Volunteer Scientist-in-the-Classroom Partnership in Metropolitan Nashville Public Schools

ERIC Educational Resources Information Center

Tuschl, Jeannie

2008-01-01

In 1897, long before the entertainment industry made Nashville famous with country music and the Grand Ole Opry, the city was known as the "Athens of the South," with numerous institutions of higher learning. A century later, four of Nashville's oldest universities have united to promote science education in Metropolitan Nashville Public Schools…

Nashville ITS strategic plan : an intelligent transportation system for the Nashville region : executive summary

DOT National Transportation Integrated Search

1997-06-25

In October of 1995, the Nashville Area Metropolitan Planning Organization (MPO), in cooperation with the Tennessee Department of Transportation (TDOT) and the Federal Highway Administration (FHWA) contracted with Kimley-Horn and Associates to prepare...

A History of Special Education in Nashville, Tennessee, 1940-1990. Final Report.

ERIC Educational Resources Information Center

Dorn, Sherman

This review of the history of special education in Nashville, Tennessee, from 1940 to 1990 focuses on two issues: first, how advocacy for children with disabilities and research changed the practice of special education after World War II in Nashville; and second, how the internal dynamics of the schools in Nashville shaped special education…

75 FR 31836 - Tennessee Division: Notice To Rescind a Notice of Intent (NOI) to Prepare an Environmental Impact...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-04

...'Neill, Planning and Program Management Team leader, FHWA-Tennessee Division Office, 404 BNA Drive, Suite 508, Nashville, TN 37217. SUPPLEMENTARY INFORMATION: The FHWA, in cooperation with the Tennessee... this proposed program.) Charles J. O'Neill, Planning and Program Management Team Leader, Nashville, TN...

75 FR 57549 - Notice To Rescind a Notice of Intent To Prepare a Supplemental Environmental Impact Statement...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-21

... Management Team Leader, Federal Highway Administration-- Tennessee Division Office, 404 BNA Drive, Suite 508, Nashville, TN 37217. 615-781-5770. SUPPLEMENTARY INFORMATION: The FHWA, in cooperation with the Tennessee... this proposed program.) Charles J. O'Neill, Planning and Program Mgmt., Team Leader, Nashville, TN. [FR...

The development of a hydrologic-hydraulic representation of an urbanscape: the case study of Nashville, Tennessee

NASA Astrophysics Data System (ADS)

Sedlar, F.; Ivanov, V. Y.; Shao, J.; Narayan, U.; Nardi, F.; Adams, T. E.; Merwade, V.; Wright, D. B.; Kim, J.; Fatichi, S.; Rakhmatulina, E.

2013-12-01

Incorporating elevation data into coupled hydraulic and hydrologic models with the use of triangulated irregular networks (TINs) provides a detailed and highly customizable representation of the original domain. Until recently the resolution of such digital elevation models was 1 or 1/3 arc second (10-30 meters). Aided by the use of LiDAR, digital elevation models are now available at the 1/9 arc second resolution (1-3 meters). With elevation data at this level of resolution watershed details that are overlooked at a 10-30 meter resolution can now be resolved and incorporated into the TIN. For urban flood modeling this implies that street level features can be resolved. However to provide a useful picture of the flooding as a whole, this data would need to be integrated across a citywide scale. To prove the feasibility, process, and capabilities of generating such a detailed and large scale TIN, we present a case study of Nashville, TN, USA, during the May 1-2, 2010 flooding, a 1,000 year storm event. With the use of ArcGIS, HEC-RAS, Triangle, and additionally developed processing methodologies, an approach is developed to generate a hydrologically relevant and detailed TIN of the entire urbanscape of Nashville. This TIN incorporates three separate aspects; the watershed, the floodplain, and the city. The watershed component contains the elevation data for the delineated watershed, roughly 1,000 km2 at 1-3 meter resolution. The floodplain encompasses over 300 channel cross sections of the Cumberland River and a delineated floodplain. The city element comprises over 500,000 buildings and all major roadways within the watershed. Once generated, the resulting triangulation of the TIN is optimized with the Triangle software for input to the coupled hydraulic and hydrological model, tRIBS-OFM. Hydrologically relevant areas such as the floodplain are densified and constraints are set on the minimum triangle area for the entire TIN. Upon running the coupled hydraulic and

76 FR 3189 - Notice to Rescind a Notice of Intent to Prepare a Supplemental Environmental Impact Statement...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-19

... Management Team Leader, FHWA--Tennessee Division Office, 404 BNA Drive-Suite 508 Nashville, TN 37217. Phone: (615) 781-5770. SUPPLEMENTARY INFORMATION: The FHWA, in cooperation with the Tennessee Department of... program.) Charles J. O'Neill, Planning and Program Management Team Leader, Nashville, TN. [FR Doc. 2011...

Assessing Effectiveness of Students Taking a Right Stand (STARS) Nashville Student Assistance Programs

ERIC Educational Resources Information Center

Kanu, Mohamed; Hepler, Nancy; Labi, Halima

2015-01-01

Background: Since 1984, Students Taking a Right Stand (STARS) Nashville has implemented Student Assistance Programs (SAPs) in the middle Tennessee area, to include 14 counties and 16 school districts. STARS Nashville serves K-12 with a focus in middle and high schools. Methods: The current study reviewed studies that utilized quasi-experimental…

76 FR 60802 - Foreign-Trade Zone 77-Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-30

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket T-4-2011] Foreign-Trade Zone 77--Memphis, TN; Withdrawal of Request for Temporary/Interim Manufacturing Authority; Flextronics Logistics USA, Inc., (Cell Phone/Mobile Handset Kitting). Memphis, TN Notice is hereby given of the withdrawal of the...

NASHVILLE, EXPERIMENT IN URBAN SCHOOL CONSOLIDATION.

ERIC Educational Resources Information Center

EGERTON, JOHN; LEESON, JIM

THE BIGGEST CHANGE WHICH RESULTED FROM THE CONSOLIDATION OF NASHVILLE AND DAVIDSON COUNTY, TENNESSEE, INTO ONE GOVERNMENTAL UNIT WAS THE CREATION OF A UNIFIED SCHOOL SYSTEM. NOW, ALL BUT ONE MEMBER OF THE NEW METRO BOARD OF EDUCATION ARE NEW APPOINTEES, AS IS THE DIRECTOR OF THE SCHOOL SYSTEM. UNDER THIS NEW LEADERSHIP BROAD CHANGES IN THE…

78 FR 68699 - IFR Altitudes; Miscellaneous Amendments

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-15

... MAROC, IL FIX *3000 *2400--MOCA MAROC, IL FIX PONTIAC, IL VOR/DME..... 2500 Sec. 95.6066 VOR Federal... NASHVILLE, TN VORTAC *LENON, TN FIX **3000 *6500--MRA **2400--MOCA *LENON, TN FIX HARME, TN FIX **3000 [[Page 68701

ACTE Convention a Big Success in Nashville

ERIC Educational Resources Information Center

Techniques: Connecting Education and Careers (J1), 2010

2010-01-01

This article features the 2009 Association for Career and Technical Education's (ACTE) Annual Convention and Career Tech Expo which was held in Nashville in November. Despite somber economic times, the Annual Convention drew thousands of attendees, more than 200 exhibitors, offered engaging sessions and speakers, and the new chief of the Office of…

Changing Economics in the South: Preparing Our Youth. Hearing before the Select Committee on Children, Youth, and Families. House of Representatives, One Hundredth Congress, First Session (Nashville, TN, April 24, 1987).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House Select Committee on Children, Youth, and Families.

The House Select Committee on Children, Youth, and Families met in Nashville, Tennessee, to gather information on the education and employment futures of youth in the South. Testimony was heard from experts in education, training, and economic development. Organizations represented included universities in the region, adolescent dropout and…

Molecular dynamics studies on troponin (TnI-TnT-TnC) complexes: insight into the regulation of muscle contraction.

PubMed

Varughese, Jayson F; Chalovich, Joseph M; Li, Yumin

2010-10-01

Mutations of any subunit of the troponin complex may lead to serious disorders. Rational approaches to managing these disorders require knowledge of the complex interactions among the three subunits that are required for proper function. Molecular dynamics (MD) simulations were performed for both skeletal (sTn) and cardiac (cTn) troponin. The interactions and correlated motions among the three components of the troponin complex were analyzed using both Molecular Mechanics-Generalized Born Surface Area (MMGBSA) and cross-correlation techniques. The TnTH2 helix was strongly positively correlated with the two long helices of TnI. The C domain of TnC was positively correlated with TnI and TnT. The N domain of TnC was negatively correlated with TnI and TnT in cTn, but not in sTn. The two C-domain calcium-binding sites of TnC were dynamically correlated. The two regulatory N-domain calcium-binding sites of TnC were dynamically correlated, even though the calcium-binding site I is dysfunctional. The strong interaction residue pairs and the strong dynamically correlated residues pairs among the three components of troponin complexes were identified. These correlated motions are consistent with the idea that there is a high degree of cooperativity among the components of the regulatory complex in response to Ca(2+) and other effectors. This approach may give insight into the mechanism by which mutations of troponin cause disease. It is interesting that some observed disease causing mutations fall within regions of troponin that are strongly correlated or interacted.

Identification of multiple mercury sources to stream sediments near Oak Ridge, TN, USA

DOE PAGES

Donovan, Patrick M.; Blum, Joel D.; Demers, Jason D.; ...

2014-03-03

In this paper, sediments were analyzed for total Hg concentration (THg) and isotopic composition from streams and rivers in the vicinity of the Y-12 National Security Complex (Y12) in Oak Ridge, TN (USA). In the stream directly draining Y12, where industrial releases of mercury (Hg) have been documented, high THg (3.26 to 60.1 μg/g) sediments had a distinct Hg isotopic composition (δ 202Hg of 0.02 ± 0.15‰ and Δ 199Hg of -0.07 ± 0.03‰; mean ± 1SD, n=12) compared to sediments from relatively uncontaminated streams in the region (δ 202Hg = -1.40 ± 0.06‰ and Δ 199Hg of –0.26 ±more » 0.03‰; mean ± 1SD, n=6). Additionally, several streams that are nearby but do not drain Y12 had sediments with intermediate THg (0.06 to 0.21 μg/g) and anomalous δ 202Hg (as low as -5.07‰). We suggest that the low δ 202Hg values in these sediments provide evidence for the contribution of an additional Hg source to sediments, possibly derived from atmospheric deposition. In sediments directly downstream of Y12 this third Hg source is not discernible and the Hg isotopic composition can be largely explained by the mixing of low THg sediments with high THg sediments contaminated by Y12 discharges.« less

76 FR 5078 - Approval and Promulgation of Air Quality Implementation Plans: Tennessee; Approval of Section 110...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-28

...) Maintenance Plan for the 1997 8-Hour Ozone Standards for the Nashville, TN, Area AGENCY: Environmental... Tennessee State Implementation Plan (SIP) concerning the maintenance plan addressing the 1997 8-hour ozone standards for the Nashville, Tennessee 1997 8-hour ozone maintenance area, which is comprised of Davidson...

Leading by Example: Principal Leadership Institutes as a Driver for Change in Metro Nashville Public Schools. Executive Summary

ERIC Educational Resources Information Center

Raynor, Alethea Frazier; Potochnik, Tracie; Thompson, Joanne; Dowcett, Kathleen

2015-01-01

In 2009, Metropolitan Nashville Public Schools (MNPS) was on the brink of state takeover, with low academic performance, significant achievement gaps, and a number of schools failing to meet performance targets under No Child Left Behind. Additionally, over the past decade, significant demographic shifts in Nashville have resulted in MNPS becoming…

76 FR 27169 - Notice of Passenger Facility Charge (PFC) Approvals and Disapprovals

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-10

.../11 27,518,418 20,833,179 01/01/16 06/01/16 Nashville, TN 03-03-C-01-SAN 2/17/11 83,075,730 65,058,035 03/01/06 03/01/06 San Diego, CA 09-15-C-01-BNA 2/17/11 11,287,500 6,196,434 06/01/17 09/01/16 Nashville, TN 06-07-C-06-BUR 2/18/11 41,346,265 42,946,265 04/01/13 06/01/13 Burbank, CA 09-09-C-01-BUR 2/18...

COLLEGIUM INTERNATIONALE NEURO-PSYCHOPHARMACOLOGICUM (C.I.N.P.) 24th CONGRESS (Paris, France, 20-24 June 2004).

PubMed

Pivac, Nela

2004-09-01

The 24th C.I.N.P. meeting was held in Paris, France, from June 20-24, 2004. The opening ceremony and welcome reception was chaired by Professor Herbert Y Meltzer, Vanderbilt University, Nashville, TN, USA, President of C.I.N.P., and a new president was elected (Professor Brian E. Leonard, Ireland). Congress included the rich social program, combined with lectures of invited speakers, speeches and awards. There were 6650 registered participants all over the world. The meeting consisted of 4 plenary lectures (held by Professor FE Bloom, Department of Neuropharmacology, The Scripps Research Institute, La Jolla USA, Professor HY Meltzer, Vanderbilt University, Nashville, TN, USA, Professor CL Masters, The University of Melbourne, and the Mental Health Research Institute of Victoria, Melbourne, Australia, and Professor JP Changeux, CNRS URA 2182 Recepteurs and Cognition, Paris, France), 69 symposia, 5 synthesia, 25 satellite symposia, 2 electronic interactive sessions, 6 meet the expert sessions, 11 sessions that were held under the name breaking scientific news, 6 workshops, 5 joint meetings, and 2 poster sessions with 657 posters. Posters were displayed for 2 days between 12.00 to 18.00 h, and presenters were available between 16.30 and 18.00 h to answer the questions. Abstracts from the congress were published in International Journal of Neuropsychopharmacology, vol 7, Supplement 1, June 2004. The 24th Collegium Internationale Neuro-Psychopharmacologicum (C.I.N.P.) Congress introduced some new exciting data, summarized some new knowledge, and its goal was to connect the preclinical and clinical data and to introduce some news into clinical practice. The smaller part of the congress that I was able to follow was well organized, and very good attended, with diverse topics, covering all aspects of neuropsychopharmacological research. The sponsorship by the pharmaceutical industries was visible only in the selected sponsored symposia.

Genetic and molecular studies of a composite chromosomal element (Tn3705) containing a Tn916-modified structure (Tn3704) in Streptococcus anginosus F22.

PubMed

Clermont, D; Horaud, T

1994-01-01

The plasmid-free Streptococcus anginosus F22 contained a conjugative element, Tn3705, encoding resistance to erythromycin (Emr) and tetracycline-minocycline (Tcr-Mnr). We mapped a chromosomal region (> 52 kb) of F22, corresponding to the internal part of Tn3705. Molecular analysis of Tn3705 revealed it to be a composite structure: it included in its central part a transposon designated Tn3704 (20.3 kb +/- 0.5 kb), which had a modified structure in comparison with that of Tn916 and on which the Emr Tcr-Mn4 markers were localized. Tn3705 inserted from F22 into the chromosome of various streptococcal transconjugants as well as that of Enterococcus faecalis transconjugants without changing its structure. In contrast, from the chromosome of an E. faecalis::Tn3705 transconjugant only Tn3704 inserted, at various sites, into another E. faecalis chromosome. Sugar fermentations occurred after the insertion of Tn3704 into the chromosome of an asaccharolytic E. faecalis strain. Transposition of only Tn3704 from the chromosome of E. faecalis::Tn3705 onto pIP964, an E. faecalis hemolysin plasmid, yielded two different pIP964 derivatives. The size of the entire element Tn3705 was estimated to be about 70.0 kb by pulsed-field electrophoresis.

New Techniques for Real-Time Stage Forecasting for Tributaries in the Nashville Area

NASA Astrophysics Data System (ADS)

Charley, W.; Moran, B.; LaRosa, J.

2011-12-01

On Saturday, May 1, 2010, heavy rain began falling in the Cumberland River Valley, Tennessee, and continued through the following day. 13.5 inches was measured at Nashville, an unprecedented amount that doubled the previous 2-day record, and exceeded the May monthly total record of 11 inches. Elsewhere in the valley, amounts of over 19 inches were measured. This intensity of rainfall quickly overwhelmed tributaries to the Cumberland in the Nashville area, causing wide-spread and serious flooding. Tractor-trailers and houses were seen floating down Mill Creek, a primary tributary in the south eastern area of Nashville. Twenty-six people died and over 2 billion dollars in damage occurred as a result of the flood. Since that time, several other significant rainfall events have occurred in the area. As a result of the flood, agencies in the Nashville area want better capabilities to forecast stages for the local tributaries. Better stage forecasting will help local agencies close roads, evacuate homes and businesses and similar actions. An interagency group, consisting of Metro Nashville Water Services and Office of Emergency Management, the National Weather Service, the US Geological Survey and the US Army Corps of Engineers, has been established to seek ways to better forecast short-term events in the region. It should be noted that the National Weather Service has the official responsibility of forecasting stages. This paper examines techniques and algorithms that are being developed to meet this need and the practical aspects of integrating them into a usable product that can quickly and accurately forecast stages in the short-time frame of the tributaries. This includes not only the forecasting procedure, but also the procedure to acquire the latest precipitation and stage data to make the forecasts. These procedures are integrated into the program HEC-RTS, the US Army Corps of Engineers Real-Time Simulation program. HEC-RTS is a Java-based integration tool that

31 CFR 100.17 - Location of Federal Reserve banks and branches.

Code of Federal Regulations, 2010 CFR

2010-07-01

...), Miami, FL 33152 Nashville Branch—301 Eighth Avenue, North, Nashville, TN 37203 New Orleans Branch—525 St. Charles Avenue (P.O. Box 61630), New Orleans, LA 70161 Chicago—230 South LaSalle Street (P.O. Box 834... branches. Federal Reserve Bank and Address Boston—600 Atlantic Avenue, Boston, MA 02106 New York—33 Liberty...

76 FR 53115 - Foreign-Trade Zone 77-Memphis, TN; Application for Temporary/Interim Manufacturing Authority...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-25

..., TN; Application for Temporary/ Interim Manufacturing Authority; Flextronics Logistics USA, Inc. (Cell... authority to produce cell phones/mobile handsets kits (HTSUS 8517.12, HTSUS 8517.62, HTSUS 8517.69, duty... cell phone/mobile handset kits (duty free) for the foreign inputs noted above. In accordance with the...

[Construction of cTnC-linker-TnI (P) Genes, Expression of Fusion Protein and Preparation of Lyophilized Protein].

PubMed

Song, Xiaoli; Liu, Xiaoyun; Cai, Lei; Wu, Jianwei; Wang, Jihua

2015-12-01

In order to construct and express human cardiac troponin C-linker-troponin I(P) [ cTnC-linker-TnI(P)] fusion protein, detect its activity and prepare lyophilized protein, we searched the CDs of human cTnC and cTnI from GenBank, synthesized cTnC and cTnI(30-110aa) into cloning vector by a short DNA sequence coding for 15 neutral amino acid residues. pCold I-cTnC-linker-TnI(P) was constructed and transformed into E. coli BL21(DE3). Then, cTnC-linker-TnI(P) fusion protein was induced by isopropyl-β-D-thiogalactopyranoside (IPTG). Soluable expression of cTnC-linker-TnI(P) in prokaryotic system was successfully obtained. The fusion protein was purified by Ni²⁺ Sepharose 6 Fast Flow affinity chromatography with over 95% purity and prepared into lyophilized protein. The activity of purified cTnC-linker-TnI(P) and its lyophilized protein were detected by Wondfo Finecare™ cTnI Test. Lyophilized protein of cTnC-linker-TnI(P) was stable for 10 or more days at 37 °C and 4 or more months at 25 °C and 4 °C. The expression system established in this research is feasible and efficient. Lyophilized protein is stable enough to be provided as biological raw materials for further research.

Comparing different scientific approaches to personalized medicine: research ethics and privacy protection.

PubMed

Langanke, Martin; Brothers, Kyle B; Erdmann, Pia; Weinert, Jakob; Krafczyk-Korth, Janina; Dörr, Marcus; Hoffmann, Wolfgang; Kroemer, Heyo K; Assel, Heinrich

2011-07-01

In this article, two different scientific approaches to personalized medicine are compared. Biorepository at Vanderbilt University (BioVU) is a genomic biorepository at Vanderbilt University Medical Center in Nashville, TN, USA. Genetic biosamples are collected from leftover clinical blood samples; medical information is derived from an electronic medical records. Greifswald Approach to Individualized Medicine is a research resource at the University of Greifswald, Germany, comprised of clinical records combined with biosamples collected for research. We demonstrate that although both approaches are based on the collection of clinical data and biosamples, different legal milieus present in the USA and Germany as well as slight differences in scientific goals have led to different 'ethical designs'. While BioVU can successfully operate with an 'opt-out' mechanism, an informed consent-based 'opt-in' model is indispensable to allow GANI_MED to reach its scientific goals.

Books for Black Children: Public Library Collections in Louisville and Nashville, 1915-1925.

ERIC Educational Resources Information Center

Malone, Cheryl Knott

2000-01-01

Describes the racially segregated public library children's collections and services in Louisville, Kentucky and Nashville, Tennessee. Discusses the relationship between African American librarians and users based on original library records, annual reports, and librarians' writings; describes and evaluates the collections developed for black…

Challenges in Antibody Development against Tn and Sialyl-Tn Antigens

PubMed Central

Loureiro, Liliana R.; Carrascal, Mylène A.; Barbas, Ana; Ramalho, José S.; Novo, Carlos; Delannoy, Philippe; Videira, Paula A.

2015-01-01

The carbohydrate antigens Tn and sialyl-Tn (STn) are expressed in most carcinomas and usually absent in healthy tissues. These antigens have been correlated with cancer progression and poor prognosis, and associated with immunosuppressive microenvironment. Presently they are used in clinical trials as therapeutic vaccination, but with limited success due to their low immunogenicity. Alternatively, anti-Tn and/or STn antibodies may be used to harness the immune system against tumor cells. Whilst the development of antibodies against these antigens had a boost two decades ago for diagnostic use, so far no such antibody entered into clinical trials. Possible limitations are the low specificity and efficiency of existing antibodies and that novel antibodies are still necessary. The vast array of methodologies available today will allow rapid antibody development and novel formats. Following the advent of hybridoma technology, the immortalization of human B cells became a methodology to obtain human monoclonal antibodies with better specificity. Advances in molecular biology including phage display technology for high throughput screening, transgenic mice and more recently molecularly engineered antibodies enhanced the field of antibody production. The development of novel antibodies against Tn and STn taking advantage of innovative technologies and engineering techniques may result in innovative therapeutic antibodies for cancer treatment. PMID:26270678

Tn5Prime, a Tn5 based 5' capture method for single cell RNA-seq.

PubMed

Cole, Charles; Byrne, Ashley; Beaudin, Anna E; Forsberg, E Camilla; Vollmers, Christopher

2018-06-01

RNA-sequencing (RNA-seq) is a powerful technique to investigate and quantify entire transcriptomes. Recent advances in the field have made it possible to explore the transcriptomes of single cells. However, most widely used RNA-seq protocols fail to provide crucial information regarding transcription start sites. Here we present a protocol, Tn5Prime, that takes advantage of the Tn5 transposase-based Smart-seq2 protocol to create RNA-seq libraries that capture the 5' end of transcripts. The Tn5Prime method dramatically streamlines the 5' capture process and is both cost effective and reliable. By applying Tn5Prime to bulk RNA and single cell samples, we were able to define transcription start sites as well as quantify transcriptomes at high accuracy and reproducibility. Additionally, similar to 3' end-based high-throughput methods like Drop-seq and 10× Genomics Chromium, the 5' capture Tn5Prime method allows the introduction of cellular identifiers during reverse transcription, simplifying the analysis of large numbers of single cells. In contrast to 3' end-based methods, Tn5Prime also enables the assembly of the variable 5' ends of the antibody sequences present in single B-cell data. Therefore, Tn5Prime presents a robust tool for both basic and applied research into the adaptive immune system and beyond.

Aircraft accident report : ground spoiler activation in flight/hard landing Valujet Airlines Flight 558, Douglas DC-9-32, N922VV Nashville, Tennessee, January 7, 1996

DOT National Transportation Integrated Search

1996-12-11

Adopted: December 11,1996. Notation 6781. This report explains the ground spoiler activation in flight and subsequent hard landing of ValuJet Airlines flight 558, N922VV, a Douglas DC-9-32 at Nashville International Airport, Nashville, Tennessee. The...

Ozone production during an urban air stagnation episode over Nashville, Tennessee

NASA Astrophysics Data System (ADS)

Valente, R. J.; Imhoff, R. E.; Tanner, R. L.; Meagher, J. F.; Daum, P. H.; Hardesty, R. M.; Banta, R. M.; Alvarez, R. J.; McNider, R. T.; Gillani, N. V.

1998-09-01

The highest O3 levels observed during the 1995 Southern Oxidants Study in middle Tennessee occurred during a period of air stagnation from July 11 through July 15. Extensive airborne (two fixed wing and one helicopter) and ground-based measurements of the chemistry and meteorology of this episode near Nashville, Tennessee, are presented. In situ airborne measurements include O3, NOy, NO, NO2, SO2, CO, nitrate, hydrocarbons, and aldehydes. Airborne LIDAR O3 measurements are also utilized to map the vertical and horizontal extent of the urban plume. The use of multiple instrumented research aircraft permitted highly detailed mapping of the plume chemistry in the vertical and horizontal dimensions. Interactions between the urban Nashville plume (primarily a NOx and hydrocarbon source) and the Gallatin coal-fired power plant plume (primarily a NOx and SO2 source) are also documented, and comparisons of ozone formation in the isolated and mixed urban and power plant plume are presented. The data suggest that during this episode the background air and the edges of the urban plume are NOx sensitive and the core of the urban plume is hydrocarbon sensitive. Under these worst case meteorological conditions, ambient O3 levels well over the level of the new National Ambient Air Quality Standard (NAAQS) for ozone (80 ppb) were observed over and just downwind of Nashville. For example, on July 12, the boundary layer air upwind of Nashville showed 60 to 70 ppb O3, while just downwind of the city the urban plume maximum was over 140 ppb O3. With a revised ozone standard set at 80 ppb (8 hour average) and upwind levels already within 10 or 20 ppb of the standard, only a slight increase in ozone from the urban area will cause difficulty in attaining the standard at monitors near the core of the urban plume during this type of episode. The helicopter mapping and LIDAR aircraft data clearly illustrate that high O3 levels can occur during stagnation episodes within a few kilometers of

Model Integration for Real-Time Flood Forecasting Inundation Mapping for Nashville Tributaries

NASA Astrophysics Data System (ADS)

Charley, W.; Moran, B.; LaRosa, J.

2012-12-01

In May of 2010, between 14 and 19 inches of rain fell on the Nashville metro area in two days, quickly overwhelming tributaries to the Cumberland River and causing wide-spread, serious flooding. Tractor-trailers and houses were seen floating down Mill Creek, a primary tributary in the south eastern area of Nashville. Twenty-six people died and over 2 billion dollars in damage occurred as a result of the flood. Since that time, several other significant rainfall events have occurred in the area. Emergency responders were unable to deliver aid or preventive measures to areas under threat of flooding (or under water) in time to reduce damages because they could not identify those areas far enough in advance of the floods. Nashville Metro Water, the National Weather Service, the US Geological Survey and the US Army Corps of Engineers established a joint venture to seek ways to better forecast short-term flood events in the region. One component of this effort was a pilot project to compute and display real time inundation maps for Mill Creek, a 108 square-mile basin to the south east of Nashville. HEC-RTS (Real-Time Simulation) was used to assimilate and integrate the hydrologic model HEC-HMS with the hydraulics model HEC-RAS and the inundation mapping program HEC-RAS Mapper. The USGS, along with the other agencies, installed additional precipitation and flow/stage gages in the area. Measurements are recorded every 5-30 minutes and are posted on the USGS NWIS database, which are downloaded by HEC-RTS. Using this data in combination with QPFs (Quantitative Precipitation Forecasts) from the NWS, HEC-RTS applies HEC-HMS and HEC-RAS to estimate current and forecast stage hydrographs. The peak stages are read by HEC-RAS Mapper to compute inundation depths for 6 by 6 foot grid cells. HEC-RTS displays the inundation on a high resolution MrSid aerial photo, along with subbasin boundary, street and various other layers. When a user zooms in and "mouses" over a cell, the

Comparison of linear cephalometric dimensions in Americans of European descent (Ann Arbor, Cleveland, Philadelphia) and Americans of African descent (Nashville).

PubMed

Dibbets, Jos M H; Nolte, Kai

2002-08-01

Eleven dimensions, extracted from four commercially available cephalometric atlases were compared. Three populations were American of European descent and one was American of African descent. The source data were carefully corrected for linear enlargement. The confounding effect of linear radiographic enlargement is exemplified by depicting the often-used distance, sella-nasion, before and after correction. Total face height was smallest in the Cleveland population and largest in the Nashville population. The difference was fully accounted for by differences in lower face height and that was the most variable of all dimensions studied. Upper face height was almost identical in all four populations. Posterior face height was largest in the Nashville population. The mandible in the Nashville population had an average ramus height, but a longer corpus. Mandibular dimensions were equal in the three other populations. The maxilla was clearly shortest in the Cleveland population and almost of equal length in the three others.

Use of the Delphi method for determining community growth goals inventory: the Nashville experience

Treesearch

Vishwa K. Varma

1977-01-01

The author discusses the growth-inducing pressures on Nashville, Tennessee, describes the application of the Delphi technique to develop an inventory of the community's growth goals, and suggests that the development of a list of community goals is a necessary first step toward growth management.

Comparing different scientific approaches to personalized medicine: research ethics and privacy protection

PubMed Central

Langanke, Martin; Brothers, Kyle B; Erdmann, Pia; Weinert, Jakob; Krafczyk-Korth, Janina; Dörr, Marcus; Hoffmann, Wolfgang; Kroemer, Heyo K; Assel, Heinrich

2011-01-01

In this article, two different scientific approaches to personalized medicine are compared. Biorepository at Vanderbilt University (BioVU) is a genomic biorepository at Vanderbilt University Medical Center in Nashville, TN, USA. Genetic biosamples are collected from leftover clinical blood samples; medical information is derived from an electronic medical records. Greifswald Approach to Individualized Medicine is a research resource at the University of Greifswald, Germany, comprised of clinical records combined with biosamples collected for research. We demonstrate that although both approaches are based on the collection of clinical data and biosamples, different legal milieus present in the USA and Germany as well as slight differences in scientific goals have led to different ‘ethical designs’. While BioVU can successfully operate with an ‘opt-out’ mechanism, an informed consent-based ‘opt-in’ model is indispensable to allow GANI_MED to reach its scientific goals. PMID:21892358

Incised valley fill interpretation for Mississippian Black Hand Sandstone, Appalachian Basin, USA: Implications for glacial eustasy at Kinderhookian-Osagean (Tn2-Tn3) boundary

USGS Publications Warehouse

Matchen, D.L.; Kammer, T.W.

2006-01-01

Lower Mississippian strata of east-central Ohio are predominantly fine-grained marine deposits of the Cuyahoga and Logan formations. Within these sediments is the Black Hand Sandstone of the Cuyahoga Formation. The Black Hand Sandstone is a multistory, crossbedded, coarse-grained conglomeratic sandstone. The contact between the Black Hand Sandstone and the subjacent Cuyahoga Formation is sharp and scoured, with intraclasts of the Cuyahoga Formation incorporated into the basal Black Hand Sandstone. The Black Hand Sandstone was previously thought to represent a distributary channel deposit; however, the combination of lithofacies and architectural elements indicates deposition in a braided stream setting. The Cuyahoga Formation was deposited in a shallow marine setting. The erosional basal contact of the Black Hand Sandstone and the juxtaposition of fluvial and marine sediments suggests a sequence boundary. The geographic distribution of the Black Hand Sandstone combined with the evidence for a sequence boundary suggests deposition in an incised valley. The age of the Black Hand Sandstone is key to inferring the causes of valley incision. The Black Hand Sandstone is nearly devoid of body fossils, necessitating a biostratigraphic analysis of the surrounding Cuyahoga and Logan formations. Analysis indicates the Logan Formation is early Osagean age. Data from the Cuyahoga Formation suggest a Kinderhookian age with a possible transition to the Osagean in the uppermost Cuyahoga Formation. This constrains the age of the Black Hand Sandstone to the transition at the Kinderhookian-Osagean boundary. Recent reports indicate late Kinderhookian (Tournaisian, Tn2) Gondwanan glaciation based upon tillites and sharp excursions in stable-isotope curves. A glacio-eustatic fall in sea level is inferred to have caused incision of the Cuyahoga Formation, followed by deposition of the Black Hand Sandstone and Logan Formation during the subsequent sea level rise. The associated

LABORATORY ANALYSES: WATER AND ENVIRONMENTAL SAMPLES

EPA Science Inventory

To be presented at the Workshop for Improving the Recognition, Investigation, and Reporting of Waterborne Disease Outbreaks Associated with Drinking, Recreational and Other Waters in Nashville, TN, May 29 - June 1, 2007

7. Photograph of a line drawing. 'PART III, SECTION 1, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

7. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDING NO. 10, PRODUCER GAS & EXHAUSTER BLDG., PLANT A.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant A, Parts I, II, III. (Nashville, TN: Office of District Engineer, 1944). - Holston Army Ammunition Plant, Producer Gas Plant, Kingsport, Sullivan County, TN

Flood-inundation maps for North Fork Salt Creek at Nashville, Indiana

USGS Publications Warehouse

Martin, Zachary W.

2017-11-13

Digital flood-inundation maps for a 3.2-mile reach of North Fork Salt Creek at Nashville, Indiana, were created by the U.S. Geological Survey (USGS) in cooperation with the Indiana Department of Transportation. The flood-inundation maps, which can be accessed through the USGS Flood Inundation Mapping Science website at http://water.usgs.gov/osw/flood_inundation/, depict estimates of the areal extent and depth of flooding that correspond to selected water levels (stages) at the North Fork Salt Creek at Nashville, Ind., streamgage (USGS station number 03371650). Real-time stages at this streamgage may be obtained from the USGS National Water Information System at http://waterdata.usgs.gov/nwis or the National Weather Service (NWS) Advanced Hydrologic Prediction Service at http:/water.weather.gov/ahps/, which also shows observed USGS stages at the same site as the USGS streamgage (NWS site NFSI3).Flood profiles were computed for the stream reach by means of a one-dimensional, step-backwater hydraulic modeling software developed by the U.S. Army Corps of Engineers. The hydraulic model was calibrated using the current (2015) stage-discharge rating at the USGS streamgage 03371650, North Fork Salt Creek at Nashville, Ind. The hydraulic model was then used to compute 12 water-surface profiles for flood stages at 1-foot (ft) intervals, except for the highest profile of 22.9 ft, referenced to the streamgage datum ranging from 12.0 ft (the NWS “action stage”) to 22.9 ft, which is the highest stage of the current (2015) USGS stage-discharge rating curve and 1.9 ft higher than the NWS “major flood stage.” The simulated water-surface profiles were then combined with a geographic information system digital elevation model (derived from light detection and ranging data having a 0.98-ft vertical accuracy and 4.9-ft horizontal resolution) to delineate the area flooded at each stage.The availability of these maps, along with information regarding current stage from the USGS

21. Historic American Buildings Survey, W. Jeter Eason Deputy District ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

21. Historic American Buildings Survey, W. Jeter Eason- Deputy District Officer, Photographer November 21, 1936 DETAIL OF DECORATIVE STAIR RISERS. - The Hermitage, U.S. Highway 70 North (4580 Rachel's Lane), Nashville, Davidson County, TN

IMPACT of City-County Consolidation of the Rural-Urban Fringe: Nashville-Davidson County, Tennessee.

ERIC Educational Resources Information Center

Economic Research Service (USDA), Washington, DC.

This report analyzed the effect of consolidation of city and county governments in Nashville, Tenn., on local public finance and the availability of public services in the rural areas of the county. Comparisons were made between governmental costs and functions before and 3 years after the metropolitan district was formed. Some 299 voters in the…

Breaking the Cycle of Inequitable School Discipline through Community and Civic Collaboration in Nashville

ERIC Educational Resources Information Center

Majors, Tony; Ward, Tom

2015-01-01

Tom Ward and Tony Majors, community and district lead partners in Nashville, Tennessee, talk about what the Positive and Safe Schools Advancing Greater Equity (PASSAGE) initiative has meant in their city. They share how and why their journey began by embedding the work to end discipline disparities across a broad, cross-sector table that includes…

LOW DOSE PESTICIDE EXPOSURE AND ALTERED REPRODUCTIVE SYSTEM DEVELOPMENT IN REPTILES

EPA Science Inventory

In: Environmental Sciences in the 21st Century: Paradigms, Opportunities, and Challenges: Abstract Book: SETAC 21st Annual Meeting, 12-16 November 2000, Nashville, TN. Society of Environmental Toxicology and Chemistry, Pensacola, FL. Pp. 99.

38. Photograph of a line drawing. 'PART III, SECTION 1, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

38. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS G-1 TO G-10 INCL., PURIFICATION, MANUFACTURING AREA, PLAN 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

43. Photograph of a line drawing. 'PART III, SECTION 1, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

43. Photograph of a line drawing. 'PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

75 FR 21384 - Culturally Significant Objects Imported for Exhibition Determinations: “Birth of Impressionism...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-23

... Visual Arts, Nashville, TN, from on or about October 15, 2010, until January 23, 2011, and at possible... that the exhibition or display of the exhibit objects at the Fine Arts Museums of San Francisco, San...

INTER-SPECIES STEROID RECEPTOR EXTRAPOLATION STUDIES FOR ENDOCRINE DISRUPTING CHEMICALS

EPA Science Inventory

In: Environmental Sciences in the 21st Century: Paradigms, Opportunities, and Challenges: Abstract Book: SETAC 21st Annual Meeting, 12-16 November 2000, Nashville, TN. Society of Environmental Toxicology and Chemistry, Pensacola, FL. Pp. 117-118.

77 FR 12102 - Culturally Significant Objects Imported for Exhibition Determinations: “Constable: Oil Sketches...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-28

... the Princeton University Art Museum, Princeton, NJ, from, on or about March 17, 2012, until on or about June 10, 2012; the Frist Center for the Visual Arts, Nashville, TN, from on or about June 22, 2012...

Nashville Solar-Water-Heater Demonstration Project. Monitoring-data analysis

NASA Astrophysics Data System (ADS)

1982-03-01

Field monitoring data which were collected for the Nashville Solar Water Heater Demonstration Project from September through November of 1981 are presented. Twenty-six solar domestic water heaters were monitored during September, 35 during October, and 37 during November. Homeowners were audited to assure adequate solar access, and each selected a solar water heating system from an approved list. Two tank and one tank systems are included. The monitoring sample technique and monitoring system are described. Data are analyzed by computer to produce daily and monthly total summaries for each site. The performance of each site was assessed to compare total energy saved by the solar system, solar system savings percentage, and the energy multiplier.

How Busing Burdened Blacks: Critical Race Theory and Busing for Desegregation in Nashville-Davidson County

ERIC Educational Resources Information Center

Woodward, Jennifer R.

2011-01-01

This article uses critical race theory, court opinions, newspapers, and interviews to explain how the burden of busing for desegregation was placed upon Blacks in Nashville, Tennessee and why the agenda of the litigants in the Kelley v. Metropolitan Board of Education cases shifted over time. The deliberate pace of the initial desegregation…

44. Photograph of a line drawing. 'PLAN LAYOUT OF PART ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

44. Photograph of a line drawing. 'PLAN LAYOUT OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant 8, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

31. Photograph of a line drawing. 'PLAN LAYOUT OF PART ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

31. Photograph of a line drawing. 'PLAN LAYOUT OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS D-1 TO U-10 INCL., NITRATION, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

30. Photograph of a line drawing. 'CROSS SECTION OF PART ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

30. Photograph of a line drawing. 'CROSS SECTION OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS D-1 TO D-10 INCL., NITRATION, MANUFACTURING AREA, PLANT 'B'.' From U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

78 FR 2659 - Application(s) for Duty-Free Entry of Scientific Instruments

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-14

..., 2201 West End Ave., Nashville, TN 37235. Instrument: Electron Microscope. Manufacturer: FEI Company... St., West Lafayette, IN 47907-2024. Instrument: Electron Microscope. Manufacturer: FEI Company, the..., microorganisms, nanomaterials, and chemical compounds. Justification for Duty-Free Entry: There are no...

24. Historic American Buildings Survey, W. Jeter Eason Deputy District ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

24. Historic American Buildings Survey, W. Jeter Eason- Deputy District Officer, Photographer November 21, 1936 PLASTER ORNAMENT ON CEILING AND PLASTER CORNICE BACK PARLOR (FRONT PARLOR SIMLAR). - The Hermitage, U.S. Highway 70 North (4580 Rachel's Lane), Nashville, Davidson County, TN

ENVIRONMENTAL SCIENCES IN THE 21ST CENTURY: PARADIGMS, OPPORTUNITIES, AND CHALLENGES

EPA Science Inventory

In: Environmental Sciences in the 21st Century: Paradigms, Opportunities, and Challenges: Abstract Book: SETAC 21st Annual Meeting, 12-16 November 2000, Nashville, TN.. Society of Environmental Toxicology and Chemistry, Pensacola, FL. Pp. p. 180.

Bioavailability and rates...

IN VITRO ANDROGENIC ACTIVITY OF KRAFT MILL EFFLUENT

EPA Science Inventory

In: Environmental Sciences in the 21st Century: Paradigms, Opportunities, and Challenges: Abstract Book: SETAC 21st Annual Meeting, 12-16 November 2000, Nashville, TN. Society of Environmental Toxicology and Chemistry, Pensacola, FL. Pp. 102.

Female mosquito fish (Gambusia...

37. Photograph of a line drawing. 'PART I, SECTION 8, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

37. Photograph of a line drawing. 'PART I, SECTION 8, BUILDINGS NO. G-1 TO G-10 INCL., PURIFICATION, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

52. Photograph of a line drawing. 'PART I, SECTION 8, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

52. Photograph of a line drawing. 'PART I, SECTION 8, BUILDINGS NO. 0-1, 0-3, 0-5, 0-7, 0-9, TESTING LABORATORY, MANUFACTURING AREA, PLANT B.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Space Maintenance and New Bone Formation with Polyurethane Biocomposites in a Canine Saddle Defect

DTIC Science & Technology

2014-05-01

Vanderbilt University, Nashville, TN 2. Medtronic Spinal and Biologics, Memphis, TN 3. US Army Institute of Surgical Research, Fort Sam Houston, TX...and 15% hydroxyapatite (HA) that is similar in mineral content to natural bone.3 45S5 Bioactive glass (BG) is a resorbable material that has been... used effectively in a variety of bone regeneration applications.4 In the present study, we investigated the ability of injectable PUR/MG and PUR/BG

ITS strategic deployment plan : Nashville area intelligent transportation systems early deployment study. Technical memorandum no. 8, Final report

DOT National Transportation Integrated Search

1997-03-01

The goal of this study was to develop a Strategic Plan for deployment of ITS technologies within the Nashville Metro Area and to create a long-term coalition of ITS stakeholders, with the objective of expanding the implementation of ITS technologies ...

PROCEEDINGS: SEVENTH SYMPOSIUM ON THE TRANSFER AND UTILIZATION OF PARTICULATE CONTROL TECHNOLOGY. VOLUME 1

EPA Science Inventory

The proceedings document presentations from the seventh symposium on the transfer and utilization of particulate control technology, March 22-25, 1988, in Nashville, TN. Objectives of the symposium were to encourage the exchange of new knowledge in the particulate control field b...

78 FR 64265 - Notice To Rescind a Notice of Intent To Prepare an Environmental Impact Statement (EIS): State...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-28

.... FOR FURTHER INFORMATION CONTACT: Ms. Theresa Claxton, Planning and Program Management Team Leader.... 615-781-5770. SUPPLEMENTARY INFORMATION: The FHWA, in cooperation with the Tennessee Department of... program.) Theresa Claxton, Planning and Program Mgmt. Team Leader, Nashville, TN. [FR Doc. 2013-25313...

75 FR 45660 - Notice of Inventory Completion: Tennessee Department of Environment and Conservation, Division of...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-03

... Department of Environment and Conservation, Division of Archaeology, Nashville, TN AGENCY: National Park... and associated funerary objects in the possession of the Tennessee Department of Environment and... assessment of the human remains was made by the Tennessee Department of Environment and Conservation...

Overview On Alternative Asbestos Control Method Research - Nashville, TN

EPA Science Inventory

The alternative asbestos control method (AACM) is an experimental approach to building demolition. Unlike the NESHAP method, the AACM allows some regulated asbestos-containing material to remain in the building and a surfactant-water solution is used to suppress asbestos fibers ...

42. Photograph of a line drawing. 'CROSS SECTION AND PLAN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

42. Photograph of a line drawing. 'CROSS SECTION AND PLAN LAYOUT OF PART I, SECTION 8, BUILDINGS NO. H-1 TO H-10 INCL., GRINDING, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (NashVille, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

29. Photograph of a line drawing. 'CROSS SECTION AND PLAN ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

29. Photograph of a line drawing. 'CROSS SECTION AND PLAN LAYOUT OF PART I, SECTION 8, BUILDINGS NO. D-1 TO D-10 INCL., NITRATION, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

76 FR 9812 - Notice Pursuant to the National Cooperative Research and Production Act of 1993-Marinenet, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2011-02-22

..., evaluating, testing and developing a process or service to establish and support a platform for the... invoking the Act's provisions limiting the recovery of antitrust plaintiffs to actual damages under... are: ACL Transportation Services LLC, Jeffersonville, IN; Ingram Barge Company, Nashville, TN; and AEP...

GONADAL DEVELOPMENT AND ENDOCRINE RESPONSES IN JAPANESE MEDAKA (ORYZIAS LATIPES) EXPOSED TO O.P'-DDT IN WATER OR THROUGH MATERNAL TRANSFER

EPA Science Inventory

In: Environmental Sciences in the 21st Century: Paradigms, Opportunities, and Challenges: Abstract Book: SETAC 21st Annual Meeting, 12-16 November 2000, Nashville, TN. Society of Environmental Toxicology and Chemistry, Pensacola, FL. Pp. p. 262. (ERL,GB R816).

Various i...

EMISSIONS OF BIOGENIC OXIDANT AND PM PRECURSORS- VERY HIGH REACTIVITY VOCS AND SURFACE LAYER CHEMISTRY ABOVE FORESTS

EPA Science Inventory

Recent analysis of ambient fine particulate matter (PM2.5) has found that significant portions of the organic matter contained therein are of biogenic origin. Radiocarbon (C-14) measurements of the bulk organic matter in fine particles collected near Nashville, TN, found that 40...

Airborne Passive Remote Sensing of the Troposphere in Nashville/Middle Tennessee Area During the 1995 Southern Oxidants Study

NASA Technical Reports Server (NTRS)

Rider, D. M.; Worden, H. M.; Beer, R.; Nandi, S.; Sparks, L. C.

1998-01-01

In July of 1995 the Airborne Emission Spectrometer was deployed to Nashville, Tennessee to participate in the 1995 Ozone Study Intensive Campaign of the Southern Oxidants Study. AES is a high resolution mid-infrared interferometer that measures the spectrum of upwelling radiation in the 650-4250 cm-1 range.

Williams communicates with the boys and girls at Middle Tennessee Nashville School during Expedition 13

NASA Image and Video Library

2006-08-17

ISS013-E-67441 (17 Aug. 2006) --- Astronaut Jeffrey N. Williams, Expedition 13 NASA space station science officer and flight engineer, holds a sleeping bag while conducting an educational teleconference with the Boys and Girls Clubs of Middle Tennessee in Nashville, via Ku- and S-band in the Destiny laboratory of the International Space Station, with audio and video relayed to the Mission Control Center at Johnson Space Center.

NOX/NOY MONITORING USING MODIFIED/UNMODIFIED COMMERCIAL INSTRUMENTATION

EPA Science Inventory

Three systems were used for monitoring oxides of nitrogen (NO, NO2, Noy, at the Cornelia Fort Airpark site in the 1999 Southern Oxidant Study near Nashville, TN during the months of June and July. Of the three systems, one was an unmodified ultraviolet differential optical abs...

78 FR 78474 - Knowledge Testing of New Entrant Motor Carriers, Freight Forwarders and Brokers

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-26

... regulations and industry practices for persons seeking registration authority as motor carriers (property... held at the Music City Center, 201 Fifth Ave. South, Nashville, TN 37203 in Room 202 C. In addition to... evidence of the individuals' knowledge of related rules, regulations, and industry practices.'' In...

RADIOCARBON 14C MEASUREMENTS ON ATMOSPHERIC SAMPLES OF PARTICULATE MATTER (& VOLATILE ORGANIC COMPOUNDS)

EPA Science Inventory

Following a brief history of radiocarbon work at EPA since the 1980's, the presentation focuses on recent non-winter measurements for PM-2.5 in the Southeastern U.S. (Houston, TX; Nashville, TN; and particularly, Tampa, FL) and what the measurements suggest about the importance o...

Tn552 transposase purification and in vitro activities.

PubMed Central

Rowland, S J; Sherratt, D J; Stark, W M; Boocock, M R

1995-01-01

The Staphylococcus aureus transposon Tn552 encodes a protein (p480) containing the 'D,D(35)E' motif common to retroviral integrases and the transposases of a number of bacterial elements, including phage Mu, the integron-containing element Tn5090, Tn7 and IS3. p480 and a histidine-tagged derivative were overexpressed in Escherichia coli and purified by methods involving denaturation and renaturation. DNase I footprinting and gel binding assays demonstrated that p480 binds to two adjacent, directly repeated 23 bp motifs at each end of Tn552. Although donor strand cleavage by p480 was not detected, in vitro conditions were defined for strand transfer activity with transposon end fragments having pre-cleaved 3' termini. Strand transfer was Mn(2+)-dependent and appeared to join a single left or right end fragment to target DNA. The importance of the terminal dinucleotide CA-3' was demonstrated by mutation. The in vitro activities of p480 are consistent with its proposed function as the Tn552 transposase. Images PMID:7828593

27. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

27. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS SECTION OF PART III, SECTION 1, EQUIPMENT LAYOUT, BUILDINGS C-1, C-3, C-5, C-6, C-7, C-9 INCL., MIXING, MANUFACTURING AREA, PLANT 'B'.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

75 FR 22435 - Pharos Capital Partners II, L.P.; Notice Seeking Exemption Under Section 312 of the Small...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

... SMALL BUSINESS ADMINISTRATION [License No. 06/76-0329] Pharos Capital Partners II, L.P.; Notice Seeking Exemption Under Section 312 of the Small Business Investment Act, Conflicts of Interest Notice is hereby given that Pharos Capital Partners II, L.P., 1 Burton Hills Boulevard, Suite 180, Nashville, TN...

76 FR 31632 - Notice of Receipt of Complaint; Solicitation of Comments Relating to the Public Interest

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-01

..., FL; AFC Trident Inc. of Chino, CA; Alibaba.com Hong Kong Ltd. of Hangzhou, China; Anbess Electronics Co. Ltd. of Shenzhen, China; Cellairis Franchise, Inc. of Alpharetta, GA; Cellet Products of Santa Fe Springs, CA; DHgate.com of Beijing, China; Griffin Technology, Inc. of Nashville, TN; Guangzhou Evotech...

Epidemiology of a thermonuclear bomb-burst over Nashville, Tennessee: a theoretic study.

PubMed

Quinn, R W

1983-07-01

A thermonuclear bomb explosion over any city in the world would have a devastating effect on the population and environment. For those who survive, with or without injuries, life would become primitive with little or no uncontaminated food or water, and with inadequate housing, fuel, and medical care, resulting in a breakdown of family and interpersonal relationships. This theoretic study of the potential outcome of a thermonuclear bomb-burst over Nashville, Tennessee, discusses epidemiologically the wide range of medical and psychologic effects from the direct trauma of blast and fire, widespread epidemics of otherwise controlled disease, long-term chronic illness, genetic damage, and catastrophic environmental havoc.

49. Photograph of a line drawing. 'PART I, SECTION 8, ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

49. Photograph of a line drawing. 'PART I, SECTION 8, BUILDINGS NO. K-1, K-3, K-5, K-7, K-9, K-10 OPENING, BUILDINGS NO. N-1 TO N-10 INCL., NAILING, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

26. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS ...

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

26. Photograph of a line drawing. 'PLAN LAYOUT AND CROSS SECTION OF PART I, SECTION 8, BUILDINGS NO. C-1, C-3, C-5, C-6, C-7, C-9, MIXING, MANUFACTURING AREA, PLANT B AS OF 4-24-44.' From the U.S. Army Corps of Engineers. Industrial Facilities Inventory, Holston Ordnance Works, Kingsport, Tennessee. Plant B, Parts II, III. (Nashville, TN: Office of the District Engineer, 1944). - Holston Army Ammunition Plant, RDX-and-Composition-B Manufacturing Line 9, Kingsport, Sullivan County, TN

Fast Track Randomized Controlled Trial to Prevent Externalizing Psychiatric Disorders: Findings from Grades 3 to 9

ERIC Educational Resources Information Center

Journal of the American Academy of Child & Adolescent Psychiatry, 2007

2007-01-01

Objective: This study tests the efficacy of the Fast Track Program in preventing antisocial behavior and psychiatric disorders among groups varying in initial risk. Method: Schools within four sites (Durham, NC; Nashville, TN; Seattle, WA; and rural central Pennsylvania) were selected as high-risk institutions based on neighborhood crime and…

Tn5401, a new class II transposable element from Bacillus thuringiensis.

PubMed Central

Baum, J A

1994-01-01

A new class II (Tn3-like) transposable element, designated Tn5401, was recovered from a sporulation-deficient variant of Bacillus thuringiensis subsp. morrisoni EG2158 following its insertion into a recombinant plasmid. Sequence analysis of the insert revealed a 4,837-bp transposon with two large open reading frames, in the same orientation, encoding proteins of 36 kDa (306 residues) and 116 kDa (1,005 residues) and 53-bp terminal inverted repeats. The deduced amino acid sequence for the 36-kDa protein shows 24% sequence identity with the TnpI recombinase of the B. thuringiensis transposon Tn4430, a member of the phage integrase family of site-specific recombinases. The deduced amino acid sequence for the 116-kDa protein shows 42% sequence identity with the transposase of Tn3 but only 28% identity with the TnpA transposase of Tn4430. Two small open reading frames of unknown function, designated orf1 (85 residues) and orf2 (74 residues), were also identified. Southern blot analysis indicated that Tn5401, in contrast to Tn4430, is not commonly found among different subspecies of B. thuringiensis and is not typically associated with known insecticidal crystal protein genes. Transposition was studied with B. thuringiensis by using plasmid pEG922, a temperature-sensitive shuttle vector containing Tn5401. Tn5401 transposed to both chromosomal and plasmid target sites but displayed an apparent preference for plasmid sites. Transposition was replicative and resulted in the generation of a 5-bp duplication at the target site. Transcriptional start sites within Tn5401 were mapped by primer extension analysis. Two promoters, designated PL and PR, direct the transcription of orf1-orf2 and tnpI-tnpA, respectively, and are negatively regulated by TnpI. Sequence comparison of the promoter regions of Tn5401 and Tn4430 suggests that the conserved sequence element ATGTCCRCTAAY mediates TnpI binding and cointegrate resolution. The same element is contained within the 53-bp terminal

Valor Collegiate Academies

ERIC Educational Resources Information Center

EDUCAUSE, 2015

2015-01-01

The four guiding principles behind the blended, competency-based, personalized learning model of Valor Collegiate Academies, a charter organization serving grades 5-12 in Nashville, TN: (1) Reflect the diversity of both our country and local community; (2) Personalize a student's experience to meet his/her unique academic and non-academic needs;…

Function of ZFAND3 in the DNA Damage Response

DTIC Science & Technology

2011-06-01

PRINCIPAL INVESTIGATOR: Bianca M. Sirbu CONTRACTING ORGANIZATION : Vanderbilt University...NUMBER E-Mail: bianca.m.sirbu@vanderbilt.edu 5f. WORK UNIT NUMBER 7. PERFORMING ORGANIZATION NAME(S) AND ADDRESS(ES) Vanderbilt University...Nashville, TN 37232 8. PERFORMING ORGANIZATION REPORT NUMBER 9. SPONSORING / MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10. SPONSOR/MONITOR’S

International Spread and Persistence of TEM-24 Is Caused by the Confluence of Highly Penetrating Enterobacteriaceae Clones and an IncA/C2 Plasmid Containing Tn1696::Tn1 and IS5075-Tn21▿

PubMed Central

Novais, Ângela; Baquero, Fernando; Machado, Elisabete; Cantón, Rafael; Peixe, Luísa; Coque, Teresa M.

2010-01-01

TEM-24 remains one of the most widespread TEM-type extended-spectrum β-lactamases (ESBLs) among Enterobacteriaceae. To analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative TEM-24 producers from Belgium, France, Portugal, and Spain (13 Enterobacter aerogenes isolates, 6 Escherichia coli isolates, 6 Klebsiella pneumoniae isolates, 2 Proteus mirabilis isolates, and 1 Klebsiella oxytoca isolate, from 1998 to 2004). Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] and E. coli phylogroups) and antibiotic susceptibility were determined by standard procedures. Plasmid analysis included determination of the incompatibility group (by PCR, hybridization, and/or sequencing) and comparison of restriction fragment length polymorphism (RFLP) patterns. Characterization of genetic elements conferring antibiotic resistance included integrons (classes 1, 2, and 3) and transposons (Tn3, Tn21, and Tn402). Similar PFGE patterns were identified among E. aerogenes, K. pneumoniae, and P. mirabilis isolates, while E. coli strains were diverse (phylogenetic groups A, B2, and D). Highly related 180-kb IncA/C2 plasmids conferring resistance to kanamycin, tobramycin, chloramphenicol, trimethoprim, and sulfonamides were identified. Each plasmid contained defective In0-Tn402 (dfrA1-aadA1, aacA4, or aacA4-aacC1-orfE-aadA2-cmlA1) and In4-Tn402 (aacA4 or dfrA1-aadA1) variants. These integrons were located within Tn21, Tn1696, or hybrids of these transposons, with IS5075 interrupting their IRtnp and IRmer. In all cases, blaTEM-24 was part of an IS5075-ΔTn1 transposon within tnp1696, mimicking other genetic elements containing blaTEM-2 and blaTEM-3 variants. The international dissemination of TEM-24 is fuelled by an IncA/C2 plasmid acquired by different enterobacterial clones which seem to evolve by gaining diverse genetic elements. This work highlights the risks of a confluence between highly

The Influence of High Computer Access on Schoolwork and Student Empowerment: An Exploratory Study of the Nashville ACOT Site.

ERIC Educational Resources Information Center

Fisher, Charles W.

This study examines the relationship between high computer access and "student empowerment" at the Nashville, Tennessee, site of the Apple Classroom of Tomorrow (ACOT) project. The study rests on the premise that school learning is a function of the work carried out by students in school, and that schoolwork is experienced by students as…

Epidemiology of a thermonuclear bomb-burst over Nashville, Tennessee: a theoretic study

DOE Office of Scientific and Technical Information (OSTI.GOV)

Quinn, R.W.

1983-07-01

A thermonuclear bomb explosion over any city in the world would have a devastating effect on the population and environment. For those who survive, with or without injuries, life would become primitive with little or no uncontaminated food or water, and with inadequate housing, fuel, and medical care, resulting in a breakdown of family and interpersonal relationships. This theoretic study of the potential outcome of a thermonuclear bomb-burst over Nashville, Tennessee, discusses epidemiologically the wide range of medical and psychologic effects from the direct trauma of blast and fire, widespread epidemics of otherwise controlled disease, long-term chronic illness, genetic damage,more » and catastrophic environmental havoc.« less

Building Bridges for Those under Bridges: A Lesson in Civility.

ERIC Educational Resources Information Center

Shields, Sharon L.; Sedille, Michael D.

1999-01-01

College students in a course, Health Service Delivery to Diverse Populations, examined the health-care needs and experiences of homeless people by surveying homeless people in Nashville, TN. This article presents the story of one homeless man and his transition off the streets. A photo essay chronicles his journey from the streets back into life…

Impact of a two-city community cancer prevention intervention on African Americans.

PubMed Central

Blumenthal, Daniel S.; Fort, Jane G.; Ahmed, Nasar U.; Semenya, Kofi A.; Schreiber, George B.; Perry, Shelley; Guillory, Joyce

2005-01-01

We report the first multisite, multicomponent community intervention trial to focus on cancer prevention in African Americans. The project explored the potential of historically black medical schools to deliver health information to their local communities and used a community-based participatory research approach. The intervention consisted of culturally sensitive messages at appropriate educational levels delivered over an 18-month period and tested in predominantly black census tracts in Nashville, TN and Atlanta, GA. Chattanooga, TN and Decatur, GA served as comparison cities. Results were evaluated by pre- and postintervention random-digit dial telephone surveys. The intervention cities showed an increase in reported contact with or knowledge of the project. There was little or no effect on knowledge or attitudes in the intervention cities. Compared to Chattanooga, Nashville showed an increase in percentage of women receiving Pap smears. Compared to Decatur, Atlanta showed an increase in percentage of age-appropriate populations receiving digital rectal exams, colorectal cancer screenings and mammograms. The results of this community intervention trial demonstrated modest success and are encouraging for future efforts of longer duration. PMID:16334495

Metropolitan Nashville's Innovation Zone: High-Need Schools Help Teacher-Leaders with Paid, Yearlong Student Teachers. An Opportunity Culture Case Study

ERIC Educational Resources Information Center

Barrett, Sharon Kebschul

2014-01-01

Better-prepared new teachers, more adults in every classroom, more small-group instruction, more adults caring for every student--how can a school wrap all that up in one package? Three Metropolitan Nashville Opportunity Culture schools are trying a novel approach with paid, yearlong student teaching positions. The three schools are: (1) Buena…

78 FR 40512 - Investigations Regarding Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-05

... (Company).... Chicago, IL 06/17/13 06/15/13 82815 Deloitte Services (State/ Hermitage, TN......... 06/18/13...). 82817 Volt (Apple Inc) (Workers). Austin, TX 06/18/13 06/11/13 82818 Propex Operating Company, Nashville, GA......... 06/18/13 06/17/13 LLC (Company). 82819 Vaughan Furniture Company Galax, VA 06/18/13 06/11...

Analytical evaluation of point of care cTnT and clinical performances in an unselected population as compared with central laboratory highly sensitive cTnT.

PubMed

Dupuy, Anne Marie; Sebbane, Mustapha; Roubille, François; Coste, Thibault; Bargnoux, Anne Sophie; Badiou, Stéphanie; Kuster, Nils; Cristol, Jean Paul

2015-03-01

To report the analytical performances of the Radiometer AQT90 FLEX® cTnT assay (Neuilly-Plaisance, France) and to evaluate the concordance with hs-cTnT results from central laboratory for the diagnosis of acute myocardial infarction (AMI) at baseline and during a short follow-up among unselected patients admitted in emergency room or cardiology department. Analytical performances of AQT90 FLEX® cTnT immunoassay included imprecision study with determination of a coefficient of variation at 10% and 20%, linearity, and limit of detection. The concordance study was based on samples obtained from 170 consecutive patients with chest pain suggestive of acute coronary syndrome (ACS) admitted in the emergency room or cardiology department. The kinetic study (within 62 additional samples 3h later) was based on absolute delta criterion and the combination of relative change of 30% with absolute change of 7ng/L. The cTnT assay from Radiometer was evaluated as clinically usable, although less sensitive than the Roche hs-cTnT assay as demonstrated by the concordance and the kinetic studies. In non-selected population, the cTnT AQT Flex© assay on AQT90© with kinetic change at 3h, provides similar clinical classification of patients, particularly for AMI group as compared to central laboratory hs-cTnT assay and could be suitable for clinical use. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Differential Treatment of Pregnancy in Employment: The Impact of "General Electric Co. v. Gilbert" and "Nashville Gas Co. v. Satty."

ERIC Educational Resources Information Center

Taylor, Ellen T.

1978-01-01

After discussing the facts and reasoning of the two cases (General Electric Co. vs Gilbert and Nashville Gas Co. vs Satty), the author argues that the decisions are largely the product of pregnancy stereotypes and that the Court's reasoning is flawed and should not be applied outside the context of pregnancy. Journal availability: see EA 511 481.…

Tn4556, a 6.8-kilobase-pair transposable element of Streptomyces fradiae.

PubMed Central

Chung, S T

1987-01-01

A 6.8-kilobase-pair (kbp) transposable element (Tn4556) was found in a neomycin-producing strain of Streptomyces fradiae. This element was first observed in two 30.3-kbp plasmids (pUC1123 and pUC1124) which arose when a thiostrepton resistance gene (1 kbp) was ligated with the BclI-2 fragment (22.5 kbp) that contains the origin of replication of phage SF1. The Tn4556 segment was deleted when these plasmids were transduced into another S. fradiae host with phage SF1. These deletion plasmids (pUC1210 and pUC1211) had copy numbers of less than 1 per chromosome and were unstable. In contrast, pUC1123 and pUC1124, with copy numbers of 12 to 15 per chromosome, respectively, were relatively stable. When pUC1210 and pUC1211 were reintroduced into S. fradiae by protoplast transformation, the Tn4556 element transposed again to the plasmids at numerous new locations in either of two orientations. A copy of Tn4556 was found in the S. fradiae chromosome by hybridization studies. It appears that Tn4556 originated from the chromosome, transposed into unstable pUC1210 and pUC1211, and made stable plasmids. A temperature-sensitive hybrid plasmid carrying a viomycin resistance derivative of Tn4556 (pMT660::Tn4556::vph) was constructed. When Streptomyces lividans UC8390 containing the hybrid plasmid was grown at 39 degrees C, Tn4556::vph (Tn4560) transposed to random positions in the host chromosome. Images PMID:2820925

Mini-Tn7 Insertion in Bacteria With Multiple glmS-Linked attTn7 Sites: Example Burkholderia Mallei ATCC 23344

DTIC Science & Technology

2006-06-27

INTRODUCTION Burkholderia mallei is the etiological agent of glanders and a highly evolved obligate zoonotic mammalian pathogen that naturally affects horses...mini-Tn7 insertion in bacteria with multiple glmS-linked attTn7 sites: example Burkholderia mallei ATCC 23344 Kyoung-Hee Choi1, David DeShazer2... glanders is a rare disease, B. mallei has received renewed attention because of its listing as a category B agent by the Centers for Disease Control

Occurrence and properties of composite transposon Tn2672: evolution of multiple drug resistance transposons.

PubMed Central

Hänni, C; Meyer, J; Iida, S; Arber, W

1982-01-01

We found Tn2671 (the 23-kb long IS1-flanked r-determinant of NR1-Basel) inserted into the ampicillin resistance gene bla of the Tn3-related transposon Tn902. The resulting 28-kilobase-long composite transposon Tn2672 (= Tn902 bla::Tn2671) is stable, and it translocates as a unit into various loci including IS1 of the resistance transfer factor of R100-1. These results are discussed with respect to the evolution of R plasmids providing multiple drug resistance. Images PMID:6281241

Tn5099, a xylE promoter probe transposon for Streptomyces spp.

PubMed Central

Hahn, D R; Solenberg, P J; Baltz, R H

1991-01-01

Tn5099, a promoter probe transposon for Streptomyces spp., was constructed by inserting a promoterless xylE gene and a hygromycin resistance gene into IS493. Tn5099 transposed into different sites in the Streptomyces griseofuscus genome, and the xylE reporter gene was expressed in some of the transposition mutants. Strains containing Tn5099 insertions that gave regulated expression of the xylE gene were identified. Images PMID:1653213

Fluxional Mechanocatalysis

DTIC Science & Technology

2015-01-29

Fl, Nov. 13, 2014. “Polymer Mechanochemistry: From Self -strengthening Rubbers to Soft Robots ”, Case Western Reserve University, Cleveland, OH...strengthening Rubbers and Soft Robot Camouflage”, Michelin Research Center, Clermont-Ferrand, France, July 3, 2014. “Mechanochemistry for Stress...Reactions to Adaptive Materials”, Department of Chemistry, Vanderbilt University, Nashville, TN, January 27, 2014. “From Rubber to Robots : the

Mississippi CaP HBCU Undergraduate Research Training Program

DTIC Science & Technology

2016-09-01

activities. This activity, occurred once a week (between weeks 4-6) and included touring to Urology, Hematology- Oncology , and Radiation Oncology facilities...Director of UMMC-Cancer Institute, Professor and Chairman, Department of Radiation Oncology University of Mississippi Medical Center, "Precision...Jackson, MS,4Vanderbilt University, Nashville, TN, 5Department of Pathology and Radiation Oncology , Mississippi Medical Center, Jackson, MS Tumor hypoxia

Assessment Tools for Basic Army Noncommissioned Officer Training

DTIC Science & Technology

2009-05-01

2008). Optimal learning in optimal contexts : The role of self -determination in education. Canadian Psychology, 49, 233–240. Magolda, M.B.B...1999). Creating contexts for learning and self -authorship: constructive- developmental pedagogy. Nashville, TN: Vanderbilt Press. Neisser, U...participation. New York, NY: Cambridge University Press. Magolda, M.B.B. (1999). Creating contexts for learning and self -authorship: constructive

An insight into the thermodynamic characteristics of human thrombopoietin complexation with TN1 antibody

PubMed Central

Shibazaki, Chie; Adachi, Motoyasu; Honjo, Eijiro; Tamada, Taro; Maeda, Yoshitake; Tahara, Tomoyuki; Kato, Takashi; Miyazaki, Hiroshi; Blaber, Michael; Kuroki, Ryota

2016-01-01

Abstract Human thrombopoietin (hTPO) primarily stimulates megakaryocytopoiesis and platelet production and is neutralized by the mouse TN1 antibody. The thermodynamic characteristics of TN1 antibody–hTPO complexation were analyzed by isothermal titration calorimetry (ITC) using an antigen‐binding fragment (Fab) derived from the TN1 antibody (TN1‐Fab). To clarify the mechanism by which hTPO is recognized by TN1‐Fab the conformation of free TN1‐Fab was determined to a resolution of 2.0 Å using X‐ray crystallography and compared with the hTPO‐bound form of TN1‐Fab determined by a previous study. This structural comparison revealed that the conformation of TN1‐Fab does not substantially change after hTPO binding and a set of 15 water molecules is released from the antigen‐binding site (paratope) of TN1‐Fab upon hTPO complexation. Interestingly, the heat capacity change (ΔCp) measured by ITC (−1.52 ± 0.05 kJ mol−1 K−1) differed significantly from calculations based upon the X‐ray structure data of the hTPO‐bound and unbound forms of TN1‐Fab (−1.02 ∼ 0.25 kJ mol−1 K−1) suggesting that hTPO undergoes an induced‐fit conformational change combined with significant desolvation upon TN1‐Fab binding. The results shed light on the structural biology associated with neutralizing antibody recognition. PMID:27419667

78 FR 72004 - Amendment of Class E Airspace; Tazewell, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-02

... Tazewell, TN, as new Standard Instrument Approach Procedures have been developed at New Tazewell Municipal Airport. This enhances the safety and management of aircraft operations at the airport. DATES: Effective..., TN. Airspace reconfiguration is necessary due to the development of the RNAV (GPS) RWY 7 approach and...

PD-1 suppresses development of humoral responses that protect against Tn-bearing tumors

PubMed Central

Haro, Marcela A.; Littrell, Chad A.; Yin, Zhaojun; Huang, Xuefei; Haas, Karen M.

2017-01-01

Tn is a carbohydrate antigen uniquely exposed on tumor mucins and thus, an ideal target for immunotherapy. However, it has been difficult to elicit protective antibody responses against Tn antigen and other tumor associated carbohydrate antigens. Our study demonstrates this can be attributed to PD-1 immuno-inhibition. Our data show a major role for PD-1 in suppressing mucin- and Tn-specific B-cell activation, expansion, and antibody production important for protection against Tn-bearing tumor cells. These Tn/mucin-specific B cells belong to the innate-like B-1b cell subset typically responsible for T cell–independent antibody responses. Interestingly, PD-1–mediated regulation is B cell–intrinsic and CD4+ cells play a key role in supporting Tn/mucin-specific B cell antibody production in the context of PD-1 deficiency. Mucin-reactive antibodies produced in the absence of PD-1 inhibition largely belong to the IgM subclass and elicit potent antitumor effects via a complement-dependent mechanism. The identification of this role for PD-1 in regulating B cell–dependent antitumor immunity to Tn antigen highlights an opportunity to develop new therapeutic strategies targeting tumor associated carbohydrate antigens. PMID:27856425

Characterization of Tn6238 with a New Allele of aac(6′)-Ib-cr

PubMed Central

Quiroga, María P.; Orman, Betina; Errecalde, Laura; Kaufman, Sara

2015-01-01

Here, we report that the genetic structure of Tn1331 remained conserved in Argentina from 1989 to 2013 (72 of 73 isolates), with the exception being the plasmid-borne Tn1331-like transposon Tn6238 containing a new aac(6′)-Ib-cr allele recovered from a colistin-resistant Klebsiella pneumoniae clinical isolate. A bioinformatic analysis of aac(6′)-Ib-like gene cassettes suggests that this new aac(6′)-Ib-cr allele emerged through mutation or homologous recombination in the Tn1331 genetic platform. Tn6238 is a novel platform for the dissemination of aminoglycoside and fluoroquinolone resistance determinants. PMID:25691640

Long-term tillage and cropping effects on biological properties associated with soil aggregation in semi-arid eastern Montana, USA

USDA-ARS?s Scientific Manuscript database

Long-term tillage and cropping may influence biological attributes responsible for semi-arid soil aggregation in Montana, USA. Aggregate stability, glomalin, basidiomycete fungi, uronic acids, total organic C (TOC) and total N (TN) at 0-5 cm soil depth from 1991 to 2003 were evaluated in different a...

RADIOCARBON MEASUREMENTS ON PM 2.5 AMBIENT AEROSOL FROM NASHVILLE, TN

EPA Science Inventory

Radiocarbon (Carbon-14) measurements provide an estimate of the fraction of carbon in a sample that is biogenic. The methodology has been extensively used in past wintertime studies to quantify the contribution of wood smoke to ambient aerosol. In summertime such measurements...

Registration of TN09-008 soybean cyst nematode resistant cultivar

USDA-ARS?s Scientific Manuscript database

The conventional soybean line TN09-008 (Reg. No. CV- , PI ) was released by University of Tennessee Agricultural Research in 2017 as a cultivar, based on high seed yield potential in Tennessee and the southern region. Soybean cultivar TN09-008 is resistant to HG types 1.2.5.7, 5.7, a...

Myofilament Calcium Sensitivity: Mechanistic Insight into TnI Ser-23/24 and Ser-150 Phosphorylation Integration

PubMed Central

Salhi, Hussam E.; Hassel, Nathan C.; Siddiqui, Jalal K.; Brundage, Elizabeth A.; Ziolo, Mark T.; Janssen, Paul M. L.; Davis, Jonathan P.; Biesiadecki, Brandon J.

2016-01-01

Troponin I (TnI) is a major regulator of cardiac muscle contraction and relaxation. During physiological and pathological stress, TnI is differentially phosphorylated at multiple residues through different signaling pathways to match cardiac function to demand. The combination of these TnI phosphorylations can exhibit an expected or unexpected functional integration, whereby the function of two phosphorylations are different than that predicted from the combined function of each individual phosphorylation alone. We have shown that TnI Ser-23/24 and Ser-150 phosphorylation exhibit functional integration and are simultaneously increased in response to cardiac stress. In the current study, we investigated the functional integration of TnI Ser-23/24 and Ser-150 to alter cardiac contraction. We hypothesized that Ser-23/24 and Ser-150 phosphorylation each utilize distinct molecular mechanisms to alter the TnI binding affinity within the thin filament. Mathematical modeling predicts that Ser-23/24 and Ser-150 phosphorylation affect different TnI affinities within the thin filament to distinctly alter the Ca2+-binding properties of troponin. Protein binding experiments validate this assertion by demonstrating pseudo-phosphorylated Ser-150 decreases the affinity of isolated TnI for actin, whereas Ser-23/24 pseudo-phosphorylation is not different from unphosphorylated. Thus, our data supports that TnI Ser-23/24 affects TnI-TnC binding, while Ser-150 phosphorylation alters TnI-actin binding. By measuring force development in troponin-exchanged skinned myocytes, we demonstrate that the Ca2+ sensitivity of force is directly related to the amount of phosphate present on TnI. Furthermore, we demonstrate that Ser-150 pseudo-phosphorylation blunts Ser-23/24-mediated decreased Ca2+-sensitive force development whether on the same or different TnI molecule. Therefore, TnI phosphorylations can integrate across troponins along the myofilament. These data demonstrate that TnI Ser-23

Proteolytic Processing of Laminin-332 by Hepsin and Matriptase and Its Role in Prostate Cancer Progression

DTIC Science & Technology

2011-09-01

epithelial tumors, including breast, cervix , esophagus, liver, mesothelium, prostate, and colorectal cancers [36,38,61–69]. Interestingly, in the case of...Proteolytic Processing of Laminin-332 by Hepsin and Matriptase and Its Role in Prostate Cancer Progression Manisha Tripathi The Vanderbilt University...Nashville, TN 37203 Laminin-332 is lost in prostate cancer progression. Laminin-332 is known to be cleaved by various cell surface proteases

Genetic and DNA sequence analysis of the kanamycin resistance transposon Tn903.

PubMed Central

Grindley, N D; Joyce, C M

1980-01-01

The kanamycin resistance transposon Tn903 consists of a unique region of about 1000 base pairs bounded by a pair of 1050-base-pair inverted repeat sequences. Each repeat contains two Pvu II endonuclease cleavage sites separated by 520 base pairs. We have constructed derivatives of Tn903 in which this 520-base-pair fragment is deleted from one or both repeats. Those derivatives that lack both 520-base-pair fragments cannot transpose, whereas those that lack just one remain transposition proficient. One such transposable derivative, Tn903 delta I, has been selected for further study. We have determined the sequence of the intact inverted repeat. The 18 base pairs at each end are identical and inverted relative to one another, a structure characteristic of insertion sequences. Additional experiments indicate that a single inverted repeat from Tn903 can, in fact, transpose; we propose that this element be called IS903. To correlate the DNA sequence with genetic activities, we have created mutations by inserting a 10-base-pair DNA fragment at several sites within the intact repeat of Tn903 delta 1, and we have examined the effect of such insertions on transposability. The results suggest that IS903 encodes a 307-amino-acid polypeptide (a "transposase") that is absolutely required for transposition of IS903 or Tn903. Images PMID:6261245

75 FR 20774 - Establishment of Class E Airspace; Mountain City, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... DEPARTMENT OF TRANSPORTATION Federal Aviation Administration 14 CFR Part 71 [Docket No. FAA-2009-0061; Airspace Docket No. 09-ASO-10] Establishment of Class E Airspace; Mountain City, TN AGENCY... December 7, 2009 that establishes Class E airspace at Johnson County Airport, Mountain City, TN. DATES...

United States Air Force Graduate Student Research Program for 1990. Program Technical Report. Volume 1

DTIC Science & Technology

1991-06-05

functions on the excavator. 28-6 m0 E Figure 1: Conceptual User Interface for the Rapid Runway Repair (RRR) Remote Control System IV. COMMUNICATION SYSTEM...Vehicle Systems Conference, Dayton, OH. Mariani, D., 1988, "Robotic Vehicle Communications Interoperability," RD& E Center Technical Report, US Army Tank...D.g e : BS Va;,derbilt University Specialty: Computer Engineering Electrical Eng. Dept. Absigned: Arnold Er-gineering Nashville, TN 37240 Developmer

Treatment of Early Post-op Wound Infection after Internal Fixation

DTIC Science & Technology

2015-10-01

Obremskey, M.D. CONTRACTING ORGANIZATION: Vanderbilt University Medical Center Nashville TN 37203 REPORT DATE: October 2015 TYPE OF REPORT: Annual...PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. 1. REPORT DATE (DD-MM-YYYY) October 2015 2. REPORT TYPE Annual 3. DATES COVERED (From - To...either: (Group 1) operative debridement and PO antibiotic treatment for 6 weeks; or (Group 2) operative debridement and IV antibiotics for 6 weeks and

Operation TOMODACHI: A Model for American Disaster Response Efforts and the Collective use of Military Forces Abroad

DTIC Science & Technology

2012-01-01

Plant in Fukushima Daiichi (approximately 170 miles North of Tokyo). The plant consisted of six nuclear reactors and a series of spent-fuel pools...should be praised for the decision to allow family members to voluntarily evacuate areas within 200 miles of the Fukushima - Daiichi Nuclear Plant... Disaster ” (power point presentation, Airlift Tanker Association, Nashville, TN, November 4, 2011) 3 Hisaya Sugiyama, “AIA Summary of Fukushima

Nuclear Matrix Proteins in Disparity of Prostate Cancer

DTIC Science & Technology

2010-07-01

Arlington, VA 22202- 4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty... VA Medical Center7, New Orleans, LA, and Vanderbilt University School of Medicine8, Nashville, TN. (** see attached abstract) 3. Manuscript...grant # PC081594). Citation Format: Yang Y, Jia D, Davis R, Moparty K, Thomas R, Moroz K, Agrawal K, Abdel- Mageed A. Heterogeneous nuclear

Individual Differences in Planning-Related Activities for Simple Digital Circuit Design

DTIC Science & Technology

1994-01-01

Learning Technology Center Vanderbilt University 94-1 Box 45, Peabody Nashville, TN 37203 g. SPONSORING/ MONITORING AGENCY NAME(S) AND ADDRESS(ES) 10...they learned in class, rather than first constructing a deeper understanding of what role each component played in the problem-solving process. One of...Pezdek, & W. P. Banks (Eds.), Application of cognitive psychology: Problen, suiving, education and computing (pp. 123-137). Hillsdale, NJ: Erlbaum. Chase

Integration of Genomic, Biologic, and Chemical Approaches to Target p53 Loss and Gain-of-Function in Triple Negative Breast Cancer

DTIC Science & Technology

2015-09-01

dissertation research is determining the mechanism and “targetability” of a mutant p53-adapted state in triple-negative breast cancer . Tim’s...Negative Breast Cancer PRINCIPAL INVESTIGATOR: Jennifer A. Pietenpol, Ph.D. CONTRACTING ORGANIZATION: The Vanderbilt University Nashville, TN...Loss and Gain-of-Function in Triple Negative Breast Cancer 5a. CONTRACT NUMBER W81XWH-13-1-0287 p53 Loss and Gain-of-Function in Triple Negative

The diagnostic value of two commercially available human cTnI assays in goat kids with myocarditis.

PubMed

Karapinar, Tolga; Eroksuz, Yesari; Hayirli, Armagan; Beytut, Enver; Kaynar, Ozgur; Baydar, Ersoy; Sozdutmaz, Ibrahim; Isidan, Hakan

2016-03-01

Cardiac troponin I (cTnI) is a peripheral blood marker for myocardial damage. Because of the unavailability of goat-specific cTnI assays human cTnI assays may be validated for detection of myocarditis in goat kids. The purpose of the study was to evaluate 2 commercially available human cTnI assays in goat kids with myocardial damage, and to determine the cTnI expression in cardiac muscle. Plasma cTnI concentrations were measured in healthy goat kids (n = 7) and goat kids with myocardial damage (n = 8) using the Beckman Coulter Access Accu TnI and the Biomérieux Vidas Ultra. The results were correlated with gross necropsy and histopathologic findings, and cTnI immunhistochemistry in cardiac tissue. Macro- and microscopic findings confirmed myocardial damage in the myocarditis group. Mean plasma cTnI concentration was significantly higher in the myocarditis group than in the healthy control group (104.82 vs 0.02 ng/mL). The overall mean plasma cTnI concentration measured by Biomérieux Vidas Ultra (61.75 ng/mL, 95% CI: 19.55-103.95) was comparable to the mean measured by Beckman Coulter Access Accu TnI (50.08 ng/mL, 95% CI: 24.11-76.06), and cTnI concentrations measured by these assays were highly correlated (r = .977) with a -6.2% bias. Both assays were precise and accurate. The human-specific Beckman Coulter Access Accu TnI and the Biomérieux Vidas Ultra can be used for diagnostic confirmation of myocardial damage in caprine medicine. © 2016 American Society for Veterinary Clinical Pathology.

The Levels and Distribution of TN, TP and TOC in the South China Sea

NASA Astrophysics Data System (ADS)

Wang, H.; Han, D.

2012-04-01

The marine biogeochemistries of carbon, nitrogen and phosphorous have come under increased scrutiny because of their close involvement in climate change and coastal eutrophication. The South China Sea is unique in that located in a subtropical zone, and therefore represents an important regime for biogeochemical studies. However, to our knowledge, few data are available for total nitrogen (TN), total phosphorous (TP) and total organic carbon (TOC) in South Sea, China. The present study aims to contribute to the knowledge of their status through investigating the level and distribution of TN, TP and TOC in South China Sea. A total of 108 seawater samples of 11 sites in south sea, china were collected during August 29- September 4, 2006. An automated and simultaneous method for determination of TN and TOC was applied to all seawater samples. The combined system allowed simultaneous determination for TOC and TN in the same sample using a single injection and provided low detection limits and excellent linear ranges for both TOC and TN. The risk of contamination has been remarkably reduced due to the minimal sample manipulation and automated analyses. And quantitative analyses of TP in seawater were accomplished by a typical chemical method. Concentration ranges of TN and TP were 0.06-0.67, and 0.003-0.071 mg/L, respectively, as well as that of TOC were 0.23-2.51mg/L. The values of TN and TP showed that the status of nutrition is relatively better in south china sea than other marine areas. Moreover, the upright change trend of TN concentration level as well as TP and TOC according to the experimental results at the total 11 sites are simultaneity studied. The concentration of TN initial increases with the increasing of the depth, later the value becomes almost constant. In contrast, the concentration of TOC reduces with the increasing of the depth, later the value becomes almost unchangeable. Compared with the trend of TN and TOC, that of TP appears relatively stable

Transposon mutagenesis of Xylella fastidiosa by electroporation of Tn5 synaptic complexes.

PubMed

Guilhabert, M R; Hoffman, L M; Mills, D A; Kirkpatrick, B C

2001-06-01

Pierce's disease, a lethal disease of grapevine, is caused by Xylella fastidiosa, a gram-negative, xylem-limited bacterium that is transmitted from plant to plant by xylem-feeding insects. Strains of X. fastidiosa also have been associated with diseases that cause tremendous losses in many other economically important plants, including citrus. Although the complete genome sequence of X. fastidiosa has recently been determined, the inability to transform or produce transposon mutants of X. fastidiosa has been a major impediment to understanding pathogen-, plant-, and insect-vector interactions. We evaluated the ability of four different suicide vectors carrying either Tn5 or Tn10 transposons as well as a preformed Tn5 transposase-transposon synaptic complex (transposome) to transpose X. fastidiosa. The four suicide vectors failed to produce any detectable transposition events. Electroporation of transposomes, however, yielded 6 x 10(3) and 4 x 10(3) Tn5 mutants per microg of DNA in two different grapevine strains of X. fastidiosa. Molecular analysis showed that the transposition insertions were single, independent, stable events. Sequence analysis of the Tn5 insertion sites indicated that the transpositions occur randomly in the X. fastidiosa genome. Transposome-mediated mutagenesis should facilitate the identification of X. fastidiosa genes that mediate plant pathogenicity and insect transmission.

RadNet Air Data From Knoxville, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Knoxville, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

RadNet Air Data From Memphis, TN

EPA Pesticide Factsheets

This page presents radiation air monitoring and air filter analysis data for Memphis, TN from EPA's RadNet system. RadNet is a nationwide network of monitoring stations that measure radiation in air, drinking water and precipitation.

cTnT can be a useful marker for early detection of anthracycline cardiotoxicity.

PubMed

Kilickap, S; Barista, I; Akgul, E; Aytemir, K; Aksoyek, S; Aksoy, S; Celik, I; Kes, S; Tekuzman, G

2005-05-01

The level of serum cardiac troponin-T (cTnT) increases with myocardial damage. We sought to assess whether cTnT level could be a useful marker for the early detection of anthracycline cardiotoxicity. Forty-one patients who had been scheduled to receive anthracycline-containing combination chemotherapy were included in the study. Serum cTnT levels were measured before (baseline) and after the first cycle of chemotherapy, and again, after the last cycle of chemotherapy. In all patients, the left ventricular ejection fraction (LVEF), fractional shortening (FS), early peak flow/atrial flow velocity (E/A) ratio, and the isovolemic relaxation time (IRT) were measured echocardiographically, both before and after the completion of chemotherapy. LVEF and FS did not change in any patients. In 21 patients (49%), the E/A ratio decreased after therapy as compared to the pre-treatment values. The decrease in E/A ratio was more prominent in patients who were older than the mean age of our study group, which was 44 years. The post-treatment IRT was prolonged compared with the pretreatment IRT (94.0 +/- 2.0 versus 85.6 +/- 10.5 ms, respectively). cTnT levels after completion of therapy were elevated in 14 (34%) patients, and exceeded the upper limit of the normal range (>0.1 ng/ml) in only one patient. cTnT levels measured after completion of therapy were significantly higher, compared with those measured at baseline and after the first cycle of therapy. In the younger age group (< or =44 years old), there was a two-fold decrease in the E/A ratio in those patients whose cTnT levels increased during the therapy, when compared with those whose cTnT levels did not change (21% versus 43%, respectively). Increased serum cTnT level can be detected in the early stages of anthracycline therapy and it is associated with diastolic dysfunction of the left ventricle. Therefore, serum cTnT level could be a useful measure for early detection of anthracycline-induced cardiotoxicity.

Iceland's Grímsvötn volcano erupts

NASA Astrophysics Data System (ADS)

Showstack, Randy

2011-05-01

About 13 months after Iceland's Eyjafjallajökull volcano began erupting on 14 April 2010, which led to extensive air traffic closures over Europe, Grímsvötn volcano in southeastern took its turn. Iceland's most active volcano, which last erupted in 2004 and lies largely beneath the Vatnajökull ice cap, began its eruption activity on 21 May, with the ash plume initially reaching about 20 kilometers in altitude, according to the Icelandic Meteorological Office. Volcanic ash from Grímsvötn has cancelled hundreds of airplane flights and prompted U.S. president Barack Obama to cut short his visit to Ireland. As Eos went to press, activity at the volcano was beginning to subside.

Iceland: Grímsvötn Volcano

Atmospheric Science Data Center

2013-04-17

article title:  Grímsvötn Volcano Injects Ash into the Stratosphere     ... p.m. local time (1730 UTC) on Saturday, May 21, 2011. The volcano, located approximately 140 miles (220 kilometers) east of the capital ...

The novel conjugative transposon tn1207.3 carries the macrolide efflux gene mef(A) in Streptococcus pyogenes.

PubMed

Santagati, Maria; Iannelli, Francesco; Cascone, Carmela; Campanile, Floriana; Oggioni, Marco R; Stefani, Stefania; Pozzi, Gianni

2003-01-01

The macrolide efflux gene mef(A) of the Streptococcus pyogenes clinical strain 2812A was found to be carried by a 52-kb chromosomal genetic element that could be transferred by conjugation to the chromosome of other streptococcal species. The characteristics of this genetic element are typical of conjugative transposons and was named Tn1207.3. The size of Tn1207.3 was established by pulsed-field gel electrophoresis (PFGE), and DNA sequencing analysis showed that the 7,244 bp at the left end of Tn1207.3 were identical to those of the pneumococcal Tn1207.1 element. Tn1207.3-like genetic elements were found to be inserted at a single specific chromosomal site in 12 different clinical isolates S. pyogenes exhibiting the M phenotype of resistance to macrolides and carrying the mef(A) gene. Tn1207.3 was transferred from S. pyogenes 2812A to Streptococcus pneumoniae, and sequence analysis carried out on six independent transconjugants showed that insertion of Tn1207.3 in the pneumococcal genome always occurred at a single specific site as in Tn1207.1. Using MF2, a representative S. pneumoniae transconjugant, as a donor, Tn1207.3 was transferred again by conjugation to S. pyogenes and Streptococcus gordonii. The previously described nonconjugative element Tn1207.1 of S. pneumoniae appears to be a defective element, part of a longer conjugative transposon that carries mef(A) and is found in clinical isolates of S. pyogenes.

The Tn7 transposition regulator TnsC interacts with the transposase subunit TnsB and target selector TnsD

PubMed Central

Choi, Ki Young; Spencer, Jeanelle M.; Craig, Nancy L.

2014-01-01

The excision of transposon Tn7 from a donor site and its insertion into its preferred target site, attachment site attTn7, is mediated by four Tn7-encoded transposition proteins: TnsA, TnsB, TnsC, and TnsD. Transposition requires the assembly of a nucleoprotein complex containing all four Tns proteins and the DNA substrates, the donor site containing Tn7, and the preferred target site attTn7. TnsA and TnsB together form the heteromeric Tn7 transposase, and TnsD is a target-selecting protein that binds specifically to attTn7. TnsC is the key regulator of transposition, interacting with both the TnsAB transposase and TnsD-attTn7. We show here that TnsC interacts directly with TnsB, and identify the specific region of TnsC involved in the TnsB–TnsC interaction during transposition. We also show that a TnsC mutant defective in interaction with TnsB is defective for Tn7 transposition both in vitro and in vivo. Tn7 displays cis-acting target immunity, which blocks Tn7 insertion into a target DNA that already contains Tn7. We provide evidence that the direct TnsB–TnsC interaction that we have identified also mediates cis-acting Tn7 target immunity. We also show that TnsC interacts directly with the target selector protein TnsD. PMID:24982178

Overview On Alternate Asbestos Control Method Research And NESHAP Comparison - Nashville, TN

EPA Science Inventory

The alternative asbestos control method (AACM) is an experimental approach to building demolition. Unlike the NESHAP method, the AACM allows some regulated asbestos containing material to remain in the building and a surfactant-water solution is used to suppress asbestos fibers ...

Large-Scale Low-Cost NGS Library Preparation Using a Robust Tn5 Purification and Tagmentation Protocol

PubMed Central

Hennig, Bianca P.; Velten, Lars; Racke, Ines; Tu, Chelsea Szu; Thoms, Matthias; Rybin, Vladimir; Besir, Hüseyin; Remans, Kim; Steinmetz, Lars M.

2017-01-01

Efficient preparation of high-quality sequencing libraries that well represent the biological sample is a key step for using next-generation sequencing in research. Tn5 enables fast, robust, and highly efficient processing of limited input material while scaling to the parallel processing of hundreds of samples. Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His6-Sumo3 tag. We demonstrate that libraries prepared with our in-house Tn5 are of the same quality as those processed with a commercially available kit (Nextera XT), while they dramatically reduce the cost of large-scale experiments. We introduce improved purification strategies for two versions of the Tn5 enzyme. The first version carries the previously reported point mutations E54K and L372P, and stably produces libraries of constant fragment size distribution, even if the Tn5-to-input molecule ratio varies. The second Tn5 construct carries an additional point mutation (R27S) in the DNA-binding domain. This construct allows for adjustment of the fragment size distribution based on enzyme concentration during tagmentation, a feature that opens new opportunities for use of Tn5 in customized experimental designs. We demonstrate the versatility of our Tn5 enzymes in different experimental settings, including a novel single-cell polyadenylation site mapping protocol as well as ultralow input DNA sequencing. PMID:29118030

Brookside Mills, Knox County, TN

EPA Pesticide Factsheets

Brookside Mills, located in Knox County, TN, was a textile mill that was founded in 1885 and at its peak employed over 1,000 people. Its former uses included fabric weaving, dying, and sewing operations. It was at some point a department store, and during a portion of its history, coal was used as an energy source. Weaving operations continued in some form at the Brookside factory until 1969. In 1996 the buildings were demolished.

76 FR 18288 - Tennessee Disaster #TN-00048

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-01

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12497 and 12498] Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an... this disaster for physical damage is 12497 6 and for economic injury is 12498 0. The State which...

78 FR 48762 - Tennessee Disaster #TN-00076

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-09

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13696 and 13697] Tennessee Disaster TN-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 08/02/2013. Incident: Severe Storms and...

75 FR 55833 - Tennessee Disaster #TN-00042

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-14

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12303 and 12304] Tennessee Disaster TN-00042 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of Tennessee dated 09/07/2010. Incident: Severe Storms and...

77 FR 51100 - Tennessee Disaster #TN-00068

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-23

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 13215 and 13216] Tennessee Disaster TN-00068 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of TENNESSEE dated. 08/16/2012. Incident: Severe storms...

76 FR 20433 - Tennessee Disaster #TN-00048

Federal Register 2010, 2011, 2012, 2013, 2014

2011-04-12

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12497 and 12498] Tennessee Disaster TN-00048 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is a an amendment of the Administrative declaration of a disaster for the State of Tennessee dated 03/23/2011. Incident: Severe Storms and...

Two-stage soil infiltration treatment system for treating ammonium wastewaters of low COD/TN ratios.

PubMed

Lei, Zhongfang; Wu, Ting; Zhang, Yi; Liu, Xiang; Wan, Chunli; Lee, Duu-Jong; Tay, Joo-Hwa

2013-01-01

Soil infiltration treatment (SIT) is ineffective to treat ammonium wastewaters of total nitrogen (TN) > 100 mg l(-1). This study applied a novel two-stage SIT process for effective TN removal from wastewaters of TN>100 mg l(-1) and of chemical oxygen demand (COD)/TN ratio of 3.2-8.6. The wastewater was first fed into the soil column (stage 1) at hydraulic loading rate (HLR) of 0.06 m(3) m(-2) d(-1) for COD removal and total phosphorus (TP) immobilization. Then the effluent from stage 1 was fed individually into four soil columns (stage 2) at 0.02 m(3) m(-2) d(-1) of HLR with different proportions of raw wastewater as additional carbon source. Over the one-year field test, balanced nitrification and denitrification in the two-stage SIT revealed excellent TN removal (>90%) from the tested wastewaters. Copyright © 2012 Elsevier Ltd. All rights reserved.

Christian Theological Attitudes vis-a-vis Islam: The Effect on West-Muslim Relations

DTIC Science & Technology

2002-12-01

through the Bible , which communicates God’s eternal purpose in the person of Jesus Christ. The Scriptures, writings made infallible by the directing of...Holy Bible (Nashville, TN: Thomas Nelson Publishers, 1982). See Book of the Psalms 19:1-6, a central passage to the Christian defense of God’s general...revelation and the Epistle of Paul the Apostle to the Romans 18:18-21. 20 The Holy Bible , Philippians 2:6-11. 10 This passage from the Apostle

An Enterobacter plasmid as a new genetic background for the transposon Tn1331

PubMed Central

Alavi, Mohammad R; Antonic, Vlado; Ravizee, Adrien; Weina, Peter J; Izadjoo, Mina; Stojadinovic, Alexander

2011-01-01

Background Genus Enterobacter includes important opportunistic nosocomial pathogens that could infect complex wounds. The presence of antibiotic resistance genes in these microorganisms represents a challenging clinical problem in the treatment of these wounds. In the authors’ screening of antibiotic-resistant bacteria from complex wounds, an Enterobacter species was isolated that harbors antibiotic-resistant plasmids conferring resistance to Escherichia coli. The aim of this study was to identify the resistance genes carried by one of these plasmids. Methods The plasmids from the Enterobacter isolate were propagated in E. coli and one of the plasmids, designated as pR23, was sequenced by the Sanger method using fluorescent dyeterminator chemistry on a genetic analyzer. The assembled sequence was annotated by search of the GenBank database. Results Plasmid pR23 is composed of the transposon Tn1331 and a backbone plasmid that is identical to the plasmid pPIGDM1 from Enterobacter agglomerans. The multidrug-resistance transposon Tn1331, which confers resistance to aminoglycoside and beta lactam antibiotics, has been previously isolated only from Klebsiella. The Enterobacter plasmid pPIGDM1, which carries a ColE1-like origin of replication and has no apparent selective marker, appears to provide a backbone for propagation of Tn1331 in Enterobacter. The recognition sequence of Tn1331 transposase for insertion into pPIGDM1 is the pentanucleotide TATTA, which occurs only once throughout the length of this plasmid. Conclusion Transposition of Tn1331 into the Enterobacter plasmid pPIGDM1 enables this transposon to propagate in this Enterobacter. Since Tn1331 was previously isolated only from Klebsiella, this report suggests horizontal transfer of this transposon between the two bacterial genera. PMID:22259249

Development of a community-sensitive strategy to increase availability of fresh fruits and vegetables in Nashville's urban food deserts, 2010-2012.

PubMed

Larson, Celia; Haushalter, Alisa; Buck, Tracy; Campbell, David; Henderson, Trevor; Schlundt, David

2013-07-25

Food deserts, areas that lack full-service grocery stores, may contribute to rising rates of obesity and chronic diseases among low-income and racial/ethnic minority residents. Our corner store project, part of the Centers for Disease Control and Prevention's Communities Putting Prevention to Work initiative, aimed to increase availability of healthful foods in food deserts in Nashville, Tennessee. We identified 4 food deserts in which most residents are low-income and racially and ethnically diverse. Our objectives were to develop an approach to increase availability of fresh fruits and vegetables, low-fat or nonfat milk, and 100% whole-wheat bread in Nashville's food deserts and to engage community members to inform our strategy. Five corner stores located in food deserts met inclusion criteria for our intervention. We then conducted community listening sessions, proprietor surveys, store audits, and customer-intercept surveys to identify needs, challenges to retailing the products, and potential intervention strategies. Few stores offered fresh fruits, fresh vegetables, low-fat or nonfat milk, or 100% whole-wheat bread, and none stocked items from all 4 categories. Major barriers to retailing healthful options identified by community members are mistrust of store owners, history of poor-quality produce, and limited familiarity with healthful options. Store owners identified neighborhood crime as the major barrier. We used community input to develop strategies. Engaging community residents and understanding neighborhood context is critical to developing strategies that increase access to healthful foods in corner stores.

Effects of HCM cTnI Mutation R145G on Troponin Structure and Modulation by PKA Phosphorylation Elucidated by Molecular Dynamics Simulations

PubMed Central

Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J. Andrew

2015-01-01

Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI1-39), is a target for phosphorylation by protein kinase A (PKA) during β-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl137–147) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca2+ handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, cTnI-R145G/S23D/S24D Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, and cTnI-R145G/PS23/PS24 Ca2+-bound cTnC1-161-cTnI1-172-cTnT236-285, respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca2+-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca2+ coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N-terminal extension and the cTnI inhibitory

Effects of HCM cTnI mutation R145G on troponin structure and modulation by PKA phosphorylation elucidated by molecular dynamics simulations.

PubMed

Lindert, Steffen; Cheng, Yuanhua; Kekenes-Huskey, Peter; Regnier, Michael; McCammon, J Andrew

2015-01-20

Cardiac troponin (cTn) is a key molecule in the regulation of human cardiac muscle contraction. The N-terminal cardiac-specific peptide of the inhibitory subunit of troponin, cTnI (cTnI(1-39)), is a target for phosphorylation by protein kinase A (PKA) during β-adrenergic stimulation. We recently presented evidence indicating that this peptide interacts with the inhibitory peptide (cTnl(137-147)) when S23 and S24 are phosphorylated. The inhibitory peptide is also the target of the point mutation cTnI-R145G, which is associated with hypertrophic cardiomyopathy (HCM), a disease associated with sudden death in apparently healthy young adults. It has been shown that both phosphorylation and this mutation alter the cTnC-cTnI (C-I) interaction, which plays a crucial role in modulating contractile activation. However, little is known about the molecular-level events underlying this modulation. Here, we computationally investigated the effects of the cTnI-R145G mutation on the dynamics of cTn, cTnC Ca(2+) handling, and the C-I interaction. Comparisons were made with the cTnI-R145G/S23D/S24D phosphomimic mutation, which has been used both experimentally and computationally to study the cTnI N-terminal specific effects of PKA phosphorylation. Additional comparisons between the phosphomimic mutations and the real phosphorylations were made. For this purpose, we ran triplicate 150 ns molecular dynamics simulations of cTnI-R145G Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), cTnI-R145G/S23D/S24D Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), and cTnI-R145G/PS23/PS24 Ca(2+)-bound cTnC(1-161)-cTnI(1-172)-cTnT(236-285), respectively. We found that the cTnI-R145G mutation did not impact the overall dynamics of cTn, but stabilized crucial Ca(2+)-coordinating interactions. However, the phosphomimic mutations increased overall cTn fluctuations and destabilized Ca(2+) coordination. Interestingly, cTnI-R145G blunted the intrasubunit interactions between the cTnI N

76 FR 36166 - Tennessee Disaster Number TN-00051

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-21

... U.S. SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12556 and 12557] Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 4. SUMMARY: This is an... loan applications to: U.S. Small Business Administration, Processing and Disbursement Center, 14925...

76 FR 48939 - Tennessee Disaster Number TN-00058

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-09

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12704 and 12705] Tennessee Disaster Number TN-00058 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of... information in the original declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers...

75 FR 35103 - Tennessee Disaster Number TN-00038

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-21

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12161 and 12162] Tennessee Disaster Number TN-00038 AGENCY: Small Business Administration. ACTION: Amendment 8. SUMMARY: This is an amendment of the... the original declaration remains unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and...

75 FR 35103 - Tennessee Disaster Number TN-00039

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-21

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12159 and 12160] Tennessee Disaster Number TN-00039 AGENCY: Small Business Administration. ACTION: Amendment 7. SUMMARY: This is an amendment of the... Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster...

Spatiotemporal patterns of infant bronchiolitis in a Tennessee Medicaid population.

PubMed

Sloan, Chantel D; Gebretsadik, Tebeb; Wu, Pingsheng; Carroll, Kecia N; Mitchel, Edward F; Hartert, Tina V

2013-09-01

Respiratory syncytial virus (RSV) is a major cause of worldwide morbidity and mortality in infants, primarily through the induction of bronchiolitis. RSV epidemics are highly seasonal, occurring in the winter months in the northern hemisphere. Within the United States, RSV epidemic dynamics vary both spatially and temporally. This analysis employs a retrospective space–time scan statistic to locate spatiotemporal clustering of infant bronchiolitis in a very large Tennessee (TN) Medicaid cohort. We studied infants less than 6 months of age (N = 52,468 infants) who had an outpatient visit, emergency department visit, or hospitalization for bronchiolitis between 1995 and 2008. The scan statistic revealed distinctive and consistent patterns of deviation in epidemic timing. Eastern TN (Knoxville area) showed clustering in January and February, and Central TN (Nashville area) in November and December. This is likely due to local variation in geography-associated factors which should be taken into consideration in future modeling of RSV epidemics.

76 FR 27739 - Tennessee Disaster Number TN-00051

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-12

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12556 and 12557] Tennessee Disaster Number TN-00051 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of... Assistance Numbers 59002 and 59008) James E. Rivera, Associate Administrator for Disaster Assistance. [FR Doc...

76 FR 32387 - Tennessee Disaster Number TN-00053

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-06

... SMALL BUSINESS ADMINISTRATION [Disaster Declaration 12572 and 12573] Tennessee Disaster Number TN-00053 AGENCY: U.S. Small Business Administration. ACTION: Amendment 1. SUMMARY: This is an amendment of... unchanged. (Catalog of Federal Domestic Assistance Numbers 59002 and 59008) James E. Rivera, Associate...

Role of Myofibril-Inducing RNA in cardiac TnT expression in developing Mexican axolotl

PubMed Central

Sferrazza, Gian-Franco; Zhang, Chi; Jia, Pingping; Lemanski, Sharon L.; Athauda, Gagani; Stassi, Alyssa; Halager, Kristine; Maier, Jennifer A.; Rueda-de-Leon, Elena; Gupta, Amit; Dube, Syamalima; Huang, Xupei; Prentice, Howard M.; Dube, Dipak K.; Lemanski, Larry F.

2007-01-01

The Mexican axolotl, Ambystoma mexicanum, has been a useful animal model to study heart development and cardiac myofibrillogenesis. A naturally-occurring recessive mutant, gene “c”, for cardiac non-function in the Mexican axolotl causes a failure of myofibrillogenesis due to a lack of tropomyosin expression in homozygous mutant (c/c) embryonic hearts.. Myofibril-Inducing RNA (MIR) rescues mutant hearts in vitro by promoting tropomyosin expression and myofibril formation thereafter. We have studied the effect of MIR on the expression of various isoforms of cardiac Troponin-T (cTnT), a component of the thin filament that binds with tropomyosin. Four alternatively spliced cTnT isoforms have been characterized from developing axolotl heart. The expression of various cTnT isoforms in normal, mutant, and mutant hearts corrected with MIR, is evaluated by real-time RT-PCR using isoform specific primer pairs; MIR affects the total transcription as well as the splicing of the cTnT in axolotl heart PMID:17408593

Review of Environmental Consequences of Waterway Design and Construction Practices as of 1979.

DTIC Science & Technology

1982-04-01

AD-A118 07Th VANDERISILT UNIV NASHVILLE I TN F/ ,2 REVIEW OF ENVIRONMENTAL CONSEQUENCES OF WATERWAY DESIGN AND CON-ETC(U U CLASSIFIED APR 82 E L... DESIGN AND CONSTRUCTION PRACTICES AS OF 1979 8. PERFORMING ORG. REPORT NUMEER 7. AUTHOR(q) S. CONTRACT OR GRANT NUMUER(t) Edward L. Thackston...numF*-" a I~mOlPi lt block ninr) >. Waterway projects designed and constructed by the Corps of Engineers (CE) include dikes, revetments, levees, and

The Next Generation: Access and Opportunity in Continuing Higher Education. Proceedings of the Annual Meeting of the Association for Continuing Higher Education (70th, Nashville, Tennessee, November 8-11, 2008)

ERIC Educational Resources Information Center

Barrineau, Irene T., Ed.

2008-01-01

These proceedings record the 70th Annual Conference and Meeting of ACHE (Association for Continuing Higher Education) held at the Gaylord Opryland Resort and Convention Center in Nashville, Tennessee. The conference theme, "The Next Generation: Access and Opportunity in CHE" challenged attendees to think about the coming new age and its…

Response of high-risk of recurrence/progression bladder tumours expressing sialyl-Tn and sialyl-6-T to BCG immunotherapy

PubMed Central

Lima, L; Severino, P F; Silva, M; Miranda, A; Tavares, A; Pereira, S; Fernandes, E; Cruz, R; Amaro, T; Reis, C A; Dall'Olio, F; Amado, F; Videira, P A; Santos, L; Ferreira, J A

2013-01-01

Background: High risk of recurrence/progression bladder tumours is treated with Bacillus Calmette-Guérin (BCG) immunotherapy after complete resection of the tumour. Approximately 75% of these tumours express the uncommon carbohydrate antigen sialyl-Tn (Tn), a surrogate biomarker of tumour aggressiveness. Such changes in the glycosylation of cell-surface proteins influence tumour microenvironment and immune responses that may modulate treatment outcome and the course of disease. The aim of this work is to determine the efficiency of BCG immunotherapy against tumours expressing sTn and sTn-related antigen sialyl-6-T (s6T). Methods: In a retrospective design, 94 tumours from patients treated with BCG were screened for sTn and s6T expression. In vitro studies were conducted to determine the interaction of BCG with high-grade bladder cancer cell line overexpressing sTn. Results: From the 94 cases evaluated, 36 had recurrence after BCG treatment (38.3%). Treatment outcome was influenced by age over 65 years (HR=2.668; (1.344–5.254); P=0.005), maintenance schedule (HR=0.480; (0.246–0.936); P=0.031) and multifocallity (HR=2.065; (1.033–4.126); P=0.040). sTn or s6T expression was associated with BCG response (P=0.024; P<0.0001) and with increased recurrence-free survival (P=0.001). Multivariate analyses showed that sTn and/or s6T were independent predictive markers of recurrence after BCG immunotherapy (HR=0.296; (0.148–0.594); P=0.001). In vitro studies demonstrated higher adhesion and internalisation of the bacillus to cells expressing sTn, promoting cell death. Conclusion: s6T is described for the first time in bladder tumours. Our data strongly suggest that BCG immunotherapy is efficient against sTn- and s6T-positive tumours. Furthermore, sTn and s6T expression are independent predictive markers of BCG treatment response and may be useful in the identification of patients who could benefit more from this immunotherapy. PMID:24064971

Expression of sialosyl-Tn in colony-forming unit-erythroid, erythroblasts, B cells, and a subset of CD4+ cells.

PubMed

Muroi, K; Suda, T; Nakamura, M; Okada, S; Nojiri, H; Amemiya, Y; Miura, Y; Hakomori, S

1994-01-01

The epitopes Tn and sialosyl-Tn are expressed on erythrocytes of individuals with a very rare blood group, who often suffer from "Tn syndrome." We surveyed expression of Tn and sialosyl-Tn in normal blood cells, malignant transformed cells, and progenitor stem cells from bone marrow (BM). An anti-Tn antibody, IE3, and an anti-sialosyl-Tn antibody, TKH2, were used in this study. TKH2 reacted with erythroblasts, B cells, and a subset of CD4+ cells; but not with erythrocytes. Erythroblastic cell lines (K562, HEL, and UT7/EPO) and B-cell lines (Daudi, Raji, and B-cell lines transformed by Epstein-Barr virus) showed reactivity to TKH2. Similar results from the reactivity of TKH2 with transformed cells from leukemia patients and lymphoma patients were obtained; TKH2 reacted with blasts from erythroleukemia (M6; for 4 of 4 cases) and with lymphocytes from B-cell chronic lymphocytic leukemia (3 of 3), B-cell lymphoma (5 of 5), and CD4+ adult T-cell leukemia (4 of 4), but did not react with blasts from acute myeloid leukemia (M0 to M5; 0 of 22) or acute lymphoid leukemia (B-lymphoid leukemia, 0 of 11; T-lymphoid leukemia, 0 of 2; undifferentiated leukemia, 0 of 1). IE3 did not react with all of the tested cells. CD2-CD19-TKH2+ normal BM cells (BMC) contained blasts and various maturation stages of erythroblasts. The TKH2+ cells produced a large number of colony-forming unit-erythroid (CFU-E) colonies, whereas they produced a small number of burst-forming unit-erythroid colonies and CFU-granulocyte-macrophage colonies. CD34+ normal BMC did not express Tn and sialosyl-Tn. These findings suggest that sialosyl-Tn expresses in CFU-E to erythroblasts.

A new frontier: applying comprehensive DM strategies to healthy members.

PubMed

2001-02-01

Extend disease management services to 'healthy' members. Why? Because with all the emphasis on high-cost, chronically ill patients, a health plan's most valuable asset is being ignored. At least that's the case being made by Nashville, TN-based American Healthways, which has rolled out a new program designed to put health plans in touch with all their members, not just the chronically ill. Through the use of predictive modeling, the program is designed to target higher risk members so preventive strategies can be employed.

Archaeological Reconnaissance Survey and Salvage Excavation in the Salt Lick Recreation Area.

DTIC Science & Technology

1979-04-01

ANTHROPOLOGY D 8 BALL UNCLASSIFIED APR 79 DACN62-76-N-3194 F/G 5/6 NL lllEEllEElllEhhhE|hh|h|hEE Emmhhhmhhhhml IIIIIEE/I/BBBK EmihgmhEhhhEI *81uu11uuur1u... Anthropology Knoxville, TN I I. CONTROLLING OFFICE NAME AND ADDRESS 12. REPORT DATE U. S. Army Engineer District, Nashville April, 1979 P.O. Box 1070 13. NUMBER...are stored in the Department of Anthropology , University of k Tennessee, Knoxville. A SECURITY CLAFIATI S I ARCHAEOLOGICAL RECONNAISSANCE, SURVEY AND

Julian Davies and the discovery of kanamycin resistance transposon Tn5.

PubMed

Berg, Douglas E

2017-04-01

This paper recounts some of my fond memories of a collaboration between Julian Davies and myself that started in 1974 in Geneva and that led to our serendipitous discovery of the bacterial kanamycin resistance transposon Tn5, and aspects of the lasting positive impact of our interaction and discovery on me and the community. Tn5 was one of the first antibiotic resistance transposons to be found. Its analysis over the ensuing decades provided valuable insights into mechanisms and control of transposition, and led to its use as a much-valued tool in diverse areas of molecular genetics, as also will be discussed here.

76 FR 4147 - Putnam-Cumberland, TN-Improve Power Supply

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-24

... TENNESSEE VALLEY AUTHORITY Putnam-Cumberland, TN--Improve Power Supply AGENCY: Tennessee Valley... proposed electrical power supply improvements in the Putnam and Cumberland region of east-central Tennessee... supplies bulk electric power to Cumberland and Putnam counties and the immediately surrounding areas in...

Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

NASA Astrophysics Data System (ADS)

Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

2016-09-01

The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between -35 and -10 elements.

76 FR 35909 - Temporary Concession Contract for Big South Fork National Recreation Area, TN/KY

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... Recreation Area, TN/KY. SUMMARY: Pursuant to 36 CFR 51.24, public notice is hereby given that the National... Concession Contract for Big South Fork National Recreation Area, TN/KY AGENCY: National Park Service... services within Big South Fork National Recreation Area, Tennessee and Kentucky, for a term not to exceed 3...

77 FR 13195 - Amendment of Class E Airspace; Springfield, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-06

..., Georgia 30320; telephone (404) 305-6364. SUPPLEMENTARY INFORMATION: History On September 22, 2011, the FAA... areas extending upward from 700 feet or more above the surface of the earth. * * * * * ASO TN E5...

76 FR 1512 - Amendment of Class E Airspace; Savannah, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-11

..., Georgia 30320; telephone (404) 305-5610. SUPPLEMENTARY INFORMATION: History On October 26, 2010, the FAA... areas extending upward from 700 feet or more above the surface of the earth. * * * * * ASO TN E5...

78 FR 55770 - [Tennessee Disaster greek-iTN-00077

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-11

... SMALL BUSINESS ADMINISTRATION Disaster Declaration 13737 and 13738 [Tennessee Disaster TN-00077] AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a notice of an Administrative declaration of a disaster for the State of TENNESSEE dated 08/26/2013. Incident: Severe Storms and...

A critical evaluation of the Beckman Coulter Access hsTnI: Analytical performance, reference interval and concordance.

PubMed

Pretorius, Carel J; Tate, Jillian R; Wilgen, Urs; Cullen, Louise; Ungerer, Jacobus P J

2018-05-01

We investigated the analytical performance, outlier rate, carryover and reference interval of the Beckman Coulter Access hsTnI in detail and compared it with historical and other commercial assays. We compared the imprecision, detection capability, analytical sensitivity, outlier rate and carryover against two previous Access AccuTnI assay versions. We established the reference interval with stored samples from a previous study and compared the concordances and variances with the Access AccuTnI+3 as well as with two commercial assays. The Access hsTnI had excellent analytical sensitivity with the calibration slope 5.6 times steeper than the Access AccuTnI+3. The detection capability was markedly improved with the SD of the blank 0.18-0.20 ng/L, LoB 0.29-0.33 ng/L and LoD 0.58-0.69 ng/L. All the reference interval samples had a result above the LoB value. At a mean concentration of 2.83 ng/L the SD was 0.28 ng/L (CV 9.8%). Carryover (0.005%) and outlier (0.046%) rates were similar to the Access AccuTnI+3. The combined male and female 99th percentile reference interval was 18.2 ng/L (90% CI 13.2-21.1 ng/L). Concordance amongst the assays was poor with only 16.7%, 19.6% and 15.2% of samples identified by all 4 assays as above the 99th, 97.5th and 95th percentiles. Analytical imprecision was a minor contributor to the observed variances between assays. The Beckman Coulter Access hsTnI assay has excellent analytical sensitivity and precision characteristics close to zero. This allows cTnI measurement in all healthy individuals and the capability to identify numerically small differences between serial samples as statistically significant. Concordance in healthy individuals remains poor amongst assays. Crown Copyright © 2018. Published by Elsevier Inc. All rights reserved.

High-sensitive cardiac troponin hs-TnT levels in sudden deaths related to atherosclerotic coronary artery disease.

PubMed

Beausire, Tim; Faouzi, Mohamed; Palmiere, Cristian; Fracasso, Tony; Michaud, Katarzyna

2018-06-04

Ischemic heart disease (IHD) related to atherosclerotic coronary artery disease (CAD) is one of the most prevalent causes of death in Europe. Postmortem evaluation of IHD remains a challenge because of possible non-specific autopsy finding in some autopsy cases, especially in early myocardial ischemia. High-sensitive cardiac troponin T (hs-TnT) is used today in clinical practice as the "gold standard" to diagnose the myocardial ischemia, and might also be applied as an ancillary tool for post-mortem evaluation. The goal of this study is to evaluate the diagnostic value of post-mortem serum hs-TnT assay in cases of sudden death related to IHD. We will also investigate the influence of cardiopulmonary resuscitation (CPR) attempts on post-mortem hs-TnT levels. The hs-TnT values in serum were retrospectively analysed in 85 autopsy data. 52 cases with clinical history and morphological results suggesting cardiac ischemia were included in the study group (mean age 53.5; age range 34-75) and 33 cases in the control group (mean age 40.4; age range 15-69). The group's statistical comparison was performed using logistic regression model. Our study showed a significant non-linear association between hs-TnT serum values and post-mortem diagnosis of sudden deaths related to IHD (p-value 0.005). The shape of the relationship is showing that the probability of death due to IHD increases quickly with a light level of hs-TnT (maximum around 90ng/L) then decreases slightly while remaining at high in values. No significant difference in the hs-TnT serum values was found between the CPR and the non-CPR cases (p-value 0.304). The measurement of hs-TnT serum values might be considered as an ancillary tool for the evaluation of death related to IHD, while taking necessary precautions in the interpretation of the results. Copyright © 2018 Elsevier B.V. All rights reserved.

Experimental manipulation of TN:TP ratiossuppress cyanobacterial biovolume and microcystinconcentration in large-scale in situ mesocosms

USGS Publications Warehouse

Harris, Theodore D.; Wilhelm, Frank M.; Graham, Jennifer L.; Loftin, Keith A.

2014-01-01

A global dataset was compiled to examine relations between the total nitrogen to total phosphorus ratio (TN:TP) and microcystin concentration in lakes and reservoirs. Microcystin concentration decreased as TN:TP ratios increased, suggesting that manipulation of the TN:TP ratio may reduce microcystin concentrations. This relationship was experimentally tested by adding ammonium nitrate to increase the TN:TP ratio in large-scale (70 m3), in situ mesocosms located in a eutrophic reservoir that routinely experiences toxic blooms of cyanobacteria. At a TN:TP ratio >75:1, chlorophytes dominated the phytoplankton community in the mesocosms, while cyanobacterial biovolume was significantly reduced and microcystin was not detected. In contrast, the unmanipulated reservoir was dominated by cyanobacteria, and microcystin was detected. Secchi depths were 1.1 to 1.8 times greater in the mesocosms relative to the reservoir. Cladoceran zooplankton had a larger body size (0.14 mm on average) in the mesocosms compared to conspecifics in the reservoir, which was likely related to the higher quality food. Combined, these empirical and experimental data indicate that although nutrient addition is counterintuitive to current cyanobacteria management practices, increasing the TN:TP ratio by adding nitrogen may be a potential short-term management strategy to reduce cyanobacteria and cyanotoxins when other alternatives (e.g., phosphorus reduction) are not possible. Additional experimental studies with careful controls are needed to define best management practices and identify any potential unintended consequences before nitrogen addition is implemented as a lake and reservoir management practice.

Recent increases in sediment and nutrient accumulation in Bear Lake, Utah/Idaho, USA

USGS Publications Warehouse

Smoak, J.M.; Swarzenski, P.W.

2004-01-01

This study examines historical changes in sediment and nutrient accumulation rates in Bear Lake along the northeastern Utah/Idaho border, USA. Two sediment cores were dated by measuring excess 210Pb activities and applying the constant rate of supply (CRS) dating model. Historical rates of bulk sediment accumulation were calculated based on the ages within the sediment cores. Bulk sediment accumulation rates increased throughout the last 100 years. According to the CRS model, bulk sediment accumulation rates were <25mg cm-2 year-1 prior to 1935. Between 1935 and 1980, bulk sediment accumulation rates increased to approximately 40mg cm -2 year-1. This increase in sediment accumulation probably resulted from the re-connection of Bear River to Bear Lake. Bulk sediment accumulation rates accelerated again after 1980. Accumulation rates of total phosphorus (TP), total nitrogen (TN), total inorganic carbon (TIC), and total organic carbon (TOC) were calculated by multiplying bulk sediment accumulation rates times the concentrations of these nutrients in the sediment. Accumulation rates of TP, TN, TIC, and TOC increased as a consequence of increased bulk sediment accumulation rates after the re-connection of Bear River with Bear Lake.

Structural Analysis of the Hg(II)-Regulatory Protein Tn501 MerR from Pseudomonas aeruginosa

PubMed Central

Wang, Dan; Huang, Shanqing; Liu, Pingying; Liu, Xichun; He, Yafeng; Chen, Weizhong; Hu, Qingyuan; Wei, Tianbiao; Gan, Jianhua; Ma, Jing; Chen, Hao

2016-01-01

The metalloprotein MerR is a mercury(II)-dependent transcriptional repressor-activator that responds to mercury(II) with extraordinary sensitivity and selectivity. It’s widely distributed in both Gram-negative and Gram-positive bacteria but with barely detectable sequence identities between the two sources. To provide structural basis for the considerable biochemical and biophysical experiments previously performed on Tn501 and Tn21 MerR from Gram-negative bacteria, we analyzed the crystal structure of mercury(II)-bound Tn501 MerR. The structure in the metal-binding domain provides Tn501 MerR with a high affinity for mercury(II) and the ability to distinguish mercury(II) from other metals with its unique planar trigonal coordination geometry, which is adopted by both Gram-negative and Gram-positive bacteria. The mercury(II) coordination state in the C-terminal metal-binding domain is transmitted through the allosteric network across the dimer interface to the N-terminal DNA-binding domain. Together with the previous mutagenesis analyses, the present data indicate that the residues in the allosteric pathway have a central role in maintaining the functions of Tn501 MerR. In addition, the complex structure exhibits significant differences in tertiary and quaternary structural arrangements compared to those of Bacillus MerR from Gram-positive bacteria, which probably enable them to function with specific promoter DNA with different spacers between −35 and −10 elements. PMID:27641146

Transponson Tn916 Mutagenesis in Bacillus anthracis,

DTIC Science & Technology

1987-11-10

Tngla, is described. Tng1a was transferred from Streptococcus 1aJaji strain DS16C1 to f. a VNR-1 by conjugation in a standard filter mating procedure...transposon, Tn916, mutagenesis, Bacillus, anthracis, subtilis. , Streptococcus , faecalis, aro 2.AUSrN ACT (Cautious no reverse efho if nece.at7r sd ideratfy...transferred from Streptococcus : faecalis strain DS16CI to B. anthracis VNR-1 by conjugation in a standard filter mating procedure. Tetracycline

The fully synthetic MAG-Tn3 therapeutic vaccine containing the tetanus toxoid-derived TT830-844 universal epitope provides anti-tumor immunity.

PubMed

Laubreton, Daphné; Bay, Sylvie; Sedlik, Christine; Artaud, Cécile; Ganneau, Christelle; Dériaud, Edith; Viel, Sophie; Puaux, Anne-Laure; Amigorena, Sebastian; Gérard, Catherine; Lo-Man, Richard; Leclerc, Claude

2016-03-01

Malignant transformations are often associated with aberrant glycosylation processes that lead to the expression of new carbohydrate antigens at the surface of tumor cells. Of these carbohydrate antigens, the Tn antigen is particularly highly expressed in many carcinomas, especially in breast carcinoma. We designed MAG-Tn3, a fully synthetic vaccine based on three consecutive Tn moieties that are O-linked to a CD4+ T cell epitope, to induce anti-Tn antibody responses that could be helpful for therapeutic vaccination against cancer. To ensure broad coverage within the human population, the tetanus toxoid-derived peptide TT830-844 was selected as a T-helper epitope because it can bind to various HLA-DRB molecules. We showed that the MAG-Tn3 vaccine, which was formulated with the GSK proprietary immunostimulant AS15 and designed for human cancer therapy, is able to induce an anti-Tn antibody response in mice of various H-2 haplotypes, and this response correlates with the ability to induce a specific T cell response against the TT830-844 peptide. The universality of the TT830-844 peptide was extended to new H-2 and HLA-DRB molecules that were capable of binding this T cell epitope. Finally, the MAG-Tn3 vaccine was able to induce anti-Tn antibody responses in cynomolgus monkeys, which targeted Tn-expressing tumor cells and mediated tumor cell death both in vitro and in vivo. Thus, MAG-Tn3 is a highly promising anticancer vaccine that is currently under evaluation in a phase I clinical trial.

Impacts of climate change on TN load and its control in a River Basin with complex pollution sources.

PubMed

Yang, Xiaoying; Warren, Rachel; He, Yi; Ye, Jinyin; Li, Qiaoling; Wang, Guoqing

2018-02-15

It is increasingly recognized that climate change could affect the quality of water through complex natural and anthropogenic mechanisms. Previous studies on climate change and water quality have mostly focused on assessing its impact on pollutant loads from agricultural runoff. A sub-daily SWAT model was developed to simulate the discharge, transport, and transformation of nitrogen from all known anthropogenic sources including industries, municipal sewage treatment plants, concentrated and scattered feedlot operations, rural households, and crop production in the Upper Huai River Basin. This is a highly polluted basin with total nitrogen (TN) concentrations frequently exceeding Class V of the Chinese Surface Water Quality Standard (GB3838-2002). Climate change projections produced by 16 Global Circulation Models (GCMs) under the RCP 4.5 and RCP 8.5 scenarios in the mid (2040-2060) and late (2070-2090) century were used to drive the SWAT model to evaluate the impacts of climate change on both the TN loads and the effectiveness of three water pollution control measures (reducing fertilizer use, constructing vegetative filter strips, and improving septic tank performance) in the basin. SWAT simulation results have indicated that climate change is likely to cause an increase in both monthly average and extreme TN loads in February, May, and November. The projected impact of climate change on TN loads in August is more varied between GCMs. In addition, climate change is projected to have a negative impact on the effectiveness of septic tanks in reducing TN loads, while its impacts on the other two measures are more uncertain. Despite the uncertainty, reducing fertilizer use remains the most effective measure for reducing TN loads under different climate change scenarios. Meanwhile, improving septic tank performance is relatively more effective in reducing annual TN loads, while constructing vegetative filter strips is more effective in reducing annual maximum monthly

Mobility and generation of mosaic non-autonomous transposons by Tn3-derived inverted-repeat miniature elements (TIMEs).

PubMed

Szuplewska, Magdalena; Ludwiczak, Marta; Lyzwa, Katarzyna; Czarnecki, Jakub; Bartosik, Dariusz

2014-01-01

Functional transposable elements (TEs) of several Pseudomonas spp. strains isolated from black shale ore of Lubin mine and from post-flotation tailings of Zelazny Most in Poland, were identified using a positive selection trap plasmid strategy. This approach led to the capture and characterization of (i) 13 insertion sequences from 5 IS families (IS3, IS5, ISL3, IS30 and IS1380), (ii) isoforms of two Tn3-family transposons--Tn5563a and Tn4662a (the latter contains a toxin-antitoxin system), as well as (iii) non-autonomous TEs of diverse structure, ranging in size from 262 to 3892 bp. The non-autonomous elements transposed into AT-rich DNA regions and generated 5- or 6-bp sequence duplications at the target site of transposition. Although these TEs lack a transposase gene, they contain homologous 38-bp-long terminal inverted repeat sequences (IRs), highly conserved in Tn5563a and many other Tn3-family transposons. The simplest elements of this type, designated TIMEs (Tn3 family-derived Inverted-repeat Miniature Elements) (262 bp), were identified within two natural plasmids (pZM1P1 and pLM8P2) of Pseudomonas spp. It was demonstrated that TIMEs are able to mobilize segments of plasmid DNA for transposition, which results in the generation of more complex non-autonomous elements, resembling IS-driven composite transposons in structure. Such transposon-like elements may contain different functional genetic modules in their core regions, including plasmid replication systems. Another non-autonomous element "captured" with a trap plasmid was a TIME derivative containing a predicted resolvase gene and a res site typical for many Tn3-family transposons. The identification of a portable site-specific recombination system is another intriguing example confirming the important role of non-autonomous TEs of the TIME family in shuffling genetic information in bacterial genomes. Transposition of such mosaic elements may have a significant impact on diversity and evolution, not

77 FR 13073 - Designation for the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-05

... the Jamestown, ND; Lincoln, NE; Memphis, TN; and Sioux City, IA Areas AGENCY: Grain Inspection..., IA areas, Lincoln, Midsouth, and Sioux City, respectively were the sole applicants for designation to.../2015 Midsouth Memphis, TN (901) 942-3216 4/1/2012 3/31/2015 Sioux City Sioux City, IA......... (712...

The effect and biological mechanism of COD/TN ratio on nitrogen removal in a novel upflow microaerobic sludge reactor treating manure-free piggery wastewater.

PubMed

Li, Jianzheng; Meng, Jia; Li, Jiuling; Wang, Cheng; Deng, Kaiwen; Sun, Kai; Buelna, Gerardo

2016-06-01

A novel upflow microaerobic sludge reactor (UMSR) was constructed to treat manure-free piggery wastewater with high NH4(+)-N concentration and low COD/TN ratio, and the effect and biological mechanism of COD/TN ratio on nitrogen removal were investigated at a constant hydraulic retention time of 8h and 35°C. The results showed that the UMSR could treat the wastewater with a better synchronous removal of COD, NH4(+)-N and TN. The microaerobic UMSR allowed nitrifiers, and heterotrophic and autotrophic denitrifiers to thrive in the flocs, revealing a multiple nitrogen removal mechanism in the reactor. Both the nitrifiers and denitrifiers would be restricted by an influent COD/TN ratio more than 0.82, resulting in a decrease of TN removal in the UMSR. To get a TN removal over 80% with a TN load removal above 0.86kg/(m(3)·d) in the UMSR, the influent COD/TN ratio should be less than 0.70. Copyright © 2016 Elsevier Ltd. All rights reserved.

Establishment of an Arbitrary PCR for Rapid Identification of Tn917 Insertion Sites in Staphylococcus epidermidis: Characterization of Biofilm-Negative and Nonmucoid Mutants

PubMed Central

Knobloch, Johannes K.-M.; Nedelmann, Max; Kiel, Kathrin; Bartscht, Katrin; Horstkotte, Matthias A.; Dobinsky, Sabine; Rohde, Holger; Mack, Dietrich

2003-01-01

Transposon mutagenesis with the Enterococcus faecalis transposon Tn917 is a genetic approach frequently used to identify genes related with specific phenotypes in gram-positive bacteria. We established an arbitrary PCR for the rapid and easy identification of Tn917 insertion sites in Staphylococcus epidermidis with six independent, well-characterized biofilm-negative Tn917 transposon mutants, which were clustered in the icaADBC gene locus or harbor Tn917 in the regulatory gene rsbU. For all six of these mutants, short chromosomal DNA fragments flanking both transposon ends could be amplified. All fragments were sufficient to correctly identify the Tn917 insertion sites in the published S. epidermidis genomes. By using this technique, the Tn917 insertion sites of three not-yet-characterized biofilm-negative or nonmucoid mutants were identified. In the biofilm-negative and nonmucoid mutant M12, Tn917 is inserted into a gene homologous to the regulatory gene purR of Bacillus subtilis and Staphylococcus aureus. The Tn917 insertions of the nonmucoid but biofilm-positive mutants M16 and M20 are located in genes homologous to components of the phosphoenolpyruvate-sugar phosphotransferase system (PTS) of B. subtilis, S. aureus, and Staphylococcus carnosus, indicating an influence of the PTS on the mucoid phenotype in S. epidermidis. PMID:14532029

Comparison of the gentamicin resistance transposon Tn5281 with regions encoding gentamicin resistance in Enterococcus faecalis isolates from diverse geographic locations.

PubMed Central

Hodel-Christian, S L; Murray, B E

1992-01-01

The genetic determinant encoding gentamicin resistance (Gmr) on the beta-lactamase encoding plasmid pBEM10 of Enterococcus faecalis HH22 is carried on a transposon, termed Tn5281, that is highly related to the staphylococcal Gmr transposons Tn4001 found in Australian isolates of Staphylococcus aureus and Tn4031 found in United States isolates of Staphylococcus epidermidis. We have now studied plasmid DNA from Gmr strains of E. faecalis isolated from diverse geographical locations (Houston, Pennsylvania, Thailand, and Chile) by using restriction endonuclease analysis and DNA-DNA hybridization to determine whether other Gmr E. faecalis carry Tn5281 or a similar type of element. We also compared these enterococci to several United States isolates of Staphylococcus aureus with nonmobile Gmr determinants. Three E. faecalis isolates (from Houston and Chile) carried Tn5281-like elements, whereas two isolates (from Houston and Pennsylvania) had restriction endonuclease and DNA-DNA hybridization patterns more similar to those of the Tn4001-IS257 hybrid found in the nonmobile Gmr determinants in United States isolates of S. aureus. A strain from Thailand had a third pattern unrelated to either Tn5281 or the nonmobile Gmr determinants present in United States isolates of S. aureus. Our results demonstrate that there is both similarity and diversity between the Gmr determinant of strains of E. faecalis isolated in diverse geographic locations. Images PMID:1332593

Optimization of high-rate TN removal in a novel constructed wetland integrated with microelectrolysis system treating high-strength digestate supernatant.

PubMed

Guo, Luchen; He, Keli; Wu, Shubiao; Sun, Hao; Wang, Yanfei; Huang, Xu; Dong, Renjie

2016-08-01

The potential of high-rate TN removal in three aerated horizontal subsurface-flow constructed wetlands to treat high-strength anaerobic digestate supernatant was evaluated. Different strategies of intermittent aeration and effluent recirculation were applied to compare their effect on nitrogen depuration performance. Additional glucose supply and iron-activated carbon based post-treatment systems were established and examined, respectively, to further remove nitrate that accumulated in the effluents from aerated wetlands. The results showed that intermittent aeration (1 h on:1 h off) significantly improved nitrification with ammonium removal efficiency of 90% (18.1 g/(m(2)·d)), but limited TN removal efficiency (53%). Even though effluent recirculation (a ratio of 1:1) increased TN removal from 53% to 71%, the effluent nitrate concentration was still high. Additional glucose was used as a post-treatment option and further increased the TN removal to 82%; however, this implementation caused additional organic pollution. Furthermore, the iron-activated carbon system stimulated with a microelectrolysis process achieved greater than 85% effluent nitrate removal and resulted in 86% TN removal. Considering the high TN removal rate, aerated constructed wetlands integrated with a microelectrolysis-driven system show great potential for treating high-strength digestate supernatant. Copyright © 2016 Elsevier Ltd. All rights reserved.

Comparison of cardiac TnI outliers using a contemporary and a high-sensitivity assay on the Abbott Architect platform.

PubMed

Ryan, J B; Southby, S J; Stuart, L A; Mackay, R; Florkowski, C M; George, P M

2014-07-01

Assays for cardiac troponin (cTn) have undergone improvements in sensitivity and precision in recent years. Increased rates of outliers, however, have been reported on various cTn platforms, typically giving irreproducible, falsely higher results. We aimed to evaluate the outlier rate occurring in patients with elevated cTnI using a contemporary and high-sensitivity assay. All patients with elevated cTnI (up to 300 ng/L) performed over a 21-month period were assayed in duplicate. A contemporary assay (Abbott STAT Troponin-I) was used for the first part of the study and subsequently a high-sensitivity assay (Abbott STAT High-Sensitive Troponin-I) was used. Outliers exceeded a calculated critical difference (CD) (CD = z × √2 × SDAnalytical) where z = 3.5 (for probability of 0.0005) and critical outliers also were on a different side of the decision level. The respective outlier and critical outlier rates were 0.22% and 0.10% for the contemporary assay (n = 4009) and 0.18% and 0.13% for the high-sensitivity assay (n = 3878). There was no significant reduction in outlier rate between the two assays (χ(2) = 0.034, P = 0.854). Fifty-six percent of outliers occurred in samples where cTn was an 'add-on' test (and was stored and refrigerated prior to assay). Despite recent improvements in cTn methods, outliers (including critical outliers) still occur at a low rate in both a contemporary and high-sensitivity cTnI assay. Laboratory and clinical staff should be aware of this potential analytical error, particularly in samples with suboptimal sample handling such as add-on tests. © The Author(s) 2014 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav.

Grímsvötn Volcano Injects Ash into the Stratosphere

NASA Image and Video Library

2011-05-24

NASA Terra spacecraft captured this image of Grímsvötn, the most active of Iceland volcanoes, which began erupting around 5:30 p.m. local time 1730 UTC on Saturday, May 21, 2011, east of the capital city of Reykjavik.

Hearing on the Rehabilitation, Education, and Training Programs Administered by the Veteran's Administration--Nashville, Tenn. Hearing before the Subcommittee on Education, Training, and Employment of the Committee on Veterans' Affairs. House of Representatives. Ninety-Sixty Congress, Second Session (September 26, 1980).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. House Committee on Veterans' Affairs.

These Congressional hearings contain testimony given in Nashville, Tennessee, concerning the administration, execution, and effectiveness of four rehabilitation, education, and training programs relating to veterans and their dependents that are administered by the Veteran's Administration. Programs reviewed are Vocational Rehabilitation; the G.I.…

Comparison of Friction Characteristics on TN and VA Mode Alignment Films with Friction Force Microscopy

NASA Astrophysics Data System (ADS)

Kwak, Musun; Chung, Hanrok; Kwon, Hyukmin; Kim, Jehyun; Han, Daekyung; Yi, Yoonseon; Lee, Sangmun; Lee, Chulgu; Cha, Sooyoul

Using frictional force microscopy (FFM), the friction surface characteristics were compared between twisted nematic (TN) mode and vertical alignment (VA) mode alignment films (AFs). The friction asymmetry was detected depending on temperature conditions on TN mode AF, but not on VA mode AF. The difference between two modes was explained by leaning intermolecular repulsion caused by the pre-tilt angle uniformity and the density of side chain. No level difference according to temperature conditions appeared when the pre-tilt angle were measured after liquid crystal (LC) injection.

Dynamic Equilibrium of Cardiac Troponin C's Hydrophobic Cleft and Its Modulation by Ca2+ Sensitizers and a Ca2+ Sensitivity Blunting Phosphomimic, cTnT(T204E).

PubMed

Schlecht, William; Dong, Wen-Ji

2017-10-18

Several studies have suggested that conformational dynamics are important in the regulation of thin filament activation in cardiac troponin C (cTnC); however, little direct evidence has been offered to support these claims. In this study, a dye homodimerization approach is developed and implemented that allows the determination of the dynamic equilibrium between open and closed conformations in cTnC's hydrophobic cleft. Modulation of this equilibrium by Ca 2+ , cardiac troponin I (cTnI), cardiac troponin T (cTnT), Ca 2+ -sensitizers, and a Ca 2+ -desensitizing phosphomimic of cTnT (cTnT(T204E) is characterized. Isolated cTnC contained a small open conformation population in the absence of Ca 2+ that increased significantly upon the addition of saturating levels of Ca 2+ . This suggests that the Ca 2+ -induced activation of thin filament arises from an increase in the probability of hydrophobic cleft opening. The inclusion of cTnI increased the population of open cTnC, and the inclusion of cTnT had the opposite effect. Samples containing Ca 2+ -desensitizing cTnT(T204E) showed a slight but insignificant decrease in open conformation probability compared to samples with cardiac troponin T, wild type [cTnT(wt)], while Ca 2+ sensitizer treated samples generally increased open conformation probability. These findings show that an equilibrium between the open and closed conformations of cTnC's hydrophobic cleft play a significant role in tuning the Ca 2+ sensitivity of the heart.

Correlating Calcium Binding, Förster Resonance Energy Transfer, and Conformational Change in the Biosensor TN-XXL

PubMed Central

Geiger, Anselm; Russo, Luigi; Gensch, Thomas; Thestrup, Thomas; Becker, Stefan; Hopfner, Karl-Peter; Griesinger, Christian; Witte, Gregor; Griesbeck, Oliver

2012-01-01

Genetically encoded calcium indicators have become instrumental in imaging signaling in complex tissues and neuronal circuits in vivo. Despite their importance, structure-function relationships of these sensors often remain largely uncharacterized due to their artificial and multimodular composition. Here, we describe a combination of protein engineering and kinetic, spectroscopic, and biophysical analysis of the Förster resonance energy transfer (FRET)-based calcium biosensor TN-XXL. Using fluorescence spectroscopy of engineered tyrosines, we show that two of the four calcium binding EF-hands dominate the FRET output of TN-XXL and that local conformational changes of these hands match the kinetics of FRET change. Using small-angle x-ray scattering and NMR spectroscopy, we show that TN-XXL changes from a flexible elongated to a rigid globular shape upon binding calcium, thus resulting in FRET signal output. Furthermore, we compare calcium titrations using fluorescence lifetime spectroscopy with the ratiometric approach and investigate potential non-FRET effects that may affect the fluorophores. Thus, our data characterize the biophysics of TN-XXL in detail and may form a basis for further rational engineering of FRET-based biosensors. PMID:22677394

78 FR 48078 - Proposed Amendment of Class E Airspace; Tazewell, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-07

...: Federal Aviation Administration (FAA), DOT. ACTION: Notice of proposed rulemaking (NPRM). SUMMARY: This action proposes to amend Class E Airspace at Tazewell, TN, as new Standard Instrument Approach Procedures have been developed at New Tazewell Municipal Airport. This action would enhance the safety and...

78 FR 43971 - Amendment of Class E Airspace; Tri-Cities, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-07-23

...-Cities Regional Airport, Tri-Cities, TN. Exclusionary language was omitted in the final rule published in... herein to include the corrective language. Since the regulatory text, as currently described, penetrates... FAA has determined that this regulation only involves an established body of technical regulations for...

Rapid and efficient introduction of a foreign gene into bacterial artificial chromosome-cloned varicella vaccine by Tn7-mediated site-specific transposition

DOE Office of Scientific and Technical Information (OSTI.GOV)

Somboonthum, Pranee; Koshizuka, Tetsuo; Okamoto, Shigefumi

2010-06-20

Using a rapid and reliable system based on Tn7-mediated site-specific transposition, we have successfully constructed a recombinant Oka varicella vaccine (vOka) expressing the mumps virus (MuV) fusion protein (F). The backbone of the vector was our previously reported vOka-BAC (bacterial artificial chromosome) genome. We inserted the transposon Tn7 attachment sequence, LacZ{alpha}-mini-attTn7, into the region between ORF12 and ORF13 to generate a vOka-BAC-Tn genome. The MuV-F expressing cassette was transposed into the vOka-BAC genome at the mini-attTn7 transposition site. MuV-F protein was expressed in recombinant virus, rvOka-F infected cells. In addition, the MuV-F protein was cleaved in the rvOka-F infected cellsmore » as in MuV-infected cells. The growth of rvOka-F was similar to that of the original recombinant vOka without the F gene. Thus, we show that Tn7-mediated transposition is an efficient method for introducing a foreign gene expression cassette into the vOka-BAC genome as a live virus vector.« less

Carcinoma autoantigens T and Tn and their cleavage products interact with Gal/GalNAc-specific receptors on rat Kupffer cells and hepatocytes.

PubMed

Schlepper-Schäfer, J; Springer, G F

1989-10-09

We studied interactions of isolated Thomsen-Friedenreich (T)- and Tn-specific glycoproteins with the Gal/GalNAc-specific receptors on rat Kupffer cells and compared them to those with rat hepatocytes. Immunoreactive T and Tn are specific pancarcinoma epitopes. Electron microscopy of gold-labelled T and Tn antigens revealed their specific binding to Kupffer cells, followed by their uptake via the coated pit/vesicle pathway of receptor-mediated endocytosis. Preincubation of Kupffer cells with GalNAc and GalNAc-BSA, but not GlcNAc or GlcNAc-BSA specifically inhibited binding of the T and Tn glycoproteins. Desialylated, isologous erythrocytes (T RBC) are known to bind to the Gal/GalNAc receptors of rat Kupffer cells and hepatocytes. This attachment was specifically inhibited by T and Tn in a concentration-dependent manner: 50% T RBC-Kupffer cell contacts were inhibited at 8.5.10(-6) mM T and 8.5.10(-5) mM Tn antigen concentrations, respectively. The corresponding figures for hepatocytes were 6.10(-6) mM T and 1.2.10(-6) mM Tn antigen. Amino-terminal cleavage products of the T glycoprotein, possessing clusters terminating in non-reducing Gal/GalNAc, inhibited T RBC binding to Kupffer cells and hepatocytes usually at 10(-2) to 10(-5) mM concentrations, whereas GalNAc, galactose and galactose glycosides inhibited at millimolar concentrations. Galactose-unrelated carbohydrates were inactive at concentrations greater than or equal to 50 mM.

75 FR 65584 - Proposed Amendment of Class E Airspace; Savannah, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-26

...-1047; Airspace Docket No. 10-ASO-37] Proposed Amendment of Class E Airspace; Savannah, TN AGENCY...; Telephone: 1- 800-647-5527; Fax: 202-493-2251. You must identify the Docket Number FAA-2010-1047; Airspace... the proposal. Communications should identify both docket numbers (FAA Docket No. FAA-2010-1047...

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration.

PubMed

Stewart, Barry J; Wardle, Simon J; Haniford, David B

2002-08-15

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes.

IHF-independent assembly of the Tn10 strand transfer transpososome: implications for inhibition of disintegration

PubMed Central

Stewart, Barry J.; Wardle, Simon J.; Haniford, David B.

2002-01-01

The frequency of DNA transposition in transposition systems that employ a strand transfer step may be significantly affected by the occurrence of a disintegration reaction, a reaction that reverses the strand transfer event. We have asked whether disintegration occurs in the Tn10 transposition system. We show that disintegration substrates (substrates constituting one half of the strand transfer product) are assembled into a transpososome that mimics the strand transfer intermediate. This strand transfer transpososome (STT) does appear to support an intermolecular disintegration reaction, but only at a very low level. Strikingly, assembly of the STT is not dependent on IHF, a host protein that is required for de novo assembly of all previously characterized Tn10 transpososomes. We suggest that disintegration substrates are able to form both transposon end and target type contacts with transposase because of their enhanced conformational flexibility. This probably allows the conformation of DNA within the complex that prevents the destructive disintegration reaction, and is responsible for relaxing the DNA sequence requirements for STT formation relative to other Tn10 transpososomes. PMID:12169640

KPC-4 Is Encoded within a Truncated Tn4401 in an IncL/M Plasmid, pNE1280, Isolated from Enterobacter cloacae and Serratia marcescens

PubMed Central

Bryant, Kendall A.; Van Schooneveld, Trevor C.; Thapa, Ishwor; Bastola, Dhundy; Williams, Laurina O.; Safranek, Thomas J.; Hinrichs, Steven H.; Rupp, Mark E.

2013-01-01

We describe the transfer of blaKPC-4 from Enterobacter cloacae to Serratia marcescens in a single patient. DNA sequencing revealed that KPC-4 was encoded on an IncL/M plasmid, pNE1280, closely related to pCTX-M360. Further analysis found that KPC-4 was encoded within a novel Tn4401 element (Tn4401f) containing a truncated tnpA and lacking tnpR, ISKpn7 left, and Tn4401 IRL-1, which are conserved in other Tn4401 transposons. This study highlights the continued evolution of Tn4401 transposons and movement to multiple plasmid backbones that results in acquisition by multiple species of Gram-negative bacilli. PMID:23070154

Comparison of the new high sensitive cardiac troponin T with myoglobin, h-FABP and cTnT for early identification of myocardial necrosis in the acute coronary syndrome.

PubMed

Kurz, Kerstin; Giannitsis, Evangelos; Becker, Maike; Hess, Georg; Zdunek, Dietmar; Katus, Hugo A

2011-03-01

We sought to determine the performance of the new high sensitivity cardiac troponin T assay (TnThs) for early diagnosis of myocardial infarction in patients with suspected acute coronary syndrome (ACS) and compare it with the fourth generation cTnT assay, myoglobin and heart-type fatty acid binding protein (h-FABP). Ninety-four patients with diagnosis of suspected ACS without ST-segment elevation admitted to our chest pain unit were included. Patients were divided according to time from onset of symptoms to presentation into an early presenter group (<4 h) and a late presenter group (≥4 h). A median of six samples (range 2-8) were available per patient. The diagnostic performance of TnThs was assessed using ROC analysis. Areas under the curve (AUC) of baseline and follow-up results of TnThs, cTnT, myoglobin, and h-FABP were compared using c statistics. The TnThs assay allows an excellent prediction of non-ST-segment elevation myocardial infarction (non-STEMI) at presentation, particularly among late presenters. A follow-up sample improves diagnostic performance in a time-dependent manner. The AUC of TnThs was superior to cTnT at all time points. The performance of TnThs was at least as good as myoglobin and h-FABP at presentation and during follow-up. A baseline sample of TnThs allows an earlier prediction of non-STEMI than the less sensitive and precise fourth generation cTnT assay. Probably, this excellent performance of TnThs at baseline and follow-up could obviate the need for other early markers of necrosis in future.

Rainfall, streamflow, and water-quality data for five small watersheds, Nashville, Tennessee, 1990-92

USGS Publications Warehouse

Outlaw, George S.; Hoos, Anne B.; Pankey, John T.

1994-01-01

Rainfall, streamflow, and water-quality data were collected furing storm conditions at five urban watersheds in Nashville, Tennessee. These data can be used to build a database for developing predictive models of the relations between storm- water quality and land use, storm characteristics, and seasonal variations. The primary land and mix of land uses was different for each watershed. Stormwater samples were collected during three storms at each watershed and analyzed for selected volatile, acidic and base/neutral organic compounds; organic pesticides; trace metals; conventional pollutants; and several physical properties. Storm loads were computed for all constituents and properties with event mean concentration above the minimum reporting level. None of the samples con- tained acidic organic compounds at concentrations above the minimum reporting levels. Several constituents in each of the other categories, however, were present at concentrations above the minimum reporting level. For 21 of these constituents, water-quality criteria have been pro- mulgated by the State of Tennessee. For only 8 of the 21 did the value exceed the most restrictive of the criteria: pyrene, dieldrin, and mercury concen- trations and counts of fecal coliform exceeded the criteria for recreational use, copper and zinc concentrations and pH value exceeded the criteria for fish and aquatic life, and lead concentrations exceeded the criteria for domestic supply.

Youth Violence: Developing Local and State Solutions. Hearings before the Subcommittee on Youth Violence of the Committee on the Judiciary. United States Senate, One Hundred Fourth Congress, Second Session on Focusing on Youth Violence and Developing Local and State Solutions (Memphis and Nashville, TN, February 15 and 16, 1996).

ERIC Educational Resources Information Center

Congress of the U.S., Washington, DC. Senate Committee on the Judiciary.

This hearing focused on youth violence and the importance of developing local and state solutions. Senator Fred Thompson made an introductory statement. This was followed by four panels on each of 2 days of testimony. Day one's first panel included involved students and business people from Memphis, TN. The second panel included two Tennessee…

Radioprotection of the Brain White Matter by Mn(III) N-Butoxyethylpyridylporphyrin-Based Superoxide Dismutase Mimic MnTnBuOE-2-PyP5+

PubMed Central

Weitzel, Douglas H.; Tovmasyan, Artak; Ashcraft, Kathleen A.; Rajic, Zrinka; Weitner, Tin; Liu, Chunlei; Li, Wei; Buckley, Anne F.; Prasad, Mark R.; Young, Kenneth H.; Rodriguiz, Ramona M.; Wetsel, William C.; Peters, Katherine B.; Spasojevic, Ivan; Herndon, James E.; Batinic-Haberle, Ines; Dewhirst, Mark W.

2015-01-01

Cranial irradiation is a standard therapy for primary and metastatic brain tumors. A major drawback of radiotherapy (RT), however, is long-term cognitive loss that affects quality of life. Radiation-induced oxidative stress in normal brain tissue is thought to contribute to cognitive decline. We evaluated the effectiveness of a novel mimic of superoxide dismutase enzyme (SOD), MnTnBuOE-2-PyP5+ (Mn(III) meso-tetrakis(N-n-butoxyethylpyridinium-2-yl)porphyrin), to provide long-term neuroprotection following 8 Gy of whole brain irradiation. Long-term RT damage can only be assessed by brain imaging and neurocognitive studies. C57BL/6J mice were treated with MnTnBuOE-2-PyP5+ before and after RT and evaluated three months later. At this time point, drug concentration in the brain was 25 nmol/L. Mice treated with MnTnBuOE-2-PyP5+/RT exhibited MRI evidence for myelin preservation in the corpus callosum compared with saline/RT treatment. Corpus callosum histology demonstrated a significant loss of axons in the saline/RT group that was rescued in the MnTnBuOE-2-PyP5+/RT group. In addition, the saline/RT groups exhibited deficits in motor proficiency as assessed by the rotorod test and running wheel tests. These deficits were ameliorated in groups treated with MnTnBuOE-2-PyP5+/RT. Our data demonstrate that MnTnBuOE-2-PyP5+ is neuroprotective for oxidative stress damage caused by radiation exposure. In addition, glioblastoma cells were not protected by MnTnBuOE-2-PyP5+ combination with radiation in vitro. Likewise, the combination of MnTnBuOE-2-PyP5+ with radiation inhibited tumor growth more than RT alone in flank tumors. In summary, MnTnBuOE-2-PyP5+ has dual activity as a neuroprotector and a tumor radiosensitizer. Thus, it is an attractive candidate for adjuvant therapy with RT in future studies with patients with brain cancer. PMID:25319393

The relationship between the expression of TN and the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis

PubMed Central

Jiang, Guohua; Zhang, Yinshun; Sun, Xianjie

2018-01-01

Abstract Background: This study was conducted with the aim to investigate the relationship between Tetranectin (TN) and efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis. Methods: Ninety-two patients with traumatic kyphosis admitted in our hospital from February 2014 to June 2016 were included whose serum TN levels were examined by ELISA. Using the mean level of TN as cut-off value, patients were classified into TN high level group (group I) and TN low level group (group II). The observation indexes, including operation time, intra-operational loss of blood, Cobb angle, postoperative complications and recurrence rate of kyphosis within post-operational 6 months were recorded for comparison. Results: TN level was significantly higher in group I [(6.19 ± 0.33) μmol/L] than that in group II [(5.29 ± 0.34) μmol/L] (P < .05). There was no significant difference in average age, sex, lesion site and average time from injury to operation between the two groups (all P > 0.05). Compared to group II, operation time in group I was significantly shortened (5.02 ± 1.15 VS 4.58 ± 0.53, P = .023), the intra-operational loss of blood decreased (2418.56 ± 362.06 VS 2235.84 ± 325.63, P = .013), post-operational Cobb angle decreased (11.10 ± 1.31 VS 6.93 ± 1.04, P = .000), and the incidence of postoperative complications (nail-breaking, rod-breaking and looseness) and recurrence rate decreased (18.8% VS 4.5%, P = .036; 10.4% VS 0.0%, P = .028). Conclusion: Serum TN level is proved to be related to the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis, and may serve as a possible indicator for clinical treatment. PMID:29384840

The relationship between the expression of TN and the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis.

PubMed

Jiang, Guohua; Zhang, Yinshun; Sun, Xianjie

2018-02-01

This study was conducted with the aim to investigate the relationship between Tetranectin (TN) and efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis. Ninety-two patients with traumatic kyphosis admitted in our hospital from February 2014 to June 2016 were included whose serum TN levels were examined by ELISA. Using the mean level of TN as cut-off value, patients were classified into TN high level group (group I) and TN low level group (group II). The observation indexes, including operation time, intra-operational loss of blood, Cobb angle, postoperative complications and recurrence rate of kyphosis within post-operational 6 months were recorded for comparison. TN level was significantly higher in group I [(6.19 ± 0.33) μmol/L] than that in group II [(5.29 ± 0.34) μmol/L] (P < .05). There was no significant difference in average age, sex, lesion site and average time from injury to operation between the two groups (all P > 0.05). Compared to group II, operation time in group I was significantly shortened (5.02 ± 1.15 VS 4.58 ± 0.53, P = .023), the intra-operational loss of blood decreased (2418.56 ± 362.06 VS 2235.84 ± 325.63, P = .013), post-operational Cobb angle decreased (11.10 ± 1.31 VS 6.93 ± 1.04, P = .000), and the incidence of postoperative complications (nail-breaking, rod-breaking and looseness) and recurrence rate decreased (18.8% VS 4.5%, P = .036; 10.4% VS 0.0%, P = .028). Serum TN level is proved to be related to the efficiency of posterior spinal V osteotomy in patients with traumatic kyphosis, and may serve as a possible indicator for clinical treatment.

Tn (N-acetyl-d-galactosamine-O-serine/threonine) immunization protects against hyperoxia-induced lung injury in adult mice through inhibition of the nuclear factor kappa B activity.

PubMed

Chen, Chung-Ming; Hwang, Jaulang; Chou, Hsiu-Chu; Shiah, Her-Shyong

2018-06-01

Prolonged hyperoxia exposure leads to inflammation and acute lung injury. Since hyperoxia activates nuclear factor kappa B (NF-κB) and proinflammatory mediators in lung fibroblasts and murine lungs, and proinflammatory cytokines upregulate Tn (N-acetyl-d-galactosamine-O-serine/threonine) expression in human gingival fibroblasts. We hypothesized connections exist between Tn expression and inflammation regulation. Thus, we immunized adult mice with Tn antigen to examine whether Tn vaccine can protect against hyperoxia-induced lung injury by inhibiting NF-κB activity and cytokine expression through the action of anti-Tn antibodies. Five-week-old female C57BL/6NCrlBltw mice were subcutaneously immunized with Tn antigen four times at biweekly intervals, and one additional immunization was performed at 1 week after the fourth immunization. Four days after the last immunization, mice were exposed to room air (RA) or hyperoxia (100% O 2 ) for up to 96 h. Four study groups were examined: carrier protein + RA (n = 6), Tn vaccine + RA (n = 6), carrier protein + O 2 (n = 6), and Tn vaccine + O 2 (n = 5). We observed that hyperoxia exposure reduced body weight, increased alveolar protein and cytokine (interleukin-6 and tumor necrosis factor-α) levels, increased mean linear intercept (MLI) values and lung injury scores, and increased lung NF-κB activity. By contrast, Tn immunization increased serum anti-Tn antibody titers and reduced the cytokine levels, MLI values, and lung injury scores. Furthermore, the alleviation of lung injury was accompanied by a reduction in NF-κB activity. Therefore, we proposed that Tn immunization attenuates hyperoxia-induced lung injury in adult mice by inhibiting the NF-κB activity. Copyright © 2018 Elsevier B.V. All rights reserved.

In-house preparation of lectin panel and detection of Tn polyagglutination.

PubMed

Das, Sudipta Sekhar

2015-01-01

Polyagglutination is a condition in which red cells are agglutinated by ABO-compatible adult human sera, but not by cord blood sera and may be acquired or inherited. Lectins are invaluable reagents in the investigation of red cells polyagglutination. We prepared in-house lectin panel and confirmed Tn polyagglutination in a pregnant lady. The lady was anemic and refused blood transfusion elsewhere due to serological discrepancy. We found ABO discrepancy and an incompatible minor cross-match in the initial investigation and suspected polyagglutination. Confirmation of polyagglutination was done using adult and cord sera. We then used the in-house lectin panels to detect the type of polyagglutination. The agglutination pattern with the various lectins was suggestive of Tn polyagglutination, which was further supported by the enzyme study. Most blood banks in India lack commercial lectin panels because of cost and procurement difficulty. Lectins play an important role in the diagnosis and differentiation of polyagglutination and immunohematological management of patient. The important and basic lectins can be prepared in-house using specific raw seeds following standardized protocol.

Novel Structure of Enterococcus faecium-Originated ermB-Positive Tn1546-Like Element in Staphylococcus aureus.

PubMed

Wan, Tsai-Wen; Hung, Wei-Chun; Tsai, Jui-Chang; Lin, Yu-Tzu; Lee, Hao; Hsueh, Po-Ren; Lee, Tai-Fen; Teng, Lee-Jene

2016-10-01

We determined the resistance determinants in 274 erythromycin-resistant methicillin-susceptible Staphylococcus aureus (MSSA) isolates during a 13-year period, 2000 to 2012. The resistance phenotypes, inducible macrolide-lincosamide-streptogramin (iMLS), constitutive MLS (cMLS), and macrolide-streptogramin (MS) resistance phenotypes, were examined by a double-disk diffusion D test. The ermB gene was more frequent (35%; 97/274) than ermC (27%; 75/274) or ermA (21%; 58/274). All 97 ermB-positive isolates harbored Tn551 and IS1216V The majority (89/97) of ermB-positive isolates displayed the cMLS phenotype and carried mobile element structure (MES)-like structures, which has been previously reported in sequence type 59 (ST59) methicillin-resistant S. aureus (MRSA). The remaining 8 ermB-carrying isolates, belonging to ST7 (n = 4), ST5 (n = 3), and ST59 (n = 1), were sasK intact and did not carry MES-like structures. Unlike a MES-like structure that was located on the chromosome, the ermB elements on sasK-intact isolates were located on plasmids by S1 nuclease pulsed-field gel electrophoresis (PFGE) analysis and conjugation tests. Sequence data for the ermB-containing region (14,566 bp) from ST59 NTUH_3874 revealed that the best match was a Tn1546-like element in plasmid pMCCL2 DNA (GenBank accession number AP009486) of Macrococcus caseolyticus Tn1546 is recognized as an enterococcal transposon and was known from the vancomycin resistance gene cluster in vancomycin-resistant Enterococcus (VRE). So far, acquisitions of Tn1546 in S. aureus have occurred in clonal complex 5 (CC5) MRSA, but not in MSSA. This is the first report that MSSA harbors an Enterococcus faecium-originated ermB-positive Tn1546-like element located on a plasmid. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes

PubMed Central

Bevacqua, R. J.; Fernandez-Martin, R.; Canel, N. G.; Gibbons, A.; Texeira, D.; Lange, F.; Vans Landschoot, G.; Savy, V.; Briski, O.; Hiriart, M. I.; Grueso, E.; Ivics, Z.; Taboga, O.; Kues, W. A.; Ferraris, S.

2017-01-01

Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice and pigs. In this work, Tn5 and Sleeping Beauty (SB) transposon systems were evaluated for transgenesis by simple cytoplasmic injection in livestock zygotes. In the case of Tn5, the transposome complex of transposon nucleic acid and Tn5 protein was injected. In the case of SB, the supercoiled plasmids encoding a transposon and the SB transposase were co-injected. In vitro produced bovine zygotes were used to establish the cytoplasmic injection conditions. The in vitro cultured blastocysts were evaluated for reporter gene expression and genotyped. Subsequently, both transposon systems were injected in seasonally available ovine zygotes, employing transposons carrying the recombinant human factor IX driven by the beta-lactoglobulin promoter. The Tn5 approach did not result in transgenic lambs. In contrast, the Sleeping Beauty injection resulted in 2 lambs (29%) carrying the transgene. Both animals exhibited cellular mosaicism of the transgene. The extraembryonic tissues (placenta or umbilical cord) of three additional animals were also transgenic. These results show that transpositional transgenesis by cytoplasmic injection of SB transposon components can be applied for the production of transgenic lambs of pharmaceutical interest. PMID:28301581

Temperature-dependent instability of the cTnI subunit in NIST SRM2921 characterized by tryptic peptide mapping.

PubMed

van der Burgt, Yuri E M; Cobbaert, Christa M; Dalebout, Hans; Smit, Nico; Deelder, André M

2012-08-01

In this study temperature-dependent instability of the cTnI subunit of the three-protein complex NIST SRM2921 was demonstrated using a mass spectrometric tryptic peptide mapping approach. The results were compared to the cTnI subunit obtained as a protein standard from Calbiochem with identical amino acid sequence. Both the three-protein complex from NIST as well as the cTnI subunit were incubated at elevated temperatures and then evaluated with respect to the primary sequence. The corresponding peptide maps were analyzed using LC-MS/MS. From a Mascot database search in combination with "semiTrypsin" tolerance it was found that two peptide backbone cleavages had occurred in subunit cTnI in NIST SRM2921 material upon incubation at 37°C, namely between amino acids at 148/149 and 194/195. The Calbiochem standard did not show increased levels of "unexpected" peptides in tryptic peptide maps. One of the two peptide backbone cleavages could also be monitored using a "single-step" MALDI-MS approach, i.e. without the need for peptide separation. The amount of degradation appeared rather constant in replicate temperature-instability experiments. However, for accurate quantification internal labelled standards are needed. Copyright © 2012 Elsevier B.V. All rights reserved.

Adjustment of regional regression models of urban-runoff quality using data for Chattanooga, Knoxville, and Nashville, Tennessee

USGS Publications Warehouse

Hoos, Anne B.; Patel, Anant R.

1996-01-01

Model-adjustment procedures were applied to the combined data bases of storm-runoff quality for Chattanooga, Knoxville, and Nashville, Tennessee, to improve predictive accuracy for storm-runoff quality for urban watersheds in these three cities and throughout Middle and East Tennessee. Data for 45 storms at 15 different sites (five sites in each city) constitute the data base. Comparison of observed values of storm-runoff load and event-mean concentration to the predicted values from the regional regression models for 10 constituents shows prediction errors, as large as 806,000 percent. Model-adjustment procedures, which combine the regional model predictions with local data, are applied to improve predictive accuracy. Standard error of estimate after model adjustment ranges from 67 to 322 percent. Calibration results may be biased due to sampling error in the Tennessee data base. The relatively large values of standard error of estimate for some of the constituent models, although representing significant reduction (at least 50 percent) in prediction error compared to estimation with unadjusted regional models, may be unacceptable for some applications. The user may wish to collect additional local data for these constituents and repeat the analysis, or calibrate an independent local regression model.

MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells

PubMed Central

Kosmacek, Elizabeth A.; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley, Rebecca E.

2016-01-01

Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers. PMID:27119354

MnTnBuOE-2-PyP protects normal colorectal fibroblasts from radiation damage and simultaneously enhances radio/chemotherapeutic killing of colorectal cancer cells.

PubMed

Kosmacek, Elizabeth A; Chatterjee, Arpita; Tong, Qiang; Lin, Chi; Oberley-Deegan, Rebecca E

2016-06-07

Manganese porphyrins have been shown to be potent radioprotectors in a variety of cancer models. However, the mechanism as to how these porphyrins protect normal tissues from radiation damage still remains largely unknown. In the current study, we determine the effects of the manganese porphyrin, MnTnBuOE-2-PyP, on primary colorectal fibroblasts exposed to irradiation. We found that 2 Gy of radiation enhances the fibroblasts' ability to contract a collagen matrix, increases cell size and promotes cellular senesence. Treating fibroblasts with MnTnBuOE-2-PyP significantly inhibited radiation-induced collagen contraction, preserved cell morphology and also inhibited cellular senescence. We further showed that MnTnBuOE-2-PyP enhanced the overall viability of the fibroblasts following exposure to radiation but did not protect colorectal cancer cell viability. Specifically, MnTnBuOE-2-PyP in combination with irradiation, caused a significant decrease in tumor clonogenicity. Since locally advanced rectal cancers are treated with chemoradiation therapy followed by surgery and non-metastatic anal cancers are treated with chemoradiation therapy, we also investigated the effects of MnTnBuOE-2-PyP in combination with radiation, 5-fluorouracil with and without Mitomycin C. We found that MnTnBuOE-2-PyP in combination with Mitomycin C or 5-fluorouracil further enhances those compounds' ability to suppress tumor cell growth. When MnTnBuOE-2-PyP was combined with the two chemotherapeutics and radiation, we observed the greatest reduction in tumor cell growth. Therefore, these studies indicate that MnTnBuOE-2-PyP could be used as a potent radioprotector for normal tissue, while at the same time enhancing radiation and chemotherapy treatment for rectal and anal cancers.

78 FR 58489 - Proposed Amendment of Class E Airspace; McMinnville, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-24

... County Memorial Airport, TN (Lat. 35[deg]41'55'' N., long. 85[deg]50'38'' W.) Columbia River Park... Flight Rules (IFR) operations at the airport. DATES: Comments must be received on or before November 8, 2013. ADDRESSES: Send comments on this rule to: U. S. Department of Transportation, Docket Operations...

Novel Tn916-like elements confer aminoglycoside/macrolide co-resistance in clinical isolates of Streptococcus gallolyticus ssp. gallolyticus.

PubMed

Kambarev, Stanimir; Pecorari, Frédéric; Corvec, Stéphane

2018-02-09

Streptococcus gallolyticus ssp. gallolyticus (Sgg) is a commensal bacterium and an opportunistic pathogen. In humans it has been clinically associated with the incidence of colorectal cancer (CRC) and epidemiologically recognized as an emerging cause of infective endocarditis (IE). The standard therapy of Sgg includes the administration of a penicillin in combination with an aminoglycoside. Even though penicillin-resistant isolates have still not been reported, epidemiological studies have shown that this microbe is a reservoir of multiple acquired genes, conferring resistance to tetracyclines, aminoglycosides, macrolides and glycopeptides. However, the underlying antibiotic resistance mobilome of Sgg remains poorly understood. To investigate the mobile genetic basis of antibiotic resistance in multiresistant clinical Sgg. Isolate NTS31106099 was recovered from a patient with IE and CRC at Nantes University Hospital, France and studied by Illumina WGS and comparative genomics. Molecular epidemiology of the identified mobile element(s) was performed using antibiotic susceptibility testing (AST), PCR, PFGE and WGS. Mobility was investigated by PCR and filter mating. Two novel conjugative transposons, Tn6263 and Tn6331, confer aminoglycoside/macrolide co-resistance in clinical Sgg. They display classical family Tn916/Tn1545 modular architecture and harbour an aph(3')-III→sat4→ant(6)-Ia→erm(B) multiresistance gene cluster, related to pRE25 of Enterococcus faecium. These and/or closely related elements are highly prevalent among genetically heterogeneous clinical isolates of Sgg. Previously unknown Tn916-like mobile genetic elements conferring aminoglycoside/macrolide co-resistance make Sgg, collectively with other gut Firmicutes such as enterococci and eubacteria, a potential laterally active reservoir of these antibiotic resistance determinants among the mammalian gastrointestinal microbiota. © The Author(s) 2018. Published by Oxford University Press on behalf

Interruption of capsule production in Streptococcus pneumonia serotype 3 by insertion of transposon Tn916.

PubMed Central

Watson, D A; Musher, D M

1990-01-01

Transposon Tn916 mutagenesis was used to produce mutant strains of Streptococcus pneumoniae serotype 3 that lacked only a polysaccharide capsule. Southern blotting, DNA-DNA hybridization, and immunochemical analyses demonstrated that the presence of a single copy of Tn916 was sufficient to produce unencapsulation. The 50% lethal dose for such mutants was greater than 5 x 10(7) CFU, as opposed to a 50% lethal dose of 1 CFU for wild-type strains. These experiments outline an effective method for targeting genes in S. pneumoniae by transposon interruption and provide molecular evidence to support the longstanding hypothesis that the capsule is the principal virulence factor in this pathogen. Images PMID:2167295

Characterization of Tn1546 in vancomycin-resistant Enterococcus faecium isolated from canine urinary tract infections: evidence of gene exchange between human and animal enterococci.

PubMed

Simjee, S; White, D G; McDermott, P F; Wagner, D D; Zervos, M J; Donabedian, S M; English, L L; Hayes, J R; Walker, R D

2002-12-01

Thirty-five enterococcal isolates were recovered from dogs diagnosed with urinary tract infections at the Michigan State University Veterinary Teaching Hospital over a 2-year period (1996 to 1998). Isolated species included Enterococcus faecium (n = 13), Enterococcus faecalis (n = 7), Enterococcus gallinarum (n = 11), and Enterococcus casseliflavus (n = 4). Antimicrobial susceptibility testing revealed several different resistance phenotypes, with the majority of the enterococcal isolates exhibiting resistance to three or more antibiotics. One E. faecium isolate, CVM1869, displayed high-level resistance to vancomycin (MIC > 32 micro g/ml) and gentamicin (MIC > 2,048 micro g/ml). Molecular analysis of this isolate revealed the presence of Tn1546 (vanA), responsible for high-level vancomycin resistance, and Tn5281 carrying aac6'-aph2", conferring high-level aminoglycoside resistance. Pulsed-field gel electrophoresis analysis revealed that CVM1869 was a canine E. faecium clone that had acquired Tn1546, perhaps from a human vancomycin-resistant E. faecium. Transposons Tn5281 and Tn1546 were located on two different conjugative plasmids. Sequence analysis revealed that in Tn1546, ORF1 had an 889-bp deletion and an IS1216V insertion at the 5' end and an IS1251 insertion between vanS and vanH. To date, this particular form of Tn1546 has only been described in human clinical vancomycin-resistant enterococcus isolates unique to the United States. Additionally, this is the first report of a vancomycin-resistant E. faecium isolated from a companion animal in the United States.

77 FR 59994 - Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal

Federal Register 2010, 2011, 2012, 2013, 2014

2012-10-01

... NUCLEAR REGULATORY COMMISSION [Docket No. 70-143; NRC-2009-0435] Nuclear Fuel Services, Inc., Erwin, TN; Issuance of License Renewal AGENCY: U.S. Nuclear Regulatory Commission. ACTION: Notice of... Nuclear Material Safety and Safeguards, U.S. Nuclear Regulatory Commission, Washington, DC 20555-0001...

Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation.

PubMed

Vedantam, Gayatri; Knopf, Sarah; Hecht, David W

2006-01-01

Tn5520 is the smallest known bacterial mobilizable transposon and was isolated from an antibiotic resistant Bacteroides fragilis clinical isolate. When a conjugation apparatus is provided in trans, Tn5520 is mobilized (transferred) efficiently within, and from, both Bacteroides spp. and Escherichia coli. Only two genes are present on Tn5520; one encodes an integrase, and the other a multifunctional mobilization (Mob) protein BmpH. BmpH is essential for Tn5520 mobility. The focus of this study was to identify the Tn5520 origin of conjugative transfer (oriT) and to study BmpH-oriT binding. We delimited the functional Tn5520 oriT to a 71 bp sequence upstream of the bmpH gene. A plasmid vector harbouring this minimal 71 bp oriT was mobilized at the same frequency as that of intact Tn5520. The minimal oriT contains one 17 bp inverted repeat (IR) sequence. We constructed and tested multiple IR mutants and showed that the IR was essential in its entirety for mobilization. A nick site sequence (5'-GCTAC-3') was also identified within the minimal oriT; this sequence resembled nick sites found in plasmids of Gram positive origin. We further showed that mutation of a highly conserved GC dinucleotide in the nick site sequence completely abolished mobilization. We also purified BmpH and showed that it specifically bound a Tn5520 oriT fragment in electrophoretic mobility shift assays. We also identified non-nick site sequences within the minimal oriT that were essential for mobilization. We hypothesize that transposon-based single Mob protein systems may contribute to efficient gene dissemination from Bacteroides spp., because fewer DNA processing proteins are required for relaxosome formation.

Morphological and functional diversity of ectomycorrhizal fungi on Roan Mountain (NC/TN)

Treesearch

Claire Bird; Coleman McCleneghan

2005-01-01

A comparison of ectomycorrhizal morphotypes and hypogeous fungi (truffles and false-truffles) in northern hardwood and spruce-fir forests on Roan Mountain (NC/TN) was performed to increase our knowledge of the fungal communities in the Southern Appalachian high elevation forests. These forests are home to an endangered subspecies and mycophagist, the Carolina northern...

Recruitment of CRISPR-Cas systems by Tn7-like transposons.

PubMed

Peters, Joseph E; Makarova, Kira S; Shmakov, Sergey; Koonin, Eugene V

2017-08-29

A survey of bacterial and archaeal genomes shows that many Tn7-like transposons contain minimal type I-F CRISPR-Cas systems that consist of fused cas8f and cas5f , cas7f , and cas6f genes and a short CRISPR array. Several small groups of Tn7-like transposons encompass similarly truncated type I-B CRISPR-Cas. This minimal gene complement of the transposon-associated CRISPR-Cas systems implies that they are competent for pre-CRISPR RNA (precrRNA) processing yielding mature crRNAs and target binding but not target cleavage that is required for interference. Phylogenetic analysis demonstrates that evolution of the CRISPR-Cas-containing transposons included a single, ancestral capture of a type I-F locus and two independent instances of type I-B loci capture. We show that the transposon-associated CRISPR arrays contain spacers homologous to plasmid and temperate phage sequences and, in some cases, chromosomal sequences adjacent to the transposon. We hypothesize that the transposon-encoded CRISPR-Cas systems generate displacement (R-loops) in the cognate DNA sites, targeting the transposon to these sites and thus facilitating their spread via plasmids and phages. These findings suggest the existence of RNA-guided transposition and fit the guns-for-hire concept whereby mobile genetic elements capture host defense systems and repurpose them for different stages in the life cycle of the element.

An efficient (t,n) threshold quantum secret sharing without entanglement

NASA Astrophysics Data System (ADS)

Qin, Huawang; Dai, Yuewei

2016-04-01

An efficient (t,n) threshold quantum secret sharing (QSS) scheme is proposed. In our scheme, the Hash function is used to check the eavesdropping, and no particles need to be published. So the utilization efficiency of the particles is real 100%. No entanglement is used in our scheme. The dealer uses the single particles to encode the secret information, and the participants get the secret through measuring the single particles. Compared to the existing schemes, our scheme is simpler and more efficient.

Free-electron laser wavelength-selective materials alteration and photoexcitation spectroscopy

NASA Astrophysics Data System (ADS)

Tolk, N. H.; Albridge, R. G.; Barnes, A. V.; Barnes, B. M.; Davidson, J. L.; Gordon, V. D.; Margaritondo, G.; McKinley, J. T.; Mensing, G. A.; Sturmann, J.

1996-10-01

The free-electron laser (FEL) has become an important tool for producing high-intensity photon beams, especially in the infrared. Synchrotron radiation's primary spectral domains are in the ultraviolet and X-ray region. FEL's are therefore excellent complimentary facilities to synchrotron radiation sources. While FEL's have seen only limited use in experimentation, recently developed programs at Vanderbilt University in Nashville, TN, are swiftly rectifying this situation. This review paper examines practical experience obtained through pioneering programs using the Vanderbilt FEL, which currently hosts one of the largest FEL materials research programs. Results will be discussed in three areas: two-photon absorption in germanium, FEL-assisted internal photoemission measurements of interface energy barriers (FELIPE), and wavelength-specific laser diamond ablation.

UTSI/CFFF MHD PROGRAM COMPLETION AND RELATED ACTIVITY

DOE Office of Scientific and Technical Information (OSTI.GOV)

Joel W. Muehlhauser

2003-08-01

The Discharge Monitoring Reports (DMRs) for April, May and June, 2003, were prepared and submitted to the Tennessee Department of Environment and Conservation (TDEC). TVG Environmental, Inc. of Nashville, TN conducted the second semi-annual sampling event for the groundwater wells on the UTSI campus in mid-May, 2003. A report detailing the sampling and subsequent water analysis was forwarded to The Tennessee Department of Environment and Conservation in early June. Copies of that report were provided to DOE by Dr. Joel Muehlhauser. Contamination levels above state limits were found in all five wells sampled for at least one compound. TDEC willmore » be contacted next quarter to determine what subsequent actions are necessary.« less

Different Variations of Néel Temperature TN and Kondo Temperature TK in the Alloy System Ce(Ru1-xOsx)2Al10 under Uniaxial Pressure

NASA Astrophysics Data System (ADS)

Takeuchi, Takashi; Hayashi, Kyosuke; Umeo, Kazunori; Takabatake, Toshiro

2018-05-01

We report magnetic, transport, and specific-heat measurements for single crystals of the antiferromagnetic (AFM) Kondo semiconductor alloy series Ce(Ru1-xOsx)2Al10 (0 ≤ x ≤ 1), which crystallize into an orthorhombic structure. The specific-heat and resistivity data show that the isoelectronic substitution does not damage the hybridization gap or the AFM transition. The Kondo temperature TK increases linearly with x, whereas the Néel temperature TN exhibits a maximum value of 29.2 K for x = 0.71. Under increasing uniaxial pressure P || a, TN increases for x = 0 but decreases for x = 1, while TK increases in the entire range of x. Under P || b, in contrast, TN increases steadily in the whole range of x while TK remains unchanged for each x. The strongly anisotropic change in TN indicates the presence of another mechanism to enhance TN in this system in addition to the anisotropic hybridization of the 4f state with conduction bands.

78 FR 54569 - Special Local Regulation, Cumberland River, Mile 190.0 to 192.0; Nashville, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-05

... Festival. Entry into this area is prohibited unless specifically authorized by the Captain of the Port... participants and event personnel from the possible marine hazards present during the Festival. Delaying the... Festival. B. Basis and Purpose The Cumberland River Dragon Boat Festival takes place on the Cumberland...

Enhanced nitrogen removal from piggery wastewater with high NH4+ and low COD/TN ratio in a novel upflow microaerobic biofilm reactor.

PubMed

Meng, Jia; Li, Jiuling; Li, Jianzheng; Antwi, Philip; Deng, Kaiwen; Nan, Jun; Xu, Pianpian

2018-02-01

To enhance nutrient removal more cost-efficiently in microaerobic process treating piggery wastewater characterized by high ammonium (NH 4 + -N) and low chemical oxygen demand (COD) to total nitrogen (TN) ratio, a novel upflow microaerobic biofilm reactor (UMBR) was constructed and the efficiency in nutrient removal was evaluated with various influent COD/TN ratios and reflux ratios. The results showed that the biofilm on the carriers had increased the biomass in the UMBR and enhanced the enrichment of slow-growth-rate bacteria such as nitrifiers, denitrifiers and anammox bacteria. The packed bed allowed the microaerobic biofilm process perform well at a low reflux ratio of 35 with a NH 4 + -N and TN removal as high as 93.1% and 89.9%, respectively. Compared with the previously developed upflow microaerobic sludge reactor, the UMBR had not changed the dominant anammox approach to nitrogen removal, but was more cost-efficiently in treating organic wastewater with high NH 4 + -N and low COD/TN ratio. Copyright © 2017 Elsevier Ltd. All rights reserved.

Diagnostic value of analysis of H-FABP, NT-proBNP, and cTnI in heart function in children with congenital heart disease and pneumonia.

PubMed

Zhou, F-J; Zhou, C-Y; Tian, Y-J; Xiao, A-J; Li, P-L; Wang, Y-H; Jia, J-W

2014-01-01

To analyze the expression of heart-fatty acid-binding protein (H-FABP), N-terminal pro-brain natriuretic peptide (NT-proBNP), and cTnI in children with congenital heart disease (CHD) and pneumonia, and evaluate its diagnostic value in heart failure (HF). The levels of serum H-FABP, NT-proBNP, and cardiac troponin 1 (cTnI) were measured by immunoassays in 22 children with CHD, pneumonia, and HF (group I), 25 children with CHD and pneumonia (group II), and 25 healthy children without CHD or pneumonia (control group). The concentration and positive rate of serum H-FABP, NT-proBNP, and cTnI were significantly higher in group I than those in group II. Compared to control group, these indexes were increased in both group I and group II. There were statistical significant differences in the positive rate of NT-proBNP and cTnI but not H-FABP between groups of patients with different classes of heart function. The levels of H-FABP, NT-proBNP, and cTnI were correlated with heart function, and can be used for the diagnosis of early-stage HF in children with CHD.

[Improvement of butanol production by Escherichia coli via Tn5 transposon mediated mutagenesis].

PubMed

Lin, Zhao; Dong, Hongjun; Li, Yin

2015-12-01

For engineering an efficient butanol-producing Escherichia coli strain, many efforts have been paid on the known genes or pathways based on current knowledge. However, many genes in the genome could also contribute to butanol production in an unexpected way. In this work, we used Tn5 transposon to construct a mutant library including 1 196 strains in a previously engineered butanol-producing E. coli strain. To screen the strains with improved titer of butanol production, we developed a high-throughput method for pyruvate detection based on dinitrophenylhydrazine reaction using 96-well microplate reader, because pyruvate is the precursor of butanol and its concentration is inversely correlated with butanol in the fermentation broth. Using this method, we successfully screened three mutants with increased butanol titer. The insertion sites of Tn5 transposon was in the ORFs of pykA, tdk, and cadC by inverse PCR and sequencing. These found genes would be efficient targets for further strain improvement. And the genome scanning strategy described here will be helpful for other microbial cell factory construction.

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China

PubMed Central

Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Background and Objective Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Methods Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels(≥14ng/L) and patients with normal hs-cTnT levels (<14ng/L). Results The final analysis involved 84 patients (31 males; mean age, 61.6±12.2years), of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028), and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Conclusion Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies. PMID:26849554

Association of Elevated High Sensitivity Cardiac Troponin T(hs-cTnT) Levels with Hemorrhagic Transformation and 3-Month Mortality in Acute Ischemic Stroke Patients with Rheumatic Heart Disease in China.

PubMed

Liu, Junfeng; Wang, Deren; Xiong, Yao; Liu, Bian; Hao, Zilong; Tao, Wendan; Liu, Ming

2016-01-01

Elevated levels of high sensitivity cardiac troponin T (hs-cTnT) occur in a substantial proportion of patients with acute ischemic stroke (AIS) and can predict poor outcome and mortality after stroke. Whether elevated hs-cTnT levels can also predict hemorrhagic transformation (HT) or prognosis in AIS patients with rheumatic heart disease (RHD) remains unclear. Data from the Chengdu Stroke Registry on consecutive AIS patients with RHD admitted to West China Hospital within 1 month of stroke onset from October 2011 to February 2014 were examined. Clinico-demographic characteristics, HT, functional outcomes and stroke recurrence were compared between patients with elevated hs-cTnT levels (≥14 ng/L) and patients with normal hs-cTnT levels (<14 ng/L). The final analysis involved 84 patients (31 males; mean age, 61.6±12.2 years), of whom serum hs-cTnT levels were elevated in 58.3%. Renal impairment was independently associated with elevated hs-cTnT levels (OR 4.184, 95%CI 1.17 to 15.01, P = 0.028), and patients with elevated hs-cTnT levels were at significantly higher risk of HT, 3-month mortality and 3-month disability/mortality (all P≤0.029). After controlling for age, sex, hypertension, renal impairment and National Institutes of Health Stroke Scale score on admission, the risk of HT and 3-month mortality was, respectively, 4.0- and 5.5-fold higher in patients with elevated hs-cTnT levels than in patients with normal hs-cTnT levels. Elevated hs-cTnT levels are independently associated with HT and 3-month mortality in AIS patients with RHD. These results with a small cohort should be verified and extended in large studies.

An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus.

PubMed

Tuffin, I Marla; de Groot, Peter; Deane, Shelly M; Rawlings, Douglas E

2005-09-01

A transposon, TnAtcArs, that carries a set of arsenic-resistance genes was isolated from a strain of the moderately thermophilic, sulfur-oxidizing, biomining bacterium Acidithiobacillus caldus. This strain originated from a commercial plant used for the bio-oxidation of gold-bearing arsenopyrite concentrates. Continuous selection for arsenic resistance over many years had made the bacterium resistant to high concentrations of arsenic. Sequence analysis indicated that TnAtcArs is 12 444 bp in length and has 40 bp terminal inverted repeat sequences and divergently transcribed resolvase and transposase genes that are related to the Tn21-transposon subfamily. A series of genes consisting of arsR, two tandem copies of arsA and arsD, two ORFs (7 and 8) and arsB is situated between the resolvase and transposase genes. Although some commercial strains of At. caldus contained the arsDA duplication, when transformed into Escherichia coli, the arsDA duplication was unstable and was frequently lost during cultivation or if a plasmid containing TnAtcArs was conjugated into a recipient strain. TnAtcArs conferred resistance to arsenite and arsenate upon E. coli cells. Deletion of one copy of arsDA had no noticeable effect on resistance to arsenite or arsenate in E. coli. ORFs 7 and 8 had clear sequence similarity to an NADH oxidase and a CBS-domain-containing protein, respectively, but their deletion did not affect resistance to arsenite or arsenate in E. coli. TnAtcArs was actively transposed in E. coli, but no increase in transposition frequency in the presence of arsenic was detected. Northern hybridization and reporter gene studies indicated that although ArsR regulated the 10 kb operon containing the arsenic-resistance genes in response to arsenic, ArsR had no effect on the regulation of genes associated with transposition activity.

Efficient transformation of sucrose into high pullulan concentrations by Aureobasidium melanogenum TN1-2 isolated from a natural honey.

PubMed

Jiang, Hong; Xue, Si-Jia; Li, Yan-Feng; Liu, Guang-Lei; Chi, Zhen-Ming; Hu, Zhong; Chi, Zhe

2018-08-15

A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor. The Mw of the produced pullulan was 1.42 × 10 5  g/mol. The low Mw may be due to the high α-amylase, glucoamylase and isopullulanase activities. The intracellular level of trehalose had no influence on high pullulan production by the yeast-like fungal strain TN1-2. Copyright © 2018. Published by Elsevier Ltd.

Predictors of Youths' Posttraumatic Stress Symptoms Following a Natural Disaster: The 2010 Nashville, Tennessee, Flood.

PubMed

Martin, Nina C; Felton, Julia W; Cole, David A

2016-01-01

Framed by a previously established conceptual model of youths' posttraumatic stress (PTS) responses following a disaster, the current longitudinal study examined the relation of predisaster child characteristics (age, gender, depressive symptoms, ruminative coping), predisaster environmental characteristics (negative life events and supportive and negative friendship interactions), and level of disaster exposure to youths' PTS symptoms in the wake of a natural disaster. Prior to the 2010 Nashville, Tennessee, flood, 239 predominantly Caucasian youth from four elementary and middle schools (ages = 10-15, 56% girls) completed measures of depressive symptoms, rumination, negative life events, and social support in the form of both supportive and negative friendship interactions. Approximately 10 days after returning to school, 125 completed measures of disaster exposure and postflood PTS symptoms. Bivariate correlations revealed that disaster-related PTS symptoms were unrelated to age, gender, or predisaster supportive friendship interactions and significantly positively related to level of disaster exposure and predisaster levels of negative life events, depressive symptoms, rumination, and negative friendship interactions. After controlling for level of disaster exposure and other predisaster child and environmental characteristics, depressive symptoms and negative friendship interactions predicted postdisaster PTS symptoms. The effect of child's flood-related experiences on PTS symptoms was not moderated by any of the preexisting child characteristics or environmental indicators. Faced with limited resources after a natural disaster, school counselors and other health professionals should focus special attention on youths who experienced high levels of disaster-related losses and whose predisaster emotional and interpersonal lives were problematic.

Do reclamation speed up recovery of soil and soil biota on post mining sites along climatic gradient across continental USA?

NASA Astrophysics Data System (ADS)

Frouz, Jan

2015-04-01

Soil biota community (macrofauna, nematodes and microbial community studied by PLFA) was studied together with soil development in post-mining chronosequences along climatic gradient in the USA, covering hardwood forest (TN, IN), tallgrass prairie (IL), or shortgrass prairie (WY). Post mining sites reclaimed according recent regulation which includes topsoil application and vegetation establishment were compared to local climax. Both young and old restoration sites were much closer to the climax condition in shortgrass prairie than in the other sites. The shortgrass prairie soil community contained abundant root-feeding organisms, which may establish quicker than the saprophagous fauna that was abundant at the other sites. Absence of saprophagous groups, and especially earthworms, resulted in the absence of bioturbation in shortgrass prairie sites while in chronosequences other than the one in shortgrass prairie, bioturbation played an important role in topsoil formation resulting in more complex soil profile development compare to shortgrass prairie. This may contribute to faster recovery communities in shortgrass prairie in comparison with tallgrass prairie and forest as At the same time sites that were reclaimed according recent regulation (topsoil application and vegetation establishment) were compare to unreclaimed sites both about 30 years old in TN IL and WY. It TN soil and soil biota seems to approach fasted to climax in unreclaimed than reclaimed sites. In IL this differences between reclaimed and unreclaimed sites was not so clear. While in WY reclaimed sites seems to approach to climax community fasted than unreclaimed one. This suggests that effect of reclamation vary along climatic gradient. In drier sites, formation of soil matrix from parent material is probably much slower and topsoil application speed up soil community recovery substantially while this effect is less pronounces in more wet sites, where soil compaction due to restoration may in some

77 FR 15995 - Foreign-Trade Zone 148-Knoxville, TN; Application for Reorganization Under Alternative Site...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-03-19

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 16-2012] Foreign-Trade Zone 148--Knoxville, TN; Application for Reorganization Under Alternative Site Framework An application has been... and adjacent to the Knoxville U.S. Customs and Border Protection port of entry. The applicant is...

pp-GalNAc-T13 induces high metastatic potential of murine Lewis lung cancer by generating trimeric Tn antigen

DOE Office of Scientific and Technical Information (OSTI.GOV)

Matsumoto, Yasuyuki; Zhang, Qing; Akita, Kaoru

2012-03-02

Highlights: Black-Right-Pointing-Pointer ppGalNAc-T13 was up-regulated in high metastatic sublines of Lewis lung cancer. Black-Right-Pointing-Pointer ppGalNAc-T13 expression enhanced cell invasion activity in low metastatic sublines. Black-Right-Pointing-Pointer Trimeric Tn antigen was induced in the transfectant cells of ppGalNAc-T13 cDNA. Black-Right-Pointing-Pointer A major protein carrying trimeric Tn structure was identified as Syndecan-1. Black-Right-Pointing-Pointer Silencing of ppGalNAc-T13 resulted in the reduction of invasion and of metastasis.. -- Abstract: In order to analyze the mechanisms for cancer metastasis, high metastatic sublines (H7-A, H7-Lu, H7-O, C4-sc, and C4-ly) were obtained by repeated injection of mouse Lewis lung cancer sublines H7 and C4 into C57BL/6 mice. Thesemore » sublines exhibited increased proliferation and invasion activity in vitro. Ganglioside profiles exhibited lower expression of GM1 in high metastatic sublines than the parent lines. Then, we established GM1-Si-1 and GM1-Si-2 by stable silencing of GM1 synthase in H7 cells. These GM1-knockdown clones exhibited increased proliferation and invasion. Then, we explored genes that markedly altered in the expression levels by DNA microarray in the combination of C4 vs. C4-ly or H7 vs. H7 (GM1-Si). Consequently, pp-GalNAc-T13 gene was identified as up-regulated genes in the high metastatic sublines. Stable transfection of pp-GalNAc-T13 cDNA into C4 (T13-TF) resulted in increased invasion and motility. Then, immunoblotting and flow cytometry using various antibodies and lectins were performed. Only anti-trimeric Tn antibody (mAb MLS128), showed increased expression levels of trimeric Tn antigen in T13-TF clones. Moreover, immunoprecipitation/immunoblotting was performed by mAb MLS128, leading to the identification of an 80 kDa band carrying trimeric Tn antigen, i.e. Syndecan-1. Stable silencing of endogenous pp-GalNAc-T13 in C4-sc (T13-KD) revealed that primary tumors generated

Tn5253 family integrative and conjugative elements carrying mef(I) and catQ determinants in Streptococcus pneumoniae and Streptococcus pyogenes.

PubMed

Mingoia, Marina; Morici, Eleonora; Morroni, Gianluca; Giovanetti, Eleonora; Del Grosso, Maria; Pantosti, Annalisa; Varaldo, Pietro E

2014-10-01

The linkage between the macrolide efflux gene mef(I) and the chloramphenicol inactivation gene catQ was first described in Streptococcus pneumoniae (strain Spn529), where the two genes are located in a module designated IQ element. Subsequently, two different defective IQ elements were detected in Streptococcus pyogenes (strains Spy029 and Spy005). The genetic elements carrying the three IQ elements were characterized, and all were found to be Tn5253 family integrative and conjugative elements (ICEs). The ICE from S. pneumoniae (ICESpn529IQ) was sequenced, whereas the ICEs from S. pyogenes (ICESpy029IQ and ICESpy005IQ, the first Tn5253-like ICEs reported in this species) were characterized by PCR mapping, partial sequencing, and restriction analysis. ICESpn529IQ and ICESpy029IQ were found to share the intSp 23FST81 integrase gene and an identical Tn916 fragment, whereas ICESpy005IQ has int5252 and lacks Tn916. All three ICEs were found to lack the linearized pC194 plasmid that is usually associated with Tn5253-like ICEs, and all displayed a single copy of a toxin-antitoxin operon that is typically contained in the direct repeats flanking the excisable pC194 region when this region is present. Two different insertion sites of the IQ elements were detected, one in ICESpn529IQ and ICESpy029IQ, and another in ICESpy005IQ. The chromosomal integration of the three ICEs was site specific, depending on the integrase (intSp 23FST81 or int5252). Only ICESpy005IQ was excised in circular form and transferred by conjugation. By transformation, mef(I) and catQ were cotransferred at a high frequency from S. pyogenes Spy005 and at very low frequencies from S. pneumoniae Spn529 and S. pyogenes Spy029. Copyright © 2014, American Society for Microbiology. All Rights Reserved.

The History of One-Hundred Thirteen P-38 Lightning Aircraft Constructed by Consolidated-Vultee Aviation Corporation of Nashville Tennessee, 1945

NASA Astrophysics Data System (ADS)

Bitzer, George

The purpose of this research project was to trace and locate 113 P- 38 Lightning Aircraft that were constructed by Consolidated-Vultee Aviation Corporation of Nashville-Tennessee. A determination as to where they were located at the end of World War II, along with their subsequent location, was attempted. This project utilized historical research with a qualitative approach where data was accumulated, evaluated and formulated based on events that happened 70 years ago. A triangulation practice is the process of collecting data, not from just one source, but from several sources. This process helps strengthen the individual data, compensated by others to create a complete picture of the phenomenon being researched. The research does not focus on pilots that flew the P-38's. However, a few pilot names may be mentioned that were involved in an accident the aircraft may have suffered. The Army Air Force requested 2000 P-38's Model " L " built by Consolidated-Vultee for the Lockheed Aircraft Corporation in Burbank, California. The War Department did not know at the time how long the present conflict of war might last.

The L‐type Ca2+ channel facilitates abnormal metabolic activity in the cTnI‐G203S mouse model of hypertrophic cardiomyopathy

PubMed Central

Viola, Helena; Johnstone, Victoria; Cserne Szappanos, Henrietta; Richman, Tara; Tsoutsman, Tatiana; Filipovska, Aleksandra; Semsarian, Christopher

2016-01-01

Key points Genetic mutations in cardiac troponin I (cTnI) are associated with development of hypertrophic cardiomyopathy characterized by myocyte remodelling, disorganization of cytoskeletal proteins and altered energy metabolism.The L‐type Ca2+ channel is the main route for calcium influx and is crucial to cardiac excitation and contraction. The channel also regulates mitochondrial function in the heart by a functional communication between the channel and mitochondria via the cytoskeletal network.We find that L‐type Ca2+ channel kinetics are altered in cTnI‐G203S cardiac myocytes and that activation of the channel causes a significantly greater increase in mitochondrial membrane potential and metabolic activity in cTnI‐G203S cardiac myocytes.These responses occur as a result of impaired communication between the L‐type Ca2+ channel and cytoskeletal protein F‐actin, involving decreased movement of actin–myosin and block of the mitochondrial voltage‐dependent anion channel, resulting in a ‘hypermetabolic’ mitochondrial state.We propose that L‐type Ca2+ channel antagonists, such as diltiazem, might be effective in reducing the cardiomyopathy by normalizing mitochondrial metabolic activity. Abstract Genetic mutations in cardiac troponin I (cTnI) account for 5% of families with hypertrophic cardiomyopathy. Hypertrophic cardiomyopathy is associated with disorganization of cytoskeletal proteins and altered energy metabolism. The L‐type Ca2+ channel (ICa‐L) plays an important role in regulating mitochondrial function. This involves a functional communication between the channel and mitochondria via the cytoskeletal network. We investigate the role of ICa‐L in regulating mitochondrial function in 25‐ to 30‐week‐old cardiomyopathic mice expressing the human disease‐causing mutation Gly203Ser in cTnI (cTnI‐G203S). The inactivation rate of ICa‐L is significantly faster in cTnI‐G203S myocytes [cTnI‐G203S: τ1 = 40.68 ± 3.22, n

Drivers of long-term precipitation and runoff variability in the southeastern USA

NASA Astrophysics Data System (ADS)

Engström, Johanna; Waylen, Peter

2018-02-01

The hydroclimatology of the southeastern USA (AL, GA, NC, SC, and TN) is analyzed from a holistic perspective, including multiple climate drivers. Monthly precipitation modeled by the PRISM group and runoff data (1952-2011) from 18 basins are analyzed using a single-field based principal component's analysis. Results indicate that the Atlantic Multidecadal Oscillation and El Niño-Southern Oscillation are the main atmospheric drivers of hydroclimate variability in the region, sometimes operating at several months' lag. Their influence is the strongest in the fall through spring, which corresponds with the dry season in the southern parts of the study area thereby increasing pressure on already limited water resources. The Arctic Oscillation, North Atlantic Oscillation, and Pacific-North American patterns vary on shorter-term bases, and also show a significant, but temporally more sporadic influence. Insight is also brought to the ongoing discussion, confirming the disassociation of the Arctic and North Atlantic Oscillation. Findings can be used in water resources forecasting, giving an indication of expected water volumes several months ahead.

On the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_1^\\alpha ({T^N})

NASA Astrophysics Data System (ADS)

Ahmedov, Anvarjon; Materneh, Ehab; Zainuddin, Hishamuddin

2017-09-01

The relevance of waves in quantum mechanics naturally implies that the decomposition of arbitrary wave packets in terms of monochromatic waves plays an important role in applications of the theory. When eigenfunction expansions does not converge, then the expansions of the functions with certain smoothness should be considered. Such functions gained prominence primarily through their application in quantum mechanics. In this work we study the almost everywhere convergence of the eigenfunction expansions from Liouville classes L_p^α ({T^N}), related to the self-adjoint extension of the Laplace operator in torus TN . The sufficient conditions for summability is obtained using the modified Poisson formula. Isomorphism properties of the elliptic differential operators is applied in order to obtain estimation for the Fourier series of the functions from the classes of Liouville L_p^α .

75 FR 28302 - American Food and Vending Spring Hill, TN; Notice of Negative Determination Regarding Application...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-20

... Spring Hill, TN; Notice of Negative Determination Regarding Application for Reconsideration By... negative determination applicable to workers and former workers at American Food and Vending, Spring Hill... eligible for TAA because they are service workers who provided services to General Motors, Spring Hill...

75 FR 74002 - Foreign-Trade Zone 148-Knoxville, TN, Application for Subzone Toho, Tenax America, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-11-30

... DEPARTMENT OF COMMERCE Foreign-Trade Zones Board [Docket 57-2010] Foreign-Trade Zone 148--Knoxville, TN, Application for Subzone Toho, Tenax America, Inc., Extension of Comment Period The comment period for the application for subzone status at the Toho Tenax America, Inc., facility in Rockwood...

A TALE of transposition: Tn3-like transposons play a major role in the spread of pathogenicity determinants of Xanthomonas citri and other xanthomonads.

PubMed

Ferreira, Rafael Marini; de Oliveira, Amanda Carolina P; Moreira, Leandro M; Belasque, José; Gourbeyre, Edith; Siguier, Patricia; Ferro, Maria Inês T; Ferro, Jesus A; Chandler, Michael; Varani, Alessandro M

2015-02-17

Members of the genus Xanthomonas are among the most important phytopathogens. A key feature of Xanthomonas pathogenesis is the translocation of type III secretion system (T3SS) effector proteins (T3SEs) into the plant target cells via a T3SS. Several T3SEs and a murein lytic transglycosylase gene (mlt, required for citrus canker symptoms) are found associated with three transposition-related genes in Xanthomonas citri plasmid pXAC64. These are flanked by short inverted repeats (IRs). The region was identified as a transposon, TnXax1, with typical Tn3 family features, including a transposase and two recombination genes. Two 14-bp palindromic sequences within a 193-bp potential resolution site occur between the recombination genes. Additional derivatives carrying different T3SEs and other passenger genes occur in different Xanthomonas species. The T3SEs include transcription activator-like effectors (TALEs). Certain TALEs are flanked by the same IRs as found in TnXax1 to form mobile insertion cassettes (MICs), suggesting that they may be transmitted horizontally. A significant number of MICs carrying other passenger genes (including a number of TALE genes) were also identified, flanked by the same TnXax1 IRs and delimited by 5-bp target site duplications. We conclude that a large fraction of T3SEs, including individual TALEs and potential pathogenicity determinants, have spread by transposition and that TnXax1, which exhibits all of the essential characteristics of a functional transposon, may be involved in driving MIC transposition. We also propose that TALE genes may diversify by fork slippage during the replicative Tn3 family transposition. These mechanisms may play a crucial role in the emergence of Xanthomonas pathogenicity. Xanthomonas genomes carry many insertion sequences (IS) and transposons, which play an important role in their evolution and architecture. This study reveals a key relationship between transposons and pathogenicity determinants in

Contribution of biogenic emissions to the formation of ozone and particulate matter in the eastern United States.

PubMed

Pun, Betty K; Wu, Shiang-Yuh; Seigneur, Christian

2002-08-15

As anthropogenic emissions of ozone (O3) precursors, fine particulate matter (PM2.5), and PM2.5 precursors continue to decrease in the United States, the fraction of O3 and PM2.5 attributable to natural sources may become significant in some locations, reducing the efficacy that can be expected from future controls of anthropogenic sources. Modeling studies were conducted to estimate the contribution of biogenic emissions to the formation of O3 and PM2.5 in Nashville/TN and the northeastern United States. Two approaches were used to bound the estimates. In an anthropogenic simulation, biogenic emissions and their influence at the domain boundaries were eliminated. Contributions of biogenic compounds to the simulated concentrations of O3 and PM2.5 were determined by the deviation of the concentrations in the anthropogenic case from those in the base case. A biogenic simulation was used to assess the amounts of O3 and PM2.5 produced in an environment free from anthropogenic influences in emissions and boundary conditions. In both locations, the contribution of biogenic emissions to O3 was small (<23%) on a domain-wide basis, despite significant biogenic volatile organic compounds (VOC) emissions (65-89% of total VOC emissions). However, the production of O3 was much more sensitive to biogenic emissions in urban areas (22-34%). Therefore, the effects of biogenic emissions on O3 manifested mostly via their interaction with anthropogenic emissions of NOx. In the anthropogenic simulations, the average contribution of biogenic and natural sources to PM2.5 was estimated at 9% in Nashville/TN and 12% in the northeast domain. Because of the long atmospheric lifetimes of PM2.5, the contribution of biogenic/natural PM2.5 from the boundary conditions was higher than the contribution of biogenic aerosols produced within the domain. The elimination of biogenic emissions also affected the chemistry of other secondary PM2.5 components. Very little PM2.5 was formed in the biogenic

Asia-Pacific consensus statement on the optimal use of high-sensitivity troponin assays in acute coronary syndromes diagnosis: focus on hs-TnI

PubMed Central

Tan, Jack Wei Chieh; Lam, Carolyn S P; Kasim, Sazzli Shahlan; Aw, Tar Choon; Abanilla, Joel M; Chang, Wei-Ting; Dang, Van Phuoc; Iboleon-Dy, Maria; Mumpuni, Sari Sri; Phommintikul, Arintaya; Ta, Manh Cuong; Topipat, Punkiat; Yiu, Kai Hang; Cullen, Louise

2017-01-01

Objective High-sensitivity troponin (hs-Tn) assays need to be applied appropriately to improve diagnosis and patient outcomes in acute coronary syndromes (ACS). Methods Experts from Asia Pacific convened in 2015 to provide data-driven consensus-based, region-specific recommendations and develop an algorithm for the appropriate incorporation of this assay into the ACS assessment and treatment pathway. Results Nine recommendations were developed by the expert panel: (1) troponin is the preferred cardiac biomarker for diagnostic assessment of ACS and is indicated for patients with symptoms of possible ACS; (2) hs-Tn assays are recommended; (3) serial testing is required for all patients; (4) testing should be performed at presentation and 3 hours later; (5) gender-specific cut-off values should be used for hs-Tn I assays; (6) hs-Tn I level >10 times the upper limit of normal should be considered to ‘rule in’ a diagnosis of ACS; (7) dynamic change >50% in hs-Tn I level from presentation to 3-hour retest identifies patients at high risk for ACS; (8) where only point-of-care testing is available, patients with elevated readings should be considered at high risk, while patients with low/undetectable readings should be retested after 6 hours or sent for laboratory testing and (9) regular education on the appropriate use of troponin tests is essential. Conclusions We propose an algorithm that will potentially reduce delays in discharge by the accurate ‘rule out’ of non-ACS patients within 3 hours. Appropriate research should be undertaken to ensure the efficacy and safety of the algorithm in clinical practice, with the long-term goal of improvement of care of patients with ACS in Asia Pacific. PMID:28466882

Heart-type fatty acid-binding protein (H-FABP) and highly sensitive troponin T (hsTnT) as markers of myocardial injury and cardiovascular events in elective percutaneous coronary intervention (PCI).

PubMed

Connolly, M; Shand, J; Kinnin, M; Menown, I; Kurth, M J; Lamont, J; Mc Eneaney, D

2018-01-01

Type 4a myocardial infarction (MI) occurs when myocardial injury is combined with either symptoms suggestive of myocardial ischaemia, new left bundle branch block, angiographic loss of patency of a major artery or imaging suggestive of new loss of myocardium. Myocardial injury is defined as a rise of >5 x 99th upper reference limit (URL) of 14 ng/l (i.e. >70 ng/l) for highly sensitive troponin T (hsTnT) at 6 h if hsTnT was normal at baseline or >20% rise from 0 to 6 h if hsTnT was >14 ng/l at baseline. To assess the prognostic value of biomarkers of myocardial injury following elective percutaneous coronary intervention (PCI). A cohort of 209 patients were included of whom 144 (68.9%) were male, mean age was 68.8 years, 28 (13.4%) were smokers, 31 (14.8%) were diabetic, 199 (95.2%) had hypercholesterolaemia and 138 (66.0%) had hypertension. We evaluated hsTnT, heart-type fatty acid-binding protein (H-FABP), troponin I (TnI), creatine kinase MB type (CKMB), myoglobin, glycogen phosphorylase BB (GPBB) and carbonic anhydrase III (CA III) at 0, 4, 6 and 24 h following elective PCI. Patients were followed up at 1 year to assess for major adverse clinical events (MACE). Myocardial injury was observed in 37 (17.7%) patients. Median hsTnT/H-FABP at 4 h were most predictive. MACE was noted in 6 (2.9%) patients, 3 had type 4a MI post PCI, P = 0.036. Median 4 h hsTnT/H-FABP were most predictive of myocardial injury following PCI. H-FABP and hsTnT were predictive of MACE. © The Author 2017. Published by Oxford University Press on behalf of the Association of Physicians. All rights reserved. For Permissions, please email: journals.permissions@oup.com

'Test n Treat (TnT)'- Rapid testing and same-day, on-site treatment to reduce rates of chlamydia in sexually active further education college students: study protocol for a cluster randomised feasibility trial.

PubMed

Kerry-Barnard, Sarah; Fleming, Charlotte; Reid, Fiona; Phillips, Rachel; Drennan, Vari M; Adams, Elisabeth J; Majewska, Wendy; Balendra, Anjella; Harding-Esch, Emma; Cousins, Emma; Tariq Sadiq, S; Oakeshott, Pippa

2018-06-05

Sexually active young people attending London further education (FE) colleges have high rates of chlamydia, but screening rates are low. We will conduct a cluster randomised feasibility trial of frequent, rapid, on-site chlamydia testing and same-day treatment (Test and Treat (TnT)) in six FE colleges (with parallel qualitative and economic assessments) to assess the feasibility of conducting a future trial to investigate if TnT reduces chlamydia rates. We will recruit 80 sexually active students aged 16-24 years from public areas at each of six colleges. All participants (total n = 480) will be asked to provide samples (urine for males, self-taken vaginal swabs for females) and complete questionnaires on sexual lifestyle and healthcare use at baseline and after 7 months. Participants will be informed that baseline samples will not be tested for 7 months and be advised to get screened separately. Colleges will be randomly allocated to the intervention (TnT) or the control group (no TnT). One and 4 months after recruitment, participants at each intervention college (n = 3) will be texted and invited for on-site chlamydia tests using the 90-min Cepheid GeneXpert system. Students with positive results will be asked to see a visiting nurse health adviser for same-day treatment and partner notification, (backed by genitourinary medicine follow-up). Participants in control colleges (n = 3) will receive 'thank you' texts 1 and 4 months after recruitment. Seven months after recruitment, participants from both groups will be invited to complete questionnaires and provide samples for TnT. All samples will be tested, and same-day treatment offered to students with positive results. Acceptability of TnT will be assessed by qualitative interviews of purposively sampled students (n = 30) and college staff (n = 12). We will collect data on costs of TnT and usual healthcare. Findings will provide key values to inform feasibility, sample size and timescales of a

Anal Carcinoma: Impact of TN Category of Disease on Survival, Disease Relapse, and Colostomy Failure in US Gastrointestinal Intergroup RTOG 98-11 Phase 3 Trial

PubMed Central

Gunderson, Leonard L.; Moughan, Jennifer; Ajani, Jaffer A.; Pedersen, John E.; Winter, Kathryn A.; Benson, Al B.; Thomas, Charles R.; Mayer, Robert J.; Haddock, Michael G.; Rich, Tyvin A.; Willett, Christopher G.

2013-01-01

Purpose The long-term update of US GI Intergroup RTOG 98-11 anal cancer trial found that concurrent chemoradiation (CCRT) with fluorouracil (5-FU) plus mitomycin had a significant impact on disease-free survival (DFS) and overall survival (OS) compared with induction plus concurrent 5-FU plus cisplatin. The intent of the current analysis was to determine the impact of tumor node (TN) category of disease on survival (DFS and OS), colostomy failure (CF), and relapse (local-regional failure [LRF] and distant metastases [DM]) in this patient group. Methods and Materials DFS and OS were estimated univariately by using the Kaplan-Meier method, and 6 TN categories were compared by the log–rank test (T2N0, T3N0, T4N0, T2N1-3, T3N1-3, and T4N1-3). Time to relapse and colostomy were estimated by the cumulative incidence method, and TN categories were compared using Gray’s test. Results Of 682 patients, 620 were analyzable for outcomes by TN category. All endpoints showed statistically significant differences among the TN categories of disease (OS, P<.0001; DFS, P<.0001; LRF, P<.0001; DM, P=.0011; CF, P=.01). Patients with the poorest OS, DFS, and LRF outcomes were those with T3-4N-positive (+) disease. CF was lowest for T2N0 and T2N+ (11%, 11%, respectively) and worst for the T4N0, T3N+, and T4N+ categories (26%, 27%, 24%, respectively). Conclusions TN category of disease has a statistically significant impact on OS, DFS, LRF, DM, and CF in patients treated with CCRT and provides excellent prognostic information for outcomes in patients with anal carcinoma. Significant challenges remain for patients with T4N0 and T3-4N+ categories of disease with regard to survival, relapse, and CF and lesser challenges for T2-3N0/T2N+ categories. PMID:24035327

77 FR 73978 - Foreign-Trade Zone 148-Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-12

...--Knoxville, TN, Toho Tenax America, Inc. (Carbon Fiber Manufacturing Authority), Opening of Comment Period on... manufacture carbon fiber for export and oxidized polyacrylonitrile fiber (Board Order 1868, 77 FR 69435, 11/19/2012). Board Order 1868 did not include authority to manufacture carbon fiber for the U.S. market; the...

75 FR 61696 - Foreign-Trade Zone 148-Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-06

...--Knoxville, TN; Application for Subzone; Toho Tenax America, Inc. (Carbon Fiber and Oxidized..., requesting special-purpose subzone status for the carbon fiber and oxidized polyacrylonitrile fiber (OPF...)--based carbon fiber and OPF (up to 4,000 metric tons combined annually) for export and the domestic...

Generation and validation of oxygenated volatile organic carbon standards for the 1995 Southern Oxidants Study Nashville Intensive

NASA Astrophysics Data System (ADS)

Apel, E. C.; Calvert, J. G.; Greenberg, J. P.; Riemer, D.; Zika, R.; Kleindienst, T. E.; Lonneman, W. A.; Fung, K.; Fujita, E.

1998-09-01

Two volatile organic compound (VOC) mixtures were made available and utilized for the calibration of instruments and intercomparison exercises at the Youth, Inc. (YI) site during the Southern Oxidants Study Nashville Intensive. Cylinder 1, made by Scott-Marrin, Inc., contained 14 components (3 nonmethane hydrocarbons (NMHCs) and 11 oxygen-containing VOCs (OVOCs)) and is referred to as OVOCl. Cylinder 2, made at the National Center for Atmospheric Research (NCAR), contained 4 components (one NMHC and three OVOCs) and is referred to as OVOC2. The mixtures were not prepared by either laboratory as primary standards but significant effort was applied to validate their concentrations by several different laboratories using several different techniques. The mixtures were prepared in high-pressure cylinders in the parts per million by volume (ppmv) range using calibrated syringe methods. Gas Chromatographic (GC) and 2,4-dinitrophenylhydrazine (DNPH) cartridge/high-pressure liquid Chromatographic (HPLC) systems were utilized in the calibration of the cylinders. GC detectors included the flame ionization detector (FID) and atomic emission detector (AED). Results obtained with the GC/FID systems for OVOCs were adjusted in terms of the effective carbon number (ECN), obtained from literature values, to correct for the reduced response of the FID for compounds containing oxygen, relative to compounds containing only carbon and hydrogen. Cylinder concentrations were derived and compared based on the ECN-adjusted FID results, the AED results, and the DNPH cartridge results. The various methods employed agreed to within approximately 15%. Both cylinders were stable (±4%) over a period of 2 years.

Troponin C Mutations Partially Stabilize the Active State of Regulated Actin and Fully Stabilize the Active State When Paired with Δ14 TnT.

PubMed

Baxley, Tamatha; Johnson, Dylan; Pinto, Jose R; Chalovich, Joseph M

2017-06-13

Striated muscle contraction is regulated by the actin-associated proteins tropomyosin and troponin. The extent of activation of myosin ATPase activity is lowest in the absence of both Ca 2+ and activating cross-bridges (i.e., S1-ADP or rigor S1). Binding of activating species of myosin to actin at a saturating Ca 2+ concentration stabilizes the most active state (M state) of the actin-tropomyosin-troponin complex (regulated actin). Ca 2+ binding alone produces partial stabilization of the active state. The extent of stabilization at a saturating Ca 2+ concentration depends on the isoform of the troponin subunits, the phosphorylation state of troponin, and, in the case of cardiac muscle, the presence of hypertrophic cardiomyopathy-producing mutants of troponin T and troponin I. Cardiac dysfunction is also associated with mutations of troponin C (TnC). Troponin C mutants A8V, C84Y, and D145E increase the Ca 2+ sensitivity of ATPase activity. We show that these mutants change the distribution of regulated actin states. The A8V and C84Y TnC mutants decreased the inactive B state distribution slightly at low Ca 2+ concentrations, but the D145E mutants had no effect on that state. All TnC mutants increased the level of the active M state compared to that of the wild type, at a saturating Ca 2+ concentration. Troponin complexes that contained two mutations that stabilize the active M state, A8V TnC and Δ14 TnT, appeared to be completely in the active state in the presence of only Ca 2+ . Because Ca 2+ gives full activation, in this situation, troponin must be capable of positioning tropomyosin in the active M state without the need for rigor myosin binding.

Abrasive wear of Hilong BoTN hardfacings

NASA Astrophysics Data System (ADS)

Fedorova, L.; Fedorov, S.; Sadovnikov, A.; Ivanova, Y.; Voronina, M.

2018-02-01

The spread of steels, which are used to produce locks of steel drill pipes, adversely affects their wear resistance, which, in combination with low hardness of HV 2400 ... 2800 MPa as well as of the thread of screw, results in low wear resistance and the need for their reconstruction at the pipe control shop. An efficient way of improving the quality of drill pipe jonts is to hard-face them by the outside diameter with wear-resistant materials (hardbanding). One of the companies engaged in the development of hardfacing materials and hardbanding is Hilong (China) with weld seams of the brand BoTn. According to the results of the studies the following conclusion can be made: hardfacing increases the durability of the hardware, contributing to an increase in wear resistance of locks of DP under the conditions of abrasive action of aggressive geological formations; the usage of DP without wear-resistant weld seams is impermissible, because their further operation, as part of the drill-stem, can lead to emergency consequences; application of the pipes with the hardfacing collars together with the collars without hardfacing, due to varying degree of wear of jonts in the drill-stem, is also impermissible.

Interdisciplinary Dialogue for Education, Collaboration, and Innovation: Intelligent Biology and Medicine In and Beyond 2013

DOE Office of Scientific and Technical Information (OSTI.GOV)

Zhang, Bing; Huang, Yufei; McDermott, Jason E.

The 2013 International Conference on Intelligent Biology and Medicine (ICIBM 2013) was held on August 11-13, 2013 in Nashville, Tennessee, USA. The conference included six scientific sessions, two tutorial sessions, one workshop, two poster sessions, and four keynote presentations that covered cutting-edge research topics in bioinformatics, systems biology, computational medicine, and intelligent computing. Here, we present a summary of the conference and an editorial report of the supplements to BMC Genomics and BMC Systems Biology that include 19 research papers selected from ICIBM 2013.

78 FR 54168 - Special Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN

Federal Register 2010, 2011, 2012, 2013, 2014

2013-09-03

... local regulation will not have a significant economic impact on a substantial number of small entities... Local Regulation, Cumberland River, Mile 157.0 to 159.0; Ashland City, TN AGENCY: Coast Guard, DHS. ACTION: Temporary final rule. SUMMARY: The Coast Guard is establishing a temporary special local...

Implications for future activity of Grímsvötn volcano, Iceland, from compositional time series of historical tephra

NASA Astrophysics Data System (ADS)

Carpentier, Marion; Sigmarsson, Olgeir; Larsen, Gudrun

2014-05-01

The nature of future eruptions of active volcanoes is hard to predict. Improved understanding of the past volcanic activity is probably the best way to infer future eruptive scenarios. The most active volcano in Iceland, Grímsvötn, last erupted in 2011 with consequences for habitants living close to the volcano and aviation in the North-Atlantic. In an effort to better understand the magmatic system of the volcano, we have investigated the compositions of 23 selected tephra layers representing the last 8 centuries of volcanic activity at Grímsvötn. The tephra was collected in the ablation area of outlet glaciers from Vatnajökull ice cap. The ice-conserved tephra are less prone to alteration than those exposed in soil sections. Major element analyses are indistinguishable and of quartz-normative tholeiite composition, and Sr and Nd isotope ratios are constant. In contrast, both trace element concentrations (Th range from 0.875 ppm to 1.37 ppm and Ni from 28.5 ppm to 56.6 ppm) in the basalts and Pb isotopes show small but significant variations. The high-precision analyses of Pb isotope ratios allow the identification of tephra samples (3 in total) with more radiogenic ratios than the bulk of the samples. The tephra of constant isotope ratios show linear increase in incompatible element concentrations with time. The rate of increasing concentrations permits exploring possible future scenarios assuming that the magmatic system beneath the volcano follows the established historical evolution. Assuming similar future behaviour of the magma system beneath Grímsvötn volcano, the linear increase in e.g. Th concentration suggests that the volcano is likely to principally produce basalts for the next 500-1000 years. Evolution of water concentration will most likely follow those of incompatible elements with consequent increases in explosiveness of future Grímsvötn eruptions.

TnSeq of Mycobacterium tuberculosis clinical isolates reveals strain-specific antibiotic liabilities

PubMed Central

Carey, Allison F.; Rock, Jeremy M.; Krieger, Inna V.; Gagneux, Sebastien; Sacchettini, James C.; Fortune, Sarah M.

2018-01-01

Once considered a phenotypically monomorphic bacterium, there is a growing body of work demonstrating heterogeneity among Mycobacterium tuberculosis (Mtb) strains in clinically relevant characteristics, including virulence and response to antibiotics. However, the genetic and molecular basis for most phenotypic differences among Mtb strains remains unknown. To investigate the basis of strain variation in Mtb, we performed genome-wide transposon mutagenesis coupled with next-generation sequencing (TnSeq) for a panel of Mtb clinical isolates and the reference strain H37Rv to compare genetic requirements for in vitro growth across these strains. We developed an analytic approach to identify quantitative differences in genetic requirements between these genetically diverse strains, which vary in genomic structure and gene content. Using this methodology, we found differences between strains in their requirements for genes involved in fundamental cellular processes, including redox homeostasis and central carbon metabolism. Among the genes with differential requirements were katG, which encodes the activator of the first-line antitubercular agent isoniazid, and glcB, which encodes malate synthase, the target of a novel small-molecule inhibitor. Differences among strains in their requirement for katG and glcB predicted differences in their response to these antimicrobial agents. Importantly, these strain-specific differences in antibiotic response could not be predicted by genetic variants identified through whole genome sequencing or by gene expression analysis. Our results provide novel insight into the basis of variation among Mtb strains and demonstrate that TnSeq is a scalable method to predict clinically important phenotypic differences among Mtb strains. PMID:29505613

EnviroAtlas -- Memphis, TN (2012) -- One Meter Resolution Urban Land Cover Data

EPA Pesticide Factsheets

The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These leaf-on LC data and maps were derived from 1-m pixel, four-band (red, green, blue, and near-infrared) aerial photography acquired from the United States Department of Agriculture (USDA) National Agriculture Imagery Program (NAIP) on four dates in 2012: June 15, June 18, June 21 and June 23, and one date in 2013: July 12. Three separate LiDAR (Light Detection and Ranging) data sets collected on February 19, 2009 00e2?? August 2, 2010, December 1-2, 2011 and January 23-24, 2012 were integrated for Shelby Co., TN, Crittenden Co., AR, and DeSoto Co, MS. Five MULC classes were mapped directly from the NAIP and LiDAR data: Water, Impervious, Soil, Trees, and Grass/Herbaceous. Agriculture was derived from USDA Common Land Unit (CLU) data. Woody and emergent wetlands were copied from existing National Wetlands Inventory (NWI) data. Analysis of a random sampling of 612 photo-interpreted land cover reference points yielded an overall users accuracy of 86.9%. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-use, mapping application to view and analyze multiple ecosystem services for the contiguous United States. The dataset is available as do

A role for Tn6029 in the evolution of the complex antibiotic resistance gene loci in genomic island 3 in enteroaggregative hemorrhagic Escherichia coli O104:H4.

PubMed

Roy Chowdhury, Piklu; Charles, Ian G; Djordjevic, Steven P

2015-01-01

In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in the region of divergence 1 (RD1) within E. coli genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding resistance to ampicillin, streptomycin, sulfamethoxazole, tetracycline and trimethoprim respectively. The precise arrangement of antibiotic resistance genes and the role of mobile elements that drove the evolutionary events and created the CRL have not been investigated. We used a combination of bioinformatics and iterative BLASTn searches to determine the micro-evolutionary events that likely led to the formation of the CRL in GI3 using the closed genome sequences of EAHEC O104:H4 strains 2011C-3493 and 2009EL-2050 and high quality draft genomes of EAHEC E. coli O104:H4 isolates from sporadic cases not associated with the initial outbreak. Our analyses indicate that the CRL in GI3 evolved from a progenitor structure that contained an In2-derived class 1 integron in a Tn21/Tn1721 hybrid backbone. Within the hybrid backbone, a Tn6029-family transposon, identified here as Tn6029C abuts the sul1 gene in the 3'-Conserved Segment (-CS) of a class 1 integron generating a unique molecular signature that has only previously been observed in pASL01a, a small plasmid found in commensal E. coli in West Africa. From this common progenitor, independent IS26-mediated events created two novel transposons identified here as Tn6029D and Tn6222 in 2011C-3493 and 2009EL-2050 respectively. Analysis of RD1 within GI3 reveals IS26 has played a crucial role in the assembly of regions within the CRL.

Ecosystem responses to long-term nutrient management in an urban estuary: Tampa Bay, Florida, USA

NASA Astrophysics Data System (ADS)

Greening, H.; Janicki, A.; Sherwood, E. T.; Pribble, R.; Johansson, J. O. R.

2014-12-01

In subtropical Tampa Bay, Florida, USA, we evaluated restoration trajectories before and after nutrient management strategies were implemented using long-term trends in nutrient loading, water quality, primary production, and seagrass extent. Following citizen demands for action, reduction in wastewater nutrient loading of approximately 90% in the late 1970s lowered external total nitrogen (TN) loading by more than 50% within three years. Continuing nutrient management actions from public and private sectors were associated with a steadily declining TN load rate and with concomitant reduction in chlorophyll-a concentrations and ambient nutrient concentrations since the mid-1980s, despite an increase of more than 1 M people living within the Tampa Bay metropolitan area. Water quality (chlorophyll-a concentration, water clarity as indicated by Secchi disk depth, total nitrogen concentration and dissolved oxygen) and seagrass coverage are approaching conditions observed in the 1950s, before the large increases in human population in the watershed. Following recovery from an extreme weather event in 1997-1998, water clarity increased significantly and seagrass is expanding at a rate significantly different than before the event, suggesting a feedback mechanism as observed in other systems. Key elements supporting the nutrient management strategy and concomitant ecosystem recovery in Tampa Bay include: 1) active community involvement, including agreement about quantifiable restoration goals; 2) regulatory and voluntary reduction in nutrient loadings from point, atmospheric, and nonpoint sources; 3) long-term water quality and seagrass extent monitoring; and 4) a commitment from public and private sectors to work together to attain restoration goals. A shift from a turbid, phytoplankton-based system to a clear water, seagrass-based system that began in the 1980s following comprehensive nutrient loading reductions has resulted in a present-day Tampa Bay which looks and

Intramolecular transposition by a synthetic IS50 (Tn5) derivative

DOE Office of Scientific and Technical Information (OSTI.GOV)

Tomcsanyi, T.; Phadnis, S.H.; Berg, D.E.

1990-11-01

We report the formation of deletions and inversions by intramolecular transposition of Tn5-derived mobile elements. The synthetic transposons used contained the IS50 O and I end segments and the transposase gene, a contraselectable gene encoding sucrose sensitivity (sacB), antibiotic resistance genes, and a plasmid replication origin. Both deletions and inversions were associated with loss of a 300-bp segment that is designated the vector because it is outside of the transposon. Deletions were severalfold more frequent than inversions, perhaps reflecting constraints on DNA twisting or abortive transposition. Restriction and DNA sequence analyses showed that both types of rearrangements extended from onemore » transposon end to many different sites in target DNA. In the case of inversions, transposition generated 9-bp direct repeats of target sequences.« less

A simple and novel method for RNA-seq library preparation of single cell cDNA analysis by hyperactive Tn5 transposase.

PubMed

Brouilette, Scott; Kuersten, Scott; Mein, Charles; Bozek, Monika; Terry, Anna; Dias, Kerith-Rae; Bhaw-Rosun, Leena; Shintani, Yasunori; Coppen, Steven; Ikebe, Chiho; Sawhney, Vinit; Campbell, Niall; Kaneko, Masahiro; Tano, Nobuko; Ishida, Hidekazu; Suzuki, Ken; Yashiro, Kenta

2012-10-01

Deep sequencing of single cell-derived cDNAs offers novel insights into oncogenesis and embryogenesis. However, traditional library preparation for RNA-seq analysis requires multiple steps with consequent sample loss and stochastic variation at each step significantly affecting output. Thus, a simpler and better protocol is desirable. The recently developed hyperactive Tn5-mediated library preparation, which brings high quality libraries, is likely one of the solutions. Here, we tested the applicability of hyperactive Tn5-mediated library preparation to deep sequencing of single cell cDNA, optimized the protocol, and compared it with the conventional method based on sonication. This new technique does not require any expensive or special equipment, which secures wider availability. A library was constructed from only 100 ng of cDNA, which enables the saving of precious specimens. Only a few steps of robust enzymatic reaction resulted in saved time, enabling more specimens to be prepared at once, and with a more reproducible size distribution among the different specimens. The obtained RNA-seq results were comparable to the conventional method. Thus, this Tn5-mediated preparation is applicable for anyone who aims to carry out deep sequencing for single cell cDNAs. Copyright © 2012 Wiley Periodicals, Inc.

Interdisciplinary dialogue for education, collaboration, and innovation: Intelligent Biology and Medicine in and beyond 2013

PubMed Central

2013-01-01

The 2013 International Conference on Intelligent Biology and Medicine (ICIBM 2013) was held on August 11-13, 2013 in Nashville, Tennessee, USA. The conference included six scientific sessions, two tutorial sessions, one workshop, two poster sessions, and four keynote presentations that covered cutting-edge research topics in bioinformatics, systems biology, computational medicine, and intelligent computing. Here, we present a summary of the conference and an editorial report of the supplements to BMC Genomics and BMC Systems Biology that include 19 research papers selected from ICIBM 2013. PMID:24564388

NASA Provides Coast-to-Coast Coverage of Aug. 21 Solar Eclipse (Clarksville, TN – Austin Peay)

NASA Image and Video Library

2017-08-21

On Monday, Aug. 21, NASA provided coast-to-coast coverage of the solar eclipse across America – featuring views of the phenomenon from unique vantage points, including from the ground, from aircraft, and from spacecraft including the ISS, during a live broadcast seen on NASA Television and the agency’s website. This is footage from Austin Peay State University, in Clarksville, TN.

Measurements comparison of oxygenated volatile organic compounds at a rural site during the 1995 SOS Nashville Intensive

NASA Astrophysics Data System (ADS)

Apel, E. C.; Calvert, J. G.; Riemer, D.; Pos, W.; Zika, R.; Kleindienst, T. E.; Lonneman, W. A.; Fung, K.; Fujita, E.; Shepson, P. B.; Starn, T. K.; Roberts, P. T.

1998-09-01

Simultaneous measurements of oxygenated volatile organic compounds (OVOCs) by four independent research groups at the Youth Incorporated (YI) site during the 1995 Southern Oxidants Study Nashville Intensive, between July 4 and 30, 1995, provided a unique opportunity to compare results from different techniques. 2,4-Dinitrophenylhydrazine (DNPH) coated C18 (C18AtmAA) and Si-Gel (Si-GelMTE) cartridges were compared with each other and with two sorbent-based preconcentration gas chromatographic (GC) techniques coupled with mass spectrometric (MS) detection (GC/MSUM and GC/MSPU) or flame ionization detection GC/FIDUM· The experiment consisted of both a laboratory (part A) and an ambient air comparison (part B). In part A of the study, standard mixtures of OVOCs were diluted in a flowing gas stream of humidified, purified air, both with and without addition of O3, and distributed to participant's instrumentation. Concentrations were compared with the expected values based on known cylinder concentrations and dilution factors. In part B of the study, the instruments sampled ambient air from a common glass manifold. Species intercompared were formaldehyde, acetaldehyde, acetone, and propanal. The C18AtmAA data were typically higher than the Si-GelMTE data for the four intercompared compounds, and possible explanations are given. Agreement between the cartridge systems and the GC/MS, GC/FID systems for comeasured compounds was poorer than expected but improved towards the end of the experiment. The GC/MS systems tracked each other well for acetone, but there were differences in the absolute concentration values. These results show that improvements in the accuracy and comparability of techniques should be of high priority.

Genomic and transcriptomic differences in community acquired methicillin resistant Staphylococcus aureus USA300 and USA400 strains.

PubMed

Jones, Marcus B; Montgomery, Christopher P; Boyle-Vavra, Susan; Shatzkes, Kenneth; Maybank, Rosslyn; Frank, Bryan C; Peterson, Scott N; Daum, Robert S

2014-12-19

Staphylococcus aureus is a human pathogen responsible for substantial morbidity and mortality through its ability to cause a number of human infections including bacteremia, pneumonia and soft tissue infections. Of great concern is the emergence and dissemination of methicillin-resistant Staphylococcus aureus strains (MRSA) that are resistant to nearly all β-lactams. The emergence of the USA300 MRSA genetic background among community associated S. aureus infections (CA-MRSA) in the USA was followed by the disappearance of USA400 CA-MRSA isolates. To gain a greater understanding of the potential fitness advantages and virulence capacity of S. aureus USA300 clones, we performed whole genome sequencing of 15 USA300 and 4 USA400 clinical isolates. A comparison of representative genomes of the USA300 and USA400 pulsotypes indicates a number of differences in mobile genome elements. We examined the in vitro gene expression profiles by microarray hybridization and the in vivo transcriptomes during lung infection in mice of a USA300 and a USA400 MRSA strain by performing complete genome qRT-PCR analysis. The unique presence and increased expression of 6 exotoxins in USA300 (12- to 600-fold) compared to USA400 may contribute to the increased virulence of USA300 clones. Importantly, we also observed the up-regulation of prophage genes in USA300 (compared with USA400) during mouse lung infection (including genes encoded by both prophages ΦSa2usa and ΦSa3usa), suggesting that these prophages may play an important role in vivo by contributing to the elevated virulence characteristic of the USA300 clone. We observed differences in the genetic content of USA300 and USA400 strains, as well as significant differences of in vitro and in vivo gene expression of mobile elements in a lung pneumonia model. This is the first study to document the global transcription differences between USA300 and USA400 strains during both in vitro and in vivo growth.

The Teratology Society 2007 strategic planning session: a desire to inspire.

PubMed

2008-05-01

On April 18-20, 2007, the Teratology Society held its third strategic planning session (SPS) in San Diego, CA. The purpose of this session was to build on the successful work generated by the previous strategic plans [Nashville, TN 2002 and Cincinnati, OH 1997] and importantly, to provide a path forward to inspire the Society, create deeper connections with members that speak to their individual passion for the science of teratology and to increase the Society's visibility within the larger scientific community. The following summary report provides an overview of the session's pre-work, objective, and discussions. A total of 24 attendees were present at the session. The group included representation from Council, various committees and different members constituencies. This plan and the activities subsequent to the session will provide a path forward for our Society for the next five years.

High-Level Heat Resistance of Spores of Bacillus amyloliquefaciens and Bacillus licheniformis Results from the Presence of a spoVA Operon in a Tn1546 Transposon

PubMed Central

Berendsen, Erwin M.; Koning, Rosella A.; Boekhorst, Jos; de Jong, Anne; Kuipers, Oscar P.; Wells-Bennik, Marjon H. J.

2016-01-01

Bacterial endospore formers can produce spores that are resistant to many food processing conditions, including heat. Some spores may survive heating processes aimed at production of commercially sterile foods. Recently, it was shown that a spoVA operon, designated spoVA2mob, present on a Tn1546 transposon in Bacillus subtilis, leads to profoundly increased wet heat resistance of B. subtilis spores. Such Tn1546 transposon elements including the spoVA2mob operon were also found in several strains of Bacillus amyloliquefaciens and Bacillus licheniformis, and these strains were shown to produce spores with significantly higher resistances to wet heat than their counterparts lacking this transposon. In this study, the locations and compositions of Tn1546 transposons encompassing the spoVA2mob operons in B. amyloliquefaciens and B. licheniformis were analyzed. Introduction of these spoVA2mob operons into B. subtilis 168 (producing spores that are not highly heat resistant) rendered mutant 168 strains that produced high-level heat resistant spores, demonstrating that these elements in B. amyloliquefaciens and B. licheniformis are responsible for high level heat resistance of spores. Assessment of growth of the nine strains of each species between 5.2°C and 57.7°C showed some differences between strains, especially at lower temperatures, but all strains were able to grow at 57.7°C. Strains of B. amyloliquefaciens and B. licheniformis that contain the Tn1546 elements (and produce high-level heat resistant spores) grew at temperatures similar to those of their Tn1546-negative counterparts that produce low-level heat resistant spores. The findings presented in this study allow for detection of B. amyloliquefaciens and B. licheniformis strains that produce highly heat resistant spores in the food chain. PMID:27994575

A Phase I Study of Unimolecular Pentavalent (Globo-H-GM2-sTn-TF-Tn) Immunization of Patients with Epithelial Ovarian, Fallopian Tube, or Peritoneal Cancer in First Remission.

PubMed

O'Cearbhaill, Roisin E; Ragupathi, Govind; Zhu, Jianglong; Wan, Qian; Mironov, Svetlana; Yang, Guangbin; Spassova, Maria K; Iasonos, Alexia; Kravetz, Sara; Ouerfelli, Ouathek; Spriggs, David R; Danishefsky, Samuel J; Sabbatini, Paul J

2016-04-22

We conducted a phase I study in ovarian cancer patients to evaluate the safety and immunogenicity of a synthetic unimolecular pentavalent carbohydrate vaccine (Globo-H, GM2, sTn, TF, and Tn) supported on a peptide backbone, conjugated to keyhole limpet haemocyanin (KLH), and mixed with immunological adjuvant QS-21. Twenty-four advanced-stage, poor-risk, first-remission ovarian cancer patients were enrolled from January 2011-Septermber 2013. Three dose levels were planned (25, 50, 100 mcg) with three cohorts of six patients each, with an additional 6-patient expansion cohort at the MTD. ELISA serologic IgM and IgG responses for each antigen was defined as positive response if antibody titers were ≥1:80 over the respective patient's pre-vaccination serum. The study would be considered positive if at least four of 12 patients treated at the MTD showed immune responses for at least three of the five antigens. Twenty-four patients (median age, 54 years [range, 36-68]) were included in the safety analysis. Histology was high-grade serous in 22 patients (92%); 18 had stage III and six stage IV disease. The vaccine was well-tolerated at all doses, with no DLTs. At the highest treated dose, IgG and/or IgM responses were recorded against ≥3 antigens in 9/12 patients (75%), ≥4 in 7/12 (58%), and 5 in 3/12 (25%). With a median follow-up of 19 months (range, 2-39), 20 patients (83%) recurred and six (25%) died. The unimolecular pentavalent vaccine construct was shown to be safe and immunogenic. Such a construct greatly simplifies regulatory requirements and manufacturing, facilitates scalability, and provides adaptability.

Use of the multipurpose transposon Tn KPK2 for the mutational analysis of chromosomal regions upstream and downstream of the sipF gene in Bradyrhizobium japonicum.

PubMed

Müller, P

2004-04-01

The DNA regions upstream and downstream of the Bradyrhizobium japonicum gene sipF were cloned by in vivo techniques and subsequently sequenced. In order to study the function of the predicted genes, a new transposon for in vitro mutagenesis, Tn KPK2, was constructed. This mutagenesis system has a number of advantages over other transposons. Tn KPK2 itself has no transposase gene, making transposition events stable. Extremely short inverted repeats minimize the length of the transposable element and facilitate the determination of the nucleotide sequence of the flanking regions. Since the transposable element carries a promoterless ' phoA reporter gene, the appearance of functional PhoA fusion proteins indicates that Tn KPK2 has inserted in a gene encoding a periplasmic or secreted protein. Although such events are extremely rare, because the transposon has to insert in-frame, in the correct orientation, and at an appropriate location in the target molecule, a direct screening procedure on agar indicator plates permits the identification of candidate clones from large numbers of colonies. In this study, Tn KPK2 was used for the construction of various symbiotic mutants of B. japonicum. One of the mutant strains, A2-10, which is defective in a gene encoding a protein that comigrates with bacterioferritin ( bcpB), was found to induce the formation of small and ineffective nodules.

[Establishment of Reference Value of Hs-cTnT in Sichuan Region and Its Diagnostic Value in Patients with Chest Pain].

PubMed

Guo, Ying; Du, Xiang-Yang; Huang, Hua-Lan; Wang, Wei-Qing; Nie, Xin; Li, Gui-Xing

2017-11-01

To establish the reference value of high sensitive cardiac troponin T (hs-cTnT) and the efficiency of reference value in the diagnosis of chest pain. Volunteers from eight independent communities in Chengdu,Sichuan were selected with detailed records of physical examination,electrocardiogram,ultrasound examination. The level of hs-cTnT for healthy volunteers was tested to determine ninety-ninth percentile references according to sex and ages. 2 249 patients with chest pain in the emergency department of Western China Hospital from July 2009 to July 2014 were enrolled to measure the efficiency of reference value for diagnosing acute myocardial infarction (AMI). There were 1 305 volunteers included finally. Among them,the mean hs-cTnT level of male was 4.3 (3.2-5.9) ng/L,which was significantly higher than that of female 3.0 (3.0-3.1) ng/L ( P <0.01) . The correlation coefficient between age and hs-cTnT level was 0.43 (male) and 0.29 (female),and the P -value was less than 0.01. The 99th percentile values of male were 10.8 ng/L,15.4 ng/L and 19.7 ng/L for <45 yr.,45-<60 yr. and ≥60 yr.,respectively. Those values of female were 4.6 ng/L,8.9 ng/L,18.8 ng/L,respectively. There was no difference in sensitivity and specificity between the value we figured out and manufactures provided (14.0 ng/L) for those<60 yr.. For the patients ≥60 yr.,the sensitivity and negative predictive value did not show diversity ( P >0.05) but the specificity and positive predictive value showed significant difference (male: 0.67 vs. 0.56 and 0.83 vs. 0.79, P <0.05;female:0.75 vs. 0.68 and 0.74 vs. 0.69, P <0.05). We recommends that the ninety-ninth percentile reference value of patients<60 yr. should be 14.0 ng/L,while 20.0 ng/L for those patients≥60 yr.

Green digital signage using nanoparticle embedded narrow-gap field sequential TN-LCDs

NASA Astrophysics Data System (ADS)

Kobayashi, Shunsuke; Shiraishi, Yukihide; Sawai, Hiroya; Toshima, Naoki; Okita, Masaya; Takeuchi, Kiyofumi; Takatsu, Haruyoshi

2012-03-01

We have fabricated field sequential color (FSC)-LCDs using cells and modules of narrow-gap TN-LCDs with and without doping the nanoparticles of PCyD-ZrO2 and AF-SiO2. It is shown that the FSC-LCD exhibits a high optical efficiency of OE=4.5 that is defined as OE=[Luminance]/[W/m2]=(cd/W). This figure may provide us a good reference or to clear the Energy Star Program Version 5-3 that issues a guideline: LCD with 50 inch on the diagonal consumes the energy of 108W. Through this research it is claimed that our FSC=LCD may be a novel green digital signage.

'Test n Treat (TnT)': a cluster-randomised feasibility trial of frequent, rapid-testing and same-day, on-site treatment to reduce rates of chlamydia in high-risk further education college students: statistical analysis plan.

PubMed

Phillips, Rachel; Oakeshott, Pippa; Kerry-Barnard, Sarah; Reid, Fiona

2018-06-05

There are high rates of sexually transmitted infections (STIs) in ethnically diverse, sexually active students aged 16-24 years attending London further education (FE) colleges. However, uptake of chlamydia screening remains low. The TnT study aims to assess the feasibility of conducting a future trial in FE colleges to investigate if frequent, rapid, on-site testing and treatment (TnT) reduces chlamydia rates. This article presents the statistical analysis plan for the main study publication as approved and signed off by the Trial Management Group prior to the first data extraction for the final report. TnT is a cluster-randomised feasibility trial conducted over 7 months with parallel qualitative and economic assessments. Colleges will be randomly allocated into the intervention (TnT) or the control group (no TnT). Six FE colleges in London will be included. At each college for 2 days, 80 consecutive sexually active students aged 16-24 years (total 480 students across all six colleges) will be recruited from public areas and asked to provide baseline samples. One and 4 months after recruitment intervention colleges will be visited on two consecutive days by the TnT team where participating students will be texted and invited to come for same-day, on-site, rapid chlamydia testing and, if positive, treatment. Participants in the control colleges will receive 'thank you' texts 1 and 4 months after recruitment. Seven months after recruitment, participants from both groups will be invited to complete questionnaires and provide samples for TnT. All samples will be tested, and same-day treatment offered to participants with positive results. Key feasibility outcomes include: recruitment rates, testing and treatment uptake rates (at 1 and 4 months) and follow-up rates (at 7 months). ISRCTN 58038795 . Registered on 31 August 2016.

How Does the Fast Track Intervention Prevent Adverse Outcomes in Young Adulthood?

PubMed Central

Sorensen, Lucy C.; Dodge, Kenneth A.

2015-01-01

Numerous studies have shown that childhood interventions can foster improved outcomes in adulthood. Less well understood is precisely how – that is, through which developmental pathways – these interventions work. This study assesses mechanisms by which the Fast Track project (n=891), a randomized intervention in the early 1990s for high-risk children in four communities (Durham, NC; Nashville, TN; rural PA; and Seattle, WA), reduced delinquency, arrests, and health and mental health service utilization in adolescence through young adulthood (ages 12–20). A decomposition of treatment effects indicates that about a third of Fast Track’s impact on later crime outcomes can be accounted for by improvements in social and self-regulation skills during childhood (ages 6–11), such as prosocial behavior, emotion regulation and problem solving. These skills proved less valuable for the prevention of mental and physical health problems. PMID:26670938

Advances in systems biology: computational algorithms and applications.

PubMed

Huang, Yufei; Zhao, Zhongming; Xu, Hua; Shyr, Yu; Zhang, Bing

2012-01-01

The 2012 International Conference on Intelligent Biology and Medicine (ICIBM 2012) was held on April 22-24, 2012 in Nashville, Tennessee, USA. The conference featured six technical sessions, one tutorial session, one workshop, and 3 keynote presentations that covered state-of-the-art research activities in genomics, systems biology, and intelligent computing. In addition to a major emphasis on the next generation sequencing (NGS)-driven informatics, ICIBM 2012 aligned significant interests in systems biology and its applications in medicine. We highlight in this editorial the selected papers from the meeting that address the developments of novel algorithms and applications in systems biology.

EnviroAtlas -- Memphis, TN (2012) -- One Meter Resolution Urban Land Cover Data Web Service

EPA Pesticide Factsheets

This EnviroAtlas web service supports research and online mapping activities related to EnviroAtlas (https://www.epa.gov/enviroatlas ). The Memphis, TN EnviroAtlas One Meter-scale Urban Land Cover (MULC) dataset comprises 2,733 km2 around the city of Memphis, surrounding towns, and rural areas. These leaf-on LC data and maps were derived from 1-m pixel, four-band (red, green, blue, and near-infrared) aerial photography acquired from the United States Department of Agriculture (USDA) National Agriculture Imagery Program (NAIP) on four dates in 2012: June 15, June 18, June 21 and June 23, and one date in 2013: July 12. Three separate LiDAR (Light Detection and Ranging) data sets collected on February 19, 2009 00e2?? August 2, 2010, December 1-2, 2011 and January 23-24, 2012 were integrated for Shelby Co., TN, Crittenden Co., AR, and DeSoto Co, MS. Five MULC classes were mapped directly from the NAIP and LiDAR data: Water, Impervious, Soil, Trees, and Grass/Herbaceous. Agriculture was derived from USDA Common Land Unit (CLU) data. Woody and emergent wetlands were copied from existing National Wetlands Inventory (NWI) data. Analysis of a random sampling of 612 photo-interpreted land cover reference points yielded an overall users accuracy of 86.9%. This dataset was produced by the US EPA to support research and online mapping activities related to EnviroAtlas. EnviroAtlas (https://www.epa.gov/enviroatlas) allows the user to interact with a web-based, easy-to-u

Oxidized zirconium on ceramic; Catastrophic coupling.

PubMed

Ozden, V E; Saglam, N; Dikmen, G; Tozun, I R

2017-02-01

Oxidized zirconium (Oxinium™; Smith & Nephew, Memphis, TN, USA) articulated with polyethylene in total hip arthroplasty (THA) appeared to have the potential to reduce wear dramatically. The thermally oxidized metal zirconium surface is transformed into ceramic-like hard surface that is resistant to abrasion. The exposure of soft zirconium metal under hard coverage surface after the damage of oxidized zirconium femoral head has been described. It occurred following joint dislocation or in situ succeeding disengagement of polyethylene liner. We reported three cases of misuse of Oxinium™ (Smith & Nephew, Memphis, TN, USA) heads. These three cases resulted in catastrophic in situ wear and inevitable failure although there was no advice, indication or recommendation for this use from the manufacturer. Copyright © 2016 Elsevier Masson SAS. All rights reserved.

Characterization of Tn3000, a Transposon Responsible for blaNDM-1 Dissemination among Enterobacteriaceae in Brazil, Nepal, Morocco, and India.

PubMed

Campos, Juliana Coutinho; da Silva, Maria José Félix; dos Santos, Paulo Roberto Nascimento; Barros, Elaine Menezes; Pereira, Mayne de Oliveira; Seco, Bruna Mara Silva; Magagnin, Cibele Massotti; Leiroz, Leonardo Kalab; de Oliveira, Théo Gremen Mimary; de Faria-Júnior, Célio; Cerdeira, Louise Teixeira; Barth, Afonso Luís; Sampaio, Suely Carlos Ferreira; Zavascki, Alexandre Prehn; Poirel, Laurent; Sampaio, Jorge Luiz Mello

2015-12-01

In Enterobacteriaceae, the blaNDM genes have been found in many different genetic contexts, and a wide diversity of plasmid scaffolds bearing those genes has been found. In August 2013, we identified NDM-1-producing Escherichia coli and Enterobacter hormaechei strains from a single rectal swab sample from a patient hospitalized in Rio de Janeiro, Brazil, who had no history of travel abroad. Complete DNA sequencing using the Illumina platform and annotation of the two plasmids harboring the blaNDM-1 gene, one from each strain, showed that they belonged to incompatibility groups IncFIIK and IncX3 and harbored a novel transposon named Tn3000. Similar genetic structures have been identified among other isolates in Brazil but also on plasmids from other continents. Our findings suggest that the blaNDM-1 gene may be transmitted by Tn3000 in different parts of the world. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

77 FR 6587 - Startek USA, Inc. Alexandria, LA; Startek USA, Inc., Collinsville, VA; Amended Certification...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-02-08

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-75,089; TA-W-75,089A] Startek USA, Inc. Alexandria, LA; Startek USA, Inc., Collinsville, VA; Amended Certification Regarding Eligibility... for Worker Adjustment Assistance on January 26, 2011, applicable to workers of StarTek USA, Inc...

The impact of systems-of-care on pharmacoinvasive management with streptokinase: The subgroup analysis of the TN-STEMI programme.

PubMed

Raja, Deep Chandh; Subban, Vijayakumar; Victor, Suma M; Joseph, George; Thomson, Viji Samuel; Kannan, Kumaresan; Gnanaraj, Justin Paul; Veerasekar, Ganesh; Thenpally, Jose G; Livingston, Nandhini; Nallamothu, Brahmajee K; Alexander, Thomas; Mullasari, Ajit S

We evaluated the impact of implementation of the TN-STEMI programme on various characteristics of the pharmacoinvasive group by comparing clinical as well as angiographic outcomes between the pre- and post-implementation groups. The TN-STEMI programme involved 2420 patients of which 423 patients had undergone a pharmacoinvasive strategy of reperfusion. Of these, 407 patients had a comprehensive blinded core-lab evaluation of their angiograms post-lysis and clinical evaluation of various parameters including time-delays and adverse cardio- and cerebro-vascular events at 1year. Streptokinase was used as the thrombolytic agent in 94.6% of the patients. In the post-implementation phase, there was a significant improvement in 'First medical contact (FMC)-to-ECG' (11 vs. 5min, p<0.001) and 'Lysis-to-angiogram' (98.3 vs. 18.2h, p<0.001) times. There was also a significant improvement in the number of coronary angiograms performed within 24h (20.7% vs. 69.3%, p<0.001). The 'Time-to-FMC' (160 vs. 135min, p=0.07) and 'Total ischemic time' (210 vs. 176min, p=0.22) also showed a decreasing trend. IRA patency rate (70.2% vs. 86%, p<0.001) and thrombus burden (TIMI grade 0: 49.1% vs. 73.4%, p<0.001) were superior in this group. The MACCE rates were similar except for fewer readmissions (29.8% vs. 12.6%, p=0.0002) and target revascularizations at 1year (4.8% vs. none, p=0.002) in the post-implementation group. The implementation of a system-of-care (hub-and-spoke model) in the pharmacoinvasive group of the TN-STEMI programme demonstrated shorter lysis-to-angiogram times, better TIMI flow patterns and lower thrombus burden in the post-implementation phase. Copyright © 2017. Published by Elsevier B.V.

A large inversion in the linear chromosome of Streptomyces griseus caused by replicative transposition of a new Tn3 family transposon.

PubMed

Murata, M; Uchida, T; Yang, Y; Lezhava, A; Kinashi, H

2011-04-01

We have comprehensively analyzed the linear chromosomes of Streptomyces griseus mutants constructed and kept in our laboratory. During this study, macrorestriction analysis of AseI and DraI fragments of mutant 402-2 suggested a large chromosomal inversion. The junctions of chromosomal inversion were cloned and sequenced and compared with the corresponding target sequences in the parent strain 2247. Consequently, a transposon-involved mechanism was revealed. Namely, a transposon originally located at the left target site was replicatively transposed to the right target site in an inverted direction, which generated a second copy and at the same time caused a 2.5-Mb chromosomal inversion. The involved transposon named TnSGR was grouped into a new subfamily of the resolvase-encoding Tn3 family transposons based on its gene organization. At the end, terminal diversity of S. griseus chromosomes is discussed by comparing the sequences of strains 2247 and IFO13350.

Tropospheric Ozone Pollution Transport Traced from the TOMS (Total Ozone Mapping Spectrometer) Instrument During the Nashville-1999 Campaign

NASA Technical Reports Server (NTRS)

Thompson, Anne M.; Frolov, A. D.; Hudson, R. D.; Witte, J. C.; Einaudi, Franco (Technical Monitor)

2000-01-01

Over the past several years, we have developed two new tropospheric ozone retrievals from the TOMS (Total Ozone Mapping Spectrometer) satellite instrument that are of sufficient resolution to follow pollution episodes. The modified-residual technique [Hudson and Thompson, 1998; Thompson and Hudson, 1999] uses v. 7 TOMS total ozone and is applicable to tropical regimes in which the wave-one pattern in total ozone is observed. The TOMS-direct method [("TDOT" = TOMS Direct Ozone in the Troposphere; Frolov et al., 2000] represents a new algorithm that uses TOMS radiances directly (i.e., not previously processed for TOMS ozone) to extract tropospheric ozone in regions of constant stratospheric ozone and tropospheric ozone displaying high mixing ratios and variability characteristic of pollution. These events tend to occur in certain meteorological regimes. For example, mid-latitude pollution usually occurs on the backside of subtropical fronts, as low pv, usually moist air intrudes to the extra-tropics. July 1999 was a month characterized by robust pollution in the eastern US, with high ozone, as detected by TOMS, originating over south central states and moving up the Atlantic seaboard. This corresponds to 50-80 DU in tropospheric ozone column depth. In most cases, further transport occurred to the North Atlantic, with ozone plumes traveling to western Europe in 4-5 days. Examples of high ozone and transit across boundaries within the US, as well as US->Europe, give a regional context for model results and field measurements taken in the SE US during the Nashville-1999 campaign period. Validation of the TDOT maps is made with ozonesondes taken during that time. TDOT maps also show ozone pollution from Asia traveling to the western US in July 1999.

MRSA USA300 at Alaska Native Medical Center, Anchorage, Alaska, USA, 2000–2006

PubMed Central

Rudolph, Karen M.; Hennessy, Thomas W.; Zychowski, Diana L.; Asthi, Karthik; Boyle-Vavra, Susan; Daum, Robert S.

2012-01-01

To determine whether methicillin-resistant Staphylococcus aureus (MRSA) USA300 commonly caused infections among Alaska Natives, we examined clinical MRSA isolates from the Alaska Native Medical Center, Anchorage, during 2000–2006. Among Anchorage-region residents, USA300 was a minor constituent among MRSA isolates in 2000–2003 (11/68, 16%); by 2006, USA300 was the exclusive genotype identified (10/10). PMID:22264651

Radiation Measurements in Simulated Ablation Layers

DTIC Science & Technology

2010-12-06

J.Spacecraft & Rockets, V35, No 6, 1998, pp 729-735. D‟Souza MG, Eichmann TN, Mudford NR, Potter DF, Morgan RG, McIntyre TJ, Jacobs PA (2009...gases. D. Phil Thesis. Oxford University 1976 Potter, D., Eichmann , T., Brandis, A., Morgan, R., Jacobs, P., McIntyre, T., “Simulation of radiating...Heatshield Material. 46th AIAA Aerospace Sciences Meeting and Exhibit, AIAA2008-1202, Reno, USA. D‟Souza, M.G., Eichmann , T.N., Mudford, N.R., Potter

Plinian vs. phreatomagmatic eruptions at Grímsvötn volcano, Iceland

NASA Astrophysics Data System (ADS)

Haddadi, Baptiste; Sigmarsson, Olgeir; Larsen, Guðrún

2016-04-01

Grímsvötn is a subglacial central volcano located under the Vatnajökull ice cap, above the assumed centre of the Iceland mantle plume. Historical explosive eruptions are mostly of phreatomagmatic character whereas pure magmatic behaviour may characterize the largest eruptions. What causes this different eruption behaviour is uncertain. Here, we report petrological estimates of crystallization depth and volatile degassing as recorded by sulfur concentrations in melt inclusions (MI) hosted by ferromagnesian minerals and the groundmass glass. Tephra from four eruptions, AD 1823, 1873, 2004 and 2011, were selected. The 2011 and 1873 are the largest known historical eruptions, whereas the 2004 eruption is probably amongst the smallest. The repose time preceding those eruptions is surprisingly similar, or 6 to 7 years, and the major-element compositions are uniform. Plagioclase, clinopyroxene (cpx) and olivine are the three coexisting phases at the liquidus in the quartz-tholeiites of Grímsvötn. The cpx-melt geothermobarometer (Putirka 2008) applied to the 2011 tephra reveals that cpx crystallized over a large range of P from 60 to 640 MPa (depth range: 1.7-18km) and T between 1060 and 1175°C before the Plinian eruption, therefore mobilizing the entire crustal magma system. In contrast, the phreatomagmatic tephra do not record the shallowest crystallization but interestingly all four tephra have identical median entrapment pressure of approximately 400 MPa. Therefore, the depth from which the magma bodies are derived, does not explain the difference in explosivity between those eruptions nor the variable magma volume (V) produced. Sulfur concentrations in MI are only slightly higher in the Plinian products, the difference (10%) being insufficient to explain the different eruption regimes. The ΔS, the difference between the maximum S concentrations in MI and the mean of the groundmass glass for a given eruption, is higher in the Plinian tephra. Based on literature

Bio-preservative effect of the essential oil of the endemic Mentha piperita used alone and in combination with BacTN635 in stored minced beef meat.

PubMed

Smaoui, Slim; Hsouna, Anis Ben; Lahmar, Aida; Ennouri, Karim; Mtibaa-Chakchouk, Ahlem; Sellem, Imen; Najah, Soumaya; Bouaziz, Mohamed; Mellouli, Lotfi

2016-07-01

The major compounds in Mentha piperita essential oil (EOMP) were menthol (33.59%) and iso-menthone (33%). The biopreservative effect of EOMP used alone at 0.25 or 0.5% and in combination with the semi-purified bacteriocin BacTN635 at 500 or 1000AU/g, on minced beef meat was evaluated by microbiological, physicochemical and sensory analyses during storage at 4°C for 21days. EOMP used alone limited the microbial deterioration of minced meat (P<0.05). Furthermore, the combination between EOMP and BacTN635 led to a decrease in TBARS values and slowed down the accumulation of MetMb. This combination was more efficient (P<0.05) against microflora proliferation and enhanced the sensory acceptability extending thus the shelf life of meat beef by approximately 7days. On the basis of these results, physicochemical and sensorial parameters could be used for constructing regression models to predict overall acceptability. Overall, the strongest preservative effect was achieved by using the combination of EOMP at 0.5% with BacTN535 at 1000AU/g. Copyright © 2016 Elsevier Ltd. All rights reserved.

Evaluation of Stream Loads Used to Calibrate a SPARROW Model for California, USA

NASA Astrophysics Data System (ADS)

Domagalski, J. L.; Saleh, D.

2012-12-01

A SPARROW (Spatially Referenced Regression on Watershed Attributes) Model is being developed for California. The model will be used to understand how Total Nitrogen (TN) and Total Phosphorus (TP) are transported from land to water from sources such as the atmosphere, fertilizer, soils, wastewater treatment facilities, etc., and relies on accurate calibration of mass loads obtained from water sampling at gauging stations in order to link mass at a location to upstream sources. Prior to input to the SPARROW model, the mass loads are calculated separately using a five-parameter log linear multi-regression model utilizing discharge, chemical measurements, time, and seasonal adjustments to obtain the best fit for the relationship of discharge and concentration. The gauging stations are situated in three ecological management zones as defined by the U.S. Environmental Protection Agency: the Western Forested Mountains, the Central Valley, and the Xeric West. Load models for nitrogen have at times been shown to be positively biased when the form of TN is predominately nitrate. The regions under study have different sources of nitrogen, which will affect the form of TN transported. Some stream segments are natural settings (forested), while others are highly influenced by agriculture and urban (Central Valley) settings and others by arid climate (Xeric). These differences affect the form of TN transported (dissolved as nitrate or suspended in the form of organic nitrogen), and hence it is expected that the efficiency of the discharge-load model may not be uniform at all locations. Less than 10% of the TN is in the form of nitrate in streams of the western forested mountains, but about 30% is nitrate in the Central Valley and about 40% in the arid region. Model efficiency was evaluated using the Nash Sutcliffe (NS) equation, which examines the square of the residuals of modeled results and observed values after transforming the logarithm of loads back to the actual data

Utility of Cardiac Troponin to Predict Drug Overdose Mortality

PubMed Central

Stimmel, Barry; Hoffman, Robert S.; Vlahov, David

2016-01-01

Drug overdose is now the leading cause of injury-related mortality in the USA, but the prognostic utility of cardiac biomarkers is unknown. We investigated whether serum cardiac troponin I (cTnI) was associated with overdose mortality. This prospective observational cohort studied adults with suspected acute drug overdose at two university hospital emergency departments (ED) over 3 years. The endpoint was in-hospital mortality, which was used to determine test characteristics of initial/peak cTnI. There were 437 overdoses analyzed, of whom there were 20 (4.6 %) deaths. Mean initial cTnI was significantly associated with mortality (1.2 vs. 0.06 ng/mL, p <0.001), and the ROC curve revealed excellent cTnI prediction of mortality (AUC 0.87, CI 0.76–0.98). Test characteristics for initial cTnI (90 % specificity, 99 % negative predictive value) were better than peak cTnI (88.2 % specificity, 99.2 % negative predictive value), and initial cTnI was normal in only one death out of the entire cohort (1/437, CI 0.1–1.4 %). Initial cTnI results were highly associated with drug overdose mortality. Future research should focus on high-risk overdose features to optimize strategies for utilization of cTnI as part of the routine ED evaluation for acute drug overdose. PMID:26541348

Can a Point-of-Care Troponin I Assay be as Good as a Central Laboratory Assay? A MIDAS Investigation

PubMed Central

Diercks, Deborah; Birkhahn, Robert; Singer, Adam J.; Hollander, Judd E.; Nowak, Richard; Safdar, Basmah; Miller, Chadwick D.; Peberdy, Mary; Counselman, Francis; Chandra, Abhinav; Kosowsky, Joshua; Neuenschwander, James; Schrock, Jon; Lee-Lewandrowski, Elizabeth; Arnold, William; Nagurney, John

2016-01-01

Background We aimed to compare the diagnostic accuracy of the Alere Triage Cardio3 Tropinin I (TnI) assay (Alere, Inc., USA) and the PathFast cTnI-II (Mitsubishi Chemical Medience Corporation, Japan) against the central laboratory assay Singulex Erenna TnI assay (Singulex, USA). Methods Using the Markers in the Diagnosis of Acute Coronary Syndromes (MIDAS) study population, we evaluated the ability of three different assays to identify patients with acute myocardial infarction (AMI). The MIDAS dataset, described elsewhere, is a prospective multicenter dataset of emergency department (ED) patients with suspected acute coronary syndrome (ACS) and a planned objective myocardial perfusion evaluation. Myocardial infarction (MI) was diagnosed by central adjudication. Results The C-statistic with 95% confidence intervals (CI) for diagnosing MI by using a common population (n=241) was 0.95 (0.91-0.99), 0.95 (0.91-0.99), and 0.93 (0.89-0.97) for the Triage, Singulex, and PathFast assays, respectively. Of samples with detectable troponin, the absolute values had high Pearson (RP) and Spearman (RS) correlations and were RP =0.94 and RS=0.94 for Triage vs Singulex, RP =0.93 and RS=0.85 for Triage vs PathFast, and RP =0.89 and RS=0.73 for PathFast vs Singulex. Conclusions In a single comparative population of ED patients with suspected ACS, the Triage Cardio3 TnI, PathFast, and Singulex TnI assays provided similar diagnostic performance for MI. PMID:27374704

Can a Point-of-Care Troponin I Assay be as Good as a Central Laboratory Assay? A MIDAS Investigation.

PubMed

Peacock, W Frank; Diercks, Deborah; Birkhahn, Robert; Singer, Adam J; Hollander, Judd E; Nowak, Richard; Safdar, Basmah; Miller, Chadwick D; Peberdy, Mary; Counselman, Francis; Chandra, Abhinav; Kosowsky, Joshua; Neuenschwander, James; Schrock, Jon; Lee-Lewandrowski, Elizabeth; Arnold, William; Nagurney, John

2016-09-01

We aimed to compare the diagnostic accuracy of the Alere Triage Cardio3 Tropinin I (TnI) assay (Alere, Inc., USA) and the PathFast cTnI-II (Mitsubishi Chemical Medience Corporation, Japan) against the central laboratory assay Singulex Erenna TnI assay (Singulex, USA). Using the Markers in the Diagnosis of Acute Coronary Syndromes (MIDAS) study population, we evaluated the ability of three different assays to identify patients with acute myocardial infarction (AMI). The MIDAS dataset, described elsewhere, is a prospective multicenter dataset of emergency department (ED) patients with suspected acute coronary syndrome (ACS) and a planned objective myocardial perfusion evaluation. Myocardial infarction (MI) was diagnosed by central adjudication. The C-statistic with 95% confidence intervals (CI) for diagnosing MI by using a common population (n=241) was 0.95 (0.91-0.99), 0.95 (0.91-0.99), and 0.93 (0.89-0.97) for the Triage, Singulex, and PathFast assays, respectively. Of samples with detectable troponin, the absolute values had high Pearson (R(P)) and Spearman (R(S)) correlations and were R(P)=0.94 and R(S)=0.94 for Triage vs Singulex, R(P)=0.93 and R(S)=0.85 for Triage vs PathFast, and R(P)=0.89 and R(S)=0.73 for PathFast vs Singulex. In a single comparative population of ED patients with suspected ACS, the Triage Cardio3 TnI, PathFast, and Singulex TnI assays provided similar diagnostic performance for MI.

Temporal Trends in Age at HIV Diagnosis in Cohorts in the United States, the Caribbean, and Central and South America.

PubMed

Crabtree-Ramírez, Brenda; Vega, Yanink Neried Caro; Shepherd, Bryan E; Turner, Megan; Carriquiry, Gabriela; Fink, Valeria; Luz, Paula M; Cortes, Claudia P; Rouzier, Vanessa; Padgett, Denis; Jayathilake, Karu; McGowan, Catherine C; Person, Anna K

2015-09-01

In the United States (USA), the age of those newly diagnosed with HIV is changing, particularly among men who have sex with men (MSM). A retrospective analysis included HIV-infected adults from seven sites in the Caribbean, Central and South America network (CCASAnet) and the Vanderbilt Comprehensive Care Clinic (VCCC-Nashville, Tennessee, USA). We estimated the proportion of patients <25 years at HIV diagnosis by calendar year among the general population and MSM. 19,466 (CCASAnet) and 3,746 (VCCC) patients were included. The proportion <25 years at diagnosis in VCCC increased over time for both the general population and MSM (p < 0.001). Only in the Chilean site for the general population and the Brazilian site for MSM were similar trends seen. Subjects <25 years of age at diagnosis were less likely to be immunocompromised at enrollment at both the VCCC and CCASAnet. Recent trends in the USA of greater numbers of newly diagnosed young patients were not consistently observed in Latin America and the Caribbean. Prevention efforts tailored to young adults should be increased.

The Boston Harbor Project, and large decreases in loadings of eutrophication-related materials to Boston Harbor.

PubMed

Taylor, David I

2010-04-01

Boston Harbor, a bay-estuary in the north-east USA, has recently been the site of one of the largest wastewater infrastructure projects conducted in the USA, the Boston Harbor Project (BHP). The BHP, which was conducted from 1991 to 2000, ended over a century of direct wastewater treatment facility discharges to the harbor. The BHP caused the loadings of total nitrogen (TN), total phosphorus (TP), total suspended solids (TSS) and particulate organic carbon (POC) to the harbor, to decrease by between 80% and 90%. Approximately one-third of the decreases in TSS and POC loadings occurred between 1991 and 1992; the remaining two-thirds, between 1995 and 2000. For TN and TP, the bulk of the decreases occurred between 1997 or 1998, and 2000. (c) 2009 Elsevier Ltd. All rights reserved.

Which Biomarker is the Best for Predicting Mortality in Incident Peritoneal Dialysis Patients: NT-ProBNP, Cardiac TnT, or hsCRP?

PubMed Central

Oh, Hyung Jung; Lee, Mi Jung; Kwon, Young Eun; Park, Kyoung Sook; Park, Jung Tak; Han, Seung Hyeok; Yoo, Tae-Hyun; Kim, Yong-Lim; Kim, Yon Su; Yang, Chul Woo; Kim, Nam-Ho; Kang, Shin-Wook

2015-01-01

Abstract Although numerous previous studies have explored various biomarkers for their ability to predict mortality in end-stage renal disease (ESRD) patients, these studies have been limited by retrospective analyses, mostly prevalent dialysis patients, and the measurement of only 1 or 2 biomarkers. This prospective study was aimed to evaluate the association between 3 biomarkers and mortality in incident 335 ESRD patients starting continuous ambulatory peritoneal dialysis (CAPD) in Korea. According to the baseline NT-proBNP, cTnT, and hsCRP levels, the patients were stratified into tertiles, and cardiovascular (CV) and all-cause mortalities were compared. Additionally, time-dependent ROC curves were constructed, and the net reclassification index (NRI) and integrated discrimination improvement (IDI) of the models with various biomarkers were calculated. We found the upper tertile of NT-proBNP was significantly associated with increased risk of both CV and all-cause mortalities. However, the upper tertile of hsCRP was significantly related only to the high risk of all-cause mortality even after adjustment for age, sex, and white blood cell counts. Moreover, NT-proBNP had the highest predictive power for CV mortality, whereas hsCRP was the best prognostic marker for all-cause mortality among these biomarkers. In conclusions, NT-proBNP is a more significant prognostic factor for CV mortality than cTnT and hsCRP, whereas hsCRP is a more significant predictor than NT-proBNP and cTnT for all-cause mortality in incident peritoneal dialysis patients. PMID:26554763

Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.

PubMed Central

Monticello, D J; Bakker, D; Schell, M; Finnerty, W R

1985-01-01

Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of mutants that were unable to grow on naphthalene. One such mutant was characterized further. The ability of this mutant to oxidize gentisate was 100-fold less than the ability of a Nah+ Sal+ strain harboring the unmutagenized plasmid, although both strains oxidized and grew on salicylate. These bacteria were presumably able to metabolize salicylate via catechol, since they possessed an inducible, plasmid-encoded catechol 2,3-dioxygenase. Our results suggest that there is an alternate, plasmid-encoded route of salicylate degradation via gentisate and that some plasmid-associated relationship between this pathway and naphthalene oxidation exists. PMID:2988437

Time constraints on the origin of large volume basalts derived from O-isotope and trace element mineral zoning and U-series disequilibria in the Laki and Grímsvötn volcanic system

NASA Astrophysics Data System (ADS)

Bindeman, Ilya N.; Sigmarsson, Olgeir; Eiler, John

2006-05-01

The 1783-1784 AD fissure eruption of Laki (Iceland) produced 15 km 3 of homogeneous basaltic lavas and tephra that are characterized by extreme (3‰) 18O-depletion relative to normal mantle. Basaltic tephra erupted over the last 8 centuries and as late as in November 2004 from the Grímsvötn central volcano, which together with Laki are a part of a single volcanic system, is indistinguishable in δ18O from Laki glass. This suggests that all tap a homogeneous and long-lived low- δ18O magma reservoir. In contrast, we observe extreme oxygen isotope heterogeneity (2.2-5.2‰) in olivine and plagioclase contained within these lavas and tephra, and disequilibrium mineral-glass oxygen-isotope fractionations. Such low- δ18O glass values, and extreme 3‰ range in δ18O olivine have not been described in any other unaltered basalt. The energy constrained mass balance calculation involving oxygen isotopes and major element composition calls for an origin of the Laki-Grímsvötn quartz tholeiitic basaltic melts with δ18O = 3.1‰ by bulk digestion of low- δ18O hydrated basaltic crust with δ18O = - 4‰ to + 1‰, rather than magma mixing with ultra-low- δ18O silicic melt. The abundant Pleistocene hyaloclastites, which were altered by synglacial meltwaters, can serve as a likely assimilant material for the Grímsvötn magmas. The ( 226Ra / 230Th) activity ratio in Laki lavas and 20th century Grímsvötn tephras is 13% in-excess of secular equilibrium, but products of the 20th century Grímsvötn eruptions have equilibrium ( 210Pb / 226Ra). Modeling of oxygen isotope exchange between disequilibrium phenocrysts and magmas, and these short-lived U-series nuclides yields a coherent age for the Laki-Grímsvötn magma reservoir between 100 and 1000 yrs. We propose the existence of uniquely fingerprinted, low- δ18O, homogeneous, large volume, and long-lived basaltic reservoir beneath the Laki-Grímsvötn volcanic system that has been kept alive in its position above the

TN International and ITS operational feedback regarding the decommissioning of obsolete casks dedicated to the transport and/or storage of nuclear raw materials, fuel and used fuel

DOE Office of Scientific and Technical Information (OSTI.GOV)

Blachet, L.; Bimet, F.; Rennesson, N.

2008-07-01

Within the AREVA group, TN International is a major actor regarding the design of casks and transportation for the nuclear cycle. In the early 2005, TN International has started the project of decommissioning some of its own equipment and was hence the first company ever in the AREVA Group to implement this new approach. In order to do so, TN International has based this project by taking into account the AREVA Sustainable Development Charter, the French regulatory framework, the ANDRA (Agence Nationale pour la Gestion des Dechets Radioactifs - National Agency for the radioactive waste management) requirements and has deployedmore » a step by step methodology such as radiological characterization following a logical route. The aim was to define a standardized process with optimized solutions regarding the diversity of the cask's fleet. As a general matter, decommissioning of nuclear casks is a brand new field as the nuclear field is more familiar with the dismantling of nuclear facilities and/or nuclear power plant. Nevertheless existing workshops, maintenance facilities, measurements equipments and techniques have been exploited and adapted by TN International in order to turn an ambitious project into a permanent and cost-effective activity. The decommissioning of the nuclear casks implemented by TN International regarding its own needs and the French regulatory framework is formalized by several processes and is materialized for instance by the final disposal of casks as they are or in ISO container packed with cut-off casks and big bags filled with crushed internal cask equipments, etc. The first part of this paper aims to describe the history of the project that started with a specific environmental analysis which took into account the values of AREVA as regards the Sustainable Development principles that were at the time and are still a topic of current concern in the world. The second part will deal with the definition, the design and the implementation of

The androgen receptor controls expression of the cancer-associated sTn antigen and cell adhesion through induction of ST6GalNAc1 in prostate cancer

PubMed Central

Munkley, Jennifer; Oltean, Sebastian; Vodák, Daniel; Wilson, Brian T.; Livermore, Karen E.; Zhou, Yan; Star, Eleanor; Floros, Vasileios I.; Johannessen, Bjarne; Knight, Bridget; McCullagh, Paul; McGrath, John; Crundwell, Malcolm; Skotheim, Rolf I.; Robson, Craig N.; Leung, Hing Y.; Harries, Lorna W.; Rajan, Prabhakar; Mills, Ian G.; Elliott, David J.

2015-01-01

Patterns of glycosylation are important in cancer, but the molecular mechanisms that drive changes are often poorly understood. The androgen receptor drives prostate cancer (PCa) development and progression to lethal metastatic castration-resistant disease. Here we used RNA-Seq coupled with bioinformatic analyses of androgen-receptor (AR) binding sites and clinical PCa expression array data to identify ST6GalNAc1 as a direct and rapidly activated target gene of the AR in PCa cells. ST6GalNAc1 encodes a sialytransferase that catalyses formation of the cancer-associated sialyl-Tn antigen (sTn), which we find is also induced by androgen exposure. Androgens induce expression of a novel splice variant of the ST6GalNAc1 protein in PCa cells. This splice variant encodes a shorter protein isoform that is still fully functional as a sialyltransferase and able to induce expression of the sTn-antigen. Surprisingly, given its high expression in tumours, stable expression of ST6GalNAc1 in PCa cells reduced formation of stable tumours in mice, reduced cell adhesion and induced a switch towards a more mesenchymal-like cell phenotype in vitro. ST6GalNAc1 has a dynamic expression pattern in clinical datasets, being significantly up-regulated in primary prostate carcinoma but relatively down-regulated in established metastatic tissue. ST6GalNAc1 is frequently upregulated concurrently with another important glycosylation enzyme GCNT1 previously associated with prostate cancer progression and implicated in Sialyl Lewis X antigen synthesis. Together our data establishes an androgen-dependent mechanism for sTn antigen expression in PCa, and are consistent with a general role for the androgen receptor in driving important coordinate changes to the glycoproteome during PCa progression. PMID:26452038

The teratology society 2012-2017 strategic plan: pushing the boundaries.

PubMed

Curran, Christine Perdan; Lau, Christopher; Schellpfeffer, Michael A; Stodgell, Christopher J; Carney, Edward W

2013-01-01

The Teratology Society held its fourth strategic planning session in Albuquerque, NM, April 10-12, 2012, and launched the 2012-2017 Strategic Plan in conjunction with the 2012 annual meeting in Baltimore, MD. Building on the energy of the successful implementation of prior strategic plans (San Diego, 2007; Nashville,TN 2002; Cincinnati, OH 1998), session participants worked to identify barriers to success as a scientific society, as well as impending challenges and opportunities to which the Society needs to respond. The following report provides an overview of the Strategic Planning process, objectives, activities, and conclusions. A total of 23 members were present at the session, and the group included representation from Council, various committees, and different member constituencies. This plan, Pushing the Boundaries, and its three strategic intents: Broaden Our Identity, Expand Our Membership, and Increase Our Influence, will drive the direction of the Teratology Society for the next five years. Copyright © 2013 Wiley Periodicals, Inc.

How Does the Fast Track Intervention Prevent Adverse Outcomes in Young Adulthood?

PubMed

Sorensen, Lucy C; Dodge, Kenneth A

2016-01-01

Numerous studies have shown that childhood interventions can foster improved outcomes in adulthood. Less well understood is precisely how-that is, through which developmental pathways-these interventions work. This study assesses mechanisms by which the Fast Track project (n = 891), a randomized intervention in the early 1990s for high-risk children in four communities (Durham, NC; Nashville, TN; rural PA; and Seattle, WA), reduced delinquency, arrests, and general and mental health service utilization in adolescence through young adulthood (ages 12-20). A decomposition of treatment effects indicates that about a third of Fast Track's impact on later crime outcomes can be accounted for by improvements in social and self-regulation skills during childhood (ages 6-11), such as prosocial behavior, emotion regulation, and problem solving. These skills proved less valuable for the prevention of general and mental health problems. © 2015 The Authors. Child Development © 2015 Society for Research in Child Development, Inc.

Biphasic rattlesnake venom-induced thrombocytopenia.

PubMed

Offerman, Steven R; Barry, J David; Schneir, Aaron; Clark, Richard F

2003-04-01

Thrombocytopenia is a common occurrence in moderate to severe crotaline envenomation. The exact mechanism by which rattlesnake venom leads to thrombocytopenia is unclear, but aggressive treatment with crotaline-specific antivenom often leads to resolution of this disorder. Crotalinae Polyvalent Immune Fab (CroFab(TM), Protherics Inc., Nashville, TN) (crotaline Fab) is now available for the treatment of symptomatic rattlesnake envenomation. Although recurrence of thrombocytopenia has been reported in patients after envenomation treated with crotaline Fab, cases refractory to this therapy have not been described. We report a case of severe crotaline envenomation that appears to have exhibited two separate episodes of thrombocytopenia, only one of which responded to antivenom. The second, later phase was refractory to both crotaline Fab as well as traditional Antivenin (Crotalinae) Polyvalent (Wyeth-Ayerst Pharmaceuticals, Philadelphia, PA) (ACP). By reviewing the literature regarding venom-induced thrombocytopenia, we attempt to explain this "biphasic" phenomenon and the inability of crotaline Fab to reverse this toxic effect.

Development of a GIS-based spill management information system.

PubMed

Martin, Paul H; LeBoeuf, Eugene J; Daniel, Edsel B; Dobbins, James P; Abkowitz, Mark D

2004-08-30

Spill Management Information System (SMIS) is a geographic information system (GIS)-based decision support system designed to effectively manage the risks associated with accidental or intentional releases of a hazardous material into an inland waterway. SMIS provides critical planning and impact information to emergency responders in anticipation of, or following such an incident. SMIS couples GIS and database management systems (DBMS) with the 2-D surface water model CE-QUAL-W2 Version 3.1 and the air contaminant model Computer-Aided Management of Emergency Operations (CAMEO) while retaining full GIS risk analysis and interpretive capabilities. Live 'real-time' data links are established within the spill management software to utilize current meteorological information and flowrates within the waterway. Capabilities include rapid modification of modeling conditions to allow for immediate scenario analysis and evaluation of 'what-if' scenarios. The functionality of the model is illustrated through a case study of the Cheatham Reach of the Cumberland River near Nashville, TN.

Comparison of pre/post-operative CT image volumes to preoperative digitization of partial hepatectomies: a feasibility study in surgical validation

NASA Astrophysics Data System (ADS)

Dumpuri, Prashanth; Clements, Logan W.; Li, Rui; Waite, Jonathan M.; Stefansic, James D.; Geller, David A.; Miga, Michael I.; Dawant, Benoit M.

2009-02-01

Preoperative planning combined with image-guidance has shown promise towards increasing the accuracy of liver resection procedures. The purpose of this study was to validate one such preoperative planning tool for four patients undergoing hepatic resection. Preoperative computed tomography (CT) images acquired before surgery were used to identify tumor margins and to plan the surgical approach for resection of these tumors. Surgery was then performed with intraoperative digitization data acquire by an FDA approved image-guided liver surgery system (Pathfinder Therapeutics, Inc., Nashville, TN). Within 5-7 days after surgery, post-operative CT image volumes were acquired. Registration of data within a common coordinate reference was achieved and preoperative plans were compared to the postoperative volumes. Semi-quantitative comparisons are presented in this work and preliminary results indicate that significant liver regeneration/hypertrophy in the postoperative CT images may be present post-operatively. This could challenge pre/post operative CT volume change comparisons as a means to evaluate the accuracy of preoperative surgical plans.

Image guidance improves localization of sonographically occult colorectal liver metastases

NASA Astrophysics Data System (ADS)

Leung, Universe; Simpson, Amber L.; Adams, Lauryn B.; Jarnagin, William R.; Miga, Michael I.; Kingham, T. Peter

2015-03-01

Assessing the therapeutic benefit of surgical navigation systems is a challenging problem in image-guided surgery. The exact clinical indications for patients that may benefit from these systems is not always clear, particularly for abdominal surgery where image-guidance systems have failed to take hold in the same way as orthopedic and neurosurgical applications. We report interim analysis of a prospective clinical trial for localizing small colorectal liver metastases using the Explorer system (Path Finder Technologies, Nashville, TN). Colorectal liver metastases are small lesions that can be difficult to identify with conventional intraoperative ultrasound due to echogeneity changes in the liver as a result of chemotherapy and other preoperative treatments. Interim analysis with eighteen patients shows that 9 of 15 (60%) of these occult lesions could be detected with image guidance. Image guidance changed intraoperative management in 3 (17%) cases. These results suggest that image guidance is a promising tool for localization of small occult liver metastases and that the indications for image-guided surgery are expanding.

Superfund Record of Decision (EPA Region 4): Milan Army Ammunition Plant, Milan, TN, March 11, 1998

DOE Office of Scientific and Technical Information (OSTI.GOV)

NONE

1998-09-01

This decision document presents the selected action for the Salvage Yard, Former Ammunition Burnout Area (ABA), and Sanitary Landfill at MLAAP, located in Gibson and Carroll Counties, TN. This ROD addresses the final response action planned for the Salvage Yard, Former ABA, and Sanitary Landfill, including soil and groundwater. NFA is the selected remedy for soil and groundwater at the Salvage Yard, Former ABA, and Sanitary landfill. The selected remedy manages the risk to acceptable levels for both human health and the environment and is the final action planned.

Immunization of breast cancer patients using a synthetic sialyl-Tn glycoconjugate plus Detox adjuvant.

PubMed

MacLean, G D; Reddish, M; Koganty, R R; Wong, T; Gandhi, S; Smolenski, M; Samuel, J; Nabholtz, J M; Longenecker, B M

1993-01-01

We have synthesized various formulations that have potential for active specific immunotherapy (ASI) of human cancers. Sialyl-Tn (STn) is a potentially important target structure for ASI because its expression on mucins is a strong, independent predictor of poor prognosis, suggesting that it may have functional significance in the metastatic process. In this first pilot study of synthetic sialyl-Tn hapten conjugated to keyhole limpet hemocyanin (STn-KLH), with Detox adjuvant, toxicity and humoral immunogenicity were assessed in 12 patients with metastatic breast cancer. Toxicity was minimal, restricted to local cutaneous reactions (apart from transient nausea and vomiting following single low-dose cyclophosphamide treatment). Using STn-conjugated human serum albumin in a solid-phase enzyme-linked immunosorbent assay, it was shown that all patients developed IgM and IgG specific for the synthetic STn hapten. Following immunization, most patients were shown to develop increased titres of complement-mediated cytotoxic antibodies, partially inhibited by synthetic STn hapten, but not by the related TF hapten. We also detected IgM and IgG antibodies reactive with natural STn determinants expressed on ovine submaxillary mucin, the STn specificity of this reactivity being confirmed by hapten inhibition. Evaluation of clinical efficacy in a small pilot study is difficult. Five patients are alive 12 or more months after entry, and another 4 patients are alive 6 or more months after entry into the study. All 3 patients with known widespread bulky disease progressed despite ASI, 2 having died from widespread cancer. Two patients had partial responses, each lasting 6 months. While several patients had disease stability for 3-10 months, 1 patient with pulmonary metastases remains stable 15 months after entry into the program.

Linear and exponential TAIL-PCR: a method for efficient and quick amplification of flanking sequences adjacent to Tn5 transposon insertion sites.

PubMed

Jia, Xianbo; Lin, Xinjian; Chen, Jichen

2017-11-02

Current genome walking methods are very time consuming, and many produce non-specific amplification products. To amplify the flanking sequences that are adjacent to Tn5 transposon insertion sites in Serratia marcescens FZSF02, we developed a genome walking method based on TAIL-PCR. This PCR method added a 20-cycle linear amplification step before the exponential amplification step to increase the concentration of the target sequences. Products of the linear amplification and the exponential amplification were diluted 100-fold to decrease the concentration of the templates that cause non-specific amplification. Fast DNA polymerase with a high extension speed was used in this method, and an amplification program was used to rapidly amplify long specific sequences. With this linear and exponential TAIL-PCR (LETAIL-PCR), we successfully obtained products larger than 2 kb from Tn5 transposon insertion mutant strains within 3 h. This method can be widely used in genome walking studies to amplify unknown sequences that are adjacent to known sequences.

Science as a Way of Knowing IV--Developmental Biology. Proceedings of the Annual Meeting (Nashville, TN, December 27-30, 1986).

ERIC Educational Resources Information Center

Moore, John A.; And Others

1987-01-01

This fourth volume of the Science as a Way of Knowing project contains 12 papers given at a conference in 1986 which was cosponsored by a variety of scientific and science education organizations. The presentations include: (1) "Science as a Way of Knowing--Developmental Biology" (John A. Moore); (2) "Embryonic Induction"…

Dissemination and Persistence of blaCTX-M-9 Are Linked to Class 1 Integrons Containing CR1 Associated with Defective Transposon Derivatives from Tn402 Located in Early Antibiotic Resistance Plasmids of IncHI2, IncP1-α, and IncFI Groups

PubMed Central

Novais, Ângela; Cantón, Rafael; Valverde, Aránzazu; Machado, Elisabete; Galán, Juan-Carlos; Peixe, Luísa; Carattoli, Alessandra; Baquero, Fernando; Coque, Teresa M.

2006-01-01

This study analyzes the diversity of In60, a class 1 integron bearing CR1 and containing blaCTX-M-9, and its association with Tn402, Tn21, and classical conjugative plasmids among 45 CTX-M-9-producing clinical strains (41 Escherichia coli strains, 2 Klebsiella pneumoniae strains, 1 Salmonella enterica strain, and 1 Enterobacter cloacae strain). Forty-five patients in a Spanish tertiary care hospital were studied (1996 to 2003). The diversity of In60 and association of In60 with Tn402 or mercury resistance transposons were investigated by overlapping PCR assays and/or hybridization. Plasmid characterization included comparison of restriction fragment length polymorphism patterns and determination of incompatibility group by PCR-based replicon typing, sequencing, and hybridization. CTX-M-9 plasmids belonged to IncHI2 (n = 26), IncP-1α (n = 10), IncFI (n = 4), and IncI (n = 1) groups. Genetic platforms containing blaCTX-M-9 were classified in six types in relation to the In60 backbone and in eight subtypes in relation to Tn402 derivatives. They were associated with Tn21 sequences when located in IncP-1α or IncHI2 plasmids. Our study identified blaCTX-M-9 in a high diversity of CR1-bearing class 1 integrons linked to different Tn402 derivatives, often to Tn21, highlighting the role of recombination events in the evolution of antibiotic resistance plasmids. The presence of blaCTX-M-9 on broad-host-range IncP-1α plasmids might contribute to its dissemination to hosts that were not members of the family Enterobacteriaceae. PMID:16870767

Volcanic ash leaching as a means of tracing the environmental impact of the 2011 Grímsvötn eruption, Iceland.

PubMed

Cabré, J; Aulinas, M; Rejas, M; Fernandez-Turiel, J L

2016-07-01

The Grímsvötn volcanic eruption, from 21 to 28 May, 2011, was the largest eruption of the Grímsvötn Volcanic System since 1873, with a Volcanic Explosivity Index (VEI) of magnitude 4. The main geochemical features of the potential environmental impact of the volcanic ash-water interaction were determined using two different leaching methods as proxies (batch and vertical flow-through column experiments). Ash consists of glass with minor amounts of plagioclase, clinopyroxene, diopside, olivine and iron sulphide; this latter mineral phase is very rare in juvenile ash. Ash grain morphology and size reflect the intense interaction of magma and water during eruption. Batch and column leaching tests in deionised water indicate that Na, K, Ca, Mg, Si, Cl, S and F had the highest potential geochemical fluxes to the environment. Release of various elements from volcanic ash took place immediately through dissolution of soluble salts from the ash surface. Element solubilities of Grímsvötn ash regarding bulk ash composition were <1 %. Combining the element solubilities and the total estimated mass of tephra (7.29 × 10(14) g), the total inputs of environmentally important elements were estimated to be 8.91 × 10(9) g Ca, 7.02 × 10(9) g S, 1.10 × 10(9) g Cl, 9.91 × 10(8) g Mg, 9.91 × 10(8) g Fe and 1.45 × 10(8) g P The potential environmental problems were mainly associated with the release of F (5.19 × 10(9) g).

Effect of the polymer concentration on the ON/OFF states of a TN-LCD: polyvinyl alcohol vs. soy lecithin

NASA Astrophysics Data System (ADS)

de Coss Martinez, Romeo; Gonzalez Murguia, Jose Luis

2011-03-01

In this work we study the response of a Twisted Nematic Liquid Crystal Display (TN-LCD) by varying both the concentration and the polymer used for the microgroove. We compare the performance of two polymers: polyvinyl alcohol and soy lecithin. In particular, the light transmission for the ON/OFF states is evaluated. The polyvinyl alcohol is a polymer widely used in LCDs while lecithin soy is a natural polymer.

Corlew & Perry, Inc. Information Sheet

EPA Pesticide Factsheets

Corlew & Perry, Inc. (the Company) is located in Nashville, Tennessee. The settlement involves renovation activities conducted at a property constructed prior to 1978, located in Nashville, Tennessee.

78 FR 49270 - Information Collection; MyUSA

Federal Register 2010, 2011, 2012, 2013, 2014

2013-08-13

... Floor, Washington, DC 20405- 0001. ATTN: Hada Flowers/IC 3090-00XX; MyUSA. Instructions: Please submit...-4755. ATTN: Hada Flowers/ IC 3090-00xx; MyUSA. Please cite OMB Control No. 3090-XXXX; MyUSA, in all...

Dimensions of religious involvement and leukocyte telomere length.

PubMed

Hill, Terrence D; Ellison, Christopher G; Burdette, Amy M; Taylor, John; Friedman, Katherine L

2016-08-01

Although numerous studies suggest that religious involvement is associated with a wide range of favorable health outcomes, it is unclear whether this general pattern extends to cellular aging. In this paper, we tested whether leukocyte telomere length varies according to several dimensions of religious involvement. We used cross-sectional data from the Nashville Stress and Health Study (2011-2014), a large probability sample of 1252 black and white adults aged 22 to 69 living in Davidson County, TN, USA. Leukocyte telomere length was measured using the monochrome multiplex quantitative polymerase chain reaction method with albumin as the single-copy reference sequence. Dimensions of religious involvement included religiosity, religious support, and religious coping. Our multivariate analyses showed that religiosity (an index of religious attendance, prayer frequency, and religious identity) was positively associated with leukocyte telomere length, even with adjustments for religious support, religious coping, age, gender, race, education, employment status, income, financial strain, stressful life events, marital status, family support, friend support, depressive symptoms, smoking, heavy drinking, and allostatic load. Unlike religiosity, religious support and religious coping were unrelated to leukocyte telomere length across models. Depressive symptoms, smoking, heavy drinking, and allostatic load failed to explain any of the association between religiosity and telomere length. To our knowledge, this is the first population-based study to link religious involvement and cellular aging. Although our data suggest that adults who frequently attend religious services, pray with regularity, and consider themselves to be religious tend to exhibit longer telomeres than those who attend and pray less frequently and do not consider themselves to be religious, additional research is needed to establish the mechanisms underlying this association. Copyright © 2016 Elsevier

Study of the performance of the infrasound Station IS48TN during the period 2011 -2017

NASA Astrophysics Data System (ADS)

Mejri, Chourouk

2017-04-01

The Infrasound Station IS48, in Kesra, Tunisia is part of the verification regime for the Comprehensive Nuclear-Test-Ban Treaty. IS48 is managed and maintained by the Tunisian NDC. Its good location in the middle of the Mediterranean Sea allows to have various and interesting detections. Several events were recorded and identified. But since 2010, the performance of the station has began to be noisy , due to a leakage in the pipe array, despite efforts to solve the issue through the pressure test and maintenance . To this issues, PTS decided to upgrade the WNRS at IS48TN.

Two types of phytases (histidine acid phytase and β-propeller phytase) in Serratia sp. TN49 from the gut of Batocera horsfieldi (coleoptera) larvae.

PubMed

Zhang, Rui; Yang, Peilong; Huang, Huoqing; Shi, Pengjun; Yuan, Tiezheng; Yao, Bin

2011-11-01

Microbial phytases play a major role in the mineralization of organic phosphorous, especially in symbiotic plants and animals. In this study, we identified two types of phytases in Serratia sp. TN49 that was harbored in the gut of Batocera horsfieldi (Coleoptera) larvae. The two phytases, an acidic histidine acid phosphatase (PhyH49) and an alkaline β-propeller phytase (PhyB49), shared low identities with known phytases (61% at most). PhyH49 and PhyB49 produced in Escherichia coli exhibited maximal activities at pH 5.0 (60°C) and pH 7.5-8.0 (45°C), respectively, and are complementary in phytate degradation over the pH range 2.0-9.0. Serratia sp. TN49 harboring both PhyH49 and PhyB49 might make it more adaptive to environment change, corresponding to the evolution trend of microorganism.

Diversity of Tn1546 in vanA-positive Enterococcus faecium clinical isolates with VanA, VanB, and VanD phenotypes and susceptibility to vancomycin.

PubMed

Cha, J O; Yoo, J I; Kim, H K; Kim, H S; Yoo, J S; Lee, Y S; Jung, Y H

2013-10-01

To investigate diversity in the vanA cluster in Enterococcus faecium isolates from nontertiary hospitals. We identified 43 vanA-positive Ent. faecium isolates, including two vancomycin-susceptible isolates, from hospitals between 2003 and 2006. Of these isolates, >85% were resistant to ampicillin, erythromycin and ciprofloxacin. The vanA cluster was classified into six types using overlapping PCR, but the prototype transposon Tn1546 was not found. Most vanA-positive vancomycin-resistant Enterococcus (VRE) carried IS1216V and belonged to Type III (58·1%) or Type II (20·9%). vanY, vanZ and IS1216V were observed in the left and right ends of Type III with long-range PCR. IS1216V was also observed within vanS and vanX in the two vancomycin-susceptible isolates and in two vancomycin-resistant isolates. No VRE isolates with VanB and VanD phenotypes contained point mutations in vanS, unlike in previous reports. Sequence types (STs) of all isolates belonged to clonal complex 17, and ST78 was predominant. Insertion sequences, especially IS1216V, cause structural variation in the vanA cluster. We report the first observation of vanY and vanZ at the left end of Tn1546 in clinical isolates. This is the first report of the frequency of vancomycin resistance and diversity of Tn1546 in vanA-positive Ent. faecium isolates from nontertiary hospitals. © 2013 The Society for Applied Microbiology.

A quantitative real-time PCR assay for the detection of tetR of Tn10 in Escherichia coli using SYBR Green and the Opticon.

PubMed

Morsczeck, Christian; Langendörfer, Daniel; Schierholz, Jörg Michael

2004-06-30

Bacteria of implant infections are extremely resistant to antibiotics. One reason for this antibiotic resistance are transposons; the well-known transposon Tn10, for example, mediates tetracycline resistance to Escherichia coli. Two genes of Tn10, tetA and tetR, are essential for the mechanism of resistance. These genes encode a drug-specific efflux protein and a tetracycline repressor protein, respectively. Tn10 is also widely used in molecular biology. For example, tTA, a recombinant derivate of tetR, has been utilised for a highly efficient gene regulation system in mammalian cells. We have examined E. coli isolates from implant infections for tetracycline resistance and for the presence of tetR. A real-time PCR assay was developed for detection of tetR with SybrGreen using the Opticon PCR machine of MJ Research. This method offers a quick, sensitive, efficient, and reliable approach to the detection and quantification of genes. Clinical isolates of E. coli were examined successfully for tetracycline resistance and for the presence of tetR. The real-time PCR is effective using a variety of templates including isolated E. coli DNA, pure colonies, or liquid culture sources. Using quantified standard DNA, this assay can accurately detect as few as 15 copies. Moreover, this assay has the ability to quantify the number of tetR genes in the presence of contaminating mammalian DNA. In conclusion, the tetR real-time PCR offers new methods for detection and quantification of tetracycline-resistant bacteria and tTA in transfected cell-lines or transgenic animals.

Survival of Escherichia coli with and without ColE1::Tn5 after aerosol dispersal in a laboratory and a farm environment.

PubMed Central

Marshall, B; Flynn, P; Kamely, D; Levy, S B

1988-01-01

The survival of a laboratory strain and a naturally occurring fecal strain of Escherichia coli, with and without a Tn5-containing derivative of ColE1, was examined after aerosol dispersal in a laboratory office and a barn under ambient temperature and humidity conditions. Following the release of paired strains, air and diverse types of surfaces were assayed for the test organisms. In both environments, the number of airborne bacteria declined rapidly within the first 2 h. Longer survival was found on surfaces and varied with surface type: recovery was greatest from wood products. Organisms persisted for 1 day in the office and for up to 20 days in the barn. Survival of the fecal strain was better than that of the laboratory strain in both test environments. In general, plasmid-bearing strains fared similarly to their plasmidless parents, but in several comparisons the ColE1::Tn5-containing strain showed enhanced survival. These studies have implications for the present and proposed release of genetically engineered organisms with and without plasmid vectors. PMID:2843099

Conformational organizations of G-quadruplexes composed of d(G(4)T(n))(3)G(4).

PubMed

Wong, Wan Chi; Zhuang, Jinyi; Ng, Selina Ling Ling; New, Lilian Li Lin; Hiew, Shuhui; Guo, Juanjuan; Yang, Zhaoqi; Li, Tianhu

2010-08-01

Structural polymorphism is one of the important issues with regard to G-quadruplexes because the structural diversity may significantly affect their biological functions in vivo and their physical property in nano-material. A series of oligonucleotides with four repeat guanines sequence [d(G(4)T(n))(3)G(4) (n=1-6)] were designed. In this study, the effects of loop length on the formation of structures of G-quadruplex were investigated through the result of CD (circular dichroism) and 20% non-denatured polyacrylamide gel electrophoresis. Our studies demonstrate that the length of loop in 100mM KCl solution could predict the conformation of G-quadruplex. Copyright 2010 Elsevier Ltd. All rights reserved.

Water-quality assessment of the Lower Grand River Basin, Missouri and Iowa, USA, in support of integrated conservation practices

USGS Publications Warehouse

Wilkison, Donald H.; Armstrong, Daniel J.

2016-01-01

The effectiveness of agricultural conservation programmes to adequately reduce nutrient exports to receiving streams and to help limit downstream hypoxia issues remains a concern. Quantifying programme success can be difficult given that short-term basin changes may be masked by long-term water-quality shifts. We evaluated nutrient export at stream sites in the 44 months that followed a period of increased, integrated conservation implementation within the Lower Grand River Basin. These short-term responses were then compared with export that occurred in the main stem and adjacent rivers in northern Missouri over a 22-year period to better contextualize any recent changes. Results indicate that short-term (October 2010 through May 2014) total nitrogen (TN) concentrations in the Grand River were 20% less than the long-term average, and total phosphorus (TP) concentrations were 23% less. Nutrient reductions in the short term were primarily the result of the less-than-average precipitation and, consequently, streamflow that was 36% below normal. Therefore, nutrient concentrations measured in tributary streams were likely less than normal during the implementation period. Northern Missouri streamflow-normalized TN concentrations remained relatively flat or declined over the period 1991 through 2013 likely because available sources of nitrogen, determined as the sum of commercial fertilizers, available animal manures and atmospheric inputs, were typically less than crop requirement for much of that time frame. Conversely, flow-normalized stream TP concentrations increased over the past 22 years in northern Missouri streams, likely in response to many years of phosphorus inputs in excess of crop requirements. Stream nutrient changes were most pronounced during periods that coincided with the major tillage, planting and growth phases of row crops and increased streamflow. Nutrient reduction strategies targeted at the period February through June would likely have the

Tn-seq of Caulobacter crescentus under uranium stress reveals genes essential for detoxification and stress tolerance

DOE PAGES

Yung, Mimi C.; Park, Dan M.; Overton, K. Wesley; ...

2015-07-20

Ubiquitous aquatic bacterium Caulobacter crescentus is highly resistant to uranium (U) and facilitates U biomineralization and thus holds promise as an agent of U bioremediation. In order to gain an understanding of how C. crescentus tolerates U, we employed transposon (Tn) mutagenesis paired with deep sequencing (Tn-seq) in a global screen for genomic elements required for U resistance. Of the 3,879 annotated genes in the C. crescentus genome, 37 were found to be specifically associated with fitness under U stress, 15 of which were subsequently tested through mutational analysis. Systematic deletion analysis revealed that mutants lacking outer membrane transporters (rsaFamore » and rsaFb), a stress-responsive transcription factor (cztR), or a ppGpp synthetase/hydrolase (spoT) exhibited a significantly lower survival rate under U stress. RsaFa and RsaFb, which are homologues of TolC in Escherichia coli, have previously been shown to mediate S-layer export. Transcriptional analysis revealed upregulation of rsaF a and rsaF b by 4- and 10-fold, respectively, in the presence of U. We additionally show that rsaFa mutants accumulated higher levels of U than the wild type, with no significant increase in oxidative stress levels. These results suggest a function for RsaF a and RsaF b in U efflux and/or maintenance of membrane integrity during U stress. In addition, we present data implicating CztR and SpoT in resistance to U stress. Together, our findings reveal novel gene targets that are key to understanding the molecular mechanisms of U resistance in C. crescentus.« less

2012 ESTCP Live Site Demonstrations, Spencer, TN. ESTCP MR-1165 Demonstration Data Report, Former Spencer Artillery Range TEMTADS Demonstration

DTIC Science & Technology

2013-05-29

Victoria Kantsios, 2 Glenn R. Harbaugh,3 and Daniel A. Steinhurst3 Naval Research Laboratory, Code 6110 4555 Overlook Avenue, SW Washington, DC 20375...Unclassified Unlimited 72 B.J. Spargo, NRL, Code 6110 (202) 404-6392 The TEMTADS 5×5 Array and TEMTADS MP 2 × 2 Cart were demonstrated at the former...Naval Research Laboratory Washington, DC 20375-5320 NRL/MR/ 6110 --13-9470 2012 ESTCP Live Site Demonstrations Spencer, TN ESTCP MR-1165 Demonstration

National assessment of Tree City USA participation

EPA Pesticide Factsheets

Tree City USA is a national program that recognizes municipal commitment to community forestry. In return for meeting program requirements, Tree City USA participants expect social, economic, and/or environmental benefits. Understanding the geographic distribution and socioeconomic characteristics of Tree City USA communities at the national scale can offer insights into the motivations or barriers to program participation, and provide context for community forestry research at finer scales. In this study, researchers assessed patterns in Tree City USA participation for all U.S. communities with more than 2,500 people according to geography, community population size, and socioeconomic characteristics, such as income, education, and race. Nationally, 23.5% of communities studied were Tree City USA participants, and this accounted for 53.9% of the total population in these communities. Tree City USA participation rates varied substantially by U.S. region, but in each region participation rates were higher in larger communities, and long-term participants tended to be larger communities than more recent enrollees. In logistic regression models, owner occupancy rates were significant negative predictors of Tree City USA participation, education and percent white population were positive predictors in many U.S. regions, and inconsistent patterns were observed for income and population age. The findings indicate that communities with smaller populations, lower educat

Association of Genome-Wide Variation with Highly Sensitive Cardiac Troponin-T (hs-cTnT) Levels in European- and African-Americans: A Meta-Analysis from the Atherosclerosis Risk in Communities and the Cardiovascular Health Studies

PubMed Central

Yu, Bing; Barbalic, Maja; Brautbar, Ariel; Nambi, Vijay; Hoogeveen, Ron C.; Tang, Weihong; Mosley, Thomas H.; Rotter, Jerome I.; deFilippi, Christopher R.; O’Donnell, Christopher J.; Kathiresan, Sekar; Rice, Ken; Heckbert, Susan R.; Ballantyne, Christie M.; Psaty, Bruce M.; Boerwinkle, Eric

2013-01-01

Background High levels of cardiac troponin T measured by a highly sensitive assay (hs-cTnT) are strongly associated with incident coronary heart disease (CHD) and heart failure (HF). No large-scale genome-wide association study (GWAS) of hs-cTnT has been reported to date. We sought to identify novel genetic variants that are associated with hs-cTnT levels. Methods and Results We performed a GWAS in 9,491 European-Americans and 2,053 African-Americans free of CHD and HF from 2 prospective cohorts: the Atherosclerosis Risk in Communities Study (ARIC) and the Cardiovascular Health Study (CHS). GWASs were conducted in each study and race stratum. Fixed-effect meta-analyses combined the results of linear regression from 2 cohorts within each race stratum, and then across race strata to produce overall estimates and p-values. The meta-analysis identified a significant association at chromosome 8q13 (rs10091374, p = 9.06 × 10−9) near the nuclear receptor coactivator 2 (NCOA2) gene. Over-expression of NCOA2 can be detected in myoblasts An additional analysis using logistic regression and the clinically motivated 99th percentile cut-point detected a significant association at 1q32 (rs10091374, p = 9.06 × 10−8) in the gene TNNT2, which encodes the cardiac troponin T protein itself. The hs-cTnT-associated SNPs were not associated with CHD in a large case-control study, but rs12564445 was significantly associated with incident HF in ARIC European-Americans (HR = 1.16, p-value = 0.004). Conclusions We identified 2 loci, near NCOA2 and in the TNNT2 gene, at which variation was significantly associated with hs-cTnT levels. Further use of the new assay should enable replication of these results. PMID:23247143

USA National Phenology Network gridded products documentation

USGS Publications Warehouse

Crimmins, Theresa M.; Marsh, R. Lee; Switzer, Jeff R.; Crimmins, Michael A.; Gerst, Katharine L.; Rosemartin, Alyssa H.; Weltzin, Jake F.

2017-02-23

The goals of the USA National Phenology Network (USA-NPN, www.usanpn.org) are to advance science, inform decisions, and communicate and connect with the public regarding phenology and species’ responses to environmental variation and climate change. The USA-NPN seeks to facilitate informed ecosystem stewardship and management by providing phenological information freely and openly. One way the USA-NPN is endeavoring to accomplish these goals is by providing data and data products in a wide range of formats, including gridded real-time, short-term forecasted, and historical maps of phenological events, patterns and trends. This document describes the suite of gridded phenologically relevant data products produced and provided by the USA National Phenology Network, which can be accessed at www.usanpn.org/data/phenology_maps and also through web services at geoserver.usanpn.org/geoserver/wms?request=GetCapabilities.

Effects of short-term tocopherol (T) feeding on structure-localized protein tyrosine nitration (pTN) patterns of mitochondrial ATPase following endotoxin (LPS) challenge in beef calves.

USDA-ARS?s Scientific Manuscript database

Mitochondrial ATPase/Complex-V (MCV) is an electron transport chain (ETC) component needed for ATP synthesis. The ETC, exquisitely sensitive to proinflammatory mediators (PIM), generates oxynitrogen reactants leading to pTN formation as mitochondrial membrane leakage occurs. Immunohistochemical loca...

Postoperative change of anti-Thomsen-Friedenreich and Tn IgG level: the follow-up study of gastrointestinal cancer patients.

PubMed

Smorodin, Eugeniy-P; Kurtenkov, Oleg-A; Sergeyev, Boris-L; Kodar, Kristel-E; Chuzmarov, Valentin-I; Afanasyev, Vladimir-P

2008-07-21

To study the influence of tumor removal on the serum level of IgG antibodies to tumor-associated Thomsen-Friedenreich (TF), Tn carbohydrate epitopes and xenogeneic alphaGal, and to elucidate on the change of the level during the follow-up as well as its association with the stage and morphology of the tumor and the values of blood parameters in gastrointestinal cancer. Sixty patients with gastric cancer and 34 patients with colorectal cancer in stages I-IV without distant metastases were subjected to follow-up. The level of antibodies in serum was determined by the enzyme-linked immunosorbent assay (ELISA) using synthetic polyacrylamide (PAA) glycoconjugates. Biochemical and haematological analyses were performed using automated equipment. In gastrointestinal cancer, the TF antibody level was found to have elevated significantly after the removal of G3 tumors as compared with the preoperative level (u = 278.5, P < 0.05). After surgery, the TF and Tn antibody level was elevated in the majority of gastric cancer patients (sign test, 20 vs 8, P < 0.05, and 21 vs 8, P < 0.05, respectively). In gastrointestinal cancer, the elevated postoperative level of TF, Tn and alphaGal antibodies was noted in most patients with G3 tumors (sign test, 22 vs 5, P < 0.01; 19 vs 6, P < 0.05; 24 vs 8, P < 0.01, respectively), but the elevation was not significant in patients with G1 + G2 resected tumors. The postoperative follow-up showed that the percentage of patients with G3 resected tumors of the digestive tract, who had a mean level of anti-TF IgG above the cut-off value (1.53), was significantly higher than that of patients with G1 + G2 resected tumors (c2 = 3.89, all patients; c2 = 5.34, patients without regional lymph node metastases; P < 0.05). The percentage of patients with a tumor in stage I, whose mean anti-TF IgG level remained above the cut-off value (1.26), was significantly higher than that of patients with the cancer in stages III-IV (c2 = 4.71, gastric cancer; c2 = 4

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

Attendees of the USA Science and Engineering Festival explore all of the exhibits at the NASA Stage. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

TnBP⁄Triton X-45 Treatment of Plasma for Transfusion Efficiently Inactivates Hepatitis C Virus

PubMed Central

Chou, Ming-Li; Burnouf, Thierry; Chang, Shun-Pang; Hung, Ting-Chun; Lin, Chun-Ching; Richardson, Christopher D.; Lin, Liang-Tzung

2015-01-01

Risk of transmission of hepatitis C virus (HCV) by clinical plasma remains high in countries with a high prevalence of hepatitis C, justifying the implementation of viral inactivation treatments. In this study, we assessed the extent of inactivation of HCV during minipool solvent/detergent (SD; 1% TnBP / 1% Triton X-45) treatment of human plasma. Luciferase-tagged infectious cell culture-derived HCV (HCVcc) particles were used to spike human plasma prior to treatment by SD at 31 ± 0.5°C for 30 min. Samples were taken before and after SD treatment and filtered on a Sep-Pak Plus C18 cartridge to remove the SD agents. Risk of cytotoxicity was assessed by XTT cell viability assay. Viral infectivity was analyzed based on the luciferase signals, 50% tissue culture infectious dose viral titer, and immunofluorescence staining for HCV NS5A protein. Total protein, cholesterol, and triglyceride contents were determined before and after SD treatment and C18 cartridge filtration. Binding analysis, using patient-derived HCV clinical isolates, was also examined to validate the efficacy of the inactivation by SD. SD treatment effectively inactivated HCVcc within 30 min, as demonstrated by the baseline level of reporter signals, total loss of viral infectivity, and absence of viral protein NS5A. SD specifically targeted HCV particles to render them inactive, with essentially no effect on plasma protein content and hemostatic function. More importantly, the efficacy of the SD inactivation method was confirmed against various genotypes of patient-derived HCV clinical isolates and against HCVcc infection of primary human hepatocytes. Therefore, treatment by 1% TnBP / 1% Triton X-45 at 31°C is highly efficient to inactivate HCV in plasma for transfusion, showing its capacity to enhance the safety of therapeutic plasma products. We propose that the methodology used here to study HCV infectivity can be valuable in the validation of viral inactivation and removal processes of human

DOE Office of Scientific and Technical Information (OSTI.GOV)

Yee, Ben Chung; Wollaber, Allan Benton; Haut, Terry Scot

The high-order low-order (HOLO) method is a recently developed moment-based acceleration scheme for solving time-dependent thermal radiative transfer problems, and has been shown to exhibit orders of magnitude speedups over traditional time-stepping schemes. However, a linear stability analysis by Haut et al. (2015 Haut, T. S., Lowrie, R. B., Park, H., Rauenzahn, R. M., Wollaber, A. B. (2015). A linear stability analysis of the multigroup High-Order Low-Order (HOLO) method. In Proceedings of the Joint International Conference on Mathematics and Computation (M&C), Supercomputing in Nuclear Applications (SNA) and the Monte Carlo (MC) Method; Nashville, TN, April 19–23, 2015. American Nuclear Society.)more » revealed that the current formulation of the multigroup HOLO method was unstable in certain parameter regions. Since then, we have replaced the intensity-weighted opacity in the first angular moment equation of the low-order (LO) system with the Rosseland opacity. Furthermore, this results in a modified HOLO method (HOLO-R) that is significantly more stable.« less

A stable 1D multigroup high-order low-order method

DOE PAGES

Yee, Ben Chung; Wollaber, Allan Benton; Haut, Terry Scot; ...

2016-07-13

The high-order low-order (HOLO) method is a recently developed moment-based acceleration scheme for solving time-dependent thermal radiative transfer problems, and has been shown to exhibit orders of magnitude speedups over traditional time-stepping schemes. However, a linear stability analysis by Haut et al. (2015 Haut, T. S., Lowrie, R. B., Park, H., Rauenzahn, R. M., Wollaber, A. B. (2015). A linear stability analysis of the multigroup High-Order Low-Order (HOLO) method. In Proceedings of the Joint International Conference on Mathematics and Computation (M&C), Supercomputing in Nuclear Applications (SNA) and the Monte Carlo (MC) Method; Nashville, TN, April 19–23, 2015. American Nuclear Society.)more » revealed that the current formulation of the multigroup HOLO method was unstable in certain parameter regions. Since then, we have replaced the intensity-weighted opacity in the first angular moment equation of the low-order (LO) system with the Rosseland opacity. Furthermore, this results in a modified HOLO method (HOLO-R) that is significantly more stable.« less

77 FR 76006 - Star Networks USA, LLC; Complaint

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-26

... CONSUMER PRODUCT SAFETY COMMISSION [CPSC Docket No. 13-2] Star Networks USA, LLC; Complaint AGENCY.... Published below is a Complaint: In the Matter of Star Networks USA, LLC.\\1\\ \\1\\ Chairman Inez M. Tenenbaum... COMMISSION In the Matter of STAR NETWORKS USA, LLC, Respondent CPSC DOCKET NO. 13-2 COMPLAINT Nature of...

77 FR 27082 - StarTek USA, Inc., 244 Dundee Avenue, Greeley, CO; StarTek USA, Inc., 1250 H Street, Greeley, CO...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-08

... DEPARTMENT OF LABOR Employment and Training Administration [TA-W-74,850; TA-W-74,850A] StarTek USA, Inc., 244 Dundee Avenue, Greeley, CO; StarTek USA, Inc., 1250 H Street, Greeley, CO; Amended... workers and former workers of StarTek USA, Inc., Greeley, Colorado. The Department's notice of...

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

An attendee of the USA Science and Engineering Festival uses marbles to build a universe consisting of atoms and dark matter. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

USA National Phenology Network observational data documentation

USGS Publications Warehouse

Rosemartin, Alyssa H.; Denny, Ellen G.; Gerst, Katharine L.; Marsh, R. Lee; Posthumus, Erin E.; Crimmins, Theresa M.; Weltzin, Jake F.

2018-04-25

The goals of the USA National Phenology Network (USA-NPN, www.usanpn.org) are to advance science, inform decisions, and communicate and connect with the public regarding phenology and species’ responses to environmental variation and climate change. The USA-NPN seeks to advance the science of phenology and facilitate ecosystem stewardship by providing phenological information freely and openly. To accomplish these goals, the USA-NPN National Coordinating Office (NCO) delivers observational data on plant and animal phenology in several formats, including minimally processed status and intensity datasets and derived phenometrics for individual plants, sites, and regions. This document describes the suite of observational data products delivered by the USA National Phenology Network, covering the period 2009–present for the United States and accessible via the Phenology Observation Portal (http://dx.doi.org/10.5066/F78S4N1V) and via an Application Programming Interface. The data described here have been used in diverse research and management applications, including over 30 publications in fields such as remote sensing, plant evolution, and resource management.

75 FR 62428 - Investigations Regarding Certifications of Eligibility To Apply for Worker Adjustment Assistance

Federal Register 2010, 2011, 2012, 2013, 2014

2010-10-08

..., courier services, or mail, to FOIA Disclosure Officer, Office of Trade Adjustment Assistance (ETA), U.S.../10 09/02/10 (Company). 74618 Young's Furniture Whitesburg, TN........ 09/13/10 09/09/10 Manufacturing Company, Inc., (Company). 74619 Sematic USA, Inc., Twinsburg, OH......... 09/13/10 08/16/10 (Workers...

Assessment of Regeneration of Bone in the Extracted Third Molar Sockets Augmented Using Xenograft (CollaPlugTN Zimmer) in Comparison with the Normal Healing on the Contralateral Side.

PubMed

Ranganathan, Murugan; Balaji, M; Krishnaraj, R; Narayanan, Vivek; Thangavelu, Annamalai

2017-11-01

Alveolar bone resorption is a significant clinical problem. Bone loss in third molar region following extraction or surgical removal not only leads to periodontal problems in second molar region but also it may lead to some serious problems like increased incidence of angle fractures. In order to reduce the risks following third molar surgery, the socket should be augmented with bone grafts. In recent days guided tissue regeneration is the most accepted and successful technique followed many authors and its efficacy has been proved. Based upon our clinical experience, the use of bio absorbable collagen wound dressing such as CollaPlug TN has achieved quick healing and more primary wound coverage. Amongst the graft materials collagen is preferable due to its high biocompatibility and hemostatic ability. This study was done to assess the regeneration of bone in the extracted third molar sockets using xenograft (CollaPlug TN -Zimmer) which was compared with the normal healing on the contra lateral side. The assessment was done to analyze post-operative healing complications and to compare the bone density formed between control site and implant site radiologically. On this basis of this study, the use of collaplugTN appears to be beneficial to the patient in postoperative wound healing and also for better bone formation. The use of this material was advantageous because of its simplicity of application cost effectiveness and availability. There is enhanced wound healing and early bone formation.

Physicochemical and toxicological profiling of ash from the 2010 and 2011 eruptions of Eyjafjallajökull and Grímsvötn volcanoes, Iceland using a rapid respiratory hazard assessment protocol.

PubMed

Horwell, C J; Baxter, P J; Hillman, S E; Calkins, J A; Damby, D E; Delmelle, P; Donaldson, K; Dunster, C; Fubini, B; Kelly, F J; Le Blond, J S; Livi, K J T; Murphy, F; Nattrass, C; Sweeney, S; Tetley, T D; Thordarson, T; Tomatis, M

2013-11-01

The six week eruption of Eyjafjallajökull volcano in 2010 produced heavy ash fall in a sparsely populated area of southern and south eastern Iceland and disrupted European commercial flights for at least 6 days. We adopted a protocol for the rapid analysis of volcanic ash particles, for the purpose of informing respiratory health risk assessments. Ash collected from deposits underwent a multi-laboratory physicochemical and toxicological investigation of their mineralogical parameters associated with bio-reactivity, and selected in vitro toxicology assays related to pulmonary inflammatory responses. Ash from the eruption of Grímsvötn, Iceland, in 2011 was also studied. The results were benchmarked against ash from Soufrière Hills volcano, Montserrat, which has been extensively studied since the onset of eruptive activity in 1995. For Eyjafjallajökull, the grain size distributions were variable: 2-13 vol% of the bulk samples were <4 µm, with the most explosive phases of the eruption generating abundant respirable particulate matter. In contrast, the Grímsvötn ash was almost uniformly coarse (<3.5 vol%<4 µm material). Surface area ranged from 0.3 to 7.7 m2 g(-1) for Eyjafjallajökull but was very low for Grímsvötn (<0.6 m2 g(-1)). There were few fibre-like particles (which were unrelated to asbestos) and the crystalline silica content was negligible in both eruptions, whereas Soufrière Hills ash was cristobalite-rich with a known potential to cause silicosis. All samples displayed a low ability to deplete lung antioxidant defences, showed little haemolysis and low acute cytotoxicity in human alveolar type-1 like epithelial cells (TT1). However, cell-free tests showed substantial hydroxyl radical generation in the presence of hydrogen peroxide for Grímsvötn samples, as expected for basaltic, Fe-rich ash. Cellular mediators MCP-1, IL-6, and IL-8 showed chronic pro-inflammatory responses in Eyjafjallajökull, Grímsvötn and Soufrière Hills samples

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

A NASA staff member shows attendees of the USA Science and Engineering Festival what happens to the human body in space without a space suit using a marshmallow bunny. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

Religious Socialization and Benevolence: A Study among American Pentecostals

ERIC Educational Resources Information Center

Cartledge, Mark J.

2013-01-01

This study explores benevolent action among Pentecostals in the USA using a congregational questionnaire survey of worshippers among the Church of God (Cleveland, TN) (N = 1522). The influence of religiosity and the socialization of a perceived relationship of love with God (Godly Love) are explored, while controlling for background variables. The…

Ionothermal synthesis of discrete supertetrahedral Tn (n = 4, 5) clusters with tunable components, band gaps, and fluorescence properties.

PubMed

Yang, Dan-Dan; Li, Wei; Xiong, Wei-Wei; Li, Jian-Rong; Huang, Xiao-Ying

2018-05-01

The preparation of crystalline molecularly supertetrahedral Tn clusters with variable sizes and components is of vital importance for the fundamental study of their physicochemical properties. However, setting up an efficient method to stabilize large discrete Tn clusters is a challenge due to their high negative charges and polymerization nature. In this work, we report on the ionothermal synthesis of three discrete T4 cluster compounds, namely [Bmmim]5[(CH3)2NH2]4[NH4][M4In16S31(SH)4]·6H2O (M = Mn (1), Zn (2), Cd (3), Bmmim = 1-buty-2,3-dimethyl-imidazolium), and four discrete T5 cluster compounds, namely [Bmmim]10[NH4]3[Cu5Ga30-xInxS52(SH)4] (x = 6.6 (5), 14.5 (6), 23.8 (7), and 30 (8)). The compound [Bmmim]10[NH4]3[Cu5Ga30S52(SH)4] (4) previously reported by us features a discrete T5 cluster. The steep UV-Vis absorption edges indicate band gaps of 2.20 eV for 1, 2.64 eV for 2, 2.69 eV for 3, 3.04 eV for 4, 2.65 eV for 5, 2.48 eV for 6, 2.32 eV for 7, and 2.30 eV for 8. The compositions of T5 clusters could be varied with the ratios of Ga : In in the starting reagents, providing an opportunity to systematically control the band gaps and fluorescence performances of T5 cluster-based compounds. This research might advance the understanding of the ionothermal preparation and functionality tuning of crystalline chalcogenides.

Diesel Fuel Processing for the PAFC Process Demonstration.

DTIC Science & Technology

1986-12-15

c M-0 ca W ’ U 141 _ ____ _ _ _ __ _ _ 104 ENERGY RESEARCH CORPORATION ??~ Ŕ, E 6/17/86 Exxon Company, USA 200 Unicorn Park Woburn, Mass. 01801-3390...Sincerely, George Steinfeld Program Manager 105 , E ON COMPANY U.S.A. 200 UNICORN PARK • AOBURN, MASSACHUSE S0801-3390 M %A’KE ,DEPARTVENT T .’AQKE7N...Fort Myer Drive rTN: RFP Control-Corp. Marketing .*. Arlington, VA 22209 Bldg C2 Mail Station B199 P.O. Box 1053 El Segundo, CA 90245 Cbmmander (12

Annual Performance Evaluation of a Pair of Energy Efficient Houses (WC3 and WC4) in Oak Ridge, TN

DOE Office of Scientific and Technical Information (OSTI.GOV)

Biswas, Kaushik; Christian, Jeffrey E; Gehl, Anthony C

2012-04-01

Beginning in 2008, two pairs of energy-saver houses were built at Wolf Creek in Oak Ridge, TN. These houses were designed to maximize energy efficiency using new ultra-high-efficiency components emerging from ORNL s Cooperative Research and Development Agreement (CRADA) partners and others. The first two houses contained 3713 square feet of conditioned area and were designated as WC1 and WC2; the second pair consisted of 2721 square feet conditioned area with crawlspace foundation and they re called WC3 and WC4. This report is focused on the annual energy performance of WC3 and WC4, and how they compare against a previouslymore » benchmarked maximum energy efficient house of a similar footprint. WC3 and WC4 are both about 55-60% more efficient than traditional new construction. Each house showcases a different envelope system: WC3 is built with advanced framing featured cellulose insulation partially mixed with phase change materials (PCM); and WC4 house has cladding composed of an exterior insulation and finish system (EIFS). The previously benchmarked house was one of three built at the Campbell Creek subdivision in Knoxville, TN. This house (CC3) was designed as a transformation of a builder house (CC1) with the most advanced energy-efficiency features, including solar electricity and hot water, which market conditions are likely to permit within the 2012 2015 period. The builder house itself was representative of a standard, IECC 2006 code-certified, all-electric house built by the builder to sell around 2005 2008.« less

Evidence-Based Redesign of the COMLEX-USA Series.

PubMed

Gimpel, John R; Horber, Dorothy; Sandella, Jeanne M; Knebl, Janice A; Thornburg, John E

2017-04-01

To ensure that the Comprehensive Osteopathic Medical Licensing Examination-USA (COMLEX-USA) reflects the evolving practice of osteopathic medicine, the National Board of Osteopathic Medical Examiners has developed new content and format specifications for an enhanced, competency-based examination program to be implemented with COMLEX-USA Level 3 in 2018. This article summarizes the evidence-based design processes that served as the foundation for blueprint development and the evidence supporting its validity. An overview is provided of the blueprint's 2 dimensions: Competency Domains and Clinical Presentations. The authors focus on the evidence that supports interpretation of test scores for the primary and intended purpose of COMLEX-USA, which is osteopathic physician licensure. Important secondary uses and the educational and catalytic effect of assessments are also described. This article concludes with the National Board of Osteopathic Medical Examiners' plans to ensure that the COMLEX-USA series remains current and meets the needs of its stakeholders-the patients who seek care from osteopathic physicians.

Brownfield to Brightfield Initiative in Oak Ridge, TN - 12346

DOE Office of Scientific and Technical Information (OSTI.GOV)

Hough, Gil; Fairless, Chad

Experience characterizing, permitting, and restoring 'Brownfield' sites-government or industrial sites with restricted future use due to the presence or potential presence of hazardous substances, pollutants, or contaminants-is being leveraged to identify opportunities for redevelopment into solar power generating facilities which, in this context, are called 'Brightfields'. Brownfield sites offer the expansive land necessary for large photovoltaic (PV) solar farms, but require an in-depth working knowledge of complicated regulatory restrictions and environmental constraints to develop them. As a part of the effort to identify opportunities for redevelopment of Brownfield sites for solar applications, a technical guide, was composed specifically for themore » development of solar generation on restricted use sites. The basis of the technical guide gives specific consideration to environmental requirements and installation methods breaking that into three areas for assessing: 1) levels of contamination, 2) ground penetration requirements, and 3) the requirements for aesthetics and maintenance. Brightfield projects are underway to support the technical guide and expand re-industrialization efforts for the former DOE Gaseous Diffusion Plant in Oak Ridge, TN. There are exciting opportunities to turn Brownfields into Brightfield solar energy solutions for meeting the future renewable energy needs of our country. Brownfields that offer the large surface area required for solar PV farms coupled with the technical guide for the installation of solar farms on restricted use sites supports efforts to develop the solar capacities and expertise to tap this future market. The initial projects designed following the technical guide will provide verification of the installation requirements and beneficial reuse of restricted use sites. (authors)« less

76 FR 35715 - Establishment of the SelectUSA Initiative

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-20

... Establishment of the SelectUSA Initiative By the authority vested in me as President by the Constitution and the... unnecessary obstacles to investment. Sec. 2. SelectUSA Initiative. (a) Establishment. There is established the SelectUSA Initiative (Initiative), a Government-wide initiative to attract and retain investment in the...

Volcano Deformation and Eruption Forecasting using Data Assimilation: Case of Grimsvötn volcano in Iceland

NASA Astrophysics Data System (ADS)

Bato, Mary Grace; Pinel, Virginie; Yan, Yajing

2016-04-01

The recent advances in Interferometric Synthetic Aperture Radar (InSAR) imaging and the increasing number of continuous Global Positioning System (GPS) networks recorded on volcanoes provide continuous and spatially extensive evolution of surface displacements during inter-eruptive periods. For basaltic volcanoes, these measurements combined with simple dynamical models (Lengliné et al. 2008 [1], Pinel et al, 2010 [2], Reverso et al, 2014 [3]) can be exploited to characterise and constrain parameters of one or several magmatic reservoirs using inversion methods. On the other hand, data assimilation-a time-stepping process that best combines models and observations, sometimes a priori information based on error statistics to predict the state of a dynamical system-has gained popularity in various fields of geoscience (e.g. ocean-weather forecasting, geomagnetism and natural resources exploration). In this work, we aim to first test the applicability and benefit of data assimilation, in particular the Ensemble Kalman Filter [4], in the field of volcanology. We predict the temporal behaviors of the overpressures and deformations by applying the two-magma chamber model of Reverso et. al., 2014 [3] and by using synthetic deformation data in order to establish our forecasting strategy. GPS time-series data of the recent eruptions at Grimsvötn volcano is used for the real case applicability of the method. [1] Lengliné, O., D Marsan, J Got, V. Pinel, V. Ferrazzini, P. Obuko, Seismicity and deformation induced by magma accumulation at three basaltic volcanoes, J. Geophys. Res., 113, B12305, 2008. [2] V. Pinel, C. Jaupart and F. Albino, On the relationship between cycles of eruptive activity and volcanic edifice growth, J. Volc. Geotherm. Res, 194, 150-164, 2010 [3] T. Reverso, J. Vandemeulebrouck, F. Jouanne, V. Pinel, T. Villemin, E. Sturkell, A two-magma chamber as a source of deformation at Grimsvötn volcano, Iceland, JGR, 2014 [4] Evensen, G., The Ensemble Kalman

Incremental Aerodynamic Coefficient Database for the USA2

NASA Technical Reports Server (NTRS)

Richardson, Annie Catherine

2016-01-01

In March through May of 2016, a wind tunnel test was conducted by the Aerosciences Branch (EV33) to visually study the unsteady aerodynamic behavior over multiple transition geometries for the Universal Stage Adapter 2 (USA2) in the MSFC Aerodynamic Research Facility's Trisonic Wind Tunnel (TWT). The purpose of the test was to make a qualitative comparison of the transonic flow field in order to provide a recommended minimum transition radius for manufacturing. Additionally, 6 Degree of Freedom force and moment data for each configuration tested was acquired in order to determine the geometric effects on the longitudinal aerodynamic coefficients (Normal Force, Axial Force, and Pitching Moment). In order to make a quantitative comparison of the aerodynamic effects of the USA2 transition geometry, the aerodynamic coefficient data collected during the test was parsed and incorporated into a database for each USA2 configuration tested. An incremental aerodynamic coefficient database was then developed using the generated databases for each USA2 geometry as a function of Mach number and angle of attack. The final USA2 coefficient increments will be applied to the aerodynamic coefficients of the baseline geometry to adjust the Space Launch System (SLS) integrated launch vehicle force and moment database based on the transition geometry of the USA2.

Role of Inflammation in MPTP-Induced Dopaminergic Degeneration

DTIC Science & Technology

2005-12-01

8217Department of Developmental Neurohiolog/, Saint Jude Children k Research Hospital, Memphis, TN 39105. USA "tNeuroscience Research Laboratories qf...astrucytes. J. Neuror’heo,. 68. 2216-22)9. Lannsion 1WV, LS Porno . J Te~trud, AG Reeves. JA Kaplan and 1) Lichent C J Arsaur. [R Vaulter and J Dernores

Catfish (Clarias batrachus) serum lectin recognizes polyvalent Tn [alpha-D-GalpNAc1-Ser/Thr], Talpha [beta-D-Galp-(1-->3)-alpha-D-GalpNAc1-Ser/Thr], and II [beta-D-Galp(1-->4)-beta-D-GlcpNAc1-] mammalian glycotopes.

PubMed

Singha, Biswajit; Adhya, Mausumi; Chatterjee, Bishnu P

2008-09-22

A new calcium dependent GalNAc/Gal specific lectin was isolated from the serum of Indian catfish, Clarias batrachus and designated as C. batrachus lectin (CBL). It is a disulfide-linked homodecameric lectin of 74.65kDa subunits and the oligomeric form is essential for its activity. Binding specificity of CBL was investigated by enzyme-linked lectin-sorbent assay using a series of simple sugars, polysaccharides, and glycoproteins. GalNAc was more potent inhibitor than Gal; and alpha glycosides of both were more inhibitory than their beta counterparts. CBL showed maximum affinity for human tumor-associated Tn-antigens (GalNAcalpha1-Ser/Thr) at the molecular level and was 3.5 times higher than GalNAc. CBL interacted strongly with polyvalent Tn and Talpha (Galbeta1,3GalNAcalpha1-) as well as multivalent-II (Galbeta1,4GlcNAcbeta1-) antigens containing glycoproteins and intensity of inhibition was 10(3)-10(5) times more than monovalent ones. The overall specificity of CBL lies in the order of polyvalent Tn, Talpha and II>monovalent Tn > or = Me-alphaGalNAc>monovalent Talpha> Me-betaGalNAc>Me-alphaGal>monovalent T>GalNAc>monovalent F>monovalent II>Me-betaGal>Gal.

Estimation of sediment sources using selected chemical tracers in the Perry lake basin, Kansas, USA

USGS Publications Warehouse

Juracek, K.E.; Ziegler, A.C.

2009-01-01

The ability to achieve meaningful decreases in sediment loads to reservoirs requires a determination of the relative importance of sediment sources within the contributing basins. In an investigation of sources of fine-grained sediment (clay and silt) within the Perry Lake Basin in northeast Kansas, representative samples of channel-bank sources, surface-soil sources (cropland and grassland), and reservoir bottom sediment were collected, chemically analyzed, and compared. The samples were sieved to isolate the <63 ?? m fraction and analyzed for selected nutrients (total nitrogen and total phosphorus), organic and total carbon, 25 trace elements, and the radionuclide cesium-137 (137Cs). On the basis of substantial and consistent compositional differences among the source types, total nitrogen (TN), total phosphorus (TP), total organic carbon (TOC), and 137Cs were selected for use in the estimation of sediment sources. To further account for differences in particle-size composition between the sources and the reservoir bottom sediment, constituent ratio and clay-normalization techniques were used. Computed ratios included TOC to TN, TOC to TP, and TN to TP. Constituent concentrations (TN, TP, TOC) and activities (137Cs) were normalized by dividing by the percentage of clay. Thus, the sediment-source estimations involved the use of seven sediment-source indicators. Within the Perry Lake Basin, the consensus of the seven indicators was that both channel-bank and surface-soil sources were important in the Atchison County Lake and Banner Creek Reservoir subbasins, whereas channel-bank sources were dominant in the Mission Lake subbasin. On the sole basis of 137Cs activity, surface-soil sources contributed the most fine-grained sediment to Atchison County Lake, and channel-bank sources contributed the most fine-grained sediment to Banner Creek Reservoir and Mission Lake. Both the seven-indicator consensus and 137Cs indicated that channel-bank sources were dominant for

Integration of GCAM-USA into GLIMPSE: Update and ...

EPA Pesticide Factsheets

The purpose of this presentation is to (i) discuss changes made to the GCAM-USA model to more fully support long-term, coordinated environmental-climate-energy planning within the U.S., and (ii) demonstrate the graphical user interface that has been constructed to construct modeling scenarios, execute GCAM-USA, and visualize and compare model outputs. GLIMPSE is intended to provide insights into linkages and synergies among the goals of air quality management, climate change mitigation, and long-range energy planning. We have expanded GLIMPSE to also incorporate the open-source Global Change Assessment Model-USA (GCAM-USA), which has state-level representation of the U.S. energy system. With GCAM-USA, GLIMPSE can consider more aspects of the economy, linkages to the water and climate systems, and interactions with other regions of the world. A user-friendly graphical interface allows the system to be applied by analysts to explore a range of policies, such emission taxes or caps, efficiency standards, and renewable portfolio standards. We expect GLIMPSE to be used within research and planning activities, both within the EPA and beyond.

Echinococcus granulosus in gray wolves and ungulates in Idaho and Montana, USA.

PubMed

Foreyt, William J; Drew, Mark L; Atkinson, Mark; McCauley, Deborah

2009-10-01

We evaluated the small intestines of 123 gray wolves (Canis lupus) that were collected from Idaho, USA (n=63), and Montana, USA (n=60), between 2006 and 2008 for the tapeworm Echinococcus granulosus. The tapeworm was detected in 39 of 63 wolves (62%) in Idaho, USA, and 38 of 60 wolves (63%) in Montana, USA. The detection of thousands of tapeworms per wolf was a common finding. In Idaho, USA, hydatid cysts, the intermediate form of E. granulosus, were detected in elk (Cervus elaphus), mule deer (Odocoileus hemionus), and a mountain goat (Oreamnos americanus). In Montana, USA, hydatid cysts were detected in elk. To our knowledge, this is the first report of adult E. granulosus in Idaho, USA, or Montana, USA. It is unknown whether the parasite was introduced into Idaho, USA, and southwestern Montana, USA, with the importation of wolves from Alberta, Canada, or British Columbia, Canada, into Yellowstone National Park, Wyoming, USA, and central Idaho, USA, in 1995 and 1996, or whether the parasite has always been present in other carnivore hosts, and wolves became a new definitive host. Based on our results, the parasite is now well established in wolves in these states and is documented in elk, mule deer, and a mountain goat as intermediate hosts.

Magma plumbing in the Grímsvötn volcanic system, Iceland: an overview

NASA Astrophysics Data System (ADS)

Thordarson, T.

2016-12-01

The basaltic Grímsvötn volcanic system (GVS) consists of Grímsvötn central volcano (GCV) and an immature fissure swarm extending 70 km to the southwest from GCV. The GCV has the highest eruption frequency of all central volcanos in Iceland, or 7 events per 100 years. In contrast, the GVS fissure swarm has only featured two events in postglacial times, the 1783-4 Laki and the prehistoric Lambavatnsgígar fissure eruptions. These two events account for 25% of the total Holocene magma output from the GVS and 80% of the output in historic time (i.e. last 1100 years). Although GVS magma plumbing has been a topic of research for four decades, its general structure, extent and geometry is still deliberated. Is mantle-derived magma delivered straight up beneath the GCV to an upper crustal magma chamber and then vertically to eruptions at the GCV and laterally to eruption on the GVS fissure swarm? Or does the system feature two levels of crustal storage, one in the upper crust beneath GCV and another at mid-crustal depth? Or is the structure of the GVS plumbing more complex? The data that we have so far and is pertinent to GVS magma plumbing is summarised below: Geophysical measurements imply that shallowest magma storage beneath GCV is at 3-4 km. The Zr and Nb concentrations in the tephra from the 1998 and 2004 GCV plus Laki eruptions show that the parent magmas for each was produced by different degrees of partial melting of a similar mantle source. It also demonstrates transport to the surface via separate pathways and that neither magma can be derived by fractional crystallization from a Laki-like magma. Detailed petrological studies on the Laki tephra and lava indicate polybaric magma evolution within the mid-crust (at 6 to 15 km depth), with further evolution at shallower depths induced either by disequilibrium crystal growth during ascent of magma from the mid-crust storage or a brief residence at 3-6 km depths. The Laki magma contains significant abundances of

Comparison of windthrow damage among thinned and unthinned hardwoods and adjacent pine stands following a late winter tornado on the Cumberland Plateau near Sewanee, TN

Treesearch

Karen Kuers; Samuel C. Grinstead

2006-01-01

In March, 2004, prior to spring leaf-out, an F0 tornado snapped or uprooted 672 trees across 25 ha of the western margin of the Cumberland Plateau near Sewanee, TN. A ridgeline separated the impacted area into two parts: westward-facing slopes of mature hardwoods and eastward-facing slopes with thinned and unthinned hardwoods, pine stands, and clearcuts. Greatest...

"USA Today": Can the Nation's Newspaper Survive?

ERIC Educational Resources Information Center

Wicks, Robert H.

The failure of 17 newspaper markets between 1957 and 1975 raises the question of whether the 1982 entrance of "USA Today" into the newspaper market demonstrated fiscal prudence. A 20-month advertising content analysis was conducted to assess advertising trends in "USA Today." These data were compared with industry statistics…

77 FR 47671 - TA-W-81,520, T-Mobile USA, Inc., Call Center, Allentown, PA; TA-W-81,520G, T-Mobile USA, Inc...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-08-09

... DEPARTMENT OF LABOR Employment and Training Administration TA-W-81,520, T-Mobile USA, Inc., Call Center, Allentown, PA; TA- W-81,520G, T-Mobile USA, Inc., Headquarters Office, Bellevue, WA; Amended... of T-Mobile USA, Inc., Call Center, Allentown, Pennsylvania (TA-W-81,520), Fort Lauderdale, Florida...

Evolving plans for the USA National Phenology Network

USGS Publications Warehouse

Betancourt, Julio L.; Schwartz, Mark D.; Breshears, David D.; Brewer, Carol A.; Frazer, Gary; Gross, John E.; Mazer, Susan J.; Reed, Bradley C.; Wilson, Bruce E.

2007-01-01

Phenology is the study of periodic plant and animal life cycle events, how these are influenced by seasonal and interannual variations in climate, and how they modulate the abundance, diversity, and interactions of organisms. The USA National Phenology Network (USA-NPN) is currently being organized to engage federal agencies, environmental networks and field stations, educational institutions, and citizen scientists. The first USA-NPN planning workshop was held August 2005, in Tucson, Ariz. (Betancourt et al. [2005]; http://www.uwm.edu/Dept/Geography/npn/; by 1 June 2007, also see http://www.usanpn.org). With sponsorship from the U.S. National Science Foundation, the U.S. Geological Survey (USGS), the U.S. Fish and Wildlife Service, and NASA, the second USA-NPN planning workshop was held at the University of Wisconsin-Milwaukee on 10–12 October 2006 to (1) develop lists of target species and observation protocols; (2) identify existing networks that could comprise the backbone of nationwide observations by 2008; (3) develop opportunities for education, citizen science, and outreach beginning in spring 2007; (4) design strategies for implementing the remote sensing component of USA-NPN; and (5) draft a data management and cyberinfrastructure plan.

The Sixth U.S.A. Mathematical Olympiad

ERIC Educational Resources Information Center

Greitzer, Samuel L.

1978-01-01

One hundred and seven students of 365,000 students given the annual High School Mathematics Examination took part in the sixth U.S.A. Mathematical Olympiad. The top eight scores are listed along with comparative Exam and Olympiad scores for the group and individual problem scores. The problems proposed on the U.S.A. Olympiad on an International…

Flynn Creek Impact Structure: New Insights from Breccias, Melt Features, Shatter Cones, and Remote Sensing

NASA Technical Reports Server (NTRS)

Evenick, J. C.; Lee, P.; Deane, B.

2004-01-01

The Flynn Creek impact structure is located in Tennessee, USA (36 deg.17 min.N, 85 deg.40 min.W). The structure was first mapped as a crypto-volcanic by Wilson and Born in 1936 [1]. Although they did not properly identify the stratigraphy within the crater or the causal mechanism, they did correctly define the horizontal extent of the crater. More detailed surface and subsurface research by Roddy (1979) accurately described the crater as being an impact structure with a diameter of 3.8 km. It formed around 360 Ma, which corresponds to the interval between the deposition of the Nashville Group and the Chattanooga Shale. Although there is limited rock outcrop in the area, there are exposed surface faults, folds, and large outcrops of impact breccia within the crater.

Balanced crystalloids versus saline in the intensive care unit: study protocol for a cluster-randomized, multiple-crossover trial.

PubMed

Semler, Matthew W; Self, Wesley H; Wang, Li; Byrne, Daniel W; Wanderer, Jonathan P; Ehrenfeld, Jesse M; Stollings, Joanna L; Kumar, Avinash B; Hernandez, Antonio; Guillamondegui, Oscar D; May, Addison K; Siew, Edward D; Shaw, Andrew D; Bernard, Gordon R; Rice, Todd W

2017-03-16

Saline, the intravenous fluid most commonly administered to critically ill adults, contains a high chloride content, which may be associated with acute kidney injury and death. Whether using balanced crystalloids rather than saline decreases the risk of acute kidney injury and death among critically ill adults remains unknown. The Isotonic Solutions and Major Adverse Renal Events Trial (SMART) is a pragmatic, cluster-level allocation, cluster-level crossover trial being conducted between 1 June 2015 and 30 April 2017 in five intensive care units at Vanderbilt University Medical Center in Nashville, TN, USA. SMART compares saline (0.9% sodium chloride) with balanced crystalloids (clinician's choice of lactated Ringer's solution or Plasma-Lyte A®). Each intensive care unit is assigned to provide either saline or balanced crystalloids each month, with the assigned crystalloid alternating monthly over the course of the trial. All adults admitted to participating intensive care units during the study period are enrolled and followed until hospital discharge or 30 days after enrollment. The anticipated enrollment is approximately 14,000 patients. The primary outcome is Major Adverse Kidney Events within 30 days-the composite of in-hospital death, receipt of new renal replacement therapy, or persistent renal dysfunction (discharge creatinine ≥200% of baseline creatinine). Secondary clinical outcomes include in-hospital mortality, intensive care unit-free days, ventilator-free days, vasopressor-free days, and renal replacement therapy-free days. Secondary renal outcomes include new renal replacement therapy receipt, persistent renal dysfunction, and incidence of stage 2 or higher acute kidney injury. This ongoing pragmatic trial will provide the largest and most comprehensive comparison to date of clinical outcomes with saline versus balanced crystalloids among critically ill adults. For logistical reasons, SMART was prospectively registered separately for the medical

First detection of the staphylococcal trimethoprim resistance gene dfrK and the dfrK-carrying transposon Tn559 in enterococci.

PubMed

López, María; Kadlec, Kristina; Schwarz, Stefan; Torres, Carmen

2012-02-01

The trimethoprim resistance gene dfrK has been recently described in Staphylococcus aureus, but so far has not been found in other bacteria. A total of 166 enterococci of different species (E. faecium, E. faecalis, E. hirae, E. durans, E. gallinarum, and E. casseliflavus) and origins (food, clinical diseases in humans, healthy humans or animals, and sewage) were studied for their susceptibility to trimethoprim as determined by agar dilution (European Committee on Antimicrobial Susceptibility Testing) and the presence of (a) the dfrK gene and its genetic environment and (b) other dfr genes. The dfrK gene was detected in 49% of the enterococci (64% and 42% of isolates with minimum inhibitory concentrations of ≥2 mg/L or ≤1 mg/L, respectively). The tet(L)-dfrK linkage was detected in 21% of dfrK-positive enterococci. The chromosomal location of the dfrK gene was identified in one E. faecium isolate in which the dfrK was not linked to tet(L) gene but was part of a Tn559 element, which was integrated in the chromosomal radC gene. This Tn559 element was also found in 14 additional isolates. All combinations of dfr genes were detected among the isolates tested (dfrK, dfrG, dfrF, dfrK+dfrG, dfrK+dfrF, dfrF+dfrG, and dfrF+dfrG+dfrK). The gene dfrK gene was found together with other dfr genes in 58% of the tested enterococci. This study suggested an exchange of the trimethoprim resistance gene dfrK between enterococci and staphylococci, as previously observed for the trimethoprim resistance gene dfrG.

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

Attendees of the USA Science and Engineering Festival observe their infrared images as a NASA Staff member describes the James Webb Space Telescope. It will be a large infrared telescope with a 6.5 meter primary mirror and will study every phase in the history of our Universe ranging from the Big Bang to the formation of our Solar System. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

Effects of dexmedetomidine on H-FABP, CK-MB, cTnI levels, neurological function and near-term prognosis in patients undergoing heart valve replacement.

PubMed

Wang, Zhi; Chen, Qiang; Guo, Hao; Li, Zhishan; Zhang, Jinfeng; Lv, Lei; Guo, Yongqing

2017-12-01

This study investigated the effects of dexmedetomidine on heart-type fatty acid binding protein (H-FABP), creatine kinase isoenzymes (CK-MB), and troponin I (cTnI) levels, neurological function and near-term prognosis in patients undergoing heart valve replacement. Patients undergoing heart valve replacement were randomly allocated to remifentanil anesthesia (control group, n=48) or dexmedetomidine anesthesia (observation group, n=48). Hemodynamic parameters were measured before anesthesia induction (T1), 1 min after intubation (T2), 10 min after start of surgery (T3), and on completion of surgery (T4). Levels of plasma H-FABP, CK-MB and cTnI were measured 10 min before anesthesia induction (C1), 10 min after start of surgery (C2), on completion of surgery (C3), 6 h after surgery (C4), and 24 h after surgery (C5). S100β protein and serum neuron-specific enolase (NSE) were detected 10 min before anesthesia induction (C1), and 24 h after surgery (C5). Neurological and cardiac function was evaluated 24 h after surgery. Incidence of cardiovascular adverse events was recorded for 1 year of follow-up. There were no significant differences in the average heart rate between the two groups during the perioperative period. The mean arterial pressure in the observation group was significantly lower than control group (P<0.05). Levels of H-FABP, CK-MB and cTnI at C2, C3, C4 and C5, were significantly higher than C1, but significantly lower in the observation versus control group (P<0.05). Twenty-four hours after surgery, levels of S100β and NSE in both groups were higher than those before induction (P<0.05), but significantly lower in the observation versus control group (P<0.05). Twenty-four hours after surgery, neurological function scores were better, and myocardial contractility and arrhythmia scores significantly lower in the observation versus control group (P<0.05 for all). After follow-up for 1 year, incidence of cardiovascular adverse events was significantly lower

Airborne surveys of USA urban areas at 121.5/243 MHz

NASA Technical Reports Server (NTRS)

Taylor, R. E.; Hill, J. S.

1979-01-01

In situ, aircraft flight measurements were made in 1976 and 1977 by NASA of the radio-frequency environment over USA urban areas within the emergency distress search and rescue frequency bands at 121.5 and 243.0 MHz. This paper analyzes test results reported previously for USA East Coast and Midwest flight surveys; presented also are test results obtained in May 1977 for the USA West Coast during the NASA, ASSESS-II, Space Shuttle/Spacelab simulation aircraft flights. The USA West Coast flight include data at 121.5/243 MHz during an extensive series of aircraft passes for the Los Angeles urban area. The USA East Coast/Midwest measurements show correlation with population count.

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

An attendee of the USA Science and Engineering Festival observes the infrared image of himself as a NASA staff member describes the James Webb Space Telescope. It will be a large infrared telescope with a 6.5 meter primary mirror and will study every phase in the history of our Universe ranging from the Big Bang to the formation of our Solar System. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

An attendee of the USA Science and Engineering Festival examines how glass blocks some heat, altering the infrared image of himself. The James Webb Space Telescope will be a large infrared telescope with a 6.5 meter primary mirror and will study every phase in the history of our Universe ranging from the Big Bang to the formation of our Solar System. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

An attendee of the USA Science and Engineering Festival observes the infrared image of himself as a NASA Staff member describes the James Webb Space Telescope. It will be a large infrared telescope with a 6.5 meter primary mirror and will study every phase in the history of our Universe ranging from the Big Bang to the formation of our Solar System. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

An attendee of the USA Science and Engineering Festival observes Robonaut 2 at the NASA Stage. Robonaut 2 is NASA's first dexterous humanoid robot that has been working on the International Space Station for the last three years. R2 recently received 1.2 meter long legs to allow mobility. This will enable R2 to assist more with regular and repetitive tasks inside and outside the station. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

Investigating habitat value to inform contaminant remediation options: case study

Treesearch

Rebecca A. Efroymson; Mark J. Peterson; Neil R. Giffen; Michael G. Ryon; John G. Smith; William W. Hargrove; W. Kelly Roy; Christopher J. Welsh; Daniel L. Druckenbrod; Harry D. Quarles

2008-01-01

Habitat valuation methods were implemented to support remedial decisions for aquatic and terrestrial contaminated sites at the East Tennessee Technology Park (ETTP) on the US Department of Energy (DOE) Oak Ridge Reservation in Oak Ridge, TN, USA. The habitat valuation was undertaken for six contaminated sites: Contractor’s Spoil Area, K-901-N Disposal Area, K-770...

Carbon stable isotope (δ13C) and elemental (TOC, TN, C/N) geochemistry in salt marsh surface sediments (Western Brittany, France): Adequate proxies for relative sea-level reconstruction?

NASA Astrophysics Data System (ADS)

Goslin, Jerome; Sans-jofre, Pierre; Van Vliet Lanoë, Brigitte; Delacourt, Christophe

2017-04-01

Reconstructing a dense network of precise and reliable records of Holocene relative sea-level (RSL) changes is still a major challenge for the paleo climate scientific community. In some regions, the use of traditional foraminifera-based transfer function is prevented by micro-fauna scarcity (e.g. Stéphan et al., 2014, Goslin et al., 2015), thus fostering the need for alternative proxies to be developed and used. Rather recently, isotopic and elemental geochemistry tools have been shown to form promising alternative proxies for RSL reconstruction (e.g. Wilson et al., 2005, Engelhart et al., 2013, Khan et al., 2015). Questions remain nonetheless open regarding the possibility for such markers to allow (i) distinguishing between freshwater and brackish to marine domains (this condition being needed if RSL index-points are to be derived from sedimentary markers) and (ii) to adequately identify the source of the organic matter preserved in the sediment. Concerns about the preservation of carbon and nitrogen compounds during diagenesis have also arose questioning the reliability of such markers for paleo-environmental reconstruction purposes (Wilson et al., 2005; Lamb et al., 2006). We analyzed stable carbon isotope ratios (δ13C), Total Organic Carbon (TOC), and Total Nitrogen (TN) values within 94 surface sediments sampled across two C-3 plants dominated saltmarshes (Brittany, France). The distributions of δ13C, TOC, TN and C/N values is observed to follow clear and strong elevation-dependent trends. Some slight local variability appears between the studied sites that can be easily explained by the different morphological configuration and functioning of these latter. An indicator is found that allows sediments from below and above the high-tide level to be discriminated. This finding forms an interesting advance in the field as it permits to ensure that samples formed under saline conditions and thus suggests that these can be used as stand-alone proxies for RSL

Comparative Review of UK-USA Industry-University Relationships

ERIC Educational Resources Information Center

Decter, Moira H.

2009-01-01

Purpose: The purpose of this paper is to explore significant historical changes, legislation and policy in the UK and USA from the 1960s to present day relating to university-industry relationships. Design/methodology/approach: The paper presents a review of papers, reports and policy documents from the UK and USA drawing comparisons of…

Why Does the USA Dominate University League Tables?

ERIC Educational Resources Information Center

Li, Mei; Shankar, Sriram; Tang, Kam Ki

2011-01-01

According to the Academic Ranking of World Universities, the world's top 500 universities are located in only 38 countries, with the USA alone having 157 of them. This article investigates the socioeconomic determinants of the wide performance gap between countries, and whether the USA's dominance is largely due to its economic power. A large…

Pricing behavior of USA exporter in wheat international market

NASA Astrophysics Data System (ADS)

Wibowo, R. P.; Sumono; Iddrisu, Y.; Darus, M.; Sihombing, L. P.; Jufri

2018-02-01

The number of wheat producing countries is changing over time. It is expected the change in wheat supply will lead world wheat market become more competitive and reduce market power of major exporter country. This paper tries to identify and examined the degree of market power on wheat international market for USA by using the Pricing to Market (PTM) method. USA is the biggest producer and exporter in wheat market. The PTM method found that USA impose noncompetitive strategy by applying price discrimination and apply market power to their importer country.

Diagnostic value of 3D time-of-flight MRA in trigeminal neuralgia.

PubMed

Cai, Jing; Xin, Zhen-Xue; Zhang, Yu-Qiang; Sun, Jie; Lu, Ji-Liang; Xie, Feng

2015-08-01

The aim of this meta-analysis was to evaluate the diagnostic value of 3D time-of-flight magnetic resonance angiography (3D-TOF-MRA) in trigeminal neuralgia (TN). Relevant studies were identified by computerized database searches supplemented by manual search strategies. The studies were included in accordance with stringent inclusion and exclusion criteria. Following a multistep screening process, high quality studies related to the diagnostic value of 3D-TOF-MRA in TN were selected for meta-analysis. Statistical analyses were conducted using Statistical Analysis Software (version 8.2; SAS Institute, Cary, NC, USA) and Meta Disc (version 1.4; Unit of Clinical Biostatistics, Ramon y Cajal Hospital, Madrid, Spain). For the present meta-analysis, we initially retrieved 95 studies from database searches. A total of 13 studies were eventually enrolled containing a combined total of 1084 TN patients. The meta-analysis results demonstrated that the sensitivity and specificity of the diagnostic value of 3D-TOF-MRA in TN were 95% (95% confidence interval [CI] 0.93-0.96) and 77% (95% CI 0.66-0.86), respectively. The pooled positive likelihood ratio and negative likelihood ratio were 2.72 (95% CI 1.81-4.09) and 0.08 (95% CI 0.06-0.12), respectively. The pooled diagnostic odds ratio of 3D-TOF-MRA in TN was 52.92 (95% CI 26.39-106.11), and the corresponding area under the curve in the summary receiver operating characteristic curve based on the 3D-TOF-MRA diagnostic image of observers was 0.9695 (standard error 0.0165). Our results suggest that 3D-TOF-MRA has excellent sensitivity and specificity as a diagnostic tool for TN, and that it can accurately identify neurovascular compression in TN patients. Copyright © 2015 Elsevier Ltd. All rights reserved.

Basque Diaspora in the USA and Language Maintenance

ERIC Educational Resources Information Center

Lasagabaster, David

2008-01-01

The Basques first immigrated on a large scale to the USA during the Gold Rush of 1848. After immigrating to the USA, they settled in pockets throughout the West, especially in California, Nevada and Idaho, and it is currently estimated that more than 35,000 Basque-Americans live in these three states. This represents one of the largest…

Perceived discrimination among three groups of refugees resettled in the USA: associations with language, time in the USA, and continent of origin.

PubMed

Hadley, Craig; Patil, Crystal

2009-12-01

The objectives of this study were to assess the prevalence and predictors of discrimination among a community-based sample of refugees resettled in the USA. We sought to test whether language, gender, time in the USA and country of origin were associated with the experience of discrimination among individuals resettled in the USA as part of the refugee resettlement program. Perceived discrimination was assessed among individuals from East Africa (n = 92), West Africa (n = 74), and from Eastern Europe (n = 112) using a multi-item measure of discrimination. Bivariate associations revealed statistically significant associations between experiences of discrimination and time in the USA, language ability, and sending country. A logistic regression model revealed that refugees from African sending countries were more likely than Eastern European individuals to experience discrimination, even after controlling for potentially confounding factors. We interpret this finding as evidence of racism and discuss the implications for population health and resettlement practice.

Triboelectric charging of volcanic ash from the 2011 Grímsvötn eruption.

PubMed

Houghton, Isobel M P; Aplin, Karen L; Nicoll, Keri A

2013-09-13

The plume from the 2011 eruption of Grímsvötn was highly electrically charged, as shown by the considerable lightning activity measured by the United Kingdom Met Office's low-frequency lightning detection network. Previous measurements of volcanic plumes have shown that ash particles are electrically charged up to hundreds of kilometers away from the vent, which indicates that the ash continues to charge in the plume [R. G. Harrison, K. A. Nicoll, Z. Ulanowski, and T. A. Mather, Environ. Res. Lett. 5, 024004 (2010); H. Hatakeyama J. Meteorol. Soc. Jpn. 27, 372 (1949)]. In this Letter, we study triboelectric charging of different size fractions of a sample of volcanic ash experimentally. Consistently with previous work, we find that the particle size distribution is a determining factor in the charging. Specifically, our laboratory experiments demonstrate that the normalized span of the particle size distribution plays an important role in the magnitude of charging generated. The influence of the normalized span on plume charging suggests that all ash plumes are likely to be charged, with implications for remote sensing and plume lifetime through scavenging effects.

Triboelectric Charging of Volcanic Ash from the 2011 Grímsvötn Eruption

NASA Astrophysics Data System (ADS)

Houghton, Isobel M. P.; Aplin, Karen L.; Nicoll, Keri A.

2013-09-01

The plume from the 2011 eruption of Grímsvötn was highly electrically charged, as shown by the considerable lightning activity measured by the United Kingdom Met Office’s low-frequency lightning detection network. Previous measurements of volcanic plumes have shown that ash particles are electrically charged up to hundreds of kilometers away from the vent, which indicates that the ash continues to charge in the plume [R. G. Harrison, K. A. Nicoll, Z. Ulanowski, and T. A. Mather, Environ. Res. Lett. 5, 024004 (2010)1748-932610.1088/1748-9326/5/2/024004; H. Hatakeyama J. Meteorol. Soc. Jpn. 27, 372 (1949)JMSJAU0026-1165]. In this Letter, we study triboelectric charging of different size fractions of a sample of volcanic ash experimentally. Consistently with previous work, we find that the particle size distribution is a determining factor in the charging. Specifically, our laboratory experiments demonstrate that the normalized span of the particle size distribution plays an important role in the magnitude of charging generated. The influence of the normalized span on plume charging suggests that all ash plumes are likely to be charged, with implications for remote sensing and plume lifetime through scavenging effects.

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

An attendee of the USA Science and Engineering Festival is measured by a laser at the NASA Stage. A NASA Staff member describes the Ice, Cloud, and land Elevation Satellite (ICESat) mission, which operated from 2003-2009, and pioneered the use of laser altimeters in space to study the elevation of the Earth's surface and its changes. ICESat-2 is a follow-on mission to continue the ICESat observations and is scheduled to launch in 2017. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

Two boys attending the USA Science and Engineering Festival pose with Robonaut 2 at the NASA Stage. Robonaut 2 is NASA's first dexterous humanoid robot that has been working on the International Space Station for the last three years. R2 recently received 1.2 meter long legs to allow mobility. This will enable R2 to assist more with regular and repetitive tasks inside and outside the station. The USA Science and Engineering Festival took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

Insecticide Exposure in Parkinsonism

DTIC Science & Technology

2006-01-01

Chlorpyrifos or Permethrin Exposures, fall 2000, National Meeting of the Society for Environmental Toxicology and Chemistry , Nashville, Tennessee. D. Karen...Biomarkers, fall 2000, National Meeting of the Society for Environmental Toxicology and Chemistry , Nashville, Tennessee. D. Karen, W. Li, P. Harp, J...by the U.S. Army, contract DAMD-17-98-1-8633. Abstracts for Society of Environmental Toxicology and Chemistry meeting, Fall, 2000: Murine

Capability Description for NASA's F/A-18 TN 853 as a Testbed for the Integrated Resilient Aircraft Control Project

NASA Technical Reports Server (NTRS)

Hanson, Curt

2009-01-01

The NASA F/A-18 tail number (TN) 853 full-scale Integrated Resilient Aircraft Control (IRAC) testbed has been designed with a full array of capabilities in support of the Aviation Safety Program. Highlights of the system's capabilities include: 1) a quad-redundant research flight control system for safely interfacing controls experiments to the aircraft's control surfaces; 2) a dual-redundant airborne research test system for hosting multi-disciplinary state-of-the-art adaptive control experiments; 3) a robust reversionary configuration for recovery from unusual attitudes and configurations; 4) significant research instrumentation, particularly in the area of static loads; 5) extensive facilities for experiment simulation, data logging, real-time monitoring and post-flight analysis capabilities; and 6) significant growth capability in terms of interfaces and processing power.

Comparison of Cancer Incidence between China and the USA.

PubMed

Wang, Yong-Chuan; Wei, Li-Juan; Liu, Jun-Tian; Li, Shi-Xia; Wang, Qing-Sheng

2012-06-01

The incidence of cancer varies around the globe, especially between less-developed and developed regions. The aim of this study is to explore differences in cancer incidence between China and the USA. Data were obtained from the GLOBOCAN 2008 database. Estimated numbers of new cancer cases in the USA were obtained from the American Cancer Society, while the numbers of cases in China, including those in urban and rural areas, were obtained from 36 cancer registries (2003-2005). Cancer incidence for major sites between China and the USA were analyzed. In China, lung cancer was the predominant type of cancer detected in males; in females, breast cancer was the main type of cancer. Gastrointestinal cancers, such as those of the liver, stomach, and esophagus, were more commonly seen in China than in the USA. A significant difference in the incidence of melanoma of the skin was observed between China and the USA. During comparison of differences in the age-standardized rates by world population (ASRWs) of major cancer sites between the two countries, 4 sites in males (i.e., nasopharynx, esophagus, stomach, and liver) and 6 sites in females (i.e., nasopharynx, esophagus, stomach, liver, gallbladder, and cervix uteri) showed higher cancer incidence rates in China than in the USA. Significant differences in cancer incidence sites were found between the two countries. Cancer may be prevented through public education and awareness. Programs to promote cancer prevention in China, especially those of the lung, breast, and gastrointestinal region, must also be implemented.

78 FR 14400 - Southern USA Resources, Inc., Order of Suspension of Trading

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-05

... SECURITIES AND EXCHANGE COMMISSION [File No. 500-1] Southern USA Resources, Inc., Order of... lack of current and accurate information concerning the securities of Southern USA Resources, Inc. (``Southern USA'') because of questions regarding the accuracy of publicly-disseminated information concerning...

History of Primary Magnesium Since World War II

NASA Astrophysics Data System (ADS)

Clow, Byron B.

Primary magnesium production just prior to WWII was around 32,000 tonnes with most of it coming from Germany and England with smaller amounts from France and the U.S.A. Peak production during the war was 232,000 tonnes with most of it coming from the 15 plants that had been built in the United States. By 1946 worldwide demand had fallen back to pre-war levels until 1980. The paper will identify the various plants built round the world since WWII and the impact of exports from Russia and China beginning in the early 90's. "The presentation will be by country in chronological order. We will not discuss the 15 or 20 potential projects going on around the world since this was well covered by Bob Brown at the Nashville Conference in the year 2000.

The USA PATRIOT Act.

ERIC Educational Resources Information Center

Minow, Mary; Coyle, Karen; Kaufman, Paula

2002-01-01

Explains the USA PATRIOT (Uniting and Strengthening America by Providing Appropriate Tools Required to Intercept and Obstruct Terrorism) Act, passed after the September 11 terrorist attacks, and its implications for libraries and patron records. Considers past dealings with the FBI; court orders; search warrants; wiretaps; and subpoenas. Includes:…

OBIS-USA: a data-sharing legacy of census of marine life

USGS Publications Warehouse

Sedberry, George R.; Fautin, Daphne G.; Feldman, Michael; Fornwall, Mark D.; Goldstein, Phillip; Guralnick, Robert P.

2011-01-01

The United States Geological Survey's Biological Informatics Program hosts OBIS-USA, the US node of the Ocean Biogeographic Information System (OBIS). OBIS-USA gathers, coordinates, applies standard formats to, and makes widely available data on biological collections in marine waters of the United States and other areas where US investigators have collected data and, in some instances, specimens. OBIS-USA delivers its data to OBIS international, which then delivers its data to the Global Biodiversity Information Facility (GBIF) and other Web portals for marine biodiversity data. OBIS-USA currently has 145 data sets from 36 participants, representing over 6.5 million occurrence records of over 83,000 taxa from more than 888,000 locations. OBIS-USA, a legacy of the decade-long (2001–2010) international collaborative Census of Marine Life enterprise, continues to add data, including those from ongoing Census projects. Among the many challenges in creating OBIS, including OBIS-USA, were developing a community of trust and shared value among data providers, and demonstrating to providers the value of making their data accessible to others. Challenges also posed by the diversity of data sets relevant to marine biodiversity stored on thousands of computers, in a variety of formats, not all widely accessible, have been met in OBIS-USA by implementing a uniform standard and publishing platform that is easily accessible to a broad range of users.

Chlorhexidine gluconate reduces transmission of methicillin-resistant Staphylococcus aureus USA300 among Marine recruits.

PubMed

Whitman, Timothy J; Schlett, Carey D; Grandits, Greg A; Millar, Eugene V; Mende, Katrin; Hospenthal, Duane R; Murray, Patrick R; Tribble, David R

2012-08-01

Methicillin-resistant Staphylococcus aureus (MRSA) pulsed-field type (PFT) USA300 causes skin and soft tissue infections in military recruits and invasive disease in hospitals. Chlorhexidine gluconate (CHG) is used to reduce MRSA colonization and infection. The impact of CHG on the molecular epidemiology of MRSA is not known. To evaluate the impact of 2% CHG-impregnated cloths on the molecular epidemiology of MRSA colonization. Cluster-randomized, double-blind, controlled trial. Marine Officer Candidate School, Quantico, Virginia, in 2007. Military recruits. Thrice-weekly application of CHG-impregnated or control (Comfort Bath; Sage) cloths over the entire body. Baseline and serial (every 2 weeks) nasal and/or axillary swab samples were assessed for MRSA colonization. Molecular analysis was performed with pulsed-field gel electrophoresis. During training, 77 subjects (4.9%) acquired MRSA, 26 (3.3%) in the CHG group and 51 (6.5%) in the control group (P=.004). When analyzed for PFT, 24 subjects (3.1%) in the control group but only 6 subjects (0.8%) in the CHG group (P=.001) had USA300. Of the 167 colonizing isolates recovered from 77 subjects, 99 were recovered from the control group, including USA300 (40.4%), USA800 (38.4%), USA1000 (12.1%), and USA100 (6.1%), and 68 were recovered from the CHG group, including USA800 (51.5%), USA100 (23.5%), and USA300 (13.2%). CHG decreased the transmission of MRSA--more specifically, USA300--among military recruits. In addition, USA300 and USA800 outcompeted other MRSA PFTs at incident colonization. Future studies should evaluate the broad-based use of CHG to decrease transmission of USA300 in hospital settings.

78 FR 1252 - Schneider Electric, U.S.A., Subsidiary of Schneider Electric, Power Business Unit, Power...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-08

... Workers From Volt Workforces Solutions and Resource Tek, Lavergne, TN; Amended Certification Regarding Eligibility To Apply for Worker Adjustment Assistance In accordance with Section 223 of the Trade Act of 1974... to Apply for Worker Adjustment Assistance on February 7, 2012, applicable to workers of Schneider...

Clinical outcomes of osteomyelitis patients infected with methicillin-resistant Staphylococcus aureus USA-300 strains.

PubMed

Peyrani, P; Allen, M; Seligson, D; Roberts, C; Chen, A; Haque, N; Zervos, M; Wiemken, T; Harting, J; Christensen, D; Ramirez, R

2012-03-01

Methicillin-resistant Staphylococcus aureus (MRSA) USA-300 strains have emerged as an important cause of community-acquired infections. These strains have been recognized as an etiology of osteomyelitis but data on their incidence and outcomes are limited. We retrospectively studied the incidence and clinical outcomes of MRSA USA-300 osteomyelitis in patients at the University of Louisville Hospital and the Henry Ford Health System between January 2007 and March 2008. Pulsed-field gel electrophoresis was used to determine USA type. Clinical outcomes were defined as management success versus failure at 12 months. Chi-square tests, Fisher exact tests, and Mann-Whitney tests were used to compare patient characteristics on the basis of clinical outcomes and USA type. Of the 50 patients with MRSA osteomyelitis, 27 (54%) had the USA-300 strain. Clinical failure was identified in 22% (6/27) of the patients with MRSA USA-300 and in 30% (7/23) of the patients with MRSA non-USA-300 osteomyelitis (P = .509). Our results showed that MRSA USA-300 is a significant etiology of MRSA osteomyelitis. With current surgical and medical management, outcomes of patients with MRSA USA-300 osteomyelitis are similar to those of patients with MRSA non-USA-300 osteomyelitis.

76 FR 29257 - Accreditation of Intertek USA, Inc., as a Commercial Laboratory

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-20

... DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection Accreditation of Intertek USA... Security. ACTION: Notice of accreditation of Intertek USA, Inc., as a commercial laboratory. SUMMARY: Notice is hereby given that, pursuant to 19 CFR 151.12, Intertek USA, Inc., 8500 West Bay Road MS 37...

Chlorhexidine Gluconate Reduces Transmission of Methicillin-Resistant Staphylococcus aureus USA300 among Marine Recruits

PubMed Central

Whitman, Timothy J.; Schlett, Carey D.; Grandits, Greg A.; Millar, Eugene V.; Mende, Katrin; Hospenthal, Duane R.; Murray, Patrick R.; Tribble, David R.

2018-01-01

BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) pulsed-field type (PFT) USA300 causes skin and soft tissue infections in military recruits and invasive disease in hospitals. Chlorhexidine gluconate (CHG) is used to reduce MRSA colonization and infection. The impact of CHG on the molecular epidemiology of MRSA is not known. OBJECTIVE To evaluate the impact of 2% CHG–impregnated cloths on the molecular epidemiology of MRSA colonization. DESIGN Cluster-randomized, double-blind, controlled trial. SETTING Marine Officer Candidate School, Quantico, Virginia, in 2007. PARTICIPANTS Military recruits. INTERVENTION Thrice-weekly application of CHG-impregnated or control (Comfort Bath; Sage) cloths over the entire body. MEASUREMENTS Baseline and serial (every 2 weeks) nasal and/or axillary swab samples were assessed for MRSA colonization. Molecular analysis was performed with pulsed-field gel electrophoresis. RESULTS During training, 77 subjects (4.9%) acquired MRSA, 26 (3.3%) in the CHG group and 51 (6.5%) in the control group (P = .004). When analyzed for PFT, 24 subjects (3.1%) in the control group but only 6 subjects (0.8%) in the CHG group (P = .001) had USA300. Of the 167 colonizing isolates recovered from 77 subjects, 99 were recovered from the control group, including USA300 (40.4%), USA800 (38.4%), USA1000 (12.1%), and USA100 (6.1%), and 68 were recovered from the CHG group, including USA800 (51.5%), USA100 (23.5%), and USA300 (13.2%). CONCLUSIONS CHG decreased the transmission of MRSA—more specifically, USA300—among military recruits. In addition, USA300 and USA800 outcompeted other MRSA PFTs at incident colonization. Future studies should evaluate the broad-based use of CHG to decrease transmission of USA300 in hospital settings. PMID:22759549

FY2015 Analysis of the Teamwork USA Program. Memorandum

ERIC Educational Resources Information Center

Howard, Mark

2015-01-01

The Department of Research and Evaluation (DRE) has completed an analysis of the performance of students who participated in the Teamwork USA Program, administered in FY2014 at three District schools. Teamwork USA hopes to improve student achievement at select Title I elementary schools via its Instrumental Music Program grant. This memorandum to…

75 FR 9592 - National Grid USA; Notice of Filing

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-03

... DEPARTMENT OF ENERGY Federal Energy Regulatory Commission [Docket No. AC10-50-000; RM08-5-000] National Grid USA; Notice of Filing February 24, 2010. Take notice that on February 10, 2010, National Grid USA submitted a request for waiver of certain FERC Form No. 1 reporting requirements under Part 141 of...

77 FR 30027 - Manufacturer of Controlled Substances; Notice of Registration; Siegfried (USA)

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-21

...; Notice of Registration; Siegfried (USA) By Notice dated January 6, 2012, and published in the Federal Register on January 17, 2012, 77 FR 2323, Siegfried (USA), 33 Industrial Park Road, Pennsville, New Jersey... U.S.C. 823(a) and determined that the registration of Siegfried (USA), to manufacture the listed...

77 FR 75183 - Approval of Intertek USA, Inc., as a Commercial Gauger

Federal Register 2010, 2011, 2012, 2013, 2014

2012-12-19

... DEPARTMENT OF HOMELAND SECURITY U.S. Customs and Border Protection Approval of Intertek USA, Inc.... ACTION: Notice of approval of Intertek USA, Inc., as a commercial gauger. SUMMARY: Notice is hereby given that, pursuant to 19 CFR 151.13, Intertek USA, Inc., 139 Castle Coakley, Suite 8, St. Croix, VI 00820...

Chicago, Illinois, USA

NASA Image and Video Library

1990-03-04

In this late winter scene of Chicago, Illinois, USA (41.5N, 87.0W) the light dusting of snow has actually enhanced the determination of the cities street pattern, parks and other cultural features. Sited at the south end of Lake Michigan, Chicago has long served as an industrial, transportation and communications center for the midwest. The obvious snowline on the ground enables meteorologists to trace the regional groundtracks of winter storms.

Novel anti-Sialyl-Tn monoclonal antibodies and antibody-drug conjugates demonstrate tumor specificity and anti-tumor activity.

PubMed

Prendergast, Jillian M; Galvao da Silva, Ana Paula; Eavarone, David A; Ghaderi, Darius; Zhang, Mai; Brady, Dane; Wicks, Joan; DeSander, Julie; Behrens, Jeff; Rueda, Bo R

Targeted therapeutics that can differentiate between normal and malignant tumor cells represent the ideal standard for the development of a successful anti-cancer strategy. The Sialyl-Thomsen-nouveau antigen (STn or Sialyl-Tn, also known as CD175s) is rarely seen in normal adult tissues, but it is abundantly expressed in many types of human epithelial cancers. We have identified novel antibodies that specifically target with high affinity the STn glycan independent of its carrier protein, affording the potential to recognize a wider array of cancer-specific sialylated proteins. A panel of murine monoclonal anti-STn therapeutic antibodies were generated and their binding specificity and efficacy were characterized in vitro and in in vivo murine cancer models. A subset of these antibodies were conjugated to monomethyl auristatin E (MMAE) to generate antibody-drug conjugates (ADCs). These ADCs demonstrated in vitro efficacy in STn-expressing cell lines and significant tumor growth inhibition in STn-expressing tumor xenograft cancer models with no evidence of overt toxicity.

Origins and Formation of Corporate Education in the USA

ERIC Educational Resources Information Center

Lytovchenko, Iryna

2015-01-01

The article analyzes the process of formation and development of corporate education in the USA in the first half of the twentieth century. It has been determined that the main prerequisites for the development of corporate education in the USA in the first half of the twentieth century were historical, socio-economic, political factors and…

Chapter 9: The rock coast of the USA

USGS Publications Warehouse

Hapke, Cheryl J.; Adams, Peter N.; Allan, Jonathan; Ashton, Andrew; Griggs, Gary B.; Hampton, Monty A.; Kelly, Joseph; Young, Adam P.

2014-01-01

The coastline of the USA is vast and comprises a variety of landform types including barrier islands, mainland beaches, soft bluffed coastlines and hard rocky coasts. The majority of the bluffed and rocky coasts are found in the northeastern part of the country (New England) and along the Pacific coast. Rocky and bluffed landform types are commonly interspersed along the coastline and occur as a result of relative lowering of sea level from tectonic or isostatic forcing, which can occur on timescales ranging from instantaneous to millenia. Recent research on sea cliffs in the contiguous USA has focused on a broad range of topics from documenting erosion rates to identifying processes and controls on morphology to prediction modelling. This chapter provides a detailed synthesis of recent and seminal research on rocky coast geomorphology along open-ocean coasts of the continental United States (USA).

Public Notice: Proposed Land Ban Exemption to Ineos USA LLC

EPA Pesticide Factsheets

Public notice for proposed Land Ban Exemption Reissuance, for Ineos USA LLC, to continue to inject hazardous waste deep underground in Allen County, OH. EPA plans to reissue a Land Ban Exemption for Ineos USA LLC in Allen Co., OH.

Measurements of PAN, PPN, and MPAN made during the 1994 and 1995 Nashville Intensives of the Southern Oxidant Study: Implications for regional ozone production from biogenic hydrocarbons

NASA Astrophysics Data System (ADS)

Roberts, James M.; Williams, Jonathan; Baumann, Karsten; Buhr, Martin P.; Goldan, Paul D.; Holloway, John; Hübler, Gerhard; Kuster, William C.; McKeen, Stuart A.; Ryerson, Thomas B.; Trainer, Michael; Williams, Eric J.; Fehsenfeld, Fredrick C.; Bertman, Steven B.; Nouaime, George; Seaver, Craig; Grodzinsky, Gil; Rodgers, Michael; Young, Valerie L.

1998-09-01

Isoprene and a variety of other reactive hydrocarbons are released in large quantities by vegetation in forested regions and are thought to participate in the NOx-catalyzed production of ozone, a serious air quality problem in North America and Europe [National Research Council, 1991]. The determination of the fraction of O3 formed from anthropogenic NOx and biogenic hydrocarbons (BHC) is a crucial step in the formulation of effective control strategies. Peroxymethacrylic nitric anhydride (MPAN, CH2C(CH3)C(O)OONO2) is formed almost entirely from the atmospheric oxidation of isoprene in the presence of NOx and is an excellent indicator of recent ozone production from isoprene and therefore biogenic hydrocarbons. Measurements are presented here of MPAN, peroxyacetic nitric anhydride (PAN, CH3C(O)OONO2), peroxypropionic nitric anhydride (PPN, CH3CH2C(O)OONO2) and ozone from separate data sets acquired during the 1994 and 1995 Nashville intensive studies of the Southern Oxidant Study. It was found that PAN, a general product of HC-NOx photochemistry, could be well represented as a simple linear combination of contributions from BHC and anthropogenic hydrocarbon (AHC) chemistries as indicated by MPAN and PPN, respectively. The PAN:MPAN ratios found to be characteristic of BHC-dominated chemistry ranged from 6 to 10. The PAN:PPN ratios found to be characteristic of AHC-dominated chemistry ranged from 5.8 to 7.4. These BHC and AHC attributions were used to estimate the contributions of anthropogenic and biogenic hydrocarbons to regional tropospheric ozone production, and substantial BHC-O3 (50-60 ppbv) was estimated in cases where high NOx from power plants was present in areas of high BHC emission. This estimation method provides direct evidence of significant photochemical ozone production from the oxidation of biogenic hydrocarbons in the presence of NOx.

Figure 12(a) Effects of Inclining Water. Figure 12(b) Sand. NASA document NASA-TN-D-56 An investigation to determine conditions under which downwash from VTOL aircraft will start surface erosion from various types of terrain

NASA Image and Video Library

1959-05-04

Figure 12(a) Effects of Inclining Water. Figure 12(b) Sand. NASA-TN-D-56 An investigation to determine conditions under which downwash from VTOL aircraft will start surface erosion from various types of terrain.

Water/magma mass fractions in phreatomagmatic eruption plumes - constraints from the Grímsvötn 2011 eruption

NASA Astrophysics Data System (ADS)

Gudmundsson, M. T.; Pálsson, F.; Thordarson, T.; Hoskuldsson, A.; Larsen, G.; Hognadottir, T.; Oddsson, B.; Oladottir, B. A.; Gudnason, J.

2014-12-01

Explosive interaction of magma and water leads to vaporization and introduces external water vapor to volcanic plumes. Theoretical considerations on the effect of external water magma ratio on volcanic plumes indicate that plume buoyancy should be enhanced by external water fractions up to at least 30%, while fractions reaching 40% should lead to plume collapse. The basaltic VEI 4 eruption of Grímsvötn in May 2011 produced a 15-20 km high eruption plume and over 100 km wide umbrella cloud. External water interacted with the magma and entered the plume from the melting out of a 100-150 m deep ice cauldron that had acquired a volume of 0.1 km3 at the end of the eruption. About 0.7 km3 of tephra was produced in the eruption whereof about half was erupted in phreatomagmatic phases and the other half in magmatic phases. During the dry, magmatic phases melting was apparently not fast enough to supply sufficient external water to the vents to control the style of activity. The only source of external water was the melting out of the ice cauldron since no changes took place in the level of the larger, subglacial lake in the center of the Grímsvötn caldera, and no meltwater was drained from the caldera. The eruption site therefore had little or no hydrological connection with the adjacent subglacial lake. Water remaining at the eruption site at the end of the eruption was miniscule compared to the amount of ice melted. Hence, most of the meltwater was vaporized and carried away as a part of the eruption plume. About one third of the thermal energy of the magma erupted was used to melt, heat up and vaporize water. A large part of this water was released from the plume through condensation and re-freezing, manifested in hail-rich tephra deposited out to several kilometers from the vent. The data indicate that the external water/tephra mass ratio in the phreatomagmatic phases was 20-25%, but similar to 5% for the predominantly magmatic phases.

Complete genome sequence of community-associated methicillin-resistant Staphylococcus aureus (strain USA400-0051), a prototype of the USA400 clone

PubMed Central

Côrtes, Marina Farrel; Costa, Maiana OC; Lima, Nicholas CB; Souza, Rangel C; Almeida, Luiz GP; Guedes, Luciane Prioli Ciapina; Vasconcelos, Ana TR; Nicolás, Marisa F; Figueiredo, Agnes MS

2017-01-01

Staphylococcus aureus subsp. aureus, commonly referred as S. aureus, is an important bacterial pathogen frequently involved in hospital- and community-acquired infections in humans, ranging from skin infections to more severe diseases such as pneumonia, bacteraemia, endocarditis, osteomyelitis, and disseminated infections. Here, we report the complete closed genome sequence of a community-acquired methicillin-resistant S. aureus strain, USA400-0051, which is a prototype of the USA400 clone. PMID:29091141

OBIS-USA: a data-sharing legacy of the census of marine life

USGS Publications Warehouse

Sedberry, G.R.; Fautin, D.G.; Feldman, M.; Fornwall, M.D.; Goldstein, P.; Guralnick, R.P.

2011-01-01

The United States Geological Survey's Biological Informatics Program hosts OBIS-USA, the US node of the Ocean Biogeographic Information System (OBIS). OBIS-USA gathers, coordinates, applies standard formats to, and makeswidely available data on biological collections in marine waters of the United States and other areas where US investigators have collected data and, in some instances, specimens. OBIS-USA delivers its data to OBIS international, which then delivers its data to the Global Biodiversity Information Facility (GBIF) and other Web portals for marine biodiversity data. OBIS-USA currently has 145 data sets from 36 participants, representing over 6.5 million occurrence records of over 83,000 taxa from more than 888,000 locations. OBIS-USA, a legacy of the decade-long (2001-2010) international collaborative Census of Marine Life enterprise, continues to add data, including those from ongoing Census projects. Among the many challenges in creating OBIS, including OBIS-USA, were developing a community of trust and shared valueamong data providers, and demonstrating to providers the value of making their data accessible to others. Challenges also posed by the diversity of data sets relevant tomarine biodiversity stored on thousands of computers, in a variety of formats, not all widely accessible, have been met in OBIS-USA by implementing a uniform standard and publishing platform that is easily accessible to a broad range of users.

Age at onset in bipolar I affective disorder in the USA and Europe.

PubMed

Bellivier, Frank; Etain, Bruno; Malafosse, Alain; Henry, Chantal; Kahn, Jean-Pierre; Elgrabli-Wajsbrot, Orly; Jamain, Stéphane; Azorin, Jean-Michel; Frank, Ellen; Scott, Jan; Grochocinski, Victoria; Kupfer, David J; Golmard, Jean-Louis; Leboyer, Marion

2014-07-01

To test for differences in reported age at onset (AAO) of bipolar I affective disorder in clinical samples drawn from Europe and the USA. Admixture analysis was used to identify the model best fitting the observed AAO distributions of two large samples of bipolar I patients from Europe and USA (n = 3616 and n = 2275, respectively). Theoretical AAO functions were compared between the two samples. The model best fitting the observed distribution of AAO in both samples was a mixture of three Gaussian distributions. The theoretical AAO functions of bipolar I disorder differed significantly between the European and USA populations, with further analyses indicating that (i) the proportion of patients belonging to the early-onset subgroup was higher in the USA sample (63 vs. 25%) and (ii) mean age at onset (±SD) in the early-onset subgroup was lower for the USA sample (14.5 ± 4.9 vs. 19 ± 2.7 years). The models best describing the reported AAO distributions of European and USA bipolar I patients were remarkably stable. The intermediate- and late-onset subgroups had similar characteristics in the two samples. However, the theoretical AAO function differed significantly between the USA and European samples due to the higher proportion of patients in the early-onset subgroup and the lower mean age-at-onset in the USA sample.

Life After USA300: The Rise and Fall of a Superbug

PubMed Central

2017-01-01

Abstract The community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) epidemic in the United States is largely attributable to the meteoric rise of a single clone, referred to as USA300. This strain not only spread across the United States in just a few years to become the predominant cause of staphylococcal disease, but it also appears to have increased the overall number of skin and soft-tissue infections (SSTIs), increasing the overall disease burden. While USA300 still constitutes a major public health burden, its prevalence may be decreasing in some parts of the United States. Other than an epidemic in South America due to a closely related strain, USA300 also seems to have been largely unable to establish itself as an endemic infection in other geographic locations. While there have been several hypotheses put forward to explain the enormous success of USA300, the reasons for its failures and its potential fall remain obscure. Far from being unique to USA300, the rise and fall of specific clones of S. aureus in human populations seems to be a common process that has occurred multiple times and in multiple locations. This review charts the rise of USA300 and the evidence that suggests that it may be in decline, and it considers how best to understand the future spread, containment, and possible extinction of CA-MRSA. PMID:28375517

Distribution, hosts and identification of Meloidogyne partityla in the USA

USDA-ARS?s Scientific Manuscript database

Pecan, Carya illinoensis, is an economically important nut crop and member of the Juglandaceae native to the southern USA. Discovered in South Africa in 1986, Meloidogyne partityla was first found infecting pecan in USA in 1996 and currently occurs in Texas, New Mexico, Georgia, Arizona, Oklahoma a...

Boston, Massachusetts, USA

NASA Technical Reports Server (NTRS)

1990-01-01

Under a light dusting of snow, the Boston, Massachusetts, USA (42.0N, 71.0W) area can be seen in great detail. Originally, the town site of Boston was on one of several islands offshore for defensive purposes. Over the years, however, ambitious public works projects have filled in between many of the islands to create a single municipality. This area is rich in early American history and much of it can be traced in this detailed overhead view.

78 FR 64020 - Manufacturer of Controlled Substances, Notice of Registration, Siegfried USA, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-25

..., Notice of Registration, Siegfried USA, LLC The Notice dated July 23, 2013, and published in the Federal Register on August 1, 2013, 78 FR 46613, page 18338, Siegfried (USA), LLC., 33 Industrial Park Road...), and Poppy Straw Concentrate (9670). On August 6, 2013, Siegfried (USA), LLC., subsequently withdrew...

Transmission of multidrug-resistant tuberculosis in the USA: a cross-sectional study.

PubMed

Moonan, Patrick K; Teeter, Larry D; Salcedo, Katya; Ghosh, Smita; Ahuja, Shama D; Flood, Jennifer; Graviss, Edward A

2013-09-01

Multidrug-resistant (MDR) tuberculosis is a potential threat to tuberculosis elimination, but the extent of MDR tuberculosis disease in the USA that is attributable to transmission within the country is unknown. We assessed transmission of MDR tuberculosis and potential contributing factors in the USA. In a cross-sectional study, clinical, demographic, epidemiological, and Mycobacterium tuberculosis genotype data were obtained during routine surveillance of all verified cases of MDR tuberculosis reported from eight states in the USA (California from Jan 1, 2007, to Dec 31, 2009; Texas from Jan 1, 2007, to March 31, 2009; and the states of Colorado, Maryland, Massachusetts, New York, Tennessee, and Washington from Jan 1, 2007 to Dec 31, 2008). In-depth interviews and health-record abstraction were done for all who consented to ascertain potential interpersonal connections. 168 cases of MDR tuberculosis were reported in the eight states during our study period. 92 individuals (55%) consented to in-depth interview. 20 (22%) of these individuals developed MDR tuberculosis as a result of transmission in the USA; a source case was identified for eight of them (9%). 20 individuals (22%) had imported active tuberculosis (ie, culture-confirmed disease within 3 months of entry into the USA). 38 (41%) were deemed to have reactivation of disease, of whom 14 (15%) had a known previous episode of tuberculosis outside the USA. Five individuals (5%) had documented treatment of a previous episode in the USA, and so were deemed to have relapsed. For nine cases (10%), insufficient evidence was available to definitively classify reason for presentation. About a fifth of cases of MDR tuberculosis in the USA can be linked to transmission within the country. Many individuals acquire MDR tuberculosis before entry into the USA. MDR tuberculosis needs to be diagnosed rapidly to reduce potential infectious periods, and clinicians should consider latent tuberculosis infection treatment

78 FR 30333 - Manufacturer of Controlled Substances; Notice of Registration; Siegfried (Usa), Llc.

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-22

...; Notice of Registration; Siegfried (Usa), Llc. By Notice dated November 19, 2012, and published in the Federal Register on November 27, 2012, 77 FR 70825, Siegfried (USA), LLC., 33 Industrial Park Road... 21 U.S.C. 823(a) and determined that the registration of Siegfried (USA), LLC., to manufacture the...

78 FR 64020 - Manufacturer of Controlled Substances; Notice of Registration; Siegfried USA, LLC

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-25

...; Notice of Registration; Siegfried USA, LLC By Notice dated May 22, 2013, and published in the Federal Register on May 30, 2013, 78 FR 32458, Siegfried USA, LLC., 33 Industrial Park Road, Pennsville, New Jersey... determined that the registration of Siegfried USA, LLC., to manufacture the listed basic class of controlled...

GLIMPSE: A GCAM-USA-based tool for supporting coordinated energy and environmental planning

EPA Science Inventory

GCAM-USA is an integrated assessment model, meaning that it simulates the interactions among human and earth systems. GCAM-USA is derived from GCAM, which represents the U.S. as one region within a 32-region global model. GCAM-USA subdivides the U.S. region into 50 states and the...

Suicidality, ethnicity and immigration in the USA.

PubMed

Borges, G; Orozco, R; Rafful, C; Miller, E; Breslau, J

2012-06-01

Suicide is the 11th leading cause of death in the USA. Suicide rates vary across ethnic groups. Whether suicide behavior differs by ethnic groups in the USA in the same way as observed for suicide death is a matter of current discussion. The aim of this report was to compare the lifetime prevalence of suicide ideation and attempt among four main ethnic groups (Asians, Blacks, Hispanics, and Whites) in the USA. Suicide ideation and attempts were assessed using the World Mental Health version of the Composite International Diagnostic Interview (WMH-CIDI). Discrete time survival analysis was used to examine risk for lifetime suicidality by ethnicity and immigration among 15 180 participants in the Collaborative Psychiatric Epidemiological Surveys (CPES), a group of cross-sectional surveys. Suicide ideation was most common among Non-Hispanic Whites (16.10%), least common among Asians (9.02%) and intermediate among Hispanics (11.35%) and Non-Hispanic Blacks (11.82%). Suicide attempts were equally common among Non-Hispanic Whites (4.69%), Hispanics (5.11%) and Non-Hispanic Blacks (4.15%) and less common among Asians (2.55%). These differences in the crude prevalence rates of suicide ideation decreased but persisted after control for psychiatric disorders, but disappeared for suicide attempt. Within ethnic groups, risk for suicidality was low among immigrants prior to migration compared to the US born, but equalized over time after migration. Ethnic differences in suicidal behaviors are explained partly by differences in psychiatric disorders and low risk prior to arrival in the USA. These differences are likely to decrease as the US-born proportion of Hispanics and Asians increases.

Sex-specific 99th percentiles derived from the AACC Universal Sample Bank for the Roche Gen 5 cTnT assay: Comorbidities and statistical methods influence derivation of reference limits.

PubMed

Gunsolus, Ian L; Jaffe, Allan S; Sexter, Anne; Schulz, Karen; Ler, Ranka; Lindgren, Brittany; Saenger, Amy K; Love, Sara A; Apple, Fred S

2017-12-01

Our purpose was to determine a) overall and sex-specific 99th percentile upper reference limits (URL) and b) influences of statistical methods and comorbidities on the URLs. Heparin plasma from 838 normal subjects (423 men, 415 women) were obtained from the AACC (Universal Sample Bank). The cobas e602 measured cTnT (Roche Gen 5 assay); limit of detection (LoD), 3ng/L. Hemoglobin A1c (URL 6.5%), NT-proBNP (URL 125ng/L) and eGFR (60mL/min/1.73m 2 ) were measured, along with identification of statin use, to better define normality. 99th percentile URLs were determined by the non-parametric (NP), Harrell-Davis Estimator (HDE) and Robust (R) methods. 355 men and 339 women remained after exclusions. Overall<50% of subjects had measureable concentrations ≥ LoD: 45.6% no exclusion, 43.5% after exclusion; compared to men: 68.1% no exclusion, 65.1% post exclusion; women: 22.7% no exclusion, 20.9% post exclusion. The statistical method used influenced URLs as follows: pre/post exclusion overall, NP 16/16ng/L, HDE 17/17ng/L, R not available; men NP 18/16ng/L, HDE 21/19ng/L, R 16/11ng/L; women NP 13/10ng/L, HDE 14/14ng/L, R not available. We demonstrated that a) the Gen 5 cTnT assay does not meet the IFCC guideline for high-sensitivity assays, b) surrogate biomarkers significantly lowers the URLs and c) statistical methods used impact URLs. Our data suggest lower sex-specific cTnT 99th percentiles than reported in the FDA approved package insert. We emphasize the importance of detailing the criteria used to include and exclude subjects for defining a healthy population and the statistical method used to calculate 99th percentiles and identify outliers. Copyright © 2017 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Recipe Modification Improves Food Safety Practices during Cooking of Poultry.

PubMed

Maughan, Curtis; Godwin, Sandria; Chambers, Delores; Chambers, Edgar

2016-08-01

Many consumers do not practice proper food safety behaviors when preparing food in the home. Several approaches have been taken to improve food safety behaviors among consumers, but there still is a deficit in actual practice of these behaviors. The objective of this study was to assess whether the introduction of food safety instructions in recipes for chicken breasts and ground turkey patties would improve consumers' food safety behaviors during preparation. In total, 155 consumers in two locations (Manhattan, KS, and Nashville, TN) were asked to prepare a baked chicken breast and a ground turkey patty following recipes that either did or did not contain food safety instructions. They were observed to track hand washing and thermometer use. Participants who received recipes with food safety instructions (n = 73) demonstrated significantly improved food safety preparation behaviors compared with those who did not have food safety instructions in the recipe (n = 82). In addition, the majority of consumers stated that they thought the recipes with instructions were easy to use and that they would be likely to use similar recipes at home. This study demonstrates that recipes could be a good source of food safety information for consumers and that they have the potential to improve behaviors to reduce foodborne illness.

Can genomics clarify the origins of Boreioglycaspis melaleucae in California, USA?

USDA-ARS?s Scientific Manuscript database

The Australian psyllid Boreioglycaspis melaleucae is a biological control agent of Melaleuca quinquenervia in Florida (USA) but was observed attacking M. quinquenervia trees in southern California (USA). Genotyping revealed the California population matched three of eight Australian haplotypes and ...

Marketing and Testosterone Treatment in the USA: A Systematic Review.

PubMed

Bandari, Jathin; Ayyash, Omar M; Emery, Sherry L; Wessel, Charles B; Davies, Benjamin J

2017-10-01

Testosterone replacement therapy (TRT) is currently approved by the Food and Drug Administration only for classic hypogonadism, although off-label indications have resulted in a dramatic expansion in prescriptions in the USA. Marketing may significantly affect prescriber behavior. To systematically review all available evidence on marketing and TRT in the USA. PubMed, Embase, and Scopus were searched up to July 2017 for all relevant publications reporting on assessments of the TRT market size, economic costs associated with hypogonadism, trends in TRT prescriptions, drug discontinuation rates, and advertising and sales efforts in the USA. Twenty retrospective studies were included in the final analysis. The market size for hypogonadism constitutes 5.6-76.8% of men in the USA, with the lower end of the range representing the strictest criteria for diagnosis. Men with a diagnosis of hypogonadism consume $14 118 in direct and indirect costs to the payer. Over the last 2 decades, TRT prescriptions have increased between 1.8- and 4-fold. After 1 yr, 80-85% of men discontinue TRT. There is an association between direct-to-consumer advertising and testosterone testing, TRT prescriptions, and TRT without testosterone testing. There is a high prevalence of misinformation on Internet advertising. Off-label indications have driven the dramatic expansion of TRT prescriptions over the last 2 decades. Direct-to-consumer advertising poses a unique challenge in the USA. Overtreatment can be avoided by applying strict diagnostic criteria for hypogonadism, which limits the addressable market for TRT. In this report, we reviewed the relationship between marketing and testosterone therapy in the USA. We found that many patients are prescribed testosterone without an appropriate diagnosis of hypogonadism, which may be related to the marketing efforts for off-label prescribing. Copyright © 2017 European Association of Urology. Published by Elsevier B.V. All rights reserved.

Dyslexia Laws in the USA

ERIC Educational Resources Information Center

Youman, Martha; Mather, Nancy

2013-01-01

Throughout the various states of the USA, the appropriate identification of dyslexia and the timely provision of interventions are characterized by variability and inconsistency. Several states have recognized the existence of this disorder and the well-established need for services. These states have taken proactive steps to implement laws and…

76 FR 59167 - Siemens Medical Solutions USA, Inc., Oncology Care Systems Division, Concord, CA; Siemens Medical...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-09-23

... Medical Solutions USA, Inc., Oncology Care Systems Division, Concord, CA; Siemens Medical Solutions USA... Solutions USA, Inc. (Siemens), Oncology Care Systems Division, Concord, California (subject firm). The...., Oncology Care Systems Division, Concord, California (TA-W-73,158) and Siemens Medical Solutions USA, Inc...

Transposon Tn10 contains two structural genes with opposite polarity between tetA and IS10R.

PubMed Central

Schollmeier, K; Hillen, W

1984-01-01

The nucleotide sequence of the central part of Tn10 has been determined from the rightmost HindIII site to IS10R. This sequence contains two open reading frames with opposite polarity. The in vivo transcription start points in this sequence have been determined by S1 mapping. These results define one minor and two major promoters. The transcription starts of the two major promoters are only 18 base pairs apart, and the transcripts show different polarity and overlap by 18 base pairs. The nucleotide sequence reveals two regions with palindromic symmetry which may serve as operators. Their possible involvement in the regulation of transcription of both genes is discussed. Taken together these results allow for a maximal coding capacity of 138 amino acids directed toward IS10R and 197 amino acids directed toward tetA. The possible function of these gene products is discussed. The accompanying article (Braus et al., J. Bacteriol. 160:504-509, 1984) presents evidence that these genes are expressed. Images PMID:6094471

The Tn Antigen-Specific Lectin from Ground Ivy Is an Insecticidal Protein with an Unusual Physiology1

PubMed Central

Wang, Weifang; Hause, Bettina; Peumans, Willy J.; Smagghe, Guy; Mackie, Anne; Fraser, Robin; Van Damme, Els J.M.

2003-01-01

Leaves of ground ivy (Glechoma hederacea) contain a lectin (called Gleheda) that is structurally and evolutionary related to the classical legume lectins. Screening of a population of wild plants revealed that Gleheda accounts for more than one-third of the total leaf protein in some clones, whereas it cannot be detected in other clones growing in the same environment. Gleheda is predominantly expressed in the leaves where it accumulates during early leaf maturation. The lectin is not uniformly distributed over the leaves but exhibits a unique localization pattern characterized by an almost exclusive confinement to a single layer of palisade parenchyma cells. Insect feeding trials demonstrated that Gleheda is a potent insecticidal protein for larvae of the Colorado potato beetle (Leptinotarsa decemlineata). Because Gleheda is not cytotoxic, it is suggested that the insecticidal activity is linked to the carbohydrate-binding specificity of the lectin, which as could be demonstrated by agglutination assays with different types of polyagglutinable human erythrocytes is specifically directed against the Tn antigen structure (N-acetylgalactosamine O-linked to serine or threonine residues of proteins). PMID:12857814

Tn5-Mob transposon mediated transfer of salt tolerance and symbiotic characteristics between Rhizobia genera.

PubMed

Yang, S; Wu, Z; Gao, W; Li, J

1993-01-01

Rhizobium meliloti 042B is a fast-growing, salt-tolerant and high efficiency nitrogen-fixing symbiont with alfalfa. Bradyrhizobium japonicum USDA110 grows slowly, and cannot grow in YMA medium containing 0.1M NaCl, but nodulates and fixed nitrogen efficiently with soybean. Eighty-six transconjugants, called SR, were obtained by inserting Tn5-Mob randomly into genomes of 042B using pSUP5011 and helper plasmid RP4. Selecting 4 SR strains randomly and introducing DNA fragment of SR into USDA110 with helper plasmid R68.45 by triparental mating, 106 transconjugants, called BSR, were constructed. Most of BSR strains had the fast-growing phenotype and could tolerate 0.3-0.5M NaCl generally. Some of them produced melanine. When soybean and alfalfa were inoculated with these transconjugants BSR, 47 out of 90 BSR were found to nodulate in both of these plants, but no nitrogenase activity was observed with alfalfa; 26 strains could only nodulate and fix nitrogen in soybean; 13 strains could nodulate in alfalfa but did not fix nitrogen; 4 strains failed to nodulate in either soybean or alfalfa. Among them, 4 transconjugants which tolerated and fixed nitrogen efficiently in soybean were constructed.

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

A NASA staff member discusses planets with attendees of the USA Science and Engineering Festival, which took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

agr-Dependent Interactions of Staphylococcus aureus USA300 with Human Polymorphonuclear Neutrophils

PubMed Central

Pang, Yun Yun; Schwartz, Jamie; Thoendel, Matthew; Ackermann, Laynez W.; Horswill, Alexander R.; Nauseef, William M.

2010-01-01

The emergence of serious infections due to community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has fueled interest in the contributions of specific staphylococcal virulence factors to clinical disease. To assess the contributions of agr-dependent factors to the fate of organisms in polymorphonuclear neutrophils (PMN), we examined the consequences for organism and host cells of feeding PMN with wild-type CA-MRSA (LAC) or CA-MRSA (LAC agr KO) at different multiplicities of infection (MOIs). Phagocytosed organisms rapidly increased the transcription of RNAIII in a time- and MOI-dependent fashion; extracellular USA300 (LAC) did not increase RNAIII expression despite having the capacity to respond to autoinducing peptide-enriched culture medium. HOCl-mediated damage and intracellular survival were the same in the wild-type and USA300 (LAC agr KO). PMN lysis by ingested USA300 (LAC) was time- and MOI-dependent and, at MOIs >1, required α-hemolysin (hla) as USA300 (LAC agr KO) and USA300 (LAC hla KO) promoted PMN lysis only at high MOIs. Taken together, these data demonstrate activation of the agr operon in human PMN with the subsequent production of α-hemolysin and PMN lysis. The extent to which these events in the phagosomes of human PMN contribute to the increased morbidity and mortality of infections with USA300 (LAC) merits further study. PMID:20829608

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

A NASA staff member describes landing technology to an attendee of the USA Science and Engineering Festival, which took place at the Washington Convention Center in Washington, DC on April 26 and 27, 2014. Photo Credit: (NASA/Aubrey Gemignani)

Comparative study of scientific publications in orthopedics journals originating from USA, Japan and China (2000-2012).

PubMed

Lao, Li-Feng; Daubs, Michael David; Phan, Kevin H; Wang, Jeffrey C

2013-11-01

To compare orthopedics publications from USA, Japan and China. Scientific papers belong to ''Orthopedics'' category of Science Citation Index Expanded subject categories were retrieved from the "PubMed'' and ''Web of Knowledge'' online databases. In the field of orthopedics, the annual number increased significantly from 2000 to 2012 in the three countries (p<0.001). The share of articles increased significantly in China, but decreased significantly in Japan and USA (p<0.05). In 2012, USA contributed 35.3% of the total world output in orthopedics field and ranked 1st; Japan contributed 5.9% and ranked 4th; China contributed 5.2% and ranked 5th. Publications from USA had the highest accumulated IFs and the highest total citations of articles (USA > Japan > China, p<0.001). Average IF from USA was much higher than Japan and China (p<0.001). USA published the most articles in the top ten orthopedics journals (USA (14355) > Japan (1702) > China (487), p<0.01). Although China has undergone significant increase in annual number and percentage of scientific publication in orthopedics journals, it still lags far behind USA and Japan in the field of orthopedics in terms of quantity and quality.

High Rates of Staphylococcus aureus USA400 Infection, Northern Canada

PubMed Central

Golding, George R.; Levett, Paul N.; McDonald, Ryan R.; Irvine, James; Quinn, Brian; Nsungu, Mandiangu; Woods, Shirley; Khan, Mohammad; Ofner-Agostini, Marianna

2011-01-01

Surveillance of Staphylococcus aureus infections in 3 northern remote communities of Saskatchewan was undertaken. Rates of methicillin-resistant infections were extremely high (146–482/10,000 population), and most (98.2%) were caused by USA400 strains. Although USA400 prevalence has diminished in the United States, this strain is continuing to predominate throughout many northern communities in Canada. PMID:21470471

78 FR 4400 - Eni USA Gas Marketing LLC; Application for Blanket Authorization To Export Previously Imported...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-22

... DEPARTMENT OF ENERGY [FE Docket No. 12-161-LNG] Eni USA Gas Marketing LLC; Application for Blanket..., by Eni USA Gas Marketing LLC (Eni USA Gas Marketing), requesting blanket authorization to export... U.S. law or policy. Eni USA Gas Marketing is requesting this authorization both on its own behalf...

An economic evaluation of alternative biofuel deployment scenarios in the USA

DOE Office of Scientific and Technical Information (OSTI.GOV)

Oladosu, Gbadebo

Energy market conditions have shifted dramatically since the USA renewable fuel standards (RFS1 in 2005; RFS2 in 2007) were enacted. The USA has transitioned from an increasing dependence on oil imports to abundant domestic oil production. In addition, increases in the use of ethanol, the main biofuel currently produced in the USA, is now limited by the blend wall constraint. Given this, the current study evaluates alternative biofuel deployment scenarios in the USA, accounting for changes in market conditions. The analysis is performed with a general equilibrium model that reflects the structure of the USA biofuel market as the transitionmore » to advanced biofuel begins. Results suggest that ethanol consumption would increase, albeit slowly, if current biofuel deployment rates of about 10% are maintained as persistently lower oil prices lead to a gradual increase in the consumption of liquid transportation fuels. Without the blend wall constraint, this study finds that the overall economic impact of a full implementation of the USA RFS2 policy is largely neutral before 2022. However, the economic impacts become slightly negative under the blend wall constraint since more expensive bio-hydrocarbons are needed to meet the RFS2 mandates. Results for a scenario with reduced advanced biofuel deployment based on current policy plans show near neutral economic impacts up to 2027. This scenario is also consistent with another scenario where the volume of bio-hydrocarbons deployed is reduced to adjust for its higher cost and energy content relative to deploying the mandated RFS2 advanced biofuel volumes as ethanol. The important role of technological change is demonstrated under pioneer and accelerated technology scenarios, with the latter leading to neutral or positive economic effects up to 2023 under most blend wall scenarios. Here, all scenarios evaluated in this study are found to have positive long-term economic benefits for the USA economy.« less

An economic evaluation of alternative biofuel deployment scenarios in the USA

DOE PAGES

Oladosu, Gbadebo

2017-05-03

Energy market conditions have shifted dramatically since the USA renewable fuel standards (RFS1 in 2005; RFS2 in 2007) were enacted. The USA has transitioned from an increasing dependence on oil imports to abundant domestic oil production. In addition, increases in the use of ethanol, the main biofuel currently produced in the USA, is now limited by the blend wall constraint. Given this, the current study evaluates alternative biofuel deployment scenarios in the USA, accounting for changes in market conditions. The analysis is performed with a general equilibrium model that reflects the structure of the USA biofuel market as the transitionmore » to advanced biofuel begins. Results suggest that ethanol consumption would increase, albeit slowly, if current biofuel deployment rates of about 10% are maintained as persistently lower oil prices lead to a gradual increase in the consumption of liquid transportation fuels. Without the blend wall constraint, this study finds that the overall economic impact of a full implementation of the USA RFS2 policy is largely neutral before 2022. However, the economic impacts become slightly negative under the blend wall constraint since more expensive bio-hydrocarbons are needed to meet the RFS2 mandates. Results for a scenario with reduced advanced biofuel deployment based on current policy plans show near neutral economic impacts up to 2027. This scenario is also consistent with another scenario where the volume of bio-hydrocarbons deployed is reduced to adjust for its higher cost and energy content relative to deploying the mandated RFS2 advanced biofuel volumes as ethanol. The important role of technological change is demonstrated under pioneer and accelerated technology scenarios, with the latter leading to neutral or positive economic effects up to 2023 under most blend wall scenarios. Here, all scenarios evaluated in this study are found to have positive long-term economic benefits for the USA economy.« less

Houston/Galveston, Texas, USA

NASA Image and Video Library

1991-09-18

In this view of Houston/Galveston, Texas, USA (29.5N, 95.5W), heavy spring rains emphasize the several bodies of water in the area. Even though partially cloud covered, the progressive nature of the Houston highway and freeway system can easily be observed in this highly detailed view. To the south, the NASA, Clear Lake area just off of Galveston Bay can easily be seen. In the center, is the downtown business district.

Perspective on IUPAP-ICWIP conferences and USA Participation

NASA Astrophysics Data System (ADS)

White, Herman

2015-04-01

Starting in 1999, the (International Union of Pure and Applied Physics) IUPAP, General Assembly, passed a resolution to form an IUPAP Working Group on Women in Physics. This lead to a number of international conferences that focused on analyzing the then current status of and progress in promoting women in physics for each country and world wide as well as sharing physics research progress and participation. I was twice a member of the USA delegation and participated in two of the last three of these conferences. I will present a perspective on the USA participation and contribution to the efforts of the conferences.

Colorectal cancer survival in the USA and Europe: a CONCORD high-resolution study.

PubMed

Allemani, Claudia; Rachet, Bernard; Weir, Hannah K; Richardson, Lisa C; Lepage, Côme; Faivre, Jean; Gatta, Gemma; Capocaccia, Riccardo; Sant, Milena; Baili, Paolo; Lombardo, Claudio; Aareleid, Tiiu; Ardanaz, Eva; Bielska-Lasota, Magdalena; Bolick, Susan; Cress, Rosemary; Elferink, Marloes; Fulton, John P; Galceran, Jaume; Gózdz, Stanislaw; Hakulinen, Timo; Primic-Zakelj, Maja; Rachtan, Jadwiga; Diba, Chakameh Safaei; Sánchez, Maria-José; Schymura, Maria J; Shen, Tiefu; Tagliabue, Giovanna; Tumino, Rosario; Vercelli, Marina; Wolf, Holly J; Wu, Xiao-Cheng; Coleman, Michel P

2013-09-10

To assess the extent to which stage at diagnosis and adherence to treatment guidelines may explain the persistent differences in colorectal cancer survival between the USA and Europe. A high-resolution study using detailed clinical data on Dukes' stage, diagnostic procedures, treatment and follow-up, collected directly from medical records by trained abstractors under a single protocol, with standardised quality control and central statistical analysis. 21 population-based registries in seven US states and nine European countries provided data for random samples comprising 12 523 adults (15-99 years) diagnosed with colorectal cancer during 1996-1998. Logistic regression models were used to compare adherence to 'standard care' in the USA and Europe. Net survival and excess risk of death were estimated with flexible parametric models. The proportion of Dukes' A and B tumours was similar in the USA and Europe, while that of Dukes' C was more frequent in the USA (38% vs 21%) and of Dukes' D more frequent in Europe (22% vs 10%). Resection with curative intent was more frequent in the USA (85% vs 75%). Elderly patients (75-99 years) were 70-90% less likely to receive radiotherapy and chemotherapy. Age-standardised 5-year net survival was similar in the USA (58%) and Northern and Western Europe (54-56%) and lowest in Eastern Europe (42%). The mean excess hazard up to 5 years after diagnosis was highest in Eastern Europe, especially among elderly patients and those with Dukes' D tumours. The wide differences in colorectal cancer survival between Europe and the USA in the late 1990s are probably attributable to earlier stage and more extensive use of surgery and adjuvant treatment in the USA. Elderly patients with colorectal cancer received surgery, chemotherapy or radiotherapy less often than younger patients, despite evidence that they could also have benefited.

78 FR 25304 - Siemens Medical Solutions, USA, Inc., Oncology Care Systems (Radiation Oncology), Including On...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-30

..., USA, Inc., Oncology Care Systems (Radiation Oncology), Including On-Site Leased Workers From Source... Medical Solutions, USA, Inc., Oncology Care Systems (Radiation Oncology), including on- site leased... of February 2013, Siemens Medical Solutions, USA, Inc., Oncology Care Systems (Radiation Oncology...

Exposing some important barriers to health care access in the rural USA.

PubMed

Douthit, N; Kiv, S; Dwolatzky, T; Biswas, S

2015-06-01

To review research published before and after the passage of the Patient Protection and Affordable Care Act (2010) examining barriers in seeking or accessing health care in rural populations in the USA. This literature review was based on a comprehensive search for all literature researching rural health care provision and access in the USA. Pubmed, Proquest Allied Nursing and Health Literature, National Rural Health Association (NRHA) Resource Center and Google Scholar databases were searched using the Medical Subject Headings (MeSH) 'Rural Health Services' and 'Rural Health.' MeSH subtitle headings used were 'USA,' 'utilization,' 'trends' and 'supply and distribution.' Keywords added to the search parameters were 'access,' 'rural' and 'health care.' Searches in Google Scholar employed the phrases 'health care disparities in the USA,' inequalities in 'health care in the USA,' 'health care in rural USA' and 'access to health care in rural USA.' After eliminating non-relevant articles, 34 articles were included. Significant differences in health care access between rural and urban areas exist. Reluctance to seek health care in rural areas was based on cultural and financial constraints, often compounded by a scarcity of services, a lack of trained physicians, insufficient public transport, and poor availability of broadband internet services. Rural residents were found to have poorer health, with rural areas having difficulty in attracting and retaining physicians, and maintaining health services on a par with their urban counterparts. Rural and urban health care disparities require an ongoing program of reform with the aim to improve the provision of services, promote recruitment, training and career development of rural health care professionals, increase comprehensive health insurance coverage and engage rural residents and healthcare providers in health promotion. Copyright © 2015 The Royal Society for Public Health. Published by Elsevier Ltd. All rights

Homeschooling as an Alternative Form of Educational Provision in South Africa and the USA

ERIC Educational Resources Information Center

de Waal, Esther; Theron, Tinie

2003-01-01

This paper studies homeschooling as an alternative form of educational provision in South Africa and USA to determine what knowledge and experiences from research on homeschooling in the USA may be relevant to the South Africa situation. Homeschooling in the USA has a sound legal foundation and has become an acceptable educational alternative.…

Current situation of development of petroleum substituting energies (USA)

NASA Astrophysics Data System (ADS)

1993-03-01

Trends in development of petroleum substituting energies in the U.S.A. are described. Among non-fossil fuel based energies currently available, nuclear power generation (7%), biomass power generation (4%), and hydraulic power generation (3%) account for a large part. The future for the nuclear energy is opaque. Biomasses are anticipated to be the largest regenerative energy source. Solar energy was regarded to be a future energy source, but its cost effect is not still good. While geothermal power generation produces 0.1% of the entire energy, its future is bright. Ocean energies of all types of form such as sea water thermal energy conversion and wave energy were not treated as a substituting energy in the U.S.A. Multi-fuel vehicles using gasoline, methanol, and ethanol are estimated to account for 25% of vehicle operations in the U.S.A. by 2000. Electric vehicles for practical use would be a hybrid type combining electric motors and gasoline engines.

Arab American Parents' Perceptions of Their Children's Experience in the USA: A Qualitative Study

ERIC Educational Resources Information Center

Ahmad, Jamal

2017-01-01

The purpose of this study was to examine Arab American parents' perceptions of difficulties their children experience in the USA. Both Arab American children born outside the USA who later moved to the country and children born in the USA to Arab parents were examined. Fifty Arab American families were interviewed in two areas in the State of…

Methodology in Training Future Technology and Engineering Teachers in the USA

ERIC Educational Resources Information Center

Androshchuk, Iryna; Androshchuk, Ihor

2017-01-01

In the article, the defined problem has been justified and the significance of studying foreign experience in training future technology and engineering teachers in the USA has been determined. Particular attention has been paid to explanation of methods and forms of organization of future technology and engineering teachers' training in the USA.…

75 FR 22411 - Fonterra (USA) Inc.; Filing of Food Additive Petition

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-28

...] Fonterra (USA) Inc.; Filing of Food Additive Petition AGENCY: Food and Drug Administration, HHS. ACTION... a petition proposing that the food additive regulations be amended to provide for the safe use of... that a food additive petition (FAP 0A4781) has been filed by Fonterra (USA), Inc., c/o Burdock Group...

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

Attendees visit NASA exhibits during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

Astronaut Rex Walheim spoke at the USA Science and Engineering Festival on April 25, 2014. The festival was open to the public on April 26 and 27, 2014 and was held at the Washington Convention Center in Washington, DC. Photo Credit: (NASA/Aubrey Gemignani)

Comprehensive Osteopathic Medical Licensing Examination-USA level 1 and level 2-cognitive evaluation preparation and outcomes.

PubMed

Maholtz, Danielle E; Erickson, Michael J; Cymet, Tyler

2015-04-01

The Comprehensive Osteopathic Medical Licensing Examination-USA (COMLEX-USA) assesses the competence of osteopathic physicians in training. It is designed to protect the public by setting minimum competence standards. All osteopathic medical students must pass COMLEX-USA Level 1, Level 2-Cognitive Evaluation, and Level 2-Performance Evaluation before being allowed to graduate from an osteopathic medical school. Residency training programs use COMLEX-USA scores as a major factor in deciding whom they will interview and admit into their programs. In addition, colleges of osteopathic medicine use student COMLEX-USA scores as an external assessment of their success in educating students. Because COMLEX-USA is a high-stakes examination series, it is important to understand predictive factors for performance. The authors review the literature on the relationship between COMLEX-USA scores and correlated student variables. Results from the Council on Osteopathic Student Government Presidents' survey on students' preparation methods and performance are also provided.

The Regionalization of National-Scale SPARROW Models for Stream Nutrients

USGS Publications Warehouse

Schwarz, G.E.; Alexander, R.B.; Smith, R.A.; Preston, S.D.

2011-01-01

This analysis modifies the parsimonious specification of recently published total nitrogen (TN) and total phosphorus (TP) national-scale SPAtially Referenced Regressions On Watershed attributes models to allow each model coefficient to vary geographically among three major river basins of the conterminous United States. Regionalization of the national models reduces the standard errors in the prediction of TN and TP loads, expressed as a percentage of the predicted load, by about 6 and 7%. We develop and apply a method for combining national-scale and regional-scale information to estimate a hybrid model that imposes cross-region constraints that limit regional variation in model coefficients, effectively reducing the number of free model parameters as compared to a collection of independent regional models. The hybrid TN and TP regional models have improved model fit relative to the respective national models, reducing the standard error in the prediction of loads, expressed as a percentage of load, by about 5 and 4%. Only 19% of the TN hybrid model coefficients and just 2% of the TP hybrid model coefficients show evidence of substantial regional specificity (more than ??100% deviation from the national model estimate). The hybrid models have much greater precision in the estimated coefficients than do the unconstrained regional models, demonstrating the efficacy of pooling information across regions to improve regional models. ?? 2011 American Water Resources Association. This article is a U.S. Government work and is in the public domain in the USA.

High youth access to movies that contain smoking in Europe compared with the USA.

PubMed

Hanewinkel, Reiner; Sargent, James D; Karlsdóttir, Sólveig; Jónsson, Stefán Hrafn; Mathis, Federica; Faggiano, Fabrizio; Poelen, Evelien A P; Scholte, Ron; Florek, Ewa; Sweeting, Helen; Hunt, Kate; Morgenstern, Matthis

2013-07-01

Based on evidence that exposure to smoking in movies is associated with adolescent smoking, the WHO has called on countries to assign a rating that restricts youth access to such movies. To evaluate youth access to movies that portray smoking in European countries and compare with that in the USA. The authors identified the most commercially successful movies screened in six European countries (Germany, Iceland, Italy, the Netherlands, Poland and UK) and the USA between 2004 and 2009. The authors coded the 464 movies that were screened in both Europe and the USA according to whether or not they portrayed smoking. 87% of the movies were 'youth' rated in Europe (ratings board classification as suitable for those younger than 16 years) compared to only 67% in the USA (suitable for those younger than 17 years). Smoking was portrayed in 319 (69%) movies. 85% of the movies that portrayed smoking were 'youth' rated in Europe compared with only 59% in the USA (p<0.001). Tobacco imagery is still common in popular films shown in European countries and the USA. None of the seven countries examined followed the WHO recommendations on restricting youth access to movies that portray smoking. Compared to the USA, European youths have access to substantially more movies in general, and this gives them access to more movies that portray smoking in particular.

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-22

Visitors crowd the NASA exhibits during the USA Science and Engineering Festival, Saturday, Oct. 23, 2010, on the National Mall in Washington. NASA, joined with more than 500 science organizations this weekend to inspire the next generation of scientists and engineers during the first national science and engineering festival held in the nation's capital. Photo Credit: (NASA/Paul E. Alers)

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-22

Visitors to the USA Science and Engineering Festival look over the many exhibits, Saturday, Oct. 23, 2010, at Freedom Plaza in Washington. NASA, joined with more than 500 science organizations this weekend to inspire the next generation of scientists and engineers during the first national science and engineering festival held in the nation's capital. Photo Credit: (NASA/Paul E. Alers)

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-22

Participants look through telescopes to observe the Sun during the USA Science and Engineering Festival, Saturday, Oct. 23, 2010, at Freedom Plaza in Washington. NASA, joined with more than 500 science organizations this weekend to inspire the next generation of scientists and engineers during the first national science and engineering festival held in the nation's capital. Photo Credit: (NASA/Paul E. Alers)

University-Industry Collaboration in China and the USA: A Bibliometric Comparison.

PubMed

Zhou, Ping; Tijssen, Robert; Leydesdorff, Loet

2016-01-01

In this study, university-industry collaborations in China and the USA are analyzed in terms of co-authored publications indexed in the Web of Science (WoS). Results show a wide gap between China and the USA: Chinese universities are much less active in collaborations with industry in terms of either publication productivity or collaboration intensity. In selecting local and foreign industrial partners, however, more variation exists among Chinese universities than among US universities. The US system is domestically oriented more than that of China. In the USA, the intensity of university-industry collaboration is determined by research quality, whereas in China this is not the case. In both China and the USA, distance is not critical for the establishment of domestic university-industry collaboration. A high correlation is found between productivity indicators including total publications and university-industry co-authored publications. However, the productivity indicators are less correlated with the intensity of university-industry collaboration. Large research universities with strong ties to domestic industry play critical roles in both national publication systems.

University-Industry Collaboration in China and the USA: A Bibliometric Comparison

PubMed Central

2016-01-01

In this study, university-industry collaborations in China and the USA are analyzed in terms of co-authored publications indexed in the Web of Science (WoS). Results show a wide gap between China and the USA: Chinese universities are much less active in collaborations with industry in terms of either publication productivity or collaboration intensity. In selecting local and foreign industrial partners, however, more variation exists among Chinese universities than among US universities. The US system is domestically oriented more than that of China. In the USA, the intensity of university-industry collaboration is determined by research quality, whereas in China this is not the case. In both China and the USA, distance is not critical for the establishment of domestic university-industry collaboration. A high correlation is found between productivity indicators including total publications and university-industry co-authored publications. However, the productivity indicators are less correlated with the intensity of university-industry collaboration. Large research universities with strong ties to domestic industry play critical roles in both national publication systems. PMID:27832084

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

Attendees learn about packing for space travel during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

An overview of EU and USA intestinal transplant current activity.

PubMed

Lauro, A; Panaro, F; Iyer, K R

2017-04-01

To report the current activity of intestinal transplantation in Europe (EU) and Unites States of America (USA), underlining outcomes in the last 5 years and discussing possible trends. Data review of results was performed through analysis of ITR and UNOS registries, Eurotransplant and newsletter transplant reports, congress abstracts, international published literature, personal communications and hospital web sites. The absence in Europe of a sole organization collecting donors and the presence of many low-volume centers (less than 5 cases/year) makes the difference with USA: in the last 5 years (2010-2014), 222 intestinal/multivisceral transplants have been performed in EU countries (most of them in the UK), while in USA, the number of transplants achieved 634 procedures in the same period of time. Waiting list mortality remains unacceptable in both continents. Improved short-term results, with over 80% survival at 1 year, have been achieved in the busiest transplant centers likely due to immune-induction agents, more recently to innovative cross match strategies and optimizing organ allocation, but long term outcomes are still inferior to other organ transplants. Most long-term survivors were reintegrated to society with self-sustained socioeconomic status. The economic burden for the society is high and related costs are different between USA and EU (and inside Europe between member state's health-care systems), but cost-effectiveness for intestinal transplantation still needs to be proved. Overall intestinal transplantation continues to develop in EU and USA together with surgical and medical rehabilitation of patients affected by short gut syndrome. Copyright © 2017 Elsevier Masson SAS. All rights reserved.

Timing Calibration of the USA Experiment

NASA Astrophysics Data System (ADS)

Ray, P. S.; Wood, K. S.; Bandyopadhyay, R. M.; Fritz, G.; Hertz, P.; Kowalski, M. P.; Lovellette, M. N.; Wolff, M. T.; Yentis, D.; Bloom, E.; Focke, W.; Giebels, B.; Godfrey, G.; Reilly, K. T.; Saz Parkinson, P.; Shabad, G.; Scargle, J.; Backer, D.; Somer, A.; USA Experiment Science Working Group

2000-10-01

The USA Experiment on ARGOS is an X-ray proportional counter timing experiment, launched in January 1999, which is carrying out a broad program studying X-ray binaries, rotation-powered pulsars, and other bright X-ray sources. Photon events are time tagged to an accuracy of 2 μ s by reference to an onboard GPS receiver built by Boeing (then Rockwell International). Unfortunately, the GPS receiver has an anomaly that causes it to drop out of lock after a few hours. We describe the procedures developed to work around the GPS anomaly and recover accurate absolute time. Simultaneous observations of several rotation-powered pulsars with RXTE were made for comparison with contemporaneous radio timing measurements and to explore time transfer from satellite to satellite. Basic research in X-ray Astronomy at the Naval Research Laboratory is supported by NRL/ONR. Work on USA at SLAC is supported by Department of Energy contract DE-AC03-76SF00515.

GETTING TO KNOW YOU. ERIC PASLAY.

PubMed

Rosenfeld, Cheryl A

2017-03-01

If you're a country music fan, you've probably heard of Eric Paslay; 33, a Nashville-based platinum-selling singer/songwriter. After starting to play guitar at age 15, Paslay knew by 18 that music was his path. In 2011, he signed with EMI Records Nashville, and since then he has had five number one hits, including four recorded by other artists such as Rascal Flatts as well as "Friday Night," the lead single from his self-titled debut album. His latest album, "Dressed in Black," includes the single "Angels in This Town."

78 FR 5500 - Manufacturer of Controlled Substances, Notice of Application; Siegfried (USA)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-25

... DEPARTMENT OF JUSTICE Drug Enforcement Administration Manufacturer of Controlled Substances, Notice of Application; Siegfried (USA) Pursuant to Sec. 1301.33(a), Title 21 of the Code of Federal Regulations (CFR), this is notice that on June 19, 2012, Siegfried (USA), 33 Industrial Park Road, Pennsville...

Carbon storage and sequestration by urban trees in the USA

Treesearch

David J. Nowak; Daniel E. Crane

2002-01-01

Based on field data from 10 USA cities and national urban tree cover data, it is estimated that urban trees in the coterminous USA currently store 700 million tonnes of carbon ($14,300 million value) with a gross carbon sequestration rate of 22.8 million tC/yr ($460 rnillion/year). Carbon storage within cities ranges From 1.2 million tC in New York, NY, to 19,300 tC in...

Babesia microti infection, eastern Pennsylvania, USA.

PubMed

Acosta, Marcela E Perez; Ender, Peter T; Smith, Erin M; Jahre, Jeffrey A

2013-07-01

Infection with Babesia microti has not been well-described in eastern Pennsylvania, USA, despite the vector of this organism being prevalent. We report 3 cases of babesiosis in eastern Pennsylvania in persons without recent travel outside the region or history of blood transfusions, suggesting emergence of this infection.

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

Astronaut Rex Walheim spoke at the USA Science and Engineering Festival on April 25, 2014 at the NASA Stage. The festival was open to the public on April 26 and 27, 2014 and was held at the Washington Convention Center in Washington, DC. Photo Credit: (NASA/Aubrey Gemignani)

Colorectal cancer survival in the USA and Europe: a CONCORD high-resolution study

PubMed Central

Allemani, Claudia; Rachet, Bernard; Weir, Hannah K; Richardson, Lisa C; Lepage, Côme; Faivre, Jean; Gatta, Gemma; Capocaccia, Riccardo; Sant, Milena; Baili, Paolo; Lombardo, Claudio; Aareleid, Tiiu; Ardanaz, Eva; Bielska-Lasota, Magdalena; Bolick, Susan; Cress, Rosemary; Elferink, Marloes; Fulton, John P; Galceran, Jaume; Góźdź, Stanisław; Hakulinen, Timo; Primic-Žakelj, Maja; Rachtan, Jadwiga; Diba, Chakameh Safaei; Sánchez, Maria-José; Schymura, Maria J; Shen, Tiefu; Tagliabue, Giovanna; Tumino, Rosario; Vercelli, Marina; Wolf, Holly J; Wu, Xiao-Cheng; Coleman, Michel P

2013-01-01

Objectives To assess the extent to which stage at diagnosis and adherence to treatment guidelines may explain the persistent differences in colorectal cancer survival between the USA and Europe. Design A high-resolution study using detailed clinical data on Dukes’ stage, diagnostic procedures, treatment and follow-up, collected directly from medical records by trained abstractors under a single protocol, with standardised quality control and central statistical analysis. Setting and participants 21 population-based registries in seven US states and nine European countries provided data for random samples comprising 12 523 adults (15–99 years) diagnosed with colorectal cancer during 1996–1998. Outcome measures Logistic regression models were used to compare adherence to ‘standard care’ in the USA and Europe. Net survival and excess risk of death were estimated with flexible parametric models. Results The proportion of Dukes’ A and B tumours was similar in the USA and Europe, while that of Dukes’ C was more frequent in the USA (38% vs 21%) and of Dukes’ D more frequent in Europe (22% vs 10%). Resection with curative intent was more frequent in the USA (85% vs 75%). Elderly patients (75–99 years) were 70–90% less likely to receive radiotherapy and chemotherapy. Age-standardised 5-year net survival was similar in the USA (58%) and Northern and Western Europe (54–56%) and lowest in Eastern Europe (42%). The mean excess hazard up to 5 years after diagnosis was highest in Eastern Europe, especially among elderly patients and those with Dukes’ D tumours. Conclusions The wide differences in colorectal cancer survival between Europe and the USA in the late 1990s are probably attributable to earlier stage and more extensive use of surgery and adjuvant treatment in the USA. Elderly patients with colorectal cancer received surgery, chemotherapy or radiotherapy less often than younger patients, despite evidence that they could also have benefited

Public Notice documents for OXY USA, Inc

EPA Pesticide Factsheets

EPA is seeking public comments on a proposed settlement agreement with OXY USA, Inc. EPA will not make a final decision on whether to enter the agreement until after considering public comments on the potential agreement.

High youth access to movies that contain smoking in Europe compared with the USA

PubMed Central

Hanewinkel, Reiner; Sargent, James D; Karlsdóttir, Sólveig; Jónsson, Stefán Hrafn; Mathis, Federica; Faggiano, Fabrizio; Poelen, Evelien A P; Scholte, Ron; Florek, Ewa; Sweeting, Helen; Hunt, Kate; Morgenstern, Matthis

2013-01-01

Background Based on evidence that exposure to smoking in movies is associated with adolescent smoking, the WHO has called on countries to assign a rating that restricts youth access to such movies. Objective To evaluate youth access to movies that portray smoking in European countries and compare with that in the USA. Methods The authors identified the most commercially successful movies screened in six European countries (Germany, Iceland, Italy, the Netherlands, Poland and UK) and the USA between 2004 and 2009. The authors coded the 464 movies that were screened in both Europe and the USA according to whether or not they portrayed smoking. Results 87% of the movies were ‘youth’ rated in Europe (ratings board classification as suitable for those younger than 16 years) compared to only 67% in the USA (suitable for those younger than 17 years). Smoking was portrayed in 319 (69%) movies. 85% of the movies that portrayed smoking were ‘youth’ rated in Europe compared with only 59% in the USA (p<0.001). Conclusions Tobacco imagery is still common in popular films shown in European countries and the USA. None of the seven countries examined followed the WHO recommendations on restricting youth access to movies that portray smoking. Compared to the USA, European youths have access to substantially more movies in general, and this gives them access to more movies that portray smoking in particular. PMID:22184208

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-22

Visitors to the USA Science and Engineering Festival look on at one of the many exhibits, Saturday, Oct. 23, 2010, on the National Mall in Washington. NASA, joined with more than 500 science organizations this weekend to inspire the next generation of scientists and engineers during the first national science and engineering festival held in the nation's capital. Photo Credit: (NASA/Paul E. Alers)

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-23

Young visitors to the inaugural USA Science and Engineering Festival at the National Mall in Washington, D.C., learn about the life cycle of a star at an exhibit sponsored by the John C. Stennis Space Center Education Office. Stennis personnel participated in the final weekend of the Oct. 10-24 festival with education activities and to present information on its new Spaced Out Sports Design Challenge.

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-22

Children react as a tiny Mars Rover rolls over their backs at the USA Science and Engineering Festival, Saturday, Oct. 23, 2010, at Freedom Plaza in Washington. NASA, joined with more than 500 science organizations this weekend to inspire the next generation of scientists and engineers during the first national science and engineering festival held in the nation's capital. Photo Credit: (NASA/Paul E. Alers)

USA Science and Engineering Festival

NASA Image and Video Library

2010-10-22

Priniciples of air flow are explained to visitors to the wind tunnel exhibit at the USA Science and Engineering Festival, Saturday, Oct. 23, 2010, at Freedom Plaza in Washington. NASA, joined with more than 500 science organizations this weekend to inspire the next generation of scientists and engineers during the first national science and engineering festival held in the nation's capital. Photo Credit: (NASA/Paul E. Alers)

Characterizing Wildfire Regimes and Risk in the USA

NASA Astrophysics Data System (ADS)

Malamud, B. D.; Millington, J. D.; Perry, G. L.

2004-12-01

Over the last decade, high profile wildfires have resulted in numerous fatalities and loss of infrastructure. Wildfires also have a significant impact on climate and ecosystems, with recent authors emphasizing the need for regional-level examinations of wildfire-regime dynamics and change, and the factors driving them. With implications for hazard management, climate studies, and ecosystem research, there is therefore significant interest in appropriate analysis of historical wildfire databases. Insightful studies using wildfire database statistics exist, but are often hampered by the low spatial and/or temporal resolution of their datasets. In this paper, we use a high-resolution dataset consisting of 88,855 USFS wildfires over the time period 1970--2000, and consider wildfire occurrence across the conterminous USA as a function of ecoregion (land units classified by climate, vegetation, and topography), ignition source (anthropogenic vs. lightning), and decade (1970--1979, 1980--1989, 1990--1999). We find that for the conterminous USA (a) wildfires exhibit robust frequency-area power-law behavior in 17 different ecoregions, (b) normalized power-law exponents may be used to compare the scaling of wildfire burned areas between regions, (c) power-law exponents change systematically from east to west, (d) wildfires in 75% of the conterminous USA (particularly the east) have higher power-law exponents for anthropogenic vs. lightning ignition sources, and (e) recurrence intervals for wildfires of a given burned area or larger for each ecoregion can be assessed, allowing for the classification of wildfire regimes for probabilistic hazard estimation in the same vein as is now used for earthquakes. By examining wildfire statistics in a spatially and temporally explicit manner, we are able to present resultant wildfire regime summary statistics and conclusions, along with a probabilistic hazard assessment of wildfire risk at the ecoregion division level across the

High-speed reacting flow simulation using USA-series codes

NASA Astrophysics Data System (ADS)

Chakravarthy, S. R.; Palaniswamy, S.

In this paper, the finite-rate chemistry (FRC) formulation for the USA-series of codes and three sets of validations are presented. USA-series computational fluid dynamics (CFD) codes are based on Unified Solution Algorithms including explicity and implicit formulations, factorization and relaxation approaches, time marching and space marching methodolgies, etc., in order to be able to solve a very wide class of CDF problems using a single framework. Euler or Navier-Stokes equations are solved using a finite-volume treatment with upwind Total Variation Diminishing discretization for the inviscid terms. Perfect and real gas options are available including equilibrium and nonequilibrium chemistry. This capability has been widely used to study various problems including Space Shuttle exhaust plumes, National Aerospace Plane (NASP) designs, etc. (1) Numerical solutions are presented showing the full range of possible solutions to steady detonation wave problems. (2) Comparison between the solution obtained by the USA code and Generalized Kinetics Analysis Program (GKAP) is shown for supersonic combustion in a duct. (3) Simulation of combustion in a supersonic shear layer is shown to have reasonable agreement with experimental observations.

Babesia microti Infection, Eastern Pennsylvania, USA

PubMed Central

Ender, Peter T.; Smith, Erin M.; Jahre, Jeffrey A.

2013-01-01

Infection with Babesia microti has not been well-described in eastern Pennsylvania, USA, despite the vector of this organism being prevalent. We report 3 cases of babesiosis in eastern Pennsylvania in persons without recent travel outside the region or history of blood transfusions, suggesting emergence of this infection. PMID:23764008

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

An attendee uses virtual reality goggles to view the Sun during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

Attendees walk past an inflatable model of NASA's Space Launch System during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

An attendee guides NASA's Space Launch System in an interactive exhibit during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

A model of NASA's James Webb Space Telescope is seen during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

USA Science and Engineering Festival 2014

NASA Image and Video Library

2014-04-25

A NASA staff member demonstrates how to build a circuit at the USA Science and Engineering festival on April 25, 2014. The festival was open to the public on April 26 and 27, 2014 and was held at the Washington Convention Center in Washington, DC. Photo Credit: (NASA/Aubrey Gemignani)

National Assessment of Tree City USA Participation According to Geography and Socioeconomic Characteristics

EPA Science Inventory

Tree City USA is a national program that recognizes municipal commitment to community forestry. In return for meeting program requirements, Tree City USA participants expect social, economic, and/or environmental benefits. Understandingthe geographic distribution and socioeconomi...

Acculturation, depression and oral health of immigrants in the USA.

PubMed

Luo, Huabin; Hybels, Celia F; Wu, Bei

2017-12-21

The objectives were to describe the oral health status of immigrants in the USA, describe the association between acculturation and oral health by accounting for the effects of depression and to explore the effects of interaction between acculturation and depression on the oral health of immigrants. Data were from the 2011-2012 National Health and Nutrition Examination Survey (NHANES). Oral health status was assessed by both self-rated oral health and clinically diagnosed periodontitis, each coded as a binary outcome. Acculturation was operationalised as length of stay in the USA and speaking English at home. Depression was assessed using the Patient Health Questionnaire-9. Multiple logistic regression models were used to examine the association of acculturation and depression status with oral health. In 2011-2012, 36.6% immigrants reported poor oral health and 53.0% were diagnosed with periodontitis. A length of stay in the USA of 30+ years (adjusted odds ratio [AOR] = 0.43, 95% confidence interval [95% CI]: 0.21-0.89) reduced the odds of having periodontitis in comparison with a length of stay in the USA of fewer than 5 years. Speaking English at home (AOR = 0.64, 95% CI: 0.43-0.96) reduced the odds of having periodontitis compared with speaking other languages. Depression was negatively associated with self-reported good oral health (AOR = 0.43, 95% CI: 0.20-0.92) and positively associated with clinically diagnosed periodontitis (AOR = 1.89, 95% CI: 1.18-3.04). The effects of acculturation did not differ according to depression status. A longer stay in the USA and speaking English at home were associated with less periodontitis among the immigrants. © 2017 FDI World Dental Federation.

USA hCG reference service, 10-year report.

PubMed

Cole, Laurence A; Laidler, Laura L; Muller, Carolyn Y

2010-08-01

The USA hCG Reference Service has been dealing with cases of persistent low levels of hCG and gestational trophoblastic diseases for 10years. Here we present the complete experience. Total hCG in serum and urine was measured using the Siemen's Immulite 1000 assay. Hyperglycosylated hCG, nicked hCG, free ss-subunit and ss-core fragment were measured using microtiterplate assays with antibodies B152, B151, FBT11 and B210, respectively. The USA hCG Reference Service has identified 83 cases of false-positive hCG, 71 cases of aggressive gestational trophoblastic disease (GTD), 52 cases of minimally invasive GTD, 168 cases of quiescent GTD and 22 cases of placenta site trophoblastic tumor (PSTT). In addition, 103 cases of pituitary hCG have been identified, 60 cases of nontrophoblastic tumor, 4 cases of inherited hCG and 2 cases of Munchausen's syndrome. This is 565 cases total. Multiple new methods are described and tested for diagnosing all of these disorders. The USA hCG Reference Service experience shows new methods for detecting multiple hCG-related disorders and recommends new approaches for detecting these hCG-related disorders. 2010 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Comparing perceptions of dental aesthetics in the USA with those in eleven ethnic groups.

PubMed

Cons, N C; Jenny, J

1994-12-01

The Standard DAI is an orthodontic index based on perceptions of dental aesthetics in the USA. The Standard DAI is a regression equation linking perceptions of the social acceptability of dental aesthetics with the objective intraoral measurements of ten occlusal traits. Since the Standard DAI is based on perceptions of dental aesthetics in the USA it can be used without modification only where perceptions of dental aesthetics are similar to those in the USA. This study was designed to determine whether perceptions of dental aesthetics of students in eleven diverse ethnic groups are similar to those of students in the USA. The same 25 stimuli (photographs of dental configurations, a subset of the 200 stimuli used in deriving the Standard DAI equation) were rated for dental aesthetics by Australian, Chinese, German, Japanese, Korean, Latvian, Native American, Singaporean Chinese, Singaporean Indian, Singaporean Malay, and Thai students. Their ratings were compared with the ratings of the same 25 stimuli by students in the USA. Spearman rank-order correlations ranged from 0.84 to 0.94. These correlations are sufficiently high to show that perceptions of dental aesthetics in all eleven ethnic groups are very similar to the perceptions of USA students. Therefore the Standard DAI can be used without modification in all eleven ethnic groups.

Comparing perceptions of dental aesthetics in the USA with those in eleven ethnic groups.

PubMed

Cons, N C; Jenny, J

1994-10-01

The Standard DAI is an orthodontic index based on perceptions of dental aesthetics in the USA. The Standard DAI is a regression equation linking perceptions of the social acceptability of dental aesthetics with the objective intraoral measurements of ten occlusal traits. Since the Standard DAI is based on perceptions of dental aesthetics in the USA it can be used without modification only where perceptions of dental aesthetics are similar to those in the USA. This study was designed to determine whether perceptions of dental aesthetics of students in eleven diverse ethnic groups are similar to those of students in the USA. The same 25 stimuli (photographs of dental configurations, a subset of the 200 stimuli used in deriving the Standard DAI equation) were rated for dental aesthetics by Australian, Chinese, German, Japanese, Korean, Latvian, Native American, Singaporean Chinese, Singaporean Indian, Singaporean Malay, and Thai students. Their ratings were compared with the ratings of the same 25 stimuli by students in the USA. Spearman rank-order correlations ranged from 0.84 to 0.94. These correlations are sufficiently high to show that perceptions of dental aesthetics in all eleven ethnic groups are very similar to the perceptions of USA students. Therefore the Standard DAI can be used without modification in all eleven ethnic groups.

Characteristics of breast cancer in Central China, literature review and comparison with USA.

PubMed

Chen, Chuang; Sun, Si; Yuan, Jing-Ping; Wang, Yao-Huai; Cao, Tian-Ze; Zheng, Hong-Mei; Jiang, Xue-Qing; Gong, Yi-Ping; Tu, Yi; Yao, Feng; Hu, Ming-Bai; Li, Juan-Juan; Sun, Sheng-Rong; Wei, Wen

2016-12-01

This work was to analyze characteristics of breast cancer (BC) in Central China, summarize main characteristics in China and compare with USA. BC main characteristics from four hospitals in Central China from 2002 to 2012 were collected and analyzed. All the single and large-scale clinical reports covering at least ten years were selected and summarized to calculate the BC characteristics of China. BC Characteristics in USA were selected based on the database from Surveillance, Epidemiology, and End Results (SEER) Program. Age distribution in Central China was normal with one age peak at 45-49 years, displaying differences from USA and Chinese American with two age peaks. BC characteristics in Central China displayed distinct features from USA and Chinese American, including significant younger onset age, lower proportion of patients with stage I, lymph node negative, small tumor size and ER positive. A total ten long-term and large-scale clinical reports were selected for BC characteristics of Mainland China analysis. A total of 53,571 BC patients were enrolled from 1995 to 2012. The main characteristics of BC in Mainland China were similar as that in Central China, but were significant different from developed regions of China (Hong Kong and Taiwan), USA and Chinese American. BC characteristics in Central China displayed representative patterns of Mainland China, while showed distinct patterns from Chinese patients in other developed areas and USA. Copyright © 2016 Elsevier Ltd. All rights reserved.

Mn porphyrin-based SOD mimic, MnTnHex-2-PyP(5+), and non-SOD mimic, MnTBAP(3-), suppressed rat spinal cord ischemia/reperfusion injury via NF-κB pathways.

PubMed

Celic, T; Španjol, J; Bobinac, M; Tovmasyan, A; Vukelic, I; Reboucas, J S; Batinic-Haberle, I; Bobinac, D

2014-12-01

Herein we have demonstrated that both superoxide dismutase (SOD) mimic, cationic Mn(III) meso-tetrakis(N-n-hexylpyridinium-2-yl)porphyrin (MnTnHex-2-PyP(5+)), and non-SOD mimic, anionic Mn(III) meso-tetrakis(4-carboxylatophenyl)porphyrin (MnTBAP(3-)), protect against oxidative stress caused by spinal cord ischemia/reperfusion via suppression of nuclear factor kappa B (NF-κB) pro-inflammatory pathways. Earlier reports showed that Mn(III) N-alkylpyridylporphyrins were able to prevent the DNA binding of NF-κB in an aqueous system, whereas MnTBAP(3-) was not. Here, for the first time, in a complex in vivo system-animal model of spinal cord injury-a similar impact of MnTBAP(3-), at a dose identical to that of MnTnHex-2-PyP(5+), was demonstrated in NF-κB downregulation. Rats were treated subcutaneously at 1.5 mg/kg starting at 30 min before ischemia/reperfusion, and then every 12 h afterward for either 48 h or 7 days. The anti-inflammatory effects of both Mn porphyrins (MnPs) were demonstrated in the spinal cord tissue at both 48 h and 7 days. The downregulation of NF-κB, a major pro-inflammatory signaling protein regulating astrocyte activation, was detected and found to correlate well with the suppression of astrogliosis (as glial fibrillary acidic protein) by both MnPs. The markers of oxidative stress, lipid peroxidation and protein carbonyl formation, were significantly reduced by MnPs. The favorable impact of both MnPs on motor neurons (Tarlov score and inclined plane test) was assessed. No major changes in glutathione peroxidase- and SOD-like activities were demonstrated, which implies that none of the MnPs acted as SOD mimic. Increasing amount of data on the reactivity of MnTBAP(3-) with reactive nitrogen species (RNS) (.NO/HNO/ONOO(-)) suggests that RNS/MnTBAP(3-)-driven modification of NF-κB protein cysteines may be involved in its therapeutic effects. This differs from the therapeutic efficacy of MnTnHex-2-PyP(5+) which presumably occurs via reactive

2018 USA Science and Engineering Festival

NASA Image and Video Library

2018-04-06

Attendees view a Moon rock that was returned by the Apollo 17 mission during Sneak Peek Friday at the USA Science and Engineering Festival, Friday, April 6, 2018 at the Walter E. Washington Convention Center in Washington, DC. The festival is open to the public April 7-8. Photo Credit: (NASA/Joel Kowsky)

RadTown USA Neighborhoods | US EPA

EPA Pesticide Factsheets

2016-09-08

Learn about radiation sources and uses in the interactive, virtual community of RadTown USA! Explore radiation sources and uses in homes and schools, medical buildings and laboratories and outdoors. You will also find information about coal-fired power plants and nuclear power plants, power lines and learn about responding to radiation emergencies.