Science.gov

Sample records for nick kettridge andrew

  1. Andrew W. S. In

    ERIC Educational Resources Information Center

    Nishimoto, Warren

    2007-01-01

    This article presents an interview with Andrew W. S. In, professor and dean in the University of Hawai'i's College of Education from 1951 to 1984. Born and raised in Honolulu, In attended Royal Elementary, Central Junior High, and McKinley High schools, graduating from McKinley in 1938. He then attended the University of Hawai'i Teachers College…

  2. Christina's World: Andrew Wyeth.

    ERIC Educational Resources Information Center

    Niceley, H. T.

    1990-01-01

    Describes and illustrates "Christina's World" by Andrew Wyeth. Provides background information on Wyeth's life and compares "Christina's World" to Wyeth's "Siri." Suggests activities to help all levels of art students understand use of color, mood, and composition. Introduces related activities designed specifically for elementary school students.…

  3. Andrew: CMU's New Computing Environment.

    ERIC Educational Resources Information Center

    Zabowski, Susan

    1986-01-01

    Reviews the progress and problems associated with the development of Carnegie Mellon University's new computing and communications system, "Andrew." Describes the accomplishments and capacities of the system and provides examples of the programs developed for "Andrew." (ML)

  4. Stories Along the Way: Andrew

    ERIC Educational Resources Information Center

    Kittle, Penny

    2009-01-01

    Andrew is among the forgotten, the written-off, the hopeless...at least to many of the adults in his life--police, teachers, even family. But when you see that glimmer, that potential, you can't turn your back. Andrew stands as a person, but also a metaphor for believing that each student has a chance to succeed.

  5. Faces of Marshall: Erika Andrews

    NASA Video Gallery

    Several Marshall employees were interviewed as part of Marshall's 50th Anniversary activities. Human Resources Specialist Erika Andrews tells how she came to work at NASA as a specialist in organiz...

  6. Sequence-specific DNA nicking endonucleases.

    PubMed

    Xu, Shuang-yong

    2015-08-01

    A group of small HNH nicking endonucleases (NEases) was discovered recently from phage or prophage genomes that nick double-stranded DNA sites ranging from 3 to 5 bp in the presence of Mg2+ or Mn2+. The cosN site of phage HK97 contains a gp74 nicking site AC↑CGC, which is similar to AC↑CGR (R=A/G) of N.ϕGamma encoded by Bacillus phage Gamma. A minimal nicking domain of 76 amino acid residues from N.ϕGamma could be fused to other DNA binding partners to generate chimeric NEases with new specificities. The biological roles of a few small HNH endonucleases (HNHE, gp74 of HK97, gp37 of ϕSLT, ϕ12 HNHE) have been demonstrated in phage and pathogenicity island DNA packaging. Another group of NEases with 3- to 7-bp specificities are either natural components of restriction systems or engineered from type IIS restriction endonucleases. A phage group I intron-encoded HNH homing endonucleases, I-PfoP3I was found to nick DNA sites of 14-16 bp. I-TslI encoded by T7-like ΦI appeared to nick DNA sites with a 9-bp core sequence. DNA nicking and labeling have been applied to optical mapping to aid genome sequence assembly and detection of large insertion/deletion mutations in genomic DNA of cancer cells. Nicking enzyme-mediated amplification reaction has been applied to rapid diagnostic testing of influenza A and B in clinical setting and for construction of DNA-based Boolean logic gates. The clustered regularly interspaced short palindromic repeats-ribonucleoprotein complex consisting of engineered Cas9 nickases in conjunction with tracerRNA:crRNA or a single-guide RNA have been successfully used in genome modifications. PMID:26352356

  7. Libraries and Andrew Carnegie's Challenge.

    ERIC Educational Resources Information Center

    Gregorian, Vartan

    This essay by the president of the Carnegie Corporation of New York (reprinted from the 1998 annual report) opens by noting that the year 1999 marks the 100th anniversary of Andrew Carnegie's support for the planning and development of 65 branch libraries of the New York Public Library System, a gift that came to more than $5.2 million. Discussion…

  8. Benevolent Builder: Appraising Andrew Carnegie.

    ERIC Educational Resources Information Center

    Deitch, Joseph

    1984-01-01

    Discussion of Andrew Carnegie era in American public library history focuses on library buildings financed and built in Philadelphia, Pennsylvania. The building design which Carnegie developed, surviving Carnegie libraries in Philadelphia, procedures for obtaining a library, and comparison of architectural attractiveness of Carnegie libraries with…

  9. The McAndrews Leadership Lecture: Origins

    PubMed Central

    Hamm, Anthony W.; Burkhart, Lori A.

    2015-01-01

    Objective This article describes the origins and rationale for the McAndrews Leadership Lecture and explains why the American Chiropractic Association honors George and Jerome McAndrews. Discussion George and Jerome McAndrews’ backgrounds demonstrate their leadership contributions to the chiropractic profession. Jerome McAndrews, a chiropractor, held substantial leadership roles in the chiropractic profession. George McAndrews, a lawyer, administered a permanent injunction forbidding the American Medical Association’s restraint of trade toward the chiropractic profession. Conclusion The American Chiropractic Association has established the McAndrews Leadership Lecture to honor their contributions to the chiropractic profession. PMID:26770176

  10. Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks

    PubMed Central

    Vriend, Lianne E.M.; Prakash, Rohit; Chen, Chun-Chin; Vanoli, Fabio; Cavallo, Francesca; Zhang, Yu; Jasin, Maria; Krawczyk, Przemek M.

    2016-01-01

    DNA double-strand breaks (DSBs) are known to be powerful inducers of homologous recombination (HR), but single-strand breaks (nicks) have also been shown to trigger HR. Both DSB- and nick-induced HR (nickHR) are exploited in advanced genome-engineering approaches based on the bacterial RNA-guided nuclease Cas9. However, the mechanisms of nickHR are largely unexplored. Here, we applied Cas9 nickases to study nickHR in mammalian cells. We find that nickHR is unaffected by inhibition of major damage signaling kinases and that it is not suppressed by nonhomologous end-joining (NHEJ) components, arguing that nick processing does not require a DSB intermediate to trigger HR. Relative to a single nick, nicking both strands enhances HR, consistent with a DSB intermediate, even when nicks are induced up to ∼1kb apart. Accordingly, HR and NHEJ compete for repair of these paired nicks, but, surprisingly, only when 5' overhangs or blunt ends can be generated. Our study advances the understanding of molecular mechanisms driving nick and paired-nick repair in mammalian cells and clarify phenomena associated with Cas9-mediated genome editing. PMID:27001513

  11. Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA

    PubMed Central

    Gutjahr, Alice; Xu, Shuang-yong

    2014-01-01

    N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated fusion enzymes that preferentially nick 5mCG-modified DNA. The CG dinucleotide can be modified by C5 methyltransferases (MTases) such as M.SssI, M.HhaI or M.HpaII to create composite sites AC↑YGG N(8–15) 5mCG. We also constructed a fusion enzyme 2xMBD2-ND(N.BceSVIII) targeting more frequent composite sites AS↑YS N(5–12) 5mCG in Mn2+ buffer. 5mCG-dependent nicking requires special digestion conditions in high salt (0.3 M KCl) or in Ni2+ buffer. The fusion enzyme can be used to nick and label 5mCG-modified plasmid and genomic DNAs with fluorescently labeled Cy3-dUTP and potentially be useful for diagnostic applications, DNA sequencing and optical mapping of epigenetic markers. The importance of the predicted catalytic residues D89, H90, N106 and H115 in N.ϕGamma was confirmed by mutagenesis. We found that the wild-type enzyme N.ϕGamma prefers to nick 5mCG-modified DNA in Ni2+ buffer even though the nicking activity is sub-optimal compared to the activity in Mg2+ buffer. PMID:24609382

  12. The Impeachment of Andrew Johnson. Web Lesson.

    ERIC Educational Resources Information Center

    Constitutional Rights Foundation, Los Angeles, CA.

    This lesson presents the historical background of Abraham Lincoln's selection of Andrew Johnson as his running mate in the election of 1864. The lesson considers the climate in the U.S. Congress after President Lincoln's assassination. The details of the impeachment and trial of President Andrew Johnson are given. The lesson presents three…

  13. GEN1 promotes Holliday junction resolution by a coordinated nick and counter-nick mechanism

    PubMed Central

    Chan, Ying Wai; West, Stephen

    2015-01-01

    Holliday junctions (HJs) that physically link sister chromatids or homologous chromosomes are formed as intermediates during DNA repair by homologous recombination. Persistent recombination intermediates are acted upon by structure-selective endonucleases that are required for proper chromosome segregation at mitosis. Here, we have purified full-length human GEN1 protein and show that it promotes Holliday junction resolution by a mechanism that is analogous to that exhibited by the prototypic HJ resolvase E. coli RuvC. We find that GEN1 cleaves HJs by a nick and counter-nick mechanism involving dual co-ordinated incisions that lead to the formation of ligatable nicked duplex products. As observed with RuvC, cleavage of the first strand is rate limiting, while second strand cleavage is rapid. In contrast to RuvC, however, GEN1 is largely monomeric in solution, but dimerizes on the HJ. Using HJs containing non-cleavable phosphorothioate-containing linkages in one strand, we show that the two incisions can be uncoupled and that the first nick occurs upon GEN1 dimerization at the junction. These results indicate that the mechanism of HJ resolution is largely conserved from bacteria to man, despite a lack of sequence homology between the resolvases. PMID:26578604

  14. Obituary: Andrew Stephen Wilson, 1947-2008

    NASA Astrophysics Data System (ADS)

    Veilleux, Sylvain

    2009-01-01

    On 24 May 2008, Andrew Stephen Wilson passed away at the age of 61, in his home in Silver Spring, Maryland, from complications resulting from a painful spinal illness. Andrew was arguably one of the first truly multi-wavelength astronomers of his generation. His scientific work on active galactic nuclei [AGN] spanned the entire electromagnetic spectrum from the radio to the X-rays. Andrew was born in Doncaster, Yorkshire, England, on 26 March 1947. He was the younger of two brothers whose births were separated by the Second World War. His father, Norman, came from a relatively affluent family who were coal merchants. His mother, Mary, came from a less comfortable background, one of seven children, daughter of a skilled cabinet maker/French polisher, who went through a very hard time during the depression. As a teacher, she placed enormous value on hard work and education as a way of gaining advancement in life. When Andrew was four, the family moved to Skipton, a nice market town in the Yorkshire dales. Andrew went to a small village school until age eleven when he entered Ermysted's Grammar School. He was an enthusiastic soccer and cricket player. He never lost his enthusiasm for soccer and supported the local soccer team, Leeds United, for all his life. Andrew also followed the Yorkshire county cricket team. Andrew's interest in astronomy stemmed from the fact that at Ermysted's Grammar School someone donated a four-inch refracting telescope, so he and his friends used to go back in the evenings to investigate the rings of Saturn, the moons of Jupiter, and various nebulae. While an undergraduate at Cambridge, Andrew joined the astronomy club and ground an 8-inch mirror by hand as a part of a telescope that he set up in the backyard of his parents' house. Andrew spent hours observing with this telescope, and it was the wonder of the family. At Cambridge, Andrew obtained his bachelor's degree with first-class honors in 1969. During a short visit in London with his

  15. Congruences for the Andrews spt function.

    PubMed

    Ono, Ken

    2011-01-11

    Ramanujan-type congruences for the Andrews spt(n) partition function have been found for prime moduli 5 ≤ ℓ ≤ 37 in the work of Andrews [Andrews GE, (2008) J Reine Angew Math 624:133-142] and Garvan [Garvan F, (2010) Int J Number Theory 6:1-29]. We exhibit unexpectedly simple congruences for all ℓ≥5. Confirming a conjecture of Garvan, we show that if ℓ≥5 is prime and (-δ/ℓ) = 1, then spt[(ℓ2(ℓn+δ)+1)/24] ≡ 0 (mod ℓ). This congruence gives (ℓ - 1)/2 arithmetic progressions modulo ℓ(3) which support a mod ℓ congruence. This result follows from the surprising fact that the reduction of a certain mock theta function modulo ℓ, for every ℓ≥5, is an eigenform of the Hecke operator T(ℓ(2)). PMID:21177432

  16. Obituary: Andrew Stephen Wilson, 1947-2008

    NASA Astrophysics Data System (ADS)

    Veilleux, Sylvain

    2009-01-01

    On 24 May 2008, Andrew Stephen Wilson passed away at the age of 61, in his home in Silver Spring, Maryland, from complications resulting from a painful spinal illness. Andrew was arguably one of the first truly multi-wavelength astronomers of his generation. His scientific work on active galactic nuclei [AGN] spanned the entire electromagnetic spectrum from the radio to the X-rays. Andrew was born in Doncaster, Yorkshire, England, on 26 March 1947. He was the younger of two brothers whose births were separated by the Second World War. His father, Norman, came from a relatively affluent family who were coal merchants. His mother, Mary, came from a less comfortable background, one of seven children, daughter of a skilled cabinet maker/French polisher, who went through a very hard time during the depression. As a teacher, she placed enormous value on hard work and education as a way of gaining advancement in life. When Andrew was four, the family moved to Skipton, a nice market town in the Yorkshire dales. Andrew went to a small village school until age eleven when he entered Ermysted's Grammar School. He was an enthusiastic soccer and cricket player. He never lost his enthusiasm for soccer and supported the local soccer team, Leeds United, for all his life. Andrew also followed the Yorkshire county cricket team. Andrew's interest in astronomy stemmed from the fact that at Ermysted's Grammar School someone donated a four-inch refracting telescope, so he and his friends used to go back in the evenings to investigate the rings of Saturn, the moons of Jupiter, and various nebulae. While an undergraduate at Cambridge, Andrew joined the astronomy club and ground an 8-inch mirror by hand as a part of a telescope that he set up in the backyard of his parents' house. Andrew spent hours observing with this telescope, and it was the wonder of the family. At Cambridge, Andrew obtained his bachelor's degree with first-class honors in 1969. During a short visit in London with his

  17. Obituary: Andrew Lange (1957-2010)

    NASA Astrophysics Data System (ADS)

    Kamionkowski, Marc

    2011-12-01

    The worlds of physics and astrophysics were stunned to learn on 22 January 2010 that Andrew Lange, the Marvin L. Goldberger Professor of Physics at Caltech, had taken his own life the night before. He had succumbed to the severe depression that he had suffered from for many years, unbeknownst to even his closest colleagues. Lange will perhaps be best remembered as the co-leader of Boomerang, the balloon-borne experiment that provided the first high-angular-resolution map of the cosmic microwave background (CMB). And while this was certainly his most notable achievement, Andrew amassed a record of accomplishment as an instrumentalist, leader, mentor, and communicator that extended much further. Andrew was born in Urbana, Illinois on July 23, 1957, the son of an architect and a librarian, and raised primarily in Connecticut. His family and early friends remember him as a serious and extremely intelligent child and young man. Andrew Lange's lifelong interest in the CMB was nurtured as an undergraduate at Princeton University by David Wilkinson, and he recalled fondly a summer spent working with John Mather at Goddard Space Flight Center. Andrew Lange went to graduate school in physics at Berkeley where he worked in Paul Richards' group. Although his thesis project, the Berkeley-Nagoya rocket experiment, showed an anomalous sub-millimeter excess in the CMB spectrum that was shortly thereafter shown by a later flight of the same rocket and COBE-FIRAS to be incorrect, Lange's talents were recognized by the physics department at Berkeley who appointed him shortly after his PhD (1987) to their faculty. While on the Berkeley faculty, Andrew obtained early detections of the Sunyaev-Zeldovich effect, upper limits to small-angle CMB fluctuations, and important infrared constraints to the interstellar medium. He also led a pioneering instrument operating 300 mK detectors for a small infrared satellite experiment. This early work showed high ambition and daring, and it pioneered

  18. A History of the Andrew File System

    SciTech Connect

    2011-02-22

    Derrick Brashear and Jeffrey Altman will present a technical history of the evolution of Andrew File System starting with the early days of the Andrew Project at Carnegie Mellon through the commercialization by Transarc Corporation and IBM and a decade of OpenAFS. The talk will be technical with a focus on the various decisions and implementation trade-offs that were made over the course of AFS versions 1 through 4, the development of the Distributed Computing Environment Distributed File System (DCE DFS), and the course of the OpenAFS development community. The speakers will also discuss the various AFS branches developed at the University of Michigan, Massachusetts Institute of Technology and Carnegie Mellon University.

  19. Andrew shortens lifetime of Louisiana Barrier Islands

    NASA Astrophysics Data System (ADS)

    Bush, Susan

    Because the Isles Dernieres, a series of four barrier islands off the coast of Louisiana, have one of the most rapidly eroding shorelines in the world, geologists at the U.S. Geological Survey and the Louisiana Geological Survey have been monitoring erosion activity over the last several years, said Jeff Williams of the USGS in Reston, Va. Hurricane Andrew, which struck the state on August 26, caused severe erosional damage to these islands that has shortened their lifetimes.Before Andrew struck, geologists projected that Raccoon Island would disappear below sea level by the year 2001 and that Whiskey Island would disappear by 2016. Now, due to the severe erosion from Hurricane Andrew, the scientists claim that the islands may disappear before the turn of the century, and the other islands in the Dernieres chain are expected to follow suit within 2 decades. Raccoon, Whiskey, Trinity, and East islands make up the Isles Dernieres, which existed as one island, known as the Isle Derniere, before an 1856 hurricane and subsequent erosion.

  20. Oxygen and Metastasis: A Conversation with Dr. Nick Restifo

    Cancer.gov

    Dr. Nick Restifo, a senior investigator in NCI’s Center for Cancer Research, discusses his recently published study finding that Oxygen, a molecule necessary for life, paradoxically aids cancer metastasis to the lung by impairing cancer-killing immune cells.

  1. Obituary: Andrew Lange (1957-2010)

    NASA Astrophysics Data System (ADS)

    Kamionkowski, Marc

    2011-12-01

    The worlds of physics and astrophysics were stunned to learn on 22 January 2010 that Andrew Lange, the Marvin L. Goldberger Professor of Physics at Caltech, had taken his own life the night before. He had succumbed to the severe depression that he had suffered from for many years, unbeknownst to even his closest colleagues. Lange will perhaps be best remembered as the co-leader of Boomerang, the balloon-borne experiment that provided the first high-angular-resolution map of the cosmic microwave background (CMB). And while this was certainly his most notable achievement, Andrew amassed a record of accomplishment as an instrumentalist, leader, mentor, and communicator that extended much further. Andrew was born in Urbana, Illinois on July 23, 1957, the son of an architect and a librarian, and raised primarily in Connecticut. His family and early friends remember him as a serious and extremely intelligent child and young man. Andrew Lange's lifelong interest in the CMB was nurtured as an undergraduate at Princeton University by David Wilkinson, and he recalled fondly a summer spent working with John Mather at Goddard Space Flight Center. Andrew Lange went to graduate school in physics at Berkeley where he worked in Paul Richards' group. Although his thesis project, the Berkeley-Nagoya rocket experiment, showed an anomalous sub-millimeter excess in the CMB spectrum that was shortly thereafter shown by a later flight of the same rocket and COBE-FIRAS to be incorrect, Lange's talents were recognized by the physics department at Berkeley who appointed him shortly after his PhD (1987) to their faculty. While on the Berkeley faculty, Andrew obtained early detections of the Sunyaev-Zeldovich effect, upper limits to small-angle CMB fluctuations, and important infrared constraints to the interstellar medium. He also led a pioneering instrument operating 300 mK detectors for a small infrared satellite experiment. This early work showed high ambition and daring, and it pioneered

  2. 76 FR 48168 - Andrew K. Choi: Debarment Order

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-08

    ... HUMAN SERVICES Food and Drug Administration Andrew K. Choi: Debarment Order AGENCY: Food and Drug... the Federal Food, Drug, and Cosmetic Act (the FD&C Act) debarring Andrew K. Choi, M.D. for 4 years... K. Choi has been convicted of a misdemeanor under Federal law for conduct relating to the...

  3. Introduction of Dr. Andrew V Schally

    PubMed Central

    Mendoza-Valdés, Arturo

    2015-01-01

    I first met Dr. Andrew V Schally (PhD, MDhc (Multi), DSc, Distinguished Medical Research Scientist, U.S. Department of Veterans Affairs Professor of Pathology and Department of Medicine,
Miller School of Medicine, University of Miami, Miami, Florida, USA) many years ago, probably around the beginning of the 1990's in one of his visits to Mexico City (Figure 1). He has many friends in my country since some of the investigations that led to the development of the LHRH agonists were made in a couple of Mexican hospitals in collaboration with some outstanding Mexican physicians that I will mention later. In that time, I was the head of the Department of Urology of the Mexican National Cancer Institute and our Director, Dr. Jaime de la Garza, invited him for a meeting. I was surprised by his humbleness, intelligence and easy going personality, in spite of being a Nobel Prize scientist. PMID:26112485

  4. Structural Basis for Nick Recognition by a Minimal Pluripotent DNA Ligase

    SciTech Connect

    Nair,P.; Nandakumar, J.; Smith, P.; Odell, M.; Lima, C.; Shuman, S.

    2007-01-01

    Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-{angstrom} crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO{sub 4} nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a {beta}-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO{sub 4} termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.

  5. Fred Plaut in conversation with Andrew Samuels. Interview by Andrew Samuels.

    PubMed

    Plaut, Fred

    2010-02-01

    This is a reprint of an interview of Fred Plaut (who died in June 2009) conducted by Andrew Samuels in mid-1988 and first published in April 1989 in the Journal, 34, 2, pp. 159-83. The interview covers Plaut's early life, his career, and historical observations of the development of the Society of Analytical Psychology from its beginnings, and of the wider community of Jungian analysis. Plaut reflects uninhibitedly on such topics as the role of leadership in analytical psychology, discussing the parts played by Michael Fordham in London and Hannes Dieckmann in Berlin. Plaut explains his thinking concerning individuation. PMID:20433496

  6. 3. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 2, 1936 PANELS AND VAULTED CEILING IN DRAWING ROOM, SECOND FLOOR - Emerson & Holmes Building, 556 Mulberry Street, Macon, Bibb County, GA

  7. 5. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    5. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 2, 1936 DETAIL OF DOUBLE OPENING WITH IRON GRATING IN THIRD STORY REAR ROOM - Emerson & Holmes Building, 556 Mulberry Street, Macon, Bibb County, GA

  8. 4. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    4. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 19, 1936 STAIRWAY AND REAR DOOR THROUGH OPENING Between NORTHEAST ROOM AND HALL - Ross Crane House, Pulaski & Washington Streets, Athens, Clarke County, GA

  9. 1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photographed by Harold Bush-Brown Nov. 14, 1936 GENERAL VIEW OF SLAVE CABINS - Bass Place (Slave Cabins), Columbus, Muscogee County, GA

  10. 2. Historic American Buildings Survey L. D. Andrew, Photographer, Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer, Enlarged Photographed by Harold Bush-Brown Nov. 14, 1936 VIEW OF EASTERN SLAVE CABIN - Bass Place (Slave Cabins), Columbus, Muscogee County, GA

  11. 15. Historic American Buildings Survey L. D. Andrew Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. Historic American Buildings Survey L. D. Andrew - Photographer June, 19, 1936 DETAIL OF NICHE AND CORNICE (RIGHT FRONT ROOM) Parlour - Blount House, (moved to Newnan vicinity, GA), Haddock, Jones County, GA

  12. 16. Historic American Buildings Survey L. D. Andrew Photographer ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. Historic American Buildings Survey L. D. Andrew - Photographer June, 19, 1936 DETAIL OF NICHE AND CORNICE (RIGHT FRONT ROOM) Parlour - Blount House, (moved to Newnan vicinity, GA), Haddock, Jones County, GA

  13. 7. Historic American Buildings Survey L. D. Andrew, Photographer Aug. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey L. D. Andrew, Photographer Aug. 26, 1936 DETAIL OF NICHE AND ARCH OVER MANTEL IN EAST FRONT ROOM - MANTEL REMOVED - Boykin Hall, Milledgeville, Baldwin County, GA

  14. 2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 VIEW FROM MORRISON'S CAF? (CORNER OF WHITAKER & CONGRESS) SHOWING S. FACADE - Gibbons Block, Congress, Saint Julian, Barnard, Whitaker Streets, Savannah, Chatham County, GA

  15. 3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 30, 1936 VIEW FROM CORNER OF WHITAKER AND ST JULIAN SHOWING NORTH FACADE - Gibbons Block, Congress, Saint Julian, Barnard, Whitaker Streets, Savannah, Chatham County, GA

  16. 9. HISTORIC AMERICAN BUILDINGS SURVEY L. D. ANDREW PHOTOGRAPHER ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    9. HISTORIC AMERICAN BUILDINGS SURVEY L. D. ANDREW - PHOTOGRAPHER SEPT. 7, 1936 BEAM, CORNICE, AND CEILING ORNAMENT IN FRONT HALL - Sorrel-Weed House, 6 West Harris Street, Savannah, Chatham County, GA

  17. 6. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 1936 From Photographs from Misses Baber-Blackshear, Macon, Georgia ARCH AND STAIRHALL - Baber House, Walnut Street, Macon, Bibb County, GA

  18. 1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged from picture photographed by Harold Bush-Brown Aug. 1936 VIEW OF FRONT AND RIGHT SIDE - Old Methodist Church, Roswell, Fulton County, GA

  19. 2. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photo From photo of Miss Edith Johnston, Savannah, Ga. 1936 VIEW OF FRONT AND RIGHT SIDE (Restoration 1936) - Wild Heron Plantation, Little Ogeechee River Vicinity, Savannah, Chatham County, GA

  20. 1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer Enlarged Photo From Photo of Miss Edith Johnston's, Savannah, Ga. 1936 VIEW OF FRONT AND SIDE (Before Restoration, 1936). - Wild Heron Plantation, Little Ogeechee River Vicinity, Savannah, Chatham County, GA

  1. 1. Historic American Buildings Survey L. D. Andrew, Photographer (Enlarged ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey L. D. Andrew, Photographer (Enlarged by) Aug. 6, 1936 Photographed by Harold Bush-Brown SIDE VIEW - Covered Bridge, Spanning Soap Creek, Atlanta, Fulton County, GA

  2. 2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. Historic American Buildings Survey L. D. Andrew, Photographer Dec. 1936 From Photograph furnished by W. Elliott Dunwody, Jr. Arch't., Macon, Ga. ENTRANCE STOOP AND DOORWAY - Baber House, Walnut Street, Macon, Bibb County, GA

  3. The Andrews-Sellers family of partition congruences.

    PubMed

    Paule, Peter; Radu, Cristian-Silviu

    2012-06-20

    In 1994, James Sellers conjectured an infinite family of Ramanujan type congruences for 2-colored Frobenius partitions introduced by George E. Andrews. These congruences arise modulo powers of 5. In 2002 Dennis Eichhorn and Sellers were able to settle the conjecture for powers up to 4. In this article, we prove Sellers' conjecture for all powers of 5. In addition, we discuss why the Andrews-Sellers family is significantly different from classical congruences modulo powers of primes. PMID:23471147

  4. A ligation-independent cloning method using nicking DNA endonuclease.

    PubMed

    Yang, Jie; Zhang, Zhihong; Zhang, Xin A; Luo, Qingming

    2010-11-01

    Using nicking DNA endonuclease (NiDE), we developed a novel technique to clone DNA fragments into plasmids. We created a NiDE cassette consisting of two inverted NiDE substrate sites sandwiching an asymmetric four-base sequence, and NiDE cleavage resulted in 14-base single-stranded termini at both ends of the vector and insert. This method can therefore be used as a ligation-independent cloning strategy to generate recombinant constructs rapidly. In addition, we designed and constructed a simple and specific vector from an Escherichia coli plasmid back-bone to complement this cloning method. By cloning cDNAs into this modified vector, we confirmed the predicted feasibility and applicability of this cloning method. PMID:21091446

  5. NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells

    SciTech Connect

    Shoji, Wataru; Suenaga, Yusuke; Kaneko, Yoshiki; Islam, S.M. Rafiqul; Alagu, Jennifer; Yokoi, Sana; Nio, Masaki; Nakagawara, Akira

    2015-06-05

    NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas. - Highlights: • NCYM promotes cleavages of MYC and MYCN to produce Myc-nick in vitro. • NCYM increases Myc-nick production in MYCN-amplified neuroblastoma cells. • NCYM knockdown decreases Myc-nick production and induces apoptosis at G2/M phase.

  6. Appreciating Unity in Diversity: An Interview with Andrew Solomon

    ERIC Educational Resources Information Center

    Peters, Dane L.

    2014-01-01

    The theme of the AMS 2014 Annual Conference is "Unity in Diversity," a concept that also describes the work of conference keynote speaker Andrew Solomon. Solomon is a writer and lecturer on psychology and politics; winner of the National Book Award; and an activist for LGBT [lesbian, gay, bisexual, and transgender] rights, mental health,…

  7. Nick Ransford: 'There is a job to do so let's get on with it'.

    PubMed

    Ransford, Nick; Doherty, Ruth

    2014-03-01

    Nick Ransford is a consultant in special care dentistry at Birmingham Community Healthcare NHS Trust. He has over 25 years' experience working with adults with disabilities and medical conditions across the spectrum. PMID:24603240

  8. PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2BGlu2

    PubMed Central

    Grundy, Gabrielle J.; Polo, Luis M.; Zeng, Zhihong; Rulten, Stuart L.; Hoch, Nicolas C.; Paomephan, Pathompong; Xu, Yingqi; Sweet, Steve M.; Thorne, Alan W.; Oliver, Antony W.; Matthews, Steve J.; Pearl, Laurence H.; Caldecott, Keith W.

    2016-01-01

    PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break. PMID:27530147

  9. Characterization of a novel eukaryal nick-sealing RNA ligase from Naegleria gruberi

    PubMed Central

    Unciuleac, Mihaela-Carmen; Shuman, Stewart

    2015-01-01

    The proteome of the amoebo-flagellate protozoan Naegleria gruberi is rich in candidate RNA repair enzymes, including 15 putative RNA ligases, one of which, NgrRnl, is a eukaryal homolog of Deinococcus radiodurans RNA ligase, DraRnl. Here we report that purified recombinant NgrRnl seals nicked 3′-OH/5′-PO4 duplexes in which the 3′-OH strand is RNA. It does so via the “classic” ligase pathway, entailing reaction with ATP to form a covalent NgrRnl–AMP intermediate, transfer of AMP to the nick 5′-PO4, and attack of the RNA 3′-OH on the adenylylated nick to form a 3′–5′ phosphodiester. Unlike members of the four known families of ATP-dependent RNA ligases, NgrRnl lacks a carboxy-terminal appendage to its nucleotidyltransferase domain. Instead, it contains a defining amino-terminal domain that we show is important for 3′-OH/5′-PO4 nick-sealing and ligase adenylylation, but dispensable for phosphodiester synthesis at a preadenylylated nick. We propose that NgrRnl, DraRnl, and their homologs from diverse bacteria, viruses, and unicellular eukarya comprise a new “Rnl5 family” of nick-sealing ligases with a signature domain organization. PMID:25740837

  10. The isolation of strand-specific nicking endonucleases from a randomized SapI expression library

    PubMed Central

    Samuelson, James C.; Zhu, Zhenyu; Xu, Shuang-yong

    2004-01-01

    The Type IIS restriction endonuclease SapI recognizes the DNA sequence 5′-GCTCTTC-3′ (top strand by convention) and cleaves downstream (N1/N4) indicating top- and bottom-strand spacing, respectively. The asymmetric nature of DNA recognition presented the possibility that one, if not two, nicking variants might be created from SapI. To explore this possibility, two parallel selection procedures were designed to isolate either top-strand nicking or bottom-strand nicking variants from a randomly mutated SapI expression library. These procedures take advantage of a SapI substrate site designed into the expression plasmid, which allows for in vitro selection of plasmid clones possessing a site-specific and strand-specific nick. A procedure designed to isolate bottom-strand nicking enzymes yielded Nb.SapI-1 containing a critical R420I substitution near the end of the protein. The top-strand procedure yielded several SapI variants with a distinct preference for top-strand cleavage. Mutations present within the selected clones were segregated to confirm a top-strand nicking phenotype for single variants Q240R, E250K, G271R or K273R. The nature of the amino acid substitutions found in the selected variants provides evidence that SapI may possess two active sites per monomer. This work presents a framework for establishing the mechanism of SapI DNA cleavage. PMID:15247348

  11. A pioneer of tropical medicine worldwide: Andrew Balfour, of Khartoum

    PubMed Central

    Adeel, Ahmed A. A.

    2013-01-01

    This is an archival account of the career of Sir Andrew Balfour in Khartoum, Sudan during the period 1902 to 1913. As the first director of the Wellcome Tropical Research Laboratories in Khartoum during the period, Andrew Balfour was tasked with establishing the laboratories and at the same time he was engaged in founding the health services in Khartoum. Balfour worked in close collaboration and support from Henry Wellcome and Reginald Wingate, the Governor General of the Sudan. The energetic and meticulous sanitary work of Balfour had a remarkable impact, with Khartoum declared mosquito-free by 1910. Establishing a research base in the laboratories was met with many challenges but eventually Balfour managed to recruit a team of dedicated researchers and to produce well-circulated publications in tropical medicine. Balfour’s work in Khartoum later lead him to a distinguished career in tropical medicine. In 1923 he was appointed the first Director of London School of Hygiene and Tropical Medicine. He was also elected President of the Royal Society of Tropical Medicine and Hygiene (1925–27). Sir Andrew Balfour, KCMG, CB, LL D (1873 –1931) PMID:27493361

  12. Characterization of the structure and melting of DNAs containing backbone nicks and gaps

    SciTech Connect

    Snowden-Ifft, E.A.; Wemmer, D.E. )

    1990-06-26

    A DNA molecule containing a gap (a missing phosphate) has been examined and compared to two other molecules of the same sequence, one containing a nick (a phosphorylated gap) and the other a normal duplex containing no break in the backbone. A second gapped sequence was also compared to a normal duplex of the same sequence. The molecules containing nicks or gaps were generated as dumbbell molecules, short helices closed by a loop at each end. The dumbbells were formed by the association of two hairpins with self-complementary dangling 5'-ends. Nuclear magnetic resonance was used to monitor the melting transition and to probe structural differences between molecules. Under the conditions used here no change in stability was observed upon phosphorylation of the gap. Structural changes upon phosphorylation of a gap or closure of a nick were minimal and were localized to the region immediately around the gap or nick. Two transitions can be observed as a gapped or nicked molecule melts, although the resolution of the two transitions varies with the salt concentration. At moderate to high salt (greater than or equal to 30 mM) the molecule melts essentially all at once. At low salt the two transitions occur at temperatures that differ by as much as 15 degrees C. In addition, comparison with other NMR melting studies indicates that the duplex formed by the overlap of the dangling ends of the hairpins is stabilized relative to a free duplex of the same sequence, probably by stacking onto the hairpin stem.

  13. To Nick or Not to Nick: Comparison of I-SceI Single- and Double-Strand Break-Induced Recombination in Yeast and Human Cells

    PubMed Central

    Katz, Samantha S.; Gimble, Frederick S.; Storici, Francesca

    2014-01-01

    Genetic modification of a chromosomal locus to replace an existing dysfunctional allele with a corrected sequence can be accomplished through targeted gene correction using the cell's homologous recombination (HR) machinery. Gene targeting is stimulated by generation of a DNA double-strand break (DSB) at or near the site of correction, but repair of the break via non-homologous end-joining without using the homologous template can lead to deleterious genomic changes such as in/del mutations, or chromosomal rearrangements. By contrast, generation of a DNA single-strand break (SSB), or nick, can stimulate gene correction without the problems of DSB repair because the uncut DNA strand acts as a template to permit healing without alteration of genetic material. Here, we examine the ability of a nicking variant of the I-SceI endonuclease (K223I I-SceI) to stimulate gene targeting in yeast Saccharomyces cerevisiae and in human embryonic kidney (HEK-293) cells. K223I I-SceI is proficient in both yeast and human cells and promotes gene correction up to 12-fold. We show that K223I I-SceI-driven recombination follows a different mechanism than wild-type I-SceI-driven recombination, thus indicating that the initial DNA break that stimulates recombination is not a low-level DSB but a nick. We also demonstrate that K223I I-SceI efficiently elevates gene targeting at loci distant from the break site in yeast cells. These findings establish the capability of the I-SceI nickase to enhance recombination in yeast and human cells, strengthening the notion that nicking enzymes could be effective tools in gene correction strategies for applications in molecular biology, biotechnology, and gene therapy. PMID:24558436

  14. ANDREWS MOUNTAIN, MAZOURKA, AND PAIUTE ROADLESS AREAS, CALIFORNIA.

    USGS Publications Warehouse

    McKee, Edwin H.; Schmauch, Steven W.

    1984-01-01

    On the basis of a mineral survey, local areas near and within the Andrews Mountain, Mazourka, and Paiute Roadless Areas, California have probable and substantiated mineral-resource potential. The principal metallic mineral resources in these roadless areas are gold, copper, and silver with lead, zinc, and tungsten, as lesser resources. A zone of probable resource potential for talc, graphite, and marble is identified in the Mazourka Roadless Area. Metallic mineralization occurs mostly in vein deposits in silicic and carbonate metasedimentary rocks peripheral to Mesozoic plutons and locally in granitic rocks as well. There is little promise for the occurrence of fossil fuel resources in the roadless areas.

  15. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... in this section shall be enforced by the Commanding Officer, Naval Support Activity, Panama...

  16. A Meta-Analytic Review of the MacAndrew Alcoholism Scale.

    ERIC Educational Resources Information Center

    Cooper-Hakim, Amy; Viswesvaran, Chockalingam

    2002-01-01

    Using meta analysis, examined the predictive validity of scores on the MacAndrew Alcoholism Scale (C. MacAndrew, 1965). Compared results for 161 studies with results for 63 studies using cut scores. Discusses why the use of continuous measures rather than cut scores is recommended. (SLD)

  17. The Pilgrimage of Joel Andrews: Aging in the Autobiography of a Yankee Farmer.

    ERIC Educational Resources Information Center

    Cole, Thomas R.; Premo, Terri

    1987-01-01

    Presents autobiographical writing as an untapped resource for the historical phenomenology of aging. Interprets the autobiography of Joel Andrews, a farmer who lived from the American Revolution through the Civil War. Argues that Andrews' autobiography reflects and helps accomplish his central task in old age, the religiously sanctioned transition…

  18. Carpet in Andrews High School. A Report by the Carpet Evaluation Team.

    ERIC Educational Resources Information Center

    Wallace, Morris S.; And Others

    In the spring of 1965, the Board of Trustees of Andrews Independent School District entered into a contract with the carpet evaluation team to analyze and evaluate the use of carpeting in the Andrews Public Schools, with emphasis on the senior high school. The two $5,000 grants served as the basis for paying for the expenses and professional…

  19. The beginnings of modern astronomy at the University of St Andrews

    NASA Astrophysics Data System (ADS)

    Batten, Alan H.

    2014-03-01

    Although the University of St Andrews is much older, teaching of and research in modern astronomy began there little more than sixty years ago. Their inception was strongly associated with one man, Erwin Finlay-Freundlich. Some account is given here of his work in St Andrews and the influence he had on younger generations.

  20. Purification of a DNA nicking-closing enzyme from mouse L cells.

    PubMed Central

    Tang, D

    1978-01-01

    A DNA nicking-closing enzyme has been purified from the nuclei of mouse L cells to 90% homogeneity. The denatured and reduced form of the enzyme has a molecular weight of 68,000 which is in agreement with the molecular weight of the native enzyme as determined by gel filtration and by sucrose sedimentation velocity assuming the protein is globular. Therefore, the active form of the enzyme is a monopolypeptide. Its isoelectric point is pH 4.2 +/- 0.2. The nicking-closing activity does not require a cofactor and does not involve any sulfhydryl group. The enzyme requires 0.2 M NaCl and pH in the range of 6.5-7.5 for optimal activity. Images PMID:211488

  1. Generation of supercoils in nicked and gapped DNA drives DNA unknotting and postreplicative decatenation

    PubMed Central

    Racko, Dusan; Benedetti, Fabrizio; Dorier, Julien; Burnier, Yannis; Stasiak, Andrzej

    2015-01-01

    Due to the helical structure of DNA the process of DNA replication is topologically complex. Freshly replicated DNA molecules are catenated with each other and are frequently knotted. For proper functioning of DNA it is necessary to remove all of these entanglements. This is done by DNA topoisomerases that pass DNA segments through each other. However, it has been a riddle how DNA topoisomerases select the sites of their action. In highly crowded DNA in living cells random passages between contacting segments would only increase the extent of entanglement. Using molecular dynamics simulations we observed that in actively supercoiled DNA molecules the entanglements resulting from DNA knotting or catenation spontaneously approach sites of nicks and gaps in the DNA. Type I topoisomerases, that preferentially act at sites of nick and gaps, are thus naturally provided with DNA–DNA juxtapositions where a passage results in an error-free DNA unknotting or DNA decatenation. PMID:26150424

  2. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

    PubMed Central

    Ran, F. Ann; Hsu, Patrick D.; Lin, Chie-Yu; Gootenberg, Jonathan S.; Konermann, Silvana; Trevino, Alexandro; Scott, David A.; Inoue, Azusa; Matoba, Shogo; Zhang, Yi; Zhang, Feng

    2013-01-01

    Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to specific genomic loci by a 20-nt guide sequence, which can tolerate certain mismatches to the DNA target and thereby promote undesired off-target mutagenesis. Here, we describe an approach that combines a Cas9 nickase mutant with pairs of guide RNAs to introduce targeted double-strand breaks. Given that individual nicks in the genome are repaired with high fidelity, simultaneous nicking via appropriately offset guide RNAs effectively extends the number of specifically recognized bases in the target site. We demonstrate that paired nicking can be used to reduce off-target activity by 50–1,000 fold in cell lines and facilitate gene knockout in mouse zygotes without sacrificing on-target cleavage efficiency. This versatile strategy thus enables a wide variety of genome editing applications with higher levels of specificity. PMID:23992846

  3. Evaluation of nicked human chorionic gonadotropin content in clinical specimens by a specific immunometric assay.

    PubMed

    Kovalevskaya, G; Birken, S; Kakuma, T; Schlatterer, J; O'Connor, J F

    1999-01-01

    We report the development and characterization of an IRMA for the direct measurement of nicked human chorionic gonadotropin (hCGn) in blood and urine. hCGn derived from a reference preparation of hCG used as an immunogen elicits monoclonal antibodies (mAbs) with enhanced recognition of human luteinizing hormone epitopes. The most specific assay for pregnancy hCGn is an IRMA composed of one mAb to choriocarcinoma-derived hCGn (C5) and a second mAb developed from immunization with normal-pregnancy hCGn. This assay was used to evaluate hCGn profiles in normal, in vitro fertilization, Down syndrome, and ectopic pregnancies. In all pregnancies, hCGn was usually present in much lower concentrations than the non-nicked hCG isoform. Our results suggest that some form of physical separation from the overwhelming quantities of non-nicked hCG present in clinical specimens will be required before accurate immunochemical estimations of hCGn can be made. PMID:9895340

  4. Trapping and breaking of in vivo nicked DNA during pulsed field gel electrophoresis.

    PubMed

    Khan, Sharik R; Kuzminov, Andrei

    2013-12-15

    Pulsed field gel electrophoresis (PFGE) offers a high-resolution approach to quantify chromosomal fragmentation in bacteria, measured as percentage of chromosomal DNA entering the gel. The degree of separation in pulsed field gel (PFG) depends on the size of DNA as well as various conditions of electrophoresis such as electric field strength, time of electrophoresis, switch time, and buffer composition. Here we describe a new parameter, the structural integrity of the sample DNA itself, that influences its migration through PFGs. We show that subchromosomal fragments containing both spontaneous and DNA damage-induced nicks are prone to breakage during PFGE. Such breakage at single-strand interruptions results in artifactual decrease in molecular weight of linear DNA making accurate determination of the number of double-strand breaks difficult. Although breakage of nicked subchromosomal fragments is field strength independent, some high-molecular-weight subchromosomal fragments are also trapped within wells under the standard PFGE conditions. This trapping can be minimized by lowering the field strength and increasing the time of electrophoresis. We discuss how breakage of nicked DNA may be mechanistically linked to trapping. Our results suggest how to optimize conditions for PFGE when quantifying chromosomal fragmentation induced by DNA damage. PMID:23770235

  5. PARP3 is a sensor of nicked nucleosomes and monoribosylates histone H2B(Glu2).

    PubMed

    Grundy, Gabrielle J; Polo, Luis M; Zeng, Zhihong; Rulten, Stuart L; Hoch, Nicolas C; Paomephan, Pathompong; Xu, Yingqi; Sweet, Steve M; Thorne, Alan W; Oliver, Antony W; Matthews, Steve J; Pearl, Laurence H; Caldecott, Keith W

    2016-01-01

    PARP3 is a member of the ADP-ribosyl transferase superfamily that we show accelerates the repair of chromosomal DNA single-strand breaks in avian DT40 cells. Two-dimensional nuclear magnetic resonance experiments reveal that PARP3 employs a conserved DNA-binding interface to detect and stably bind DNA breaks and to accumulate at sites of chromosome damage. PARP3 preferentially binds to and is activated by mononucleosomes containing nicked DNA and which target PARP3 trans-ribosylation activity to a single-histone substrate. Although nicks in naked DNA stimulate PARP3 autoribosylation, nicks in mononucleosomes promote the trans-ribosylation of histone H2B specifically at Glu2. These data identify PARP3 as a molecular sensor of nicked nucleosomes and demonstrate, for the first time, the ribosylation of chromatin at a site-specific DNA single-strand break. PMID:27530147

  6. A transnational conference romance: Elsie Andrews, Hildegarde Kneeland, and the Pan-Pacific Women's Association.

    PubMed

    Laurie, Alison J

    2009-01-01

    Elsie Andrews, a feminist activist from New Zealand, met Dr. Hildegarde Kneeland, a progressive economist from the United States, at the 1934 Pan-Pacific Women's Association conference in Honolulu. Andrews wrote diaries of her attendance at conferences, and in these writes openly of her attraction and romantic feelings for Kneeland, despite her own long-term domestic partnership back in New Plymouth with Muriel Kirton. This article considers the role conference romances may have played for Andrews and others in encouraging their interest in women's organizations, in the context of literature on romantic friendships and lesbianism. PMID:19830617

  7. The Structure-Specific Nicking of Small Heteroduplexes by the RAG Complex: Implications for Lymphoid Chromosomal Translocations

    PubMed Central

    Raghavan, Sathees C.; Gu, Jiafeng; Swanson, Patrick C.; Lieber, Michael R.

    2009-01-01

    During V(D)J recombination, the RAG complex binds at recombination signal sequences and creates double-strand breaks. In addition to this sequence-specific recognition of the RSS, the RAG complex has been shown to be a structure-specific nuclease, cleaving 3′ overhangs and 3′ flaps, and, more recently, 10 nucleotides (nt) bubble (heteroduplex) structures. Here, we assess the smallest size heteroduplex that core and full-length RAGs can cleave. We also test whether bubbles adjacent to a partial RSS are nicked any differently or any more efficiently than bubbles that are surrounded by random sequence. These points are important in considering what types and what size of non-B DNA structure that the RAG complex can nick, and this helps assess the role of the RAG complex in mediating lymphoid chromosomal translocations. We find that the smallest bubble nicked by the RAG complex is 3 nt, and proximity to a partial or full RSS sequence does not affect the nicking by RAGs. RAG nicking efficiency increases with the size of the heteroduplex and is only about two-fold less efficient than an RSS when the bubble is 6 nt. We consider these findings in the context of RAG nicking at non-B DNA structures in lymphoid chromosomal translocations. PMID:17307402

  8. The structure-specific nicking of small heteroduplexes by the RAG complex: implications for lymphoid chromosomal translocations.

    PubMed

    Raghavan, Sathees C; Gu, Jiafeng; Swanson, Patrick C; Lieber, Michael R

    2007-06-01

    During V(D)J recombination, the RAG complex binds at recombination signal sequences and creates double-strand breaks. In addition to this sequence-specific recognition of the RSS, the RAG complex has been shown to be a structure-specific nuclease, cleaving 3' overhangs and 3' flaps, and, more recently, 10 nucleotides (nt) bubble (heteroduplex) structures. Here, we assess the smallest size heteroduplex that core and full-length RAGs can cleave. We also test whether bubbles adjacent to a partial RSS are nicked any differently or any more efficiently than bubbles that are surrounded by random sequence. These points are important in considering what types and what size of non-B DNA structure that the RAG complex can nick, and this helps assess the role of the RAG complex in mediating lymphoid chromosomal translocations. We find that the smallest bubble nicked by the RAG complex is 3nt, and proximity to a partial or full RSS sequence does not affect the nicking by RAGs. RAG nicking efficiency increases with the size of the heteroduplex and is only about two-fold less efficient than an RSS when the bubble is 6nt. We consider these findings in the context of RAG nicking at non-B DNA structures in lymphoid chromosomal translocations. PMID:17307402

  9. ISS Update: RATS Principal Investigator Andrew Abercromby -- 08.29.12

    NASA Video Gallery

    NASA Public Affairs Officer Brandi Dean talks to the Research And Technology Studies (RATS) Principal Investigator Andrew Abercromby in the Space Vehicle Mock-Up Facility at Johnson Space Center in...

  10. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... waterline to 30°09′57.5″ N, 085°44′37″ W; then northerly to point of origin. (2) Area BA-1. The area...

  11. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... City; St. Andrews Bay; restricted areas. 334.761 Section 334.761 Navigation and Navigable Waters CORPS... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... waterline to 30°09′57.5″ N, 085°44′37″ W; then northerly to point of origin. (2) Area BA-1. The area...

  12. Free energy analysis and mechanism of base pair stacking in nicked DNA.

    PubMed

    Häse, Florian; Zacharias, Martin

    2016-09-01

    The equilibrium of stacked and unstacked base pairs is of central importance for all nucleic acid structure formation processes. The stacking equilibrium is influenced by intramolecular interactions between nucleosides but also by interactions with the solvent. Realistic simulations on nucleic acid structure formation and flexibility require an accurate description of the stacking geometry and stability and its sequence dependence. Free energy simulations have been conducted on a series of double stranded DNA molecules with a central strand break (nick) in one strand. The change in free energy upon unstacking was calculated for all ten possible base pair steps using umbrella sampling along a center-of-mass separation coordinate and including a comparison of different water models. Comparison to experimental studies indicates qualitative agreement of the stability order but a general overestimation of base pair stacking interactions in the simulations. A significant dependence of calculated nucleobase stacking free energies on the employed water model was observed with the tendency of stacking free energies being more accurately reproduced by more complex water models. The simulation studies also suggest a mechanism of stacking/unstacking that involves significant motions perpendicular to the reaction coordinate and indicate that the equilibrium nicked base pair step may slightly differ from regular B-DNA geometry in a sequence-dependent manner. PMID:27407106

  13. Real-time quantitative nicking endonuclease-mediated isothermal amplification with small molecular beacons.

    PubMed

    Xu, Wentao; Wang, Chenguang; Zhu, Pengyu; Guo, Tianxiao; Xu, Yuancong; Huang, Kunlun; Luo, Yunbo

    2016-04-21

    Techniques of isothermal amplification have recently made great strides, and have generated significant interest in the field of point-of-care detection. Nicking endonuclease-mediated isothermal amplification (NEMA) is an example of simple isothermal technology. In this paper, a real-time quantitative nicking endonuclease-mediated isothermal amplification with small molecular beacons (SMB-NEMA) of improved specificity and sensitivity is described. First, we optimized the prohibition of de novo synthesis by choosing Nt·BstNBI endonuclease. Second, the whole genome was successfully amplified with Nt·BstNBI (6 U), betaine (1 M) and trehalose (60 mM) for the first time. Third, we achieved 10 pg sensitivity for the first time after adding a small molecular beacon that spontaneously undergoes a conformational change when hybridizing to target, and the practical test validated the assay's application. The small molecular beacon has a similar melting temperature to the reaction temperature, but is approximately 10 bp shorter than the length of a traditional molecular beacon. A new threshold regulation was also established for isothermal conditions. Finally, we established a thermodynamic model for designing small molecular beacons. This multistate model is more correct than the traditional algorithm. This theoretical and practical basis will help us to monitor SMB-NEMA in a quantitative way. In summary, our SMB-NEMA method allows the simple, specific and sensitive assessment of isothermal DNA quantification. PMID:27027375

  14. Free energy analysis and mechanism of base pair stacking in nicked DNA

    PubMed Central

    Häse, Florian; Zacharias, Martin

    2016-01-01

    The equilibrium of stacked and unstacked base pairs is of central importance for all nucleic acid structure formation processes. The stacking equilibrium is influenced by intramolecular interactions between nucleosides but also by interactions with the solvent. Realistic simulations on nucleic acid structure formation and flexibility require an accurate description of the stacking geometry and stability and its sequence dependence. Free energy simulations have been conducted on a series of double stranded DNA molecules with a central strand break (nick) in one strand. The change in free energy upon unstacking was calculated for all ten possible base pair steps using umbrella sampling along a center-of-mass separation coordinate and including a comparison of different water models. Comparison to experimental studies indicates qualitative agreement of the stability order but a general overestimation of base pair stacking interactions in the simulations. A significant dependence of calculated nucleobase stacking free energies on the employed water model was observed with the tendency of stacking free energies being more accurately reproduced by more complex water models. The simulation studies also suggest a mechanism of stacking/unstacking that involves significant motions perpendicular to the reaction coordinate and indicate that the equilibrium nicked base pair step may slightly differ from regular B-DNA geometry in a sequence-dependent manner. PMID:27407106

  15. Trapping of DNA topoisomerase I on nick-containing DNA in cell free extracts of Saccharomyces cerevisiae.

    PubMed

    Lebedeva, Natalia; Auffret Vander Kemp, Patricia; Bjornsti, Mary-Ann; Lavrik, Olga; Boiteux, Serge

    2006-07-13

    The aim of the present study was to identify proteins that bind nicked DNA intermediates formed in the course of base excision repair (BER) in cell free extracts of Saccharomyces cerevisiae. In mammalian cells, nicks in DNA are targets of proteins such as PARP-1 or XRCC1 that have no homologues in yeast. One of the most promising methodologies to trap proteins that interact with damaged DNA lies in using a photocrosslinking technique with photoactivable dNTP analogues such as exo-N-{2-[N-(4-azido-2,5-difluoro-3-chloropyridine-6-yl)-3-aminopropionyl]-aminoethyl}-2'-deoxycytidine-5'-triphosphate (FAP-dCTP) for enzymatic synthesis of DNA probes with a photoreactive dNMP residue at the 3'-margin of a nick. Using this approach, we identified a major covalent DNA-protein adduct between a nick-containing 34-mer DNA duplex and a protein of a molecular mass of around 100-kDa. Unexpectedly, the formation of the 100-kDa adduct did not require the incorporation of the photoreactive dNMP residue at the 3'-margin of the nick nor exposure to near UV-light. However, the formation of the 100-kDa adduct strictly required a nick or a short gap in the DNA probe. Furthermore, the 100-kDa adduct was not detected in yeast extracts lacking DNA topoisomerase I (Top1). To further establish the nature of crosslinked protein, yeast Top1 was tagged with a Myc-epitope. In this case, the mobility of the Top1-DNA adduct increased by 7- kDa. Therefore, our data speak in favor of Top1 trapping by nicked DNA. In support of this hypothesis, purified yeast Top1 was also crosslinked to nicked DNA structures. Undamaged, uracil- and abasic (AP) site-containing DNAs were unable to trap Top1 under the same assay conditions. Since nicked DNA structures are frequently formed in the course of BER, their covalent linkage to Top1 has the potential to interfere with BER in vivo. PMID:16713756

  16. Rep-mediated nicking of the adeno-associated virus origin requires two biochemical activities, DNA helicase activity and transesterification.

    PubMed

    Brister, J R; Muzyczka, N

    1999-11-01

    The single-stranded adeno-associated virus (AAV) genome is flanked by terminal hairpinned origins of DNA replication (terminal repeats [TRs]) that are nicked at the terminal resolution site (trs) by the AAV Rep protein in an ATP-dependent, site-specific manner. Here we determine the minimal trs sequence necessary for Rep cleavage, 3'-CCGGT/TG-5', and show that this 7-base core sequence is required only on the nicked strand. We also identify a potential stem-loop structure at the trs. Interestingly, Rep nicking on a TR substrate that fixes this trs stem-loop in the extruded form no longer requires ATP. This suggests that ATP-dependent Rep helicase activity is necessary to unwind the duplex trs and extrude the stem-loop structure, prior to the ATP-independent Rep transesterification reaction. The extrusion of origin stem-loop structures prior to nicking appears to be a general mechanism shared by plant and animal viruses and bacterial plasmids. In the case of AAV, this mechanism of TR nicking would provide a possible regulatory function. PMID:10516041

  17. The McAndrews Leadership Lecture: February 2015, by Dr Scott Haldeman. Challenges of the Past, Challenges of the Present

    PubMed Central

    Haldeman, Scott; McAndrews, George P.; Goertz, Christine; Sportelli, Louis; Hamm, Anthony W.; Johnson, Claire

    2015-01-01

    The McAndrews Leadership Lecture was developed by the American Chiropractic Association to honor the legacy of Jerome F. McAndrews, DC, and George P. McAndrews, JD, and their contributions to the chiropractic profession. This article is a transcription of the presentation made by Dr Scott Haldeman on February 28, 2015, in Washington, DC, at the National Chiropractic Leadership Conference. PMID:26770177

  18. Hurricane Andrew's impact on natural gas and oil facilities on the outer continental shelf (interim report as of November 1993)

    SciTech Connect

    Daniels, G.R.

    1994-01-01

    The interim report reviews Hurricane Andrew's impact on Federal Outer Continental Shelf (OCS) natural gas and oil drilling and production facilities. The report provides background on Hurricane Andrew's progression, discusses how OCS operators responded to the storm, summarizes the types of damage to offshore facilies caused by Hurricane Andrew, and discusses Minerals Management Service's continuing damage assessment and repair efforts. The summaries of damage estimates are presented in tables in Appendix 1. A glossary of report terminology is provided in Appendix 2.

  19. Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA)

    PubMed Central

    Chauleau, Mathieu; Shuman, Stewart

    2016-01-01

    Escherichia coli DNA ligase (EcoLigA) repairs 3′-OH/5′-PO4 nicks in duplex DNA via reaction of LigA with NAD+ to form a covalent LigA-(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the nick 5′-PO4 to form an AppDNA intermediate (step 2); and attack of the nick 3′-OH on AppDNA to form a 3′-5′ phosphodiester (step 3). A distinctive feature of EcoLigA is its stimulation by ammonium ion. Here we used rapid mix-quench methods to analyze the kinetic mechanism of single-turnover nick sealing by EcoLigA–AMP. For substrates with correctly base-paired 3′-OH/5′-PO4 nicks, kstep2 was fast (6.8–27 s−1) and similar to kstep3 (8.3–42 s−1). Absent ammonium, kstep2 and kstep3 were 48-fold and 16-fold slower, respectively. EcoLigA was exquisitely sensitive to 3′-OH base mispairs and 3′ N:abasic lesions, which elicited 1000- to >20000-fold decrements in kstep2. The exception was the non-canonical 3′ A:oxoG configuration, which EcoLigA accepted as correctly paired for rapid sealing. These results underscore: (i) how EcoLigA requires proper positioning of the nick 3′ nucleoside for catalysis of 5′ adenylylation; and (ii) EcoLigA's potential to embed mutations during the repair of oxidative damage. EcoLigA was relatively tolerant of 5′-phosphate base mispairs and 5′ N:abasic lesions. PMID:26857547

  20. Kinetic mechanism and fidelity of nick sealing by Escherichia coli NAD+-dependent DNA ligase (LigA).

    PubMed

    Chauleau, Mathieu; Shuman, Stewart

    2016-03-18

    Escherichia coli DNA ligase (EcoLigA) repairs 3'-OH/5'-PO4 nicks in duplex DNA via reaction of LigA with NAD(+) to form a covalent LigA-(lysyl-Nζ)-AMP intermediate (step 1); transfer of AMP to the nick 5'-PO4 to form an AppDNA intermediate (step 2); and attack of the nick 3'-OH on AppDNA to form a 3'-5' phosphodiester (step 3). A distinctive feature of EcoLigA is its stimulation by ammonium ion. Here we used rapid mix-quench methods to analyze the kinetic mechanism of single-turnover nick sealing by EcoLigA-AMP. For substrates with correctly base-paired 3'-OH/5'-PO4 nicks, kstep2 was fast (6.8-27 s(-1)) and similar to kstep3 (8.3-42 s(-1)). Absent ammonium, kstep2 and kstep3 were 48-fold and 16-fold slower, respectively. EcoLigA was exquisitely sensitive to 3'-OH base mispairs and 3' N:abasic lesions, which elicited 1000- to >20000-fold decrements in kstep2. The exception was the non-canonical 3' A:oxoG configuration, which EcoLigA accepted as correctly paired for rapid sealing. These results underscore: (i) how EcoLigA requires proper positioning of the nick 3' nucleoside for catalysis of 5' adenylylation; and (ii) EcoLigA's potential to embed mutations during the repair of oxidative damage. EcoLigA was relatively tolerant of 5'-phosphate base mispairs and 5' N:abasic lesions. PMID:26857547

  1. Design and analysis of site-specific single-strand nicking endonucleases for gene correction.

    PubMed

    Metzger, Michael J; Certo, Michael T

    2014-01-01

    Single-strand nicking endonucleases ("nickases") have been shown to induce homology-mediated gene correction with reduced toxicity of DNA double-strand break-producing enzymes, and nickases have been engineered from both homing endonuclease and FokI-based scaffolds. We describe the strategies used to engineer these site-specific nickases as well as the in vitro methods used to confirm their activity and specificity. Additionally, we describe the Traffic Light Reporter system, which uses a flow cytometric assay to simultaneously detect both gene repair and mutagenic nonhomologous end-joining outcomes at a single targeted site in mammalian cells. With these methods, novel nickases can be designed and tested for use in gene correction with novel target sites. PMID:24557907

  2. Isothermal amplification detection of nucleic acids by a double-nicked beacon.

    PubMed

    Shi, Chao; Zhou, Meiling; Pan, Mei; Zhong, Guilin; Ma, Cuiping

    2016-03-01

    Isothermal and rapid amplification detection of nucleic acids is an important technology in environmental monitoring, foodborne pathogen detection, and point-of-care clinical diagnostics. Here we have developed a novel method of isothermal signal amplification for single-stranded DNA (ssDNA) detection. The ssDNA target could be used as an initiator, coupled with a double-nicked molecular beacon, to originate amplification cycles, achieving cascade signal amplification. In addition, the method showed good specificity and strong anti-jamming capability. Overall, it is a one-pot and isothermal strand displacement amplification method without the requirement of a stepwise procedure, which greatly simplifies the experimental procedure and decreases the probability of contamination of samples. With its advantages, the method would be very useful to detect nucleic acids in point-of-care or field use. PMID:26706801

  3. Lessons from isolable nickel(I) precursor complexes for small molecule activation.

    PubMed

    Yao, Shenglai; Driess, Matthias

    2012-02-21

    Small-molecule activation by transition metals is essential to numerous organic transformations, both biological and industrial. Creating useful metal-mediated activation systems often depends on stabilizing the metal with uncommon low oxidation states and low coordination numbers. This provides a redox-active metal center with vacant coordination sites well suited for interacting with small molecules. Monovalent nickel species, with their d(9) electronic configuration, are moderately strong one-electron reducing agents that are synthetically attractive if they can be isolated. They represent suitable reagents for closing the knowledge gap in nickel-mediated activation of small molecules. Recently, the first strikingly stable dinuclear β-diketiminate nickel(I) precursor complexes were synthesized, proving to be suitable promoters for small-molecule binding and activation. They have led to many unprecedented nickel complexes bearing activated small molecules in different reduction stages. In this Account, we describe selected achievements in the activation of nitrous oxide (N(2)O), O(2), the heavier chalcogens (S, Se, and Te), and white phosphorus (P(4)) through this β-diketiminatonickel(I) precursor species. We emphasize the reductive activation of O(2), owing to its promise in oxidation processes. The one-electron-reduced O(2) activation product, that is, the corresponding β-diketiminato-supported Ni-O(2) complex, is a genuine superoxonickel(II) complex, representing an important intermediate in the early stages of O(2) activation. It selectively acts as an oxygen-atom transfer agent, hydrogen-atom scavenger, or both towards exogenous organic substrates to yield oxidation products. The one-electron reduction of the superoxonickel(II) moiety was examined by using elemental potassium, β-diketiminatozinc(II) chloride, and β-diketiminatoiron(I) complexes, affording the first heterobimetallic complexes featuring a [NiO(2)M] subunit (M is K, Zn, or Fe). Through

  4. Development of a nicking endonuclease-assisted method for the purification of minicircles.

    PubMed

    Alves, Cláudia P A; Šimčíková, Michaela; Brito, Liliana; Monteiro, Gabriel A; Prazeres, Duarte Miguel F

    2016-04-22

    Minicircle (MC) DNA vectors are able to generate a high-level transgene expression in vivo, which is superior to the one afforded by conventional plasmids. MC vectors are produced by replicating a parental plasmid (PP) and promoting its recombination in Escherichia coli. This generates a MC with the expression cassette, and a miniplasmid (MP) with the replication segment. Unfortunately, wider use of MC vectors is hampered by difficulties in isolating the target MCs from their MP counterpart. In this proof-of-concept study, a reproducible process is described to improve the purification of supercoiled (sc) MCs that combines an in vitro enzymatic relaxation of sc MP impurities with topoisomer separation and RNA clearance by hydrophobic interaction chromatography (HIC) step. At the early stage of vector design, a site for the nicking endonuclease Nb.BbvCI was strategically placed in the MP part of the PP backbone. A process was then established that involves E. coli culture and recombination of PPs into target MC, cell harvesting and alkaline lysis, precipitation with isopropanol and ammonium sulfate and diafiltration/concentration by microfiltration. Next, an in vitro digestion step was carried out with Nb.BbvCI to nick of one of the strands of the MPs and of non-recombined PPs by Nb.BbvCI. As a result, sc MPs and non-recombined PPs were converted into the corresponding open circular (oc) forms whereas sc MCs remain unaffected. Finally, sc MC was isolated from oc DNA molecules (oc MPs, oc MC) and RNA by performing HIC with a phenyl-Sepharose column using a series of elution steps with decreasing ammonium sulfate concentrations. On the basis of agarose gel electrophoresis analysis, the sc MC-containing fractions were determined to be virtually free from nucleic acid impurities. PMID:27016116

  5. Andrew Sexton Gray (1826-1907). A founder of Australian ophthalmology: his life and times.

    PubMed

    Lowe, R F

    1985-11-01

    Andrew Sexton Gray was born in Limerick, Ireland, medically trained in Dublin, and was assistant to William Wilde, the distinguished oculist and aurist. He migrated to Victoria in 1859, was surgeon to a railway's construction company, then in 1862 began practice as a surgeon and oculist in Melbourne. In 1863 he founded a charitable eye and ear hospital, and had a very active, long life devoted mostly to ophthalmology. The hospital progressively expanded and became the centre for training for many ophthalmologists, as well as the nucleus for the cohesion of Victorian ophthalmology. History shows Andrew Sexton Gray to have been a founder of Australian ophthalmology. PMID:3914312

  6. A quantitative study of the flexibility contributed to RNA structures by nicks and single-stranded gaps.

    PubMed Central

    Cohen, S B; Cech, T R

    1998-01-01

    Disulfide crosslinking via thiol-disulfide interchange was applied to quantitate the relative flexibility contributed by nicks and single-stranded gaps in an RNA structure. An RNA duplex comprised of three strands was constructed containing the disulfide crosslink precursors 1 and 2 at opposite ends of the duplex on opposite strands. The third strand was of varying length to yield a nick or single-stranded gaps of 1, 2, or 3 nt. Crosslinking rates Indicated relative flexibilities of the resulting two-helix junctions. Crosslinking in the nicked duplex occurred two orders of magnitude slower than in a duplex containing a 3-nt gap. Rates of crosslinking in duplexes with 3-and 2-nt gaps showed only modest dependence on the gap sequence. Many natural RNAs, including ribozymes, contain two-helix junctions related to the model system described here. The data suggest that two-helix junctions containing a nick in one strand will retain substantial rigidity, whereas one or more single-stranded nucleotides at a two-helix junction allow significant flexibility. PMID:9769093

  7. Assistant DNA recycling with nicking endonuclease and molecular beacon for signal amplification using a target-complementary arched structure.

    PubMed

    Gao, Fenglei; Lei, Jianping; Ju, Huangxian

    2013-05-11

    A simple and universal method for ultrasensitive "signal on" detection of DNA was developed with a target-complementary arched structure to release assistant DNA, which was recycled with nicking endonuclease to amplify the detectable fluorescent signal of molecular beacons. PMID:23563493

  8. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  9. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  10. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  11. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  12. 33 CFR 334.760 - Naval Support Activity Panama City and Alligator Bayou, a tributary of St. Andrew Bay, Fla...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... City and Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. 334.760 Section... Alligator Bayou, a tributary of St. Andrew Bay, Fla.; naval restricted area. (a) The area. The waters within... the south side of the entrance to Alligator Bayou; thence directly across the entrance to a point...

  13. Astronauts Mario Runco, Jr. and Andrew S. W. Thomas, both mission specialists, pose for photo while

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-77 ESC VIEW --- Astronauts Mario Runco, Jr. and Andrew S. W. Thomas, both mission specialists, pose for photo while in the mid-deck of the Earth-orbiting Space Shuttle Endeavour. The scene was recorded with an Electronic Still Camera (ESC).

  14. 78 FR 78349 - Andrew Peklo III; Notice of Availability of Draft Environmental Assessment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-26

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Andrew Peklo III; Notice of Availability of Draft Environmental Assessment... Commission's (Commission) regulations, 18 CFR Part 380 (Order No. 486, 52 FR 47897), the Office of...

  15. Visions and Vanities: John Andrew Rice of Black Mountain College. Southern Biography Series.

    ERIC Educational Resources Information Center

    Reynolds, Katherine Chaddock

    This biography presents the life of John Andrew Rice, who founded Black Mountain College (North Carolina) in 1933 to implement his philosophy of education, including the centrality of artistic experience and emotional development to learning in all disciplines and the need for democratic governance shared between faculty and students. Born in…

  16. Commentary on Andrew Coulson's "Comparing Public, Private, and Market Schools: The International Evidence"

    ERIC Educational Resources Information Center

    Wolfram, Gary; Coulson, Andrew J.

    2009-01-01

    In this article, the authors comment on Andrew Coulson's "Comparing Public, Private, and Market Schools: The International Evidence." The authors believe that Coulson's paper is a very interesting review of the literature on the ability of market-produced education to outperform government-produced education. Coulson's response on this commentary…

  17. 77 FR 2968 - Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-01-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF ENERGY Federal Energy Regulatory Commission Pomperaug Hydro Project, Andrew Peklo III; Notice Establishing Deadline for Comments and Reply Comments On December 15, 2011, the Commission issued notice that Office of Energy Projects staff will hold a site visit...

  18. Because Everyone Has a Story to Tell: Interview with Andrew Wright

    ERIC Educational Resources Information Center

    Floris, Flora Debora

    2016-01-01

    This article presents an interview with Andrew Wright, a widely recognized author, illustrator, storyteller, and teacher trainer. Wright has published many ELT books, authored six "Spellbinder" graded readers (1992-1994), and a collection of short stories. As a teacher trainer, Wright worked extensively with both teachers and students in…

  19. Amos Kendall's Role in the Election of Andrew Jackson as President of the United States, 1828.

    ERIC Educational Resources Information Center

    Nowell, Bob

    Amos Kendall's place in journalism history rests largely on his service as a journalist turned government official in the two administrations of President Andrew Jackson. Historians have claimed that Kendall was an influential journalist of the "partisan press" era, but they have provided little documentation. That documentation has been provided…

  20. Traversing the Gap: Andrew Wright, John Hick and Critical Religious Education

    ERIC Educational Resources Information Center

    Teece, Geoff

    2005-01-01

    This paper discusses aspects of Andrew Wright's version of a liberal, critical religious education and his criticisms of some other views of modern religious education. This is attempted not by examining these "other views" as such but by concentrating on the work of John Hick. The reason for this is that Wright, like Cooling (in his book "A…

  1. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Naval Support Activity Panama... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... in this section shall be enforced by the Commanding Officer, Naval Support Activity, Panama...

  2. 33 CFR 334.761 - Naval Support Activity Panama City; St. Andrews Bay; restricted areas.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Naval Support Activity Panama... REGULATIONS § 334.761 Naval Support Activity Panama City; St. Andrews Bay; restricted areas. (a) The areas—(1... in this section shall be enforced by the Commanding Officer, Naval Support Activity, Panama...

  3. Athena, Andrew and Stanford: A Look at Implementation and Evaluation in Three Large Projects.

    ERIC Educational Resources Information Center

    Isaacs, Geoff

    1989-01-01

    Describes implementation, support, and evaluation of computer assisted learning (CAL) projects at three universities: Project Athena at Massachusetts Institute of Technology; the Andrew network at Carnegie Mellon University; and a project at Stanford University. Topics discussed include work stations, microcomputers, computer networks, graphics,…

  4. 75 FR 41895 - Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers From Andrews...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-19

    ... notice was published in the Federal Register on March 5, 2010 (75 FR 10321). The notice was amended on... published on the Federal Register on June 7, 2010 (75 FR 32221). At the request of the petitioners, the... Workers From Andrews International, Inc., M.H. Equipment, and Kenco Logistics Services, LLC,...

  5. 22. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. View of Andrew McLean Company Textile Mill looking north; Building No. 1 at left, Building No. 4 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  6. 23. View of open firststory passageway of Andrew McLean Company ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    23. View of open first-story passageway of Andrew McLean Company Textile Mill Building No. 1, looking west - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  7. 16. View of Andrew McLean Company Textile Mill looking southeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    16. View of Andrew McLean Company Textile Mill looking southeast; Building No. 6 at center, roof of Building No. 1 at left - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  8. 14. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    14. View of Andrew McLean Company Textile Mill looking north; Building No. 6 at right, Building No. 1 in background, gateposts in foreground - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  9. 20. View of Andrew McLean Company Textile Mill looking southeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    20. View of Andrew McLean Company Textile Mill looking southeast; Building No. 2 at left, Building No. 3 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  10. 15. View of Andrew McLean Company Textile Mill looking northeast; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    15. View of Andrew McLean Company Textile Mill looking northeast; Building No. 1 at left, Building No. 6 at center - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  11. 18. View of Andrew McLean Company Textile Mill looking north; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    18. View of Andrew McLean Company Textile Mill looking north; Building No. 3 at center right, Building No. 2 at center distance, Dundee Canal at left - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  12. Astronaut Andrew M. Allen, mission commander, sets up systems for a television downlink on the

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-75 ONBOARD VIEW --- Astronaut Andrew M. Allen, mission commander, sets up systems for a television downlink on the flight deck of the Space Shuttle Columbia. Allen was joined by four other astronauts and an international payload specialist for more than 16 days of research aboard Columbia. The photograph was taken with a 70mm handheld camera.

  13. Spanish Poetry and Anglo-American Modernism: The Legacy of Andrew P. Debicki.

    ERIC Educational Resources Information Center

    Mayhew, Jonathan

    2001-01-01

    Discusses the work of Andrew Debicki, an academician with a commitment to twentieth century poetry. Considers the definition of poetic modernity, one of the central issues in the study of literature in the past century. Examines the extent to which it is possible to assimilate modern Spanish poetry to the ideals of Anglo-American poetry.…

  14. 19. View of Andrew McLean Company Textile Mill looking south; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    19. View of Andrew McLean Company Textile Mill looking south; Building No. 2 at center, Botany Mills Lanolin Retrieval plant site in foreground - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  15. 31. View looking north from Andrew McLean Company Textile Mill; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    31. View looking north from Andrew McLean Company Textile Mill; Dundee Canal at center, Botany Worsted Mills in background - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  16. 17. View looking north; Andrew McLean Company Textile Mill, Building ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    17. View looking north; Andrew McLean Company Textile Mill, Building No. 1, at right, Botany Worsted Mills at left distance - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  17. 30. View looking north from Andrew McLean Company Textile Mill; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    30. View looking north from Andrew McLean Company Textile Mill; Dundee Canal at center, Botany Worsted Mills in background - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  18. 56. Aerial view looking west; Andrew McLean Company Textile Mill ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    56. Aerial view looking west; Andrew McLean Company Textile Mill at bottom, Dundee Canal at center, Botany Worsted Mills at top - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  19. Structure and two-metal mechanism of a eukaryal nick-sealing RNA ligase

    PubMed Central

    Unciuleac, Mihaela-Carmen; Goldgur, Yehuda; Shuman, Stewart

    2015-01-01

    ATP-dependent RNA ligases are agents of RNA repair that join 3′-OH and 5′-PO4 RNA ends. Naegleria gruberi RNA ligase (NgrRnl) exemplifies a family of RNA nick-sealing enzymes found in bacteria, viruses, and eukarya. Crystal structures of NgrRnl at three discrete steps along the reaction pathway—covalent ligase-(lysyl-Nζ)–AMP•Mn2+ intermediate; ligase•ATP•(Mn2+)2 Michaelis complex; and ligase•Mn2+ complex—highlight a two-metal mechanism of nucleotidyl transfer, whereby (i) an enzyme-bound “catalytic” metal coordination complex lowers the pKa of the lysine nucleophile and stabilizes the transition state of the ATP α phosphate; and (ii) a second metal coordination complex bridges the β- and γ-phosphates. The NgrRnl N domain is a distinctively embellished oligonucleotide-binding (OB) fold that engages the γ-phosphate and associated metal complex and orients the pyrophosphate leaving group for in-line catalysis with stereochemical inversion at the AMP phosphate. The unique domain architecture of NgrRnl fortifies the theme that RNA ligases have evolved many times, and independently, by fusions of a shared nucleotidyltransferase domain to structurally diverse flanking modules. The mechanistic insights to lysine adenylylation gained from the NgrRnl structures are likely to apply broadly to the covalent nucleotidyltransferase superfamily of RNA ligases, DNA ligases, and RNA capping enzymes. PMID:26512110

  20. Standard and AEGIS nicking molecular beacons detect amplicons from the Middle East respiratory syndrome coronavirus.

    PubMed

    Yaren, Ozlem; Glushakova, Lyudmyla G; Bradley, Kevin M; Hoshika, Shuichi; Benner, Steven A

    2016-10-01

    This paper combines two advances to detect MERS-CoV, the causative agent of Middle East Respiratory Syndrome, that have emerged over the past few years from the new field of "synthetic biology". Both are based on an older concept, where molecular beacons are used as the downstream detection of viral RNA in biological mixtures followed by reverse transcription PCR amplification. The first advance exploits the artificially expanded genetic information systems (AEGIS). AEGIS adds nucleotides to the four found in standard DNA and RNA (xNA); AEGIS nucleotides pair orthogonally to the A:T and G:C pairs. Placing AEGIS components in the stems of molecular beacons is shown to lower noise by preventing unwanted stem invasion by adventitious natural xNA. This should improve the signal-to-noise ratio of molecular beacons operating in complex biological mixtures. The second advance introduces a nicking enzyme that allows a single target molecule to activate more than one beacon, allowing "signal amplification". Combining these technologies in primers with components of a self-avoiding molecular recognition system (SAMRS), we detect 50 copies of MERS-CoV RNA in a multiplexed respiratory virus panel by generating fluorescence signal visible to human eye and/or camera. PMID:27421627

  1. Photoinduced Oxidative DNA Damage Revealed by an Agarose Gel Nicking Assay: A Biophysical Chemistry Laboratory Experiment

    NASA Astrophysics Data System (ADS)

    Shafirovich, Vladimir; Singh, Carolyn; Geacintov, Nicholas E.

    2003-11-01

    Oxidative damage of DNA molecules associated with electron-transfer reactions is an important phenomenon in living cells, which can lead to mutations and contribute to carcinogenesis and the aging processes. This article describes the design of several simple experiments to explore DNA damage initiated by photoinduced electron-transfer reactions sensitized by the acridine derivative, proflavine (PF). A supercoiled DNA agarose gel nicking assay is employed as a sensitive probe of DNA strand cleavage. A low-cost experimental and computer-interfaced imaging apparatus is described allowing for the digital recording and analysis of the gel electrophoresis results. The first experiment describes the formation of direct strand breaks in double-stranded DNA induced by photoexcitation of the intercalated PF molecules. The second experiment demonstrates that the addition of the well-known electron acceptor, methylviologen, gives rise to a significant enhancement of the photochemical DNA strand cleavage effect. This occurs by an electron transfer step to methylviologen that renders the inital photoinduced charge separation between photoexcited PF and DNA irreversible. The third experiment demonstrates that the action spectrum of the DNA photocleavage matches the absorption spectrum of DNA-bound, intercalated PF molecules, which differs from that of free PF molecules. This result demonstrates that the photoinduced DNA strand cleavage is initiated by intercalated rather than free PF molecules.

  2. Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of top-strand nicking variants from BsrDI and BtsI.

    PubMed

    Xu, Shuang-Yong; Zhu, Zhenyu; Zhang, Penghua; Chan, Siu-Hong; Samuelson, James C; Xiao, Jianping; Ingalls, Debra; Wilson, Geoffrey G

    2007-01-01

    BsrDI and BtsI restriction endonucleases recognize and cleave double-strand DNA at the sequences GCAATG (2/0) and GCAGTG (2/0), respectively. We have purified and partially characterized these two enzymes, and analyzed the genes that encode them. BsrDI and BtsI are unusual in two respects: each cleaves DNA as a heterodimer of one large subunit (B subunit) and one small subunit (A subunit); and, in the absence of their small subunits, the large subunits behave as sequence-specific DNA nicking enzymes and only nick the bottom strand of the sequences at these respective positions: GCAATG (-/0) and GCAGTG (-/0). We refer to the single subunit, the bottom-strand nicking forms as 'hemidimers'. Amino acid sequence comparisons reveal that BsrDI and BtsI belong to a family of restriction enzymes that possess two catalytic sites: a canonical PD-X(n)-EXK and a second non-canonical PD-X(n)-E-X12-QR. Interestingly, the other family members, which include BsrI (ACTGG 1/-1) and BsmI/Mva1269I (GAATGC 1/-1) are single polypeptide chains, i.e. monomers, rather than heterodimers. In BsrDI and BtsI, the two catalytic sites are found in two separate subunits. Site-directed mutagenesis confirmed that the canonical catalytic site located at the N-terminus of the large subunit is responsible for the bottom-strand cleavage, whereas the non-canonical catalytic site located in the small subunit is responsible for hydrolysis of the top strand. Top-strand specific nicking variants, Nt.BsrDI and Nt.BtsI, were successfully engineered by combining the catalytic-deficient B subunit with wild-type A subunit. PMID:17586812

  3. Nick translation of HeLa cell nuclei as a probe for locating DNase I-sensitive nucleosomes

    SciTech Connect

    Javaherian, K.; Fasman, G.D.

    1984-03-10

    The technique of nick translation of nuclei has been used in HeLa cells to label DNase I-sensitive regions. Micrococcal nuclease digestion of the nick translated nuclei was followed by a low ionic strength gel electrophoresis system which separates different types of mononucleosomes. The major label was observed in the vicinity of high mobility group protein containing mononucleosomes. However, further analysis revealed that the particle does not sediment in the position of mononucleosomes on a sucrose gradient. Two alternative explanations are discussed as the possible source of this particle. It is either a high mobility group protein containing nucleosome in some unfolded conformation or the labeled particle originates from discrete DNA fragments, wrapped around some nonhistone proteins, located in a highly DNase I-sensitive region, which is resistant to micrococcal nuclease digestion. 36 references, 7 figures.

  4. Nicking enzyme and graphene oxide-based dual signal amplification for ultrasensitive aptamer-based fluorescence polarization assays.

    PubMed

    Huang, Yong; Liu, Xiaoqian; Zhang, Liangliang; Hu, Kun; Zhao, Shulin; Fang, Baizong; Chen, Zhen-Feng; Liang, Hong

    2015-01-15

    In this work, two different configurations for novel amplified fluorescence polarization (FP) aptasensors based on nicking enzyme signal amplification (NESA) and graphene oxide (GO) enhancement have been developed for ultrasensitive and selective detection of biomolecules in homogeneous solution. One approach involves the aptamer-target binding induced the stable hybridization between an aptamer probe and a fluorophore-labeled DNA probe linked to GO, and forms a nicking site-containing duplex DNA region due to the enhancement of base stacking. The second analytical method involves the target induced the assembly of two aptamer subunits into an aptamer-target complex, and then hybridizes with a fluorophore-labeled DNA probe linked to GO, forming a nicking site-containing duplex DNA region. The formation of the duplex DNA region in both methods triggers the NESA process, resulting in the release of many short DNA fragments carrying the fluorophore from GO, generating a significant decrease of the FP value that provides the readout signal for the amplified sensing process. By using the NESA coupled GO enhancement path, the sensitivity of the developed aptasensors can be significantly improved by four orders of magnitude over traditional aptamer-based homogeneous assays. Moreover, these aptasensors also exhibit high specificity for target molecules, which are capable of detecting target molecule in biological samples. Considering these qualities, the proposed FP aptasensors based NESA and GO enhancement can be expected to provide an ultrasensitive platform for amplified analysis of target molecules. PMID:25087158

  5. MGME1 processes flaps into ligatable nicks in concert with DNA polymerase γ during mtDNA replication.

    PubMed

    Uhler, Jay P; Thörn, Christian; Nicholls, Thomas J; Matic, Stanka; Milenkovic, Dusanka; Gustafsson, Claes M; Falkenberg, Maria

    2016-07-01

    Recently, MGME1 was identified as a mitochondrial DNA nuclease with preference for single-stranded DNA (ssDNA) substrates. Loss-of-function mutations in patients lead to mitochondrial disease with DNA depletion, deletions, duplications and rearrangements. Here, we assess the biochemical role of MGME1 in the processing of flap intermediates during mitochondrial DNA replication using reconstituted systems. We show that MGME1 can cleave flaps to enable efficient ligation of newly replicated DNA strands in combination with POLγ. MGME1 generates a pool of imprecisely cut products (short flaps, nicks and gaps) that are converted to ligatable nicks by POLγ through extension or excision of the 3'-end strand. This is dependent on the 3'-5' exonuclease activity of POLγ which limits strand displacement activity and enables POLγ to back up to the nick by 3'-5' degradation. We also demonstrate that POLγ-driven strand displacement is sufficient to generate DNA- but not RNA-flap substrates suitable for MGME1 cleavage and ligation during replication. Our findings have implications for RNA primer removal models, the 5'-end processing of nascent DNA at OriH, and DNA repair. PMID:27220468

  6. MGME1 processes flaps into ligatable nicks in concert with DNA polymerase γ during mtDNA replication

    PubMed Central

    Uhler, Jay P.; Thörn, Christian; Nicholls, Thomas J.; Matic, Stanka; Milenkovic, Dusanka; Gustafsson, Claes M.; Falkenberg, Maria

    2016-01-01

    Recently, MGME1 was identified as a mitochondrial DNA nuclease with preference for single-stranded DNA (ssDNA) substrates. Loss-of-function mutations in patients lead to mitochondrial disease with DNA depletion, deletions, duplications and rearrangements. Here, we assess the biochemical role of MGME1 in the processing of flap intermediates during mitochondrial DNA replication using reconstituted systems. We show that MGME1 can cleave flaps to enable efficient ligation of newly replicated DNA strands in combination with POLγ. MGME1 generates a pool of imprecisely cut products (short flaps, nicks and gaps) that are converted to ligatable nicks by POLγ through extension or excision of the 3′-end strand. This is dependent on the 3′-5′ exonuclease activity of POLγ which limits strand displacement activity and enables POLγ to back up to the nick by 3′-5′ degradation. We also demonstrate that POLγ-driven strand displacement is sufficient to generate DNA- but not RNA-flap substrates suitable for MGME1 cleavage and ligation during replication. Our findings have implications for RNA primer removal models, the 5′-end processing of nascent DNA at OriH, and DNA repair. PMID:27220468

  7. Optimization of a DNA Nicking Assay to Evaluate Oenocarpus bataua and Camellia sinensis Antioxidant Capacity

    PubMed Central

    Leba, Louis-Jérôme; Brunschwig, Christel; Saout, Mona; Martial, Karine; Vulcain, Emmanuelle; Bereau, Didier; Robinson, Jean-Charles

    2014-01-01

    This study was aimed at assessing the DNA damage protective activity of different types of extracts (aqueous, methanolic and acetonic) using an in vitro DNA nicking assay. Several parameters were optimized using the pUC18 plasmid, especially FeSO4, EDTA, solvent concentrations and incubation time. Special attention has been paid to removing the protective and damaging effect of the solvent and FeSO4 respectively, as well as to identifying the relevant positive and negative controls. For each solvent, the optimal conditions were determined: (i) for aqueous extracts, 0.33 mM of FeSO4 and 0.62 mM of EDTA were incubated for 20 min at 37 °C; (ii) for acetone extracts, 1.16% solvent were incubated for 15 min at 37 °C with 1.3 mM of FeSO4 and 2.5 mM of EDTA and (iii) for methanol extracts, 0.16% solvent, were incubated for 1.5 h at 37 °C with 0.33 mM of FeSO4 and 0.62 mM of EDTA. Using the optimized conditions, the DNA damage protective activity of aqueous, methanolic and acetonic extracts of an Amazonian palm berry (Oenocarpus bataua) and green tea (Camellia sinensis) was assessed. Aqueous and acetonic Oenocarpus bataua extracts were protective against DNA damage, whereas aqueous, methanolic and acetonic extracts of Camellia sinensis extracts induced DNA damage. PMID:25302614

  8. Analyzing after-action reports from Hurricanes Andrew and Katrina: repeated, modified, and newly created recommendations.

    PubMed

    Knox, Claire Connolly

    2013-01-01

    Thirteen years after Hurricane Andrew struck Homestead, FL, Hurricane Katrina devastated the Gulf Coast of Mississippi, Alabama, and southeastern Louisiana. Along with all its destruction, the term "catastrophic" was redefined. This article extends the literature on these hurricanes by providing a macrolevel analysis of The Governor's Disaster Planning and Response Review Committee Final Report from Hurricane Andrew and three federal after-action reports from Hurricane Katrina, as well as a cursory review of relevant literature. Results provide evidence that previous lessons have not been learned or institutionalized with many recommendations being repeated or modified. This article concludes with a discussion of these lessons, as well as new issues arising during Hurricane Katrina. PMID:24180096

  9. Impact of an extreme event on the sediment budget: Hurricane Andrew in the Louisiana barrier islands

    USGS Publications Warehouse

    List, Jeffrey H.; Hansen, Mark E.; Sallenger,, Asbury H., Jr.; Jaffe, Bruce E.

    1997-01-01

    This paper examines the influence of Hurricane Andrew on the sediment budget of an 80-kilometer section of the Louisiana barrier islands west of the modern Mississippi delta. Because long-term bathymetric change has been extensively studied in this area, excellent baseline data are available for evaluating the impact of Hurricane Andrew. Results show that despite the high intensity of the storm and a storm track optimally positioned to impact the study area, the storm did not have an overwhelming influence on the sediment budget when compared to the changes occurring over the previous 50 years. For the Louisiana barrier islands, a 50-year record appears to be adequate for averaging the long-term contributions of both major and minor storm events to the sediment budget.

  10. 21. View of Andrew McLean Company Textile Mill looking west; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    21. View of Andrew McLean Company Textile Mill looking west; Building No. 4 at left, Building No. 1 at left center, Building No. 3 at center, Building No. 2 at right center, Building No. 5 at right - Dundee Canal Industrial Historic District, Beginning at George Street in Passaic & extending north along Dundee Canal approximately 1.2 miles to Canal headgates opposite East Clifton Avenue in Clifton, Passaic, Passaic County, NJ

  11. Andrew Wyeth and N.C. Wyeth: a psychodynamic perspective on father and son.

    PubMed

    Shaw, Jon A

    2013-01-01

    This paper examines the relationship between two extraordinary artists, father and son--N.C. Wyeth (1882-1945) and Andrew Wyeth (1917-2009)--and their art. N.C. Wyeth, the father, the most famous illustrator of his day, painted scenes full of drama and action, often of men engaged in violent life and death struggles. N.C. was unable to separate from his powerful mother and yearned for his iconic father. He thought himself an artistic failure and dedicated himself to raising his children to be geniuses. The youngest son, Andrew Wyeth, who lived a "secret life," painted scenes often characterized by pathos: bleak and barren landscapes, leaden skies, tire tracks, gray framed houses, desiccated fields, and circling buzzards. In the father-son relationship, we often seen three themes perpetuated developmentally: (1) the son's identification with the innermost conflicts of his father; (2) the yearning for the iconic father of his youth; and (3) a continuation and disavowal of his father's life. These themes are played out in the relationship between Andrew Wyeth and his father. PMID:23965259

  12. 78 FR 6173 - Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-01-29

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF TRANSPORTATION Surface Transportation Board Diana Del Grosso, Ray Smith, Joseph Hatch, Cheryl Hatch, Kathleen... Smith, Joseph Hatch, Cheryl Hatch, Kathleen Kelley, Andrew Wilklund, and Richard Kosiba...

  13. Kinetic mechanism of nick sealing by T4 RNA ligase 2 and effects of 3′-OH base mispairs and damaged base lesions

    PubMed Central

    Chauleau, Mathieu; Shuman, Stewart

    2013-01-01

    T4 RNA ligase 2 (Rnl2) repairs 3′-OH/5′-PO4 nicks in duplex nucleic acids in which the broken 3′-OH strand is RNA. Ligation entails three chemical steps: reaction of Rnl2 with ATP to form a covalent Rnl2–(lysyl-Nζ)–AMP intermediate (step 1); transfer of AMP to the 5′-PO4 of the nick to form an activated AppN– intermediate (step 2); and attack by the nick 3′-OH on the AppN– strand to form a 3′–5′ phosphodiester (step 3). Here we used rapid mix-quench methods to analyze the kinetic mechanism and fidelity of single-turnover nick sealing by Rnl2–AMP. For substrates with correctly base-paired 3′-OH nick termini, kstep2 was fast (9.5 to 17.9 sec−1) and similar in magnitude to kstep3 (7.9 to 32 sec−1). Rnl2 fidelity was enforced mainly at the level of step 2 catalysis, whereby 3′-OH base mispairs and oxoguanine, oxoadenine, or abasic lesions opposite the nick 3′-OH elicited severe decrements in the rate of 5′-adenylylation and relatively modest slowing of the rate of phosphodiester synthesis. The exception was the noncanonical A:oxoG base pair, which Rnl2 accepted as a correctly paired end for rapid sealing. These results underscore (1) how Rnl2 requires proper positioning of the 3′-terminal ribonucleoside at the nick for optimal 5′-adenylylation and (2) the potential for nick-sealing ligases to embed mutations during the repair of oxidative damage. PMID:24158792

  14. Wireless Andrew.

    ERIC Educational Resources Information Center

    Fickes, Michael

    2000-01-01

    Describes the use of the Internet and laptops help Carnegie Mellon University students carry out sophisticated research anywhere on campus. How the university became a wireless community is discussed. (GR)

  15. A comment on Andrew C. Papanicolaou's "Beyond Eddington's argument": pragmatism in scientific knowledge and perception.

    PubMed

    Kono, Tetsuya

    2015-03-01

    I agree with Andrew C. Papanicolaou's claim that human beings' cognitive ability can reach to the real world, but don't agree with his claim that the order of this world is akin to our thought. In this commentary paper, I will first defend a recent pragmatic scientific realism called as "entity realism" or "referential realism" which affirms that we are able to reach the real world not by inference but by technological, engineering settings. In the second half of this paper, I will also affirm that our perceptual ability directly reaches the reality too, in referring to the ecological psychology of James J. Gibson. PMID:24986382

  16. Management of Oro-Nasal Fistula Using Andrew's Bridge: A Case Report.

    PubMed

    Harish, P V; Bhojaraju, Nandakishore; Sowmya, G R; Gangaiah, Makam

    2014-09-01

    Oro-nasal fistula is the most common complication following the surgical closure of the cleft palate. Retention is the paramount factor in the successful prosthodontic habilitation of cleft palate patients. Various precision attachments have provided us with the opportunity to make the prosthesis fixed removable type; giving a double advantage to the patient i.e. comfort through fixed type and easy maintenance through removal type. This case report describes a case of oro-nasal fistula habilitated with an obturator attached to Andrew's bridge, which had good retention and esthetics. PMID:25183920

  17. Astronaut Andrew S. W. Thomas, mission specialist, interrupts a Spacehab task to pose for an

    NASA Technical Reports Server (NTRS)

    1996-01-01

    STS-77 ESC VIEW --- Astronaut Andrew S. W. Thomas, mission specialist, interrupts a Spacehab task to pose for an Electronic Still Camera (ESC) snapshot inside the Spacehab Module onboard the Earth-orbiting Space Shuttle Endeavour. In upper left is the view port which crew members had used for viewing and photographing operations with the Spartan 207/Inflatable Antenna Experiment (IAE). Thomas has his hand on an aft-bulkhead-mounted locker. The Space Experiment Facility (SEF), designed and managed by the University of Alabama, is just behind his left shoulder.

  18. Structure-based functional identification of Helicobacter pylori HP0268 as a nuclease with both DNA nicking and RNase activities

    PubMed Central

    Lee, Ki-Young; Lee, Kyu-Yeon; Kim, Ji-Hun; Lee, In-Gyun; Lee, Sung-Hee; Sim, Dae-Won; Won, Hyung-Sik; Lee, Bong-Jin

    2015-01-01

    HP0268 is a conserved, uncharacterized protein from Helicobacter pylori. Here, we determined the solution structure of HP0268 using three-dimensional nuclear magnetic resonance (NMR) spectroscopy, revealing that this protein is structurally most similar to a small MutS-related (SMR) domain that exhibits nicking endonuclease activity. We also demonstrated for the first time that HP0268 is a nicking endonuclease and a purine-specific ribonuclease through gel electrophoresis and fluorescence spectroscopy. The nuclease activities for DNA and RNA were maximally increased by Mn2+ and Mg2+ ions, respectively, and decreased by Cu2+ ions. Using NMR chemical shift perturbations, the metal and nucleotide binding sites of HP0268 were determined to be spatially divided but close to each other. The lysine residues (Lys7, Lys11 and Lys43) are clustered and form the nucleotide binding site. Moreover, site-directed mutagenesis was used to define the catalytic active site of HP0268, revealing that this site contains two acidic residues, Asp50 and Glu54, in the metal binding site. The nucleotide binding and active sites are not conserved in the structural homologues of HP0268. This study will contribute to improving our understanding of the structure and functionality of a wide spectrum of nucleases. PMID:25916841

  19. Activation of Aryl Halides by Nickel(I) Pincer Complexes: Reaction Pathways of Stoichiometric and Catalytic Dehalogenations.

    PubMed

    Rettenmeier, Christoph A; Wenz, Jan; Wadepohl, Hubert; Gade, Lutz H

    2016-08-15

    Homolytic C-X bond cleavage of organohalides by the T-shaped nickel(I) complexes [LigNi(I)] 1 bearing the iso-PyrrMeBox ligand had been found previously to be the crucial activation step in the asymmetric hydrodehalogenation of geminal dihalides. Here, this mechanistic investigation is extended to aryl halides, which allowed a systematic study of the activation process by a combination of experimental data and density functional theory modeling. While the activation of both aryl chlorides and geminal dichlorides appears to proceed via an analogous transition state, the generation of a highly stabile nickel(II)aryl species in the reaction of the aryl chlorides for the former represents a major difference in the reactive behavior. This difference was found to have a crucial impact on the activity of these nickel pincer systems as catalysts in the dehalogenation of aryl chlorides compared to geminal dichlorides and highlights the importance of the regulatory pathways controlling the nickel(I) concentration throughout the catalysis. These results along with the identification and characterization of novel nickel(II)aryl species are presented. PMID:27483018

  20. Ecological evaluation of proposed dredged material from St. Andrew Bay, Florida

    SciTech Connect

    Mayhew, H.L.; Word, J.Q.; Kohn, N.P.; Pinza, M.R.; Karle, L.M.; Ward, J.A.

    1993-10-01

    The US Army Corps of Engineers (USACE), Mobile District, requested that the Battelle/Marine Sciences Laboratory (MSL) conduct field sampling and chemical and biological testing to determine the suitability of potential dredged material for open ocean disposal. Sediment from St. Andrew Bay was chemically characterized and evaluated for biological toxicity and bioaccumulation of contaminants. The Tier III guidance for ocean disposal testing requires tests of water column effects (following dredged material disposal), deposited sediment toxicity, and bioaccumulation of contaminants from deposited sediment (dredged material). To meet these requirements, the MSL conducted suspended-particulate-phase (SPP) toxicity tests, solid-phase toxicity tests, and bioaccumulation testing on sediment representing potential dredged material from Panama City Harbor. Physical and chemical characterization of sediment to support toxicity and bioaccumulation results was also conducted on both the test and reference sediments. The MSL collected sediment samples from five sites in St. Andrew Bay and one reference site near Lands End Peninsula. The five test sediments and the reference sediment were analyzed for physical and chemical sediment characteristics, SPP chemical contaminants, solid-phase toxicity, SPP toxicity, and bioaccumulation of contaminants.

  1. ANDREWES'S CHRISTMAS FAIRY TALE: ATYPICAL THINKING ABOUT CANCER AETIOLOGY IN 1935.

    PubMed

    Sankaran, Neeraja; van Helvoort, Ton

    2016-06-20

    This paper uses a short 'Christmas fairy-story for oncologists' sent by Christopher Andrewes with a 1935 letter to Peyton Rous as the centrepiece of a reflection on the state of knowledge and speculation about the viral aetiology of cancer in the 1930s. Although explicitly not intended for public circulation at the time, the fairy-story merits publication for its significance in the history of ideas about viruses, which are taken for granted today. Andrewes and Rous were prominent members of the international medical research community and yet faced strong resistance to their theory that viruses could cause such tumours as chicken sarcomas and rabbit papillomas. By looking at exchanges between these men among themselves and other proponents of their theories and with their oncologist detractors, we highlight an episode in the behind-the-scenes workings of medical science and show how informal correspondence helped keep alive a vital but then heterodox idea about the role of viruses in causing cancer. PMID:27386716

  2. Delayed tree mortality in the Atchafalaya Basin of Southern Louisiana following Hurricane Andrew

    USGS Publications Warehouse

    Keeland, B.D.; Gorham, L.E.

    2009-01-01

    Hurricanes can damage trees in forested wetlands, and the potential for mortality related to these storms exists due to the effects of tree damage over time. In August 1992, Hurricane Andrew passed through the forested wetlands of southern Louisiana with winds in excess of 225 kph. Although more than 78 of the basal area was destroyed in some areas, most trees greater than 2.5 cm dbh were alive and resprouting prolifically the following year (98.8). Survival of most tree species was similarly high two years after the hurricane, but mortality rates of some species increased dramatically. For example, Populus heterophylla (swamp cottonwood) mortality increased from 7.8 to 59.2 (n 76) and Salix interior (sandbar willow) mortality increased from 4.5 to 57.1 (n 21). Stem sprouts on many up-rooted hardwood trees of other species were still alive in 1998, 6 years after the hurricane. Due to the understory tree species composition, regeneration, and high levels of resprouting, there was little change in species composition or perhaps a slight shift toward more shade and flood tolerant species six years following the hurricane event. Triadica sebifera (Chinese tallow) was found on some of the sites heavily disturbed by Hurricane Andrew, and may proliferate at the expense of native tree species. ?? 2009 The Society of Wetland Scientists.

  3. James Gregory, the University observatory and the early acquisition of scientific instruments at the University of St Andrews

    PubMed Central

    Rawson, Helen C.

    2015-01-01

    James Gregory, inventor of the reflecting telescope and Fellow of the Royal Society, was the first Regius Professor of Mathematics of the University of St Andrews, 1668–74. He attempted to establish in St Andrews what would, if completed, have been the first purpose-built observatory in the British Isles. He travelled to London in 1673 to purchase instruments for equipping the observatory and improving the teaching and study of natural philosophy and mathematics in the university, seeking the advice of John Flamsteed, later the first Astronomer Royal. This paper considers the observatory initiative and the early acquisition of instruments at the University of St Andrews, with reference to Gregory's correspondence, inventories made ca. 1699–ca. 1718 and extant instruments themselves, some of which predate Gregory's time. It examines the structure and fate of the university observatory, the legacy of Gregory's teaching and endeavours, and the meridian line laid down in 1748 in the University Library.

  4. Bioassay-guided isolation of novel compound from Paeonia suffruticosa Andrews roots as an IL-1β inhibitor.

    PubMed

    Choi, Yun-Hyeok; Yoo, Hee-Jung; Noh, Ill Chan; Lee, Jeong-Min; Park, Jae Won; Choi, Wahn Soo; Choi, Jung Ho

    2012-05-01

    The inhibition of Interleukin-1beta (IL-1β) is of substantial interest for the treatment of rheumatoid arthritis. Using an in vitro assay with RAW 264.7 cells, oxo-acetic acid 2-ethoxy-4-(3-hydroxy-2-oxopropyl) phenyl ester (1) was isolated from the roots of Paeonia suffruticosa Andrews as an inhibitor of IL-1β with an IC(50) value of 56 μM. Compound 1 is a novel phenylesteric compound from P. suffruticosa Andrews. Compound 1 was shown to inhibit the production of pro-inflammatory cytokines in RAW 264.7 cells. Thus, a possible new action of novel compound is provided explaining the anti-rheumatoid arthritic properties of P. suffruticosa Andrews. PMID:22644848

  5. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9.

    PubMed

    Sato, Takuya; Sakuma, Tetsushi; Yokonishi, Tetsuhiro; Katagiri, Kumiko; Kamimura, Satoshi; Ogonuki, Narumi; Ogura, Atsuo; Yamamoto, Takashi; Ogawa, Takehiko

    2015-07-14

    Mouse spermatogonial stem cells (SSCs) can be cultured for multiplication and maintained for long periods while preserving their spermatogenic ability. Although the cultured SSCs, named germline stem (GS) cells, are targets of genome modification, this process remains technically difficult. In the present study, we tested TALEN and double-nicking CRISPR/Cas9 on GS cells, targeting Rosa26 and Stra8 loci as representative genes dispensable and indispensable in spermatogenesis, respectively. Harvested GS cell colonies showed a high targeting efficiency with both TALEN and CRISPR/Cas9. The Rosa26-targeted GS cells differentiated into fertility-competent sperm following transplantation. On the other hand, Stra8-targeted GS cells showed defective spermatogenesis following transplantation, confirming its prime role in the initiation of meiosis. TALEN and CRISPR/Cas9, when applied in GS cells, will be valuable tools in the study of spermatogenesis and for revealing the genetic mechanism of spermatogenic failure. PMID:26095606

  6. A flavin-mononucleotide-binding site in Hansenula anomala nicked flavocytochrome b2, requiring the association of two domains.

    PubMed

    Gervais, M; Labeyrie, F; Risler, Y; Vergnes, O

    1980-10-01

    Previous experiments in our laboratory with Saccharomyces cervisiae flavocytochrom b2 indicated that both fragments alpha and beta of the enzyme after cleavage by yeast proteases are required to form the flavin site. More detailed experiments have not been carried out on the nicked Hansenula anomala enzyme obtained by tryptic cleavage. A method has been devised that gives a quantitative separation in 4 M urea of beta, and alpha with its heme still bound. The characteristics of the various species: isolated alpha and beta and mixed alpha + beta were studied in 4 M urea and after elimination of this reagent by dialysis in the presence of FMN and 2-mercaptoethanol. Several methods, including heme spectroscopy, tryptophan fluorescence, sedimentation studies, and titration of bound flavin, were used. The results indicate that isolated alpha and beta have a folded globular structure after renaturation. The flavin binding to the alpha + beta mixture was important (50-100%) with recovery of the flavodehydrogenase activity. In contrast, binding was not detectable (< 0.5%, Kf > 10 mM) for isolated alpha and beta. As far as mononucleotide binding is concerned, such a cooperative requirement for two folding domains has never been reported in other enzymes. The present results are discussed together with others obtained in our laboratory which demonstrate that, as deduced from their sensitivity to trypsin, the structure of S. cerevisiae and H. anomala flavocytochrome b2 protomers is triglobular 'n-x-beta' (n and x combined within alpha). The tetramer assembly, which remains intact as a nicked enzyme (alpha beta)4 after the first trypsin cleavage, is broken down following a second cleavage of the chain into four cytochrome cores (n) and a functional T-flavodehydrogenase entity, a tetramer of the type (x beta)4. PMID:7439181

  7. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 33 Navigation and Navigable Waters 3 2011-07-01 2011-07-01 false Gulf of Mexico, southeast of St... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  8. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 33 Navigation and Navigable Waters 3 2012-07-01 2012-07-01 false Gulf of Mexico, southeast of St... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  9. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 33 Navigation and Navigable Waters 3 2014-07-01 2014-07-01 false Gulf of Mexico, southeast of St... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  10. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 33 Navigation and Navigable Waters 3 2013-07-01 2013-07-01 false Gulf of Mexico, southeast of St... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  11. 33 CFR 334.680 - Gulf of Mexico, southeast of St. Andrew Bay East Entrance, small-arms firing range, Tyndall Air...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Gulf of Mexico, southeast of St... AND RESTRICTED AREA REGULATIONS § 334.680 Gulf of Mexico, southeast of St. Andrew Bay East Entrance... the Gulf of Mexico, southeast of St. Andrew Bay East Entrance within a rectangular area beginning at...

  12. Prosthetic rehabilitation of severe Siebert's Class III defect with modified Andrews bridge system.

    PubMed

    Rathee, Manu; Sikka, Neha; Jindal, Sahil; Kaushik, Ashutosh

    2015-03-01

    Prosthetic dentistry involves the replacement of missing and contiguous tissues with artificial substitutes to restore and maintain the oral functions, appearance, and health of the patient. The treatment of edentulous areas with ridge defects poses a challenging task for the dentist. Management of such cases involves a wide range of treatment options comprising mainly of surgical interventions and non surgical techniques such as use of removable, fixed or fixed- removable partial dentures. But each treatment plan undertaken should be customized according to patient needs. A variety of factors such as quality and quantity of existing contiguous hard and soft tissues, systemic condition and economic status of the patient play an important role in treatment planning, clinical outcome and prognosis. This case report presents the restoration of a Seibert's Class III ridge defect by an economical modification of Andrews Bridge in a 32 Year old patient. PMID:25821362

  13. Protocols for Biotechnological Interventions in Improvement of Vanilla (Vanilla planifolia Andrews.).

    PubMed

    Divakaran, Minoo; Babu, K Nirmal; Peter, K V

    2016-01-01

    Vanilla (Vanilla planifolia Andrews (syn. V. fragrans Salisb.), a native of Central America, is the primary source of natural vanillin and plays a major role in the global economy. The gene pool of vanilla is threatened by deforestation and overcollection that has resulted in disappearance of natural habitats and wild species. Continuous vegetative propagation and lack of natural seed set and sufficient variations in the gene pool hamper crop improvement programs. In vitro techniques, one of the key tools of plant biotechnology, can be employed for overcoming specific problems, viz. production of disease-free clones, inducing somaclonal variations, developing hybrids, gene pool conservation, incorporating desired traits by distant hybridization, genetic engineering, etc. However, realization of these objectives necessitates standardization of protocols. This chapter describes the various protocols optimized for crop improvement in Vanilla species. PMID:27108309

  14. The post-disaster negative health legacy: pregnancy outcomes in Louisiana after Hurricane Andrew.

    PubMed

    Antipova, Anzhelika; Curtis, Andrew

    2015-10-01

    Disasters and displacement increasingly affect and challenge urban settings. How do pregnant women fare in the aftermath of a major disaster? This paper investigates the effect of pregnancies in disaster situations. The study tests a hypothesis that pregnant women residing in hurricane-prone areas suffer higher health risks. The setting is Louisiana in the Gulf Coast, United States, a state that continually experiences hurricane impacts. The time period for the analysis is three years following the landfall of Hurricane Andrew in 1992. We analysed low birth weight and preterm deliveries before and after landfall, as a whole and by race. Findings support an association between hazards and health of a community and indicate that pregnant women in the affected area, irrespective of race, are more likely to experience preterm deliveries compared to pre-event births. Results suggest there is a negative health legacy impact in Louisiana as a result of hurricane landfall. PMID:25754615

  15. Comprehensive assessment of health needs 2 months after Hurricane Andrew--Dade County, Florida, 1992.

    PubMed

    1993-06-11

    On August 24, 1992, Hurricane Andrew struck southern Florida. More than 28,000 houses, mobile homes, and apartment buildings were destroyed, and approximately 107,000 additional dwellings sustained major damage. An estimated 180,000 persons were left homeless; insured damages were estimated at $15.5 billion and total damages at more than $30 billion. During the recovery period, many private and public health-care facilities damaged or destroyed in the storm were not functional. During November 3-13, to help prioritize health needs and direct public health resources, the Dade County Public Health Unit of the Florida Department of Health and Rehabilitative Services conducted a survey to assess health needs and the availability of health-care services during the recovery phase with funds provided by the Federal Emergency Management Agency (FEMA). This report summarizes the results of the survey. PMID:8502216

  16. Business closure and relocation: a comparative analysis of the Loma Prieta earthquake and Hurricane Andrew.

    PubMed

    Wasileski, Gabriela; Rodríguez, Havidán; Diaz, Walter

    2011-01-01

    The occurrence of a number of large-scale disasters or catastrophes in recent years, including the Indian Ocean tsunami (2004), the Kashmir earthquake (2005), Hurricane Katrina (2005) and Hurricane Ike (2008), have raised our awareness regarding the devastating effects of disasters on human populations and the importance of developing mitigation and preparedness strategies to limit the consequences of such events. However, there is still a dearth of social science research focusing on the socio-economic impact of disasters on businesses in the United States. This paper contributes to this research literature by focusing on the impact of disasters on business closure and relocation through the use of multivariate logistic regression models, specifically focusing on the Loma Prieta earthquake (1989) and Hurricane Andrew (1992). Using a multivariate model, we examine how physical damage to the infrastructure, lifeline disruption and business characteristics, among others, impact business closure and relocation following major disasters. PMID:20722689

  17. 77 FR 15600 - Special Local Regulation; Emerald Coast Super Boat Grand Prix; Saint Andrew Bay; Panama City, FL

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-03-16

    ...The Coast Guard is establishing a temporary special local regulation for a portion of Saint Andrew Bay, Panama City, FL. This action is necessary for the safeguard of participants and spectators, including all crews, vessels, and persons on navigable waters during the Emerald Coast Super Boat Grand Prix high speed boat races. Entry into, transiting in or anchoring in this area is prohibited to......

  18. A Comparison of Two Procedures, the Mahalanobis Distance and the Andrews-Pregibon Statistic, for Identifying Multivariate Outliers.

    ERIC Educational Resources Information Center

    Jarrell, Michele Glankler

    This repeated measures factorial design study compared the results of two procedures for identifying multivariate outliers under varying conditions, the Mahalanobis distance and the Andrews-Pregibon statistic. Results were analyzed for the total number of outliers identified and number of false outliers identified. Simulated data were limited to…

  19. A Comparison of Two Procedures, the Mahalanobis Distance and the Andrews-Pregibon Statistic, for Identifying Multivariate Outliers.

    ERIC Educational Resources Information Center

    Jarrell, Michele Glanker

    1994-01-01

    Computer simulations used to compare the Mahalanobis distance procedure with the Andrews-Pregibon statistic for identifying multivariate outliers result in the conclusion that the choice of procedure is not critical, since both identified valid data points as outliers. Results with false outliers as the dependent variable suggest the importance of…

  20. The Relationship of MacAndrew Alcoholism Scale Scores to MMPI Profile Type and Degree of Elevation.

    ERIC Educational Resources Information Center

    Pfost, Karen S.; And Others

    1984-01-01

    Investigated the relationship of the MacAndrew Alcoholism Scale (MAC) to personality type and level of emotional distress using Minnesota Multiphasic Personality Inventory scores of 38 alcoholic males. No relationship was found between the MAC and magnitude of psychological distress, as measured by T scores. (JAC)

  1. 75 FR 81637 - Endangered and Threatened Wildlife and Plants; Notice of Availability of the St. Andrew Beach...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... (63 FR 70053). The endangered St. Andrew beach mouse is now found in two populations: East Crooked... recovery plan available for public comment from April 22, 2009 through June 22, 2009 (74 FR 18403). We... feral cats and hogs in beach mouse habitat. 4. In areas with known populations of beach mice...

  2. [The Andrew Heiskell Library for the Blind and Physically Handicapped.] A Library with a Difference. Projects and Experiments.

    ERIC Educational Resources Information Center

    Jarkon, Joe; Fitzpatrick, Vicki, Ed.

    This publication describes the Andrew Heiskell Library for the Blind and Physically Handicapped, a regional library for the National Library Service (NLS) example of the creative use of physical space and innovative technology. The publication focuses on the materials-handling system designed for the new facility, including system design…

  3. Should Community College Be Free? Forum. "Education Next" Talks with Sara Goldrick-Rab and Andrew P. Kelly

    ERIC Educational Resources Information Center

    Goldrick-Rab, Sara; Kelly, Andrew P.

    2016-01-01

    In this article, "Education Next" talks with Sara Goldrick-Rab and Andrew Kelly. President Obama's proposal for tuition-free community college, seems to have laid down a marker for the Democratic Party. Vermont senator Bernie Sanders is touting his plan for free four-year public college on the primary trail; Massachusetts senator…

  4. Highly Selective and Sensitive Electrochemiluminescence Biosensor for p53 DNA Sequence Based on Nicking Endonuclease Assisted Target Recycling and Hyperbranched Rolling Circle Amplification.

    PubMed

    Yang, Linlin; Tao, Yingzhou; Yue, Guiyin; Li, Ruibao; Qiu, Bin; Guo, Longhua; Lin, Zhenyu; Yang, Huang-Hao

    2016-05-17

    An ultrasensitive and specific electrochemiluminescence (ECL) biosensor has been designed for the p53 DNA sequence, which is based on cascade signal amplification of nicking endonuclease assisted target recycling and hyperbranched rolling circle amplification (HRCA). First of all, biotin modified hairpin capture DNA (HP) probe was immobilized on the surface of streptavidin magnespheres paramagnetic particles (PMPs). Target DNA hybridized with the loop portion of the HP probe, therefore unfolding HP to form a double-stranded DNA (dsDNA) containing the specific nicking site of the nicking endonuclease. Then, the nicking endonuclease recognized the specific nicking site and cleaved the HP into two pieces, liberating target DNA and the complementary sequence piece for the padlock probe. The intact target DNA would initiate the next cycle of hybridization and cleavage, thereby releasing multiple complementary sequences for the padlock probes. The liberated complementary sequences hybridized with the padlock probes, subsequently inducing the HRCA reaction and generating numerous dsDNA segments. Herein, Ru(phen)3(2+) was embedded into dsDNA and worked as ECL signal reporter. The reaction products were eventually pretreated by dialysis tube with the cutoff membrane to remove the residual Ru(phen)3(2+) in the solution for the following ECL measurements. Using this cascade amplification strategy, an ultrasensitive p53 DNA sequence detection method was developed with a wide linear range from 0.05 to 100 fM and a low detection limit of 0.02 fM. Moreover, this cascade amplified ECL biosensor had specific recognition capacity for noncomplementary and single- and double-base mismatched DNA. The proposed ECL biosensor might have a great potential in biomedical research and clinic analysis. PMID:27086663

  5. Directional mapping of DNA nicking in ejaculated and cauda epididymidal spermatozoa of the short-beaked echidna (Tachyglossus aculeatus: Monotremata).

    PubMed

    Johnston, S D; López-Fernández, C; Gosálbez, A; Holt, W V; Gosálvez, J

    2009-01-01

    Prototherian spermatozoa are unique amongst the Mammalia in terms of their filiform morphology, tandem arrangement of chromosomes and formation of sperm bundles. In the present study, we provide observations of echidna spermatozoa and note that the superstructure of the bundle is engineered around the shape of the individual sperm head and that this in turn may be a consequence of the unusual circumferential and helicoidal condensation of the DNA during spermiogenesis. Frozen-thawed ejaculated echidna spermatozoa were incubated and examined for the presence of non-typical DNA conformation by means of in situ labelling of DNA breaks using Klenow polymerase and via alkaline single-cell comet assays for detection of fragmented DNA. Both techniques successfully revealed the presence of what appeared to be directional DNA nicking, co-localised with the presence of highly sensitive alkali sites along the length of the sperm nucleus. It was not possible to define whether these alternative DNA configurations were associated with a failure of the sperm nucleus to condense appropriately during spermiogenesis or were evidence of DNA fragmentation following post-thaw incubation or a sequential structural chromatin rearrangement necessary for fertilisation. PMID:19874725

  6. Genomewide scan for adaptive differentiation along altitudinal gradient in the Andrew's toad Bufo andrewsi.

    PubMed

    Guo, Baocheng; Lu, Di; Liao, Wen Bo; Merilä, Juha

    2016-08-01

    Recent studies of humans, dogs and rodents have started to discover the genetic underpinnings of high altitude adaptations, yet amphibians have received little attention in this respect. To identify possible signatures of adaptation to altitude, we performed a genome scan of 15 557 single nucleotide polymorphisms (SNPs) obtained with restriction site-associated DNA sequencing of pooled samples from 11 populations of Andrew's toad (Bufo andrewsi) from the edge of the Tibetan Plateau, spanning an altitudinal gradient from 1690 to 2768 m.a.s.l. We discovered significant geographic differentiation among all sites, with an average FST   = 0.023 across all SNPs. Apart from clear patterns of isolation by distance, we discovered numerous outlier SNPs showing strong associations with variation in altitude (1394 SNPs), average annual temperature (1859 SNPs) or both (1051 SNPs). Levels and patterns of genetic differentiation in these SNPs were consistent with the hypothesis that they have been subject to directional selection and reflect adaptation to altitudinal variation among the study sites. Genes with footprints of selection were significantly enriched in binding and metabolic processes. Several genes potentially related to high altitude adaptation were identified, although the identity and functional significance of most genomic targets of selection remain unknown. In general, the results provide genomic support for results of earlier common garden and low coverage genetic studies that have uncovered substantial adaptive differentiation along altitudinal and latitudinal gradients in amphibians. PMID:27289071

  7. Simulation of Water Balance and Forest Treatment Effects at the H.J. Andrews Experimental Forest

    SciTech Connect

    Waichler, Scott R.; Wemple, Beverley C.; Wigmosta, Mark S.

    2005-10-30

    The watershed model DHSVM was applied to the small watersheds WS1,2,3 in H.J. Andrews Experimental Forest (HJA), Oregon and tested for skill in simulating observed forest treatment effects on streamflow. These watersheds in the rain-snow transition zone underwent road and clearcut treatments during 1959-66 and subsequent natural regeneration. DHSVM was applied with 10 m and 1 hr resolution to 1958-98, most of the period of record. Water balance for old-growth WS2 indicated that evapotranspiration and streamflow were unlikely to be the only loss terms, and groundwater recharge was included to account for about 12% of precipitation; this term was assumed zero in previous studies. After limited calibration, overall efficiency in simulating hourly streamflow exceeded 0.7, and mean annual error was less than 10%. Model skill decreased at the margins, with overprediction of low flows and underprediction of high flows. However, statistical analyses of simulated and observed peakflows yielded similar characterizations of treatment effects. Primary simulation weaknesses were snowpack accumulation, snowmelt under rain-on-snow conditions, and production of quickflow. This challenging test of DHSVM moved the model closer to a practical tool for forest management.

  8. Storm-tide elevations produced by Hurricane Andrew along the southern Florida coasts, August 24, 1992

    USGS Publications Warehouse

    Murray, Mitchell H.

    1994-01-01

    On August 24, 1992, Hurricane Andrew crossed southern peninsular Florida. The combined effects of storm surge from the hurricane and astronomical tide, referred to as storm tide, caused flooding over a large part of southern Florida. Subsequent to the flooding, many high-water marks were identified, described, and surveyed along the south- eastern coast of Florida (Miami to Key Largo) and at selected areas along the southwestern coast of Florida (Flamingo to Goodland). Descriptions of these 336 high-water makrs are presented in tabular form in this report and their locations are plotted on nineteen 7.5-minute topographic quadrangle maps. For the southeastern coast, north-south profiles of the high-water makrs along the outher and inner barrier islands and the western shoreline of Biscayne Bay are presented. Average storm-tide elevations (relative to sea level) ranged from 4 to 6 feet in northern Biscayne Bay, were as much as 17 feet on the western shoreline near the center of the bay and ranged from 3 to 6 feet in southern Biscayne Bay and Barnes Sound. Storm-tide elevations along the southwestern coast ranged from 4 to 5 feet at Flamingo and 5 to 7 feet at Goodland in the Ten Thousand Islands area.

  9. Unsaturated flow modeling for performance assessment of a radioactive waste disposal facility in Andrews County, Texas

    NASA Astrophysics Data System (ADS)

    Martinez Baquero, G. F.; Singh, A.; Holt, R. M.; grisak, G. E.

    2011-12-01

    Quantitifying infiltration rates is a key component of the performance assessment for radioactive waste disposal facilities. In arid regions with scarce infiltration data, this is a challenging problem because of the computational limitations of available numerical implementations to solve water flow and transport equations. This work summarizes methodology and analysis performed to overcome some of these challenges and to generate infiltration scenarios for a low level waste disposal site in Andrews County, Texas. The work presented here includes preparation of a two dimensional finite element model in HYDRUS that includes the cover system and adjacent geologic units, calibration of hydraulic properties and root water uptake parameters based on soft information, preparation of atmospheric forcings based on current and hypothesized future climatic conditions, evaluation of impacts related to temporal and spatial discretization of forcings and model domain, and definition of scenarios for cover degradation and wetter climate conditions. Results of this work include a sensitivity analysis of infiltration rates to changes in boundary conditions under quasi-steady state, evaluation of the impact of temporal discretization of the atmospheric forcings in terms of water balance error and computational efficiency, and the estimation of infiltration rates under different scenarios. Infiltration rates from this work are being incorporated into a transport model to estimate potential radiological doses based on performance assessment modeling analyses. Findings from this work seek to contribute towards robust approaches to estimate infiltration in arid regions.

  10. A single catalytic domain of the junction-resolving enzyme T7 endonuclease I is a non-specific nicking endonuclease

    PubMed Central

    Guan, Chudi; Kumar, Sanjay

    2005-01-01

    A stable heterodimeric protein containing a single correctly folded catalytic domain (SCD) of T7 endonuclease I was produced by means of a trans-splicing intein system. As predicted by a model presented earlier, purified SCD protein acts a non-specific nicking endonuclease on normal linear DNA. The SCD retains some ability to recognize and cleave a deviated DNA double-helix near a nick or a strand-crossing site. Thus, we infer that the non-specific and nicked-site cleavage activities observed for the native T7 endonuclease I (as distinct from the resolution activity) are due to uncoordinated actions of the catalytic domains. The positively charged C-terminus of T7 Endo I is essential for the enzymatic activity of SCD, as it is for the native enzyme. We propose that the preference of the native enzyme for the resolution reaction is achieved by cooperativity in the binding of its two catalytic domains when presented with two of the arms across a four-way junction or cruciform structure. PMID:16264086

  11. The beyond 12/23 restriction is imposed at the nicking and pairing steps of DNA cleavage during V(D)J recombination.

    PubMed

    Drejer-Teel, Anna H; Fugmann, Sebastian D; Schatz, David G

    2007-09-01

    The beyond 12/23 (B12/23) rule ensures inclusion of a Dbeta gene segment in the assembled T-cell receptor (TCR) beta variable region exon and is manifest by a failure of direct Vbeta-to-Jbeta gene segment joining. The restriction is enforced during the DNA cleavage step of V(D)J recombination by the recombination-activating gene 1 and 2 (RAG1/2) proteins and the recombination signal sequences (RSSs) flanking the TCRbeta gene segments. Nothing is known about the step(s) at which DNA cleavage is defective or how TCRbeta locus sequences contribute to these defects. To address this, we examined the steps of DNA cleavage by the RAG proteins using TCRbeta locus V, D, and J RSS oligonucleotide substrates. The results demonstrate that the B12/23 rule is enforced through slow nicking of Jbeta substrates and to some extent through poor synapsis of Vbeta and Jbeta substrates. Nicking is controlled largely by the coding flank and, unexpectedly, the RSS spacer, while synapsis is controlled primarily by the RSS nonamer. The results demonstrate that different Jbeta substrates are crippled at different steps of cleavage by distinct combinations of defects in the various DNA elements and strongly suggest that the DNA nicking step of V(D)J recombination can be rate limiting in vivo. PMID:17636023

  12. The Beyond 12/23 Restriction Is Imposed at the Nicking and Pairing Steps of DNA Cleavage during V(D)J Recombination▿

    PubMed Central

    Drejer-Teel, Anna H.; Fugmann, Sebastian D.; Schatz, David G.

    2007-01-01

    The beyond 12/23 (B12/23) rule ensures inclusion of a Dβ gene segment in the assembled T-cell receptor (TCR) β variable region exon and is manifest by a failure of direct Vβ-to-Jβ gene segment joining. The restriction is enforced during the DNA cleavage step of V(D)J recombination by the recombination-activating gene 1 and 2 (RAG1/2) proteins and the recombination signal sequences (RSSs) flanking the TCRβ gene segments. Nothing is known about the step(s) at which DNA cleavage is defective or how TCRβ locus sequences contribute to these defects. To address this, we examined the steps of DNA cleavage by the RAG proteins using TCRβ locus V, D, and J RSS oligonucleotide substrates. The results demonstrate that the B12/23 rule is enforced through slow nicking of Jβ substrates and to some extent through poor synapsis of Vβ and Jβ substrates. Nicking is controlled largely by the coding flank and, unexpectedly, the RSS spacer, while synapsis is controlled primarily by the RSS nonamer. The results demonstrate that different Jβ substrates are crippled at different steps of cleavage by distinct combinations of defects in the various DNA elements and strongly suggest that the DNA nicking step of V(D)J recombination can be rate limiting in vivo. PMID:17636023

  13. Multi-stage impregnation of the lithospheric mantle at the Andrew Bain FZ (SWIR)

    NASA Astrophysics Data System (ADS)

    Paganelli, E.; Brunelli, D.; Bonatti, E.; Cipriani, A.; Ligi, M.

    2008-12-01

    The Southern ridge-transform intersection of Andrew Bain Fracture Zone (ABFZ) is interpreted as a "cold spot" in the mid-ocean ridge system being characterized by a negative thermal anomaly in the oceanic upper mantle. The negative thermal anomaly is associated to the cold-edge effect due to the great age contrast of the active ridge segments. During the oceanic expedition AB06-S23, in 2006, (organized by ISMAR-CNR, Bologna, Italy, and co-financed by PRNA, Italy) with the russian R/V N. Strakhov, several samples of abyssal peridotites have been collected. Textures and modal distribution of the samples have been investigated revealing a multistage impregnation history. Deep spinel-field impregnation assemblages (sp+cpx-ol) are followed by plagioclase-field patches and mineral trails (pl+cpx-ol) and late shallow gabbroic pockets and veins. The major elements mineral chemistry reveals compositional trends of low-P/T subsolidus partial- to-complete re-equilibration undergone by the upper mantle during the upwelling beneath the ridge. These samples have experienced variable degrees of melting and reacted with percolating melts of possible different composition. In particular, samples showing the lowest degrees of melting have interacted with MORB-like melts and pyroxenitic-derived melts in the spinel and plagioclase stability fields. The presence of these two kinds of melts might prove the presence of enriched portions scattered in a normal depleted mantle beneath ocean ridges. MELTS-based runs provide constraints to variable extents of pyroxenitic-derived melt interaction with the mantle source and crystallization at variable depth of the products of such an interaction. Supported by MIUR-PRIN Cofin project 2007

  14. Tests of executive functioning predict scores on the MacAndrew Alcoholism Scale.

    PubMed

    Deckel, A W

    1999-02-01

    1. Previous work reported that tests of executive functioning (EF) predict the risk of alcoholism in subject populations selected for a "high density" of a family history of alcoholism and/or the presence of sociopathic traits. The current experiment examined the ability of EF tests to predict the risk of alcoholism, as measured by the MacAndrew Alcoholism Scale (MAC), in outpatient subjects referred to a general neuropsychological testing service. 2. Sixty-eight male and female subjects referred for neuropsychological testing were assessed for their past drinking histories and administered the Wisconsin Card Sorting Test, the Wechsler Adult Intelligence Scale-Revised, the Trails (Part B) Test, and the MAC. Principal Components analysis (PCA) reduced the number of EF tests to two measures, including one that loaded on the WCST, and one that loaded on the Similarities, Picture Arrangement, and Trails tests. Multiple hierarchical regression first removed the variance from demographic variables, alcohol consumption, and verbal (i.e., Vocabulary) and non-verbal (i.e., Block Design) IQ, and then entered the executive functioning factors into the prediction of the MAC. 3. Seventy-six percent of the subjects were classified as either light, infrequent, or non-drinkers on the Quantity-Frequency-Variability scale. The factor derived from the WCST on PCA significantly added to the prediction of risk on the MAC (p = .0063), as did scores on Block Design (p = .033). Relatively more impaired scores on the WCST factor and Block Design were predictive of higher scores on the MAC. The other factors were not associated with MAC scores. 4. These results support the hypothesis that decrements in EF are associated with risk factors for alcoholism, even in populations where the density of alcoholic behaviors are not unusually high. When taken in conjunction with other findings, these results implicate EF test scores, and prefrontal brain functioning, in the neurobiology of the risk for

  15. Construction, Startup and Operation of a New LLRW Disposal Facility in Andrews County, Texas - 12151

    SciTech Connect

    Van Vliet, James A.

    2012-07-01

    During this last year, Waste Control Specialists LLC (WCS) completed construction and achieved start of operations of a new low level radioactive waste (LLRW) disposal facility in Andrews County Texas. Disposal operations are underway for commercial LLRW, and start up evolutions are in progress for disposal of Department of Energy (DOE) LLRW. The overall approach to construction and start up are presented as well as some of the more significant challenges and how they were addressed to achieve initial operations of the first new commercial low level radioactive waste disposal facility in more than 30 years. The WCS disposal facility consists of two LLRW disposal cells, one for Texas Compact waste, and a separate disposal cell for DOE waste. Both disposal cells have very robust and unique designs. The cells themselves are constructed entirely in very low permeability red bed clay. The cell liners include a 0.91 meter thick clay liner meeting unprecedented permeability limits, 0.3 meter thick reinforced concrete barriers, as well as the standard geo-synthetic liners. Actions taken to meet performance criteria and install these liners will be discussed. Consistent with this highly protective landfill design, WCS chose to install a zero discharge site water management system. The considerations behind the design and construction of this system will be presented. Other activities essential to successful start of LLRW disposal operations included process and procedure development and refinement, staffing and staff development, and training. Mock ups were built and used for important evolutions and functions. Consistent with the extensive regulation of LLRW operations, engagement with the Texas Commission on Environmental Quality (TCEQ) was continuous and highly interactive. This included daily activity conference calls, weekly coordination calls and numerous topical conference calls and meetings. TCEQ staff and consultants frequently observed specific construction

  16. On remembering and forgetting our autobiographical pasts: retrograde amnesia and Andrew Mayes's contribution to neuropsychological method.

    PubMed

    Kopelman, M D; Bright, P

    2012-11-01

    Andrew Mayes's contribution to the neuropsychology of memory has consisted in steadily teasing out the nature of the memory deficit in the amnesic syndrome. This has been done with careful attention to matters of method at all stages. This particularly applies to his investigations of forgetting rates in amnesia and to his studies of retrograde amnesia. Following a brief outline of his work, the main current theories of retrograde amnesia are considered: consolidation theory, episodic-to-semantic shift theory, and multiple trace theory. Findings across the main studies in Alzheimer dementia are reviewed to illustrate what appears to be consistently found, and what is much more inconsistent. A number of problems and issues in current theories are then highlighted--including the nature of the temporal gradient, correlations with the extent of temporal lobe damage, what we would expect 'normal' remote memory curves to look like, how they would appear in focal retrograde amnesia, and whether we can pinpoint retrograde amnesia to hippocampal/medial temporal damage on the basis of existing studies. A recent study of retrograde amnesia is re-analysed to demonstrate temporal gradients on recollected episodic memories in hippocampal/medial temporal patients. It is concluded that there are two requirements for better understanding of the nature of retrograde amnesia: (i) a tighter, Mayesian attention to method in terms of both the neuropsychology and neuroimaging in investigations of retrograde amnesia; and (ii) acknowledging that there may be multiple factors underlying a temporal gradient, and that episodic and semantic memory show important interdependencies at both encoding and retrieval. Such factors may be critical to understanding what is remembered and what is forgotten from our autobiographical pasts. PMID:22884958

  17. Low-Frequency Response Following the Passage of Hurricane Andrew on the Texas-Louisiana Shelf

    NASA Astrophysics Data System (ADS)

    Pearce, S. M.; Smith, D. C.; Dimarco, S. F.

    2009-12-01

    During August 24th through 27th in 1992, Hurricane Andrew passed through the Gulf Of Mexico almost directly over several moorings on the easternmost Louisiana shelf portion of the Texas-Louisiana Shelf (LATEX) coastal ocean monitoring program. Examination of the current meter time-series showed the existence of fast moving, long shelf waves over the entire Texas-Louisiana shelf west of the storm passage for up to 12 days after direct forcing ceased. The LATEX program featured 31 moorings each with 3 current meters over the 10, 20, 50, and 200 meter isobaths in 5 cross sectional lines with additional coverage on the 200 meter isobath from the Louisiana-Mississippi River delta, to Corpus Christi, Texas. Additionally, several pressure records from LATEX and several NOAA historical coastal tide gauge data from Sabine Pass to Port Isabella, Texas were incorporated. Raw, 3-hour low pass filtered, and 40-hour low pass filtered versions of the current data were analyzed. The pressure data used were detided using a least squares fit, and the tidal records were detided using the NOAA predicted tides for that location. All data were analyzed using a wavelet analysis to determine the spectra over time. The analyzed data shows that the shelf response was largely dominated in the internal Kelvin wave mode. The wave propagated towards the west on the shelf at approximately 400 km/day. These results are contrasted and compared with wave modes predicted for coastal trapped wave solutions. The output of a coastal ocean model simulation using a forced wind field similar to the storm are also contrasted and compared with the observed data.

  18. Cylindric partitions, {{\\boldsymbol{ W }}}_{r} characters and the Andrews-Gordon-Bressoud identities

    NASA Astrophysics Data System (ADS)

    Foda, O.; Welsh, T. A.

    2016-04-01

    We study the Andrews-Gordon-Bressoud (AGB) generalisations of the Rogers-Ramanujan q-series identities in the context of cylindric partitions. We recall the definition of r-cylindric partitions, and provide a simple proof of Borodin’s product expression for their generating functions, that can be regarded as a limiting case of an unpublished proof by Krattenthaler. We also recall the relationships between the r-cylindric partition generating functions, the principal characters of {\\hat{{sl}}}r algebras, the {{\\boldsymbol{ M }}}r r,r+d minimal model characters of {{\\boldsymbol{ W }}}r algebras, and the r-string abaci generating functions, providing simple proofs for each. We then set r = 2, and use two-cylindric partitions to re-derive the AGB identities as follows. Firstly, we use Borodin’s product expression for the generating functions of the two-cylindric partitions with infinitely long parts, to obtain the product sides of the AGB identities, times a factor {(q;q)}∞ -1, which is the generating function of ordinary partitions. Next, we obtain a bijection from the two-cylindric partitions, via two-string abaci, into decorated versions of Bressoud’s restricted lattice paths. Extending Bressoud’s method of transforming between restricted paths that obey different restrictions, we obtain sum expressions with manifestly non-negative coefficients for the generating functions of the two-cylindric partitions which contains a factor {(q;q)}∞ -1. Equating the product and sum expressions of the same two-cylindric partitions, and canceling a factor of {(q;q)}∞ -1 on each side, we obtain the AGB identities.

  19. Evaluation of Hydraulically Significant Discontinuities in Dockum Group Mudrocks in Andrews County, Texas

    NASA Astrophysics Data System (ADS)

    Holt, R. M.; Kuszmaul, J. S.; Cao, S.; Powers, D. W.

    2013-12-01

    Triassic mudrocks of the Dockum Group (Cooper Canyon Formation) host four, below-grade landfills at the Waste Control Specialists (WSC) site in Andrews County, Texas, including: a hazardous waste landfill and three radioactive waste landfills. At the study site, the Dockum consists of mudrocks with sparse siltstone/sandstone interbeds that developed in a semi-arid environment from an ephemeral meandering fluvial system. Sedimentary studies reveal that the mudrocks are ancient floodplain vertisols (soils with swelling clays) and siltstone/sandstone interbeds are fluvial channel deposits that were frequently subaerially exposed. Rock discontinuities, including fractures and syndepositional slickensided surfaces, were mapped during the excavation of the WCS radioactive waste landfills along vertical faces prepared by the construction contractor. Face locations were selected to insure a sampled area with nearly complete vertical coverage for each landfill. Individual discontinuities were mapped and their strike, dip, length, roughness, curvature, staining, and evidence of displacement were described. In the three radioactive waste disposal landfills, over 1750 discontinuities across 35 excavated faces were mapped and described, where each face was nominally 8 to 10 ft tall and 50 to 100 ft long. Genetic units related to paleosol development were identified. On average, the orientation of the discontinuities was horizontal, and no other significant trends were observed. Mapping within the landfill excavations shows that most discontinuities within Dockum rocks are horizontal, concave upward, slickensided surfaces that developed in the depositional environment, as repeated wetting and drying cycles led to shrinking and swelling of floodplain vertisols. Fractures that showed staining (a possible indicator of past or present hydraulic activity) are rare, vertical to near-vertical, and occur mainly in, and adjacent to, mechanically stiff siltstone and sandstone interbeds

  20. Effect of Hurricane Andrew on the Turkey Point Nuclear Generating Station from August 20--30, 1992. [Final report

    SciTech Connect

    Hebdon, F.J.

    1993-03-01

    On August 24, 1992, Hurricane Andrew, a Category 4 hurricane, struck the Turkey Point Electrical Generating Station with sustained winds of 145 mph (233 km/h). This is the report of the team that the US Nuclear Regulatory Commission (NRC) and the Institute of Nuclear Power Operations (INPO) jointly sponsored (1) to review the damage that the hurricane caused the nuclear units and the utility`s actions to prepare for the storm and recover from it, and (2) to compile lessons that might benefit other nuclear reactor facilities.

  1. A tale of two storms: Surges and sediment deposition from Hurricanes Andrew and Wilma in Florida’s southwest coast mangrove forests: Chapter 6G in Science and the storms-the USGS response to the hurricanes of 2005

    USGS Publications Warehouse

    Smith, Thomas J., III; Anderson, Gordon H.; Tiling, Ginger

    2007-01-01

    Hurricanes can be very different from each other. Here we examine the impacts that two hurricanes, Andrew and Wilma, had in terms of storm surge and sediment deposition on the southwest coast of Florida. Although Wilma was the weaker storm, it had the greater impact. Wilma had the higher storm surge over a larger area and deposited more sediment than did Andrew. This effect was most likely due to the size of Wilma's eye, which was four times larger than that of Andrew.

  2. The structure of new nickel(I) oxides: LnSr 5Ni 3O 8 (Ln = Y, Dy, Ho, Er and Tm)

    NASA Astrophysics Data System (ADS)

    James, M.; Attfield, J. P.

    1994-12-01

    Stoichiometric oxides of 3d 9 nickel(I), LnSr 5Ni 3O 8 (Ln = Y, Dy, Ho, Er and Tm), have been prepared through the hydrogen reduction of LnSr 5Ni 3O 11, resulting in a change from a tetragonal K 2NiF 4 type structure to an orthorhombic Sr 2CuO 3 arrangement in which {1}/{3} of the bridging oxygen atoms are missing from chains of apex-linked nickel oxide square planes.

  3. Patterns and drivers of Holocene vegetational change near the prairie-forest ecotone in Minnesota: revisiting McAndrews' transect.

    PubMed

    Nelson, David M; Hu, Feng Sheng

    2008-07-01

    Holocene vegetational dynamics along the prairie-forest border of Minnesota were first documented in McAndrews' classic work. Despite numerous subsequent paleo-studies, a number of questions remain unanswered about the vegetation history of the region. Here, pollen, stable-isotope, mineral, and charcoal data are described from three lakes near McAndrews' sites. These data were compared with other paleoenvironmental records to reconstruct vegetation, aridity, and fire. The climate was relatively wet with increasing summer temperatures before approximately 8000 yr before present (BP). The rates of changes were asymmetric for the onset and termination of middle-Holocene aridity, with an abrupt increase at approximately 8000 yr BP and a gradual, but variable, decline from approximately 7800 to 4000 yr BP. Early-Holocene coniferous forests changed to mixed-grass prairie without an intervening period of tallgrass prairie or deciduous forest, whereas the retreat of prairie was characterized by transitions from mixed-grass to tallgrass prairie to deciduous forest and finally to coniferous forest. Within the middle Holocene, the composition and structures of grass-dominated vegetation varied both temporally and spatially. Fire primarily responded to changes in climate and fuel loads. Vegetation was more strongly influenced by climatic changes than by fire-regime shifts. PMID:19086180

  4. Label-free and ratiometric detection of nuclei acids based on graphene quantum dots utilizing cascade amplification by nicking endonuclease and catalytic G-quadruplex DNAzyme.

    PubMed

    Wang, Guang-Li; Fang, Xin; Wu, Xiu-Ming; Hu, Xue-Lian; Li, Zai-Jun

    2016-07-15

    Herein, we report a ratiometric fluorescence assay based on graphene quantum dots (GQDs) for the ultrasensitive DNA detection by coupling the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for cascade signal amplifications. With o-phenylenediamine acted as the substrate of G-quadruplex/hemin DNAzyme, whose oxidization product (that is, 2,3-diaminophenazine, DAP) quenched the fluorescence intensity of GQDs (at 460nm) obviously, accompanied with the emergence of a new emission of DAP (at 564nm). The ratiometric signal variations at the emission wavelengths of 564 and 460nm (I564/I460) were utilized for label-free, sensitive, and selective detection of target DNA. Utilizing the nicking endonuclease assisted target recycling and the G-quadruplex/hemin DNAzyme biocatalysis for amplified cascade generation of DAP, the proposed bioassay exhibited high sensitivity toward target DNA with a detection limit of 30fM. The method also had additional advantages such as facile preparation and easy operation. PMID:26950646

  5. Site- and strand-specific nicking of DNA by fusion proteins derived from MutH and I-SceI or TALE repeats

    PubMed Central

    Gabsalilow, Lilia; Schierling, Benno; Friedhoff, Peter; Pingoud, Alfred; Wende, Wolfgang

    2013-01-01

    Targeted genome engineering requires nucleases that introduce a highly specific double-strand break in the genome that is either processed by homology-directed repair in the presence of a homologous repair template or by non-homologous end-joining (NHEJ) that usually results in insertions or deletions. The error-prone NHEJ can be efficiently suppressed by ‘nickases’ that produce a single-strand break rather than a double-strand break. Highly specific nickases have been produced by engineering of homing endonucleases and more recently by modifying zinc finger nucleases (ZFNs) composed of a zinc finger array and the catalytic domain of the restriction endonuclease FokI. These ZF-nickases work as heterodimers in which one subunit has a catalytically inactive FokI domain. We present two different approaches to engineer highly specific nickases; both rely on the sequence-specific nicking activity of the DNA mismatch repair endonuclease MutH which we fused to a DNA-binding module, either a catalytically inactive variant of the homing endonuclease I-SceI or the DNA-binding domain of the TALE protein AvrBs4. The fusion proteins nick strand specifically a bipartite recognition sequence consisting of the MutH and the I-SceI or TALE recognition sequences, respectively, with a more than 1000-fold preference over a stand-alone MutH site. TALE–MutH is a programmable nickase. PMID:23408850

  6. Plant phenology patterns at three sites on the H.J. Andrews Experimental Forest, Oregon, 1987 to 2007.

    NASA Astrophysics Data System (ADS)

    Shaw, D. C.; Creel, C.; Downing, G.; Remillard, S.; O'Connell, K.

    2007-12-01

    Plant phenology data has been collected at three sites on the H.J. Andrews Experimental Forest, Oregon since the late 1970's. The sites were visited once every three weeks year-round. Current efforts to clean and archive this data are on-going. Here we present on a 20 year data set from 1987 to 2007. The three sites are located at Watersheds (WS) 10, 8, and 7/6, at an elevation of 466 m, 993 m, and 905/950 m respectively. Forests were old growth (WS 8) and regenerating clearcuts (WS 6, 10), or shelterwood clearcut with overstory removed in 1984 (WS 7) dominated by Douglas-fir, western hemlock, true firs, and western redcedar. One tree (Douglas-fir), two evergreen shrubs, three deciduous shrubs, and four herbs were followed for vegetative and flowering phenology, including bud swell, bud bread, leaf expansion, leaf color change, leaf fall, flower bud swell, blooming, petal loss, fruit formation, and seed dispersal. Weather stations are located at each site and tied into a network of stations in this LTER site. Physical factors such as snow depth, snow coverage, freeze-thaw activity, as well as lichen condition were also noted. We are asking two key questions regarding plant phenology patterns. 1. Has the growing season lengthened in the mountainous watershed of the HJ Andrews, and is this similar for a low elevation site (WS 10) versus a mid elevation site (WS 8, 7/6)? 2. Do snow-pack dynamics influence plant phenology more so than temperature (degree days)?

  7. The Pedagogy and Problems of Jane Andrews's "The Seven Little Sisters Who Live on the Round Ball that Floats in the Air" (1861)

    ERIC Educational Resources Information Center

    Tedesco, Laureen

    2006-01-01

    This essay examines the interplay between Jane Andrews's purpose and her pedagogy in "The Seven Little Sisters Who Live on the Round Ball that Floats in the Air." The book demonstrates the teaching strategies she learned at the First State Normal in Massachusetts, moving from what the child knows to new material, engaging the child in personal…

  8. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  9. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  10. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  11. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  12. 33 CFR 334.770 - Gulf of Mexico and St. Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla.; restricted.... Andrew Sound, south of East Bay, Fla., Tyndall Drone Launch Corridor, Tyndall Air Force Base, Fla... referred to as the “Tyndall Drone Launch Corridor.” (b) The regulations. (1) Military usage of areas...

  13. Analysis of DNase 1 sensitivity and methylation of active and inactive X chromosomes of kangaroos (Macropus robustus) by in situ nick translation.

    PubMed

    Loebel, D A; Johnston, P G

    1993-01-01

    The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X chromosomes to be distinguished. No difference was found between the active and inactive X chromosomes with DNase I or MspI digestion. When chromosomes were digested with the methylation sensitive restriction enzymes HpaII and HhaI, the inactive X chromosome was labelled to a greater extent. These results indicate no overall difference in chromatin condensation between the active and inactive X chromosomes and greater overall methylation of the active X chromosome. This relative undermethylation of the inactive X chromosome may be important in X chromosome inactivation, but its function, if any, remains to be determined. PMID:8381740

  14. Highly sensitive fluorescence assay of DNA methyltransferase activity via methylation-sensitive cleavage coupled with nicking enzyme-assisted signal amplification.

    PubMed

    Zhao, Yongxi; Chen, Feng; Wu, Yayan; Dong, Yanhua; Fan, Chunhai

    2013-04-15

    Herein, using DNA adenine methylation (Dam) methyltransferase (MTase) as a model analyte, a simple, rapid, and highly sensitive fluorescence sensing platform for monitoring the activity and inhibition of DNA MTase was developed on the basis of methylation-sensitive cleavage and nicking enzyme-assisted signal amplification. In the presence of Dam MTase, an elaborately designed hairpin probe was methylated. With the help of methylation-sensitive restriction endonuclease DpnI, the methylated hairpin probe could be cleaved to release a single-stranded DNA (ssDNA). Subsequently, this released ssDNA would hybridize with the molecular beacon (MB) to open its hairpin structure, resulting in the restoration of fluorescence signal as well as formation of the double-stranded recognition site for nicking enzyme Nt.BbvCI. Eventually, an amplified fluorescence signal was observed through the enzymatic recycling cleavage of MBs. Based on this unique strategy, a very low detection limit down to 0.06 U/mL was achieved within a short assay time (60 min) in one step, which is superior to those of most existing approaches. Owing to the specific site recognition of MTase toward its substrate, the proposed sensing system was able to readily discriminate Dam MTase from other MTase such as M.SssI and even detect the target in complex biological matrix. Furthermore, the application of the proposed sensing strategy for screening Dam MTase inhibitors was also demonstrated with satisfactory results. This novel method not only provides a promising platform for monitoring activity and inhibition of DNA MTases, but also shows great potentials in biological process researches, drugs discovery and clinical diagnostics. PMID:23202331

  15. Label-free and ultrasensitive fluorescence detection of cocaine based on a strategy that utilizes DNA-templated silver nanoclusters and the nicking endonuclease-assisted signal amplification method.

    PubMed

    Zhang, Kai; Wang, Ke; Zhu, Xue; Zhang, Jue; Xu, Lan; Huang, Biao; Xie, Minhao

    2014-01-01

    A general and reliable strategy for the detection of cocaine was proposed utilizing DNA-templated silver nanoclusters as signal indicators and the nicking endonuclease-assisted signal amplification method. This strategy can detect cocaine specifically with a detection limit as low as 2 nM by using a small volume of 5 μL. PMID:24217291

  16. Andrew Halliday, Kt FRCPE (1781-1839): service in the Napoleonic Wars and West Indies, and first physician to the Seamen's Hospital Society.

    PubMed

    Cook, G C

    2004-08-01

    Sir Andrew Halliday (1781-1839) was domestic physician to HRH the Duke of Clarence (1765-1837) (who was later to become King William IV). He also served in the Napoleonic Wars-in Portugal, Spain and Waterloo-as well as in the West Indies, and became the first physician to the Seamen's Hospital Society. Unlike many of his contemporaries, Halliday was also a prolific author, many of his publications being on lunatic asylums in Great Britain and Ireland. PMID:15257347

  17. Validation of the sperm chromatin dispersion (SCD) test in the amphibian Xenopus laevis using in situ nick translation and comet assay.

    PubMed

    Pollock, K; Gosálvez, J; Arroyo, F; López-Fernández, C; Guille, M; Noble, A; Johnston, S D

    2015-11-01

    The integrity of sperm DNA is becoming increasingly recognised as an important parameter of semen quality, but there are no published reports of this procedure for any amphibian. The primary aim of this study was to apply a modified sperm chromatin dispersion (SCD) test (Halomax) to an amphibian sperm model (African clawed frog; Xenopus laevis) and to validate the assay against in situ nick translation (ISNT) and the double-comet assay procedure. Inactivated spermatozoa were collected from fresh testes (n=3). Sperm DNA fragmentation (SDF) for each sperm sample was conducted immediately following activation (T0) and again after 1h (T1) and 24h (T24) of incubation at room temperature in order to produce a range of spermatozoa with differing levels of DNA damage. The SCD procedure resulted in the production of three nuclear morphotypes; amphibian sperm morphotype 1 (ASM-1) and ASM-2 showed no evidence of DNA damage, whereas ASM-3 spermatozoa were highly fragmented with large halos of dispersed DNA fragments and a reduced nuclear core. ISNT confirmed that ASM-3 nuclei contained damaged DNA. There was a significant correlation (r=0.9613) between the levels of ASM-3 detected by the SCD test and SDF revealed by the double-comet assay. PMID:25482041

  18. Nicking endonuclease-assisted signal amplification of a split molecular aptamer beacon for biomolecule detection using graphene oxide as a sensing platform.

    PubMed

    Li, Xiang; Ding, Xuelian; Fan, Jing

    2015-12-01

    Sensitive and selective detection of ultralow concentrations of specific biomolecules is important in early clinical diagnoses and biomedical applications. Many types of aptasensors have been developed for the detection of various biomolecules, but usually suffer from false positive signals and high background signals. In this work, we have developed an amplified fluorescence aptasensor platform for ultrasensitive biomolecule detection based on enzyme-assisted target-recycling signal amplification and graphene oxide. By using a split molecular aptamer beacon and a nicking enzyme, the typical problem of false positive signals can be effectively resolved. Only in the presence of a target biomolecule, the sensor system is able to generate a positive signal, which significantly improves the selectivity of the aptasensor. Moreover, using graphene oxide as a super-quencher can effectively reduce the high background signal of a sensing platform. We select vascular endothelial growth factor (VEGF) and adenosine triphosphate (ATP) as model analytes in the current proof-of-concept experiments. It is shown that under optimized conditions, our strategy exhibits high sensitivity and selectivity for the quantification of VEGF and ATP with a low detection limit (1 pM and 4 nM, respectively). In addition, this biosensor has been successfully utilized in the analysis of real biological samples. PMID:26502364

  19. Ab initio DNA synthesis by Bst polymerase in the presence of nicking endonucleases Nt.AlwI, Nb.BbvCI, and Nb.BsmI.

    PubMed

    Antipova, Valeriya N; Zheleznaya, Lyudmila A; Zyrina, Nadezhda V

    2014-08-01

    In the absence of added DNA, thermophilic DNA polymerases synthesize double-stranded DNA from free dNTPs, which consist of numerous repetitive units (ab initio DNA synthesis). The addition of thermophilic restriction endonuclease (REase), or nicking endonuclease (NEase), effectively stimulates ab initio DNA synthesis and determines the nucleotide sequence of reaction products. We have found that NEases Nt.AlwI, Nb.BbvCI, and Nb.BsmI with non-palindromic recognition sites stimulate the synthesis of sequences organized mainly as palindromes. Moreover, the nucleotide sequence of the palindromes appeared to be dependent on NEase recognition/cleavage modes. Thus, the heterodimeric Nb.BbvCI stimulated the synthesis of palindromes composed of two recognition sites of this NEase, which were separated by AT-reach sequences or (A)n (T)m spacers. Palindromic DNA sequences obtained in the ab initio DNA synthesis with the monomeric NEases Nb.BsmI and Nt.AlwI contained, along with the sites of these NEases, randomly synthesized sequences consisted of blocks of short repeats. These findings could help investigation of the potential abilities of highly productive ab initio DNA synthesis for the creation of DNA molecules with desirable sequence. PMID:24965874

  20. Inhibition of the gastric H+,K+ -ATPase by plectrinone A, a diterpenoid isolated from Plectranthus barbatus Andrews.

    PubMed

    Schultz, Carla; Bossolani, Myllene P; Torres, Luce M B; Lima-Landman, Maria Teresa R; Lapa, Antonio J; Souccar, Caden

    2007-04-20

    This work assessed the mechanism underlying the antisecretory gastric acid effect of Plectranthus barbatus Andrews (Lamiaceae) and active constituents. Popularly known as "false-boldo", this plant is used in Brazilian folk medicine to treat gastrointestinal and hepatic ailments. The plant aqueous extract (AE) and isolated compounds were assayed in vivo in pylorus-ligated mice, and in vitro on acid secretion measured as [(14)C]-aminopyrine ([(14)C]-AP) accumulation in rabbit gastric glands and gastric H(+),K(+)-ATPase preparations. Injected into the duodenal lumen, the AE of the plant leaves (0.5 and 1.0 g/kg) decreased the volume (62 and 76%) and total acidity (23 and 50%) of gastric acid secretion in pylorus-ligated mice. Bioguided purification of the AE yielded an active fraction (IC(50)=24 microg/ml) that inhibited acid secretion in rabbit gastric glands with a potency 10 to 18 times greater than that of the originating extract, on both the basal and stimulated acid secretion by histamine (His) (1 microM) or bethanechol (100 microM). At the same concentrations the gastric H(+),K(+)-ATPase activity was also inhibited. The active constituent was chemically identified as the abietanoid dienedione plectrinone A which reduced the H(+),K(+)-ATPase activity with IC(50)=171 microM. The results indicate that inhibition of the gastric proton pump by this diterpenoid may account for the antisecretory acid effect and reputed anti ulcer activity of Plectranthus barbatus. PMID:17166678

  1. Once a physicist: Nick Horvath

    NASA Astrophysics Data System (ADS)

    Horvath, Nick

    2009-05-01

    How did you get into basketball? That's an easy one: I was tall. I had zero interest in sport at a young age and was pushed into basketball against my will when I was about eight. I hated it. The next year, however, I was so much taller than everyone else that in my first game, even though I had no idea what I was doing, I could simply hold the ball above my head and shoot over everyone. I fell in love with basketball after that first game. If I had played badly that day, it is likely that I would not have played again in my entire life.

  2. Hydrological Controls on Nitrogen and DOC Transport at the Plot, Hillslope and Catchment Scale, HJ Andrews Experimental Forest.

    NASA Astrophysics Data System (ADS)

    van Verseveld, W. J.; McDonnell, J. J.; Lajtha, K.

    2005-12-01

    While the flushing of nutrients at the catchment scale has been described in many forested environments during the last decade, the flushing mechanisms, flowpaths and geographic sources of different N species (DON, NO3- and NH4+) and DOC are still poorly resolved, especially during different storm size and antecedent wetness conditions. We characterized flowpaths of N and DOC at the hillslope scale during and between storm events in WS10, H.J. Andrews, Oregon, USA, for five storms over the period Fall 2004 until Spring 2005. This catchment is dominated by hillslopes with negligible riparian water storage due to 1986 and 1996 debris flows that evacuated the valley bottom. This enabled us to study the hydrological and biogeochemical coupling between the hillslope and catchment in a way unimpeded by riparian zone groundwater dynamics.Through a combination of hydrometric and chemistry data from groundwater wells, tension and zero tension lysimeters at different depths, tensiometers, soil moisture probes and hillslope runoff from a 10 meter wide trench at the hillslope, we were able to resolve the dominant flowpaths. Fluorescence (a proxy for DOC) of hillslope and catchment runoff was monitored continuously with a fluorometer during storms. Preliminary data analysis showed a significant relationship between DOC concentrations and fluorescence values suggesting that fluorescence can be used to characterize DOC dynamics at small time scales. Our high frequency DOC characterization showed a clockwise hystersis pattern of DOC and total N against discharge for both hillslope and catchment runoff. This suggests flushing of nutrients in near and/ or in stream zones during the initial part of the storm. Total N and DOC concentrations in groundwater wells and lysimeters at shallow soil depths were high compared to other potential sources during storms. Our interpretation is that vertical preferential flow of high concentration water drives the groundwater contribution to

  3. Nomina nova in Platyhelminthes pro Macrorhynchus von Graff, 1882 (non [Gmelin, 1801]; non Dunker, 1843), and Leptocleidus Mueller, 1936 (non Andrews, 1922).

    PubMed

    Hornung, Jahn J

    2016-01-01

    Two genus-group names of flat-worms-Leptocleidus Mueller, 1936 and Macrorhynchus von Graff, 1882-are junior homonyms that are preoccupied by fossil diapsid reptile genera-Leptocleidus Andrews, 1922, and Macrorhynchus Dunker, 1843-and an extant teleost fish genus-Macrorhynchus [Gmelin, 1801] ex La Cépède, 1800. These are replaced by nomina nova (Pharyngodytes nom. nov.; Graffiellus nom. nov.). Macrorhynchus [Gmelin, 1801] is an objective senior synonym of Macrorhyncus Dumeríl, 1805 ex La Cépède, 1800 (syn. nov.), and a senior homonym of Macrorhynchus Dunker, 1843, and Macrorhynchus von Graff, 1882. PMID:27615836

  4. New monoterpene glycosides from Paeonia suffruticosa Andrews and their inhibition on NO production in LPS-induced RAW 264.7 cells.

    PubMed

    Ding, Liqin; Zhao, Feng; Chen, Lixia; Jiang, Zhihu; Liu, Yue; Li, Zhuomin; Qiu, Feng; Yao, Xinsheng

    2012-12-01

    Nine new monoterpene glycosides (1-9), together with 18 known compounds were obtained from the n-butanol soluble fraction of ethanol extract from Paeonia suffruticosa Andrews. Their structures were determined on the basis of chemical methods and spectroscopic data. The inhibitory effects of these compounds (except compound 6) on nitric oxide (NO) production in lipopolysaccharide-activated macrophages were evaluated. Compounds 1, 21, 23, 25, and 27 showed strong inhibitory activity on NO production with IC(50) values of 31.25, 36.13, 12.41, 6.87, and 41.94 μM, respectively. PMID:23067550

  5. Tavern patrons and the MacAndrew Alcoholism Scale: self-reported drinking behavior in relation to the MMPI L and K scales.

    PubMed

    Ciancio, C; Saltstone, R; Fraboni, M

    1990-09-01

    This study investigated the ability of the MacAndrew Alcoholism (MAC) Scale to distinguish between alcoholics and nonalcoholics using self-ratings from patrons of taverns (N = 128); while only about 14% of the variance in the MAC Scale was accounted for by the self-rating (alcoholic/nonalcoholic) scale, the MAC Scale correctly identified 70% of those who reported alcohol use to levels consistent with alcoholic dependency after variance in the alcohol use scale attributable to the L and K scales of the MMPI was accounted for. PMID:2246380

  6. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    ScienceCinema

    Moniz, Ernest [U.S. Energy Secretary

    2014-08-22

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  7. Hurricane Frederic tidal floods of September 12-13, 1979, along the Gulf Coast, Pine Beach, St. Andrews Bay and Fort Morgan quadrangles, Alabama

    USGS Publications Warehouse

    Scott, John C.; Bohman, Larry R.

    1980-01-01

    Shown on a topographic map are floodmark elevations and approximate areas flooded by Hurricane Frederic tides of September 12-13, 1979, along the shores of St. Andrews Bay, Mobile Bay, and Bon Secour Bay from Fort Morgan eastward to about four miles east of Gasque, Ala. The storm tide went completely across the land between the beach and Mobile Bay throughout much of the area. Most homes on the beach side of Alabama State Highway 180 were completely destroyed, and the highway was washed out in several places. Damage to homes and other structures on the bay side was not as great. Storm-tide frequency and records of annual maximum tides at Mobile, Ala., since 1772, are presented. Offshore winds reached about 160 miles per hour. A wind-velocity of about 145 miles per hour was recorded near Dauphin Island, Ala. (USGS)

  8. The work of Andrew Weil and Deepak Chopra--two holistic health/New Age gurus: a critique of the holistic health/New Age movements.

    PubMed

    Baer, Hans A

    2003-06-01

    Despite the popular roots of the holistic health/New Age movements, a growing number of biomedical physicians have become proponents of holistic health as well as New Age healing. Over the past two decades, Andrew Weil and Deepak Chopra, two biomedically trained physicians, have emerged as the visible and financially successful spokespersons of the movement. This article provides brief biographical sketches of Weil and Chopra and compares and contrasts their respective views on health, illness, healing, and health care. It also considers the response of various biomedical parties to these holistic health/New Age gurus who have attempted to integrate biomedicine and various alternative healing and metaphysical systems. Finally, this article argues that Weil and Chopra both epitomize the limitations of the holistic health/New Age movements, albeit in different ways. PMID:12846118

  9. Observations on some of the plates used to illustrate the Lymphatics section of Andrew Fyfe's Compendium of the Anatomy of the Human Body, Published in 1800.

    PubMed

    Kaufman, M H

    1999-01-01

    An engraving displaying A General View of the Absorbent System was used as one of many plates illustrating the Lymphatics section of Andrew Fyfe's Compendium of the Anatomy of the Human Body published in 1800. It is a scaled-down version of a life-size engraving displayed in the museum of the Department of Anatomy at the University of Edinburgh, and is based on a cadaver specially dissected and injected with mercury in 1788 by Alexander Monro secundus, probably with the assistance of Fyfe, who was his 'dissector'. Only recently has the relationship between these two engravings been established, and the Figure Legend in Fyfe's Compendium now provides the missing key to the features illustrated in the life-size engraving. The source and very variable quality of some of Fyfe's other plates, both in the Lymphatics section and elsewhere in the book, are discussed. PMID:9890727

  10. Enrico Fermi Awards Ceremony for Dr. Allen J. Bard and Dr. Andrew Sessler, February 2014 (Presentations, including remarks by Energy Secretary, Dr. Ernest Moniz)

    SciTech Connect

    Moniz, Ernest

    2014-02-03

    The Fermi Award is a Presidential award and is one of the oldest and most prestigious science and technology honors bestowed by the U.S. Government. On February 3, 2014 it was conferred upon two exceptional scientists. The first to be recognized is Dr. Allen J. Bard, 'for international leadership in electrochemical science and technology, for advances in photoelectrochemistry and photocatalytic materials, processes, and devices, and for discovery and development of electrochemical methods including electrogenerated chemiluminescence and scanning electrochemical microscopy.' The other honoree is Dr. Andrew Sessler, 'for advancing accelerators as powerful tools of scientific discovery, for visionary direction of the research enterprise focused on challenges in energy and the environment, and for championing outreach and freedom of scientific inquiry worldwide.' Dr. Patricia Dehmer opened the ceremony, and Dr. Ernest Moniz presented the awards.

  11. An Analysis and Comparison of Two Short Writings: "Inaugural Address at the University of St. Andrew's" by J.S. Mill and "The University of Utopia" by R.M. Hutchins, Based on Five Criteria.

    ERIC Educational Resources Information Center

    Poirier, Jeannine M.

    Focusing on the concept of education for work vs. education for living, the author presents a comparative analysis of two works on liberal education, each of which was originally delivered orally to university students: "The Inaugural Address at the University of St. Andrew" by John Stuart Mill and "The University of Utopia" by Robert Maynard…

  12. DFT analysis of g and 13C hyperfine coupling tensors for model Ni(I)(CO)(n)L(m) (n = 1-4, L = H2O, OH-) complexes epitomizing surface nickel(I) carbonyls.

    PubMed

    Pietrzyk, Piotr; Podolska, Katarzyna; Sojka, Zbigniew

    2008-11-27

    Relativistic calculations within the spin-orbit mean-field (SOMF) approximation, the zero-order regular approximation (ZORA), and the scalar relativistic method based on the Pauli Hamiltonian were performed for the prediction and interpretation of the electronic g tensor and (13)C hyperfine tensor for a set of model polycarbonyl nickel(I) complexes with aqua or hydroxy coligands. They exhibit extensive similarities with heterogeneous [Ni(I)(CO)(n)]-surface complexes produced upon adsorption of carbon monoxide on Ni(I) ions grafted on silica or inside the zeolite channels. Benchmark calculations showing the influence of the exchange-correlation functional on the g tensor were carried out for well-defined nickel(I) complexes of known structure. On this basis, the SOMF-B3LYP scheme was chosen for calculations of the g tensor, and the obtained results were in satisfactory agreement with literature EPR data found for the [Ni(I)(CO)(n)]/SiO(2) system. The calculated g and A((13)C) tensors allowed polycarbonyl complexes of various stereochemistries to be distinguished. The nature of the Deltag(ii) shifts was assessed in terms of the molecular orbital contributions due to the magnetic-field-induced couplings and their structure sensitivity. The noncoincidence of g and (13)C hyperfine principal axes and their orientation with respect to the molecular framework was also examined. The ability of DFT calculations to follow consistently variations of the EPR parameters induced by stereochemical changes around the Ni(I) center provides an invaluable reference for the interpretation of experimental results. PMID:18986126

  13. Comparisons of Aquatic Invertebrate Assemblages in Small, Old Growth and Second Growth Forested Catchments of the H.J. Andrews Experimental Forest, Oregon

    NASA Astrophysics Data System (ADS)

    Frady, C. H.; Johnson, S. L.; Li, J. L.

    2005-05-01

    In small streams, riparian vegetation often composes the food base for many aquatic invertebrates. Forest harvest can result in major changes to riparian vegetation. If abundance, richness, or community structure of invertebrate assemblages is affected by removal of riparian vegetation, are legacies of these practices evident 20-40 years post-harvest? If so, are these differences uniform through time, or are they temporally dependant? We investigated stream invertebrate assemblage dynamics between old growth and second growth forest types and across seasons in six small-basins in the H.J. Andrews Experimental Forest. Individual basins range from 500m to 1000m in elevation and 12.4ha to 98.1ha in area. Six benthic samples and four, 1-week emergence samples were collected in each basin each season (June 2003 through May 2004). Preliminary results from benthic samples suggest strong changes in community structure between seasons as additional taxa not found in summer (i.e. Agathon, Anagapetus, Chernokrilus, and Rhyacophila "angelita group") were collected in autumn and winter samples. However, results from summer comparisons demonstrate no differences in benthic and emergence abundance (p = 0.47, and p = 0.98, respectively) or benthic richness (p = 0.41) between old growth and second growth invertebrate assemblages.

  14. Flood of September 7-9, 1987, in Lexington and Richland counties in the vicinity of Saint Andrews Road and Irmo, South Carolina

    USGS Publications Warehouse

    Guimaraes, W.B.

    1989-01-01

    Localized heavy rainfall on September 7, 1987, in Lexington and Richland Counties, South Carolina, caused severe flooding in the basins of Kinley Creek, Rawls Creek, and Stoop Creek, in the vicinity of Saint Andrews Road and the town of Irmo, South Carolina. The flooding damaged homes, furnishings, and landscaping. Rainfall, peak discharges, high-water elevations, and frequency relations of rainfall and discharge are tabulated and plotted for selected streams. The rain was most intense in the area along Rawls Creek, R-2 (tributary to Rawls Creek), Koon Branch (tributary to Rawls Creek), and the upper part of Kinley Creek. A rainfall of about 5.5 inches in 3 hours, which has a recurrence interval in excess of 100 years, was reported by local residents along these streams. High-water marks are presented in this report for Stoop Creek, Kinley Creek, K-1 (tributary to Kinley Creek), K-2 (tributary to Kinley Creek), unnamed tributary to Kinley Creek, Lowery Creek (tributary to Kinley Creek), Rawls Creek, R-2 (tributary to Rawls Creek), and Koon Branch (tributary to Rawls Creek). Peak discharges at the most downstream sites on Rawls Creek and Koon Branch had recurrence intervals of 75 years and 60 years, respectively. Peak discharges on Kinley Creek varied from 20 to 25 years north of K-1 basin to less than 10 years at K-1. The Stoop Creek basin had a recurrence interval of 10 years. (USGS)

  15. Spatial distribution and compositional variation of APS minerals related to uranium deposits in the Kiggavik-Andrew Lake structural trend, Nunavut, Canada

    NASA Astrophysics Data System (ADS)

    Riegler, Thomas; Quirt, Dave; Beaufort, Daniel

    2016-02-01

    The Kiggavik-Andrew Lake structural trend consists of four mineralized zones, partially outcropping, lying 2 km south of the erosional contact with the unmetamorphosed sandstone and basal conglomerates of the Paleoproterozoic Thelon Formation. The mineralization is controlled by a major E-W fault system associated with illite and sudoite alteration halos developed in the Archean metagraywackes of the Woodburn Lake Group. Aluminum phosphate sulfate (APS) minerals from the alunite group crystallized in association with the clay minerals in the basement alteration halo as well as in the overlying sandstones, which underwent mostly diagenesis. APS minerals are Sr- and S-rich (svanbergite end-member) in the sedimentary cover overlying the unconformity, whereas they are light rare earth elements (LREE)-rich (florencite end-member) in the altered basement rocks below the unconformity. The geochemical signature of each group of APS minerals together with the petrography indicates three distinct generations of APS minerals related to the following: (1) paleoweathering of continental surfaces prior to the basin occurrence, (2) diagenetic processes during the burial history of the lower unit of the Thelon sandstones, and (3) hydrothermal alteration processes which accompanied the uranium deposition in the basement rocks and partially overlap the sedimentary-diagenetic mineral parageneses. In addition, the association of a first generation of APS minerals with both detrital cerium oxide and aluminum oxy-hydroxide highlights the fact that a part of the detrital material of the basal Thelon Formation originated from eroded paleolaterite (allochthonous regolith). The primary rare earth element (REE)-bearing minerals (e.g., monazite, REE carbonates, and allanite) of the host rocks were characterized to identify the potential sources of REE. The REE chemical composition highlights a local re-incorporation of the REE released from the alteration processes in the APS minerals of

  16. Ethanol extract of paeonia suffruticosa Andrews (PSE) induced AGS human gastric cancer cell apoptosis via fas-dependent apoptosis and MDM2-p53 pathways

    PubMed Central

    2012-01-01

    Background The root bark of Paeonia suffruticosa Andrews (PSE), also known as Moutan Cortex, has been widely used in Asia to treat various diseases. The molecular mechanisms by which PSE exerts its anti-oxidant and anti-inflammatory activities are well known, but its anti-cancer activity is not yet well understood. Here, we present evidence demonstrating that PSE can be used as a potent anti-cancer agent to treat gastric cancer. Methods The effects of the ethanol extract of PSE on cell proliferation were determined using an MTT (1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan) assay. Cell cytotoxicity induced by the PSE extact is measured using an LDH leakage assay. Flow cytometry was used to analyze the cell cycle and to measure the subG0/G1 apoptotic cell fraction. Apoptosis induced by the PSE extact is also examined using a DNA fragmentation assay. Western blot analysis is used to measure the levels of apoptotic proteins such as Fas receptor, caspase-8, caspase-3, PARP, Bax, Bcl-2, MDM2, and p53. Results This study demonstrated that treating AGS cells with the PSE extact significantly inhibited cell proliferation and induced cytotoxicity in a dose- and time-dependent manner. The PSE extract also induced apoptosis in AGS cells, as measured by flow cytometry and a DNA fragmentation assay. We found that the PSE extract induced apoptosis via the extrinsic Fas-mediated apoptosis pathway, which was concurrent with the activation of caspases, including caspase-8 and caspase-3, and cleavage of PARP. The MDM2-p53 pathway also played a role in the apoptosis of AGS cells that was induced by the PSE extract. Conclusions These results clearly demonstrate that the PSE extact displays growth-suppressive activity and induces apoptosis in AGS cells. Our data suggest that the PSE extact might be a potential anti-cancer agent for gastric cancer. PMID:22963678

  17. Computer Modeling of Hydrology, Weathering, and Isotopic Fractionation in Andrews Creek, Rocky Mountain National Park, Colorado for Water Years 1992 through 2012

    NASA Astrophysics Data System (ADS)

    Webb, R. M. T.; Parkhurst, D. L.; Mast, A.; Clow, D. W.

    2014-12-01

    The U.S. Geological Survey's (USGS) Water, Energy, and Biogeochemical Model (WEBMOD) was used to simulate hydrology, weathering, and isotopic fractionation in the 1.7 square kilometer Andrews Creek alpine watershed. WEBMOD includes hydrologic modules derived from the USGS Precipitation Runoff Modeling System, the National Weather Service Hydro-17 snow model, and TOPMODEL. PHREEQC, a geochemical reaction model, is coupled with the hydrologic model to simulate the geochemical evolution of waters as they evaporate, mix, and react within the landscape. Major solute concentrations and δ18O were modeled over the period 1992-2012. Variations of chloride and inorganic nitrogen respond almost entirely to variations in atmospheric deposition and preferential elution of snowpack. Both evaporation and melting result in isotopic enrichment of heavy isotopes in the residual snowpack throughout the summer. Magnesium and potassium, derived mostly from weathering with some atmospheric inputs, vary seasonally with uptake during the growing season and release during the fall and winter. The weathering of granitic minerals—oligoclase, biotite, chlorite, pyrite, calcite, and formation of secondary minerals—kaolinite, goethite, gibbsite, and smectite-illite—were selected as primary reactions based on mole-balance modeling of basin outflows. The rates of these reactions were quantified by calibrating WEBMOD to match observed concentrations and loads. Exported annual loads of most weathering products are highly correlated with discharge, whereas silica loads are less correlated with discharge, suggesting a source that is more active during dry years and less active during wet years. Potential sources include net dissolution of kaolinite and smectite-illite or mineralization of colloids with high silica content. WEBMOD is a valuable tool for simulating water quality variations in response to climate change, acid mine drainage, acid rain, biological transformations, and other

  18. Hyporheic Geophysics: D.C. Resistivity Imaging of Valley-bottom Alluvium in a 3rd-order Mountain Stream, HJ Andrews Experimental Forest, Oregon, USA

    NASA Astrophysics Data System (ADS)

    Zarnetske, J. P.; Haggerty, R.; Crook, N.; Robinson, D. A.

    2006-12-01

    The hyporheic zone (HZ) can serve as either a source or a sink for nutrients (e.g., nitrogen) and moderates biogeochemical and temperature signals in stream ecosystems. Understanding of the HZ is hampered by lack of subsurface images, particularly ones that are non-invasive and fast. Geophysical imaging, combined with extant empirical methods and hydrodynamic models provides an opportunity to improve precision in modeling HZ transport and nutrient retention. With the aid of CUAHSI HMF, we completed a d.c. resistivity survey of an existing stream denitrification study site (Mack Creek, H.J. Andrews Experimental Forest, OR, a Long-term Ecological Research site) to quantify the geometry of the valley bottom alluvial aquifer and HZ. The non- invasive d.c. resistivity survey successfully produced a quantitative 3-dimensional image of the variable alluvial aquifer thickness below and adjacent to the stream channel. We extensively imaged one area, 50 m x 28 m with 10 transects, in an old growth Douglas fir (Pseudotsuga menziesii) stand which incorporated a large woody debris log jam, to determine that alluvial thickness averages 4.1 m and varies between 0 m and 8 m. We completed a single 50 m longitudinal section in an adjacent downstream logged block (harvested 1964-65) to determine that alluvial thickness averages 0.3 m and varies between 0 m and 1 m. The presence of valley- bottom bedrock exposures at the study site helped to constrain and verify the d.c. resistivity interpretation of the bedrock-alluvium interface. Some d.c. survey challenges were encountered, including the confinement of electrode lines to the channel or near channel because dry organic layers or fallen trees across much of the forest floor prevented good electrode contact with the ground. Ultimately, this geophysics-enhanced knowledge of the stream-adjacent aquifer will allow for more accurate interpretation of hyporheic observations and parameterization of hyporheic hydraulic and denitrification

  19. H3K4me3 Stimulates the V(D)J RAG Complex for Both Nicking and Hairpinning in Trans in Addition to Tethering in Cis: Implications for Translocations

    PubMed Central

    Shimazaki, Noriko; Tsai, Albert G.; Lieber, Michael R.

    2010-01-01

    SUMMARY The PHD finger of the RAG2 polypeptide of the RAG1/RAG2 complex binds to the histone H3 modification, trimethylated lysine 4 (H3K4me3), and in some manner increases V(D)J recombination. However, in the absence of biochemical studies of H3K4me3 on purified RAG enzyme activity, the precise role of H3K4me3 has not been clear. Here, we find that H3K4me3 stimulates purified RAG enzymatic activity at both the nicking (2 to 5-fold) and hairpinning (3 to 11-fold) steps of V(D)J recombination. Remarkably, this stimulation can be achieved with free H3K4me3 peptide (in trans). This indicates that H3K4me3 functions via two distinct mechanisms. It not only tethers the RAG enzyme complex to a region of DNA, but it also induces a substantial increase in the catalytic turnover number (kcat) of the RAG complex. The H3K4me3 catalytic stimulation applies to suboptimal cryptic RSS sites located at H3K4me3 peaks that are critical in the inception of human T-cell acute lymphoblastic lymphomas. PMID:19524534

  20. Analysis of Apoptosis in Ultraviolet-Induced Sea Cucumber (Stichopus japonicus) Melting Using Terminal Deoxynucleotidyl-Transferase-Mediated dUTP Nick End-Labeling Assay and Cleaved Caspase-3 Immunohistochemistry.

    PubMed

    Yang, Jing-Feng; Gao, Rong-Chun; Wu, Hai-Tao; Li, Peng-Fei; Hu, Xian-Shu; Zhou, Da-Yong; Zhu, Bei-Wei; Su, Yi-Cheng

    2015-11-01

    The sea cucumber body wall melting phenomenon occurs under certain circumstances, and the mechanism of this phenomenon remains unclear. This study investigated the apoptosis in the ultraviolet (UV)-induced sea cucumber melting phenomenon. Fresh sea cucumbers (Stichopus japonicus) were exposed to UV radiation for half an hour at an intensity of 0.056 mW/cm(2) and then held at room temperature for melting development. The samples were histologically processed into formalin-fixed paraffin-embedded tissues. The apoptosis of samples was analyzed with the terminal deoxynucleotidyl-transferase-mediated dUTP nick end-labeling (TUNEL) assay and cleaved caspase-3 immunohistochemistry. The emergence of TUNEL-positive cells speeds up between 0.5 and 2 h after UV irradiation. Cleaved caspase-3 positive cells were obviously detected in sample tissues immediately after the UV irradiation. These results demonstrated that sea cucumber melting induced by UV irradiation was triggered by the activation of caspase-3 followed by DNA fragmentation in sea cucumber tissue, which was attributed to apoptosis but was not a consequence of autolysis activity. PMID:26484758

  1. Deep versus shallow melt stagnation in an ultra-slow / ultra-cold ridge segment: the Andrew Bain southern RTI (SWIR)

    NASA Astrophysics Data System (ADS)

    Paganelli, E.; Brunelli, D.; Seyler, M.; Bonatti, E.; Cipriani, A.; Ligi, M.

    2011-12-01

    The Andrew Bain Fracture Zone (ABFZ) represents one of the largest transform faults in the ridge system spanning 750 km in length with a characteristic lens-shape structure. The southern Ridge-Transform Intersection represents the deepest sector of the whole South West Indian Ridge system. During the Italian-Russian expedition S23-AB06, the seafloor in the Southern Ridge Transform Intersection (RTI) has been sampled recovering only ultramafic material in the majority of the dredging sites. The sampled spinel and plagioclase peridotites show hybrid textures, characterized either by deep spinel-field impregnation assemblages (sp+cpx±opx±ol) or by plagioclase-field equilibrated patches and mineral trails (pl+cpx±ol) marked by both crystallization of newly formed plagioclase-field equilibrated trails and formation of plagioclase coronas around spinel. The ones collected from ridge axis show also late gabbroic pockets and veins, variably enriched in clinopyroxene. Overall textures account for important melt percolation/stagnation events occurred in the plagioclase and spinel field. Major and trace element distribution in pyroxenes and spinels from spinel-bearing peridotites overall follow a general melting trend accompanied by a progressive re-equilibration to lower P/T facies at all scales. However, only few samples can be linked to near fractional melting, while the majority of them shows REE pattern and trace element concentrations that cannot be reproduced by fractional melting process. Open-system melting (OSM) better reproduces measured REE patterns. Modeling melting in an open system scenario requires high residual porosity to be accounted for along with generally enriched melts to influx the melting parcel at depth. Melting at high residual porosity suggests a near-batch regime in which enriched melts stagnate in the spinel field. Inhibition of melt segregation during melt/rock interaction asks for a permeability barrier to develop in the region where the

  2. Spatial and Temporal Trends in the Carbon, Nitrogen, and Sulfur Isotopes of Stream DOM From 10 Watersheds at the HJ Andrews Experimental Forest.

    NASA Astrophysics Data System (ADS)

    Frentress, J.; Kendall, C.; Lajtha, K.; Jones, J.

    2008-12-01

    In order to better understand sources of dissolved organic matter (DOM) in streams from the small to large watershed scales, we initiated a one-year investigation of the chemical and isotopic characteristics of DOM at the HJ Andrews Experimental Forest (HJA) in Blue River, OR. DOM is a biologically significant loss from these watersheds, but its sources (forest floor, mineral soil, riparian zones, stream biota) are debated. Traditional chemical characterizations of DOM like SUVA and FI have been useful in conceptualizing and modeling streamflow sources, however, an improved method for assessing DOM quality is needed to adequately differentiate DOM from sources within the watershed. The isotopic characterization of inorganic molecules like nitrate has provided insight to the role of subsurface and surface processes governing the production and transport of critical nutrients, and yet to date, little work has been done to examine the usefulness of isotopic characterization of organically bound nutrients. We apply the isotopic characterization approach to DOM in order to better understand DOM production, transformation, and transport to streams in a range of watershed sizes. Major questions addressed in this research are: 1) Where in the watershed does stream DOM come from? 2) How do DOM sources vary temporally? 3) How do physical attributes of the watershed mediate DOM quality? A relatively new solid-phase extraction technique using C-18 resin was used to isolate DOM in water samples from 10 watersheds, ranging in size from 10 to 6200 hectares, on 3-week intervals from May 2007 to June 2008. The modified technique allowed for small (1 Liter) sample sizes and short processing times to reduce the costs of analysis. The capacity of carbon, nitrogen and sulfur isotopic characterizations of DOM, as well as traditional methods like SUVA and C:N, to predict physical watershed attributes (i.e. mean residence time, soil depth, elevation, gradient) and land use history (timber

  3. Target-triggered three-way junction structure and polymerase/nicking enzyme synergetic isothermal quadratic DNA machine for highly specific, one-step, and rapid microRNA detection at attomolar level.

    PubMed

    Zhang, Qing; Chen, Feng; Xu, Feng; Zhao, Yongxi; Fan, Chunhai

    2014-08-19

    MicroRNAs (miRNAs) play important roles in many biological processes and are regarded as promising cancer biomarkers. Herein, a highly specific, one-step, and rapid miRNAs detection strategy with attomolar sensitivity has been developed on the basis of a target-triggered three-way junction (3-WJ) structure and polymerase/nicking enzyme synergetic isothermal quadratic DNA machine (ESQM). To this end, 3-WJ probes (primer and template) are designed to selectively recognize target miRNA and form the stable 3-WJ structure to trigger ESQM, resulting in a high quadratic amplified signal. A high specificity is demonstrated by the excellent discrimination of even single-base mismatched homologous sequences with mismatched bases in varied locations (close to the 3'-end, the 5'-end, and the middle). In addition, a low detection limit down to 2 amol was achieved within 30 min. This sensitivity is much higher than those of most linear amplification-based approaches and is even comparable to those of some exponential amplification-based methods. Furthermore, the applicability of this method in complex samples was demonstrated by the analysis of cancer cell small RNA extracts, results of which were in good agreement with those obtained by a commercial miRNA kit and previously published data. The miRNA with a 3' end modification (2'-O-methylation), such as plant miRNA, was also successfully detected, confirming the good universality of the proposed strategy. It is worthwhile to point out that several well-established methods using miRNA as primer for polymerization reaction are of relatively poor performance in the analysis of these modified miRNA. Therefore, these merits endow the developed strategy with powerful implications for biological research and an effective diagnostic assay. PMID:25072308

  4. Magnitude and extent of sediment toxicity in four bays of the Florida Panhandle: Pensacola, Choctawhatchee, St. Andrew and Apalachicola. National status and trends program for marine environmental quality: Technical memo

    SciTech Connect

    Long, E.R.; Sloane, G.M.; Carr, R.S.; Scott, K.J.; Thursby, G.B.

    1997-10-01

    The toxicity of sediments in Pensacola, Choctawhatcheee, St. Andrew and Apalachicola Bays was determined as part of bioeffects assessments performed by NOAA`s National Status and Trends Program. The objectives of the survey were to determine: (1) the spatial patterns in toxicity throughout each bay, (2) the spatial extent of toxicity throughout and among the bays, (3) the severity of degree of toxicity, and (4) the relationships between chemical contamination and toxicity. The survey was conducted over two years: Pensacola Bay and St. Andrew Bay were sampled in 1993; and Choctawhatchee Bay, Apalachicola Bay and Bayou Chico (a sub-basin of Pensacola Bay) were sampled during 1994. Surficial sediment samples were collected from 123 randomly-chosen locations throughout the five areas. Multiple toxicity tests were conducted on all samples, and chemical analyses were performed on 102 of the 123 samples. Toxicological tests were conducted to determine survival, reproductive success, morphological development, metabolic activity, and genotoxicity; all bays showed toxicity in at least some of the samples.

  5. A comparison of mercury burdens between St. Marks National Wildlife Refuge and St. Andrew Bay, Florida: Evaluation of fish body burdens and physiological responses in largemouth bass, spotted seatrout, striped mullet, and sunfish

    USGS Publications Warehouse

    Huge, D.H.; Rauschenberger, R.H.; Wieser, F.M.; Hemming, J.M.

    2011-01-01

    Musculature from the dorsal region of 130 largemouth bass (Micropterus salmoides), 140 sunfish (Lepomis sp.), 41 spotted seatrout (Cynoscion nebulosus) and 67 striped mullet (Mugil cephalus) were collected from five estuarine and five freshwater sites within the St. Marks National Wildlife Refuge and two estuarine and two freshwater sites from St. Andrew Bay, Florida, United States of America. Musculature was analyzed for total mercury content, sagittal otoliths were removed for age determination and physiological responses were measured. Largemouth bass and sunfish from the refuge had higher mercury concentrations in musculature than those from the bay. Male spotted seatrout, male striped mullet, male and female sunfish and female largemouth bass had mercury burdens positively correlated with length. The majority of all four species of fish from both study areas contained mercury levels below 1.5 part per million, the limit for safe consumption recommended the Florida Department of Health. In comparison, a significant percentage of largemouth bass and sunfish from several sampled sites, most notably Otter Lake and Lake Renfroe within St. Marks National Wildlife Refuge, had mercury levels consistent with the health department's guidelines of 'limited consumption' or 'no consumption guidelines.'

  6. Wireless Andrew: Everywhere You Want To Be.

    ERIC Educational Resources Information Center

    Futhey, Tracy

    2000-01-01

    Describes the wireless local area network at Carnegie Mellon University. Highlights include classroom applications, particularly in the Business School; the use of laptop computers configured with wireless technology; handheld computers, including use for testing; and assuring appropriate uses of wireless technology. (LRW)

  7. The Future of the Andrew File System

    ScienceCinema

    None

    2011-04-25

    The talk will discuss the ten operational capabilities that have made AFS unique in the distributed file system space and how these capabilities are being expanded upon to meet the needs of the 21st century. Derrick Brashear and Jeffrey Altman will present a technical road map of new features and technical innovations that are under development by the OpenAFS community and Your File System, Inc. funded by a U.S. Department of Energy Small Business Innovative Research grant. The talk will end with a comparison of AFS to its modern days competitors.

  8. The Future of the Andrew File System

    SciTech Connect

    2011-02-23

    The talk will discuss the ten operational capabilities that have made AFS unique in the distributed file system space and how these capabilities are being expanded upon to meet the needs of the 21st century. Derrick Brashear and Jeffrey Altman will present a technical road map of new features and technical innovations that are under development by the OpenAFS community and Your File System, Inc. funded by a U.S. Department of Energy Small Business Innovative Research grant. The talk will end with a comparison of AFS to its modern days competitors.

  9. Nick Sagan Reflects on Voyager 1 and the Golden Record

    NASA Astrophysics Data System (ADS)

    Showstack, Randy

    2013-10-01

    When scientists confirmed on 12 September that NASA's Voyager 1 spacecraft had entered interstellar space (Eos, 94(39), 339, doi:10.1002/2013EO390003), the probe was acknowledged as the first human-made object to travel into that realm. The probe and its twin, Voyager 2, each carry a 12-inch gold-plated copper disk, known as the Golden Record.

  10. A resolution honoring the life of Andrew Wyeth.

    THOMAS, 111th Congress

    Sen. Casey, Robert P., Jr. [D-PA

    2009-01-27

    01/27/2009 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S946-947; text as passed Senate: CR S947; text of measure as introduced: CR S899-900) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  11. Speaking Unassisted: Comments on a Paper by Andrews et al.

    ERIC Educational Resources Information Center

    Wingate, Marcel E.

    1983-01-01

    In a reply to a review of replicated findings on stuttering, the author adds 14 points concerning symptoms, prevalence, incidence, stutterer-nonstutterer differences, and variability of stuttering. He takes exception to the review's statements on treatment and theories of stuttering. (CL)

  12. General Christopher C. Andrews: Leading the Minnesota Forestry Revolution

    ERIC Educational Resources Information Center

    Rice, Anna M.

    2002-01-01

    In the nineteenth century, America's burgeoning population certainly did grab all the timber it could. Vast pine forests stretched from Maine to Dakota, and the lumber industry voraciously consumed them from east to west. In 1800, the Minnesota territory was sparsely sprinkled with fur traders and American Indians. By 1850, its bounteous forests…

  13. Investigation by EPR and ENDOR spectroscopy of the nickel(I) form of cofactor F{sub 430} of Methanobacterium thermoautorophicum and of nickel(I) octaethylisobacteriochlorin

    SciTech Connect

    Telser, J.; Fann, Y.C.; Hoffman, B.M.; Renner, M.W.; Fajer, J.; Wang, S.; Zhang, H.; Scott, R.A.

    1997-01-29

    The terminal step in methane generation by the archaeon Methanobacterium thermoautotrophicum is catalyzed by the enzyme S-methyl coenzyme M reductase (methylreductase). This enzyme contains a Ni(II) tetrapyrrole cofactor, F{sub 430}, at the active site in the resting state. A Ni(I) state (Ni{sup I}F{sub 430}) has been proposed as the active form of the cofactor. Nickel isobacteriochlorins have been used to model F{sub 430}. We have investigated both Ni{sup I}F{sub 430} and Ni{sup I}OEiBC using CW and pulsed EPR and ENDOR spectroscopy at X-band and Q-band microwave frequencies. In agreement with a previous X-band EPR and ESEEM study, at Q-band, the g tensor of Ni{sup I}F{sub 430} appears axial and {sup 1,2}H ENDOR of Ni{sup I}F{sub 430} in H{sub 2}O versus D{sub 2}O solvent shows no evidence for strongly coupled, solvent-exchangeable hydrogens, and this indicates that there is no water axially coordinated to Ni(I) in contrast to the Ni(II) resting state. Both Ni{sup I}F{sub 430} and Ni{sup I}OEiBC give {sup 14}N ENDOR signals arising from the four pyrrole nitrogen ligands to Ni(I). Previous EXAFS studies of Ni{sup I}OEiBC and Ni{sup I}F{sub 430}Me{sub 5} showed two sets of Ni(I)-N distances differing by nearly 8%, and in agreement with this, the {sup 14}N ENDOR signals for both Ni{sup I}OEiBC and Ni{sup I}F{sub 430} can be analyzed in terms of superimposed signals from two distinct types of nitrogen ligand. 86 refs., 5 figs., 3 tabs.

  14. Magnetic Contribution to Heat Capacity and Entropy of Nicke Ferrite (NiFe2O4)

    SciTech Connect

    S Ziemniak, L Anovitz, R Castelli

    2005-12-15

    The heat capacity of nickel ferrite was measured as a function of temperature over the range from 50 to 1200 C using a differential scanning calorimeter. A thermal anomaly was observed at 584.9 C, the expected Curie temperature, T{sub c}. The observed behavior was interpreted by recognizing the sum of three contributions: (1) lattice (vibrational), (2) a spin wave (magnetic) component and (3) a {lambda}-transition (antiferromagnetic-paramagnetic transition) at the Curie temperature. The first was modeled using vibrational frequencies derived from an experimentally-based ir absorption spectrum, while the second was modeled using a spin wave analysis that provided a T{sup 3/2} dependency in the low temperature limit, but incorporated an exchange interaction between cation spins in the octahedral and tetrahedral sites at elevated temperatures, as first suggested by Grimes [15]. The {lambda}-transition was fitted to an Inden-type model which consisted of two truncated power law series in dimensionless temperature (T/T{sub c}). Exponential equality was observed below and above T{sub c}, indicating symmetry about the Curie temperature. Application of the methodology to existing heat capacity data for other transition metal ferrites (AFe{sub 2}O{sub 4}, A = Fe, Co) revealed the same exponential equality, i.e., m = n = 5.

  15. Processing of Nonconjugative Resistance Plasmids by Conjugation Nicking Enzyme of Staphylococci

    PubMed Central

    Pollet, Rebecca M.; Ingle, James D.; Hymes, Jeff P.; Eakes, Thomas C.; Eto, Karina Yui; Kwong, Stephen M.; Ramsay, Joshua P.; Firth, Neville

    2016-01-01

    ABSTRACT Antimicrobial resistance in Staphylococcus aureus presents an increasing threat to human health. This resistance is often encoded on mobile plasmids, such as pSK41; however, the mechanism of transfer of these plasmids is not well understood. In this study, we first examine key protein-DNA interactions formed by the relaxase enzyme, NES, which initiates and terminates the transfer of the multidrug resistance plasmid pSK41. Two loops on the NES protein, hairpin loops 1 and 2, form extensive contacts with the DNA hairpin formed at the oriT region of pSK41, and here we establish that these contacts are essential for proper DNA cleavage and religation by the full 665-residue NES protein in vitro. Second, pSK156 and pCA347 are nonconjugative Staphylococcus aureus plasmids that contain sequences similar to the oriT region of pSK41 but differ in the sequence predicted to form a DNA hairpin. We show that pSK41-encoded NES is able to bind, cleave, and religate the oriT sequences of these nonconjugative plasmids in vitro. Although pSK41 could mobilize a coresident plasmid harboring its cognate oriT, it was unable to mobilize plasmids containing the pSK156 and pCA347 variant oriT mimics, suggesting that an accessory protein like that previously shown to confer specificity in the pWBG749 system may also be involved in transmission of plasmids containing a pSK41-like oriT. These data indicate that the conjugative relaxase in trans mechanism recently described for the pWBG749 family of plasmids also applies to the pSK41 family of plasmids, further heightening the potential significance of this mechanism in the horizontal transfer of staphylococcal plasmids. IMPORTANCE Understanding the mechanism of antimicrobial resistance transfer in bacteria such as Staphylococcus aureus is an important step toward potentially slowing the spread of antimicrobial-resistant infections. This work establishes protein-DNA interactions essential for the transfer of the Staphylococcus aureus multiresistance plasmid pSK41 by its relaxase, NES. This enzyme also processed variant oriT-like sequences found on numerous plasmids previously considered nontransmissible, suggesting that in conjunction with an uncharacterized accessory protein, these plasmids may be transferred horizontally via a relaxase in trans mechanism. These findings have important implications for our understanding of staphylococcal resistance plasmid evolution. PMID:26728193

  16. Hematopoietic Stem Cell Transplantation for Primary Immunodeficiencies By Elizabeth Kang and Andrew Gennery

    PubMed Central

    Kang, Elizabeth; Gennery, Andrew

    2014-01-01

    Allogeneic Hematopoietic Stem Cell Transplantation has been shown to be curative for well described as well as newly discovered immunodeficiencies. However it is difficulty to define a universal transplant regimen given the rarity of these disorders and the varied pathophysiology these disorders encompass. This review will discuss those primary immunodeficiencies most commonly treated by hematopoietic stem cell transplant and describe the transplant issues specific to these disorders. PMID:25459185

  17. Multiple species of wild tree peonies gave rise to the 'king of flowers', Paeonia suffruticosa Andrews.

    PubMed

    Zhou, Shi-Liang; Zou, Xin-Hui; Zhou, Zhi-Qin; Liu, Jing; Xu, Chao; Yu, Jing; Wang, Qiang; Zhang, Da-Ming; Wang, Xiao-Quan; Ge, Song; Sang, Tao; Pan, Kai-Yu; Hong, De-Yuan

    2014-12-22

    The origin of cultivated tree peonies, known as the 'king of flowers' in China for more than 1000 years, has attracted considerable interest, but remained unsolved. Here, we conducted phylogenetic analyses of explicitly sampled traditional cultivars of tree peonies and all wild species from the shrubby section Moutan of the genus Paeonia based on sequences of 14 fast-evolved chloroplast regions and 25 presumably single-copy nuclear markers identified from RNA-seq data. The phylogeny of the wild species inferred from the nuclear markers was fully resolved and largely congruent with morphology and classification. The incongruence between the nuclear and chloroplast trees suggested that there had been gene flow between the wild species. The comparison of nuclear and chloroplast phylogenies including cultivars showed that the cultivated tree peonies originated from homoploid hybridization among five wild species. Since the origin, thousands of cultivated varieties have spread worldwide, whereas four parental species are currently endangered or on the verge of extinction. The documentation of extensive homoploid hybridization involved in tree peony domestication provides new insights into the mechanisms underlying the origins of garden ornamentals and the way of preserving natural genetic resources through domestication. PMID:25377453

  18. 77 FR 59163 - Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-09-26

    ... CONTACT: Victor Wyant, 864-638-9568. Correction 1. In the Federal Register of March 8, 2010, in FR/Vol. 75... expected by December 2012. 2. In the Federal Register of March 8, 2010, in FR/Vol. 75, No. 44, on page..., 2010, in FR/Vol. 75, No. 44, on page 10458, in the first column, correct the ``Purpose and Need...

  19. Involvement of Colonizing Bacillus Isolates in Glucovanillin Hydrolysis during the Curing of Vanilla planifolia Andrews

    PubMed Central

    Chen, Yonggan; Li, Jihua; He, Shuzhen; Xu, Fei; Fang, Yiming

    2015-01-01

    Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-d-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-d-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-d-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation. PMID:25979899

  20. Detectives Nemorin and Andrews Anti-Gun Trafficking Act of 2013

    THOMAS, 113th Congress

    Rep. King, Peter T. [R-NY-2

    2013-02-14

    04/08/2013 Referred to the Subcommittee on Crime, Terrorism, Homeland Security, And Investigations. (All Actions) Tracker: This bill has the status IntroducedHere are the steps for Status of Legislation:

  1. Enhancing Pupils' Aspirations to University: The St Andrews Sutton Trust School Experience

    ERIC Educational Resources Information Center

    Lasselle, Laurence; Keir, Fraser; Smith, Ian

    2009-01-01

    The Sutton Trust Summer School offers to S5/Year 12 pupils the opportunity to sample student life for a week at one of five selecting universities in the UK. Most of the participants on the Sutton Trust Summer School will be the first generation in their family to attend university and have come from schools which traditionally send low numbers to…

  2. A resolution honoring former Senator and Rear Admiral Jeremiah Andrew Denton, Jr.

    THOMAS, 113th Congress

    Sen. Sessions, Jeff [R-AL

    2014-03-31

    03/31/2014 Submitted in the Senate, considered, and agreed to without amendment and with a preamble by Unanimous Consent. (consideration: CR S1881; text as passed Senate: CR S1874) (All Actions) Tracker: This bill has the status Passed SenateHere are the steps for Status of Legislation:

  3. Conditions for Learning. Paul Andrews Compares Schools in Five European Countries

    ERIC Educational Resources Information Center

    Andrews, Paul

    2006-01-01

    Recently, a multinational team of researchers on a European Union-funded project examined the teaching of mathematics in the age range 10-14 in England, Flemish Belgium, Finland, Hungary, and Spain. In this article, the author (who was a member of this team) discusses how the physical and social characteristics of schools create the conditions for…

  4. Seizing Opportunities: Genie Tyburski--Ballard Spahr Andrews & Ingersoll, LLP, Philadelphia

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    Genie Tyburski did not set out to be a law librarian. When asked at Drexel's library school what kind of librarian she wanted to be, she was surprised that "a good one" was not one of the options. But six weeks into the semester, she landed a part-time cataloging job at Community Legal Services in Philadelphia; six months later she was the library…

  5. 75 FR 10457 - Andrew Pickens Ranger District; South Carolina; AP Loblolly Pine Removal and Restoration Project

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-03-08

    ... manual and mechanical treatment. Woodlands are forests with relatively low tree densities of 25-60... density of trees in these stands range from sparse to a dense stocking basal area of 160 square feet per... as add to habitat diversity. Woodlands are forests with relatively low tree densities of...

  6. 76 FR 57043 - Andrew N. Finkel; Analysis of Proposed Consent Order to Aid Public Comment

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-15

    ... service. Visit the Commission Web site at http://www.ftc.gov to read this Notice and the news release... Pwner which respondent developed and sold in Google's Android Marketplace. Respondent claimed that...

  7. Detecting Outliers in Marathon Data by Means of the Andrews Plot

    NASA Astrophysics Data System (ADS)

    Stehlík, Milan; Wald, Helmut; Bielik, Viktor; Petrovič, Juraj

    2011-09-01

    For an optimal race performance, it is important, that the runner keeps steady pace during most of the time of the competition. First time runners or athletes without many competitions often experience an "blow out" after a few kilometers of the race. This could happen, because of strong emotional experiences or low control of running intensity. Competition pace of half marathon of the middle level recreational athletes is approximately 10 sec quicker than their training pace. If an athlete runs the first third of race (7 km) at a pace that is 20 sec quicker than is his capacity (trainability), he would experience an "blow out" in the last third of the race. This would be reflected by reducing the running intensity and inability to keep steady pace in the last kilometers of the race and in the final time as well. In sports science, there are many diagnostic methods ([3], [2], [6]) that are used for prediction of optimal race pace tempo and final time. Otherwise there is lacking practical evidence of diagnostics methods and its use in the field (competition, race). One of the conditions that needs to be carried out is that athletes have not only similar final times, but it is important that they keep constant pace as much as possible during whole race. For this reason it is very important to find outliers. Our experimental group consisted of 20 recreational trained athletes (mean age 32,6 years±8,9). Before the race the athletes were instructed to run on the basis of their subjective feeling and previous experience. The data (running pace of each kilometer, average and maximal heart rate of each kilometer) were collected by GPS-enabled personal trainer Forerunner 305.

  8. Involvement of Colonizing Bacillus Isolates in Glucovanillin Hydrolysis during the Curing of Vanilla planifolia Andrews.

    PubMed

    Chen, Yonggan; Gu, Fenglin; Li, Jihua; He, Shuzhen; Xu, Fei; Fang, Yiming

    2015-08-01

    Vanilla beans were analyzed using biochemical methods, which revealed that glucovanillin disperses from the inner part to the outer part of the vanilla bean during the curing process and is simultaneously hydrolyzed by β-d-glucosidase. Enzymatic hydrolysis was found to occur on the surface of the vanilla beans. Transcripts of the β-d-glucosidase gene (bgl) of colonizing microorganisms were detected. The results directly indicate that colonizing microorganisms are involved in glucovanillin hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences showed that the colonizing microorganisms mainly belonged to the Bacillus genus. bgl was detected in all the isolates and presented clustering similar to that of the isolate taxonomy. Furthermore, inoculation of green fluorescent protein-tagged isolates showed that the Bacillus isolates can colonize vanilla beans. Glucovanillin was metabolized as the sole source of carbon in a culture of the isolates within 24 h. These isolates presented unique glucovanillin degradation capabilities. Vanillin was the major volatile compound in the culture. Other compounds, such as α-cubebene, β-pinene, and guaiacol, were detected in some isolate cultures. Colonizing Bacillus isolates were found to hydrolyze glucovanillin in culture, indirectly demonstrating the involvement of colonizing Bacillus isolates in glucovanillin hydrolysis during the vanilla curing process. Based on these results, we conclude that colonizing Bacillus isolates produce β-d-glucosidase, which mediates glucovanillin hydrolysis and influences flavor formation. PMID:25979899

  9. 75 FR 32221 - Whirlpool Corporation, Evansville Division, Including On-Site Leased Workers from Andrews...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-06-07

    ... (75 FR 10321). At the request of the State Agency, the Department reviewed the certification for... under Chapter 2 of Title II of the Trade Act of 1974, as amended. Elliott S. Kushner, Certifying...

  10. Structures of minute virus of mice replication initiator protein N-terminal domain: Insights into DNA nicking and origin binding

    SciTech Connect

    Tewary, Sunil K.; Liang, Lingfei; Lin, Zihan; Lynn, Annie; Cotmore, Susan F.; Tattersall, Peter; Zhao, Haiyan; Tang, Liang

    2015-02-15

    Members of the Parvoviridae family all encode a non-structural protein 1 (NS1) that directs replication of single-stranded viral DNA, packages viral DNA into capsid, and serves as a potent transcriptional activator. Here we report the X-ray structure of the minute virus of mice (MVM) NS1 N-terminal domain at 1.45 Å resolution, showing that sites for dsDNA binding, ssDNA binding and cleavage, nuclear localization, and other functions are integrated on a canonical fold of the histidine-hydrophobic-histidine superfamily of nucleases, including elements specific for this Protoparvovirus but distinct from its Bocaparvovirus or Dependoparvovirus orthologs. High resolution structural analysis reveals a nickase active site with an architecture that allows highly versatile metal ligand binding. The structures support a unified mechanism of replication origin recognition for homotelomeric and heterotelomeric parvoviruses, mediated by a basic-residue-rich hairpin and an adjacent helix in the initiator proteins and by tandem tetranucleotide motifs in the replication origins. - Highlights: • The structure of a parvovirus replication initiator protein has been determined; • The structure sheds light on mechanisms of ssDNA binding and cleavage; • The nickase active site is preconfigured for versatile metal ligand binding; • The binding site for the double-stranded replication origin DNA is identified; • A single domain integrates multiple functions in virus replication.

  11. Talking about Life

    NASA Astrophysics Data System (ADS)

    Impey, Chris

    2010-08-01

    Preface; Part I. Introduction Timothy Ferris, Iris Fry, Steven Dick, Ann Druyan, Pinky Nelson, Neil Tyson, Steve Benner and William Bains; Part II. Earth Roger Buick, Lynn Rothschild, John Baross, Joe Kirschvink, Andrew Knoll, Simon Conway Morris, Roger Hanlon and Lori Marino; Part III. Solar System Chris McKay, David Grinspoon, Jonathan Lunine, Carolyn Porco, Laurie Leshin, Guy Consolmagno and Peter Smith; Part IV. Exoplanets Alan Boss, Geoff Marcy, Debra Fischer, Sara Seager, David Charbonneau and Vikki Meadows; Part V. Frontiers Jill Tarter, Seth Shostak, Ray Kurzweil, Nick Bostrom, Paul Davies, Martin Rees, Ben Bova and Jennifer Michael Hecht; Reading list; Glossary; Index.

  12. Relationship of Ordovician and Silurian reservoir development to unconformities at Midland farms and Inez fields, Andrews County, Texas

    SciTech Connect

    Mear, C.E.; Becher, J.W.

    1986-03-01

    Hydrocarbons are being produced at Midland Farms and Inez fields from Ellenburger dolomites and Fusselman limestones. Reservoirs developed there during Ordovician and Silurian periods of minor folding and faulting, followed by regional uplift and subaerial exposure of the carbonates. Vuggy, cavernous, and solution-enlarged fracture porosity was developed in the Lower Ordovician Ellenburger dolomites prior to deposition of the overlying Middle Ordovician shales of the Simpson Group. Vuggy and cavernous porosity developed in the Lower Silurian Fusselman crinoid-ostracod-pellet packstones and grainstones before deposition of the overlying Silurian Wristen shales. Montoya siliceous limestones of Late Ordovician age were truncated during a period of pre-Silurian erosion, but porosity development is not indicated in Montoya rock cuttings. Only minor amounts of porosity developed in the Lower to Middle Devonian Thirty-one packstones and wackestones as a result of uplift and erosion in the Middle Devonian. Regional compression during the post-Mississippian enhanced doubly plunging anticlines now having up to 91 m (300 ft) of closure at the Ellenburger through Thirty-one formations at Midland Farms and Inez fields. Fractures may have developed in Paleozoic limestones during this period of folding, but reservoir enhancement appears to have resulted only in the Ellenburger dolomites. Representative porosity measurements of the Ellenburger and Fusselman pay zones cannot be made from wireline log calculations, due to the fractured, vuggy, and cavernous nature of the porosity.

  13. 78 FR 21921 - Andrew Peklo III; Notice of Application Accepted for Filing, Intent To Waive Scoping, Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-12

    ... copy of the water quality certification; (2) a copy of the request for certification, including proof... refers to the docket for the application for license (P- 12790-002). All stakeholder comments, motions to intervene, and protests filed on the proceeding for the application for exemption from licensing...

  14. 76 FR 12094 - Andrew Peklo III; Notice of Application Tendered for Filing With the Commission and Soliciting...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-04

    ....net . i. FERC Contact: Steve Kartalia, (202) 502-6131 or Stephen.kartalia@ferc.gov . j. Cooperating....gov/docs-filing/ferconline.asp). Commenters can submit brief comments up to 6,000 characters, without prior registration, using the eComment system at http://www.ferc.gov/docs-filing/ecomment.asp . You...

  15. "Show Me Where You Study!"--An Interactive Project between German Language Students in Nottingham and St Andrews

    ERIC Educational Resources Information Center

    Hartung, Insa; Reisenleutner, Sandra

    2016-01-01

    Interactive projects among students of a Common European Framework of Reference for languages (CEFR) A1+/A2 level seem difficult to set up due to the limited language repertoire of the students. Thus, our aim was to take up the challenge and start a project with the objective of applying their language skills. We chose a collaborative approach to…

  16. Multiple species of wild tree peonies gave rise to the ‘king of flowers’, Paeonia suffruticosa Andrews

    PubMed Central

    Zhou, Shi-Liang; Zou, Xin-Hui; Zhou, Zhi-Qin; Liu, Jing; Xu, Chao; Yu, Jing; Wang, Qiang; Zhang, Da-Ming; Wang, Xiao-Quan; Ge, Song; Sang, Tao; Pan, Kai-Yu; Hong, De-Yuan

    2014-01-01

    The origin of cultivated tree peonies, known as the ‘king of flowers' in China for more than 1000 years, has attracted considerable interest, but remained unsolved. Here, we conducted phylogenetic analyses of explicitly sampled traditional cultivars of tree peonies and all wild species from the shrubby section Moutan of the genus Paeonia based on sequences of 14 fast-evolved chloroplast regions and 25 presumably single-copy nuclear markers identified from RNA-seq data. The phylogeny of the wild species inferred from the nuclear markers was fully resolved and largely congruent with morphology and classification. The incongruence between the nuclear and chloroplast trees suggested that there had been gene flow between the wild species. The comparison of nuclear and chloroplast phylogenies including cultivars showed that the cultivated tree peonies originated from homoploid hybridization among five wild species. Since the origin, thousands of cultivated varieties have spread worldwide, whereas four parental species are currently endangered or on the verge of extinction. The documentation of extensive homoploid hybridization involved in tree peony domestication provides new insights into the mechanisms underlying the origins of garden ornamentals and the way of preserving natural genetic resources through domestication. PMID:25377453

  17. Closure to "Modeling the Evolution of Incised Streams. II: Streambank Erosion" by Eddy J. Langendoen and Andrew Simon

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In 2008 Langendoen and Simon presented a numerical model in the Journal of Hydraulic Engineering (JHE) that predicts streambank erosion within the one-dimensional channel evolution computer model CONCEPTS. The model was validated against observed retreat of the outer bank of the Goodwin Creek Bendwa...

  18. 78 FR 27126 - East Bay, St. Andrews Bay and the Gulf of Mexico at Tyndall Air Force Base, Florida; Restricted...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-05-09

    ... WGS84 datum for its imagery base and imagery dated January 1, 2012. (2) The areas described in... at Tyndall Air Force Base, Florida; Restricted Areas AGENCY: U.S. Army Corps of Engineers, DoD... establishing six new restricted areas along the Tyndall Air Force Base (AFB) facility shoreline. Tyndall AFB...

  19. Assessing the skill of hydrology models at simulating the water cycle in the HJ Andrews LTER: Assumptions, strengths and weaknesses

    EPA Science Inventory

    Simulated impacts of climate on hydrology can vary greatly as a function of the scale of the input data, model assumptions, and model structure. Four models are commonly used to simulate streamflow in model assumptions, and model structure. Four models are commonly used to simu...

  20. Comparison of four kinds of extraction techniques and kinetics of microwave-assisted extraction of vanillin from Vanilla planifolia Andrews.

    PubMed

    Dong, Zhizhe; Gu, Fenglin; Xu, Fei; Wang, Qinghuang

    2014-04-15

    Vanillin yield, microscopic structure, antioxidant activity and overall odour of vanilla extracts obtained by different treatments were investigated. MAE showed the strongest extraction power, shortest time and highest antioxidant activity. Maceration gave higher vanillin yields than UAE and PAE, similar antioxidant activity with UAE, but longer times than UAE and PAE. Overall odour intensity of different vanilla extracts obtained by UAE, PAE and MAE were similar, while higher than maceration extracts. Then, powered vanilla bean with a sample/solvent ratio of 4 g/100 mL was selected as the optimum condition for MAE. Next, compared with other three equations, two-site kinetic equation with lowest RMSD and highest R²(adj) was shown to be more suitable in describing the kinetics of vanillin extraction. By fitting the parameters C(eq), k₁, k₂, and f, a kinetics model was constructed to describe vanillin extraction in terms of irradiation power, ethanol concentration, and extraction time. PMID:24295676

  1. Introduction: Andrew Thomson and the Centre for Metalloprotein Spectroscopy and Biology at the University of East Anglia.

    PubMed

    Wilson, Michael T

    2008-12-01

    The present article briefly relates the early history and growth of the Centre for Metalloprotein Spectroscopy and Biology at UEA (University of East Anglia) under the joint directorship of A.J. Thomson and C. Greenwood, and charts the exceptional success that this centre has had in fostering bioinorganic chemistry in the U.K. and the impact that it has had internationally. PMID:19021505

  2. Mapping the nicking efficiencies of nickase R.BbvCI for side-specific LNA-substituted substrates using rolling circle amplification

    PubMed Central

    Wei, Hua; Zhao, Guojie; Hu, Tianyu; Tang, Suming; Jiang, Jiquan; Hu, Bo; Guan, Yifu

    2016-01-01

    We used a novel asymmetric cleavage analysis method based on rolling circle amplification (RCA) to determine the effects of LNA modification of substrate on the two subunits of R.BbvCI cleavage. We designed two sets of cleavage circular substrates by using two different ligation strategies and analyzed the single strand cleavage efficiency affected by different modification positions both from the cleaved strands and the uncleaved strands. Results showed that the effects of LNA on cleavage rates of modified strands and unmodified strands were both site-dependent. The Nb.BbvCI and Nt.BbvCI were affected by LNA modification in different way. Most of the modification positions showed strong inhibition of both of these two nickases cleavage. However, the modification in T3 position of bottom strand hardly affected both of the two nickases activities. The results suggested an intimated interaction between the two subunits of R.BbvCI, and the T3 position in bottom strand might be a less tight position which was hard to be disturbed. PMID:27582033

  3. Sealing of chromosomal DNA nicks during nucleotide excision repair requires XRCC1 and DNA ligase III alpha in a cell-cycle-specific manner.

    PubMed

    Moser, Jill; Kool, Hanneke; Giakzidis, Ioannis; Caldecott, Keith; Mullenders, Leon H F; Fousteri, Maria I

    2007-07-20

    Impaired gap filling and sealing of chromosomal DNA in nucleotide excision repair (NER) leads to genome instability. XRCC1-DNA ligase IIIalpha (XRCC1-Lig3) plays a central role in the repair of DNA single-strand breaks but has never been implicated in NER. Here we show that XRCC1-Lig3 is indispensable for ligation of NER-induced breaks and repair of UV lesions in quiescent cells. Furthermore, our results demonstrate that two distinct complexes differentially carry out gap filling in NER. XRCC1-Lig3 and DNA polymerase delta colocalize and interact with NER components in a UV- and incision-dependent manner throughout the cell cycle. In contrast, DNA ligase I and DNA polymerase epsilon are recruited to UV-damage sites only in proliferating cells. This study reveals an unexpected and key role for XRCC1-Lig3 in maintenance of genomic integrity by NER in both dividing and nondividing cells and provides evidence for cell-cycle regulation of NER-mediated repair synthesis in vivo. PMID:17643379

  4. Northern Hemisphere Wintertime Regional Trends Larissa Nazarenko and Nick Tausnev Columbia University/NASA Goddard Institute for Space Studies, New York, NY, USA

    NASA Astrophysics Data System (ADS)

    Nazarenko, L.; Tausnev, N.

    2013-12-01

    The amplified Arctic near-surface air temperature is one of the important features of climate change in recent decades. While there is attribution of the polar warming to human influence, winter-spring warming over northern areas of the Eurasia and the cooling of eastern Canada and southern Greenland during the 1980s and 1990s is argued to be largely associated with internal variability such as the positive phase of the Arctic Oscillation/North Atlantic Oscillation (AO/NAO). Although over the past 5-6 years the NAO is in more neutral state, some modeling studies suggest that the anthropogenic forcings, including the increased greenhouse gases and stratospheric ozone loss, may cause a higher frequency of the NAO positive phase. In addition to the amplified high latitude temperature increase, the global warming resulting from anthropogenic emissions of greenhouse gases may be linked with the effects of fluctuations of atmospheric circulation systems on precipitation distributions. The recent severe snow storms in the northwestern Europe, which is believed to be forced by the low NAO index conditions by the large extent, highlight the effect of natural fluctuations in atmospheric circulation systems on precipitation distribution. Using the GISS climate model with both anthropogenic (time-varying well-mixed greenhouse gases, ozone, tropospheric aerosols, stratospheric water vapor from methane oxidation, a parameterized indirect effect of aerosols on clouds, soot effect on snow and ice albedos, and land use changes) and natural forcings (changing volcanic aerosols, solar irradiance, and Earth orbital parameters), it is shown that human-induced increases in greenhouse gases in the last three decades of the 20th century, as well as anthropogenically forced scenarios for the twenty first century, have contributed to higher year-to year variability of the simulated NAO index reflected in fluctuations of atmospheric circulation systems, which in turn leads to the intensification/deficiency of precipitation in the different regions of the Northern Hemisphere. The model simulations produce a strengthened meridional pressure gradient over the North Atlantic during the last few decades of the twenties century, as well as in the projected twenty-first century warming scenarios. The NAO index exhibits a tendency towards the positive phase and enhancement of its intensity. The anomalously hot temperatures and heavy precipitation over Northern Europe, western Siberia, south-eastern America and northern Alaska are caused by positive NAO regime, while the negative NAO phase causes the extreme cold conditions over the northern Europe and increasing frequency of frozen precipitation over southern regions in Europe. Many proxy indicators in paleoclimate variability reconstructions and different studies of past, present and future high-risk events with global and regional models indicate that Europe will face major changes in extreme events, such as heat waves, heavy winter precipitation, extreme wind storms. RCP8.5 scenario with the strongest greenhouse warming presents cooling over major continental areas under the negative NAO phase that confirms enhanced variability and intensity of climate variability under extreme warming conditions.

  5. Nickel(I) and nickel(III) complexes of substituted tetraaza macrocycles formed by pulse radiolysis and electrochemistry of nickel(II) precursors

    SciTech Connect

    Bernhardt, P.V.; Lawrance, G.A.; Sangster, D.F.

    1988-11-02

    The square-planar nickel(II) complexes of the ligands 8-methyl-8-nitro-1,3,6,10,13,15-hexaazatricyclo(13.1.1.1/sup 13,15/)octadecane, 8-amino-8-methyl-1,3,6,10,13,15-hexaazatricyclo(13.1.1.1/sup 13,15/)octadecane, 3,7-bis(2-aminoethyl)-1,3,5,7-tetraazabicyclo(3.3.1)nonane, and 9-methyl-9-nitro-1,4,7,11-tetraazacyclotridecane (I-IV) react rapidly with hydroxyl radicals and aquated electrons (e/sub aq/). The initial transient products of these reactions decay via first-order kinetics within a few milliseconds in neutral aqueous solution at 22/degrees/C in all cases. Electronic spectra and decay rate constants, as well as formation rate constants, are reported for all transients. Reaction of the nitro-substituted complexes with e/sub aq/ led to electron addition to the nitro group rather than to the metal center; otherwise, a Ni/sup I/ transient is observed. Following reaction with OH, the product of the initial decay remains a Ni/sup III/ species. This is more long-lived, and stabilization of Ni/sup III/ by axial coordination of the pendant amine in II is indicated. No notable stabilization of Ni/sup I/ or Ni/sup III/ from the presence of the bicyclic azamethylene football in I-III occurs. Cyclic voltammetry in acetonitrile identified both one-electron oxidation and one-electron reduction processes for the nickel(II) complexes, as well as nitro group reduction, where this group was pendant to the macrocycle. 34 references, 3 figures, 3 tables.

  6. NIOSH testimony on Kuwait before the subcommittee on hospitals and health care, committee on veterans' affairs by J. S. Andrews, September 16, 1992

    SciTech Connect

    Not Available

    1992-09-16

    The testimony summarizes potential adverse health effects related to service in the Persian Gulf as presented by the Department of Health and Human Services. An estimated 9,000 workers from 43 different countries battled the burning oil wells in Kuwait from February 1991 through early November 1991 when the last was capped. Exposures and health effects in US military personnel, Kuwaiti citizens, and fire fighters were described. The hazards to the soldiers were largely dependent on the concentration of the pollutants in the air near the camps. Fortunately, the plume from the fires rose up to 10,000 and 12,000 feet, mixed with the air and then dispersed for several thousand miles downwind over a period of several weeks. The particles and gases contained in the plume were diluted as the plume travelled. Even so, some minor respiratory problems were present among the soldiers. Some of the hydrocarbons measured at low concentrations have been shown to produce cancer in laboratory animals only when present at higher levels of exposure. Based on the exposure information gathered, the author concludes that there will not likely be a detectable increase in lung cancer in Gulf War Veterans as a result of the oil well fires.

  7. Environmental magnetic implications of greigite (Fe3S4) formation in a 3 m.y. lake sediment record from Butte Valley, northern California Andrew

    USGS Publications Warehouse

    Roberts, P.; Reynolds, R.L.; Verosub, K.L.; Adam, D.P.

    1996-01-01

    Authigenic greigite (Fe3S4) has been identified in several horizons of lake beds in a 102-m core from Butte Valley, northern California, using mineral magnetic methods and x-ray diffraction analysis. The presence of greigite has several implications for the paleoenvironmental record from Butte Valley. First, its occurrence in 2.5 - 3.0 Ma strata confirms that greigite can persist in the geological record for long periods of time. Second, the detrital mineral magnetic record may be partially obscured by the presence of authigenic greigite and care must be taken in interpreting magnetic variations in the greigite-bearing zones as paleoclimate proxies. Third, differences in the timing of remanence acquisition for authigenic and detrital phases may compromise studies of high-frequency geomagnetic field variations. Fourth, greigite may also be significant as a paleoenvironmental indicator of lake and sediment chemistry. The magnetic detection of greigite may therefore provide important information about palcolimnological conditions. Copyright 1996 by the American Geophysical Union.

  8. Depositional and diagenetic controls on porosity permeability and oil production in McFarland/Magutex (Queen) reservoirs, Andrews County, west Texas

    SciTech Connect

    Holtz, M.H. )

    1991-03-01

    The McFarland/Magutex Queen reservoir complex lies along the northeastern edge of the Central basin platform in the west Texas Permian basin and produces oil from the Permian Queen Formation. Current production from this complex totals 42 million stock-tank barrels (MMSTB) of an estimated 219 MMSTB of original oil in place, with an estimated 90 MMSTB of remaining mobile oil (RMO). The gross pay interval contains two parasequences consisting of progradational, 30-ft-thick, upward-shoaling facies packages. Facies include shoreface, mixed tidal channel and intertidal flat, and supratidal. Elongate shoreface facies are characterized by poorly consolidated, massive to thinly laminated sandstones. The supratidal facies, which act as permeability barriers, are characterized by algal-laminated dolostone and nodular, laminated, and massive anhydrite containing halite and gypsum pseudomorphs. Highest production and the largest amount of the 90 MMSTB of RMO is associated with the shoreface and tidal-channel facies. Bulk pore volume storage capacity and permeability are also highest within these two facies. Sandstones are arkosic, containing anhydrite and dolomite cements. Accessory minerals are clays, authigenic feldspar, and dolomite. Three main pore types are recognized: interparticle, moldic and intraconstituent, and micropores. Moldic and intraconstituent porosity is associated with leached feldspars and anhydrite cement dissolution. Microporosity is associated with syndepositional, grain-coating corrensite, dissolution-enhanced feldspar cleavage planes, and authigenic multifaceted dolomite. Microporosity derived from clays and dolomite is formed preferentially in tidal-channel and intertidal flat facies.

  9. 78 FR 76812 - In the Matter of: Andrew Vincent O'Donnell, Inmate Number-62355-019, USP Atlanta, U.S...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-12-19

    ... (78 FR 49107 (August 12, 2013)), has continued the Regulations in effect under the International... known and unknown, knowingly and willfully exporting and attempting to export EOTech Holographic...

  10. Chemometric analysis of chromatographic fingerprints shows potential of Cyclopia maculata (Andrews) Kies for production of standardized extracts with high xanthone content.

    PubMed

    Schulze, Alexandra E; de Beer, Dalene; de Villiers, André; Manley, Marena; Joubert, Elizabeth

    2014-10-29

    Cyclopia species are used for the production of honeybush tea and food ingredient extracts associated with many health benefits. A species-specific high-performance liquid chromatography (HPLC) method for Cyclopia maculata, developed and validated, allowed quantification of the major compounds in extracts from "unfermented" and fermented C. maculata. Two xanthones were tentatively identified for the first time in a Cyclopia species, whereas an additional four compounds were tentatively identified for the first time in C. maculata. "Fermentation" (oxidation) decreased the content of all compounds, with the exception of vicenin-2. Similarity analysis of the chromatographic fingerprints of unfermented C. maculata aqueous extracts showed extremely low variation (r ≥ 0.97) between samples. Some differences between wild-harvested and cultivated seedling plants were, however, demonstrated using principal component analysis. Quantitative data of selected compounds confirmed the low level of variation, making this Cyclopia species ideal for the production of standardized food ingredient extracts. PMID:25329526

  11. One World, Many Cultures. Papers from the International Conference on Adult Education and the Arts (4th, St. Andrews, Scotland, July 10-14, 1995).

    ERIC Educational Resources Information Center

    Jones, David, Ed.; McConnell, Bridget, Ed.; Normie, Gerald, Ed.

    The following papers are included: "Foreword" (Bridget McConnell); "Introduction" (David J. Jones); opening addresses by George Robertson MP, Shadow Scottish Secretary, and by Charlie McConnell, Executive Director, Scottish Community Education Council; and speech by Christine Hamilton, Deputy Director, Scottish Arts Council; "Keynote Speech:…

  12. Society News: RAS Awards 2012; Prof. Andy Fabian; Prof. John C Brown; Prof. Andrew Fazakerley; Dr. Mike Irwin; Joss Bland-Hawthorn

    NASA Astrophysics Data System (ADS)

    2012-02-01

    Each year the RAS recognizes outstanding achievement in astronomy and geophysics by the award of medals and prizes. Candidates are nominated by Fellows and the awards made by a committee of Fellows, ensuring that these scientists have earned the respect and admiration of their peers in the research community.

  13. Paeonol Protects Memory after Ischemic Stroke via Inhibiting β-Secretase and Apoptosis.

    PubMed

    Su, Shan-Yu; Cheng, Chin-Yi; Tsai, Tung-Hu; Hsieh, Ching-Liang

    2012-01-01

    Poststroke dementia commonly occurs following stroke, with its pathogenesis related to β-amyloid production and apoptosis. The present study evaluate the effects of paeonol, one of the phenolic phytochemicals isolated from the Chinese herb Paeonia suffruticosa Andrews (MC), on protection from memory loss after ischemic stroke in the subacute stage. Rats were subjected to transient middle cerebral artery occlusion (tMCAo) with 10 min of ischemia. The data revealed that paeonol recovered the step-through latency in the retrieval test seven days after tMCAo, but did not improve the neurological deficit induced by tMCAo. Levels of Amyloid precursor protein (APP)- and beta-site APP cleaving enzyme (BACE; β-secretase)-immunoreactive cells, and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells decreased in the paeonol-administered group. Western blotting revealed decreased levels of Bax protein in mitochondria and apoptosis-inducing factor (AIF) in cytosol following paeonol treatment. In conclusion, we speculate that paeonol protected memory after ischemic stroke via reducing APP, BACE, and apoptosis. Supression the level of Bax and blocking the release of AIF into cytosol might participate in the anti-apoptosis provided by paeonol. PMID:22474531

  14. Fabrication of a Guarded-Hot-Plate Apparatus for Use over an Extended Temperature Expansion Symposium in St. Andrews-by-the-Sea, New Brunswick, Canada 6/26-29/2005

    SciTech Connect

    R.R. Zarr, D.R. Flynn, J. W. Hettenhouser

    2005-07-01

    NIST has initial a program to design and fabricate a 500 mm diameter guarded-hot-plate apparatus for the eventual development of thermal insulation reference materials for greater temperature and pressure ranges than are currently available.

  15. Letter to the Editor Re: Andrew Turner, Emily R. Kearl, Kevin R. Solman Lead and other toxic metals in playground paints from South West England Science of the Total Environment 544 (2016) 460-466.

    PubMed

    Gottesfeld, Perry; Jacobs, David E

    2016-08-15

    Turner's paper emphasizes "oral bioaccessibility" instead of focusing solely on total lead content. There is no evidence that solubility testing for lead levels in paint correlates with absorption or blood lead levels in exposed children. There are many considerations in determining exposure hazards to paint that are not evaluated in assessing solubility. Although we strongly support the conclusions and recommendations of the study, we are concerned that by reporting "oral bioaccessibility" others will focus on solubility in developing regulatory standards for lead levels in paint or in conducting exposure assessments. Standards for lead in paint should continue to be based on total lead content, not "oral bioaccessibility." PMID:27260620

  16. Creating a multi-gas proxy for Delta 14C and atmospheric fossil fuel-CO2. Kevin Coakley, John Miller , Scott Lehman, Stephen Montzka, Colm Sweeney, Arlyn Andrews , Ben Miller

    NASA Astrophysics Data System (ADS)

    Coakley, K. J.; Miller, J. B.; Lehman, S.; Montzka, S. A.; Andrews, A. E.; Miller, B. R.

    2013-12-01

    The C14:C12 ratio of atmospheric CO2 (expressed as Delta 14C) is the gold standard measurement to derive the portion of observed atmospheric CO2 gradients resulting from combustion of fossil fuels (CO2-ff). This is because fossil fuels are devoid of 14C, unlike all other sources and sinks that impact atmospheric Delta14C. With enough 14C measurements, independent, 'top-down', estimation of US fossil fuel-CO2 emissions should be possible. However, our ability to make carbon-14 measurements is severely constrained by cost, accessibility to accelerator mass spectrometers (AMS) and the volume of air required to make high precision (~0.2 %) measurements of 14CO2 (mixing ratio is ~ 4e-16 mol/mol). Thus, Delta 14C is currently measured in only a small subset of NOAA/ESRL Global Monitoring Division (GMD) tall-tower and aircraft air samples. Here, we present a Projection Pursuit Regression (PPR) model to predict CO2-ff measured at different times and altitudes in terms of surrogate gases that are more widespread and relatively inexpensive to measure. This method would, in effect, allow expansion of Delta 14C measurements by factor of ~3 or 4 throughout North America. To create a proxy for CO2-ff, we take advantage of the observed correlations between (Delta 14C-derrived) CO2-ff and regional-scale enhancements of a wide array of anthropogenic gases, like CO, SF6, and halo- and hydro-carbons. We select the complexity and form of the PPR model by cross validation where validation data prediction error is minimized. In cross validation, the prediction model is based on the training data and not the validation data. We quantify prediction model performance with test data excluded from the model development process. According to cross validation, the PPR model is superior to a simpler linear model. Comparison with test CO2-ff data shows that CO2-ff can be predicted with a root mean square error of 1.1 ppm, only slightly higher than the Delta 14C-precision limit for CO2-ff of 1 ppm. Applying the PPR model to the large NOAA/ESRL/GMD database of surrogate gases, we can create a large set of synthetic CO2-ff data for the US. Finally, we will discuss the application of this large data set of lower-precision CO2-ff to top down determination of US fossil fuel CO2 emissions by presenting results from an Observation System Simulation Experiment (OSSE).

  17. Installation-Restoration Program. Phase 2. Confirmation/quantification. Stage 1. Problem confirmation study: Otis Air National Guard Base, Massachusetts, Air National Guard Support Center, Andrews Air Force Base, Maryland. Final technical report, November 1983-July 1985

    SciTech Connect

    Kraybill, R.L.; Smart, G.R.; Bopp, F.

    1985-09-04

    A Problem Confirmation Study was performed at seven sites on Otis Air National Guard Base: the Current and Former Training Areas, the Base Landfill, the Nondestructive Inspection Laboratory, the Fuel Test Dump Site, the Railyard Fuel Pumping Station, and the Petrol Fuel Storage Area. The field investigation was conducted in two stages, in November 1983 through January 1984, and in October through December 1984. Resampling was performed at selected locations in April and July 1985. A total of 11 monitor wells were installed and sampled and test-pit investigations were conducted at six sites. In addition, the contents of a sump tank, and two header pipes for fuel-transmission lines were sampled. Analytes included TOC, TOX, cyanide, phenols, Safe Drinking Water metals, pesticides and herbicides, and in the second round, priority-pollutant volatile organic compounds and a GC fingerprint scan for fuel products. On the basis of the field-work findings, it is concluded that, to date, water-quality impacts on ground water from past activities have been minimal.

  18. The Role of Science in Managed Aquifer Recharge--the Equus Beds aquifer near Wichita, Kansas Andrew Ziegler, Director Brian Kelly, Office Chief Michael Jacobs, Manager of Water Planning and Production Debra Ary, Engineer, Water Systems Planning (Invited)

    NASA Astrophysics Data System (ADS)

    Ziegler, A. C.; Jacobs, M.; Ary, D.; Kelly, B.

    2013-12-01

    Data collection and interpretation using statistical, geochemical, and numerical simulation tools are essential parts of a long-term cooperative study between the city of Wichita, U.S. Geological Survey, and others to describe water quantity and quality conditions in a 165 square-mile part of the Equus Beds aquifer and Arkansas and Little Arkansas Rivers. The Equus Beds aquifer, eastern part of the High Plains Aquifer in south-central Kansas, is a vital water resource for agriculture and city of Wichita. Withdrawals for public supply began in the 1940s and agricultural irrigation began in the 1950-60s. These withdrawals led to water-level declines of up to 40 feet (historic low in 1993), a storage loss of 250,000 acre feet compared to predevelopment, and may enhance movement of chloride contamination from a past oilfield disposal area near Burrton and from natural chloride along the Arkansas River. Monitoring data and modeling show chloride near Burrton moved about 3 miles in 45 years, is about 1 mile away from the nearest public supply wells, and will continue to move for decades to centuries making the water unusable for irrigation or water supply without treatment. These concerns led to development of Wichita's 1993 integrated local water-supply plan that increased use of Cheney Reservoir and implemented aquifer storage and recovery (ASR) within the aquifer using high flows from the Little Arkansas River. ASR benefits include replacing depleted storage and slowing chloride movement. Decreased withdrawals, increased precipitation, and artificial recharge increased water levels and added 100,000 acre feet of storage through 2010, but drought since 2011 has increased withdrawals. A calibrated model will be used to simulate transport of chloride under several withdrawal scenarios using MODFLOW coupled with SEAWAT. Since 1995, water-quality data collection for more than 400 organic and inorganic compounds in surface water, treated source water for artificial recharge, and groundwater identified indicator bacteria, atrazine, chloride, sodium, nitrate, arsenic, iron, and manganese as constituents of concern exceeding water-quality criteria in baseline samples. Techniques were developed to estimate Little Arkansas River water quality in real-time for treatment. Geochemical modeling using PHREEQC and PHAST shows that groundwater quality is not changed if groundwater and recharge water are of similar redox potential. If different, calcite or metal hydroxides may precipitate and decrease water infiltration. A network of 38 locations with shallow and deep wells characterizes the recharge quantities and qualities for the city of Wichita to withdraw when needed from storage. Through 2013, the Demonstration project and Phase 1 and 2 facilities (capacity 40 MGD) have artificially recharged about 2 billion gallons. Total construction costs are about $300,000,000. Data-collection, interpretative geochemical and numerical simulations and water-quality transport modeling tools developed in the past 70 years are a scientific foundation to effectively and objectively manage this aquifer system.

  19. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  20. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  1. 20 CFR 416.1232 - Replacement of lost, damaged, or stolen excluded resources.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... Exception: For victims of Hurricane Andrew only, the extension period for good cause may be extended for up... additional extension under the Hurricane Andrew provision) may be extended for a reasonable period up to...

  2. Alternative nucleophilic substrates for the endonuclease activities of human immunodeficiency virus type 1 integrase

    SciTech Connect

    Ealy, Julie B.; Sudol, Malgorzata; Krzeminski, Jacek; Amin, Shantu; Katzman, Michael

    2012-11-10

    Retroviral integrase can use water or some small alcohols as the attacking nucleophile to nick DNA. To characterize the range of compounds that human immunodeficiency virus type 1 integrase can accommodate for its endonuclease activities, we tested 45 potential electron donors (having varied size and number or spacing of nucleophilic groups) as substrates during site-specific nicking at viral DNA ends and during nonspecific nicking reactions. We found that integrase used 22 of the 45 compounds to nick DNA, but not all active compounds were used for both activities. In particular, 13 compounds were used for site-specific and nonspecific nicking, 5 only for site-specific nicking, and 4 only for nonspecific nicking; 23 other compounds were not used for either activity. Thus, integrase can accommodate a large number of nucleophilic substrates but has selective requirements for its different activities, underscoring its dynamic properties and providing new information for modeling and understanding integrase.

  3. Strength and Fatigue of NT551 Silicon Nitride and NT551 Diesel Exhaust Valves

    SciTech Connect

    Andrews, M.J.; Wereszczak, A.A.; Kirkland, T.P.; Breder, K.

    2000-02-01

    The content of this report is excerpted from Mark Andrew's Ph.D. Thesis (Andrews, 1999), which was funded by a DOEYOTT High Temperature Materials Laboratory Graduate Fellowship. It involves the characterization of NT551 and valves fabricated with it. Greater detail of the described issues may be found in that reference or through communications with Andrew Wereszczak.

  4. The Inhibitory Effect of Non-Substrate and Substrate DNA on the Ligation and Self-Adenylylation Reactions Catalyzed by T4 DNA Ligase

    PubMed Central

    Bauer, Robert J.; Evans, Thomas C.; Lohman, Gregory J. S.

    2016-01-01

    DNA ligases are essential both to in vivo replication, repair and recombination processes, and in vitro molecular biology protocols. Prior characterization of DNA ligases through gel shift assays has shown the presence of a nick site to be essential for tight binding between the enzyme and its dsDNA substrate, with no interaction evident on dsDNA lacking a nick. In the current study, we observed a significant substrate inhibition effect, as well as the inhibition of both the self-adenylylation and nick-sealing steps of T4 DNA ligase by non-nicked, non-substrate dsDNA. Inhibition by non-substrate DNA was dependent only on the total DNA concentration rather than the structure; with 1 μg/mL of 40-mers, 75-mers, or circular plasmid DNA all inhibiting ligation equally. A >15-fold reduction in T4 DNA ligase self-adenylylation rate when in the presence of high non-nicked dsDNA concentrations was observed. Finally, EMSAs were utilized to demonstrate that non-substrate dsDNA can compete with nicked dsDNA substrates for enzyme binding. Based upon these data, we hypothesize the inhibition of T4 DNA ligase by non-nicked dsDNA is direct evidence for a two-step nick-binding mechanism, with an initial, nick-independent, transient dsDNA-binding event preceding a transition to a stable binding complex in the presence of a nick site. PMID:26954034

  5. IMPACT OF CRITICAL ANION SOIL SOLUTION CONCENTRATION ON ALUMINUM ACTIVITY IN ALPINE TUNDRA SOIL Andrew Evans, Jr.1 , Michael B. Jacobs2, and Jason R. Janke1, (1) Metropolitan State University of Denver, Dept. of Earth and Atmospheric Sciences, (2) Dept. of Chemistry, Denver, CO, United States.

    NASA Astrophysics Data System (ADS)

    Evans, A.

    2015-12-01

    Soil solution anionic composition can impact both plant and microbial activity in alpine tundra soils by altering biochemical cycling within the soil, either through base cation leaching, or shifts in aluminum controlling solid phases. Although anions play a critical role in the aqueous speciation of metals, relatively few high altitude field studies have examined their impact on aluminum controlling solid phases and aluminum speciation in soil water. For this study, thirty sampling sites were selected on Trail Ridge Road in Rocky Mountain National Park, Estes Park, CO, and sampled during July, the middle of the growing season. Sampling elevations ranged from approximately 3560 - 3710 m. Soil samples were collected to a depth of 15.24 cm, and the anions were extracted using a 2:1 D.I. water to soil ratio. Filtered extracts were analyzed using IC and ICP-MS. Soil solution NO3- concentrations were significantly higher for sampling locations east of Iceberg Pass (EIBP) (mean = 86.94 ± 119.8 mg/L) compared to locations west of Iceberg Pass (WIBP) (mean 1.481 ± 2.444 mg/L). Both F- and PO43- soil solution concentrations, 0.533 and 0.440 mg/L, respectively, were substantially lower, for sampling sites located EIBP, while locations WIBP averaged 0.773 and 0.829 mg/L respectively, for F- and PO43-. Sulfate concentration averaged 3.869 ± 3.059 mg/L for locations EIBP, and 3.891 ± 3.1970 for locations WIBP. Geochemical modeling of Al3+ in the soil solution indicated that a suite of aluminum hydroxyl sulfate minerals controlled Al3+ activity in the alpine tundra soil, with shifts between controlling solid phases occurring in the presence of elevated F- concentrations.

  6. Surfaces are different: A perspective on structural, energetic and electronic properties of (001) surfaces of alkaline earth metal oxides as calculated with hybrid density functional theory by Andrew J. Logsdail, David Mora-Fonz, David O. Scanlon, C. Richard A. Catlow, Alexey A. Sokol

    NASA Astrophysics Data System (ADS)

    Illas, Francesc; Pacchioni, Gianfranco

    2015-12-01

    Surfaces are difficult objects and Surface Science readers know this very well; surfaces exposed to vacuum are often unstable and gain stabilization through relaxation or reconstruction. Surfaces display a positive work function, the extra energy required for a free electron in the solid to go through the surface dipole layer to the vacuum; surfaces are also behind the band bending phenomenon exhibited by semiconductors; i.e. the band gap at the surface is usually smaller than for the bulk material [1].

  7. United States History Simulations: 1787-1868: Constitution Convention, Missouri Compromise, Treaty of Guadalupe Hidalgo, The Compromise of 1850, The Kansas/Nebraska Act, Southern Secession from the Union, and the Impeachment and Trial of Andrew Johnson. ETC Simulations Number Two.

    ERIC Educational Resources Information Center

    Hostrop, Richard W.

    This book presents simulation activities for significant events in U.S. history from 1787-1868. Intended for student involvement, the simulations require student research and practice in order to carry out the designated roles. The simulation and role play serve to involve the students actively in their learning, using both the affective and…

  8. Comment on "Nanosecond laser textured superhydrophobic metallic surfaces and their chemical sensing applications" by Duong V. Ta, Andrew Dunn, Thomas J. Wasley, Robert W. Kay, Jonathan Stringer, Patrick J. Smith, Colm Connaughton, Jonathan D. Shephard (Appl. Surf. Sci. 357 (2015) 248-254)

    NASA Astrophysics Data System (ADS)

    Boinovich, L. B.; Emelyanenko, A. M.; Emelyanenko, K. A.; Domantovsky, A. G.; Shiryaev, A. A.

    2016-08-01

    Nowadays the problem of design of durable ecologically friendly superhydrophobic surfaces is of great importance for science and technology. A recent paper in Applied Surface Science reports the method of fabricating the superhydrophobic metallic surfaces by infrared nanosecond laser surface texturing without using hydrophobic agents. Since this method of surface texturing can be considered as one of the most suitable for various industrial applications, the nature of superhydrophobic state of surfaces produced by laser texturing in the abovementioned paper deserves to be analyzed in detail. Authors of the commented paper attributed the change in wettability to the partial deoxidation of CuO into Cu2O on the surface during storage in atmosphere. However, such interpretation of the results contradicts to the basic notions in the theory of wetting and to more accurate and detailed data. In our Comment we discuss these contradictions point by point.

  9. Teaching and Learning Astronomy

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay; Percy, John

    2005-12-01

    Preface; Part I. Astronomy in the Curriculum Around the World: Preface; 1. Why astronomy is useful and should be included in the school curriculum John R. Percy; 2. Astronomy and mathematics education Rosa M. Ros; 3. Astronomy in the curriculum around the world; 4. Engaging gifted science students through astronomy Robert Hollow; 5. Poster highlights: astronomy in the curriculum around the world; Part II. Astronomy Education Research: Preface; 6. Astronomy education research down under John M. Broadfoot and Ian S. Ginns; 7. A contemporary review of K-16 astronomy education research Janelle M. Bailey and Timothy F. Slater; 8. Implementing astronomy education research Leonarda Fucili; 9. The Astronomy Education Review: report on a new journal Sidney C. Wolff and Andrew Fraknoi; 10. Poster highlights: astronomy education research; Part III. Educating Students: Preface; 11. Textbooks for K-12 astronomy Jay M. Pasachoff; 12. Distance/internet astronomy education David H. McKinnon; 13. Educating students with robotic telescopes - open discussion; 14. Poster highlights - educating students; Part IV. Educating teachers: Preface; 15. Pre-service astronomy education of teachers Mary Kay Hemenway; 16. In-service education of teachers Michèle Gerbaldi; 17. Poster highlights: educating teachers; Part V. Astronomy and Pseudoscience: Preface; 18. Astronomy, pseudoscience and rational thinking Jayant V. Narlikar; 19. Astronomical pseudosciences in North America John R. Percy and Jay M. Pasachoff; Part VI. Astronomy and Culture: Preface; 20. Teaching astronomy in other cultures: archeoastronomy Julieta Fierro; 21. Poster highlights: astronomy and culture; Part VII. Astronomy in Developing Countries: Preface; 22. Astronomy Curriculum for developing countries Case Rijsdijk; 23. Science education resources for the developing countries James C. White II; Part VIII. Public Outreach in Astronomy: Preface; 24. What makes informal education programs successful? Nahide Craig and Isabel

  10. Teaching and Learning Astronomy

    NASA Astrophysics Data System (ADS)

    Pasachoff, Jay; Percy, John

    2009-07-01

    Preface; Part I. Astronomy in the Curriculum Around the World: Preface; 1. Why astronomy is useful and should be included in the school curriculum John R. Percy; 2. Astronomy and mathematics education Rosa M. Ros; 3. Astronomy in the curriculum around the world; 4. Engaging gifted science students through astronomy Robert Hollow; 5. Poster highlights: astronomy in the curriculum around the world; Part II. Astronomy Education Research: Preface; 6. Astronomy education research down under John M. Broadfoot and Ian S. Ginns; 7. A contemporary review of K-16 astronomy education research Janelle M. Bailey and Timothy F. Slater; 8. Implementing astronomy education research Leonarda Fucili; 9. The Astronomy Education Review: report on a new journal Sidney C. Wolff and Andrew Fraknoi; 10. Poster highlights: astronomy education research; Part III. Educating Students: Preface; 11. Textbooks for K-12 astronomy Jay M. Pasachoff; 12. Distance/internet astronomy education David H. McKinnon; 13. Educating students with robotic telescopes - open discussion; 14. Poster highlights - educating students; Part IV. Educating teachers: Preface; 15. Pre-service astronomy education of teachers Mary Kay Hemenway; 16. In-service education of teachers Michèle Gerbaldi; 17. Poster highlights: educating teachers; Part V. Astronomy and Pseudoscience: Preface; 18. Astronomy, pseudoscience and rational thinking Jayant V. Narlikar; 19. Astronomical pseudosciences in North America John R. Percy and Jay M. Pasachoff; Part VI. Astronomy and Culture: Preface; 20. Teaching astronomy in other cultures: archeoastronomy Julieta Fierro; 21. Poster highlights: astronomy and culture; Part VII. Astronomy in Developing Countries: Preface; 22. Astronomy Curriculum for developing countries Case Rijsdijk; 23. Science education resources for the developing countries James C. White II; Part VIII. Public Outreach in Astronomy: Preface; 24. What makes informal education programs successful? Nahide Craig and Isabel

  11. STS-134 Tribute to Endeavour

    NASA Video Gallery

    STS-134 Commander Mark Kelly pays tribute to space shuttle Endeavour and the spacecraft's contribution to human spaceflight. Mission specialists Andrew Feustel, Mike Fincke, Roberto Vittori, Greg C...

  12. 76 FR 28421 - Proposed Information Collection; Comment Request; Marine Recreational Fisheries Statistics Survey

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-17

    ... Recreational Fisheries Statistics Survey AGENCY: National Oceanic and Atmospheric Administration (NOAA). ACTION... Service, Fisheries Statistics Division, Phone: (301) 713-2328 or Rob.Andrews@noaa.gov ....

  13. 78 FR 45535 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-29

    .... Hughes, Jr., Jacksonville, Florida; J. Malcolm Jones, Jr., Jacksonville, Florida; James T. Katsur, Longwood, Florida; W. Andrew Krusen, Jr., Tampa, Florida; Allan S. Martin, Tampa, Florida; Linda C....

  14. 77 FR 77076 - Environmental Impacts Statements; Notice of Availability

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ... Restoration Project, Andrew Pickens Ranger District, Sumter National Forest, Oconee County, CA, Comment Period..., Ice Age Complex at Cross Plains General Management Plan, Implementation, Ice Age National Scenic...

  15. Accentuating the Positive: "Can't" Doesn't Cut It

    ERIC Educational Resources Information Center

    Hyndman, Amy

    2011-01-01

    Nick is the author's first-born child. He was born healthy in 1993. Within days, however, there would be medical complications resulting in brain damage, seizures, medical issues, a diagnosis of cerebral palsy at four months of age, and later, a diagnosis of bi-lateral progressive hearing loss at eight years of age. Nick's parents have been told…

  16. E-Books Mediator: Nicholas Bogaty--Open Ebook Forum, New York

    ERIC Educational Resources Information Center

    Library Journal, 2004

    2004-01-01

    This article is about the work of Nick Bogaty, executive director of the Open eBook Forum. Nick Bogaty is not a librarian, but he plays nicely with them, along with publishers, hardware manufacturers, software producers, database vendors, and disability rights advocates. All are groups that share an interest in making e-books work for their…

  17. Analysis of the Latin Square Task with Linear Logistic Test Models

    ERIC Educational Resources Information Center

    Zeuch, Nina; Holling, Heinz; Kuhn, Jorg-Tobias

    2011-01-01

    The Latin Square Task (LST) was developed by Birney, Halford, and Andrews [Birney, D. P., Halford, G. S., & Andrews, G. (2006). Measuring the influence of cognitive complexity on relational reasoning: The development of the Latin Square Task. Educational and Psychological Measurement, 66, 146-171.] and represents a non-domain specific,…

  18. ES Review: Selections from 2009 and 2010

    ERIC Educational Resources Information Center

    Smiles, Robin, Ed.

    2010-01-01

    This fourth edition of the "ES Review" brings together, in one setting, some of the best work from 2009-10. It features: (1) Teacher Quality (Teachers at Work: Improving Teacher Quality Through School Design (Elena Silva); Understanding Teachers Contracts (Andrew J. Rotherham); How Teachers Unions Lost the Media (Richard Whitmire and Andrew J.…

  19. 78 FR 25242 - Delegation of New Source Performance Standards and National Emission Standards for Hazardous Air...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-04-30

    ... Standards for Hazardous Air Pollutants for the States of Arizona, California, and Nevada AGENCY... Source Performance Standards (NSPS) and National Emission Standards for Hazardous Air Pollutants (NESHAP...: steckel.andrew@epa.gov . 3. Mail or deliver: Andrew Steckel (Air-4), U.S. Environmental Protection...

  20. Huggins, martians and exoplanets

    NASA Astrophysics Data System (ADS)

    2013-02-01

    The RAS Ordinary A & G meeting in November included the Gerald Whitrow Lecture by Prof. Andrew Liddle, while in December Prof. Andrew Collier Cameron gave the George Darwin Lecture. Both will be reported in future issues. Here Sue Bowler summarizes the other talks.

  1. Technology Counts 2007: A Digital Decade

    ERIC Educational Resources Information Center

    Education Week, 2007

    2007-01-01

    "Technology Counts 2007" looks back, and ahead, after a decade of enormous upheaval in the educational technology landscape. This special issue of "Education Week" includes the following articles: (1) A Digital Decade; (2) Getting Up to Speed (Andrew Trotter); (3) E-Rate's Imprint Seen in Schools (Andrew Trotter); (4) Teaching Assistants (Rhea R.…

  2. Why Play "I spy" When You Can Do Mathematics?

    ERIC Educational Resources Information Center

    Andrews, Robert; Andrews, Paul

    2007-01-01

    This article presents a story of Robert Andrew's mental engagement with mathematics. It has been jointly written by Robert, who is a Y8 student, and his father, Paul Andrews, who is a mathematics educator. It starts with Robert who presents the context for the story and is followed by Paul's commentary which draws on what Robert has written.…

  3. Presidents Talking about the Presidency.

    ERIC Educational Resources Information Center

    Twohig, Dorothy; And Others

    1997-01-01

    Presents brief excerpts from the presidential papers of George Washington, Thomas Jefferson, Andrew Jackson, James Polk, Andrew Johnson, Ulysses S. Grant, and Dwight D. Eisenhower. The excerpts address such matters as voting rights, limited government, political appointments, national security, and the balance of power. (MJP)

  4. The Evolution of an Adolescent Literacy Program: A Foundation's Journey

    ERIC Educational Resources Information Center

    Henriquez, Andres

    2005-01-01

    The Carnegie Corporation of New York is associated with the very foundations of literacy going back to the philanthropy of Andrew Carnegie himself and of the Corporation in its early years. Andrew Carnegie's legacy includes over 2,500 free public libraries that he saw as a link "bridging ignorance and education." In fact, Carnegie, the man,…

  5. Creating a Shared Definition of Good and Bad Writing through Revision Strategies

    ERIC Educational Resources Information Center

    Baer, Allison L.

    2008-01-01

    This article describes how the author, in an effort to build some common language about "good writing" and to teach effective revision strategies, involved her students in an action research project using the Worse/Better Writing Strategy developed by Andrews-Beck. In her dissertation, Andrews-Beck described a worse/better writing strategy that…

  6. Algebra: How Is It for You?

    ERIC Educational Resources Information Center

    Rickard, Caroline

    2008-01-01

    Shortly after starting work for the University of Chichester in the School of Teacher Education, the author was planning a session relating to algebra and found herself inspired by an article in MT182: "Algebraic Infants" by Andrews and Sayers (2003). Based on the making of families of "Multilink" animals, Andrews and Sayers (2003) claim that…

  7. An antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata.

    PubMed

    Begam, M; Narwal, S; Roy, S; Kumar, S; Lodha, M L; Kapoor, H C

    2006-01-01

    An antiviral protein named CCP-27 was purified from the leaves of Celosia cristata at the post-flowering stage by anion-exchange, cation-exchange, and gel-filtration chromatography. It exhibited resistance against sunnhemp rosette virus in its test host Cyamopsis tetragonoloba. It also exhibited deoxyribonuclease activity against supercoiled pBlueScript SK+ plasmid DNA. It was found to nick supercoiled DNA into nicked circular form at lower protein concentration followed by nicked to linear form conversion at higher protein concentration. CCP-27 also possesses strong ribonuclease activity against Torula yeast rRNA. PMID:16487067

  8. Site-specifically modified oligodeoxyribonucleotides as templates for Escherichia coli DNA polymerase I.

    PubMed Central

    O'Connor, D; Stöhrer, G

    1985-01-01

    Oligodeoxyribonucleotides with site-specific modifications have been used as substrates for Escherichia coli DNA polymerase I holoenzyme and Klenow fragment. Modifications included the bulky guanine-8-aminofluorene adduct and a guanine oxidation product resembling the product of photosensitized DNA oxidation. By a combination of primers and "nick-mers", conditions of single-strand-directed DNA synthesis and nick-translation could be created. Our results show that the polymerase can bypass both types of lesions. Bypass occurs on a single-stranded template but is facilitated on a nicked, double-stranded template. Only purines, with guanine more favored than adenine, are incorporated across both lesions. Hesitation during bypass could not be detected. The results indicate that site-specifically modified oligonucleotides can be sensitive probes for the action of polymerases on damaged templates. They also suggest a function for polymerase I, in its nick-translation capacity, during DNA repair and mutagenesis. Images PMID:3887400

  9. Observing Comet Hyakutake from Tenerife

    NASA Astrophysics Data System (ADS)

    Mobberley, M. P.

    1996-06-01

    For the weekend of Comet Hyakutake's closest approach to Earth, photographers Martin Mobberley, Nick James and Glyn Marsh fled the clouds of England for Tenerife in the Canary Islands. Here are Martin's impressions of their experience.

  10. A multiverse play divides opinion

    NASA Astrophysics Data System (ADS)

    Crease, Robert P.

    2015-03-01

    The stage lights rise. A man and woman meet in a cute way - "Do you know why it's impossible to lick the tips of your elbows?" she asks - they chat momentarily, and separate. The play is Constellations by Nick Payne.

  11. In vitro packaging of phage DNA with defined lesions

    SciTech Connect

    Lee, E.; Hays, J.B.

    1986-05-01

    In vitro packaging of undamaged phage lambda DNA at efficiencies in excess of 2% has been routinely achieved using slightly modified standard techniques. In order to test the ability of nicks and gaps to stimulate genetic recombination during homoimmune and lytic infections, DNA was randomly nicked by treatment with methidium-propyl-EDTA-Fe(II) (MPE), and the nicks expanded to gaps with exonuclease III. DNA with about 15 MPE nicks per lambda duplex or the same number of 30-nucleotide gaps was packaged with slightly reduced efficiency. Surprisingly, DNA irradiated at 254 nm, containing 50 cyclobutane pyrimidine dimers per lambda duplex (plus other photoproducts) was packaged as efficiently as unirradiated DNA. Thus, phage containing only minor 254 nm photoproducts can be prepared efficiently by irradiation, enzymatic photoreactivation, and packaging.

  12. "Who's There?": Shakespeare and the Dragon of Autism

    ERIC Educational Resources Information Center

    Renino, Christopher

    2009-01-01

    Dan recently turned 13; Nick will soon be 12. Both boys are smart and fun; both are loving, write like angels, like to learn, and want friends; both are autistic and hope to learn to speak. They find it challenging to relate to and function in this world, and they work hard to improve their abilities to do so. Last fall, Nick and Dan became…

  13. Human DNA Ligase III Recognizes DNA Ends by Dynamic Switching between Two DNA-Bound States

    SciTech Connect

    Cotner-Gohara, Elizabeth; Kim, In-Kwon; Hammel, Michal; Tainer, John A.; Tomkinson, Alan E.; Ellenberger, Tom

    2010-09-13

    Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small-angle X-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) forms a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick sensing and the transition from nick sensing by the ZnF to nick joining by the catalytic core. The collective results support a 'jackknife model' in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers.

  14. Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states.

    PubMed

    Cotner-Gohara, Elizabeth; Kim, In-Kwon; Hammel, Michal; Tainer, John A; Tomkinson, Alan E; Ellenberger, Tom

    2010-07-27

    Human DNA ligase III has essential functions in nuclear and mitochondrial DNA replication and repair and contains a PARP-like zinc finger (ZnF) that increases the extent of DNA nick joining and intermolecular DNA ligation, yet the bases for ligase III specificity and structural variation among human ligases are not understood. Here combined crystal structure and small-angle X-ray scattering results reveal dynamic switching between two nick-binding components of ligase III: the ZnF-DNA binding domain (DBD) forms a crescent-shaped surface used for DNA end recognition which switches to a ring formed by the nucleotidyl transferase (NTase) and OB-fold (OBD) domains for catalysis. Structural and mutational analyses indicate that high flexibility and distinct DNA binding domain features in ligase III assist both nick sensing and the transition from nick sensing by the ZnF to nick joining by the catalytic core. The collective results support a "jackknife model" in which the ZnF loads ligase III onto nicked DNA and conformational changes deliver DNA into the active site. This work has implications for the biological specificity of DNA ligases and functions of PARP-like zinc fingers. PMID:20518483

  15. The Future of Theoretical Physics and Cosmology

    NASA Astrophysics Data System (ADS)

    Gibbons, G. W.; Shellard, E. P. S.; Rankin, S. J.

    2009-08-01

    Preface; List of contributors; 1. Introduction; Part I. Popular Symposium: 2. Our complex cosmos and its future Martin J. Rees; 3. Theories of everything and Hawking's wave function of the Universe James B. Hartle; 4. The problem of space-time singularities: implications for quantum gravity? Roger Penrose; 5. Warping spacetime Kip Thorne; 6. 60 years in a nutshell Stephen W. Hawking; Part II. Spacetime Singularities: 7. Cosmological perturbations and singularities George F. R. Ellis; 8. The quantum physics of chronology protection Matt Visser; 9. Energy dominance and the Hawking-Ellis vacuum conservation theorem Brandon Carter; 10. On the instability of extra space dimensions Roger Penrose; Part III. Black Holes: 11. Black hole uniqueness and the inner horizon stability problem Werner Israel; 12. Black holes in the real universe and their prospects as probes of relativistic gravity Martin J. Rees; 13. Primordial black holes Bernard Carr; 14. Black hole pair creation Simon F. Ross; 15. Black holes as accelerators Steven Giddings; Part IV. Hawking Radiation: 16. Black holes and string theory Malcolm Perry; 17. M theory and black hole quantum mechanics Joe Polchinski; 18. Playing with black strings Gary Horowitz; 19. Twenty years of debate with Stephen Leonard Susskind; Part V. Quantum Gravity: 20. Euclidean quantum gravity: the view from 2002 Gary Gibbons; 21. Zeta functions, anomalies and stable branes Ian Moss; 22. Some reflections on the status of conventional quantum theory when applied to quantum gravity Chris Isham; 23. Quantum geometry and its ramifications Abhay Ashtekar; 24. Topology change in quantum gravity Fay Dowker; Part VI. M Theory and Beyond: 25. The past and future of string theory Edward Witten; 26. String theory David Gross; 27. A brief description of string theory Michael Green; 28. The story of M Paul Townsend; 29. Gauged supergravity and holographic field theory Nick Warner; 30. 57 varieties in a NUTshell Chris Pope; Part VII. de Sitter Space

  16. Health screening - men - ages 40 to 64

    MedlinePlus

    ... pubmed/20722146 . Levin B, Lieberman DA, McFarland B, Smith RA, Brooks D, Andrews KS, et al. Screening ... cancer-screening?ds=1&s=ProstateAccessed. July 24, 2015. Smith RA, Brooks D, Cokkinides V, Saslow D, Brawley ...

  17. UNDERSTANDING PATHWAYS OF TOXICITY: MAKING SENSE OF CHANGING SIGNALS

    EPA Science Inventory

    Title:
    Understanding Pathways of Toxicity: Making sense of changing signals
    Authors & affiliations:
    Sid Hunter, Maria Blanton, Edward Karoly, Ellen Rogers, Leonard Mole, Phillip Hartig, James Andrews. Reproductive Toxicology Division, National Health and Environmental Ef...

  18. 2. VIEW OF SOUTH AND EAST SIDES OF BUILDING #2300 ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    2. VIEW OF SOUTH AND EAST SIDES OF BUILDING #2300 FACING NORTHWEST. NOTE CURVED KNEE-BRACES ON SUPPORT POSTS. - Medford Service Center, Covered Storage Building, 1319 McAndrews Road, Medford, Jackson County, OR

  19. Non-Addictive Painkiller Shows Promise in Animal Trials

    MedlinePlus

    ... the National Academy of Sciences . SOURCES: Andrew Coop, Ph.D., professor and associate dean, academic affairs, University ... School of Pharmacy, Baltimore; Stephen Waxman, M.D., Ph.D., professor of neurology, Yale University School of ...

  20. Pityriasis rubra pilaris

    MedlinePlus

    ... rubra pilaris - close-up References James WD, Berger TG, Elston DM. Pityriasis rosea, pityriasis rubra pilaris, and ... papulosquamous and hyperkeratotic diseases. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases of the Skin: ...

  1. 47. Jet Lowe, Photographer, June 1978. STAFFORD LOOM, WITH R. ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    47. Jet Lowe, Photographer, June 1978. STAFFORD LOOM, WITH R. W. ANDREWS PATENT PATTERN DEVICE, C. 1870, VIEW LOOKING FROM THE FRONT, SECOND FLOOR SOUTH. - Watkins Mill, County Highway MM, Lawson, Ray County, MO

  2. Spiders in the Crosshairs: Cobwebs, Instrument Makers, and the Search for the Perfect Line

    NASA Astrophysics Data System (ADS)

    Turner, Steven

    The the methodology of acquiring, and the use of spider webs, and other resources for creating telescope crosshairs, by early observers, such as William Herschel, David Rittenhouse, Andrew Ellicott, George Airy, as we as a variety of early manufacturers.

  3. The (nano) entrepreneur's dilemma

    NASA Astrophysics Data System (ADS)

    Maynard, Andrew D.

    2015-03-01

    Emerging technologies need to be developed responsibly if their benefits are to outweigh any potential risks. Yet do entrepreneurs really have the luxury of grappling with future consequences from the get-go, asks Andrew D. Maynard.

  4. Assessing Reading, Writing, and Reasoning.

    ERIC Educational Resources Information Center

    Sternglass, Marilyn S.

    1981-01-01

    Reports on research into the pedagogical uses of Andrew Wilkinson's writing evaluation instrument. Shows how Wilkinson's scale of cognitive development can be used both to classify writing students and to suggest strategies for helping students mature as writers. (RL)

  5. Early Geologic Education in California--Berkeley and Stanford Show the Way.

    ERIC Educational Resources Information Center

    Norris, Robert M.

    1981-01-01

    Traces the early history of geological education in California universities, with emphasis upon programs at Berkeley and Stanford. Among the pioneers in the field were Joseph LeConte, Andrew C. Lawson, and John C. Branner. (WB)

  6. 3. VIEW OF EAST SIDE AND NORTH FRONT OF BUILDING ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    3. VIEW OF EAST SIDE AND NORTH FRONT OF BUILDING #2205 (AREA CONTAINING VEHICLE REPAIR SHOP AND VEHICLE MANAGEMENT OFFICE), FACING SOUTHWEST. - Medford Service Center, Warehouse, 1319 McAndrews Road, Medford, Jackson County, OR

  7. 78 FR 12316 - Change in Bank Control Notices; Acquisitions of Shares of a Bank or Bank Holding Company

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-02-22

    ..., Minnesota 55480-0291: 1. Andrew W. Schmidt and Edward K. Massee, both of Appleton, Minnesota, as members of the Schmidt Family Group and the Massee Family Group; to acquire voting shares of MPS...

  8. Neuromyelitis Optica

    MedlinePlus

    ... directly targeted treatment of this disease. NIH Patient Recruitment for Neuromyelitis Optica Clinical Trials At NIH Clinical Center Throughout the U.S. and Worldwide NINDS Clinical Trials Organizations Column1 Column2 Multiple Sclerosis Foundation 6520 North Andrews ...

  9. Magnetotails throughout the solar system

    NASA Astrophysics Data System (ADS)

    Forsyth, C.; Milan, S. E.; Walsh, A. P.

    2010-12-01

    MEETING REPORT A key part of heliospheric and planetary physics came under scrutiny at an RAS Specialist Discussion Meeting in October. Colin Forsyth, Chris Arridge, Steve Milan and Andrew Walsh summarize.

  10. Aiming high but keeping costs low.

    PubMed

    Hough, Steve; Nicholls, Andrew

    2010-04-01

    Steve Hough, national sales manager, CEP Claddings, and Andrew Nicholls, sales director, CEP Ceilings, explain how modern cladding and ceiling materials can enhance the appearance of modern hospital buildings while offering practical, cost-saving, and environmental advantages. PMID:20446486

  11. Advanced Materials and Solids Analysis Research Core (AMSARC)

    EPA Science Inventory

    The Advanced Materials and Solids Analysis Research Core (AMSARC), centered at the U.S. Environmental Protection Agency's (EPA) Andrew W. Breidenbach Environmental Research Center in Cincinnati, Ohio, is the foundation for the Agency's solids and surfaces analysis capabilities. ...

  12. NCI Director Also to Be Interim FDA Commissioner

    Cancer.gov

    Andrew von Eschenbach, M.D., director of the NCI, was asked by President Bush on Friday, September 23, 2005, to assume the additional role of interim Commissioner of the U.S. Food and Drug Administration (FDA).

  13. Daily Low-Dose Aspirin Linked to Reduced Risk of Certain Cancers

    MedlinePlus

    ... to prevent cancer," said senior researcher Dr. Andrew Chan, from Massachusetts General Hospital in Boston. "There is ... of cancer, not that it prevents the disease, Chan said. However, other studies have come to the ...

  14. Atopic dermatitis - self-care

    MedlinePlus

    ... St. Louis, MO: Elsevier Saunders; 2016:chap 5. James WD, Berger TG, Elston DM. Atopic dermatitis, eczema, and noninfectious immunodeficiency disordersIn: James WD, Berger TG, Elston DM, eds. Andrews' Diseases ...

  15. Dyshidrotic eczema

    MedlinePlus

    ... ed. Philadelphia, PA: Elsevier Saunders; 2014:chap 190. James WD, Berger TG, Elston DM. Atopic dermatitis, eczema, and noninfectious immunodeficiency disorders. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases ...

  16. Tinea versicolor

    MedlinePlus

    ... Philadelphia, PA: Elsevier Churchill Livingstone; 2009:chap 267. James WD, Berger TG, Elston DM. Diseases resulting from fungi and yeasts. In: James WD, Berger TG, Elston DM, eds. Andrews' Diseases ...

  17. DEVELOPMENTAL TOXICITY OF DI- AND TETRACHLOROETHANE AND DICHLOROPROPANE IN EMBRYO CULTURE

    EPA Science Inventory

    DEVELOPMENTAL TOXICITY OF DI- AND TETRACHLOROETHANE AND DICHLOROPROPANE IN EMBRYO CULTURE. JE Andrews, H Nichols, and ES Hunter. Reproductive Toxicology Division, NHEERL, USEPA, RTP, NC.

    Disinfection of drinking water with chlorine results in numerous chlorinated byprodu...

  18. 7. Historic American Buildings Survey From Photograph furnished by Misses ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    7. Historic American Buildings Survey From Photograph furnished by Misses Baber-Blackshear, Macon, Georgia L. D. Andrew, Photographer Dec. 1936 MARBLE MANTEL IN PARLOR (Right Front Room) - Baber House, Walnut Street, Macon, Bibb County, GA

  19. 8. Historic American Buildings Survey From photograph from Misses BaberBlackshear, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    8. Historic American Buildings Survey From photograph from Misses Baber-Blackshear, Macon, Georgia L. D. Andrew, Photographer Dec. 1936 ARCH AND STAIRHALL ON SECOND FLOOR - Baber House, Walnut Street, Macon, Bibb County, GA

  20. 1. Historic American Buildings Survey From photograph furnished by Misses ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. Historic American Buildings Survey From photograph furnished by Misses Baber-Blackshear, Macon, Georgia L. D. Andrew, Photographer Dec. 1936 FRONT ELEVATION - Baber House, Walnut Street, Macon, Bibb County, GA

  1. 76 FR 55061 - Two Proposed CERCLA Administrative Settlement Agreements for Long-Term Access at the Bountiful...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-09-06

    ... Wynkoop Street, Denver, Colorado 80202-1129. Ms. Pokorny can be reached at (303) 312-6970. Comments should... Street, Denver, Colorado 80202-1129. Telephone: (303) 312-6970. Dated: August 26, 2011. Andrew M....

  2. Arnold, Lyons: Meeting Report: Granta MIST

    NASA Astrophysics Data System (ADS)

    Arnold, Neil; Lyons, Andrew

    2005-08-01

    The magnetosphere, ionosphere and solar-terrestrial physics community gathered at Selwyn College, University of Cambridge, for three days between Tuesday 5 April and Thursday 7 April 2005. Neil Arnold and Andrew Lyons report.

  3. Cost of Insulin Rises Threefold in Just a Decade

    MedlinePlus

    ... Medicare Part D. SOURCES: Philip Clarke, professor, health economics, University of Melbourne, Australia; Andrew Purcell, vice president and head , diabetes business unit, Sanofi, Bridgewater, N.J.; April 4, 2016, ...

  4. Webb Telescope Backplane Arrives at NASA Goddard Space Flight Center

    NASA Video Gallery

    Webb Telescope's Backplane arrived at Joint Base Andrews on Monday, August 24, 2015 aboard a U.S. Air Force C-5 cargo plane. The Backplane, inside the Space Telescope Transporter for Air Road and S...

  5. 77 FR 29360 - Used Electronic Products: An Examination of U.S. Exports Submission of Questionnaire for OMB Review

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-17

    ... specific suggested revision or language changes. Copies of any comments should be provided to Andrew Martin... ). By order of the Commission. Issued: May 11, 2012. James R. Holbein, Secretary to the...

  6. 76 FR 81965 - Remanufactured Goods: An Overview of the U.S. and Global Industries, Markets, and Trade...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-12-29

    ... provided to Andrew Martin, Chief Information Officer, U.S. International Trade Commission, 500 E Street SW... ). By order of the Commission. Issued: December 22, 2011. James R. Holbein, Secretary to the...

  7. Blood Pressure Patterns May Predict Stroke Risk

    MedlinePlus

    ... at Erasmus University Medical Center in Rotterdam, the Netherlands. High blood pressure is the number one risk ... of epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands; Andrew Rogove, M.D., Ph.D., medical director, ...

  8. Multiple data sets converge on a geologic structural model for Glass Buttes, Oregon geothermal prospect, Patrick Walsh, et al, 2010 American Geophysical Union Poster Session

    DOE Data Explorer

    Ezra Zemach

    2010-01-01

    Multiple data sets converge on a geologic structural model for Glass Buttes, Oregon geothermal prospect, Patrick Walsh, Brigette Martini, Chet Lide, Darrick Boschmann, John DIlles, Andrew Meigs, 2010 Ormat Nevada, Zonge Geophysical, Oregon State University American Geophysical Union, Poster Session

  9. 77 FR 52695 - National Sea Grant Advisory Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-08-30

    ... Program Web site at http://www.seagrant.noaa.gov/leadership/advisory_board.html . DATES: The announced....seagrant.noaa.gov/leadership/advisory_board.html . Dated: August 24, 2012. Andrew Baldus, Acting...

  10. 11. VIEW NORTH OF REAR OF TOW VEHICLE. NOTE CURRENT ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. VIEW NORTH OF REAR OF TOW VEHICLE. NOTE CURRENT COLLECTORS AT TOP LEFT AND OVERHEAD TROLLEY WIRES FOR POWER TRANSMISSION TO DRIVE MOTORS. - NASA Langley Research Center, Seaplane Towing Channel, 108 Andrews Street, Hampton, Hampton, VA

  11. Could we 3D print an artificial mind?

    NASA Astrophysics Data System (ADS)

    Maynard, Andrew D.

    2014-12-01

    3D printing is allowing more complex three-dimensional structures to be manufactured than ever before. Could the convergence between this technology and nanotechnology eventually usher in a new era of artificial intelligence, asks Andrew D. Maynard.

  12. Learning from the past

    NASA Astrophysics Data System (ADS)

    Maynard, Andrew D.

    2015-06-01

    When it comes to safety, the jury's still out on which nanoparticle characteristics we should be measuring. But, as Andrew D. Maynard explains, there's a rich history dating back over a hundred years on how we measure them.

  13. Cherokee Removal and American Politics.

    ERIC Educational Resources Information Center

    Grinde, Donald.

    1987-01-01

    Presents a brief history of the Cherokee Nation, from its first contact with De Soto in 1540 through Andrew Jackson's presidency. Concludes that the Cherokee removal clearly illustrates the shallowness of Jacksonian democratic principles. (JDH)

  14. 13. VIEW NORTHNORTHEAST OF TOW TANK FROM TOW CARRIAGE. NOTE ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    13. VIEW NORTH-NORTHEAST OF TOW TANK FROM TOW CARRIAGE. NOTE FRAME CONSTRUCTION OF STEEL TUBING AND LEFT FRONT WHEEL DRIVE MOTOR. - NASA Langley Research Center, Seaplane Towing Channel, 108 Andrews Street, Hampton, Hampton, VA

  15. Benefit of joint response in a mental health crisis.

    PubMed

    2016-07-27

    Andrew Lancaster writes in Mental Health Practice about the critical role mobile crisis teams (MCTs) can play in delivering a joint response from the police and mental health services to people in a mental health crisis. PMID:27461336

  16. Astronaut Allen during extravehicular activity (EVA) training in CCT

    NASA Technical Reports Server (NTRS)

    1993-01-01

    In the JSC Space Vehicle Mockup Facility, astronaut Andrew M. Allen retrieves gear to rehearse a suit-donning exercise on the middeck. Allen's very realistic environs are provided by the shuttle crew compartment trainer (CCT).

  17. Astronauts Allen and Gemar during extravehicular activity (EVA) training in CCT

    NASA Technical Reports Server (NTRS)

    1994-01-01

    Astronauts Charles D. (Sam) Gemar, and Andrew M. Allen participate in a training exercise at JSC's Crew Compartment Trainer (CCT), located in the Space Vehicle Mockup Facility. Gemar sits inside the airlock as Allen reviews procedures for EVA.

  18. Computers, Electronic Networking and Education: Some American Experiences.

    ERIC Educational Resources Information Center

    McConnell, David

    1991-01-01

    Describes new developments in distributed educational computing at Massachusetts Institute of Technology (MIT, "Athena"), Carnegie Mellon University ("Andrew"), Brown University "Intermedia"), Electronic University Network (California), Western Behavioral Sciences Institute (California), and University of California, Irvine. Topics discussed…

  19. 76 FR 40700 - Marine Mammals; File No. 15014-01

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-07-11

    ... Part 216). The original permit (No. 15014), issued on September 2, 2010 (75 FR 55307) authorized import... Loop, Suite 400, Orlando, FL 32819 [Brad Andrews, Responsible Party] has been issued a minor...

  20. 75 FR 55307 - Marine Mammals; File No. 15014

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-09-10

    ... INFORMATION: On December 2, 2009, notice was published in the Federal Register (74 FR 63119) that a request... Center Loop, Suite 400, Orlando, FL 32819 [Brad Andrews, Responsible Party] has been issued a permit...

  1. 76 FR 70778 - Advisory Committee on Reactor Safeguards; Notice of Meeting

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ... and participation in ACRS meetings were published in the Federal Register on October 17, 2011, (75 FR...: November 8, 2011. Andrew L. Bates, Advisory Committee Management Officer. BILLING CODE 7590-01-P...

  2. 76 FR 61118 - Advisory Committee on Reactor Safeguards; Revised Meeting Notice

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-03

    ... meeting was previously published in the Federal Register on September 26, 2011 (76 FR 59449-59450). The....gov ). Dated: September 27, 2011. Andrew L. Bates, Advisory Committee Management Officer. BILLING...

  3. Duff Green, Independent Editor of a Party Press

    ERIC Educational Resources Information Center

    Ewing, Gretchen Garst

    1977-01-01

    Tells how Duff Green obtained editorial and financial control of the "United States' Telegraph"--the primary organ for promoting the 1828 election campaign of Andrew Jackson--and how he expanded the role of partisan journalism. (GW)

  4. 76 FR 73604 - Proposed Collection; Comment Request

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-29

    ..., please write to: Cadet Summer Research Program (CSRP) Director, USAF Academy, Attn: Captain Andrew Radzicki, 2354 Fairchild Dr. Suite 6L-121, USAF Academy, CO 80840, or call the CSRP director at (719)...

  5. Proposal Writing.

    ERIC Educational Resources Information Center

    Grant, Andrew; And Others

    1988-01-01

    The basics of effective proposal writing, from content to structure to length, are presented in three articles: "Knowledge Is Power" (Andrew Grant, Emily S. Berkowitz), "Write on the Money" (Lucy Knight); and "The Problem Proposal." (MLW)

  6. Detail of parachute tower showing integration with main roof form, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of parachute tower showing integration with main roof form, facing southwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  7. Interior view at top of parachute drying tower showing original ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view at top of parachute drying tower showing original steel wall-mounted ladder, facing southwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  8. Detail of southwest corner showing rear double door entry, bracketed ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Detail of southwest corner showing rear double door entry, bracketed window awnings, and decorative parapet coping, facing northeast. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  9. Interior view of main entry hall showing stairs and original ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Interior view of main entry hall showing stairs and original pipe handrails, facing north. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  10. Oblique view of south and east sides showing parachute tower, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of south and east sides showing parachute tower, facing northwest. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  11. Oblique view of north side showing bracketed window awnings, main ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    Oblique view of north side showing bracketed window awnings, main entry, and mission coping, facing southeast. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  12. General view of building 405 in context showing hangars in ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    General view of building 405 in context showing hangars in distance, facing west. - Albrook Air Force Station, Parachute & Armament Building, 200 feet north of Andrews Boulevard, Balboa, Former Panama Canal Zone, CZ

  13. 6. Historic American Buildings Survey W. N. Manning, Photographer, September ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    6. Historic American Buildings Survey W. N. Manning, Photographer, September 11, 1935 VIEW IN HALL TOWARDS EAST, SECOND FLOOR - Andrews House, South Lafayette Street (U.S. Route 431), Lafayette, Chambers County, AL

  14. Dual daughter strand incision is processive and increases the efficiency of DNA mismatch repair.

    PubMed

    Hermans, Nicolaas; Laffeber, Charlie; Cristovão, Michele; Artola-Borán, Mariela; Mardenborough, Yannicka; Ikpa, Pauline; Jaddoe, Aruna; Winterwerp, Herrie H K; Wyman, Claire; Jiricny, Josef; Kanaar, Roland; Friedhoff, Peter; Lebbink, Joyce H G

    2016-08-19

    DNA mismatch repair (MMR) is an evolutionarily-conserved process responsible for the repair of replication errors. In Escherichia coli, MMR is initiated by MutS and MutL, which activate MutH to incise transiently-hemimethylated GATC sites. MMR efficiency depends on the distribution of these GATC sites. To understand which molecular events determine repair efficiency, we quantitatively studied the effect of strand incision on unwinding and excision activity. The distance between mismatch and GATC site did not influence the strand incision rate, and an increase in the number of sites enhanced incision only to a minor extent. Two GATC sites were incised by the same activated MMR complex in a processive manner, with MutS, the closed form of MutL and MutH displaying different roles. Unwinding and strand excision were more efficient on a substrate with two nicks flanking the mismatch, as compared to substrates containing a single nick or two nicks on the same side of the mismatch. Introduction of multiple nicks by the human MutLα endonuclease also contributed to increased repair efficiency. Our data support a general model of prokaryotic and eukaryotic MMR in which, despite mechanistic differences, mismatch-activated complexes facilitate efficient repair by creating multiple daughter strand nicks. PMID:27174933

  15. Mechanism of fragility at BCL2 gene minor breakpoint cluster region during t(14;18) chromosomal translocation.

    PubMed

    Nambiar, Mridula; Raghavan, Sathees C

    2012-03-16

    The t(14;18) translocation in follicular lymphoma is one of the most common chromosomal translocations. Breaks in chromosome 18 are localized at the 3'-UTR of BCL2 gene or downstream and are mainly clustered in either the major breakpoint region or the minor breakpoint cluster region (mcr). The recombination activating gene (RAG) complex induces breaks at IgH locus of chromosome 14, whereas the mechanism of fragility at BCL2 mcr remains unclear. Here, for the first time, we show that RAGs can nick mcr; however, the mechanism is unique. Three independent nicks of equal efficiency are generated, when both Mg(2+) and Mn(2+) are present, unlike a single nick during V(D)J recombination. Further, we demonstrate that RAG binding and nicking at the mcr are independent of nonamer, whereas a CCACCTCT motif plays a critical role in its fragility, as shown by sequential mutagenesis. More importantly, we recapitulate the BCL2 mcr translocation and find that mcr can undergo synapsis with a standard recombination signal sequence within the cells, in a RAG-dependent manner. Further, mutation to the CCACCTCT motif abolishes recombination within the cells, indicating its vital role. Hence, our data suggest a novel, physiologically relevant, nonamer-independent mechanism of RAG nicking at mcr, which may be important for generation of chromosomal translocations in humans. PMID:22275374

  16. A conserved helicase processivity factor is needed for conjugation and replication of an integrative and conjugative element.

    PubMed

    Thomas, Jacob; Lee, Catherine A; Grossman, Alan D

    2013-01-01

    Integrative and conjugative elements (ICEs) are agents of horizontal gene transfer and have major roles in evolution and acquisition of new traits, including antibiotic resistances. ICEs are found integrated in a host chromosome and can excise and transfer to recipient bacteria via conjugation. Conjugation involves nicking of the ICE origin of transfer (oriT) by the ICE-encoded relaxase and transfer of the nicked single strand of ICE DNA. For ICEBs1 of Bacillus subtilis, nicking of oriT by the ICEBs1 relaxase NicK also initiates rolling circle replication. This autonomous replication of ICEBs1 is critical for stability of the excised element in growing cells. We found a conserved and previously uncharacterized ICE gene that is required for conjugation and replication of ICEBs1. Our results indicate that this gene, helP (formerly ydcP), encodes a helicase processivity factor that enables the host-encoded helicase PcrA to unwind the double-stranded ICEBs1 DNA. HelP was required for both conjugation and replication of ICEBs1, and HelP and NicK were the only ICEBs1 proteins needed for replication from ICEBs1 oriT. Using chromatin immunoprecipitation, we measured association of HelP, NicK, PcrA, and the host-encoded single-strand DNA binding protein Ssb with ICEBs1. We found that NicK was required for association of HelP and PcrA with ICEBs1 DNA. HelP was required for association of PcrA and Ssb with ICEBs1 regions distal, but not proximal, to oriT, indicating that PcrA needs HelP to progress beyond nicked oriT and unwind ICEBs1. In vitro, HelP directly stimulated the helicase activity of the PcrA homologue UvrD. Our findings demonstrate that HelP is a helicase processivity factor needed for efficient unwinding of ICEBs1 for conjugation and replication. Homologues of HelP and PcrA-type helicases are encoded on many known and putative ICEs. We propose that these factors are essential for ICE conjugation, replication, and genetic stability. PMID:23326247

  17. A Conserved Helicase Processivity Factor Is Needed for Conjugation and Replication of an Integrative and Conjugative Element

    PubMed Central

    Thomas, Jacob; Lee, Catherine A.; Grossman, Alan D.

    2013-01-01

    Integrative and conjugative elements (ICEs) are agents of horizontal gene transfer and have major roles in evolution and acquisition of new traits, including antibiotic resistances. ICEs are found integrated in a host chromosome and can excise and transfer to recipient bacteria via conjugation. Conjugation involves nicking of the ICE origin of transfer (oriT) by the ICE–encoded relaxase and transfer of the nicked single strand of ICE DNA. For ICEBs1 of Bacillus subtilis, nicking of oriT by the ICEBs1 relaxase NicK also initiates rolling circle replication. This autonomous replication of ICEBs1 is critical for stability of the excised element in growing cells. We found a conserved and previously uncharacterized ICE gene that is required for conjugation and replication of ICEBs1. Our results indicate that this gene, helP (formerly ydcP), encodes a helicase processivity factor that enables the host-encoded helicase PcrA to unwind the double-stranded ICEBs1 DNA. HelP was required for both conjugation and replication of ICEBs1, and HelP and NicK were the only ICEBs1 proteins needed for replication from ICEBs1 oriT. Using chromatin immunoprecipitation, we measured association of HelP, NicK, PcrA, and the host-encoded single-strand DNA binding protein Ssb with ICEBs1. We found that NicK was required for association of HelP and PcrA with ICEBs1 DNA. HelP was required for association of PcrA and Ssb with ICEBs1 regions distal, but not proximal, to oriT, indicating that PcrA needs HelP to progress beyond nicked oriT and unwind ICEBs1. In vitro, HelP directly stimulated the helicase activity of the PcrA homologue UvrD. Our findings demonstrate that HelP is a helicase processivity factor needed for efficient unwinding of ICEBs1 for conjugation and replication. Homologues of HelP and PcrA-type helicases are encoded on many known and putative ICEs. We propose that these factors are essential for ICE conjugation, replication, and genetic stability. PMID:23326247

  18. Officers and Council, 1998-1999

    NASA Astrophysics Data System (ADS)

    1999-10-01

    The 1998-1999 Council of the British Astronomical Association photographed on the steps of Burlington House, London, on 1999 May 26. Front row, left to right: Bob Marriott, Hazel McGee, Martin Mobberley, Nick James, Jonathan Shanklin, Ron Johnson; centre: Richard Miles, Gordon Taylor, Jacqueline Mitton, David Tucker, John Mason, Pat Barber, David Reid, Peter Hudson; back: Laurence Anslow, Guy Hurst, Lionel Mayling, Nick Hewitt, Owen Brazell, Tony Kinder, Mark Armstrong, Maurice Gavin. Photo by Hazel McGee and Linda Newton.

  19. Permeabilization of ultraviolet-irradiated chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

    SciTech Connect

    Yarosh, D.B.; Setlow, R.B.

    1981-03-01

    Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

  20. Sequence stratigraphy and depositional systems of the paleocene submarine fans in the central North Sea: The evolution of a shelf-to-basin system

    SciTech Connect

    Reinsborough, B.C.; Galloway, W.E. )

    1993-09-01

    Slope/basin depositional systems consist of combinations of facies, including slump lobes; chute, flute, and channel fills; mounded turbidite lobes; sheet turbidites; low-density turbidite sheets and fills; hemipelagic drapes; and contourite mounds. Specific facies associations are determined by the nature (point source or linear source) and caliber (volume, grain size, sand:mud) of sediment supply to the slope. The extensive well-log, seismic, and core database was used to dissect the stratal and facies architecture of the Andrew depositional system and characterize a logical evolution of the sand-rich shelf-to-basin depositional systems tract. The andrew consists of upper and lower depositional units bounded by downlap terminations and high-gamma marker beds. The lower Andrew displays three distinct sand-rich lobes, delineated by isopach, sand percent, log motif, and seismic facies maps Proximal, mounded, sand-rich units disperse into unchannelized sheet turbidites in the basin. No extensive incised submarine valleys feed this unit, which is characterized by coarsening and thickening-upward log responses and hummocky to discontinuous reflectors. The upper Andrew downlaps the lower unit and a single, linear sediments source was centered in the Witch ground graben. The dispersal pattern and internal character suggest the upper unit is a proximal slope apron, downlapping and filling interlobe bathymetric lows of the underlying unit. Sharp-based, blocky/digitate log signatures, discontinuous chaotic reflectors, and coarse-grained sediment characterize this unit. The lower Andrew represents a structurally focused, sand-rich lobe complex, without associated incised canyons. The Andrew system evolved as the delta platform expanded onto the proximal fan, resulting in a linear sediment source spilling over the slope as a fringing slope apron.

  1. Gulf of Mexico production still recovering

    SciTech Connect

    Koen, A.D.

    1992-10-12

    This paper reports that the extent of damage caused by Hurricane Andrew to Gulf of Mexico oil and gas installations continues coming into focus. A preliminary tally by Minerals Management Service offers a reasonably complete summary of gulf production and pipeline systems damage detectable at the surface. MMS requires Outer Continental Shelf operators to inspect for underwater damage all platforms, pipelines, risers, and other structures within an 85 mile corridor along the path of Andrew's eye as it churned through the gulf. OCS operators have until Oct. 16 to submit plans for the Level II surveys.

  2. Strength and fatigue of NT551 silicon nitride and NT551 diesel exhaust valves

    SciTech Connect

    Andrews, M.J.; Werezczak, A.A.; Kirkland, T.P.; Breder, K.

    2000-02-01

    The content of this report is excerpted from Mark Andrew's Ph.D. Thesis (Andrews, 1999), which was funded by a DOE/OTT High Temperature Materials Laboratory Graduate Fellowship. It involves the characterization of NT551 and valves fabricated with it. The motivations behind using silicon nitride (Si{sub 3}N{sub 4}) as an exhaust valve for a diesel engine are presented in this section. There are several economic factors that have encouraged the design and implementation of ceramic components for internal combustion (IC) engines. The reasons for selecting the diesel engine valve for this are also presented.

  3. Hurricane-damaged Gulf of Mexico pipeline repaired with cold forging

    SciTech Connect

    Lewis, G. ); DeGruy, P. ); Avery, L. )

    1993-05-03

    Damage to Texaco Pipeline Inc.'s Eugene Island Pipeline System (EIPS) in last year's Hurricane Andrew prompted a complex repair project unique for the Gulf of Mexico. Damage, suffered when the anchor of a runaway semisubmersible drilling rig crashed into the 20-in. EPIS during the height of the storm, caused the pipeline to fail under pressure within 48 hr. after start-up following the storm. The paper describes the importance of the EIPS; system safety; Andrew's damage; locating the leak; repair options; the chosen system; mechanical bonding; end connectors and ball flanges; and diving operations.

  4. The European Perspective of HRD: A Debate on Selected Issues Put Forward By the EHRD Network. Symposium at ECER (Lisbon, Portugal, September 11-14, 2002).

    ERIC Educational Resources Information Center

    Manning, Sabine, Ed.

    This document contains 13 presentations/discussion summaries and 10 supporting 'thesis' papers from a symposium on selected issues in human resources development (HRD) in Europe. The following summaries are included: "Introduction" (Sabine Manning); "Developing Flexibility in Pursuit of Competitiveness" (Nick Boreham); "Competence and Learning in…

  5. 11. Photocopy of Photograph (Courtesy of the Detroit Hockey Club, ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    11. Photocopy of Photograph (Courtesy of the Detroit Hockey Club, Detroit, Michigan). GROUNDBREAKING FOR ADDITION, JUNE 23, 1965. Left Sid Abel, Genral Manager of the Detroit Hockey Club Center - Jerome Cavanaugh, Mayor, City of Detroit Right - Nick Landis, General Manager of the Olympia Stadium - Olympia Arena, 5920 Grand River Avenue, Detroit, MI

  6. [Accessible Rural Housing.

    ERIC Educational Resources Information Center

    Baker, Nick, Ed.

    1995-01-01

    This issue of the quarterly newsletter "Rural Exchange" provides information and resources on accessible rural housing for the disabled. "Accessible Manufactured Housing Could Increase Rural Home Supply" (Nick Baker) suggests that incorporation of access features such as lever door handles and no-step entries into manufactured housing could help…

  7. Abstract: Using System Dynamics Analysis for Evaluating the Sustainability of “Complete Streets” Practices

    EPA Science Inventory

    Abstract: Using System Dynamics Analysis for Evaluating the Sustainability of “Complete Streets” Practices Primary Author: Nicholas R. Flanders 109 T.W. Alexander Drive Mail Code: E343-02 Research Triangle Park, NC 27709 919-541-3660 Flanders.nick@Epa.gov Topic categ...

  8. Copper-acrylic enamel serves as lubricant for cold drawing of refractory metals

    NASA Technical Reports Server (NTRS)

    Beane, C.; Karasek, F.

    1966-01-01

    Acrylic enamel spray containing metallic copper pigment lubricates refractory metal tubing during cold drawing operations so that the tubing surface remains free from scratches and nicks and does not seize in the die. Zirconium alloys, zirconium, tantalum alloys, niobium alloys, vanandium alloys and titanium alloys have been drawn using this lubricant.

  9. Affordances of Equality: Rancière, Emerging Media, and the New Amateur

    ERIC Educational Resources Information Center

    Thumlert, Kurt

    2015-01-01

    This article extends a recent educational engagement with the work of Jacques Rancière by linking his meditations on 19th-century worker emancipation to present cultural contexts and media forms. Taking Nick Prior's (2010) notion of the "new amateur" as point of departure, I argue that new media and attendant production contexts offer an…

  10. 77 FR 65135 - National Emission Standards for Hazardous Air Pollutants for Chemical Manufacturing Area Sources

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-25

    ... address: parsons.nick@epa.gov . SUPPLEMENTARY INFORMATION: I. Background On October 29, 2009 (74 FR 56008... EPA intended to initiate the reconsideration process. On January 30, 2012 (77 FR 4522), the EPA... Reviews A. General Requirements Under Executive Order 12866 (58 FR 51735, October 4, 1993), this action...

  11. Hearing on H.R. 670, The School Completion and Incentives Act. Hearing before the Subcommittee on Elementary, Secondary, and Vocational Education of the Committee on Education and Labor. House of Representatives, One Hundred First Congress, First Session (Miami, Florida).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House Committee on Education and Labor.

    This document presents witness testimony and prepared statements from the Congressional hearing. Statements are included from Representatives Claude Pepper, Carl Perkins, Charles Hayes, and Nick Rahall. Witnesses providing testimony include: (1) Joseph Fernandez, Superintendent of Dade County Public Schools, Florida; (2) Nan Rich, who describes…

  12. Collision of Trapped Topoisomerase 2 with Transcription and Replication: Generation and Repair of DNA Double-Strand Breaks with 5' Adducts.

    PubMed

    Yan, Hong; Tammaro, Margaret; Liao, Shuren

    2016-01-01

    Topoisomerase 2 (Top2) is an essential enzyme responsible for manipulating DNA topology during replication, transcription, chromosome organization and chromosome segregation. It acts by nicking both strands of DNA and then passes another DNA molecule through the break. The 5' end of each nick is covalently linked to the tyrosine in the active center of each of the two subunits of Top2 (Top2cc). In this configuration, the two sides of the nicked DNA are held together by the strong protein-protein interactions between the two subunits of Top2, allowing the nicks to be faithfully resealed in situ. Top2ccs are normally transient, but can be trapped by cancer drugs, such as etoposide, and subsequently processed into DSBs in cells. If not properly repaired, these DSBs would lead to genome instability and cell death. Here, I review the current understanding of the mechanisms by which DSBs are induced by etoposide, the unique features of such DSBs and how they are repaired. Implications for the improvement of cancer therapy will be discussed. PMID:27376333

  13. 75 FR 81640 - President William Jefferson Clinton Birthplace Home National Historic Site

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-12-28

    ... National Park Service President William Jefferson Clinton Birthplace Home National Historic Site AGENCY... Birthplace Home National Historic Site.'' FOR FURTHER INFORMATION CONTACT: Contact Nick Chevance, Midwest... Birthplace Home site as a National Historic Site and unit of the National Park System, to be known as...

  14. Shaving

    MedlinePlus

    ... just a trim, you have two options – an electric razor, or a standard razor. Electric razors are generally battery-operated or rechargeable, and ... t get as close a shave with an electric razor, but it also won’t cause nicks ...

  15. Meeting the Demands of the Knowledge Based Economy: Strengthening Undergraduate Science, Mathematics and Engineering Education. Hearing Before the Subcommittee on Research, Committee on Science, House of Representatives, 107th Congress, First Session (March 7, 2002).

    ERIC Educational Resources Information Center

    Congress of the U.S., Washington, DC. House.

    This hearing was held to examine the current state of mathematics, science, and engineering education at the undergraduate level by focusing on the demands of a knowledge-based economy. Contents include opening statements by Representative Nick Smith, Chairman, Subcommittee on Research, Committee in Science, U.S. House of Representatives;…

  16. Self-Esteem and Successful Interaction as Part of the Forest School Project

    ERIC Educational Resources Information Center

    Swarbrick, Nick; Eastwood, Glynnis; Tutton, Kris

    2004-01-01

    In this article Nick Swarbrick, Glynnis Eastwood and Kris Tutton highlight the importance of the outdoor environment as an educational resource, and explore the relationship between self-esteem and successful learning through the forest school project being run in Oxfordshire. It looks at the history and practice of forest school in England, and…

  17. Storage stability of flour-blasted brown rice

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Brown rice was blasted with rice flour rather than sand in a sand blaster to make microscopic nicks and cuts so that water can easily penetrate into the brown rice endosperm and cook the rice in a shorter time. The flour-blasted American Basmati brown rice, long grain brown rice, and parboiled long...

  18. 76 FR 36903 - Privacy Act of 1974; System of Records

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-06-23

    ... ``Individual's name, nick names, Social Security Number (SSN), gender, date of birth, personal cell phone..., date of birth, personal cell phone number, home telephone number, personal e-mail addresses, mailing... (December 30, 2008, 73 FR 79841). * * * * * Changes: System Identifier: Delete entry and replace with...

  19. Needs or Rights? A Challenge to the Discourse of Special Education

    ERIC Educational Resources Information Center

    Runswick-Cole, Katherine; Hodge, Nick

    2009-01-01

    It is nearly 30 years since Mary Warnock's "Report of the Committee of Enquiry into the Education of Handicapped Children and Young People" introduced the phrase "special educational needs" into the UK education system. In this article, Katherine Runswick-Cole, Research Associate at Manchester Metropolitan University, and Nick Hodge, Principal…

  20. The Theory of Distributed Practice as Related to Acquisition of Psychomotor Skills by Adolescents in a Selected Curricular Field.

    ERIC Educational Resources Information Center

    Drake, James Bob

    1981-01-01

    From results on the tensile strength and nick-break average jury evaluations test, it was concluded that with the same total practice time, different distributions of welding practice time intervals (15, 30, and 45 minutes) influence the quality of butt welds made by ninth-grade vocational agriculture students. (Author/SJL)

  1. User Studies and Systems Design: Bridging the Gap (SIGs USE, HCI).

    ERIC Educational Resources Information Center

    Robins, David

    2000-01-01

    Presents an abstract for a planned session on the need to coordinate user studies and information retrieval system design research. Topics include human-based research and system-based research; children's information use; usability and cartographic information systems; and ethnomethodology and system design. Panelists included: Nick Belkin, Dania…

  2. The Pedagogue of the Auratic Medium--Extending the Argument

    ERIC Educational Resources Information Center

    Dobson, Stephen

    2008-01-01

    Nick Peim has recently revisited the work of Walter Benjamin; specifically his famous essay on art and mechanical reproduction. In this reply, I too draw upon the inspiration of Benjamin to extend the argument to the question of experience and what might count as knowledge, both in a philosophical sense and also in terms of the curriculum. To…

  3. Collision of Trapped Topoisomerase 2 with Transcription and Replication: Generation and Repair of DNA Double-Strand Breaks with 5′ Adducts

    PubMed Central

    Yan, Hong; Tammaro, Margaret; Liao, Shuren

    2016-01-01

    Topoisomerase 2 (Top2) is an essential enzyme responsible for manipulating DNA topology during replication, transcription, chromosome organization and chromosome segregation. It acts by nicking both strands of DNA and then passes another DNA molecule through the break. The 5′ end of each nick is covalently linked to the tyrosine in the active center of each of the two subunits of Top2 (Top2cc). In this configuration, the two sides of the nicked DNA are held together by the strong protein-protein interactions between the two subunits of Top2, allowing the nicks to be faithfully resealed in situ. Top2ccs are normally transient, but can be trapped by cancer drugs, such as etoposide, and subsequently processed into DSBs in cells. If not properly repaired, these DSBs would lead to genome instability and cell death. Here, I review the current understanding of the mechanisms by which DSBs are induced by etoposide, the unique features of such DSBs and how they are repaired. Implications for the improvement of cancer therapy will be discussed. PMID:27376333

  4. Before Coffee, Facebook: New Literacy Learning for 21st Century Teachers

    ERIC Educational Resources Information Center

    Roach, Audra K.; Beck, Jessica J.

    2012-01-01

    And so a middle school language arts teacher begins her Saturday. Before coffee, Facebook. In this themed issue on professional development in an era of nick.com, the authors propose that teachers' new literacy learning is as close as their own screens. Teachers, too, live literate lives online in this new age of composition, with impulses to…

  5. Where Does Weather Come From? A Response to "Behavioral Disorders: A Postmodern Perspective".

    ERIC Educational Resources Information Center

    Edgar, Eugene

    1998-01-01

    This response to "Behavior Disorders: A Postmodern Perspective" (David Elkind) rejects the postmodern notion that there can be no common narratives and proposes three possible metanarratives that could be useful in thinking about behavioral disorders: democracy, spirituality, and the notions of Nick Hobbs. (CR)

  6. 46 CFR 160.043-4 - Construction and workmanship.

    Code of Federal Regulations, 2013 CFR

    2013-10-01

    ... 115/16 inches long overall. It shall be so designed that the cutting action turns the ragged edge down... formed to obtain a smooth cutting action. It shall have a common nail nick on one side, and the extreme... tang shall be polished. (c) Springs. Each spring shall be of a thickness corresponding to the blade...

  7. Technology

    ERIC Educational Resources Information Center

    Breakstone, Elizabeth

    2008-01-01

    This column presents an interview with Nick Baker, Reference and Web Services librarian at Williams College. Baker has produced several films for and about the library profession. In 2007, he won an InfoTubey Award for Outstanding Library YouTube Productions for his work. In this interview, Baker shares his ideas and experiences about this…

  8. Frankie, Stoney and the Last Chance Boys.

    ERIC Educational Resources Information Center

    Hanners, Chuck

    This novel for youth describes the world and experiences of a group of poor inner city junior high school students and their teacher. The students are all in a remedial classroom designed to meet their special needs and led by a dedicated teacher nick-named "Harp On". The central character Frankie describes their school year and particularly the…

  9. Seminaries on a Hill

    ERIC Educational Resources Information Center

    Pulley, John L.

    2006-01-01

    The Andover Newton Theological School and Hebrew College, which share a hilltop campus in Boston, Massachusetts, are experimenting in academic-religious cooperation that seeks to go beyond programs that simply promote dialogue among disparate groups. The collaboration is the inspiration of Reverend Nick Carter, president of the Christian school,…

  10. How to leave your job.

    PubMed

    Gurden, Dean

    2016-08-10

    'Leaving a job is never a decision you should take lightly,' says Nick Simpson, CEO of health recruitment agency MSI Group. 'Every nursing professional has things about their job they find frustrating and daily tasks they may not necessarily enjoy doing, but it's important to consider the positive aspects of your current role before you make a decision.' PMID:27507390

  11. Seed to Seed, The Secret Life of Plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is a book review of "Seed to Seed, The Secret Life of Plants", by Nicholas Harberd. The book is a diary of ruminations/introspections about plant development and about the main research topic of Nick Harberd’s laboratory. His intended audience is initially nonscientists; many of the explanation...

  12. 78 FR 34380 - Biennial Determination of the Waste Isolation Pilot Plant's Compliance With Applicable Federal...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-07

    ... Bob Perciasepe dated May 29, 2013. FOR FURTHER INFORMATION CONTACT: Nick Stone; telephone number: (214... 6, 1445 Ross Avenue, Dallas, TX 75202. SUPPLEMENTARY INFORMATION: I. General Information A. How can..., 2013. Bob Perciasepe, Acting Administrator. BILLING CODE 6560-50-P...

  13. AASA's Message Delivery Man Has Sent His Last

    ERIC Educational Resources Information Center

    Hunter, Bruce

    2012-01-01

    On Dec. 1, Nick Penning, a longtime fixture on the advocacy team at the American Association of School Administrators (AASA), retired as the association's senior legislative analyst. In his 26 years with the association, Penning played many roles relating to advocacy, but the one he relished the most was serving as the principal communicator with…

  14. Student Engagement, Ideological Contest and Elective Affinity: The Zepke Thesis Reviewed

    ERIC Educational Resources Information Center

    Trowler, Paul

    2015-01-01

    This paper takes up issues raised in two articles by Nick Zepke and portrayed here as "the Zepke thesis". This thesis argues that the literature on, interest in and practices around student engagement in higher education have an elective affinity with neo-liberal ideology. At one level this paper counters many of the assertions that…

  15. Electronic Paper Turns the Page.

    ERIC Educational Resources Information Center

    Mann, Charles C.

    2001-01-01

    Suggests that, rather than the electronic book, the technology that is most likely to transform reading and writing will be electronic paper (e-paper). Traces the evolution of e-paper from its prototype created by Xerox PARC's Nick Sheridon in 1975 to the E Ink/Lucent e-paper made from e-ink and plastic transistors. Highlights future…

  16. Status of Fundamental Physics Program

    NASA Technical Reports Server (NTRS)

    Lee, Mark C.

    2003-01-01

    Update of the Fundamental Physics Program. JEM/EF Slip. 2 years delay. Reduced budget. Community support and advocacy led by Professor Nick Bigelow. Reprogramming led by Fred O Callaghan/JPL team. LTMPF M1 mission (DYNAMX and SUMO). PARCS. Carrier re baselined on JEM/EF.

  17. Ensuring Access and Inclusion for Marginalised Children in Extended Services: Identifying the Barriers and Promoting Choice

    ERIC Educational Resources Information Center

    Frost, Nick; Elmer, Sue; Best, Lesly; Mills, Sue

    2010-01-01

    This article by Nick Frost of Leeds Metropolitan University, Sue Elmer of Leeds Trinity University, and Lesly Best and Sue Mills, who are both Independent Researchers, looks at the experience of marginalised children in extended services. The authors conducted research into access to, and inclusion in, extended services based within schools and…

  18. Studies in Teaching 2000 Research Digest. Research Projects Presented at Annual Research Forum (Winston-Salem, North Carolina, December 2000).

    ERIC Educational Resources Information Center

    McCoy, Leah P., Ed.

    This collection of papers includes: "Cheating: Ethics and Honor of High School Students" (Nick Bender); "Assessing Listening Proficiency in High School Spanish Classes" (Michelle Bennett); "Multiple Intelligences, Assessment and Achievement in Traditional High School Classes" (Kathryn Byrnes); "Who Wants To Be a Scientist? An Investigation of High…

  19. Online Credentials: A State of Wariness

    ERIC Educational Resources Information Center

    Richardson, Jayson W.

    2010-01-01

    As a first-year superintendent, Nick Polyak has yet to conduct a search for a principal to lead one of his four schools in the Illinois Valley Central School District in Chillicothe, Ill. When that opportunity does come, Polyak is quite sure he'll be skeptical of any candidate whose curriculum vitae lists courses or entire degrees completed in…

  20. PROGRAMMED CELL DEATH IN EXTRAOCULAR MUSCLE TENDON/SCLERA PRECURSORS

    EPA Science Inventory

    Abstract

    Purpose: This study was designed to examine the occurrence of natural cell death in the periocular mesenchyme of mouse embryos.

    Methods: Vital staining with LysoTracker Red and Nile blue sulphate as well as terminal nick end labeling (TUNEL) were utiliz...

  1. Healthcare reform. Is the NHS ready for US business guru's strategy?

    PubMed

    Cavendish, Will; Edwards, Nigel; Swindells, Matthew; Henke, Nicolaus; Robinson, Edna; Smith, Richard

    2006-12-01

    The central argument of the new book by renowned US academics Michael Porter and Elizabeth Olmsted Teisberg is that the US health system is broken because rather than improving quality and efficiency, it focuses on budgetary battles. HSJ gathered together six leading healthcare insiders to discuss whether his diagnosis is applicable to the NHS. Nick Edwards was there. PMID:17228622

  2. Students Tackle Academics in Practical Context at Skateboard Factory

    ERIC Educational Resources Information Center

    Pearson, George

    2012-01-01

    High school student Nick Robertson came to Oasis Skateboard Factory (OSF) from a suburban Toronto district. He was surfing the web when he spotted a reference to Oasis Skateboard Factory, an alternative program in the Toronto District School Board. He says his first reaction was "Skateboards in school? It didn't seem possible." He applied, was…

  3. Current Research in European Vocational Education and Human Resource Development. Proceedings of the Programme Presented by the Research Network on Vocational Education and Training (VETNET) at the European Conference of Educational Research (ECER) (4th, Lille, France, September 5-8, 2001).

    ERIC Educational Resources Information Center

    Manning, Sabine, Ed.; Dif, M'Hamed, Ed.

    These proceedings are comprised of 23 presentations on research in European vocational education and human resource development. Papers include "Developing Information and Communication Technology Capability in Higher Education in the United Kingdom (UK)" (Nick Boreham); "Methodological Issues in the Study of Organizational Learning, with…

  4. Gaining Perspective on Parenting Groups.

    ERIC Educational Resources Information Center

    Fenichel, Emily, Ed.

    1996-01-01

    This theme issue offers a collection of articles focusing on support groups for parents of infants and toddlers, including the following: (1) "Gaining Perspective on Parenting Groups" (Nick Carter and Cathie Harvey) which reviews the purposes, history, and essential ingredients of such groups; (2) "The MELD Experience with Parent Groups" (Joyce…

  5. Error-free and mutagenic processing of topoisomerase 1-provoked damage at genomic ribonucleotides

    PubMed Central

    Sparks, Justin L; Burgers, Peter M

    2015-01-01

    Genomic ribonucleotides incorporated during DNA replication are commonly repaired by RNase H2-dependent ribonucleotide excision repair (RER). When RNase H2 is compromised, such as in Aicardi-Goutières patients, genomic ribonucleotides either persist or are processed by DNA topoisomerase 1 (Top1) by either error-free or mutagenic repair. Here, we present a biochemical analysis of these pathways. Top1 cleavage at genomic ribonucleotides can produce ribonucleoside-2′,3′-cyclic phosphate-terminated nicks. Remarkably, this nick is rapidly reverted by Top1, thereby providing another opportunity for repair by RER. However, the 2′,3′-cyclic phosphate-terminated nick is also processed by Top1 incision, generally 2 nucleotides upstream of the nick, which produces a covalent Top1–DNA complex with a 2-nucleotide gap. We show that these covalent complexes can be processed by proteolysis, followed by removal of the phospho-peptide by Tdp1 and the 3′-phosphate by Tpp1 to mediate error-free repair. However, when the 2-nucleotide gap is associated with a dinucleotide repeat sequence, sequence slippage re-alignment followed by Top1-mediated religation can occur which results in 2-nucleotide deletion. The efficiency of deletion formation shows strong sequence-context dependence. PMID:25777529

  6. RaPAL Bulletin, Numbers 5-13, 1988-1990.

    ERIC Educational Resources Information Center

    RaPAL Bulletin, 1988

    1988-01-01

    This document consists of a 3-year compilation (9 issues) of the RaPAL (Research and Practice in Adult Literacy) Bulletin. Typical articles are: "Student Involvement in Research" (a report of a workshop by Alex Golightly, Nick Nicola, and Marilyn Stone); part of a dialogue between Paolo Freire and Ira Shor, writer/educators of Brazil and the…

  7. Petroleum industry assists hurricane relief

    SciTech Connect

    Not Available

    1992-09-14

    This paper reports that the petroleum industry is aiding victims of last month's Hurricane Andrew with cash, clothing, food, water, and other supplies. Cash contributions announced as of last week totaled more than $2.7 million for distribution in South Florida and South Louisiana. Petroleum industry employees were collecting relief items such as bottled water and diapers for distribution in those areas.

  8. CAPSAS: Computer Assisted Program for the Selection of Appropriate Statistics.

    ERIC Educational Resources Information Center

    Shermis, Mark D.; Albert, Susan L.

    A computer-assisted program has been developed for the selection of statistics or statistical techniques by both students and researchers. Based on Andrews, Klem, Davidson, O'Malley and Rodgers "A Guide for Selecting Statistical Techniques for Analyzing Social Science Data," this FORTRAN-compiled interactive computer program was assembled to: (1)…

  9. Patterns and Trends in Grade Retention Rates in the United States, 1995-2010

    ERIC Educational Resources Information Center

    Warren, John Robert; Hoffman, Emily; Andrew, Megan

    2014-01-01

    Although grade retention may be consequential for a number of important educational and socioeconomic outcomes, we know surprisingly little about the actual rate at which students are made to repeat grades. We build on Hauser, Frederick, and Andrew's 2007 measure of grade retention using data from the 1995 through 2010 Current Population…

  10. KSC-03PD-1292

    NASA Technical Reports Server (NTRS)

    2003-01-01

    Baikonur Cosmodrome, Kazakhstan -- Astronaut Edward T. Lu, NASA ISS science officer and flight engineer for Expedition Seven, and Cosmonaut Yuri I. Malenchenko, commander, were launched onboard a Soyuz rocket at 9:53 a.m. from Baikonur, Kazakhstan. Photo Credit: NASA/Scott Andrews

  11. KSC-03PD-1291

    NASA Technical Reports Server (NTRS)

    2003-01-01

    Baikonur Cosmodrome, Kazakhstan -- Astronaut Edward T. Lu, NASA ISS science officer and flight engineer for Expedition Seven, and Cosmonaut Yuri I. Malenchenko, commander, were launched onboard a Soyuz rocket at 9:53 a.m. from Baikonur, Kazakhstan. Photo Credit: NASA/Scott Andrews

  12. Common Ground. Feminist Collaboration in the Academy.

    ERIC Educational Resources Information Center

    Peck, Elizabeth G., Ed.; Mink, JoAnna Stephens, Ed.

    The fifteen articles in this anthology examine the process of collaboration as it fits into questions of gender. Articles include: "Educate, Organize, and Agitate: A Historical Overview of Feminist Collaboration in Great Britain and America, 1640-1930" (Melodie Andrews); "Beyond Feminism: An Intercultural Challenge for Transforming the Academy"…

  13. What the White "Squaws" Want from Black Hawk: Gendering the Fan-Celebrity Relationship

    ERIC Educational Resources Information Center

    Helton, Tena L.

    2010-01-01

    Americans in the East were great fans of Black Hawk, whose popularity on tour overtook that of Andrew Jackson's parallel tour of the Northeast. Undoubtedly, then, Black Hawk was a celebrity. He remained popular even in 1837, when he attended Catlin's gallery opening in New York, which included his 1832 painting of Black Hawk. Black Hawk may also…

  14. MICROARRAY DATA ANALYSIS USING MULTIPLE STATISTICAL MODELS

    EPA Science Inventory

    Microarray Data Analysis Using Multiple Statistical Models

    Wenjun Bao1, Judith E. Schmid1, Amber K. Goetz1, Ming Ouyang2, William J. Welsh2,Andrew I. Brooks3,4, ChiYi Chu3,Mitsunori Ogihara3,4, Yinhe Cheng5, David J. Dix1. 1National Health and Environmental Effects Researc...

  15. DO ACUTE PHASE PROTEINS REFLECT SEVERITY OF INFLAMMATION IN RAT MODELS OF POLLUTANT-INDUCED LUNG INJURY?

    EPA Science Inventory

    Title: DO ACUTE PHASE PROTEINS REFLECT THE SEVERITY OF INFLAMMATION IN RAT MODELS OF POLLUTANT-INDUCED LUNG INJURY?

    M. C. Schladweiler, BS 1, P. S. Gilmour, PhD 2, D. L. Andrews, BS 1, D. L. Costa, ScD 1, A. D. Ledbetter, BS 1, K. E. Pinkerton, PhD 3 and U. P. Kodavanti, ...

  16. Could Masked Conceptual Primes Increase Recollection? The Subtleties of Measuring Recollection and Familiarity in Recognition Memory

    ERIC Educational Resources Information Center

    Taylor, Jason R.; Henson, Richard N.

    2012-01-01

    We begin with a theoretical overview of the concepts of recollection and familiarity, focusing, in the spirit of this special issue, on the important contributions made by Andrew Mayes. In particular, we discuss the issue of when the generation of semantically-related information in response to a retrieval cue might be experienced as recollection…

  17. PROCEEDINGS: CONFERENCE ON CURRENT RESEARCH IN DRINKING WATER TREATMENT HELD AT CINCINNATI, OHIO, MARCH 24-26, 1987

    EPA Science Inventory

    A Conference on Current Research in Drinking Water Treatment was held at the U.S. EPA Andrew W. Breidenbach Environmental Research Center in Cincinnati, Ohio on March 24-26, 1987. The speakers at this Conference were principally researchers funded in part by the U.S. EPA (Drinkin...

  18. Smart and intelligent sensor payload project

    NASA Technical Reports Server (NTRS)

    2009-01-01

    Engineers working on the smart and intelligent sensor payload project include (l to r): Ed Conley (NASA), Mark Mitchell (Jacobs Technology), Luke Richards (NASA), Robert Drackett (Jacobs Technology), Mark Turowski (Jacobs Technology) , Richard Franzl (seated, Jacobs Technology), Greg McVay (Jacobs Technology), Brianne Guillot (Jacobs Technology), Jon Morris (Jacobs Technology), Stephen Rawls (NASA), John Schmalzel (NASA) and Andrew Bracey (NASA).

  19. New Trends in Electronic Publishing and Electronic Libraries. Publications of Essen University Library 6. Essen Symposium (Essen, West Germany, August 29-31, 1983).

    ERIC Educational Resources Information Center

    Helal, Ahmed H., Ed.; Weiss, Joachim W., Ed.

    This symposium examined issues in the planning, development, and implementation of library automation and the effects of electronic publishing. The following presentations are included in this collection: (1) "Will There Be Electronic Anarchy?" (Helen Henderson); (2) "Ergonomics in the Electronic Era" (Andrew Torok); (3) "LS/2000--Synopsis of an…

  20. Communitarian Leadership Practice Acquisition in Educational Leadership Preparation

    ERIC Educational Resources Information Center

    Baxter, Vincent; Thessin, Rebecca A.; Clayton, Jennifer

    2014-01-01

    Principals have tremendous influence on the schools they lead (Bamburg & Andrews, 1990; Marzano et al., 2005). Certain leadership behaviors impact school level factors (Cotton, 2003; Hallinger & Heck, 2010; Leithwood, Begley, & Cousins, 1990; Marzano et al., 2005; Orr, 2003). To affect high levels of student achievement, school…

  1. "They Mean Something More!" Teaching about Symbols Using Balanced Integration

    ERIC Educational Resources Information Center

    Vesperman, Dean P.; Bernens-Kinkead, Donna J.; Loudermilk, Liesl S.; Newsom, Gladys I. M.

    2012-01-01

    Since the election of 1796, buttons, slogans, and, most importantly, symbols have become a mainstay of the American election system. The log cabin symbolized the childhoods of Andrew Jackson and Abraham Lincoln; the sun represented hope on Barack Obama's 2008 presidential election posters. Many people without formal instruction in what symbols…

  2. A Pig in a Suit Is Still A Pig: A Comment on "Modifying the C Index for Use With Holland Codes of Unequal Length"

    ERIC Educational Resources Information Center

    Tinsley, Howard E. A.

    2006-01-01

    In this comment the author reviews briefly the evidence documenting the failure of Holland's (1997) hexagonal congruence model to predict meaningful vocational outcomes, considers the excuses generally offered for the failure of Holland's system, and comments briefly on Eggerth and Andrew's (2006 [this issue]) proposed modification of the C index.…

  3. An Empirical Test of the Modified C Index and SII, O*NET, and DHOC Occupational Code Classifications

    ERIC Educational Resources Information Center

    Dik, Bryan J.; Hu, Ryan S. C.; Hansen, Jo-Ida C.

    2007-01-01

    The present study investigated new approaches for assessing Holland's congruence hypothesis by (a) developing and applying four sets of decision rules for assigning Holland codes of varying lengths for purposes of computing Eggerth and Andrew's modified C index; (b) testing the modified C index computed using these four approaches against Brown…

  4. The Complicated Pig Speaks: A Reply to Gore and Brown and Tinsley

    ERIC Educational Resources Information Center

    Eggerth, Donald E.

    2006-01-01

    In this article, responses are made to comments regarding Eggerth and Andrew (2006) by Gore and Brown (2006) calling for simplification of the modified C index and by Tinsley (2006) questioning the logic of the modified C index (all articles in this issue).

  5. "[This] Book of Odd Tales/Which Transform the Brothers Grimm": Teaching Anne Sexton's "Transformations"

    ERIC Educational Resources Information Center

    Keely, Karen A.

    2008-01-01

    Folklorist Andrew Lang, in his Preface to his 1910 edited collection "The Lilac Fairy Book", celebrates the ongoing repetition and retelling of fairy tales, including both the cozy retelling by the family fireplace. In the century since Lang wrote these words, many authors have joined the ranks of Shakespeare and Homer in putting fairy tale…

  6. 77 FR 23668 - Intent To Prepare a Draft Environmental Impact Statement for a 20-Year Dredged Material...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-04-20

    ...-Year Dredged Material Management Plan for the Atlantic Intracoastal Waterway from Port Royal Sound... Port Royal Sound, South Carolina, southward to the Georgia-Florida state line. The Corps' Savannah... Harbor Act of 1937 provided for a 7- foot protected route around St. Andrew Sound, Georgia, and for a...

  7. Autism: Clinical and Research Issues.

    ERIC Educational Resources Information Center

    Accardo, Pasquale J., Ed.; Magnusen, Christy, Ed.; Capute, Arnold J., Ed.

    This text examines the characteristics that define autism: impairments in communication; abnormal social development; and clinically significant odd behaviors. Specific chapters include: (1) Neural Mechanisms in Autism (Andrew W. Zimmerman and Barry Gordon); (2) Epidemiology of Autism and Other Pervasive Developmental Disorders: Current…

  8. H.J.Res.43 — 113th Congress (2013-2014) Removing the deadline for the ratification of the equal rights amendment.

    THOMAS, 113th Congress

    Rep. Andrews, Robert E. [D-NJ-1

    2013-05-09

    03/12/2014 Ms. Speier asked unanimous consent that she be considered the first sponsor of H.J. Res. 43, removing the deadline for the ratification of the equal rights amendment, a bill originally introduced by Representative Robert Andrews of New Jersey, for the purposes of adding... (All Actions)

  9. New England Wind Forum: A Wind Powering America Project, Volume 1, Issue 3 -- October 2007 (Newsletter)

    SciTech Connect

    Grace, R. C.; Gifford, J.

    2007-10-01

    The New England Wind Forum electronic newsletter summarizes the latest news in wind energy development activity, markets, education, and policy in the New England region. It also features an interview with a key figure influencing New England's wind energy development. Volume 1, Issue 3 features an interview with Andrew Dzykewicz, Commissioner of the Rhode Island Office of Energy Resources.

  10. A Perspective on Carnegie Corporation's Program, 1983-1997.

    ERIC Educational Resources Information Center

    Hamburg, David A.

    The Carnegie Corporation's mission is to continue Andrew Carnegie's philanthropic preoccupations with promoting education and world peace. In this essay, retiring Carnegie Corporation President David A. Hamburg provides a detailed accounting of his stewardship of the foundation since 1983, when he set forth new program directions in the context of…

  11. Chronicle of Higher Education. Volume 51, Number 39, June 3, 2005

    ERIC Educational Resources Information Center

    Chronicle of Higher Education, 2005

    2005-01-01

    "Chronicle of Higher Education" presents an abundant source of news and information for college and university faculty members and administrators. This June 3, 2005 issue of "Chronicle of Higher Education" includes the following articles: (1) "The Miraculous Sighting of a Vanished Bird" (Furman, Andrew); (2) "In the Beginning Was the Word"…

  12. Building Trust, Forging Relationships

    ERIC Educational Resources Information Center

    Principal Leadership, 2011

    2011-01-01

    Scott Andrews was a former guidance counselor with no experience in school administration when he became Amityville Memorial High School's principal in 2004, but he brought a wealth of knowledge and experience in psychology--including a doctorate--to the position. He also quickly recruited Peter Hutchison, a special education teacher, to be an…

  13. Educating in Global Times: Race, Class, Gender (and Other Processes of Normalization). Graduate Student Research Conference Proceedings (Edmonton, Alberta, Canada, March 14-15, 1997.)

    ERIC Educational Resources Information Center

    Alberta Univ., Edmonton.

    The 33 papers presented at this conference are, as follows: "Child Participation in an Everyday Adult Practice" (Andrew Brent Andressen); "Education for Sustainable Development in Southern Philippines" (Caridad Bernardino); "Distance Education and Instructional Technologies: Cultural Transmission or Cultural Erosion" (Judith Blanchette); "Domestic…

  14. 1. VIEW OF EAST SIDE AND NORTH REAR OF GASANDOIL ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    1. VIEW OF EAST SIDE AND NORTH REAR OF GAS-AND-OIL HOUSE (BUILDING #2500), FACING SOUTHWEST. (NOTE: CANOPY STRUCTURE NEXT TO BUILDING FORMERLY COVERED GAS PUMPS; CANOPY BUILT IN 1930s BUT MOVED TO THIS LOCATION IN ABOUT 1970.) - Medford Service Center, Gas & Oil House, 1319 McAndrews Road, Medford, Jackson County, OR

  15. Biomarkers of asbestos-induced lung injury: the influence of fiber characteristics and exposure methodology

    EPA Science Inventory

    ATS 2013 Biomarkers of asbestos-induced lung injury: the influence of fiber characteristics and exposure methodology Urmila P Kodavanti, Debora Andrews, Mette C Schaldweiler, Jaime M Cyphert, Darol E Dodd, and Stephen H Gavett NHEERL, U.S. EPA, Research Triangle Park, NC; NIEH...

  16. 22. Photocopy of photograph (original in the Langley Research Center ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    22. Photocopy of photograph (original in the Langley Research Center Archives, Hampton, VA LaRC) (L6415) STUFFED SEAGULL ON CARRIAGE OF TOWING TANK - 1932; EXPERIMENT TO DETERMINE AERODYNAMIC QUALITIES OF BIRDS. - NASA Langley Research Center, Seaplane Towing Channel, 108 Andrews Street, Hampton, Hampton, VA

  17. Problem-Based Labs and Group Projects in an Introductory University Physics Course

    ERIC Educational Resources Information Center

    Kohnle, Antje; Brown, C. Tom A.; Rae, Cameron F.; Sinclair, Bruce D.

    2012-01-01

    This article describes problem-based labs and analytical and computational project work we have been running at the University of St Andrews in an introductory physics course since 2008/2009. We have found the choice of topics, scaffolding of the process, timing in the year and facilitator guidance decisive for the success of these activities.…

  18. Reformers or Roadblocks: Educational Interest Groups and State Policy Formation

    ERIC Educational Resources Information Center

    Emerson, Joseph; Lemasters, Linda; Howerton, Everett

    2008-01-01

    Given the overt political nature of this topic, an additional theoretical postulate, the Triadic Theory of Power was also presented as another framework to conceptualize the external and internal forces which shape the formation of contemporary education policy. Predicated upon the scholarship of Nobel laureate James Q. Wilson, Andrew McFarland…

  19. Colleges Should Teach Students How to Borrow Wisely

    ERIC Educational Resources Information Center

    Holtschneider, Dennis H.

    2008-01-01

    The student-loan industry seems to have broken new ground, which raises a red flag after the recent investigation of the private-loan industry by Andrew M. Cuomo, New York State's attorney general. Last June "The Chronicle" reported that in Cuomo's testimony before Congress, he compared the industry to the Wild West, characterizing it as a bastion…

  20. The Turn of the Century. Tenth Grade Lesson. Schools of California Online Resources for Education (SCORE): Connecting California's Classrooms to the World.

    ERIC Educational Resources Information Center

    Bartels, Dede

    In this 10th grade social studies and language arts interdisciplinary unit, students research and report on historical figures from the turn of the 20th century. Students are required to work in pairs to learn about famous and common individuals, including Andrew Carnegie, Samuel Gompers, Susan B. Anthony, Thomas Edison, Theodore Roosevelt, Booker…

  1. Classroom Notes Plus: A Quarterly of Teaching Ideas, 2000-2001.

    ERIC Educational Resources Information Center

    Classroom Notes Plus, 2001

    2001-01-01

    This 18th volume of "Classroom Notes Plus" contains descriptions of original, unpublished teaching practices, or adapted ideas. Under the Ideas from the Classroom section, the August 2000 issue contains the following materials: "The Thought Pot" (Andrew R. West); "Seeing Is Reading: 'The Hollow Men'" (James Penha); "Language Lessons for Critical…

  2. Libraries and Electronic Publishing: Promises and Challenges for the 90's. Festschrift in Honor of Richard M. Dougherty. Proceedings of the International Essen Symposium (14th, Essen, Germany, October 14-17, 1991). Publications of Essen University Library, 14.

    ERIC Educational Resources Information Center

    Helal, Ahmed H., Ed.; Weiss, Joachim W., Ed.

    The goal of the Essen symposium was to bring together internationally recognized librarians and library automation specialists to discuss new developments in electronic publishing. All 16 papers included in this collection were presented at the conference: (1) "Barriers to the Introduction of New Technology" (J. Andrew Braid); (2) "Nudging a…

  3. Educational Rebels in the Nineteen Thirties.

    ERIC Educational Resources Information Center

    Buchanan, William

    1985-01-01

    Contrasts the unconventional educational philosophies implemented by Joseph Brewer at Olivet College (MI), John Andrews Rice at Black Mountain College (NC), and Stringfellow Barr at St. John's College (MD). Discusses the educators' personal characteristics, educational methods, and lasting influences. Recounts firsthand experiences as a student at…

  4. A NEW HIGH RESOLUTION MASS SPECTROMETRY TECHNIQUE FOR IDENTIFYING PHARMACEUTICALS AND POTENTIAL ENDOCRINE DISRUPTORS IN DRINKING WATER SOURCES

    EPA Science Inventory

    A New High Resolution Mass Spectrometry Technique for Identifying Pharmaceuticals and Potential Endocrine Disruptors in Drinking Water Sources

    Andrew H. Grange and G. Wayne Sovocool U.S.EPA, ORD, NERL, ESD, ECB, P.O. Box 93478, Las Vegas, NV 891933478

    Mass spectra...

  5. Two H's and an O: A Teaching Resource Packet on Water Education.

    ERIC Educational Resources Information Center

    Heimlich, Joe E.; And Others

    This compilation of 57 activities adapted from 32 different publications addresses 5 of the 18 needs and recommendations for water quality curricula made by Elaine Andrews in the publication "Assessing National Water Quality Education Needs for the Nonformal Youth Audience." The needs include: approaching water-related ecosystems in a more…

  6. Assessing the Future Landscape of Scholarly Communication: An Exploration of Faculty Values and Needs in Seven Disciplines. Final Report

    ERIC Educational Resources Information Center

    Harley, Diane; Acord, Sophia Krzys; Earl-Novell, Sarah; Lawrence, Shannon; King, C. Judson

    2010-01-01

    Since 2005, the Center for Studies in Higher Education (CSHE), with generous funding from the Andrew W. Mellon Foundation, has been conducting research to understand the needs and practices of faculty for in-progress scholarly communication (i.e., forms of communication employed as research is being executed) as well as archival publication. This…

  7. The Influence of Relational Complexity and Strategy Selection on Children's Reasoning in the Latin Square Task

    ERIC Educational Resources Information Center

    Perret, Patrick; Bailleux, Christine; Dauvier, Bruno

    2011-01-01

    The present study focused on children's deductive reasoning when performing the Latin Square Task, an experimental task designed to explore the influence of relational complexity. Building on Birney, Halford, and Andrew's (2006) research, we created a version of the task that minimized nonrelational factors and introduced new categories of items.…

  8. The Grief Account: Dimensions of a Contemporary Bereavement Genre

    ERIC Educational Resources Information Center

    Dennis, Michael Robert

    2008-01-01

    The genre of the grief account is identified to include published narratives of surviving grief. Thematic analysis of Andrew Holleran's (2006) "Grief: A Novel," Lolly Winston's (2004) "Good Grief: A Novel," Joan Didion's (2005) "The Year of Magical Thinking," and J. Canfield and M. V. Hansen's (2003) "Chicken Soup for the Grieving Soul: Stories…

  9. An Evaluation of the High School Redirection Program. State Urban Education Program.

    ERIC Educational Resources Information Center

    New York Univ., NY. Center for Field Research and School Services.

    The High School Redirection Program was designed to maintain 240 potential dropouts in an educational-vocational setting while assisting them to progress toward a high school diploma. Students were admitted from 13 high schools in Brooklyn and from Andrew Jackson in Queens. They were to follow a work-study program through the summer and regular…

  10. The Impact of a History of Childhood Abuse on Life as a College Student

    ERIC Educational Resources Information Center

    Sedlacek, David; Stevenson, Stanley; Kray, Carrie; Henson, Timothy; Burrows, Chelsea; Rosenboom, Mary Nell

    2015-01-01

    Several studies demonstrate the impact that childhood abuse has on adult life (Felitti et al., 1998; Galea, 2008). This study sought to examine the impact of childhood abuse on college student life. Physical, sexual, emotional, and spiritual abuse were examined in a group of college students from Andrews University and Southern Adventist…

  11. International Communications and Information; Hearings before the Subcommittee on International Operations of the Committee on Foreign Relations United States Senate, 95th Congress, First Session, June 8-10, 1977. Washington, D.C.

    ERIC Educational Resources Information Center

    Congress of the U. S., Washington, DC. Senate Committee on Foreign Affairs.

    This volume presents the hearings on the implications of international communications and information held before the Senate Subcommittee on International Operations of the Ninety-fifth Congress. Statements taken from Otis Chandler, publisher of the "Los Angeles Times," Andrew Heiskell, chair of Time Incorporated, Glenn E. Watts, president of the…

  12. Toward a Semiotic Theory of Choice and of Learning

    ERIC Educational Resources Information Center

    Stables, Andrew; Gough, Stephen

    2006-01-01

    In this essay, Andrew Stables and Stephen Gough explore some of the implications for educational policy and practice of a view of living (and, therefore, of learning) as semiotic engagement. Such a view, Stables and Gough argue, has the potential to displace or circumvent essentially Cartesian models currently dominant within learning theory…

  13. Learning To Set the Stage.

    ERIC Educational Resources Information Center

    Sommers, Rhoda C.

    Andrews University, Michigan, developed the First Day of School Experience for preservice teachers, which coincides with the opening days of school in area K-12 public and private schools where preservice teachers are placed for their student teaching experiences. The program allows preservice teachers to develop competence in beginning the school…

  14. DEVELOPMENTAL ATRAZINE EXPOSURE SUPPRESSES IMMUNE FUNCTION IN MALE, BUT NOT FEMALE SPRAGUE-DAWLEY RATS

    EPA Science Inventory

    Developmental Atrazine Exposure Suppresses Immune Function in Male, but not Female Sprague-Dawley Rats

    Andrew A. Rooney,*,1 Raymond A. Matulka,? and Robert Luebke?

    *College of Veterinary Medicine, Anatomy, Physiological Sciences and Radiology, NCSU, Raleigh, North...

  15. 76 FR 70711 - Senior Executive Service; Performance Review Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-11-15

    ..., KAREN C HALE, ANDREW M HALLMAN, TIMOTHY J HANDWERKER, ALAN I HANNIGAN, JAMES J HARDWICK JR, RAYMOND J... HARTMAN, JOHN R HARVEY, STEPHEN J HASS, RICKEY R HELD, EDWARD B HENDERSON III, CLYDE H HENNEBERGER, KAREN..., BARBARA N MCKENZIE, JOHN M MCRAE, JAMES BENNETT MEACHAM, A AVON MEEKS, TIMOTHY J MELLINGTON, STEPHEN...

  16. 75 FR 69061 - Senior Executive Service; Performance Review Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-10

    ... Hale, Andrew M Hallman, Timothy J Handschy, Mark A Handwerker, Alan I Hannigan, James J Hardwick Jr, Raymond J Harms, Timothy C Harrell, Jeffrey P Harrington, Paul G Harris, Robert J Harvey, Stephen J Held... Avon Meeks, Timothy J Mellington, Stephen A Mellington, Suzanne P Miller, Wendy L Milliken, Joann...

  17. 77 FR 64967 - Senior Executive Service; Performance Review Board

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-24

    ..., KAREN C HALE, ANDREW M HALE, JOHN H HALLMAN, TIMOTHY J HANDWERKER, ALAN I HANLON, PETER H HANNIGAN... HARRIS, ROBERT J HARROD, WILLIAM J HARVEY, STEPHEN J HELD, EDWARD B HENDERSON III, CLYDE H HENNEBERGER..., LINDA S KIRCHHOFF, STEPHEN A KLARA, SCOTT M KLAUSING, KATHLEEN A KLING, JON NMN KNOELL, THOMAS C...

  18. Protein Laboratories in Single Location | Poster

    Cancer.gov

    By Andrew Stephen, Timothy Veenstra, and Gordon Whiteley, Guest Writers, and Ken Michaels, Staff Writer The Laboratory of Proteomics and Analytical Technologies (LPAT), Antibody Characterization Laboratory (ACL), and Protein Chemistry Laboratory (PCL), previously located on different floors or in different buildings, are now together on the first floor of C wing in the ATRF.

  19. 75 FR 61133 - Marine Mammals and Endangered Species; File Nos. 808-1735, 14233, 14506, 14603, and 14726

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-10-04

    .... SUPPLEMENTARY INFORMATION: On March 11, 2010, notice was published in the Federal Register (75 FR 11516) that a... submitted by Andrew Read, Ph.D. . On September 10, 2009, notice was published in the Federal Register (74 FR... Federal Register (74 FR 66618) that a request for a permit to conduct research on green (Chelonia...

  20. Uplifting Leadership

    ERIC Educational Resources Information Center

    Hargreaves, Andy; Boyle, Alan

    2015-01-01

    To find out how organizations turn failure into success, Andrew Hargreaves and his colleagues studied more than 15 business, sports, and education organizations. They found that the secret to these organizations' success came down to just two words: uplifting leadership. Uplifting leadership, write Hargreaves and Boyle in this article, raises…

  1. 78 FR 41891 - Proposed Establishment of the Upper Hiwassee Highlands Viticultural Area

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-12

    ..., schists, metasandstones, and marble. They are generally deep, moderately to well drained, and moderately...) Unaka, NC/TN, 1957; photorevised 1978; (2) McDaniel Bald, NC/TN, 1957; photoinspected 1976; (3) Marble..., Georgia, crossing over as necessary the McDaniel Bald, Marble, Andrews, Topton, Peachtree,...

  2. Applications of the Wilkinson Model of Writing Maturity to College Writing.

    ERIC Educational Resources Information Center

    Sternglass, Marilyn

    1982-01-01

    Examines the four-category model developed by Andrew Wilkinson at the University of Essex (England) to assess growth in writing maturity. The four measures of development are stylistic, affective, cognitive, and moral. Each has several subcategories. Includes college student essays to illustrate the model. (HTH)

  3. The Principal as Instructional Leader.

    ERIC Educational Resources Information Center

    Ellis, Thomas I.

    1986-01-01

    Effective schools research has verified that schools are rarely effective unless the principal is a proficient instructional leader. This article summarizes five recent studies examining the practices and qualities comprising good instructional leadership. A Seattle study by Richard L. Andrews disclosed a statistical correlation between student…

  4. Program Design and Student Outcomes in Graduate Education

    ERIC Educational Resources Information Center

    Groen, Jeffrey A.; Jakubson, George H.; Ehrenberg, Ronald G.; Condie, Scott; Liu, Albert Y.

    2008-01-01

    Doctoral programs in the humanities and related social sciences are characterized by high attrition and long times to degree. In 1991 the Andrew W. Mellon Foundation launched the Graduate Education Initiative (GEI) to improve the quality of graduate programs and in turn reduce attrition and shorten time-to-degree. Over a 10-year period, the…

  5. The Elementary Industrial School of Cleveland, Ohio. Bulletin, 1913, No. 39. Whole Number 549

    ERIC Educational Resources Information Center

    Hailmann, W. N.

    1913-01-01

    In his report for the year 1908, Dr. Andrew S. Draper, commissioner of education of the State of New York, established the fact that current school systems still confine themselves too exclusively to preparation for professional life; that, even where they have consented to consider the claims of commerce and of certain technical pursuits, the aim…

  6. Current Challenges in Higher Education Administration and Management

    ERIC Educational Resources Information Center

    Baldwin, Jon F.

    2009-01-01

    UK higher education has a proud tradition stretching back centuries, with St. Andrews almost 600 years old, and teaching having existed at Oxford for more than 900 years. There is no reason why universities established more recently should not demonstrate similar longevity. The management of stakeholders has always been central to the successful…

  7. Beyond Walls: A Strategic Plan for James White Library.

    ERIC Educational Resources Information Center

    Andrews Univ., Berrien Springs, MI. James White Library.

    The strategic plan for the James White Library of Andrews University uses the phrase "beyond walls," rather than the catchphrase "library without walls," to acknowledge that printed matter is here to stay but that the paradigm in which it operates is open to innovation and exploration. The fundamental changes taking place in libraries will not…

  8. Midwest Research-to-Practice Annual Conference in Adult Continuing and Community Education. Conference Proceedings (11th, Manhattan, Kansas, October 8-9, 1992).

    ERIC Educational Resources Information Center

    Kansas State Univ., Manhattan. Coll. of Education.

    A conference on research to practice in adult, continuing and community education included the following papers: "Education as a Community Intervention Strategy" (Ashcraft, Andrews); "Assessing Educational Needs of Adults: An Ohio Extension Example" (Bratkovich, Miller); "Questions and Issues Related to a Lack of Multicultural Research in Adult…

  9. Facing up to Post-Retirement Medical Costs

    ERIC Educational Resources Information Center

    Biggs, John H.

    2008-01-01

    In the late 1990s, the Andrew W. Mellon Foundation made grants to study people's attitudes toward retirement and to determine what factors influenced their decisions to retire. Although faculty were not talking to college administrators or human-resources departments about health care, the researchers found to their surprise that when they…

  10. 78 FR 49510 - Cross-Media Electronic Reporting: Authorized Program Revision Approval, State of Montana

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-14

    ... the Federal Register (70 FR 59848) and codified as part 3 of title 40 of the CFR. CROMERR establishes...). Dated: August 8, 2013. Andrew Battin, Director, Office of Information Collection. BILLING CODE 6560-50-P ... State of Montana's request to revise its National Primary Drinking Water Regulations Implementation...

  11. John Wilkes Booth and the Lincoln Conspiracy.

    ERIC Educational Resources Information Center

    Nufrio, Ronald M.

    The 1865 conspiracy to assassinate Abraham Lincoln also included plans to assassinate other government officials on that same April evening. The actor, John Wilkes Booth, succeeded in killing Lincoln, but his fellow conspirators bungled their attempts to kill William Seward, Andrew Johnson, Ulysses S. Grant, and possibly Edwin Stanton. In…

  12. 77 FR 59739 - Revision to Form No. 6

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-10-01

    ..., (202) 502-6527, Andrew.Knudsen@ferc.gov . Michael Lacy (Technical Information), Office of Energy Market... throughput that combines interstate and intrastate data.\\11\\ \\8\\ Revision to Form No. 6, 76 FR 46668 (Aug. 3... and the National Propane Gas Association (ATA/ NPGA); Magellan Pipeline, L.P. (Magellan);...

  13. JSTOR: The Development of a Cost-Driven, Value-Based Pricing Model.

    ERIC Educational Resources Information Center

    Guthrie, Kevin M.

    JSTOR (Journal STORage project) began as a project of The Andrew W. Mellon Foundation designed to help libraries address growing persistent space problems. JSTOR was established as an independent not-for-profit organization with its own Board of Trustees in August 1995. This paper summarizes how JSTOR's economic model was developed, lessons…

  14. ARL: A Bimonthly Report on Research Library Issues and Actions from ARL, CNI, and SPARC. Special Issue on the Role of the Library in a Digital Landscape, Number 243

    ERIC Educational Resources Information Center

    Bowen, William G.; Barrett, G. Jaia, Ed.

    2005-01-01

    This special issue of "ARL: A Bimonthly Report" contains the remarks that William G. Bowen delivered to the research library community in October 2005 at the 147th Membership Meeting of ARL. For the past 17 years Dr. Bowen has served as President of The Andrew W. Mellon Foundation. In these remarks, entitled "New Times Always; Old Time We Cannot…

  15. "The Endless Appetite": Honors Education and the Spirit of the Humanities

    ERIC Educational Resources Information Center

    Martino, Andrew

    2015-01-01

    In a world that no longer privileges thinking, Andrew Martino writes here that we might need to consider what we are asking of our students--and why--when we ask them to think. This article presents a declaration of how Martino thinks honors education can serve as a resistant force against the increasing encroachment of a wholly utilitarian…

  16. CoSN Compendium, 2006

    ERIC Educational Resources Information Center

    Salpeter, Judy, Ed.

    2006-01-01

    The 2006 Compendium looks at six issues of vital importance to education technology leaders today: (1) Measuring the Value of One-to-One Computing (Leslie A. Wilson and Eric L. Peterson with contributions from Tammy Fry, Rich Kaestner and Andrew Zucker); (2) Open Technologies in K-12 Schools (a collaborative effort coordinated by Jim Hirsch with…

  17. Carrots, Sticks, and the Bully Pulpit: Lessons from a Half-Century of Federal Efforts to Improve America's Schools

    ERIC Educational Resources Information Center

    Hess, Frederick M., Ed.; Kelly, Andrew P., Ed.

    2012-01-01

    This timely book brings together a remarkable group of authors who examine the federal role in education policy and reform during the past fifty years. As Frederick M. Hess and Andrew P. Kelly note in their introduction, the book represents a determined effort to move beyond familiar and predictable debates and instead to focus on a number of…

  18. Leaders for a Movement: Professional Preparation and Development of Middle Level Teachers and Administrators. The Handbook of Research in Middle Level Education.

    ERIC Educational Resources Information Center

    Andrews, P. Gayle, Ed.; Anfara, Vincent A., Jr., Ed.

    Papers included in this volume include: "Leaders for Movement: An Introduction to the Professional Preparation and Development of Middle Level Teachers and Administrators" (P. Gayle Andrews and Vincent A. Anfara, Jr.); (1) "Middle Level Teacher Preparation: Status, Progress, and Challenges" (C. Kenneth McEwin, Tracy W. Smith, and Thomas S.…

  19. Cell Phones and Sun Shadows: Exploring the Equation of Time

    ERIC Educational Resources Information Center

    Madden, Sean P.

    2010-01-01

    For thousands of years before the invention of reliable clocks, humans measured their days by the motion of the sun. Astronomically, one day was the length of time it took for the sun to return to the same position in the sky. With the advent of precise mechanical chronometers such as Harrison's timekeepers (Sobel and Andrewes 1998), which ran at…

  20. Dual Coding Theory, Word Abstractness, and Emotion: A Critical Review of Kousta et al. (2011)

    ERIC Educational Resources Information Center

    Paivio, Allan

    2013-01-01

    Kousta, Vigliocco, Del Campo, Vinson, and Andrews (2011) questioned the adequacy of dual coding theory and the context availability model as explanations of representational and processing differences between concrete and abstract words. They proposed an alternative approach that focuses on the role of emotional content in the processing of…