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Sample records for neodymium 146 target

  1. 146 Kepler-Lamost targets fundamental parameters

    NASA Astrophysics Data System (ADS)

    Wu, Yaqian

    2015-08-01

    Accurate stellar fundamental parameters with high precision are important for distinguishing stellar populationand star study.Turn-off stars are in the relatively vital stellar evolution state. Studying turn-off stars can help us to have a more comprehensive understand of the stellar physics.With the help of observation provided by Lamost project, we obtain atmospheric parameters of 146 turn-off stars from LSP3 pipeline. Combined with stellar pulsation data from Kepler, we can get asteroseismic characteristic of stars,such as Δν and νmax.In this paper,we constructed a grid of evolutionary models, with the mass range from 0.8 to 2.5 M⊙ and metallicities Zini = 0.0085, 0.0105, 0.0130, 0.0165, 0.0200, 0.0250, 0.0300, 0.0400 (i.e.[Fe/H] from -0.3 to 0.4dex).All evolutionary tracks were started in the pre-main sequence birth line and ended at the base of Red Giant Branch.Based on the stellar model grid we constructed,as well as Kepler-Lamost observations, we obtained fundamental parameters of 146 around turn-off stars, and found that 112 targets lied in turn-off state or in the Main Sequence,15 targets are subgiant stars and 7 targets have evolved to the red giants stage.Then we use pulsation code(JIG) of Guenther to extract theorical individual frequencies and calculate theorical Δν.Meanwhile we obtained more precise fundamental parameters of these stars.

  2. Neodymium Magnets.

    ERIC Educational Resources Information Center

    Wida, Sam

    1992-01-01

    Uses extremely strong neodymium magnets to demonstrate several principles of physics including electromagnetic induction, Lenz's Law, domain theory, demagnetization, the Curie point, and magnetic flux lines. (MDH)

  3. miR-146a targets Fos expression in human cardiac cells

    PubMed Central

    Palomer, Xavier; Capdevila-Busquets, Eva; Botteri, Gaia; Davidson, Mercy M.; Rodríguez, Cristina; Martínez-González, José; Vidal, Francisco; Barroso, Emma; Chan, Tung O.; Feldman, Arthur M.; Vázquez-Carrera, Manuel

    2015-01-01

    ABSTRACT miR-146a is a microRNA whose transcript levels are induced in the heart upon activation of NF-κB, a transcription factor induced by pro-inflammatory molecules (such as TNF-α) that is strongly related to the pathogenesis of cardiac disorders. The main goal of this study consisted of studying new roles of miR-146a in cardiac pathological processes caused by the pro-inflammatory cytokine TNF-α. Our results demonstrate that miR-146a transcript levels were sharply increased in cardiac ventricular tissue of transgenic mice with specific overexpression of TNF-α in the heart, and also in a cardiomyocyte cell line of human origin (AC16) exposed to TNF-α. Among all the in silico predicted miR-146a target genes, Fos mRNA and protein levels notably decreased after TNF-α treatment or miR-146a overexpression. These changes correlated with a diminution in the DNA-binding activity of AP-1, the Fos-containing transcription factor complex. Interestingly, AP-1 inhibition was accompanied by a reduction in matrix metalloproteinase (MMP)-9 mRNA levels in human cardiac cells. The specific regulation of this MMP by miR-146a was further confirmed at the secretion and enzymatic activity levels, as well as after anti-miR-mediated miR-146a inhibition. The results reported here demonstrate that Fos is a direct target of miR-146a activity and that downregulation of the Fos–AP-1 pathway by miR-146a has the capacity to inhibit MMP-9 activity. Given that MMP-9 is an AP-1 target gene involved in cardiac remodeling, myocardial dysfunction and progression of heart failure, these findings suggest that miR-146a might be a new and promising therapeutic tool for treating cardiac disorders associated with enhanced inflammation in the heart. PMID:26112171

  4. microRNA-146a targets the L1 cell adhesion molecule and suppresses the metastatic potential of gastric cancer.

    PubMed

    Hou, Zhibo; Yin, Haitao; Chen, Cong; Dai, Xinzheng; Li, Xiaolin; Liu, Baorui; Fang, Xuefeng

    2012-09-01

    Recent studies have shown that microRNA-146a (miR-146a) is associated with cancer metastasis. However, the mechanisms underlying this process remain poorly understood. In this study, we aimed to investigate the potential role of miR-146a in gastric cancer metastasis. A wound-healing assay and a Transwell assay were used to investigate the impact of miR-146a on the migratory and invasive abilities of MKN-45 cells in vitro. MKN-45 cells stably expressing miR-146a or the negative control were transplanted into nude mice through the lateral tail vein to explore the effect of miR-146a on tumor metastasis in vivo. A luciferase reporter assay and western blot analysis were used to identify the potential target genes. Our results show that the overexpression of miR-146a inhibits the invasion and metastasis of MKN-45 cells in vitro and in vivo. Furthermore, the L1 cell adhesion molecule (L1CAM) was identified as a novel target of miR‑146a in gastric cancer. Taken together, our results provide evidence that miR-146a suppresses gastric cancer cell invasion and metastasis in vitro and in vivo, which may be in part due to the downregulation of L1CAM. miR-146a may have the therapeutic potential to suppress gastric cancer metastasis. PMID:22711166

  5. CD146-targeted immunoPET and NIRF Imaging of Hepatocellular Carcinoma with a Dual-Labeled Monoclonal Antibody

    PubMed Central

    Hernandez, Reinier; Sun, Haiyan; England, Christopher G.; Valdovinos, Hector F.; Ehlerding, Emily B.; Barnhart, Todd E.; Yang, Yunan; Cai, Weibo

    2016-01-01

    Overexpression of CD146 has been correlated with aggressiveness, recurrence rate, and poor overall survival in hepatocellular carcinoma (HCC) patients. In this study, we set out to develop a CD146-targeting probe for high-contrast noninvasive in vivo positron emission tomography (PET) and near-infrared fluorescence (NIRF) imaging of HCCs. YY146, an anti-CD146 monoclonal antibody, was employed as a targeting molecule to which we conjugated the zwitterionic near-infrared fluorescence (NIRF) dye ZW800-1 and the chelator deferoxamine (Df). This enabled labeling of Df-YY146-ZW800 with 89Zr and its subsequent detection using PET and NIRF imaging, all without compromising antibody binding properties. Two HCC cell lines expressing high (HepG2) and low (Huh7) levels of CD146 were employed to generate subcutaneous (s.c.) and orthotopic xenografts in athymic nude mice. Sequential PET and NIRF imaging performed after intravenous injection of 89Zr-Df-YY146-ZW800 into tumor-bearing mice unveiled prominent and persistent uptake of the tracer in HepG2 tumors that peaked at 31.65 ± 7.15 percentage of injected dose per gram (%ID/g; n=4) 72 h post-injection. Owing to such marked accumulation, tumor delineation was successful by both PET and NIRF, which facilitated the fluorescence image-guided resection of orthotopic HepG2 tumors, despite the relatively high liver background. CD146-negative Huh7 and CD146-blocked HepG2 tumors exhibited significantly lower 89Zr-Df-YY146-ZW800 accretion (6.1 ± 0.5 and 8.1 ± 1.0 %ID/g at 72 h p.i., respectively; n=4), demonstrating the CD146-specificity of the tracer in vivo. Ex vivo biodistribution and immunofluorescent staining corroborated the accuracy of the imaging data and correlated tracer uptake with in situ CD146 expression. Overall, 89Zr-Df-YY146-ZW800 showed excellent properties as a PET/NIRF imaging agent, including high in vivo affinity and specificity for CD146-expressing HCC. CD146-targeted molecular imaging using dual-labeled YY146

  6. CD146-targeted immunoPET and NIRF Imaging of Hepatocellular Carcinoma with a Dual-Labeled Monoclonal Antibody.

    PubMed

    Hernandez, Reinier; Sun, Haiyan; England, Christopher G; Valdovinos, Hector F; Ehlerding, Emily B; Barnhart, Todd E; Yang, Yunan; Cai, Weibo

    2016-01-01

    Overexpression of CD146 has been correlated with aggressiveness, recurrence rate, and poor overall survival in hepatocellular carcinoma (HCC) patients. In this study, we set out to develop a CD146-targeting probe for high-contrast noninvasive in vivo positron emission tomography (PET) and near-infrared fluorescence (NIRF) imaging of HCCs. YY146, an anti-CD146 monoclonal antibody, was employed as a targeting molecule to which we conjugated the zwitterionic near-infrared fluorescence (NIRF) dye ZW800-1 and the chelator deferoxamine (Df). This enabled labeling of Df-YY146-ZW800 with (89)Zr and its subsequent detection using PET and NIRF imaging, all without compromising antibody binding properties. Two HCC cell lines expressing high (HepG2) and low (Huh7) levels of CD146 were employed to generate subcutaneous (s.c.) and orthotopic xenografts in athymic nude mice. Sequential PET and NIRF imaging performed after intravenous injection of (89)Zr-Df-YY146-ZW800 into tumor-bearing mice unveiled prominent and persistent uptake of the tracer in HepG2 tumors that peaked at 31.65 ± 7.15 percentage of injected dose per gram (%ID/g; n=4) 72 h post-injection. Owing to such marked accumulation, tumor delineation was successful by both PET and NIRF, which facilitated the fluorescence image-guided resection of orthotopic HepG2 tumors, despite the relatively high liver background. CD146-negative Huh7 and CD146-blocked HepG2 tumors exhibited significantly lower (89)Zr-Df-YY146-ZW800 accretion (6.1 ± 0.5 and 8.1 ± 1.0 %ID/g at 72 h p.i., respectively; n=4), demonstrating the CD146-specificity of the tracer in vivo. Ex vivo biodistribution and immunofluorescent staining corroborated the accuracy of the imaging data and correlated tracer uptake with in situ CD146 expression. Overall, (89)Zr-Df-YY146-ZW800 showed excellent properties as a PET/NIRF imaging agent, including high in vivo affinity and specificity for CD146-expressing HCC. CD146-targeted molecular imaging using dual

  7. Selective targeting of liver cancer with the endothelial marker CD146

    PubMed Central

    Thomann, Stefan; Longerich, Thomas; Bazhin, Alexandr V.; Mier, Walter; Schemmer, Peter; Ryschich, Eduard

    2014-01-01

    Hepatocellular carcinomas are well-vascularized tumors; the endothelial cells in these tumors have a specific phenotype. Our aim was to develop a new approach for tumor-specific drug delivery with monoclonal antibody targeting of endothelial ligands. CD146, a molecule expressed on the endothelial surface of hepatocellular carcinoma, was identified as a promising candidate for targeting. In the present study, endothelial cells immediately captured circulating anti-CD146 (ME-9F1) antibody, while antibody binding in tumors was significantly higher than in hepatic endothelium. Macroscopically, after intravenous injection, there were no differences in the mean accumulation of anti-CD146 antibody in tumor compared to liver tissue, due to a compensating higher blood vessel density in the liver tissue. Additional blockade of nontumoral epitopes and intra-arterial administration, improved selective antibody capture in the tumor microvasculature and largely prevented antibody distribution in the lung and liver. The potential practical use of this approach was demonstrated by imaging of radionuclide-labeled ME-9F1 antibody, which showed excellent tumor-selective uptake. Our results provide a promising principle for the use of endothelial markers for intratumoral drug delivery. Tumor endothelium–based access might offer new opportunities for the imaging and therapy of hepatocellular carcinoma and other liver malignancies. PMID:25238265

  8. PLZF-Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a.

    PubMed

    Mu, Hailong; Li, Na; Wu, Jiang; Zheng, Liming; Zhai, Yuanxin; Li, Bo; Song, Wencong; Wang, Jinglu; Zhu, Haijing; Li, Guangpeng; Hua, Jinlian

    2016-04-01

    Previous studies have shown that promyelocytic leukemia zinc finger (PLZF), chemokine (C-X-C motif) receptor 4 (CXCR4) and mir146a were associated with the self-renewal of mouse spermatogonial stem cells (SSCs); however, there is little information on their effects on the fate of livestock SSCs. Here, we have identified a regulatory pathway in dairy goat mGSCs, involving PLZF, mir146a and the SDF-1 receptor CXCR4. PLZF overexpression downregulated mir146a and simultaneously upregulated the expression of CXCR4 protein, whereas PLZF knockdown (siPLZF) induced the specifically opposite effects. The in vitro assays demonstrated that PLZF specifically interacts with and suppresses the mir146a promoter, and mir146a targets CXCR4 to impede its translation. The levels of ERK1/2 phosphorylation in the mGSCs overexpressed CXCR4 and PLZF were upregulated, respectively, whereas mir146a expression was decreased and CXCR4 protein was increased. Mir146a overexpression and siPLZF impaired mGSC proliferation and differentiation, however, Mir146a knockdown induced the opposite effects. The effects of PLZF and mir146a were mediated regulation by mir146a and CXCR4, respectively. Overexpression of CXCR4 or addition of CXCL12 in cultures of dairy goat mGSCs resulted in the upregulation of their signaling, and the phosphorylation of ERK1/2 was increased. Collectively, these findings indicate that PLZF is an important transcription factor in the regulation of the expression of CXCR4 to promote dairy goat mGSC proliferation by targeting mir146a. J. Cell. Biochem. 117: 844-852, 2016. © 2015 Wiley Periodicals, Inc. PMID:26365432

  9. MicroRNA-146a inhibits cell migration and invasion by targeting RhoA in breast cancer

    PubMed Central

    LIU, QIN; WANG, WEI; YANG, XIONGFA; ZHAO, DONGXIAO; LI, FANGQIONG; WANG, HAI

    2016-01-01

    MicroRNAs (miRNAs) function as genetic modulators that regulate gene expression and are involved in a wide range of biological roles, including tumor cell migration and invasion. In the present study, we demonstrated that the migration and invasion activity in MDA-MB-231 breast cancer cells could be directly influenced by altering miR-146a expression. The expression of RhoA and miR-146a in the breast cancer cells showed an inverse correlation. Upregulation of miR-146a in the MDA-MB-231 breast cancer cells by transfection of miR-146a mimics resulted in decreased RhoA protein levels. Conversely, downregulation of miR-146a by transfection of miR-146a inhibitor resulted in increased RhoA protein levels. To confirm the fact that RhoA is a potential target of miR-146a, luciferase reporter containing the RhoA 3′ untranslated region (3′UTR) was constructed. The results demonstrated that the luciferase reporter activity was reduced after overexpression of miR-146a. Moreover, the luciferase reporter which was constructed with the RhoA 3′UTR mutant did not show significantly altered luciferase reporter activity. Furthermore, after treatment with the RhoA inhibitor exoenzyme C3 transferase protein, the migratory capacity of the MDA-MB-231 cells was not significantly altered even though the amount of miR-146a was changed. Our results indicate that miR-146a functions as a tumor suppressor in breast cancer cells. Downregulation of the expression of miR-146a increased the migration of MDA-MB-231 cells, due to the upregulation of RhoA expression. PMID:27175941

  10. Identification of CD146 as a marker enriched for tumor-propagating capacity reveals targetable pathways in primary human sarcoma

    PubMed Central

    Voisin, Veronique; Sato, Shingo; Hirata, Makoto; Whetstone, Heather; Han, Ilkyu; Ailles, Laurie; Bader, Gary D.; Wunder, Jay; Alman, Benjamin A.

    2015-01-01

    Tumor-propagating cells (TPCs) are believed to drive cancer initiation, progression and recurrence. These cells are characterized by enhanced tumorigenicity and self-renewal. The ability to identify such cells in primary human sarcomas relies on the dye exclusion ability of tumor side population (SP) cells. Here, we performed a high-throughput cell surface antigen screen and found that CD146 is enriched in the SP population. In vivo serial transplantation assays showed that CD146+ cells are highly tumorigenic, capable of self-renewal and thus enriches for the TPC population. In addition, depletion of SP cells from the CD146+ population show that CD146+ cells and SP cells are a distinct and overlapping TPC populations. Gene expression profiling of CD146+ and SP cells revealed multiple pathways commonly upregulated in both of these populations. Inhibition of one of these upregulated pathways, Notch signaling, significantly reduced tumor growth and self-renewal. Our data demonstrate that CD146 is an effective cell surface marker for enriching TPCs in primary human sarcomas. Targeting differentially activated pathways in TPCs may provide new therapeutic strategies for treating sarcoma. PMID:26517673

  11. MicroRNA-146a expression inhibits the proliferation and promotes the apoptosis of bronchial smooth muscle cells in asthma by directly targeting the epidermal growth factor receptor

    PubMed Central

    Zhang, Yanxia; Xue, Yan; Liu, Yan; Song, Guodong; Lv, Guofeng; Wang, Yongqiang; Wang, Yijiang; Li, Xiang; Yang, Leiying

    2016-01-01

    The present study aimed to determine the expression of microRNA-146a (miR-146a) in the plasma of children with asthma, and to investigate the effect of miR-146a on the proliferation and apoptosis of bronchial smooth muscle cells (BSMCs). Reverse transcription-quantitative polymerase chain reaction was used to determine the expression levels of miR-146a mimics and its inhibitor. A Cell Counting kit-8 assay was performed to examine the proliferation of BSMCs. Caspase-3/7 activity was determined using a Caspase-Glo 3/7 kit. To measure the expression levels of proteins associated with apoptosis, western blotting was performed. The target gene of miR-146a was identified using a dual-luciferase reporter assay. The plasma levels of miR-146a in children with asthma were significantly higher compared with those in healthy children. Enhanced miR-146a expression inhibited the proliferation of BSMCs. BSMC apoptosis was promoted by miR-146a. The mechanism underlying the miR-146a-induced promotion of BSMC apoptosis may be its direct targeting of epidermal growth factor receptor (EGFR), which affects downstream signaling pathways. In conclusion, miR-146a expression in asthma inhibits the proliferation and promotes the apoptosis of BSMCs by direct targeting of EGFR.

  12. MicroRNA-146a and microRNA-146b regulate human dendritic cell apoptosis and cytokine production by targeting TRAF6 and IRAK1 proteins.

    PubMed

    Park, Haein; Huang, Xin; Lu, Changming; Cairo, Mitchell S; Zhou, Xianzheng

    2015-01-30

    We have previously reported 27 differentially expressed microRNAs (miRNAs) during human monocyte differentiation into immature dendritic cells (imDCs) and mature DCs (mDCs). However, their roles in DC differentiation and function remain largely elusive. Here, we report that microRNA (miR)-146a and miR-146b modulate DC apoptosis and cytokine production. Expression of miR-146a and miR-146b was significantly increased upon monocyte differentiation into imDCs and mDCs. Silencing of miR-146a and/or miR-146b in imDCs and mDCs significantly prevented DC apoptosis, whereas overexpressing miR-146a and/or miR-146b increased DC apoptosis. miR-146a and miR-146b expression in imDCs and mDCs was inversely correlated with TRAF6 and IRAK1 expression. Furthermore, siRNA silencing of TRAF6 and/or IRAK1 in imDCs and mDCs enhanced DC apoptosis. By contrast, lentivirus overexpression of TRAF6 and/or IRAK1 promoted DC survival. Moreover, silencing of miR-146a and miR-146b expression had little effect on DC maturation but enhanced IL-12p70, IL-6, and TNF-α production as well as IFN-γ production by IL-12p70-mediated activation of natural killer cells, whereas miR-146a and miR-146b overexpression in mDCs reduced cytokine production. Silencing of miR-146a and miR-146b in DCs also down-regulated NF-κB inhibitor IκBα and increased Bcl-2 expression. Our results identify a new negative feedback mechanism involving the miR-146a/b-TRAF6/IRAK1-NF-κB axis in promoting DC apoptosis. PMID:25505246

  13. Targeting CD146 with a 64Cu-labeled antibody enables in vivo immunoPET imaging of high-grade gliomas

    PubMed Central

    Yang, Yunan; Hernandez, Reinier; Rao, Jun; Yin, Li; Qu, Yazhuo; Wu, Jinrong; England, Christopher G.; Graves, Stephen A.; Lewis, Christina M.; Wang, Pu; Meyerand, Mary E.; Nickles, Robert J.; Bian, Xiu-wu; Cai, Weibo

    2015-01-01

    Given the highly heterogeneous character of brain malignancies and the associated implication for its proper diagnosis and treatment, finding biomarkers that better characterize this disease from a molecular standpoint is imperative. In this study, we evaluated CD146 as a potential molecular target for diagnosis and targeted therapy of glioblastoma multiforme (GBM), the most common and lethal brain malignancy. YY146, an anti-CD146 monoclonal antibody, was generated and radiolabeled for noninvasive positron-emission tomography (PET) imaging of orthotopic GBM models. 64Cu-labeled YY146 preferentially accumulated in the tumors of mice bearing U87MG xenografts, which allowed the acquisition of high-contrast PET images of small tumor nodules (∼2 mm). Additionally, we found that tumor uptake correlated with the levels of CD146 expression in a highly specific manner. We also explored the potential therapeutic effects of YY146 on the cancer stem cell (CSC) and epithelial-to-mesenchymal (EMT) properties of U87MG cells, demonstrating that YY146 can mitigate those aggressive phenotypes. Using YY146 as the primary antibody, we performed histological studies of World Health Organization (WHO) grades I through IV primary gliomas. The positive correlation found between CD146-positive staining and high tumor grade (χ2 = 9.028; P = 0.029) concurred with the GBM data available in The Cancer Genome Atlas (TCGA) and validated the clinical value of YY146. In addition, we demonstrate that YY146 can be used to detect CD146 in various cancer cell lines and human resected tumor tissues of multiple other tumor types (gastric, ovarian, liver, and lung), indicating a broad applicability of YY146 in solid tumors. PMID:26553993

  14. Targeting CD146 with a 64Cu-labeled antibody enables in vivo immunoPET imaging of high-grade gliomas.

    PubMed

    Yang, Yunan; Hernandez, Reinier; Rao, Jun; Yin, Li; Qu, Yazhuo; Wu, Jinrong; England, Christopher G; Graves, Stephen A; Lewis, Christina M; Wang, Pu; Meyerand, Mary E; Nickles, Robert J; Bian, Xiu-Wu; Cai, Weibo

    2015-11-24

    Given the highly heterogeneous character of brain malignancies and the associated implication for its proper diagnosis and treatment, finding biomarkers that better characterize this disease from a molecular standpoint is imperative. In this study, we evaluated CD146 as a potential molecular target for diagnosis and targeted therapy of glioblastoma multiforme (GBM), the most common and lethal brain malignancy. YY146, an anti-CD146 monoclonal antibody, was generated and radiolabeled for noninvasive positron-emission tomography (PET) imaging of orthotopic GBM models. (64)Cu-labeled YY146 preferentially accumulated in the tumors of mice bearing U87MG xenografts, which allowed the acquisition of high-contrast PET images of small tumor nodules (∼ 2 mm). Additionally, we found that tumor uptake correlated with the levels of CD146 expression in a highly specific manner. We also explored the potential therapeutic effects of YY146 on the cancer stem cell (CSC) and epithelial-to-mesenchymal (EMT) properties of U87MG cells, demonstrating that YY146 can mitigate those aggressive phenotypes. Using YY146 as the primary antibody, we performed histological studies of World Health Organization (WHO) grades I through IV primary gliomas. The positive correlation found between CD146-positive staining and high tumor grade (χ(2) = 9.028; P = 0.029) concurred with the GBM data available in The Cancer Genome Atlas (TCGA) and validated the clinical value of YY146. In addition, we demonstrate that YY146 can be used to detect CD146 in various cancer cell lines and human resected tumor tissues of multiple other tumor types (gastric, ovarian, liver, and lung), indicating a broad applicability of YY146 in solid tumors. PMID:26553993

  15. Using a neodymium magnet to target delivery of ferumoxide-labeled human neural stem cells in a rat model of focal cerebral ischemia.

    PubMed

    Song, Miyeoun; Kim, Young-Ju; Kim, Yoon-ha; Roh, Jina; Kim, Seung U; Yoon, Byung-Woo

    2010-05-01

    Efficiency of targeted delivery of stem cells via transplantation by intravenous injection is limited because of rapid clearance. Thus, more effective, newer methods are required. We hypothesized that combining the use of ferumoxide labeling and magnetic fields could enhance targeted delivery of stem cells. The effects of a magnetic field on proliferation, viability, and differentiation of human neural stem cells (NSCs) were determined in culture, and the results indicated that the difference between control and cultures exposed to a magnetic field were insignificant. To assess migration in vitro, ferumoxide-labeled cells were seeded into a culture dish that had a neodymium magnet below its center, and the labeled NSCs were found to aggregate above the magnet. To investigate targeted delivery of NSCs in vivo, rats were separated into three groups: ischemia only (IO), ischemia with injection of ferumoxide-labeled cells (IC), and ischemia with injection of labeled cells and magnet exposure (ICM). Twenty-four hours after middle cerebral artery occlusion (MCAo), labeled human NSCs were injected into the tail vein. Seven days after MCAo, ICM rats had a larger number and greater distribution of Prussian blue-positive NSCs as compared with controls. In addition, infarct volume in ICM rats was significantly reduced. Our study suggests that this use of a magnetic field may be useful for improving the efficacy of targeted migration of stem cells in stem-based cell therapy in ischemic brain injury. PMID:20059319

  16. Attenuation of cardiac dysfunction in polymicrobial sepsis by microRNA-146a is mediated via targeting of IRAK1 and TRAF6 expression

    PubMed Central

    Gao, Ming; Wang, Xiaohui; Zhang, Xia; Ha, Tuanzhu; Ma, He; Liu, Li; Kalbfleisch, John H.; Gao, Xiang; Kao, Race L.; Williams, David L.; Li, Chuanfu

    2015-01-01

    Cardiac dysfunction is a major consequence of sepsis/septic shock and contributes to the high mortality of sepsis. Innate and inflammatory responses mediated by Toll-like receptors (TLRs) play a critical role in sepsis-induced cardiac dysfunction. MicroRNA-146 was first identified as a negative regulator in innate immune and inflammatory responses induced by LPS. This study examined whether miR-146a will have a protective effect on sepsis-induced cardiac dysfunction. Lentivirus expressing miR-146a (LmiR-146a) or lentivirus expressing scrambled miR (LmiR-control) were delivered into the myocardium via the right carotid artery. Seven days after transfection, mice were subjected to CLP. Untransfected mice were also subjected to CLP-induced sepsis. Cardiac function was examined by echocardiography before and 6 h after CLP. In vitro studies showed that increased miR-146a levels suppresses LPS-induced IκBα phosphorylation and inflammatory cytokine production in both H9C2 cardiomyocytes and J774 macrophages. In vivo transfection of LmiR-146a attenuated sepsis-induced cardiac dysfunction. The values for EF% and FS% in LmiR-146a transfected CLP mice were significantly greater than in untransfected CLP control. LmiR-146a transfection prevented sepsis-induced NF-κB activity, suppressed IRAK and TRAF6 expression in the myocardium, and attenuated sepsis-induced inflammatory cytokine production in both plasma and peritoneal fluid. In addition, LmiR-146a transfection decreased sepsis-induced infiltration of neutrophils and macrophages into the myocardium. LmiR-146a can also transfect macrophages in the periphery. We conclude that miR-146a attenuates sepsis-induced cardiac dysfunction by preventing NF-κB activation, inflammatory cell infiltration, and inflammatory cytokine production via targeting of IRAK and TRAF6 in both cardiomyocytes and inflammatory monocytic cells. PMID:26048146

  17. MicroRNA-146a inhibits epithelial mesenchymal transition in non-small cell lung cancer by targeting insulin receptor substrate 2.

    PubMed

    Park, Dong Ho; Jeon, Hyo Sung; Lee, Soo Young; Choi, Yi Young; Lee, Hae Woo; Yoon, Seongkyu; Lee, Jae Chel; Yoon, Yoo Sang; Kim, Dae Sung; Na, Moon Jun; Kwon, Sun Jung; Kim, Dong Sun; Kang, Jaeku; Park, Jae Yong; Son, Ji Woong

    2015-10-01

    During cancer progression, some tumor cells show changes in their plasticity by morphological and phenotypical conversions, as an expression of mesenchymal markers and loss of epithelial markers, collectively referred to as epithelial-mesenchymal transition (EMT). EMT has been increasingly recognized as a critical phenomenon in lung cancer progression. The goal of this study was to identify microRNAs involved in lung cancer progression. A microarray and qRT-PCR were performed to investigate the miRNA expression profiles in mesenchymal-like lung cancer cells. The role of miR‑146a in lung cancer progression was measured by invasion and migration assays in vitro. Bioinformatics and luciferase report assays were used to identify the target of miR‑146a. The expression of miR‑146a was reduced in mesenchymal-like lung cancer cell lines. The overexpression of miR‑146a induced a marked reduction of the mesenchymal marker and increase the epithelial marker in lung cancer cell lines. Moreover, the overexpression of miR‑146a suppressed lung cancer cell migration and invasion. Co-treatment with miR‑146a and gefitinib treatment showed a significant reduction of invasion in the resistant lung cancer cells induced by EMT. The expression of miR‑146a was downregulated in advanced lung cancer tissues. Insulin receptor substrate 2 (IRS2), an adaptor protein that modulates normal growth, metabolism, survival, and differentiation, was identified as a target of miR‑146a. miR‑146a regulated the expression of IRS2 at the mRNA and protein levels. These data demonstrate for the first time that miR‑146a suppresses lung cancer progression by repressing IRS2 expression. This provides new insight into the post-transcriptional regulation of lung cancer progression by miRNAs, a potential approach for the treatment of lung cancer. PMID:26238771

  18. The regulatory roles of microRNA-146b-5p and its target platelet-derived growth factor receptor α (PDGFRA) in erythropoiesis and megakaryocytopoiesis.

    PubMed

    Zhai, Peng-Fei; Wang, Fang; Su, Rui; Lin, Hai-Shuang; Jiang, Chong-Liang; Yang, Gui-Hua; Yu, Jia; Zhang, Jun-Wu

    2014-08-15

    Emerging evidence has shown that microRNAs have key roles in regulating various normal physiological processes, whereas their deregulated expression is correlated with various diseases. The miR-146 family includes miR-146a and miR-146b, with a distinct expression spectrum in different hematopoietic cells. Recent work indicated that miR-146a has a close relationship with inflammation and autoimmune diseases. miR-146-deficient mice have developed some abnormal hematopoietic phenotypes, suggesting the potential functions of miR-146 in hematopoietic development. In this study, we found that miR-146b was consistently up-regulated in both K562 and CD34(+) hematopoietic stem/progenitor cells (HSPCs) undergoing either erythroid or megakaryocytic differentiation. Remarkably, erythroid and megakaryocytic maturation of K562 cells was induced by excess miR-146b but inhibited by decreased miR-146b levels. More importantly, an mRNA encoding receptor tyrosine kinase, namely platelet-derived growth factor receptor α (PDGFRA), was identified and validated as a direct target of miR-146b in hematopoietic cells. Gain-of-function and loss-of-function assays showed that PDGFRA functioned as a negative regulator in erythroid and megakaryocytic differentiation. miR-146b could ultimately affect the expression of the GATA-1 gene, which is regulated by HEY1 (Hairy/enhancer-of-split related with YRPW motif protein 1), a transcriptional repressor, via inhibition of the PDGFRA/JNK/JUN/HEY1 pathway. Lentivirus-mediated gene transfer also demonstrated that the overexpression of miR-146b promoted erythropoiesis and megakaryocytopoiesis of HSPCs via its regulation on the PDGFRA gene and effects on GATA-1 expression. Moreover, we confirmed that the binding of GATA-1 to the miR-146b promoter and induction of miR-146b during hematopoietic maturation were dependent on GATA-1. Therefore, miR-146b, PDGFRA, and GATA-1 formed a regulatory circuit to promote erythroid and megakaryocytic differentiation

  19. Targeting Smad4 links microRNA-146a to the TGF-beta pathway during retinoid acid induction in acute promyelocytic leukemia cell line.

    PubMed

    Zhong, Hua; Wang, Hai-rong; Yang, Shuang; Zhong, Ji-hua; Wang, Ting; Wang, Chun; Chen, Fang-yuan

    2010-07-01

    The expression pattern of microRNAs (miRNAs) and their potential target genes were investigated in acute promyelocytic leukemia (APL) cell line NB4 cells during all-trans-retinoid acid (ATRA) treatment by using a miRNA microarrays platform and real-time quantitative PCR (RTQ-PCR). MiR-146a as one of the miRNAs down-regulated by ATRA during APL differentiation was identified. Direct interaction between miR146a and its predictive target gene Smad4 were confirmed by Luciferase assay. Down-regulation of miR-146a and upregulation of Smad4 at protein levels were demonstrated. These data suggested that miR-146a might influence proliferation of APL cells through TGF-beta1/Smad signal transduction pathway during ATRA induction. PMID:20577838

  20. Attenuation of Cardiac Dysfunction in Polymicrobial Sepsis by MicroRNA-146a Is Mediated via Targeting of IRAK1 and TRAF6 Expression.

    PubMed

    Gao, Ming; Wang, Xiaohui; Zhang, Xia; Ha, Tuanzhu; Ma, He; Liu, Li; Kalbfleisch, John H; Gao, Xiang; Kao, Race L; Williams, David L; Li, Chuanfu

    2015-07-15

    Cardiac dysfunction is a major consequence of sepsis/septic shock and contributes to the high mortality of sepsis. Innate and inflammatory responses mediated by TLRs play a critical role in sepsis-induced cardiac dysfunction. MicroRNA-146 (miR-146) was first identified as a negative regulator in innate immune and inflammatory responses induced by LPS. This study examined whether miR-146a will have a protective effect on sepsis-induced cardiac dysfunction. Lentivirus-expressing miR-146a (LmiR-146a) or lentivirus-expressing scrambled miR (LmiR-control) was delivered into the myocardium via the right carotid artery. Seven days after transfection, mice were subjected to cecal ligation and puncture (CLP). Untransfected mice were also subjected to CLP-induced sepsis. Cardiac function was examined by echocardiography before and 6 h after CLP. In vitro studies showed that increased miR-146a levels suppress LPS-induced IκBα phosphorylation and inflammatory cytokine production in both H9C2 cardiomyocytes and J774 macrophages. In vivo transfection of LmiR-146a attenuated sepsis-induced cardiac dysfunction. The values for percent ejection fraction and percent fractional shortening in LmiR-146a-transfected CLP mice were significantly greater than in untransfected CLP control. LmiR-146a transfection prevented sepsis-induced NF-κB activity, suppressed IRAK and TRAF6 expression in the myocardium, and attenuated sepsis-induced inflammatory cytokine production in both plasma and peritoneal fluid. In addition, LmiR-146a transfection decreased sepsis-induced infiltration of neutrophils and macrophages into the myocardium. LmiR-146a can also transfect macrophages in the periphery. We conclude that miR-146a attenuates sepsis-induced cardiac dysfunction by preventing NF-κB activation, inflammatory cell infiltration, and inflammatory cytokine production via targeting of IRAK and TRAF6 in both cardiomyocytes and inflammatory monocytic cells. PMID:26048146

  1. MiRNA-146a regulates the maturation and differentiation of vascular smooth muscle cells by targeting NF-κB expression.

    PubMed

    Dong, Shaohong; Xiong, Wei; Yuan, Jianhui; Li, Jianghua; Liu, Jianjun; Xu, Xinyun

    2013-08-01

    Dysfunction of vascular smooth muscle cells (VSMCs) is key in the pathogenesis of proliferative cardiovascular diseases, including atherosclerosis and post-angioplasty restenosis. However, to date, the molecular mechanisms of this pathological process have not been elucidated. Growing evidence indicates that microRNAs (miRNAs) are a class of novel gene regulators. Recently, miR-146a was shown to be highly expressed in rat balloon-injured vascular walls as well as in peripheral blood mononuclear cells (PBMCs) from patients with acute coronary syndrome (ACS). The aim of the present study was to investigate the role of miR-146a in regulating VSMC fate and the possible underlying mechanisms involved. Our results revealed that the expression of miR‑146a was increased in proliferative VSMCs. Subsequently, we observed that the knockdown of miR‑146a significantly inhibited the proliferative and migratory properties of VSMCs in vitro, while it markedly promoted the apoptotic capacity of VSMCs. In addition, we demonstrated that the protein expression of nuclear factor-κBp65 (NF-κBp65) and the proliferative cell nuclear antigen (PCNA), known as critical transcriptional factors, were downregulated. By contrast, the crucial proapoptotic molecule Bax has been revealed to be upregulated following miR‑146a knockdown. These results support the conclusion that miR-146a is a novel regulator of VSMC fate and may be a new biomarker or therapeutic target for proliferative cardiovascular disease. PMID:23784108

  2. Brain endothelial miR-146a negatively modulates T-cell adhesion through repressing multiple targets to inhibit NF-κB activation.

    PubMed

    Wu, Dongsheng; Cerutti, Camilla; Lopez-Ramirez, Miguel A; Pryce, Gareth; King-Robson, Josh; Simpson, Julie E; van der Pol, Susanne Ma; Hirst, Mark C; de Vries, Helga E; Sharrack, Basil; Baker, David; Male, David K; Michael, Gregory J; Romero, Ignacio A

    2015-03-01

    Pro-inflammatory cytokine-induced activation of nuclear factor, NF-κB has an important role in leukocyte adhesion to, and subsequent migration across, brain endothelial cells (BECs), which is crucial for the development of neuroinflammatory disorders such as multiple sclerosis (MS). In contrast, microRNA-146a (miR-146a) has emerged as an anti-inflammatory molecule by inhibiting NF-κB activity in various cell types, but its effect in BECs during neuroinflammation remains to be evaluated. Here, we show that miR-146a was upregulated in microvessels of MS-active lesions and the spinal cord of mice with experimental autoimmune encephalomyelitis. In vitro, TNFα and IFNγ treatment of human cerebral microvascular endothelial cells (hCMEC/D3) led to upregulation of miR-146a. Brain endothelial overexpression of miR-146a diminished, whereas knockdown of miR-146a augmented cytokine-stimulated adhesion of T cells to hCMEC/D3 cells, nuclear translocation of NF-κB, and expression of adhesion molecules in hCMEC/D3 cells. Furthermore, brain endothelial miR-146a modulates NF-κB activity upon cytokine activation through targeting two novel signaling transducers, RhoA and nuclear factor of activated T cells 5, as well as molecules previously identified, IL-1 receptor-associated kinase 1, and TNF receptor-associated factor 6. We propose brain endothelial miR-146a as an endogenous NF-κB inhibitor in BECs associated with decreased leukocyte adhesion during neuroinflammation. PMID:25515214

  3. Brain endothelial miR-146a negatively modulates T-cell adhesion through repressing multiple targets to inhibit NF-κB activation

    PubMed Central

    Wu, Dongsheng; Cerutti, Camilla; Lopez-Ramirez, Miguel A; Pryce, Gareth; King-Robson, Josh; Simpson, Julie E; van der Pol, Susanne MA; Hirst, Mark C; de Vries, Helga E; Sharrack, Basil; Baker, David; Male, David K; Michael, Gregory J; Romero, Ignacio A

    2015-01-01

    Pro-inflammatory cytokine-induced activation of nuclear factor, NF-κB has an important role in leukocyte adhesion to, and subsequent migration across, brain endothelial cells (BECs), which is crucial for the development of neuroinflammatory disorders such as multiple sclerosis (MS). In contrast, microRNA-146a (miR-146a) has emerged as an anti-inflammatory molecule by inhibiting NF-κB activity in various cell types, but its effect in BECs during neuroinflammation remains to be evaluated. Here, we show that miR-146a was upregulated in microvessels of MS-active lesions and the spinal cord of mice with experimental autoimmune encephalomyelitis. In vitro, TNFα and IFNγ treatment of human cerebral microvascular endothelial cells (hCMEC/D3) led to upregulation of miR-146a. Brain endothelial overexpression of miR-146a diminished, whereas knockdown of miR-146a augmented cytokine-stimulated adhesion of T cells to hCMEC/D3 cells, nuclear translocation of NF-κB, and expression of adhesion molecules in hCMEC/D3 cells. Furthermore, brain endothelial miR-146a modulates NF-κB activity upon cytokine activation through targeting two novel signaling transducers, RhoA and nuclear factor of activated T cells 5, as well as molecules previously identified, IL-1 receptor-associated kinase 1, and TNF receptor-associated factor 6. We propose brain endothelial miR-146a as an endogenous NF-κB inhibitor in BECs associated with decreased leukocyte adhesion during neuroinflammation. PMID:25515214

  4. Neodymium-142 evidence for Hadean mafic crust.

    PubMed

    O'Neil, Jonathan; Carlson, Richard W; Francis, Don; Stevenson, Ross K

    2008-09-26

    Neodymium-142 data for rocks from the Nuvvuagittuq greenstone belt in northern Quebec, Canada, show that some rock types have lower 142Nd/144Nd ratios than the terrestrial standard (epsilon142Nd = -0.07 to -0.15). Within a mafic amphibolite unit, 142Nd/144Nd ratios correlate positively with Sm/Nd ratios and produce a 146Sm-142Nd isochron with an age of 4280(-81)(+53) million years. These rocks thus sample incompatible-element-enriched material formed shortly after Earth formation and may represent the oldest preserved crustal section on Earth. PMID:18818357

  5. MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a

    PubMed Central

    Du, Jinghua; Niu, Xuemin; Wang, Yang; Kong, Lingbo; Wang, Rongqi; Zhang, Yuguo; Zhao, Suxian; Nan, Yuemin

    2015-01-01

    Nonalcoholic fibrosing steatohepatitis is a uniform process throughout nonalcoholic fatty liver disease (NAFLD). MicroRNAs (miRNAs) have been suggested to modulate cellular processes in liver diseases. However, the functional role of miRNAs in nonalcoholic fibrosing steatohepatitis is largely unclear. In this study, we systematically analyzed the hepatic miRNAs by microarray analysis in nonalcoholic fibrosing steatohepatitis in C57BL/6J mice induced by methionine-choline deficient (MCD) diet. We identified 19 up-regulated and 18 down-regulated miRNAs in liver with fibrosis. Among these dysregulated miRNAs, miR-146a-5p was the most significant down-regulated miRNA. Luciferase activity assay confirmed that Wnt1 and Wnt5a were both the target genes of miR-146a-5p. Hepatic miR-146a-5p was down-regulated in fibrosing steatohepatitis, but its target genes Wnt1 and Wnt5a and their consequent effectors α-SMA and Col-1 were significantly up-regulated. In addition, miR-146a-5p was downregulated, whilst Wnt1 and Wnt5a were up-regulated in the activated primary hepatic stellate cells (HSCs) compared to the quiescent primary HSCs. Overexpression of miR-146a-5p in HSCs inhibited HSC activation and proliferation, which concomitant with the decreased expressions of Wnt1, Wnt5a, α-SMA and Col-1. In conclusion, miR-146a-5p suppresses activation and proliferation of HSCs in the progress of nonalcoholic fibrosing steatohepatitis through targeting Wnt1 and Wnt5a and consequent effectors α-SMA and Col-1. PMID:26537990

  6. Neodymium and strontium isotopic study of Australasian tektites - New constraints on the provenance and age of target materials

    NASA Technical Reports Server (NTRS)

    Blum, Joel D.; Papanastassiou, D. A.; Wasserburg, G. J.; Koeberl, C.

    1992-01-01

    The Nd and Sr isotopic compositions of Australasian tectites (including two flanged Australian tectites, two low-SiO2 Muong Nong-type tectites, and three high-SiO2 Muong Nong-type tectites) and the Nd, Sm, Sr, and Rb concentrations were investigated by isotope-dilution thermal ionization mass spectrometry, and the Sm-Nd and Rb-Sr isotope systematics were used to study the characteristics of the parental material. It is shown that the Nd and Sr isotopic data provide evidence that all Australasian tektites were derived from a single sedimentary formation with a narrow range of stratigraphic ages close to 170 Ma. It is suggested that all of the Australasian tektites were derived from a single impact event and that the australites represent the upper part of a melt sheet ejected at high velocity, whereas the indochinites represent melts formed at a lower level in the target material distributed closer to the area of the impact.

  7. Association of microRNA-146a and its target gene IRAK1 polymorphism with enthesitis related arthritis category of juvenile idiopathic arthritis.

    PubMed

    Singh, Sushma; Rai, Geeta; Aggarwal, Amita

    2014-10-01

    MicroRNAs (miRNAs) are non-coding RNA that modulate the expression of multiple target genes at the post-transcriptional level. Single-nucleotide polymorphisms (SNPs) in pre-miRNAs can alter miRNA expression, and polymorphism in target molecules can affect binding to target mRNA. Studies have shown an association between miR-146a gene polymorphism and autoimmune diseases. A target for miR-146a is IRAK1. We studied the SNPs of miRNA-146 and IRAK1 to see their association with susceptibility to juvenile idiopathic arthritis-enthesitis-related arthritis (JIA-ERA). One hundred and fifty patients with JIA-ERA (ILAR criteria) were included in the study. A total of 216 blood donors (201 male) with a mean age of 30.5 years served as controls. miR-146a (rs2910164) and its target IRAK1 (rs1059703) at exon-12 region and IRAK1 (rs3027898) at 3'UTR polymorphisms were analyzed using PCR-RFLP method. Among 150 patients, 133 were males and the mean age at onset of disease was 11 (4-16) years, mean disease duration was 4.5 (0.3-12) years. Twenty-two had uveitis and 21 had positive family history of spondyloarthropathy, 73 had enthesitis, 75 had inflammatory back pain, and all had arthritis. HLA B27 was present in 116 patients. Genotype frequency of miR-146a gene was in Hardy-Weinberg equilibrium in healthy controls. The genotype frequency for miR-146a was different in controls and patients [GG (51.85 vs. 50.0 %), GC (42.13 vs. 37.29 %) and CC (6.02 vs. 12.71 %), OR = 2.18; 95 % CI 1.02-4.68; p value = 0.0418]. The allele frequencies of IRAK1 (rs1059703) and IRAK1 (rs3027898) in males and genotype frequency in females were similar in controls and patients. The C allele of IRAK1 (rs1059703) was in linkage disequilibrium with T allele of IRAK1 (rs3027898). The CC genotype of the miR-146a rs2910164 polymorphism was significantly associated with the susceptibility to JIA-ERA. PMID:24719227

  8. miR-146a-5p inhibits TNF-α-induced adipogenesis via targeting insulin receptor in primary porcine adipocytes[S

    PubMed Central

    Wu, Di; Xi, Qian-Yun; Cheng, Xiao; Dong, Tao; Zhu, Xiao-Tong; Shu, Gang; Wang, Li-Na; Jiang, Qing-Yan; Zhang, Yong-Liang

    2016-01-01

    TNF-α is a multifunctional cytokine participating in immune disorders, inflammation, and tumor development with regulatory effects on energy metabolism. Our work focused on the function of TNF-α in adipogenesis of primary porcine adipocytes. TNF-α could suppress the insulin receptor (IR) at the mRNA and protein levels. Microarray analysis of TNF-α-treated porcine adipocytes was used to screen out 29 differentially expressed microRNAs (miRNAs), 13 of which were remarkably upregulated and 16 were intensely downregulated. These 29 differentially expressed miRNAs were predicted to mainly participate in the insulin signaling pathway, adipocytokine signaling pathway, and type 2 diabetes mellitus pathway by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. miR-146a-5p, reportedly involved in immunity and cancer relevant processes, was one of the most highly differentially expressed miRNAs after TNF-α treatment. Red Oil O staining and TG assay revealed that miR-146a-5p suppressed adipogenesis. A dual-luciferase reporter and siRNA assay verified that miR-146a-5p targeted IR and could inhibit its protein expression. miR-146a-5p was also validated to be involved in the insulin signaling pathway by reducing tyrosine phosphorylation of insulin receptor substrate-1. Our study provides the first evidence of miR-146a-5p targeting IR, which facilitates future studies related to obesity and diabetes using pig models. PMID:27324794

  9. Targeting miR-146a to Treat Delayed Wound Healing in Human Diabetic Organ-Cultured Corneas

    PubMed Central

    Winkler, Michael A.; Dib, Christian; Ljubimov, Alexander V.; Saghizadeh, Mehrnoosh

    2014-01-01

    Limbal epithelial stem cells (LESC) residing at the corneal periphery are largely responsible for maintaining corneal optical transparency by continuously supplying new corneal epithelial cells, which mature during their radial migration to the central cornea. Diabetes mellitus (DM) affects all the structures of the eye including the cornea. Frequent epithelial erosions, delayed wound healing, and microbial infections are common alterations of the diabetic eye that can result in vision loss. MicroRNAs (miRNAs) are short non-coding oligonucleotides that regulate gene expression by repressing translation. Our purpose was to understand the role of miR-146a in the human limbal versus central corneal epithelial compartment in normal and pathological conditions such as diabetes mellitus. Using quantitative real-time PCR (QPCR) we found miR-146a enrichment in the limbal corneal compartment. This miRNA was also expressed at higher levels in the diabetic vs. normal limbus. Cell migration and wound closure were significantly delayed in normal and diabetic primary limbal epithelial cells (LEC) transfected with miR-146a. Cells treated with miR-146a had decreased levels of phosphorylated (activated) p38 and EGFR, mediators of epithelial wound healing. Conversely, inhibition of miR-146a significantly enhanced cell migration in both normal and diabetic primary LEC and in diabetic organ-cultured corneas by nearly 40% vs. scrambled miRNA control, accompanied by increased phosphorylated signaling intermediates. Transfection of miR-146a in cultured LEC resulted in an increased immunoreactivity for putative LEC markers Frizzled-7 and K15, whereas inhibition of miR-146a decreased their expressions. These data suggest that miR-146a plays a role in LEC maintenance at the corneal periphery, and its expression is downregulated during their migration towards the central cornea and accompanying terminal differentiation. Furthermore, abnormal miR-146a upregulation may be an important

  10. Molecular Characterization of the Escherichia coli Serogroup O117, O126, and O146 O Antigen Gene Clusters and Development of PCR Assays Targeting Serogroup O117-, O126-, and O146-Specific DNA Sequences

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The O antigen gene clusters of Escherichia coli serogroups O117, O126, and O146 were sequenced, and eleven, ten, and eleven open reading frames (ORFs) were identified, respectively. Genes required for O antigen sugar biosynthesis, sugar transfer and sugar processing in these O-antigen gene clusters...

  11. Neodymium-doped glasses for waveguide lasers

    NASA Astrophysics Data System (ADS)

    Church, Kenneth H.; Zanoni, Raymond; Sapak, David L.; Hayden, Joseph S.

    1994-10-01

    We report recent results from our work on the fabrication of neodymium waveguide lasers. Several neodymium doped glasses. APG-1, LG-680, BK 7 and S 3 made by Schott Glass Technologies, Inc. were studied as candidates for use as waveguide lasers. It was found that S 3, a standard ophthalmic glass, had the best ion-exchange properties of any of the glasses studied. A waveguide laser was successfully made using the neodymium doped S 3 glass.

  12. Strontium and neodymium isotopic study of Libyan Desert Glass: Inherited Pan-African age signatures and new evidence for target material

    NASA Astrophysics Data System (ADS)

    Schaaf, P.; Müller-Sohnius, D. M.

    2002-04-01

    Libyan Desert Glass (LDG) is an impact-related, natural glass of still unknown target material. We have determined Rb-Sr and Sm-Nd isotopic ratios from seven LDG samples and five associated sandstones from the LDG strewn field in the Great Sand Sea, western Egypt. Planar deformation features were recently detected in quartz from these sandstones. 87Sr/86Sr ratios and e-Nd values for LDG range between 0.71219 and 0.71344, and between -16.6 and -17.8, respectively, and hence are distinct from the less radiogenic 87Sr/86Sr ratios of 0.70910-0.71053 and e-Nd values from -6.9 to -9.6 for the local sandstones from the LDG strewn field. Previously published isotopic ratios from the Libyan BP and Oasis crater sandstones are generally incompatible with our LDG values. LDG formation undoubtedly occurred at 29 Ma, but neither the Rb-Sr nor the Sm-Nd isotopic system were rehomogenised during the impact event, as we can deduce from Pan-African ages of ~540 Ma determined from the regression lines from a total of 14 LDG samples from this work and the literature. Together with similar Sr and Nd isotopic values for LDG and granitoid rocks from northeast Africa west of the Nile, these findings point to a sandy matrix target material for the LDG derived from a Precambrian crystalline basement, ruling out the Cretaceous sandstones of the former "Nubian Group" as possible precursors for LDG.

  13. Downregulation of microRNA‑146a inhibits ovarian granulosa cell apoptosis by simultaneously targeting interleukin‑1 receptor‑associated kinase and tumor necrosis factor receptor‑associated factor 6.

    PubMed

    Chen, Xi; Xie, Mingxuan; Liu, Da; Shi, Ke

    2015-10-01

    Premature ovarian failure (POF), an ovarian disorder of multifactorial origin, is defined as the occurrence of amenorrhea, hypergonadotropism and hypoestrogenism in females <40 years old. Apoptosis of ovarian granulosa cells is important in POF and understanding the regulatory mechanism underlying ovarian granulosa cell apoptosis may be beneficial for the management of POF. Increasing evidence suggests that microRNAs (miRs) have a regulatory function in oocyte maturation and ovarian follicular development. In the present study, the expression of miR‑146a in plasma and ovarian granulosa cells obtained from patients with POF, its effect on the apoptosis of ovarian granulosa cells and the possible underlying mechanisms were examined. The present study demonstrated that compared with the control groups, the expression of miR‑146a in the plasma and in ovarian granulosa cells of patients with POF was significantly upregulated. Furthermore, it was found that miR‑146a simultaneously targeted interleukin‑1 receptor‑associated kinase (IRAK1) and tumor necrosis factor receptor‑associated factor 6 (TRAF6), which regulated the activity of nuclear factor‑κB and IκBα. In addition, the results demonstrated that inhibition of the caspase cascade by caspase inhibitors attenuated the effects of miR‑146a on ovarian granulosa cell apoptosis. Taken together, these results suggest that miR‑146a has an important promoting effect on the apoptosis of granulosa cells by targeting IRAK1 and TRAF6 via the caspase cascade pathway. These results may be useful for the management of POF. PMID:26151128

  14. Samarium-146 in the early solar system - Evidence from neodymiun in the Allende meteorite

    NASA Technical Reports Server (NTRS)

    Lugmair, G. W.; Shimamura, T.; Lewis, R. S.; Anders, E.

    1983-01-01

    A carbon-chromite fraction from the Allende C3V chondrite shows strikingly large isotopic enrichments of neodymium-142 (0.47 percent) and neodymium-143 (36 percent). Both apparently formed by alpha decay of samarium-146 and samarium-147 (half-lives 1.03 x 10 to the 8th and 1.06 x 10 to the 11th years), but the isotopic enrichment was greatly magnified by recoil of residual nuclei into a carbon film surounding the samarium-bearing grains. These data provide an improved estimate of the original abundance of extinct samarium-146 in the early solar system, Sm-146/Sm-144 = (4.5 + or - 0.5) x 10 to the -3rd, higher than predicted by some models of p-process nucleosynthesis. It may be possible to use this isotopic pair as a chronometer of the early solar system.

  15. Q-Switching in a Neodymium Laser

    ERIC Educational Resources Information Center

    Holgado, Warein; Sola, Inigo J.; Jarque, Enrique Conejero; Jarabo, Sebastian; Roso, Luis

    2012-01-01

    We present a laboratory experiment for advanced undergraduate or graduate laser-related classes to study the performance of a neodymium laser. In the experiment, the student has to build the neodymium laser using an open cavity. After that, the cavity losses are modulated with an optical chopper located inside, so the Q-switching regime is…

  16. MicroRNA-141 and MicroRNA-146b-5p Inhibit the Prometastatic Mesenchymal Characteristics through the RNA-binding Protein AUF1 Targeting the Transcription Factor ZEB1 and the Protein Kinase AKT*

    PubMed Central

    Al-Khalaf, Huda H.; Aboussekhra, Abdelilah

    2014-01-01

    miR-141 and miR-146b-5p are two important tumor suppressor microRNAs, which control several cancer-related genes and processes. In the present report, we have shown that these microRNAs bind specific sites at the 3′-untranslated region (UTR) of the mRNA-binding protein AUF1, leading to its down-regulation. This inverse correlation between the levels of these microRNAs and AUF1 has been identified in various osteosarcoma cell lines. Additionally, we present clear evidence that AUF1 promotes mesenchymal features in osteosarcoma cells and that miR-141 and miR-146b-5p suppress this prometastatic process through AUF1 repression. Indeed, both microRNAs suppressed the invasion/migration and proliferation abilities of osteosarcoma cells through inhibiting the AKT protein kinase in an AUF1-dependent manner. We have also shown that AUF1 binds to and stabilizes the mRNA of the AKT activator phosphoinositide-dependent kinase-1 (PDK1). Furthermore, miR-141 and miR-146b-5p positively regulate the epithelial markers (E-cadherin and Epcam) and repress the mesenchymal markers (N-cadherin, Vimentin, Twist2, and ZEB1). These effects were mediated via the repression of the epithelial-to-mesenchymal inducer ZEB1 through targeting AUF1, which binds the 3′-UTR of the ZEB1 mRNA and reduces its turnover. These results indicate that at least some tumor suppressor functions of miR-141 and miR-146b-5p are mediated through the repression of the oncogenic potentials of AUF1. Therefore, these 3′-UTR-directed post-transcriptional gene expression regulators constitute promising new targets for diagnostic and/or therapeutic interventions. PMID:25261470

  17. Electrolysis of neodymium oxide. Final report for the period August 19, 1991 through February 28, 1997

    SciTech Connect

    Keller, R.; Larimer, K.T.

    1997-05-01

    The objective of this research was to develop an electrolytic process for the continuous and economic production of neodymium alloys from neodymium oxide. The electrolysis of neodymium oxide continued to show promise for implementation as a low-cost process to produce high- quality neodymium or neodymium-iron alloy.

  18. Radio-Purification of Neodymium Chloride

    SciTech Connect

    Hans, S.; Yeh, M.; Cumming, J. B.; Hahn, R. L.

    2011-04-27

    Organometallic liquid scintillator becomes one of the man detection mediums for neutrino experiment. Liquid-liquid extraction is the method of choice for loading metallic ions of interest into the organic solvents at BNL. High purity of all starting materials is essential for the optimization of synthesis. A newly developed 'self-scavenging' technique was applied to purify undesired radioisotopes from the starting metal compound and found to effectively remove thorium and such containments from the neodymium chloride for SNO+.

  19. Quenching of neodymium fluorescence by molecular hydrogen

    SciTech Connect

    Prohaska, J.D.; Machewirth, D.P.; Snitzer, E.

    1995-04-01

    We show that the hydrogen-loading technique used to enhance a fiber`s ultraviolet photosensitivity for writing Bragg gratings can lead to quenching of the lasing ion`s fluorescence. The neodymium fluorescence and radiative lifetimes are measured for the untreated fiber, the hydrogen-loaded fiber, and the postannealed fiber. We show that postannealing can be used to remove the unreacted hydrogen molecules from the fiber laser and restore the radiative lifetime to near that of its original value.

  20. Segmental irradiation of the bladder with neodymium YAG laser irradiation

    SciTech Connect

    McPhee, M.S.; Mador, D.R.; Tulip, J.; Ritchie, B.; Moore, R.; Lakey, W.H.

    1982-11-01

    The Neodymium YAG laser energy source can be readily adapted for cystoscopic use by some simple modifications of existing urologic equipment. Both the fiberoptic resectoscope and a deflecting cystourethroscope have been adapted for this purpose. Fixation of the fiber tip 1 cm. from the target and use of a divergent beam of 36 degrees allows the delivery of standardized dosage to a relatively large bladder tissue volume. Animal experiments involving 35 mongrel dogs established that repetitive overlapping doses of 200 joules ech can successfully treat a large area of bladder resulting in a full thickness bladder wall injury. This technique has been used in 4 high risk patients with infiltrating bladder cancer without adverse sequelae. The ability to reliably produce a full thickness lesion may give this modality a therapeutic advantage over conventional cautery techniques especially for the treatment of residual infiltrative carcinoma.

  1. Immunohistochemical analysis of CD146 expression in canine skin tumours.

    PubMed

    Abou Asa, S; Anwar, Sh; Yanai, T; Sakai, H

    2016-04-01

    CD146, a cell adhesion molecule, is overexpressed in a variety of carcinomas, including melanoma, prostate cancer, epithelial ovarian cancer, and breast cancer. The level of expression is directly correlated with tumour progression and metastatic potential. The most commonly affected organ for both neoplastic and non-neoplastic tumours is the skin. The objective of this study is to investigate the immunohistochemical expression of CD146 in canine skin tumours of epidermal or follicular origin in 53 squamous cell carcinomas (SCCs), 9 squamous papillomas, 7 infundibular keratinizing acanthomas (IKA), 21 trichoepitheliomas, 13 trichoblastomas, and 3 pilomatricomas. Immunohistochemical results showed that SCCs (90.6%), squamous papilloma (33.3%), IKA (85.7%), trichoepithelioma (85.9%), trichoblastoma (30.8%) and pilomatricoma (100%), respectively, were positive for CD146. The significant expression of CD146 in SCCs supports its importance as a useful treatment target. CD146 could also be used in differentiation of trichoepithelioma and trichoblastoma. PMID:26573287

  2. Holmium laser pumped with a neodymium laser

    SciTech Connect

    Bowman, S.R.; Rabinovich, W.S.

    1991-07-30

    This patent describes a solid-state laser device. It comprises a holmium laser having a first host material doped with an amount of holmium ions sufficient to produce an output laser emission at about 3 {mu}m when the holmium ions in the holmium laser are pumped by a pump beam at a wavelength of about 1.1 {mu}m; and neodymium laser pump source means for supplying a pump beam to pump the holmium ions in the holmium laser at a wavelength of about 1.1 {mu}m.

  3. Embedded of neodymium oxide nanoclusters in silica

    NASA Astrophysics Data System (ADS)

    Duhan, S.; Aghamkar, P.; Singh, M.; Kishore, N.

    2007-06-01

    The solgel process successfully prepared Nd IIO 3 / SiO II nanocomposites. After drying in air at 85°C for three days, samples were heat treated, in air, at 750, 950, 1150 and 1250°C. Characterizations were made by, Infrared spectroscopy, X-ray diffraction, and scanning electron microscopy. Mechanisms of neodymium oxide nano clusters formation in the densified silica matrix with respect to thermal treatment are discussed. XRD profiles confirm the crystallinity of the nanoclusters. The size of the nanoclusters was found in the range 15-30nm.

  4. Samarium-neodymium evolution of meteorites

    NASA Technical Reports Server (NTRS)

    Prinzhofer, A.; Papanastassiou, D. A.; Wasserburg, G. J.

    1992-01-01

    The feasibility of using Sm-147 and Sm-146 as chronometers was investigated using Sm-Nd data obtained on three meteorites: Ibitira, Morristown, and Acapulco. The results of isotope analyses demonstrate the presence of in situ decay of short-lived Sm-146 in these meteorites with initial abundances of Sm-146/Sm-144 between 0.009 and 0.007 in different meteorites. Precisely defined Sm-147/Nd-143 internal isochrons were obtained yielding ages equal to 4.46 +/-0.02 AE for Ibitira, 4.47 +/-0.02 AE for Morristown, and 4.60 +/-0.03 AE for Acapulco. However, detailed examination of the coupled Sm-147/Nd-143 and Sm-146/Nd-142 parent-daughter systematics and of initial Nd-143/Nd-144 values indicated the presence of inconsistencies in the data in spite of the precise isochrons, indicating the need for care in the interpretation of the parent-daughter systematics, even when they appear well behaved.

  5. 22 CFR 146.230 - Transition plans.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Transition plans. 146.230 Section 146.230... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 146.230 Transition plans. (a) Submission of plans. An institution to which § 146.225 applies and that is composed of more than...

  6. 22 CFR 146.230 - Transition plans.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Transition plans. 146.230 Section 146.230... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 146.230 Transition plans. (a) Submission of plans. An institution to which § 146.225 applies and that is composed of more than...

  7. 22 CFR 146.230 - Transition plans.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Transition plans. 146.230 Section 146.230... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 146.230 Transition plans. (a) Submission of plans. An institution to which § 146.225 applies and that is composed of more than...

  8. miR-146a promotes the initiation and progression of melanoma by activating Notch signaling

    PubMed Central

    Forloni, Matteo; Dogra, Shaillay Kumar; Dong, Yuying; Conte, Darryl; Ou, Jianhong; Zhu, Lihua Julie; Deng, April; Mahalingam, Meera; Green, Michael R; Wajapeyee, Narendra

    2014-01-01

    Oncogenic mutations in BRAF and NRAS occur in 70% of melanomas. In this study, we identify a microRNA, miR-146a, that is highly upregulated by oncogenic BRAF and NRAS. Expression of miR-146a increases the ability of human melanoma cells to proliferate in culture and form tumors in mice, whereas knockdown of miR-146a has the opposite effects. We show these oncogenic activities are due to miR-146a targeting the NUMB mRNA, a repressor of Notch signaling. Previous studies have shown that pre-miR-146a contains a single nucleotide polymorphism (C>G rs2910164). We find that the ability of pre-miR-146a/G to activate Notch signaling and promote oncogenesis is substantially higher than that of pre-miR-146a/C. Analysis of melanoma cell lines and matched patient samples indicates that during melanoma progression pre-miR-146a/G is enriched relative to pre-miR-146a/C, resulting from a C-to-G somatic mutation in pre-miR-146a/C. Collectively, our results reveal a central role for miR-146a in the initiation and progression of melanoma. DOI: http://dx.doi.org/10.7554/eLife.01460.001 PMID:24550252

  9. 22 CFR 146.200 - Application.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Application. 146.200 Section 146.200 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Coverage § 146.200 Application. Except as provided in §§ 146.205 through 146.235(a), these...

  10. Luminescence from chromium-neodymium-doped lithium niobate

    NASA Astrophysics Data System (ADS)

    Mahpoud, S.; Chamiel, N.; Weiss, A. M.; Rosenbluh, M.; Herman, A.; Shoham, A.; Lipavsky, B.; Rotman, S. R.

    1999-10-01

    Luminescence from chromium-neodymium-doped lithium niobate (LiNbO 3) was experimentally measured to determine the degree of non-radiative energy transfer between chromium and neodymium ions. Evidence is presented for two different time constants for emission from chromium ions in the material, indicating that non-radiative transfer does occur. Differences between quasi-continuous pumping and pulsed excitation are discussed.

  11. ImmunoPET Imaging of CD146 Expression in Malignant Brain Tumors.

    PubMed

    Hernandez, Reinier; Sun, Haiyan; England, Christopher G; Valdovinos, Hector F; Barnhart, Todd E; Yang, Yunan; Cai, Weibo

    2016-07-01

    Recently, the overexpression of CD146 and its potential as a therapeutic target in high-grade gliomas, the most lethal type of brain cancer, was uncovered. In this study, we describe the generation of (89)Zr-Df-YY146, a novel (89)Zr-labeled monoclonal antibody (mAb) for the targeting and quantification of CD146 expression in a mouse model of glioblastoma, using noninvasive immunoPET imaging. YY146, a high affinity anti-CD146 mAb, was conjugated to deferoxamine (Df) for labeling with the long-lived positron emitter (89)Zr (t1/2: 78.4 h). In vitro assays, including flow cytometry, immunofluorescence microscopy, and Western blot, were performed with two glioblastoma cell lines, U87MG and U251, to determine their CD146 expression levels. Also, YY146 and Df-YY146's CD146-binding affinities were compared using flow cytometry. In vivo CD146-targeting of (89)Zr-Df-YY146 was evaluated by sequential PET imaging, in athymic nude mice bearing subcutaneously implanted U87MG or U251 tumors. CD146 blocking, ex vivo biodistribution, and histological studies were carried out to confirm (89)Zr-Df-YY146 specificity, as well as the accuracy of PET data. In vitro studies exposed elevated CD146 expression levels in U87MG cells, but negligible levels in U251 cells. Flow cytometry revealed no differences in affinity between YY146 and Df-YY146. (89)Zr labeling of Df-YY146 proceeded with excellent yield (∼80%), radiochemical purity (>95%), and specific activity (∼44 GBq/μmol). Longitudinal PET revealed prominent and persistent (89)Zr-Df-YY146 uptake in mice bearing U87MG tumors that peaked at 14.00 ± 3.28%ID/g (n = 4), 48 h post injection of the tracer. Conversely, uptake was significantly lower in CD146-negative U251 tumors (5.15 ± 0.99%ID/g, at 48 h p.i.; n = 4; P < 0.05). Uptake in U87MG tumors was effectively blocked in a competitive inhibition experiment, corroborating the CD146 specificity of (89)Zr-Df-YY146. Finally, ex vivo biodistribution validated the accuracy of PET data

  12. p21 participates in the regulation of anaplastic thyroid cancer cell proliferation by miR-146b

    PubMed Central

    Wang, Shiyang; Chen, Yangjing; Bai, Yanxia

    2016-01-01

    Anaplastic thyroid carcinoma (ATC) originates from completely undifferentiated cells, and is the most lethal type of thyroid-derived tumor. Numerous microRNAs have significant roles in tumorigenesis by targeting relevant genes. The role of microRNA 146b (miR-146b) in ATC remains to be elucidated. In order to characterize the role of miR-146b in ATC, overexpression or interference of miR-146b was induced in ATC cell lines, and cell proliferation and migration were evaluated. The potential targets of miR-146b were searched in the Gene Expression Omnibus database for ATC and matched non-tumor control samples. The expression level of potential targets was detected following overexpression or interference of miR-146b in ATC cell lines. In the present study, cell proliferation was promoted when overexpression of miR-146b was induced in ATC, and inhibited when interference of miR-146b was induced, which indicated that miR-146b affects the proliferation of ATC cells in vitro. In addition, cell migration of ATC was also affected by miR-146b. During the search for potential targets of miR-146b in ATC, p21 (also known as p21Waf1/Cip1 or CDKN1A) was noted for its role in cell cycle progression and tumor pathogenesis. The expression level of p21 was influenced by the level of miR-146b, and the results of the present study demonstrated that the level of p21 was increased when FRO cells were transformed with miR-146b mimic, and p21 was downregulated when FRO cells transformed with anti-miR-146b. In conclusion, p21 may participate in the regulation of ATC cell proliferation by miR-146b. PMID:27602131

  13. Color maps of Arp 146

    NASA Technical Reports Server (NTRS)

    Schultz, A. B.; Spight, L. D.; Colegrove, P. T.; Disanti, M. A.; Fink, U.

    1990-01-01

    Four color maps of Arp 146 are given. The structure and color of the ring galaxy and its companion show evidence of a bridge of material between the companion and the remnant nucleus of the original galaxy now forming the ring. Broad band spatial coverage clearly defines regions of starburst occurrence.

  14. Boundary processes traced by neodymium isotopes

    NASA Astrophysics Data System (ADS)

    Jeandel, C.; Lacan, F.

    2003-04-01

    Continental margins have been identified as preferential sites for removing of reactive elements from the ocean, on the base of U-series measurements (more specifically 231Pa/230Th). This process is called boundary scavenging (Bacon, 1988). Five years of neodymium isotopes data in water masses along the ocean margins (Indonesia, Papua New Guinea, Greenland-Scotland ridge and Labrador Sea) suggests that Nd is transferred from the sediments to the ocean but the reverse also occurs via the so-called boundary scavenging. These processes are only detectable by isotopic ratio measurements because they affect the isotopic signature of the water mass coming in contact with the margin, without changing its concentration. They can involve much higher fluxes than net input processes: for example, the modification of the AAIW signature along the Papua New Guinea slope involves exchange processes only (Lacan and Jeandel, 2001). Since we suspect that such processes not only affect the Nd oceanic chemistry but also the chemical fate of other reactive elements in the ocean, we suggest that the concept of boundary scavenging should be extended to "boundary exchange".

  15. 17 CFR 146.13 - Inspector General exemptions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... (2), (e)(4)(A) through (F), (e)(6), (7), (9), (10), and (11), and (i)) and from 17 CFR 146.3, 146.4...), (d), (e)(1), (e)(4)(G), (H) and (I), and (f) and from 17 CFR 146.3, 146.4, 146.5, 146.6(d), 146.7(a.... 146.13 Section 146.13 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION...

  16. 40 CFR 146.91 - Reporting requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... integrity; or. (5) Pursuant to compliance with the requirement at § 146.90(h) for surface air/soil gas... for formation testing conducted under § 146.82; and (3) Any other planned test of the injection...

  17. 40 CFR 146.91 - Reporting requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... integrity; or. (5) Pursuant to compliance with the requirement at § 146.90(h) for surface air/soil gas... for formation testing conducted under § 146.82; and (3) Any other planned test of the injection...

  18. 40 CFR 146.91 - Reporting requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... integrity; or. (5) Pursuant to compliance with the requirement at § 146.90(h) for surface air/soil gas... for formation testing conducted under § 146.82; and (3) Any other planned test of the injection...

  19. Electrolytic production of neodymium metal from a molten chloride electrolyte

    SciTech Connect

    Chambers, M.F.; Murphy, J.E.

    1991-01-01

    This paper reports that the U.S. Bureau of Mines conducted experiments on electrowinning of neodymium metal by using a molten-metal cathode at 650{degrees} C and an electrolyte of 50 mol pet NdCl, (neodymium chloride) and 50 mol pet KCl (potassium chloride). The molten-metal cathodes were alloys of magnesium and zinc or magnesium and cadmium. Current efficiencies were 90 pct with a Mg-Zn cathode and 80 pct with a Mg-Cd cathode. The Mg-Cd cathode was easily separated from the electrolyte. In contrast, the Mg-Zn cathode tended to mix with the electrolyte, making separation difficult. The cathode metals were separated from the neodymium by distillation at 1,100{degrees} C under a vacuum of 10{sup {minus}4} torr. Neodymium metal of 99.9 + purity was recovered from the Mg-Cd alloy cathode after 30 min distillation time. The neodymium recovered from the Mg-Zn system contained almost 2 pct Zn after vacuum distillation. Continuous operation using the Mg-Cd alloy cathode was demonstrated.

  20. 33 CFR 211.146 - Price.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Price. 211.146 Section 211.146 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE REAL ESTATE... Industrial Facilities § 211.146 Price. No conveyance shall be made for a price less than the fair...

  1. 19 CFR 146.3 - Customs supervision.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Customs supervision. 146.3 Section 146.3 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.3 Customs supervision. (a) Assignment of...

  2. 9 CFR 146.10 - Supervision.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Supervision. 146.10 Section 146.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR COMMERCIAL POULTRY General Provisions § 146.10 Supervision. (a) The Official State Agency...

  3. 19 CFR 146.3 - Customs supervision.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Customs supervision. 146.3 Section 146.3 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.3 Customs supervision. (a) Assignment of...

  4. 19 CFR 146.3 - Customs supervision.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Customs supervision. 146.3 Section 146.3 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.3 Customs supervision. (a) Assignment of...

  5. 19 CFR 146.26 - System review.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false System review. 146.26 Section 146.26 Customs... (CONTINUED) FOREIGN TRADE ZONES Inventory Control and Recordkeeping System § 146.26 System review. The operator shall perform an annual internal review of the inventory control and recordkeeping system...

  6. 19 CFR 146.26 - System review.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false System review. 146.26 Section 146.26 Customs... (CONTINUED) FOREIGN TRADE ZONES Inventory Control and Recordkeeping System § 146.26 System review. The operator shall perform an annual internal review of the inventory control and recordkeeping system...

  7. 19 CFR 146.26 - System review.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false System review. 146.26 Section 146.26 Customs... (CONTINUED) FOREIGN TRADE ZONES Inventory Control and Recordkeeping System § 146.26 System review. The operator shall perform an annual internal review of the inventory control and recordkeeping system...

  8. 9 CFR 146.13 - Testing.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Testing. 146.13 Section 146.13 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR COMMERCIAL POULTRY General Provisions § 146.13 Testing. (a) Samples. Either egg or blood...

  9. 9 CFR 146.13 - Testing.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Testing. 146.13 Section 146.13 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR COMMERCIAL POULTRY General Provisions § 146.13 Testing. (a) Samples. Either egg or blood...

  10. 40 CFR 146.71 - Closure.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Closure. 146.71 Section 146.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class I Hazardous Waste Injection Wells § 146.71 Closure....

  11. 40 CFR 146.71 - Closure.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false Closure. 146.71 Section 146.71 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class I Hazardous Waste Injection Wells § 146.71 Closure....

  12. 22 CFR 146.525 - Fringe benefits.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Fringe benefits. 146.525 Section 146.525... Employment in Education Programs or Activities Prohibited § 146.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical,...

  13. 19 CFR 146.25 - Annual reconciliation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Annual reconciliation. 146.25 Section 146.25 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Inventory Control and Recordkeeping System § 146.25 Annual reconciliation. (a) Report. The operator...

  14. 19 CFR 146.3 - Customs supervision.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Customs supervision. 146.3 Section 146.3 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.3 Customs supervision. (a) Assignment of...

  15. 19 CFR 146.3 - Customs supervision.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Customs supervision. 146.3 Section 146.3 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.3 Customs supervision. (a) Assignment of...

  16. 19 CFR 146.92 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Definitions. 146.92 Section 146.92 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.92 Definitions. (a) Attribution. “Attribution” means the...

  17. 40 CFR 146.51 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Applicability. 146.51 Section 146.51 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class V Injection Wells § 146.51 Applicability....

  18. 33 CFR 146.1 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Applicability. 146.1 Section 146.1 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.1 Applicability. The provisions of this subpart apply to OCS facilities except...

  19. 33 CFR 146.201 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Applicability. 146.201 Section 146.201 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Mobile Offshore Drilling Units § 146.201 Applicability. This subpart applies to mobile offshore...

  20. 33 CFR 146.101 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Applicability. 146.101 Section 146.101 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.101 Applicability. The provisions of this subpart apply only to manned...

  1. 33 CFR 146.200 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Definitions. 146.200 Section 146.200 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Mobile Offshore Drilling Units § 146.200 Definitions. For the purpose of this subpart: Arrives on the OCS...

  2. 33 CFR 146.201 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Applicability. 146.201 Section 146.201 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Mobile Offshore Drilling Units § 146.201 Applicability. This subpart applies to mobile offshore...

  3. 33 CFR 146.101 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Applicability. 146.101 Section 146.101 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.101 Applicability. The provisions of this subpart apply only to manned...

  4. 33 CFR 146.1 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Applicability. 146.1 Section 146.1 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.1 Applicability. The provisions of this subpart apply to OCS facilities except...

  5. 47 CFR 80.146 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 47 Telecommunication 5 2014-10-01 2014-10-01 false 80.146 Section 80.146 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) SAFETY AND SPECIAL RADIO SERVICES STATIONS IN THE MARITIME SERVICES Operating Requirements and Procedures Shipboard General Purpose Watches § 80.146...

  6. 33 CFR 211.146 - Price.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Price. 211.146 Section 211.146 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE REAL ESTATE... Industrial Facilities § 211.146 Price. No conveyance shall be made for a price less than the fair...

  7. 33 CFR 211.146 - Price.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Price. 211.146 Section 211.146 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE REAL ESTATE... Industrial Facilities § 211.146 Price. No conveyance shall be made for a price less than the fair...

  8. 33 CFR 211.146 - Price.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Price. 211.146 Section 211.146 Navigation and Navigable Waters CORPS OF ENGINEERS, DEPARTMENT OF THE ARMY, DEPARTMENT OF DEFENSE REAL ESTATE... Industrial Facilities § 211.146 Price. No conveyance shall be made for a price less than the fair...

  9. 22 CFR 146.510 - Recruitment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Recruitment. 146.510 Section 146.510 Foreign... Education Programs or Activities Prohibited § 146.510 Recruitment. (a) Nondiscriminatory recruitment and hiring. A recipient shall not discriminate on the basis of sex in the recruitment and hiring of...

  10. 7 CFR 1955.146 - Advertising.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 14 2010-01-01 2009-01-01 true Advertising. 1955.146 Section 1955.146 Agriculture... REGULATIONS (CONTINUED) PROPERTY MANAGEMENT Disposal of Inventory Property General § 1955.146 Advertising. (a... real estate brokers, it is the servicing official's responsibility to ensure adequate advertising...

  11. 22 CFR 146.405 - Housing.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Housing. 146.405 Section 146.405 Foreign... Activities Prohibited § 146.405 Housing. (a) Generally. A recipient shall not, on the basis of sex, apply... benefits related to housing, except as provided in this section (including housing provided only to...

  12. 22 CFR 146.540 - Advertising.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Advertising. 146.540 Section 146.540 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR... Education Programs or Activities Prohibited § 146.540 Advertising. A recipient shall not in any...

  13. 7 CFR 1955.146 - Advertising.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 14 2011-01-01 2011-01-01 false Advertising. 1955.146 Section 1955.146 Agriculture... REGULATIONS (CONTINUED) PROPERTY MANAGEMENT Disposal of Inventory Property General § 1955.146 Advertising. (a... real estate brokers, it is the servicing official's responsibility to ensure adequate advertising...

  14. 22 CFR 146.115 - Assurance required.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Assurance required. 146.115 Section 146.115... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 146.115 Assurance required. (a... by, or be covered by a specifically identified assurance from the applicant or...

  15. 22 CFR 146.500 - Employment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Employment. 146.500 Section 146.500 Foreign... ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.500 Employment. (a) General. (1) No person shall, on...

  16. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 28 Judicial Administration 1 2012-07-01 2012-07-01 false Seals. 0.146 Section 0.146 Judicial... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of... the U.S. Marshals Service shall each have custody of the seal pertaining to his...

  17. 10 CFR 72.146 - Design control.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 10 Energy 2 2013-01-01 2013-01-01 false Design control. 72.146 Section 72.146 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE Quality Assurance § 72.146 Design control. (a) The licensee,...

  18. 10 CFR 72.146 - Design control.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Design control. 72.146 Section 72.146 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE Quality Assurance § 72.146 Design control. (a) The licensee,...

  19. 10 CFR 72.146 - Design control.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 10 Energy 2 2011-01-01 2011-01-01 false Design control. 72.146 Section 72.146 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE Quality Assurance § 72.146 Design control. (a) The licensee,...

  20. 10 CFR 72.146 - Design control.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 10 Energy 2 2012-01-01 2012-01-01 false Design control. 72.146 Section 72.146 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE Quality Assurance § 72.146 Design control. (a) The licensee,...

  1. 10 CFR 72.146 - Design control.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 10 Energy 2 2014-01-01 2014-01-01 false Design control. 72.146 Section 72.146 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSING REQUIREMENTS FOR THE INDEPENDENT STORAGE OF SPENT NUCLEAR FUEL, HIGH-LEVEL RADIOACTIVE WASTE, AND REACTOR-RELATED GREATER THAN CLASS C WASTE Quality Assurance § 72.146 Design control. (a) The licensee,...

  2. 19 CFR 146.7 - Zone changes.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Zone changes. 146.7 Section 146.7 Customs Duties U... (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.7 Zone changes. (a) Alteration of an activated area... operations performed in the zone are substantially changed; (3) the existing bond lacks good and...

  3. 19 CFR 146.7 - Zone changes.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Zone changes. 146.7 Section 146.7 Customs Duties U... (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.7 Zone changes. (a) Alteration of an activated area... operations performed in the zone are substantially changed; (3) the existing bond lacks good and...

  4. 19 CFR 146.7 - Zone changes.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Zone changes. 146.7 Section 146.7 Customs Duties U... (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.7 Zone changes. (a) Alteration of an activated area... operations performed in the zone are substantially changed; (3) the existing bond lacks good and...

  5. 19 CFR 146.7 - Zone changes.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Zone changes. 146.7 Section 146.7 Customs Duties U... (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.7 Zone changes. (a) Alteration of an activated area... operations performed in the zone are substantially changed; (3) the existing bond lacks good and...

  6. 40 CFR 146.22 - Construction requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Construction requirements. 146.22 Section 146.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class II Wells § 146.22...

  7. 40 CFR 146.22 - Construction requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Construction requirements. 146.22 Section 146.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class II Wells § 146.22...

  8. 40 CFR 146.12 - Construction requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Construction requirements. 146.12 Section 146.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class I Wells § 146.12...

  9. 40 CFR 146.12 - Construction requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Construction requirements. 146.12 Section 146.12 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class I Wells § 146.12...

  10. 24 CFR 146.37 - Investigation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Investigation. 146.37 Section 146... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.37 Investigation. (a) Investigation and settlement following mediation. (1) HUD shall investigate complaints that are unresolved...

  11. 24 CFR 146.37 - Investigation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Investigation. 146.37 Section 146... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.37 Investigation. (a) Investigation and settlement following mediation. (1) HUD shall investigate complaints that are unresolved...

  12. 22 CFR 146.430 - Financial assistance.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... Education Programs or Activities Prohibited § 146.430 Financial assistance. (a) General. Except as provided... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Financial assistance. 146.430 Section 146.430 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN...

  13. 22 CFR 146.430 - Financial assistance.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Education Programs or Activities Prohibited § 146.430 Financial assistance. (a) General. Except as provided... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Financial assistance. 146.430 Section 146.430 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN...

  14. 21 CFR 133.146 - Grated cheeses.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Grated cheeses. 133.146 Section 133.146 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION CHEESES AND RELATED CHEESE PRODUCTS Requirements for Specific Standardized Cheese and Related Products § 133.146 Grated cheeses....

  15. 22 CFR 146.505 - Employment criteria.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Employment in Education Programs or Activities Prohibited § 146.505 Employment criteria. A recipient shall... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Employment criteria. 146.505 Section 146.505 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN...

  16. 22 CFR 146.430 - Financial assistance.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Financial assistance. 146.430 Section 146.430... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.430 Financial assistance. (a) General. Except as...

  17. 22 CFR 146.430 - Financial assistance.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Financial assistance. 146.430 Section 146.430... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Education Programs or Activities Prohibited § 146.430 Financial assistance. (a) General. Except as...

  18. 22 CFR 146.525 - Fringe benefits.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Fringe benefits. 146.525 Section 146.525... Employment in Education Programs or Activities Prohibited § 146.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical,...

  19. 22 CFR 146.525 - Fringe benefits.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Fringe benefits. 146.525 Section 146.525... Employment in Education Programs or Activities Prohibited § 146.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical,...

  20. 22 CFR 146.525 - Fringe benefits.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Fringe benefits. 146.525 Section 146.525... Employment in Education Programs or Activities Prohibited § 146.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical,...

  1. 22 CFR 146.525 - Fringe benefits.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Fringe benefits. 146.525 Section 146.525... Employment in Education Programs or Activities Prohibited § 146.525 Fringe benefits. (a) “Fringe benefits” defined. For purposes of these Title IX regulations, fringe benefits means: Any medical,...

  2. 24 CFR 146.35 - Mediation.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Mediation. 146.35 Section 146.35... ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.35 Mediation. (a) HUD shall refer to the Federal Mediation and Conciliation Service, a mediation agency designated by the Secretary...

  3. 40 CFR 146.89 - Mechanical integrity.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false Mechanical integrity. 146.89 Section 146.89 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class VI Wells § 146.89 Mechanical integrity....

  4. 40 CFR 146.81 - Applicability.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Applicability. 146.81 Section 146.81 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class VI Wells § 146.81 Applicability. (a) This...

  5. 22 CFR 146.405 - Housing.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Housing. 146.405 Section 146.405 Foreign... Activities Prohibited § 146.405 Housing. (a) Generally. A recipient shall not, on the basis of sex, apply... benefits related to housing, except as provided in this section (including housing provided only to...

  6. microRNA-146a promotes mycobacterial survival in macrophages through suppressing nitric oxide production

    PubMed Central

    Li, Miao; Wang, Jinli; Fang, Yimin; Gong, Sitang; Li, Meiyu; Wu, Minhao; Lai, Xiaomin; Zeng, Gucheng; Wang, Yi; Yang, Kun; Huang, Xi

    2016-01-01

    Macrophages play a crucial role in host innate anti-mycobacterial defense, which is tightly regulated by multiple factors, including microRNAs. Our previous study showed that a panel of microRNAs was markedly up-regulated in macrophages upon mycobacterial infection. Here, we investigated the biological function of miR-146a during mycobacterial infection. miR-146a expression was induced both in vitro and in vivo after Mycobacterium bovis BCG infection. The inducible miR-146a could suppress the inducible nitric oxide (NO) synthase (iNOS) expression and NO generation, thus promoting mycobacterial survival in macrophages. Inhibition of endogenous miR-146a increased NO production and mycobacterial clearance. Moreover, miR-146a attenuated the activation of nuclear factor κB and mitogen-activated protein kinases signaling pathways during BCG infection, which in turn repressed iNOS expression. Mechanistically, miR-146a directly targeted tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) at post-transcriptional level. Silencing TRAF6 decreased iNOS expression and NO production in BCG-infected macrophages, while overexpression of TRAF6 reversed miR-146a-mediated inhibition of NO production and clearance of mycobacteria. Therefore, we demonstrated a novel role of miR-146a in the modulation of host defense against mycobacterial infection by repressing NO production via targeting TRAF6, which may provide a promising therapeutic target for tuberculosis. PMID:27025258

  7. New high-strength neodymium phosphate laser glass

    SciTech Connect

    Galagan, B I; Glushchenko, I N; Denker, B I; Kalachev, Yu L; Mikhailov, Viktor A; Sverchkov, S E; Shcherbakov, Ivan A; Kuleshov, N V

    2009-12-31

    A high-strength neodymium laser glass (SNLG) based on an alumoborophosphate composition is developed and synthesised; its physicochemical, spectral, luminescent, and lasing characteristics are studied. It is found that the chemical stability and thermal resistance of the new glass are considerably higher than the corresponding characteristics of known neodymium-doped phosphate laser glasses. Investigations of lasing upon longitudinal diode pumping showed that, due to the higher thermal resistance, the new glass allows one to obtain output powers twice as high as those of industrial GLS22 glass. (active media)

  8. Nanowatt threshold, alumina sensitized neodymium laser integrated on silicon

    PubMed Central

    Maker, Ashley J.; Armani, Andrea M.

    2013-01-01

    Low threshold lasers based on rare-earth elements have enabled numerous scientific discoveries and innovations in industry. However, pushing the threshold into the sub-microwatt regime has been stymied by a fundamental material phenomenon. Specifically, rare earth dopants form clusters which quench emission and reduce efficiency. Here, we fabricate resonant cavity lasers from neodymium-doped silica films containing alumina. The alumina prevents the clustering of the Neodymium, enabling the lasers to achieve thresholds of 530nanoWatts at room temperature. PMID:24216946

  9. Samarium-neodymium evolution of meteorites

    SciTech Connect

    Prinzhofer, A.; Papanastassiou, D.A.; Wasserburg, G.J. )

    1992-02-01

    The authors have obtained Sm-Nd data on two differentiated meteorites, Ibitira, a eucrite with distinct basaltic texture and with evidence of crystallization; and Morristown, a group 3A mesosiderite; as well as on Acapulco, an unclassified meteorite with chondritic chemical composition and a highly recrystallized texture. They have demonstrated the presence of in situ decay of short-lived {sup 146}Sm in these meteorites with initial abundance of {sup 146}Sm/{sup 144}Sm from 0.009 to 0.007 for the different meteorites. Precisely defined {sup 147}Sm-{sup 143}Nd internal isochrons were obtained yielding ages of 4.60 {+-} 0.03 AE for Acapulco, 4.47 {+-} 0.02 AE for Morristown, and 4.46 {+-} 0.02 AE for Ibitira. The corresponding initial {sup 143}Nd/{sup 144}Nd values obtained were 0.2 {+-} 0.9, 2.1 {+-} 0.7, and 1.6 {+-} 0.8 {var epsilon}u (epsilon units). Detailed consideration of the coupled {sup 147}Sm-{sup 143}Nd and {sup 146}Sm-{sup 142}Nd systematics and of the elevated initial {sup 143}Nd/{sup 144}Nd values indicates the presence of inconsistencies in the data for the two Sm-Nd parent-daughter pairs in spiteof the precise isochrons. The authors present a model which provides an interpretation of the paired Sm-Nd systematics in terms of an impulsive disturbance. The results indicate that three meteorites studied are relatively ancient objects, formed within the first 50 to 100 m.y. of the solar system, by planetary differentiation and impact processes, and were subjected to late metamorphism. The time of late metamorphism is substantially younger than 4.4 AE and possibly as recent as {approximately}3.9 AE. This metamorphism has resulted in partial, very limited reequilibration between plagioclase and phosphate, resulting in the observed shifts in initial {sup 143}Nd/{sup 144}Nd, Sm-Nd model ages, and the {sup 142}Nd/{sup 144}Nd abundances, but preserving the major temporal structures of early original formation and later disturbances.

  10. Neodymium neutron transmission and capture measurements and development of a new transmission detector

    NASA Astrophysics Data System (ADS)

    Barry, Devin P.

    Neodymium is a 235U fission product and is important in reactor neutronic calculations. The aim of this thesis is to improve upon the existing neutron cross section data of neodymium. Neutron capture and transmission measurements were performed by the time-of-flight technique at the Rensselaer Polytechnic Institute LINAC using metallic neodymium samples. The capture measurements were made at the 25-m flight station with a 16-segment Nal multiplicity detector, and the transmission measurements were performed at 15-m and 25-m flight stations, respectively, with 6Li glass scintillation detectors. After the data were collected and reduced, resonance parameters were determined by simultaneously fitting the transmission and capture data with the multilevel R-matrix Bayesian code SAMMY. The resonance parameters for all naturally occurring neodymium isotopes were deduced within the energy range of 1.0 eV to 500 eV. The resulting resonance parameters were used to calculate the capture resonance integral with this energy region and were compared to calculations obtained when using the resonance parameters from ENDF-B/VI. The RPI parameters gave a resonance integral value of 32 +/- 1 barns that is approximately 7% lower than that obtained with the ENDF-B/VI parameters. The current measurements significantly reduce the statistical uncertainties on the resonance parameters when compared with previously published parameters. This thesis also explains the resolution function in detail and discusses its importance when fitting experimental data to extract resonance parameters. More accurate resolution function parameters were determined for epithermal transmission and capture measurements by fitting well known resonances in Uranium-238. Improved transmission bare-bounce target in-beam photomultiplier tube (PMT) resolution function parameters were found and compared to those used previously at the RPI LINAC and a marked improvement in the quality of the fits is shown. In addition

  11. MicroRNA-146a modulates B-cell oncogenesis by regulating Egr1

    PubMed Central

    Contreras, Jorge R.; Palanichamy, Jayanth Kumar; Tran, Tiffany M.; Fernando, Thilini R.; Rodriguez-Malave, Norma I.; Goswami, Neha; Arboleda, Valerie A.; Casero, David; Rao, Dinesh S.

    2015-01-01

    miR-146a is a NF-κB induced microRNA that serves as a feedback regulator of this critical pathway. In mice, deficiency of miR-146a results in hematolymphoid cancer at advanced ages as a consequence of constitutive NF-κB activity. In this study, we queried whether the deficiency of miR-146a contributes to B-cell oncogenesis. Combining miR-146a deficiency with transgenic expression of c-Myc led to the development of highly aggressive B-cell malignancies. Mice transgenic for c-Myc and deficient for miR-146a were characterized by significantly shortened survival, increased lymph node involvement, differential involvement of the spleen and a mature B-cell phenotype. High-throughput sequencing of the tumors revealed significant dysregulation of approximately 250 genes. Amongst these, the transcription factor Egr1 was consistently upregulated in mice deficient for miR-146a. Interestingly, transcriptional targets of Egr1 were enriched in both the high-throughput dataset and in a larger set of miR-146a-deficient tumors. miR-146a overexpression led to downregulation of Egr1 and downstream targets with concomitant decrease in cell growth. Direct targeting of the human EGR1 by miR-146a was seen by luciferase assay. Together our findings illuminate a bona fide role for miR-146a in the modulation of B-cell oncogenesis and reveal the importance of understanding microRNA function in a cell- and disease-specific context. PMID:25906746

  12. Efficient wide-aperture neodymium glass rod amplifiers

    SciTech Connect

    Potemkin, A K; Zhurin, K A; Kirsanov, A V; Kopelovich, E A; Kuznetsov, M V; Kuz'min, A A; Flat, F A; Khazanov, Efim A; Shaikin, A A

    2011-06-30

    Amplifiers based on neodymium phosphate glass rods 60 - 100 mm in diameter are experimentally studied. The amplifiers are pumped by INP-16/250 tubular flash lamps placed in a universal pump cavity with a two-section mirror reflector. A compact high-voltage capacitive energy storage with a preionisation circuit was developed to supply the lamps. (lasers)

  13. Purification of cerium, neodymium and gadolinium for low background experiments

    NASA Astrophysics Data System (ADS)

    Boiko, R. S.; Barabash, A. S.; Belli, P.; Bernabei, R.; Cappella, F.; Cerulli, R.; Danevich, F. A.; Incicchitti, A.; Laubenstein, M.; Mokina, V. M.; Nisi, S.; Poda, D. V.; Polischuk, O. G.; Tretyak, V. I.

    2014-01-01

    Cerium, neodymium and gadolinium contain double beta active isotopes. The most interesting are 150Nd and 160Gd (promising for 0ν2β search), 136Ce (2β+ candidate with one of the highest Q2β). The main problem of compounds containing lanthanide elements is their high radioactive contamination by uranium, radium, actinium and thorium. The new generation 2β experiments require development of methods for a deep purification of lanthanides from the radioactive elements. A combination of physical and chemical methods was applied to purify cerium, neodymium and gadolinium. Liquid-liquid extraction technique was used to remove traces of Th and U from neodymium, gadolinium and for purification of cerium from Th, U, Ra and K. Co-precipitation and recrystallization methods were utilized for further reduction of the impurities. The radioactive contamination of the samples before and after the purification was tested by using ultra-low-background HPGe gamma spectrometry. As a result of the purification procedure the radioactive contamination of gadolinium oxide (a similar purification efficiency was reached also with cerium and neodymium oxides) was decreased from 0.12 Bq/kg to 0.007 Bq/kg in 228Th, from 0.04 Bq/kg to <0.006 Bq/kg in 226Ra, and from 0.9 Bq/kg to 0.04 Bq/kg in 40K. The purification methods are much less efficient for chemically very similar radioactive elements like actinium, lanthanum and lutetium.

  14. Neodymium doped hydroxyapatite theranostic nanoplatforms for colon specific drug delivery applications.

    PubMed

    Victor, Sunita Prem; Paul, Willi; Vineeth, V M; Komeri, Remya; Jayabalan, Muthu; Sharma, Chandra P

    2016-09-01

    Theranostic nanoplatforms integrate therapeutic payloads with diagnostic agents, and help monitor therapeutic response. In this regard, stimuli responsive nanoplatforms further favour combinatorial therapeutic approach that can considerably improve efficacy and specificity of treatment. Herein, we present the engineering of a smart theranostic nanoplatform based on neodymium doped hydroxyapatite (HAN). The presence of neodymium endows the HAN nanoplatforms with near-infrared fluorescence capability. These HAN nanoparticles were then subsequently modified with alginic acid (HANA) to confer pH responsiveness to the synthesized nanoplatforms delivering them to the colon after oral administration. These nanoplatforms possessing optimum size, needle shaped morphology and negative zeta potential, are conducive to cellular internalization. On excitation at 410nm they exhibit near infrared emission at 670nm unraveling their theranostic capabilities. Cytotoxic effects systematically assessed using MTT and live dead assays reveal excellent viability. Raman microscopic imaging technique used to visualize uptake in HeLa cells demonstrate increased uptake from 4 to 16h, with growing cluster size and localization in the cytoplasm. Moreover the concomitant presence of alginic acid manifested advantages of augmented loading and pH dependent release profiles of the model drug, 4 acetyl salicylic acid (4ASA). We could thus establish a theranostic system for early tumour detection, targeted tumour therapy and monitoring of colon cancer that can be administered via the oral route. PMID:27281239

  15. CD146(+) cells are essential for kidney vasculature development.

    PubMed

    Halt, Kimmo J; Pärssinen, Heikki E; Junttila, Sanna M; Saarela, Ulla; Sims-Lucas, Sunder; Koivunen, Peppi; Myllyharju, Johanna; Quaggin, Susan; Skovorodkin, Ilya N; Vainio, Seppo J

    2016-08-01

    The kidney vasculature is critical for renal function, but its developmental assembly mechanisms remain poorly understood and models for studying its assembly dynamics are limited. Here, we tested whether the embryonic kidney contains endothelial cells (ECs) that are heterogeneous with respect to VEGFR2/Flk1/KDR, CD31/PECAM, and CD146/MCAM markers. Tie1Cre;R26R(YFP)-based fate mapping with a time-lapse in embryonic kidney organ culture successfully depicted the dynamics of kidney vasculature development and the correlation of the process with the CD31(+) EC network. Depletion of Tie1(+) or CD31(+) ECs from embryonic kidneys, with either Tie1Cre-induced diphtheria toxin susceptibility or cell surface marker-based sorting in a novel dissociation and reaggregation technology, illustrated substantial EC network regeneration. Depletion of the CD146(+) cells abolished this EC regeneration. Fate mapping of green fluorescent protein (GFP)-marked CD146(+)/CD31(-) cells indicated that they became CD31(+) cells, which took part in EC structures with CD31(+) wild-type ECs. EC network development depends on VEGF signaling, and VEGF and erythropoietin are expressed in the embryonic kidney even in the absence of any external hypoxic stimulus. Thus, the ex vivo embryonic kidney culture models adopted here provided novel ways for targeting renal EC development and demonstrated that CD146(+) cells are critical for kidney vasculature development. PMID:27165833

  16. Role of miR-146a in human chondrocyte apoptosis in response to mechanical pressure injury in vitro

    PubMed Central

    JIN, LEI; ZHAO, JIAN; JING, WENSEN; YAN, SHIJU; WANG, XIN; XIAO, CHUN; MA, BAOAN

    2014-01-01

    MicroRNA (miR)-146a is known to be overexpressed in osteoarthritis (OA). However, the role of miR-146a in OA has not yet been fully elucidated. In the present study, we applied mechanical pressure of 10 MPa to human chondrocytes for 60 min in order to investigate the expression of miR-146a and apoptosis following the mechanical pressure injury. Normal human chondrocytes were transfected with an miR-146a mimic or an inhibitor to regulate miR-146a expression. Potential target genes of miR-146a were predicted using bioinformatics. Moreover, luciferase reporter assay confirmed that Smad4 was a direct target of miR-146a. The expression levels of miR-146a, Smad4 and vascular endothelial growth factor (VEGF) were quantified by quantitative reverse transcription PCR and/or western blot analysis. The effects of miR-146a on apoptosis were detected by Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) flow cytometry. The results indicated that mechanical pressure affected chondrocyte viability and induced the early apoptosis of chondrocytes. Mechanical pressure injury increased the expression levels of miR-146a and VEGF and decreased the levels of Smad4 in the chondrocytes. In the human chondrocytes, the upregulation of miR-146a induced apoptosis, upregulated VEGF expression and downregulated Smad4 expression. In addition, the knockdown of miR-146a reduced cell apoptosis, upregulated Smad4 expression and downregulated VEGF expression. Smad4 was identified as a direct target of miR-146a by harboring a miR-146a binding sequence in the 3′-untranslated region (3′-UTR) of its mRNA. Furthermore, the upregulation of VEGF induced by miR-146a was mediated by Smad4 in the chondrocytes subjected to mechanical pressure injury. These results demonstrated that miR-146a was overexpressed in our chondrocyte model of experimentally induced human mechanical injury, accompanied by the upregulation of VEGF and the downregulation of Smad4 in vitro. Moreover, our data suggest

  17. Role of miR-146a in human chondrocyte apoptosis in response to mechanical pressure injury in vitro.

    PubMed

    Jin, Lei; Zhao, Jian; Jing, Wensen; Yan, Shiju; Wang, Xin; Xiao, Chun; Ma, Baoan

    2014-08-01

    MicroRNA (miR)-146a is known to be overexpressed in osteoarthritis (OA). However, the role of miR-146a in OA has not yet been fully elucidated. In the present study, we applied mechanical pressure of 10 MPa to human chondrocytes for 60 min in order to investigate the expression of miR-146a and apoptosis following the mechanical pressure injury. Normal human chondrocytes were transfected with an miR-146a mimic or an inhibitor to regulate miR-146a expression. Potential target genes of miR-146a were predicted using bioinformatics. Moreover, luciferase reporter assay confirmed that Smad4 was a direct target of miR-146a. The expression levels of miR-146a, Smad4 and vascular endothelial growth factor (VEGF) were quantified by quantitative reverse transcription PCR and/or western blot analysis. The effects of miR-146a on apoptosis were detected by Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) flow cytometry. The results indicated that mechanical pressure affected chondrocyte viability and induced the early apoptosis of chondrocytes. Mechanical pressure injury increased the expression levels of miR-146a and VEGF and decreased the levels of Smad4 in the chondrocytes. In the human chondrocytes, the upregulation of miR-146a induced apoptosis, upregulated VEGF expression and downregulated Smad4 expression. In addition, the knockdown of miR-146a reduced cell apoptosis, upregulated Smad4 expression and downregulated VEGF expression. Smad4 was identified as a direct target of miR-146a by harboring a miR‑146a binding sequence in the 3'-untranslated region (3'-UTR) of its mRNA. Furthermore, the upregulation of VEGF induced by miR‑146a was mediated by Smad4 in the chondrocytes subjected to mechanical pressure injury. These results demonstrated that miR-146a was overexpressed in our chondrocyte model of experimentally induced human mechanical injury, accompanied by the upregulation of VEGF and the downregulation of Smad4 in vitro. Moreover, our data suggest

  18. Materials flow analysis of neodymium, status of rare earth metal in the Republic of Korea.

    PubMed

    Swain, Basudev; Kang, Leeseung; Mishra, Chinmayee; Ahn, JoongWoo; Hong, Hyun Seon

    2015-11-01

    Materials flow analysis of neodymium, status of rare earth elements (REEs) in the Republic of Korea has been investigated. Information from various resources like the Korean Ministry of Environment, Korea international trade association, United Nations Commodity Trade Statistics Database and from individual industry were collected and analyzed for materials flow analysis of neodymium. Demand of neodymium in the Republic of Korea for the year 2010 was 409.5 tons out of which the majority of neodymium, i.e., 68.41% was consumed by domestic electronics industry followed by medical appliances manufacturing (13.36%). The Republic Korea is one of the biggest consumer and leading exporter of these industrial products, absolutely depends on import of neodymium, as the country is lacking natural resources. The Republic of Korea has imported 325.9 tons of neodymium permanent magnet and 79.5 tons of neodymium containing equipment parts mainly for electronics, medical appliances, and heavy/light vehicles manufacturing industry. Out of which 95.4 tons of neodymium permanent magnet get exported as an intermediate product and 140.6 tons of neodymium in the form of consumable products get exported. Worldwide the neodymium is at the high end of supply chain critical metal because of increasing demand, scarcity and irreplaceable for technological application. To bring back the neodymium to supply stream the recycling of end of life neodymium-bearing waste can be a feasible option. Out of total domestic consumption, only 21.9 tons of neodymium have been collected and subsequently recycled. From material flow analysis, the requirement for an efficient recycling system and element-wise material flow management for these REEs in the Republic of Korea were realized and recommended. PMID:26210233

  19. MicroRNA-146a: A Key Regulator of Astrocyte-Mediated Inflammatory Response

    PubMed Central

    Prabowo, Avanita; Fluiter, Kees; Spliet, Wim G. M.; van Rijen, Peter C.; Gorter, Jan A.; Aronica, Eleonora

    2012-01-01

    Increasing evidence supports the involvement of microRNAs (miRNA) in the regulation of inflammation in human neurological disorders. In the present study we investigated the role of miR-146a, a key regulator of the innate immune response, in the modulation of astrocyte-mediated inflammation. Using Taqman PCR and in situ hybridization, we studied the expression of miR-146a in epilepsy-associated glioneuronal lesions which are characterized by prominent activation of the innate immune response. In addition, cultured human astrocytes were used to study the regulation of miR-146a expression in response to proinflammatory cytokines. qPCR and western blot were used to evaluate the effects of overexpression or knockdown of miR-146a on IL-1β signaling. Downstream signaling in the IL-1β pathway, as well as the expression of IL-6 and COX-2 were evaluated by western blot and ELISA. Release several cytokines was evaluated using a human magnetic multiplex cytokine assay on a Luminex® 100™/200™ platform. Increased expression of miR-146a was observed in glioneuronal lesions by Taqman PCR. MiR-146a expression in human glial cell cultures was strongly induced by IL-1β and blocked by IL-1β receptor antagonist. Modulation of miR-146a expression by transfection of astrocytes with anti-miR146a or mimic, regulated the mRNA expression levels of downstream targets of miR-146a (IRAK-1, IRAK-2 and TRAF-6) and the expression of IRAK-1 protein. In addition, the expression of IL-6 and COX-2 upon IL-1β stimulation was suppressed by increased levels of miR-146a and increased by the reduction of miR-146a. Modulation of miR-146a expression affected also the release of several cytokines such as IL-6 and TNF-α. Our observations indicate that in response to inflammatory cues, miR-146a was induced as a negative-feedback regulator of the astrocyte-mediated inflammatory response. This supports an important role of miR-146a in human neurological disorders associated with chronic inflammation

  20. MicroRNA-146a: a key regulator of astrocyte-mediated inflammatory response.

    PubMed

    Iyer, Anand; Zurolo, Emanuele; Prabowo, Avanita; Fluiter, Kees; Spliet, Wim G M; van Rijen, Peter C; Gorter, Jan A; Aronica, Eleonora

    2012-01-01

    Increasing evidence supports the involvement of microRNAs (miRNA) in the regulation of inflammation in human neurological disorders. In the present study we investigated the role of miR-146a, a key regulator of the innate immune response, in the modulation of astrocyte-mediated inflammation. Using Taqman PCR and in situ hybridization, we studied the expression of miR-146a in epilepsy-associated glioneuronal lesions which are characterized by prominent activation of the innate immune response. In addition, cultured human astrocytes were used to study the regulation of miR-146a expression in response to proinflammatory cytokines. qPCR and western blot were used to evaluate the effects of overexpression or knockdown of miR-146a on IL-1β signaling. Downstream signaling in the IL-1β pathway, as well as the expression of IL-6 and COX-2 were evaluated by western blot and ELISA. Release several cytokines was evaluated using a human magnetic multiplex cytokine assay on a Luminex® 100™/200™ platform. Increased expression of miR-146a was observed in glioneuronal lesions by Taqman PCR. MiR-146a expression in human glial cell cultures was strongly induced by IL-1β and blocked by IL-1β receptor antagonist. Modulation of miR-146a expression by transfection of astrocytes with anti-miR146a or mimic, regulated the mRNA expression levels of downstream targets of miR-146a (IRAK-1, IRAK-2 and TRAF-6) and the expression of IRAK-1 protein. In addition, the expression of IL-6 and COX-2 upon IL-1β stimulation was suppressed by increased levels of miR-146a and increased by the reduction of miR-146a. Modulation of miR-146a expression affected also the release of several cytokines such as IL-6 and TNF-α. Our observations indicate that in response to inflammatory cues, miR-146a was induced as a negative-feedback regulator of the astrocyte-mediated inflammatory response. This supports an important role of miR-146a in human neurological disorders associated with chronic inflammation

  1. 33 CFR 146.40 - Diving casualties.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.40 Diving casualties. Diving related casualties are reported in accordance with 46 CFR 197.484 and 197.486. ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Diving casualties. 146.40...

  2. 33 CFR 146.40 - Diving casualties.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.40 Diving casualties. Diving related casualties are reported in accordance with 46 CFR 197.484 and 197.486. ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Diving casualties. 146.40...

  3. 40 CFR 146.8 - Mechanical integrity.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Mechanical integrity. 146.8 Section...) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS General Provisions § 146.8 Mechanical integrity. (a) An injection well has mechanical integrity if: (1) There is no significant leak in the...

  4. 13 CFR 146.105 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Definitions. 146.105 Section 146.105 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW RESTRICTIONS ON LOBBYING General... intent to influence, any communication to or appearance before an officer or employee or any agency,...

  5. 9 CFR 146.21 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Definitions. 146.21 Section 146.21 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... primary purpose of producing eggs for human consumption....

  6. 9 CFR 146.10 - Supervision.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Supervision. 146.10 Section 146.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... authorize qualified persons as State Inspectors to perform the selecting and testing of participating...

  7. 9 CFR 146.11 - Inspections.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Inspections. 146.11 Section 146.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... premises will be conducted if a State Inspector determines that a breach of testing has occurred for...

  8. 9 CFR 146.10 - Supervision.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Supervision. 146.10 Section 146.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... authorize qualified persons as State Inspectors to perform the selecting and testing of participating...

  9. 9 CFR 146.10 - Supervision.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Supervision. 146.10 Section 146.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... authorize qualified persons as State Inspectors to perform the selecting and testing of participating...

  10. 9 CFR 146.11 - Inspections.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Inspections. 146.11 Section 146.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... premises will be conducted if a State Inspector determines that a breach of testing has occurred for...

  11. 9 CFR 146.1 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Definitions. 146.1 Section 146.1 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... shall be construed, respectively, to mean: Administrator. The Administrator, Animal and Plant...

  12. 9 CFR 146.11 - Inspections.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Inspections. 146.11 Section 146.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... premises will be conducted if a State Inspector determines that a breach of testing has occurred for...

  13. 14 CFR 1260.146 - Procurement records.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 14 Aeronautics and Space 5 2010-01-01 2010-01-01 false Procurement records. 1260.146 Section 1260... Higher Education, Hospitals, and Other Non-Profit Organizations Procurement Standards § 1260.146 Procurement records. Procurement records and files for purchases in excess of the small purchase...

  14. 40 CFR 146.61 - Applicability

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Applicability 146.61 Section 146.61 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND... would be sufficient to drive fluids into an underground source of drinking water (USDW). Existing...

  15. 40 CFR 146.61 - Applicability

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Applicability 146.61 Section 146.61 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class I Hazardous Waste Injection Wells §...

  16. 24 CFR 146.27 - Information requirements.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Information requirements. 146.27... AND BUSINESS OPPORTUNITY NONDISCRIMINATION ON THE BASIS OF AGE IN HUD PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Duties of HUD Recipients § 146.27 Information requirements. In order to...

  17. 24 CFR 146.27 - Information requirements.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Information requirements. 146.27... AND BUSINESS OPPORTUNITY NONDISCRIMINATION ON THE BASIS OF AGE IN HUD PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Duties of HUD Recipients § 146.27 Information requirements. In order to...

  18. 24 CFR 146.27 - Information requirements.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Information requirements. 146.27... AND BUSINESS OPPORTUNITY NONDISCRIMINATION ON THE BASIS OF AGE IN HUD PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Duties of HUD Recipients § 146.27 Information requirements. In order to...

  19. 40 CFR 146.8 - Mechanical integrity.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Mechanical integrity. 146.8 Section...) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS General Provisions § 146.8 Mechanical integrity. (a) An injection well has mechanical integrity if: (1) There is no significant leak in the...

  20. 33 CFR 146.40 - Diving casualties.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.40 Diving casualties. Diving related casualties are reported in accordance with 46 CFR 197.484 and 197.486. ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Diving casualties. 146.40...

  1. 33 CFR 146.40 - Diving casualties.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.40 Diving casualties. Diving related casualties are reported in accordance with 46 CFR 197.484 and 197.486. ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Diving casualties. 146.40...

  2. 33 CFR 146.40 - Diving casualties.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.40 Diving casualties. Diving related casualties are reported in accordance with 46 CFR 197.484 and 197.486. ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Diving casualties. 146.40...

  3. 33 CFR 146.45 - Pollution incidents.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Pollution incidents. 146.45...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.45 Pollution incidents. Oil pollution.... Additional provisions concerning liability and compensation because of oil pollution are contained...

  4. 33 CFR 146.45 - Pollution incidents.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Pollution incidents. 146.45...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.45 Pollution incidents. Oil pollution.... Additional provisions concerning liability and compensation because of oil pollution are contained...

  5. 33 CFR 146.45 - Pollution incidents.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Pollution incidents. 146.45...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.45 Pollution incidents. Oil pollution.... Additional provisions concerning liability and compensation because of oil pollution are contained...

  6. 9 CFR 146.11 - Inspections.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Inspections. 146.11 Section 146.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... slaughter. (c) Two or more flocks that are found to be not conforming to protocol in the yearly audit for...

  7. 9 CFR 146.11 - Inspections.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Inspections. 146.11 Section 146.11 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... slaughter. (c) Two or more flocks that are found to be not conforming to protocol in the yearly audit for...

  8. 27 CFR 646.146 - General requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2010-04-01 2010-04-01 false General requirements. 646.146 Section 646.146 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE MISCELLANEOUS REGULATIONS RELATING TO ALCOHOL AND TOBACCO...

  9. 27 CFR 646.146 - General requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 3 2011-04-01 2010-04-01 true General requirements. 646.146 Section 646.146 Alcohol, Tobacco Products, and Firearms BUREAU OF ALCOHOL, TOBACCO, FIREARMS, AND EXPLOSIVES, DEPARTMENT OF JUSTICE MISCELLANEOUS REGULATIONS RELATING TO ALCOHOL AND TOBACCO...

  10. 40 CFR 146.91 - Reporting requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Reporting requirements. 146.91 Section... Wells § 146.91 Reporting requirements. The owner or operator must, at a minimum, provide, as specified... injected over the reporting period and the volume injected cumulatively over the life of the project;...

  11. 7 CFR 1955.146 - Advertising.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 14 2012-01-01 2012-01-01 false Advertising. 1955.146 Section 1955.146 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, RURAL BUSINESS-COOPERATIVE SERVICE, RURAL UTILITIES SERVICE, AND FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE (CONTINUED) PROGRAM REGULATIONS (CONTINUED)...

  12. 9 CFR 146.13 - Testing.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Testing. 146.13 Section 146.13 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK... matrix gene or protein but not to determine hemagglutinin or neuraminidase subtypes. Samples for...

  13. 9 CFR 146.13 - Testing.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Testing. 146.13 Section 146.13 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK... matrix gene or protein but not to determine hemagglutinin or neuraminidase subtypes. Samples for...

  14. 9 CFR 146.13 - Testing.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Testing. 146.13 Section 146.13 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK... matrix gene or protein but not to determine hemagglutinin or neuraminidase subtypes. Samples for...

  15. 9 CFR 146.10 - Supervision.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Supervision. 146.10 Section 146.10 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE... authorize qualified persons as State Inspectors to perform the selecting and testing of participating...

  16. 21 CFR 146.114 - Lemon juice.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Lemon juice. 146.114 Section 146.114 Food and....114 Lemon juice. (a) Identity—(1) Description. Lemon juice is the unfermented juice, obtained by mechanical process, from sound, mature lemons (Citrus limon (L.) Burm. f.), from which seeds...

  17. 21 CFR 146.114 - Lemon juice.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Lemon juice. 146.114 Section 146.114 Food and....114 Lemon juice. (a) Identity—(1) Description. Lemon juice is the unfermented juice, obtained by mechanical process, from sound, mature lemons (Citrus limon (L.) Burm. f.), from which seeds...

  18. 21 CFR 146.114 - Lemon juice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Lemon juice. 146.114 Section 146.114 Food and....114 Lemon juice. (a) Identity—(1) Description. Lemon juice is the unfermented juice, obtained by mechanical process, from sound, mature lemons (Citrus limon (L.) Burm. f.), from which seeds...

  19. 21 CFR 146.114 - Lemon juice.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Lemon juice. 146.114 Section 146.114 Food and....114 Lemon juice. (a) Identity—(1) Description. Lemon juice is the unfermented juice, obtained by mechanical process, from sound, mature lemons (Citrus limon (L.) Burm. f.), from which seeds...

  20. 21 CFR 146.114 - Lemon juice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Lemon juice. 146.114 Section 146.114 Food and....114 Lemon juice. (a) Identity—(1) Description. Lemon juice is the unfermented juice, obtained by mechanical process, from sound, mature lemons (Citrus limon (L.) Burm. f.), from which seeds...

  1. 9 CFR 146.2 - Administration.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 9 Animals and Animal Products 1 2012-01-01 2012-01-01 false Administration. 146.2 Section 146.2... Administration. (a) The Department cooperates through a Memorandum of Understanding with the Official State Agency in the administration of the Plan. In the Memorandum of Understanding, the Official State...

  2. 9 CFR 146.2 - Administration.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Administration. 146.2 Section 146.2... Administration. (a) The Department cooperates through a Memorandum of Understanding with the Official State Agency in the administration of the Plan. In the Memorandum of Understanding, the Official State...

  3. 9 CFR 146.2 - Administration.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 9 Animals and Animal Products 1 2013-01-01 2013-01-01 false Administration. 146.2 Section 146.2... Administration. (a) The Department cooperates through a Memorandum of Understanding with the Official State Agency in the administration of the Plan. In the Memorandum of Understanding, the Official State...

  4. 33 CFR 146.45 - Pollution incidents.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Pollution incidents. 146.45...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.45 Pollution incidents. Oil pollution.... Additional provisions concerning liability and compensation because of oil pollution are contained...

  5. 40 CFR 146.69 - Reporting requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Reporting requirements. 146.69 Section 146.69 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED... description of any event that exceeds operating parameters for annulus pressure or injection pressure...

  6. 22 CFR 146.540 - Advertising.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Advertising. 146.540 Section 146.540 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment...

  7. 21 CFR 146.185 - Pineapple juice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Pineapple juice. 146.185 Section 146.185 Food and....185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct consumption..., ripe pineapple (Ananas comosus L. Merrill). The juice may have been concentrated and...

  8. 21 CFR 146.185 - Pineapple juice.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Pineapple juice. 146.185 Section 146.185 Food and....185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct consumption..., ripe pineapple (Ananas comosus L. Merrill). The juice may have been concentrated and...

  9. 21 CFR 146.185 - Pineapple juice.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Pineapple juice. 146.185 Section 146.185 Food and....185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct consumption..., ripe pineapple (Ananas comosus L. Merrill). The juice may have been concentrated and...

  10. 21 CFR 146.185 - Pineapple juice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Pineapple juice. 146.185 Section 146.185 Food and....185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct consumption..., ripe pineapple (Ananas comosus L. Merrill). The juice may have been concentrated and...

  11. 21 CFR 146.185 - Pineapple juice.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Pineapple juice. 146.185 Section 146.185 Food and....185 Pineapple juice. (a) Identity. (1) Pineapple juice is the juice, intended for direct consumption..., ripe pineapple (Ananas comosus L. Merrill). The juice may have been concentrated and...

  12. 40 CFR 146.85 - Financial responsibility.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 24 2012-07-01 2012-07-01 false Financial responsibility. 146.85... to Class VI Wells § 146.85 Financial responsibility. (a) The owner or operator must demonstrate and maintain financial responsibility as determined by the Director that meets the following conditions:...

  13. 40 CFR 146.85 - Financial responsibility.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false Financial responsibility. 146.85... to Class VI Wells § 146.85 Financial responsibility. (a) The owner or operator must demonstrate and maintain financial responsibility as determined by the Director that meets the following conditions:...

  14. 40 CFR 146.67 - Operating requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Operating requirements. 146.67 Section 146.67 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS Criteria and Standards Applicable to Class I Hazardous Waste Injection Wells...

  15. 33 CFR 146.125 - Emergency drills.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Emergency drills. 146.125 Section... CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.125 Emergency drills. (a) Emergency drills shall be conducted at least once each month by the person in charge of the manned facility. The...

  16. 22 CFR 146.515 - Compensation.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Compensation. 146.515 Section 146.515 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Employment in Education Programs or Activities...

  17. 24 CFR 146.31 - Compliance reviews.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.31 Compliance reviews. (a... 24 Housing and Urban Development 1 2014-04-01 2014-04-01 false Compliance reviews. 146.31 Section... clarification or for correction consistent with this part. (c) HUD may conduct compliance reviews of...

  18. 24 CFR 146.31 - Compliance reviews.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.31 Compliance reviews. (a... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Compliance reviews. 146.31 Section... clarification or for correction consistent with this part. (c) HUD may conduct compliance reviews of...

  19. 24 CFR 146.31 - Compliance reviews.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.31 Compliance reviews. (a... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Compliance reviews. 146.31 Section... clarification or for correction consistent with this part. (c) HUD may conduct compliance reviews of...

  20. 24 CFR 146.31 - Compliance reviews.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.31 Compliance reviews. (a... 24 Housing and Urban Development 1 2012-04-01 2012-04-01 false Compliance reviews. 146.31 Section... clarification or for correction consistent with this part. (c) HUD may conduct compliance reviews of...

  1. 24 CFR 146.31 - Compliance reviews.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... FINANCIAL ASSISTANCE Investigation, Settlement, and Enforcement Procedures § 146.31 Compliance reviews. (a... 24 Housing and Urban Development 1 2013-04-01 2013-04-01 false Compliance reviews. 146.31 Section... clarification or for correction consistent with this part. (c) HUD may conduct compliance reviews of...

  2. 24 CFR 146.27 - Information requirements.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Information requirements. 146.27... AND BUSINESS OPPORTUNITY NONDISCRIMINATION ON THE BASIS OF AGE IN HUD PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Duties of HUD Recipients § 146.27 Information requirements. In order to...

  3. 33 CFR 146.110 - Emergency signals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Emergency signals. 146.110...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.110 Emergency signals. (a) The owner, the owner's agent, or the person in charge shall establish emergency signals to be used...

  4. 33 CFR 146.110 - Emergency signals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Emergency signals. 146.110...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.110 Emergency signals. (a) The owner, the owner's agent, or the person in charge shall establish emergency signals to be used...

  5. 24 CFR 146.27 - Information requirements.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 24 Housing and Urban Development 1 2011-04-01 2011-04-01 false Information requirements. 146.27... FEDERAL FINANCIAL ASSISTANCE Duties of HUD Recipients § 146.27 Information requirements. In order to make..., each recipient shall: (a) Keep records in a form and containing information that HUD determines...

  6. 33 CFR 146.45 - Pollution incidents.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Pollution incidents. 146.45...) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS OCS Facilities § 146.45 Pollution incidents. Oil pollution.... Additional provisions concerning liability and compensation because of oil pollution are contained...

  7. Neodymium-YAG laser vitreolysis in sickle cell retinopathy

    SciTech Connect

    Hrisomalos, N.F.; Jampol, L.M.; Moriarty, B.J.; Serjeant, G.; Acheson, R.; Goldberg, M.F.

    1987-08-01

    Six patients with proliferative sickle cell retinopathy and vitreous bands were treated with the neodymium-YAG (Nd-YAG) laser to accomplish lysis of avascular traction bands or to clear the media in front of the macula. Transection of bands was possible in five of the six cases but in two of these the effect was only partial. Three cases were satisfactorily treated with the Nd-YAG laser application alone, two eventually required conventional vitreoretinal surgery, and one patient's condition stabilized despite failure of the treatment. Complications from the treatment occurred in three cases and included subretinal (choroidal) hemorrhage, preretinal hemorrhage, microperforation of a retinal vein, and focal areas of damage to the retinal pigment epithelium. Neodymium-YAG vitreolysis may be a useful modality in carefully selected patients with proliferative sickle cell retinopathy, but potentially sight-threatening complications may occur.

  8. Treatment of benign urethral strictures using a sapphire tipped neodymium:YAG laser.

    PubMed

    Smith, J A

    1989-11-01

    Sapphire tips increase the energy density and cutting effect of a neodymium:YAG laser. Sapphire tipped neodymium:YAG laser fibers were used to perform urethrotomy in 24 men with benign urethral strictures. The cutting effect was inadequate in 10 patients. Of the 24 patients 16 (67%) had a recurrent stricture within 1 year. Sapphire tipped neodymium:YAG laser fibers offer no apparent advantage over cold knife urethrotomy for treatment of benign urethral strictures. PMID:2810498

  9. Additive-pulse modelocking of non-cw neodymium lasers

    NASA Astrophysics Data System (ADS)

    Heinz, P.; Reuther, A.; Laubereau, A.

    1993-03-01

    Passive modelocking of several flash-lamp pumped neodymium lasers with electro-optic amplitude stabilization is demonstrated using a nonlinear Michelson interferometer. Improved performance is reported for the GSGG- YLF- and glass-laser as compared to the nonlinear absorber, with shorter pulse durations and smaller amplitude fluctuations, e.g. 5 μJ pulses for 460 ± 20 fs for Nd:glass. Evidence is obtained for multi-selfstability of the pulse energy.

  10. [Neodymium magnet injury causing nasal fracture: a case report].

    PubMed

    Aykan, Andaç; Güzey, Serbülent; Avşar, Sedat; Öztürk, Serdar

    2015-05-01

    In parallel with technological developments, small size but strong magnets are commonly used in modern devices. In terms of foreign body injuries, magnet injuries are quite rare. However, due to their unique characteristics, there are some difficulties in their management. The magnetic field generated by the magnet affects the surgical instruments and make treatment difficult. In this case report, a nasal injury due to neodymium magnet and our alternative approach for its management was reported. PMID:26033660

  11. Anti-Inflammatory Role of MicroRNA-146a in the Pathogenesis of Diabetic Nephropathy.

    PubMed

    Bhatt, Kirti; Lanting, Linda L; Jia, Ye; Yadav, Sailee; Reddy, Marpadga A; Magilnick, Nathaniel; Boldin, Mark; Natarajan, Rama

    2016-08-01

    Inflammation has a critical role in the pathogenesis of diabetic complications, including diabetic nephropathy (DN). MicroRNAs have recently emerged as important regulators of DN. However, the role of microRNAs in the regulation of inflammation during DN is poorly understood. Here, we examined the in vivo role of microRNA-146a (miR-146a), a known anti-inflammatory microRNA, in the pathogenesis of DN. In a model of streptozotocin-induced diabetes, miR-146a(-/-) mice showed significantly exacerbated proteinuria, renal macrophage infiltration, glomerular hypertrophy, and fibrosis relative to the respective levels in control wild-type mice. Diabetes-induced upregulation of proinflammatory and profibrotic genes was significantly greater in the kidneys of miR-146a(-/-) than in the kidneys of wild-type mice. Notably, miR-146a expression increased in both peritoneal and intrarenal macrophages in diabetic wild-type mice. Mechanistically, miR-146a deficiency during diabetes led to increased expression of M1 activation markers and suppression of M2 markers in macrophages. Concomitant with increased expression of proinflammatory cytokines, such as IL-1β and IL-18, markers of inflammasome activation also increased in the macrophages of diabetic miR-146a(-/-) mice. These studies suggest that in early DN, miR-146a upregulation exerts a protective effect by downregulating target inflammation-related genes, resulting in suppression of proinflammatory and inflammasome gene activation. Loss of this protective mechanism in miR-146a(-/-) mice leads to accelerated DN. Taken together, these results identify miR-146a as a novel anti-inflammatory noncoding RNA modulator of DN. PMID:26647423

  12. Complexation of di-amides of dipicolinic acid with neodymium

    SciTech Connect

    Lapka, J.L.; Paulenova, A.

    2013-07-01

    Di-amides have undergone significant studies as possible ligands for use in the partitioning of trivalent minor actinides and lanthanides. The binding affinities of three isomeric ligands with neodymium in acetonitrile solution have been investigated. The stability constants of the metal-ligand complexes formed between different isomers of N,N'-diethyl-N,N'- ditolyl-di-picolinamide (EtTDPA) and trivalent neodymium in acetonitrile have been determined by spectrophotometric and calorimetric methods. Each isomer of EtTDPA has been found to be capable of forming three complexes with trivalent neodymium, Nd(EtTDPA), Nd(EtTDPA){sub 2}, and Nd(EtTDPA){sub 3}. Values from spectrophotometric and calorimetric titrations are within reasonable agreement with each other. The order of stability constants for each metal:ligand complex decreases in the order Et(m)TDPA > Et(p)TDPA > Et(o)TDPA. The obtained values are comparable to other di-amidic ligands obtained under similar system conditions and mirror previously obtained solvent extraction data for EtTDPA at low ionic strengths. (authors.

  13. MicroRNA 146 (Mir146) modulates spermatogonial differentiation by retinoic acid in mice.

    PubMed

    Huszar, Jessica M; Payne, Christopher J

    2013-01-01

    Impaired biogenesis of microRNAs disrupts spermatogenesis and leads to infertility in male mice. Spermatogonial differentiation is a key step in spermatogenesis, yet the mechanisms that control this event remain poorly defined. In this study, we discovered microRNA 146 (Mir146) to be highly regulated during spermatogonial differentiation, a process dependent on retinoic acid (RA) signaling. Mir146 transcript levels were diminished nearly 180-fold in differentiating spermatogonia when compared with undifferentiated spermatogonia. Luciferase assays revealed the direct binding of Mir146 to the 3' untranslated region of the mediator complex subunit 1 (Med1), a coregulator of retinoid receptors (RARs and RXRs). Overexpression of Mir146 in cultured undifferentiated spermatogonia reduced Med1 transcript levels, as well as those of differentiation marker kit oncogene (Kit). MED1 protein was also diminished. Conversely, inhibition of Mir146 increased the levels of Kit. When undifferentiated spermatogonia were exposed to RA, Mir146 was downregulated along with a marker for undifferentiated germ cells, zinc finger and BTB domain containing 16 (Zbtb16; Plzf); Kit was upregulated. Overexpression of Mir146 in RA-treated spermatogonia inhibited the upregulation of Kit, stimulated by retinoic acid gene 8 (Stra8), and spermatogenesis- and oogenesis-specific basic helix-loop-helix 2 (Sohlh2). Inhibition of Mir146 in RA-treated spermatogonia greatly enhanced the upregulation of these genes. We conclude that Mir146 modulates the effects of RA on spermatogonial differentiation. PMID:23221399

  14. MicroRNA 146 (Mir146) Modulates Spermatogonial Differentiation by Retinoic Acid in Mice1

    PubMed Central

    Huszar, Jessica M.; Payne, Christopher J.

    2012-01-01

    ABSTRACT Impaired biogenesis of microRNAs disrupts spermatogenesis and leads to infertility in male mice. Spermatogonial differentiation is a key step in spermatogenesis, yet the mechanisms that control this event remain poorly defined. In this study, we discovered microRNA 146 (Mir146) to be highly regulated during spermatogonial differentiation, a process dependent on retinoic acid (RA) signaling. Mir146 transcript levels were diminished nearly 180-fold in differentiating spermatogonia when compared with undifferentiated spermatogonia. Luciferase assays revealed the direct binding of Mir146 to the 3′ untranslated region of the mediator complex subunit 1 (Med1), a coregulator of retinoid receptors (RARs and RXRs). Overexpression of Mir146 in cultured undifferentiated spermatogonia reduced Med1 transcript levels, as well as those of differentiation marker kit oncogene (Kit). MED1 protein was also diminished. Conversely, inhibition of Mir146 increased the levels of Kit. When undifferentiated spermatogonia were exposed to RA, Mir146 was downregulated along with a marker for undifferentiated germ cells, zinc finger and BTB domain containing 16 (Zbtb16; Plzf); Kit was upregulated. Overexpression of Mir146 in RA-treated spermatogonia inhibited the upregulation of Kit, stimulated by retinoic acid gene 8 (Stra8), and spermatogenesis- and oogenesis-specific basic helix-loop-helix 2 (Sohlh2). Inhibition of Mir146 in RA-treated spermatogonia greatly enhanced the upregulation of these genes. We conclude that Mir146 modulates the effects of RA on spermatogonial differentiation. PMID:23221399

  15. The Role of MicroRNA-146a in the Pathogenesis of the Diabetic Wound-Healing Impairment

    PubMed Central

    Xu, Junwang; Wu, Wenjie; Zhang, Liping; Dorset-Martin, Wanda; Morris, Michael W.; Mitchell, Marc E.; Liechty, Kenneth W.

    2012-01-01

    The impairment in diabetic wound healing represents a significant clinical problem. Chronic inflammation is thought to play a central role in the pathogenesis of this impairment. We have previously shown that treatment of diabetic murine wounds with mesenchymal stem cells (MSCs) can improve healing, but the mechanisms are not completely defined. MicroRNA-146a (miR-146a) has been implicated in regulation of the immune and inflammatory responses. We hypothesized that abnormal miRNA-146a expression may contribute to the chronic inflammation. To test this hypothesis, we examined the expression of miRNA-146a and its target genes in diabetic and nondiabetic mice at baseline and after injury. MiR-146a expression was significantly downregulated in diabetic mouse wounds. Decreased miR-146a levels also closely correlated with increased gene expression of its proinflammatory target genes. Furthermore, the correction of the diabetic wound-healing impairment with MSC treatment was associated with a significant increase in the miR-146a expression level and decreased gene expression of its proinflammatory target genes. These results provide the first evidence that decreased expression of miR-146a in diabetic wounds in response to injury may, in part, be responsible for the abnormal inflammatory response seen in diabetic wounds and may contribute to wound-healing impairment. PMID:22851573

  16. 9 CFR 146.22 - Participation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Commercial Table-Egg Layer Flocks § 146.22 Participation. (a) Participating commercial table-egg layer flocks... of subpart B of this part. (b) Commercial table-egg laying premises with fewer than 75,000 birds...

  17. 9 CFR 146.22 - Participation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Commercial Table-Egg Layer Flocks § 146.22 Participation. (a) Participating commercial table-egg layer flocks... of subpart B of this part. (b) Commercial table-egg laying premises with fewer than 75,000 birds...

  18. 9 CFR 146.22 - Participation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Commercial Table-Egg Layer Flocks § 146.22 Participation. (a) Participating commercial table-egg layer flocks... of subpart B of this part. (b) Commercial table-egg laying premises with fewer than 75,000 birds...

  19. 9 CFR 146.22 - Participation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Commercial Table-Egg Layer Flocks § 146.22 Participation. (a) Participating commercial table-egg layer flocks... of subpart B of this part. (b) Commercial table-egg laying premises with fewer than 75,000 birds...

  20. 9 CFR 146.22 - Participation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Commercial Table-Egg Layer Flocks § 146.22 Participation. (a) Participating commercial table-egg layer flocks... of subpart B of this part. (b) Commercial table-egg laying premises with fewer than 75,000 birds...

  1. 7 CFR 1260.146 - Vacancies.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE BEEF PROMOTION AND RESEARCH Beef Promotion and Research Order Cattlemen's Beef Promotion and Research Board § 1260.146 Vacancies. To fill...

  2. 7 CFR 1260.146 - Vacancies.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE BEEF PROMOTION AND RESEARCH Beef Promotion and Research Order Cattlemen's Beef Promotion and Research Board § 1260.146 Vacancies. To fill...

  3. 9 CFR 146.21 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Commercial Table-Egg Layer Flocks § 146.21 Definitions. Table-egg layer. A domesticated chicken grown for the... domesticated chicken grown for the primary purpose of producing eggs for human consumption....

  4. 9 CFR 146.31 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...-Type Chicken Slaughter Plants § 146.31 Definitions. Meat-type chicken. A domesticated chicken grown for... cornish. Meat-type chicken slaughter plant. A meat-type chicken slaughter plant that is...

  5. 9 CFR 146.21 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Commercial Table-Egg Layer Flocks § 146.21 Definitions. Table-egg layer. A domesticated chicken grown for the... domesticated chicken grown for the primary purpose of producing eggs for human consumption....

  6. 9 CFR 146.21 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Commercial Table-Egg Layer Flocks § 146.21 Definitions. Table-egg layer. A domesticated chicken grown for the... domesticated chicken grown for the primary purpose of producing eggs for human consumption....

  7. 9 CFR 146.31 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...-Type Chicken Slaughter Plants § 146.31 Definitions. Meat-type chicken. A domesticated chicken grown for... cornish. Meat-type chicken slaughter plant. A meat-type chicken slaughter plant that is...

  8. 9 CFR 146.31 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-Type Chicken Slaughter Plants § 146.31 Definitions. Meat-type chicken. A domesticated chicken grown for... cornish. Meat-type chicken slaughter plant. A meat-type chicken slaughter plant that is...

  9. 9 CFR 146.31 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...-Type Chicken Slaughter Plants § 146.31 Definitions. Meat-type chicken. A domesticated chicken grown for... cornish. Meat-type chicken slaughter plant. A meat-type chicken slaughter plant that is...

  10. 9 CFR 146.31 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-Type Chicken Slaughter Plants § 146.31 Definitions. Meat-type chicken. A domesticated chicken grown for... cornish. Meat-type chicken slaughter plant. A meat-type chicken slaughter plant that is...

  11. 19 CFR 146.91 - Applicability.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.91 Applicability. This... foreign-trade zone or subzone. Further, the provisions relating to zones generally, which are set...

  12. 9 CFR 146.1 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ..., commercial upland game birds, or commercial waterfowl on one farm. However, at the discretion of the Official... illustrative design, as described in § 146.9 of this part. Service. The Animal and Plant Health...

  13. 9 CFR 146.41 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-Type Turkey Slaughter Plants § 146.41 Definitions. Meat-type turkey. A domesticated turkey grown for the primary purpose of producing meat. Meat-type turkey slaughter plant. A meat-type turkey...

  14. 9 CFR 146.41 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...-Type Turkey Slaughter Plants § 146.41 Definitions. Meat-type turkey. A domesticated turkey grown for the primary purpose of producing meat. Meat-type turkey slaughter plant. A meat-type turkey...

  15. 9 CFR 146.41 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-Type Turkey Slaughter Plants § 146.41 Definitions. Meat-type turkey. A domesticated turkey grown for the primary purpose of producing meat. Meat-type turkey slaughter plant. A meat-type turkey...

  16. 9 CFR 146.41 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...-Type Turkey Slaughter Plants § 146.41 Definitions. Meat-type turkey. A domesticated turkey grown for the primary purpose of producing meat. Meat-type turkey slaughter plant. A meat-type turkey...

  17. 9 CFR 146.41 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...-Type Turkey Slaughter Plants § 146.41 Definitions. Meat-type turkey. A domesticated turkey grown for the primary purpose of producing meat. Meat-type turkey slaughter plant. A meat-type turkey...

  18. 7 CFR 1955.146 - Advertising.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... REGULATIONS (CONTINUED) PROPERTY MANAGEMENT Disposal of Inventory Property General § 1955.146 Advertising. (a... parties. Other innovative means are encouraged, such as the use of a bulletin board to display...

  19. Silencing miR-146a influences B cells and ameliorates experimental autoimmune myasthenia gravis

    PubMed Central

    Zhang, JunMei; Jia, Ge; Liu, Qun; Hu, Jue; Yan, Mei; Yang, BaiFeng; Yang, Huan; Zhou, WenBin; Li, Jing

    2015-01-01

    MicroRNAs have been shown to be important regulators of immune homeostasis as patients with aberrant microRNA expression appeared to be more susceptible to autoimmune diseases. We recently found that miR-146a was up-regulated in activated B cells in response to rat acetylcholine receptor (AChR) α-subunit 97-116 peptide, and this up-regulation was significantly attenuated by AntagomiR-146a. Our data also demonstrated that silencing miR-146a with its inhibitor AntagomiR-146a effectively ameliorated clinical myasthenic symptoms in mice with ongoing experimental autoimmune myasthenia gravis. Furthermore, multiple defects were observed after miR-146a was knocked down in B cells, including decreased anti-R97-116 antibody production and class switching, reduced numbers of plasma cells, memory B cells and B-1 cells, and weakened activation of B cells. Previously, miR-146a has been identified as a nuclear factor-κB-dependent gene and predicted to base pair with the tumour necrosis factor receptor-associated factor 6 (TRAF6) and interleukin-1 receptor-associated kinase 1 (IRAK1) genes to regulate the immune response. However, our study proved that miR-146a inhibition had no effect on the expression of TRAF6 and IRAK1 in B cells. This result suggests that the function of miR-146a in B cells does not involve these two target molecules. We conclude that silencing miR-146a exerts its therapeutic effects by influencing the B-cell functions that contribute to the autoimmune pathogenesis of myasthenia gravis. PMID:24962817

  20. The Mechanism by which 146-N-Glycan Affects the Active Site of Neuraminidase.

    PubMed

    Liu, Pi; Wang, Zhonghua; Zhang, Lijie; Li, Dongmei; Lin, Jianping

    2015-01-01

    One of the most conserved glycosylation sites of neuraminidase (NA) is 146-N-glycan. This site is adjacent to the 150-cavity of NA, which is found within the active site and thought to be a target for rational drug development against the antiviral resistance of influenza. Here, through a total of 2.4 μs molecular dynamics (MD) simulations, we demonstrated that 146-N-glycan can stabilize the conformation of the 150-loop that controls the volume of the 150-cavity. Moreover, with 146-N-glycan, our simulation result was more consistent with crystal structures of NAs than simulations conducted without glycans. Cluster analysis of the MD trajectories showed that 146-N-glycan adopted three distinct conformations: monomer-bridged, dimer-bridged and standing. Of these conformations, the dimer-bridged 146-N-glycan was the most stable one and contributed to stabilization of the 150-loop conformation. Furthermore, our simulation revealed that various standing conformations of 146-N-glycan could block the entrance of the binding pocket. This result was consistent with experimental data and explained the relatively low activity of inhibitors with flexible substituents toward the 150-cavity. Together, our results lead us to hypothesize that rigid and hydrophobic substituents could serve as better inhibitors targeting the 150-cavity. PMID:26267136

  1. Do foraminifera accurately record seawater neodymium isotope composition?

    NASA Astrophysics Data System (ADS)

    Scrivner, Adam; Skinner, Luke; Vance, Derek

    2010-05-01

    Palaeoclimate studies involving the reconstruction of past Atlantic meridional overturning circulation increasingly employ isotopes of neodymium (Nd), measured on a variety of sample media (Frank, 2002). In the open ocean, Nd isotopes are a conservative tracer of water mass mixing and are unaffected by biological and low-temperature fractionation processes (Piepgras and Wasserburg, 1987; Lacan and Jeandel, 2005). For decades, benthic foraminifera have been widely utilised in stable isotope and geochemical studies, but have only recently begun to be exploited as a widely distributed, high-resolution Nd isotope archive (Klevenz et al., 2008), potentially circumventing the difficulties associated with other methods used to recover past deep-water Nd isotopes (Klevenz et al., 2008; Rutberg et al., 2000; Tachikawa et al., 2004). Thus far, a single pilot study (Klevenz et al., 2008) has indicated that core-top sedimentary benthic foraminifera record a Nd isotope composition in agreement with the nearest available bottom seawater data, and has suggested that this archive is potentially useful on both millennial and million-year timescales. Here we present seawater and proximal core-top foraminifer Nd isotope data for samples recovered during the 2008 "RETRO" cruise of the Marion Dufresne. The foraminifer samples comprise a depth-transect spanning 3000m of the water column in the Angola Basin and permit a direct comparison between high-resolution water column and core-top foraminiferal Nd isotope data. We use these data to assess the reliability of both planktonic and benthic foraminifera as recorders of water column neodymium isotope composition. Frank, M., 2002. Radiogenic isotopes: Tracers of past ocean circulation and erosional input, Rev. Geophys., 40 (1), 1001, doi:10.1029/2000RG000094. Klevenz, V., Vance, D., Schmidt, D.N., and Mezger, K., 2008. Neodymium isotopes in benthic foraminifera: Core-top systematics and a down-core record from the Neogene south Atlantic

  2. Effect of transscleral neodymium: YAG cyclophotocoagulation on intraocular lenses

    SciTech Connect

    Blomquist, P.H.; Gross, R.L.; Koch, D.D. )

    1990-03-01

    A neodymium: YAG laser operating in the thermal mode was used to irradiate isolated intraocular lenses (IOLs) and to perform transscleral cyclophotocoagulation on pseudophakic autopsy eyes to investigate the potential damage to IOL haptics such irradiation may cause. In the isolated IOLs, 70 mJ of energy deformed and partially melted both polymethylmethacrylate (PMMA) and polypropylene haptics. One of the capsular-fixated PC-IOL haptics in an autopsy eye partially melted when irradiated with the maximum energy level (8.8 J), with the aiming beam focused 1 mm posterior to the limbus and maximal posterior focus offset.

  3. Local immunity in treating skin melanoma by neodymium pulsed laser

    NASA Astrophysics Data System (ADS)

    Moskalik, Konstantin G.

    1997-06-01

    The number and correlation of skin stroma cells was studied on mice C57B1 with the subcutaneously transplanted melanoma B16 which was exposed to neodymium pulsed laser radiation. Within 1-5 days after the exposure the total number of the free skin stroma cells was found to increase in the periphery from the radiation epicenter and the number of lymphocytes, macrophages and leucocytes tended to grow. Lymphoid infiltration was also revealed in the preparations of the epithelized wound and cicatrix on the skin melanoma sites in the patients who had undergone pulsed laser radiation therapy.

  4. Urethral strictures treatment with neodymium:YAG laser.

    PubMed

    Silber, N

    1992-04-01

    A total of 14 patients with stricture of the urethra underwent treatment with Nd:YAG (neodymium:YAG) laser irradiation. A new 800-micron hemispherical optical quartz fiber was used in contact technique to produce linear incisions in the scarred tissue. Within 11.2 months, median of follow up, there was improvement in the obstructive voiding symptoms in all the patients. One patient who still had mild stricture in the first follow-up cystoscopy was managed successfully with second treatment. Endoscopic application of laser energy in the contact mode facilitates the immediate vaporization and disintegration of the fibrous area and secondary reepithelization of the urethra without scarring. PMID:10171965

  5. 21 CFR 146.140 - Pasteurized orange juice.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Pasteurized orange juice. 146.140 Section 146.140... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared from unfermented juice obtained from mature oranges as specified in § 146.135, to which may be...

  6. 21 CFR 146.150 - Canned concentrated orange juice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Canned concentrated orange juice. 146.150 Section... Fruit Juices and Beverages § 146.150 Canned concentrated orange juice. (a) Canned concentrated orange... labeling of ingredients prescribed for frozen concentrated orange juice by § 146.146, except that it is...

  7. 21 CFR 146.150 - Canned concentrated orange juice.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Canned concentrated orange juice. 146.150 Section... Fruit Juices and Beverages § 146.150 Canned concentrated orange juice. (a) Canned concentrated orange... labeling of ingredients prescribed for frozen concentrated orange juice by § 146.146, except that it is...

  8. 21 CFR 146.150 - Canned concentrated orange juice.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Canned concentrated orange juice. 146.150 Section... Fruit Juices and Beverages § 146.150 Canned concentrated orange juice. (a) Canned concentrated orange... labeling of ingredients prescribed for frozen concentrated orange juice by § 146.146, except that it is...

  9. 21 CFR 146.140 - Pasteurized orange juice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Pasteurized orange juice. 146.140 Section 146.140... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared from unfermented juice obtained from mature oranges as specified in § 146.135, to which may be...

  10. 21 CFR 146.150 - Canned concentrated orange juice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned concentrated orange juice. 146.150 Section... Fruit Juices and Beverages § 146.150 Canned concentrated orange juice. (a) Canned concentrated orange... labeling of ingredients prescribed for frozen concentrated orange juice by § 146.146, except that it is...

  11. 21 CFR 146.150 - Canned concentrated orange juice.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Canned concentrated orange juice. 146.150 Section... Fruit Juices and Beverages § 146.150 Canned concentrated orange juice. (a) Canned concentrated orange... labeling of ingredients prescribed for frozen concentrated orange juice by § 146.146, except that it is...

  12. 21 CFR 146.140 - Pasteurized orange juice.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Pasteurized orange juice. 146.140 Section 146.140... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared from unfermented juice obtained from mature oranges as specified in § 146.135, to which may be...

  13. 21 CFR 146.140 - Pasteurized orange juice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Pasteurized orange juice. 146.140 Section 146.140... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared from unfermented juice obtained from mature oranges as specified in § 146.135, to which may be...

  14. 21 CFR 146.140 - Pasteurized orange juice.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Pasteurized orange juice. 146.140 Section 146.140... and Beverages § 146.140 Pasteurized orange juice. (a) Pasteurized orange juice is the food prepared from unfermented juice obtained from mature oranges as specified in § 146.135, to which may be...

  15. miR-146a inhibits cell growth, cell migration and induces apoptosis in non-small cell lung cancer cells.

    PubMed

    Chen, Gang; Umelo, Ijeoma Adaku; Lv, Shasha; Teugels, Erik; Fostier, Karel; Kronenberger, Peter; Dewaele, Alex; Sadones, Jan; Geers, Caroline; De Grève, Jacques

    2013-01-01

    Aberrant expression of microRNA-146a (miR-146a) has been reported to be involved in the development and progression of various types of cancers. However, its role in non-small cell lung cancer (NSCLC) has not been elucidated. The aim of this study was to investigate the contribution of miR-146a to various aspects of the malignant phenotype of human NSCLCs. In functional experiments, miR-146a suppressed cell growth, induced cellular apoptosis and inhibited EGFR downstream signaling in five NSCLC cell lines (H358, H1650, H1975, HCC827 and H292). miR-146a also inhibited the migratory capacity of these NSCLC cells. On the other hand, miR-146a enhanced the inhibition of cell proliferation by drugs targeting EGFR, including both TKIs (gefitinib, erlotinib, and afatinib) and a monoclonal antibody (cetuximab). These effects were independent of the EGFR mutation status (wild type, sensitizing mutation or resistance mutation), but were less potent compared to the effects of siRNA targeting of EGFR. Our results suggest that these effects of miR-146a are due to its targeting of EGFR and NF-κB signaling. We also found, in clinical formalin fixed paraffin embedded (FFPE) lung cancer samples, that low expression of miR-146a was correlated with advanced clinical TNM stages and distant metastasis in NSCLC (P<0.05). The patients with high miR-146a expression in their tumors showed longer progression-free survival (25.6 weeks in miR-146a high patients vs. 4.8 weeks in miR-146a low patients, P<0.05). miR-146a is therefore a strong candidate prognostic biomarker in NSCLC. Thus inducing miR-146a might be a therapeutic strategy for NSCLC. PMID:23555954

  16. 21 CFR 146.141 - Canned orange juice.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Canned orange juice. 146.141 Section 146.141 Food... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as specified in § 146.135 or frozen orange juice as specified in § 146.137, or a combination of both, to...

  17. 21 CFR 146.141 - Canned orange juice.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Canned orange juice. 146.141 Section 146.141 Food... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as specified in § 146.135 or frozen orange juice as specified in § 146.137, or a combination of both, to...

  18. 21 CFR 146.141 - Canned orange juice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Canned orange juice. 146.141 Section 146.141 Food... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as specified in § 146.135 or frozen orange juice as specified in § 146.137, or a combination of both, to...

  19. 21 CFR 146.141 - Canned orange juice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Canned orange juice. 146.141 Section 146.141 Food... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as specified in § 146.135 or frozen orange juice as specified in § 146.137, or a combination of both, to...

  20. 21 CFR 146.141 - Canned orange juice.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Canned orange juice. 146.141 Section 146.141 Food... Beverages § 146.141 Canned orange juice. (a) Canned orange juice is the food prepared from orange juice as specified in § 146.135 or frozen orange juice as specified in § 146.137, or a combination of both, to...

  1. Incorporation of Cerium and Neodymium in Uranyl Phases

    SciTech Connect

    Kim, C W.; Wronkiewicz, David J.; Finch, R J.; Buck, Edgar C.

    2006-07-15

    The potential for incorporating rare earth elements (REE) into/onto crystalline compounds has been evaluated by precipitating uranyl phases from aqueous solutions containing either cerium or neodymium. These REEs serve both as monitors for evaluating the potential repository behavior of REE radionuclides, and as surrogate elements for actinides (e.g., Ce4 and Nd3 for Pu4 and Am3, respectively). The present experiments examined the behavior of REE in the presence of ianthinite Formula Not Shown, becquerelite (Ca(UO2)6O4(OH)6(H2O)8), and other uranyl hydroxide compounds commonly noted as alteration products during the corrosion of UO2, spent nuclear fuel, and naturally occurring uraninite. The results of these experiments demonstrate that significant quantities of both cerium (Kd=1020) and neodymium (Kd=840) are incorporated within the uranium alteration phases and suggest that ionic substitution and/or adsorption to the uranyl phases can play a key role in the limiting the mobility of REE (and by analogy, actinide elements) in a nuclear waste repository.

  2. Recycling potential of neodymium: the case of computer hard disk drives.

    PubMed

    Sprecher, Benjamin; Kleijn, Rene; Kramer, Gert Jan

    2014-08-19

    Neodymium, one of the more critically scarce rare earth metals, is often used in sustainable technologies. In this study, we investigate the potential contribution of neodymium recycling to reducing scarcity in supply, with a case study on computer hard disk drives (HDDs). We first review the literature on neodymium production and recycling potential. From this review, we find that recycling of computer HDDs is currently the most feasible pathway toward large-scale recycling of neodymium, even though HDDs do not represent the largest application of neodymium. We then use a combination of dynamic modeling and empirical experiments to conclude that within the application of NdFeB magnets for HDDs, the potential for loop-closing is significant: up to 57% in 2017. However, compared to the total NdFeB production capacity, the recovery potential from HDDs is relatively small (in the 1-3% range). The distributed nature of neodymium poses a significant challenge for recycling of neodymium. PMID:25029356

  3. Differential expression of miRNA-146a-regulated inflammatory genes in human primary neural, astroglial and microglial cells

    PubMed Central

    Li, Yuan Yuan; Cui, Jian Guo; Dua, Prerna; Pogue, Aileen I.; Bhattacharjee, Surjyadipta; Lukiw, Walter J.

    2013-01-01

    Micro RNA-146a (miRNA-146a) is an inducible, 22 nucleotide, small RNA over-expressed in Alzheimer’s disease (AD) brain. Up-regulated miRNA-146a targets several inflammation-related and membrane-associated messenger RNAs (mRNAs), including those encoding complement factor-H (CFH) and the interleukin-1 receptor associated kinase-1 (IRAK-1), resulting in significant decreases in their expression (p < 0.05, ANOVA). In this study we assayed miRNA-146a, CFH, IRAK-1 and tetraspanin-12 (TSPAN12), abundances in primary human neuronal-glial (HNG) co-cultures, in human astroglial (HAG) and microglial (HMG) cells stressed with Aβ42 peptide and tumor necrosis factor alpha (TNFα). The results indicate a consistent inverse relationship between miRNA-146a and CFH, IRAK-1 and TSPAN12 expression levels, and indicate that HNG, HAG and HMG cell types each respond differently to Aβ42-peptide + TNFα-triggered stress. While the strongest miRNA-146a-IRAK-1 response was found in HAG cells, the largest miRNA-146a-TSPAN12 response was found in HNG cells, and the most significant miRNA-146a-CFH changes were found in HMG cells, the ‘resident scavenging macrophages’ of the brain. PMID:21640790

  4. Electrolytic production of neodymium without perfluorinated carbon compounds on the offgases

    DOEpatents

    Keller, Rudolf; Larimer, Kirk T.

    1998-01-01

    A method of producing neodymium in an electrolytic cell without formation of perfluorinated carbon gases (PFCs), the method comprising the steps of providing an electrolyte in the electrolytic cell and providing an anode in an anode region of the electrolyte and providing a cathode in a cathode region of the electrolytic cell. Dissolving an oxygen-containing neodymium compound in the electrolyte in the anode region and maintaining a more intense electrolyte circulation in the anode region than in the cathode region. Passing an electrolytic current between said anode and said cathode and depositing neodymium metal at the cathode, preventing the formation of perfluorinated carbon gases by limiting anode over voltage.

  5. Origin of the Sudbury Complex by meteoritic impact: Neodymium isotopic evidence

    USGS Publications Warehouse

    Faggart, B.E., Jr.; Basu, A.R.; Tatsumoto, M.

    1985-01-01

    Samarium-neodymium isotopic data on whole rocks and minerals of the Sudbury Complex in Canada gave an igneous crystallization age of 1840 ?? 21 ?? 106 years. The initial epsilon neodymium values for 15 whole rocks are similar to those for average upper continental crust, falling on the crustal trend of neodymium isotopic evolution as defined by shales. The rare earth element concentration patterns of Sudbury rocks are also similar to upper crustal averages. These data suggest that the Sudbury Complex formed from melts generated in the upper crust and are consistent with a meteoritic impact.

  6. 19 CFR 146.43 - Domestic status.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.43 Domestic status. (a) General... and repair material, to a zone, except upon order of the Commissioner of Customs. No application or... mixed or combined with merchandise in another zone status, or (2) upon order of the Commissioner...

  7. 19 CFR 146.43 - Domestic status.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.43 Domestic status. (a) General... and repair material, to a zone, except upon order of the Commissioner of Customs. No application or... mixed or combined with merchandise in another zone status, or (2) upon order of the Commissioner...

  8. 19 CFR 146.43 - Domestic status.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.43 Domestic status. (a) General... and repair material, to a zone, except upon order of the Commissioner of Customs. No application or... mixed or combined with merchandise in another zone status, or (2) upon order of the Commissioner...

  9. 19 CFR 146.43 - Domestic status.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.43 Domestic status. (a) General... and repair material, to a zone, except upon order of the Commissioner of Customs. No application or... mixed or combined with merchandise in another zone status, or (2) upon order of the Commissioner...

  10. 19 CFR 146.7 - Zone changes.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the risk and expense of the operator. The port director may require an accounting of all merchandise... surety; or (4) for any other reason that substantially affects the liability of the operator under the... (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.7 Zone changes. (a) Alteration of an activated...

  11. 9 CFR 146.32 - Participation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...-Type Chicken Slaughter Plants § 146.32 Participation. (a) Participating meat-type chicken slaughter... provisions of this subpart C. (b) Meat-type chicken slaughter plants that slaughter fewer than 200,000 meat-type chickens in an operating week are exempt from the special provisions of this subpart C....

  12. 9 CFR 146.32 - Participation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...-Type Chicken Slaughter Plants § 146.32 Participation. (a) Participating meat-type chicken slaughter... provisions of this subpart C. (b) Meat-type chicken slaughter plants that slaughter fewer than 200,000 meat-type chickens in an operating week are exempt from the special provisions of this subpart C....

  13. 9 CFR 146.32 - Participation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-Type Chicken Slaughter Plants § 146.32 Participation. (a) Participating meat-type chicken slaughter... provisions of this subpart C. (b) Meat-type chicken slaughter plants that slaughter fewer than 200,000 meat-type chickens in an operating week are exempt from the special provisions of this subpart C....

  14. 9 CFR 146.32 - Participation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-Type Chicken Slaughter Plants § 146.32 Participation. (a) Participating meat-type chicken slaughter... provisions of this subpart C. (b) Meat-type chicken slaughter plants that slaughter fewer than 200,000 meat-type chickens in an operating week are exempt from the special provisions of this subpart C....

  15. 9 CFR 146.32 - Participation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...-Type Chicken Slaughter Plants § 146.32 Participation. (a) Participating meat-type chicken slaughter... provisions of this subpart C. (b) Meat-type chicken slaughter plants that slaughter fewer than 200,000 meat-type chickens in an operating week are exempt from the special provisions of this subpart C....

  16. 19 CFR 146.92 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... products to feedstocks for petroleum manufacturing in accordance with the Industry Standards of Potential... (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.92 Definitions. (a.... “Feedstocks” means crude petroleum or intermediate product that is used in a petroleum refinery to make...

  17. 19 CFR 146.92 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... products to feedstocks for petroleum manufacturing in accordance with the Industry Standards of Potential... (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.92 Definitions. (a.... “Feedstocks” means crude petroleum or intermediate product that is used in a petroleum refinery to make...

  18. 19 CFR 146.92 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... products to feedstocks for petroleum manufacturing in accordance with the Industry Standards of Potential... (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.92 Definitions. (a.... “Feedstocks” means crude petroleum or intermediate product that is used in a petroleum refinery to make...

  19. 19 CFR 146.92 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... products to feedstocks for petroleum manufacturing in accordance with the Industry Standards of Potential... (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.92 Definitions. (a.... “Feedstocks” means crude petroleum or intermediate product that is used in a petroleum refinery to make...

  20. 40 CFR 146.71 - Closure.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... operator shall observe and record the pressure decay for a time specified by the Director. The Director shall analyze the pressure decay and the transient pressure observations conducted pursuant to § 146.68.... Testing methods may include: (i) Pressure tests with liquid or gas; (ii) Radioactive tracer surveys;...

  1. 40 CFR 146.71 - Closure.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... operator shall observe and record the pressure decay for a time specified by the Director. The Director shall analyze the pressure decay and the transient pressure observations conducted pursuant to § 146.68.... Testing methods may include: (i) Pressure tests with liquid or gas; (ii) Radioactive tracer surveys;...

  2. 40 CFR 146.71 - Closure.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... operator shall observe and record the pressure decay for a time specified by the Director. The Director shall analyze the pressure decay and the transient pressure observations conducted pursuant to § 146.68.... Testing methods may include: (i) Pressure tests with liquid or gas; (ii) Radioactive tracer surveys;...

  3. 40 CFR 146.51 - Applicability.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... in 10 CFR part 20, appendix B, table II, column 2. ... Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND... sources of drinking water. It includes all wells listed in § 146.5(e) but is not limited to those types...

  4. 22 CFR 146.310 - Recruitment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Admission and....300 through 146.310 apply shall not discriminate on the basis of sex in the recruitment and...

  5. 22 CFR 146.300 - Admission.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Discrimination on the Basis of Sex in Admission and Recruitment Prohibited § 146.300 Admission. (a) General. No person shall, on the basis of sex, be...

  6. 27 CFR 44.146 - Closing.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2010-04-01 2010-04-01 false Closing. 44.146 Section 44... Closing. A closing inventory shall be made by the export warehouse proprietor when he transfers ownership or concludes business. Where the proprietor transfers ownership the closing inventory shall be...

  7. 27 CFR 44.146 - Closing.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2014-04-01 2014-04-01 false Closing. 44.146 Section 44... Closing. A closing inventory shall be made by the export warehouse proprietor when he transfers ownership or concludes business. Where the proprietor transfers ownership the closing inventory shall be...

  8. 27 CFR 44.146 - Closing.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2011-04-01 2011-04-01 false Closing. 44.146 Section 44... Closing. A closing inventory shall be made by the export warehouse proprietor when he transfers ownership or concludes business. Where the proprietor transfers ownership the closing inventory shall be...

  9. 27 CFR 44.146 - Closing.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2012-04-01 2011-04-01 true Closing. 44.146 Section 44... Closing. A closing inventory shall be made by the export warehouse proprietor when he transfers ownership or concludes business. Where the proprietor transfers ownership the closing inventory shall be...

  10. 27 CFR 44.146 - Closing.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 2 2013-04-01 2013-04-01 false Closing. 44.146 Section 44... Closing. A closing inventory shall be made by the export warehouse proprietor when he transfers ownership or concludes business. Where the proprietor transfers ownership the closing inventory shall be...

  11. 9 CFR 146.42 - Participation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...-Type Turkey Slaughter Plants § 146.42 Participation. (a) Participating meat-type turkey slaughter... provisions of this subpart D. (b) Meat-type turkey slaughter plants that slaughter fewer than 2 million meat-type turkeys in a 12-month period are exempt from the special provisions of this subpart D....

  12. 9 CFR 146.42 - Participation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...-Type Turkey Slaughter Plants § 146.42 Participation. (a) Participating meat-type turkey slaughter... provisions of this subpart D. (b) Meat-type turkey slaughter plants that slaughter fewer than 2 million meat-type turkeys in a 12-month period are exempt from the special provisions of this subpart D....

  13. 9 CFR 146.42 - Participation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...-Type Turkey Slaughter Plants § 146.42 Participation. (a) Participating meat-type turkey slaughter... provisions of this subpart D. (b) Meat-type turkey slaughter plants that slaughter fewer than 2 million meat-type turkeys in a 12-month period are exempt from the special provisions of this subpart D....

  14. 9 CFR 146.42 - Participation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...-Type Turkey Slaughter Plants § 146.42 Participation. (a) Participating meat-type turkey slaughter... provisions of this subpart D. (b) Meat-type turkey slaughter plants that slaughter fewer than 2 million meat-type turkeys in a 12-month period are exempt from the special provisions of this subpart D....

  15. 9 CFR 146.42 - Participation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ...-Type Turkey Slaughter Plants § 146.42 Participation. (a) Participating meat-type turkey slaughter... provisions of this subpart D. (b) Meat-type turkey slaughter plants that slaughter fewer than 2 million meat-type turkeys in a 12-month period are exempt from the special provisions of this subpart D....

  16. 40 CFR 146.89 - Mechanical integrity.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... tubing and long-string casing; and annulus fluid volume as specified in § 146.88 (e); (c) At least once... inspection log to determine the presence or absence of corrosion in the long-string casing. (e) The Director... test data submitted since the previous evaluation. (g) The Director may require additional...

  17. 40 CFR 146.89 - Mechanical integrity.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... tubing and long-string casing; and annulus fluid volume as specified in § 146.88 (e); (c) At least once... inspection log to determine the presence or absence of corrosion in the long-string casing. (e) The Director... test data submitted since the previous evaluation. (g) The Director may require additional...

  18. 40 CFR 146.89 - Mechanical integrity.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... tubing and long-string casing; and annulus fluid volume as specified in § 146.88 (e); (c) At least once... inspection log to determine the presence or absence of corrosion in the long-string casing. (e) The Director... test data submitted since the previous evaluation. (g) The Director may require additional...

  19. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of Investigation, the Director of the Bureau of Prisons, the Commissioner of Federal Prison Industries, the... Drug Enforcement Administration, the Director of the Bureau of Alcohol, Tobacco, Firearms,...

  20. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of Investigation, the Director of the Bureau of Prisons, the Commissioner of Federal Prison Industries, the... Drug Enforcement Administration, the Director of the Bureau of Alcohol, Tobacco, Firearms,...

  1. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of Investigation, the Director of the Bureau of Prisons, the Commissioner of Federal Prison Industries, the... Drug Enforcement Administration, the Director of the Bureau of Alcohol, Tobacco, Firearms,...

  2. 28 CFR 0.146 - Seals.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Personnel and Certain Administrative Matters § 0.146 Seals. The Director of the Federal Bureau of Investigation, the Director of the Bureau of Prisons, the Commissioner of Federal Prison Industries, the... Drug Enforcement Administration, the Director of the Bureau of Alcohol, Tobacco, Firearms,...

  3. 9 CFR 146.51 - Definitions.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.51 Definitions. Commercial upland game bird slaughter plant. A commercial upland game bird slaughter plant that is federally inspected or under State inspection that the...

  4. 9 CFR 146.52 - Participation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.52 Participation. (a) Participating commercial upland game bird slaughter plants, commercial waterfowl slaughter plants, raised-for-release upland game bird premises, and...

  5. 9 CFR 146.51 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.51 Definitions. Commercial upland game bird slaughter plant. A commercial upland game bird slaughter plant that is federally inspected or under State inspection that the...

  6. 9 CFR 146.51 - Definitions.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.51 Definitions. Commercial upland game bird slaughter plant. A commercial upland game bird slaughter plant that is federally inspected or under State inspection that the...

  7. 9 CFR 146.52 - Participation.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.52 Participation. (a) Participating commercial upland game bird slaughter plants, commercial waterfowl slaughter plants, raised-for-release upland game bird premises, and...

  8. 9 CFR 146.52 - Participation.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.52 Participation. (a) Participating commercial upland game bird slaughter plants, commercial waterfowl slaughter plants, raised-for-release upland game bird premises, and...

  9. 9 CFR 146.51 - Definitions.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.51 Definitions. Commercial upland game bird slaughter plant. A commercial upland game bird slaughter plant that is federally inspected or under State inspection that the...

  10. 9 CFR 146.52 - Participation.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.52 Participation. (a) Participating commercial upland game bird slaughter plants, commercial waterfowl slaughter plants, raised-for-release upland game bird premises, and...

  11. 9 CFR 146.51 - Definitions.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.51 Definitions. Commercial upland game bird slaughter plant. A commercial upland game bird slaughter plant that is federally inspected or under State inspection that the...

  12. 9 CFR 146.52 - Participation.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... Commercial Upland Game Birds, Commercial Waterfowl, Raised-for-Release Upland Game Birds, and Raised-for-Release Waterfowl § 146.52 Participation. (a) Participating commercial upland game bird slaughter plants, commercial waterfowl slaughter plants, raised-for-release upland game bird premises, and...

  13. 40 CFR 146.32 - Construction requirements.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Section 146.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS... cemented to prevent the migration of fluids into or between underground sources of drinking water. The... projects where he has substantial evidence that no contamination of underground sources of drinking...

  14. 40 CFR 146.32 - Construction requirements.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Section 146.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS... cemented to prevent the migration of fluids into or between underground sources of drinking water. The... projects where he has substantial evidence that no contamination of underground sources of drinking...

  15. 40 CFR 146.32 - Construction requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 146.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS... cemented to prevent the migration of fluids into or between underground sources of drinking water. The... projects where he has substantial evidence that no contamination of underground sources of drinking...

  16. 40 CFR 146.22 - Construction requirements.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Section 146.22 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS... drinking water. The casing and cement used in the construction of each newly drilled well shall be designed... fluids into an underground source of drinking water so as to create a significant risk to the health...

  17. 40 CFR 146.32 - Construction requirements.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Section 146.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS... cemented to prevent the migration of fluids into or between underground sources of drinking water. The... projects where he has substantial evidence that no contamination of underground sources of drinking...

  18. 40 CFR 146.32 - Construction requirements.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Section 146.32 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS... cemented to prevent the migration of fluids into or between underground sources of drinking water. The... projects where he has substantial evidence that no contamination of underground sources of drinking...

  19. 22 CFR 146.300 - Admission.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... discriminate against or exclude any person on the basis of pregnancy, childbirth, termination of pregnancy, or...) Subject to § 146.235(d), shall treat disabilities related to pregnancy, childbirth, termination of pregnancy, or recovery therefrom in the same manner and under the same policies as any other...

  20. A new contact neodymium: YAG laser for cyclophotocoagulation

    SciTech Connect

    Iwach, A.G.; Drake, M.V.; Hoskins, H.D. Jr.; Schuster, B.L.; Vassiliadis, A.; Crawford, J.B.; Hennings, D.R. )

    1991-06-01

    A newly developed compact (40 kg), self-contained contact Neodymium:YAG laser produces high-peak, high-energy (800 mJ/pulse), short (1.0 millisecond) pulses with 1 to 3 pulses/exposure. Energy is delivered via a 320-microns cleaved quartz fiber optic probe. Cyclophotocoagulation was performed in five eyes of three medium-sized Dutch-pigmented rabbits. The eyes received exposures of 1 to 3 pulses/exposure. Energy delivered ranged from 100 to 800 mJ/pulse. Histopathology revealed ciliary body disruption and hemorrhage with no damage to overlying sclera. When used for transscleral cyclodiathermy in the rabbit, the laser created significant ciliary body disruption with minimal scleral injury.

  1. Neodymium isotope evidence for a chondritic composition of the Moon.

    PubMed

    Rankenburg, K; Brandon, A D; Neal, C R

    2006-06-01

    Samarium-neodymium isotope data for six lunar basalts show that the bulk Moon has a 142Nd/144Nd ratio that is indistinguishable from that of chondritic meteorites but is 20 parts per million less than most samples from Earth. The Sm/Nd formation interval of the lunar mantle from these data is 215(-21)(+23) million years after the onset of solar system condensation. Because both Earth and the Moon likely formed in the same region of the solar nebula, Earth should also have a chondritic bulk composition. In order to mass balance the Nd budget, these constraints require that a complementary reservoir with a lower 142Nd/144Nd value resides in Earth's mantle. PMID:16741118

  2. α-uranium phase in compressed neodymium metal

    NASA Astrophysics Data System (ADS)

    Chesnut, Gary N.; Vohra, Yogesh K.

    2000-02-01

    The light rare-earth metal, neodymium, has been studied up to 155 GPa in a diamond-anvil cell using energy dispersive x-ray diffraction with a synchrotron source. The pressures were calibrated using copper as an internal x-ray pressure standard. A phase transformation from a monoclinic phase (C2/m, 4 atoms/cell) to an orthorhombic α-U phase (Cmcm, 4 atoms/cell) was observed at 113+/-6 GPa without any observable volume collapse. The observation of α-U phase in Nd and, previously, in cerium and praseodymium clearly establishes this phase in light rare-earth metals. Our equation of state measurements suggest that delocalization of the f shell in Nd occurs without any volume collapse unlike Ce and Pr.

  3. Isotope enrichment by frequency-tripled temperature tuned neodymium laser photolysis of formaldehyde

    DOEpatents

    Marling, John B.

    1977-01-01

    Enrichment of carbon, hydrogen and/or oxygen isotopes by means of isotopically selective photo-predissociation of formaldehyde is achieved by irradiation provided by a frequency-tripled, temperature tuned neodymium laser.

  4. Microscale Demonstration of the Paramagnetism of Liquid Oxygen with a Neodymium Magnet

    ERIC Educational Resources Information Center

    Mattson, Bruce

    2007-01-01

    A microscale classroom demonstration of the paramagnetic behavior of various samples of liquid oxygen with neodymium magnet is being presented. The experiment should be done with extreme caution, as liquid oxygen reacts violently with organic matters.

  5. Description of the traction characteristics of the neodymium compensators of the automatic vibration isolations

    NASA Astrophysics Data System (ADS)

    Gurova, E. G.; Panchenko, Y. V.; Gurov, M. G.

    2016-04-01

    In this paper the method of calculation of neodymium magnets was presented. The calculation of the neodymium magnets characteristics and stiffness correctors of the vibration isolator according to the requirements for vibration isolation devices with stiffness compensators was performed. This research has been performed with the support of the President scholarship for young scientists, order No. 184 of Ministry of education and science of the Russian Federation of the 10th of March 2015.

  6. 21 CFR 146.137 - Frozen orange juice.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Frozen orange juice. 146.137 Section 146.137 Food... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135, except that it is frozen. (b) The name of the food is “Frozen orange juice”. Such name may be preceded...

  7. 21 CFR 146.137 - Frozen orange juice.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen orange juice. 146.137 Section 146.137 Food... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135, except that it is frozen. (b) The name of the food is “Frozen orange juice”. Such name may be preceded...

  8. 21 CFR 146.137 - Frozen orange juice.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen orange juice. 146.137 Section 146.137 Food... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135, except that it is frozen. (b) The name of the food is “Frozen orange juice”. Such name may be preceded...

  9. 21 CFR 146.137 - Frozen orange juice.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Frozen orange juice. 146.137 Section 146.137 Food... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135, except that it is frozen. (b) The name of the food is “Frozen orange juice”. Such name may be preceded...

  10. 21 CFR 146.137 - Frozen orange juice.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Frozen orange juice. 146.137 Section 146.137 Food... Beverages § 146.137 Frozen orange juice. (a) Frozen orange juice is orange juice as defined in § 146.135, except that it is frozen. (b) The name of the food is “Frozen orange juice”. Such name may be preceded...

  11. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 9 Animals and Animal Products 1 2011-01-01 2011-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR COMMERCIAL POULTRY General Provisions § 146.8...

  12. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 9 Animals and Animal Products 1 2014-01-01 2014-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR COMMERCIAL POULTRY General Provisions § 146.8...

  13. 34 CFR 303.146 - Information about public participation.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 34 Education 2 2011-07-01 2010-07-01 true Information about public participation. 303.146 Section 303.146 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF... Application § 303.146 Information about public participation. Each application must include the information...

  14. 19 CFR 146.13 - Customs forms and procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Customs forms and procedures. 146.13 Section 146.13 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.13 Customs forms and...

  15. 19 CFR 146.51 - Customs control of merchandise.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Customs control of merchandise. 146.51 Section 146.51 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Handling of Merchandise in a Zone § 146.51...

  16. 22 CFR 146.415 - Access to course offerings.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Access to course offerings. 146.415 Section 146.415 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN... in Education Programs or Activities Prohibited § 146.415 Access to course offerings. (a) A...

  17. 14 CFR 21.146 - Responsibility of holder.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 14 Aeronautics and Space 1 2014-01-01 2014-01-01 false Responsibility of holder. 21.146 Section 21.146 Aeronautics and Space FEDERAL AVIATION ADMINISTRATION, DEPARTMENT OF TRANSPORTATION AIRCRAFT CERTIFICATION PROCEDURES FOR PRODUCTS AND PARTS Production Certificates § 21.146 Responsibility of holder. The holder of a production certificate...

  18. 19 CFR 146.13 - Customs forms and procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Customs forms and procedures. 146.13 Section 146.13 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.13 Customs forms and...

  19. 19 CFR 146.13 - Customs forms and procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Customs forms and procedures. 146.13 Section 146.13 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.13 Customs forms and...

  20. 19 CFR 146.51 - Customs control of merchandise.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Customs control of merchandise. 146.51 Section 146.51 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Handling of Merchandise in a Zone § 146.51...

  1. 19 CFR 146.51 - Customs control of merchandise.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Customs control of merchandise. 146.51 Section 146.51 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Handling of Merchandise in a Zone § 146.51...

  2. 22 CFR 146.600 - Notice of covered programs.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Notice of covered programs. 146.600 Section 146.600 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Procedures § 146.600 Notice of covered programs. Within 60 days of September...

  3. 37 CFR 1.146 - Election of species.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2010-07-01 2010-07-01 false Election of species. 1.146 Section 1.146 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF... One Application; Restriction § 1.146 Election of species. In the first action on an...

  4. 37 CFR 1.146 - Election of species.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 37 Patents, Trademarks, and Copyrights 1 2013-07-01 2013-07-01 false Election of species. 1.146 Section 1.146 Patents, Trademarks, and Copyrights UNITED STATES PATENT AND TRADEMARK OFFICE, DEPARTMENT OF... One Application; Restriction § 1.146 Election of species. In the first action on an...

  5. 19 CFR 146.42 - Nonprivileged foreign status.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Nonprivileged foreign status. 146.42 Section 146... THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.42 Nonprivileged foreign status. All of the following will have the status of nonprivileged foreign merchandise:...

  6. 19 CFR 146.41 - Privileged foreign status.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Privileged foreign status. 146.41 Section 146.41... TREASURY (CONTINUED) FOREIGN TRADE ZONES Status of Merchandise in a Zone § 146.41 Privileged foreign status. (a) General. Foreign merchandise which has not been manipulated or manufactured so as to effect...

  7. 7 CFR 1703.146 - Submission of applications.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 11 2014-01-01 2014-01-01 false Submission of applications. 1703.146 Section 1703.146 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan Program § 1703.146 Submission...

  8. 7 CFR 1703.146 - Submission of applications.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 11 2012-01-01 2012-01-01 false Submission of applications. 1703.146 Section 1703.146 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan Program § 1703.146 Submission...

  9. 7 CFR 1703.146 - Submission of applications.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 11 2010-01-01 2010-01-01 false Submission of applications. 1703.146 Section 1703.146 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan Program § 1703.146 Submission...

  10. 19 CFR 146.13 - Customs forms and procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Customs forms and procedures. 146.13 Section 146.13 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.13 Customs forms and...

  11. 19 CFR 146.13 - Customs forms and procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Customs forms and procedures. 146.13 Section 146.13 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.13 Customs forms and...

  12. 40 CFR 146.6 - Area of review.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false Area of review. 146.6 Section 146.6 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS General Provisions § 146.6 Area of review. The area of review for each injection well or...

  13. 19 CFR 146.93 - Inventory control and recordkeeping system.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Inventory control and recordkeeping system. 146.93 Section 146.93 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade Subzones § 146.93 Inventory control and...

  14. 22 CFR 146.100 - Purpose and effective date.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Purpose and effective date. 146.100 Section 146.100 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 146.100 Purpose and effective date. The purpose of these Title...

  15. 22 CFR 146.140 - Dissemination of policy.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Dissemination of policy. 146.140 Section 146.140 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 146.140 Dissemination of policy. (a) Notification of policy. (1)...

  16. 22 CFR 146.600 - Notice of covered programs.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Notice of covered programs. 146.600 Section 146.600 Foreign Relations DEPARTMENT OF STATE CIVIL RIGHTS NONDISCRIMINATION ON THE BASIS OF SEX IN EDUCATION PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Procedures § 146.600 Notice of covered programs. Within 60 days of September...

  17. 19 CFR 146.93 - Inventory control and recordkeeping system.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Inventory control and recordkeeping system. 146.93... § 146.93 Inventory control and recordkeeping system. (a) Attribution. All final products removed from or... provided for under § 146.95(b) of this subpart. (3) Other inventory method. An operator may use the...

  18. 19 CFR 146.63 - Entry for consumption.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Entry for consumption. 146.63 Section 146.63... TREASURY (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.63 Entry for consumption... status may be entered for consumption from a zone. (b) Zone-restricted merchandise. Merchandise in a...

  19. 19 CFR 146.63 - Entry for consumption.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Entry for consumption. 146.63 Section 146.63... TREASURY (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.63 Entry for consumption... status may be entered for consumption from a zone. (b) Zone-restricted merchandise. Merchandise in a...

  20. 19 CFR 146.63 - Entry for consumption.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Entry for consumption. 146.63 Section 146.63... TREASURY (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.63 Entry for consumption... status may be entered for consumption from a zone. (b) Zone-restricted merchandise. Merchandise in a...

  1. 40 CFR 146.4 - Criteria for exempted aquifers.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Criteria for exempted aquifers. 146.4 Section 146.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS General Provisions § 146.4 Criteria for exempted aquifers. An aquifer or...

  2. 40 CFR 146.4 - Criteria for exempted aquifers.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false Criteria for exempted aquifers. 146.4 Section 146.4 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS General Provisions § 146.4 Criteria for exempted aquifers. An aquifer or...

  3. 40 CFR 146.72 - Post-closure care.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false Post-closure care. 146.72 Section 146.72 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED... Hazardous Waste Injection Wells § 146.72 Post-closure care. (a) The owner or operator of a Class I...

  4. 27 CFR 20.146 - Labels on bulk containers.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2014-04-01 2014-04-01 false Labels on bulk containers. 20.146 Section 20.146 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL DISTRIBUTION AND USE OF DENATURED ALCOHOL AND RUM Sale and Use of Completely Denatured Alcohol § 20.146...

  5. 27 CFR 20.146 - Labels on bulk containers.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2013-04-01 2013-04-01 false Labels on bulk containers. 20.146 Section 20.146 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY ALCOHOL DISTRIBUTION AND USE OF DENATURED ALCOHOL AND RUM Sale and Use of Completely Denatured Alcohol § 20.146...

  6. 27 CFR 20.146 - Labels on bulk containers.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2012-04-01 2012-04-01 false Labels on bulk containers. 20.146 Section 20.146 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS DISTRIBUTION AND USE OF DENATURED ALCOHOL AND RUM Sale and Use of Completely Denatured Alcohol § 20.146...

  7. 27 CFR 20.146 - Labels on bulk containers.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2011-04-01 2011-04-01 false Labels on bulk containers. 20.146 Section 20.146 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS DISTRIBUTION AND USE OF DENATURED ALCOHOL AND RUM Sale and Use of Completely Denatured Alcohol § 20.146...

  8. 27 CFR 20.146 - Labels on bulk containers.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Labels on bulk containers. 20.146 Section 20.146 Alcohol, Tobacco Products and Firearms ALCOHOL AND TOBACCO TAX AND TRADE BUREAU, DEPARTMENT OF THE TREASURY LIQUORS DISTRIBUTION AND USE OF DENATURED ALCOHOL AND RUM Sale and Use of Completely Denatured Alcohol § 20.146...

  9. 21 CFR 146.151 - Orange juice for manufacturing.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Orange juice for manufacturing. 146.151 Section... Fruit Juices and Beverages § 146.151 Orange juice for manufacturing. (a) Orange juice for manufacturing... from oranges as provided in § 146.135, except that the oranges may deviate from the standards...

  10. 21 CFR 146.151 - Orange juice for manufacturing.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Orange juice for manufacturing. 146.151 Section... Fruit Juices and Beverages § 146.151 Orange juice for manufacturing. (a) Orange juice for manufacturing... from oranges as provided in § 146.135, except that the oranges may deviate from the standards...

  11. 21 CFR 146.152 - Orange juice with preservative.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Orange juice with preservative. 146.152 Section... Fruit Juices and Beverages § 146.152 Orange juice with preservative. (a) Orange juice with preservative... of orange juice for manufacturing as provided for in § 146.151, except that a preservative is...

  12. 21 CFR 146.152 - Orange juice with preservative.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Orange juice with preservative. 146.152 Section... Fruit Juices and Beverages § 146.152 Orange juice with preservative. (a) Orange juice with preservative... of orange juice for manufacturing as provided for in § 146.151, except that a preservative is...

  13. 21 CFR 146.151 - Orange juice for manufacturing.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Orange juice for manufacturing. 146.151 Section... Fruit Juices and Beverages § 146.151 Orange juice for manufacturing. (a) Orange juice for manufacturing... from oranges as provided in § 146.135, except that the oranges may deviate from the standards...

  14. 21 CFR 146.152 - Orange juice with preservative.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice with preservative. 146.152 Section... Fruit Juices and Beverages § 146.152 Orange juice with preservative. (a) Orange juice with preservative... of orange juice for manufacturing as provided for in § 146.151, except that a preservative is...

  15. 21 CFR 146.151 - Orange juice for manufacturing.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Orange juice for manufacturing. 146.151 Section... Fruit Juices and Beverages § 146.151 Orange juice for manufacturing. (a) Orange juice for manufacturing... from oranges as provided in § 146.135, except that the oranges may deviate from the standards...

  16. 21 CFR 146.151 - Orange juice for manufacturing.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Orange juice for manufacturing. 146.151 Section... Fruit Juices and Beverages § 146.151 Orange juice for manufacturing. (a) Orange juice for manufacturing... from oranges as provided in § 146.135, except that the oranges may deviate from the standards...

  17. 21 CFR 146.152 - Orange juice with preservative.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Orange juice with preservative. 146.152 Section... Fruit Juices and Beverages § 146.152 Orange juice with preservative. (a) Orange juice with preservative... of orange juice for manufacturing as provided for in § 146.151, except that a preservative is...

  18. 21 CFR 146.152 - Orange juice with preservative.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Orange juice with preservative. 146.152 Section... Fruit Juices and Beverages § 146.152 Orange juice with preservative. (a) Orange juice with preservative... of orange juice for manufacturing as provided for in § 146.151, except that a preservative is...

  19. 17 CFR 146.7 - Content of systems of records.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 17 Commodity and Securities Exchanges 1 2013-04-01 2013-04-01 false Content of systems of records. 146.7 Section 146.7 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS § 146.7 Content of systems of records. (a) The Commission will maintain in...

  20. 17 CFR 146.7 - Content of systems of records.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 17 Commodity and Securities Exchanges 1 2011-04-01 2011-04-01 false Content of systems of records. 146.7 Section 146.7 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS § 146.7 Content of systems of records. (a) The Commission will maintain in...

  1. 17 CFR 146.7 - Content of systems of records.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 17 Commodity and Securities Exchanges 1 2012-04-01 2012-04-01 false Content of systems of records. 146.7 Section 146.7 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS § 146.7 Content of systems of records. (a) The Commission will maintain in...

  2. 17 CFR 146.7 - Content of systems of records.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 17 Commodity and Securities Exchanges 2 2014-04-01 2014-04-01 false Content of systems of records. 146.7 Section 146.7 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION (CONTINUED) RECORDS MAINTAINED ON INDIVIDUALS § 146.7 Content of systems of records. (a) The Commission will...

  3. 17 CFR 146.7 - Content of systems of records.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Content of systems of records. 146.7 Section 146.7 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS § 146.7 Content of systems of records. (a) The Commission will maintain in...

  4. 42 CFR 410.146 - Diabetes outcome measurements.

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 42 Public Health 2 2014-10-01 2014-10-01 false Diabetes outcome measurements. 410.146 Section 410.146 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES... Training and Diabetes Outcome Measurements § 410.146 Diabetes outcome measurements. (a)...

  5. 33 CFR 146.120 - Manning of survival craft.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Manning of survival craft. 146.120 Section 146.120 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.120 Manning of survival craft. The owner, the owner's agent, or...

  6. 33 CFR 146.120 - Manning of survival craft.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Manning of survival craft. 146.120 Section 146.120 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.120 Manning of survival craft. The owner, the owner's agent, or...

  7. 33 CFR 146.120 - Manning of survival craft.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Manning of survival craft. 146.120 Section 146.120 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.120 Manning of survival craft. The owner, the owner's agent, or...

  8. 33 CFR 146.120 - Manning of survival craft.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Manning of survival craft. 146.120 Section 146.120 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.120 Manning of survival craft. The owner, the owner's agent, or...

  9. 33 CFR 146.120 - Manning of survival craft.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Manning of survival craft. 146.120 Section 146.120 Navigation and Navigable Waters COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OUTER CONTINENTAL SHELF ACTIVITIES OPERATIONS Manned OCS Facilities § 146.120 Manning of survival craft. The owner, the owner's agent, or...

  10. 7 CFR 762.146 - Other servicing procedures.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 7 2014-01-01 2014-01-01 false Other servicing procedures. 762.146 Section 762.146 Agriculture Regulations of the Department of Agriculture (Continued) FARM SERVICE AGENCY, DEPARTMENT OF AGRICULTURE SPECIAL PROGRAMS GUARANTEED FARM LOANS § 762.146 Other servicing procedures. (a) Additional...

  11. 19 CFR 146.51 - Customs control of merchandise.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Customs control of merchandise. 146.51 Section 146.51 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Handling of Merchandise in a Zone § 146.51...

  12. 19 CFR 146.51 - Customs control of merchandise.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Customs control of merchandise. 146.51 Section 146.51 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Handling of Merchandise in a Zone § 146.51...

  13. 19 CFR 146.39 - Direct delivery procedures.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Direct delivery procedures. 146.39 Section 146.39 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Admission of Merchandise to a Zone § 146.39 Direct...

  14. 19 CFR 146.39 - Direct delivery procedures.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Direct delivery procedures. 146.39 Section 146.39 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Admission of Merchandise to a Zone § 146.39 Direct...

  15. 19 CFR 146.39 - Direct delivery procedures.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Direct delivery procedures. 146.39 Section 146.39 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Admission of Merchandise to a Zone § 146.39 Direct...

  16. 19 CFR 146.39 - Direct delivery procedures.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Direct delivery procedures. 146.39 Section 146.39 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Admission of Merchandise to a Zone § 146.39 Direct...

  17. 19 CFR 146.39 - Direct delivery procedures.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Direct delivery procedures. 146.39 Section 146.39 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Admission of Merchandise to a Zone § 146.39 Direct...

  18. 15 CFR 14.6 - Availability of OMB circulars.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 15 Commerce and Foreign Trade 1 2010-01-01 2010-01-01 false Availability of OMB circulars. 14.6 Section 14.6 Commerce and Foreign Trade Office of the Secretary of Commerce UNIFORM ADMINISTRATIVE... COMMERCIAL ORGANIZATIONS General § 14.6 Availability of OMB circulars. OMB circulars cited in this part...

  19. 15 CFR 14.6 - Availability of OMB circulars.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 15 Commerce and Foreign Trade 1 2011-01-01 2011-01-01 false Availability of OMB circulars. 14.6 Section 14.6 Commerce and Foreign Trade Office of the Secretary of Commerce UNIFORM ADMINISTRATIVE... COMMERCIAL ORGANIZATIONS General § 14.6 Availability of OMB circulars. OMB circulars cited in this part...

  20. 19 CFR 146.63 - Entry for consumption.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Entry for consumption. 146.63 Section 146.63... TREASURY (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.63 Entry for consumption... status may be entered for consumption from a zone. (b) Zone-restricted merchandise. Merchandise in a...

  1. 19 CFR 146.63 - Entry for consumption.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry for consumption. 146.63 Section 146.63... TREASURY (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.63 Entry for consumption... status may be entered for consumption from a zone. (b) Zone-restricted merchandise. Merchandise in a...

  2. 34 CFR 300.146 - Responsibility of SEA.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Responsibility of SEA. 300.146 Section 300.146... Referred by Public Agencies § 300.146 Responsibility of SEA. Each SEA must ensure that a child with a... the standards that apply to education provided by the SEA and LEAs including the requirements of...

  3. 7 CFR 1703.146 - Submission of applications.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 11 2013-01-01 2013-01-01 false Submission of applications. 1703.146 Section 1703.146 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan Program § 1703.146 Submission...

  4. 17 CFR 146.13 - Inspector General exemptions.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 17 Commodity and Securities Exchanges 1 2010-04-01 2010-04-01 false Inspector General exemptions. 146.13 Section 146.13 Commodity and Securities Exchanges COMMODITY FUTURES TRADING COMMISSION RECORDS MAINTAINED ON INDIVIDUALS § 146.13 Inspector General exemptions. (a) Pursuant to section (j) of the Privacy Act of 1974, the Commission has...

  5. 42 CFR 433.146 - Rights assigned; assignment method.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 42 Public Health 4 2010-10-01 2010-10-01 false Rights assigned; assignment method. 433.146 Section 433.146 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN... Assignment of Rights to Benefits § 433.146 Rights assigned; assignment method. (a) Except as specified...

  6. 19 CFR 146.53 - Shortages and overages.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Shortages and overages. 146.53 Section 146.53 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Handling of Merchandise in a Zone § 146.53 Shortages and overages. (a) Report required. The operator...

  7. 40 CFR 146.72 - Post-closure care.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 22 2010-07-01 2010-07-01 false Post-closure care. 146.72 Section 146... Hazardous Waste Injection Wells § 146.72 Post-closure care. (a) The owner or operator of a Class I hazardous waste well shall prepare, maintain, and comply with a plan for post-closure care that meets...

  8. 40 CFR 146.72 - Post-closure care.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 24 2012-07-01 2012-07-01 false Post-closure care. 146.72 Section 146... Hazardous Waste Injection Wells § 146.72 Post-closure care. (a) The owner or operator of a Class I hazardous waste well shall prepare, maintain, and comply with a plan for post-closure care that meets...

  9. 40 CFR 146.72 - Post-closure care.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 23 2011-07-01 2011-07-01 false Post-closure care. 146.72 Section 146... Hazardous Waste Injection Wells § 146.72 Post-closure care. (a) The owner or operator of a Class I hazardous waste well shall prepare, maintain, and comply with a plan for post-closure care that meets...

  10. 40 CFR 146.72 - Post-closure care.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 24 2013-07-01 2013-07-01 false Post-closure care. 146.72 Section 146... Hazardous Waste Injection Wells § 146.72 Post-closure care. (a) The owner or operator of a Class I hazardous waste well shall prepare, maintain, and comply with a plan for post-closure care that meets...

  11. 13 CFR 146.500 - Secretary of Defense.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 13 Business Credit and Assistance 1 2014-01-01 2014-01-01 false Secretary of Defense. 146.500 Section 146.500 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW RESTRICTIONS ON LOBBYING Exemptions § 146.500 Secretary of Defense. (a) The Secretary of Defense may exempt, on a case-by-case basis, a covered Federal action from...

  12. 7 CFR 1703.146 - Submission of applications.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 11 2011-01-01 2011-01-01 false Submission of applications. 1703.146 Section 1703.146 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE RURAL DEVELOPMENT Distance Learning and Telemedicine Loan Program § 1703.146 Submission of applications. (a) RUS will...

  13. 40 CFR 146.9 - Criteria for establishing permitting priorities.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 23 2014-07-01 2014-07-01 false Criteria for establishing permitting priorities. 146.9 Section 146.9 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) UNDERGROUND INJECTION CONTROL PROGRAM: CRITERIA AND STANDARDS General Provisions § 146.9 Criteria for...

  14. 45 CFR 146.143 - Preemption; State flexibility; construction.

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 45 Public Welfare 1 2011-10-01 2011-10-01 false Preemption; State flexibility; construction. 146.143 Section 146.143 Public Welfare DEPARTMENT OF HEALTH AND HUMAN SERVICES REQUIREMENTS RELATING TO HEALTH CARE ACCESS REQUIREMENTS FOR THE GROUP HEALTH INSURANCE MARKET Preemption and Special Rules § 146.143 Preemption; State...

  15. 19 CFR 146.14 - Retail trade within a zone.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... the Board as contained in 15 CFR part 400. ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Retail trade within a zone. 146.14 Section 146.14... TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.14 Retail trade within a zone....

  16. 19 CFR 146.14 - Retail trade within a zone.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... the Board as contained in 15 CFR part 400. ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Retail trade within a zone. 146.14 Section 146.14... TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.14 Retail trade within a zone....

  17. 19 CFR 146.14 - Retail trade within a zone.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... the Board as contained in 15 CFR part 400. ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Retail trade within a zone. 146.14 Section 146.14... TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.14 Retail trade within a zone....

  18. 19 CFR 146.14 - Retail trade within a zone.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... the Board as contained in 15 CFR part 400. ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Retail trade within a zone. 146.14 Section 146.14... TREASURY (CONTINUED) FOREIGN TRADE ZONES General Provisions § 146.14 Retail trade within a zone....

  19. 5 CFR 185.146 - Compromise or settlement.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 5 Administrative Personnel 1 2010-01-01 2010-01-01 false Compromise or settlement. 185.146 Section 185.146 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS PROGRAM FRAUD CIVIL REMEDIES § 185.146 Compromise or settlement. (a) Parties may make offers of compromise...

  20. 17 CFR 256.146 - Accounts receivable from associate companies.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... associate companies. 256.146 Section 256.146 Commodity and Securities Exchanges SECURITIES AND EXCHANGE COMMISSION (CONTINUED) UNIFORM SYSTEM OF ACCOUNTS FOR MUTUAL SERVICE COMPANIES AND SUBSIDIARY SERVICE COMPANIES, PUBLIC UTILITY HOLDING COMPANY ACT OF 1935 3. Current and Accrued Assets § 256.146...

  1. 19 CFR 146.64 - Entry for warehouse.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Entry for warehouse. 146.64 Section 146.64 Customs... (CONTINUED) FOREIGN TRADE ZONES Transfer of Merchandise From a Zone § 146.64 Entry for warehouse. (a) Foreign... status may not be entered for warehouse from a zone. Merchandise in nonprivileged foreign...

  2. 9 CFR 146.8 - Terminology and classification; slaughter plants.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 9 Animals and Animal Products 1 2010-01-01 2010-01-01 false Terminology and classification; slaughter plants. 146.8 Section 146.8 Animals and Animal Products ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE LIVESTOCK IMPROVEMENT NATIONAL POULTRY IMPROVEMENT PLAN FOR COMMERCIAL POULTRY General Provisions § 146.8...

  3. Near-infrared electroluminescence at room temperature from neodymium-doped gallium nitride thin films

    SciTech Connect

    Kim, Joo Han; Holloway, Paul H.

    2004-09-06

    Strong near-infrared (NIR) electroluminescence (EL) at room temperature from neodymium (Nd)-doped gallium nitride (GaN) thin films is reported. The Nd-doped GaN films were grown by radio-frequency planar magnetron cosputtering of separate GaN and metallic Nd targets in a pure nitrogen ambient. X-ray diffraction data did not identify the presence of any secondary phases and revealed that the Nd-doped GaN films had a highly textured wurtzite crystal structure with the c-axis normal to the surface of the film. The EL devices were fabricated with a thin-film multilayered structure of Al/Nd-doped GaN/Al{sub 2}O{sub 3}-TiO{sub 2}/indium-tin oxide and tested at room temperate. Three distinct NIR EL emission peaks were observed from the devices at 905, 1082, and 1364 nm, arising from the radiative relaxation of the {sup 4}F{sub 3sol2} excited-state energy level to the {sup 4}I{sub 9sol2}, {sup 4}I{sub 11sol2}, and {sup 4}I{sub 13sol2} levels of the Nd{sup 3+} ion, respectively. The threshold voltage for all the three emission peaks was {approx}150 V. The external power efficiency of the fabricated EL devices was {approx}1x10{sup -5} measured at 40 V above the threshold voltage.

  4. Roquin binds microRNA-146a and Argonaute2 to regulate microRNA homeostasis

    PubMed Central

    Srivastava, Monika; Duan, Guowen; Kershaw, Nadia J.; Athanasopoulos, Vicki; Yeo, Janet H. C.; Ose, Toyoyuki; Hu, Desheng; Brown, Simon H. J.; Jergic, Slobodan; Patel, Hardip R.; Pratama, Alvin; Richards, Sashika; Verma, Anil; Jones, E. Yvonne; Heissmeyer, Vigo; Preiss, Thomas; Dixon, Nicholas E.; Chong, Mark M. W.; Babon, Jeffrey J.; Vinuesa, Carola G.

    2015-01-01

    Roquin is an RNA-binding protein that prevents autoimmunity and inflammation via repression of bound target mRNAs such as inducible costimulator (Icos). When Roquin is absent or mutated (Roquinsan), Icos is overexpressed in T cells. Here we show that Roquin enhances Dicer-mediated processing of pre-miR-146a. Roquin also directly binds Argonaute2, a central component of the RNA-induced silencing complex, and miR-146a, a microRNA that targets Icos mRNA. In the absence of functional Roquin, miR-146a accumulates in T cells. Its accumulation is not due to increased transcription or processing, rather due to enhanced stability of mature miR-146a. This is associated with decreased 3′ end uridylation of the miRNA. Crystallographic studies reveal that Roquin contains a unique HEPN domain and identify the structural basis of the ‘san’ mutation and Roquin’s ability to bind multiple RNAs. Roquin emerges as a protein that can bind Ago2, miRNAs and target mRNAs, to control homeostasis of both RNA species. PMID:25697406

  5. 33 CFR 146.20 - Work vests.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... CFR 160.053; or (2) 46 CFR 160.077 as a commercial hybrid PFD. (b) Use. Approved buoyant work vests... by 46 CFR 160.077-29; and (ii) Any limitation(s) marked on them; and (2) Of the same or similar... 33 Navigation and Navigable Waters 2 2012-07-01 2012-07-01 false Work vests. 146.20 Section...

  6. 33 CFR 146.20 - Work vests.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... CFR 160.053; or (2) 46 CFR 160.077 as a commercial hybrid PFD. (b) Use. Approved buoyant work vests... by 46 CFR 160.077-29; and (ii) Any limitation(s) marked on them; and (2) Of the same or similar... 33 Navigation and Navigable Waters 2 2013-07-01 2013-07-01 false Work vests. 146.20 Section...

  7. 33 CFR 146.20 - Work vests.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... CFR 160.053; or (2) 46 CFR 160.077 as a commercial hybrid PFD. (b) Use. Approved buoyant work vests... by 46 CFR 160.077-29; and (ii) Any limitation(s) marked on them; and (2) Of the same or similar... 33 Navigation and Navigable Waters 2 2011-07-01 2011-07-01 false Work vests. 146.20 Section...

  8. 33 CFR 146.20 - Work vests.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... CFR 160.053; or (2) 46 CFR 160.077 as a commercial hybrid PFD. (b) Use. Approved buoyant work vests... by 46 CFR 160.077-29; and (ii) Any limitation(s) marked on them; and (2) Of the same or similar... 33 Navigation and Navigable Waters 2 2014-07-01 2014-07-01 false Work vests. 146.20 Section...

  9. 33 CFR 146.20 - Work vests.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... CFR 160.053; or (2) 46 CFR 160.077 as a commercial hybrid PFD. (b) Use. Approved buoyant work vests... by 46 CFR 160.077-29; and (ii) Any limitation(s) marked on them; and (2) Of the same or similar... 33 Navigation and Navigable Waters 2 2010-07-01 2010-07-01 false Work vests. 146.20 Section...

  10. FOXP3-microRNA-146-NF-κB axis and therapy for precancerous lesions in prostate

    PubMed Central

    Liu, Runhua; Yi, Bin; Wei, Shi; Yang, Wei-Hsiung; Hart, Karen M.; Chauhan, Priyanka; Zhang, Wei; Mao, Xicheng; Liu, Xiuping; Liu, Chang-Gong; Wang, Lizhong

    2015-01-01

    The tumor suppressive activity of FOXP3 has been observed in tumor initiation, but the underlying mechanism still remains largely unknown. Here, we identified a FOXP3-microRNA-146 (miR-146)-NF-κB axis in vitro and in vivo in prostate cancer cells. We observed that FOXP3 dramatically induced the expression of miR-146a/b, which contributed to transcriptional inhibition of IRAK1 and TRAF6, in prostate cancer cell lines. Tissue-specific deletion of Foxp3 in mouse prostate caused a significant reduction of miR-146a and upregulation of NF-κB activation. In addition, prostatic intraepithelial neoplasia lesions were observed in miR-146a mutant mice as well as in Foxp3 mutant mice. Notably, the NF-κB inhibitor bortezomib inhibited cell proliferation and induced apoptosis in prostate epithelial cells, attenuating prostatic intraepithelial neoplasia formation in Foxp3 mutant mice. Our data suggest that the FOXP3-miR-146-NF-κB axis has a functional role during tumor initiation in prostate cancer. Targeting the miR-146-NF-κB axis may provide a new therapeutic approach for prostate cancers with FOXP3 defects. PMID:25712341

  11. The role of microRNA-146a in the pathogenesis of the diabetic wound-healing impairment: correction with mesenchymal stem cell treatment.

    PubMed

    Xu, Junwang; Wu, Wenjie; Zhang, Liping; Dorset-Martin, Wanda; Morris, Michael W; Mitchell, Marc E; Liechty, Kenneth W

    2012-11-01

    The impairment in diabetic wound healing represents a significant clinical problem. Chronic inflammation is thought to play a central role in the pathogenesis of this impairment. We have previously shown that treatment of diabetic murine wounds with mesenchymal stem cells (MSCs) can improve healing, but the mechanisms are not completely defined. MicroRNA-146a (miR-146a) has been implicated in regulation of the immune and inflammatory responses. We hypothesized that abnormal miRNA-146a expression may contribute to the chronic inflammation. To test this hypothesis, we examined the expression of miRNA-146a and its target genes in diabetic and nondiabetic mice at baseline and after injury. MiR-146a expression was significantly downregulated in diabetic mouse wounds. Decreased miR-146a levels also closely correlated with increased gene expression of its proinflammatory target genes. Furthermore, the correction of the diabetic wound-healing impairment with MSC treatment was associated with a significant increase in the miR-146a expression level and decreased gene expression of its proinflammatory target genes. These results provide the first evidence that decreased expression of miR-146a in diabetic wounds in response to injury may, in part, be responsible for the abnormal inflammatory response seen in diabetic wounds and may contribute to wound-healing impairment. PMID:22851573

  12. Identification of a novel miR-146a from Pinctada martensii involved in the regulation of the inflammatory response.

    PubMed

    Zheng, Zhe; Liang, Jinlian; Huang, Ronglian; Du, Xiaodong; Wang, Qingheng; Deng, Yuewen; Jiao, Yu

    2016-07-01

    Increasing evidence demonstrated that microRNAs (miRNAs) play critical roles in innate immunity in vertebrates and invertebrates. MiR-146a/b is reported as a key regulator of the immune response through mediating Toll-like receptor and cytokine signalling. In this study, a novel miR-146a was identified and characterised from Pinctada martensii (designated as pm-miR-146a), and its roles in modulating the inflammatory response after LPS stimulation were also investigated. Pm-miR-146a ubiquitously expressed in all examined tissues, with the highest level in the mantle and lowest expression in the haemolymph. Pm-miR-146a increased at 24 h after lipoplysaccharide injection, in union with up-regulated NF-κB (P < 0.05). The over-expression of pm-miR-146a in vivo could significantly inhibit the expression of macrophage migration inhibitory factor (MIF), the potential target gene predicted by miRanda, while enforcing pm-miR-146a involved in the down-regulation of NF-κB. Thus, we propose that pm-miR-146a plays a role of negative feedback regulation to the NF-κB signal by repressing the expression of the pro-inflammatory cytokine MIF. These findings revealed that miR-146a represents a critical role in inflammatory response and offers new evidence for miRNAs in the innate immunity of molluscs. PMID:26993611

  13. MicroRNA-146a regulates ICOS–ICOSL signalling to limit accumulation of T follicular helper cells and germinal centres

    PubMed Central

    Pratama, Alvin; Srivastava, Monika; Williams, Naomi J.; Papa, Ilenia; Lee, Sau K.; Dinh, Xuyen T.; Hutloff, Andreas; Jordan, Margaret A.; Zhao, Jimmy L.; Casellas, Rafael; Athanasopoulos, Vicki; Vinuesa, Carola G.

    2015-01-01

    Tight control of T follicular helper (Tfh) cells is required for optimal maturation of the germinal centre (GC) response. The molecular mechanisms controlling Tfh-cell differentiation remain incompletely understood. Here we show that microRNA-146a (miR-146a) is highly expressed in Tfh cells and peak miR-146a expression marks the decline of the Tfh response after immunization. Loss of miR-146a causes cell-intrinsic accumulation of Tfh and GC B cells. MiR-146a represses several Tfh-cell-expressed messenger RNAs, and of these, ICOS is the most strongly cell autonomously upregulated target in miR-146a-deficient T cells. In addition, miR-146a deficiency leads to increased ICOSL expression on GC B cells and antigen-presenting cells. Partial blockade of ICOS signalling, either by injections of low dose of ICOSL blocking antibody or by halving the gene dose of Icos in miR-146a-deficient T cells, prevents the Tfh and GC B-cell accumulation. Collectively, miR-146a emerges as a post-transcriptional brake to limit Tfh cells and GC responses. PMID:25743066

  14. Effect Of Neodymium Substitution In Structural Characteristics Of Magnesium Ferrite

    SciTech Connect

    Thankachan, Smitha; Binu, P. J.; Xavier, Sheena; Mohammed, E. M.

    2011-10-20

    The effect of Nd{sup 3+} substitution on the structural properties of Magnesium ferrite was studied in the series MgNd{sub x}Fe{sub 2-x}O{sub 4}, where x = 0 to 0.3 in steps of 0.05. The series was prepared by sol-gel technique which is one of the novel technique to prepare nanosized samples. Structural characterization was done using X-ray diffractometer and Fourier Transform Infrared Spectrometer. XRD analysis reveals the prepared samples are single phasic till x = 0.2. From x0 = .25, a secondary phase of iron neodymium oxide appears along with the spinel phase. Particle size calculation shows the prepared samples are in the 9nm to 11 nm regime. Lattice parameter was found to increase with concentration of Nd. XRD and FTIR analysis confirmed spinel structure of the prepared samples. XRF result shows the expected composition of prepared samples. The frequency dependence of the dielectric constant in the range 100 Hz--120MHz was also studied

  15. Power neodymium-glass amplifier of a repetitively pulsed laser

    SciTech Connect

    Vinogradov, Aleksandr V; Gaganov, V E; Garanin, Sergey G; Zhidkov, N V; Krotov, V A; Martynenko, S P; Pozdnyakov, E V; Solomatin, I I

    2011-11-30

    A neodymium-glass diode-pumped amplifier with a zigzag laser beam propagation through the active medium was elaborated; the amplifier is intended for operation in a repetitively pulsed laser. An amplifier unit with an aperture of 20 Multiplication-Sign 25 mm and a {approx}40-cm long active medium was put to a test. The energy of pump radiation amounts to 140 J at a wavelength of 806 nm for a pump duration of 550 {mu}s. The energy parameters of the amplifier were experimentally determined: the small-signal gain per pass {approx}3.2, the linear gain {approx}0.031 cm{sup -1} with a nonuniformity of its distribution over the aperture within 15%, the stored energy of 0.16 - 0.21 J cm{sup -3}. The wavefront distortions in the zigzag laser-beam propagation through the active element of the amplifier did not exceed 0.4{lambda} ({lambda} = 0.63 {mu}m is the probing radiation wavelength).

  16. Laser self-doubling in neodymium yttrium aluminum borate

    NASA Astrophysics Data System (ADS)

    Lu, Bao-Sheng; Wang, Jun; Pan, Heng-Fu; Jiang, Min-Hua; Liu, En-Quan

    1989-12-01

    The nonlinear effects of neodymium yttrium aluminum borate Nd(x)Y(1-x)Al3(BO3)4 (NYAB) crystal powder samples with different Nd(3+) mole percentages are reported. The optimum X values for the growth of NYAB crystals with high optical homogeneity has been determined. The performance of laser self-frequency doubling from 1.06-0.53 micron has been realized, for the first time, in a 5 x 3 x 3 cu mm NYAB crystal. The Nd(3+) ions in crystal show weak absorption at 0.53 micron. The threshold energy is measured to be less than 2 mJ; the output energy of green light at 0.53 micron is more than 5 mJ and the conversion efficiency is over 10 percent. The experiments show that the performance is improved if the optical path length is increased and the cavity design is improved. The refractive indices n(0) and n(e) of the crystal have been measured by the prism method at different wavelengths. The phase-matching angles of Types I and II have been obtained by solving equations for the phase-matching angles. The results are in good agreement with the experimental values. The nonlinear coefficient d(11) of the NYAB crystal has been measured to be 4 x 10 to the-9th esu.

  17. LASERS: Efficient neodymium-doped gadolinium gallium garnet crystal laser

    NASA Astrophysics Data System (ADS)

    Doroshenko, Maxim E.; Osiko, Vyacheslav V.; Sigachev, V. B.; Timoshechkin, M. I.

    1991-07-01

    An investigation was made of the stimulated emission parameters of a laser utilizing a gadolinium gallium garnet crystal doped with neodymium ions (YAG:Nd) at the 1.062 μm wavelength. The free-running efficiency was the highest so far achieved for flashlamp-pumped lasers utilizing unsensitized garnets. For an active element 8 mm in diameter and 120 mm long the absolute efficiency was 5.4% and the differential efficiency was 5.9%. The average free-running power was 170 W. A comparison was made of the optical powers of thermal lenses in cylindrical GGG:Nd and YAG:Nd active elements and this was found to be 2.4 times higher for a GGG:Nd crystal at the same pump powers. It was shown that by using traditional methods of compensating for the thermal lens in cylindrical active elements, it is possible to develop pulsed GGG:Nd crystal lasers having an average output power higher than 100 W, an efficiency of ~ 4%, and an angular divergence of less than 10 mrad.

  18. Dielectric and impedance spectroscopic studies of neodymium gallate

    NASA Astrophysics Data System (ADS)

    Sakhya, Anup Pradhan; Dutta, Alo; Sinha, T. P.

    2016-05-01

    The AC electrical properties of a polycrystalline neodymium gallate, NdGaO3 (NGO), synthesized by the sol-gel method have been investigated by employing impedance spectroscopy in the frequency range from 42 Hz to 5 MHz and in the temperature range from 323 K to 593 K. The X-ray diffraction analysis shows that the compound crystallizes in the orthorhombic phase with Pbnm space group at room temperature. Two relaxation processes with different relaxation times are observed from the impedance as well as modulus spectroscopic measurements, which have been attributed to the grain and the grain boundary effects at different temperatures in NGO. The complex impedance data are analyzed by an electrical equivalent circuit consisting of a resistance and a constant phase element in parallel. It has been observed that the value of the capacitance and the resistance associated with the grain boundary is higher than those associated with the grain. The temperature dependent electrical conductivity shows the negative temperature coefficient of resistance. The frequency dependent conductivity spectra are found to follow the power law.

  19. Laboratory and clinical experience with neodymium:YAG laser prostatectomy

    NASA Astrophysics Data System (ADS)

    Kabalin, John N.

    1996-05-01

    Since 1991, we have undertaken extensive laboratory and clinical studies of the Neodymium:YAG (Nd:YAG) laser for surgical treatment of bladder outlet obstruction due to prostatic enlargement or benign prostatic hyperplasia (BPH). Side-firing optical fibers which emit a divergent, relatively low energy density Nd:YAG laser beam produce coagulation necrosis of obstructing periurethral prostate tissue, followed by gradual dissolution and slough in the urinary stream. Laser-tissue interactions and Nd:YAG laser dosimetry for prostatectomy have been studied in canine and human prostate model systems, enhancing clinical application. Ongoing studies examine comparative Nd:YAG laser dosimetry for various beam configurations produced by available side-firing optical fibers and continue to refine operative technique. We have documented clinical outcomes of Nd:YAG laser prostatectomy in 230 consecutive patients treated with the UrolaseTM side-firing optical fiber. Nd:YAG laser coagulation the prostate produces a remarkably low acute morbidity profile, with no significant bleeding or fluid absorption. No postoperative incontinence has been produced. Serial assessments of voiding outcomes over more than 3 years of followup show objective and symptomatic improvement following Nd:YAG laser prostatectomy which is comparable to older but more morbid electrosurgical approaches. Nd:YAG laser prostatectomy is a safe, efficacious, durable and cost-effective treatment for BPH.

  20. Functional SNP in stem of mir-146a affects Her2 status and breast cancer survival.

    PubMed

    Meshkat, Mahboobeh; Tanha, Hamzeh Mesrian; Naeini, Marjan Mojtabavi; Ghaedi, Kamran; Sanati, Mohammad H; Meshkat, Marzieh; Bagheri, Fatemeh

    2016-07-01

    In-silico investigation suggested a common variant within stem of miR-146a-5p precursor (rs2910164, n.60C>G) associated with breast cancer (BC) phenotypes. Our aim was computationally predicting possible targets of miR-146a-5p and probable rs2910164 mechanism of action in expression of phenotypes in BC. Additionally, a case-control study was designated to examine experimentally the correlation of mir-146a rs2910164 variant and BC phenotypes. In this study, 152 BC subjects and healthy controls were genotyped using RFLP-PCR. Allelic and genotypic association and Armitage's trend tests were run to investigate the correlation between the alleles and genotypes and expressed phenotypes of BC. Bioinformatics analyses introduce regulatory function of miR-146a-5p in numerous signaling pathways and impact of allele substitution upon mir-146a stem-loop stability. Logistic regression data represented the C allele of rs2910164 (OR = 4.00, p= 0.0037) as the risk allele and associated with Her2-positive phenotype. In a similar vein, data revealed the correlation of the C allele and cancer death less than two years in BC patients (OR = 2.65, p= 0.0217). Ultimately, unconditional logistical regression models suggested log-additive model for inheritance manner of rs2910164 in either Her2 status or BC survival (OR = 5.64, p= 0.0025 and OR = 3.13, p= 0.019, respectively). Using bioinformatics connected association of Her2 status to altered function of miR-146a-5p in regulation of focal adhesion and Ras pathway. Furthermore, computations inferred the association between death phenotype and studied SNP upon specific target genes of miR-146a-5p involved in focal adhesion, EGF receptor, Ras, ErbB, interleukin, Toll-like receptor, NGF, angiogenesis, and p53 feedback loops 2 signaling pathways. These verdicts may enhance our perceptions of how mir-146a rs2910164 affect expressed phenotypes in BC, and might have potential implications to develop BC treatment in future. PMID:27434289

  1. Inhibition of miR-146b expression increases radioiodine-sensitivity in poorly differential thyroid carcinoma via positively regulating NIS expression

    SciTech Connect

    Li, Luchuan; Lv, Bin; Chen, Bo; Guan, Ming; Sun, Yongfeng; Li, Haipeng; Zhang, Binbin; Ding, Changyuan; He, Shan; Zeng, Qingdong

    2015-07-10

    Dedifferentiated thyroid carcinoma (DTC) with the loss of radioiodine uptake (RAIU) is often observed in clinical practice under radioiodine therapy, indicating the challenge for poor prognosis. MicroRNA (miRNA) has emerged as a promising therapeutic target in many diseases; yet, the role of miRNAs in RAIU has not been generally investigated. Based on recent studies about miRNA expression in papillary or follicular thyroid carcinomas, the expression profiles of several thyroid relative miRNAs were investigated in one DTC cell line, derived from normal DTC cells by radioiodine treatment. The top candidate miR-146b, with the most significant overexpression profiles in dedifferentiated cells, was picked up. Further research found that miR-146b could be negatively regulated by histone deacetylase 3 (HDAC3) in normal cells, indicating the correlation between miR-146b and Na{sup +}/I{sup −} symporter (NIS)-mediated RAIU. Fortunately, it was confirmed that miR-146b could regulate NIS expression/activity; what is more important, miR-146b interference would contribute to the recovery of radioiodine-sensitivity in dedifferentiated cells via positively regulating NIS. In the present study, it was concluded that NIS-mediated RAIU could be modulated by miR-146b; accordingly, miR-146b might serve as one of targets to enhance efficacy of radioactive therapy against poorly differential thyroid carcinoma (PDTC). - Highlights: • Significant upregulated miR-146b was picked up from thyroid relative miRNAs in DTC. • MiR-146b was negatively regulated by HDAC3 in normal thyroid carcinoma cells. • NIS activity and expression could be regulated by miR-146b in thyroid carcinoma. • MiR-146b inhibition could recover the decreased radioiodine-sensitivity of DTC cells.

  2. Electrolytic production of neodymium metal from a molten chloride electrolyte. Rept. of Investigations/1991

    SciTech Connect

    Chambers, M.F.; Murphy, J.E.

    1991-01-01

    Electrowinning of neodymium metal was accomplished by using a molten-metal cathode at 650 C and an electrolyte of 50 mol pct neodymium chloride-50 mol pct potassium chloride. The molten-metal cathodes were alloys of magnesium and zinc or magnesium and cadmium. Current efficiencies were 90 pct with a Mg/Zn cathode and 80 pct with a Mg-Cd cathode. The Mg-Cd cathode was easily separated from the electrolyte. In contrast, the Mg-Zn cathode tended to mix with the electrolyte, making separation difficult. The cathode metals were separated from the neodymium by distillation at 1,100 C under a vacuum of 10 to the -3rd power torr. Neodymium metal of 99.9+ purity was recovered from the Mg-Cd alloy cathode after 30 min distillation time. The neodymium recovered from the Mg-Zn system contained almost 2 pct Zn after vacuum distillation. Continuous operation using the Mg-Cd alloy cathode was demonstrated.

  3. Electrolytic production of neodymium without perfluorinated carbon compounds on the offgases

    DOEpatents

    Keller, R.; Larimer, K.T.

    1998-09-22

    A method is described for producing neodymium in an electrolytic cell without formation of perfluorinated carbon gases (PFCs), the method comprising the steps of providing an electrolyte in the electrolytic cell and providing an anode in an anode region of the electrolyte and providing a cathode in a cathode region of the electrolytic cell. Dissolving an oxygen-containing neodymium compound in the electrolyte in the anode region and maintaining a more intense electrolyte circulation in the anode region than in the cathode region. Passing an electrolytic current between said anode and said cathode and depositing neodymium metal at the cathode, preventing the formation of perfluorinated carbon gases by limiting anode over voltage. 4 figs.

  4. Electrowinning of neodymium from a molten oxide-fluoride electrolyte. Report of investigations/1994

    SciTech Connect

    Dysinger, D.K.; Murphy, J.E.

    1994-01-01

    Neodymium metal of 99.8 percent purity was prepared by electrolysis of Nd2O3 salts dissolved in a molten fluoride electrolyte. The metal was electrowon in a molten state at current efficiencies of 50 to 60 percent. Oxygen and carbon were the major impurities detected in the product. During operation of the small scale laboratory cell, a number of technical problems including anode effect, low oxide solubility in the electrolyte, high neodymium metal solubility, reactivity of the metal with the cell materials, and back reaction of the metal with the anode gases were encountered. Approaches to improve cell operation and prospects for commercial adoption of the electrolytic production of neodymium metal are discussed.

  5. Rare earth elements and neodymium isotopes in sedimentary organic matter

    NASA Astrophysics Data System (ADS)

    Freslon, Nicolas; Bayon, Germain; Toucanne, Samuel; Bermell, Sylvain; Bollinger, Claire; Chéron, Sandrine; Etoubleau, Joel; Germain, Yoan; Khripounoff, Alexis; Ponzevera, Emmanuel; Rouget, Marie-Laure

    2014-09-01

    We report rare earth element (REE) and neodymium (Nd) isotope data for the organic fraction of sediments collected from various depositional environments, i.e. rivers (n = 25), estuaries (n = 18), open-ocean settings (n = 15), and cold seeps (n = 12). Sedimentary organic matter (SOM) was extracted using a mixed hydrogen peroxide/nitric acid solution (20%-H2O2-0.02 M-HNO3), after removal of carbonate and oxy-hydroxide phases with dilute hydrochloric acid (0.25 M-HCl). A series of experimental tests indicate that extraction of sedimentary organic compounds using H2O2 may be complicated occasionally by partial dissolution of sulphide minerals and residual carbonates. However, this contamination is expected to be minor for REE because measured concentrations in H2O2 leachates are about two-orders of magnitude higher than in the above mentioned phases. The mean REE concentrations determined in the H2O2 leachates for samples from rivers, estuaries, coastal seas and open-ocean settings yield relatively similar levels, with ΣREE = 109 ± 86 ppm (mean ± s; n = 58). The organic fractions leached from cold seep sediments display even higher concentration levels (285 ± 150 ppm; mean ± s; n = 12). The H2O2 leachates for most sediments exhibit remarkably similar shale-normalized REE patterns, all characterized by a mid-REE enrichment compared to the other REE. This suggests that the distribution of REE in leached sedimentary organic phases is controlled primarily by biogeochemical processes, rather than by the composition of the source from which they derive (e.g. pore, river or sea-water). The Nd isotopic compositions for organic phases leached from river sediments are very similar to those for the corresponding detrital fractions. In contrast, the SOM extracted from marine sediments display εNd values that typically range between the εNd signatures for terrestrial organic matter (inferred from the analysis of the sedimentary detrital fractions) and marine organic matter

  6. Hafnium and Neodymium Isotopes in Atlantic Ocean Waters

    NASA Astrophysics Data System (ADS)

    Rickli, J.; Frank, M.; Halliday, A.

    2007-12-01

    Neodymium isotopic compositions (ICs) have been established as a tracer of water masses in the present and past oceans since the late 1970s. Hafnium isotopes share the capability of tracing water masses and in combination with Nd isotopes provide information on continental weathering regimes. Whereas Nd released during weathering reflects the bulk Nd IC of the weathered lithology, the released Hf is more radiogenic than the weathered lithology. This effect is due to highly variable Lu/Hf--ratios in rock--forming minerals ("zircon effect") and as a consequence physical weathering apparently leads to more congruent weathering of Hf than chemical weathering does. Our understanding of the Hf IC of seawater to date has been derived (with the exception of some as yet unpublished data from the Arctic and Pacific oceans (Zimmermann et al., in prep.)) from ferromanganese crusts and nodules, since Hf concentrations in seawater are low and have until recently hampered direct measurements of Hf IC of seawater. We present IC for the dissolved Hf and Nd in Atlantic seawater. Samples were taken mainly on a transect from the Bay of Biscay to Cape Town (RV Polarstern cruise ANT XXIII/1 in 2005). A few additional samples are from the Labrador Sea and the Drake Passage. Hafnium and Nd were pre--concentrated by iron co--precipitation from 60 to 140 liters of filtered (0.45 μm) seawater. Separation of Hf and Nd followed previously established ion chromatographic procedures. Hafnium and Nd ICs were measured by MC--ICPMS (Nu Plasma) with a 2σ external reproducibility of 0.65 and 0.3 ǎrepsilon--units, respectively. Sample sizes varied but were in most cases larger than 3ng of Hf. Surface seawater as well as deep water samples extending to ~5,000 m, plot on the "seawater array" defined previously from measurements of ferromanganese crusts and nodules. Surface seawater ICs are quite uniform for Hf ranging from ǎrepsilonHf = 0 to +2 at most sampling sites on the Atlantic transect. In the

  7. Recent Progress in the Development of Neodymium Doped Ceramic Yttria

    NASA Technical Reports Server (NTRS)

    Prasad, Narasimha S.; Edwards, Chris; Trivedi, Sudhir B.; Kutcher, Susan; Wang, Chen-Chia; Kim, Joo-Soo; Hommerich, Uwe; Shukla, Vijay; Sadangi, Rajendra; Kear, Bernard

    2007-01-01

    Solid-state lasers play a significant role in providing the technology necessary for active remote sensing of the atmosphere. Neodymium doped yttria (Nd:Y2O3) is considered to be an attractive material due to its possible lasing wavelengths of aprrox.914 nm and approx.946 nm for ozone profiling. These wavelengths when frequency tripled can generate UV light at approx.305 nm and approx.315 nm, which is particularly useful for ozone sensing using differential absorption lidar technique. For practical realization of space based UV transmitter technology, ceramic Nd:Y2O3 material is considered to possess great potential. A plasma melting and quenching method has been developed to produce Nd3+ doped powders for consolidation into Nd:Y2O3 ceramic laser materials. This far-from-equilibrium processing methodology allows higher levels of rare earth doping than can be achieved by equilibrium methods. The method comprises of two main steps: (a) plasma melting and quenching to generate dense, and homogeneous doped metastable powders, (b) pressure assisted consolidation of these powders by hot isostatic pressing to make dense nanocomposite ceramics. Using this process, several 1" x 1" ceramic cylinders have been produced. The infrared transmission of undoped Y2O3 ceramics was as high as approx.75% without anti-reflection coating. In the case of Nd:Y2O3 ceramics infrared transmission values of approx.50% were achieved. Furthermore, Nd:Y2O3 samples with dopant concentrations of up to approx.2 at. % were prepared without significant emission quenching.

  8. Treatment of urethral diseases with neodymium:YAG laser.

    PubMed

    Bloiso, G; Warner, R; Cohen, M

    1988-08-01

    Over a thirty-month period, a wide variety of common urethral problems were treated on an ambulatory basis, with the neodymium:yttrium-aluminum garnet (Nd:YAG) laser. When used discriminately, laser treatment appears to be an effective modality for the management of selected urethral strictures. Thus far, excellent results have been obtained in 30 of 31 cases of short strictures where laser urethrotomy was performed as the first stricture procedure (average follow-up 10 months). Furthermore, in a series of 36 cases of secondary bladder neck contractures, all of the evaluated patients responded well (average follow-up 7 months). Good results were obtained in only 11 of 48 complicated strictures (average follow-up 14 months). However, while most of these extensive strictures were not eradicated, laser therapy generally produced a documented clinical improvement, comparable to urethrotomy or dilatation, in 15 of these cases. A series of 24 condylomata involving the urethra were treated satisfactorily, with no recurrences (average follow-up 13 months). Laser treatment also has been used successfully for the management of several urethral caruncles, urethral polyps, two meatal hemangiomas, one urethral carcinoma, and a distal duplicated urethra. Recently, the Nd:YAG laser has been applied to the prostatic urethra with vaporization of obstructing median bar hyperplasia. Favorable results have been achieved in 5 of 6 cases treated with a newly developed technique that utilizes direct laser contact. Retrograde ejaculation has not been encountered in these patients (average follow-up 6 months). All of these procedures have been accomplished in the office, largely without urethral catheterization. Lidocaine jelly occasionally supplemented with intravenous sedation provided satisfactory anesthesia. PMID:3400132

  9. Effect of fulvic acid on neodymium uptake by goethite.

    PubMed

    Armstrong, Christopher R; Wood, Scott A

    2012-12-01

    Experimental studies of the interaction of aqueous neodymium (Nd), Suwannee River fulvic acid (FA), and solid phase goethite were conducted. Results from blank systems (individual Nd and FA), binary systems (Nd-goethite, FA-goethite, and Nd-FA), and ternary systems (Nd-FA-goethite) at 0.1 mol/kg and 25°C are reported. In the binary Nd-goethite system a classic sorption edge is observed, whereby virtually all Nd is removed from solution above the goethite point of zero charge (PZC). Similarly, the binary FA-goethite system exhibits strong FA sorption; However in this system near complete removal of FA from solution is observed below the goethite PZC. In the binary Nd-FA system, both aqueous Nd and FA feature a sharp decrease in concentration at ca. pH 9. Various experiments in the ternary system were conducted. For all concentrations, FA enhanced Nd sorption below the goethite PZC, attributed to the formation of a Type B ternary surface complex (mineral-ligand-metal ion). Notably, the 100 ppm FA ternary system showed anomalously high dissolved Nd in solution above the PZC (i.e., Nd sorption suppression) and a concomitant increase in goethite dissolution (∼9 ppm total Fe(3+) observed above circa pH 9.5). Our results suggest that Nd-FA complexation plays a key role in Nd uptake by goethite, and that this process is largely governed by pH: Whereas at pHs below the goethite PZC, Nd-FA complexation facilitates Nd sorption, above the PZC, and particularly at elevated FA concentrations, the formation of aqueous Nd-FA complexes suppresses Nd removal. Moreover, under these conditions, goethite dissolution may also play a role in mitigating Nd uptake by goethite. PMID:22958855

  10. miR-146b antagomir-treated human Tregs acquire increased GVHD inhibitory potency.

    PubMed

    Lu, Yunjie; Hippen, Keli L; Lemire, Amanda L; Gu, Jian; Wang, Weizhi; Ni, Xuhao; Ranganathan, Parvathi; Levine, Bruce L; Riley, James L; June, Carl H; Turka, Laurence A; Munn, David H; Garzon, Ramiro; Lu, Ling; Blazar, Bruce R

    2016-09-01

    CD4(+)CD25(+)FoxP3(+) thymic-derived regulatory T cells (tTregs) are indispensable for maintaining immune system equilibrium. Adoptive transfer of tTregs is an effective means of suppressing graft-versus-host disease (GVHD) in murine models and in early human clinical trials. Tumor necrosis factor receptor-associated factor 6 (TRAF6), an ubiquitin-conjugating enzyme that mediates nuclear factor κB (NF-κB) activation, plays an essential role in modulating regulatory T cell survival and function. MicroRNAs (miRNAs) are noncoding RNAs, which mediate RNA silencing and posttranscriptional gene repression. By performing comprehensive TaqMan Low Density Array miRNA assays, we identified 10 miRNAs differentially regulated in human tTreg compared with control T cells. One candidate, miR-146b, is preferentially and highly expressed in human naive tTregs compared with naive CD4 T cells. miRNA prediction software revealed that TRAF6 was the one of the top 10 scored mRNAs involved tTreg function with the highest probability as a potential miR-146b target. Antagomir-mediated knockdown of miRNA-146b, but not another miRNA-146 family member (miRNA-146a), enhanced TRAF6 expression. TRAF6, in turn, increases NF-κB activation, which is essential for tTreg function as well as Foxp3 protein and antiapoptotic gene expression, and downregulates proapoptotic gene expression. miR-146b knockdown increased the nuclear localization and expression of genes regulated by NF-κB, which was associated with enhanced tTreg survival, proliferation, and suppressive function measured in vitro and in vivo. TRAF6 inhibition had the opposite effects. We conclude that an miR-146b-TRAF6-NF-κB-FoxP3 signaling pathway restrains regulatory T cell survival, proliferation, and suppressor function. In vitro exposure of human tTregs to miR-146b antagomirs can be exploited to improve the clinical efficacy of human adoptive tTreg transfer in a GVHD setting. PMID:27485827

  11. Neodymium, strontium and chromium isotopic studies of the LEW86010 and Angra dos Reis meteorites and the chronology of the angrite parent body

    NASA Technical Reports Server (NTRS)

    Nyquist, L. E.; Bansal, B.; Wiesmann, H.; Shih, C.-Y.

    1994-01-01

    Neodymium, stontium, and chromium isotopic studies of the LEW86010 angrite established its absolute age and the formation interval between its crystallization and condensation of Allende CAIs from the solar nebula. Pyroxene and phosphate were found to contain approximately 8% of its Sm and Nd inventory. A conventional Sm-147-Nd-143 isochron yielded an age of 4.53 +/- 0.04 Ga (2 sigma and Epsilon(sub Nd sup 143)) = 0.45 +/- 1.1. An Sm-146-Nd-142 isochron gives initial Sm-146/Sm-144 = 0.0076 +/- 0.0009 and Epsilon (sub Nd sup 142) = -2.5 +/- 0.4. The Rb-Sr analyses give initial Sr-87/Sr-86 Iota(sub Sr sup 87) = 0.698972 +/- 8 and 0.698970 +/- 18 for LEW and ADOR, respectively, relative to Sr-87/Sr-86 = 0.71025 for NBS987. The difference, Delta Iota(sub Sr Sup 87), between Iota (sub sr sup 87) for the angrites and literature values for Allende CAIs, corresponds to approximately Ma of growth in a solar nebula with a CI chondrite value of Rb-87/Sr-86 = 0.91, or approximately 5 Ma in a nebula with solar photospheric Rb-87/Sr-86 = 1.51. Excess Cr-53 from extinct Mn-53(t(sub 1/2) = 3.7 Ma)in LEW86010 corresponds to initial Mn-53/Mn-55 = 4.4 +/- 1.0 x 10(exp -5) for the inclusions as previously reported by the Paris group (Birck and Allegre, 1988). The Sm-146/Sm-144 value found for LEW86010 corresponds to solar system initial (Sm-146/Sm-144) = 0.0080 +/- 0.0009 for crystallization 8 Ma after Allende, the difference between Pb-Pb ages of angrites and Allende, or 0.0086 +/- 0.0009 for crystallation 18 Ma after Allende, using the Mn-Cr formation interval. The isotopic data are discussed in the context of a model in which an undifferentiated 'chondritic' parent body formed from the solar nebula approximately Ma after Allende CAIs and subsequently underwent differentiation accompanied by loss of volatiles. Parent bodies with Rb/Sr similar to that of CI, CM, or CO chondrites could satisfy the Cr and Sr isotopic systematics. If the angrite parent body had Rb/Sr similar to that of

  12. Critical roles of CD146 in zebrafish vascular development.

    PubMed

    Chan, Barden; Sinha, Sonia; Cho, Dan; Ramchandran, Ramani; Sukhatme, Vikas P

    2005-01-01

    In this report, we use zebrafish as a model system to understand the importance of CD146 in vascular development. Endothelial-specific expression of CD146 was verified by whole-mount in situ hybridization. Suppression of CD146 protein expression by antisense morpholino oligonucleotides (MO) resulted in poorly developed intersomitic vessels (ISVs). In CD146 morphants, we observed a lack of blood flow through the ISV region, despite that fluorescence microangiography showed that the ISVs were present. This finding suggests that the lumens of the developing ISVs may be too narrow for proper circulation. Additionally, remodeling of the caudal vein plexus into functional vascular tubes appeared to be affected. Suppression of CD146 protein expression resulted in a circulation shunt that caused the circulation to by-pass part of the caudal artery/vein system. The same vascular defects were recapitulated by using a second morpholino oligonucleotide. This morphant expressed a truncated CD146 protein with amino acids V32 to T57 at the N terminus deleted in an in-frame manner. This region, therefore, is likely to contain elements critical for CD146 function. This study provides the first in vivo functional assessment of CD146 in embryonic development by showing that knockdown of CD146 protein expression severely hinders vascular development in zebrafish. PMID:15580611

  13. Samarium-neodymium systematics in kimberlites and in the minerals of garnet lherzolite inclusions

    USGS Publications Warehouse

    Basu, A.R.; Tatsumoto, M.

    1979-01-01

    The initial ratios of neodymium-143 to neodymium-144 in kimberlites ranging in age between 90 ?? 106 to 1300 ?? 106 years from South Africa, India, and the United States are different from the corresponding ratios in the minerals of peridotite inclusions in the kimberlites but are identical to the ratios in the basaltic achondrite Juvinas at the times of emplacement of the respective kimberlite pipes. This correlation between the kimberlites and Juvinas, which represents the bulk chondritic earth in rare-earth elements, strongly indicates that the kimberlite's source in the mantle is chondritic in rare-earth elements and relatively primeval in composition. Copyright ?? 1979 AAAS.

  14. Samarium-neodymium systematics in kimberlites and in the minerals of garnet lherzolite inclusions.

    PubMed

    Basu, A R; Tatsumoto, M

    1979-07-27

    The initial ratios of neodymium-143 to neodymium-144 in kimberlites ranging in age between 90 x 10(6) to 1300 x 10(6) years from South Africa, India, and the United States are different from the corresponding ratios in the minerals of peridotite inclusions in the kimberlites but are identical to the ratios in the basaltic achondrite Juvinas at the times of emplacement of the respective kimberlite pipes. This correlation between the kimberlites and Juvinas, which represents the bulk chondritic earth in rare-earth elements, strongly indicates that the kimberlite's source in the mantle is chondritic in rare-earth elements and relatively primeval in composition. PMID:17790851

  15. Equilibrium distribution of lanthanum, neodymium, and thorium between lithium chloride melt and liquid bismuth

    NASA Astrophysics Data System (ADS)

    Zagnit'ko, A. V.; Ignat'ev, V. V.

    2013-04-01

    The distribution of lanthanum, neodymium, and thorium between a lithium chloride melt and liquid bismuth with additions of lithium as a reducing agent are investigated at 650°C. Equilibrium values of their distribution constants are measured. It is shown that in contrast to neodymium and lanthanum, thorium cannot be extracted from bismuth into lithium chloride. This allows us to propose an efficient scheme for separating lanthanides and thorium in a system for the extraction of fuel salts in molten-salt nuclear reactors.

  16. Epitaxial neodymium-doped sapphire films, a new active medium for waveguide lasers.

    PubMed

    Kumaran, Raveen; Webster, Scott E; Penson, Shawn; Li, Wei; Tiedje, Thomas; Wei, Peng; Schiettekatte, Francois

    2009-11-01

    Epitaxial films of neodymium-doped sapphire have been grown by molecular beam epitaxy on R-, A-, and M-plane sapphire substrates. The emission spectrum features sharp lines consistent with single-site doping of the Nd(3+) ion into the host crystal. This material is believed to be a nonequilibrium phase, inaccessible by conventional high-temperature growth methods. Neodymium-doped sapphire has a promising lasing line at 1096 nm with an emission cross section of 11.9x10(-19) cm(2), similar to the 1064 nm line of Nd:YVO(4). PMID:19881593

  17. Presenilin 2 influences miR146 level and activity in microglia

    PubMed Central

    Jayadev, Suman; Case, Amanda; Alajajian, Betty; Eastman, Alison J.; Möller, Thomas; Garden, Gwenn A.

    2014-01-01

    Microglia, the resident innate immune cells of the CNS, are the primary defenders against microbes and critical to CNS remodeling. Dysregulation of microglial behavior can lead to unchecked pro-inflammatory activity and subsequent neurodegeneration. The molecular mechanisms leading to chronic inflammation and microglial dysfunction in neurodegenerative diseases are not well-understood. It is known that patients with Presenilin 2 (PS2) mutations develop autosomal dominant Alzheimer disease. We have shown that a lack of normal PS2 function is associated with exaggerated microglia pro-inflammatory responses in vitro. To identify pathways by which PS2 regulates microglia and determine how PS2 dysfunction may lead to altered inflammatory pathways, we pursued an unbiased array approach to assess differential expression of microRNAs between murine PS2 knockout (KO) and wild-type microglia. We identified miR146, a negative regulator of monocyte pro-inflammatory response, as constitutively down-regulated in PS2 KO microglia. Consistent with a state of miR146 suppression, we found that PS2 KO microglia express higher levels of the miR146 target protein interleukin-1 receptor-associated kinase-1, and have increased NFκB transcriptional activity. We hypothesize that PS2 impacts microglial responses through modulation of miR146a. PS2 dysfunction, through aging or mutation, may contribute to neurodegeneration by influencing the pro-inflammatory behavior of microglia. PMID:23952003

  18. Promotion of Hendra virus replication by microRNA 146a.

    PubMed

    Stewart, Cameron R; Marsh, Glenn A; Jenkins, Kristie A; Gantier, Michael P; Tizard, Mark L; Middleton, Deborah; Lowenthal, John W; Haining, Jessica; Izzard, Leonard; Gough, Tamara J; Deffrasnes, Celine; Stambas, John; Robinson, Rachel; Heine, Hans G; Pallister, Jackie A; Foord, Adam J; Bean, Andrew G; Wang, Lin-Fa

    2013-04-01

    Hendra virus is a highly pathogenic zoonotic paramyxovirus in the genus Henipavirus. Thirty-nine outbreaks of Hendra virus have been reported since its initial identification in Queensland, Australia, resulting in seven human infections and four fatalities. Little is known about cellular host factors impacting Hendra virus replication. In this work, we demonstrate that Hendra virus makes use of a microRNA (miRNA) designated miR-146a, an NF-κB-responsive miRNA upregulated by several innate immune ligands, to favor its replication. miR-146a is elevated in the blood of ferrets and horses infected with Hendra virus and is upregulated by Hendra virus in human cells in vitro. Blocking miR-146a reduces Hendra virus replication in vitro, suggesting a role for this miRNA in Hendra virus replication. In silico analysis of miR-146a targets identified ring finger protein (RNF)11, a member of the A20 ubiquitin editing complex that negatively regulates NF-κB activity, as a novel component of Hendra virus replication. RNA interference-mediated silencing of RNF11 promotes Hendra virus replication in vitro, suggesting that increased NF-κB activity aids Hendra virus replication. Furthermore, overexpression of the IκB superrepressor inhibits Hendra virus replication. These studies are the first to demonstrate a host miRNA response to Hendra virus infection and suggest an important role for host miRNAs in Hendra virus disease. PMID:23345523

  19. Optical model calculations of 14.6A GeV silicon fragmentation cross sections

    NASA Technical Reports Server (NTRS)

    Townsend, Lawrence W.; Khan, Ferdous; Tripathi, Ram K.

    1993-01-01

    An optical potential abrasion-ablation collision model is used to calculate hadronic dissociation cross sections for a 14.6 A GeV(exp 28) Si beam fragmenting in aluminum, tin, and lead targets. The frictional-spectator-interaction (FSI) contributions are computed with two different formalisms for the energy-dependent mean free path. These estimates are compared with experimental data and with estimates obtained from semi-empirical fragmentation models commonly used in galactic cosmic ray transport studies.

  20. Up-regulation of FGFBP1 signaling contributes to miR-146a-induced angiogenesis in human umbilical vein endothelial cells

    PubMed Central

    Zhu, Hua-yu; Bai, Wen-dong; Liu, Jia-qi; Zheng, Zhao; Guan, Hao; Zhou, Qin; Su, Lin-lin; Xie, Song-tao; Wang, Yun-chuan; Li, Jun; Li, Na; Zhang, Yi-jie; Wang, Hong-tao; Hu, Da-hai

    2016-01-01

    Recent microRNA expression profiling studies have documented an up-regulation of miR-146a in several angiogenesis models. However, the underlying molecular mechanism of miR-146a in the angiogenic activity of endothelial cells has not been clearly elucidated. The present study was aimed to evaluate whether miR-146a promotes angiogenesis in HUVECs by increasing FGFBP1 expression via directly targeting CREB3L1. miR-146a was over expressed in HUVECs via lentiviral-miR-146a. Expression profiling analysis found miR-146a over expression resulted in up-regulation of angiogenesis and cytokine activity associated genes including FGF2. Further a combination of bioinformatics and experimental analyses demonstrated the CREB3L1 as a bona fide functional target of miR-146a during angiogenesis. Moreover, CREB3L1 inhibited luciferase expression from FGFBP1 promoter containing only CRE elements. Furthermore, CREB3L1 inhibited FGFBP1 expression by binding to two CRE-like sites located at approximately −1780–1777 and −868–865 bp relative to the FGFBP1 transcription start site. Additionally, ectopic expression of CREB3L1 decreased miR-146a-induced FGF2 secretion. These findings indicate that the miR-146a-CREB3L1-FGFBP1 signaling axis plays an important role in the regulation of angiogenesis in HUVECs and provides a potential therapeutic target for anti-angiogenic therapeutics. PMID:27121396

  1. Global mining risk footprint of critical metals necessary for low-carbon technologies: the case of neodymium, cobalt, and platinum in Japan.

    PubMed

    Nansai, Keisuke; Nakajima, Kenichi; Kagawa, Shigemi; Kondo, Yasushi; Shigetomi, Yosuke; Suh, Sangwon

    2015-02-17

    Meeting the 2-degree global warming target requires wide adoption of low-carbon energy technologies. Many such technologies rely on the use of precious metals, however, increasing the dependence of national economies on these resources. Among such metals, those with supply security concerns are referred to as critical metals. Using the Policy Potential Index developed by the Fraser Institute, this study developed a new footprint indicator, the mining risk footprint (MRF), to quantify the mining risk directly and indirectly affecting a national economy through its consumption of critical metals. We formulated the MRF as a product of the material footprint (MF) of the consuming country and the mining risks of the countries where the materials are mined. A case study was conducted for the 2005 Japanese economy to determine the MF and MRF for three critical metals essential for emerging energy technologies: neodymium, cobalt and platinum. The results indicate that in 2005 the MFs generated by Japanese domestic final demand, that is, the consumption-based metal output of Japan, were 1.0 × 10(3) t for neodymium, 9.4 × 10(3) t for cobalt, and 2.1 × 10 t for platinum. Export demand contributes most to the MF, accounting for 3.0 × 10(3) t, 1.3 × 10(5) t, and 3.1 × 10 t, respectively. The MRFs of Japanese total final demand (domestic plus export) were calculated to be 1.7 × 10 points for neodymium, 4.5 × 10(-2) points for cobalt, and 5.6 points for platinum, implying that the Japanese economy is incurring a high mining risk through its use of neodymium. This country's MRFs are all dominated by export demand. The paper concludes by discussing the policy implications and future research directions for measuring the MFs and MRFs of critical metals. For countries poorly endowed with mineral resources, adopting low-carbon energy technologies may imply a shifting of risk from carbon resources to other natural resources, in particular critical metals, and a trade

  2. Magnetoresistance and magnetic ordering in praseodymium and neodymium hexaborides

    SciTech Connect

    Anisimov, M. A.; Bogach, A. V.; Glushkov, V. V.; Demishev, S. V.; Samarin, N. A.; Filipov, V. B.; Shitsevalova, N. Yu.; Kuznetsov, A. V.; Sluchanko, N. E.

    2009-11-15

    The magnetoresistance {Delta}{rho}/{rho} of single-crystal samples of praseodymium and neodymium hexaborides (PrB{sub 6} and NdB{sub 6}) has been measured at temperatures ranging from 2 to 20 K in a magnetic field of up to 80 kOe. The results obtained have revealed a crossover of the regime from a small negative magnetoresistance in the paramagnetic state to a large positive magnetoresistive effect in magnetically ordered phases of the PrB{sub 6} and NdB{sub 6} compounds. An analysis of the dependences {Delta}{rho}(H)/{rho} has made it possible to separate three contributions to the magnetoresistance for the compounds under investigation. In addition to the main negative contribution, which is quadratic in the magnetic field (-{Delta}{rho}/{rho} {proportional_to} H{sup 2}), a linear positive contribution ({Delta}{rho}/{rho} {proportional_to} H) and a nonlinear ferromagnetic contribution have been found. Upon transition to a magnetically ordered state, the linear positive component in the magnetoresistance of the PrB{sub 6} and NdB{sub 6} compounds becomes dominant, whereas the quadratic contribution to the negative magnetoresistance is completely suppressed in the commensurate magnetic phase of these compounds. The presence of several components in the magnetoresistance has been explained by assuming that, in the antiferromagnetic phases of PrB{sub 6} and NdB{sub 6}, ferromagnetic nanoregions (ferrons) are formed in the 5d band in the vicinity of the rareearth ions. The origin of the quadratic contribution to the negative magnetoresistance is interpreted in terms of the Yosida model, which takes into account scattering of conduction electrons by localized magnetic moments of rare-earth ions. Within the approach used, the local magnetic susceptibility {chi}{sub loc} has been estimated. It has been demonstrated that, in the temperature range T{sub N} < T < 20 K, the behavior of the local magnetic susceptibility {chi}{sub loc} for the compounds under investigation can

  3. 7 CFR 4287.146-4287.155 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 15 2011-01-01 2011-01-01 false 4287.146-4287.155 Section 4287.146-4287.155 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE SERVICING Servicing Business and Industry...

  4. 7 CFR 4287.146-4287.155 - [Reserved

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 15 2013-01-01 2013-01-01 false 4287.146-4287.155 Section 4287.146-4287.155 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE SERVICING Servicing Business and Industry...

  5. 7 CFR 4287.146-4287.155 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 15 2012-01-01 2012-01-01 false 4287.146-4287.155 Section 4287.146-4287.155 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE SERVICING Servicing Business and Industry...

  6. 7 CFR 4287.146-4287.155 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 15 2014-01-01 2014-01-01 false 4287.146-4287.155 Section 4287.146-4287.155 Agriculture Regulations of the Department of Agriculture (Continued) RURAL BUSINESS-COOPERATIVE SERVICE AND RURAL UTILITIES SERVICE, DEPARTMENT OF AGRICULTURE SERVICING Servicing Business and Industry...

  7. 22 CFR 146.520 - Job classification and structure.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 22 Foreign Relations 1 2013-04-01 2013-04-01 false Job classification and structure. 146.520... Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  8. 22 CFR 146.520 - Job classification and structure.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 22 Foreign Relations 1 2012-04-01 2012-04-01 false Job classification and structure. 146.520... Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  9. 22 CFR 146.520 - Job classification and structure.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 22 Foreign Relations 1 2010-04-01 2010-04-01 false Job classification and structure. 146.520... Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  10. 22 CFR 146.520 - Job classification and structure.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 22 Foreign Relations 1 2014-04-01 2014-04-01 false Job classification and structure. 146.520... Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  11. 22 CFR 146.520 - Job classification and structure.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 22 Foreign Relations 1 2011-04-01 2011-04-01 false Job classification and structure. 146.520... Basis of Sex in Employment in Education Programs or Activities Prohibited § 146.520 Job classification and structure. A recipient shall not: (a) Classify a job as being for males or for females;...

  12. 13 CFR Appendix A to Part 146 - Certification Regarding Lobbying

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Certification Regarding Lobbying A... LOBBYING Pt. 146, App. A Appendix A to Part 146—Certification Regarding Lobbying Certification for... Standard Form-LLL, “Disclosure Form to Report Lobbying,” in accordance with its instructions. (3)...

  13. 12 CFR 390.146 - Equal Housing Lender Poster.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 5 2014-01-01 2014-01-01 false Equal Housing Lender Poster. 390.146 Section....146 Equal Housing Lender Poster. (a) Each State savings association shall post and maintain one or more Equal Housing Lender Posters, the text of which is prescribed in paragraph (b) of this section,...

  14. 12 CFR 390.146 - Equal Housing Lender Poster.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 5 2012-01-01 2012-01-01 false Equal Housing Lender Poster. 390.146 Section....146 Equal Housing Lender Poster. (a) Each State savings association shall post and maintain one or more Equal Housing Lender Posters, the text of which is prescribed in paragraph (b) of this section,...

  15. 12 CFR 390.146 - Equal Housing Lender Poster.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 5 2013-01-01 2013-01-01 false Equal Housing Lender Poster. 390.146 Section....146 Equal Housing Lender Poster. (a) Each State savings association shall post and maintain one or more Equal Housing Lender Posters, the text of which is prescribed in paragraph (b) of this section,...

  16. 33 CFR 110.146 - Long Island Sound.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... “Regulated Navigation Area: Long Island Sound Marine Inspection and Captain of the Port Zone,” 33 CFR 165.153... 33 Navigation and Navigable Waters 1 2012-07-01 2012-07-01 false Long Island Sound. 110.146... ANCHORAGE REGULATIONS Anchorage Grounds § 110.146 Long Island Sound. (a) Anchorage grounds. (1)...

  17. 33 CFR 110.146 - Long Island Sound.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... “Regulated Navigation Area: Long Island Sound Marine Inspection and Captain of the Port Zone,” 33 CFR 165.153... 33 Navigation and Navigable Waters 1 2014-07-01 2014-07-01 false Long Island Sound. 110.146... ANCHORAGE REGULATIONS Anchorage Grounds § 110.146 Long Island Sound. (a) Anchorage grounds. (1)...

  18. 33 CFR 110.146 - Long Island Sound.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... “Regulated Navigation Area: Long Island Sound Marine Inspection and Captain of the Port Zone,” 33 CFR 165.153... 33 Navigation and Navigable Waters 1 2011-07-01 2011-07-01 false Long Island Sound. 110.146... ANCHORAGE REGULATIONS Anchorage Grounds § 110.146 Long Island Sound. (a) Anchorage grounds. (1)...

  19. 33 CFR 110.146 - Long Island Sound.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... “Regulated Navigation Area: Long Island Sound Marine Inspection and Captain of the Port Zone,” 33 CFR 165.153... 33 Navigation and Navigable Waters 1 2013-07-01 2013-07-01 false Long Island Sound. 110.146... ANCHORAGE REGULATIONS Anchorage Grounds § 110.146 Long Island Sound. (a) Anchorage grounds. (1)...

  20. 12 CFR 252.146 - Methodologies and practices.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 12 Banks and Banking 4 2014-01-01 2014-01-01 false Methodologies and practices. 252.146 Section 252.146 Banks and Banking FEDERAL RESERVE SYSTEM (CONTINUED) BOARD OF GOVERNORS OF THE FEDERAL RESERVE SYSTEM (CONTINUED) ENHANCED PRUDENTIAL STANDARDS (REGULATION YY) Company-Run Stress Test Requirements...

  1. 19 CFR 146.61 - Constructive transfer to Customs territory.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Constructive transfer to Customs territory. 146.61 Section 146.61 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... Constructive transfer to Customs territory. The port director shall accept receipt of any entry in proper...

  2. 19 CFR 146.61 - Constructive transfer to Customs territory.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Constructive transfer to Customs territory. 146.61 Section 146.61 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... Constructive transfer to Customs territory. The port director shall accept receipt of any entry in proper...

  3. 19 CFR 146.61 - Constructive transfer to Customs territory.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Constructive transfer to Customs territory. 146.61 Section 146.61 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... Constructive transfer to Customs territory. The port director shall accept receipt of any entry in proper...

  4. 7 CFR 58.146 - Cleaning and sanitizing treatment.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Cleaning and sanitizing treatment. 58.146 Section 58.146 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) REGULATIONS AND STANDARDS UNDER THE AGRICULTURAL MARKETING...

  5. 21 CFR 146.126 - Frozen concentrate for colored lemonade.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 2 2014-04-01 2014-04-01 false Frozen concentrate for colored lemonade. 146.126... Fruit Juices and Beverages § 146.126 Frozen concentrate for colored lemonade. (a) Frozen concentrate for colored lemonade conforms to the definition and standard of identity prescribed for frozen concentrate...

  6. 21 CFR 146.126 - Frozen concentrate for colored lemonade.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 2 2013-04-01 2013-04-01 false Frozen concentrate for colored lemonade. 146.126... Fruit Juices and Beverages § 146.126 Frozen concentrate for colored lemonade. (a) Frozen concentrate for colored lemonade conforms to the definition and standard of identity prescribed for frozen concentrate...

  7. 21 CFR 146.126 - Frozen concentrate for colored lemonade.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 2 2012-04-01 2012-04-01 false Frozen concentrate for colored lemonade. 146.126... Fruit Juices and Beverages § 146.126 Frozen concentrate for colored lemonade. (a) Frozen concentrate for colored lemonade conforms to the definition and standard of identity prescribed for frozen concentrate...

  8. 21 CFR 146.126 - Frozen concentrate for colored lemonade.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 2 2010-04-01 2010-04-01 false Frozen concentrate for colored lemonade. 146.126... Fruit Juices and Beverages § 146.126 Frozen concentrate for colored lemonade. (a) Frozen concentrate for colored lemonade conforms to the definition and standard of identity prescribed for frozen concentrate...

  9. 21 CFR 146.126 - Frozen concentrate for colored lemonade.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 2 2011-04-01 2011-04-01 false Frozen concentrate for colored lemonade. 146.126... Fruit Juices and Beverages § 146.126 Frozen concentrate for colored lemonade. (a) Frozen concentrate for colored lemonade conforms to the definition and standard of identity prescribed for frozen concentrate...

  10. 50 CFR 665.146-665.159 - [Reserved

    Code of Federal Regulations, 2012 CFR

    2012-10-01

    ... 50 Wildlife and Fisheries 13 2012-10-01 2012-10-01 false 665.146-665.159 Section 665.146-665.159 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES IN THE WESTERN PACIFIC American Samoa Fisheries §§...

  11. 50 CFR 665.146-665.159 - [Reserved

    Code of Federal Regulations, 2014 CFR

    2014-10-01

    ... 50 Wildlife and Fisheries 13 2014-10-01 2014-10-01 false 665.146-665.159 Section 665.146-665.159 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES IN THE WESTERN PACIFIC American Samoa Fisheries §§...

  12. 50 CFR 665.146-665.159 - [Reserved

    Code of Federal Regulations, 2011 CFR

    2011-10-01

    ... 50 Wildlife and Fisheries 11 2011-10-01 2011-10-01 false 665.146-665.159 Section 665.146-665.159 Wildlife and Fisheries FISHERY CONSERVATION AND MANAGEMENT, NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, DEPARTMENT OF COMMERCE (CONTINUED) FISHERIES IN THE WESTERN PACIFIC American Samoa Fisheries §§...

  13. 13 CFR 146.600 - Semi-annual compilation.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Semi-annual compilation. 146.600 Section 146.600 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION NEW RESTRICTIONS ON LOBBYING.... (c) Information that involves intelligence matters shall be reported only to the Select Committee...

  14. 19 CFR 146.61 - Constructive transfer to Customs territory.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 19 Customs Duties 2 2011-04-01 2011-04-01 false Constructive transfer to Customs territory. 146.61 Section 146.61 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... Constructive transfer to Customs territory. The port director shall accept receipt of any entry in proper...

  15. 19 CFR 146.61 - Constructive transfer to Customs territory.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Constructive transfer to Customs territory. 146.61 Section 146.61 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... Constructive transfer to Customs territory. The port director shall accept receipt of any entry in proper...

  16. 19 CFR 146.93 - Inventory control and recordkeeping system.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 19 Customs Duties 2 2014-04-01 2014-04-01 false Inventory control and recordkeeping system. 146.93 Section 146.93 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... producibility method of attribution requires that records be kept to attribute final products to...

  17. 19 CFR 146.93 - Inventory control and recordkeeping system.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Inventory control and recordkeeping system. 146.93 Section 146.93 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... producibility method of attribution requires that records be kept to attribute final products to...

  18. 19 CFR 146.96 - Approval of other recordkeeping systems.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 19 Customs Duties 2 2012-04-01 2012-04-01 false Approval of other recordkeeping systems. 146.96 Section 146.96 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade...

  19. 19 CFR 146.93 - Inventory control and recordkeeping system.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 19 Customs Duties 2 2013-04-01 2013-04-01 false Inventory control and recordkeeping system. 146.93 Section 146.93 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY... producibility method of attribution requires that records be kept to attribute final products to...

  20. 19 CFR 146.96 - Approval of other recordkeeping systems.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 19 Customs Duties 2 2010-04-01 2010-04-01 false Approval of other recordkeeping systems. 146.96 Section 146.96 Customs Duties U.S. CUSTOMS AND BORDER PROTECTION, DEPARTMENT OF HOMELAND SECURITY; DEPARTMENT OF THE TREASURY (CONTINUED) FOREIGN TRADE ZONES Petroleum Refineries in Foreign-Trade...