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1

Applications of the luminol chemiluminescent reaction in analytical chemistry  

Microsoft Academic Search

This critical review discusses the results published between 2000 and 2005 on the development of analytical systems based\\u000a on the luminol chemiluminescent and electrochemiluminescent reactions. An increasing number of non-specific detection systems\\u000a based on the enhancing, inhibiting or catalysing effect of a large range of compounds have been published. Possible detected\\u000a compounds and their concomitant presence in samples are discussed.

Christophe A. Marquette; Loïc J. Blum

2006-01-01

2

Chemiluminescence flow injection analysis of reducing agents based on the luminol reaction  

Microsoft Academic Search

The chemiluminescence reaction of luminol with reducing agents was examined by a flow injection method. It was found that Chemiluminescence was directly produced by the reaction of reducing agents with luminol in the presence of a catalyst in an alkaline solution. Hexacyanoferrate(III) and hexacyanoferrate(II) were used as catalyst for the reaction. Detection limits (S\\/N=3) of uric acid, phenacyl alcohol, cortisone,

Hiroaki Kubo; Akira Toriba

1997-01-01

3

Luminol chemiluminescence HPLC reaction detector for amino acids and other ligands  

Microsoft Academic Search

A detector for HPLC is based on suppression of chemiluminescence from the Cu(II)-luminol-peroxide reaction. Analytes which complex Cu(II) reduce the free Cu(II) concentration and thus the observed chemiluminescence intensity. The degree of chemiluminescence suppression is a measure of the analyte concentrations. Detection limits are in the range of 1 pmole-1 nmole for amino acids (both primary and secondary without derivatization),

P. J. Koerner; Timothy A. Nieman

1987-01-01

4

Catalysis by manganese (III) 8-hydroxyquinolinates of the chemiluminescent reaction of luminol with hydrogen peroxide  

SciTech Connect

This paper examines the kinetics of the reaction of luminol with H/sub 2/O/sub 2/ in the presence of Mn (III) 8-hydroxyquinolinate according to the data of measurements of the chemiluminescence intensity and the yield of light in this reaction. A reaction mechanism was proposed, providing for the oxidation of luminol by complexes of Mn (IV) that are formed in the decoposition of H/sub 2/O/sub 2/.

Kalinichenko, I.E.; Matveeva, E.Y.; Pilipenko, A.T.

1985-09-01

5

Correlation between hemin content and the chemiluminescent luminol reaction with bacteria.  

PubMed Central

Protohemin and covalently bound hemin were determined in eight aerobic bacterial strains. A good correlation between protohemin content and luminol reactivity was found. The ratio of luminol reaction to protohemin for the eight investigated strains was essentially identical to that of pure protohemin, 0.7 X 10(16) mV/mol. Covalently bound hemin contributed to the chemiluminescence to a minor extent only (0.7 X 10(14) mV/mol, in accordance with earlier observations of the lower reactivity of cytochrome c and related compounds. A difference in reaction kinetics of the luminol reaction with covalently bound hemin (slower reaction than protohemin) and protohemin was observed in vivo as well as in vitro. The phenomenon could be used to differentiate between strains with different hemin composition.

Ewetz, L; Thore, A

1978-01-01

6

A luminol chemiluminescence method for sensing histidine and lysine using enzyme reactions.  

PubMed

The analysis of free amino acids in urine and plasma is useful for estimating disease status in clinical diagnoses. Changes in the concentration of free amino acids in foods are also useful markers of freshness, nutrition, and taste. In this study, the specific interaction between aminoacyl-tRNA synthetase (aaRS) and its corresponding amino acid was used to measure amino acid concentrations. Pyrophosphate released by the amino acid-aaRS binding reaction was detected by luminol chemiluminescence; the method provided selective quantitation of 1.0-30?M histidine and 1.0-60?M lysine. PMID:23973428

Kugimiya, Akimitsu; Fukada, Rie; Funamoto, Daiki

2013-08-22

7

Influence of the luminol chemiluminescence reaction on the confirmatory tests for the detection and characterization of bloodstains in forensic analysis  

Microsoft Academic Search

Preliminary tests for the detection of stains at crime scenes aim to focus the police work making them more efficient in the combat of criminality. The application of the luminol chemiluminescence reaction (3-aminoftalhidrazida) in presumptive tests for the detection of bloodstains is known for more than 40 years in forensic science. This reaction is based on the emission of light

V. R. D. Santos; W. X. Paula; E. Kalapothakis

2009-01-01

8

Luminol Chemiluminescence in Urine Analysis  

Microsoft Academic Search

The purpose of the present review is to sketch out the scope of luminol chemiluminescence in human urine analysis. Practical considerations and experimental requirements are indicated. The literature revised covers the papers of analytical interest that have appeared in approximately the last six years.

Ana María Jiménez Moreno; María José Navas Sánchez

2006-01-01

9

Comparison of the Absolute Quantum Yields of the Gas Phase O/NO Reaction and the Liquid Phase Luminol Oxidation Chemiluminescence Light Standards.  

National Technical Information Service (NTIS)

Chemiluminescence intensities from the O/NO gas phase reaction and from luminol (3-aminophthal hydrazide) oxidation in the liquid phase have been measured under the same conditions of geometry, optics and detection over the wavelength range 440-530 nm. Th...

A. Fortijn J. Lee

1971-01-01

10

Passive micromixer for luminol-peroxide chemiluminescence detection.  

PubMed

This paper reports a microchip with an integrated passive micromixer based on chaotic advection. The micromixer with staggered herringbone structures was used for luminol-peroxide chemiluminescence detection. The micromixer was examined to assess its suitability for chemiluminescence reaction. The relationship between the flow rate and the location of maximum chemiluminescence intensity was investigated. The light intensity was detected using an optical fiber attached along the mixing channel and a photon detector. A linear correlation between chemiluminescence intensity and the concentration of cobalt(ii) ions or hydrogen peroxide was observed. This microchip has a potential application in environmental monitoring for industries involved in heavy metals and in medical diagnostics. PMID:21552614

Lok, Khoi Seng; Kwok, Yien Chian; Nguyen, Nam-Trung

2011-05-09

11

Chemiluminescence of Luminol in Aqueous Solutions, a Pulse Radiolytic Study.  

National Technical Information Service (NTIS)

The mechanism of chemiluminescence was studied when the luminol radical and superoxide were generated simultaneously. Hydroperoxide was formed at the first reaction step with a pKa value between 11 and 12. The dissociated form was found to undergo a monom...

J. Lind

1980-01-01

12

Determination of phenol by flow-injection with chemiluminescence detection based on the hemin-catalysed luminol-hydrogen peroxide reaction  

NASA Astrophysics Data System (ADS)

This study established a novel flow injection (FI) methodology for the determination of phenol in aqueous samples based on luminol chemiluminescence (CL) detection. The method was based on the inhibition that phenol caused on the hemin-catalysed chemiluminescence reaction between luminol and hydrogen peroxide in alkaline solution. Optimum conditions and possible mechanisms have been investigated. The linear range was 2.0 × 10 -9 to 4.0 × 10 -7 g mL -1 for phenol. The proposed method is sensitive with a detection limit of 4.0 × 10 -10 g mL -1. The relative standard deviation for 11 measurements was 2.3% for 1.0 × 10 -7g mL -1 phenol. The method was applied for the determination of phenol in waste water samples. The results obtained compared well with those by an official method.

Liu, Wenwen; Cao, Wei; Liu, Weihua; Du, Kang; Gong, Pixue

2012-01-01

13

Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere  

Microsoft Academic Search

An instrument has been designed and constructed for the simultaneous determination of nitrogen dioxide (NO2) and peroxyacetyl nitrate (PAN) in atmospheric samples. The instrument's design is based on separation by fast gas chromatography (GC) with a 30 ft capillary column (DB-1) followed by detection by luminol chemiluminescence. The chemiluminescent reaction between NO2 or PAN and luminol takes place at the

Nancy A. Marley; Jeffrey S. Gaffney; Robert V. White; Luis Rodriguez-Cuadra; Scott E. Herndon; Ed Dunlea; Rainer M. Volkamer; Luisa T. Molina; Mario J. Molina

2004-01-01

14

Ultrasensitive assay of azithromycin in medicine and bio-Fluids based on its enhanced luminol–H 2O 2 chemiluminescence reaction using flow injection technique  

Microsoft Academic Search

A simple flow injection chemiluminescence method with synergistic enhancement has been investigated for the rapid and sensitive determination of azithromycin. The synergistic action was significant in the chemiluminescence system of luminol–hydrogen peroxide with azithromycin as an enhancer. The enhanced chemiluminescence intensity was linear with the concentration of azithromycin over the range from 0.1 pg mL?1 to 1.0 ng mL?1 (r2=0.9988)

Zhenghua Song; Changna Wang

2003-01-01

15

Human bronchoalveolar lavage cells and luminol-dependent chemiluminescence  

Microsoft Academic Search

Human bronchoalveolar lavage cells from several different disease states were examined by the technique of zymosan-stimulated, luminol-dependent chemiluminescence. Light production correlated well with polymorphonuclear leucocyte contamination of the alveolar macrophage suspension but not with lymphocyte contamination. Regression analysis indicated that human alveolar macrophages produce little if any luminol-dependent chemiluminescence. Further investigation of metabolic activity, using measurements of superoxide release, oxygen

A J Williams; P J Cole

1981-01-01

16

Mechanism of the Ferricyanide-Catalyzed Chemiluminescence of Luminol.  

National Technical Information Service (NTIS)

A study of the mechanism of the potassium ferricyanide chemiluminescent oxidation of luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) is reported. An important feature of the proposed mechanism is the one-electron oxidation of the luminol dianion by fer...

P. B. Shevlin H. A. Neufeld

1968-01-01

17

Effect of gold nanoparticle as a novel nanocatalyst on luminol–hydrazine chemiluminescence system and its analytical application  

Microsoft Academic Search

In this work the catalytic role of unsupported gold nanoparticles on the luminol–hydrazine reaction is investigated. Gold nanoparticles catalyze the reaction of hydrazine and dissolved oxygen to generate hydrogen peroxide and also catalyze the oxidation of luminol by the produced hydrogen peroxide. The result is an intense chemiluminescence (CL) due to the excited 3-aminophthalate anion. In the absence of gold

A. Safavi; G. Absalan; F. Bamdad

2008-01-01

18

Simple and Sensitive Assay for Nucleic Acids Using their Quenching Effect on the Chemiluminescence Reaction Between Luminol and Hydrogen Peroxide with Manganese-Tetrasulfonatophthalocyanine as a New Catalyst  

Microsoft Academic Search

The manganese-tetrasulfonatophthalo-cyanine (MnTSPc) catalyzed luminol-hydrogen peroxide chemiluminescence (CL) system can be quenched in the presence of nucleic acids. A new and highly sensitive CL quenching method for determining nucleic acids in aqueous solutions has been developed. Under optimum conditions, linear relationships were found between the quenched intensity of CL and the concentration of nucleic acids in the range 0.10–2.0?µg?mL -1

Yongxin Li; Changqin Zhu; Lun Wang; Donghui Li; Jingou Xu

2003-01-01

19

Ferrous iron-induced luminol chemiluminescence: a method for hydroxyl radical study  

Microsoft Academic Search

We have investigated the chemiluminescence signal of the ferrous iron in the presence of the luminol and lucigenin. Ferrous, but not ferric, iron produced a transient signal in the presence of luminol, but not lucigenin. Ferrous iron-induced luminol chemiluminescence was significantly inhibited in a concentration-dependent manner by superoxide dismutase (SOD) and catalase. Specific hydroxyl radical scavengers, mannitol and dimethyl sulfoxide

Gülüzar Y?ld?z; A. Tuncay Demiryürek

1998-01-01

20

Synthesis and Characterization of Luminol Persulphate Chemiluminescence in Aqueous Amines  

NASA Astrophysics Data System (ADS)

The chemiluminescence (CL) emission spectra of luminol were recorded using Fuss spectrograph in different aqueous aliphatic amines using sodium persulphate alone and mixture with hydrogen peroxide as an oxidant. The CL emission spectra after resolution showed two emission bands at 425 and 455 nm. The CL mechanism was explained on the basis of two exited state species formed during oxidation of luminol. The CL of luminol is found to be very weak as persulphate slowly produced oxygen. The glow become intense with time as more and more oxygen is made available for oxidation of luminol. The mixture of hydrogen peroxide and sodium persulphate is found to be more effective in producing intense and long lived CL glow for luminol. The CL emission band of luminol by using sodium persulphate and mixture with hydrogen peroxide is explained on the basis of formation of exited singlet and triplet state of 3-aminophthalate ion (3-APA). The shorter wavelength emission band of 425 nm is found to be very weak in intensity as compared to longer wavelength emission band of 455 nm. Thus phosphoresce is favored in case of persulphate CL of luminol.

Raut, V. M.; More, P. S.; Khollam, Y. B.; Sonone, R. S.; Kondawar, S. B.; Koinkar, Pankaj

21

Studies on the Chemiluminescence of Luminol in Dimethylsulfoxide and Dimethysulfoxide-Water Mixtures.  

National Technical Information Service (NTIS)

The absorption, fluorescence, and chemiluminescence (CL) characteristics of luminol have been studied in basic dimethylsulfoxide (DMSO) and various basic DMSO-water mixed solvents. It has been shown that the luminol dianion can be produced quantitatively ...

D. M. Hercules J. D. Gorsuch

1971-01-01

22

Optimization of peroxynitrite-luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide.  

PubMed

We established a peroxynitrite-luminol chemiluminescence system for detecting peroxynitrite in cell culture solution exposed to carbon disulphide (CS(2)). Three factors, including exposure time to ozone (Factor A), volume of peroxynitrite (ONOO(-)) solution (Factor B) and luminol concentrations (Factor C) at three levels were selected and the combinations were in accordance with orthogonal design L(9) (3(4)). Peroxynitrite was generated from the reaction of ozone and 0.01 mol/L sodium azide (NaN(3)) dissolved in carbonic acid buffer solution (pH 11), and it was reacted with luminol to yield chemiluminescence. The peak value, peak time and kinetic curve of the light emission were observed. The selected combination conditions were 50 s ozone, 800 micro L peroxynitrite and 0.001 mol/L luminol solution. Cell culture solution with CS(2) enhanced the emission intensity of chemiluminescence (F = 8.38, p = 0.018) and shortened the peak time to chemiluminescence (F = 139.00, p = 0.0001). The data demonstrated that this luminol chemiluminescence system is suitable for detecting peroxynitrite in cell culture solutions for evaluating the effect of CS(2) on endothelial cells. PMID:14587075

Chen, Shao L; Jian, Le; Lang, Hui Q

23

Development of a novel chemiluminescence method for the determination of cefazolin sodium in injectable powder and human urine based on a luminol-Cu(III) complex reaction in alkaline medium.  

PubMed

A novel chemiluminescence (CL) method was developed for the determination of cefazolin sodium based on the CL reaction between the [Cu(HIO6)2](5-) Cu(III) complex and luminol in alkaline solution. Results showed that CL emission of Cu(III) complex-luminol in alkaline medium was significantly different from that in acidic medium. A possible mechanism of the enhanced effect of cefazolin on CL emission of the [Cu(HIO6)2](5-)-luminol system was proposed. The effect of the reaction conditions on CL emissions was examined. Under optimized conditions, a good linear relationship was obtained between CL intensity and concentrations of cefazolin sodium in the range of 2.0 x 10(-8) to 2.0 x 10(-6) g/mL with a correlation coefficient of R(2) = 0.9978. The limit of detection was 4.58 x 10(-9) g/mL. The proposed method was applied for the determination of cefazolin sodium in real samples with recoveries of 82.0-109% with an RSD of 0.7-2.1%. The proposed method was successfully used for the determination of cefazolin sodium in injectable powder preparations and human urine with satisfactory results. PMID:22807137

Sun, Hanwen; Wang, Juan; Wang, Ting

2012-07-15

24

The effect of electrode material on the electrogenerated chemiluminescence of luminol  

SciTech Connect

This paper reports on the oxidation of luminol and its concomitant electrogenerated chemiluminescence (ECL) which were studied at several electrode materials by voltammetry and chronoamperometry. The ECL intensity (I{sub ECL}) was inversely related to the activity of the electrodes. The lowest I{sub ECL}) was measured when luminol was oxidized to 3-aminophthalate (n {approx equal}4 eq mol{sup {minus}1}) at a nearly mass-transport limited rate at glassy carbon. The ECL kinetics were studied and the order of the reaction with respect to luminol was 3/2 at concentrations to ca. 1 mM when O{sub 2} was the coreactant. In the presence of H{sub 2}O{sub 2}, the ECL reaction was first order with respect to luminol. A reaction mechanism is proposed that is consistent with the inetic data and the inverse relationship between electrode activity and I{sub ECL}. The implications of these results are discussed with respect to imaging the spatial distribution of current density at electrode surfaces, including that of PbO{sub 2} films activated by adsorbed Bi(V). A value of 6.6 {times} 10{sup {minus}6} cm{sup 2} s{sup {minus}1} was determined for the diffusion coefficient of luminol in 0.1M NaOH.

Vitt, J.E.; Johnson, D.C. (Ames Lab., IA (United States)); Engstrom, R.C. (Dept. of Chemistry, Univ. of South Dakota, Vermillion, SD (US))

1991-06-01

25

Study on the chemiluminescence behavior of bovine serum albumin with luminol and its analytical application  

NASA Astrophysics Data System (ADS)

In this paper, the luminescence behavior of bovine serum albumin (BSA) and luminol was first studied by flow injection chemiluminescence (CL). It was found that the hyperchromic effect of luminol in the presence of BSA led to the acceleration of the electrons transferring rate of excited 3-aminophthalate, which greatly enhanced the CL intensity of luminol/dissolved oxygen reaction. The increments of CL intensity were proportional to the concentrations of BSA with a linear range from 0.01 to 7 nmol L -1. It was also found that azithromycin could inhibit the CL intensity of luminol/BSA reaction. The decrements of CL intensity were logarithm over the concentrations of azithromycin ranging from 0.1 to 700 ng mL -1. At a flow rate of 2.0 mL min -1, a complete analytical process, which included sampling and washing, could be performed within 30 s with relative standard deviations of less than 3.1%. This proposed method was successfully applied in assaying azithromycin in pharmaceutical and human serum samples with recoveries from 91.0 to 104.3%. The possible luminescence mechanism of luminol/BSA/azithromycin reaction was discussed in detail by CL, UV and fluorescence methods.

Tan, Xijuan; Song, Zhenghua; Chen, Donghua; Wang, Zhuming

2011-06-01

26

Properties of Kinetics of Chemiluminescence of Luminol Excited by a Nitrogen Laser  

NASA Astrophysics Data System (ADS)

The kinetics of chemiluminescence of luminol C8H7N3O2 excited by a pulsed nitrogen laser is studied The kinetics of chemiluminescence of free luminol molecules is compared with that of luminol molecules in the complex UO2(C8H6N3O2)2. Photochemical consequences of excitation of the UO2(C8H6N3O2)2 are considered.

Gorshkov, N. G.; Izosimov, I. N.; Kazimov, A. A.; Kolychev, S. V.; Kudryashev, N. A.; Mashirov, L. G.

2002-02-01

27

Flow-injection chemiluminescence determination of dihydralazine sulfate in serum using luminol and diperiodatocuprate (III) system  

NASA Astrophysics Data System (ADS)

A novel flow-injection chemiluminescence (CL) method for the determination of dihydralazine sulfate (DHZS) is described. The method is based on the reaction of luminol and diperiodatocuprate (K 2[Cu(H 2IO 6)(OH) 2], DPC) in alkaline medium to emit CL, which is greatly enhanced by DHZS. The possible CL mechanism was first proposed based on the kinetic characteristic, CL spectrum and UV spectra. The optimum condition for the CL reaction was in detail studied using flow-injection system. The experiments indicated that under optimum condition, the CL intensity was linearly related to the concentration of DHZS in the range of 7.0 × 10 -9 to 8.6 × 10 -7 g mL -1 with a detection limit (3 ?) of 2.1 × 10 -9 g mL -1. The proposed method had good reproducibility with the relative standard deviation 3.1% ( n = 7) for 5.2 × 10 -8 g mL -1 of DHZS. This method has the advantages of simple operation, fast response and high sensitivity. The special advantage of the system is that very low concentration of luminol can react with DPC catalyzed by DHZS to get excellent experiment results. And CL cannot be observed nearly when luminol with same concentration reacts with other oxidants, so luminol-DPC system has higher selectivity than other luminol CL systems. The method has been successfully applied to determine DHZS in serum.

Yang, Chunyan; Zhang, Zhujun; Wang, Jinli

2010-01-01

28

Luminol-type chemiluminescence derivatization reagents for liquid chromatography and capillary electrophoresis  

Microsoft Academic Search

The present paper provides the principles for chemiluminescence of luminol-type compounds and their wide and powerful application to the detection system in liquid chromatography and capillary electrophoresis as derivatization reagents. The reagents can be classified into two types, chemiluminescence labeling and chemiluminogenic reagents. The former reagents are highly chemiluminescent themselves and used for tagging their intense chemiluminophores to analytes, whereas

Masatoshi Yamaguchi; Hideyuki Yoshida; Hitoshi Nohta

2002-01-01

29

Magnetic modulation of the chemiluminescence intensity in the oxidation of luminol by potassium ferricyanide  

SciTech Connect

This paper attempts the experimental detection and investigation of the magnetic field-dependent radical steps in the oxidation of luminol by potassium ferricyanide. It was found that it is in fact possible to affect the chemiluminescence yield by the use of a low intensity magnetic field (ca 100 Oe) and to relate the observed effect to a hyperfine interaction in the radical pairs formed during the reaction. Solutions of LH/sub 2/ and K/sub 3/Fe (CN)/sub 6/ in alkaline aqueous solution (0.1 M NaOH) were delivered continuously through a mixer into an optical cuvette. A block diagram of the equipment is shown. The chemiluminescent light was directed through a light guide to an FEU-79 photoamplifier, protected by a special shield from the action of scattered magnetic fields. The derivative of the magnetic effect was examined and it was established that there is no deviation from saturation of the magnetic effect up to 3.5 kOe. The results demonstrate that in the stages preceding the formation of light emitter an interaction occurs between two paramagnetic particles. It is also shown that it is in principle possible to record the ESR spectrum of these luminol radicals with respect to the chemiluminescence, using the reaction-yield-detected magnetic resonance method.

Tribel', M.M.; Frankevich, E.L.; Leksin, A.N.; Morozov, A.K.

1986-04-01

30

Inhibition of superoxide dismutase, Vitamin C and glutathione on chemiluminescence produced by luminol and the mixture of sulfite and bisulfite  

NASA Astrophysics Data System (ADS)

In a system which consisted of luminol (3-aminophthalhydrazide), cobalt sulfate (CoSO 4), alkaline buffer and the mixture of NaSO 3 and sodium bisulfite (NaHSO 3) (sulfite and bisulfite = 3:1, m/m), a strong chemiluminescence (CL) was observed using a BPCL ultra-weak luminometer. The CL signals resulted from 3-aminophthalate (the product of oxidized luminol), and were affected by the buffer pH, buffer medium and the concentrations of luminol, CoSO 4 and the NaSO 3-NaHSO 3 mixture. The observation that the CL intensities were inhibited by superoxide dismutase (SOD), Vitamin C (Vc) and glutathione (GSH) in a dose-dependent manner suggested that superoxide radical (O 2rad -) was involved in the CL reaction and responsible for oxidation of luminol.

Geng, Hong; Meng, Ziqiang

2006-05-01

31

Luminol\\/antibody labeled gold nanoparticles for chemiluminescence immunoassay of carcinoembryonic antigen  

Microsoft Academic Search

A facile strategy by loading luminol and secondary antibody on gold nanoparticles (Au NPs) was described in the present work. The as-prepared luminol\\/antibody labeled Au NPs conjugates (LAAu NPs) were used as the chemiluminescent probe for the detection of carcinoembryonic antigen (CEA) in serum. The LAAu NPs were characterized by transmission electron microscopy (TEM), UV–vis spectrophotometry (UV–vis), and chemiluminescent method.

Xiaoyan Yang; Yingshu Guo; Aiguo Wang

2010-01-01

32

A study of the chemiluminescence behavior of myoglobin with luminol and its analytical applications.  

PubMed

A chemiluminescence signal at 425 nm was observed when ferric state myoglobin was mixed with luminol in alkaline medium. Because the signal was remarkably enhanced in the presence of Fe(CN)6(4-), analytical applications were investigated in a flow-injection system. The increase in chemiluminescence was linearly dependent on myoglobin concentration in the range 0.1 to 100 nmol L(-1), and the limit of detection was 0.04 nmol L(-1) with relative standard deviation 3.2% (3 sigma). It was also found that binding of Mb with the ligands CN-, SCN-, and F- significantly inhibited the chemiluminescence reaction. The linear dynamic ranges for the ligands were 1.0-300.0, 0.1-3.0, and 0.5-100.0 nmol L(-1), and the limits of detection (S/N=3) 0.4, 0.04, and 0.2 nmol L(-1), for F-, CN-, and SCN-, respectively. The relative standard deviations were 5.32%, 6.13%, and 3.38% for 0.1 nmol L(-1) CN-, 0.5 nmol L(-1) SCN-, and 1.0 nmol L(-1) F-, respectively. At a flow rate of 2.0 mL min(-1) the assay could be accomplished in 1 min, including sampling and washing. The method has been successfully applied to the determination of myoglobin in human urine and F- in water samples. A possible mechanism of chemiluminescence production by myoglobin and luminol is presented. PMID:14663544

Song, Zhenghua; Wang, Lin; Hou, Shuang

2003-12-09

33

[The effect of exogenous peroxidase on the intensity of luminol-dependent macrophage chemiluminescence].  

PubMed

The influence of exogenous horse-radish peroxidase on the capacity of mouse peritoneal macrophages to luminol-dependent chemiluminescence induced by zymosan was investigated. It was revealed that peroxidase (1-50 mg/ml) increased the chemiluminescence in a dose-dependent manner. The maximum increase of the response (4-6 times) was obtained with the enzyme concentration being 10 mg/ml. It is found that peroxidase acts as a co-oxidant of the peroxide-dependent extracellular luminol oxidation. The including of the enzyme into macrophages makes it possible to register the intracellular chemiluminescence. PMID:2291242

Tokmakov, A A; Vasil'ev, V Iu

1990-01-01

34

Luminol-enhanced chemiluminescence induced in peripheral blood-derived human phagocytes: obligatory requirement of myeloperoxidase exocytosis by monocytes  

Microsoft Academic Search

Luminol-enhanced chemiluminescence (LCL) of phagocytes is often used to monitor for the generation of reactive oxygen mediators. A strong LCL reaction, as observed in triggered peripheral blood monocytes and neutrophils, depends on both the activation of an NAD(P)H-dependent oxidase and a functional myeloper- oxidase (MP0). The aim of this work was to compare the LCL response induced by soluble and

D. Albrecht; T. W. Jungi

35

A novel chemiluminescence assay of organophosphorous pesticide quinalphos residue in vegetable with luminol detection  

PubMed Central

Background Organophosphorous pesticides are the most popular pesticides used in agriculture. As acetylcholinesterase inhibitors, organophosphorous pesticides are toxic organic chemicals. The control and detection of organophosphorous pesticide residue in food, water, and environment therefore plays a very important role in maintaining physical health. A sensitive, rapid, simple chemiluminescence(CL) method has been developed for the determination of quinalphos based on the reaction of quinalphos with luminol-H2O2 in an alkaline medium. The method has been applied to detection of quinalphos in vegetable samples with satisfactory results. Results The CL method for the determination of organophosphorous pesticide quinalphos is based on the phenomenon that quinalphos can apparently enhance the CL intensity of the luminol-H2O2 system. The optimal conditions were: luminol concentration 5.0 × 10-4 mol/L, H2O2 concentration 0.05 mol/L.pH value 13. In order to restrain the interference from metal ions, 1.0 × 10-3 mol/L of EDTA was added to the luminol solution. The possible mechanism was proposed. Conclusion Under the optimum reaction conditions, CL was linear with the concentration of quinalphos in the range of 0.02 ?g/mL -1.0 ?g/mL and the detection limit was 0.0055 ?g/mL (3?). This method has been successfully applied to the detection of quinalphos in vegetable samples. According to the experimental data, the average recoveries for quinalphos in cherry tomato and green pepper 97.20% and 90.13%. Meanwhile, the possible mechanism was proposed.

2010-01-01

36

Modulatory Role of Nitric Oxide on Superoxide-Dependent Luminol Chemiluminescence  

Microsoft Academic Search

Reactive oxygen species are involved in luminol chemiexcitation induced in biological systems, but the contribution of nitrogen-derived oxidants in the process still remains unclear. Herein, we report that luminol chemiluminescence (LCL) induced by a superoxide[formula]- and hydrogen peroxide (H2O2)-generating system (2–25 mU\\/ml xanthine oxidase plus acetaldehyde and oxygen) was markedly inhibited by nitric oxide ([formula]NO) added either as bolus (0–10

Laura Castro; Mar??a Noel Alvarez; Rafael Radi

1996-01-01

37

Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere  

NASA Astrophysics Data System (ADS)

An instrument has been designed and constructed for the simultaneous determination of nitrogen dioxide (NO2) and peroxyacetyl nitrate (PAN) in atmospheric samples. The instrument's design is based on separation by fast gas chromatography (GC) with a 30 ft capillary column (DB-1) followed by detection by luminol chemiluminescence. The chemiluminescent reaction between NO2 or PAN and luminol takes place at the gas-liquid interface on the surface of a solid support. The chemiluminescent emission at 425 nm is detected with a photon counting module. The instrument is controlled by a 1.8 GHz Notebook computer with a WINDOWS 2000 operating system and a custom software application programmed in LABVIEW. Detection limits are in the low parts per trillion (ppt) with a time resolution of 30 s to 1 min. The instrument was operated during the Mexico City Metropolitan Area/Mexico City Megacity 2003 collaborative air quality study. Results for NO2 from this fast GC method were compared with results from a co-located differential optical absorption spectrometer (DOAS) and a tunable diode laser absorption spectromenter (TDLAS). The results support the application of the new luminol-based instrument for atmospheric measurements.

Marley, Nancy A.; Gaffney, Jeffrey S.; White, Robert V.; Rodriguez-Cuadra, Luis; Herndon, Scott E.; Dunlea, Ed; Volkamer, Rainer M.; Molina, Luisa T.; Molina, Mario J.

2004-11-01

38

Sensitive determination of gentiopicroside in medicine and bio-fluids using luminol-myoglobin chemiluminescence combined with flow injection technique.  

PubMed

A novel chemiluminescence method for the determination of gentiopicroside is presented, which was based on the inhibitory effect of gentiopicroside on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system. The decrement of chemiluminescence intensity was linear with the logarithm of gentiopicroside concentration over the range from 10.0?pg?mL(-1) to 500.0?ng?mL(-1) (r(2) = 0.9992), with a detection limit of 3.0?pg?mL(-1) (3?). At a flow rate of 2.0?mL?min(-1), a complete analytical process could be performed within 0.5?min, including sampling and washing, with a relative standard deviation of less than 3.0% (n = 5). The proposed procedure was applied successfully in the determination of gentiopicroside in pharmaceutical preparations, human urine and serum without any pretreatment procedure. The possible mechanism of the reaction was also discussed. PMID:19743525

He, Xili; Xie, Xiaofeng; Shao, Xiaodong; Song, Zhenghua

39

Assay of picogram level isocarbophos residue on tangerines and oranges with luminol-albumin chemiluminescence system.  

PubMed

A sensitive flow injection-chemiluminescence (FI-CL) method for the determination of isocarbophos (ICP) residue on tangerines and oranges was proposed. It was found that the CL intensity from luminol-albumin CL reaction could be obviously quenched in the presence of ICP and the decrease in CL intensity was proportional to the logarithm of ICP concentrations ranging from 1.0 to 1000 pmol L(-1), giving the limit of detection of 0.3 pmol L(-1) (3?). The proposed procedure was successfully applied to the determination of ICP residue on tangerines and oranges with recoveries varying from 92.0 to 111.0% and RSDs less than 5.0%. The possible CL mechanism of luminol-albumin-ICP reaction was discussed, and ICP to albumin's binding constant (K(D)=1.00 × 10(6) L mol(-1)) and the number of binding sites (n=1.00) were given by the homemade FI-CL model. PMID:22980841

Chen, Donghua; Song, Zhenghua; Lv, Hairu

2012-07-14

40

A study of the chemiluminescence behavior of myoglobin with luminol and its analytical applications  

Microsoft Academic Search

A chemiluminescence signal at 425 nm was observed when ferric state myoglobin was mixed with luminol in alkaline medium. Because the signal was remarkably enhanced in the presence of Fe(CN) 6 4-, analytical applications were investigated in a flow-injection system. The increase in chemiluminescence was linearly dependent on myoglobin concentration in the range 0.1 to 100 nmol L -1, and the limit of

Zhenghua Song; Lin Wang; Shuang Hou

2004-01-01

41

A transition metal enhanced luminol chemiluminescence in the presence of a chelator  

Microsoft Academic Search

We have investigated the chemiluminescence signal of luminol and hydrogen peroxide in the presence of a transition metal (Co(II), Cu(I), Fe(II), Fe(III)) and of a chelator (EDTA, citric acid) in pH 8.5, 9 and 10 borate buffer solutions. We observed that the chemiluminescence intensities of these systems reached a plateau, where they remained stable for a period of 2–30 s.

Irene Parejo; Christos Petrakis; Panagiotis Kefalas

2000-01-01

42

Studies of visible oscillating chemiluminescence with a luminol-H2O2-KSCN-CuSO4-NaOH system in batch reactor.  

PubMed

Oscillating chemical reactions are complex systems involving a large number of chemical species. In oscillating chemical reactions some species, usually reaction intermediates, exhibit fluctuation in concentration. Visible oscillating chemiluminescence, produced by the addition of luminol (3-aminophthalhydrazide) to the oscillating system H(2)O(2)-KSCN-CuSO(4)-NaOH, was investigated. In this study the effect of varying the concentration of H(2)O(2), KSCN, CuSO(4), NaOH and luminol was investigated in a batch reactor. We showed that the concentration of all components involved in the oscillating chemilumenscent reaction influenced the light intensity and the oscillation period. PMID:12536379

Samadi-Maybodi, Abdolraouf; Ourad, S M

43

Effect of gold nanoparticle as a novel nanocatalyst on luminol-hydrazine chemiluminescence system and its analytical application.  

PubMed

In this work the catalytic role of unsupported gold nanoparticles on the luminol-hydrazine reaction is investigated. Gold nanoparticles catalyze the reaction of hydrazine and dissolved oxygen to generate hydrogen peroxide and also catalyze the oxidation of luminol by the produced hydrogen peroxide. The result is an intense chemiluminescence (CL) due to the excited 3-aminophthalate anion. In the absence of gold nanoparticles no detectable CL was observed by the reaction of luminol and hydrazine unless an external oxidant is present in the system. The size effect of gold nanoparticles on the CL intensity was investigated. The most intensive CL signals were obtained with 15-nm gold nanoparticles. UV-vis spectra and transmission electron microscopy studies were used to investigate the CL mechanism. The luminol and hydroxide ion concentration, gold nanoparticles size and flow rate were optimized. The proposed method was successfully applied to the determination of hydrazine in boiler feed water samples. Between 0.1 and 30 microM of hydrazine could be determined with a detection limit of 30 nM. PMID:18291135

Safavi, A; Absalan, G; Bamdad, F

2008-02-02

44

Albumin inhibits human polymorphonuclear leucocyte luminol-dependent chemiluminescence: evidence for oxygen radical scavenging.  

PubMed Central

Luminol-dependent chemiluminescence of normal human polymorphonuclear leucocytes (PMN) which were resting, or stimulated by unopsonized latex beads, opsonized zymosan or the chemotactic peptide N-formyl-met-leu-phe was decreased more than 80% in the presence of physiological concentrations of albumin (4%, w/v). This inhibition did not result from impairment of light transmission, cellular toxicity, luminol excited-state quenching or a dialysable contaminant in the albumin preparation, but was reduced by 30% when the fall induced by albumin in extracellular free Ca2+ concentration was corrected. The inhibition was most apparent in the larger second phase of the PMN chemiluminescent response to chemotactic peptide or opsonized zymosan stimulation. The smaller first phase of these responses was in fact enhanced by low concentrations of albumin (0.05-0.5%, w/v) and only inhibited up to 50% by 4% (w/v) albumin. Albumin in the range 0.1-4% (w/v) exerted a similar effect on chemiluminescence resulting from superoxide anion (O-2) and hydrogen peroxide (H2O2) production by xanthine oxidase catalysed oxidation of xanthine in the presence of luminol. We suggest that the effect of albumin on PMN luminol-dependent chemiluminescence is mediated by modification of the oxygen radical generating pathway, or oxygen radical scavenging. This previously undocumented property of the major extracellular protein requires further examination if oxygen radicals are to be established as important mediators of inflammation.

Holt, M. E.; Ryall, M. E.; Campbell, A. K.

1984-01-01

45

Application of response surface methodology (RSM) to the optimization of a post-column luminol chemiluminescence analysis of silyl peroxides.  

PubMed

The possibility of the utilization of chemiluminescence post-column luminol oxidation (CL) in a HPLC system for silyl peroxides analysis has been investigated. The conditions of HPLC separation for 12 silyl peroxides, representing bissilyl and alkyl-silyl peroxides, as well as their potential impurities, were established. Optimal chemiluminescent post-column reaction conditions were found using central composite design (CCD) and response surface methodology (RSM). The interaction effects of four of the most important operating variables - the concentrations of luminol, hemin, sodium hydroxide and the post-column solution flow rate - on the light intensity were evaluated. The optimized conditions for analysis were the same for bissilyl and alkyl-silyl peroxides for the base concentration (0.03 M), the luminol concentration (0.4 g L(-1)) and the hemin concentration (0.3 g L(-1)). The only differences occurred in a reagent flow rate (for bissilyl peroxide -0.3 mL min(-1) and for alkyl-silyl peroxides -0.9 mL min(-1)). Under optimal conditions, the detection limits were in the 0.07-0.16 nM range for bissilyl, and 0.53-1.01 for alkyl-silyl peroxides. The calibration curves were linear in the 0.25-3 nM range for bissilyl and the 2.5-25 range for alkyl-silyl peroxides. Intra-day and inter-day precision was lower than 5.5% for each tested concentration level. A mechanism of luminol oxidation by silyl peroxides involving a hydrolysis step with the formation of hydrogen peroxide or hydroperoxide was proposed. PMID:23200374

Baj, Stefan; S?upska, Roksana; Krawczyk, Tomasz

2012-10-12

46

Investigations of different chemiluminescent peaks in H2O2-SCN(-)-Cu(2+)-OH(-)-luminol flow system.  

PubMed

In the H2O2-SCN(-) -Cu(2+)-OH(-)-luminol oscillatory system of chemiluminescence, the effects of the ingredient concentrations, temperature, flow rate and complexing agent on the oscillatory dynamics were investigated in a continuous-flow stirred tank reactor (CSTR). The dynamical structure of two peaks during a period was discussed in detail. By addition of EDTA to the oscillating system, the peak I height decreased sharply while the peak II height was little affected, and the period kept constant. This may be due to the fast reaction between Cu(II) and EDTA and the highly stable complex Cu(II)-EDTA. From the experimental study and mechanism analysis, the chemiluminescent peak I corresponds to Cu(II) ? Cu(I) transformation and the peak II corresponds to the Cu(I) ? Cu(II) transformation process. The key species involving in the two-transformation process are inferred to be superoxide radical and hydroxyl radical. PMID:21491564

Zhao, Changchun; Zheng, Juhua; Xie, Jingxuan; Liu, Haimiao; Gao, Qingyu

47

Stimulation of alveolar macrophages by mineral dusts in vitro: luminol-dependent chemiluminescence study  

SciTech Connect

Luminol-dependent chemiluminescence (CL) of normal (nonactivated) rabbit alveolar macrophages (AMs) was measured in suspension upon stimulation by various size fractions of one quartz dust sample or by various mineral dusts (quartz, corundum, anatas, and chrysotile asbestos as an example of fibrous dust). The CL-triggering capacity of the tested dusts was inhibited by their preincubation with autologous serum. The intensity of luminol-dependent CL induced by particulate dusts upon their action on AMs depended on the kind of dust, on the dust particle sizes, and on the ratio of the number of particles to the number of cells in a given suspension. The cytotoxicity and/or fibrogenicity of the dust and its capacity to trigger the luminol-dependent CL of nonadherent AMs were not directly correlated.

Vilim, V.; Wilhelm, J.; Brzak, P.; Hurych, J.

1987-02-01

48

A Myeloperoxidase-Specific Assay Based upon Bromide-Dependent Chemiluminescence of Luminol  

Microsoft Academic Search

Measurement of myeloperoxidase (MPO; EC 1.11.1.7) activity is often used as a marker of neutrophil infiltration into tissues. However, most enzymatic assays for MPO are susceptible to interference from other peroxidases (including eosinophil peroxidase, EPX) and hemoproteins (such as hemoglobin and myoglobin) present in the tissues. In this report, we describe a bromide-dependent chemiluminescence (Br-CL) assay that uses luminol as

Arsalan S. Haqqani; Jagdeep K. Sandhu; H. Chaim Birnboim

1999-01-01

49

Effects of pteridines on luminol-dependent chemiluminescence induced by chloramine-T  

Microsoft Academic Search

Pteridines are ubiquitous in living organisms, but little is known about their biological functions. Different pteridines were tested for their ability to modulate luminol-dependent chemiluminescence induced by chloramine-T at pH = 7.5 and at a concentration of 100 ?M for each pteridine. We observed striking differences between the compounds; whereas reduced pteridine species were generally potent scavengers, aromatic pteridines were

Gilbert Reibnegger; Dietmar Fuchs; Christian Murr; Manfred P. Dierich; Wolfgang Pfleiderer; Helmut Wachter

1995-01-01

50

Evaluation of total antioxidant potential (TRAP) and total antioxidant reactivity from luminol-enhanced chemiluminescence measurements  

Microsoft Academic Search

The effect of antioxidants and biological fluids on the intensity of luminol induced chemiluminescence by radicals derived from the thermolysis of 2,2?-azo-bis(2-amidinopropane) has been employed to monitor TRAP and TOTAL ANTIOXIDANT REACTIVITY (TAR) levels. The latter parameter, which considers not only the quantity of oxidants but also their reactivity, is considered a potentially more useful index of the antioxidant status

Eduardo Lissi; Marta Salim-Hanna; Carlos Pascual; Maria D. del Castillo

1995-01-01

51

A novel luminol chemiluminescent method catalyzed by silver/gold alloy nanoparticles for determination of anticancer drug flutamide.  

PubMed

It was found that silver/gold alloy nanoparticles enhance the chemiluminescence (CL) of the luminol-H2O2 system in alkaline solution. The studies of UV-Vis spectra, CL spectra, effects of concentrations luminol, hydrogen peroxide and silver/gold alloy nanoparticles solutions were carried out to explore the CL enhancement mechanism. Flutamide was found to quench the CL signals of the luminol-H2O2 reaction catalyzed by silver/gold alloy nanoparticles, which made it applicable for the determination of flutamide. Under the optimum conditions, the CL intensity is proportional to the concentration of the flutamide in solution over the range 5.0×10(-7) to 1.0×10(-4)molL(-1). Detection limit was obtained 1.2×10(-8)molL(-1)and the relative standard deviation (RSD) ?5%. This work is introduced as a new method for the determination of flutamide in commercial tablets. Box-Behnken experimental design is applied to investigate and validate the CL measurement parameters. PMID:23978744

Chaichi, Mohammad Javad; Azizi, Seyed Naser; Heidarpour, Maryam

2013-08-08

52

Effects of pH and Surfactant on the Ultrasound-Induced Chemiluminescence of Luminol  

NASA Astrophysics Data System (ADS)

The pH dependence of the sonochemi-luminescence (SCL) of luminol at 141 kHz was investigated and compared to the effect of pH on its fluorescence emission intensity. From the fluorescence intensity, the pKa1* and the pKa2* values were determined to be 6.4 and 10.5 respectively, which was consistent with changes observed in the SCL intensity of luminol when the pH was varied. An inverse relationship was found to exist between the SCL and the fluorescence intensities against pH, i.e., the luminol SCL spectrum at pH=10.8 (mono-anionic form) had the same shape as the fluorescence emission spectra in acidic pH solutions, where luminol exists in either the mono-cationic or neutral form. The addition of an anionic surfactant, sodium dodecyl sulfate (SDS), significantly decreased the luminol SCL intensity at high pH@. It is proposed that the generation of a negative electric potential at the surface of cavitation bubbles effectively prevented the negatively charged form of luminol from approaching the bubbles, thereby inhibiting the reaction of luminol with OH radicals.

Miyoshi, Norio; Hatanaka, Shin-ichi; Yasui, Kyuichi; Mitome, Hideto; Fukuda, Masaru

2001-06-01

53

The forensic use of luminol chemiluminescence to detect traces of blood inside motor vehicles.  

PubMed

The luminol test for blood was carried out on a set of interior fittings and surfaces inside three different makes of modern motor car. The surfaces and fittings provided little interference with the test for blood, although there was some detectable chemiluminescence when the test was applied to blood-free material from a seatbelt, a boot-lining and a gear-knob. The case with which haemoglobin samples could be washed off interior car surfaces was also examined for seat fabrics, carpets, roof-linings and various other plastic interior surfaces. A standard wash with water alone was not very effective and removed only ca. 50% of the haemoglobin. A standard wash with soapy water or with a proprietary multipurpose car cleaner removed ca. 90% of the haemoglobin from the tested surface. The effect of high car interior temperatures on haemoglobin samples that were subsequently used in the luminol test was also examined. It was shown that the sensitivity of the luminol test was not decreased but was increased by the prior heating of a haemoglobin sample. This effect was attributed to the thermal conversion of haemoglobin to the more brighter catalyst for chemiluminescence, methaemoglobin. The enthalpy of this conversion in the solid state was found to be 14.1 kJ/mol. PMID:15449350

Quickenden, T I; Ennis, C P; Creamer, J I

54

The combined luminol/isoluminol chemiluminescence method for differentiating between extracellular and intracellular oxidant production by neutrophils.  

PubMed

To address the question why isoluminol, but not luminol, failed to detect oxidants produced intracellularly, differences between these luminophores were investigated with respect to physicochemical parameters and the character of chemiluminescence signal. Our results showed the isoluminol molecule to be more polar, more hydrophilic and possessing lower ability to form intramolecular bonds than the luminol molecule. Therefore, isoluminol: (i) only slightly pervaded biological membranes; (ii) depended essentially on extracellular peroxidase; (iii) did not produce chemiluminescence in the presence of extracellular scavengers; and (iv) it could be considered a specific detector of extracellular radicals. On the other hand, the physicochemical parameters of luminol and partial resistance of its chemiluminescence to the effect of extracellular inhibitors proved the lipo/hydrophilic character of this luminophore and thus its ability to interact with radicals both outside and inside of cells. The luminol chemiluminescence measured in the presence of extracellular scavengers and the isoluminol chemiluminescence were used with the intention to differentiate the effects of two antihistamine drugs on intra- and extracellular radical formation. In activated human neutrophils, brompheniramine inhibited the extracellular and potentiated the intracellular part of chemiluminescence signal, whereas a reducing effect of loratadine was observed in both compartments. PMID:16805965

Jancinová, Viera; Drábiková, Katarína; Nosál, Radomír; Racková, Lucia; Májeková, Magdaléna; Holománová, Dagmar

2006-01-01

55

Development and optimization of an analytical method for the determination of Sudan dyes in hot chilli pepper by high-performance liquid chromatography with on-line electrogenerated BrO ?-luminol chemiluminescence detection  

Microsoft Academic Search

The determination of four Sudan dyes by means of high-performance liquid chromatography (HPLC) with chemiluminescence (CL) detection was proposed. The method was based on the enhancement effect of Sudan dyes on the chemiluminescence reaction between luminol and BrO?, which was on-line electrogenerated by constant current electrolysis. The separation was carried out on Nucleosil RP-C18 column (250mm×4.6mm i.d., 5?m, pore size,

Yantu Zhang; Zhujun Zhang; Yonghua Sun

2006-01-01

56

Study on the chemiluminescence resonance energy transfer between luminol and fluorescent dyes using a linear CCD spectrometer  

Microsoft Academic Search

Chemiluminescence (CL) has been a useful tool for analytical applications. Fluorescein can be used to enhance CL emission of luminol. With three-dimensional (3D) dynamic CL spectrum obtained from a linear CCD (charge-coupled device) flow-injection CL spectrometer, the fluorescein enhanced CL analysis was studied. The enhanced process can be described with chemiluminescence resonance energy transfer (CRET) at certain fluorescein concentrations. In

Hao Xu; Chun-Mei Liu; Yue He; Hong-Wu Tang; Qiong-Shui Wu

2010-01-01

57

Effects of non-steroidal anti-inflammatory drugs on the luminol and lucigenin amplified chemiluminescence of human neutrophils  

Microsoft Academic Search

A panel of non-steroidal anti-inflammatory drugs commonly used for therapeutic purposes was assessed for their effects on the respiratory burst of isolated human polymorphonuclear neutrophils. Cells were stimulated with opsonised yeast and the production of reactive oxygen species was measured by amplified chemiluminescence with luminol and lucigenin which are two luminogenic agents measuring different cellular events. A special attention was

Nathalie Parij; Anne-Marie Nagy; Pierre Fondu; Jean Nève

1998-01-01

58

Determination of dissolved Fe(II) in seawater of the western North Pacific with luminol chemiluminescence method  

Microsoft Academic Search

Determination of dissolved Fe(II) in seawater of the western North Pacific with luminol chemiluminescence method Hajime Obata, Akira Mase, Toshitaka Gamo (Atmosphere and Ocean Research Institute, University of Tokyo, Japan), Jun Nishioka (Institute of Low Temperature Science, Hokkaido University, Japan), Shigenobu Takeda (Faculty of Fisheries, Nagasaki University, Japan) Speciation of iron in the ocean is now important topics because the

H. Obata; A. Mase; T. Gamo; J. Nishioka; S. Takeda

2010-01-01

59

Contribution of nitric oxide synthase to luminol-dependent chemiluminescence generated by phorbol-ester-activated Kupffer cells.  

PubMed Central

Phorbol 12-myristate 13-acetate-induced luminol chemiluminescence in rat Kupffer cells was doubled by the addition of L-arginine and significantly (up to 70%) inhibited by NG-nitro-L-arginine and NG-monomethyl-L-arginine, competitive inhibitors of L-arginine-dependent nitric oxide (NO) formation. The release of superoxide anion (O2-) by NADPH oxidase was neither affected by L-arginine nor by the inhibitors. Only very slight luminol chemiluminescence was detectable in lipopolysaccharide-pretreated Kupffer cells, a condition in which significant amounts of NO were formed but no O2-. In a cell-free system, significant luminol chemiluminescence only occurred when both authentic NO and the O2-/H2O2- generating system xanthine/xanthine oxidase were present. The results indicate that luminol chemiluminescence in phorbol-ester-activated Kupffer cells largely depends on L-arginine metabolism by NO synthase, requiring the concurrent formation of NO and O2-/H2O2.

Wang, J F; Komarov, P; Sies, H; de Groot, H

1991-01-01

60

Determination of nitrogen dioxide with a chemiluminescent aerosol detector  

SciTech Connect

A modified detector is described for use in the determination of nitrogen dioxide via reaction with luminol. Chemiluminescence of the aerosol particles formed by crossed streams of the analyte and an alkaline luminol solution was observed by a photomultiplier.

Mikuska, P.; Vecera, Z. [Institute of Analytical Chemistry, Brno (Czechoslovakia)

1992-09-15

61

Capillary gas chromatographic analysis of nitrogen dioxide and pans with luminol chemiluminescent detection.  

SciTech Connect

Peroxyacyl nitrates (PANs) and nitrogen dioxide are important atmospheric air pollutants in the troposphere. These atmospheric nitrogen species are strongly coupled chemically by a clearly temperature-dependent equilibrium in the troposphere. A chemical method that can measure both nitrogen dioxide and PANs rapidly and with sub-part-per-billion detection is described that is based upon a modified luminol detection system coupled to a capillary gas chromatographic column by using helium as a carrier. The system can readily separate and detect nitrogen dioxide, peroxyacetyl nitrate, peroxyproprionyl nitrate, and peroxybutyrl nitrate with detection limits in the low tens of parts per trillion with total analysis time of less than 1 min. Calibration of PAN by thermal decomposition to nitrogen dioxide is demonstrated with PAN detection sensitivities approximately 75% of the sensitivities observed for NO2 luminol detection by using helium as a carrier gas. The advantages of this method for simultaneous measurement of nitrogen dioxide and PANs over ozone chemiluminescent detection and electron capture detection are discussed, as well as potential applications of this method for heterogeneous surface chemistry studies of PANs and nitrogen dioxide and for tropospheric measurements.

Gaffney, J. S.; Bornick, R. M.; Chen, Y.-H.; Marley, N. A.; Environmental Research

1998-01-01

62

Enzymeless determination of total sugar by luminol-tetrachloroaurate chemiluminescence on chip to analyze food samples.  

PubMed

Chemiluminescence (CL) emission from luminol-tetrachloroaurate ([AuCl(4)](-)) system studied in presence of monosaccharide sugars such as glucose and fructose was investigated on a microfluidic chip fabricated by the soft lithography technique. CL emission from the luminol-[AuCl(4)](-) system at 430 nm was intensified remarkably by the catalytic activity of glucose and fructose at room temperature. Under optimized conditions, the CL emission intensity of the system was found to be linearly related to the concentration of the sugars. Based on this observation, nonenzymatic determination of total sugar (glucose, fructose, or hydrolyzable sucrose) was performed in a rapid and sensitive analytical method. The results revealed that the linearity ranged from 9 to 1,750 ?M for glucose and 80 to 1,750 ?M for fructose, with a limit of detection of 0.65 and 0.69 ?M, respectively. The relative standard deviations determined at 250 ?M based on six repetitive injections were 1.13 and 1.15% for glucose and fructose, respectively. The developed method was successfully applied for determination of the total sugar concentration in food and beverages. PMID:23052873

Alam, Al-Mahmnur; Kamruzzaman, Mohammad; Dang, Trung-Dung; Lee, Sang Hak; Kim, Young Ho; Kim, Gyu-Man

2012-10-04

63

Evaluation of scavenging activity assessed by Co(II)\\/EDTA-induced luminol chemiluminescence and DPPH · (2,2-diphenyl-1-picrylhydrazyl) free radical assay  

Microsoft Academic Search

The scavenging activities of three standard antioxidants, quercetin, ascorbic acid, and trolox, were evaluated by Co(II)\\/ethylenediamine-tetraacetic acid (EDTA)-induced luminol chemiluminescence and the 2,2-diphenyl-1-picrylhydrazyl (DPPH·) free radical assay. Therefore, the aim of this study was to characterise an enzyme-free and time-independent chemiluminescence method for the assessment of the scavenging profile of compounds in a cell-free system using the Co(II)\\/EDTA–luminol–peroxide system. These

Irene Parejo; Carles Codina; Christos Petrakis; Panagiotis Kefalas

2000-01-01

64

Investigation of the radical step in the chemiluminescent oxidation of luminol by potassium ferricyanide in the presence of hydrogen peroxide with the aid of magnetic modulation  

SciTech Connect

A method employing magnetic modulation of the rate of the chemical reaction was previously applied with success to the establishment of the chemiluminescent reaction involving the oxidation of luminol (LH/sub 2/) by potassium ferricyanide K/sub 3/Fe-(CN)/sub 6/ in an aqueous solution of sodium hydroxide (0.1 M NaOH). The purpose of the present work was to experimentally investigate the radical steps of the more complex chemiluminescent reaction involving the oxidation of LH/sub 2/ in the presence of H/sub 2/O/sub 2/. The action of the magnetic field on the reaction causes an increase in the intensity of the chemiluminescence. The dependence of the intensity of the chemiluminescence on the magnetic field strength has the form of a saturation curve. The hyperfine interaction of the electronic and nuclear spins of the recombining radicals create a possibility for the mixing of the singlet and triplet states of the radical pairs, which results in relative alteration of the populations of these states with the rate constant K/sub st/(H). An external magnetic field reduces this constant, causing an increase in the concentration of the LOH/sup 2 -/ radicals and, consequently, an increase in the output of light.

Tribel', M.M.; Morozov, A.K.; Frankevich, E.L.

1987-11-01

65

Development of a highly sensitive chemiluminescence enzyme immunoassay using enhanced luminol as substrate.  

PubMed

In this study, a high sensitivity chemiluminescence enzyme immunoassay (CLEIA) based on novel enhancers was developed. Under optimal conditions, we developed an enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP-C) in the presence of 3-(10'-phenothiazinyl) propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORP) as enhancers. The limit of detection of the newly prepared chemiluminescent cocktail for HRP was 0.33?pg/well, which is lower than that of commercial Super Signal substrate. The results showed that this novel chemiluminescent cocktail can significantly increase the light output of HRP-catalyzed ECR, which can be translated into a corresponding improvement in sensitivity. Similar improvements were observed in CLEIA for the determination of chloramphenicol in milk. In addition, the ECR of N-azoles as secondary enhancer was also presented. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23785024

Tao, Xiaoqi; Wang, Wenjun; Wang, Zhanhui; Cao, Xingyuan; Zhu, Jinghui; Niu, Lanlan; Wu, Xiaoping; Jiang, Haiyang; Shen, Jianzhong

2013-06-20

66

Drugs effects on superoxide generation and chemiluminescence response of human leukocytes  

Microsoft Academic Search

Luminol-enhanced chemiluminescence was used to determine the effects of diethyldithiocarbamate, dipyridamole, catechin and verapamil on the generation of reactive oxygen species in human leukocytes, and on superoxide generated by chemiluminescence of the hypoxanthine xanthine-oxidase reaction. These agents reduced the luminol enhanced chemiluminescence response of activated leukocytes, most probably by inhibiting the superoxide generation reaction. On the other hand, citrate and

C. Pascual; R. González; C. Romay

1991-01-01

67

Luminol oxidation by hydrogen peroxide with chemiluminescent signal formation catalyzed by peroxygenase from the fungus Agrocybe aegerita V.Brig  

Microsoft Academic Search

Conditions of luminol oxidation by hydrogen peroxide in the presence of peroxygenase from the mushroom Agrocybe aegerita V.Brig. have been optimized. The pH value (8.8) at which fungal peroxygenase produces a maximum chemiluminescent signal has\\u000a been shown to be similar to the pH optimum value of horseradish peroxidase. Luminescence intensity changed when the concentration\\u000a of Tris-buffer was varied; maximum intensity

M. M. Vdovenko; R. Ullrich; M. Hofrichter; I. Yu. Sakharov

2010-01-01

68

Chemiluminescent lipase determination based on the enhanced luminol\\/H 2 O 2 \\/horseradish peroxidase\\/fluorescein diacetate energy transfer system  

Microsoft Academic Search

A methodology for the determination of lipase, based on the coupled processes of energy transfer and enhancement of the chemiluminescence\\u000a of the luminol-H2O2-horseradish peroxidase (HRP) system has been developed. Fluorescein diacetate (FDA) was hydrolyzed to fluorescein by the\\u000a action of the enzyme lipase, and this compound acted as an enhancer of the chemiluminescent process and acceptor of the chemiluminescent\\u000a emission

A. Navas Díaz; F. G. Sanchez; M. C. Torijas; J. Lovillo

1999-01-01

69

Chemiluminescent Reactions Catalyzed by Nanoparticles of Gold, Silver, and Gold/Silver Alloys  

NASA Astrophysics Data System (ADS)

Chemiluminescence (CL) reactions are catalyzed by metals nanoparticles, which display unique catalytic properties due to an increased surface area. The present study describes the catalytic effects of nanoparticles (NP) of silver, gold, and alloys of Au/Ag nanoparticles on the chemiluminescent reaction taking place between luminol and potassium ferricyanide. It was found that silver nanoparticles and alloy nanoparticles enhance the CL process when their sizes remained in the range of 30 nm to 50 nm. The data show that the intensity and rate of chemiluminescence were influenced by the mole fraction of gold and silver in the alloy. Data to this chemiluminescence reaction are modeled by a double exponential curve, which indicates that two competing processes are occurring.

Abideen, Saqib Ul

70

Analytical assessment of the oscillating chemical reactions by use chemiluminescence detection  

Microsoft Academic Search

This paper introduces the chemiluminescence (CL) detection in oscillating reaction-based determinations using the analyte pulse perturbation technique, a straightforward and expeditious approach to deriving quantitative analytical information from oscillating chemical reactions. The behavior of the H2O2–KSCN–CuSO4-NaOH oscillating system in the presence of luminol was examined by using the proposed detection method and the classical potentiometric technique. Some analytical and practical

Rafael Jiménez-Prieto; Manuel Silva; Dolores Pérez-Bendito

1997-01-01

71

Enhanced chemiluminescence of the luminol-hydrogen peroxide system by colloidal cupric oxide nanoparticles as peroxidase mimic.  

PubMed

As a peroxidase mimic, cupric oxide nanoparticles were found to enhance the chemiluminescence (CL) of luminol-H(2)O(2) system up to 400 folds. The CL spectra and radical scavengers were conducted to investigate the possible CL enhancement mechanism. It was suggested that the enhanced CL could be attributed to the peroxidase-like activity of CuO nanoparticles, which effectively catalyzed the decomposition of hydrogen peroxide into hydroxyl radicals. The effects of the reactant concentrations and some organic compounds were also investigated. The proposed method could be used as a sensitive detection tool for hydrogen peroxide and glucose. PMID:22967606

Chen, Wei; Hong, Lei; Liu, Ai-Lin; Liu, Jian-Qing; Lin, Xin-Hua; Xia, Xing-Hua

2012-06-29

72

Study of the oscillation and luminol chemiluminescence in the H 2O 2-KSCN-CuSO 4-NaOH system  

NASA Astrophysics Data System (ADS)

Oscillations in redox potential and chemiluminescence of the H 2O 2-KSCN-CuSO 4-NaOH system in the presence of luminol were examined. Parts of the mechanism proposed in the previous studies were evaluated by substitution of SCN - with CN -. The amplitude of the chemiluminescent oscillations was found to be strongly dependent on the initial luminol concentrations. In addition, the time-series ARMA (2;1)-analysis with Box-Jenkins algorithm were used to simulate the system and the result is well in accordance with the observed oscillations.

Kiatisevi, Supavadee; Maisch, Steffen

2010-10-01

73

A kinetic treatment of stopped-flow time courses for multiple chemiluminescence of the KIO4-luminol-Mn2+ system.  

PubMed

Stopped-flow time courses for chemiluminescence (CL) of the KIO4-luminol-Mn(2+) system showed an instantaneous jump in initial signal followed by two distinct bands. A kinetic model of the form [formula in text] with ten adjustable parameters was proposed to account for CL intensity (I) versus time (t) profiles. The three terms in the model represent the three CL bands. Each band was comprised of a rise part and an exponential decay corresponding to the formation and deactivation of the CL emitter. CL bands could have originated from different CL pathways with the participation of reactive species such as O2(-), (•)OH and (1)O2 generated in the reactions involving IO4(-), O2 and Mn(2+). Subsequent reactions of these reactive species with luminol induced CL emissions. Simulation parameters together with peak positions and intensities of the three CL bands were found to vary in different manners by changing conditions such as reagent concentration, pH and temperature. The temperature-dependence of the rate constants yielded activation energies of 73.2 ± 2.8, 70.1 ± 2.4 and 67.2 ± 1.2 kJ mol(-1) for the three decay processes. Moreover, different substances exhibited a significant influence on the three CL bands and their simulation parameters. The numerous parameters and characteristics of CL emissions could serve as multiple probes for detecting analytes, making this system promising for potential analytical applications. PMID:22764106

Ma, Ai-Jay; Chang, Yu-Tang; Lin, Wann-Yin

2012-07-05

74

Chemiluminescent detection of organic air pollutants  

SciTech Connect

Chemiluminescent reactions can be used for specific and highly sensitive detection of a number of air pollutants. Among these are chemiluminescent reactions of ozone with NO or organics and reactions of luminol with a variety of oxidants. Reported here are studies exploring (1) the use of the temperature dependence of the chemiluminescent reactions of ozone with organic pollutants as a means of differentiating types of hydrocarbon classes and (2) the use of luminol techniques to monitor atmospheric concentrations of nitrogen dioxide (NO{sub 2}) and organic oxidants, specifically peroxyacyl nitrates (PANs). Coupling gas chromatography to the chemiluminescent detectors allows the measurement of individual species at very low concentrations.

Marley, N.A.; Gaffney, J.S.; Chen, Yu-Harn

1996-04-01

75

On-line selective detection of antioxidants free-radical scavenging activity based on Co(II)\\/EDTA-induced luminol chemiluminescence by flow injection analysis  

Microsoft Academic Search

This study establishes a new method to analyze the radical scavenging activity of antioxidants based on the luminol-H2O2-Co(II)\\/EDTA chemiluminescence and flow injection analysis. The method is based on the catalytic oxidation of hydrogen peroxide by Co(II)\\/EDTA complex, forming a free radical flux that can produce a stable chemiluminescence signal which is attenuated in the presence of antioxidants. A properly designed

Dimosthenis L. Giokas; Athanasios G. Vlessidis; Nicholaos P. Evmiridis

2007-01-01

76

Flow injection analysis of ketoprofen based on the order transform second chemiluminescence reaction  

NASA Astrophysics Data System (ADS)

This paper explores an order-transform-second-chemiluminescence (OTSCL) method combining the flow injection technique for the determination of ketoprofen. When ketoprofen solution was injected into the mixture after the end of the reaction of alkaline luminol and sodium periodate or sodium periodate solution was injected into the reaction mixture of ketoprofen and alkaline luminol, a new chemiluminescence (CL) reaction was initiated and strong CL signal was detected. A mechanism for the OTSCL has been proposed on the basis of the chemiluminescence kinetic characteristic, UV-visible absorption and chemiluminescent spectra. Under optimal experimental conditions, the CL response is proportional to the concentration of ketoprofen over the range of 2.0 × 10 -7 to 1.0 × 10 -5 mol/L with a correlation coefficient of 0.9950 and a detection limit of 8.0 × 10 -9 mol/L (3 ?). The relative standard deviation for 11 repetitive determinations of 1.0 × 10 -6 mol/L ketoprofen is 2.9%. The utility of this method was demonstrated by determining ketoprofen in pharmaceutical formulations without interference from its potential impurities.

Zhuang, Yafeng; Cao, Guiping; Ge, Chuanqin

2012-01-01

77

Increased production of luminol enhanced chemiluminescence by the inflamed colonic mucosa in patients with ulcerative colitis  

Microsoft Academic Search

Reactive oxygen species have been implicated as mediators of inflammation in ulcerative colitis. Chemiluminescence is a reliable means of estimating reactive oxygen species in biological media. Increased reactive oxygen species values in the inflamed colonic mucosa in rats were seen by chemiluminescence. The aims of the study were to find out if chemiluminescence is raised in the colonic mucosa of

S Sedghi; J Z Fields; M Klamut; G Urban; M Durkin; D Winship; D Fretland; M Olyaee; A Keshavarzian

1993-01-01

78

Study on the reaction mechanism and the static injection chemiluminescence method for detection of acetaminophen.  

PubMed

Acetaminophen, also called paracetamol, is found in Tylenol, Excedrin and other products as over-the-counter medicines. In this study, acetaminophen as a luminol signal enhancer was used in the chemiluminescence (CL) substrate solution of horseradish peroxidase (HRP) for the first time. The use of acetaminophen in the luminol-HRP-H(2) O(2) system affected not only the intensity of the obtained signal, but also its kinetics. It was shown that acetaminophen was to be a potent enhancer of the luminol-HRP-H(2) O(2) system. A putative enhancement mechanism for the luminol-H(2) O(2) -HRP-acetaminophen system is presented. The resonance of the nucleophilic amide group and the benzene ring of acetaminophen structure have a great effect on O-H bond dissociation energy of the phenol group and therefore on phenoxyl radical stabilization. These radicals act as mediators between HRP and luminol in an electron transfer reaction that generates luminol radicals and subsequently light emission, in which the intensity of CL is enhanced in the presence of acetaminophen. In addition, a simple method was developed to detect acetaminophen by static injection CL based on the enhanced CL system of luminol-H(2) O(2) -HRP by acetaminophen. Experimental conditions, such as pH and concentrations of substrates, have been examined and optimized. The proposed method exhibited good performance, the linear range was from 0.30 to 7.5 mM, the relative standard deviation was 1.86% (n?=?10), limit of detection was 0.16 mM and recovery was 99?±?4%. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23408702

Wu, Yongjun; Zhang, Huili; Yu, Songcheng; Yu, Fei; Li, Yanqiang; Zhang, Hongquan; Qu, Lingbo; Harrington, Peter de B

2013-02-14

79

[Determining the postmortem interval of bone samples: a comparison of luminol chemiluminescence, Hexagon OBTI test, and Combur test].  

PubMed

In the experiment, 16 human bones with known postmortem interval (PMI) that had been buried in soil (0.2 to about 2000 years) were tested in a blind setup with two established methods for determining the PMI (UV fluorescence of the surface of a fresh cut and the luminol chemiluminescence) and with two methods applied for this purpose for the first time (Hexagon OBTI test and Combur test). The results underline the importance of the UV fluorescence and luminol tests in determining the PMI, especially with regard to the question whether the PMI is forensically relevant or not. The results for both new methods, the Combur test strips and the Hexagon OBTI test, which were originally developed for the detection of hemoglobin, were negative for all samples. It remains to be seen if the negative results for these two methods may be due to an inability of hemoglobin or its metabolites to dissolve in the Tris buffer solution used in the experiment. PMID:20806675

Ebach, Sarah C; Ramsthaler, Frank; Birngruber, Christoph G; Verhoff, Marcel A

80

Enhanced effect of aggregated gold nanoparticles on luminol chemiluminescence system and its analytical application  

NASA Astrophysics Data System (ADS)

Some organic compounds containing groups of OH, NH2, or SH, which could induce the aggregation of gold nanoparticles (AuNPs), were observed to enhance effectively the luminol-H2O2-2.6 nm AuNPs CL system. It was found that the aggregation of AuNPs was an important effect factor for the catalytic activity of AuNPs on luminol CL system. The aggregated AuNPs could effectively enhance luminol CL signal compared with the dispersed one. The enhanced effect was closely related to the sizes of AuNPs. Among the studied AuNPs with seven sizes, 2.6 nm AuNPs had the greatest enhancement effect on luminol CL system after its aggregation. The CL enhancement mechanism was investigated, and the marked enhancement of aggregated 2.6 nm AuNPs for luminol CL system was supposed to originate from the decrease of AuNPs' surface negative charge density compared to its dispersed state. For the luminol-H2O2-2.6 nm AuNPs CL system in the presence of organic compounds containing groups of OH, NH2, or SH, more than one factor played the role in influencing the CL intensity. It was found that the enhanced effect of aggregated 2.6 nm AuNPs induced by such organic compounds was much more significant than the inhibition effect of reducing groups of OH, NH2, or SH, which made it applicable for the determination of this kind of compounds.

Qi, Yingying; Li, Baoxin

2013-07-01

81

Enhanced effect of aggregated gold nanoparticles on luminol chemiluminescence system and its analytical application.  

PubMed

Some organic compounds containing groups of OH, NH2, or SH, which could induce the aggregation of gold nanoparticles (AuNPs), were observed to enhance effectively the luminol-H2O2-2.6 nm AuNPs CL system. It was found that the aggregation of AuNPs was an important effect factor for the catalytic activity of AuNPs on luminol CL system. The aggregated AuNPs could effectively enhance luminol CL signal compared with the dispersed one. The enhanced effect was closely related to the sizes of AuNPs. Among the studied AuNPs with seven sizes, 2.6 nm AuNPs had the greatest enhancement effect on luminol CL system after its aggregation. The CL enhancement mechanism was investigated, and the marked enhancement of aggregated 2.6 nm AuNPs for luminol CL system was supposed to originate from the decrease of AuNPs' surface negative charge density compared to its dispersed state. For the luminol-H2O2-2.6 nm AuNPs CL system in the presence of organic compounds containing groups of OH, NH2, or SH, more than one factor played the role in influencing the CL intensity. It was found that the enhanced effect of aggregated 2.6 nm AuNPs induced by such organic compounds was much more significant than the inhibition effect of reducing groups of OH, NH2, or SH, which made it applicable for the determination of this kind of compounds. PMID:23602952

Qi, Yingying; Li, Baoxin

2013-03-13

82

Aircraft measurements of nitrogen dioxide and peroxyacetyl nitrates using luminol chemiluminescence with fast capillary gas chromatography  

Microsoft Academic Search

Fast capillary gas chromatography with luminol detection has been used to make airborne measurements of nitrogen dioxide (NOâ) and peroxyacetyl nitrate (PAN). The analysis system allows for the simultaneous measurement of NOâ and peroxyacyl nitrates (PANs) with time resolution of less than 1 min, and improvement of a factor of 4--5 over previously reported methods using electron capture detection. Data

Jeffrey S. Gaffney; Nancy A. Marley; H. Donnan Steele; Paul J. Drayton; John M. Hubbe

1999-01-01

83

Determination of dissolved Fe(II) in seawater of the western North Pacific with luminol chemiluminescence method  

NASA Astrophysics Data System (ADS)

Determination of dissolved Fe(II) in seawater of the western North Pacific with luminol chemiluminescence method Hajime Obata, Akira Mase, Toshitaka Gamo (Atmosphere and Ocean Research Institute, University of Tokyo, Japan), Jun Nishioka (Institute of Low Temperature Science, Hokkaido University, Japan), Shigenobu Takeda (Faculty of Fisheries, Nagasaki University, Japan) Speciation of iron in the ocean is now important topics because the bioavailability of iron depends on its chemical form in seawater. However, marine biogeochemical process of Fe(II) has not been fully investigated. In this study, we determined Fe(II) in seawaters using the luminol chemiluminescence method after acidifying the samples to pH 6(Hansard and Landing, 2009). The same samples collected in the western North Pacific were analyzed by the flow chemiluminescence methods with acidification to pH 6 and without acidification. The results with both methods were almost identical. Time variation of Fe(II) in seawater after acidifying the samples to pH 6 were examined in the western North Pacific and the Bering Sea. Within 10 minutes, variations of Fe(II) were small in the open ocean waters, whereas Fe(II) concentrations increased rapidly in surface waters collected in the Bering Sea. The acidification method is not always applicable for seawater samples, especially in the marginal sea. Surface distributions of Fe(II) in the western subarctic North Pacific were investigated by using a continuous clean sampling system for surface waters. The Fe(II) concentrations ranged from <9 to 42 pM, which were lower than those in previous studies (Roy et al., 2008). The variation of Fe(II) probably reflects the photoreduction process of Fe(III), slow oxidation of Fe(II) and differences of Fe(II) concentrations among water masses. In this study, we also examined the oxidation process of Fe(II) in seawater of the western North Pacific and the Bering Sea at some temperatures. The oxidation rates were slower in the Bering Sea than those in the western North Pacific, implying that the oxidation rates were controlled not only by water temperature but also by organic compounds, such as humic substances.

Obata, H.; Mase, A.; Gamo, T.; Nishioka, J.; Takeda, S.

2010-12-01

84

Flow injection determination of cationic surfactants by using N-bromosuccinimide and N-chlorosuccinimide as new oxidizing agents for luminol chemiluminescence  

Microsoft Academic Search

Two highly sensitive chemiluminescence (CL) systems are described. The method is based on the CL generated during the oxidation of luminol by N-bromosuccinimide (NBS) and N-chlorosuccinimide (NCS) in alkaline medium. The emission intensity is reduced by the presence of some surfactants at concentrations lower than critical micelle concentration (cmc).A new, simple, rapid and selective flow injection CL method for the

Afsaneh Safavi; Mohammad Ali Karimi

2002-01-01

85

CoFe2O4 nanoparticles as oxidase mimic-mediated chemiluminescence of aqueous luminol for sulfite in white wines.  

PubMed

Recently, the intrinsic enzyme-like activity of nanoparticles (NPs) has become a growing area of interest. However, the analytical applications of the NP-based enzyme mimetic are mainly concentrated on their peroxidase-like activity; no attempts have been made to investigate the analytical applications based on the oxidase mimic activities of NPs. For the first time, we report that CoFe(2)O(4) NPs were found to possess intrinsic oxidase-like activity and could catalyze luminol oxidation by dissolved oxygen to produce intensified chemiluminescence (CL). The effect of sulfite on CoFe(2)O(4) NP oxidase mimic-mediated CL of aqueous luminol was investigated. It is very interesting that when adding sulfite to the luminol-CoFe(2)O(4) system, the role of sulfite in the luminol-CoFe(2)O(4) NP-sulfite system depends on its concentration. At a relatively low concentration level, sulfite presents an inhibition effect on the luminol-CoFe(2)O(4) NP system. However, it does have an enhancement effect at a higher concentration level. Investigations on the effect of the solution pH and luminol and CoFe(2)O(4) NP concentrations on the kinetic characteristics of the studied CL system in the presence of trace sulfite suggested that the enhancement and inhibition of the luminol-CoFe(2)O(4) NP-sulfite CL system also depended on the solution pH. It seems that the concentrations of luminol and CoFe(2)O(4) NPs did not influence the CL pathway. The possible mechanism of the luminol-CoFe(2)O(4) NP-sulfite CL system was also discussed. On this basis, a flow injection chemiluminescence method was established for the determination of trace sulfite in this study. Under the optimal conditions, the proposed system could respond down to 2.0 × 10(-8) M sulfite. The method has been applied to the determination of trace sulfite in white wine samples with satisfactory results. The results given by the proposed method are in good agreement with those given by the standard titration method. PMID:23289402

Zhang, Xiaodan; He, Shaohui; Chen, Zhaohui; Huang, Yuming

2013-01-16

86

Influence of polyclonal immunoglobulins on the polymorphonuclear leukocyte response to lipopolysaccharide of Salmonella enteritidis as measured with luminol-enhanced chemiluminescence.  

PubMed Central

In gram-negative sepsis, the activation of polymorphonuclear leukocytes (PMN) by lipopolysaccharide (LPS) and the resulting production of superoxide and other oxygen radicals may be an important cause of tissue damage. A suppression of the PMN response to LPS stimulation would be therapeutically beneficial. The aim of this study was to determine whether different polyclonal immunoglobulins (Igs; 5S-Ig, 7S-Ig, and 19S-Ig) influence the PMN response to LPS of Salmonella enteritidis in vitro. The respiratory burst activity of PMN was measured with luminol-enhanced chemiluminescence. After addition of a 5S-Ig solution containing F(ab')2 fragments of IgG and a 19S-Ig solution containing 12% polyclonal IgM, luminol-enhanced chemiluminescence was reduced by 27% (P < 0.05) and 46% (P < 0.005), respectively. However, after addition of a 7S-Ig solution containing polyclonal IgG, luminol-enhanced chemiluminescence was increased fourfold (P < 0.05). The results suggest that the influence of polyclonal Igs on PMN response to LPS stimulation is dependent on the Ig class, F(ab')2 fragments of IgG and IgM leading to LPS neutralization and IgG leading to the production of potentially toxic oxygen radicals.

Wagner, D R; Heinrich, D

1994-01-01

87

Demonstration that O2- is a Crucial Intermediate in the High Quantum Yield Luminescence of Luminol,  

National Technical Information Service (NTIS)

The chemiluminescence of luminol, due to its reaction with alkaline H2O2, is inhibited by superoxide dismutase or by hydroxyl radical scavengers. Hematin markedly enhances this H2O2- induced luminescence of luminol and lessens, but does not eliminate, the...

E. K. Miller I. Fridovich

1986-01-01

88

Fibre-optic biosensor for hypoxanthine and xanthine based on a chemiluminescence reaction.  

PubMed

Fibre-optic biosensors were constructed for determination of hypoxanthine and xanthine. Xanthine oxidase and peroxidase were immobilized on different preactivated membranes which were subsequently mounted onto the tip of a fibre-optic bundle. The H2O2 generated by the reaction of hypoxanthine and xanthine oxidase was measured by chemiluminescence (CL) detection using luminol and peroxidase. A linear calibration curve of the sensors in the range of 1-316 microM hypoxanthine and 3.1-316 microM xanthine, respectively, with a detection limit of 0.55 microM hypoxanthine was obtained. Recovery of hypoxanthine ranged between 91 and 102%. PMID:8060588

Hlavay, J; Haemmerli, S D; Guilbault, G G

1994-01-01

89

Calibrated Chemiluminescence Standards.  

National Technical Information Service (NTIS)

The aim of this project was to provide an absolute photometric calibration for an underwater photometer in use at the Naval Oceans Systems Center in San Diego. This was made by using as a reference light standard, the chemiluminescence reaction of luminol...

J. Lee I. B. C. Matheson J. R. Losee E. F. Zalewski

1982-01-01

90

A novel chemiluminescence assay of organophosphorous pesticide quinalphos residue in vegetable with luminol detection  

Microsoft Academic Search

BACKGROUND: Organophosphorous pesticides are the most popular pesticides used in agriculture. As acetylcholinesterase inhibitors, organophosphorous pesticides are toxic organic chemicals. The control and detection of organophosphorous pesticide residue in food, water, and environment therefore plays a very important role in maintaining physical health. A sensitive, rapid, simple chemiluminescence(CL) method has been developed for the determination of quinalphos based on the

Haoyu Hu; Xiaoyu Liu; Feng Jiang; Xin Yao; Xiaocheng Cui

2010-01-01

91

Chemiluminescence determination of moxifloxacin in pharmaceutical and biological samples based on its enhancing effect of the luminol-ferricyanide system using a microfluidic chip.  

PubMed

A sensitive determination of a synthetic fluoroquinolone antibacterial agent, moxifloxacin (MOX), by an enhanced chemiluminescence (CL) method using a microfluidic chip is described. The microfluidic chip was fabricated by a soft-lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three-inlet microchannels for introducing the sample, chemiluminescent reagent and oxidant, and a 500?µm wide, 250?µm deep and 82?mm long microchannel. An enhanced CL system, luminol-ferricyanide, was adopted to analyze the MOX concentration in a sample solution. CL light was emitted continuously after mixing luminol and ferricyanide in the presence of MOX on the PDMS microfluidic chip. The amount of MOX in the luminol-ferricyanide system influenced the intensity of the CL light. The linear range of MOX concentration was 0.14-55.0?ng/mL with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.06 and 0.2?ng/mL respectively. The presented method afforded good reproducibility, with a relative standard deviation (RSD) of 1.05% for 10?ng/mL of MOX, and has been successfully applied for the determination of MOX in pharmaceutical and biological samples. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23723140

Suh, Yeoun Suk; Kamruzzaman, Mohammad; Alam, Al-Mahmnur; Lee, Sang Hak; Kim, Young Ho; Kim, Gyu-Man; Dang, Trung Dung

2013-05-30

92

Alterations in luminol-enhanced chemiluminescence from nondiluted whole blood in the course of low-level laser therapy of angina pectoris patients  

NASA Astrophysics Data System (ADS)

Addition of Luminol to nondiluted blood of healthy donors results in a short and weak increase of chemiluminescence (CL) from it. Contrary to that in 25 cases of stable angina pectoris the intensity of CL from blood of patients sharply increased upon addition of luminol exceeding that form healthy donors' blood 10-100-fold. 24 hours after the 3D intravenous low-level treatment CL burst in patients' blood in the presence of Luminol was in general significantly lower than before the beginning of the treatment. After the 7th treatment the pattern of CL kinetics was in most cases similar to that of healthy donors' blood. However, after the 10th treatment intensity of Luminol-enhanced CL usually increased and for blood of some patients even exceeded its values obtained before the treatment. Some correlation CL from nondiluted blood with neutrophil activity studied by NTB-test and plasma viscosity of same blood was noted. Using highly sensitive single photon counters it is possible to reveal abnormal levels of CL from no more than 0.1-0.2 ml of blood within 3-5 min.

Voeikov, Vladimir L.; Novikov, Cyrill N.; Siuch, Natalia I.

1997-05-01

93

Aircraft measurements of nitrogen dioxide and peroxyacyl nitrates using luminol chemiluminescence with fast capillary gas chromatography  

SciTech Connect

Peroxyacyl nitrates (PANs) and nitrogen dioxide (NO{sub 2}) are important trace gas species associated with photochemical air pollution. The PANs are in thermal equilibrium with the peroxyacetyl radical and NO{sub 2}. Because PANs are trapped peroxy radicals, they are an important indicator species of the photochemical age of an air parcel, as well as being a means of long-range transporting of NO{sub 2}, leading to the formation of regional ozone and other oxidants. Typically, PANs are measured by using a gas chromatograph with electron-capture detection (ECD). Once automated, this method has been shown to be reliable and quite sensitive, allowing the levels of PANs to be measured at low parts per trillion in the troposphere. Unfortunately, a number of other atmospheric gases also have strong ECD signals or act as inferences and limit the speed in which the analysis can be completed. Currently, the shortest analysis time for PAN is approx. 5 minutes with ECD. The authors recent examined the luminol detection of NO{sub 2} and PANs using gas capillary chromatography for rapid monitoring of these important trace gases. Analysis of the PANs (PAN, PPN, and PBN) and NO{sub 2} in one minute has been demonstrated in laboratory studies by using this approach. Reported here are modifications of this instrument for aircraft operation and preliminary results from test flights taken near Pasco, Washington in August of 1997.

Gaffney, J.S.; Marley, N.A. [Argonne National Lab., IL (United States). Environmental Research Div.; Drayton, P.J. [Univ. of Chicago, IL (United States). Dept. of Geophysical Sciences

1997-09-01

94

Aircraft measurements of nitrogen dioxide and peroxyacetyl nitrates using luminol chemiluminescence with fast capillary gas chromatography  

SciTech Connect

Fast capillary gas chromatography with luminol detection has been used to make airborne measurements of nitrogen dioxide (NO{sub 2}) and peroxyacetyl nitrate (PAN). The analysis system allows for the simultaneous measurement of NO{sub 2} and peroxyacyl nitrates (PANs) with time resolution of less than 1 min, and improvement of a factor of 4--5 over previously reported methods using electron capture detection. Data presented were taken near Pasco, Washington, in August 1997, during a test flight onboard the US Department of Energy G-1 aircraft. The authors report measurements of NO{sub 2} in the boundary layer in a paper mill plume and a plume from a grass fire, in addition to analyses for free tropospheric NO{sub 2} and PAN. Ratios of PAN/NO{sub 2} were observed to increase with altitude (decreasing temperature) and to reach values of 2--4 above the boundary layer, consistent with the thermal equilibrium of the peroxyacetyl radical and NO{sub 2} and PAN. Estimates for the peroxyacetyl radical in the continental free troposphere, calculated from this equilibrium, were found to be in the range of 10{sup 4}--10{sup 5} molecules per cubic centimeter. These results demonstrate the application of this approach for airborne measurements of NO{sub 2} and PAN in a wide range of field study scenarios.

Gaffney, J.S.; Marley, N.A.; Steele, H.D.; Drayton, P.J.; Hubbe, J.M.

1999-10-01

95

Cobalt determination in natural waters using cation-exchange liquid chromatography with luminol chemiluminescence detection  

SciTech Connect

A method has been developed for the analysis of cobalt in natural waters by cation-exchange liquid chromatography using chemiluminescence detection. Cobalt can be determined directly in freshwater samples on 500-..mu..L samples with a detection limit of 20 pmol/kg; larger samples provide proportionately lower detection limits. Seawater samples can be analyzed on 100-mL samples following APDC solvent extraction; the detection limit of this method is 5 pmol/kg. The precision of the method is +/- 5%. The method should also be applicable to the analysis of V, Cu, and Fe in natural waters. Equipment is low in cost and transportable and can be used in the field.

Boyle, E.A.; Handy, B.; van Geen, A.

1987-06-01

96

Studies on the mechanism of the peroxyoxalate chemiluminescence reaction  

Microsoft Academic Search

Further consideration has been given to the reaction pathway of a model peroxyoxalate chemiluminescence system. Again utilising doubly labelled oxalyl chloride and anhydrous hydrogen peroxide, 2D EXSY 13C nuclear magnetic resonance (NMR) spectroscopy experiments allowed for the characterisation of unknown products and key intermediate species on the dark side of the peroxyoxalate chemiluminescence reaction. Exchange spectroscopy afforded elucidation of a

Sarah A. Tonkin; Richard Bos; Gail A. Dyson; Kieran F. Lim; Richard A. Russell; Simon P. Watson; Christopher M. Hindson; Neil W. Barnett

2008-01-01

97

Transition Metal Complexes as Mediators in Photochemical and Chemiluminescence Reactions  

Microsoft Academic Search

Transition metal complexes can play the role of photosensitizes in light driven electron transfer reactions and the role of chemiluminescence inducers in exoergonic electron transfer reactions. A unifying view of these processes is given and their relevance concerning the interconversion of light, chemical energy and electrical energy is emphasized. Ru(bpy) is presently the most used photosensitizer and chemiluminescence inducer. Several

Vincenzo Balzani; Fabrizio Bolletta

1983-01-01

98

Characterization of the Luminol-Amplified Light Generating Reaction Induced in Human Monocytes  

Microsoft Academic Search

Increased productIon of oxidative metabolites following interaction between mononu- clear phagocytes and soluble stimuli can be measured as luminol-amplified chemilumi- nescence (CL). The effects of superoxide dlsmutase (SOD), catalase, and azide on the monocyte CL response were investigated. Azide, a myeloperoxidase (MPO) inhibitor, reduced the CL reaction by more than 80%, which indicates that the CL reaction is dependent on

Agneta Johansson; Claes Dahigren

99

Luminol chemiluminescence in unbuffered solutions with a cobalt(II)-ethanolamine complex immobilized on resin as catalyst and its application to analysis.  

PubMed

Using a heterogeneous catalyst, Co(II)-ethanolamine complex sorbed on Dowex-50W resin, the chemiluminescence (CL) of luminol in unbuffered or weakly acidic solution was studied in the presence of H2O2. The maximum luminol CL wavelength at pH 5.7 was 448 nm, 23 nm longer than that in a basic solution (pH 10.5). Three different ligands, mono-, di-, and triethanolamine, and six transition metal ions, Co(II), Cu(II), Ni(II), Mn-(II), Fe(II), and Fe(III) were compared by CL measurements. The CL intensity decreased in the order mono- > di- > triethanolamine and Co(II) > Cu(II) > Ni(II) > Fe-(III) > Mn(II) > Fe(II). This heterogeneous CL system was developed as H2O2 and glucose flow-through sensors. Detection limits (S/N = 3) of H2O2 and glucose using Dowex-50W-X4-Co(II)-monoethanolamine as catalyst are 1 x 10(-7) M and 1 x 10(-6) M, respectively. On the basis of the studies of the CL, fluorescence, UV-vis and ESCA spectra and the effect of dissolved oxygen in luminol solution, a mechanism for CL emission in unbuffered solution was considered as the formation of a superoxide radical ion during the decomposition of H2O2 catalyzed by the Co(II)-ethanolamine immobilized resin. Then the superoxide radical ion acted on luminol and the CL was emitted. The applications of the proposed method to determine H2O2 in rainwater without any special pretreatment and glucose in human urine and orange juice samples give satisfactory results. PMID:11721898

Lin, J M; Shan, X; Hanaoka, S; Yamada, M

2001-11-01

100

Determination of hydrogen peroxide concentrations by flow injection analysis based on the enhanced chemiluminescent reaction using peroxidase  

SciTech Connect

The technique of flow injection analysis was employed in the determination of hydrogen peroxide. The method was based on the chemiluminescence reaction of luminol with H{sub 2}O{sub 2} which is catalyzed by horseradish peroxidase and enhanced by p-iodophenol. Hydrogen peroxide was linearly detected in the range 10{sup {minus}6}M-10{sup {minus}4}M by measuring the maximum intensity of light emitted. The detection limit is about 1 10{sup {minus}6}M hydrogen peroxide. Transition metal cations at millimolar concentrations do not have any interference on the determination of hydrogen peroxide by FIA based on the enhanced chemiluminescent reaction. This technique is relatively rapid and simple, and permits measurement of up to 80 samples/hr using generally available equipment.

Eremin, S.A.; Vlasenko, S.B.; Osipov, A.P.; Eremina, I.D.; Egerov, A.M. (M.V. Lomonosov State Univ., Moscow (USSR))

1989-01-01

101

Comparative study of ?-glucan induced respiratory burst measured by nitroblue tetrazolium assay and real-time luminol-enhanced chemiluminescence assay in common carp (Cyprinus carpio L.).  

PubMed

The respiratory burst is an important feature of the immune system. The increase in cellular oxygen uptake that marks the initiation of the respiratory burst is followed by the production of reactive oxygen species (ROS) such as superoxide anion and hydrogen peroxide which plays a role in the clearance of pathogens and tissue regeneration processes. Therefore, the respiratory burst and associated ROS constitute important indicators of fish health status. This paper compares two methods for quantitation of ROS produced during the respiratory burst in common carp: the widely used, single-point measurement based on the intracellular reduction of nitroblue tetrazolium (NBT) and a real-time luminol-enhanced assay based on the detection of native chemiluminescence. Both assays allowed for detection of dose-dependent changes in magnitude of the respiratory burst response induced by ?-glucans in head kidney cells of carp. However, whereas the NBT assay was shown to detect the production of only superoxide anions, the real-time luminol-enhanced assay could detect the production of both superoxide anions and hydrogen peroxide. Only the chemiluminescence assay could reliably record the production of ROS on a real-time scale at frequent and continual time intervals for time course experiments, providing more detailed information on the respiratory burst response. The real-time chemiluminescence assay was used to measure respiratory burst activity in macrophage and neutrophilic granulocyte-enriched head kidney cell fractions and total head kidney cell suspensions and proved to be a fast, reliable, automated multiwell microplate assay to quantitate fish health status modulated by ?-glucans. PMID:23454430

Vera-Jimenez, N I; Pietretti, D; Wiegertjes, G F; Nielsen, M E

2013-02-27

102

Luminol-based nitrogen dioxide detector  

Microsoft Academic Search

An instrument for the continuous detection of NOâ in the sub-part-per-billion range is described. The instrument is based upon the chemiluminescent reaction between NOâ in air and luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) in alkaline solution. The present detector exhibits a 2-Hz response speed to changes of +\\/-20 ppB and a field detection limit of 30 parts per trillion. The instrumental technique has been

Gregory J. Wendel; Donald H. Stedman; Christopher A. Cantrell; Lenore. Damrauer

1983-01-01

103

Organ chemiluminescence: noninvasive assay for oxidative radical reactions.  

PubMed Central

In situ and perfused rat livers showed a spontaneous chemiluminescence of 7-12 counts/sec . cm2 (corresponding to 7-12 x 10(3) photons/sec . cm2); chemiluminescence was increased up to 30 times by infusion of exogenous hydroperoxides. The chemiluminescence of the perfused liver was oxygen dependent. Ethyl, t-butyl, and cumene hydroperoxides were almost equally effective in inducing light emission in the perfused liver. Glutathione release and chemiluminescence showed a parallel increase upon hydroperoxide supply to the perfused liver. A partial spectral analysis of the chemiluminescence of the perfused liver showed a predominance of red-light-emitting species, presumably arising from the singlet oxygen dimol-emission peaks. Many side reactions derived from the complex free radical sequence of lipid peroxidation could afford the chemistry leading to light emission, which represents only about 10(-14) of the utilization of peroxide.

Boveris, A; Cadenas, E; Reiter, R; Filipkowski, M; Nakase, Y; Chance, B

1980-01-01

104

Flow-injection chemiluminescence determinations for human blood lead using controlled reagent release technology  

Microsoft Academic Search

A flow-through CL method for the determination of lead combined with controlled-reagent-release technology has been developed.\\u000a Chemiluminescence (CL) reagents luminol and potassium permanganate were immobilized on anion exchange resin by electrostatic\\u000a interaction. Lead ion was determined by its enhancing effect on the CL reaction between luminol and potassium permanganate.\\u000a Both luminol and potassium permanganate were eluted from the anion exchange

Peng Qu; Shan Cheng Yan; Hua Lu; Zu Hong Lu

2008-01-01

105

Selective Analysis of Chlorine (Hypochlorous Acid) and Chlorine Dioxide Using Chemiluminescence  

Microsoft Academic Search

Using chemiluminescence produced by reactions with luminol and hydrogen peroxide a method for simultaneous and selective analysis of chlorine (hypochlorous acid) and chlorine dioxide has been developed. A continuous flow technique is used and the light emitted at two sites along the liquid flow is recorded. Selectivity is based on the difference in the rates at which the chemiluminescence, produced

Ulf Isacsson; Gunnar Wettermark

1978-01-01

106

Hemin as a Catalyst for Chemiluminescence.  

National Technical Information Service (NTIS)

The chemiluminescence produced by the reaction of luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) in a basic solution in the presence of hydrogen peroxide and catalyzed by protohemin (protoporphyrin IX iron III chloride) has been well studied. The actu...

T. P. Vasileff G. Svarnas H. A. Neufeld L. Spero

1973-01-01

107

Chemiluminescent Reaction of Tellurium with Fluorine.  

National Technical Information Service (NTIS)

Tellurium vapor reacts with gaseous fluorine to produce a chemiluminescent flame. The emission in the visible and near infrared part of the spectrum of the flame has been previously attributed to the A2 psi - X 2 psi - X system of TeF. Excited TeF produce...

H. W. Moody J. A. Menapace C. J. Dymek M. L. Scott

1982-01-01

108

p-Iodophenol-enhanced luminol chemiluminescent assay applied to discrimination between acute lymphoblastic and minimally differentiated acute myeloid (FAB-M0) or acute megakaryoblastic (FAB-M7) leukemias  

Microsoft Academic Search

Introduction: In this report, we propose the application of the p-iodophenol-enhanced luminol chemiluminescent technique to the determination of peroxidase (myeloperoxidase and\\/or platelet peroxidase) activity in blasts of minimally differentiated acute myeloblastic leukemia (AML-M0) and acute megakaryoblastic leukemia (AML-M7). Methods: The frozen blast cells from 29 patients were thawed and submitted to the optimized protocol. Results: All cases of AML-M7 and

Miriane da Costa; Valdecir Farias Ximenes; Iguatemy Lourenço Brunetti; Roberto Passetto Falcão; Luiz Marcos da Fonseca

2004-01-01

109

Summary of activities and accomplishments. Volume IV. A proposal to develop a method for the detection of HE employing chemiluminescence reactions. Final progress report  

SciTech Connect

This is the final and fourth quarter report for the study of high explosive (HE) detection by coupling the chemistry of HE with that of luminol reaction, a well-known chemiluminescence (CL) reaction. Our accomplishments include: success in coupling HE and CL chemistry reliably; the capability to use a micellized solvent to concentrate HE; and the basis for design instrumentation that may exhibit better sensitivity and lower levels of detection than that exhibited by the laboratory apparatus used for this study. On the basis of these results we are prepared to recommend further study.

Neary, M.P.

1981-01-01

110

A new chemiluminescence method for the determination of nickel ion  

Microsoft Academic Search

A new chemiluminescence (CL) phenomenon described as the second-chemiluminescence (SCL) was observed and a strong CL signal was detected, when Ni(II) ion was injected into the mixture after the end of the reaction of potassium permanganate with alkaline luminol. The possible CL mechanism is proposed based on the kinetic curve of the CL reaction, CL spectra, UV–vis spectra and some

Li Na Li; Nian Bing Li; Hong Qun Luo

2006-01-01

111

Development and optimization of an analytical method for the determination of Sudan dyes in hot chilli pepper by high-performance liquid chromatography with on-line electrogenerated BrO- -luminol chemiluminescence detection.  

PubMed

The determination of four Sudan dyes by means of high-performance liquid chromatography (HPLC) with chemiluminescence (CL) detection was proposed. The method was based on the enhancement effect of Sudan dyes on the chemiluminescence reaction between luminol and BrO-, which was on-line electrogenerated by constant current electrolysis. The separation was carried out on Nucleosil RP-C18 column (250 mm x 4.6 mm i.d., 5 microm, pore size, 100 A) at 35 degrees C. The mobile phase consisted of a V (methanol): V (0.2% aqueous formic acid) = 90:10 solution. At a flow-rate of 1.0 ml min(-1), the total run time was 25 min. The effects of several parameters on the HPLC resolution and CL emission were studied systematically. For the four Sudan dyes, the limits of detection (LOD) at a signal-to-noise of 3 ranged from 4 to 8 microg kg(-1) and the limits of quantification (LOQ) at a signal-to-noise of 10 ranged from 13 to 27 microg kg(-1). The relative standard deviations (RSD) of intra-and inter-day precision were below 4.4%. The average recoveries for all four Sudan dyes (spiked at the levels of 1.0 and 1.5 mg kg(-1)) in chilli tomato sauce and hot chilli pepper ranged from 94% to 105%, and the relative standard deviations of the quantitative results were from 2.5 to 4.2%. The proposed method had been successfully applied to the determination of four Sudan dyes in hot chilli products. PMID:16919285

Zhang, Yantu; Zhang, Zhujun; Sun, Yonghua

2006-08-17

112

Fluctuation dynamics of chemiluminescence in the maillard reaction  

Microsoft Academic Search

The general problems of information deriving from the oscillation kinetics of Belousov-Zhabotinsky reactions were discussed.\\u000a The discussion was organized around a particular example of the complex fluctuation dynamics of the Maillard reaction, i.e.,\\u000a a multistage reaction between a carbonylcontaining compound and the nucleophilic amino group of an amino-containing compound\\u000a at different temperatures, as visualized by chemiluminescence. Flicker-noise spectroscopy served as

S. G. Lakeev; V. L. Voeikov; S. F. Timashev

2011-01-01

113

Porphyrin-induced photogeneration of hydrogen peroxide determined using the luminol chemiluminescence method in aqueous solution: A structure-activity relationship study related to the aggregation of porphyrin.  

PubMed

A luminol chemiluminescence method was used to evaluate the porphyrin-induced photogeneration of hydrogen peroxide (H2O2). This method enabled us to detect H202 in the presence of a high concentration of porphyrin, which was not possible using conventional colorimetry. The limit of detection was about 1 microM. We compared the ability to generate H2O2, using uroporphyrin (UP), hexacarboxylporphyrin (HCP), coproporphyrin (CP), hematoporphyrin (HP), mesoporphyrin (MP), and protoporphyrin (PP). The amount of H2O2 photoproduced was strongly related to the state of the porphyrin in the aqueous solution. UP and HCP, which existed predominantly in a monomeric form, had a good ability to produce H2O2. HP and MP, existing as dimers, showed weak activity. CP, forming a mixture of monomer and dimer, had a moderate ability to produce H2O2. PP, which was highly aggregated, had a good ability. These results demonstrated that the efficiency of porphyrins to produce H2O2 was strongly dependent on their aggregated form, and the dimer suppressed the production of H2O2. PMID:16512418

Komagoe, Keiko; Katsu, Takashi

2006-02-01

114

Analytical assessment of the oscillating chemical reactions by use chemiluminescence detection.  

PubMed

This paper introduces the chemiluminescence (CL) detection in oscillating reaction-based determinations using the analyte pulse perturbation technique, a straightforward and expeditious approach to deriving quantitative analytical information from oscillating chemical reactions. The behavior of the H(2)O(2)-KSCN-CuSO(4)-NaOH oscillating system in the presence of luminol was examined by using the proposed detection method and the classical potentiometric technique. Some analytical and practical aspects of both detection systems are discussed. A new analytical method for the determination of vitamin B(6) based on the sequential perturbation produced by different amounts of this substance on the oscillating chemical system was also developed in order to assess the potential of CL detection for routine analyses. The calibration curve thus obtained was linear over the range 0.5-2.0 mumol of vitamin B(6), and the precision and throughput were also quite good (3.04% as RSD and nine samples h(-1), respectively). The proposed method was validated by determining the vitamin in pharmaceutical preparations. PMID:18966884

Jiménez-Prieto, R; Silva, M; Pérez-Bendito, D

1997-08-01

115

Generation of an oscillating chemical reaction in a heterogeneous system by the oxidation of an alkaline solution of luminol with sodium(vi) ferrate in the presence of copper ions  

Microsoft Academic Search

Chemiluminescence accompanying the oxidation of 3-aminophthalhydrazide (luminol) with sodium ferrate (vi) in an alkaline solution is studied. The powerful initial light flash flaring up at the moment of mixing the reactants is followed by a continuous low-intensity luminescence in the heterogeneous system formed. The addition of Cu2+ ions during this period results in a new chemiluminescence flash with an oscillating

D. Yu. Stoupine; Y. K. Gusev; D. V. Lachkova

2001-01-01

116

Luminol-based nitrogen dioxide detector  

SciTech Connect

An instrument for the continuous detection of NO/sub 2/ in the sub-part-per-billion range is described. The instrument is based upon the chemiluminescent reaction between NO/sub 2/ in air and luminol (5-amino-2,3-dihydro-1,4-phthalazinedione) in alkaline solution. The present detector exhibits a 2-Hz response speed to changes of +/-20 ppB and a field detection limit of 30 parts per trillion. The instrumental technique has been expanded to measure NO by the catalytic oxidation of NO to NO/sub 2/ using CrO/sub 3/ on silica gel as the oxidizing agent; however, at low ambient NO concentrations some drift in the NO zero is observed. Interference from ambient O/sub 3/ is elimated by modification of the inlet system and luminol solution.

Wendel, G.J.; Stedman, D.H.; Cantrell, C.A.; Damrauer, L.

1983-05-01

117

Phenomenon of “chaos” in an oscillatory chemiluminescent reaction  

Microsoft Academic Search

sof the chemiluminescence intensity l(t) in the [S--03--UO~+--H2SO4 (cone.)] system in the presence of Cr~O~- ions was given in [i0]. The CL in this system is caused by the oxidation of sulfur by ozone and the bichromate ion. The energy of the oxidation reaction is transferred to the UO~ + uranyl ion, which is a photon emitter. Chaotic changes in

V. A. Antipin; V. P. Kazakov; G. S. Parshin

1984-01-01

118

Evaluation of the catalytic decomposition of H2O2 through use of organo-metallic complexes--a potential link to the luminol presumptive blood test.  

PubMed

Forensic scientists use several presumptive tests to detect latent blood stains at crime scenes; one of the most recognizable being the luminol reagent. Luminol, under basic conditions, reacts with an oxidizing species which, with the help of a transition metal catalyst facilitates a luminescent response. The typical oxidizing species used in the luminol reaction is hydrogen peroxide (H(2)O(2)). While the luminol reaction has been studied since its inception, the mechanistic pathway is still an area of great debate. Previous work suggests that the luminol reaction with latent blood stains possesses a correlation to the Fenton-Decomposition reaction mechanism, which decomposes H(2)O(2) into the strongly oxidizing hydroxyl radical (*OH) species. This work seeks to understand the luminol reaction on a mechanistic level and to determine if a synergy exists between the chemiluminescence observed in the reaction and the production of the hydroxyl radical via Fenton-like processes. Results indicate that organo-metallic complexes produce hydroxyl radicals at different rates and different concentrations. These findings appear to be related to structural differences in the organo-metallic complex, which conform to the 18 electron rule or are one electron rich/deficient. Furthermore, the production of *OH is controlled by the chemical environment which governs complex stability at high pH conditions, reflective of the luminol process. Model hemoglobin systems reveal a strong correlation between the rate of *OH production via the Fenton-like pathway and maximum chemiluminescent intensity. PMID:22227152

Soderquist, Thomas J; Chesniak, Olivia M; Witt, Matthew R; Paramo, Alan; Keeling, Victoria A; Keleher, Jason J

2012-01-05

119

High-performance liquid chromatographic separation and quantification of citric, lactic, malic, oxalic and tartaric acids using a post-column photochemical reaction and chemiluminescence detection  

Microsoft Academic Search

An HPLC method was developed for the determination of citric, lactic, malic, oxalic and tartaric acids by chemiluminescent detection following online irradiation with visible light. The organic acids were irradiated with visible light in the presence of Fe3+ and UO22+ to generate Fe2+, which was determined by measuring the chemiluminescence intensity in a luminol system in the absence of added

Tomás Pérez-Ruiz; Carmen Mart??nez-Lozano; Virginia Tomás; Jesús Mart??n

2004-01-01

120

Selective determination of amino acids using flow injection analysis coupled with chemiluminescence detection  

Microsoft Academic Search

The determination of the amino acids proline, histidine, tyrosine, arginine, phenylalanine and tryptophan using flow injection analysis (FIA) with chemiluminescence detection is described. Proline was the only amino acid to exhibit chemiluminescence with the tris(2,2-bipyridyl)ruthenium(III) reaction at pH 10. While, histidine was found to selectively enhance the reaction of luminol with Mn(II) salts in a basic medium. Acidic potassium permanganate

Jason W Costin; Paul S Francis; Simon W Lewis

2003-01-01

121

A Sensitive Photoinduced Chemiluminescence Method for the Determination of Riboflavin with Flow Injection Analysis  

Microsoft Academic Search

A sensitive chemiluminescence (CL) induced by light for the determination of riboflavin with flow injection analysis is developed. Riboflavin shows a strong enhancement effect on the CL reaction of luminol oxidized by periodate after the photochemical reaction of riboflavin in alkaline solution, and the enhancing effect of riboflavin disappears under dark conditions. Oxygen can further improve the enhancing effect of

Guo-Fang Zhang; Hong-Yuan Chen

2000-01-01

122

Fabrication of gold nanoparticles using luminol as a reductive and protective reagent  

Microsoft Academic Search

An easy and simple reaction is employed to synthesize ligand-protected gold nanoparticles. In this reaction, luminol is used\\u000a as a reductive and protective reagent to prepare gold nanoparticles in aqueous solution. It includes two steps: chemiluminescence\\u000a and formation of gold nanoparticles. The formation processes were monitored by resonance light scattering spectroscopy, and\\u000a the results were observed by transmission electron microscopy

Baoqiang Lv; Xiaodong Su; Yang Li; Yi Li; Jianfei Mao; Dan Xiao

2008-01-01

123

Fast Detection of Explosives Vapours and Particles by Chemiluminescence Technique  

Microsoft Academic Search

A chemiluminescence (CL) apparatus has been devised for the detection of nitro-containing explosives based on its reaction\\u000a with luminol. This reaction is highly selective for NO2 under certain conditions, so that other nitrogen-containing compounds such as ammonia, organic nitrite, organic nitrate,\\u000a NO and hydrocarbons do not interfere. The detection system uses a gas-liquid reaction leading to light emission with a

Dao Hinh Nguyen; Shirley Locquiao; Phuong Huynh; Qiaoling Zhong; Wen He; David Christensen; Lin Zhang; Bill Bilkhu

124

Application of chemiluminescent indicators in EDTA titrations.  

PubMed

The chemiluminescent indicators lucigenine and luminol are readily applicable to end-point detection in the EDTA titration of cadmium, zinc and nickel ions. Back-titration with copper(II) gives reproducible results. The end-point is indicated by the disappearance of the green chemiluminescence of lucigenine, or by the appearance of the bluish luminescence of luminol. PMID:18960856

Erdey, L; Weber, O; Buzás, I

1970-12-01

125

[Chemiluminescence and phagocytic reactions of human nuclear leukocytes incubated with Streptococcus sanguis].  

PubMed

This experiment was designed to determine the role of indigenous organisms in gingival inflammation of the oral cavity, The test bacteria consisted of 3 strains of Streptococcus sanguis isolated from plaque at the gingival margin and grown in sure culture. These strains were subsequently designated as A, B and C. Peripheral nuclear leukocytes were isolated from 5 test subjects with clinically healthy gingiva. Luminol-dependent chemiluminescence and phagocytic functions of the 3 strains were measured, with the following results obtained. The mean peak value of luminol-dependent chemiluminescence following stimulation for strain A was 80.8 in the presence of serum, 38.8 in the presence of inactivated serum, and 20.5 in the absence of serum. For strain B it was 82.3 in the presence of serum, 6.4 in the presence of inactivated serum, and 2.5 in the absence of serum. For strain C it was 68.1 in the presence of serum, 65.3 in the presence of inactivated serum, and 8.3 in the absence of serum. In the absence of plasma strains A and B showed slight phagocytosis. In strain C, however, no phagocytosis was noted. In the presence of inactivated serum phagocytosis was increased strains A and B, but no phagocytosis was noted in strain C. In the presence of blood. phagocytosis was increased in strains A and B, but no phagocytosis was noted in strain C. PMID:2637295

Kobayashi, N; Kamoi, K

1989-06-01

126

Fluorimetry Studies of Oscillating Chemilumnescence in the Luminol= H 2 O 2 = KSCN= CuSO 4 = TMAOH System  

Microsoft Academic Search

Oscillating chemical reactions are complex systems, involving a large number of chemical species. In oscillating chemical reaction, some species, usually a reaction intermediate, exhibit fluctuation in its concentration. In this report, visible oscillating chemiluminescence produced by the addition of luminol (3-aminophthalhydrazide) to the oscillating system of H2O2=KSCN=CuSO4=TMAOH was investigated using spectrofluorimetry method. The effects of ingredient concentration of the oscillating

Abdolraouf Samadi-Maybodi; Niloofar Naseri; M. J. Chaichi

2005-01-01

127

Organ Chemiluminescence: Noninvasive Assay for Oxidative Radical Reactions  

Microsoft Academic Search

In situ and perfused rat livers showed a spontaneous chemiluminescence of 7-12 counts \\/ sec\\\\cdot cm2 (corresponding to 7-12× 103 photons \\/ sec\\\\cdot cm2); chemiluminescence was increased up to 30 times by infusion of exogenous hydroperoxides. The chemiluminescence of the perfused liver was oxygen dependent. Ethyl, t-butyl, and cumene hydroperoxides were almost equally effective in inducing light emission in the

Alberto Boveris; Enrique Cadenas; Rudolf Reiter; Mark Filipkowski; Yuzo Nakase; Britton Chance

1980-01-01

128

Inhibition by nonsteroidal antiinflammatory drugs of luminol-dependent human-granulocyte chemiluminescence and /sup 3/H FMLP binding. Effect of sulindac sulfide, indomethacin metabolite, and optical enantiomers (+) and (-) MK830  

SciTech Connect

A system is described to evaluate for nonsteroidal antiinflammatory drugs by means of luminol-dependent human-granulocyte chemiluminescence (CL) is described. The CL is produced using either opsonized zymosan (yeast cells) or the soluble chemotactic peptide f-Met-Leu-Phe as the perturbant of the granulocyte membrane. Using either system, the following drug effects 2 x 10(-5) M were noted: only sulindac sulfide, and not sulindac sulfone or sulindac, displayed marked inhibition of chemiluminescence, following the in vivo data regarding inflammatory effects. The 5-OH indomethacin metabolite was likewise inactive as an inhibitor of CL mirroring in vivo effects. MK(+)410, MK(-)830 and MK835 all showed approximately 50% inhibition of CL, displaying deviation from in vivo data. MK(+)830 markedly stimulated CL, 4-6 times the control (without drug), which is clearly different from its enantiomer, MK(-)830. The reasons for this behavior are unclear. However, receptor binding studies with /sup 3/H FMLP were accomplished in the presence and absence of the various drugs at 2 x 10(-5) M that were effective inhibitors of chemiluminescence (CL). Indomethacin, MK(-)830 and MK(+)410 had equivalent percent control binding and percent control CL. Sulindac sulfide and MK(+)835 both had higher percent control binding than percent control CL, with MK(+)835 displaying apparent increased numbers of available receptors relative to control. MK(+)830, which produces large increases in CL, produced a minor effect on percent control binding. A direct relationship between binding and CL does not exist with each drug. Chemiluminescence is dependent on ion movement and oxidative metabolism and is a secondary event to agonist-receptor occupation.

Van Dyke, K.; Peden, D.; Van Dyke, C.; Jones, G.; Castranova, V.; Ma, J.

1982-03-01

129

The Luminescence of Luminol xi.  

National Technical Information Service (NTIS)

The luminol reaction may serve very well for the detection of blood spots and traces of blood on various objects in judicial-medical and criminological practice. There are various recipes for preparing the luminol reagent, among which that with sodium hyd...

K. Weber V. Mikulovic

1964-01-01

130

Infrared Chemiluminescence Studies of Ion-Molecule Reactions in a Flowing Afterglow.  

National Technical Information Service (NTIS)

A flowing afterglow apparatus for infrared chemiluminescence studies of ion-molecule reactions has been successfully constructed, made operational, and two key new experiments completed in the first year of this grant. Figure 1 illustrates the major compo...

S. R. Leone G. B. Ellison V. M. Bierbaum

1979-01-01

131

Fast and sensitive chemiluminescence assay of aminophylline in human serum using luminol-diperiodatoargentate(III) system catalyzed by coated iron nanoparticles  

NASA Astrophysics Data System (ADS)

The CL intensity of luminol-diperiodatoargentate(III) (DPA) system is strongly enhanced by addition of iron nanoparticles (FeNPs) covered with C12E4. On injection of aminophylline into luminol-DPA-FeNPs system, the CL intensity is significantly increased. On this basis, a sensitive CL assay was developed for determination of AmP in human serum. FeNPs could catalyze the oxidation rate of luminol in the present of oxygen. Also, the CL intensity of luminol-DPA-FeNPs system is significantly increased in the presence of aminophylline (AmP). Based on this ruling, a sensitive CL assay was developed for determination of AmP in human serum. The influences of analytical variables on the CL signal were studied and optimized. Under the optimum conditions in the present of FeNPs, the CL intensity is linearly increased with AmP concentration in the range of 1.0 × 10-8-2.0 × 10-6 mol L-1. The detection limit was 9.8 × 10-9 mol L-1 AmP and the relative standard deviation for ten parallel measurements of 8.0 × 10-7 mol L-1 AmP was also 4.8%. The proposed system was successfully applied to determine AmP in human serum samples.

Rezaei, B.; Ensafi, Ali A.; Zarei, L.

2012-05-01

132

Detection of Various a-Substituted Ketones via Chemiluminescence of 5-Amino-2-3-Dihydro-1,4-Phthalazinedione.  

National Technical Information Service (NTIS)

A number of alpha-substituted ketones and related compounds are capable of inducing the chemiluminescence of 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) with hydrogen peroxide in basic solution. The reaction apparently involves formation of interme...

H. W. Yurow S. Sass

1973-01-01

133

Deuteration effect in auto-oscillation chemiluminescence of the Belousov-Zhabotinskii reaction  

Microsoft Academic Search

It has been established that substitution of H2O by D2O in the auto-oscillation system of malonic acid+bromate+catalyst (Ce3+ 4+, Ru(bipy)32+, 3+) not only changes the parameters of oscillations recorded according to the chemiluminescence intensity, but can also lead to their complete disappearance. The step that is responsible for the D2O effect on chemiluminescent oscillation reactions is the deuteration of the

A. D. Karavaev; V. P. Kazakov; G. A. Tolstikov

1986-01-01

134

Analytical use of regenerative chemiluminescence in oscillatory reactions  

Microsoft Academic Search

The authors present a detailed study of the chemiluminescence of ruthenium tris-2,2'-dipyridy1 complex Ru(bipy)â² in the system potassium bromate-malonic acid-cerium (III, IV) sulfate. It is found that chemiluminescence of dipyridy1 ruthenium compounds in the BrOâ-reverse arrow CHâ(COOH)â-Ce (III) system enables the detection of the element at very low concentrations (down to about 2.10⁻¹° M). The dependence of the maximum intensity

A. D. Karavaev; V. P. Kazakov; N. L. Khokhlova; G. A. Tolstikov; V. V. Yakshin

1986-01-01

135

Studies on the mechanism of the peroxyoxalate chemiluminescence reaction  

Microsoft Academic Search

A simple model peroxyoxalate chemiluminescence system was monitored directly across a range of temperatures (from ?80 to +20°C) using 13C nuclear magnetic resonance spectroscopy. These experiments were made possible by the utilisation of 13C doubly labelled oxalyl chloride, which was reacted with anhydrous hydrogen peroxide in dry tetrahydrofuran. Ab initio quantum calculations were also performed to estimate the 13C nuclear

Richard Bos; Neil W. Barnett; Gail A. Dyson; Kieran F. Lim; Richard A. Russell; Simon P. Watson

2004-01-01

136

Flow injection determination of papaverine based on its sensitizing effect on the chemiluminescence reaction of permanganate-sulfite  

Microsoft Academic Search

A novel chemiluminescence (CL) method for the determination of papaverine (PAP) has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite and acidic permanganate. A mechanism for the CL reaction has been proposed on the basis of fluorescent and chemiluminescence spectra. The CL response is proportional to the concentration of

Ya-Feng Zhuang; Si-Chun Zhang; Jun-Sheng Yu; Huang-Xian Ju

2003-01-01

137

Analysis and assessment of the capacity of neutrophils to produce reactive oxygen species in a 96-well microplate format using lucigenin- and luminol-dependent chemiluminescence  

Microsoft Academic Search

The chemiluminescence (CL) assay has been used to measure the reactive oxygen species (ROS)-generating capacity of phagocytes. To achieve more optimal measurement conditions for a multi-channel microplate photon-counting CL analyzer with the cooled charge-coupled device (CCD) camera which offers enhanced sensitivity, we investigated factors affecting the variability in lucigenin-dependent CL (LgCL) measurement of human neutrophils stimulated with either opsonized zymosan

Hiroko Hasegawa; Katsuhiko Suzuki; Shigeyuki Nakaji; Kazuo Sugawara

1997-01-01

138

In vitro generation of reactive oxygen species by free coelomic cells of the annelid Eisenia fetida andrer: An analysis by chemiluminescence and nitro blue tetrazolium reduction  

Microsoft Academic Search

Coelomocytes of the earthworm Eisenia fetida andrei were activated in vitro with various stimulants in order to investigate their capacity to produce reactive oxygen species. Analysis by luminol-enhanced chemiluminescence and nitro blue tetrazolium reduction suggests the production in vitro of reactive oxygen species by both categories of free coelomocytes, leucocytes and chloragocytes, while affecting different modalities: a respiratory burst-like reaction

Pierre Valembois; Maguy Lassègues

1995-01-01

139

The regenerative chemiluminescence of ruthenium complexes in self-oscillating reactions  

Microsoft Academic Search

The usual method for the amplification of chemiluminescence is the introduction of strongly fluorescing activators which accept the energy of the excited species but do not participate in reactions [i]. Another method for the activation of CL involves the use of an activator which itself undergoes cyclic oxidation--reduction transformations resulting in chemical excitation and constant regeneration in the system. In

A. D. Karavaev; V. P. Kazakov; G. A. Tolstikov; V. V. Yakshin; N. L. Khokhlova

1985-01-01

140

A novel chemiluminescence paper microfluidic biosensor based on enzymatic reaction for uric acid determination  

Microsoft Academic Search

In this work, chemiluminescence (CL) method was combined with microfluidic paper-based analytical device (?PAD) to establish a novel CL ?PAD biosensor for the first time. This novel CL ?PAD biosensor was based on enzyme reaction which produced H2O2 while decomposing the substrate and the CL reaction between rhodanine derivative and generated H2O2 in acid medium. Microchannels in ?PAD were fabricated

Jinghua Yu; Shoumei Wang; Lei Ge; Shenguang Ge

2011-01-01

141

Intensity Oscillation of Chemiluminescence in Ferroin-Catalyzed Belousov-Zhabotinsky Reaction  

Microsoft Academic Search

A weak chemiluminescence phenomenon was observed in ferroin-catalyzed Belousov-Zhabotinsky (BZ) reaction. The luminescence intensity oscillated with the self- oscillation of the visible color change and redox potential change in anti phase. A periodic signal of the luminescence was also detected even after the extinction of the self- oscillation. These results showed that the ferroin-catalyzed system has additional oscillatory reaction which

Osamu INOMOTO; Tomoyuki OHYA; Shoichi KAI

2000-01-01

142

Chemiluminescent cholesterol sensor based on peroxidase-like activity of cupric oxide nanoparticles.  

PubMed

A chemiluminescent cholesterol sensor with good selectivity and enhanced sensitivity was constructed based upon the peroxidase-like activity of cupric oxide nanoparticles. Cupric oxide nanoparticles can catalyze the oxidation of luminol by H2O2, which was produced by the reaction of cholesterol and oxygen that was catalyzed by cholesterol oxidase. Therefore, the oxidation of cholesterol could be transduced into the chemiluminescence of luminol by combining these two reactions. Under the optimum conditions, the CL intensity was proportional to the concentration of cholesterol over the range of 0.625-12.5?M and a detection limit was 0.17?M. The applicability of proposed method has been validated by determination of cholesterol in milk powder and human serum samples with satisfactory results. PMID:23274189

Hong, Lei; Liu, Ai-Lin; Li, Guang-Wen; Chen, Wei; Lin, Xin-Hua

2012-12-06

143

Determination of iodide by detection of iodine using gas-diffusion flow injection and chemiluminescence  

Microsoft Academic Search

This work describes development of a flow injection (FI) system for determination of iodide, based on the chemiluminescence (CL) reaction between iodine and luminol. Iodide in the sample zone is oxidized to iodine. Employment of a gas-diffusion (GD) unit allows for selective detection of the generated CL (425nm). Preliminary results showed for concentrations of less than 2mgL?1, that signals were

N. Ratanawimarnwong; N. Amornthammarong; N. Choengchan; P. Chaisuwan; M. Amatatongchai; P. Wilairat; I. D. McKelvie; D. Nacapricha

2005-01-01

144

Investigation of a chemiluminescent system for the determination of ammonia by flow-injection analysis  

SciTech Connect

A novel system for the determination of ammonia based on the chemiluminescent reaction between hypochlorite and luminol is presented. The technique of flow injection analysis was employed to automate the system. Ammonia reacts with hypochlorite to form monochloramine in basic solution which decreases the observed chemiluminescence intensity. Several interferents are identified, and the reasons why they interfere are discussed. The effects of interferents are minimized through the use of a double-tube dialyzer where the ammonia is diffused across the dialyzer membrane into a recipient stream of hydrochloric acid.

Kraus, P.R.; Crouch, S.R.

1987-01-01

145

Selective chlorine dioxide determination using gas-diffusion flow injection analysis with chemiluminescent detection  

SciTech Connect

An automated chemiluminescent technique has been developed utilizing the advantages of gas-diffusion flow injection analysis. A gas-diffusion membrane separates the donor (sampling) stream from the acceptor (detecting) stream and removes ionic interferences. A novel chemiluminescence flow-through detector cell is used to measure the concentration of chlorine dioxide as a function of the intensity of the chemiluminescence produced from its reaction with luminol. The chemiluminescent reagent merges with the analyte directly in front of the photomultiplier tube in order to maximize the sensitivity of the system. The detection limit for chlorine dioxide is approximately 5 ppb. The method is over 1500 times more selective for chlorine dioxide than for chlorine on a mole basis. This method eliminates interference from iron and manganese compounds, as well as other oxychlorinated compounds such as chlorite ion and chlorate ion.

Hollowell, D.A.; Gord, J.R.; Gordon, G.; Pacey, G.E.

1986-06-01

146

Chemiluminescence of Firefly Luciferin without Enzyme  

Microsoft Academic Search

We have been able to produce chemiluminescence in firefly luciferin without utilizing the enzyme luciferase. Following the analogous mechanism of the chemiluminescence of luminol in the organic solvent dimethyl sulfoxide, we have prepared synthetically the methyl ester of luciferin, the phosphate ester of luciferin and luciferyl adenylate by condensation in dimethyl sulfoxide with Khorana's reagent, dicyclohexylcarbodiimide and diazomethane, phosphoric acid,

H. H. Seliger; W. D. McElroy

1962-01-01

147

Fluorimetry studies of oscillating chemilumnescence in the luminol-H2O2-KSCN-CuSO4-TMAOH system.  

PubMed

Oscillating chemical reactions are complex systems, involving a large number of chemical species. In oscillating chemical reaction, some species, usually a reaction intermediate, exhibit fluctuation in its concentration. In this report, visible oscillating chemiluminescence produced by the addition of luminol (3-aminophthalhydrazide) to the oscillating system of H2O2-KSCN-CuSO4-TMAOH was investigated using spectrofluorimetry method. The effects of ingredient concentration of the oscillating system and complexing agents like citric acid and cysteine on the behavior of the oscillating system were investigated. Moreover, the influence of nonaqueous solvents such as ethanol and ethylene glycol has been studied. PMID:15883765

Samadi-Maybodi, Abdolraouf; Naseri, Niloofar; Chaichi, M J

2005-03-01

148

Mechanism of action of 4-dialkylaminopyridines as secondary enhancers in enhanced chemiluminescence reaction.  

PubMed

Kinetic study of peroxidase-catalyzed oxidation of 3-(10'-phenothiazinyl)propane-1-sulfonate (SPTZ) by hydrogen peroxide demonstrated that the addition of 4-dialkylaminopyridines to a substrate solution significantly increased a rate of production of SPTZ cation radical and did not affect the rate of decomposition of this radical. These results explain the mechanism of action of 4-dialkylaminopyridines as secondary enhancers in enhanced chemiluminescence reaction used widely in analytical practice. PMID:23123428

Sakharov, Ivan Yu; Vdovenko, Marina M

2012-10-31

149

Oscillating chemiluminescence in rhodamine B-induced Belousov-Zhabotinsky reaction catalyzed by Ce(IV)  

Microsoft Academic Search

It was found that rhodamine B could induce oscillating chemiluminescence (OCL) from the Ce4+-catalyzed Belousov-Zhabotinsky reaction. This new OCL system, i.e., rhodamine B-malonic acid-bromate-Ce(IV)-sulfuric acid,\\u000a exhibited two clearly distinguished emission peaks in each oscillation period. The initial concentrations of the reactants\\u000a strongly influenced the oscillation pattern. For the study of the CL mechanism, a platform for a versatile and simultaneous

ChunFeng Duan; GuangMing Liu; ZhiFeng Zhang; Hua Cui

2009-01-01

150

Amplified electrochemiluminescence of luminol based on hybridization chain reaction and in situ generate co-reactant for highly sensitive immunoassay.  

PubMed

In this work, we described a simple and highly sensitive electrochemiluminescence (ECL) strategy for IgG detection. Firstly, l-cysteine functionalized reduced graphene oxide composite (l-cys-rGO) was decorated on the glassy carbon electrode (GCE) surface. Then anti-IgG was immobilized on the modified electrode surface through the interaction between the carboxylic groups of the l-cys-rGO and the amine groups in anti-IgG. And then biotinylated anti-IgG (bio-anti-IgG) was assembled onto the electrode surface based on the sandwich-type immunoreactions. By the conjunction of biotin and streptavidin (SA), SA was immobilized, which in turn, combined with the biotin labeled initiator strand (S1). In the presence of two single DNA strands of glucose oxidase labeled S2 (GOD-S2) and complementary strand (S3), S1 could trigger the hybridization chain reaction (HCR) among S1, GOD-S2 and S3. Herein, due to HCR, numerous GOD was efficiently immobilizated on the sensing surface and exhibited excellent catalysis towards glucose to in situ generate amounts of hydrogen peroxide (H2O2), which acted as luminol's co-reactant to significantly enhance the ECL signal. The proposed ECL immunosensor presented predominate stability and high sensibility for determination of IgG in the range from 0.1pgmL(-1) to 100ngmL(-1) with a detection limit of 33fgmL(-1) (S/N=3). Additionally, the designed ECL immunosensor exhibited a promising application for other protein detection. PMID:24054635

Xiao, Lijuan; Chai, Yaqin; Yuan, Ruo; Cao, Yaling; Wang, Haijun; Bai, Lijuan

2013-06-21

151

Atmospheric measurements of nitrogen dioxide with a sensitive luminol instrument  

Microsoft Academic Search

A highly sensitive, lightweight, portable instrument has been developed for continuous monitoring of N02 in air. It operates by detecting the chemiluminescence produced when N02 encounters a surface wetted with a specially formulated solution containing luminol. Unlike other chemiluminescent instruments it measures N02 directly and does not require prior conversion of N02 to NO. It does not respond to H202,

H. I. Schiff; G. I. Mackay; C. Castledine; G. W. Harris; Q. Tran

1986-01-01

152

Sensitized chemiluminescence reaction between hydrogen peroxide and periodate of different types of Mn-doped ZnS quantum dots  

Microsoft Academic Search

In this work, different types of Mn-doped ZnS QDs were prepared and applied to study of the chemiluminescence reaction of\\u000a hydrogen peroxide and periodate. The effects of the size of nanoparticles, shell protection and the stabilizing agents on\\u000a chemiluminescence intensity were studied. The results indicated that the size of quantum dots was not the only factor to enhance\\u000a the intensity

JunXiao Liu; Hui Chen; Ling Lin; Chao Lu; JinMing Lin

2010-01-01

153

Short-Time-Response measurements of nitrogen dioxide and peroxyacetyl nitrate by fast capillary gas chromatography with luminol detection.  

SciTech Connect

The interaction of hydrocarbons and nitrogen oxides in sunlight to produce photochemical smog has been well studied over the years. In the past, the workhorse for the measurement of NO{sub 2}and NO was the chemiluminescent reaction with ozone. This method has detection limits of approximately 0.5 ppb in most commercial instruments, but it cannot detect NO{sub 2} directly; the instrument detects NO and uses hot catalytic surfaces to decompose all other nitrogen oxides (including NO{sub 2}) to NO for detection (l). The main problem with the method is the inherent difficulty in detecting excited NO{sub 2}, which emits over a broad region beginning at approximately 660 nm and has a maximum at 1270 nm, thus requiring a red-shifted photomultiplier for detection. The use of luminol for direct chemiluminescent detection of NO{sub 2} was demonstrated to have greater inherent sensitivity (detection limits of 5 ppt) than the indirect ozone chemiluminescence detection (2). In the luminol system, a gas-liquid reaction leads to light emission with a maximum at approximately 425 nm, at the maximum sensitivity for most photomultiplier tubes. This emission is responsible for the increased detection sensitivities. The biggest problem with this method for direct measurement of NO{sub 2} has been interference due to other soluble oxidants, particularly peroxyacyl nitrates (PANs).

Marley, N. A.; Gaffney, J. S.; Drayton, P. J.

2000-12-07

154

Compact polytetrafluoroethylene assembly-type capillary electrophoresis with chemiluminescence detection.  

PubMed

We have developed a compact polytetrafluoroethylene (PTFE) assembly-type capillary electrophoresis with chemiluminescence (CL) detection system. Luminol-microperoxidase-hydrogen peroxide chemiluminescence reaction was adopted. The device is rectangular in shape (60 mm x 40 mm x 30 mm) and includes three reservoirs (sample, migration buffer, and detection reservoirs) with electrodes. The detection reservoir includes an optical fiber to transport light at the capillary tip to a photomultiplier tube. Isoluminol isothiocyanate (ILITC) was analyzed as a model using this device with fused-silica or polytetrafluoroethylene capillary tubes 10 cm in length. We also used the sample reservoir as a reactor for an immune reaction between anti-human serum albumin immobilized on glass beads and isoluminol isothiocyanate-labeled human serum albumin. The present polytetrafluoroethylene assembly with the capillary tube was useful as a palm-sized analysis device for separation and detection, as well as a reactor. PMID:16870196

Tsukagoshi, Kazuhiko; Ishida, Shingo; Oda, Yuichi; Noda, Keiichi; Nakajima, Riichiro

2006-07-25

155

Recent applications of electrogenerated chemiluminescence in chemical analysis.  

PubMed

Analytical applications of electrogenerated chemiluminescence (ECL) are reviewed with emphasis on the years 1997-2000. Recent developments are described for the ECL of organics, metal complexes and clusters, cathodic ECL on oxide covered electrodes, ECL based immunosensors, DNA-probe assays and enzymatic biosensors. Mechanisms are given for polyaromatic hydrocarbons, luminol/hydrogen peroxide, some cathodic ECL reactions and ruthenium complexes with and without co-reactants. New developments and improvements of techniques and instrumentation and their application to analytes are described. The application of ECL for visualisation of electrochemical processes and imaging of surfaces is mentioned. PMID:18968276

Fähnrich, K A; Pravda, M; Guilbault, G G

2001-05-30

156

Chemiluminescence accompanied by the reaction of gold nanoparticles with potassium permanganate.  

PubMed

It was found that potassium permanganate (KMnO(4)) could react with gold nanoparticles in a strong acid medium to generate particle size-dependent chemiluminescence (CL). For gold nanoparticles with the size of 2.6 or 6.0 nm, the reaction was fast and could produce the excited state Mn(II) with light emission around 640 nm. For gold nanoparticles larger than 6.0 nm, no light emission was observed due to a much slower reaction rate. The CL intensity was found to increase linearly with the concentration of 2.6 nm gold nanoparticles. The effects of the acid medium, concentration of KMnO(4) and presence of N(2) and O(2) were investigated. UV-Vis absorption spectra and X-ray photoelectron spectra (XPS) measured before and after the CL reaction were analyzed. A CL mechanism has been proposed suggesting that the potassium permanganate was reduced by gold nanoparticles in the strong acid medium to the excited state Mn(II), yielding light emission. The results bestow new light on the size-dependent chemical reactivities of the gold nanoparticles and on nanoparticle-induced chemiluminescence. The CL reaction was considered to be of potential use for bioanalysis applications. PMID:16482345

Zhang, Zhi-Feng; Cui, Hua; Shi, Ming-Juan

2005-12-08

157

Chemiluminescent logic gates based on functionalized gold nanoparticles/graphene oxide nanocomposites.  

PubMed

Label-free logic gates (AND, OR, and INHIBIT) based on chemiluminescence (CL) as new optical readout signal have been developed by taking advantage of the unique CL activity of luminol- and lucigenin-functionalized gold nanoparticles/graphene oxide (luminol-lucigenin/AuNPs/GO) nanocomposites. It was found that Fe(2+) ions could induce the CL emission of luminol-lucigenin/AuNPs/GO nanocomposites in alkaline solution. On this basis, by using Fe(2+) ions and NaOH as the inputs and the CL signal as the output, an AND logic gate was fabricated. When the initial reaction system contained luminol-lucigenin/AuNPs/GO nanocomposites and NaOH, either Fe(2+) ions or Ag(+) ions could react with the luminol-lucigenin/AuNPs/GO nanocomposites to produce a strong CL emission. This result was used to design an OR logic gate using Fe(2+) ions and Ag(+) ions as the inputs and CL signal as the output. Moreover, two INHIBIT logic gates for Fe(2+) and Ag(+) were also developed using by NaClO and L-cysteine as their CL inhibitors, respectively. Furthermore, the proposed logic gates were successfully used to detect Fe(2+) , Ag(+) , and L-cysteine, respectively. The developed logic gates may find future applications in sensing, clinical diagnostics, and environmental monitoring. PMID:23963613

He, Yi; Cui, Hua

2013-08-21

158

Chemical reactions of atomic hydrogen at SiC surface and heterogeneous chemiluminescence  

NASA Astrophysics Data System (ADS)

In studies of the surface properties of SiC polytypes and chemical reactions of hydrogen atoms at SiC surfaces the surface (chemi)luminescence of SiC has been applied excited in the reaction of hydrogen atoms due to chemical energy released (heterogeneous chemiluminescence, HCL). The bulk photoluminescence (PL) have also been used for comparison with surface HCL. All the samples showed HCL, but only ?-SiC (6H, 15R), technologically or specially doped (predominantly by N, B, Al), exhibited PL (?ex=365 nm). Cubic polycrystalline ?-SiC (or 3C SiC) did not show PL. The general luminescence band of ?-SiC (6H, 15R) and B and Al doped SiC (6H) was a broad yellow band with ?max ranged from 620 to 650 nm for PL (110 K). Another less intensive luminescence band is a blue one, which has been observed only at low temperatures for ?-SiC (6H,15R) and B and Al doped SiC (6H) in PL spectra and as a shoulder in HCL spectra (?max=477 nm at 110 K for 15R SiC). The green band near 540 nm was also observed sometimes in PL spectra for ?-SiC. The heat of adsorption of hydrogen atoms at polycrystalline ?-SiC estimated from HCL data was found to be in the range from 2 to 3 eV.

Styrov, V. V.; Tyutyunnikov, V. I.; Sergeev, O. T.; Oya, Y.; Okuno, K.

2005-02-01

159

A new chemiluminescence method for the determination of nickel ion  

NASA Astrophysics Data System (ADS)

A new chemiluminescence (CL) phenomenon described as the second-chemiluminescence (SCL) was observed and a strong CL signal was detected, when Ni(II) ion was injected into the mixture after the end of the reaction of potassium permanganate with alkaline luminol. The possible CL mechanism is proposed based on the kinetic curve of the CL reaction, CL spectra, UV-vis spectra and some other experiments. A flow-injection analysis for the determination of nickle(II) ion has been developed, based on the catalysis of nickel(II) ion on the CL reaction between potassium manganate produced on-line and luminol under alkaline condition. Under the optimum conditions, the SCL intensity is linear with the concentration of nickel(II) ion in the range of 8.0-200.0 ?g l -1 and 0.2-2.0 mg l -1. The R.S.D. was 4.5% for 11 determinations of 250 ?g l -1 nickel(II) ion and the detection limit (3 ?) for nickel(II) ion was 0.33 ?g l -1. The method was applied to determine nickel(II) ion in synthetic samples with satisfactory results.

Li, Li Na; Li, Nian Bing; Luo, Hong Qun

2006-05-01

160

Chemiluminescence determination of gemifloxacin based on diperiodatoargentate (III)-sulphuric acid reaction in a micellar medium.  

PubMed

A novel flow-injection chemiluminescence (FI-CL) analysis method for the determination of gemifloxacin in the presence of cetyltrimethylammonium bromide (CTAB) surfactant micelles is described. Strong CL signal was generated during the reaction of gemifloxacin with diperiodatoargentate (III) in a sulfuric acid medium sensitized by CTAB. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of gemifloxacin from 1.0 × 10(-9) to 3.0 × 10(-7) g/mL and the detection limit was 7.3 × 10(-10) g/mL (3?). The relative standard deviation (RSD) was 1.7 % for a 3.0 × 10(-8) g/mL gemifloxacin solution (11 repeated measurements). The proposed method was successfully applied to the determination of gemifloxacin in pharmaceutical preparations and biological fluids. The possible mechanism of the CL reaction is also discussed briefly. PMID:22362634

Zhao, Fang; Zhao, Wen-hui; Xiong, Wei

2012-02-24

161

Chemiluminescence detection of peroxynitrite with flow injection  

NASA Astrophysics Data System (ADS)

Peroxynitrite is an important derivative made by nitric oxide in vivo. It can make damages in many kinds of tissue and cells. Its research value in heart disease and cancer is a very high. A sensitive, specific method for analysis of peroxynitrite is described. In this method, chemiluminescence reaction between perodynitrite and luminol was used to detect with flow injection system. The assay has a detection limit of 2 by 10-8 mol L-1, and linear range of 5 by 10-8 mol L-1 to 5 by 10-5 mol L-1. The application o f flow injection system offers the possibility to establish biosensor for real-time detection of perodynitrite.

Kang, Dai; Evmiridis, Nick P.; Vlessidis, Athanasios; Zhou, Yikai

2001-09-01

162

Determination of trace amounts of iron in water by a chemiluminescence method  

SciTech Connect

The authors used the chemiluminescent oxidation reaction of luminol with hydrogen peroxide to determine iron in waters of various composition (neutral mine, artesian, and deionized waters). The oxidation reaction takes place in a weakly alkaline medium, and the catalyst is iron (III) activated by TETA and DETA. The iron content is evaluated from the intensity of the luminescence. The method is distinguished by its low detection limit (0.04 ng/ml), sufficient selectivity, stability of the luminescence with time, and accessibility of the reagents.

Pilipenko, A.T.; Bogoslovskaya, a T.A.; Terletskaya, A.V.

1986-09-01

163

Chemiluminescence detection with separation techniques for bioanalytical applications  

Microsoft Academic Search

Chemiluminescence detection is known to be a sensitive, selective, and versatile method that can be used in combination with\\u000a separation techniques such as high-performance liquid chromatography, capillary electrophoresis, and chip electrophoresis.\\u000a This article reviews the bioanalytical applications of a combination of chemiluminescence detection and separation techniques\\u000a published in the literature between 1999 and 2008. Luminol chemiluminescene, peroxyoxalate chemiluminescence, and electrochemiluminescence

Makoto Tsunoda

2009-01-01

164

Simultaneous flow-injection determination of iron(II) and total iron by micelle enhanced luminol chemiluminescence 1 Presented at the Flow Analysis VII, 25–28 August 1997, Piracicaba, Brazil. 1  

Microsoft Academic Search

A flow-injection method is proposed for the determination of iron(II) and for the simultaneous determination of iron(II) and total iron based on the catalytic effect of iron(II) on the oxidation of luminol with hydrogen peroxide in an alkaline medium. The activating effect for the catalysis of iron(II) was extremely exhibited in the presence of citric acid, while citric acid acted

Kumiko Saitoh; Takashi Hasebe; Norio Teshima; Makoto Kurihara; Takuji Kawashima

1998-01-01

165

Measurement of NAD(P)H oxidase-derived superoxide with the luminol analogue L-012  

Microsoft Academic Search

In the present study we sought to determine the ability of the chemiluminescence dye 8-amino-5-chloro-7-phenylpyridol[3,4-d]pyridazine-1,4-(2H,3H)dione sodium salt (L-012) to detect superoxide in different biological systems. In human whole blood or isolated leukocytes, the sensitivity of the luminol analogue L-012 to detect superoxide was higher as compared with luminol, lucigenin, coelenterazine, and the fluorescence dye dihydroethidine. In isolated leukocytes as well

Andreas Daiber; Michael August; Stephan Baldus; Maria Wendt; Matthias Oelze; Karsten Sydow; Andrei L Kleschyov; Thomas Munzel

2004-01-01

166

The NO + O and NO + O sub 3 reactions. 2. Analysis of NO sub 2 continuum chemiluminescence  

SciTech Connect

The NO{sub 2}* continuum chemiluminescence from the NO + O and NO + O{sub 3} reactions is simulated by using the model presented in the preceding paper. The model's validity is supported by the good agreement between calculated and experimental NO + O continuum spectra. A calculation of NO + O{sub 3} chemiluminescence assuming reaction on the ground potential energy surface alone is found to give reasonable agreement with experimental data on the continuum shape, absolute intensity, half-quenching pressure, and activation energy. This finding casts doubt on the popular hypothesis that NO{sub 2}* is formed in the NO + O{sub 3} reaction via an excited electronic channel.

Adler-Golden, S.M. (Spectral Sciences, Inc., Burlington, MA (USA))

1989-01-26

167

Determination of copper(II) based on its catalytic effect on thiosemicarbazide-H(2)O(2)-CTMAB chemiluminescence reaction.  

PubMed

A novel chemiluminescence (CL) reaction, thiosemicarbazide (TSC)-H(2)O(2), for the determination of copper at nanogram per milliliter level in batch type is described. The method is based on the catalytic effect of copper(II) on the oxidation of TSC with hydrogen peroxide to produce light emission. The emitted light was observed by using a conventional fluorescence detector. In the optimum conditions, calibration graph was linear in the range of 0.1-1.3ppm. The limit of detection was 10ppb. The relative standard deviation for five determinations of 0.5ppm copper(II) was 1.93%. The proposed method permitted the selective and sensitive determination of Cu(II) in human hair and wheat flour with sufficient precision. The possible mechanism for the new chemiluminescence reaction was also discussed. PMID:18970098

Sorouraddin, M H; Manzoori, J L; Iranifam, M

2005-06-15

168

Flow injection determination of papaverine based on its sensitizing effect on the chemiluminescence reaction of permanganate-sulfite.  

PubMed

A novel chemiluminescence (CL) method for the determination of papaverine (PAP) has been developed by combining the flow injection technique and its sensitizing effect on the weak CL reaction between sulfite and acidic permanganate. A mechanism for the CL reaction has been proposed on the basis of fluorescent and chemiluminescence spectra. The CL response is proportional to the concentration of PAP over the range 0.2-10 micro mol L(-1). The detection limit of PAP is 0.10 micro mol L(-1) (3 s) with a relative standard deviation (RSD) of 2.0% for 10 repetitive determinations of 1.0 micro mol L(-1) PAP. Interferences from other alkaloids in the opium, such as morphine and codeine, are negligible except that of narcotine. The method has been satisfactorily used for the determination of PAP in injections and compound liquorice tablets. PMID:12560974

Zhuang, Ya-Feng; Zhang, Si-Chun; Yu, Jun-Sheng; Ju, Huang-Xian

2002-11-26

169

Determination of copper(II) based on its catalytic effect on thiosemicarbazide–H 2O 2–CTMAB chemiluminescence reaction  

Microsoft Academic Search

A novel chemiluminescence (CL) reaction, thiosemicarbazide (TSC)–H2O2, for the determination of copper at nanogram per milliliter level in batch type is described. The method is based on the catalytic effect of copper(II) on the oxidation of TSC with hydrogen peroxide to produce light emission. The emitted light was observed by using a conventional fluorescence detector. In the optimum conditions, calibration

M. H. Sorouraddin; J. L. Manzoori; M. Iranifam

2005-01-01

170

A Greener Chemiluminescence Demonstration  

ERIC Educational Resources Information Center

|Because they are dramatic and intriguing, chemiluminescence demonstrations have been used for decades to stimulate interest in chemistry. One of the most intense chemiluminescent reactions is the oxidation of diaryl oxalate diesters with hydrogen peroxide in the presence of a fluorescer. In typical lecture demonstrations, the commercially…

Jilani, Osman; Donahue, Trisha M.; Mitchell, Miguel O.

2011-01-01

171

Flow-Injection Chemiluminescence Sensor for the Determination of Free Chlorine in Tap Water  

Microsoft Academic Search

A novel chemiluminescence(CL) sensor combined with flow-injection analysis has been developed for determining free chlorine in tap water. The analytical reagent luminol was immobilized on an anion exchange resin column. While a volumn of sodium hydroxide passed through the column, luminol was eluted from the resin in alkaline aqueous solution and then mixed with a sample stream to react and

Wei Qin; Zhujun Zhang; Shuna Liu

1997-01-01

172

New method for monitoring nitric oxide in vivo using microdialysis sampling and chemiluminescence reaction  

NASA Astrophysics Data System (ADS)

A new method employing a combination of micro dialysis sampling and chemiluminescence reaction was developed to monitor nitric oxide (NO) in vivo. A special probe was designed with an interference-free membrane to achieve a very high selectivity for NO. High sensitivity was achieved by optimizing the working system and improving the NO sampling time. This system was used in vivo to monitor blood and brain tissue in rats and rabbits. We have established that this system is sensitive enough to detect variations in NO production in difference physiological state. The system can detect NO in the linear range of 5nM-1(mu) M, with a detection limit of 1nM, and real NO concentrations in our experimental animals were found to be in the range of 1-5 nM or even less. Finally, the effects of body temperature, NO donors, Viagra, NO activators, NO cofactors, NO interference were investigated carefully in different physiological situations.

Yao, Dachun; Evmiridis, Nick P.; Zhou, Yikai; Xu, Shunqing; Zhou, Huarong

2001-09-01

173

Synergism and chemiluminescence of cerium ions and ruthenium complexes in the belousov-zhabotinskii reaction  

Microsoft Academic Search

This paper studies chemiluminescence (CL) in the system BrO⁻â-CHâ (COOH)â -Ce\\/sup 3 +\\/,4+-RuPbipy)â \\/SUP 2+,\\/ â. The tests were carried out in a CL³ unit that included a light-tight chamber, a photoelectron multiplier (FEU-97), a VS-22 high voltage power pack, and an EPPV-60M recording potentiometer. The synergism in chemiluminescence at low concentrations of ruthenium complex does not appear in the

A. D. Karavaev; V. P. Kazakov; G. A. Tolstikov

1986-01-01

174

Chemiluminescence energy transfer reaction for the on-line preparation of peroxymonocarbonate and Eu(II)-dipicolinate complex.  

PubMed

In this work, an on-line preparation of peroxymonocarbonate was formed innovatively, which offered a reliable intermediate for further investigation. The forming conditions of on-line peroxymonocarbonate ions were investigated in detail. Meanwhile, the energy transfer chemiluminescent reaction of peroxymonocarbonate and the Eu(II)-dipicolinate complex was studied. Through UV-visible absorption spectra, CL method, ESR spin-trapping technique, and mass spectrum experiments, it can be concluded that peroxymonocarbonate oxidizes Eu(II) to Eu(III), and simultaneously creates radicals. The bond rearrangement within radicals formed the singlet molecular oxygen. The energy originating from the singlet oxygen was accepted by the (Eu(III)dipic)- complex. The excited (Eu(III)dipic)- ions underwent radiative deactivation and emitted the chemiluminescence. The peroxymonocarbonate system was a simple, inexpensive, and relatively nontoxic alternative to other oxidants, and it can be used in a mild, neutral-pH environment. PMID:16759143

Liu, Meilin; Zhao, Lixia; Lin, Jin-Ming

2006-06-15

175

Two Techniques for Eliminating Luminol Interference Material and Flow System Configurations for Luminol and Firefly Luciferase Systems.  

National Technical Information Service (NTIS)

Two methods for eliminating luminol interference materials are described. One method eliminates interference from organic material by pre-reacting a sample with dilute hydrogen peroxide. The reaction rate resolution method for eliminating inorganic forms ...

R. R. Thomas

1976-01-01

176

Measurement of salivary cortisol by a chemiluminescent organic-based immunosensor.  

PubMed

A highly sensitive chemiluminescent immunoassay (CLIA) using a sensitive organic photodetector was developed to detect human cortisol, an important biomarker for stress-related diseases. The developed CLIA was performed onto gold-coated glass chips, on which anti-cortisol antibodies were immobilised and chemiluminescent horseradish peroxidase-luminol-peroxide reactions were generated. Using cortisol-spiked artificial saliva samples, the CLIA biosensor showed a linear range of detection between 0.1 ng/mL and 175 ng/mL and a detection limit of 80 pg/mL. The sensor response was highly specific to cortisol and did not vary significantly between assays. The results indicate the potential clinical application of the CLIA sensor. Furthermore, the simple layered structure of the organic photodetector may encourage the realisation of integrated optical biosensors for point-of-use measurement of salivary cortisol levels. PMID:24092121

Pires, N M M; Dong, T

2013-01-01

177

A homogeneous immunoassay of thyroxine based on microchip electrophoresis and chemiluminescence detection.  

PubMed

A homogeneous chemiluminescent immunoassay of thyroxine (T4) present in serum samples is described. The proposed method deployed the competitive immunoreaction of T4 and horseradish peroxidase (HRP)-labeled T4 (HRP-T4) with anti-T4 mouse monoclonal antibody (Ab). HRP-T4 and the HRP-T4-Ab complex were separated and quantified by using microchip electrophoresis (MCE) with chemiluminescence (CL) detection. The MCE separation was accomplished within 60 s. Highly sensitive CL detection was achieved by means of HPR-catalyzed luminol-H(2)O(2) reaction. The linear range for T4 was 5-250 nM with a detection limit of 2.2 nM (S/N = 3). PMID:22976092

Zhao, Shulin; Liu, Yi-Ming

2013-01-01

178

Continuous flow determination of carbon dioxide in water by membrane separation-chemiluminescent detection  

SciTech Connect

Carbon dioxide has been found to enhance the chemiluminescence of a luminol system. A determination method for carbon dioxide in water was developed by applying this reaction to a continuous flow membrane-separation system. Concentrations of carbon dioxide as low as 0.04 ..mu..g C/mL were determined. Membrane-separation effectively eliminated interferences from Co(II), Cr(III), Fe(III), and other ions which also enhance chemiluminescence. The relative standard deviation for this method was 2.8% (n=5) for 4.0 ..mu..g C/mL and the time required for the analysis of one sample was 3.0 min.

Aoki, T.; Ito, K.; Munemori, M.

1988-10-01

179

Detection of mitochondria-derived reactive oxygen species production by the chemilumigenic probes lucigenin and luminol  

Microsoft Academic Search

Both lucigenin and luminol have widely been used as chemilumigenic probes for detecting reactive oxygen species (ROS) production by various cellular systems. Our laboratory has previously demonstrated that lucigenin localizes to the mitochondria of rat alveolar macrophages and that lucigenin-derived chemiluminescence (CL) appears to reflects superoxide (O??2) production by mitochondria in the unstimulated macrophages. In this study, we further examined

Yunbo Li; Hong Zhu; Michael A. Trush

1999-01-01

180

Electrocatalysis of anodic, oxygen-transfer reactions at noble metal electrodes  

SciTech Connect

Voltammetry at rotated disk and rotated ring-disk electrodes was applied to the study of several aspects of anodic, oxygen-transfer reactions at noble electrodes. Anodic reactions which involve the transfer of oxygen from H{sub 2}O to the oxidation products generally exhibit a voltammetric response characterized by severe kinetic limitations. Mechanistic studies were performed at noble electrodes in order to contrive strategies for improving the kinetics of these reactions. Competitive adsorption studies were used to devise an adsorption hierarchy for Au rotated disk electrodes. It was concluded that adsorption was a prerequisite for oxidations involving the transfer of oxygen present on the electrodes surface as adsorbed hydroxyl radicals. The electrogenerated chemiluminescence (ECL) of luminol was studied at Au, Pt, Pd, glassy carbon, PbO{sub 2}, and Bi-doped PbO{sub 2} electrodes. The ECL intensity was determined to be inversely related to electrochemical activity for the oxidation of luminol. It was concluded that the oxygen-transfer oxidation of luminol to 3-aminophthalate ( n = 4 eq mol{sup {minus}1}) corresponded to the dark reaction, whereas the electron-transfer oxidation of luminol with n = 1 eq mol{sup {minus}1} initiated the chemiluminescent reaction in solution.

Vitt, J.E.

1992-06-09

181

Flow-injection analysis chemiluminescence detection combined with microdialysis sampling for studying protein binding of drug  

Microsoft Academic Search

The bovine serum album binding of streptomycin sulfate was studied in vitro using the technique of microdialysis combined with flow-injection analysis-chemiluminescence detection. The principle of the determination of streptomycin sulfate is that it increases the radiation emitted during the chemiluminescence oxidation of luminol by potassium hexacyanoferrate (III) in sodium hydroxide medium. The drug and protein were mixed in different molar

Yuming Huang; Zhujun Zhang; Daojian Zhang; Jiagen Lv

2001-01-01

182

Chemiluminescence and free-radical stages of the Belousov-Zhabotinskii reaction  

Microsoft Academic Search

The profound effect of stirring on chemiluminescence of the system: bromate-malonic acid-ruthenium complexes with low (?10?5 M) catalyst concentrations indicates the significant contribution of organic radicals R. to the oscillation mechanism. Critical radical concentration for this oscillation is [R.]C?10 M.

A. D. Karavaev; V. P. Kazakov

1987-01-01

183

Highly sensitive chemiluminescent point mutation detection by circular strand-displacement amplification reaction  

Microsoft Academic Search

Single nucleotide polymorphism (SNP) genotyping is attracting extensive attentions owing to its direct connections with human diseases including cancers. Here, we have developed a highly sensitive chemiluminescence biosensor based on circular strand-displacement amplification and the separation by magnetic beads reducing the background signal for point mutation detection at room temperature. This method took advantage of both the T4 DNA ligase

Chao Shi; Yujie Ge; Hongxi Gu; Cuiping Ma

2011-01-01

184

Application of optimized chemiluminescence assay for determination of the antioxidant capacity of herbal extracts.  

PubMed

A chemiluminescence (CL) assay for the determination of antioxidant capacity (AOC) has been optimized and applied to analyses of herbal extracts in the present study. The optimal concentrations of reagents (luminol, H2O2, horseradish peroxidase) have been determined, as well as the optimal reaction conditions (wavelength, pH, temperature, sample volume). All of the measurements were performed at the emission maximum of the oxidized form of luminol (425?nm). The optimal concentrations of the reagents were determined as follows: 1.6?mmol/L luminol, 7.5?mmol/L H2O2 and 0.14 U/mL horseradish peroxidase activity in the reaction mixture. Analyses were carried out in phosphate buffer, pH 7.4, at room temperature. With the optimized CL assay, the AOCs of various water and methanol herbal extracts were determined (dog rose hips, plantain leaves and coltsfoot and thyme flowers) and the results were compared to those obtained by other classical methods for the evaluation of antioxidants. Strong correlations (r?>?0.9) with the Folin-Ciocalteau assay and the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH)(?) assay are confirmed, although there is no correlation between AOC and the concentration of ascorbic acid in the samples analysed. This optimized CL assay is simple, rapid and reliable, and it represents a good alternative to classical methods (Folin-Ciocalteau, DPPH(?)) for the determination of AOC of herbal extracts and other food samples. PMID:22290787

Poga?nik, Lea; Ulrih, Nataša Poklar

2012-01-30

185

Chemiluminescence flow sensor for folic acid with immobilized reagents  

NASA Astrophysics Data System (ADS)

A novel chemiluminescence (CL) sensor for folic acid combined flow-injection (FI) technology was presented in this paper. The analytical reagents involved in the CL reaction, including luminol and hexacyanoferrate(III), were both immobilized on an anion-exchange column in FI system. The CL signal produced by the reaction between luminol and hexacyanoferrate(III), which were eluted from the column through sodium phosphate injection, was decreased in the presence of folic acid. The CL emission was correlated with the folic acid concentration in the range from 0.01 to 15 ?g ml -1, and the detection limit was 3.5 ng ml -1 folic acid (3 ?). At a flow rate of 2.0 ml min -1, including sampling and washing, could be performed in 2 min with a relative standard deviation of <2.5%. The flow sensor could be reused more than 300 times and has been applied to the analysis of folic acid in pharmaceutical preparations, and the recovery was from 97.4% to 100.4%.

Song, Zhenghua; Zhou, Xin

2001-11-01

186

Postmortem interval of skeletal remains through the detection of intraosseal hemin traces. A comparison of UV-fluorescence, luminol, Hexagon-OBTI®, and Combur® tests.  

PubMed

With the goal of obtaining additional practically applicable methods for estimating the PMI of skeletal remains, 39 samples of human and 5 samples of domestic animal long bones with known PMI (PMI=1 to approximately 2000 years) were tested with two established methods (UV-fluorescence of a freshly sawn cross-section and the luminol test) and two screening tests (Hexagon-OBTI® test and Combur® test) that were being tried out in this context for the first time. The hypothesis underlying this experiment was the supposition that the PMI-related chemiluminescence of the luminol reaction for bone is based on the presence of persisting hemin from hemoglobin molecules in bone. Our results showed that lack of luminescence and reduced UV-fluorescence were more meaningful results for estimating PMI and excluding forensic relevance than a positive luminol reaction or strong UV-fluorescence, as both of the latter findings revealed the limitations of these methods in this particular context. Particularly for cases showing a positive luminol reaction, the use of additional absolute dating methods may be indicated. Against our expectations, both the Combur® test strips and the Hexagon-OBTI® test, which were both devised to demonstrate blood, delivered negative results for all samples. They are thus not suitable for estimating the PMI of skeletal remains. Future research will be necessary to elucidate whether the negative results obtained for these tests may be due to the poor solubility of potentially present hemoglobin or hemoglobin breakdown products in the Tris buffer used in this experiment. PMID:21237592

Ramsthaler, Frank; Ebach, Sarah C; Birngruber, Christoph G; Verhoff, Marcel A

2011-01-15

187

Development and application of a luminol-based nitrogen dioxide detector  

SciTech Connect

An instrument for the continuous measurement of nitrogen dioxide (NO/sub 2/) at all atmospheric concentration ranges and conditions was developed. The detector is based on the chemiluminescent reaction between 5-amino-2,3-dihydro-1,4-phthalazinedione (luminol) and NO/sub 2/ in alkaline aqueous solution. Development included the optimization of the cell design and the solution composition. Sodium sulfite (Na/sub 2/SO/sub 3/) and methanol (CH/sub 3/OH) were added to the solution to improve sensitivity and specificity. The detector was favorably compared to two different instruments measuring NO/sub 2/ by NO + O/sub 3/ chemiluminescent and by a tunable diode laser absorption spectrometry system. The detector has demonstrated a detection limit of 30 parts-per-trillion by volume (ppt) and a frequency response of 0.3 Hz. The instrument was operated for two one-month periods on Bermuda. The purpose was to study air masses from the East Coast of the United States after transport over the ocean. Average daily values were 400 ppt with values as low as 100 ppt measured. Other field experiments involved monitoring of NO/sub 2/ in ambient air in the range of 1 to 60 parts-per-billion by volume.

Wendel, G.J.

1985-01-01

188

Electrogenerated Chemiluminescence  

NASA Astrophysics Data System (ADS)

In electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), electrochemically generated intermediates undergo a highly exergonic reaction to produce an electronically excited state that then emits light. These electron-transfer reactions are sufficiently exergonic to allow the excited states of luminophores, including polycyclic aromatic hydrocarbons and metal complexes, to be created without photoexcitation. For example, oxidation of [Ru(bpy)3]2+ in the presence of tripropylamine results in light emission that is analogous to the emission produced by photoexcitation. This review highlights some of the most exciting recent developments in this field, including novel ECL-generating transition metal complexes, especially ruthenium and osmium polypyridine systems; ECL-generating monolayers and thin films; the use of nanomaterials; and analytical, especially clinical, applications.

Forster, Robert J.; Bertoncello, Paolo; Keyes, Tia E.

2009-07-01

189

Studies on the mechanism of the peroxyoxalate chemiluminescence reaction: part 2. Further identification of intermediates using 2D EXSY 13C nuclear magnetic resonance spectroscopy.  

PubMed

Further consideration has been given to the reaction pathway of a model peroxyoxalate chemiluminescence system. Again utilising doubly labelled oxalyl chloride and anhydrous hydrogen peroxide, 2D EXSY (13)C nuclear magnetic resonance (NMR) spectroscopy experiments allowed for the characterisation of unknown products and key intermediate species on the dark side of the peroxyoxalate chemiluminescence reaction. Exchange spectroscopy afforded elucidation of a scheme comprised of two distinct mechanistic pathways, one of which contributes to chemiluminescence. (13)C NMR experiments carried out at varied reagent molar ratios demonstrated that excess amounts of hydrogen peroxide favoured formation of 1,2-dioxetanedione: the intermediate that, upon thermolysis, has been long thought to interact with a fluorophore to produce light. PMID:18420048

Tonkin, Sarah A; Bos, Richard; Dyson, Gail A; Lim, Kieran F; Russell, Richard A; Watson, Simon P; Hindson, Christopher M; Barnett, Neil W

2008-03-13

190

Chemiluminescence in neutrophils and Lettr? cells induced by myxoviruses.  

PubMed Central

Luminol-mediated chemiluminescence in neutrophils is stimulated by Sendai virus and by influenza virus; Lettré cells also exhibit chemiluminescence (less than 10% of that of neutrophils), which is stimulated by Sendai virus and by influenza virus. Virally induced permeability changes are not responsible for chemiluminescence, since (i) extracellular Ca2+ inhibits permeability changes but stimulates chemiluminescence, and (ii) influenza virus, which induces permeability changes at pH 5.3 but not at pH 7.4, induces chemiluminescence at either pH. Other agents [zymosan, N-formyl-L-methionyl-L-leucyl-L-phenylalanine, 4-phorbol 12-myristate 13-acetate (phorbol ester), A23187] likewise induce chemiluminescence in the absence of permeability changes.

Mehta, S; Bashford, C L; Knox, P; Pasternak, C A

1985-01-01

191

Continuous measurement of nitrous acid (HONO) in indoor environment using a diffusion scrubber and chemiluminescence method  

NASA Astrophysics Data System (ADS)

Recent study has demonstrated that the use of combustion appliances in indoor environments, e.g., gas stoves and heaters, results in significant concentrations of NO2 and nitrous acid (HONO). Indoor HONO is formed by both direct emissions from combustion processes and the heterogeneous reaction of NO2 with water vapor on surfaces present indoors. In this study in-situ instrument was constructed for measuring HONO concentration in both indoor and outdoor environments, utilizing diffusion scrubber and peroxynitrite-induced luminol chemiluminescent methods. We measured the HONO concentration under the conditions existing in living room of an apartment, along with NO, NO2, temperature, and relative humidity, to investigate the sources, chemical transformation, and lifetimes of nitrogen oxides and HONO. Some experiments investigated the emissions and transformations of nitrogen species from operation of unvented or vented gas appliance. Measurement data of NO, NO2, and HONO will be reported, and formation pathway of the HONO under the experimental conditions will also be discussed. In addition to measurement of indoor HONO, comparison of HONO measurements by luminol chemiluminescence and annular denuder integrated samples was made in outdoor environment. HONO in ambient air was sampled with annular denuders (Teflon-coated PM2.5 cyclone inlet followed by two Na2CO3-coated denuders coupled in series) operated at 16.7 L/min. Acknowledgement This study was supported by grant No. (# R01-2005-000-10775-0) from the Basic Research Program of the Korea Science and Engineering Foundation (KOSEF).

Park, S.; Hong, J.; Lee, J.; Cho, S.

2006-12-01

192

[The chemiluminescent detection of activity in peripheral blood neutrophils under allergic diseases of lungs in children with fungous sensibilization].  

PubMed

The high mobilization activity of neutrophils as a capacity of momentary feed-back reaction to factually any external stimuli, make it possible to use them in various diagnostic systems in vitro. Among them is the automated test system of chemiluminescent detection of functional activity of peripheral blood neutrophils in parallel response to luminol, opsonizing zymosan and different antigens. This approach makes it possible to evaluate the initial and induced activity of phagocytes and its dynamics in case of adding in vitro to test system of one or another allergen. The application of micro technique of chemiluminescent detection of functional activity of peripheral blood neutrophils revealed the stimulating effect of allergens Aaspergillus fumigatus, Alternaria alternata, Cladosporum herbatum, Candida albicans and Penicillinum notatum. This is an additional evidence of high sensitivity of children with allergic diseases of lungs to fungi and hence one more factor of diagnostics and treatment. PMID:23265057

Semenova, A V; Minenkova, T A; Mizernitski?, Iu L

2012-10-01

193

Chemiluminescence detection of a phase response to an oxygen perturbation in the Ru(bpy) 32+ catalyzed Belouzov-Zhabotinskii reaction  

NASA Astrophysics Data System (ADS)

The external oxygen perturbation that imposed on the Belouzov-Zhabotinskii (BZ) reaction has been investigated in a closed system by the detection of the chemiluminescence of Ru(bpy) 32+ used as a catalyst. When the perturbation is applied and then removed, the system exhibits phase transitions between perturbed and unperturbed oscillatory states which can be observed directly by detecting the chemiluminescence of the catalyst. Phase shifts caused by the phase transition into the unperturbed oscillations (recovery) have been analyzed in terms of phase response curves.

Saigusa, Hiroyuki

1989-05-01

194

FLOW INJECTION CHEMILUMINESCENCE-BASED CHEMICAL SENSORS  

Microsoft Academic Search

In recent years, the design of chemiluminescence flow injection sensors with immobilized and solid-state reagents has been one of the most active areas in chemiluminescence analysis. In these systems, analytes are detected by the chemiluminescence reactions either with the immobilized reagents directly or with the dissolved reagents which are released from the immobilized substrates or the solid-state forms by some

Wei Qin

2002-01-01

195

Chemiluminescent determination of 1,3-dibromo-5,5-dimethylhydantoin and 1,3-dichloro-5,5-dimethylhydantoin in water and in air  

SciTech Connect

It was found that 1,3-dichloro-5,5-dimethylhydantoin (DCDH) and 1,3-dibromo-5,5-dimethyl-hydantoin (DBDH) react with luminol in aqueous solutions and in organic solvents giving luminous radiation. The optimal conditions for the reaction have been found. A chemiluminescent method was developed for the determination of micro-quantities of DCDH and DBDH in aqueous solutions with detection limits of 0.2 and 4 ng/ml, respectively. The method was used for the analysis of the DBDH content in water and in air in production premises.

Pilipenko, A.T.; Zui, O.V.; Terletskaya, A.V.

1986-10-10

196

A rapid test for heroin (3,6-diacetylmorphine) based on two chemiluminescence reactions.  

PubMed

A rapid method for screening drug seizure samples for 3,6-diacetylmorphine (heroin), which consists of a simple hydrolysis procedure and flow-injection analysis with two chemiluminescence reagents, is described. Before hydrolysis, 3,6-diacetylmorphine evokes an intense response with a tris(2,2'-bipyridyl)ruthenium(III) reagent (prepared by dissolving the perchlorate salt in acetonitrile), and a relatively weak chemiluminescence response with a second reagent: potassium permanganate in an aqueous acidic polyphosphate solution. However, the permanganate reagent is extremely sensitive toward the hydrolysis products of 3,6-diacetylmorphine (i.e., 6-monoacetylmorphine and morphine). Some compounds commonly found in drug laboratories may cause false positives with tris(2,2'-bipyridyl)ruthenium(III), but do not produce the markedly increased response with the permanganate reagent after the hydrolysis procedure. The combination of these two tests therefore provides an effective presumptive test for the presence of 3,6-diacetylmorphine, which we have verified with 14 samples obtained from a forensic science laboratory. PMID:17018085

Agg, Kent M; Craddock, Adrian F; Bos, Richard; Francis, Paul S; Lewis, Simon W; Barnett, Neil W

2006-09-01

197

The forensic luminol test for blood: unwanted interference and the effect on subsequent analysis  

Microsoft Academic Search

When no bloodstains are found by visual inspection in crime scene investigations the chemilumine- scence (CL) produced in the luminol test for blood is a useful aid. The complex reactions that give rice to the blue- green CL are facilitated by the catalytical activity of the hemoglobin in blood. The luminol test has a high sensitivity compared to other forensic

Anders Nilsson

198

Fast GC-Luminol Instrument for PAN and Nitrogen Dioxide Measurements  

NASA Astrophysics Data System (ADS)

Fast gas chromatography (GC) with luminol detection was used to determine nitrogen dioxide and peroxyacetyl nitrate (PAN) levels onboard the National Oceanic and Atmospheric Administration Twin Otter during the Bay Region Atmospheric Chemistry Experiment (BRACE) in Tampa, Florida, in May 2002. Nitrogen dioxide concentrations measured by the fast GC-luminol technique were compared with simultaneous onboard NO2 determinations made by photolytic conversion to NO with subsequent ozone chemiluminescence detection. Use of photon counting (Hamamatsu HC-135) and an automated gas sampling valve enabled PAN measurement at 30-sec intervals. The fast GC-luminol system with photon counting will be described briefly. We recently improved the instrument and modified the outflow of the luminol carrier gas to minimize luminol spillage. Results will be presented for PAN during BRACE, and calibration data will be shown for the latest version of the instrument. The potential for luminol modification to improve sensitivity to PAN will also be discussed. This work was supported by the U.S. Department of Energy's Atmospheric Science Program.

Marley, N. A.; Gaffney, J. S.

2003-12-01

199

CMOS arrays as chemiluminescence detectors on microfluidic devices.  

PubMed

A simple, low-cost process to integrate complementary metal oxide semiconductor array detectors (CMOSAD) for chemiluminescence is presented, evaluated, and applied to the determination of nitrite in ground water samples. CMOS arrays of different brands (obtained from commercial image sensors) were adapted as chemiluminescence detectors on microfluidic devices. The performance of the CMOSADs was evaluated in the visible zone of the spectrum using a tungsten halogen lamp as light source. Intrinsic parameters assessed included signal stability, spectral response, dark current, and signal-to-noise ratio. Thereafter, the CMOSADs were integrated on microfluidic devices and their performances in quantitative analysis were assessed with the chemiluminometric reaction of hydrogen peroxide with luminol, catalyzed with hexacyanoferrate (III). The parameters assessed were sensitivity, linear range, detection limit, reproducibility, correlation coefficient of the calibration curves, and baseline drift during measurements. The CMOSAD with the best performance was selected to assess the applicability of the developed microfluidic devices with the integrated detector. The microfluidic system permitted the determination of nitrite with both good precision and good recovery values in the analysis of ground water samples. Integration was easily achieved and enabled the development of a simple, low-cost, and feasible alternative to conventional detectors. PMID:20177663

Rodrigues, Eunice R G O; Lapa, Rui A S

2010-02-23

200

Sequential injection chemiluminescence immunoassay for anionic surfactants using magnetic microbeads immobilized with an antibody.  

PubMed

A rapid and sensitive immunoassay for the determination of linear alkylbenzene sulfonates (LAS) is described. The method involves a sequential injection analysis (SIA) system equipped with a chemiluminescence detector and a neodymium magnet. Magnetic beads, to which an anti-LAS monoclonal antibody was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of the magnetic beads in the flow cell were controlled by means of a neodymium magnet and adjusting the flow of the carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an anti-LAS monoclonal antibody on the magnetic beads and the LAS sample and horseradish peroxidase (HRP)-labeled LAS, and was based on the subsequent chemiluminscence reaction of HRP with hydrogen peroxide and p-iodophenol, in a luminol solution. The anti-LAS antibody was immobilized on the beads by coupling the antibody with the magnetic beads after activation of a carboxylate moiety on the surface of magnetic beads that had been coated with a polylactic acid film. The antibody immobilized magnetic beads were introduced, and trapped in the flow cell equipped with the neodymium magnet, an LAS solution containing HRP-labeled LAS at constant concentration and the luminol solution were sequentially introduced into the flow cell based on an SIA programmed sequence. Chemiluminescence emission was monitored by means of a photon counting unit located at the upper side of the flow cell by collecting the emitted light with a lens. A typical sigmoid calibration curve was obtained, when the logarithm of the concentration of LAS was plotted against the chemiluminescence intensity using various concentrations of standard LAS samples (0-500ppb) under optimum conditions. The time required for analysis is less than 15min. PMID:18970310

Zhang, Ruiqi; Hirakawa, Koji; Seto, Daisuke; Soh, Nobuaki; Nakano, Koji; Masadome, Takashi; Nagata, Kazumi; Sakamoto, Kazuhira; Imato, Toshihiko

2005-12-15

201

Bovine neutrophils recruited by endotoxin to a teat cistern continuously produce oxygen radicals and show increased phagocytosis and extracellular chemiluminescence  

Microsoft Academic Search

Bovine neutrophils were harvested from a teat cistern following endotoxin infusion and were compared with blood neutrophils by measurements of chemiluminescent and phagocytic activity towards C3-and IgG-opsonized and unopsonized yeast particles. Both phagocytosis and luminol-dependent chemiluminescence elicited by all three particles were enhanced in the teat cells. The increase in the luminoldependent chemiluminescence towards C3-and IgG-opsonized particles was due to

Charlotte Hallén Sandgren; Ingrid Larsson; Karin Persson

1992-01-01

202

Chemiluminescence determination of trimetazidine via inducing the aggregation of gold nanoparticles  

NASA Astrophysics Data System (ADS)

A simple, rapid and sensitive chemiluminescence (CL) method combined with flow injection analysis was developed for the determination of trimetazidine. Trimetazidine was found to significantly increase the CL signal arising from N-bromosuccinimide-luminol reaction in the presence of gold nanoparticles. The enhanced CL intensity was proportional to trimetazidine concentration in the range of 0.01-5.0 ?g/mL, with a limit of detection (3sb) of 6.7 ng/mL. The relative standard deviation was 2.8% for 11 repetitive measurements of 0.1 ?g/mL trimetazidine solution. The practicality of the method was evaluated by determining trimetazidine in pharmaceutical formulations and in spiked human serum samples. Moreover, the possible CL reaction mechanism was also discussed.

Li, Jiao; Quan, Jie; Du, Jianxiu; Liu, Mei

2013-10-01

203

Molecular beam chemiluminescence studies of the NO + O/sub 3/ reaction and modeling of global NO/sub 2/ distribution  

SciTech Connect

The results of a crossed molecular beam study of the chemiluminescent reaction NO + 0/sub 3/ ..-->.. NO/sub 2/ + 0/sub 2/ are discussed. The chemiluminescence as a function of collision energy and an excitation function were obtained using a translationally cooled supersonic NO beam. An investigation into the role of the internal energy states using an effusive NO beam and a supersonic O/sub 3/ beam has been presented. The results show that chemiluminescence enhancement occurs when high and low temperature NO experiments are compared. The role that other energy modes may have is discussed. The observed enhancement is consistent with the concept that the chemiluminescence cross section increases with NO molecular rotation for low J states. The second part discusses the role of NO/sub 2/ in preserving a global ozone balance. NO/sub 2/ vertical profiles based on Noxon's (1979) column measurements were derived. The method of instantaneous rates was used to calculate the rate of ozone production and destruction by O/sub x/ and NO/sub x/ on a grid that covered the entire globe. The results were presented as a function of altitude and latitude in contour plots.

Kowalczyk, M.

1980-11-01

204

1-Ethyl-3-methylimidazolium ethylsulfate/copper catalyst for the enhancement of glucose chemiluminescent detection: effects on light emission and enzyme activity.  

PubMed

The effect of the ionic liquid 1-ethyl-3-methylimidazolium ethylsulfate ([Emim][EtSO(4)]) on the copper-catalyzed luminol chemiluminescence (CL) is reported. A drastic light emission enhancement is observed, related to a strong interaction between Cu(2+) and the imidazolium ring. In these conditions, the CL reaction was able to produce light efficiently at pH as low as 6.5 (amplification factor: Intensity(+IL)/Intensity(-IL) = 2900). Interesting effects of [Emim][EtSO(4)] on the enzyme glucose oxidase activity were also evidenced, and advantages were taken from this enhancement to perform sensitive chemiluminescent glucose detection (LOD = 4 microM) at pH 8.0. PMID:20163148

Santafé, Aurélie A-M; Doumèche, Bastien; Blum, Loïc J; Girard-Egrot, Agnès P; Marquette, Christophe A

2010-03-15

205

Fast gas chromotography with luminol detection for measurement of nitrogen dioxide and PANs  

Microsoft Academic Search

Fast capillary gas chromatography has been coupled to a luminol-based chemiluminescence detection system for the rapid monitoring of nitrogen dioxide and peroxyacyl nitrates. A first-generation instrument was described recently (Gaffney et al., 1998). This system is capable of monitoring nitrogen dioxide and peroxyacyl nitrates (PANs; to and including the C4 species) with 1-min time resolution. This is an improvement by

Jeffrey S. Gaffney; Nancy A. Marley; Paul J. Drayton

1999-01-01

206

Dynamic component chemiluminescent sensor for assessing circulating polymorphonuclear leukocyte activity of peritoneal dialysis patients.  

PubMed

Recurrent bacterial peritonitis is a major complication in peritoneal dialysis (PD) patients, which is associated with polymorphonuclear leukocyte (PMN) functional changes and can be assessed by a chemiluminescent (CL) reaction. We applied a new approach of a dynamic component chemiluminescence sensor for the assessment of functional states of PMNs in a luminol-amplified whole-blood system. This method is based on the evaluation of CL kinetic patterns of stimulated PMNs, while the parallel measurements of intracellular and extracellular production of reactive oxygen species (ROS) from the same sample can be conducted. Blood was drawn from diabetic and nondiabetic patients during follow-up, and during peritonitis. Healthy medical personnel served as the control group. Chemiluminescence curves were recorded and presented as a sum of three biological components. CL kinetic parameters were calculated, and functional states of PMNs were assessed. Data mining algorithms were used to build decision tree models that can distinguish between different clinical groups. The induced classification models were used afterward for differentiating and classifying new blind cases and demonstrated good correlation with medical diagnosis (84.6% predictive accuracy). In conclusion, this novel method shows a high predictive diagnostic value and may assist in detection of PD-associated clinical states. PMID:18510343

Prilutsky, Daria; Rogachev, Boris; Vorobiov, Marina; Zlotnik, Moshe; Last, Mark; Lobel, Leslie; Marks, Robert S

2008-05-30

207

Phenylboronic acid immunoaffinity reactor coupled with flow injection chemiluminescence for determination of alpha-fetoprotein.  

PubMed

A reusable and sensitive immunoassay based on phenylboronic acid immunoaffinity reactor in combination with flow injection chemiluminescence (CL) for determination of glycoprotein was described. The reactor was fabricated by immobilizing 3-aminophenylboronic acid (APBA) on glass microbeads with gamma-glycidoxypropyltrimethoxysilane (GPMS) as linkage. The alpha-fetoprotein (AFP) could be easily immobilized on the APBA coated beads through sugar-boronic interaction. After an off-line incubation, the mixture of the analyte AFP with horseradish peroxidase-labeled AFP antibody (HRP-anti-AFP) was injected into the reactor. This led the trapping of free HRP-anti-AFP by the surface coated AFP on glass beads. The trapped HRP-anti-AFP was detected by chemiluminescence due to its sensitizing effect on the reaction of luminol and hydrogen peroxide. Under optimal conditions, the chemiluminescent signal was proportional to AFP concentration in the range of 10-10 0 ng m L(-1). The whole assay process including regeneration of the reactor could be completed within 31 min. The proposed system showed acceptable detection and fabrication reproducibility, and the results obtained with the present method were in acceptable agreement with those from parallel single-analyte test of practical clinical sera. The described method enabled a low-cost, time saving and was potential to detect the serum AFP level in clinical diagnosis. PMID:19012831

Wu, Yafeng; Zhuang, Yafeng; Liu, Songqin; He, Lin

2008-10-14

208

Chemiluminescence of benzoic and cinnamic acids, and flavonoids in the presence of aldehyde and hydrogen peroxide or hydroxyl radical by fenton reaction  

Microsoft Academic Search

Light emission (chemiluminescence; CL) was observed by exciting phenolic compounds with hydrogen peroxide (H2O2) or hydroxyl radical (HO·), formed from H2 and FeCl2 by the Fenton reaction. The intensity of the CL of benzoic acid derivatives in the presence of (H2 was in the order of gallic acid > caffeic acid > 3,5-dimethoxy-4-hydroxycinnamic acid > isovanillic acid = syringic acid.

Yumiko Yoshiki; Kazuyoshi Okubo; Masamichi Onuma; Kiharu Igarashi

1995-01-01

209

New Chemical Analysis Methods Based on Redox Reactions Coupled with Chemiluminescence Measurements,  

National Technical Information Service (NTIS)

The fundamental chemistry of gold-catalyzed oxidation/reduction reactions of organic and inorganic compounds with nitrogen dioxide and nitric acid has been studied to better understand the surface chemistry, effects of reaction conditions, catalyst config...

R. S. Hutte

1985-01-01

210

Electrochemiluminescence of luminol at the titanate nanotubes modified glassy carbon electrode.  

PubMed

A new strategy for the construction of a sensitive and stable electrochemiluminescent platform based on titanate nanotubes (TNTs) and Nafion composite modified electrode for luminol is described, TNTs contained composite modified electrodes that showed some photocatalytic activity toward luminol electrochemiluminescence emission, and thus could dramatically enhance luminol light emission. This extremely sensitive and stable platform allowed a decrease of the experiment electrochemiluminescence luminol reagent. In addition, in luminol solution at low concentrations, we compared the capabilities of a bare glassy carbon electrode with the TNT composite modified electrode for hydrogen peroxide detection. The results indicated that compared with glassy carbon electrode this platform was extraordinarily sensitive to hydrogen peroxide. Therefore, by combining with an appropriate enzymatic reaction, this platform would be a sensitive matrix for many biomolecules. PMID:24073426

Xu, Guifang; Zeng, Xiaoxue; Lu, Shuangyan; Dai, Hong; Gong, Lingshan; Lin, Yanyu; Wang, Qingping; Tong, Yuejin; Chen, Guonan

211

Development of a chemiluminescence detector for analysis of nitrite in biological samples  

NASA Astrophysics Data System (ADS)

A new instrument for the determination of low concentrations of nitrite is presented. Nitrite reacts with I- in acidic medium to form nitric oxide that is subsequently removed from reaction solution by scrubbing with argon gas, then nitric oxide is detected by Luminol/H2O2 Chemiluminescence detection. The detection limit of nitrite is 10pmol for S/N equals 3. The calibration graph is linear up to 1000 pmol. The coefficient of variation for 500pmol nitrite in samples is 4.87 percent. Analysis time for single test is 250 S. The results are in good agreement with a spectrophotometric interfering ions and has been applied to the analysis of a variety of biological samples.

Liu, Zhiwei; Zhou, Yikai; Xu, Shunqing; Ren, Shu; Zhang, Zhiren

2001-09-01

212

Determination of Cr(III) in urine, blood serum and hair using flow injection chemiluminescence analysis  

Microsoft Academic Search

A flow injection (FI) chemiluminescence method for the determination of Cr(III) in blood serum, urine and hair samples is\\u000a reported. It is based on the chromium-catalyzed light emission from the luminol oxidation by hydrogen peroxide. The apparatus\\u000a consists of an FI system with a flow cell formed by a coiled transparent tube suitable for chemiluminescence detection. The\\u000a specificity of the

R. Escobar; M. S. García-Domínguez; A. Guiraúm; F. F. de la Rosa

1998-01-01

213

How the morphology of biochips roughness increases surface-enhanced chemiluminescence  

NASA Astrophysics Data System (ADS)

Biochips of Au particle arrays were fabricated using nanosphere lithography to investigate the influence of gold roughness upon chemiluminescence induced by peroxidase adsorbed at surface. The corrugation of gold induces a strong enhancement of the chemiluminescence of luminol brought at its vicinity. This letter shows that this enhancement is increased by an order of magnitude when the corrugation is regular and possesses an average curvature radius of around 15 nm (for ordered particles arrays compared to films with random roughness).

Shen, Hong; Lu, Guowei; Ou, Meigui; Marquette, Christophe A.; Ledoux, Gilles; Roux, Stéphane; Tillement, Olivier; Perriat, Pascal; Cheng, Bolin; Chen, Zhenghao

2007-05-01

214

[Chemiluminescence decrease of singlet oxygen in NaClO+H2O2 reaction in the presence of different anti-cataract drugs].  

PubMed

Singlet oxygen was produced in chemical reaction NaClO+ H2O2. Action of different well-known anti-cataract drugs on this reaction was studied. There is no doubt that the singlet oxygen chemiluminescence decreases in the presence of Catalin and Baineiting. Finnish Catachrom Ophthan, Vita iodurol (France) and Quinax (USA) have no such effect at all which may be a result of the interaction of these remedies with H2O2 and/or with NaClO. PMID:1793850

Shvachko, A G; Formaziuk, V E; Sergienko, V I

1991-07-01

215

Investigation on enhanced chemiluminescence reaction systems with bis(hydrogenperiodato) argentate(III) complex anion for fluoroquinolones synthetic antibiotics.  

PubMed

A novel chemiluminescence (CL) reaction system with bis(hydrogenperiodato) argentate(III) complex anion (Ag(III) complex, [Ag(HIO(6))(2)](5-)), for the first time, is developed for the determination of lomefloxacin (LMFX), enrofloxacin (ENLX) and pefloxacin (PFLX). The possible CL emission mechanism was discussed by comparing the fluorescence emission with CL spectra. The CL conditions of [Ag(HIO(6))(2)](5-)-H(2)SO(4)-LMFX/ENLX/PFLX systems were investigated and optimized. Under the optimized experimental conditions, the CL intensity is proportional to the concentration of the drugs in the range 0.2994-36.80x10(-7)g mL(-1) for LMFX, 4.00-30.0x10(-7)g mL(-1) for ENLX and 1.54-27.64x10(-7)g mL(-1) for PFLX. The limit of detection (s/n=3) was 9.1x10(-9)g mL(-1) for LMFX, 3.1x10(-9)g mL(-1) for ENLX and 4.4x10(-9)g mL(-1) for PFLX. The recovery of LMFX, ENLX and PELX from the spiked pharmaceutical preparations was in the range of 92.3-105% with the RSDs of 0.5-2.7%. For urine, serum and milk samples the recoveries of the three drugs were in the range of 85.1-107% for LMFX with the RSDs of 2.3-3.4%. 80.2-112% for ENLX with the RSDs of 1.4-2.8%, and 87.8-114% for PFLX with the RSDs of 1.6-2.7%. The proposed method was applied successfully to the determination of these compounds in real samples. PMID:19559854

Sun, Hanwen; Chen, Peiyun; Wang, Fei; Wen, Haifang

2009-03-14

216

3-(10'-Phenothiazinyl)propionic acid is a potent primary enhancer of peroxidase-induced chemiluminescence and its application in sensitive ELISA of methylglyoxal-modified low density lipoprotein.  

PubMed

Using a full factorial design the optimization of experimental conditions of enhanced chemiluminescence reaction (ECR) catalyzed by horseradish peroxidase (HRP) in the presence of 3-(10'-phenothiazinyl)propionic acid (PPA) as a primary enhancer was performed. The effect of concentrations of PPA, hydrogen peroxide, MORPH, luminol, and Tris on a ratio of peroxidase-catalyzed CL to background was studied. The detection limit value of HRP in ECR with PPA was 0.09pM. Using PPA the ultra-sensitive chemiluminescent ELISA for determination of methylglyoxal-modified low density lipoprotein was developed. The detection limit value for the developed method was 0.5ngmL(-1). The obtained results open up very promising perspectives for using PPA to improve the sensitivity of enzyme immunoassay kits. PMID:24054611

Sakharov, Ivan Yu; Demiyanova, Alexandra S; Gribas, Anastasia V; Uskova, Natalia A; Efremov, Evgeny E; Vdovenko, Marina M

2013-06-07

217

An Integrated Microfluidic System with Chemiluminescence Detection for Single Cell Analysis after Intracellular Labeling  

PubMed Central

This work describes the first application of microchip electrophoresis with chemiluminescence detection (MCE-CL) in single cell analysis. Human red blood cells were assayed to determine intracellular content of glutathione (GSH). Intracellular GSH was first labeled by incubating cells with diazo-luminol, and then individual cells were injected, in-line lysed, and MCE separated. CL detection was based on the oxidation reaction of luminol - labeled GSH with NaBrO. The MCE-CL assay had a linear calibration curve over a range from 0.2~ 90 amol GSH injected with a correlation coefficient of 0.9991 and a detection limit of 50 zmol or 3.6× 10?9 M (S/N = 3). The average content of GSH in individual human red blood cells was found 64.9 amol (n= 17). Compared with the MCE methods with laser induced fluorescence detection (LIF) reported so far for single cell analysis, the present MCE-CL assay of GSH is simple and about 100 times more sensitive.

Zhao, Shulin; Li, Xiangtang

2009-01-01

218

HTFFR kinetics studies of Sn\\/N2O, a highly efficient chemiluminescent reaction  

Microsoft Academic Search

High temperature fast-flow reactors (HTFFR) were used to study the Sn\\/N2O reaction from 300–950 K at pressures from 4 to 110 Torr. The observed emissions are SnO[a 3?+(1) –X 1?+] and (b 3&Pgr;–X 1?+). The photon yield of the former system is 0.53±0.26 independent of T, that of the latter (5.9±2.9) ×10?1 exp[?(1200±200)\\/T]. Comparison of the photon yields of N2O-

William Felder; Arthur Fontijn

1978-01-01

219

Homogeneous gas phase chemiluminescence measurement of reactive hydrocarbon air pollutants by reaction with oxygen atoms  

Microsoft Academic Search

A continuous flow method for measuring total (photochemical smog) reactivity of dilute hydrocarbon mixtures is described. The differences between the OH emission intensities at 308.9 and 312.2 nm from O-atom\\/hydrocarbon reactions near 1 torr is measured. For CâHâ, I\\/sub 308.9\\/ much greater than I\\/sub 312.2\\/ while for CâHâ, Iâââ.â approximately I\\/sub 312.2\\/. All other hydrocarbons, HC (olefinic, dienic, paraffinic, and

A. Fontihn; Roy Ellison

1975-01-01

220

Determination of polyamines by flow injection analysis with a chemiluminescence detector based on their complexation with copper(II).  

PubMed

Through the flow injection analysis experiments, we discovered that an unsaturated complex of Cu(II) and polyamines (spermine, spermidine, putrescine) had a strongly catalytic effect on luminol-H(2)O(2) chemiluminescence (CL) reaction, and that the CL intensity is proportional to the concentrations of polyamines. Based on the automatic formation of an unsaturated complex of polyamines and Cu(II) when the solution containing polyamines passed through a column packed with solid Cu(OH)(2), a new flow injection chemiluminescence analysis method was proposed for the determination of polyamines. The effects of pH, buffer concentration, the concentration of chemiluminescence reagent, and the influence of mixing coil length were examined. Under optimal conditions, the linear range was from 1.0 x 10(-7) mol L(-1) to 1.0 x 10(-5) mol L(-1), and the detection limits were 0.17, 0.38, 0.44 pmol for spermine, spermidine, and putrescine, respectively. Compared with other methods, the advantages of this method include convenience, time-saving and low cost. PMID:16770059

Li, Zheng-Ping; Wu, Qiu-Hua; Wang, Chun; Su, Yu-Qin

2006-05-01

221

Novel FIA chemiluminescence fiber optic biosensor for urinary and blood glucose  

Microsoft Academic Search

A chemiluminescence fiber optic biosensor system coupled to FIA was developed to measure glucose in bodily fluids. Glucose oxidase was immobilized on a preactivated nylon membrane and attached to the tip of a fiber optic bundle. This enzyme acts on (beta) -D-glucose to produce hydrogen peroxide which was then reacted with luminol in the presence of ferricyanide to produce a

Maurice V. Cattaneo; J. H. Luong

1993-01-01

222

Reusable light-emitting-diode induced chemiluminescence aptasensor for highly sensitive and selective detection of riboflavin.  

PubMed

A novel reusable chemiluminescence aptasensor was developed based on aptamer recognition coupled with light-emitting-diode induced chemiluminescence (LED-CL) detection. The sensing approach was based on the design that the model analyte riboflavin (Rf) in sample solutions was captured by the immobilized aptamers and then eluted simply with alkaline luminol solution to catalyze the CL reaction between luminol and dissolved oxygen under high power LED irradiation. This design allowed a very simple (branch-free) flow way for the CL sensing system. The CL intensity versus the Rf concentration was linear in the range from 0.03 to 5ngmL(-1) with a limit of detection (LOD) down low to 8pgmL(-1). Without renewing the aptamer, the relative standard deviation (RSD) for seven consecutive detections is 2.33%; the sensor also showed good stability without performance deterioration after >100 times use. Up to 10000-fold of K(+), Na(+), Ca(2+), Mg(2+), and Fe(3+), 5000-fold for glucose and bovine serum albumin, 1000-fold of uric acid, 1500-fold of ascorbic acid (added with Fe(3+)), 100-fold of flavin mononucleotide and 200-fold of flavin adenine dinucleotide caused no significant interference with the determination of 0.5ngmL(-1) Rf. The sensor was applied for analysis of Rf in urine and food samples with the recovery of 94-103%. The advantages of reusability, simplicity, high sensitivity and selectivity provided by the LED-CL aptasensor will make it a good alternative tool for biological and food analysis. PMID:23298628

Xu, Shuxia; Zhang, Xinfeng; Liu, Weiwei; Sun, Yonghua; Zhang, Heliang

2012-12-13

223

How surface-enhanced chemiluminescence depends on the distance from a corrugated metal film  

NASA Astrophysics Data System (ADS)

Peroxidase labeled streptavidin was immobilized onto the surface of bulk and clusterlike metal films at a distance controlled by a peptide chain with a length between 1.3 and 7.8 nm. Luminol chemiluminescence which occurred at peroxidase vicinity depends on the metal nanostructure. When peroxidase is attached on a bulklike film, chemiluminescence increases monotonously with the distance because of a decrease of the light emission quenching by metal. When peroxidase is attached on a clusterlike film, chemiluminescence undergoes a complex variation with the metal/catalyst distance evidencing a competition between the already mentioned quenching process and a nanostructure-induced catalysis enhancement.

Lu, Guowei; Shen, Hong; Cheng, Bolin; Chen, Zhenghao; Marquette, Christophe A.; Blum, Loic J.; Tillement, Olivier; Roux, Stéphane; Ledoux, Gilles; Ou, Meigui; Perriat, Pascal

2006-11-01

224

Evaluation of the probes 2?,7?-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation  

Microsoft Academic Search

This study attempts to provide a critical assessment of three different common approaches to identifying reactive species formed in biological systems: the 2?,7?-dichlorofluorescin diacetate (DCFH-DA) assay, and the luminol- and lucigenin-amplified chemiluminescence assays. There have been several contradictory reports about the specificity of these methods. Our results show that DCFH is oxidized to the fluorescent compound 2?,7?-dichlorofluorescin (DCF) in human

Oddvar Myhre; Jannike M. Andersen; Halvor Aarnes; Frode Fonnum

2003-01-01

225

Perturbation of the tris(2,2'-bipyridine) ruthenium(II)-catalyzed Belousov-Zhabotinsky oscillating chemiluminescence reaction by L-cysteine and its application.  

PubMed

Perturbation of the tris(2,2'-bipyridine)ruthenium(II) [Ru(bpy)(3)2+]-catalyzed Belousov-Zhabotinsky (BZ) oscillating chemiluminescence (CL) reaction induced by L-cysteine was observed in the closed system. It was found that the CL intensity was decreased in the presence of L-cysteine. Meanwhile, oscillation period and oscillating induction period were prolonged. The sufficient reproducible induction period was used as parameter for the analytical application of oscillating CL reaction. Under the optimum conditions, the changes in the oscillating CL induction period were linearly proportional to the concentration of L-cysteine in the range from 8.0 x 10(-7) to 5.0 x 10(-5 )mol L(-1) (r = 0.997) with a detection limit of 4.3 x 10(-7) mol L(-1). The possible mechanism of L-cysteine perturbation on the oscillating CL reaction was also discussed. PMID:19424964

Liu, Huimin; Han, Heyou

226

Large enhancement of oscillating chemiluminescence with [Ru(bpy)3 ](2+) -catalyzed Belousov-Zhabotinsky reaction in the presence of tri-n-propylamine.  

PubMed

Oscillating chemiluminescence enhanced by the addition of tri-n-propylamine (TPrA) to the typical Belousov-Zhabotinsky (BZ) reaction system catalyzed by ruthenium(II)tris(2.2'-bipyridine)(Ru(bpy)3 (2+) ) was investigated using a luminometry method. The [Ru(bpy)3 ](2+) /TPrA system was first used as the catalyst for a BZ oscillator in a closed system, which exhibited a shorter induction period, higher amplitude and much more stable chemiluminescence (CL) oscillation. The effects of various concentrations of TPrA, oxygen and nitrogen flow rate on the oscillating behavior of this system were examined. In addition, the CL intensity of the [Ru(bpy)3 ](2+) /TPrA-BZ system was found to be inhibited by phenol, thus providing a way for use of the BZ system in the determination of phenolic compounds. Moreover, the possible mechanism of the oscillating CL reaction catalyzed by [Ru(bpy)3 ](2+) /TPrA and the inhibition effects of oxygen and phenol on this oscillating CL system were considered. Copyright © 2012 John Wiley & Sons, Ltd. PMID:23055390

Lan, Xiaolan; Zheng, Baozhan; Zhao, Yan; Yuan, Hongyan; Du, Juan; Xiao, Dan

2012-10-10

227

A nonradioisotope chemiluminescent assay for evaluation of 2-deoxyglucose uptake in 3T3-L1 adipocytes. Effect of various carbonyls species on insulin action.  

PubMed

We have developed a rapid nonradioisotope chemiluminescent assay adapted to high-throughput screening experiments, to evaluate glucose uptake activity in cultured cells. For chemiluminescence quantification of 2-deoxyglucose, we used a luminol oxidation reaction after an enzymatic dephosphorylation of 2-deoxyglucose-6-phosphate. All reactions were performed at 37 °C by consecutive addition of reagents, and the assay is able to quantify 2DG in picomole per well. To confirm the reliability of this method, we have evaluated the dose-effect of insulin, GLUT4 inhibitors and insulin-sensitizing agent on 2DG uptake into 3T3-L1 cells. The results obtained with the assay for 2DG uptake in vitro in the absence or presence of insulin stimulation, were similar to those obtained by the previous radioisotopic and enzymatic methods. We have also used this assay to evaluate the effect of various reactive carbonyl and oxygen species on insulin-stimulated 2DG-uptake into adipocytes. All reactive carbonyl species tested decreased insulin-stimulated glucose uptake in a time- and dose-dependent manner without affecting basal glucose uptake in 3T3-L1 cells. 4-hydroxynonenal was found to be the most potent in the impairment of glucose uptake. This new enzymatic chemiluminescent assay is rapid and useful for measurement of 2DG uptake in insulin-responsive in cultured cells. PMID:22835478

Vidal, Nicolas; Cavaillé, Jean Pierre; Poggi, Marjorie; Peiretti, Franck; Stocker, Pierre

2012-07-23

228

Pervaporation-flow injection with chemiluminescence detection for determination of iodide in multivitamin tablets.  

PubMed

This paper describes the use of a pervaporation (PV) technique in a flow injection (FI) system for selective improvement in iodide analysis. Iodide in the sample zone is oxidized to iodine, which permeates through a hydrophobic membrane. Detection of the diffused iodine is achieved using the chemiluminescent (CL) emission at 425nm that results from the reaction between iodine and luminol. The method was applied for the analysis of some pharmaceutical products, such as nuclear emergency tablets and multivitamin tablets. Ascorbic acid present in multivitamin samples interfered seriously with the analysis, and off-line sample treatment using anion exchange resin was employed to successfully remove ascorbic acid before the analysis. Ascorbic acid was flushed from the column using 0.4M sodium nitrate followed by elution of iodide with 2M sodium nitrate. The detection limit (3S.D.) of the system was 0.5mgl(-1), with reproducibility of 5.2% R.S.D. at 5mgl(-1). Sample throughput was determined as 30injectionsh(-1). There was good agreement between iodide concentrations from extracted samples determined using four different methods, i.e., PV-FI, gas diffusion-flow injection, potentiometry and ICP-MS. A comparison of the analytical features of the developed pervaporation system with these of the previously reported chemiluminescence gas diffusion-flow injection previously reported is also described. PMID:19071665

Nacapricha, D; Sangkarn, P; Karuwan, C; Mantim, T; Waiyawat, W; Wilairat, P; Cardwell, T; McKelvie, I D; Ratanawimarnwong, N

2007-01-16

229

Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates  

PubMed Central

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.

Niazov, Angelica; Freeman, Ronit; Girsh, Julia; Willner, Itamar

2011-01-01

230

Following glucose oxidase activity by chemiluminescence and chemiluminescence resonance energy transfer (CRET) processes involving enzyme-DNAzyme conjugates.  

PubMed

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H(2)O(2). The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose. PMID:22346648

Niazov, Angelica; Freeman, Ronit; Girsh, Julia; Willner, Itamar

2011-10-31

231

Cu2+-imprinted cross-linked chitosan resin as micro-column packing materials for online chemiluminescence determination of trace copper.  

PubMed

The Cu(2+)-imprinted cross-linked chitosan resin was synthesized and the binding characteristic of the resin to Cu(2+) was evaluated. The prepared resin was packed into a micro-glass column and used as micro-separating column. The micro-separating column was connected into the chemiluminescence flow system and placed in front of the window of the photomultiplier tube. Based on the luminol-hydrogen peroxide chemiluminescence system, a flow injection online chemiluminescence method for determination of trace copper was developed and trace Cu(2+) in complex samples was successfully determined. The proposed method improved the shortcomings of chemiluminescence method's poor selectivity. PMID:20629043

Nie, Feng; Hao, Liang; Gao, Mei; Wu, Yingchun; Li, Xinsheng; Yu, Sha

2010-07-13

232

A highly sensitive chemiluminescent metalloimmunoassay for H1N1 influenza virus detection based on a silver nanoparticle label.  

PubMed

A versatile, ultrasensitive chemiluminescent metalloimmunoassay method for detection of H1N1 influenza virus was designed by using silver nanoparticles as an anti-H1N1 labeling tag to strongly amplify the CL signal of luminol. PMID:23999899

Li, Yanxia; Hong, Mei; Qiu, Bin; Lin, Zhenyu; Cai, Zongwei; Chen, Yiting; Chen, Guonan

2013-10-17

233

Some Characteristics of Riboflavin Chemiluminescence.  

National Technical Information Service (NTIS)

Data are presented that describe some characteristics of riboflavin chemiluminescence in the dark in the presence of hydrogen peroxide and osmium trichloride. The reaction, in terms of the light intensity produced, is affected by type of buffer, pH, and c...

R. D. Towner H. A. Neufeld P. B. Shevlin

1969-01-01

234

The Chemiluminescence of Organometallic Compounds  

NASA Astrophysics Data System (ADS)

Studies on the liquid-phase and gas-phase reactions of organometallic compounds accompanied by the emission of light are described systematically and discussed. The influence of the magnetic field on the chemiluminescence of Grignard reagents and the study of the electrochemiluminescence of solutions of organometallic compounds are examined. The ways leading to further development of the field of the chemiluminescence of organometallic compounds and certain possible applications of the phenomenon in the monitoring of industrial processes are discussed. The bibliography includes 80 references.

Tolstikov, Genrikh A.; Bulgakov, Ramil G.; Kazakov, Valeri P.

1985-11-01

235

Chemiluminescent detection of nonisotopic probes.  

PubMed

With recent advances in techniques for detecting chemiluminescent substrates, hybridization with nonisotopic rather than radiolabeled probes is becoming more common. In the Basic Protocol, nylon membranes carrying transferred nucleic acids are prepared for hybridization with biotinylated probes by UV cross-linking. This is a critical step in the procedure and the Support Protocol provides a detailed description of light-source calibration. After hybridization, the target nucleic acid is detected through a series of steps that lead to an enzyme-catalyzed light reaction. The Alternate Protocol describes chemiluminescent detection based upon antibody recognition of digoxigenin-labeled probes. For both biotinylated and digoxigenin-labeled probes, chemiluminescent detection is more sensitive than colorimetric detection and has the added advantage that the membrane can be used for multiple film exposures, then stripped and redetected with different probes. PMID:18265227

Perry-O'Keefe, H; Kissinger, C M

2001-05-01

236

Experimental studies on the chemiluminescence reaction mechanism of carbonate/bicarbonate and hydrogen peroxide in the presence of cobalt(II).  

PubMed

Chemiluminescence (CL) phenomena of carbonates or bicarbonates of potassium, sodium, or ammonium with hydrogen peroxide in the presence of cobalt sulfate were reported. After cobalt(II) solution was injected into the mixture of carbonate/bicarbonate and hydrogen peroxide, a CL signal was given out briefly. The CL conditions of these systems were optimized. The CL reaction mechanisms were studied experimentally by examining the spectrum emitted by the CL system and the effect of various free radical scavengers on CL emission intensity. The results showed that the maximal emission wavelengths of the CO32--H2O2-Co2+ and HCO3--H2O2-Co2+ systems were 440 and 490 nm, respectively. As a result, a radical scavenger of ascorbic acid, thiourea, and superoxide dismutase exhibited different effects on these CL systems. The different CL mechanisms involving the carbon dioxide dimer and the oxygen dimer were revealed, respectively. PMID:18173250

Liang, Shu-Xuan; Zhao, Li-Xia; Zhang, Bo-Tao; Lin, Jin-Ming

2008-01-04

237

Automated chemiluminescence immunoassay measurements  

NASA Astrophysics Data System (ADS)

Chemiluminescence (CL) detection offers potential for high sensitivity immunoassays (CLIAs). Several approaches were attempted to automate CL measurements. Those include the use of photographic film, clear microtitration plates, and magnetic separation. We describe a photon counting detection apparatus that performs (CLIA) measurements. The CL detector moves toward a disposable reaction vessel to create a light-tight seal and then triggers and integrates a CL signal. The capture uses antibody coated polystyrene microparticles. A porous matrix, which is a part of a disposable reaction tray, entraps the microparticle-captured reaction product. The CL signal emanated off the immune complex immobilized by the porous matrix is detected. The detection system is a part of a fully automated immunoassay analyzer. Methods of achieving high sensitivities are discussed.

Khalil, Omar S.; Mattingly, G. P.; Genger, K.; Mackowiak, J.; Butler, J.; Pepe, C.; Zurek, T. F.; Abunimeh, N.

1993-06-01

238

Rapid detection of Escherichia coli O157:H7 inoculated in ground beef, chicken carcass, and lettuce samples with an immunomagnetic chemiluminescence fiber-optic biosensor.  

PubMed

A biosensor was evaluated with regard to its usefulness in the rapid detection of Escherichia coli O157:H7 inoculated in ground beef, chicken carcass, and romaine lettuce samples. The biosensor consisted of a chemiluminescence reaction cell, a fiber-optic light guide, and a luminometer linked to a personal computer in conjunction with immunomagnetic separation. The samples inoculated with E. coli O157:H7 were first centrifuged and suspended in buffered peptone water and then incubated with anti-E. coli O157 antibody-coated magnetic beads and horseradish peroxidase (HRP)-labeled anti-E. coli O157 antibodies to form antibody-coated bead-bacterium-HRP-labeled antibody sandwich complexes. Finally, the sandwich complexes were separated from the samples in a magnetic field and reacted with luminol in the reaction cell. The number of E. coli O157:H7 cells was determined by collecting the HRP-catalyzed chemiluminescence signal from the bead surface through a fiber-optic light guide and measuring the signal with a luminometer. The chemiluminescence biosensor was specific for E. coli O157:H7 in samples containing other bacteria, including Salmonella Typhimurium, Campylobacter jejuni, and Listeria monocytogenes. The chemiluminescence signal was linear on a log scale from 10(2) to 10(5) CFU of E. coli O157:H7 per ml in samples. Detection could be completed within 1.5 h without any enrichment. The detection limits for ground beef, chicken carcass, and lettuce samples were 3.2 x 10(2), 4.4 x 10(2), and 5.5 x 10(2) CFU of E. coli O157:H7 per ml, respectively. PMID:12636312

Liu, Yongcheng; Ye, Jianming; Li, Yanbin

2003-03-01

239

Luminol electrochemiluminescence-based fibre optic biosensors for flow injection analysis of glucose and lactate in natural samples  

Microsoft Academic Search

A fibre optic biosensor based on the electrochemiluminescence of luminol has been developed for glucose and lactate flow injection analysis. The electrochemiluminescence of luminol was generated using a glassy carbon electrode polarised at +425mV vs. a platinum pseudo-reference electrode. After optimisation of the reaction conditions and physicochemical parameters influencing the sensor response, the measurement of hydrogen peroxide could be performed

Christophe A Marquette

1999-01-01

240

An ultrasensitive chemiluminescent immunosensor for the detection of human leptin using hemin/G-quadruplex DNAzymes-assembled signal amplifier.  

PubMed

In this work, we reported a sensitive chemiluminescent immunosensor for the detection of human leptin by using hemin/G-quadruplex DNAzymes to amplify detection signal. In this sensing system, the primary antibody (anti-human leptin) was firstly bound to the 96-well plates, and human leptin and biotinylated secondary antibody were successively combined to form sandwich-type immune complex through specific interactions. Then streptavidin labeled with hemin/G-quadruplex DNAzymes was assembled to the sandwich-type immunocomplex by streptavidin-biotin interaction. The DNAzymes exhibited an excellent catalytic activity to the chemiluminescent reaction of luminol with hydrogen peroxide in strong alkaline solution, leading to significant enhancement in response signal. Under the optimum conditions, the proposed immunosensor showed high sensitivity and selectivity with a low detection limit of 1.9pgmL(-1) and a wide linear response range of human leptin from 10 to 1000pgmL(-1). The immunosensor was used to detect human leptin in serum, and the results were in good agreement with the data obtained by conventional ELISA method. PMID:24148479

He, Yuezhen; Wang, Xiaoxun; Zhang, Yuzhong; Gao, Feng; Li, Yongxin; Chen, Hongqi; Wang, Lun

2013-08-07

241

Application of chemiluminescence to monitoring of trace atmospheric species  

SciTech Connect

This dissertation concerns the development of analytical instrumentation based on gas phase chemiluminescence for the monitoring of nitric acid, methyl nitrate, peroxyacetyl nitrate, and total acidity. Nitric acid was converted to NO and NO{sub 2} by a 400 C glass beads converter and the resulting NO{sub 2} was monitored by a luminol-based detector. A CrO{sub 3} converter was used to convert the NO generated in the system to NO{sub 2} to lower the detection limit of the instrument. The detection limit of the configuration was 0.30 ppb of nitric acid. Peroxyacetyl nitrate (PAN) and NO{sub 2} were separated and detected with a novel gas chromatographic system which did not require compressed gas cylinder. Air that has been scrubbed by passing it over FeSO{sub 4} was used as the carrier gas which eliminates the need for any compressed gas cylinders. The detection limits for the instrument (PAN-GC) were 0.12 ppb for PAN and 0.20 ppb for NO{sub 2}. Methyl nitrate was separated from PAN and NO{sub 2} using a modified version of the PAN-GC. A 200 C quartz converter inserted between the end of the column and the detector in the PAN-GC, converts methyl nitrate and PAN into NO{sub 2} for detection by the luminol-based detector. The detection limits are 0.30 ppb for PAN, 0.30 ppb for methyl nitrate, and 0.20 ppb for NO{sub 2} The development of a total acidity detector based on the reaction of O and F atoms with hydrazoic acid (HN{sub 3}) was also carried out. Several methods for converting atmospheric acidity to HN{sub 3} were tested. These included packed bed, coated filters, and denuder methods. The system was calibrated with nitric acid and hydrochloric acid and the characterization of the response to various organic acids was investigated. The detection limits for nitric acid and for hydrochloric acid were 0.51 ppb and 0.63 ppb, respectively.

Burkhardt, M.R.

1989-01-01

242

Portable centrifugal analyzer for the determination of rapid reaction kinetics  

SciTech Connect

A portable centrifugal analyzer prototype is capable of rapidly initiating reactions and monitoring 17 optical channels as they rotate past a stationary photodetector. An advanced rotor drive permits transfer of discretely loaded sample and reagent into a cuvette within 60 ms. Various rotor designs have been employed to ensure effieicnt mixing concurrent with solution transfer, thus permitting absorbance or luminescence measurements to be made almost immediately after solution contract. Dye-dillution studies have been used to investigate transfer and mixing efficiencies. Rotor designs with parallel access for sample and reagent into the cuvette were found to promote efficient mixing during liquid transfer. The hypochlorite-luminol chemiluminescent reaction served to demonstrate the utility of the system for performing rapid kinetic analyses. Appropriate adjustment of reaction conditions allows first-order reaction half-lives as short as 0.04 s to be measured. 13 figures, 3 tables.

Bostick, W.D.; Bauer, M.L.; McCracken, R.; Mrochek, J.E.

1980-02-01

243

Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence.  

PubMed

A novel method for the detection of trace estradiol valerate (EV) in pharmaceutical preparations and human serum was developed by inhibition of luminol chemiluminescence (CL) by estradiol valerate on the zinc deuteroporphyrin (ZnDP)-enhanced luminol-K3 Fe(CN)6 chemiluminescence system. Under optimized experimental conditions, CL intensity and concentration of estradiol valerate had a good linear relationship in the ranges of 8.0 × 10(-8) to 1.0 × 10(-5) g/mL. Detection limit (3?) was estimated to be 3.5 × 10(-8) g/mL. The proposed method was applied successfully for the determination of estradiol valerate in pharmaceutical preparations and human serum and recoveries were 97.0-105.0% and 95.5-106.0%, respectively. The possible mechanism of the CL system is discussed. PMID:22777916

Liu, Wenwen; Xie, Liangxiao; Liu, Hongshuang; Xu, Shichao; Hu, Bingcheng; Cao, Wei

2012-07-09

244

Microfluidic enzyme immunosensors with immobilised protein A and G using chemiluminescence detection  

Microsoft Academic Search

Affinity proteins were covalently immobilised on silicon microchips with overall dimensions of 13.1×3.2 mm, comprising 42 porous flow channels of 235 ?m depth and 25 ?m width, and used to develop microfluidic immunosensors based on horseradish peroxidase (HRP), catalysing the chemiluminescent oxidation of luminol\\/p-iodophenol (PIP). Different hydrophilic polymers with long flexible chains (polyethylenimine (PEI), dextran (DEX), polyvinyl alcohol, aminodextran) and

Julia Yakovleva; Richard Davidsson; Martin Bengtsson; Thomas Laurell; Jenny Emnéus

2003-01-01

245

Molecular imprinting method for on-line enrichment and chemiluminescent detection of the organophosphate pesticide triazophos  

Microsoft Academic Search

A molecularly imprinted polymer (MIP) was synthesized by the precipitation polymerization method using triazophos (TAP) as\\u000a the template. The MIP can selectively absorb TAP from sample solutions. The absorbed TAP strongly enhances the chemiluminescence\\u000a (CL) of luminol-H2O2. This was applied to design a method for the on-line enrichment and detection of TAP. The change in CL intensity is linearly\\u000a proportional

Chenggen Xie; Hankun Zhou; Shan Gao; Huaifen Li

2010-01-01

246

Evidence of impaired polymorphonuclear leucocyte phagocytic functions and chemiluminescence response in rheumatoid arthritis  

Microsoft Academic Search

In this study, the phagocytic uptake of 3H-thymidine-labelled Staphylococcus aureus and bacterial killing (Bk) by polymorphonuclear leucocytes (PMN) from patients with rheumatoids arthritis (RA) were investigated and compared to the luminol-enhanced chemiluminescence response (LCL) to phorbolmyristic acetate (PMA), a receptor and second message-independent activator of respiratory burst activity in definite or classical rheumatoid arthritis (RA), with regard to the inflammatory

W. Marhoffer; M. Stein; E. Keck; K. Federlin

1994-01-01

247

Bio-bar-code dendrimer-like DNA as signal amplifier for cancerous cells assay using ruthenium nanoparticle-based ultrasensitive chemiluminescence detection.  

PubMed

Bio-bar-code dendrimer-like DNA (bbc-DL-DNA) is employed as a label for the amplification assay of cancer cells in combination with the newly explored chemiluminescence (CL) system of luminol-H(2)O(2)-Ru(3+) and specificity of structure-switching aptamers selected by cell-based SELEX. PMID:20652188

Bi, Sai; Hao, Shuangyuan; Li, Li; Zhang, Shusheng

2010-07-23

248

Blood and Milk Neutrophil Chemiluminescence and Viability in Primiparous and Pluriparous Dairy Cows During Late Pregnancy, Around Parturition and Early Lactation  

Microsoft Academic Search

Extensive studies have shown the polymorphonu- clear leukocytes (PMN) dysfunction inextricably links to parturition. To investigate the effect of parity on PMN function, phorbol 12-myristate 13-acetate (PMA) stimulated luminol-amplified chemiluminescence (CL) and viability of blood and milk PMN were investigated in primiparous and pluriparous dairy cows during peri- parturient period. The CL kinetics of blood and milk PMN and hematological

Jalil Mehrzad; Luc Duchateau; Satu Pyörälä; Christian Burvenich

2002-01-01

249

Chemiluminescence detection from sonodynamic action in vitro and in vivo  

NASA Astrophysics Data System (ADS)

In this work, chemiluminescence method was engaged for the first time to detect the active oxygen spices during sonocynamic action both in vitro and in vivo. We used CLA derivatives, which can efficiently react with singlet oxygen (1O2) or superoxide anion (O2-) to emit light, and luminol, which can be oxidized by a variety of free radicals to emit photons, to real-timely detect oxygen free radical formation in the sonosensitization of two sonosensitizer ATX-70 and HpD. The results show that 1O2 is involved in the sonosensitization. The mechanism of sonosensitizing was discussed. In vivo experiments, tumor imaging by sonodynamic chemiluminescence detection methods was established. This method could have potential applications in clinics for early-stage tumor diagnosis.

He, Yonghong; Xing, Da; Yan, Guihong; Ueda, Kenichi

2001-10-01

250

Introducing novel amorphous carbon nanoparticles as energy acceptors into a chemiluminescence resonance energy transfer immunoassay system.  

PubMed

A novel chemiluminescence resonance energy transfer (CRET) system for competitive immunoassay of biomolecules was developed by using novel amorphous carbon nanoparticles (CNPs) prepared from candle soot as energy acceptors. The CNPs were firstly prepared to bind with the antigen (Ag) for obtaining the nanocomposite CNP-Ag, and this obtained CNP-Ag was then reacted with the horseradish peroxidase-labeled antibody (HRP-Ab) to assemble the CRET system. The luminol catalyzed by HRP serving as the energy donor for CNPs triggered the CRET phenomenon between luminol and CNPs, which led to the chemiluminescence signal decrease. Due to the competitive immunoreaction of the target antigen and the CNP-Ag, a part of the CNP-Ag was replaced from the HRP-Ab, and then resulted in a weaker interaction between luminol and CNPs. Thus the competitive immunoreaction led to a higher chemiluminescence emission. This CNP-based CRET system was successfully applied to detect the human IgG as a model analyte, and a linear range of 10-200 ng mL(-1) and a detection limit of 1.9 ng mL(-1) (S/N = 3) were obtained. The results for real sample analysis demonstrated its application potential in some important areas such as clinical diagnosis. PMID:23979821

Wang, Zhenxing; Gao, Hongfei; Fu, Zhifeng

2013-10-15

251

Measurement of hydroxyl radical production in ultrasonic aqueous solutions by a novel chemiluminescence method.  

PubMed

Measurement methods for ultrasonic fields are important for reasons of safety. The investigation of an ultrasonic field can be performed by detecting the yield of hydroxyl radicals resulting from ultrasonic cavitations. In this paper, a novel method is introduced for detecting hydroxyl radicals by a chemiluminescence (CL) reaction of luminol-hydrogen peroxide (H2O2)-K5[Cu(HIO6)2](DPC). The yield of hydroxyl radicals is calculated directly by the relative CL intensity according to the corresponding concentration of H2O2. This proposed CL method makes it possible to perform an in-line and real-time assay of hydroxyl radicals in an ultrasonic aqueous solution. With flow injection (FI) technology, this novel CL reaction is sensitive enough to detect ultra trace amounts of H2O2 with a limit of detection (3sigma) of 4.1 x 10(-11) mol L(-1). The influences of ultrasonic output power and ultrasonic treatment time on the yield of hydroxyl radicals by an ultrasound generator were also studied. The results indicate that the amount of hydroxyl radicals increases with the increase of ultrasonic output power (< or = 15 W mL(-1)). There is a linear relationship between the time of ultrasonic treatment and the yield of H2O2. The ultrasonic field of an ultrasonic cleaning baths has been measured by calculating the yield of hydroxyl radicals. PMID:18289912

Hu, Yufei; Zhang, Zhujun; Yang, Chunyan

2008-01-15

252

Sequential injection chemiluminescence immunoassay for nonionic surfactants by using magnetic microbeads.  

PubMed

A rapid and sensitive immunoassay based on a sequential injection analysis (SIA) using magnetic microbeads for the determination of alkylphenol polyethoxylates (APnEOs) is described. An SIA system was constructed from a syringe pump, a switching valve, a flow-through type immunoreaction cell equipped with a photon counting unit and a neodymium magnet. Magnetic beads, to which an anti-APnEOs monoclonal antibody was immobilized, were used as a solid support in an immunoassay. The introduction, trapping and release of the magnetic beads in and from the immunoreaction cell were controlled by means of a neodymium magnet and adjusting the flow of a carrier solution. The immunoassay was based on an indirect competitive immunoreaction of an anti-APnEOs monoclonal antibody immobilized on the magnetic beads with a sample APnEOs and a horseradish peroxidase (HRP)-labeled APnEOs in the same sample solution, and was based on the subsequent chemiluminscence reaction of HRP on the magnetic microbeads with a luminol solution containing hydrogen peroxide and p-iodophenol. The anti-APnEOs antibody was immobilized on the magnetic microbeads by coupling the antibody with the magnetic beads after activation of a carboxylate moiety on the surface of the magnetic beads that had been coated with a polylactic acid film. The antibody immobilized magnetic beads were introduced in the immunoreaction cell and trapped in it by the neodymium magnet, which was equipped beneath the immunoreaction cell. An APnEOs sample solution containing the HRP-labeled APnEOs at a constant concentration, and a luminol solution containing hydrogen peroxide and p-iodophenol were sequentially introduced into the immunoreaction cell, according to an SIA programmed sequence. Chemiluminescence emission was monitored by means of a photon counting unit located at the upper side of the immunoreaction cell by collecting the emitted light with a lens. A typical sigmoidal calibration curve was obtained, when the logarithm of the concentration of APnEOs was plotted against the chemiluminescence intensity as the number of photons in 100 ms using standard APnEOs sample solutions at various concentrations (0-1000 ppb) under optimum conditions. The lower detection limit defined as IC(80) is ca 10 ppb. The time required for analysis is less than 15 min per a sample. The present method was successfully applied to the determination of APnEOs in river water. PMID:17903471

Zhang, Ruiqi; Nakajima, Hizuru; Soh, Nobuaki; Nakano, Koji; Masadome, Takashi; Nagata, Kazumi; Sakamoto, Kazuhira; Imato, Toshihiko

2007-02-24

253

Chemiluminescence methods for the evaluation of degradative processes in soil organic matter  

NASA Astrophysics Data System (ADS)

Application of chemiluminescent methods for monitoring and quantification of degradation processes of humus substances (HS) in soil organic matter under detrimental environment conditions is described. Single photoelectron counting and imaging techniques of chemiluminescence accompanying degradation reactions of HS as well as quenching of electrochemiluminescence and lucigenin chemiluminescence by degraded HS are applied.

Slawinska, Danuta; Slawinski, Janusz; Gorski, Zbigniew

2004-08-01

254

Recent Advances in Chemiluminescence  

Microsoft Academic Search

This article reviews the development and application of chemiluminescence (CL) published in the literature between January 2004 and October 2006, with regard to instrumentation, systems, applications, and conclusions.

Yingying Su; He Chen; Zhimeng Wang; Yi Lv

2007-01-01

255

Predicted NO2 IR chemiluminescence in the natural atmosphere  

NASA Astrophysics Data System (ADS)

Results from the author's recent theoretical study of NO2 IR Chemiluminescence from the NO + 0 and NO + 03 reactions are employed in a new upper atmospheric NO2 IR radiance model. Predictions are made for altitude dependent and column-integrated NO2 chemiluminescent radiance in four vibrational bands, and comparison is made with previous model predictions.

Adler-Golden, S.

1986-05-01

256

Balancing single- and multi-reference correlation in the chemiluminescent reaction of dioxetanone using the anti-Hermitian contracted Schroedinger equation  

SciTech Connect

Direct computation of energies and two-electron reduced density matrices (2-RDMs) from the anti-Hermitian contracted Schroedinger equation (ACSE) [D. A. Mazziotti, Phys. Rev. Lett. 97, 143002 (2006)], it is shown, recovers both single- and multi-reference electron correlation in the chemiluminescent reaction of dioxetanone especially in the vicinity of the conical intersection where strong correlation is important. Dioxetanone, the light-producing moiety of firefly luciferin, efficiently converts chemical energy into light by accessing its excited-state surface via a conical intersection. Our previous active-space 2-RDM study of dioxetanone [L. Greenman and D. A. Mazziotti, J. Chem. Phys. 133, 164110 (2010)] concluded that correlating 16 electrons in 13 (active) orbitals is required for realistic surfaces without correlating the remaining (inactive) orbitals. In this paper we pursue two complementary goals: (i) to correlate the inactive orbitals in 2-RDMs along dioxetanone's reaction coordinate and compare these results with those from multireference second-order perturbation theory (MRPT2) and (ii) to assess the size of the active space--the number of correlated electrons and orbitals--required by both MRPT2 and ACSE for accurate energies and surfaces. While MRPT2 recovers very different amounts of correlation with (4,4) and (16,13) active spaces, the ACSE obtains a similar amount of correlation energy with either active space. Nevertheless, subtle differences in excitation energies near the conical intersection suggest that the (16,13) active space is necessary to determine both energetic details and properties. Strong electron correlation is further assessed through several RDM-based metrics including (i) total and relative energies, (ii) the von Neumann entropy based on the 1-electron RDM, as well as the (iii) infinity and (iv) squared Frobenius norms based on the cumulant 2-RDM.

Greenman, Loren; Mazziotti, David A. [Department of Chemistry and James Franck Institute, University of Chicago, Chicago, Illinois 60637 (United States)

2011-05-07

257

On the fluorescence of luminol in a silver nanoparticles complex.  

PubMed

The photophysical properties of luminol in a silver nanoparticles complex have been studied by steady-state and time resolved fluorescence spectroscopy. The effect of the serum albumin on the luminol fluorescence in the silver nanoparticles has been also investigated. It was found that the fluorescence quantum yield value of luminol in a silver nanoparticles complex is ? = 0.00407. The decrease of the average fluorescence lifetime value of the luminol in the silver nanoparticles complex was found to be low, = 1.712 ns. The luminol does not bind to the serum albumins in the presence of silver nanoparticles. The formation of a new species of luminol on silver nanoparticles is discussed. The results have influence regarding the use of luminol as an assay for bio-analytical applications. PMID:23463296

Voicescu, Mariana; Ionescu, Sorana

2013-03-06

258

Microchip electrophoresis with chemiluminescence detection for assaying ascorbic acid and amino acids in single cells  

PubMed Central

A method based on microchip electrophoresis (MCE) with chemiluminescence (CL) detection was developed for the determination of ascorbic acid (AA) and amino acids including tryptophan (Trp), glycine (Gly) and alanine (Ala) present in single cells. Cell injection, loading, lysing, electrophoretic separation, and CL detection were integrated onto a simple cross microfluidic chip. A single cell was loaded in the cross intersection by electrophoretic means through applying a set of potentials at the reservoirs. The docked cell was lysed rapidly under a direct electric field. The intracellular contents were MCE separated within 130 s. CL detection was based on the enhancing effects of AA and amino acids on the CL reaction of luminol with K3[Fe(CN)6]. Rat hepatocytes were prepared and analyzed as the test cellular model. The average intracellular contents of AA, Trp, Gly and Ala in single rat hepatocytes were found to be 38.3 fmol, 5.15 fmol, 3.78 fmol and 3.84 fmol (n=12), respectively.

Zhao, Shulin; Huang, Yong

2009-01-01

259

Highly sensitive immunoassay of carcinoembryonic antigen by capillary electrophoresis with gold nanoparticles amplified chemiluminescence detection.  

PubMed

A noncompetitive immunoassay based on gold nanoparticles (AuNPs) amplified capillary electrophoresis (CE) chemiluminescence (CL) detection was developed for the determination of carcinoembryonic antigen (CEA). In this method, citrate-modified AuNPs were conjugated with horseradish peroxidase (HRP) labeled CEA antibody (Ab*), and incubated with limited amount of CEA antigen. CEA-Ab*-AuNPs complex and excess of Ab*-AuNPs were then separated and quantified by CE with CL detection. Highly sensitive CL detection was achieved by means of p-iodophenol (PIP) enhanced luminol-H2O2-HPR CL reaction and AuNPs amplified. Under the optimal conditions, the CE assay was accomplished within 5min. The linear range for CEA detection was 0.05-20ng/mL with a detection limit of 0.034ng/mL (signal/noise=3), which is three orders magnitude lower than that of without AuNPs amplified. The current method was successfully applied for the quantification of CEA in human serum samples. It was demonstrated that the current CE-CL AuNPs amplified noncompetitive immunoassay was sensitive and highly selective. It may serve as a tool for clinical analysis of CEA to assist in the diagnosis of cancer. PMID:23422894

Jiang, Jing; Zhao, Shulin; Huang, Yong; Qin, Guoxin; Ye, Fanggui

2013-01-23

260

An ultrasensitive chemiluminescent method for the tanshinol borneol ester determination and its pharmacokinetic analysis.  

PubMed

Tanshinol borneol ester (DBZ), a chemical combination of danshensu and borneol, is an experimental drug that exhibits efficacious anti-ischemic activity in animal models. In this work, an ultrasensitive chemiluminescence (CL) method for the determination of DBZ was established based on the inhibitory effect of DBZ on the CL signal produced from the reaction between potassium permanganate and luminol in alkaline solution. The CL intensity responded linearly to the concentration of DBZ in the range 2.0 × 10(-10) to 4.0 × 10(-8) g/mL with a detection limit of 7 × 10(-11) g/mL. The relative standard deviation (RSD) was 3.8% for 4.0 × 10(-9) g DBZ (n?=?11). The proposed method showed characteristics of high sensitivity, simple device and quick. In addition, this proposed method had been applied satisfactorily to the analysis of DBZ in blood. The pharmacokinetics of DBZ in rat has also been studied using the CL method. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23681963

Nie, Fei; Bu, Min; Wu, Lihong; Zheng, Jianbin

2013-05-17

261

Chemometrics-assisted simultaneous determination of cobalt(II) and chromium(III) with flow-injection chemiluminescence method  

NASA Astrophysics Data System (ADS)

In this paper, a flow-injection chemiluminescence (CL) system is proposed for simultaneous determination of Co(II) and Cr(III) with partial least squares calibration. This method is based on the fact that both Co(II) and Cr(III) catalyze the luminol-H 2O 2 CL reaction, and that their catalytic activities are significantly different on the same reaction condition. The CL intensity of Co(II) and Cr(III) was measured and recorded at different pH of reaction medium, and the obtained data were processed by the chemometric approach of partial least squares. The experimental calibration set was composed with nine sample solutions using orthogonal calibration design for two component mixtures. The calibration curve was linear over the concentration range of 2 × 10 -7 to 8 × 10 -10 and 2 × 10 -6 to 4 × 10 -9 g/ml for Co(II) and Cr(III), respectively. The proposed method offers the potential advantages of high sensitivity, simplicity and rapidity for Co(II) and Cr(III) determination, and was successfully applied to the simultaneous determination of both analytes in real water sample.

Li, Baoxin; Wang, Dongmei; Lv, Jiagen; Zhang, Zhujun

2006-09-01

262

A validated silver-nanoparticle-enhanced chemiluminescence method for the determination of citalopram in pharmaceutical preparations and human plasma.  

PubMed

A simple and sensitive chemiluminescence (CL) method was developed for the determination of citalopram in pharmaceutical preparations and human plasma. The method is based on the enhancement of the weak CL signal of the luminol-H2 O2 system. It was found that the CL signal arising from the reaction between alkaline luminol and H2 O2 was greatly increased by the addition of silver nanoparticles in the presence of citalopram. Prepared silver nanoparticles (AgNPs) were characterized by UV-visible spectroscopy and transmission electron microscopy (TEM). Various experimental parameters affecting CL intensity were studied and optimized for the determination of citalopram. Under optimized experimental conditions, CL intensity was found to be proportional to the concentration of citalopram in the range 40-2500?ng/mL, with a correlation coefficient of 0.9997. The limit of detection (LOD) and limit of quantification (LOQ) of the devised method were 3.78 and 12.62?ng/mL, respectively. Furthermore, the developed method was found to have excellent reproducibility with a relative standard deviation (RSD) of 3.65% (n?=?7). Potential interference by common excipients was also studied. The method was validated statistically using recovery studies and was successfully applied to the determination of citalopram in the pure form, in pharmaceutical preparations and in spiked human plasma samples. Percentage recoveries were found to range from 97.71 to 101.99% for the pure form, from 97.84 to 102.78% for pharmaceutical preparations and from 95.65 to 100.35% for spiked human plasma. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23754499

Khan, Muhammad Naeem; Jan, Muhammad Rasul; Shah, Jasmin; Lee, Sang Hak

2013-06-11

263

Fast gas chromotography with luminol detection for measurement of nitrogen dioxide and PANs.  

SciTech Connect

Fast capillary gas chromatography has been coupled to a luminol-based chemiluminescence detection system for the rapid monitoring of nitrogen dioxide and peroxyacyl nitrates. A first-generation instrument was described recently (Gaffney et al., 1998). This system is capable of monitoring nitrogen dioxide and peroxyacyl nitrates (PANs; to and including the C4 species) with 1-min time resolution. This is an improvement by a factor of five over gas chromatography methods with electron capture detection. In addition, the luminol method is substantially less expensive than laser fluorescent detection or mass spectroscopic methods. Applications in aircraft-based research have been published electronically and will appear shortly in Environmental Science and Technology (Gaffney et al., 1999a). An improved version of the instrument that has been designed and built makes use of a Hammamatsu photon-counting system. Detection limits of this instrumentation are at the low tens of ppt. The range of the instrument can be adjusted by modifying sampling volumes and detection counting times. A review of past work and of recent application of the instrumentation to field measurements of nitrogen dioxide and PANs is presented. The data clearly indicate that the luminol approach can determine the target species with time resolution of less than 1 min. Examples of applications for estimation of peroxyacetyl radical concentrations and nitrate radical formation rates are also presented. This instrumentation can further be used for evaluation of surfaces for loss of nitrogen dioxide and PANs, phenomena of possible importance for sampling interfaces and chamber wall design. Our high-frequency field data clearly indicate that the ''real world'' is not well mixed and that turbulent mixing and plume-edge chemistries might play an important role in urban- and regional-scale interactions. Dynamic flow systems might be required to evaluate such effects in new-generation chamber studies.

Gaffney, J. S.; Marley, N. A.; Drayton, P. J.

1999-09-30

264

Effect of Platelet-Activating Factor on Human Polymorphonuclear Leukocyte Enhancement of Chemiluminescence and Antibody-Dependent Cellular Cytotoxicity  

Microsoft Academic Search

The effect of platelet-activating factor on human granulocytes was determined by luminol-dependent chemiluminescence (CL), antibody-dependent cellular cytotoxicity (ADCC), and rosette assay. An activation of the respiratory burst could be measured by CL and be shown to depend on the presence of extracellular calcium. This direct stimulation of PMN was proved to be inhibited by oxygen radical scavengers as well as

Horst Mossmann; Uwe Bamberger; Boris A. Velev; Marina Gehrung; Dietrich K. Hammer

265

Tested Demonstrations. A Chemiluminescence Demonstration - Oxalyl Chloride Oxidation.  

ERIC Educational Resources Information Center

|This inexpensive, effective chemiluminescence demonstration requires minimal preparation. It is based on the oxidation of oxalyl chloride by hydrogen peroxide in the presence of an appropriate fluorescent sensitizer. The reaction mechanism is not completely understood. (BB)|

Gilber, George L., Ed.

1979-01-01

266

Chemiluminescence of indole and its derivatives  

NASA Astrophysics Data System (ADS)

The results of studies on chemiluminescence of indole and its derivatives are critically analyzed. It is shown that chemical transformations of indoles lead, depending on the structure and experimental conditions, to various electronically excited products and emission of light. Many reactions considered are used as a basis for highly sensitive methods for detection of indoles in biology, medicine, ecology and forensics.

Vasil'ev, Rostislav F.; Trofimov, A. V.; Tsaplev, Yuri B.

2010-02-01

267

Oscillating chemiluminescence systems: state of the art.  

PubMed

Oscillating chemiluminescence (CL) was reported for the first time about 30 years ago. Since then several systems based on addition of a chemiluminescent reagent to a known oscillator system or based on the light emitting features of one component of the oscillating system, have been described. This information, scattered in the scientific literature, is compiled in the present paper. Several oscillating CL systems, including those based on Belousov-Zhabotinskii and Orban oscillators, or horseradish peroxidase-catalyzed reactions, among others, are critically presented. The application of this type of oscillatory systems is also discussed, in analytical chemistry and for educational purposes. PMID:20354969

Iranifam, Mortaza; Segundo, Marcela A; Santos, João L M; Lima, José L F C; Sorouraddin, Mohammad H

2010-03-30

268

Chemiluminescent Marker Apparatus.  

National Technical Information Service (NTIS)

The patent application relates to a chemiluminescent lighting apparatus for generating an illuminated marker material for delivery to a desired area. Two fluids to be mixed are contained in separate chambers and are separated from a mixing chamber by mean...

R. L. Gerber

1974-01-01

269

Chemiluminescence determination of melatonin and some of its derivatives using potassium permanganate and formaldehyde system  

Microsoft Academic Search

.  It was found that melatonin and its derivatives, such as N-acetyl- 5-methoxytryptamine (MT), N-acetyl-5-hydroxytryptamine (NAS), 5-Methoxytrypt- amine (5-MT), 5-Methoxyindolyl acetic acid (5-MIAA) and N-acetyl-5-methoxy- 6-hydroxytryptamine (6-HMT) would give chemiluminescence in the acidic potassium permanganate solution, and formaldehyde would enhance this chemiluminescent reaction greatly. The optimum conditions for this chemiluminescent reaction were studied in detail by a flow injection system. A

Guo Nan Chen; Fu Xin Huang; Xiao Ping Wu; Zheng Feng Zhao; Jian Ping Duan

2003-01-01

270

Luminol Electrochemiluminescence-Based Biosensor for Total Cholesterol Determination in Natural Samples  

Microsoft Academic Search

A new cholesterol flow injection analysis biosensor is described. It is based on a luminol\\/hydrogen peroxide electrochemiluminescence (ECL) reaction induced by a glassy carbon electrode polarised at + 425 mV vs a Pt pseudo reference. The cholesterol b8543158 sensing layer is based on cholesterol oxidase (COD) immobilised on either UltraBind membrane or Immunodyne membrane. The physicochemical properties of the UltraBind

Christophe A. Marquette; Stéphanie Ravaud; Loïc J. Blum

2000-01-01

271

Peculiarities of luminol- and lucigenin-dependent photon emission from nondiluted human blood  

NASA Astrophysics Data System (ADS)

Comparison of lucigenin- and luminol-dependent chemiluminescence (LC-CL and LM-CL, respectively) in nondiluted healthy donors' blood revealed significant differences in their patterns. LM-CL was low in fresh blood and disappeared after it storage for 3 hours. LC-CL was already high in fresh blood and was steadily increasing with blood storage. Serial dilution of blood with saline after addition of chemiluminescence indicators resulted in elevation of LM-CL, but decrease in LC-CL. LM-CL elevation after the initiation of respiratory burst (RB) in blood with zymosan was observed only in aerated samples and immediately dropped down when air supply to a blood sample was ceased. On the contrary, LM-CL did not depend on air supply to a blood sample for about 30 min. after RB initiation. The results suggest that there are at least two mechanisms for reactive oxygen species production in nondiluted blood. The first one is reflected predominantly by LM-CL. It is activated during RB and uses prevalently oxygen dissolved in cell medium. Another one is reflected predominantly by LC- LM. It does not depend upon initiation of RB in neutrophils, operates in blood constantly, and uses oxygen supplied by erythrocytes. It needs blood integrity for its manifestation.

Voeikov, Vladimir L.; Novikov, Cyrill N.

1997-12-01

272

[Chemiluminescence spectroscopic analysis of homogeneous charge compression ignition combustion processes].  

PubMed

To study the combustion reaction kinetics of homogeneous charge compression ignition (HCCI) under different port injection strategies and intake temperature conditions, the tests were carried out on a modified single-cylinder optical engine using chemiluminescence spectroscopic analysis. The experimental conditions are keeping the fuel mass constant; fueling the n-heptane; controlling speed at 600 r x min(-1) and inlet pressure at 0.1 MPa; controlling inlet temperature at 95 degrees C and 125 degrees C, respectively. The results of chemiluminescence spectrum show that the chemiluminescence is quite faint during low temperature heat release (LTHR), and these bands spectrum originates from formaldehyde (CH2O) chemiluminescence. During the phase of later LTHR-negative temperature coefficient (NTC)-early high temperature heat release (HTHR), these bands spectrum also originates from formaldehyde (CH2O) chemiluminescence. The CO--O* continuum is strong during HTHR, and radicals such as OH, HCO, CH and CH2O appear superimposed on this CO--O* continuum. After the HTHR, the chemiluminescence intensity is quite faint. In comparison to the start of injection (SOI) of -30 degrees ATDC, the chemiluminescence intensity is higher under the SOI = -300 degrees ATDC condition due to the more intense emissions of CO--O* continuum. And more radicals of HCO and OH are formed, which also indicates a more intense combustion reaction. Similarly, more intense CO--O* continuum and more radicals of HCO and OH are emitted under higher intake temperature case. PMID:21137383

Liu, Hai-feng; Yao, Ming-fa; Jin, Chao; Zhang, Peng; Li, Zhe-ming; Zheng, Zun-qing

2010-10-01

273

Study on the proteins-luminol binding by use of luminol as a fluorescence probe  

NASA Astrophysics Data System (ADS)

In this paper, a new mathematical equation of lg(F0 - F)/F = 1/nlg[P] + 1/nlgKa, which was used to obtain interaction parameters (the binding constant Ka and the number of binding sites n) between the protein and the small molecule ligand by using the ligand as a fluorescence (FL) probe, was constructed for the first time. The interaction parameters between myoglobin, catalase, lysozyme, bovine serum albumin (BSA) and luminol were obtained by this equation with luminol used as a FL probe, showing that the binding constants Ka were 8.78 × 105, 4.47 × 105, 4.21 × 104 and 3.95 × 104 respectively, and the number of binding sites n approximately equaled to 1.0 for myoglobin, catalase, and 2.0 for lysozyme, BSA. The interactions of ferritin, ovalbumin, aldolase, chymotrypsinogen and ribonuclease with luminol were also studied by this method. The binding constants Ka were at 104-105 level, and the number of binding sites n mostly approximately equaled to 2.0. The binding ability of luminol to the studied proteins followed the pattern: myoglobin > aldolase > ferritin > ovalbumin > catalase > ribonuclease > lysozyme > BSA > chymotrypsinoge.

He, Xili; Song, Zhenghua

2013-10-01

274

Luminol as a fluorescent acid-base indicator.  

PubMed

The acid and base dissociation constants of luminol are determined at various ionic strengths. The transition interval occurs at pH 7.7-9.0, therefore luminol is a fluorescent indicator for the titration of strong and weak acids and strong bases. Its value as an indicator is established by titrating milk, red wine and cherry juice. PMID:18959899

Erdey, L; Buzás, I; Vigh, K

1966-03-01

275

Concerning Chemiluminescence of Rhodamine C.  

National Technical Information Service (NTIS)

A chemiluminescence method of measuring ozone in the atmosphere is described. Methods of preparing chemiluminescent materials using Reginer's technique involving Rhodamine C are discussed, as well as the luminescence spectrum of this material and results ...

L. G. Bolshakova V. A. Vasiliyeva

1972-01-01

276

A homogeneous chemiluminescent immunoassay method.  

PubMed

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte. PMID:23477541

Akhavan-Tafti, Hashem; Binger, Dean G; Blackwood, John J; Chen, Ying; Creager, Richard S; de Silva, Renuka; Eickholt, Robert A; Gaibor, Jose E; Handley, Richard S; Kapsner, Kenneth P; Lopac, Senja K; Mazelis, Michael E; McLernon, Terri L; Mendoza, James D; Odegaard, Bruce H; Reddy, Sarada G; Salvati, Michael; Schoenfelner, Barry A; Shapir, Nir; Shelly, Katherine R; Todtleben, Jeff C; Wang, Guoping; Xie, Wenhua

2013-03-11

277

Flow-injection chemiluminescence sensor for determination of isoniazid in urine sample based on molecularly imprinted polymer  

NASA Astrophysics Data System (ADS)

In this paper, molecularly imprinted polymer (MIP) of isoniazid is synthesized through thermal radical copolymerization of metharylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) in the presence of isoniazid template molecules. A novel flow injection chemiluminescence sensor for isoniazid determination is developed by packing the isoniazid-MIP into the flow cell as recognition elements. Isoniazid could be selectively adsorbed by the MIPs and the adsorbed isoniazid was sensed by its great enhancing effect on the weak CL reaction between luminol and periodate which were mixed in the flow cell. The enhanced CL intensity is linear in the range 2 × 10 -9 to 2 × 10 -7 g/mL and the detection limit is 7 × 10 -10 g/mL (3 ?) isoniazid with a relative standard deviation 2.8% ( n = 9) for 8 × 10 -8 g/mL. The sensor is reversible and reusable. It has a great improvement in sensitivity and selectivity for CL analysis. As a result, the sensor has been successfully applied to determination of isoniazid in human urine. At the same time, the binding characteristic of the polymer to isoniazid was evaluated by batch method and the dynamic method, respectively.

Xiong, Yan; Zhou, Houjiang; Zhang, Zhujun; He, Deyong; He, Chao

2007-02-01

278

Trace-level detection of atrazine using immuno-chemiluminescence: dipstick and automated flow injection analyses formats.  

PubMed

A sensitive chemiluminescence (CL)-based immunoassay technique based on both dipstick and flow injection analytical formats is reported for the detection of atrazine. In the dipstick-based immunoassay technique, antibody (anti-atrazine) was first immobilized on the nitrocellulose membranes. The dipstick was then treated with atrazine and atrazine-horseradish peroxidase conjugate (atra-HRP) to facilitate the competitive binding. The dipstick was further treated with urea-hydrogen peroxide (U-H202) and luminol to generate photons. The number of photons generated was inversely proportional to the atrazine concentration. In the flow injection analysis (FIA) format, the antibody was immobilized on protein-A sepharose matrix and packed in a glass capillary column, which functioned as an immunoreactor. Competitive binding of antigen and antibody occurred. The CL signals generated during the biochemical reactions were correlated with atrazine concentrations in the analytical samples. By using dipstick technique, it was possible to detect atrazine concentration down to 0.1 ng/mL; with the FIA format, the detection of atrazine was down to 0.01 ng/mL. PMID:20334163

Chouhan, Raghuraj S; Rana, Kumar Vikash S; Suri, C Raman; Thampi, Ravindranathan K; Thakur, Munna S

279

Time-resolved IR chemiluminescence in gas-phase chemical kinetics  

Microsoft Academic Search

The time-resolved IR chemiluminescence method based on the study of the chemiluminescence of vibrationally excited molecules formed in chemical reactions is discussed. The approaches used in the study of elementary reactions and complex processes are systematised. The technique is compared with other methods for the study of gas-phase chemical kinetics. The bibliography includes 76 references.

Evgenii N. Chesnokov; Viktor N. Panfilov

1999-01-01

280

Theoretical study of the correlation between superoxide anion consumption and firefly luciferin chemiluminescence  

NASA Astrophysics Data System (ADS)

This is the first theoretical study of the relationship between superoxide anion and firefly chemiluminescence, in DMSO. Electron transfer reactions between luciferin dianionic/carbanionic/radical species and superoxide were studied in order see if an alternative explanation existed for the consumption of the latter species, without correlating it with a role on luciferin chemiluminescence. Despite the finding that luciferin may indeed inhibit the formation of the superoxide anion, no theoretical evidence was found that showed that this molecule is consumed in a non-chemiluminescence reaction. Therefore, it is concluded that the superoxide anion is indeed related to the firefly luciferin chemiluminescence.

Pinto da Silva, Luís; Esteves da Silva, Joaquim C. G.

2013-07-01

281

A New Enhanced Oscillating Chemiluminescence System with Increased Chemiluminescence Intensity and Oscillation Time  

Microsoft Academic Search

Oscillating chemical reactions are complex systems, involving a large number of chemical species. In an oscillating chemical\\u000a reaction, some species, usually a reaction intermediate, exhibit fluctuation in its concentration. In this report, oscillating\\u000a chemiluminescence produced by the addition of thiosemicarbazide (TSC) to the oscillating system H2O2–KSCN–CuSO4–NaOH was investigated using luminometry method. The effects of complexing agents, triethylenetetramine (TETA) and D-penicillamine,

M. H. Sorouraddin; M. Iranifam; Kaveh Amini

2008-01-01

282

Chemiluminescent optical fiber immunosensor for detecting cholera antitoxin  

NASA Astrophysics Data System (ADS)

A chemiluminescent-based optical fiber immunosensor is developed to detect the presence of jejunal cholera antitoxin IgA immunoglobulins. This was accomplished using optical fiber tips, conjugated with the cholera toxin B subunit. The cholera antitoxin analyte is marked by a secondary antibody labeled with horseradish peroxidase. A photoelectronic setup is designed specifically to monitor the signal. This immunosensor system is shown to be specific, sensitive, and fast to run, without requiring a purification step. The lowest titer detected was 1:1,310,720. When the luminol-containing buffer solution was replaced by air, thus dramatically lowering the index of refraction of the surrounding medium, sensitivity increased and cholera antitoxin was detected at an additional titer dilution at 1:2,621,440.

Marks, Robert S.; Bassis, Effim; Bychenko, Alexei; Levine, Myron M.

1997-12-01

283

Intracellular chemiluminescence activates targeted photodynamic destruction of leukaemic cells.  

PubMed

Photodynamic therapy (PDT) involves a two-stage process. A light-absorbing photosensitiser (Ps) is endocytosed and then stimulated by light, inducing transfer of energy to a cytoplasmic acceptor molecule and the generation of reactive oxygen species that initiate damage to cellular membrane components and cytolysis. The expanded use of PDT in the clinic is hindered by the lack of Ps target-cell specificity and the limited tissue penetration by external light radiation. This study demonstrates that bioconjugates composed of transferrin and haematoporphyrin (Tf-Hp), significantly improve the specificity and efficiency of PDT for erythroleukemic cells by a factor of almost seven-fold. Fluorescence microscopy showed that the conjugates accumulate in intracellular vesicles whereas free Hp was mostly membrane bound. Experiments with cells deliberately exposed to Tf-Hp at luminol induces intracellular chemiluminescence. This strategy was then used to obviate the use of external radiation for Ps activation by incubating the cells with luminol either before or together with Tf-Hp. This novel chemical means of PDT activation induced cytotoxicity in 95% of cells. These combined approaches provide an opportunity to develop broader and more effective applications of PDT. PMID:16819545

Laptev, R; Nisnevitch, M; Siboni, G; Malik, Z; Firer, M A

2006-07-04

284

A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples  

Microsoft Academic Search

A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au-catalysed luminol chemiluminesent system. The method has a wide working range (0.05–10 µg L, r = 0.9914), the limit of detection was determined

Chenlin Hu; Nanqin Gan; Zhike He; Lirong Song

2008-01-01

285

Quasi-simultaneous determination of antioxidative activities against superoxide anion and nitric oxide by a combination of sequential injection analysis and flow injection analysis with chemiluminescence detection  

Microsoft Academic Search

A method that combines sequential injection analysis (SIA), flow injection analysis and chemiluminescence (CL) detection was\\u000a developed for the quasi-simultaneous determination of antioxidative activities against superoxide anion $$\\u000a{\\\\left( {{\\\\text{O}}^{ - }_{2} } \\\\right)}\\u000a$$ and nitric oxide (NO). The antioxidative activity was expressed as the decrease in luminol CL intensity caused by the quenching\\u000a of $${\\\\text{O}}^{ - }_{2} $$

Aoi Miyamoto; Kuniko Nakamura; Naoya Kishikawa; Yoshihito Ohba; Kenichiro Nakashima; Naotaka Kuroda

2007-01-01

286

Synthesis, characterization of dihydrolipoic acid capped gold nanoparticles, and functionalization by the electroluminescent luminol.  

PubMed

The use of gold nanoparticles as biological probes requires the improvement of colloidal stability. Dihydrolipoic acid (DHLA), a dithiol obtained by the reduction of thioctic acid, appears therefore very attractive for the stabilization and the further functionalization of gold nanoparticles because DHLA is characterized by a carboxylic acid group and two thiol functions. The ionizable carboxylic acid groups ensure, for pH > or = 8, the water solubility of DHLA-capped gold (Au@DHLA) nanoparticles, prepared by the Brust protocol, and the stability of the resulting colloid by electrostatic repulsions. Moreover almost all DHLA, adsorbed onto gold, adopts a conformation allowing their immobilization by both sulfur ends. It is proved by sulfur K-edge X-ray absorption near edge structure spectroscopy, which appears as an appropriate tool for determining the chemical form of sulfur atoms present in the organic monolayer. Such a grafting renders the DHLA monolayers more resistant to displacement by dithiothreitol than mercaptoundecanoic acid monolayers. The presence of DHLA on gold particles allows their functionalization by the electroluminescent luminol through amine coupling reactions assisted by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. As a luminol-functionalized particle is nine times as bright as a single luminol molecule, the use of the particles as a biological probe with a lower threshold of detection is envisaged. PMID:15752049

Roux, Stéphane; Garcia, Bruno; Bridot, Jean-Luc; Salomé, Murielle; Marquette, Christophe; Lemelle, Laurence; Gillet, Phillipe; Blum, Loïc; Perriat, Pascal; Tillement, Olivier

2005-03-15

287

Experimental research of photodynamic diagnosis mediated by chemiluminescence reagents  

NASA Astrophysics Data System (ADS)

There are some serious limitations existing in conventional photodynamic diagnosis (PDD), such as autofluorescence disturbance, weak red-band absorption efficacy of traditional photosensitizers and quite short penetration depth of excitation light. In this paper a novel method of PDD mediated by chemiluminescence reagents is presented in fabricated mixed samples. Photodynamic therapy (PDT) is believed to act via singlet oxygen (1O2) generated by energy from photosensitizers. 5-Fluoresceinyl Cypridina Luciferin Analog (FCLA), a chemiluminescence reagent, can react with singlet oxygen and emit chemiluminescence with peak wavelength about at 526 nm. In the experiments, FCLA was chosen as an optical reporter of localization of singlet oxygen produced from photosensitized reaction of Hematoporphyrin Derivative (HpD) excited by 630 nm laser. Experimental results, the measurement of fluorescence spectra and the detection of image from chemiluminescence, show that the mixed HpD and FCLA in biologic tissue mixture excited with the laser of 630 nm can emit detectable light signals.

Wang, Juan; Xing, Da; Tang, Yonghong; He, Yonghong

2000-10-01

288

Chemiluminescence characteristics of N-(4-substituted benzyl)isoluminol.  

PubMed

We synthesized N-(4-substituted benzyl)isoluminol which has 4-bromo-, 4-methyl-, 4-methoxy-, 4-nitrogroups. These compounds produced chemiluminescence by the reaction with the oxidizing agent, potassium hexacyanoferrate and hydrogen peroxide, in an alkaline medium. The chemiluminescence intensities of these compounds were 0.03-4.7 times that of isoluminol. We used Hamett substituent constants as a parameter for the electronic substituent effects. The relationship between the amino-H chemical shift value and the Hamett substituent constants showed a good linear correlation. The relationship between the chemiluminescence intensities and the Hamett substituent constants showed a good linear correlation. The relationship between the fluorescence intensities and the Hamett substituent constants also showed a good linear correlation. These results suggest that the change in the electron density around the amino group strongly influences the fluorescence intensities and corresponding chemiluminescence intensities of these derivatives. PMID:11145162

Todoroki, K; Ohba, Y; Zaitsu, K

2000-12-01

289

Transient emitting species in phosphorus chemiluminescence  

Microsoft Academic Search

The phosphorescence of phosphorus oxidation is the oldest and the best known chemiluminescing reaction, but a definitive spectroscopic study of this classic system has been lacking. In this paper we report the results of an investigation of the oxidation of P4 vapor under atmospheric conditions, with added H2O or D2O vapor. In spectral studies of small horizontal rectangular cross sections

Richard J. VanZee; Ahsan U. Khan

1976-01-01

290

A new enhanced oscillating chemiluminescence system with increased chemiluminescence intensity and oscillation time.  

PubMed

Oscillating chemical reactions are complex systems, involving a large number of chemical species. In an oscillating chemical reaction, some species, usually a reaction intermediate, exhibit fluctuation in its concentration. In this report, oscillating chemiluminescence produced by the addition of thiosemicarbazide (TSC) to the oscillating system H(2)O(2)-KSCN-CuSO(4)-NaOH was investigated using luminometry method. The effects of complexing agents, triethylenetetramine (TETA) and D-penicillamine, on the behavior of the oscillating system were investigated. Moreover, the influence of non-aqueous solvents, dimethyl sulfoxide (DMSO), nitromethane and acetonitrile, was studied. In the presence of solvents with high protophility, the chemiluminescence (CL) intensity was increased (sevenfold), the light emitting and oscillating time was enhanced by threefold. In addition, the effect of presence of non-ionic, cationic, and anionic surfactants was investigated. Non-ionic surfactant increased the intensity of the oscillating CL reaction by 4.5-fold. PMID:18074204

Sorouraddin, M H; Iranifam, M; Amini, Kaveh

2007-12-13

291

BEHAVIOR OF ENHANCED CHEMILUMINESENCE PEROXIDASE-CATALYZED PEROXIDATION OF LUMINOL IN THE SYSTEM OF SURFACTANT{WATER{ORGANIC SOLVENT  

Microsoft Academic Search

This work includes some observations of the chemiluminescent reaction behavior in the system surfactant (Triton X-100 and Aerosol OT) -water-organic solvent (octane and hexane). The HRP-chemiluminescent activity was detected without the surfactant or using low surfactant concentration (less than 0.01 M). The greatest activity was ob- served applying the surfactant at 0.001 M. It was demonstrated that in the system

A. D. Ilyina; B. J. E. Mauricio; S. I. P. Sifuentes; H. J. L. Mart; E. S. Bogatcheva; G. J. Romero; M. J. Rodr

292

40 CFR 1065.270 - Chemiluminescent detector.  

Code of Federal Regulations, 2013 CFR

...2013-07-01 false Chemiluminescent detector. 1065.270 Section 1065.270...Measurements § 1065.270 Chemiluminescent detector. (a) Application. You may use a chemiluminescent detector (CLD) to measure NOX...

2013-07-01

293

Sensitive competitive flow injection chemiluminescence immunoassay for IgG using gold nanoparticle as label  

NASA Astrophysics Data System (ADS)

A sensitive competitive flow injection chemiluminescence (CL-FIA) immunoassay for immunoglobulin G (IgG) was developed using gold nanoparticle as CL label. In the configuration, anti-IgG antibody was immobilized on a glass capillary column surface by 3-(aminopropyl)-triethoxysilane and glutaraldehyde to form immunoaffinity column. Analyte IgG and gold nanoparticle labeled IgG were passed through the immunoaffinity column mounted in a flow system and competed for the surface-confined anti-IgG antibody. CL emission was generated from the reaction between luminol and hydrogen peroxide in the presence of Au (III), generated from chemically oxidative dissolution of gold nanoparticle by an injection of 0.10 mol L -1 HCl-0.10 mol L -1 NaCl solution containing 0.10 mmol L -1 Br 2. The concentration of analyte IgG was inversely related to the amount of bound gold nanoparticle labeled IgG and the CL intensity was linear with the concentration of analyte IgG from 1.0 ng mL -1 to 40 ng mL -1 with a detection limit of 5.2 × 10 -10 g mL -1. The whole assay time including the injections and washing steps was only 30 min for one sample, which was competitive with CL immunoassays based on a gold nanoparticle label and magnetic separation. This work demonstrates that the CL immunoassay incorporation of nanoparticle label and flow injection is promising for clinical assay with sensitivity and high-speed.

Qi, Honglan; Shangguan, Li; Liang, Lin; Ling, Chen; Gao, Qiang; Zhang, Chengxiao

2011-11-01

294

Sensitive and selective determination of glucose in human serum and urine based on the peroxyoxalate chemiluminescence reaction of a new Fluorophore  

NASA Astrophysics Data System (ADS)

A novel method for simple and sensitive determination of glucose based on the peroxyoxalate chemiluminescence (PO-CL) detection of enzymatically generated H 2O 2 was investigated. Influence of various experimental parameters on glucose sensing, including the action time of the enzyme, solution pH, interferents and the concentration of CL reagents was investigated. Under the optimum condition, the linear response range of glucose was found to be 2.50 × 10 -6 to 1.75 × 10 -4 mol/L, and the detection limit (defined as the concentration that could be detected at the signal-to-noise ratio of 3) was 1.10 × 10 -6 mol/L. The present method has been used to determine the glucose concentrations in real serum and urine samples with satisfactory results.

Zargoosh, Kiomars; Shamsipur, Mojtaba; Qandalee, Mohammad; Piltan, Mohammad; Moradi, Loghman

2011-10-01

295

Quantitative assessment of rabbit alveolar macrophage function by chemiluminescence  

SciTech Connect

Rabbit alveolar macrophages (RAM) were cultured for 24 hr with concentrations ranging from 3 to 12 ..mu..g/ml of vanadium oxide (V/sub 2/O/sub 5/), a known cytotoxic agent, or with high-molecular-weight organic by-products from coal gasification processes. After culture the cells were harvested and tested for functional capacity using three types of indicators: (1) luminol-amplified chemiluminescence (CL), which quantitatively detects photon emission due to respiratory burst activity measured in a newly designed instrument with standardized reagents; (2) the reduction of nitro blue tetrazolium-saturated polyacrylamide beads, a semiquantitative measure of respiratory burst activity; and (3) phagocytic efficiency, defined as percentage of cells incorporating immunoglobulin-coated polyacrylamide beads. Chemiluminescence declined linearly with increasing concentrations of V/sub 2/O/sub 5/ over the dose range tested. Dye reduction and phagocytic efficiency similarly decreased with increasing V/sub 2/O/sub 5/ concentration, but were less sensitive indicators of functional impairment than CL as measured by the amount required to reduce the response to 50% of untreated cells. The effect of coal gasification condensates on RAM function varied, but in general these test also indicated that the CL response was the most sensitive indicator.

Brennan, P.C.; Kirchner, F.R.

1985-08-01

296

Quality Control of Reactive Oxygen Species Measurement by Luminol-Dependent Chemiluminescence Assay  

Microsoft Academic Search

A total of 28 donor semen samples were used to eval- uate the characteristics of laboratory variability in measuring reactive oxygen species (ROS). The objectives of this study were to assess the interassay (same sample observed on different days by the same observers) variability; interdonor, intraobserver (replications of the same sample on the same day) variability; and interobserver (multiple observers

HIROSHI KOBAYASHI; ENRIQUE GIL-GUZMAN; AYMAN M. MAHRAN; RAKESH K. SHARMA; DAVID R. NELSON; ANTHONY J. THOMAS JR; ASHOK AGARWAL

297

Capillary gas chromatographic analysis of nitrogen dioxide and pans with luminol chemiluminescent detection  

Microsoft Academic Search

Peroxyacyl nitrates (PANs) and nitrogen dioxide are important atmospheric air pollutants in the troposphere. These atmospheric nitrogen species are strongly coupled chemically by a clearly temperature-dependent equilibrium in the troposphere. A chemical method that can measure both nitrogen dioxide and PANs rapidly and with sub-part-per-billion detection is described that is based upon a modified luminoldetection system coupled to a capillary

J. S. Gaffney; R. M. Bornick; Y.-H. Chen; N. A. Marley

1998-01-01

298

Differential stimulation of luminol-enhanced chemiluminescence (CL) and arachidonic acid metabolism in rat peritoneal neutrophils  

SciTech Connect

Phorbol 12-myristate, 13-acetate (PMA) induced the production of radical oxygen species (ROS) from rat peritoneal neutrophils as assessed by CL. ROS generation occurred in a time- (maximum at 13.5 min) and dose- (concentration range of 1.7-498 nM) related fashion. However, 166 nM PMA did not induce either cyclooxygenase (CO) or lipoxygenase (LPO) product formation by 20 min post-stimulation. Conversely, A23187, at concentrations between 0.1 and 10 ..mu..M, stimulated both pathways of arachidonic acid metabolism, but had little or no effect upon ROS production. When suboptimal concentrations of PMA (5.5 nM) and A23187 (0.1-1 ..mu..M) were coincubated with the neutrophils, a synergistic ROS response was elicited. However, arachidonic acid metabolism in the presence of PMA was unchanged relative to A12187 alone. Nordihydroguaiaretic acid (NDGA) inhibited both PMA-induced CL (IC/sub 50/ = 0.9 ..mu..M) and A23187-induced arachidonic acid metabolism (IC/sub 50/ = 1.7 ..mu..M and 6.0 ..mu..M for LPO and CO, respectively). The mixed LPO-CO inhibitor, BW755C, behaved in a qualitatively similar manner to NDGA, whereas the CO inhibitors, indomethacin, piroxicam and naproxen had no inhibitory effect on ROS generation at concentrations as high as 100 ..mu..M. These results suggest that NDGA and BW755C may inhibit CL and arachidonic acid metabolism by distinct mechanisms in rat neutrophils.

Sturm, R.J.; Adams, L.M.; Cullinan, C.A.; Berkenkopf, J.W.; Weichman, B.M.

1986-03-05

299

Metabolism and disposition of luminol in the rat.  

PubMed

1. The metabolism and disposition of Luminol (LMN, 3-aminophthalhydrazide), a widely used forensic and laboratory reagent that chemiluminesses upon oxidation, was determined as part of its overall toxicological characterization. 2. Radiolabelled LMN was well absorbed, metabolized and excreted following p.o. administration of a range of doses. About 90% of the total dose was recovered within 24 h of administration in urine in the form of two metabolites identified as LMN N8-glucuronide and LMN N8-sulphamic acid. 3-Aminophthalic acid, the oxidative product of LMN in the light-emitting reaction, was apparently not formed in vivo. 3. Metabolism and disposition of an i.v. administered dose was similar to that following gavage. Little or no LMN-derived radioactivity was present in tissue within 12 h post-dosing. Excretion of radioactivity in bile following i.v. injection was minimal (approximately 8% of the total dose in 6 h) and consisted of the same urinary-excreted glucuronide and sulphate conjugates. 4. LMN was not absorbed dermally in rat, potentially a major route of exposure to human. If the fate of LMN is similar between species, this compound should have little potential for either dermal absorption, bioaccumulation in tissues following other routes of exposure or chronic toxicity in humans. PMID:10752641

Sanders, J M; Chen, L J; Burka, L T; Matthews, H B

2000-03-01

300

Sensitive and selective chemiluminescence assay for hydrogen peroxide in exhaled breath condensate using nanoparticle-based catalysis  

NASA Astrophysics Data System (ADS)

The catalytic properties of cubiform Co3O4 nanoparticles, ?-Fe2O3 nanorods, and NiO nanoparticles were studied using both microarray method and FI-CL method. These nanoarticles exhibit high specific catalytic effects on the chemiluminescence (CL) reaction of the luminol-H2O2 system in alkaline solution compared with other common catalysts. A reaction mechanism is described. It provides new insights into the application of nanoparticle materials. The CL method based on the use of the Co3O4 nanoparticles is ultrasensitive and particularly selective. Therefore, it was applied to the analysis of H2O2 which can be determined in the concentration range from 1.0 nM to 1000 nM, with a detection limit of 0.3 nM. The relative standard deviation is 2.1% at 0.1 ?M of H2O2 (for n = 11). The method was successfully applied to the determination of trace quantities of H2O2 in exhaled breath condensate (EBC) where it is a mediator of oxidative stress and a promising biomarker for diagnosing. The assay requires a small sample and no incubation time, and has an analytical runtime of <1 min. It is timesaving and suitable for larger studies. The levels of H2O2 in EBC are found to be elevated in healthy subjects (average = 0.54 nM), rheum subjects (average = 0.24 nM), and feverish subjects (average = 0.16 nM). Our data suggested that the average H2O2 concentration of EBC from feverish subjects was significantly higher than healthy subjects and rheumatic subjects.

Li, Xiaohua; Zhang, Zhujun; Tao, Liang; Gao, Miao

2013-04-01

301

Luminol-based bioluminescence imaging of mouse mammary tumors.  

PubMed

Polymorphonuclear neutrophils (PMNs) are the most abundant circulating blood leukocytes. They are part of the innate immune system and provide a first line of defense by migrating toward areas of inflammation in response to chemical signals released from the site. Some solid tumors, such as breast cancer, also cause recruitment and activation of PMNs and release of myeloperoxidase. In this study, we demonstrate that administration of luminol to mice that have been transplanted with 4T1 mammary tumor cells permits the detection of myeloperoxidase activity, and consequently, the location of the tumor. Luminol allowed detection of activated PMNs only two days after cancer cell transplantation, even though tumors were not yet palpable. In conclusion, luminol-bioluminescence imaging (BLI) can provide a pathway towards detection of solid tumors at an early stage in preclinical tumor models. PMID:24077442

Alshetaiwi, Hamad S; Balivada, Sivasai; Shrestha, Tej B; Pyle, Marla; Basel, Matthew T; Bossmann, Stefan H; Troyer, Deryl L

2013-09-08

302

Chemiluminescence in the Condensed Phase: Measurements of Rates and Yields of Excited State Formation.  

National Technical Information Service (NTIS)

The report discusses the techniques for studying chemiluminescence quantitatively in condensed phases. The main emphasis is on measuring quantum yields, absolute light production, and the relationship between chemical reaction rates and light emission. Ch...

C. A. Heller H. P. Richter

1972-01-01

303

Determination Co 2+ in vitamin B 12 based on enhancement of 2-(4-substituted-phenyl)-4,5-di(2-furyl) imidazole and H 2O 2 chemiluminescence reaction  

NASA Astrophysics Data System (ADS)

In this paper, three kinds of imidazole derivatives, 2-(4-methylphenyl)-4,5-di(2-furyl) imidazole (MDFI), 2-(4-nitrophenyl)-4,5-di(2-furyl) imidazole (NDFI), and 2-(4-tert-butylphenyl)-4,5-di(2-furyl) imidazole (t-BDFI) were synthesized. In an alkaline medium, the chemiluminescence (CL) reaction of imidazole derivatives with H 2O 2 has been investigated. It was also found that MDFI/H 2O 2 and t-BDFI/H 2O 2 systems gave strong CL. When Co 2+ was added into the two CL systems, the CL intensity was remarkably enhanced. In the optimum conditions, the CL intensity is linearly related to the logarithm of concentration of Co 2+. The linear ranges are 5 × 10 -9-2.5 × 10 -7 mol/L for MDFI/H 2O 2 system and 5 × 10 -9-2.5 × 10 -7 mol/L for t-BDFI/H 2O 2 system, and the corresponding detection limits are 1.2 × 10 -9 mol/L and 1.1 × 10 -9 mol/L, respectively. The method was applied to the determination of Co 2+ in vitamin B 12 injection. Furthermore, the CL mechanism was also discussed.

Han, Lu; Zhang, Yumin; Kang, Jing; Tang, Jieli; Zhang, Yihua

2011-11-01

304

Phagocytic and chemiluminescent responses of mouse peritoneal macrophages to living and killed Salmonella typhimurium and other bacteria  

SciTech Connect

In the presence of luminol, resident as well as thioglycolate-induced and immunized macrophages emitted chemiluminescence more efficiently when the cells were exposed to living Salmonella typhimurium than when they were exposed to the same bacterium killed by ultraviolet light or heat. This phenomenon was observed whether or not the bacterium was opsonized. The different response to living and killed bacteria was also found with Escherichia coli, Pseudomonas aeruginosa, Proteus morganii, and Enterobacter aerogenes, but not with Shigella sonnei, Klebsiella pneumoniae, and Propionibacterium acnes. The results suggest that macrophages respond better to living, motile bacteria than to nonmotile or killed bacteria. The experimental results obtained with motility mutants of S. typhimurium, E. coli, and P. aeruginosa confirm that macrophages exposed to the motile bacteria emit chemiluminescence more efficiently and ingest the motile bacteria at a much faster rate than the nonmotile bacteria.

Tomita, T.; Blumenstock, E.; Kanegasaki, S.

1981-06-01

305

Detection of nucleic acid hybrids by prolonged chemiluminescence  

SciTech Connect

A method for determining a particular single stranded polynucleotide sequence in a test medium, comprising the steps of: (a) immobilizing on a solid support single stranded nucleic acids in the test medium, (b) contacting the immobilized nucleic acids with a polynucleotide probe having a base sequence substantially complementary to the sequence to be determined and the contacting being under conditions favorable to hybridization between the probe and the sequence to be determined, wherein the probe is labeled with a chemiluminescence enhancer, (c) separating the immobilized hybrids from the unhybridized probe, (d) initiating a chemiluminescent reaction by contacting the separated, labeled, immobilized hybrids with an oxidant, a 2.3-dihydro-1,4-phthalazinedione chemiluminescence precursor, and a peroxidase enzyme, (e) detecting the resulting light emission, and (f) relating the amount of emitted light to the amount of the single stranded polynucleotide sequence.

Dattagupta, N.; Clemens, A.H.

1988-12-27

306

Chemiluminescent Monitor for Vinyl Chloride.  

National Technical Information Service (NTIS)

A monitor for vinyl chloride monomer (VCM) in ambient air was constructed using commercially available components of a gas chromatograph (GC) coupled with a chemiluminescence ozone analyzer slightly modified to make it suitable for use as a GC detector. T...

M. W. Greene S. G. Riccio W. D. Dencker R. I. Wilson

1978-01-01

307

Chemiluminescence switching on peroxidase-like Fe3O4 nanoparticles for selective detection and simultaneous determination of various pesticides.  

PubMed

To achieve selectivity in direct chemiluminescence (CL) detection is very significant and a great challenge as well. Here, we report a novel concept of developing intrinsically selective CL switching at the surface of Fe(3)O(4) nanoparticles for the sensitive detection and simultaneous determination of various pesticides. Fe(3)O(4) nanoparticles have peroxidase-like catalytic activity and catalyze the decomposition of dissolved oxygen to generate superoxide anions, so that the CL intensity of luminol was amplified by at least 20 times. The CL signals can be quenched by the addition of ethanol because ethanol readily reacts with superoxide anions as a radical scavenger. However, the quenching effect can be inhibited through the specific binding of target molecules on Fe(3)O(4) nanoparticles, leading to CL "turn-on" in the presence of ethanol. The novel CL "switching-on" concept demonstrated unique advantages in the detection of pesticide residues. Using the surface coordinative reactions, nonredox pesticide ethoprophos were sensitively detected with a detection limit of 0.1 nM and had a very wide detection range of 0.1 nM to 100 ?M. More importantly, the selectivity of CL switching is tunable through the special surface modification of Fe(3)O(4) nanoparticles, and these Fe(3)O(4) nanoparticles with different surface groups can generate unique CL response pattern for the simultaneous determination of various pesticides. Meanwhile, the superparamagnetic properties of Fe(3)O(4) nanoparticles provide a simple magnetic separation approach to attain interference-free measurement for real detection. The very facile and versatile strategy reported here should open a new window to exploration of selective CL molecular switching and application of magnetic nanoparticles for chemo/biodetection. PMID:23025448

Guan, Guijian; Yang, Liang; Mei, Qingsong; Zhang, Kui; Zhang, Zhongping; Han, Ming-Yong

2012-10-17

308

Antioxidant Efficacy on Human Skin in vivo Investigated by UVA-Induced Chemiluminescence Decay Analysis via Induced Chemiluminescence of Human Skin  

Microsoft Academic Search

Background\\/Aims: Exogenous factors (e.g. physical: UV irradiation; or chemical: hydrogen peroxide) and endogenous metabolic processes (e.g. cellular respiration, oxidative burst, etc.) generate oxidative stress in living tissues which are in balance with enzymatic antioxidative systems and ingested antioxidants under normal conditions. These complex biological reactions are accompanied by chemiluminescence (ultraweak photon emission). However, knowledge about the chemiluminescence decay characteristics of

A. Jain; I. Rieger; M. Rohr; A. Schrader

2010-01-01

309

A Study of Prevention of Spontaneous Luminescence of Luminol.  

National Technical Information Service (NTIS)

Two methods are conceivable in preventing the spontaneous luminescence of a luminol reagent. One is to reduce the alkalinity of sodium carbonate by adding some reagents of weak acidity. The other method is to add reagents that suppress the decomposition o...

M. Arai

1964-01-01

310

Detection of synthetic corticosteroids in bovine urine by chemiluminescence high-performance liquid chromatography.  

PubMed

The development of a black market of chemical cocktails for illegal growth promotion in food-producing animals includes substances that are potentially dangerous for human health, such as synthetic corticosteroids. The potential presence of these residues in food makes it necessary to develop rapid and sensitive analytical methodologies to detect such substances, preferably in live animals before they arrive at the market. A chemiluminescence (CL) detection method for the determination of four synthetic corticosteroids (prednisolone, betamethasone, dexamethasone and flumethasone) in bovine urine has been developed. The proposed system, which does not need any derivatization procedure, offers an easy method well suited for routine research. Urine samples were homogenized with methanol:water (50:50, v/v) and centrifuged. The upper layer was collected and Strata X cartridges were used for cleaning up. The purified residues were evaporated to dryness and then redissolved in the mobile phase. Analysis of the extracts was performed using high-performance liquid chromatography with chemiluminescence detection, employing luminol as the CL reagent. The recovery curves, obtained at four spiking levels (different for each corticosteroid), showed that recoveries of at least 70% could be obtained for urine. The chemiluminescence detection procedure afforded satisfactory results with respect to sensitivity and the LOD and LOQ, taken as the first point of the regression curve, ranged from 4 ppb to 65 ppb. The maximum mean RSD was below 13% and below 15% for intra- and inter-day assay, respectively, in all cases. PMID:15924320

Vázquez, B I; Feás, X; Lolo, M; Fente, C A; Franco, C M; Cepeda, A

311

Chemiluminescence of pulmonary macrophages from patients with inactive sarcoidosis, in different radiological stages.  

PubMed

Luminol-amplified chemiluminescence was generated by alveolar macrophages, harvested from the bronchoalveolar lavages of 16 patients with different radiological stages of non-invasive (asymptomatic) sarcoidosis. None of the patients received any steroid therapy during this study. The mean duration of the disease in these patients was 8 months, with a duration time range of 6-14 months. Six patients were in radiological stage 1, five in radiological stage 2 and five in radiological stage 3. Alveolar macrophages from bronchoalveolar lavages of eight healthy non-smoking volunteers were used as controls. All alveolar macrophages were stimulated by phorbol myristate acetate. A significant decrease was recorded in the intensity of chemiluminescence generated by the phorbol-ester-stimulated alveolar macrophages obtained from patients with sarcoidosis of radiological stages 1 and 2, as compared to the cells collected from healthy individuals (controls). No decrease was recorded in the chemiluminescence generated by stimulated alveolar macrophages collected from patients with radiological stage 3, or from unstimulated alveolar macrophages of any patient. These results provide us with an indicative tool, which might enable us to differentiate, on a functional basis, between the activities of alveolar macrophages in non-active sarcoidosis. PMID:12687630

Czuba, Zenon P; Zydowicz, Grzegorz; Ziora, Dariusz; Cieslicki, Jan; Oklek, Kazimierz; Beck, Brygida; Shani, Jashovam; Krol, Wojciech

312

Oscillating chemiluminescence with thiosemicarbazide in a batch reactor.  

PubMed

Oscillating chemical reactions are complex systems involving a large number of chemical species. In oscillating chemical reactions, some species, usually reaction intermediates, exhibit fluctuations in their concentration. In this report, a novel slowly-damped oscillating chemiluminescence produced by the addition of thiosemicarbazide (TSC) to the oscillating system H2O2-KSCN-CuSO4-NaOH was investigated. Narrow and slightly asymmetric light pulses of 1.5 s half-width are emitted at 440 nm, with an oscillation period of 22-363 s, an induction period of 9-397 s and an emitted light time of 700-1500 s, depending on reagent concentrations. In this study the dependence of the induction period and the oscillation period on the reagent concentrations was investigated and both parameters were plotted with respect to reagent concentrations. Copper concentration showed a significant effect on the oscillation period. A possible mechanism for the oscillating chemiluminescence reaction is discussed. PMID:18452144

Sorouraddin, M H; Iranifam, M

313

Short-Time-Response measurements of nitrogen dioxide and peroxyacetyl nitrate by fast capillary gas chromatography with luminol detection.  

National Technical Information Service (NTIS)

The interaction of hydrocarbons and nitrogen oxides in sunlight to produce photochemical smog has been well studied over the years. In the past, the workhorse for the measurement of NO(sub 2)and NO was the chemiluminescent reaction with ozone. This method...

N. A. Marley J. S. Gaffney P. J. Drayton

2000-01-01

314

Theoretical prediction of the photoinduced chemiluminescence of pesticides.  

PubMed

Although it is relatively easy to find chemiluminescent (CL) molecules working on the field of direct liquid phase (especially employing strong oxidants), the molecules found as chemiluminescent are normally very weak CL compounds for developing suitable analytical CL-procedures. Therefore, it is mandatory to develop new strategies to enhance in a simple way the native chemiluminescence of such a compounds, and even to increase the number of compounds to be determined by direct chemiluminescence. Photoinduced chemiluminescence (Ph-CL) results in a simple and easily on-line accessible strategy to solve these disadvantages. In the present paper, molecular connectivity, a topological method which allows an unique mathematical characterization of molecular structures by the so-named topological descriptors and their correlation with physical, chemical and biological properties of molecules was applied to predict the Ph-CL in liquid phase. Molecular connectivity calculations and discriminant analysis was applied to 72 pesticides for which either a Ph-CL or non Ph-CL behaviour was observed in an experimental screening. The screening test is based on the on-line photodegradation of pesticides by using an automated multicommutation based flow asssembly provided with a photoreactor consisting of 150 cm x 0.8mm PTFE tubing helically coiled around a 20 W low-pressure mercury lamp. Photodegraded pesticides are detected by direct chemiluminescence of the resulting photo-fragments and their subsequent reaction with potassium permanganate in sulfuric acid medium as oxidant. The screening comprised pesticides with different molecular structures and relevant members of the most important families of pesticides were tested (oxime carbamates, sulfonylcarbamates, thiocarbamates, 1,3,5-triazines, organophosphorous, hydroxybenzonitrile, sulfonylureas, phosphonic acid derivatives, imidazolinones, carboxamides, aryloxyalkanoic acids, 1,2,4-triazinones, etc.). The theoretical predictions agree with the empirical results obtained by means of the screening test performed in the multicommutation flow-assembly. PMID:19071628

Ricart, I Sahuquillo; Antón-Fos, G M; Duart, M J; Mateo, J V García; Zamora, L Lahuerta; Calatayud, J Martínez

2006-12-08

315

Recent Progress in Chemiluminescence for Gas Analysis  

Microsoft Academic Search

Chemiluminescence is frequently used as a powerful analytical tool for gas analysis. In this mini-review with 102 references, we summarize the recent advances in chemiluminescence-based analytical methodologies and their application in gas\\/volatile species analysis, mainly including applications of ozone-induced chemiluminescence, cataluminescence-based gas sensors and arrays, and dielectric barrier discharge–induced chemiluminescence for gas analysis. Efforts in the innovation of the methodologies,

Lichun Zhang; Jing Hu; Yi Lv; Xiandeng Hou

2010-01-01

316

Stimulus specific effect of ibuprofen on chemiluminescence of sheep neutrophils  

SciTech Connect

The authors have shown that pretreatment with ibuprofen inhibits free radical release from complement stimulated neutrophils. To further examine the effect of ibuprofen on neutrophil free radical release, they stimulated neutrophils with the synthetic peptide, FMLP, phorbol myristate acetate (PMA), or zymosan-activated plasma (ZAP). Pure (>95%), viable (>95%) sheep neutrophils (2 x 10/sup 6/) were placed in HEPES buffer, luminol, drug or vehicle and stimulated in the luminometer with one of the stimuli. The chemiluminescence (CL) response was recorded and the drug treated samples were compared to vehicle treated controls. Ibuprofen had a dose dependent effect on CL in ZAP stimulated neutrophils. At the highest dose (10/sup -2/M) these cells produced only 37 +/- 7% of the CL response observed in the control cells. In contrast, at the same dose, ibuprofen did not significantly attenuate CL seen in FMLP stimulated cells, with these cells producing 79 +/- 7% of the control cells; nor did ibuprofen effect PMA stimulated CL, as these cells produced a CL response that was 85 +/- 8% of the control cells. Ibuprofen appears to have a stimulus specific effect on free radical release in activated neutrophils. It is also apparent that ibuprofen inhibits complement stimulated free radical release by some mechanism independent of its cyclooxygenase inhibitory effect.

Tahamont, M.V.; Margiotta, M.; Gee, M.H.

1986-03-05

317

[Chemiluminescent determination of iron, copper and cobalt in the serum].  

PubMed

Iron in the presence of diethylenetriamine, an activator, is a catalyst of the chemiluminescent reaction between 4-diethylaminophthalhydraside and hydrogen peroxide. The following conditions are optimal for iron content determination: concentration of 4-diethylaminophthalhydraside - (4-5) X 10(-5) M, H2O2 - 1 X 10(-3) M, diethylenetriamine - 5 X 10(-4) M; pH 10-11. Chemoluminescent reaction between 4-diethylaminophthalhydrazide and hydrogen peroxide is catalyzed also by copper and cobalt ions. This reaction is studied for the possibility to be used to determine iron, copper and cobalt amount in solution without their preliminary separation. A highly sensitive procedure is developed for chemiluminescent determination of these metals concentration in the blood serum with detection limit for iron 1.0, for copper - 0.1 and for cobalt - 0.05 ng/ml. PMID:4024301

Zaporozhets, O A; Dubovenko, L I; Piatnitski?, I V

318

Chemiluminescence of cerium(IV)–rhodamine 6G–phenolic compound system  

Microsoft Academic Search

The oxidation reaction between cerium(IV) and rhodamine 6G in sulfuric acid medium underwent weak chemiluminescence (CL). The effects of 53 organic compounds of interest on cerium(IV)–rhodamine 6G chemiluminescence were investigated by a flow injection procedure, and 32 phenolic compounds were found to enhance CL. Phenolic compounds mainly include phenols, polyphenols, phenolic acids, hydroxycinnamic acids and flavonoids. The correlation between CL

Hua Cui; Qunlin Zhang; Aung Myint; Xuewu Ge; Lijuan Liu

2006-01-01

319

Chemiluminescence flow-injection analysis of captopril applying a sensitized rhodamine 6G method  

Microsoft Academic Search

A flow-injection analysis with chemiluminescence detection is described for the determination of captopril based on the photochemical reaction with cerium(IV) in sulphuric acid medium yielding a strong chemiluminescent signal which can be sensitized by some fluorescers. The proposed procedure has a linear application range of 1 × 10?6–2 × 10?4 M (r = 0.997) with a detection limit of 2

Z. D. Zhang; W. R. G. Baeyens; X. R. Zhang; G. Van der Weken

1996-01-01

320

Developments and Applications of Electrogenerated Chemiluminescence Sensors Based on Micro- and Nanomaterials  

PubMed Central

A variety of recent developments and applications of electrogenerated chemiluminescence (ECL) for sensors are described. While tris(2,2?-bipyridyl)-ruthenium(II) and luminol have dominated and continue to pervade the field of ECL-based sensors, recent work has focused on use of these lumophores with micro- and nanomaterials. It has also extended to inherently luminescent nanomaterials, such as quantum dots. Sensor configurations including microelectrode arrays and microfluidics are reviewed and, with the recent trend toward increased use of nanomaterials, special attention has been given to sensors which include thin films, nanoparticles and nanotubes. Applications of ECL labels and examples of label-free sensing that incorporate nanomaterials are also discussed.

Hazelton, Sandra G.; Zheng, Xingwang; Zhao, Julia Xiaojun; Pierce, David T.

2008-01-01

321

Influence of the nanoscale structure of gold thin films upon peroxidase-induced chemiluminescence  

NASA Astrophysics Data System (ADS)

Gold thin films with different nanoscaled roughness were elaborated by a pulsed-laser deposition technique in order to evaluate their ability to form biochip substrates. The crystal structure, microstructure, and optical absorption were investigated by x-ray diffraction, atomic force microscopy, and ultraviolet-visible absorption spectrum. Controlling the temperature of the substrate during the deposition process allows us to obtain samples with different roughness and grain sizes. The temperature can then be adjusted to elaborate thin films, which have either the optical behavior of bulk metal or that of individual clusters. This optical behavior strongly influences the chemiluminescence at 425 nm of luminol brought to the vicinity of peroxidase supported by biomolecules physi- or chemisorbed on the films. In particular, the signal intensity increases of almost one order of magnitude when the film presents a significant surface resonance plasmon.

Lu, Guowei; Cheng, Bolin; Shen, Hong; Chen, Zhenghao; Yang, Guozhen; Marquette, Christophe A.; Blum, Loic Jean; Tillement, Olivier; Roux, Stéphane; Ledoux, Gilles; Descamps, Armel; Perriat, Pascal

2006-01-01

322

Spatial and temporal control of microwave triggered chemiluminescence: a protein detection platform.  

PubMed

We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now "trigger" chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in "on-demand" photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed "bow-tie" structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. We believe analytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefit from this new platform technology. PMID:17696497

Previte, Michael J R; Aslan, Kadir; Geddes, Chris D

2007-08-16

323

Flow-injection chemiluminescence determination of gentamicin: optimization by central composite design.  

PubMed

A simple and sensitive flow-injection chemiluminescence (CL) method has been developed for the determination of gentamicin sulfate. The method is based on the inhibitory effect of gentamicin on the CL emission accompanying oxidation of luminol by H2 O2 in an alkaline medium in the presence of Cu(II) as a catalyst. Inhibition was caused by the formation of a strong complex between analyte and the catalyst. Experimental variables, including the concentrations of luminol (µmol/L), H2 O2 (mol/L), Cu(II) (mol/L) and NaOH (mol/L), were optimized using a central composite design. Under optimum conditions, the plot of CL intensity versus gentamicin concentration was found to have two linear ranges. One range was at low concentrations from 1.0 to 10.0?mg/L and the other was from 10.0 to 30.0?mg/L. Precision was calculated by analyzing samples containing 5.0?mg/L gentamicin (n?=?11) and the relative standard deviation (RSD) was 1.7%. Also, a high injection throughput of 120 samples/h was achieved. This method was successfully applied to the determination of gentamicin sulfate in pharmaceutical formulations and water samples. Copyright © 2013 John Wiley & Sons, Ltd. PMID:23744581

Iranifam, M; Hasanzadeh, A; Fathinia, M; Khataee, A R; Mousavi, S A

2013-06-01

324

A chemiluminescence method to detect malondialdehyde in plasma and urine.  

PubMed

A highly sensitive and convenient method to detect malondialdehyde (MDA) in specimens of plasma and urine was developed using high-performance liquid chromatography combining chemiluminescence detection. MDA was separated through a reverse-phase C18 column at a flow rate of 0.8mlmin(-1). It was then detected by a chemiluminescence detector. Variables that affected chemiluminescence reaction, including mobile phase, flow rate, chemiluminescence reagent, parameters of the photomultiplier tube, and temperature, were studied. This assay was linear from 0.50 to 50?molL(-1), with limits of detection and quantification of 0.08 and 0.30?molL(-1), respectively. The recoveries were in the range of 92.2 to 98.5%, and the intra- and interday reproducibilities were obtained with relative standard deviations of less than 4% and less than 6%, respectively. MDA in plasma and urine was stable for 12h at 4°C. MDA levels were 2.74, 3.19, and 3.20?molL(-1) for plasma and 1.25, 2.04, and 1.58?molL(-1) for urine, respectively. The proposed method is simple, rapid, and sensitive with low cost. Taken together, this method can be suitable for detecting MDA in tissue and feed specimens. PMID:23973627

Zhang, Guangbin; Tang, Yuhai; Shi, Xiaoyu; Gao, Ruixia; Sun, Yang; Du, Wei; Fu, Qiang

2013-08-21

325

Are the bio- and chemiluminescence states of the firefly oxyluciferin the same as the fluorescence state?  

PubMed

A usual strategy in both experimental and theoretical studies on bio- and chemiluminescence is to analyze the fluorescent properties of the bio- and chemiluminescence reaction product. Recent findings in a coelenteramide and Cypridina oxyluciferin model raise a concern on the validity of this procedure, showing that the light emitters in each of these luminescent processes might differ. Here, the thermal decomposition path of the firefly dioxetanone and the light emission states of the Firefly oxyluciferin responsible for the bio-, chemiluminescence, and fluorescence of the molecule are characterized using ab initio quantum chemistry and hybrid quantum chemistry/molecular mechanics methods to determine if the scenario found in the coelenteramide and Cypridina oxyluciferin study does also apply to the Firefly bioluminescent systems. The results point out to a unique emission state in the bio-, chemiluminescence, and fluorescence phenomena of the Firefly oxyluciferin and, therefore, using fluorescence properties of this system is reasonable. PMID:23057607

Navizet, Isabelle; Roca-Sanjuán, Daniel; Yue, Ling; Liu, Ya-Jun; Ferré, Nicolas; Lindh, Roland

2012-12-17

326

Investigation of singlet oxygen generation in Vit C-Cu2+-LDL system by chemiluminescence method  

NASA Astrophysics Data System (ADS)

In this study, by chemiluminescence method using a Cypridina luciferin analog, 2-methyl-6-(p-methoxyphenyl)-3,7- dihydroimidazo[1,2-a]pyrazin-3-one (MCLA), as a selective and sensitive chemiluminescence probe, singlet oxygen (1O2) formation was observed in the vit C- LDL-Cu2+ reaction system. Another experimental evidence for the generation of 1O2 was the quenching effect of sodium azide (NaN3) on vit C-induced chemiluminescence in the reaction mixture of LDL- Cu2+-MCLA. Analysis based on the experimental results indicated the plausible reaction mechanism is that vit C converts Cu2+ to its reduced state and vit C becomes vit C radical itself, thereby stimulating the formation of peroxyl radicals, and bimolecular reaction of peroxyl radicals results in 1O2 production in the above systems.

Wang, Juan; Xing, Da; Tan, Shici; Tang, Yonghong; He, Yonghong

2002-04-01

327

Applications of Nanomaterials in Electrogenerated Chemiluminescence Biosensors  

PubMed Central

Electrogenerated chemiluminescence (also called electrochemiluminescence and abbreviated ECL) involves the generation of species at electrode surfaces that then undergo electron-transfer reactions to form excited states that emit light. ECL biosensor, combining advantages offered by the selectivity of the biological recognition elements and the sensitivity of ECL technique, is a powerful device for ultrasensitive biomolecule detection and quantification. Nanomaterials are of considerable interest in the biosensor field owing to their unique physical and chemical properties, which have led to novel biosensors that have exhibited high sensitivity and stability. Nanomaterials including nanoparticles and nanotubes, prepared from metals, semiconductor, carbon or polymeric species, have been widely investigated for their ability to enhance the efficiencies of ECL biosensors, such as taking as modification electrode materials, or as carrier of ECL labels and ECL-emitting species. Particularly useful application of nanomaterials in ECL biosensors with emphasis on the years 2004-2008 is reviewed. Remarks on application of nanomaterials in ECL biosensors are also surveyed.

Qi, Honglan; Peng, Yage; Gao, Qiang; Zhang, Chengxiao

2009-01-01

328

Flow-injection chemiluminescence and electrogenerated chemiluminescence determination of escitalopram oxalate in tablet form.  

PubMed

Rapid, simple and highly sensitive flow-injection (FI) chemiluminescence (CL) and flow-injection electrogenerated chemiluminescence (ECL) methods were developed for the determination of escitalopram oxalate (ESC), a selective serotonin reuptake inhibitor used as an antidepressant drug. The CL method was based on the CL reaction of ESC with acidic cerium(IV) and tris(2,2'-bipyridyl)ruthenium(II) (Ru(bipy)(3)(2)+). Various experimental parameters affecting CL intensity were carefully studied and optimised. The method enabled the determination of 0.001-50 µg/mL of ESC in bulk form with a correlation coefficient r = 0.9999. The limit of detection (LOD) was 0.01 ng/mL (S/N = 3). The ECL method was based on the ECL reaction of Ru(bipy)(3)(2)+ with the drug in an acidic medium, permitting the determination of ESC in the range of 0.00001-70 µg/mL with r = 0.9999 and LOD of 1 x 10(-4) ng/mL. The proposed methods were applied to the determination of ESC in commercial tablets. The results were compared statistically with those obtained from a published method using t- and F-tests. PMID:22555899

Alarfaj, Nawal A; Aly, Fatma A; Al-Qahtany, Abeer A

2012-05-04

329

Electrochemistry and electrogenerated chemiluminescence of BODIPY dyes.  

PubMed

BODIPY (boron dipyrromethene) dyes are unique materials with spectroscopic and electrochemical properties comparable to those of aromatic hydrocarbons. Electrochemical studies are useful in understanding the redox properties of these materials and finding structure-stability relations for the radical ions; along with spectroscopy, these studies help researchers design novel compounds with desired properties. This Account represents our attempt at a full description of the electrochemical and electrogenerated chemiluminescence (ECL) properties of the BODIPY dyes. When the dyes are completely substituted with alkyl or other groups, the radical ions of BODIPY dyes are highly stable. But if they include unsubstituted positions, the radical ions can undergo dimerization or other reactions. BODIPY dyes also show unusually large separations, ~1.0 V, between the first and second cyclic voltammetric (CV) waves for both oxidation and reduction half-reactions. Alkyl-substituted BODIPY dyes show good photoluminescence (PL) quantum efficiencies, and radical ion electron transfer annihilation in these molecules produces electrogenerated chemiluminescence (ECL), the intensity of which depends on the structure of the dye. The large separation between waves and the presence of strong ECL signals are both important in the design of stable ECL-based materials. The ECL spectra provide a fast method of monitoring the electrochemical formation of dimers and aggregates from the monomers. BODIPY dyes are particularly good systems for studying stepwise electron transfer in their chemically synthesized oligomers and polymers because of the small separation between the first oxidation and first reduction waves, generally about 2.0-2.4 V, and their relative ease of reduction compared with many other aromatic compounds. The larger separation between consecutive waves for oxidation compared with reduction is noticeable for all BODIPY dimers and trimers. We also observe a more difficult addition or extraction of a third electron compared with the second for the trimers, signaling the importance of electrostatic interactions. In general, BODIPY dyes combine interesting electrochemical and spectroscopic properties that suggest useful analytical applications. PMID:22515199

Nepomnyashchii, Alexander B; Bard, Allen J

2012-04-19

330

Rapid imaging of oxygen free radicals produced in PDT using FCLA chemiluminescence probe  

NASA Astrophysics Data System (ADS)

In this paper, we report a rapid imaging method for oxygen free radicals produced in photodynamic therapy (PDT) in vivo using chemiluminescence probe. The mechanism for PDT involves singlet oxygen (1O2) generated by energy transfer from photosensitizers. 1O2 can react with FCLA, which is a specific chemiluminescence probe for detecting 1O2 and superoxide (O2-). The reaction of FCLA (Fluoresceinyl Cypridina Luminescent Analog) and 1O2 can give emission with peak wavelength at about 532 nm. In the present study, FCLA was chosen as an optical reportere of 1O2 produced from photosensitization reaction of Hematoporphyrin Derivative (HpD) in model solution and in nude mice with transplanted mammary cancer. Photosensitized chemiluminescence from the reaction of FCLA with 1O2 was detected by a highly sensitive Intensified Charge-Coupled Device (ICCD) detector. The chemiluminescence was markedly inhibited by the addition of 10mmol/L sodium azide (NaN3) to the model solution and minor effects were observed at the addition of 10 micrometers ol/L superoxide dismutase (SOD), 20 mmol/L mannitol and 100 (mu) g/mL catalase, respectively, thus indicating that 1O2 generation from photosensitization reaction mainly results in the light emission. Experiments in vivo with tumor-bearing mouse showed a clear chemiluminescence image of tumor.

Xing, Da; Wang, Juan; He, Yonghong

2002-09-01

331

Using a large area CMOS APS for direct chemiluminescence detection in Western blotting electrophoresis  

NASA Astrophysics Data System (ADS)

Western blotting electrophoretic sequencing is an analytical technique widely used in Functional Proteomics to detect, recognize and quantify specific labelled proteins in biological samples. A commonly used label for western blotting is Enhanced ChemiLuminescence (ECL) reagents based on fluorescent light emission of Luminol at 425nm. Film emulsion is the conventional detection medium, but is characterized by non-linear response and limited dynamic range. Several western blotting digital imaging systems have being developed, mainly based on the use of cooled Charge Coupled Devices (CCDs) and single avalanche diodes that address these issues. Even so these systems present key drawbacks, such as a low frame rate and require operation at low temperature. Direct optical detection using Complementary Metal Oxide Semiconductor (CMOS) Active Pixel Sensors (APS)could represent a suitable digital alternative for this application. In this paper the authors demonstrate the viability of direct chemiluminescent light detection in western blotting electrophoresis using a CMOS APS at room temperature. Furthermore, in recent years, improvements in fabrication techniques have made available reliable processes for very large imagers, which can be now scaled up to wafer size, allowing direct contact imaging of full size western blotting samples. We propose using a novel wafer scale APS (12.8 cm×13.2 cm), with an array architecture using two different pixel geometries that can deliver an inherently low noise and high dynamic range image at the same time representing a dramatic improvement with respect to the current western blotting imaging systems.

Esposito, Michela; Newcombe, Jane; Anaxagoras, Thalis; Allinson, Nigel M.; Wells, Kevin

2012-02-01

332

Single-bubble sonochemiluminescence in aqueous luminol solutions.  

PubMed

Sonochemiluminescence (SCL) of luminol due to a single bubble is studied through spectral measurement. No SCL was observed from a stable single bubble that emitted high-intensity sonoluminescence (SL). In contrast, SCL was observed under conditions of an unstable dancing bubble, where a bubble grows and ejects tiny bubbles, making it "dance" by counteraction. Furthermore, SCL was observed from dancing bubbles even when SL was not observed, depending on the dissolved gas content. The instability of bubble collapse is the key parameter governing SCL. PMID:12197706

Hatanaka, Shin-Ichi; Mitome, Hideto; Yasui, Kyuichi; Hayashi, Shigeo

2002-09-01

333

Chemiluminescent Oscillating Demonstrations: The Chemical Buoy, the Lighting Wave, and the Ghostly Cylinder  

ERIC Educational Resources Information Center

|Oscillating reactions have been extensively used in chemical demonstrations. They involve several chemical concepts about kinetics, catalysts, and thermodynamics. The spontaneous cyclic color change of a solution is an attraction in any educational-level course. Chemiluminescent reactions are also among the most fascinating demonstrations and…

Prypsztejn, Hernan E.; Mulford, Douglas R.; Stratton, Doug

2005-01-01

334

Role of background observed in aptasensor with chemiluminescence detection.  

PubMed

One-step chemiluminescent aptasensor was developed using chemically initiated electron exchange luminescence (CIEEL) between high-energy intermediate formed from 1,1'-oxalyldiimidazole chemiluminescence (ODI-CL) reaction and G-quadruplex (ochratoxin A (OTA)-bound aptamer conjugated with TEX615) generated. The sensitivity of chemiluminescent aptasensor, optimized with various variables (e.g., property of microfibers fabricated with 3,4,9,10-perylenetetracarboxylic dimide, determination of fluorescent dye labeled with aptamer, physical properties of buffer solution), was dependent on the background (concentration of high-energy intermediate) generated in ODI-CL reaction. The limit of detection (LOD=background+3×standard deviation, 0.5nM) of ODI-CL aptasensor with lower background was lower than that (3.7nM) with 20 times higher background. Also, the ratio of signal to background (S/B) of ODI-CL aptasensor with low background was about 5-fold higher than that with high background. The sensitivities of ODI-CL aptasensors, with low as well as high background, capable of accurately and precisely quantifying OTA within 10min, were better than those of fluorescent aptasensors and as good as those of highly sensitive but time-consuming competitive enzyme-linked immune-sorbent assays (ELISAs) using expensive antibody produced with the sacrifice of small animals. PMID:24148468

Park, Lucienne; Kim, Julie; Lee, Ji Hoon

2013-08-02

335

[Modification of the chemiluminescent analysis method for evaluation of the whole blood phagocyte activity in sensibilized animals].  

PubMed

A modified method of chemiluminescence analysis was developed in experiments with animals. Whole blood of guinea pigs was made use of as the biological sample. BaSO4 and another new substance, i.e. aerosol, were used in the phagocytes' stimulation of whole blood. Optimal concentrations of reaction components, the incubation period of whole blood samples and of specific allergens and the duration of chemiluminescence registration were defined. The discussed modified method of chemiluminescence analysis can be used to monitor the specific treatment schemes by immunotherapy and to choose adequate schemes for the mentioned therapy. PMID:15137205

Mitereva, D E; Agafonov, V E

2004-03-01

336

Chemiluminescence microanalysis of substrates and enzymes.  

PubMed

Extracts from bioluminescent organisms are increasingly used for analysis of small amounts of substrates and enzymes. The light emission is in some organisms related to the conversion of substrates and cofactors of central metabolic importance. Extracts from such organisms are particularly valuable for analytical applications. This is quite obvious in the firefly where the energy, required for light production, is derived from ATP and in a couple of strains of luminescent bacteria where reduced pyridine nucleotides through reduction of flavine mononucleotide is utilized in the light reaction. It deserved to be noted that many biochemical reactions can be coupled more or less directly to the conversion of ATP, NAD(H), NADP(H) and FMN(H), thus providing the basis for a great variety of analyses. Special kinds of bioluminescent reactions are also of considerable interest, as for instance the relationship between "active sulphate" and PAP, which participate in the formation of light in the sea pansy (Renilla reniformis). High sensitivities are often reached in chemiluminescence analysis making the technique suitable for samples composed of a small number of cells. How bioluminescence has been employed in these kinds of microanalyses is examplified in studies of nucleotides, metabolites and enzymes with low activities. PMID:14428

Brolin, S E; Wettermark, G; Hammar, H

1977-02-01

337

CHEMILUMINESCENCE ASSAY FOR BACTERIUM HELICOBACTER PYLORI DNA  

Microsoft Academic Search

A chemiluminescence dot blot hybridization assay for Helicobacter pylori (H. pylori) DNA was established. A 20-mer oligo-nucleotide probe labeled directly alkaline phosphatase (AP) and highly sensitive chemiluminescence substrate for AP were used to detect H. pylori DNA. Preliminary H. pylori DNA testing was carried out on gastric biopsy specimens of 24 cases from patients. The results of detection were compared

Yang Chen; Zuhong Lu; Guoping Xia

2001-01-01

338

Bio and chemiluminescence imaging in analytical chemistry  

Microsoft Academic Search

Bio- and chemiluminescence imaging techniques combine the high sensitivity of bio- and chemiluminescence detection with the ability of current light imaging devices to localize and quantify light emission down to the single-photon level. These techniques have been successfully exploited for the development of sensitive analytical methods relying on the evaluation of the spatial distribution of the light emitted from a

Aldo Roda; Massimo Guardigli; Patrizia Pasini; Mara Mirasoli; Elisa Michelini; Monica Musiani

2005-01-01

339

Inhibitory effect of stobadine on FMLP-induced chemiluminescence in human whole blood and isolated polymorphonuclear leukocytes.  

PubMed

The chemiluminescence (CL) technique with luminol and isoluminol was used to characterize the effect of stobadine on reactive oxygen metabolites (ROM) generation in human whole blood and in isolated polymorphonuclear leukocytes (PMNL) stimulated with N-formyl-methionyl-leucyl-phenyl-alanine (FMLP). In whole blood and in isolated PMNL, stobadine in the concentrations of 1, 10 and 100 micromol/L significantly inhibited the CL signal after FMLP, which activated predominantly extracellular generation of ROM. The same concentrations of stobadine were effective on CL in a cell-free system. On the other hand, myeloperoxidase (MPO) liberation was decreased by stobadine only in the concentration of 100 micromol/L. The results showed stobadine to act as a potent inhibitor/scavenger of extracellularly produced ROM in human PMNL and indicated interference of stobadine with ROM as well as with signalling events resulting in NADPH-oxidase activation and MPO liberation. PMID:16871535

Drábiková, Katarína; Jancinová, Viera; Nosál, Radomír; Pecivová, Jana; Macicková, Tatiana; Turcáni, Peter

340

An evaluation of luminol formulations and their effect on DNA profiling.  

PubMed

Luminol is a presumptive test reagent used for the location of latent bloodstains. Various formulations are used by different forensic practitioners and commercial products are also widely available. There is little concurrence between authors with regards to the sensitivity limits of luminol which can vary significantly depending upon the substrate. We evaluated the sensitivity and stability of five different luminol formulations on a range of blood dilutions. All formulations showed an overall decrease in performance over 24 h though the effect was more gradual on a non-porous surface compared to porous. We found that BlueStar® Magnum showed the greatest sensitivity compared to other formulations and detected 50 ?l of 1/100,000 blood dilutions on both porous and non-porous surfaces. Two formulations of luminol were selected based on the result of the sensitivity and stability study and were assessed for their impact on the DNA profiling process. There was a statistically significant improvement in DNA profile peak area from luminol-treated samples when compared to control samples of neat blood stains. However, at the weaker blood dilution of 1/1,000, the difference between control and luminol-treated samples was dependent on the substrate type with porous (fabric) samples showing a significant difference and non-porous (tile) swabbed samples requiring further work to conclusively ascertain the effect. PMID:23263409

Patel, Gnyaneshwari; Hopwood, Andy

2012-12-21

341

Microfluidic enzyme immunosensors with immobilised protein A and G using chemiluminescence detection.  

PubMed

Affinity proteins were covalently immobilised on silicon microchips with overall dimensions of 13.1 x 3.2 mm, comprising 42 porous flow channels of 235 microm depth and 25 microm width, and used to develop microfluidic immunosensors based on horseradish peroxidase (HRP), catalysing the chemiluminescent oxidation of luminol/p-iodophenol (PIP). Different hydrophilic polymers with long flexible chains (polyethylenimine (PEI), dextran (DEX), polyvinyl alcohol, aminodextran) and 3-aminopropyltriethoxysilane (APTS) were employed for modification of the silica surfaces followed by attachment of protein A or G. The resulting immunosensors were compared in an affinity capture assay format, where the competition between the labelled antigen and the analyte for antibody-binding sites took place in the bulk of the solution. The formed immunocomplexes were then trapped by the microchip affinity capture support and the amount of bound tracer was monitored by injection of luminol, PIP and H2O2. All immunosensors were capable of detecting atrazine at the sub-microg l(-1) level. The most sensitive assays were obtained with PEI and DEX polymer modified supports and immobilised protein G, with limits of detection of 0.006 and 0.010 microg l(-1), and IC50 values of 0.096 and 0.130 microg l(-1), respectively. The protein G based immunosensors were regenerated with 0.4 M glycine-HCl buffer pH 2.2, with no loss of activity observed for a storage and operating period of over 8 months. To estimate the applicability of the immunosensors to the analysis of real samples, PEI and DEX based protein G microchips were used to detect atrazine in surface water and fruit juice, spiked with known amounts of the atrazine, giving recovery values of 87-102 and 88-124% at atrazine fortification levels of 0.5-3 and 80-240 microg l(-1), respectively. PMID:14558995

Yakovleva, Julia; Davidsson, Richard; Bengtsson, Martin; Laurell, Thomas; Emnéus, Jenny

2003-10-30

342

Interferometric Study of the Chemiluminescent Excitation of Sodium by Active Nitrogen.  

National Technical Information Service (NTIS)

Lines shapes of the Na(D2) emission (5890A) from the chemiluminescent reaction of active nitrogen with sodium have been measured in a flow system using a piezoelectric scanning Fabry--Perot interferometer. The sodium Doppler temperature is about 1500K. Ex...

R. G. Gann F. Kaufman M. A. Biondi

1972-01-01

343

Optical sensor for the determination of glucose based on KIO4 chemiluminescence detection.  

PubMed

A method to determine glucose using an optical sensor prepared by entrapping glucose oxidase into silica sol-gel column has been developed. The silica sol-gel film was coated on alumina substrate. The optical sensor is based on the chemiluminescence intensity from the reaction of periodate and hydrogen peroxide in K2CO3 medium. The effect of the ratio of water and alcohol for the preparation of TEOS sol on chemiluminescence intensity was investigated. The effects of pH of enzyme reactor, concentrations of potassium periodate and SDS, and flow rate on the chemiluminescence intensity were studied to find the optimum experimental conditions to determine glucose. The chemiluminescence intensity increased linearly with increasing glucose concentration from 5.0 x 10(-4) M to 1.0 x 10(-7) M and the detection limit was 4.0 x 10(-8) M. Interference effects from some metal ions on chemiluminescence intensity were also investigated. PMID:15617266

Li, Ming; Lee, Sang Hak; Bae, Zun Ung; Lee, Kwang Pill; Park, Yu Chul; Lee, Mu Sang

2004-09-01

344

Synthesis of a novel chemiluminescent reagent for the determination of hydrogen peroxide in snow waters.  

PubMed

7-(4,6-Dichloro-1,3,5-triazinylamino)-4-methylcoumarin (DTMC) was synthesized as a completely new chemiluminescent reagent, and with it a novel chemiluminescence method was developed for the determination of hydrogen peroxide in the absence of any added catalyst or co-oxidant. The chemiluminescence intensity of the DTMC-H(2)O(2) system could be enhanced by the addition of cation surfactants. The chemiluminescence intensity was directly proportional to the concentration of H(2)O(2) in the range 1.0 x 10(-7)-4.0x10(-4) mol l(-1), and the detection limit was 4.0 x 10(-8) mol l(-1). The relative S.D. was 4.9% for 1.0 x 10(-6) mol l(-1) of H(2)O(2) (n=10). The selectivity of this method was high, and most of the transition metal ions have no effect on the determination. The proposed method has been applied to the determination of trace amounts of hydrogen peroxide in snow water. A possible mechanism of the chemiluminescence reaction is also discussed. PMID:18968188

Ma, Q; Ma, H; Wang, Z; Su, M; Xiao, H; Liang, S

2001-01-26

345

Storable, thermally activated, near-infrared chemiluminescent dyes and dye-stained microparticles for optical imaging  

PubMed Central

Optical molecular imaging employs relatively harmless, low-energy light and technically straightforward instrumentation. Self-illuminating, chemiluminescent systems are especially attractive since they have inherently high signal contrast due to the lack of background emission. Currently, chemiluminescence imaging involves short-lived molecular species that are not stored but instead generated in situ, and they typically emit visible light, which does not penetrate far through heterogeneous biological media. Here, we describe a new paradigm for optical molecular imaging using squaraine rotaxane endoperoxides (SREPs), interlocked fluorescent and chemiluminescent dye molecules that have a squaraine chromophore encapsulated inside a macrocycle endoperoxide. SREPs can be stored indefinitely at temperatures below ?20 °C, but upon warming to body temperature they undergo a unimolecular chemical reaction and emit near infrared light that can pass through a living mouse. Dye-stained microparticles are easily prepared for in vivo near-infrared optical imaging using commercial imaging stations.

Baumes, Jeffrey M.; Gassensmith, Jeremiah J.; Giblin, Jay; Lee, Jung-Jae; White, Alexander G.; Culligan, William J.; Leevy, W. Matthew; Kuno, Masaru; Smith, Bradley D.

2011-01-01

346

Halocarbon Interferences in Chemiluminescent Measurements of NOx.  

National Technical Information Service (NTIS)

Anomalous NOx responses were observed when halocarbons were irradiated in the presence of oxides of nitrogen. Interferences to chemiluminescent NOx monitor using heated carbon converter were studied for phosgene, tri-chloroacetyl chloride, chloroform, chl...

S. B. Joshi J. J. Bufalini

1978-01-01

347

HALOCARBON INTERFERENCES IN CHEMILUMINESCENT MEASUREMENTS OF NOX  

EPA Science Inventory

Anomalous NOx responses were observed when halocarbons were irradiated in the presence of oxides of nitrogen. Interferences to chemiluminescent NOx monitor using heated carbon converter were studied for phosgene, tri-chloroacetyl chloride, chloroform, chlorine, and hydrochloric a...

348

Chemiluminescence Immunoassay (CLIA) for Candida Antigen.  

National Technical Information Service (NTIS)

The project demonstrated that the combination of chemiluminescent (CL) acridinium esters and monoclonal antibodies to Candida cell-wall mannan is capable of generating a simple non-isotopic immunoassay for Candida mannan in serum. The sensitivity of the i...

A. Patel

1987-01-01

349

Long-lived chemiluminescence in cigarette smoke  

Microsoft Academic Search

Cigarette smoke contains high concentrations of unstable molecules that react with oxygen to produce chemiluminescence. The chemiluminescent activity concentrated in the aerosol phase that can be absorbed on glass-fiber filters and extracted into organic solvents. Cigarette smoke in N,N-dimethylformamide produces a long-lasting luminescence visible to the dark-adapted eye. We have demonstrated the oxygen dependence and have measured the kinetics, activation

H. H. Seliger; W. H. Biggley; J. P. Hamman

1974-01-01

350

Long-Lived Chemiluminescence in Cigarette Smoke  

Microsoft Academic Search

Cigarette smoke contains high concentrations of unstable molecules that react with oxygen to produce chemiluminescence. The chemiluminescent activity is concentrated in the aerosol phase that can be absorbed on glass-fiber filters and extracted into organic solvents. Cigarette smoke in N,N-dimethylformamide produces a long-lasting luminescence visible to the dark-adapted eye. We have demonstrated the oxygen dependence and have measured the kinetics,

H. H. Seliger; W. H. Biggley; J. P. Hamman

1974-01-01

351

Multicolor Directional Surface Plasmon-Coupled Chemiluminescence  

PubMed Central

In reports over the past several years, we have demonstrated the efficient collection of optically excited fluorophore emission by its coupling to surface plasmons on thin metallic films, where the coupled luminescence was highly directional and polarized. This phenomenon is referred to as surface plasmon-coupled emission (SPCE). In this current study, we have extended this technique to include chemiluminescing species and subsequentially now report the observation of surface plasmon-coupled chemiluminescence (SPCC), where the luminescence from chemically induced electronic excited states couples to surface plasmons in thin continuous metal films. The SPCC is highly directional and predominantly p-polarized, strongly suggesting that the emission is from surface plasmons instead of the luminophores themselves. This indicates that surface plasmons can be directly excited from chemically induced electronic excited states and excludes the possibility that the plasmons are created by incident excitation light. This phenomenon has been observed for a variety of chemiluminescent species in the visible spectrum, ranging from blue to red, and also on a variety of metals, namely, aluminum, silver, and gold. Our findings suggest new chemiluminescence sensing strategies on the basis of localized, directional, and polarized chemiluminescence detection, especially given the wealth of assays that currently employ chemiluminescence-based detection.

Chowdhury, Mustafa H.; Malyn, Stuart N.; Aslan, Kadir; Lakowicz, Joseph R.; Geddes, Chris D.

2009-01-01

352

Sorption of platinum on immobilized microorganisms for its on-line preconcentration and chemiluminescent determination in water samples.  

PubMed

Fungi of the type Aspergillus sp. were immobilized on a cellulosic resin and used as a biosorbent for the on-line preconcentration and separation of Pt(IV) ions prior to their chemiluminescent determination via flow injection analysis. Biosorption and elution conditions were optimized, and the results compared to biosorbents based on the use of Chlorella vulgaris algae and Saccharomyces cerevisiae yeast in terms of preconcentration and selective retention of Pt(IV). The immobilized fungi presented here have a high potential for use in platinum biosorption. The procedure exhibits the currently lowest limit of detection (0.02 ng mL(-1) of Pt) and very high selectivity. The procedure was applied to the determination of Pt(IV) in river water, road run-off, and wastewater samples.FigureSchematic diagram of flow injection manifold for on-line preconcentration/separation of Pt(IV) on immobilized fungi followed by its luminol-based chemiluminescent determination. The CL-FIA manifold was applied to the determination of platinum in river water, road run-off, and wastewater samples. PMID:22347728

Malejko, Julita; Szyga?owicz, Marzena; Godlewska-?y?kiewicz, Beata; Koj?o, Anatol

2011-11-20

353

Toxicity of pyrimidine derivatives under oxidative stress conditions: chemiluminescence-based assays in systems containing erythrocytes, mitochondria or blood plasma.  

PubMed

Participation of mono-, di-, and tricyclic pyrimidine derivatives in free radical processes was investigated with the use of luminol-enhanced chemiluminescence in measurements performed in vitro in systems containing erythrocytes, erythrocyte lysate, erythrocyte membranes, mitochondria, mitoplasts or blood plasma. The free radical processes were induced in the investigated systems by tert -butyl hydroperoxide ( t -BuOOH) or 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). Amongst the examined compounds, only derivatives containing the thiol substituent were found to modify the course of chemiluminescence. Effects of both amplification and inhibition of light emission were observed to depend on the structure of a derivative and on the type of a biological (experimental) system. The light emission-amplifying activity was found to be characteristic of the system containing erythrocytes. The results of the investigations point out that the pyrimidine thio-derivatives prolonged the oxidative stress through some interactions mainly with (oxy)hemoglobin, which was related with specific toxicity against erythrocytes. Model investigations in the proposed experimental systems can serve as a useful tool at early stages of the drug discovery process when compounds involved in the interactions connected with the oxidative stress are being selected. On the other hand, this method allows to study mechanisms of the toxic action of xenobiotics on cells (erythrocytes) and organelles (mitochondria), strongly implicated in the free radical generation, and to examine the role of the extracellular liquid (plasma). PMID:17556799

Sajewicz, Waldemar

354

A compactly integrated flow cell with a chemiluminescent FIA system for determining lactate concentration in serum.  

PubMed

We have fabricated an integrated flow cell as a total microanalysis system (microTAS). This flow cell (size, 15 x 20 mm; total inner volume, 12.2 microL) was designed for a rational analyzing system of lactate determination for serum. This cell was made by micromachining techniques and consisted of two hollows of a lactate oxidase (LOD) reactor and a mixing cell, a spiral groove, and three penetrated holes. To form the reactor and capillary, these patterns, etched on a silicon wafer, were attached to a glass plate by the anodic bonding method. A photodiode was put under part of the spiral capillary. The compactly accumulated devices were integrated into a flow injection analysis (FIA) system. In the flow cell, lactate was catalyzed to pyruvate and hydrogen peroxide at the LOD reactor; subsequently, hydrogen peroxide reacted with the luminol-ferricyanic reagent at the mixing cell. The resulting chemiluminescent light was detected by the photodiode. Using the miniaturized flow cell, the sample volume for one measurement was greatly reduced to 0.2 microL. The response to lactate was obtained within 30 s and was linear between 0.5 and 5.0 mM (4.5 and 45 mg/dL) lactate with excellent correlative variances of 3.2% (average of three measurements at 5.0 mM). For practical application, the lactate concentration in control human serum was determined using this system. The results showed a good correlation coefficient (r = 0.979) with the results obtained by the spectrophotometric reference method. No difference in sera (normal or pathological) was found. Consequently, this integrated flow cell shows potential as a clinical device for lactate determination in serum. In this article, the effect of the design on the chemiluminescent FIA system is also described. PMID:11199992

Nakamura, H; Murakami, Y; Yokoyama, K; Tamiya, E; Karube, I; Suda, M; Uchiyama, S

2001-01-15

355

Selective Detection of Neurotransmitters by Fluorescence and Chemiluminescence Imaging  

SciTech Connect

In recent years, luminescence imaging has been widely employed in neurochemical analysis. It has a number of advantages for the study of neuronal and other biological cells: (1) a particular molecular species or cellular constituent can be selectively visualized in the presence of a large excess of other species in a heterogeneous environment; (2) low concentration detection limits can be achieved because of the inherent sensitivity associated with fluorescence and chemiluminescence; (3) low excitation intensities can be used so that long-term observation can be realized while the viability of the specimen is preserved; and (4) excellent spatial resolution can be obtained with the light microscope so subcellular compartments can be identified. With good sensitivity, temporal and spatial resolution, the flux of ions and molecules and the distribution and dynamics of intracellular species can be measured in real time with specific luminescence probes, substrates, or with native fluorescence. A noninvasive detection scheme based on glutamate dehydrogenase (GDH) enzymatic assay combined with microscopy was developed to measure the glutamate release in cultured cells from the central nervous system (CNS). The enzyme reaction is very specific and sensitive. The detection limit with CCD imaging is down to {micro}M levels of glutamate with reasonable response time. They also found that chemiluminescence associated with the ATP-dependent reaction between luciferase and luciferin can be used to image ATP at levels down to 10 nM in the millisecond time scale. Similar imaging experiments should be feasible in a broad spectrum of biological systems.

Ziqiang Wang; Edward S. Yeung

2001-08-06

356

Gold nanorods-enhanced rhodamine B-permanganate chemiluminescence and its analytical application  

NASA Astrophysics Data System (ADS)

A novel enhanced chemiluminescence system was developed by applying gold nanorods (Au NRs) as catalysts in rhodamine B-permanganate reaction. Au NRs with three different aspect ratios were synthesized by seed mediated growth method and characterized by UV-Vis spectra and transmission electron microscopy. It was demonstrated that Au NRs have much higher catalytic effect than spherical nanoparticles on rhodamine B-permanganate chemiluminescence reaction. Among various sizes of Au NRs, those with average aspect ratio of 3.0 were found to have the most remarkable catalytic activity. As an analytical application of the new chemiluminescence system, albumin as a model protein was quantified based on its interaction with NRs. Albumin binds to Au NRs active surfaces and inhibits their catalytic action and therefore decreases the intensity of chemiluminescence. This diminution effect is linearly related to the concentration of the human and bovine serum albumin over the ranges of 0.45-90 and 0.75-123 nmol L-1, respectively with the corresponding limits of detection of 0.18 and 0.30 nmol L-1. The method was successfully applied to the determination of albumin in human and bovine serum samples.

Hassanzadeh, Javad; Amjadi, Mohammad; Manzoori, Jamshid L.; Sorouraddin, Mohammad Hossein

2013-04-01

357

Spatial resolution of a chemiluminescence sensor for local heat-release rate and equivalence ratio measurements in a model gas turbine combustor  

NASA Astrophysics Data System (ADS)

The spatial resolution of a Chemiluminescence Sensor, based on focused Cassegrain optics, to detect the location of the reaction zone and heat-release rate in a model gas turbine combustor is reported. The sensor measures simultaneously the chemiluminescent intensities from OH* and CH* excited radicals in flames in order to obtain information on the local flame characteristics. The spatial resolution was evaluated by a combined theoretical and experimental study in laminar and turbulent flames and was supported by detailed chemistry calculations, including the chemiluminescent species, of unstrained one-dimensional flames. The experimental study involved simultaneous measurements of chemiluminescence with the sensor and laser-based reaction rate imaging, using the product of OH and CH2O radicals obtained from planar laser-induced fluorescence (PLIF), and OH PLIF for the location of the reaction zone. The study quantified the influence of flame shape and dimensions and the direction of traverse of the focal region of the sensor through the flames on the spatial resolution, thereby identifying the limitations and optimising the applicability of the sensor. The sensor was used to obtain local time-dependent measurements of heat-release and equivalence ratio of a reacting mixture, based on the chemiluminescent intensity ratio of OH*/CH*, in a swirl-stabilised model gas turbine combustor and quantified the degree of air-fuel premixedness, probability of reaction and power spectra of pressure and chemiluminescent intensity fluctuations in two unsteady flames.

Hardalupas, Y.; Panoutsos, C. S.; Taylor, A. M. K. P.

2010-10-01

358

Chemiluminescence response induced by mesenteric ischaemia/reperfusion: effect of antioxidative compounds ex vivo  

PubMed Central

Ischaemia and reperfusion (I/R) play an important role in human pathophysiology as they occur in many clinical conditions and are associated with high morbidity and mortality. Interruption of blood supply rapidly damages metabolically active tissues. Restoration of blood flow after a period of ischaemia may further worsen cell injury due to an increased formation of free radicals. The aim of our work was to assess macroscopically the extent of intestinal pathological changes caused by mesenteric I/R, and to study free radical production by luminol enhanced chemiluminescence (CL) of ileal samples. In further experiments, the antioxidative activity of the drugs tested was evaluated spectrophotometrically by the use of the DPPH radical. We studied the potential protective ex vivo effect of the plant origin compound arbutin as well as of the pyridoindole stobadine and its derivative SMe1EC2. I/R induced pronounced haemorrhagic intestinal injury accompanied by increase of myeloperoxidase (MPO) and N-acetyl-?-D-glucosaminidase (NAGA) activity. Compared to sham operated (control) rats, there was only a slight increase of CL response after I/R, probably in association with neutrophil increase, indicated by enhanced MPO activity. All compounds significantly reduced the peak values of CL responses of the ileal samples ex vivo, thus reducing the I/R induced increase of free radical production. The antioxidants studied showed a similar inhibitory effect on the CL response influenced by mesenteric I/R. If proved in vivo, these compounds would represent potentially useful therapeutic antioxidants.

Nosal'ova, Viera; Sotnikova, Ruzena; Drabikova, Katarina; Fialova, Silvia; Kostalova, Daniela; Banasova, Silvia; Navarova, Jana

2010-01-01

359

Low-level chemiluminescent analysis of nondiluted human blood reveals its dynamic system properties  

NASA Astrophysics Data System (ADS)

Lucigenin- and luminol-dependent chemiluminescence [(LC- CL) and (LM-CL)] in nondiluted human blood was studied. LM-CL was low in fresh blood and disappeared after its storage for 3 h, though the respiratory burst (RB) stimulated in blood was followed by high intensity and long- lasting LM-CL. LC-CL was high in fresh blood and was steadily increasing with blood storage. Blood dilution with saline resulted in LC-CL attenuation and LM-CL elevation. LC-CL did not depend on air supply to blood, while LM-CL elevation during RB needed constant blood aeration. The results suggest that besides a well-known mechanism of reactive oxygen species production by neutrophils during RB, another process of electron excited state generation reflected by LC-CL operates in blood. It needs blood integrity for its manifestation and uses oxygen supplied by erythrocytes. Dynamic system properties of blood were revealed also in experiments with blood transfer from one sample to another in the course of RB. Highly nonlinear changes of CL intensity both in a `donor' and in a `recipient' sample resulted in strong differences in CL levels in two samples, one of which was prepared by blood subtraction, and another by blood addition. We suggest that CL data from measurements on nondiluted blood may be informative of integrative properties of blood tissue in addition to its being a measure of some sort of oxidative metabolism in it.

Voeikov, Vladimir L.; Novikov, Cyril N.; Vilenskaya, Natalia D.

1999-01-01

360

Flow-injection post-chemiluminescence method for the determination of palmatine.  

PubMed

A post-chemiluminescence (PCL) phenomenon was observed when palmatine was injected in a mixture of N-bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF) after the chemiluminescence (CL) reaction of NBS-alkaline DCF had finished. Based on the PCL reaction, a rapid and sensitive method for the determination of palmatine was established. Under optimum conditions, the CL intensity was linear, with the concentration of palmatine in the range of 5.0 × 10(-9) to 1.0 × 10(-6) M. The detection limit was 6.0 × 10(-10) M for palmatine. The relative standard deviation for 11 parallel measurements of 1.0 × 10(-7) M palmatine was 1.5%. This method was applied to the determination of palmatine in pharmaceutical samples and biological fluids, with satisfactory results. The possible reaction mechanism is discussed briefly. PMID:23816126

Han, Suqin; Wei, Bei; Hao, Fang

2013-07-01

361

Numerical evaluation of equivalence ratio measurement using OH ? and CH ? chemiluminescence in premixed and non-premixed methane–air flames  

Microsoft Academic Search

This work presents results from detailed chemical kinetics calculations of electronically excited OH (A2?, denoted as OH?) and CH (A2?, denoted as CH?) chemiluminescent species in laminar premixed and non-premixed counterflow methane–air flames, at atmospheric pressure. Eight different detailed chemistry mechanisms, with added elementary reactions that account for the formation and destruction of the chemiluminescent species OH? and CH?, are

C. S. Panoutsos; Y. Hardalupas; A. M. K. P. Taylor

2009-01-01

362

Numerical evaluation of equivalence ratio measurement using OH{sup *} and CH{sup *} chemiluminescence in premixed and non-premixed methane-air flames  

Microsoft Academic Search

This work presents results from detailed chemical kinetics calculations of electronically excited OH (A², denoted as OH{sup *}) and CH (A², denoted as CH{sup *}) chemiluminescent species in laminar premixed and non-premixed counterflow methane-air flames, at atmospheric pressure. Eight different detailed chemistry mechanisms, with added elementary reactions that account for the formation and destruction of the chemiluminescent species OH{sup *}

C. S. Panoutsos; Y. Hardalupas; A. M. K. P. Taylor

2009-01-01

363

Spatial resolution of a chemiluminescence sensor for local heat-release rate and equivalence ratio measurements in a model gas turbine combustor  

Microsoft Academic Search

The spatial resolution of a Chemiluminescence Sensor, based on focused Cassegrain optics, to detect the location of the reaction zone and heat-release rate in a model gas turbine\\u000a combustor is reported. The sensor measures simultaneously the chemiluminescent intensities from OH* and CH* excited radicals\\u000a in flames in order to obtain information on the local flame characteristics. The spatial resolution was

Y. HardalupasC; C. S. Panoutsos; A. M. K. P. Taylor

2010-01-01

364

Determination of phenol at ng l -1 level by flow-injection chemiluminescence combined with on-line solid-phase extraction  

NASA Astrophysics Data System (ADS)

In this paper, a simple flow-injection chemiluminescence (CL) system combined with on-line solid-phase extraction is presented to determine phenol. This method is based on the enhancement effect of phenol on the luminol-K 3Fe(CN) 6 CL system. The solid-phase extraction promised the high sensitivity and improved selectivity of CL detection. With the calibration range from 4.7 ng l -1 to 470 ng l -1 phenol concentration, the proposed method was applied to analyzing phenol in water samples and the obtained results were validated by the standard method. The detection limit was determined as 0.66 ng l -1. The relative standard deviation was 1.5% for determining 4.7 ng l -1 phenol standard ( n = 7).

Qi, Huili; Lv, Jiagen; Li, Baoxin

2007-04-01

365

Assessment of nitric oxide signals by triiodide chemiluminescence  

PubMed Central

Nitric oxide (NO) bioactivity is mainly conveyed through reactions with iron and thiols, furnishing iron nitrosyls and S-nitrosothiols with wide-ranging stabilities and reactivities. Triiodide chemiluminescence methodology has been popularized as uniquely capable of quantifying these species together with NO byproducts, such as nitrite and nitrosamines. Studies with triiodide, however, have challenged basic ideas of NO biochemistry. The assay, which involves addition of multiple reagents whose chemistry is not fully understood, thus requires extensive validation: Few protein standards have in fact been characterized; NO mass balance in biological mixtures has not been verified; and recovery of species that span the range of NO-group reactivities has not been assessed. Here we report on the performance of the triiodide assay vs. photolysis chemiluminescence in side-by-side assays of multiple nitrosylated standards of varied reactivities and in assays of endogenous Fe- and S-nitrosylated hemoglobin. Although the photolysis method consistently gives quantitative recoveries, the yields by triiodide are variable and generally low (approaching zero with some standards and endogenous samples). Moreover, in triiodide, added chemical reagents, changes in sample pH, and altered ionic composition result in decreased recoveries and misidentification of NO species. We further show that triiodide, rather than directly and exclusively producing NO, also produces the highly potent nitrosating agent, nitrosyliodide. Overall, we find that the triiodide assay is strongly influenced by sample composition and reactivity and does not reliably identify, quantify, or differentiate NO species in complex biological mixtures.

Hausladen, Alfred; Rafikov, Ruslan; Angelo, Michael; Singel, David J.; Nudler, Evgeny; Stamler, Jonathan S.

2007-01-01

366

Highly sensitive flow-injection chemiluminescence determination of pyrogallol compounds  

NASA Astrophysics Data System (ADS)

A highly sensitive flow-injection chemiluminescent method for the direct determination of pyrogallol compounds has been developed. Proposed method is based on the enhanced effect of pyrogallol compounds on the chemiluminescence signals of KMnO 4-H 2O 2 system in slightly alkaline medium. Three important pyrogallol compounds, pyrogallic acid, gallic acid and tannic acid, have been detected by this method, and the possible mechanism of the CL reaction is also discussed. The proposed method is simple, convenient, rapid (60 samples h -1), and sensitive, has a linear range of 8 × 10 -10 mol L -1 to 1 × 10 -5 mol L -1, for pyrogallic acid, with a detection limit of 6 × 10 -11 mol L -1, 4 × 10 -8 mol L -1 to 5 × 10 -3 mol L -1 for gallic acid with a detection limit of 9 × 10 -10 mol L -1, and 8 × 10 -8 mol L -1 to 5 × 10 -2 mol L -1 for tannic acid, with a detection limit of 2 × 10 -9 mol L -1, respectively. The relative standard deviation (RSD, n = 15) was 0.8, 1.1 and 1.3% for 5 × 10 -6 mol L -1 pyrogallic acid, gallic acid and tannic acid, respectively. The proposed method was successfully applied to the determination of pyrogallol compounds in tea and coffee samples.

Kanwal, Shamsa; Fu, Xiaohong; Su, Xingguang

2009-12-01

367

A novel green analytical procedure for monitoring of azoxystrobin in water samples by a flow injection chemiluminescence method with off-line ultrasonic treatment.  

PubMed

A simple and green flow injection chemiluminescence (FI-CL) method for determination of the fungicide azoxystrobin was described for the first time. CL signal was generated when azoxystrobin was injected into a mixed stream of luminol and KMnO4 . The CL signal of azoxystrobin could be greatly improved when an off-line ultrasonic treatment was adopted. Meanwhile, the signal intensity increases with the analyte concentration proportionally. Several variables, such as the ultrasonic parameters, flow rate of reagents, concentrations of sodium hydroxide solution and CL reagents (potassium permanganate, luminol) were investigated, and the optimal CL conditions were obtained. Under optimal conditions, the linear range of 1-100?ng/mL for azoxystrobin was obtained and the detection limit (3?) was determined as 0.13?ng/mL. The relative standard deviation was 1.5% for 10 consecutive measurements of 20?ng/mL azoxystrobin. The method has been applied to the determination of azoxystrobin residues in water samples. Copyright © 2012 John Wiley & Sons, Ltd. PMID:23027659

Yang, Xin-An; Zhang, Wang-Bing

2012-10-02

368

Chemiluminescence spectra to thioformaldehyde and selenoformaldehyde  

SciTech Connect

Moderate-resolution chemiluminescence spectra of CD{sub 2}S, CH{sub 2}S, and CH{sub 2}Se were obtained during the reaction of F{sub 2} with methylated sulfur and selenium compounds at about 0.5 Torr of total pressure. Phosphorescence corresponding to the {tilde a}{sup 3}A{sub 2} {yields} {tilde X}{sup 1}A{sub 1} transition dominated all the spectra, and a trace of fluorescence, {tilde A}{sup 1}A{sub 2} {yields} {tilde X}{sup 1}A{sub 1}, appeared only in the spectrum of CH{sub 2}Se. The v{sub 4} and v{sub 6} frequencies of the {tilde a} state of CD{sub 2}S are reported for the first time as 219.4 {plus minus} 5.0 and 572.6 {plus minus} 5.0 cm{sup {minus}1}, respectively. From the unusually shaped 4{sub 1}{sup 1} band of CH{sub 2}S ({tilde a} {yields} {tilde X}) an improved value of the v{prime}{sub 4} frequency was found to be 308 {plus minus} 30 cm{sup {minus}1}. The CH{sub 2}Se yielded an improved measurement of the v{double prime}{sub 3} frequency, 860 {plus minus} 10 cm{sup {minus}1}, and new estimates of three other ground-state frequencies: v{double prime} = 1,460 {plus minus} 30, v{double prime}{sub 4} = 906 {plus minus} 10, and v{double prime} = 914 {plus minus} 10, and v{double prime}{sub 6} = 914 {plus minus} 20 cm{sup {minus}1}. A spectrum of CH{sub 2}Te was sought in the reactions of CH{sub 3}TeCH{sub 3} and CH{sub 3}Te{sub 2}CH{sub 3} with F{sub 2} but was not found; instead emission from TeF (A{sup 2}{pi}{sub i} {yields} X{sup 2}{pi}{sub 1}) was observed.

Glinski, R.J.; Taylor, C.D.; Martin, H.R. (Tennessee Technological Univ., Cookeville (United States))

1991-08-08

369

Chemiluminescence determination of folic acid by a flow injection analysis assembly  

NASA Astrophysics Data System (ADS)

A flow injection (FI) method is reported for the determination of folic acid by chemiluminescence method. This method is based on the reaction of folic acid with Ru(bipy)32+ and Ce(IV) to produce chemiluminescence. The calibration curve was linear over the range of 2.5 × 10-5-3.1 × 10-7 mol/L with a detection limit of 2.3 × 10-8 mol/L (S/N = 3). The relative standard deviation of 1.0 × 10-6 mol/L folic acid was found 3.5% (n = 11). The influences of potential interfering substances were studied. The recovery was higher than 95.3%. The method was accurate, sensitive, and effective for assay of folic acid. This CL method was successfully applied to the determination of folic acid in pharmaceutical preparations. The mechanism of CL reaction was also studied.

Wabaidur, Saikh Mohammad; Alam, Seikh Mafiz; Lee, Sang Hak; Alothman, Zeid Abdullah; Eldesoky, Gaber E.

2013-03-01

370

Hydroxyl radical generation: the effect of bicarbonate, dioxygen and buffer concentration on pH-dependent chemiluminescence.  

PubMed

Enhanced chemiluminescence (CL) induced by HO* was detected using the primary enhancers luminol (Lum), isoluminol (ILum) and lucigenin (Luc), in the absence of HCO3-, at pH 9.5, 10.0 and 10.5 but not between pH 4.0 and 9.0. This was confirmed using nine different HO* generators. FeCl3/NTA/H2O2 was the only HO* generator that was able to generate singular HO* which was obtained entirely from the Fenton reaction. However, this was so only at pH 10.0 since at all other pHs multiple ROS were produced. This was confirmed by the chemical detection of the fluorescent hydroxylated product of terephthalic acid in the absence of O2. No HO* Lum-CL pH optima coincided with the O2*- mediated Lum-CL optima found at pH 8.0 and 9.0. Bicarbonate had an enhancing effect on Lum-CL which was 70-2700% at pH 10.0 for the different HO* generators. This was due to the conversion of the radical-electron from HO* to CO3*-, making CL detection more efficient since less HO* were lost initially before detection. Methyl-cypridine-luciferin analogue (MCLA) elicited CL in the pH range 4.0-10.0 with the same set of generators in the absence of HCO3-. The iron-containing generators had their different MCLA-CL optima at pH 4.5, 5.0 or 6.0, excluding those overlapping with the O2(*-)-mediated CL optima. The two copper-containing generators had optima at the same pHs, viz, 7.0 and 10.5. Again, FeCl3/NTA/H2O2 was the only HO* generator able to produce singular HO* by the Fenton reaction. However, whereas Lum-CL was able to detect singular HO* only at pH 10.0, MCLA-CL detected it at pH 5.0 and 5.5. Therefore, MCLA is the most suitable CL enhancer for physiological assessments since it is the most sensitive enhancer and has HO* CL optima nearer to physiological pH than the other probes. The HCO3- enhancement of MCLA-CL was even greater than that of Lum-CL, since increases of 114-fold and 37-fold, respectively, were obtained at these HO*-specific pH optima for FeCl3/NTA/H2O2. Therefore, bicarbonate concentration is as important a parameter as pH when the enhanced CL of a non-cellular system is determined. Hydrogen peroxide was not able to elicit CL directly but, due to trace metal contamination, it produced artifactual CL due to HO* formation. High H2O2 levels, which prevent spontaneous O2*- dismutation, helped to establish the overlapping pH optima of CL mediated by O2*- and HO* which were artifactually produced either by O2*- via H2O2 and trace metals or by perferryl intermediates, respectively. Due to spontaneous dismutation to H2O2, only 22% of the O2*- produced by HX/XO could be detected by enhanced CL. PMID:11450981

Oosthuizen, M M; Greyling, D

2001-01-01

371

Egg-Citing! Isolation of Protoporphyrin IX from Brown Eggshells and Its Detection by Optical Spectroscopy and Chemiluminescence  

ERIC Educational Resources Information Center

|A simple and cost-effective laboratory experiment is described that extracts protoporphyrin IX from brown eggshells. The porphyrin is characterized by UV-vis and fluorescence spectroscopy. A chemiluminescence reaction (peroxyoxalate ester fragmentation) is performed that emits light in the UV region. When the porphyrin extract is added as a fluor…

Dean, Michelle L.; Miller, Tyson A.; Bruckner, Christian

2011-01-01

372

A direct chemiluminescence method for the determination of nucleic acids using Ru(phen)32+–Ce(IV) system  

Microsoft Academic Search

A direct chemiluminescence method for the determination of nucleic acids has been developed based on the enhancement of\\u000a nucleic acids on the chemiluminescence light emission of the reaction between Ru(phen)3\\u000a \\u000a 2+(phen = 1,10-phenanthroline) and Ce(IV). Under the optimum conditions, the calibration graphs are linear in the range of 5.0\\u000a × 10–8–5.0 × 10–5 g\\/mL for calf thymus DNA, 8.0 ×

H.-Y. Han; Z.-K. He; Yun-E. Zeng

1999-01-01

373

A multisyringe flow injection system with immobilized glucose oxidase based on homogeneous chemiluminescence detection  

Microsoft Academic Search

In this paper, enzyme containing reactors are for the first time implemented in the multisyringe flow injection analysis (MSFIA) technique interfaced with chemiluminescence detection for biochemical assays. The automated methodology is based on the on-line substrate conversion in an oxidase packed-bed reactor and the post-column chemiluminogenic catalysed-reaction of the generated oxidising species with an organic molecule (namely, 3-aminophthalhydrazide) in front

Mat??as Manera; Manuel Miró; José Manuel Estela; V??ctor Cerdà

2004-01-01

374

Determination of copper complexation in seawater using flow injection analysis with chemiluminescence detection  

Microsoft Academic Search

Flow injection analysis with chemiluminescence detection (FIA–CL) via the reaction of copper with 1,10-phenanthroline was used for the determination of copper complexation in seawater. A detection limit of 0.1nM Cu in undiluted seawater without sample preconcentration was obtained. Copper speciation was assessed by titrating natural seawater ligands with added copper. This method was used to measure copper complexation throughout the

Heidi Zamzow; Kenneth H Coale; Kenneth S Johnson; Carole M Sakamoto

1998-01-01

375

Chemiluminescence detection of reactive oxygen species during photodynamic therapy  

NASA Astrophysics Data System (ADS)

Photodynamic therapy (PDT) utilizes photon energy to activate a pre-administered photosensitizer drug in tissue to achieve a localized tumor control. PDT cell killing mechanism is directly related to the reactive oxygen species (ROS) produced during the photochemical reactions. Conventional PDT dosimetry evaluates distributions of the photosensitizer drug, photon propagation and absorption, and availability of molecular oxygen in the target tissue. Yet, the ultimate bullet for the damaging effect is ROS. An evaluation of ROS production during PDT should provide a more direct marker for PDT. Fluoresceinyl Cypridina Luciferin Analog (FCLA) is a chemiluminescence probe that specifically interacts with ROS (singlet oxygen and/or superoxide). The work is a preliminary investigation of the feasibility using FCLA as a means to evaluate ROS production in PDT.

Xing, Da; Qin, Yanfang; Wu, Yunxia; Zhou, Jin; Luo, Shiming; He, Yonghong; Chen, Qun

2004-06-01

376

In vivo imaging of hydrogen peroxide with chemiluminescent nanoparticles.  

PubMed

The overproduction of hydrogen peroxide is implicated in the development of numerous diseases and there is currently great interest in developing contrast agents that can image hydrogen peroxide in vivo. In this report, we demonstrate that nanoparticles formulated from peroxalate esters and fluorescent dyes can image hydrogen peroxide in vivo with high specificity and sensitivity. The peroxalate nanoparticles image hydrogen peroxide by undergoing a three-component chemiluminescent reaction between hydrogen peroxide, peroxalate esters and fluorescent dyes. The peroxalate nanoparticles have several attractive properties for in vivo imaging, such as tunable wavelength emission (460-630 nm), nanomolar sensitivity for hydrogen peroxide and excellent specificity for hydrogen peroxide over other reactive oxygen species. The peroxalate nanoparticles were capable of imaging hydrogen peroxide in the peritoneal cavity of mice during a lipopolysaccharide-induced inflammatory response. We anticipate numerous applications of peroxalate nanoparticles for in vivo imaging of hydrogen peroxide, given their high specificity and sensitivity and deep-tissue-imaging capability. PMID:17704780

Lee, Dongwon; Khaja, Sirajud; Velasquez-Castano, Juan C; Dasari, Madhuri; Sun, Carrie; Petros, John; Taylor, W Robert; Murthy, Niren

2007-08-19

377

Chemiluminescence determination of ferulic acid by flow-injection analysis using cerium(IV) sensitized by rhodamine 6G  

NASA Astrophysics Data System (ADS)

A simple, sensitive and rapid flow-injection chemiluminescence method has been developed for the determination of ferulic acid based on the chemiluminescence reaction of ferulic acid with rhodamine 6G and ceric sulfate in sulphuric acid medium. Strong chemiluminescence signal was observed when ferulic acid was injected into the acidic ceric sulfate solution in a flow-cell. The present method allowed the determination of ferulic acid in the concentration range of 8.0 × 10 -6 to 1.0 × 10 -4 mol l -1 and the detection limit for ferulic acid was 8.7 × 10 -9 mol l -1. The relative standard deviation was 2.4% for 10 replicate analyses of 1.0 × 10 -5 mol l -1 ferulic acid. The proposed method was applied to the determination of ferulic acid in Taita Beauty Essence samples with satisfactory results.

Wang, Ju Peng; Li, Nian Bing; Luo, Hong Qun

2008-11-01

378

Internal detection of surface plasmon coupled chemiluminescence during chlorination of potassium thin films.  

PubMed

The interaction of chlorine with potassium surfaces is a prototype reaction with a strong non-adiabatic energy transfer leading to exoemission and chemiluminescence. Thin film K/Ag/p-Si(111) Schottky diodes with 8 nm potassium on a 5-200 nm thick Ag layer are used as 2?-photodetectors for the chemiluminescence during chlorination of the K film at 110 K. The observed photocurrent shows a sharp maximum for small exposures and decreases gradually with the increasing chloride layer. The time dependence can be explained by the reaction kinetics, which is governed initially by second-order adsorption processes followed by an electric field-assisted diffusion. The detector current corresponds to a yield of a few percent of elementary charge per reacting chlorine molecule and is orders of magnitude larger than for external detection. The photoyield can be enhanced by increasing the Ag film thickness. For Ag films of 30 and 50 nm, the yield exhibits a maximum indicating surface plasmon coupled chemiluminescence. Surface plasmon polaritons in the Ag layer are excited by the reaction and decay radiatively into Si leading to the observed currents. A model calculation for the reverse process in attenuated total reflection is applied to explain the observed current yield maxima. PMID:23343296

Becker, Felix; Krix, David; Hagemann, Ulrich; Nienhaus, Hermann

2013-01-21

379

Novel chemiluminescence-inducing cocktails, part I: the role in light emission of combinations of luminal with SIN-1, selenite, albumin, glucose oxidase and Co2+.  

PubMed

It is known that many agents influence the capacity of cells to produce reactive oxygen species. However, assaying these agents, both those that stimulate and those that inhibit reactive oxygen production, can be complicated and time consuming. Here, a method is described in which two different cocktails are employed to stimulate luminol-dependent chemiluminescence (LDCL). These cocktails are comprised of luminol, with either sodium selenite [IV] (SEL) or tellurite [IV] (TEL) (where IV and VI refer to the 4+ or 6+ oxidation state of selenium or tellurium salts, respectively), morpholinosidonimine (SIN-1), serum albumin and Co(2+), called the SIN-1a (with selenite) and SIN1b (with tellurite) cocktails, respectively; or luminol with glucose oxidase (GO), sodium selenite [IV] and Co(2+), called the GO cocktail. The cocktails functioned best in Hank's balanced salt solution (HBSS) containing 1% glucose at pH 7.4, incubated at approximately 22 degrees C. Within 30-60 s there was a burst of luminescence, which lasted for 7-10 min. In 100% ethanol, the SIN-1 cocktails also generated LDCL to 70% of that produced in HBSS. Neither selenite [VI], seleno-cystine, seleno-methionine, nor the selenium-containing drug, ebselen, could replace SEL. Moreover, the effects of the NO-donor, SIN-1, could not be replicated by the oxyradical generators, xanthine-xanthine oxidase or hypochlorous acid. Only low levels of luminescence were generated by combinations of the peroxyl radical generator, 2,2'-azobis-2-amidinopropane dihydrochloride (AAPH) with either SEL or TEL. It is suggested that light emission induced by the SIN1 cocktail results from the oxidation of SEL [IV] to the [VI] state, possibly due to the generation of mixtures of superoxide, peroxide, peroxynitrite and also of unidentified oxidant species, catalyzed by CoCo(2+). However, the involvement of hydroxyl radicals in LDCL could not be confirmed by use of either dimethyl thiourea or by electron spin resonance (ESR). LDCL induced by the two cocktails is strongly reduced by phosphates, EDTA, deferoxamine, CuCo(2+), MnCo(2+), as well as by the "classical" antioxidants superoxide dismutase (SOD), ascorbate, vitamin E, uric acid or thiols. It is suggested that these chemiluminescence cocktail systems can be used to determine the total anti-oxidant capacities of biological fluids and commercially available anti-oxidants. PMID:15901411

Ginsburg, Isaac; Sadovnic, Milu; Oron, Miriam; Kohen, Ron

2004-01-01

380

Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore--role of degranulation and myeloperoxidase  

Microsoft Academic Search

Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 microgram\\/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 micrograms\\/ml). The additional application of 0.1 microM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the

M. Suematsu; C. Oshio; S. Miura; M. Suzuki; S. Houzawa; M. Tsuchiya

1988-01-01

381

Hot Electron-Induced Electrogenerated Chemiluminescence  

Microsoft Academic Search

\\u000a In this chapter, we discuss the basics of cathodic hot electron-induced electrogenerated chemiluminescence (HECL). In the\\u000a applications of HECL, we discuss, e.g., the usable electrode materials and their advantages as well as the applicable solution\\u000a conditions in aqueous media. We also summarize the luminophore types excitable by this method and their usability as labels\\u000a in practical bioaffinity assay applications.

Johanna Suomi; Sakari Kulmala

382

Chemiluminescent ion-molecule reactions: Rotational-vibrational population distributions of CH+(A 1Pi) and CD+(A 1Pi) from C++H2 and C++D2 collisions  

Microsoft Academic Search

Optical emission in the 3000-5000 A˚ range was observed as a result of low-energy (3-8 eVCM) ion-molecule reactions of C+ ions with H2 and D2 molecules. Most of the emission is due to (A 1Pi-->X 1Sigma+) transitions of CH+ and CD+, which were each studied with 6 A˚ and 20 A˚ FWHM optical resolution. Rotational lines of much higher J

I. Kusunoki; Ch. Ottinger

1979-01-01

383

Chemiluminescent ion--molecule reactions: Rotational--vibrational population distributions of CH\\/sup +\\/(A ¹Pi) and CD\\/sup +\\/(A ¹Pi) from C\\/sup +\\/+Hâ and C\\/sup +\\/+Dâ collisions  

Microsoft Academic Search

Optical emission in the 3000--5000 A range was observed as a result of low-energy (3--8 eV\\/sub CM\\/) ion--molecule reactions of C\\/sup +\\/ ions with Hâ and Dâ molecules. Most of the emission is due to (A ¹Pi..-->..X ¹..sigma..\\/sup +\\/) transitions of CH\\/sup +\\/ and CD\\/sup +\\/, which were each studied with 6 A and 20 A FWHM optical resolution. Rotational

I. Kusunoki; C. Ottinger

1979-01-01

384

Native vs photoinduced chemiluminescence in dimethoate determination.  

PubMed

The determination of dimethoate using either its native chemiluminescent (CL) properties or its photoinduced chemiluminescence obtained by irradiation with a 15 W low-pressure mercury lamp was studied. Thereby, two flow injection systems (FIA) with and without irradiation were exhaustively optimized and their analytical characteristics studied. Better sensitivity and selectivity was found in absence of irradiation, due to the enhancing effect of hexadecylpyridinium chloride (HPC), which acted as a sensitizer. In the developed FIA-CL system, the alkaline hydrolysis of dimethoate with NaOH was performed on-line in presence of HPC. The oxidation of the product of hydrolysis with Ce(IV) in hydrochloric medium induced chemiluminescence. The method provided a limit of detection of only 0.05 ng mL(-1) without any pre-treatment. However, the combination with solid phase extraction allowed the removal of some potential interferents as well as the preconcentration of the pesticide. Finally, the developed method was successfully applied to natural waters with recoveries between 95 and 108%. PMID:22123115

Catalá-Icardo, M; López-Paz, J L; Choves-Barón, C; Peña-Bádena, A

2011-10-28

385

Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence.  

PubMed Central

A method for monitoring formation of latex particle pairs by chemiluminescence is described. Molecular oxygen is excited by a photosensitizer and an antenna dye that are dissolved in one of the particles. 1 delta gO2 diffuses to the second particle and initiates a high quantum yield chemiluminescent reaction of an olefin that is dissolved in it. The efficiency of 1 delta gO2 transfer between particles is approximately 3.5%. The technique permits real-time measurement of particle binding kinetics. Second-order rate constants increase with the number of receptor binding sites on the particles and approach diffusion control. By using antibody-coated particles, a homogeneous immunoassay capable of detecting approximately 4 amol of thyroid-stimulating hormone in 12 min was demonstrated. Single molecules of analyte produce particle heterodimers that are detected even when no larger aggregates are formed.

Ullman, E F; Kirakossian, H; Singh, S; Wu, Z P; Irvin, B R; Pease, J S; Switchenko, A C; Irvine, J D; Dafforn, A; Skold, C N

1994-01-01

386

Flow injection-chemiluminescence determination of dopamine using potassium permanganate and formaldehyde system  

NASA Astrophysics Data System (ADS)

A simple and sensitive flow injection-chemiluminescence method for the determination of dopamine has been proposed. The method is based on the enhancing effect of dopamine on the chemiluminescence emission generated by the reaction of potassium permanganate with formaldehyde in an acidic medium. The proposed procedure allows the determination of dopamine over the concentration range of 3.1 × 10-8-1.7 × 10-5 mol/L and with a detection limit of 1.0 × 10-8 mol/L. The linear regression equation was F = 44.4912 + 1.07 × 109 ? C (correlation coefficient, r2 = 0.9998). The relative standard deviation is 2.1% for the determination of 1.0 × 10-8 mol/L dopamine (n = 11). The method was successfully applied to the determination of dopamine in pharmaceutical preparation with satisfactory results. The recoveries were found in the range of 96.5-101.3%.

Wabaidur, Saikh Mohammad; Alothman, Zeid Abdullah; Alam, Seikh Mafiz; Lee, Sang Hak

2012-10-01

387

[Carbamide peroxide as source of hydrogen peroxide for the luminol application at crime scenes].  

PubMed

The solution of hydrogen peroxide is a critical ingredient of the Weber luminol application for blood detection at the crime scene. An ideal alternative to the unstable hydrogen peroxide is a solid compound which is easy to transport, stable and quick to solve in water at the crime scene. Carbamide peroxide (urea peroxide) is one of these solid hydrogen peroxide carriers which is easy to obtain as one gram tablets. At dry conditions it is stable over a long period at room temperature and even for a short time at higher temperatures. But at 70 degrees C (180 degrees F) the tablets go out of shape and cake after one hour. In the application of luminol there are no differences between the use of hydrogen peroxide and carbamide peroxide. PMID:19432086

Schwarz, Lothar; Hermanowski, Mona-Lena

388

Chemiluminescence evaluation of oxidative damage to biomolecules induced by singlet oxygen and the protective effects of antioxidants  

Microsoft Academic Search

A chemiluminescence (CL) method was developed for the evaluation of oxidative damage to biomolecules induced by singlet oxygen (1O2) and for the evaluation of the protective effects of antioxidants. The 1O2 was generated from the reaction of H2O2+OCl?. Results showed that the CL signal from the reaction of H2O2+OCl? was weak, however, it was enhanced dose-dependently with the addition of

Yu Wenli; Zhao Yaping

2005-01-01

389

A novel approach to obtaining reliable PCR results from luminol treated bloodstains.  

PubMed

In recent years the forensic scientist has been afforded great advances in technology both in the detection of latent bloodstains and in acquiring reliable DNA typing results from very small pieces of physical evidence. Scientists are now able to detect minute quantities of latent bloodstains by utilizing the luminol reagent, oftentimes indicating that an attempt has been made to conceal any evidence of bloodshed. With the introduction of PCR based technology to the forensic arena, scientists are now routinely able to obtain DNA typing results from previously insufficient amounts of biological material, items as small as a single hair, saliva on a cigarette butt, or a bloodstain the size of a pin head. We present here a merging of these two advances coupled with a new collection medium for post luminol treated latent bloodstains. The forensic scientist is now able to routinely isolate and recover an adequate amount of DNA suitable for PCR typing at all of the Promega GenePrint PowerPlex 1.1 loci. In this study, several dilutions of latent bloodstains were prepared in an effort to simulate transferred bloodstains that are routinely encountered in a crime scene setting. The latent bloodstains were treated with luminol and subsequently collected using conventional cotton tipped swabs as well as a Puritan sponge tipped swab. PCR typing at the Promega GenePrint PowerPlex 1.1 loci was then attempted upon all dilutions of the latent bloodstains for both collection mediums. The results clearly indicate that it is now routinely possible to recover adequate amounts of DNA suitable for PCR typing upon post luminol treated bloodstains. PMID:10914590

Della Manna, A; Montpetit, S

2000-07-01

390

Electrochemiluminescence of luminol for 2,4-D optical immunosensing in a flow injection analysis system  

Microsoft Academic Search

Luminol-labelled antibodies have been prepared using glutaraldehyde as a cross-linking agent and used in a 2,4-dichlorophenoxyacetic acid (2,4-D) competitive electrochemiluminescent immunosensor. 2,4-D was covalently immobilized at a glassy carbon electrode surface, via a six-carbon spacer arm, by a novel procedure which allowed us to obtain stable immobilized antigens that could be then stored dry, used and regenerated 50 times without

Christophe A Marquette

1998-01-01

391

Luminol electrochemiluminescence for the analysis of active cholesterol at the plasma membrane in single mammalian cells.  

PubMed

A luminol electrochemiluminescence assay was reported to analyze active cholesterol at the plasma membrane in single mammalian cells. The cellular membrane cholesterol was activated by the exposure of the cells to low ionic strength buffer or the inhibition of intracellular acyl-coA/cholesterol acyltransferase (ACAT). The active membrane cholesterol was reacted with cholesterol oxidase in the solution to generate a peak concentration of hydrogen peroxide on the electrode surface, which induced a measurable luminol electrochemiluminescence. Further treatment of the active cells with mevastatin decreased the active membrane cholesterol resulting in a drop in luminance. No change in the intracellular calcium was observed in the presence of luminol and voltage, which indicated that our analysis process might not interrupt the intracellular cholesterol trafficking. Single cell analysis was performed by placing a pinhole below the electrode so that only one cell was exposed to the photomultiplier tube (PMT). Twelve single cells were analyzed individually, and a large deviation on luminance ratio observed exhibited the cell heterogeneity on the active membrane cholesterol. The smaller deviation on ACAT/HMGCoA inhibited cells than ACAT inhibited cells suggested different inhibition efficiency for sandoz 58035 and mevastatin. The new information obtained from single cell analysis might provide a new insight on the study of intracellular cholesterol trafficking. PMID:23527944

Ma, Guangzhong; Zhou, Junyu; Tian, Chunxiu; Jiang, Dechen; Fang, Danjun; Chen, Hongyuan

2013-04-04

392

A study of common interferences with the forensic luminol test for blood.  

PubMed

A wide range of domestic and industrial substances that might be mistaken for haemoglobin in the forensic luminol test for blood were examined. The substances studied were in the categories of vegetable or fruit pulps and juices; domestic and commercial oils; cleaning agents; an insecticide; and various glues, paints and varnishes. A significant number of substances in each category gave luminescence intensities that were comparable with the intensities of undiluted haemoglobin, when sprayed with the standard forensic solution containing aqueous alkaline luminol and sodium perborate. In these cases the substance could be easily mistaken for blood when the luminol test is used, but in the remaining cases the luminescence intensity was so weak that it is unlikely that a false-positive test would be obtained. In a few cases the brightly emitting substance could be distinguished from blood by a small but detectable shift of the peak emission wavelength. The results indicated that particular care should be taken to avoid interferences when a crime scene is contaminated with parsnip, turnip or horseradish, and when surfaces coated with enamel paint are involved. To a lesser extent, some care should be taken when surfaces covered with terracotta or ceramic tiles, polyurethane varnishes or jute and sisal matting are involved. PMID:11512147

Quickenden, T I; Creamer, J I

393

Paper-based chromatographic chemiluminescence chip for the detection of dichlorvos in vegetables.  

PubMed

Paper chromatography was a big breakthrough in the early of 20th century but it is rarely used due to the long separation time and the diffusion on the sample spots. In this work, for the first time, a paper-based chemiluminescence (CL) analytical device combined with paper chromatography was developed for the determination of dichlorvos (DDV) in vegetables without complicated sample pretreatment. The paper chromatography separation procedure can be accomplished in 12min on a paper support (0.8×7.0cm(2)) by using 5µL sample spotted on it. After sample developing, the detection area (0.8×1.0cm(2)) was cut and inserted between two layers of water-impermeable single-sided adhesive tapes. The paper-based chip was made by attaching the middle layer of paper onto the bottom layer. Then it was covered by another tape layer, which was patterned by the cutting method to form a square hole (0.8×1.0cm(2)) in it. 10?L mixed solution of luminol and H2O2 was dropped on the detection area to produce CL. A linear relationship was obtained between the CL intensity and the concentrations of DDV in the range between 10.0ngmL(-1) and 1.0?gmL(-1)and the detection limit was 3.6ngmL(-1). Water-soluble metal ions and vitamins can be developed at different spatial locations relative to DDV, eliminating interference with DDV during detection. The paper-based chromatographic chip can be successfully used for the determination of DDV without complicated sample preparation in vegetables. This study should, therefore, be suitable for rapid and sensitive detection of trace levels of organophosphate pesticides in environmental and food samples. PMID:24021659

Liu, Wei; Kou, Juan; Xing, Huizhong; Li, Baoxin

2013-08-24

394

Field-flow fractionation of cells with chemiluminescence detection  

Microsoft Academic Search

Field-flow fractionation is a separation technique characterized by a retention mechanism which makes it suitable for sorting cells over a short analysis time, with low sample carry-over and preserving cell viability. Thanks to its high sensitivity, chemiluminescence detection is suitable for the quantification of just a few cells expressing chemiluminescence or bioluminescence. In this work, different formats for coupling gravitational

Dora Melucci; Barbara Roda; Andrea Zattoni; Sonia Casolari; Pierluigi Reschiglian; Aldo Roda

2004-01-01

395

Molecularly imprinted polymer grafted paper-based multi-disk micro-disk plate for chemiluminescence detection of pesticide.  

PubMed

The detection of pesticides has attracted considerable attention in numerous fields, such as environmental monitoring and food safety. Although traditional sensors for pesticides have been widely explored due to their high sensitivity and specificity, it is still challenging to develop a low-cost, portable, fast, and easy-to-use detection method for the public use at home or in the field. To address these challenges, herein, we report a novel paper-based molecular imprinted polymer (MIP)-grafted multi-disk micro-disk plate (P-MIP-MMP) for sensitive and specific chemiluminescence (CL) detection of pesticides through an indirect competitive assay using 2,4-dichlorophenoxyacetic acid (2,4-D) as a proof-of-concept analyte. The MIP-grafted paper disks were prepared by a simple in situ polymerization of MIP layer on the surface of cellulose fibers in paper. The quantification mechanism of this P-MIP-MMP is based on a competition between free 2,4-D and tobacco peroxidase (TOP) labeled 2,4-D and the enzyme catalyzed CL emission from the luminol-TOP-H2O2 CL system. At optimal conditions, this P-MIP-MMP can detect 2,4-D at the concentration of femtomolar level. This approach provided a powerful protocol for simple, low-cost, rapid, and high-throughput detection of pesticides in real samples with satisfactory results for use in areas such as food inspection and environmental monitoring. PMID:23871875

Wang, Shoumei; Ge, Lei; Li, Long; Yan, Mei; Ge, Shenguang; Yu, Jinghua

2013-07-05

396

Sensitive determination of epinephrine in pharmaceutical preparation by flow injection coupled with chemiluminescence detection and mechanism study.  

PubMed

A novel, rapid and sensitive method was described for the determination of epinephrine (EP) using flow injection analysis coupled with chemiluminescence (CL) detection, which based on EP enhanced the weak CL emission of luminol-KIO(4) system in NaOH solution. Parameters affecting the CL intensity and reproducibility were optimized systematically. Under the optimized experiment conditions, the net CL intensity was proportional to the concentration of EP in the range of 5.0 × 10(-8) to 1.5 × 10(-6) ?mol/L with a detection limit of 1.9 × 10(-9) ?mol/L. The relative standard deviation (RSD) was found to be 0.7% for 13 replicate determinations of 3.0 × 10(-7) ?mol/L EP. The applicability of the proposed method was illustrated in the determination of EP in pharmaceutical preparation. The recoveries of EP at different levels in EP hydrochloride injection were between 95.4 and 104.7%. One assay procedure takes only 27?s, and the sampling rate was calculated about to be 130 samples/h. The possible mechanism of the enhanced CL intensity was studied by examining CL spectra and UV-vis spectra. PMID:20017128

Liu, Yanming; Liu, Zhuanli; Shi, Yanmei

2009-12-16

397

Hydroperoxide-induced chemiluminescence of the perfused lung.  

PubMed Central

Light-emission of the perfused lung is induced by t-butyl hydroperoxide, giving chemiluminescence yields that oscillate between 800 and 1500 counts/s depending on the site and position of the lung. The response of the perfused lung to infusion with different hydroperoxides gives a pattern similar to that observed with the liver microsomal fraction; ethyl hydroperoxide shows a much higher chemiluminescence yield than the tertiary (t-butyl and cumene)hydroperoxides. Alveolar oedema affected the light-emission of the perfused lung depending on the time at which oedema developed, decreasing light emission on infusion of hydroperoxide in the oedematous lung and increasing it when oedema appeared after the maximal chemiluminescence yield was already achieved. Paraquat, administered in vivo, augmented light-emission by approximately 2-fold. The effect of paraquat was a time-dependent process. Lung chemiluminescence, compared with liver chemiluminescence, needed higher hydroperoxide concentration to induce light-emission.

Cadenas, E; Arad, I D; Fisher, A B; Boveris, A; Chance, B

1980-01-01

398

Real-time monitoring of singlet oxygen in photodynamic therapy with chemiluminescence  

NASA Astrophysics Data System (ADS)

Photodynamic therapy (PDT) is a cancer therapy most of which using light excites sensitizer mainly to produce singlet oxygen (1O2) to kill tumor cells by oxidation reaction. Monitoring the singlet oxygen production is an important task for getting more useful dosage information in photodynamic therapy to enhance the effect. In order to monitor singlet oxygen in PDT, the Chemiluminescence (CL) probe, which could react with singlet oxygen and emit photons, was selected and employed on mice to produce CL. The CL was collected and recorded by a single photon detection system in real time. The results showed that the signal intensity was high and indicated that the chemiluminescence could measure singlet oxygen in vivo sensitively. And during photodynamic therapy the CL signal dropped gradually. Different therapy dosages had different decay life. Any of the decay had two different parts: the rapid component and the slow component. During PDT, reactive oxygen would oxidize biomolecules of tissue, and oxygen was consumed. It would cause a rapid component; by combining with chemiluminescence and fluorescence detection technique, the first-order elimination coefficient of tissue was proved to be degressive during PDT. We deduced that the damaged vascular in PDT would provide littler oxygen and tissue hypoxia was more severely. It may quicken CL decay and caused the slow component. In conclusion, the results proved that monitoring 1O2 by CL could give useful information not only to evaluate the effect of PDT but also to judge the tissue oxygen depletion.

Wei, Yan Chun; Yang, Li Yong; Song, Jia Xing

2008-12-01

399

Chemiluminescence of BO{sub 2} to map the creation of thermal NO in flames  

SciTech Connect

The aim of this study is to detect and map the local conditions that generate thermal NO in flames. According to the Zeldovich mechanism, the formation of NO comes from the local conjunction of a high concentration of atomic oxygen and a temperature above a critical high level imposed by the high activation energy of the rate-limiting reaction. The green light emitted when a flame is seeded with boron salts is a chemiluminescence from the BO{sup *}{sub 2} that is chemically formed in its excited state when BO reacts with atomic oxygen. As the rate of this oxidation is also strongly increasing with temperature, the chemiluminescence of BO{sub 2} depends on the concentration of atomic oxygen and on the temperature in a way similar to the formation rate of thermal NO. This double analogy suggests the possibility of an experimental in situ simulation of the formation rate of thermal NO or at least the use of the chemiluminescence of BO{sub 2} to map the sites where thermal NO is being created. Spectroscopic experiments and comparisons with numerical simulations have been performed to test the feasibility of this technique in laminar premixed and diffusion methane/air flames. The agreement is good except in the burnt gases of fuel-rich flames. Imaging strategies with different spectral filters have been developed in the same flames to overcome the problem of interference from soot radiation in diffusion flames. (author)

Maligne, D. [CORIA CNRS UMR6614, Site Universitaire du Madrillet, BP12, 76801 St Etienne du Rouvray Cedex (France)]|[Renault s.a.s., 1 allee Cornuel, 91510 Lardy (France)]|[IFP, 1 et 4 avenue de Bois-Preau, 92852 Rueil-Malmaison Cedex (France); Cessou, A.; Stepowski, D. [CORIA CNRS UMR6614, Site Universitaire du Madrillet, BP12, 76801 St Etienne du Rouvray Cedex (France)

2009-02-15

400

Observation of Individual Chemical Reactions in Solution  

Microsoft Academic Search

Discrete chemical reaction events occurring in solution have been observed by single photon detection of a bimolecular, chemiluminescent reaction. The reactants were generated from 9,10-diphenylanthracene in acetonitrile with potential pulses applied to an ultramicroelectrode. Electrogenerated radical ions of opposite sign react to yield the excited singlet state of the parent compound. The chemical reactions were restricted to a 20-femtoliter volume

Maryanne M. Collinson; R. Mark Wightman

1995-01-01

401

Chemiluminescence detection of proteins from single cells.  

PubMed Central

The analysis of proteins from single cells requires techniques of supreme sensitivity. Although radiochemical procedures are capable of detecting small amounts of electrophoretically separated proteins, their sensitivity falls short of that required for routine detection of minor components of single cells. Utilizing the avidin-biotin interaction and the alkaline phosphatase substrate 3-(4-methoxyspiro[1,2-dioxetane-3,2'- tricyclo-[3.3.1.1(3,7)]decan]-4-yl)phenyl phosphate (AMPPD), we have developed an alternative, chemiluminescence-based method for protein detection whose sensitivity exceeds that of other methods. Applying this method to a purified protein, we could detect as little as 63 fg (0.9 amol) of biotinylated bovine serum albumin. The sensitivity of the method was demonstrated by the detection of proteins from individual photoreceptor outer segments, including proteins constituting approximately 1% of the total. Chemiluminescence detection also proved extremely sensitive for immunoblotting: a comparison of five methods for detection of antibody-antigen interactions showed that the AMPPD technique was more sensitive than detection with a colorimetric alkaline phosphatase substrate, 125I-labeled protein A, 125I-labeled anti-mouse IgG, or colloidal gold-conjugated anti-mouse IgG. Images

Gillespie, P G; Hudspeth, A J

1991-01-01

402

Imaging of DNA sequences with chemiluminescence  

SciTech Connect

We have coupled a chemiluminescent detection method that uses an alkaline phosphatase label to the genomic DNA sequencing protocol of Church and Gilbert . Images of sequence ladders are obtained on x-ray film with exposure times of less than 30 min, as compared to 40 h required for a similar exposure with a 32P-labeled oligomer. Chemically cleaved DNA from a sequencing gel is transferred to a nylon membrane, and specific sequence ladders are selected by hybridization to DNA oligonucleotides labeled with alkaline phosphatase or with biotin, leading directly or indirectly to deposition of enzyme. If a biotinylated probe is used, an incubation with avidin-alkaline phosphatase conjugate follows. The membrane is soaked in the chemiluminescent substrate (AMPPD) and is exposed to film. Dephosphorylation of AMPPD leads in a two-step pathway to a highly localized emission of visible light. The demonstrated shorter exposure times may improve the efficiency of a serial reprobing strategy such as the multiplex sequencing approach of Church and Kieffer-Higgins.

Tizard, R.; Cate, R.L.; Ramachandran, K.L.; Wysk, M.; Voyta, J.C.; Murphy, O.J.; Bronstein, I. (Biogen, Inc., 14 Cambridge Center, MA (USA))

1990-06-01

403

Luminol-dependent photoemission from single neutrophil stimulated by phorbol ester and calcium ionophore--role of degranulation and myeloperoxidase  

SciTech Connect

Luminol-dependent photonic burst from phorbol ester-treated single neutrophil was visually investigated by using an ultrasensitive photonic image intensifier microscope. Neutrophils stimulated by phorbol myristate acetate (0.1 microgram/ml) alone produced a negligible level of photonic activities in the presence of luminol (10 micrograms/ml). The additional application of 0.1 microM Ca2+ ionophore A23187 induced explosive changes of photonic burst corresponding to the distribution of neutrophils, and these photonic activities were gradually spread to extracellular space. Sodium azide, which prevents myeloperoxidase activity, inhibited Ca2+ ionophore-induced photonic burst from phorbol ester-treated neutrophil. These findings suggest a prerequisite role of degranulation and myeloperoxidase release in luminol-dependent photoemission from stimulated neutrophils.

Suematsu, M.; Oshio, C.; Miura, S.; Suzuki, M.; Houzawa, S.; Tsuchiya, M.

1988-08-30

404

CHEMILUMINESCENT CHEMI-IONIZATION: Ar* + Ca AND THE CaAr+ EMISSION SPECTRUM  

SciTech Connect

A flowing afterglow chemiluminescence apparatus has been used to analyze visible fluorescence in the Ar* ({sup 3}P{sub 2}{sup o}) + Ca ({sup 1}S{sub 0}) reaction. The rate constants for production of Ca{sup +} ({sup 2}P{sub 3/2}{sup o}) and Ca{sup +} ({sup 2}P{sub 1/2}{sup o}) were measured to be 1.6 x 10{sup -10} cm{sup 3}-molecule{sup -1} sec{sup -1} and 3.2 x 10{sup -11} cm{sup 3} molecule{sup -1} sec{sup -1}, respectively. These results demonstrate a transfer of the total electronic angular momentum polarization in Ar* tothe excited ion levels. The molecular band spectrum of the associative ionization product CaAr{sup +} (A{sup 2}{Pi}) was observed. Molecular fluorescence constituted 14% of the total fluorescence from all ion products. This spectrum was analyzed with a model (exp-Z4) potential, yielding, for the ground state, {Chi}{sup 2}{Sigma}{sup +}, R{sub e} = 2.8 {angstrom}, {omega}''{sub e} = 87 cm{sup -1}, and D''{sub e} = 1000 cm{sup -1}, and, for the A{sup 2}{Pi} state, R{sub e} = 2.6 {angstrom}, {omega}'{sub e} = 200 cm{sup -1}, and D'{sub e} = 4900 cm{sup -1}. The nascent internal state distribution in CaAr{sup +} is found to consist of a fairly narrow range of high vibrational levels. The analysis of spectra from chemiluminescent reaction is a well established technique for elucidating the product state distributions of elementary processes. In this paper, they use the analysis of the chemiluminescent chemi-ionization reactions between metastable argon atoms and calcium atoms to expose the dynamics of associative ionization (AI) and to measure the branching ratios for chemi-ionization into more than one product channel.

Hartman, Dennis C.; Winn, John S.

1980-09-01

405

Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction  

Microsoft Academic Search

Background: Polymorphonuclear neutrophils (PMNs) are crucial in host defense against invading microorganisms through reactive oxygen species (ROS) production. However, generated ROS released in excess into media can damage the host tissue. It is therefore essential, when exploring oxygen species production, to discriminate between its intracellular (IC) and extracellular (EC) localization. Several methods of ROS detection are commonly used. However, the

Florence Caldefie-Chézet; Stephane Walrand; Christophe Moinard; Arlette Tridon; Jacques Chassagne; Marie-Paule Vasson

2002-01-01

406

Comparison of the effects of antioxidant non-steroidal anti-inflammatory drugs against myeloperoxidase and hypochlorous acid luminol-enhanced chemiluminescence  

Microsoft Academic Search

The interaction of myeloperoxidase (MPO) with H2O2 and Cl? provides a potent antimicrobial\\/cytotoxic system for polymorphonuclear leukocytes (PMNs). MPO-related cytotoxicity may be associated with the formation of toxic oxidant MPO intermediates, HOCl, or both. MPO itself is able to oxidize drugs and cellular components. Non-steroidal anti-inflammatory drugs (NSAIDs) able to act as antioxidant free radical scavengers have recently been shown

Gary Pekoe; Knox Van Dyke; Henry Mengoli; David Peden; Denis English

1982-01-01

407

In vitro study of the antioxidant properties of nimesulide and 4OH nimesulide: effects on HRP- and luminol-dependent chemiluminescence produced by human chondrocytes  

Microsoft Academic Search

Objectives Reactive oxygen species (ROS) are now recognized to play an important role in the pathogenesis of rheumatic diseases and constitute an interesting therapeutic target for drugs. This in vitro study was designed to evaluate the antioxidant properties of nimesulide (NIM), a nonsteroidal antiinflammatory drug of the sulfonanilide class, and its main metabolite 4-OH nimesulide (4-OHNIM).Methods The scavenging effects of

S. X Zheng; A Mouithys-Mickalad; G. P Deby-Dupont; C. M.-T Deby; A. P Maroulis; A. H Labasse; M. L Lamy; J.-M. R Crielaard; J.-Y. L Reginster; Y. E Henrotin

2000-01-01

408

Immunomodulation by exogenous surfactant: effect on TNF-? secretion and luminol-enhanced chemiluminescence activity by murine macrophages stimulated with group B streptococci  

Microsoft Academic Search

Group B streptococci (GBS) are important pathogens in neonatal sepsis and pneumonia. GBS stimulate alveolar macrophages to produce inflammatory cytokines and free oxygen radicals, which can damage the lungs. In several studies, use of exogenous surfactant in term babies has improved outcome related to sepsis and respiratory failure. The role(s) of exogenous surfactant in modulating the inflammatory response produced by

Ajay J. Talati; Dennis T. Crouse; B. Keith English; Cynthia Newman; Lisa Harrison; Elizabeth Meals

2001-01-01

409

On the interaction of luminol with human serum albumin: Nature and thermodynamics of ligand binding  

NASA Astrophysics Data System (ADS)

The mechanism and thermodynamic parameters for the binding of luminol (LH 2) with human serum albumin was explored by steady state and picosecond time-resolved fluorescence spectroscopy. It was shown that out of two possible LH 2 conformers present is solution, only one is accessible for binding with HSA. The thermodynamic parameters like enthalpy (? H) and entropy (? S) change corresponding to the ligand binding process were also estimated by performing the experiment at different temperatures. The ligand replacement experiment with bilirubin confirms that LH 2 binds into the sub-domain IIA of the protein.

Moyon, N. Shaemningwar; Mitra, Sivaprasad

2010-09-01

410

Simple LC Method with Chemiluminescence Detection for Simultaneous Determination of Arbutin and l Ascorbic Acid in Whitening Cosmetics  

Microsoft Academic Search

Simultaneous determination of arbutin (ART) and l-ascorbic acid (AA) by HPLC with chemiluminescence detection is proposed for the first time. This method is based on the CL\\u000a reaction of acidic potassium permanganate with ART and AA in the presence of formaldehyde as enhancer. The separation was\\u000a performed on a C18 column with a 90:10 (v\\/v) mixture of 0.02 M phosphate buffer

Yue Wei; Zhujun Zhang; Yantu Zhang; Yonghua Sun

2007-01-01

411

A Novel Captopril Chemiluminescence System for Determination of Copper(II) in Human Hair and Cereal Flours  

Microsoft Academic Search

A novel chemiluminescence (CL) reaction, captopril–H2O2, for determination of Cu(II) at nanogram per milliliter level in batch-type system has been described. The method relies\\u000a on the catalytic effect of Cu(II) on the oxidation of captopril with hydrogen peroxide in alkaline medium. The optimization\\u000a step was performed using univariate methodology and the factors studied were: pH and concentrations of the utilized

M. H. Sorouraddin; M. Iranifam; A. Imani-Nabiyyi

2009-01-01

412

Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay  

PubMed Central

The oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti), titanium alloy (Ti6Al4V), and stainless steel 316L (SS) in particulate form with <20??m in size were used. The effect of particulate materials opsonisation on the upregulation of the respiratory burst production by blood cells was also assessed. The largest chemiluminescence response was achieved after simultaneous injection of the stimulants fMLP+PMA. Moreover, Ti and SS induced a greater inflammatory reaction compared to Ti6Al4V, since the respiratory burst mounted was higher for both materials after opsonisation treatment. These results suggest that in vitro chemiluminescence response and respiratory burst measurements proved to be composition and treatment dependent.

P. Cachinho, Sandra C.; Pu, Fanrong

2012-01-01

413

Free Radical Production in Immune Cell Systems Induced by Ti, Ti6Al4V and SS Assessed by Chemiluminescence Probe Pholasin Assay.  

PubMed

The oxidative burst of human blood cells in the presence of different metal materials was investigated using chemiluminescence assay. Commercial pure titanium (Ti), titanium alloy (Ti6Al4V), and stainless steel 316L (SS) in particulate form with <20??m in size were used. The effect of particulate materials opsonisation on the upregulation of the respiratory burst production by blood cells was also assessed. The largest chemiluminescence response was achieved after simultaneous injection of the stimulants fMLP+PMA. Moreover, Ti and SS induced a greater inflammatory reaction compared to Ti6Al4V, since the respiratory burst mounted was higher for both materials after opsonisation treatment. These results suggest that in vitro chemiluminescence response and respiratory burst measurements proved to be composition and treatment dependent. PMID:22778739

P Cachinho, Sandra C; Pu, Fanrong

2012-06-19

414

Sulfur Chemiluminescence Detection Compared to Sulfur Flame Photometric Detection.  

National Technical Information Service (NTIS)

Detection of compounds containing sulfur heteratom is traditionally accomplished using flame photometric detection (FPD). Sulfur chemiluminescence detection (SCD) is an alternative to FPD. This report compares the FPD with two SCD configurations using var...

M. W. Elizy L. G. Janes

1998-01-01

415

Oscillation chemiluminescence in liquid phase oxidation of organic compounds  

Microsoft Academic Search

It is for the first time that distinct chemiluminescence oscillations have been observed in the liquid phase oxidation for the system: oxidative substrate — thermal initiator — photosensitizer — O2. Oscillations and their parameters are dependent on the experimental conditions.

I. R. Akhunov; V. D. Komissarov

1986-01-01

416

Determination of the oxidative burst chemiluminescent response of avian and murine-derived macrophages versus corresponding cell lines in relation to stimulation with Salmonella serotypes.  

PubMed

In contrast to mammalian systems, avian species lack a resident or harvestable macrophage population in the abdominal exudate. Peritoneal macrophages in the chicken can be elicited if an inflammatory agent such as sephadex is injected. This study examines the kinetics of different macrophage populations, derived by different methods of isolation and from different hosts, with respect to the elicited oxidative burst upon infection with host-adapted Salmonella serotypes. The nature of the oxidative burst elicited by murine and avian-derived and cell line macrophages was determined after stimulation with phorbol myristate (PMA), zymosan A, and Salmonella serotypes. Both murine and chicken peritoneal macrophages, chicken blood monocytes and corresponding cell lines, J774A.1 and HD-11, were unable to produce a detectable chemiluminescent (CL) response after interaction with Salmonella using the luminescent probe luminol. However, both PMA and zymosan A induced a CL response in all cell types, with PMA eliciting a higher and earlier peak response (pkH) than zymosan A. Lucigenin-enhanced CL in both murine and chicken macrophages was achieved with PMA, zymosan A and Salmonella serotypes. In this case, zymosan A induced higher responses than PMA. In the peritoneal macrophages of both hosts, there were no significant differences in the oxidative burst induced by the different Salmonella serotypes. However, the J774A.1 (murine) cells demonstrated significant differences, with S. enterica serotype Choleraesuis (S. choleraesuis and S. gallinarum producing the highest response. In the HD-11 (chicken) cells, S. choleraesuis and S. dublin elicited the higher CL. With both cell lines, S. abortusovis failed to induce an appreciable CL response. In these experiments it was demonstrated that oxidative burst was not detectable in monocytes/macrophage populations using luminol, which suggests a link to the lack of a myeloperoxidase system in these cells. Lucigenin-enhanced CL appeared independent from the myeloperoxidase system, indicating production of another oxidative species compared with luminol. No discernable effect of host specificity with regard to Salmonella serotype and respective host was seen in host-derived or cell line macrophages, and cell line macrophages displayed altered functional characteristics with regard to oxidative burst in comparison with their primary counterparts. PMID:11457481

Chadfield, M; Olsen, J

2001-08-10

417

Chemiluminescence measurement of atmospheric ozone with an oil-coated paper filter  

SciTech Connect

Chemiluminescent reaction between peroxyacetyl nitrate and ozone with triethyl amine (TEA) vapor can be used to monitor atmospheric concentration. Further studies questioning the stability of TEA led to the discovery of chemiluminescence in the reaction of ozone with lubricating oil-coated cellulose paper filters. A prototype atmospheric monitor was constructed to test this phenomenon. For highest sensitivity, pressure in the reaction vessel and sample gas flow rate are 460 torr and 1.2 1/min. Blank values for photon counting were obtained by ozonizing synthetic dry air. The corrected spectrum of the light from the reactive impregnated filter was obtained in the 300 to 600 nm range. Two peaks are visible at 400 and 500 nm. With the use of an optimum nozzle, the reliability of the measurement was within 3%. The responses are linear with the ozone concentration varying between 0 and 0.19 ppm. The sensitivity of the detector appears to be satisfactory for use as an ozone monitor in the atmosphere.

Chisaka, F.; Yanagihara, S.

1982-05-01

418

Chemiluminescence Determination of Phentolamine Based on Potassium Permanganate Sensitizedby Formaldehyde  

Microsoft Academic Search

A new chemiluminescent method was proposed for the determination of phentolamine, which was based on the enhancement effect of formaldehyde on the weak chemiluminescence produced by permanganate oxidation on phentolamine in an acidic media. In optimum conditions, the proposed method allowed the determination of phentolamine in the range 0.01–5.0 µg\\/mL, with a detection limit of 5 ng\\/mL. The relative standard deviation was

Jin Pan; Yuming Huang

2004-01-01

419

Coulometric microflow titrations with chemiluminescent and amperometric equivalence point detection  

Microsoft Academic Search

A coulometric flow titration with electrogenerated bromine was combined with chemiluminescent and amperometric flow detection to determine low concentrations of hydrazine and ammonium. Hydrazine can be precisely titrated in the range between 2?M and 1.1mM with chemiluminescence detection of the equivalence points with relative standard deviations between 1.9 and 0.4% (n=5, ?=0.05). 60nM is the lowest concentration of hydrazine, which

Z. K. He; B. Fuhrmann; U. Spohn

2000-01-01

420

Field evaluation of the sulfur chemiluminescence detector  

SciTech Connect

A field evaluation of the sulfur chemiluminescence detector (SCD) as a real-time total atmospheric sulfur detector is presented. The SCD was installed in a monitoring trailer along with a flame photometric detector (FPD), fluorescent SO{sub 2} monitor (Fluor), and a suite of other monitoring instruments. The performance of the analyzers was compared for (1) baseline stability, (2) response stability, (3) interferences, (4) sensitive, and (5) environmental temperature effects. The SCD exhibited the best baseline stability and sensitivity, but had a drift in sensitivity larger than the other analyzers. The FPD and Fluor both showed interference effects, but none were observed for the SCD. Ambient temperature variations altered both the baseline and sensitivity of the FPD and the sensitivity of the Fluor. The SCD showed no ambient temperature dependence on either the baseline or sensitivity.

Benner, R.L.; Stedman, D.H. (Univ. of Denver, CO (USA))

1990-10-01

421

Biotechnological applications of bioluminescence and chemiluminescence.  

PubMed

Recent progress in molecular biology has made available several biotechnological tools that take advantage of the high detectability and rapidity of bioluminescence and chemiluminescence spectroscopy. These developments provide inroads to in vitro and in vivo continuous monitoring of biological processes (e.g. gene expression, protein-protein interaction and disease progression), with clinical, diagnostic and drug discovery applications. Furthermore, combining luminescent enzymes or photoproteins with biospecific recognition elements at the genetic level has led to the development of ultrasensitive and selective bioanalytical tools, such as recombinant whole-cell biosensors, immunoassays and nucleic acid hybridization assays. The high detectability of the luminescence analytical signal makes it appropriate for miniaturized bioanalytical devices (e.g. microarrays, microfluidic devices and high-density-well microtiter plates) for the high-throughput screening of genes and proteins in small sample volumes. PMID:15158059

Roda, Aldo; Pasini, Patrizia; Mirasoli, Mara; Michelini, Elisa; Guardigli, Massimo

2004-06-01

422

Kinetic-spectrometric three-dimensional chemiluminescence as an effective analytical tool. Application to the determination of benzo(a)pyrene.  

PubMed

Kinetic and spectroscopic methods were used in combination in this work to develop a new analytical tool for use in chemiluminescence detection processes. Specifically, time-resolved chemiluminescence was used jointly with a stopped-flow assembly in order to monitor the chemiluminescence produced in the oxidation of bis(2,4-dinitrophenyl)oxalate (DNPO) by hydrogen peroxide in the presence of a polycyclic aromatic hydrocarbon. Recording of successive two-dimensional spectra during the emission process and treating the acquired spectral data with dedicated software allows the obtainment of three-dimensional chemiluminescence spectra, a result of the joint use of two analytical techniques. Thus, using a flow cell specifically designed for direct coupling to the charge-coupled device (CCD) detector increases the emission intensity without the need for fibre optics. Also, using dedicated software to process the acquired two-dimensional spectra affords a comprehensive kinetic and spectroscopic characterization of the chemiluminescence signal via the three-dimensional spectrum previously obtained. The analytical potential of this new tool was assessed by application to the chemiluminescent reaction between a peroxyoxalate and an oxidant (hydrogen peroxide); the reaction is induced by benzo(a)pyrene, which was used to determine this polycyclic aromatic hydrocarbon in an organic solvent. A linear calibration graph was obtained between 0.5 and 20 mg L(-1). The limit of detection found to be 3.97 ?g L(-1) and a relative standard error of 0.64% and a relative standard deviation of 1.87% were obtained. The results reached testify to the usefulness of the proposed analytical tool for simple determinations and its potential for the resolution of complex mixtures or determinations in complex matrices. PMID:21458634

Pulgarín, José Antonio Murillo; Bermejo, Luisa F García; García, M Nieves Sánchez; Robles, Ignacio Sánchez-Ferrer

2011-03-01

423

Self-assembly of organogels via new luminol imide derivatives: diverse nanostructures and substituent chain effect  

NASA Astrophysics Data System (ADS)

Luminol is considered as an efficient sycpstem in electrochemiluminescence (ECL) measurements for the detection of hydrogen peroxide. In this paper, new luminol imide derivatives with different alkyl substituent chains were designed and synthesized. Their gelation behaviors in 26 solvents were tested as novel low molecular mass organic gelators. It was shown that the length and number of alkyl substituent chains linked to a benzene ring in gelators played a crucial role in the gelation behavior of all compounds in various organic solvents. Longer alkyl chains in molecular skeletons in present gelators are favorable for the gelation of organic solvents. Scanning electron microscope and atomic force microscope observations revealed that the gelator molecules self-assemble into different micro/nanoscale aggregates from a dot, flower, belt, rod, and lamella to wrinkle with change of solvents. Spectral studies indicated that there existed different H-bond formations and hydrophobic forces, depending on the alkyl substituent chains in molecular skeletons. The present work may give some insight to the design and characteristic of new versatile soft materials and potential ECL biosensors with special molecular structures.

Jiao, Tifeng; Huang, Qinqin; Zhang, Qingrui; Xiao, Debao; Zhou, Jingxin; Gao, Faming

2013-06-01

424

Self-assembly of organogels via new luminol imide derivatives: diverse nanostructures and substituent chain effect.  

PubMed

Luminol is considered as an efficient sycpstem in electrochemiluminescence (ECL) measurements for the detection of hydrogen peroxide. In this paper, new luminol imide derivatives with different alkyl substituent chains were designed and synthesized. Their gelation behaviors in 26 solvents were tested as novel low molecular mass organic gelators. It was shown that the length and number of alkyl substituent chains linked to a benzene ring in gelators played a crucial role in the gelation behavior of all compounds in various organic solvents. Longer alkyl chains in molecular skeletons in present gelators are favorable for the gelation of organic solvents. Scanning electron microscope and atomic force microscope observations revealed that the gelator molecules self-assemble into different micro/nanoscale aggregates from a dot, flower, belt, rod, and lamella to wrinkle with change of solvents. Spectral studies indicated that there existed different H-bond formations and hydrophobic forces, depending on the alkyl substituent chains in molecular skeletons. The present work may give some insight to the design and characteristic of new versatile soft materials and potential ECL biosensors with special molecular structures. PMID:23758979

Jiao, Tifeng; Huang, Qinqin; Zhang, Qingrui; Xiao, Debao; Zhou, Jingxin; Gao, Faming

2013-06-10

425

Differential effects of luminol, nickel, and arsenite on the rejoining of ultraviolet light and alkylation-induced DNA breaks  

SciTech Connect

When Chinese hamster ovary cells were treated with ultraviolet (UV) light or methyl methane-sulfonate (MMS), a large number of DNA strand breaks could be detected by alkaline elution. These strand breaks gradually disappeared if the treated cells were allowed to recover in a drug-free medium. The presence of nickel or arsenite during the recovery incubation retarded the disappearance of UV-induced strand breaks, whereas the disappearance of MMS-induced strand breaks was retarded by the presence of arsenite or of luminol, a new inhibit for poly(ADP-ribose) synthetase. Luminol, however, had no apparent effect on the repair of UV-induced DNA strand breaks, and nickel had no effect on the repair of MMS-induced DNA strand breaks. When UV- or MMS-treated cells were incubated in cytosine arabinofuranoside (AraC) plus hydroxyurea (HU), a large amount of low molecular weight DNA was detected by alkaline sucrose sedimentation. The molecular weight of these DNAs increased if the cells were further incubated in a drug-free medium. This rejoining of breaks in cells pretreated with UV plus AraC and HU was inhibited by nickel and by arsenite, but not by luminol. The rejoining of breaks in cells pretreated with MMS plus AraC and HU was inhibited by luminol and by arsenite, but not by nickel. These results suggest that different enzymes may be used in DNA resynthesis and/or ligation during the repairing of UV- and MMS-induced DNA strand breaks, and that nickel, luminol, and arsenite may have differential inhibitory effects on these enzymes. 29 refs., 4 figs., 1 tab.

Lee-Chen, S.F.; Yu, C.T.; Wu, D.R. [Institute of Zoology, Taipei (Taiwan, Province of China)] [and others

1994-12-31

426

Flow-injection chemiluminescence determination of diazepam by oxidation with N-bromosuccinimide.  

PubMed

A rapid and sensitive flow-injection chemiluminescence (FI-CL) method is described for the determination of diazepam based on its reaction with N-bromosuccinimide (NBS) in alkaline medium in the presence of dichlorofluorescein (DCF) as an effective energy-transfer agent. Under optimum conditions, the proposed method allowed the measurement of diazepam over the range of 2.0?×?10(-6) to 2.0?×?10(-4) ?mol/L with a detection limit of 5.0?×?10(-7) ?mol/L. The relative standard deviation for 11 parallel measurements of 2.0?×?10(-5) ?mol/L diazepam was 2.1%. The method was applied satisfactorily for the determination of diazepam in pharmaceutical preparations, and the results agree well with those obtained by spectrophotometry. The use of the proposed system for the determination of diazepam in urine and plasma samples was also tested. The possible mechanism of the chemiluminescence reaction is discussed briefly. Copyright © 2012 John Wiley & Sons, Ltd. PMID:23175271

Han, Suqin; Jia, Shize; Guo, Liang

2012-11-23

427

[Chemiluminescence of whole saliva in antioxidant treatment of prosthetic bed tissues].  

PubMed

Inflammatory reaction is always accompanied by increased intensity of free-radical oxidation, especially when the phenomena of hypoxia and microcirculatory disorders that occur during the development of side-effects of acrylic removable dentures. This study determined the effectiveness of adaptogens, antioxidants in the complex treatment of diseases of tissues prosthetic field and their influence on the processes of LPO in whole mixed unstimulated saliva. Formed in the reaction to initiate the process of oxygen radicals (OH, RO, O(2)), initiate the formation of lipid peroxide radicals RO(2) biological substrate, the recombination of which leads to the emergence of unsustainable tetroxids, which decays with the release of light quanta. This luminescence is recorded as an amplified current of the photomultiplier, the registration systems. The results suggest the intensive formation of free radicals and peroxides in diseased tissue prosthetic field. Probably the main reason for increasing free-radical oxidation is the release of peroxidase from the crumbling inflammation, phagocytes (mainly neutrophils). The process of peroxidation contributes to an increase in blood supply to inflamed tissues, leading to local enrichment of oxygen, as well as toxic effects of acrylic bases of partial and complete removable dentures in the prosthetic field of tissue. Effect of antioxidants in combination with traditional treatment in 70 patients with periodontal disease and prosthetic bed was assessed by chemiluminescence analysis of whole mixed unstimulated saliva. The level of lipid peroxidation and chemiluminescence activity exceeded the normal values in the 1,5-2 - twice before the treatment. After treatment with antioxidants, these parameters decreased and increased during remission. Thus, studies to determine the status of saliva chemiluminescence method to treat and monitor the dynamics after treatment of periodontitis tissues supporting teeth prosthetic field in the control group and the main observation, revealed the following pattern: the approach of all the indices to normal in patients with a core group, which corresponded to the clinical dynamic index parameters of periodontal tissues supporting teeth prosthetic field, and a similar core group of the positive dynamics of the intensity values of chemiluminescence-indicators in the control group up to 3 months of observation, with a significant deterioration of the same indicators at a later time dynamic monitoring. PMID:20413812

Tunian, M Iu; Lalaian, B K; Zakarian, A E; Grigorian, K L; Pogosian, G A; Egiazarian, A V

2010-03-01

428

Composite poly(dimethylsiloxane)\\/glass microfluidic system with an immobilized enzymatic particle-bed reactor and sequential sample injection for chemiluminescence determinations  

Microsoft Academic Search

A three-layer poly(dimethylsiloxane) (PDMS)\\/glass microfluidic system for performing on-chip solid-phase enzymatic reaction and chemiluminescence (CL) reaction was used for the determination of glucose as a model analyte. A novel method for the immobilization of controlled-pore-glass based reactive particles on PDMS microreactor beds was developed, producing an on-chip solid-phase reactor that featured large reactive surface and low flow impedance. Efficient mixing

Zhang-Run Xu; Zhao-Lun Fang

2004-01-01

429

Flow Injection Technique for Biochemical Analysis with Chemiluminescence Detection in Acidic Media  

PubMed Central

A review with 90 references is presented to show the development of acidic chemiluminescence methods for biochemical analysis by use of flow injection technique in the last 10 years. A brief discussion of both the chemiluminescence and flow injection technique is given. The proposed methods for biochemical analysis are described and compared according to the used chemiluminescence system.

Chen, Jing; Fang, Yanjun

2007-01-01

430

Chemiluminescence of lipid vesicles supplemented with cytochrome c and hydroperoxide.  

PubMed Central

The increase in light emission of hydroperoxide-supplemented cytochrome c observed on addition of lipid vesicles was related to the degree of unsaturation of the fatty acids of the phospholipids: dipalmitoyl phosphatidylcholine was without effect, whereas dioleoyl phosphatidylcholine and soya-bean phosphatidylcholine enhanced chemiluminescence 2- and 3-fold respectively. Effects on light-emission were similar to those on O2 uptake. The chemiluminescence of the present system was sensitive to cyanide and to the radical trap 2,5-di-t-butylquinol, indicating a catlytic activity of cytochrome c and the presence of free-radical species respectively. Lipid-vesicle enhanced chemiluminescence showed different kinetic behaviours, apparently depending on unsaturation: three phases are described for soya-bean phosphatidylcholine, whereas only one phase was present in mixtures containing dipalmitoyl and dioleoyl phospholipids. Chemiluminescence of lipid vesicles supplemented with cytochrome c and hydroperoxide showed similar kinetic patterns with H2O2 and primary (ethyl) and tertiary (t-butyl and cumene) hydroperoxides. Participation of singlet molecular oxygen, mainly on the phase III of chemiluminescence, is suggested by the increase of light-emission by 1,4-diazabicyclo[2.2.2]-octane as well as by data from spectral analysis.

Cadenas, E; Boveris, A; Chance, B

1980-01-01

431

Mechanism of neutrophil chemiluminescence induced by wheat germ agglutinin: partial characterization of the antigens recognized by wheat germ agglutinin  

SciTech Connect

Wheat germ agglutinin (WGA) stimulated neutrophils to produce significant levels of luminol-dependent chemiluminescence (CL). Since WGA is known to bind N-acetylglucosamine (GlcNAc) oligomers and N-acetylneuraminic acid (NANA), we attempted to determine which binding property of WGA is essential for induction of CL. The succinylated form of WGA (SuWGA), which is no longer able to bind NANA, was still able to induce CL. N-Acetylglucosamine at a concentration of 20 mmol/L almost completely inhibited WGA-induced CL production by neutrophils, whereas bovine submaxillary gland mucin, a potent blocker of NANA binding of WGA, failed to inhibit CL production. Lectins with the GlcNAc-binding property were examined for their ability to induce CL. Those that have higher valences and have a tendency to bind GlcNAc oligomers in the internal portion of glycoconjugates were able to induce CL, whereas those that have low valences and bind terminal GlcNAc of glycoconjugates failed to induce CL even at high concentrations. Attempts were made to characterize the neutrophil membrane proteins recognized by WGA. Glycoproteins with a molecular weight of 25,000 daltons were identified by a 50 mmol/L GlcNAc elution of WGA gels loaded with /sup 125/I-labeled neutrophil membrane proteins. Elution with 500 mumol/L GlcNAc trimer produced several glycoproteins of different molecular weights in addition to the glycoproteins of 25,000 daltons. /sup 125/I-labeled WGA and SuWGA were used for autoradiographic analysis of cell extracts of the neutrophils separated on sodium dodecyl sulfate polyacrylamide gels. WGA recognized multiple glycoproteins of different molecular weights, whereas SuWGA bound only a few of them. Glycoproteins of 25,000 daltons, probably corresponding to those identified by 50 mmol/L GlcNAc elution, were also recognized.

Ozaki, Y.; Iwata, J.; Ohashi, T.

1984-11-01

432

Highly sensitive chemiluminescence immunoassay on chitosan membrane modified paper platform using TiO2 nanoparticles/multiwalled carbon nanotubes as label.  

PubMed

A highly sensitive chemiluminescence (CL) immunoassay was incorporated into a low-cost microfluidic paper-based analytical device (?-PAD) to fabricate a facile paper-based CL immunodevice (denoted as ?-PCLI). This ?-PCLI was constructed by covalently immobilizing capture antibody on a chitosan membrane modified ?-PADs, which was developed by simple wax printing methodology. TiO2 nanoparticles coated multiwalled carbon nanotubes (TiO2/MWCNTs) were synthesized as an amplification catalyst tag to label signal antibody (Ab2). After sandwich-type immunoreactions, the TiO2/MWCNTs were captured on the surface of ?-PADs to catalyze the luminol-p-iodophenol-H2O2 CL system, which produced an enhanced CL emission. Using prostate-specific antigen as a model analyte, the approach provided a good linear response range from 0.001 to 20 ng/mL with a low detection limit of 0.8 pg/mL under optimal conditions. This ?-PCLI showed good reproducibility, selectivity and stability. The assay results of prostate-specific antigen in clinical serum samples were in good agreement with that obtained by commercially used electrochemiluminescence methods at the Cancer Research Center of Shandong Tumor Hospital (Jinan, Shandong Province, China). This ?-PCLI could be very useful to realize highly sensitive, qualitative point-of-care testing in developing or developed countries. PMID:23355319

Li, Weiping; Ge, Shenguang; Wang, Shoumei; Yan, Mei; Ge, Lei; Yu, Jinghua

2013-01-27

433

Roles of CdS quantum dots in 1,1'-oxalyldiimidazole chemiluminescence.  

PubMed

The roles of CdS nanocrystals (quantum dots) stabilized by oleic acid ligands in toluene were investigated in the 1,1'-oxalyldiimidazole chemiluminescence (ODI-CL) reaction generated under various environmental conditions. CdS excited by high-energy intermediates formed in ODI-CL reaction emitted dim CL. Also, CdS acted as a catalyst to enhance the yield of high-energy intermediates, capable of transferring energy to fluorescent molecules, in aqueous ODI-CL reactions, whereas it acted as a strong quencher, capable of inhibiting the CL emission of excited fluorescent molecules, in non-aqueous ODI-CL reactions. Based on the role of CdS in the aqueous ODI-CL reaction, the limit of detection (LOD = signal/noise = 3, 0.1 ?M) determined to quantify glucose using aqueous ODI-CL reaction in the presence of 2.75 ?M CdS was four times lower than that in the absence of CdS. The range of recovery determined in the aqueous ODI-CL reaction in the presence of CdS was 91.7-104%. We expect that the aqueous ODI-CL reaction in the presence of CdS can be applied as a highly sensitive sensor in various research fields such as bioanalytical chemistry, environmental engineering, homeland security, and toxicology. PMID:23034656

Cho, Heejin; Lee, Syungju; Lee, Ji Hoon

2012-10-04

434

Design and Fabrication of a Prototype Cold Background Experimental Reaction Chamber and Spectral Detection System.  

National Technical Information Service (NTIS)

The design and fabrication of a low temperature chemical reaction chamber was successfully completed. A spectral detection system to observe chemiluminescence in the infrared was also completed. Sample spectra taken in the finished chamber are shown to de...

W. M. Moore

1977-01-01

435

Permanganate-bromide-silver nanoparticles as a new chemiluminescence system and its application to captopril determination.  

PubMed

A novel chemiluminescence (CL) system based on the oxidation of bromide by permanganate in sulfuric acid medium is introduced. The enhancing effect of silver nanoparticles (NPs), synthesized by chemical reduction method, on this reaction was studied. It was demonstrated that spherical silver nanoparticles with average size of 18nm had a most remarkable catalytic effect on this reaction. CL emission wavelengths and UV-vis spectra were used to characterize the system and propose a possible mechanism. Furthermore, it was found that captopril inhibits the action of NPs and decreases the intensity of CL. Based on this phenomenon, a new CL method was developed for the determination of captopril in the 3.0×10(-10) to 1.0×10(-7)molL(-1) concentration range with a detection limit (3s) of 0.12nmolL(-1). The method was successfully applied to the determination of captopril in pharmaceutical formulations, human urine and serum samples. PMID:24054638

Amjadi, Mohammad; Manzoori, Jamshid L; Hassanzadeh, Javad; Sorouraddin, Mohammad H

2013-06-22

436

Measurement of Plasma Nitrite by Chemiluminescence without Interference of S-, N-nitroso and Nitrated Species  

PubMed Central

Recent studies have demonstrated that plasma nitrite (NO2-) reflects endothelial nitric oxide synthase activity and it has been proposed as a prognostic marker for cardiovascular disease. In addition, NO2- itself has been shown to have biological activities thought to be triggered by reduction back to NO in blood and tissues. The development of sensitive and reproducible methods for the quantitative determination of plasma NO2- is, therefore, of great importance. Ozone-based chemiluminescence assays have been shown to be highly sensitive for the determination of nanomolar quantities of NO and NO related species in biological fluids. We report here an improved direct chemiluminescence method for the determination of plasma NO2- without interference of other nitric oxide related species such as nitrate, S-nitrosothiols, N-nitrosamines, nitrated proteins and nitrated lipids. The method involves a reaction system consisting of glacial acetic acid and ascorbic acid in the purge vessel of the NO analyzer. Under these acidic conditions NO2- is stoichiometrically reduced to NO by ascorbic acid. Fasting human plasma NO2- values were found in the range of 56-210 nM (mean =110 ± 36 nM). This method has high sensitivity with an accuracy of 97% and high precision (C.V <10%) for determination of plasma nitrite. The present method is simple and highly specific for plasma NO2-. It is particularly suited to evaluate vasculature endothelial NO production that predicts the risks for cardiovascular disease.

Nagababu, Enika; Rifkind, Joseph M.

2009-01-01

437

Determination of oxides of nitrogen (NO/sub x/) in cigarette smoke by chemiluminescent analysis  

SciTech Connect

The successful application of a commercial chemiluminescent No/sub x/ analyzer to the determination of oxides of nitrogen in cigarette smoke is reported. Individual puffs of the smoke vapor phase are rapidly diluted in an air stream before introduction into the analyzer. This acts to both reduce quenching of the chemiluminescent response by CO/sub 2/ and to prevent side reactions of the NO/sub x/ with vapor phase organic constituents. Sweeping the dilute smoke through a reduced silver-ion exchange resin bed removed a substantial positive interference from hydrogen cyanide. A range of deliveries of 3 to 47 ..mu..mol of NO/sub x/ per cigarette was observed for nine types of experimental cigarettes. Statistically significant differences between NO/sub x/ and NO levels (NO/sub x/ - NO = NO/sub 2/) in smoke were observed in only one type of cigarette, presumably due to large cigarette-to-cigarette variability in constituent deliveries. 2 figures, 3 tables.

Jenkins, R.A.; Gill, B.E.

1980-05-01

438

The use of electrodialysis to prepare aqueous bread extracts for bromate determination by chemiluminescence.  

PubMed

A cleanup procedure based on electrodialysis is described for the preparation of aqueous bread extracts for bromate determination by chemiluminescence. The technique utilizes electrophoresis with 3 chambers separated by semipermeable membranes. The relative merits of reverse osmosis (RO), ultrafiltration, and nanofiltration membranes with various molecular weight cutoffs were evaluated. The best results were obtained with an RO membrane manufactured from thin-film (composite) polysulfone as support for polyamide. A 0.14 M sodium sulfate solution in the center or collection chamber provides optimum conductivity. Aqueous hydroxylamine sulfate (30 mM) was selected for the anode compartment as a reductant for the anode oxidation products. The constant current mode at 150 mA with a potential of ca 100 volts was used. After electrophoretic separation, the bromate concentration in the collection chamber was typically 2 to 3 times greater than the concentration in the bread extract. The chemiluminescent reaction of bromate with sulfite with hydrocortisone as the enhancer was selected for detection of bromate. The emission, with a wavelength maximum at 575 nm, was found to "glow" rather than "flash" after the reagents were mixed; therefore, it was possible to optimize the light collection period. The method was validated with a variety of commercial bread products. White bread, hot dog buns, hamburger rolls, and a multigrain bread from 7 different manufacturers were studied. PMID:16001854

Himata, Katsuichi; Warner, Charles; Currie, Douglas; Graves, Qian; Diachenko, Gregory

439

Enhanced flow-injection–chemiluminometric determination of sulphonamides by on-line photochemical reaction  

Microsoft Academic Search

The viability of the tandem photochemical reaction-chemiluminescence detection has been studied for an heterogeneous group of sulphonamides (sulphamethoxazole, sulphadiazine, sulphamerazine, sulphamethoxypyridazine, sulphacetamide, sulphadimidine, sulphanilamide, sulphathiazole and sulphaguanidine) using sulphamethoxazole (whose chemiluminescent behaviour has not been previously reported) as a test substance. The ‘on-line’ photochemical-reaction of sulphonamides provides an enhancing influence on the chemiluminometric response of the drugs during their oxidation

M. Catalá Icardo; J. V. Garc??a Mateo; M. Fernández Lozano; J. Mart??nez Calatayud

2003-01-01

440

Chemistry of Excited States.  

National Technical Information Service (NTIS)

The photoreduction of anthraquinone was studied and spectro obtained. The Photo-Fries rearrangement was studied. The chemiluminescence of luminol in dimethylsulfoxide and dimethylsulfoxide-water systems was studied. The chemiluminescence of luminol in wat...

D. M. Hercules

1973-01-01

441

Label-free genotyping of cytochrome P450 2D6*10 using ligation-mediated strand displacement amplification with DNAzyme-based chemiluminescence detection.  

PubMed

Genotyping of cytochrome P450 monooxygenase 2D6*10 (CYP2D6*10) plays an important role in pharmacogenomics, especially in clinical drug therapy of Asian populations. This work reported a novel label-free technique for genotyping of CYP2D6*10 based on ligation-mediated strand displacement amplification (SDA) with DNAzyme-based chemiluminescence detection. Discrimination of single-base mismatch is firstly accomplished using DNA ligase to generate a ligation product. The ligated product then initiates a SDA reaction to produce aptamer sequences against hemin, which can be probed by chemiluminescence detection. The proposed strategy is used for the assay of CYP2D6*10 target and the genomic DNA. The results reveal that the proposed technique displays chemiluminescence responses in linear correlation to the concentrations of DNA target within the range from 1 pM to 1 nM. A detection limit of 0.1 pM and a signal-to-background ratio of 57 are achieved. Besides such high sensitivity, the proposed CYP2D6*10 genotyping strategy also offers superb selectivity, great robustness, low cost and simplified operations due to its label-free, homogeneous, and chemiluminescence-based detection format. These advantages suggest this technique may hold considerable potential for clinical CYP2D6*10 genotyping and association studies. PMID:22123119

Wang, Hong-Qi; Wu, Zhan; Zhang, Yan; Tang, Li-Juan; Yu, Ru-Qin; Jiang, Jian-Hui

2011-11-06

442

Ortho-Substituted Polychlorinated Biphenyls Activate Respiratory Burst Measured as Luminol-Amplified Chemoluminescence in Human Granulocytes  

Microsoft Academic Search

The effect of polychlorinated biphenyls (PCBs) on the activation of respiratory burst measured as luminol-amplified chemoluminescence in human granulocytes is elucidated here. Chemoluminescence was stimulated in a concentration-dependent manner (ED50? 10 ?M) byortho-substituted PCB congeners, whilemeta- andpara-substituted congeners had no significant effect. Twoortho-substituted PCB congeners were chosen for the mechanistic studies, namely 2,2?,4,4?-TeCB and 2,2?-DCB, since they have been used

Ø. A. Voie; P. Wiik; F. Fonnum

1998-01-01

443

Ischemic Preconditioning Decreases Oxidative Stress during Reperfusion: A Chemiluminescence Study  

Microsoft Academic Search

The mechanism responsible for ischemic preconditioning (IPC) is still unknown but may involve the induction of antioxidant enzymes decreasing oxidative stress during subsequent periods of ischemia (I) and reperfusion (RP). The purpose of this study was to determine whether, in fact, an antioxidant mechanism is involved in the protection afforded by IPC. Lucigenin-enhanced chemiluminescence (LEC), a direct, continuous, nondestructive, on-line

Juan A. Crestanello; David M. Lingle; Joseph Kamelgard; John Millili; Glenn J. R. Whitman

1996-01-01

444

Chemiluminescent Emission Data For Kinetic Modeling Of Ethanol Combustion  

Microsoft Academic Search

Kinetic modeling is a powerful tool for combustion investigations and has been widely used. The validation of the model is a very important element of the work, to achieve realistic results, and it normally uses concentration profiles