for the tanshinol borneol ester determination and its pharmacokinetic analysis.  

PubMed

Tanshinol borneol ester (DBZ), a chemical combination of danshensu and borneol, is an experimental drug that exhibits efficacious anti-ischemic activity in animal models. In this work, an ultrasensitive chemiluminescence (CL) method for the determination of DBZ was established based on the inhibitory effect of DBZ on the CL signal produced from the reaction between potassium permanganate and luminol in alkaline solution. The CL intensity responded linearly to the concentration of DBZ in the range 2.0 × 10(-10) to 4.0 × 10(-8) g/mL with a detection limit of 7 × 10(-11) g/mL. The relative standard deviation (RSD) was 3.8% for 4.0 × 10(-9) g DBZ (n = 11). The proposed method showed characteristics of high sensitivity, simple device and quick. In addition, this proposed method had been applied satisfactorily to the analysis of DBZ in blood. The pharmacokinetics of DBZ in rat has also been studied using the CL method. PMID:23681963

Nie, Fei; Bu, Min; Wu, Lihong; Zheng, Jianbin

2014-03-01

152

MOBILE SOURCE NOX MONITOR, HYDROGEN-ATOM DIRECT CHEMILUMINESCENCE METHOD  

EPA Science Inventory

An analyzer was developed for measuring motor vehicle NOx (NO and NO2) emissions based on the chemiluminescence reaction of NO and NO2 with hydrogen atoms. This eliminated the need for an NO2 to NO converter as required with ozone chemiluminescence for NOx analysis. The hydrogen-...

153

Comparative study between chemiluminescence assay and two different sensitive polymerase chain reactions on the diagnosis of serial herpes simplex virus encephalitis  

PubMed Central

OBJECTIVE—A prospective study was undertaken on the diagnosis of herpes simplex encephalitis (HSVE) by comparing chemiluminescence assay (CL) and two different sensitive polymerase chain reactions (PCRs).?METHODS—The materials comprised 53 serial CSF samples from 31 patients with acute encephalitis with suspected HSVE. Each CSF was distributed to three independent laboratories to perform quantitative measurements by CL, the low sensitive (single) PCR, and high sensitive (nested) PCR. The CL provided a method of detecting HSV itself and the small fragment with HSV antigenicity which was composed of viral component proteins. The serial CSFs were found retrospectively to comprise 24 samples from 11 patients with HSVE due to HSV1 and 29 samples from 20 patients with non-HSVE.?RESULTS— the CL showed 50 to 48 000 pfu/ml in all samples of HSVE (except one) taken from the 3rd to the 25th day. The low sensitive PCR demonstrated 50 to 47 000 pfu/ml in only six samples of HSVE. The high sensitive PCR disclosed less than 100 to 120 000 copies/ml in 11 samples of HSVE. At the acute stage from the 1st to 7th day, the sensitivities of CL and the high sensitive PCR were 100%, but that of the low sensitive PCR was 75%. The sensitivity of CL was significantly higher than those of both PCRs after the acute stage on the 15th to 32nd day. The specificities and positive predictive values of the three methods were 100%. However, the negative predictive value of CL was significantly higher than that of the low sensitive PCR.?CONCLUSIONS—The sensitivity of CL is equivalent to that of the high sensitive PCR during the acute stage and significantly higher than that of the high sensitive PCR after the acute stage. A clear difference in sensitivity exists between the different PCRs. A combination of the PCR, chemiluminescence assay, and serological antibody diagnosis is currently considered the most effective approach for the clinical diagnosis of HSVE.??

Kamei, S; Takasu, T; Morishima, T; Yoshihara, T; Tetsuka, T

1999-01-01

154

Very low pressure reactor chemiluminescence studies on N atom reactions with CHCl sub 3 and CDCl sub 3  

Microsoft Academic Search

Ground-state (N(S⁴)) nitrogen atom reactions with chloroform-h and chloroform-d were studied by using the VLPR technique at room temperature. Relative N atom concentrations were monitored via mass spectrometry, and their absolute values were determined by the chemical titration reaction with nitric oxide. It was possible to obtain a more accurate constant for the bimolecular reaction: N + NO â Nâ

Jeoung; Choo Sae Chae; Kwang Yul; S. W. Benson

1991-01-01

155

Determination of chromium(III) and total chromium using dual channels on glass chip with chemiluminescence detection.  

PubMed

A simple, rapid and sensitive method for the determination of chromium(III) and total chromium using the simple dual T channels on glass chip with negative pressure pumping system and chemiluminescence (CL) detection is presented. The CL reaction was based on luminol oxidation by hydrogen peroxide in basic aqueous solution catalyzed by chromium(III). Total chromium in form of chromium(III) was achieved after chromium(VI) was completely reduced by acidic sodium hydrogen sulfite. Total chromium could then be determined with the same strategy as the chromium(III). The CL reagent was composed of 1.0x10(-4)mol/L luminol, 1.0x10(-2)mol/L hydrogen peroxide and 0.10mol/L sodium bromide in 0.050mol/L carbonate buffer (pH 11.00). The 1.0x10(-2)mol/L ethylenediaminetetraacetic acid was added into the sample solution in order to improve the selectivity. Chromium(III) could be detected at a notably concentration of 1.6x10(-16)mol/L and a linear calibration curve was obtained from 1.0x10(-15) to 1.0x10(-13)mol/L. The sample and CL reagent consumption were only 15 and 20microL, respectively. The analysis time was less than 1 min per sample with the precision (%R.S.D.) was 4.7%. The proposed method has been applied successfully to the analysis of river water, mineral waters, drinking waters and tap water. Its performance was verified by the analysis of certified total chromium-reference materials and by recovery measurement on spiked synthetic seawater sample. PMID:19071564

Som-Aum, Waraporn; Threeprom, Jirasak; Li, Haifang; Lin, Jin-Ming

2007-03-30

156

Chemiluminescence detector with a serpentine flow cell.  

PubMed

We present a new chemiluminescence detector, with solution channels that have been machined into a Teflon disk and sealed with a sapphire window. The configuration of the flow cell can be conveniently modified by replacing the Teflon disk. A comparison of some existing and novel designs, using the chemiluminescence reaction of morphine with acidic potassium permanganate and the bioluminescence reaction of ATP with the commercially available "BacTiter-Glo" reagent, has revealed that a serpentine channel allows greater quantities of light to be captured than a spiral channel, due to more efficient mixing of the analyte and reagent solutions within the cell. PMID:19072276

Terry, Jessica M; Adcock, Jacqui L; Olson, Don C; Wolcott, Duane K; Schwanger, Cassie; Hill, Lauren A; Barnett, Neil W; Francis, Paul S

2008-12-15

157

Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schro?dinger equation.  

PubMed

Direct computation of energies and two-electron reduced density matrices (2-RDMs) from the anti-Hermitian contracted Schro?dinger equation (ACSE) [D. A. Mazziotti, Phys. Rev. Lett. 97, 143002 (2006)], it is shown, recovers both single- and multi-reference electron correlation in the chemiluminescent reaction of dioxetanone especially in the vicinity of the conical intersection where strong correlation is important. Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical intersection. Our previous active-space 2-RDM study of dioxetanone [L. Greenman and D. A. Mazziotti, J. Chem. Phys. 133, 164110 (2010)] concluded that correlating 16 electrons in 13 (active) orbitals is required for realistic surfaces without correlating the remaining (inactive) orbitals. In this paper we pursue two complementary goals: (i) to correlate the inactive orbitals in 2-RDMs along dioxetanone's reaction coordinate and compare these results with those from multireference second-order perturbation theory (MRPT2) and (ii) to assess the size of the active space-the number of correlated electrons and orbitals-required by both MRPT2 and ACSE for accurate energies and surfaces. While MRPT2 recovers very different amounts of correlation with (4,4) and (16,13) active spaces, the ACSE obtains a similar amount of correlation energy with either active space. Nevertheless, subtle differences in excitation energies near the conical intersection suggest that the (16,13) active space is necessary to determine both energetic details and properties. Strong electron correlation is further assessed through several RDM-based metrics including (i) total and relative energies, (ii) the von Neumann entropy based on the 1-electron RDM, as well as the (iii) infinity and (iv) squared Frobenius norms based on the cumulant 2-RDM. PMID:21548676

Greenman, Loren; Mazziotti, David A

2011-05-01

158

Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schroedinger equation  

SciTech Connect

Direct computation of energies and two-electron reduced density matrices (2-RDMs) from the anti-Hermitian contracted Schroedinger equation (ACSE) [D. A. Mazziotti, Phys. Rev. Lett. 97, 143002 (2006)], it is shown, recovers both single- and multi-reference electron correlation in the chemiluminescent reaction of dioxetanone especially in the vicinity of the conical intersection where strong correlation is important. Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical intersection. Our previous active-space 2-RDM study of dioxetanone [L. Greenman and D. A. Mazziotti, J. Chem. Phys. 133, 164110 (2010)] concluded that correlating 16 electrons in 13 (active) orbitals is required for realistic surfaces without correlating the remaining (inactive) orbitals. In this paper we pursue two complementary goals: (i) to correlate the inactive orbitals in 2-RDMs along dioxetanone's reaction coordinate and compare these results with those from multireference second-order perturbation theory (MRPT2) and (ii) to assess the size of the active space--the number of correlated electrons and orbitals--required by both MRPT2 and ACSE for accurate energies and surfaces. While MRPT2 recovers very different amounts of correlation with (4,4) and (16,13) active spaces, the ACSE obtains a similar amount of correlation energy with either active space. Nevertheless, subtle differences in excitation energies near the conical intersection suggest that the (16,13) active space is necessary to determine both energetic details and properties. Strong electron correlation is further assessed through several RDM-based metrics including (i) total and relative energies, (ii) the von Neumann entropy based on the 1-electron RDM, as well as the (iii) infinity and (iv) squared Frobenius norms based on the cumulant 2-RDM.

Greenman, Loren; Mazziotti, David A. [Department of Chemistry and James Franck Institute, University of Chicago, Chicago, Illinois 60637 (United States)

2011-05-07

159

Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schrödinger equation  

NASA Astrophysics Data System (ADS)

Direct computation of energies and two-electron reduced density matrices (2-RDMs) from the anti-Hermitian contracted Schrödinger equation (ACSE) [D. A. Mazziotti, Phys. Rev. Lett. 97, 143002 (2006)], it is shown, recovers both single- and multi-reference electron correlation in the chemiluminescent reaction of dioxetanone especially in the vicinity of the conical intersection where strong correlation is important. Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical intersection. Our previous active-space 2-RDM study of dioxetanone [L. Greenman and D. A. Mazziotti, J. Chem. Phys. 133, 164110 (2010)] concluded that correlating 16 electrons in 13 (active) orbitals is required for realistic surfaces without correlating the remaining (inactive) orbitals. In this paper we pursue two complementary goals: (i) to correlate the inactive orbitals in 2-RDMs along dioxetanone's reaction coordinate and compare these results with those from multireference second-order perturbation theory (MRPT2) and (ii) to assess the size of the active space--the number of correlated electrons and orbitals--required by both MRPT2 and ACSE for accurate energies and surfaces. While MRPT2 recovers very different amounts of correlation with (4,4) and (16,13) active spaces, the ACSE obtains a similar amount of correlation energy with either active space. Nevertheless, subtle differences in excitation energies near the conical intersection suggest that the (16,13) active space is necessary to determine both energetic details and properties. Strong electron correlation is further assessed through several RDM-based metrics including (i) total and relative energies, (ii) the von Neumann entropy based on the 1-electron RDM, as well as the (iii) infinity and (iv) squared Frobenius norms based on the cumulant 2-RDM.

Greenman, Loren; Mazziotti, David A.

2011-05-01

160

Inhibition of chemiluminescence and chemotactic activity of phagocytes in vitro by the extracts of selected medicinal plants.  

PubMed

The methanol extracts of 20 selected medicinal plants were investigated for their effects on the respiratory burst of human whole blood, isolated human polymorphonuclear leukocytes (PMNs) and isolated mice macrophages using a luminol/lucigenin-based chemiluminescence assay. We also tested the effect of the extracts on chemotactic migration of PMNs using the Boyden chamber technique. The extracts of Curcuma domestica L., Phyllanthus amarus Schum & Thonn and C. xanthorrhiza Roxb. were the samples producing the strongest oxidative burst of PMNs with luminol-based chemiluminescence, with IC(50) values ranging from 0.5 to 0.7 ?g/ml. For macrophage cells, the extracts which showed strong suppressive activity for luminol-based chemiluminescence were C. xanthorrhiza and Garcinia mangostana L. Among the extracts studied, C. mangga Valton & Vazsjip, Piper nigrum L. and Labisia pumila var. alata showed strong inhibitory activity on lucigenin-amplified oxidative burst of PMNs, with IC(50) values ranging from 0.9 to 1.5 ?g/ml. The extracts of Zingiber officinale Rosc., Alpinia galangal (L.) Willd and Averrhoa bilimbi Linn showed strong inhibition on the chemotaxic migration of cells, with IC(50) values comparable to that of ibuprofen (1.5 ?g/ml). The results suggest that some of these plants were able to modulate the innate immune response of phagocytes at different steps, emphasizing their potential as a source of new immunomodulatory agents. PMID:21184195

Jantan, Ibrahim; Harun, Nurul Hikmah; Septama, Abdi Wira; Murad, Shahnaz; Mesaik, M A

2011-04-01

161

Chemiluminescence involving acidic and ambient ion light emitters. The chemiluminescence of the 9-acridinepercarboxylate anion  

SciTech Connect

The reaction of phenyl 9-acridinecarboxylate with an excess of peroxide ion in THF/water (67/33 mol %) leads to the emission of either bright yellow-green light or bright blue light, depending on the reaction conditions. The blue emission is favored by high concentrations of hydrogen peroxide and water, for example. 9-Acridinepercarboxylic acid is a common intermediate in the reactions. The light emitter responsible for the blue chemiluminescence is acridone, whereas that responsible for the yellow-green chemiluminescence is the anion of acridone. The effects of base concentration and solvent composition on the relative proportions of these two emitters have produced evidence that, contrary to the expectation of simple theory, a dioxetanone is not an intermediate in the reaction. Other cases where chemiluminescence may involve percarboxylate and peroxide ions are discussed.

White, E.H.; Roswell, D.F.; Dupont, A.C.; Wilson, A.A.

1987-08-19

162

A novel HPLC-UV/nano-TiO2-chemiluminescence system for the determination of selenocystine and selenomethionine.  

PubMed

Active oxygen species from the photocatalytic reaction in aqueous solution react with luminol to emit strong chemiluminescence (CL), and this can be inhibited by the UV decomposed-products of selenocystine (SeCys) or selenomethionine (SeMet). Based on this phenomenon, a novel hyphenated technique, HPLC-UV/nano-TiO(2)-CL, was established for the determination of SeCys and SeMet. The effects of pH, the UV irradiation time, the TiO(2) coated on the inner surface of the reaction tubing, and the Co(2+) catalyst concentration on the CL intensity and/or chromatographic resolution were systematically investigated. Under these optimized conditions, the inhibited CL intensity has a good linear relationship with the concentration of SeCys in the range of 0.04-10.6 microg mL(-1) or SeMet in the range of 0.05-12.4 microg mL(-1), with a limit of detection (S/N=3) of 6.4 microg L(-1) for SeCys or 12 microg L(-1) for SeMet. As an example, the method was preliminarily applied to the determination of the selenoamino acids in garlic and rabbit serum, with a recovery of 88-104%. PMID:18585988

Su, Yingying; Chen, He; Gao, Ying; Li, Xiaohong; Hou, Xiandeng; Lv, Yi

2008-07-15

163

Electrochemiluminescence of luminol on a platinum-nanoparticle-modified indium tin oxide electrode in neutral aqueous solution.  

PubMed

Electrochemiluminescence (ECL) behavior of luminol on an indium tin oxide (ITO) electrode modified with platinum nanoparticles (PtNPs) was investigated in a neutral aqueous solution using the conventional cyclic voltammetry (CV) technique. Experimental results indicated that the ECL behaviors of luminol on the PtNPs modified electrode showed significant difference from those on the bare ITO or bulk platinum electrodes. Five ECL peaks were found at 0.60, 0.92, 0.70, -0.44 and -1.16 V versus a saturated calomel electrode (SCE), respectively. The ECL peaks were found to depend on the reaction medium conditions including the type of electrolyte, pH value, the presence or absence of O2 and the different kinds of nanoparticles, as well as the scan direction and range of the applied potential. Furthermore, ECL peaks at -0.44 and -1.16 V could only be obtained on the PtNPs/ITO electrode. The surface state of the electrode was characterized by ultraviolet-visible (UV-Vis) absorption, scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). A mechanism for luminol ECL on the PtNPs/ITO electrode was proposed. The excellent ECL properties of luminol on the PtNPs/ITO electrode in the neutral medium revealed a great potential for analytical applications to biological samples. PMID:19437984

Chen, Xiaomei; Lin, Zhijie; Cai, Zhiming; Chen, Xi; Oyama, Munetaka; Wang, Xiaoru

2009-04-01

164

Biological water quality monitoring using chemiluminescent and bioluminescent techniques  

NASA Technical Reports Server (NTRS)

Automated chemiluminescence and bioluminescence sensors were developed for the continuous monitoring of microbial levels in water supplies. The optimal chemical procedures were determined for the chemiluminescence system to achieve maximum sensitivity. By using hydrogen peroxide, reaction rate differentiation, ethylene diamine tetraacetic acid (EDTA), and carbon monoxide pretreatments, factors which cause interference were eliminated and specificity of the reaction for living and dead bacteria was greatly increased. By employing existing technology with some modifications, a sensitive and specific bioluminescent system was developed.

Thomas, R. R.

1978-01-01

165

Chemi-luminescence measurements of hyperthermal Xe{sup +}/Xe{sup 2+}+ NH{sub 3} reactions  

SciTech Connect

Luminescence spectra are recorded for the reactions of Xe{sup +}+ NH{sub 3} and Xe{sup 2+}+ NH{sub 3} at energies ranging from 11.5 to 206 eV in the center-of-mass (E{sub cm}) frame. Intense features of the luminescence spectra are attributed to the NH (A {sup 3}{Pi}{sub i}-X {sup 3}{Sigma}{sup -}), hydrogen Balmer series, and Xe I emission observable for both primary ions. Evidence for charge transfer products is only found through Xe I emission for both primary ions and NH{sup +} emission for Xe{sup 2+} primary ions. For both primary ions, the absolute NH (A-X) cross section increases with collision energy before leveling off at a constant value, approximately 9 x 10{sup -18} cm{sup 2}, at about 50 eV while H-{alpha} emission increases linearly with collision energy. The nascent NH (A) populations derived from the spectral analysis are found to be independent of collision energy and have a constant rotational temperature of 4200 K.

Prince, Benjamin D.; Steiner, Colby P. [Air Force Research Laboratory, Space Vehicles Directorate, Kirtland AFB, New Mexico 87117 (United States); Chiu, Yu-Hui [Busek Co. Inc, Natick, Massachusetts 01760 (United States)

2012-04-14

166

Luminol-based bioluminescence imaging of mouse mammary tumors.  

PubMed

Polymorphonuclear neutrophils (PMNs) are the most abundant circulating blood leukocytes. They are part of the innate immune system and provide a first line of defense by migrating toward areas of inflammation in response to chemical signals released from the site. Some solid tumors, such as breast cancer, also cause recruitment and activation of PMNs and release of myeloperoxidase. In this study, we demonstrate that administration of luminol to mice that have been transplanted with 4T1 mammary tumor cells permits the detection of myeloperoxidase activity, and consequently, the location of the tumor. Luminol allowed detection of activated PMNs only two days after cancer cell transplantation, even though tumors were not yet palpable. In conclusion, luminol-bioluminescence imaging (BLI) can provide a pathway towards detection of solid tumors at an early stage in preclinical tumor models. PMID:24077442

Alshetaiwi, Hamad S; Balivada, Sivasai; Shrestha, Tej B; Pyle, Marla; Basel, Matthew T; Bossmann, Stefan H; Troyer, Deryl L

2013-10-01

167

A High-Performance Liquid Chromatography:Chemiluminescence Method for Potential Determination of Vardenafil in Dietary Supplement  

PubMed Central

A flow method of high-performance liquid chromatography (HPLC) seperation and chemiluminescence (CL) detection for sensitive vardenafil analysis in dietary supplements was developed. The vardenafil separation was achieved on a C18 column at 30°C using ethanol-H3PO4 and ethylenediaminetetraacetic acid disodium salt (Na2EDTA) aqueous solution (25?:?75, v/v%) as mobile phase. The followed continuous CL detection was conducted based on the strong CL enhancement by the presence of vardenafil to luminol-K3Fe(CN)6 reaction in alkaline medium. At the flow rate of 0.8?mL/min, the vardenafil retention time (tR) was 6.4 min. Factors that affected the HPLC resolution and CL detection were studied and optimized. The calibration curve obtained for vardenafil standard was linear in concentration range of 8.0 × 10?7 ~ 1.0 × 10?4?mol/L. The relative standard deviations (RSD) of intraday and interday precision were less than 3.5%. The proposed method was applied to the vardenafil determination in oral liquid, wine, and capsule samples.

Di, Youjun; Zhao, Min; Nie, Yingchun; Wang, Fei; Lv, Jiagen

2011-01-01

168

Antioxidant activity of bisabolol: inhibitory effects on chemiluminescence of human neutrophil bursts and cell-free systems.  

PubMed

Human polymorphonuclear neutrophils (PMN), reactive oxygen species (ROS) and inflammatory reactions are closely interrelated, and increasing attention is being given to the search for new synthetic or natural antioxidant agents, capable of reducing ROS and consequent inflammation. It has been claimed that bisabolol (a monocyclic sesquiterpene alcohol) has an antioxidant/anti-inflammatory activity, but this has almost exclusively been investigated using chemical or biochemical tests. We studied the ability of bisabolol to interfere with ROS production (luminol-amplified chemiluminescence, LACL) during human PMN respiratory bursts induced by both corpusculate(Candida albicans)and soluble stimulants (N-formyl-methionyl-leucyl-phenylalanine, fMLP). LACL was also used to test cell-free systems (SIN-1 and H2O2/HOCl(-) systems) in order to investigate the presence of scavenging activity. After C. albicans stimulation, significant concentration-dependent LACL inhibition was observed at bisabolol concentrations ranging from 7.7 to 31 microg/ml; after the fMLP stimulus, significant LACL inhibition was observed at bisabolol concentrations ranging from 3.8 to 31 microg/ml. A similar effect was observed in the SIN-1 and H2O2/HOCl(-) systems. These findings draw the attention to the possible medical use of bisabolol as a means of improving the antioxidant network and restoring the redox balance by antagonising oxidative stress. PMID:19096233

Braga, Pier Carlo; Dal Sasso, Monica; Fonti, Elena; Culici, Maria

2009-01-01

169

Concerning Chemiluminescence of Rhodamine C.  

National Technical Information Service (NTIS)

A chemiluminescence method of measuring ozone in the atmosphere is described. Methods of preparing chemiluminescent materials using Reginer's technique involving Rhodamine C are discussed, as well as the luminescence spectrum of this material and results ...

L. G. Bolshakova V. A. Vasiliyeva

1972-01-01

170

In situ detection of diamine oxidase activity using enhanced chemiluminescence.  

PubMed

In need of a simple and sensitive method for detection of diamine oxidase (EC 1.4.3.6) activity in connection with diamine oxidase purification from human placenta, we have developed an enhanced chemiluminescence method using putrescine as substrate and horseradish peroxidase and luminol for the detection of the H2O2 produced by diamine oxidase. The method allows direct detection of small amounts of diamine oxidase in serum samples after agarose gel electrophoresis and allows visualization of diamine oxidase activity in tissue sections. Employing this method we have detected diamine oxidase in sera from cow, horse, monkey, rabbit, and pregnant women. On tissue sections from term human placenta diamine oxidase activity was exclusively localized to the maternal side and was concentrated in vessels and fibrinoid areas. PMID:8789157

Bruun, L; Houen, G

1996-01-01

171

A simple chemiluminescence assay for the determination of reactive oxygen species produced by human neutrophils.  

PubMed

We show that phagocyte production of reactive oxygen species can be measured using a microtitre plate based chemiluminescence blotting technique. The production of reactive oxygen species is determined by their ability to catalyze the oxidation of luminol or isoluminol, resulting in light emission which is recorded on a photographic film. The method permits the determination of NADPH oxidase activity from as few as 9000 cells. It could be used to detect NADPH oxidase defects in neutrophils (e.g. from patients suffering from chronic granulomatous disease), and to screen pharmaceuticals with scavenging activity for reactive oxygen species. PMID:8699014

Liu, L; Dahlgren, C; Elwing, H; Lundqvist, H

1996-06-10

172

Microscale on-line production and determination of malic acid using flow injection analysis, immobilized fumrase and malate dehydrogenase with chemiluminescence detection  

Microsoft Academic Search

Microscale on-line production of l-malic acid from fumaric acid using on-line immobilized fumrase is developed. Fumaric acid is aspirated continuously through immobilized fumarase using a flow injection manifold. A simple and rapid flow injection chemiluminescence system based on an immobilized malate dehydrogenase, methylene blue and luminol system is developed to monitor the production of malic acid. The log-log calibration graph

Ala’ddin M Almuaibed

2001-01-01

173

Chemiluminescent screening of quenching effects of natural colorants against reactive oxygen species: Evaluation of grape seed, monascus, gardenia and red radish extracts as multi-functional food additives  

Microsoft Academic Search

The quenching effects of commercial available natural colorants against reactive oxygen species (ROS) were investigated. The effectiveness was evaluated by measuring the quenching ratio of chemiluminescence (CL) intensity with each colorant on luminol or methyl-6-(p-methoxyphenyl)-3,7-dihydroimidaz[1,2-a]pyrazin-3-one CL induced by ROS. As a result, the grape seed extracts and polyphenolics dose-dependently quenched CL. The quenching effects of grape seed extract A at

M. Wada; H. Kido; K. Ohyama; T. Ichibangase; N. Kishikawa; Y. Ohba; M. N. Nakashima; N. Kuroda; K. Nakashima

2007-01-01

174

A Study of Prevention of Spontaneous Luminescence of Luminol.  

National Technical Information Service (NTIS)

Two methods are conceivable in preventing the spontaneous luminescence of a luminol reagent. One is to reduce the alkalinity of sodium carbonate by adding some reagents of weak acidity. The other method is to add reagents that suppress the decomposition o...

M. Arai

1964-01-01

175

Theoretical study of the correlation between superoxide anion consumption and firefly luciferin chemiluminescence  

NASA Astrophysics Data System (ADS)

This is the first theoretical study of the relationship between superoxide anion and firefly chemiluminescence, in DMSO. Electron transfer reactions between luciferin dianionic/carbanionic/radical species and superoxide were studied in order see if an alternative explanation existed for the consumption of the latter species, without correlating it with a role on luciferin chemiluminescence. Despite the finding that luciferin may indeed inhibit the formation of the superoxide anion, no theoretical evidence was found that showed that this molecule is consumed in a non-chemiluminescence reaction. Therefore, it is concluded that the superoxide anion is indeed related to the firefly luciferin chemiluminescence.

Pinto da Silva, Luís; Esteves da Silva, Joaquim C. G.

2013-07-01

176

Label-free chemiluminescent aptasensor for platelet-derived growth factor detection based on exonuclease-assisted cascade autocatalytic recycling amplification.  

PubMed

Here an exonuclease III (Exo III)-assisted cascade autocatalytic recycling amplification (Exo-CARA) strategy is proposed for label-free chemiluminescent (CL) detection of platelet-derived growth factor BB (PDGF-BB) by taking advantage of both recognition property of aptamer and cleavage function of Exo III. Functionally, this system consists of a duplex DNA (aptamer-blocker hybrid), two kinds of hairpin structures (MB1 and MB2), and Exo III. Upon recognizing and binding with PDGF-BB, aptamer folds into a close configuration, which initiates the proposed Exo-CARA reaction (Recyclings I?II?III?II). Finally, numerous "caged" G-quadruplex sequences on DNAzyme1 and DNAzyme2 release that intercalate hemin to catalyze the oxidation of luminol by H2O2 to generate an amplified CL signal, achieving excellent specificity and high sensitivity with a detection limit of 6.8×10(-13)M PDGF-BB. The proposed strategy has the advantages of simple design, isothermal conditions, homogeneous reaction without separation and washing steps, effective-cost without the need of labeling, and high amplification efficiency, which might be a universal and promising protocol for the detection of a variety of biomolecules whose aptamers undergo similar conformational changes. PMID:25016251

Bi, Sai; Luo, Baoyu; Ye, Jiayan; Wang, Zonghua

2014-12-15

177

Sensitive competitive flow injection chemiluminescence immunoassay for IgG using gold nanoparticle as label  

NASA Astrophysics Data System (ADS)

A sensitive competitive flow injection chemiluminescence (CL-FIA) immunoassay for immunoglobulin G (IgG) was developed using gold nanoparticle as CL label. In the configuration, anti-IgG antibody was immobilized on a glass capillary column surface by 3-(aminopropyl)-triethoxysilane and glutaraldehyde to form immunoaffinity column. Analyte IgG and gold nanoparticle labeled IgG were passed through the immunoaffinity column mounted in a flow system and competed for the surface-confined anti-IgG antibody. CL emission was generated from the reaction between luminol and hydrogen peroxide in the presence of Au (III), generated from chemically oxidative dissolution of gold nanoparticle by an injection of 0.10 mol L -1 HCl-0.10 mol L -1 NaCl solution containing 0.10 mmol L -1 Br 2. The concentration of analyte IgG was inversely related to the amount of bound gold nanoparticle labeled IgG and the CL intensity was linear with the concentration of analyte IgG from 1.0 ng mL -1 to 40 ng mL -1 with a detection limit of 5.2 × 10 -10 g mL -1. The whole assay time including the injections and washing steps was only 30 min for one sample, which was competitive with CL immunoassays based on a gold nanoparticle label and magnetic separation. This work demonstrates that the CL immunoassay incorporation of nanoparticle label and flow injection is promising for clinical assay with sensitivity and high-speed.

Qi, Honglan; Shangguan, Li; Liang, Lin; Ling, Chen; Gao, Qiang; Zhang, Chengxiao

2011-11-01

178

Micro flow sensor on a chip for the determination of terbutaline in human serum based on chemiluminescence and a molecularly imprinted polymer.  

PubMed

Based on a molecularly imprinted polymer (MIP) as the recognition element, a novel chemiluminescence (CL) micro flow sensor on a chip for the determination of terbutaline in human serum is described. The MIP was prepared by using terbutaline as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linking monomer, and acetonitrile as the solvent. The chip was fabricated from two 50 mm x 40 mm x 5 mm transparent poly (methylmethacrylate) (PMMA) slices. The microchannels on the chip etched by CO(2) laser were 200 microm wide and 150 microm deep. The microsensor cell filled with 2mg MIP for selectively on line adsorbing terbutaline was 10 mm long, 1 mm wide, and 0.5 mm deep. All reagents were controlled by the syringe pump with an accurate timer. The on line adsorbed terbutaline by the MIP can enhance the CL intensity of the reaction of luminol with ferricyanide. The enhanced CL intensity is linear with terbutaline concentration from 8.0 to 100 ng/mL with a detection limit of 4.0 ng/mL (3sigma). The micro flow sensor provides for good reproducibility with the relative standard deviation of 3.6% (n=7) for 20 ng/mL terbutaline. PMID:18970705

He, Deyong; Zhang, Zhujun; Zhou, Houjiang; Huang, Ying

2006-07-15

179

Chemiluminescence immunoassay for the rapid and sensitive detection of antibody against porcine parvovirus by using horseradish peroxidase/detection antibody-coated gold nanoparticles as nanoprobes.  

PubMed

A rapid, simple, facile, sensitive and enzyme-amplified chemiluminescence immunoassay (CLIA) method to detect antibodies against porcine parvovirus has been developed. Horseradish peroxidase (HRP) and the detection antibody were simultaneously co-immobilized on the surface of gold nanoparticles using the electrostatic method to form gold nanoparticle-based nanoprobes. This nanoprobe was employed in a sandwich-type CLIA, which enables CL signal readout from enzymatic catalysis and results in signal amplification. The presence of porcine parvovirus infection was determined in porcine parvovirus antibodies by measuring the CL intensity caused by the reaction of HRP-luminol with H2 O2 . Under optimal conditions, the obtained calibration plot for the standard positive serum was approximately linear within the dilution range of 1:80 to 1:5120. The limit of detection for the assay was 1:10,240 (S/N?=?3), which is much lower than that typically achieved with an enzyme-linked immunosorbent assay (1:160; S/N?=?3). A series of repeatability measurements using 1:320-fold diluted standard positive serum gave reproducible results with a relative standard deviation of 4.9% (n?=?11). The ability of the immunosensor to analyze clinical samples was tested on porcine sera. The immunosensor had an efficiency of 90%, a sensitivity of 93.3%, and a specificity of 87.5% relative to the enzyme-linked immunosorbent assay results. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23832716

Zhou, Yuan; Zhou, Tao; Zhou, Rui; Hu, Yonggang

2014-06-01

180

Real-time monitoring of picomolar concentrations of iron(II) in marine waters using automated flow injection-chemiluminescence instrumentation.  

PubMed

A shipboard-deployable, flow-injection (FI) based instrument for monitoring iron(II) in surface marine waters is described. It incorporates a miniature, low-power photon-counting head for measuring the light emitted from the iron(II)-catalyzed chemiluminescence (CL) luminol reaction. System control, signal acquisition, and data processing are performed in a graphical programming environment. The limit of detection for iron(II) is in the range 8-12 pmol L(-1) (based on 3 s of the blank), and the precision over the range 8-1000 pmol L(-1) varies between 0.9 and 7.6% (n = 4). Results from a day-night deployment during a north-to-south transect of the Atlantic Ocean and a daytime transect in the Sub-Antarctic Front are presented together with ancillary temperature, salinity, and irradiance data. The generic nature of the components used to assemble the instrument make the technology readily transferable to other laboratories and the modular construction makes it easy to adapt the system for use with other CL chemistries. PMID:12433170

Bowie, Andrew R; Achterberg, Eric P; Sedwick, Peter N; Ussher, Simon; Worsfold, Paul J

2002-11-01

181

Determination of oxides of nitrogen (NO\\/sub x\\/) in cigarette smoke by chemiluminescent analysis  

Microsoft Academic Search

The successful application of a commercial chemiluminescent No\\/sub x\\/ analyzer to the determination of oxides of nitrogen in cigarette smoke is reported. Individual puffs of the smoke vapor phase are rapidly diluted in an air stream before introduction into the analyzer. This acts to both reduce quenching of the chemiluminescent response by COâ and to prevent side reactions of the

R. A. Jenkins; B. E. Gill

1980-01-01

182

Crossed-Beam Chemiluminescence Studies of Some Group IIa Metal Oxides.  

National Technical Information Service (NTIS)

Chemiluminescence from a beam of Group IIa metals traversing a scattering chamber filled with NO2 or N2O gas has been studied as a function of wavelength and pressure. The Ba + NO2 and the Ba, Sr, and Ca + N2O chemiluminescent reactions are first order in...

C. D. Jonah R. N. Zare C. Ottinger

1971-01-01

183

Modulatory effect of visible light on chemiluminescence of stimulated and nonstimulated blood leukocytes of carp (Cyprinus carpio, L)  

NASA Astrophysics Data System (ADS)

Irradiation of carp blood leukocytes with a non-laser visible light resulted in a significant inhibition of the spontaneous luminol-dependent chemiluminescence in the cells of a part of the fish. Those leukocytes that were sensitive to the visible light, showed a shorter time-to-peak than the non sensitive, following their stimulation with Ca ionophore. Because a shorter time-to-peak correlates with inflammation, it could be suggested that the visible light susceptible leukocyte reflect a pre-inflammatory state of their donors.

Belotsky, Sandro; Avtalion, Ramy R.; Friedmann, Harry; Lubart, Rachel

1998-12-01

184

Use of chemiluminescence techniques in portable, lightweight, highly sensitive instruments for measuring NO2, NO(x), and O3  

NASA Astrophysics Data System (ADS)

Small battery-powered instruments for the sensitive detection of nitrogen dioxide (NO2), and ozone (O3) are described. Because of their portability, they are ideal for studies of indoor air quality, measurements from aircraft, and other mobile platforms where power may be limited. For example, a miniature balloon-borne sonde, based on the chemiluminescence between luminol and NO2 has recently demonstrated the ability to measure ambient mixing ratios of NO2 between ground level and an altitude of 33 km. Recent 'add-on's to the model LMA-3 NO2 analyzer are discussed and the model LOZ-4 ozone monitor is described.

Drummond, J. W.; Topham, L. A.; Mackay, G. I.; Schiff, H. I.

1991-05-01

185

Phagocytic and chemiluminescent responses of mouse peritoneal macrophages to living and killed Salmonella typhimurium and other bacteria  

SciTech Connect

In the presence of luminol, resident as well as thioglycolate-induced and immunized macrophages emitted chemiluminescence more efficiently when the cells were exposed to living Salmonella typhimurium than when they were exposed to the same bacterium killed by ultraviolet light or heat. This phenomenon was observed whether or not the bacterium was opsonized. The different response to living and killed bacteria was also found with Escherichia coli, Pseudomonas aeruginosa, Proteus morganii, and Enterobacter aerogenes, but not with Shigella sonnei, Klebsiella pneumoniae, and Propionibacterium acnes. The results suggest that macrophages respond better to living, motile bacteria than to nonmotile or killed bacteria. The experimental results obtained with motility mutants of S. typhimurium, E. coli, and P. aeruginosa confirm that macrophages exposed to the motile bacteria emit chemiluminescence more efficiently and ingest the motile bacteria at a much faster rate than the nonmotile bacteria.

Tomita, T.; Blumenstock, E.; Kanegasaki, S.

1981-06-01

186

A rapid chemiluminescent enzyme-linked immunosorbent assay for cytomegalovirus immunoglobulin G antibodies using instant photographic film.  

PubMed

A rapid and convenient chemiluminescent enzyme-linked immunosorbent assay (ELISA) for IgG antibodies to cytomegalovirus has been developed which uses low cost equipment. Assays were carried out on transparent microtitre plates and used an anti-human IgG horseradish peroxidase conjugate. Bound peroxidase was detected chemiluminescently using a p-iodophenol-luminol-peroxide reagent. Light emission from the wells of the microtitre plate was detected on instant photographic film (ASA 20,000) held in a specially designed shutter type camera. The semi-quantitative technique was tested in a routine laboratory for a period of 7 wk and the results obtained compared well (95.3% agreement) with those obtained by a conventional colorimetric ELISA using an alkaline phosphatase label. PMID:3009516

Nickless, G G; Thorpe, G H; Kricka, L J; Whitehead, T P; Wells, L J; Ala, F A

1985-12-01

187

Chemiluminescent detection of reduced sulfur compounds with ozone  

SciTech Connect

With the aim of developing a simple, sensitive, selective real-time method for measurement of reduced sulfur gases, we have investigated the chemiluminescent detection of H/sub 2/S, DMS, and other reduced sulfur compounds by their oxidation with ozone. The observation of chemiluminescence in the 300 to 400 nm wavelength region resulting from the gas-phase reaction of O/sub 3/ with H/sub 2/S, DMS, and methyl mercaptan (C-H/sub 3/SH) has been identified as electronically excited SO/sub 2/. The homogeneous gas-phase reaction of ozone with hydrogen sulfide has been reported to be slow in studies at low pressures and the oxidation mechanism is apparently complex. A reaction order for H/sub 2/S from 0.5 to 2 has been observed, and a heterogeneous reaction pathway has been proposed as a possible explanation of the data. Reported here are results of a preliminary investigation of the ozone-reduced sulfur chemiluminescence, which also imply a complex reaction mechanism (possibly both homogeneous and heterogeneous) but indicate that ozone chemiluminescence may be an extremely useful tool for the detection of H/sub 2/S, DMS, and other reduced sulfur species in the ambient atmosphere and in industrial applications.

Kelly, T.J.; Gaffney, J.S.; Phillips, M.F.; Tanner, R.L.

1983-01-01

188

Enzymatic chemiluminescent assay of glucose by sequential-injection analysis with soluble enzyme and on-line sample dilution.  

PubMed

This work reports a sequential-injection analysis (SIA) method for the enzymatic assay of glucose with soluble glucose oxidase (GOD) and on-line sample dilution with chemiluminescence (CL) detection. A zone of sample was aspirated in the holding coil of the SIA manifold and, if necessary, was diluted on-line by means of an auxiliary dilution conduit. Then, a zone of GOD was aspirated adjacent to the sample zone and a stopped-flow period was applied to allow the enzymatic reaction to proceed with production of hydrogen peroxide. Then, zones of a catalyst (Co(II) solution) and alkaline luminol were aspirated into the holding coil. Finally, the flow was reversed and the stacked zones were sent to a flow-cell located in front of a photomultiplier tube (PMT) that monitored the CL intensity. The linear dynamic range was 1 x 10(-5)-1 x 10(-3) mol L(-1) glucose, the coefficient of variation at 8 x 10(-5) mol L(-1) of glucose was s(r)=3.1% (n=8), the limit of detection at the 3sigma level was c(L)= 1 x 10(-6) mol L(-1) and the sampling frequency was 28 h(-1). With on-line dilution by a factor of 1/200, the linear range could be extended up to 0.2 mol L(-1) glucose. The advantages of the proposed method are the simple manifold and instrumentation used, the scope for automated on-line dilution, the low consumption of sample and reagents and the elimination of enzyme immobilisation procedures. The method was applied to the analysis of commercial drinks and honey with percent relative errors in glucose determination in the range 100+/-6.1%. PMID:17723471

Economou, Anastasios; Panoutsou, Polyxeni; Themelis, Demetrius G

2006-07-14

189

Chemiluminescence switching on peroxidase-like Fe3O4 nanoparticles for selective detection and simultaneous determination of various pesticides.  

PubMed

To achieve selectivity in direct chemiluminescence (CL) detection is very significant and a great challenge as well. Here, we report a novel concept of developing intrinsically selective CL switching at the surface of Fe(3)O(4) nanoparticles for the sensitive detection and simultaneous determination of various pesticides. Fe(3)O(4) nanoparticles have peroxidase-like catalytic activity and catalyze the decomposition of dissolved oxygen to generate superoxide anions, so that the CL intensity of luminol was amplified by at least 20 times. The CL signals can be quenched by the addition of ethanol because ethanol readily reacts with superoxide anions as a radical scavenger. However, the quenching effect can be inhibited through the specific binding of target molecules on Fe(3)O(4) nanoparticles, leading to CL "turn-on" in the presence of ethanol. The novel CL "switching-on" concept demonstrated unique advantages in the detection of pesticide residues. Using the surface coordinative reactions, nonredox pesticide ethoprophos were sensitively detected with a detection limit of 0.1 nM and had a very wide detection range of 0.1 nM to 100 ?M. More importantly, the selectivity of CL switching is tunable through the special surface modification of Fe(3)O(4) nanoparticles, and these Fe(3)O(4) nanoparticles with different surface groups can generate unique CL response pattern for the simultaneous determination of various pesticides. Meanwhile, the superparamagnetic properties of Fe(3)O(4) nanoparticles provide a simple magnetic separation approach to attain interference-free measurement for real detection. The very facile and versatile strategy reported here should open a new window to exploration of selective CL molecular switching and application of magnetic nanoparticles for chemo/biodetection. PMID:23025448

Guan, Guijian; Yang, Liang; Mei, Qingsong; Zhang, Kui; Zhang, Zhongping; Han, Ming-Yong

2012-11-01

190

The effect of storage on whole blood chemiluminescence measurement of equine neutrophils.  

PubMed

The aim of this study was to determine the effect of duration and temperature of sample storage on whole blood chemiluminescence measurement results. Venous blood from 18 clinically healthy Polish half-bred horses aged 4 to 11 years were used in the study. Luminol dependent chemiluminescence (CL) was used to measure neutrophil oxygen metabolism in whole blood. Blood samples were examined for spontaneous CL and stimulated by a surface receptor stimulus as well as extra-receptor stimulus. The assay was performed in two parallel experimental sets with samples stored at 4 and 22 °C, respectively. Whole blood CL was estimated at 2, 6, 24, 48, 72, 96 and 120 h after collection. The study demonstrated that temperature and duration of sample storage are factors that determine the quality of CL measurements of whole blood in horses. The study concluded that samples should be stored at 4 °C and the assay should be performed as early as possible. It was also shown that the viability period of horse blood for CL assays is relatively long. Material stored at room temperature for 24 h and even up to 48 h at 4 °C did not show any significant decrease in spontaneous or stimulated chemiluminescence. PMID:22730351

Krumrych, Wies?aw; Skórzewski, Rados?aw; Malinowski, Edward

2013-01-01

191

Chemiluminescence Arising from Simultaneous Transitions in Pairs of Singlet Oxygen Molecules.  

National Technical Information Service (NTIS)

A complete spectroscopic comparison is made of the numerous chemiluminescence bands observed in the 20C aqueous solution reaction of hydrogen peroxide with hypochlorite ion, with gaseous molecular oxygen absorption bands at high pressure. Both single mole...

A. U. Khan M. Kasha

1969-01-01

192

Chemiluminescence and bioluminescence microbe detection  

NASA Technical Reports Server (NTRS)

Automated biosensors for online use with NASA Water Monitoring System employs bioluminescence and chemiluminescence techniques to rapidly measure microbe contamination of water samples. System eliminates standard laboratory procedures requiring time duration of 24 hours or longer.

Taylor, R. E.; Chappelle, E.; Picciolo, G. L.; Jeffers, E. L.; Thomas, R. R.

1978-01-01

193

CHEMILUMINESCENCE MEASUREMENT OF REACTIVITY WEIGHTED ETHYLENE-EQUIVALENT HYDROCARBONS  

EPA Science Inventory

A reactive hydrocarbon analyzer (RHA), based on the chemiluminescent reaction of hydrocarbons with oxygen atoms, is used to provide a rapid indication of reactivity weighted hydrocarbon mass in automobile exhaust. Samples are reported by their ethylene-equivalent concentration--t...

194

Chemiluminescent Spectra of YbF and YbCl.  

National Technical Information Service (NTIS)

The chemiluminescent spectra of YbF and YbCl are obtained from the reactions of ytterbium with fluorine and ytterbium with chlorine dioxide under beam-gas conditions and in a high-pressure (3 Torr) system. Much more extensive band systems are observed tha...

H. U. Lee R. N. Zare

1977-01-01

195

Chemiluminescence detection of five-membered heteroaromatic compounds using electrogenerated tris(2,2'-bipyridine)-ruthenium(III).  

PubMed

Heteroaromatic compounds, such as 2,5-dimethylthiophene, 2,5-dimethylpyrrole, and 2,5-dimethylfuran, were found to act as reducing agents for the tris(2,2'-bipyridine)ruthenium(III), Ru(bpy)3(3+), chemiluminescent reaction. In order to characterize the chemiluminescent reaction of Ru(bpy)3(3+) with heteroaromatic compounds, we have investigated various mono-, di-, and tri-heteroaromatic compounds. The pi-electron density and stability of aromatic rings influence the chemiluminescent efficiency of the reaction. Above all, 2,5-dimethylthiophene produced strong chemiluminescence under acidic conditions. In addition, we confirmed that the rate of the chemiluminescent reaction of Ru(bpy)3(3+) with 2,5-dimethylthiophene is very fast. PMID:17420543

Kodamatani, Hitoshi; Komatsu, Yu; Yamazaki, Shigeo; Saito, Keiitsu

2007-04-01

196

Chemiluminescence of ischaemic and reperfused intestine in vivo.  

PubMed Central

Low level chemiluminescence of exposed rat intestine was measured during occlusive ischaemia and reperfusion. Spontaneous emission of in vivo rat intestine (10 +/- 1 cps/cm2) decreased almost to zero in animals subjected to ischaemia and when the period of ischaemia lasted only two minutes, chemiluminescence increased beyond control levels (39%, three minutes after reperfusion) at intestine deligation. This overshoot did not occur when rats were pretreated with allopurinol (40 + 100 mg/kg bw). The ratio of xanthine dehydrogenase to xanthine oxidase activities was 3.46 in preischaemic intestine samples. The same ratio was changed to 0.35 in samples subjected to two minutes of ischaemia. As chemiluminescence appears to reflect the steady state level of singlet oxygen, which in turn derives from the steady state level of peroxy radicals, these results agree with the view that oxygen radicals derived from the xanthine oxidase reaction are involved in the cellular damage produced after ischaemia and reoxygenation in the intestine.

Roldan, E J; Pinus, C R; Turrens, J F; Boveris, A

1989-01-01

197

Determination Co 2+ in vitamin B 12 based on enhancement of 2-(4-substituted-phenyl)-4,5-di(2-furyl) imidazole and H 2O 2 chemiluminescence reaction  

NASA Astrophysics Data System (ADS)

In this paper, three kinds of imidazole derivatives, 2-(4-methylphenyl)-4,5-di(2-furyl) imidazole (MDFI), 2-(4-nitrophenyl)-4,5-di(2-furyl) imidazole (NDFI), and 2-(4-tert-butylphenyl)-4,5-di(2-furyl) imidazole (t-BDFI) were synthesized. In an alkaline medium, the chemiluminescence (CL) reaction of imidazole derivatives with H 2O 2 has been investigated. It was also found that MDFI/H 2O 2 and t-BDFI/H 2O 2 systems gave strong CL. When Co 2+ was added into the two CL systems, the CL intensity was remarkably enhanced. In the optimum conditions, the CL intensity is linearly related to the logarithm of concentration of Co 2+. The linear ranges are 5 × 10 -9-2.5 × 10 -7 mol/L for MDFI/H 2O 2 system and 5 × 10 -9-2.5 × 10 -7 mol/L for t-BDFI/H 2O 2 system, and the corresponding detection limits are 1.2 × 10 -9 mol/L and 1.1 × 10 -9 mol/L, respectively. The method was applied to the determination of Co 2+ in vitamin B 12 injection. Furthermore, the CL mechanism was also discussed.

Han, Lu; Zhang, Yumin; Kang, Jing; Tang, Jieli; Zhang, Yihua

2011-11-01

198

40 CFR 1065.270 - Chemiluminescent detector.  

Code of Federal Regulations, 2010 CFR

...ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR POLLUTION CONTROLS ENGINE-TESTING PROCEDURES Measurement Instruments Nox and N2o Measurements § 1065.270 Chemiluminescent detector. (a) Application. You may use a chemiluminescent detector...

2010-07-01

199

Stimulus specific effect of ibuprofen on chemiluminescence of sheep neutrophils  

SciTech Connect

The authors have shown that pretreatment with ibuprofen inhibits free radical release from complement stimulated neutrophils. To further examine the effect of ibuprofen on neutrophil free radical release, they stimulated neutrophils with the synthetic peptide, FMLP, phorbol myristate acetate (PMA), or zymosan-activated plasma (ZAP). Pure (>95%), viable (>95%) sheep neutrophils (2 x 10/sup 6/) were placed in HEPES buffer, luminol, drug or vehicle and stimulated in the luminometer with one of the stimuli. The chemiluminescence (CL) response was recorded and the drug treated samples were compared to vehicle treated controls. Ibuprofen had a dose dependent effect on CL in ZAP stimulated neutrophils. At the highest dose (10/sup -2/M) these cells produced only 37 +/- 7% of the CL response observed in the control cells. In contrast, at the same dose, ibuprofen did not significantly attenuate CL seen in FMLP stimulated cells, with these cells producing 79 +/- 7% of the control cells; nor did ibuprofen effect PMA stimulated CL, as these cells produced a CL response that was 85 +/- 8% of the control cells. Ibuprofen appears to have a stimulus specific effect on free radical release in activated neutrophils. It is also apparent that ibuprofen inhibits complement stimulated free radical release by some mechanism independent of its cyclooxygenase inhibitory effect.

Tahamont, M.V.; Margiotta, M.; Gee, M.H.

1986-03-05

200

Infrared Chemiluminescence from the Reaction of Hydrogen Atoms with SCl2, S2Cl2, SOCl2, SO2Cl2, and OCl2  

Microsoft Academic Search

The hydrogen chloride infrared emission from the reactions of H atoms with OCl2, SCl2, S2Cl2, SOCl2, and SO2Cl2 has been studied under a variety of conditions. A limited number of experiments with the deuterium atom reactions also are reported. The emission from the highest vibrational level gave an estimate of the first sulfur–chlorine bond dissociation energy for these compounds. The

M. J. Perona; D. W. Setser; R. J. JOHNSONt

1970-01-01

201

Simultaneous speciation of iron(II) and iron(III) by ion chromatography with chemiluminescence detection.  

PubMed

This study reports on a method for the speciation of iron in aqueous samples by the simultaneous analysis of divalent and trivalent iron ions with ion chromatography equipped with chemiluminescence detection (IC-CLD). Ferrous and ferric ions are first chelated by pyridine-2,6-dicarboxylic acid (PDCA) to form complexed anions, and separated by a mixed-bed ion-exchange column. The separated complexed ions are then detected with a CLD system containing luminol and hydrogen peroxide in a basic solution. This luminescence system has a linear dynamic range of ca. 3 orders of magnitude, with method detection limits as low as 7 µg L(-1) for Fe(II) and 3 µg L(-1) for Fe(III), measured in the simultaneous detection mode. This system resists interferences from common cations such as Cd, Ca, Cr, Cu, Mg, Ni, Pb, and Zn. Evaluation by analyzing real samples shows that this method is rapid, accurate, sensitive, and selective. PMID:22878635

Chen, Yun-Chieh; Jian, Yu-Ling; Chiu, Kong-Hwa; Yak, Hwa-Kwang

2012-01-01

202

A highly sensitive chemiluminescence assay for superoxide detection and chronic granulomatous disease diagnosis.  

PubMed

Reactive oxygen species (ROS) produced by neutrophils are crucial for defense against infectious diseases, and the adequate measurement of ROS levels is an important way to evaluate the possibility of infections. The fluorescent probe dihydrorhodamine 123 has been applied exclusively to the measurement of ROS thus far. We developed a novel method for detecting ROS, which utilizes the chemiluminescent probes Luminol and Diogenes. The new method quantitatively detects ROS produced by as few as 10 to 10(4) neutrophils. Furthermore, this method can detect ROS levels in one microliter of whole blood or ROS produced by Epstein-Barr immortalized B lymphocytes. This method will be valuable for prompt diagnosis of neonatal chronic granulomatous diseases in which neutrophils aberrantly produce superoxide. PMID:22028609

Yamazaki, Tsuyoshi; Kawai, Chikage; Yamauchi, Akira; Kuribayashi, Futoshi

2011-06-01

203

Comparison of chemiluminescence methods for analysis of hydrogen peroxide and hydroxyl radicals  

NASA Astrophysics Data System (ADS)

Assessment of alpha radiolysis influence on the chemistry of geologically disposed spent fuel demands analytical methods for radiolytic product determination at trace levels. Several chemiluminescence methods for the detection of radiolytic oxidants hydrogen peroxide and hydroxyl radicals are tested. Two of hydrogen peroxide methods use luminol, catalyzed by either ?-peroxidase or hemin, one uses 10-methyl-9-(p-formylphenyl)-acridinium carboxylate trifluoromethanesulfonate and one potassium periodate. All recipes are tested as batch systems in basic conditions. For hydroxyl radical detection luminophores selected are 3-hydroxyphthalic hydrazide and rutin. Both methods are tested as batch systems. The results are compared and the applicability of the methods for near-field dissolution studies is discussed.

Pehrman, R.; Amme, M.; Cachoir, C.

2006-01-01

204

3D-printed and CNC milled flow-cells for chemiluminescence detection.  

PubMed

Herein we explore modern fabrication techniques for the development of chemiluminescence detection flow-cells with features not attainable using the traditional coiled tubing approach. This includes the first 3D-printed chemiluminescence flow-cells, and a milled flow-cell designed to split the analyte stream into two separate detection zones within the same polymer chip. The flow-cells are compared to conventional detection systems using flow injection analysis (FIA) and high performance liquid chromatography (HPLC), with the fast chemiluminescence reactions of an acidic potassium permanganate reagent with morphine and a series of adrenergic phenolic amines. PMID:24881540

Spilstead, Kara B; Learey, Jessica J; Doeven, Egan H; Barbante, Gregory J; Mohr, Stephan; Barnett, Neil W; Terry, Jessica M; Hall, Robynne M; Francis, Paul S

2014-08-01

205

Flow-injection chemiluminescence determination of gentamicin: optimization by central composite design.  

PubMed

A simple and sensitive flow-injection chemiluminescence (CL) method has been developed for the determination of gentamicin sulfate. The method is based on the inhibitory effect of gentamicin on the CL emission accompanying oxidation of luminol by H2 O2 in an alkaline medium in the presence of Cu(II) as a catalyst. Inhibition was caused by the formation of a strong complex between analyte and the catalyst. Experimental variables, including the concentrations of luminol (µmol/L), H2 O2 (mol/L), Cu(II) (mol/L) and NaOH (mol/L), were optimized using a central composite design. Under optimum conditions, the plot of CL intensity versus gentamicin concentration was found to have two linear ranges. One range was at low concentrations from 1.0 to 10.0?mg/L and the other was from 10.0 to 30.0?mg/L. Precision was calculated by analyzing samples containing 5.0?mg/L gentamicin (n?=?11) and the relative standard deviation (RSD) was 1.7%. Also, a high injection throughput of 120 samples/h was achieved. This method was successfully applied to the determination of gentamicin sulfate in pharmaceutical formulations and water samples. PMID:23744581

Iranifam, M; Hasanzadeh, A; Fathinia, M; Khataee, A R; Mousavi, S A

2014-05-01

206

Highly sensitive luminol electrochemiluminescence immunosensor based on ZnO nanoparticles and glucose oxidase decorated graphene for cancer biomarker detection.  

PubMed

In this work, we reported a sandwiched luminol electrochemiluminescence (ECL) immunosensor using ZnO nanoparticles (ZnONPs) and glucose oxidase (GOD) decorated graphene as labels and in situ generated hydrogen peroxide as coreactant. In order to construct the base of the immunosensor, a hybrid architecture of Au nanoparticles and graphene by reduction of HAuCl(4) and graphene oxide (GO) with ascorbic acid was prepared. The resulted hybrid architecture modified electrode provided an excellent platform for immobilization of antibody with good bioactivity and stability. Then, ZnONPs and GOD functionalized graphene labeled secondary antibody was designed for fabricating a novel sandwiched ECL immunosensor. Enhanced sensitivity was obtained by in situ generating hydrogen peroxide with glucose oxidase and the catalysis of ZnONPs to the ECL reaction of luminol-H(2)O(2) system. The as-prepared ECL immunosensor exhibited excellent analytical property for the detection of carcinoembryonic antigen (CEA) in the range from 10 pg mL(-1) to 80 ng mL(-1) and with a detection limit of 3.3 pg mL(-1) (SN(-1)=3). The amplification strategy performed good promise for clinical application of screening of cancer biomarkers. PMID:22938618

Cheng, Yinfeng; Yuan, Ruo; Chai, Yaqin; Niu, Huan; Cao, Yaling; Liu, Huijing; Bai, Lijuan; Yuan, Yali

2012-10-01

207

Supramolecular assembly and nanostructures of a series of luminol derivatives with aromatic/alkyl substituted groups in Langmuir-Blodgett films.  

PubMed

A series of functional luminol derivatives with aromatic and alkyl substituted groups has been designed and synthesized from the reaction of the corresponding chloride precursors with luminol. These compounds can be spread on water surface to form stable Langmuir films at the air-water interface. It has been found that UV and IR spectra confirmed the characteristic aromatic segment, imide group, and aromatic/alkyl substituted groups. In addition, for the interfacial assembly process of compounds with alkyl substituted groups, there are obvious spectral changes for the alkyl chains. AFM results indicated that various different aggregated domains may be fabricated in the transferred LB films. For all cases, the substituted groups in molecular structures have an important effect in regulating the aggregation mode and spectral changes in organized molecular films. The present results showed that the modified luminol derivatives may have potential application in functional material fields such as ECL sensor, which may give some insight to study the relationship between the molecular structures and supramolecular aggregation of amphiphiles in organized molecular films. PMID:24738403

Jiao, Tifeng; Xing, Yuanyuan; Zhang, Qingrui; Zhang, Li; Liu, Minghua; Zhou, Jingxin; Gao, Faming

2014-06-01

208

Chemiluminescent flow-through sensor for 1,10-phenanthroline based on the combination of molecular imprinting and chemiluminescence.  

PubMed

A functional polymer for the catalysis of the chemiluminescent reaction and molecular recognition ability of 1,10-phenanthroline was prepared based on the molecular imprinting method. The structural and catalytic roles of transition metal ion interactions were applied in the material design. A ternary complex, 4-vinylpyridine-Cu(II)-1,10-phenanthroline (2:1:1), was synthesized and used as a functional monomer. The ligand 1,10-phenanthroline in the ternary complex was the template used to form the molecularly imprinted polymer. Another monomer, styrene, and the cross-linking reagent divinylbenzene were copolymerized with the ternary complex. The polymer containing the ternary complex is an efficient catalyst for the decomposition of hydrogen peroxide. During the hydrogen peroxide decomposition, superoxide radical ion is formed, which reacts with 1,10-phenanthroline and gives a chemiluminescent emission. The 1,10-phenanthroline was destroyed during the chemiluminescent reaction, leaving a cavity and copper binding sites for another 1,10-phenanthroline molecule. The prepared polymer particles were packed into a glass tube and developed as a molecular recognition chemiluminescent flow-through sensor for 1,10-phenanthroline. The sensitivity and selectivity of the sensor were tested. PMID:11445942

Lin, J M; Yamada, M

2001-06-01

209

The Kinetics and Dynamics of AN Atmospheric Reaction System Using Chemiluminescence, Laser-Induced Fluorescence and Stimulated Raman Excitation: Hydrogen Sulfide + Nitrous Oxide  

Microsoft Academic Search

Acid rain and the greenhouse effect are two of the most important environmental issues of this century. Oxidation of reduced sulfur compounds is responsible for acid rain, and the increase in concentration and subsequent reactivity of vibrationally excited greenhouse gases are important to understand global warming. The following reaction system addresses these two issues and forms the basis for the

K. Ravichandran

1995-01-01

210

Chemiluminescence detection coupled to liquid chromatography for the determination of penicillamine in human urine  

Microsoft Academic Search

An on-line liquid chromatography (LC) chemiluminescence detection system is proposed for the determination of penicillamine in human urine. Ion-pair reversed-phase LC using octane sulphonic acid as ion-pairing reagent allows the separation of penicillamine from the urine matrix. The Chemiluminescence reaction of penicillamine with Ce(IV) in sulphuric acid sensitized by quinine is suggested for sensitive penicillamine detection in a post-column mixing

Z. Zhang; W. R. G. Baeyens; X. Zhang; Y. Zhao; G. Van Der Weken

1997-01-01

211

Comparative application of sulfur chemiluminescence detection in gas and supercritical fluid chromatography  

Microsoft Academic Search

Sulfur chemiluminescence detection (SCD) for the determination of sulfur-containing compounds in gas chromatography (GC) and supercritical fluid chromatography (SFC) is described. The detector is based on the chemiluminescence reaction of ozone with sulfur monoxide which is produced in an H2\\/O2 reducing flame from sulfur-containing analytes. High selectivity over nonsulfur-containing species has been achieved for a number of mercaptans and sulfur-containing

W. F. Sye; Z. X. Zhao; M. L. Lee

1992-01-01

212

Modeling and analysis of chemiluminescence sensing for syngas, methane and Jet-A combustion  

NASA Astrophysics Data System (ADS)

Flame chemiluminescence has received increasing attention for its potential sensor and diagnostic applications in combustors. A number of studies have used flame chemiluminescence to monitor flame status, and combustor performance. While most of these studies have been empirical in nature, chemiluminescence modeling has the potential to provide a better understanding of the chemiluminescence processes and their dependence on various combustion operating conditions. The primary objective of this research was to identify and validate the important chemiluminescence reaction mechanisms for OH*, CH* and CO2 *. To this end, measurements were performed at various operating conditions, primarily in laminar, premixed flames, fueled with methane, syngas (H 2/CO) and Jet-A. The results are compared to 1-d laminar flame simulations employing the chemiluminescence mechanisms. The secondary objective was to use the experiments and validated chemiluminescence reaction mechanisms to evaluate the usefulness of flame chemiluminescence as a combustion diagnostic, particularly for heat release rate and equivalence ratio. The validation studies were able to identify specific mechanisms for OH*, CH* and CO2* that produced excellent agreement with the experimental data in most cases. The mechanisms were able to predict the variation of the chemiluminescence signals with equivalence ratio but not with pressure and reactant preheat. The reasons causing this disagreement could be due to the inaccuracies in the basic chemical mechanism used in the simulations, lack of accurate quenching data (for CH*), thermal excitation (for OH*) and radiative trapping (for OH* and CO2*) and interference from the emissions of other species (such as HCO and H2O), for CO2*. Regarding the utility of chemiluminescence for sensing, a number of observations can be made. In syngas-air flames, CO2* is a reasonable heat release rate marker, at least for very lean conditions. OH* shows some advantage in atmospheric-pressure methane and Jet-A flames in general, while CH* is advantageous at high pressure and very lean conditions at atmospheric pressure. The CO 2*/OH* intensity ratio is not useful for sensing equivalence ratio in syngas flames, except maybe at very lean conditions. However, the CH*/OH* signal ratio is a promising approach for sensing equivalence ratio at low or very high pressure conditions in hydrocarbon flames. Thermal excitation and self-absorption processes for OH* chemiluminescence can become important for combustors operating at high pressure, high preheat and near stoichiometric conditions. Background subtracted chemiluminescence signals are recommended for sensing purposes.

Nori, Venkata Narasimham

213

Chemiluminescence of Organic Peroxide Decomposition  

Microsoft Academic Search

The thermal decomposition of organic hydroperoxides in solution gives rise to weak chemiluminescence effects. One emission, which has a maximum at a wavelength longer than 5500 Å, seems chiefly, if not entirely, a surface effect. With hydroperoxides having aromatic nuclei a blue emission from the liquid volume is observed. The nature of the light-emitting processes is discussed.

E. J. Bowen; R. A. Lloyd

1963-01-01

214

Microwave trigger metal-enhanced chemiluminescence (MT MEC) and spatial and temporal control of same  

US Patent & Trademark Office Database

The present invention relates to a method of imaging structures and features using plasmonic emissions from metallic surfaces caused by chemiluminescence based chemical and biological reactions wherein imaging of the reactions is enhanced by the use of microwave energy and further enhanced by using metallic geometric structures for spatially and temporally controlling the biological and chemical reactions.

2011-08-30

215

Kinetics and selectivity of permanganate chemiluminescence: a study of hydroxyl and amino disubstituted benzene positional isomers.  

PubMed

Examination of the chemiluminescence reactions of dihydroxybenzenes, aminophenols and phenylenediamines with acidic potassium permanganate has provided a new understanding of the relationships between analyte structure, reaction conditions, kinetics of the light-producing pathway and emission intensity, with broad implications for this widely utilised chemiluminescence detection system. Using a permanganate reagent prepared in a polyphosphate solution and adjusted to pH 2.5, large differences in the rate of reaction with different positional isomers were observed, with the meta-substituted forms reacting far slower and therefore exhibiting much lower chemiluminescence intensities in flow analysis systems. The preliminary partial reduction of permanganate to form significant concentrations of Mn(III) increased the rate of reaction with all analytes tested, resulting in comparable or (in the case of aminophenol and phenylenediamine) even greater emission intensities for the meta-isomers, demonstrating the opportunity to tune the selectivity of the reagent towards certain classes of compound or even specific positional isomers of the same compound. Using more acidic permanganate reagents, in which polyphosphates are not required, the discrepancy between the chemiluminescence intensities was still observed, but was less prominent due to the generally faster rates of reaction. The enhancement of these chemiluminescence reactions by on-line addition of formic acid or formaldehyde can in part also be attributed to the generation of significant pools of the key Mn(III) precursor to the emitting species. PMID:22027128

Slezak, Teo; Smith, Zoe M; Adcock, Jacqui L; Hindson, Christopher M; Barnett, Neil W; Nesterenko, Pavel N; Francis, Paul S

2011-11-30

216

Stimulation of chemiluminescence and resistance against aerogenic influenza virus infection by synthetic muramyl dipeptide combined with trehalose dimycolate.  

PubMed Central

The effect on respiratory burst of splenic cells from mice pretreated with oil-in-water emulsions of muramyl dipeptide (MDP), trehalose dimycolate (TDM), or the combination of MDP with TDM was studied by luminol-dependent chemiluminescence in response to stimulation by zymosan. Spleen cells from mice pretreated with TDM, but not those of mice treated with MDP, generated increased chemiluminescence. Spleen cells from animals pretreated with the combination of MDP and TDM exhibited markedly enhanced chemiluminescence activity. The effect of enhanced activity of preparations containing MDP combined with TDM was further examined in vivo by an aerosol infection of pretreated mice with a mouse-pathogenic influenza virus. Pretreatment with 6-O-acyl analogs and one ubiquinone derivative of MDP alone did not induce any resistance against influenza virus. Significant protection was conferred only when MDP and certain analogs were combined with TDM. The enhancement of nonspecific resistance to influenza virus infection was related to the chemical structure of the synthetic immunostimulant. A greater degree of protection was induced by the combination of TDM with the lipophilic derivatives like B 30-MDP and L-18 MDP.

Masihi, K N; Brehmer, W; Azuma, I; Lange, W; Muller, S

1984-01-01

217

A novel high selectivity chemiluminescence sensor for fenvalerate based on double-sided hollow molecularly imprinted materials.  

PubMed

Novel fenvalerate double-sided hollow molecularly imprinted microspheres (fenvalerate-DHMIMs) were fabricated by in situ polymerization with the help of mesoporous silica microspheres (MSMs) in this paper for the very first time. Scanning electron microscope was employed to characterize the surface morphology of the fenvalerate-DHMIMs. Taking advantage of the quenching effect of fenvalerate on the luminol-H(2)O(2)-NaOH chemiluminescence system, a new model was established to determine fenvalerate by a highly selective flow injection chemiluminescence method. The traditional flow-through cell was replaced by a novel Y-shaped column. The chemiluminescence intensity was linear with fenvalerate concentration over the range of 5.0 × 10(-8) to 2.0 × 10(-5) g mL(-1) and the detection limit was 2.2 × 10(-8) g mL(-1). The relative standard deviation (RSD) for the determination of 2.0 × 10(-6) g mL(-1) fenvalerate was 1.4% (n = 11). The proposed method was applied to the determination of fenvalerate in real samples with satisfactory results. PMID:22852133

Zang, Dejin; Yan, Mei; Zhao, Peini; Ge, Lei; Liu, Shiquan; Yu, Jinghua

2012-09-21

218

Using a large area CMOS APS for direct chemiluminescence detection in Western blotting electrophoresis  

NASA Astrophysics Data System (ADS)

Western blotting electrophoretic sequencing is an analytical technique widely used in Functional Proteomics to detect, recognize and quantify specific labelled proteins in biological samples. A commonly used label for western blotting is Enhanced ChemiLuminescence (ECL) reagents based on fluorescent light emission of Luminol at 425nm. Film emulsion is the conventional detection medium, but is characterized by non-linear response and limited dynamic range. Several western blotting digital imaging systems have being developed, mainly based on the use of cooled Charge Coupled Devices (CCDs) and single avalanche diodes that address these issues. Even so these systems present key drawbacks, such as a low frame rate and require operation at low temperature. Direct optical detection using Complementary Metal Oxide Semiconductor (CMOS) Active Pixel Sensors (APS)could represent a suitable digital alternative for this application. In this paper the authors demonstrate the viability of direct chemiluminescent light detection in western blotting electrophoresis using a CMOS APS at room temperature. Furthermore, in recent years, improvements in fabrication techniques have made available reliable processes for very large imagers, which can be now scaled up to wafer size, allowing direct contact imaging of full size western blotting samples. We propose using a novel wafer scale APS (12.8 cm×13.2 cm), with an array architecture using two different pixel geometries that can deliver an inherently low noise and high dynamic range image at the same time representing a dramatic improvement with respect to the current western blotting imaging systems.

Esposito, Michela; Newcombe, Jane; Anaxagoras, Thalis; Allinson, Nigel M.; Wells, Kevin

2012-02-01

219

A magnetic particles-based chemiluminescence enzyme immunoassay for rapid detection of ovalbumin.  

PubMed

Egg allergy is an important public health and safety concern, so quantification and administration of food or vaccines containing ovalbumin (OVA) are urgently needed. This study aimed to establish a rapid and sensitive magnetic particles-chemiluminescence enzyme immunoassay (MPs-CLEIA) for the determination of OVA. The proposed method was developed on the basis of a double antibodies sandwich immunoreaction and luminol-H2O2 chemiluminescence system. The MPs served as both the solid phase and separator, the anti-OVA MPs-coated polyclonal antibodies (pAbs) were used as capturing antibody, and the horseradish peroxidase (HRP)-labeled monoclonal antibody (mAb) was taken as detecting antibody. The parameters of the method were evaluated and optimized. The established MPs-CLEIA method had a linear range from 0.31 to 100ng/ml with a detection limit of 0.24ng/ml. The assays showed low reactivities and less than 5% of intraassay and interassay coefficients of variation (CVs), and the average recoveries were between 92 and 97%. Furthermore, the developed method was applied in real samples analysis successfully, and the correlation coefficient with the commercially available OVA kit was 0.9976. Moreover, it was more rapid and sensitive compared with the other methods for testing OVA. PMID:24769049

Feng, Xiao-Li; Ren, Hong-Lin; Li, Yan-Song; Hu, Pan; Zhou, Yu; Liu, Zeng-Shan; Yan, Dong-Ming; Hui, Qi; Liu, Dong; Lin, Chao; Liu, Nan-Nan; Liu, Yan-Yan; Lu, Shi-Ying

2014-08-15

220

The mechanism of specific inhibition of stimulated chemiluminescence of polymorphonuclear blood leukocytes in allergic processes.  

PubMed

In the first part of our work (1) it was found that the cultivation of blood of sensitized people and animals with specific allergen (AI) caused the phenomenon of specific inhibition stimulated luminol-dependent chemiluminescence of leukocytes (PhSISCL). In the current study the mechanism of this inhibition was investigated. It was revealed that PhSISCL resulted from the direct influence of AI on polymorphonuclear leukocytes (PMNL). The activity of NADPH-dependent oxidase was established by the Wymann M. et al. method (2). It was determined that the values of chemiluminescence (CL) for the cultivation of sensitized rabbit blood with specific AI did not differ from the control. This demonstrated that the activity of NADPH-oxidase was not inhibited. On these grounds it is possible to assume that PhSISCL is connected to either the inhibition of myeloperoxidase (MPO) activity or to a release of this ferment from PMNL during cultivation. The latter was specifically investigated and was not confirmed. PMID:8959544

Pytsky, V I; Filatov, O Y

1996-01-01

221

Quantification of 2,4-dichlorophenoxyacetic acid in oranges and mandarins by chemiluminescent ELISA.  

PubMed

Direct competitive enzyme-linked immunosorbent assay (ELISA) for 2,4-dichlorophenoxyacetic acid (2,4-D) was developed. Varying the concentrations of monoclonal anti-2,4-D-antibody and the conjugate of soybean peroxidase and 2,4-D the conditions of ELISA performance were optimised. The chemiluminescent method based on peroxidase-catalysed oxidation of luminol was applied to measure the enzyme activity of the conjugate. A mixture of 3-(10'-phenothiazinyl)propane-1-sulfonate and 4-morpholinopyridine was used as potent enhancer of chemiluminescence signal. It was shown that the values of the lower detection limit, IC50 and the working range were 1.5, 64.0, and 6.5-545ng/mL, respectively. The recovery values of CL-ELISA from 10 spiked samples of oranges (n=5) and mandarins (n=5) cultivated in green house without use of 2,4-D and containing different 2,4-D concentrations (10-300ng/mL) were ranged from 92% to 104% that indicated on the absence of matrix effect for the fruit extracts of interest. Determination of 2,4-D in peel of five oranges and five mandarins purchased from stores in Vietnam showed that 2,4-D content in oranges fruits (79-104?g/kg) was significantly higher than that in mandarins (1.66-2.82?g/kg). PMID:23790860

Vdovenko, Marina M; Stepanova, Alexandra S; Eremin, Sergei A; Van Cuong, Nguyen; Uskova, Natalia A; Yu Sakharov, Ivan

2013-11-15

222

Isoprene measurement by ozone-induced chemiluminescence  

SciTech Connect

An instrument has been constructed that monitors gaseous isoprene continuously. The basis for detection is chemiluminescence with ozone. The isoprene/ozone reaction produces electronically excited formaldehyde whose subsequent emission to the ground state is viewed with a blue-sensitive photomultiplier tube. The instrument has a response time of 0.1 s, is linear over 3 orders of magnitude, and has a detection limit for isoprene of 400 pptv (at S/N = 2 and 5-s electronic time constant). Selectivities over various alkenes and other compounds are presented. The first real-time isoprene fluxes from oak leaves, using a single living leaf, are measured as a function of light modulation.

Hills, A.J.; Zimmerman, P.R. (National Center for Atmospheric Research, Boulder, CO (USA))

1990-05-15

223

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors  

PubMed Central

Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) involves the generation of species at electrode surfaces that then undergo electron-transfer reactions to form excited states that emit light. ECL biosensor, combining advantages offered by the selectivity of the biological recognition elements and the sensitivity of ECL technique, is a powerful device for ultrasensitive biomolecule detection and quantification. Nanomaterials are of considerable interest in the biosensor field owing to their unique physical and chemical properties, which have led to novel biosensors that have exhibited high sensitivity and stability. Nanomaterials including nanoparticles and nanotubes, prepared from metals, semiconductor, carbon or polymeric species, have been widely investigated for their ability to enhance the efficiencies of ECL biosensors, such as taking as modification electrode materials, or as carrier of ECL labels and ECL-emitting species. Particularly useful application of nanomaterials in ECL biosensors with emphasis on the years 2004-2008 is reviewed. Remarks on application of nanomaterials in ECL biosensors are also surveyed.

Qi, Honglan; Peng, Yage; Gao, Qiang; Zhang, Chengxiao

2009-01-01

224

Ozone chemiluminescent detection of olefins: Potential applications for real-time measurements of natural hydrocarbon emissions  

SciTech Connect

A chemiluminescence analyzer has been constructed that takes advantage of the temperature dependence of the ozone-hydrocarbon reaction. When operated at a temperature of 170 C, the analyzer functions as a total nonmethane hydrocarbon analyzer with sensitivities 10--1,000 times better than a conventional FID. However, with operation at varying temperatures, the chemiluminescent signal reflects the differences in rates of reaction of the hydrocarbons with ozone. Preliminary studies at room temperature indicated that the relative rates of reaction of isoprene, {alpha}-pinene, {beta}-pinene, and limonene with ozone correlated with the observed chemiluminescence signal. When hydrocarbons are grouped in classes of similar structure, their rates of reaction with electrophilic atmospheric oxidants (e.g., OH, O{sub 3}, NO{sub 3}) can be correlated with each other. By varying the temperature of the reaction chamber, the chemiluminescence analyzer can be tuned to more reactive classes of hydrocarbons. Therefore, the chemiluminescence analyzer has the ability to determine atmospheric hydrocarbon concentrations as a function of class and will also provide a measure of the atmospheric reactivity of the hydrocarbons.

Marley, N.A.; Gaffney, J.S.; Cunningham, M.M.

1997-10-01

225

Rapid imaging of oxygen free radicals produced in PDT using FCLA chemiluminescence probe  

NASA Astrophysics Data System (ADS)

In this paper, we report a rapid imaging method for oxygen free radicals produced in photodynamic therapy (PDT) in vivo using chemiluminescence probe. The mechanism for PDT involves singlet oxygen (1O2) generated by energy transfer from photosensitizers. 1O2 can react with FCLA, which is a specific chemiluminescence probe for detecting 1O2 and superoxide (O2-). The reaction of FCLA (Fluoresceinyl Cypridina Luminescent Analog) and 1O2 can give emission with peak wavelength at about 532 nm. In the present study, FCLA was chosen as an optical reportere of 1O2 produced from photosensitization reaction of Hematoporphyrin Derivative (HpD) in model solution and in nude mice with transplanted mammary cancer. Photosensitized chemiluminescence from the reaction of FCLA with 1O2 was detected by a highly sensitive Intensified Charge-Coupled Device (ICCD) detector. The chemiluminescence was markedly inhibited by the addition of 10mmol/L sodium azide (NaN3) to the model solution and minor effects were observed at the addition of 10 micrometers ol/L superoxide dismutase (SOD), 20 mmol/L mannitol and 100 (mu) g/mL catalase, respectively, thus indicating that 1O2 generation from photosensitization reaction mainly results in the light emission. Experiments in vivo with tumor-bearing mouse showed a clear chemiluminescence image of tumor.

Xing, Da; Wang, Juan; He, Yonghong

2002-09-01

226

A rapid chemiluminescent method for quantitation of human DNA.  

PubMed Central

A sensitive and simple method for the quantitation of human DNA is described. This method is based on probe hybridization to a human alpha satellite locus, D17Z1. The biotinylated probe is hybridized to sample DNA immobilized on nylon membrane. The subsequent binding of streptavidin-horseradish peroxidase to the bound probe allows for chemiluminescent detection using a luminol-based reagent and X-ray film. Less than 150 pg of human DNA can easily be detected with a 15 minute exposure. The entire procedure can be performed in 1.5 hours. Microgram quantities of nonhuman DNA have been tested and the results indicate very high specificity for human DNA. The data on film can be scanned into a computer and a commercially available program can be used to create a standard curve where DNA quantity is plotted against the mean density of each slot blot signal. The methods described can also be applied to the very sensitive determination of quantity and quality (size) of DNA on Southern blots. The high sensitivity of this quantitation method requires the consumption of only a fraction of sample for analysis. Determination of DNA quantity is necessary for RFLP and many PCR-based tests where optimal results are obtained only with a relatively narrow range of DNA quantities. The specificity of this quantitation method for human DNA will be useful for the analysis of samples that may also contain bacterial or other non-human DNA, for example forensic evidence samples, ancient DNA samples, or clinical samples. Images

Walsh, P S; Varlaro, J; Reynolds, R

1992-01-01

227

Effects of 4-hydroxynonenal on isolated hepatocytes. Studies on chemiluminescence response, alkane production and glutathione status.  

PubMed Central

The effect of 4-hydroxy-2,3-trans-nonenal, a diffusible product of lipid peroxidation, on isolated hepatocytes was evaluated with two non-invasive techniques measuring low-level chemiluminescence and alkane evolution. Oxygen-induced low-level chemiluminescence and ethane and n-pentane formation by hepatocytes is enhanced over 7-fold in the presence of 4-hydroxynonenal (2 mM). Glutathione-depleted hepatocytes show a higher increase than controls in both low-level chemiluminescence and alkane formation upon supplementation with 4-hydroxynonenal. The effects on both parameters are diminished by vitamin E pretreatment of rats and are absent under anaerobiosis. At variance with chemiluminescence and alkane formation, 4-hydroxynonenal does not elicit a concomitant increase in malonaldehyde or diene-conjugate formation. Addition of 4-hydroxynonenal to a suspension of hepatocytes causes a rapid loss of cellular glutathione in the form of a glutathione conjugate with the alkenal as observed with high-pressure liquid-chromatographic analysis. The reaction between glutathione and 4-hydroxynonenal proceeds also spontaneously in vitro at 1:1 stoichiometry. The cellular effects of 4-hydroxynonenal evaluated by low-level chemiluminescence and alkane formation are independent of the formation of a glutathione conjugate and seem to rely on the remaining not-bound 4-hydroxynonenal. The sensitivity of 4-hydroxynonenal-enhanced chemiluminescence and alkane formation to free-radical quenchers suggests the participation of a free-radical propagation process.

Cadenas, E; Muller, A; Brigelius, R; Esterbauer, H; Sies, H

1983-01-01

228

Flow injection chemiluminescence determination of hydrazine by oxidation with chlorinated isocyanurates  

Microsoft Academic Search

A rapid and sensitive flow injection chemiluminescence (CL) method is described for the determination of hydrazine based on the CL generated during its reaction with either sodium dichloroisocyanurate (SDCC) or trichloroisocyanuric acid (TCCA) in alkaline medium. The emission intensity is greatly enhanced if dichlorofluorescein (DCF) as sensitizer is present in the reaction medium. The presence of citrate prevents the precipitation

Afsaneh Safavi; Mohammad Ali Karimi

2002-01-01

229

Spontaneous Chemiluminescence of Soybean Embryonic Axes during Imbibition 1  

PubMed Central

Isolated soybean (Glycine max L. var Hood) embryonic axes have a spontaneous chemiluminescence (about 150 counts per minute per embryo) that increases showing two phases, upon water imbibition. The first photoemission burst was measured between 0 and 7 hours of imbibition with a maximum of about 350 counts per minute per embryo after 2 hours. The second photoemission phase, between 7 and 30 hours, increased from about 220 to 520 counts per minute per embryo. Both chemiluminescence phases were inhibited by infused butylated hydroxyanisole while only the second phase was inhibited by infused salicylhydroxamic acid. On the basis of the sensitivity of the lipoxygenase reaction to both inhibitors (about 90%), the first burst is tentatively assigned to oxy-radicals mobilized upon water uptake by the embryonic axes, and the second phase is tentatively identified as due to lipoxygenase activity. The in vivo lipoxygenase activity of the embryonic axes was estimated by both the fraction of total oxygen uptake that was inhibited by butylated hydroxyanisole and by the fraction of photoemission that was inhibited by butylated hydroxyanisole and by salicylhydroxamic acid. Both approaches indicated marked increases (5-fold and 12-fold, respectively) of lipoxygenase activity between 2 and 30 hours of imbibition. The measured chemiluminescence per O2 uptake ratio (the experimental quantum yield) for the lipoxygenase reaction (3.3 × 10?14 counts per O2 molecule) was used to estimate the O2 uptake due to lipoxygenase activity from the photoemission of the embryonic axes after 30 hours of imbibition. The value (0.54 microliters per minute per axis) was close to the butylated hydroxyanisole-sensitive O2 uptake (1.2 microliters O2 per minute per axis) of the same embryonic axes. Chemiluminescence may afford a noninvasive assay for lipoxygenase activity in intact plant tissues.

Boveris, Alberto; Puntarulo, Susana A.; Roy, Adriana H.; Sanchez, Rodolfo A.

1984-01-01

230

Facile chemiluminescent method for alkaline phosphatase determination  

Microsoft Academic Search

The determination of alkaline phosphatase activity is of wide applicability, both as a free enzyme or bound to antibodies (conjugates). Activity determinations employing chemiluminescent substrates have become increasingly important due to their high sensitivity, typically equivalent to or better than assays utilizing radioactive labels. We report here a new chemiluminescent methodology for the determination of alkaline phosphatase activity based on

Valdecir F. Ximenes; Ana Campa; Wilhelm J. Baader; Luiz H. Catalani

1999-01-01

231

Turn-on chemiluminescent sensing platform for label-free protease detection using streptavidin-modified magnetic beads.  

PubMed

We report a label-free streptavidin-modified magnetic beads (SA-MBs)-based sensing platform for turn-on chemiluminescent (CL) detection of protease using trypsin as model analyte. In the assay, a biotinylated peptide containing an arginine and a terminal cysteine was used as the substrate of trypsin. Upon adding the peptide into a basic luminol-NaIO4 solution, the terminal cysteine induced a strong CL signal. Surprisingly a much lower CL was emitted when the peptide was immobilized on the surface of SA-MBs. Based on this phenomenon, we designed a turn-on CL sensing system for protease using trypsin as model and its inhibitors screening. In the absence of trypsin, the peptide was coupled to the SA-MBs surface, resulting in a low CL background. Upon the addition of trypsin, the peptide can be catalytically hydrolyzed at the C-terminus of arginine, resulting in the formation of free cysteine-containing residues and subsequent CL recovery with the addition of luminol and NaIO4. The simple method does not require washing or separating procedures. Trypsin at a concentration as low as 10pM can be assayed using this new CL sensing system. Additionally, the proposed method can be employed for screening the inhibitors of trypsin. This new sensing strategy could be easily extended to assay other proteases by simply changing the peptide substrate. PMID:24846776

Zhang, Huanhuan; Yu, Dalong; Zhao, Yanjun; Fan, Aiping

2014-11-15

232

A novel strategy for synthesis of hollow gold nanosphere and its application in electrogenerated chemiluminescence glucose biosensor.  

PubMed

Well-distributed hollow gold nanospheres (Aushell@GOD) (20±5nm) were synthesized using the glucose oxidase (GOD) cross-linked with glutaraldehyde as a template. A glucose biosensor was prepared based on Aushell@GOD nanospheres for catalyzing luminol electrogenerated chemiluminescence (ECL). Firstly, chitosan was modified in a glassy carbon electrode which offered an interface of abundant amino-groups to assemble Aushell@GOD nanospheres. Then, glucose oxidase was adsorbed on the surface of Aushell@GOD nanospheres via binding interactions between Aushell and amino groups of GOD to construct a glucose biosensor. The Aushell@GOD nanospheres were investigated with TEM and UV-vis. The ECL behaviors of the biosensor were also investigated. Results showed that, the obtained Aushell@GOD nanospheres exhibited excellent catalytic effect towards the ECL of luminol-H2O2 system. The response of the prepared biosensor to glucose was linear with the glucose concentration in the range of 1.0?M to 4.3mM (R=0.9923) with a detection limit of 0.3?M (signal to noise=3). This ECL biosensor exhibited short response time and excellent stability for glucose. At the same time the prepared ECL biosensor showed good reproducibility, sensitivity and selectivity. PMID:25059123

Zhong, Xia; Chai, Ya-Qin; Yuan, Ruo

2014-10-01

233

A critical evaluation of a flow-cell based on a liquid core waveguide for chemiluminescence measurements.  

PubMed

Liquid-core waveguides (LCWs), devices that constrain the emitted radiation minimizing losses during the transport, are an alternative to maximize the amount of detected radiation in luminescence. In this work, the performance of a LCW flow-cell was critically evaluated for chemiluminescence measurements, by using as model the oxidation of luminol by hydrogen peroxide or hypochlorite. An analytical procedure for hypochlorite determination was also developed, with linear response in the range 0.2-3.8 mg/L (2.7-51 micromol/L), a detection limit estimated as 8 microg/L (0.64 micromol/L) at the 99.7% confidence level and luminol consumption of 50 microg/determination. The coefficients of variation were 3.3% and 1.6% for 0.4 and 1.9 mg/L ClO(-), respectively, with a sampling rate of 164 determinations/h. The procedure was applied to the analysis of Dakin's solution samples, yielding results in agreement with those obtained by iodometric titration at the 95% confidence level. PMID:18814188

Sanchez, Mariana A; Rocha, Fábio R P

2008-01-01

234

Light Stick Chemistry: Bioluminescence and Chemiluminescence (title provided or enhanced by cataloger)  

NSDL National Science Digital Library

This activity will offer students an introduction to chemiluminescence and bioluminescence, an opportunity to observe the effect of temperature on reaction, and an activity that integrates art and science. Students explore how the temperature of the chemicals that combine affects a chemiluminescent reaction. They will also learn that since solar radiation does not reach the ocean depths, the bottom of the deep ocean remains largely in complete darkness. However, certain animals in this extreme environment can generate bioluminescent light to communicate with each other and even to lure prey.

Dispezio, Michael

235

Chemiluminescence from bamboo shoot cut.  

PubMed

Bamboo shoot cut emitted weak light, which could be visualized with a two dimensional imaging system. A water extract of bamboo shoot contained tyrosine (a major component of total amino acids), bityrosine and peroxidase. Bamboo shoot peroxidase-H2O2-tyrosine system also emitted weak light with maxima at 490nm, 530nm and longer wavelength, identical to that in horseradish peroxidase-H2O2 system. Judging from these results, chemiluminescence from bamboo shoot cut might be originated from excited species generated by enzymatic oxidation of tyrosine and bityrosine. PMID:8394697

Totsune, H; Nakano, M; Inaba, H

1993-08-16

236

NMR Characterization of Polymers Formed in Diazotizing Mixtures of Luminol and 3-Amino-L-tyrosine.  

National Technical Information Service (NTIS)

A melanin-like polymer was prepared by diazotizing luminol (5-amino-2,3-dihydro-1, 4-phthalazinedione) and 3-amino-L-tyrosine in a mixed water/dimethylsulfoxide solvent; this was followed by acetone precipitation and aging of the solid residue for a month...

J. R. Wright

1993-01-01

237

NMR Characterization of Products Formed in Diazotizing Mixtures of Luminol and 3-Amino-L-Tryosine.  

National Technical Information Service (NTIS)

A diazotized mixture of luminol and 3-amino-L-tyrosine prepared as an acetone-precipitated solid polymerizes slowly (over a period of weeks) yielding a brown, water soluble polymer which migrates electrophoretically as an anion at pH 6. Solutions of this ...

J. R. Wright

1991-01-01

238

Determination of synephrine in weight-loss products using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection  

Microsoft Academic Search

Adrenergic amines found in extracts of Citrus aurantium (bitter orange) evoke analytically useful chemiluminescence with acidic potassium permanganate in the presence of polyphosphates. From corrected chemiluminescence spectra, the wavelength of maximum intensity for these reactions was 680±5nm and, using flow injection analysis methodology, limits of detection for synephrine, octopamine, tyramine and hordenine were found to be between 1×10?9 and 1×10?8M.

Teo Slezak; Paul S. Francis; Nicole Anastos; Neil W. Barnett

2007-01-01

239

Characterization of chemiluminescence from singlet oxygen under laminar flow conditions in a micro-channel and its quenching with beverages  

Microsoft Academic Search

Singlet oxygen was generated by reaction of sodium hypochlorite and hydrogen peroxide in a micro-channel. The two reagent solutions were delivered into the micro-channel by a syringe pump, providing a laminar flow liquid–liquid interface. The chemiluminescence from the singlet oxygen was emitted in the collapse of the interface due to molecular diffusion under laminar flow conditions. The chemiluminescence intensity was

Kazuhiko Tsukagoshi; Kazuaki Fukumoto; Riichiro Nakajima; Kenichi Yamashita; Hideaki Maeda

2007-01-01

240

Speciation of As(III)/As(V) in water samples by a magnetic solid phase extraction based on Fe3O4/Mg-Al layered double hydroxide nano-hybrid followed by chemiluminescence detection.  

PubMed

A novel magnetic solid phase extraction method was developed for the speciation of As(III)/As(V) in aqueous solutions utilizing Fe3O4-doped Mg-Al layered double hydroxide (LDH) as a nano-sorbent. The method is based on the separation and pre-concentration of As(V) by Fe3O4/Mg-Al LDH nano-hybrid prior to determination by a chemiluminescence (CL) technique. The CL route involves the oxidation of luminol by vanadomolybdoarsenate heteropoly acid in a basic media. Since the existing cations cannot be adsorbed by positively charged layers of the LDH and other potentially interferent anions had no considerable effect on the CL reaction, it provides a very selective and sensitive determination approach for As(V). The determination of total arsenic and hence indirectly As(III) involve the pre-oxidation of As(III) to As(V) by a mixture of hydrogen peroxide and potassium hydroxide. Several factors affecting the extraction and determination of the analyte were investigated and optimized. Under optimum conditions, the calibration graph was linear in the range of 5.0-5000ngL(-1). The limit of detection and enrichment factor was 2.0ngL(-1) and 80, respectively. The method was validated by the analysis of a standard reference material (NIST SRM 1643e), and successfully applied to the speciation of arsenic in several water samples with recoveries in the range of 93.3-106.7% for the spiked samples. PMID:25059142

Abdolmohammad-Zadeh, Hossein; Talleb, Zeynab

2014-10-01

241

Development of a cyclic system for chemiluminescence detection.  

PubMed

In this paper, we described a new concept of cyclic chemiluminescence (CCL) detection, and a homemade system was designed to realize such detection. The direction of the carrier in the CCL system is in a state of periodical change that can trigger a succession of chemiluminescence (CL) reactions in a single sample injection. Therefore, in contrast to the traditional CL detection, which only records a single signal, CCL allows us to obtain multistage signals. To evaluate the new method, the cataluminescence (CTL) reaction of the volatile organic compounds (VOCs) on a nanosized catalyst was selected as the analytical model. We found that each CCL reaction has a unique exponential decay equation (EDE) to describe the change law of its multistage signals. Further study showed that the initial amount (A) of the EDE is linear with the analyte concentration, while the decay coefficient (k) is a characteristic constant for a given reaction. The formation mechanism of the exponential function and the determinants of the decay coefficient were discussed in detail. As a distinct application, CCL is capable of rapidly discriminating various analytes and even structural isomers. PMID:24873459

Zhang, Runkun; Hu, Yufei; Li, Gongke

2014-06-17

242

Development of a multichannel flow-through chemiluminescence microarray chip for parallel calibration and detection of pathogenic bacteria.  

PubMed

Pathogen detection is important for health and safety reasons. Several outbreaks all over the world have shown the need for rapid, qualitative, quantitative, and, particularly, multianalyte detection systems. Hence, a multichannel flow-through chemiluminescence microarray chip for parallel detection of pathogenic bacteria was developed. The disposable chip made of acrylonitrile-butadiene-styrene (ABS) copolymer was devised as a support for a multiplexed sandwich immunoassay. Calibration and measurement was possible in one experiment, because the developed chip contains six parallel flow-through microchannels. Polyclonal antibodies against the pathogenic bacteria Escherichia coli O157:H7, Salmonella typhimurium, and Legionella pneumophila were immobilized on the chip by microcontact printing in order to use them as specific receptors. Detection of the captured bacteria was carried out by use of specific detection antibodies labelled with biotin and horseradish peroxidase (HRP)-streptavidine conjugates. The enzyme HRP generates chemiluminescence after adding luminol and hydrogen peroxide. This signal was observed by use of a sensitive CCD camera. The limits of detection are 1.8 x 10(4) cells mL(-1) for E. coli O157:H7, 7.9 x 10(4) cells mL(-1) for L. pneumophila, and 2.0 x 10(7) cells mL(-1) for S. typhimurium. The overall assay time for measurement and calibration is 18 min, enabling very fast analysis. PMID:19575190

Karsunke, Xaver Y Z; Niessner, Reinhard; Seidel, Michael

2009-11-01

243

Analytical Applications of Bioluminescence and Chemiluminescence  

NASA Technical Reports Server (NTRS)

Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized. Photometer designs are also considered.

Chappelle, E. W. (editor); Picciolo, G. L. (editor)

1975-01-01

244

HALOCARBON INTERFERENCES IN CHEMILUMINESCENT MEASUREMENTS OF NOX  

EPA Science Inventory

Anomalous NOx responses were observed when halocarbons were irradiated in the presence of oxides of nitrogen. Interferences to chemiluminescent NOx monitor using heated carbon converter were studied for phosgene, tri-chloroacetyl chloride, chloroform, chlorine, and hydrochloric a...

245

Distributions of instantaneous heat release by the cross-correlation of chemiluminescent emissions  

Microsoft Academic Search

A technique for determining the instantaneous heat release from a localized region of a flame and correlating it with the fluctuating pressure is described. The technique is based on ultraviolet at 307 nm by the chemiluminescence of the OH? radical, which is closely associated with the combustion reactions in gaseous hydrocarbon flames. A novel feature of the approach is the

C. J. Lawn

2000-01-01

246

Storable, thermally activated, near-infrared chemiluminescent dyes and dye-stained microparticles for optical imaging  

PubMed Central

Optical molecular imaging employs relatively harmless, low-energy light and technically straightforward instrumentation. Self-illuminating, chemiluminescent systems are especially attractive since they have inherently high signal contrast due to the lack of background emission. Currently, chemiluminescence imaging involves short-lived molecular species that are not stored but instead generated in situ, and they typically emit visible light, which does not penetrate far through heterogeneous biological media. Here, we describe a new paradigm for optical molecular imaging using squaraine rotaxane endoperoxides (SREPs), interlocked fluorescent and chemiluminescent dye molecules that have a squaraine chromophore encapsulated inside a macrocycle endoperoxide. SREPs can be stored indefinitely at temperatures below ?20 °C, but upon warming to body temperature they undergo a unimolecular chemical reaction and emit near infrared light that can pass through a living mouse. Dye-stained microparticles are easily prepared for in vivo near-infrared optical imaging using commercial imaging stations.

Baumes, Jeffrey M.; Gassensmith, Jeremiah J.; Giblin, Jay; Lee, Jung-Jae; White, Alexander G.; Culligan, William J.; Leevy, W. Matthew; Kuno, Masaru; Smith, Bradley D.

2011-01-01

247

Storable, thermally activated, near-infrared chemiluminescent dyes and dye-stained microparticles for optical imaging  

NASA Astrophysics Data System (ADS)

Imaging techniques are a vital part of clinical diagnostics, biomedical research and nanotechnology. Optical molecular imaging makes use of relatively harmless, low-energy light and technically straightforward instrumentation. Self-illuminating, chemiluminescent systems are particularly attractive because they have inherently high signal contrast due to the lack of background emission. Currently, chemiluminescence imaging involves short-lived molecular species that are not stored but are instead generated in situ, and they typically emit visible light, which does not penetrate far through heterogeneous biological media. Here, we describe a new paradigm for optical molecular imaging using squaraine rotaxane endoperoxides, interlocked fluorescent and chemiluminescent dye molecules that have a squaraine chromophore encapsulated inside a macrocycle endoperoxide. Squaraine rotaxane endoperoxides can be stored indefinitely at temperatures below -20 °C, but upon warming to body temperature they undergo a unimolecular chemical reaction and emit near-infrared light that can pass through a living mouse.

Baumes, Jeffrey M.; Gassensmith, Jeremiah J.; Giblin, Jay; Lee, Jung-Jae; White, Alexander G.; Culligan, William J.; Leevy, W. Matthew; Kuno, Masaru; Smith, Bradley D.

2010-12-01

248

Electrochemiluminescent behavior of luminol on the glassy carbon electrode modified with CoTPP\\/MWNT composite film  

Microsoft Academic Search

A glassy carbon electrode (GCE) modified with cobalt(II) meso-tetraphenylporphrine\\/multiwall-carbon nanotube (CoTPP\\/MWNT) was applied to investigate the electrochemiluminescent (ECL) behavior of luminol. The ECL intensity of luminol was found to be increased greatly on this modified electrode. The presence of cobalt(II) meso-tetraphenylporphrine (CoTPP) can catalyze the reduction of oxygen on the electrode surface to produce HOO?, which can increase the ECL

Zhenyu Lin; Jinhua Chen; Yuwu Chi; Bin Qui; Jinming Lin; Guonan Chen

2008-01-01

249

Multicolor Directional Surface Plasmon-Coupled Chemiluminescence  

PubMed Central

In reports over the past several years, we have demonstrated the efficient collection of optically excited fluorophore emission by its coupling to surface plasmons on thin metallic films, where the coupled luminescence was highly directional and polarized. This phenomenon is referred to as surface plasmon-coupled emission (SPCE). In this current study, we have extended this technique to include chemiluminescing species and subsequentially now report the observation of surface plasmon-coupled chemiluminescence (SPCC), where the luminescence from chemically induced electronic excited states couples to surface plasmons in thin continuous metal films. The SPCC is highly directional and predominantly p-polarized, strongly suggesting that the emission is from surface plasmons instead of the luminophores themselves. This indicates that surface plasmons can be directly excited from chemically induced electronic excited states and excludes the possibility that the plasmons are created by incident excitation light. This phenomenon has been observed for a variety of chemiluminescent species in the visible spectrum, ranging from blue to red, and also on a variety of metals, namely, aluminum, silver, and gold. Our findings suggest new chemiluminescence sensing strategies on the basis of localized, directional, and polarized chemiluminescence detection, especially given the wealth of assays that currently employ chemiluminescence-based detection.

Chowdhury, Mustafa H.; Malyn, Stuart N.; Aslan, Kadir; Lakowicz, Joseph R.; Geddes, Chris D.

2009-01-01

250

Human saliva-based quantitative monitoring of clarithromycin by flow injection chemiluminescence analysis: a pharmacokinetic study.  

PubMed

Human saliva quantitative monitoring of clarithromycin (CLA) by chemiluminescence (CL) with flow injection analysis was proposed for the first time, which was based on the quenching effect of CLA on luminol-bovine serum albumin (BSA) CL system with a linear range from 7.5 × 10(-4) to 2.0 ng/ml. This proposed approach, offering a maximum sample throughput of 100 h(-1), was successfully applied to the quantitative monitoring of CLA levels in human saliva during 24 h after a single oral dose of 250 mg intake, with recoveries of 95.2 ? 109.0% and relative standard deviations lower than 6.5 % (N = 7). Results showed that CLA reached maximum concentration of 2.28 ± 0.02 ?g/ml at approximately 3 h, and the total elimination ratio was 99.6 % in 24 h. The pharmacokinetic parameters including absorption rate constant (0.058 ± 0.006 h(-1)), elimination rate constant (0.149 ± 0.009 h(-1)) and elimination half-life time (4.66 ± 0.08 h) were obtained. A comparison of human saliva and urine monitoring was also given. The mechanism study of BSA-CLA interaction revealed the binding of CLA to BSA is an entropy driven and spontaneous process through hydrophobic interaction, with binding constant K BSA-CLA of 4.78 × 10(6) l/mol and the number of binding sites n of 0.82 by flow injection-chemiluminescence model. Molecular docking analysis further showed CLA might be in subdomain IIA of BSA, with K BSA-CLA of 6.82 × 10(5) l/mol and ?G of -33.28 kJ/mol. PMID:24166104

Tan, Xijuan; Song, Zhenghua

2014-02-01

251

Enhanced electrochemiluminescence from luminol at carboxyl graphene for detection of ?-fetoprotein.  

PubMed

In this study, a novel sensitive electrochemiluminescence (ECL) immunosensor was constructed by carboxyl graphene (GR) for enhancing luminol-O2 system emission. Here, carboxyl GR was used to enhance the ECL intensity of luminol that had excellent electron transfer ability and good solubility. The sensing platform was constructed by depositing carboxyl GR on electrodes and immobilizing antibodies on the surface of carboxyl GR through amidation. The specific immunoreaction between ?-fetoprotein (AFP) and antibodies resulted in a decrease of ECL intensity, and the intensity decreased linearly with AFP concentrations in the range of 5pgml(-1) to 14ngml(-1) with a detection limit of 2.0pgml(-1). The proposed immunosensor exhibits high specificity, good reproducibility, and longtime stability. It may become a promising technique for protein detection. PMID:24769071

Li, Xiaojian; Guo, Qingfang; Cao, Wei; Li, Yueyun; Du, Bin; Wei, Qin

2014-07-15

252

Analysis of prostaglandin G/H synthase-2 inhibition using peroxidase-induced luminol luminescence.  

PubMed

The inducible form of the heme-protein prostaglandin G/H synthase (PGHS-2 or COX-2) has been established as a pivotal enzyme in the cascade of events leading to inflammation, hyperalgesia, and pyresis and represents a major therapeutic target in inflammatory disease. Accordingly, we have exploited the heme-catalyzed hydroperoxidase activity of recombinant hCOX-2 to generate luminescence in the presence of luminol, or a cyclic naphthalene hydrazide, and the substrate arachidonic acid. Arachidonate-induced luminescence was shown to be an index of real-time catalytic activity and demonstrated the turnover inactivation of the enzyme. Luminol luminescence was proportional to hCOX-2 concentration and gave accurate Km determinations for arachidonate. Inhibition of hCOX-2 activity, measured by luminescence, by a variety of selective (for COX-2) and nonselective inhibitors showed rank orders of potency similar to those observed with other in vitro and whole cell methods using the recombinant protein. The sensitivity of the luminescence assay also allowed determination of inhibitor potency at substrate concentrations below Km, distinguishing competitive inhibitors such as ibuprofen from time-dependent inhibitors such as DuP-697. Finally the use of higher quantum-yielding luminol analogues allowed measurement of cyclooxygenase activity at extremely low substrate and protein concentrations, enabling a variety of novel assay formats. PMID:9866686

Forghani, F; Ouellet, M; Keen, S; Percival, M D; Tagari, P

1998-11-15

253

[A comparison of the Bluestar and luminol effectiveness in bloodstain detection].  

PubMed

The objective of the present study was to compare the effectiveness of two chemical agents--Bluestar and luminol--in detection of bloodstains. The experiments were performed to test for bloodstain detection sensitivity, chemical stability and to investigate the effect of both reagents on DNA typing. During this study, the authors prepared serial dilutions (1:2 to 1:10 000 000) of fresh blood, as well as dilutions of 25-year old blood on Whatman 3MM blotting paper. Additional dilutions of fresh blood were spotted on a glass surface. The experiments showed very similar results for both investigated reagents, although the Bluestar solution proved to be more stable (at least 7 days after the preparation) as compared to luminol (stable for not more than 24 hours). Both reagents showed a higher sensitivity for diluted bloodstains on a glass surface than for similar stains on filter paper. The investigators also demonstrated that multiplex amplification of DNA was feasible after Bluestar or luminol treatment, although the detected bloodstains might be too diluted to allow for effective DNA extraction and amplification. PMID:17249372

?uczak, Sylwia; Wo?niak, Marcin; Papuga, Marta; Stopi?iska, Katarzyna; Sliwka, Karol

2006-01-01

254

Ultra-sensitive, high-throughput detection of infectious diarrheal diseases by portable chemiluminescence imaging.  

PubMed

This paper describes a rapid, ultra-sensitive, and high-throughput pathogenic DNA identification strategy for infectious diarrheal diseases diagnosis. This strategy is based on specific DNA hybridization and horseradish-peroxidase-catalyzed chemiluminescence (CL) detection. Probe DNA strands are covalently immobilized on the aldehyde-group-modified slide and hybridized with biotin-modified target DNA strands. Horseradish-peroxidase (HRP) is then combined with the target DNA via a biotin-streptavidin linkage. The subsequently added mixture of luminol and hydrogen peroxide is catalyzed by HRP and radiates photons. The photons are collected and read out by a portable imager. The specific detection of target DNA strands was realized at a detection limitation of about 0.75 nM. This strategy facilitates quantitative detection, as indicated by the fact that the CL signals were consistent well with a linear function. This method was applied to identify a myriad of real diarrheal pathogens samples, including Enterohemorrhagic Escherichia coli (EHEC), Vibrio cholerae (VBC), Shigella (SHLA), and Salmonella (SMLA). Triple-assay of six gene sequences from these pathogens was realized, which facilitates accurate, high-throughput identification of diarrheal pathogens. This CL assay strategy is appropriate for application in disease diagnosis and prevention. PMID:24534578

Wang, Chaoguang; Xiao, Rui; Dong, Peitao; Wu, Xuezhong; Rong, Zhen; Xin, Lin; Tang, Jun; Wang, Shengqi

2014-07-15

255

Fe(III)-TAML activator: A potent peroxidase mimic for chemiluminescent determination of hydrogen peroxide.  

PubMed

Efforts to replace native peroxidase with its low molecular weight alternatives have stimulated a search for peroxidase mimetics. Herein we describe the oxidation of luminol with hydrogen peroxide catalyzed by commercially available Fe(III)-TAML activator 1a, which was shown to be a more active catalyst than hemin. At Fe(III)-TAML activator 1a use in chemiluminescent assay for H2O2 determination the detection limit value (3?) of 5×10(-8)M was similar to the detection limit obtained with horseradish peroxidase (1×10(-7)M) and significantly lower than that obtained in the presence of hemin (6×10(-7)M). The linear ranges (R(2)=0.98) of the assay were 6×10(-8)-1×10(-6)M and 6×10(-7)-1×10(-6)M H2O2 for Fe(III)-TAML 1a and hemin, respectively. The CV values for Fe(III)-TAML 1a-based assay measured within the working range varied from 1.0% to 3.7% (n=4), whereas in the case of hemin -5.0% to 9.7% (n=4). Moreover, the sensitivity of Fe(III)-TAML 1a-based method was 56 and 5 times higher than that of hemin- and HRP-based methods, respectively. The obtained results open good perspectives to apply Fe(III)-TAML activator 1a in CL analytical methods instead of hemin, a traditionally used peroxidase mimetic. PMID:24840457

Vdovenko, Marina M; Demiyanova, Alexandra S; Kopylov, Kirill E; Sakharov, Ivan Yu

2014-07-01

256

Determination of melamine by flow injection analysis based on chemiluminescence system.  

PubMed

In this paper, based upon the phenomenon that melamine can obviously enhance the CL signal of the luminol-H(2)O(2) system in basic medium, a simple, rapid and sensitive flow injection chemiluminescence (FI-CL) method for the determination of melamine has been developed. Under the optimum conditions, the linear range for the determination of melamine was 0.2-80?gmL(-1) with a detection limit of 0.12?gmL(-1) calculated as proposed by IUPAC and a relative standard deviation of 3.26% for 11 solutions of 10?gmL(-1) melamine on the same day. The proposed method was satisfactorily applied to determine melamine in milk-based products and satisfactory results were obtained without interferences from the sample matrix. Moreover, one assay produce takes only 25s and the minimum sampling rate is about 120 samplesh(-1), which indicated that the FI-CL method was suitable for high throughput and real-time melamine analysis. PMID:23140744

Zeng, Hua-Jin; Yang, Ran; Wang, Qing-Wen; Li, Jian-Jun; Qu, Ling-Bo

2011-07-15

257

Microplate analytical method for quinones by pulse photo-irradiation and chemiluminescence detection.  

PubMed

Quinones are widely distributed in nature and have various bioactivities. Besides, quinones are also considered as toxicological intermediates which cause severe dangerous effects. Hereby, a sensitive, simple, and rapid method is reported for quinones determination. The proposed method employed time resolved fluorescence (TRF) microplate reader based chemiluminescent (CL) detection for the first time as a novel approach for measurement. Under pulse photo-irradiation, the unique photochemical characteristic of quinones is exploited to liberate reactive oxygen species (ROS) which reacted with photosensitized CL reagent. L-012, luminol analogue, was selected for its high sensitivity. Under our investigation, para-quinones showed high CL response when compared to ortho-quinones. A linear response was obtained for studied quinone concentrations in the range of 0.05-50 ?M for 1,4-naphthquinone and of 0.05-150 ?M for 2-methyl-1,4-naphthoquinone (menadione) and 9,10-anthraquinone with detection limit (blank + 3SD) of 0.01 ?M. The proposed method allowed the rapid determination of large number of samples in very short time (96 sample/125 s). The proposed method was successfully applied for determination of menadione in spiked human serum. PMID:22910835

Elgawish, Mohamed Saleh; Shimomai, Chikako; Kishikawa, Naoya; Ohyama, Kaname; Nakashima, Kenichiro; Kuroda, Naotaka

2012-10-21

258

Selective Detection of Neurotransmitters by Fluorescence and Chemiluminescence Imaging  

SciTech Connect

In recent years, luminescence imaging has been widely employed in neurochemical analysis. It has a number of advantages for the study of neuronal and other biological cells: (1) a particular molecular species or cellular constituent can be selectively visualized in the presence of a large excess of other species in a heterogeneous environment; (2) low concentration detection limits can be achieved because of the inherent sensitivity associated with fluorescence and chemiluminescence; (3) low excitation intensities can be used so that long-term observation can be realized while the viability of the specimen is preserved; and (4) excellent spatial resolution can be obtained with the light microscope so subcellular compartments can be identified. With good sensitivity, temporal and spatial resolution, the flux of ions and molecules and the distribution and dynamics of intracellular species can be measured in real time with specific luminescence probes, substrates, or with native fluorescence. A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with CCD imaging is down to {micro}M levels of glutamate with reasonable response time. They also found that chemiluminescence associated with the ATP-dependent reaction between luciferase and luciferin can be used to image ATP at levels down to 10 nM in the millisecond time scale. Similar imaging experiments should be feasible in a broad spectrum of biological systems.

Ziqiang Wang; Edward S. Yeung

2001-08-06

259

FLOW INJECTION CHEMILUMINESCENCE DETERMINATION OF SULFIDE BY OXIDATION WITH CHLORINATED ISOCYANURATES  

Microsoft Academic Search

A rapid and sensitive flow injection chemiluminescence (CL) method is described for the determination of sulfide based on the CL generated during its reaction with either sodium dichloroisocyanurate (SDCC) and trichloroisocyanuric acid (TCCA) in alkaline medium. The results of investigation of the sensitized and non-sensitized CL reactions are presented. The emission intensity is greatly enhanced for both SDCC-sulfide and TCCA-sulfide

Afsaneh Safavi; Mohammad Ali Karimi; Mohammad Reza Hormozinezhad

2002-01-01

260

A novel green analytical procedure for monitoring of azoxystrobin in water samples by a flow injection chemiluminescence method with off-line ultrasonic treatment.  

PubMed

A simple and green flow injection chemiluminescence (FI-CL) method for determination of the fungicide azoxystrobin was described for the first time. CL signal was generated when azoxystrobin was injected into a mixed stream of luminol and KMnO4 . The CL signal of azoxystrobin could be greatly improved when an off-line ultrasonic treatment was adopted. Meanwhile, the signal intensity increases with the analyte concentration proportionally. Several variables, such as the ultrasonic parameters, flow rate of reagents, concentrations of sodium hydroxide solution and CL reagents (potassium permanganate, luminol) were investigated, and the optimal CL conditions were obtained. Under optimal conditions, the linear range of 1-100?ng/mL for azoxystrobin was obtained and the detection limit (3?) was determined as 0.13?ng/mL. The relative standard deviation was 1.5% for 10 consecutive measurements of 20?ng/mL azoxystrobin. The method has been applied to the determination of azoxystrobin residues in water samples. PMID:23027659

Yang, Xin-an; Zhang, Wang-bing

2013-01-01

261

Flow-injection post-chemiluminescence method for the determination of palmatine.  

PubMed

A post-chemiluminescence (PCL) phenomenon was observed when palmatine was injected in a mixture of N-bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF) after the chemiluminescence (CL) reaction of NBS-alkaline DCF had finished. Based on the PCL reaction, a rapid and sensitive method for the determination of palmatine was established. Under optimum conditions, the CL intensity was linear, with the concentration of palmatine in the range of 5.0 × 10(-9) to 1.0 × 10(-6) M. The detection limit was 6.0 × 10(-10) M for palmatine. The relative standard deviation for 11 parallel measurements of 1.0 × 10(-7) M palmatine was 1.5%. This method was applied to the determination of palmatine in pharmaceutical samples and biological fluids, with satisfactory results. The possible reaction mechanism is discussed briefly. PMID:23816126

Han, Suqin; Wei, Bei; Hao, Fang

2013-07-01

262

Highly sensitive flow-injection chemiluminescence determination of pyrogallol compounds  

NASA Astrophysics Data System (ADS)

A highly sensitive flow-injection chemiluminescent method for the direct determination of pyrogallol compounds has been developed. Proposed method is based on the enhanced effect of pyrogallol compounds on the chemiluminescence signals of KMnO 4-H 2O 2 system in slightly alkaline medium. Three important pyrogallol compounds, pyrogallic acid, gallic acid and tannic acid, have been detected by this method, and the possible mechanism of the CL reaction is also discussed. The proposed method is simple, convenient, rapid (60 samples h -1), and sensitive, has a linear range of 8 × 10 -10 mol L -1 to 1 × 10 -5 mol L -1, for pyrogallic acid, with a detection limit of 6 × 10 -11 mol L -1, 4 × 10 -8 mol L -1 to 5 × 10 -3 mol L -1 for gallic acid with a detection limit of 9 × 10 -10 mol L -1, and 8 × 10 -8 mol L -1 to 5 × 10 -2 mol L -1 for tannic acid, with a detection limit of 2 × 10 -9 mol L -1, respectively. The relative standard deviation (RSD, n = 15) was 0.8, 1.1 and 1.3% for 5 × 10 -6 mol L -1 pyrogallic acid, gallic acid and tannic acid, respectively. The proposed method was successfully applied to the determination of pyrogallol compounds in tea and coffee samples.

Kanwal, Shamsa; Fu, Xiaohong; Su, Xingguang

2009-12-01

263

Electrogenerated chemiluminescence. 58. Ligand-sensitized electrogenerated chemiluminescence in europium labels.  

PubMed

The electrochemistry and electrogenerated chemiluminescence (ECL) of a series of europium chelates, cryptates, and mixed-ligand chelate/cryptand complexes were studied. The complexes were of the following general forms:? EuL(4)(-), where L = ?-diketonate, a bis-chelating ligand (such as dibenzoylmethide), added as salts (A)EuL(4), where A = tetrabutylammonium ion or piperidinium ion (pipH(+)); Eu(crypt)(3+), where crypt = a cryptand ligand, e.g., 4,7,13,16,21-pentaoxa-1,10-diazabicyclo[8,8,5]tricosane; and Eu(crypt)(L)(2+) for the mixed-ligand systems. ECL was obtained for the chelates and mixed-ligand systems by reducing the complexes at a Pt electrode in the presence of peroxydisulfate in acetonitrile solutions and was attributed to the electron-transfer reaction between the reduced bound ligands and SO(4)(•)(-), followed by intramolecular excitation transfer from the excited ligand orbitals to the metal-centered 4f states. No ECL was observed under the same conditions for the europium complexes incorporating only the cryptand ligands in aqueous solution. The ECL spectra matched the photoluminescence spectra with a narrow emission band observed at 612 nm, corresponding to a metal-centered 4f-4f transition. The ECL efficiencies for the ECL-active species were low, about 10(-)(1)-10(-)(4)% of that of the Ru(bpy)(3)(2+)/S(2)O(8)(2)(-) system under similar conditions. PMID:21619211

Richter, M M; Bard, A J

1996-08-01

264

Experimental and numerical study of chemiluminescent species in low-pressure flames  

NASA Astrophysics Data System (ADS)

Chemiluminescence has been observed since the beginning of spectroscopy, nevertheless, important facts still remain unknown. Especially, reaction pathways leading to chemiluminescent species such as OH?, CH?, C2^{*}, and CO2^{*} are still under debate and cannot be modeled with standard codes for flame simulation. In several cases, even the source species of spectral features observed in flames are unknown. In recent years, there has been renewed interest in chemiluminescence, since it has been shown that this radiation can be used to determine flame parameters such as stoichiometry and heat release under some conditions. In this work, we present a reaction mechanism which predicts the OH?, CH? (in A- and B-state), and C2^{*} emission strength in lean to fuel-rich stoichiometries. Measurements have been performed in a set of low-pressure flames which have already been well characterized by other methods. The flame front is resolved in these measurements, which allows a comparison of shape and position of the observed chemiluminescence with the respective simulated concentrations. To study the effects of varying fuels, methane flame diluted in hydrogen are measured as well. The 14 investigated premixed methane-oxygen-argon and methane-hydrogen-oxygen-argon flames span a wide parameter field of fuel stoichiometry ( ?=0.5 to 1.6) and hydrogen content (H2 vol%=0 to 50). The relative comparison of measured and simulated excited species concentrations shows good agreement. The detailed and reliable modeling for several chemiluminescent species permits correlating heat release with all of these emissions under a large set of flame conditions. It appears from the present study that the normally used product of formaldehyde and OH concentration may be less well suited for such a prediction in the flames under investigation.

Kathrotia, T.; Riedel, U.; Seipel, A.; Moshammer, K.; Brockhinke, A.

2012-06-01

265

On the interaction of luminol with human serum albumin: Nature and thermodynamics of ligand binding  

NASA Astrophysics Data System (ADS)

The mechanism and thermodynamic parameters for the binding of luminol (LH 2) with human serum albumin was explored by steady state and picosecond time-resolved fluorescence spectroscopy. It was shown that out of two possible LH 2 conformers present is solution, only one is accessible for binding with HSA. The thermodynamic parameters like enthalpy (? H) and entropy (? S) change corresponding to the ligand binding process were also estimated by performing the experiment at different temperatures. The ligand replacement experiment with bilirubin confirms that LH 2 binds into the sub-domain IIA of the protein.

Moyon, N. Shaemningwar; Mitra, Sivaprasad

2010-09-01

266

Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore--role of degranulation and myeloperoxidase  

SciTech Connect

Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 microgram/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 micrograms/ml). The additional application of 0.1 microM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the distribution of neutrophils, and these photonic activities were gradually spread to extracellular space. Sodium azide, which prevents myeloperoxidase activity, inhibited Ca2+ ionophore-induced photonic burst from phorbol ester-treated neutrophil. These findings suggest a prerequisite role of degranulation and myeloperoxidase release in luminol-dependent photoemission from stimulated neutrophils.

Suematsu, M.; Oshio, C.; Miura, S.; Suzuki, M.; Houzawa, S.; Tsuchiya, M.

1988-08-30

267

On the quantification of OH*, CH*, and C2* chemiluminescence in flames  

NASA Astrophysics Data System (ADS)

Absolute concentrations of all important chemiluminescent species, OH-A, CH-A, CH-B, and C2-d have been measured for the first time in methane-oxygen flames at low pressure. The optical detection system for chemiluminescence measurements has been calibrated with Rayleigh and Raman scattering of a cw laser, with the latter approach yielding superior results. The measured ratio between the concentration of CH-B and CH-A suggests that the electronically excited CH* is formed close to thermal equilibrium. Introduction of different rate constants for reactions leading to CH-A and CH-B were not necessary to explain the experimental results. Results are compared with a recent numerical model. Deviations in profile shape and peak positions are relatively small for stoichiometric flames, but become more pronounced in richer mixtures. Larger discrepancies are observed for the absolute concentrations, depending on the chemiluminescent species and the stoichiometry. In an attempt to find an alternative method for the quantification of chemiluminescent species, MIR-CRDS has been performed around 3.9 ?m. While H2O and OH-X could be measured, the sensitivity was not high enough to detect the low sub-ppb concentration of OH-A—in part due to the limited reflectivity of mirrors in the MIR, in part due to a significant background of hot H2O lines.

Nau, P.; Krüger, J.; Lackner, A.; Letzgus, M.; Brockhinke, A.

2012-06-01

268

Line scanning system for direct digital chemiluminescence imaging of DNA sequencing blots.  

PubMed

A cryogenically cooled charge-coupled device (CCD) camera equipped with an area CCD array is used in a line scanning system for low-light-level imaging of chemiluminescent DNA sequencing blots. Operating the CCD camera in time-delayed integration (TDI) mode results in continuous data acquisition independent of the length of the CCD array. Scanning is possible with a resolution of 1.4 line pairs/mm at the 50% level of the modulation transfer function. High-sensitivity, low-light-level scanning of chemiluminescent direct-transfer electrophoresis (DTE) DNA sequencing blots is shown. The detection of DNA fragments on the blot involves DNA-DNA hybridization with oligonucleotide-alkaline phosphatase conjugate and 1,2-dioxetane-based chemiluminescence. The width of the scan allows the recording of up to four sequencing reactions (16 lanes) on one scan. The scan speed of 52 cm/h used for the sequencing blots corresponds to a data acquisition rate of 384 pixels/s. The chemiluminescence detection limit on the scanned images is 3.9 x 10(-18) mol of plasmid DNA. A conditional median filter is described to remove spikes caused by cosmic ray events from the CCD images. PMID:8368531

Karger, A E; Weiss, R; Gesteland, R F

1993-07-01

269

Chemiluminescent methods in alcoholic beverage analysis.  

PubMed

Chemiluminescent (CL) techniques employed in alcoholic beverage analysis are summarized. Specific applications to wine, beer, brandy, and tequila are described, and the determination of sulfur compounds, phosphate, L-malate, glycerol, fatty acids, phenolic compounds, and urate is included. Possibilities and limitations of the various CL detection systems are evaluated. PMID:10563870

Navas, M J; Jiménez, A M

1999-01-01

270

Study on a Luminol-based Electrochemiluminescent Sensor for Label-Free DNA Sensing  

PubMed Central

Automatic, inexpensive, simple and sensitive methods for DNA sensing and quantification are highly desirable for biomedical research. The rapid development of both the fundamentals and applications of electrochemiluminescence (ECL) over the past years has demonstrated its potential for analytical and bio-analytical chemistry. This paper reports the quenching effect of DNA on the ECL of luminol and the further development of a DNA sensing device. With the pre-functionalization by a composite of carbon nano-tubes (CNTs) and Au nanoparticles (AuNPs), the sensor provides a novel and valuable label-free approach for DNA sensing. Here the ECL intensity was remarkably decreased when more than 1.0 × 10?12 molar of DNA were adsorbed on the sensor. Linearity of the DNA amount with the reciprocal of ECL intensity was observed. A saturated sensor caused a 92.8% quenching effect. The research also proposes the mechanism for the quenching effect which could be attributed to the interaction between luminol and DNA and the elimination of reactive oxygen species (ROSs) by DNA.

Chu, Hai-Hong; Yan, Ji-Lin; Tu, Yi-Feng

2010-01-01

271

Self-assembly of organogels via new luminol imide derivatives: diverse nanostructures and substituent chain effect  

PubMed Central

Luminol is considered as an efficient sycpstem in electrochemiluminescence (ECL) measurements for the detection of hydrogen peroxide. In this paper, new luminol imide derivatives with different alkyl substituent chains were designed and synthesized. Their gelation behaviors in 26 solvents were tested as novel low molecular mass organic gelators. It was shown that the length and number of alkyl substituent chains linked to a benzene ring in gelators played a crucial role in the gelation behavior of all compounds in various organic solvents. Longer alkyl chains in molecular skeletons in present gelators are favorable for the gelation of organic solvents. Scanning electron microscope and atomic force microscope observations revealed that the gelator molecules self-assemble into different micro/nanoscale aggregates from a dot, flower, belt, rod, and lamella to wrinkle with change of solvents. Spectral studies indicated that there existed different H-bond formations and hydrophobic forces, depending on the alkyl substituent chains in molecular skeletons. The present work may give some insight to the design and characteristic of new versatile soft materials and potential ECL biosensors with special molecular structures.

2013-01-01

272

Quenching effect of some heavy metal ions on the fast peroxyoxalate-chemiluminescence of 1-(dansylamidopropyl)-1-aza-4,7,10-trithiacyclododecane as a novel fluorophore  

NASA Astrophysics Data System (ADS)

The fast chemiluminescence (CL) arising from the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with hydrogen peroxide in the presence of 1-(dansylamidopropyl)-1-aza-4,7,10-trithiacyclododecane ( L) as a novel fluorophore, and imidazole as catalyst, has been studied in ethyl acetate solution. The relationships between the chemiluminescence intensity and concentrations of TCPO, imidazole, hydrogen peroxide and L are reported. In the presence of imidazole as catalyst, the entire CL signal was completed in less than 3 s. The quenching effect of Cu 2+, Pb 2+, Cd 2+, Hg 2+ and Ag + ions on the chemiluminescent system was investigated, the resulting Stern-Volmer plots were obtained and the KQ values were calculated. It was found that the quenching effect of metal ions on the chemiluminescence of L decreases in the order Cu 2+ > Pb 2+ > Cd 2+ > Hg 2+ > Ag +.

Shamsipur, Mojtaba; Zargoosh, Kiomars; Hosseini, Seyed Morteza; Caltagirone, Claudia; Lippolis, Vito

2009-09-01

273

Chemiluminescence assay for the sensitive detection of iodide based on extracting Hg2+ from a T-Hg(2+)-T complex.  

PubMed

A simple and sensitive chemiluminescence assay for iodide (I(-)) detection was reported, which was based on iodide extracting Hg(2+) from DNA featuring a stem-loop structure containing T-Hg(2+)-T. Specifically, Hg(2+) induced random coiled G-rich single-strand DNA to form a stem-loop structure containing T-Hg(2+)-T. Because the binding of Hg(2+) and I(-) is much stronger than that of Hg(2+) and thymine (T), I(-) could extract Hg(2+) from the stem-loop structure, releasing the DNA, which then bound with K(+) and transformed into a K(+)-stabilized G-quadruplex (with hemin as a cofactor), which catalyzed the H(2)O(2)-mediated oxidation of luminol. The produced chemiluminescence as a sensing signal was applied to sensitively and selectively detect iodide with a detection limit of 12 nM. This system exhibited the first DNAzyme-based iodide sensor. Finally, the sensor was successfully applied for iodide detection in real lake water samples. PMID:23391999

Li, Tao; Liang, Gang; Li, Xiaohong

2013-03-21

274

Determination of synephrine in weight-loss products using high performance liquid chromatography with acidic potassium permanganate chemiluminescence detection.  

PubMed

Adrenergic amines found in extracts of Citrus aurantium (bitter orange) evoke analytically useful chemiluminescence with acidic potassium permanganate in the presence of polyphosphates. From corrected chemiluminescence spectra, the wavelength of maximum intensity for these reactions was 680+/-5 nm and, using flow injection analysis methodology, limits of detection for synephrine, octopamine, tyramine and hordenine were found to be between 1x10(-9) and 1x10(-8) M. We have applied this method of detection to the rapid determination of synephrine in dietary supplements using monolithic column chromatography. PMID:17531829

Slezak, Teo; Francis, Paul S; Anastos, Nicole; Barnett, Neil W

2007-06-12

275

New method for the photo-chemiluminometric determination of benzoylurea insecticides based on acetonitrile chemiluminescence.  

PubMed

The viability of tandem photochemical reaction-chemiluminescence detection has been studied for the determination of five benzoylurea insecticides, namely, diflubenzuron, triflumuron, hexaflumuron, lufenuron and flufenoxuron. The 'on-line' photochemical reaction of benzoylurea pesticides provides an enhanced chemiluminescence response of the pesticides during their oxidation by potassium hexacyanoferrate(III) and sodium hydroxide, whose signal increases with the percentage of acetonitrile in the reaction medium. The determination was performed using a photoreactor consisting of a PFA (perfluoroalkoxy) tube reactor coil (5 mx1.6-mm O.D. and 0.8-mm I.D.) and an 8-W xenon lamp. As the yield of the photoderivatization process and the chemiluminescent signals depend on the percentage of acetonitrile, the chromatographic column (a Gemini C18, Phenomenex 150 mmx4.6 mm, 5-microm particle size) was chosen with the aim of using high percentages of this organic solvent in the mobile phase. Previous studies showed that the rate of the chemiluminescent reaction was very fast. Therefore, a modification was carried out in the detector in order to mix the analytes and reactants as near as possible to the measure cell. The optimised method was validated with respect to linearity, precision, limits of detection and quantification accuracy. Under the optimised conditions, linear working range extends three orders of magnitude with the relative standard deviation of intra-day precision below 10% and detection limits between 0.012 and 0.18 microg mL-1, according to the compound. The proposed method has been successfully applied to the determination of benzoylureas in cucumber with good results. PMID:17205265

Gil García, M D; Martínez Galera, M; Santiago Valverde, R

2007-03-01

276

Advances in the use of acidic potassium permanganate as a chemiluminescence reagent: a review.  

PubMed

We review the analytical applications of acidic potassium permanganate chemiluminescence published since our previous comprehensive review in mid-2007 to early 2013. This includes a critical evaluation of evidence for the emitting species, the influence of additives such as polyphosphates, formaldehyde, sulfite, thiosulfate, lanthanide complexes and nanoparticles, the development of a generalized reaction mechanism, and the use of this chemistry in pharmaceutical, clinical, forensic, food science, agricultural and environmental applications. PMID:24356216

Adcock, Jacqui L; Barnett, Neil W; Barrow, Colin J; Francis, Paul S

2014-01-01

277

Simultaneous determination of rifampicin and isoniazid by continuous-flow chemiluminescence with artificial neural network calibration  

Microsoft Academic Search

In this paper a continuous-flow chemiluminescence (CL) system with artificial neural network calibration is proposed for simultaneous\\u000a determination of rifampicin and isoniazid. This method is based on the different kinetic spectra of the analytes in their\\u000a CL reaction with alkaline N-bromosuccinimide as oxidant. The CL intensity was measured and recorded every second from 1 to 300 s. The data obtained were

Baoxin Li; Yuezhen He; Jiagen Lv; Zhujun Zhang

2005-01-01

278

Xanthene dye chemiluminescence for determination of free chlorine in water  

SciTech Connect

Preliminary investigations by a batch method are described for aiming at the flow determination of free chlorine in water with novel chemiluminescence (CL) detection. The CL originates from the reaction of xanthene dyes with free chlorine, Cl/sub 2/, HOCl, and OCl/sup -/. Through the measurements of CL decay curves, fundamental CL characteristics were explored from the analytical point of view. Among xanthene dyes tested, eosin Y, eosin B, pyronin B, and rhodamine 6G were found to be promising CL reagents with such sensitivity and selectivity that free chlorine can be readily determined in tap water. In particular, these CL systems have the special advantage of being insensitive to oxo acids of chlorine and chloramine. Recommended flow systems are proposed.

Yamada, M.; Hobo, T.; Suzuki, S.

1988-10-01

279

Chemiluminescence determination of ferulic acid by flow-injection analysis using cerium(IV) sensitized by rhodamine 6G  

NASA Astrophysics Data System (ADS)

A simple, sensitive and rapid flow-injection chemiluminescence method has been developed for the determination of ferulic acid based on the chemiluminescence reaction of ferulic acid with rhodamine 6G and ceric sulfate in sulphuric acid medium. Strong chemiluminescence signal was observed when ferulic acid was injected into the acidic ceric sulfate solution in a flow-cell. The present method allowed the determination of ferulic acid in the concentration range of 8.0 × 10 -6 to 1.0 × 10 -4 mol l -1 and the detection limit for ferulic acid was 8.7 × 10 -9 mol l -1. The relative standard deviation was 2.4% for 10 replicate analyses of 1.0 × 10 -5 mol l -1 ferulic acid. The proposed method was applied to the determination of ferulic acid in Taita Beauty Essence samples with satisfactory results.

Wang, Ju Peng; Li, Nian Bing; Luo, Hong Qun

2008-11-01

280

Ionization and chemiluminescence during the progressive aeration of methane flames  

SciTech Connect

Saturation currents and chemiluminescence, especially at the CH{sup *} and C{sub 2}{sup *} wavelengths, are measured for a range of small, laminar methane flames during progressive addition of air, with the principal objective of distinguishing between pure diffusion flames, premixed flames of compositions falling between the upper and lower flammability limits, and the broad range of aerated flames lying in between these regimes. Flame areas defined by the loci of maximum luminosity and by schlieren contours were recorded, so that saturation current densities, CH{sup *} and C{sub 2}{sup *} emission per unit flame area, as well as burning velocities could be deduced. For admixtures of less than 70 vol.%, air appears to act, surprisingly, as an inert diluent as regards saturation currents, so that saturation currents are essentially proportional to fuel flow alone. Much the same applies to chemiluminescence. However, schlieren contours, which were recorded both to provide a basis for burning velocity measurements and to explore density changes in the reactants, indicated the presence of a burner - stabilised propagating reaction zone ahead of the luminous flame surface starting at around 50 vol.% and possibly even at lower air admixtures. This evidence of a steep change in refractive index is indicative of a premixed reaction zone involving the added oxygen, which however generates no chemi-ionization and emits no light. Even photographing the flame by radiation emitted at the CH{sup *} and C{sub 2}{sup *} wavelengths shows no sign of its existence. Its burning velocity is about 10 cm/s, when stabilized by the surrounding diffusion flame. The most plausible rationale for these observations is the formation of syngas by the partial oxidation of methane. The subsequent burning of CO and H{sub 2} is known to occur without chemi-ionization or appreciable light emission. (author)

Weinberg, Felix; Carleton, Fred [Imperial College London, London SW7 2AZ (United Kingdom)

2009-12-15

281

Internal detection of surface plasmon coupled chemiluminescence during chlorination of potassium thin films.  

PubMed

The interaction of chlorine with potassium surfaces is a prototype reaction with a strong non-adiabatic energy transfer leading to exoemission and chemiluminescence. Thin film K/Ag/p-Si(111) Schottky diodes with 8 nm potassium on a 5-200 nm thick Ag layer are used as 2?-photodetectors for the chemiluminescence during chlorination of the K film at 110 K. The observed photocurrent shows a sharp maximum for small exposures and decreases gradually with the increasing chloride layer. The time dependence can be explained by the reaction kinetics, which is governed initially by second-order adsorption processes followed by an electric field-assisted diffusion. The detector current corresponds to a yield of a few percent of elementary charge per reacting chlorine molecule and is orders of magnitude larger than for external detection. The photoyield can be enhanced by increasing the Ag film thickness. For Ag films of 30 and 50 nm, the yield exhibits a maximum indicating surface plasmon coupled chemiluminescence. Surface plasmon polaritons in the Ag layer are excited by the reaction and decay radiatively into Si leading to the observed currents. A model calculation for the reverse process in attenuated total reflection is applied to explain the observed current yield maxima. PMID:23343296

Becker, Felix; Krix, David; Hagemann, Ulrich; Nienhaus, Hermann

2013-01-21

282

Comparison of the effects of antioxidant non-steroidal anti-inflammatory drugs against myeloperoxidase and hypochlorous acid luminol-enhanced chemiluminescence  

Microsoft Academic Search

The interaction of myeloperoxidase (MPO) with H2O2 and Cl? provides a potent antimicrobial\\/cytotoxic system for polymorphonuclear leukocytes (PMNs). MPO-related cytotoxicity may be associated with the formation of toxic oxidant MPO intermediates, HOCl, or both. MPO itself is able to oxidize drugs and cellular components. Non-steroidal anti-inflammatory drugs (NSAIDs) able to act as antioxidant free radical scavengers have recently been shown

Gary Pekoe; Knox Van Dyke; Henry Mengoli; David Peden; Denis English

1982-01-01

283

Intensification of electrochemiluminescence of luminol on TiO2 supported Au atomic cluster nano-hybrid modified electrode.  

PubMed

With TiO(2) nanoparticles as carrier, a supported nano-material of Au atomic cluster/TiO(2) nano-hybrid was synthesized. It was then modified onto the surface of indium tin oxide (ITO) by Nafion to act as a working electrode for exciting the electrochemiluminescence (ECL) of luminol. The properties of the nano-hybrid and the modified electrode were characterized by XRD, XPS, electronic microscopy, electrochemistry and spectroscopy. The experimental results demonstrated that the modification of this nano-hybrid onto the ITO electrode efficiently intensified the ECL of luminol. It was also revealed that the ECL intensity of luminol on this modified electrode showed very sensitive responses to oxygen and hydrogen peroxide. The detection limits for dissolved oxygen and hydrogen peroxide were 2 ?g L(-1) and 5.5 × 10(-12) M, respectively. Besides the discussion of the intensifying mechanism of this nano-hybrid for ECL of luminol, the developed method was also applied for monitoring dissolved oxygen and evaluating the scavenging efficiency of reactive oxygen species of the Ganoderma lucidum spore. PMID:22382937

Yu, Zhimin; Wei, Xiuhua; Yan, Jilin; Tu, Yifeng

2012-04-21

284

First Observation of Surface Plasmon-Coupled Chemiluminescence (SPCC)  

PubMed Central

In this letter, we report the first observation of surface plasmon-coupled chemiluminescence (SPCC), where the luminescence from chemically induced electronic excited states couples to surface plasmons in a thin continuous silver film. The SPCC is highly directional and predominantly p-polarized, strongly suggesting that the emission is from surface plasmons instead of the luminophores directly themselves. This indicates that surface plasmons can be directly excited from chemically induced excited states. With a wealth of assays that employ chemiluminescence based detection currently in use, then our findings suggest new chemiluminescence sensing strategies based on localized, directional and polarized chemiluminescence detection.

Chowdhury, Mustafa H.; Malyn, Stuart N.; Aslan, Kadir; Lakowicz, Joseph R.; Geddes, Chris D.

2007-01-01

285

Enhanced electrogenerated chemiluminescence in thermoresponsive microgels.  

PubMed

The electrochemistry, photoluminescence and electrogenerated chemiluminescence of thermoresponsive redox microgels were investigated. For the first time, reversible ECL enhancement is demonstrated in stimuli-responsive 100-nm microgel particles. Such an unexpected amplification reached 2 orders of magnitude, and it is intrinsically correlated with the collapse of the microgel particles. The swell-collapse transition decreases the average distance between adjacent redox sites and favors the electron-transfer processes in the microgels resulting in the enhanced ECL emission. PMID:23540773

Pinaud, Florent; Russo, Lorenzo; Pinet, Sandra; Gosse, Isabelle; Ravaine, Valérie; Sojic, Neso

2013-04-17

286

Hot Electron-Induced Electrogenerated Chemiluminescence  

Microsoft Academic Search

\\u000a In this chapter, we discuss the basics of cathodic hot electron-induced electrogenerated chemiluminescence (HECL). In the\\u000a applications of HECL, we discuss, e.g., the usable electrode materials and their advantages as well as the applicable solution\\u000a conditions in aqueous media. We also summarize the luminophore types excitable by this method and their usability as labels\\u000a in practical bioaffinity assay applications.

Johanna Suomi; Sakari Kulmala

287

Assessment of Antioxidant Activity of Spray Dried Extracts of Psidium guajava Leaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils  

PubMed Central

This work evaluated the physicochemical properties and antioxidant activity of spray dried extracts (SDE) from Psidium guajava L. leaves. Different drying carriers, namely, maltodextrin, colloidal silicon dioxide, Arabic gum, and ?-cyclodextrin at concentrations of 40 and 80% relative to solids content, were added to drying composition. SDE were characterized through determination of the total phenolic, tannins, and flavonoid content. Antioxidant potential of the SDE was assessed by two assays: cellular test that measures the luminol-enhanced chemiluminescence (LumCL) produced by neutrophils stimulated with phorbol myristate acetate (PMA) and the DPPH radical scavenging (DPPH? method). In both assays the antioxidant activity of the SDE occurred in a concentration-dependent manner and showed no toxicity to the cells. Using the CLlum method, the IC50 ranged from 5.42 to 6.50?µg/mL. The IC50 of the SDE ranged from 7.96 to 8.11?µg/mL using the DPPH• method. Psidium guajava SDE presented significant antioxidant activity; thus they show high potential as an active phytopharmaceutical ingredient. Our findings in human neutrophils are pharmacologically relevant since they indicate that P. guajava SDE is a potential antioxidant and anti-inflammatory agent in human cells.

Fernandes, M. R. V.; Azzolini, A. E. C. S.; Martinez, M. L. L.; Souza, C. R. F.; Lucisano-Valim, Y. M.; Oliveira, W. P.

2014-01-01

288

Assessment of Antioxidant Activity of Spray Dried Extracts of Psidium guajava Leaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils.  

PubMed

This work evaluated the physicochemical properties and antioxidant activity of spray dried extracts (SDE) from Psidium guajava L. leaves. Different drying carriers, namely, maltodextrin, colloidal silicon dioxide, Arabic gum, and ? -cyclodextrin at concentrations of 40 and 80% relative to solids content, were added to drying composition. SDE were characterized through determination of the total phenolic, tannins, and flavonoid content. Antioxidant potential of the SDE was assessed by two assays: cellular test that measures the luminol-enhanced chemiluminescence (LumCL) produced by neutrophils stimulated with phorbol myristate acetate (PMA) and the DPPH radical scavenging (DPPH? method). In both assays the antioxidant activity of the SDE occurred in a concentration-dependent manner and showed no toxicity to the cells. Using the CLlum method, the IC50 ranged from 5.42 to 6.50?µg/mL. The IC50 of the SDE ranged from 7.96 to 8.11?µg/mL using the DPPH(•) method. Psidium guajava SDE presented significant antioxidant activity; thus they show high potential as an active phytopharmaceutical ingredient. Our findings in human neutrophils are pharmacologically relevant since they indicate that P. guajava SDE is a potential antioxidant and anti-inflammatory agent in human cells. PMID:24822200

Fernandes, M R V; Azzolini, A E C S; Martinez, M L L; Souza, C R F; Lucisano-Valim, Y M; Oliveira, W P

2014-01-01

289

Flow injection-chemiluminescence determination of dopamine using potassium permanganate and formaldehyde system.  

PubMed

A simple and sensitive flow injection-chemiluminescence method for the determination of dopamine has been proposed. The method is based on the enhancing effect of dopamine on the chemiluminescence emission generated by the reaction of potassium permanganate with formaldehyde in an acidic medium. The proposed procedure allows the determination of dopamine over the concentration range of 3.1×10(-8)-1.7×10(-5) mol/L and with a detection limit of 1.0×10(-8) mol/L. The linear regression equation was F=44.4912+1.07×10(9)(*)C (correlation coefficient, r(2)=0.9998). The relative standard deviation is 2.1% for the determination of 1.0×10(-8) mol/L dopamine (n=11). The method was successfully applied to the determination of dopamine in pharmaceutical preparation with satisfactory results. The recoveries were found in the range of 96.5-101.3%. PMID:22683557

Wabaidur, Saikh Mohammad; Alothman, Zeid Abdullah; Alam, Seikh Mafiz; Lee, Sang Hak

2012-10-01

290

Chemiluminescent Nanomicelles for Imaging Hydrogen Peroxide and Self-Therapy in Photodynamic Therapy  

PubMed Central

Hydrogen peroxide is a signal molecule of the tumor, and its overproduction makes a higher concentration in tumor tissue compared to normal tissue. Based on the fact that peroxalates can make chemiluminescence with a high efficiency in the presence of hydrogen peroxide, we developed nanomicelles composed of peroxalate ester oligomers and fluorescent dyes, called peroxalate nanomicelles (POMs), which could image hydrogen peroxide with high sensitivity and stability. The potential application of the POMs in photodynamic therapy (PDT) for cancer was also investigated. It was found that the PDT-drug-loaded POMs were sensitive to hydrogen peroxide, and the PDT drug could be stimulated by the chemiluminescence from the reaction between POMs and hydrogen peroxide, which carried on a self-therapy of the tumor without the additional laser light resource.

Chen, Rui; Zhang, Luzhong; Gao, Jian; Wu, Wei; Hu, Yong; Jiang, Xiqun

2011-01-01

291

Air analysis: determination of hydrogen peroxide by chemiluminescence  

NASA Astrophysics Data System (ADS)

The purpose of the present review is to examine the application of chemiluminescence for H 2O 2 in air analysis. The revised literature covers the papers of analytical interest which have appeared in the past few years. Practical considerations, requirements and methodologies are described in order to emphasize the scope and possibilities of chemiluminescence in this area.

Navas, M. J.; Jiménez, A. M.; Galán, G.

292

Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazides by 6-hydroxybenzothiazoles.  

PubMed

6-Hydroxybenzothiazole, 2-cyano-6-hydroxybenzothiazole, and 2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid (dehydroluciferin) dramatically enhance light emission from the horseradish peroxidase conjugate catalyzed oxidation of luminol, isoluminol, N-(6-aminobutyl)-N-ethyl isoluminol, and 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide by either peroxide or perborate. Light emission is enhanced by up to 1000-fold, which is an improvement over the enhancement previously observed using firefly luciferin (4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid). Enhancement is influenced by enhancer concentration and pH. Spectral scans of light emitted in enhanced and unenhanced reactions are similar, suggesting that aminophthalate products, and not the enhancers, are the emitters. PMID:4003766

Thorpe, G H; Kricka, L J; Gillespie, E; Moseley, S; Amess, R; Baggett, N; Whitehead, T P

1985-02-15

293

Revisiting flow-chemiluminescence techniques: pharmaceutical analysis.  

PubMed

The state of the art in flow-chemiluminescence (flow-CL) technique for automated pharmaceutical analysis is reviewed. Flow-CL approaches have become powerful and promising tools for pharmaceutical screening in recent years due to their simplicity, low cost and high sensitivity. Because of these advantages, these methods have been widely used for pharmaceutical analysis in recent years. The literature reviewed covers papers of analytical interest that appeared between 2007 and mid-2012 and have been divided into several sections based on fundamental types of CL systems employed. Furthermore, entries have been summarized alphabetically in tabular form giving details of analytical figures of merit of the methods. PMID:23124900

Iranifam, Mortaza

2013-01-01

294

Molecular Fluorescence, Phosphorescence, and Chemiluminescence Spectrometry  

SciTech Connect

This review covers the 2 year period since our last review (1) from January 2008 through December 2009. A computer search of Chemical Abstracts provided most of the references for this review. A search for documents written in English containing the terms fluorescence or phosphorescence or chemiluminescence published in 2008-2009 resulted in more than 100 000 hits. An initial screening reduced this number to approximately 23 000 publications that were considered for inclusion in this review. Key word searches of this subset provided subtopics of manageable size. Other citations were found through individual searches by the various authors who wrote a particular section of this review.

Powe, Aleeta [University of Louisville, Louisville; Das, Susmita [Louisiana State University; Lowry, Mark [Lousianna State University; El-Zahab, Bilal [Lousianna State University; Fakayode, Sayo [Winston-Salem State University; Geng, Maxwell [University of Iowa; Baker, Gary A [ORNL; Wang, Lin [Southern Illinois University; McCarroll, Matthew [Southern Illinois University; Patonay, Gabor [Georgia State University, Atlanta; Li, Min [Lousianna State University; Aljarrah, Mohannad [Louisiana State University; Neal, Sharon [University of Delaware; Warner, Isiah M [ORNL

2010-01-01

295

Real-time monitoring of singlet oxygen in photodynamic therapy with chemiluminescence  

NASA Astrophysics Data System (ADS)

Photodynamic therapy (PDT) is a cancer therapy most of which using light excites sensitizer mainly to produce singlet oxygen (1O2) to kill tumor cells by oxidation reaction. Monitoring the singlet oxygen production is an important task for getting more useful dosage information in photodynamic therapy to enhance the effect. In order to monitor singlet oxygen in PDT, the Chemiluminescence (CL) probe, which could react with singlet oxygen and emit photons, was selected and employed on mice to produce CL. The CL was collected and recorded by a single photon detection system in real time. The results showed that the signal intensity was high and indicated that the chemiluminescence could measure singlet oxygen in vivo sensitively. And during photodynamic therapy the CL signal dropped gradually. Different therapy dosages had different decay life. Any of the decay had two different parts: the rapid component and the slow component. During PDT, reactive oxygen would oxidize biomolecules of tissue, and oxygen was consumed. It would cause a rapid component; by combining with chemiluminescence and fluorescence detection technique, the first-order elimination coefficient of tissue was proved to be degressive during PDT. We deduced that the damaged vascular in PDT would provide littler oxygen and tissue hypoxia was more severely. It may quicken CL decay and caused the slow component. In conclusion, the results proved that monitoring 1O2 by CL could give useful information not only to evaluate the effect of PDT but also to judge the tissue oxygen depletion.

Wei, Yan Chun; Yang, Li Yong; Song, Jia Xing

2008-12-01

296

Chemiluminescence of BO{sub 2} to map the creation of thermal NO in flames  

SciTech Connect

The aim of this study is to detect and map the local conditions that generate thermal NO in flames. According to the Zeldovich mechanism, the formation of NO comes from the local conjunction of a high concentration of atomic oxygen and a temperature above a critical high level imposed by the high activation energy of the rate-limiting reaction. The green light emitted when a flame is seeded with boron salts is a chemiluminescence from the BO{sup *}{sub 2} that is chemically formed in its excited state when BO reacts with atomic oxygen. As the rate of this oxidation is also strongly increasing with temperature, the chemiluminescence of BO{sub 2} depends on the concentration of atomic oxygen and on the temperature in a way similar to the formation rate of thermal NO. This double analogy suggests the possibility of an experimental in situ simulation of the formation rate of thermal NO or at least the use of the chemiluminescence of BO{sub 2} to map the sites where thermal NO is being created. Spectroscopic experiments and comparisons with numerical simulations have been performed to test the feasibility of this technique in laminar premixed and diffusion methane/air flames. The agreement is good except in the burnt gases of fuel-rich flames. Imaging strategies with different spectral filters have been developed in the same flames to overcome the problem of interference from soot radiation in diffusion flames. (author)

Maligne, D. [CORIA CNRS UMR6614, Site Universitaire du Madrillet, BP12, 76801 St Etienne du Rouvray Cedex (France)]|[Renault s.a.s., 1 allee Cornuel, 91510 Lardy (France)]|[IFP, 1 et 4 avenue de Bois-Preau, 92852 Rueil-Malmaison Cedex (France); Cessou, A.; Stepowski, D. [CORIA CNRS UMR6614, Site Universitaire du Madrillet, BP12, 76801 St Etienne du Rouvray Cedex (France)

2009-02-15

297

Menadione-catalyzed luminol luminescent assay as a novel evaluation method of ethanol tolerance in yeast cells  

Microsoft Academic Search

In this study, ethanol inhibited the growth and glucose-induced proton release of yeast cells in a dose-dependent manner. On the other hand, ethanol tolerance of menadione-catalyzed luminol luminescence by yeast cells increased with increasing ethanol concentrations in the growth medium. The intracellular reduced-form nicotinamide adenine dinucleotide (NADH) concentration also increased with increasing ethanol concentrations in the medium and was enough

Shiro Yamashoji

2009-01-01

298

Measurement and simulation of rotationally-resolved chemiluminescence spectra in flames  

NASA Astrophysics Data System (ADS)

In recent years, there has been renewed interest in chemiluminescence, since it has been shown that these emissions can be used to determine flame parameters such as stoichiometry and heat release under some conditions. Even though the origin of these emissions has been known for a long time, little attention has been paid to the detailed analysis of the spectral structure. In this contribution, we present rotationally-resolved spectra of all important chemiluminescent emissions OH A-X, CH B-X, CH A-X, and C2 d-a in CH4/air flames. A numerical model based on the LASKIN ? 2 code has been developed that allows, for the first time, to accurately predict the shape of the measured spectra for all of these transitions. Reabsorption of chemiluminescence within the emitting flame is shown to be a major factor, affecting both intensity and structure of OH? spectra. Even in lab-scale flames, it might change the intensity of individual lines by a factor of 5. The shape of chemiluminescence spectra depends on several processes including initial state distribution and rotational and vibrational energy transfer (which, in turn, depend on the collisional environment and the temperature). It is shown that chemical reactions form OH? in highly excited states and that the number of collisions is not sufficient to equilibrate the initial distribution. Therefore, high apparent temperatures are necessary to describe the shape of the measured spectra. In contrast, CH? is formed with less excess energy and the spectral shape is very close to thermal. The rotational structure of C2^{*} is close to thermal equilibrium as well. Vibrational temperatures are, however, significantly higher than the flame temperature. Implications and perspectives for flame measurements are discussed.

Brockhinke, A.; Krüger, J.; Heusing, M.; Letzgus, M.

2012-06-01

299

Intensification of the electrochemiluminescence of luminol on hollow TiO2 nanoshell-modified indium tin oxide electrodes.  

PubMed

Hollow titania nanoshells (HTNSs), which were synthesized by a SiO2 sacrificial template method, were used to intensify the electrochemiluminescence (ECL) of luminol. The size, shell thickness and crystal phase, factors that are important in determining the efficiency, can be controlled by adjusting the template size, precursor concentration and calcination temperature, respectively. The structure of the HTNSs was characterized by transmission electron microscopy, scanning electron microscopy and X-ray diffraction spectroscopy. After structural optimization, the surface of indium tin oxide (ITO)-coated glass was modified with the HTNSs to act as a working electrode for a flow-injection analytical system. The heterostructure demonstrated an ECL emission intensity 150 times higher than that of the bare ITO. The research also revealed that the ECL of luminol on this modified electrode showed a very sensitive response to hydrogen peroxide with a detection limit of 4.6×10(-10)M. In addition to discussing the intensifying mechanism of luminol ECL by HTNSs, we demonstrate that can be successfully applied to evaluate the gross antioxidant activity of garlic. PMID:25059155

Hong, Jia; Ming, Liang; Tu, Yifeng

2014-10-01

300

Chemiluminescence detection of proteins from single cells.  

PubMed Central

The analysis of proteins from single cells requires techniques of supreme sensitivity. Although radiochemical procedures are capable of detecting small amounts of electrophoretically separated proteins, their sensitivity falls short of that required for routine detection of minor components of single cells. Utilizing the avidin-biotin interaction and the alkaline phosphatase substrate 3-(4-methoxyspiro[1,2-dioxetane-3,2'- tricyclo-[3.3.1.1(3,7)]decan]-4-yl)phenyl phosphate (AMPPD), we have developed an alternative, chemiluminescence-based method for protein detection whose sensitivity exceeds that of other methods. Applying this method to a purified protein, we could detect as little as 63 fg (0.9 amol) of biotinylated bovine serum albumin. The sensitivity of the method was demonstrated by the detection of proteins from individual photoreceptor outer segments, including proteins constituting approximately 1% of the total. Chemiluminescence detection also proved extremely sensitive for immunoblotting: a comparison of five methods for detection of antibody-antigen interactions showed that the AMPPD technique was more sensitive than detection with a colorimetric alkaline phosphatase substrate, 125I-labeled protein A, 125I-labeled anti-mouse IgG, or colloidal gold-conjugated anti-mouse IgG. Images

Gillespie, P G; Hudspeth, A J

1991-01-01

301

Micromachined chemiluminescent system for explosives detection  

NASA Astrophysics Data System (ADS)

Under a recent US Office of Naval Research University Affiliated Research Center (UARC) Basic Research Program we have begun a number of activities that we hope will enhance future ability to detect the presence of explosives vapors and residues. Here we present initial work on the development of a microfluidic system for a new point chemical sensor allowing the rapid, accurate, and specific detection of vapors emitted by explosive materials. We have already extensively tested a micromachined platform with external optical excitation and detection. Our new chemical approach is to create a receptor with high affinity and selectivity to nitro-explosives. A series of chemiluminescent molecular signaling systems are proposed that are specifically directed toward detection of TNT, PETN, RDX, HMX, and CL-20. These will be used in a new micromachined platform that integrates photodetectors directly into a micromachined micro-fluidic bead platform for detection of the chemiluminescent signals. By integrating photodetectors into the sidewalls of our chemical sensor array, in immediate proximity to the sensing microbeads, we can eliminate all external optics currently required for optical signal collection. This should allow a more compact and robust system to be constructed by integrating photodetection and fluidics into a single chip-based platform. Additionally, a concept of accessing a photodiode using inductive coupling, i.e. non-contact wireless reading, is introduced and demonstrated.

Park, Yoon; Neikirk, Dean P.; Anslyn, Eric V.

2006-09-01

302

Chemiluminescence behaviour of CdTe-potassium permanganate enhanced by sodium hexametaphosphate and sensitized sensing of L-ascorbic acid.  

PubMed

A highly sensitive flow-injection chemiluminescence (FIA-CL) method based on the CdTe nanocrystals and potassium permanganate chemiluminescence system was developed for the determination of L-ascorbic acid. It was found that sodium hexametaphosphate (SP), as an enhancer, could increase the chemiluminescence (CL) emission from the redox reaction of CdTe quantum dots with potassium permanganate in near-neutral pH conditions. L-ascorbic acid is suggested as a sensitive enhancer for use in the above energy-transfer excitation process. Under optimal conditions, the calibration graph of emission intensity against logarithmic l-ascorbic acid concentration was linear in the range 1.0?×?10(-9)-5.0?×?10(-6) ?mol/L, with a correlation coefficient of 0.9969 and relative standard deviation (RSD) of 2.3% (n?=?7) at 5.0?×?10(-7) ?mol/L. The method was successfully used to determine L-ascorbic acid in vitamin C tablets. The possible mechanism of the chemiluminescence in the system is also discussed. PMID:22223590

Chen, Hongqi; Ling, Bo; Yuan, Fei; Zhou, Cailing; Chen, Jingguo; Wang, Lun

2012-01-01

303

On-line chemiluminescence detector for hydrogen sulfide and methyl mercaptan  

SciTech Connect

The need for continuous monitoring of species like H/sub 2/S and CH/sub 3/SH in the atmosphere is obvious because of their toxic nature and is particularly important in synfuel production and geothermal energy utilization. Chemiluminescence under the right conditions is an extremely sensitive method, with the possibility of obtaining one photon for each molecule of interest. It is apparent that a selective detector should be based on emission from a species related chemically to the analyte rather than the reagent. ClO/sub 2/ is an interesting reagent in that it reacts, vide infra, with H/sub 2/S and CH/sub 3/SH to produce sulfur atoms, which recombine to form electronically excited S/sub 2/ and in turn result in fluorescence. To minimize the effects of quenching, one must keep the reaction zone at reduced pressures. To maximize sensitivity despite the high-order reaction, one must keep the volume of the reaction zone small, and the reagent gas (ClO/sub 2/) must be in excess to produce complete reaction in a pseudo-first-order regime. A successful on-line chemiluminescence detector is therefore dependent on meeting these various design parameters and is reported here.

Spurlin, S.R.; Yeung, E.S.

1982-02-01

304

Chemiluminescence Study on Thermal Degradation of Aircraft Tire Elastomers.  

National Technical Information Service (NTIS)

Since the autoxidative process accounts in part for the degradation of rubber, including aircraft tires, it was felt that a study of the chemiluminescence from unsaturated elastomers could contribute significantly to an understanding of the degradation me...

G. D. Mendenhall R. A. Nathan T. B. Stanford

1976-01-01

305

Europium-sensitized Chemiluminescence of System Tetracycline–H 2 O 2 –Fe(II)\\/(III) and Its Application to the Determination of Tetracycline  

Microsoft Academic Search

Chemiluminescence (CL) of the reaction system tetracycline–H2O2–Fe(II)\\/(III)–Eu(III) was used for the determination of tetracycline hydrochloride in water, pharmaceutical preparations,\\u000a and honey. The CL spectrum registered for this system shows emission bands typical of Eu(III) ions, with a maximum at ????600 nm,\\u000a corresponding to the electronic transitions of 5D0 ? 7F1 and 5D0 ? 7F2. A strong chemiluminescence intensity characteristic of europium(III)

M. Kaczmarek; A. Idzikowska; S. Lis

2008-01-01

306

Flow–injection chemiluminescence determination of ascorbic acid by use of the cerium(IV)–Rhodamine B system  

Microsoft Academic Search

A highly sensitive flow–injection (FI) method with chemiluminescence (CL) detection is used for the determination of l-ascorbic acid. The method is based on the CL reaction of Rhodamine B with cerium(IV) in sulfuric acid media. l-Ascorbic acid is suggested to be a catalyst utilized in the energy-transferred excitation process. The proposed procedure allows quantitation of l-ascorbic acid in the range

Yongjun Ma; Min Zhou; Xiaoyong Jin; Baozhu Zhang; Hui Chen; Naiyun Guo

2002-01-01

307

Flow injection chemiluminescence determination of naphazoline hydrochloride in pharmaceuticals.  

PubMed

A simple and sensitive flow injection chemiluminescence (FI-CL) method was developed for the determination of naphazoline hydrochloride (NPZ). The method is based on the enhancing effect of NPZ on the weak CL signal from the reaction of KIO4 with H2 O2 . Experimental parameters that affected the CL signal, including the pH of the KIO4 solution, concentrations of KIO4 , H2 O2 and disodium-EDTA and flow rate were optimized. Under the optimum conditions, the increment of CL intensity was linearly proportional to the concentration of NPZ in the range 5.0 × 10(-6) to 70 × 10(-6) mol/L. The detection limit was 1.0 × 10(-6) mol/L and the relative standard deviation for 50 × 10(-6) mol/L NPZ solution was 2.8% (n = 11). In addition, a high throughput of 120 samples/h was achieved. The utility of this method was demonstrated by determining NPZ in pharmaceuticals. PMID:23463582

Iranifam, Mortaza; Sorouraddin, Mohammad H

2014-02-01

308

Scanning laser vibrometry and luminol photomicrography to map cavitational activity around ultrasonic scalers  

NASA Astrophysics Data System (ADS)

Ultrasonic dental scalers are clinically used to remove deposits from tooth surfaces. A metal probe, oscillating at ultrasonic frequencies, is used to chip away deposits from the teeth. To reduce frictional heating, water flows over the operated probe in which a bi-product, cavitation, may be generated. The aim of this study is characterise probe oscillations using scanning laser vibrometry and to relate the recorded data to the occurrence of cavitation that is mapped in the course of this research. Scanning laser vibrometry (Polytec models 300-F/S and 400-3D) was used to measure the movement of various designs of operating probes and to locate vibration nodes / anti-nodes at different generator power settings and contact loads (100g and 200g). Cavitation mapping was performed by photographing the emission from a luminol solution with a digital camera (Artemis ICX285). The scaler design influences the number and location of vibration node / anti-node points. For all ultrasonic probes, the highest displacement amplitude values were recorded at the tip. The highest amounts of cavitation around the probes were recorded at the second anti-node measured from the tip. Broad, beaver-tale shaped probes produced more cavitation than slim shaped ones. The design also influences the amount of inertial cavitation around the operated instrument. The clinical relevance is that broad, beaver-tale shaped probes are unlikely to reach subgingival areas of the tooth. Further research is required to design probes that will be clinically superior to cleaning this area of the tooth.

Felver, Bernhard; King, David C.; Lea, Simon C.; Price, Gareth J.; Walmsley, A. Damien

2008-06-01

309

Aqueous nitrite ion determination by selective reduction and gas phase nitric oxide chemiluminescence  

NASA Technical Reports Server (NTRS)

An improved method of flow injection analysis for aqueous nitrite ion exploits the sensitivity and selectivity of the nitric oxide (NO) chemilluminescence detector. Trace analysis of nitrite ion in a small sample (5-160 microL) is accomplished by conversion of nitrite ion to NO by aqueous iodide in acid. The resulting NO is transported to the gas phase through a semipermeable membrane and subsequently detected by monitoring the photoemission of the reaction between NO and ozone (O3). Chemiluminescence detection is selective for measurement of NO, and, since the detection occurs in the gas-phase, neither sample coloration nor turbidity interfere. The detection limit for a 100-microL sample is 0.04 ppb of nitrite ion. The precision at the 10 ppb level is 2% relative standard deviation, and 60-180 samples can be analyzed per hour. Samples of human saliva and food extracts were analyzed; the results from a standard colorimetric measurement are compared with those from the new chemiluminescence method in order to further validate the latter method. A high degree of selectivity is obtained due to the three discriminating steps in the process: (1) the nitrite ion to NO conversion conditions are virtually specific for nitrite ion, (2) only volatile products of the conversion will be swept to the gas phase (avoiding turbidity or color in spectrophotometric methods), and (3) the NO chemiluminescence detector selectively detects the emission from the NO + O3 reaction. The method is free of interferences, offers detection limits of low parts per billion of nitrite ion, and allows the analysis of up to 180 microL-sized samples per hour, with little sample preparation and no chromatographic separation. Much smaller samples can be analyzed by this method than in previously reported batch analysis methods, which typically require 5 mL or more of sample and often need chromatographic separations as well.

Dunham, A. J.; Barkley, R. M.; Sievers, R. E.; Clarkson, T. W. (Principal Investigator)

1995-01-01

310

Can breath isoprene be measured by ozone chemiluminescence?  

PubMed

Isoprene, involved in the biosynthetic pathway to cholesterol, is the prevalent hydrocarbon in breath. Breath isoprene measurement is of great interest as a measure of basal cholesterol production rate. We investigated the merits and pitfalls of isoprene measurement via its chemiluminescence (CL) reaction with ozone. For many subjects, apparent concentrations measured are higher than those obtained by a gas chromatography (GC) reference method that can be traced to ozone-induced CL with simultaneously present lower olefins and sulfur compounds. A warm column preconcentration method eliminates the lower olefins and greatly improves sensitivity while a silver-form, ion-exchange resin can remove the sulfur gases. The breath sample is captured on a miniature synthetic carbon sorbent column maintained at 55 degrees C, under which conditions ethylene, propylene, and water vapor are not significantly captured while the preconcentration process greatly improves the limit of detection for isoprene to 0.6 ppbv (S/N=3). The captured isoprene is released by heating the column to 150 degrees C. Breath samples from different subjects were collected both before and after meals and analyzed in a double-blind fashion in two laboratories, with the second laboratory performing quantitation by cryofocusing GC-flame ionization detection with parallel measurement by mass spectrometry to provide identity confirmation. For all individuals studied, the CL and the GC results agreed when both warm column preconcentration and passage through Ag+-form cation-exchange resin, which removes divalent sulfur gases, were implemented prior to CL measurement. The intensity of CL from the reaction with ozone can be much higher for some sulfur gases than for isoprene. Even though present at lower concentrations than isoprene, unless removed prior to CL measurement, for some individuals sulfur gases can cause unacceptably large (up to 500%) errors, making the sulfur gas removal step critical. PMID:17326613

Ohira, Shin-Ichi; Li, Jianzhong; Lonneman, William A; Dasgupta, Purnendu K; Toda, Kei

2007-04-01

311

Chemiluminescent responses of alveolar macrophages from normal and Mycobacterium bovis BCG-vaccinated rabbits as a function of age.  

PubMed Central

Luminol-dependent chemiluminescence (CL) responses of alveolar macrophages (AM) from normal and Mycobacterium bovis BCG-vaccinated infant and adult rabbits were compared. AM from 1-, 7-, and 14-day-old normal rabbits exhibited much lower peak CL responses than did AM from 28- and 42-day-old normal animals as well as rabbits 2 to 3 or 5 to 6 months and 1 to 2 years of age. The most striking differences among AM from infant and adult rabbits were noted when AM were obtained from 28-day-old and 5- to 6-month old rabbits 21 days after the rabbits were immunized with 200 micrograms of BCG intravenously. In this case, AM from 5- to 6-month-old animals gave peak counts per minute of 400,000 to 500,000 whereas AM from 28-day-old rabbits vaccinated with BCG (harvested at 49 days of age) gave peak counts per minute of only 40,715 +/- 2,688. These data reveal that AM from neonatal animals are grossly deficient as responders to phorbol myristate acetate-induced CL. This deficiency, which improved with age, is still apparent in AM from 28-day-old animals. The data also reveal that BCG vaccination of 28-day-old animals yields AM that are poor responders to phorbol myristate acetate compared with AM from BCG-vaccinated animals 2 to 3 and 5 to 6 months of age. AM from animals vaccinated with BCG at 28 days of age contained fewer and smaller electron-dense lysosomelike structures than did AM from adult rabbits similarly vaccinated. These findings provide an explanation for the difficulties infants have in developing effective cell-mediated immune responses against intracellular parasites. Images

Chida, K; Myrvik, Q N; Leake, E S; Gordon, M R; Wood, P H; Ricardo, M J

1987-01-01

312

Mechanism of neutrophil chemiluminescence induced by wheat germ agglutinin: partial characterization of the antigens recognized by wheat germ agglutinin  

SciTech Connect

Wheat germ agglutinin (WGA) stimulated neutrophils to produce significant levels of luminol-dependent chemiluminescence (CL). Since WGA is known to bind N-acetylglucosamine (GlcNAc) oligomers and N-acetylneuraminic acid (NANA), we attempted to determine which binding property of WGA is essential for induction of CL. The succinylated form of WGA (SuWGA), which is no longer able to bind NANA, was still able to induce CL. N-Acetylglucosamine at a concentration of 20 mmol/L almost completely inhibited WGA-induced CL production by neutrophils, whereas bovine submaxillary gland mucin, a potent blocker of NANA binding of WGA, failed to inhibit CL production. Lectins with the GlcNAc-binding property were examined for their ability to induce CL. Those that have higher valences and have a tendency to bind GlcNAc oligomers in the internal portion of glycoconjugates were able to induce CL, whereas those that have low valences and bind terminal GlcNAc of glycoconjugates failed to induce CL even at high concentrations. Attempts were made to characterize the neutrophil membrane proteins recognized by WGA. Glycoproteins with a molecular weight of 25,000 daltons were identified by a 50 mmol/L GlcNAc elution of WGA gels loaded with /sup 125/I-labeled neutrophil membrane proteins. Elution with 500 mumol/L GlcNAc trimer produced several glycoproteins of different molecular weights in addition to the glycoproteins of 25,000 daltons. /sup 125/I-labeled WGA and SuWGA were used for autoradiographic analysis of cell extracts of the neutrophils separated on sodium dodecyl sulfate polyacrylamide gels. WGA recognized multiple glycoproteins of different molecular weights, whereas SuWGA bound only a few of them. Glycoproteins of 25,000 daltons, probably corresponding to those identified by 50 mmol/L GlcNAc elution, were also recognized.

Ozaki, Y.; Iwata, J.; Ohashi, T.

1984-11-01

313

Selective recognition of dysprosium(III) ions by enhanced chemiluminescence CdSe quantum dots.  

PubMed

The intensity of emitted light from CdSe quantum dots (QDs)-H2O2 is described as a novel chemiluminescence (CL) reaction for determination of dysprosium. This reaction is based on the catalytic effect of Dy(3+) ions, causing a significant increase in the light emission, as a result of the reaction of quantum dots (QDs) with hydrogen peroxide. In the optimum conditions, this method was satisfactorily described by linear calibration curve in the range of 8.3×10(-7)-5.0×10(-6)M, the detection limit of 6.0×10(-8)M, and the relative standard deviation for five determinations of 2.5×10(-6)M Dy(3+) 3.2%. The main experimental advantage of the proposed method is its selective to Dy(3+) ions compared with common coexisting cations, therefore, it was successfully applied for the determination of dysprosium ions in water samples. PMID:24231747

Hosseini, Morteza; Ganjali, Mohammad R; Vaezi, Zahra; Faridbod, Farnoush; Arabsorkhi, Batool; Sheikhha, Mohammad H

2014-03-01

314

Effect of Endotoxemia and Gram-Negative Sepsis on Host Peripheral Granulocyte Endogenous Chemiluminescence.  

National Technical Information Service (NTIS)

To evaluate the diagnostic potential of the chemiluminescence (CL) phenomenon for bacterial infections, polymorphonuclear chemiluminescence (PMN CL) was measured following challenge of rats with a gram-negative endotoxin-bearing microorganism, Salmonella ...

J. P. McCarthy R. S. Bodroghy P. Z. Sobocinski

1981-01-01

315

Determination of beta-lactam antibiotics in milk using micro-flow chemiluminescence system with on-line solid phase extraction.  

PubMed

In this paper, a chemiluminescence (CL) micro-flow system combined with on-line solid phase extraction (SPE) is presented for determination of beta-lactam antibiotics (penicillin, cefradine, cefadroxil, cefalexin) in milk. It is based on the enhancement effect of beta-lactam antibiotics on the luminol-K3Fe(CN)6 CL system. The micro-flow system was fabricated from two polymethyl methacrylate (PMMA) plates (50 mm x 40 mm x 5 mm) with the microchannels of 200 microm wide and 150 microm deep. C18-modified silica gel was packed into the microchannel (length: 10 mm; width: 1 mm; depth: 500 microm) to serve as SPE device. Extraction and preconcentration of the analytes were carried out using on-line SPE micro-flow system and the selectivity of CL detection was improved. The detection limits were 0.5 microg mL(-1) of penicillin, 0.04 microg mL(-1) of cefradine, 0.08 microg mL(-1) of cefadroxil and 0.1 microg mL(-1) of cefalexin. The proposed method was also applied to analyze the beta-lactam antibiotics in milk. Experimental results were in good agreement with those obtained by high performance liquid chromatography (HPLC) method with UV detection. PMID:17512825

Liu, Wei; Zhang, Zhujun; Liu, Zuoqin

2007-06-01

316

Highly sensitive chemiluminescence immunoassay on chitosan membrane modified paper platform using TiO2 nanoparticles/multiwalled carbon nanotubes as label.  

PubMed

A highly sensitive chemiluminescence (CL) immunoassay was incorporated into a low-cost microfluidic paper-based analytical device (?-PAD) to fabricate a facile paper-based CL immunodevice (denoted as ?-PCLI). This ?-PCLI was constructed by covalently immobilizing capture antibody on a chitosan membrane modified ?-PADs, which was developed by simple wax printing methodology. TiO2 nanoparticles coated multiwalled carbon nanotubes (TiO2/MWCNTs) were synthesized as an amplification catalyst tag to label signal antibody (Ab2). After sandwich-type immunoreactions, the TiO2/MWCNTs were captured on the surface of ?-PADs to catalyze the luminol-p-iodophenol-H2O2 CL system, which produced an enhanced CL emission. Using prostate-specific antigen as a model analyte, the approach provided a good linear response range from 0.001 to 20 ng/mL with a low detection limit of 0.8 pg/mL under optimal conditions. This ?-PCLI showed good reproducibility, selectivity and stability. The assay results of prostate-specific antigen in clinical serum samples were in good agreement with that obtained by commercially used electrochemiluminescence methods at the Cancer Research Center of Shandong Tumor Hospital (Jinan, Shandong Province, China). This ?-PCLI could be very useful to realize highly sensitive, qualitative point-of-care testing in developing or developed countries. PMID:23355319

Li, Weiping; Ge, Shenguang; Wang, Shoumei; Yan, Mei; Ge, Lei; Yu, Jinghua

2013-01-01

317

Alteration of monocytic cell oxidative burst caused by methacrylic monomers present in dental materials: a chemiluminescence study.  

PubMed

Methacrylates are present in dental composite resins used in clinical practice. Methacrylates are photo-polymerized, but this reaction is never complete, so release of uncured monomers in the periapical tissues and in biological fluids may happen and, potentially, alter the repair of pulpal and of periapical lesions by interfering with local phagocytes. The aim of this study was the evaluation of the functional activity of the monocyte-macrophage system after incubation with methacrylic monomers. The oxidative burst of two cellular systems was analysed using the chemiluminescence technique. Data were collected and statistically analysed. Monomers were found to reduce the in vitro oxidative burst of phagocytes independently from their cytotoxicity. These findings demand further evaluation of the effects of oxidative burst alteration in monocyte-macrophage function and may prompt the inclusion of the described chemiluminescence test in biocompatibility preliminary studies of dental materials. PMID:16645960

Nocca, Giuseppina; De Sole, Pasquale; Gambarini, Gianluca; De Palma, Francesco; Parziale, Vito; Giardina, Bruno; Lupi, Alessandro

2006-01-01

318

Comparison of the MAST chemiluminescent assay system with RAST and skin tests in allergic children.  

PubMed

The MAST chemiluminescent assay (CLA) system uses enzyme-linked antihuman IgE and a chemiluminogenic substance to determine specific IgE antibodies. The MAST CLA system therefore not only makes unnecessary the precautions required for handling isotopic materials but has the advantage of long-term reagent stability. The MAST CLA system assay protocol consists of three steps: overnight incubation of serum, a 4-hour incubation with enzyme-labeled antibody, and a 30-minute chemiluminescent reaction, which produces a visible image (immunograph) on high-speed instant Polaroid film. We evaluated this MAST CLA system in 47 allergic children and compared the results with those for RAST and skin tests. Good correlations were found between MAST and RAST for house dust, Dermatophagoides farinae, cat, Japanese cedar pollen, egg white, and soybeans, the correlation coefficients being greater than 0.60. When the diagnostic results for MAST and RAST were compared with those of skin test reactions for house dust, Dermatophagoides farinae, cat, Japanese cedar pollen, egg white, and soybeans, MAST had a sensitivity of 85% and a specificity of 82% as compared with 88% and 83% for RAST. Further, an average of 2.1 different allergens were detected in one serum sample. These results suggest that the MAST CLA system can be used to detect specific IgE antibodies and that it provides accurate results for allergic children. PMID:7679259

Agata, H; Yomo, A; Hanashiro, Y; Muraki, T; Kondo, N; Orii, T

1993-02-01

319

Urea Monitor Based on Chemiluminescence and Electrolysis as a Marker for Dialysis Efficiency  

NASA Astrophysics Data System (ADS)

We have developed a practical urea monitor based on a chemiluminescent (CL) reaction of urea and hypobromous acid produced by electrolysis of sodium bromide (NaBr) for measuring urea concentration in spent dialysate at set intervals. A reagent containing 4×10-2 M hypobromous acid is produced by electrolysis of an electrolyte containing 5.9 M NaBr and 0.2 M sodium hydroxide (NaOH). Chemiluminescence is emitted by injection of spent hemodialysis fluid (0.11 ml) into the reagent, and the CL-intensity is measured by a photomultiplier tube using the photon counting technique. The CL-intensity is proportional to the 0.9th power of the urea concentration between 7×10-4 and 2×10-2 M. The urea monitor can determine the urea concentration in spent dialysate samples collected from the waste line of a dialyzer, and the time for the intermittent measurements including the cleaning cycle of the reaction chamber is 3 min. The urea concentrations measured by the monitor are in close agreement with those measured by the conventional enzyme colorimetric method using urease for the spent dialysate collected during a hemodialysis treatment, and the correlation coefficient is 0.93.

Ozaki, Masahiro; Okabayashi, Tohru; Ishimaru, Teppei; Hayashi, Kunihito; Hori, Jun'ya; Yamamoto, Isao; Nakagawa, Masuo

320

Peritoneal macrophage activation indicated by enhanced chemiluminescence.  

PubMed Central

A number of studies have demonstrated the ability of various bacterial preparations, protozoa, and chemicals to activate macrophages and concomitantly to enhance host resistance to both tumors and infections. Recently, viral infections have been shown to have a similar effect upon macrophage function. To better define the metabolic state of activated macrophages, we have evaluated the ability of peritoneal cells (PC) from vaccinia virus- or murine cytomegalovirus-infected or Corynebacterium parvum-treated mice to emit chemiluminescence (CL) during phagocytosis of zymosan particles or yeasts. PC from C. parvum-treated mice (1,400 microgram intraperitoneally) emitted enhanced CL over controls on days 3, 6, 14, and 21 after treatment, thereby establishing the emission of CL as a correlate of metabolic activation. Previous evidence for activation of PC from vaccinia virus-infected mice (10(8) plaque-forming units) was confirmed by demonstration of enhanced levels of CL on days 3, 6, and 13 after murine infection. Likewise, PC from mice infected with murine cytomegalovirus (10(5) plaque-forming units) 3, 6, or 13 days previously demonstrated augmented levels of CL over controls. Opsonized virus particles (vaccinia virus or murine cytomegalovirus) failed to induce the emission of CL with PC from mice infected with the isologous virus. Our data further demonstrate the immunomodulationinduced by virus infections and suggest that the detection of CL is an easily quantitated correlate of macrophage activation which may be helpful in defining metabolic alterations induced during activation.

Schleupner, C J; Glasgow, L A

1978-01-01

321

Experimental model for the study by chemiluminescence of the activation of isolated equine leucocytes  

Microsoft Academic Search

The activation of human polymorphonuclear leucocytes (the respiratory burst) can be studied by measuring their chemiluminescent response. This technique was adapted to equine leucocytes to investigate the effects of cell number, activator concentration, enhancers of chemiluminescence, pH, temperature and inhibitors. Leucocytes were isolated from citrated blood from healthy horses and chemiluminescence was measured with a Bio-Orbit luminometer sensitive to 900

D Serteyn

1996-01-01

322

Chemiluminescence in the course of methyl ethyl ketone oxidation by ozone in acidic aqueous solutions  

Microsoft Academic Search

Chemiluminescence in the visible spectral region is observed in the course methyl ethyl ketone oxidation by ozone in neutral and acidic aqueous solutions. The spectral composition of the chemiluminescence is studied. On going from neutral to acidic aqueous solutions of methyl ethyl ketone, the initial intensity of the signal increases and the kinetics of a decrease in the chemiluminescence intensity

Yu. S. Zimin; A. A. Gusmanov; S. L. Khursan

2004-01-01

323

Composite poly(dimethylsiloxane)\\/glass microfluidic system with an immobilized enzymatic particle-bed reactor and sequential sample injection for chemiluminescence determinations  

Microsoft Academic Search

A three-layer poly(dimethylsiloxane) (PDMS)\\/glass microfluidic system for performing on-chip solid-phase enzymatic reaction and chemiluminescence (CL) reaction was used for the determination of glucose as a model analyte. A novel method for the immobilization of controlled-pore-glass based reactive particles on PDMS microreactor beds was developed, producing an on-chip solid-phase reactor that featured large reactive surface and low flow impedance. Efficient mixing

Zhang-Run Xu; Zhao-Lun Fang

2004-01-01

324

Real-time instrument for ambient monitoring of hydrogen sulfide, dimethyl sulfide, and other reduced organosulfur species using ozone chemiluminescent detection  

SciTech Connect

The chemiluminescent reactions of H/sup 2/S and other gaseous sulfides with ozone at 298/sup 0/K have been investigated, with the aim of developing a simple, selective, real-time monitor for these naturally emitted compounds. A commercial chemiluminescent ozone monitor has been adapted, for detection of the ozone/sulfide chemiluminescence between 300 and 400 nm wavelenght. Excess ozone was supplied to the reaction chamber from an electrical discharge ozone source. Response to dimethyl sulfide was enhanced by the use of air in the ozone source rather than oxygen. This effect was found to be caused by the presence of oxides of nitrogen produced in the ozonizer; a similar enhancement was not observed for the other sulfide compounds studied. Observed chemiluminescence signal decreased in the order CH/sub 3/SH > CH/sub 3/SCH/sub 3/ > H/sub 2/S > thiophene, with detection limits of 0.1, 0.3, 4, and 12 ppb respectively, at an instrument time constant of 60 sec. Calibration plots were linear at low sulfide concentrations, and showed less-than-first order dependence on sulfide at higher concentrations. A very small signal from ethylene was the only interference found from several compounds tested; sample air humidity has a small but significant effect on the instrument response.

Kelly, T.J.; Phillips, M.F.; Tanner, R.L.; Gaffney, J.S.

1982-01-01

325

Anodic electrogenerated chemiluminescence of quantum dots: size and stabilizer matter  

NASA Astrophysics Data System (ADS)

The electrogenerated chemiluminescence (ECL) of semiconductor quantum dots (QDs) is generally believed to be independent of particle sizes or the capping agents used. Herein, we demonstrate that CdTe QDs with different sizes and stabilizers evidently exhibit different ECL behavior in aqueous solution. The ECL of CdTe QDs stabilized by 3-mercaptopropionic acid (MPA) displays two waves at potentials of about +1.17 V and +1.74 V vs. Ag/AgCl, respectively. ECL spectra confirm that the ECL of QDs is attributed to their band gap luminescence, in which the peak positions are changed with QD sizes. The ECL mechanism of CdTe QDs involves superoxide radical generation by reduction of dissolved oxygen at lower potential or water splitting at higher potential. Direct evidence for superoxide radicals in this medium was obtained via electron spin resonance (ESR) experiments. In comparison, the 2-mercaptoethylamine (MEA)-capped CdTe QDs did not exhibit any ECL in air-saturated pH 7.4 PBS. Both ESR and X-ray photon spectroscopy (XPS) experiments revealed that amine groups in MEA-capped QDs were responsible for the absence of ECL. The reaction of an amine group with a superoxide radical leads to the quenching of ECL. The ECL quenching of MPA-capped CdTe QDs was further used to detect melamine. Under the optimum conditions, the inhibited ECL was linear with the logarithm of concentration of melamine within the concentration range of 10-9 to 10-5 M and the detection limit was found to be 6.74 × 10-10 M, which was 100-100 000 times lower than that of the most previous methods.The electrogenerated chemiluminescence (ECL) of semiconductor quantum dots (QDs) is generally believed to be independent of particle sizes or the capping agents used. Herein, we demonstrate that CdTe QDs with different sizes and stabilizers evidently exhibit different ECL behavior in aqueous solution. The ECL of CdTe QDs stabilized by 3-mercaptopropionic acid (MPA) displays two waves at potentials of about +1.17 V and +1.74 V vs. Ag/AgCl, respectively. ECL spectra confirm that the ECL of QDs is attributed to their band gap luminescence, in which the peak positions are changed with QD sizes. The ECL mechanism of CdTe QDs involves superoxide radical generation by reduction of dissolved oxygen at lower potential or water splitting at higher potential. Direct evidence for superoxide radicals in this medium was obtained via electron spin resonance (ESR) experiments. In comparison, the 2-mercaptoethylamine (MEA)-capped CdTe QDs did not exhibit any ECL in air-saturated pH 7.4 PBS. Both ESR and X-ray photon spectroscopy (XPS) experiments revealed that amine groups in MEA-capped QDs were responsible for the absence of ECL. The reaction of an amine group with a superoxide radical leads to the quenching of ECL. The ECL quenching of MPA-capped CdTe QDs was further used to detect melamine. Under the optimum conditions, the inhibited ECL was linear with the logarithm of concentration of melamine within the concentration range of 10-9 to 10-5 M and the detection limit was found to be 6.74 × 10-10 M, which was 100-100 000 times lower than that of the most previous methods. Electronic supplementary information (ESI) available: Fig. S1-S5 are available. See DOI: 10.1039/c2nr31324c

Hu, Tao; Li, Tangsong; Yuan, Lang; Liu, Shaoqin; Wang, Zhenlong

2012-08-01

326

Roles of CdS quantum dots in 1,1'-oxalyldiimidazole chemiluminescence.  

PubMed

The roles of CdS nanocrystals (quantum dots) stabilized by oleic acid ligands in toluene were investigated in the 1,1'-oxalyldiimidazole chemiluminescence (ODI-CL) reaction generated under various environmental conditions. CdS excited by high-energy intermediates formed in ODI-CL reaction emitted dim CL. Also, CdS acted as a catalyst to enhance the yield of high-energy intermediates, capable of transferring energy to fluorescent molecules, in aqueous ODI-CL reactions, whereas it acted as a strong quencher, capable of inhibiting the CL emission of excited fluorescent molecules, in non-aqueous ODI-CL reactions. Based on the role of CdS in the aqueous ODI-CL reaction, the limit of detection (LOD = signal/noise = 3, 0.1 ?M) determined to quantify glucose using aqueous ODI-CL reaction in the presence of 2.75 ?M CdS was four times lower than that in the absence of CdS. The range of recovery determined in the aqueous ODI-CL reaction in the presence of CdS was 91.7-104%. We expect that the aqueous ODI-CL reaction in the presence of CdS can be applied as a highly sensitive sensor in various research fields such as bioanalytical chemistry, environmental engineering, homeland security, and toxicology. PMID:23034656

Cho, Heejin; Lee, Syungju; Lee, Ji Hoon

2012-11-21

327

Determination of protoberberine alkaloids in medicinal plants based on acidic potassium permanganate chemiluminescence system.  

PubMed

A simple method was established to determine protoberberine alkaloids in Cortex Phellodendri and Rhizoma Coptidis based on an acidic potassium permanganate chemiluminescence (CL) system. The optimum conditions for the CL reaction between protoberberine alkaloids and potassium permanganate were studied in detail. Under the optimum conditions, the linear response ranges for berberine, palmatine and jatrorrhizine were 0.038-7.27, 0.031-18.1 and 0.012-3.61??g/mL with detection limits of 0.005, 0.004 and 0.0007??g/mL, respectively. This method was successfully applied to determine the content of protoberberine alkaloids (calculated using berberine as an index) in Cortex Phellodendri and Rhizoma Coptidis. In addition, a possible mechanism of this CL reaction was proposed on the basis of the investigation of CL, UV and fluorescent spectra of protoberberine alkaloids in acidic solution containing potassium permanganate. PMID:19743526

Xu, Xueqin; Lin, Qiang; He, Xiaoyan; Fu, FengFu; Chen, Guonan

2010-01-01

328

Enhanced chemiluminescence CdSe quantum dots by histidine and tryptophan.  

PubMed

The enhancing effect of histidine and tryptophan on chemiluminescence (CL) of CdSe quantum dots (QDs)-H2O2 system was studied. This reaction is based on the catalytic effect of amino acids, causing a significant increase in the light emission, as a result of the reaction of quantum dots (QDs) with hydrogen peroxide. In the optimum conditions, this method was satisfactorily described by linear calibration curve in the range of 0.66-35.5?M and 0.83-35.1?M for histidine and tryptophan, respectively. The effect of various parameters such as concentration of CdSe QDs, concentration of H2O2 and concentration of imidazole on the intensity of CL system were studied. The main experimental advantage of the proposed method is it's selective to two amino acids compared with other amino acids. PMID:24892543

Hosseini, Morteza; Ganjali, Mohammad Reza; Jarrahi, Afsaneh; Vaezi, Zahra; Mizani, Farhang; Faridbod, Farnoush

2014-11-11

329

Permanganate-bromide-silver nanoparticles as a new chemiluminescence system and its application to captopril determination.  

PubMed

A novel chemiluminescence (CL) system based on the oxidation of bromide by permanganate in sulfuric acid medium is introduced. The enhancing effect of silver nanoparticles (NPs), synthesized by chemical reduction method, on this reaction was studied. It was demonstrated that spherical silver nanoparticles with average size of 18 nm had a most remarkable catalytic effect on this reaction. CL emission wavelengths and UV-vis spectra were used to characterize the system and propose a possible mechanism. Furthermore, it was found that captopril inhibits the action of NPs and decreases the intensity of CL. Based on this phenomenon, a new CL method was developed for the determination of captopril in the 3.0 × 10(-10) to 1.0 × 10(-7) mol L(-1) concentration range with a detection limit (3s) of 0.12 nmol L(-1). The method was successfully applied to the determination of captopril in pharmaceutical formulations, human urine and serum samples. PMID:24054638

Amjadi, Mohammad; Manzoori, Jamshid L; Hassanzadeh, Javad; Sorouraddin, Mohammad H

2013-10-15

330

Chemiluminescence detection of cannabinoids and related compounds with acidic potassium permanganate.  

PubMed

This is the first report of chemiluminescence from the reaction of cannabinoids with acidic potassium permanganate, which we have applied to the high performance liquid chromatography (HPLC) determination of cannabidiol (CBD) in industrial-grade hemp. The intensities of the light-producing reactions with two commercially available cannabinoid standards were compared to that of seven model phenolic analytes. Resorcinol, representing the parent phenolic moiety of the cannabinoid class, was shown to react with the permanganate reagents in a manner more similar to phenol than to its hydroxyphenol positional isomers, pyrocatechol and hydroquinone. Alkyl substituents on the phenolic ring, however, have a considerable impact on emission intensity that is dependent upon the position of the groups and the composition of the permanganate reagent. This analytical approach has potential for the determination of other cannabinoids including ?(9) -tetrahydrocannabinol in drug-grade cannabis. PMID:22461321

Holland, Brendan J; Francis, Paul S; Li, Bingshan; Tsuzuki, Takuya; Adcock, Jacqui L; Barnett, Neil W; Conlan, Xavier A

2012-01-01

331

Enhanced chemiluminescence CdSe quantum dots by histidine and tryptophan  

NASA Astrophysics Data System (ADS)

The enhancing effect of histidine and tryptophan on chemiluminescence (CL) of CdSe quantum dots (QDs)-H2O2 system was studied. This reaction is based on the catalytic effect of amino acids, causing a significant increase in the light emission, as a result of the reaction of quantum dots (QDs) with hydrogen peroxide. In the optimum conditions, this method was satisfactorily described by linear calibration curve in the range of 0.66-35.5 ?M and 0.83-35.1 ?M for histidine and tryptophan, respectively. The effect of various parameters such as concentration of CdSe QDs, concentration of H2O2 and concentration of imidazole on the intensity of CL system were studied. The main experimental advantage of the proposed method is it’s selective to two amino acids compared with other amino acids.

Hosseini, Morteza; Ganjali, Mohammad Reza; Jarrahi, Afsaneh; Vaezi, Zahra; Mizani, Farhang; Faridbod, Farnoush

2014-11-01

332

Determination of oxides of nitrogen (NO/sub x/) in cigarette smoke by chemiluminescent analysis  

SciTech Connect

The successful application of a commercial chemiluminescent No/sub x/ analyzer to the determination of oxides of nitrogen in cigarette smoke is reported. Individual puffs of the smoke vapor phase are rapidly diluted in an air stream before introduction into the analyzer. This acts to both reduce quenching of the chemiluminescent response by CO/sub 2/ and to prevent side reactions of the NO/sub x/ with vapor phase organic constituents. Sweeping the dilute smoke through a reduced silver-ion exchange resin bed removed a substantial positive interference from hydrogen cyanide. A range of deliveries of 3 to 47 ..mu..mol of NO/sub x/ per cigarette was observed for nine types of experimental cigarettes. Statistically significant differences between NO/sub x/ and NO levels (NO/sub x/ - NO = NO/sub 2/) in smoke were observed in only one type of cigarette, presumably due to large cigarette-to-cigarette variability in constituent deliveries. 2 figures, 3 tables.

Jenkins, R.A.; Gill, B.E.

1980-05-01

333

The use of electrodialysis to prepare aqueous bread extracts for bromate determination by chemiluminescence.  

PubMed

A cleanup procedure based on electrodialysis is described for the preparation of aqueous bread extracts for bromate determination by chemiluminescence. The technique utilizes electrophoresis with 3 chambers separated by semipermeable membranes. The relative merits of reverse osmosis (RO), ultrafiltration, and nanofiltration membranes with various molecular weight cutoffs were evaluated. The best results were obtained with an RO membrane manufactured from thin-film (composite) polysulfone as support for polyamide. A 0.14 M sodium sulfate solution in the center or collection chamber provides optimum conductivity. Aqueous hydroxylamine sulfate (30 mM) was selected for the anode compartment as a reductant for the anode oxidation products. The constant current mode at 150 mA with a potential of ca 100 volts was used. After electrophoretic separation, the bromate concentration in the collection chamber was typically 2 to 3 times greater than the concentration in the bread extract. The chemiluminescent reaction of bromate with sulfite with hydrocortisone as the enhancer was selected for detection of bromate. The emission, with a wavelength maximum at 575 nm, was found to "glow" rather than "flash" after the reagents were mixed; therefore, it was possible to optimize the light collection period. The method was validated with a variety of commercial bread products. White bread, hot dog buns, hamburger rolls, and a multigrain bread from 7 different manufacturers were studied. PMID:16001854

Himata, Katsuichi; Warner, Charles; Currie, Douglas; Graves, Qian; Diachenko, Gregory

2005-01-01

334

Simultaneous determination of total IgE and allergen-specific IgE in serum by the MAST chemiluminescent assay system.  

PubMed

We have developed a chemiluminescent immunoenzymometric system. The first commercial application of this chemiluminescent assay (CLA) is the measurement of total IgE and allergen-specific IgE in human serum. The CLA system is a second-generation adaptation of the MAST RIA allergy profiling system. The MAST CLA system assay protocol consists of three steps: overnight incubation of serum, a 4-h incubation with enzyme-labeled antibody, and a 30-min chemiluminescent reaction, which produces a visible image (immunograph) on high-speed Polaroid instant film. The densities of the bands produced on the film are quantified with an inexpensive microprocessor-controlled infrared transmittance densitometer. The novel luminogenic substrates used yield a constant light output for over 2 h with an intensity at least 10-fold greater than that of commercial chemiluminescent reagents. The MAST CLA system exhibits sensitivity, specificity, and precision equal to that of the MAST RIA system (r = 0.96 for 40 serum samples analyzed with 25 allergens). As many as 35 different allergens per sample can be quantified in a single assay. The MAST CLA system requires no standard curve or volume-dependent pipetting steps, incorporates both positive and negative controls for each sample, and quantifies allergen-specific IgE at picomolar concentrations. PMID:4028399

Brown, C R; Higgins, K W; Frazer, K; Schoelz, L K; Dyminski, J W; Marinkovich, V A; Miller, S P; Burd, J F

1985-09-01

335

Analysis of the addition products of alpha-tocopherol with phosphatidylcholine-peroxyl radicals by high-performance liquid chromatography with chemiluminescent detection.  

PubMed

A chemiluminescence-based high-performance liquid chromatographic method was developed for the analysis of the addition products of alpha-tocopherol with phosphatidylcholine-peroxyl radicals (TOO-PC). The TOO-PC eluted from a reversed-phase column was reacted with a chemiluminescent reagent consisting of a Cypridina luciferin analog and a lipid-soluble iron chelate in acidic methanol at 50 degrees C, and the generated chemiluminescence was monitored. The detection limit for TOO-PC by this method was about 1 pmol. This method was applied to the detection of TOO-PC in the peroxidized membranes prepared from rabbit erythrocyte ghosts. When the erythrocyte ghosts were peroxidized by the addition of a water-soluble free radical initiator, a peak corresponding to TOO-PC was detected on the chromatogram with chemiluminescent detection. The amount of TOO-PC in the erythrocyte membranes increased with the depletion of endogenous alpha-tocopherol. The results indicate that this method proved useful for the detection of the TOO-PC formed by the peroxyl-radical scavenging reactions of alpha-tocopherol in biological systems. PMID:11202003

Yamauchi, R; Hara, Y; Murase, H; Kato, K

2000-12-01

336

Determination of pentachlorophenol and 1-naphthol by peroxyoxalate chemiluminescence  

SciTech Connect

Peroxyoxalate chemiluminescence is used for the determination of pentachlorophenol and 1-naphthol in soil. 1-Naphthol is brominated to make it amenable to the procedure. Calibration curves are linear over concentration ranges spanning four orders of magnitude and limits of detection are at the micromolar level. The processes can be carried out in the soil without prior extraction of the analytes.

Chimeno, J.; von Wandruszka, R. (Univ. of Idaho, Moscow (United States))

1989-01-01

337

Chemiluminescent detection of carbohydrates in the tumoral breast diseases.  

PubMed

Nowadays, there is an increase of investigations into the fibroadenoma, mainly because some studies have shown that the occurrence of fibroadenoma is linked to an increased risk of developing breast carcinoma. Currently, the chemiluminescence biomarkers are applied for validation methods and screening. Here, a lectin chemiluminescence is proposed as new histochemistry method to identify carbohydrates in mammary tumoral tissues. The lectins concanavalin A (Con A) and peanut agglutinin (PNA) conjugated to acridinium ester were used to characterize the glycocode of breast tissues: normal, fibroadenoma, and invasive duct carcinoma (IDC). The lectin chemiluminescence expressed in relative light units (RLU) was higher in fibroadenoma and IDC than in normal tissue for both lectins tested. The relationship RLU emission versus tissue area described a linear and hyperbolic curve for IDC and fibroadenoma, respectively, using Con A whereas hyperbolic curves for both transformed tissues using PNA. RLU was abolished by inhibiting the interaction between tissues and lectins using their specific carbohydrates: methyl-?-D: -mannoside (Con A) and galactose (PNA). The intrinsic fluorescence emission did not change with combination of the lectins (Con A/PNA) to the acridinium ester for hydrophobic residues. These results represent the lectin chemiluminescence as an alternative of histochemistry method for tumoral diagnosis in the breast. PMID:22068691

Brustein, Vanessa Passos; Cavalcanti, Carmelita Lima Bezerra; de Melo-Junior, Mario Ribeiro; Correia, Maria Tereza Santos; Beltrão, Eduardo Isidoro Carneiro; Carvalho, Luiz Bezerra

2012-01-01

338

Brain chemiluminescence and oxidative stress in hyperthyroid rats.  

PubMed Central

Newborn Wistar rats were made hyperthyroid by injection of tri-iodothyronine and assayed for survival, brain oxygen uptake, brain chemiluminescence and activity of antioxidant enzymes. Brain chemiluminescence was measured (1) by removing the parietal bones or (2) through the translucid parietal bones. Control animals showed a brain chemiluminescence of 130 +/- 12 c.p.s./cm2 and 99 +/- 10 c.p.s./cm2 for procedures (1) and (2) respectively. Hyperthyroid rats showed increases in the spontaneous brain photoemission of 46 and 70% compared with controls, measured by procedures 1 and 2 respectively. The hyperthyroid state did not modify the oxygen-dependent chemiluminescence of brain homogenates. The hyperthyroid animals showed a 30% increase in the oxygen uptake of brain slices and a dramatic shortening of life-span to about 16 weeks. Superoxide dismutase (the Cu-Zn enzyme), catalase and Se-dependent glutathione peroxidase activities of brain homogenates were increased by 18, 36 and 30% respectively in the hyperthyroid animals. Isolated brain mitochondria produced 0.18-0.20 nmol of H2O2/min per mg of protein in state 4 in the presence of succinate as substrate. No difference was observed between control and hyperthyroid animals. It is concluded that hyperthyroidism leads to hypermetabolism and oxidative stress in the brain. The increased levels of oxygen and peroxyl radicals may contribute to premature ageing in these animals.

Adamo, A M; Llesuy, S F; Pasquini, J M; Boveris, A

1989-01-01

339

Chemiluminescent emission spectra of tin mono- and di-halides  

Microsoft Academic Search

Chemiluminescent emission from flames of tin halides burning at low pressure in potassium vapor is found to include vibronic bands of mono- and di-halides. The emitters are, quite certainly, electrically neutral species. Bands from SnCl2 are analyzed with the aid of a Deslandres scheme and some vibrational constants are assigned to the upper and lower states.

David Naegeli; Howard B. Palmer

1966-01-01

340

Proximity hybridization-triggered signal switch for homogeneous chemiluminescent bioanalysis.  

PubMed

A proximity hybridization-triggered signal switch was presented for convenient homogeneous chemiluminescent detection of a wide range of affinity target biomolecules, such as oligonucleotides, protein biomarkers, and aptamer-recognized targets. The presence of the target promoted the formation of a proximate complex via the proximity hybridization of two help DNA strands or the DNA strands labeled to affinity ligands, which subsequently unfolded the self-reporting molecular beacon to switch on the chemiluminescence signal. The response could be further amplified with an in situ enzymatic recycling strategy for highly sensitive chemiluminescence detection. By using an antibody as the affinity ligand, this simple protocol could sensitively detect protein biomarker in a concentration range of 6 orders of magnitude with a detection limit down to 80 fM. With the use of an aptamer as the affinity ligand, a method for homogeneous chemiluminescent detection of thrombin was proposed. The one-step and wash-free assay showed good selectivity and required only 1 ?L of sample. Its high sensitivity, acceptable accuracy, and satisfactory versatility of analytes led to various applications in bioanalysis. PMID:24832725

Zong, Chen; Wu, Jie; Liu, Mengmeng; Yang, Linlin; Liu, Lin; Yan, Feng; Ju, Huangxian

2014-06-01

341

Signal-on dual-potential electrochemiluminescence based on luminol-gold bifunctional nanoparticles for telomerase detection.  

PubMed

Here, we report a novel type of signal-on dual-potential electrochemiluminescence (ECL) approach for telomerase detection based on bifunctionalized luminol-gold nanoparticles (L-Au NPs). In this approach, CdS nanocrystals (NCs) were first coated on glassy carbon electrode, and then thiol-modified telomerase primer was attached on CdS NCs via Cd-S bond. In the presence of telomerase and dNTPs, the primer could be extended. Telomerase primer would hybridize with its complementary DNA, and the extended part would hybridize with the capture DNA which was tagged with L-Au NPs. In the presence of coreactant H2O2, the L-Au NPs could not only enhance the ECL intensity of CdS NCs at -1.25 V (vs SCE) induced by the surface plasmon resonance (SPR) of Au NPs but also produce a new ECL signal at +0.45 V (vs SCE) that resulted from luminol in L-Au NPs. Both signals at two potentials increased with the increase of telomerase concentration. This method could be used to detect the telomerase from 100 to 9000 HL-60 cells and investigate the apoptosis of tumor cells. The ratio of the two signal increments (?ECLLuminol/?ECLCdS NCs), which showed a high consistency value for different numbers of cells, could be used to verify the reliability of tests. This dual-potential ECL strategy showed great promise in avoiding false positive or negative results in bioanalysis. PMID:24646287

Zhang, Huai-Rong; Wu, Mei-Sheng; Xu, Jing-Juan; Chen, Hong-Yuan

2014-04-15

342

Novel Chemiluminescent Substrate and Probe Systems for the Identification of CFDeltaF508 Genotypes.  

PubMed

Background: Chemiluminescence detection systems are rapidly gaining popularity as safer alternatives to isotopic methods in molecular diagnostics with equal sensitivity and specificity. In addition, they offer versatility of detection because of the availability of different haptens for labeling the probes, the antihapten antibodies conjugated with either alkaline phosphatase (AP) or horseradish peroxidase (HRP), and their respective chemiluminescent substrates. A novel dual chemiluminescent substrate (AP and HRP based) and probe systems to distinguish genotypes of cystic fibrosis DeltaF(508) mutation are described. Methods and Results: Two methodologies have been formulated to identify positively the genotypes of the cystic fibrosis DeltaF(508) mutation. In method 1, a pair of oligonucleotides designed to anneal to the fanking regions of DeltaF(508) mutation are differentially labeled with the hapten biotin or fluorescein and ligated using the template DNA of wild-type (N/N), heterozygous (N/DeltaF(508)), and homozygous (DeltaF(508)/DeltaF(508)) genotypes. The ligated product containing both labels is detected by first binding with avidin-HRP and anti-fluorescein-AP followed by reaction with the dual substrate. As expected, the ligation products are detected only in n/DeltaF(508) and DeltaF(508)/DeltaF(508) genotypes but not in N/N, where the ligation is precluded by the presence of three intervening nucleotides. In method 2, the three genotypes are hybridized on a membrane simultaneously with uniquely labeled (biotin or digoxigenin) oligonucleotides each designed to bind either the normal or the mutant allele. On treatment with HRP- and AP-conjugated antibodies followed by reaction with the dual substrate, only the band from N/DeltaF(508) genotype emitted a strong signal because of the binding of both oligonucleotides. Conclusions: The ligation and hybridization methods in conjunction with the dual substrate can detect and differentiate the genotypes with the DeltaF(508) mutation. These formats may be valuable for distinguishing normal individuals from carriers in population screening and fetuses that are heterozygous, from those that are homozygous for cystic fibrosis DeltaF(508) in prenatal and neonatal diagnosis. PMID:10462598

Reddy; Akhavan-Tafti; DeSILVA; Handley; Farkas; Schap

1997-06-01

343

Determination of spectrum and different temperature of spontaneous chemiluminescence in rice seeds during early imbibition  

NASA Astrophysics Data System (ADS)

With high-sensitivity single-photon counter, spontaneous chemiluminescence (CL) spectrum and different temperature study on rice (Oryza sativa L.) seeds during early imbibition were carried out. The emission spectrum of whole rice seed, rice and coat had a greater proportion of red light during early imbibition. Comparing with spontaneous CL of barley (Hordeum vulgare L.) and soybean (Glycine max L. Merr) seeds, the spontaneous CL of rice seeds had a nonlinear, logarithmic-like increase of intensity in the T range 30-50°C, the Van't Hoff coefficient Q10=IT+10/IT is equal to 2, which led us to the conclusion that spontaneous CL of rice seed during early imbibition partly came from enzyme catalyzing chemistry reaction.

Chen, WenLi; Xing, Da; Van Wijk, Roeland

2005-02-01

344

Photographic detection of fluorescent-labelled oligodeoxynucleotide in the blotting format by peroxyoxalate chemiluminescence.  

PubMed

The preparation of a fluorescent labelled oligonucleotide and its photographic detection by peroxyoxalate chemiluminescence (PO-CL) are described. Fluorescent labelling of an oligonucleotide (15-mer) was performed with naphthalene-2,3-dicarboxaldehyde to give an N-substituted 1-cyanobenz[f]isoindole (CBI) derivative (CBI-15-mer). For the photographic detection of CBI-15-mer, the bis(2,6-difluorophenyl) oxalate (DFPO)-dimethyl phthalate (DMP) system was selected to obtain a long-lived CL emission. After optimizing the conditions for the CL reaction, the system was applied to the photographic detection, and as little as 250 fmol per spot of CBI-15-mer on a membrane were detected as a visible spot with an instant photographic film. PMID:9633013

Kuroda, N; Hosoki, S; Nakashima, K; Akiyama, S; Givens, R

1998-01-01

345

Investigation of the kinetics of OH? and CH? chemiluminescence in hydrocarbon oxidation behind reflected shock waves  

NASA Astrophysics Data System (ADS)

The temporal variation of chemiluminescence emission from OH?(A2 ? +) and CH?(A2 ?) in reacting Ar-diluted H2/O2/CH4, C2H2/O2 and C2H2/N2O mixtures was studied in a shock tube for a wide temperature range at atmospheric pressures and various equivalence ratios. Time-resolved emission measurements were used to evaluate the relative importance of different reaction pathways. The main formation channel for OH? in hydrocarbon combustion was studied with CH4 as benchmark fuel. Three reaction pathways leading to CH? were studied with C2H2 as fuel. Based on well-validated ground-state chemistry models from literature, sub-mechanisms for OH? and CH? were developed. For the main OH?-forming reaction CH+O2=OH?+CO, a rate coefficient of k 2=(8.0±2.6)×1010 cm3 mol-1 s-1 was determined. For CH? formation, best agreement was achieved when incorporating reactions C2+OH=CH?+CO ( k 5=2.0×1014 cm3 mol-1 s-1) and C2H+O=CH?+CO ( k 6=3.6×1012exp(-10.9 kJ mol-1/ RT) cm3 mol-1 s-1) and neglecting the C2H+O2=CH?+CO2 reaction.

Bozkurt, M.; Fikri, M.; Schulz, C.

2012-06-01

346

Tomographic reconstruction of 2D-OH?-chemiluminescence distributions in turbulent diffusion flames  

NASA Astrophysics Data System (ADS)

A recently developed fast tomographic reconstruction device (Anikin et al. in Appl. Phys. B 100:675, 2010) has been applied to detect 2-D chemiluminescence distributions of OH? in reaction zones of a near laminar and a turbulent diffusion flame. A series of single-shot experiments has been carried out in both flames offering cold gas flow velocities of 0.43 m/s and 4 m/s and flame diameters up to 60 mm, respectively. The emission of OH?-chemiluminescence originating from the reaction zones of the flame fronts was registered by ten Kepler-telescopes surrounding the object under investigation at different pre-defined angles. The signals emerging from each telescope are collected by a fiber cable consisting of 90 single fibers arranged side by side in a single row, respectively. The signals originating from the ten cables/10×90=900 fibers represent the corresponding Radon transforms. These signals are imaged by a relay-optics onto the photocathode of a single image intensified CCD-camera. The output data of the camera are used for the reconstructions of the 2D-distributions of OH?-emission using a numerical procedure solving the inverse problem of tomography (Anikin et al. in Appl. Phys. B 100:675, 2010, and references therein). From the experimental results it is shown that the reconstructions obtained at exposure times down to 200 ?s reproduce fine structures of the flames with a spatial resolution of ˜1 mm. Therefore, the method is a useful tool for the detailed investigation of turbulent combustion.

Anikin, N. B.; Suntz, R.; Bockhorn, H.

2012-06-01

347

Detection of lanthanides and actinides in solutions based on laser-induced luminescence and chemiluminescence  

NASA Astrophysics Data System (ADS)

This work is devoted to applications of the time-resolved laser- induced luminescence spectroscopy and time-resolved laser-induced chemiluminescence spectroscopy for detection of lanthanides and actinides. The limit of detection (LOD) of some lanthanides and actinides in solutions by the time-resolved laser luminescence spectroscopy is up to 10-13 mole/liter. Unfortunately, Pu, Np, and some U compounds do not produce luminescence in solutions, but when excited by laser radiation, they can induce chemiluminescence. The characteristics of chemiluminescence induced by excited lanthanide and actinide complexes in solutions are considered. A key problem of chemiluminescence application for lanthanide and actinide detection in solutions is the increase of the detection selectivity. This problem can be solved by using the multistep schemes of chemiluminescence excitation. It is shown that the time-resolved luminescence laser spectroscopy and chemiluminescence laser spectroscopy can be successfully used for detection of lanthanides and actinides in solutions.

Izosimov, I. N.; Firsin, N. G.; Gorshkov, N. G.; Nekhoroshkov, S. N.

2014-06-01

348

Ultrasensitive luminol electrochemiluminescence for protein detection based on in situ generated hydrogen peroxide as coreactant with glucose oxidase anchored AuNPs@MWCNTs labeling  

Microsoft Academic Search

In this study, an ultrasensitive luminol electrochemiluminescence (ECL) immunosensor was constructed using carboxyl group functionalized multi-walled carbon nanotubes (MWCNTs) as platform and glucose oxidase (GOD) supported on Au nanoparticles (AuNPs) decorated MWCNTs (AuNPs@MWCNTs–GOD) as labels. Firstly, using poly(ethylenimine) (PEI) as linkage reagents, AuNPs@MWCNTs were prepared and introduced for binding of the secondary antibody (Ab2) and glucose oxidase (GOD) with high

Yaling Cao; Ruo Yuan; Yaqin Chai; Li Mao; Huan Niu; Huijing Liu; Ying Zhuo

349

Gas-phase chemiluminescence and chemi-ionization  

SciTech Connect

The phenomena of chemiluminescence (or more broadly chemi-excitation) and chemi-ionization have major similarities from a fundamental kinetic and dynamic point of view. However, since the former has primarily been investigated using optical spectroscopic techniques and the latter largely by mass spectroscopic (and other gaseous electronic) methods, the two phenomena have apparently never, explicitly been discussed together in one volume. In addition to a number of review articles on each individual subject, several meetings and books have had chemiluminescence and bioluminescence as their theme and those have been dominated by condensed phase work. On the other hand, chemi-ionization is often discussed in the contest of gaseous electronics, plasma chemistry and combustion. It is the goal of this book to present a more unified understanding of the two phenomena.

Fontijn, A.

1985-01-01

350

Immobilized fluorophores in dynamic chemiluminescence detection of hydrogen perioxide  

SciTech Connect

It is shown that the peroxy oxalate chemiluminescence system for the detection of H/sub 2/O/sub 2/ can be greatly simplified by using immobilized fluorophores. Various immobilization procedures for 3-aminofluoranthene are discussed. The chemiluminescence properties of materials based on cellulose, silica gel, and glass beads are compared. Controlled pore glass (CPG-10) was found to be the most suitable carrier. The material, packed in a quartz cell, is applied in a flow injection system in combination with a bed reactor containing bis(2,4,6-triphenyl) oxalate (TCPO) in solid form. For the determination of hydrogen peroxide in rain water samples, detection limits of approx.1 x 10/sup -8/ M H/sub 2/O/sub 2/ (0.3 ppb) were obtained using a homemade luminescence detector. Linear calibration curves up to 10/sup -5/ M were observed. 19 references, 7 figures, 1 table.

Guebitz, G.; van Zoonen, P. Gooijer, C.; Velthorst, N.H.; Frei, R.W.

1985-09-01

351

Metal-enhanced chemiluminescence: Radiating plasmons generated from chemically induced electronic excited states  

PubMed Central

In this letter, we report the observation of metal-enhanced chemiluminescence. Silver Island films, in close proximity to chemiluminescence species, can significantly enhance luminescence intensities; a 20-fold increase in chemiluminescence intensity was observed as compared to an identical control sample containing no silver. This suggests the use of silver nanostructures in the chemiluminescence-based immunoassays used in the biosciences today, to improve signal and therefore analyte detectability. In addition, this finding suggests that surface plasmons can be directly excited by chemically induced electronically excited luminophores, a significant finding toward our understanding of fluorophore-metal interactions and the generation of surface plasmons.

Chowdhury, Mustafa H.; Aslan, Kadir; Malyn, Stuart N.; Lakowicz, Joseph R.; Geddes, Chris D.

2009-01-01

352

Applications of dioxetane chemiluminescent probes to molecular biology  

SciTech Connect

DNA probes and synthetic oligonucleotides in general present one of the key tools in modern molecular biology research and increasingly also in commercial applications. Along with the many applications that have been developed for and with DNA probes, faster and more sensitive detection methods are being developed. One of the most promising recent developments presents a method based on enzymatically triggered chemiluminescence. Details of this chemistry along with applications in molecular biology and immunology are discussed and compared to conventional methods.

Beck, S.; Koester, H. (MilliGen/Biosearch, Burlington, MA (USA))

1990-11-01

353

Urinary free deoxypyridinoline by chemiluminescence immunoassay: analytical and clinical evaluation  

Microsoft Academic Search

We evaluated an automated chemiluminescence immu- noassay (CLIA) developed for the measurement of uri- nary free deoxypyridinoline (DPD). The new DPD method by CLIA is based on the competition of DPD with particle-bound pyridinoline for a limited amount of monoclonal mouse anti-DPD antibody. Total impre- cision (CV) was 3.2-9.0% at 30 -270 nmol\\/L. Regression analysis of urinary DPD concentration (second

Thomas G. Rosano; Robert T. Peaston; Henry G. Bone; Henning W. Woitge; Roger M. Francis; Markus J. Seibel

354

Chemiluminescence detection of heroin in illicit drug samples.  

PubMed

Heroin (3,6-diacetylmorphine) and several important extraction and synthesis impurities (morphine, 6-monoacetylmorphine, codeine and 6-acetylcodeine) were determined in illicit drug samples, using high performance liquid chromatography with 'parallel segmented flow', which enabled the simultaneous use of three complementary modes of detection (UV-absorbance, tris(2,2'-bipyridine)ruthenium(III) chemiluminescence and permanganate chemiluminescence). This rapid and sensitive approach for the analysis of street heroin was used to explore the chemistry of a proposed heroin screening test that is based on the relative response with these two chemiluminescence reagents using flow injection analysis. Although heroin was the major constituent of the six drug samples (between 16% and 67% by mass), the synthetic by-product 6-acetylcodeine (2.5-8.3%) made a greater contribution to the total [Ru(bipy)3](3+) chemiluminescence response of the screening test. The signal with permanganate was primarily due to the presence of 6-monoacetylmorphine (0.9-29%), and was therefore indicative of the degree of sample degradation during clandestine manufacture or poor storage conditions prior to the drug seizure. In the second part of the screening test, the sample is treated with sodium hydroxide, which results in a large increase in the signal with permanganate, due to the rapid hydrolysis of heroin to 6-monoacetylmorphine. As the emission of these two reagents with morphinan-alkaloids and their derivatives largely depends on the substituent at the O(3) position, the slower hydrolysis of 6-monoacetylmorphine to morphine, and 6-acetylcodeine to codeine, did not have a major impact on the characteristic pattern of responses in the screening test. PMID:24148453

Terry, Jessica M; Smith, Zoe M; Learey, Jessica J; Shalliker, R Andrew; Barnett, Neil W; Francis, Paul S

2013-11-15

355

The effective peroxidase-like activity of chitosan-functionalized CoFe2O4 nanoparticles for chemiluminescence sensing of hydrogen peroxide and glucose.  

PubMed

Here, we report a highly simple and general protocol for functionalization of the CoFe(2)O(4) NPs with chitosan polymers in order to make CoFe(2)O(4) NPs disperse and stable in solution. The functionalized CoFe(2)O(4) NPs (denoted as CF-CoFe(2)O(4) NPs) were characterized by scanning electron microscope (SEM), thermogravimetric (TG), X-ray diffraction (XRD) and FT-IR spectra. It was found that the CoFe(2)O(4) NPs were successfully decorated and uniformly dispersed on the surface of chitosan without agglomeration. The CF-CoFe(2)O(4) NPs were found to increase greatly the radiation emitted during the CL oxidation of luminol by hydrogen peroxide. Results of ESR spin-trapping experiments demonstrated that the CF-CoFe(2)O(4) NPs showed catalytic ability to H(2)O(2) decomposition into ?OH radicals. On this basis, a highly sensitive and rapid chemiluminescent method was developed for hydrogen peroxide in water samples and glucose in blood samples. Under optimum conditions, the proposed method allowed the detection of H(2)O(2) in the range of 1.0 × 10(-9) to 4.0 × 10(-6) M and glucose in the range of 5.0 × 10(-8) to 1.0 × 10(-5) M with detectable H(2)O(2) as low as 500 pM and glucose as low as 10 nM, respectively. This proposed method has been successfully applied to detect H(2)O(2) in environmental water samples and glucose in serum samples with good accuracy and precision. PMID:22258160

Fan, Yingwei; Huang, Yuming

2012-03-01

356

In vivo imaging using polymeric nanoparticles stained with near-infrared chemiluminescent and fluorescent squaraine catenane endoperoxide†  

PubMed Central

Polystyrene nanoparticles stained with squaraine catenane endoperoxide exhibit remarkably high chemiluminescence and enable optical imaging of biodistribution in living mice. Whole-body chemiluminescence imaging was much more effective than fluorescence at identifying lung accumulation of the nanoparticles.

Lee, Jung-Jae; White, Alexander G.; Rice, Douglas R.; Smith, Bradley D.

2013-01-01

357

Quantitation method of N,N '-disalicylidene-1,2-propanediamine by comprehensive two-dimensional gas chromatography coupled to a nitrogen chemiluminescence detector.  

PubMed

Metal deactivator additives (MDAs) have been used for over 60 years to prevent metal catalyzed reactions in petroleum products; a commonly used metal deactivator is N,N'-disalicylidene-1,2-propanediamine. The quantitation of low MDA concentrations in fuels is challenging due to the complexity of the sample matrix. In this work, this difficulty was overcome using GC × GC hyphenated with a nitrogen chemiluminescence detector. The high resolution power of GC × GC avoided co-elution between the MDA and other sample matrix compounds; while the enhanced sensitivity of GC × GC and the use of a nitrogen chemiluminescence detector supplied a high sensitivity and specificity for nitrogen compounds. For the analysis, the MDA additive was derivatized with the silylation agent N,O-bis (trimethylsilyl)trifluoroacetamide at room temperature and its quantitation was based on an external calibration curve; good linear response was obtained in the 1.4-8.6 ppm range. PMID:23494992

Lissitsyna, Kristina; Huertas, Sonia; Quintero, Luis Carlos; Polo, Luis Maria

2013-06-01

358

Magnetic-particle-based, ultrasensitive chemiluminescence enzyme immunoassay for free prostate-specific antigen.  

PubMed

We report a magnetic-particle (MMP)-based chemiluminescence enzyme immunoassay (CLEIA) for free prostate-specific antigen (f-PSA) in human serum. In this method, the f-PSA is sandwiched between the anti-PSA antibody coated MMPs and alkaline phosphatase (ALP)-labeled anti-f-PSA antibody. The signal produced by the emitted photons from the chemiluminescent substrate (4-methoxy-4-(3-phosphatephenyl)-spiro-(1,2-dioxetane-3,2'-adamantane)) is directly proportional to the amount of f-PSA in a sample. The present MMP-based assay can detect f-PSA in the range of 0.1-30 ng mL(-1) with the detection limit of 0.1 ng mL(-1). The linear detection range could match the concentration range within the "diagnostic gray zone" of serum f-PSA levels (4-10 ng mL(-1)). The detection limit was sufficient for measuring clinically relevant f-PSA levels (>4 ng mL(-1)). Furthermore, the method was highly selective; it was unaffected by cross-reaction with human glandular kallikrein-2, a kallikrein-like serine protease that is 80% similar to f-PSA. The proposed method was finally applied to determine f-PSA in 40 samples of human sera. Results obtained using the method showed high correlation with those obtained using a commercially available microplate CLEIA kit (correlation coefficient, 0.9821). This strategy shows great potential application in the fabrication of diagnostic kits for determining f-PSA in serum. PMID:24139579

Liu, Ruping; Wang, Cheng; Jiang, Quan; Zhang, Wei; Yue, Zhao; Liu, Guohua

2013-11-01

359

Single-cell chemiluminescence imaging of parvovirus B19 life cycle.  

PubMed

Human parvovirus B19 (B19V) is a single-stranded DNA virus. The genome encodes a multifunctional non-structural protein (NS), two capsid proteins (VP1, VP2) and other small non-structural proteins (7.5 kDa, 9 kDa, 11 kDa). Within sensitive cells, B19V can achieve a productive replicative cycle or, on the contrary, establish persistence; differences in its expression profile have been yet investigated following in vitro infections by methodologies enabling information on the entire infected cell population. Conversely, the present study reports quantitative data on the production of B19V analytes (DNA, RNAs, and proteins) at single cell-level, underlining cell-to-cell differences through the viral specific macromolecular synthesis process. Microscope imaging assays (in situ hybridization and immunocytochemical assays), exploiting chemiluminescence as principle detection and targeting viral nucleic acids and antigens, have been performed on a permissive cell line following in vitro infection. Chemiluminescence, involving the emission of photons deriving from a chemical reaction, provided the localization and quantitative detection of analytes down to a few molecules within infected cells. In our experimental conditions, B19V transcriptional activity, leading to the production of NS and VP RNAs, has been detected early in the viral cycle (from 12h post-infection, hpi) and before genome replication, starting at 24 hpi. The analysis of VP RNAs and related proteins suggested an inhibitory effect on capsid protein translation, as a post-transcriptional regulation events. Indeed, high levels of VP transcripts have been detected at early stages of infection while the proteins accumulated within cells only at 48-72 hpi. PMID:24100233

Bonvicini, Francesca; Mirasoli, Mara; Manaresi, Elisabetta; Bua, Gloria; Calabria, Donato; Roda, Aldo; Gallinella, Giorgio

2013-12-26

360

Determination of lipoic acid by flow-injection and high-performance liquid chromatography with chemiluminescence detection.  

PubMed

A new flow-injection (FI) and high performance liquid chromatography (HPLC) with chemiluminescence detection method has been proposed for the determination of ?-lipoic acid (LA). The assay is based on the measurement of chemiluminescence (CL) produced during the reaction of ?-lipoic acid with potassium permanganate in a sodium hexametaphosphate medium (pH 3). This reaction is accompanied by a weak CL, which is greatly increased in the presence of a formaldehyde solution. The proposed FI method allows the determination of LA over the range: 0.5-20?gmL(-1) with LOD 4×10(-3)?gmL(-1). An introduction of HPLC into the flow manifold improves selectivity of the method and allows the determination of LA in a complex sample. The chromatographic linear range is 2.5-30?gmL(-1) with LOD 1.774?gmL(-1). Chromatographic separation was achieved by isocratic elution (acetonitrile/potassium dihydrogen phosphate, pH 3, adjusted with phosphoric acid): 30/70 using a Cosmosil 5C(18)-MS-II (4.6mm×150mm I.D.) column at a flow rate of 1.0mLmin(-1). The presented methods were utilized to determine the ?-lipoic acid content in "Alfa-lipoic acid" capsules and in food products. PMID:22817954

Wo?yniec, E; Karpi?ska, J; Losiewska, S; Turkowicz, M; Klimczuk, J; Koj?o, A

2012-07-15

361

Electronic excitations by chemical reactions on metal surfaces  

Microsoft Academic Search

Dissipation of chemical energy released in exothermic reactions at metal surfaces may happen adiabatically by creation of phonons or non-adiabatically by excitation of the electronic system of the metal or the reactants. In the past decades, the only direct experimental evidence for such non-adiabatic reactions has been exoelectron emission into vacuum and surface chemiluminescence which are observed in a special

Hermann Nienhaus

2002-01-01

362

Flow injection chemiluminescence determination of hydrazine by oxidation with chlorinated isocyanurates.  

PubMed

A rapid and sensitive flow injection chemiluminescence (CL) method is described for the determination of hydrazine based on the CL generated during its reaction with either sodium dichloroisocyanurate (SDCC) or trichloroisocyanuric acid (TCCA) in alkaline medium. The emission intensity is greatly enhanced if dichlorofluorescein (DCF) as sensitizer is present in the reaction medium. The presence of citrate prevents the precipitation of some cations in the reaction medium and also causes an enhancement in emission intensity. The effect of analytical and flow injection variables on these CL systems and determination of hydrazine are discussed. The optimum parameters for the determination of hydrazine were studied and were found to be the following: SDCC and TCCA both 1x10(-3) M; NaOH, 2x10(-1) M; DCF, 5x10(-6) M; citrate, 1x10(-3) M and flow rate, 3.8 ml min(-1). The optimized method yielded 3sigma detection limits of 2x10(-7) and 3x10(-7) M for hydrazine with SDCC and TCCA oxidants, respectively. The method is simple, fast, sensitive, and precise and was applied to the determination of hydrazine in water samples. PMID:18968808

Safavi, Afsaneh; Karimi, Mohammad Ali

2002-10-16

363

Low-level chemiluminescence of hydroperoxide-supplemented cytochrome c  

PubMed Central

Ferricytochrome c showed low-level chemiluminescence, with a light-emission measured of about 1×103–3×103 counts/s, when supplemented with organic hydroperoxides. Tertiary hydroperoxides (cumene hydroperoxide and t-butyl hydroperoxide) showed a saturation behaviour at about 5mm-hydroperoxide, whereas primary hydroperoxides showed a quadratic dependence on the hydroperoxide concentration. Chemiluminescence depended linearly on cytochrome c concentration, and optimal light-emission was observed at [t-butyl hydroperoxide]/[ferricytochrome c] ratios of 160–500. Hydroperoxide-supplemented ferricytochrome c consumed O2 at a rate of 1.0?mol/min per ?mol of cytochrome c; the rate of O2 uptake was linearly related to the concentration of cytochrome c. The Soret absorption band of ferricytochrome c decreased about 64% after incubation with t-butyl hydroperoxide, whereas the 530nm band was almost totally abolished. Light-emission was (a) inhibited competitively by cyanide. (b) inhibited by singlet-oxygen quenchers (e.g. ?-carotene), scavengers (e.g. dimethylfuran) and traps (e.g. histidine and tryptophan) and (c) increased by singlet-oxygen-chemiluminescence enhancer 1,4-diazabicyclo[2.2.2]-octane. Superoxide dismutase had no effect on the present system. The participation of free radicals is suggested by the effect of the radical trap 2,5-di-t-butylquinol. Singlet-oxygen dimol emission seems to be mainly responsible for the observed light-emission; a mechanism that can account for the major part of the present experimental observations is proposed.

Cadenas, Enrique; Boveris, Alberto; Chance, Britton

1980-01-01

364

Electrogenerated chemiluminescence from PbS quantum dots.  

PubMed

We report the first observation of electrogenerated chemiluminescence (ECL) from PbS quantum dots (QDs). Different ECL intensities are observed for different ligands used to passivate the QDs, which indicates that ECL is sensitive to surface chemistry, with the potential to serve as a powerful probe of surface states and charge transfer dynamics in QDs. In particular, passivation of the QD surfaces with trioctylphosphine (TOP) increases ECL intensity by 3 orders of magnitude when compared to passivation with oleic acid alone. The observed overlap of the ECL and photoluminescence spectra suggests a significant reduction of deep surface trap states from the QDs passivated with TOP. PMID:19115964

Sun, Liangfeng; Bao, Lei; Hyun, Byung-Ryool; Bartnik, Adam C; Zhong, Yu-Wu; Reed, Jason C; Pang, Dai-Wen; Abruña, Héctor D; Malliaras, George G; Wise, Frank W

2009-02-01

365

ENA screen chemiluminescence immunoassay: a random access method in autoimmunology.  

PubMed

Recently, autoimmunity, due to an increase in examination requests, has become an independent area of laboratory research, which needs management optimization in terms of quality, time, and flexibility. Therefore, we have evaluated the screening of extractable nuclear antigens (ENA) antibodies both with a chemiluminescence immunoassay and the enzyme-linked immunosorbent assay (ELISA) method, which was used in our laboratory, as a reference kit. The most important difference between these two methods is the possibility of processing serum samples with a random access system, which is different from batch methods. PMID:17785312

Signorini, S; Kutschera, I; Capuano, F; Lattuada, L

2007-08-01

366

Electrogenerated chemiluminescence properties of bisalicylideneethylenediamino (salen) metal complexes  

Microsoft Academic Search

The spectroscopy, electrochemistry and electrogenerated chemiluminescence (ECL) of eight bisalicylideneethylenediamino (salen) metal complexes are reported. Two of the complexes contain an unsubstituted salen ligand and either cobalt(II) or nickel(II). The others have 1,2-cyclohexanediamonio-N,N?-bis(3,5-di-t-butylsalicylidene) as the ligand, and chromium(III), aluminum(III), cobalt(II), cobalt(III) or manganese(II) as the metal center. The complexes have lowest energy absorption maxima between 350 and 430nm. When excited

Megan Schnuriger; Eric Tague; Mark M. Richter

367

The molecular dynamics of atmospheric reaction  

NASA Technical Reports Server (NTRS)

Detailed information about the chemistry of the upper atmosphere took the form of quantitative data concerning the rate of reaction into specified states of product vibration, rotation and translation for exothermic reaction, as well as concerning the rate of reaction from specified states of reagent vibration, rotation and translation for endothermic reaction. The techniques used were variants on the infrared chemiluminescence method. Emphasis was placed on reactions that formed, and that removed, vibrationally-excited hydroxyl radicals. Fundamental studies were also performed on exothermic reactions involving hydrogen halides.

Polanyi, J. C.

1971-01-01

368

Retrovirus-Induced Oxidative Stress with Neuroimmunodegeneration Is Suppressed by Antioxidant Treatment with a Refined Monosodium ?-Luminol (Galavit)  

PubMed Central

Oxidative stress is involved in many human neuroimmunodegenerative diseases, including human immunodeficiency virus disease/AIDS. The retrovirus ts1, a mutant of Moloney murine leukemia virus, causes oxidative stress and progressive neuro- and immunopathology in mice infected soon after birth. These pathological changes include spongiform neurodegeneration, astrogliosis, thymic atrophy, and T-cell depletion. Astrocytes and thymocytes are directly infected and killed by ts1. Neurons are not infected, but they also die, most likely as an indirect result of local glial infection. Cytopathic effects of ts1 infection in cultured astrocytes are associated with accumulation of the viral envelope precursor protein gPr80env in the endoplasmic reticulum (ER), which triggers ER stress and oxidative stress. We have reported (i) that activation of the Nrf2 transcription factor and upregulation of antioxidative defenses occurs in astrocytes infected with ts1 in vitro and (ii) that some ts1-infected astrocytes survive infection by mobilization of these pathways. Here, we show that treatment with a refined monosodium ?-luminol (Galavit; GVT) suppresses oxidative stress and Nrf2 activation in cultured ts1-infected astrocytes. GVT treatment also inhibits the development of spongiform encephalopathy and gliosis in the central nervous system (CNS) in ts1-infected mice, preserves normal cytoarchitecture in the thymus, and delays paralysis, thymic atrophy, wasting, and death. GVT treatment of infected mice reduces ts1-induced oxidative stress, cell death, and pathogenesis in both the CNS and thymus of treated animals. These studies suggest that oxidative stress mediates ts1-induced neurodegeneration and T-cell loss.

Jiang, Yuhong; Scofield, Virginia L.; Yan, Mingshan; Qiang, Wenan; Liu, Na; Reid, Amy J.; Lynn, William S.; Wong, Paul K. Y.

2006-01-01

369

Neuroprotective effects of the drug GVT (monosodium luminol) is mediated by the stabilization of Nrf2 in astrocytes  

PubMed Central

Oxidative stress is implicated in various kinds of neurological disorders, including human immunodeficiency virus (HIV) associated dementia (HAD). Our laboratory has been studying the murine retrovirus ts1, a pathogenic mutant of the Moloney murine leukemia virus (MoMuLV), as a model for HAD. Like HIV in humans, ts1 induces oxidative stress and progressive neurodegeneration in mice. We have shown previously that an antioxidant and anti-inflammatory drug GVT or MSL (monosodium luminol) suppresses ts1-induced oxidative stress, attenuates the development of spoorm encephalopathy, and delays hind limb paralysis in infected mice. It is known that upregulation of the nuclear transcription factor NF-E2-related factor 2 (Nrf2) is involved in upregulating cellular antioxidant defenses. Since Nrf2 is associated with elevation of antioxidant defenses in general, and since GVT suppresses ts1-induced neurodegeneration, our aim in this study was to determine whether GVT neuroprotection is linked to Nrf2 upregulation in the brain. We report here that GVT upregulates the levels of Nrf2, both in primary astrocyte cultures and in brainstem of ts1-infected mice. Significant upregulation of Nrf2 expression by GVT occurs in both the cytosolic and nuclear fractions of cultured astrocytes and brainstem cells. Notably, although GVT treatment increases Nrf2 protein levels in cultured astrocytes and brainstem tissues, Nrf2 mRNA levels are not altered. This suggests that the neuroprotective effects of GVT may be mediated by the stabilization of the Nrf2 protein, allowing continuous upregulation of Nrf2 levels in the astrocytes.

Reddy, Pichili Vijaya Bhaskar; Lungu, Gina; Kuang, Xianghong; Stoica, George; Wong, Paul KY

2014-01-01

370

Evaluation of Single Column Trapping/Separation and Chemiluminescence Detection for Measurement of Methanethiol and Dimethyl Sulfide from Pig Production  

PubMed Central

Reduced sulfur compounds are considered to be important odorants from pig production due to their low odor threshold values and low solubility in slurry. The objective of the present study was to investigate the use of a portable method with a single silica gel column for trapping/separation coupled with chemiluminescence detection (SCTS-CL) for measurement of methanethiol and dimethyl sulfide in sample air from pig production. Proton-transfer-reaction mass spectrometry (PTR-MS) was used to evaluate the trapping/separation. The silica gel column used for the SCTS-CL efficiently collected hydrogen sulfide, methanethiol and dimethyl sulfide. The measurement of methanethiol by SCTS-CL was clearly interfered by the high concentration of hydrogen sulfide found in pig production, and a removal of hydrogen sulfide was necessary to obtain reliable results. Air samples taken from a facility with growing-finishing pigs were analyzed by SCTS-CL, PTR-MS, and a gas chromatograph with sulfur chemiluminescence detection (GC-SCD) to evaluate the SCTS-CL. The difference between the concentrations of methanethiol and dimethyl sulfide measured with SCTS-CL, PTR-MS, and GC-SCD was below 10%. In conclusion, the SCTS-CL is a portable and low-cost alternative to the commercial methods that can be used to measure methanethiol and dimethyl sulfide in sample air from pig production.

Hansen, Michael J?rgen; Toda, Kei; Obata, Tomoaki; Adamsen, Anders Peter S.; Feilberg, Anders

2012-01-01

371

Evaluation of single column trapping/separation and chemiluminescence detection for measurement of methanethiol and dimethyl sulfide from pig production.  

PubMed

Reduced sulfur compounds are considered to be important odorants from pig production due to their low odor threshold values and low solubility in slurry. The objective of the present study was to investigate the use of a portable method with a single silica gel column for trapping/separation coupled with chemiluminescence detection (SCTS-CL) for measurement of methanethiol and dimethyl sulfide in sample air from pig production. Proton-transfer-reaction mass spectrometry (PTR-MS) was used to evaluate the trapping/separation. The silica gel column used for the SCTS-CL efficiently collected hydrogen sulfide, methanethiol and dimethyl sulfide. The measurement of methanethiol by SCTS-CL was clearly interfered by the high concentration of hydrogen sulfide found in pig production, and a removal of hydrogen sulfide was necessary to obtain reliable results. Air samples taken from a facility with growing-finishing pigs were analyzed by SCTS-CL, PTR-MS, and a gas chromatograph with sulfur chemiluminescence detection (GC-SCD) to evaluate the SCTS-CL. The difference between the concentrations of methanethiol and dimethyl sulfide measured with SCTS-CL, PTR-MS, and GC-SCD was below 10%. In conclusion, the SCTS-CL is a portable and low-cost alternative to the commercial methods that can be used to measure methanethiol and dimethyl sulfide in sample air from pig production. PMID:22997603

Hansen, Michael Jørgen; Toda, Kei; Obata, Tomoaki; Adamsen, Anders Peter S; Feilberg, Anders

2012-01-01

372

Quantitative analysis of hydroperoxyl radical using flow injection analysis with chemiluminescence detection.  

PubMed

The hydroperoxyl radical (HO2) is one of the most abundant free radicals in the atmosphere, where it participates in a series of photochemical reactions that determine the fate of natural and anthropogenic emissions. In addition, HO2 is found in droplets and surface water as a result of photochemical formation and gas-phase scavenging. We describe a quantitative method for determining trace concentrations of HO2 radicals that exploits the chemiluminescence produced upon reaction with a synthetic analogue of luciferin from the crustacean Cypridina. The technique is linear at least up to 1 microM HO2(aq) and has a minimum detection limit of 0.1 nM. A unique feature of this analysis is a calibration method using stable aqueous HO2 standards produced in submicromolar concentrations using 60Co gamma-radiolysis. The advantage of this method in comparison to others in consideration of field deployment is its simplicity, low cost, and minimal size and power requirements. One intended application of this technique is the measurement of atmospheric HO2 radicals following collection into aqueous solution. PMID:14632084

Zheng, Jun; Springston, Stephen R; Weinstein-Lloyd, Judith

2003-09-01

373

Flow-injection chemiluminescence determination of chrysin and baicalein assisted by theoretical prediction of chemiluminescence behavior of chrysin and baicalein  

NASA Astrophysics Data System (ADS)

In this paper, the molecular connectivity indices were applied to theoretically predict the direct chemiluminescence (CL) behavior of chrysin and baicalein with our recently proposed discriminant function. Then, combined with flow-injection analysis, a new CL system for determination of chrysin and baicalein was proposed. The method was based on the oxidation of chrysin and baicalein by acidic KMnO 4 in the presence of formaldehyde to produce strong CL emission. The present paper suggested a new model to discover new CL analytical system: first, to theoretical predict the CL behavior, and the second, to suggest analytical system.

Li, Baoxin; Guo, Lili; Xu, Chunli; Ma, Limei

2008-12-01

374

Lucigenin-derived chemiluminescence in intact isolated mitochondria.  

PubMed

There are many data both in favor and against the use of lucigenin as a probe for superoxide anion (SA) in mitochondria, cells, and simple enzymatic systems. In the present work high concentrations (50-400 micro M) of lucigenin were used for continuous recording of rapid and reversible changes in the SA level in intact isolated mitochondria. The SA level in the presence of lucigenin rapidly and reversibly changed during the transition of the mitochondria from one functional state to another: under conditions of ATP synthesis from ADP and Pi, of Ca2+ accumulation, and of reverse electron transfer. Induction of a Ca2+,cyclosporin A-sensitive pore in mitochondria completely suppressed the lucigenin-derived chemiluminescence (LDC). The electron transfer in the Q-cycle of the respiratory chain complex III and high electric potential difference across the inner membrane of mitochondria were obligatory conditions for generation of a SA-dependent chemiluminescent signal. Based on our own and literature data, a scheme of LDC generation is suggested. The origin of superoxide anion detected in intact mitochondria with lucigenin is discussed. PMID:12495424

Kruglov, A G; Yurkov, I S; Teplova, V V; Evtodienko, Yu V

2002-11-01

375

Synthesis of Chemiluminescent Esters: A Combinatorial Synthesis Experiment for Organic Chemistry Students  

ERIC Educational Resources Information Center

A group of techniques aimed at synthesizing a large number of structurally diverse compounds is called combinatorial synthesis. Synthesis of chemiluminescence esters using parallel combinatorial synthesis and mix-and-split combinatorial synthesis is experimented.

Duarte, Robert; Nielson, Janne T.; Dragojlovic, Veljko

2004-01-01

376

Experimental and Theoretical Study of Chemiluminescence in Flames and of Combustion in Solid Propellants.  

National Technical Information Service (NTIS)

The two parts of the report deal successively with chemiluminescence in flames and with combustion of solid propellants. Relationships between chemiluminiscence (CL) and chemi-ionization (CI) on one hand, and the molecular structure of the fuel on the oth...

P. J. Van Teggelen

1970-01-01

377

A comparison of chemiluminescent and colorimetric substrates in a hepatitis B virus DNA hybridization assay.  

PubMed

We compared the sensitivity of a chemiluminescent substrate 3-(2'-spiroadamantane)-4-methoxy-4-(3"-phosphoryloxy)phenyl- 1,2-dioxetane (AMPPD) and a chromogenic substrate 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) for detection of an alkaline phosphatase label in a hepatitis B virus "core antigen" DNA (HBVc) probe hybridization assay. Chemiluminescent signal obtained from AMPPD hydrolysis by alkaline phosphatase was detected with Polaroid Instant Black and White Type 612 film. The chemiluminescent assay detected 1.18 x 10(6) copies of HBVc plasmid DNA in 30 min. By comparison, 9.8 x 10(7) copies of DNA could be measured using chromogenic BCIP/NBT substrate within the same incubation time. After further development, the chemiluminescent endpoint permitted detection of 4.39 x 10(4) copies of HBVc plasmid DNA in 2 h. PMID:2817349

Bronstein, I; Voyta, J C; Edwards, B

1989-07-01

378

First Ozone Profiles Measured with Electrochemical and Chemiluminescent Sondes, Developed in Russia.  

National Technical Information Service (NTIS)

Results obtained with experimental balloon electrochemical and chemiluminescent ozonesondes are summarized and estimated as quite satisfactory. The average normalization factor for the electrochemical ozonesonde obtained in 1991 at four Soviet balloon rou...

A. M. Zuyaguintsev S. P. Perov Y. A. Ryabov

1994-01-01

379

Suitability of chemiluminescent enzyme immunoassay for the measurement of blood tacrolimus concentrations in rheumatoid arthritis  

Microsoft Academic Search

ObjectivesThe aim of this study was to evaluate the suitability of chemiluminescent enzyme immunoassay (CLIA) for the monitoring of whole-blood tacrolimus concentrations in rheumatoid arthritis (RA) patients.

Kumi Hirano; Shuji Maruyama; Yasuaki Mino; Takafumi Naito; Junichi Kawakami

2011-01-01

380

SO 32--based chemiluminescence in unbuffered solution with ClO 2 as oxidant and its analytical application  

NASA Astrophysics Data System (ADS)

In this paper, SO 32--chemiluminescence (CL) system in unbuffered solution with ClO 2 as oxidant is proposed. ClO 2 could oxidize sulfite in unbuffered solution to produce CL emission, and riboflavin could sensitize the ClO 2-SO 32--based CL system. The ClO 2-SO 32--riboflavin CL reaction was chosen as a model system and explored the possibility of SO 32--based CL system in unbuffered solution. Compared with the reported SO 32--based CL system in strong acid media, the proposed CL system owns its advantages. Combined with flow-injection analysis, the proposed CL system was applied to measurement of riboflavin in pharmaceuticals.

Li, Baoxin; Deng, Qingwen; Xu, Chunli

2007-11-01

381

Feasibility study for rocket ozone measurements in the 50 to 80 km region using a chemiluminescent technique  

NASA Technical Reports Server (NTRS)

A study has been conducted to determine the feasibility of increasing sensitivity for ozone detection. The detection technique employed is the chemiluminescent reaction of ozone with a rhodamine-B impregnated disk. Previously achieved sensitivities are required to be increased by a factor of about 20 to permit measurements at altitudes of 80 km. Sensitivity was increased by using a more sensitive photomultiplier tube, by increasing the gas velocity past the disk, by different disk preparation techniques, and by using reflective coatings in the disk chamber and on the uncoated side of the glass disk. Reflective coatings provided the largest sensitivity increase. The sum of all these changes was a sensitivity increased by an estimated factor of 70, more than sufficient to permit measurement of ambient ozone concentrations at altitudes of 80 km.

Goodman, P.

1973-01-01

382

Chemiluminescent immunoenzymatic assay for rapid diagnosis of viral infections.  

PubMed Central

Current methods of viral diagnosis have been criticized for slowness and insensitivity. However, immunoassay may provide the desired increase in the speed of diagnosis without sacrificing accuracy. This report describes the efficacy of the detection of viral antigen by means of an absorptiometric enzyme-linked immunoassay (ELISA) or by a chemiluminescent enzyme-linked immunoassay (CELISA). Human cytomegalovirus was detected in clinical specimens or culture fluid with comparable sensitivity by CELISA and by viral isolation but with 50 times lesser sensitivity by ELISA. Similarly, herpes simplex virus was detected in clinical specimens with markedly greater sensitivity by CELISA than by ELISA. These findings indicate that the detection of appropriate viruses by CELISA may be a practical alternative to their isolation in cell culture.

Pronovost, A D; Baumgarten, A; Andiman, W A

1982-01-01

383

Sonochemical synthesis of Ag nanoclusters: electrogenerated chemiluminescence determination of dopamine.  

PubMed

We report a facile one-pot sonochemical approach to preparing highly water-soluble Ag nanoclusters (NCs) using bovine serum albumin as a stabilizing agent and reducing agent in aqueous solution. Intensive electrogenerated chemiluminescence (ECL) was observed from the as-prepared Ag (NCs) and successfully applied for the ECL detection of dopamine with high sensitivity and a wide detection range. A possible ECL mechanism is proposed for the preparation of Ag NCs. With this method, the dopamine concentration was determined in the range of 8.3 × 10(-9) to 8.3 × 10(-7) mol/L without the obvious interference of uric acid, ascorbic acid and some other neurotransmitters, such as serotonin, epinephrine and norepinephrine, and the detection limit was 9.2 × 10(-10) mol/L at a signal/noise ratio of 3. PMID:23418144

Liu, Tao; Zhang, Lichun; Song, Hongjie; Wang, Zhonghui; Lv, Yi

2013-01-01

384

Determination of theophylline concentration in serum by chemiluminescent immunoassay  

PubMed Central

Objective: This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum. Methods: To measure the concentration of theophylline (n=122) and evaluate the assay. Results: The linear range of the CLIA method was 0.51~40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 ?mol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L). Conclusion: This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.

Zhou, Mei-xia; Guan, Cha-ying; Chen, Guang; Xie, Xin-you; Wu, Sheng-hai

2005-01-01

385

Chemiluminescence as diagnostic tool in the development of gas turbines  

NASA Astrophysics Data System (ADS)

To optimise the operation of gas turbine combustors with respect to emission, cycle efficiency and components lifetime, increased attention has to be attributed to diagnostic techniques and more flexible control schemes. Chemiluminescence is an obvious choice and a relatively easy and low cost option for such a diagnostic tool. Application examples include spectral analysis and light intensity scaling, temporal analysis studying flame dynamic effects and imaging techniques resolving spatial distribution of heat release zones, as well as combinations of the methods like phase matched imaging and tracking of ignition kernels using high speed imaging. Further fundamental work should be triggered on the nature for the excited species and their formation pathways as well as their connection to heat release and the NO x formation processes.

Guethe, F.; Guyot, D.; Singla, G.; Noiray, N.; Schuermans, B.

2012-06-01

386

Chemiluminescence from the decomposition of peroxymonocarbonate catalyzed by gold nanoparticles.  

PubMed

In this work, an online preparation of peroxymonocarbonate was formed innovatively, which offered the reliable intermediate for further investigation. Gold colloids with nanoparticles of different sizes were found to enhance the chemiluminescence (CL) of the peroxymonocarbonate-eosin Y system, and the most intensive CL signals were obtained with 50 +/- 1 nm diameter gold nanoparticles. UV-visible adsorption spectra, fluorescence spectra, transmission electron microscopy images, electron paramagnetic resonance spin-trapping spectra, and mass spectra were obtained in order to study the CL enhancement mechanism. Peroxymonocarbonate, a reactive oxygen species, can be decomposed to singlet oxygen which transfers its energy to eosin Y. The CL can be induced by excited eosin Y. Gold nanoparticles facilitated the radical generation and singlet oxygen molecular formation on the surface of the gold nanoparticles. Thus, the CL emission enhanced greatly by adding gold nanoparticles into the system. PMID:18543872

Lin, Jin-Ming; Liu, Meilin

2008-07-01

387

Chemiluminescence enzyme immunoassay for the determination of sulfamethoxydiazine.  

PubMed

Sulfamethoxydiazine (SMD), which is often used for animal disease treatment, is harmful to human health. No SMD residue should be detected in food in some countries, such as USA and Japan. Therefore, it is significant to develop a high-throughput, high-sensitivity and accurate method for the determination of the content of SMD in food. In this paper, chemiluminescence enzyme immunoassay (CLEIA) was developed for quantification of SMD. For this method, the limit of detection was 3.2 pg/ml, the linear range was from 10 to 2000 pg/ml, the within-day and inter-day precision were below 13% and below 18%, respectively, and the recovery was from 85% to 105%. Milk and egg were selected as samples to be examined with this method, and the result indicated that this CLEIA method was suitable for screening and quality control of food. PMID:21795101

Wu, Yongjun; Yu, Songcheng; Yu, Fei; Yan, Nali; Qu, Lingbo; Zhang, Hongquan

2011-10-15

388

Ester oxidation on an aluminum surface using chemiluminescence  

NASA Technical Reports Server (NTRS)

The oxidation characteristics of a pure ester (trimethyolpropane triheptanoate) were studied by using a chemiluminescence technique. Tests were run in a thin film microoxidation apparatus with an aluminum alloy catalyst. Conditions included a pure oxygen atmosphere and a temperature range of 176 to 206 C. Results indicated that oxidation of the ester (containing .001 M diphenylanthracene as an intensifier) was accompanied by emission of light. The maximum intensity of light emission was a function of the amount of ester, the concentration of intensifier, and the test temperature. The induction period, or the time to reach one-half of maximum intensity was inversely proportional to test temperature. Decreases in light emission at the later stages of a test were caused by depletion of the intensifier.

Jones, William R., Jr.; Meador, Michael A.; Morales, Wilfredo

1986-01-01

389

Chemiluminescence enzyme immunoassay for the determination of sulfamethoxydiazine  

NASA Astrophysics Data System (ADS)

Sulfamethoxydiazine (SMD), which is often used for animal disease treatment, is harmful to human health. No SMD residue should be detected in food in some countries, such as USA and Japan. Therefore, it is significant to develop a high-throughput, high-sensitivity and accurate method for the determination of the content of SMD in food. In this paper, chemiluminescence enzyme immunoassay (CLEIA) was developed for quantification of SMD. For this method, the limit of detection was 3.2 pg/ml, the linear range was from 10 to 2000 pg/ml, the within-day and inter-day precision were below 13% and below 18%, respectively, and the recovery was from 85% to 105%. Milk and egg were selected as samples to be examined with this method, and the result indicated that this CLEIA method was suitable for screening and quality control of food.

Wu, Yongjun; Yu, Songcheng; Yu, Fei; Yan, Nali; Qu, Lingbo; Zhang, Hongquan

2011-10-01

390

Chemiluminescent nitrogen detection (CLND) to measure kinetic aqueous solubility.  

PubMed

Solubility is the dose-limiting property for in vitro studies, and therefore is a critical physicochemical property to measure in drug discovery. Solubility data can be used to guide lead optimization, troubleshoot erratic bioassay results, and identify potential downstream liabilities such as insufficient solubility for bioassays or oral bioavailability. Typically, early in vitro studies are performed using library compounds prepared as dimethylsulfoxide (DMSO) stock solutions, resulting in in vitro test solutions containing DMSO at low concentration (<5% v/v). Since DMSO can affect the apparent solubility, it is desirable to obtain solubility data under conditions mimicking the in vitro study. Kinetic solubility (from DMSO stock solutions) is often preferred over thermodynamic solubility (from dry powder) in early drug discovery. The protocols in this article describe a general procedure for assessing kinetic aqueous solubility of early drug discovery compounds using a miniaturized shake flask method with chemiluminescent nitrogen detection (CLND). PMID:24391097

Kestranek, Aimee; Chervenak, Andrew; Longenberger, Justin; Placko, Steven

2013-12-01

391

Determination of riboflavin by enhancing the chemiluminescence intensity of peroxomonosulfate-cobalt(II) system  

NASA Astrophysics Data System (ADS)

A weak chemiluminescent (CL) emission was observed in the decomposition of peroxomonosulfate (HSO 5-), which would be accelerated in the presence of trace amounts of cobalt (II). The mechanism was due to the production of singlet oxygen ( 1O 2). Interestedly, riboflavin can enhance the CL and the CL intensity was strongly dependent on riboflavin concentration. Based on this phenomenon, a flow injection analysis (FIA) CL method was established for the determination of riboflavin. Additionally, the possible CL mechanism is proposed based on the kinetic curve of the CL reaction, CL spectra, UV-vis spectra and fluorescent spectra. The CL intensity was correlated linearly with concentration of riboflavin over the range of 1.0 × 10 -4 to 1.0 × 10 -8 g mL -1; the detection limit was 9.0 × 10 -9 g mL -1(S/N = 3); the relative standard deviation was 1.4% for 9 × 10 -7 g mL -1 riboflavin ( n = 11). Furthermore, this method was applied to the determination of riboflavin in real tablets and injections successfully.

Wang, Min; Zhao, Lixia; Liu, Meilin; Lin, Jin-Ming

2007-04-01

392

Iron stimulation of chemiluminescence by microsomes and purified NADPH-cytochrome P-450 reductase  

SciTech Connect

Low level chemiluminescence (CL) was measured as an assay of the steady state level of production of oxygen radicals during microsomal electron transfer. In the presence of an NADPH-generating system, antioxidant-sensitive CL was observed with isolated rat liver microsomes. Depending on the nature of the chelating agent, ferric iron markedly affected this CL. For example, ferric-EDTA inhibited, whereas ferric-ADP stimulated CL. This response to iron chelators was identical to that found when measuring microsomal lipid peroxidation, but was opposite to the catalytic effectiveness of ferric-chelates in stimulating microsomal generation of hydroxyl radicals. Similar studies were conducted with purified NADPH-cytochrome P-450 reductase in the presence of t-butyl hydroperoxide (t-BOOH). No CL was observed in the absence of added iron. The addition of ferric-EDTA or ferric-detapac stimulated production of CL, whereas ferric chloride or ferric-ATP has little or no effect. This pattern of response to iron chelates is opposite to that found with the microsomes. CL was inhibited by catalase and OH scavengers such as ethanol and DMSO but not by superoxide dismutase. Thus, CL by the reductase system appears to involve the generation of OH via a Fenton-type of reaction, and subsequent interaction of OH with t-BOOH to produce excited species.

Puntarulo, S.; Clejan, L.; Palakodety, R.; Cederbaum, A.I.

1987-05-01

393

A high throughput chemiluminescence method for determination of chemical oxygen demand in waters.  

PubMed

A novel and high throughput chemiluminescence (CL) method for determination of chemical oxygen demand (COD) in water sample was originally developed based on potassium permanganate-glutaraldehyde CL system. With this method, dissolved organic matter in water samples was digested by excess acid potassium permanganate, the reacted mixture solutions containing surplus KMnO(4) were added in wells of a 96-well plate, followed by injection of glutaraldehyde in the wells, and CL was then produced along with the reaction of the added glutaraldehyde with the surplus KMnO(4) and detected by a photomultiplier tube (PMT). The difference (DeltaI) between the CL intensity for distilled water and that for sample water was proportional to the COD value of water sample. The calibration graph was linear in the range of 0.16-19.24 mg L(-1) with a detection limit of 0.1 mg L(-1). A complete analysis could be performed in 40 min including digestion and detection, giving a very high throughput of 3 x 96 samples in about 60 min. Compared with the conventional methods, this method is simple and sensitive and consumes very limited and cheap reagents. Owing to its rapid, automatic, high throughput and low cost characteristics, the presented CL method has been applied successfully to the determination of COD in real water samples (n=32) with satisfactory results. PMID:19110119

Yao, Hong; Wu, Bin; Qu, Haibin; Cheng, Yiyu

2009-02-01

394

Chemiluminescence arising from the decomposition of peroxymonocarbonate and enhanced by CdTe quantum dots.  

PubMed

CdTe quantum dots (QDs) capped with mercaptoacetic acid were applied to the hydrogen peroxide-sodium hydrogen carbonate chemiluminescence (CL) system. The CL emission intensity was significantly enhanced by different sizes of CdTe QDs. Peroxymonocarbonate (HCO(4)(-)) was formed in the reaction of hydrogen peroxide and sodium hydrogen carbonate, which was a reactive oxygen species. Decomposition of HCO(4)(-) generated superoxide ion radical (·O(2)(-)) and hydroxide radical (·OH). The enhanced CL was induced by the excited CdTe QDs, which could be produced from the combination of hole (oxidized QDs (h(+))) and electron (reduced QDs (e(-))) injected QDs. Radical scavengers and organic reagents such as nitro blue tetrazolium chloride (NBT), cytochrome c, sodium azide, ascorbic acid, thiourea, tert-butanol, and dimethyl sulphoxide were used to study the emitting species. The intermediate hydroxide radical and superoxide ion were key species for producing hole and electron-injected QDs. Four emitters such as (1)O(2), (O(2))(2)*, (CO(2))(2)* and CdTe* were detected in the CL system. The mechanism was discussed based on the CL emission spectra, electron spin resonance spectra, fluorescence spectra, and UV-vis absorption spectra. The CL properties of CdTe QDs will be helpful to study semiconductor nanocrystals and will open new avenues for the application of QDs in many fields, such as chemistry, biology, microbiology, and biochemistry. PMID:20738136

Chen, Hui; Lin, Ling; Lin, Zhen; Guo, Guangsheng; Lin, Jin-Ming

2010-09-23

395

Singlet oxygen generated from the decomposition of peroxymonocarbonate and its observation with chemiluminescence method.  

PubMed

The decomposition of peroxymonocarbonate (HCO(4)(-)) has been investigated by flow-injection chemiluminescence (CL) method. An ultraweak CL was observed during mixing the bicarbonate and hydrogen peroxide solution in organic cosolvent. An appropriate amount of fluorescent organic compounds, such as dichlorofluorescein (DCF), was added to the HCO(4)(-) solution, a strong CL was recorded. Based on studies of the spectrum of fluorescence, CL and UV-vis spectra, electron spin trapping (ESR) technique, mass spectra (MS) and comparison with H(2)O(2)/hypochlorite (ClO(-)) and H(2)O(2)/molybdate (MoO(4)(-)) systems, the CL mechanism was proposed. The reaction is initiated by unimolecular homolysis of the peroxo O-O bond in HO-OCOO(-) molecule. It was suggested that the bond rearrangement within radicals yield superoxide ion (O(2)(*-)). The interaction of superoxide ion with perhydroxyl radical produces singlet oxygen ((1)O(2)). The energy transfers from singlet oxygen to DCF forming an excited energy acceptor (DCF*). Luminescence (lambda(max)=509 nm) was emitted during the relaxation of the energy acceptor to the ground state. PMID:18996044

Lin, Jin-Ming; Liu, Meilin

2009-02-01

396

A chemiluminescence-based continuous flow aqueous ozone analyzer using photoactivated chromotropic acid.  

PubMed

Ozone has become the oxidant of choice for water disinfection, especially in large water treatment facilities. This paper describes a fast and sensitive method for the determination of ozone content by reaction with photoactivated chromotropic acid (CA, 4,5-dihydroxynaphthalene-2,7-disulfonic acid), which results in intense chemiluminescence (CL). Freshly ozonated water from a recirculating ozonizer/reservoir is injected into a carrier stream of deionized water in the flow-injection mode. This flow mixes with a stream of photoactivated CA solution in a spiral cell placed directly on top of an inexpensive miniature (8mm diameter active area) photomultiplier tube (PMT). Alkaline CA is photoactivated by passing it through a FEP-Teflon((R)) coil (residence time approximately 50s) wrapped around a 1W UV lamp emitting at 254nm; without photoactivation, the signal is approximately 70-fold lower. The S/N=3 limit of detection for aqueous ozone is 3mugl(-1) and good response slope is obtained up to an ozone concentration of 1.4mgl(-1), the highest that could be made in this study. The response obeyed a quadratic equation with r(2)=0.9984. No interference from permanganate ion is observed. The proposed system was applied to the monitoring of ozonation status of a playa lake water that exhibited significant ozone demand. PMID:18970059

Takayanagi, Toshio; Dasgupta, Purnendu K

2005-05-15

397

A High Sensitivity Micro Format Chemiluminescence Enzyme Inhibition Assay for Determination of Hg(II)  

PubMed Central

A highly sensitive and specific enzyme inhibition assay based on alcohol oxidase (AlOx) and horseradish peroxidase (HRP) for determination of mercury Hg(II) in water samples has been presented. This article describes the optimization and miniaturization of an enzymatic assay using a chemiluminescence reaction. The analytical performance and detection limit for determination of Hg(II) was optimized in 96 well plates and further extended to 384 well plates with a 10-fold reduction in assay volume. Inhibition of the enzyme activity by dissolved Hg(II) was found to be linear in the range 5–500 pg·mL?1 with 3% CV in inter-batch assay. Due to miniaturization of assay in 384 well plates, Hg(II) was measurable as low as 1 pg·mL?1 within 15 min. About 10-fold more specificity of the developed assay for Hg(II) analysis was confirmed by challenging with interfering divalent metal ions such as cadmium Cd(II) and lead Pb(II). Using the proposed assay we could successfully demonstrate that in a composite mixture of Hg(II), Cd(II) and Pb(II), inhibition by each metal ion is significantly enhanced in the presence of the others. Applicability of the proposed assay for the determination of the Hg(II) in spiked drinking and sea water resulted in recoveries ranging from 100–110.52%.

Deshpande, Kanchanmala; Mishra, Rupesh K.; Bhand, Sunil

2010-01-01

398

Carbon nanodots sensitized chemiluminescence on peroxomonosulfate-sulfite-hydrochloric acid system and its analytical applications.  

PubMed

In the present work, new water-soluble fluorescent carbon nanodots (C-dots) were prepared in a facile microwave pyrolysis approach in minutes by combining glycine and polyethylene glycol 200 (PEG 200). Transmission electron microscopy (TEM) measurements showed that the resulting C-dots had diameters of about 3 nm. (13)C NMR spectra further confirmed the presence of carbons (sp(2) and sp(3)) indicating a nanocrystalline core of the resulting C-dots with hydroxyl of PEG 200 covered outside. It was discovered that the prepared C-dots could dramatically enhance the chemiluminescence (CL) intensity of potassium peroxomonosulfate-sodium sulfite-hydrochloric acid (PSHA) reactions. UV-vis absorption and photoluminescence (PL) spectra indicated that the C-dots sensitized enhancements originated from their energy transfer and electron-transfer annihilation effects on the CL system. When the concentration of C-dots was 4×10(-5) M, and those of KHSO(5), Na(2)SO(3) and HCl were 1×10(-2) M, an excellent performance was obtained. The C-dots sensitized CL system was successfully applied to the determination of aliphatic primary amines in real water samples with satisfactory results. PMID:22967581

Zhou, Yun; Xing, Gaowa; Chen, Hui; Ogawa, Nobuaki; Lin, Jin-Ming

2012-09-15

399

Stress chemiluminescence of polymeric materials; predictive applications to the aging process  

Microsoft Academic Search

A computer-controlled stress chemiluminescence instrument has been designed and assembled. The significant result of this work is the correlation of an enhanced chemiluminescence signal in a low stress environment with the subsequent premature mechanical failure of samples of TGMDA-DDS, the most common epoxy system used as a matrix for high-performance composites. Preliminary results with cellular silicone elastomers indicate a correlation

Suzanne B. Monaco; Jeffery H. Richardson; James D. Breshears; Stanley M. Lanning; James E. Bowman; Connie M. Walkup

1982-01-01

400

Molecularly imprinted polymer-based chemiluminescence array sensor for the detection of proline  

Microsoft Academic Search

A new molecularly imprinted polymer (MIP)–chemiluminescence (CL) method has been developed for detection of proline. The molecularly imprinted polymer microspheres were synthesized using precipitation polymerization with hydroxyproline, a structural analogue, as the template. Polymer microspheres were immobilized in microtiter plates (96 wells) which selectively adsorbed the analyte (dansyl-proline). After washing, the bound fraction was quantified based on peroxyoxalate chemiluminescence (PO-CL)

Pingping Chang; Zhujun Zhang; Chunyan Yang

2010-01-01

401

Detection of Parvovirus B19 DNA in Bone Marrow Cells by Chemiluminescence In Situ Hybridization  

Microsoft Academic Search

A chemiluminescence in situ hybridization method was developed for the search of B19 parvovirus DNA in bone marrow cells, employing digoxigenin-labeled B19 DNA probes, immunoenzymatically detected with a highly sensitive 1,2-dioxetane phosphate as chemiluminescent substrate. The light emitted from the in situ- hybridized probe was analyzed and measured by a high-performance luminograph connected to an optical microscope and to a

MONICA MUSIANI; ALDO RODA; MARIALUISA ZERBINI; GIOVANNA GENTILOMI; PATRIZIA PASINI; GIORGIO GALLINELLA; ANDSIMONA VENTUROLI

402

Spatially and spectrally resolved chemiluminescence and temperature measurements in counterflow-diffusion flames  

Microsoft Academic Search

We investigated the chemiluminescence emission of and gas phase temperature of atmospheric pressure co unterflow methane\\/air and methane\\/hydrogen\\/air diffusion flames stabilized in the forward stagnation region of a porous cy- linder. Spectrograms of the chemiluminescence radiation from the known flame intermediates OH*, CH*, C2* and CO 2* were registered with an ICCD camera behind a spectrograph such that spatial resolution

A. Khartabil; M. Leschowski; C. Hecht; T. Dreier; C. Schulz

2009-01-01

403

A study of peroxyoxalate-chemiluminescence of 4,4'-bis{[4,6-bis (2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino}stilbene-2,2'-disulfonic acid-disodium salt as a novel blue fluorescer  

NASA Astrophysics Data System (ADS)

The chemiluminescence arising from the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO) with hydrogen peroxide in the presence of brightener 4,4'-bis{[4,6-bis(2-hydroxyethyl)amino-1,3,5-triazin-2-yl]amino}stilbene-2,2'-disulfonic acid-disodium salt (Triazinyl) has been studied. The influence of concentration of TCPO, hydrogen peroxide, Triazinyl, base catalysts and temperature on the resulting chemiluminescence was investigated. The kinetic parameters for the peroxyoxalate-chemiluminescence (PO-CL) of Triazinyl were evaluated from computer fitting of the resulting intensity-time plots. The activation energies, Ea, were evaluated from temperature dependence of the corresponding rise and fall rate constants.

Shamsipur, Mojtaba; Yeganeh-Faal, Ali; Chaichi, Mohammad Javad; Tajbakhsh, Mahmood; Parach, Ali

2007-03-01

404

Evaluation of UVA-induced oxidative stress using a highly sensitive chemiluminescence method  

NASA Astrophysics Data System (ADS)

Oxidative stress is mainly mediated by reactive oxygen species (ROS). Evaluation of oxidative stress is helpful for choosing an appropriate method to protect the organism from the oxidative damage. In this study, a highly sensitive and simple chemiluminescence method is presented for the evaluation of radiation-induced oxidative stress in human peripheral lymphocytes. The lymphocytes were irradiated by ultraviolet radiation (320-400nm, UVA) with different doses. The ROS generated by the lymphocytes was detected by chemiluminescence method, using a highly sensitive chemiluminescence probe 2-methyl-6-(p-methoxyphenyl)-3,7-dihydroimidazo[1,2-?] pyrazin-3-one (MCLA). The cell viability was detected with Cell Counting Kit-8 (CCK-8). The malondialdehyde (MDA), a marker of lipid peroxidation and oxidative stress, and the total antioxidant capacity (TAC), a parameter that is taken as evidence of oxidative stress, were measured too. The results show that both chemiluminescence intensity, cell mortality and MDA concentration of lymphocytes grow with the increase of UVA dose range from 0.5 to 8 J/cm2, while the TAC decreases. There exists a positive relationship between cell oxidative damage degree and the chemiluminescence intensity of lymphocytes. This highly sensitive chemiluminescence method would potentially provide an easy way to evaluate the level of UVA-induced oxidative stress readily, sensitively and rapidly

Gao, Bo; Xing, Da; Zhu, Debin

2005-02-01

405

Electrochemiluminescence polymerase chain reaction detection of genetically modified organisms  

Microsoft Academic Search

With the development of biotechnology, more and more genetically modified organisms (GMOs) have entered commercial market. Because of the safety concerns, detection and characterization of GMOs have attracted much attention recently. Electrochemiluminescence (ECL) method is a chemiluminescent (CL) reaction of species generated electrochemically on an electrode surface. It is a highly efficient and accurate detection method. In this paper, ECL

Jinfeng Liu; Da Xing; Xingyan Shen; Debin Zhu

2005-01-01

406

Reaction of fluorine and chlorine atoms with formaldehyde and deutero-formaldehyde  

SciTech Connect

The following reactions were studied by using infrared multiple photon dissociation to produce fluorine atoms, and infrared chemiluminescence to monitor the rate of reaction: Cl + H/sub 2/O ..-->.. HCl + HCO; F + H/sub 2/CO ..-->.. HF + HCO; Cl + D/sub 2/CO ..-->.. DCl + DCO; and F + D/sub 2/CO ..-->.. DF + DCO.

Nogar, N.S.; Fasano, D.M.

1983-01-01

407

Reaction of Fluorine and Chlorine Atoms with Formaldehyde and Deutero-Formaldehyde.  

National Technical Information Service (NTIS)

The following reactions were studied by using infrared multiple photon dissociation to produce fluorine atoms, and infrared chemiluminescence to monitor the rate of reaction: Cl + H sub 2 O implies HCl + HCO; F + H sub 2 CO implies HF + HCO; Cl + D sub 2 ...

N. S. Nogar D. M. Fasano

1983-01-01

408

The detailed isotope effect in the F + NH 3 AND F + ND 3 reactions  

NASA Astrophysics Data System (ADS)

Arrested relaxation infrared chemiluminescence measurements show that the reaction of fluorine atoms with ND 3 channels a much larger fraction of the available exoergicity into DF product vibration than the analogous NH 3 reaction channels into HF vibration. This result is interpreted by a mechanism involving competition between direct abstraction and formation of an intermediate complex.

Slaon, J. J.; Watson, D. G.; Williamson, J.

1980-09-01

409

Enhancing effect of alcoholic solvent on hydrosulfite-hydrogen peroxide chemiluminescence system.  

PubMed

In this work, a chemiluminescence (CL) reaction between hydrogen peroxide (H(2)O(2)) and sodium hydrosulfite (NaHSO(3)) was developed. Hydroxyl radical ((•)OH) and sulfite radical ((•)SO(3)(-)) were the main intermediates generated in the NaHSO(3)-H(2)O(2) CL system. Inhibition effects of radical scavengers such as thiourea, chloride ion, nitro blue tetrazolium chloride (NBT), and 5,5-dimethyl-1-pyrroline N-oxide (DMPO) indicated the existence of these two radicals. Singlet oxygen ((1)O(2)) and excited sulfur dioxide (SO(2)*) were emitting species involved in NaHSO(3)-H(2)O(2) CL system. (1)O(2) were confirmed by 1,4-diazobicyclo[2,2,2]octane (DABCO) and sodium azide (NaN(3)), which were specific (1)O(2) scavengers. In addition, electron spin resonance (ESR) spectra clearly show the existence of (1)O(2) and (•)OH. Alcoholic solvent, especially n-butanol, enhanced the ultraweak CL emission more than 40 times. The enhancing effect of alcoholic solvent on NaHSO(3)-H(2)O(2) CL system was ascribed to the formation of solvent cage, which can accelerate the reaction rate and protect the emitting species from quenching by water. The CL emission of the NaHSO(3)-n-butanol-H(2)O(2) system was measured by cutoff filters. The maximum wavelength was located around 490 nm, which belongs to (1)O(2). The wide peak from 400 to 600 nm is the characteristic peak of SO(2)*. PMID:22296104

Li, Ruibo; Chen, Hui; Li, Ying; Lu, Chao; Lin, Jin-Ming

2012-03-01

410

Human monocytes CD36 and CD16 are signaling molecules. Evidence from studies using antibody-induced chemiluminescence as a tool to probe signal transduction.  

PubMed Central

A panel of monoclonal antibodies (mAb) directed against human monocyte surface antigens was tested for their capacity to mediate signal transduction by measuring luminol-enhanced chemiluminescence (CL). The response patterns fell into three categories. The mAb Mo4, OKM3, OKM6 and antibodies specific for Fc receptor (FcR) type I and II did not mediate signal transduction directly or were weak triggers, but upon second-order cross-linking by goat anti-mouse immunoglobulin (Ig) F(ab')2 or rabbit anti-mouse Ig, a strong CL response was induced. The mAb recognizing different epitopes on CD11b (complement receptor type III alpha chain) were unique in their ability to induce a CL response either by direct stimulation or by second-order cross-linking. The mAb 3G8 recognizing FcR type III (FcRIII; CD16) on a monocyte subpopulation and CD36-specific monoclonals directly elicited a CL response. A response of similar magnitude was obtained with 3G8 F(ab')2 or with intact 3G8. Furthermore, elutriation-centrifugation-purified monocytes were stimulated by 3G8 to a similar extent as unseparated mononuclear cells, whereas lymphocytes did not respond. This suggests that a FcRIII-expressing monocyte subpopulation may mediate effector functions, including the generation of reactive oxygen species, via FcRIII triggering. Our finding that anti-CD36 F(ab')2 directly induces an oxidative burst is the first evidence that CD36 itself is a trigger molecule. CD36, which is thought to interact with erythrocytes infected with Plasmodium falciparum and with thrombospondin, may constitute a signal-transducing element and thus may have functions extending beyond mediation of adherence. The present CL system constitutes a simple assay for detecting mAb directed against monocyte surface signalling elements. Probing mAb for signal transduction requires suspended cells and antibodies in the fluid phase in order to avoid inadvertent FcR triggering, which may occur when cells are stimulated by surface-adherent whole antibodies.

Trezzini, C; Jungi, T W; Spycher, M O; Maly, F E; Rao, P

1990-01-01

411

The Oxidant-Scavenging Abilities in the Oral Cavity May Be Regulated by a Collaboration among Antioxidants in Saliva, Microorganisms, Blood Cells and Polyphenols: A Chemiluminescence-Based Study  

PubMed Central

Saliva has become a central research issue in oral physiology and pathology. Over the evolution, the oral cavity has evolved the antioxidants uric acid, ascorbate reduced glutathione, plasma-derived albumin and antioxidants polyphenols from nutrients that are delivered to the oral cavity. However, blood cells extravasated from injured capillaries in gingival pathologies, or following tooth brushing and use of tooth picks, may attenuate the toxic activities of H2O2 generated by oral streptococci and by oxidants generated by activated phagocytes. Employing a highly sensitive luminol-dependent chemiluminescence, the DPPH radical and XTT assays to quantify oxidant-scavenging abilities (OSA), we show that saliva can strongly decompose both oxygen and nitrogen species. However, lipophilic antioxidant polyphenols in plants, which are poorly soluble in water and therefore not fully available as effective antioxidants, can nevertheless be solubilized either by small amounts of ethanol, whole saliva or also by salivary albumin and mucin. Plant-derived polyphenols can also act in collaboration with whole saliva, human red blood cells, platelets, and also with catalase-positive microorganisms to decompose reactive oxygen species (ROS). Furthermore, polyphenols from nutrient can avidly adhere to mucosal surfaces, are retained there for long periods and may function as a “slow- release devises” capable of affecting the redox status in the oral cavity. The OSA of saliva is due to the sum result of low molecular weight antioxidants, albumin, polyphenols from nutrients, blood elements and microbial antioxidants. Taken together, saliva and its antioxidants are considered regulators of the redox status in the oral cavity under physiological and pathological conditions.

Ginsburg, Isaac; Kohen, Ron; Shalish, Miri; Varon, David; Shai, Ella; Koren, Erez

2013-01-01

412

Development of a multichannel Fourier-transform spectrometer to measure weak chemiluminescence: Application to the emission of singlet-oxygen dimol in the decomposition of hydrogen peroxide with gallic acid and K 3[Fe(CN) 6  

NASA Astrophysics Data System (ADS)

A Fourier-transform spectrometer equipped with a Savart-plate polarization interferometer was developed for observation of weak chemiluminescence and applied to a measurement of emission spectra in the decomposition of hydrogen peroxide with gallic acid and K 3[Fe(CN) 6]. The band appearing at ˜580 nm in the chemiluminescence spectrum was assigned to the emission of singlet-oxygen dimol, the peak wavelength being shifted from that observed in the reaction of hydrogen peroxide with sodium hypochlorite, ˜633 nm. The band intensity was increased with the increasing concentration of K 3[Fe(CN) 6] up to ˜100 mM, and thereafter the peak wavelength was shifted from 580 to 700 nm with a decrease in the intensity.

Tsukino, Kazuo; Satoh, Toshihiro; Ishii, Hiroshi; Nakata, Munetaka

2008-05-01

413

Biochemical characterization of lucigenin (Bis-N-methylacridinium) as a chemiluminescent probe for detecting intramitochondrial superoxide anion radical production.  

PubMed

Direct detection of intramitochondrial superoxide anion radical (O(-*)(2)) production is of critical importance for investigating the pathophysiological consequences resulting from altered cellular reactive oxygen homeostasis. The purpose of this study with isolated mitochondria was to characterize the biochemical basis for lucigenin as a chemiluminescent probe to detect intramitochondrial O(-*)(2) production. Incubation of isolated mitochondria with lucigenin at non-redox cycling concentration produced lucigenin-derived chemiluminescence (LDCL), which was increased markedly by mitochondrial substrates, pyruvate/malate or succinate. The LDCL was reduced greatly by the membrane permeable superoxide dismutase (SOD) mimetics, 2,2,6,6-tetramethylpiperidine-N-oxyl and Mn(III)tetrakis(1-methyl-4-pyridyl)porphyrin, but not by Cu,Zn-SOD. With an ion-pair HPLC method, a concentration-dependent accumulation of lucigenin was detected within mitochondria. The accumulation of lucigenin by mitochondria was reduced markedly in the presence of carbonyl cyanide p-(trifluoromethoxy)phenyhyldrazone, an uncoupler known to dissipate the mitochondrial membrane potential. With submitochondrial particles, we observed that both complexes I and III of the mitochondrial electron transport chain appear to be able to catalyze the one electron reduction of lucigenin, a critical step involved in LDCL. After incubation of mitochondria with lucigenin at non-redox cycling concentrations, formation of N-methylacridone, the proposed end product of the reaction pathway leading to LDCL, within the mitochondrial fraction was also detected. In addition, a significant linear correlation was observed between the LDCL and either the lucigenin accumulation or the N-methylacridone formation within the mitochondria. Taken together, our results conclusively demonstrate that when properly used LDCL can reliably detect intramitochondrial O(-*)(2) production. PMID:10448072

Li, Y; Stansbury, K H; Zhu, H; Trush, M A

1999-08-19

414

Synthesis and properties of chemiluminescent acridinium ester labels with fluorous tags.  

PubMed

Acridinium dimethylphenyl esters are highly sensitive chemiluminescent labels that are used in clinical diagnostics. Light emission from these labels is triggered with alkaline peroxide in the presence of the cationic surfactant cetyltrimethylammonium chloride (CTAC). CTAC compresses emission times of these labels to <5 seconds and also increases overall light yield 3-4 fold. The observed enhancement in acridinium ester chemiluminescence (light yield) is quite sensitive to the polarity of the micellar interface. In the current study, we report the synthesis of new acridinium ester labels with fluorous tags of varying fluorine content and their chemiluminescence in the presence of cationic micelles of CTAC, anionic micelles of sodium perfluorooctanoate (SPFO) as well as mixed micelles of CTAC and SPFO. These studies indicate that in the presence of the mixed micelle system of CTAC and SPFO and at low mole fractions of SPFO, polarity of the mixed micelle interface is lower than that of CTAC leading to a greater enhancement of chemiluminescence for both fluorinated acridinium esters as well as a structurally analogous but non-fluorinated acridinium ester. Chemiluminescence stability of the fluorinated acridinium esters was either comparable to or better than the stability of the non-fluorinated acridinium ester. Non-specific binding to paramagnetic microparticles was higher for fluorinated acridinium esters requiring a surfactant wash to reduce their non-specific binding to the same extent as that observed for the non-fluorinated acridinium ester. PMID:24788381

Natrajan, Anand; Wen, David; Sharpe, David

2014-05-21

415

A study on the system of nonaqueous microchip electrophoresis with on-line peroxyoxalate chemiluminescence detection.  

PubMed

This article describes a further development of our previously reported miniaturized analysis system of microchip electrophoresis with on-line chemiluminescence detection. The system, developed first time for nonaqueous microchip electrophoresis with peroxyoxalate chemiluminescence detection, consists of a suction pressure device for sample or reagent introduction, a constant voltage supplied for electrophoretic separation, an either hydrophilic or hydrophobic porous polymer plug for preventing chemiluminescence reagent flowing upstream and a spiral detection channel for enhancement of both detection sensitivity and reproducibility. Especially, by using organic solvent as BGE medium, the developed system avoided the interface problem between aqueous running buffer and low-water-content chemiluminescence solvent in previous reports. The influencing factors on chemiluminescence signal were optimized using rhodamine 6G as model molecule. The system performance was further investigated in the experiment of separation of hydrophilic rhodamine dyes and analysis of hydrophobic polycyclic aromatic hydrocarbon, providing the detection limit (S/N = 3) of 3.5 nmol/L for rhodamine 123, 6.8 nmol/L for rhodamine 6G, and 60 nmol/L for 1-aminopyrene, respectively. The experimental results showed the system offered a number of benefits, including compact structure, high sensitivity, good reproducibility, and a wide range of application prospect. PMID:23339027

Hu, Hong-Mei; Yin, Xue-Feng; Wang, Xiu-Zhong; Shen, Hong

2013-02-01

416

Sensitive determination of triacetone triperoxide explosives using electrogenerated chemiluminescence.  

PubMed

Sensitive and selective detection and quantification of high explosive triacetone triperoxide (TATP) with electrogenerated chemiluminescence (ECL) at a glassy carbon electrode in water-acetonitrile solvent mixture were reported. In the presence of ruthenium(II) tris(bipyridine), TATP or hydrogen peroxide derived from TATP via UV irradiation or acid treatment produced ECL emissions upon cathodic potential scanning. Interference from hydrogen peroxide on TATP detection was eliminated by pretreatment of the analyte with catalase enzyme. Selective detection of TATP from hexamethylene triperoxide diamine (HMTD, another common peroxide-based explosive) was realized by comparing ECL responses obtained from the anodic and the cathodic potential scanning; TATP produced ECL upon cathodic potential scanning only, whereas HMTD produced ECL upon both cathodic and anodic potential scanning. The hydroxyl radical formed after the electrochemical reduction of TATP was believed to be the key intermediate for ECL production, and its stability was strongly dependent on the solution composition, which was verified with electron paramagnetic resonance spectroscopy. A detection limit of 2.5 ?M TATP was obtained from direct electrochemical reduction of the explosive or hydrogen peroxide derived from TATP in 70/30% (v/v) water-acetonitrile solutions, which was ~400 times lower than that reported previously based on liquid chromatography separation and Fourier transform infrared detection. PMID:23885721

Parajuli, Suman; Miao, Wujian

2013-08-20

417

Chemiluminescence. 1977-April 1980, 1980 (citations from the International Aerospace Abstracts data base). Report for 1977-April 1980  

SciTech Connect

Cited works describe the use of chemiluminescence for aurora and upper-atmosphere analyses, evaluation of chemical laser candidates, studies of jet and combustor dynamics, smog analysis and other work. Sources and forms of chemiluminescence and measurement techniques are described. (Contains 92 citations)

Young, C.G.

1980-05-01

418

Label free and homogeneous histone sensing based on chemiluminescence resonance energy transfer between lucigenin and gold nanoparticles.  

PubMed

In this paper, a label free and homogeneous protocol without recognition elements such as antibody or DNA based on nonenzymatic chemiluminescence resonance energy transfer between lucigenin and gold nanoparticles (AuNPs) is developed for the detection of histone. This chemiluminescence resonance energy transfer process originated from a chemiluminescent donor-acceptor pair in which the chemiluminescence system of the lucigenin-H2O2 as a donor and AuNPs as an acceptor owing to the overlapping of the chemiluminescence spectrum of the lucigenin-H2O2 system and the absorption spectrum of AuNPs, leading to a significant decrease in chemiluminescence signal from the lucigenin-H2O2 system. However, the presence of histone resulted in the aggregation of AuNPs via the electrostatic interaction between negatively charged AuNPs and positively charged histone, which inhibited the chemiluminescence resonance energy transfer process. Thus the chemiluminescence signal of the lucigenin-H2O2 system was restored. This could be used for the detection of histone with a linear range of 30-500ng/mL, and a detection limit of 25ng/mL. This sensitivity increased about 4 orders of magnitude over that of the reported fluorometric method. The proposed strategy for the detection of histone is simple, facile, reliable, and opens a new avenue for the determination of histone. PMID:23603126

He, Yi; Cui, Hua

2013-09-15

419

Molecular connectivity as a relevant new tool for predicting analytical behavior: A survey of chemiluminescence and chromatography  

Microsoft Academic Search

We present a critical presentation and discussion on molecular connectivity applied to analytical fields, giving special attention to predicting the chemiluminescent behavior of pharmaceuticals and pesticides. Molecular connectivity has been largely applied to predict the therapeutic effects of pharmaceuticals and rarely to predictions in analytical chemistry – basically to chromatographic processes and recently to liquid-phase chemiluminescence.

M. J. Duart; L LAHUERTAZAMORA; G ANTONFOS; J MARTINEZCALATAYUD

2005-01-01

420

Chemiluminescent and colorigenic detection of cherry leaf roll virus with digoxigenin-labeled RNA probes.  

PubMed

Digoxigenin-labeled RNA probes were used to detect cherry leaf roll virus in infected plants. A dot-blot hybridization immunoenzymatic assay in both crude sap extracts and partially purified tissue with a colorigenic and chemiluminescent detection was developed. The use of the new AMPPD substrate was found to be effective in clarified sap extracts in conditions were the colorigenic detection method failed. Both detection assays were effective when using unfractionated nucleic acid preparations, the chemiluminescent being five times more sensitive than the colorigenic. The chemiluminescent hybridization assay makes it possible to detect the virus at the picogram level. The non-radioactive dot-blot hybridization techniques described here turned out to be very suitable for plant virus diagnosis. The sensitivity of this method and those obtained by ELISA or radioactive dot-blot described previously is compared. PMID:7505786

Más, P; Sánchez-Navarro, J A; Sánchez-Pina, M A; Pallás, V

1993-11-01

421

A competitive chemiluminescence enzyme immunoassay for rapid and sensitive determination of enrofloxacin  

NASA Astrophysics Data System (ADS)

With alkaline phosphatase (ALP)-adamantane (AMPPD) system as the chemiluminescence (CL) detection system, a highly sensitive, specific and simple competitive chemiluminescence enzyme immunoassay (CLEIA) was developed for the measurement of enrofloxacin (ENR). The physicochemical parameters, such as the chemiluminescent assay mediums, the dilution buffer of ENR-McAb, the volume of dilution buffer, the monoclonal antibody concentration, the incubation time, and other relevant variables of the immunoassay have been optimized. Under the optimal conditions, the detection linear range of 350-1000 pg/mL and the detection limit of 0.24 ng/mL were provided by the proposed method. The relative standard deviations were less than 15% for both intra and inter-assay precision. This method has been successfully applied to determine ENR in spiked samples with the recovery of 103%-96%. It showed that CLEIA was a good potential method in the analysis of residues of veterinary drugs after treatment of related diseases.

Yu, Fei; Wu, Yongjun; Yu, Songcheng; Zhang, Huili; Zhang, Hongquan; Qu, Lingbo; Harrington, Peter de B.

422

[Chemiluminescence of blood and urine in children with pyelonephritis and glomerulonephritis].  

PubMed

It was established during the study of blood serum and urine chemiluminescence in 57 children with pyelonephritis and in 38 children with glomerulonephritis that in the active disease stage, the intensity of overfaint luminescence rises as a result of lipid peroxidation (LPO) activation, accumulation of lipid hydroperoxides and oxygen-containing radicals. Four types of the kinetic curves of urine chemiluminescence were identified. They characterize the correlation between LPO activation and the level of antiradical defence in patients suffering from pyelo- and glomerulonephritis. The measurement of urine chemiluminescence in patients afflicted with pyelonephritis and glomerulonephritis may be one of the criteria for administering drugs possessing antiradical and antioxidant activity and can also be used in the control over the treatment efficacy. PMID:2628909

Ma?dannik, V G; Afonina, G B; Bordonos, V G; Bagdasarova, I V

1989-01-01

423

Utilization of the human cell line HL60 for chemiluminescence based detection of microorganisms and related substances  

Microsoft Academic Search

In this paper we describe a new pyrogen assay using the human leukemia cell line HL-60. The cell line is differentiated using all-trans retinoic acid (ATRA) to generate a cell population that resembles mature granulocytes. The differentiated HL-60 cell is capable of generating reactive oxygen species (ROS) when challenged with pyrogenic substances. In a luminol enhanced chemilumimetric assay the responsiveness

Michael Timm; Erik W. Hansen; Lise Moesby; Jens D. Christensen