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Sample records for multiphoton jaynes-cummings model

  1. Revival-collapse phenomenon in the quadrature squeezing of the multiphoton intensity-dependent Jaynes Cummings model

    NASA Astrophysics Data System (ADS)

    El-Orany, Faisal A. A.

    2006-12-01

    For the multiphoton intensity-dependent Jaynes Cummings model (JCM) described by a two-level atom interacting with a radiation field, we prove that there is a relationship between the atomic inversion and the quadrature squeezing. We give the required condition to obtain best information from this relation. Also we show that this relation is only sensitive to large values of the detuning parameter. Furthermore, we discuss briefly such relation for the off-resonance standard JCM.

  2. Evolution of the superposition of displaced number states with the two-atom multiphoton Jaynes Cummings model: interference and entanglement

    NASA Astrophysics Data System (ADS)

    El-Orany, Faisal A. A.

    2006-11-01

    In this paper, we study the evolution of two two-level atoms interacting with a single-mode quantized radiation field, namely, the two-atom multiphoton Jaynes-Cummings model (JCM). We assume that the field and the atoms are initially prepared in the superposition of displaced number states and excited atomic states, respectively. For this system, we investigate the atomic inversion, Wigner function, phase distribution and entanglement. We show that for symmetric (asymmetric) atoms, the system can generate asymmetric (symmetric) cat states at a quarter of the revival time. Furthermore, the degrees of entanglement for the field-atoms and the one-atom-remainder tangles depend on the rate of energy flow between the parties. The interference in phase space decreases the degree of entanglement in the bipartite.

  3. The revival collapse phenomenon in the quadrature field components of the two-mode multiphoton Jaynes Cummings model

    NASA Astrophysics Data System (ADS)

    El-Orany, Faisal A. A.

    2005-11-01

    In this paper we consider a system consisting of a two-level atom in an excited state interacting with two modes of a radiation field prepared initially in l-photon coherent states. This system is described by a two-mode multiphoton (i.e., k1,k2) Jaynes-Cummings model (JCM). For this system we investigate the occurrence of the revival-collapse phenomenon (RCP) in the evolution of the single-mode, two-mode, sum and difference quadrature squeezing. We show that there is a class of states for which all these types of squeezing exhibit RCP similar to that involved in the corresponding atomic inversion. Also we show numerically that the single-mode squeezing of the first mode for (k1,k2) = (3,1) provides RCP similar to that of the atomic inversion of the case (k1,k2) = (1,1); however, sum and difference squeezing give partial information on that case. Moreover, we show that single-mode, two-mode and sum squeezing for the case (k1,k2) = (2,2) provides information on the atomic inversion of the single-mode two-photon JCM. We derive the rescaled squeezing factors giving accurate information on the atomic inversion for all cases. The consequences of these results are that the homodyne and heterodyne detectors can be used to detect the RCP for the two-mode JCM.

  4. Supersymmetry in the Jaynes-Cummings model

    SciTech Connect

    Castanos, Octavio

    2013-06-12

    A review is presented of the Darboux method and its relation to the supersymmetric quantum mechanics, together with the embedding of a n-dimensional scalar Hamiltonian into a supersymmetric matrix. It is also shown that the Jaynes-Cummings model, with or without rotating wave approximation, admit a supersymmetric quantum mechanics description.

  5. Generalized Jaynes-Cummings model as a quantum search algorithm

    SciTech Connect

    Romanelli, A.

    2009-07-15

    We propose a continuous time quantum search algorithm using a generalization of the Jaynes-Cummings model. In this model the states of the atom are the elements among which the algorithm realizes the search, exciting resonances between the initial and the searched states. This algorithm behaves like Grover's algorithm; the optimal search time is proportional to the square root of the size of the search set and the probability to find the searched state oscillates periodically in time. In this frame, it is possible to reinterpret the usual Jaynes-Cummings model as a trivial case of the quantum search algorithm.

  6. Quantum entangled supercorrelated states in the Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Rajagopal, A. K.; Jensen, K. L.; Cummings, F. W.

    1999-08-01

    The regions of independent quantum states, maximally classically correlated states, and purely quantum entangled (supercorrelated) states described in a recent formulation of quantum information theory by Cerf and Adami are explored here numerically in the parameter space of the well-known exactly soluble Jaynes-Cummings model for equilibrium and nonequilibrium time-dependent ensembles.

  7. Negativity as entanglement degree of the Jaynes Cummings model

    NASA Astrophysics Data System (ADS)

    Akhtarshenas, S. J.; Farsi, M.

    2007-05-01

    In this paper, by using the notion of negativity, we study the degree of entanglement of a two-level atom interacting with a quantized radiation field, described by the Jaynes-Cummings model (JCM). We suppose that initially the field is in a pure state and the atom is in a general mixed state. In this case the negativity fully captures the entanglement of the JCM. We investigate the case that the initial state of the field is a coherent state. The influences of the detuning on the degree of entanglement are also examined.

  8. Entropy exchange and entanglement in the Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Boukobza, E.; Tannor, D. J.

    2005-06-01

    The Jaynes-Cummings model (JCM) is the simplest fully quantum model that describes the interaction between light and matter. We extend a previous analysis by Phoenix and Knight [Ann. Phys. 186, 381 (1988)] of the JCM by considering mixed states of both the light and matter. We present examples of qualitatively different entropic correlations. In particular, we explore the regime of entropy exchange between light and matter, i.e., where the rate of change of the two are anticorrelated. This behavior contrasts with the case of pure light-matter states in which the rate of change of the two entropies are positively correlated and in fact identical. We give an analytical derivation of the anticorrelation phenomenon and discuss the regime of its validity. Finally, we show a strong correlation between the region of the Bloch sphere characterized by entropy exchange and that characterized by minimal entanglement as measured by the negative eigenvalues of the partially transposed density matrix.

  9. Quantum Computation with the Jaynes-Cummings Model

    NASA Astrophysics Data System (ADS)

    Azuma, H.

    2011-09-01

    In this paper, we propose a method for building a two-qubit gate with the Jaynes-Cummings model (JCM). In our scheme, we construct a qubit from a pair of optical paths where a photon is running. Generating Knill, Laflamme and Milburn's nonlinear sign-shift gate by the JCM, we construct the conditional sign-flip gate, which works with small error probability in principle. We also discuss two experimental setups for realizing our scheme. In the first experimental setup, we make use of coherent lights to examine whether or not our scheme works. In the second experimental setup, an optical loop circuit made out of the polarizing beam splitter and the Pockels cell takes an important role in the cavity.

  10. Oscillator-like coherent states for the Jaynes-Cummings Model

    NASA Technical Reports Server (NTRS)

    Berubelauziere, Y.; Hussin, V.; Nieto, Michael M.

    1995-01-01

    A new way of diagonalizing the Jaynes-Cummings Hamiltonian is proposed, which allows the definition of annihilation operators and coherent states for this model. Mean values and dispersions over these states are computed and interpreted.

  11. From the Jaynes-Cummings-Hubbard to the Dicke model

    NASA Astrophysics Data System (ADS)

    Schmidt, S.; Blatter, G.; Keeling, J.

    2013-11-01

    We discuss the Jaynes-Cummings-Hubbard model describing the superfluid-Mott insulator transition of polaritons (i.e., dressed photon-qubit states) in coupled qubit-cavity arrays in the crossover from strong to weak correlations. In the strongly correlated regime the phase diagram and the elementary excitations of lattice polaritons near the Mott lobes are calculated analytically using a slave-boson theory (SBT). The opposite regime of weakly interacting polariton superfluids is described by a weak-coupling mean-field theory for a generalized multi-mode Dicke model. We show that a remarkable relation between the two theories exists in the limit of large photon bandwidth and large negative detuning, i.e., when the nature of polariton quasiparticles becomes qubit-like. In this regime, the weak-coupling theory predicts the existence of a single Mott lobe with a change of the universality class of the phase transition at the tip of the lobe, in perfect agreement with the SBT. Moreover, the spectra of low energy excitations, i.e., the sound velocity of the Goldstone mode and the gap of the amplitude mode match exactly as calculated from both theories.

  12. Partial entangling power for the Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Xiong, Heng-Na; Lu, Xiao-Ming; Wang, Xiaoguang

    2012-01-01

    Partial entangling power provides the average amount of entanglement produced by a d1 × d2 bipartite unitary operator. The average is done over the initial distribution of the states of one of the subsystems. In this paper, we extend the expression of the partial entangling power to the case that d1 is finite and d2 is arbitrary. In particular, we give an explicit expression of partial entangling power for the 2 × ∞ system. The expression can be well applicable to the Jaynes-Cummings model (JCM). The results can recover the well-known phenomenon in the JCM. We explicitly discuss its behaviour in the large detuning case and at the resonance case. Comparing the two cases, we find that it is easier for the JCM in the large detuning case to reach and maintain its maximum entangling power, while for the JCM at resonance, the achievable maximum entangling power is larger. In addition, the time average partial entangling power is also discussed.

  13. Entanglement invariant for the double Jaynes-Cummings model

    SciTech Connect

    Sainz, Isabel; Bjoerk, Gunnar

    2007-10-15

    We study entanglement dynamics between four qubits interacting through two isolated Jaynes-Cummings Hamiltonians, via an entanglement measure based on the wedge product. We compare the results with similar results obtained using bipartite concurrence resulting in what is referred to as 'entanglement sudden death'. We find a natural entanglement invariant under evolution, demonstrating that entanglement spreads out over all of the system's degrees of freedom that become entangled through the interaction. We also provide an analysis of why certain initial states lose all their entanglement in a finite time, although their excitation and coherence vanish only asymptotically with time.

  14. Bistability effect in the extreme strong coupling regime of the Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Dombi, András; Vukics, András; Domokos, Peter

    2015-03-01

    We study the nonlinear response of a driven cavity QED system in the extreme strong coupling regime where the saturation photon number is below one by many orders of magnitude. In this regime, multi-photon resonances within the Jaynes-Cummings spectrum up to high order can be resolved. We identify an intensity and frequency range of the external coherent drive for which the system exhibits bistability instead of resonant multi-photon transitions. The cavity field evolves into a mixture of the vacuum and another quasi-classical state well separated in phase space. The corresponding time evolution of the outgoing intensity is a telegraph signal alternating between two attractors.

  15. Berry phase in a two-atom Jaynes-Cummings model with Kerr medium

    NASA Astrophysics Data System (ADS)

    Bu, Shen-Ping; Zhang, Guo-Feng; Liu, Jia; Chen, Zi-Yu

    2008-12-01

    The Jaynes-Cummings model (JCM) is an very important model for describing interaction between quantized electromagnetic fields and atoms in cavity quantum electrodynamics (QED). This model is generalized in many different directions since it predicts many novel quantum effects that can be verified by modern physics experimental technologies. In this paper, the Berry phase and entropy of the ground state for arbitrary photon number n of a two-atom Jaynes-Cummings model with Kerr-like medium are investigated. It is found that there is some correspondence between their images, especially the existence of a curve in the Δ-ɛ plane along which the energy, Berry phase and entropy all reach their special values. So it is available for detecting entanglement by applying Berry phase.

  16. Two-polariton bound states in the Jaynes-Cummings-Hubbard model

    SciTech Connect

    Wong, Max T. C.; Law, C. K.

    2011-05-15

    We examine the eigenstates of the one-dimensional Jaynes-Cummings-Hubbard model in the two-excitation subspace. We discover that two-excitation bound states emerge when the ratio of vacuum Rabi frequency to the tunneling rate between cavities exceeds a critical value. We determine the critical value as a function of the quasimomentum quantum number, and indicate that the bound states carry a strong correlation in which the two polaritons appear to be spatially confined together.

  17. Atom-field entanglement in the Jaynes-Cummings model without rotating wave approximation

    NASA Astrophysics Data System (ADS)

    Mirzaee, M.; Batavani, M.

    2015-04-01

    In this paper, we present a structure for obtaining the exact eigenfunctions and eigenvalues of the Jaynes-Cummings model (JCM) without the rotating wave approximation (RWA). We study the evolution of the system in the strong coupling region using the time evolution operator without RWA. The entanglement of the system without RWA is investigated using the Von Neumann entropy as an entanglement measure. It is interesting that in the weak coupling regime, the population of the atomic levels and Von Neumann entropy without RWA model shows a good agreement with the RWA whereas in strong coupling domain, the results of these two models are quite different.

  18. The f-deformed Jaynes-Cummings model and its nonlinear coherent states

    NASA Astrophysics Data System (ADS)

    de los Santos-Sánchez, O.; Récamier, J.

    2012-01-01

    Based on the f-oscillator formalism, we introduce a nonlinear Jaynes-Cummings model (NJCM) which is constructed from the standard JCM by deforming the single-mode field operators. Such a generalization of the JCM describes the interaction of a two-level atom with a single mode of the electromagnetic field in the presence of a nonlinear Kerr-like medium. Since the medium is modelled as an f-oscillator, it is possible to consider the field f-coherent states (nonlinear coherent states) and their evolution.

  19. Dynamics of Jaynes-Cummings Model in the Absence of Rotating-Wave Approximation

    NASA Astrophysics Data System (ADS)

    Fan, Yun-Xia; Liu, Tao; Feng, Mang; Wang, Ke-Lin

    2007-05-01

    The Jaynes-Cummings model (JCM) is studied in the absence of the rotating-wave approximation (RWA) by a coherent-state expansion technique. In comparison with the previous paper in which the coherent-state expansion was performed only to the third order, we carry out in this paper a complete expansion to demonstrate exactly the dynamics of the JCM without the RWA. Our study gives a systematic method to solve the non-RWA problem, which would be useful in various physical systems, e.g., in a system with an ultracold trapped ion experiencing the running waves of lasers.

  20. Two-Photon Jaynes-Cummings Model Governed by Milburn Equation with Phase Damping

    NASA Astrophysics Data System (ADS)

    Chen, Chang-Yong; Li, Shao-Hua; Liu, Zong-Liang

    2006-04-01

    In this paper, we find an analytic solution of the master equation of a non-resonant two-photon Jaynes-Cummings model (JCM) with phase damping with the help of the super-operator technique. We study the influence of phase damping on non-classical effects in the JCM, such as oscillations of the photon-number distribution, revivals of the atomic inversion, and sub-Possion photon statistics. It is demonstrated that the phase damping suppresses the revivals of the atomic inversion and non-classical effects of the cavity field in the JCM.

  1. Vacuum-induced Berry phases in single-mode Jaynes-Cummings models

    SciTech Connect

    Liu, Yu; Wei, L. F.; Jia, W. Z.; Liang, J. Q.

    2010-10-15

    Motivated by work [Phys. Rev. Lett. 89, 220404 (2002)] for detecting the vacuum-induced Berry phases with two-mode Jaynes-Cummings models (JCMs), we show here that, for a parameter-dependent single-mode JCM, certain atom-field states also acquired photon-number-dependent Berry phases after the parameter slowly changed and eventually returned to its initial value. This geometric effect related to the field quantization still exists, even if the field is kept in its vacuum state. Specifically, a feasible Ramsey interference experiment with a cavity quantum electrodynamics system is designed to detect the vacuum-induced Berry phase.

  2. A classical simulation of nonlinear Jaynes-Cummings and Rabi models in photonic lattices.

    PubMed

    Rodríguez-Lara, B M; Soto-Eguibar, Francisco; Cárdenas, Alejandro Zárate; Moya-Cessa, H M

    2013-05-20

    The interaction of a two-level atom with a single-mode quantized field is one of the simplest models in quantum optics. Under the rotating wave approximation, it is known as the Jaynes-Cummings model and without it as the Rabi model. Real-world realizations of the Jaynes-Cummings model include cavity, ion trap and circuit quantum electrodynamics. The Rabi model can be realized in circuit quantum electrodynamics. As soon as nonlinear couplings are introduced, feasible experimental realizations in quantum systems are drastically reduced. We propose a set of two photonic lattices that classically simulates the interaction of a single two-level system with a quantized field under field nonlinearities and nonlinear couplings as long as the quantum optics model conserves parity. We describe how to reconstruct the mean value of quantum optics measurements, such as photon number and atomic energy excitation, from the intensity and from the field, such as von Neumann entropy and fidelity, at the output of the photonic lattices. We discuss how typical initial states involving coherent or displaced Fock fields can be engineered from recently discussed Glauber-Fock lattices. As an example, the Buck-Sukumar model, where the coupling depends on the intensity of the field, is classically simulated for separable and entangled initial states. PMID:23736508

  3. Single-photon scattering in an optomechanical Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Ng, K. H.; Law, C. K.

    2016-04-01

    We investigate an optomechanical system which realizes the Jaynes-Cummings (JC) model known in cavity QED. Such a system consists of a single photon and an optomechanical cavity with two optical cavity modes and one mechanical mode. Under the resonance condition when the mechanical frequency is close to the frequency difference between the optical modes, the photon and phonons can be strongly coupled. We present an analytic solution of single-photon scattering and show that the spectrum of the scattered photon exhibits excitation-number-dependent Rabi splitting of the JC model. In addition, we examine the response of the mechanical mode to a sequence of single photons, with one photon in the cavity at a time. We show that sequential photon scattering can efficiently excite the mechanical mode and generate sub-Poisson phonon statistics.

  4. Vacuum-induced Berry phases in single-mode Jaynes-Cummings models

    NASA Astrophysics Data System (ADS)

    Liu, Yu; Wei, L. F.; Jia, W. Z.; Liang, J. Q.

    2010-10-01

    Motivated by work [Phys. Rev. Lett.PRLTAO0031-900710.1103/PhysRevLett.89.220404 89, 220404 (2002)] for detecting the vacuum-induced Berry phases with two-mode Jaynes-Cummings models (JCMs), we show here that, for a parameter-dependent single-mode JCM, certain atom-field states also acquired photon-number-dependent Berry phases after the parameter slowly changed and eventually returned to its initial value. This geometric effect related to the field quantization still exists, even if the field is kept in its vacuum state. Specifically, a feasible Ramsey interference experiment with a cavity quantum electrodynamics system is designed to detect the vacuum-induced Berry phase.

  5. Exact treatment of the Jaynes-Cummings model under the action of an external classical field

    NASA Astrophysics Data System (ADS)

    Abdalla, M. Sebawe; Khalil, E. M.; Obada, A. S.-F.

    2011-09-01

    We consider the usual Jaynes-Cummings model (JCM), in the presence of an external classical field. Under a certain canonical transformation for the Pauli operators, the system is transformed into the usual JCM. Using the equations of motion in the Heisenberg picture, exact solutions for the time-dependent dynamical operators are obtained. In order to calculate the expectation values of these operators, the wave function has been constructed. It has been shown that the classical field augments the atomic frequency ω0 and mixes the original atomic states. Changes of squeezing from one quadrature to another is also observed for a strong value of the coupling parameter of the classical field. Furthermore, the system in this case displays partial entanglement and the state of the field losses its purity.

  6. Photon spectra and statistics in generalized Jaynes-Cummings model with photon losses and atom motion

    NASA Astrophysics Data System (ADS)

    Gorokhov, A. V.; Sinaiski, I. E.

    2006-03-01

    The Jaynes-Cummings model (JCM) of two-level atom interacting with the photon mode in ideal cavity plays an essential role in modern quantum optics. In previous papers 1,2 an exact form of density matrix of the JCM with fixed atom position and photons dissipation was found. Here, taking into account the classical motion of the atom through the cavity, it is considered a case of nonideal cavity with zero temperature. We have obtained an exact expression for density matrix and calculated photon spectra and spectra of the mean number of photons in a cavity and and time dependencies of some values relevant for the one-atom maser theory.

  7. Equivalent spin-orbit interaction in the two-polariton Jaynes-Cummings-Hubbard model.

    PubMed

    Li, C; Zhang, X Z; Song, Z

    2015-01-01

    A cavity quantum electrodynamics (cavity-QED) system combines two or more distinct quantum components, exhibiting features not seen in the individual systems. In this work, we study the one-dimensional Jaynes-Cummings-Hubbard model in the two-excitation (two-polariton) subspace. We find that the centre momentum of two-excitation induces a magnetic flux piercing the equivalent Hamiltonian Hk in the invariant subspace with momentum k, which can be described as a 4-leg ladder in the auxiliary space. Furthermore, it is shown that the system in π-centre-momentum subspace is equivalent to a lattice system for spin-1 particle with spin-orbit coupling. On the basis of this concise description, a series of bound-pair eigenstates which display long-range polaritonic entanglement is presented as a simple application. PMID:26159665

  8. Linear entropies in the Jaynes Cummings model with intensity-dependent coupling in a phase-damped cavity

    NASA Astrophysics Data System (ADS)

    Zhou, Qing-Chun; Zhu, Shi-Ning

    2005-06-01

    We investigate the evolution of a quantum system described by the Jaynes-Cummings model with an arbitrary form of intensity-dependent coupling by displaying the linear entropies of the atom, field and atom-field system in the large detuning approximation. The cavity field is assumed to be coupled to a reservoir with a phase-damping coupling. The effects of cavity phase damping on the entanglement and coherence loss of such a system are studied.

  9. A note on approximate teleportation of an unknown atomic state in the two-photon Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    dSouza, A. D.; Cardoso, W. B.; Avelar, A. T.; Baseia, B.

    2009-04-01

    We consider recent schemes [J.M. Liu, B. Weng, Physica A 367 (2006) 215] to teleport unknown atomic states and superposition of zero- and two-photon states using the two-photon Jaynes-Cummings model. Here we do the same using the “full two-photon Jaynes-Cumming”, valid for arbitrary average number of photons. The success probability and fidelity of this teleportation are also considered.

  10. Trojan Wave Packets in the Quantum Cavity within the Extended Jaynes-Cummings Model

    NASA Astrophysics Data System (ADS)

    Kalinski, Matt

    2016-05-01

    Some time ago we have developed the theory of the Trojan Wave Packets (TWP) in the classical strong Circularly Polarized electromagnetic field in terms of the Mathieu generating functions. We have discovered that by the proper partitioning of the Coulomb spectrum i.e. by considering the deviation from the circularity and the vertical tilt of the undressed states as the new quantum numbers we can reduce the problem to the problem of several non-interacting quantum pendula for the Stark-Zeeman field dressed states. The TWP in the infinite physical space however turned out to be weakly unstable due to the spontaneous emission. Here we develop the theory in which the TWP is truly eternal when the electromagnetic interactions are considered quantum and the field is confined by the perfect quantum cavity boundary conditions. First we extend the Jaynes-Cummings (JC) model from the two to the infinite number of levels interacting with the one or two perfectly resonant quantum modes of the electromagnetic field. Similarly the model of JC and our previous pendular model the dressed electron-field eigenstates are constructed within the weakly interacting manifolds. Superpositions of those states are possible with the quantum electron density moving on the circular trajectories.

  11. Exact treatment of the Jaynes-Cummings model under the action of an external classical field

    SciTech Connect

    Abdalla, M. Sebawe; Khalil, E.M.; Obada, A.S.-F.

    2011-09-15

    We consider the usual Jaynes-Cummings model (JCM), in the presence of an external classical field. Under a certain canonical transformation for the Pauli operators, the system is transformed into the usual JCM. Using the equations of motion in the Heisenberg picture, exact solutions for the time-dependent dynamical operators are obtained. In order to calculate the expectation values of these operators, the wave function has been constructed. It has been shown that the classical field augments the atomic frequency {omega}{sub 0} and mixes the original atomic states. Changes of squeezing from one quadrature to another is also observed for a strong value of the coupling parameter of the classical field. Furthermore, the system in this case displays partial entanglement and the state of the field losses its purity. - Highlights: > The time-dependent JCM, in the presence of the classical field, is still one of the essential problems in the quantum optics. > A new approach is applied through a certain canonical transformation. > The classical field augments the atomic frequency {omega}{sub 0} and mixes the original atomic states.

  12. Thermal Effects in Jaynes-Cummings Model Derived with Low-Temperature Expansion

    NASA Astrophysics Data System (ADS)

    Azuma, Hiroo; Ban, Masashi

    In this paper, we investigate thermal effects of the Jaynes-Cummings model (JCM) at finite temperature with a perturbative approach. We assume a single two-level atom and a single cavity mode to be initially in the thermal equilibrium state and the thermal coherent state, respectively, at a certain finite low temperature. Describing this system with Thermo Field Dynamics formalism, we obtain a low-temperature expansion of the atomic population inversion in a systematic manner. Letting the system evolve in time with the JCM Hamiltonian, we examine thermal effects of the collapse and the revival of the Rabi oscillations by means of the third-order perturbation theory under the low-temperature limit, that is to say, using the low-temperature expansion up to the third-order terms. From an intuitive discussion, we can expect that the period of the revival of the Rabi oscillations becomes longer as the temperature rises. Numerical results obtained with the perturbation theory reproduce well this temperature dependence of the period.

  13. Quasiperiodicity in time evolution of the Bloch vector under the thermal Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Azuma, Hiroo; Ban, Masashi

    2014-07-01

    We study a quasiperiodic structure in the time evolution of the Bloch vector, whose dynamics is governed by the thermal Jaynes-Cummings model (JCM). Putting the two-level atom into a certain pure state and the cavity field into a mixed state in thermal equilibrium at initial time, we let the whole system evolve according to the JCM Hamiltonian. During this time evolution, motion of the Bloch vector seems to be in disorder. Because of the thermal photon distribution, both a norm and a direction of the Bloch vector change hard at random. In this paper, taking a different viewpoint compared with ones that we have been used to, we investigate quasiperiodicity of the Bloch vector’s trajectories. Introducing the concept of the quasiperiodic motion, we can explain the confused behaviour of the system as an intermediate state between periodic and chaotic motions. More specifically, we discuss the following two facts: (1) If we adjust the time interval Δt properly, figures consisting of plotted dots at the constant time interval acquire scale invariance under replacement of Δt by sΔt, where s(>1) is an arbitrary real but not transcendental number. (2) We can compute values of the time variable t, which let |Sz(t)| (the absolute value of the z-component of the Bloch vector) be very small, with the Diophantine approximation (a rational approximation of an irrational number).

  14. Exact mapping of the 2+1 Dirac oscillator onto the Jaynes-Cummings model: Ion-trap experimental proposal

    SciTech Connect

    Bermudez, A.; Martin-Delgado, M. A.; Solano, E.

    2007-10-15

    We study the dynamics of the 2+1 Dirac oscillator exactly and find spin oscillations due to a Zitterbewegung of purely relativistic origin. We find an exact mapping of this quantum-relativistic system onto a Jaynes-Cummings model, describing the interaction of a two-level atom with a quantized single-mode field. This equivalence allows us to map a series of quantum optical phenomena onto the relativistic oscillator and vice versa. We make a realistic experimental proposal, in reach with current technology, for studying the equivalence of both models using a single trapped ion.

  15. Quantum state collapse and revival under the anti-Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Lv, Dingshun; An, Shuoming; Um, Mark; Zhang, Junhua; Zhang, Jingning; Kim, M. S.; Kim, Kihwan; CenterQuantum Information, IIIS, Tsinghua University Team

    2015-05-01

    We study the evolution of a coherent state of phonon mode by anti-Jaynes-Cummings (AJC) interaction in a trapped 171Yb+ ion system. We observe the quantum collapse and revival phenomena by measuring its Q function at the several time intervals. We measure the Q-function by detecting the probability in the vacuum state through the conventional arithmetic subtraction. We also measure the corresponding Wigner function, and observe the negativity, which clearly shows non-classical state emergence during the AJC dynamic evolution. On top of the standard AJC evolution, we introduce an additional phase or Jaynes-Cummings (JC) coupling and control and reverse the dynamics. This work was supported by the National Basic Research Program of China under Grants No. 2011CBA00300 (No. 2011CBA00301), the National Natural Science Foundation of China 11374178. M.S. Kim was supported by the UK EPSRC and Royal Society Wolfson Merit Award.

  16. Fifty years of Jaynes-Cummings physics

    NASA Astrophysics Data System (ADS)

    Greentree, Andrew D.; Koch, Jens; Larson, Jonas

    2013-11-01

    This special issue commemorates the 50th anniversary of the seminal paper published by E T Jaynes and F W Cummings [1], the fundamental model which they introduced and now carries their names, and celebrates the remarkable host of exciting research on Jaynes-Cummings physics throughout the last five decades. The Jaynes-Cummings model has been taking the prominent stance as the 'hydrogen atom of quantum optics' [2]. Generally speaking, it provides a fundamental quantum description of the simplest form of coherent radiation-matter interaction. The Jaynes-Cummings model describes the interaction between a single electromagnetic mode confined to a cavity, and a two-level atom. Energy is exchanged between the field and the atom, which leads directly to coherent population oscillations (Rabi oscillations) and superposition states (dressed states). Being exactly solvable, the Jaynes-Cummings model serves as a most useful toy model, and as such it is a textbook example of the physicists' popular strategy of simplifying a complex problem to its most elementary constituents. Thanks to the simplicity of the Jaynes-Cummings model, this caricature of coherent light-matter interactions has never lost its appeal. The Jaynes-Cummings model is essential when discussing experiments in quantum electrodynamics (indeed the experimental motivation of the Jaynes-Cummings model was evident already in the original paper, dealing as it does with the development of the maser), and it has formed the starting point for much fruitful research ranging from ultra-cold atoms to cavity quantum electrodynamics. In fact, Jaynes-Cummings physics is at the very heart of the beautiful experiments by S Haroche and D Wineland, which recently earned them the 2012 Nobel Prize in physics. Indeed, as with most significant models in physics, the model is invoked in settings that go far beyond its initial framework. For example, recent investigations involving multi-level atoms, multiple atoms [3, 4], multiple

  17. Entanglement and the Jaynes-Cummings model with Rydberg-dressed atoms

    NASA Astrophysics Data System (ADS)

    Biedermann, Grant

    2016-05-01

    Controlling quantum entanglement between parts of a many-body system is the key to unlocking the power of quantum information processing for applications such as quantum computation, high-precision sensing, and simulation of many-body physics. Spin degrees of freedom of ultracold neutral atoms in their ground electronic state provide a natural platform given their long coherence times and our ability to control them with magneto-optical fields, but creating strong coherent coupling between spins has been challenging. We demonstrate for the first time a strong and tunable Rydberg-dressed interaction between spins of individually trapped cesium atoms with energy shifts of order 1 MHz in units of Planck's constant. We spectroscopically demonstrate that this system is isomorphic to a Jaynes-Cummings Hamiltonian, and observe the √{ N} nonlinearity of the Jaynes-Cummings ladder with a single symmetric Rydberg excitation. This interaction enables a ground-state spin-flip blockade, whereby simultaneous hyperfine spin flips of two atoms are blockaded due to their mutual interaction. We employ this spin-flip blockade to rapidly produce single-step Bell-state entanglement between atoms. This work was supported by the Laboratory Directed Research and Development program at Sandia National Laboratories and through the National Science Foundation's Center for Quantum Information and Control NSF-1212445.

  18. On the relationship between the classical Dicke-Jaynes-Cummings-Gaudin model and the nonlinear Schroedinger equation

    SciTech Connect

    Du, Dianlou; Geng, Xue

    2013-05-15

    In this paper, the relationship between the classical Dicke-Jaynes-Cummings-Gaudin (DJCG) model and the nonlinear Schroedinger (NLS) equation is studied. It is shown that the classical DJCG model is equivalent to a stationary NLS equation. Moreover, the standard NLS equation can be solved by the classical DJCG model and a suitably chosen higher order flow. Further, it is also shown that classical DJCG model can be transformed into the classical Gaudin spin model in an external magnetic field through a deformation of Lax matrix. Finally, the separated variables are constructed on the common level sets of Casimir functions and the generalized action-angle coordinates are introduced via the Hamilton-Jacobi equation.

  19. Exact time-dependent pointer state in a Jaynes-Cummings model with intensity-dependent level shift

    NASA Astrophysics Data System (ADS)

    Zhang, Xin; Xu, Chang; Ren, Zhongzhou

    2015-06-01

    We show that in the Jaynes-Cummings model with an intensity-dependent level shift whose magnitude is tuned to give rise to periodic collapse and revival, there exists a kind of time evolution where the two-level system and a coherent boson field stay absolutely disentangled throughout the evolution, in spite of the nonzero interaction between them. This constitutes an explicit example for an exact time-dependent pointer state of the two-level system, which perhaps distinguishes this evolution from all known disentangled evolutions in JC-related models. The construction of such an evolution is made possible by a vast level degeneracy in the energy level structure. Two possible ways to observe this phenomenon are also discussed. We believe that the present work could contribute to the understanding of pointer states.

  20. Equivalence of a compressible inviscid flow and the Bloch vector under the thermal Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Azuma, Hiroo; Ban, Masashi

    2015-07-01

    In this paper, we show that the time evolution of the Bloch vector governed by the thermal Jaynes-Cummings model is equivalent to a compressible inviscid flow with zero vorticity. Because of its quasiperiodicity, the dynamics of the Bloch vector includes countably infinite angular momenta as integrals of motion. Moreover, to derive the Bloch vector, we trace out the Hilbert space of the cavity field and remove entanglement between the single atom and the cavity mode. These facts indicate that the dynamics of the Bloch vector can be described with a hidden-variable model that has local determinism and a countably infinite number of degrees of freedom. Our results fit these considerations.

  1. Interaction of a Two-Level Atom with the Morse Potential in the Framework of Jaynes-Cummings Model

    NASA Astrophysics Data System (ADS)

    Setare R., M.; Sh., Barzanjeh

    2009-09-01

    A theoretical study of the dynamical behaviors of the interaction between a two-level atom with a Morse potential in the framework of the Jaynes-Cummings model (JCM) is discussed. We show that this system is equivalent to an intensity-dependent coupling between the two-level atom and the non-deformed single-mode radiation field in the presence of an additional nonlinear interaction. We study the dynamical properties of the system such as, atomic population inversion, the probability distribution of cavity-field, the Mandel parameter and atomic dipole squeezing. It is shown how the depth of the Morse potential can be affected by non-classical properties of the system. Moreover, the temporal evolution of the Husimi-distribution function is explored.

  2. Phase Distribution of the Output of Jaynes-Cummings Model with the Superposition of Squeezed Displaced Fock States

    NASA Astrophysics Data System (ADS)

    Abd Al-Kader, G. M.

    2006-05-01

    The Wigner quasi-probability function for the superposition of squeezed displaced Fock states (SDFS's) is reviewed. The interaction of these states with a two-level atom in cavity with the presence of additional Kerr medium is studied. Exact general matrix elements of the time-dependent operators of a Jaynes-Cummings model (JCM), in the presence of a Kerr medium, with these states are derived. We have obtained the phase distribution by two different ways: one is by Pegg-Barnett formalism, the second is by integration of the Wigner function over the radial variable. Results of these two approaches are compared. The Wigner phase distributions for some values of parameters are illustrated. The behaviors of the distributions have been shown as a function of the squeeze parameter in JCM.

  3. Fifty years of Jaynes-Cummings physics

    NASA Astrophysics Data System (ADS)

    Greentree, Andrew D.; Koch, Jens; Larson, Jonas

    2013-11-01

    This special issue commemorates the 50th anniversary of the seminal paper published by E T Jaynes and F W Cummings [1], the fundamental model which they introduced and now carries their names, and celebrates the remarkable host of exciting research on Jaynes-Cummings physics throughout the last five decades. The Jaynes-Cummings model has been taking the prominent stance as the 'hydrogen atom of quantum optics' [2]. Generally speaking, it provides a fundamental quantum description of the simplest form of coherent radiation-matter interaction. The Jaynes-Cummings model describes the interaction between a single electromagnetic mode confined to a cavity, and a two-level atom. Energy is exchanged between the field and the atom, which leads directly to coherent population oscillations (Rabi oscillations) and superposition states (dressed states). Being exactly solvable, the Jaynes-Cummings model serves as a most useful toy model, and as such it is a textbook example of the physicists' popular strategy of simplifying a complex problem to its most elementary constituents. Thanks to the simplicity of the Jaynes-Cummings model, this caricature of coherent light-matter interactions has never lost its appeal. The Jaynes-Cummings model is essential when discussing experiments in quantum electrodynamics (indeed the experimental motivation of the Jaynes-Cummings model was evident already in the original paper, dealing as it does with the development of the maser), and it has formed the starting point for much fruitful research ranging from ultra-cold atoms to cavity quantum electrodynamics. In fact, Jaynes-Cummings physics is at the very heart of the beautiful experiments by S Haroche and D Wineland, which recently earned them the 2012 Nobel Prize in physics. Indeed, as with most significant models in physics, the model is invoked in settings that go far beyond its initial framework. For example, recent investigations involving multi-level atoms, multiple atoms [3, 4], multiple

  4. Influence of the Kerr effect in a Mott insulator on the superfluid transition from the point of view of the Jaynes-Cummings-Hubbard model

    NASA Astrophysics Data System (ADS)

    Gomes, Clélio B. C.; Almeida, Francisco A. G.; Souza, Andre M. C.

    2016-04-01

    We have studied analytically the Jaynes-Cummings-Hubbard model for a one-dimensional optical lattice with the account of the Kerr-type nonlinearity under the fermionic approximation. We have found that an increase in the number of photons or in the detuning parameter favors the superfluid phase. We have also found that the nonlinear Kerr effect favors the Mott insulator phase, which is in agreement with experimental observations.

  5. A transparent mechanism of Q function splitting in a Jaynes-Cummings model with intensity-dependent level shift

    NASA Astrophysics Data System (ADS)

    Zhang, Xin; Xu, Chang; Ren, Zhongzhou

    2015-10-01

    We investigate the tripartite relationship between the collapse and revival, the Q function splitting and the energy level structure in the Jaynes-Cummings (JC) model with an intensity-dependent level shift whose magnitude is tuned to give rise to periodic collapse and revivals. We show that this constitutes a clearer demonstration of the mechanism of Q function splitting and its relation with the collapse and revival than the standard JC model itself. The eigenstates form two groups, both of which form equidistant ladders with differing energy intervals. This structure gives rise to the periodic splitting and reunion of the Q function. Only when the reunion happens, a non-vanishing mutual interference between the two groups is possible and gives rise to observable Rabi oscillations. The possibility of observing the phenomena using a Rubidium atom in a cavity is also discussed. We believe the present work could contribute to the understanding of the collapse and revival and the Q function-splitting phenomena.

  6. Application of Abel-Plana Formula for Collapse and Revival of Rabi Oscillations in Jaynes-Cummings Model

    NASA Astrophysics Data System (ADS)

    Azuma, Hiroo

    In this paper, we give an analytical treatment to study the behavior of the collapse and the revival of the Rabi oscillations in the Jaynes-Cummings model (JCM). The JCM is an exactly soluble quantum mechanical model, which describes the interaction between a two-level atom and a single cavity mode of the electromagnetic field. If we prepare the atom in the ground state and the cavity mode in a coherent state initially, the JCM causes the collapse and the revival of the Rabi oscillations many times in a complicated pattern in its time-evolution. In this phenomenon, the atomic population inversion is described with an intractable infinite series. (When the electromagnetic field is resonant with the atom, the nth term of this infinite series is given by a trigonometric function for √ {n} t, where t is a variable of the time.) According to Klimov and Chumakov's method, using the Abel-Plana formula, we rewrite this infinite series as a sum of two integrals. We examine the physical meanings of these two integrals and find that the first one represents the initial collapse (the semi-classical limit) and the second one represents the revival (the quantum correction) in the JCM. Furthermore, we evaluate the first- and second-order perturbations for the time-evolution of the JCM with an initial thermal coherent state for the cavity mode at low temperature, and write down their correction terms as sums of integrals by making use of the Abel-Plana formula.

  7. Entanglement, quantum phase transition and fixed-point bifurcation in the N-atom Jaynes Cummings model with an additional symmetry breaking term

    NASA Astrophysics Data System (ADS)

    Chagas, E. A.; Furuya, K.

    2008-08-01

    In the present work we analyze the quantum phase transition (QPT) in the N-atom Jaynes-Cummings model (NJCM) with an additional symmetry breaking interaction term in the Hamiltonian. We show that depending on the type of symmetry breaking term added the transition order can change or not and also the fixed point associated to the classical analogue of the Hamiltonian can bifurcate or not. We present two examples of symmetry broken Hamiltonians and discuss based on them, the interconnection between the transition order, appearance of bifurcation and the behavior of the entanglement.

  8. Atomic Dipole Squeezing in the Correlated Two-Mode Two-Photon Jaynes-Cummings Model

    NASA Technical Reports Server (NTRS)

    Dong, Zhengchao; Zhao, Yonglin

    1996-01-01

    In this paper, we study the atomic dipole squeezing in the correlated two-mode two-photon JC model with the field initially in the correlated two-mode SU(1,1) coherent state. The effects of detuning, field intensity and number difference between the two field modes are investigated through numerical calculation.

  9. ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS: Entropy and Entanglement in Master Equation of Effective Hamiltonian for Jaynes-Cummings Model

    NASA Astrophysics Data System (ADS)

    Hessian, H. A.; Mohammed, F. A.; Mohamed, A.-B. A.

    2009-04-01

    In this paper, we analytically solve the master equation for Jaynes-Cummings model in the dispersive regime including phase damping and the field is assumed to be initially in a superposition of coherent states. Using an established entanglement measure based on the negativity of the eigenvalues of the partially transposed density matrix we find a very strong sensitivity of the maximally generated entanglement to the amount of phase damping. Qualitatively this behavior is also reflected in alternative entanglement measures, but the quantitative agreement between different measures depends on the chosen noise model. The phase decoherence for this model results in monotonic increase in the total entropy while the atomic sub-entropy keeps its periodic behaviour without any effect.

  10. ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS: Properties of Linear Entropy in k-Photon Jaynes-Cummings Model with Stark Shift and Kerr-Like Medium

    NASA Astrophysics Data System (ADS)

    Liao, Qing-Hong; Ashfaq Ahmad, Muhammad; Wang, Yue-Yuan; Liu, Shu-Tian

    2010-05-01

    The time evolution of the linear entropy of an atom in k-photon Jaynes-Cummings model is investigated taking into consideration Stark shift and Kerr-like medium. The effect of both the Stark shift and Kerr-like medium on the linear entropy is analyzed using a numerical technique for the field initially in coherent state and in even coherent state. The results show that the presence of the Kerr-like medium and Stark shift has an important effect on the properties of the entropy and entanglement. It is also shown that the setting of the initial state plays a significant role in the evolution of the linear entropy and entanglement.

  11. Cavity losses for the dissipative Jaynes Cummings Hamiltonian beyond rotating wave approximation

    NASA Astrophysics Data System (ADS)

    Scala, M.; Militello, B.; Messina, A.; Maniscalco, S.; Piilo, J.; Suominen, K.-A.

    2007-11-01

    A microscopic derivation of the master equation for the Jaynes-Cummings model with cavity losses is given, taking into account the terms in the dissipator which vary with frequencies of the order of the vacuum Rabi frequency. Our approach allows us to single out physical contexts wherein the usual phenomenological dissipator turns out to be fully justified and constitutes an extension of our previous analysis (Scala et al 2007 Phys. Rev. A 75 013811), where a microscopic derivation was given in the framework of the rotating wave approximation.

  12. Thermal-light-induced dynamics: Coherence and revivals in V -type and molecular Jaynes-Cummings systems

    NASA Astrophysics Data System (ADS)

    Avisar, David; Wilson-Gordon, A. D.

    2016-03-01

    We examine the interaction of thermal light with matter with emphasis on two aspects that have not been considered before. By employing a fully quantized Jaynes-Cummings-type interaction model on a V -type three-level system, we show that multimode thermal light induces coherence in the excited material states. This is in contrast to previous studies that suggest thermal light cannot induce coherence in material systems. We also show that the ratio between the field detuning and the interaction constant has a significant influence on the characteristic time-dependent dynamics. In particular, for some ratio regimes, the thermal light induces dynamics with a "coherentlike" collapse and revivals pattern rather than the familiar pattern. We then extend the Jaynes-Cummings model to a two-state Born-Oppenheimer potential energy surface molecular system where the internal vibrational degrees of freedom are fully taken into account. The matter-field bipartite system is represented, and propagated, in the full electronic bond-coordinate Fock product space. We show that single-mode thermal light induces extensive excited-state vibrational coherence in the molecule that, when observed in coordinate space, exhibits wave-packet-like dynamics. The molecular Jaynes-Cummings model we propose is useful for cavity molecular dynamics simulations.

  13. Conditional nonlinear operations by sequential Jaynes-Cummings interactions

    NASA Astrophysics Data System (ADS)

    Park, Kimin; Marek, Petr; Filip, Radim

    2016-07-01

    Nonlinear operations are essential for quantum information processing. We propose a way of implementing a class of nonlinear operations by sequential application of conditional gates based on Jaynes-Cummings (JC) interaction and projective measurements. The scheme has many advantages over the previously proposed all-optical methods and can be applied in several available experimental platforms, such as cavity quantum electrodynamics, trapped ions, and others. We demonstrate performance of the approach on the example of the cubic nonlinearity. We show several different ways in which the full nonlinear operation can be decomposed into sequences of the individual gates, and we compare their performance.

  14. Reentrant Behavior in A Multi-connected Superconducting Jaynes-Cummings Lattice

    NASA Astrophysics Data System (ADS)

    Tian, Lin; Seo, Kangjun

    2015-03-01

    Superconducting quantum devices have excellent connectivity, tunable coupling and long decoherence time as demonstrated by recent experiments. These devices provide a powerful platform for constructing analog quantum simulators to study novel many-body effects. Here we present a multi-connected Jaynes-Cummings lattice model, where the qubits and the resonators are connected alternatively. In a one-dimensional configuration, this model bears an intrinsic symmetry between the left and the right qubit-resonator couplings under a mirror reflection. Different from the coupled cavity array (CCA) model, the qubit-resonator couplings in this model induce both onsite Hubbard nonlinearity and hopping of the excitations along the lattice. By analyzing this model in the limiting cases of very different couplings, we show that this model demonstrates a Mott insulator-superfluid-Mott insulator transition at commensurate fillings with symmetric critical points. The reentry to the Mott insulator phase originates from the symmetry between the couplings. This work is supported by the NSF Award 0956064.

  15. Quantum and classical chaos in kicked coupled Jaynes-Cummings cavities

    SciTech Connect

    Hayward, A. L. C.; Greentree, Andrew D.

    2010-06-15

    We consider two Jaynes-Cummings cavities coupled periodically with a photon hopping term. The semiclassical phase space is chaotic, with regions of stability over some ranges of the parameters. The quantum case exhibits dynamic localization and dynamic tunneling between classically forbidden regions. We explore the correspondence between the classical and quantum phase space and propose an implementation in a circuit QED system.

  16. Experimental investigation of a steady-state dynamical phase transition in a Jaynes-Cummings dimer

    NASA Astrophysics Data System (ADS)

    Raftery, James; Sadri, Darius; Mandt, Stephan; Tureci, Hakan; Houck, Andrew

    Experimental progress in circuit-QED has made it possible to study non-equilibrium many-body physics using strongly correlated photons. Such open and driven systems can display new types of dynamical phase transitions. A steady state transition has also been predicted for a Jaynes-Cummings dimer where the photon current between the two cavities acts as an order parameter. Here, we discuss the theory and report measurements of the steady-state behavior of a circuit-QED dimer with in situ tunable inter-cavity coupling and on-site photon-photon interaction. Recently deceased.

  17. Geometric quantum discord of a Jaynes-Cummings atom and an isolated atom

    NASA Astrophysics Data System (ADS)

    Qiang, Wen-Chao; Zhang, Lei; Zhang, Hua-Ping

    2015-12-01

    We studied the geometric quantum discord of a quantum system consisting of a Jaynes-Cummings (JC) atom, a cavity and an isolated atom. The analytical expressions of the geometric quantum discord for two atoms, every atom with a cavity and the total system were obtained. We showed that the geometric quantum discord is not always zero when the entanglement falls to zero for a two-atom subsystem; the geometric measurement of the quantum discord of the total system developed periodically with a single frequency if the initial two-atom state was not entangled, otherwise, it oscillated with two or four frequencies according to whether the cavity was initially empty or not, respectively.

  18. Entanglement dynamics of two independent Jaynes-Cummings atoms without the rotating-wave approximation

    SciTech Connect

    Chen Qinghu; Yang Yuan; Liu Tao; Wang Kelin

    2010-11-15

    Entanglement evolution of two independent Jaynes-Cummings atoms without the rotating-wave approximation (RWA) is studied by a numerically exact approach. Previous results based on the RWA are essentially modified in the strong-coupling regime (g{>=}0.1), which has been reached in the recent experiments on the flux qubit coupled to the LC resonator. For the initial Bell state with anticorrelated spins, entanglement sudden death (ESD) is absent in the RWA but does appear in the present numerical calculation without the RWA. Aperiodic entanglement evolution in the strong-coupling regime is observed. The strong atom-cavity coupling facilitates the ESD. The sign of the detuning plays an essential role in the entanglement evolution for strong coupling, which is irrelevant in the RWA. Analytical results based on an unitary transformation are also given, which could not modify the RWA picture essentially. It is suggested that the activation of the photons may be the origin of ESD in this system.

  19. Dynamics and improvement of quantum correlations in the triple Jaynes-Cummings model

    NASA Astrophysics Data System (ADS)

    Feng, Ling-Juan; Zhang, Ying-Jie; Xia, Yun-Jie

    2016-05-01

    We investigate the dynamics and improvement of tripartite quantum correlations in three atoms interacting with the independent cavities, in terms of genuinely multipartite concurrence, lower bound of concurrence and tripartite geometric quantum discord. By choosing the GHZ and W states as atomic initial states, we study the relationship between the initial state and entanglement transfer, and the robustness of different correlation measures. The results show that the different initial states can control entanglement transfer between the subsystems, and the tripartite geometric quantum discord is more robust than tripartite entanglement in the evolution process. Then, we propose the optimal scheme to improve tripartite entanglement in certain conditions via the weak measurement and quantum measurement reversal. In addition, we find that our study also works for the N-qubit GHZ state by using genuinely multipartite concurrence.

  20. Entanglement transfer from two-mode continuous variable SU(2) cat states to discrete qubits systems in Jaynes-Cummings Dimers

    PubMed Central

    Ran, Du; Hu, Chang-Sheng; Yang, Zhen-Biao

    2016-01-01

    We study the entanglement transfer from a two-mode continuous variable system (initially in the two-mode SU(2) cat states) to a couple of discrete two-state systems (initially in an arbitrary mixed state), by use of the resonant Jaynes-Cummings (JC) interaction. We first quantitatively connect the entanglement transfer to non-Gaussianity of the two-mode SU(2) cat states and find a positive correlation between them. We then investigate the behaviors of the entanglement transfer and find that it is dependent on the initial state of the discrete systems. We also find that the largest possible value of the transferred entanglement exhibits a variety of behaviors for different photon number as well as for the phase angle of the two-mode SU(2) cat states. We finally consider the influences of the noise on the transferred entanglement. PMID:27553881

  1. Entanglement transfer from two-mode continuous variable SU(2) cat states to discrete qubits systems in Jaynes-Cummings Dimers.

    PubMed

    Ran, Du; Hu, Chang-Sheng; Yang, Zhen-Biao

    2016-01-01

    We study the entanglement transfer from a two-mode continuous variable system (initially in the two-mode SU(2) cat states) to a couple of discrete two-state systems (initially in an arbitrary mixed state), by use of the resonant Jaynes-Cummings (JC) interaction. We first quantitatively connect the entanglement transfer to non-Gaussianity of the two-mode SU(2) cat states and find a positive correlation between them. We then investigate the behaviors of the entanglement transfer and find that it is dependent on the initial state of the discrete systems. We also find that the largest possible value of the transferred entanglement exhibits a variety of behaviors for different photon number as well as for the phase angle of the two-mode SU(2) cat states. We finally consider the influences of the noise on the transferred entanglement. PMID:27553881

  2. An Alternative Map from a 2 + 1 Dimensional Charged Dirac Oscillator in the Background of a Uniform Perpendicular Magnetic Field to a Quantum Optics Model

    NASA Astrophysics Data System (ADS)

    Hou, Yu-Long; Wang, Qing; Long, Zheng-Wen; Jing, Jian

    2015-05-01

    We propose an alternative map from the the 2-dimensional charged Dirac oscillator in the background of a uniform perpendicular magnetic field onto a quantum optics model which contains both Jaynes-Cummings (JC) and Anti-Jaynes-Cummings (AJC) interactions. Different from previous work, we only introduce one kind of phonons and realize a symmetrical competition which is controlled by the magnetic field. Furthermore, we find that this model behaves as a quantum phase transition when a dimensionless parameter crosses its critical value. Several characteristics of quantum phase transition are exhibited explicitly.

  3. Multiphoton-scattering theory and generalized master equations

    NASA Astrophysics Data System (ADS)

    Shi, Tao; Chang, Darrick E.; Cirac, J. Ignacio

    2015-11-01

    We develop a scattering theory to investigate the multiphoton transmission in a one-dimensional waveguide in the presence of quantum emitters. It is based on a path integral formalism, uses displacement transformations, and does not require the Markov approximation. We obtain the full time evolution of the global system, including the emitters and the photonic field. Our theory allows us to compute the transition amplitude between arbitrary initial and final states, as well as the S matrix of the asymptotic in and out states. For the case of few incident photons in the waveguide, we also rederive a generalized master equation in the Markov limit. We compare the predictions of the developed scattering theory and that with the Markov approximation. We illustrate our methods with five examples of few-photon scattering: (i) by a two-level emitter, (ii) in the Jaynes-Cummings model; (iii) by an array of two-level emitters; (iv) by a two-level emitter in the half-end waveguide; and (v) by an array of atoms coupled to Rydberg levels. In the first two, we show the application of the scattering theory in the photon scattering by a single emitter, and examine the correctness of our theory with the well-known results. In the third example, we analyze the condition of the Markov approximation for the photon scattering in the array of emitters. In the fourth one, we show how a quantum emitter can generate entanglement of outgoing photons. Finally, we highlight the interplay between the phenomenon of electromagnetic-induced transparency and the Rydberg interaction, and show how this results in a rich variety of possibilities in the quantum statistics of the scattering photons.

  4. Effects of the Stark Shift on the Evolution of the Field Entropy and Entanglement in the Two-Photon Jaynes-Cummings Model

    NASA Technical Reports Server (NTRS)

    Fang, Mao Fa

    1996-01-01

    The evolution of the field entropy in the two-photon JCM in the presence of the Stark shift is investigated, and the effects of the dynamic Stark shift on the evolution of the field entropy and entanglement between the atom and field, are examined. The results show that the dynamic Stark shift plays an important role in the evolution of the field entropy in two-photon processes.

  5. Exactly solvable models for atom-molecule Hamiltonians.

    PubMed

    Dukelsky, J; Dussel, G G; Esebbag, C; Pittel, S

    2004-07-30

    We present a family of exactly solvable generalizations of the Jaynes-Cummings model involving the interaction of an ensemble of SU(2) or SU(1,1) quasispins with a single boson field. They are obtained from the trigonometric Richardson-Gaudin models by replacing one of the SU(2) or SU(1,1) degrees of freedom by an ideal boson. The application to a system of bosonic atoms and molecules is reported. PMID:15323678

  6. General integrable n-level, many-mode Janes-Cummings-Dicke models and classical r-matrices with spectral parameters

    SciTech Connect

    Skrypnyk, T. E-mail: tskrypnyk@imath.kiev.ua

    2015-02-15

    Using the technique of classical r-matrices and quantum Lax operators, we construct the most general form of the quantum integrable “n-level, many-mode” spin-boson Jaynes-Cummings-Dicke-type hamiltonians describing an interaction of a molecule of N n-level atoms with many modes of electromagnetic field and containing, in general, additional non-linear interaction terms. We explicitly obtain the corresponding quantum Lax operators and spin-boson analogs of the generalized Gaudin hamiltonians and prove their quantum commutativity. We investigate symmetries of the obtained models that are associated with the geometric symmetries of the classical r-matrices and construct the corresponding algebra of quantum integrals. We consider in detail three classes of non-skew-symmetric classical r-matrices with spectral parameters and explicitly obtain the corresponding quantum Lax operators and Jaynes-Cummings-Dicke-type hamiltonians depending on the considered r-matrix.

  7. A simple model of multiphoton micromachining in silk hydrogels

    NASA Astrophysics Data System (ADS)

    Applegate, Matthew B.; Alonzo, Carlo; Georgakoudi, Irene; Kaplan, David L.; Omenetto, Fiorenzo G.

    2016-06-01

    High resolution three-dimensional voids can be directly written into transparent silk fibroin hydrogels using ultrashort pulses of near-infrared (NIR) light. Here, we propose a simple finite-element model that can be used to predict the size and shape of individual features under various exposure conditions. We compare predicted and measured feature volumes for a wide range of parameters and use the model to determine optimum conditions for maximum material removal. The simplicity of the model implies that the mechanism of multiphoton induced void creation in silk is due to direct absorption of light energy rather than diffusion of heat or other photoproducts, and confirms that multiphoton absorption of NIR light in silk is purely a 3-photon process.

  8. Analytical Solution for the Anisotropic Rabi Model: Effects of Counter-Rotating Terms

    PubMed Central

    Zhang, Guofeng; Zhu, Hanjie

    2015-01-01

    The anisotropic Rabi model, which was proposed recently, differs from the original Rabi model: the rotating and counter-rotating terms are governed by two different coupling constants. This feature allows us to vary the counter-rotating interaction independently and explore the effects of it on some quantum properties. In this paper, we eliminate the counter-rotating terms approximately and obtain the analytical energy spectrums and wavefunctions. These analytical results agree well with the numerical calculations in a wide range of the parameters including the ultrastrong coupling regime. In the weak counter-rotating coupling limit we find out that the counter-rotating terms can be considered as the shifts to the parameters of the Jaynes-Cummings model. This modification shows the validness of the rotating-wave approximation on the assumption of near-resonance and relatively weak coupling. Moreover, the analytical expressions of several physics quantities are also derived, and the results show the break-down of the U(1)-symmetry and the deviation from the Jaynes-Cummings model. PMID:25736827

  9. Eigenvalue-based determinants for scalar products and form factors in Richardson-Gaudin integrable models coupled to a bosonic mode

    NASA Astrophysics Data System (ADS)

    Claeys, Pieter W.; De Baerdemacker, Stijn; Van Raemdonck, Mario; Van Neck, Dimitri

    2015-10-01

    Starting from integrable su(2) (quasi-)spin Richardson-Gaudin (RG) XXZ models we derive several properties of integrable spin models coupled to a bosonic mode. We focus on the Dicke-Jaynes-Cummings-Gaudin models and the two-channel (p + ip)-wave pairing Hamiltonian. The pseudo-deformation of the underlying su(2) algebra is here introduced as a way to obtain these models in the contraction limit of different RG models. This allows for the construction of the full set of conserved charges, the Bethe ansatz state, and the resulting RG equations. For these models an alternative and simpler set of quadratic equations can be found in terms of the eigenvalues of the conserved charges. Furthermore, the recently proposed eigenvalue-based determinant expressions for the overlaps and form factors of local operators are extended to these models, linking the results previously presented for the Dicke-Jaynes-Cummings-Gaudin models with the general results for RG XXZ models.

  10. Demonstration of structural alterations in experimental corneal infectious model using multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Lo, Wen; Tan, Hsin-Yuan; Chang, Yuh-Ling; Sun, Yen; Lin, Sung-Jan; Jee, Shiou-Hwa; Dong, Chen-Yuan

    2007-02-01

    The aim of this study is to assess the application of multiphoton autofluorescence and second harmonic generation (SHG) microscopy for investigating the structural alterations and the pattern of microbial spreading during corneal infectious process in an in vitro organ culture model. The autofluorescence spectrum derived from pathogens allows us to monitoring the pattern of microbial spreading within corneal lamellae. In addition, the destruction and regeneration of second harmonic generating collagen during infectious process can also be monitored in a non-invasive fashion. Therefore we propose that multiphoton microscopy may potentially be applied as an effective monitoring tool for corneal infection studies.

  11. Multiphoton imaging of upconverting lanthanide nanoparticles in three dimensional models of cancer

    NASA Astrophysics Data System (ADS)

    Gainer, Christian F.; Romanowski, Marek

    2013-02-01

    While upconverting lanthanide nanoparticles have numerous advantages over other exogenous contrast agents used in scanned multiphoton imaging, their long luminescence lifetimes cause images collected with non-descanned detection to be greatly blurred. We demonstrate herein the use of Richardson-Lucy deconvolution to deblur luminescence images obtained via multiphoton scanning microscopy. Images were taken of three dimensional models of colon and ovarian cancer following incubation with NaYF4:Yb,Er nanoparticles functionalized with an antibody for EGFR and folic acid respectively. Following deconvolution, images had a lateral resolution on par with the optimal performance of the imaging system used, ~1.2 μm, and an axial resolution of ~5 μm. Due to the relatively high multiphoton excitation efficiency of these nanoparticles, it is possible to follow binding of individual particles in tissue. In addition, their extreme photostability allows for prolonged imaging without significant loss in luminescence signal. With these advantageous properties in mind, we also discuss the potential application of upconverting lanthanide nanoparticles for tracking of specific, cancer relevant receptors in tissue.

  12. Multiphoton Imaging of Upconverting Lanthanide Nanoparticles in Three Dimensional Models of Cancer

    PubMed Central

    Gainer, Christian F.; Romanowski, Marek

    2013-01-01

    While upconverting lanthanide nanoparticles have numerous advantages over other exogenous contrast agents used in scanned multiphoton imaging, their long luminescence lifetimes cause images collected with non-descanned detection to be greatly blurred. We demonstrate herein the use of Richardson-Lucy deconvolution to deblur luminescence images obtained via multiphoton scanning microscopy. Images were taken of three dimensional models of colon and ovarian cancer following incubation with NaYF4:Yb,Er nanoparticles functionalized with an antibody for EGFR and folic acid respectively. Following deconvolution, images had a lateral resolution on par with the optimal performance of the imaging system used, ~1.2 μm, and an axial resolution of ~5 μm. Due to the relatively high multiphoton excitation efficiency of these nanoparticles, it is possible to follow binding of individual particles in tissue. In addition, their extreme photostability allows for prolonged imaging without significant loss in luminescence signal. With these advantageous properties in mind, we also discuss the potential application of upconverting lanthanide nanoparticles for tracking of specific, cancer relevant receptors in tissue. PMID:24353385

  13. Quantum Rabi Model with Trapped Ions

    PubMed Central

    Pedernales, J. S.; Lizuain, I.; Felicetti, S.; Romero, G.; Lamata, L.; Solano, E.

    2015-01-01

    We propose the quantum simulation of the quantum Rabi model in all parameter regimes by means of detuned bichromatic sideband excitations of a single trapped ion. We show that current setups can reproduce, in particular, the ultrastrong and deep strong coupling regimes of such a paradigmatic light-matter interaction. Furthermore, associated with these extreme dipolar regimes, we study the controlled generation and detection of their entangled ground states by means of adiabatic methods. Ion traps have arguably performed the first quantum simulation of the Jaynes-Cummings model, a restricted regime of the quantum Rabi model where the rotating-wave approximation holds. We show that one can go beyond and experimentally investigate the quantum simulation of coupling regimes of the quantum Rabi model that are difficult to achieve with natural dipolar interactions. PMID:26482660

  14. Quantum Rabi Model with Trapped Ions.

    PubMed

    Pedernales, J S; Lizuain, I; Felicetti, S; Romero, G; Lamata, L; Solano, E

    2015-01-01

    We propose the quantum simulation of the quantum Rabi model in all parameter regimes by means of detuned bichromatic sideband excitations of a single trapped ion. We show that current setups can reproduce, in particular, the ultrastrong and deep strong coupling regimes of such a paradigmatic light-matter interaction. Furthermore, associated with these extreme dipolar regimes, we study the controlled generation and detection of their entangled ground states by means of adiabatic methods. Ion traps have arguably performed the first quantum simulation of the Jaynes-Cummings model, a restricted regime of the quantum Rabi model where the rotating-wave approximation holds. We show that one can go beyond and experimentally investigate the quantum simulation of coupling regimes of the quantum Rabi model that are difficult to achieve with natural dipolar interactions. PMID:26482660

  15. In vivo imaging of spinal cord in contusion injury model mice by multi-photon microscopy

    NASA Astrophysics Data System (ADS)

    Oshima, Y.; Horiuchi, H.; Ogata, T.; Hikita, A.; Miura, H.; Imamura, T.

    2014-03-01

    Fluorescent imaging technique is a promising method and has been developed for in vivo applications in cellular biology. In particular, nonlinear optical imaging technique, multi-photon microscopy has make it possible to analyze deep portion of tissues in living animals such as axons of spinal code. Traumatic spinal cord injuries (SCIs) are usually caused by contusion damages. Therefore, observation of spinal cord tissue after the contusion injury is necessary for understanding cellular dynamics in response to traumatic SCI and development of the treatment for traumatic SCI. Our goal is elucidation of mechanism for degeneration of axons after contusion injuries by establishing SCI model and chronic observation of injured axons in the living animals. Firstly we generated and observed acute SCI model by contusion injury. By using a multi-photon microscope, axons in dorsal cord were visualized approximately 140 micron in depth from the surface. Immediately after injury, minimal morphological change of spinal cord was observed. At 3 days after injury, spinal cord was swelling and the axons seem to be fragmented. At 7 days after injury, increased degradation of axons could be observed, although the image was blurred due to accumulation of the connective tissue. In the present study, we successfully observed axon degeneration after the contusion SCI in a living animal in vivo. Our final goal is to understand molecular mechanisms and cellular dynamics in response to traumatic SCIs in acute and chronic stage.

  16. MULTIPHOTON PROCESSES

    SciTech Connect

    2002-07-05

    The Gordon Research Conference (GRC) on MULTIPHOTON PROCESSES was held at Tilton School, Tilton, NH. Emphasis was placed on current unpublished research and discussion of the future target areas in this field.

  17. Use of multiphoton microscopy to diagnose liver cancer and lung metastasis in an orthotopic rat model.

    PubMed

    Yan, Jun; Zhuo, Shuangmu; Chen, Gang; Tan, Changjun; Zhu, Weifeng; Lu, Jianping; Fan, Jia; Chen, Jianxin; Zhou, Jian

    2012-01-01

    Liver or lung biopsy for suspicious lesions has several disadvantages such as bleeding, bile leak or pneumothorax, needle track seeding, and time-consuming histopathological procedure. The ability to directly observe cellular and subcellular details and then perform "optical biopsy" is a major goal in the development of new interventional techniques. Multiphoton microscopy (MPM) enables real-time noninvasive visualization of tissue architecture and cell morphology in live tissue. We performed a study to evaluate whether MPMcan make real-time optical diagnosis for liver cancer and lung metastasis using an orthotopic rat model with Morris hepatoma. We found that real-time high-resolution MPMimaging could clearly show tissue architecture and cell morphology. In the normal liver tissue, MPMimaging clearly revealed the blood-filled sinusoids and cords of hepatocytes. In the cancerous tissue, MPMimaging clearly illustrated that cancer cells displayed marked cellular and nuclear pleomorphism. MPMimages were comparable to golden standard hematoxylin-eosin staining images. Moreover, MPMimaging had deep penetration with the capability of optical sectioning. In short, MPMcan make real-time optical diagnosis for liver cancer and lung metastasis. This study provides the groundwork for further using multiphoton endoscopy to perform real-time noninvasive "optical biopsy" for liver cancer and lung metastasis in the near future. PMID:22331704

  18. Generalized Rabi models: Diagonalization in the spin subspace and differential operators of Dunkl type

    NASA Astrophysics Data System (ADS)

    Moroz, Alexander

    2016-03-01

    A discrete parity {Z}2 -symmetry of a two-parameter extension of the quantum Rabi model which smoothly interpolates between the latter and the Jaynes-Cummings model, and of the two-photon and the two-mode quantum Rabi models, enables their diagonalization in the spin subspace. A more general statement is that the respective sets of 2× 2 Hermitian operators of the Fulton-Gouterman type and those diagonal in the spin subspace are unitary equivalent. The diagonalized representation makes it transparent that any question about integrability and solvability can be addressed only at the level of ordinary differential operators of Dunkl type. Braak's definition of integrability is shown i) to contradict earlier numerical studies and ii) to imply that any physically reasonable differential operator of Fulton-Gouterman type is integrable.

  19. Modeling molecule-plasmon interactions using quantized radiation fields within time-dependent electronic structure theory

    SciTech Connect

    Nascimento, Daniel R.; DePrince, A. Eugene

    2015-12-07

    We present a combined cavity quantum electrodynamics/ab initio electronic structure approach for simulating plasmon-molecule interactions in the time domain. The simple Jaynes-Cummings-type model Hamiltonian typically utilized in such simulations is replaced with one in which the molecular component of the coupled system is treated in a fully ab initio way, resulting in a computationally efficient description of general plasmon-molecule interactions. Mutual polarization effects are easily incorporated within a standard ground-state Hartree-Fock computation, and time-dependent simulations carry the same formal computational scaling as real-time time-dependent Hartree-Fock theory. As a proof of principle, we apply this generalized method to the emergence of a Fano-like resonance in coupled molecule-plasmon systems; this feature is quite sensitive to the nanoparticle-molecule separation and the orientation of the molecule relative to the polarization of the external electric field.

  20. Modeling molecule-plasmon interactions using quantized radiation fields within time-dependent electronic structure theory.

    PubMed

    Nascimento, Daniel R; DePrince, A Eugene

    2015-12-01

    We present a combined cavity quantum electrodynamics/ab initio electronic structure approach for simulating plasmon-molecule interactions in the time domain. The simple Jaynes-Cummings-type model Hamiltonian typically utilized in such simulations is replaced with one in which the molecular component of the coupled system is treated in a fully ab initio way, resulting in a computationally efficient description of general plasmon-molecule interactions. Mutual polarization effects are easily incorporated within a standard ground-state Hartree-Fock computation, and time-dependent simulations carry the same formal computational scaling as real-time time-dependent Hartree-Fock theory. As a proof of principle, we apply this generalized method to the emergence of a Fano-like resonance in coupled molecule-plasmon systems; this feature is quite sensitive to the nanoparticle-molecule separation and the orientation of the molecule relative to the polarization of the external electric field. PMID:26646866

  1. A phasor approach analysis of multiphoton FLIM measurements of three-dimensional cell culture models

    NASA Astrophysics Data System (ADS)

    Lakner, P. H.; Möller, Y.; Olayioye, M. A.; Brucker, S. Y.; Schenke-Layland, K.; Monaghan, M. G.

    2016-03-01

    Fluorescence lifetime imaging microscopy (FLIM) is a useful approach to obtain information regarding the endogenous fluorophores present in biological samples. The concise evaluation of FLIM data requires the use of robust mathematical algorithms. In this study, we developed a user-friendly phasor approach for analyzing FLIM data and applied this method on three-dimensional (3D) Caco-2 models of polarized epithelial luminal cysts in a supporting extracellular matrix environment. These Caco-2 based models were treated with epidermal growth factor (EGF), to stimulate proliferation in order to determine if FLIM could detect such a change in cell behavior. Autofluorescence from nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) in luminal Caco-2 cysts was stimulated by 2-photon laser excitation. Using a phasor approach, the lifetimes of involved fluorophores and their contribution were calculated with fewer initial assumptions when compared to multiexponential decay fitting. The phasor approach simplified FLIM data analysis, making it an interesting tool for non-experts in numerical data analysis. We observed that an increased proliferation stimulated by EGF led to a significant shift in fluorescence lifetime and a significant alteration of the phasor data shape. Our data demonstrates that multiphoton FLIM analysis with the phasor approach is a suitable method for the non-invasive analysis of 3D in vitro cell culture models qualifying this method for monitoring basic cellular features and the effect of external factors.

  2. Multi-photon microscopy of tobacco-exposed organotypic skin models

    NASA Astrophysics Data System (ADS)

    Dao, Belinda; Yamazaki, Alissa; Sun, Chung Ho; Wang, Zifu; Pham, Nguyen; Oldham, Michael; Wong, Brian J. F.

    2006-02-01

    Cigarette smoking is the most preventable cause of death in the United States. Researchers have extensively studied smoking in regards to its association with cancer, cardiovascular, and pulmonary disease. In contrast, the impact of cigarette smoking on skin has received much less attention. To provide a better understanding of the effect of cigarette smoking on the human dermal layer, this study used multi-photon microscopy (MPM) to examine collagen in organotypic skin models exposed to cigarette smoke condensate (CSC). Adult and neonatal organotypic tissue-engineered artificial skin models (RAFTs) were constructed and exposed to varying concentrations of CSC. Imaging of the RAFTs was performed using MPM and second-harmonic generation signals (SHG), which allowed for collagen structure to be viewed and analyzed as well as for collagen density to be assessed from derived depth-dependent decay (DDD) values. RAFT contraction as related to exposure concentration was monitored as well. Results indicated a dose dependent between contraction rates and CSC concentration. Collagen structure showed more preservation of its original structure at a greater depth in RAFTs with higher concentrations of CSC. No clear trends could be drawn from analysis of derived DDD values.

  3. Structural analysis of articular cartilage using multiphoton microscopy: input for biomechanical modeling.

    PubMed

    Lilledahl, Magnus B; Pierce, David M; Ricken, Tim; Holzapfel, Gerhard A; Davies, Catharina de Lange

    2011-09-01

    The 3-D morphology of chicken articular cartilage was quantified using multiphoton microscopy (MPM) for use in continuum-mechanical modeling. To motivate this morphological study we propose aspects of a new, 3-D finite strain constitutive model for articular cartilage focusing on the essential load-bearing morphology: an inhomogeneous, poro-(visco)elastic solid matrix reinforced by an anisotropic, (visco)elastic dispersed fiber fabric which is saturated by an incompressible fluid residing in strain-dependent pores. Samples of fresh chicken cartilage were sectioned in three orthogonal planes and imaged using MPM, specifically imaging the collagen fibers using second harmonic generation. Employing image analysis techniques based on Fourier analysis, we derived the principal directionality and dispersion of the collagen fiber fabric in the superficial layer. In the middle layer, objective thresholding techniques were used to extract the volume fraction occupied by extracellular collagen matrix. In conjunction with information available in the literature, or additional experimental testing, we show how this data can be used to derive a 3-D map of the initial solid volume fraction and Darcy permeability. PMID:21478075

  4. Multiphoton microscopy for imaging infectious keratitis: demonstration of the pattern of microbial spread in an experimental model

    NASA Astrophysics Data System (ADS)

    Sun, Yen; Lo, Wen; Wu, Ruei-Jhih; Lin, Sung-Jan; Lin, Wei-Chou; Jee, Shiou-Hwa; Tan, Hsin-Yuan; Dong, Chen-Yuan

    2006-02-01

    The purpose of this study is to assess the application of multiphoton fluorescence and second harmonic generation (SHG) microscopy for imaging and monitoring the disease progress of infectious keratitis in an experimental model, and to investigate the possible correlation of tissue architecture with spreading patterns of pathogens in an experimental model. Porcine eyes are to be obtained from slaughter house and processed and placed in organ culture system. Fungal infections by common pathogens of infectious keratitis are to be induced in porcine cornea buttons. Multiphoton fluorescence and SHG microscopy will be used for imaging and for monitoring the progression and extension of tissue destruction and possibly the pattern of pathogen spreading. We found that SHG imaging is useful in identifying alterations to collagen architecture while autofluorescence microscopy can be used to visualize the fungi and cells within the stroma. In summary, multiphoton fluorescence and second harmonic generation microscopy can non-invasively demonstrate and monitor tissue destruction associated with infectious keratitis. The pattern of pathogen spreading and its correlation with the tissue architecture can also be shown, which can be useful for future studies of the tissue-microbial interactions for infectious keratitis.

  5. The Multiphoton Interaction of Lambda Model Atom and Two-Mode Fields

    NASA Technical Reports Server (NTRS)

    Liu, Tang-Kun

    1996-01-01

    The system of two-mode fields interacting with atom by means of multiphotons is addressed, and the non-classical statistic quality of two-mode fields with interaction is discussed. Through mathematical calculation, some new rules of non-classical effects of two-mode fields which evolue with time, are established.

  6. Modeling extracellular matrix (ECM) alterations in ovarian cancer by multiphoton excited fabrication of stromal models (Conference Presentation)

    NASA Astrophysics Data System (ADS)

    Campagnola, Paul J.; Ajeti, Visar; Lara, Jorge; Eliceiri, Kevin W.; Patankar, Mansh

    2016-04-01

    A profound remodeling of the extracellular matrix (ECM) occurs in human ovarian cancer but it unknown how this affects tumor growth, where this understanding could lead to better diagnostics and therapeutic approaches. We investigate the role of these ECM alterations by using multiphoton excited (MPE) polymerization to fabricate biomimetic models to investigate operative cell-matrix interactions in invasion/metastasis. First, we create nano/microstructured gradients mimicking the basal lamina to study adhesion/migration dynamics of ovarian cancer cells of differing metastatic potential. We find a strong haptotactic response that depends on both contact guidance and ECM binding cues. While we found enhanced migration for more invasive cells, the specifics of alignment and directed migration also depend on cell polarity. We further use MPE fabrication to create collagen scaffolds with complex, 3D submicron morphology. The stromal scaffold designs are derived directly from "blueprints" based on SHG images of normal, high risk, and malignant ovarian tissues. The models are seeded with different cancer cell lines and this allows decoupling of the roles of cell characteristics (metastatic potential) and ECM structure and composition (normal vs cancer) on adhesion/migration dynamics. We found the malignant stroma structure promotes enhanced migration and proliferation and also cytoskeletal alignment. Creating synthetic models based on fibers patterns further allows decoupling the topographic roles of the fibers themselves vs their alignment within the tissue. These models cannot be synthesized by other conventional fabrication methods and we suggest the MPE image-based fabrication method will enable a variety of studies in cancer biology.

  7. In vivo three-dimensional optical coherence tomography and multiphoton microscopy in a mouse model of ovarian neoplasia

    NASA Astrophysics Data System (ADS)

    Watson, Jennifer M.; Marion, Samuel L.; Rice, Photini Faith; Bentley, David L.; Besselsen, David; Utzinger, Urs; Hoyer, Patricia B.; Barton, Jennifer K.

    2013-03-01

    Our goal is to use optical coherence tomography (OCT) and multiphoton microscopy (MPM) to detect early tumor development in a mouse model of ovarian neoplasia. We hope to use information regarding early tumor development to create a diagnostic test for high-risk patients. In this study we collect in vivo images using OCT, second harmonic generation and two-photon excited fluorescence from non-vinylcyclohexene diepoxide (VCD)-dosed and VCD-dosed mice. VCD causes follicular apoptosis (simulating menopause) and leads to tumor development. Using OCT and MPM we visualized the ovarian microstructure and were able to see differences between non-VCD-dosed and VCD-dosed animals. This leads us to believe that OCT and MPM may be useful for detecting changes due to early tumor development.

  8. Multiphoton processes: conference proceedings

    SciTech Connect

    Lambropoulos, P.; Smith, S.J.

    1984-01-01

    The chapters of this volume represent the invited papers delivered at the conference. They are arranged according to thermatic proximity beginning with atoms and continuing with molecules and surfaces. Section headings include multiphoton processes in atoms, field fluctuations and collisions in multiphoton process, and multiphoton processes in molecules and surfaces. Abstracts of individual items from the conference were prepared separately for the data base. (GHT)

  9. In-vitro visualization of corneal wound healing in an organ culture model using multiphoton autofluorescence and second harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Lo, Wen; Chang, Yuh-Ling; Sun, Yen; Lin, Sung-Jan; Jee, Shiou-Hwa; Tan, Hsin-Yuan; Dong, Chen-Yuan

    2007-02-01

    The aim of this work is to image the wound healing process of cornea in an in vitro organ culture model with noninvasive multiphoton imaging modality. Autofluorescence and second harmonic generation (SHG) were respectively used to monitor the alterations of cellular and collagenous components during wound healing processes. Within additional developments, this approach may be applied to in vivo visualization of corneal structural destruction and the subsequent regeneration.

  10. Kinetic modeling of evolution of 3 + 1:Resonance enhanced multiphoton ionization plasma in argon at low pressures

    SciTech Connect

    Tholeti, Siva Sashank; Alexeenko, Alina A.; Shneider, Mikhail N.

    2014-06-15

    We present numerical kinetic modeling of generation and evolution of the plasma produced as a result of resonance enhanced multiphoton ionization (REMPI) in Argon gas. The particle-in-cell/Monte Carlo collision (PIC/MCC) simulations capture non-equilibrium effects in REMPI plasma expansion by considering the major collisional processes at the microscopic level: elastic scattering, electron impact ionization, ion charge exchange, and recombination and quenching for metastable excited atoms. The conditions in one-dimensional (1D) and two-dimensional (2D) formulations correspond to known experiments in Argon at a pressure of 5 Torr. The 1D PIC/MCC calculations are compared with the published results of local drift-diffusion model, obtained for the same conditions. It is shown that the PIC/MCC and diffusion-drift models are in qualitative and in reasonable quantitative agreement during the ambipolar expansion stage, whereas significant non-equilibrium exists during the first few 10 s of nanoseconds. 2D effects are important in the REMPI plasma expansion. The 2D PIC/MCC calculations produce significantly lower peak electron densities as compared to 1D and show a better agreement with experimentally measured microwave radiation scattering.

  11. MULTI-PHOTON PHOSPHOR FEASIBILITY RESEARCH

    SciTech Connect

    R. Graham; W. Chow

    2003-05-01

    Development of multi-photon phosphor materials for discharge lamps represents a goal that would achieve up to a doubling of discharge (fluorescent) lamp efficacy. This report reviews the existing literature on multi-photon phosphors, identifies obstacles in developing such phosphors, and recommends directions for future research to address these obstacles. To critically examine issues involved in developing a multi-photon phosphor, the project brought together a team of experts from universities, national laboratories, and an industrial lamp manufacturer. Results and findings are organized into three categories: (1) Multi-Photon Systems and Processes, (2) Chemistry and Materials Issues, and (3) Concepts and Models. Multi-Photon Systems and Processes: This category focuses on how to use our current understanding of multi-photon phosphor systems to design new phosphor systems for application in fluorescent lamps. The quickest way to develop multi-photon lamp phosphors lies in finding sensitizer ions for Gd{sup 3+} and identifying activator ions to red shift the blue emission from Pr{sup 3+} due to the {sup 1}S{sub 0} {yields} {sup 1}I{sub 6} transition associated with the first cascading step. Success in either of these developments would lead to more efficient fluorescent lamps. Chemistry and Materials Issues: The most promising multi-photon phosphors are found in fluoride hosts. However, stability of fluorides in environments typically found in fluorescent lamps needs to be greatly improved. Experimental investigation of fluorides in actual lamp environments needs to be undertaken while working on oxide and oxyfluoride alternative systems for backup. Concepts and Models: Successful design of a multi-photon phosphor system based on cascading transitions of Gd{sup 3+} and Pr{sup 3+} depends critically on how the former can be sensitized and the latter can sensitize an activator ion. Methods to predict energy level diagrams and Judd-Ofelt parameters of multi-photon

  12. Three-Dimensional Morphology by Multiphoton Microscopy with Clearing in a Model of Cisplatin-Induced CKD.

    PubMed

    Torres, Richard; Velazquez, Heino; Chang, John J; Levene, Michael J; Moeckel, Gilbert; Desir, Gary V; Safirstein, Robert

    2016-04-01

    Traditional histologic methods are limited in their ability to detect pathologic changes of CKD, of which cisplatin therapy is an important cause. In addition, poor reproducibility of available methods has limited analysis of the role of fibrosis in CKD. Highly labor-intensive serial sectioning studies have demonstrated that three-dimensional perspective can reveal useful morphologic information on cisplatin-induced CKD. By applying the new technique of multiphoton microscopy (MPM) with clearing to a new mouse model of cisplatin-induced CKD, we obtained detailed morphologic and collagen reconstructions of millimeter-thick renal sections that provided new insights into pathophysiology. Quantitative analysis revealed that a major long-term cisplatin effect is reduction in the number of cuboidal cells of the glomerular capsule, a change we term the "uncapped glomerulus lesion." Glomerulotubular disconnection was confirmed, but connection remnants between damaged tubules and atubular glomeruli were observed. Reductions in normal glomerular capsules corresponded to reductions in GFR. Mild increases in collagen were noted, but the fibrosis was not spatially correlated with atubular glomeruli. Glomerular volume and number remained unaltered with cisplatin exposure, but cortical tubulointerstitial mass decreased. In conclusion, new observations were made possible by using clearing MPM, demonstrating the utility of this technique for studies of renal disease. This technique should prove valuable for further characterizing the evolution of CKD with cisplatin therapy and of other conditions. PMID:26303068

  13. Examination of diagnostic features in multiphoton microscopy and optical coherence tomography images of ovarian tumorigenesis in a mouse model

    NASA Astrophysics Data System (ADS)

    Watson, Jennifer M.

    Ovarian cancer is a deadly disease owing to the non-specific symptoms and suspected rapid progression, leading to frequent late stage detection and poor prognosis. Medical imaging methods such as CT, MRI and ultrasound as well as serum testing for cancer markers have had extremely poor performance for early disease detection. Due to the poor performance of available screening methods, and the impracticality and ineffectiveness of taking tissue biopsies from the ovary, women at high risk for developing ovarian cancer are often advised to undergo prophylactic salpingo-oophorectomy. This surgery results in many side effects and is most often unnecessary since only a fraction of high risk women go on to develop ovarian cancer. Better understanding of the early development of ovarian cancer and characterization of morphological changes associated with early disease could lead to the development of an effective screening test for women at high risk. Optical imaging methods including optical coherence tomography (OCT) and multiphoton microscopy (MPM) are excellent tools for studying disease progression owing to the high resolution and depth sectioning capabilities. Further, these techniques are excellent for optical biopsy because they can image in situ non-destructively. In the studies described in this dissertation OCT and MPM are used to identify cellular and tissue morphological changes associated with early tumor development in a mouse model of ovarian cancer. This work is organized into three specific aims. The first aim is to use the images from the MPM phenomenon of second harmonic generation to quantitatively examine the morphological differences in collagen structure in normal mouse ovarian tissue and mouse ovarian tumors. The second aim is to examine the differences in endogenous two-photon excited fluorescence in normal mouse ovarian tissue and mouse ovarian tumors. The third and final aim is to identify changes in ovarian microstructure resulting from early

  14. Ground-state phase diagram of the quantum Rabi model

    NASA Astrophysics Data System (ADS)

    Ying, Zu-Jian; Liu, Maoxin; Luo, Hong-Gang; Lin, Hai-Qing; You, J. Q.

    2015-11-01

    The Rabi model plays a fundamental role in understanding light-matter interaction. It reduces to the Jaynes-Cummings model via the rotating-wave approximation, which is applicable only to the cases of near resonance and weak coupling. However, recent experimental breakthroughs in upgrading light-matter coupling order require understanding the physics of the full quantum Rabi model (QRM). Despite the fact that its integrability and energy spectra have been exactly obtained, the challenge to formulate an exact wave function in a general case still hinders physical exploration of the QRM. Here we unveil a ground-state phase diagram of the QRM, consisting of a quadpolaron and a bipolaron as well as their changeover in the weak-, strong-, and intermediate-coupling regimes, respectively. An unexpected overweighted antipolaron is revealed in the quadpolaron state, and a hidden scaling behavior relevant to symmetry breaking is found in the bipolaron state. An experimentally accessible parameter is proposed to test these states, which might provide novel insights into the nature of the light-matter interaction for all regimes of the coupling strengths.

  15. Multiphoton polymerization using optical trap assisted nanopatterning

    NASA Astrophysics Data System (ADS)

    Leitz, Karl-Heinz; Tsai, Yu-Cheng; Flad, Florian; Schäffer, Eike; Quentin, Ulf; Alexeev, Ilya; Fardel, Romain; Arnold, Craig B.; Schmidt, Michael

    2013-06-01

    In this letter, we show the combination of multiphoton polymerization and optical trap assisted nanopatterning (OTAN) for the additive manufacturing of structures with nanometer resolution. User-defined patterns of polymer nanostructures are deposited on a glass substrate by a 3.5 μm polystyrene sphere focusing IR femtosecond laser pulses, showing minimum feature sizes of λ/10. Feature size depends on the applied laser fluence and the bead surface spacing. A finite element model describes the intensity enhancement in the microbead focus. The results presented suggest that OTAN in combination with multiphoton processing is a viable technique for additive nanomanufacturing with sub-diffraction-limited resolution.

  16. Simultaneous optical coherence and multiphoton microscopy of skin-equivalent tissue models

    NASA Astrophysics Data System (ADS)

    Barton, Jennifer K.; Tang, Shuo; Lim, Ryan; Tromberg, Bruce J.

    2007-07-01

    Three-layer skin-equivalent models (rafts) were created consisting of a collagen/fibroblast layer and an air-exposed keratinocyte layer. Rafts were imaged with a tri-modality microscope including optical coherence (OC), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) channels. Some rafts were stained with Hoechst 33343 or rhodamine 123, and some were exposed to dimethyl sulfoxide (DMSO). OC microscopy revealed signal in cell cytoplasm and nuclear membranes, and a characteristic texture in the collagen/fibroblast layer. TPEF showed signal in cell cytoplasm and from collagen, and stained specimens revealed cell nuclei or mitochondria. There was little SHG in the keratinocyte layer, but strong signal from collagen bundles. Endogenous signals were severely attenuated in DMSO treated rafts; stained samples revealed shrunken and distorted cell structure. OC, TPEF, and SHG can provide complementary and non-destructive information about raft structure and effect of chemical agents.

  17. Quantum-optical model for the dynamics of high-order-harmonic generation

    NASA Astrophysics Data System (ADS)

    Gombkötő, Ákos; Czirják, Attila; Varró, Sándor; Földi, Péter

    2016-07-01

    We investigate a two-level atom in the field of a strong laser pulse. The resulting time-dependent polarization is the source of a radiation the frequency components of which are essentially harmonics of the driving field's carrier frequency. The time evolution of this secondary radiation is analyzed in terms of the expectation values of the photon-number operators for a large number of electromagnetic modes that are initially in the vacuum state. Our method is based on a multimode version of the Jaynes-Cummings-Paul model and can be generalized to different radiating systems as well. We show that, after the exciting pulse, the final distribution of the photon numbers is close to the conventional (Fourier-transform-based) power spectrum of the secondary radiation. The details of the high-order-harmonic spectra (HHG spectra) are also analyzed; for many-cycle excitations a clear physical interpretation is given in terms of the Floquet quasienergies. A first step towards the determination of the photon statistics of the high-order-harmonic modes reveals states with slightly super-Poissonian distribution.

  18. Dynamics of entanglement and 'attractor' states in the Tavis-Cummings model

    NASA Astrophysics Data System (ADS)

    Jarvis, C. E. A.; Rodrigues, D. A.; Györffy, B. L.; Spiller, T. P.; Short, A. J.; Annett, J. F.

    2009-10-01

    We study the time evolution of Nq two-level atoms (or qubits) interacting with a single mode of a quantized radiation field. In the case of two qubits, we show that for a set of initial conditions the reduced density matrix of the atomic system approaches that of a pure state at {\\textstyle\\frac{t_{r}}{4}} , halfway between that start of the collapse and the first mini-revival peak, where tr is the time of the main revival. The pure state approached is the same for a set of initial conditions and is thus termed an 'attractor state'. The set itself is termed the 'basin of attraction' and we concentrate on its features. Extending to more qubits, we find that attractors are a generic feature of the multiqubit Jaynes-Cummings model (JCM) and we therefore generalize the discovery by Gea-Banacloche for the one-qubit case. We give the 'basin of attraction' for Nq qubits and discuss the implications of the 'attractor' state in terms of the dynamics of Nq-body entanglement. We observe both the collapse and revival and the sudden birth/death of entanglement depending on the initial conditions.

  19. Multiphoton microscopy in neuroscience

    NASA Astrophysics Data System (ADS)

    Denk, Winfried

    2002-06-01

    The study of the nervous system requires to an exceptional extent observation of and experimentation on intact tissue. There, in particular, high-resolution optical microscopy benefits from the inherent advantages of multi-photon fluorescence excitation. Several cases will be presented from a number of different tissues and organisms, where multi-photon excited laser scanning fluorescence microscopy has been an essential experimental tool. Those examples include the discovery of biochemical coincidence detection in synaptic spines and the clarification of the underlying mechanism; the observation of sensory evoked dendritic signaling in intact animals and the observation of light induced calcium signals in the intact retina. Recently a fiber coupled two-photon microscopy has been developed that allows the imaging in moving animal.

  20. Clinical multiphoton FLIM tomography

    NASA Astrophysics Data System (ADS)

    König, Karsten

    2012-03-01

    This paper gives an overview on current clinical high resolution multiphoton fluorescence lifetime imaging in volunteers and patients. Fluorescence lifetime imaging (FLIM) in Life Sciences was introduced in Jena/Germany in 1988/89 based on a ZEISS confocal picosecond dye laser scanning microscope equipped with a single photon counting unit. The porphyrin distribution in living cells and living tumor-bearing mice was studied with high spatial, temporal, and spectral resolution. Ten years later, time-gated cameras were employed to detect dental caries in volunteers based on one-photon excitation of autofluorescent bacteria with long fluorescence lifetimes. Nowadays, one-photon FLIM based on picosecond VIS laser diodes are used to study ocular diseases in humans. Already one decade ago, first clinical twophoton FLIM images in humans were taken with the certified clinical multiphoton femtosecond laser tomograph DermaInspectTM. Multiphoton tomographs with FLIM modules are now operating in hospitals at Brisbane, Tokyo, Berlin, Paris, London, Modena and other European cities. Multiple FLIM detectors allow spectral FLIM with a temporal resolution down to 20 ps (MCP) / 250 ps (PMT) and a spectral resolution of 10 nm. Major FLIM applications include the detection of intradermal sunscreen and tattoo nanoparticles, the detection of different melanin types, the early diagnosis of dermatitis and malignant melanoma, as well as the measurement of therapeutic effects in pateints suffering from dermatitis. So far, more than 1,000 patients and volunteers have been investigated with the clinical multiphoton FLIM tomographs DermaInspectTM and MPTflexTM.

  1. Multiphoton Microwave Ionization of Rydberg Atoms

    NASA Astrophysics Data System (ADS)

    Gurian, Joshua Houston

    This thesis describes a series of multiphoton microwave experiments on Rydberg atoms when the microwave frequency is much greater than the classical Kepler frequency of the excited atoms. A new kHz pulse repetition frequency dye laser system was constructed for Rydberg lithium excitation with a linewidth as narrow as 3 GHz. This new laser system is used for first experiments of multiphoton microwave ionization of Rydberg lithium approaching the photoionization limit using 17 and 36 GHz microwave pulses. A multi-channel quantum defect model is presented that well describes the experimental results, indicating that these results are due to the coherent coupling of many atomic levels both above and below the classical ionization limit. Finally, preliminary results of measuring the final-state distributions of high lying Rydberg states after 17 GHz microwave pulses are presented.

  2. Calculation of multiphoton ionization processes

    NASA Technical Reports Server (NTRS)

    Chang, T. N.; Poe, R. T.

    1976-01-01

    We propose an accurate and efficient procedure in the calculation of multiphoton ionization processes. In addition to the calculational advantage, this procedure also enables us to study the relative contributions of the resonant and nonresonant intermediate states.

  3. Multiphoton high-resolution 3D imaging of Langerhans cells and keratinocytes in the mouse skin model adopted for epidermal powdered immunization.

    PubMed

    Mulholland, William J; Arbuthnott, Edward A H; Bellhouse, Brian J; Cornhill, J Frederick; Austyn, Jonathan M; Kendall, Mark A F; Cui, Zhanfeng; Tirlapur, Uday K

    2006-07-01

    Langerhans cells (LCs) can be targeted with DNA-coated gold micro-projectiles ("Gene Gun") to induce potent cellular and humoral immune responses. It is likely that the relative volumetric distribution of LCs and keratinocytes within the epidermis impacts on the efficacy of Gene Gun immunization protocols. This study quantified the three-dimensional (3D) distribution of LCs and keratinocytes in the mouse skin model with a near-infrared multiphoton laser-scanning microscope (NIR-MPLSM). Stratum corneum (SC) and viable epidermal thickness measured with MPLSM was found in close agreement with conventional histology. LCs were located in the vertical plane at a mean depth of 14.9 microm, less than 3 mum above the dermo-epidermal boundary and with a normal histogram distribution. This likely corresponds to the fact that LCs reside in the suprabasal layer (stratum germinativum). The nuclear volume of keratinocytes was found to be approximately 1.4 times larger than that of resident LCs (88.6 microm3). Importantly, the ratio of LCs to keratinocytes in mouse ear skin (1:15) is more than three times higher than that reported for human breast skin (1:53). Accordingly, cross-presentation may be more significant in clinical Gene Gun applications than in pre-clinical mouse studies. These interspecies differences should be considered in pre-clinical trials using mouse models. PMID:16645596

  4. Multiphoton ionization of Uracil

    NASA Astrophysics Data System (ADS)

    Prieto, Eladio; Martinez, Denhi; Guerrero, Alfonso; Alvarez, Ignacio; Cisneros, Carmen

    2016-05-01

    Multiphoton ionization and dissociation of Uracil using a Reflectron time of flight spectrometer was performed along with radiation from the second harmonic of a Nd:YAG laser. Uracil is one of the four nitrogen bases that belong to RNA. The last years special interest has been concentrated on the study of the effects under UV radiation in nucleic acids1 and also in the role that this molecule could have played in the origin and development of life on our planet.2 The MPI mass spectra show that the presence and intensity of the resulting ions strongly depend on the density power. The identification of the ions in the mass spectra is presented. The results are compared with those obtained in other laboratories under different experimental conditions and some of them show partial agreement.3 The present work was supported by CONACYT-Mexico Grant 165410 and DGAPA UNAM Grant IN101215 and IN102613.

  5. Video-rate resonant scanning multiphoton microscopy

    PubMed Central

    Kirkpatrick, Nathaniel D.; Chung, Euiheon; Cook, Daniel C.; Han, Xiaoxing; Gruionu, Gabriel; Liao, Shan; Munn, Lance L.; Padera, Timothy P.; Fukumura, Dai; Jain, Rakesh K.

    2013-01-01

    The abnormal tumor microenvironment fuels tumor progression, metastasis, immune suppression, and treatment resistance. Over last several decades, developments in and applications of intravital microscopy have provided unprecedented insights into the dynamics of the tumor microenvironment. In particular, intravital multiphoton microscopy has revealed the abnormal structure and function of tumor-associated blood and lymphatic vessels, the role of aberrant tumor matrix in drug delivery, invasion and metastasis of tumor cells, the dynamics of immune cell trafficking to and within tumors, and gene expression in tumors. However, traditional multiphoton microscopy suffers from inherently slow imaging rates—only a few frames per second, thus unable to capture more rapid events such as blood flow, lymphatic flow, and cell movement within vessels. Here, we report the development and implementation of a video-rate multiphoton microscope (VR-MPLSM) based on resonant galvanometer mirror scanning that is capable of recording at 30 frames per second and acquiring intravital multispectral images. We show that the design of the system can be readily implemented and is adaptable to various experimental models. As examples, we demonstrate the utility of the system to directly measure flow within tumors, capture metastatic cancer cells moving within the brain vasculature and cells in lymphatic vessels, and image acute responses to changes in a vascular network. VR-MPLSM thus has the potential to further advance intravital imaging and provide new insight into the biology of the tumor microenvironment. PMID:24353926

  6. Effect of multiphoton ionization on performance of crystalline lens.

    PubMed

    Gupta, Pradeep Kumar; Singh, Ram Kishor; Strickland, D; Campbell, M C W; Sharma, R P

    2014-12-15

    This Letter presents a model for propagation of a laser pulse in a human crystalline lens. The model contains a transverse beam diffraction effect, laser-induced optical breakdown for the creation of plasma via a multiphoton ionization process, and the gradient index (GRIN) structure. Plasma introduces the nonlinearity in the crystalline lens which affects the propagation of the beam. The multiphoton ionization process generates plasma that changes the refractive index and hence leads to the defocusing of the laser beam. The Letter also points out the relevance of the present investigation to cavitation bubble formation for restoring the elasticity of the eyes. PMID:25502994

  7. Multiphoton tomography of astronauts

    NASA Astrophysics Data System (ADS)

    König, Karsten; Weinigel, Martin; Pietruszka, Anna; Bückle, Rainer; Gerlach, Nicole; Heinrich, Ulrike

    2015-03-01

    Weightlessness may impair the astronaut's health conditions. Skin impairments belong to the most frequent health problems during space missions. Within the Skin B project, skin physiological changes during long duration space flights are currently investigated on three European astronauts that work for nearly half a year at the ISS. Measurements on the hydration, the transepidermal water loss, the surface structure, elasticity and the tissue density by ultrasound are conducted. Furthermore, high-resolution in vivo histology is performed by multiphoton tomography with 300 nm spatial and 200 ps temporal resolution. The mobile certified medical tomograph with a flexible 360° scan head attached to a mechano-optical arm is employed to measure two-photon autofluorescence and SHG in the volar forearm of the astronauts. Modification of the tissue architecture and of the fluorescent biomolecules NAD(P)H, keratin, melanin and elastin are detected as well as of SHG-active collagen. Thinning of the vital epidermis, a decrease of the autofluoresence intensity, an increase in the long fluorescence lifetime, and a reduced skin ageing index SAAID based on an increased collagen level in the upper dermis have been found. Current studies focus on recovery effects.

  8. Multiphoton Effects in Rutile.

    NASA Astrophysics Data System (ADS)

    Royce, Gerald A.

    Multiphoton effects are investigated in crystalline rutile TiO(,2) using Nd:YAG laser photons. The 1.06 micron laser is operated in Q-switched mode with intensities up to 1.4 x 10('6) watts/cm('2) on the rutile crystal. Photoconductivity measurements provide data indicating a mixture of modes for electrons to be photoionized. Assuming aluminum impurity as the contributing sites, the first order photionization cross section is found to be 1.5 x 10('-26) cm('2) and second order cross section is found to be 7.7 x 10('-51) cm('4)-s. No appreciable change in cross section is observed for circular versus linear polarization of the laser. Observations of the photo-emission of the laser illuminated crystal provide radiative relaxation times on the order of 100 nanoseconds with emission peaks at 4500 and 5000 angstroms plus a near infrared continuum out to 1 micron. The thermoluminescence of rutile shows a number of trapping levels between 0.4 and 0.8 eV below the conduction band. These are attributed to an aluminum impurity.

  9. Quantitative multiphoton imaging

    NASA Astrophysics Data System (ADS)

    König, Karsten; Weinigel, Martin; Breunig, Hans Georg; Uchugonova, Aisada

    2014-02-01

    Certified clinical multiphoton tomographs for label-free multidimensional high-resolution in vivo imaging have been introduced to the market several years ago. Novel tomographs include a flexible 360° scan head attached to a mechanooptical arm for autofluorescence and SHG imaging as well as a CARS module. Non-fluorescent lipids and water, mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen can be imaged in vivo with submicron resolution in human skin. Sensitive and rapid detectors allow single photon counting and the construction of 3D maps where the number of detected photons per voxel is depicted. Intratissue concentration profiles from endogenous as well exogenous substances can be generated when the number of detected photons can be correlated with the number of molecules with respect to binding and scattering behavior. Furthermore, the skin ageing index SAAID based on the ratio elastin/collagen as well as the epidermis depth based on the onset of SHG generation can be determined.

  10. Modeling of the initiation and evolution of a laser-ionized column in the lower atmosphere - 314.5 nm wavelength resonant multiphoton ionization of naturally occurring argon

    NASA Technical Reports Server (NTRS)

    Fetzer, G. J.; Stockley, J. E.

    1992-01-01

    A 3+1 resonant multiphoton ionization process in naturally occurring argon is studied at 314.5 nm as a candidate for providing a long ionized channel through the atmosphere. Results are presented which indicate peak electron densities up to 10 exp 8/cu cm can be created using laser intensities on the order of 10 exp 8 W/sq cm.

  11. In vivo multiphoton tomography and fluorescence lifetime imaging of human brain tumor tissue.

    PubMed

    Kantelhardt, Sven R; Kalasauskas, Darius; König, Karsten; Kim, Ella; Weinigel, Martin; Uchugonova, Aisada; Giese, Alf

    2016-05-01

    High resolution multiphoton tomography and fluorescence lifetime imaging differentiates glioma from adjacent brain in native tissue samples ex vivo. Presently, multiphoton tomography is applied in clinical dermatology and experimentally. We here present the first application of multiphoton and fluorescence lifetime imaging for in vivo imaging on humans during a neurosurgical procedure. We used a MPTflex™ Multiphoton Laser Tomograph (JenLab, Germany). We examined cultured glioma cells in an orthotopic mouse tumor model and native human tissue samples. Finally the multiphoton tomograph was applied to provide optical biopsies during resection of a clinical case of glioblastoma. All tissues imaged by multiphoton tomography were sampled and processed for conventional histopathology. The multiphoton tomograph allowed fluorescence intensity- and fluorescence lifetime imaging with submicron spatial resolution and 200 picosecond temporal resolution. Morphological fluorescence intensity imaging and fluorescence lifetime imaging of tumor-bearing mouse brains and native human tissue samples clearly differentiated tumor and adjacent brain tissue. Intraoperative imaging was found to be technically feasible. Intraoperative image quality was comparable to ex vivo examinations. To our knowledge we here present the first intraoperative application of high resolution multiphoton tomography and fluorescence lifetime imaging of human brain tumors in situ. It allowed in vivo identification and determination of cell density of tumor tissue on a cellular and subcellular level within seconds. The technology shows the potential of rapid intraoperative identification of native glioma tissue without need for tissue processing or staining. PMID:26830089

  12. High-intensity laser heating in liquids: Multiphoton absorption

    SciTech Connect

    Longtin, J.P.; Tien, C.L.

    1995-12-31

    At high laser intensities, otherwise transparent liquids can absorb strongly by the mechanism of multiphoton absorption, resulting in absorption and heating several orders of magnitude greater than classical, low-intensity mechanisms. The use of multiphoton absorption provides a new mechanism for strong, controlled energy deposition in liquids without bulk plasma formation, shock waves, liquid ejection, etc., which is of interest for many laser-liquid applications, including laser desorption of liquid films, laser particle removal, and laser water removal from microdevices. This work develops a microscopically based model of the heating during multiphoton absorption in liquids. The dependence on pulse duration, intensity, wavelength, repetition rate, and liquid properties is discussed. Pure water exposed to 266 nm laser radiation is investigated, and a novel heating mechanism for water is proposed that uses multiple-wavelength laser pulses.

  13. Multiphoton microspectroscopy of biological specimens

    NASA Astrophysics Data System (ADS)

    Lin, Bai-Ling; Kao, Fu-Jen; Cheng, Ping C.; Sun, Chi-Kuang; Chen, RangWu; Wang, YiMin; Chen, JianCheng; Wang, Yung-Shun; Liu, Tzu-Ming; Huang, Mao-Kuo

    2000-07-01

    The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro- spectroscopy of biological specimen at high spatial resolution. Chloroplasts in mesophyll protoplast of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.

  14. Advances in multiphoton microscopy technology

    PubMed Central

    Hoover, Erich E.; Squier, Jeff A.

    2013-01-01

    Multiphoton microscopy has enabled unprecedented dynamic exploration in living organisms. A significant challenge in biological research is the dynamic imaging of features deep within living organisms, which permits the real-time analysis of cellular structure and function. To make progress in our understanding of biological machinery, optical microscopes must be capable of rapid, targeted access deep within samples at high resolution. In this Review, we discuss the basic architecture of a multiphoton microscope capable of such analysis and summarize the state-of-the-art technologies for the quantitative imaging of biological phenomena. PMID:24307915

  15. Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

    PubMed Central

    Gatesman Ammer, Amanda; Hayes, Karen E.; Martin, Karen H.; Zhang, Lingqing; Spirou, George A.; Weed, Scott A.

    2011-01-01

    Loco-regional invasion of head and neck cancer is linked to metastatic risk and presents a difficult challenge in designing and implementing patient management strategies. Orthotopic mouse models of oral cancer have been developed to facilitate the study of factors that impact invasion and serve as model system for evaluating anti-tumor therapeutics. In these systems, visualization of disseminated tumor cells within oral cavity tissues has typically been conducted by either conventional histology or with in vivo bioluminescent methods. A primary drawback of these techniques is the inherent inability to accurately visualize and quantify early tumor cell invasion arising from the primary site in three dimensions. Here we describe a protocol that combines an established model for squamous cell carcinoma of the tongue (SCOT) with two-photon imaging to allow multi-vectorial visualization of lingual tumor spread. The OSC-19 head and neck tumor cell line was stably engineered to express the F-actin binding peptide LifeAct fused to the mCherry fluorescent protein (LifeAct-mCherry). Fox1nu/nu mice injected with these cells reliably form tumors that allow the tongue to be visualized by ex-vivo application of two-photon microscopy. This technique allows for the orthotopic visualization of the tumor mass and locally invading cells in excised tongues without disruption of the regional tumor microenvironment. In addition, this system allows for the quantification of tumor cell invasion by calculating distances that invaded cells move from the primary tumor site. Overall this procedure provides an enhanced model system for analyzing factors that contribute to SCOT invasion and therapeutic treatments tailored to prevent local invasion and distant metastatic spread. This method also has the potential to be ultimately combined with other imaging modalities in an in vivo setting. PMID:21808230

  16. Stochastic scanning multiphoton multifocal microscopy.

    PubMed

    Jureller, Justin E; Kim, Hee Y; Scherer, Norbert F

    2006-04-17

    Multiparticle tracking with scanning confocal and multiphoton fluorescence imaging is increasingly important for elucidating biological function, as in the transport of intracellular cargo-carrying vesicles. We demonstrate a simple rapid-sampling stochastic scanning multifocal multiphoton microscopy (SS-MMM) fluorescence imaging technique that enables multiparticle tracking without specialized hardware at rates 1,000 times greater than conventional single point raster scanning. Stochastic scanning of a diffractive optic generated 10x10 hexagonal array of foci with a white noise driven galvanometer yields a scan pattern that is random yet space-filling. SS-MMM creates a more uniformly sampled image with fewer spatio-temporal artifacts than obtained by conventional or multibeam raster scanning. SS-MMM is verified by simulation and experimentally demonstrated by tracking microsphere diffusion in solution. PMID:19516485

  17. Multiphoton microscopy of atheroslcerotic plaques

    NASA Astrophysics Data System (ADS)

    Lilledahl, Magnus B.; de Lange Davies, Catharina; Haugen, Olav A.; Svaasand, Lars O.

    2007-02-01

    Multiphoton microscopy is a techniques that fascilitates three dimensional imaging of intact, unstained tissue. Especially connective tissue has a relatively strong nonlinear optical response and can easily be imaged. Atherosclerosis is a disease where lipids accumulate in the vessel wall and there is a thickening of the intima by growth of a cap of connective tissue. The mechanical strength of this fibrous cap is of clinically importance. If the cap ruptures a thrombosis forms which can block a coronary vessel and therby causing myocardial infarction. Multiphoton microscopy can be used to image the fibrous cap and thereby determine the thickness of the cap and the structure of the connective fibres. This could possibly be developed into a diagnostic and clincal tool to monitor the vulnerability of a plaque and also to better understand the development of a plaque and effects of treatment. We have collected multiphoton microscopy images from atherosclerotic plaque in human aorta, both two photon excited fluorescens and second harmonic generated signal. The feasability of using this technique to determine the state of the plaque is explored.

  18. Nonlinear magic: multiphoton microscopy in the biosciences.

    PubMed

    Zipfel, Warren R; Williams, Rebecca M; Webb, Watt W

    2003-11-01

    Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the best noninvasive means of fluorescence microscopy in tissue explants and living animals. Coupled with transgenic mouse models of disease and 'smart' genetically encoded fluorescent indicators, its use is now increasing exponentially. Properly applied, it is capable of measuring calcium transients 500 microm deep in a mouse brain, or quantifying blood flow by imaging shadows of blood cells as they race through capillaries. With the multitude of possibilities afforded by variations of nonlinear optics and localized photochemistry, it is possible to image collagen fibrils directly within tissue through nonlinear scattering, or release caged compounds in sub-femtoliter volumes. PMID:14595365

  19. Multiphoton fluorescence microscopy of the live kidney in health and disease.

    PubMed

    Small, David M; Sanchez, Washington Y; Roy, Sandrine; Hickey, Michael J; Gobe, Glenda C

    2014-02-01

    The structural and functional heterogeneity of the kidney ensures a diversity of response in health and disease. Multiphoton microscopy has improved our understanding of kidney physiology and pathophysiology by enabling the visualization of the living kidney in comparison with the static view of previous technologies. The use of multiphoton microscopy with rodent models in conjunction with endogenous fluorescence and exogenous infused dyes permits the measurement of renal processes, such as glomerular permeability, juxtaglomerular apparatus function, tubulointerstitial function, tubulovascular interactions, vascular flow rate, and the intrarenal renin-angiotensin-aldosterone system. Subcellular processes, including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes apoptosis and necrosis, can also be measured by multiphoton microscopy. This has allowed valuable insight into the pathophysiology of diabetic nephropathy, renal ischemia-reperfusion injury, hypertensive nephropathy, as well as inflammatory responses of the kidney. The current review presents an overview of multiphoton microscopy with a focus on techniques for imaging the kidney and gives examples of instances where multiphoton microscopy has been utilized to study renal pathophysiology in the living kidney. With continued advancements in the field of biological optics and increased adoption in experimental nephrology, multiphoton microscopy will undoubtedly continue to create new paradigms in kidney disease. PMID:24525825

  20. Multiphoton cryo microscope with sample temperature control

    NASA Astrophysics Data System (ADS)

    Breunig, H. G.; Uchugonova, A.; König, K.

    2013-02-01

    We present a multiphoton microscope system which combines the advantages of multiphoton imaging with precise control of the sample temperature. The microscope provides online insight in temperature-induced changes and effects in plant tissue and animal cells with subcellular resolution during cooling and thawing processes. Image contrast is based on multiphoton fluorescence intensity or fluorescence lifetime in the range from liquid nitrogen temperature up to +600°C. In addition, micro spectra from the imaged regions can be recorded. We present measurement results from plant leaf samples as well as Chinese hamster ovary cells.

  1. Differential Multiphoton Laser Scanning Microscopy

    PubMed Central

    Field, Jeffrey J.; Sheetz, Kraig E.; Chandler, Eric V.; Hoover, Erich E.; Young, Michael D.; Ding, Shi-you; Sylvester, Anne W.; Kleinfeld, David; Squier, Jeff A.

    2016-01-01

    Multifocal multiphoton microscopy (MMM) in the biological and medical sciences has become an important tool for obtaining high resolution images at video rates. While current implementations of MMM achieve very high frame rates, they are limited in their applicability to essentially those biological samples that exhibit little or no scattering. In this paper, we report on a method for MMM in which imaging detection is not necessary (single element point detection is implemented), and is therefore fully compatible for use in imaging through scattering media. Further, we demonstrate that this method leads to a new type of MMM wherein it is possible to simultaneously obtain multiple images and view differences in excitation parameters in a single shot. PMID:27390511

  2. The multiphoton AC Stark effect

    NASA Astrophysics Data System (ADS)

    Rudolph, T. G.; Ficek, Z.; Freedhoff, H. S.

    1998-02-01

    We study the interaction of a two-level atom with two intense lasers: a strong laser of Rabi frequency 2Ω on resonance with the atomic transition, and a weaker laser detuned by 2Ω/n, i.e. by a subharmonic of the Rabi frequency of the first. The second laser "dresses" the dressed states created by the first in an n-photon process. We calculate the energy levels and eigenstates of this "doubly-dressed" atom, and find a new phenomenon: the splitting of the energy levels due to an n-photon coupling between them, resulting in a multiphoton AC Stark effect. We illustrate this effect in the fluorescence spectrum, and show that the spectrum contains triplets at the subharmonic as well as harmonic resonance frequencies with a clear dependence on the order n of the resonance and the ratio α of the Rabi frequencies of the lasers

  3. Multiphoton-Excited Serotonin Photochemistry

    PubMed Central

    Gostkowski, Michael L.; Allen, Richard; Plenert, Matthew L.; Okerberg, Eric; Gordon, Mary Jane; Shear, Jason B.

    2004-01-01

    We report photochemical and photophysical studies of a multiphoton-excited reaction of serotonin that previously has been shown to generate a photoproduct capable of emitting broadly in the visible spectral region. The current studies demonstrate that absorption of near-infrared light by an intermediate state prepared via three-photon absorption enhances the photoproduct formation yield, with the largest action cross sections (∼10−19 cm2) observed at the short-wavelength limit of the titanium:sapphire excitation source. The intermediate state is shown to persist for at least tens of nanoseconds and likely to be different from a previously reported oxygen-sensitive intermediate. In addition, the two-photon fluorescence action spectrum for the fluorescent photoproduct was determined and found to have a maximum at ∼780 nm (3.2 eV). A general mechanism for this photochemical process is proposed. PMID:15111435

  4. Multiphoton ionization of uranium hexafluoride

    NASA Astrophysics Data System (ADS)

    Armstrong, D. P.; Harkins, D. A.; Compton, R. N.; Ding, D.

    1994-01-01

    Multiphoton ionization (MPI) time-of-flight mass spectroscopy (TOFMS) and photoelectron spectroscopy (PES) studies of UF6 are reported using focused light from the Nd:YAG laser fundamental (λ=1064 nm) and its harmonics (λ=532, 355, or 266 nm), as well as other wavelengths provided by a tunable dye laser. The MPI mass spectra are dominated by the singly and multiply charged uranium ions rather than by the UF+x fragment ions, even at the lowest laser power densities at which signal could be detected. In general, the doubly charged uranium ion (U2+) intensity is much greater than that of the singly charged uranium ion (U+). For the case of the tunable dye laser experiments, the Un+ (n=1-4) wavelength dependence is relatively unstructured and does not show observable resonance enhancement at known atomic uranium excitation wavelengths. The MPI-PES studies reveal only very slow electrons (≤0.5 eV) for all wavelengths investigated. The dominance of the U2+ ion, the absence or very small intensities of UF+x (x=1-3) fragments, the unstructured wavelength dependence, and the preponderance of slow electrons all indicate that mechanisms may exist other than ionization of bare U atoms following the stepwise photodissociation of F atoms from the parent molecule. The data also argue against stepwise photodissociation of UF+x (x=5,6) ions. Neither of the traditional MPI mechanisms (``neutral ladder'' or the ``ionic ladder'') are believed to adequately describe the ionization phenomena observed. We propose that the multiphoton excitation of UF6 under these experimental conditions results in a highly excited molecule, superexcited UF6**. The excitation of highly excited UF6** is proposed to be facilitated by the well known ``giant resonance,'' whose energy level lies in the range of 12-14 eV above that of ground state UF6. The highly excited molecule then primarily dissociates, via multiple channels, into Un+, UF+x, fluorine atoms, and ``slow'' electrons, although dissociation

  5. Resummation for Nonequilibrium Perturbation Theory and Application to Open Quantum Lattices

    NASA Astrophysics Data System (ADS)

    Li, Andy C. Y.; Petruccione, F.; Koch, Jens

    2016-04-01

    Lattice models of fermions, bosons, and spins have long served to elucidate the essential physics of quantum phase transitions in a variety of systems. Generalizing such models to incorporate driving and dissipation has opened new vistas to investigate nonequilibrium phenomena and dissipative phase transitions in interacting many-body systems. We present a framework for the treatment of such open quantum lattices based on a resummation scheme for the Lindblad perturbation series. Employing a convenient diagrammatic representation, we utilize this method to obtain relevant observables for the open Jaynes-Cummings lattice, a model of special interest for open-system quantum simulation. We demonstrate that the resummation framework allows us to reliably predict observables for both finite and infinite Jaynes-Cummings lattices with different lattice geometries. The resummation of the Lindblad perturbation series can thus serve as a valuable tool in validating open quantum simulators, such as circuit-QED lattices, currently being investigated experimentally.

  6. Clinical multiphoton and CARS microscopy

    NASA Astrophysics Data System (ADS)

    Breunig, H. G.; Weinigel, M.; Darvin, M. E.; Lademann, J.; König, K.

    2012-03-01

    We report on clinical CARS imaging of human skin in vivo with the certified hybrid multiphoton tomograph CARSDermaInspect. The CARS-DermaInspect provides simultaneous imaging of non-fluorescent intradermal lipid and water as well as imaging of two-photon excited fluorescence from intrinsic molecules. Two different excitation schemes for CARS imaging have been realized: In the first setup, a combination of fs oscillator and optical parametric oscillator provided fs-CARS pump and Stokes pulses, respectively. In the second setup a fs oscillator was combined with a photonic crystal fiber which provided a broadband spectrum. A spectral range out of the broadband-spectrum was selected and used for CARS excitation in combination with the residual fs-oscillator output. In both setups, in addition to CARS, single-beam excitation was used for imaging of two-photon excited fluorescence and second harmonic generation signals. Both CARS-excitation systems were successfully used for imaging of lipids inside the skin in vivo.

  7. Multi-photon excitation microscopy

    PubMed Central

    Diaspro, Alberto; Bianchini, Paolo; Vicidomini, Giuseppe; Faretta, Mario; Ramoino, Paola; Usai, Cesare

    2006-01-01

    Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter. In conjunction with confocal microscopy it can be considered the imaging workhorse of life science laboratories. Its roots can be found in a fundamental work written by Maria Goeppert Mayer more than 70 years ago. Nowadays, 2PE and MPE microscopes are expected to increase their impact in areas such biotechnology, neurobiology, embryology, tissue engineering, materials science where imaging can be coupled to the possibility of using the microscopes in an active way, too. As well, 2PE implementations in noninvasive optical bioscopy or laser-based treatments point out to the relevance in clinical applications. Here we report about some basic aspects related to the phenomenon, implications in three-dimensional imaging microscopy, practical aspects related to design and realization of MPE microscopes, and we only give a list of potential applications and variations on the theme in order to offer a starting point for advancing new applications and developments. PMID:16756664

  8. Multi-photon excitation microscopy.

    PubMed

    Diaspro, Alberto; Bianchini, Paolo; Vicidomini, Giuseppe; Faretta, Mario; Ramoino, Paola; Usai, Cesare

    2006-01-01

    Multi-photon excitation (MPE) microscopy plays a growing role among microscopical techniques utilized for studying biological matter. In conjunction with confocal microscopy it can be considered the imaging workhorse of life science laboratories. Its roots can be found in a fundamental work written by Maria Goeppert Mayer more than 70 years ago. Nowadays, 2PE and MPE microscopes are expected to increase their impact in areas such biotechnology, neurobiology, embryology, tissue engineering, materials science where imaging can be coupled to the possibility of using the microscopes in an active way, too. As well, 2PE implementations in noninvasive optical bioscopy or laser-based treatments point out to the relevance in clinical applications. Here we report about some basic aspects related to the phenomenon, implications in three-dimensional imaging microscopy, practical aspects related to design and realization of MPE microscopes, and we only give a list of potential applications and variations on the theme in order to offer a starting point for advancing new applications and developments. PMID:16756664

  9. Structure of multiphoton quantum optics. I. Canonical formalism and homodyne squeezed states

    SciTech Connect

    Dell'Anno, Fabio; De Siena, Silvio; Illuminati, Fabrizio

    2004-03-01

    We introduce a formalism of nonlinear canonical transformations for general systems of multiphoton quantum optics. For single-mode systems the transformations depend on a tunable free parameter, the homodyne local-oscillator angle; for n-mode systems they depend on n heterodyne mixing angles. The canonical formalism realizes nontrivial mixing of pairs of conjugate quadratures of the electromagnetic field in terms of homodyne variables for single-mode systems, and in terms of heterodyne variables for multimode systems. In the first instance the transformations yield nonquadratic model Hamiltonians of degenerate multiphoton processes and define a class of non-Gaussian, nonclassical multiphoton states that exhibit properties of coherence and squeezing. We show that such homodyne multiphoton squeezed states are generated by unitary operators with a nonlinear time evolution that realizes the homodyne mixing of a pair of conjugate quadratures. Tuning of the local-oscillator angle allows us to vary at will the statistical properties of such states. We discuss the relevance of the formalism for the study of degenerate (up-)down-conversion processes. In a companion paper [F. Dell'Anno, S. De Siena, and F. Illuminati, 69, 033813 (2004)], we provide the extension of the nonlinear canonical formalism to multimode systems, we introduce the associated heterodyne multiphoton squeezed states, and we discuss their possible experimental realization.

  10. Structure of multiphoton quantum optics. I. Canonical formalism and homodyne squeezed states

    NASA Astrophysics Data System (ADS)

    dell'Anno, Fabio; de Siena, Silvio; Illuminati, Fabrizio

    2004-03-01

    We introduce a formalism of nonlinear canonical transformations for general systems of multiphoton quantum optics. For single-mode systems the transformations depend on a tunable free parameter, the homodyne local-oscillator angle; for n -mode systems they depend on n heterodyne mixing angles. The canonical formalism realizes nontrivial mixing of pairs of conjugate quadratures of the electromagnetic field in terms of homodyne variables for single-mode systems, and in terms of heterodyne variables for multimode systems. In the first instance the transformations yield nonquadratic model Hamiltonians of degenerate multiphoton processes and define a class of non-Gaussian, nonclassical multiphoton states that exhibit properties of coherence and squeezing. We show that such homodyne multiphoton squeezed states are generated by unitary operators with a nonlinear time evolution that realizes the homodyne mixing of a pair of conjugate quadratures. Tuning of the local-oscillator angle allows us to vary at will the statistical properties of such states. We discuss the relevance of the formalism for the study of degenerate (up-)down-conversion processes. In a companion paper [

    F. Dell’Anno, S. De Siena, and F. Illuminati, 69, 033813 (2004)
    ], we provide the extension of the nonlinear canonical formalism to multimode systems, we introduce the associated heterodyne multiphoton squeezed states, and we discuss their possible experimental realization.

  11. Zeno physics in ultrastrong-coupling circuit QED

    SciTech Connect

    Lizuain, I.; Casanova, J.; Muga, J. G.; Garcia-Ripoll, J. J.; Solano, E.

    2010-06-15

    We study the Zeno and anti-Zeno effects in a superconducting qubit interacting strongly and ultrastrongly with a microwave resonator. Using a model of a frequently measured two-level system interacting with a quantized mode, we predict different behaviors and total control of the Zeno times depending on whether the rotating-wave approximation can be applied in the Jaynes-Cummings model. As an example, we show the dependence of our results with the properties of the initial field states.

  12. Polarization phenomena in multiphoton ionization of atoms

    NASA Technical Reports Server (NTRS)

    Jacobs, V. L.

    1973-01-01

    The theory of multiphoton ionization for an atomic system of arbitrary complexity is developed using a density matrix formalism. An expression is obtained which determines the differential N-photon ionization cross section as a function of the polarization states of the target atom and the incident radiation. The parameters which characterize the photoelectron angular distribution are related to the general reduced matrix elements for the N-photon transition. Two-photon ionization of unpolarized atoms is treated as an illustration of the use of the theory. The dependence of the multiphoton ionization cross section on the polarization state of the incident radiation, which has been observed in two- and three-photon ionization of Cs, is accounted for by the theory. Finally, the photoelectron spin polarization produced by the multiphoton ionization of unpolarized atoms, like the analogous polarization resulting from single-photon ionization, is found to depend on the circular polarization of the incident radiation.

  13. A pragmatic guide to multiphoton microscope design

    PubMed Central

    Young, Michael D.; Field, Jeffrey J.; Sheetz, Kraig E.; Bartels, Randy A.; Squier, Jeff

    2016-01-01

    Multiphoton microscopy has emerged as a ubiquitous tool for studying microscopic structure and function across a broad range of disciplines. As such, the intent of this paper is to present a comprehensive resource for the construction and performance evaluation of a multiphoton microscope that will be understandable to the broad range of scientific fields that presently exploit, or wish to begin exploiting, this powerful technology. With this in mind, we have developed a guide to aid in the design of a multiphoton microscope. We discuss source selection, optical management of dispersion, image-relay systems with scan optics, objective-lens selection, single-element light-collection theory, photon-counting detection, image rendering, and finally, an illustrated guide for building an example microscope. PMID:27182429

  14. Multiphoton absorption in amyloid protein fibres

    NASA Astrophysics Data System (ADS)

    Hanczyc, Piotr; Samoc, Marek; Norden, Bengt

    2013-12-01

    Fibrillization of peptides leads to the formation of amyloid fibres, which, when in large aggregates, are responsible for diseases such as Alzheimer's and Parkinson's. Here, we show that amyloids have strong nonlinear optical absorption, which is not present in native non-fibrillized protein. Z-scan and pump-probe experiments indicate that insulin and lysozyme β-amyloids, as well as α-synuclein fibres, exhibit either two-photon, three-photon or higher multiphoton absorption processes, depending on the wavelength of light. We propose that the enhanced multiphoton absorption is due to a cooperative mechanism involving through-space dipolar coupling between excited states of aromatic amino acids densely packed in the fibrous structures. This finding will provide the opportunity to develop nonlinear optical techniques to detect and study amyloid structures and also suggests that new protein-based materials with sizable multiphoton absorption could be designed for specific applications in nanotechnology, photonics and optoelectronics.

  15. Multiphoton microscopy in defining liver function

    NASA Astrophysics Data System (ADS)

    Thorling, Camilla A.; Crawford, Darrell; Burczynski, Frank J.; Liu, Xin; Liau, Ian; Roberts, Michael S.

    2014-09-01

    Multiphoton microscopy is the preferred method when in vivo deep-tissue imaging is required. This review presents the application of multiphoton microscopy in defining liver function. In particular, multiphoton microscopy is useful in imaging intracellular events, such as mitochondrial depolarization and cellular metabolism in terms of NAD(P)H changes with fluorescence lifetime imaging microscopy. The morphology of hepatocytes can be visualized without exogenously administered fluorescent dyes by utilizing their autofluorescence and second harmonic generation signal of collagen, which is useful in diagnosing liver disease. More specific imaging, such as studying drug transport in normal and diseased livers are achievable, but require exogenously administered fluorescent dyes. If these techniques can be translated into clinical use to assess liver function, it would greatly improve early diagnosis of organ viability, fibrosis, and cancer.

  16. New developments in multimodal clinical multiphoton tomography

    NASA Astrophysics Data System (ADS)

    König, Karsten

    2011-03-01

    80 years ago, the PhD student Maria Goeppert predicted in her thesis in Goettingen, Germany, two-photon effects. It took 30 years to prove her theory, and another three decades to realize the first two-photon microscope. With the beginning of this millennium, first clinical multiphoton tomographs started operation in research institutions, hospitals, and in the cosmetic industry. The multiphoton tomograph MPTflexTM with its miniaturized flexible scan head became the Prism-Award 2010 winner in the category Life Sciences. Multiphoton tomographs with its superior submicron spatial resolution can be upgraded to 5D imaging tools by adding spectral time-correlated single photon counting units. Furthermore, multimodal hybrid tomographs provide chemical fingerprinting and fast wide-field imaging. The world's first clinical CARS studies have been performed with a hybrid multimodal multiphoton tomograph in spring 2010. In particular, nonfluorescent lipids and water as well as mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen have been imaged in patients with dermatological disorders. Further multimodal approaches include the combination of multiphoton tomographs with low-resolution imaging tools such as ultrasound, optoacoustic, OCT, and dermoscopy systems. Multiphoton tomographs are currently employed in Australia, Japan, the US, and in several European countries for early diagnosis of skin cancer (malignant melanoma), optimization of treatment strategies (wound healing, dermatitis), and cosmetic research including long-term biosafety tests of ZnO sunscreen nanoparticles and the measurement of the stimulated biosynthesis of collagen by anti-ageing products.

  17. Multiphoton coherent control in complex systems

    PubMed Central

    Goswami, Debabrata

    2005-01-01

    Control of multiphoton transitions is demonstrated for a multilevel system by generalizing the instantaneous phase of any chirped pulse as individual terms of a Taylor series expansion. In the case of a simple two-level system, all odd terms in the series lead to population inversion while the even terms lead to self-induced transparency. The results hold for multiphoton transitions that do not have any lower-order photon resonance or any intermediate virtual state dynamics within the laser pulse width. PMID:17396157

  18. The collapse and revival of Bell-nonlocality of two macroscopic fields interacting with resonant atoms

    NASA Astrophysics Data System (ADS)

    Luo, Cheng-Li; Liao, Chang-Geng; Chen, Zi-Hong

    2010-08-01

    We investigate the nonlocality dynamics of two initially entangled macroscopic fields each interacting with a resonant two-level atom. The nonlocality of macroscopic field is characterized by the extent to which the Bell Clauser-Horne-Shimony-Holt (CHSH)'s inequality for continuous-variable states is violated. We show that the collapse and revival of the Bell-nonlocality are similar to the collapse and revival of the atomic population inversion of the Jaynes-Cummings model (JCM).

  19. One-atom maser theory: photon losses and spectra

    NASA Astrophysics Data System (ADS)

    Gorokhov, A. V.; Sinaiski, I. E.

    2005-06-01

    The Jaynes-Cummings model (JCM) of two-level atom interacting with the photon mode in ideal cavity plays an essential role in modern quantum optics. In previous papers an exact form of density matrix of the JCM with photons dissipation was found. In this article it is considered a partial case of cavity with zero temperature and obtained an exact expression for photon spectra and spectra of the mean number of photons in a cavity.

  20. Cavity quantum electrodynamics with a Rydberg-blocked atomic ensemble

    SciTech Connect

    Guerlin, Christine; Brion, Etienne; Esslinger, Tilman; Moelmer, Klaus

    2010-11-15

    The realization of a Jaynes-Cummings model in the optical domain is proposed for an atomic ensemble. The scheme exploits the collective coupling of the atoms to a quantized cavity mode and the nonlinearity introduced by coupling to high-lying Rydberg states. A two-photon transition resonantly couples the single-atom ground state |g> to a Rydberg state |e> via a nonresonant intermediate state |i>, but due to the interaction between Rydberg atoms only a single atom can be resonantly excited in the ensemble. This restricts the state space of the ensemble to the collective ground state |G> and the collectively excited state |E> with a single Rydberg excitation distributed evenly on all atoms. The collectively enhanced coupling of all atoms to the cavity field with coherent coupling strengths which are much larger than the decay rates in the system leads to the strong coupling regime of the resulting effective Jaynes-Cummings model. We use numerical simulations to show that the cavity transmission can be used to reveal detailed properties of the Jaynes-Cummings ladder of excited states and that the atomic nonlinearity gives rise to highly nontrivial photon emission from the cavity. Finally, we suggest that the absence of interactions between remote Rydberg atoms may, due to a combinatorial effect, induce a cavity-assisted excitation blockade whose range is larger than the typical Rydberg dipole-dipole interaction length.

  1. Multiphoton microscopy as a diagnostic imaging modality for lung cancer

    NASA Astrophysics Data System (ADS)

    Pavlova, Ina; Hume, Kelly R.; Yazinski, Stephanie A.; Peters, Rachel M.; Weiss, Robert S.; Webb, Watt W.

    2010-02-01

    Lung cancer is the leading killer among all cancers for both men and women in the US, and is associated with one of the lowest 5-year survival rates. Current diagnostic techniques, such as histopathological assessment of tissue obtained by computed tomography guided biopsies, have limited accuracy, especially for small lesions. Early diagnosis of lung cancer can be improved by introducing a real-time, optical guidance method based on the in vivo application of multiphoton microscopy (MPM). In particular, we hypothesize that MPM imaging of living lung tissue based on twophoton excited intrinsic fluorescence and second harmonic generation can provide sufficient morphologic and spectroscopic information to distinguish between normal and diseased lung tissue. Here, we used an experimental approach based on MPM with multichannel fluorescence detection for initial discovery that MPM spectral imaging could differentiate between normal and neoplastic lung in ex vivo samples from a murine model of lung cancer. Current results indicate that MPM imaging can directly distinguish normal and neoplastic lung tissues based on their distinct morphologies and fluorescence emission properties in non-processed lung tissue. Moreover, we found initial indication that MPM imaging differentiates between normal alveolar tissue, inflammatory foci, and lung neoplasms. Our long-term goal is to apply results from ex vivo lung specimens to aid in the development of multiphoton endoscopy for in vivo imaging of lung abnormalities in various animal models, and ultimately for the diagnosis of human lung cancer.

  2. Multiphoton Microscopy of Nonfluorescent Nanoparticles In Vitro and In Vivo.

    PubMed

    Dietzel, Steffen; Hermann, Stefanie; Kugel, Yan; Sellner, Sabine; Uhl, Bernd; Hirn, Stephanie; Krombach, Fritz; Rehberg, Markus

    2016-06-01

    Nanotechnology holds great promise for a plethora of potential applications. The interaction of engineered nanomaterials with living cells, tissues, and organisms is, however, only partly understood. Microscopic investigations of nano-bio interactions are mostly performed with a few model nanoparticles (NPs) which are easy to visualize, such as fluorescent quantum dots. Here the possibility to visualize nonfluorescent NPs with multiphoton excitation is investigated. Signals from silver (Ag), titanium dioxide (TiO2 ), and silica (SiO2 ) NPs in nonbiological environments are characterized to determine signal dependency on excitation wavelength and intensity as well as their signal stability over time. Ag NPs generate plasmon-induced luminescence decaying over time. TiO2 NPs induce photoluminescent signals of variable intensities and in addition strong third harmonic generation (THG). Optimal settings for microscopic detection are determined and then applied for visualization of these two particle types in living cells, in murine muscle tissue, and in the murine blood stream. Silica NPs produce a THG signal, but in living cells it cannot be discriminated sufficiently from endogenous cellular structures. It is concluded that multiphoton excitation is a viable option for studies of nano-bio interactions not only for fluorescent but also for some types of nonfluorescent NPs. PMID:27120195

  3. Formalism for multiphoton plasmon excitation in jellium clusters

    NASA Astrophysics Data System (ADS)

    Connerade, Jean-Patrick; Solov'yov, Andrey V.

    2002-07-01

    We present a formalism for the description of multiphoton plasmon excitation processes in jellium clusters. By using our method, we demonstrate that, in addition to dipole plasmon excitations, the multipole plasmons (quadrupole, octupole, etc.) can be excited in a cluster by multiphoton absorption processes, which results in a significant difference between plasmon resonance profiles in the cross sections for multiphoton as compared to single-photon absorption. We calculate the cross sections for multiphoton absorption and analyze the balance between the surface and volume plasmon contributions to multipole plasmons.

  4. In vivo multiphoton tomography of skin cancer

    NASA Astrophysics Data System (ADS)

    König, Karsten; Riemann, Iris; Ehlers, Alexander; Buckle, Rainer; Dimitrow, Enrico; Kaatz, Martin; Fluhr, Joachim; Elsner, Peter

    2006-02-01

    The multiphoton tomograph DermaInspect was used to perform first clinical studies on the early non-invasive detection of skin cancer based on non-invasive optical sectioning of skin by two-photon autofluorescence and second harmonic generation. In particular, deep-tissue pigmented lesions -nevi- have been imaged with intracellular resolution using near infrared (NIR) femtosecond laser radiation. So far, more than 250 patients have been investigated. Cancerous tissues showed significant morphological differences compared to normal skin layers. In the case of malignant melanoma, the occurrence of luminescent melanocytes has been detected. Multiphoton tomography will become a novel non-invasive method to obtain high-resolution 3D optical biopsies for early cancer detection, treatment control, and in situ drug screening.

  5. Multiphoton tomography of intratissue tattoo nanoparticles

    NASA Astrophysics Data System (ADS)

    König, Karsten

    2012-02-01

    Most of today's intratissue tattoo pigments are unknown nanoparticles. So far, there was no real control of their use due to the absence of regulations. Some of the tattoo pigments contain carcinogenic amines e.g. azo pigment Red 22. Nowadays, the European Union starts to control the administration of tattoo pigments. There is an interest to obtain information on the intratissue distribution, their interaction with living cells and the extracellular matrix, and the mechanisms behind laser tattoo removal. Multiphoton tomographs are novel biosafety and imaging tools that can provide such information non-invasively and without further labeling. When using the spectral FLIM module, spatially-resolved emission spectra, excitation spectra, and fluorescence lifetimes can pr provided. Multiphoton tomographs are used by all major cosmetic comapanies to test the biosafety of sunscreen nanoparticles.

  6. Multiphoton Microscopy for Visualizing Lipids in Tissue.

    PubMed

    Lee, Martin; Serrels, Alan

    2016-01-01

    Visualizing the appearance of fat droplets and adipocytes in tissue can be realized using a label-free imaging method known as coherent anti-Stokes Raman spectroscopy (CARS). CARS is a nonlinear optical technique that allows label-free imaging of a material with contrast based on the same vibrational signatures of molecules found in Raman spectroscopy. CARS can be combined with other single and multiphoton imaging modes such as second harmonic generation and two-photon fluorescence to image a broad variety of biological structures.Here we describe the construction of a multiphoton microscope that will enable the study of both fluorescently labeled and unlabeled tissue. This has been used to monitor the contribution of Wt1 expressing cells towards the visceral fat depots during gestation. PMID:27417963

  7. Clinical multiphoton endoscopy with FLIM capability

    NASA Astrophysics Data System (ADS)

    Weinigel, Martin; Breunig, Hans Georg; Fischer, Peter; Kellner-Höfer, Marcel; Bückle, Rainer; König, Karsten

    2013-02-01

    Multiphoton endoscopy can be applied for intra-corporeal imaging as well as to examine otherwise hard-to-access tissue areas like chronic wounds. Using high-NA (NA = 0.8) gradient-index (GRIN) lens-based endoscopes with a diameter of 1.4 mm and effective lengths of 7 mm and 20 mm, respectively, two-photon excitation of endogenous fluorophores and second-harmonic generation (SHG) is used for multimodal in vivo imaging of human skin. A further imaging modality is fluorescence lifetime imaging (FLIM) which allows functional imaging to investigate the healing mechanism of chronic wounds and the corresponding cell metabolism. We performed first in vivo measurements using FLIM endoscopy with the medically-certified multiphoton tomograph MPTflex® in combination with a computer-controlled motorized scan head and a GRIN-lens endoscope.

  8. Multiphoton tomography to detect chemo- and biohazards

    NASA Astrophysics Data System (ADS)

    König, Karsten

    2015-03-01

    In vivo high-resolution multiphoton/CARS tomography provides optical biopsies with 300 nm lateral resolution with chemical fingerprints. Thousands of volunteers and patients have been investigated for early cancer diagnosis, evaluation of anti-ageing cosmetic products, and changes of cellular metabolism by UV exposure and decreased oxygen supply. The skin as the outermost and largest organ is also the major target of CB agents. Current UV-based sensors are useful for bio-aerosol sensing but not for evaluating exposed in vivo skin. Here we evaluate the use of 4D multiphoton/CARS tomographs based on near infrared femtosecond laser radiation, time-correlated single photon counting (FLIM) and white light generation by photonic crystal fibers to detect bio- and chemohazards in human in vivo skin using twophoton fluorescence, SHG, and Raman signals.

  9. First multiphoton tomography of brain in man

    NASA Astrophysics Data System (ADS)

    König, Karsten; Kantelhardt, Sven R.; Kalasauskas, Darius; Kim, Ella; Giese, Alf

    2016-03-01

    We report on the first two-photon in vivo brain tissue imaging study in man. High resolution in vivo histology by multiphoton tomography (MPT) including two-photon FLIM was performed in the operation theatre during neurosurgery to evaluate the feasibility to detect label-free tumor borders with subcellular resolution. This feasibility study demonstrates, that MPT has the potential to identify tumor borders on a cellular level in nearly real-time.

  10. Medium-induced multi-photon radiation

    NASA Astrophysics Data System (ADS)

    Ma, Hao; Salgado, Carlos A.; Tywoniuk, Konrad

    2011-01-01

    We study the spectrum of multi-photon radiation off a fast quark in medium in the BDMPS/ASW approach. We reproduce the medium-induced one-photon radiation spectrum in dipole approximation, and go on to calculate the two-photon radiation in the Molière limit. We find that in this limit the LPM effect holds for medium-induced two-photon ladder emission.

  11. Multiphoton microscopy with near infrared contrast agents

    NASA Astrophysics Data System (ADS)

    Yazdanfar, Siavash; Joo, Chulmin; Zhan, Chun; Berezin, Mikhail Y.; Akers, Walter J.; Achilefu, Samuel

    2010-05-01

    While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

  12. Fundamental studies of molecular multiphoton ionization

    SciTech Connect

    Miller, J.C.; Compton, R.N.

    1984-04-01

    For several years the authors have performed fundamental studies of molecular multiphoton ionization (MPI). We will present a potpourri of techniques and results chosen to illustrate the interesting complexities of molecular MPI. Techniques used include time-of-flight mass spectroscopy, photoelectron spectroscopy, supersonic expansion cooling of molecular beams, harmonic generation, two-color laser MPI, and polarization spectroscopy. Whenever possible the relevance of these results to resonance ionization spectroscopy schemes will be delineated. 23 references, 10 figures.

  13. Optimization-based wavefront sensorless adaptive optics for multiphoton microscopy.

    PubMed

    Antonello, Jacopo; van Werkhoven, Tim; Verhaegen, Michel; Truong, Hoa H; Keller, Christoph U; Gerritsen, Hans C

    2014-06-01

    Optical aberrations have detrimental effects in multiphoton microscopy. These effects can be curtailed by implementing model-based wavefront sensorless adaptive optics, which only requires the addition of a wavefront shaping device, such as a deformable mirror (DM) to an existing microscope. The aberration correction is achieved by maximizing a suitable image quality metric. We implement a model-based aberration correction algorithm in a second-harmonic microscope. The tip, tilt, and defocus aberrations are removed from the basis functions used for the control of the DM, as these aberrations induce distortions in the acquired images. We compute the parameters of a quadratic polynomial that is used to model the image quality metric directly from experimental input-output measurements. Finally, we apply the aberration correction by maximizing the image quality metric using the least-squares estimate of the unknown aberration. PMID:24977374

  14. Multiphoton harvesting metal–organic frameworks

    PubMed Central

    Quah, Hong Sheng; Chen, Weiqiang; Schreyer, Martin K.; Yang, Hui; Wong, Ming Wah; Ji, Wei; Vittal, Jagadese J.

    2015-01-01

    Multiphoton upconversion is a process where two or more photons are absorbed simultaneously to excite an electron to an excited state and, subsequently, the relaxation of electron gives rise to the emission of a photon with frequency greater than those of the absorbed photons. Materials possessing such property attracted attention due to applications in biological imaging, photodynamic therapy, three-dimensional optical data storage, frequency-upconverted lasing and optical power limiting. Here we report four-photon upconversion in metal–organic frameworks containing the ligand, trans, trans-9,10-bis(4-pyridylethenyl)anthracene. The ligand has a symmetrical acceptor–π–donor–π–acceptor structure and a singlet biradical electronic ground state, which boosted its multiphoton absorption cross-sections. We demonstrate that the upconversion efficiency can be enhanced by Förster resonance energy transfer within host–guest metal–organic frameworks consisting of encapsulated high quantum yielding guest molecules. Using these strategies, metal–organic framework materials, which can exhibit frequency-upconverted photoluminescence excited by simultaneous multiphoton absorption, can be rationally designed and synthesized. PMID:26245741

  15. Multiphoton harvesting metal-organic frameworks

    NASA Astrophysics Data System (ADS)

    Quah, Hong Sheng; Chen, Weiqiang; Schreyer, Martin K.; Yang, Hui; Wong, Ming Wah; Ji, Wei; Vittal, Jagadese J.

    2015-08-01

    Multiphoton upconversion is a process where two or more photons are absorbed simultaneously to excite an electron to an excited state and, subsequently, the relaxation of electron gives rise to the emission of a photon with frequency greater than those of the absorbed photons. Materials possessing such property attracted attention due to applications in biological imaging, photodynamic therapy, three-dimensional optical data storage, frequency-upconverted lasing and optical power limiting. Here we report four-photon upconversion in metal-organic frameworks containing the ligand, trans, trans-9,10-bis(4-pyridylethenyl)anthracene. The ligand has a symmetrical acceptor-π-donor-π-acceptor structure and a singlet biradical electronic ground state, which boosted its multiphoton absorption cross-sections. We demonstrate that the upconversion efficiency can be enhanced by Förster resonance energy transfer within host-guest metal-organic frameworks consisting of encapsulated high quantum yielding guest molecules. Using these strategies, metal-organic framework materials, which can exhibit frequency-upconverted photoluminescence excited by simultaneous multiphoton absorption, can be rationally designed and synthesized.

  16. Visualization of in vivo thromboprophylactic and thrombolytic efficacy of enoxaparin in laser-induced vascular endothelial injury model using multiphoton microscopy

    PubMed Central

    Tanaka, Koji; Koike, Yuhki; Matsushita, Kohei; Okigami, Masato; Toiyama, Yuji; Kawamura, Mikio; Saigusa, Susumu; Okugawa, Yoshinaga; Inoue, Yasuhiro; Uchida, Keiichi; Araki, Toshimitsu; Mohri, Yasuhiko; Mizoguchi, Akira; Kusunoki, Masato

    2015-01-01

    Enoxaparin is used postoperatively for the prevention of venous thromboembolism. In vitro studies and clinical trials have demonstrated the anticoagulant and antithrombotic efficacy of enoxaparin. In this study, we visualised thromboprophylactic and thrombolytic efficacy of enoxaparin in a laser-induced thrombus formation model in vivo using two-photon laser-scanning microscopy (TPLSM). Thrombus was induced by the selective irradiation of vascular endothelium in arterioles of the cecum of green fluorescent protein transgenic mice. The thromboprophylactic and thrombolytic efficacy of enoxaparin was visualised in vivo real-time using TPLSM. Platelet adhesion, aggregation, and platelet-dependent thrombus formation were observed in the laser-induced thrombus formation model with reproducibility. Laser-induced thrombus formation was significantly inhibited by enoxaparin pretreatment as the thromboprophylactic agent, as compared with control. The mean thrombus volumes were 652 microcubic meters in mice pretreated with enoxaparin and 8906 microcubic meter in control mice. Enoxaparin reduced the volume of laser-induced thrombus when using it as a thrombolytic agent. The mean rate of reduction was 59 percent. In a lipopolysaccharide-induced sepsis model, thromboprophylactic efficacy of enoxaparin was also observed in vivo in real-time. In vivo thromboprophylactic and thrombolytic efficacy of enoxaparin can be visualised at the single platelet level in the laser-induced endothelium injury model using TPLSM. PMID:25755830

  17. Some simple mechanisms of multiphoton excitation in many - level systems

    NASA Astrophysics Data System (ADS)

    Donley, E. A.; Marquardt, R.; Quack, M.; Stohner, J.; Thanopulos, I.; Wallenborn, E.-U.

    Results are reported on coherent monochromatic multiphoton excitation in many-level systems, which are representative for some of the basic mechanisms for atomic and molecular multiphoton processes. Numerical solutions are discussed that use the Floquet and quasiresonant approximations in the framework of the URIMIR program package. The excitation schemes include direct three-photon excitation, two-photon excitation with diagonal coupling, Göppert-Mayer-type two-photon processes, multiphoton excitation with off-resonant intermediates, and practically irreversible coherent excitation into dense spectral structures. Several interesting phenomena are observed, such as nonlinear line shifts and broadenings of multiphoton resonances of relevance for multiphoton spectroscopy and almost constant intermediate population inversions, potentially useful for laser design. The accurate numerical results are compared with approximate solutions from perturbation theory, and with simple analytical solutions from Rabi-type formulae.

  18. Multi-photon absorption limits to heralded single photon sources

    PubMed Central

    Husko, Chad A.; Clark, Alex S.; Collins, Matthew J.; De Rossi, Alfredo; Combrié, Sylvain; Lehoucq, Gaëlle; Rey, Isabella H.; Krauss, Thomas F.; Xiong, Chunle; Eggleton, Benjamin J.

    2013-01-01

    Single photons are of paramount importance to future quantum technologies, including quantum communication and computation. Nonlinear photonic devices using parametric processes offer a straightforward route to generating photons, however additional nonlinear processes may come into play and interfere with these sources. Here we analyse spontaneous four-wave mixing (SFWM) sources in the presence of multi-photon processes. We conduct experiments in silicon and gallium indium phosphide photonic crystal waveguides which display inherently different nonlinear absorption processes, namely two-photon (TPA) and three-photon absorption (ThPA), respectively. We develop a novel model capturing these diverse effects which is in excellent quantitative agreement with measurements of brightness, coincidence-to-accidental ratio (CAR) and second-order correlation function g(2)(0), showing that TPA imposes an intrinsic limit on heralded single photon sources. We build on these observations to devise a new metric, the quantum utility (QMU), enabling further optimisation of single photon sources. PMID:24186400

  19. Multiphoton intravital microscopy setup to visualize the mouse mammary gland

    NASA Astrophysics Data System (ADS)

    Adur, Javier; Herrera Torres, Ana M.; Masedunskas, Andrius; Baratti, Mariana O.; de Thomaz, Andre A.; Pelegati, Vitor B.; Carvalho, Hernandes F.; Cesar, Carlos L.

    2013-06-01

    Recently, light microscopy-based techniques have been extended to live mammalian models leading to the development of a new imaging approach called intravital microscopy (IVM). Although IVM has been introduced at the beginning of the last century, its major advancements have occurred in the last twenty years with the development of non-linear microscopy that has enabled performing deep tissue imaging. IVM has been utilized to address many biological questions in basic research and is now a fundamental tool that provide information on tissues such as morphology, cellular architecture, and metabolic status. IVM has become an indispensable tool in numerous areas. This study presents and describes the practical aspects of IVM necessary to visualize epithelial cells of live mouse mammary gland with multiphoton techniques.

  20. High-Resolution Multiphoton Imaging of Tumors In Vivo

    PubMed Central

    Wyckoff, Jeffrey; Gligorijevic, Bojana; Entenberg, David; Segall, Jeffrey; Condeelis, John

    2014-01-01

    Analysis of the individual steps in metastasis is crucial if insights at the molecular level are to be linked to the cell biology of cancer. A technical hurdle to achieving the analysis of the individual steps of metastasis is the fact that, at the gross level, tumors are heterogeneous in both animal models and patients. Human primary tumors show extensive variation in all properties ranging from growth and morphology of the tumor through tumor-cell density in the blood and formation and growth of metastases. Methods capable of the direct visualization and analysis of tumor-cell behavior at single-cell resolution in vivo have become crucial in advancing the understanding of mechanisms of metastasis, the definition of microenvironment, and the markers related to both. This article discusses the use of high-resolution multiphoton imaging of tumors (specifically breast tumors in mice) in vivo. PMID:21969629

  1. Multiphoton and tunneling ionization probability of atoms and molecules in an intense laser field

    NASA Astrophysics Data System (ADS)

    Zhao, Song-Feng; Liu, Lu; Zhou, Xiao-Xin

    2014-02-01

    We theoretically studied ionization of atoms exposed to an intense laser field by using three different methods, i.e., the numerical solution of the single-active-electron approximation based time-dependent Schrödinger equation (SAE-TDSE), the Perelomov-Popov-Terent'ev (PPT) model, and the Ammosov-Delone-Krainov (ADK) model. The ionization of several linear molecules in a strong laser field is also investigated with the molecular ADK (MO-ADK) and the molecular PPT (MO-PPT) model. We show that the ionization probability from the PPT and the MO-PPT model agrees well with the corresponding SAE-TDSE result in both the multiphoton and tunneling ionization regimes. By considering the volume effect of the laser field, the ionization signal obtained from the PPT and the MO-PPT model fits well the experimental data in the whole range of the multiphoton and tunneling ionization regimes. However, both the ADK and MO-ADK models seriously underestimate the ionization probabilities (or signals) in the multiphoton regime.

  2. Polymer microcantilevers fabricated via multiphoton absorption polymerization

    NASA Astrophysics Data System (ADS)

    Bayindir, Z.; Sun, Y.; Naughton, M. J.; LaFratta, C. N.; Baldacchini, T.; Fourkas, J. T.; Stewart, J.; Saleh, B. E. A.; Teich, M. C.

    2005-02-01

    We have used multiphoton absorption polymerization to fabricate a series of microscale polymer cantilevers. Atomic force microscopy has been used to characterize the mechanical properties of microcantilevers with spring constants that were found to span more than four decades. From these data, we extracted a Young's modulus of E =0.44GPa for these microscale cantilevers. The wide stiffness range and relatively low elastic modulus of the microstructures make them attractive candidates for a range of microcantilever applications, including measurements on soft matter.

  3. Pulse front adaptive optics in multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Sun, B.; Salter, P. S.; Booth, M. J.

    2016-03-01

    The accurate focusing of ultrashort laser pulses is extremely important in multiphoton microscopy. Using adaptive optics to manipulate the incident ultrafast beam in either the spectral or spatial domain can introduce significant benefits when imaging. Here we introduce pulse front adaptive optics: manipulating an ultrashort pulse in both the spatial and temporal domains. A deformable mirror and a spatial light modulator are operated in concert to modify contours of constant intensity in space and time within an ultrashort pulse. Through adaptive control of the pulse front, we demonstrate an enhancement in the measured fluorescence from a two photon microscope.

  4. Widefield multiphoton excited fluorescence microscopy for animal study in vivo

    NASA Astrophysics Data System (ADS)

    Cheng, L.-C.; Chang, C.-Y.; Lin, C.-H.; Su, Y.-D.; Huang, T.-Y.; Chen, S.-J.

    2010-08-01

    Unlike conventional multiphoton excited microscopy according to pixel-by-pixel point scanning, a widefield multiphoton excited microscopy based on spatiotemporal focusing has been developed to construct three-dimensional (3D) multiphoton fluorescence images only with the need of an axial scanning. By implementing a 4.0 W 10 kHz femtosecond laser amplifier with an instant strong peak power and a fast TE-cooled EMCCD camera with an ultra-sensitive fluorescence detection, the multiphoton excited fluorescence images with the excitation area over 100 μm x 100 μm can be achieved at a frame rate up to 80 Hz. A mechanical shutter is utilized to control the exposure time of 1 ms, i.e. average ten laser pulses reach the fluorescent specimen, and hence an uniform enough multiphoton excited fluorescence image can be attained with less photobleaching. The Brownian motion of microbeads and 3D neuron cells of a rat cerebellum have been observed with a lateral spatial resolution of 0.24 μm and an axial resolution of 2.5 μm. Therefore, the developed widefield multiphoton microscopy can provide fast and high-resolution multiphoton excited fluorescence images for animal study in vivo.

  5. Development of an applicator for multiphoton PDT

    NASA Astrophysics Data System (ADS)

    Graschew, Georgi; Bastian, Matthias; Rakowsky, Stefan; Roelofs, Theo A.; Balanos, Evangelos; Schlag, Peter M.; Steinmeyer, Gunter; Elsaesser, Thomas

    2004-09-01

    Multiphoton excitation of photosensitizers for laser induced fluorescence diagnosis (LIFD) and photodynamic therapy (PDT) of tumors has the advantage of greater tissue penetration due to the longer wavelength of irradiation. However, multiphoton LIFD and PDT are presently not clinically applicable as there are no applicators available for the delivery of the pulsed laser radiation to the operating room. As an approach, in this contribution the beam delivery through photonic crystal fibers has been investigated. Pulses of a Ti:sapphire laser of 100 fs pulse duration and an average power of 150 mW have been transported through such a fiber of 25 m length and the resulting pulses show the absence of nonlinear contributions but still a broadening of the pulse to 2 ps due to the dispersion of the fiber. It is planned to compensate this broadening by a grating in front of the fiber. Alternatively, the transport of laser radiation of 150 fs and 100 mW through a mirror-joint-arm used for conventional CO2 lasers has been tested showing no broadening of the laser pulses. Two-photon photodynamic activity of mTHPC-CMPEG4 shall serve as a test of the laser light transport system.

  6. Multiphoton ionization of large water clusters

    SciTech Connect

    Apicella, B.; Li, X.; Passaro, M.; Spinelli, N.; Wang, X.

    2014-05-28

    Water clusters are multimers of water molecules held together by hydrogen bonds. In the present work, multiphoton ionization in the UV range coupled with time of flight mass spectrometry has been applied to water clusters with up to 160 molecules in order to obtain information on the electronic states of clusters of different sizes up to dimensions that can approximate the bulk phase. The dependence of ion intensities of water clusters and their metastable fragments produced by laser ionization at 355 nm on laser power density indicates a (3+1)-photon resonance-enhanced multiphoton ionization process. It also explains the large increase of ionization efficiency at 355 nm compared to that at 266 nm. Indeed, it was found, by applying both nanosecond and picosecond laser ionization with the two different UV wavelengths, that no water cluster sequences after n = 9 could be observed at 266 nm, whereas water clusters up to m/z 2000 Th in reflectron mode and m/z 3000 Th in linear mode were detected at 355 nm. The agreement between our findings on clusters of water, especially true in the range with n > 10, and reported data for liquid water supports the hypothesis that clusters above a critical dimension can approximate the liquid phase. It should thus be possible to study clusters just above 10 water molecules, for getting information on the bulk phase structure.

  7. The infrared multiphoton dissociation of three nitrolkanes

    NASA Astrophysics Data System (ADS)

    Wodtke, A. M.; Hintsa, E. J.; Lee, Y. T.

    1986-01-01

    Infrared multiphoton dissociation in a molecular beam has been studied in order to elucidate the collision free, 'thermal' chemistry and dynamics of nitromethane, nitroethane and 2-nitropropane. The isomerization of CH3NO2 to CH3ONO was observed by detecting the CH3O and NO products from the dissociation of the very internally hot, isomerized nitromethane. A novel application of RRKM theory was used to estimate the barrier height to isomerization at 55.5 kcal/mol. The barrier height determination method was tested and found to give excellent results by applying it to the determintaion of the barrier height to HONO elimination from nitroethane, a value which is well known from activation energy measurements. The method was then applied to the case of HONO elimination from 2-nitropropane and it appears that there is good to believe that the barrier height is 3-5 kcal/mol lower in 2-nitropropane than in nitroethane. The success of this method for determining barrier heights shows how a microscopic molecular beam experiment, using infrared multiphoton dissociation where the concept of temperature has no place, can be quantitatively related to pyrolysis experiments which are conducted under collisional, thermal conditions and measure phenomenological quantities such as activation energies.

  8. Multimodal optoacoustic and multiphoton fluorescence microscopy

    NASA Astrophysics Data System (ADS)

    Sela, Gali; Razansky, Daniel; Shoham, Shy

    2013-03-01

    Multiphoton microscopy is a powerful imaging modality that enables structural and functional imaging with cellular and sub-cellular resolution, deep within biological tissues. Yet, its main contrast mechanism relies on extrinsically administered fluorescent indicators. Here we developed a system for simultaneous multimodal optoacoustic and multiphoton fluorescence 3D imaging, which attains both absorption and fluorescence-based contrast by integrating an ultrasonic transducer into a two-photon laser scanning microscope. The system is readily shown to enable acquisition of multimodal microscopic images of fluorescently labeled targets and cell cultures as well as intrinsic absorption-based images of pigmented biological tissue. During initial experiments, it was further observed that that detected optoacoustically-induced response contains low frequency signal variations, presumably due to cavitation-mediated signal generation by the high repetition rate (80MHz) near IR femtosecond laser. The multimodal system may provide complementary structural and functional information to the fluorescently labeled tissue, by superimposing optoacoustic images of intrinsic tissue chromophores, such as melanin deposits, pigmentation, and hemoglobin or other extrinsic particle or dye-based markers highly absorptive in the NIR spectrum.

  9. Multifocal multiphoton microscopy with adaptive optical correction

    NASA Astrophysics Data System (ADS)

    Coelho, Simao; Poland, Simon; Krstajic, Nikola; Li, David; Monypenny, James; Walker, Richard; Tyndall, David; Ng, Tony; Henderson, Robert; Ameer-Beg, Simon

    2013-02-01

    Fluorescence lifetime imaging microscopy (FLIM) is a well established approach for measuring dynamic signalling events inside living cells, including detection of protein-protein interactions. The improvement in optical penetration of infrared light compared with linear excitation due to Rayleigh scattering and low absorption have provided imaging depths of up to 1mm in brain tissue but significant image degradation occurs as samples distort (aberrate) the infrared excitation beam. Multiphoton time-correlated single photon counting (TCSPC) FLIM is a method for obtaining functional, high resolution images of biological structures. In order to achieve good statistical accuracy TCSPC typically requires long acquisition times. We report the development of a multifocal multiphoton microscope (MMM), titled MegaFLI. Beam parallelization performed via a 3D Gerchberg-Saxton (GS) algorithm using a Spatial Light Modulator (SLM), increases TCSPC count rate proportional to the number of beamlets produced. A weighted 3D GS algorithm is employed to improve homogeneity. An added benefit is the implementation of flexible and adaptive optical correction. Adaptive optics performed by means of Zernike polynomials are used to correct for system induced aberrations. Here we present results with significant improvement in throughput obtained using a novel complementary metal-oxide-semiconductor (CMOS) 1024 pixel single-photon avalanche diode (SPAD) array, opening the way to truly high-throughput FLIM.

  10. Multiphoton imaging with a nanosecond supercontinuum source

    NASA Astrophysics Data System (ADS)

    Lefort, Claire; O'Connor, Rodney P.; Blanquet, Véronique; Baraige, Fabienne; Tombelaine, Vincent; Lévêque, Philippe; Couderc, Vincent; Leproux, Philippe

    2016-03-01

    Multiphoton microscopy is a well-established technique for biological imaging of several kinds of targets. It is classically based on multiphoton processes allowing two means of contrast simultaneously: two-photon fluorescence (TPF) and second harmonic generation (SHG). Today, the quasi exclusive laser technology used in that aim is femtosecond titanium sapphire (Ti: Sa) laser. We experimentally demonstrate that a nanosecond supercontinuum laser source (STM-250-VIS-IR-custom, Leukos, France; 1 ns, 600-2400 nm, 250 kHz, 1 W) allows to obtain the same kind of image quality in the case of both TPF and SHG, since it is properly filtered. The first set of images concerns the muscle of a mouse. It highlights the simultaneous detection of TPF and SHG. TPF is obtained thanks to the labelling of alpha-actinin with Alexa Fluor® 546 by immunochemistry. SHG is created from the non-centrosymmetric organization of myosin. As expected, discs of actin and myosin are superimposed alternatively. The resulting images are compared with those obtained from a standard femtosecond Ti: Sa source. The physical parameters of the supercontinuum are discussed. Finally, all the interest of using an ultra-broadband source is presented with images obtained in vivo on the brain of a mouse where tumor cells labeled with eGFP are grafted. Texas Red® conjugating Dextran is injected into the blood vessels network. Thus, two fluorophores having absorption wavelengths separated by 80 nm are imaged simultaneously with a single laser source.

  11. Controllable infrared continuum source for multiphoton imaging

    NASA Astrophysics Data System (ADS)

    de Mauro, C.; Alfieri, D.; Arrigoni, M.; Armstrong, D.; Pavone, F. S.

    2010-02-01

    We report on multiphoton imaging of biological samples performed with continuum infrared source generated in photonic crystal fibers (PCFs). We studied the spectra generated in PCFs with dispersion profiles designed to maximize the power density in the 700-1000 nm region, where the two-photon absorption cross sections of the most common dyes lie. Pumping in normal dispersion region, with <140 femtosecond pulses delivered by a tunable Ti:Sa laser (Chameleon Ultra II by Coherent Inc.), results in a limitation of nonlinear broadening up to a mean power density above 2 mW/nm. Axial and lateral resolution obtained with a scanning multiphoton system has been measureed to be near the theoretical limit. The possibility of simultaneous two-photon excitation of different dyes in the same sample and high image resolution are demonstrated at tens of microns in depth. Signal-to-noise ratio and general performances are found to be comparable with those of a single wavelength system, used for comparison.

  12. Statistical properties of multiphoton time-dependent three-boson coupled oscillators

    SciTech Connect

    Abdalla, M. Sebawe; Perina, Jan; Krepelka, Jaromir

    2006-06-15

    We investigate the quantum statistics of three time-dependent coupled oscillators in the presence of multiphoton processes. The system is connected with the two-atom multiphoton Tavis-Cummings model. The solution of the Heisenberg equations of the motion is obtained in a compact form. We assume that the modes are initially prepared in coherent states, and we discuss nonclassical phenomena (squeezing and sub-Poissonian behavior). Further, we examine the joint quasi-distribution functions as well as photon-number distribution and its factorial moments. The system has shown that the nonclassical effect is apparent in compound modes (1,3) and (2,3). Moreover, the superstructure phenomenon is observed when the photon transition is increased.

  13. Multiphoton fluorescence imaging of NADH to quantify metabolic changes in epileptic tissue in vitro

    NASA Astrophysics Data System (ADS)

    Chia, Thomas H.; Zinter, Joseph; Spencer, Dennis D.; Williamson, Anne; Levene, Michael J.

    2007-02-01

    A powerful advantage of multiphoton microscopy is its ability to image endogenous fluorophores such as the ubiquitous coenzyme NADH in discrete cellular populations. NADH is integral in both oxidative and non-oxidative cellular metabolism. NADH loses fluorescence upon oxidation to NAD +; thus changes in NADH fluorescence can be used to monitor metabolism. Recent studies have suggested that hypo metabolic astrocytes play an important role in cases of temporal lobe epilepsy (TLE). Current theories suggest this may be due to defective and/or a reduced number of mitochondria or dysfunction of the neuronal-astrocytic metabolic coupling. Measuring NADH fluorescence changes following chemical stimulation enables the quantification of the cellular distribution of metabolic anomalies in epileptic brain tissue compared to healthy tissue. We present what we believe to be the first multiphoton microscopy images of NADH from the human brain. We also present images of NADH fluorescence from the hippocampus of the kainate-treated rat TLE model. In some experiments, human and rat astrocytes were selectively labeled with the fluorescent dye sulforhodamine 101 (SR101). Our results demonstrate that multiphoton microscopy is a powerful tool for assaying the metabolic pathologies associated with temporal lobe epilepsy in humans and in rodent models.

  14. Weak-field multiphoton femtosecond coherent control in the single-cycle regime.

    PubMed

    Chuntonov, Lev; Fleischer, Avner; Amitay, Zohar

    2011-03-28

    Weak-field coherent phase control of atomic non-resonant multiphoton excitation induced by shaped femtosecond pulses is studied theoretically in the single-cycle regime. The carrier-envelope phase (CEP) of the pulse, which in the multi-cycle regime does not play any control role, is shown here to be a new effective control parameter that its effect is highly sensitive to the spectral position of the ultrabroad spectrum. Rationally chosen position of the ultrabroadband spectrum coherently induces several groups of multiphoton transitions from the ground state to the excited state of the system: transitions involving only absorbed photons as well as Raman transitions involving both absorbed and emitted photons. The intra-group interference is controlled by the relative spectral phase of the different frequency components of the pulse, while the inter-group interference is controlled jointly by the CEP and the relative spectral phase. Specifically, non-resonant two- and three-photon excitation is studied in a simple model system within the perturbative frequency-domain framework. The developed intuition is then applied to weak-field multiphoton excitation of atomic cesium (Cs), where the simplified model is verified by non-perturbative numerical solution of the time-dependent Schrödinger equation. We expect this work to serve as a basis for a new line of femtosecond coherent control experiments. PMID:21451714

  15. First-principles calculation of multiphoton absorption cross section of α-quartz under femtosecond laser irradiation

    NASA Astrophysics Data System (ADS)

    Yu, Dong; Jiang, Lan; Wang, Feng; Qu, Liangti; Lu, Yongfeng

    2016-05-01

    Time-dependent density functional theory-based first-principles calculations have been used to study the ionization process and electron excitation. The results show that the number of excited electrons follows the power law σ k I k at peak intensities of I < 5 × 1013 W/cm2, indicating that the multiphoton ionization plays a key role. The multiphoton absorption cross section of α-quartz σ k is further calculated to be 3.54 × 1011 cm-3 ps-1 (cm2/TW)6. Using the plasma model, the theoretical results of the damage threshold fluences are consistent with the experimental data, which validates the calculated value of multiphoton absorption cross section. By employing the calculated cross section value in the plasma model, the damage threshold fluences are theoretically estimated, being consistent with the experimental data, which validates the calculated value of multiphoton absorption cross section. The preliminary multiscale model shows great potential in the simulation of laser processing.

  16. In vivo multiphoton tomography of inflammatory tissue and melanoma

    NASA Astrophysics Data System (ADS)

    Riemann, Iris; Dimitrow, Enrico; Kaatz, Martin; Fluhr, Joachim; Elsner, Peter; Kobow, Jens; Konig, Karsten

    2005-04-01

    Multiphoton optical tomography provides the capability of non-invasive optical sectioning of skin with high spatial and intracellular resolution as well as high NIR (near infrared) light penetration into pigmented skin areas. The imaging system DermaInspect based on femtosecond laser pulses was used to perform multiphoton optical tomography in clinical studies. Patients with abnormal pigmented tissues were imaged in vivo. After the multiphoton imaging procedure, biopsies were taken, imaged again and further processed with standard histological methods. We report on preliminary results. The visualization of pigmented cell clusters based on non-linear luminescence using the novel multiphoton device was possible. These clusters could be clearly distinguished from non-pigmented cells. Cancerous tissues showed significant differences in the cell structure of the epidermal layers. The system DermaInspect might become a high resolution diagnostic tool for melanoma diagnostics.

  17. Multiphoton imaging of biological samples during freezing and heating

    NASA Astrophysics Data System (ADS)

    Breunig, H. G.; Uchugonova, A.; König, K.

    2014-02-01

    We applied multiphoton microscopic imaging to observe freezing and heating effects in plant- and animal cell samples. The experimental setups consisted of a multiphoton imaging system and a heating and cooling stage which allows for precise temperature control from liquid nitrogen temperature (-196°C 77 K) up to +600°C (873 K) with heating/freezing rates between 0.01 K/min and 150 K/min. Two multiphoton imaging systems were used: a system based on a modified optical microscope and a flexible mobile system. To illustrate the imaging capabilities, plant leafs as well as animal cells were microscopically imaged in vivo during freezing based on autofluorescence lifetime and intensity of intrinsic molecules. The measurements illustrate the usefulness of multiphoton imaging to investigate freezing effects on animal and plant cells.

  18. Multiphoton nanosurgery in cells and tissues

    NASA Astrophysics Data System (ADS)

    Riemann, Iris; Anhut, Tiemo; Stracke, Frank; Le Harzic, Ronan; Koenig, Karsten

    2005-04-01

    Multiphoton Microscopy with a femtosecond pulsed Ti:sapphire laser in the near infrared (NIR) enables the user not only to image cells and tissues with a subcellular resolution but also to perform highly precise nanosurgery. Intratissue compartments, single cells and even cell organelles like mitochondria, membranes or chromosomes can be manipulated and optically knocked out. Working at transient TW/cm2 laser intensities, single cells of tumor-sphaeroids were eliminated efficiently inside the sphaeroid without damaging the neighbour cells. Also single organelles of cells inside tissues could be optically knocked out with the nanoscalpel without collateral damage. Tissue structures inside a human tooth have been ablated with sizes below 1 μm. This method may become a useful instrument for nano-manipulating and surgery in several fields of science, including targeted transfection.

  19. Multi-photon entanglement in high dimensions

    NASA Astrophysics Data System (ADS)

    Malik, Mehul; Erhard, Manuel; Huber, Marcus; Krenn, Mario; Fickler, Robert; Zeilinger, Anton

    2016-04-01

    Forming the backbone of quantum technologies today, entanglement has been demonstrated in physical systems as diverse as photons, ions and superconducting circuits. Although steadily pushing the boundary of the number of particles entangled, these experiments have remained in a two-dimensional space for each particle. Here we show the experimental generation of the first multi-photon entangled state where both the number of particles and dimensions are greater than two. Two photons in our state reside in a three-dimensional space, whereas the third lives in two dimensions. This asymmetric entanglement structure only appears in multiparticle entangled states with d > 2. Our method relies on combining two pairs of photons, high-dimensionally entangled in their orbital angular momentum. In addition, we show how this state enables a new type of ‘layered’ quantum communication protocol. Entangled states such as these serve as a manifestation of the complex dance of correlations that can exist within quantum mechanics.

  20. REVIEW ARTICLE Multiphoton polymerization of hybrid materials

    NASA Astrophysics Data System (ADS)

    Farsari, Maria; Vamvakaki, Maria; Chichkov, Boris N.

    2010-12-01

    Multiphoton polymerization has been developed as a direct laser writing technique for the preparation of complex 3D structures with resolution beyond the diffraction limit of light. The combination of two or more hybrid materials with different functionalities in the same system has allowed the preparation of structures with advanced properties and functions. Furthermore, the surface functionalization of the 3D structures opens new avenues for their applications in a variety of nanobiotechnological fields. This paper describes the principles of 2PP and the experimental set-up used for 3D structure fabrication. It also gives an overview of the materials that have been employed in 2PP so far and depicts the perspectives of this technique in the development of new active components.

  1. Multiphoton microscopy of cleared mouse organs

    NASA Astrophysics Data System (ADS)

    Parra, Sonia G.; Chia, Thomas H.; Zinter, Joseph P.; Levene, Michael J.

    2010-05-01

    Typical imaging depths with multiphoton microscopy (MPM) are limited to less than 300 μm in many tissues due to light scattering. Optical clearing significantly reduces light scattering by replacing water in the organ tissue with a fluid having a similar index of refraction to that of proteins. We demonstrate MPM of intact, fixed, cleared mouse organs with penetration depths and fields of view in excess of 2 mm. MPM enables the creation of large 3-D data sets with flexibility in pixel format and ready access to intrinsic fluorescence and second-harmonic generation. We present high-resolution images and 3-D image stacks of the brain, small intestine, large intestine, kidney, lung, and testicle with image sizes as large as 4096×4096 pixels.

  2. Enhancing Multiphoton Rates with Quantum Memories

    NASA Astrophysics Data System (ADS)

    Nunn, J.; Langford, N. K.; Kolthammer, W. S.; Champion, T. F. M.; Sprague, M. R.; Michelberger, P. S.; Jin, X.-M.; England, D. G.; Walmsley, I. A.

    2013-03-01

    Single photons are a vital resource for optical quantum information processing. Efficient and deterministic single photon sources do not yet exist, however. To date, experimental demonstrations of quantum processing primitives have been implemented using nondeterministic sources combined with heralding and/or postselection. Unfortunately, even for eight photons, the data rates are already so low as to make most experiments impracticable. It is well known that quantum memories, capable of storing photons until they are needed, are a potential solution to this “scaling catastrophe.” Here, we analyze in detail the benefits of quantum memories for producing multiphoton states, showing how the production rates can be enhanced by many orders of magnitude. We identify the quantity ηB as the most important figure of merit in this connection, where η and B are the efficiency and time-bandwidth product of the memories, respectively.

  3. Point spread function engineering with multiphoton SPIFI

    NASA Astrophysics Data System (ADS)

    Wernsing, Keith A.; Field, Jeffrey J.; Domingue, Scott R.; Allende-Motz, Alyssa M.; DeLuca, Keith F.; Levi, Dean H.; DeLuca, Jennifer G.; Young, Michael D.; Squier, Jeff A.; Bartels, Randy A.

    2016-03-01

    MultiPhoton SPatIal Frequency modulated Imaging (MP-SPIFI) has recently demonstrated the ability to simultaneously obtain super-resolved images in both coherent and incoherent scattering processes -- namely, second harmonic generation and two-photon fluorescence, respectively.1 In our previous analysis, we considered image formation produced by the zero and first diffracted orders from the SPIFI modulator. However, the modulator is a binary amplitude mask, and therefore produces multiple diffracted orders. In this work, we extend our analysis to image formation in the presence of higher diffracted orders. We find that tuning the mask duty cycle offers a measure of control over the shape of super-resolved point spread functions in an MP-SPIFI microscope.

  4. Multiphoton imaging with high peak power VECSELs

    NASA Astrophysics Data System (ADS)

    Mirkhanov, Shamil; Quarterman, Adrian H.; Swift, Samuel; Praveen, Bavishna B.; Smyth, Conor J. C.; Wilcox, Keith G.

    2016-03-01

    Multiphoton imaging (MMPI) has become one of thee key non-invasive light microscopy techniques. This technique allows deep tissue imaging with high resolution and less photo-damage than conventional confocal microscopy. MPI is type of laser-scanning microscopy that employs localized nonlinear excitation, so that fluorescence is excited only with is scanned focal volume. For many years, Ti: sapphire femtosecond lasers have been the leading light sources for MPI applications. However, recent developments in laser sources and new types of fluorophores indicate that longer wavelength excitation could be a good alternative for these applications. Mode-locked VECSEELs have the potential to be low cost, compact light sources for MPI systems, with the additional advantage of broad wavelength coverage through use of different semiconductor material systems. Here, we use a femtosecond fibber laser to investigate the effect average power and repetition rate has on MPI image quality, to allow us to optimize our mode-locked VVECSELs for MPI.

  5. Multiphoton double ionization of the He atom

    NASA Astrophysics Data System (ADS)

    Li, Y.; Pindzola, M. S.

    2016-05-01

    Time-dependent close-coupling (TDCC) calculations are made for the multiphoton double ionization of the He atom under the influence of a fast pulse XUV laser. One set of TDCC calculations employs l1m1l2m2 coupling on a 2D (r1 ,r2) numerical lattice, a second set of TDCC calculations employs m1m2 coupling on a 4D (r1 ,θ1 ,r2 ,θ2) numerical lattice, and a third set of TDCC calculations employs m1m2 coupling on a 4D (ρ1 ,z1 ,ρ2 ,z2) numerical lattice. Studies are made to see which TDCC method is the most efficient at explaining measurements as the number of photons absorbed is increased. Work supported in part by Grants from NASA, NSF, and DOE.

  6. Multiphoton lasing in atomic potassium: Steady-state and dynamic behavior

    SciTech Connect

    Font, J. L.; Fernandez-Soler, J. J.; Vilaseca, R.; Gauthier, Daniel J.

    2005-12-15

    We show theoretically that it is possible to generate laser light based on two-photon and other high-order multiphoton processes when an atomic beam of optically driven potassium atoms crosses a high-finesse optical cavity. We use a rigorous model that takes into account all the atomic substates involved in the optical interactions and is valid for any drive and lasing field intensities. The polarizations of the drive and lasing fields are assumed to be fixed. Stable and unstable laser emission branches are obtained, which are represented as a function of cavity detuning and are analyzed in terms of the fundamental quantum processes yielding them. Closed-curve laser-emission profiles are obtained for multiphoton lasing based on processes involving more than one lasing photon. Two-photon laser emission branches show relatively long segments of stationary emission, combined in general with some segments of nonstationary emission, or with segments of mixture with three-photon emission processes. Rayleigh and hyper-Rayleigh processes can become simultaneously resonant, entailing in such case a large and fast transfer of population from the atomic initial ground sublevel to other ground sublevels with different z components of the total angular momentum. They could be useful in generating multiphoton correlated field states. In all cases the largest laser emission intensities are obtained from the highest-order processes, rather than the lowest. These results open the way to the understanding of experiments performed in the past years and suggest possibilities for more efficient and varied types of multiphoton laser operation.

  7. A single-photon fluorescence and multi-photon spectroscopic study of atherosclerotic lesions

    NASA Astrophysics Data System (ADS)

    Smith, Michael S. D.; Ko, Alex C. T.; Ridsdale, Andrew; Schattka, Bernie; Pegoraro, Adrian; Hewko, Mark D.; Shiomi, Masashi; Stolow, Albert; Sowa, Michael G.

    2009-06-01

    In this study we compare the single-photon autofluorescence and multi-photon emission spectra obtained from the luminal surface of healthy segments of artery with segments where there are early atherosclerotic lesions. Arterial tissue was harvested from atherosclerosis-prone WHHL-MI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction), an animal model which mimics spontaneous myocardial infarction in humans. Single photon fluorescence emission spectra of samples were acquired using a simple spectrofluorometer set-up with 400 nm excitation. Samples were also investigated using a home built multi-photon microscope based on a Ti:sapphire femto-second oscillator. The excitation wavelength was set at 800 nm with a ~100 femto-second pulse width. Epi-multi-photon spectroscopic signals were collected through a fibre-optics coupled spectrometer. While the single-photon fluorescence spectra of atherosclerotic lesions show minimal spectroscopic difference from those of healthy arterial tissue, the multi-photon spectra collected from atherosclerotic lesions show marked changes in the relative intensity of two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) signals when compared with those from healthy arterial tissue. The observed sharp increase of the relative SHG signal intensity in a plaque is in agreement with the known pathology of early lesions which have increased collagen content.

  8. Intrinsic Indicator of Photodamage during Label-Free Multiphoton Microscopy of Cells and Tissues

    PubMed Central

    Andresen, Elisabeth F.; Geiger, Kathrin D.; Koch, Edmund; Schackert, Gabriele; Steiner, Gerald; Kirsch, Matthias

    2014-01-01

    Multiphoton imaging has evolved as an indispensable tool in cell biology and holds prospects for clinical applications. When addressing endogenous signals such as coherent anti-Stokes Raman scattering (CARS) or second harmonic generation, it requires intense laser irradiation that may cause photodamage. We report that increasing endogenous fluorescence signal upon multiphoton imaging constitutes a marker of photodamage. The effect was studied on mouse brain in vivo and ex vivo, on ex vivo human brain tissue samples, as well as on glioblastoma cells in vitro, demonstrating that this phenomenon is common to a variety of different systems, both ex vivo and in vivo. CARS microscopy and vibrational spectroscopy were used to analyze the photodamage. The development of a standard easy-to-use model that employs rehydrated cryosections allowed the characterization of the irradiation-induced fluorescence and related it to nonlinear photodamage. In conclusion, the monitoring of endogenous two-photon excited fluorescence during label-free multiphoton microscopy enables to estimate damage thresholds ex vivo as well as detect photodamage during in vivo experiments. PMID:25343251

  9. Evidence for Multi-photon transitions between energy levels in a large Current-Biased Magnesium Diboride Josephson Heterojunction

    NASA Astrophysics Data System (ADS)

    Ramos, Roberto; Carabello, Steven; Lambert, Joseph; Cunnane, Daniel; Dai, Wenqing; Chen, Ke; Li, Qi; Xi, Xiaoxing

    2013-03-01

    When photons are strongly coupled to a quantum system, multiphoton transitions can be observed between two energy levels when the quantum energy of the exciting radiation, multiplied by an integer, matches the level spacing. This phenomenon can be observed in Josephson junction qubits exposed to weak microwave radiation at very low temperatures. At microwave resonance, the transition probability of a junction from superconducting to normal state is enhanced and these are used to map multiphoton transitions. We report observation of single- and multi-photon transitions between ground and first excited states in current-biased MgB2 thin film junctions by applying RF with frequencies between 0.5 and 3 Ghz. These large (up to 0.2mm x 0.3 mm) junctions consist of an MgB2 electrode insulated by native oxide from a lead (Pb) or tin (Sn) counter-electrode, and have areas at least 600 times bigger than Nb junctions previously shown to exhibit multiphoton transitions. The data is consistent with theoretical models of junctions behaving in the quantum limit and show anharmonicity of the junction potential when biased near the critical current.

  10. Multiphoton and photothermal imaging of molecular events in cancer

    NASA Astrophysics Data System (ADS)

    Skala, Melissa

    2010-10-01

    Optical techniques are attractive for monitoring disease processes in living tissues because they are relatively cheap, non-invasive and provide a wealth of functional information. Multiphoton microscopy (MPM) and Optical Coherence Tomography (OCT) are two types of three-dimensional optical imaging modalities that have demonstrated great utility in pre-clinical models of disease. These techniques are particularly useful for identifying metabolic and molecular biomarkers in cancer. These biomarkers can be used to identify the mechanisms of tumor growth, and to predict the response of a particular tumor to treatment. Specifically, MPM of the co-enzymes NADH and FAD was used to quantify metabolic changes associated with developing cancers in vivo. This imaging technique exploits intrinsic sources of tissue contrast and thus does not require contrast agents. Ongoing work combines this metabolic imaging technique with vascular imaging to provide a comprehensive picture of oxygen supply and demand with tumor therapy. Molecular signaling represents a third critical component in tumor physiology. To this end we have recently developed photothermal OCT, which combines coherent detection with laser-heated gold nanoparticles to achieve high-resolution molecular contrast at deeper depths than MPM. This multi-functional imaging platform will provide unprecedented insight into oxygen supply and demand, and molecular signaling in response to tumor growth and targeted cancer therapies in pre-clinical models.

  11. Tunneling dynamics in multiphoton ionization and attoclock calibration.

    PubMed

    Klaiber, Michael; Hatsagortsyan, Karen Z; Keitel, Christoph H

    2015-02-27

    The intermediate domain of strong-field ionization between the tunneling and multiphoton regimes is investigated using the strong-field approximation and the imaginary-time method. An intuitive model for the dynamics is developed which describes the ionization process within a nonadiabatic tunneling picture with a coordinate dependent electron energy during the under-the-barrier motion. The nonadiabatic effects in the elliptically polarized laser field induce a transversal momentum shift of the tunneled electron wave packet at the tunnel exit and a delayed appearance in the continuum as well as a shift of the tunneling exit towards the ionic core. The latter significantly modifies the Coulomb focusing during the electron excursion in the laser field after exiting the ionization tunnel. We show that nonadiabatic effects are especially large when the Coulomb field of the ionic core is taken into account during the under-the-barrier motion. The simple man model modified with these nonadiabatic corrections provides an intuitive background for exact theories and has direct implications for the calibration of the attoclock technique. PMID:25768761

  12. High-resolution multimodal clinical multiphoton tomography of skin

    NASA Astrophysics Data System (ADS)

    König, Karsten

    2011-03-01

    This review focuses on multimodal multiphoton tomography based on near infrared femtosecond lasers. Clinical multiphoton tomographs for 3D high-resolution in vivo imaging have been placed into the market several years ago. The second generation of this Prism-Award winning High-Tech skin imaging tool (MPTflex) was introduced in 2010. The same year, the world's first clinical CARS studies have been performed with a hybrid multimodal multiphoton tomograph. In particular, non-fluorescent lipids and water as well as mitochondrial fluorescent NAD(P)H, fluorescent elastin, keratin, and melanin as well as SHG-active collagen has been imaged with submicron resolution in patients suffering from psoriasis. Further multimodal approaches include the combination of multiphoton tomographs with low-resolution wide-field systems such as ultrasound, optoacoustical, OCT, and dermoscopy systems. Multiphoton tomographs are currently employed in Australia, Japan, the US, and in several European countries for early diagnosis of skin cancer, optimization of treatment strategies, and cosmetic research including long-term testing of sunscreen nanoparticles as well as anti-aging products.

  13. Multiphoton excitation of fluorescent DNA base analogs

    NASA Astrophysics Data System (ADS)

    Katilius, Evaldas; Woodbury, Neal W.

    2006-07-01

    Multiphoton excitation was used to investigate properties of the fluorescent DNA base analogs, 2-aminopurine (2AP) and 6-methylisoxanthopterin (6MI). 2-aminopurine, a fluorescent analog of adenine, was excited by three-photon absorption. Fluorescence correlation measurements were attempted to evaluate the feasibility of using three-photon excitation of 2AP for DNA-protein interaction studies. However, high excitation power and long integration times needed to acquire high signal-to-noise fluorescence correlation curves render three-photon excitation FCS of 2AP not very useful for studying DNA base dynamics. The fluorescence properties of 6-methylisoxanthopterin, a guanine analog, were investigated using two-photon excitation. The two-photon absorption cross-section of 6MI was estimated to be about 2.5×10-50 cm4s (2.5 GM units) at 700 nm. The two-photon excitation spectrum was measured in the spectral region from 700 to 780 nm; in this region the shape of the two-photon excitation spectrum is very similar to the shape of single-photon excitation spectrum in the near-UV spectral region. Two-photon excitation of 6MI is suitable for fluorescence correlation measurements. Such measurements can be used to study DNA base dynamics and DNA-protein interactions over a broad range of time scales.

  14. The multiphoton ionization of uranium hexafluoride

    SciTech Connect

    Armstrong, D.P. . UEO Enrichment Technical Operations Div.)

    1992-05-01

    Multiphoton ionization (MPI) time-of-flight mass spectroscopy and photoelectron spectroscopy studies of UF{sub 6} have been conducted using focused light from the Nd:YAG laser fundamental ({lambda}=1064 nm) and its harmonics ({lambda}=532, 355, or 266 nm), as well as other wavelengths provided by a tunable dye laser. The MPI mass spectra are dominated by the singly and multiply charged uranium ions rather than by the UF{sub x}{sup +} fragment ions even at the lowest laser power densities at which signal could be detected. The laser power dependence of U{sup n+} ions signals indicates that saturation can occur for many of the steps required for their ionization. In general, the doubly-charged uranium ion (U{sup 2+}) intensity is much greater than that of the singly-charged uranium ion (U{sup +}). For the case of the tunable dye laser experiments, the U{sup n+} (n = 1- 4) wavelength dependence is relatively unstructured and does not show observable resonance enhancement at known atomic uranium excitation wavelengths. The dominance of the U{sup 2+} ion and the absence or very small intensities of UF{sub x}{sup +} fragments, along with the unsaturated wavelength dependence, indicate that mechanisms may exist other than ionization of bare U atoms after the stepwise photodissociation of F atoms from the parent molecule.

  15. Multiphoton population transfer between rovibrational states of HF

    NASA Astrophysics Data System (ADS)

    Topcu, Turker; Robicheaux, Francis

    2011-05-01

    Efficient population transfer by adiabatically chirping through a multiphoton resonance in microwave driven and impulsively kicked Rydberg atoms has been reported both experimentally and theoretically. Previous work has demonstrated that the physical mechanism responsible for the transition can be viewed as a classical process in phase space as well as a quantum mechanical resonant transition. Here we report on our classical and quantum mechanical simulations in which we have exploited this mechanism to vibrationally excite an HF molecule up to | ν = 4 , J > from its ground state using an intense IR pulse. We compare one-dimensional quantum and classical models where there are no rotational degrees of freedom. We find that for low laser intensities, the transition is classically forbidden although it occurs quantum mechanically through tunneling. We show that for larger peak intensities, the transfer can be looked upon as a classical transition in phase space, similar to that observed in the atomic case. We extend our simulations to fully three-dimensional quantum calculations and investigate the effect of coupling between different rotational pathways. We briefly discuss the effect of thermal averaging over the final J-states. This work was supported by the Office of Basic Energy Sciences, U.S. Department of Energy.

  16. Multiphoton microscopy of antigen presenting cells in experimental cancer therapies

    NASA Astrophysics Data System (ADS)

    Watkins, Simon C.; Papworth, Glenn D.; Spencer, Lori A.; Larregina, Adriana T.; Hackstein, Holger

    2002-06-01

    The absence of effective conventional therapy for most cancer patients justifies the application of novel, experimental approaches. One alternative to conventional cytotoxic agents is a more defined molecular approach for cancer immune treatment; promotion of the immune system specifically to target and eliminate tumor cells on the basis of expression of tumor-associated antigens (TAA). TAA could be presented to T-cells by professional antigen-presenting cells (APC) that generate a more efficient and effective anti-tumor immune response. In fact, it has been well documented that dendritic cells, the most immunologically potent APC, are capable of recognizing, processing and presenting TAA, in turn initiating a specific antitumor immune response. Results from several laboratories and clinical trials suggested significant but still limited efficacy of TAA-pulsed dendritic cells administered to tumor-bearing hosts. Following such delivery, it is fundamentally necessary to dynamically assess cell abundance within the microenvironment of the tumor in the presence of the appropriate therapeutic agent. Multiphoton microscopy was used to assess the trafficking of pulsed dendritic cells and other APC in skin, lymph nodes and brain of several animal tumor models, following different routes of administration.

  17. Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging

    PubMed Central

    Cua, Michelle; Wahl, Daniel J.; Zhao, Yuan; Lee, Sujin; Bonora, Stefano; Zawadzki, Robert J.; Jian, Yifan; Sarunic, Marinko V.

    2016-01-01

    Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems. PMID:27599635

  18. Evaluation of multiphoton effects in down-conversion

    SciTech Connect

    Yoshimi, Kazuyoshi; Koshino, Kazuki

    2010-04-15

    Multiphoton effects in down-conversion are investigated based on the full-quantum multimode formalism by considering a three-level system as a prototype nonlinear system. We analytically derive the three-photon output wave function for two input photons, where one of the two input photons is down-converted and the other one is not. Using this output wave function, we calculate the down-conversion probability, the purity, and the fidelity to evaluate the entanglement between a down-converted photon pair and a non-down-converted photon. It is shown that the saturation effect occurs by multiphoton input and that it affects both the down-conversion probability and the quantum correlation between the down-converted photon pair and the non-down-converted photon. We also reveal the necessary conditions for multiphoton effects to be strong.

  19. Coherence-Gated Sensorless Adaptive Optics Multiphoton Retinal Imaging.

    PubMed

    Cua, Michelle; Wahl, Daniel J; Zhao, Yuan; Lee, Sujin; Bonora, Stefano; Zawadzki, Robert J; Jian, Yifan; Sarunic, Marinko V

    2016-01-01

    Multiphoton microscopy enables imaging deep into scattering tissues. The efficient generation of non-linear optical effects is related to both the pulse duration (typically on the order of femtoseconds) and the size of the focused spot. Aberrations introduced by refractive index inhomogeneity in the sample distort the wavefront and enlarge the focal spot, which reduces the multiphoton signal. Traditional approaches to adaptive optics wavefront correction are not effective in thick or multi-layered scattering media. In this report, we present sensorless adaptive optics (SAO) using low-coherence interferometric detection of the excitation light for depth-resolved aberration correction of two-photon excited fluorescence (TPEF) in biological tissue. We demonstrate coherence-gated SAO TPEF using a transmissive multi-actuator adaptive lens for in vivo imaging in a mouse retina. This configuration has significant potential for reducing the laser power required for adaptive optics multiphoton imaging, and for facilitating integration with existing systems. PMID:27599635

  20. Post conductive keratoplasty visualization of rabbit cornea by multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Lo, Wen; Wang, Tsung-Jen; Hu, Fung-Rong; Dong, Chen-Yuan

    2007-07-01

    Conductive keratoplasty (CK) is a new refractive surgery for presbyopia and hyperopia patients. By applying radio frequency current at the peripheral regions of cornea, collagen, the most abundant composition of corneal stroma, shrinks due to the heat generated. The shrinkage at the periphery alters the corneal architecture and achieves clearer focus for near vision. In this work we use multiphoton microscopy to observe the post surgery structure variation at both submicron resolution and over a large region within the tissue. Since collagen can be induced to generate strong second harmonic generation (SHG) signal, multiphoton excitation provide direct visualization of collagen orientation within corneal stroma. In addition, since the SHG intensity of collagen tissue deteriorates with increasing thermal damage [1-3], our methodology can be used to characterize the extent of corneal stroma damage from the CK procedure. Finally, the influence of CK on the morphology and distribution of keratocytes can also be investigated by detecting multiphoton excited autofluorescence from the cells.

  1. Nonlinear optical imaging characteristics of colonic adenocarcinoma using multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Liu, Nenrong; Chen, Rong; Li, Hongsheng; Chen, Jianxin

    2012-12-01

    Multiphoton microscopy (MPM), a noninvasive optical method with high resolution and high sensitivity, can obtain detailed microstructures of biotissues at submolecular level. In this study, MPM is used to image microstructure varieties of human colonic mucosa and submucosa with adenocarcinoma. Some parameters, such as gland configuration, SHG/TPEF intensity ratio, and collagen orientation and so on, should serve the indicators of early colorectal cancer. The exploratory results show that it's potential for the development of multiphoton mini-endoscopy in real-time early diagnosis of colorectal cancer.

  2. Optical clearing and multiphoton imaging of paraffin-embedded specimens

    NASA Astrophysics Data System (ADS)

    Wilson, Jesse W.; Degan, Simone; Fischer, Martin C.; Warren, Warren S.

    2013-02-01

    New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffin-embedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies.

  3. Convergent perturbation analysis of intense coherent multiphoton interactions

    NASA Technical Reports Server (NTRS)

    Gower, M. C.; Yee, T. K.; Gustafson, T. K.; Fan, B.

    1979-01-01

    Use has been made of flow graphs to deduce Feenberg perturbation expansions for radiative interactions. It is demonstrated that these expansions can in certain cases be summed to provide closed form expressions for the molecular response. In particular, it is shown that the coherent state response can be obtained by the summation of a continued fraction perturbation expansion for the harmonic oscillator. Anharmonicity in the lower levels is treated and its shown to introduce Rabi flopping identifiable with multiphoton transitions among isolated tightly coupled subsystems of levels. Relevance to laser induced multiphoton excitation and energy level shift calculations in the presence of a strong field are also discussed.

  4. Multiphoton interband excitations of quantum gases in driven optical lattices

    NASA Astrophysics Data System (ADS)

    Weinberg, M.; Ölschläger, C.; Sträter, C.; Prelle, S.; Eckardt, A.; Sengstock, K.; Simonet, J.

    2015-10-01

    We report on the observation of multiphoton interband absorption processes for quantum gases in shaken light crystals. Periodic inertial forcing, induced by a spatial motion of the lattice potential, drives multiphoton interband excitations of up to the ninth order. The occurrence of such excitation features is systematically investigated with respect to the potential depth and the driving amplitude. Ab initio calculations of resonance positions as well as numerical evaluation of their strengths exhibit good agreement with experimental data. In addition our findings could make it possible to reach novel phases of quantum matter by tailoring appropriate driving schemes.

  5. Unambiguous atomic Bell measurement assisted by multiphoton states

    NASA Astrophysics Data System (ADS)

    Torres, Juan Mauricio; Bernád, József Zsolt; Alber, Gernot

    2016-05-01

    We propose and theoretically investigate an unambiguous Bell measurement of atomic qubits assisted by multiphoton states. The atoms interact resonantly with the electromagnetic field inside two spatially separated optical cavities in a Ramsey-type interaction sequence. The qubit states are postselected by measuring the photonic states inside the resonators. We show that if one is able to project the photonic field onto two coherent states on opposite sites of phase space, an unambiguous Bell measurement can be implemented. Thus, our proposal may provide a core element for future components of quantum information technology such as a quantum repeater based on coherent multiphoton states, atomic qubits and matter-field interaction.

  6. Fibre-coupled multiphoton microscope with adaptive motion compensation

    PubMed Central

    Sherlock, Ben; Warren, Sean; Stone, James; Neil, Mark; Paterson, Carl; Knight, Jonathan; French, Paul; Dunsby, Chris

    2015-01-01

    To address the challenge of sample motion during in vivo imaging, we present a fibre-coupled multiphoton microscope with active axial motion compensation. The position of the sample surface is measured using optical coherence tomography and fed back to a piezo actuator that adjusts the axial location of the objective to compensate for sample motion. We characterise the system’s performance and demonstrate that it can compensate for axial sample velocities up to 700 µm/s. Finally we illustrate the impact of motion compensation when imaging multiphoton excited autofluorescence in ex vivo mouse skin. PMID:26137387

  7. Photonic near-field imaging in multiphoton photoemission electron microscopy

    NASA Astrophysics Data System (ADS)

    Fitzgerald, J. P. S.; Word, R. C.; Saliba, S. D.; Könenkamp, R.

    2013-05-01

    We report the observation of optical near fields in a photonic waveguide of conductive indium tin oxide (ITO) using multiphoton photoemission electron microscopy (PEEM). Nonlinear two-photon photoelectron emission is enhanced at field maxima created by interference between incident 410-nm and coherently excited guided photonic waves, providing strong phase contrast. Guided modes are observed under both transverse magnetic field (TM) and transverse electric field (TE) polarized illuminations and are consistent with classical electromagnetic theory. Implications on the role of multiphoton PEEM in optical near-field imaging are discussed.

  8. Multiphoton Coherent Manipulation in Large Spin Qubits

    NASA Astrophysics Data System (ADS)

    Chiorescu, Irinel

    2009-03-01

    Manipulation of quantum information allows certain algorithms to be performed at unparalleled speeds. Photons are an ideal choice to manipulate qubits as they interact with quantum systems in predictable ways. They are a versatile tool for manipulating, reading/coupling qubits and for encoding/transferring quantum information over long distances. Spin-based qubits have well known behavior under photon driving and can be potentially operated up to room temperature. When diluted enough to avoid uncontrolled spin-spin interactions, a variety of spin qubits show long coherence times, e.g. the nitrogen vacancies in pure diamonds (1,2), nitrogen atoms trapped in a C60 cage (3), Ho3+ and Cr5+ ions (4,5) and molecular magnets (6,7). We have used large spin Mn2+ ions (S=5/2) to realize a six level system that can be operated by means of single as well as multi-photon coherent Rabi oscillations (8). This spin system has a very small anisotropy whose effect can be tuned in-situ to turn the system into a multi-level harmonic system. This offer new ways of manipulating, reading and resetting a spin qubit. Decoherence effects are strongly reduced by the quasi-isotropic electron interaction with the crystal field and with the 55Mn nuclear spins. [0pt] 1. R. Hanson et al., Science 320, 352 (2008). [0pt] 2. M.V. Gurudev Dutt et al., Science 316, 1312 (2007). [0pt] 3. G.W. Morley et al., Phys. Rev. Lett. 98, 220501 (2007). [0pt] 4. S. Bertaina et al., Nat. Nanotech. 2, 39 (2007). [0pt] 5. S. Nellutla et al., Phys. Rev. Lett. 99, 137601 (2007). [0pt] 6. A. Ardavan et al., Phys. Rev. Lett. 98, 057201 (2007). [0pt] 7. S. Bertaina et al., Nature 453, 203,(2008). [0pt] 8. S. Bertaina et al., submitted.

  9. Quantum critical dynamics of a qubit coupled to an isotropic Lipkin-Meshkov-Glick bath

    SciTech Connect

    Quan, H. T.; Wang, Z. D.; Sun, C. P.

    2007-07-15

    We explore a dynamic signature of quantum phase transition (QPT) in an isotropic Lipkin-Meshkov-Glick (LMG) model by studying the time evolution of a central qubit coupled to it. We evaluate exactly the time-dependent purity, which can be used to measure quantum coherence, of the central qubit. It is found that distinctly different behaviors of the purity as a function of the parameter reveal clearly the QPT point in the system. It is also clarified that the present model is equivalent to an anti-Jaynes-Cummings model under certain conditions.

  10. FAST TRACK COMMUNICATION: \\ {P}\\ {T}-symmetry, Cartan decompositions, Lie triple systems and Krein space-related Clifford algebras

    NASA Astrophysics Data System (ADS)

    Günther, Uwe; Kuzhel, Sergii

    2010-10-01

    Gauged \\ {P}\\ {T} quantum mechanics (PTQM) and corresponding Krein space setups are studied. For models with constant non-Abelian gauge potentials and extended parity inversions compact and noncompact Lie group components are analyzed via Cartan decompositions. A Lie-triple structure is found and an interpretation as \\ {P}\\ {T}-symmetrically generalized Jaynes-Cummings model is possible with close relation to recently studied cavity QED setups with transmon states in multilevel artificial atoms. For models with Abelian gauge potentials a hidden Clifford algebra structure is found and used to obtain the fundamental symmetry of Krein space-related J-self-adjoint extensions for PTQM setups with ultra-localized potentials.

  11. Physics and Probability

    NASA Astrophysics Data System (ADS)

    Grandy, W. T., Jr.; Milonni, P. W.

    2004-12-01

    Preface; 1. Recollection of an independent thinker Joel A. Snow; 2. A look back: early applications of maximum entropy estimation to quantum statistical mechanics D. J. Scalapino; 3. The Jaynes-Cummings revival B. W. Shore and P. L. Knight; 4. The Jaynes-Cummings model and the one-atom-master H. Walther; 5. The Jaynes-Cummings model is alive and well P. Meystre; 6. Self-consistent radiation reaction in quantum optics - Jaynes' influence and a new example in cavity QED J. H. Eberly; 7. Enhancing the index of refraction in a nonabsorbing medium: phaseonium versus a mixture of two-level atoms M. O. Scully, T. W. Hänsch, M. Fleischhauer, C. H. Keitel and Shi-Yao Zhu; 8. Ed Jaynes' steak dinner problem II Michael D. Crisp; 9. Source theory of vacuum field effects Peter W. Milonni; 10. The natural line shape Edwin A. Power; 11. An operational approach to Schrödinger's cat L. Mandel; 12. The classical limit of an atom C. R. Stroud, Jr.; 13. Mutual radiation reaction in spontaneous emission Richard J. Cook; 14. A model of neutron star dynamics F. W. Cummings; 15. The kinematic origin of complex wave function David Hestenes; 16. On radar target identification C. Ray Smith; 17. On the difference in means G. Larry Bretthorst; 18. Bayesian analysis, model selection and prediction Arnold Zellner and Chung-ki Min; 19. Bayesian numerical analysis John Skilling; 20. Quantum statistical inference R. N. Silver; 21. Application of the maximum entropy principle to nonlinear systems far from equilibrium H. Haken; 22. Nonequilibrium statistical mechanics Baldwin Robertson; 23. A backward look to the future E. T. James; Appendix. Vita and bibliography of Edwin T. Jaynes; Index.

  12. Self-organized pattern formation in laser-induced multiphoton ionization

    NASA Astrophysics Data System (ADS)

    Buschlinger, Robert; Nolte, Stefan; Peschel, Ulf

    2014-05-01

    We use finite-difference time-domain modeling to investigate plasma generation induced by multiphoton absorption of intense laser light in dielectrics with tiny inhomogeneities. Plasma generation is found to be strongly amplified around nanometer-sized inhomogeneities as present in glasses. Each inhomogeneity acts as the seed of a plasma structure growing against the direction of light propagation. Plasma structures originating from randomly distributed inhomogeneities are found to interact strongly and to organize in regularly spaced planes oriented perpendicularly to the laser polarization. We discuss similarities between our results and nanogratings in fused silica written by laser beams with spatially homogeneous as well as radial and azimuthal polarizations.

  13. Waveguide-integrated single- and multi-photon detection at telecom wavelengths using superconducting nanowires

    SciTech Connect

    Ferrari, Simone; Kahl, Oliver; Kovalyuk, Vadim; Goltsman, Gregory N.; Korneev, Alexander; Pernice, Wolfram H. P.

    2015-04-13

    We investigate single- and multi-photon detection regimes of superconducting nanowire detectors embedded in silicon nitride nanophotonic circuits. At near-infrared wavelengths, simultaneous detection of up to three photons is observed for 120 nm wide nanowires biased far from the critical current, while narrow nanowires below 100 nm provide efficient single photon detection. A theoretical model is proposed to determine the different detection regimes and to calculate the corresponding internal quantum efficiency. The predicted saturation of the internal quantum efficiency in the single photon regime agrees well with plateau behavior observed at high bias currents.

  14. Energetics from Slow Infrared Multiphoton Dissociation of Biomolecules

    PubMed Central

    Jockusch, Rebecca A.; Paech, Kolja

    2005-01-01

    Photodissociation kinetics of the protonated pentapeptide leucine enkephalin measured using a cw CO2 laser and a Fourier-transform mass spectrometer are reported. A short induction period, corresponding to the time required to raise the internal energy of the ion population to a (dissociating) steady state, is observed. After this induction period, the dissociation data are accurately fit by first-order kinetics. A plot of the log of the unimolecular dissociation rate constant, kuni, as a function of the log of laser power is linear at low laser powers (<9 W, kuni <0.05 s−1), but tapers off at high laser power (9–33 W, kuni = 0.05–7 s−1). The entire measured dissociation curve can be accurately fit by an exponential function plus a constant. The experiment is simulated using a master equation formalism. In the model, the laser radiation is described as an energetically flat-topped distribution which is spatially uniform. This description is consistent with experimental results which indicate that ion motion within the cell averages out spatial inhomogeneities in the laser light. The model has several adjustable parameters. The effect of varying these parameters on the calculated kinetics and power dependence curves is discussed. A procedure for determining a limited range of threshold dissociation energy, Eo, which fits both the measured induction period and power dependence curves, is presented. Using this procedure, Eo of leucine enkephalin is determined to be 1.12–1.46 eV. This result is consistent with, although less precise than, values measured previously using blackbody infrared radiative dissociation. Although the blackbody dissociation results were used as a starting point to search for fits of the master equation model to experiment, these results demonstrate that it is, in principle, possible to determine a limited range of Eo from slow infrared multiphoton dissociation data alone. PMID:16467893

  15. Cavity-Assisted Spin Orbit Coupling

    NASA Astrophysics Data System (ADS)

    Zhu, Chuanzhou; Dong, Lin; Pu, Han

    We consider a single ultracold atom trapped inside a single-mode optical cavity, where a two-photon Raman process induces an effective coupling between atom's pseudo-spin and external center-of-mass (COM) motion. Without the COM motion, this system is described by the Jaynes-Cummings (JC) model. We show how the atomic COM motion dramatically modifies the predictions based on the JC model. We also investigated the situation when cavity pumping and decay are taken into account. We take a quantum Master equation approach to study this open system and again show how the cavity-induced spin-orbit coupling affects the properties of the system.

  16. Cavity-Induced Spin-Orbit Coupling in Cold Atoms

    NASA Astrophysics Data System (ADS)

    Zhu, Chuanzhou; Dong, Lin; Pu, Han

    2016-05-01

    We consider a single ultracold atom trapped inside a single-mode optical cavity, where a two-photon Raman process induces an effective coupling between atom's pseudo-spin and external center-of-mass (COM) motion. Without the COM motion, this system is described by the Jaynes-Cummings (JC) model. We show how the atomic COM motion dramatically modifies the predictions based on the JC model, and how the cavity photon field affects the properties of spin-orbit coupled system. We take a quantum Master equation approach to investigate the situation when the cavity pumping and decay are taken into account.

  17. Controlling the transmitted information of a multi-photon interacting with a single-Cooper pair box

    SciTech Connect

    Kadry, Heba Abdel-Aty, Abdel-Haleem Zakaria, Nordin; Cheong, Lee Yen

    2014-10-24

    We study a model of a multi-photon interaction of a single Cooper pair box with a cavity field. The exchange of the information using this system is studied. We quantify the fidelity of the transmitted information. The effect of the system parameters (detuning parameter, field photons, state density and mean photon number) in the fidelity of the transmitted information is investigated. We found that the fidelity of the transmitted information can be controlled using the system parameters.

  18. Comparison of objective lenses for multiphoton microscopy in turbid samples.

    PubMed

    Singh, Avtar; McMullen, Jesse D; Doris, Eli A; Zipfel, Warren R

    2015-08-01

    Optimization of illumination and detection optics is pivotal for multiphoton imaging in highly scattering tissue and the objective lens is the central component in both of these pathways. To better understand how basic lens parameters (NA, magnification, field number) affect fluorescence collection and image quality, a two-detector setup was used with a specialized sample cell to separate measurement of total excitation from epifluorescence collection. Our data corroborate earlier findings that low-mag lenses can be superior at collecting scattered photons, and we compare a set of commonly used multiphoton objective lenses in terms of their ability to collect scattered fluorescence, providing guidance for the design of multiphoton imaging systems. For example, our measurements of epi-fluorescence beam divergence in the presence of scattering reveal minimal beam broadening, indicating that often-advocated over-sized collection optics are not as advantageous as previously thought. These experiments also provide a framework for choosing objective lenses for multiphoton imaging by relating the results of our measurements to various design parameters of the objectives lenses used. PMID:26309771

  19. The influence of phase damping on a two-level atom in the presence of the classical laser field

    NASA Astrophysics Data System (ADS)

    Sebawe Abdalla, M.; Obada, A.-S. F.; Khalil, E. M.; Ali, S. I.

    2013-11-01

    In this paper we consider the influence of phase damping on the Jaynes-Cummings model (JCM) in the presence of the classical laser field. It is shown that for the temporal evolution of the atomic inversion a detuning parameter plays a role in delaying the effect of the damping. Our consideration is also extended to discuss the atomic Wehrl entropy and entropy squeezing. For the case of the marginal distribution, it is noted that the damping factor plays a considerable role in reducing the number of the fluctuations in the function behavior. On the other hand the damping factor removes the phenomenon of squeezing from both quadratures of the entropy squeezing.

  20. Vacuum Rabi splitting effect in nanomechanical QED system with nonlinear resonator

    NASA Astrophysics Data System (ADS)

    Zhao, MingYue; Gao, YiBo

    2016-08-01

    Considering the intrinsic nonlinearity in a nanomechanical resonator coupled to a charge qubit, vacuum Rabi splitting effect is studied in a nanomechanical QED (qubit-resonator) system. A driven nonlinear Jaynes-Cummings model describes the dynamics of this qubit-resonator system. Using quantum regression theorem and master equation approach, we have calculated the two-time correlation spectrum analytically. In the weak driving limit, these analytical results clarify the influence of the driving strength and nonlinearity parameter on the correlation spectrum. Also, numerical calculations confirm these analytical results.

  1. Evolution of entanglement under echo dynamics

    SciTech Connect

    Prosen, Tomaz; Znidaric, Marko; Seligman, Thomas H.

    2003-04-01

    Echo dynamics and fidelity are often used to discuss stability in quantum-information processing and quantum chaos. Yet fidelity yields no information about entanglement, the characteristic property of quantum mechanics. We study the evolution of entanglement in echo dynamics. We find qualitatively different behavior between integrable and chaotic systems on one hand and between random and coherent initial states for integrable systems on the other. For the latter the evolution of entanglement is given by a classical time scale. Analytic results are illustrated numerically in a Jaynes-Cummings model.

  2. Transient Sub-Poissonian Distribution for Single-Mode Lasers

    NASA Technical Reports Server (NTRS)

    Zang, J. Y.; Gu, Q.; Tian, L. K.

    1996-01-01

    In this paper, the transient photon statistics for single-mode lasers is investigated by making use of the theory of quantum electrodynamics. By taking into account of the transitive time l,we obtain the master equation for Jaynes-Cummings model. The relation between the Mandel factor and the time is obtained by directly solving the master equation. The result shows that a transient phenomenon from the transient super-Poissonian distribution to the transient sub-Poissonian distribution occurs for single-mode lasers. In addition, the influences of the thermal light field and the cavity loss on the transient sub-Poissonian distribution are also studied.

  3. Real and imaginary negative binomial states

    NASA Astrophysics Data System (ADS)

    Liao, Jing; Wang, Xiaoguang; Wu, Ling-An; Pan, Shao-Hua

    2001-10-01

    The real and imaginary negative binomial states formed by a superposition of the negative binomial states are introduced. The sub-Poissonian statistics, Wigner function and squeezing properties of the real and imaginary states are studied in detail. The oscillatory character of the photon distribution due to the quantum interference between the two components is shown. Moreover, we find that these states are real and imaginary nonlinear Schrödinger cat states and give the corresponding ladder operator formalisms. We also discuss how to generate these general real quantum superposition states based on the intensity-dependent Jaynes-Cummings model.

  4. Using a hybrid system (Cooper pair box plus nanomechanical resonator) in the presence of Kerr nonlinearities and losses to control the entropy of the subsystems

    NASA Astrophysics Data System (ADS)

    Valverde, C.; Castro, A. N.; Baseia, B.

    2016-05-01

    We consider the Jaynes-Cummings model to describe the interaction of a Cooper pair box and a nanomechanical resonator in the presence of a Kerr medium and losses. The evolution of the entropy of both subsystems and the Cooper pair box population inversion were calculated numerically. It was found that population inversion and entropy increase when the frequency of the nanoresonator is time-dependent, even in the presence of losses; the effect is very sensitive to detuning and disappears in resonant regime. We also compare effects of the losses on each subsystem.

  5. Deep-tissue multiphoton fluorescence lifetime microscopy for intravital imaging of protein-protein interactions

    NASA Astrophysics Data System (ADS)

    Fruhwirth, G. O.; Matthews, D. R.; Brock, A.; Keppler, M.; Vojnovic, B.; Ng, T.; Ameer-Beg, S.

    2009-02-01

    Fluorescent lifetime imaging microscopy (FLIM) has proven to be a valuable tool in beating the Rayleigh criterion for light microscopy by measuring Förster resonance energy transfer (FRET) between two fluorophores. Applying multiphoton FLIM, we previously showed in a human breast cancer cell line that recycling of a membrane receptorgreen fluorescent protein fusion is enhanced concomitantly with the formation of a receptor:protein kinase C α complex in the endosomal compartment. We have extended this established technique to probe direct protein-protein interactions also in vivo. Therefore, we used various expressible fluorescent tags fused to membrane receptor molecules in order to generate stable two-colour breast carcinoma cell lines via controlled retroviral infection. We used these cell lines for establishing a xenograft tumour model in immune-compromised Nude mice. Using this animal model in conjunction with scanning Ti:Sapphire laser-based two-photon excitation, we established deep-tissue multiphoton FLIM in vivo. For the first time, this novel technique enables us to directly assess donor fluorescence lifetime changes in vivo and we show the application of this method for intravital imaging of direct protein-protein interactions.

  6. Multiphoton ionisation and dissociation of NO 2 by 50 fs laser pulses

    NASA Astrophysics Data System (ADS)

    Singhal, R. P.; Kilic, H. S.; Ledingham, K. W. D.; Kosmidis, C.; McCanny, T.; Langley, A. J.; Shaikh, W.

    1996-04-01

    Multiphoton ionisation and dissociation of NO 2 has been studied experimentally at 375 nm for laser pulse widths of 10 ns and 50 fs. The parent NO 2 ion peak is not seen in the ns data. In all spectra, the main peak observed is due to the ionisation of the NO molecule which results from the dissociation of excited NO 2 formed after absorbing a 375 nm photon. The intensity dependencies of both NO and NO 2 ion peaks have also been measured. The data has been analysed within the context of a rate equation model using published cross-sections and dissociation rates except for the two-photon ionisation cross-section for NO 2 which was chosen to reproduce the NO 2/NO ion signal ratios at 50 fs. The rate equation model provides a good description of the complete set of data. Indirectly, it may be concluded that coherence effects do not play an important role in the multiphoton excitation/ionisation of NO 2. The data also rules out the importance of above-ionisation dissociation in NO 2 — a conclusion which is consistent with previous data at 496 and 248 nm for laser pulse widths ⩾ 300 fs.

  7. Multiphoton absorption is probably not the primary threshold damage mechanism for femtosecond laser pulse exposures in the retinal pigment epithelium

    NASA Astrophysics Data System (ADS)

    Glickman, Randolph D.; Johnson, Thomas E.

    2004-07-01

    Laser induced breakdown has the lowest energy threshold in the femtosecond domain, and is responsible for production of threshold ocular lesions. It has been proposed that multiphoton absorption may also contribute to ultrashort-pulse tissue damage, based on the observation that 33 fs, 810 nm pulse laser exposures caused more DNA breakage in cultured, primary RPE cells, compared to CW laser exposures delivering the same average power. Subsequent studies, demonstrating two-photon excitation of fluorescence in isolated RPE melanosomes, appeared to support the role of multiphoton absorption, but mainly at suprathreshold irradiance. Additional experiments have not found a consistent difference in the DNA strand breakage produced by ultrashort and CW threshold exposures. DNA damage appears to be dependent on the amount of melanin pigmentation in the cells, rather than the pulsewidth of the laser; current studies have found that, at threshold, CW and ultrashort pulse laser exposures produce almost identical amounts of DNA breakage. A theoretical analysis suggest that the number of photons delivered to the RPE melanosome during a single 33-fsec pulse at the ED50 irradiance is insufficient to produce multiphoton excitation. This result appears to exclude the melanosome as a locus for two- or three-photon excitation; however, a structure with a larger effective absorption cross-section than the melanosome may interact with the laser pulses. One possibility is that the nuclear chromatin acts as a unit absorber of photons resulting in DNA damage, but this does not explain the near equivalence of ultrashort and CW exposures in the comet assay model. This equivalence indicated that multiphoton absorption is not a major contributor to the ultrashort pulse laser damage threshold in the near infrared.

  8. Full analysis of multi-photon pair effects in spontaneous parametric down conversion based photonic quantum information processing

    NASA Astrophysics Data System (ADS)

    Takeoka, Masahiro; Jin, Rui-Bo; Sasaki, Masahide

    2015-04-01

    In spontaneous parametric down conversion (SPDC) based quantum information processing (QIP) experiments, there is a tradeoff between the coincidence count rates (i.e. the pumping power of the SPDC), which limits the rate of the protocol, and the visibility of the quantum interference, which limits the quality of the protocol. This tradeoff is mainly caused by the multi-photon pair emissions from the SPDCs. In theory, the problem is how to model the experiments without truncating these multi-photon emissions while including practical imperfections. In this paper, we establish a method to theoretically simulate SPDC-based QIPs which fully incorporates the effect of multi-photon emissions and various practical imperfections. The key ingredient in our method is the application of the characteristic function formalism which has been used in continuous variable QIPs. We apply our method to three examples, the Hong-Ou-Mandel interference and the Einstein-Podolsky-Rosen interference experiments, and the concatenated entanglement swapping protocol. For the first two examples, we show that our theoretical results quantitatively agree with the recent experimental results. Also we provide the closed expressions for these interference visibilities with the full multi-photon components and various imperfections. For the last example, we provide the general theoretical form of the concatenated entanglement swapping protocol in our method and show the numerical results up to five concatenations. Our method requires only a small computational resource (a few minutes by a commercially available computer), which was not possible in the previous theoretical approach. Our method will have applications in a wide range of SPDC-based QIP protocols with high accuracy and a reasonable computational resource.

  9. Video-rate resonant scanning multiphoton microscopy: An emerging technique for intravital imaging of the tumor microenvironment.

    PubMed

    Kirkpatrick, Nathaniel D; Chung, Euiheon; Cook, Daniel C; Han, Xiaoxing; Gruionu, Gabriel; Liao, Shan; Munn, Lance L; Padera, Timothy P; Fukumura, Dai; Jain, Rakesh K

    2012-01-01

    The abnormal tumor microenvironment fuels tumor progression, metastasis, immune suppression, and treatment resistance. Over last several decades, developments in and applications of intravital microscopy have provided unprecedented insights into the dynamics of the tumor microenvironment. In particular, intravital multiphoton microscopy has revealed the abnormal structure and function of tumor-associated blood and lymphatic vessels, the role of aberrant tumor matrix in drug delivery, invasion and metastasis of tumor cells, the dynamics of immune cell trafficking to and within tumors, and gene expression in tumors. However, traditional multiphoton microscopy suffers from inherently slow imaging rates-only a few frames per second, thus unable to capture more rapid events such as blood flow, lymphatic flow, and cell movement within vessels. Here, we report the development and implementation of a video-rate multiphoton microscope (VR-MPLSM) based on resonant galvanometer mirror scanning that is capable of recording at 30 frames per second and acquiring intravital multispectral images. We show that the design of the system can be readily implemented and is adaptable to various experimental models. As examples, we demonstrate the utility of the system to directly measure flow within tumors, capture metastatic cancer cells moving within the brain vasculature and cells in lymphatic vessels, and image acute responses to changes in a vascular network. VR-MPLSM thus has the potential to further advance intravital imaging and provide new insight into the biology of the tumor microenvironment. PMID:24353926

  10. Hybrid multiphoton volumetric functional imaging of large-scale bioengineered neuronal networks

    NASA Astrophysics Data System (ADS)

    Dana, Hod; Marom, Anat; Paluch, Shir; Dvorkin, Roman; Brosh, Inbar; Shoham, Shy

    2014-06-01

    Planar neural networks and interfaces serve as versatile in vitro models of central nervous system physiology, but adaptations of related methods to three dimensions (3D) have met with limited success. Here, we demonstrate for the first time volumetric functional imaging in a bioengineered neural tissue growing in a transparent hydrogel with cortical cellular and synaptic densities, by introducing complementary new developments in nonlinear microscopy and neural tissue engineering. Our system uses a novel hybrid multiphoton microscope design combining a 3D scanning-line temporal-focusing subsystem and a conventional laser-scanning multiphoton microscope to provide functional and structural volumetric imaging capabilities: dense microscopic 3D sampling at tens of volumes per second of structures with mm-scale dimensions containing a network of over 1,000 developing cells with complex spontaneous activity patterns. These developments open new opportunities for large-scale neuronal interfacing and for applications of 3D engineered networks ranging from basic neuroscience to the screening of neuroactive substances.

  11. Comparison Study of Atomic and Molecular Single Ionization in the Multiphoton Ionization Regime

    SciTech Connect

    Wu Jian; Zeng Heping; Guo Chunlei

    2006-06-23

    In this Letter, we report, for the first time in the multiphoton ionization regime, a comparison study of single-electron ionization of diatomic molecules versus rare gas atoms with virtually the same ionization potentials. In comparing N{sub 2}{sup +} to Ar{sup +}, a higher ion signal is seen in N{sub 2}{sup +} compared to Ar{sup +} for linear polarization but the difference vanishes in circularly polarized light. In comparing O{sub 2}{sup +} to Xe{sup +}, we observe a suppression in O{sub 2}{sup +} compared to Xe{sup +} for both linear and circular polarization but this suppression exhibits an intensity dependence; i.e., there is little suppression for O{sub 2}{sup +} at the lowest intensity range, but the suppression becomes increasingly stronger as the laser intensity increases. The multielectron screening model is used to discuss possible mechanisms of this intensity dependent suppression in O{sub 2}{sup +} in the multiphoton ionization regime.

  12. In vivo multiphoton NADH fluorescence reveals depth-dependent keratinocyte metabolism in human skin.

    PubMed

    Balu, Mihaela; Mazhar, Amaan; Hayakawa, Carole K; Mittal, Richa; Krasieva, Tatiana B; König, Karsten; Venugopalan, Vasan; Tromberg, Bruce J

    2013-01-01

    We employ a clinical multiphoton microscope to monitor in vivo and noninvasively the changes in reduced nicotinamide adenine dinucleotide (NADH) fluorescence of human epidermal cells during arterial occlusion. We correlate these results with measurements of tissue oxy- and deoxyhemoglobin concentration during oxygen deprivation using spatial frequency domain imaging. During arterial occlusion, a decrease in oxyhemoglobin corresponds to an increase in NADH fluorescence in the basal epidermal cells, implying a reduction in basal cell oxidative phosphorylation. The ischemia-induced oxygen deprivation is associated with a strong increase in NADH fluorescence of keratinocytes in layers close to the stratum basale, whereas keratinocytes from epidermal layers closer to the skin surface are not affected. Spatial frequency domain imaging optical property measurements, combined with a multilayer Monte Carlo-based radiative transport model of multiphoton microscopy signal collection in skin, establish that localized tissue optical property changes during occlusion do not impact the observed NADH signal increase. This outcome supports the hypothesis that the vascular contribution to the basal layer oxygen supply is significant and these cells engage in oxidative metabolism. Keratinocytes in the more superficial stratum granulosum are either supplied by atmospheric oxygen or are functionally anaerobic. Based on combined hemodynamic and two-photon excited fluorescence data, the oxygen consumption rate in the stratum basale is estimated to be ∼0.035 μmoles/10(6) cells/h. PMID:23332078

  13. In Vivo Multiphoton NADH Fluorescence Reveals Depth-Dependent Keratinocyte Metabolism in Human Skin

    PubMed Central

    Balu, Mihaela; Mazhar, Amaan; Hayakawa, Carole K.; Mittal, Richa; Krasieva, Tatiana B.; König, Karsten; Venugopalan, Vasan; Tromberg, Bruce J.

    2013-01-01

    We employ a clinical multiphoton microscope to monitor in vivo and noninvasively the changes in reduced nicotinamide adenine dinucleotide (NADH) fluorescence of human epidermal cells during arterial occlusion. We correlate these results with measurements of tissue oxy- and deoxyhemoglobin concentration during oxygen deprivation using spatial frequency domain imaging. During arterial occlusion, a decrease in oxyhemoglobin corresponds to an increase in NADH fluorescence in the basal epidermal cells, implying a reduction in basal cell oxidative phosphorylation. The ischemia-induced oxygen deprivation is associated with a strong increase in NADH fluorescence of keratinocytes in layers close to the stratum basale, whereas keratinocytes from epidermal layers closer to the skin surface are not affected. Spatial frequency domain imaging optical property measurements, combined with a multilayer Monte Carlo-based radiative transport model of multiphoton microscopy signal collection in skin, establish that localized tissue optical property changes during occlusion do not impact the observed NADH signal increase. This outcome supports the hypothesis that the vascular contribution to the basal layer oxygen supply is significant and these cells engage in oxidative metabolism. Keratinocytes in the more superficial stratum granulosum are either supplied by atmospheric oxygen or are functionally anaerobic. Based on combined hemodynamic and two-photon excited fluorescence data, the oxygen consumption rate in the stratum basale is estimated to be ∼0.035 μmoles/106 cells/h. PMID:23332078

  14. Chronic imaging of amyloid plaques in the live mouse brain using multiphoton microscopy

    NASA Astrophysics Data System (ADS)

    Bacskai, Brian J.; Kajdasz, Stephen T.; Christie, R. H.; Zipfel, Warren R.; Williams, Rebecca M.; Kasischke, Karl A.; Webb, Watt W.; Hyman, B. T.

    2001-04-01

    Transgenic mice expressing the human Amyloid Precursor Protein (APP) develop amyloid plaques as they age. These plaques resemble those found in the human disease. Multiphoton laser scanning microscopy combined with a novel surgical approach was used to measure amyloid plaque dynamics chronically in the cortex of living transgenic mice. Thioflavine S (thioS) was used as a fluorescent marker of amyloid deposits. Multiphoton excitation allowed visualization of amyloid plaques up to 200 micrometers deep into the brain. The surgical site could be imaged repeatedly without overt damage to the tissue, and individual plaques within this volume could be reliably identified over periods of several days to several months. On average, plaque sizes remained constant over time, supporting a model of rapid deposition, followed by relative stability. Alternative reporters for in vivo histology include thiazine red, and FITC-labeled amyloid-(Beta) peptide. We also present examples of multi-color imaging using Hoechst dyes and FITC-labeled tomato lectin. These approaches allow us to observe cell nuclei or microglia simultaneously with amyloid-(Beta) deposits in vivo. Chronic imaging of a variety of reporters in these transgenic mice should provide insight into the dynamics of amyloid-(Beta) activity in the brain.

  15. Photon-momentum transfer in multiphoton ionization and in time-resolved holography with photoelectrons

    NASA Astrophysics Data System (ADS)

    Chelkowski, Szczepan; Bandrauk, André D.; Corkum, Paul B.

    2015-11-01

    In most models and theoretical calculations describing multiphoton ionization by infrared light, the dipole approximation is used. This is equivalent to setting the very small photon momentum to zero. Using numerical solutions of the two-dimensional (2-D) time-dependent Schrödinger equation for one electron (H-like) systems, we show that, for linear polarization, the radiation pressure on photoelectrons is very sensitive to the details of the ionization mechanism. The directly ionized photoelectrons, those that never recollide with the parent ion, are driven in the direction of the laser photon momentum, whereas a fraction of slower photoelectrons are pushed in the opposite direction, leading to the counterintuitive shifts observed in recent experiments [Phys. Rev. Lett. 113, 243001 (2014), 10.1103/PhysRevLett.113.243001]. This complex response is due to the interplay between the Lorentz force and the Coulomb attraction from the ion. On average, however, the photoelectron momentum is in the direction of the photon momentum as in the case of circular polarization. The influence of the photon momentum is shown to be discernible in the holographic patterns of time-resolved atomic and molecular holography with photoelectrons, thus suggesting a new research subject in multiphoton ionization.

  16. Monitoring photoaging by use of multiphoton fluorescence and second harmonic generation microscopy

    NASA Astrophysics Data System (ADS)

    Lin, Sung-Jan; Jee, Shiou-Hwa; Chan, Jung-Yi; Wu, Ruei-Jr; Lo, Wen; Tan, Hsin-Yuan; Lin, Wei-Chou; Chen, Jau-Shiuh; Young, Tai-Horng; Hsu, Chih-Jung; Dong, Chen-Yuan

    2006-02-01

    It is a field of great interest to develop therapies to rejuvenate photoaged skin. However, the treatment response can not be ideally determined due to lack of a reliable non-invasive method to quantify photoaging. In this study, the photoaging process of skin is investigated by use of a multiphoton fluorescence and second harmonic generation microscopy. We obtain the autofluorescence and second harmonic generation images of superficial dermis from facial skin of individuals of different ages. The results show that autofluorescence signals increase with age while second harmonic generation signals decrease with age. The results are consistent with the histological findings in which collagen is progressively replaced by elastic fibers. In the case of severe photoaging, solar elastosis can be clearly demonstrated by the presence of thick curvy autofluorescent materials in the superficial dermis. We propose a second harmonic generation to autofluorescence aging index of dermis to quantify the photoaging changes. This index is shown to be a good indicator of photoaging. Our results suggest that multiphoton fluorescence and second harmonic generation microscopy can be developed into a non-invasive imaging modelity for the clinical evaluation of photoaging.

  17. Multiphoton microscopy and microspectroscopy for diagnostics of inflammatory and neoplastic lung

    NASA Astrophysics Data System (ADS)

    Pavlova, Ina; Hume, Kelly R.; Yazinski, Stephanie A.; Flanders, James; Southard, Teresa L.; Weiss, Robert S.; Webb, Watt W.

    2012-03-01

    Limitations of current medical procedures for detecting early lung cancers inspire the need for new diagnostic imaging modalities for the direct microscopic visualization of lung nodules. Multiphoton microscopy (MPM) provides for subcellular resolution imaging of intrinsic fluorescence from unprocessed tissue with minimal optical attenuation and photodamage. We demonstrate that MPM detects morphological and spectral features of lung tissue and differentiates between normal, inflammatory and neoplastic lung. Ex vivo MPM imaging of intrinsic two-photon excited fluorescence was performed on mouse and canine neoplastic, inflammatory and tumor-free lung sites. Results showed that MPM detected microanatomical differences between tumor-free and neoplastic lung tissue similar to standard histopathology but without the need for tissue processing. Furthermore, inflammatory sites displayed a distinct red-shifted fluorescence compared to neoplasms in both mouse and canine lung, and adenocarcinomas displayed a less pronounced fluorescence emission in the 500 to 550 nm region compared to adenomas in mouse models of lung cancer. These spectral distinctions were also confirmed by two-photon excited fluorescence microspectroscopy. We demonstrate the feasibility of applying MPM imaging of intrinsic fluorescence for the differentiation of lung neoplasms, inflammatory and tumor-free lung, which motivates the application of multiphoton endoscopy for the in situ imaging of lung nodules.

  18. Setup and use of a two-laser multiphoton microscope for multichannel intravital fluorescence imaging

    PubMed Central

    Entenberg, David; Wyckoff, Jeffrey; Gligorijevic, Bojana; Roussos, Evanthia T; Verkhusha, Vladislav V; Pollard, Jeffrey W; Condeelis, John

    2014-01-01

    Characterizing biological mechanisms dependent upon the interaction of many cell types in vivo requires both multiphoton microscope systems capable of expanding the number and types of fluorophores that can be imaged simultaneously while removing the wavelength and tunability restrictions of existing systems, and enhanced software for extracting critical cellular parameters from voluminous 4D data sets. We present a procedure for constructing a two-laser multiphoton microscope that extends the wavelength range of excitation light, expands the number of simultaneously usable fluorophores and markedly increases signal to noise via ‘over-clocking’ of detection. We also utilize a custom-written software plug-in that simplifies the quantitative tracking and analysis of 4D intravital image data. We begin by describing the optics, hardware, electronics and software required, and finally the use of the plug-in for analysis. We demonstrate the use of the setup and plug-in by presenting data collected via intravital imaging of a mouse model of breast cancer. The procedure may be completed in ~24 h. PMID:21959234

  19. Multiphoton dynamics of qutrits in the ultrastrong coupling regime with a quantized photonic field

    NASA Astrophysics Data System (ADS)

    Avetissian, H. K.; Avetissian, A. K.; Mkrtchian, G. F.; Kibis, O. V.

    2015-12-01

    Multiphoton resonant excitation of a three-state quantum system (a qutrit) with a single-mode photonic field is considered in the ultrastrong coupling regime, when the qutrit-photonic field coupling rate is comparable to appreciable fractions of the photon frequency. For ultrastrong couplings, the obtained solutions of the Schrödinger equation that reveal multiphoton Rabi oscillations in qutrits with the interference effects leading to the collapse and revival of atomic excitation probabilities at the direct multiphoton resonant transitions.

  20. Characteristics of subgingival calculus detection by multiphoton fluorescence microscopy

    NASA Astrophysics Data System (ADS)

    Tung, Oi-Hong; Lee, Shyh-Yuan; Lai, Yu-Lin; Chen, How-Foo

    2011-06-01

    Subgingival calculus has been recognized as a major cause of periodontitis, which is one of the main chronic infectious diseases of oral cavities and a principal cause of tooth loss in humans. Bacteria deposited in subgingival calculus or plaque cause gingival inflammation, function deterioration, and then periodontitis. However, subgingival calculus within the periodontal pocket is a complicated and potentially delicate structure to be detected with current dental armamentaria, namely dental x-rays and dental probes. Consequently, complete removal of subgingival calculus remains a challenge to periodontal therapies. In this study, the detection of subgingival calculus employing a multiphoton autofluorescence imaging method was characterized in comparison with a one-photon confocal fluorescence imaging technique. Feasibility of such a system was studied based on fluorescence response of gingiva, healthy teeth, and calculus with and without gingiva covered. The multiphoton fluorescence technology perceived the tissue-covered subgingival calculus that cannot be observed by the one-photon confocal fluorescence method.

  1. Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging

    NASA Astrophysics Data System (ADS)

    Wang, Taejun; Jang, Won Hyuk; Lee, Seunghun; Yoon, Calvin J.; Lee, Jun Ho; Kim, Bumju; Hwang, Sekyu; Hong, Chun-Pyo; Yoon, Yeoreum; Lee, Gilgu; Le, Viet-Hoan; Bok, Seoyeon; Ahn, G.-One; Lee, Jaewook; Gho, Yong Song; Chung, Euiheon; Kim, Sungjee; Jang, Myoung Ho; Myung, Seung-Jae; Kim, Myoung Joon; So, Peter T. C.; Kim, Ki Hean

    2016-06-01

    Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies. However, MPM application to human tissues is limited by weak endogenous fluorescence in tissue and cytotoxicity of exogenous probes. Herein, we describe the applications of moxifloxacin, an FDA-approved antibiotic, as a cell-labeling agent for MPM. Moxifloxacin has bright intrinsic multiphoton fluorescence, good tissue penetration and high intracellular concentration. MPM with moxifloxacin was demonstrated in various cell lines, and animal tissues of cornea, skin, small intestine and bladder. Clinical application is promising since imaging based on moxifloxacin labeling could be 10 times faster than imaging based on endogenous fluorescence.

  2. Hybrid label-free multiphoton and optoacoustic microscopy (MPOM)

    NASA Astrophysics Data System (ADS)

    Soliman, Dominik; Tserevelakis, George J.; Omar, Murad; Ntziachristos, Vasilis

    2015-07-01

    Many biological applications require a simultaneous observation of different anatomical features. However, unless potentially harmful staining of the specimens is employed, individual microscopy techniques do generally not provide multi-contrast capabilities. We present a hybrid microscope integrating optoacoustic microscopy and multiphoton microscopy, including second-harmonic generation, into a single device. This combined multiphoton and optoacoustic microscope (MPOM) offers visualization of a broad range of structures by employing different contrast mechanisms and at the same time enables pure label-free imaging of biological systems. We investigate the relative performance of the two microscopy modalities and demonstrate their multi-contrast abilities through the label-free imaging of a zebrafish larva ex vivo, simultaneously visualizing muscles and pigments. This hybrid microscopy application bears great potential for developmental biology studies, enabling more comprehensive information to be obtained from biological specimens without the necessity of staining.

  3. The role of resonances in strong-field multiphoton processes

    SciTech Connect

    Perry, M.D.; Kulander, K.C.

    1990-10-01

    Resonantly-enhanced multiphoton ionization (REMPI) has been the subject of extensive experimental and theoretical study since the invention of the laser. Until recently, the overwhelming majority of REMPI research have been conducted at intensities less than 10{sup 12} W/cm{sup 2}. At these intensities, the strength of the applied field remains less than one percent of the atomic Coulomb field experienced by the outer electrons in a typical noble gas atom. In this regime, treatment of the applied field as a weak perturbation on the atomic system yields excellent agreement with experiment. Here, we investigate the role of resonances in multiphoton ionization at much higher intensities, specifically, we examine the behavior and influence of resonances as the strength of the applied field becomes a significant fraction of the atomic field. 33 refs., 7 figs., 2 tabs.

  4. Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging

    PubMed Central

    Wang, Taejun; Jang, Won Hyuk; Lee, Seunghun; Yoon, Calvin J.; Lee, Jun Ho; Kim, Bumju; Hwang, Sekyu; Hong, Chun-Pyo; Yoon, Yeoreum; Lee, Gilgu; Le, Viet-Hoan; Bok, Seoyeon; Ahn, G-One; Lee, Jaewook; Gho, Yong Song; Chung, Euiheon; Kim, Sungjee; Jang, Myoung Ho; Myung, Seung-Jae; Kim, Myoung Joon; So, Peter T. C.; Kim, Ki Hean

    2016-01-01

    Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies. However, MPM application to human tissues is limited by weak endogenous fluorescence in tissue and cytotoxicity of exogenous probes. Herein, we describe the applications of moxifloxacin, an FDA-approved antibiotic, as a cell-labeling agent for MPM. Moxifloxacin has bright intrinsic multiphoton fluorescence, good tissue penetration and high intracellular concentration. MPM with moxifloxacin was demonstrated in various cell lines, and animal tissues of cornea, skin, small intestine and bladder. Clinical application is promising since imaging based on moxifloxacin labeling could be 10 times faster than imaging based on endogenous fluorescence. PMID:27283889

  5. Nanoparticle metrology in sol-gels using multiphoton excited fluorescence

    NASA Astrophysics Data System (ADS)

    Karolin, J.; Geddes, C. D.; Wynne, K.; Birch, D. J. S.

    2002-01-01

    We have developed a method of measuring the growth of nanoparticles during sol-gel glass formation based on labelling the particle with a fluorescent dye and determining the multiphoton excited decay of fluorescence anisotropy due to Brownian rotation. Multiphoton excitation is shown to give a higher dynamic range of measurement than one-photon excitation. We illustrate the sub-nanometre resolution and stability of our approach by detecting a 0.8-1.1 nm silica particle hydrodynamic mean radius increase in a tetramethylorthosilicate sol at pH 2.3 labelled with rhodamine 6G and observed over ≈4 weeks and also with a stable silica colloid of radius 6 nm, pH 8.9, labelled with a 6-methoxyquinoline-type dye.

  6. Multiphoton Imaging of Ultrasound Bioeffects in the Murine Brain

    NASA Astrophysics Data System (ADS)

    Raymond, Scott; Skoch, Jesse; Bacskai, Brian; Hynynen, Kullervo

    2006-05-01

    The purpose of this study was to demonstrate the feasibility of multiphoton imaging in the murine brain during exposure to ultrasound. Our experimental setup coupled ultrasound through the ventral surface of the mouse while allowing imaging through a cranial window from the dorsal surface. Field attenuation was estimated by scanning the field after insertion of a freshly sacrificed mouse; beam profile and peak position were preserved, suggesting adequate targeting for imaging experiments. C57 mice were imaged with a Biorad multiphoton microscope while being exposed to ultrasound (f = 1.029 MHz, peak pressure ˜ 200 kPa, average power ˜ 0.18 W) with IV injection of Optison. We observed strong vasoconstriction coincident with US and Optison, as well as permeabilization of the blood-brain barrier.

  7. Moxifloxacin: Clinically compatible contrast agent for multiphoton imaging.

    PubMed

    Wang, Taejun; Jang, Won Hyuk; Lee, Seunghun; Yoon, Calvin J; Lee, Jun Ho; Kim, Bumju; Hwang, Sekyu; Hong, Chun-Pyo; Yoon, Yeoreum; Lee, Gilgu; Le, Viet-Hoan; Bok, Seoyeon; Ahn, G-One; Lee, Jaewook; Gho, Yong Song; Chung, Euiheon; Kim, Sungjee; Jang, Myoung Ho; Myung, Seung-Jae; Kim, Myoung Joon; So, Peter T C; Kim, Ki Hean

    2016-01-01

    Multiphoton microscopy (MPM) is a nonlinear fluorescence microscopic technique widely used for cellular imaging of thick tissues and live animals in biological studies. However, MPM application to human tissues is limited by weak endogenous fluorescence in tissue and cytotoxicity of exogenous probes. Herein, we describe the applications of moxifloxacin, an FDA-approved antibiotic, as a cell-labeling agent for MPM. Moxifloxacin has bright intrinsic multiphoton fluorescence, good tissue penetration and high intracellular concentration. MPM with moxifloxacin was demonstrated in various cell lines, and animal tissues of cornea, skin, small intestine and bladder. Clinical application is promising since imaging based on moxifloxacin labeling could be 10 times faster than imaging based on endogenous fluorescence. PMID:27283889

  8. Experimental Resonance Enhanced Multiphoton Ionization (REMPI) studies of small molecules

    NASA Technical Reports Server (NTRS)

    Dehmer, J. L.; Dehmer, P. M.; Pratt, S. T.; Ohalloran, M. A.; Tomkins, F. S.

    1987-01-01

    Resonance enhanced multiphoton ionization (REMPI) utilizes tunable dye lasers to ionize an atom or molecule by first preparing an excited state by multiphoton absorption and then ionizing that state before it can decay. This process is highly selective with respect to both the initial and resonant intermediate states of the target, and it can be extremely sensitive. In addition, the products of the REMPI process can be detected as needed by analyzing the resulting electrons, ions, fluorescence, or by additional REMPI. This points to a number of exciting opportunities for both basic and applied science. On the applied side, REMPI has great potential as an ultrasensitive, highly selective detector for trace, reactive, or transient species. On the basic side, REMPI affords an unprecedented means of exploring excited state physics and chemistry at the quantum-state-specific level. An overview of current studies of excited molecular states is given to illustrate the principles and prospects of REMPI.

  9. Does Infrared Multiphoton Dissociation of Vinyl Chloride Yield Cold Vinylidene?

    PubMed

    Fernando, Ravin; Qu, Chen; Bowman, Joel M; Field, Robert W; Suits, Arthur G

    2015-07-01

    Velocity map imaging of the infrared multiphoton dissociation of vinyl chloride shows the formation of HCl in rotational levels below J = 10 that are associated with the three-center elimination pathway. The total translational energy release is observed to peak at 3-5 kcal/mol, which is consistent with the low reverse barrier predicted for the formation of HCl with vinylidene coproducts. Direct dynamics trajectory studies from the three-center transition state reproduce the observed distributions and show that the associated vinylidene is formed with only modest rotational excitation, precluding Coriolis-induced mixing among the excited vibrational levels of acetylene that would lead to distribution of vinylidene character into many vibrationally mixed acetylene vibrational levels. The results suggest that infrared multiphoton dissociation of vinyl chloride is an efficient route to synthesis of stable, cold vinylidene. PMID:26266719

  10. Waveguide characterization with multi-photon photoemission electron microscopy

    NASA Astrophysics Data System (ADS)

    Fitzgerald, J. P. S.; Word, Robert C.; Saliba, Sebastian; Koenenkamp, Rolf

    2012-10-01

    Multi-photon photoemission electron microscopy (PEEM) images surface interactions of visible light with matter, showing electromagnetic (EM) waves that propagate at or near the surface. Images are interferometric, showing where incident and surface waves are in-phase (bright) and out-of-phase (dark), with strong contrast between regions of high and low rates of photoelectron emission. Interferogram analysis can determine the amplitude, wavelength, phase evolution, and propagation decay length of the surface waves. Most multi-photon PEEM studies focus on surface plasmon polaritons. We show that this technique can also be applied to conducting thin-film waveguides, measuring the properties of confined EM waves in a two-mode slab waveguide made of indium tin oxide on glass, which are consistent with waveguide theory. This research was funded by the US Department of Energy Basic Science Office under contract DE-FG02-10ER46406.