76 FR 10626 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-25

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-019)] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

78 FR 15378 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2013-03-11

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (13-022)] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

78 FR 56246 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2013-09-12

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 13-113] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

77 FR 53919 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2012-09-04

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 12-071] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

75 FR 80851 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2010-12-23

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (10-169)] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

77 FR 22807 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2012-04-17

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-029] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

76 FR 14433 - NASA Advisory Council; Science Committee; Heliophysics Subcommittee; Meeting

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2011-03-16

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-023)] NASA Advisory Council; Science... amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Heliophysics Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science Committee of the NAC...

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2010-08-31

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-096)] NASA Advisory Council; Science... amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Heliophysics Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science Committee of the NAC...

76 FR 28470 - NASA Advisory Council; Science Committee; Heliophysics Subcommittee; Meeting

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2011-05-17

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-047)] NASA Advisory Council; Science... amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Heliophysics Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science Committee of the NAC...

75 FR 74089 - NASA Advisory Council; Science Committee; Astrophysics Subcommittee; Meeting

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2010-11-30

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-149)] NASA Advisory Council; Science... amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Astrophysics Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science Committee of the NAC...

75 FR 2892 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2010-01-19

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-001)] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... meeting of the Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee...

75 FR 12310 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

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2010-03-15

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-026)] NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... meeting of the Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee...

78 FR 20358 - NASA Advisory Council; Science Committee; Heliophysics Subcommittee; Meeting

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2013-04-04

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 13-036] NASA Advisory Council; Science... Subcommittee (HPS) of the NASA Advisory Council (NAC). This Subcommittee reports to the Science Committee of... CONTACT: Ms. Marian Norris, Science Mission Directorate, NASA Headquarters, Washington, DC 20546, (202...

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2010-01-19

... Science Committee of the NAC. The Meeting will be held for the purpose of soliciting from the scientific... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-002)] NASA Advisory Council; Science... FURTHER INFORMATION CONTACT: Ms. Marian Norris, Science Mission Directorate, NASA Headquarters, Washington...

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2010-03-22

... Science Committee of the NAC. The Meeting will be held for the purpose of soliciting from the scientific... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-031)] NASA Advisory Council; Science.... FOR FURTHER INFORMATION CONTACT: Ms. Marian Norris, Science Mission Directorate, NASA Headquarters...

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2010-05-28

... Science Committee of the NAC. The Meeting will be held for the purpose of soliciting from the scientific... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-061)] NASA Advisory Council; Science...: Ms. Marian Norris, Science Mission Directorate, NASA Headquarters, Washington, DC 20546, (202) 358...

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2010-01-19

... Science Committee of the NAC. The Meeting will be held for the purpose of soliciting from the scientific... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-004)] NASA Advisory Council; Science..., Washington, DC 20546. FOR FURTHER INFORMATION CONTACT: Ms. Marian Norris, Science Mission Directorate, NASA...

75 FR 19661 - NASA Advisory Council; Science Committee; Planetary Protection Subcommittee; Meeting

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2010-04-15

...). This Subcommittee reports to the Science Committee of the NAC. The meeting will be held for the purpose... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-044)] NASA Advisory Council; Science... 20546. FOR FURTHER INFORMATION CONTACT: Ms. Marian Norris, Science Mission Directorate, NASA...

78 FR 49296 - NASA Advisory Council; Science Committee; Astrophysics Subcommittee; Meeting.

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2013-08-13

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-092] NASA Advisory Council; Science Committee; Astrophysics Subcommittee; Meeting. AGENCY: National Aeronautics and Space Administration. ACTION... of the Astrophysics Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the...

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2012-12-03

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (12-104)] NASA Advisory Council; Science Committee; Planetary Protection Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration... Planetary Protection Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the...

NACS Store Planning Manual.

ERIC Educational Resources Information Center

College Store Journal, 1979

1979-01-01

Topics discussed by the NACS Store Planning/Renovation Committees in this updated version of the college store renovation manual include: short- and long-range planning, financial considerations, professional planning assistance, the store's image and business character, location considerations, building requirements, space requirements, fixtures,…

76 FR 31641 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-01

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 11-050] NASA Advisory Council; Science...-463, as amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

76 FR 58303 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting.

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2011-09-20

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-081)] NASA Advisory Council; Science...-463, as amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

78 FR 77719 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-24

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-156] NASA Advisory Council; Science...-463, as amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Planetary Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the Science...

78 FR 39341 - NASA Advisory Council; Science Committee; Planetary Science Subcommittee; Meeting.

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2013-07-01

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 13-070] NASA Advisory Council; Science..., the National Aeronautics and Space Administration (NASA) announces a meeting of the Planetary Science Subcommittee of the NASA Advisory Council (NAC). This [[Page 39342

75 FR 8997 - NASA Advisory Council; Science Committee; Earth Science Subcommittee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-26

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-021)] NASA Advisory Council; Science...: Notice of meeting. SUMMARY: The National Aeronautics and Space Administration (NASA) announces a meeting of the Earth Science Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to...

A novel wheat NAC transcription factor, TaNAC30, negatively regulates resistance of wheat to stripe rust.

PubMed

Wang, Bing; Wei, Jinping; Song, Na; Wang, Ning; Zhao, Jing; Kang, Zhensheng

2018-05-01

NAC transcription factors are widespread in the plant kingdom and play essential roles in the transcriptional regulation of defense responses. In this study, we isolated a novel NAC transcription factor gene, TaNAC30, from a cDNA library constructed from wheat (Triticum aestivum) plants inoculated with the stripe rust pathogen Puccinia striiformis f. sp. tritici (Pst). TaNAC30 contains a typical NAM domain and localizes to the nucleus. Yeast one-hybrid assays revealed that TaNAC30 exhibits transcriptional activity and that its C-terminus is necessary for the activation of transcription. Expression of TaNAC30 increased when host plants were infected with a virulent race (CYR31) of the rust fungus Pst. Silencing of TaNAC30 by virus-induced gene silencing inhibited colonization of the virulent Pst isolate CYR31. Moreover, detailed histological analyses showed that silencing of TaNAC30 enhanced resistance to Pst by inducing a significant increase in the accumulation of H 2 O 2 . Finally, we overexpressed TaNAC30 in fission yeast and determined that cell viability was severely reduced in TaNAC30-transformed cells grown on medium containing H 2 O 2 . These results suggest that TaNAC30 negatively regulates plant resistance in a compatible wheat-Pst interaction. © 2017 Institute of Botany, Chinese Academy of Sciences.

76 FR 2150 - NASA Advisory Council; Education and Public Outreach Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-12

... Outreach Committee of the NASA Advisory Council (NAC). DATES: Tuesday, February 8, 2011, 9 a.m. to 5 p.m... Recommendations Status Joint Recommendation with the NAC Commercial Space Committee Committee Work Plan Action...

77 FR 5246 - Basic Energy Sciences Advisory Committee

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2012-02-02

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Office of Science... of the Basic Energy Sciences Advisory Committee (BESAC). The Federal Advisory Committee Act (Pub. L... FURTHER INFORMATION CONTACT: Katie Perine; Office of Basic Energy Sciences; U.S. Department of Energy...

78 FR 2259 - Fusion Energy Sciences Advisory Committee

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2013-01-10

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Office of Science... Energy Sciences Advisory Committee. The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat. 770... Energy Sciences; U.S. Department of Energy; 1000 Independence Avenue SW.; Washington, DC 20585-1290...

77 FR 485 - Fusion Energy Sciences Advisory Committee

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2012-01-05

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Office of Science... Energy Sciences Advisory Committee (FESAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat... Sciences; U.S. Department of Energy; 1000 Independence Avenue SW., Washington, DC 20585-1290; Telephone...

76 FR 48147 - Basic Energy Sciences Advisory Committee

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2011-08-08

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of renewal of the Basic Energy Sciences Advisory Committee. SUMMARY... that the Basic Energy Sciences Advisory Committee will be renewed for a two-year period beginning July...

78 FR 6088 - Basic Energy Sciences Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-29

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Office of Science... Energy Sciences Advisory Committee (BESAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat... INFORMATION CONTACT: Katie Perine, Office of Basic Energy Sciences, U.S. Department of Energy; SC-22...

77 FR 38680 - NASA Advisory Council; Human Exploration and Operations Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-28

... persons, scientific and technical information relevant to program planning. DATES: Monday, July 23, 2012... Goddard Space Flight Center (GSFC), Building 1, Room E100D, 8800 Greenbelt Road, Greenbelt, MD 20771. FOR... Session with the NAC Science Committee on Mars Program Planning Group and Joint Robotic Precursor...

76 FR 40714 - Fusion Energy Sciences Advisory Committee

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2011-07-11

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Department of Energy... Energy Sciences Advisory Committee. The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat. 770... Fusion Energy Sciences; U.S. Department of Energy; 1000 Independence Avenue, SW., Washington, DC 20585...

75 FR 6369 - Basic Energy Sciences Advisory Committee

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2010-02-09

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office... Energy Sciences Advisory Committee (BESAC). Federal Advisory Committee Act (Pub. L. 92- 463, 86 Stat. 770...: Katie Perine; Office of Basic Energy Sciences; U.S. Department of Energy; Germantown Building...

78 FR 15937 - Fusion Energy Sciences Advisory Committee

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2013-03-13

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of open meeting. SUMMARY: This notice announces a meeting of the Fusion Energy Sciences Advisory Committee. The Federal Advisory Committee Act requires that public notice of...

78 FR 38696 - Basic Energy Sciences Advisory Committee

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2013-06-27

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office... Energy Sciences Advisory Committee (BESAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat... INFORMATION CONTACT: Katie Perine; Office of Basic Energy Sciences; U.S. Department of Energy; Germantown...

75 FR 8685 - Fusion Energy Sciences Advisory Committee

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2010-02-25

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of open meeting. SUMMARY: This notice announces a meeting of the Fusion Energy Sciences Advisory Committee. The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat. 770...

76 FR 41234 - Basic Energy Sciences Advisory Committee

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2011-07-13

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of open meeting. SUMMARY: This notice announces a meeting of the Basic Energy Sciences Advisory Committee (BESAC). Federal Advisory Committee Act (Pub. L. 92- 463, 86 Stat. 770...

77 FR 41395 - Basic Energy Sciences Advisory Committee

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2012-07-13

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of open meeting. SUMMARY: This notice announces a meeting of the Basic Energy Sciences Advisory Committee (BESAC). Federal Advisory Committee Act (Pub. L. 92- 463, 86 Stat. 770...

75 FR 41838 - Basic Energy Sciences Advisory Committee

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2010-07-19

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of Open Meeting. SUMMARY: This notice announces a meeting of the Basic Energy Sciences Advisory Committee (BESAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat...

76 FR 8358 - Basic Energy Sciences Advisory Committee

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2011-02-14

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Department of Energy, Office of Science. ACTION: Notice of open meeting. SUMMARY: This notice announces a meeting of the Basic Energy Sciences Advisory Committee (BESAC). Federal Advisory Committee Act (Pub. L. 92- 463, 86 Stat. 770...

77 FR 40646 - NASA Advisory Council; Technology and Innovation Committee; Meeting Amendment

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-10

... and Innovation Committee; Meeting Amendment AGENCY: National Aeronautics and Space Administration... meeting of the Technology and Innovation Committee of the NASA Advisory Council (NAC). DATES: Tuesday... that they are attending the NAC's Technology and Innovation Committee meeting in Building 8. All U.S...

78 FR 41804 - NASA Advisory Council; Audit, Finance and Analysis Committee; Meeting.

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-11

...In accordance with the Federal Advisory Committee Act, Public Law 92-463, as amended, the National Aeronautics and Space Administration announces a meeting of the Audit, Finance and Analysis Committee of the NASA Advisory Council (NAC). This Committee reports to the NAC.

76 FR 49757 - Fusion Energy Sciences Advisory Committee

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2011-08-11

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Office of Science... Services Administration, notice is hereby given that the Fusion Energy Sciences Advisory Committee will be... science, fusion science, and fusion technology related to the Fusion Energy Sciences program. Additionally...

78 FR 48863 - Fusion Energy Sciences Advisory Committee

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2013-08-12

... DEPARTMENT OF ENERGY Fusion Energy Sciences Advisory Committee AGENCY: Office of Science... Services Administration, notice is hereby given that the Fusion Energy Sciences Advisory Committee will be... of Science (DOE), on long-range plans, priorities, and strategies for advancing plasma science...

78 FR 12044 - DOE/NSF Nuclear Science Advisory Committee

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2013-02-21

... DEPARTMENT OF ENERGY DOE/NSF Nuclear Science Advisory Committee AGENCY: Office of Science... Nuclear Science Advisory Committee (NSAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86 Stat... Energy and the National Science Foundation on scientific priorities within the field of basic nuclear...

77 FR 67028 - NASA Advisory Council; Commercial Space Committee; Meeting

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2012-11-08

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-093] NASA Advisory Council; Commercial..., the National Aeronautics and Space Administration (NASA) announces a meeting of the Commercial Space Committee of the NASA Advisory Council (NAC). This Committee reports to the NAC. The [[Page 67029

78 FR 10213 - NASA Advisory Council; Commercial Space Committee; Meeting

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2013-02-13

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-012] NASA Advisory Council; Commercial..., the National Aeronautics and Space Administration (NASA) announces a meeting of the Commercial Space Committee of the NASA Advisory Council (NAC). This Committee reports to the NAC. The meeting will be held...

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2013-07-15

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (13-080)] NASA Advisory Council; Commercial..., the National Aeronautics and Space Administration (NASA) announces a meeting of the Commercial Space Committee of the NASA Advisory Council (NAC). This Committee reports to the NAC. The meeting will be held...

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2012-06-28

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (12-052)] NASA Advisory Council; Commercial..., the National Aeronautics and Space Administration (NASA) announces a meeting of the Commercial Space Committee of the NASA Advisory Council (NAC). This Committee reports to the NAC. The meeting will be held...

77 FR 51791 - DOE/NSF Nuclear Science Advisory Committee

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2012-08-27

... DEPARTMENT OF ENERGY DOE/NSF Nuclear Science Advisory Committee AGENCY: Department of Energy.../NSF Nuclear Science Advisory Committee (NSAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86... on scientific priorities within the field of basic nuclear science research. Tentative Agenda: Agenda...

75 FR 6651 - DOE/NSF Nuclear Science Advisory Committee

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2010-02-10

... DEPARTMENT OF ENERGY DOE/NSF Nuclear Science Advisory Committee AGENCY: Department of Energy.../NSF Nuclear Science Advisory Committee (NSAC). Federal Advisory Committee Act (Pub. L. 92- 463, 86... on scientific priorities within the field of basic nuclear science research. Tentative Agenda: Agenda...

76 FR 31945 - DOE/NSF Nuclear Science Advisory Committee

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2011-06-02

... DEPARTMENT OF ENERGY DOE/NSF Nuclear Science Advisory Committee AGENCY: Department of Energy.../NSF Nuclear Science Advisory Committee (NSAC). The Federal Advisory Committee Act (Pub. L. 92-463, 86... the field of basic nuclear science research. Tentative Agenda: Agenda will include discussions of the...

75 FR 18516 - Homeland Security Science and Technology Advisory Committee

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2010-04-12

... Technology Advisory Committee AGENCY: Science and Technology Directorate, DHS. ACTION: Committee Management; notice of closed Federal Advisory Committee meeting SUMMARY: The Homeland Security Science and Technology.... DATES: The Homeland Security Science and Technology Advisory Committee will meet April 20, 2010 from 8...

Citrullus colocynthis NAC transcription factors CcNAC1 and CcNAC2 are involved in light and auxin signaling.

PubMed

Wang, Zhuoyu; Rashotte, Aaron M; Dane, Fenny

2014-10-01

Two novel NAC transcription factors from C itrullus colocynthis implicated in light and auxin signaling pathway. NAC transcription factors (NAM, ATAF1, 2, CUC2) have multiple functions in plant growth and development. Two NACs, CcNAC1 and CcNAC2, were recently identified in the highly drought-tolerant cucurbit species, Citrullus colocynthis. This study examines the functional role of these genes under different qualities of light based on the in silico analysis of the CcNAC1 and CcNAC2 promoters that revealed the presence of several light-associated motifs. The impact of both light and auxin on CcNAC1 and CcNAC2 expression was examined in C. colocynthis leaves, and using reporter (pCcNAC1, 2::GUS) lines in Arabidopsis. Furthermore, the effects of constitutive overexpression (OE-CcNAC1, 2) in Arabidopsis were also examined under a range of conditions to confirm reporter line linkages. White, blue, red, and far-red light treatments resulted in similar patterns of quantitative changes in CcNAC1and CcNAC2 expression in both species, with the highest transcript increases following red light. Photomorphogenic changes in Arabidopsis hypocotyls were correlated with gene transcript levels. In the absence of light, hypocotyls of OE-CcNAC1/CcNAC2 lines were significantly longer as compared to WT. The addition of exogenous auxin (+IAA) to growth medium also resulted in changes to the hypocotyl lengths of overexpression lines and spatiotemporal reporter line changes in seedlings. Our data suggest that CcNAC1, 2 might be functionally important in the light signaling pathway, and appear connected to the hormone auxin. This is the first study to indicate that NAC genes might play a role in both light and auxin signaling pathways.

76 FR 41274 - Committee Name: Homeland Security Science and Technology Advisory Committee (HSSTAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-13

..., cyber-security, knowledge management and how best to leverage related technologies funded by other... Science and Technology Advisory Committee (HSSTAC) ACTION: Committee Management; Notice of Federal... Committee Management Secretariat, General Services Administration. Name of Committee: Homeland Security...

77 FR 19740 - Biological Sciences Advisory Committee; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-02

... NATIONAL SCIENCE FOUNDATION Biological Sciences Advisory Committee; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L., 92- 463, as amended), the National Science Foundation announces the following meeting: Name: Biological Sciences Advisory Committee ( 1110). Date and...

A diverse range of bacterial and eukaryotic chitinases hydrolyzes the LacNAc (Galβ1-4GlcNAc) and LacdiNAc (GalNAcβ1-4GlcNAc) motifs found on vertebrate and insect cells.

PubMed

Frederiksen, Rikki F; Yoshimura, Yayoi; Storgaard, Birgit G; Paspaliari, Dafni K; Petersen, Bent O; Chen, Kowa; Larsen, Tanja; Duus, Jens Ø; Ingmer, Hanne; Bovin, Nicolai V; Westerlind, Ulrika; Blixt, Ola; Palcic, Monica M; Leisner, Jørgen J

2015-02-27

There is emerging evidence that chitinases have additional functions beyond degrading environmental chitin, such as involvement in innate and acquired immune responses, tissue remodeling, fibrosis, and serving as virulence factors of bacterial pathogens. We have recently shown that both the human chitotriosidase and a chitinase from Salmonella enterica serovar Typhimurium hydrolyze LacNAc from Galβ1-4GlcNAcβ-tetramethylrhodamine (LacNAc-TMR (Galβ1-4GlcNAcβ(CH2)8CONH(CH2)2NHCO-TMR)), a fluorescently labeled model substrate for glycans found in mammals. In this study we have examined the binding affinities of the Salmonella chitinase by carbohydrate microarray screening and found that it binds to a range of compounds, including five that contain LacNAc structures. We have further examined the hydrolytic specificity of this enzyme and chitinases from Sodalis glossinidius and Polysphondylium pallidum, which are phylogenetically related to the Salmonella chitinase, as well as unrelated chitinases from Listeria monocytogenes using the fluorescently labeled substrate analogs LacdiNAc-TMR (GalNAcβ1-4GlcNAcβ-TMR), LacNAc-TMR, and LacNAcβ1-6LacNAcβ-TMR. We found that all chitinases examined hydrolyzed LacdiNAc from the TMR aglycone to various degrees, whereas they were less active toward LacNAc-TMR conjugates. LacdiNAc is found in the mammalian glycome and is a common motif in invertebrate glycans. This substrate specificity was evident for chitinases of different phylogenetic origins. Three of the chitinases also hydrolyzed the β1-6 bond in LacNAcβ1-6LacNAcβ-TMR, an activity that is of potential importance in relation to mammalian glycans. The enzymatic affinities for these mammalian-like structures suggest additional functional roles of chitinases beyond chitin hydrolysis. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

78 FR 47677 - Basic Energy Sciences Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-06

... DEPARTMENT OF ENERGY Basic Energy Sciences Advisory Committee AGENCY: Office of Science, Department of Energy. ACTION: Notice of renewal. SUMMARY: Pursuant to Section 14(a)(2)(A) of the Federal... hereby given that the Basic Energy Sciences Advisory Committee's (BESAC) charter will be renewed for a...

78 FR 62609 - DOE/NSF Nuclear Science Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-22

... Secretariat, General Services Administration, notice is hereby given that the DOE/NSF Nuclear Science Advisory Committee (NSAC) will be renewed for a two-year period. The Committee will provide advice and... research. Additionally, the renewal of the DOE/NSF Nuclear Science Advisory Committee has been determined...

The National Astronomy Consortium (NAC) - Overview

NASA Astrophysics Data System (ADS)

Sheth, Kartik; Mills, Elisabeth A. C.; Hooper, Eric; National Astronomy Consortium

2015-01-01

The National Astronomy Consortium (NAC; see https://sites.google.com/site/nraonac/) is a growing national partnership between majority and minority universities and institutions with the goal of increasing the numbers of under-represented minorities and students who might otherwise be overlooked by the traditional academic pipeline into STEM, or related, careers. The NAC model is based on the successful 'Posse Foundation' model for undergraduate success and incorporates all its major components: pre-training of cohorts to prepare them for the research experience, joint weekly cohort activities throughout the research summer, peer- and multiple mentoring, weekly discussion of various aspects of professional and career development, continued engagement of students in science after return to home institution and lifelong mentoring. The mentors also form a cohort, exchanging information and learning from each other. With its partner institutions, the NAC aims to build a complete pipeline from undergraduate through career for the next generation of scientists and engineers. Our annual goal is to create two to three cohorts of four to five students at each site (currently NRAO-Charlottesville, NRAO-Socorro and U. Wisconsin - Madison). Recruitment occurs in the fall semester with seminars and colloquia in partnership with faculty at the minority serving institutions and the GRAD-MAP program at the University of Maryland. In this talk we describe in detail all the components of the NAC and report on our progress. We are keen to interact and partner with new universities and institutions and encourage them to contact the NAC at nac4stem@googlegroups.com.

75 FR 39955 - Homeland Security Science and Technology Advisory Committee

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2010-07-13

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78 FR 31977 - NASA Applied Sciences Advisory Committee Meeting

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Committee on Women in Science, Engineering, and Medicine (CWSEM)

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Skip to Main Content Contact Us | Search: Search The National Academies of Sciences, Engineering and Medicine Committee on Women in Science, Engineering, and Medicine Committee on Women in Science , Engineering, and Medicine Policy and Global Affairs Home About Us Members Subscribe to CWSEM Alerts Resources

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2010-03-09

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New GlcNAc/GalNAc-specific lectin from the ascidian Didemnum ternatanum.

PubMed

Molchanova, Valentina; Chikalovets, Irina; Li, Wei; Kobelev, Stanislav; Kozyrevskaya, Svetlana; Bogdanovich, Raisa; Howard, Eric; Belogortseva, Natalia

2005-05-25

Previously we isolated GlcNAc-specific lectin (DTL) from the ascidian Didemnum ternatanum by affinity chromatography on cross-linked ovalbumin. Here we report the purification and characterization of new D-GlcNAc/D-GalNAc-specific lectin DTL-A from the same ascidian. This lectin was isolated from non-bound cross-linked ovalbumin fraction and further was purified by gel filtration on Sepharose CL-4B, affinity chromatography on GlcNAc-agarose and gel filtration on Superdex 200. SDS-polyacrylamide gel electrophoresis and gel filtration of purified lectin on Sepharose CL-4B indicates that it exists as large aggregates in the native state. Investigations of the carbohydrate specificity of DTL-A by enzyme-linked lectin assay suggest the multi-specificity of this lectin. DTL-A binds BSM, asialo-BSM as well as heparin and dextran sulfate. The binding of DTL-A to BSM was inhibited by monosaccharides D-GlcNAc and D-GalNAc, their alpha- but not beta-anomers. Among polysaccharides and glycoconjugates, DTL-A binding to BSM was effectively inhibited by BSM, asialo-BSM, pronase-treated BSM and synthetic alpha-D-GalNAc-PAA. Fetuin and asialofetuin showed a much lower inhibitory potency, heparin and dextran sulfate were noninhibitory. On the other hand, DTL-A binding to heparin was effectively inhibited by dextran sulfate, fucoidan, whereas BSM showed insignificantly inhibitory effect. DTL-A binding to heparin was not inhibited by D-GlcNAc and D-GalNAc.

75 FR 57079 - NASA Advisory Council; Information Technology Infrastructure Committee; Meeting

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2010-09-17

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76 FR 38430 - Subcommittee on Forensic Science; Committee on Science; National Science and Technology Council

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2011-06-30

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Subcommittee on Forensic Science; Committee on Science; National Science and Technology Council ACTION: Notice of Panel Session. Public input is requested... Sciences 2009 report: ``Strengthening Forensic Science in the United States: A Path Forward'' ( http://www...

Synthesis of aryl azide derivatives of UDP-GlcNAc and UDP-GalNAc and their use for the affinity labeling of glycosyltransferases and the UDP-HexNAc pyrophosphorylase.

PubMed

Zeng, Y; Shabalin, Y; Szumilo, T; Pastuszak, I; Drake, R R; Elbein, A D

1996-07-15

The chemical synthesis and utilization of two photoaffinity analogs, 125I-labeled 5-[3-(p-azidosalicylamido)-1-propenyl]-UDP-GlcNAc and -UDP-GalNAc, is described. Starting with either UDP-GlcNAc or UDP-GalNAc, the synthesis involved the preparation of the 5-mercuri-UDP-HexNAc and then attachment of an allylamine to the 5 position to give 5-(3-amino)allyl-UDP-HexNAc. This was followed by acylation with N-hydroxysuccinimide p-aminosalicylic acid to form the final product, i.e., 5-[3-(p-azidosalicylamido)-1-propenyl]-UDP-GlcNAc or UDP-GalNAc. These products could then be iodinated with chloramine T to give the 125I-derivatives. Both the UDP-GlcNAc and the UDP-GalNAc derivatives reacted in a concentration-dependent manner with a highly purified UDP-HexNAc pyrophosphorylase, and both specifically labeled the subunit(s) of this protein. The labeling of the protein by the UDP-GlcNAc derivative was inhibited in dose-dependent fashion by either unlabeled UDP-GlcNAc or unlabeled UDP-GalNAc. Likewise, labeling with the UDP-GalNAc probe was blocked by either UDP-GlcNAc or UDP-GalNAc. The UDP-GlcNAc probe also specifically labeled a partially purified preparation of GlcNAc transferase I.

78 FR 12033 - Census Advisory Committees

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2013-02-21

... Other Populations (NAC). The Committee will address census policies, research and methodology, tests..., methodological, geographic, behavioral, and operational variables affecting the cost, accuracy, and...

76 FR 6163 - Subcommittee on Forensic Science; Committee on Science; National Science and Technology Council

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2011-02-03

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Subcommittee on Forensic Science; Committee on Science; National Science and Technology Council ACTION: Notice of meeting. Public input is requested concerning... Forensic Science can be obtained through the Office of Science and Technology Policy's NSTC Web site at...

78 FR 14101 - Homeland Security Science and Technology Advisory Committee (HSSTAC)

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2013-03-04

... Technology Advisory Committee (HSSTAC) AGENCY: Science and Technology Directorate, DHS. ACTION: Committee... Technology Advisory Committee (HSSTAC) will meet on March 21, 2013 in Washington, DC The meeting will be open... the Department of Homeland Security (DHS), Science and Technology Directorate, 1120 Vermont Avenue NW...

78 FR 42553 - NASA Advisory Council; Information Technology Infrastructure Committee; Meeting

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2013-07-16

...; Information Technology Infrastructure Committee; Meeting AGENCY: National Aeronautics and Space Administration... Information Technology Infrastructure Committee (ITIC) of the NASA Advisory Council (NAC). This Committee..., DC 20546. FOR FURTHER INFORMATION CONTACT: Ms. Deborah Diaz, ITIC Executive Secretariat, NASA...

75 FR 4882 - Subcommittee on Forensic Science; Committee on Science; National Science and Technology Council

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2010-01-29

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78 FR 21421 - NASA Advisory Council; Science Committee; Planetary Protection Subcommittee; Meeting

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2013-04-10

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 13-048] NASA Advisory Council; Science...-463, as amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Planetary Protection Subcommittee of the NASA Advisory Council (NAC). This Subcommittee reports to the...

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2011-03-25

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76 FR 38188 - Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting

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2011-06-29

...] Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting AGENCY: Food and... of Committee: Advisory Committee for Pharmaceutical Science and Clinical Pharmacology. General..., 2011, the committee will discuss current strategies for FDA's Office of Pharmaceutical Science...

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2011-07-11

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2011-07-01

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2013-09-23

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2011-04-14

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-040)] NASA Advisory Council; Science Committee; Earth Science Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION... amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Earth Science...

75 FR 65673 - NASA Advisory Council; Science Committee; Earth Science Subcommittee; Meeting

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2010-10-26

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2012-05-09

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2012-09-20

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2013-08-22

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2013-03-26

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2011-08-10

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2010-07-19

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2012-02-28

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Michael Griffin House Science Committee Hearing

NASA Image and Video Library

2005-06-27

NASA Administrator Michael Griffin prepares to testify at a hearing before the House Science Committee, Tuesday, June 28, 2005, Rayburn House Office building, Washington. Photo Credit: (NASA/Bill Ingalls)

House Science Committee Acts on NIST Laboratories

NASA Astrophysics Data System (ADS)

Committees in both chambers of Congress are considering bills to dismantle the Department of Commerce, including the National Oceanic and Atmospheric Administration. The main bill in the House, HR 1756, sponsored by Rep. Dick Chrysler (R-Mich.), has been referred to 11 different committees, each of which has jurisdiction over some part of it. In September, the House Science Committee, chaired by Robert Walker (R-Pa.), held a hearing on the proposal and marked up the portions of Chrysler's bill relating to science and technology.In addition to abolishing the Commerce Department, Chrysler's bill would mandate selling NIST's laboratories and those of NOAA's Office of Oceanic and Atmospheric Research "to a private sector entity intending to perform substantially the same functions as were performed by the laboratories." It also would terminate NIST's Advanced Technology Program (ATP) and Manufacturing Extension Partnership (MEP).

Committee on Atomic, Molecular and Optical Sciences

DOE Office of Scientific and Technical Information (OSTI.GOV)

Lancaster, James

The Committee on Atomic, Molecular, and Optical Sciences (CAMOS) is a standing activity of the National Research Council (NRC) that operates under the auspices of the Board on Physics and Astronomy. CAMOS is one of five standing committees of the BPA that are charged with assisting it in achieving its goals—monitoring the health of physics and astronomy, identifying important new developments at the scientific forefronts, fostering interactions with other fields, strengthening connections to technology, facilitating effective service to the nation, and enhancing education in physics. CAMOS provides these capabilities for the atomic, molecular and optical (AMO) sciences.

Michael Griffin House Science Committee Hearing

NASA Image and Video Library

2005-06-27

Rep. Bart Gordon, D-TN., questions NASA Administrator Michael Griffin during a House Science Committee hearing, Tuesday, June 28, 2005, Rayburn House Office building, Washington. Photo Credit: (NASA/Bill Ingalls)

78 FR 57128 - Census Advisory Committees; Meetings

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2013-09-17

... Other Populations (NAC). The Committee will address census policies, research and methodology, tests..., methodological, geographic, behavioral and operational variables affecting the cost, accuracy and implementation...

Cloning and characterization of a novel NAC family gene CarNAC1 from chickpea (Cicer arietinum L.).

PubMed

Peng, Hui; Yu, Xingwang; Cheng, Huiying; Shi, Qinghua; Zhang, Hua; Li, Jiangui; Ma, Hao

2010-01-01

The plant-specific NAC (for NAM, ATAF1,2 and CUC2) proteins have been found to play important roles in plant development and stress responses. In this study, a NAC gene CarNAC1 (for Cicer arietinum L. NAC gene 1) was isolated from a cDNA library constructed with chickpea seedling leaves treated by polyethylene glycol. CarNAC1 encoded a putative protein with 239 amino acids and contained 3 exons and 2 introns within genomic DNA sequence. CarNAC1 had a conserved NAC domain in the N-terminus and the CarNAC1:GFP (green fluorescent protein) fusion protein was localized in the nucleus of onion epidermal cells. Additionally, CarNAC1 exhibited the trans-activation activity which was mapped to the C-terminus. The CarNAC1 transcript was detected in many chickpea organs including seedling leaves, stems, roots, flowers, and young pods, but less accumulated in young seeds. CarNAC1 was induced by leaf age and showed changes in expression during seed development and germination. Furthermore, the expression of CarNAC1 was strongly induced by drought, salt, cold, wounding, H(2)O(2), ethephon, salicylic acid, indole-3-acetic acid, and gibberellin. Our results suggest that CarNAC1 encodes a novel NAC-domain protein and may be a transcriptional activator involved in plant development and various stress responses.

75 FR 28275 - Homeland Security Science and Technology Advisory Committee

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2010-05-20

... DEPARTMENT OF HOMELAND SECURITY [Docket No. DHS-2010-0020] Homeland Security Science and...; Notice of Closed Federal Advisory Committee Meeting. SUMMARY: On April 12, 2010, the Homeland Security...: The Homeland Security Science and Technology Advisory Committee met on April 20, 2010 from 8:30 a.m...

Bisecting GlcNAc restricts conformations of branches in model N-glycans with GlcNAc termini.

PubMed

Hanashima, Shinya; Suga, Akitsugu; Yamaguchi, Yoshiki

2018-02-01

Bisected N-glycans play significant roles in tumor migration and Alzheimer's disease through modulating the action and localization of their carrier proteins. Such biological functions are often discussed in terms of the conformation of the attached N-glycans with or without bisecting GlcNAc. To obtain insights into the effects of bisecting GlcNAc on glycan conformation, a systematic NMR structural analysis was performed on two pairs of synthetic N-glycans, with and without bisecting GlcNAc. The analysis reveals that terminal GlcNAcs and bisecting GlcNAc cooperate to restrict the conformations of both the α1-3 and α1-6 branches of N-glycans. 1 H and 13 C chemical shift comparisons suggest that bisecting GlcNAc directly modulates local conformation. Unique NOE correlations between core-mannose and the α1-3 branch mannose as well as the 3 J C-H constant of the glycoside linkage indicate that bisecting GlcNAc restricts the conformation of the 1-3 branch. The angles of the glycosidic bonds between core-mannose and α1-6 branch mannose derived from 3 J C-H and 3 J H-H coupling constants show that terminal GlcNAcs restrict the distribution of the ψ angle to 180° and the bisecting GlcNAc increases the distribution of the ω angle +60° in the presence of terminal GlcNAcs. It is feasible that restriction of branch conformations by bisecting GlcNAc has important consequences for protein-glycan interplay and following biological events. Copyright © 2017 Elsevier Ltd. All rights reserved.

Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis culminating in enucleation.

PubMed

Furuta, Kaori Miyashima; Yadav, Shri Ram; Lehesranta, Satu; Belevich, Ilya; Miyashima, Shunsuke; Heo, Jung-ok; Vatén, Anne; Lindgren, Ove; De Rybel, Bert; Van Isterdael, Gert; Somervuo, Panu; Lichtenberger, Raffael; Rocha, Raquel; Thitamadee, Siripong; Tähtiharju, Sari; Auvinen, Petri; Beeckman, Tom; Jokitalo, Eija; Helariutta, Ykä

2014-08-22

Photoassimilates such as sugars are transported through phloem sieve element cells in plants. Adapted for effective transport, sieve elements develop as enucleated living cells. We used electron microscope imaging and three-dimensional reconstruction to follow sieve element morphogenesis in Arabidopsis. We show that sieve element differentiation involves enucleation, in which the nuclear contents are released and degraded in the cytoplasm at the same time as other organelles are rearranged and the cytosol is degraded. These cellular reorganizations are orchestrated by the genetically redundant NAC domain-containing transcription factors, NAC45 and NAC86 (NAC45/86). Among the NAC45/86 targets, we identified a family of genes required for enucleation that encode proteins with nuclease domains. Thus, sieve elements differentiate through a specialized autolysis mechanism. Copyright © 2014, American Association for the Advancement of Science.

Michael Griffin House Science Committee Hearing

NASA Image and Video Library

2005-06-27

Members of the media photograph NASA Administrator Michael Griffin as he testifies at a hearing before the House Science Committee, Tuesday, June 28, 2005, Rayburn House Office building, Washington. Photo Credit: (NASA/Bill Ingalls)

75 FR 11551 - Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting

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2010-03-11

...] Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting AGENCY: Food and... of Committee: Advisory Committee for Pharmaceutical Science and Clinical Pharmacology. General... Pharmaceutical Science (OPS) on the regulatory challenges of drug-induced phospholipidosis (excessive...

MICHIGAN SCIENCE CURRICULUM COMMITTEE JUNIOR HIGH SCHOOL PROJECT. (TITLE SUPPLIED).

ERIC Educational Resources Information Center

VAN DEVENTER, W.C.

REPORTED ARE THE RESULTS OF A CURRICULUM RESEARCH PROJECT OF THE MICHIGAN SCIENCE CURRICULUM COMMITTEE JUNIOR HIGH SCHOOL PROJECT FOR USE IN TEACHING JUNIOR HIGH SCHOOL UNIFIED SCIENCE. THE COMMITTEE USED PREVIOUS RESEARCH DATA, PARTICULARLY IN THE AREA OF INSTRUCTION AND INQUIRY TRAINING, TO DEVELOP 13 UNITS INCLUDING 55 OPEN-ENDED LABORATORY…

78 FR 17219 - Homeland Security Science and Technology Advisory Committee Meeting Cancellation

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2013-03-20

... DEPARTMENT OF HOMELAND SECURITY [Docket No. DHS-2013-0014] Homeland Security Science and...: Notice of Cancellation of the Federal Advisory Committee Meeting for Homeland Security Science and Technology Advisory Committee (HSSTAC). SUMMARY: The meeting of the Homeland Security Science and Technology...

Novel NAC Transcription Factor TaNAC67 Confers Enhanced Multi-Abiotic Stress Tolerances in Arabidopsis

PubMed Central

Mao, Xinguo; Chen, Shuangshuang; Li, Ang; Zhai, Chaochao; Jing, Ruilian

2014-01-01

Abiotic stresses are major environmental factors that affect agricultural productivity worldwide. NAC transcription factors play pivotal roles in abiotic stress signaling in plants. As a staple crop, wheat production is severely constrained by abiotic stresses whereas only a few NAC transcription factors have been characterized functionally. To promote the application of NAC genes in wheat improvement by biotechnology, a novel NAC gene designated TaNAC67 was characterized in common wheat. To determine its role, transgenic Arabidopsis overexpressing TaNAC67-GFP controlled by the CaMV-35S promoter was generated and subjected to various abiotic stresses for morphological and physiological assays. Gene expression showed that TaNAC67 was involved in response to drought, salt, cold and ABA treatments. Localization assays revealed that TaNAC67 localized in the nucleus. Morphological analysis indicated the transgenics had enhanced tolerances to drought, salt and freezing stresses, simultaneously supported by enhanced expression of multiple abiotic stress responsive genes and improved physiological traits, including strengthened cell membrane stability, retention of higher chlorophyll contents and Na+ efflux rates, improved photosynthetic potential, and enhanced water retention capability. Overexpression of TaNAC67 resulted in pronounced enhanced tolerances to drought, salt and freezing stresses, therefore it has potential for utilization in transgenic breeding to improve abiotic stress tolerance in crops. PMID:24427285

75 FR 81678 - Aeronautics Science and Technology Subcommittee; Committee on Technology; National Science and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-12-28

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Aeronautics Science and Technology Subcommittee; Committee on Technology; National Science and Technology Council ACTION: Notice of Meeting--Public input is.... SUMMARY: The Aeronautics Science and Technology Subcommittee (ASTS) of the National Science and Technology...

The National Astronomy Consortium (NAC)

NASA Astrophysics Data System (ADS)

Von Schill, Lyndele; Ivory, Joyce

2017-01-01

The National Astronomy Consortium (NAC) program is designed to increase the number of underrepresented minority students into STEM and STEM careers by providing unique summer research experiences followed by long-term mentoring and cohort support. Hallmarks of the NAC program include: research or internship opportunities at one of the NAC partner sites, a framework to continue research over the academic year, peer and faculty mentoring, monthly virtual hangouts, and much more. NAC students also participate in two professional travel opportunities each year: the annual NAC conference at Howard University and poster presentation at the annual AAS winter meeting following their summer internship.The National Astronomy Consortium (NAC) is a program led by the National Radio Astronomy Consortium (NRAO) and Associated Universities, Inc. (AUI), in partnership with the National Society of Black Physicist (NSBP), along with a number of minority and majority universities.

TaNAC29, a NAC transcription factor from wheat, enhances salt and drought tolerance in transgenic Arabidopsis.

PubMed

Huang, Quanjun; Wang, Yan; Li, Bin; Chang, Junli; Chen, Mingjie; Li, Kexiu; Yang, Guangxiao; He, Guangyuan

2015-11-04

NAC (NAM, ATAF, and CUC) transcription factors play important roles in plant biological processes, including phytohormone homeostasis, plant development, and in responses to various environmental stresses. TaNAC29 was introduced into Arabidopsis using the Agrobacterium tumefaciens-mediated floral dipping method. TaNAC29-overexpression plants were subjected to salt and drought stresses for examining gene functions. To investigate tolerant mechanisms involved in the salt and drought responses, expression of related marker genes analyses were conducted, and related physiological indices were also measured. Expressions of genes were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). A novel NAC transcription factor gene, designated TaNAC29, was isolated from bread wheat (Triticum aestivum). Sequence alignment suggested that TaNAC29 might be located on chromosome 2BS. TaNAC29 was localized to the nucleus in wheat protoplasts, and proved to have transcriptional activation activities in yeast. TaNAC29 was expressed at a higher level in the leaves, and expression levels were much higher in senescent leaves, indicating that TaNAC29 might be involved in the senescence process. TaNAC29 transcripts were increased following treatments with salt, PEG6000, H2O2, and abscisic acid (ABA). To examine TaNAC29 function, transgenic Arabidopsis plants overexpressing TaNAC29 were generated. Germination and root length assays of transgenic plants demonstrated that TaNAC29 overexpression plants had enhanced tolerances to high salinity and dehydration, and exhibited an ABA-hypersensitive response. When grown in the greenhouse, TaNAC29-overexpression plants showed the same tolerance response to salt and drought stresses at both the vegetative and reproductive period, and had delayed bolting and flowering in the reproductive period. Moreover, TaNAC29 overexpression plants accumulated lesser malondialdehyde (MDA), H2O2, while had higher superoxide dismutase (SOD) and

Solid State Sciences Committee Forum

DTIC Science & Technology

1992-05-01

3. REPOT TYPE AND CATES COVERED I Final Report 01 Mar 91-29 Feb 92 4. MrlLE AND SUBTITLE S. FUNOG4 NUMBERS SOLID STATE SCIENCES COMMITTEE FORUM AFOSR...lON IU2EM , Appeved kv pub~e We=% I3. ABSTRACT (MaOimum 200 wovij The 1991 SSSC Forum was conductted under the auspices of the Board on Physics and...Astronomy’s Solid State Sciences Committe (SSSC) and cosponsored with the National Materials Advisory Board (NMAB). The Forum was the culmination of a

Ectopic Expression of Pumpkin NAC Transcription Factor CmNAC1 Improves Multiple Abiotic Stress Tolerance in Arabidopsis

PubMed Central

Cao, Haishun; Wang, Li; Nawaz, Muhammad A.; Niu, Mengliang; Sun, Jingyu; Xie, Junjun; Kong, Qiusheng; Huang, Yuan; Cheng, Fei; Bie, Zhilong

2017-01-01

Drought, cold and salinity are the major environmental stresses that limit agricultural productivity. NAC transcription factors regulate the stress response in plants. Pumpkin (Cucurbita moschata) is an important cucurbit vegetable crop and it has strong resistance to abiotic stress; however, the biological functions of stress-related NAC genes in this crop are largely unknown. This study reports the function of CmNAC1, a stress-responsive pumpkin NAC domain protein. The CmNAC1-GFP fusion protein was transiently expressed in tobacco leaves for subcellular localization analysis, and we found that CmNAC1 is localized in the nucleus. Transactivation assay in yeast cells revealed that CmNAC1 functions as a transcription activator, and its transactivation domain is located in the C-terminus. CmNAC1 was ubiquitously expressed in different organs, and its transcript was induced by salinity, cold, dehydration, H2O2, and abscisic acid (ABA) treatment. Furthermore, the ectopic expression (EE) of CmNAC1 in Arabidopsis led to ABA hypersensitivity and enhanced tolerance to salinity, drought and cold stress. In addition, five ABA-responsive elements were enriched in CmNAC1 promoter. The CmNAC1-EE plants exhibited different root architecture, leaf morphology, and significantly high concentration of ABA compared with WT Arabidopsis under normal conditions. Our results indicated that CmNAC1 is a critical factor in ABA signaling pathways and it can be utilized in transgenic breeding to improve the abiotic stress tolerance of crops. PMID:29234347

O-GlcNAc site-mapping of liver X receptor-α and O-GlcNAc transferase.

PubMed

Fan, Qiong; Moen, Anders; Anonsen, Jan Haug; Bindesbøll, Christian; Sæther, Thomas; Carlson, Cathrine Rein; Grønning-Wang, Line M

2018-05-05

The Liver X Receptor α (LXRα) belongs to the nuclear receptor superfamily and plays an essential role in regulating cholesterol, lipid and glucose metabolism and inflammatory responses. We have previously shown that LXRα is post-translationally modified by O-linked β-N-acetyl-glucosamine (O-GlcNAc) with increased transcriptional activity. Moreover, we showed that LXRα associates with O-GlcNAc transferase (OGT) in vitro and in vivo in mouse liver. In this study, we report that human LXRα is O-GlcNAc modified in its N-terminal domain (NTD) by identifying a specific O-GlcNAc site S49 and a novel O-GlcNAc modified peptide 20 LWKPGAQDASSQAQGGSSCILRE 42 . However, O-GlcNAc site-mutations did not modulate LXRα transactivation of selected target gene promoters in vitro. Peptide array and co-immunoprecipitation assays demonstrate that LXRα interacts with OGT in its NTD and ligand-binding domain (LBD) in a ligand-independent fashion. Moreover, we map two new O-GlcNAc sites in the longest OGT isoform (ncOGT): S437 in the tetratricopeptide repeat (TPR) 13 domain and T1043 in the far C-terminus, and a new O-GlcNAc modified peptide (amino acids 826-832) in the intervening region (Int-D) within the catalytic domain. We also map four new O-GlcNAc sites in the short isoform sOGT: S391, T393, S399 and S437 in the TPRs 11-13 domain. Future studies will reveal the biological role of identified O-GlcNAc sites in LXRα and OGT. Copyright © 2018 Elsevier Inc. All rights reserved.

78 FR 72718 - NASA Advisory Council; Information Technology Infrastructure Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-03

...; Information Technology Infrastructure Committee; Meeting AGENCY: National Aeronautics and Space Administration... Information Technology Infrastructure Committee (ITIC) of the NASA Advisory Council (NAC). DATES: Tuesday... Chief Information Officer Space Launch System Kennedy Space Center Operations and Technology Issues...

78 FR 79478 - Advisory Committee on Climate Change and Natural Resource Science

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-30

... announce that the Advisory Committee on Climate Change and Natural Resource Science will hold a meeting..., National Climate Change and Wildlife Science Center, U.S. Geological Survey, 12201 Sunrise Valley Drive...: Chartered in May 2013, the Advisory Committee on Climate Change and Natural Resource Science (ACCCNRS...

75 FR 36722 - Aeronautics Science and Technology Subcommittee; Committee on Technology; National Science and...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-28

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Aeronautics Science and Technology Subcommittee; Committee on Technology; National Science and Technology Council ACTION: Notice of Meeting--Public input is... Evaluation (RDT&E) Infrastructure Plan. SUMMARY: The Aeronautics Science and Technology Subcommittee (ASTS...

75 FR 37783 - DOE/NSF Nuclear Science Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-30

... Science Foundation's Nuclear Physics Office. Technical Talk on Deep Underground Science and Engineering... Energy's Office of Nuclear Physics Web site for viewing. Rachel Samuel, Deputy Committee Management...

Biosynthesis of UDP-GlcNAc, UndPP-GlcNAc and UDP-GlcNAcA Involves Three Easily Distinguished 4-Epimerase Enzymes, Gne, Gnu and GnaB

PubMed Central

Cunneen, Monica M.; Liu, Bin; Wang, Lei; Reeves, Peter R.

2013-01-01

We have undertaken an extensive survey of a group of epimerases originally named Gne, that were thought to be responsible for inter-conversion of UDP-N-acetylglucosamine (UDP-GlcNAc) and UDP-N-acetylgalactosamine (UDP-GalNAc). The analysis builds on recent work clarifying the specificity of some of these epimerases. We find three well defined clades responsible for inter-conversion of the gluco- and galacto-configuration at C4 of different N-acetylhexosamines. Their major biological roles are the formation of UDP-GalNAc, UDP-N-acetylgalactosaminuronic acid (UDP-GalNAcA) and undecaprenyl pyrophosphate-N-acetylgalactosamine (UndPP-GalNAc) from the corresponding glucose forms. We propose that the clade of UDP-GlcNAcA epimerase genes be named gnaB and the clade of UndPP-GlcNAc epimerase genes be named gnu, while the UDP-GlcNAc epimerase genes retain the name gne. The Gne epimerases, as now defined after exclusion of those to be named GnaB or Gnu, are in the same clade as the GalE 4-epimerases for inter-conversion of UDP-glucose (UDP-Glc) and UDP-galactose (UDP-Gal). This work brings clarity to an area that had become quite confusing. The identification of distinct enzymes for epimerisation of UDP-GlcNAc, UDP-GlcNAcA and UndPP-GlcNAc will greatly facilitate allocation of gene function in polysaccharide gene clusters, including those found in bacterial genome sequences. A table of the accession numbers for the 295 proteins used in the analysis is provided to enable the major tree to be regenerated with the inclusion of additional proteins of interest. This and other suggestions for annotation of 4-epimerase genes will facilitate annotation. PMID:23799153

78 FR 716 - DOE/NSF Nuclear Science Advisory Committee

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2013-01-04

... DEPARTMENT OF ENERGY DOE/NSF Nuclear Science Advisory Committee AGENCY: Office of Science, DOE. ACTION: Notice of open meeting. SUMMARY: This notice announces a meeting of the DOE/NSF Nuclear Science... Energy and the National Science Foundation on scientific priorities within the field of basic nuclear...

75 FR 16515 - NASA Advisory Council; Technology and Innovation Committee; Meeting.

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2010-04-01

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-037)] NASA Advisory Council; Technology and Innovation Committee; Meeting. AGENCY: National Aeronautics and Space Administration. ACTION... of the Technology and Innovation Committee of the NASA Advisory Council (NAC). The Meeting will be...

78 FR 41115 - NASA Advisory Council; Technology and Innovation Committee; Meeting.

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2013-07-09

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-073] NASA Advisory Council; Technology and Innovation Committee; Meeting. AGENCY: National Aeronautics and Space Administration. ACTION... of the Technology and Innovation Committee of the NASA Advisory Council (NAC). The meeting will be...

76 FR 20051 - Advisory Committee for International Science & Engineering; Notice of Meeting

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2011-04-11

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science & Engineering; Notice of... Engineering ( 25104). Date/Time: April 25, 2011; 8:30 a.m. to 5 p.m. April 26, 2011; 8:30 a.m. to 12 p.m... International Science and Engineering, Reports from Advisory Committee Working Groups. April 26, 2011 AM...

77 FR 68152 - NASA Advisory Council; Science Committee; Meeting

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2012-11-15

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (12-086)] NASA Advisory Council; Science..., the National Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee.... Marian Norris, Science Mission Directorate, NASA Headquarters, Washington, DC 20546, (202) 358-4452, fax...

77 FR 9705 - NASA Advisory Council; Technology and Innovation Committee; Meeting

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2012-02-17

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-013] NASA Advisory Council; Technology and Innovation Committee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION... and Innovation Committee of the NASA Advisory Council (NAC). DATES: Tuesday, March 6, 2012, 8 a.m. to...

77 FR 38679 - NASA Advisory Council; Audit, Finance and Analysis Committee; Meeting

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2012-06-28

...In accordance with the Federal Advisory Committee Act, Public Law 92-463, as amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Audit, Finance and Analysis Committee of the NASA Advisory Council (NAC).

77 FR 17102 - Advisory Committee for Mathematical and Physical Sciences

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2012-03-23

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences Correction... meeting information is as follows: Name: Directorate for Mathematical and Physical Sciences Advisory... Person: Dr. Morris L. Aizenman, Senior Science Associate, Directorate for Mathematical and Physical...

78 FR 64255 - Advisory Committee for Computer and Information Science and Engineering; Cancellation of Meeting

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2013-10-28

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Computer and Information Science and Engineering; Cancellation of Meeting SUMMARY: As a result of the impact of the recent government shutdown, the... Committee for Computer and Information Science and Engineering meeting. The public notice for this committee...

78 FR 58315 - Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting

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2013-09-23

...] Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting AGENCY: Food and... of Committee: Advisory Committee for Pharmaceutical Science and Clinical Pharmacology. General... continuous manufacturing for pharmaceutical products. Speakers from the Agency, academia, and industry will...

77 FR 13159 - Nanoscale Science, Engineering, and Technology Subcommittee of the Committee on Technology...

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2012-03-05

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering, and Technology Subcommittee of the Committee on Technology, National Science and Technology Council Workshop ACTION: Notice of... Nanoscale Science, Engineering, and Technology (NSET) Subcommittee of the Committee on Technology, National...

76 FR 34103 - President's Committee on the National Medal of Science; Notice of Meeting

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2011-06-10

... NATIONAL SCIENCE FOUNDATION President's Committee on the National Medal of Science; Notice of... Science Foundation announces the following meeting: Name: President's Committee on the National Medal of Science (1182). Date and Time: Wednesday, July 6, 2011, 8:30 a.m.-3:30 p.m. Place: National Science...

78 FR 48724 - President's Committee on the National Medal of Science; Notice of Meeting

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2013-08-09

... NATIONAL SCIENCE FOUNDATION President's Committee on the National Medal of Science; Notice of... Science Foundation announces the following meeting: Name: President's Committee on the National Medal of Science (1182). Date and Time: Tuesday, September 3, 2013, 8:30 a.m.-2 p.m. Place: National Science...

77 FR 61644 - President's Committee on the National Medal of Science Notice of Meeting

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2012-10-10

... NATIONAL SCIENCE FOUNDATION President's Committee on the National Medal of Science Notice of... Science Foundation announces the following meeting: Name: President's Committee on the National Medal of Science (1182). Date and Time: Wednesday, October 31, 2011, 8:30 a.m.-2:00 p.m. Place: National Science...

The Glycine soja NAC transcription factor GsNAC019 mediates the regulation of plant alkaline tolerance and ABA sensitivity.

PubMed

Cao, Lei; Yu, Yang; Ding, Xiaodong; Zhu, Dan; Yang, Fan; Liu, Beidong; Sun, Xiaoli; Duan, Xiangbo; Yin, Kuide; Zhu, Yanming

2017-10-01

Overexpression of Gshdz4 or GsNAC019 enhanced alkaline tolerance in transgenic Arabidopsis. We proved that Gshdz4 up-regulated both GsNAC019 and GsRD29B but GsNAC019 may repress the GsRD29B expression under alkaline stress. Wild soybean (Glycine soja) has a high tolerance to environmental challenges. It is a model species for dissecting the molecular mechanisms of salt-alkaline stresses. Although many NAC transcription factors play important roles in response to multiple abiotic stresses, such as salt, osmotic and cold, their mode of action in alkaline stress resistance is largely unknown. In our study, we identified a G. soja NAC gene, GsNAC019, which is a homolog of the Arabidopsis AtNAC019 gene. GsNAC019 was highly up-regulated by 50 mM NaHCO 3 treatment in the roots of wild soybean. Further investigation showed that a well-characterized transcription factor, Gshdz4 protein, bound the cis-acting element sequences (CAATA/TA), which are located in the promoter of the AtNAC019/GsNAC019 genes. Overexpression of Gshdz4 positively regulated AtNAC019 expression in transgenic Arabidopsis, implying that AtNAC019/GsNAC019 may be the target genes of Gshdz4. GsNAC019 was demonstrated to be a nuclear-localized protein in onion epidermal cells and possessed transactivation activity in yeast cells. Moreover, overexpression of GsNAC019 in Arabidopsis resulted in enhanced tolerance to alkaline stress at the seedling and mature stages, but reduced ABA sensitivity. The closest Arabidopsis homolog mutant plants of Gshdz4, GsNAC019 and GsRD29B containing athb40, atnac019 and atrd29b were sensitive to alkaline stress. Overexpression or the closest Arabidopsis homolog mutant plants of the GsNAC019 gene in Arabidopsis positively or negatively regulated the expression of stress-related genes, such as AHA2, RD29A/B and KIN1. Moreover, this mutation could phenotypically promoted or compromised plant growth under alkaline stress, implying that GsNAC019 may contribute to alkaline stress

77 FR 42746 - Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting

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2012-07-20

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2012-N-0001] Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting AGENCY: Food and... of Committee: Advisory Committee for Pharmaceutical Science and Clinical Pharmacology. General...

75 FR 10488 - Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting

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2010-03-08

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-N-0001] Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting AGENCY: Food and... of Committee: Advisory Committee for Pharmaceutical Science and Clinical Pharmacology. General...

77 FR 1696 - Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting

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2012-01-11

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2012-N-0001] Advisory Committee for Pharmaceutical Science and Clinical Pharmacology; Notice of Meeting AGENCY: Food and... of Committee: Advisory Committee for Pharmaceutical Science and Clinical Pharmacology. General...

78 FR 61870 - Advisory Committee for Computer and Information Science and Engineering; Notice of Meeting

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2013-10-04

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Computer and Information Science and Engineering; Notice of Meeting In accordance with Federal Advisory Committee Act (Pub. L. 92-463, as amended... Committee for Computer and Information Science and Engineering (1115). Date/Time: Oct 31, 2013: 12:30 p.m...

75 FR 14578 - Federal Advisory Committee; Defense Science Board; Closed Meeting

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2010-03-26

... and homeland security. In accordance with section 10(d) of the Federal Advisory Committee Act, Public... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Science Board; Closed Meeting AGENCY: Department of Defense (DoD). ACTION: Notice of advisory committee meeting. SUMMARY...

77 FR 70483 - President's Committee on the National Medal of Science; Notice of Meeting

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2012-11-26

... NATIONAL SCIENCE FOUNDATION President's Committee on the National Medal of Science; Notice of... Science. Originally the meeting was scheduled for October 31, 2012. A notice was published in the Federal... Science Foundation announces the rescheduled meeting: Name: President's Committee on the National Medal of...

75 FR 64711 - Federal Advisory Committee; Defense Science Board

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2010-10-20

... Science Board before the meeting that is the subject of this notice. Due to internal DoD difficulties... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Science Board... Science Board will meet in closed session on October 27-28, 2010, at the Pentagon, Arlington, VA. FOR...

75 FR 61520 - President's Committee on the National Medal of Science; Notice of Meeting

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2010-10-05

... NATIONAL SCIENCE FOUNDATION President's Committee on the National Medal of Science; Notice of... Science Foundation announces the following meeting: Name: President's Committee on the National Medal of Science (1182). Date and Time: Wednesday, October 20, 2010, 8:30 a.m.-1:30 p.m. Place: Conference Room...

78 FR 42111 - Advisory Committee for Mathematical and Physical Sciences #66; Notice of Meeting; Correction

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2013-07-15

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences 66; Notice... July 18 Advisory Committee for Mathematical and Physical Sciences in the Federal Register on June 21..., Directorate for Mathematical and Physical Sciences, National Science Foundation, 4201 Wilson Blvd., Arlington...

76 FR 66997 - NASA Advisory Council; Technology and Innovation Committee; Meeting

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2011-10-28

... and Innovation Committee; Meeting ACTION: Notice of meeting. SUMMARY: In accordance with the Federal... (NASA) announces a meeting of the Technology and Innovation Committee of the NASA Advisory Council (NAC... Management Officer, National Aeronautics and Space Administration. [FR Doc. 2011-28005 Filed 10-27-11; 8:45...

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

Dr. Bhavya Lal, a researcher at the Institute for Defense Analysis's Science and Technology Policy Institute, testifies during a House Committee on Science, Space, and Technology hearing titled "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

78 FR 77502 - NASA Applied Sciences Advisory Committee Meeting

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2013-12-23

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (13-152)] NASA Applied Sciences Advisory... Aeronautics and Space Administration (NASA) announces a meeting of the Applied Sciences Advisory Committee.... ADDRESSES: NASA Headquarters, Room 3P40, 300 E Street SW., Washington, DC 20546. FOR FURTHER INFORMATION...

78 FR 57178 - NASA Applied Sciences Advisory Committee Meeting

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2013-09-17

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-115] NASA Applied Sciences Advisory... Aeronautics and Space Administration (NASA) announces a meeting of the Applied Sciences Advisory Committee.... ADDRESSES: NASA Headquarters, Room 1Q39, 300 E Street SW., Washington, DC 20546. FOR FURTHER INFORMATION...

78 FR 64255 - Committee on Equal Opportunities in Science and Engineering; Cancellation of Meeting

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2013-10-28

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering; Cancellation of Meeting SUMMARY: As a result of the impact of the recent government shutdown, the National... in Science and Engineering meeting. The public notice for this committee was published in the Federal...

76 FR 4138 - Committee on Equal Opportunity in Science and Engineering Solicitation of Recommendations for...

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2011-01-24

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunity in Science and Engineering Solicitation... recommendations for membership on the Committee on Equal Opportunities in Science and Engineering (CEOSE) (1173... provides advice to NSF on the implementation of the provisions of the Science and Engineering Equal...

77 FR 8275 - National Advisory Committee on Violence Against Women; Notice of Meeting

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2012-02-14

... DEPARTMENT OF JUSTICE National Advisory Committee on Violence Against Women; Notice of Meeting AGENCY: Office on Violence Against Women, United States Department of Justice. ACTION: Notice of Meeting... National Advisory Committee on Violence Against Women (hereinafter ``NAC''). DATES: The meeting will take...

76 FR 52971 - National Advisory Committee on Violence Against Women; Notice of Meeting

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2011-08-24

... DEPARTMENT OF JUSTICE National Advisory Committee on Violence Against Women; Notice of Meeting AGENCY: Office on Violence Against Women, United States Department of Justice. ACTION: Notice of Meeting... National Advisory Committee on Violence Against Women(hereinafter ``NAC''). DATES: The meeting will take...

78 FR 41115 - NASA Advisory Council; Science Committee; Meeting

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2013-07-09

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-074] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory... Time. ADDRESSES: NASA Headquarters, Room 7H45, 300 E Street SW., Washington, DC 20546. FOR FURTHER...

77 FR 1932 - Notification of a Public Teleconference of the Science Advisory Board Committee on Science...

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2012-01-12

... a draft report based on fact-finding activities conducted as part of a study of science integration... Science Advisory Board Committee on Science Integration for Decision Making AGENCY: Environmental Protection Agency (EPA). ACTION: Notice. SUMMARY: The Environmental Protection Agency (EPA or Agency) Science...

78 FR 32475 - Committee on Equal Opportunities in Science and Engineering; Notice of Meeting

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2013-05-30

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering; Notice of... Engineering (CEOSE) Advisory Committee Meeting, 1173. Dates/Time: June 19, 2013, 9:00 a.m.-5:30 p.m. June 20... participation in science and engineering. Agenda: Opening Statement by the CEOSE Chair [[Page 32476...

78 FR 17234 - Advisory Committee for Mathematical Sciences and Physical Sciences #66; Notice of Meeting

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2013-03-20

... Officer at the above address or the Web site at http://www.nsf.gov/mps/advisory.jsp . Purpose of Meeting... Opportunities in Science and Engineering, and the Advisory Committee for International Science and Engineering...

75 FR 31818 - National Science Board; Committee on Strategy and Budget; Sunshine Act Meetings; Notice

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2010-06-04

... NATIONAL SCIENCE FOUNDATION National Science Board; Committee on Strategy and Budget; Sunshine Act Meetings; Notice The National Science Board's Committee on Strategy and Budget, pursuant to NSF regulations (45 CFR part 614), the National Science Foundation Act, as amended (42 U.S.C. 1862n-5), and the...

76 FR 41824 - NASA Advisory Council; Science Committee; Meeting

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2011-07-15

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-068)] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory..., 2011, 7:30 a.m. to 11:30 a.m., Local Time. ADDRESSES: NASA Ames Research Center, NASA Ames Conference...

78 FR 20357 - NASA Advisory Council; Science Committee; Meeting

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2013-04-04

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 13-037] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory...:30 a.m. to 3:00 p.m., Local Time. ADDRESSES: NASA Headquarters, 300 E Street SW., Room 6H45...

77 FR 38093 - NASA Advisory Council; Science Committee; Meeting

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2012-06-26

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-046] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory.... to 2:30 p.m., local time. ADDRESSES: NASA Goddard Space Flight Center (GSFC), Building 1, Room E100E...

78 FR 67202 - NASA Advisory Council; Science Committee; Meeting

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2013-11-08

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 13-131] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory..., 2013, 8:30 a.m. to 3:00 p.m., Local Time. ADDRESSES: This meeting will take place at NASA Headquarters...

76 FR 17158 - NASA Advisory Council; Science Committee; Meeting

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2011-03-28

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-026)] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory....m. to 2 p.m., Local Time. ADDRESSES: NASA Headquarters, 300 E Street, SW., Room 5H45, Washington, DC...

75 FR 35091 - NASA Advisory Council; Science Committee; Meeting

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2010-06-21

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-068)] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory....m. to 1:30 p.m., e.d.t. ADDRESSES: NASA Headquarters, 300 E Street, SW., Room 3H46, Washington, DC...

77 FR 6824 - NASA Advisory Council; Science Committee; Meeting

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2012-02-09

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-010] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory....m. to 2 p.m., Local Time. ADDRESSES: NASA Headquarters, 300 E Street SW., Room 3H46 and 7H45...

76 FR 59446 - NASA Advisory Council; Science Committee; Meeting

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2011-09-26

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice11-084] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory..., 2011, 8:30 a.m. to 2 p.m., Local Time. ADDRESSES: NASA Headquarters, 300 E Street, SW., Room 3H46...

77 FR 3237 - Defense Science Board; Notice of Advisory Committee Meetings

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2012-01-23

.... national defense posture and homeland security. DATES: February 22-23, 2012. ADDRESSES: The Pentagon. FOR... DEPARTMENT OF DEFENSE Office of the Secretary Defense Science Board; Notice of Advisory Committee Meetings AGENCY: Department of Defense. ACTION: Notice of Advisory Committee Meetings. SUMMARY: The Defense...

77 FR 60717 - Establishment of the Advisory Committee on Climate Change and Natural Resource Science

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2012-10-04

... seeking nominations for the Advisory Committee on Climate Change and Natural Resource Science (Committee... of the U.S. Geological Survey National Climate Change and Wildlife Science Center and the DOI Climate... Partnership Coordinator, National Climate Change and Wildlife Science Center, U.S. Geological Survey, 12201...

75 FR 55617 - Advisory Committee for Biological Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-13

...-- Innovation Experiments; Research Resources. PM: Presentation and Discussion--Science, Arts and Humanities Symposium; COV Reports; COV Updates; New Ideas; Recognition of Departing BIO AC Members. October 7, 2010... NATIONAL SCIENCE FOUNDATION Advisory Committee for Biological Sciences; Notice of Meeting In...

76 FR 8380 - NASA Advisory Council; Science Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-14

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (11-114)] NASA Advisory Council; Science... Aeronautics and Space Administration (NASA) announces a meeting of the Science Committee of the NASA Advisory...:30 a.m. to 2 p.m., Local Time. ADDRESSES: NASA Headquarters, 300 E Street, SW., Rooms 9H40 and 3H46...

78 FR 69836 - Defense Science Board; Notice of Advisory Committee Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-21

..., tactics, and policies as they may affect the U.S. national defense posture and homeland security. In... DEPARTMENT OF DEFENSE Office of the Secretary Defense Science Board; Notice of Advisory Committee Meetings AGENCY: Department of Defense. ACTION: Notice of advisory committee meetings. SUMMARY: The Defense...

77 FR 60399 - Defense Science Board; Notice of Advisory Committee Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-03

.... national defense posture and homeland security. In accordance with section 10(d) of the Federal Advisory... DEPARTMENT OF DEFENSE Office of the Secretary Defense Science Board; Notice of Advisory Committee Meetings AGENCY: Department of Defense. ACTION: Notice of Advisory Committee Meetings. SUMMARY: The Defense...

78 FR 25971 - Defense Science Board; Notice of Advisory Committee Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

.... national defense posture and homeland security. In accordance with section 10(d) of the Federal Advisory... DEPARTMENT OF DEFENSE Office of the Secretary Defense Science Board; Notice of Advisory Committee Meetings AGENCY: Department of Defense. ACTION: Notice of Advisory Committee Meetings. SUMMARY: The Defense...

Molecular cloning and characterization of a membrane associated NAC family gene, SiNAC from foxtail millet [Setaria italica (L.) P. Beauv].

PubMed

Puranik, Swati; Bahadur, Ranjit Prasad; Srivastava, Prem S; Prasad, Manoj

2011-10-01

The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A transcript encoding NAC protein, termed SiNAC was identified from a salt stress subtractive cDNA library of S. italica seedling (Puranik et al., J Plant Physiol 168:280-287, 2011). This single/low copy gene containing four exons and four introns within the genomic-sequence encoded a protein of 462 amino acids. Structural analysis revealed that highly divergent C terminus contains a transmembrane domain. The NAC domain consisted of a twisted antiparallel beta-sheet packing against N terminal alpha helix on one side and a shorter helix on the other side. The domain was predicted to homodimerize and control DNA-binding specificity. The physicochemical features of the SiNAC homodimer interface justified the dimeric form of the predicted model. A 1539 bp fragment upstream to the start codon of SiNAC gene was cloned and in silico analysis revealed several putative cis-acting regulatory elements within the promoter sequence. Transactivation analysis indicated that SiNAC activated expression of reporter gene and the activation domain lied at the C terminal. The SiNAC:GFP was detected in the nucleus and cytoplasm while SiNAC ΔC(1-158):GFP was nuclear localized in onion epidermal cells. SiNAC transcripts mostly accumulated in young spikes and were strongly induced by dehydration, salinity, ethephon, and methyl jasmonate. These results suggest that SiNAC encodes a membrane associated NAC-domain protein that may function as a transcriptional activator in response to stress and developmental regulation in plants.

COMMITTEES: LISA 7 Science Organizing Committee and Local Organizing Committee LISA 7 Science Organizing Committee and Local Organizing Committee

NASA Astrophysics Data System (ADS)

2009-05-01

Science Organising Committee (SOC) Pierre Binetruy, APC - College de France Massimo Cerdonio, University of Padova Karsten Danzmann, AEI/University of Hannover Mike Cruise, University of Birmingham Jim Hough, University of Glasgow Oliver Jennrich, ESTEC Philippe Jetzer, University Zurich Alberto Lobo (Chair), ICE-CSIC and IEEC Yannick Mellier, IAP, Paris Bernard Schutz, AEI Potsdam Tim Sumner, Imperial College, London Jean-Yves Vinet, OCA, Nice Stefano Vitale, University of Trento Peter Bender, University of Colorado Sasha Buchman, Stanford University Joan Centrella, NASA/Goddard Neil Cornish, Montana State University Curt Cutler, NASA/JPL Sam Finn, Penn State University Jens Gundlach, NPL Craig Hogan, University of Washington Scott Hughes, MIT Piero Madau, Lick Observatory Tom Prince, NASA/JPL Sterl Phinney, Caltech Doug Richstone, University of Michigan Tuck Stebbins, NASA/Goddard Kip Thorne, Caltech Roger Blandford, Stanford University Eugenio Coccia, University of Roma-2 Carlos F Sopuerta,ICE-CSIC and IEEC Enrique Garcia-Berro, Universitat Politècnica de Catalunya, Barcelona Seiji Kawamura, National Observatory, Japan Jay Marx, LIGO Laboratory Stephen Merkowitz, NASA/Goddard Benoit Mours, Laboratoire d'Annec Gijs Nelemans, IMAPP, Nijmegen Enric Verdaguer, University of Barcelona Clifford M Will, Washington University, St Louis Local Organising Committee (LOC) Anna Bertolín (IEEC) Priscilla Cañizares (ICE-CSIC and IEEC) Carlos F Sopuerta (ICE-CSIC and IEEC) Ivan Lloro (ICE-CSIC and IEEC),Chair Alberto Lobo (ICE-CSIC and IEEC) Nacho Mateos (ICE-CSIC and IEEC) Pilar Montes (IEEC) Miquel Nofrarias (IEEC) Juan Ramos-Castro (Universitat Politècnica de Catalunya) Josep Sanjuán (IEEC)

Banana NAC transcription factor MusaNAC042 is positively associated with drought and salinity tolerance.

PubMed

Tak, Himanshu; Negi, Sanjana; Ganapathi, T R

2017-03-01

Banana is an important fruit crop and its yield is hampered by multiple abiotic stress conditions encountered during its growth. The NAC (NAM, ATAF, and CUC) transcription factors are involved in plant response to biotic and abiotic stresses. In the present study, we studied the induction of banana NAC042 transcription factor in drought and high salinity conditions and its overexpression in transgenic banana to improve drought and salinity tolerance. MusaNAC042 expression was positively associated with stress conditions like salinity and drought and it encoded a nuclear localized protein. Transgenic lines of banana cultivar Rasthali overexpressing MusaNAC042 were generated by Agrobacterium-mediated transformation of banana embryogenic cells and T-DNA insertion was confirmed by PCR and Southern blot analysis. Our results using leaf disc assay indicated that transgenic banana lines were able to tolerate drought and high salinity stress better than the control plants and retained higher level of total chlorophyll and lower level of MDA content (malondialdehyde). Transgenic lines analyzed for salinity (250 mM NaCl) and drought (Soil gravimetric water content 0.15) tolerance showed higher proline content, better Fv/Fm ratio, and lower levels of MDA content than control suggesting that MusaNAC042 may be involved in responses to higher salinity and drought stresses in banana. Expression of several abiotic stress-related genes like those coding for CBF/DREB, LEA, and WRKY factors was altered in transgenic lines indicating that MusaNAC042 is an efficient modulator of abiotic stress response in banana.

78 FR 79014 - Advisory Committee for Computer and Information Science and Engineering Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-27

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Computer and Information Science and Engineering Notice of Meeting In accordance with Federal Advisory Committee Act (Pub. L. 92-463, as amended... and Information Science and Engineering (1115) DATE/TIME: January 14, 2014, 3:00 p.m. to 5:00 p.m...

77 FR 26747 - Defense Science Board; Notice of Advisory Committee Meetings

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-07

... policies as they may affect the U.S. national defense posture and homeland security. DATES: May 23-24, 2012... DEPARTMENT OF DEFENSE Office of the Secretary Defense Science Board; Notice of Advisory Committee Meetings AGENCY: Department of Defense. ACTION: Notice of Advisory Committee Meetings. SUMMARY: The Defense...

The rose (Rosa hybrida) NAC transcription factor 3 gene, RhNAC3, involved in ABA signaling pathway both in rose and Arabidopsis.

PubMed

Jiang, Guimei; Jiang, Xinqiang; Lü, Peitao; Liu, Jitao; Gao, Junping; Zhang, Changqing

2014-01-01

Plant transcription factors involved in stress responses are generally classified by their involvement in either the abscisic acid (ABA)-dependent or the ABA-independent regulatory pathways. A stress-associated NAC gene from rose (Rosa hybrida), RhNAC3, was previously found to increase dehydration tolerance in both rose and Arabidopsis. However, the regulatory mechanism involved in RhNAC3 action is still not fully understood. In this study, we isolated and analyzed the upstream regulatory sequence of RhNAC3 and found many stress-related cis-elements to be present in the promoter, with five ABA-responsive element (ABRE) motifs being of particular interest. Characterization of Arabidopsis thaliana plants transformed with the putative RhNAC3 promoter sequence fused to the β-glucuronidase (GUS) reporter gene revealed that RhNAC3 is expressed at high basal levels in leaf guard cells and in vascular tissues. Moreover, the ABRE motifs in the RhNAC3 promoter were observed to have a cumulative effect on the transcriptional activity of this gene both in the presence and absence of exogenous ABA. Overexpression of RhNAC3 in A. thaliana resulted in ABA hypersensitivity during seed germination and promoted leaf closure after ABA or drought treatments. Additionally, the expression of 11 ABA-responsive genes was induced to a greater degree by dehydration in the transgenic plants overexpressing RhNAC3 than control lines transformed with the vector alone. Further analysis revealed that all these genes contain NAC binding cis-elements in their promoter regions, and RhNAC3 was found to partially bind to these putative NAC recognition sites. We further found that of 219 A. thaliana genes previously shown by microarray analysis to be regulated by heterologous overexpression RhNAC3, 85 are responsive to ABA. In rose, the expression of genes downstream of the ABA-signaling pathways was also repressed in RhNAC3-silenced petals. Taken together, we propose that the rose RhNAC3 protein

The Rose (Rosa hybrida) NAC Transcription Factor 3 Gene, RhNAC3, Involved in ABA Signaling Pathway Both in Rose and Arabidopsis

PubMed Central

Lü, Peitao; Liu, Jitao; Gao, Junping; Zhang, Changqing

2014-01-01

Plant transcription factors involved in stress responses are generally classified by their involvement in either the abscisic acid (ABA)-dependent or the ABA-independent regulatory pathways. A stress-associated NAC gene from rose (Rosa hybrida), RhNAC3, was previously found to increase dehydration tolerance in both rose and Arabidopsis. However, the regulatory mechanism involved in RhNAC3 action is still not fully understood. In this study, we isolated and analyzed the upstream regulatory sequence of RhNAC3 and found many stress-related cis-elements to be present in the promoter, with five ABA-responsive element (ABRE) motifs being of particular interest. Characterization of Arabidopsis thaliana plants transformed with the putative RhNAC3 promoter sequence fused to the β-glucuronidase (GUS) reporter gene revealed that RhNAC3 is expressed at high basal levels in leaf guard cells and in vascular tissues. Moreover, the ABRE motifs in the RhNAC3 promoter were observed to have a cumulative effect on the transcriptional activity of this gene both in the presence and absence of exogenous ABA. Overexpression of RhNAC3 in A. thaliana resulted in ABA hypersensitivity during seed germination and promoted leaf closure after ABA or drought treatments. Additionally, the expression of 11 ABA-responsive genes was induced to a greater degree by dehydration in the transgenic plants overexpressing RhNAC3 than control lines transformed with the vector alone. Further analysis revealed that all these genes contain NAC binding cis-elements in their promoter regions, and RhNAC3 was found to partially bind to these putative NAC recognition sites. We further found that of 219 A. thaliana genes previously shown by microarray analysis to be regulated by heterologous overexpression RhNAC3, 85 are responsive to ABA. In rose, the expression of genes downstream of the ABA-signaling pathways was also repressed in RhNAC3-silenced petals. Taken together, we propose that the rose RhNAC3 protein

76 FR 42714 - Science Board Advisory Committee; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-19

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0002] Science Board Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION... scientific evolutions in the fields of regulatory science, on formulating an appropriate research agenda, and...

76 FR 25359 - Science Board Advisory Committee; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-04

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0002] Science Board Advisory Committee; Notice of Meeting AGENCY: Food and Drug Administration, HHS. ACTION... scientific evolutions in the fields of regulatory science, on formulating an appropriate research agenda, and...

Analyses of the NAC transcription factor gene family in Gossypium raimondii Ulbr.: chromosomal location, structure, phylogeny, and expression patterns.

PubMed

Shang, Haihong; Li, Wei; Zou, Changsong; Yuan, Youlu

2013-07-01

NAC domain proteins are plant-specific transcription factors known to play diverse roles in various plant developmental processes. In the present study, we performed the first comprehensive study of the NAC gene family in Gossypium raimondii Ulbr., incorporating phylogenetic, chromosomal location, gene structure, conserved motif, and expression profiling analyses. We identified 145 NAC transcription factor (NAC-TF) genes that were phylogenetically clustered into 18 distinct subfamilies. Of these, 127 NAC-TF genes were distributed across the 13 chromosomes, 80 (55%) were preferentially retained duplicates located in both duplicated regions and six were located in triplicated chromosomal regions. The majority of NAC-TF genes showed temporal-, spatial-, and tissue-specific expression patterns based on transcriptomic and qRT-PCR analyses. However, the expression patterns of several duplicate genes were partially redundant, suggesting the occurrence of sub-functionalization during their evolution. Based on their genomic organization, we concluded that genomic duplications contributed significantly to the expansion of the NAC-TF gene family in G. raimondii. Comprehensive analysis of their expression profiles could provide novel insights into the functional divergence among members of the NAC gene family in G. raimondii. © 2013 Institute of Botany, Chinese Academy of Sciences.

75 FR 62891 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-13

... science and education activities within the Directorate for Mathematical and Physical Sciences. Agenda... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences; Notice of... Science Foundation announces the following meeting: Name: Directorate for Mathematical and Physical...

77 FR 64831 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-23

... education activities within the Directorate for Mathematical and Physical Sciences. Agenda: Update on... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences; Notice of... Science Foundation announces the following meeting: Name: Mathematical and Physical Sciences Advisory...

76 FR 64123 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-17

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences; Notice of... Science Foundation announces the following meeting: Name: Directorate for Mathematical and Physical.... Morris L. Aizenman, Senior Science Associate, Directorate for Mathematical and Physical Sciences, Room...

Barley plants over-expressing the NAC transcription factor gene HvNAC005 show stunting and delay in development combined with early senescence

PubMed Central

Christiansen, Michael W.; Matthewman, Colette; Podzimska-Sroka, Dagmara; O’Shea, Charlotte; Lindemose, Søren; Møllegaard, Niels Erik; Holme, Inger B.; Hebelstrup, Kim; Skriver, Karen; Gregersen, Per L.

2016-01-01

The plant-specific NAC transcription factors have attracted particular attention because of their involvement in stress responses, senescence, and nutrient remobilization. The HvNAC005 gene of barley encodes a protein belonging to subgroup NAC-a6 of the NAC family. This study shows that HvNAC005 is associated with developmental senescence. It was significantly up-regulated following ABA treatment, supported by ABA-responsive elements in its promoter, but it was not up-regulated during dark-induced senescence. The C-termini of proteins closely related to HvNAC005 showed overall high divergence but also contained conserved short motifs. A serine- and leucine-containing central motif was essential for transcriptional activity of the HvNAC005 C-terminus in yeast. Over-expression of HvNAC005 in barley resulted in a strong phenotype with delayed development combined with precocious senescence. The over-expressing plants showed up-regulation of genes involved with secondary metabolism, hormone metabolism, stress, signalling, development, and transport. Up-regulation of senescence markers and hormone metabolism and signalling genes supports a role of HvNAC005 in the cross field of different hormone and signalling pathways. Binding of HvNAC005 to promoter sequences of putative target genes containing the T[G/A]CGT core motif was shown by direct protein–DNA interactions of HvNAC005 with promoters for two of the up-regulated genes. In conclusion, HvNAC005 was shown to be a strong positive regulator of senescence and so is an obvious target for the fine-tuning of gene expression in future attempts to improve nutrient remobilization related to the senescence process in barley. PMID:27436280

75 FR 29369 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-25

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences; Notice of... Science Foundation announces the following meeting: Name: Directorate for Mathematical and Physical... Physical Sciences, Room 1005, National Science Foundation, 4201 Wilson Boulevard, Arlington, VA 22230. (703...

Genomic analysis of NAC transcription factors in banana (Musa acuminata) and definition of NAC orthologous groups for monocots and dicots.

PubMed

Cenci, Albero; Guignon, Valentin; Roux, Nicolas; Rouard, Mathieu

2014-05-01

Identifying the molecular mechanisms underlying tolerance to abiotic stresses is important in crop breeding. A comprehensive understanding of the gene families associated with drought tolerance is therefore highly relevant. NAC transcription factors form a large plant-specific gene family involved in the regulation of tissue development and responses to biotic and abiotic stresses. The main goal of this study was to set up a framework of orthologous groups determined by an expert sequence comparison of NAC genes from both monocots and dicots. In order to clarify the orthologous relationships among NAC genes of different species, we performed an in-depth comparative study of four divergent taxa, in dicots and monocots, whose genomes have already been completely sequenced: Arabidopsis thaliana, Vitis vinifera, Musa acuminata and Oryza sativa. Due to independent evolution, NAC copy number is highly variable in these plant genomes. Based on an expert NAC sequence comparison, we propose forty orthologous groups of NAC sequences that were probably derived from an ancestor gene present in the most recent common ancestor of dicots and monocots. These orthologous groups provide a curated resource for large-scale protein sequence annotation of NAC transcription factors. The established orthology relationships also provide a useful reference for NAC function studies in newly sequenced genomes such as M. acuminata and other plant species.

77 FR 42751 - National Advisory Committee Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Substance Abuse and Mental Health Services Administration... meeting on of the Substance Abuse and Mental Health Services Administration's (SAMHSA) National Advisory...://nac.samhsa.gov/ , or by contacting Geretta P. Wood. Committee Name: Substance Abuse and Mental Health...

77 FR 21812 - Biological Science Advisory Committee; Notice of Meeting: Correction

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-11

...The National Science Foundation (NSF) published in the Federal Register on April 2, 2012, a notice of an open meeting for the Biological Sciences Advisory Committee, 1110. This notice is to correct the year of the meeting date.

A Chemoenzymatic Histology Method for O-GlcNAc Detection.

PubMed

Aguilar, Aime Lopez; Hou, Xiaomeng; Wen, Liuqing; Wang, Peng G; Wu, Peng

2017-12-14

Modification of nuclear and cytoplasmic proteins by the addition or removal of O-GlcNAc dynamically impacts multiple biological processes. Here, we present the development of a chemoenzymatic histology method for the detection of O-GlcNAc in tissue specimens. We applied this method to screen murine organs, uncovering specific O-GlcNAc distribution patterns in different tissue structures. We then utilized our histology method for O-GlcNAc detection in human brain specimens from healthy donors and donors with Alzheimer's disease and found higher levels of O-GlcNAc in specimens from healthy donors. We also performed an analysis using a multiple cancer tissue array, uncovering different O-GlcNAc levels between healthy and cancerous tissues, as well as different O-GlcNAc cellular distributions within certain tissue specimens. This chemoenzymatic histology method therefore holds great potential for revealing the biology of O-GlcNAc in physiopathological processes. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

77 FR 42768 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-07-20

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences; Notice of... Science Foundation announces the following meeting: Name: Directorate for Mathematical and Physical... Person: Dr. Morris L. Aizenman, Senior Science Associate, Directorate for Mathematical and Physical...

75 FR 50783 - Committee for Social, Behavioral, and Economic Sciences Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-17

... NATIONAL SCIENCE FOUNDATION ADVISORY Committee for Social, Behavioral, and Economic Sciences..., Behavioral, and Economic Sciences ( 1171). Date/Time: September 7, 2010; 8:30 a.m. to 6 p.m. September 8... Assistant Director, Directorate for Social, Behavioral, and Economic Sciences, National Science Foundation...

77 FR 16076 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-19

... education activities within the Directorate for Mathematical and Physical Sciences. Agenda Update on current... National Science Foundation Advisory Committee for Mathematical and Physical Sciences; Notice of... Science Foundation announces the following meeting: Name: Directorate for Mathematical and Physical...

LISA 8 Science Organizing Committee and Local Organizing Committee LISA 8 Science Organizing Committee and Local Organizing Committee

NASA Astrophysics Data System (ADS)

2011-05-01

Science Organising Committee (SOC) Tom Abel, Stanford University Odylio Aguiar, Instituto Nacional de Pesquisas Espaciais Tal Alexander, Wizemann Institute Peter Bender, University of Colorado Pierre Binetruy, APC - College de France Sasha Buchman, Stanford University Robert Byer, Stanford University Manuela Campanelli, University of Texas Joan Centrella, NASA/Goddard Massimo Cerdonio, University of Padova Eugenio Coccia, University of Roma-2 Neil Cornish, Montana State University Michael Cruise, University of Birmingham Curt Cutler, NASA/JPL Karsten Danzmann, University of Hannover Sam Finn, Penn State University Jens Gundlach, NPL Gerhard Heinzel, Max-Planck-Institut fuer Gravitationsphysik Craig Hogan, University of Washington Jim Hough, University of Glasgow Scott Hughes, MIT Oliver Jennrich, ESTEC Philippe Jetzer, University Zurich Seiji Kawamura, National Observatory, Japan Alberto Lobo, ICE-CSIC and IEEC Avi Loeb, Harvard University Piero Madau, Lick Observatory Yannick Mellier, IAP, Paris Peter Michelson, Stanford University Guido Mueller, University of Florida Sterl Phinney, Caltech Tom Prince, NASA/JPL Doug Richstone, University of Michigan Bernard Schutz, AEI Potsdam Tuck Stebbins, NASA/Goddard Tim Sumner, Imperial College, London Ke-Xun Sun, Stanford University Kip Thorne, Caltech Michele Vallisneri, NASA/JPL Alberto Vecchio, University of Birmingham Jean-Yves Vinet, OCA, Nice Stefano Vitale, University of Trento Rai Weiss, MIT Nick White, NASA/Goddard Local Organising Committee (LOC) Sasha Buchman (Stanford University) Robert Byer (Stanford University) Sara Charbonneau-Lefort (Stanford University) Nancy Christianson (Stanford University) John Conklin (Stanford University) Dan DeBra (Stanford University) Jan Goebel (Stanford University) Vivian Drew (Stanford University) Ke-Xun Sun (Stanford University) Lucy Zhou (Stanford University) Andrea Zoellner (Stanford University)

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

Elizabeth Cantwell, chief executive officer at the Arizona State University Research Enterprise, testifies during a House Committee on Science, Space, and Technology hearing titled "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

Rep. Lamar Smith, R-Texas, chairman of the House Committee on Science, Space, and Technology, delivers his opening statement during a hearing titled "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

78 FR 24241 - Nanoscale Science, Engineering, and Technology Subcommittee; Committee on Technology, National...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-24

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering, and Technology Subcommittee; Committee on Technology, National Science and Technology Council; Notice of Public Meeting AGENCY: Executive Office of the President, Office of Science and Technology Policy. ACTION: Notice of Public Meeting...

77 FR 61448 - Nanoscale Science, Engineering and Technology Subcommittee Committee on Technology, National...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-09

... OFFICE OF SCIENCE AND TECHNOLOGY POLICY Nanoscale Science, Engineering and Technology Subcommittee Committee on Technology, National Science and Technology Council; Public Meetings AGENCY: Executive Office of the President, Office of Science and Technology Policy. ACTION: Notice of Public Meetings. SUMMARY...

78 FR 50085 - Advisory Committee on Climate Change and Natural Resource Science

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-16

... Climate Change and Natural Resource Science AGENCY: U.S. Geological Survey, Interior. ACTION: Meeting.... 2, we announce that the Advisory Committee on Climate Change and Natural Resource Science will hold... Partnership Coordinator, National Climate Change and Wildlife Science Center, U.S. Geological Survey, 12201...

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

NASA Associate Administrator for the Human Exploration and Operations Mission Directorate William Gerstenmaier testifies during a House Committee on Science, Space, and Technology hearing titled "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

Rep. Eddie Bernice Johnson, D-Texas, ranking member of the House Committee on Science, Space, and Technology delivers her opening statement during a hearing titled "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

77 FR 30029 - Advisory Committee for International Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-21

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering (25104). Date and Time: June 11, 2012, 10:00 a.m.-12:00 p.m. Place.... Contact Person for More Information: Robert Webber, Office of International Science and Engineering...

78 FR 13384 - Advisory Committee for International Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-27

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering (25104). Date/Time: March 14, 2013 9:30 a.m.-5:00 p.m. March 15, 2013 8:30 a.m.-12... of International Science and Engineering, National Science Foundation, 4201 Wilson Blvd., Arlington...

76 FR 20051 - Advisory Committee for Computer and Information; Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-11

... Engineering; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as... Committee for Computer and Information Science and Engineering--(1115). Date and Time: May 6, 2011 8:30 a.m... Engineering, National Science Foundation, 4201 Wilson Blvd., Suite 1105, Arlington, VA 22230. Telephone: (703...

75 FR 55616 - NASA Advisory Council; Information Technology Infrastructure Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-13

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (10-110)] NASA Advisory Council...-463, as amended, the National Aeronautics and Space Administration (NASA) announce a meeting for the Information Technology Infrastructure Committee of the NASA Advisory Council (NAC). DATES: Tuesday, September...

The Nitrate-Inducible NAC Transcription Factor TaNAC2-5A Controls Nitrate Response and Increases Wheat Yield1[OPEN

PubMed Central

He, Xue; Qu, Baoyuan; Li, Wenjing; Zhao, Xueqiang; Teng, Wan; Ma, Wenying; Ren, Yongzhe; Li, Bin; Li, Zhensheng; Tong, Yiping

2015-01-01

Nitrate is a major nitrogen resource for cereal crops; thus, understanding nitrate signaling in cereal crops is valuable for engineering crops with improved nitrogen use efficiency. Although several regulators have been identified in nitrate sensing and signaling in Arabidopsis (Arabidopsis thaliana), the equivalent information in cereals is missing. Here, we isolated a nitrate-inducible and cereal-specific NAM, ATAF, and CUC (NAC) transcription factor, TaNAC2-5A, from wheat (Triticum aestivum). A chromatin immunoprecipitation assay showed that TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase. Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate and, hence, increased the root’s ability to acquire nitrogen. Furthermore, we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts and allocated more nitrogen in grains in a field experiment. These results suggest that TaNAC2-5A is involved in nitrate signaling and show that it is an exciting gene resource for breeding crops with more efficient use of fertilizer. PMID:26371233

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

NASA Associate Administrator for the Human Exploration and Operations Mission Directorate William Gerstenmaier is seen prior to the start of a House Committee on Science, Space, and Technology Hearing titles "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

Associations and Committees of or for Women in Science, Engineering, Mathematics and Medicine.

ERIC Educational Resources Information Center

Aldrich, Michele, Comp.; Leach, Alicia, Comp.

Provided is a list of associations and committees of or for women in science, engineering, mathematics, and medicine. The list is organized by discipline, with cross-referencing to cognate specialties. The disciplines include: anthropology; astronomy; atmospheric sciences; biology; chemistry; computer sciences; earth sciences; energy; engineering;…

78 FR 70963 - NASA Advisory Council; Technology and Innovation Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-27

... Technology Mission Directorate programs with an emphasis on Solar Electric Propulsion and Cryogenic... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: 13-137] NASA Advisory Council; Technology... of the Technology and Innovation Committee (TIC) of the NASA Advisory Council (NAC). The meeting will...

78 FR 37590 - Advisory Committee for Mathematical and Physical Sciences #66; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-21

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Mathematical and Physical Sciences 66; Notice... Physical Sciences ( 66). Dates/Time: July 18, 2013 1:00 p.m.-5:15 p.m. Place: National Science Foundation... Federal Officer, Directorate for Mathematical and Physical Sciences, National Science Foundation, 4201...

Targeting Protein O-GlcNAc Modifications In Breast Cancer

DTIC Science & Technology

2010-09-30

O-GlcNAcation and elevated expression of O-GlcNAc transferase (OGT), the enzyme catalyzing addition of O-GlcNAc to proteins. Reduction of O...regulatory switch mechanism analogous to phosphorylation (28). Cytosolic and nuclear enzymes dynamically catalyze addition (O-GlcNAc transferase or OGT) and...levels, through pharmacological inhibition or genetic knock-down of enzymes that add or remove O-GlcNAc, can inhibit ErbB2-mediated oncogenic

75 FR 52996 - Advisory Committee for International Science & Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-30

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science & Engineering; Notice of... Engineering ( 25104). Date/Time: September 20, 2010; 9 a.m. to 5:15 p.m.; September 21, 2009; 8:30 a.m. to 12..., education and related activities involving U.S. science and engineering working within a global context, as...

76 FR 14996 - Advisory Committee for Mathematical and Physical Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-18

.... Aizenman, Senior Science Associate, Directorate for Mathematical and Physical Sciences, Room 1005, National... Committee of Visitors Report of NSF Advisory Working Groups Meeting of MPSAC with Divisions within MPS...

77 FR 13367 - Advisory Committee for International Science and Engineering; Notice of Meeting

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2012-03-06

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering (25104). Date and Time: March 19, 2012, 8:30 a.m.-5 p.m. March 20, 2012, 8:30 a.m.... Type of Meeting: Open. Contact Person: Robert Webber, Office of International Science and Engineering...

77 FR 61644 - Advisory Committee for International Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-10

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering (25104). Date and Time: October 25, 2012 8:30 a.m.-5 p.m. October 26, 2012 8:30 a...: Open. Contact Person: Robert Webber, NSF Office of International Science and Engineering, 4201 Wilson...

[ManNAc, a new therapeutic agent to reduce Angptl4-induced proteinuria in MCD].

PubMed

Clément, Lionel; Macé, Camille

2016-01-01

Current therapies used in minimal change disease (MCD) were originally designed to cure other diseases. They are only partially efficient, and present inconvenient side effects. Therefore, understanding the molecular mechanisms implicated in the pathogenesis of proteinuria in MCD could lead to new therapeutic strategies. A new experimental transgenic rat model of human MCD was generated. These NPHS2-Angptl4 transgenic rats over-express two different forms of the glycoprotein Angptl4 from the podocyte. The majority of the protein shows a lack of sialylation that is implicated in the pathogenesis of proteinuria. Supplementation of ManNAc, a precursor of sialic acid, significantly reduces albuminuria in those rats by increasing sialylation of the hyposialylated form of Angptl4. After treatment of the first episode of MCD with glucocorticoids in patients, ManNAc could be used as a maintenance drug, especially to reduce the frequency and intensity of relapse. ManNAc is a promising therapeutic agent for patients with MCD. © 2016 médecine/sciences – Inserm.

77 FR 62538 - Advisory Committee for Social, Behavioral and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-15

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral and Economic Sciences..., Behavioral and Economic Sciences ( 1171). Date/Time: November 15, 2012; 8:00 a.m. to 5:00 p.m. Place..., Behavioral and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard, Room 905, Arlington...

77 FR 38092 - NASA Advisory Council; Information Technology Infrastructure Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-26

.... The meeting will be held for the purpose of soliciting from the information technology community and... NAC Information Technology Infrastructure Committee meeting in Building 28. All U.S. citizens and... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-048] NASA Advisory Council; Information...

77 FR 38678 - NASA Advisory Council; Technology and Innovation Committee; Meeting

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2012-06-28

... and Innovation Committee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION... National Aeronautics and Space Administration (NASA) announces a meeting of the Technology and Innovation... access badge to enter GSFC and must state that they are attending the NAC's Technology and Innovation...

77 FR 64561 - NASA Advisory Council; Technology and Innovation Committee; Meeting

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2012-10-22

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-083] NASA Advisory Council; Technology...: Notice of meeting. SUMMARY: The National Aeronautics and Space Administration (NASA) announces a meeting of the Technology and Innovation Committee of the NASA Advisory Council (NAC). The meeting will be...

76 FR 22100 - Notification of a Public Teleconference of the Science Advisory Board; Drinking Water Committee...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-20

... Science Advisory Board; Drinking Water Committee Augmented for the Review of the Effectiveness of Partial... Drinking Water Committee Augmented for the Review of the Effectiveness of Partial Lead Service Line... EPA Science Advisory Board can be found on the EPA Web site at http://www.epa.gov/sab . SUPPLEMENTARY...

76 FR 65219 - Advisory Committee for Social, Behavioral and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-20

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral and Economic Sciences..., Behavioral and Economic Sciences ( 1171) Date/Time: November 3, 2011; 1 p.m. to 5:30 p.m. November 4, 2011; 8..., Directorate for Social, Behavioral and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

78 FR 60918 - Committee on Equal Opportunities in Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-02

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering; Notice of... Engineering (1173). Dates/Time: October 30, 2013, 10:00 a.m.-3:30 p.m. Place: National Science Foundation... advice and recommendations concerning broadening participation in science and engineering. Agenda...

House Committee on Science, Space, and Technology Hearing

NASA Image and Video Library

2018-05-17

NASA Associate Administrator for the Human Exploration and Operations Mission Directorate William Gerstenmaier, left, Dr. Bhavya Lal, a researcher at the Institute for Defense Analysis's Science and Technology Policy Institute, center, and Dr. Elizabeth Cantwell, chief executive officer at the Arizona State University Research Enterprise, right, listen as Rep. Brian Babin, R-Texas, is seen on screen as he delivers an opening statement during a House Committee on Science, Space, and Technology hearing titled "America's Human Presence in Low-Earth Orbit" on Thursday, May 17, 2018 in the Rayburn House Office Building in Washington. Photo Credit: (NASA/Joel Kowsky)

76 FR 24062 - Advisory Committee for Social, Behavioral and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-29

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral and Economic Sciences..., Behavioral and Economic Sciences ( 1171) Date/Time: May 19, 2011; 9 a.m. to 5:30 p.m. May 20, 2011; 9 a.m. to..., Directorate for Social, Behavioral and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

77 FR 25207 - Advisory Committee for Social, Behavioral and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-27

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral and Economic Sciences..., Behavioral and Economic Sciences ( 1171). Date/Time: May 17, 2012; 8:30 a.m. to 5:30 p.m.; May 18, 2012; 8:30..., Directorate for Social, Behavioral and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

75 FR 25886 - Advisory Committee for Social, Behavioral, and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-10

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Social, Behavioral, and Economic Sciences..., Behavioral, and Economic Sciences ( 1171). Date/Time: May 20, 2010; 8:30 a.m. to 5:30 p.m. May 21, 2010; 8:30..., Directorate for Social, Behavioral, and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

75 FR 4110 - NASA Advisory Council; Technology and Innovation Committee; Meeting.

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-26

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice (10-010)] NASA Advisory Council; Technology... amended, the National Aeronautics and Space Administration (NASA) announce a meeting of the newly formed Technology and Innovation Committee of the NASA Advisory Council (NAC). This will be the first meeting of...

77 FR 67028 - NASA Advisory Council; Information Technology Infrastructure Committee; Meeting

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2012-11-08

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice 12-092] NASA Advisory Council; Information... Technology Infrastructure Committee (ITIC) of the NASA Advisory Council (NAC). DATES: Tuesday, November 27, 2012, 1:00 to 5:00 p.m., Local Time. ADDRESSES: NASA Marshall Space Flight Center, Building 4200, Room...

78 FR 5860 - Eighth Meeting: RTCA Next Gen Advisory Committee (NAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-28

...] Preliminary report to identify and mitigate barriers to implementing PBN. Issues associated with Implementing RNAV/RNP [cir] An open discussion by the Committee of Non-technical barriers of PBN procedures... NextGen. Cat Ex 2 Task Group [cir] Preliminary report on the recommendation for implementing new...

75 FR 13313 - Advisory Committee for International Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... NATIONAL SCIENCE FOUNDATION Advisory Committee for International Science and Engineering; Notice... Science and Engineering ( 25104). Date/Time: April 19, 2010--8:30 a.m. to 5 p.m. April 20, 2010--8:30 a.m... Engineering. Agenda April 19, 2010 AM: Introductions and Updates--Presentation and Discussion of 2010...

Sequential Desorption of Nitroaromatic Compounds (NAC) from Soils

DTIC Science & Technology

2005-03-01

the soil solution . Weissmahr et al. [20] suggest an electron donor acceptor (EDA) complex between oxygens of the siloxane surface of the clays and the...release of NACs into the soil solution . At high pH values desorption is superimposed by NACs hydrolysis. Therefore, in- creasing pH values impedes the...presented demonstrate that both the cation present in the soil solution and its concentration may affect the desorption behavior of NACs in contaminated soils

Nutrient-driven O-GlcNAc in proteostasis and neurodegeneration.

PubMed

Akan, Ilhan; Olivier-Van Stichelen, Stephanie; Bond, Michelle R; Hanover, John A

2018-01-01

Proteostasis is essential in the mammalian brain where post-mitotic cells must function for decades to maintain synaptic contacts and memory. The brain is dependent on glucose and other metabolites for proper function and is spared from metabolic deficits even during starvation. In this review, we outline how the nutrient-sensitive nucleocytoplasmic post-translational modification O-linked N-acetylglucosamine (O-GlcNAc) regulates protein homeostasis. The O-GlcNAc modification is highly abundant in the mammalian brain and has been linked to proteopathies, including neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's. C. elegans, Drosophila, and mouse models harboring O-GlcNAc transferase- and O-GlcNAcase-knockout alleles have helped define the role O-GlcNAc plays in development as well as age-associated neurodegenerative disease. These enzymes add and remove the single monosaccharide from protein serine and threonine residues, respectively. Blocking O-GlcNAc cycling is detrimental to mammalian brain development and interferes with neurogenesis, neural migration, and proteostasis. Findings in C. elegans and Drosophila model systems indicate that the dynamic turnover of O-GlcNAc is critical for maintaining levels of key transcriptional regulators responsible for neurodevelopment cell fate decisions. In addition, pathways of autophagy and proteasomal degradation depend on a transcriptional network that is also reliant on O-GlcNAc cycling. Like the quality control system in the endoplasmic reticulum which uses a 'mannose timer' to monitor protein folding, we propose that cytoplasmic proteostasis relies on an 'O-GlcNAc timer' to help regulate the lifetime and fate of nuclear and cytoplasmic proteins. O-GlcNAc-dependent developmental alterations impact metabolism and growth of the developing mouse embryo and persist into adulthood. Brain-selective knockout mouse models will be an important tool for understanding the role of O-GlcNAc in the

78 FR 25309 - Advisory Committee for Social, Behavioral and Economic Sciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-30

... NATIONAL SCIENCE FOUNDATION ADVISORY Advisory Committee for Social, Behavioral and Economic..., Behavioral and Economic Sciences ( 1171) Date/Time: May 20, 2013; 9:00 a.m. to 5:15 p.m. May 21, 2013; 9:00 a..., Directorate for Social, Behavioral and Economic Sciences, National Science Foundation, 4201 Wilson Boulevard...

76 FR 4138 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: February 8, 2011, 9 a.m.-5:30 p.m. February 9, 2011... National Science Foundation (NSF) concerning broadening participation in science and engineering. Agenda...

75 FR 62591 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-12

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Equal Opportunities in Science and Engineering (1173). Dates/Time: October 25, 2010, 8:30 a.m.-5:30 p.m... the National Science Foundation (NSF) concerning broadening participation in science and engineering...

MRI to predict nipple-areola complex (NAC) involvement: An automatic method to compute the 3D distance between the NAC and tumor.

PubMed

Giannini, Valentina; Bianchi, Veronica; Carabalona, Silvia; Mazzetti, Simone; Maggiorotto, Furio; Kubatzki, Franziska; Regge, Daniele; Ponzone, Riccardo; Martincich, Laura

2017-12-01

To assess the role in predicting nipple-areola complex (NAC) involvement of a newly developed automatic method which computes the 3D tumor-NAC distance. Ninety-nine patients scheduled to nipple sparing mastectomy (NSM) underwent magnetic resonance (MR) examination at 1.5 T, including sagittal T2w and dynamic contrast enhanced (DCE)-MR imaging. An automatic method was developed to segment the NAC and the tumor and to compute the 3D distance between them. The automatic measurement was compared with manual axial and sagittal 2D measurements. NAC involvement was defined by the presence of invasive ductal or lobular carcinoma and/or ductal carcinoma in situ or ductal intraepithelial neoplasia (DIN1c - DIN3). Tumor-NAC distance was computed on 95/99 patients (25 NAC+), as three tumors were not correctly segmented (sensitivity = 97%), and 1 NAC was not detected (sensitivity = 99%). The automatic 3D distance reached the highest area under the receiver operating characteristic (ROC) curve (0.830) with respect to the manual axial (0.676), sagittal (0.664), and minimum distances (0.664). At the best cut-off point of 21 mm, the 3D distance obtained sensitivity = 72%, specificity = 80%, positive predictive value = 56%, and negative predictive value = 89%. This method could provide a reproducible biomarker to preoperatively select breast cancer patients candidates to NSM, thus helping surgical planning and intraoperative management of patients. © 2017 Wiley Periodicals, Inc.

Isolation and Expression of NAC Genes during Persimmon Fruit Postharvest Astringency Removal

PubMed Central

Min, Ting; Wang, Miao-Miao; Wang, Hongxun; Liu, Xiaofen; Fang, Fang; Grierson, Donald; Yin, Xue-Ren; Chen, Kun-Song

2015-01-01

NAC genes have been characterized in numerous plants, where they are involved in responses to biotic and abiotic stress, including low oxygen stress. High concentration of CO2 is one of the most effective treatments to remove astringency of persimmon fruit owing to the action of the accumulated anoxia metabolite acetaldehyde. In model plants, NAC genes have been identified as being responsive to low oxygen. However, the possible relationship between NAC transcription factors and persimmon astringency removal remains unexplored. In the present research, treatment with a high concentration of CO2 (95%) effectively removed astringency of “Mopan” persimmon fruit by causing decreases in soluble tannin. Acetaldehyde content increased in response to CO2 treatment concomitantly with astringency removal. Using RNA-seq and Rapid amplification of cDNA ends (RACE), six DkNAC genes were isolated and studied. Transcriptional analysis indicated DkNAC genes responded differentially to CO2 treatment; DkNAC1, DkNAC3, DkNAC5 and DkNAC6 were transiently up-regulated, DkNAC2 was abundantly expressed 3 days after treatment, while the DkNAC4 was suppressed during astringency removal. It is proposed that DkNAC1/3/5/6 could be important candidates as regulators of persimmon astringency removal and the roles of other member are also discussed. PMID:25599529

OGA inhibition by GlcNAc-selenazoline

PubMed Central

Kim, Eun Ju; Love, Dona C.; Darout, Etzer; Abdo, Mohannad; Rempel, Brian; Withers, Stephen G.; Rablen, Paul R.; Hanover, John A.; Knapp, Spencer

2010-01-01

The title compound, which differs from the powerful O-GlcNAcase (OGA) inhibitor GlcNAc-thiazoline only at the chalcogen atom (Se for S), is a much weaker inhibitor in a direct OGA assay. In human cells, however, the selenazoline shows comparable ability to induce hyper-O-GlcNAc-ylation, and the two show similar reduction of insulin-stimulated translocation of glucose transporter 4 in differentiated 3T3 adipocytes. PMID:20822912

A new tomato NAC (NAM/ATAF1/2/CUC2) transcription factor, SlNAC4, functions as a positive regulator of fruit ripening and carotenoid accumulation.

PubMed

Zhu, Mingku; Chen, Guoping; Zhou, Shuang; Tu, Yun; Wang, Yi; Dong, Tingting; Hu, Zongli

2014-01-01

Fruit ripening in tomato (Solanum lycopersicum) is a complicated development process affected by both endogenous hormonal and genetic regulators and external signals. Although the role of NOR, a member of the NAC domain family, in mediating tomato fruit ripening has been established, its underlying molecular mechanisms remain unclear. To explore further the role of NAC transcription factors in fruit ripening, we characterized a new tomato NAC domain protein, named SlNAC4, which shows high accumulation in sepal and at the onset of fruit ripening. Various stress treatments including wounding, NaCl, dehydration and low temperature significantly increased the expression of SlNAC4. Reduced expression of SlNAC4 by RNA interference (RNAi) in tomato resulted in delayed fruit ripening, suppressed Chl breakdown and decreased ethylene synthesis mediated mainly through reduced expression of ethylene biosynthesis genes of system-2, and reduced carotenoids by alteration of the carotenoid pathway flux. Transgenic tomato fruits also displayed significant down-regulation of multiple ripening-associated genes, indicating that SlNAC4 functions as a positive regulator of fruit ripening by affecting ethylene synthesis and carotenoid accumulation. Moreover, we also noted that SlNAC4 could not be induced by ethylene and may function upstream of the ripening regulator RIN and positively regulate its expression. Yeast two-hybrid assay further revealed that SlNAC4 could interact with both RIN and NOR protein. These results suggested that ethylene-dependent and -independent processes are regulated by SlNAC4 in the fruit ripening regulatory network.

Proteolysis of HCF-1 by Ser/Thr glycosylation-incompetent O-GlcNAc transferase:UDP-GlcNAc complexes

PubMed Central

Kapuria, Vaibhav; Röhrig, Ute F.; Bhuiyan, Tanja; Borodkin, Vladimir S.; van Aalten, Daan M.F.; Zoete, Vincent; Herr, Winship

2016-01-01

In complex with the cosubstrate UDP-N-acetylglucosamine (UDP-GlcNAc), O-linked-GlcNAc transferase (OGT) catalyzes Ser/Thr O-GlcNAcylation of many cellular proteins and proteolysis of the transcriptional coregulator HCF-1. Such a dual glycosyltransferase–protease activity, which occurs in the same active site, is unprecedented and integrates both reversible and irreversible forms of protein post-translational modification within one enzyme. Although occurring within the same active site, we show here that glycosylation and proteolysis occur through separable mechanisms. OGT consists of tetratricopeptide repeat (TPR) and catalytic domains, which, together with UDP-GlcNAc, are required for both glycosylation and proteolysis. Nevertheless, a specific TPR domain contact with the HCF-1 substrate is critical for proteolysis but not Ser/Thr glycosylation. In contrast, key catalytic domain residues and even a UDP-GlcNAc oxygen important for Ser/Thr glycosylation are irrelevant for proteolysis. Thus, from a dual glycosyltransferase–protease, essentially single-activity enzymes can be engineered both in vitro and in vivo. Curiously, whereas OGT-mediated HCF-1 proteolysis is limited to vertebrate species, invertebrate OGTs can cleave human HCF-1. We present a model for the evolution of HCF-1 proteolysis by OGT. PMID:27056667

Notification: EPA’s Use of Science and Research Federal Advisory Committees to Improve Agency Performance

EPA Pesticide Factsheets

Project #OPE-FY16-0024, May 9, 2016.The EPA OIG plans to begin preliminary research on internal controls the EPA has in place to engage science and research Federal Advisory Committee Act (FACA) committees and utilize their recommendations.

O-GlcNAc: a novel regulator of immunometabolism.

PubMed

Machacek, Miranda; Slawson, Chad; Fields, Patrick E

2018-06-01

The rapidly expanding field of immunometabolism focuses on how metabolism controls the function of immune cells. CD4 + T cells are essential for the adaptive immune response leading to the eradication of specific pathogens. However, when T cells are inappropriately over-active, they can drive autoimmunity, allergic disease, and chronic inflammation. The mechanisms by which metabolic changes influence function in CD4 + T cells are not fully understood. The post-translational protein modification, O-GlcNAc (O-linked β-N-acetylglucosamine), dynamically cycles on and off of intracellular proteins as cells respond to their environment and flux through metabolic pathways changes. As the rate of O-GlcNAc cycling fluctuates, protein function, stability, and/or localization can be affected. Thus, O-GlcNAc is critically poised at the nexus of cellular metabolism and function. This review highlights the intra- and extracellular metabolic factors that influence CD4 + T cell activation and differentiation and how O-GlcNAc regulates these processes. We also propose areas of future research that may illuminate O-GlcNAc's role in the plasticity and pathogenicity of CD4 + T cells and uncover new potential therapeutic targets.

77 FR 6143 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-07

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: February 28, 2012, 9 a.m.-5:30 p.m., February 29... provide advice and recommendations concerning broadening participation in science and engineering. Agenda...

75 FR 61778 - NASA Advisory Council; Science Committee; Astrophysics Subcommittee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-06

... NATIONAL AERONAUTICS AND SPACE ADMINISTRATION [Notice: (10-118)] NASA Advisory Council; Science Committee; Astrophysics Subcommittee; Meeting AGENCY: National Aeronautics and Space Administration. ACTION... amended, the National Aeronautics and Space Administration (NASA) announces a meeting of the Astrophysics...

77 FR 61790 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-11

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: October 30, 2012, 10 a.m.-5:30 p.m.; October 31... science and engineering. Agenda Tuesday, October 30, 2012 Opening Statement by the CEOSE Chair...

78 FR 8596 - Committee on Equal Opportunities in Science and Engineering #1173; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-06

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering 1173... Science and Engineering (CEOSE). Dates/Time: February 25, 2013, 9:00 a.m.-5:30 p.m.; February 26, 2013, 9... participation in science and engineering. Agenda: Opening Statement by the CEOSE Chair Discussions: Concurrence...

77 FR 31893 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-30

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173) Dates/Time: June 19, 2012, 1:00 p.m.-6:00 p.m.; June 20, 2012, 9... advice and recommendations concerning broadening participation in science and engineering. Agenda Tuesday...

75 FR 6063 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-02-05

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: March 8, 2010, 8:30 a.m.-5:30 p.m.; March 9, 2010... concerning broadening participation in science and engineering. Agenda Primary Focus of This Meeting...

The Wheat NAC Transcription Factor TaNAC2L Is Regulated at the Transcriptional and Post-Translational Levels and Promotes Heat Stress Tolerance in Transgenic Arabidopsis.

PubMed

Guo, Weiwei; Zhang, Jinxia; Zhang, Ning; Xin, Mingming; Peng, Huiru; Hu, Zhaorong; Ni, Zhongfu; Du, Jinkun

2015-01-01

Heat stress poses a serious threat to global crop production. In efforts that aim to mitigate the adverse effects of heat stress on crops, a variety of genetic tools are being used to develop plants with improved thermotolerance. The characterization of important regulators of heat stress tolerance provides essential information for this aim. In this study, we examine the wheat (Triticum aestivum) NAC transcription factor gene TaNAC2L. High temperature induced TaNAC2L expression in wheat and overexpression of TaNAC2L in Arabidopsis thaliana enhanced acquired heat tolerance without causing obvious alterations in phenotype compared with wild type under normal conditions. TaNAC2L overexpression also activated the expression of heat-related genes in the transgenic Arabidopsis plants, suggesting that TaNAC2L may improve heat tolerance by regulating the expression of stress-responsive genes. Notably, TaNAC2L is also regulated at the post-translational level and might be degraded via a proteasome-mediated pathway. Thus, this wheat transcription factor may have potential uses in enhancing thermotolerance in crops.

75 FR 33652 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-14

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: June 29, 2010, 8:30 p.m.-5:30 p.m. June 30, 2010, 8... NSF concerning broadening participation in science and engineering. Agenda Monday, June 29, 2010...

76 FR 55951 - Committee on Equal Opportunities in Science and Engineering (CEOSE); Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-09

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering (CEOSE... Opportunities in Science and Engineering (1173). Dates/Time: October 17, 2011, 9 a.m.-5:30 p.m. October 18, 2011... science and engineering. Agenda: Monday, Oct 17, 2011 Opening Statement by the CEOSE Chair Presentations...

Testimony to the House Science Space and Technology Committee.

DOE Office of Scientific and Technical Information (OSTI.GOV)

Church, Michael Kenton; Tannenbaum, Benn

Chairman Smith, Ranking Member Johnson, and distinguished members of the Committee on Science, Space, and Technology, I thank you for the opportunity to testify today on the role of science, engineering, and research at Sandia National Laboratories, one of the nation’s premiere national labs and the nation’s largest Federally Funded Research and Development Center (FFRDC) laboratory. I am Dr. Susan Seestrom, Sandia’s Associate Laboratories Director for Advanced Science & Technology (AST) and Chief Research Officer (CRO). As CRO I am responsible for research strategy, Laboratory Directed Research & Development (LDRD), partnerships strategy, and technology transfer. As director and line managermore » for AST I manage capabilities and mission delivery across a variety of the physical and mathematical sciences and engineering disciplines, such as pulsed power, radiation effects, major environmental testing, high performance computing, and modeling and simulation.« less

77 FR 56662 - Homeland Security Science and Technology Advisory Committee (HSSTAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-13

... as new developments in systems engineering, cyber-security, knowledge management and how best to... Management; Notice of Federal Advisory Committee Meeting. SUMMARY: The Homeland Security Science and... the Under Secretary on policies, management processes, and organizational constructs as needed. Agenda...

78 FR 66949 - Homeland Security Science and Technology Advisory Committee (HSSTAC)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-07

... Technology, such as new developments in systems engineering, cyber-security, knowledge management and how... Management; Notice of Federal Advisory Committee Meeting. SUMMARY: The Homeland Security Science and... also advises the Under Secretary on policies, management processes, and organizational constructs as...

75 FR 37452 - Science Board Advisory Committee; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-29

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-N-0001... Safety and Applied Nutrition (CFSAN). The Science Board will hear about an interim report from the... the committee. Written submissions may be made to the contact person on or before August 9, 2010. Oral...

The NACS Education Program

ERIC Educational Resources Information Center

Thodt, Charles A.

1978-01-01

The National Association of College Stores has for 31 years offered intensive formal training in bookstore management. The NACS Management Survey/Management Seminar, Booksellers School, and Advanced Seminars on Personnel Supervision and Financial Management are described. (LBH)

Protein complex formation and intranuclear dynamics of NAC1 in cancer cells.

PubMed

Nakayama, Naomi; Kato, Hiroaki; Sakashita, Gyosuke; Nariai, Yuko; Nakayama, Kentaro; Kyo, Satoru; Urano, Takeshi

2016-09-15

Nucleus accumbens-associated protein 1 (NAC1) is a cancer-related transcription regulator protein that is also involved in the pluripotency and differentiation of embryonic stem cells. NAC1 is overexpressed in various carcinomas including ovarian, cervical, breast, and pancreatic carcinomas. NAC1 knock-down was previously shown to result in the apoptosis of ovarian cancer cell lines and to rescue their sensitivity to chemotherapy, suggesting that NAC1 may be a potential therapeutic target, but protein complex formation and the dynamics of intranuclear NAC1 in cancer cells remain poorly understood. In this study, analysis of HeLa cell lysates by fast protein liquid chromatography (FPLC) on a sizing column showed that the NAC1 peak corresponded to an apparent molecular mass of 300-500 kDa, which is larger than the estimated molecular mass (58 kDa) of the protein. Furthermore, live cell photobleaching analyses with green fluorescent protein (GFP)-fused NAC1 proteins revealed the intranuclear dynamics of NAC1. Collectively our results demonstrate that NAC1 forms a protein complex to function as a transcriptional regulator in cancer cells. Copyright © 2016 Elsevier Inc. All rights reserved.

78 FR 9743 - NASA Advisory Council; Education and Public Outreach Committee; Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-02-11

... Committee, National Aeronautics and Space Administration, Washington, DC 20456, at [email protected]nasa.gov...://www.nasa.gov/offices/nac/EPO_Meetings.html . SUPPLEMENTARY INFORMATION: The agenda for the meeting... advance by contacting Erika Vick via email at [email protected]nasa.gov or by telephone at (202) 358- 2209 or...

76 FR 31642 - Committee on Equal Opportunities in Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-01

... NATIONAL SCIENCE FOUNDATION Committee on Equal Opportunities in Science and Engineering; Notice of... Engineering (1173). Dates/Time: June 13, 2011, 9 a.m.-4:30 p.m. June 14, 2011, 9 a.m.-2 p.m. Places: June 13... participation in science and engineering. Agenda Monday, June 13, 2011 Opening Statement by the CEOSE Chair...

Overexpression of a Novel Apple NAC Transcription Factor Gene, MdNAC1, Confers the Dwarf Phenotype in Transgenic Apple (Malus domestica)

PubMed Central

Jia, Dongfeng; Gong, Xiaoqing; Li, Mingjun; Li, Chao; Sun, Tingting

2018-01-01

Plant height is an important trait for fruit trees. The dwarf characteristic is commonly associated with highly efficient fruit production, a major objective when breeding for apple (Malus domestica). We studied the function of MdNAC1, a novel NAC transcription factor (TF) gene in apple related to plant dwarfing. Localized primarily to the nucleus, MdNAC1 has transcriptional activity in yeast cells. Overexpression of the gene results in a dwarf phenotype in transgenic apple plants. Their reduction in size is manifested by shorter, thinner stems and roots, and a smaller leaf area. The transgenics also have shorter internodes and fewer cells in the stems. Levels of endogenous abscisic acid (ABA) and brassinosteroid (BR) are lower in the transgenic plants, and expression is decreased for genes involved in the biosynthesis of those phytohormones. All of these findings demonstrate that MdNAC1 has a role in plants dwarfism, probably by regulating ABA and BR production. PMID:29702625

The Federal Science, Technology, Engineering, and Mathematics (STEM) Education Portfolio. A Report from the Federal Inventory of STEM Education Fast-Track Action Committee Committee on STEM Education National Science and Technology Council

ERIC Educational Resources Information Center

Executive Office of the President, 2011

2011-01-01

The National Science and Technology Council (NSTC) Committee on STEM Education (CoSTEM) coordinates Federal programs and activities in support of STEM (science, technology, engineering and mathematics) education pursuant to the requirements of Sec. 101 of the America COMPETES (Creating Opportunities to Meaningfully Promote Excellence in Technology…

O-GlcNAc cycling in the developing, adult and geriatric brain.

PubMed

Lagerlöf, Olof

2018-06-01

Hundreds of proteins in the nervous system are modified by the monosaccharide O-GlcNAc. A single protein is often O-GlcNAcylated on several amino acids and the modification of a single site can play a crucial role for the function of the protein. Despite its complexity, only two enzymes add and remove O-GlcNAc from proteins, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Global and local regulation of these enzymes make it possible for O-GlcNAc to coordinate multiple cellular functions at the same time as regulating specific pathways independently from each other. If O-GlcNAcylation is disrupted, metabolic disorder or intellectual disability may ensue, depending on what neurons are affected. O-GlcNAc's promise as a clinical target for developing drugs against neurodegenerative diseases has been recognized for many years. Recent literature puts O-GlcNAc in the forefront among mechanisms that can help us better understand how neuronal circuits integrate diverse incoming stimuli such as fluctuations in nutrient supply, metabolic hormones, neuronal activity and cellular stress. Here the functions of O-GlcNAc in the nervous system are reviewed.

[A girl with self-harm treated with N-acetylcysteine (NAC)].

PubMed

Rus, C P

Deliberate and recurrent self-harm could be regarded as addictive behaviour that can be treated with medication. In addiction, the dopaminergic mesolimbic reward system is activated. Pain caused by cutting stimulates the reward system through the opioid system. Glutamatergic neurotransmission follows the same pathway and plays a role in addiction as well. In this case-study a 17-year-old girl was successfully treated with N-acetylcysteine (nac) in order to reduce the frequency of self-cutting. In addition, in this case nac reduced the symptoms of attention deficit/hyperactivity disorder and depression. nac modulates the glutamatergic neurotransmission. This article provides possible explanations for the effect of nac in this case.

76 FR 61118 - Advisory Committee for Computer and Information Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-10-03

... Engineering; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as... Computer and Information Science and Engineering (1115). Date and Time: November 1, 2011 from 12 p.m.-5:30... Computer and Information Science and Engineering, National Science Foundation, 4201 Wilson Blvd., Suite...

Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.

PubMed

Toleman, Clifford A; Schumacher, Maria A; Yu, Seok-Ho; Zeng, Wenjie; Cox, Nathan J; Smith, Timothy J; Soderblom, Erik J; Wands, Amberlyn M; Kohler, Jennifer J; Boyce, Michael

2018-06-05

O-GlcNAc is an intracellular posttranslational modification that governs myriad cell biological processes and is dysregulated in human diseases. Despite this broad pathophysiological significance, the biochemical effects of most O-GlcNAcylation events remain uncharacterized. One prevalent hypothesis is that O-GlcNAc moieties may be recognized by "reader" proteins to effect downstream signaling. However, no general O-GlcNAc readers have been identified, leaving a considerable gap in the field. To elucidate O-GlcNAc signaling mechanisms, we devised a biochemical screen for candidate O-GlcNAc reader proteins. We identified several human proteins, including 14-3-3 isoforms, that bind O-GlcNAc directly and selectively. We demonstrate that 14-3-3 proteins bind O-GlcNAc moieties in human cells, and we present the structures of 14-3-3β/α and γ bound to glycopeptides, providing biophysical insights into O-GlcNAc-mediated protein-protein interactions. Because 14-3-3 proteins also bind to phospho-serine and phospho-threonine, they may integrate information from O-GlcNAc and O-phosphate signaling pathways to regulate numerous physiological functions.

Comprehensive analysis and discovery of drought-related NAC transcription factors in common bean.

PubMed

Wu, Jing; Wang, Lanfen; Wang, Shumin

2016-09-07

Common bean (Phaseolus vulgaris L.) is an important warm-season food legume. Drought is the most important environmental stress factor affecting large areas of common bean via plant death or reduced global production. The NAM, ATAF1/2 and CUC2 (NAC) domain protein family are classic transcription factors (TFs) involved in a variety of abiotic stresses, particularly drought stress. However, the NAC TFs in common bean have not been characterized. In the present study, 86 putative NAC TF proteins were identified from the common bean genome database and located on 11 common bean chromosomes. The proteins were phylogenetically clustered into 8 distinct subfamilies. The gene structure and motif composition of common bean NACs were similar in each subfamily. These results suggest that NACs in the same subfamily may possess conserved functions. The expression patterns of common bean NAC genes were also characterized. The majority of NACs exhibited specific temporal and spatial expression patterns. We identified 22 drought-related NAC TFs based on transcriptome data for drought-tolerant and drought-sensitive genotypes. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression patterns of the 20 drought-related NAC genes. Based on the common bean genome sequence, we analyzed the structural characteristics, genome distribution, and expression profiles of NAC gene family members and analyzed drought-responsive NAC genes. Our results provide useful information for the functional characterization of common bean NAC genes and rich resources and opportunities for understanding common bean drought stress tolerance mechanisms.

K-12th Grade Math and Science Education: The View from the Blackboard. Hearing Before the Committee on Science, House of Representatives, 107th Congress, First Session (March 7, 2001).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House.

The hearing reported in this document focuses on mathematics and science education at the K-12 grade levels. The hearing includes the opening statements of Representative Sherwood Boehlert, Chairman, Committee on Science, U.S. House of Representatives and Representative Ralph Hall, Ranking Minority Member, Committee on Science, U.S. House of…

An apple NAC transcription factor negatively regulates cold tolerance via CBF-dependent pathway.

PubMed

An, Jian-Ping; Li, Rui; Qu, Feng-Jia; You, Chun-Xiang; Wang, Xiao-Fei; Hao, Yu-Jin

2018-02-01

Cold stress is an adverse stimulus that affects plant growth and development, and the C-repeat binding factor (CBF) cold-regulatory cascade has been regarded as a master regulator in the plant response to cold stress. Here, we showed that a NAC transcription factor modulated low-temperature tolerance. MdNAC029/MdNAP, an apple NAC gene was isolated and its role in regulating cold tolerance was investigated. MdNAC029 was responsive to low-temperature treatment, and over-expression of MdNAC029 reduced cold tolerance in apple calli and Arabidopsis. Furthermore, EMSA assays and transient expression assays demonstrated that MdNAC029 directly repressed the expression of MdCBF1 and MdCBF4 by binding to their promoters. Taken together, our data suggest that MdNAC029 functions as a negative regulator in regulating plant cold tolerance in a CBF-dependent manner, providing a deeper understanding of NAC transcription-factor-mediated cold tolerance. Copyright © 2017 Elsevier GmbH. All rights reserved.

75 FR 3898 - Federal Advisory Committee; Defense Science Board; Closed Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-01-25

.... national defense posture and homeland security. DATES: The meeting will be held on February 24 and 25, 2010..., and policies as they may affect the U.S. national defense posture and homeland security. In accordance... DEPARTMENT OF DEFENSE Office of the Secretary Federal Advisory Committee; Defense Science Board...

Functional O-GlcNAc modifications: Implications in molecular regulation and pathophysiology

PubMed Central

Wells, Lance

2016-01-01

O-linked β-N-acetylglucosamine (O-GlcNAc) is a regulatory post-translational modification of intracellular proteins. The dynamic and inducible cycling of the modification is governed by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) in response to UDP-GlcNAc levels in the hexosamine biosynthetic pathway (HBP). Due to its reliance on glucose flux and substrate availability, a major focus in the field has been on how O-GlcNAc contributes to metabolic disease. For years this post-translational modification has been known to modify thousands of proteins implicated in various disorders, but direct functional connections have until recently remained elusive. New research is beginning to reveal the specific mechanisms through which O-GlcNAc influences cell dynamics and disease pathology including clear examples of O-GlcNAc modification at a specific site on a given protein altering its biological functions. The following review intends to focus primarily on studies in the last half decade linking O-GlcNAc modification of proteins with chromatin-directed gene regulation, developmental processes, and several metabolically related disorders including Alzheimer’s, heart disease and cancer. These studies illustrate the emerging importance of this post-translational modification in biological processes and multiple pathophysiologies. PMID:24524620

77 FR 24538 - Advisory Committee for Computer and Information Science And Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-04-24

... Engineering; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as... Computer and Information Science and Engineering (1115). Date and Time: May 10, 2012 12 p.m.-5:30 p.m., May... Science and Engineering, National Science Foundation, 4201 Wilson Blvd., Suite 1105, Arlington VA 22230...

First NAC Image Obtained in Mercury Orbit

NASA Image and Video Library

2017-12-08

NASA image acquired: March 29, 2011 This is the first image of Mercury taken from orbit with MESSENGER’s Narrow Angle Camera (NAC). MESSENGER’s camera system, the Mercury Dual Imaging System (MDIS), has two cameras: the Narrow Angle Camera and the Wide Angle Camera (WAC). Comparison of this image with MESSENGER’s first WAC image of the same region shows the substantial difference between the fields of view of the two cameras. At 1.5°, the field of view of the NAC is seven times smaller than the 10.5° field of view of the WAC. This image was taken using MDIS’s pivot. MDIS is mounted on a pivoting platform and is the only instrument in MESSENGER’s payload capable of movement independent of the spacecraft. The other instruments are fixed in place, and most point down the spacecraft’s boresight at all times, relying solely on the guidance and control system for pointing. The 90° range of motion of the pivot gives MDIS a much-needed extra degree of freedom, allowing MDIS to image the planet’s surface at times when spacecraft geometry would normally prevent it from doing so. The pivot also gives MDIS additional imaging opportunities by allowing it to view more of the surface than that at which the boresight-aligned instruments are pointed at any given time. On March 17, 2011 (March 18, 2011, UTC), MESSENGER became the first spacecraft ever to orbit the planet Mercury. The mission is currently in the commissioning phase, during which spacecraft and instrument performance are verified through a series of specially designed checkout activities. In the course of the one-year primary mission, the spacecraft's seven scientific instruments and radio science investigation will unravel the history and evolution of the Solar System's innermost planet. Visit the Why Mercury? section of this website to learn more about the science questions that the MESSENGER mission has set out to answer. Credit: NASA/Johns Hopkins University Applied Physics Laboratory

Revisiting the human polypeptide GalNAc-T1 and T13 paralogs

PubMed Central

Festari, María Florencia; Trajtenberg, Felipe; Berois, Nora; Pantano, Sergio; Revoredo, Leslie; Kong, Yun; Solari-Saquieres, Patricia; Narimatsu, Yoshiki; Freire, Teresa; Bay, Sylvie; Robello, Carlos; Bénard, Jean; Gerken, Thomas A; Clausen, Henrik; Osinaga, Eduardo

2017-01-01

Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isoforms. Here, we present a comprehensive in vitro analysis of the peptide substrate specificity of GalNAc-T13, showing that it essentially overlaps with the ubiquitous expressed GalNAc-T1 isoform found in the same subfamily as T13. We have also identified and partially characterized nine splice variants of GalNAc-T13, which add further complexity to the GalNAc-T family. Two variants with changes in their lectin domains were characterized by in vitro glycosylation assays, and one (Δ39Ex9) was inactive while the second one (Ex10b) had essentially unaltered activity. We used reverse transcription-polymerase chain reaction analysis of human neuroblastoma cell lines, normal brain and a small panel of neuroblastoma tumors to demonstrate that several splice variants (Ex10b, ΔEx9, ΔEx2-7 and ΔEx6/8-39bpEx9) were highly expressed in tumor cell lines compared with normal brain, although the functional implications remain to be unveiled. In summary, the GalNAc-T13 isoform is predicted to function similarly to GalNAc-T1 against peptide substrates in vivo, in contrast to a prior report, but is unique by being selectively expressed in the brain. PMID:27913570

78 FR 38736 - Committee Management; Renewals

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-27

... NATIONAL SCIENCE FOUNDATION Committee Management; Renewals The National Science Foundation (NSF) management officials having responsibility for the advisory committees listed below have determined that.... This determination follows consultation with the Committee Management Secretariat, General Services...

76 FR 37380 - Committee Management Renewals

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-27

... NATIONAL SCIENCE FOUNDATION Committee Management Renewals The National Science Foundation (NSF) management officials having responsibility for the advisory committees listed below have determined that.... This determination follows consultation with the Committee Management Secretariat, General Services...

Section of Cybernetics in Mining of Mining Committee of Polish Academy of Sciences - Pro Memoria

NASA Astrophysics Data System (ADS)

Wojaczek, Antoni; Miśkiewicz, Kazimierz

2017-09-01

Section of Cybernetics in Mining of Mining Committee of Polish Academy of Science (PAN) has been created by PAN Mining Committee in 1969. It was a section in Mining Committee of PAN, whose operation range included widely understood issues of automation, telecommunication and informatics in mining industry. The main operation method of the Section was to organize the periodic conferences dedicated to issues of control systems in mining. The first conference took place in 1971 in Katowice. Together with new (the current one) term of office of Mining Committee of PAN this Section ceased to exist. The paper presents (pro memoria) over 40 year long conference output of this Section that functioned within the scope of operation of Mining Committee of PAN up to 12th January 2016.

Bivalent Carbohydrate Binding Is Required for Biological Activity of Clitocybe nebularis Lectin (CNL), the N,N′-Diacetyllactosediamine (GalNAcβ1–4GlcNAc, LacdiNAc)-specific Lectin from Basidiomycete C. nebularis*

PubMed Central

Pohleven, Jure; Renko, Miha; Magister, Špela; Smith, David F.; Künzler, Markus; Štrukelj, Borut; Turk, Dušan; Kos, Janko; Sabotič, Jerica

2012-01-01

Lectins are carbohydrate-binding proteins that exert their biological activity by binding to specific cell glycoreceptors. We have expressed CNL, a ricin B-like lectin from the basidiomycete Clitocybe nebularis in Escherichia coli. The recombinant lectin, rCNL, agglutinates human blood group A erythrocytes and is specific for the unique glycan N,N′-diacetyllactosediamine (GalNAcβ1–4GlcNAc, LacdiNAc) as demonstrated by glycan microarray analysis. We here describe the crystal structures of rCNL in complex with lactose and LacdiNAc, defining its interactions with the sugars. CNL is a homodimeric lectin, each of whose monomers consist of a single ricin B lectin domain with its β-trefoil fold and one carbohydrate-binding site. To study the mode of CNL action, a nonsugar-binding mutant and nondimerizing monovalent CNL mutants that retain carbohydrate-binding activity were prepared. rCNL and the mutants were examined for their biological activities against Jurkat human leukemic T cells and the hypersensitive nematode Caenorhabditis elegans mutant strain pmk-1. rCNL was toxic against both, although the mutants were inactive. Thus, the bivalent carbohydrate-binding property of homodimeric CNL is essential for its activity, providing one of the rare pieces of evidence that certain activities of lectins are associated with their multivalency. PMID:22298779

O-GlcNAcase Expression is Sensitive to Changes in O-GlcNAc Homeostasis.

PubMed

Zhang, Zhen; Tan, Ee Phie; VandenHull, Nicole J; Peterson, Kenneth R; Slawson, Chad

2014-01-01

O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification involving an attachment of a single β-N-acetylglucosamine moiety to serine or threonine residues in nuclear and cytoplasmic proteins. Cellular O-GlcNAc levels are regulated by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which add and remove the modification, respectively. The levels of O-GlcNAc can rapidly change in response to fluctuations in the extracellular environment; however, O-GlcNAcylation returns to a baseline level quickly after stimulus removal. This process termed O-GlcNAc homeostasis appears to be critical to the regulation of many cellular functions including cell cycle progress, stress response, and gene transcription. Disruptions in O-GlcNAc homeostasis are proposed to lead to the development of diseases, such as cancer, diabetes, and Alzheimer's disease. O-GlcNAc homeostasis is correlated with the expression of OGT and OGA. We reason that alterations in O-GlcNAc levels affect OGA and OGT transcription. We treated several human cell lines with Thiamet-G (TMG, an OGA inhibitor) to increase overall O-GlcNAc levels resulting in decreased OGT protein expression and increased OGA protein expression. OGT transcript levels slightly declined with TMG treatment, but OGA transcript levels were significantly increased. Pretreating cells with protein translation inhibitor cycloheximide did not stabilize OGT or OGA protein expression in the presence of TMG; nor did TMG stabilize OGT and OGA mRNA levels when cells were treated with RNA transcription inhibitor actinomycin D. Finally, we performed RNA Polymerase II chromatin immunoprecipitation at the OGA promoter and found that RNA Pol II occupancy at the transcription start site was lower after prolonged TMG treatment. Together, these data suggest that OGA transcription was sensitive to changes in O-GlcNAc homeostasis and was potentially regulated by O-GlcNAc.

O-GlcNAcase Expression is Sensitive to Changes in O-GlcNAc Homeostasis

PubMed Central

Zhang, Zhen; Tan, Ee Phie; VandenHull, Nicole J.; Peterson, Kenneth R.; Slawson, Chad

2014-01-01

O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification involving an attachment of a single β-N-acetylglucosamine moiety to serine or threonine residues in nuclear and cytoplasmic proteins. Cellular O-GlcNAc levels are regulated by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), which add and remove the modification, respectively. The levels of O-GlcNAc can rapidly change in response to fluctuations in the extracellular environment; however, O-GlcNAcylation returns to a baseline level quickly after stimulus removal. This process termed O-GlcNAc homeostasis appears to be critical to the regulation of many cellular functions including cell cycle progress, stress response, and gene transcription. Disruptions in O-GlcNAc homeostasis are proposed to lead to the development of diseases, such as cancer, diabetes, and Alzheimer’s disease. O-GlcNAc homeostasis is correlated with the expression of OGT and OGA. We reason that alterations in O-GlcNAc levels affect OGA and OGT transcription. We treated several human cell lines with Thiamet-G (TMG, an OGA inhibitor) to increase overall O-GlcNAc levels resulting in decreased OGT protein expression and increased OGA protein expression. OGT transcript levels slightly declined with TMG treatment, but OGA transcript levels were significantly increased. Pretreating cells with protein translation inhibitor cycloheximide did not stabilize OGT or OGA protein expression in the presence of TMG; nor did TMG stabilize OGT and OGA mRNA levels when cells were treated with RNA transcription inhibitor actinomycin D. Finally, we performed RNA Polymerase II chromatin immunoprecipitation at the OGA promoter and found that RNA Pol II occupancy at the transcription start site was lower after prolonged TMG treatment. Together, these data suggest that OGA transcription was sensitive to changes in O-GlcNAc homeostasis and was potentially regulated by O-GlcNAc. PMID:25520704

Catfish (Clarias batrachus) serum lectin recognizes polyvalent Tn [alpha-D-GalpNAc1-Ser/Thr], Talpha [beta-D-Galp-(1-->3)-alpha-D-GalpNAc1-Ser/Thr], and II [beta-D-Galp(1-->4)-beta-D-GlcpNAc1-] mammalian glycotopes.

PubMed

Singha, Biswajit; Adhya, Mausumi; Chatterjee, Bishnu P

2008-09-22

A new calcium dependent GalNAc/Gal specific lectin was isolated from the serum of Indian catfish, Clarias batrachus and designated as C. batrachus lectin (CBL). It is a disulfide-linked homodecameric lectin of 74.65kDa subunits and the oligomeric form is essential for its activity. Binding specificity of CBL was investigated by enzyme-linked lectin-sorbent assay using a series of simple sugars, polysaccharides, and glycoproteins. GalNAc was more potent inhibitor than Gal; and alpha glycosides of both were more inhibitory than their beta counterparts. CBL showed maximum affinity for human tumor-associated Tn-antigens (GalNAcalpha1-Ser/Thr) at the molecular level and was 3.5 times higher than GalNAc. CBL interacted strongly with polyvalent Tn and Talpha (Galbeta1,3GalNAcalpha1-) as well as multivalent-II (Galbeta1,4GlcNAcbeta1-) antigens containing glycoproteins and intensity of inhibition was 10(3)-10(5) times more than monovalent ones. The overall specificity of CBL lies in the order of polyvalent Tn, Talpha and II>monovalent Tn > or = Me-alphaGalNAc>monovalent Talpha> Me-betaGalNAc>Me-alphaGal>monovalent T>GalNAc>monovalent F>monovalent II>Me-betaGal>Gal.

Survey of O-GlcNAc level variations in Xenopus laevis from oogenesis to early development.

PubMed

Dehennaut, Vanessa; Lefebvre, Tony; Leroy, Yves; Vilain, Jean-Pierre; Michalski, Jean-Claude; Bodart, Jean-François

2009-04-01

Little is known about the impact of O-linked-N-acetylglucosaminylation (O-GlcNAc) in gametes production and developmental processes. Here we investigated changes in O-GlcNAc, UDP-GlcNAc and O-GlcNAc transferase (OGT) levels in Xenopus laevis from oogenesis to embryo hatching. We showed that in comparison to stage VI, stages I-V oocytes expressed higher levels of O-GlcNAc correlating changes in OGT expression, but not in UDP-GlcNAc pools. Upon progesterone stimulation, an O-GlcNAc level burst occurred during meiotic resumption long before MPF and Mos-Erk2 pathways activations. Finally, we observed high levels of O-GlcNAc, UDP-GlcNAc and OGT during segmentation that decreased concomitantly at the onset of gastrulation. Nevertheless, no correlation between the glycosylation, the nucleotide-sugar and the glycosyltransferase was observed after neurulation. Our results show that O-GlcNAc is regulated throughout oogenesis and development within a complex pattern and suggest that dysfunctions in the dynamics of this glycosylation could lead to developmental abnormalities.

77 FR 9731 - Voluntary Service National Advisory Committee; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-17

...) National Advisory Committee (NAC) will be held March 14-16, 2012, at the Charleston Marriott, 170 Lockwood Boulevard, Charleston, South Carolina. On March 14, the meeting will begin at 8 a.m. and end at 11:30 a.m. On March 15-16, the meeting will begin at 8:30 a.m. and end at 4:30 p.m. The meeting is open to the...

Genome-Wide Analysis of the NAC Gene Family in Physic Nut (Jatropha curcas L.).

PubMed

Wu, Zhenying; Xu, Xueqin; Xiong, Wangdan; Wu, Pingzhi; Chen, Yaping; Li, Meiru; Wu, Guojiang; Jiang, Huawu

2015-01-01

The NAC proteins (NAM, ATAF1/2 and CUC2) are plant-specific transcriptional regulators that have a conserved NAM domain in the N-terminus. They are involved in various biological processes, including both biotic and abiotic stress responses. In the present study, a total of 100 NAC genes (JcNAC) were identified in physic nut (Jatropha curcas L.). Based on phylogenetic analysis and gene structures, 83 JcNAC genes were classified as members of, or proposed to be diverged from, 39 previously predicted orthologous groups (OGs) of NAC sequences. Physic nut has a single intron-containing NAC gene subfamily that has been lost in many plants. The JcNAC genes are non-randomly distributed across the 11 linkage groups of the physic nut genome, and appear to be preferentially retained duplicates that arose from both ancient and recent duplication events. Digital gene expression analysis indicates that some of the JcNAC genes have tissue-specific expression profiles (e.g. in leaves, roots, stem cortex or seeds), and 29 genes differentially respond to abiotic stresses (drought, salinity, phosphorus deficiency and nitrogen deficiency). Our results will be helpful for further functional analysis of the NAC genes in physic nut.

Activities of the Japan Society of Applied Physics Committee for Diversity Promotion in Science and Technology (abstract)

NASA Astrophysics Data System (ADS)

Nishitani-Gamo, Mikka

2009-04-01

Since 2001, the Japan Society of Applied Physics (JSAP) Committee for Diversity Promotion in Science and Technology has worked to promote gender equality, both within and between academic societies, and in society as a whole. Main activities of the Committee are: (1) organizing symposia and informal meetings during domestic JSAP conferences to stimulate discussion and raise awareness; (2) encouraging young researchers in pursuit of their careers through the newly designed "career-explorer mark;" (3) offering childcare at biannual JSAP conferences; and (4) helping future scientists and engineers prepare to lead the fields of science and technology on a global level with the creation of an educational roadmap. In this presentation, recent activities of the JSAP Committee are introduced and reviewed.

Molecular Evolution and Expansion Analysis of the NAC Transcription Factor in Zea mays

PubMed Central

Fan, Kai; Wang, Ming; Miao, Ying; Ni, Mi; Bibi, Noreen; Yuan, Shuna; Li, Feng; Wang, Xuede

2014-01-01

NAC (NAM, ATAF1, 2 and CUC2) family is a plant-specific transcription factor and it controls various plant developmental processes. In the current study, 124 NAC members were identified in Zea mays and were phylogenetically clustered into 13 distinct subfamilies. The whole genome duplication (WGD), especially an additional WGD event, may lead to expanding ZmNAC members. Different subfamily has different expansion rate, and NAC subfamily preference was found during the expansion in maize. Moreover, the duplication events might occur after the divergence of the lineages of Z. mays and S. italica, and segmental duplication seemed to be the dominant pattern for the gene duplication in maize. Furthermore, the expansion of ZmNAC members may be also related to gain and loss of introns. Besides, the restriction of functional divergence was discovered after most of the gene duplication events. These results could provide novel insights into molecular evolution and expansion analysis of NAC family in maize, and advance the NAC researches in other plants, especially polyploid plants. PMID:25369196

N-acetylcysteine (NAC) in neurological disorders: mechanisms of action and therapeutic opportunities

PubMed Central

Bavarsad Shahripour, Reza; Harrigan, Mark R; Alexandrov, Andrei V

2014-01-01

Background There is an expanding field of research investigating the benefits of medicines with multiple mechanisms of action across neurological disorders. N-acetylcysteine (NAC), widely known as an antidote to acetaminophen overdose, is now emerging as treatment of vascular and nonvascular neurological disorders. NAC as a precursor to the antioxidant glutathione modulates glutamatergic, neurotrophic, and inflammatory pathways. Aim and discussion Most NAC studies up to date have been carried out in animal models of various neurological disorders with only a few studies completed in humans. In psychiatry, NAC has been tested in over 20 clinical trials as an adjunctive treatment; however, this topic is beyond the scope of this review. Herein, we discuss NAC molecular, intracellular, and systemic effects, focusing on its potential applications in neurodegenerative diseases including spinocerebellar ataxia, Parkinson's disease, tardive dyskinesia, myoclonus epilepsy of the Unverricht–Lundbor type as well as multiple sclerosis, amyotrophic lateral sclerosis, and Alzheimer's disease. Conclusion Finally, we review the potential applications of NAC to facilitate recovery after traumatic brain injury, cerebral ischemia, and in treatment of cerebrovascular vasospasm after subarachnoid hemorrhage. PMID:24683506

O-GlcNAc modification of PPAR{gamma} reduces its transcriptional activity

DOE Office of Scientific and Technical Information (OSTI.GOV)

Ji, Suena; Park, Sang Yoon; Roth, Juergen

2012-01-27

Highlights: Black-Right-Pointing-Pointer We found that PPAR{gamma} is modified by O-GlcNAc in 3T3-L1 adipocytes. Black-Right-Pointing-Pointer The Thr54 of PPAR{gamma}1 is the major O-GlcNAc site. Black-Right-Pointing-Pointer Transcriptional activity of PPAR{gamma}1 was decreased on treatment with the OGA inhibitor. -- Abstract: The peroxisome proliferator-activated receptor {gamma} (PPAR{gamma}), a member of the nuclear receptor superfamily, is a key regulator of adipogenesis and is important for the homeostasis of the adipose tissue. The {beta}-O-linked N-acetylglucosamine (O-GlcNAc) modification, a posttranslational modification on various nuclear and cytoplasmic proteins, is involved in the regulation of protein function. Here, we report that PPAR{gamma} is modified by O-GlcNAc in 3T3-L1more » adipocytes. Mass spectrometric analysis and mutant studies revealed that the threonine 54 of the N-terminal AF-1 domain of PPAR{gamma} is the major O-GlcNAc site. Transcriptional activity of wild type PPAR{gamma} was decreased 30% by treatment with the specific O-GlcNAcase (OGA) inhibitor, but the T54A mutant of PPAR{gamma} did not respond to inhibitor treatment. In 3T3-L1 cells, an increase in O-GlcNAc modification by OGA inhibitor reduced PPAR{gamma} transcriptional activity and terminal adipocyte differentiation. Our results suggest that the O-GlcNAc state of PPAR{gamma} influences its transcriptional activity and is involved in adipocyte differentiation.« less

Genome-Wide Analysis of the NAC Gene Family in Physic Nut (Jatropha curcas L.)

PubMed Central

Wu, Zhenying; Xu, Xueqin; Xiong, Wangdan; Wu, Pingzhi; Chen, Yaping; Li, Meiru; Wu, Guojiang; Jiang, Huawu

2015-01-01

The NAC proteins (NAM, ATAF1/2 and CUC2) are plant-specific transcriptional regulators that have a conserved NAM domain in the N-terminus. They are involved in various biological processes, including both biotic and abiotic stress responses. In the present study, a total of 100 NAC genes (JcNAC) were identified in physic nut (Jatropha curcas L.). Based on phylogenetic analysis and gene structures, 83 JcNAC genes were classified as members of, or proposed to be diverged from, 39 previously predicted orthologous groups (OGs) of NAC sequences. Physic nut has a single intron-containing NAC gene subfamily that has been lost in many plants. The JcNAC genes are non-randomly distributed across the 11 linkage groups of the physic nut genome, and appear to be preferentially retained duplicates that arose from both ancient and recent duplication events. Digital gene expression analysis indicates that some of the JcNAC genes have tissue-specific expression profiles (e.g. in leaves, roots, stem cortex or seeds), and 29 genes differentially respond to abiotic stresses (drought, salinity, phosphorus deficiency and nitrogen deficiency). Our results will be helpful for further functional analysis of the NAC genes in physic nut. PMID:26125188

Hyaluronan synthase assembles hyaluronan on a [GlcNAc(β1,4)]n-GlcNAc(α1→)UDP primer and hyaluronan retains this residual chitin oligomer as a cap at the nonreducing end

PubMed Central

Baggenstoss, Bruce A; Washburn, Jennifer L

2017-01-01

Abstract Class I hyaluronan synthases (HAS) assemble [GlcNAc(β1,4)GlcUA(β1,3)]n-UDP at the reducing end and also make chitin. Streptococcus equisimilis HAS (SeHAS) also synthesizes chitin-UDP oligosaccharides, (GlcNAc-β1,4)n-GlcNAc(α1→)UDP (Weigel et al. 2015). Here we determined if HAS uses chitin-UDPs as primers to initiate HA synthesis, leaving the non-HA primer at the nonreducing (NR) end. HA made by SeHAS membranes was purified, digested with streptomyces lyase, and hydrophobic oligomers were enriched by solid phase extraction and analyzed by MALDI-TOF MS. Jack bean hexosaminidase (JBH) and MS/MS were used to analyze 19 m/z species of possible GnHn ions with clustered GlcNAc (G) residues attached to disaccharide units (H): (GlcNAcβ1,4)2–5[GlcUA(β1,3)GlcNAc]2–6. JBH digestion sequentially removed GlcNAc from the NR-end of GnHn oligomers, producing successively smaller GnH2–3 series members. Since lyase releases dehydro-oligos (dHn; M−18), only the unique NR-end oligo lacks dehydro-GlcUA. Hn oligomers were undetectable in lyase digests, whereas JBH treatment created new H2–6m/z peaks (i.e. HA tetra- through dodeca-oligomers). MS/MS of larger GnHn species produced chitin (2–5 GlcNAcs), HA oligomers and multiple smaller series members with fewer GlcNAcs. All NR-ends (97%) started with GlcNAc, as a chitin trimer (three GlcNAcs), indicating that GlcNAc(β1,4)2GlcNAc(α1→)-UDP may be optimal for initiation of HA synthesis. Also, HA made by live S. pyogenes cells had G4Hn chitin-oligo NR-ends. We conclude that chitin-UDP functions in vitro and in live cells as a primer to initiate synthesis of all HA chains and these primers remain at the NR-ends of HA chains as residual chitin caps [(GlcNAc-β1,4)3–4]. PMID:28138013

Hyaluronan synthase assembles hyaluronan on a [GlcNAc(β1,4)]n-GlcNAc(α1→)UDP primer and hyaluronan retains this residual chitin oligomer as a cap at the nonreducing end.

PubMed

Weigel, Paul H; Baggenstoss, Bruce A; Washburn, Jennifer L

2017-06-01

Class I hyaluronan synthases (HAS) assemble [GlcNAc(β1,4)GlcUA(β1,3)]n-UDP at the reducing end and also make chitin. Streptococcus equisimilis HAS (SeHAS) also synthesizes chitin-UDP oligosaccharides, (GlcNAc-β1,4)n-GlcNAc(α1→)UDP (Weigel et al. 2015). Here we determined if HAS uses chitin-UDPs as primers to initiate HA synthesis, leaving the non-HA primer at the nonreducing (NR) end. HA made by SeHAS membranes was purified, digested with streptomyces lyase, and hydrophobic oligomers were enriched by solid phase extraction and analyzed by MALDI-TOF MS. Jack bean hexosaminidase (JBH) and MS/MS were used to analyze 19 m/z species of possible GnHn ions with clustered GlcNAc (G) residues attached to disaccharide units (H): (GlcNAcβ1,4)2-5[GlcUA(β1,3)GlcNAc]2-6. JBH digestion sequentially removed GlcNAc from the NR-end of GnHn oligomers, producing successively smaller GnH2-3 series members. Since lyase releases dehydro-oligos (dHn; M-18), only the unique NR-end oligo lacks dehydro-GlcUA. Hn oligomers were undetectable in lyase digests, whereas JBH treatment created new H2-6m/z peaks (i.e. HA tetra- through dodeca-oligomers). MS/MS of larger GnHn species produced chitin (2-5 GlcNAcs), HA oligomers and multiple smaller series members with fewer GlcNAcs. All NR-ends (97%) started with GlcNAc, as a chitin trimer (three GlcNAcs), indicating that GlcNAc(β1,4)2GlcNAc(α1→)-UDP may be optimal for initiation of HA synthesis. Also, HA made by live S. pyogenes cells had G4Hn chitin-oligo NR-ends. We conclude that chitin-UDP functions in vitro and in live cells as a primer to initiate synthesis of all HA chains and these primers remain at the NR-ends of HA chains as residual chitin caps [(GlcNAc-β1,4)3-4]. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

77 FR 66873 - Advisory Committee for Computer and Information Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-11-07

... Engineering; Notice of Meeting In accordance with Federal Advisory Committee Act (Pub. L. 92-463, as amended... and Information Science and Engineering (1115). Date/Time: November 28, 2012: 12:00 p.m. to 5:30 p.m... Information Science and Engineering (CISE) community. To provide advice to the Assistant Director for CISE on...

Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening.

PubMed

Shan, Wei; Kuang, Jian-fei; Chen, Lei; Xie, Hui; Peng, Huan-huan; Xiao, Yun-yi; Li, Xue-ping; Chen, Wei-xin; He, Quan-guang; Chen, Jian-ye; Lu, Wang-jin

2012-09-01

The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, and stress responses. However, the precise role of NAC TFs in relation to fruit ripening is poorly understood. In this study, six NAC genes, designated MaNAC1-MaNAC6, were isolated and characterized from banana fruit. Subcellular localization showed that MaNAC1-MaNAC5 proteins localized preferentially to the nucleus, while MaNAC6 was distributed throughout the entire cell. A transactivation assay in yeast demonstrated that MaNAC4 and MaNAC6, as well as their C-terminal regions, possessed trans-activation activity. Gene expression profiles in fruit with four different ripening characteristics, including natural, ethylene-induced, 1-methylcyclopropene (1-MCP)-delayed, and a combination of 1-MCP with ethylene treatment, revealed that the MaNAC genes were differentially expressed in peel and pulp during post-harvest ripening. MaNAC1 and MaNAC2 were apparently upregulated by ethylene in peel and pulp, consistent with the increase in ethylene production. In contrast, MaNAC3 in peel and pulp and MaNAC5 in peel were constitutively expressed, and transcripts of MaNAC4 in peel and pulp and MaNAC6 in peel decreased, while MaNAC5 or MaNAC6 in pulp increased slightly during fruit ripening. Furthermore, the MaNAC2 promoter was activated after ethylene application, further enhancing the involvement of MaNAC2 in fruit ripening. More importantly, yeast two-hybrid and bimolecular fluorescence complementation analyses confirmed that MaNAC1/2 physically interacted with a downstream component of ethylene signalling, ethylene insensitive 3 (EIN3)-like protein, termed MaEIL5, which was downregulated during ripening. Taken together, these results suggest that MaNACs such as MaNAC1/MaNAC2, may be involved in banana fruit ripening via interaction with ethylene signalling components.

Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening

PubMed Central

Shan, Wei; Kuang, Jian-fei; Chen, Jian-ye; Lu, Wang-jin

2012-01-01

The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, and stress responses. However, the precise role of NAC TFs in relation to fruit ripening is poorly understood. In this study, six NAC genes, designated MaNAC1–MaNAC6, were isolated and characterized from banana fruit. Subcellular localization showed that MaNAC1–MaNAC5 proteins localized preferentially to the nucleus, while MaNAC6 was distributed throughout the entire cell. A transactivation assay in yeast demonstrated that MaNAC4 and MaNAC6, as well as their C-terminal regions, possessed trans-activation activity. Gene expression profiles in fruit with four different ripening characteristics, including natural, ethylene-induced, 1-methylcyclopropene (1-MCP)-delayed, and a combination of 1-MCP with ethylene treatment, revealed that the MaNAC genes were differentially expressed in peel and pulp during post-harvest ripening. MaNAC1 and MaNAC2 were apparently upregulated by ethylene in peel and pulp, consistent with the increase in ethylene production. In contrast, MaNAC3 in peel and pulp and MaNAC5 in peel were constitutively expressed, and transcripts of MaNAC4 in peel and pulp and MaNAC6 in peel decreased, while MaNAC5 or MaNAC6 in pulp increased slightly during fruit ripening. Furthermore, the MaNAC2 promoter was activated after ethylene application, further enhancing the involvement of MaNAC2 in fruit ripening. More importantly, yeast two-hybrid and bimolecular fluorescence complementation analyses confirmed that MaNAC1/2 physically interacted with a downstream component of ethylene signalling, ethylene insensitive 3 (EIN3)-like protein, termed MaEIL5, which was downregulated during ripening. Taken together, these results suggest that MaNACs such as MaNAC1/MaNAC2, may be involved in banana fruit ripening via interaction with ethylene signalling components. PMID:22888129

The active site of O-GlcNAc transferase imposes constraints on substrate sequence

PubMed Central

Rafie, Karim; Blair, David E.; Borodkin, Vladimir S.; Albarbarawi, Osama; van Aalten, Daan M. F.

2016-01-01

O-GlcNAc transferase (OGT) glycosylates a diverse range of intracellular proteins with O-linked N-acetylglucosamine (O-GlcNAc), an essential and dynamic post-translational modification in metazoa. Although this enzyme modifies hundreds of proteins with O-GlcNAc, it is not understood how OGT achieves substrate specificity. In this study, we describe the application of a high-throughput OGT assay on a library of peptides. The sites of O-GlcNAc modification were mapped by ETD-mass spectrometry, and found to correlate with previously detected O-GlcNAc sites. Crystal structures of four acceptor peptides in complex with human OGT suggest that a combination of size and conformational restriction defines sequence specificity in the −3 to +2 subsites. This work reveals that while the N-terminal TPR repeats of hOGT may play a role in substrate recognition, the sequence restriction imposed by the peptide-binding site makes a significant contribution to O-GlcNAc site specificity. PMID:26237509

Electrophoretic mobility shift assay reveals a novel recognition sequence for Setaria italica NAC protein.

PubMed

Puranik, Swati; Kumar, Karunesh; Srivastava, Prem S; Prasad, Manoj

2011-10-01

The NAC (NAM/ATAF1,2/CUC2) proteins are among the largest family of plant transcription factors. Its members have been associated with diverse plant processes and intricately regulate the expression of several genes. Inspite of this immense progress, knowledge of their DNA-binding properties are still limited. In our recent publication,1 we reported isolation of a membrane-associated NAC domain protein from Setaria italica (SiNAC). Transactivation analysis revealed that it was a functionally active transcription factor as it could stimulate expression of reporter genes in vivo. Truncations of the transmembrane region of the protein lead to its nuclear localization. Here we describe expression and purification of SiNAC DNA-binding domain. We further report identification of a novel DNA-binding site, [C/G][A/T][T/A][G/C]TC[C/G][A/T][C/G][G/C] for SiNAC by electrophoretic mobility shift assay. The SiNAC-GST protein could bind to the NAC recognition sequence in vitro as well as to sequences where some bases had been reshuffled. The results presented here contribute to our understanding of the DNA-binding specificity of SiNAC protein.

Electrophoretic mobility shift assay reveals a novel recognition sequence for Setaria italica NAC protein

PubMed Central

Puranik, Swati; Kumar, Karunesh; Srivastava, Prem S

2011-01-01

The NAC (NAM/ATAF1,2/CUC2) proteins are among the largest family of plant transcription factors. Its members have been associated with diverse plant processes and intricately regulate the expression of several genes. Inspite of this immense progress, knowledge of their DNA-binding properties are still limited. In our recent publication,1 we reported isolation of a membrane-associated NAC domain protein from Setaria italica (SiNAC). Transactivation analysis revealed that it was a functionally active transcription factor as it could stimulate expression of reporter genes in vivo. Truncation of the transmembrane region of the protein lead to its nuclear localization. Here we describe expression and purification of SiNAC DNA-binding domain. We further report identification of a novel DNA-binding site, [C/G][A/T] [T/A][G/C]TC[C/G][A/T][C/G][G/C] for SiNAC by electrophoretic mobility shift assay. The SiNAC-GST protein could bind to the NAC recognition sequence in vitro as well as to sequences where some bases had been reshuffled. The results presented here contribute to our understanding of the DNA-binding specificity of SiNAC protein. PMID:21918373

76 FR 4645 - Fusion Energy Sciences Advisory Committee; Notice of Open Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-26

..., 9 a.m. to 5 p.m.; Tuesday, March 8, 2011, 8:30 a.m. to 12 p.m. ADDRESSES: Doubletree Bethesda Hotel... year (FY) 2012 budget submission to Congress and to conduct other committee business. Tentative Agenda Items: Office of Science FY 2012 Congressional Budget Request FES Program FY 2012 Congressional Budget...

O-GlcNAc regulates NEDD4-1 stability via caspase-mediated pathway.

PubMed

Jiang, Kuan; Bai, Bingyang; Ta, Yajie; Zhang, Tingling; Xiao, Zikang; Wang, Peng George; Zhang, Lianwen

2016-03-18

O-GlcNAc modification of cytosolic and nuclear proteins regulates essential cellular processes such as stress responses, transcription, translation, and protein degradation. Emerging evidence indicates O-GlcNAcylation has a dynamic interplay with ubiquitination in cellular regulation. Here, we report that O-GlcNAc indirectly targets a vital E3 ubiquitin ligase enzyme of NEDD4-1. The protein level of NEDD4-1 is accordingly decreased following an increase of overall O-GlcNAc level upon PUGNAc or glucosamine stimulation. O-GlcNAc transferase (OGT) knockdown, overexpression and mutation results confirm that the stability of NEDD4-1 is negatively regulated by cellular O-GlcNAc. Moreover, the NEDD4-1 degradation induced by PUGNAc or GlcN is significantly inhibited by the caspase inhibitor. Our study reveals a regulation mechanism of NEDD4-1 stability by O-GlcNAcylation. Copyright © 2016 Elsevier Inc. All rights reserved.

[Cloning, subcellular localization, and heterologous expression of ApNAC1 gene from Andrographis paniculata].

PubMed

Wang, Jian; Qi, Meng-Die; Guo, Juan; Shen, Ye; Lin, Hui-Xin; Huang, Lu-Qi

2017-03-01

Andrographis paniculata is widely used as medicinal herb in China for a long time and andrographolide is its main medicinal constituent. To investigate the underlying andrographolide biosynthesis mechanisms, RNA-seq for A. paniculata leaves with MeJA treatment was performed. In A. paniculata transcriptomic data, the expression pattern of one member of NAC transcription factor family (ApNAC1) matched with andrographolide accumulation. The coding sequence of ApNAC1 was cloned by RT-PCR, and GenBank accession number was KY196416. The analysis of bioinformatics showed that the gene encodes a peptide of 323 amino acids, with a predicted relative molecular weight of 35.9 kDa and isoelectric point of 6.14. To confirm the subcellular localization, ApNAC1-GFP was transiently expressed in A. paniculata protoplast. The results indicated that ApNAC1 is a nucleus-localized protein. The analysis of real-time quantitative PCR revealed that ApNAC1 gene predominantly expresses in leaves. Compared with control sample, its expression abundance sharply increased with methyl jasmonate treatment. Based on its expression pattern, ApNAC1 gene might involve in andrographolide biosynthesis. ApNAC1 was heterologously expressed in Escherichia coli and recombinant protein was purified by Ni-NTA agarose. Further study will help us to understand the function of ApNAC1 in andrographolide biosynthesis. Copyright© by the Chinese Pharmaceutical Association.

Public/Private Sector Interactions: The Implications for Networking. A Discussion Report Prepared by the Network Advisory Committee.

ERIC Educational Resources Information Center

Network Planning Paper, 1983

1983-01-01

At a 2-day meeting in October 1982, the Library of Congress Network Advisory Committee (NAC) members discussed the complex issues involved in public and private sector interactions and their relationship to networking activities. The report, "Public Sector/Private Sector Interaction in Providing Information Services," prepared by the…

Riding the Electronic Wave--Document Delivery: Proceedings of the Library of Congress Network Advisory Committee Meeting (Washington, D.C., November 29-December 1, 1989). Network Planning Paper Number 20.

ERIC Educational Resources Information Center

Library of Congress, Washington, DC. Network Development and MARC Standards Office.

Papers delivered at the 1989 program session of the Library of Congress Network Advisory Committee (NAC) focused on ways in which newer technologies and document delivery networks are changing current practices in document delivery and information services. Charles P. Bourne, chair of the program committee, presented an overview of document…

Hsp70-GlcNAc-binding activity is released by stress, proteasome inhibition, and protein misfolding

DOE Office of Scientific and Technical Information (OSTI.GOV)

Guinez, Celine; Mir, Anne-Marie; Leroy, Yves

2007-09-21

Numerous recent works strengthen the idea that the nuclear and cytosolic-specific O-GlcNAc glycosylation protects cells against injuries. We have first investigated O-GlcNAc level and Hsp70-GlcNAc-binding activity (HGBA) behaviour after exposure of HeLa and HepG{sub 2} cells to a wide variety of stresses. O-GlcNAc and HGBA responses were different according to the stress and according to the cell. HGBA was released for almost all stresses, while O-GlcNAc level was modified either upwards or downwards, depending to the stress. Against all expectations, we demonstrated that energy charge did not significantly vary with stress whereas UDP-GlcNAc pools were more dramatically affected even ifmore » differences in UDP-GlcNAc contents were not correlated with O-GlcNAc variations suggesting that O-GlcNAc transferase is itself finely regulated during cell injury. Finally, HGBA could be triggered by proteasome inhibition and by L-azetidine-2-carboxylic acid (a proline analogue) incorporation demonstrating that protein misfolding is one of the key-activator of this Hsp70 property.« less

75 FR 19428 - Advisory Committee for Computer and Information Science and Engineering; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-14

... Engineering; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as... Computer and Information Science and Engineering--(1115). Date and Time: May 7, 2010, 8:30 a.m.-5 p.m... Information Science and Engineering at 703/292-8900 prior to the meeting so that a visitor's badge may be...

Suicidal inactivation of methemoglobin by generation of thiyl radical: insight into NAC mediated protection in RBC.

PubMed

Balaji, S N; Trivedi, V

2013-07-01

N-acetyl-L-cysteine (NAC) improves antioxidant potentials of RBCs to provide protection against oxidative stress induced hemolysis. The antioxidant mechanism of NAC to reduce oxidative stress in RBC, studied through inactivation of pro-oxidant MetHb. NAC causes irreversible inactivation of the MetHb in an H2O2 dependent manner, and the inactivation follows the pseudo- first- order kinetics. The kinetic constants are ki = 8.5μM, kinact = 0.706 min(-1) and t1/2 = 0.9 min. Spectroscopic studies indicate that MetHb accepts NAC as a substrate and oxidizes through a single electron transfer mechanism to the NACox. The single e- oxidation product of NAC has been identified as the 5, 5'- dimethyl-1- pyrroline N- oxide (DMPO) adduct of the sulfur centered radical (a(N) = 15.2 G and a(H)=16.78 G). Binding studies indicate that NACox interacts at the heme moiety and NAC oxidation through MetHb is essential for NAC binding. Heme-NAC adduct dissociated from MetHb and identified (m/z 1011.19) as 2:1 ratio of NAC/heme in the adduct. TEMPO and PBN treatment reduces NAC binding to MetHb and protects against inactivation confirms the role of thiyl radical in the inactivation process. Furthermore, scavenging thiyl radicals by TEMPO abolish the protective effect of NAC in hemolysis. Current work highlights antioxidant mechanism of NAC through NAC thiyl radical generation, and MetHb inactivation to exhibit protection in RBC against oxidative stress induced hemolysis.

O-GlcNAc transferase regulates transcriptional activity of human Oct4.

PubMed

Constable, Sandii; Lim, Jae-Min; Vaidyanathan, Krithika; Wells, Lance

2017-10-01

O-linked β-N-acetylglucosamine (O-GlcNAc) is a single sugar modification found on many different classes of nuclear and cytoplasmic proteins. Addition of this modification, by the enzyme O-linked N-acetylglucosamine transferase (OGT), is dynamic and inducible. One major class of proteins modified by O-GlcNAc is transcription factors. O-GlcNAc regulates transcription factor properties through a variety of different mechanisms including localization, stability and transcriptional activation. Maintenance of embryonic stem (ES) cell pluripotency requires tight regulation of several key transcription factors, many of which are modified by O-GlcNAc. Octamer-binding protein 4 (Oct4) is one of the key transcription factors required for pluripotency of ES cells and more recently, the generation of induced pluripotent stem (iPS) cells. The action of Oct4 is modulated by the addition of several post-translational modifications, including O-GlcNAc. Previous studies in mice found a single site of O-GlcNAc addition responsible for transcriptional regulation. This study was designed to determine if this mechanism is conserved in humans. We mapped 10 novel sites of O-GlcNAc attachment on human Oct4, and confirmed a role for OGT in transcriptional activation of Oct4 at a site distinct from that found in mouse that allows distinction between different Oct4 target promoters. Additionally, we uncovered a potential new role for OGT that does not include its catalytic function. These results confirm that human Oct4 activity is being regulated by OGT by a mechanism that is distinct from mouse Oct4. © The Author 2017. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Function of Hevea brasiliensis NAC1 in dehydration-induced laticifer differentiation and latex biosynthesis.

PubMed

Cao, Yuxin; Zhai, Jinling; Wang, Qichao; Yuan, Hongmei; Huang, Xi

2017-01-01

HbNAC1 is a transcription factor in rubber plants whose expression is induced by dehydration, leading to latex biosynthesis. Laticifer is a special tissue in Hevea brasiliensis where natural rubber is biosynthesized and accumulated. In young stems of epicormic shoots, the differentiation of secondary laticifers can be induced by wounding, which can be prevented when the wounding site is wrapped. Using this system, differentially expressed genes were screened by suppression subtractive hybridization (SSH) and macroarray analyses. This led to the identification of several dehydration-related genes that could be involved in laticifer differentiation and/or latex biosynthesis, including a NAC transcription factor (termed as HbNAC1). Tissue sections confirmed that local tissue dehydration was a key signal for laticifer differentiation. HbNAC1 was localized at the nucleus and showed strong transcriptional activity in yeast, suggesting that HbNAC1 is a transcription factor. Furthermore, HbNAC1 was found to bind to the cis-element CACG in the promoter region of the gene encoding the small rubber particle protein (SRPP). Transgenic experiments also confirmed that HbNAC1 interacted with the SRPP promoter when co-expressed, and enhanced expression of the reporter gene β-glucuronidase occurred in planta. In addition, overexpression of HbNAC1 in tobacco plants conferred drought tolerance. Together, the data suggest that HbNAC1 might be involved in dehydration-induced laticifer differentiation and latex biosynthesis.

Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners

PubMed Central

Yu, Seok-Ho; Boyce, Michael; Wands, Amberlyn M.; Bond, Michelle R.; Bertozzi, Carolyn R.; Kohler, Jennifer J.

2012-01-01

O-linked β-N-acetylglucosamine (O-GlcNAc) is a reversible posttranslational modification found on hundreds of nuclear and cytoplasmic proteins in higher eukaryotes. Despite its ubiquity and essentiality in mammals, functional roles for the O-GlcNAc modification remain poorly defined. Here we develop a combined genetic and chemical approach that enables introduction of the diazirine photocrosslinker onto the O-GlcNAc modification in cells. We engineered mammalian cells to produce diazirine-modified O-GlcNAc by expressing a mutant form of UDP-GlcNAc pyrophosphorylase and subsequently culturing these cells with a cell-permeable, diazirine-modified form of GlcNAc-1-phosphate. Irradiation of cells with UV light activated the crosslinker, resulting in formation of covalent bonds between O-GlcNAc-modified proteins and neighboring molecules, which could be identified by mass spectrometry. We used this method to identify interaction partners for the O-GlcNAc-modified FG-repeat nucleoporins. We observed crosslinking between FG-repeat nucleoporins and nuclear transport factors, suggesting that O-GlcNAc residues are intimately associated with essential recognition events in nuclear transport. Further, we propose that the method reported here could find widespread use in investigating the functional consequences of O-GlcNAcylation. PMID:22411826

The Role of Stress-Induced O-GlcNAc Protein Modification in the Regulation of Membrane Transport

PubMed Central

Fisi, Viktória; Miseta, Attila

2017-01-01

O-linked N-acetylglucosamine (O-GlcNAc) is a posttranslational modification that is increasingly recognized as a signal transduction mechanism. Unlike other glycans, O-GlcNAc is a highly dynamic and reversible process that involves the addition and removal of a single N-acetylglucosamine molecule to Ser/Thr residues of proteins. UDP-GlcNAc—the direct substrate for O-GlcNAc modification—is controlled by the rate of cellular metabolism, and thus O-GlcNAc is dependent on substrate availability. Serving as a feedback mechanism, O-GlcNAc influences the regulation of insulin signaling and glucose transport. Besides nutrient sensing, O-GlcNAc was also implicated in the regulation of various physiological and pathophysiological processes. Due to improvements of mass spectrometry techniques, more than one thousand proteins were detected to carry the O-GlcNAc moiety; many of them are known to participate in the regulation of metabolites, ions, or protein transport across biological membranes. Recent studies also indicated that O-GlcNAc is involved in stress adaptation; overwhelming evidences suggest that O-GlcNAc levels increase upon stress. O-GlcNAc elevation is generally considered to be beneficial during stress, although the exact nature of its protective effect is not understood. In this review, we summarize the current data regarding the oxidative stress-related changes of O-GlcNAc levels and discuss the implications related to membrane trafficking. PMID:29456783

Cloning and expression of a novel UDP-GlcNAc:alpha-D-mannoside beta1,2-N-acetylglucosaminyltransferase homologous to UDP-GlcNAc:alpha-3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I.

PubMed Central

Zhang, Wenli; Betel, Doron; Schachter, Harry

2002-01-01

A TBLASTN search with human UDP-GlcNAc:alpha-3-d-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101) as a probe identified human and mouse Unigenes encoding a protein similar to human GnT I (34% identity over 340 amino acids). The recombinant protein converted Man(alpha1-6)[Man(alpha1-3)]Man(beta1-)O-octyl to Man(alpha1-6)[GlcNAc(beta1-2)Man(alpha1-3)]Man(beta1-)O-octyl, the reaction catalysed by GnT I. The enzyme also added GlcNAc to Man(alpha1-6)[GlcNAc(beta1-2)Man(alpha1-3)]Man(beta1-)O-octyl (the substrate for beta-1,2-N-acetylglucosaminyltransferase II), Man(alpha1-)O-benzyl [with K(m) values of approximately 0.3 and >30 mM for UDP-GlcNAc and Man(alpha1-)O-benzyl respectively] and the glycopeptide CYA[Man(alpha1-)O-T]AV (K(m) approximately 12 mM). The product formed with Man(alpha1-)O-benzyl was identified as GlcNAc(beta1-2)Man(alpha1-)O-benzyl by proton NMR spectroscopy. The enzyme was named UDP-GlcNAc:alpha-d-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2 (GnT I.2). The human gene mapped to chromosome 1. Northern-blot analysis showed a 3.3 kb message with a wide tissue distribution. The cDNA has a 1980 bp open reading frame encoding a 660 amino acid protein with a type-2 domain structure typical of glycosyltransferases. Man(beta1-)O-octyl, Man(beta1-)O-p-nitrophenyl and GlcNAc(beta1-2)Man(alpha1-6)[GlcNAc(beta1-2)Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc(beta1-)O-Asn were not acceptors, indicating that GnT I.2 is specific for alpha-linked terminal Man and does not have N-acetylglucosaminyltransferase III, IV, V, VII or VIII activities. CYA[Man(alpha1-)O-T]AV was between three and seven times more effective as an acceptor than the other substrates, suggesting that GnT I.2 may be responsible for the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins. PMID:11742540

GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L).

PubMed

Zhao, Fengli; Ma, Jianhui; Li, Libei; Fan, Shuli; Guo, Yaning; Song, Meizhen; Wei, Hengling; Pang, Chaoyou; Yu, Shuxun

2016-01-15

NAC (NAM, ATAF, and CUC) is one of the largest transcription factor families in plants, and its members play various roles in plant growth, development, and the response to biotic and abiotic stresses. Currently, 77 NAC genes have been reported in cotton (Gossypium hirsutum L.). And GhNAC12 showed up-regulation during leaf senescence, but its role in this process is poorly understood. In the present study, a preliminary function analysis of GhNAC12 was performed during leaf senescence. qRT-PCR analysis indicated that GhNAC12 expression increased during the early-aging process and the aging of cotyledons. Additionally, we observed that overexpression of GhNAC12 in Arabidopsis led to early senescence (early aging). Our findings suggest that GhNAC12 is a candidate gene for early aging in upland cotton cultivars. Neutrality tests suggested that there was no selection pressure imposed on GhNAC12 during the domestication of upland cotton. Copyright © 2015 Elsevier B.V. All rights reserved.

Analysis of Sulfate Patterns in Glycosaminoglycan Oligosaccharides by MSn Coupled to Infrared Ion Spectroscopy: the Case of GalNAc4S and GalNAc6S

NASA Astrophysics Data System (ADS)

Renois-Predelus, G.; Schindler, B.; Compagnon, I.

2018-04-01

We report distinctive spectroscopic fingerprints of the monosaccharide standards GalNAc4S and GalNAc6S by coupling mass spectrometry and ion spectroscopy in the 3-μm range. The disaccharide standards CSA and CSC are used to demonstrate the applicability of a novel approach for the analysis of sulfate position in GalNAc-containing glycosaminoglycans. This approach was then used for the analysis of a sample containing CSA and CSC disaccharides. Finally, we discuss the generalization of the coupling of mass spectrometry with ion spectroscopy for the structural analysis of glycosaminoglycans on a tetrasaccharide from dermatan sulfate source. [Figure not available: see fulltext.

N-acetyl cysteine (NAC)-mediated detoxification and functionalization of poly(methyl methacrylate) bone cement.

PubMed

Tsukimura, Naoki; Yamada, Masahiro; Aita, Hideki; Hori, Norio; Yoshino, Fumihiko; Chang-Il Lee, Masaichi; Kimoto, Katsuhiko; Jewett, Anahid; Ogawa, Takahiro

2009-07-01

Currently used poly(methyl methacrylate) (PMMA)-based bone cement lacks osteoconductivity and induces osteolysis and implant loosening due to its cellular and tissue-toxicity. A high percentage of revision surgery following the use of bone cement has become a significant universal problem. This study determined whether incorporation of the amino acid derivative N-acetyl cysteine (NAC) in bone cement reduces its cytotoxicity and adds osteoconductivity to the material. Biocompatibility and bioactivity of PMMA-based bone cement with or without 25mm NAC incorporation was examined using rat bone marrow-derived osteoblastic cells. Osteoconductive potential of NAC-incorporated bone cement was determined by microCT bone morphometry and implant biomechanical test in the rat model. Generation of free radicals within the polymerizing bone cement was examined using electron spin resonance spectroscopy. Severely compromised viability and completely suppressed phenotypes of osteoblasts on untreated bone cement were restored to the normal level by NAC incorporation. Bone volume formed around 25mm NAC-incorporated bone cement was threefold greater than that around control bone cement. The strength of bone-bone cement integration was 2.2 times greater for NAC-incorporated bone cement. For NAC-incorporated bone cement, the spike of free radical generation ended within 12h, whereas for control bone cement, a peak level lasted for 6 days and a level greater than half the level of the peak was sustained for 20 days. NAC also increased the level of antioxidant glutathione in osteoblasts. These results suggest that incorporation of NAC in PMMA bone cement detoxifies the material by immediate and effective in situ scavenging of free radicals and increasing intracellular antioxidant reserves, and consequently adds osteoconductivity to the material.

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

Members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on January 3, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. Shown here at MSFC's Manufacturing Engineering Laboratory are (left to right): Dr. Eberhard Rees, MSFC; Congressman George P. Miller, Democratic representative of California; Congressman F. Edward Hebert, Democratic representative of Louisiana; Congressman Robert R. Casey, Democratic representative of Texas; and Werner Kuers, MSFC.

Functional significance of O-GlcNAc modification in regulating neuronal properties.

PubMed

Hwang, Hongik; Rhim, Hyewhon

2018-03-01

Post-translational modifications (PTMs) covalently modify proteins and diversify protein functions. Along with protein phosphorylation, another common PTM is the addition of O-linked β-N-acetylglucosamine (O-GlcNAc) to serine and/or threonine residues. O-GlcNAc modification is similar to phosphorylation in that it occurs to serine and threonine residues and cycles on and off with a similar time scale. However, a striking difference is that the addition and removal of the O-GlcNAc moiety on all substrates are mediated by the two enzymes regardless of proteins, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA), respectively. O-GlcNAcylation can interact or potentially compete with phosphorylation on serine and threonine residues, and thus serves as an important molecular mechanism to modulate protein functions and activation. However, it has been challenging to address the role of O-GlcNAc modification in regulating protein functions at the molecular level due to the lack of convenient tools to determine the sites and degrees of O-GlcNAcylation. Studies in this field have only begun to expand significantly thanks to the recent advances in detection and manipulation methods such as quantitative proteomics and highly selective small-molecule inhibitors for OGT and OGA. Interestingly, multiple brain regions, especially hippocampus, express high levels of both OGT and OGA, and a number of neuron-specific proteins have been reported to undergo O-GlcNAcylation. This review aims to discuss the recent updates concerning the impacts of O-GlcNAc modification on neuronal functions at multiple levels ranging from intrinsic neuronal properties to synaptic plasticity and animal behaviors. Copyright © 2017 Elsevier Ltd. All rights reserved.

Dynamic interplay between catalytic and lectin domains of GalNAc-transferases modulates protein O-glycosylation

NASA Astrophysics Data System (ADS)

Lira-Navarrete, Erandi; de Las Rivas, Matilde; Compañón, Ismael; Pallarés, María Carmen; Kong, Yun; Iglesias-Fernández, Javier; Bernardes, Gonçalo J. L.; Peregrina, Jesús M.; Rovira, Carme; Bernadó, Pau; Bruscolini, Pierpaolo; Clausen, Henrik; Lostao, Anabel; Corzana, Francisco; Hurtado-Guerrero, Ramon

2015-05-01

Protein O-glycosylation is controlled by polypeptide GalNAc-transferases (GalNAc-Ts) that uniquely feature both a catalytic and lectin domain. The underlying molecular basis of how the lectin domains of GalNAc-Ts contribute to glycopeptide specificity and catalysis remains unclear. Here we present the first crystal structures of complexes of GalNAc-T2 with glycopeptides that together with enhanced sampling molecular dynamics simulations demonstrate a cooperative mechanism by which the lectin domain enables free acceptor sites binding of glycopeptides into the catalytic domain. Atomic force microscopy and small-angle X-ray scattering experiments further reveal a dynamic conformational landscape of GalNAc-T2 and a prominent role of compact structures that are both required for efficient catalysis. Our model indicates that the activity profile of GalNAc-T2 is dictated by conformational heterogeneity and relies on a flexible linker located between the catalytic and the lectin domains. Our results also shed light on how GalNAc-Ts generate dense decoration of proteins with O-glycans.

Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in Arabidopsis.

PubMed

Kim, Hyo Jung; Park, Ji-Hwan; Kim, Jingil; Kim, Jung Ju; Hong, Sunghyun; Kim, Jeongsik; Kim, Jin Hee; Woo, Hye Ryun; Hyeon, Changbong; Lim, Pyung Ok; Nam, Hong Gil; Hwang, Daehee

2018-05-22

Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in Arabidopsis during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a "NAC troika," govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other NAC s, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging. Copyright © 2018 the Author(s). Published by PNAS.

Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in Arabidopsis

PubMed Central

Kim, Hyo Jung; Park, Ji-Hwan; Kim, Jingil; Kim, Jung Ju; Hong, Sunghyun; Kim, Jin Hee; Woo, Hye Ryun; Lim, Pyung Ok; Nam, Hong Gil; Hwang, Daehee

2018-01-01

Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in Arabidopsis during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a “NAC troika,” govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other NACs, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging. PMID:29735710

Science: The Recombinant DNA Advisory Committee.

ERIC Educational Resources Information Center

Wright, Susan

1979-01-01

Reports on the status of the Recombinant DNA Advisory Committee (RAC) and attempts to rationalize Suburban Highway Policy. Effective communication among members of the RAC is a current problem facing the committee. A federal transportation priority spending policy is suggested during these times of money and fuel shortages. (MA)

The Novel Wheat Transcription Factor TaNAC47 Enhances Multiple Abiotic Stress Tolerances in Transgenic Plants

PubMed Central

Zhang, Lina; Zhang, Lichao; Xia, Chuan; Zhao, Guangyao; Jia, Jizeng; Kong, Xiuying

2016-01-01

NAC transcription factors play diverse roles in plant development and responses to abiotic stresses. However, the biological roles of NAC family members in wheat are not well understood. Here, we reported the isolation and functional characterization of a novel wheat TaNAC47 gene. TaNAC47 encoded protein, localizing in the nucleus, is able to bind to the ABRE cis-element and transactivate transcription in yeast, suggesting that it likely functions as a transcriptional activator. We also showed that TaNAC47 is differentially expressed in different tissues, and its expression was induced by the stress treatments of salt, cold, polyethylene glycol and exogenous abscisic acid. Furthermore, overexpression of TaNAC47 in Arabidopsis resulted in ABA hypersensitivity and enhancing tolerance of transgenic plants to drought, salt, and freezing stresses. Strikingly, overexpression of TaNAC47 was found to activate the expression of downstream genes and change several physiological indices that may enable transgenic plants to overcome unfavorable environments. Taken together, these results uncovered an important role of wheat TaNAC47 gene in response to ABA and abiotic stresses. PMID:26834757

The Novel Wheat Transcription Factor TaNAC47 Enhances Multiple Abiotic Stress Tolerances in Transgenic Plants.

PubMed

Zhang, Lina; Zhang, Lichao; Xia, Chuan; Zhao, Guangyao; Jia, Jizeng; Kong, Xiuying

2015-01-01

NAC transcription factors play diverse roles in plant development and responses to abiotic stresses. However, the biological roles of NAC family members in wheat are not well understood. Here, we reported the isolation and functional characterization of a novel wheat TaNAC47 gene. TaNAC47 encoded protein, localizing in the nucleus, is able to bind to the ABRE cis-element and transactivate transcription in yeast, suggesting that it likely functions as a transcriptional activator. We also showed that TaNAC47 is differentially expressed in different tissues, and its expression was induced by the stress treatments of salt, cold, polyethylene glycol and exogenous abscisic acid. Furthermore, overexpression of TaNAC47 in Arabidopsis resulted in ABA hypersensitivity and enhancing tolerance of transgenic plants to drought, salt, and freezing stresses. Strikingly, overexpression of TaNAC47 was found to activate the expression of downstream genes and change several physiological indices that may enable transgenic plants to overcome unfavorable environments. Taken together, these results uncovered an important role of wheat TaNAC47 gene in response to ABA and abiotic stresses.

A little sugar goes a long way: The cell biology of O-GlcNAc

PubMed Central

2015-01-01

Unlike the complex glycans decorating the cell surface, the O-linked β-N-acetyl glucosamine (O-GlcNAc) modification is a simple intracellular Ser/Thr-linked monosaccharide that is important for disease-relevant signaling and enzyme regulation. O-GlcNAcylation requires uridine diphosphate–GlcNAc, a precursor responsive to nutrient status and other environmental cues. Alternative splicing of the genes encoding the O-GlcNAc cycling enzymes O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) yields isoforms targeted to discrete sites in the nucleus, cytoplasm, and mitochondria. OGT and OGA also partner with cellular effectors and act in tandem with other posttranslational modifications. The enzymes of O-GlcNAc cycling act preferentially on intrinsically disordered domains of target proteins impacting transcription, metabolism, apoptosis, organelle biogenesis, and transport. PMID:25825515

76 FR 48901 - Office of Trade and Labor Affairs; National Advisory Committee for Labor Provisions of U.S. Free...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-09

...Pursuant to the Federal Advisory Committee Act (FACA), as amended, 5. U.S.C. App. 2, the Office of Trade and Labor Affairs (OTLA) gives notice of a meeting of the National Advisory Committee for Labor Provisions of U.S. Free Trade Agreements (``Committee'' or ``NAC''), which was established by the Secretary of Labor. The purpose of the meeting is to provide advice to the Secretary of Labor through the Bureau of International Labor Affairs (ILAB) concerning the implementation of the North American Agreement on Labor Cooperation (NAALC)--the labor side accord to the North American Free Trade Agreement (NAFTA)--and the labor provisions of free trade agreements.

Expression patterns and promoter analyses of aluminum-responsive NAC genes suggest a possible growth regulation of rice mediated by aluminum, hormones and NAC transcription factors.

PubMed

Escobar-Sepúlveda, Hugo Fernando; Trejo-Téllez, Libia Iris; García-Morales, Soledad; Gómez-Merino, Fernando Carlos

2017-01-01

In acid soils, the solubilized form of aluminum, Al+3, decreases root growth and affects the development of most crops. However, like other toxic elements, Al can have hormetic effects on plant metabolism. Rice (Oryza sativa) is one of the most tolerant species to Al toxicity, and when this element is supplied at low doses, growth stimulation has been observed, which could be due to combined mechanisms that are partly triggered by NAC transcription factors. This protein family can regulate vital processes in plants, including growth, development, and response to environmental stimuli, whether biotic or abiotic. Under our experimental conditions, 200 μM Al stimulated root growth and the formation of tillers; it also caused differential expression of a set of NAC genes. The promoter regions of the genes regulated by Al were analyzed and the cis-acting elements that are potentially involved in the responses to different stimuli, including environmental stress, were identified. Through the Genevestigator platform, data on the expression of NAC genes were obtained by experimental condition, tissue, and vegetative stage. This is the first study on NAC genes where in vivo and in silico data are complementarily analyzed, relating the hormetic effect of Al on plant growth and gene expression with a possible interaction in the response to phytohormones in rice. These findings could help to elucidate the possible convergence between the signaling pathways mediated by phytohormones and the role of the NAC transcription factors in the regulation of growth mediated by low Al doses.

Expression patterns and promoter analyses of aluminum-responsive NAC genes suggest a possible growth regulation of rice mediated by aluminum, hormones and NAC transcription factors

PubMed Central

2017-01-01

In acid soils, the solubilized form of aluminum, Al+3, decreases root growth and affects the development of most crops. However, like other toxic elements, Al can have hormetic effects on plant metabolism. Rice (Oryza sativa) is one of the most tolerant species to Al toxicity, and when this element is supplied at low doses, growth stimulation has been observed, which could be due to combined mechanisms that are partly triggered by NAC transcription factors. This protein family can regulate vital processes in plants, including growth, development, and response to environmental stimuli, whether biotic or abiotic. Under our experimental conditions, 200 μM Al stimulated root growth and the formation of tillers; it also caused differential expression of a set of NAC genes. The promoter regions of the genes regulated by Al were analyzed and the cis-acting elements that are potentially involved in the responses to different stimuli, including environmental stress, were identified. Through the Genevestigator platform, data on the expression of NAC genes were obtained by experimental condition, tissue, and vegetative stage. This is the first study on NAC genes where in vivo and in silico data are complementarily analyzed, relating the hormetic effect of Al on plant growth and gene expression with a possible interaction in the response to phytohormones in rice. These findings could help to elucidate the possible convergence between the signaling pathways mediated by phytohormones and the role of the NAC transcription factors in the regulation of growth mediated by low Al doses. PMID:29023561

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather first-hand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Marned Space Flight. Headed by Representative Olin E. Teague of Texas, other members were James G. Fulton, Pennsylvania; Ken Heckler, West Virginia; R. Walter Riehlman, New York; Richard L. Roudebush, Indiana; John W. Davis, Georgia; James C. Corman, California; Joseph Waggoner, Louisiana; J. Edgar Chenoweth, Colorado; and William G. Bray, Indiana.

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather first-hand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Marned Space Flight. Headed by Representative Olin E. Teague of Texas, other members were James G. Fulton, Pennsylvania; Ken Heckler, West Virginia; R. Walter Riehlman, New York; Richard L. Roudebush,, Indiana; John W. Davis, Georgia; James C. Corman, California; Joseph Waggoner, Louisiana; J. Edgar Chenoweth, Colorado; and William G. Bray, Indiana.

O-GlcNAc profiling: from proteins to proteomes

PubMed Central

2014-01-01

O-linked β-D-N-acetylglucosamine (O-GlcNAc) modification (O-GlcNAcylation) onto serine and threonine residues of proteins is an important post-translational modification (PTM), which is involved in many crucial biological processes including transcription, translation, proteasomal degradation, and signal transduction. Aberrant protein O-GlcNAcylation is directly linked to the pathological progression of chronic diseases including diabetes, cancer, and neurodegenerative disorders. Identification, site mapping, and quantification of O-GlcNAc proteins are a prerequisite to decipher their functions. In this review, we mainly focus on technological developments regarding O-GlcNAc protein profiling. Specifically, on one hand, we show how these techniques are being used for the comprehensive characterization of certain targeted proteins in which biologists are most interested. On the other hand, we present several newly developed approaches for O-GlcNAcomic profiling as well as how they provide us with a systems perspective to crosstalk amongst different PTMs and complicated biological events. Promising technical trends are also highlighted to evoke more efforts by diverse laboratories, which would further expand our understanding of the physiological and pathological roles of protein O-GlcNAcylation in chronic diseases. PMID:24593906

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. Standing at the Apollo Applications Program Cluster Model in building 4745 are (left-to-right): Dr. Wernher von Braun, MSFC; Congressman Joe D. Waggoner, Democratic representative of Louisiana; Congressman Earle Cabell, Democratic representative of Texas; Subcommittee Chairman Olin E. Teague, Democratic representative of Texas; Congressman James G. Fulton, Republican representative of Pennsylvania; and Dr. Ernst Stuhlinger, associate MSFC director for science. The subcommittee was briefed on MSFC's manned space efforts earlier in the day and then inspected mockups of the Saturn I Workshop and the Apollo Telescope Mount, two projects developed by MSFC for the post-Apollo program.

O-GlcNAc cycling: Emerging Roles in Development and Epigenetics

PubMed Central

Love, Dona C.; Krause, Michael W.; Hanover, John A.

2010-01-01

The nutrient-sensing hexosamine signaling pathway modulates the levels of O-linked N-acetylglucosamine (O-GlcNAc) on key targets impacting cellular signaling, protein turnover and gene expression. O-GlcNAc cycling may be deregulated in neurodegenerative disease, cancer, and diabetes. Studies in model organisms demonstrate that the O-GlcNAc transferase (OGT/Sxc) is essential for Polycomb group (PcG) repression of the homeotic genes, clusters of genes responsible for the adult body plan. Surprisingly, from flies to man, the O-GlcNAcase (OGA, MGEA5) gene is embedded within the NK cluster, the most evolutionarily ancient of three homeobox gene clusters regulated by PcG repression. PcG repression also plays a key role in maintaining stem cell identity, recruiting the DNA methyltransferase machinery for imprinting, and in X-chromosome inactivation. Intriguingly, the Ogt gene resides near the Xist locus in vertebrates and is subject to regulation by PcG-dependent X-inactivation. OGT is also an enzymatic component of the human dosage compensation complex. These ‘evo-devo’ relationships linking O-GlcNAc cycling to higher order chromatin structure provide insights into how nutrient availability may influence the epigenetic regulation of gene expression. O-GlcNAc cycling at promoters and PcG repression represent concrete mechanisms by which nutritional information may be transmitted across generations in the intra-uterine environment. Thus, the nutrient-sensing hexosamine signaling pathway may be a key contributor to the metabolic deregulation resulting from prenatal exposure to famine, or the ‘vicious cycle’ observed in children of mothers with type-2 diabetes and metabolic disease. PMID:20488252

Understanding the Role of O-GlcNAc Modifications in Plant Development

DOE Office of Scientific and Technical Information (OSTI.GOV)

Olszewski, Neil, E.

2011-06-16

This project has contributed towards understanding the role of O-GlcNAc (O-linked N-acetylglucosamine) transferases (OGTs) in plants. Through analyses of single and double mutants, we have investigated the unique and overlapping functions of SECRET AGENT (SEC) and SPINDLY (SPY), the arabidopsis OGTs. This work showed that SEC functions as negative regulators of the long-day flowering pathway. SEC also has a positive role in regulation of rosette. An E. coli co-expression system that allows potential substrates to be co-expressed with and O-GlcNAc modified by SEC was developed. We showed that SEC is a bona fide OGT that modifies itself with single O-linkedmore » GlcNAc(s). Using this system, we tested a number of proteins that were hypothesized to be substrates of SEC and identified a number of substrates include GIGANTEA (GI), a component of the long day flowering pathway. The hypothesis that O-GlcNAc modification controls GI activity was tested by first mapping where E. coli-expressed SEC modifies GI and then assessing the activity of a non-modifiable mutant form of GI. The activity of the mutant form of GI was indistinguishable from that of wild type suggesting that either O-GlcNAc does not regulate GI activity or that additional modification sites exist on GI. In collaboration with Dr. Juan Antonio Garcia at Universidad Autónoma de Madrid the role of O-GlcNAc modification of the plum pox virus coat protein (PPV-CP) was investigated. SEC was shown to O-GlcNAc modify PPV-CP and the modification was shown to facilitate the infection process. E. coli-expressed SEC was shown to modify the same PPV-CP sites that are modified in plants. SEC has a large protein interaction domain called the TPR domain that has been hypothesized to have a role in determining the substrate specificity of the enzyme and/or to regulate its activity. A mutational analysis of the TPR domain did not find evidence for a role in substrate specificity but did obtain evidence that the domain

Divergent and convergent synthesis of GalNAc-conjugated dendrimers using dual orthogonal ligations.

PubMed

Thomas, Baptiste; Pifferi, Carlo; Daskhan, Gour Chand; Fiore, Michele; Berthet, Nathalie; Renaudet, Olivier

2015-12-21

The synthesis of glycodendrimers remains a challenging task. In this paper we propose a protocol based on both oxime ligation (OL) to combine cyclopeptide repeating units as the dendritic core and the copper(i)-catalyzed azide-alkyne cycloaddition (CuAAC) to conjugate peripheral α and β propargylated GalNAc. By contrast with the oxime-based iterative protocol reported in our group, our current strategy can be used in both divergent and convergent routes with similar efficiency and the resulting hexadecavalent glycodendrimers can be easily characterized compared to oxime-linked analogues. A series of glycoconjugates displaying four or sixteen copies of both α and β GalNAc have been prepared and their ability to inhibit the adhesion of the soybean agglutinin (SBA) lectin to polymeric-GalNAc immobilized on microtiter plates has been evaluated. As was anticipated, the higher inhibitory effect (IC50 = 0.46 μM) was measured with the structure displaying αGalNAc with the higher valency (compound 13), which demonstrates that the binding properties of these glycoconjugates are strongly dependent on the orientation and distribution of the GalNAc units.

77 FR 55200 - Biological and Environmental Research Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-07

... News from the Biological Systems Science and Climate and Environmental Sciences Divisions Discussion of... DEPARTMENT OF ENERGY Biological and Environmental Research Advisory Committee AGENCY: Office of... the Biological and Environmental Research Advisory Committee (BERAC). The Federal Advisory Committee...

Genome-wide organization and expression profiling of the NAC transcription factor family in potato (Solanum tuberosum L.).

PubMed

Singh, Anil Kumar; Sharma, Vishal; Pal, Awadhesh Kumar; Acharya, Vishal; Ahuja, Paramvir Singh

2013-08-01

NAC [no apical meristem (NAM), Arabidopsis thaliana transcription activation factor [ATAF1/2] and cup-shaped cotyledon (CUC2)] proteins belong to one of the largest plant-specific transcription factor (TF) families and play important roles in plant development processes, response to biotic and abiotic cues and hormone signalling. Our genome-wide analysis identified 110 StNAC genes in potato encoding for 136 proteins, including 14 membrane-bound TFs. The physical map positions of StNAC genes on 12 potato chromosomes were non-random, and 40 genes were found to be distributed in 16 clusters. The StNAC proteins were phylogenetically clustered into 12 subgroups. Phylogenetic analysis of StNACs along with their Arabidopsis and rice counterparts divided these proteins into 18 subgroups. Our comparative analysis has also identified 36 putative TNAC proteins, which appear to be restricted to Solanaceae family. In silico expression analysis, using Illumina RNA-seq transcriptome data, revealed tissue-specific, biotic, abiotic stress and hormone-responsive expression profile of StNAC genes. Several StNAC genes, including StNAC072 and StNAC101that are orthologs of known stress-responsive Arabidopsis RESPONSIVE TO DEHYDRATION 26 (RD26) were identified as highly abiotic stress responsive. Quantitative real-time polymerase chain reaction analysis largely corroborated the expression profile of StNAC genes as revealed by the RNA-seq data. Taken together, this analysis indicates towards putative functions of several StNAC TFs, which will provide blue-print for their functional characterization and utilization in potato improvement.

Divergent Evolutionary Patterns of NAC Transcription Factors Are Associated with Diversification and Gene Duplications in Angiosperm

PubMed Central

Jin, Xiaoli; Ren, Jing; Nevo, Eviatar; Yin, Xuegui; Sun, Dongfa; Peng, Junhua

2017-01-01

NAC (NAM/ATAF/CUC) proteins constitute one of the biggest plant-specific transcription factor (TF) families and have crucial roles in diverse developmental programs during plant growth. Phylogenetic analyses have revealed both conserved and lineage-specific NAC subfamilies, among which various origins and distinct features were observed. It is reasonable to hypothesize that there should be divergent evolutionary patterns of NAC TFs both between dicots and monocots, and among NAC subfamilies. In this study, we compared the gene duplication and loss, evolutionary rate, and selective pattern among non-lineage specific NAC subfamilies, as well as those between dicots and monocots, through genome-wide analyses of sequence and functional data in six dicot and five grass lineages. The number of genes gained in the dicot lineages was much larger than that in the grass lineages, while fewer gene losses were observed in the grass than that in the dicots. We revealed (1) uneven constitution of Clusters of Orthologous Groups (COGs) and contrasting birth/death rates among subfamilies, and (2) two distinct evolutionary scenarios of NAC TFs between dicots and grasses. Our results demonstrated that relaxed selection, resulting from concerted gene duplications, may have permitted substitutions responsible for functional divergence of NAC genes into new lineages. The underlying mechanism of distinct evolutionary fates of NAC TFs shed lights on how evolutionary divergence contributes to differences in establishing NAC gene subfamilies and thus impacts the distinct features between dicots and grasses. PMID:28713414

77 FR 12086 - Committee Management Renewals

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

... NATIONAL SCIENCE FOUNDATION Committee Management Renewals The NSF management officials having... follows consultation with the Committee Management Secretariat, General Services Administration... Bolton, NSF, at (703) 292-7488. Dated: February 22, 2012. Susanne Bolton, Committee Management Officer...

Report: EPA Has Adequate Controls to Manage Advice From Science and Research Federal Advisory Committees, but Transparency Could Be Improved

EPA Pesticide Factsheets

Report #17-P-0124, March 13, 2017. Science plays an integral role in the EPA's mission. The EPA has an adequate system of controls to manage recommendations from its science and research federal advisory committees.

N-Acetylcysteine (NAC)-Induced Hyponatremia Caused by an Electronic Medical Record (EMR) Order Error.

PubMed

Furmaga, Jakub; Wax, Paul; Kleinschmidt, Kurt

2015-09-01

Intravenous N-acetylcysteine (NAC) causes few adverse drug events, with mild anaphylactoid reactions being the most common. Hyponatremia as a complication of hypoosmolar NAC solution has been reported. We describe how a locally constructed electronic medical record (EMR) order set for IV NAC resulted in a seizure from hyponatremia due to excess free water administration. A 13-month-old female with no past medical history presented to a hospital after ingesting an unknown number of acetaminophen 500 mg tablets. The 4-h acetaminophen concentration was 343 mcg/mL, and she was started on IV NAC. 8.2 h into her 21-h IV NAC protocol, she developed a tonic-clonic seizure. Repeat serum sodium was 124 mEq/L, a decrease from 142 mEq/L at the time of admission. She was treated with hypertonic saline, lorazepam, and levetiracetam and had no further seizures. A brain MRI and EEG were both normal. After the seizure was stabilized, the providers noticed that the patient had receive a total of 900 mL of D5W (112.5 mL/kg) in the first 9 h of hospitalization. This was caused by a poorly constructed, restrictive, EMR order set that did not allow customization of the IV NAC preparation. Because the 21-h IV NAC administration involves preparation of 3 different doses infused over 3 different time intervals, an order set was developed to reduce ordering errors. However, error in its construction caused the pharmacist to prepare a solution containing too much free water, decreasing patient's intravascular sodium and resulting in a seizure. The purposes of our case report were to highlight the dangers of overreliance on EMR order sets and to recognize hyponatremic seizures as an adverse reaction of an inappropriately prepared IV NAC.

O-GlcNAc and the Cardiovascular System

PubMed Central

Dassanayaka, Sujith; Jones, Steven P.

2014-01-01

The cardiovascular system is capable of robust changes in response to physiologic and pathologic stimuli through intricate signaling mechanisms. The area of metabolism has witnessed a veritable renaissance in the cardiovascular system. In particular, the post-translational β-O-linkage of N-acetylglucosamine (O-GlcNAc) to cellular proteins represents one such signaling pathway that has been implicated in the pathophysiology of cardiovascular disease. This highly dynamic protein modification may induce functional changes in proteins and regulate key cellular processes including translation, transcription, and cell death. In addition, its potential interplay with phosphorylation provides an additional layer of complexity to post-translational regulation. The hexosamine biosynthetic pathway generally requires glucose to form the nucleotide sugar, UDP-GlcNAc. Accordingly, O-GlcNAcylation may be altered in response to nutrient availability and cellular stress. Recent literature supports O-GlcNAcylation as an autoprotective response in models of acute stress (hypoxia, ischemia, oxidative stress). Models of sustained stress, such as pressure overload hypertrophy, and infarct-induced heart failure, may also require protein O-GlcNAcylation as a partial compensatory mechanism. Yet, in models of Type II diabetes, O-GlcNAcylation has been implicated in the subsequent development of vascular, and even cardiac, dysfunction. This review will address this apparent paradox and discuss the potential mechanisms of O-GlcNAc-mediated cardioprotection and cardiovascular dysfunction. This discussion will also address potential targets for pharmacologic interventions and the unique considerations related to such targets. PMID:24287310

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. The subcommittee was briefed on MSFC's manned space efforts earlier in the day and then inspected mockups of the Saturn I Workshop and the Apollo Telescope Mount, two projects developed by MSFC for the post-Apollo program. In this photograph, MSFC Director, Dr. Wernher von Braun and Richard L. Roudebush, Republican representative of Indiana, discuss Apollo models.

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. The subcommittee was briefed on MSFC's manned space efforts earlier in the day and then inspected mockups of the Saturn I Workshop and the Apollo Telescope Mount, two projects developed by MSFC for the post-Apollo program. In this photograph, MSFC Director, Dr. Wernher von Braun and Joe Waggoner, Democratic representative of Louisiana, discuss Apollo models.

In Vitro Biosynthesis and Chemical Identification of UDP-N-acetyl-d-quinovosamine (UDP-d-QuiNAc)*

PubMed Central

Li, Tiezheng; Simonds, Laurie; Kovrigin, Evgenii L.; Noel, K. Dale

2014-01-01

N-acetyl-d-quinovosamine (2-acetamido-2,6-dideoxy-d-glucose, QuiNAc) occurs in the polysaccharide structures of many Gram-negative bacteria. In the biosynthesis of QuiNAc-containing polysaccharides, UDP-QuiNAc is the hypothetical donor of the QuiNAc residue. Biosynthesis of UDP-QuiNAc has been proposed to occur by 4,6-dehydration of UDP-N-acetyl-d-glucosamine (UDP-GlcNAc) to UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulose followed by reduction of this 4-keto intermediate to UDP-QuiNAc. Several specific dehydratases are known to catalyze the first proposed step. A specific reductase for the last step has not been demonstrated in vitro, but previous mutant analysis suggested that Rhizobium etli gene wreQ might encode this reductase. Therefore, this gene was cloned and expressed in Escherichia coli, and the resulting His6-tagged WreQ protein was purified. It was tested for 4-reductase activity by adding it and NAD(P)H to reaction mixtures in which 4,6-dehydratase WbpM had acted on the precursor substrate UDP-GlcNAc. Thin layer chromatography of the nucleotide sugars in the mixture at various stages of the reaction showed that WbpM converted UDP-GlcNAc completely to what was shown to be its 4-keto-6-deoxy derivative by NMR and that addition of WreQ and NADH led to formation of a third compound. Combined gas chromatography-mass spectrometry analysis of acid hydrolysates of the final reaction mixture showed that a quinovosamine moiety had been synthesized after WreQ addition. The two-step reaction progress also was monitored in real time by NMR. The final UDP-sugar product after WreQ addition was purified and determined to be UDP-d-QuiNAc by one-dimensional and two-dimensional NMR experiments. These results confirmed that WreQ has UDP-2-acetamido-2,6-dideoxy-d-xylo-4-hexulose 4-reductase activity, completing a pathway for UDP-d-QuiNAc synthesis in vitro. PMID:24817117

75 FR 9616 - Committee Management Renewals

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-03

... NATIONAL SCIENCE FOUNDATION Committee Management Renewals The NSF management officials having... follows consultation with the Committee Management Secretariat, General Services Administration... Bolton, NSF, at (703) 292-7488. Susanne Bolton, Committee Management Officer. [FR Doc. 2010-4233 Filed 3...

Report of the Fusion Energy Sciences Advisory Committee Panel on Priorities and Balance

NASA Astrophysics Data System (ADS)

Baker, Charles; Davidson, Ronald; Dean, Stephen; Freidberg, Jeffrey; Sheffield, John

1999-06-01

This report presents the results and recommendations of the deliberations of the DOE Fusion Energy Sciences Advisory Committee (FESAC) Panel on Priorities and Balance, which met in Knoxville, TN, 18-21 August 1999. The Panel identified the achievement of a more integrated national program in magnetic fusion energy (MFE) and inertial fusion energy (IFE) as a major programmatic and policy goal for the years ahead.

76 FR 20720 - Committee Management; Renewals

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-13

... NATIONAL SCIENCE FOUNDATION Committee Management; Renewals The NSF management officials having.... 1861 et seq. This determination follows consultation with the Committee Management Secretariat, General... Susanne Bolton, NSF, at (703) 292-7488. Dated: April 8, 2011. Susanne Bolton, Committee Management Officer...

75 FR 36698 - Committee Management Renewals

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-28

... NATIONAL SCIENCE FOUNDATION Committee Management Renewals The NSF management officials having... follows consultation with the Committee Management Secretariat, General Services Administration..., Committee Management Officer. [FR Doc. 2010-15565 Filed 6-25-10; 8:45 am] BILLING CODE 7555-01-P ...

78 FR 21979 - Committee Management; Renewal

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-12

... NATIONAL SCIENCE FOUNDATION Committee Management; Renewal The NSF management officials having.... 1861 et seq. This determination follows consultation with the Committee Management Secretariat, General... Susanne Bolton, NSF, at (703) 292-7488. Dated: April 8, 2013. Susanne Bolton, Committee Management Officer...

New ELISA-based method for the detection of O-GlcNAc transferase activity in vitro.

PubMed

Qi, Jieqiong; Wang, Ruihong; Zeng, Yazhen; Yu, Wengong; Gu, Yuchao

2017-08-09

O-GlcNAcylation is a dynamic, reversible, post-translational modification that regulates many cellular processes. O-GlcNAc transferase (OGT) is the sole enzyme transferring N-acetylglucosamine from uridine diphosphate (UDP)-GlcNAc to selected serine/threonine residues of cytoplasm and nucleus proteins. Aberrant of OGT activity is associated with several diseases, suggesting OGT as a novel therapeutic target. In this study, we created a new enzyme linked immunosorbent assays (ELISA)-based method for detection of OGT activity. First, casein kinase II (CKII), a well-known OGT substrate, was coated onto ELISA plate. Second, the GlcNAc transferred by OGT from UDP-GlcNAc to CKII was detected using an antibody to O-GlcNAc and then the horseradish peroxidase (HRP)-labeled secondary antibody. At last, 3,3',5,5'-tetramethylbenzidine (TMB), the substrate of HRP, was used to detect the O-GlcNAcylation level of CKII which reflected the activity of OGT. Based on a series of optimization experiments, the RL2 antibody was selected for O-GlcNAc detection and the concentrations of CKII, OGT, and UDP-GlcNAc were determined in this study. ST045849, a commercial OGT inhibitor, was used to verify the functionality of the system. Altogether, this study showed a method that could be applied to detect OGT activity and screen OGT inhibitors.

Glycoprotein synthesis in yeast. Identification of Man8GlcNAc2 as an essential intermediate in oligosaccharide processing.

PubMed

Byrd, J C; Tarentino, A L; Maley, F; Atkinson, P H; Trimble, R B

1982-12-25

Synthesis of the N-linked oligosaccharides of Saccharomyces cerevisiae glycoproteins has been studied in vivo by labeling with [2-3H]mannose and gel filtration analysis of the products released by endoglycosidase H. Both small oligosaccharides, Man8-14GlcNAc, and larger products, Man greater than 20GlcNAc, were labeled. The kinetics of continuous and pulse-chase labeling demonstrated that Glc3Man9GlcNAc2, the initial product transferred to protein, was rapidly (t1/2 congruent to 3 min) trimmed to Man8GlcNAc2 and then more slowly (t1/2 = 10-20 min) elongated to larger oligosaccharides. No oligosaccharides smaller than Man8GlcNAc2 were evident with either labeling procedure. In confirmation of the trimming reaction observed in vivo, 3H-labeled Man9-N-acetylglucosaminitol from bovine thyroglobulin and [14C]Man9GlcNAc2 from yeast oligosaccharide-lipid were converted in vitro by broken yeast cells to 3H-labeled Man8-N-acetylglucosaminitol and [14C]Man8GlcNAc2. Man8GlcNAc and Man9GlcNAc from yeast invertase and from bovine thyroglobulin were purified by gel filtration and examined by high field 1H-NMR analysis. Invertase Man8GlcNAc (B) and Man9GlcNAc (C) were homogeneous compounds, which differed from the Man9GlcNAc (A) of thyroglobulin by the absence of a specific terminal alpha 1,2-linked mannose residue. The Man9GlcNAc of invertase (C) had an additional terminal alpha 1,6-linked mannose and appeared identical in structure with that isolated from yeast containing the mnn1 and mnn2 mutations (Cohen, R. E., Zhang, W.-j., and Ballou, C. E. (1982) J. Biol. Chem. 257, 5730-5737). It is concluded that Man8GlcNAc2, formed by removal of glucose and a single mannose from Glc3Man9GlcNAc2, is the ultimate product of trimming and the minimal precursor for elongation of the oligosaccharides on yeast glycoproteins. The results suggest that removal of a particular terminal alpha 1,2-linked mannose from Man9GlcNAc2 by a highly specific alpha-mannosidase exposes the nascent Man-alpha 1

Phosphorylation of a NAC Transcription Factor by a Calcium/Calmodulin-Dependent Protein Kinase Regulates Abscisic Acid-Induced Antioxidant Defense in Maize [Phosphorylation of a NAC Transcription Factor by ZmCCaMK Regulates Abscisic Acid-Induced Antioxidant Defense in Maize

DOE PAGES

Zhu, Yuan; Yan, Jingwei; Liu, Weijuan; ...

2016-05-10

Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play an important role in abscisic acid (ABA)-induced antioxidant defense and enhance the tolerance of plants to drought stress. However, its downstream molecular events are poorly understood. Here, we identify a NAC transcription factor, ZmNAC84, in maize, which physically interacts with ZmCCaMK in vitro and in vivo. ZmNAC84 display a partially overlapping expression pattern with ZmCCaMK after ABA treatment and H 2O 2 is required for ABA-induced ZmNAC84 expression. Functional analysis reveals that ZmNAC84 is essential for ABA-induced antioxidant defense in a ZmCCaMK-dependent manner. Furthermore, ZmCCaMK directly phosphorylates S113 of ZmNAC84 inmore » vitro, and S113 is essential for the ABA-induced stimulation of antioxidant defense by ZmCCaMK. Moreover, overexpression of ZmNAC84 in tobacco can improve drought tolerance, and alleviate drought-induced oxidative damage of transgenic plants. These results define a mechanism for ZmCCaMK function in ABA-induced antioxidant defense, where ABA-produced H 2O 2 first induces expression of ZmCCaMK and ZmNAC84 and activates ZmCCaMK, and subsequently the activated ZmCCaMK phosphorylates ZmNAC84 at S113, thereby inducing antioxidant defense by activating downstream genes.« less

Phosphorylation of a NAC Transcription Factor by a Calcium/Calmodulin-Dependent Protein Kinase Regulates Abscisic Acid-Induced Antioxidant Defense in Maize [Phosphorylation of a NAC Transcription Factor by ZmCCaMK Regulates Abscisic Acid-Induced Antioxidant Defense in Maize

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhu, Yuan; Yan, Jingwei; Liu, Weijuan

Calcium/calmodulin-dependent protein kinase (CCaMK) has been shown to play an important role in abscisic acid (ABA)-induced antioxidant defense and enhance the tolerance of plants to drought stress. However, its downstream molecular events are poorly understood. Here, we identify a NAC transcription factor, ZmNAC84, in maize, which physically interacts with ZmCCaMK in vitro and in vivo. ZmNAC84 display a partially overlapping expression pattern with ZmCCaMK after ABA treatment and H 2O 2 is required for ABA-induced ZmNAC84 expression. Functional analysis reveals that ZmNAC84 is essential for ABA-induced antioxidant defense in a ZmCCaMK-dependent manner. Furthermore, ZmCCaMK directly phosphorylates S113 of ZmNAC84 inmore » vitro, and S113 is essential for the ABA-induced stimulation of antioxidant defense by ZmCCaMK. Moreover, overexpression of ZmNAC84 in tobacco can improve drought tolerance, and alleviate drought-induced oxidative damage of transgenic plants. These results define a mechanism for ZmCCaMK function in ABA-induced antioxidant defense, where ABA-produced H 2O 2 first induces expression of ZmCCaMK and ZmNAC84 and activates ZmCCaMK, and subsequently the activated ZmCCaMK phosphorylates ZmNAC84 at S113, thereby inducing antioxidant defense by activating downstream genes.« less

Climate Change Adaptation Act : report of the Committee on Commerce, Science, and Transportation on S. 2355

DOT National Transportation Integrated Search

2008-06-05

The Committee on Commerce, Science, and Transportation, to which was referred the bill (S. 2355) to amend the National Climate Program Act to enhance the ability of the United States to : develop and implement climate change adaptation programs and p...

Report on Computing and Networking in the Space Science Laboratory by the SSL Computer Committee

NASA Technical Reports Server (NTRS)

Gallagher, D. L. (Editor)

1993-01-01

The Space Science Laboratory (SSL) at Marshall Space Flight Center is a multiprogram facility. Scientific research is conducted in four discipline areas: earth science and applications, solar-terrestrial physics, astrophysics, and microgravity science and applications. Representatives from each of these discipline areas participate in a Laboratory computer requirements committee, which developed this document. The purpose is to establish and discuss Laboratory objectives for computing and networking in support of science. The purpose is also to lay the foundation for a collective, multiprogram approach to providing these services. Special recognition is given to the importance of the national and international efforts of our research communities toward the development of interoperable, network-based computer applications.

O-GlcNAc transferase inhibitors: current tools and future challenges.

PubMed

Trapannone, Riccardo; Rafie, Karim; van Aalten, Daan M F

2016-02-01

The O-linked N-acetylglucosamine (O-GlcNAc) post-translational modification (O-GlcNAcylation) is the dynamic and reversible attachment of N-acetylglucosamine to serine and threonine residues of nucleocytoplasmic target proteins. It is abundant in metazoa, involving hundreds of proteins linked to a plethora of biological functions with implications in human diseases. The process is catalysed by two enzymes: O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) that add and remove sugar moieties respectively. OGT knockout is embryonic lethal in a range of animal models, hampering the study of the biological role of O-GlcNAc and the dissection of catalytic compared with non-catalytic roles of OGT. Therefore, selective and potent chemical tools are necessary to inhibit OGT activity in the context of biological systems. The present review focuses on the available OGT inhibitors and summarizes advantages, limitations and future challenges. © 2016 Authors; published by Portland Press Limited.

This far and no further: The rise and fall of the committee on earth and environmental sciences

DOE Office of Scientific and Technical Information (OSTI.GOV)

Delwiche, C.C.

1993-08-10

During the 1970s and 1980s, issues of global change-zone depletion, global warming, deforestation, and more-captured both the popular and scientific imagination. Scientists in specialties like oceanography and climatology developed new, interdisciplinary approaches reflecting the complex processes involved, and launched a major international initiative, the International Geosphere-Biosphere Program (ICBP), linking dozens of countries` research efforts. In the United States, federal agencies involved in earth science research, particularly the National Aeronautics and Space Administration (NASA), the National Oceanic and Atmospheric Administration (NOAA), and the National Science Foundation (NSF), began programs in global change, and also began to struggle among themselves for bureaucraticmore » preeminence in the new area. At the same time, the Office of Science and Technology Policy (OSTP) and the Office of Management and Budget (OMB), both part of the Executive Office of the President, began to worry that federal agencies` various global change activities were not well planned and coordinated, and that the country was not getting as much for its money as it could be. In 1987, William R. Graham, director of OSTP, created the Committee on Earth Sciences (CES) under the Federal Coordinating Council on Science, Engineering and Technology (FCCSET) to improve the situation. Chaired by NOAA chief Tony Calio its charge was to plan and coordinate the goverment`s global change research and provide scientific and policy advice to the rest of the government. The CES`s first meeting was a disaster. The agency representatives refused to accept Calio`s leadership and were bluntly told by OMB not to expect new funding for global change research. Calio left both the committee and the government. Graham was faced with the choice of abandoning the new committee or taking steps to launch it anew.« less

An OGA-Resistant Probe Allows Specific Visualization and Accurate Identification of O-GlcNAc-Modified Proteins in Cells.

PubMed

Li, Jing; Wang, Jiajia; Wen, Liuqing; Zhu, He; Li, Shanshan; Huang, Kenneth; Jiang, Kuan; Li, Xu; Ma, Cheng; Qu, Jingyao; Parameswaran, Aishwarya; Song, Jing; Zhao, Wei; Wang, Peng George

2016-11-18

O-linked β-N-acetyl-glucosamine (O-GlcNAc) is an essential and ubiquitous post-translational modification present in nucleic and cytoplasmic proteins of multicellular eukaryotes. The metabolic chemical probes such as GlcNAc or GalNAc analogues bearing ketone or azide handles, in conjunction with bioorthogonal reactions, provide a powerful approach for detecting and identifying this modification. However, these chemical probes either enter multiple glycosylation pathways or have low labeling efficiency. Therefore, selective and potent probes are needed to assess this modification. We report here the development of a novel probe, 1,3,6-tri-O-acetyl-2-azidoacetamido-2,4-dideoxy-d-glucopyranose (Ac 3 4dGlcNAz), that can be processed by the GalNAc salvage pathway and transferred by O-GlcNAc transferase (OGT) to O-GlcNAc proteins. Due to the absence of a hydroxyl group at C4, this probe is less incorporated into α/β 4-GlcNAc or GalNAc containing glycoconjugates. Furthermore, the O-4dGlcNAz modification was resistant to the hydrolysis of O-GlcNAcase (OGA), which greatly enhanced the efficiency of incorporation for O-GlcNAcylation. Combined with a click reaction, Ac 3 4dGlcNAz allowed the selective visualization of O-GlcNAc in cells and accurate identification of O-GlcNAc-modified proteins with LC-MS/MS. This probe represents a more potent and selective tool in tracking, capturing, and identifying O-GlcNAc-modified proteins in cells and cell lysates.

O-GlcNAc and the cardiovascular system.

PubMed

Dassanayaka, Sujith; Jones, Steven P

2014-04-01

The cardiovascular system is capable of robust changes in response to physiologic and pathologic stimuli through intricate signaling mechanisms. The area of metabolism has witnessed a veritable renaissance in the cardiovascular system. In particular, the post-translational β-O-linkage of N-acetylglucosamine (O-GlcNAc) to cellular proteins represents one such signaling pathway that has been implicated in the pathophysiology of cardiovascular disease. This highly dynamic protein modification may induce functional changes in proteins and regulate key cellular processes including translation, transcription, and cell death. In addition, its potential interplay with phosphorylation provides an additional layer of complexity to post-translational regulation. The hexosamine biosynthetic pathway generally requires glucose to form the nucleotide sugar, UDP-GlcNAc. Accordingly, O-GlcNAcylation may be altered in response to nutrient availability and cellular stress. Recent literature supports O-GlcNAcylation as an autoprotective response in models of acute stress (hypoxia, ischemia, oxidative stress). Models of sustained stress, such as pressure overload hypertrophy, and infarct-induced heart failure, may also require protein O-GlcNAcylation as a partial compensatory mechanism. Yet, in models of Type II diabetes, O-GlcNAcylation has been implicated in the subsequent development of vascular, and even cardiac, dysfunction. This review will address this apparent paradox and discuss the potential mechanisms of O-GlcNAc-mediated cardioprotection and cardiovascular dysfunction. This discussion will also address potential targets for pharmacologic interventions and the unique considerations related to such targets. Copyright © 2013 Elsevier Inc. All rights reserved.

The role of O-GlcNAc signaling in the pathogenesis of diabetic retinopathy.

PubMed

Semba, Richard D; Huang, Hu; Lutty, Gerard A; Van Eyk, Jennifer E; Hart, Gerald W

2014-04-01

Diabetic retinopathy is a leading cause of blindness worldwide. Despite laser and surgical treatments, antiangiogenic and other therapies, and strict metabolic control, many patients progress to visual impairment and blindness. New insights are needed into the pathophysiology of diabetic retinopathy in order to develop new methods to improve the detection and treatment of disease and the prevention of blindness. Hyperglycemia and diabetes result in increased flux through the hexosamine biosynthetic pathway, which, in turn, results in increased PTM of Ser/Thr residues of proteins by O-linked β-N-acetylglucosamine (O-GlcNAc). O-GlcNAcylation is involved in regulation of many nuclear and cytoplasmic proteins in a manner similar to protein phosphorylation. Altered O-GlcNAc signaling has been implicated in the pathogenesis of diabetes and may play an important role in the pathogenesis of diabetic retinopathy. The goal of this review is to summarize the biology of the hexosamine biosynthesis pathway and O-GlcNAc signaling, to present the current evidence for the role of O-GlcNAc signaling in diabetes and diabetic retinopathy, and to discuss future directions for research on O-GlcNAc in the pathogenesis of diabetic retinopathy. © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Child at Risk: A Report of the Standing Senate Committee on Health, Welfare and Science.

ERIC Educational Resources Information Center

Senate Committee of Canada (Ontario). Standing Senate Committee on Health, Welfare and Science.

This report of the Standing Senate Committee on Health, Welfare and Science summarizes and coordinates interdisciplinary evidence and expert opinion in order to provide insight into the way early childhood experiences might be considered as causes of later criminal behavior. The material is divided into three categories: the prenatal period, the…

Nac1 promotes self-renewal of embryonic stem cells through direct transcriptional regulation of c-Myc.

PubMed

Ruan, Yan; He, Jianrong; Wu, Wei; He, Ping; Tian, Yanping; Xiao, Lan; Liu, Gaoke; Wang, Jiali; Cheng, Yuda; Zhang, Shuo; Yang, Yi; Xiong, Jiaxiang; Zhao, Ke; Wan, Ying; Huang, He; Zhang, Junlei; Jian, Rui

2017-07-18

The pluripotency transcriptional network in embryonic stem cells (ESCs) is composed of distinct functional units including the core and Myc units. It is hoped that dissection of the cellular functions and interconnections of network factors will aid our understanding of ESC and cancer biology. Proteomic and genomic approaches have identified Nac1 as a member of the core pluripotency network. However, previous studies have predominantly focused on the role of Nac1 in psychomotor stimulant response and cancer pathogenesis. In this study, we report that Nac1 is a self-renewal promoting factor, but is not required for maintaining pluripotency of ESCs. Loss of function of Nac1 in ESCs results in a reduced proliferation rate and an enhanced differentiation propensity. Nac1 overexpression promotes ESC proliferation and delays ESC differentiation in the absence of leukemia inhibitory factor (LIF). Furthermore, we demonstrated that Nac1 directly binds to the c-Myc promoter and regulates c-Myc transcription. The study also revealed that the function of Nac1 in promoting ESC self-renewal appears to be partially mediated by c-Myc. These findings establish a functional link between the core and c-Myc-centered networks and provide new insights into mechanisms of stemness regulation in ESCs and cancer.

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. The subcommittee was briefed on MSFC's manned space efforts earlier in the day and then inspected mockups of the Saturn I Workshop and the Apollo Telescope Mount, two projects developed by MSFC for the post-Apollo program. In this photograph, MSFC Director, Dr. Wernher von Braun, bids farewell to Texas Democratic Representative Olin E. Teague before departure at the Redstone Arsenal Airstrip.

Committee on Military Nutrition Research

DTIC Science & Technology

2007-01-01

AD_________________ Award Number: DAMD17-99-1-9478 TITLE: COMMITTEE ON MILITARY NUTRITION ...COVERED (From - To) 01 Jun 99 – 31 Dec 06 4. TITLE AND SUBTITLE COMMITTEE ON MILITARY NUTRITION RESEARCH 5a. CONTRACT NUMBER...report presents the activities of the National Academy of Sciences/Institute of Medicine’s Committee on Military Nutrition Research (CMNR) for the

The effect of N-acetyl-l-cysteine (NAC) on liver toxicity and clinical outcome after hematopoietic stem cell transplantation.

PubMed

El-Serafi, Ibrahim; Remberger, Mats; El-Serafi, Ahmed; Benkessou, Fadwa; Zheng, Wenyi; Martell, Eva; Ljungman, Per; Mattsson, Jonas; Hassan, Moustapha

2018-05-29

Busulphan (Bu) is a myeloablative drug used for conditioning prior to hematopoietic stem cell transplantation. Bu is predominantly metabolized through glutathione conjugation, a reaction that consumes the hepatic glutathione. N-acetyl-l-cysteine (NAC) is a glutathione precursor used in the treatment of acetaminophen hepatotoxicity. NAC does not interfere with the busulphan myeloablative effect. We investigated the effect of NAC concomitant treatment during busulphan conditioning on the liver enzymes as well as the clinical outcome. Prophylactic NAC treatment was given to 54 patients upon the start of busulphan conditioning. These patients were compared with 54 historical matched controls who did not receive NAC treatment. In patients treated with NAC, aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (ALP) were significantly (P < 0.05) decreased after conditioning compared to their start values. Within the NAC-group, liver enzymes were normalized in those patients (30%) who had significantly high start values. No significant decrease in enzyme levels was observed in the control group. Furthermore, NAC affected neither Bu kinetics nor clinical outcome (sinusoidal obstruction syndrome incidence, graft-versus-host disease and/or graft failure). NAC is a potential prophylactic treatment for hepatotoxicity during busulphan conditioning. NAC therapy did not alter busulphan kinetics or affect clinical outcome.

A transposable element in a NAC gene is associated with drought tolerance in maize seedlings

PubMed Central

Mao, Hude; Wang, Hongwei; Liu, Shengxue; Li, Zhigang; Yang, Xiaohong; Yan, Jianbing; Li, Jiansheng; Tran, Lam-Son Phan; Qin, Feng

2015-01-01

Drought represents a major constraint on maize production worldwide. Understanding the genetic basis for natural variation in drought tolerance of maize may facilitate efforts to improve this trait in cultivated germplasm. Here, using a genome-wide association study, we show that a miniature inverted-repeat transposable element (MITE) inserted in the promoter of a NAC gene (ZmNAC111) is significantly associated with natural variation in maize drought tolerance. The 82-bp MITE represses ZmNAC111 expression via RNA-directed DNA methylation and H3K9 dimethylation when heterologously expressed in Arabidopsis. Increasing ZmNAC111 expression in transgenic maize enhances drought tolerance at the seedling stage, improves water-use efficiency and induces upregulation of drought-responsive genes under water stress. The MITE insertion in the ZmNAC111 promoter appears to have occurred after maize domestication and spread among temperate germplasm. The identification of this MITE insertion provides insight into the genetic basis for natural variation in maize drought tolerance. PMID:26387805

Poly-LacNAc as an Age-Specific Ligand for Rotavirus P[11] in Neonates and Infants

PubMed Central

Liu, Yang; Huang, Pengwei; Jiang, Baoming; Tan, Ming; Morrow, Ardythe L.; Jiang, Xi

2013-01-01

Rotavirus (RV) P[11] is an unique genotype that infects neonates. The mechanism of such age-specific host restriction remains unknown. In this study, we explored host mucosal glycans as a potential age-specific factor for attachment of P[11] RVs. Using in vitro binding assays, we demonstrated that VP8* of a P[11] RV (N155) could bind saliva of infants (60.3%, N = 151) but not of adults (0%, N = 48), with a significantly negative correlation between binding of VP8* and ages of infants (P<0.01). Recognition to the infant saliva did not correlate with the ABO, secretor and Lewis histo-blood group antigens (HBGAs) but with the binding of the lectin Lycopersicon esculentum (LEA) that is known to recognize the oligomers of N-acetyllactosamine (LacNAc), a precursor of human HBGAs. Direct evidence of LacNAc involvement in P[11] binding was obtained from specific binding of VP8* with homopolymers of LacNAc in variable lengths through a glycan array analysis of 611 glycans. These results were confirmed by strong binding of VP8* to the Lec2 cell line that expresses LacNAc oligomers but not to the Lec8 cell line lacking the LacNAc. In addition, N155 VP8* and authentic P[11] RVs (human 116E and bovine B223) hemagglutinated human red blood cells that are known to express poly-LacNAc. The potential role of poly-LacNAc in host attachment and infection of RVs has been obtained by abrogation of 116E replication by the PAA-conjugated poly-LacNAc, human milk, and LEA positive infant saliva. Overall, our results suggested that the poly-LacNAc could serve as an age-specific receptor for P[11] RVs and well explained the epidemiology that P[11] RVs mainly infect neonates and young children. PMID:24244290

Protein O-GlcNAc Modification Increases in White Blood Cells After a Single Bout of Physical Exercise.

PubMed

Nagy, Tamás; Kátai, Emese; Fisi, Viktória; Takács, Tamás Tibor; Stréda, Antal; Wittmann, István; Miseta, Attila

2018-01-01

Protein O-linked N -acetylglucosamine (O-GlcNAc) is a dynamic posttranslational modification influencing the function of many intracellular proteins. Recently it was revealed that O-GlcNAc regulation is modified under various stress states, including ischemia and oxidative stress. Aside from a few contradictory studies based on animal models, the effect of exercise on O-GlcNAc is unexplored. To evaluate O-GlcNAc levels in white blood cells (WBC) of human volunteers following physical exercise. Young (age 30 ± 5.2), healthy male volunteers ( n  = 6) were enlisted for the study. Blood parameters including metabolites, ions, "necro"-enzymes, and cell counts were measured before and after a single bout of exercise (2-mile run). From WBC samples, we performed western blots to detect O-GlcNAc modified proteins. The distribution of O-GlcNAc in WBC subpopulations was assessed by flow cytometry. Elevation of serum lactic acid (increased from 1.3 ± 0.4 to 6.9 ± 1.7 mM), creatinine (from 77.5 ± 6.3 U/L to 102.2 ± 7.0 μM), and lactate dehydrogenase (from 318.5 ± 26.2 to 380.5 ± 33.2 U/L) confirmed the effect of exercise. WBC count also significantly increased (from 6.6 ± 1.0 to 8.4 ± 1.4 G/L). The level of O-GlcNAc modified proteins in WBCs showed significant elevation after exercise (85 ± 51%, p  < 0.05). Flow cytometry revealed that most of this change could be attributed to lymphocytes and monocytes. Our results indicate that short-term exercise impacts the O-GlcNAc status of WBCs. O-GlcNAc modification could be a natural process by which physical activity modulates the immune system. Further research could elucidate the role of O-GlcNAc during exercise and validate O-GlcNAc as a biomarker for fitness assessment.

Identification and characterization of plant-specific NAC gene family in canola (Brassica napus L.) reveal novel members involved in cell death.

PubMed

Wang, Boya; Guo, Xiaohua; Wang, Chen; Ma, Jieyu; Niu, Fangfang; Zhang, Hanfeng; Yang, Bo; Liang, Wanwan; Han, Feng; Jiang, Yuan-Qing

2015-03-01

NAC transcription factors are plant-specific and play important roles in plant development processes, response to biotic and abiotic cues and hormone signaling. However, to date, little is known about the NAC genes in canola (or oilseed rape, Brassica napus L.). In this study, a total of 60 NAC genes were identified from canola through a systematical analysis and mining of expressed sequence tags. Among these, the cDNA sequences of 41 NAC genes were successfully cloned. The translated protein sequences of canola NAC genes with the NAC genes from representative species were phylogenetically clustered into three major groups and multiple subgroups. The transcriptional activities of these BnaNAC proteins were assayed in yeast. In addition, by quantitative real-time RT-PCR, we further observed that some of these BnaNACs were regulated by different hormone stimuli or abiotic stresses. Interestingly, we successfully identified two novel BnaNACs, BnaNAC19 and BnaNAC82, which could elicit hypersensitive response-like cell death when expressed in Nicotiana benthamiana leaves, which was mediated by accumulation of reactive oxygen species. Overall, our work has laid a solid foundation for further characterization of this important NAC gene family in canola.

PpNAC1, a main regulator of phenylalanine biosynthesis and utilization in maritime pine.

PubMed

Pascual, María Belén; Llebrés, María-Teresa; Craven-Bartle, Blanca; Cañas, Rafael A; Cánovas, Francisco M; Ávila, Concepción

2018-05-01

The transcriptional regulation of phenylalanine metabolism is particularly important in conifers, long-lived species that use large amounts of carbon in wood. Here, we show that the Pinus pinaster transcription factor, PpNAC1, is a main regulator of phenylalanine biosynthesis and utilization. A phylogenetic analysis classified PpNAC1 in the NST proteins group and was selected for functional characterization. PpNAC1 is predominantly expressed in the secondary xylem and compression wood of adult trees. Silencing of PpNAC1 in P. pinaster results in the alteration of stem vascular radial patterning and the down-regulation of several genes associated with cell wall biogenesis and secondary metabolism. Furthermore, transactivation and EMSA analyses showed that PpNAC1 is able to activate its own expression and PpMyb4 promoter, while PpMyb4 is able to activate PpMyb8, a transcriptional regulator of phenylalanine and lignin biosynthesis in maritime pine. Together, these results suggest that PpNAC1 is a functional ortholog of the ArabidopsisSND1 and NST1 genes and support the idea that key regulators governing secondary cell wall formation could be conserved between gymnosperms and angiosperms. Understanding the molecular switches controlling wood formation is of paramount importance for fundamental tree biology and paves the way for applications in conifer biotechnology. © 2017 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

76 FR 13675 - Committee Management; Notice of Establishment

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-14

... NATIONAL SCIENCE FOUNDATION Committee Management; Notice of Establishment The Director of the... consultation with the Committee Management Secretariat, General Services Administration. Name of Committee: U.S..., Arlington, VA 22230. Telephone: 703/292-8030. Dated: March 9, 2011. Susanne Bolton, Committee Management...

77 FR 12331 - Committee Management; Notice of Establishment

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-29

... NATIONAL SCIENCE FOUNDATION Committee Management; Notice of Establishment The Director of the... consultation with the Committee Management Secretariat, General Services Administration. Name of Committee... Bolton, Committee Management Officer. [FR Doc. 2012-4758 Filed 2-28-12; 8:45 am] BILLING CODE 7555-01-P ...

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. Pictured from left-to-right are Congressman Ken Hechler, Democratic representative of West Virginia; Dieter Grau, MSFC; Congressman John W. Davis, Democratic representative of Georgia; Congressman Joe Waggoner, Democratic representative of Louisiana; Congressman Richard L. Roudebush, Republican representative of Indiana; Congressman R. Walter Riehlman, Republican representative of New York; Congressman James G. Fulton, Republican representative of Pennsylvania; Dr. Wernher von Braun, MSFC; and Congressman Olin E. Teague, Democratic representative of Texas.

Members of House Committee on Science and Astronautics Visited MSFC

NASA Technical Reports Server (NTRS)

1962-01-01

The members of the House Committee on Science and Astronautics visited the Marshall Space Flight Center (MSFC) on March 9, 1962 to gather firsthand information of the nation's space exploration program. The congressional group was composed of members of the Subcommittee on Manned Space Flight. They were briefed on MSFC's manned space efforts earlier in the day and then inspected mockups of the Saturn I Workshop and the Apollo Telescope Mount, two projects developed by MSFC for the post-Apollo program. Pictured left-to-right are Dieter Grau, MSFC; Konrad Dannenberg, MSFC; James G. Fulton, Republican representative for Pennsylvania; Joe Waggoner, Democratic representative for Louisiana; and Dr. Wernher von Braun, Director of MSFC.

Overexpression of TaNAC2D Displays Opposite Responses to Abiotic Stresses between Seedling and Mature Stage of Transgenic Arabidopsis

PubMed Central

Huang, Quanjun; Wang, Yan

2016-01-01

Environmental stresses frequently affect plant growth and development, and many genes have been found to be induced by unfavorable environmental conditions. Here, we reported the biological functions of TaNAC2D, a stress-related NAC (NAM, ATAF, and CUC) gene from wheat. TaNAC2D showed transcriptional activator activity in yeast. TaNAC2D-GFP fusion protein was localized in the nucleus of wheat mesophyll protoplasts. TaNAC2D transcript abundance was significantly induced by NaCl, PEG6000, and abscisic acid (ABA) at seedling stage, and repressed by NaCl and PEG6000 at mature plant stage. When TaNAC2D was introduced into Arabidopsis, the 35-day-old soil-grown TaNAC2D-overexpression (TaNAC2D-OX) plants displayed slower stomatal closure, higher water loss rate, and more sensitivity to salt and drought stresses compared with WT plants. In contrast, TaNAC2D-OX seedlings, grown on 1/2 MS medium supplemented with different concentrations of NaCl, Mannitol, and MV, had enhanced tolerances to salt, osmotic and oxidative stresses during seed germination and post-germination periods. The opposite stress-responsive phenotypes of transgenic Arabidopsis were consistent with the expression patterns of TaNAC2D in wheat. Moreover, under high salinity and dehydration conditions, three marker genes, including NCED3, RD29A, and RD29B, were down-regulated in 35-day-old TaNAC2D-OX plants grown in soil and up-regulated in 14-day-old TaNAC2D-OX seedlings grown on 1/2 MS medium. Our results suggest that the change in growth stages and environmental conditions may regulate TaNAC2D’s function. PMID:27933076

miRNA164-directed cleavage of ZmNAC1 confers lateral root development in maize (Zea mays L.).

PubMed

Li, Jing; Guo, Guanghui; Guo, Weiwei; Guo, Ganggang; Tong, Dan; Ni, Zhongfu; Sun, Qixin; Yao, Yingyin

2012-11-21

MicroRNAs are a class of small, non-coding RNAs that regulate gene expression by binding target mRNA, which leads to cleavage or translational inhibition. The NAC proteins, which include NAM, ATAF, and CUC, are a plant-specific transcription factor family with diverse roles in development and stress regulation. It has been reported that miR164 negatively regulates NAC1 expression, which in turn affects lateral root development in Arabidopsis; however, little is known about the involvement of the maize NAC family and miR164 in lateral root development. We collected 175 maize transcripts with NAC domains. Of these, 7 ZmNACs were putative targets for regulation by miR164. We isolated one gene, called TC258020 (designated ZmNAC1) from 2 maize inbred lines, 87-1 and Zong3. ZmNAC1 had a high expression level in roots and showed higher abundance (1.8 fold) in Zong3 relative to 87-1, which had less lateral roots than Zong3. There was a significant correlation between the expression level of ZmNAC1 and the lateral root density in the recombinant inbred line (RIL) population. Transgenic Arabidopsis that overexpressed ZmNAC1 had increased lateral roots in comparison to the wild type. These findings suggest that ZmNAC1 played a significant role in lateral root development. An allelic expression assay showed that trans-regulatory elements were the dominant mediators of ZmNAC1 differential expression in 87-1 and Zong3, and further analysis revealed that miR164 was a trans-element that guided the cleavage of endogenous ZmNAC1 mRNA. Both mature miR164 and miR164 precursors had higher expression in 87-1 than Zong3, which was the opposite of the expression pattern of ZmNAC1. Additionally, the allelic assay showed that the cis-regulatory element most likely affected Zm-miR164b's expression pattern. A β-glucuronidase (GUS) assay showed that the Zm-miR164b promoter had higher GUS activity in 87-1 than in Zong3. In addition, we detected miR164b expression in the RIL population, and the

Epigenetic activation of MGAT3 and corresponding bisecting GlcNAc shortens the survival of cancer patients.

PubMed

Kohler, Reto S; Anugraham, Merrina; López, Mónica Núñez; Xiao, Christina; Schoetzau, Andreas; Hettich, Timm; Schlotterbeck, Goetz; Fedier, André; Jacob, Francis; Heinzelmann-Schwarz, Viola

2016-08-09

Bisecting GlcNAc on N-glycoproteins is described in E-cadherin-, EGF-, Wnt- and integrin- cancer-associated signaling pathways. However, the mechanisms regulating bisecting GlcNAc expression are not clear. Bisecting GlcNAc is attached to N-glycans through beta 1-4 N-acetylglucosaminyl transferase III (MGAT3), which is encoded by two exons flanked by high-density CpG islands. Despite a recently described correlation of MGAT3 and bisecting GlcNAc in ovarian cancer cells, it remains unknown whether DNA methylation is causative for the presence of bisecting GlcNAc. Here, we narrow down the regulatory genomic region and show that reconstitution of MGAT3 expression with 5-Aza coincides with reduced DNA methylation at the MGAT3 transcription start site. The presence of bisecting GlcNAc on released N-glycans was detected by mass spectrometry (LC-ESI-qTOF-MS/MS) in serous ovarian cancer cells upon DNA methyltransferase inhibition. The regulatory impact of DNA methylation on MGAT3 was further evaluated in 18 TCGA cancer types (n = 6118 samples) and the results indicate an improved overall survival in patients with reduced MGAT3 expression, thereby identifying long-term survivors of high-grade serous ovarian cancers (HGSOC). Epigenetic activation of MGAT3 was also confirmed in basal-like breast cancers sharing similar molecular and genetic features with HGSOC. These results provide novel insights into the epigenetic regulation of MGAT3/bisecting GlcNAc and demonstrate the importance of N-glycosylation in cancer progression.

Electronic Information Delivery Systems. Proceedings of the Library of Congress Network Advisory Committee Meeting (Washington, D.C., April 18-20, 1984). Network Planning Paper Number 9.

ERIC Educational Resources Information Center

Library of Congress, Washington, DC.

The Program Session of the April 1984 meeting of the Library of Congress Network Advisory Committee (NAC) was devoted to discussion of electronic information delivery systems. Recent developments in six areas were covered: (1) electronic manuscript generation and transmission; (2) online full-text searching and retrieval; (3) online database…

O-GlcNAc modification of radial glial vimentin filaments in the developing chick brain.

PubMed

Farach, Andrew M; Galileo, Deni S

2008-12-01

We examined the post-translational modification of intracellular proteins by beta-O-linked N-acetylglucosamine (O-GlcNAc) with regard to neurofilament phosphorylation in the developing chick optic tectum. A regulated developmental pattern of O-GlcNAcylation was discovered in the developing brain. Most notably, discernible staining occurs along radial glial filaments but not along neuronal filaments in vivo. Immunohistochemical analyses in sections of progressive stages of development suggest upregulation of O-GlcNAc in the ependyma, tectofugal neuron bodies, and radial glial processes, but not in axons. In contrast, double-label immunostaining of monolayer cultures made from dissociated embryonic day (E) 7 optic tecta revealed O-GlcNAcylation of most axons. Labeling of brain sections together with Western blot analyses showed O-GlcNAc modification of a few discrete proteins throughout development, and suggested vimentin as the protein in radial glia. Immunoprecipitation of vimentin from E9 whole brain lysates confirmed O-GlcNAcylation of vimentin in development. These results indicate a regulated pattern of O-GlcNAc modification of vimentin filaments, which in turn suggests a role for O-GlcNAc-modified intermediate filaments in radial glia, but not in neurons during brain development. The control mechanisms that regulate this pattern in vivo, however, are disrupted when cells are placed in vitro.

The Commons Science and Technology Committee inquiry into hybrid embryo research 2007: credible, reliable and objective?

PubMed

Gately, Pauline

2011-01-01

In 2006 the Government issued a white paper in which it proposed a ban on human-animal embryo research pending greater clarity on its potential. The Commons Select Committee on Science and Technology initiated an Inquiry and concluded that such research was necessary and should be permitted immediately. The Government agreed and this is reflected in revised legislation. The Government has issued guidelines on the gathering and use of scientific advice and evidence, designed to ensure that these are "credible, reliable and objective." This article tests the Committee's approach in the light of its remit and these, and other, relevant guidelines and concludes that it failed to meet these standards. Rather it effectively ceded to an interest group the regulation of its own activities. The article ends by suggesting alterations to the Committee's remit and composition designed to ensure that the public interest is better protected in future.

Tobacco Advertising and Children. Hearing before the Committee on Commerce, Science, and Transportation. United States Senate, 105th Congress, 1st Session.

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. Senate Committee on Commerce, Science, and Transportation.

This booklet provides a transcript of the September 16, 1997 hearing before the Committee on Commerce, Science, and Transportation of the U.S. Senate. The hearing concerns tobacco advertising and children. The statements delivered before the committee as well as the prepared statements of several senators are included. These senators are: John…

Differential Expression Analysis of a Subset of Drought-Responsive GmNAC Genes in Two Soybean Cultivars Differing in Drought Tolerance

PubMed Central

Thao, Nguyen Phuong; Thu, Nguyen Binh Anh; Hoang, Xuan Lan Thi; Van Ha, Chien; Tran, Lam-Son Phan

2013-01-01

The plant-specific NAC transcription factors play important roles in plant response to drought stress. Here, we have compared the expression levels of a subset of GmNAC genes in drought-tolerant DT51 and drought-sensitive MTD720 under both normal and drought stress conditions aimed at identifying correlation between GmNAC expression levels and drought tolerance degree, as well as potential GmNAC candidates for genetic engineering. The expression of 23 selected dehydration-responsive GmNACs was assessed in both stressed and unstressed root tissues of DT51 and MTD720 using real-time quantitative PCR. The results indicated that expression of GmNACs was genotype-dependent. Seven and 13 of 23 tested GmNACs showed higher expression levels in roots of DT51 in comparison with MTD720 under normal and drought stress conditions, respectively, whereas none of them displayed lower transcript levels under any conditions. This finding suggests that the higher drought tolerance of DT51 might be positively correlated with the higher induction of the GmNAC genes during water deficit. The drought-inducible GmNAC011 needs to be mentioned as its transcript accumulation was more than 76-fold higher in drought-stressed DT51 roots relative to MTD720 roots. Additionally, among the GmNAC genes examined, GmNAC085, 092, 095, 101 and 109 were not only drought-inducible but also more highly up-regulated in DT51 roots than in that of MTD720 under both treatment conditions. These data together suggest that GmNAC011, 085, 092, 095, 101 and 109 might be promising candidates for improvement of drought tolerance in soybean by biotechnological approaches. PMID:24322442

Genome-wide characterization and expression profiling of NAC transcription factor genes under abiotic stresses in radish (Raphanus sativus L.)

PubMed Central

Muleke, Everlyne M’mbone; Jabir, Bashir Mohammed; Xie, Yang; Zhu, Xianwen; Cheng, Wanwan

2017-01-01

NAC (NAM, no apical meristem; ATAF, Arabidopsis transcription activation factor and CUC, cup-shaped cotyledon) proteins are among the largest transcription factor (TF) families playing fundamental biological processes, including cell expansion and differentiation, and hormone signaling in response to biotic and abiotic stresses. In this study, 172 RsNACs comprising 17 membrane-bound members were identified from the whole radish genome. In total, 98 RsNAC genes were non-uniformly distributed across the nine radish chromosomes. In silico analysis revealed that expression patterns of several NAC genes were tissue-specific such as a preferential expression in roots and leaves. In addition, 21 representative NAC genes were selected to investigate their responses to heavy metals (HMs), salt, heat, drought and abscisic acid (ABA) stresses using real-time polymerase chain reaction (RT-qPCR). As a result, differential expressions among these genes were identified where RsNAC023 and RsNAC080 genes responded positively to all stresses except ABA, while RsNAC145 responded more actively to salt, heat and drought stresses compared with other genes. The results provides more valuable information and robust candidate genes for future functional analysis for improving abiotic stress tolerances in radish. PMID:29259849

78 FR 56944 - Advisory Committee for Geosciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-16

... atmospheric, geo-space, earth, ocean and polar sciences. Agenda October 9, 2013 Meeting with the Acting... NATIONAL SCIENCE FOUNDATION Advisory Committee for Geosciences; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as amended), the National Science Foundation...

Comparative Genomics of NAC Transcriptional Factors in Angiosperms: Implications for the Adaptation and Diversification of Flowering Plants

PubMed Central

Pereira-Santana, Alejandro; Alcaraz, Luis David; Castaño, Enrique; Sanchez-Calderon, Lenin; Sanchez-Teyer, Felipe; Rodriguez-Zapata, Luis

2015-01-01

NAC proteins constitute one of the largest groups of plant-specific transcription factors and are known to play essential roles in various developmental processes. They are also important in plant responses to stresses such as drought, soil salinity, cold, and heat, which adversely affect growth. The current knowledge regarding the distribution of NAC proteins in plant lineages comes from relatively small samplings from the available data. In the present study, we broadened the number of plant species containing the NAC family origin and evolution to shed new light on the evolutionary history of this family in angiosperms. A comparative genome analysis was performed on 24 land plant species, and NAC ortholog groups were identified by means of bidirectional BLAST hits. Large NAC gene families are found in those species that have experienced more whole-genome duplication events, pointing to an expansion of the NAC family with divergent functions in flowering plants. A total of 3,187 NAC transcription factors that clustered into six major groups were used in the phylogenetic analysis. Many orthologous groups were found in the monocot and eudicot lineages, but only five orthologous groups were found between P. patens and each representative taxa of flowering plants. These groups were called basal orthologous groups and likely expanded into more recent taxa to cope with their environmental needs. This analysis on the angiosperm NAC family represents an effort to grasp the evolutionary and functional diversity within this gene family while providing a basis for further functional research on vascular plant gene families. PMID:26569117

Comparative Genomics of NAC Transcriptional Factors in Angiosperms: Implications for the Adaptation and Diversification of Flowering Plants.

PubMed

Pereira-Santana, Alejandro; Alcaraz, Luis David; Castaño, Enrique; Sanchez-Calderon, Lenin; Sanchez-Teyer, Felipe; Rodriguez-Zapata, Luis

2015-01-01

NAC proteins constitute one of the largest groups of plant-specific transcription factors and are known to play essential roles in various developmental processes. They are also important in plant responses to stresses such as drought, soil salinity, cold, and heat, which adversely affect growth. The current knowledge regarding the distribution of NAC proteins in plant lineages comes from relatively small samplings from the available data. In the present study, we broadened the number of plant species containing the NAC family origin and evolution to shed new light on the evolutionary history of this family in angiosperms. A comparative genome analysis was performed on 24 land plant species, and NAC ortholog groups were identified by means of bidirectional BLAST hits. Large NAC gene families are found in those species that have experienced more whole-genome duplication events, pointing to an expansion of the NAC family with divergent functions in flowering plants. A total of 3,187 NAC transcription factors that clustered into six major groups were used in the phylogenetic analysis. Many orthologous groups were found in the monocot and eudicot lineages, but only five orthologous groups were found between P. patens and each representative taxa of flowering plants. These groups were called basal orthologous groups and likely expanded into more recent taxa to cope with their environmental needs. This analysis on the angiosperm NAC family represents an effort to grasp the evolutionary and functional diversity within this gene family while providing a basis for further functional research on vascular plant gene families.

O-GlcNAc transferase enables AgRP neurons to suppress browning of white fat

PubMed Central

Ruan, Hai-Bin; Dietrich, Marcelo O.; Liu, Zhong-Wu; Zimmer, Marcelo R.; Li, Min-Dian; Singh, Jay Prakash; Zhang, Kaisi; Yin, Ruonan; Wu, Jing; Horvath, Tamas L.; Yang, Xiaoyong

2014-01-01

SUMMARY Induction of beige cells causes the browning of white fat and improves energy metabolism. However, the central mechanism that controls adipose tissue browning and its physiological relevance are largely unknown. Here we demonstrate that fasting and chemical-genetic activation of orexigenic AgRP neurons in the hypothalamus suppress the browning of white fat. O-linked β-N-acetylglucosamine (O-GlcNAc) modification of cytoplasmic and nuclear proteins regulates fundamental cellular processes. The levels of O-GlcNAc transferase (OGT) and O-GlcNAc modification are enriched in AgRP neurons and are elevated by fasting. Genetic ablation of OGT in AgRP neurons inhibits neuronal excitability through the voltage-dependent potassium channel, promotes white adipose tissue browning, and protects mice against diet-induced obesity and insulin resistance. These data reveal adipose tissue browning as a highly dynamic physiological process under central control, in which O-GlcNAc signaling in AgRP neurons is essential for suppressing thermogenesis to conserve energy in response to fasting. PMID:25303527

The NAC transcription factor family in maritime pine (Pinus Pinaster): molecular regulation of two genes involved in stress responses.

PubMed

Pascual, Ma Belén; Cánovas, Francisco M; Ávila, Concepción

2015-10-24

NAC transcription factors comprise a large plant-specific gene family involved in the regulation of diverse biological processes. Despite the growing number of studies on NAC transcription factors in various species, little information is available about this family in conifers. The goal of this study was to identify the NAC transcription family in maritime pine (Pinus pinaster), to characterize ATAF-like genes in response to various stresses and to study their molecular regulation. We have isolated two maritime pine NAC genes and using a transient expression assay in N. benthamiana leaves estudied the promoter jasmonate response. In this study, we identified 37 NAC genes from maritime pine and classified them into six main subfamilies. The largest group includes 12 sequences corresponding to stress-related genes. Two of these NAC genes, PpNAC2 and PpNAC3, were isolated and their expression profiles were examined at various developmental stages and in response to various types of stress. The expression of both genes was strongly induced by methyl jasmonate (MeJA), mechanical wounding, and high salinity. The promoter regions of these genes were shown to contain cis-elements involved in the stress response and plant hormonal regulation, including E-boxes, which are commonly found in the promoters of genes that respond to jasmonate, and binding sites for bHLH proteins. Using a transient expression assay in N. benthamiana leaves, we found that the promoter of PpNAC3 was rapidly induced upon MeJA treatment, while this response disappeared in plants in which the transcription factor NbbHLH2 was silenced. Our results suggest that PpNAC2 and PpNAC3 encode stress-responsive NAC transcription factors involved in the jasmonate response in pine. Furthermore, these data also suggest that the jasmonate signaling pathway is conserved between angiosperms and gymnosperms. These findings may be useful for engineering stress tolerance in pine via biotechnological approaches.

Post-Secondary Transfers. ACCC Submission to the Standing Senate Committee on Social Affairs, Science and Technology

ERIC Educational Resources Information Center

Association of Canadian Community Colleges, 2010

2010-01-01

This report provides the recommendations made by the Association of Canadian Community Colleges (ACCC) to the Standing Senate Committee on Social Affairs, Science and Technology based from its examination on the accessibility of post-secondary education in Canada. Aligned with the needs of employers, and operating on the leading edge of advanced…

Personality and culture, the Social Science Research Council, and liberal social engineering: the Advisory Committee on Personality and Culture, 1930-1934.

PubMed

Bryson, Dennis

2009-01-01

The field of personality and culture was given a significant impetus during the 1930s with the establishment of the Advisory Committee on Personality and Culture (1930-1934) by the Social Science Research Council. This committee provided an early formulation of personality and culture that emphasized the interdisciplinary focus on the processes of personality formation within small-scale social settings. The committee's formulation also coupled personality and culture with a liberal social engineering approach geared toward cultural reconstruction. Major social scientists and clinicians were involved in the activities of the committee, including Edward Sapir, W. I. Thomas, E. W. Burgess, E. A. Bott, Robert S. Woodworth, Harry Stack Sullivan, C. M. Hincks, and Adolf Meyer.

76 FR 12136 - Advisory Committee for Geosciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-04

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Geosciences; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as amended), the National Science Foundation announces the following meeting: Name: Advisory Committee for Geosciences (1755). Dates: April 13, 2011; 8:30 a.m.- 5 p.m., April 14, 2011; 8:30 a.m...

78 FR 38736 - Committee Management; Notice of Establishment

Federal Register 2010, 2011, 2012, 2013, 2014

2013-06-27

... NATIONAL SCIENCE FOUNDATION Committee Management; Notice of Establishment The Director of the... determination follows consultation with the Committee Management Secretariat, General Services Administration.../ 292-8040. Dated: June 24, 2013. Susanne Bolton, Committee Management Officer. [FR Doc. 2013-15384...

Isolation and expression profiling of GhNAC transcription factor genes in cotton (Gossypium hirsutum L.) during leaf senescence and in response to stresses.

PubMed

Shah, Syed Tariq; Pang, Chaoyou; Fan, Shuli; Song, Meizhen; Arain, Saima; Yu, Shuxun

2013-12-01

NAC (NAM, ATAF, and CUC) is a plant-specific transcription factor family with diverse roles in plant development and stress regulation. In this report, stress-responsive NAC genes (GhNAC8-GhNAC17) isolated from cotton (Gossypium hirsutum L.) were characterised in the context of leaf senescence and stress tolerance. The characterisation of NAC genes during leaf senescence has not yet been reported for cotton. Based on the sequence characterisation, these GhNACs could be classified into three groups belonging to three known NAC sub-families. Their predicted amino acid sequences exhibited similarities to NAC genes from other plant species. Senescent leaves were the sites of maximum expression for all GhNAC genes except GhNAC10 and GhNAC13, which showed maximum expression in fibres, collected from 25 days post anthesis (DPA) plants. The ten GhNAC genes displayed differential expression patterns and levels during natural and induced leaf senescence. Quantitative RT-PCR and promoter analyses suggest that these genes are induced by ABA, ethylene, drought, salinity, cold, heat, and other hormonal treatments. These results support a role for cotton GhNAC genes in transcriptional regulation of leaf senescence, stress tolerance and other developmental stages of cotton. © 2013.

The ubiquitin ligase SEVEN IN ABSENTIA (SINA) ubiquitinates a defense-related NAC transcription factor and is involved in defense signaling.

PubMed

Miao, Min; Niu, Xiangli; Kud, Joanna; Du, Xinran; Avila, Julian; Devarenne, Timothy P; Kuhl, Joseph C; Liu, Yongsheng; Xiao, Fangming

2016-07-01

We recently identified a defense-related tomato (Solanum lycopersicum) NAC (NAM, ATAF1,2, CUC2) transcription factor, NAC1, that is subjected to ubiquitin-proteasome system-dependent degradation in plant cells. In this study, we identified a tomato ubiquitin ligase (termed SEVEN IN ABSENTIA3; SINA3) that ubiquitinates NAC1, promoting its degradation. We conducted coimmunoprecipitation and bimolecular fluorescence complementation to determine that SINA3 specifically interacts with the NAC1 transcription factor in the nucleus. Moreover, we found that SINA3 ubiquitinates NAC1 in vitro and promotes NAC1 degradation via polyubiquitination in vivo, indicating that SINA3 is a ubiquitin ligase that ubiquitinates NAC1, promoting its degradation. Our real-time PCR analysis indicated that, in contrast to our previous finding that NAC1 mRNA abundance increases upon Pseudomonas infection, the SINA3 mRNA abundance decreases in response to Pseudomonas infection. Moreover, using Agrobacterium-mediated transient expression, we found that overexpression of SINA3 interferes with the hypersensitive response cell death triggered by multiple plant resistance proteins. These results suggest that SINA3 ubiquitinates a defense-related NAC transcription factor for degradation and plays a negative role in defense signaling. © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust.

76 FR 72954 - Request for Nominations for Voting Members on Public Advisory Committee, Science Board to the...

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2011-11-28

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0002] Request for Nominations for Voting Members on Public Advisory Committee, Science Board to the Food and Drug Administration AGENCY: Food and Drug Administration, HHS. ACTION: Notice. The Food and Drug...

75 FR 55360 - Advisory Committee for Geosciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-10

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Geosciences; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as amended), the National Science Foundation...-October 7, 2010; 8:30 a.m.-1:30 p.m. Place: Stafford I, Room 1235, National Science Foundation, 4201...

Synthesis of the 3-sulfates of N-acetylcysteine conjugated bile acids (BA-NACs) and their transient formation from BA-NACs and subsequent hydrolysis by a rat liver cytosolic fraction as shown by liquid chromatography/electrospray ionization-mass spectrometry.

PubMed

Mitamura, Kuniko; Sakai, Toshihiro; Nakai, Risa; Wakamiya, Tateaki; Iida, Takashi; Hofmann, Alan F; Ikegawa, Shigeo

2011-06-01

Previous work from this laboratory has reported the chemical synthesis of N-acetylcysteine (NAC) conjugates of natural bile acids (BAs) and shown that such novel conjugates can be formed in vivo in rats to which NAC has been administered. The subsequent fate of such novel conjugates is not known. One possible biotransformation is sulfation, a major pathway for BAs N-acylamidates in patients with cholestatic liver disease. Here, we report the chemical synthesis of the 3-sulfates of the S-acyl NAC conjugates of five natural BAs (cholic, chenodeoxycholic, deoxycholic, ursodeoxycholic, and lithocholic). We also measured the sulfation of N-acetylcysteine-natural bile acid (BA-NAC) conjugates when they were incubated with a rat liver cytosolic fraction. The chemical structures of the BA-NAC 3-sulfates were confirmed by proton nuclear magnetic resonance, as well as by means of electrospray ionization-linear ion trap mass spectrometry with negative-ion detection. Upon collision-induced dissociation of singly and doubly charged deprotonated molecules, structurally informative product ions were observed. Using a triple-stage quadrupole instrument, selected reaction monitoring analyses by monitoring characteristic transition ions allowed the achievement of a highly sensitive and specific assay. When BA-NACs were incubated with a rat liver cytosolic fraction to which 3'-phosphoadenosine 5'-phosphosulfate was added, sulfation occurred, but the dominant reaction was hydrolysis of the S-acyl linkage to form the unconjugated BAs. Subsequent sulfation occurred at C-3 on the unconjugated BAs that had been formed from the BA-NACs. Such sulfation was proportional to the hydrophobicity of the unconjugated bile acid. Thus, NAC conjugates of BAs as well as their C-3 sulfates if formed in vivo are rapidly hydrolyzed by cytosolic enzymes.

76 FR 9765 - Advanced Scientific Computing Advisory Committee

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-22

... DEPARTMENT OF ENERGY Advanced Scientific Computing Advisory Committee AGENCY: Office of Science... Advanced Scientific Computing Advisory Committee (ASCAC). The Federal Advisory Committee Act (Pub. L. 92... INFORMATION CONTACT: Melea Baker, Office of Advanced Scientific Computing Research, SC-21/Germantown Building...

F44. AN ADD-ON TRIAL WITH N-ACETYL-CYSTEINE (NAC) IN EARLY PSYCHOSIS PATIENTS: TOWARDS BIOMARKER GUIDED TREATMENT

PubMed Central

Conus, Philippe; Fournier, Margot; Xin, Lijing; Cleusix, Martine; Baumann, Philipp S; Ferrari, Carina; Cousins, Ann; Alameda, Luis; Gholam-Razaee, Mehdi; Golay, Philippe; Jenni, Raoul; Woo, Tsung-Ung Wilson; Keshavan, Matcheri; Eap, Chin B; Wojcik, Joanne; Cuenod, Michel; Buclin, Thierry; Gruetter, Rolf; Seidman, Larry; Do, Kim

2018-01-01

Abstract Background Oxidative stress, coupled with dysregulation of inflammation, NMDAR and dopamine, is involved in schizophrenia (SZ) pathophysiology. Earlier add-on clinical trials showed in chronic SZ patients that NAC, a precursor of glutathione (GSH), an important cerebral antioxidant, improved negative symptoms, mismatch negativity and local synchronization. We hypothesized that NAC at an earlier stage of illness would have a greater impact. Methods Early psychosis patients (EP, less than 5 years of illness, N=63; NAC=32, placebo=31) were supplemented with NAC (2.7g/day, 6 months) in a double-blind randomized placebo-controlled trial. Outcome measures: PANSS and neurocognition (MATRICS Consensus Cognitive Battery; n=36); quantification of medial prefronfal cortex glutathione (GSHmPFC) by 1H-magnetic-resonance-spectroscopy, of white matter diffusion properties estimated by generalized fractional anisotropy (gFA) computed from diffusion spectrum imaging (DSI), of blood cells GSH (GSHBC) and GSH peroxidase activity (GPxBC) at start and end of trial Results While PANSS negative and positive were not affected by NAC, NAC improved Processing Speed (NAC > Placebo; F(1, 30)=5.849, p=.022), favoring 2 of 3 processing speed tasks (Trail Making A, F(1, 30)=4.279, p=.048 & Verbal Fluency, F(1, 30)=5.749, p=.023). GSHmPFC (+23%, p=0.005) and GSHBC (+19%, p=0.05) were increased following NAC treatment. In patients with high-baseline GPxBC (>22.3U/gHb), subgroup explorations revealed an improvement of PANSS positive compared to placebo (p=0.02). The change of PANSS positive correlated negatively with that of GPxBC activity, showing that the improvement paralleled the restoration of redox status. NAC group showed 11% increase in fornix white matter integrity as measured by gFA, correlating with an increase in GSHmPFC over the 6-months period. Discussion This is the first clinical trial assessing the impact of NAC treatment in a sample of EP and the potential predictive role

78 FR 24239 - Advisory Committee for Cyberinfrastructure; Notice of Meeting

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2013-04-24

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Cyberinfrastructure; Notice of Meeting In... Foundation announces the following meeting: Name: Advisory Committee for Cyberinfrastructure (25150). Date... Person: Marc Rigas, Advanced Cyberinfrastructure (CISE/ ACI), National Science Foundation, 4201 Wilson...

76 FR 14436 - Advisory Committee for Cyberinfrastructure; Notice of Meeting

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2011-03-16

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Cyberinfrastructure; Notice of Meeting In... Foundation announces the following meeting: Name: Advisory Committee for Cyberinfrastructure (25150). Date... of the Director, Office of Cyberinfrastructure (OD/OCI), National Science Foundation, 4201 Wilson...

76 FR 69769 - Advisory Committee for Cyberinfrastructure; Notice of Meeting

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2011-11-09

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Cyberinfrastructure; Notice of Meeting In... Foundation announces the following meeting: Name: Advisory Committee for Cyberinfrastructure (25150). Date... Director, Office of Cyberinfrastructure (OD/OCI), National Science Foundation, 4201 Wilson Blvd., Suite...

77 FR 31044 - Advisory Committee for Cyberinfrastructure; Notice of Meeting

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2012-05-24

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Cyberinfrastructure; Notice of Meeting In... Foundation announces the following meeting: Name: Advisory Committee for Cyberinfrastructure (25150). Date..., Office of Cyberinfrastructure (OD/OCI), National Science Foundation, 4201 Wilson Blvd., Suite 1145...

75 FR 13313 - Advisory Committee for Geosciences; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-19

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Geosciences; Notice of Meeting In accordance with the Federal Advisory Committee Act (Pub. L. 92- 463, as amended), the National Science Foundation...:30 a.m.-5 p.m. April 15, 2010 8:30, a.m.-1:30 p.m. Place: Stafford I, Room 1235, National Science...

77 FR 58871 - Advisory Committee for Cyberinfrastructure; Notice of Meeting

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-24

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Cyberinfrastructure; Notice of Meeting In... Foundation announces the following meeting: Name: Advisory Committee for Cyberinfrastructure (25150). Date..., Office of the Director, Office of Cyberinfrastructure (OD/OCI), National Science Foundation, 4201 Wilson...

Academic health sciences librarians' contributions to institutional animal care and use committees.

PubMed

Steelman, Susan C; Thomas, Sheila L

2014-07-01

The study gathered data about librarians' membership in institutional animal care and use committees (IACUCs) and their professional activities supporting animal researchers. Libraries affiliated with medical schools that were members of the Association of American Medical Colleges were surveyed. A survey was distributed via library directors' email discussion lists and direct email messages. Sixty surveys were completed: 35 (58%) reported that librarians performed database searches for researchers, and 22 (37%) reported that a librarian currently serves on the IACUC. The survey suggests that academic health sciences librarians provide valuable, yet underutilized, services to support animal research investigators.

Defining the carbohydrate specificities of Abrus precatorius agglutinin as T (Gal beta 1----3GalNAc) greater than I/II (Gal beta 1----3/4GlcNAc).

PubMed

Wu, A M; Lin, S R; Chin, L K; Chow, L P; Lin, J Y

1992-09-25

The combining site of the nontoxic carbohydrate binding protein (Abrus precatorius agglutinin, APA) purified from the needs of Abrus precatorius (Jequirity bean), was studied by quantitative precipitin and precipitin-inhibition assays. Of 26 glycoproteins and polysaccharides tested, all, except sialic acid-containing glycoproteins and desialized ovine salivary glycoproteins, reacted strongly with the lectin, and precipitated over 70% of the lectin added, indicating that APA has a broad range of affinity and recognizes (internal) Gal beta 1----sequences of carbohydrate chains. The strong reaction with desialized porcine and rat salivary glycoproteins as well as pneumococcus type XIV polysaccharide suggests that APA has affinity for one or more of the following carbohydrate sequences: Thomsen-Friedenreich (T, Gal beta 1----3GalNAc), blood group precursor type I and/or type II (Gal beta 1----3/4GlcNAc) disaccharide determinants of complex carbohydrates. Among the oligosaccharides tested, the T structure was the best inhibitor; it was 2.4 and 3.2 times more active than type II and type I sequences, respectively. The blood group I Ma-active trisaccharide, Gal beta 1----4GlcNAc beta 1----6Gal, was about as active as the corresponding disaccharide (II). From the above results, we conclude that the size of the combining site of the A. precatorius agglutinin is probably as large as a disaccharide and most strongly complementary to the Gal beta 1----3GalNAc (T determinant) sequence. The carbohydrate specificities of this lectin will be further investigated once the related oligosaccharide structures become available.

Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli.

PubMed

Lauber, Jennifer; Handrick, René; Leptihn, Sebastian; Dürre, Peter; Gaisser, Sabine

2015-01-13

Recombinant protein-based therapeutics have become indispensable for the treatment of many diseases. They are produced using well-established expression systems based on bacteria, yeast, insect and mammalian cells. The majority of therapeutic proteins are glycoproteins and therefore the post-translational attachment of sugar residues is required. The development of an engineered Escherichia coli-based expression system for production of human glycoproteins could potentially lead to increased yields, as well as significant decreases in processing time and costs. This work describes the expression of functional human-derived glycosyltransferase UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAcT2) in a recombinant E. coli strain. For expression, a codon-optimised gene encoding amino acids 52-571 of GalNAcT2 lacking the transmembrane N-terminal domain was inserted into a pET-23 derived vector encoding a polyhistidine-tag which was translationally fused to the N-terminus of the glycosyltransferase (HisDapGalNAcT2). The glycosyltransferase was produced in E. coli using a recently published expression system. Soluble HisDapGalNAcT2 produced in SHuffle® T7 host cells was purified using nickel affinity chromatography and was subsequently analysed by size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) and circular dichroism spectroscopy to determine molecular mass, folding state and thermal transitions of the protein. The activity of purified HisDapGalNAcT2 was monitored using a colorimetric assay based on the release of phosphate during transfer of glycosyl residues to a model acceptor peptide or, alternatively, to the granulocyte-colony stimulating growth factor (G-CSF). Modifications were assessed by Matrix Assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry analysis (MALDI-TOF-MS) and Electrospray Mass Spectrometry analysis (ESI-MS). The results clearly indicate the glycosylation of the acceptor peptide and

75 FR 20627 - Advisory Committee for Cyberinfrastructure; Notice of Meeting

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2010-04-20

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Cyberinfrastructure; Notice of Meeting In... Foundation announces the following meeting: Name: Advisory Committee for Cyberinfrastructure (25150) Date and... Oberright, Office of the Director, Office of Cyberinfrastructure (OD/OCI), National Science Foundation, 4201...

78 FR 20957 - Business and Operations Advisory Committee; Notice of Meeting

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2013-04-08

... NATIONAL SCIENCE FOUNDATION Business and Operations Advisory Committee; Notice of Meeting In accordance with Federal Advisory Committee Act (Pub. L. 92-463, as amended), the National Science Foundation announces the following meeting: Name: Business and Operations Advisory Committee (9556). Date/Time: April 30, 2013; 1:00 p.m. to 5:30 p.m. (EST)...

76 FR 64388 - Advisory Committee for Polar Programs; Notice of Meeting

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2011-10-18

... NATIONAL SCIENCE FOUNDATION Advisory Committee for Polar Programs; Notice of Meeting In accordance with Federal Advisory Committee Act (Pub. L. 92-463, as amended), the National Science Foundation announces the following meeting: Name: Advisory Committee for Polar Programs (1130). Date/Time: November 14, 2011, 8:30 a.m. to 5:30 p.m.; November 15...

76 FR 44963 - Committee Management; Renewals

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2011-07-27

... NATIONAL SCIENCE FOUNDATION Committee Management; Renewals The NSF management officials having... USC 1861 et seq. This determination follows consultation with the Committee Management Secretariat... Management Officer. [FR Doc. 2011-18934 Filed 7-26-11; 8:45 am] BILLING CODE 7555-01-P ...

Global Expressions Landscape of NAC Transcription Factor Family and Their Responses to Abiotic Stresses in Citrullus lanatus

PubMed Central

Lv, Xiaolong; Lan, Shanrong; Guy, Kateta Malangisha; Yang, Jinghua; Zhang, Mingfang; Hu, Zhongyuan

2016-01-01

Watermelon (Citrullus lanatus) is one xerophyte that has relative higher tolerance to drought and salt stresses as well as more sensitivity to cold stress, compared with most model plants. These characteristics facilitate it a potential model crop for researches on salt, drought or cold tolerance. In this study, a genome-wide comprehensive analysis of the ClNAC transcription factor (TF) family was carried out for the first time, to investigate their transcriptional profiles and potential functions in response to these abiotic stresses. The expression profiling analysis reveals that several NAC TFs are highly responsive to abiotic stresses and development, for instance, subfamily IV NACs may play roles in maintaining water status under drought or salt conditions, as well as water and metabolites conduction and translocation toward fruit. In contrast, rapid and negative responses of most of the ClNACs to low-temperature adversity may be related to the sensitivity to cold stress. Crosstalks among these abiotic stresses and hormone (abscisic acid and jasmonic acid) pathways were also discussed based on the expression of ClNAC genes. Our results will provide useful insights for the functional mining of NAC family in watermelon, as well as into the mechanisms underlying abiotic tolerance in other cash crops. PMID:27491393

Global Expressions Landscape of NAC Transcription Factor Family and Their Responses to Abiotic Stresses in Citrullus lanatus.

PubMed

Lv, Xiaolong; Lan, Shanrong; Guy, Kateta Malangisha; Yang, Jinghua; Zhang, Mingfang; Hu, Zhongyuan

2016-08-05

Watermelon (Citrullus lanatus) is one xerophyte that has relative higher tolerance to drought and salt stresses as well as more sensitivity to cold stress, compared with most model plants. These characteristics facilitate it a potential model crop for researches on salt, drought or cold tolerance. In this study, a genome-wide comprehensive analysis of the ClNAC transcription factor (TF) family was carried out for the first time, to investigate their transcriptional profiles and potential functions in response to these abiotic stresses. The expression profiling analysis reveals that several NAC TFs are highly responsive to abiotic stresses and development, for instance, subfamily IV NACs may play roles in maintaining water status under drought or salt conditions, as well as water and metabolites conduction and translocation toward fruit. In contrast, rapid and negative responses of most of the ClNACs to low-temperature adversity may be related to the sensitivity to cold stress. Crosstalks among these abiotic stresses and hormone (abscisic acid and jasmonic acid) pathways were also discussed based on the expression of ClNAC genes. Our results will provide useful insights for the functional mining of NAC family in watermelon, as well as into the mechanisms underlying abiotic tolerance in other cash crops.