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Sample records for ob identifikatsii izomera

  1. Walraven photometry of nearby southern OB associations

    NASA Technical Reports Server (NTRS)

    De Geus, E. J.; Lub, J.; Van De Grift, E.

    1990-01-01

    Homogeneous Walraven (VBLUW) photometry is presented for 5260 stars in the regions of five nearby southern OB associations: Scorpio Centaurus (Sco OB2), Orion OB1, Canis Major OB1, Monoceros OB1, and Scutum OB2. Derived V and (B - V) in the Johnson system are included.

  2. Sirtuin-2 Regulates Sepsis Inflammation in ob/ob Mice

    PubMed Central

    Wang, Xianfeng; Buechler, Nancy L.; Martin, Ayana; Wells, Jonathan; Yoza, Barbara; McCall, Charles E.; Vachharajani, Vidula

    2016-01-01

    Objective Obesity increases morbidity and resource utilization in sepsis patients. Sepsis transitions from early/hyper-inflammatory to late/hypo-inflammatory phase. Majority of sepsis-mortality occurs during the late sepsis; no therapies exist to treat late sepsis. In lean mice, we have shown that sirtuins (SIRTs) modulate this transition. Here, we investigated the role of sirtuins, especially the adipose-tissue abundant SIRT-2 on transition from early to late sepsis in obese with sepsis. Methods Sepsis was induced using cecal ligation and puncture (CLP) in ob/ob mice. We measured microvascular inflammation in response to lipopolysaccharide/normal saline re-stimulation as a “second-hit” (marker of immune function) at different time points to track phases of sepsis in ob/ob mice. We determined SIRT-2 expression during different phases of sepsis. We studied the effect of SIRT-2 inhibition during the hypo-inflammatory phase on immune function and 7-day survival. We used a RAW264.7 (RAW) cell model of sepsis for mechanistic studies. We confirmed key findings in diet induced obese (DIO) mice with sepsis. Results We observed that the ob/ob-septic mice showed an enhanced early inflammation and a persistent and prolonged hypo-inflammatory phase when compared to WT mice. Unlike WT mice that showed increased SIRT1 expression, we found that SIRT2 levels were increased in ob/ob mice during hypo-inflammation. SIRT-2 inhibition in ob/ob mice during the hypo-inflammatory phase of sepsis reversed the repressed microvascular inflammation in vivo via activation of endothelial cells and circulating leukocytes and significantly improved survival. We confirmed the key finding of the role of SIRT2 during hypo-inflammatory phase of sepsis in this project in DIO-sepsis mice. Mechanistically, in the sepsis cell model, SIRT-2 expression modulated inflammatory response by deacetylation of NFκBp65. Conclusion SIRT-2 regulates microvascular inflammation in obese mice with sepsis and may

  3. Ice Jams the Ob River

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Russia's Ob River flows from south to north, and each summer, it thaws in the same direction. The result is that an ice jam sits downstream from thawed portions of the river, which is laden with heavy runoff from melted snow. On June 29, 2007, the Moderate Resolution Imaging Spectroradiometer (MODIS) flying on NASA's Terra satellite captured this image of the almost completely thawed Ob River. The scene is typical for early summer. South of the ice jam, the Gulf of Ob is swollen with pent-up run-off, and upstream from that, the river is widened as well. Unable to carve through frozen land, the river has little choice but to overflow its banks. For a comparison of early summer and autumn conditions, see Flooding on the Ob River in the Earth Observatory's Natural Hazards section. Besides the annual overflow, this image captures other circumstances of early summer. Sea ice is retreating from the Kara Sea. A lingering line of snow cover snakes its way along the Ob River, to the west. And while the land is lush and green in the south, it appears barren and brown in the north. Near the mouth of the river and the Kara Sea, the land is cold-adapted tundra, with diminutive plants and a short growing season. Just as the ice plugging the river had yet to thaw in the Far North's short summer, the tundra had not yet to greened up either. In this image it still appears lifeless beige. NASA image courtesy Jeff Schmaltz, MODIS Rapid Response Team, Goddard Space Flight Center

  4. Black Rice with Giant Embryo Attenuates Obesity-Associated Metabolic Disorders in ob/ob Mice.

    PubMed

    Lee, Yoon-Mi; Han, Sank-Ik; Won, Yu-Jin; Lee, Eunji; Park, Eunju; Hwang, Seock-Yeon; Yeum, Kyung-Jin

    2016-03-30

    Obesity is closely associated with metabolic disorders such as hyperglycemia and dyslipidemia. Leptin-deficient ob/ob mice (C57BL/6J-ob/ob) and C57BL/6J mice were randomly assigned to a diet of black rice with giant embryo (BR), white rice (WR), or AIN-93G (control) and pair-fed for 14 weeks. Although there was no significant difference in body weight, BR-fed ob/ob mice had (1) significantly lower body fat mass than WR- and control-fed ob/ob mice determined by dual-energy X-ray absorptiometry; (2) significantly lower blood glucose, serum insulin, and triacylglycerol levels than control-fed ob/ob mice; and (3) significantly lower liver weight, hepatic triacylglycerol, and hepatic lipid droplets than both WR- and control-fed ob/ob mice. Furthermore, DNA damage in the liver, determined by phosphorylated H2AX protein, and in the kidney, determined by single-cell gel electrophoresis, was significantly lower in BR-fed than WR- and control-fed ob/ob mice. This study indicates that BR ameliorates obesity and its related metabolic disorders. PMID:26653400

  5. Zinc metabolism in genetically obese (ob/ob) mice

    SciTech Connect

    Kennedy, M.L.; Failla, M.L.

    1987-05-01

    Recent reports indicate that the concentrations and total amounts of several essential trace metals in various tissues of genetically obese rodents differ markedly from those in lean controls. In the present studies the absorption, retention and tissue distribution of zinc and constitutive levels of zinc-metallothionein (Zn-MT) in selected tissues were compared in obese (ob/ob) and lean (+/.) C57BL/6J mice. When 5-, 10- and 22-wk-old mice were administered 1.2 mumol /sup 65/Zn by stomach tube the apparent absorption of /sup 65/Zn by obese mice was 1.5, 2.2 and 3.9 times higher, respectively, than that in age-matched lean mice. Retention of orally administered /sup 65/Zn after 96 h was also substantially higher in obese mice than in lean mice. To assess the possible influences of hyperphagia and intestinal hypertrophy on the enhanced apparent absorption of /sup 65/Zn by obese mice food intake by an additional group of obese mice was restricted to that of age-matched lean controls. When actual absorption of zinc was determined according to the method of Heth and Hoekstra, groups of ad libitum--fed obese, pair-fed obese and lean mice absorbed 38, 32 and 18% of administered /sup 65/Zn, respectively. In contrast, the rate of /sup 65/Zn excretion 2-6 d after oral or subcutaneous administration of the metal was similar for obese and lean mice. Unrestricted and pair-fed obese mice had significantly lower percentages of carcass /sup 65/Zn present in skin, muscle plus bone, spleen and testes and higher percentages present in liver, small intestine and adipose tissue than lean mice.

  6. Telogen elongation in the hair cycle of ob/ob mice.

    PubMed

    Tasaki, Natsumi; Minematsu, Takeo; Mugita, Yuko; Ikeda, Shin-ichi; Nakagami, Gojiro; Sanada, Hiromi

    2015-01-01

    Alopecia impairs the physical and mental health of patients. We have previously shown that 8-week-old ob/ob mice have no reactivity to depilation, which is a stimulus that induces anagen transition in normal mice, while no hair cycle abnormalities have been reported in other studies until mice reach 7 weeks of age. Therefore, we hypothesized that ob/ob mice have abnormalities in hair cycle progression beyond 7 weeks of age. We examined 6- to 24-week-old ob/ob and 6- to 10-week-old normal mice. After acclimation, the dorsal skin was harvested and the hair cycle phase was identified histologically and immunohistochemically. Normal mice showed catagen-telogen and telogen-anagen transitions at 6 and 8-9 weeks old, respectively. In contrast, the anagen-catagen transition was observed in 7-week-old mice and the telogen phase was maintained from 10 to 24 weeks in most ob/ob mice. These results suggests that ob/ob mice are a possible model animal for telogen effluvium. PMID:26239026

  7. Chemical evolution of OB associations

    NASA Technical Reports Server (NTRS)

    Schramm, D. N.; Olive, K. A.

    1982-01-01

    It is determined that the existence of Al-26 and Pd-107 in meteorites in the early solar system indicates that our solar system probably formed inside an OB association that had been contaminated by the debris of at least one supernova. In addition to these radioactive tracers, the contamination of the material out of which the solar system formed would have significantly enriched the heavy element composition of the solar system relative to that of the average interstellar medium. It is found that the solar system would be enriched in those isotopes which are produced by the more massive stars, such as O-16, C-12, Ne-20, and some other r-process material. It is proposed that specific isotopic ratios and elemental ratios reflecting these differences would include the Ne-20/Ne-22 ratio, which would be higher in the solar system than in the interstellar medium and the cosmic rays, the C-12/C-13 ratio which would be higher in the solar system than in the interstellar medium, and the oxygen-to-carbon ratio, which would also be higher in the solar system than in the typical interstellar medium.

  8. Differences in early acetaminophen hepatotoxicity between obese ob/ob and db/db mice.

    PubMed

    Aubert, Jacinthe; Begriche, Karima; Delannoy, Matthieu; Morel, Isabelle; Pajaud, Julie; Ribault, Catherine; Lepage, Sylvie; McGill, Mitchell R; Lucas-Clerc, Catherine; Turlin, Bruno; Robin, Marie-Anne; Jaeschke, Hartmut; Fromenty, Bernard

    2012-09-01

    Clinical investigations suggest that hepatotoxicity after acetaminophen (APAP) overdose could be more severe in the context of obesity and nonalcoholic fatty liver disease. The pre-existence of fat accumulation and CYP2E1 induction could be major mechanisms accounting for such hepatic susceptibility. To explore this issue, experiments were performed in obese diabetic ob/ob and db/db mice. Preliminary investigations performed in male and female wild-type, ob/ob, and db/db mice showed a selective increase in hepatic CYP2E1 activity in female db/db mice. However, liver triglycerides in these animals were significantly lower compared with ob/ob mice. Next, APAP (500 mg/kg) was administered in female wild-type, ob/ob, and db/db mice, and investigations were carried out 0.5, 2, 4, and 8 h after APAP intoxication. Liver injury 8 h after APAP intoxication was higher in db/db mice, as assessed by plasma transaminases, liver histology, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. In db/db mice, however, the extent of hepatic glutathione depletion, levels of APAP-protein adducts, c-Jun N-terminal kinase activation, changes in gene expression, and mitochondrial DNA levels were not greater compared with the other genotypes. Furthermore, in the db/db genotype plasma lactate and β-hydroxybutyrate were not specifically altered, whereas the plasma levels of APAP-glucuronide were intermediary between wild-type and ob/ob mice. Thus, early APAP-induced hepatotoxicity was greater in db/db than ob/ob mice, despite less severe fatty liver and similar basal levels of transaminases. Hepatic CYP2E1 induction could have an important pathogenic role when APAP-induced liver injury occurs in the context of obesity and related metabolic disorders. PMID:22647274

  9. Serotonin transporter (SERT) and translocator protein (TSPO) expression in the obese ob/ob mouse

    PubMed Central

    2011-01-01

    Background An ever growing body of evidences is emerging concerning metabolism hormones, neurotransmitters or stress-related biomarkers as effective modulators of eating behavior and body weight in mammals. The present study sought at examining the density and affinity of two proteins related to neurotransmission and cell metabolism, the serotonin transporter SERT and the cholesterol import-benzodiazepine site TSPO (translocator protein), in a rodent leptin-lacking mutant, the obese ob/ob mouse. Binding studies were thus carried out in brain or peripheral tissues, blood platelets (SERT) and kidneys (TSPO), of ob/ob and WT mice supplied with a standard diet, using the selective radiochemical ligands [3H]-paroxetine and [3H]-PK11195. Results We observed comparable SERT number or affinity in brain and platelets of ob/ob and WT mice, whilst a significantly higher [3H]-PK11195 density was reported in the brain of ob/ob animals. TSPO binding parameters were similar in the kidneys of all tested mice. By [3H]-PK11195 autoradiography of coronal hypothalamic-hippocampal sections, an increased TSPO signal was detected in the dentate gyrus (hippocampus) and choroids plexus of ob/ob mice, without appreciable changes in the cortex or hypothalamic-thalamic regions. Conclusions These findings show that TSPO expression is up-regulated in cerebral regions of ob/ob leptin-deficient mice, suggesting a role of the translocator protein in leptin-dependent CNS trophism and metabolism. Unchanged SERT in mutant mice is discussed herein in the context of previous literature as the forerunner to a deeper biochemical investigation. PMID:21299850

  10. OBS development and data analysis in Taiwan

    NASA Astrophysics Data System (ADS)

    Lin, C.; Wang, C.; Chen, P.; Chang, H.; Jang, J.; Kuo, B.

    2012-12-01

    Since 1991 there have been various types of ocean bottom seismometers (OBS) deployed in the waters around Taiwan with different scientific goals. A few years of experiences taught us that many aspects of the instruments can be improved without too much effort. These include extension of deployment time, extension of the recording bandwidth of a short period OBS, and more flexibly to accommodate scientific demands without instrumental overhaul. Scientists will not burden themselves by these tasks, and sending back the instrument to original manufactory for modifications is usually impractical budget-wise, in timeline, and for mission goals. It is thus necessary to develop a sustainable OBS program in Taiwan. Academia Sinica has been collaborating with Taiwan Ocean Research Institute (TORI) and National Sun Yat-sen University (NSYSU) to build a new short-period OBS which has passed a series of tests and have been deployed in the Okinawa trough, Bashi channel, east Korea offshore and on service in Huatung basin. This paper describes the design goal of this OBS, component specification, and reports the preliminary analyses of data and the future directions of instrument development. Keywords: Ocean bottom seismometer, OBS

  11. Ablation of ghrelin receptor in leptin-deficient ob/ob mice has paradoxical effects on glucose homeostasis when compared with ablation of ghrelin in ob/ob mice

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The orexigenic hormone ghrelin is important in diabetes because it has an inhibitory effect on insulin secretion. Ghrelin ablation in leptin-deficient ob/ob (Ghrelin(-/-):ob/ob) mice increases insulin secretion and improves hyperglycemia. The physiologically relevant ghrelin receptor is the growth ...

  12. An anomaly of insulin removal in perfused livers of obese-hyperglycemic (ob/ob) mice.

    PubMed Central

    Karakash, C; Assimacopoulos-Jeannet, F; Jeanrenaud, B

    1976-01-01

    Obese-hyperglycemic (ob/ob) mice have the interesting feature of being hyperinsulinemic, thus having some characteristics in common with human maturity-onset diabetics. As the cause of hyperinsulinemia in these mice is not established, and as the liver is known to play a role in determining the amount of hormone that reaches the periphery, it was hypothesized that an anomaly in the hepatic handling of insulin might prevail in obese-hyperglycemic mice. Immunoreactive insulin was therefore measured in the perfusate before and after a single passage through perfused livers of lean and ob/ob mice, permitting. It was found that the removal of insulin by livers of lean mice increased with increasing concentrations of the hormone in the portal vein. The removal process had a limited capacity, however, and as a consequence the percentage of hormone removed by the liver actually decreased when portal insulin concentrations increased. Insulin removal by livers of ob/ob mice had qualitatively the same characteristics but was considerably less efficient than in normal livers. Due to this, more insulin was found in the perfusate leaving the liver of ob/9b mice than in that of controls, at any insulin concentration tested. These observations suggest that in obese-hyperglycemic mice more of the hormone may reach the periphery and thus contribute to hyperinsulinemia. The present study further suggests that the anomaly of insulin removal observed in perfused livers of ob/ob mice might be secondary to hyperinsulinemia, since it was partly corrected upon artificially decreasing the circulating levels of insulin (e.g. via a fast, anti-insulin serum, or streptozotocin treatment) before perfusion. The characteristics of hepatic insulin removal reported in this study, as well as the differences observed between livers of lean and ob/ob mice, may reflect changes in membrane insulin receptors and/or in processes responsible for the degradation of the horomone. PMID:1262459

  13. Leptin differentially regulates STAT3 activation in the ob/ob mice adipose mesenchymal stem cells

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Leptin-deficient genetically obese ob/ob mice exhibit adipocyte hypertrophy and hyperplasia as well as elevated adipose tissue and systemic inflammation. Studies have shown that multipotent stem cells isolated from adult adipose tissue can differentiate into adipocytes ex vivo and thereby contribute...

  14. Alpha-1 adrenoceptors in brown adipose tissue of lean and ob/ob mice

    SciTech Connect

    Behrens-Zaror, G.; Himms-Hagen, J.

    1986-03-01

    Obese (ob/ob) mice have a low capacity to increase thyroxine 5'-deiodinase (T4 5'-D) in brown adipose tissue (BAT) when exposed to cold. This effect is mediated by alpha-1 (A-1) adrenoceptors. The authors objective was to find out whether BAT of the ob/ob mouse has normal A-1 receptors. Saturation analysis of binding of (3H)-WB4101 at 0.05 nM to 10 ..mu..M to crude membrane preparations (100,000 g pellets from Polytron homogenates) using the LIGAND program of Munson and Rodbard, showed two populations of binding sites in BAT of lean (+/+, 11-15 wk old) mice. Acute exposure (12 h, 14/sup 0/C) or acclimation to cold (3 wk, 14/sup 0/C) did not alter affinity or concentration of sites. Displacement with yohimbine and prazosin indicated binding of WB4101 to A-1 receptors. Very young (5 wk) lean (+/.) and obese mice had similar affinity constants (lean 0.13 +/- 0.043 and 34.2 +/- 14.9; obese, 0.12 +/- 0.028 and 20.9 +/- 5.48 nM) and concentrations (lean 22.4 +/- 3.8 and 647 +/- 137; obese, 28.6 +/- 4.6 and 547 +/- 105 fmol/mg protein) of sites. Old (1 yr) mice had high affinity sites similar to those in younger animals (KD lean 0.19 +/- 0.028, obese, 0.25 +/- 0.075; Bmax lean, 60.2 +/- 12.1; obese, 63.1 +/- 13.5 fmol/mg protein). The authors conclude that the ob/ob mouse has normal high affinity A-1 receptors in BAT. Anomalous properties of low affinity binding in old ob/ob mice could not be characterized because of high nonspecific binding. BAT of the ob/ob mouse does not lack A-1 receptors but may have a post-receptor alteration in the A-1 adrenoceptor-mediated response.

  15. Adrenalectomy in genetically obese ob/ob and db/db mice increases the proton conductance pathway.

    PubMed

    Shargill, N S; Lupien, J R; Bray, G A

    1989-09-01

    Adrenalectomy (ADX) prevents the excessive weight gain in the genetically obese ob/ob and db/db mice. To test the possibility that this results from increased energy expenditure due to increased thermogenesis in brown adipose tissue (BAT), we measured GDP binding to mitochondria from interscapular brown adipose tissue (BAT) in db/db and ob/ob mice and their lean controls after adrenalectomy, with and without corticosterone replacement. Both the vehicle treated and corticosterone treated db/db and ob/ob mice had lower body weights than the sham-operated mice GDP binding to mitochondria from IBAT was significantly lower in both the db/db and ob/ob mice than in their lean controls. Adrenalectomy significantly increased GDP binding in all mice compared to the respective sham-operated mice, but, the percentage increase was always greater in the db/db and ob/ob mice. Corticosterone treatment of adrenalectomized db/db, ob/ob or lean mice lowered GDP binding to sham levels. Our data confirm previous findings that adrenalectomy results in increased GDP binding to mitochondria from IBAT. Injections of corticosterone into adrenalectomized mice results in a decrease in GDP binding to values which are similar to values in sham-operated mice. Thus adrenalectomy may inhibit the development of obesity by increasing the thermic activity in IBAT. PMID:2583669

  16. Mouth of the Ob River, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These images from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite shows the cause and effect of the large-scale seasonal flooding experienced on rivers throughout Siberia each year. Because many Siberian rivers flow from south to north, they flood regularly in the spring as meltwater from southern latitudes backs up against the still-frozen northern reaches of the rivers.These images show the Ob' River on the western edge of the Central Siberian Plateau. The images from June 20, 2002, show the mouth of the Ob' River (large river at left) where it empties into Kara Sea. In the false-color image, Vegetation appears in bright green, water appears dark blue or black, and ice appears bright blue. The ice is still choking the river's outlet to the sea.The effect of this ice block on the more southern stretches of the river can be seen in the images captured on June 17. In the false-color image, water is black, vegetation is in shades of gold and green, and clouds are pale orange. In the northernmost portion of the Ob' visible in this image (the Ob' runs southeast to northwest in the image), what is normally a fine mesh of braided streams and branches of the river channel has become almost a lake in places. The flood waters have engorged the river to 52 kilometers (32 miles) wide in places. Rivers can back up for hundreds of miles, and cause devastating flooding for towns and villages along the banks. Often, explosives are dropped into ice jams in an effort to free the river and give the flood waters a chance to escape. The spring and summer floods of 2002 have proven to be quite severe and perhaps as many as 100,000 people have been affected across the country. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  17. e-MERLIN Radio Continuum Measurements of OB Star Winds in Cyg OB2

    NASA Astrophysics Data System (ADS)

    Morford, Jack; Prinja, Raman; Fenech, Danielle Marie

    2015-08-01

    We report on the first results from the e-MERLIN Cyg OB2 Radio Survey (COBRaS), which is designed to exploit e-MERLIN's enhanced capabilities to conduct deep-field mapping of the tremendously rich Cyg OB2 association in our Galaxy. The project aims to deliver the most detailed radio census of the most massive OB association in the northern hemisphere.There is considerable evidence for structure (clumping) in the radiatively driven stellar winds of hot stars. The existence of clumping has important consequences for mass-loss rate determinations. Mass-loss rates that are not corrected for clumping provide incorrect inputs for stellar and Galactic evolution models. Radio observations are ideally suited to study the effect of clumping in the outer regions of the wind. We present the first 20 cm (L-band) continuum detections of OB stars in Cyg OB2. These data substantially increase the observational detections of the outer wind of massive stars. In combination with other observations at different wavelengths COBRaS will greatly advance our knowledge of clumping as a function of radial distance around massive stars. The observations allow us to quantify the amount of clumping and search for possible relations with stellar and/or wind parameters.

  18. A very low carbohydrate ketogenic diet improves glucose tolerance in ob/ob mice independently of weight loss.

    PubMed

    Badman, Michael K; Kennedy, Adam R; Adams, Andrew C; Pissios, Pavlos; Maratos-Flier, Eleftheria

    2009-11-01

    In mice of normal weight and with diet-induced obesity, a high-fat, low-carbohydrate ketogenic diet (KD) causes weight loss, reduced circulating glucose and lipids, and dramatic changes in hepatic gene expression. Many of the effects of KD are mediated by fibroblast growth factor 21 (FGF21). We tested the effects of KD feeding on ob/ob mice to determine if metabolic effects would occur in obesity secondarily to leptin deficiency. We evaluated the effect of prolonged KD feeding on weight, energy homeostasis, circulating metabolites, glucose homeostasis, and gene expression. Subsequently, we evaluated the effects of leptin and fasting on FGF21 expression in ob/ob mice. KD feeding of ob/ob mice normalized fasting glycemia and substantially reduced insulin and lipid levels in the absence of weight loss. KD feeding was associated with significant increases in lipid oxidative genes and reduced expression of lipid synthetic genes, including stearoyl-coenzyme A desaturase 1, but no change in expression of inflammatory markers. In chow-fed ob/ob mice, FGF21 mRNA was elevated 10-fold compared with wild-type animals, and no increase from this elevated baseline was seen with KD feeding. Administration of leptin to chow-fed ob/ob mice led to a 24-fold induction of FGF21. Fasting also induced hepatic FGF21 in ob/ob mice. Thus, KD feeding improved ob/ob mouse glucose homeostasis without weight loss or altered caloric intake. These data demonstrate that manipulation of dietary macronutrient composition can lead to marked improvements in metabolic profile of leptin-deficient obese mice in the absence of weight loss. PMID:19738035

  19. Changes in Hepatic Gene Expression upon Oral Administration of Taurine-Conjugated Ursodeoxycholic Acid in ob/ob Mice

    PubMed Central

    Jeon, Jouhyun; Park, Ho Sun; Kang, Gyeong Hoon; Park, Kyong Soo; Lee, Hong Kyu; Kim, Sanguk; Cho, Young Min

    2010-01-01

    Nonalcoholic fatty liver disease (NAFLD) is highly prevalent and associated with considerable morbidities. Unfortunately, there is no currently available drug established to treat NAFLD. It was recently reported that intraperitoneal administration of taurine-conjugated ursodeoxycholic acid (TUDCA) improved hepatic steatosis in ob/ob mice. We hereby examined the effect of oral TUDCA treatment on hepatic steatosis and associated changes in hepatic gene expression in ob/ob mice. We administered TUDCA to ob/ob mice at a dose of 500 mg/kg twice a day by gastric gavage for 3 weeks. Body weight, glucose homeostasis, endoplasmic reticulum (ER) stress, and hepatic gene expression were examined in comparison with control ob/ob mice and normal littermate C57BL/6J mice. Compared to the control ob/ob mice, TUDCA treated ob/ob mice revealed markedly reduced liver fat stained by oil red O (44.2±5.8% vs. 21.1±10.4%, P<0.05), whereas there was no difference in body weight, oral glucose tolerance, insulin sensitivity, and ER stress. Microarray analysis of hepatic gene expression demonstrated that oral TUDCA treatment mainly decreased the expression of genes involved in de novo lipogenesis among the components of lipid homeostasis. At pathway levels, oral TUDCA altered the genes regulating amino acid, carbohydrate, and drug metabolism in addition to lipid metabolism. In summary, oral TUDCA treatment decreased hepatic steatosis in ob/ob mice by cooperative regulation of multiple metabolic pathways, particularly by reducing the expression of genes known to regulate de novo lipogenesis. PMID:21079772

  20. Enhanced Hypertrophy In ob/ob Mice Due To An Impairment in Expression Of Atrial Natriuretic Peptide

    PubMed Central

    Mascareno, Eduardo; Beckles, Daniel; Dhar-Mascareno, Manya; Siddiqui, M.A.Q.

    2009-01-01

    Rationale We investigated the molecular mechanism(s) that play a role in leptin signaling during the development of left ventricular hypertrophy (LVH) due to pressure overload. To this end, ob/ob leptin deficient and C57BL/6J control mice were subjected transverse aortic constriction (TAC). Methods Control sham C57BL/6J and ob/ob mice, along with C57BL/6J and ob/ob leptin deficient mice were subjected transverse aortic constriction (TAC) for 15 days and then evaluated for morphological, physiological, and molecular changes associated with pressure overload hypertrophy. Results Evaluation by echocardiography revealed a significant increase in left ventricular mass (LVmass) and wall thickness in ob/ob mice subjected to transverse aortic constriction (TAC) as compared to C57BL/6J. Analysis of the expression of molecular markers of LVH, such as atrial natriuretic peptide (ANP), revealed a blunted increase in the level of ANP in ob/ob mice as compared to C57BL/6J mice. We observed that leptin plays a role in modulating the transcriptional activity of the promoter of the ANP gene. Leptin acts by regulating NFATc4, a member of the nuclear factor activated T cell (NFAT) family of transcription factors in cardiomyocytes. Our in vivo studies revealed that ob/ob mice subjected to TAC failed to activate the NFATc4 in the heart, however, intraperitoneal injection of leptin in ob/ob mice restored the NFATc4 DNA-binding activity and induced expression of the ANP gene. Conclusion This study establishes the role of leptin as an anti-hypertrophic agent during pressure overload hypertrophy, and suggests that a key molecular event is the leptin mediated activation of NFATc4 that regulates the transcriptional activation of the ANP gene promoter. PMID:19560554

  1. Selective T-Type Calcium Channel Blockade Alleviates Hyperalgesia in ob/ob Mice

    PubMed Central

    Latham, Janelle R.; Pathirathna, Sriyani; Jagodic, Miljen M.; Joo Choe, Won; Levin, Michaela E.; Nelson, Michael T.; Yong Lee, Woo; Krishnan, Kathiresan; Covey, Douglas F.; Todorovic, Slobodan M.; Jevtovic-Todorovic, Vesna

    2009-01-01

    OBJECTIVE Morbid obesity may be accompanied by diabetes and painful diabetic neuropathy, a poorly understood condition that is manifested by mechanical or thermal allodynia and hyperalgesia. Recent studies have highlighted the importance of T-type calcium channels (T-channels) in peripheral nociception; therefore, our goal was to examine the function of these channels in the pathophysiology and development of painful diabetic neuropathy. RESEARCH DESIGN AND METHODS In vivo testing of mechanical and thermal sensation, morphometric peripheral nerve studies, and electrophysiological and biochemical measurements were used to characterize the role of T-channels and the development of painful diabetic neuropathy in leptin-deficient (ob/ob) mice. RESULTS We found that ob/ob mice developed significant mechanical and thermal hypersensitivity early in life that coincided with hyperglycemia and was readily reversed with insulin therapy. These disturbances were accompanied by significant biophysical and biochemical modulation of T-channels in dorsal root ganglion neurons as measured by a large increase in the amplitude of T-currents and the expression of mRNA. The most prevalent subtype, α1H (Cav3.2), was most strongly affected. Moreover, (3β,5α,17β)-17-hydroxyestrane-3-carbonitrile (ECN), a novel neuroactive steroid and selective T-channel antagonist, provided dose-dependent alleviation of neuropathic thermal and mechanical hypersensitivity in diabetic ob/ob mice. CONCLUSIONS Our results indicate that pharmacological antagonism of T-channels is potentially an important novel therapeutic approach for the management of painful diabetic neuropathy. PMID:19651818

  2. Star Formation in Camelopardalis: Cam OB1

    NASA Astrophysics Data System (ADS)

    Lyder, David Anthony

    Star formation in the Cam OB1 region is investigated. Star formation, in general, is considered in terms of three elements: (a) the structural relationship between the parent molecular clouds and newly formed stars, (b) the temporal evolution of the parent molecular clouds, and (c) the probability of the occurrence of star formation. Star formation in Cam OB1, over the range in l and b considered in this work, is concentrated in the vicinity of Cam R1 and appears to have led to the formation of three distinct stellar groups: (a) Group I, formed ~1 - 50 × 10 6 yr ago, and located spatially and kinematically between two CO complexes, (b) Group II, formed ~1 - 3 × 106 yr ago, and coincident with one of the previously mentioned complexes, and (c) Group III, the youngest group, formed ~1 - 20 × 104 yr ago, and located at the current point of intersection between the two complexes in (a). The mass function (MF) for Groups I and II is similar to the cloud mass function of the parent molecular clouds, i.e., a power-law with exponent α ~ 2. A similar analysis for the Group III stars and associated molecular clouds cannot be performed due to the relatively small numbers in both samples. The star forming efficiency (SFE) in all cases is ~1%. It is proposed that cloud-cloud collisions between the CO complexes in the region triggered the formation of Groups I and III, while Group II was produced by a shock induced by the radiation pressure and stellar winds from the stars in Group I. An analysis of the molecular cloud structure in Cam OB1 and the background Perseus arm also shows that the clouds in both regions are turbulent, and typical of clouds seen elsewhere in the Galaxy. However, the clouds in Cam OB1 show a large dispersion in the degree with which they are self-gravitating, with the larger, warmer clouds being gravitationally bound. The principal data set for this work comprises fully sampled 12CO (J=1-0) observations of the western half of Cam OB1, which were

  3. Hormone-sensitive lipase deficiency suppresses insulin secretion from pancreatic islets of Lep{sup ob/ob} mice

    SciTech Connect

    Sekiya, Motohiro; Yahagi, Naoya; Tamura, Yoshiaki; Okazaki, Hiroaki; Igarashi, Masaki; Ohta, Keisuke; Takanashi, Mikio; Kumagai, Masayoshi; Takase, Satoru; Nishi, Makiko; Takeuchi, Yoshinori; Izumida, Yoshihiko; Kubota, Midori; Ohashi, Ken; Iizuka, Yoko; Yagyu, Hiroaki; Gotoda, Takanari; Nagai, Ryozo; Shimano, Hitoshi; Yamada, Nobuhiro; and others

    2009-09-25

    It has long been a matter of debate whether the hormone-sensitive lipase (HSL)-mediated lipolysis in pancreatic {beta}-cells can affect insulin secretion through the alteration of lipotoxicity. We generated mice lacking both leptin and HSL (Lep{sup ob/ob}/HSL{sup -/-}) and explored the role of HSL in pancreatic {beta}-cells in the setting of obesity. Lep{sup ob/ob}/HSL{sup -/-} developed elevated blood glucose levels and reduced plasma insulin levels compared with Lep{sup ob/ob}/HSL{sup +/+} in a fed state, while the deficiency of HSL did not affect glucose homeostasis in Lep{sup +/+} background. The deficiency of HSL exacerbated the accumulation of triglycerides in Lep{sup ob/ob} islets, leading to reduced glucose-stimulated insulin secretion. The deficiency of HSL also diminished the islet mass in Lep{sup ob/ob} mice due to decreased cell proliferation. In conclusion, HSL affects insulin secretary capacity especially in the setting of obesity.

  4. Automated identification of OB associations in M31

    NASA Technical Reports Server (NTRS)

    Magnier, Eugene A.; Battinelli, Paolo; Lewin, Walter H. G.; Haiman, Zoltan; Paradijs, Jan Van; Hasinger, Guenther; Pietsch, Wolfgang; Supper, Rodrigo; Truemper, Joachim

    1993-01-01

    A new identification of OB associations in M31 has been performed using the Path Linkage Criterion (PLC) technique of Battinelli (1991). We found 174 associations with a very small contamination (less than 5%) by random clumps of stars. The expected total number and average size of OB associations in the region of M 31 covered by our data set (Magnier et al. 1992) are approximately 280 and approximately 90 pc, respectively. M31 associations therefore have sizes similar to those of OB associations observed in nearby galaxies, so that we can consider them to be classical OB associations. This list of OB associations will be used for the study of the spatial distribution of OB associations and their correlation with other objects. Taking into account the fact that we do not cover the entire disk of M31, we extrapolate a total number of association in M31 of approximately 420.

  5. Flooding of the Ob River, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    A mixture of heavy rainfall, snowmelt, and ice jams in late May and early June of this year caused the Ob River and surrounding tributaries in Western Siberia to overflow their banks. The flooding can be seen in thess image taken on June 16, 2002, by the MODIS (Moderate Resolution Imaging Spectroradiometer) instrument aboard the Terra satellite. Last year, the river flooded farther north. Normally, the river resembles a thin black line, but floods have swollen the river considerably. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  6. New style OBS to the abyss

    NASA Astrophysics Data System (ADS)

    Shiobara, H.; Ito, A.; Sugioka, H.; Shinohara, M.

    2013-12-01

    Generally, we have been trying to widen observation ranges at the seafloor in multi dimensions, such as term of observation, period, area and strength of signal. One of them is a development of the ocean bottom seismometer (OBS) usable even at the bottom of the trench, which has been a target since 1989. Several trials encountered some troubles that would come from the mechanical design and parts used in our standard OBS. To clear this problem, we developed the New design Ultra Deep OBS (NUDOBS) and have started test observations. This OBS is a completely new concept design that can be deployed up to 10000 m depth without using any underwater cables and the traditional anchor releasing system. Its maximum observation term is about one year. The NUDOBS equips 3-component omni-directional geophones (15 Hz) with no gimbal and the 3-axis MEMS accelerometer. The 6-ch data recorder for OBS (LS9100-T6, Hakusan), 21 DD size Li-cells are installed in the titanium pressure case (Ø200×H490) with these sensors. The main point of the NUDOBS lies at the 3-stage mechanical action, the deployment stage, the observation stage and the recovery stage. The newly developed acoustic transponder that enables the two-step action controls transitions between stages twice. To reduce the noise by the bottom current, there is no mechanical coupling of the pressure case with other parts except a thin umbilical rope during the observation stage. The whole system of the NUDOBS is like as a small mooring buoy using 6 glass-sphere floats for the deep water (~12000 m). The deployment was performed to release these floats, the 20-m rope and the main unit of the NUDOBS at last. The descending speed is about 80 m/min. The NUDOBS, although it is still a prototype, has been tested twice. The first one was at the Philippine Sea site (4900 m depth) with other instruments those requires the ROV from Nov. 2012 until Feb. 2013. In this observation, we could watch two transitions of the NUDOBS by video

  7. Localization of the human OB gene (OBS) to chromosome 7q32 by fluorescence in situ hybridization

    SciTech Connect

    Geffroy, S.; Duban, B.; Martinville, B. de

    1995-08-10

    An important gene involved in the pathogenesis of obesity is the product of the human homologue of the murine obese gene (gene symbol OBS). Using fluorescence in situ hybridization (FISH), we have localized the human OB gene to human chromosome 7, specifically to region 7q32.1. The FISH data of human OBS provide a gene-associated marker for genetic mapping. 8 refs., 1 fig.

  8. Gamma-Ray Spectroscopy of Nearby OB Associations

    NASA Technical Reports Server (NTRS)

    Kaaret, Philip

    1997-01-01

    This final report is a summary of the study on gamma ray spectroscopy of nearby OB associations. The goal of this work is to investigate the gamma ray line emission detected with the Compton Telescope (COMPTEL) from the Orion star forming region. This is accomplished by searching for similar emission from other nearby OB associations.

  9. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers

  10. The origin of OB runaway stars.

    PubMed

    Fujii, Michiko S; Portegies Zwart, Simon

    2011-12-01

    About 20% of all massive stars in the Milky Way have unusually high velocities, the origin of which has puzzled astronomers for half a century. We argue that these velocities originate from strong gravitational interactions between single stars and binaries in the centers of star clusters. The ejecting binary forms naturally during the collapse of a young (≤1 million years old) star cluster. This model replicates the key characteristics of OB runaways in our galaxy, and it explains the presence of runaway stars of ≥100 solar masses (M(⊙)) around young star clusters, such as R136 and Westerlund 2. The high proportion and the distributions in mass and velocity of runaways in the Milky Way are reproduced if the majority of massive stars are born in dense and relatively low-mass (5000 to 10,000 M(⊙)) clusters. PMID:22096104

  11. Photometry of stars in the Cas OB5 Associations

    NASA Astrophysics Data System (ADS)

    Tanriver, Mehmet; Keskin, Ahmet

    2016-07-01

    OB associations are a grouping of very young associations, contain 10-100 very hot massive stars, spectral types O and B. Also, the OB associations contain low and intermediate mass stars, too. Association members are believed to form within the same small volume inside a giant molecular cloud. Once the surrounding dust and gas is blown away, the remaining stars become not tied up and begin to drift separately. It is believed that the majority of all stars in the Milky Way were formed in OB associations. O type stars are short-lived, and will be at an end as supernovae after roundly a million years. OB associations are generally only a few million years in age or less. In this study, the photometry of UU Cas and field star which been Cas OB5 association member was carried out. Light curves and color diagrams are given in the study.

  12. Open clusters in Auriga OB2

    NASA Astrophysics Data System (ADS)

    Marco, Amparo; Negueruela, Ignacio

    2016-06-01

    We study the area around the H II region Sh 2-234, including the young open cluster Stock 8, to investigate the extent and definition of the association Aur OB2 and the possible role of triggering in massive cluster formation. We obtained Strömgren and J, H, KS photometry for Stock 8 and Strömgren photometry for two other cluster candidates in the area, which we confirm as young open clusters and name Alicante 11 and Alicante 12. We took spectroscopy of ˜33 early-type stars in the area, including the brightest cluster members. We calculate a common distance of 2.80^{+0.27}_{-0.24} kpc for the three open clusters and surrounding association. We derive an age 4-6 Ma for Stock 8, and do not find a significantly different age for the other clusters or the association. The star LS V +34°23, with spectral type O8 II(f), is likely the main source of ionization of Sh 2-234. We observe an important population of pre-main-sequence stars, some of them with discs, associated with the B-type members lying on the main sequence. We interpret the region as an area of recent star formation with some residual and very localized ongoing star formation. We do not find evidence for sequential star formation on a large scale. The classical definition of Aur OB2 has to be reconsidered, because its two main open clusters, Stock 8 and NGC 1893, are not at the same distance. Stock 8 is probably located in the Perseus arm, but other nearby H II regions whose distances also place them in this arm show quite different distances and radial velocities and, therefore, are not connected.

  13. Effect of Leptin Replacement on PCSK9 in ob/ob Mice and Female Lipodystrophic Patients.

    PubMed

    Levenson, Amy E; Haas, Mary E; Miao, Ji; Brown, Rebecca J; de Ferranti, Sarah D; Muniyappa, Ranganath; Biddinger, Sudha B

    2016-04-01

    Leptin treatment has beneficial effects on plasma lipids in patients with lipodystrophy, but the underlying mechanism is unknown. Proprotein convertase subtilisin/kexin type 9 (PCSK9) decreases low-density lipoprotein (LDL) clearance, promotes hypercholesterolemia, and has recently emerged as a novel therapeutic target. To determine the effect of leptin on PCSK9, we treated male and female ob/ob mice with leptin for 4 days via sc osmotic pumps (∼24 μg/d). Leptin reduced body weight and food intake in all mice, but the effects of leptin on plasma PCSK9 and lipids differed markedly between the sexes. In male mice, leptin suppressed PCSK9 but had no effect on plasma triglycerides or cholesterol. In female mice, leptin suppressed plasma triglycerides and cholesterol but had no effect on plasma PCSK9. In parallel, we treated female lipodystrophic patients (8 females, ages 5-23 y) with sc metreleptin injections (∼4.4 mg/d) for 4-6 months. In this case, leptin reduced plasma PCSK9 by 26% (298 ± 109 vs 221 ± 102 ng/mL; n = 8; P = .008), and the change in PCSK9 was correlated with a decrease in LDL cholesterol (r(2) = 0.564, P = .03). In summary, in leptin-deficient ob/ob mice, the effects of leptin on PCSK9 and plasma lipids appeared to be independent of one another and strongly modified by sex. On the other hand, in lipodystrophic females, leptin treatment reduced plasma PCSK9 in parallel with LDL cholesterol. PMID:26824363

  14. Synchronous OBS solution to support TDM and data services in OBS ring network

    NASA Astrophysics Data System (ADS)

    Qiao, Yaojun; Kubo, Tokuro; Saito, Tamio

    2006-09-01

    We suggest a new protocol for Optical-Burst-Switched (OBS) ring network to support synchronous services (SONET/SDH, named TDM services) and asynchronous services (data services) expediently. In the new protocol, we use different bandwidth assignment methods for the two kinds of services. For synchronous services, we use fixed bandwidth assignment to broadcast the TDM slots to all remained nodes in optical domain; for asynchronous services, we use dynamic bandwidth assignment by OBS technologies. The data channel is frame based with 125μs frame period. In each 125μs frame, there are several reserved time slots for TDM services, and the remained time block can be used to transport data services. The main features of this protocol: 1) the bandwidth ratio of TDM and data services can be adjusted flexibly basing on demand; 2) the TDM services can be transported with guaranteed QoS; 3) the data services can be transported in data burst with statistic multiplexing profit; 4) we can transport TDM and data services in same data channel with OBS technology; 5) the total bandwidth utilization is about 70%.

  15. Leptin Increases Striatal Dopamine D2 Receptor Binding in Leptin-Deficient Obese (ob/ob) Mice

    SciTech Connect

    Pfaffly, J.; Michaelides, M.; Wang, G-J.; Pessin, J.E.; Volkow, N.D.; Thanos, P.K.

    2010-06-01

    Peripheral and central leptin administration have been shown to mediate central dopamine (DA) signaling. Leptin-receptor deficient rodents show decreased DA D2 receptor (D2R) binding in striatum and unique DA profiles compared to controls. Leptin-deficient mice show increased DA activity in reward-related brain regions. The objective of this study was to examine whether basal D2R-binding differences contribute to the phenotypic behaviors of leptin-deficient ob/ob mice, and whether D2R binding is altered in response to peripheral leptin treatment in these mice. Leptin decreased body weight, food intake, and plasma insulin concentration in ob/ob mice but not in wild-type mice. Basal striatal D2R binding (measured with autoradiography [{sup 3}H] spiperone) did not differ between ob/ob and wild-type mice but the response to leptin did. In wild-type mice, leptin decreased striatal D2R binding, whereas, in ob/ob mice, leptin increased D2R binding. Our findings provide further evidence that leptin modulates D2R expression in striatum and that these effects are genotype/phenotype dependent.

  16. Anti-obesity and anti-diabetic effects of a standardized potato extract in ob/ob mice

    PubMed Central

    KU, SAE KWANG; SUNG, SOO HYUN; CHOUNG, JAI JUN; CHOI, JAE-SUK; SHIN, YONG KOOK; KIM, JOO WAN

    2016-01-01

    The potato (Solanum tuberosum) has been cultivated globally for food for millenia. Potato contains proteinase inhibitor II, which catalyzes the release of cholecystokinin (CCK), leading to delayed gastric emptying in humans. The present study investigated the anti-obesity effects of Slendesta™ Potato Extract (SLD), a standardized potato extract containing 5% proteinase inhibitor II, in the ob/ob obese mice. Three doses of SLD (50, 150 or 300 mg/kg) were orally administered to ob/ob mice once a day for 28 days, whereas control mice were administered distilled water. Four weeks after SLD treatment, the changes in body weight, food consumption, epididymal fat weight, serum chemistry, insulin, leptin and adiponectin contents, and fat histopathology were determined and compared with ob/ob mice treated with 750 mg/kg conjugate linoleic acid. As a result of SLD treatment in the obese mice, body weight, food consumption, epididymal fat, serum biochemistry, histomorphological changes of fat and pancreas were significantly and dose-dependently decreased compared with ob/ob control mice. These obesity and type 2 diabetes associated alterations were significantly inhibited after SLD treatment for 28 days. Thus, the present results indicate that SLD has potential as an alternative therapeutic agent for obesity. PMID:27347062

  17. PPARα-Deficient ob/ob Obese Mice Become More Obese and Manifest Severe Hepatic Steatosis Due to Decreased Fatty Acid Oxidation

    PubMed Central

    Gao, Qian; Jia, Yuzhi; Yang, Gongshe; Zhang, Xiaohong; Boddu, Prajwal C.; Petersen, Bryon; Narsingam, Saiprasad; Zhu, Yi-Jun; Thimmapaya, Bayar; Kanwar, Yashpal S.; Reddy, Janardan K.

    2016-01-01

    Obesity poses an increased risk of developing metabolic syndrome and closely associated nonalcoholic fatty liver disease, including liver cancer. Satiety hormone leptin-deficient (ob/ob) mice, considered paradigmatic of nutritional obesity, develop hepatic steatosis but are less prone to developing liver tumors. Sustained activation of peroxisome proliferator–activated receptor α (PPARα) in ob/ob mouse liver increases fatty acid oxidation (FAO), which contributes to attenuation of obesity but enhances liver cancer risk. To further evaluate the role of PPARα-regulated hepatic FAO and energy burning in the progression of fatty liver disease, we generated PPARα-deficient ob/ob (PPARαΔob/ob) mice. These mice become strikingly more obese compared to ob/ob littermates, with increased white and brown adipose tissue content and severe hepatic steatosis. Hepatic steatosis becomes more severe in fasted PPARαΔob/ob mice as they fail to up-regulate FAO systems. PPARαΔob/ob mice also do not respond to peroxisome proliferative and mitogenic effects of PPARα agonist Wy-14,643. Although PPARαΔob/ob mice are severely obese, there was no significant increase in liver tumor incidence, even when maintained on a diet containing Wy-14,643. We conclude that sustained PPARα activation–related increase in FAO in fatty livers of obese ob/ob mice increases liver cancer risk, whereas deletion of PPARα in ob/ob mice aggravates obesity and hepatic steatosis. However, it does not lead to liver tumor development because of reduction in FAO and energy burning. PMID:25773177

  18. Additional red and reddened stars in Cyg OB2 association

    NASA Technical Reports Server (NTRS)

    Parthasarathy, M.; Jain, S. K.

    1989-01-01

    Several new red and reddened stars are detected in the most heavily reddened associations Cyg OB2. About 47 IRAS sources are detected in Cyg OB2. Their flux distributions, and colors, suggest that they are young stellar objects embedded in dust envelopes or disks (some of them may be proto stars) and are most likely members of the Cyg OB2 association. The large values of the flux ratio L sub IR/L sub VIS suggests that the central objects are obscured because of very large extinction.

  19. New OBS network deployment offshore Ireland

    NASA Astrophysics Data System (ADS)

    Le Pape, Florian; Bean, Chris; Craig, David; Jousset, Philippe; Horan, Clare; Hogg, Colin; Donne, Sarah; McCann, Hannah; Möllhoff, Martin; Kirk, Henning; Ploetz, Aline

    2016-04-01

    With the presence of the stormy NE Atlantic, Ireland is ideally located to investigate further our understanding of ocean generated microseisms and use noise correlation methods to develop seismic imaging in marine environments as well as time-lapse monitoring. In order to study the microseismic activity offshore Ireland, 10 Broad Band Ocean Bottom Seismographs (OBSs) units including hydrophones have been deployed in January 2016 across the shelf offshore Donegal and out into the Rockall Trough. This survey represents the first Broadband passive study in this part of the NE Atlantic. The instruments will be recovered in August 2016 providing 8 months worth of data to study microseisms but also the offshore seismic activity in the area. One of the main goal of the survey is to investigate the spatial and temporal distributions of dominant microseism source regions, close to the microseism sources. Additionally we will study the coupling of seismic and acoustic signals at the sea bed and its evolution in both the deep water and continental shelf areas. Furthermore, the survey also aims to investigate further the relationship between sea state conditions (e.g. wave height, period), seafloor pressure variations and seismic data recorded on both land and seafloor. Finally, the deployed OBS network is also the first ever attempt to closely monitor local offshore earthquakes in Ireland. Ireland seismicity although relatively low can reduce slope stability and poses the possibility of triggering large offshore landslides and local tsunamis.

  20. Optical Spectroscopic Confirmation of OB Star Candidates in HII Regions

    NASA Astrophysics Data System (ADS)

    Tsujimoto, Masahiro; Kobayashi, Naoto

    2007-08-01

    The number of OB stars is the most basic quantity to characterize massive star clusters. We studied several massive clusters using Chandra X-ray observatory and found 30--80 new OB star candidates in RCW49, NGC3603, and NGC3576. The numbers are about 2-3 times larger than those previously known and their spatial distribution extends beyond the central OB associations. We propose a multi-object spectroscopy of these sources to confirm their nature. Using hydrogen and helium emission/absorption lines in the 4000-5000 A spectra and comparing with the OB spectral atlas in the literature, we can determine spectral subtypes and luminosity classes of these sources.

  1. obs4MIPS: Satellite Datasets for Model Evaluation

    NASA Astrophysics Data System (ADS)

    Ferraro, R.; Waliser, D. E.; Gleckler, P. J.

    2013-12-01

    This poster will review the current status of the obs4MIPs project, whose purpose is to provide a limited collection of well-established and documented datasets for comparison with Earth system models. These datasets have been reformatted to correspond with the CMIP5 model output requirements, and include technical documentation specifically targeted for their use in model output evaluation. There are currently over 50 datasets containing observations that directly correspond to CMIP5 model output variables. We will review the rational and requirements for obs4MIPs contributions, and provide summary information of the current obs4MIPs holdings on the Earth System Grid Federation. We will also provide some usage statistics, an update on governance for the obs4MIPs project, and plans for supporting CMIP6.

  2. BOREAS TF-9 SSA-OBS Branch Level Flux Data

    NASA Technical Reports Server (NTRS)

    Rayment, Mark B.; Jarvis, Paul G.; Hall, Forrest G. (Editor); Huemmrich, Karl (Editor)

    2000-01-01

    The BOREAS TF-9 team collected data that describe carbon dioxide and water vapor fluxes from foliage at the BOREAS SSA-OBS site from 07-April through 23-November-1996. The data are available in tabular ASCII files.

  3. Cygnus OB2: Star Formation Ugly Duckling Causes a Flap

    NASA Astrophysics Data System (ADS)

    Drake, Jeremy J.; Wright, Nicholas; Guarcello, Mario

    2015-08-01

    Cygnus OB2 is one of the largest known OB associations in our Galaxy, with a total stellar mass of 30,000 Msun and boasting an estimated 65 O-type stars and hundreds of OB stars. At a distance of only 1.4kpc, it is also the closest truly massive star forming region and provides a valuable testbed for star and planet formation theory. We have performed a deep stellar census using observations from X-ray to infrared, which has enabled studies of sub-structuring, mass segregation and dynamics, while infrared data reveal a story of protoplanetary disk attrition in an extremely harsh radiation environment. I will discuss how Cygnus OB2 challenges the idea that stars must form in dense, compact clusters, and demonstrates that stars as massive as 100 Msun can form in relatively low-density environments. Convincing evidence of disk photoevaporation poses a potential problem for planet formation and growth in starburst environments.

  4. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    This pair of true- and false-color images shows flooding along the Ob' (large east-west running river) and Irtysh (southern tributary of the Ob') on July 7, 2002. In the false-color image, land surfaces are orange-gold and flood waters are black or dark blue. Fires are marked with red dots in both images. Rivers Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  5. Preliminary performance report of the RHUM-RUM OBS network

    NASA Astrophysics Data System (ADS)

    Stähler, Simon C.; Crawford, Wayne; Barruol, Guilhem; Sigloch, Karin; Mechita, Schmidt-Aursch

    2015-04-01

    RHUM-RUM is a German-French seismological experiment based on the seafloor surrounding the hotspot of La Réunion, western Indian Ocean. Its primary objective is to clarify the presence or absence of a mantle plume beneath the Reunion hotspot. RHUM-RUM's central component is a one-year deployment (Oct 2012 - Nov 2013) of 57 broadband ocean-bottom seismometers (OBS) and hydrophones on an area of 2000x2000 km2 surrounding the hotspot. The OBS pool contained 48 instruments from the German DEPAS pool and 9 French stations from INSU. All OBS have been successfully recovered. Preliminary analysis of the seismometer recordings show large differences in long-period (>10s) noise levels between the German and the French OBS. These differences are strongest on the horizontal components and can be probably explained by dynamic tilt of the instrument itself. The noise level of the German instruments is >20dB higher in this period range compared to the French ones. A reason could be that for the German OBS, the seismometer is integrated into the OBS frame and therefore affected by its movement due to currents. The high noise level on the horizontal components will have to be considered in future experiment design, when using this instrument type for three-component waveform tomography.

  6. Quantifying the Components of the Field OB Star Population

    NASA Astrophysics Data System (ADS)

    Chen, Xinyi; Oey, M. S.; Lamb, Joel B.; Kushner, Cole

    2016-01-01

    Massive stars have long been thought to form in clusters. However, recent observations suggest some massive stars form in extremely sparse environments. Could these massive stars form alone? Clarifying the nature of field OB stars is central to this question. Defining these as OB stars that are isolated from other OB stars, the field comprises three distinct populations: "tip-of-the-iceberg stars", which are in small, but normal, clusters that only have one OB star; runaway stars; and any stars that form in situ in the field. The relative frequencies of each category are unknown. To study the population and obtain estimates of the frequencies, we examine 134 field OB stars from the Runaways and Isolated O-Type Star Spectroscopic Survey of the SMC (RIOTS4; Lamb et al. 2015) in the bar region of the Small Magellanic Cloud. These stars are at least 28 pc in projection away from other OB stars. We use OGLE-III (Udalski et al. 2008) I-band images to search for small, potential clusters around our target stars, using friends-of-friends and nearest-neighbor algorithms. We estimate the relative frequencies of tip-of-the-iceberg stars and isolated stars, some of which may have formed in situ. Thanks to Radek Poleski for help with the OGLE-III database. This work was supported by the NSF grants AST-0907758 and AST-1514838.

  7. Brown Adipose Tissue Transplantation Reverses Obesity in Ob/Ob Mice.

    PubMed

    Liu, Xiaomeng; Wang, Siping; You, Yilin; Meng, Minghui; Zheng, Zongji; Dong, Meng; Lin, Jun; Zhao, Qianwei; Zhang, Chuanhai; Yuan, Xiaoxue; Hu, Tao; Liu, Lieqin; Huang, Yuanyuan; Zhang, Lei; Wang, Dehua; Zhan, Jicheng; Jong Lee, Hyuek; Speakman, John R; Jin, Wanzhu

    2015-07-01

    Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted BAT from C57/BL6 mice into the dorsal subcutaneous region of age- and sex-matched leptin deficient Ob/Ob mice. Interestingly, BAT transplantation led to a significant reduction of body weight gain with increased oxygen consumption and decreased total body fat mass, resulting in improvement of insulin resistance and liver steatosis. In addition, BAT transplantation increased the level of circulating adiponectin, whereas it reduced the levels of circulating free T3 and T4, which regulate thyroid hormone sensitivity in peripheral tissues. BAT transplantation also increased β3-adrenergic receptor and fatty acid oxidation related gene expression in subcutaneous and epididymal (EP) white adipose tissue. Accordingly, BAT transplantation increased whole-body thermogenesis. Taken together our results demonstrate that BAT transplantation may reduce obesity and its related diseases by activating endogenous BAT. PMID:25830704

  8. Gender-Specific Metabolomic Profiling of Obesity in Leptin-Deficient ob/ob Mice by 1H NMR Spectroscopy

    PubMed Central

    Kim, Sang-Woo; Jung, Youngae; Bae, Hyun-Whee; Lee, Daeyoup; Park, Sung Goo; Lee, Chul-Ho; Hwang, Geum-Sook; Chi, Seung-Wook

    2013-01-01

    Despite the numerous metabolic studies on obesity, gender bias in obesity has rarely been investigated. Here, we report the metabolomic analysis of obesity by using leptin-deficient ob/ob mice based on the gender. Metabolomic analyses of urine and serum from ob/ob mice compared with those from C57BL/6J lean mice, based on the 1H NMR spectroscopy in combination with multivariate statistical analysis, revealed clear metabolic differences between obese and lean mice. We also identified 48 urine and 22 serum metabolites that were statistically significantly altered in obese mice compared to lean controls. These metabolites are involved in amino acid metabolism (leucine, alanine, ariginine, lysine, and methionine), tricarbocylic acid cycle and glucose metabolism (pyruvate, citrate, glycolate, acetoacetate, and acetone), lipid metabolism (cholesterol and carnitine), creatine metabolism (creatine and creatinine), and gut-microbiome-derived metabolism (choline, TMAO, hippurate, p-cresol, isobutyrate, 2-hydroxyisobutyrate, methylamine, and trigonelline). Notably, our metabolomic studies showed distinct gender variations. The obese male mice metabolism was specifically associated with insulin signaling, whereas the obese female mice metabolism was associated with lipid metabolism. Taken together, our study identifies the biomarker signature for obesity in ob/ob mice and provides biochemical insights into the metabolic alteration in obesity based on gender. PMID:24098417

  9. Unexpected effects of voluntary exercise training on natriuretic peptide and receptor mRNA expression in the ob/ob mouse heart.

    PubMed

    Broderick, Tom L; Wang, Donghao; Jankowski, Marek; Gutkowska, Jolanta

    2014-01-10

    Regular exercise is generally recommended for the treatment of obesity and type 2 diabetes. Exercise reduces body weight, improves glycemic control and cardiovascular (CV) function. This study was designed to determine the impact of voluntary wheel running on the cardiac oxytocin (OT)-natriuretic peptide (NP) system and plasma CV risk factors in the ob/ob mouse, a model of insulin resistance coupled with severe obesity. Five-week-old male ob/ob mice and non-obese heterozygote control littermates were assigned to either a sedentary or running group. Voluntary running was performed using a wheel system for a period of 8 weeks. Compared to non-obese mice, daily running activity expressed in kilometers, was significantly lower in ob/ob mice. In these mice, voluntary running improved body weight, but exacerbated CV markers, including plasma glucose and triglyceride levels. OT receptor gene expression was decreased in hearts of ob/ob mice compared to non-obese mice, and no improvement in the expression of this receptor was observed after voluntary running. Hearts from ob/ob mice also expressed lower BNP mRNA, whereas no differences in A- and C-type NP were observed between non-obese and ob/ob mice. After voluntary running, a downregulation in the expression of all three NPs coupled with increased apoptosis was observed in ob/ob hearts. Our results show that voluntary exercise running activity was decreased in the ob/ob mouse. Surprisingly, this was associated with a worsening of common CV plasma markers, reduced expression of peptides linked to the cardioprotective OT-NP system, and increased expression of cardiac apoptotic markers. PMID:24365091

  10. Effects of Cyclo-His-Pro-enriched yeast hydrolysate on blood glucose levels and lipid metabolism in obese diabetic ob/ob mice

    PubMed Central

    Jung, Eun Young; Hong, Yang Hee; Park, Chung

    2016-01-01

    BACKGROUND/OBJECTIVES We examined the hypoglycemic and anti-hyperlipidemic effect of yeast hydrolysate (YH) enriched with Cyclo-His-Pro (CHP) in the C57BL/6J ob/ob mouse model. MATERIALS/METHODS Mice were separated into 4 groups (8 mice/group) on the basis of blood glucose and body weight: WT control, lean mice given vehicle; ob/ob control, ob/ob mice given vehicle; YH-1, ob/ob mice given 0.5 g/kg of YH; YH-2, ob/ob mice given 1 g/kg of YH. YH in saline or vehicle was administered orally in the same volume every day for 3 weeks. RESULTS Mice treated with YH (0.5 and 1 g/kg) for 3 weeks displayed a significant reduction in overall body weight gain and perirenal and epididymal adipose tissue weight compared to the ob/ob control group. Additionally, high-density lipoprotein (HDL) cholesterol, glucose, and atherogenic indexes were significantly decreased in the blood of YH-1 and YH-2 groups compared to the ob/ob control. In ob/ob mice, YH administration significantly improved glucose tolerance and blood insulin levels. These data indicate that YH treatment produces potent hypoglycemic and anti-hyperlipidemic effects by controlling body weight, fat mass, blood lipid, insulin levels, and glucose tolerance. CONCLUSION YH could potentially be used as a treatment option for diabetes and hyperlipidemia. The CHP-enriched YH may be a promising strategy in the development of hypoglycemic peptide nutraceuticals. PMID:27087898

  11. Hypothalamic Leptin Gene Therapy Reduces Bone Marrow Adiposity in ob/ob Mice Fed Regular and High-Fat Diets

    PubMed Central

    Lindenmaier, Laurence B.; Philbrick, Kenneth A.; Branscum, Adam J.; Kalra, Satya P.; Turner, Russell T.; Iwaniec, Urszula T.

    2016-01-01

    Low bone mass is often associated with elevated bone marrow adiposity. Since osteoblasts and adipocytes are derived from the same mesenchymal stem cell (MSC) progenitor, adipocyte formation may increase at the expense of osteoblast formation. Leptin is an adipocyte-derived hormone known to regulate energy and bone metabolism. Leptin deficiency and high-fat diet-induced obesity are associated with increased marrow adipose tissue (MAT) and reduced bone formation. Short-duration studies suggest that leptin treatment reduces MAT and increases bone formation in leptin-deficient ob/ob mice fed a regular diet. Here, we determined the long-duration impact of increased hypothalamic leptin on marrow adipocytes and osteoblasts in ob/ob mice following recombinant adeno-associated virus (rAAV) gene therapy. Eight- to 10-week-old male ob/ob mice were randomized into four groups: (1) untreated, (2) rAAV-Lep, (3) rAAV-green fluorescent protein (rAAV-GFP), or (4) pair-fed to rAAV-Lep. For vector administration, mice were injected intracerebroventricularly with either rAAV-leptin gene therapy (rAAV-Lep) or rAAV-GFP (9 × 107 particles) and maintained for 30 weeks. In a second study, the impact of increased hypothalamic leptin levels on MAT was determined in mice fed high-fat diets; ob/ob mice were randomized into two groups and treated with either rAAV-Lep or rAAV-GFP. At 7 weeks post-vector administration, half the mice in each group were switched to a high-fat diet for 8 weeks. Wild-type (WT) controls included age-matched mice fed regular or high-fat diet. High-fat diet resulted in a threefold increase in MAT in WT mice, whereas MAT was increased by leptin deficiency up to 50-fold. Hypothalamic leptin gene therapy increased osteoblast perimeter and osteoclast perimeter with minor change in cancellous bone architecture. The gene therapy decreased MAT levels in ob/ob mice fed regular or high-fat diet to values similar to WT mice fed regular diet. These findings suggest

  12. Hypothalamic Leptin Gene Therapy Reduces Bone Marrow Adiposity in ob/ob Mice Fed Regular and High-Fat Diets.

    PubMed

    Lindenmaier, Laurence B; Philbrick, Kenneth A; Branscum, Adam J; Kalra, Satya P; Turner, Russell T; Iwaniec, Urszula T

    2016-01-01

    Low bone mass is often associated with elevated bone marrow adiposity. Since osteoblasts and adipocytes are derived from the same mesenchymal stem cell (MSC) progenitor, adipocyte formation may increase at the expense of osteoblast formation. Leptin is an adipocyte-derived hormone known to regulate energy and bone metabolism. Leptin deficiency and high-fat diet-induced obesity are associated with increased marrow adipose tissue (MAT) and reduced bone formation. Short-duration studies suggest that leptin treatment reduces MAT and increases bone formation in leptin-deficient ob/ob mice fed a regular diet. Here, we determined the long-duration impact of increased hypothalamic leptin on marrow adipocytes and osteoblasts in ob/ob mice following recombinant adeno-associated virus (rAAV) gene therapy. Eight- to 10-week-old male ob/ob mice were randomized into four groups: (1) untreated, (2) rAAV-Lep, (3) rAAV-green fluorescent protein (rAAV-GFP), or (4) pair-fed to rAAV-Lep. For vector administration, mice were injected intracerebroventricularly with either rAAV-leptin gene therapy (rAAV-Lep) or rAAV-GFP (9 × 10(7) particles) and maintained for 30 weeks. In a second study, the impact of increased hypothalamic leptin levels on MAT was determined in mice fed high-fat diets; ob/ob mice were randomized into two groups and treated with either rAAV-Lep or rAAV-GFP. At 7 weeks post-vector administration, half the mice in each group were switched to a high-fat diet for 8 weeks. Wild-type (WT) controls included age-matched mice fed regular or high-fat diet. High-fat diet resulted in a threefold increase in MAT in WT mice, whereas MAT was increased by leptin deficiency up to 50-fold. Hypothalamic leptin gene therapy increased osteoblast perimeter and osteoclast perimeter with minor change in cancellous bone architecture. The gene therapy decreased MAT levels in ob/ob mice fed regular or high-fat diet to values similar to WT mice fed regular diet. These findings suggest

  13. OB protein binds specifically to the choroid plexus of mice and rats.

    PubMed Central

    Devos, R; Richards, J G; Campfield, L A; Tartaglia, L A; Guisez, Y; van der Heyden, J; Travernier, J; Plaetinck, G; Burn, P

    1996-01-01

    Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissue from lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats were probed to identify potential central OB protein-binding sites. We report here that recombinant OB protein binds specifically to the choroid plexus. The binding of OB protein (either radiolabeled or the alkaline phosphatase-OB fusion protein) and its displacement by unlabeled OB protein was similar in lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats. These findings suggest that OB protein binds with high affinity to a specific receptor in the choroid plexus. After binding to the choroid plexus receptor, OB protein may then be transported across the blood-brain barrier into the cerebrospinal fluid. Alternatively, binding of OB protein to a specific receptor in the choroid plexus may activate afferent neural inputs to the neural network that regulates feeding behavior and energy balance or may result in the clearance or degradation of OB protein. The identification of the choroid plexus as a brain binding site for OB protein will provide the basis for the construction of expression libraries and facilitate the rapid cloning of the choroid plexus OB receptor. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8643634

  14. OB protein binds specifically to the choroid plexus of mice and rats.

    PubMed

    Devos, R; Richards, J G; Campfield, L A; Tartaglia, L A; Guisez, Y; van der Heyden, J; Travernier, J; Plaetinck, G; Burn, P

    1996-05-28

    Binding studies were conducted to identify the anatomical location of brain target sites for OB protein, the ob gene product. 125I-labeled recombinant mouse OB protein or alkaline phosphatase-OB fusion proteins were used for in vitro and in vivo binding studies. Coronal brain sections or fresh tissue from lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats were probed to identify potential central OB protein-binding sites. We report here that recombinant OB protein binds specifically to the choroid plexus. The binding of OB protein (either radiolabeled or the alkaline phosphatase-OB fusion protein) and its displacement by unlabeled OB protein was similar in lean, obese ob/ob, and obese db/db mice as well as lean and obese Zucker rats. These findings suggest that OB protein binds with high affinity to a specific receptor in the choroid plexus. After binding to the choroid plexus receptor, OB protein may then be transported across the blood-brain barrier into the cerebrospinal fluid. Alternatively, binding of OB protein to a specific receptor in the choroid plexus may activate afferent neural inputs to the neural network that regulates feeding behavior and energy balance or may result in the clearance or degradation of OB protein. The identification of the choroid plexus as a brain binding site for OB protein will provide the basis for the construction of expression libraries and facilitate the rapid cloning of the choroid plexus OB receptor. PMID:8643634

  15. Red supergiants and the past of Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Comerón, F.; Djupvik, A. A.; Schneider, N.; Pasquali, A.

    2016-02-01

    Context. Red supergiants are the evolved descendants of massive stars with initial masses between 7 and 40 M⊙. Their brightness makes them easily detectable in the near infrared, making them useful probes of star formation that occurred several tens of Myr ago. Aims: We investigate the past star formation history of Cygnus OB2, the nearest very massive OB association, using red supergiants as a probe. Our aim is to confirm the evidence, found by previous studies, that star formation in the Cygnus OB2 region started long before the latest burst that gave rise to the dense aggregate of early O-type stars that dominate the appearance of the association at present. Methods: Near-infrared star counts in the Cygnus region reveal moderate evidence for a peak in the areal density of bright, reddened stars approximately coincident with Cygnus OB2. A total of 11 sources are found within a circle of 1° radius centered on the association, of which 4 are non-supergiants based on existing observations. Near-infrared spectroscopy is presented of the remaining seven candidates, including four that have been already classified as M supergiants in the literature. Results: We confirm the presence of seven red supergiants in the region and argue that they are probably physically associated with Cygnus OB2. Their location is roughly coincident with that of the older population identified by previous studies, supporting the scenario in which the main star formation activity in the association has been shifting toward higher Galactic longitudes with time. Their luminosities are compared with the predictions of evolutionary tracks with and without rotation to estimate the mass of their progenitors and ages. In this way, we confirm that massive star formation was already taking place in the area of Cygnus OB2 over 20 Myr ago, and we estimate that the star formation rate in the latest 6 Myr represents a six-fold increase over the massive star formation rate at the time when the

  16. No evidence of disk destruction by OB stars

    NASA Astrophysics Data System (ADS)

    Richert, Alexander J. W.; Feigelson, Eric

    2015-01-01

    It has been suggested that the hostile environments observed in massive star forming regions are inhospitable to protoplanetary disks and therefore to the formation of planets. The Orion Proplyds show disk evaporation by extreme ultraviolet (EUV) photons from Theta1 Orionis C (spectral type O6). In this work, we examine the spatial distributions of disk-bearing and non-disk bearing young stellar objects (YSOs) relative to OB stars in 17 massive star forming regions in the MYStIX (Massive Young Star-Forming Complex Study in Infrared and X-ray) survey. Any tendency of disky YSOs, identified by their infrared excess, to avoid OB stars would reveal complete disk destruction.We consider a sample of MYStIX that includes 78 O3-O9 stars, 256 B stars, 5,606 disky YSOs, and 5,794 non-disky YSOs. For each OB star, we compare the cumulative distribution functions of distances to disky and non-disky YSOs. We find no significant avoidance of OB stars by disky YSOs. This result indicates that OB stars are not sufficiently EUV-luminous and long-lived to completely destroy a disk within its ordinary lifetime. We therefore conclude that massive star forming regions are not clearly hostile to the formation of planets.

  17. Obs4MIPS: Satellite Observations for Model Evaluation

    NASA Astrophysics Data System (ADS)

    Ferraro, R.; Waliser, D. E.; Gleckler, P. J.

    2015-12-01

    This poster will review the current status of the obs4MIPs project, whose purpose is to provide a limited collection of well-established and documented datasets for comparison with Earth system models (https://www.earthsystemcog.org/projects/obs4mips/). These datasets have been reformatted to correspond with the CMIP5 model output requirements, and include technical documentation specifically targeted for their use in model output evaluation. There are currently over 50 datasets containing observations that directly correspond to CMIP5 model output variables. We will review recent additions to the obs4MIPs collection, and provide updated download statistics. We will also provide an update on changes to submission and documentation guidelines, the work of the WCRP Data Advisory Council (WDAC) Observations for Model Evaluation Task Team, and engagement with the CMIP6 MIP experiments.

  18. e-MERLIN 21cm constraints on the mass-loss rates of OB stars in Cyg OB2

    NASA Astrophysics Data System (ADS)

    Morford, J. C.; Fenech, D. M.; Prinja, R. K.; Blomme, R.; Yates, J. A.

    2016-08-01

    We present e-MERLIN 21 cm (L-band) observations of single luminous OB stars in the Cygnus OB2 association, from the COBRaS Legacy programme. The radio observations potentially offer the most straightforward, least model-dependent, determinations of mass-loss rates, and can be used to help resolve current discrepancies in mass-loss rates via clumped and structured hot star winds. We report here that the 21 cm flux densities of O3 to O6 supergiant and giant stars are less than ˜ 70 μJy. These fluxes may be translated to `smooth' wind mass-loss upper limits of ˜ 4.4 - 4.8 × 10-6 M⊙ yr -1 for O3 supergiants and ≲ 2.9 × 10-6 M⊙ yr -1 for B0 to B1 supergiants. The first ever resolved 21 cm detections of the hypergiant (and LBV candidate) Cyg OB2 #12 are discussed; for multiple observations separated by 14 days, we detect a ˜ 69% increase in its flux density. Our constraints on the upper limits for the mass-loss rates of evolved OB stars in Cyg OB2 support the model that the inner wind region close to the stellar surface (where Hα forms) is more clumped than the very extended geometric region sampled by our radio observations.

  19. Modeling the winds and magnetospheres of active OB stars

    NASA Astrophysics Data System (ADS)

    Townsend, Richard H. D.

    2011-07-01

    After briefly reviewing the theory behind the radiative line-driven winds of OB stars, I examine the processes that can generate structure in them; these include both intrinsic instabilities, and surface perturbations such as pulsation and rotation. I then delve into wind channeling and confinement by magnetic fields as a mechanism for forming longer-lived circumstellar structures. With a narrative that largely follows the historical progression of the field, I introduce the key insights and results that link the first detection of a magnetosphere, over three decades ago, to the recent direct measurement of magnetic braking in a number of active OB stars.

  20. Depth Imaging of OBS Reflection Data With Wave Field Separation

    NASA Astrophysics Data System (ADS)

    Asakawa, E.; Mizohata, S.; Tanaka, H.; Mikada, H.; Nishizawa, A.

    2007-12-01

    We propose a newly-developed depth imaging approach for OBS (Ocean Bottom Seismometer) reflection data in active-source structural survey using wavefield separation and PSDM (Prestack Depth Migration). OBS data includes a lot of valuable signals not only reflection but refraction. However its wavefield is contaminated with a various kind of waves that degrade the quality of the depth imaging of OBS reflection. Water reverberations, for example, have been thought as a strong source of noise in the imaging. Surprisingly, we found that the multiples reflection waves have the wide spreads of reflection points and we take advantage of this feature to improve the depth imaging after careful processing acquired OBS data. We would like to demonstrate that multiples could be utilized to enhance signal-to-noise ratio. The processing of OBS data in this study is summarized as follows. First, we categorize the OBS wavefield into two parts, i.e., near and far offset data. The near and far offset data inhere are waves that arrive after and before the direct water arrival, respectively. Then we separate the both near and far wavefields into two parts at the arrival of the first-order multiple. The reflection signals before the multiple are primary and up-going waves, whereas the reflection after the multiple events are mainly multiple and down-going in a common receiver gather. After these time-based separations, we apply up/downgoing wave field separation using geophone vertical-component and hydrophone data. Hydrophone records water pressure and, hence, are omni-directional while the vertical component of geophone measures a component of the vector response. These characteristic difference leads us to the separation of upgoing primary reflections and downgoing multiples using the polarity differences due to propagation direction of incoming waves. Finally, we obtain the OBS reflections to 4 domains, near offset primary, near offset multiple, far offset primary and far offset

  1. Marine TAIGER OBS Experiment and its future prospects

    NASA Astrophysics Data System (ADS)

    Lee, C.; Wang, T.; van Avendonk, H. J.; Huang, Y.; Lin, J.; Lallemand, S.; Klingelhoeher, F.

    2009-12-01

    A total of 260 OBSs were deployed in the marine TAIGER program from late March to late July, 2009. These data were collected by US Columbia University’s R/V Langseth as the big-power seismic shooting ship and 10 Taiwanese ships to take terms for supporting of the OBS experiment in the entire seismic cruises. The OBS were provided by the National Taiwan Ocean University, French IFREMER and Scripps Institution of Oceanography. During these 4 months, we have worked around Taiwan in the South China Sea, Luzon Arc, East Taiwan and West Philippine Basin. All efforts are put together by many earth scientists from Taiwan, USA and France under one major purpose, to get a better understanding of the Taiwan mountain building processes. As a result, these new data will provide as a base to combine with many other disciplinal studies, such as the multi-channel seismic, land recorded seismometer data, gravity and magnetic as well as the natural earthquake data recorded by the OBS during the experiment time. Four very preliminary OBS data analyses will be presented in the same T25 postal section. Beside the research, we also carried out our teaching to our students on board a Taiwanese student training ship, Yu-Yin No.2. Therefore, an educational post is also to be shown in the ED01 section. Even the data analyses are in an early stage, but we are exciting about it. For example, 3 OBS profiles (T4, T5 and T6) in the East Taiwan were shot twice in normal and reversed directions with different shot intervals (30 and 60 seconds per shot). This exercise will be important to interpret the complicate collision/subduction structures in the East Taiwan. Two OBS profiles (T1 and T2) in the Luzon Arc were shot 5 times in the separated R/V Langseth cruises (due to the typhoon effects), again with different shot intervals (20 and 60 seconds per shot). These will provide us more opportunities to examine the collisional features in between Taiwan and Luzon. One OBS long profile (550 km) was

  2. All-trans retinoic acid stimulates gene expression of the cardioprotective natriuretic peptide system and prevents fibrosis and apoptosis in cardiomyocytes of obese ob/ob mice.

    PubMed

    Manolescu, Daniel-Constantin; Jankowski, Marek; Danalache, Bogdan A; Wang, Donghao; Broderick, Tom L; Chiasson, Jean-Louis; Gutkowska, Jolanta

    2014-10-01

    In hypertensive rodents, retinoic acid (RA) prevents adverse cardiac remodelling and improves myocardial infarction outcome, but its role in obesity-related changes of cardiac tissue are unclear. We hypothesized that all-trans RA (ATRA) treatment will improve the cardioprotective oxytocin-natriuretic peptides (OT-NP) system, preventing apoptosis and collagen accumulation in hearts of ob/ob mice, a mouse model of obesity and insulin resistance. Female 9-week-old B6.V-Lep/J ob/ob mice (n = 16) were divided into 2 groups: 1 group (n = 8) treated with 100 μg of ATRA dissolved in 100 μL of corn oil (vehicle) delivered daily (∼2 μg·g body weight(-1)·day(-1)) by stomach intubation for 16 days, and 1 group (n = 8) that received the vehicle alone. A group of nonobese littermate mice (n = 9) served as controls. Ob/ob mice exhibited obesity, hyperglycaemia, and downregulation of the cardiac OT-NP system, including the mRNA for the transcription factor GATA4, OT receptor and brain NP, and the protein expression for endothelial nitric oxide synthase. Hearts from ob/ob mice also demonstrated increased apoptosis and collagen accumulation. ATRA treatment induced weight loss and decreased adipocytes diameter in the visceral fat, thus reducing visceral obesity, which is associated with a high risk for cardiovascular disease. RA treatment was associated with a reduction in hyperglycemia and a normalization of the OT-NP system's expression in the hearts of ob/ob mice. Furthermore, ATRA treatment prevented apoptosis and collagen accumulation in hearts of ob/ob mice. The present study indicates that ATRA treatment was effective in restoring the cardioprotective OT-NP system and in preventing abnormal cardiac remodelling in the ob/ob mice. PMID:25017112

  3. Acute intravenous leptin infusion increases glucose turnover but not skeletal muscle glucose uptake in ob/ob mice.

    PubMed

    Burcelin, R; Kamohara, S; Li, J; Tannenbaum, G S; Charron, M J; Friedman, J M

    1999-06-01

    The mouse ob gene encodes leptin, an adipocyte hormone that regulates body weight and energy expenditure. Leptin has potent metabolic effects on fat and glucose metabolism. A mutation of the ob gene results in mice with severe hereditary obesity and diabetes that can be corrected by treatment with the hormone. In lean mice, leptin acutely increases glucose metabolism in an insulin-independent manner, which could account, at least in part, for some of the antidiabetic effect of the hormone. To investigate further the acute effect of leptin on glucose metabolism in insulin-resistant obese diabetic mice, leptin (40 ng x g(-1) x h(-1)) was administered intravenously for 6 h in C57Bl/6J ob/ob mice. Leptin increased glucose turnover and stimulated glucose uptake in brown adipose tissue (BAT), brain, and heart with no increase in heart rate. A slight increase in all splanchnic tissues was also noticed. Conversely, no increase in skeletal muscle or white adipose tissue (WAT) glucose uptake was observed. Plasma insulin concentration increased moderately but neither glucose, glucagon, thyroid hormones, growth hormone, nor IGF-1 levels were different from phosphate-buffered saline-infused C57Bl/6J ob/ob mice. In addition, leptin stimulated hepatic glucose production, which was associated with increased glucose-6-phosphatase activity. Conversely, PEPCK activity was rather diminished. Interestingly, hepatic insulin receptor substrate (IRS)1-associated phosphatidylinositol 3-kinase activity was slightly elevated, but neither the content of glucose transporter GLUT2 nor the phosphorylation state of the insulin receptor and IRS-1 were changed by acute leptin treatment. Hepatic lipid metabolism was not stimulated during the acute leptin infusion, since the content of triglycerides, glycerol, and citrate was unchanged. These findings suggest that in ob/ob mice, the antidiabetic antiobesity effect of leptin could be the result of a profound alteration of glucose metabolism in liver

  4. The Massive Star Forming Region Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas James; Drake, J. J.; Drew, J. E.

    2009-12-01

    We present a multi-wavelength study of the massive star forming Cygnus OB2. Cygnus OB2 is the northern hemisphere's most massive star forming region and hosts a tremendously rich and diverse stellar population, with thousands of OB stars. The strong and highly variable extinction in the direction of the association have hindered previous studies of the region, but recent deep photometric surveys in the optical and near-infrared are opening the region up for study. The appreciation of the pivotal status of Cyg OB2 has led to a number of recent ambitious surveys of the cluster and its setting within the Cygnus-X region at X-ray, infrared and radio wavelengths. Chandra X-ray observations of two fields in the center of the association reveal 1720 X-ray sources, which we have combined with optical and near-IR photometry from the IPHAS and UKIDSS surveys. Near-IR photometry reveals a stellar population with a spread of ages greater than previously thought, overturning the picture of coeval star formation in the region. The distribution of young sources in the region shows evidence for clustering and significant mass segregation, which we judge to be primordial given the cluster's age.

  5. Obs4MIPS: Satellite Observations for CMIP6 Model Evaluation

    NASA Astrophysics Data System (ADS)

    Ferraro, R.; Waliser, D. E.; Gleckler, P. J.; Taylor, K. E.; Eyring, V.

    2014-12-01

    This poster will review the current status of the obs4MIPs project, whose purpose is to provide a limited collection of well-established and documented datasets for comparison with Earth system models (https://www.earthsystemcog.org/projects/obs4mips/). These datasets have been reformatted to correspond with the CMIP5 model output requirements, and include technical documentation specifically targeted for their use in model otput evaluation. There are currently over 50 datasets containing observations that directly correspond to CMIP5 model output variables. We will review the results and recommendations from the recent obs4MIPs - CMIP6 planning meeting, which gathered over 50 experts in satellite observations and CMIP modeling, to assess the needed observations to support the next round of CMIP experiments. The recommendations address key issues regarding the inclusion of higher frequency datasets (both observations and model output), the need for error and bias characterization, the inclusion of reanalysis, and support for observation simulators. An update on the governance for the obs4MIPs project and recent usage statistics will also be presented.

  6. Recent Seismic Experiments of OBS in the South China Sea

    NASA Astrophysics Data System (ADS)

    Ruan, A.; Li, J.; Wu, Z.

    2012-12-01

    Since 2006 some research institutions of China have carried out some important seismic experiments by using ocean bottom seismometer(OBS) in the South China Sea (SCS) and obtained many concrete progresses in modeling the crustal structure of SCS and also in understanding of its formation and evolution as well. In 2006 three wide-angle profiles were completed in the northern margin, named OBS2006-1 across the northwestern sub-basin, OBS2006-2 parallel to the sea basin boundary and OBS2006-3 across the Dongsha Rise and Chaoshan Depression respectively. In 2010 two wide- angle profiles were completed, named OBS2010-1 and OBS 2010-2 both perpendicular to the northern off-shore faulting system. During 2009-2011 four wide-angle profiles were completed in the southern margin, named OBS973-1 from southern margin to the southwestern sub-basin, OBS973-2 from Liyue Bank to the southwestern sub-basin and OBS973-3 from Xisha to the southwestern sub-basin, OBS2011-2 from Xisha to Hainan Island respectively. In 2011 two 3D seismic array of OBS were completed in the Zhongnan-Changlong sea mount chain and Huangyan-Zhenbei sea mount chain respectively. Here we present some primary but important results as follows. (1) The velocity model of OBS2006-1 indicates that the crust under the continental slope decreases from 21km to 11km, and to 7.7km in the northwestern sub-basin with Moho depth ascends from 21km to 11km. The tectonic geometry and velocity structure of the northwestern sub-basin and its margins on both sides shows symmetrical and conjugate and indicates pure shear mode of continental margin rifting mechanism. (2) The velocity model of OBS2006-3 reveals remarkable thickness with maximum 8 km of the Mesozoic sediment in Chaoshan Depression in which velocity increases downward from 4.4 km/s at top to 5.3 km/s at the bottom. The buried depth of Moho decreases from 24-25 km under Dongsha Rise to 17 km in the lower slope and an obviously velocity abnormal is detected in the upper

  7. A Search for Pulsar Companions to OB Runaway Stars

    NASA Astrophysics Data System (ADS)

    Sayer, R. W.; Nice, D. J.; Kaspi, V. M.

    1996-04-01

    We have searched for radio pulsar companions to 40 nearby OB runaway stars. Observations were made at 575 and 770 MHz with the NRAO 140 foot (43 m) telescope. The survey was sensitive to long- period pulsars with flux densities of 1 mJy or more. No pulsar companions to OB runaways were discovered. One previously unknown pulsar was discovered, PSR J2044 +4614, while observing toward target 0 star BD +45°3260. Follow-up timing observations of the pulsar measured its position to high precision, revealing a 9' separation between the pulsar and the target star, unequivocally indicating they are not associated. The pulsar is ordinary, except that its dispersion measure, 315 pc cm-3, is unusually high given its Galactic longitude, l = 85°. The nondetection of pulsars bound to target stars places an upper limit of 8% (95% confidence) for the fraction of runaway OB stars with pulsar companions detectable via pulsed radio emission by surveys such as ours. Assuming standard models for the pulsar beaming fraction and luminosity function, we conclude most OB runaways do not have pulsar companions.

  8. OB/OOCH Upstream 2007: Reflections on an Upstream Journey

    ERIC Educational Resources Information Center

    Edmonstone, Laura

    2008-01-01

    In this article, the author shares her experience of spending time at Camp Oochigeas as an Outward Bound (OB) instructor. The author was there as part of a team, working in a program that partnered three strong organizations with a wealth of knowledge and experience in working with kids. Camp Oochigeas operates a volunteer-based program for kids…

  9. New very massive stars in Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Negueruela, I.; Marco, A.; Herrero, A.; Clark, J. S.

    2008-08-01

    Context: The compact association Cygnus OB2 is known to contain a large population of massive stars, but its total mass is currently a matter of debate. While recent surveys have uncovered large numbers of OB stars in the area around Cyg OB2, detailed study of the optically brightest among them suggests that most are not part of the association. Aims: We observed an additional sample of optically faint OB star candidates, with the aim of checking if more obscured candidates are correspondingly more likely to be members of Cyg OB2. Methods: Low resolution spectra of 9 objects allow the rejection of one foreground star and the selection of four O-type stars, which were later observed at higher resolution. In a subsequent run, we observed three more stars in the classification region and three other stars in the far red. Results: We identify five (perhaps six) new evolved very massive stars and three main sequence O-type stars, all of which are likely to be members of Cyg OB2. The new findings allow a much better definition of the upper HR diagram, suggesting an age ~2.5 Myr for the association and hinting that the O3-5 supergiants in the association are blue stragglers, either younger or following a different evolutionary path from other cluster members. Though the bulk of the early stars seems to belong to an (approximately) single-age population, there is ample evidence for the presence of somewhat older stars at the same distance. Conclusions: Our results suggest that, even though Cyg OB2 is unlikely to contain as many as 100 O-type stars, it is indeed substantially more massive than was thought prior to recent infrared surveys. Figure [see full textsee full textsee full textsee full textsee full textsee full textsee full text] and Table [see full textsee full textsee full textsee full textsee full textsee full textsee full text] are only available in electronic form at http://www.aanda.org

  10. Deficient copper concentrations in dried-defatted hepatic tissue from ob/ob mice: A potential model for study of defective copper regulation in metabolic liver disease

    PubMed Central

    Church, Stephanie J.; Begley, Paul; Kureishy, Nina; McHarg, Selina; Bishop, Paul N.; Bechtold, David A.; Unwin, Richard D.; Cooper, Garth J.S.

    2015-01-01

    Ob/ob mice provide an animal model for non-alcoholic fatty liver disease/non-alcoholic steatohepatitis (NAFLD/NASH) in patients with obesity and type-2 diabetes. Low liver copper has been linked to hepatic lipid build-up (steatosis) in animals with systemic copper deficiency caused by low-copper diets. However, hepatic copper status in patients with NAFLD or NASH is uncertain, and a validated animal model useful for the study of hepatic copper regulation in common forms of metabolic liver disease is lacking. Here, we report parallel measurements of essential metal levels in whole-liver tissue and defatted-dried liver tissue from ob/ob and non-obese control mice. Measurements in whole-liver tissue from ob/ob mice at an age when they have developed NAFLD/NASH, provide compelling evidence for factitious lowering of copper and all other essential metals by steatosis, and so cannot be used to study hepatic metal regulation in this model. By marked contrast, metal measurements in defatted-dried liver samples reveal that most essential metals were actually normal and indicate specific lowering of copper in ob/ob mice, consistent with hepatic copper deficiency. Thus ob/ob mice can provide a model useful for the study of copper regulation in NAFLD and NASH, provided levels are measured in defatted-dried liver tissue. PMID:25797622

  11. ObsPy: A Python Toolbox for Seismology

    NASA Astrophysics Data System (ADS)

    Wassermann, J. M.; Krischer, L.; Megies, T.; Barsch, R.; Beyreuther, M.

    2013-12-01

    Python combines the power of a full-blown programming language with the flexibility and accessibility of an interactive scripting language. Its extensive standard library and large variety of freely available high quality scientific modules cover most needs in developing scientific processing workflows. ObsPy is a community-driven, open-source project extending Python's capabilities to fit the specific needs that arise when working with seismological data. It a) comes with a continuously growing signal processing toolbox that covers most tasks common in seismological analysis, b) provides read and write support for many common waveform, station and event metadata formats and c) enables access to various data centers, webservices and databases to retrieve waveform data and station/event metadata. In combination with mature and free Python packages like NumPy, SciPy, Matplotlib, IPython, Pandas, lxml, and PyQt, ObsPy makes it possible to develop complete workflows in Python, ranging from reading locally stored data or requesting data from one or more different data centers via signal analysis and data processing to visualization in GUI and web applications, output of modified/derived data and the creation of publication-quality figures. All functionality is extensively documented and the ObsPy Tutorial and Gallery give a good impression of the wide range of possible use cases. ObsPy is tested and running on Linux, OS X and Windows and comes with installation routines for these systems. ObsPy is developed in a test-driven approach and is available under the LGPLv3 open source licence. Users are welcome to request help, report bugs, propose enhancements or contribute code via either the user mailing list or the project page on GitHub.

  12. The massive star population of Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas J.; Drew, Janet E.; Mohr-Smith, Michael

    2015-05-01

    We have compiled a significantly updated and comprehensive census of massive stars in the nearby Cygnus OB2 association by gathering and homogenizing data from across the literature. The census contains 169 primary OB stars, including 52 O-type stars and 3 Wolf-Rayet stars. Spectral types and photometry are used to place the stars in a Hertzsprung-Russell diagram, which is compared to both non-rotating and rotating stellar evolution models, from which stellar masses and ages are calculated. The star formation history and mass function of the association are assessed, and both are found to be heavily influenced by the evolution of the most massive stars to their end states. We find that the mass function of the most massive stars is consistent with a `universal' power-law slope of Γ = 1.3. The age distribution inferred from stellar evolutionary models with rotation and the mass function suggest the majority of star formation occurred more or less continuously between 1 and 7 Myr ago, in agreement with studies of low- and intermediate-mass stars in the association. We identify a nearby young pulsar and runaway O-type star that may have originated in Cyg OB2 and suggest that the association has already seen its first supernova. Finally we use the census and mass function to calculate the total mass of the association of 16 500^{+3800}_{-2800} M⊙, at the low end, but consistent with, previous estimates of the total mass of Cyg OB2. Despite this Cyg OB2 is still one of the most massive groups of young stars known in our Galaxy making it a prime target for studies of star formation on the largest scales.

  13. Effects of caloric restriction on O-GlcNAcylation, Ca(2+) signaling, and learning impairment in the hippocampus of ob/ob mice.

    PubMed

    Jeon, Byeong Tak; Heo, Rok Won; Jeong, Eun Ae; Yi, Chin-Ok; Lee, Jong Youl; Kim, Kyung Eun; Kim, Hwajin; Roh, Gu Seob

    2016-08-01

    Diabetes may adversely affect cognitive function and, conversely, caloric restriction (CR) increases longevity and improves memory. To shed light on the unknown underlying mechanisms involved in these observations, we examined the effects of CR on serum metabolic parameters and hippocampal protein expression in the ob/ob mice model of obesity-induced diabetes. We found that CR reduced hepatic steatosis and insulin resistance in ob/ob mice. In addition, CR increased the levels of hippocampal O-linked-N-acetylglucosamine (O-GlcNAc) and GlcNAc transferase and decreased the expression of calcium/calmodulin-dependent protein kinase II, lipocalin-2, and phosphorylated tau. Furthermore, CR lessened the learning deficits that are typically seen in ob/ob mice. These findings indicate that CR may reverse obesity-related brain glucose impairment and intracellular Ca(2+) dysfunction and relieve learning impairment associated with diabetes. PMID:27318140

  14. Loss of FXR protects against diet-induced obesity and accelerates liver carcinogenesis in ob/ob mice.

    PubMed

    Zhang, Yanqiao; Ge, Xuemei; Heemstra, Lydia A; Chen, Wei-Dong; Xu, Jiesi; Smith, Joseph L; Ma, Huiyan; Kasim, Neda; Edwards, Peter A; Novak, Colleen M

    2012-02-01

    Farnesoid X receptor (FXR) is known to play important regulatory roles in bile acid, lipid, and carbohydrate metabolism. Aged (>12 months old) Fxr(-/-) mice also develop spontaneous liver carcinomas. In this report, we used three mouse models to investigate the role of FXR deficiency in obesity. As compared with low-density lipoprotein receptor (Ldlr) knockout (Ldlr(-/-)) mice, the Ldlr(-/-)Fxr(-/-) double-knockout mice were highly resistant to diet-induced obesity, which was associated with increased expression of genes involved in energy metabolism in the skeletal muscle and brown adipose tissue. Such a striking effect of FXR deficiency on obesity on an Ldlr(-/-) background led us to investigate whether FXR deficiency alone is sufficient to affect obesity. As compared with wild-type mice, Fxr(-/-) mice showed resistance to diet-induced weight gain. Interestingly, only female Fxr(-/-) mice showed significant resistance to diet-induced obesity, which was accompanied by increased energy expenditure in these mice. Finally, we determined the effect of FXR deficiency on obesity in a genetically obese and diabetic mouse model. We generated ob(-/-)Fxr(-/-) mice that were deficient in both Leptin and Fxr. On a chow diet, ob(-/-)Fxr(-/-) mice gained less body weight and had reduced body fat mass as compared with ob/ob mice. In addition, we observed liver carcinomas in 43% of young (<11 months old) Ob(-/-)Fxr(-/-) mice. Together these data indicate that loss of FXR prevents diet-induced or genetic obesity and accelerates liver carcinogenesis under diabetic conditions. PMID:22261820

  15. iPLA2β deficiency attenuates obesity and hepatic steatosis in ob/ob mice through hepatic fatty-acyl phospholipid remodeling.

    PubMed

    Deng, Xiuling; Wang, Jiliang; Jiao, Li; Utaipan, Tanyarath; Tuma-Kellner, Sabine; Schmitz, Gerd; Liebisch, Gerhard; Stremmel, Wolfgang; Chamulitrat, Walee

    2016-05-01

    PLA2G6 or GVIA calcium-independent PLA2 (iPLA2β) is identified as one of the NAFLD modifier genes in humans, and thought to be a target for NAFLD therapy. iPLA2β is known to play a house-keeping role in phospholipid metabolism and remodeling. However, its role in NAFLD pathogenesis has not been supported by results obtained from high-fat feeding of iPLA2β-null (PKO) mice. Unlike livers of human NAFLD and genetically obese rodents, fatty liver induced by high-fat diet is not associated with depletion of hepatic phospholipids. We therefore tested whether iPLA2β could regulate obesity and hepatic steatosis in leptin-deficient mice by cross-breeding PKO with ob/ob mice to generate ob/ob-PKO mice. Here we observed an improvement in ob/ob-PKO mice with significant reduction in serum enzymes, lipids, glucose, insulin as well as improved glucose tolerance, and reduction in islet hyperplasia. The improvement in hepatic steatosis measured by liver triglycerides, fatty acids and cholesterol esters was associated with decreased expression of PPARγ and de novo lipogenesis genes, and the reversal of β-oxidation gene expression. Notably, ob/ob livers contained depleted levels of lysophospholipids and phospholipids, and iPLA2β deficiency in ob/ob-PKO livers lowers the former, but replenished the latter particularly phosphatidylethanolamine (PE) and phosphatidylcholine (PC) that contained arachidonic (AA) and docosahexaenoic (DHA) acids. Compared with WT livers, PKO livers also contained increased PE and PC containing AA and DHA. Thus, iPLA2β deficiency protected against obesity and ob/ob fatty liver which was associated with hepatic fatty-acyl phospholipid remodeling. Our results support the deleterious role of iPLA2β in severe obesity associated NAFLD. PMID:26873633

  16. Upper air teleconnections to Ob River flows and tree rings

    NASA Astrophysics Data System (ADS)

    Meko, David; Panyushkina, Irina; Agafonov, Leonid

    2015-04-01

    The Ob River, one of the world's greatest rivers, with a catchment basin about the size of Western Europe, contributes 12% or more of the annual freshwater inflow to the Arctic Ocean. The input of heat and fresh water is important to the global climate system through effects on sea ice, salinity, and the thermohaline circulation of the ocean. As part of a tree-ring project to obtain multi-century long information on variability of Ob River flows, a network of 18 sites of Pinus, Larix, Populus and Salix has been collected along the Ob in the summers of 2013 and 2014. Analysis of collections processed so far indicates a significant relationship of tree-growth to river discharge. Moderation of the floodplain air temperature regime by flooding appears to be an important driver of the tree-ring response. In unraveling the relationship of tree-growth to river flows, it is important to identify atmospheric circulation features directly linked to observed time series variations of flow and tree growth. In this study we examine statistical links between primary teleconnection modes of Northern Hemisphere upper-air (500 mb) circulation, Ob River flow, and tree-ring chronologies. Annual discharge at the mouth of the Ob River is found to be significantly positively related to the phase of the East Atlantic (EA) pattern, the second prominent mode of low-frequency variability over the North Atlantic. The EA pattern, consisting of a north-south dipole of pressure-anomaly centers spanning the North Atlantic from east to west, is associated with a low-pressure anomaly centered over the Ob River Basin, and with a pattern of positive precipitation anomaly of the same region. The positive correlation of discharge and EA is consistent with these know patterns, and is contrasted with generally negative (though smaller) correlations between EA and tree-ring chronologies. The signs of correlations are consistent with a conceptual model of river influence on tree growth through air

  17. Leptin Reduces the Expression and Increases the Phosphorylation of the Negative Regulators of GLUT4 Traffic TBC1D1 and TBC1D4 in Muscle of ob/ob Mice

    PubMed Central

    Sáinz, Neira; Rodríguez, Amaia; Catalán, Victoria; Becerril, Sara; Ramírez, Beatriz; Lancha, Andoni; Burgos-Ramos, Emma; Gómez-Ambrosi, Javier; Frühbeck, Gema

    2012-01-01

    Leptin improves insulin sensitivity in skeletal muscle. Our goal was to determine whether proteins controlling GLUT4 traffic are altered by leptin deficiency and in vivo leptin administration in skeletal muscle of wild type and ob/ob mice. Leptin-deficient ob/ob mice were divided in three groups: control, leptin-treated (1 mg/kg/d) and leptin pair-fed ob/ob mice. Microarray analysis revealed that 1,546 and 1,127 genes were regulated by leptin deficiency and leptin treatment, respectively. Among these, we identified 24 genes involved in intracellular vesicle-mediated transport in ob/ob mice. TBC1 domain family, member 1 (Tbc1d1), a negative regulator of GLUT4 translocation, was up-regulated (P = 0.001) in ob/ob mice as compared to wild types. Importantly, leptin treatment reduced the transcript levels of Tbc1d1 (P<0.001) and Tbc1d4 (P = 0.004) in the leptin-treated ob/ob as compared to pair-fed ob/ob animals. In addition, phosphorylation levels of TBC1D1 and TBC1D4 were enhanced in leptin-treated ob/ob as compared to control ob/ob (P = 0.015 and P = 0.023, respectively) and pair-fed ob/ob (P = 0.036 and P = 0.034, respectively) mice. Despite similar GLUT4 protein expression in wild type and ob/ob groups a different immunolocalization of this protein was evidenced in muscle sections. Leptin treatment increased GLUT4 immunoreactivity in gastrocnemius and extensor digitorum longus sections of leptin-treated ob/ob mice. Moreover, GLUT4 protein detected in immunoprecipitates from TBC1D4 was reduced by leptin replacement compared to control ob/ob (P = 0.013) and pair-fed ob/ob (P = 0.037) mice. Our findings suggest that leptin enhances the intracellular GLUT4 transport in skeletal muscle of ob/ob animals by reducing the expression and activity of the negative regulators of GLUT4 traffic TBC1D1 and TBC1D4. PMID:22253718

  18. Biodegradation of trichloroethylene by Methylosinus trichosporium OB3b.

    PubMed Central

    Tsien, H C; Brusseau, G A; Hanson, R S; Waclett, L P

    1989-01-01

    The methanotroph Methylosinus trichosporium OB3b, a type II methanotroph, degraded trichloroethylene at rates exceeding 1.2 mmol/h per g (dry weight) following the appearance of soluble methane monooxygenase in continuous and batch cultures. Cells capable oxidizing trichloroethylene contained components of soluble methane monooxygenase as demonstrated by Western blot (immunoblot) analysis with antibodies prepared against the purified enzyme. Growth of cultures in a medium containing 0.25 microM or less copper sulfate caused derepression of the synthesis of soluble methane monooxygenase. In these cultures, the specific rates of methane and methanol oxidation did not change during growth, while trichloroethylene oxidation increased with the appearance of soluble methane monooxygenase. M. trichosporium OB3b cells that contained soluble methane monooxygenase also degraded vinyl chloride, 1,1-dichloroethylene, cis-1,2-dichloroethylene, and trans-1,2-dichloroethylene. Images PMID:2515801

  19. An infrared supershell surrounding the Cygnus OB1 association

    NASA Technical Reports Server (NTRS)

    Saken, Jon M.; Shull, J. M.; Garmany, Catharine D.; Nichols-Bohlin, Joy; Fesen, Robert A.

    1992-01-01

    New studies are reported of a large, 2 x 5 deg peanut-shaped cavity in the far-infrared emission seen using IRAS data for the Cygnus X region. A more complete and better defined infrared supershell than reported by Lozinskaya and Repin (1990) is found and connected to the Cyg OB1 association. It is shown that the cavity represents the early stages of a superbubble produced by the winds and possible SNe from 10 to 20 massive stars. The locations and properties of these stars are used to estimate the energy deposition rate and to understand the manner in which supershells form and propagate. In Cyg OB1, spatially distributed subclustering appears to have played an important role in determining the nonspherical morphology of the superbubble.

  20. PHOTOEVAPORATING PROPLYD-LIKE OBJECTS IN CYGNUS OB2

    SciTech Connect

    Wright, Nicholas J.; Drake, Jeremy J.; Guarcello, Mario G.; Hora, Joseph L.; Drew, Janet E.; Gutermuth, Robert A.; Kraemer, Kathleen E.

    2012-02-20

    We report the discovery of 10 proplyd-like objects in the vicinity of the massive OB association Cygnus OB2. They were discovered in IPHAS H{alpha} images and are clearly resolved in broadband Hubble Space Telescope/Advanced Camera for Surveys, near-IR, and Spitzer mid-IR images. All exhibit the familiar tadpole shape seen in photoevaporating objects such as the Orion proplyds, with a bright ionization front at the head facing the central cluster of massive stars and a tail stretching in the opposite direction. Many also show secondary ionization fronts, complex tail morphologies, or multiple heads. We consider the evidence that these are either proplyds or 'evaporating gaseous globules' (EGGs) left over from a fragmenting molecular cloud, but find that neither scenario fully explains the observations. Typical sizes are 50,000-100,000 AU, larger than the Orion proplyds, but in agreement with the theoretical scaling of proplyd size with distance from the ionizing source. These objects are located at projected separations of {approx}6-14 pc from the OB association, compared to {approx}0.1 pc for the Orion proplyds, but are clearly being photoionized by the {approx}65 O-type stars in Cyg OB2. Central star candidates are identified in near- and mid-IR images, supporting the proplyd scenario, though their large sizes and notable asymmetries are more consistent with the EGG scenario. A third possibility is therefore considered that these are a unique class of photoevaporating partially embedded young stellar objects that have survived the destruction of their natal molecular cloud. This has implications for the properties of stars that form in the vicinity of massive stars.

  1. Flooding of the Ob and Irtysh Rivers, Russia

    NASA Technical Reports Server (NTRS)

    2002-01-01

    These images from the Moderate Resolution Imaging Spectroradiometer (MODIS) on the Terra satellite shows the cause and effect of the large-scale seasonal flooding experienced on rivers throughout Siberia each year. Because many Siberian rivers flow from south to north, they flood regularly in the spring as meltwater from southern latitudes backs up against the still-frozen northern reaches of the rivers.These images show the Ob' River on the western edge of the Central Siberian Plateau. The images from June 20, 2002, show the mouth of the Ob' River (large river at left) where it empties into Kara Sea. In the false-color image, Vegetation appears in bright green, water appears dark blue or black, and ice appears bright blue. The ice is still choking the river's outlet to the sea.The effect of this ice block on the more southern stretches of the river can be seen in the images captured on June 17. In the false-color image, water is black, vegetation is in shades of gold and green, and clouds are pale orange. In the northernmost portion of the Ob' visible in this image (the Ob' runs southeast to northwest in the image), what is normally a fine mesh of braided streams and branches of the river channel has become almost a lake in places. The flood waters have engorged the river to 52 kilometers (32 miles) wide in places. Rivers can back up for hundreds of miles, and cause devastating flooding for towns and villages along the banks. Often, explosives are dropped into ice jams in an effort to free the river and give the flood waters a chance to escape. The spring and summer floods of 2002 have proven to be quite severe and perhaps as many as 100,000 people have been affected across the country. Credit: Jacques Descloitres, MODIS Land Rapid Response Team, NASA/GSFC

  2. Photoevaporating Proplyd-like Objects in Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas J.; Drake, Jeremy J.; Drew, Janet E.; Guarcello, Mario G.; Gutermuth, Robert A.; Hora, Joseph L.; Kraemer, Kathleen E.

    2012-02-01

    We report the discovery of 10 proplyd-like objects in the vicinity of the massive OB association Cygnus OB2. They were discovered in IPHAS Hα images and are clearly resolved in broadband Hubble Space Telescope/Advanced Camera for Surveys, near-IR, and Spitzer mid-IR images. All exhibit the familiar tadpole shape seen in photoevaporating objects such as the Orion proplyds, with a bright ionization front at the head facing the central cluster of massive stars and a tail stretching in the opposite direction. Many also show secondary ionization fronts, complex tail morphologies, or multiple heads. We consider the evidence that these are either proplyds or "evaporating gaseous globules" (EGGs) left over from a fragmenting molecular cloud, but find that neither scenario fully explains the observations. Typical sizes are 50,000-100,000 AU, larger than the Orion proplyds, but in agreement with the theoretical scaling of proplyd size with distance from the ionizing source. These objects are located at projected separations of ~6-14 pc from the OB association, compared to ~0.1 pc for the Orion proplyds, but are clearly being photoionized by the ~65 O-type stars in Cyg OB2. Central star candidates are identified in near- and mid-IR images, supporting the proplyd scenario, though their large sizes and notable asymmetries are more consistent with the EGG scenario. A third possibility is therefore considered that these are a unique class of photoevaporating partially embedded young stellar objects that have survived the destruction of their natal molecular cloud. This has implications for the properties of stars that form in the vicinity of massive stars.

  3. FIVE MORE MASSIVE BINARIES IN THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Kiminki, Daniel C.; Kobulnicky, Henry A.; Gilbert, Ian; Bird, Sarah; Chunev, Georgi

    2009-06-15

    We present the orbital solutions for four OB spectroscopic binaries, MT145, GSC 03161 - 00815, 2MASS J20294666+4105083, and Schulte 73, and the partial orbital solution to the B spectroscopic binary, MT372, as part of an ongoing study to determine the distribution of orbital parameters for massive binaries in the Cygnus OB2 Association. MT145 is a new, single-lined, moderately eccentric (e = 0.291 {+-} 0.009) spectroscopic binary with period of 25.140 {+-} 0.008 days. GSC 03161 - 00815 is a slightly eccentric (e = 0.10 {+-} 0.01), eclipsing, interacting and double-lined spectroscopic binary with a period of 4.674 {+-} 0.004 days. 2MASS J20294666+4105083 is a moderately eccentric (e = 0.273 {+-} 0.002) double-lined spectroscopic binary with a period of 2.884 {+-} 0.001 days. Schulte 73 is a slightly eccentric (e = 0.169 {+-} 0.009), double-lined spectroscopic binary with a period of 17.28 {+-} 0.03 days and the first 'twin' in our survey with a mass ratio of q = 0.99 {+-} 0.02. MT372 is a single-lined, eclipsing system with a period of 2.228 days and low eccentricity (e {approx} 0). Of the now 18 known OB binaries in Cyg OB2, 14 have periods and mass ratios. Emerging evidence also shows that the distribution of log(P) is flat and consistent with 'Oepik's Law'.

  4. Development of an anorthositic lunar regolith simulant: OB-1

    NASA Astrophysics Data System (ADS)

    Battler, Melissa Marie

    With the expected return of astronauts to the Moon before 2020 and construction of a permanent lunar base, there is a renewed need to understand the lunar surface environment. It is necessary to determine optimal methods for operations in the lunar regolith at selected landing sites. Regolith is the unconsolidated, abrasive material blanketing the lunar surface (up to ˜15 m thick), and will be the point of contact for humans and infrastructure. It is unlike any material naturally occurring on Earth. This thesis describes the development of the anorthositic physical/mechanical lunar regolith simulant "OB-I", designed for testing robotic lunar drilling and excavation equipment. The simulant comprises terrestrial anorthosite and slag, which have been comminuted and sized with jaw, roll, and ring crushers. OB-1 has been characterized using optical and electron microscopy, X-ray fluorescence spectroscopy, and various particle sizing methods. The resultant grain shapes, size distributions, and mineralogies are comparable to Apollo 16 regolith samples, making the OB-1 simulant appropriate for validating robotic lunar equipment on Earth.

  5. A Sample of OB Stars that Formed in the Field

    NASA Astrophysics Data System (ADS)

    Oey, M. S.; Lamb, J. B.; Kushner, C. T.; Pellegrini, E. W.; Graus, A. S.

    2013-05-01

    We present a sample of 14 OB stars in the Small Magellanic Cloud that meet strong criteria for having formed under extremely sparse star-forming conditions in the field. These stars are a minimum of 28 pc in projection from other OB stars, and they are centered within symmetric, round H II regions. They show no evidence of bow shocks, implying that the targets are not transverse runaway stars. Their radial velocities relative to local H I also indicate that they are not line-of-sight runaway stars. A friends-of-friends analysis shows that nine of the objects present a few low-mass companion stars, with typical mass ratios for the two highest-mass stars of around 0.1. This further substantiates that these OB stars formed in place, and that they can and do form in extremely sparse conditions. This poses strong constraints on theories of star formation and challenges proposed relations between cluster mass and maximum stellar mass.

  6. A SAMPLE OF OB STARS THAT FORMED IN THE FIELD

    SciTech Connect

    Oey, M. S.; Lamb, J. B.; Kushner, C. T.; Pellegrini, E. W.; Graus, A. S.

    2013-05-01

    We present a sample of 14 OB stars in the Small Magellanic Cloud that meet strong criteria for having formed under extremely sparse star-forming conditions in the field. These stars are a minimum of 28 pc in projection from other OB stars, and they are centered within symmetric, round H II regions. They show no evidence of bow shocks, implying that the targets are not transverse runaway stars. Their radial velocities relative to local H I also indicate that they are not line-of-sight runaway stars. A friends-of-friends analysis shows that nine of the objects present a few low-mass companion stars, with typical mass ratios for the two highest-mass stars of around 0.1. This further substantiates that these OB stars formed in place, and that they can and do form in extremely sparse conditions. This poses strong constraints on theories of star formation and challenges proposed relations between cluster mass and maximum stellar mass.

  7. Cygnus OB2 DANCe: A high-precision proper motion study of the Cygnus OB2 association

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas J.; Bouy, Herve; Drew, Janet E.; Sarro, Luis Manuel; Bertin, Emmanuel; Cuillandre, Jean-Charles; Barrado, David

    2016-08-01

    We present a high-precision proper motion study of 873 X-ray and spectroscopically selected stars in the massive OB association Cygnus OB2 as part of the DANCe project. These were calculated from images spanning a 15 yr baseline and have typical precisions <1 mas yr-1. We calculate the velocity dispersion in the two axes to be σ _α (c) = 13.0^{+0.8}_{-0.7} and σ _δ (c) = 9.1^{+0.5}_{-0.5} km s-1, using a two-component, two-dimensional model that takes into account the uncertainties on the measurements. This gives a three-dimensional velocity dispersion of σ3D = 17.8 ± 0.6 km s-1 implying a virial mass significantly larger than the observed stellar mass, confirming that the association is gravitationally unbound. The association appears to be dynamically unevolved, as evidenced by considerable kinematic substructure, non-isotropic velocity dispersions and a lack of energy equipartition. The proper motions show no evidence for a global expansion pattern, with approximately the same amount of kinetic energy in expansion as there is in contraction, which argues against the association being an expanded star cluster disrupted by process such as residual gas expulsion or tidal heating. The kinematic substructures, which appear to be close to virial equilibrium and have typical masses of 40-400 M⊙, also do not appear to have been affected by the expulsion of the residual gas. We conclude that Cyg OB2 was most likely born highly substructured and globally unbound, with the individual subgroups born in (or close to) virial equilibrium, and that the OB association has not experienced significant dynamical evolution since then.

  8. Cygnus OB2 DANCe: A high-precision proper motion study of the Cygnus OB2 association

    NASA Astrophysics Data System (ADS)

    Wright, Nicholas J.; Bouy, Herve; Drew, Janet E.; Sarro, Luis Manuel; Bertin, Emmanuel; Cuillandre, Jean-Charles; Barrado, David

    2016-08-01

    We present a high-precision proper motion study of 873 X-ray and spectroscopically selected stars in the massive OB association Cygnus OB2 as part of the DANCe project. These were calculated from images spanning a 15 year baseline and have typical precisions < 1 mas/yr. We calculate the velocity dispersion in the two axes to be $\\sigma_\\alpha(c) = 13.0^{+0.8}_{-0.7}$ and $\\sigma_\\delta(c) = 9.1^{+0.5}_{-0.5}$ km/s, using a 2-component, 2-dimensional model that takes into account the uncertainties on the measurements. This gives a 3-dimensional velocity dispersion of $\\sigma_{3D} = 17.8 \\pm 0.6$ km/s implying a virial mass significantly larger than the observed stellar mass, confirming that the association is gravitationally unbound. The association appears to be dynamically unevolved, as evidenced by considerable kinematic substructure, non-isotropic velocity dispersions and a lack of energy equipartition. The proper motions show no evidence for a global expansion pattern, with approximately the same amount of kinetic energy in expansion as there is in contraction, which argues against the association being an expanded star cluster disrupted by process such as residual gas expulsion or tidal heating. The kinematic substructures, which appear to be close to virial equilibrium and have typical masses of 40-400 M$_\\odot$, also do not appear to have been affected by the expulsion of the residual gas. We conclude that Cyg OB2 was most likely born highly substructured and globally unbound, with the individual subgroups born in (or close to) virial equilibrium, and that the OB association has not experienced significant dynamical evolution since then.

  9. Mogat1 deletion does not ameliorate hepatic steatosis in lipodystrophic (Agpat2-/-) or obese (ob/ob) mice.

    PubMed

    Agarwal, Anil K; Tunison, Katie; Dalal, Jasbir S; Yen, Chi-Liang Eric; Farese, Robert V; Horton, Jay D; Garg, Abhimanyu

    2016-04-01

    Reducing triacylglycerol (TAG) in the liver continues to pose a challenge in states of nonalcoholic hepatic steatosis. MonoacylglycerolO-acyltransferase (MOGAT) enzymes convert monoacylglycerol (MAG) to diacylglycerol, a precursor for TAG synthesis, and are involved in a major pathway of TAG synthesis in selected tissues, such as small intestine. MOGAT1 possesses MGAT activity in in vitro assays, but its physiological function in TAG metabolism is unknown. Recent studies suggest a role for MOGAT1 in hepatic steatosis in lipodystrophic [1-acylglycerol-3-phosphateO-acyltransferase (Agpat)2(-/-)] and obese (ob/ob) mice. To test this, we deletedMogat1in theAgpat2(-/-)andob/obgenetic background to generateMogat1(-/-);Agpat2(-/-)andMogat1(-/-);ob/obdouble knockout (DKO) mice. Here we report that, despite the absence ofMogat1in either DKO mouse model, we did not find any decrease in liver TAG by 16 weeks of age. Additionally, there were no measureable changes in plasma glucose (diabetes) and insulin resistance. Our data indicate a minimal role, if any, of MOGAT1 in liver TAG synthesis, and that TAG synthesis in steatosis associated with lipodystrophy and obesity is independent of MOGAT1. Our findings suggest that MOGAT1 likely has an alternative function in vivo. PMID:26880786

  10. Reduced inflammatory response and increased microcirculatory disturbances during hepatic ischemia-reperfusion injury in steatotic livers of ob/ob mice

    PubMed Central

    Hasegawa, Tadashi; Ito, Yoshiya; Wijeweera, Jayanthika; Liu, Jie; Malle, Ernst; Farhood, Anwar; McCuskey, Robert S.; Jaeschke, Hartmut

    2016-01-01

    Steatosis is a major risk factor for complications after liver surgery. Since neutrophil cytotoxicity is critical for ischemia-reperfusion injury in normal livers, the aim of the present study was to evaluate whether an exaggerated inflammatory response could cause the increased injury in steatotic livers. In C57Bl/6 mice, 60 min of warm hepatic ischemia triggered a gradual increase in hepatic neutrophil accumulation during reperfusion with peak levels of 100-fold over baseline at 12 h of reperfusion. Neutrophil extravasation and a specific neutrophil-induced oxidant stress (immunostaining for hypochlorous acid-modified epitopes) started at 6 h of reperfusion and peaked at 12–24 h. Ob/ob mice, which had a severe macrovesicular steatosis, suffered significantly higher injury (alanine transaminase activity: 18,000 ± 2,100 U/l; 65% necrosis) compared with lean littermates (alanine transaminase activity: 4,900 ± 720 U/l; 24% necrosis) at 6 h of reperfusion. However, 62% fewer neutrophils accumulated in steatotic livers. This correlated with an attenuated increase in mRNA levels of several proinflammatory genes in ob/ob mice during reperfusion. In contrast, sham-operated ob/ob mice had a 50% reduction in liver blood flow and 35% fewer functional sinusoids compared with lean littermates. These deficiencies in liver blood flow and the microcirculation were further aggravated only in ob/ob mice during reperfusion. The attenuated inflammatory response and reduced neutrophil-induced oxidant stress observed in steatotic livers during reperfusion cannot be responsible for the dramatically increased injury in ob/ob mice. In contrast, the aggravated injury appears to be mediated by ischemic necrosis due to massive impairment of blood and oxygen supply in the steatotic livers. PMID:17307725

  11. Reduced inflammatory response and increased microcirculatory disturbances during hepatic ischemia-reperfusion injury in steatotic livers of ob/ob mice.

    PubMed

    Hasegawa, Tadashi; Ito, Yoshiya; Wijeweera, Jayanthika; Liu, Jie; Malle, Ernst; Farhood, Anwar; McCuskey, Robert S; Jaeschke, Hartmut

    2007-05-01

    Steatosis is a major risk factor for complications after liver surgery. Since neutrophil cytotoxicity is critical for ischemia-reperfusion injury in normal livers, the aim of the present study was to evaluate whether an exaggerated inflammatory response could cause the increased injury in steatotic livers. In C57Bl/6 mice, 60 min of warm hepatic ischemia triggered a gradual increase in hepatic neutrophil accumulation during reperfusion with peak levels of 100-fold over baseline at 12 h of reperfusion. Neutrophil extravasation and a specific neutrophil-induced oxidant stress (immunostaining for hypochlorous acid-modified epitopes) started at 6 h of reperfusion and peaked at 12-24 h. Ob/ob mice, which had a severe macrovesicular steatosis, suffered significantly higher injury (alanine transaminase activity: 18,000 +/- 2,100 U/l; 65% necrosis) compared with lean littermates (alanine transaminase activity: 4,900 +/- 720 U/l; 24% necrosis) at 6 h of reperfusion. However, 62% fewer neutrophils accumulated in steatotic livers. This correlated with an attenuated increase in mRNA levels of several proinflammatory genes in ob/ob mice during reperfusion. In contrast, sham-operated ob/ob mice had a 50% reduction in liver blood flow and 35% fewer functional sinusoids compared with lean littermates. These deficiencies in liver blood flow and the microcirculation were further aggravated only in ob/ob mice during reperfusion. The attenuated inflammatory response and reduced neutrophil-induced oxidant stress observed in steatotic livers during reperfusion cannot be responsible for the dramatically increased injury in ob/ob mice. In contrast, the aggravated injury appears to be mediated by ischemic necrosis due to massive impairment of blood and oxygen supply in the steatotic livers. PMID:17307725

  12. Hepatic Macrosteatosis Is Partially Converted to Microsteatosis by Melatonin Supplementation in ob/ob Mice Non-Alcoholic Fatty Liver Disease

    PubMed Central

    Lavazza, Antonio; Golic, Igor; Aleksic, Marija; Korac, Aleksandra; Rodella, Luigi Fabrizio; Rezzani, Rita

    2016-01-01

    Background Obesity is a common risk factor for non-alcoholic fatty liver disease (NAFLD). Currently, there are no specific treatments against NAFLD. Thus, examining any molecule with potential benefits against this condition emerged melatonin as a molecule that influences metabolic dysfunctions. The aim of this study was to determine whether melatonin would function against NAFDL, studying morphological, ultrastuctural and metabolic markers that characterize the liver of ob/ob mice. Methods Lean and ob/ob mice were supplemented with melatonin in the drinking water for 8 weeks. Histology and stereology were performed to assess hepatic steatosis and glycogen deposition. Ultrastructural features of mitochondria, endoplasmic reticulum (ER) and their juxtapositions were evaluated in livers of all experimental groups. Furthermore, hepatic distribution and expression of markers of ER and mitochondria (calnexin, ATP sintase β, GRP78 and CHOP) and metabolic dysfunction (RPB4, β-catenin) and cellular longevity (SIRT1) were analyzed. Results Melatonin significantly reduced glycemia, identified also by a decrease of hepatic RBP4 expression, reversed macrosteatosis in microsteatosis at the hepatic pericentral zone, enlarged ER-mitochondrial distance and ameliorated the morphology and organization of these organelles in ob/ob mouse liver. Furthermore, in ob/ob mice, calnexin and ATP synthase β were partially restored, GRP78 and CHOP decreased in periportal and midzonal hepatocytes and β-catenin expression was, in part, restored in peripheral membranes of hepatocytes. Melatonin supplementation to ob/ob mice improves hepatic morphological, ultrastructural and metabolic damage that occurs as a result of NAFLD. Conclusions Melatonin may be a potential adjuvant treatment to limit NAFLD and its progression into irreversible complications. PMID:26824477

  13. Leptin deficient ob/ob mice and diet-induced obese mice responded differently to Roux-en-Y bypass surgery

    PubMed Central

    Hao, Z; Münzberg, H; Rezai-Zadeh, K; Keenan, M; Coulon, D; Lu, H; Berthoud, H-R; Ye, J

    2015-01-01

    OBJECTIVE Weight regain contributes to the therapeutic failure in 15–20% of type 2 diabetic patients after Roux-en-Y gastric bypass surgery (RYGB), and the mechanism remains largely unknown. This study was conducted to explore the mechanism of weight regain. RESEARCH DESIGN Wild-type (WT) diet-induced obese (DIO) mice were used to mimic human obesity, and ob/ob mice were used for leptin deficiency-induced obesity. Two groups of mice were compared in weight regain for 10 months after RYGB. Weight loss, food intake, fecal energy loss and energy expenditure were monitored in the study of weight regain. Fasting insulin, insulin tolerance and homeostatic model assessment-insulin resistance were tested for insulin sensitivity under the weight regain. Weight loss from RYGB and calorie restriction was compared for the impact in insulin sensitivity. RESULTS In WT mice, RYGB induced a sustained weight loss and insulin sensitization over the sham operation in this 10-month study. However, RYGB failed to generate the same effects in leptin-deficient ob/ob mice, which suffered a weight regain over the pre-surgery level. In ob/ob mice, body weight was reduced by RYGB transiently in the first week, recovered in the second week and increased over the baseline thereafter. Weight loss was induced by RYGB relative to that of sham mice, but the loss was not sufficient to keep body weight below the pre-surgery levels. In addition, insulin sensitivity was not improved by the weight loss. The response to RYGB was improved in ob/ob mice by 2 weeks of leptin treatment. Weight loss from calorie restriction had an equivalent effect on insulin sensitization compared with that of RYGB. CONCLUSION Those data demonstrate that ob/ob mice and DIO mice responded differently to RYGB surgery, suggesting that leptin may be one of the factors required for RYGB to prevent weight regain and diabetes recurrence. PMID:25349056

  14. Pathology of the liver in obese and diabetic ob/ob and db/db mice fed a standard or high-calorie diet.

    PubMed

    Trak-Smayra, Viviane; Paradis, Valérie; Massart, Julie; Nasser, Selim; Jebara, Victor; Fromenty, Bernard

    2011-12-01

    Non-alcoholic fatty liver disease (NAFLD) is one of the commonest liver diseases in Western countries. Although leptin deficient ob/ob and db/db mice are frequently used as murine models of NAFLD, an exhaustive characterization of their hepatic lesions has not been reported to date, particularly under calorie overconsumption. Thus, liver lesions were characterized in 78 ob/ob and db/db mice fed either a standard or high-calorie (HC) diet, for one or three months. Steatosis, necroinflammation, apoptosis and fibrosis were assessed and the NAFLD activity score (NAS) was calculated. Steatosis was milder in db/db mice compared to ob/ob mice and was more frequently microvesicular. Although necroinflammation was usually mild in both genotypes, it was aggravated in db/db mice after one month of calorie overconsumption. Apoptosis was observed in db/db mice whereas it was only detected in ob/ob mice after HC feeding. Increased apoptosis was frequently associated with microvesicular steatosis. In db/db mice fed the HC diet for three months, fibrosis was aggravated while steatosis, necroinflammation and apoptosis tended to alleviate. This was associated with increased plasma β-hydroxybutyrate suggesting an adaptive stimulation of hepatic mitochondrial fatty acid oxidation (FAO). Nevertheless, one-third of these db/db mice had steatohepatitis (NAS ≥ 5), whereas none of the ob/ob mice developed non-alcoholic steatohepatitis under the same conditions. Steatosis, necroinflammation, apoptosis and fibrosis are modulated by calorie overconsumption in the context of leptin deficiency. Association between apoptosis and microvesicular steatosis in obese mice suggests common mitochondrial abnormalities. Enhanced hepatic FAO in db/db mice is associated with fibrosis aggravation. PMID:22118645

  15. OBS FOMAR POOL: Gibraltar and ALERTES-RIM experiments.

    NASA Astrophysics Data System (ADS)

    Pazos, Antonio; Martín Davila, Jose; Buforn, Elisa; Cabieces, Roberto; Santos, Jose; Sandoval, Nicolas; Roca, Antoni; Dahm, Torsten

    2016-04-01

    The Eurasian-African plate boundary crosses the called "Ibero-Maghrebian" region from the San Vicente Cape (SW Portugal) to Tunisia including the south Iberia, Alboran Sea, and northern of Morocco and Algeria. The low convergence rate at this plate boundary produces a continuous moderate seismic activity of low magnitude and shallow depth, where the occurrence of large earthquakes is separated by long time intervals, even with associated tsunamis, like the 1755 Lisbon earthquake. In this region, there are also intermediate and very deep earthquakes. Due to the fact that part of the seismic activity is located at marine areas, and also because of the poor geographic azimuthal coverage at some zones provided by the land stations (specially in the SW of the San Vicente Cape area), Royal Spanish Navy Observatory (ROA) acquired three "LOSTERN" broad band (CMG-40T sensors) OBS, manufactured by KUM (Kiel, Germany), and, more recently (2014), the Complutense University of Madrid (UCM) acquired another three with Trillium 120 sensors. All of them conform the OBS FOMAR pool. Since January to November 2014, the FOMAR pool has been deployed along the Gibraltar strait (Gibraltar experiment), in collaboration with SECEGSA (Spanish society to study the fix communication through the Gibraltar Strait), to study the local microseismicity in the Gibraltar strait area. Also, since September 2015, the FOMAR pool has been deployed for 8 months in SW of the San Vicente Cape with an hexagonal array configuration as a part of ALERTES-RIM project. In this work the some preliminary results of the Gibraltar strait and ALERTES-RIM OBS experiment are shown.

  16. Development of Hydrophone and Its Aapplications Use in OBS

    NASA Astrophysics Data System (ADS)

    Lin, C. R.; Kuo, B. Y.; Wang, C. C.; Chang, H. K.; Jang, J. P.

    2014-12-01

    The seismic wave is translated to T-wave when earthquakes occur below seafloor with a traveling speed of 1500 m/s in the SOFAR channel which centers roughly at 1000 m water depths (Tolstoy and Ewing, 1950). Many studies have shown that hydrophone can be set up in the SOFAR channel to collect acoustic data to investigate plate movement (Fox and Dziak, 1999), seismisity (Fox et.al, 2001), characteristic of T-wave (Park et.al, 2001), seafloor volcanism (Nicolas, 1989), among others. Institute of Earth Sciences of Academia Sinica has been collaborating with Taiwan Ocean Research Institute (TORI) and the Institute of Undersea Technology (IUT) of the National Sun Yat-sen University (NSYSU) to succefully build a new wide-band OBS (Yardbird). We hope that Yardbird is not only an OBS but also a multi-function underwater recording plotform. Therefor we are developing a low-power consumption, inexpensive hydrophone to catch acoustic signals. The protoypte of it has passed a series of tests and got some seismic seafloor data. This paper describes the design goal of hydrophone, component specifications, field data analyzed, and discusses the future directions of instrument development. Keywords: T-wave; Hydrophone; OBS; acoustic signal Referance: 1 Fox, C. G. and R. P. Dziak, Intenal deformation of the Gorda Plate observedb y hydroacoustimc onitoring, J . Geophys Res., 104, 17603-17615, 1999. 2 Fox, C. G., H. Matsumoto and T.K.Lau, Monitoring Pacific Ocean seismicity from an autonomous hydrophone array. , J . Geophys. Res., 106,4183-4206, 2001. 3 Nicolas, A., Structures of ophiolites and dynamics of oceanic lithosphere. Dordrecht ; Boston : Kluwer Academic Publishers, 1989. 4. Park, M., R.I. Odom, and D.J. Soukup (2001). Modal scattering: a key to understanding oceanic T-waves, Geophys. Res. Lett. 28, 3401-3404. 5. Tolstoy, I. and M. Ewing (1950). The T phase of shallow-focus earthquakes, Bull. Seismol. Soc. Am. 40, 25-51.

  17. Distributed vs. Clustered Star Formation in Mon OB1 - NGC 2264

    NASA Astrophysics Data System (ADS)

    Fazio, Giovanni; Pipher, Judy; Allen, Lori; Gutermuth, Robert; Megeath, S. Thomas; Myers, Phillip

    2007-05-01

    Following successful IRAC + MIPS observations (75% complete) of the GMCs Cep OB3 and Mon R2 harboring high mass star formation in PID 20403 'Distributed vs. Clustered Star Formation', we propose in this cycle to obtain similar observations for 2 more GMCs, namely Mon OB1 and Cam OB1. The present GTO proposal begins this program by covering part of the distributed area around NGC 2264 in Mon OB1. The rest of Mon OB1 as well as Cam OB1 will be proposed as a GO program. We hope to establish the physical conditions leading to clustered vs. distributed star formation. We will compare results for all these regions against similar studies in clouds harboring low mass star formation, for example, the c2d survey.

  18. FUSE Analysis of the Cyg OB1 Superbubble

    NASA Technical Reports Server (NTRS)

    Nichols, J.; Mendygral, P.

    1996-01-01

    FUSE observations of four stars in the line of sight to the Cyg OB1 IR-detected superbubble have been analyzed for high-velocity features in the O VI interstellar lines, which might be attributed to the shock structure of the superbubble. Multiple components were detected in the spectra of all four stars, with a velocity range of -85 kilometers per second to +29 kilometers per second. As many as four separate velocity components were identified in each spectrum, implying multiple shock structures in the superbubble. Derived column densities of the O VI components indicate shock velocities of 160-190 kilometers per second according to steady state shock theory.

  19. The object SNO 74 in the association Ara OB 1

    NASA Astrophysics Data System (ADS)

    Gyulbudaghian, A. L.

    2013-06-01

    The star formation region SNO 74 embedded in a dark cloud in the association Ara OB 1 is studied. This object is coupled to a compact HII region visible in the IR and to a close cluster of IR stars. 12CO(1-0) observations reveal the existence of red and blue molecular outflows; i.e., this is a bipolar outflow. An image taken with the SIMBA detector at 1.2 mm contains a second active region and a bridge lying to the W of the first active region and accompanied by strong absorption. The red molecular outflow is directed toward this second active region and the bridge.

  20. Reduction of obesity, as induced by leptin, reverses endothelial dysfunction in obese (Lep(ob)) mice

    NASA Technical Reports Server (NTRS)

    Winters, B.; Mo, Z.; Brooks-Asplund, E.; Kim, S.; Shoukas, A.; Li, D.; Nyhan, D.; Berkowitz, D. E.

    2000-01-01

    Obesity is a major health care problem and is associated with significant cardiovascular morbidity. Leptin, a neuroendocrine hormone released by adipose tissue, is important in modulating obesity by signaling satiety and increasing metabolism. Moreover, leptin receptors are expressed on vascular endothelial cells (ECs) and mediate angiogenesis. We hypothesized that leptin may also play an important role in vasoregulation. We investigated vasoregulatory mechanisms in the leptin-deficient obese (ob/ob) mouse model and determined the influence of leptin replacement on endothelial-dependent vasorelaxant responses. The direct effect of leptin on EC nitric oxide (NO) production was also tested by using 4, 5-diaminofluorescein-2 diacetate staining and measurement of nitrate and nitrite concentrations. Vasoconstrictor responses to phenylephrine, norepinephrine, and U-46619 were markedly enhanced in aortic rings from ob/ob mice and were modulated by NO synthase inhibition. Vasorelaxant responses to ACh were markedly attenuated in mesenteric microvessels from ob/ob mice. Leptin replacement resulted in significant weight loss and reversal of the impaired endothelial-dependent vasorelaxant responses observed in ob/ob mice. Preincubation of ECs with leptin enhanced the release of NO production. Thus leptin-deficient ob/ob mice demonstrate marked abnormalities in vasoregulation, including impaired endothelial-dependent vasodilation, which is reversed by leptin replacement. These findings may be partially explained by the direct effect of leptin on endothelial NO production. These vascular abnormalities are similar to those observed in obese, diabetic, leptin-resistant humans. The ob/ob mouse may, therefore, be an excellent new model for the study of the cardiovascular effects of obesity.

  1. The effect of leptin replacement on sleep-disordered breathing in the leptin-deficient ob/ob mouse.

    PubMed

    Pho, H; Hernandez, A B; Arias, R S; Leitner, E B; Van Kooten, S; Kirkness, J P; Schneider, H; Smith, P L; Polotsky, V Y; Schwartz, A R

    2016-01-01

    Obese leptin-deficient (ob/ob) mice demonstrate defects in upper airway structural and neuromuscular control. We hypothesized that these defects predispose to upper airway obstruction during sleep, and improve with leptin administration. High-fidelity polysomnographic recordings were conducted to characterize sleep and breathing patterns in conscious, unrestrained ob/ob mice (23 wk, 67.2 ± 4.1 g, n = 13). In a parallel-arm crossover study, we compared responses to subcutaneous leptin (1 μg/h) vs. vehicle on respiratory parameters during NREM and REM sleep. Upper airway obstruction was defined by the presence of inspiratory airflow limitation (IFL), as characterized by an early inspiratory plateau in airflow at a maximum level (V̇Imax) with increasing effort. The severity of upper airway obstruction (V̇Imax) was assessed along with minute ventilation (V̇E), tidal volume (VT), respiratory rate (RR), inspiratory duty cycle, and mean inspiratory flow at each time point. IFL occurred more frequently in REM sleep (37.6 ± 0.2% vs. 1.1 ± 0.0% in NREM sleep, P < 0.001), and leptin did not alter its frequency. V̇Imax (3.7 ± 1.1 vs. 2.7 ± 0.8 ml/s, P < 0.001) and V̇E increased (55.4 ± 22.0 vs. 39.8 ± 16.4 ml/min, P < 0.001) with leptin vs. vehicle administration. The increase in V̇E was due to a significant increase in VT (0.20 ± 0.06 vs. 0.16 ± 0.05 ml, P < 0.01) rather than RR. Increases in V̇E were attributable to increases in mean inspiratory flow (2.5 ± 0.8 vs. 1.8 ± 0.6 ml/s, P < 0.001) rather than inspiratory duty cycle. Similar increases in V̇E and its components were observed in non-flow-limited breaths during NREM and REM sleep. These responses suggest that leptin stabilized pharyngeal patency and increased drive to both the upper airway and diaphragm during sleep. PMID:26472867

  2. The Seismic Broad Band Western Mediterranean (wm) Network and the Obs Fomar Pool: Current state and Obs activities.

    NASA Astrophysics Data System (ADS)

    Pazos, Antonio; Davila, Jose Martin; Buforn, Elisa; Bezzeghoud, Mourad; Harnafi, Mimoun; Mattesini, Mauricio; Caldeira, Bento; Hanka, Winfried; El Moudnib, Lahcen; Strollo, Angelo; Roca, Antoni; Lopez de Mesa, Mireya; Dahm, Torsten; Cabieces, Roberto

    2016-04-01

    The Western Mediterranean (WM) seismic network started in 1996 as an initiative of the Royal Spanish Navy Observatory (ROA) and the Universidad Complutense de Madrid (UCM), with the collaboration of the GeoForschungsZentrum (GFZ) of Potsdam. A first broad band seismic station (SFUC) was installed close to Cádiz (South Spain). Since then, additional stations have been installed in the Ibero-Moghrebian region. In 2005, the "WM" code was assigned by the FDSN and new partners were jointed: Evora University (UEVO, Portugal), the Scientifique Institute of Rabat (ISRABAT, Morocco), and GFZ. Now days, the WM network is composed by 15 BB stations, all of them with Streckaisen STS-2 or STS-2.5 sensors, Quanterra or Earthdata digitizers and SeiscomP. Most them have co-installed a permanent geodetic GPS stations, and some them also have an accelerometer. There are 10 stations deployed in Spanish territory (5 in the Iberian peninsula, 1 in Balearic islands and 4 in North Africa Spanish places) with VSAT or Internet communications, 2 in Portugal (one of them without real time), and 3 in Morocco (2 VSAT and 1 ADSL). Additionally, 2 more stations (one in South Spain and one in Morocco) will be installed along this year. Additionally ROA has deployed a permanent real time VBB (CMG-3T: 360s) station at the Alboran Island. Due to the fact that part of the seismic activity is located at marine areas, and also because of the poor geographic azimuthal coverage at some zones provided by the land stations (specially in the SW of the San Vicente Cape area), ROA and UCM have acquired six broad band "LOBSTERN" OBS, manufactured by KUM (Kiel, Germany), conforming the OBS FOMAR pool. Three of them with CMG-40T sensor and the other with Trillium 120. These OBS were deployed along the Gibraltar strait since January to November 2014 to study the microseismicity in the Gibraltar strait area. In September 2015 FOMAR network has been deployed in SW of the San Vicente Cape for 8 months as a part of

  3. Analysis of methane biodegradation by Methylosinus trichosporium OB3b

    PubMed Central

    Rodrigues, Andréa dos Santos; Salgado, Belkis Valdman e Andréa Medeiros

    2009-01-01

    The microbial oxidation of methane in the atmosphere is performed by methanotrophic bacteria that use methane as a unique source of carbon and energy. The objective of this work consisted of the investigation of the best conditions of methane biodegradation by methanotrophic bacteria Methylosinus trichosporium OB3b that oxidize it to carbon dioxide, and the use of these microorganisms in monitoring methods for methane. The results showed that M. trichosporium OB3b was capable to degrade methane in a more effective way with an initial microorganism concentration of 0.0700 g.L-1, temperature of 30ºC, pH 6.5 and using 1.79 mmol of methane. In these same conditions, there was no bacterial growth when 2.69 mmol of methane was used. The specific rate of microorganism growth, the conversion factor, the efficiency and the volumetric productivity, for the optimized conditions of biodegradation were, respectively, 0.0324 h-1, 0.6830 gcells/gCH4, 73.73% and 2.7732.10-3 gcells/L.h. The final product of methane microbiological degradation, carbon dioxide, was quantified through the use of a commercial electrode, and, through this, the grade of methane conversion in carbon dioxide was calculated. PMID:24031362

  4. A new Shallow Water Cabled OBS System off California

    NASA Astrophysics Data System (ADS)

    Rademacher, Horst; Pearcey, Chris; Mangano, Giorgio; Guralp, Cansun; Pearce, Nathan

    2014-05-01

    During the summer and fall of 2013 we installed a turnkey cabled network of four combination broadband velocity/acceleration ocean bottom sensors (OBS) on the sea floor near Point Buchon in the Northeastern Pacific Ocean off the coast of Central California. We implemented a novel network design by daisy-chaining the instruments to one single multistranded cable. The signals of each station are digitized in-situ and then transmitted via dedicated optical fiber links inside the cable to a shore station. From there they are fed in real time via a cell phone modem into several seismic networks in California. The goal of this dense network is to monitor the microseismicity of two offshore faults running parallel to the strike of the San Andreas Fault. However, because the network is installed in rather shallow water near the coast, the action of waves and swell at the sea surface affect the sensor registrations much stronger as compared to the typical deep water installation of OBS equipment. We will report about the challenges of installing and maintaining the network and present some initial results.

  5. Expression of the extracellular domain of OB-cadherin as an Fc fusion protein using bicistronic retroviral expression vector

    PubMed Central

    Lira, Cristina B. B.; Chu, Khoi; Lee, Yu-Chen; Hu, Mickey C-T.; Lin, Sue-Hwa

    2008-01-01

    Osteoblast cadherin (OB-cadherin, also known as cadherin-11) is a Ca2+-dependent homophilic cell adhesion molecule that is expressed mainly in osteoblasts. OB-cadherin is expressed in prostate cancer and may be involved in the homing of metastatic prostate cancer cells to bone. The extracellular domain of OB-cadherin may be used to inhibit the adhesion between prostate cancer cells and osteoblasts. In this report, we describe the expression of the extracellular domain of OB-cadherin as an Fc fusion protein (OB-CAD-Fc) in human embryonic kidney 293FT cells using a bicistronic retroviral vector. Coexpression of GFP and OB-CAD-Fc through the bicistronic vector permitted enrichment of OB-CAD-Fc–expressing cells by fluorescence-activated cell sorting. Recombinant OB-CAD-Fc proteins were secreted into cell medium, and about 0.85 mg of purified OB-CAD-Fc protein was purified from 1 liter of the conditioned medium using immobilized protein A-affinity chromatography. The purified OB-CAD-Fc was biologically active because it supported the adhesion of PC3 cells and L cells transduced with OB-cadherin. The availability of OB-CAD-Fc offers opportunities to test whether OB-CAD-Fc can be used to inhibit OB-cadherin–mediated prostate cancer bone metastasis in vivo or to generate antibodies for inhibiting the adhesion between prostate cancer cells and osteoblasts. PMID:18620062

  6. The Luminosity Function of OB Associations in the Galaxy

    NASA Astrophysics Data System (ADS)

    McKee, Christopher F.; Williams, Jonathan P.

    1997-02-01

    OB associations ionize the interstellar medium, producing both localized H II regions and diffuse ionized gas. The supernovae resulting from these associations pressurize and stir the interstellar medium. Using Smith, Biermann, & Mezger's compilation of radio H II regions in the Galaxy, and Kennicutt, Edgar, & Hodge's optical study of H II regions in nearby galaxies, we show that the luminosity distribution of giant OB associations in the Galaxy can be fit by a truncated power law of the form \\Nscra(>S)=\\Nscrau[(Su/S)-1], where S is the ionizing photon luminosity, \\Nscra(>S) is the number of associations with a luminosity of at least S, and Su is the upper limit to the distribution. The coefficient \\Nscrau is the number of the most luminous associations, with a luminosity between 0.5Su and Su. For the Galaxy, \\Nscrau=6.1 the fact that the number of the most luminous associations is significantly larger than unity indicates that there is a physical limit to the maximum size of H II regions in the Galaxy. To extend the luminosity distribution to small H II regions, we assume that the birthrate of associations, \\Nscr\\dota(>\\Nscr*), is also a truncated power law, \\Nscr\\dota(>\\Nscr*)~[(\\Nscr*u/\\Nscr*)-1], where \\Nscr* is the number of stars in the association. For large associations, the ionizing luminosity is proportional to the number of stars, S~\\Nscr* for smaller associations, we use both an analytic and a Monte Carlo approach to find the resulting luminosity distribution \\Nscra(>S). H II regions are generally centrally concentrated, with only the dense central regions being bright enough to appear in radio catalogs. Anantharamaiah postulated that radio H II regions have extended envelopes in order to account for diffuse radio recombination line emission in the Galaxy. Some of these envelopes are visible as the ionized ``worms'' discussed by Heiles and coworkers. We estimate that on the average the envelopes of radio H II regions absorb about twice

  7. Refraction Interferometry and Free-surface Multiples for Large-offset OBS Data

    NASA Astrophysics Data System (ADS)

    Schuster, G. T.; Bharadwaj, P.; McIntosh, K.

    2011-12-01

    We present successful results for applying refraction interferometry to ocean bottom seismic (OBS) data. The OBS data were recorded by six inline OBS stations with a 15 km station spacing and a source ship shooting every 150 meters; the maximum source offset from an OBS station is 181 km. Each virtual trace was created by correlating and stacking up to 150 trace pairs to create a super-virtual refraction arrival having a theoretical signal-to-noise ratio (SNR) enhancement of up to 12:1. Free-surface refraction multiples were also utilized to enhance the SNR of primary refractions and virtually double the number of OBS sites from six to twelve stations. Results show the successful reconstruction of far-offset traces out to a source-receiver offset of 120 km. The super-virtual traces increase the number of pickable first arrivals from approximately 1,600 to more than 3,100 for a subset of the OBS data where the source is only on one side of the recording stations. In addition, the head waves associated with the first-order free-surface multiples allow for the creation of six new common receiver gathers (CRGs) recorded at virtual OBS stations, located about 5 km from any of the actual OBS stations. These new traces double the number of OBS stations compared to the original survey and increases the number of pickable traces from 1600 to 6200. In summary, our results with this OBS data set demonstrate that refraction interferometry can more than triple the number of pickable first arrivals in long-offset traces. This suggests the possibility that the number of independent OBS stations can be increased by N-fold if the free-surface refraction multiples up to the Nth-order are of high SNR and if the recording time is appropriately lengthened.

  8. Externally Induced Disks Photoevaporation in the Massive Cluster Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Guarcello, Mario

    2013-07-01

    CygnusOB2, the most massive OB association of the Cygnus-X region, hosts more than 2000 OB stars and a population of young pre-Main Sequence stars. It is 1.4kpc distant from the Sun and the best target to study star formation and disk evolution in the presence of a large number of massive stars. We present a multiwavelength study of protoplanetary disks in Cyg OB2, based on new deep optical and X-ray data obtained with OSIRIS@GTC and Chandra/ACIS-I (the Cygnus OB2 Chandra Legacy Survey), and on archival data from 2MASS, UKIDSS, IPHAS and the Spitzer Legacy Survey of the Cygnus-X Region. We compare the spatial variation of disk fraction with the intensity of the ionized flux emitted by the OB members of CygOB2. We show that the disk fraction decreases gradually with increasing the intensity of the ionizing flux. The results suggests protoplanetary disks in Cyg OB2 suffer long-range photoevaporation induced by the OB star population. This is different than the results obtained in the study of NGC6611 (i.e. Guarcello et al 2007), NGC2264 (i.e. Balog et al. 2007), and the Trapezium in Orion (i.e. Storzer & Hollenbach 1999), where disk fraction drops only in the proximity of the OB stars. We discuss this difference in terms of the larger ionizing flux and older age in Cyg OB2 with respect to these other clusters and touch upon the relevance of this work for planet formation in the universe.

  9. Cosmography of OB stars in the solar neighbourhood

    NASA Astrophysics Data System (ADS)

    Bouy, H.; Alves, J.

    2015-12-01

    We construct a 3D map of the spatial density of OB stars within 500 pc from the Sun using the Hipparcos catalogue and find three large-scale stream-like structures that allow a new view on the solar neighbourhood. The spatial coherence of these blue streams and the monotonic age sequence over hundreds of parsecs suggest that they are made of young stars, similar to the young streams that are conspicuous in nearby spiral galaxies. The three streams are 1) the Scorpius to Canis Majoris stream, covering 350 pc and 65 Myr of star formation history; 2) the Vela stream, encompassing at least 150 pc and 25 Myr of star formation history; and 3) the Orion stream, including not only the well-known Orion OB1abcd associations, but also a large previously unreported foreground stellar group lying only 200 pc from the Sun. The map also reveals a remarkable and previously unknown nearby OB association, between the Orion stream and the Taurus molecular clouds, which might be responsible for the observed structure and star formation activity in this cloud complex. This new association also appears to be the birthplace of Betelgeuse, as indicated by the proximity and velocity of the red giant. If this is confirmed, it would solve the long-standing puzzle of the origin of Betelgeuse. The well-known nearby star-forming low-mass clouds, including the nearby T and R associations Lupus, Cha, Oph, CrA, Taurus, Vela R1, and various low-mass cometary clouds in Vela and Orion, appear in this new view of the local neighbourhood to be secondary star formation episodes that most likely were triggered by the feedback from the massive stars in the streams. We also recover well-known star clusters of various ages that are currently cruising through the solar neighbourhood. Finally, we find no evidence of an elliptical structure such as the Gould belt, a structure we suggest is a 2D projection effect, and not a physical ring. Table 3 is available in elctronic form at http://www.aanda.org

  10. Association between diencephalic thyroliberin and arterial blood pressure in agouti-yellow and ob/ob mice may be mediated by leptin.

    PubMed

    Burgueño, Adriana L; Landa, Maria S; Schuman, Mariano L; Alvarez, Azucena L; Carabelli, Julieta; García, Silvia I; Pirola, Carlos J

    2007-10-01

    Leptin, a hormone secreted by the adipose tissue, stimulates anorexigenic peptides and also inhibits orexigenic peptides in hypothalamic arcuate nuclei-located neurons. It also counteracts the starvation-induced suppression of thyroid hormones by up-regulating the expression of preproTRH gene. On the other hand, in addition to its role as a modulator of the thyroid-hypothalamic-hypophysial axis, thyrotropin-releasing hormone (TRH) acts as a modulator of the cardiovascular system. In fact, we reported that overexpression of diencephalic TRH (dTRH) induces hypertension. We have recently shown that, in rats with obesity-induced hypertension, hyperleptinemia may produce an increase of dTRH together with an elevation of arterial blood pressure (ABP) through an increase of sympathetic activity and that these alterations were reversed by antisense oligonucleotide and small interfering RNA against preproTRH treatments. Here we explore the possible role of dTRH as a mediator involved in leptin-induced hypertension in 2 obesity mouse models: agouti-yellow mice, which are hyperleptinemic and hypertensive, and ob/ob mice, which lack functional circulating leptin. These 2 models share some characteristics, but ob/ob mice show lower ABP and plasma catecholamines levels. Then, for the first time, we report that there is a clear association between ABP and dTRH levels in both mouse models, as we have found that dTRH content was elevated in agouti-yellow mice and diminished in ob/ob mice compared with their controls. We also show that, after 3 days of subcutaneous leptin injections (10 microg/12 hours), ABP and dTRH increased significantly in ob/ob mice with no alterations of thyroid hormone levels. These results add evidence to the putative molecular mechanisms for the strong association between obesity and hypertension. PMID:17884458

  11. Phenotypic effects of an induced mutation of the ObRa isoform of the leptin receptor★

    PubMed Central

    Li, Zhiying; Ceccarini, Giovanni; Eisenstein, Michael; Tan, Keith; Friedman, Jeffrey Michael

    2013-01-01

    Leptin receptors play critical roles in mediating leptin's pleiotropic effects on mammalian physiology. To date, six splice variants of the leptin receptor gene have been identified [1–3]. These splice variants have identical extracellular leptin binding motifs but different intracellular C termini. The finding that mutations specifically ablating the function of ObRb cause obesity has established a critical role for this isoform in leptin signaling [1,7]. ObRa is the most abundant splicing isoform with a broad tissue distribution [5], and it has been proposed to play roles in regulating leptin bioavailability, CSF (cerebrospinal fluid) transport and function by forming heterodimers with ObRb and also activating signal transduction via JAK2 in-vitro [5–10]. To assess the in-vivo role of ObRa, we generated an ObRa KO mouse by deleting the ObRa-specific exon 19a. Homozygous mutant mice breed normally and are indistinguishable from wild-type mice on regular chow diet, but show a slightly increased basal plasma leptin, a slight improvement of their GTT and a slightly reduced response to systemic leptin administration. These mice also show a modest but statistically significant increase in weight when placed on a high fat diet with a slightly reduced CSF/plasma ratio of leptin. These data suggest that ObRa plays a role in mediating some of leptin's effects but that the phenotypic consequences are modest compared to a deletion of ObRb. PMID:24327953

  12. A Behavioral Approach to Improving Self-Care Skills in OBS Patients.

    ERIC Educational Resources Information Center

    McEvoy, Cathy L.; Patterson, Roger L.

    Traditionally, the treatment of geriatric patients suffering from Organic Brain Syndrome (OBS) has been characterized by non-therapeutic custodial care. To determine whether elderly clients with dementia can benefit from self-care skill training, and to compare their progress with clients without OBS, 30 clients of the Residential Aging Program in…

  13. Performance analysis of OVPN over OBS/MPLS architecture with different priorities traffic

    NASA Astrophysics Data System (ADS)

    Ji, Wei; Zhang, Min; Ye, Peida

    2005-11-01

    The implementing of optical virtual private networks (OVPNs) over an optical burst-switching (OBS) network based on MPLS is discussed. The network architecture, the core and access nodes faculty are described. We also propose a new model with three traffic priorities, which used to analysis the delay and blocking performance of OVPN over OBS/MPLS network based on queuing theory.

  14. Terminal velocities of the winds from rapidly rotating OB stars

    NASA Technical Reports Server (NTRS)

    Friend, David B.

    1990-01-01

    This paper presents measurements of terminal velocities of OB stars which are rapid rotators, based on archival high-dispersion IUE spectra of the C IV resonance doublet. The terminal velocities of the most rapidly rotating stars appear to be systematically lower than those of the less rapidly rotating stars (at least for the cooler stars), although the number of very rapid rotators is only three. The modified line-radiation driven wind model of Friend and Abbott, which takes into account the finite size of the star as well as its rotation, predicts that the terminal velocity should drop with increasing rotational velocity. However, when a smaller but very homogeneous subset of the data is used (BO giants only), the correlation between terminal velocity and rotational velocity disappears.

  15. The Revised OB-1 Method for Metal-Water Systems

    SciTech Connect

    Westfall, Robert Michael; Wright, Richard Q

    2010-01-01

    The OB-1 method for the calculation of the minimum critical mass (mcm) of fissile actinides in metal/water systems was described in a 2008 Nuclear Science and Engineering (NS&E) article. The purpose of the present work is to update and expand the application of this method with current nuclear data, including data uncertainties. The mcm and the hypothetical fissile metal density ({rho}{sub F}) in grams of metal/liter are obtained by a fit to values predicted with transport calculations. The input parameters required are thermal values for fission and absorption cross sections and nubar. A factor of ({radical}{pi})/2 is used to convert to Maxwellian averaged values. The uncertainties for the fission and capture cross sections and the estimated nubar uncertainties are used to determine the uncertainties in the mcm, either in percent or grams.

  16. The OB association LH 58 in the Large Magellanic Cloud

    NASA Technical Reports Server (NTRS)

    Garmany, Catharine D.; Massey, Philip; Parker, Joel WM.

    1994-01-01

    We present charge coupled device (CCD) photometry and spectroscopy for stars in Lucke-Hodge 58, an isolated OB association in the Large Magellanic Cloud (LMC) northwest of 30 Doradus. The photometric catalog contains 839 stars with UBV magnitudes complete to V approximately = 19. We have obtained spectra and classified 35 stars; combined with previous published spectral types, we find 22 O-type stars. The earliest type is O3-4 V, and there are three Wolf-Rayet (WR) stars in the association. The slope of the initial mass function, Gamma = -1.7 +/- 0.3, is in good agreement with other LMC associations. The presence of several evolved supergiants with masses about 15-25 solar mass suggests that some star formation took place as early as 10 million years ago, but the majority of stars formed coevally within the past few million years.

  17. OB-fold domains: a snapshot of the evolution of sequence, structure and function.

    PubMed

    Arcus, Vickery

    2002-12-01

    The OB-fold is found in all three kingdoms and is well represented in both sequence and structural databases. The OB-fold is a five-stranded closed beta barrel and the majority of OB-fold proteins use the same face for ligand binding or as an active site. Different OB-fold proteins use this 'fold-related binding face' to, variously, bind oligosaccharides, oligonucleotides, proteins, metal ions and catalytic substrates. Recently, a number of new structures with OB-folds have been reported that augment the variation seen for this set of proteins whilst conserving the characteristic fold and binding face. The conservation of fold and a functional binding face amongst many structures provides a model for investigating the evolutionary trajectory of sequence, structure and function. PMID:12504685

  18. The Puppis region and the last crusade for faint OB stars

    NASA Astrophysics Data System (ADS)

    Orsatti, Ana M.

    1992-08-01

    UBV photoelectric and photographic measurements of OB stars from a list of 397 OB stars and 5 early-type supergiants and from the Luminous Stars Survey are presented. The galactic distribution of the OB stars in the region shows concentrations around the open clusters Ruprecht 44 and Ruprecht 55, and the presence of an important grouping of young stars located far below the plane. The distribution in latitude shows that young stars in the region are not restricted to a thin sheet around the plane but are spread over negative latitudes reaching at least b = -5 deg. In longitude, the OB distribution exhibits a concentration of Ob stars in the interval 244-251 deg; this is argued to be due to the presence of the local arm extension.

  19. The Orion OB1 association. 1: Stellar content

    NASA Technical Reports Server (NTRS)

    Brown, A. G. A.; De Geus, E. J.; De Zeeuw, P. T.

    1994-01-01

    Walfraven photometry of established and probable members of the Orion OB1 association is presented. Effective temperature, surface gravity, luminosity and mass are derived for all stars, using atmosphere model by Kurucz (1979). Absolute magnitudes are calculated using the Straizys and Kuriliene (1981) tables. Distance moduli and visual extinctions are determined. A comparison of the visual extinctions to IRAS 100 micrometers data shows that the near edge of the Orion A and B clouds lies at a distance of approximately 320 pc, while the far edge is at approximately 500 pc. A method for deriving the ages of the subgroups by comparing theoretical isochrones to the observations in the log g, log T(sub eff) plane is presented. The derived ages suggest, contrary to earlier studies, that subgroup 1b is younger than 1c, which can possibly be explained by past geometries of the system of stars and gas. The initial mass function for Orion OB1 is derived with the aid of the Kolmogorov-Smirnoff test. Through extensive simulations, we show that it is very difficult to derive accurately the Initial Mass Function (IMF) from the available data. To within somewhat weak limits the IMF is found to be of the form xi(log M) = AM(exp -1.7 +/- 0.2) for all subgroups. The energy output of the subgroups in the form of stellar winds and supernovae is calculated and compared to the observed size and expansion velocity of the Orion-Eridanus bubble. It is shown that the energy output of the association can account for the morphology and kinematics of the interstellar medium (ISM).

  20. Dusty OB Stars in the Small Magellanic Cloud. I. Optical Spectroscopy Reveals Predominantly Main-sequence OB Stars

    NASA Astrophysics Data System (ADS)

    Sheets, Holly A.; Bolatto, Alberto D.; van Loon, Jacco Th.; Sandstrom, Karin; Simon, Joshua D.; Oliveira, Joana M.; Barbá, Rodolfo H.

    2013-07-01

    We present the results of optical spectroscopic follow-up of 125 candidate main sequence OB stars in the Small Magellanic Cloud (SMC) that were originally identified in the S3MC infrared imaging survey as showing an excess of emission at 24 μm indicative of warm dust, such as that associated with a transitional or debris disks. We use these long-slit spectra to investigate the origin of the 24 μm emission and the nature of these stars. A possible explanation for the observed 24 μm excess, that these are emission line stars with dusty excretion disks, is disproven for the majority of our sources. We find that 88 of these objects are normal stars without line emission, with spectral types mostly ranging from late-O to early-B; luminosity classes from the literature for a sub-set of our sample indicate that most are main-sequence stars. We further identify 17 emission-line stars, 7 possible emission-line stars, and 5 other objects with forbidden-line emission in our sample. We discover a new O6 Iaf star; it exhibits strong He II 4686 Å emission but relatively weak N III 4640 Å emission which we attribute to the lower nitrogen abundance in the SMC. Two other objects are identified with planetary nebulae, one with a young stellar object, and two with X-ray binaries. To shed additional light on the nature of the observed 24 μm excess we use optical and infrared photometry to estimate the dust properties of the objects with normal O and B star spectra and compare these properties to those of a sample of hot spots in the Galactic interstellar medium (ISM). We find that the dust properties of the dusty OB star sample resemble the properties of the Galactic sample of hot spots. Some may be runaway systems with bow-shocks resulting from a large velocity difference between star and ISM. We further investigate the nature of these dusty OB stars in a companion paper presenting mid-infrared spectroscopy and additional imaging.

  1. DUSTY OB STARS IN THE SMALL MAGELLANIC CLOUD. I. OPTICAL SPECTROSCOPY REVEALS PREDOMINANTLY MAIN-SEQUENCE OB STARS

    SciTech Connect

    Sheets, Holly A.; Bolatto, Alberto D.; Van Loon, Jacco Th.; Oliveira, Joana M.; Sandstrom, Karin; Simon, Joshua D.; Barba, Rodolfo H.

    2013-07-10

    We present the results of optical spectroscopic follow-up of 125 candidate main sequence OB stars in the Small Magellanic Cloud (SMC) that were originally identified in the S{sup 3}MC infrared imaging survey as showing an excess of emission at 24 {mu}m indicative of warm dust, such as that associated with a transitional or debris disks. We use these long-slit spectra to investigate the origin of the 24 {mu}m emission and the nature of these stars. A possible explanation for the observed 24 {mu}m excess, that these are emission line stars with dusty excretion disks, is disproven for the majority of our sources. We find that 88 of these objects are normal stars without line emission, with spectral types mostly ranging from late-O to early-B; luminosity classes from the literature for a sub-set of our sample indicate that most are main-sequence stars. We further identify 17 emission-line stars, 7 possible emission-line stars, and 5 other objects with forbidden-line emission in our sample. We discover a new O6 Iaf star; it exhibits strong He II 4686 A emission but relatively weak N III 4640 A emission which we attribute to the lower nitrogen abundance in the SMC. Two other objects are identified with planetary nebulae, one with a young stellar object, and two with X-ray binaries. To shed additional light on the nature of the observed 24 {mu}m excess we use optical and infrared photometry to estimate the dust properties of the objects with normal O and B star spectra and compare these properties to those of a sample of hot spots in the Galactic interstellar medium (ISM). We find that the dust properties of the dusty OB star sample resemble the properties of the Galactic sample of hot spots. Some may be runaway systems with bow-shocks resulting from a large velocity difference between star and ISM. We further investigate the nature of these dusty OB stars in a companion paper presenting mid-infrared spectroscopy and additional imaging.

  2. Loss of FXR Protects against Diet-Induced Obesity and Accelerates Liver Carcinogenesis in ob/ob Mice

    PubMed Central

    Ge, Xuemei; Heemstra, Lydia A.; Chen, Wei-Dong; Xu, Jiesi; Smith, Joseph L.; Ma, Huiyan; Kasim, Neda; Edwards, Peter A.; Novak, Colleen M.

    2012-01-01

    Farnesoid X receptor (FXR) is known to play important regulatory roles in bile acid, lipid, and carbohydrate metabolism. Aged (>12 months old) Fxr−/− mice also develop spontaneous liver carcinomas. In this report, we used three mouse models to investigate the role of FXR deficiency in obesity. As compared with low-density lipoprotein receptor (Ldlr) knockout (Ldlr−/−) mice, the Ldlr−/−Fxr−/− double-knockout mice were highly resistant to diet-induced obesity, which was associated with increased expression of genes involved in energy metabolism in the skeletal muscle and brown adipose tissue. Such a striking effect of FXR deficiency on obesity on an Ldlr−/− background led us to investigate whether FXR deficiency alone is sufficient to affect obesity. As compared with wild-type mice, Fxr−/− mice showed resistance to diet-induced weight gain. Interestingly, only female Fxr−/− mice showed significant resistance to diet-induced obesity, which was accompanied by increased energy expenditure in these mice. Finally, we determined the effect of FXR deficiency on obesity in a genetically obese and diabetic mouse model. We generated ob−/−Fxr−/− mice that were deficient in both Leptin and Fxr. On a chow diet, ob−/−Fxr−/− mice gained less body weight and had reduced body fat mass as compared with ob/ob mice. In addition, we observed liver carcinomas in 43% of young (<11 months old) Ob−/−Fxr−/− mice. Together these data indicate that loss of FXR prevents diet-induced or genetic obesity and accelerates liver carcinogenesis under diabetic conditions. PMID:22261820

  3. Irradiated interfaces in the Ara OB1, Carina, Eagle Nebula, and Cyg OB2 massive star formation regions

    NASA Astrophysics Data System (ADS)

    Hartigan, P.; Palmer, J.; Cleeves, L. I.

    2012-12-01

    Regions of massive star formation offer some of the best and most easily-observed examples of radiation hydrodynamics. Boundaries where fully-ionized H II regions transition to neutral/molecular photodissociation regions (PDRs) are of particular interest because marked temperature and density contrasts across the boundaries lead to evaporative flows and fluid dynamical instabilities that can evolve into spectacular pillar-like structures. When detached from their parent clouds, pillars become ionized globules that often harbor one or more young stars. H2 molecules at the interface between a PDR and an H II region absorb ultraviolet light from massive stars, and the resulting fluoresced infrared emission lines are an ideal way to trace this boundary independent of obscuring dust. This paper presents H2 images of four regions of massive star formation that illustrate different types of PDR boundaries. The Ara OB1 star formation region contains a striking long wall that has several wavy structures which are present in H2, but the emission is not particularly bright because the ambient UV fluxes are relatively low. In contrast, the Carina star formation region shows strong H2 fluorescence both along curved walls and at the edges of spectacular pillars that in some cases have become detached from their parent clouds. The less-spectacular but more well-known Eagle Nebula has two regions that have strong fluorescence in addition to its pillars. While somewhat older than the other regions, Cyg OB2 has the highest number of massive stars of the regions surveyed and contains many isolated, fluoresced globules that have head-tail morphologies which point towards the sources of ionizing radiation. These images provide a collection of potential astrophysical analogs that may relate to ablated interfaces observed in laser experiments of radiation hydrodynamics.

  4. miR-696 plays a role in hepatic gluconeogenesis in ob/ob mice by targeting PGC-1α.

    PubMed

    Fang, Zhijuan; Li, Peng; Jia, Wenhui; Jiang, Ting; Wang, Zhongyun; Xiang, Yang

    2016-09-01

    MicroRNAs (miRNAs or miRs) are known to play a vital role in type 2 diabetes, and peroxisome proliferator-activated receptor-γ (PPARγ) coactivator-1α (PGC-1α) is involved in the pathogenesis of hepatic insulin resistance. However, the correlation, if any, between PGC-1α and miRNAs in the disease has not yet been determined. Thus, in the present study, we aimed to examine the correlation between PGC-1α and miRNAs in diabetes. For this purpose, we used primary hepatocytes isolated from C57BL/6 mice and ob/ob mice. First, we found an inverse correlation between miR‑696 and PGC-1α protein levels in vivo. Second, in vitro evidence demonstrated that PGC-1α expression was significantly decreased by infection with pre-miR‑696-LV, whereas infection with anti-miR‑696-LV increased the PGC-1α protein levels. Third, a luciferase reporter assay confirmed that miR‑696 directly recognizes a specific location within the 3'-untranslated region of PGC-1α transcripts. Furthermore, the biological consequences of miR‑696 targeting PGC-1α were determined by measuring the expression levels of the characteristic hepatic gluconeogenic enzyme, PEPCK, which is regulated by PGC-1α in the liver via the coactivation of transcription factors. Taken together, our findings demonstrate that miR‑696 plays an important role in the development of hepatic gluconeogenesis and insulin resistance through the inhibition of PGC-1α translation in the liver. PMID:27432632

  5. Design, Synthesis, and Application of OB2C Combinatorial Peptide and Peptidomimetic Libraries

    PubMed Central

    Liu, Ruiwu; Shih, Tsung-Chieh; Deng, Xiaojun; Anwar, Lara; Ahadi, Sara; Kumaresan, Pappanaicken; Lam, Kit S.

    2015-01-01

    The “one-bead two-compound” (OB2C) combinatorial library is constructed on topologically segregated trifunctional bilayer beads such that each bead has a fixed cell-capturing ligand and a random library compound co-displayed on its surface and a chemical coding tag (bar code) inside the bead. An OB2C library containing thousands to millions of compounds can be synthesized and screened concurrently within a short period of time. When live cells are incubated with such OB2C libraries, every bead will be coated with a monolayer of cells. The cell membranes of the captured cells facing the bead surface are exposed to the library compounds tethered to each bead. A specific biochemical or cellular response can be detected with an appropriate reporter system. The OB2C method enables investigators to rapidly discover synthetic molecules that not only interact with cell-surface receptors but can also stimulate or inhibit downstream cell signaling. To demonstrate this powerful method, one OB2C peptide library and two OB2C peptidomimetic libraries were synthesized and screened against Molt-4 lymphoma cells to discover “death ligands.” Apoptosis of the bead-bound cells was detected with immunocytochemistry using horseradish peroxidase (HRP)-conjugated anti-cleaved caspase-3 antibody and 3,3′-diaminobenzidine as a substrate. Two novel synthetic “death ligands” against Molt-4 cells were discovered using this OB2C library approach. PMID:25616322

  6. Design, synthesis, and application of OB2C combinatorial peptide and peptidomimetic libraries.

    PubMed

    Liu, Ruiwu; Shih, Tsung-Chieh; Deng, Xiaojun; Anwar, Lara; Ahadi, Sara; Kumaresan, Pappanaicken; Lam, Kit S

    2015-01-01

    The "one-bead two-compound" (OB2C) combinatorial library is constructed on topologically segregated trifunctional bilayer beads such that each bead has a fixed cell-capturing ligand and a random library compound co-displayed on its surface and a chemical coding tag (bar code) inside the bead. An OB2C library containing thousands to millions of compounds can be synthesized and screened concurrently within a short period of time. When live cells are incubated with such OB2C libraries, every bead will be coated with a monolayer of cells. The cell membranes of the captured cells facing the bead surface are exposed to the library compounds tethered to each bead. A specific biochemical or cellular response can be detected with an appropriate reporter system. The OB2C method enables investigators to rapidly discover synthetic molecules that not only interact with cell-surface receptors but can also stimulate or inhibit downstream cell signaling. To demonstrate this powerful method, one OB2C peptide library and two OB2C peptidomimetic libraries were synthesized and screened against Molt-4 lymphoma cells to discover "death ligands." Apoptosis of the bead-bound cells was detected with immunocytochemistry using horseradish peroxidase (HRP)-conjugated anti-cleaved caspase-3 antibody and 3,3'-diaminobenzidine as a substrate. Two novel synthetic "death ligands" against Molt-4 cells were discovered using this OB2C library approach. PMID:25616322

  7. Possible detection of π^0^-decay γ-ray emission from CYG OB2 by EGRET.

    NASA Astrophysics Data System (ADS)

    Chen, W.; White, R. L.; Bertsch, D.

    1996-12-01

    We report possible detection of π^0^-decay radiation from Cyg OB2, a nearby (1.7kpc) massive OB star association. The EGRET flux (>100MeV) maps clearly indicate a point source whose error circle includes both Cyg OB2 and Cyg X-3. We show that Cyg X-3 is unlikely to be the counterpart for the EGRET source, because of the marginal spatial consistency and the lack of the 4.8-hour modulation seen in other high energy emissions from Cyg X-3. If confirmed, this will be the first detection by EGRET of massive stars.

  8. D Recording of a 19-CENTURY OB River Ship

    NASA Astrophysics Data System (ADS)

    Pushkarev, A. A.; Zaytceva, O. V.; Vavulin, M. V.; Skorobogatova, A. Y.

    2016-06-01

    A 3D recording of a 19-century wooden ship discovered on the bank of the river Ob (Western Siberia) was performed in autumn 2015. The archaeologized ship was partly under water, partly lying ashore, buried under fluvial deposits. The 3D recording was performed in October, when the water level was at its lowest after clearing the area around the ship. A 3D recording at the place of discovery was required as part of the ship museumification and reconstruction project. The works performed were primarily aimed at preserving as much information about the object as possible. Given the location and peculiar features of the object, a combination of close-range photogrammetry and aerial photography was considered to be the best possible solution for creating a high-quality 3D model. The dismantled ship was delivered to Nizhnevartovsk Museum of Local History in October 2015. The ship is going to be reassembled using the created 3D model to be exhibited in the museum. The resulting models are also going to be used to make a virtual 3D reconstruction of the ship in the future. We shot a stereoscopic video for Nizhnevartovsk Museum of Local History to let visitors see the place of discovery and explore the ship in greater details. Besides, 3D printing allowed for creating a miniature of the ship, which is also going to be included in the exposition devoted to this unique discovery.

  9. Insulin releasing effects of adrenocorticotropin (ACTH 1-39) and ACTH fragments (1-24 and 18-39) in lean and genetically obese hyperglycaemic (ob/ob) mice.

    PubMed

    Bailey, C J; Flatt, P R

    1987-01-01

    An excessive insulin releasing effect of adrenocorticotropic hormone (ACTH) and ACTH fragments has been considered as a possible factor contributing to the hyperinsulinaemia of genetically obese hyperglycaemic (ob/ob) mice. To investigate this possibility, plasma glucose and insulin responses of 11- to 14-week-old fed lean and ob/ob mice were examined after intraperitoneal administration of ACTH 1-39, ACTH 1-24 and ACTH 18-39, each at a dose of 25 nmol/mouse (50-115 micrograms/mouse). ACTH 1-39 produced a marked and rapid increase of plasma insulin in both lean and ob/ob mice, the effect being much greater in the ob/ob mutant (maximum increases of 5.5 +/- 1.5 and 46.1 +/- 4.1 ng/ml at 10 min in lean and ob/ob mice respectively, P less than 0.001). In lean mice plasma glucose concentrations showed a protracted decreased (maximum decrease of 3.7 +/- 0.5 mmol/l at 120 min), whereas glucose concentrations were increased (maximum increase of 4.2 +/- 1.3 mmol/l at 60 min) in ob/ob mice. ACTH 1-24 produced qualitatively similar but generally smaller effects than ACTH 1-39, while ACTH 18-39 did not significantly affect glucose and insulin concentrations. In 24 h fasted mice, ACTH 1-39 produced similar but generally smaller effects than in fed mice. The results suggest that the effects of ACTH on glucose and insulin homoeostasis are conferred by the N-terminal 1-24 sequence, and ACTH may exert acute effects which contribute to the hyperinsulinaemia and hyperglycaemia of ob/ob mice. PMID:3038765

  10. Study of eclipsing binary and multiple systems in ob associations. II. The cygnus ob region: V443 Cyg, V456 Cyg, and V2107 Cyg

    SciTech Connect

    Bakış, V.; Bakış, H.; Hensberge, H.; Bilir, S.; Yılmaz, F.; Kıran, E.; Demircan, O.; Zejda, M.; Mikulášek, Z.

    2014-06-01

    Three presumably young eclipsing binary systems in the direction of the Cygnus OB1, OB3, and OB9 associations are studied. Component spectra are reconstructed and their orbits are determined using light curves and spectra disentangling techniques. V443 Cyg and V456 Cyg have circular orbits while the light curve of V2107 Cyg imposes a slightly eccentric orbit (e = 0.045 ± 0.03). V443 Cyg harbors F-type stars, not young early-A stars as previously suggested in the literature based solely on photometry. It appears to be situated in the foreground (distance 0.6 ± 0.2 kpc) of the young stellar populations in Cygnus. V456 Cyg, at a distance of 0.50 ± 0.03 kpc, consists of a slightly metal-weak A-type star and an early-F star. The age of both systems, on or very near to the main sequence, remains uncertain by an order of magnitude. V2107 Cyg is a more massive system (8.9 ± 2 and 4.5 ± 1.2 M {sub ☉}) at 1.5 ± 0.5 kpc and, also kinematically, a strong candidate-member of Cyg OB1. The more massive component is slightly evolved and appears to undergo non-radial βCep-type pulsations. The Doppler signal of the secondary is barely detectable. A more extensive, asteroseismological study is necessary to fix masses more precisely. Nevertheless, the position of the primary in the H-R diagram confines the age reasonably well to 20 ± 5 Myr, indicating that for Cyg OB1 has a similar extent of star formation history as that established for Cyg OB2.

  11. Study of Eclipsing Binary and Multiple Systems in OB Associations. II. The Cygnus OB Region: V443 Cyg, V456 Cyg, and V2107 Cyg

    NASA Astrophysics Data System (ADS)

    Bakış, V.; Hensberge, H.; Bilir, S.; Bakış, H.; Yılmaz, F.; Kıran, E.; Demircan, O.; Zejda, M.; Mikulášek, Z.

    2014-06-01

    Three presumably young eclipsing binary systems in the direction of the Cygnus OB1, OB3, and OB9 associations are studied. Component spectra are reconstructed and their orbits are determined using light curves and spectra disentangling techniques. V443 Cyg and V456 Cyg have circular orbits while the light curve of V2107 Cyg imposes a slightly eccentric orbit (e = 0.045 ± 0.03). V443 Cyg harbors F-type stars, not young early-A stars as previously suggested in the literature based solely on photometry. It appears to be situated in the foreground (distance 0.6 ± 0.2 kpc) of the young stellar populations in Cygnus. V456 Cyg, at a distance of 0.50 ± 0.03 kpc, consists of a slightly metal-weak A-type star and an early-F star. The age of both systems, on or very near to the main sequence, remains uncertain by an order of magnitude. V2107 Cyg is a more massive system (8.9 ± 2 and 4.5 ± 1.2 M ⊙) at 1.5 ± 0.5 kpc and, also kinematically, a strong candidate-member of Cyg OB1. The more massive component is slightly evolved and appears to undergo non-radial βCep-type pulsations. The Doppler signal of the secondary is barely detectable. A more extensive, asteroseismological study is necessary to fix masses more precisely. Nevertheless, the position of the primary in the H-R diagram confines the age reasonably well to 20 ± 5 Myr, indicating that for Cyg OB1 has a similar extent of star formation history as that established for Cyg OB2.

  12. Yhhu981, a novel compound, stimulates fatty acid oxidation via the activation of AMPK and ameliorates lipid metabolism disorder in ob/ob mice

    PubMed Central

    Zeng, Hong-liang; Huang, Su-ling; Xie, Fu-chun; Zeng, Li-min; Hu, You-hong; Leng, Ying

    2015-01-01

    Aim: Defects in fatty acid metabolism contribute to the pathogenesis of insulin resistance and obesity. In this study, we investigated the effects of a novel compound yhhu981 on fatty acid metabolism in vitro and in vivo. Methods: The capacity to stimulate fatty acid oxidation was assessed in C2C12 myotubes. The fatty acid synthesis was studied in HepG2 cells using isotope tracing. The phosphorylation of AMPK and acetyl-CoA carboxylase (ACC) was examined with Western blot analysis. For in vivo experiments, ob/ob mice were orally treated with yhhu981 acutely (300 mg/kg) or chronically (150 or 300 mg·kg−1·d−1 for 22 d). On the last day of treatment, serum and tissue samples were collected for analysis. Results: Yhhu981 (12.5–25 μmol/L) significantly increased fatty acid oxidation and the expression of related genes (Sirt1, Pgc1α and Mcad) in C2C12 myotubes, and inhibited fatty acid synthesis in HepG2 cells. Furthermore, yhhu981 dose-dependently increased the phosphorylation of AMPK and ACC in both C2C12 myotubes and HepG2 cells. Compound C, an AMPK inhibitor, blocked fatty acid oxidation in yhhu981-treated C2C12 myotubes and fatty acid synthesis decrease in yhhu981-treated HepG2 cells. Acute administration of yhhu981 decreased the respiratory exchange ratio in ob/ob mice, whereas chronic treatment with yhhu981 ameliorated the lipid abnormalities and ectopic lipid deposition in skeletal muscle and liver of ob/ob mice. Conclusion: Yhhu981 is a potent compound that stimulates fatty acid oxidation, and exerts pleiotropic effects on lipid metabolism by activating AMPK. PMID:25732571

  13. Spectroscopic Confirmation of the Dragonfish Association: The Galaxy's Most Luminous OB Association

    NASA Astrophysics Data System (ADS)

    Rahman, Mubdi; Moon, Dae-Sik; Matzner, Christopher D.

    2011-12-01

    Young OB associations with masses greater than 104 M ⊙ have been inferred to exist in the Galaxy but have largely evaded detection. Recently, a candidate OB association has been identified within the most luminous star-forming complex in the Galaxy, the Dragonfish Nebula. We identify 18 young, massive stars with near-infrared spectroscopy from a sample of 50 members within the candidate OB association, including 15 O-type and 3 luminous blue variables or Wolf-Rayet stars. This number matches the expected yield of massive stars from the candidate association, confirming its existence and ability to power the parent star-forming complex. These results demonstrate the existence of a 105 M ⊙ OB association, more powerful than any previously known in the Galaxy, comparable in mass only to Westerlund 1. Further, the results also validate the color selection method used to identify the association, adding credence to others discovered in the same way.

  14. Optical Spectroscopic Confirmation of OB Star Candidates in HII Regions (2)

    NASA Astrophysics Data System (ADS)

    Tsujimoto, Masahiro; Kobayashi, Naoto; Wang, Junfeng

    2008-02-01

    The number of OB stars (earlier than B2) is one of the most basic quantities to characterize massive star clusters. We studied several massive clusters using the Chandra X-ray Observatory and found 40--60 new OB star candidates in NGC6357, NGC6231, and NGC6334. The numbers are much larger than those previously known and their spatial distribution extends beyond the central OB associations. We propose a multi-object spectroscopy of these sources to confirm their nature. Using hydrogen and helium emission/absorption lines in the 4000-5000 A spectra and comparing with the OB spectral atlas in the literature, we can determine spectral sub-types and luminosity classes of these sources.

  15. OB-RL silencing inhibits the thermoregulatory ability of Great Roundleaf Bats (Hipposideros armiger).

    PubMed

    Zhu, Tengteng; Yuan, Lihong; Jones, Gareth; Hua, Panyu; He, Guimei; Chen, Jinping; Zhang, Shuyi

    2014-08-01

    Previous studies have shown that the hormone Leptin has an important role in mammalian heterothermy by regulating metabolism and food intake via lipolysis, as well as adaptive evolution of Leptin in heterothermic bats driven by selected pressure. However, the mechanism of Leptin in heterothermic regulation in mammals is unknown. By combining previous results, we speculated that the Leptin signaling pathway mediated by OB-RL (Leptin receptor long form) in the hypothalamus is important. OB-RL is one of the products of db gene and mainly distributed in the hypothalamus. In this study, we used OB-RL as a molecular marker, combining with the RNA interference technology and physiological/molecular analyses with Hipposideros armiger (a hibernating bat species) as an animal model, to explore the mechanism of Leptin in heterothermic regulation. Our data showed that all of four anti-OB-RL shRNA lentivirus significantly inhibited OB-RL expression (>90%), and the interference efficiency of PSC1742 lentivirus reached the highest value. In situ hybridization proved that PSC1742 lentivirus significantly decreased the OB-RL expression in the hypothalamus, especially in the ventromedial hypothalamic nucleus (VHM, 86.6%). Physiological analysis demonstrated that the thermoregulatory ability of bats (e.g., reducing core body temperature and heart rate) was significantly depressed after OB-RL silencing in the hypothalamus, and animals could not enter torpor state. Our study for the first time proved that the knock-down of OB-RL expression in hypothalamus inhibits heterothermic regulation of bats, and also provided the clues for further analyzing the mechanism of Leptin in the heterothermic regulation of mammals. PMID:24815886

  16. Intrinsic ultraviolet (912-3200 A) energy distribution of OB stars

    NASA Technical Reports Server (NTRS)

    Longo, Renata; Stalio, Roberto; Polidan, Ronald S.; Rossi, Lucio

    1989-01-01

    Low-dispersion 500-3200-A spectra of 20 OB stars are used to derive three individual extinction curves in the wavelength range 912-1200 A. The dependence of the intrinsic UV fluxes of OB stars on both spectral type and luminosity is demonstrated. UV color indices are proposed. The results confirm the previous finding that O star spectra are not significantly bluer than the spectrum of the B0 main-sequence star Upsilon Ori.

  17. ObsPack: a framework for the preparation, delivery, and attribution of atmospheric greenhouse gas measurements

    NASA Astrophysics Data System (ADS)

    Masarie, K. A.; Peters, W.; Jacobson, A. R.; Tans, P. P.

    2014-12-01

    Observation Package (ObsPack) is a framework designed to bring together atmospheric greenhouse gas observations from a variety of sampling platforms, prepare them with specific applications in mind, and package and distribute them in a self-consistent and well-documented product. Data products created using the ObsPack framework (called "ObsPack products") are intended to support carbon cycle modeling studies and represent a next generation of value-added greenhouse gas observation products modeled after the cooperative GLOBALVIEW products introduced in 1996. Depending on intended use, ObsPack products may include data in their original form reformatted using the ObsPack framework or may contain derived data consisting of averages, subsets, or smoothed representations of original data. All products include extensive ancillary information (metadata) intended to help ensure the data are used appropriately, their calibration and quality assurance history are clearly described, and that individuals responsible for the measurements (data providers or principal investigators (PIs)) are properly acknowledged for their work. ObsPack products are made freely available using a distribution strategy designed to improve communication between data providers and product users. The strategy includes a data usage policy that requires users to directly communicate with data providers and an automated e-mail notification system triggered when a product is accessed. ObsPack products will be assigned a unique digital object identifier (DOI) to ensure each product can be unambiguously identified in scientific literature. Here we describe the ObsPack framework and its potential role in supporting the evolving needs of both data providers and product users.

  18. ObsPack: a framework for the preparation, delivery, and attribution of atmospheric greenhouse gas data

    NASA Astrophysics Data System (ADS)

    Masarie, K. A.; Peters, W.; Jacobson, A. R.; Tans, P. P.

    2014-09-01

    Observation Package (ObsPack) is a framework designed to bring together atmospheric greenhouse gas observations from a variety of sampling platforms, prepare them with specific applications in mind, and package and distribute them in a self-consistent and well-documented product. Data products created using the ObsPack framework (called "ObsPack products") are intended to support carbon cycle modeling studies and represent the next generation of value-added greenhouse gas observation products modeled after the cooperative GLOBALVIEW products introduced in 1996. Depending on intended use, ObsPack products may include data in their original form reformatted using the ObsPack framework or may contain derived data consisting of averages, subsets or smoothed representations of original data. All products include extensive ancillary information (metadata) intended to help ensure the data are used appropriately, their calibration and quality assurance history are clearly described, and that individuals responsible for the measurements (data providers or principal investigators (PIs)) are properly acknowledged for their work. ObsPack products are made freely available using a distribution strategy designed to improve communication between data providers and product users. The strategy includes a data usage policy that requires users to directly communicate with data providers and an automated e-mail notification system triggered when a product is accessed. ObsPack products will be assigned a unique Digital Object Identifier (DOI) to ensure each product can be unambiguously identified in scientific literature. Here we describe the ObsPack framework and its potential role in supporting the evolving needs of both data providers and product users.

  19. Developmental and hormonal regulation of leptin receptor (Ob-R) messenger ribonucleic acid expression in rat testis.

    PubMed

    Tena-Sempere, M; Pinilla, L; Zhang, F P; González, L C; Huhtaniemi, I; Casanueva, F F; Dieguez, C; Aguilar, E

    2001-02-01

    In target tissues, leptin receptor (Ob-R) gene expression results in an array of alternatively spliced isoforms (Ob-Ra to Ob-Rf) with different functional features. Recent evidence has pointed to a direct role of leptin in the control of testicular function. However, complete elucidation of the pattern of Ob-R gene expression in the male gonad is still pending. The focus of this study was to characterize in detail the developmental pattern of expression and hormonal regulation of Ob-R gene in rat testis. To this end, the overall expression of Ob-R mRNA was compared to that of the fully functional, long Ob-Rb isoform in different experimental settings, using semiquantitative reverse transcription-polymerase chain reaction. Expression of Ob-R mRNA was detected in testes from 15-, 30-, 45-, and 75-day-old rats at rather constant relative levels. In contrast, testicular expression of Ob-Rb mRNA was higher in pubertal testes (15- to 30-day-old rats) and declined in adulthood. In testes from 30-day-old animals, analysis of isoform distribution revealed that, in addition to abundant Ob-Rb mRNA levels, expression of Ob-Ra, Ob-Rf, and, to a lesser extent, Ob-Rc and Ob-Re messages is detected. Testicular Ob-R mRNA expression appeared sensitive to neonatal imprinting as neonatal treatment with estradiol benzoate (500 microg/rat; Day 1 postpartum) resulted in a persistent increase (P: < 0.01) in the relative expression level of Ob-R mRNA, a phenomenon only partially mimicked by neonatal suppression of serum gonadotropins by means of LHRH-antagonist administration. In addition, neonatal estrogenization differentially altered the pattern of expression of Ob-R isoforms in adult rat testis, as expression of Ob-Rb mRNA was decreased to undetectable levels, whereas that of Ob-Rc remained unaltered, and Ob-Ra, Ob-Rf, and, to a lesser extent, Ob-Re mRNA levels were significantly increased (P: < 0.01) by neonatal exposure to estrogen. Finally, down-regulation of testicular Ob-R gene

  20. Silencing of OB-RGRP in mouse hypothalamic arcuate nucleus increases leptin receptor signaling and prevents diet-induced obesity

    PubMed Central

    Couturier, Cyril; Sarkis, Chamsy; Séron, Karin; Belouzard, Sandrine; Chen, Patty; Lenain, Aude; Corset, Laetitia; Dam, Julie; Vauthier, Virginie; Dubart, Anne; Mallet, Jacques; Froguel, Philippe; Rouillé, Yves; Jockers, Ralf

    2007-01-01

    Obesity is a major public health problem and is often associated with type 2 diabetes mellitus, cardiovascular disease, and metabolic syndrome. Leptin is the crucial adipostatic hormone that controls food intake and body weight through the activation of specific leptin receptors (OB-R) in the hypothalamic arcuate nucleus (ARC). However, in most obese patients, high circulating levels of leptin fail to bring about weight loss. The prevention of this “leptin resistance” is a major goal for obesity research. We report here a successful prevention of diet-induced obesity (DIO) by silencing a negative regulator of OB-R function, the OB-R gene-related protein (OB-RGRP), whose transcript is genetically linked to the OB-R transcript. We provide in vitro evidence that OB-RGRP controls OB-R function by negatively regulating its cell surface expression. In the DIO mouse model, obesity was prevented by silencing OB-RGRP through stereotactic injection of a lentiviral vector encoding a shRNA directed against OB-RGRP in the ARC. This work demonstrates that OB-RGRP is a potential target for obesity treatment. Indeed, regulators of the receptor could be more appropriate targets than the receptor itself. This finding could serve as the basis for an approach to identifying potential new therapeutic targets for a variety of diseases, including obesity. PMID:18042720

  1. Therapeutic effects of the dipeptidyl peptidase-IV inhibitor, sitagliptin, on non-alcoholic steatohepatitis in FLS-ob/ob male mice.

    PubMed

    Onoyama, Takumi; Koda, Masahiko; Okamoto, Toshiaki; Kishina, Manabu; Matono, Tomomitsu; Sugihara, Takaaki; Murawaki, Yoshikazu

    2015-11-01

    Non-alcoholic steatohepatitis is characterized by hepatic fat accumulation, inflammation and varying degrees of fibrosis. The dipeptidyl peptidase‑IV enzyme is important in glucose metabolism, as well as lipid accumulation, extracellular matrix metabolism and immune stimulation. Furthermore, the enzyme activity of dipeptidyl peptidase‑IV is known to be increased in non‑alcoholic steatohepatitis. Therefore, dipeptidyl peptidase‑IV inhibitors are potential therapeutic agents for non‑alcoholic steatohepatitis. The present study assessed the therapeutic effects of sitagliptin, a dipeptidyl peptidase‑IV inhibitor, on non‑alcoholic steatohepatitis using fatty liver Shionogi‑ob/ob male mice. Sitagliptin (2 mg/kg/day; n=10) or placebo (control; n=10) was orally administered to fatty liver Shionogi‑ob/ob mice for 12 weeks, and hepatic steatosis, fibrosis, inflammation and oxidative stress were assessed in comparison with the controls. Sitagliptin administration reduced body weight and blood glucose levels, and improved hepatic fibrosis. It also inhibited the gene expression levels of fatty acid synthase, transforming growth factor‑β1, tissue inhibitor of metalloproteinases‑1, procollagen‑type 1, tumor necrosis factor‑α, monocyte chemoattractant protein‑1 and enhanced peroxisome proliferator activated receptor‑α. Furthermore, a marked attenuation of hepatic stellate cell activation and Kupffer cells was observed in the sitagliptin group. A decrease in oxidative stress and apoptosis was also observed. Sitagliptin attenuated the progression of hepatic fibrosis by improving lipid metabolism, inflammation and oxidative stress in non-alcoholic steatohepatitis. PMID:26397061

  2. Pycnogenol supplementation promotes lipolysis via activation of cAMP-dependent PKA in ob/ob mice and primary-cultured adipocytes.

    PubMed

    Ho, Jin-Nyoung; Kim, Ok-Kyung; Nam, Da-Eun; Jun, Woojin; Lee, Jeongmin

    2014-01-01

    This study investigated the PKA-dependent inhibitory effect of pycnogenol (Pyc) on lipolysis using ob/ob mice and primary mouse adipocytes. Supplementation of Pyc at 30 mg/kg significantly reduced body weight gain and visceral fat mass. The serum and hepatic triglyceride (TG) and total cholesterol (TC) levels were reduced by Pyc supplementation, and high density lipoprotein (HDL)-cholesterol level significantly increased. In addition, hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) mRNA levels increased with Pyc supplementation in adipose tissue of ob/ob mice. The treatment of primary cultured adipocytes with Pyc at 100 μg/mL significantly increased glycerol release, cAMP level by reduction of phosphodiestersae-3B (PDE3B), and HSL levels, but decreased protein levels of perilipin A and fatty acid synthetase (FAS). The PKA inhibitor (H89) clearly blocked the cellular levels of perilipin A and HSL, suggesting that Pyc promotes lipolysis of adipocytes through activation of cAMP-dependent PKA, resulting in induction of HSL and reduction of perilipin A. Therefore, this study may elucidate the possible mechanism of Pyc, which is a candidate for weight loss through stimulation of lipolysis. PMID:25866307

  3. Detached binary system MQ Centauri and its relation with Crux OB1 Association

    NASA Astrophysics Data System (ADS)

    Tunc, Efecan; Bakış, Volkan

    2016-07-01

    In this study, the astrophysical characteristics of eclipsing binary system MQ Cen and its relation with Crux OB1 association has been investigated by means of analysing high resolution (R ˜48000) spectra and light curves in Strömgren bands. Analysis of the available data yielded high precision parameters of the system which allowed us to derive reliable age and distance. The Fourier disentangling method was applied to the composite spectra of MQ Cen for reconstructing the component spectra. The modeling of the high resolution spectra revealed that the secondary component of MQ Cen rotates slower than the synchronous rotation velocity unlike the primary star which is in synchronous rotation. The photometric distance of the binary was found to be consistent with the distance given for Crux OB1 associaton (2.7 kpc) by Kaltcheva and Georgiev (1993). Space velocity of the system also supports the membership of MQ Cen to the Crux OB1 association. Age estimation was made by comparing the positions of components on HR diagram with isochrones. Conclusion shows that MQ Cen is older than the age determined for Crux OB1 association in the literature. This result implies that MQ Cen may belong to one of the older subgroups in the Crux OB1 association complex and the stellar formation in the complex started long ago than previously predicted. The evolutionary status of the component stars suggest that both components have reached the final stage of their Main Sequence lifetime and proceed to the Giant Branch. KEYWORDS: Absolute parameters, Cru OB1, eclipsing binaries, MQ Cen, OB associations, spectral disentangling.

  4. Towards a better way of quantifying uncertainty in the E-OBS temperature dataset

    NASA Astrophysics Data System (ADS)

    Cornes, Richard; van den Besselaar, Else; Jones, Phil; van der Schrier, Gerard; Verver, Ge

    2016-04-01

    E-OBS is a widely used gridded dataset that provides daily resolution data from 1950 to "present-day" across Europe and is constructed by interpolating station data contained in the ECA&D database. While E-OBS provides several gridded variables, the focus in this study is uncertainty in the gridded temperature data. A measure of error is currently included in the dataset that aims to provide an estimate of the uncertainty of the data at each grid-box for each day. This uncertainty estimate is mainly a function of the density of the input station data and is particularly important as the quantity of these data vary spatially and temporally; this has a large effect on the reliability of the gridded data. However, the uncertainty measure currently provided in E-OBS remains one of the least-used components of the dataset by end-users. This uncertainty calculation is based on a combination of monthly error values, using Bayesian standard error estimates derived from monthly climatology values, and daily error values derived from interpolation variance. In this analysis we present an alternative way of quantifying uncertainty in the E-OBS data for the three temperature variables (maximum, minimum and mean daily temperature) using a multiple realization (ensemble) approach. The established E-OBS gridding method is retained but an ensemble of grids is produced using conditional stochastic simulation. This ensemble approach would seem to be the best way of incorporating uncertainty measures into the existing E-OBS gridding scheme, whilst providing a measure of uncertainty that should be of use to many users of the E-OBS data.

  5. Non-Thermal Emission from the massive stellar association Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Fenech, Danielle Marie; Prinja, Raman; Morford, Jack

    2015-08-01

    The Cygnus OB2 association is located in the Galactic Cygnus X region at a distance of 1.4 kpc, making it one of the closest young massive stellar clusters. Cyg OB2 is not only very rich in stellar density but also in its diversity. It is known to contain a rich population of massive stars including almost 2600 OB stars, a large number of binaries (including a collection of some of the most interesting radio emitting colliding-wind binaries), and a considerable number of pre-main sequence stars.We report here on the first results from The Cyg OB2 Radio Survey (COBRaS), which is a UCL-led e-MERLIN legacy project to provide a deep-field radio mapping of the Cygnus OB2 association. The project has been awarded a total allocation of 252 hours at C-band (5GHz) and 42 hours at L-band (1.6GHz) to image the core of the cluster.We discuss in particular the presence of non-thermal radio emission at 20 cm (L-band), and its potential as a highly efficient way to identify binaries via single-epoch observations, particularly for colliding-wind binaries. COBRaS data will provide a powerful tool for establishing binary incidence in Cyg~OB2, specifically in the difficult intermediate-period range (1--100~yr). Knowing the binary frequency over the whole period range is important for population synthesis.Additionally, Weak-lined T Tauri (WTT) stars in Cyg OB2 also emit non-thermal radiation from magnetically active regions. Hence these observations will be used to detect the considerable population of younger stars.Ultimately, we aim to assemble a substantial and uniquely sensitive radio dataset, which will be exploited to address several fundamentally important areas of stellar astrophysics, including mass-loss, binary frequency, stellar cluster dynamics, and triggered star-formation.

  6. Parameterization of DFTB3/3OB for Sulfur and Phosphorus for Chemical and Biological Applications

    PubMed Central

    2015-01-01

    We report the parametrization of the approximate density functional tight binding method, DFTB3, for sulfur and phosphorus. The parametrization is done in a framework consistent with our previous 3OB set established for O, N, C, and H, thus the resulting parameters can be used to describe a broad set of organic and biologically relevant molecules. The 3d orbitals are included in the parametrization, and the electronic parameters are chosen to minimize errors in the atomization energies. The parameters are tested using a fairly diverse set of molecules of biological relevance, focusing on the geometries, reaction energies, proton affinities, and hydrogen bonding interactions of these molecules; vibrational frequencies are also examined, although less systematically. The results of DFTB3/3OB are compared to those from DFT (B3LYP and PBE), ab initio (MP2, G3B3), and several popular semiempirical methods (PM6 and PDDG), as well as predictions of DFTB3 with the older parametrization (the MIO set). In general, DFTB3/3OB is a major improvement over the previous parametrization (DFTB3/MIO), and for the majority cases tested here, it also outperforms PM6 and PDDG, especially for structural properties, vibrational frequencies, hydrogen bonding interactions, and proton affinities. For reaction energies, DFTB3/3OB exhibits major improvement over DFTB3/MIO, due mainly to significant reduction of errors in atomization energies; compared to PM6 and PDDG, DFTB3/3OB also generally performs better, although the magnitude of improvement is more modest. Compared to high-level calculations, DFTB3/3OB is most successful at predicting geometries; larger errors are found in the energies, although the results can be greatly improved by computing single point energies at a high level with DFTB3 geometries. There are several remaining issues with the DFTB3/3OB approach, most notably its difficulty in describing phosphate hydrolysis reactions involving a change in the coordination number of

  7. Search for OB stars running away from young star clusters. I. NGC 6611

    NASA Astrophysics Data System (ADS)

    Gvaramadze, V. V.; Bomans, D. J.

    2008-11-01

    N-body simulations have shown that the dynamical decay of the young (~1 Myr) Orion Nebula cluster could be responsible for the loss of at least half of its initial content of OB stars. This result suggests that other young stellar systems could also lose a significant fraction of their massive stars at the very beginning of their evolution. To confirm this expectation, we used the Mid-Infrared Galactic Plane Survey (completed by the Midcourse Space Experiment satellite) to search for bow shocks around a number of young (⪉several Myr) clusters and OB associations. We discovered dozens of bow shocks generated by OB stars running away from these stellar systems, supporting the idea of significant dynamical loss of OB stars. In this paper, we report the discovery of three bow shocks produced by O-type stars ejected from the open cluster NGC 6611 (M16). One of the bow shocks is associated with the O9.5Iab star HD165319, which was suggested to be one of “the best examples for isolated Galactic high-mass star formation” (de Wit et al. 2005, A&A, 437, 247). Possible implications of our results for the origin of field OB stars are discussed.

  8. Leptin promotes proliferation and metastasis of human gallbladder cancer through OB-Rb leptin receptor.

    PubMed

    Zou, Hao; Liu, Yunxia; Wei, Dong; Wang, Tao; Wang, Kun; Huang, Songquan; Liu, Lixin; Li, Yuehua; Ge, Jiayun; Li, Xiao; Zhu, Hong; Wang, Lianmin; Zhao, Songling; Zhang, Xiaowen; Wang, Lin

    2016-07-01

    Emerging evidence has shown that leptin, an adipocyte-derived cytokine that is closely associated with obesity, play a significant role in carcinogenesis and tumorigenesis. However, its impact on gallbladder cancer (GBC) remains unclear. In this study, we firstly found that leptin and its functional receptor OB-Rb were significantly co-expressed in human GBC tissues and cell lines, the content of which were higher than those in normal human gallbladder tissues. Treatment with leptin promoted the proliferation, migration and invasion of GBC cells, which were attenuated by OB-Rb shRNA. Blocking in the G2/M period of cell cycle, increasing of MMP3 and MMP9, increasing of VEGF-C/D, activation of SOCS3/JAK2/p-STAT3 pathway was demonstrated after treatment with leptin. All of these positive responses were attenuated by OB-Rb receptor shRNA. Taken together, our findings suggest that leptin promoted the proliferation, migration and invasion of GBC cells by increasing OB-Rb expression through the SOCS3/JAK2/p-STAT3 signal pathway. Targeting the leptin/OB-Rb axis could be an attractive therapeutic strategy for treatment of GBC. PMID:27211817

  9. Efforts to Improve Immunization Coverage during Pregnancy among Ob-Gyns

    PubMed Central

    Jones, Katherine M.; Carroll, Sarah; Hawks, Debra; McElwain, Cora-Ann; Schulkin, Jay

    2016-01-01

    Background. Influenza and Tdap vaccines are vital factors for improving maternal and neonatal health outcomes. Methods. A prospective, longitudinal study was conducted to determine whether the American College of Obstetricians and Gynecologists' (ACOG's) efforts to increase ob-gyn use of their immunization toolkits and vaccination administration were successful. Pre- and postintervention questionnaires were mailed to a random sample of 1,500 ACOG members between August 2012 and July 2015. Results. Significantly more postintervention survey ob-gyns reported that they received the immunization toolkits than preintervention survey ob-gyns (84.5% versus 67.0%, p < .001). The large majority of ob-gyns from both surveys (76.9% versus 78.9%) reported that they offered or planned to offer influenza vaccinations to their patients for the 2012-2013 and 2014-2015 flu seasons. Postintervention survey respondents were significantly more likely than preintervention survey participants to report that they routinely offer Tdap vaccinations to all patients during pregnancy (76.8% versus 59.3%, p < .001). Conclusion. ACOG's efforts to improve ob-gyn use of immunization toolkits and vaccine administration appear to have been successful in several ways. ACOG's toolkits are an example of an effective intervention to overcome barriers to offering vaccines and help improve influenza and Tdap immunization coverage for pregnant women. PMID:26924918

  10. Gamma ray bursts versus OB associations: do they trigger star formation?

    NASA Astrophysics Data System (ADS)

    Efremov, Yu. N.; Ehlerová, S.; Palouš , J.

    1999-10-01

    We discuss differences in shapes, expansion velocities and fragmentation times of structures created by an energy deposition from a single Gamma-Ray Burst (GRB) and an OB association to the ISM. After the initial inflation, supershells produced by GRBs are almost static or slowly expanding, contrary to more rapidly expanding supershells created by OB associations. We discuss the position of the energy source relative to the symmetry plane of the galaxy: observed arc-like structures can be the most dense part of structures formed by an expansion from a source above or below the galactic plane. Arcs may also form if the expansion takes place inside a giant HI cloud. We try to reproduce the size, the age, and the average distance between OB associations in the Sextant region at the edge of LMC 4.

  11. An X-ray and Infrared Hunt for New Candidate Galactic OB Stars

    NASA Astrophysics Data System (ADS)

    Povich, Matthew S.; Alexander, Michael J.; Busk, Heather; Hanes, Richard J.; Feigelson, Eric; McSwain, M. Virginia; Townsley, Leisa K.

    2016-01-01

    Most young, massive OB stars produce X-ray emission through a variety of wind-driven shock processes, and individual massive stars are detectable out to several kpc distances in the Galactic plane using high-resolution imaging observations from the Chandra X-ray Observatory. We have developed a technique to identify known and new candidate OB stars by fitting model stellar atmospheres to the broadband infrared spectral energy distributions of X-ray-identified stars. Using this technique, we identified 94 candidate O- and early B-type stars in the Carina Nebula and an additional 98 candidates in 11 other Galactic Massive Star-Forming Regions. Visible-light and near-infrared follow-up spectroscopy of these candidates is ongoing, and initial results indicate that a majority of candidate massive stars will be spectroscopically confirmed as OB stars.

  12. Genome Sequence of the Obligate Methanotroph Methylosinus trichosporium Strain OB3b

    SciTech Connect

    Stein, Lisa Y.; Yoon, Sukhwan; Semrau, Jeremy D.; DiSpiritto, Alan A.; Crombie, Andrew; Murrell, J.; Vuilleumier, Stephane; Kalyuzhnaya, Marina G.; Den Camp, Huub J. M. Op; Bringel, Francoise O.; Bruce, David; Cheng, Jan-Fang; Copeland, A; Goodwin, Lynne A.; Han, Cliff; Hauser, Loren John; Jetten, MSM; Lajus, Aurelie; Lapidus, Alla L.; Lucas, Susan; Medigue, Claudine; Woyke, Tanja; Zeytun, Ahmet; Klotz, Martin G

    2010-01-01

    Methylosinus trichosporium OB3b (for "oddball" strain 3b) is an obligate aerobic methane-oxidizing alphaproteobacterium that was originally isolated in 1970 by Roger Whittenbury and colleagues. This strain has since been used extensively to elucidate the structure and function of several key enzymes of methane oxidation, including both particulate and soluble methane monooxygenase (sMMO) and the extracellular copper chelator methanobactin. In particular, the catalytic properties of soluble methane monooxygenase from M. trichosporium OB3b have been well characterized in context with biodegradation of recalcitrant hydrocarbons, such as trichloroethylene. The sequence of the M. trichosporium OB3b genome is the first reported from a member of the Methylocystaceae family in the order Rhizobiales.

  13. VizieR Online Data Catalog: OB association members in ACT+TRC Catalogs (Hoogerwerf, 2000)

    NASA Astrophysics Data System (ADS)

    Hoogerwerf, R.

    2000-05-01

    The Hipparcos Catalogue (Cat. I/239) contains members of nearby OB associations brighter than 12th magnitude in V. However, membership lists are complete only to magnitude V=7.3. In this paper we discuss whether proper motions listed in the `Astrographic Catalogue+Tycho' reference catalogue (ACT, Cat. I/246) and the Tycho Reference Catalogue (TRC, Cat. I/250), which are complete to V~10.5mag, can be used to find additional association members. Proper motions in the ACT/TRC have an average accuracy of ~3mas/yr. We search for ACT/TRC stars which have proper motions consistent with the spatial velocity of the Hipparcos members of the nearby OB associations already identified by de Zeeuw et al. (1999, Cat. J/AJ/117/354). These stars are first selected using a convergent-point method, and then subjected to further constraints on the proper-motion distribution, magnitude and colour to narrow down the final number of candidate members. Monte Carlo simulations show that the proper-motion distribution, magnitude, and colour constraints remove ~97% of the field stars, while at the same time retain more than 90% of the cluster stars. The procedure has been applied to five nearby associations: the three subgroups of Sco OB2, plus Per OB3 and Cep OB6. In all cases except Cep OB6, we find evidence for new association members fainter than the completeness limit of the Hipparcos Catalogue. However, narrow-band photometry and/or radial velocities are needed to pinpoint the cluster members, and to study their physical characteristics. (1 data file).

  14. Near Infrared Diffuse Interstellar Bands Toward the Cygnus OB2 Association

    NASA Astrophysics Data System (ADS)

    Hamano, Satoshi; Kobayashi, Naoto; Kondo, Sohei; Sameshima, Hiroaki; Nakanishi, Kenshi; Ikeda, Yuji; Yasui, Chikako; Mizumoto, Misaki; Matsunaga, Noriyuki; Fukue, Kei; Yamamoto, Ryo; Izumi, Natsuko; Mito, Hiroyuki; Nakaoka, Tetsuya; Kawanishi, Takafumi; Kitano, Ayaka; Otsubo, Shogo; Kinoshita, Masaomi; Kawakita, Hideyo

    2016-04-01

    We obtained the near-infrared (NIR) high-resolution (R ≡ λ/Δλ ∼ 20,000) spectra of the seven brightest early-type stars in the Cygnus OB2 association for investigating the environmental dependence of diffuse interstellar bands (DIBs). The WINERED spectrograph mounted on the Araki 1.3 m telescope in Japan was used to collect data. All 20 of the known DIBs within the wavelength coverage of WINERED (0.91 < λ < 1.36 μm) were clearly detected along all lines of sight because of their high flux density in the NIR wavelength range and the large extinction. The equivalent widths (EWs) of DIBs were not correlated with the column densities of C2 molecules, which trace the patchy dense component, suggesting that the NIR DIB carriers are distributed mainly in the diffuse component. On the basis of the correlations among the NIR DIBs both for stars in Cyg OB2 and stars observed previously, λλ10780, 10792, 11797, 12623, and 13175 are found to constitute a “family,” in which the DIBs are correlated well over the wide EW range. In contrast, the EW of λ10504 is found to remain almost constant over the stars in Cyg OB2. The extinction estimated from the average EW of λ10504 (AV ∼ 3.6 mag) roughly corresponds to the lower limit of the extinction distribution of OB stars in Cyg OB2. This suggests that λ10504 is absorbed only by the foreground clouds, implying that the carrier of λ10504 is completely destroyed in Cyg OB2, probably by the strong UV radiation field. The different behaviors of the DIBs may be caused by different properties of the DIB carriers.

  15. Astrocyte Leptin Receptor (ObR) and Leptin Transport in Adult-Onset Obese Mice

    PubMed Central

    Pan, Weihong; Hsuchou, Hung; He, Yi; Sakharkar, Amul; Cain, Courtney; Yu, Chuanhui; Kastin, Abba J.

    2008-01-01

    The agouti viable yellow (Avy) spontaneous mutation generates an unusual mouse phenotype of agouti-colored coat and adult-onset obesity with metabolic syndrome. Persistent production of agouti signaling protein in Avy mice antagonizes melanocortin receptors in the hypothalamus. To determine how this disruption of neuroendocrine circuits affects leptin transport across the blood-brain barrier (BBB), we measured leptin influx in Avy and B6 control mice after the development of obesity, hyperleptinemia, and increased adiposity. After iv bolus injection, 125I-leptin crossed the BBB significantly faster in young (2 month old) B6 mice than in young Avy mice or in older (8 month old) mice of either strain. This difference was not observed by in situ brain perfusion studies, indicating the cause being circulating factors, such as elevated leptin levels or soluble receptors. Thus, Avy mice showed peripheral leptin resistance. ObRa, the main transporting receptor for leptin at the BBB, showed no change in mRNA expression in the cerebral microvessels between the age-matched (2 month old) Avy and B6 mice. Higher ObRb mRNA was seen in the Avy microvasculature with unknown significance. Immunofluorescent staining unexpectedly revealed that many of the ObR(+) cells were astrocytes and that the Avy mice showed significantly more ObR(+) astrocytes in the hypothalamus than the B6 mice. Although leptin permeation from the circulation was slower in the Avy mice, the increased ObR expression in astrocytes and increased ObRb mRNA in microvessels suggest the possibility of heightened central nervous system sensitivity to circulating leptin. PMID:18292187

  16. Genistein supplementation prevents weight gain but promotes oxidative stress and inflammation in the vasculature of female obese ob/ob mice.

    PubMed

    Simperova, Anna; Al-Nakkash, Layla; Faust, James J; Sweazea, Karen L

    2016-08-01

    Obesity, a state of chronic low-grade inflammation, is strongly associated with the development of hypertension and diabetes. Superoxide, a free radical elevated in obese individuals, promotes hypertension through scavenging the endogenous vasodilator nitric oxide. The hypothesis was a genistein-enriched diet would promote weight loss and reduce oxidative stress and inflammation in the vasculature of intact female ob/ob mice. Aortas and mesenteric arteries were isolated from female ob/ob mice fed genistein-free (0mg genistein/kg diet; n=6), standard chow (200-300mg genistein/kg diet; n=11) or genistein-enriched (600mg genistein/kg diet; n=9) diets for 4weeks. Sections of isolated vessels were labeled with the superoxide indicator dihydroethidium and fluorescence was measured by confocal microscopy. Protein expression of the inflammatory marker inducible nitric oxide synthase (iNOS) was measured in the perivascular adipose tissue (PVAT) surrounding each vessel and plasma concentrations of superoxide dismutase (SOD) were quantified. Genistein-enriched diet promoted less weight gain compared to animals fed standard chow (P=.008). Standard chow promoted increased superoxide in the aorta (P=.030) and mesenteric arteries (P=.024) compared to a diet devoid of genistein. At all tested concentrations, genistein significantly increased iNOS expression in mesenteric artery PVAT (vs. standard chow, P<.001; vs. genistein-enriched, P=.002) and tended to increase iNOS within the aortic PVAT (standard chow, P=.075) compared to the genistein-free group. Plasma SOD activity was significantly downregulated in genistein-enriched animals as compared to those fed a genistein-free diet (P=.028). In summary, although genistein prevents weight gain, it promotes vascular oxidative stress and inflammation in obese ovarian-intact female mice. PMID:27440533

  17. Long-Acting PASylated Leptin Ameliorates Obesity by Promoting Satiety and Preventing Hypometabolism in Leptin-Deficient Lep(ob/ob) Mice.

    PubMed

    Bolze, Florian; Morath, Volker; Bast, Andrea; Rink, Nadine; Schlapschy, Martin; Mocek, Sabine; Skerra, Arne; Klingenspor, Martin

    2016-01-01

    Body weight loss of Lep(ob/ob) mice in response to leptin is larger than expected from the reduction in energy intake alone, suggesting a thermogenic action of unknown magnitude. We exploited the superior pharmacological properties of a novel long-acting leptin prepared via PASylation to study the contribution of its anorexigenic and thermogenic effects. PASylation, the genetic fusion of leptin with a conformationally disordered polypeptide comprising 600 Pro/Ala/Ser (PAS) residues, provides a superior way to increase the hydrodynamic volume of the fusion protein, thus retarding kidney filtration and extending plasma half-life. Here a single PAS(600)-leptin injection (300 pmol/g) resulted in a maximal weight reduction of 21% 6 days after application. The negative energy balance of 300 kJ/(4 d) was driven by a decrease in energy intake, whereas energy expenditure remained stable. Mice that were food restricted to the same extent showed an energy deficit of only 220 kJ/(4 d) owing to recurring torpor bouts. Therefore, the anorexigenic effect of PAS(600)-leptin contributes 75% to weight loss, whereas the thermogenic action accounts for 25% by preventing hypometabolism. In a second experiment, just four injections of PAS(600)-leptin (100 pmol/g) administered in 5- to 6-day intervals rectified the Lep(ob/ob) phenotype. In total, 16 nmol of PAS(600)-leptin per mouse triggered a weight loss of 43% within 20 days and normalized hypothermia and glucose homeostasis as well as hepatic steatosis. The beneficial properties of PAS(600)-leptin are substantiated by a comparison with previous studies in which approximately 400 nmol (∼25-fold) unmodified leptin was mandatory to achieve similar improvements. PMID:26492472

  18. Explorations of linked editosome domains leading to the discovery of motifs defining conserved pockets in editosome OB-folds

    PubMed Central

    Park, Young-Jun; Hol, Wim G. J.

    2012-01-01

    Trypanosomatids form a group of protozoa which contain parasites of human, animals and plants. Several of these species cause major human diseases, including Trypanosoma brucei which is the causative agent of human African trypanosomiasis, also called sleeping sickness. These organisms have many highly unusual features including a unique U-insertion/deletion RNA editing process in the single mitochondrion. A key multi-protein complex, called the ~20S editosome, or editosome, carries out a cascade of essential RNA-modifying reactions and contains a core of 12 different proteins of which six are the interaction proteins A1 to A6. Each of these interaction proteins comprises a C-terminal OB-fold and the smallest interaction protein A6 has been shown to interact with four other editosome OB-folds. Here we report the results of a “linked OB-fold” approach to obtain a view of how multiple OB-folds might interact in the core of the editosome. Constructs of multiple variants of linked domains in 25 expression and co-expression experiments resulted in 13 soluble multi-OB-fold complexes. In several instances, these complexes were more homogeneous in size than those obtained from corresponding unlinked OB-folds. The crystal structure of A3OB linked to A6 could be elucidated and confirmed the tight interaction between these two OB domains as seen also in our recent complex of A3OB and A6 with nanobodies. In the current crystal structure of A3OB linked to A6, hydrophobic side chains reside in well-defined pockets of neighboring OB-fold domains. When analyzing the available crystal structures of editosome OB-folds, it appears that in five instances “Pocket 1” of A1OB, A3OB and A6 is occupied by a hydrophobic side chain from a neighboring protein. In these three different OB-folds, Pocket 1 is formed by two conserved sequence motifs and an invariant arginine. These pockets might play a key role in the assembly or mechanism of the editosome by interacting with hydrophobic

  19. Analyzing the Effects of Nitrogen Deficiency on the PHB Production of Methylosinus trichosporium OB3b

    NASA Astrophysics Data System (ADS)

    Kyauk, E.

    2011-12-01

    Polyhydroxybutyrate (PHB) is a biodegradable thermoplastic that is produced by various microorganisms. Because of its potential to replace conventional plastics, it has been closely researched in the past few years. Methanotrophic bacteria, bacteria that consume methane, produce this bioplastic when it lacks certain nutrients. The utilization of methane to produce PHB shows much promise as methane is a cheap, plentiful gas. In this study, we observed the methanotroph, Methylosinus trichosporium OB3b , and its yield of PHB in the absence of nitrogen. The optical density of Methylosinus trichosporium OB3b was measured in order to observe cell growth and PHB production patterns over a 48 hour period.

  20. OBS development for long term observation in the Marmara Sea, NW Turkey

    NASA Astrophysics Data System (ADS)

    Takahashi, Narumi; Shimizu, Satoshi; Maekawa, Takuya; Kalafat, Dogan; Pinar, Ali; Citak, Seckin; Kaneda, Yoshiyuki

    2015-04-01

    We have carried out a collaboration study between Japan and Turkey since 2013, which is one of SATREPS projects, "Earthquake and Tsunami Disaster Mitigation in The Marmara Region and Disaster Education in Turkey". The main objective of this project is to reduce risk brought by earthquakes and tsunamis. In particular, the North Anatolian Fault system runs through the Marmara sea and it is expected that the seismic gap exists there according to past seismic studies. The details of seismicity distribution in the Marmara Sea is, however, still insufficient to construct fault model along the active faults. Therefore, we prepare ten ocean bottom seismographs (OBSs) to realize long term observation. We aim to identify size and depth of seismogenic zones using micro seismicity. In addition, we need to cover relative broad area from off-shore Istanbul city to the western end of the Marmara Sea. To clear these conditions, OBS specifications we need are high dynamic range and low instrument noise to observe micro seismicity, low electrical consumption to realize long term observation of over one year, high cost performance to cover the broad area for OBS installation, low cost implementation, and good operability to treat by relatively small number of persons. All items, which are three components velocity sensor, batteries, a recorder, a GPS receiver, a transponder and its transducer to control OBS retrieval, a flasher and a beacon, are installed in the 17 inches glass sphere. The natural frequency of the velocity sensor is 4.5 Hz and the frequency range of our OBS is from 4.5 Hz to 250 Hz. Data sampling is selectable among 100 Hz, 250 Hz and 500 Hz. Because our OBS is deployed by free fall, accuracy of the OBS clock is essentially one of important factors, and it is less than 0.1 ppm. And the resolution of A/D conversion performed on the recorder is 24 bit and we keep the dynamic range of over 135 dB. These data is stored on a semiconductor memory and the capacity is over

  1. Advective transport observations with MODPATH-OBS--documentation of the MODPATH observation process

    USGS Publications Warehouse

    Hanson, R.T.; Kauffman, L.K.; Hill, M.C.; Dickinson, J.E.; Mehl, S.W.

    2013-01-01

    The MODPATH-OBS computer program described in this report is designed to calculate simulated equivalents for observations related to advective groundwater transport that can be represented in a quantitative way by using simulated particle-tracking data. The simulated equivalents supported by MODPATH-OBS are (1) distance from a source location at a defined time, or proximity to an observed location; (2) time of travel from an initial location to defined locations, areas, or volumes of the simulated system; (3) concentrations used to simulate groundwater age; and (4) percentages of water derived from contributing source areas. Although particle tracking only simulates the advective component of conservative transport, effects of non-conservative processes such as retardation can be approximated through manipulation of the effective-porosity value used to calculate velocity based on the properties of selected conservative tracers. This program can also account for simple decay or production, but it cannot account for diffusion. Dispersion can be represented through direct simulation of subsurface heterogeneity and the use of many particles. MODPATH-OBS acts as a postprocessor to MODPATH, so that the sequence of model runs generally required is MODFLOW, MODPATH, and MODPATH-OBS. The version of MODFLOW and MODPATH that support the version of MODPATH-OBS presented in this report are MODFLOW-2005 or MODFLOW-LGR, and MODPATH-LGR. MODFLOW-LGR is derived from MODFLOW-2005, MODPATH 5, and MODPATH 6 and supports local grid refinement. MODPATH-LGR is derived from MODPATH 5. It supports the forward and backward tracking of particles through locally refined grids and provides the output needed for MODPATH_OBS. For a single grid and no observations, MODPATH-LGR results are equivalent to MODPATH 5. MODPATH-LGR and MODPATH-OBS simulations can use nearly all of the capabilities of MODFLOW-2005 and MODFLOW-LGR; for example, simulations may be steady-state, transient, or a combination

  2. Predicting GAIA's Parallax Distance to the Cygnus OB2 Association with Eclipsing Binaries

    NASA Astrophysics Data System (ADS)

    Kiminki, Daniel C.; Kobulnicky, Henry A.; Vargas Álvarez, Carlos A.; Alexander, Michael J.; Lundquist, Michael J.

    2015-10-01

    The Cygnus OB2 Association is one of the nearest and largest collections of massive stars in the Galaxy. Situated at the heart of the “Cygnus X” complex of star-forming regions and molecular clouds, its distance has proven elusive owing to the ambiguous nature of kinematic distances along this ℓ ≃ 80° sightline and the heavy, patchy extinction. In an effort to refine the three-dimensional geometry of key Cygnus X constituents, we have measured distances to four eclipsing double-lined OB-type spectroscopic binaries that are probable members of Cyg OB2. We find distances of 1.33 ± 0.17, 1.32 ± 0.07, 1.44 ± 0.18, and 1.32 ± 0.13 kpc toward MT91 372, MT91 696, CPR2002 A36, and Schulte 3, respectively. We adopt a weighted average distance of 1.33 ± 0.06 kpc. This agrees well with spectrophotometric estimates for the Association as a whole and with parallax measurements of protostellar masers in the surrounding interstellar clouds, thereby linking the ongoing star formation in these clouds with Cyg OB2. We also identify Schulte 3C (O9.5V), a 4″ visual companion to the 4.75 day binary Schulte 3(A+B), as a previously unrecognized Association member.

  3. BOREAS TF-7 SSA-OBS Tower Flux and Meteorological Data

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Huemmrich, Karl (Editor); Pattey, Elizabeth; Desjardins, Raymond L.

    2000-01-01

    The BOREAS TF-7 team collected meteorological data as well as energy, carbon dioxide, water vapor, methane, and nitrous oxide flux data at the BOREAS SSA-OBS site. The data were collected from 24-May to 19-Sep-1994. The data are available in tabular ASCII files.

  4. VizieR Online Data Catalog: Cosmography of OB stars in the solar neighbourhood (Bouy+, 2015)

    NASA Astrophysics Data System (ADS)

    Bouy, H.; Alves, J.

    2016-01-01

    We use the Hipparcos catalogue to revisit the cosmography of OB stars in the solar neighbourhood. Because of the drawbacks mentioned above, we focus on the 3D spatial distribution using modern full 3D data analysis and interactive visualization techniques instead of 2D projections, and refrain from using velocities as a discovery criterion for stellar groups. (1 data file).

  5. Uptake and effect of rare earth elements on gene expression in Methylosinus trichosporium OB3b.

    PubMed

    Gu, Wenyu; Farhan Ul Haque, Muhammad; DiSpirito, Alan A; Semrau, Jeremy D

    2016-07-01

    It is well known that Methylosinus trichosporium OB3b has two forms of methane monooxygenase (MMO) responsible for the initial conversion of methane to methanol, a cytoplasmic (soluble) methane monooxygenase and a membrane-associated (particulate) methane monooxygenase, and that copper strongly regulates expression of these alternative forms of MMO. More recently, it has been discovered that M. trichosporium OB3b has multiple types of the methanol dehydrogenase (MeDH), i.e. the Mxa-type MeDH (Mxa-MeDH) and Xox-type MeDH (Xox-MeDH), and the expression of these two forms is regulated by the availability of the rare earth element (REE), cerium. Here, we extend these studies and show that lanthanum, praseodymium, neodymium and samarium also regulate expression of alternative forms of MeDH. The effect of these REEs on MeDH expression, however, was only observed in the absence of copper. Further, a mutant of M. trichosporium OB3b, where the Mxa-MeDH was knocked out, was able to grow in the presence of lanthanum, praseodymium and neodymium, but was not able to grow in the presence of samarium. Collectively, these data suggest that multiple levels of gene regulation by metals exist in M. trichosporium OB3b, but that copper overrides the effect of other metals by an as yet unknown mechanism. PMID:27190151

  6. Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47T

    SciTech Connect

    Elkins, James G; Lochner, Adriane; Hamilton-Brehm, Scott; Walston Davenport, Karen; Podar, Mircea; Brown, Steven D; Land, Miriam L; Hauser, Loren John; Klingeman, Dawn Marie; Raman, Babu; Goodwin, Lynne A.; Tapia, Roxanne; Meincke, Linda; Detter, J C; Bruce, David; Han, Cliff; Palumbo, Anthony Vito; Cottingham, Robert W; Keller, Martin; Graham, David E

    2010-01-01

    Caldicellulosiruptor obsidiansis OB47T (ATCC BAA-2073; JCM 16842) is an extremely thermophilic, anaerobic bacterium capable of hydrolyzing plant-derived polymers through the expression of multidomain/multifunctional hydrolases. The complete genome sequence reveals a diverse set of carbohydrate-active enzymes and provides further insight into lignocellulosic biomass hydrolysis at high temperatures.

  7. BOREAS TF-9 SSA-OBS Tower Flux, Meteorological, and Soil Temperature Data

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Huemmrich, Karl (Editor); Massheder, Jonathan M.; Moncrieff, John B.; Rayment, Mark B.; Jarvis, Paul G.

    2000-01-01

    The BOREAS TF-9 team collected energy, carbon dioxide, and water vapor flux data at the BOREAS SSA-OBS site during the growing season of 1994 and most of the year for 1996. From the winter of 1995 to 1996, soil temperature data were also collected and provided. The data are available in tabular ASCII files.

  8. An R- and I-band Photometric Variability Survey of the Cygnus OB2 Association

    NASA Astrophysics Data System (ADS)

    Henderson, C. B.; Stanek, K. Z.; Pejcha, O.; Prieto, J. L.

    2011-06-01

    We present a catalog of photometrically variable stars discovered within two 21farcm3 × 21farcm3 fields centered on the Cygnus OB2 association (Cyg OB2). There have hitherto been no deep optical variability studies of Cyg OB2, despite it being replete with early-type massive stars, likely due to the high and variable extinction (up to AV ≈ 20) that permeates much of the region. Here, we provide results of the first variability study with this combination of spatial coverage (~0.5 deg) and photometric depth (R ~ 21 mag). We find 121 stars to be variable in both R and I bands, 115 of them newly discovered. Of the 121 variables, we identify 27 eclipsing binaries and eclipsing-binary candidates, 52 pulsating variables, and 20 potential Herbig Ae/Be stars. Confirming both the status and the cluster membership of the Herbig Ae/Be stars would address the uncertainty regarding the age and star formation history of Cyg OB2. We match our catalog to known variables and binaries in the region, Two Micron All Sky Survey near-IR data, and Chandra X-ray observations to find counterparts to new variables in other wavelengths. Based on observations obtained using the 1.3 m McGraw-Hill telescope at the MDM Observatory.

  9. BOREAS TF-3 NSA-OBS Tower Flux, Meteorological, and Soil Temperature Data

    NASA Technical Reports Server (NTRS)

    Wofsy, Steven; Sutton, Doug; Goulden, Mike; Hall, Forrest G. (Editor); Huemmrich, Karl (Editor)

    2000-01-01

    The BOReal Ecosystem-Atmosphere Study Tower Flux (BOREAS TF-3) team collected tower flux, surface meteorological, and soil temperature data at the BOREAS Northern Study Area-Old Black Spruce (NSA-OBS) site continuously from the March 1994 through October 1996. The data are available in tabular ASCII files.

  10. SPECTROSCOPIC CONFIRMATION OF THE DRAGONFISH ASSOCIATION: THE GALAXY'S MOST LUMINOUS OB ASSOCIATION

    SciTech Connect

    Rahman, Mubdi; Moon, Dae-Sik; Matzner, Christopher D.

    2011-12-20

    Young OB associations with masses greater than 10{sup 4} M{sub Sun} have been inferred to exist in the Galaxy but have largely evaded detection. Recently, a candidate OB association has been identified within the most luminous star-forming complex in the Galaxy, the Dragonfish Nebula. We identify 18 young, massive stars with near-infrared spectroscopy from a sample of 50 members within the candidate OB association, including 15 O-type and 3 luminous blue variables or Wolf-Rayet stars. This number matches the expected yield of massive stars from the candidate association, confirming its existence and ability to power the parent star-forming complex. These results demonstrate the existence of a 10{sup 5} M{sub Sun} OB association, more powerful than any previously known in the Galaxy, comparable in mass only to Westerlund 1. Further, the results also validate the color selection method used to identify the association, adding credence to others discovered in the same way.

  11. AN R- AND I-BAND PHOTOMETRIC VARIABILITY SURVEY OF THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Henderson, C. B.; Stanek, K. Z.; Pejcha, O.; Prieto, J. L.

    2011-06-01

    We present a catalog of photometrically variable stars discovered within two 21.'3 x 21.'3 fields centered on the Cygnus OB2 association (Cyg OB2). There have hitherto been no deep optical variability studies of Cyg OB2, despite it being replete with early-type massive stars, likely due to the high and variable extinction (up to A{sub V} {approx} 20) that permeates much of the region. Here, we provide results of the first variability study with this combination of spatial coverage ({approx}0.5 deg) and photometric depth (R {approx} 21 mag). We find 121 stars to be variable in both R and I bands, 115 of them newly discovered. Of the 121 variables, we identify 27 eclipsing binaries and eclipsing-binary candidates, 52 pulsating variables, and 20 potential Herbig Ae/Be stars. Confirming both the status and the cluster membership of the Herbig Ae/Be stars would address the uncertainty regarding the age and star formation history of Cyg OB2. We match our catalog to known variables and binaries in the region, Two Micron All Sky Survey near-IR data, and Chandra X-ray observations to find counterparts to new variables in other wavelengths.

  12. Cloning of sporulation gene spoOB of Bacillus subtilis and its genetic and biochemical analysis.

    PubMed Central

    Hirochika, H; Kobayashi, Y; Kawamura, F; Saito, H

    1981-01-01

    A specialized transducing phage carrying a sporulation gene (spoOB) was constructed from Bacillus subtilis temperate phage rho 11 by in vitro and in vivo recombinations. Transformation experiments showed that the spoOB gene resides on a 1.4-megadalton fragment generated by EcoRI endonuclease treatment of the phage deoxyribonucleic acid (DNA). Mutants of this phage which lost transducing activity were isolated and used for genetic complementation tests and the analysis of protein(s) coded by the 1.4-megadalton fragment. The spoOB locus was shown to be composed of one cistron. Sodium dodecyl sulfate-polyacrylamide gel analysis of proteins synthesized in ultraviolet-irradiated cells infected with these phages showed that the 1.4-megadalton fragment codes at least one protein, of molecular weight 39,000, which is synthesized in both vegetative and sporulating cells. A cleavage map of the phage DNA was constructed by use of restriction endonucleases, EcoRI, BamHI, and SalI, and the site of integration of the 1.4-megadalton fragment was determined. Expression and function of the spoOB gene are discussed. Images PMID:6260752

  13. Complete Genome Sequence of the Cellulolytic Thermophile Caldicellulosiruptor obsidiansis OB47T▿

    PubMed Central

    Elkins, James G.; Lochner, Adriane; Hamilton-Brehm, Scott D.; Davenport, Karen Walston; Podar, Mircea; Brown, Steven D.; Land, Miriam L.; Hauser, Loren J.; Klingeman, Dawn M.; Raman, Babu; Goodwin, Lynne A.; Tapia, Roxanne; Meincke, Linda J.; Detter, J. Chris; Bruce, David C.; Han, Cliff S.; Palumbo, Anthony V.; Cottingham, Robert W.; Keller, Martin; Graham, David E.

    2010-01-01

    Caldicellulosiruptor obsidiansis OB47T (ATCC BAA-2073, JCM 16842) is an extremely thermophilic, anaerobic bacterium capable of hydrolyzing plant-derived polymers through the expression of multidomain/multifunctional hydrolases. The complete genome sequence reveals a diverse set of carbohydrate-active enzymes and provides further insight into lignocellulosic biomass hydrolysis at high temperatures. PMID:20851897

  14. Properties of interstellar dust in the region of the CEP OB4 association

    NASA Technical Reports Server (NTRS)

    Sudzius, J.

    1989-01-01

    Regional variations in the interstellar extinction law was examined. The region of Cyg was noted among the areas with abnormal interstellar extinction law (IEL). An attempt was made to study interstellar extinction in the region of the Cep OB4 association. The Vilnius seven color photometric system was used for this purpose. The effective wavelengths and their reciprocals for the Vilnius photometric system are presented. This system was applied for the study of variations in the IEL in four regions of the sky: Cyg, Cep, Per, and Mon (Sudzius, 1974). The results of multicolor photometry were combined with the data of Whiteoak (1966) and detailed interstellar extinction curves were derived for Cyg and for Cep-Per-Mon (average) regions in the wavelengths interval of 3000 to 8000 A (Sudzius, 1974). The obtained IEL for the Cep OB4 association does not match any of the laws determined for other regions of the sky. However, it is very close to Cyg OB2 law in the ultraviolet. Results suggest that Cep OB4 IEL have no very broadband structure (VBS) in the interval of wavenumbers 1.84 to 2.15/micron.

  15. The Low-Mass Stellar Content of the Scorpius-Centaurus OB Association

    NASA Technical Reports Server (NTRS)

    Yorke, H.; Kunkel, M.; Brander, W.; Zinnecker, H.; Neuhauser, R.; Schmitt, J.; Mayor, M.; Udry, S.

    2000-01-01

    Based on ROSAT observations and data obtained with ground-based telescopes, we have carried out an extensive study of the low-mass pre-main-sequence population in Upper Scorpius, the youngest subgroup of the Scorpius-Centaurus OB association.

  16. Purification and characterization of oligonucleotide binding (OB)-fold protein from medicinal plant Tinospora cordifolia.

    PubMed

    Amir, Mohd; Haque, Md Anzarul; Wahiduzzaman; Dar, Mohammad Aasif; Islam, Asimul; Ahmad, Faizan; Hassan, Md Imtaiyaz

    2016-01-01

    The oligonucleotide binding fold (OB-fold) is a small structural motif present in many proteins. It is originally named for its oligonucleotide or oligosaccharide binding properties. These proteins have been identified as essential for replication, recombination and repair of DNA. We have successfully purified a protein contains OB-fold from the stem of Tinospora cordifolia, a medicinal plants of north India. Stems were crushed and centrifuged, and fraction obtained at 60% ammonium sulphate was extensively dialyzed and applied to the weak anion exchange chromatography on Hi-Trap DEAE-FF in 50mM Tris-HCl buffer at pH 8.0. Eluted fractions were concentrated and applied to gel filtration column to get pure protein. We observed a single band of 20-kDa on SDS-PAGE. Finally, the protein was identified as OB-fold by MALDI-TOF. The purified OB-fold protein was characterized for its secondary structural elements using circular dichroism (CD) in the far-UV region. Generally the OB-fold has a characteristic feature as five-stranded beta-sheet coiled to form a closed beta- barrel. To estimate its chemical stability, guanidinium chloride-induced denaturation curve was followed by observing changes in the far-UV CD as a function of the denaturant concentration. Analysis of this denaturation curve gave values of 8.90±0.25kcalmol(-1) and 3.78±0.18M for ΔGD° (Gibbs free energy change at 25°C) and Cm (midpoint of denaturation), respectively. To determine heat stability parameters of OB-fold protein, differential scanning calorimetry was performed. Calorimetric values of ΔGD°, Tm (midpoint of denaturation), ΔHm (enthalpy change at Tm), and ΔCp (constant-pressure heat capacity change) are 9.05±0.27kcalmol(-1), 85.2±0,3°C, 105±4kcalmol(-1) and 1.6±0.08kcalmol(-1)K(-1). This is the first report on the isolation, purification and characterization of OB-fold protein from a medicinal plant T. cordifolia. PMID:26613539

  17. Photoionization of the diffuse interstellar medium and galactic halo by OB associtations

    NASA Technical Reports Server (NTRS)

    Dove, James B.; Shull, J. Michael

    1994-01-01

    Assuming smoothly varying H I distributions in te Galactic disk, we have calculated the geometry of diffuse II regions due to OB associations in the Galactic plane. Near the solar circle, OB associations with a Lyman continuum (Lyc) photon luminosity Psi(sub Lyc) = 3.3 x 10(exp 7) cm(exp -2) s(exp -1), produce H II regions that are density bounded in the vertical direction (H II chimneys) allowing Lyc to escape the gaseous disk and penetrate into the Galactic halo. We provide analytic formulae for the Lyc escape fraction as functions of S(sub 0) O-star catalog of Garmany and a new Lyc stellar stellar Lyc stellar flux calibration, we find a production rate of Lyc photons by OB associations within 2.5 kpc of Psi(sub Lyc) = 3.3 x 10(exp 7) cm(exp -2) s(exp -1). Integrating the fraction of Lyc photons that escape the disk over our adopted luminosity function of OB associations, we estimate that approximately 7% of the ionizing photons, or Phi(sub Lyc) = 2.3 x 10(exp 6) cm(exp -2) s(exp -1), escape each side of the H I disk layer and penetrate the diffuse ionized medium ('Reynolds layer'). This flux is sufficient to explain the potoionization of this, although we have not constructed a model for the observed H-alpha emission and pulsar dispersion measures that is fully consistent with the absorption rate of Lyc in the H II layer. Since our quiescent model does not account for the effects of dynamic chimneys and superbubbles, which should enhance Lyc escape, we conclude the O stars are the probable source of ionizing radiation for the Reynolds layer. For a random distribution of OB associations throughout the disk, the Lyc flux is nearly uniform for heights Z is greater than approximately 0.8 kpc above the midplane.

  18. ObsPy: A Python Toolbox for Seismology - Recent Developments and Applications

    NASA Astrophysics Data System (ADS)

    Megies, T.; Krischer, L.; Barsch, R.; Sales de Andrade, E.; Beyreuther, M.

    2014-12-01

    ObsPy (http://www.obspy.org) is a community-driven, open-source project dedicated to building a bridge for seismology into the scientific Python ecosystem. It offersa) read and write support for essentially all commonly used waveform, station, and event metadata file formats with a unified interface,b) a comprehensive signal processing toolbox tuned to the needs of seismologists,c) integrated access to all large data centers, web services and databases, andd) convenient wrappers to legacy codes like libtau and evalresp.Python, currently the most popular language for teaching introductory computer science courses at top-ranked U.S. departments, is a full-blown programming language with the flexibility of an interactive scripting language. Its extensive standard library and large variety of freely available high quality scientific modules cover most needs in developing scientific processing workflows. Together with packages like NumPy, SciPy, Matplotlib, IPython, Pandas, lxml, and PyQt, ObsPy enables the construction of complete workflows in Python. These vary from reading locally stored data or requesting data from one or more different data centers through to signal analysis and data processing and on to visualizations in GUI and web applications, output of modified/derived data and the creation of publication-quality figures.ObsPy enjoys a large world-wide rate of adoption in the community. Applications successfully using it include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. All functionality is extensively documented and the ObsPy tutorial and gallery give a good impression of the wide range of possible use cases.We will present the basic features of ObsPy, new developments and applications, and a roadmap for the near future and discuss the sustainability of our open-source development model.

  19. ObsPy: A Python Toolbox for Seismology and Seismological Observatories

    NASA Astrophysics Data System (ADS)

    Krischer, Lion; Megies, Tobias; Barsch, Robert; Beyreuther, Moritz; Wassermann, Joachim

    2013-04-01

    Python combines the power of a full-blown programming language with the flexibility and accessibility of an interactive scripting language. Its extensive standard library and large variety of freely available high quality scientific modules cover most needs in developing scientific processing workflows. ObsPy extends Python's capabilities to fit the specific needs that arise when working with seismological data. It a) comes with a continuously growing signal processing toolbox that covers most tasks common in seismological analysis, b) provides read and write support for many common waveform, station and event metadata formats and c) enables access to various data centers, webservices and databases to retrieve waveform data and station/event metadata. In combination with mature and free Python packages like NumPy, SciPy, Matplotlib, IPython and PyQt, ObsPy makes it possible to develop complete workflows in Python, ranging from reading locally stored data or requesting data from one or more different data centers via signal analysis and data processing to visualization in GUI and web applications, output of modified/derived data and the creation of publication-quality figures. All functionality is extensively documented and the ObsPy Tutorial and Gallery give a good impression of the wide range of possible use cases. ObsPy is tested and running on Linux, OS X and Windows and comes with installation routines for these systems. ObsPy is developed in a test-driven approach and is available under the GPL/LGPLv3 open source licences. Users are welcome to request help, report bugs, propose enhancements or contribute code via either the user mailing list or the project page on GitHub.

  20. Trigonometric Parallaxes and a Kinematically Adjusted Distance Scale for OB Associations

    NASA Astrophysics Data System (ADS)

    Dambis, A. K.; Mel'Nik, A. M.; Rastorguev, A. S.

    2001-01-01

    By directly comparing the photometric distances of Blaha and Humphreys (1989) (BH) to OB associations and field stars with the corresponding Hipparcos trigonometric parallaxes, we show that the BH distance scale is overestimated, on average, by 10-20%. This result is independently corroborated by applying the rigorous statistical-parallax method and its simplified analog (finding a kinematically adjusted rotation-curve solution from radial velocities and proper motions) to a sample of OB associations. These two methods lead us to conclude that the BH distance scale for OB associations should be shrunk, on average, by 11 +/- 6 and 24 +/- 10 %, respectively. Kinematical parameters have been determined for the system of OB associations: u_0 = 8.2 +/- 1.3 km/s, v_0= 11.9 +/- 1.1 km/s, w_0 = 9.5 +/- 0.9 km/s, sigma_u = 8.2 +/- 1.1 km/s, sigma_v = 5.8 +/- 0.8 km/s, sigma_w = 5.0 +/- 0.8 km/s, Omega_0 = 29.1 +/- 1.0 km/s/kpc, (Omega_0)' = -4.57 +/- 0.20 km/s/kpc^2, and (Omega_0)'' = 1.32 +/- 0.14 km/s/kpc^3. The distance scale for OB associations reduced by 20% matches the short Cepheid distance scale (Berdnikov and Efremov 1985; Sitnik and Mel'nik 1996). Our results are a further argument for the short distance scale in the Universe.

  1. Improving Depression Treatment for Women: Integrating a Collaborative Care Depression Intervention into OB-GYN Care

    PubMed Central

    LaRocco-Cockburn, Anna; Reed, Susan D.; Melville, Jennifer; Croicu, Carmen; Russo, Joan; Inspektor, Michal; Edmondson, Eddie; Katon, Wayne

    2013-01-01

    Background Women have higher rates of depression and often experience depression symptoms during critical reproductive periods, including adolescence, pregnancy, postpartum, and menopause. Collaborative care intervention models for mood disorders in patients receiving care in an OB-GYN clinic setting have not been evaluated. Study design and methodology for a randomized, controlled trial of collaborative care depression management versus usual care in OB-GYN clinics and the details of the adapted collaborative care intervention and model implementation are described in this paper. Methods Women over age 18 years with clinically significant symptoms of depression, as measured by a Patient Health Questionnaire-9 (PHQ-9) score ≥10 and a clinical diagnosis of major depression or dysthymia, were randomized to the study intervention or to usual care and were followed for 18 months. The primary outcome assessed was change over time in the SCL-20 depression scale between baseline and 12 months. Baseline Results 205 women were randomized: 57% white, 20% African American, 9% Asian or Pacific Islander, 7% Hispanic, and 6% Native American. Mean age was 39 years. 4.6% were pregnant and 7.5% were within 12 months postpartum. The majority were single, (52%), and 95% had at least the equivalent of a high school diploma. Almost all patients met DSM IV criteria for major depression (99%) and approximately 33% met criteria for dysthymia. Conclusions An OB-GYN collaborative care team including a social worker, psychiatrist and OB-GYN physician who met weekly and used an electronic tracking system for patients were essential elements of the proposed depression care treatment model described here. Further study of models that improve quality of depression care that are adapted to the unique OB-GYN setting are needed. PMID:23939510

  2. New OB star candidates in the Carina Arm around Westerlund 2 from VPHAS+

    NASA Astrophysics Data System (ADS)

    Mohr-Smith, M.; Drew, J. E.; Barentsen, G.; Wright, N. J.; Napiwotzki, R.; Corradi, R. L. M.; Eislöffel, J.; Groot, P.; Kalari, V.; Parker, Q. A.; Raddi, R.; Sale, S. E.; Unruh, Y. C.; Vink, J. S.; Wesson, R.

    2015-07-01

    O and early B stars are at the apex of galactic ecology, but in the Milky Way, only a minority of them may yet have been identified. We present the results of a pilot study to select and parametrize OB star candidates in the Southern Galactic plane, down to a limiting magnitude of g = 20. A 2 deg2 field capturing the Carina Arm around the young massive star cluster, Westerlund 2, is examined. The confirmed OB stars in this cluster are used to validate our identification method, based on selection from the (u - g, g - r) diagram for the region. Our Markov Chain Monte Carlo fitting method combines VPHAS+ u, g, r, i with published J, H, K photometry in order to derive posterior probability distributions of the stellar parameters log (Teff) and distance modulus, together with the reddening parameters A0 and RV. The stellar parameters are sufficient to confirm OB status while the reddening parameters are determined to a precision of σ(A0) ˜ 0.09 and σ(RV) ˜ 0.08. There are 489 objects that fit well as new OB candidates, earlier than ˜B2. This total includes 74 probable massive O stars, 5 likely blue supergiants and 32 reddened subdwarfs. This increases the number of previously known and candidate OB stars in the region by nearly a factor of 10. Most of the new objects are likely to be at distances between 3 and 6 kpc. We have confirmed the results of previous studies that, at these longer distances, these sight lines require non-standard reddening laws with 3.5 < RV < 4.

  3. The Metal Centers of Particulate Methane Monooxygenase from Methylosinus trichosporium OB3b

    SciTech Connect

    Hakemian,A.; Kondapalli, K.; Telser, J.; Hoffman, B.; Stemmler, T.; Rosenzweig, A.

    2008-01-01

    Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria. The nature of the pMMO active site and the overall metal content are controversial, with spectroscopic and crystallographic data suggesting the presence of a mononuclear copper center, a dinuclear copper center, a trinuclear center, and a diiron center or combinations thereof. Most studies have focused on pMMO from Methylococcus capsulatus (Bath). pMMO from a second organism, Methylosinus trichosporium OB3b, has been purified and characterized by spectroscopic and crystallographic methods. Purified M. trichosporium OB3b pMMO contains 2 copper ions per 100 kDa protomer. Electron paramagnetic resonance (EPR) spectroscopic parameters indicate that type 2 Cu(II) is present as two distinct species. Extended X-ray absorption fine structure (EXAFS) data are best fit with oxygen/nitrogen ligands and reveal a Cu-Cu interaction at 2.52 Angstroms. Correspondingly, X-ray crystallography of M. trichosporium OB3b pMMO shows a dinuclear copper center, similar to that observed previously in the crystal structure of M. capsulatus (Bath) pMMO. There are, however, significant differences between the pMMO structures from the two organisms. A mononuclear copper center present in M. capsulatus (Bath) pMMO is absent in M. trichosporium OB3b pMMO, whereas a metal center occupied by zinc in the M. capsulatus (Bath) pMMO structure is occupied by copper in M. trichosporium OB3b pMMO. These findings extend previous work on pMMO from M. capsulatus (Bath) and provide new insight into the functional importance of the different metal centers.

  4. The metal centers of particulate methane monooxygenase from Methylosinus trichosporium OB3b.

    PubMed

    Hakemian, Amanda S; Kondapalli, Kalyan C; Telser, Joshua; Hoffman, Brian M; Stemmler, Timothy L; Rosenzweig, Amy C

    2008-07-01

    Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria. The nature of the pMMO active site and the overall metal content are controversial, with spectroscopic and crystallographic data suggesting the presence of a mononuclear copper center, a dinuclear copper center, a trinuclear center, and a diiron center or combinations thereof. Most studies have focused on pMMO from Methylococcus capsulatus (Bath). pMMO from a second organism, Methylosinus trichosporium OB3b, has been purified and characterized by spectroscopic and crystallographic methods. Purified M. trichosporium OB3b pMMO contains approximately 2 copper ions per 100 kDa protomer. Electron paramagnetic resonance (EPR) spectroscopic parameters indicate that type 2 Cu(II) is present as two distinct species. Extended X-ray absorption fine structure (EXAFS) data are best fit with oxygen/nitrogen ligands and reveal a Cu-Cu interaction at 2.52 A. Correspondingly, X-ray crystallography of M. trichosporium OB3b pMMO shows a dinuclear copper center, similar to that observed previously in the crystal structure of M. capsulatus (Bath) pMMO. There are, however, significant differences between the pMMO structures from the two organisms. A mononuclear copper center present in M. capsulatus (Bath) pMMO is absent in M. trichosporium OB3b pMMO, whereas a metal center occupied by zinc in the M. capsulatus (Bath) pMMO structure is occupied by copper in M. trichosporium OB3b pMMO. These findings extend previous work on pMMO from M. capsulatus (Bath) and provide new insight into the functional importance of the different metal centers. PMID:18540635

  5. Alterations in Oral [1-14C] 18:1n-9 Distribution in Lean Wild-Type and Genetically Obese (ob/ob) Mice

    PubMed Central

    Wang, Xinxia; Feng, Jie; Yu, Caihua; Shen, Qingwu W.; Wang, Yizhen

    2015-01-01

    Obesity may result from altered fatty acid (FA) disposal. Altered FA distribution in obese individuals is poorly understood. Lean wild-type C57BL/6J and obese C57BL/6Job/ob mice received an oral dose of [1-14C]18:1n-9 (oleic acid), and the radioactivity in tissues was evaluated at various time points. The 14C concentration decreased rapidly in gastrointestinal tract but gradually increased and peaked at 96 h in adipose tissue, muscle and skin in lean mice. The 14C concentration was constant in adipose tissue and muscle of obese mice from 4h to 168h. 14C-label content in adipose tissue was significantly affected by genotype, whereas muscle 14C-label content was affected by genotype, time and the interaction between genotype and time. There was higher total 14C retention (47.7%) in obese mice than in lean mice (9.0%) at 168 h (P<0.05). The 14C concentrations in the soleus and gastrocnemius muscle were higher in obese mice than in lean mice (P<0.05). Perirenal adipose tissue contained the highest 14C content in lean mice, whereas subcutaneous adipose tissue (SAT) had the highest 14C content and accounted for the largest proportion of total radioactivity among fat depots in obese mice. More lipid radioactivity was recovered as TAG in SAT from obese mice than from lean mice (P<0.05). Gene expression suggested acyl CoA binding protein and fatty acid binding protein are important for FA distribution in adipose tissue and muscle. The FA distribution in major tissues was altered in ob/ob mice, perhaps contributing to obesity. Understanding the disparity in FA disposal between lean and obese mice may reveal novel targets for the treatment and prevention of obesity. PMID:25826747

  6. ObsPy: A Python toolbox for Seismology, a Data Center Perspective

    NASA Astrophysics Data System (ADS)

    Beyreuther, Moritz; Barsch, Robert; Krischer, Lion; Megies, Tobias; Behr, Yannik; Wassermann, Joachim

    2010-05-01

    ObsPy: A Python toolbox for seismology (http://www.obspy.org) aims at filling the gap between interactive analysis and automatic data acquistion systems. Automatic batch analysis of continuous data streams or feeding a so far unknown formatted data stream into an acquistion system are two possible applications. Python provides a platform independent, free and open source interpreter language including a large collection of scientific open-source modules thus allowing rapid development of prototype code. ObsPy extends Python by providing the seismologist with basic seismological routines, e.g. MiniSEED, SAC, GSE2 read and write support, various pickers, filters, instrument correction... The data itself is stored in numpy.ndarrays allowing powerful numerical array-programming modules like NumPy (http://numpy.scipy.org) or SciPy (http://scipy.org) to be used. Also SeisComP3 has a Python API which makes use of the previous mentioned modules, thus making it easy to extend SeisComP3 with the help of the ObsPy library. Especially for data centers the ObsPy ArcLink and XSEED modules are of special interest. The ArcLink module makes it possible to easily automatically access the data via ArcLink or for testing the servers functionality. The XSEED module allows to convert data from dataless SEED to XML-SEED and back. The XML-SEED format is very verbose and easy extensible for internal purposes. For "public" distribution the resulting extended XML-SEED can always be converted back to the standard exchange format dataless SEED (loosing the additionally fields). An application of ObsPy is running on the Azores. Here, seismic data are continuous recorded with National Instruments digitizers which are writing data in an binary format every 10s. ObsPy is used to feed the data in EarthWorm and SeisComP3 by decoding the binary format every 30s and appending the new data to a MiniSEED file. The MiniSEED file is continuously scanned by the mseed_scan module of the seedlink server and

  7. Possible Detection of Pi(exp 0)-Decay Gamma-Ray Emission from Cyg OB2 by EGRET

    NASA Technical Reports Server (NTRS)

    Chen, W.; White, R. L.; Bertsch, D.

    1996-01-01

    We report possible detection of pi (exp 0) decay radiation from Cyg OB2, a nearby (1.7 kpc) massive OB star association. The EGRET flux (greater than 100 MeV) maps clearly indicate a point source whose error circle includes both Cyg OB2 and Cyg X-3. We show that Cyg X-3 is unlikely to be the counterpart for the EGRET source, because of the marginal spatial consistency and the lack of the 4.8 hour modulation seen in other high energy emissions from Cyg X-3. If confirmed, this will be the first detection by EGRET of massive stars.

  8. AZ 242, a novel PPARalpha/gamma agonist with beneficial effects on insulin resistance and carbohydrate and lipid metabolism in ob/ob mice and obese Zucker rats.

    PubMed

    Ljung, Bengt; Bamberg, Krister; Dahllöf, Björn; Kjellstedt, Ann; Oakes, Nicholas D; Ostling, Jörgen; Svensson, Lennart; Camejo, Germán

    2002-11-01

    Abnormalities in fatty acid (FA) metabolism underlie the development of insulin resistance and alterations in glucose metabolism, features characteristic of the metabolic syndrome and type 2 diabetes that can result in an increased risk of cardiovascular disease. We present pharmacodynamic effects of AZ 242, a novel peroxisome proliferator activated receptor (PPAR)alpha/gamma agonist. AZ 242 dose-dependently reduced the hypertriglyceridemia, hyperinsulinemia, and hyperglycemia of ob/ob diabetic mice. Euglycemic hyperinsulinemic clamp studies showed that treatment with AZ 242 (1 micromol/kg/d) restored insulin sensitivity of obese Zucker rats and decreased insulin secretion. In vitro, in reporter gene assays, AZ 242 activated human PPARalpha and PPARgamma with EC(50) in the micro molar range. It also induced differentiation in 3T3-L1 cells, an established PPARgamma effect, and caused up-regulation of liver fatty acid binding protein in HepG-2 cells, a PPARalpha-mediated effect. PPARalpha-mediated effects of AZ 242 in vivo were documented by induction of hepatic cytochrome P 450-4A in mice. The results indicate that the dual PPARalpha/gamma agonism of AZ 242 reduces insulin resistance and has beneficial effects on FA and glucose metabolism. This effect profile could provide a suitable therapeutic approach to the treatment of type 2 diabetes, metabolic syndrome, and associated vascular risk factors. PMID:12401884

  9. Intravenous Mycobacterium Bovis Bacillus Calmette-Guérin Ameliorates Nonalcoholic Fatty Liver Disease in Obese, Diabetic ob/ob Mice

    PubMed Central

    Inafuku, Masashi; Matsuzaki, Goro; Oku, Hirosuke

    2015-01-01

    Inflammation and immune response profoundly influence metabolic syndrome and fatty acid metabolism. To analyze influence of systemic inflammatory response to metabolic syndrome, we inoculated an attenuated vaccine strain of Mycobacterium bovis Bacillus Calmette–Guérin (BCG) into leptin-deficient ob/ob mice. BCG administration significantly decreased epididymal white adipose tissue weight, serum insulin levels, and a homeostasis model assessment of insulin resistance. Serum high molecular weight (HMW) adiponectin level and HMW/total adiponectin ratio of the BCG treated mice were significantly higher than those of control mice. Hepatic triglyceride accumulation and macrovesicular steatosis were markedly alleviated, and the enzymatic activities and mRNA levels of lipogenic-related genes in liver were significantly decreased in the BCG injected mice. We also exposed human hepatocellular carcinoma HepG2 cells to high levels of palmitate, which enhanced endoplasmic reticulum stress-related gene expression and impaired insulin-stimulated Akt phosphorylation (Ser473). BCG treatment ameliorated both of these detrimental events. The present study therefore suggested that BCG administration suppressed development of nonalcoholic fatty liver disease, at least partly, by alleviating fatty acid-induced insulin resistance in the liver. PMID:26039731

  10. Ara OB1 - A stellar association formed by the action of an energetic event?

    NASA Technical Reports Server (NTRS)

    Arnal, E. M.; Cersosimo, J. C.; May, J.; Bronfman, L.

    1987-01-01

    Neutral hydrogen (at the wavelength of 21 cm) and carbon monoxide (at 2.6 mm) line observations of the interstellar medium in the neighborhood of the association Ara OB1 are reported. The observed H I distribution indicates the presence of an expanding structure. The total H I mass associated with such feature amounts to 4800 solar masses, and its momentum and kinetic energy are about 40,000 solar mass km/s and 3 x 10 to the 48th erg, respectively. The H I structure, 42 pc in diameter, expands at a speed of 10 km/s. A possible origin for the expanding H I structure, and a genetic link between such structure and Ara OB1 are proposed.

  11. Discharges and yields of suspended sediment in the Ob' and Yenisey Rivers of Siberia

    USGS Publications Warehouse

    Bobrovitskaya, N.N.; Zubkova, C.; Meade, R.H.

    1996-01-01

    The northward-flowing rivers of Siberia deliver immense quantities of water but only relatively small quantities of sediment to the Arctic Ocean. The relatively low delivery of sediment to the ocean by these rivers is explained by the large areas of forest and swamp in their basins. In the Ob' River, sediment yields tend to increase between the headwaters and Kamen' na Obi. Further downstream, sediment yields gradually decrease. Near the mouth of the Ob' River, at Salekhard, sediment yield has remained constant at about 5.3 t km-2 year-1. In the lower Yenisey River, the already small natural sediment yield of 5.4 t km-2 year-1 has been decreased several fold by the construction of massive reservoirs on the main stem and on the tributary Angara River, and presently equals 1.8 t km-2 year-1.

  12. BOREAS TGB-8 Photosynthetic Rate Data over the SSA-OBS and the SSA-OJP

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Conrad, Sara K. (Editor); Lerdau, Manuel

    2000-01-01

    The BOREAS TGB-8 team collected data to investigate the controls over NMHC fluxes from boreal forest tree species. This data set includes measurements of photosynthetic rates at mature jack pine and black spruce sites. The data were collected at the OJP and OBS tower flux locations in the BOREAS SSA. These areas contained mature stands of jack pine and black spruce and were the focal sites in the BOREAS program for studies of biosphere/atmosphere exchange from these two habitat types. The OBS site is situated in a black spruce/sphagnum bog with the largest trees 155 years old and 10-15 m tall. The OJP site is in a jack pine forest, 80 to 120 years old, which lies on a sandy bench of glacial outwash with the largest tree standing 15 m tall. Temporally, the data cover the period of 24-May-1994 to 19-Sep-1994. The data are stored in tabular ASCII files.

  13. Proposal of a multi-layer network architecture for OBS/GMPLS network interworking

    NASA Astrophysics Data System (ADS)

    Guo, Hongxiang; Tsuritani, Takehiro; Yin, Yawei; Otani, Tomohiro; Wu, Jian

    2007-11-01

    In order to enable the existing optical circuit switching (OCS) network to support both wavelength and subwavelength granularities, this paper proposes overlay-based multi-layer network architecture for interworking the generalized multi-protocol label switching (GMPLS) controlled OCS network with optical burst switching (OBS) networks. A dedicated GMPLS border controller with necessary GMPLS extensions, including group label switching path (LSP) provisioning, node capability advertisement, and standard wavelength label as well as wavelength availability advertisement, is introduced in this multi-layer network to enable a simple but flexible interworking operation. The feasibility of this proposal is experimentally confirmed by demonstrating an OBS/GMPLS testbed, in which the extended node capability advertisement and group LSP functions successfully enabled the burst header packet (BHP) and data burst (DB) to transmit over a GMPLS-controlled transparent OCS network.

  14. BOREAS TF-3 Automated Chamber CO2 Flux Data from the NSA-OBS

    NASA Technical Reports Server (NTRS)

    Goulden, Michael L.; Crill, Patrick M.; Hall, Forrest G. (Editor); Conrad, Sara (Editor)

    2000-01-01

    The BOReal Ecosystem Atmosphere Study Tower Flux (BOREAS TF-3) and Trace Gas Biogeochemistry (TGB-1) teams collected automated CO2 chamber flux data in their efforts to fully describe the CO2 flux at the Northern Study Area-Old Black Spruce (NSA-OBS) site. This data set contains fluxes of CO2 at the NSA-OBS site measured using automated chambers. In addition to reporting the CO2 flux, it reports chamber air temperature, moss temperature, and light levels during each measurement. The data set covers the period from 23-Sep-1995 through 26-Oct-1995 and from 28-May-1996 through 21-Oct-1996. The data are stored in tabular ASCII files.

  15. Reflections on the Canadian MORE(OB) obstetrical risk management programme.

    PubMed

    Milne, J K; Walker, David E; Vlahaki, Dean

    2013-08-01

    In, 2001, the Patient Safety Division of the Society of Obstetricians and Gynaecologists of Canada initiated and championed a new program to improve patient safety performance in Canadian hospital obstetric units. This new program was developed under the banner of Managing Obstetrical Risk Efficiently and called the MORE(OB) Programme The MORE(OB) Programme was first piloted in Canadian hospitals at the beginning of May 2002 and, by mid 2004, 33 pilot sites had been implemented. In autumn 2004, this program embarked on a national launch. In 2007, the Society of Obstetricians and Gynaecologists of Canada collaborated with the Healthcare Insurance Reciprocal of Canada to form Salus Global Corporation. The birth of this corporate entity embraced the support of rapid expansion of the program within and outside of Canada. This collaboration also enabled innovation and implementation of safety programs beyond the obstetric discipline. PMID:23642351

  16. Batch conversion of methane to methanol using Methylosinus trichosporium OB3b as biocatalyst.

    PubMed

    Hwang, In Yeub; Hur, Dong Hoon; Lee, Jae Hoon; Park, Chang-Ho; Chang, In Seop; Lee, Jin Won; Lee, Eun Yeol

    2015-03-01

    Recently, methane has attracted much attention as an alternative carbon feedstock since it is the major component of abundant shale and natural gas. In this work, we produced methanol from methane using whole cells of Methylosinus trichosporium OB3b as the biocatalyst. M. trichosporium OB3b was cultured on NMS medium with a supply of 7:3 air/methane ratio at 30°C. The optimal concentrations of various methanol dehydrogenase inhibitors such as potassium phosphate and EDTA were determined to be 100 and 0.5 mM, respectively, for an efficient production of methanol. Sodium formate (40 mM) as a reducing power source was added to enhance the conversion efficiency. A productivity of 49.0 mg/l·h, titer of 0.393 g methanol/l, and conversion of 73.8% (mol methanol/mol methane) were obtained under the optimized batch condition. PMID:25563419

  17. Signals embedded in the OBS records, in light of Gabor Spectral Analysis

    NASA Astrophysics Data System (ADS)

    Chang, T.; Wang, Y.; Chang, C.; Lee, C.

    2005-12-01

    Since the last decades, seismological survey has been expanded to marine area, with goal of making up the deficiency of seismogenic study outside the land. Although teleseismic data can resolve plate boundaries location and certain seismic parameters for great earthquake, local seismogenic frame can be merely emerged by the seismic network in situ. The Ocean Bottom Seismometer (OBS), therefore, is developing for this kind of purpose and becoming an important facility for seismological study. This work introduces a synthesized spectral method to analyze the seismograms recorded by 15 OBS deployed at the Okinawa trough in 14 days (Nov. 19 ~Dec. 2, 2003). Geological background of Okinawa trough is well known to correspond with the back-arc spreading in the regime of the Philippine Sea plate subducting northward beneath the Eurasia plate. As the complex affections at sea bottom, for instance, strong current, slope slumping, turbidite flow, and even sea animal attack, the OBS seismogram show a rather noisy sequence in comparison with the record on land. However, hundreds of tectonic earthquake can be extracted from such noisy records (done by Drs. Lin and Sibuet). Our job is to sort out the signals with the distinguishable sources by means of a systematically spectral analysis. The continuous wavelet transform and short-term Fourier transform, both taking Gaussian function as kernel, are synthesized as the Gabor transform in data process. The use of a limited Gaussian window along time axis with negligible low frequency error can largely enhance the stability of discrete Fourier spectrum. With a proper window factor selection, the Gabor transform can improve the resolution of spectrogram in time domain. We have converted the OBS records into spectrograms to detect the variation of signal causes. Up-to-date, some tremors signals and strong current oscillations have been told apart from these continuous records with varied frequency composing. We anticipate the further

  18. Escape fraction of ionizing photons during reionization: Effects due to supernova feedback and runaway ob stars

    SciTech Connect

    Kimm, Taysun; Cen, Renyue

    2014-06-20

    The fraction of hydrogen ionizing photons escaping from galaxies into the intergalactic medium is a critical ingredient in the theory of reionization. We use two zoomed-in, high-resolution (4 pc), cosmological radiation hydrodynamic simulations with adaptive mesh refinement to investigate the impact of two physical mechanisms (supernova, SN, feedback, and runaway OB stars) on the escape fraction (f {sub esc}) at the epoch of reionization (z ≥ 7). We implement a new, physically motivated SN feedback model that can approximate the Sedov solutions at all (from the free expansion to snowplow) stages. We find that there is a significant time delay of about ten million years between the peak of star formation and that of escape fraction, due to the time required for the build-up and subsequent destruction of the star-forming cloud by SN feedback. Consequently, the photon number-weighted mean escape fraction for dwarf galaxies in halos of mass 10{sup 8}-10{sup 10.5} M {sub ☉} is found to be 〈f{sub esc}〉∼11%, although instantaneous values of f {sub esc} > 20% are common when star formation is strongly modulated by the SN explosions. We find that the inclusion of runaway OB stars increases the mean escape fraction by 22% to 〈f{sub esc}〉∼14%. As SNe resulting from runaway OB stars tend to occur in less dense environments, the feedback effect is enhanced and star formation is further suppressed in halos with M{sub vir}≳10{sup 9} M{sub ⊙} in the simulation with runaway OB stars compared with the model without them. While both our models produce enough ionizing photons to maintain a fully ionized universe at z ≤ 7 as observed, a still higher amount of ionizing photons at z ≥ 9 appears necessary to accommodate the high observed electron optical depth inferred from cosmic microwave background observations.

  19. Influence on spacecraft control systems of evolving O/B technology and standards

    NASA Astrophysics Data System (ADS)

    Creasey, Richard

    1990-10-01

    Analysis of the operation requirements of future spacecraft show that past approaches are not sufficient. The use of on board (O/B) technology and the development of the specific standards are proposed in order to meet those increasing requirements. The increase in on board decision making capability in spacecraft is discussed. Standardization of spacecraft operations and the impact on present and future ESA standards by this trend are considered.

  20. Escape Fraction of Ionizing Photons during Reionization: Effects due to Supernova Feedback and Runaway OB Stars

    NASA Astrophysics Data System (ADS)

    Kimm, Taysun; Cen, Renyue

    2014-06-01

    The fraction of hydrogen ionizing photons escaping from galaxies into the intergalactic medium is a critical ingredient in the theory of reionization. We use two zoomed-in, high-resolution (4 pc), cosmological radiation hydrodynamic simulations with adaptive mesh refinement to investigate the impact of two physical mechanisms (supernova, SN, feedback, and runaway OB stars) on the escape fraction (f esc) at the epoch of reionization (z >= 7). We implement a new, physically motivated SN feedback model that can approximate the Sedov solutions at all (from the free expansion to snowplow) stages. We find that there is a significant time delay of about ten million years between the peak of star formation and that of escape fraction, due to the time required for the build-up and subsequent destruction of the star-forming cloud by SN feedback. Consequently, the photon number-weighted mean escape fraction for dwarf galaxies in halos of mass 108-1010.5 M ⊙ is found to be \\langle{f_esc}\\rangle\\sim 11%, although instantaneous values of f esc > 20% are common when star formation is strongly modulated by the SN explosions. We find that the inclusion of runaway OB stars increases the mean escape fraction by 22% to \\langle{f_esc}\\rangle\\sim 14%. As SNe resulting from runaway OB stars tend to occur in less dense environments, the feedback effect is enhanced and star formation is further suppressed in halos with {{M}_vir}\\gtrsim 10^9\\,{{M}_\\odot } in the simulation with runaway OB stars compared with the model without them. While both our models produce enough ionizing photons to maintain a fully ionized universe at z <= 7 as observed, a still higher amount of ionizing photons at z >= 9 appears necessary to accommodate the high observed electron optical depth inferred from cosmic microwave background observations.

  1. The large scale view of the young stellar populations in the Orion OB1 Association

    NASA Astrophysics Data System (ADS)

    Briceno, Cesar; Calvet, Nuria

    2016-06-01

    The Orion OB1 association, at ~400 pc and with a wide range of ages (~1-10 Myr) and environmental conditions, is an ideal place to look at how stars form, first evolve and disperse among the general population of field stars. Also to study disk dispersal and the duration of the planet formation phase.However, despite spanning nearly 200 deg2 on the sky, almost all we know about Orion comes from studies of a limited fraction of the entire region, mostly of the youngest objects (~<1 Myr) in the A and B molecular clouds and the ~3 Myr old sigma Ori cluster.We will present here the results of our 180 sq deg photometric multi-epoch survey across the Orion OB1 association, using the known variability of T Tauri stars to pick them among the general field population, and following with spectroscopy to confirm members and characterize them.The ~2000 newly identified young low-mass stars are mostly located away from the molecular clouds, across tens of sq. deg. in the Orion OB1a and OB1b sub-associations, with ages in the range ~4-10 Myr. But within this general population we identify a significant fraction concentrated in distinct overdensities, most notably the ~7 Myr old 25 Orionis cluster. These stellar aggregates point to a previously unknown degree of substructure that has survived the dissipation of the parent molecular clouds. We also find that the Orion Nebula Cluster is surrounded by a few sq.deg. halo of young stars, as has been suggested by recent sudies.

  2. A Study of Interstellar Ultraviolet Extinction in OB Associations and Star Forming Regions

    NASA Astrophysics Data System (ADS)

    Aiello, S.; Barsella, B.; Chlewicki, G.; Greenberg, J. M.; Patriarchi, P.; Perinotto, M.

    The authors studied the interstellar UV extinction towards 115 stars, selected from the IUE data bank. The complete catalogue, including extinction data for the whole sample as well as the reduction procedure and error analysis, is in preparation (Aiello et al., 1986). Here the authors present the results referring to two regions (Carina and Ophiuchus Complexes) where the interstellar medium is disturbed by the effects of recent and ongoing star formation. For comparison purposes, the extinction towards Cas OB6 is also reported.

  3. Improvements to the gridding of precipitation data across Europe under the E-OBS scheme

    NASA Astrophysics Data System (ADS)

    Cornes, Richard; van den Besselaar, Else; Jones, Phil; van der Schrier, Gerard; Verver, Ge

    2016-04-01

    Gridded precipitation data are a valuable resource for analyzing past variations and trends in the hydroclimate. Such data also provide a reference against which model simulations may be driven, compared and/or adjusted. The E-OBS precipitation dataset is widely used for such analyses across Europe, and is particularly valuable since it provides a spatially complete, daily field across the European domain. In this analysis, improvements to the E-OBS precipitation dataset will be presented that aim to provide a more reliable estimate of grid-box precipitation values, particularly in mountainous areas and in regions with a relative sparsity of input station data. The established three-stage E-OBS gridding scheme is retained, whereby monthly precipitation totals are gridded using a thin-plate spline; daily anomalies are gridded using indicator kriging; and the final dataset is produced by multiplying the two grids. The current analysis focuses on improving the monthly thin-plate spline, which has overall control on the final daily dataset. The results from different techniques are compared and the influence on the final daily data is assessed by comparing the data against gridded country-wide datasets produced by various National Meteorological Services

  4. On the nature of high reddening of Cygnus OB2 #12 hypergiant

    NASA Astrophysics Data System (ADS)

    Maryeva, O. V.; Chentsov, E. L.; Goranskij, V. P.; Dyachenko, V. V.; Karpov, S. V.; Malogolovets, E. V.; Rastegaev, D. A.

    2016-05-01

    To explain the nature of the high reddening (AV ≃ 10 mag) towards one of the most luminous stars in the Galaxy - Cyg OB2 #12 (B5 Ia-0), also known as MT304, we carried out spectrophotometric observations of 24 stars located in its vicinity. We included five new B-stars among the members of Cygnus OB2, and for five more photometrically selected stars we spectroscopically confirmed their membership. We constructed the map of interstellar extinction within 2.5 arcmin radius and found that interstellar extinction increases towards MT304. According to our results the most reddened OB-stars in the association after MT304 are J203240.35+411420.1 and J203239.90+411436.2, located about 15 arcsec away from it. Interstellar extinction towards these stars is about 9 mag. The increase of reddening towards MT304 suggests that the reddening excess may be caused by the circumstellar shell ejected by the star during its evolution. This shell absorbs 1 mag, but its chemical composition and temperature are unclear. We also report the detection of a second component of MT304, and discovery of an even fainter third component, based on data of speckle interferometric observations taken with the Russian 6-m telescope.

  5. Imaging beneath an opaque basaltic layer using densely sampled wide-angle OBS data

    SciTech Connect

    Samson, C.; Barton, P.J.; Karwatowski, J.

    1995-05-01

    A combined reflection/refraction (wide-angle) seismic survey was conducted on the continental shelf north-west of Britain, using a conventional streamer with an air gun source, and static ocean-bottom seismometers (OBS) to record wide-angle energy. The shallow structure down to a basaltic layer was reasonably well imaged on the stacked reflection section. The basalts, however, proved to be opaque to the conventional reflection method and prevented the imaging of deeper horizons, where an important velocity inversion was anticipated. This paper reports on the processing, modeling and interpretation of the densely sampled wide-angle OBS data that were coincident with the reflection profile. Eleven OBS instruments were deployed along a 75 km line and recorded signal from a powerful 149 liter (9100 in.{sup 3}) air gun array fired every 50 m. Data processing was performed using a standard industrial reflection seismic software package prior to first-arrival picking. Processing steps included geometry definition, trace summation and display of the data using various scaling algorithms. An initial model was constructed from 1D velocity-time profiles digitized every 4 km along the stacked section. First arrival travel time modeling rapidly converged to a detailed model of the structure of the top 5 km of the crust. Modeling revealed the existence of a buried low-velocity Mesozoic sedimentary basin, of a prominent basement horst and of a normal fault penetrating to the basement.

  6. A model for the infrared emission from an OB star cluster environment

    NASA Technical Reports Server (NTRS)

    Leisawitz, D.

    1990-01-01

    Researchers developed an interactive radiative transfer code that predicts the infrared emission from an HII region containing diffuse ionized and atomic gas and dense molecular clouds. This model complements the investigation of the redistribution of OB star luminosity in the interstellar medium (Leisawitz and Hauser 1988, Ap. J., 332, 954). The model can be used as a diagnostic tool to probe the radiation field and matter density in an HII region, place constraints on the proximity and orientation of an illuminated molecular cloud with respect to the ionizing stars, test for the presence of small, transiently heated dust grains, and determine whether the dust-to-gas ratio is normal. Predictions of the model agree qualitatively and quantitatively with observations of blister-type HII regions ionized by well-studied OB clusters in which the distribution of dense neutral material is known. This is illustrated by a model for Infrared Astronomy Satellite (IRAS) observations of the region around NGC 7380 (S142). Researchers plan to use the model in a survey of regions of massive star formation in the outer Galaxy to study OB stars embedded to various degrees in their parental molecular clouds.

  7. Diacylglycerol production induced by growth hormone in Ob1771 preadipocytes arises from phosphatidylcholine breakdown

    SciTech Connect

    Catalioto, R.M.; Ailhaud, G.; Negrel, R. )

    1990-12-31

    Growth Hormone has recently been shown to stimulate the formation of diacylglycerol in Ob1771 mouse preadipocyte cells without increasing inositol lipid turnover. Addition of growth hormone to Ob1771 cells prelabelled with ({sup 3}H)glycerol or ({sup 3}H)choline led to a rapid, transient and stoechiometric formation of labelled diacylglycerol and phosphocholine, respectively. In contrast, no change was observed in the level of choline and phosphatidic acid whereas the release of water-soluble metabolites in ({sup 3}H)ethanolamine prelabelled cells exposed to growth hormone was hardly detectable. Stimulation by growth hormone of cells prelabelled with (2-palmitoyl 9, 10 ({sup 3}H))phosphatidylcholine also induced the production of labelled diacyglycerol. Pertussis toxin abolished both diacylglycerol and phosphocholine formation induced by growth hormone. It is concluded that growth hormone mediates diacylglycerol production in Ob1771 cells by means of phosphatidylcholine breakdown involving a phospholipase C which is likely coupled to the growth hormone receptor via a pertussis toxin-sensitive G-protein.

  8. CLUSTERING BETWEEN HIGH-MASS X-RAY BINARIES AND OB ASSOCIATIONS IN THE MILKY WAY

    SciTech Connect

    Bodaghee, A.; Tomsick, J. A.; Rodriguez, J.

    2012-01-10

    We present the first direct measurement of the spatial cross-correlation function of high-mass X-ray binaries (HMXBs) and active OB star-forming complexes in the Milky Way. This result relied on a sample containing 79 hard X-ray-selected HMXBs and 458 OB associations. Clustering between the two populations is detected with a significance above 7{sigma} for distances <1 kpc. Thus, HMXBs closely trace the underlying distribution of the massive star-forming regions that are expected to produce the progenitor stars of HMXBs. The average offset of 0.4 {+-} 0.2 kpc between HMXBs and OB associations is consistent with being due to natal kicks at velocities of the order of 100 {+-} 50 km s{sup -1}. The characteristic scale of the correlation function suggests an average kinematical age (since the supernova phase) of {approx}4 Myr for the HMXB population. Despite being derived from a global view of our Galaxy, these signatures of HMXB evolution are consistent with theoretical expectations as well as observations of individual objects.

  9. An unidentified TeV source in the vicinity of Cygnus OB2

    NASA Astrophysics Data System (ADS)

    Aharonian, F.; Akhperjanian, A.; Beilicke, M.; Bernlöhr, K.; Börst, H.; Bojahr, H.; Bolz, O.; Coarasa, T.; Contreras, J.; Cortina, J.; Denninghoff, S.; Fonseca, V.; Girma, M.; Götting, N.; Heinzelmann, G.; Hermann, G.; Heusler, A.; Hofmann, W.; Horns, D.; Jung, I.; Kankanyan, R.; Kestel, M.; Kettler, J.; Kohnle, A.; Konopelko, A.; Kornmeyer, H.; Kranich, D.; Krawczynski, H.; Lampeitl, H.; Lopez, M.; Lorenz, E.; Lucarelli, F.; Magnussen, N.; Mang, O.; Meyer, H.; Milite, M.; Mirzoyan, R.; Moralejo, A.; Ona, E.; Panter, M.; Plyasheshnikov, A.; Prahl, J.; Pühlhofer, G.; Rauterberg, G.; Reyes, R.; Rhode, W.; Ripken, J.; Röhring, A.; Rowell, G. P.; Sahakian, V.; Samorski, M.; Schilling, M.; Schröder, F.; Siems, M.; Sobzynska, D.; Stamm, W.; Tluczykont, M.; Völk, H. J.; Wiedner, C. A.; Wittek, W.; Uchiyama, Y.; Takahashi, T.; HEGRA Collaboration

    2002-10-01

    Deep observation ( ~ 113 hrs) of the Cygnus region at TeV energies using the HEGRA stereoscopic system of air Čerenkov telescopes has serendipitously revealed a signal positionally inside the core of the OB association Cygnus OB2, at the edge of the 95% error circle of the EGRET source 3EG J2033+4118, and ~ 0.5o north of Cyg X-3. The source centre of gravity is RA alphaJ2000: 20h 32m 07s+/- 9.2sstat +/-2.2ssys, Dec deltaJ2000: +41o 30' 30''+/- 2.0'stat +/- 0.4'sys. The source is steady, has a post-trial significance of +4.6sigma , indication for extension with radius 5.6' at the ~ 3sigma level, and has a differential power-law flux with hard photon index of -1.9 +/-0.3stat +/-0.3sys. The integral flux above 1 TeV amounts ~ 3% that of the Crab. No counterpart for the TeV source at other wavelengths is presently identified, and its extension would disfavour an exclusive pulsar or AGN origin. If associated with Cygnus OB2, this dense concentration of young, massive stars provides an environment conducive to multi-TeV particle acceleration and likely subsequent interaction with a nearby gas cloud. Alternatively, one could envisage gamma -ray production via a jet-driven termination shock.

  10. Galactic OB associations in the northern Milky Way Galaxy. I - Longitudes 55 deg to 150 deg

    NASA Technical Reports Server (NTRS)

    Garmany, C. D.; Stencel, R. E.

    1992-01-01

    The literature on all OB associations was reviewed, and their IRAS point source content was studied, between galactic longitude 55 and 150 deg. Only one third of the 24 associations listed by Ruprecht et al. (1981) have been the subject of individual studies designed to identify the brightest stars. Distances to all of these were recomputed using the method of cluster fitting of the B main sequence stars, which makes it poossible to reexamine the absolute magnitude calibration of the O stars, as well as for the red supergiant candidate stars. Also examined was the composite HR diagram for these associations. Associations with the best defined main sequences, which also tend to contain very young clusters, referred to here as OB clusters, have extremely few evolved B and A or red supergiants. Associations with poorly defined main sequences and few OB clusters have many more evolved stars. They also show an effect in the upper HR diagram referred to as a ledge by Fitzpatrick and Garmany (1990) in similar data for the Large Magellanic Cloud. It is suggested that the differences in the associations are not just observational selection effects but represent real differences in age and formation history.

  11. Gas Hydrate at the Formosa Ridge in the Northernmost SCS Imaged from OBS Data

    NASA Astrophysics Data System (ADS)

    Deng, J.; Wang, T. K.; Chen, T.; Chi, W.; Lin, S.

    2013-12-01

    Gas hydrate at the Formosa Ridge off SW Taiwan in the passive margin of the northernmost South China Sea (SCS) was investigated by using 6 Taiwan ocean-bottom seismometers (OBS) and 12 German OBS along several P-cable profiles shooting by GI guns from the German research vessel SONNE in 2013. Firstly, initial models were built based on the P-cable profiles. Secondly, the reflected, refracted and head-wave arrivals, propagating from the shallow sedimentary layers, hydrate- and gas-bearing sediments, were picked from OBS data to invert the initial models. Finally, we obtain the P-wave velocity models by reducing the travel-time errors. Based on the P-wave velocity models, the depth of bottom-simulating reflector (BSR) beneath the Formosa Ridge in the passive margin of the northernmost SCS off SW Taiwan is about 400 meter below the sea floor. Furthermore, P-wave velocities of hydrate- and gas-bearing sediments are about 2000 m/s and 1450 m/s, respectively. We suggested that normal faults have provided conduits for gas migrated upward so that hydrate was accumulated above the BSR at the anticline of the Formosa Ridge off SW Taiwan in the passive margin of the SCS.

  12. Leptin and ObRa/MEK signalling in mouse oocyte maturation and preimplantation embryo development.

    PubMed

    Ye, Yinghui; Kawamura, Kazuhiro; Sasaki, Mitsue; Kawamura, Nanami; Groenen, Peter; Sollewijn Gelpke, Maarten D; Kumagai, Jin; Fukuda, Jun; Tanaka, Toshinobu

    2009-08-01

    Recent studies indicate that LH stimulates production of ovarian paracrine factors that induce meiosis of the oocyte. DNA microarray analyses of ovarian transcripts were performed in mice and major increases of a short isoform of leptin receptor, ObRa, were identified by the preovulatory LH/human chorionic gonadotrophin (HCG) surge. In oocytes, the level of ObRa transcripts was increased shortly after HCG stimulation, whereas the level of ObRb transcripts was not changed. Leptin was produced by cumulus, granulosa, theca and interstitial cells of ovaries and its transcript level was not regulated during gonadotrophin treatment. Treatment with leptin promoted germinal vesicle breakdown (GVBD) in oocytes within preovulatory follicles, and enhance first polar body extrusion in both cumulus-oocyte complexes and denuded oocytes. The leptin-promoted GVBD and first polar body extrusion were blocked by a mitogen-activated protein kinase extracellular signal regulated kinase kinases (MEK)1/2 inhibitor, U0126, but not its inactive analogue U0124. Furthermore, leptin promoted fertilization of oocytes and the in-vitro development of zygotes to preimplantation embryos. These findings suggest paracrine roles of leptin in the enhancement of nuclear maturation of oocytes through MEK1/2 signalling, and in the promotion of cytoplasmic maturation essential for successful oocyte development to the preimplantation embryos. PMID:19712552

  13. CHARACTERIZATION OF THE YOUNG OPEN CLUSTER G144.9+0.4 IN THE CAMELOPARDALIS OB1 ASSOCIATION

    SciTech Connect

    Lin, Chien-Cheng; Chen, W. P.; Panwar, Neelam

    2013-10-01

    Our star-count analysis of the Two Micron All Sky Survey point sources resulted in an identification of the star cluster G144.9+0.4. The cluster was found, but not characterized, by Glushkova et al. We show that the cluster is physically associated with the Cam OB1 association at a distance of about 1 kpc and with an age of 1-2 Myr. Pre-main sequence stars are identified on the basis of photometric and proper motion data. A total of 91 additional OB star candidates was found in subgroups 1A and 1B, a significant increase from the currently known 43 OB stars. The OB members show an age spread that indicates a sustained star formation for at least the last 10-15 Myr. The young cluster G144.9+0.4 represents the latest episode of sequential star formation in this cloud complex.

  14. Candidate X-ray-emitting OB Stars in the Carina Nebula Identified Via Infrared Spectral Energy Distributions

    NASA Astrophysics Data System (ADS)

    Povich, Matthew S.; Townsley, Leisa K.; Broos, Patrick S.; Gagné, Marc; Babler, Brian L.; Indebetouw, Rémy; Majewski, Steven R.; Meade, Marilyn R.; Getman, Konstantin V.; Robitaille, Thomas P.; Townsend, Richard H. D.

    2011-05-01

    We report the results of a new survey of massive, OB stars throughout the Carina Nebula using the X-ray point source catalog provided by the Chandra Carina Complex Project (CCCP) in conjunction with infrared (IR) photometry from the Two Micron All-Sky Survey and the Spitzer Space Telescope Vela-Carina survey. Mid-IR photometry is relatively unaffected by extinction, hence it provides strong constraints on the luminosities of OB stars, assuming that their association with the Carina Nebula, and hence their distance, is confirmed. We fit model stellar atmospheres to the optical (UBV) and IR spectral energy distributions (SEDs) of 182 OB stars with known spectral types and measure the bolometric luminosity and extinction for each star. We find that the extinction law measured toward the OB stars has two components: AV = 1-1.5 mag produced by foreground dust with a ratio of total-to-selective absorption RV = 3.1 plus a contribution from local dust with RV > 4.0 in the Carina molecular clouds that increases as AV increases. Using X-ray emission as a strong indicator of association with Carina, we identify 94 candidate OB stars with L bol >~ 104 L sun by fitting their IR SEDs. If the candidate OB stars are eventually confirmed by follow-up spectroscopic observations, the number of cataloged OB stars in the Carina Nebula will increase by ~50%. Correcting for incompleteness due to OB stars falling below the L bol cutoff or the CCCP detection limit, these results potentially double the size of the young massive stellar population.

  15. A M2FS Spectroscopic Study of Low-mass Young Stars in Orion OB1

    NASA Astrophysics Data System (ADS)

    Kaleida, Catherine C.; Briceno, Cesar; Calvet, Nuria; Mateo, Mario L.; Hernandez, Jesus

    2015-01-01

    Surveys of pre-main sequence stars in the ~4-10 Myr range provide a window into the decline of the accretion phase of stars and the formation of planets. Nearby star clusters and stellar associations allow for the study of these young stellar populations all the way down to the lowest mass members. One of the best examples of nearby 4-10 Myr old stellar populations is the Orion OB1 association. The CIDA Variability Survey of Orion OB1 (CVSO - Briceño et al. 2001) has used the variability properties of low-mass pre-main-sequence (PMS) stars to identify hundreds of K and M-type stellar members of the Orion OB1 association, a number of them displaying IR-excess emission and thought to be representative of more evolved disk-bearing young stars. Characterizing these young, low-mass objects using spectroscopy is integral to understanding the accretion phase in young stars. We present preliminary results of a spectroscopic survey of candidate and confirmed Orion OB1 low-mass members taken during November 2014 and February 2014 using the Michigan/Magellan Fiber Spectrograph (M2FS), a PI instrument on the Magellan Clay Telescope (PI: M. Matteo). Target fields located in the off-cloud regions of Orion were identified in the CVSO, and observed using the low and high-resolution modes of M2FS. Both low and high-resolution spectra are needed in order to confirm membership and derive masses, ages, kinematics and accretion properties. Initial analysis of these spectra reveal many new K and M-type members of the Orion OB1 association in these low extinction, off-cloud areas. These are the more evolved siblings of the youngest stars still embedded in the molecular clouds, like those in the Orion Nebula Cluster. With membership and spectroscopic indicators of accretion we are building the most comprehensive stellar census of this association, enabling us to derive a robust estimate of the fraction of young stars still accreting at a various ages, a key constraint for the end of

  16. OB stars at the lowest Local Group metallicity. GTC-OSIRIS observations of Sextans A

    NASA Astrophysics Data System (ADS)

    Camacho, I.; Garcia, M.; Herrero, A.; Simón-Díaz, S.

    2016-01-01

    Context. Massive stars play an important role in the chemical and dynamical evolution of the Universe. The first metal-poor stars may have started the reionization of the Universe. To understand these early epochs it is necessary to know the behavior and the physical properties of massive stars in very metal-poor environments. We focus on the massive stellar content of the metal-poor irregular galaxy Sextans A. Aims: Our aim is to find and classify OB stars in Sextans A, so as to later determine accurate stellar parameters of these blue massive stars in this low-metallicity region (Z ~ 0.1 Z⊙). Methods: Using UBV photometry, the reddening-free index Q and GALEX imaging, we built a list of blue massive star candidates in Sextans A. We obtained low-resolution (R ~ 1000) GTC-OSIRIS spectra for a fraction of them and carried out spectral classification. For the confirmed O-stars, we derived preliminary stellar parameters. Results: The target selection criteria and observations were successful and have produced the first spectroscopic atlas of OB-type stars in Sextans A. From the whole sample of 18 observed stars, 12 were classified as early OB-types, including 5 O-stars. The radial velocities of all target stars are in agreement with their Sextans A membership, although three of them show significant deviations. We determined the stellar parameters of the O-type stars using the stellar atmosphere code FASTWIND and revisited the sub-SMC temperature scale. Two of the O-stars are consistent with relatively strong winds and enhanced helium abundances, although results are not conclusive. We discuss the position of the OB stars in the HRD. Initial stellar masses run from slightly below 20 up to 40 solar masses. Conclusions: The target selection method worked well for Sextans A. The stellar temperatures are consistent with findings in other galaxies. Some of the targets deserve follow-up spectroscopy because of indications of a runaway nature, an enhanced helium abundance

  17. MUG-OBS - Multiparameter Geophysical Ocean Bottom System : a new instrumental approach to monitor earthquakes.

    NASA Astrophysics Data System (ADS)

    Hello, Y.; Yegikyan, M.; Charvis, P.; Verfaillie, R.; Philippe, O.

    2015-12-01

    There are several attempts to monitor real time seismic activity, using regional scale wired nodes, such as Neptune in Canada and in the U.S, Antares in France or DONET in Japan.On another hand there are also initiatives in deploying repeatedly OBS array like during the amphibious Cascadia Initiative (four 1-year deployments), the Japanese Pacific Array (broadband OBSs "ocean-bottom broadband dispersion survey" with 2-years autonomy), the Obsismer program in the French Lesser Antilles (eight 6-months deployments) and the Osisec program in Ecuador (four 6-months deployments). These OBSs are autonomous, they are self-recovered or recovered using an ROV. These systems are costly including ship time, and require to recover the OBS before to start working on data.Among the most recent alternative we developed a 3-years autonomy OBS equipped with a Nanometrics Trillium 120 s, a triaxial accelerometer, a differential, an absolute pressure gauge, and a hydrophone. MUG-OBS is a free falling instrument rated down to 6000 m. The installation of the sensor is monitored by acoustic commands from the surface and a health bulletin with data checking is recovered by acoustic during the installation. The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a seismic crisis) utilizing one of the 6 data-shuttles released from the surface by acoustic command using a one day fast cruise boat of opportunity. Since sensors stayed at the same location for 3 years (when an OBS is redeployed on the same site, it will not land in the same place), it is a perfect tool to monitor slow seismic events, background seismic activity and aftershock distribution. Clock, drift measurement and GPS localization is automatic when the shuttle reaches the surface. A new version is being developed; for remote areas, shuttles released automatically and a seismic events bulletin is transmitted. Selected data can be recovered by two- way Iridium

  18. Spatial patterns of the evolution of the chemical composition and discharge of river water in the Ob River basin

    NASA Astrophysics Data System (ADS)

    Savichev, O. G.; Mazurov, A. K.; Pipko, I. I.; Sergienko, V. I.; Semiletov, I. P.

    2016-01-01

    The discharge of major cations and dissolved organic carbon (Corg) with water of the Ob River and its tributaries along the natural zones within the Ob River basin was calculated, and the contribution of the underground component to the volumes of total discharge of the Ob River basin was estimated. It was demonstrated that the total chemical composition of river water and the geochemical discharge in the Ob River basin were consistent with the zoned hydroclimatic conditions controlling the character and duration of interaction in the water-rock system. It was established that the average ionic discharge of the Ob River increased from 6-7 × 106 t/year near Barnaul to 46-47 × 106 t/year near Salekhard; the discharge of dissolved Corg increased from 0.1 × 106 to 3.8 × 106 t/year. Multiple enrichment of underground waters of the Ob River in dissolved organic matter from the upper to the lower reaches was revealed.

  19. Integration of a permanent OBS offshore NE Iberian Peninsula to the Catalan Seismic Network

    NASA Astrophysics Data System (ADS)

    Frontera, T.; Olivera, C.; Jara, J. A.; Goula, X.; Ugalde, A.

    2009-04-01

    On August 2005 a permanent ocean bottom seismometer (OBS) and a differential pressure gauge (DPG) were installed inside the security perimeter of the Casablanca oil platform (40 km offshore Tarragona, NE Spain), within the framework of a project which has the aim of improving the knowledge of the seismicity and seismic risk in the region. This project is being carried out by the Institut Geològic de Catalunya (IGC) and the Observatori de l'Ebre, in collaboration with the Spanish oil company Repsol Investigaciones Petrolíferas. The sensors were submerged at about 400 m to the SW of the oil platform and were deposited at about 150 m in depth. Data are digitized on-site and are transmitted through a submarine cable to the platform, where they are recorded. In July 2007 via satellite signal transmission was implemented to have continuous and real time data, which allowed integrating the OBS into the Catalan Seismic Network. Since 1999 the objectives of the Catalan Seismic Network are, on the one hand, providing rapid information for Civil Defence Services and society in general and, on the other hand, to obtain systematically high quality data for the scientific community. This real time system is based on a VSAT seismic network and it has been implemented in Catalonia (Spain) by the IGC. The project of the network was planned to create robust, high performance field infrastructures through the installation of up to 21 stations equipped with three component broad band sensors and a high dynamic range and it has been developed in several steps. In 2009, 18 stations are operative: 14 broad band and 3 accelerometers on land and one broad band OBS. The stations are based on VSAT platforms that are transmitting continuously almost real time seismic data via satellite to the IGC hub. Once at seismic data reception centre data are continuously archived and processed with an automatic system. A study of the OBS signal in terms of noise has been made and compared to the

  20. Green tea extract protects against nonalcoholic steatohepatitis in ob/ob mice by decreasing oxidative and nitrative stress responses induced by proinflammatory enzymes.

    PubMed

    Chung, Min-Yu; Park, Hea Jin; Manautou, Jose E; Koo, Sung I; Bruno, Richard S

    2012-04-01

    Oxidative and nitrative stress responses resulting from inflammation exacerbate liver injury associated with nonalcoholic steatohepatitis (NASH) by inducing lipid peroxidation and protein nitration. The objective of this study was to investigate whether the anti-inflammatory properties of green tea extract (GTE) would protect against NASH by suppressing oxidative and nitrative damage mediated by proinflammatory enzymes. Obese mice (ob/ob) and their 5-week-old C57BL6 lean littermates were fed 0%, 0.5% or 1% GTE for 6 weeks (n=12-13 mice/group). In obese mice, hepatic lipid accumulation, inflammatory infiltrates and serum alanine aminotransferase activity were markedly increased, whereas these markers of hepatic steatosis, inflammation and injury were significantly reduced among obese mice fed GTE. GTE also normalized hepatic 4-hydroxynonenal and 3-nitro-tyrosine (N-Tyr) concentrations to those observed in lean controls. These oxidative and nitrative damage markers were correlated with alanine aminotransferase (P<.05; r=0.410-0.471). Improvements in oxidative and nitrative damage by GTE were also associated with lower hepatic nicotinamide adenine dinucleotide phosphate oxidase activity. Likewise, GTE reduced protein expression levels of hepatic myeloperoxidase and inducible nitric oxide synthase and decreased the concentrations of nitric oxide metabolites. Correlative relationships between nicotinamide adenine dinucleotide phosphate oxidase and hepatic 4-hydroxynonenal (r=0.364) as well as nitric oxide metabolites and N-Tyr (r=0.598) suggest that GTE mitigates lipid peroxidation and protein nitration by suppressing the generation of reactive oxygen and nitrogen species. Further study is warranted to determine whether GTE can be recommended as an effective dietary strategy to reduce the risk of obesity-triggered NASH. PMID:21543212

  1. Inhibition of Leptin-ObR Interaction Does not Prevent Leptin Translocation Across a Human Blood-Brain Barrier Model.

    PubMed

    Gonzalez-Carter, D; Goode, A E; Fiammengo, R; Dunlop, I E; Dexter, D T; Porter, A E

    2016-06-01

    The adipocyte-derived hormone leptin regulates appetite and energy homeostasis through the activation of leptin receptors (ObR) on hypothalamic neurones; hence, leptin must be transported through the blood-brain barrier (BBB) to reach its target sites in the central nervous system. During obesity, however, leptin BBB transport is decreased, in part precluding leptin as a viable clinical therapy against obesity. Although the short isoform of the ObR (ObRa) has been implicated in the transport of leptin across the BBB as a result of its elevated expression in cerebral microvessels, accumulating evidence indicates that leptin BBB transport is independent of ObRa. In the present study, we employed an ObR-neutralising antibody (9F8) to directly examine the involvement of endothelial ObR in leptin transport across an in vitro human BBB model composed of the human endothelial cell line hCMEC/D3. Our results indicate that, although leptin transport across the endothelial monolayer was nonparacellular, and energy- and endocytosis-dependent, it was not inhibited by pre-treatment with 9F8, despite the ability of the latter to recognise hCMEC/D3-expressed ObR, prevent leptin-ObR binding and inhibit leptin-induced signal transducer and activator of transcription 3 (STAT-3) phosphorylation in hCMEC/D3 cells. Furthermore, hCMEC/D3 cells expressed the transporter protein low-density lipoprotein receptor-related protein-2 (LRP-2), which is capable of binding and endocytosing leptin. In conclusion, our results demonstrate that leptin binding to and signalling through ObR is not required for efficient transport across human endothelial monolayers, indicating that ObR is not the primary leptin transporter at the human BBB, a role which may fall upon LRP-2. A deeper understanding of leptin BBB transport will help clarify the exact causes for leptin resistance seen in obesity and aid in the development of more efficient BBB-penetrating leptin analogues. PMID:27037668

  2. Ocean Basalt Simulator version 1 (OBS1): Trace element mass balance in adiabatic melting of a pyroxenite-bearing peridotite

    NASA Astrophysics Data System (ADS)

    Kimura, J. I.; Kawabata, H.

    2014-12-01

    We present a new numerical mass balance model for estimating the source conditions of a primary ocean basalt from adiabatic melting of a pyroxenite-bearing peridotite. The Ocean Basalt Simulator version 1 (OBS1) uses a thermodynamic model of adiabatic melting of a pyroxenite-bearing peridotite with experimentally parameterized liquidus-solidus and melting intervals and thermodynamically/experimentally parameterized source mineralogy of pyroxenite and peridotite. OBS1 uses a sequence of adiabatic melting with pyroxenite melting, melt metasomatism in the host peridotite, and melting of the metasomatized peridotite. OBS1 explores (1) the fractions of peridotite and pyroxenite, (2) mantle potential temperature Tp, (3) depth of termination of melting Pmt, (4) degree of melting F, and (5) residual mode Xa of the sources. The model also examines the mass balance of 26 incompatible trace elements in the sources and in the generated basalt. OBS1 can also calculate Sr-Nd-Hf-Pb isotope compositions in the sources and in the melt. OBS1 is coded in an Excel spreadsheet and runs with VBA macros. Using OBS1, we examine the source conditions of the mid oceanic ridge basalts (MORBs), plume-influenced ocean ridge basalts in Galápagos, Loihi-Loa-Koolau basalts in the Hawaiian hotspot, high-mu (HIMU) basalt from the St. Helena hotspot, and Shatsky Rise and Jurassic Mikabu oceanic plateau basalts and picrites. OBS1 is a unique model that provides an opportunity to examine the source mantle conditions using incompatible trace elements and relevant isotopes, which are key factors in global mantle recycling.

  3. VLBI Measurements of Plasma Turbulence Associated with the Cygnus OB1 Association

    NASA Astrophysics Data System (ADS)

    Spangler, Steven R.; Cordes, James M.

    1998-10-01

    We have made dual-frequency (1.67 and 5.00 GHz) VLBI observations of five compact, presumably extragalactic radio sources in the Galactic plane in the constellation of Cygnus. The lines of sight to these sources pass through a part of the interstellar medium that is modified by the Cygnus OB1 association. The VLBI observations were processed to yield measurements of the scattering measure due to interstellar plasma turbulence. The dual-frequency VLBI observations allowed estimates of the possible intrinsic structure contamination of the scattering measurements. Such an error is estimated to be less than 5% of the scattering measure for our two best-observed cases, and 15% to as high as 30% for a more weakly scattered source. Modeling the spatial power spectrum of the turbulence by Pδn(q) = C2Nq-α, where q is the spatial wavenumber of the turbulent fluctuations, our observations provide a measurement of 0LC2Ndz, where L is the thickness of the scattering medium and z is a coordinate along the line of sight. When combined with our earlier observations of the radio source 2013+370, we have a total of six lines of sight through the Cygnus OB1 association. Our observations show that the scattering through the Cygnus OB1 association is heavy and that the scattering measures vary from 0.14 to 2.21 m-20/3 kpc on lines of sight separated by as little as 1°-2°. When combined with measurements of the emission measure in the same directions, our scattering-measure results constrain properties of the turbulence in the Cygnus OB1 association. Specifically, if ε is the normalized amplitude of the density fluctuations, and l0 is the outer scale to the Kolmogorov spectrum, then our combined scattering measure-emission measure data set constrains the quantity ε2/(1+ε2)l2/30. The mean value is ~4.3 × 10-13 cm-2/3, with a range of about 0.5 in the logarithm. We do not have sufficient information to determine ε and l0 separately, but plausible ranges are ε < 1 and l0 < 3 pc

  4. OB Associations at the Upper End of the Milky Way Luminosity Function

    NASA Astrophysics Data System (ADS)

    Rahman, Mubdi; Matzner, Christopher D.; Moon, Dae-Sik

    2013-04-01

    The Milky Way's most luminous, young, and massive (M gsim 104 M ⊙) star clusters and OB associations have largely evaded detection despite knowledge of their surrounding H II regions. We search for these clusters and associations within the 40 star-forming complexes from Rahman & Murray in the 13 most luminous Wilkinson Microwave Anisotropy Probe (WMAP) free-free emission sources of the Galaxy. Selecting for objects with the dust-reddened colors of OB stars, we identify new candidate associations using the Two Micron All Sky Survey point-source catalog. In 40 star-forming complexes searched, 22 contain cluster/association candidates with sizes and masses in the range of 3'-26' and 102.3-105 M ⊙. Of the 22 candidates, at least seven have estimated masses >~ 104 M ⊙, doubling the number of such massive clusters known in the Galaxy. Only one of the searched WMAP sources remains without a candidate. Applying our method to a statistically similar set of test locations, we estimate that 3.0 ± 0.6 of our 22 candidate associations are unrelated to the star-forming complexes. In addition, the apparent extinctions of our candidate associations correlate well with the predictions from a Galactic model. These facts, along with the clear detection of a known OB association and the previous spectral verification of one cluster found by this method, validate our method. In eight of the most luminous WMAP sources, the candidate associations can account for the observed free-free flux. With our new compilation, the Galactic census of young, massive stellar associations may now be about half complete.

  5. OPTICAL PHOTOMETRIC GTC/OSIRIS OBSERVATIONS OF THE YOUNG MASSIVE ASSOCIATION CYGNUS OB2

    SciTech Connect

    Guarcello, M. G.; Wright, N. J.; Drake, J. J.; Aldcroft, T.; Kashyap, V. L.; Garcia-Alvarez, D.; Drew, J. E.

    2012-10-15

    In order to fully understand the gravitational collapse of molecular clouds, the star formation process, and the evolution of circumstellar disks, these phenomena must be studied in different Galactic environments with a range of stellar contents and positions in the Galaxy. The young massive association Cygnus OB2, in the Cygnus-X region, is a unique target to study how star formation and the evolution of circumstellar disks proceed in the presence of a large number of massive stars. We present a catalog obtained with recent optical observations in the r, i, z filters with OSIRIS, mounted on the 10.4 m Gran Telescopio CANARIAS telescope, which is the deepest optical catalog of Cyg OB2 to date. The catalog consists of 64,157 sources down to M = 0.15 M{sub Sun} at the adopted distance and age of Cyg OB2. A total of 38,300 sources have good photometry in all three bands. We combined the optical catalog with existing X-ray data of this region, in order to define the cluster locus in the optical diagrams. The cluster locus in the r - i versus i - z diagram is compatible with an extinction of the optically selected cluster members in the 2.64{sup m} < A{sub V} < 5.57{sup m} range. We derive an extinction map of the region, finding a median value of A{sub V} = 4.33{sup m} in the center of the association, decreasing toward the northwest. In the color-magnitude diagrams, the shape of the distribution of main-sequence stars is compatible with the presence of an obscuring cloud in the foreground {approx}850 {+-} 25 pc from the Sun.

  6. A Spitzer view of the giant molecular cloud Mon OB1 East/NGC 2264

    SciTech Connect

    Rapson, V. A.; Pipher, J. L.; Gutermuth, R. A.; Megeath, S. T.; Allen, T. S.; Myers, P. C.; Allen, L. E.

    2014-10-20

    We present Spitzer 3.6, 4.5, 5.8, 8.0, and 24 μm images of the Mon OB1 East giant molecular cloud, which contains the young star forming region NGC 2264, as well as more extended star formation. With Spitzer data and Two Micron All Sky Survey photometry, we identify and classify young stellar objects (YSOs) with dusty circumstellar disks and/or envelopes in Mon OB1 East by their infrared-excess emission and study their distribution with respect to cloud material. We find a correlation between the local surface density of YSOs and column density of molecular gas as traced by dust extinction that is roughly described as a power law in these quantities. NGC 2264 follows a power-law index of ∼2.7, exhibiting a large YSO surface density for a given gas column density. Outside of NGC 2264 where the surface density of YSOs is lower, the power law is shallower and the region exhibits a larger gas column density for a YSO surface density, suggesting the star formation is more recent. In order to measure the fraction of cloud members with circumstellar disks/envelopes, we estimate the number of diskless pre-main-sequence stars by statistical removal of background star detections. We find that the disk fraction of the NGC 2264 region is 45%, while the surrounding, more distributed regions show a disk fraction of 19%. This may be explained by the presence of an older, more dispersed population of stars. In total, the Spitzer observations provide evidence for heterogenous, non-coeval star formation throughout the Mon OB1 cloud.

  7. SPITZER IMAGING OF THE NEARBY RICH YOUNG CLUSTER, Cep OB3b

    SciTech Connect

    Allen, Thomas S.; Kryukova, Erin; Thomas Megeath, S.; Gutermuth, Robert A.; Pipher, Judith L.; Naylor, Tim; Jeffries, R. D.; Wolk, Scott J.; Spitzbart, Brad; Muzerolle, James

    2012-05-10

    We map the full extent of a rich massive young cluster in the Cep OB3b association with the Infrared Array Camera and Multi-band Imaging Photometer System instruments aboard the Spitzer Space Telescope and the ACIS instrument aboard the Chandra X-Ray Observatory. At 700 pc, it is revealed to be the second nearest large (>1000 member), young (<5 Myr) cluster known. In contrast to the nearest large cluster, the Orion Nebula Cluster, Cep OB3b is only lightly obscured and is mostly located in a large cavity carved out of the surrounding molecular cloud. Our infrared and X-ray data sets, as well as visible photometry from the literature, are used to take a census of the young stars in Cep OB3b. We find that the young stars within the cluster are concentrated in two sub-clusters; an eastern sub-cluster, near the Cep B molecular clump, and a western sub-cluster, near the Cep F molecular clump. Using our census of young stars, we examine the fraction of young stars with infrared excesses indicative of circumstellar disks. We create a map of the disk fraction throughout the cluster and find that it is spatially variable. Due to these spatial variations, the two sub-clusters exhibit substantially different average disk fractions from each other: 32% {+-} 4% and 50% {+-} 6%. We discuss whether the discrepant disk fractions are due to the photodestruction of disks by the high mass members of the cluster or whether they result from differences in the ages of the sub-clusters. We conclude that the discrepant disk fractions are most likely due to differences in the ages.

  8. The discovery of low-mass pre-main-sequence stars in Cepheus OB3b

    NASA Astrophysics Data System (ADS)

    Pozzo, M.; Naylor, T.; Jeffries, R. D.; Drew, J. E.

    2003-05-01

    We report the discovery of a low-mass pre-main-sequence (PMS) stellar population in the younger subgroup of the Cepheus OB3 association, Cep OB3b, using UBVI CCD photometry and follow-up spectroscopy. The optical survey covers approximately 1300 arcmin2 on the sky and gives a global photometric and astrometric catalogue for more than 7000 objects. The location of a PMS population is well defined in a V versus (V-I) colour-magnitude diagram. Multifibre spectroscopic results for optically selected PMS candidates confirm the T Tauri nature for 10 objects, with equal numbers of classical TTS (CTTS) and weak-line TTS (WTTS). There are six other objects that we classify as possible PMS stars. The newly discovered TTS stars have masses in the range ~0.9-3.0 Msolar and ages from <1 to nearly 10 Myr, based on the Siess, Dufour & Forestini isochrones. Their location close to the O and B stars of the association (especially the O7n star) demonstrates that low-mass star formation is indeed possible in such an apparently hostile environment dominated by early-type stars and that the latter must have been less effective in eroding the circumstellar discs of their lower-mass siblings compared with other OB associations (e.g. λ-Ori). We attribute this to the nature of the local environment, speculating that the bulk of molecular material, which shielded low-mass stars from the ionizing radiation of their early-type siblings, has only recently been removed.

  9. Relevance of the expression “obs stable” in nursing observations: retrospective study

    PubMed Central

    Vijayan, Roshan; Male, Pandora

    2011-01-01

    Objective To ascertain whether use of the term “obs stable” with respect to the nursing observations is so liberal as to render it meaningless. Design Retrospective study. Setting Three teaching hospitals in London, United Kingdom. Methods We searched progress notes for the current admission of 46 inpatients for entries containing the phrases “obs stable” and “observations stable,” and reviewed the nursing observations recorded during the 24 hour period preceding each entry containing at least one phrase. We calculated the frequency of abnormalities and of persistent abnormalities (defined as occurring in every observation) observed during these 24 hour periods, and the range of observation values over a 24 hour period if at least two observations had been recorded. Results We found at least one entry in 36 (78%) progress notes (95% confidence interval 66% to 90%). Observations in the 24 hours preceding an entry included at least one abnormality for 113 (71%) of 159 cases and at least one persistent abnormality for 31 (19%). The most frequently occurring abnormalities were tachypnoea (respiratory rate ≥20 breaths/min) and hypotension (systolic blood pressure <100 mm Hg). An abnormality occurred in the observations immediately preceding an entry in 42% of cases. Mean ranges of observations over 24 hours were within the limits of normal diurnal variation, although we found that some instances of greater than normal variability were described as “stable.” Conclusions The expression “obs stable” does not reliably indicate normal observations or variations in observations within physiological limits. Doctors should avoid using the expression altogether or clarify it with further information. PMID:22187323

  10. OB ASSOCIATIONS AT THE UPPER END OF THE MILKY WAY LUMINOSITY FUNCTION

    SciTech Connect

    Rahman, Mubdi; Matzner, Christopher D.; Moon, Dae-Sik

    2013-04-01

    The Milky Way's most luminous, young, and massive (M {approx}> 10{sup 4} M{sub Sun }) star clusters and OB associations have largely evaded detection despite knowledge of their surrounding H II regions. We search for these clusters and associations within the 40 star-forming complexes from Rahman and Murray in the 13 most luminous Wilkinson Microwave Anisotropy Probe (WMAP) free-free emission sources of the Galaxy. Selecting for objects with the dust-reddened colors of OB stars, we identify new candidate associations using the Two Micron All Sky Survey point-source catalog. In 40 star-forming complexes searched, 22 contain cluster/association candidates with sizes and masses in the range of 3'-26' and 10{sup 2.3}-10{sup 5} M{sub Sun }. Of the 22 candidates, at least seven have estimated masses {approx}> 10{sup 4} M{sub Sun }, doubling the number of such massive clusters known in the Galaxy. Only one of the searched WMAP sources remains without a candidate. Applying our method to a statistically similar set of test locations, we estimate that 3.0 {+-} 0.6 of our 22 candidate associations are unrelated to the star-forming complexes. In addition, the apparent extinctions of our candidate associations correlate well with the predictions from a Galactic model. These facts, along with the clear detection of a known OB association and the previous spectral verification of one cluster found by this method, validate our method. In eight of the most luminous WMAP sources, the candidate associations can account for the observed free-free flux. With our new compilation, the Galactic census of young, massive stellar associations may now be about half complete.

  11. Assimilation of wide swath altimetry to improve modelling of the Ob river, in western Siberia

    NASA Astrophysics Data System (ADS)

    Biancamaria, Sylvain; Durand, Michael; Andreadis, Kostas; Bates, Paul; Boone, Aaron; Mognard, Nelly; Rodriguez, Ernesto; Alsdorf, Doug; Lettenmaier, Dennis

    2010-05-01

    Variations in the hydrologic cycle of Arctic rivers, due to climate change, have far-reaching consequences on the global water and carbon cycles, as well as the thermohaline circulation in the ocean. Thus, being able to monitor and accurately model Arctic rivers is a crucial issue for the understanding of changes in the Arctic water cycle. The Ob River is one of the largest rivers in the Arctic, contributing nearly 15% of total freshwater flow into the Arctic Ocean. A model of the lower Ob River has been implemented, by coupling the land surface scheme ISBA (Interactions between Soil, Biosphere and Atmosphere), developed by the CNRM (Centre National de Recherche Meteorologique, France), with the flood inundation model LISFLOOD-FP, developed by the University of Bristol, UK. The scarcity of in situ data in the Ob River basin leads to large errors in the modelling. The forecast error has been assumed to be only due to uncertainties on forcing data (precipitation and air temperature). These errors can be reduced by implementing an Ensemble Kalman smoother for assimilating remotely sensed water levels from satellite altimeters. Data from the future Surface Water and Ocean Topography (SWOT) satellite wide swath altimeter mission will be particularly well adapted to improve the modelling of the Arctic rivers. Every few days, the Ka Radar Interferometer (KaRIN) on board SWOT will provide, for the first time, a global estimate of surface water elevation (with a vertical accuracy of a few cm). The SWOT data have been simulated and assimilated to estimate and quantify their potential to reduce the modelling errors. Preliminary results, using a local Ensemble Kalman Smoother over a 3 day time frame, show that assimilation of SWOT measurements (for a 22-day repeat orbit) leads to a decrease of model prediction errors by a factor of 2.4.

  12. Winds of low-metallicity OB-type stars: HST-COS spectroscopy in IC 1613

    SciTech Connect

    Garcia, Miriam; Najarro, Francisco; Herrero, Artemio; Urbaneja, Miguel Alejandro

    2014-06-10

    We present the first quantitative ultraviolet spectroscopic analysis of resolved OB stars in IC 1613. Because of its alleged very low metallicity (≲1/10 Z {sub ☉}, from H II regions), studies in this Local Group dwarf galaxy could become a significant step forward from the Small Magellanic Cloud (SMC) toward the extremely metal-poor massive stars of the early universe. We present HST-COS data covering the ∼1150-1800 Å wavelength range with resolution R ∼ 2500. We find that the targets do exhibit wind features, and these are similar in strength to SMC stars. Wind terminal velocities were derived from the observed P Cygni profiles with the Sobolev plus Exact Integration method. The v {sub ∞}-Z relationship has been revisited. The terminal velocity of IC 1613 O stars is clearly lower than Milky Way counterparts, but there is no clear difference between IC 1613 and SMC or LMC analog stars. We find no clear segregation with host galaxy in the terminal velocities of B-supergiants, nor in the v {sub ∞}/v {sub esc} ratio of the whole OB star sample in any of the studied galaxies. Finally, we present the first evidence that the Fe-abundance of IC 1613 OB stars is similar to the SMC, which is in agreement with previous results on red supergiants. With the confirmed ∼1/10 solar oxygen abundances of B-supergiants, our results indicate that IC 1613's α/Fe ratio is sub-solar.

  13. MUG-OBS - Multiparameter Geophysical Ocean Bottom System : a new instrumental approach to monitor earthquakes.

    NASA Astrophysics Data System (ADS)

    hello, yann; Charvis, Philippe; Yegikyan, Manuk; verfaillie, Romain; Rivet, Diane

    2016-04-01

    Real time monitoring of seismic activity is a major issue for early warning of earthquakes and tsunamis. It can be done using regional scale wired nodes, such as Neptune in Canada and in the U.S, or DONET in Japan. Another approach to monitor seismic activity at sea is to deploying repeatedly OBS array like during the amphibious Cascadia Initiative (four time 1-year deployments), the Japanese Pacific Array (broadband OBSs "ocean-bottom broadband dispersion survey" with 2-years autonomy), the Obsismer program in the French Lesser Antilles (eight time 6-months deployments) and the Osisec program in Ecuador (four time 6-months deployments). These autonomous OBSs are self-recovered or recovered using an ROV. These systems are costly including ship time, and require to recover the OBS before to start working on data. Among the most recent alternative we developed a 3/4 years autonomy ocean bottom system with 9 channels (?) allowing the acquisition of different seismic or environmental parameters. MUG-OBS is a free falling instrument rated down to 6000 m. The installation of the sensor is monitored by acoustic commands from the surface and a health bulletin with data checking is recovered by acoustic during the installation. The major innovation is that it is possible to recover the data any time on demand (regularly every 6-months or after a crisis) using one of the 6 data-shuttles released from the surface by acoustic command using a one day fast cruise boat of opportunity. Since sensors stayed at the same location for 3 years, it is a perfect tool to monitor large seismic events, background seismic activity and aftershock distribution. Clock, drift measurement and GPS localization is automatic when the shuttle reaches the surface. For remote areas, shuttles released automatically and a seismic events bulletin is transmitted. Selected data can be recovered by two-way Iridium satellite communication. After a period of 3 years the main station is self-recovered by

  14. ObsPy: A Python toolbox for seismology - Current state, applications, and ecosystem around it

    NASA Astrophysics Data System (ADS)

    Krischer, L.; Megies, T.; Sales de Andrade, E.; Barsch, R.; Beyreuther, M.

    2015-12-01

    ObsPy (http://www.obspy.org) is a community-driven, open-source project offering a bridge for seismology into the scientific Python ecosystem. It provides read and write support for essentially all commonly used waveform, station, and event metadata formats with a unified interface, a comprehensive signal processing toolbox tuned to the needs of seismologists, integrated access to all large data centers, web services and databases, and convenient wrappers to third party codes like libmseed and evalresp. Python, in contrast to many other languages and tools, is simple enough to enable an exploratory and interactive coding style desired by many scientists. At the same time it is a full-fledged programming language usable by software engineers to build complex and large programs. This combination makes it very suitable for use in seismology where research code often has to be translated to stable and production ready environments. It furthermore offers many freely available high quality scientific modules covering most needs in developing scientific software.ObsPy has been in constant development for more than 5 years and nowadays enjoys a large rate of adoption in the community with thousands of users. Successful applications include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. Additionally it sparked the development of several more specialized packages slowly building a modern seismological ecosystem around it.This contribution will give a short introduction and overview of ObsPy and highlight a number of us cases and software built around it. We will furthermore discuss the issue of sustainability of scientific software.

  15. ObsPy: A Python toolbox for seismology - Current state, applications, and ecosystem around it

    NASA Astrophysics Data System (ADS)

    Lecocq, Thomas; Megies, Tobias; Krischer, Lion; Sales de Andrade, Elliott; Barsch, Robert; Beyreuther, Moritz

    2016-04-01

    ObsPy (http://www.obspy.org) is a community-driven, open-source project offering a bridge for seismology into the scientific Python ecosystem. It provides * read and write support for essentially all commonly used waveform, station, and event metadata formats with a unified interface, * a comprehensive signal processing toolbox tuned to the needs of seismologists, * integrated access to all large data centers, web services and databases, and * convenient wrappers to third party codes like libmseed and evalresp. Python, in contrast to many other languages and tools, is simple enough to enable an exploratory and interactive coding style desired by many scientists. At the same time it is a full-fledged programming language usable by software engineers to build complex and large programs. This combination makes it very suitable for use in seismology where research code often has to be translated to stable and production ready environments. It furthermore offers many freely available high quality scientific modules covering most needs in developing scientific software. ObsPy has been in constant development for more than 5 years and nowadays enjoys a large rate of adoption in the community with thousands of users. Successful applications include time-dependent and rotational seismology, big data processing, event relocations, and synthetic studies about attenuation kernels and full-waveform inversions to name a few examples. Additionally it sparked the development of several more specialized packages slowly building a modern seismological ecosystem around it. This contribution will give a short introduction and overview of ObsPy and highlight a number of use cases and software built around it. We will furthermore discuss the issue of sustainability of scientific software.

  16. Nature vs. Nurture: The influence of OB star environments on proto-planetary disk evolution.

    NASA Astrophysics Data System (ADS)

    Bouwman, Jeroen; Feigelson, Eric; Getman, Kostantin; Henning, Thomas; Lawson, Warrick; Linz, Hendrik; Luhman, Kevin; Roccatagliata, Veronica; Sicilia Aguilar, Aurora; Townsley, Leisa; Wang, Junfeng

    2006-05-01

    A natural approach for understanding the origin and diversity of planetary systems is to study the birth sites of planetary systems under varying environmental conditions. Dust grains in protoplanetary disks, the building blocks of planets, are structurally and chemically altered, and grow through coagulation into planetesimals. The disk geometry may change from a flaring to a more flattened structure, gaps may develop under the gravitational influence of protoplanets, and eventually the disk will dissipate, terminating the planet formation process. While the infrared properties of disks in quiet cloud environments have been extensively studied, investigations under the conditions of strong UV radiation and stellar winds in the proximity of OB stars have been limited. We propose a combined IRAC/IRS study of a large, well-defined and unbiased X-ray selected sample of pre-main-sequence stars in three OB associations: Pismis 24 in NGC 6357, NGC 2244 in the Rosette Nebula, and IC 1795 in the W3 complex. The samples are based on recent Chandra X-ray Observatory studies which reliably identify hundreds of cluster members and were carefully chosen to avoid high infrared nebular background. A new Chandra exposure of IC 1795 is requested, and an optical followup to characterise the host stars is planned. Modelling the Spitzer findings will provide the composition and size of dust present as well as the geometry, mass, and gaps in the global structure of the disk. As hundreds of cluster members will be covered with IRAC and dozens with IRS, good statistics on the disk evolution and dispersal as a function of location with respect to OB stars will be obtained. Comparison of disk properties within our sample and with existing Spitzer studies of quiescent star-forming regions should significantly advance the aim of characterising the influence of the environment on the evolution of protoplanetary disks. This effort relies on a powerful synergy between the Chandra and Spitzer

  17. New polarization measurements of HD 183143, HD 204827, and CYG OB 2 Sch. No. 12

    NASA Astrophysics Data System (ADS)

    Schulz, A.; Lenzen, R.

    1983-05-01

    We have carried out measurements of linear polarization of HD 183143, HD 204827, and Cyg OB2 Schulte No. 12 in the spectral range of 0.3-1.1 μm obtaining accurate data which might be used in the future for instrumental calibrations. Combining them with recently published near infrared polarization data for these 3 stars we find an excellent agreement with the wavelength dependence of interstellar polarization given by Wilking et al. (1980) using a slightly changed width of the fit curve.

  18. BOREAS TF-8 NSA-OJP and SSA-OBS Ceilometer Data

    NASA Technical Reports Server (NTRS)

    Moore, Kathleen E.; Hall, Forrest G. (Editor); Huemmrich, Karl (Editor); Fitzjarrald, David R.

    2000-01-01

    The BOREAS TF-8 team used ceilometers to collect data on the fraction of the sky covered with clouds and the cloud height. Included with these data is the surface-based lifting condensation level, derived from temperature and humidity values acquired at the flux tower at the NSA-OJP site. Ceilo-meter data were collected at the NSA-OJP site in 1994 and at the NSA-OJP and SSA-OBS sites in 1996. The data are available in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884).

  19. Methane and Trichloroethylene Degradation by Methylosinus trichosporium OB3b Expressing Particulate Methane Monooxygenase

    PubMed Central

    Lontoh, Sonny; Semrau, Jeremy D.

    1998-01-01

    Whole-cell assays of methane and trichloroethylene (TCE) consumption have been performed on Methylosinus trichosporium OB3b expressing particulate methane monooxygenase (pMMO). From these assays it is apparent that varying the growth concentration of copper causes a change in the kinetics of methane and TCE degradation. For M. trichosporium OB3b, increasing the copper growth concentration from 2.5 to 20 μM caused the maximal degradation rate of methane (Vmax) to decrease from 300 to 82 nmol of methane/min/mg of protein. The methane concentration at half the maximal degradation rate (Ks) also decreased from 62 to 8.3 μM. The pseudo-first-order rate constant for methane, Vmax/Ks, doubled from 4.9 × 10−3 to 9.9 × 10−3 liters/min/mg of protein, however, as the growth concentration of copper increased from 2.5 to 20 μM. TCE degradation by M. trichosporium OB3b was also examined with varying copper and formate concentrations. M. trichosporium OB3b grown with 2.5 μM copper was unable to degrade TCE in both the absence and presence of an exogenous source of reducing equivalents in the form of formate. Cells grown with 20 μM copper, however, were able to degrade TCE regardless of whether formate was provided. Without formate the Vmax for TCE was 2.5 nmol/min/mg of protein, while providing formate increased the Vmax to 4.1 nmol/min/mg of protein. The affinity for TCE also increased with increasing copper, as seen by a change in Ks from 36 to 7.9 μM. Vmax/Ks for TCE degradation by pMMO also increased from 6.9 × 10−5 to 5.2 × 10−4 liters/min/mg of protein with the addition of formate. From these whole-cell studies it is apparent that the amount of copper available is critical in determining the oxidation of substrates in methanotrophs that are expressing only pMMO. PMID:16349516

  20. Luminosity functions and color-magnitude diagrams for three OB associations in the Large Magellanic Cloud

    NASA Technical Reports Server (NTRS)

    Degioia-Eastwood, K.; Meyers, R. P.; Jones, D. P.

    1993-01-01

    Using the point spread function photometry program DAOPHOT, we have used UBV CCD photometry to construct color-magnitude diagrams and luminosity functions for three OB associations in the Large Magellanic Cloud. The region LH 76 appears to be completely coeval; the region LH 13 shows some evidence for noncoevality which will need to be checked with spectra of the stars in question. The region LH 105, which lies on the southern edge of 30 Doradus, shows significant contamination by an underlying older population, possibly from previous star forming events. The luminosity functions, which serve as the first step toward determining the initial mass function in these regions, are calculated.

  1. New Low-mass Accretors in the Scorpius-Centaurus OB Association

    NASA Astrophysics Data System (ADS)

    Murphy, Simon J.; Lawson, Warrick A.; Bento, Joao

    2016-01-01

    We describe the serendipitous discovery of two new lithium-rich M5 members of the Scorpius-Centaurus OB Association (Sco-Cen). Both stars exhibit large 12 and 22 μm excesses and strong, variable Hα emission which we attribute to accretion from circumstellar discs. Such stars are thought to be incredibly rare at the ~16 Myr median age of much of Sco-Cen. The serendipitous discovery of two accreting stars hosting large quantities of circumstellar material may be indicative of a sizeable age spread in Sco-Cen, or further evidence that disc dispersal and planet formation time-scales are longer around lower-mass stars.

  2. An RWA algorithm for OBS networks based on iterative local optimization of total blocking probability

    NASA Astrophysics Data System (ADS)

    Yoshikawa, Tomohiro; Nagashima, Hidetaka; Hasegawa, Hiroshi; Sato, Ken-ichi

    2007-11-01

    We propose a routing and wavelength assignment algorithm for Optical Burst Switching (OBS) networks that utilizes centralized control. First, a method that can estimate the expected total blocking time in the network is presented. Then the proposed algorithm minimizes the estimated blocking time by simple iterative local optimization in terms of the traffic demand between each pair of nodes. We demonstrate that the proposed algorithm attains much smaller blocking probability than conventional distributed control algorithms. It is also shown that with introduction of optical buffers and burst retransmission, the proposed method realizes low burst loss rates (<10 -6) acceptable for most applications.

  3. Nature vs. Nurture: The influence of OB star environments on proto-planetary disk evolution

    NASA Astrophysics Data System (ADS)

    Bouwman, Jeroen

    2006-09-01

    We propose a combined IRAC/IRS study of a large, well-defined and unbiased X-ray selected sample of pre-main-sequence stars in three OB associations: Pismis 24 in NGC 6357, NGC 2244 in the Rosette Nebula, and IC 1795 in the W3 complex. The samples are based on recent Chandra X-ray Observatory studies which reliably identify hundreds of cluster members and were carefully chosen to avoid high infrared nebular background. A new Chandra exposure of IC 1795 is requested, and an optical followup to characterise the host stars is planned.

  4. A CANDIDATE FOR THE MOST LUMINOUS OB ASSOCIATION IN THE GALAXY

    SciTech Connect

    Rahman, Mubdi; Matzner, Christopher; Moon, Dae-Sik

    2011-02-20

    The Milky Way harbors giant H II regions, which may be powered by star complexes more luminous than any known Galactic OB association. Being across the disk of the Galaxy, however, these brightest associations are severely extinguished and confused. We present a search for one such association toward the most luminous H II region in the recent catalog by Murray and Rahman, which, at {approx}9.7 kpc, has a recombination rate of {approx}7 x 10{sup 51} s{sup -1}. Prior searches have identified only small-scale clustering around the rim of this shell-like region, but the primary association has not previously been identified. We apply a near-infrared color selection and find an overdensity of point sources toward its southern central part. The colors and magnitudes of these excess sources are consistent with O- and early B-type stars at extinctions 0.96 < A{sub K} < 1.2, and they are sufficiently numerous (406 {+-} 102 after subtraction of field sources) to ionize the surrounding H II region, making this a candidate for the most luminous OB association in the Galaxy. We reject an alternate theory, in which the apparent excess is caused by localized extinction, as inconsistent with source demographics.

  5. Trichloroethylene degradation using recombinant bacteria expressing the soluble methane monooxygenase from methylosinus trichosporium OB3b

    SciTech Connect

    Jahng, D.; Kim, C.; Wood, T.K.

    1995-12-01

    Soluble methane monooxygenase (sMMO) from M. trichosporium OB3b has the ability to degrade many halogenated aliphatic compounds that are found in contaminated soil and groundwater. For efficient trichloroethylene (TCE) degradation in a foreign host, efforts are being made to improve inconsistent and low sMMO activity of the recombinant strain constructed previously (Pseudomonas putida F1/pSMMO20). Additional smmo-containing recombinant strains have been constructed including various Pseudomonas, Agrobacterium, and Rhizobium strains. Recombinant facultative methylotrophs containing the smmo locus were also constructed through electroporation and tri-parental mating using a new plasmid pSMMO50. TCE degradation by these recombinant strains was examined. The effect of metal ions on in vitro sMMO activity was also discerned to optimize the expression medium. Among the metal ions examined, Cu(I), Cu(II), Ni(II), and Zn(II) inhibited sMMO purified from trichosporium OB3b, and the effect of the metal ions on each of the components of sMMO will also be discussed. In addition, the post-segregational killing locus (hok/sok) from E. coli plasmid R1 was inserted downstream of the smmo locus to stabilize the recombinant plasmid in these host cells, and chemostat cultures were used to optimize expression of active sMMO by varying the growth rate.

  6. A Candidate for the Most Luminous OB Association in the Galaxy

    NASA Astrophysics Data System (ADS)

    Rahman, Mubdi; Matzner, Christopher; Moon, Dae-Sik

    2011-02-01

    The Milky Way harbors giant H II regions, which may be powered by star complexes more luminous than any known Galactic OB association. Being across the disk of the Galaxy, however, these brightest associations are severely extinguished and confused. We present a search for one such association toward the most luminous H II region in the recent catalog by Murray & Rahman, which, at ~9.7 kpc, has a recombination rate of ~7 × 1051 s-1. Prior searches have identified only small-scale clustering around the rim of this shell-like region, but the primary association has not previously been identified. We apply a near-infrared color selection and find an overdensity of point sources toward its southern central part. The colors and magnitudes of these excess sources are consistent with O- and early B-type stars at extinctions 0.96 < AK < 1.2, and they are sufficiently numerous (406 ± 102 after subtraction of field sources) to ionize the surrounding H II region, making this a candidate for the most luminous OB association in the Galaxy. We reject an alternate theory, in which the apparent excess is caused by localized extinction, as inconsistent with source demographics.

  7. La binaria LSS 3074 y su entorno: ?`una nueva asociación OB?

    NASA Astrophysics Data System (ADS)

    Niemela, V.; Morrell, N.; Corti, M.

    En este trabajo presentamos un nuevo análisis orbital de LSS~3074, junto con tipos espectrales y velocidades radiales de estrellas que podrían constituir con ella una nueva asociación OB. La estrella O4f LSS3074 fue descubierta como binaria espectroscópica de corto período y líneas dobles por Morrell & Niemela (1990, ASP Conf. Ser. 7, 57). Posteriormente, Haefner et~al.(1994, IBVS 3969) encontraron variaciones fotométricas, estimando una inclinación orbital entre 50o y 55o. Teniendo en cuenta la importancia de obtener valores empíricos para las masas de estrellas O tempranas, y considerando la gran dispersión existente entre los valores observados y su discrepancia con los predichos por los modelos teóricos, hemos obtenido nuevas observaciones espectroscópicas de este sistema, con el propósito de mejorar los elementos orbitales derivados en la solución preliminar. Además, como las estrellas O tempranas suelen formar parte de cúmulos y asociaciones OB, hemos llevado a cabo una investigación espectroscópica de varias estrellas tempranas que podrían estar físicamente relacionadas con LSS~3074.

  8. Investigation of Cyg OB2 #11(O5 Ifc) by Modelling its Atmosphere

    NASA Astrophysics Data System (ADS)

    Maryeva, Olga; Zhuchkov, Roman; Malogolovets, Eugene

    2014-04-01

    We continue the study of O-supergiants belonging to the association Cyg OB2 using moderate-resolution spectra. In this paper we present results of the modelling of the stellar atmosphere of Cyg OB2 #11. This object belongs to the spectral class Ofc, which was recently introduced and is yet small in numbers. Ofc class consists of stars with normal spectra with CIII λλ4647, 4650, 4652 emission lines of comparable intensity to those of the Of-defining lines NIII λλ4634, 4640, 4642. We combined new spectral data obtained by the 1.5-m Russian-Turkish telescope with spectra from MAST and CASU archives and determined physical parameters of the wind and chemical composition of the stellar atmosphere using cmfgen code. The estimated nitrogen abundance is lower than one in atmospheres of `normal' O-supergiants (i.e. O4-6 supergiants without additional spectral index `n' or `c') and carbon abundance is solar. Also we find an excess in silicon. We present an illustrative comparison of our modelling results with current Geneva evolutionary models for rotating massive stars. The position on the Hertzsprung-Russell diagram corresponds to the star mass of about 50 M⊙ and age about 4.5 Myr. Moreover, we carried out the high angular resolution (~ 0.02arcsec) observations on the Russian 6-m telescope aiming to find weaker companions of this star, which did not reveal any.

  9. Analysis of interstellar extinction towards the hypergiant Cyg OB2 No. 12

    NASA Astrophysics Data System (ADS)

    Maryeva, O. V.; Chentsov, E. L.; Goranskij, V. P.; Karpov, S. V.

    The Cyg OB2 stellar association hosts an entire zoo of unique objects, and among them -- an enigmatic star Cyg OB2 No. 12 (Schulte 12, MT 304). MT 304 is enigmatic not only due to its highest luminosity (according to various estimates, it is one of the brightest stars in the Galaxy), but also because its reddening is anomalously large, greater than the mean reddening in the association. To explain the nature of anomalous reddening (AV≃ 10 mag) of MT 304, we carried out spectrophotometric observations of 25 stars located in its vicinity. We mapped interstellar extinction within the 2.5 arcmin radius and found it to increase towards MT 304. According to our results, the two most reddened stars in the association after MT 304 are J203240.35+411420.1 and J203239.90+411436.2, both located about 15 arcsec from it. Interstellar extinction AV towards these stars is about 9 mag. Our results favor the hypothesis of the circumstellar nature of reddening excess. In the second part of the paper we present the results of our modeling of MT 282 (B1 IV) and MT 343 (B1 V), which belong to the older population of the association and have ages greater than 10 Myr.

  10. A model for the infrared emission from an OB star cluster environment

    NASA Technical Reports Server (NTRS)

    Leisawitz, D.

    1991-01-01

    A model for the infrared emission from the neighborhood of an OB star cluster is described. The distribution of gas and dust around the stars, properties of the dust, and the cluster and interstellar radiation fields are variable. The model can be applied to regions around clusters embedded to various degrees in their parental molecular clouds (i.e., compact H II regions, blister-type H II regions, and the tenuous H II regions ionized by naked O stars). The model is used to simulate IRAS observations of a typical blister H II region. Infrared surface brightness and spectral energy distributions are predicted and the impact of limited spatial resolution is illustrated. The model results are shown to be consistent with observations of the exemplary outer Galaxy OB cluster NGC 7380. It is planned to use the model as a diagnostic tool to probe the physical conditions and dust properties in star-formation regions and, ultimately, in an interpretation of the spectral energy distributions of spiral galaxies.

  11. An Adaptive Loss-Aware Flow Control Scheme for Delay-Sensitive Applications in OBS Networks

    NASA Astrophysics Data System (ADS)

    Jeong, Hongkyu; Choi, Jungyul; Mo, Jeonghoon; Kang, Minho

    Optical Burst Switching (OBS) is one of the most promising switching technologies for next generation optical networks. As delay-sensitive applications such as Voice-over-IP (VoIP) have recently become popular, OBS networks should guarantee stringent Quality of Service (QoS) requirements for such applications. Thus, this paper proposes an Adaptive Loss-aware Flow Control (ALFC) scheme, which adaptively decides on the burst offset time based on loss-rate information delivered from core nodes for assigning a high priority to delay-sensitive application traffic. The proposed ALFC scheme also controls the upper-bounds of the factors inducing delay and jitter for guaranteeing the delay and jitter requirements of delay-sensitive application traffic. Moreover, a piggybacking method used in the proposed scheme accelerates the guarantee of the loss, delay, and jitter requirements because the response time for flow control can be extremely reduced up to a quarter of the Round Trip Time (RTT) on average while minimizing the signaling overhead. Simulation results show that our mechanism can guarantee a 10-3 loss-rate under any traffic load while offering satisfactory levels of delay and jitter for delay-sensitive applications.

  12. Accretion and outflow in the proplyd-like objects near Cygnus OB2

    SciTech Connect

    Guarcello, M. G.; Drake, J. J.; Wright, N. J.; García-Alvarez, D.; Kraemer, K. E.

    2014-09-20

    Cygnus OB2 is the most massive association within 2 kpc from the Sun, hosting hundreds of massive stars, thousands of young low mass members, and some sights of active star formation in the surrounding cloud. Recently, 10 photoevaporating proplyd-like objects with tadpole-shaped morphology were discovered in the outskirts of the OB association, approximately 6-14 pc away from its center. The classification of these objects is ambiguous, being either evaporating residuals of the parental cloud that are hosting a protostar inside or disk-bearing stars with an evaporating disk, such as the evaporating proplyds observed in the Trapezium Cluster in Orion. In this paper, we present a study based on low-resolution optical spectroscopic observations made with the Optical System for Imaging and low Resolution Integrated Spectroscopy, mounted on the 10.4 m Gran Telescopio CANARIAS, of two of these protostars. The spectrum of one of the objects shows evidence of accretion but not of outflows. In the latter object, the spectra show several emission lines indicating the presence of an actively accreting disk with outflow. We present estimates of the mass loss rate and the accretion rate from the disk, showing that the former exceeds the latter as observed in other known objects with evaporating disks. We also show evidence of a strong variability in the integrated flux observed in these objects as well as in the accretion and outflow diagnostics.

  13. BOREAS TGB-8 Monoterpene Concentration Data over the SSA-OBS

    NASA Technical Reports Server (NTRS)

    Hall, Forrest G. (Editor); Conrad, Sara K. (Editor); Lerdau, Manuel

    2000-01-01

    The BOREAS TGB-8 team collected data to investigate the controls over NMHC fluxes from boreal forest tree species. This data set contains measurements of monoterpene concentrations in collected foliar gas emissions and foliar samples. The data were collected at the OJP and OBS tower flux sites in the SSA and were the locus for the monoterpene emission measurements. These areas contained mature stands of jack pine and black spruce and were the focal sites in the BOREAS program for studies of biosphere/atmosphere exchange from these two habitat types. The OBS site is situated in a black spruce/sphagnum bog with the largest trees 155 years old and 10-15 m tall. The OJP site is in a jack pine forest, 80 to 120 years old, which lies on a sandy bench of glacial outwash with the largest tree standing 15 m tall. Temporally, the data cover the period of 24-May-1994 to 19-Sep-1994. The data are stored in tabular ASCII files.

  14. BOREAS TGB-8 Starch Concentration Data Over the SSA-OBS and the SSA-OJP

    NASA Technical Reports Server (NTRS)

    Lerdau, Manuel; Hall, Forrest G. (Editor); Conrad, Sara K. (Editor)

    2000-01-01

    The BOREAS TGB-8 team collected data to investigate the controls over NMHC fluxes from boreal forest tree species. This data set includes measurements of starch concentrations in foliar samples at mature jack pine and black spruce sites. The data were collected at the OJP and OBS tower flux locations in the BOREAS SSA. These areas contained mature stands of jack pine and black spruce and were the focal sites in the BOREAS program for studies of biosphere/atmosphere exchange from these two habitat types. The OBS site is situated in a black spruce/sphagnum bog with the largest trees 155 years old and 10-15 m tall. The OJP site is in a jack pine forest, 80 to 120 years old, which lies on a sandy bench of glacial outwash with the largest tree standing 15 m tall. Temporally, the data cover the period of 24-May-1994 to 19-Sep-1994. The data are stored in tabular ASCII files.

  15. EVIDENCE FOR DELAYED MASSIVE STAR FORMATION IN THE M17 PROTO-OB ASSOCIATION

    SciTech Connect

    Povich, Matthew S.; Whitney, Barbara A. E-mail: bwhitney@spacescience.or

    2010-05-10

    Through analysis of archival images and photometry from the Spitzer GLIMPSE and MIPSGAL surveys combined with Two Micron All Sky Survey and MSX data, we have identified 488 candidate young stellar objects (YSOs) in the giant molecular cloud M17 SWex, which extends {approx}50 pc southwest from the prominent Galactic H II region M17. Our sample includes >200 YSOs with masses >3 M {sub sun} that will become B-type stars on the main sequence. Extrapolating over the stellar initial mass function (IMF), we find that M17 SWex contains >1.3 x 10{sup 4} young stars, representing a proto-OB association. The YSO mass function is significantly steeper than the Salpeter IMF, and early O stars are conspicuously absent from M17 SWex. Assuming M17 SWex will form an OB association with a Salpeter IMF, these results reveal the combined effects of (1) more rapid circumstellar disk evolution in more massive YSOs and (2) delayed onset of massive star formation.

  16. The Adult Ventricular-Subventricular Zone (V-SVZ) and Olfactory Bulb (OB) Neurogenesis.

    PubMed

    Lim, Daniel A; Alvarez-Buylla, Arturo

    2016-01-01

    A large population of neural stem/precursor cells (NSCs) persists in the ventricular-subventricular zone (V-SVZ) located in the walls of the lateral brain ventricles. V-SVZ NSCs produce large numbers of neuroblasts that migrate a long distance into the olfactory bulb (OB) where they differentiate into local circuit interneurons. Here, we review a broad range of discoveries that have emerged from studies of postnatal V-SVZ neurogenesis: the identification of NSCs as a subpopulation of astroglial cells, the neurogenic lineage, new mechanisms of neuronal migration, and molecular regulators of precursor cell proliferation and migration. It has also become evident that V-SVZ NSCs are regionally heterogeneous, with NSCs located in different regions of the ventricle wall generating distinct OB interneuron subtypes. Insights into the developmental origins and molecular mechanisms that underlie the regional specification of V-SVZ NSCs have also begun to emerge. Other recent studies have revealed new cell-intrinsic molecular mechanisms that enable lifelong neurogenesis in the V-SVZ. Finally, we discuss intriguing differences between the rodent V-SVZ and the corresponding human brain region. The rapidly expanding cellular and molecular knowledge of V-SVZ NSC biology provides key insights into postnatal neural development, the origin of brain tumors, and may inform the development regenerative therapies from cultured and endogenous human neural precursors. PMID:27048191

  17. Clustering of OB-fold domains of the partner protease complexed with trimeric stomatin from Thermococcales.

    PubMed

    Yokoyama, Hideshi; Matsui, Eriko; Hiramoto, Kana; Forterre, Patrick; Matsui, Ikuo

    2013-07-01

    The C-terminal soluble domain of stomatin operon partner protein (STOPP) of the hyperthermophilic archaeon Pyrococcus horikoshii has an oligonucleotide binding-fold (OB-fold). STOPP lacks the conserved surface residues necessary for binding to DNA/RNA. A tryptophan (W) residue is conserved instead at the molecular surface. Solvent-accessible W residues are often found at interfaces of protein-protein complexes, which suggested the possibility of self-assembling of STOPP. Protein-protein interactions among the C-terminal soluble domains of STOPP PH1510 (1510-C) were then analyzed by chemical linking and blue native polyacrylamide gel electrophoresis (BN-PAGE) methods. These results suggest that the soluble domains of STOPP could assemble into homo-oligomers. Since hexameric subcomplex I from archaeal proteasome consists of coiled-coil segments and OB-fold domains, molecular modeling of 1510-C was performed using hexameric subcomplex I as a template. Although 1510-C is a comparatively small polypeptide consisting of approximately 60 residues, numerous salt bridges and hydrophobic interactions were observed in the predicted hexamer of 1510-C, suggesting the stability of the homo-oligomeric structure. This oligomeric property of STOPP may be favorable for triplicate proteolysis of the trimer of prokaryotic stomatin. PMID:23587725

  18. Parametrization of DFTB3/3OB for Magnesium and Zinc for Chemical and Biological Applications

    PubMed Central

    2015-01-01

    We report the parametrization of the approximate density functional theory, DFTB3, for magnesium and zinc for chemical and biological applications. The parametrization strategy follows that established in previous work that parametrized several key main group elements (O, N, C, H, P, and S). This 3OB set of parameters can thus be used to study many chemical and biochemical systems. The parameters are benchmarked using both gas-phase and condensed-phase systems. The gas-phase results are compared to DFT (mostly B3LYP), ab initio (MP2 and G3B3), and PM6, as well as to a previous DFTB parametrization (MIO). The results indicate that DFTB3/3OB is particularly successful at predicting structures, including rather complex dinuclear metalloenzyme active sites, while being semiquantitative (with a typical mean absolute deviation (MAD) of ∼3–5 kcal/mol) for energetics. Single-point calculations with high-level quantum mechanics (QM) methods generally lead to very satisfying (a typical MAD of ∼1 kcal/mol) energetic properties. DFTB3/MM simulations for solution and two enzyme systems also lead to encouraging structural and energetic properties in comparison to available experimental data. The remaining limitations of DFTB3, such as the treatment of interaction between metal ions and highly charged/polarizable ligands, are also discussed. PMID:25178644

  19. Into the Darkness: Interstellar Extinction Near the Cepheus OB3 Molecular Cloud

    NASA Astrophysics Data System (ADS)

    Fitzpatrick, Edward L.; Jacklin, S.; Massa, D.

    2014-01-01

    We present the results of a followup investigation to a study performed by Massa and Savage (1984, ApJ, 279, 310) of the properties of UV interstellar extinction in the region of the Cepheus OB3 molecular cloud. That study was performed using UV photometry and spectro-photometry from the ANS and IUE satellites. We have extended this study into the IR, utilizing the uniform database of IR photometry available from the 2MASS project. This is a part of a larger program whose goal is to study the properties of extinction in localized regions, where we hope to find clues to dust grain growth and destruction processes through spatial correlations of extinction with distinct environmental properties. Similarly to Massa and Savage’s UV results, we find that the IR extinction properties on the Cepheus OB3 region vary systematically with the apparent proximity of the target stars to the molecular cloud. We also find that the UV extinction and the IR extinction are crudely correlated. The methodology leading to these results and their implications are discussed.

  20. Methanotrophs, Methylosinus trichosporium OB3b, sMMO, and their application to bioremediation.

    PubMed

    Sullivan, J P; Dickinson, D; Chase, H A

    1998-01-01

    One of the most problematic groups of the USEPA and EU priority pollutants are the halogenated organic compounds. These substances have a wide range of industrial applications, such as solvents and cleaners. Inadequate disposal techniques and accidental spillages have led to their detection in soil, groundwater, and river sediments. Persistence of these compounds in the environment has resulted from low levels of biodegradation due to chemical structural features that preclude or retard biological attack. Research has indicated the idea that treatment systems based on methanotrophic co-metabolic transformation may be a cost-effective and efficient alternative to physical methods because of the potential for high transformation rates, the possibility of complete compound degradation without the formation of toxic metabolites, applicability to a broad spectrum of compounds, and the use of a widely available and inexpensive growth substrate. A substantial amount of work concerning methanotrophic cometabolic transformations has been carried out using the soluble form of methane monooxygenase (sMMO) from the obligate methanotroph Methylosinus trichosporium OB3b. This NADH-dependent monooxygenase is derepressed when cells are grown under copper stress. sMMO has a wider specificity than the particulate form. sMMO has been shown to degrade trichloroethylene (TCE) at a rate of at least one order of magnitude faster than obtained with other mixed and pure cultures, suggesting it has a wider application to bioremediation. Furthermore, sMMO catalyzes an unusually wide range of oxidation reactions, including the hydroxylation of alkanes, epoxidation of alkenes, ethers, halogenated methanes, cyclic and aromatic compounds including compounds, that are resistant to degradation in the environment. However, the practical application of methantrophs and Methylosinus trichosporium OB3b to the treatment of chlorinated organics has met with mixed success. Although oxidation rates are

  1. Ocean Basalt Simulator version 1 (OBS1): Trace element mass balance in adiabatic melting of a pyroxenite-bearing peridotite

    NASA Astrophysics Data System (ADS)

    Kimura, Jun-Ichi; Kawabata, Hiroshi

    2015-01-01

    present a new numerical trace element mass balance model for adiabatic melting of a pyroxenite-bearing peridotite for estimating mantle potential temperature, depth of melting column, and pyroxenite fraction in the source mantle for a primary ocean basalt/picrite. The Ocean Basalt Simulator version 1 (OBS1) uses a thermodynamic model of adiabatic melting of a pyroxenite-bearing peridotite with experimentally/thermodynamically parameterized liquidus-solidus intervals and source mineralogy. OBS1 can be used to calculate a sequence of adiabatic melting with two melting models, including (1) melting of peridotite and pyroxenite sources with simple mixing of their fractional melts (melt-melt mixing model), and (2) pyroxenite melting, melt metasomatism in the host peridotite, and melting of the metasomatized peridotite (source-metasomatism model). OBS1 can be used to explore (1) the fractions of peridotite and pyroxenite, (2) mantle potential temperature, (3) pressure of termination of melting, (4) degree of melting, and (5) residual mode of the sources. In order to constrain these parameters, the model calculates a mass balance for 26 incompatible trace elements in the sources and in the generated basalt/picrite. OBS1 is coded in an Excel spreadsheet and runs with VBA macros. Using OBS1, we examine the source compositions and conditions of the mid-oceanic ridge basalts, Loihi-Koolau basalts in the Hawaiian hot spot, and Jurassic Shatsky Rise and Mikabu oceanic plateau basalts and picrites. The OBS1 model shows the physical conditions, chemical mass balance, and amount of pyroxenite in the source peridotite, which are keys to global mantle recycling.

  2. Seismicity and Attenuation of the Continental Margin Off New York and Southern/ New England from OBS Data

    NASA Astrophysics Data System (ADS)

    Ten Brink, U. S.; Flores, C. H.; McGuire, J. J.; Collins, J. A.

    2014-12-01

    Earthquake data recorded during two ocean-bottom-seismometer (OBS) deployments on the continental slope off New York and Southern New England are used to evaluate micro-seismicity and attenuation of the continental margin. The deployments were partly motivated by the detection by land seismometers of several ML2.5-3.8 continental margin earthquakes in this region during the previous four years. The data come from a five-month-long cluster deployment (1 km radius) of eleven short-period OBS in July 2012 at a water depth of ~835 m and a subsequent nine-month deployment of a network of five short-period OBS spaced 50 km apart at water depths between 900-2400 m. The land-based seismic network did not identify seismic activity on the margin during the two deployments. The OBS network located only one earthquake of ML~1 near the shelf edge, suggesting that seismic activity of the margin is probably successfully monitored by land seismometers. A successful land-based detection of earthquakes on the margin may indicate that low seismic attenuation extends from the Eastern U.S. to the continental shelf and slope. To better quantify the attenuation of the margin we derive the peak spectral attenuation of 6-8 ML2.8-4.1 earthquakes recorded by the OBS during the two deployments. These earthquakes were located on land close to shore and offshore between New Jersey and Nova Scotia, and propagation paths are almost entirely within the continental margin. Onshore earthquakes of ML ≤2.6 were not detected by the OBS. Information about the seismicity and attenuation of the margin will be used to evaluate the probability of earthquake-generated landslides.

  3. The contribution and spatial distribution of Ob and Yenisei runoff on surface layer of the Kara Sea.

    NASA Astrophysics Data System (ADS)

    Polukhin, A.; Makkaveev, P.

    2012-04-01

    On degree of influence of river runoff on water area of the Kara Sea in general it is possible to consider as uniform estuary of two largest Siberian rivers - Ob and Yenisei. The Kara Sea has 41 % of all river runoff from a land in Arctic ocean or 56 % of a river runoff of the rivers of the Siberian sector of Arctic regions. From them of 37 % belong to waters from The Obskaya Guba (the Ob, the Taz, the Pur) and 46 % to waters of Yenisei. Spatial distribution of a river flow and its interaction with sea waters is in many respects defines various and changeable hydrometeorological conditions of the Kara Sea. Hydrochemical researches of the Kara Sea were included into the works of complex expedition in 59th cruise of R/V "Academic Mstislav Keldysh" (on September, 11th - on October, 7th, 2011). This data supplements results of expeditions of Institute of oceanology RAS to the Kara Sea in the autumn 1993 and 2007. In these cruises were met and described lenses of fresh water contained Ob and Yenisei waters defined on hydrochemical parameters. Difference of the data of 2011 from last years is that sampling for researches of distribution of river flow (on silicon, and the general alkalinity) was spent in flowing system from horizon of 1-1,5 m on a course of a vessel with high frequency of sampling. Such technique of sampling allows to investigate a surface water area with high discretness which plays the main role in definition of the contribution of waters of Ob and Yenisei in surface water layer of the Kara Sea. The analysis of the data shows that the area of distribution and the relative contribution of waters of a different origin considerably changes from year to year. It is connected with considerable interannual variability of hydrometeorological conditions and in particular with the general circulation of waters of the Kara Sea. River flow distribution on the surface of the Kara Sea is difficult enough. Nevertheless, distinctions in a chemical compound of waters

  4. Seafloor ground rotation observations: potential for improving signal-to-noise ratio on horizontal OBS components

    NASA Astrophysics Data System (ADS)

    Lindner, Fabian; Wassermann, Joachim; Schmidt-Aursch, Mechita; Schreiber, Ulrich; Igel, Heiner

    2015-04-01

    It is well known that the horizontal components of ocean bottom seismometer (OBS) records have a very poor signal-to-noise (S/N) ratio compared to the vertical components, the difference substantially exceeding that of terrestrial records. This is unfortunate as 1) OBS experiments are expensive and the main possibility to gather data in offshore areas, and 2) today we are more and more interested in modelling complete waveforms including all three components aiming at optimally constraining geophysical parameters for inverse problems for Earth's structure and seismic sources. Despite the fact that it is expected that tilting is the major cause of this high S/N - to our knowledge - this effect has never been directly observed. The reason is that (standard) instruments for the measurement of uncontaminated rotational ground motions with the required sensitivity still do not exist. Here, we report observations from an experiment we carried out in the North Sea, close to the island of Helgoland in the summer of 2014. A commercial fibre-optic gyro (usually used for navigation purposes) recording ground rotation rate with a sensitivity of approx. 10-7 rad/s was mounted on an OBS system together with a broadband seismometer. The system was lowered to the seafloor for about a week. To investigate a potential connection between rotational ground motions around the two horizontal axes (i.e., tilting) we calculate the coherence between the corresponding motion components (e.g., rotations around x-axis and translational motions along y-axis, and vice versa). We find very high correlations, on average exceeding 0.73 in the period interval 7-13 seconds. Correlations seem to increase with noise amplitude. Rotation rate amplitudes are in the range of 10-6 -10-5 rad/s. This clearly indicates that the horizontal translational components are severely contaminated by rotations around the horizontal axes. The ground rotation observations allow correcting for this effect thereby

  5. The hyperleptinemia and ObRb expression in hyperphagic obese rats.

    PubMed

    Han, Ying; Joe, Yeonsoo; Seo, Eunhui; Lee, Sa Rah; Park, Mi-Kyoung; Lee, Hye-Jeong; Kim, Duk Kyu

    2010-03-26

    Leptin resistance associated with hyperleptinemia in high-fat-diet-induced obese rats and aged obese rats is well established, but it is not clear whether hyperphagia-induced obese rats also develop leptin resistance. We investigated whether Otsuka Long-Evans Tokushima Fatty (OLETF) rats, which are a strain of hyperphagia-induced obese rats, develop leptin resistance and whether caloric restriction reversed this leptin resistance-induced leptin receptor (ObRb) deficit. Twenty male OLETF rats, 20 male Long-Evans Tokushima Otsuka (LETO) rats, and 10 male Sprague Dawley (SD) rats were used. All rats were initially studied at 10 weeks of age and were freely fed with standard rat chow and water until they were 38 weeks of age. Daily food intake, body weight, and plasma leptin levels of OLETF rats were remarkably increased compared to LETO or SD rats from 10 to 38 weeks of age. When they were 38 weeks of age, all OLETF rats were randomly divided into two groups. One group was freely fed with standard rat chow (FD, or free diet group), and the other group (RD, or restricted diet group) was fed with only 70% of the amount consumed by the FD group. The LETO and SD rats were dismissed from further study. After 4 weeks of caloric restriction, the average body weight (636+/-33 g vs. 752+/-24 g, P<0.05) and abdominal adipose tissue weight (10.6+/-3.2g vs. 15.8+/-1.5 g, P<0.05) of the RD group were decreased compared with those of the FD group. Plasma leptin levels of the RD group were significantly decreased compared with those of the FD group (3.47+/-1.40 ng/mL vs. 11.55+/-1.16 ng/mL, P<0.05). The mRNA expression of ObRb and leptin-related suppressor of cytokine signaling 3 (SOCS3) in the hypothalamus, liver, and skeletal muscles of the RD group were significantly decreased compared with those of the FD group. Caloric restriction did not improve leptin receptor (ObRb) deficit or the downstream signaling of leptin in the liver, skeletal muscles, and hypothalamus. Thus, we

  6. Trace metals in the Ob and Yenisei Rivers' Estuaries (the Kara Sea).

    NASA Astrophysics Data System (ADS)

    Demina, L. L.

    2014-12-01

    Behavior of some trace metals (Al, As, Cd, Co, Cr, Cu, Fe, Mn, Ni and Pb) in water column (soluble <0.45 µm and particulate fractions) and bottom sediments (surface and cores) along the two transects from the Ob River and Yenisei River Estuaries to the Kara Sea was studied. The length of both transects was about 700 km. Water depth was 12-63 m, O2 dissolved :5.36-9.55 ml l-1. Along the transects salinity increased from 0.07 to 34.2 psu, while the SPM' concentration decreased from 10.31 to 0.31 mg/l. Total suspended particulate matter load is more than one order of magnitude higher in the Ob River Estuary comparing to that of the Yenisei River. It has led to a significant difference between the suspended trace metals' concentrations (µg/l) in water of the two estuaries. With salinity increase along transects Fe susp., Mn susp. and Zn susp. decreased by a factor of 100-500, that has led to a growth of a relative portion of dissolved trace metals followed by their bioaccumulation (Demina et al., 2010). A strong direct correlation between suspended Cu, Fe and SPM mass concentration was found. For the first time along the Yenisei River' Estuary -the Kara Sea transect a direct positive correlation between Cu suspended and volume concentration of SPM (mg/ml3) was found, that was attributed to contribution of phytoplankton aggregates in the SPM composition. A trend of relationship between content of suspended As and pelitic fraction (2-10 µm) of SPM was firstly found in theses basins also. Study of trace metal speciation in the bottom sediments (adsorbed, associated with Fe-Mn (oxyhydr)oxides, organic matter and fixed in the mineral lattice or refractory) has revealed the refractory fraction to be prevailing (70-95% total content) for Fe, Zn, Cu, Co, Ni, Cr, Cd and Pb. That means that toxic heavy metals were not available for bottom fauna. Mn was predominantly found in the adsorbed and (oxyhydr)oxides geochemically labile forms, reflecting the redox condition change

  7. Islet adaptation to obesity and insulin resistance in WNIN/GR-Ob rats

    PubMed Central

    Singh, Himadri; Ganneru, Sireesha; Malakapalli, Venkata; Chalasani, Maniprabha; Nappanveettil, Giridharan; Bhonde, Ramesh R; Venkatesan, Vijayalakshmi

    2014-01-01

    WNIN/GR-Ob mutant rat is a novel animal model to study metabolic syndrome (obesity, insulin resistance, hyperinsulinemia, impaired glucose tolerance and cardiovascular diseases). We have investigated the islet characteristics of obese mutants at different age groups (1, 6 and 12 months) to assess the islet changes in response to early and chronic metabolic stress. Our data demonstrates altered islet cell morphology and function (hypertrophy, fibrotic lesions, vacuolation, decreased stimulation index, increased TNFα, ROS and TBARS levels) in mutants as compared to controls. Furthermore, network analysis (gene-gene interaction) studied in pancreas demonstrated increased inflammation as a key factor underlying obesity/metabolic syndrome in mutants. These observations pave way to explore this model to understand islet adaptation in response to metabolic syndrome. PMID:25833252

  8. A FORMATION SCENARIO OF YOUNG STELLAR GROUPS IN THE REGION OF THE SCORPIO CENTAURUS OB ASSOCIATION

    SciTech Connect

    Ortega, V. G.; Jilinski, E.; De la Reza, R.; Bazzanella, B.

    2009-04-15

    The main objective of this work is to investigate the role played by Lower Centaurus Crux (LCC) and Upper Centaurus Lupus (UCL), both subcomponents of the Scorpio Centaurus OB association (Sco-Cen), in the formation of the groups {beta} Pictoris, TW Hydrae, and the {eta} Chamaeleontis cluster. The dynamical evolution of all the stellar groups involved and of the bubbles and shells blown by LCC and UCL are calculated, and followed from the past to the present. This leads to a formation scenario in which (1) the groups {beta} Pictoris, TW Hydrae were formed in the wake of the shells created by LCC and UCL, (2) the young cluster {eta} Chamaeleontis was born as a consequence of the collision of the shells of LCC and UCL, and (3) the formation of Upper Scorpius (US), the other main subcomponent of the Sco-Cen association, may have been started by the same process that created {eta} Chamaeleontis.

  9. Degradation of Trichloroethylene by Methanol-Grown Cultures of Methylosinus trichosporium OB3b PP358

    PubMed Central

    Fitch, M. W.; Speitel, G. E.; Georgiou, G.

    1996-01-01

    A soluble methane monooxygenase-constitutive mutant strain of Methylosinus trichosporium OB3b, strain PP358, was grown with methanol as the carbon source, and the kinetics of trichloroethylene (TCE) degradation were determined. PP358 exhibited high TCE degradation rates under both oxygen- and carbon-limiting conditions. The optimal pseudo first-order rate constant for TCE was comparable to the values measured for cells grown with methane. We found that growth under oxygen-limiting conditions results in increased accumulation of polyhydroxybutyrate, which in turn correlates with higher transformation capacities for TCE. It was also shown that methanol inhibits TCE degradation only at high concentrations. Thus, methanol-grown cultures of PP358 represent an efficient system for the biodegradation of chlorinated hydrocarbons. PMID:16535263

  10. A multiwavelength investigation of the massive eclipsing binary Cygnus OB2 #5

    NASA Astrophysics Data System (ADS)

    Linder, N.; Rauw, G.; Manfroid, J.; Damerdji, Y.; De Becker, M.; Eenens, P.; Royer, P.; Vreux, J.-M.

    2009-02-01

    Context: The properties of the early-type binary Cyg OB2 #5 have been debated for many years and spectroscopic and photometric investigations yielded conflicting results. Aims: We have attempted to constrain the physical properties of the binary by collecting new optical and X-ray observations. Methods: The optical light curves obtained with narrow-band continuum and line-bearing filters are analysed and compared. Optical spectra are used to map the location of the He ii λ 4686 and Hα line-emission regions in velocity space. New XMM-Newton as well as archive X-ray spectra are analysed to search for variability and constrain the properties of the hot plasma in this system. Results: We find that the orbital period of the system slowly changes though we are unable to discriminate between several possible explanations of this trend. The best fit solution of the continuum light curve reveals a contact configuration with the secondary star being significantly brighter and hotter on its leading side facing the primary. The mean temperature of the secondary star turns out to be only slightly lower than that of the primary, whilst the bolometric luminosity ratio is found to be 3.1. The solution of the light curve yields a distance of 925 ± 25 pc much lower than the usually assumed distance of the Cyg OB2 association. Whilst we confirm the existence of episodes of higher X-ray fluxes, the data reveal no phase-locked modulation with the 6.6 day period of the eclipsing binary nor any clear relation between the X-ray flux and the 6.7 yr radio cycle. Conclusions: The bright region of the secondary star is probably heated by energy transfer in a common envelope in this contact binary system as well as by the collision with the primary's wind. The existence of a common photosphere probably also explains the odd mass-luminosity relation of the stars in this system. Most of the X-ray, non-thermal radio, and possibly γ-ray emission of Cyg OB2 #5 is likely to arise from the

  11. OB associations and the nonuniversality of the cosmic abundances - Implications for cosmic rays and meteorites

    NASA Technical Reports Server (NTRS)

    Olive, K. A.; Schramm, D. N.

    1982-01-01

    The formation of the solar system inside an OB association is examined with particular attention to the elemental abundances which would have been ejected by the association's first few supernovae. It is found that the solar system material may have been significantly contaminated by these supernovae and thus the average interstellar composition may differ from the solar system composition. In particular, we find that many of the so-called isotopic and elemental abundance anomalies (e.g., Ne, C, O, s-process/r-process, etc.) found in meteoritic inclusions and in cosmic rays may be more representative of the average interstellar abundance. In other words, it may be that the average solar system abundances are what is 'anomalous'.

  12. BOREAS RSS-17 Xylem Flux Density Measurements at the SSA-OBS Site

    NASA Technical Reports Server (NTRS)

    Zimmerman, Reiner; Way, JoBea; McDonald, Kyle; Nickeson, Jaime (Editor); Hall, Forrest G. (Editor); Smith, David E. (Technical Monitor)

    2000-01-01

    As part of its efforts to determine environmental and phenological states from radar imagery, the Boreal Ecosystem-Atmosphere Study (BOREAS) Remote Sensing Science (RSS)-17 team collected in situ tree xylem flow measurements for one growing season on five Picea mariana (black spruce) trees. The data were collected to obtain information on the temporal and spatial variability in water uptake by trees in the Southern Study Area-Old Black Spruce (SSA-OBS) stand in the BOREAS SSA. Temporally, the data were collected in 30-minute intervals for 120 days from 31 May 1994 until 27 September 1994. The data are stored in tabular ASCII files. The xylem flux data are available from the Earth Observing System Data and Information System (EOSDIS) Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC). The data files are available on a CD-ROM (see document number 20010000884).

  13. A photometric study of the Orion OB 1 association. 1: Observational data

    NASA Technical Reports Server (NTRS)

    Warren, W. H., Jr.; Hesser, J. E.

    1976-01-01

    An extensive catalog of observational data is presented for stars in the region of the young stellar association Orion OB 1. In addition to new photoelectric observations obtained on the uvbyB and UBV systems, photoelectric and spectroscopic data were compiled for the stars observed and for several bright members of the association having available photometric indices. Mean weighted values were computed for the uvbyB and UBV data and are tabulated in summary tables which include all references for individual values. These tables are expected to be reasonably complete for association members earlier than spectral type A0. From an analysis of currently available proper motion, radial velocity, and photometric data, membership criteria were derived and qualitative membership probabilities for 526 stars were summarized. A set of charts is included for assistance in identification of the program stars in all regions of the association.

  14. Search for old neutron stars in molecular clouds: Cygnus rift and Cygnus OB7.

    NASA Astrophysics Data System (ADS)

    Belloni, T.; Zampieri, L.; Campana, S.

    1997-03-01

    We present the results of a systematic search for old isolated neutron stars (ONSs) in the direction of two giant molecular clouds in Cygnus (Rift and OB7). From theoretical calculations, we expect the detection of a large number of ONSs with the PSPC on board ROSAT. By analyzing the PSPC pointings in the direction of the clouds, we find four sources characterized by count rates (~10^-3^ct/s) and spectral properties consistent with the hypothesis that the X-ray radiation is produced by ONSs and also characterized by the absence of any measurable optical counterpart within their error circle in the digitized red plates of the Palomar All Sky Survey. The importance of follow-up deep observations in the direction of these ONS candidates is discussed. The observational and theoretical approach presented here could be fruitfully applied also to the systematic search for ONSs in other regions of the Galaxy.

  15. Statistical study of OB stars in NGC 6334 and NGC 6357

    NASA Astrophysics Data System (ADS)

    Russeil, D.; Zavagno, A.; Adami, C.; Anderson, L. D.; Bontemps, S.; Motte, F.; Rodon, J. A.; Schneider, N.; Ilmane, A.; Murphy, K. J.

    2012-02-01

    Context. Star-forming complexes are large structures exhibiting massive star-formation at different stages of evolution, from dense cores to well-developed H ii regions. They are very interesting for the study of the formation and evolution of stars. NGC 6334 and NGC 6357 are two active and relatively nearby star-forming complexes. From the extinction map and the sub-mm cold dust emission, and because they have similar velocities, these regions are most likely connected. However, located in the direction of the Galactic center their radial velocity is not representative of their distance. An alternative is then to determine the distance of NGC 6334 and NGC 6357 from their stellar content. Aims: Our aim is to perform a census of O-B3 ionising stars in NGC 6334 and NGC 6357, to determine the extinction coefficient, and the distance of both regions. A census of O-B3 stars is an essential basis for estimating the statistical lifetime of the earliest massive star-forming phases. Methods: We performed a U, B, V, and R photometric survey of a large area covering NGC 6334 and NGC 6357 with the VIMOS (ESO-VLT) and the MOSAIC (CTIO) instruments. This allows us to have a complete census of O to B3 stars up to V = 22.6 mag. The OB stars are selected based on their U - B and B - V colors. The most robust extinction coefficient is determined from color - color plots before computing the distance of the OB stars. Results: We find a higher value than typical of the diffuse interstellar medium for RV of 3.53 ± 0.08 and 3.56 ± 0.15 for NGC 6357 and NGC 6334, respectively. Adopting these RV values, the distances of NGC 6357 and NGC 6334 are 1.9 ± 0.4 kpc and 1.7 ± 0.3 kpc. We conclude that, within the error bars, both regions are thus at the same distance of 1.75 kpc (weighted mean). We confirm that the value of RV is linked to the large dust grain content. In particular, we found that there are more very small grains in NGC 6357 than in NGC 6334, suggesting that NGC 6357 could

  16. Les aspects psychosociaux de l’obésité chez les enfants et les adolescents

    PubMed Central

    Nieman, Peter; LeBlanc, Claire MA

    2012-01-01

    RÉSUMÉ En plus de donner des conseils aux familles au sujet de l’activité physique régulière et d’une saine alimentation, les cliniciens doivent déterminer les facteurs psychosociaux qui contribuent à l’obésité des enfants ou des adolescents et les aider à y faire face. Les personnes touchées peuvent souffrir de dépression, de mauvaise estime de soi, d’intimidation et de préjugés liés au poids, qui sont tous des expériences qui peuvent compliquer l’obtention des résultats de santé souhaités. Les cliniciens devraient tenter de déterminer les facteurs stressants sous-jacents et s’assurer de la mise en œuvre de conseils pertinents.

  17. VizieR Online Data Catalog: Interstellar extinction curves of OB stars (Wegner 2002)

    NASA Astrophysics Data System (ADS)

    Wegner, W.

    2003-02-01

    The paper presents a collection of 436 extinction curves covering the whole available range of wavelengths from satellite UV to near-IR. The data were taken from the ANS photometric catalogue (Cat. ) and from the compilations of IR photometric measurements. The data curves have been obtained with the aid of "artificial standards" Papaj et al. (1993A&A...273..575P) and Wegner (1994MNRAS.270..229W, 1995, Interstellar Absorption Structures in the Direction of Nearby OB stars, Wyd. Uczelniane WSP, Bydgoszcz, p. 1-383). The visual magnitudes and spectral classifications of O and B type stars with EB-V>=0.05 were taken from the SIMBAD database. The curves are given in the form of plots and tables E{lambda}-V/EB-V versus 1/{lambda}. The observed variety of extinction laws among slightly reddened stars is apparently due to the various physical parameters of interstellar clouds. (3 data files).

  18. Near-Infrared Stellar Photometry of the M31 Spiral Arm around OB Association A24

    NASA Astrophysics Data System (ADS)

    Kodaira, Keiichi; Miyazaki, Satoshi; Vansevičius, Vladas; Tamura, Motohide; Tokunaga, Alan; Kobayashi, Naoto

    1998-09-01

    Deep high-resolution JHK images of a 2' × 2' field around the OB association A24 near the 7 kpc spiral arm in M31 were measured to yield photometric data of more than 3000 stars with the faintest magnitude being J = 21.3, H = 20.5, and K = 19.6. The photometric data for the 1037 stars that are brightest in the K band are presented. Judging from the color-magnitude and the two-color diagrams, we find that the majority of the observed stars are AGB stars including large-amplitude variables. They are distributed smoothly over the observed field, and we find no clear evidence of varying extinction over the field for these red stars. The present sample includes bright AGB stars of MK <= -8 and about 30 young blue objects of (J-H) < 0.75 that are brighter than MJ ~= -6.5.

  19. MODELING THE RADIO EMISSION FROM Cyg OB2 NO. 5: A QUADRUPLE SYSTEM?

    SciTech Connect

    Kennedy, M.; Dougherty, S. M.; Fink, A.; Williams, P. M. E-mail: sean.dougherty@nrc.c E-mail: pmw@roe.ac.u

    2010-02-01

    Fifty observations at frequencies between 1.4 GHz and 43 GHz of the 6.6 day O6.5-7+O5.5-6 binary Cyg OB2 No. 5 using the Very Large Array over 20 years are re-examined. The aim is to determine the location and character of the previously detected variable radio emission. The radio emission from the system consists of a primary component that is associated with the binary, and a non-thermal source (NE), 0.''8 to the NE of the binary that has been ascribed to a wind-collision region (WCR) between the stellar winds of the binary and that of a B-type star (Star D) to the NE. Previous studies have not accounted for the potential contribution of NE to the total radio emission, most especially in observations where the primary and NE sources are not resolved as separate sources. NE shows no evidence of variation in 23 epochs where it is resolved separately from the primary radio component, demonstrating that the variable emission arises in the primary component. Since NE is non-variable, the radio flux from the primary can now be well determined for the first time, most especially in observations that do not resolve both the primary and NE components. The variable radio emission from the primary component has a period of 6.7 +- 0.3 years which is described by a simple model of a non-thermal source orbiting within the stellar wind envelope of the binary. Such a model implies the presence of a third, unresolved stellar companion (Star C) orbiting the 6.6 day binary with a period of 6.7 years and independent of Star D to the NE. The variable non-thermal emission arises from either a WCR between Star C and the binary system, or possibly from Star C directly. The model gives a mass-loss rate of 3.4 x 10{sup -5} M{sub sun} yr{sup -1} for Cyg OB2 No. 5, unusually high for an Of supergiant and comparable to that of WR stars, and consistent with an unusually strong He I 1.083 mum emission line, also redolent of WR stars. An examination of radial velocity observations available

  20. A spectacular molecular outflow in the Monoceros OB1 molecular cloud

    NASA Technical Reports Server (NTRS)

    Margulis, Michael; Lada, Charles J.; Hasegawa, Tetsuo; Hayashi, Saeko S.; Hayashi, Masihiko

    1990-01-01

    Detailed observations of CO, CS, IR continuum, and H2 emission from a large, highly collimated, bipolar outflow in the Monoceros OB1 molecular cloud are presented. The CO observations suggest that molecular gas in the outflow is contained in a shell with higher velocity material situated interior to lower velocity material. The velocities of outflow emission are found to increase with increasing distance from the center of the outflow. Additional detections include shock-excited molecular hydrogen emission from the blueshifted lobe of the outflow and six 2-micron sources in the direction of the outflow. Near-IR and IRAS observations suggest that the driving source for the outflow must have a bolometric luminosity below about 4.5 solar luminosities. It is concluded that the flow is probably not driven by stellar radiation from a central source.

  1. Low energy gamma ray emission from the Cygnus OB2 association

    NASA Technical Reports Server (NTRS)

    Chen, Wan; White, Richard L.

    1992-01-01

    According to our newly developed model of gamma-ray emission from chaotic early-type stellar winds, we predict the combined gamma-ray flux from the circumstellar winds of many very luminous early-type stars in the Cyg OB2 association can be detectable by the Energetic Gamma Ray Experiment Telescope (EGRET) (and maybe also by OSSE) on CGRO. Due to different radiation mechanisms, the gamma-ray spectrum from stellar winds can be quite different from that of CYG X-3; this spectral difference and the time-variation of Cyg X-3 flux will help to distinguish the gamma-ray components from different sources in this small region, which is spatially unresolvable by CGRO.

  2. SCUBA and HIRES Results for Protostellar Cores in the MON OB1 Dark Cloud

    NASA Astrophysics Data System (ADS)

    Wolf-Chase, G.; Moriarty-Schieven, G.; Fich, M.; Barsony, M.

    1999-05-01

    We have used HIRES-processing of IRAS data and point-source modelling techniques (Hurt & Barsony 1996; O'Linger 1997; Barsony et al. 1998), together with submillimeter continuum imaging using the Submillimeter Common-User Bolometer Array (SCUBA) on the 15-meter James Clerk Maxwell Telescope (JCMT), to search CS cores in the Mon OB1 dark cloud (Wolf-Chase, Walker, & Lada 1995; Wolf-Chase & Walker 1995) for deeply embedded sources. These observations, as well as follow-up millimeter photometry at the National Radio Astronomy Observatory (NRAO) 12-meter telescope on Kitt Peak, have lead to the identification of two Class 0 protostellar candidates, which were previously unresolved from two brighter IRAS point sources (IRAS 06382+0939 & IRAS 06381+1039) in this cloud. Until now, only one Class 0 object had been confirmed in Mon OB1; the driving source of the highly-collimated outflow NGC 2264 G (Ward-Thompson, Eiroa, & Casali 1995; Margulis et al. 1990; Lada & Fich 1996), which lies well outside the extended CS cores. One of the new Class 0 candidates may be an intermediate-mass source associated with an H_2O maser, and the other object is a low-mass source which may be associated with a near-infrared jet, and possibly with a molecular outflow. We report accurate positions for the new Class 0 candidates, based on the SCUBA images, and present new SEDs for these sources, as well as for the brighter IRAS point sources. A portion of this work was performed while GWC held a President's Fellowship from the University of California. MB and GWC gratefully acknowledge financial support from MB's NSF CAREER Grant, AST97-9753229.

  3. Cerium regulates expression of alternative methanol dehydrogenases in Methylosinus trichosporium OB3b.

    PubMed

    Farhan Ul Haque, Muhammad; Kalidass, Bhagyalakshmi; Bandow, Nathan; Turpin, Erick A; DiSpirito, Alan A; Semrau, Jeremy D

    2015-11-01

    Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a "copper switch." At low copper/biomass ratios the soluble methane monooxygenase (sMMO) is expressed while expression and activity of the particulate methane monooxygenase (pMMO) increases with increasing availability of copper. In many methanotrophs there are also multiple methanol dehydrogenases (MeDHs), one based on Mxa and another based on Xox. Mxa-MeDH is known to have calcium in its active site, while Xox-MeDHs have been shown to have rare earth elements in their active site. We show here that the expression levels of Mxa-MeDH and Xox-MeDH in Methylosinus trichosporium OB3b significantly decreased and increased, respectively, when grown in the presence of cerium but the absence of copper compared to the absence of both metals. Expression of sMMO and pMMO was not affected. In the presence of copper, the effect of cerium on gene expression was less significant, i.e., expression of Mxa-MeDH in the presence of copper and cerium was slightly lower than in the presence of copper alone, but Xox-MeDH was again found to increase significantly. As expected, the addition of copper caused sMMO and pMMO expression levels to significantly decrease and increase, respectively, but the simultaneous addition of cerium had no discernible effect on MMO expression. As a result, it appears Mxa-MeDH can be uncoupled from methane oxidation by sMMO in M. trichosporium OB3b but not from pMMO. PMID:26296730

  4. Cerium Regulates Expression of Alternative Methanol Dehydrogenases in Methylosinus trichosporium OB3b

    PubMed Central

    Farhan Ul Haque, Muhammad; Kalidass, Bhagyalakshmi; Bandow, Nathan; Turpin, Erick A.; DiSpirito, Alan A.

    2015-01-01

    Methanotrophs have multiple methane monooxygenases that are well known to be regulated by copper, i.e., a “copper switch.” At low copper/biomass ratios the soluble methane monooxygenase (sMMO) is expressed while expression and activity of the particulate methane monooxygenase (pMMO) increases with increasing availability of copper. In many methanotrophs there are also multiple methanol dehydrogenases (MeDHs), one based on Mxa and another based on Xox. Mxa-MeDH is known to have calcium in its active site, while Xox-MeDHs have been shown to have rare earth elements in their active site. We show here that the expression levels of Mxa-MeDH and Xox-MeDH in Methylosinus trichosporium OB3b significantly decreased and increased, respectively, when grown in the presence of cerium but the absence of copper compared to the absence of both metals. Expression of sMMO and pMMO was not affected. In the presence of copper, the effect of cerium on gene expression was less significant, i.e., expression of Mxa-MeDH in the presence of copper and cerium was slightly lower than in the presence of copper alone, but Xox-MeDH was again found to increase significantly. As expected, the addition of copper caused sMMO and pMMO expression levels to significantly decrease and increase, respectively, but the simultaneous addition of cerium had no discernible effect on MMO expression. As a result, it appears Mxa-MeDH can be uncoupled from methane oxidation by sMMO in M. trichosporium OB3b but not from pMMO. PMID:26296730

  5. Winds of very low metallicity OB stars: crossing the frontier of the Magellanic Clouds

    NASA Astrophysics Data System (ADS)

    Garcia, Miriam

    2011-10-01

    Very low metallicity massive stars are a key ingredient for our understanding of the early Universe because of their connection with the dominant conditions at that time, the reionization epoch and long-GRBs. In the studies of massive stars radiation driven winds play a crucial manifold role, being a chief agent of stellar evolution, altering the optical diagnostics for parameter determination and injecting radiative and mechanical energy into their surroundings. However, the theory of radiation driven winds has only be tested down to SMC metallicities and some important open questions remain: the existence of solar-metallicity stars with weak winds and very recent evidence of relatively strong winds in metal-poor stars.We have secured VLT optical spectra of a sample of early-type massive stars in IC 1613, a very metal poor { <0.1Zo} irregular galaxy of the Local Group that represents the next step towards low metallicities after the SMC. We request low resolution COS spectra {COS/FUV-G140L} of a sub-set of OB stars probing different wind regimes. The wind lines in the 1150-1800A range, together with the optical spectra, will allow us to derive consistently the photospheric and wind parameters of the sample. Results will be interpreted in the context of both evolutionary and radiatively driven winds theories, testing the current paradigm at unexplored low metallicities and increasing our knowledge of massive stars under conditions closer to those of the deep Universe.COS enhanced sensitivity will allow us to perform for the first time detailed studies of **resolved** OB stars in an environment with poorer metal content than the SMC.

  6. Improving a modelling of the Ob river, in western Siberia, by assimilating wide swath altimetry data

    NASA Astrophysics Data System (ADS)

    Biancamaria, S.; Durand, M. T.; Andreadis, K.; Bates, P. D.; Boone, A.; Mognard, N. M.; Rodriguez, E.; Alsdorf, D. E.; Lettenmaier, D. P.

    2009-12-01

    Arctic rivers are a main source of fresh water to the Arctic Ocean: they represent nearly 10% of the world’s river runoff, whereas the Arctic Ocean represents only 6% of the global ocean surface area. Therefore, changes in their hydrological regime due to global warming could impact not only the thermohaline circulation, but also the carbon cycle (via their floodplains) and the human societies living in high latitudes. Thus, being able to model major arctic rivers and therefore predict how they may respond to global warming is a crucial issue. The upcoming Surface Water and Ocean Topography (SWOT) satellite mission will use a wide swath Ka-band Radar Interferometer (KaRIN) to acquire elevations of terrestrial water surfaces at unprecedented spatial and temporal resolutions, and will observe all continental water bodies between 78°N and 78°S. These measurements will be of broad interest for the hydrology community in general and for hydrologic/hydraulic modelling in particular. SWOT measurements will explicitly characterize the spatial and temporal surface water variations; thus, assimilating SWOT measurements into hydraulic models has great potential for estimating river discharge. This study presents hydrological modelling of the lower Ob river, in Western Siberia. The Ob is the third largest arctic river and contributes to nearly 15% of the total freshwater flow into the Arctic Ocean. The hydrological modelling has been performed by coupling the land surface scheme ISBA (Interactions between Soil, Biosphere and Atmosphere) with the flood inundation model LISFLOOD-FP. Virtual SWOT data have been computed within the context of a synthetic data assimilation experiment. We use the Ensemble Kalman Filter and Smoother to better assess the potential of the SWOT measurements to provide information about river discharge. Preliminary results show that these measurements could decrease the modelling errors almost to 50% for the nominal satellite orbit. Yet, these

  7. Distribution of global fallouts cesium-137 in taiga and tundra catenae at the Ob River basin

    NASA Astrophysics Data System (ADS)

    Semenkov, I. N.; Usacheva, A. A.; Miroshnikov, A. Yu.

    2015-03-01

    The classification of soil catenae at the Ob River basin is developed and applied. This classification reflects the diverse geochemical conditions that led to the formation of certain soil bodies, their combinations and the migration fields of chemical elements. The soil and geochemical diversity of the Ob River basin catenae was analyzed. The vertical and lateral distribution of global fallouts cesium-137 was studied using the example of the four most common catenae types in Western Siberia tundra and taiga. In landscapes of dwarf birches and dark coniferous forests on gleysols, cryosols, podzols, and cryic-stagnosols, the highest 137Cs activity density and specific activity are characteristic of the upper soil layer of over 30% ash, while the moss-grass-shrub cover is characterized by low 137Cs activity density and specific activity. In landscapes of dwarf birches and pine woods on podzols, the maximum specific activity of cesium-137 is typical for moss-grass-shrub cover, while the maximum reserves are concentrated in the upper soil layer of over 30% ash. Bog landscapes and moss-grass-shrub cover are characterized by a minimum activity of 137Cs, and its reserves in soil generally decrease exponentially with depth. The cesium-137 penetration depth increases in oligotrophic histosols from northern to middle taiga landscapes from 10-15 to 40 cm. 137Cs is accumulated in oligotrophic histosols for increases in pH from 3.3 to 4.0 and in concretionary interlayers of pisoplinthic-cryic-histic-stagnosols. Cryogenic movement, on the one hand, leads to burying organic layers enriched in 137Cs and, on the other hand, to deducing specific activity when mixed with low-active material from lower soil layers.

  8. Colliding filaments and a massive dense core in the Cygnus OB 7 molecular cloud

    SciTech Connect

    Dobashi, Kazuhito; Shimoikura, Tomomi; Akisato, Ko; Ohashi, Kenjiro; Nakagomi, Keisuke; Matsumoto, Tomoaki; Saito, Hiro

    2014-12-10

    We report the results of molecular line observations carried out toward a massive dense core in the Cyg OB 7 molecular cloud. The core has an extraordinarily large mass (∼1.1 × 10{sup 4} M {sub ☉}) and size (∼2 × 5 pc{sup 2}), but there is no massive young star forming therein. We observed this core in various molecular lines such as C{sup 18}O(J = 1-0) using the 45 m telescope at Nobeyama Radio Observatory. We find that the core has an elongated morphology consisting of several filaments and core-like structures. The filaments are massive (10{sup 2}-10{sup 3} M {sub ☉}), and they are apparently colliding with one another. Some candidates for young stellar objects are distributed around their intersection, suggesting that the collisions of the filaments may have influenced their formation. To understand the formation and evolution of such colliding filaments, we performed numerical simulations using the adaptive mesh refinement technique, adopting the observed core parameters (the mass and size) as the initial conditions. The results indicate that the filaments are formed as seen in other earlier simulations for small cores in the literature, but we could not reproduce the collisions of the filaments simply by assuming a large initial mass and size. We find that collisions of the filaments occur only when there is a large velocity gradient in the initial core, in a sense compressing it. We suggest that the observed core was actually compressed by an external effect, e.g., shocks from nearby supernova remnants, including HB 21 which has been suggested to be interacting with the Cyg OB 7 molecular cloud.

  9. On the observability of bow shocks of Galactic runaway OB stars

    NASA Astrophysics Data System (ADS)

    Meyer, D. M.-A.; van Marle, A.-J.; Kuiper, R.; Kley, W.

    2016-06-01

    Massive stars that have been ejected from their parent cluster and supersonically sailing away through the interstellar medium (ISM) are classified as exiled. They generate circumstellar bow-shock nebulae that can be observed. We present two-dimensional, axisymmetric hydrodynamical simulations of a representative sample of stellar wind bow shocks from Galactic OB stars in an ambient medium of densities ranging from nISM = 0.01 up to 10.0 cm- 3. Independently of their location in the Galaxy, we confirm that the infrared is the most appropriated waveband to search for bow shocks from massive stars. Their spectral energy distribution is the convenient tool to analyse them since their emission does not depend on the temporary effects which could affect unstable, thin-shelled bow shocks. Our numerical models of Galactic bow shocks generated by high-mass ( ≈ 40 M⊙) runaway stars yield H α fluxes which could be observed by facilities such as the SuperCOSMOS H-Alpha Survey. The brightest bow-shock nebulae are produced in the denser regions of the ISM. We predict that bow shocks in the field observed at H α by means of Rayleigh-sensitive facilities are formed around stars of initial mass larger than about 20 M⊙. Our models of bow shocks from OB stars have the emission maximum in the wavelength range 3 ≤ λ ≤ 50 μm which can be up to several orders of magnitude brighter than the runaway stars themselves, particularly for stars of initial mass larger than 20 M⊙.

  10. Effects of leptin on in vitro maturation, fertilization and embryonic cleavage after ICSI and early developmental expression of leptin (Ob) and leptin receptor (ObR) proteins in the horse

    PubMed Central

    Lange Consiglio, Anna; Dell'Aquila, Maria Elena; Fiandanese, Nadia; Ambruosi, Barbara; Cho, Yoon S; Bosi, Giampaolo; Arrighi, Silvana; Lacalandra, Giovanni M; Cremonesi, Fausto

    2009-01-01

    Background The identification of the adipocyte-derived obesity gene product, leptin (Ob), and subsequently its association with reproduction in rodents and humans led to speculations that leptin may be involved in the regulation of oocyte and preimplantation embryo development. In mice and pigs, in vitro leptin addition significantly increased meiotic resumption and promoted preimplantation embryo development in a dose-dependent manner. This study was conducted to determine whether leptin supplementation during in vitro maturation (IVM) to horse oocytes could have effects on their developmental capacity after fertilization by IntraCytoplasmic Sperm Injection (ICSI). Methods Compact and expanded-cumulus horse oocytes were matured in medium containing different concentrations (1, 10, 100, 1000 ng/ml) of recombinant human leptin and the effects on maturation, fertilization and embryo cleavage were evaluated. Furthermore, early developmental expression of Ob and leptin receptor (Ob-R) was investigated by immunocytochemical staining. Results In expanded-cumulus oocytes, the addition of leptin in IVM medium improved maturation (74% vs 44%, for 100 ng/ml leptin-treated and control groups, respectively; P < 0.05) and fertilization after ICSI (56% vs 23% for 10 ng/ml leptin-treated and control groups, respectively; P < 0.05). However, the developmental rate and quality of 8-cell stage embryos derived from leptin-treated oocytes (100 ng/ml) was significantly reduced, in contrast to previous data in other species where leptin increased embryo cleavage. Ob and Ob-R proteins were detected up to the 8-cell stage with cortical and cytoplasmic granule-like distribution pattern in each blastomere. Conclusion Leptin plays a cumulus cell-mediated role in the regulation of oocyte maturation in the mare. Species-specific differences may exist in oocyte sensitivity to leptin. PMID:19835605

  11. Work and Family Research in IO/OB: Content Analysis and Review of the Literature (1980-2002)

    ERIC Educational Resources Information Center

    Eby, Lillian T.; Casper, Wendy J.; Lockwood, Angie; Bordeaux, Chris; Brinley, Andi

    2005-01-01

    This monograph reviews 190 work-family studies published in IO/OB journals from 1980 to 2002. The results of a content analysis are presented which catalog these articles with respect to the study focus, nature and direction of the proposed effects, and predictor, criterion, and mediator variables examined. Then a narrative review of the articles…

  12. Optimizing OBS data using shielding and by removing ocean wave loading noise with Pressure and Horizontal Pressure Gradient Sensor Data

    NASA Astrophysics Data System (ADS)

    Webb, Spahr C.; Barclay, Andrew H.

    2016-04-01

    Ocean bottom seismometer (OBS) data, particularly from sites in shallow water are notoriously noisy. Ocean currents generate forces on an unshielded OBS sensor causing time varying tilt that greatly raises noise levels. Shallow burial can mitigate this source of noise, but is expensive and difficult to accomplish, particularly for large fleets of instruments. Large shields can provide significant reduction in noise levels as demonstrated with Cascadia Array OBS data. A recent test deployment investigated the relative motion of the shield and the sensor within the shield, providing guidance on the effectiveness of shielding. Even with shielding or burial, deformation of the seafloor caused by loading by the ocean waves raises seismic noise levels. We have previously shown the vertical component noise can be reduced using data from pressure sensors to predict the vertical deformation and remove it from the vertical record. We recently deployed a new OBS instrument in shallow water (80m) that measured two horizontal components of pressure gradient and show these data can be used to reduce wave induced deformation noise from horizontal component seismic data.

  13. Methanol suppression of trichloroethylene degradation by Methylosinus trichosporium (OB3b) and methane-oxidizing mixed cultures

    SciTech Connect

    Eng, W.; Palumbo, A.V.; Strandberg, G.W.; Sriharan, S.

    1991-12-31

    The effect of methanol on trichloroethylene (TCE) degradation by mixed and pure methylotrophic cultures was examined in batch culture experiments. Methanol was found to relieve growth inhibition of Methylosinus trichosporium (OB3b) at high (14 mg/L) TCE concentrations. Degradation of TCE was determined by both radiolabeling and gas chromatography techniques. When cultures were grown on methanol over 10 to 14 d with 0.3 mg/L TCE, OB3b degraded 16.89 {+-} 0.82% (mean {+-} SD) of the TCE, and a mixed culture (DT type II) degraded 4.55 {+-} 0.11%. Mixed culture (JS type I) degraded 4.34 {+-} 0.06% of the TCE. When grown on methane with 0.3 mg/L TCE, 32.93 {+-} 2.01% of the TCE was degraded by OB3b, whereas the JS culture degraded 24.3 {+-} 1.38% of the TCE, and the DT culture degraded 34.3 {+-} 2.97% of the TCE. The addition of methanol to cultures grown on methane reduced TCE degradation to 16.21 {+-} 1.17% for OB3b and to 5.08 {+-} 0.56% for JS. Although methanol reduces the toxicity of TCE to the cultures, biodegradation of TCE cannot be sustained in methanol-grown cultures. Since high TCE concentrations appear to inhibit methane uptake and growth, we suggest the primary toxicity of TCE is directed towards the methane monooxygenase.

  14. Controlling the onset of OB/OM in a semiconductor quantum well system in an inverted Y-type configuration

    NASA Astrophysics Data System (ADS)

    Raheli, Ali; Hamedi, H. R.; Sahrai, M.

    2016-01-01

    The problem of optical bistability (OB) and optical multistability (OM) is numerically investigated in a four-level inverted Y-type semiconductor quantum well (SQW) structure immersed in a unidirectional ring cavity. In the four-level SQW system under consideration, a closed loop configuration is coupled to the upper level through a tunable probe field. We show that the OB threshold intensity can be controlled via the intensity of coupling fields which gives rise to the absorption variation of the probe field. In addition, due to the existence of the closed-loop configuration, the OB and OM behaviors of the proposed SQW medium are dependent on the relative phase of the applied fields. It is found that the OB can be switched to OM or vice versa by properly adjusting the relative phase of the applied fields. The results may provide new possibilities in real experiments for realizing an all-optical switching or coding element in a solid-state platform.

  15. The expression of ob gene is not acutely regulated by insulin and fasting in human abdominal subcutaneous adipose tissue.

    PubMed

    Vidal, H; Auboeuf, D; De Vos, P; Staels, B; Riou, J P; Auwerx, J; Laville, M

    1996-07-15

    The regulation of ob gene expression in abdominal subcutaneous adipose tissue was investigated using a reverse transcription-competitive PCR method to quantify the mRNA level of leptin. Leptin mRNA level was highly correlated with the body mass index of 26 subjects (12 lean, 7 non-insulin-dependent diabetic, and 7 obese patients). The effect of fasting on ob gene expression was investigated in 10 subjects maintained on a hypocaloric diet (1045 KJ/d) for 5 d. While their metabolic parameters significantly changed (decrease in insulinemia, glycemia, and resting metabolic rate and increase in plasma ketone bodies), the caloric restriction did not modify the leptin mRNA level in the adipose tissue. To verify whether insulin regulates ob gene expression, six lean subjects underwent a 3-h euglycemic hyperinsulinemic (846 +/- 138 pmol/liter) clamp. Leptin and Glut 4 mRNA levels were quantified in adipose tissue biopsies taken before and at the end of the clamp. Insulin infusion produced a significant threefold increase in Glut 4 mRNA while leptin mRNA was not affected. It is concluded that ob gene expression is not acutely regulated by insulin or by metabolic factors related to fasting in human abdominal subcutaneous adipose tissue. PMID:8755631

  16. Using Ambient Noise Data from the ALBACORE OBS Array to Determine a 3D Seismic Velocity Model Offshore Southern California

    NASA Astrophysics Data System (ADS)

    Kohler, M. D.; Bowden, D. C.; Tsai, V. C.; Weeraratne, D. S.

    2014-12-01

    The Pacific-North America plate boundary in Southern California extends far west of the coastline, and a 12-month ocean bottom seismometer (OBS) array spanned the western side of the plate boundary in order to image seismic velocities in the lithosphere. Velocities are modeled through stacked cross correlations of ambient noise data. The offshore data come primarily from the OBS array that collected 12 months of continuous data during 2010-2011, combined with Southern California Seismic Network (SCSN) station data. The cross correlations were stacked for noise correlation functions and examined using standard time- and frequency-domain methods to determine phase velocity and group velocity dispersion curves. Signals between the vertical-component OBS and co-located horizontal-component OBS observations associated with tilt noise, and pressure gauge observations associated with infragravity waves, were examined to further improve signals. The non-elastic noise was estimated by calculating the transfer functions between the vertical-to-horizontal and vertical-to-pressure components, and subtracting the coherent signal between the two from the vertical-component time series. We find that these effects are small in our dataset. We are simultaneously inverting all measureable dispersion curves to solve for 3D crustal velocity structure. Shear-wave velocities comprise the direct solution, and Vp/Vs ratios are constrained as much as the data allow. Calculations on data from 780 OBS-OBS, SCSN-SCSN, and OBS-SCSN pairs filtered around multiple narrow bands between 5 and 50 s show clear propagating waves traveling at group velocities between 1.2 and 3.5 km/s. The longer-term outcome of this work will comprise a 3D crustal and uppermost mantle velocity model with areal coverage not attainable before the deployment of the ocean bottom seismometers. The results define the transition in three dimensions from continental lithospheric structure in the near-shore region to oceanic

  17. Waveform Inversion of OBS Data and Illumination/Resolution Analyses on Marine Seismic Data Acquisitions by the Adjoint Wavefield Method

    NASA Astrophysics Data System (ADS)

    Chen, H.; Li, K.

    2012-12-01

    We applied a wave-equation based adjoint wavefield method for seismic illumination/resolution analyses and full waveform inversion. A two-way wave-equation is used to calculate directional and diffracted energy fluxes for waves propagating between sources and receivers to the subsurface target. The first-order staggered-grid pressure-velocity formulation, which lacks the characteristic of being self-adjoint is further validated and corrected to render the modeling operator before its practical application. Despite most published papers on synthetic kernel research, realistic applications to two field experiments are demonstrated and emphasize its practical needs. The Fréchet sensitivity kernels are used to quantify the target illumination conditions. For realistic illumination measurements and resolution analyses, two completely different survey geometries and nontrivial pre-conditioning strategies based on seismic data type are demonstrated and compared. From illumination studies, particle velocity responses are more sensitive to lateral velocity variations than pressure records. For waveform inversion, the more accurately estimated velocity model obtained the deeper the depth of investigation would be reached. To achieve better resolution and illumination, closely spaced OBS receiver interval is preferred. Based on the results, waveform inversion is applied for a gas hydrate site in Taiwan for shallow structure and BSR detection. Full waveform approach potentially provides better depth resolution than ray approach. The quantitative analyses, a by-product of full waveform inversion, are useful for quantifying seismic processing and depth migration strategies.llumination/resolution analysis for a 3D MCS/OBS survey in 2008. Analysis of OBS data shows that pressure (top), horizontal (middle) and vertical (bottom) velocity records produce different resolving power for gas hydrate exploration. ull waveform inversion of 8 OBS data along Yuan-An Ridge in SW Taiwan

  18. Searching for unconventional seismic signals on a subduction zone with a submerged forearc: OBS offshore the Lesser Antilles

    NASA Astrophysics Data System (ADS)

    Bécel, Anne; Diaz, Jordi; Laigle, Mireille; Hirn, Alfred

    2013-09-01

    Detecting unconventional seismic signals related to subduction zone processes at depth in continuous ocean bottom seismometer (OBS) records requires the analysis and identification of noise due to instrumental problems, deployment sites or sea state conditions. The temporary OBS deployment at the Lesser Antilles subduction zone provides new insights into the feasibility of detecting unconventional signals such as non volcanic tremor (NVT), long-period (LP) or ultra-long period (ULP) events. Analysis of noise at an array comprising several sites and types of instruments and comparison with recordings on land shows transients in the noise. Episodes can be identified considering the diversity of sites and instrument types and comparing the seismic signals with meteorological and oceanographic data. In order to reliably detect NVT (1-10 Hz) originating from inside the solid Earth, one must first characterize noise induced by the activity of the atmosphere and hydrosphere at the sea-bottom as well as on land. The semidiurnal modulation of noise amplitude can be shown here not to be due to that of the NVT from a seismic source at depth which is related to the subduction interplate and whose activity is modulated by the tidal stresses as inferred for other megathrusts on emerged forearcs. Here, the semidiurnal modulation is rather due to the effect of the tides themselves, such as tidal currents, since they do not affect all types and all components of the unique multi-station array of OBS that could be deployed on this submerged forearc. The short period cut-off of the strong noise due to ocean surface infragravity waves increases to longer periods with OBS depth, thereby increasing the observational window with low noise to lower frequencies, and deep OBS sites may be advantageous for detecting LP events.

  19. Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

    PubMed Central

    Farhan Ul-Haque, Muhammad; Kalidass, Bhagyalakshmi; Vorobev, Alexey; Baral, Bipin S.; DiSpirito, Alan A.

    2015-01-01

    Methanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. When Methylosinus trichosporium OB3b was grown in the presence of 1 μM CuCl2, expression of mmoX, encoding a subunit of the hydroxylase component of sMMO, was very low. mmoX expression increased, however, when methanobactin from Methylocystis sp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated with M. trichosporium OB3b. If M. trichosporium OB3b was grown in the absence of CuCl2, the mmoX expression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure of Methylosinus trichosporium OB3b to SB2-Mb had no effect on expression of mbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2. mbnA expression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin “piracy” may be commonplace. PMID:25616801

  20. Methanobactin from Methylocystis sp. strain SB2 affects gene expression and methane monooxygenase activity in Methylosinus trichosporium OB3b.

    PubMed

    Farhan Ul-Haque, Muhammad; Kalidass, Bhagyalakshmi; Vorobev, Alexey; Baral, Bipin S; DiSpirito, Alan A; Semrau, Jeremy D

    2015-04-01

    Methanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced by Methylosinus trichosporium OB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. When Methylosinus trichosporium OB3b was grown in the presence of 1 μM CuCl2, expression of mmoX, encoding a subunit of the hydroxylase component of sMMO, was very low. mmoX expression increased, however, when methanobactin from Methylocystis sp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated with M. trichosporium OB3b. If M. trichosporium OB3b was grown in the absence of CuCl2, the mmoX expression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure of Methylosinus trichosporium OB3b to SB2-Mb had no effect on expression of mbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2. mbnA expression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin "piracy" may be commonplace. PMID:25616801

  1. Fault Characterization in the Sea of Marmara (Turkey) Using OBS and Land Seismic Stations

    NASA Astrophysics Data System (ADS)

    Pinar, Ali; Yamamoto, Yojiro; Comoglu, Mustafa; Polat, Remzi; Turhan, Fatih; Takahashi, Narumi; Kalafat, Dogan; Citak, Seckin

    2016-04-01

    The fault segments of the North Anatolian fault (NAF) occurring between Tekirdag basin and Kumburgaz basin are investigated using 15 Ocean Bottom Seismic (OBS) stations. The OBS stations were deployed closely around the fault trace of NAF. During the observation period from September, 2014 until July, 2015 more than one thousand microearthquakes were determined. No uniform seismicity pattern was observed along strike and along dip of the fault segments in an area spanning 100 km from East to West of Marmara Sea. The western fault segments exhibit relatively higher and deeper seismic activity while the eastern segment show shallower and relatively lower seismic activity. Integrating the first motion polarity data from the land based stations of Kandilli Observatory and Earthquake Research Institute (KOERI) with the polarity data acquired from the OBS stations the focal mechanisms of 173 micro-earthquakes were determined. Most of the fault plane solutions indicate predominantly strike-slip mechanism. Several clusters of events are identified along the E-W extending NAF. We derive a focal mechanism for the individual events whenever the number of the polarities are sufficient. In addition, simultaneous inversion of the polarities in a cluster are done to retrieve a stress tensor along with focal mechanisms of the individual events in a cluster. A unique cluster of focal mechanisms was obtained from the events taking place in Western High (WH) region located between Tekirdag Basin (TB) and Central Basin (CB). Several features of this cluster are noticeable; 1) the site is the most seismically active part in Marmara Sea, 2) the site is the locus of the deepest events in the Sea of Marmara, 3) the shallower part of this segment is seismically less active, 4) two subgroups of P-axes of focal mechanisms exist; one oriented NW-SE and other oriented in N-S direction despite the proximity of the location of the events giving clues on the faulting dynamics. The N-S oriented P

  2. The 2.35 year itch of Cygnus OB2 #9. II. Radio monitoring

    NASA Astrophysics Data System (ADS)

    Blomme, R.; Nazé, Y.; Volpi, D.; De Becker, M.; Prinja, R. K.; Pittard, J. M.; Parkin, E. R.; Absil, O.

    2013-02-01

    Context. Cyg OB2 #9 is one of a small set of non-thermal radio emitting massive O-star binaries. The non-thermal radiation is due to synchrotron emission in the colliding-wind region. Cyg OB2 #9 has only recently been discovered to be a binary system, and a multi-wavelength campaign was organized to study its 2011 periastron passage. Aims: We want to better determine the parameters of this system and model the wind-wind collision. This will lead to a better understanding of the Fermi mechanism that accelerates electrons up to relativistic speeds in shocks and its occurrence in colliding-wind binaries. We report here on the results of the radio observations obtained in the monitoring campaign and present a simple model to interpret the data. Methods: We used the Expanded Very Large Array (EVLA) radio interferometer to obtain 6 cm and 20 cm continuum fluxes during the Cyg OB2 #9 periastron passage in 2011. We introduce a simple model to solve the radiative transfer in the stellar winds and the colliding-wind region, and thus determine the expected behaviour of the radio light curve. Results: The observed radio light curve shows a steep drop in flux sometime before periastron. The fluxes drop to a level that is comparable to the expected free-free emission from the stellar winds, suggesting that the non-thermal emitting region is completely hidden at that time. After periastron passage, the fluxes slowly increase. We use the asymmetry of the light curve to show that the primary has the stronger wind. This is somewhat unexpected if we use the astrophysical parameters based on theoretical calibrations. But it becomes entirely feasible if we take into account that a given spectral type-luminosity class combination covers a range of astrophysical parameters. The colliding-wind region also contributes to the free-free emission, which can help explain the high values of the spectral index seen after periastron passage. Combining our data with older Very Large Array (VLA

  3. A Cornucopia of Massive Binary Star Systems in the Cygnus OB2 Association: Fifty and Counting

    NASA Astrophysics Data System (ADS)

    Kobulnicky, Henry A.; Kiminki, D. C.; Burke, J. F.; Chapman, J. E.; Keller, E.; Lester, K. V.; Rolen, E.; Topel, E.; Lundquist, M. J.; Bhattacharjee, A.; Vargas Alvarez, C. A.; Runnoe, J. C.; Dale, D. A.

    2014-01-01

    Massive binary star systems produce nature's most energetic events, including some classes of supernovae, gamma-ray bursts, X-ray binaries, and double-degenerate objects that generate gravitational wave radiation. The Cygnus OB2 Association is the largest nearby collection of massive stars, consisting of several hundred O and early B stars at a distance of just 1.4 kpc. Our Cygnus OB2 Radial Velocity Survey team at the University of Wyoming has spectroscopically monitored 115 stars of type B2 or earlier between 1999 and 2013, accruing an average of 12 observations per star at a velocity precision of 2-6 km/s. We have identified fifty massive binary systems, nearly all of which have full orbital solutions. Periods range from 1.4 days - 12.5 years and velocity semi-amplitudes span 4-300 km/s. Monte-Carlo modeling indicates that as many as 90% of massive systems contain multiple stars and that 45% of these can be characterized as ``close'' binaries that will interact, exchanging matter during main-sequence or post-main-sequence evolution. Statistical analysis of the orbital parameters reveals a striking surplus of close, short-period systems with periods P=1.4--7 days, with fully 30% (17 out of 50 systems) of the known binaries falling in this tight range; their typical orbital separations are just a small fraction of an astronomical unit. The remainder of the binary systems are consistent with a period distribution described as flat in log(P) out to several thousand day periods. The mass ratio distribution appears flat over the interval q=M2/M1=0.1-1.0, meaning that massive stars preferentially have massive companions. These data constitute the largest and most complete homogeneous database on any single collection of massive stars in a common formation environment covering the full range of stars expected to explode as supernovae (B2V and earlier). As such, the Survey provides the raw data for modeling rates of cosmic supernova, gamma-ray bursts, and X-ray binaries

  4. The Escape of Ionizing Photons from OB Associations in Disk Galaxies: Radiation Transfer through Superbubbles

    NASA Astrophysics Data System (ADS)

    Dove, James B.; Shull, J. Michael; Ferrara, Andrea

    2000-03-01

    By solving the time-dependent radiation transfer problem of stellar radiation through evolving superbubbles within a smoothly varying H I distribution, we estimate the fraction of ionizing photons emitted by OB associations that escapes the H I disk of our Galaxy into the halo and intergalactic medium (IGM). We consider both coeval star formation and a Gaussian star formation history with a time spread σt=2 Myr. We consider both a uniform H I distribution and a two-phase (cloud/intercloud) model, with a negligible filling factor of hot gas. We find that the shells of the expanding superbubbles quickly trap or attenuate the ionizing flux, so that most of the escaping radiation escapes shortly after the formation of the superbubble. Superbubbles of large associations can blow out of the H I disk and form dynamic chimneys, which allow the ionizing radiation to escape the H I disk directly. However, blowout occurs when the ionizing photon luminosity has dropped well below the association's maximum luminosity. For the coeval star formation history, the total fraction of Lyman Continuum photons that escape both sides of the disk in the solar vicinity is ~0.15+/-0.05. For the Gaussian star formation history, ~0.06+/-0.03, a value roughly a factor of 2 lower than the results of Dove & Shull, where superbubbles were not considered. For a local production rate of ionizing photons ΨLyC=4.95x107 cm-2 s-1, the flux escaping the disk is ΦLyC~(1.5-3.0)x106 cm-2 s-1 for coeval and Gaussian star formation, comparable to the flux required to sustain the Reynolds layer. Rayleigh-Taylor instabilities exist early in the OB association's evolutionary stages, possibly causing the shell to fragment and increasing . However, if a significant fraction of H I is distributed in cold clouds with nH~30 cm-3, can be reduced by a factor of ~2-5 if the cloud properties are similar to ``standard clouds'' with a disk geometry.

  5. Seismic Velocity structures in Northern Izu-Bonin arc derived from passive OBS observations

    NASA Astrophysics Data System (ADS)

    Obana, K.; Kamiya, S.; Kodaira, S.; Suetsugu, D.; Takahashi, N.; Takahashi, T.; Tamura, Y.; Sakaguchi, H.

    2007-12-01

    The Izu-Bonin Island arc is an oceanic island arc, where the Pacific plate subducts beneath the Philippine Sea plate. Recent active seismic surveys in the Izu-Bonin arc show significant variations in thickness of the middle crust along the volcanic front [Kodaira et al, 2007]. To understand the crustal evolution in the oceanic island arc, we have to clarify structures in the mantle wedge along the arc in addition to the oceanic island arc crust. We conducted seismicity observations to investigate structure variations in northern Izu-Bonin arc using natural earthquakes. A temporal ocean bottom seismograph (OBS) network consists of 40 pop-up type OBSs was deployed in April 2006 between Tori-shima and Hachijo-jima islands. These OBSs were retrieved in July after about 80-day observations. We used continuous seismic data at 36 OBSs and three F-net and Hi-net seismic stations on Hachijo-jima and Aoga-shima islands operated by National Research Institute for Earth Science and Disaster Prevention. During the OBS observations, about 1600 earthquakes were located. These earthquakes clearly show double seismic zone along the subducting Pacific plate. We estimated 1D and 3D P- and S-wave seismic velocity structure using arrival time data of these earthquakes. The 1D velocity model shows that a layer with low Poisson's ratio of 0.24 and high Poisson's ratio of 0.28 corresponds to middle and lower crust, respectively. The low Poisson's ratio layer suggests the granitic middle crust with Vp of ~6km/s. The high Poisson's ratio layer agrees with the gabbroic lower crust as suggested by Kodaira et al. [2007]. Three-dimensional Vp and Vs structures were estimated by 3D tomographic inversion method by Kamiyra and Kobayashi [2000] using the 1D model as an initial model. The estimated 3D model shows structure variations along the volcanic front. We will discuss relationships between the seismic velocity variations and the island arc crust structures in northern Izu- Bonin arc.

  6. Identification of a novel agonist of peroxisome proliferator-activated receptors alpha and gamma that may contribute to the anti-diabetic activity of guggulipid in Lep(ob)/Lep(ob) mice.

    PubMed

    Cornick, Claire L; Strongitharm, Barbara H; Sassano, Gary; Rawlins, Christopher; Mayes, Andrew E; Joseph, Alison N; O'Dowd, Jacqueline; Stocker, Claire; Wargent, Ed; Cawthorne, Michael A; Brown, A Louise; Arch, Jonathan R S

    2009-10-01

    The ethyl acetate extract of the gum of the guggul tree, Commiphora mukul (guggulipid), is marketed for the treatment of dyslipidaemia and obesity. We have found that it protects Lep(ob)/Lep(ob) mice from diabetes and have investigated possible molecular mechanisms for its metabolic effects, in particular those due to a newly identified component, commipheric acid. Both guggulipid (EC(50)=0.82 microg/ml) and commipheric acid (EC(50)=0.26 microg/ml) activated human peroxisome proliferator-activated receptor alpha (PPARalpha) in COS-7 cells transiently transfected with the receptor and a reporter gene construct. Similarly, both guggulipid (EC(50)=2.3 microg/ml) and commipheric acid (EC(50)=0.3 microg/ml) activated PPARgamma and both promoted the differentiation of 3T3 L1 preadipocytes to adipocytes. Guggulipid (EC(50)=0.66 microg/ml), but not commipheric acid, activated liver X receptor alpha (LXRalpha). E- and Z-guggulsterones, which are largely responsible for guggulipid's hypocholesterolaemic effect, had no effects in these assays. Guggulipid (20 g/kg diet) improved glucose tolerance in female Lep(ob)/Lep(ob) mice. Pure commipheric acid, given orally (960 mg/kg body weight, once daily), increased liver weight but did not affect body weight or glucose tolerance. However, the ethyl ester of commipheric acid (150 mg/kg, twice daily) lowered fasting blood glucose and plasma insulin, and plasma triglycerides without affecting food intake or body weight. These results raise the possibility that guggulipid has anti-diabetic activity due partly to commipheric acid's PPARalpha/gamma agonism, but the systemic bioavailability of orally dosed, pure commipheric acid appears poor. Another component may contribute to guggulipid's anti-diabetic and hypocholesterolaemic activity by stimulating LXRalpha. PMID:18926687

  7. Central stars of planetary nebulae. II. New OB-type and emission-line stars

    NASA Astrophysics Data System (ADS)

    Weidmann, W. A.; Gamen, R.

    2011-07-01

    Context. There are more than 3000 confirmed and probably known Galactic planetary nebulae (PNe), but central star spectroscopic information is available for only 13% of them. Aims: We have undertaken a spectroscopic survey of the central stars in PNe to identify their spectral types. Methods: We performed spectroscopic observations at low resolution with the 2-m telescope at CASLEO, Argentina. Results: We present the spectra of 46 central stars of PNe, most of them are OB-type and emission-line stars. Based on data collected at the Complejo Astronómico El Leoncito (CASLEO), which is operated under agreement between the Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina y Universidades Nacionales de La Plata, Córdoba y San Juan, Argentina.The reduced spectra (FITS files) are available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/531/A172

  8. Intermediate scale structure of the interstellar medium towards NGC 6231 in Sco OB1 with FUSE

    NASA Astrophysics Data System (ADS)

    Marggraf, O.; Bluhm, H.; de Boer, K. S.

    2004-03-01

    The FUSE far-ultraviolet interstellar spectra toward seven targets in NGC 6231 show that the molecules H2, HD, and CO as well as various atomic species are distributed in several clouds. The main absorption component found on the sight lines lies in the Lupus cloud region at a distance of about 150 pc, and there is a weaker second one, presumably in the Sco OB1 shell surrounding NGC 6231 (d≃1.8 kpc). H2 excitation modelling is used to constrain the radiation field and the density in the gas; HD is used to estimate the abundance of H+. The small angular separation of the target stars allows column density variations to be probed over the field of view, on scales of 0.05 pc in the case of the Lupus cloud distance. They are 40% for H2 and 60% for H I. A rather strong radiation field inside the molecular clouds suggests a separation of the gas into smaller cloudlets also along the line of sight. Based on observations made with the NASA-CNES-CSA Far Ultraviolet Spectroscopic Explorer. FUSE is operated for NASA by the Johns Hopkins University under NASA contract NAS5-32985.

  9. Massive open star clusters using the VVV survey. V. Young clusters with an OB stellar population

    NASA Astrophysics Data System (ADS)

    Ramírez Alegría, S.; Borissova, J.; Chené, A.-N.; Bonatto, C.; Kurtev, R.; Amigo, P.; Kuhn, M.; Gromadzki, M.; Carballo-Bello, J. A.

    2016-04-01

    Context. The ESO public survey VISTA Variables in the Vía Láctea (VVV) has contributed with deep multi-epoch photometry of the Galactic bulge and the adjacent part of the disk over 526 sq. deg. More than a hundred cluster candidates have been reported thanks to this survey. Aims: We present the fifth article in a series of papers focused on young and massive clusters discovered in the VVV survey. In this paper, we present the physical characterization of five clusters with a spectroscopically confirmed OB-type stellar population. Methods: To characterize the clusters, we used near-infrared photometry (J, H, and KS) from the VVV survey and near-infrared K-band spectroscopy from ISAAC at VLT, following the methodology presented in the previous articles of the series. Results: All clusters in our sample are very young (ages between 1-20 Myr), and their total mass are between (1.07+0.40-0.30)×102 M⊙ and (4.17+4.15-2.08)×103 M⊙. We observed a relation between the clusters total mass Mecl and the mass of their most massive stellar member mmax, for clusters with an age <10 Myr. Based on observations taken within the ESO VISTA Public Survey VVV (programme ID 179.B-2002), and with ISAAC/VLT (programme 087.D-0341(A)).

  10. Spitzer IRS Spectra of Debris Disks in the Scorpius–Centaurus OB Association

    NASA Astrophysics Data System (ADS)

    Jang-Condell, Hannah; Chen, Christine H.; Mittal, Tushar; Manoj, P.; Watson, Dan; Lisse, Carey M.; Nesvold, Erika; Kuchner, Marc

    2015-08-01

    We analyze spectra obtained with the Spitzer Infrared Spectrograph (IRS) of 110 B-, A-, F-, and G-type stars with optically thin infrared excess in the Scorpius–Centaurus OB association. The ages of these stars range from 11 to 17 Myr. We fit the infrared excesses observed in these sources by Spitzer IRS and the Multiband Imaging Photometer for Spitzer (MIPS) to simple dust models according to Mie theory. We find that nearly all of the objects in our study can be fit by one or two belts of dust. Dust around lower mass stars appears to be closer in than around higher mass stars, particularly for the warm dust component in the two-belt systems, suggesting a mass-dependent evolution of debris disks around young stars. For those objects with stellar companions, all dust distances are consistent with truncation of the debris disk by the binary companion. The gaps between several of the two-belt systems can place limits on the planets that might lie between the belts, potentially constraining the mass and locations of planets that may be forming around these stars.

  11. DISK EVOLUTION IN OB ASSOCIATIONS: DEEP SPITZER/IRAC OBSERVATIONS OF IC 1795

    SciTech Connect

    Roccatagliata, Veronica; Bouwman, Jeroen; Henning, Thomas; Gennaro, Mario; Sicilia-Aguilar, Aurora; Feigelson, Eric; Kim, Jinyoung Serena; Lawson, Warrick A.

    2011-06-01

    We present a deep Spitzer/Infrared Array Camera (IRAC) survey of the OB association IC 1795 carried out to investigate the evolution of protoplanetary disks in regions of massive star formation. Combining Spitzer/IRAC data with Chandra/Advanced CCD Imaging Spectrometer observations, we find 289 cluster members. An additional 340 sources with an infrared excess, but without X-ray counterpart, are classified as cluster member candidates. Both surveys are complete down to stellar masses of about 1 M{sub sun}. We present pre-main-sequence isochrones computed for the first time in the Spitzer/IRAC colors. The age of the cluster, determined via the location of the Class III sources in the [3.6]-[4.5]/[3.6] color-magnitude diagram, is in the range of 3-5 Myr. As theoretically expected, we do not find any systematic variation in the spatial distribution of disks within 0.6 pc of either O-type star in the association. However, the disk fraction in IC 1795 does depend on the stellar mass: sources with masses >2 M{sub sun} have a disk fraction of {approx}20%, while lower mass objects (2-0.8 M{sub sun}) have a disk fraction of {approx}50%. This implies that disks around massive stars have a shorter dissipation timescale.

  12. La Asociación OB Bochum7 combinando datos IR y ópticos

    NASA Astrophysics Data System (ADS)

    Corti, M. A.; Bosch, G. L.; Niemela, V. S.

    We present the results of an analysis of IR data in the region of the galactic OB association Bo7, obtained from the archives of the IRAS satellite mission and the 2MASS survey. Bo7 is located at the end of Perseus spiral arm. Distances of possible members of the Bo7 association were determined calculating the absorption from the E(V-K) colour excess. These members had been previously selected according to their UBV colours and spectra. The distance values obtained with IR excess have a smaller error than those obtained considering the E(B-V) excess. An extended interstellar dust cloud (detected in IRAS maps) is found to be probably associated with the members of Bo7. Two IRAS point sources observed in the region have characteristics of star formation sites. One of these point sources has been observed in CS(2-1) by Bronfman et al. (1996), who determined a value of (LSR) velocity of 44 km/s, close to the velocity of stars in Bo7 (Corti et al. 2003). A group of main sequence O - B0.5 stars appear near the location of the aforementioned IRAS point source, suggesting sequential star formation in the Bo7 region.

  13. VizieR Online Data Catalog: Optical spectrum of Cyg OB2 No. 12 (Klochkova+, 2004)

    NASA Astrophysics Data System (ADS)

    Klochkova, V. G.; Chentsov, E. L.

    2006-04-01

    We have obtained the first high spectral-resolution (R=15000 and 60000) optical spectra for the extremely luminous star No.12, identified with the IR-source IRAS 20308+4104 in the Cyg OB2 association. We have identified about 200 spectral features at 4552-7939{AA}, including interstellar NaI, KI and numerous DIBs, which are in the strongest absorptions in the spectrum, along with the HeI, CII, SiII lines. A two-dimensional spectral classification indicates that the star's spectral type is B5+/-0.5 Ia+. Our analyses of the radial velocities pattern shows the presence of a radial velocity gradient in the stellar atmosphere due to the infall of matter onto the star. The strong H(alpha) emission line displays broad Thompson wings and time-variable core absorption, providing evidence that the stellar wind is inhomogeneous and slightly blue-shifted P Cygni absorption profile. We conclude that the wind is time-variable. (1 data file).

  14. Active and passive interferometry with a deep-water OBS array

    NASA Astrophysics Data System (ADS)

    Carrière, O.; Gerstoft, P.

    2012-12-01

    Green's function estimation between two points from ambient noise relies on the assumption of diffuse noise field. Directional noise field from ships or other anthropogenic activity may bias the travel time estimates. In April 2011, fifteen ocean bottom seismometers (OBS) were deployed on a straight line in the Gulf of Mexico at 900-m water depth. During the next few days, both ambient noise data and active surveys using air-gun were recorded. This work compares the reflectivity estimates obtained from controlled source (active) interferometry and ambient noise (passive) crosscorrelation of hydrophone and geophone signals. Compared to conventional seismic processing, active interferometry suppresses the need of survey source position knowledge and removes statics. Passive interferometry removes the need of active surveys but typically requires longer term deployment. The noise sequence collected in the Gulf of Mexico is dominated by a distant seismic exploration from a single direction. The impact of such strong and highly directional and acoustic source on the Green's function extraction is discussed and compared to simulated diffuse noise field crosscorrelation. The utility of beamforming technique and eigenvector subspace projection for attenuating the directional noise is examined in details.

  15. Angular Momentum Evolution of Pre-Main Sequence Stars in the Nearest OB Association

    NASA Astrophysics Data System (ADS)

    Oberst, Thomas E.; Mellon, Samuel N.; Pecaut, Mark J.; Mamajek, Eric E.

    2015-08-01

    We present an analysis of the angular momentum evolution of pre-main sequence stars in the Scorpius-Centaurus (Sco-Cen) OB association, the nearest to the Sun. Our parent sample consists of 1692 Sco-Cen members, 157 of which are previously unpublished. Using archival SuperWASP photometry, we detect starspot modulation over multiple seasons for 183 of these stars and estimate their rotation periods. Spectral types have been measured for 24 of these stars using low-resolution spectra from the SMARTS 1.5 m telescope, with the remainder adopted from the literature. Our stellar sample spans spectral types from late-A to mid-M dwarfs with periods from ~ 0.2 - 8 days and isochronal ages from ~ 11 - 17 Myr. We examine the relationship between mass, period, and age to constrain models of angular momentum evolution, furthering our understanding of stars which have completed their main accretion phase (~ 1 - 10 Myr) but have not yet reached the zero-age main sequence (~ 40 Myr for 1 M⊙).

  16. Antidiabetic thiazolidinediones inhibit leptin (ob) gene expression in 3T3-L1 adipocytes.

    PubMed Central

    Kallen, C B; Lazar, M A

    1996-01-01

    Lack of leptin (ob) protein causes obesity in mice. The leptin gene product is important for normal regulation of appetite and metabolic rate and is produced exclusively by adipocytes. Leptin mRNA was induced during the adipose conversion of 3T3-L1 cells, which are useful for studying adipocyte differentiation and function under controlled conditions. We studied leptin regulation by antidiabetic thiazolidinedione compounds, which are ligands for the adipocyte-specific nuclear receptor peroxisome proliferator-activated receptor gamma (PPARgamma) that regulates the transcription of other adipocyte-specific genes. Remarkably, leptin gene expression was dramatically repressed within a few hours after thiazolidinedione treatment. The ED50 for inhibition of leptin expression by the thiazolidinedione BRL49653 was between 5 and 50 nM, similar to its Kd for binding to PPARgamma. The relatively weak, nonthiazolidinedione PPAR activator WY 14,643 also inhibited leptin expression, but was approximately 1000 times less potent than BRL49653. These results indicate that antidiabetic thiazolidinediones down-regulate leptin gene expression with potencies that correlate with their abilities to bind and activate PPARgamma. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 PMID:8650171

  17. First OB-stars in the iron-poor local group galaxy Sextans A

    NASA Astrophysics Data System (ADS)

    Camacho, I.; García, M.; Herrero, A.

    2015-05-01

    Massive stars are crucial to understand the chemical and dynamical evolution of the Universe. Our knowledge of their physics at sub-SMC metallicities, dominant in the early Universe, is still in its infancy, due to the lack of proper observations and analyses. We have started a programme to observe stars in nearby galaxies with metallicities below that of the SMC. For this purpose, we have developed photometric criteria which allowed us to set a list of blue massive candidates in Sextans A, a galaxy with a metallicity of [Fe/H]= -1.85. We have secured OSIRIS@GTC spectra of these candidates and have discovered six O-type and seven early-B stars, which constitutes the first atlas of blue massive stars in this galaxy. Using FASTWIND model atmospheres, we have obtained the main physical parameters of the O-type stars (effective temperature, gravity, wind strength and He content).This is the lowest metallicity for which an observational temperature scale of O-stars has been stablished. This work is part of the PhD of I. Camacho aimed at the identification, classification and analysis of massive OB stars in low metallicity galaxies.

  18. VizieR Online Data Catalog: Orion Trapezium area ROSAT PSPC obs. I. (Geier+, 1995)

    NASA Astrophysics Data System (ADS)

    Geier, S.; Wendker, H. J.; Wisotzki, L.

    2000-10-01

    A deep ROSAT PSPC image centred on the Orion Trapezium has revealed that most of the X-ray emission originates from discrete sources, in contrast to previous EINSTEIN data which suggested a diffuse emission component. We present a list of 171 X-ray sources all situated in the so-called inner ring of the field of view (20' radius). The field is crowded with sources. A special procedure had to be developed to cope with the severe blending of sources. The present list is not complete for LX<3.5*1029erg/s due to this reason and many more sources can still be expected by a next step in the reduction. Nearly all of the sources could be identified with pre-main sequence stars of the Ori OB 1 association in its subgroups Ic and Id. The statistics of this ensemble are discussed. It seems that members of the above subgroups can be distinguished on the basis of an additional amount of X-ray extinction seen in their spectra or hardness ratios. The O stars in the area ({teta}1 Ori C, {teta}2 Ori A and {iota} Ori) are briefly discussed. Six B stars are identified with X-ray sources. (2 data files).

  19. THE GALACTIC SPATIAL DISTRIBUTION OF OB ASSOCIATIONS AND THEIR SURROUNDING SUPERNOVA-GENERATED SUPERBUBBLES

    SciTech Connect

    Higdon, J. C.; Lingenfelter, R. E. E-mail: rlingenfelter@ucsd.edu

    2013-10-01

    The Galactic spatial distribution of OB associations and their surrounding superbubbles (SBs) reflect the distribution of a wide range of important processes in our Galaxy. In particular, it can provide a three-dimensional measure not only of the major source distribution of Galactic cosmic rays, but also the Galactic star formation distribution, the Lyman continuum ionizing radiation distribution, the core-collapse supernova distribution, the neutron star and stellar black hole production distribution, and the principal source distribution of freshly synthesized elements. Thus, we construct a three-dimensional spatial model of the massive-star distribution based primarily on the emission of the H II envelopes that surround the giant SBs and are maintained by the ionizing radiation of the embedded O stars. The Galactic longitudinal distribution of the 205 μm N II radiation, emitted by these H II envelopes, is used to infer the spatial distribution of SBs. We find that the Galactic SB distribution is dominated by the contribution of massive-star clusters residing in the spiral arms.

  20. VizieR Online Data Catalog: Northern Galactic OB stars vsini (Simon-Diaz+, 2014)

    NASA Astrophysics Data System (ADS)

    Simon-Diaz, S.; Herrero, A.

    2014-04-01

    The spectroscopic observations considered for this study are part of the IACOB spectroscopic database of northern Galactic OB stars (last described in Simon-Diaz et al., 2011, Bull. Soc. Roy. Sci. Liege, 80, 514 and Stellar Clusters and Associations: Proc. A RIA Workshop on Gaia, eds. E. J. Alfaro Navarro, A. T. Gallego Calvente, & M. R. Zapatero Osorio, 255). This unique high-quality spectroscopic database has been compiled in the framework of the IACOB project. To date, the IACOB database comprises 1250 spectra of 153 and 97 Galactic O- and early B-type stars, respectively, observable from the Roque de los Muchachos observatory in La Palma (Spain). The spectra have a resolving power of 46000 and 23000, a typical signal-to-noise ratio (S/N) above 150, and were compiled between November 2008 and January 2013 with the high-resolution FIbre-fed Echelle Spectrograph (FIES) attached to the Nordic Optical Telescope (NOT). The IACOB database has a multi-epoch character that enables investigations of the binary/multiple nature of considered stars and the temporal variations in individual objects with at least three spectra per observed target. In this study, we only used a subsample of the spectra, discarding all stars with signatures of multiplicity (which means that we only considered apparently single and SB1 stars), and only considering the spectrum with the highest S/N ratio per star. (5 data files).

  1. An ALMA continuum survey of circumstellar disks in the upper Scorpius OB association

    SciTech Connect

    Carpenter, John M.; Ricci, Luca; Isella, Andrea

    2014-05-20

    We present ALMA 880 μm continuum observations of 20 K- and M-type stars in the Upper Scorpius OB association (Upper Sco) that are surrounded by protoplanetary disks. These data are used to measure the dust content in disks around low-mass stars (0.1-1.6 M {sub ☉}) at a stellar age of 5-11 Myr. Thirteen sources were detected in the 880 μm dust continuum at ≥3σ with inferred dust masses between 0.3 and 52 M {sub ⊕}. The dust masses tend to be higher around the more massive stars, but the significance is marginal in that the probability of no correlation is p ≈ 0.03. The evolution in the dust content in disks was assessed by comparing the Upper Sco observations with published continuum measurements of disks around ∼1-2 Myr stars in the Class II stage in the Taurus molecular cloud. While the dust masses in the Upper Sco disks are on average lower than in Taurus, any difference in the dust mass distributions is significant at less than 3σ. For stellar masses between 0.49 M {sub ☉} and 1.6 M {sub ☉}, the mean dust mass in disks is lower in Upper Sco relative to Taurus by Δlog M {sub dust} = 0.44 ± 0.26.

  2. Isolation and Purification of Cu-free Methanobactin from Methylosinus trichosporium OB3b

    SciTech Connect

    M Pesch; I Christl; K Barmettler; S Kraemer; R Kretzschmar

    2011-12-31

    The isolation of highly pure copper-free methanobactin is a prerequisite for the investigation of the biogeochemical functions of this chalkophore molecule produced by methane oxidizing bacteria. Here, we report a purification method for methanobactin from Methylosinus trichosporium OB3b cultures based on reversed-phase HPLC fractionation used in combination with a previously reported resin extraction. HPLC eluent fractions of the resin extracted product were collected and characterized with UV-vis, FT-IR, and C-1s NEXAFS spectroscopy, as well as with elemental analysis and ESI-MS. The results showed that numerous compounds other than methanobactin were present in the isolate obtained with resin extraction. Molar C/N ratios, mass spectrometry measurements, and UV-vis spectra indicated that methanobactin was only present in one of the HPLC fractions. On a mass basis, methanobactin carbon contributed only 32% to the total organic carbon isolated with resin extraction. Our spectroscopic results implied that besides methanobactin, the organic compounds in the resin extract comprised breakdown products of methanobactin as well as polysaccharide-like substances. Our results demonstrate that a purification step is indispensable in addition to resin extraction in order to obtain pure methanobactin. The proposed HPLC purification procedure is suitable for semi-preparative work and provides copper-free methanobactin.

  3. No Evidence for Protoplanetary Disk Destruction By OBS Stars in the MYStIX Sample

    NASA Astrophysics Data System (ADS)

    Richert, Alexander J. W.; Feigelson, Eric D.; Getman, Konstantin V.; Kuhn, Michael A.

    2015-09-01

    Hubble Space Telescope images of proplyds in the Orion Nebula, as well as submillimeter/radio measurements, show that the dominant O7 star {θ }1Ori C photoevaporates nearby disks around pre-main-sequence stars. Theory predicts that massive stars photoevaporate disks within distances of the order of 0.1 pc. These findings suggest that young, OB-dominated massive H ii regions are inhospitable to the survival of protoplanetary disks and, subsequently, to the formation and evolution of planets. In the current work, we test this hypothesis using large samples of pre-main-sequence stars in 20 massive star-forming regions selected with X-ray and infrared photometry in the MYStIX survey. Complete disk destruction would lead to a deficit of cluster members with an excess in JHKS and Spitzer/IRAC bands in the vicinity of O stars. In four MYStIX regions containing O stars and a sufficient surface density of disk-bearing sources to reliably test for spatial avoidance, we find no evidence for the depletion of inner disks around pre-main-sequence stars in the vicinity of O-type stars, even very luminous O2–O5 stars. These results suggest that massive star-forming regions are not very hostile to the survival of protoplanetary disks and, presumably, to the formation of planets.

  4. BOREAS TE-1 CO2 and CH4 Flux Data Over the SSA-OBS Site

    NASA Technical Reports Server (NTRS)

    Anderson, Darwin; Papagno, Andrea; Hall, Forrest G. (Editor); Newcomer, Jeffrey A. (Editor)

    2000-01-01

    The BOREAS TE-1 team collected various data to characterize the soil-plant systems in the BOREAS SSA. Particular emphasis was placed on nutrient biochemistry, the stores and transfers of organic carbon, and how the characteristics were related to measured methane fluxes. The overall transect in the Prince Albert National Park (Saskatchewan, Canada) included the major plant communities and related soils that occurred in that section of the boreal forest. Soil physical, chemical, and biological measurements along the transect were used to characterize the static environment, which allowed them to be related to methane fluxes. Chamber techniques were used to provide a measure of methane production/uptake. Chamber measurements coupled with flask sampling were used to determine the seasonality of methane fluxes. This particular data set contains carbon dioxide and methane flux values from the SSA-OBS site. The data were collected from 09-Jun to 04-Sep-1994. The data are stored in tabular ASCII files. The data files are available on a CD-ROM (see document number 20010000884), or from the Oak Ridge National Laboratory (ORNL) Distributed Active Archive Center (DAAC).

  5. New Version of SeismicHandler (SHX) based on ObsPy

    NASA Astrophysics Data System (ADS)

    Stammler, Klaus; Walther, Marcus

    2016-04-01

    The command line version of SeismicHandler (SH), a scientific analysis tool for seismic waveform data developed around 1990, has been redesigned in the recent years, based on a project funded by the Deutsche Forschungsgemeinschaft (DFG). The aim was to address new data access techniques, simplified metadata handling and a modularized software design. As a result the program was rewritten in Python in its main parts, taking advantage of simplicity of this script language and its variety of well developed software libraries, including ObsPy. SHX provides an easy access to waveforms and metadata via arclink and FDSN webservice protocols, also access to event catalogs is implemented. With single commands whole networks or stations within a certain area may be read in, the metadata are retrieved from the servers and stored in a local database. For data processing the large set of SH commands is available, as well as the SH scripting language. Via this SH language scripts or additional Python modules the command set of SHX is easily extendable. The program is open source, tested on Linux operating systems, documentation and download is found at URL "https://www.seismic-handler.org/".

  6. Kinetics of chlorinated hydrocarbon degradation by Methylosinus trichosporium OB3b and toxicity of trichloroethylene.

    PubMed Central

    Oldenhuis, R; Oedzes, J Y; van der Waarde, J J; Janssen, D B

    1991-01-01

    The kinetics of the degradation of trichloroethylene (TCE) and seven other chlorinated aliphatic hydrocarbons by Methylosinus trichosporium OB3b were studied. All experiments were performed with cells grown under copper stress and thus expressing soluble methane monooxygenase. Compounds that were readily degraded included chloroform, trans-1,2-dichloroethylene, and TCE, with Vmax values of 550, 330, and 290 nmol min-1 mg of cells-1, respectively. 1,1-Dichloroethylene was a very poor substrate. TCE was found to be toxic for the cells, and this phenomenon was studied in detail. Addition of activated carbon decreased the acute toxicity of high levels of TCE by adsorption, and slow desorption enabled the cells to partially degrade TCE. TCE was also toxic by inactivating the cells during its conversion. The degree of inactivation was proportional to the amount of TCE degraded; maximum degradation occurred at a concentration of 2 mumol of TCE mg of cells-1. During conversion of [14C]TCE, various proteins became radiolabeled, including the alpha-subunit of the hydroxylase component of soluble methane monooxygenase. This indicated that TCE-mediated inactivation of cells was caused by nonspecific covalent binding of degradation products to cellular proteins. Images PMID:2036023

  7. Leptin (ob gene) of the South African clawed frog Xenopus laevis

    PubMed Central

    Crespi, Erica J.; Denver, Robert J.

    2006-01-01

    Leptin, the protein product of the obese (ob) gene, is a type-I cytokine hormone secreted by fat that is integral to food intake regulation and influences almost every physiological system in juvenile and adult mammals. Since the identification of leptin in the mouse in 1994, biologists have searched for orthologous genes in other species with limited success. In this article, we report the identification and functional characterization of leptin and leptin receptor (LR) in Xenopus. Despite low amino acid sequence similarity to mammalian leptins (≈35%) the frog protein has a nearly identical predicted tertiary structure and can activate the frog and mouse LRs in vitro. We showed that recombinant frog leptin (rxLeptin) is a potent anorexigen in frogs, as it is in mammals, but this response does not develop until midprometamorphosis. However, during early prometamorphosis, exogenous rxLeptin induced growth and development of the hind limb, where LR mRNA is expressed. The rxLeptin also stimulated cell proliferation in cultured hind limbs from early prometamorphic tadpoles, as measured by [3H]thymidine uptake. These findings are evidence that leptin can influence limb growth and differentiation during early development. Furthermore, the isolation and characterization of leptin and its receptor in a nonamniote provides an essential foundation for elucidating the structural and functional evolution of this important hormone. PMID:16782821

  8. The structure of KPN03535 (gi|152972051), a novel putative lipoprotein from Klebsiella pneumoniae, reveals an OB-fold

    PubMed Central

    Das, Debanu; Kozbial, Piotr; Han, Gye Won; Carlton, Dennis; Jaroszewski, Lukasz; Abdubek, Polat; Astakhova, Tamara; Axelrod, Herbert L.; Bakolitsa, Constantina; Chen, Connie; Chiu, Hsiu-Ju; Chiu, Michelle; Clayton, Thomas; Deller, Marc C.; Duan, Lian; Ellrott, Kyle; Elsliger, Marc-André; Ernst, Dustin; Farr, Carol L.; Feuerhelm, Julie; Grzechnik, Anna; Grant, Joanna C.; Jin, Kevin K.; Johnson, Hope A.; Klock, Heath E.; Knuth, Mark W.; Krishna, S. Sri; Kumar, Abhinav; Marciano, David; McMullan, Daniel; Miller, Mitchell D.; Morse, Andrew T.; Nigoghossian, Edward; Nopakun, Amanda; Okach, Linda; Oommachen, Silvya; Paulsen, Jessica; Puckett, Christina; Reyes, Ron; Rife, Christopher L.; Sefcovic, Natasha; Tien, Henry J.; Trame, Christine B.; van den Bedem, Henry; Weekes, Dana; Wooten, Tiffany; Xu, Qingping; Hodgson, Keith O.; Wooley, John; Deacon, Ashley M.; Godzik, Adam; Lesley, Scott A.; Wilson, Ian A.

    2010-01-01

    KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA. This structure provides the foundation for further investigations into this divergent member of the OB-fold family. PMID:20944219

  9. Suspended-sediment and fresh-water discharges in the Ob and Yenisey rivers, 1960-1988

    NASA Astrophysics Data System (ADS)

    Meade, R. H.; Bobrovitskaya, N. N.; Babkin, V. I.

    2000-08-01

    Of the world's great rivers, the Ob and Yenisey rank among the largest suppliers of fresh water and among the smallest suppliers of suspended sediment to the coastal ocean. Sediment in the middle reaches of the rivers is mobilized from bordering terraces and exchanged between channels and flood plains. Sediment in the lower reaches of these great rivers is deposited and stored (permanently, on a millennial time scale) in flood plains. Sediment discharges, already small under natural conditions, are diminished further by large manmade reservoirs that trap significant proportions of the moving solids. The long winter freeze and sudden spring breakup impose a peakedness in seasonal water runoff and sediment discharge that contrasts markedly with that in rivers of the tropics and more temperate climates. Very little sediment from the Ob and Yenisey rivers is being transported to the open waters of the Arctic Ocean under present conditions.

  10. A 3D Seismic Velocity Model Offshore Southern California from Ambient Noise Tomography of the ALBACORE OBS Array

    NASA Astrophysics Data System (ADS)

    Kohler, M. D.; Bowden, D. C.; Tsai, V. C.; Weeraratne, D. S.

    2015-12-01

    The Pacific-North America plate boundary in Southern California extends far west of the coastline, and a 12-month ocean bottom seismometer (OBS) array spanned the western side of the plate boundary to image lithospheric seismic velocities. Velocities are modeled through stacked cross correlations of ambient noise data. Twelve months of continuous data were used from 22 OBS stations and ~30 coastal and island Southern California Seismic Network stations. Particular attention has been paid to improving signal-to-noise ratios in the noise correlations with OBS stations by removing the effects of instrument tilt and infragravity waves. Different applications of preprocessing techniques allow us to distinguish the fundamental and first higher order Rayleigh modes, especially in deep water OBS pairs where the water layer dominates crustal sensitivity of the fundamental mode. Standard time domain and frequency domain methods are used to examine surface wave dispersion curves for group and phase velocities between 5 and 50 second periods, and these are inverted for 3D velocity structure. The results define the transition in three dimensions from continental lithospheric structure in the near-shore region to oceanic structure west of the continental borderland. While the most prominent features of the model relate to thinning of the crust west of the Patton Escarpment, other notable anomalies are present north-to-south throughout the continental borderland and along the coast from the Los Angeles Basin to the Peninsular Ranges. The velocity model will help describe the region's tectonic history, as well as provide new constraints for determination of earthquake relocations and rupture styles.

  11. Effects of dopamine on leptin release and leptin gene (OB) expression in adipocytes from obese and hypertensive patients

    PubMed Central

    Alvarez-Aguilar, Cleto; Alvarez-Paredes, Alfonso Rafael; Lindholm, Bengt; Stenvinkel, Peter; García-López, Elvia; Mejía-Rodríguez, Oliva; López-Meza, Joel Edmundo; Amato, Dante; Paniagua, Ramon

    2013-01-01

    Background A reduction of dopaminergic (DAergic) activity with increased prolactin levels has been found in obese and hypertensive patients, suggesting its involvement as a pathophysiological mechanism promoting hypertension. Similarly, leptin action increasing sympathetic activity has been proposed to be involved in mechanisms of hypertension. The aim of this study was to analyze the effects of DA, norepinephrine (NE), and prolactin on leptin release and leptin gene (OB) expression in adipocytes from obese and hypertensive patients. Methods Leptin release and OB gene expression were analyzed in cultured adipocytes from 16 obese and hypertensive patients treated with DA (0.001, 0.01, 0.1, and 1.0 μmol/L), NE (1.0 μmol/L), insulin (0.1 μmol/L), and prolactin (1.0 μmol/L), and from five nonobese and normotensive controls treated with DA (1 μmol/L), NE (1 μmol/L), insulin (0.1 μmol/L), and prolactin (1.0 μmol/L). Results A dose-related reduction of leptin release and OB gene messenger ribonucleic acid expression under different doses of DA was observed in adipocytes from obese hypertensive patients. Whereas prolactin treatment elicited a significant increase of both leptin release and OB gene expression, NE reduced these parameters. Although similar effects of DA and NE were observed in adipocytes from controls, baseline values in controls were reduced to 20% of the value in adipocytes from obese hypertensive patients. Conclusion These results suggest that DAergic deficiency contributes to metabolic disorders linked to hyperleptinemia in obese and hypertensive patients. PMID:24348062

  12. Phytoplankton succession in the Ob-Yenisei Shallow zone of the Kara Sea based on Russian databases

    NASA Astrophysics Data System (ADS)

    Makarevich, P. R.; Larionov, V. V.; Moiseev, D. V.

    2015-07-01

    Here, data about the taxonomic composition and spatial distribution of planktonic microalgae in the Ob Bay and the southern Kara Sea in north Russia were analyzed during all hydrological seasons over 11 years (1996-2006). Data were obtained through detailed in situ observations. These data are part of our arctic phytoplankton database. Phytoplankton inhabiting the near shore continental area of the Kara Sea exhibited four phases in the annual succession cycle: a prevernal phase (cryoflora bloom), a vernal phase (ice-edge bloom), a summer-fall phase (mixed synthesis phase), and a winter phase (dormant phase). These phases were clearly differentiated based on the composition of dominant phytoplankton species complexes and quantitative characteristics (i.e., microalgal number and biomass). In the study region, which is completely covered by ice for most of the year (from October to June), the process of primary production begins at the same time as in ice-free coastal areas. Sub-ice blooming and growth of cryoflora initiate beneath the ice cover, long before it breaks down. In addition, from July to October, high phytoplankton biomass was recorded in Ob Bay and in areas adjacent to the Ob-Yenisei shallows. This information provides quantitative evidence for the higher productivity of waters off the Obestuary, compared to other coastal areas in the Kara Sea. The main factor responsible for this phenomenon is the permanent (during the warm season) transport of living and dead organic matter by river runoff to shelf waters.

  13. Receiver functions using OBS data: promises and limitations from numerical modelling and examples from the Cascadia Initiative

    NASA Astrophysics Data System (ADS)

    Audet, Pascal

    2016-06-01

    The expanding fleet of broad-band ocean-bottom seismograph (OBS) stations is facilitating the study of the structure and seismicity of oceanic plates at regional scales. For continental studies, an important tool to characterize continental crust and mantle structure is the analysis of teleseismic P receiver functions. In the oceans, however, receiver functions potentially suffer from several limiting factors that are unique to ocean sites and plate structures. In this study, we model receiver functions for a variety of oceanic lithospheric structures to investigate the possibilities and limitations of receiver functions using OBS data. Several potentially contaminating effects are examined, including pressure reverberations from the water column for various ocean-floor depths and the effects of a layer of low-velocity marine sediments. These modelling results indicate that receiver functions from OBS data are difficult to interpret in the presence of marine sediments, but shallow-water sites in subduction zone forearcs may be suitable for constraining various crustal elements around the locked megathrust fault. We propose using a complementary approach based on transfer function modelling combined with a grid search approach that bypasses receiver functions altogether and estimates model properties directly from minimally processed waveforms. Using real data examples from the Cascadia Initiative, we show how receiver and transfer functions can be used to infer seismic properties of the oceanic plate in both shallow (Cascadia forearc) and deep (Juan de Fuca Ridge) ocean settings.

  14. An anomalous extinction law in the Cep OB3b young cluster: Evidence for dust processing during gas dispersal

    SciTech Connect

    Allen, Thomas S.; Prchlik, Jakub J.; Megeath, S. Thomas; Gutermuth, Robert A.; Pipher, Judith L.; Naylor, Tim; Jeffries, R. D.

    2014-05-10

    We determine the extinction law through Cep OB3b, a young cluster of 3000 stars undergoing gas dispersal. The extinction is measured toward 76 background K giants identified with MMT/Hectospec spectra. Color excess ratios were determined toward each of the giants using V and R photometry from the literature, g, r, i, and z photometry from the Sloan Digital Sky Survey and J, H, and K{sub s} photometry from the Two Micron All Sky Survey. These color excess ratios were then used to construct the extinction law through the dusty material associated with Cep OB3b. The extinction law through Cep OB3b is intermediate between the R{sub V} = 3.1 and R{sub V} = 5 laws commonly used for the diffuse atomic interstellar medium and dense molecular clouds, respectively. The dependence of the extinction law on line-of-sight A{sub V} is investigated and we find the extinction law becomes shallower for regions with A{sub V} > 2.5 mag. We speculate that the intermediate dust law results from dust processing during the dispersal of the molecular cloud by the cluster.

  15. Triggering Risk Factors of the Burnout Syndrome in Ob/Gyn Physicians from a Reference Public University of Brazil

    PubMed Central

    Ferreira Bortoletti, Fátima; Teresa Benevides-Pereira, Ana Maria; Vasconcellos, Esdras Guerreiro; Siqueira, José Oliveira; Araujo Júnior, Edward; Nardozza, Luciano Marcondes Machado; Sebastiani, Ricardo Werner; Moron, Antonio Fernandes

    2012-01-01

    Objective. To identify the risk factors to the development of Burnout Syndrome in Ob/Gyn Brazilian physicians in four dimensions: emotional exhaustion (EE), professional repression (PR), dehumanization (De), and emotional distancing (EmD). Methods. A prospective cross-sectional study was realized with 48 Ob/Gyn physicians (12 lecturers, 12 attending physicians, 12 medical residents, and 12 graduate students) from Department of Obstetrics, São Paulo Federal University (UNIFESP). We used a sociodemographic questionnaire focusing on the activities (administrative, educational, healthcare, and research). We applied a Burnout Syndrome Inventory (BSI) composed of two parts: triggering factors (ISB1) and the Burnout Syndrome (ISB2). The ISB1 is composed of two scales: positive organizational conditions (POC) and negative organizational conditions (NOC). The ISB2 is composed of four scales: EE, PR, De, and EmD. Results. We observed a rate below and above average to POC and NOC, respectively. The dimensions recorded a level above average to EE, an index at the upper limit of the average to De, a median index to EmD, and a median index to PR. Conclusions. The Ob/Gyn physicians are in an area of vulnerability for the development of Burnout Syndrome due to the high level of EE and De, associated with a median index of PR. The high rate of NOC contributes to the triggering of this scenery. PMID:23304541

  16. Receiver functions using OBS data: Promises and limitations from numerical modelling and examples from the Cascadia Initiative

    NASA Astrophysics Data System (ADS)

    Audet, Pascal

    2016-03-01

    The expanding fleet of broadband ocean-bottom seismograph (OBS) stations is facilitating the study of the structure and seismicity of oceanic plates at regional scales. For continental studies, an important tool to characterize continental crust and mantle structure is the analysis of teleseismic P receiver functions. In the oceans, however, receiver functions potentially suffer from several limiting factors that are unique to ocean sites and plate structures. In this study we model receiver functions for a variety of oceanic lithospheric structures to investigate the possibilities and limitations of receiver functions using OBS data. Several potentially contaminating effects are examined, including pressure reverberations from the water column for various ocean-floor depths and the effects of a layer of low-velocity marine sediments. These modelling results indicate that receiver functions from OBS data are difficult to interpret in the presence of marine sediments, but shallow-water sites in subduction zone forearcs may be suitable for constraining various crustal elements around the locked megathrust fault. We propose using a complementary approach based on transfer function modelling combined with a grid search approach that bypasses receiver functions altogether and estimates model properties directly from minimally processed waveforms. Using real data examples from the Cascadia Initiative, we show how receiver and transfer functions can be used to infer seismic properties of the oceanic plate in both shallow (Cascadia forearc) and deep (Juan de Fuca Ridge) ocean settings.

  17. Comparison of AMSR-E and SSM/I snow parameter retrievals over the Ob river basin

    USGS Publications Warehouse

    Mognard, N.M.; Grippa, M.; LeToan, T.; Kelly, R.E.J.; Chang, A.T.C.; Josberger, E.G.

    2004-01-01

    Passive microwave observations from the Advanced Microwave Scanning Radiometer - EOS (AMSR-E) and from the Special Sensor Microwave Imager (SSM/I) are used to analyse the evolution of the snow pack in the Ob river basin during the snow season of 2002-03. The Ob river is the biggest Russian river with respect to its watershed area (2 975 000 km2). The Ob originates in the Altai mountains and flows northward across the vast West Siberian lowland towards the Arctic Ocean. The majority of snow cover is contained in the lowlands rather than in mountainous regions and persists for six months or more. During the snow season, surface air temperatures are very cold. Therefore, the combination of cold dry snow and large areas of uniform topography is ideal for snowpack extent and water equivalent retrievals from passive microwave observations. The thermal gradient through the snow pack is estimated and used to model the growth of the snow grain size and to compute the evolution of the passive microwave derived snow depth over the region. A comparison between the AMSR-E and SSM/I estimates is performed and the differences between the snow parameters from the two satellite instruments are analysed.

  18. Quantitative variation in obesity-related traits and insulin precursors linked to the OB gene region on human chromosome 7

    SciTech Connect

    Duggirala, R.; Stern, M.P.; Reinhart, L.J.

    1996-09-01

    Despite the evidence that human obesity has strong genetic determinants, efforts at identifying specific genes that influence human obesity have largely been unsuccessful. Using the sibship data obtained from 32 low-income Mexican American pedigrees ascertained on a type II diabetic proband and a multipoint variance-components method, we tested for linkage between various obesity-related traits plus associated metabolic traits and 15 markers on human chromosome 7. We found evidence for linkage between markers in the OB gene region and various traits, as follows: D7S514 and extremity skinfolds (LOD = 3.1), human carboxypeptidase A1 (HCPA1) and 32,33-split proinsulin level (LOD = 4.2), and HCPA1 and proinsulin level (LOD = 3.2). A putative susceptibility locus linked to the marker D7S514 explained 56% of the total phenotypic variation in extremity skinfolds. Variation at the HCPA1 locus explained 64% of phenotypic variation in proinsulin level and {approximately}73% of phenotypic variation in split proinsulin concentration, respectively. Weaker evidence for linkage to several other obesity-related traits (e.g., waist circumference, body-mass index, fat mass by bioimpedance, etc.) was observed for a genetic location, which is {approximately}15 cM telomeric to OB. In conclusion, our study reveals that the OB region plays a significant role in determining the phenotypic variation of both insulin precursors and obesity-related traits, at least in Mexican Americans. 66 refs., 3 figs., 4 tabs.

  19. Nonlinear waveform analysis for water-layer response and its application to high-frequency receiver function analysis using OBS array

    NASA Astrophysics Data System (ADS)

    Akuhara, Takeshi; Mochizuki, Kimihiro; Kawakatsu, Hitoshi; Takeuchi, Nozomu

    2016-07-01

    Determination of a response of the seawater column to teleseismic plane wave is important to suppress adverse effects of water reverberations in calculating receiver functions (RFs) using ocean-bottom seismometer (OBS) records. We present a novel nonlinear-waveform analysis method using the simulated annealing algorithm to determine such a water-layer response recorded by an OBS array. We then demonstrate its usefulness for the RF estimation through its application to synthetic and observed data. Synthetic experiments suggest that the water-layer response constrained in this way has a potential to improve RFs of OBS records drastically even in the high-frequency range (to 4 Hz). By applying it to data observed by the OBS array around the Kii Peninsula, southwestern Japan, we identified a low-velocity zone at the top of the subducting Philippine Sea plate. This zone may represent the incoming fluid-rich sediment layer that has been reported by active source seismic survey.

  20. They Might Be Giants: Confirming Candidate OB Stars While Netting a Large Sample of Massive Star Spectra in the Great Nebula in Carina

    NASA Astrophysics Data System (ADS)

    Povich, Matthew S.; McSwain, M. Virginia

    2013-02-01

    We propose one night of observations with the AAOmega instrument on the Anglo-Australian Telescope to obtain spectra of a large sample of massive stars in the Great Nebula in Carina, the nearest analog of extragalactic starburst regions. Our targets include >100 spectroscopically classified OB stars plus 55 candidate OB stars that we recently identified via X-ray emission and infrared (IR) spectral energy distributions (SEDs). These observations will confirm or reject individual candidate OB stars as massive members of the Carina Nebula stellar population, a vital test for our methodology that will pave the way to discovering new massive stars in other regions. Determining the nature of the candidate OB stars is critical to any census of the massive stellar population in Carina, impacting our understanding of the energetics and stellar initial mass function in this well-studied region. We will employ spectral modeling and broadband optical-IR SED fitting to derive physical properties (e.g. temperature, bolometric luminosity, surface gravity, and mass) of the known OB stars and those newly-confirmed candidate OB stars with high (ga100) signal-to- noise spectra.

  1. Erratum: ``Post-T Tauri Stars in the Nearest OB Association'' (AJ, 124, 1670 [2002])

    NASA Astrophysics Data System (ADS)

    Mamajek, Eric E.; Meyer, Michael R.; Liebert, James

    2006-04-01

    In Table 3 the F6 V-type spectral standard star HR 7061 is mistakenly aliased as HD 173677 but should be HD 173667. In §7.2 HIP 68282 is mistakenly aliased as ν1 Cen (``nu 1'') but should be υ1 Cen (``upsilon 1'') according to the Bright Star Catalog (D. Hoffleit & W. H. Warren, Jr. [New Haven: Yale Univ. Obs.; 1991]). The same error in SIMBAD has since been corrected. In Appendix A, third paragraph, the units of slope should be K [log(cm s-2)]-1, not K [log(cm s-1)]-1. In Appendix C there is an incorrect sign in the second term in equation (C6), and incorrect color ranges were published for equations (C6) and (C7). Equations (C6) and (C7) provide polynomial transformations between the Tycho (BT-VT) and Cousins-Johnson (B-V) colors. The correct equation (C6), applicable over the color range (BT-VT)∈[0.40, 2.00], isB-V=(BT-VT)-7.813×10-3(BT-VT)-1.489×10-1(BT-VT)2+3.384×10-2(BT-VT)3. (C6)The correct formula (C6) was applied in the paper but transcribed incorrectly to the manuscript. Equation (C7) was published correctly, but the published color range is incorrect. For the color range (BT-VT)∈[-0.25, 0.40], equation (C7) should be used (same as published):B-V=(BT-VT)-0.006-1.069×10-1(BT-VT)+1.459×10-1(BT-VT)2. (C7)We thank John Carpenter for bringing the error in equation (C6) to our attention and François Bonnarel and Christian Nitschelm for pointing out the stellar misidentifications.

  2. NEAR-INFRARED VARIABILITY AMONG YOUNG STELLAR OBJECTS IN THE STAR FORMATION REGION CYGNUS OB7

    SciTech Connect

    Wolk, Scott J.; Rice, Thomas S.; Aspin, Colin

    2013-08-20

    We present an analysis of near-infrared time-series photometry in J, H, and K bands for about 100 epochs of a 1 Degree-Sign Multiplication-Sign 1 Degree-Sign region of the Lynds 1003/1004 dark cloud in the Cygnus OB7 region. Augmented by data from the Wide-field Infrared Survey Explorer, we identify 96 candidate disk bearing young stellar objects (YSOs) in the region. Of these, 30 are clearly Class I or earlier. Using the Wide-Field Imaging Camera on the United Kingdom Infrared Telescope, we were able to obtain photometry over three observing seasons, with photometric uncertainty better than 0.05 mag down to J Almost-Equal-To 17. We study detailed light curves and color trajectories of {approx}50 of the YSOs in the monitored field. We investigate the variability and periodicity of the YSOs and find the data are consistent with all YSOs being variable in these wavelengths on timescales of a few years. We divide the variability into four observational classes: (1) stars with periodic variability stable over long timescales, (2) variables which exhibit short-lived cyclic behavior, (3) long-duration variables, and (4) stochastic variables. Some YSO variability defies simple classification. We can explain much of the observed variability as being due to dynamic and rotational changes in the disk, including an asymmetric or changing blocking fraction, changes to the inner disk hole size, as well as changes to the accretion rate. Overall, we find that the Class I:Class II ratio of the cluster is consistent with an age of <1 Myr, with at least one individual, wildly varying source {approx}100, 000 yr old. We have also discovered a Class II eclipsing binary system with a period of 17.87 days.

  3. A possible OB protostar associated with the molecular outflow in G34.4

    NASA Astrophysics Data System (ADS)

    Bronfman, L.; May, J.; Nuernberger, D.; Shepherd, D.

    1999-10-01

    The most conspicuous massive molecular outflow candidate identified in our CS(2-1) survey of UC HII regions (Bronfman et al 1996) is G34.4 (IRAS 18507+0121) in the I Galactic quadrant. At a distance of 3.8 kpc, it is near (about 11') the very bright HII region G34.3 (Carral & Welch 1992), embedded in the same GMC with a VLSR of 57 km/s. The CS velocity profile obtained with SEST shows very broad wings, about 25 km/s wide at the 0.1 K level, indicating strong outflow activity. Near infrared images of the field, 90'' in size (0.35'' per pixel), obtained with the du Pont 100'' Telescope at Las Campanas, show a remarkably reddenned source visible only in the K' filter, elongated in shape, about 15'' in extent. We have recently observed the G34.4 region, using the OVRO array, in the 3 mm continuum band and in the H13CO+ line, at a resolution of 5''. Most of the H13CO+ flux (33.64 Jy) comes from two strong cores; while one of these cores is closely associated with the ! NIR source, the other one is associated with a single, unresolved continuum source that has a total flux of 56.8 mJy. The mass of gas and dust in this second, possibly "star-less" core is estimated from the millimeter continuum to be approximately 355 MSun, consistent with the presence of a massive, embedded OB protostar. Bronfman, L., May, J., & Nyman, L. 1996, A&AS 115, 81 Carral & Welch 1992, ApJ 385, 244

  4. Formate dehydrogenase from the methane oxidizer Methylosinus trichosporium OB3b.

    PubMed Central

    Yoch, D C; Chen, Y P; Hardin, M G

    1990-01-01

    Formate dehydrogenase (NAD+ dependent) was isolated from the obligate methanotroph Methylosinus trichosporium OB3b. When the enzyme was isolated anaerobically, two forms of the enzyme were seen on native polyacrylamide gels, DE-52 cellulose and Sephacryl S-300 columns; they were approximately 315,000 and 155,000 daltons. The enzyme showed two subunits on sodium dodecyl sulfate-polyacrylamide gels. The Mr of the alpha-subunit was 53,800 +/- 2,800, and that of the beta-subunit was 102,600 +/- 3,900. The enzyme (Mr 315,000) was composed of these subunits in an apparent alpha 2 beta 2 arrangement. Nonheme iron was present at a concentration ranging from 11 to 18 g-atoms per mol of enzyme (Mr 315,000). Similar levels of acid-labile sulfide were detected. No other metals were found in stoichiometric amounts. When the enzyme was isolated aerobically, there was no cofactor requirement for NAD reduction; however, when isolated anaerobically, activity was 80 to 90% dependent on the addition of flavin mononucleotide (FMN) to the reaction mixture. Furthermore, the addition of formate to an active, anoxic solution of formate dehydrogenase rapidly inactivated it in the absence of an electron acceptor; this activity could be reconstituted approximately 85% by 50 nM FMN. Flavin adenine dinucleotide could not replace FMN in reconstituting enzyme activity. The Kms of formate dehydrogenase for formate, NAD, and FMN were 146, 200, and 0.02 microM, respectively. "Pseudomonas oxalaticus" formate dehydrogenase, which has physical characteristics nearly identical to those of the M. trichosporium enzyme, was also shown to be inactivated under anoxic conditions by formate and reactivated by FMN. The evolutionary significance of this similarity is discussed. Images PMID:2376564

  5. Seismotectonics of the Gulf o Cadiz based on the NEAREST OBS experiment: mechanisms and faults

    NASA Astrophysics Data System (ADS)

    Silva, S.; Terrinha, P.; Matias, L.; Geissler, W.; Zitellini, N.; Romsdorf, M.; Nearest Working Group

    2012-04-01

    One of the main purposes of the NEAREST project (Integrated observation from NEAR shore sourcES of Tsunamis: towards an early warning system GOCE, contract n. 037110) was the identification and characterization of seismogenic and tsunamigenic structures in Gulf of Cadiz area, source region of the Lisbon 1755 earthquake and tsunami. To address this problem 24 broadband Ocean Bottom Seismometers (OBS) and a seafloor multiparametric station GEOSTAR (Geophysical and Oceanographic Station for Abyssal Research) acquired between September 2007 and July 2008 passive seismic data in this region. The results delivered a detailed investigation of the local seismicity, revealing 3 main clusters of earthquakes, two of them coinciding with the location of the 3 larger instrumental earthquakes in the area: i) the 28th February 1969 (Mw~8.0); ii) the 12th February 2007 (Mw=6.0) and iii) the 17th December 2009 (Mw=5.5). Most of the recorded events are located in the mantle (at depths between 30 and 60 km). However, focal mechanisms show a mixed pattern, mostly strike-slip and reverse dip-slip with a very few normal mechanisms. This implies the existence of tectonically active structures located much deeper than the ones mapped by Multichannel seismic reflection. A thorough analysis shows that the seismicity clusters are offset with respect to the upper crustal active thrusts. The wide-range solutions of focal mechanisms also imply that the related source processes are complex. It can reflect the interaction of different active geological structures and local rheological contrasts. To understand these new results in the context of the seismotectonics of the Gulf of Cadiz a review of some available geophysical data (reflection and refraction seismic profiles interpretation) in this area is presented. The results obtained in this work improves our knowledge about the local seismicity and related actives faults in the Gulf of Cadiz area, giving a new contribution to access to the

  6. ALMA Observations of Circumstellar Disks in the Upper Scorpius OB Association

    NASA Astrophysics Data System (ADS)

    Barenfeld, Scott A.; Carpenter, John M.; Ricci, Luca; Isella, Andrea

    2016-08-01

    We present ALMA observations of 106 G-, K-, and M-type stars in the Upper Scorpius OB Association hosting circumstellar disks. With these data, we measure the 0.88 mm continuum and 12CO J = 3–2 line fluxes of disks around low-mass (0.14–1.66 M ⊙) stars at an age of 5–11 Myr. Of the 75 primordial disks in the sample, 53 are detected in the dust continuum and 26 in CO. Of the 31 disks classified as debris/evolved transitional disks, five are detected in the continuum and none in CO. The lack of CO emission in approximately half of the disks with detected continuum emission can be explained if CO is optically thick but has a compact emitting area (≲40 au), or if the CO is heavily depleted by a factor of at least ˜1000 relative to interstellar medium abundances and is optically thin. The continuum measurements are used to estimate the dust mass of the disks. We find a correlation between disk dust mass and stellar host mass consistent with a power-law relation of {M}{dust}\\propto {M}* 1.67+/- 0.37. Disk dust masses in Upper Sco are compared to those measured in the younger Taurus star-forming region to constrain the evolution of disk dust mass. We find that the difference in the mean of {log}({M}{dust}/{M}* ) between Taurus and Upper Sco is 0.64 ± 0.09, such that M dust/M * is lower in Upper Sco by a factor of ˜4.5.

  7. Preparedness of Ob/Gyn residents for fellowship training in gynecologic oncology.

    PubMed

    Doo, David W; Powell, Matthew; Novetsky, Akiva; Sheeder, Jeanelle; Guntupalli, Saketh R

    2015-04-01

    Residency training in obstetrics and gynecology is being challenged by increasingly stringent regulations and decreased operative experience. We sought to determine the perception of preparedness of incoming gynecologic oncology fellows for advanced surgical training in gynecologic oncology. An online survey was sent to gynecologic oncologists involved in fellowship training in the United States. They were asked to evaluate their most recent incoming clinical fellows in the domains of professionalism, level of independence/graduated responsibility, psychomotor ability, clinical evaluation and management, and academia and scholarship using a standard Likert-style scale. The response rate among attending physicians was 40% (n = 105/260) and 61% (n = 28/46) for program directors. Of those who participated, 49% reported that their incoming fellows could not independently perform a hysterectomy, 59% reported that they could not independently perform 30 min of a major procedure, 40% reported that they could not control bleeding, 40% reported that they could not recognize anatomy and tissue planes, and 58% reported that they could not dissect tissue planes. Fellows lacked an understanding of pathophysiology, treatment recommendations, and the ability to identify and treat critically ill patients. In the academic domain, respondents agreed that fellows were deficient in the areas of protocol design (54%), statistical analysis (54%), and manuscript writing (65%). These results suggest that general Ob/Gyn residency is ineffective in preparing fellows for advanced training in gynecologic oncology and should prompt a revision of the goals and objectives of resident education to correct these deficiencies. PMID:26076160

  8. The Stellar Population of the M31 Spiral Arm around OB Association A24

    NASA Astrophysics Data System (ADS)

    Kodaira, Keiichi; Vansevičius, Vladas; Tamura, Motohide; Miyazaki, Satoshi

    1999-07-01

    A study of the stellar population of the M31 spiral arm around OB association A24 was carried out based on the photometric data obtained from deep V and JHK imaging. The luminosity function was obtained for -7<~Mbol<~-3.5 by applying the extinction correction corresponding to AV=1 and the bolometric correction BCK as an empirical function of (J-K)0. In comparing the observed color-luminosity diagrams with semitheoretical isochrones modified for the dust-shell effects, we found the young population of t<~30 Myr with supergiants of Mbol<~-5, the bulk of the intermediate-age population of t~0.2-2.5 Gyr with bright asymptotic giant branch (AGB) stars of -5<~Mbol<~-4, and old populations of t>~3 Gyr with AGB and red giant branch (RGB) stars of Mbol>~-4. The average star formation rate was estimated to be ~1.8×104 msolar Myr-1 and ~0.7×104 msolar Myr-1 per deprojected disk area of 1 kpc2 from the number density of B0 V stars around MV=-4.0 (age ~10 Myr) and the number density of bright AGB stars around Mbol=-4.3 (age ~1 Gyr), respectively. A study of the local variation in the V and the J and H luminosity functions revealed a kind of anticorrelation between the population of the young component and that of the intermediate-age component when subdomains of ~100 pc scales were concerned. This finding suggests that the disk domain around the A24 area experienced a series of star formation episodes alternatively among different subdomains with a timescale of several spiral passage periods. Brief discussions are given about the interstellar extinction and about the lifetimes of bright AGB stars and the highly red objects in the same area.

  9. EVIDENCE FOR EVOLUTION AMONG PRIMORDIAL DISKS IN THE 5 Myr OLD UPPER SCORPIUS OB ASSOCIATION

    SciTech Connect

    Dahm, S. E.

    2010-11-15

    Moderate-resolution, near-infrared spectra between 0.8 and 5.2 {mu}m were obtained for 12 late-type (K0-M3) disk-bearing members of the {approx}5 Myr old Upper Scorpius OB association using SpeX on the NASA Infrared Telescope Facility. For most sources, continuum excess emission first becomes apparent between {approx}2.2 and 4.5 {mu}m and is consistent with that produced by single-temperature blackbodies having characteristic temperatures ranging from {approx}500 to 1300 K. The near-infrared spectra for 5 of 12 Upper Scorpius sources exhibit Pa{gamma}, Pa{beta}, and Br{gamma} emission, indicators of disk accretion. Using a correlation between Pa{beta} and Br{gamma} emission line luminosity and accretion luminosity, mass accretion rates ( M-dot ) are derived for these sources that range from M-dot = 3.5x10{sup -10} to 1.5 x 10{sup -8} M{sub sun} yr{sup -1}. Merging the SpeX observations with Spitzer Space Telescope mid-infrared (5.4-37.0 {mu}m) spectroscopy and 24 and 70 {mu}m broadband photometry, the observed spectral energy distributions (SEDs) are compared with those predicted by two-dimensional, radiative transfer accretion disk models. Of the nine Upper Scorpius sources examined in this analysis, three exhibit SEDs that are most consistent with models having inner disk radii that substantially exceed their respective dust sublimation radii. The remaining Upper Scorpius members possess SEDs that either show significant dispersion among predicted inner disk radii or are best described by models having inner disk rims coincident with the dust sublimation radius.

  10. Seismic velocity variation along the Izu-Bonin arc estaimated from traveltime tomography using OBS data

    NASA Astrophysics Data System (ADS)

    Obana, K.; Tamura, Y.; Takahashi, T.; Kodaira, S.

    2014-12-01

    The Izu-Bonin (Ogasawara) arc is an intra-oceanic island arc along the convergent plate boundary between the subducting Pacific and overriding Philippine Sea plates. Recent active seismic studies in the Izu-Bonin arc reveal significant along-arc variations in crustal structure [Kodaira et al., 2007]. The thickness of the arc crust shows a remarkable change between thicker Izu (~30 km) and thinner Bonin (~10 km) arcs. In addition to this, several geological and geophysical contrasts, such as seafloor topography and chemical composition of volcanic rocks, between Izu and Bonin arc have been reported [e.g., Yuasa 1992]. We have conducted earthquake observations using ocean bottom seismographs (OBSs) to reveal seismic velocity structure of the crust and mantle wedge in the Izu-Bonin arc and to investigate origin of the along-arc structure variations. We deployed 40 short-period OBSs in Izu and Bonin area in 2006 and 2009, respectively. The OBS data were processed with seismic data recorded at routine seismic stations on Hachijo-jima, Aoga-shima, and Chichi-jima operated by National Research Institute for Earth Science and Disaster Prevention (NIED). More than 5000 earthquakes were observed during about three-months observation period in each experiment. We conducted three-dimensional seismic tomography using manually picked P- and S-wave arrival time data. The obtained image shows a different seismic velocity structures in the mantle beneath the volcanic front between Izu and Bonin arcs. Low P-wave velocity anomalies in the mantle beneath the volcanic front in the Izu arc are limited at depths deeper than those in the Bonin arc. On the other hand, P-wave velocity in the low velocity anomalies beneath volcanic front in the Bonin arc is slower than that in the Izu arc. These large-scale along-arc structure variations in the mantle could relate to the geological and geophysical contrasts between Izu and Bonin arcs.

  11. Hot stars observed by XMM-Newton. I. The catalog and the properties of OB stars

    NASA Astrophysics Data System (ADS)

    Nazé, Y.

    2009-11-01

    Aims: Following the advent of increasingly sensitive X-ray observatories, deep observations of early-type stars became possible. However, the results for only a few objects or clusters have until now been reported and there has been no large survey comparable to that based upon the ROSAT All-Sky Survey (RASS). Methods: A limited survey of X-ray sources, consisting of all public XMM-Newton observations (2XMMi) and slew survey data (XMMSL1), is now available. The X-ray counterparts to hot, massive stars have been searched for in these catalogs. Results: About 300 OB stars were detected with XMM-Newton. Half of them were bright enough for a spectral analysis to be possible, and we make available the detailed spectral properties that were derived. The X-ray spectra of O stars are represented well by low (<1 keV) temperature components and seem to indicate that an absorption column is present in addition to the interstellar contribution. The X-ray fluxes are well correlated with the bolometric fluxes, with a scatter comparable to that of the RASS studies and thus larger than found previously with XMM-Newton for some individual clusters. These results contrast with those of B stars that exhibit a large scatter in the LX - LBOL relation, no additional absorption being found, and the fits indicate a plasma at higher temperatures. Variability (either within one exposure or between multiple exposures) was also investigated whenever possible: short-term variations are far more rare than long-term ones (the former affects a few percent of the sample, while the latter concerns between one third and two thirds of the sources). Conclusions: This paper presents the results of the first high-sensitivity investigation of the overall high-energy properties of a sizable sample of hot stars. Based on observations collected with XMM-Newton, an ESA Science Mission with instruments and contributions directly funded by ESA Member States and the USA (NASA), and accessed via the 2XMMi and

  12. Breakup Style and Magmatic Underplating West of the Lofoten Islands, Norway, Based on OBS Data.

    NASA Astrophysics Data System (ADS)

    Breivik, A. J.; Faleide, J. I.; Mjelde, R.; Murai, Y.; Flueh, E. R.

    2014-12-01

    The breakup of the Northeast Atlantic in the Early Eocene was magma-rich, forming the major part of the North Atlantic Igneous Province (NAIP). This is seen as extrusive and intrusive magmatism in the continental domain, and as a thicker than normal oceanic crust produced the first few million years after continental breakup. The maximum magma productivity and the duration of excess magmatism varies along the margins of Northwest Europe and East Greenland, to some extent as a function of the distance from the Iceland hotspot. The Vøring Plateau off mid-Norway is the northernmost of the margin segments in northwestern Europe with extensive magmatism. North of the plateau, magmatism dies off towards the Lofoten Margin, marking the northern boundary of the NAIP here. In 2003, as part of the Euromargins Program we collected an Ocean Bottom Seismometer (OBS) profile from mainland Norway, across the Lofoten Islands, and out into the deep ocean. Forward velocity modeling using raytracing reveals a continental margin that shows transitional features between magma-rich and magma-poor rifting. On one hand, we detect an up to 2 km thick and 40-50 km wide magmatic underplate of the outer continent, on the other hand, continental thinning is greater and intrusive magmatism less than farther south. Continental breakup also appears to be somewhat delayed compared to breakup on the Vøring Plateau, consistent with increased extension. This indicates that magmatic diking, believed to quickly lead to continental breakup of volcanic margins and thus to reduce continental thinning, played a much lesser role here than at the plateau. Early post-breakup oceanic crust is up to 8 km thick, less than half of that observed farther south. The most likely interpretation of these observations, is that the source for the excess magmatism of the NAIP was not present at the Lofoten Margin during rifting, and that the excess magmatism actually observed was the result of lateral transport from the

  13. The Anisotropic Structure of South China Sea: Using OBS Data to Constrain Mantle Flow

    NASA Astrophysics Data System (ADS)

    Li, L.; Xue, M.; Yang, T.; Liu, C.; Hua, Q.; Xia, S.; Huang, H.; Le, B. M.; Huo, D.; Pan, M.

    2015-12-01

    The dynamic mechanism of the formation of South China Sea (SCS) has been debated for decades. The anisotropic structure can provide useful insight into the complex evolution of SCS by indicating its mantle flow direction and strength. In this study, we employ shear wave splitting methods on two half-year seismic data collected from 10 and 6 passive source Ocean Bottom Seismometers (OBS) respectively. These OBSs were deployed along both sides of the extinct ridge in the central basin of SCS by Tongji University in 2012 and 2013 respectively, which were then successfully recovered in 2013 and 2015 respectively. Through processing and inspecting the global and regional earthquakes (with local events being processing) of the 2012 dataset, measurements are made for 2 global events and 24 regional events at 5 OBSs using the tangential energy minimization, the smallest eigenvalue minimization, as well as the correlation methods. We also implement cluster analysis on the splitting results obtained for different time windows as well as filtered at different frequency bands. For teleseismic core phases like SKS and PKS, we find the fast polarization direction beneath the central basin is approximately NE-SW, nearly parallel to the extinct ridge in the central basin of SCS. Whereas for regional events, the splitting analysis on S, PS and ScS phases shows much more complicated fast directions as the ray path varies for different phases. The fast directions observed can be divided into three groups: (1) for the events from the Eurasia plate, a gradual rotation of the fast polarization direction from NNE-SSW to NEE-SWW along the path from the inner Eurasia plate to the central SCS is observed, implying the mantle flow is controlled by the India-Eurasia collision; (2) for the events located at the junction of Pacific plate and Philippine plate, the dominant fast direction is NW-SE, almost perpendicular to Ryukyu Trench as well as sub-parallel to the absolute direction of

  14. HD 144548: A young triply eclipsing system in the Upper Scorpius OB association

    NASA Astrophysics Data System (ADS)

    Alonso, R.; Deeg, H. J.; Hoyer, S.; Lodieu, N.; Palle, E.; Sanchis-Ojeda, R.

    2015-12-01

    The star HD 144548 (=HIP 78977; TYP 6212-1273-1) has been known as a detached eclipsing binary and a bona-fide member of the Upper Scorpius OB association. Continuous photometry from the K2 mission on Campaign Two has revealed the presence of additional eclipses due to the presence of a third star in the system. These are explained by a system composed of the two previously known members of the eclipsing system (Ba and Bb) with a period of 1.63 d, orbiting around an F7-F8V star with a period of 33.945 ± 0.002 d in an eccentric orbit (eA = 0.2652 ± 0.0003). The timing of the eclipses of Ba and Bb reveals the same 33.9 d periodicity, which we interpret as the combination of a light time effect combined with dynamical perturbations on the close system. Here we combine radial velocities and analytical approximations for the timing of the eclipses to derive masses and radii for the three components of the system. We obtain a mass of 1.44 ± 0.04 M⊙ and radius of 2.41 ± 0.03 R⊙ for the A component, and almost identical masses and radii of about 0.96 M⊙ and 1.33 R⊙ for each of the two components of the close binary. HD 144548 is the first triply eclipsing system for which radial velocities of all components could be measured. Partially based on observations made with the Italian Telescopio Nazionale Galileo (TNG) operated by the Fundación Galileo Galilei of the INAF, the Nordic Optical Telescope, operated by the Nordic Optical Telescope Scientific Association, and the William Herschel Telescope (programme DDT58 - PI Lodieu) operated by the Isaac Newton Group on the island of La Palma at the Spanish Observatorio Roque de los Muchachos of the IAC. This paper includes data collected by the Kepler mission. Funding for the Kepler mission is provided by the NASA Science Mission directorate.Appendices are available in electronic form at http://www.aanda.org

  15. Deep Stucture of the Northwestern Atlantic Moroccan Margin Studied by OBS and Deep Multichannel Seismic Reflection.

    NASA Astrophysics Data System (ADS)

    MALOD, J. A.; Réhault, J.; Sahabi, M.; Géli, L.; Matias, L.; Diaz, J.; Zitellini, N.

    2001-12-01

    The Northwestern Atlantic Moroccan margin, a conjugate of the New Scotland margin, is one of the oldest passive margin of the world. Continental break up occurred at early Liassic time and the deep margin is characterized by a large salt basin. A good knowledge of this basin is of major interest to improve the initial reconstruction between Africa, North America and Iberia (Eurasia). It is also a good opportunity to study a mature passive margin and model its structure and evolution.Moreover, there is a need to assess the geological hazards linked to the neotectonic activity within the Africa-Eurasia plate boundary. These topics have been adressed during the SISMAR cruise carried out from April 9th to May 4th 2001.During this cruise, 3667 km of multichannel seismic reflection (360 channels, 4500 m long streamer, 4800 ci array of air guns) were recorded together with refraction records by means of 48 OBH/OBS drops. Simultaneously, some of the marine profiles have been extended onshore with 16 portable seismic land stations. We present the initial results of this study. Off El Jadida, the Moho and structures within the thinned continental crust are well imaged on both the reflection and refraction records. In the northern area, off Casablanca, we follow the deepening of the moroccan margin beneath the up to 9 sec (twtt) allochtonous series forming a prism at the front the Rif-Betic chain. Sismar cruise has been also the opportunity to record long seismic profiles making the junction between the Portuguese margin and the Moroccan one, and crossing the Iberian-African plate boundary. This allows to observe the continuity of the sedimentary sequence after the end of the large inter-plate motion in Early Cretaceous. In addition to the authors, SISMAR Group includes: AMRHAR Mostafa, BERMUDEZ VASQUEZ Antoni, CAMURRI Francesca, CONTRUCCI Isabelle, CORELA Carlos, DIAZ Jordi, DORVAL Philippe, EL ARCHI Abdelkrim, EL ATTARI Ahmed, GONZALEZ Raquel, HARMEGNIES Francois, JAFFAL

  16. AN UPDATED LOOK AT BINARY CHARACTERISTICS OF MASSIVE STARS IN THE CYGNUS OB2 ASSOCIATION

    SciTech Connect

    Kiminki, Daniel C.; Kobulnicky, Henry A.

    2012-05-20

    This work provides a statistical analysis of the massive star binary characteristics in the Cygnus OB2 association using radial velocity information of 114 B3-O5 primary stars and orbital properties for the 24 known binaries. We compare these data to a series of Monte Carlo simulations to infer the intrinsic binary fraction and distributions of mass ratios, periods, and eccentricities. We model the distribution of mass ratio, log-period, and eccentricity as power laws and find best-fitting indices of {alpha} = 0.1 {+-} 0.5, {beta} = 0.2 {+-} 0.4, and {gamma} = -0.6 {+-} 0.3, respectively. These distributions indicate a preference for massive companions, short periods, and low eccentricities. Our analysis indicates that the binary fraction of the cluster is 44% {+-} 8% if all binary systems are (artificially) assumed to have P < 1000 days; if the power-law period distribution is extrapolated to 10{sup 4} years, then a plausible upper limit for bound systems, the binary fraction is {approx}90% {+-} 10%. Of these binary (or higher order) systems, {approx}45% will have companions close enough to interact during pre- or post-main-sequence evolution (semi-major axis {approx}<4.7 AU). The period distribution for P < 26 days is not well reproduced by any single power law owing to an excess of systems with periods around 3-5 days (0.08-0.31 AU) and a relative shortage of systems with periods around 7-14 days (0.14-0.62 AU). We explore the idea that these longer-period systems evolved to produce the observed excess of short-period systems. The best-fitting binary parameters imply that secondaries generate, on average, {approx}16% of the V-band light in young massive populations. This means that photometrically based distance measurements for young massive clusters and associations will be systematically low by {approx}8% (0.16 mag in the distance modulus) if the luminous contributions of unresolved secondaries are not taken into account.

  17. MY Serpentis: a high-mass triple system in the Ser OB2 association

    NASA Astrophysics Data System (ADS)

    Ibanoglu, C.; Çakırlı, Ö.; Sipahi, E.

    2013-11-01

    We present spectroscopic observations of the massive multiple system HD 167971, located in the open cluster NGC 6604. The brighter component of the triple system is the overcontact eclipsing binary MY Ser with an orbital period of 3.32 d. The radial velocities and the previously published UBV data obtained by Mayer et al. and the UBVRI light curves by Davidge & Forbes are analysed for the physical properties of the components. We determine the following absolute parameters: for the primary star Mp = 32.23 ± 0.54 M⊙, Rp = 14.23 ± 0.75 R⊙; and for the secondary star Ms = 30.59 ± 0.53 M⊙, Rs = 13.89 ± 0.75 R⊙. Photoelectric times of minimum light are analysed under the consideration of the light-time orbit. The centre-of-mass of the eclipsing binary is orbiting around the common centre-of-gravity of the triple system with a period of 21.2 ± 0.7 yr and with a projected semi-major axis of 5.5 ± 0.7 au. The mass function for the third star was calculated as 0.370 ± 0.036 M⊙. The light contributions of the third star to the triple system in the UBV passbands were derived and the intrinsic magnitudes and colours were calculated individually for the three stars. The components of the eclipsing pair were classified as O7.5III and O9.5III. The intrinsic colour indices for the third star yield a spectral type of (O9.5-B0)III-I. This classification leads to constrain the inclination of the third-body orbit, which should be about 30°, and therefore its mass should be about 29 M⊙. MY Ser is one of the rare massive O-type triple system at a distance of 1.65 ± 0.13 kpc, the same as for the NGC 6604 embedded in the Ser OB2 association.

  18. Investigating the potential magnetic origin of wind variability in OB stars

    NASA Astrophysics Data System (ADS)

    David-Uraz, Alexandre

    2016-05-01

    In this thesis, the origin of large-scale structures in hot star winds, believed to be responsible for the presence of discrete absorption components (DACs) in the absorption troughs of ultraviolet resonance lines, is constrained using both observations and numerical simulations. These structures are understood as arising from bright regions on the stellar surface, although their physical cause remains unknown. First, we use high quality circular spectropolarimetric observations of 13 well-studied OB stars to evaluate the potential role of dipolar magnetic fields in producing DACs. We perform longitudinal field measurements and place limits on the field strength using Bayesian inference, assuming that it is dipolar. No magnetic field was detected within this sample. The derived constraints statistically refute any significant dynamical influence from a magnetic dipole on the wind for all of these stars, ruling out such fields as a cause for DACs. Second, we perform numerical simulations using bright spots constrained by broadband optical photometric observations. We calculate hydrodynamical wind models using three sets of spot sizes and strengths. Co-rotating interaction regions are yielded in each model, and radiative transfer shows that the properties of the variations in the UV resonance lines synthesized from these models are consistent with those found in observed UV spectra, establishing the first consistent link between UV spectroscopic line profile variability and photometric variations and thus supporting the bright spot paradigm (BSP). Finally, we develop and apply a phenomenological model to quantify the measurable effects co-rotating bright spots would have on broadband optical photometry and on the profiles of photospheric lines in optical spectra. This model can be used to evaluate the existence of these spots, and, in the event of their detection, characterize them. Furthermore, a tentative spot evolution model is presented. A preliminary analysis of

  19. NEAR-INFRARED VARIABILITY IN YOUNG STARS IN CYGNUS OB7

    SciTech Connect

    Rice, Thomas S.; Wolk, Scott J.; Aspin, Colin

    2012-08-10

    We present the first results from a 124 night J, H, K near-infrared monitoring campaign of the dark cloud L 1003 in Cygnus OB7, an active star-forming region. Using three seasons of UKIRT observations spanning 1.5 years, we obtained high-quality photometry on 9200 stars down to J = 17 mag, with photometric uncertainty better than 0.04 mag. On the basis of near-infrared excesses from disks, we identify 30 pre-main-sequence stars, including 24 which are newly discovered. We analyze those stars and find that the NIR excesses are significantly variable. All 9200 stars were monitored for photometric variability; among the field star population, {approx}160 exhibited near-infrared variability (1.7% of the sample). Of the 30 young stellar objects (YSOs), 28 of them (93%) are variable at a significant level. Of the 30 YSOs, twenty-five have near-infrared excess consistent with simple disk-plus-star classical T Tauri models. Nine of these (36%) drift in color space over the course of these observations and/or since Two Micron All Sky Survey observations such that they cross the boundary defining the NIR excess criteria; effectively, they have a transient near-infrared excess. Thus, time-series JHK observations can be used to obtain a more complete sample of disk-bearing stars than single-epoch JHK observations. About half of the YSOs have color-space variations parallel to either the classical T Tauri star locus or a hybrid track which includes the dust reddening trajectory. This indicates that the NIR variability in YSOs that possess accretion disks arises from a combination of variable extinction and changes in the inner accretion disk: either in accretion rate, central hole size, and/or the inclination of the inner disk. While some variability may be due to stellar rotation, the level of variability on the individual stars can exceed a magnitude. This is a strong empirical suggestion that protoplanetary disks are quite dynamic and exhibit more complex activity on short

  20. A TonB-Dependent Transporter Is Responsible for Methanobactin Uptake by Methylosinus trichosporium OB3b.

    PubMed

    Gu, Wenyu; Farhan Ul Haque, Muhammad; Baral, Bipin S; Turpin, Erick A; Bandow, Nathan L; Kremmer, Elisabeth; Flatley, Andrew; Zischka, Hans; DiSpirito, Alan A; Semrau, Jeremy D

    2016-01-01

    Methanobactin, a small modified polypeptide synthesized by methanotrophs for copper uptake, has been found to be chromosomally encoded. The gene encoding the polypeptide precursor of methanobactin, mbnA, is part of a gene cluster that also includes several genes encoding proteins of unknown function (but speculated to be involved in methanobactin formation) as well as mbnT, which encodes a TonB-dependent transporter hypothesized to be responsible for methanobactin uptake. To determine if mbnT is truly responsible for methanobactin uptake, a knockout was constructed in Methylosinus trichosporium OB3b using marker exchange mutagenesis. The resulting M. trichosporium mbnT::Gm(r) mutant was found to be able to produce methanobactin but was unable to internalize it. Further, if this mutant was grown in the presence of copper and exogenous methanobactin, copper uptake was significantly reduced. Expression of mmoX and pmoA, encoding polypeptides of the soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO), respectively, also changed significantly when methanobactin was added, which indicates that the mutant was unable to collect copper under these conditions. Copper uptake and gene expression, however, were not affected in wild-type M. trichosporium OB3b, indicating that the TonB-dependent transporter encoded by mbnT is responsible for methanobactin uptake and that methanobactin is a key mechanism used by methanotrophs for copper uptake. When the mbnT::Gm(r) mutant was grown under a range of copper concentrations in the absence of methanobactin, however, the phenotype of the mutant was indistinguishable from that of wild-type M. trichosporium OB3b, indicating that this methanotroph has multiple mechanisms for copper uptake. PMID:26773085

  1. Glacier Area and River Runoff Changes in the Head of Ob River Basins During the Last 50 Years

    NASA Astrophysics Data System (ADS)

    Surazakov, A. B.; Aizen, V. B.; Aizen, E. M.; Nikitin, S. A.; Narojniy, J. K.

    2006-12-01

    The Altai mountains in Siberia define southern periphery of the Asian Arctic Basin, and the Ob River is a major Siberian river fed by fresh water from Altai glaciers. Intensification of glacier melt in the head of Ob River since the middle of 20th century may have a considerable influence on the water resources and hydrological regime of Siberian rivers, and freshwater budget of the Arctic Ocean. In our research we estimated glacier area and runoff changes in the Aktru River basin (34.9 km2, 45% covered by glaciers) in the Central Altai using remote sensing data and in situ glaciological and hydrological observations. The measurements of the glacier mass-balance started in this basin in 1952 as a part of the World Glacier Monitoring Service, however an accurate estimation of the glacier area change in the last two decades have not been accomplished. In our research we used aerial photographs (1952, 1975), Corona (1968) and ASTER (2004) images, and Ground Control Points collected with DGPS in 2005 and 2006 field surveys. Preliminary analysis shows that area of the studied glaciers reduced up to 7% and glacier tongues retreated up to 600 m from 1952 to 2004. The rate of the glacier recession doubled between 1975 and 2004 and the river runoff increased by 30 mm/year at the head of Ob river tributaries fed by snow and glacier melt water. During the period from 1954 to 2004 annual (mainly summer) air temperature increased by 0.1 C° a decade and precipitation (mainly spring and summer) increased by 50 mm at an elevation of 2000 m.

  2. Sub-crustal earthquakes beneath the Gulf of Cadiz - First results from seismological observations with the NEAREST OBS network

    NASA Astrophysics Data System (ADS)

    Geissler, W. H.; Matias, L. M.; Monna, S.; Stich, D.; Iben Brahim, A.; Mancilla, F.; Zitellini, N.; Nearest Working Group

    2009-04-01

    The geophysical and geological investigations conducted so far in the Gulf of Cadiz allow us today to have an idea of the largest active faults that can generate destructive earthquakes and tsunamis comparable to the Nov 1st, 1755 Lisbon event. However, their kinematics and seismic activity are poorly known because the seismic networks based on land do not allow a precise hypocenter location and estimation of focal mechanisms for the smaller events. Therefore the EC project NEAREST (Integrated observation from NEAR shore sourcES of Tsunamis: towards an early warning system) was initiated (GOCE, contract n. 037110). One of the main objectives of the project is the characterisation of the tsunamigenic sources in the Gulf of Cadiz through seismological monitoring of natural seismicity by means of 24 BB seismometers deployed for 11 months in addition to the GEOSTAR multi-parameter deep-sea observatory. Together with the dense onshore seismic networks the temporary OBS network will allow the location and characterization of small seismic events more precisely than it can be done with onshore stations only. Spectrograms are used to identify previously unknown earthquakes. One of the major questions is the maximum depth of seismic activity beneath the Gulf of Cadiz. In the recording period from September 2007 to August 2008 about 300 events were located within the OBS network using the Portuguese onshore seismic stations. Magnitudes range from 1 to 4.7 (ML from Institute of Meteorology Lisbon, Portugal). Using the OBS network many events could be detected which are not located by the onshore stations. First results show that the events occur to approximately 50 km depth, often deeper than the locations by land stations, and confirming the results available from regional and teleseismic waveform modelling. Focal mechanisms show strike-slip and thrust-slip events.

  3. The Gaia-ESO survey: Discovery of a spatially extended low-mass population in the Vela OB2 association

    NASA Astrophysics Data System (ADS)

    Sacco, G. G.; Jeffries, R. D.; Randich, S.; Franciosini, E.; Jackson, R. J.; Cottaar, M.; Spina, L.; Palla, F.; Mapelli, M.; Alfaro, E. J.; Bonito, R.; Damiani, F.; Frasca, A.; Klutsch, A.; Lanzafame, A.; Bayo, A.; Barrado, D.; Jiménez-Esteban, F.; Gilmore, G.; Micela, G.; Vallenari, A.; Allende Prieto, C.; Flaccomio, E.; Carraro, G.; Costado, M. T.; Jofré, P.; Lardo, C.; Magrini, L.; Morbidelli, L.; Prisinzano, L.; Sbordone, L.

    2015-02-01

    The nearby (distance ~ 350-400 pc), rich Vela OB2 association, includes γ2 Velorum, one of the most massive binaries in the solar neighbourhood and an excellent laboratory for investigating the formation and early evolution of young clusters. Recent Gaia-ESO survey observations have led to the discovery of two kinematically distinct populations in the young (10-15 Myr) cluster immediately surrounding γ2 Velorum. Here we analyse the results of Gaia-ESO survey observations of NGC 2547, a 35 Myr cluster located two degrees south of γ2 Velorum. The radial velocity distribution of lithium-rich pre-main sequence stars shows a secondary population that is kinematically distinct from and younger than NGC 2547. The radial velocities, lithium absorption lines, and the positions in a colour-magnitude diagram of this secondary population are consistent with those of one of the components discovered around γ2 Velorum. This result shows that there is a young, low-mass stellar population spread over at least several square degrees in the Vela OB2 association. This population could have originally been part of a cluster around γ2 Velorum that expanded after gas expulsion or formed in a less dense environment that is spread over the whole Vela OB2 region. Based on observations made with the ESO/VLT, at Paranal Observatory, under program 188.B-3002 (The Gaia-ESO Public Spectroscopic Survey).Table 1 is only available at the CDS via anonymous ftp to http://cdsarc.u-strasbg.fr (ftp://130.79.128.5) or via http://cdsarc.u-strasbg.fr/viz-bin/qcat?J/A+A/574/L7

  4. Search for OB stars running away from young star clusters. II. The NGC 6357 star-forming region

    NASA Astrophysics Data System (ADS)

    Gvaramadze, V. V.; Kniazev, A. Y.; Kroupa, P.; Oh, S.

    2011-11-01

    Dynamical few-body encounters in the dense cores of young massive star clusters are responsible for the loss of a significant fraction of their massive stellar content. Some of the escaping (runaway) stars move through the ambient medium supersonically and can be revealed via detection of their bow shocks (visible in the infrared, optical or radio). In this paper, which is the second of a series of papers devoted to the search for OB stars running away from young ( ≲ several Myr) Galactic clusters and OB associations, we present the results of the search for bow shocks around the star-forming region NGC 6357. Using the archival data of the Midcourse Space Experiment (MSX) satellite and the Spitzer Space Telescope, and the preliminary data release of the Wide-Field Infrared Survey Explorer (WISE), we discovered seven bow shocks, whose geometry is consistent with the possibility that they are generated by stars expelled from the young (~1-2 Myr) star clusters, Pismis 24 and AH03 J1725-34.4, associated with NGC 6357. Two of the seven bow shocks are driven by the already known OB stars, HD 319881 and [N78] 34. Follow-up spectroscopy of three other bow-shock-producing stars showed that they are massive (O-type) stars as well, while the 2MASS photometry of the remaining two stars suggests that they could be B0 V stars, provided that both are located at the same distance as NGC 6357. Detection of numerous massive stars ejected from the very young clusters is consistent with the theoretical expectation that star clusters can effectively lose massive stars at the very beginning of their dynamical evolution (long before the second mechanism for production of runaway stars, based on a supernova explosion in a massive tight binary system, begins to operate) and lends strong support to the idea that probably all field OB stars have been dynamically ejected from their birth clusters. A by-product of our search for bow shocks around NGC 6357 is the detection of three circular

  5. Cloud Structure of Galactic OB Cluster-forming Regions from Combining Ground- and Space-based Bolometric Observations

    NASA Astrophysics Data System (ADS)

    Lin, Yuxin; Liu, Hauyu Baobab; Li, Di; Zhang, Zhi-Yu; Ginsburg, Adam; Pineda, Jaime E.; Qian, Lei; Galván-Madrid, Roberto; McLeod, Anna Faye; Rosolowsky, Erik; Dale, James E.; Immer, Katharina; Koch, Eric; Longmore, Steve; Walker, Daniel; Testi, Leonardo

    2016-09-01

    We have developed an iterative procedure to systematically combine the millimeter and submillimeter images of OB cluster-forming molecular clouds, which were taken by ground-based (CSO, JCMT, APEX, and IRAM-30 m) and space telescopes (Herschel and Planck). For the seven luminous (L\\gt {10}6 L ⊙) Galactic OB cluster-forming molecular clouds selected for our analyses, namely W49A, W43-Main, W43-South, W33, G10.6-0.4, G10.2-0.3, and G10.3-0.1, we have performed single-component, modified blackbody fits to each pixel of the combined (sub)millimeter images, and the Herschel PACS and SPIRE images at shorter wavelengths. The ∼10″ resolution dust column density and temperature maps of these sources revealed dramatically different morphologies, indicating very different modes of OB cluster-formation, or parent molecular cloud structures in different evolutionary stages. The molecular clouds W49A, W33, and G10.6-0.4 show centrally concentrated massive molecular clumps that are connected with approximately radially orientated molecular gas filaments. The W43-Main and W43-South molecular cloud complexes, which are located at the intersection of the Galactic near 3 kpc (or Scutum) arm and the Galactic bar, show a widely scattered distribution of dense molecular clumps/cores over the observed ∼10 pc spatial scale. The relatively evolved sources G10.2-0.3 and G10.3-0.1 appear to be affected by stellar feedback, and show a complicated cloud morphology embedded with abundant dense molecular clumps/cores. We find that with the high angular resolution we achieved, our visual classification of cloud morphology can be linked to the systematically derived statistical quantities (i.e., the enclosed mass profile, the column density probability distribution function (N-PDF), the two-point correlation function of column density, and the probability distribution function of clump/core separations). In particular, the massive molecular gas clumps located at the center of G10

  6. Globules and pillars in Cygnus X. I. Herschel far-infrared imaging of the Cygnus OB2 environment

    NASA Astrophysics Data System (ADS)

    Schneider, N.; Bontemps, S.; Motte, F.; Blazere, A.; André, Ph.; Anderson, L. D.; Arzoumanian, D.; Comerón, F.; Didelon, P.; Di Francesco, J.; Duarte-Cabral, A.; Guarcello, M. G.; Hennemann, M.; Hill, T.; Könyves, V.; Marston, A.; Minier, V.; Rygl, K. L. J.; Röllig, M.; Roy, A.; Spinoglio, L.; Tremblin, P.; White, G. J.; Wright, N. J.

    2016-06-01

    The radiative feedback of massive stars on molecular clouds creates pillars, globules and other features at the interface between the H II region and molecular cloud. Optical and near-infrared observations from the ground as well as with the Hubble or Spitzer satellites have revealed numerous examples of such cloud structures. We present here Herschel far-infrared observations between 70 μm and 500 μm of the immediate environment of the rich Cygnus OB2 association, performed within the Herschel imaging survey of OB Young Stellar objects (HOBYS) program. All of the observed irradiated structures were detected based on their appearance at 70 μm, and have been classified as pillars, globules, evaporating gasous globules (EGGs), proplyd-like objects, and condensations. From the 70 μm and 160 μm flux maps, we derive the local far-ultraviolet (FUV) field on the photon dominated surfaces. In parallel, we use a census of the O-stars to estimate the overall FUV-field, that is 103-104 G0 (Habing field) close to the central OB cluster (within 10 pc) and decreases down to a few tens G0, in a distance of 50 pc. From a spectral energy distribution (SED) fit to the four longest Herschel wavelengths, we determine column density and temperature maps and derive masses, volume densities and surface densities for these structures. We find that the morphological classification corresponds to distinct physical properties. Pillars and globules are massive (~500 M⊙) and large (equivalent radius r ~ 0.6 pc) structures, corresponding to what is defined as "clumps" for molecular clouds. EGGs and proplyd-likeobjects are smaller (r ~ 0.1 and 0.2 pc) and less massive (~10 and ~30 M⊙). Cloud condensations are small (~0.1 pc), have an average mass of 35 M⊙, are dense (~6 × 104 cm-3), and can thus be described as molecular cloud "cores". All pillars and globules are oriented toward the Cyg OB2 association center and have the longest estimated photoevaporation lifetimes, a few million

  7. The young open cluster NGC 3293 and its relation to Car OB1 and the Carina Nebula complex

    NASA Technical Reports Server (NTRS)

    Turner, D. G.; Grieve, G. R.; Herbst, W.; Harris, W. E.

    1980-01-01

    On the basis of visible evidence, NGC 3293 appears to be associated with a number of objects in the Carina Nebula complex. UBVRI photoelectric photometry and MK-spectral specifications are presented for a large number of stars in this cluster and its surroundings (Car OB1). Over 70 cluster members with spectral types earlier than B9 are identified. Star counts indicate that the expected number of members brighter than V = 18 is 297 plus or minus 9. The cluster appears to be situated just in front of a dense dust lane, which itself appears to be part of the dust complex emanating from the center of the Carina Nebula.

  8. [The Change Detection of High Spatial Resolution Remotely Sensed Imagery Based on OB-HMAD Algorithm and Spectral Features].

    PubMed

    Chen, Qiang; Chen, Yun-hao; Jiang, Wei-guo

    2015-06-01

    The high spatial resolution remotely sensed imagery has abundant detailed information of earth surface, and the multi-temporal change detection for the high resolution remotely sensed imagery can realize the variations of geographical unit. In terms of the high spatial resolution remotely sensed imagery, the traditional remote sensing change detection algorithms have obvious defects. In this paper, learning from the object-based image analysis idea, we proposed a semi-automatic threshold selection algorithm named OB-HMAD (object-based-hybrid-MAD), on the basis of object-based image analysis and multivariate alternative detection algorithm (MAD), which used the spectral features of remotely sensed imagery into the field of object-based change detection. Additionally, OB-HMAD algorithm has been compared with other the threshold segmentation algorithms by the change detection experiment. Firstly, we obtained the image object by the multi-solution segmentation algorithm. Secondly, we got the object-based difference image object using MAD and minimum noise fraction rotation (MNF) for improving the SNR of the image object. Then, the change objects or area are classified using histogram curvature analysis (HCA) method for the semi-automatic threshold selection, which determined the threshold by calculated the maximum value of curvature of the histogram, so the HCA algorithm has better automation than other threshold segmentation algorithms. Finally, the change detection results are validated using confusion matrix with the field sample data. Worldview-2 imagery of 2012 and 2013 in case study of Beijing were used to validate the proposed OB-HMAD algorithm. The experiment results indicated that OB-HMAD algorithm which integrated the multi-channel spectral information could be effectively used in multi-temporal high resolution remotely sensed imagery change detection, and it has basically solved the "salt and pepper" problem which always exists in the pixel-based change

  9. Evidence of the Galactic outer ring R1R2' from young open clusters and OB-associations

    NASA Astrophysics Data System (ADS)

    Mel'nik, A. M.; Rautiainen, P.; Glushkova, E. V.; Dambis, A. K.

    2016-02-01

    The distribution of young open clusters in the Galactic plane within 3 kpc from the Sun suggests the existence of the outer ring R1R2' in the Galaxy. The optimum value of the solar position angle with respect to the major axis of the bar, θb, providing the best agreement between the distribution of open clusters and model particles is θb=35±10°. The kinematical features obtained for young open clusters and OB-associations with negative Galactocentric radial velocity VR indicate the solar location near the descending segment of the outer ring R2.

  10. Cloud Structure of Galactic OB Cluster-forming Regions from Combining Ground- and Space-based Bolometric Observations

    NASA Astrophysics Data System (ADS)

    Lin, Yuxin; Liu, Hauyu Baobab; Li, Di; Zhang, Zhi-Yu; Ginsburg, Adam; Pineda, Jaime E.; Qian, Lei; Galván-Madrid, Roberto; McLeod, Anna Faye; Rosolowsky, Erik; Dale, James E.; Immer, Katharina; Koch, Eric; Longmore, Steve; Walker, Daniel; Testi, Leonardo

    2016-09-01

    We have developed an iterative procedure to systematically combine the millimeter and submillimeter images of OB cluster-forming molecular clouds, which were taken by ground-based (CSO, JCMT, APEX, and IRAM-30 m) and space telescopes (Herschel and Planck). For the seven luminous (L\\gt {10}6 L ⊙) Galactic OB cluster-forming molecular clouds selected for our analyses, namely W49A, W43-Main, W43-South, W33, G10.6-0.4, G10.2-0.3, and G10.3-0.1, we have performed single-component, modified blackbody fits to each pixel of the combined (sub)millimeter images, and the Herschel PACS and SPIRE images at shorter wavelengths. The ˜10″ resolution dust column density and temperature maps of these sources revealed dramatically different morphologies, indicating very different modes of OB cluster-formation, or parent molecular cloud structures in different evolutionary stages. The molecular clouds W49A, W33, and G10.6-0.4 show centrally concentrated massive molecular clumps that are connected with approximately radially orientated molecular gas filaments. The W43-Main and W43-South molecular cloud complexes, which are located at the intersection of the Galactic near 3 kpc (or Scutum) arm and the Galactic bar, show a widely scattered distribution of dense molecular clumps/cores over the observed ˜10 pc spatial scale. The relatively evolved sources G10.2-0.3 and G10.3-0.1 appear to be affected by stellar feedback, and show a complicated cloud morphology embedded with abundant dense molecular clumps/cores. We find that with the high angular resolution we achieved, our visual classification of cloud morphology can be linked to the systematically derived statistical quantities (i.e., the enclosed mass profile, the column density probability distribution function (N-PDF), the two-point correlation function of column density, and the probability distribution function of clump/core separations). In particular, the massive molecular gas clumps located at the center of G10.6-0.4 and

  11. Nanoparticle Delivered Human Biliverdin Reductase-Based Peptide Increases Glucose Uptake by Activating IRK/Akt/GSK3 Axis: The Peptide Is Effective in the Cell and Wild-Type and Diabetic Ob/Ob Mice

    PubMed Central

    Gibbs, Peter E. M.; Miralem, Tihomir; Lerner-Marmarosh, Nicole; Maines, Mahin D.

    2016-01-01

    Insulin's stimulation of glucose uptake by binding to the IRK extracellular domain is compromised in diabetes. We have recently described an unprecedented approach to stimulating glucose uptake. KYCCSRK (P2) peptide, corresponding to the C-terminal segment of hBVR, was effective in binding to and inducing conformational change in the IRK intracellular kinase domain. Although myristoylated P2, made of L-amino acids, was effective in cell culture, its use for animal studies was unsuitable. We developed a peptidase-resistant formulation of the peptide that was efficient in both mice and cell culture systems. The peptide was constructed of D-amino acids, in reverse order, and blocked at both termini. Delivery of the encapsulated peptide to HepG2 and HSKM cells was confirmed by its prolonged effect on stimulation of glucose uptake (>6 h). The peptide improved glucose clearance in both wild-type and Ob/Ob mice; it lowered blood glucose levels and suppressed glucose-stimulated insulin secretion. IRK activity was stimulated in the liver of treated mice and in cultured cells. The peptide potentiated function of IRK's downstream effector, Akt-GSK3-(α, β) axis. Thus, P2-based approach can be used for improving glucose uptake by cells. Also, it allows for screening peptides in vitro and in animal models for treatment of diabetes. PMID:27294151

  12. Direct in vitro effects of androgens and estrogens on ob gene expression and leptin secretion in human adipose tissue.

    PubMed

    Machinal-Quélin, Florence; Dieudonné, Marie-Noëlle; Pecquery, René; Leneveu, Marie-Christine; Giudicelli, Yves

    2002-07-01

    In the present study, we have explored, in vitro, the possibility that short exposure to androgens and estrogens for 24 h may directly influence leptin expression (ARNm and secretion) in sc adipose tissue from men and women. In men, only dihydrotestosterone at high concentration (100 nM) induced a reduction in leptin secretion and ob mRNA level. In women, 17beta-estradiol (10-100 nM) increased ob mRNA expression (+180 to +500%) and leptin release (+75%). Moreover, in adipose tissue of women, the estrogen precursors testosterone (100 nM) and dehydroepiandrosterone (1 microM) also induced an increase in leptin secretion (+84 and +96%, respectively), an effect that was prevented by the aromatase inhibitor letrozole. Finally, the stimulatory effect of 17beta-estradiol observed in women was antagonized by the antiestrogen ICI182780. Altogether, these results suggest that the sexual dimorphism of leptinemia in humans is mainly owing to the estrogen receptor-dependent stimulation of leptin expression in adipose tissue by estrogens and estrogen precursors in women. PMID:12374466

  13. Temperature-dependent Raman spectroscopic study of bismuth borate Bi2ZnOB2O6

    NASA Astrophysics Data System (ADS)

    Zhang, Ji; Zhang, De-Ming; Zhang, Qing-Li; Yin, Shao-Tang

    2015-01-01

    A temperature-dependent Raman spectroscopic study on Bi2ZnOB2O6 crystal was carried out to investigate the structure change of the crystal with the increase of temperature. Raman spectra of crystal Bi2ZnOB2O6 were recorded in the spectral range 10-1600 cm-1 at room temperature first. Compared with the vibrational spectra of the referred compounds, satisfactory assignment of most of the high-energy modes to vibrations of Bi-O, B-O, and Zn-O bonds was achieved. In particular, the Raman high-frequency peak located at 1344 cm-1 was attributed to the B-O vibration in the BO3 triangle. This temperature-dependent Raman spectroscopic study was carried out up to 600 °C. It was found that all the Raman lines exhibit decreases in frequency and the widths of the Raman peaks increase with increasing temperature. No phase transition was observed under 600 °C. Project supported by the National Natural Science Foundation of China (Grant Nos. 50932005 and 51102239).

  14. Multifunctional Bi{sub 2}ZnOB{sub 2}O{sub 6} single crystals for second and third order nonlinear optical applications

    SciTech Connect

    Iliopoulos, K.; Kasprowicz, D.; Majchrowski, A.; Michalski, E.; Gindre, D.; Sahraoui, B.

    2013-12-02

    Bi{sub 2}ZnOB{sub 2}O{sub 6} nonlinear optical single crystals were grown by means of the Kyropoulos method from stoichiometric melt. The second and third harmonic generation (SHG/THG) of Bi{sub 2}ZnOB{sub 2}O{sub 6} crystals were investigated by the SHG/THG Maker fringes technique. Moreover, SHG microscopy studies were carried out providing two-dimensional SHG images as a function of the incident laser polarization. The high nonlinear optical efficiency combined with the possibility to grow high quality crystals make Bi{sub 2}ZnOB{sub 2}O{sub 6} an excellent candidate for photonic applications.

  15. Noise on broadband Ocean Bottom Seismometers (OBS) from the German (DEPAS) and French (INSU) instruments pools as recorded in the RHUM-RUM project

    NASA Astrophysics Data System (ADS)

    Stähler, Simon C.; Sigloch, Karin; Barruol, Guilhem; Hosseini, Kasra; Crawford, Wayne C.; Schmidt-Aursch, Mechita; Tsekhmistrenko, Maria; Scholz, John-Robert; Deen, Martha; Mazzullo, Alessandro

    2016-04-01

    A long-standing discussion in the OBS community is about the influence of OBS design on noise levels of seismic records. We present results from the RHUM-RUM experiment in the Indian Ocean. RHUM-RUM is a German-French seismological experiment based on the sea floor surrounding the island of La Réunion, western Indian Ocean. RHUM-RUM's central component is a 13-month deployment (Oct 2012 to Nov 2013) of 57 broad- and wideband ocean bottom seismometers (OBS) and hydrophones over an area of 2000x2000 km2 surrounding the hotspot. The array contained 48 wideband OBS from the German DEPAS pool and 9 broadband OBS from the French INSU pool. It is the largest deployment of DEPAS and INSU OBS so far, and the first joint experiment. Therefore it allows to compare the performance of these distinct instrument types in different ocean-floor environments. The INSU seismic sensors stand away from their OBS frames, whereas the DEPAS sensors are integrated into theirs. At long periods (>10 s), the DEPAS seismometers are affected by significantly stronger noise than the INSU seismometers. On the horizontal components, this can be explained by tilting of the frame and buoy assemblage, e.g., through the action of ocean-bottom currents. However, the long period noise level on the vertical components suggests that the DEPAS intruments are also affected by significant self-noise of the CMG-40TOBS seismometer itself. By comparison, the INSU instruments (Trillium 240OBS sensors) are much quieter at periods >30 s and hence better suited for long-period studies. The trade-off of the instrument design is that the integrated DEPAS setup is easier to deploy and recover, especially when large numbers of stations are involved or fast deployment/recovery of the instruments is desired (e.g. active experiments). Additionally, the wideband sensor has only half the power consumption of the broadband INSU seismometers. This presentation also reviews network performance and data quality: Of the 57

  16. Relationship of W-R star numbers and subclass distribution to OB star numbers and metallicity in galaxies

    SciTech Connect

    Smith, L.F.

    1988-04-01

    The population of W-R stars in local group galaxies are compared on the basis of quantities expected to be little affectrd by incompleteness of the surveys. The ratio of the number of strong line W-R stars to the number of OB stars with M(V) less than -4.5 is found to range from less than 1/1000 to more than 1/10 and correlates with metallicity. The low-metallicity galaxies have lower numbers of W-R stars, and all of these may be binaries. The ratio between number of stars in the two sequences WC/WNE (single) also correlates with metallicity. The presence of later subclasses of WC stars is strongly correlated with metallicity; WC 7-9 stars occur only in the inner part of M33 and in the metal-rich galaxies MWG and M31. 81 references.

  17. TOWARD COMPLETE STATISTICS OF MASSIVE BINARY STARS: PENULTIMATE RESULTS FROM THE CYGNUS OB2 RADIAL VELOCITY SURVEY

    SciTech Connect

    Kobulnicky, Henry A.; Lundquist, Michael J.; Burke, Jamison; Chapman, James; Keller, Erica; Lester, Kathryn; Rolen, Emily K.; Topel, Eric; Bhattacharjee, Anirban; Smullen, Rachel A.; Álvarez, Carlos A. Vargas; Runnoe, Jessie C.; Dale, Daniel A.; Brotherton, Michael M.; Kiminki, Daniel C. E-mail: jburke2@swarthmore.edu E-mail: kelle22e@mtholyoke.edu E-mail: emily.k.rolen@vanderbilt.edu

    2014-08-01

    We analyze orbital solutions for 48 massive multiple-star systems in the Cygnus OB2 association, 23 of which are newly presented here, to find that the observed distribution of orbital periods is approximately uniform in log P for P < 45 days, but it is not scale-free. Inflections in the cumulative distribution near 6 days, 14 days, and 45 days suggest key physical scales of ≅0.2, ≅0.4, and ≅1 A.U. where yet-to-be-identified phenomena create distinct features. No single power law provides a statistically compelling prescription, but if features are ignored, a power law with exponent β ≅ –0.22 provides a crude approximation over P = 1.4-2000 days, as does a piece-wise linear function with a break near 45 days. The cumulative period distribution flattens at P > 45 days, even after correction for completeness, indicating either a lower binary fraction or a shift toward low-mass companions. A high degree of similarity (91% likelihood) between the Cyg OB2 period distribution and that of other surveys suggests that the binary properties at P ≲ 25 days are determined by local physics of disk/clump fragmentation and are relatively insensitive to environmental and evolutionary factors. Fully 30% of the unbiased parent sample is a binary with period P < 45 days. Completeness corrections imply a binary fraction near 55% for P < 5000 days. The observed distribution of mass ratios 0.2 < q < 1 is consistent with uniform, while the observed distribution of eccentricities 0.1 < e < 0.6 is consistent with uniform plus an excess of e ≅ 0 systems. We identify six stars, all supergiants, that exhibit aperiodic velocity variations of ∼30 km s{sup –1} attributed to atmospheric fluctuations.

  18. The 2.35 year itch of Cygnus OB2 #9. I. Optical and X-ray monitoring

    NASA Astrophysics Data System (ADS)

    Nazé, Y.; Mahy, L.; Damerdji, Y.; Kobulnicky, H. A.; Pittard, J. M.; Parkin, E. R.; Absil, O.; Blomme, R.

    2012-10-01

    Context. Nonthermal radio emission in massive stars is expected to arise in wind-wind collisions occurring inside a binary system. One such case, the O-type star Cyg OB2 #9, was proven to be a binary only four years ago, but the orbital parameters remained uncertain. The periastron passage of 2011 was the first one to be observable under good conditions since the discovery of binarity. Aims: In this context, we have organized a large monitoring campaign to refine the orbital solution and to study the wind-wind collision. Methods: This paper presents the analysis of optical spectroscopic data, as well as of a dedicated X-ray monitoring performed with Swift and XMM-Newton. Results: In light of our refined orbital solution, Cyg OB2 #9 appears as a massive O+O binary with a long period and high eccentricity; its components (O5-5.5I for the primary and O3-4III for the secondary) have similar masses and similar luminosities. The new data also provide the first evidence that a wind-wind collision is present in the system. In the optical domain, the broad Hα line varies, displaying enhanced absorption and emission components at periastron. X-ray observations yield the unambiguous signature of an adiabatic collision, because as the stars approach periastron, the X-ray luminosity closely follows the 1/D variation expected in that case. The X-ray spectrum appears, however, slightly softer at periastron, which is probably related to winds colliding at slightly lower speeds at that time. Conclusions: It is the first time that such a variation has been detected in O+O systems, and the first case where the wind-wind collision is found to remain adiabatic even at periastron passage. Based on observations collected at OHP, with Swift, and with XMM-Newton.Tables 1 and 2 are available in electronic form at http://www.aanda.org

  19. NADH-regulated metabolic model for growth of Methylosinus trichosporium OB3b. Model presentation, parameter estimation, and model validation

    SciTech Connect

    Sipkema, E.M.; Koning, W. de; Ganzeveld, K.J.; Janssen, D.B.; Beenackers, A.A.C.M.

    2000-04-01

    Chlorinated ethenes, such as the environmental pollutant trichloroethene, can be converted aerobically only via cometabolism. In this process, conversion results from the nonspecificity of oxygenating enzymes. Expression of these enzymes occurs during growth on compounds such as alkanes, aromatics, and ammonia. A biochemical model is presented that described growth of Methylosinus trichosporium OB3b on methane. The model, which was developed to compare strategies to alleviate NADH limitation resulting from cometabolic contaminant conversion, includes (1) catabolism of methane via methanol, formaldehyde, and formate to carbon dioxide; (2) growth as formaldehyde assimilation; and (3) storage material (poly-{beta}-hydroxy-butyric acid, PHB) metabolism. To integrate the three processes, the cofactor NADH is used as central intermediate and controlling factor--instead of the commonly applied energy carrier ATP. This way a stable and well-regulated growth model is obtained that gives a realistic description of a variety of steady-state and transient-state experimental data. An analysis of the cells' physiological properties is given to illustrate the applicability of the model. Steady-state model calculations showed that in strain OB3b flux control is located primarily at the first enzyme of the metabolic pathway. Since no adaptation in V{sub MAX} values is necessary to describe growth at different dilution rates, the organism seems to have a ``rigid enzyme system,'' the activity of which is not regulated in response to continued growth at low rates. During transient periods of excess carbon and energy source availability, PHB is found to accumulate, serving as a sink for transiently available excess reducing power.

  20. Analyses of Candida Cdc13 Orthologues Revealed a Novel OB Fold Dimer Arrangement, Dimerization-Assisted DNA Binding, and Substantial Structural Differences between Cdc13 and RPA70

    PubMed Central

    Yu, Eun Young; Sun, Jia

    2012-01-01

    The budding yeast Cdc13-Stn1-Ten1 complex is crucial for telomere protection and has been proposed to resemble the RPA complex structurally and functionally. The Cdc13 homologues in Candida species are unusually small and lack two conserved domains previously implicated in telomere regulation, thus raising interesting questions concerning the mechanisms and evolution of these proteins. In this report, we show that the unusually small Cdc13 homologue in Candida albicans is indeed a regulator of telomere lengths and that it associates with telomere DNA in vivo. We demonstrated high-affinity telomere DNA binding by C. tropicalis Cdc13 (CtCdc13) and found that dimerization of this protein through its OB4 domain is important for high-affinity DNA binding. Interestingly, CtCdc13-DNA complex formation appears to involve primarily recognition of multiple copies of a six-nucleotide element (GGATGT) that is shared by many Candida telomere repeats. We also determined the crystal structure of the OB4 domain of C. glabrata Cdc13, which revealed a novel mechanism of OB fold dimerization. The structure also exhibits marked differences to the C-terminal OB fold of RPA70, thus arguing against a close evolutionary kinship between these two proteins. Our findings provide new insights on the mechanisms and evolution of a critical telomere end binding protein. PMID:22025677

  1. Hypothyroidism reduces ObRb-STAT3 leptin signalling in the hypothalamus and pituitary of rats associated with resistance to leptin acute anorectic action.

    PubMed

    Calvino, Camila; Souza, Luana L; Costa-e-Sousa, Ricardo H; Almeida, Norma A S; Trevenzoli, Isis H; Pazos-Moura, Carmen C

    2012-10-01

    Leptin has been shown to regulate the hypothalamus-pituitary-thyroid axis, acting primarily through the STAT3 pathway triggered through the binding of leptin to the long-chain isoform of the leptin receptor, ObRb. We previously demonstrated that although hyperthyroid rats presented leptin effects on TSH secretion, those effects were abolished in hypothyroid rats. We addressed the hypothesis that changes in the STAT3 pathway might explain the lack of TSH response to leptin in hypothyroidism by evaluating the protein content of components of leptin signalling via the STAT3 pathway in the hypothalamus and pituitary of hypothyroid (0·03% methimazole in the drinking water/21 days) and hyperthyroid (thyroxine 5 μg/100 g body weight /5 days) rats. Hypothyroid rats exhibited decreased ObRb and phosphorylated STAT3 (pSTAT3) protein in the hypothalamus, and in the pituitary gland they exhibited decreased ObRb, total STAT3, pSTAT3 and SOCS3 (P<0·05). Except for a modest decrease in pituitary STAT3, no other alterations were observed in hyperthyroid rats. Moreover, unlike euthyroid rats, the hypothyroid rats did not exhibit a reduction in food ingestion after a single injection of leptin (0·5 mg/kg body weight). Therefore, hypothyroidism decreased ObRb-STAT3 signalling in the hypothalamus and pituitary gland, which likely contributes to the loss of leptin action on food intake and TSH secretion, as previously observed in hypothyroid rats. PMID:22875962

  2. Influence of the Verb Prefix "o-/ob-" on the Distribution of Cases on the Nominal Objects: The Occurrence of Transitive Usage of Unprefixed Verbs.

    ERIC Educational Resources Information Center

    Launer, Michael K.

    1986-01-01

    Investigates the influence of the prefix "o-/ob-" on the choice of case for nominal objects of prefixed verbs, using a semantic field analysis. Focuses on four semantic functions: (1) objective; (2) locative; (3) factitive; and (4) comparative. The results are useful both to theoretical linguists and to teachers of Russian. (LMO)

  3. A high angular resolution survey of massive stars in Cygnus OB2: Results from the Hubble space telescope fine guidance sensors

    SciTech Connect

    Caballero-Nieves, S. M.; Gies, D. R.; Jao, W.-C. E-mail: gies@chara.gsu.edu; and others

    2014-02-01

    We present results of a high angular resolution survey of massive OB stars in the Cygnus OB2 association that we conducted with the fine guidance sensor 1R (FGS1r) on the Hubble Space Telescope. FGS1r is able to resolve binary systems with a magnitude difference ΔV < 4 down to separations as small as 0.''01. The sample includes 58 of the brighter members of Cyg OB2, one of the closest examples of an environment containing a large number of very young and massive stars. We resolved binary companions for 12 targets and confirmed the triple nature of one other target, and we offer evidence of marginally resolved companions for two additional stars. We confirm the binary nature of 11 of these systems from complementary adaptive optics imaging observations. The overall binary frequency in our study is 22% to 26% corresponding to orbital periods ranging from 20 to 20,000 yr. When combined with the known short-period spectroscopic binaries, the results support the hypothesis that the binary fraction among massive stars is >60%. One of the new discoveries is a companion to the hypergiant star MT 304 = Cyg OB2-12, and future measurements of orbital motion should provide mass estimates for this very luminous star.

  4. Biogeochemistry of suspended and sedimentary material in the Ob and Yenisei rivers and Kara Sea: amino acids and amino sugars

    NASA Astrophysics Data System (ADS)

    Unger, Daniela; Gaye-Haake, Birgit; Neumann, Kirsten; Catalina Gebhardt, A.; Ittekkot, Venugopalan

    2005-03-01

    Suspended particulate matter (SPM) and sediments were sampled in the Ob and the Yenisei rivers and the adjacent Kara Sea up to 77°N during the ice-free period in August and September 1997 (only sediments), 1999 and 2000. The material was analyzed for organic carbon (OC) and nitrogen (N) as well as for the content and monomeric composition of the labile organic constituents amino acids (AA) and amino sugars (AS). Despite interannual variation, surface SPM concentration generally revealed decreasing values from 8 to 4 mg/L for the Ob and Yenisei to values <0.5 mg/L in the offshore region reflecting the importance of fluvial input for SPM distribution. The quality of surface SPM was mainly determined by planktonic productivity as revealed by C/N, the content of AA+AS and their contribution to OC and N. Sedimentation caused a qualitative change of surface SPM at ˜ 73.5°N with POC-% averaging 6.7% south and 21% north of this latitude. This was accompanied by a change from 12-90 to 105-202 mg AA+AS/g SPM, respectively. Together with the monomeric composition of AA this indicates a higher reactivity of OM and a reduced dilution by detrital material in the offshore region. Whereas SPM was homogenously distributed in the rivers, the estuaries were characterized by a salt-wedge at depth. Deep water revealed highest SPM concentrations due to sediment resuspension and/or the presence of a nepheloid layer, which is mirrored in distinctively reduced OM content and reactivity relative to upper water column SPM. Compared to SPM, sediments are characterized by elevated contents of terrestrial OM in the southern study area especially off the Yenisei. OM content and reactivity decrease in offshore direction due to lower sedimentation rates and terrestrial OM input towards the north. The AA-derived reactivity index (RI) and degradation index (DI) together sensitively reflect the degradational stage of OM in SPM and sediments allowing a precise classification from fresh plankton

  5. CHARA/MIRC observations of two M supergiants in Perseus OB1: Temperature, bayesian modeling, and compressed sensing imaging

    SciTech Connect

    Baron, F.; Monnier, J. D.; Anderson, M.; Aarnio, A.; Kiss, L. L.; Neilson, H. R.; Zhao, M.; Pedretti, E.; Thureau, N.; Ten Brummelaar, T. A.; Sturmann, J.; Sturmann, L.; Turner, N.; Ridgway, S. T.; McAlister, H. A.

    2014-04-10

    Two red supergiants (RSGs) of the Per OB1 association, RS Per and T Per, have been observed in the H band using the Michigan Infra-Red Combiner (MIRC) instrument at the CHARA array. The data show clear evidence of a departure from circular symmetry. We present here new techniques specially developed to analyze such cases, based on state-of-the-art statistical frameworks. The stellar surfaces are first modeled as limb-darkened disks based on SATLAS models that fit both MIRC interferometric data and publicly available spectrophotometric data. Bayesian model selection is then used to determine the most probable number of spots. The effective surface temperatures are also determined and give further support to the recently derived hotter temperature scales of RSGs. The stellar surfaces are reconstructed by our model-independent imaging code SQUEEZE, making use of its novel regularizer based on Compressed Sensing theory. We find excellent agreement between the model-selection results and the reconstructions. Our results provide evidence for the presence of near-infrared spots representing about 3%-5% of the stellar flux.

  6. The far-ultraviolet dust albedo in the Upper Scorpius subgroup of the Scorpius OB2 association

    NASA Astrophysics Data System (ADS)

    Gordon, Karl D.; Witt, Adolf N.; Carruthers, George R.; Christensen, Susan A.; Dohne, Brian C.

    1994-09-01

    During NRL's Far Ultraviolet Cameras experiment on STS-39, four images of the giant reflection nebula encompassing the Upper Scorpius subgroup of the Sco OB2 association were obtained in two ultraviolet bandpasses with lambdaeff = 1362 A and 1769 A. From these images and IUE and TD-1 stellar spectra, the ratio of nebular to stellar flux was calculated. The ratio ranged from 0.577 to 0.921 at 1362 A and 0.681 to 0.916 at 1769 A with the spread in the ratio arising mainly from uncertainties in the sky background. In order to analyze these images, a model utilizing Monte Carlo techniques to describe radiative transfer in a spherical nebula with asymmetrically distributed stars was developed by elaborating on previous work by Witt. This model was used to determine the range of albedos reproducing the observed nebular-to-stellar flux ratios while allowing the scattering phase function asymmetry to vary between 0.0 and 0.8. The resulting albedos were 0.47-0.70 at 1362 A and 0.55-0.72 at 1769 A.

  7. Isolation, characterization, and biological activity of ferredoxin-NAD+ reductase from the methane oxidizer Methylosinus trichosporium OB3b.

    PubMed Central

    Chen, Y P; Yoch, D C

    1989-01-01

    A ferredoxin-NAD+ oxidoreductase (EC 1.18.1.3) has been isolated from extracts of the obligate methanotroph Methylosinus trichosporium OB3b. This enzyme was shown to couple electron flow from formate dehydrogenase (NAD+ requiring) to ferredoxin. Ferredoxin-NAD+ reductase was purified to homogeneity by conventional chromatography techniques and was shown to be a flavoprotein with a molecular weight of 36,000 +/- 1,000. This ferredoxin reductase was specific for NADH (Km, 125 microM) and coupled electron flow to the native ferredoxin and to ferredoxins from spinach, Clostridium pasteurianum, and Rhodospirillum rubrum (ferredoxin II). M. trichosporium ferredoxin saturated the ferredoxin-NAD+ reductase at a concentration 2 orders of magnitude lower (3 nM) than did spinach ferredoxin (0.4 microM). Ferredoxin-NAD+ reductase also had transhydrogenase activity which transferred electrons and protons from NADH to thionicotinamide adenine dinucleotide phosphate (Km, 9 microM) and from NADPH to 3-acetylpyridine adenine dinucleotide (Km, 16 microM). Reconstitution of a soluble electron transport pathway that coupled formate oxidation to ferredoxin reduction required formate dehydrogenase, NAD+, and ferredoxin-NAD+ reductase. Images PMID:2768195

  8. X-ray and IR Surveys of the Orion Molecular Clouds and the Cepheus OB3b Cluster

    NASA Astrophysics Data System (ADS)

    Megeath, S. Thomas; Wolk, Scott J.; Pillitteri, Ignazio; Allen, Tom

    2014-08-01

    X-ray and IR surveys of molecular clouds between 400 and 700 pc provide complementary means to map the spatial distribution of young low mass stars associated with the clouds. We overview an XMM survey of the Orion Molecular Clouds, at a distance of 400 pc. By using the fraction of X-ray sources with disks as a proxy for age, this survey has revealed three older clusters rich in diskless X-ray sources. Two are smaller clusters found at the northern and southern edges of the Orion A molecular cloud. The third cluster surrounds the O-star Iota Ori (the point of Orion's sword) and is in the foreground to the Orion molecular cloud. In addition, we present a Chandra and Spitzer survey of the Cep OB3b cluster at 700 pc. These data show a spatially variable disk fraction indicative of age variations within the cluster. We discuss the implication of these results for understanding the spread of ages in young clusters and the star formation histories of molecular clouds.

  9. Placenta-Specific Protein 1: A Potential Key to Many Oncofetal-Placental OB/GYN Research Questions

    PubMed Central

    Devor, Eric J.; Reyes, Henry D.; Santillan, Donna A.; Santillan, Mark K.; Onukwugha, Chinenye; Goodheart, Michael J.; Leslie, Kimberly K.

    2014-01-01

    Placenta-specific protein 1 (PLAC1) is a secreted protein found in trophoblasts. Several reports implicate a central role for PLAC1 in establishment and maintenance of the placenta. In addition to placentae PLAC1 is expressed in a variety of solids including breast, endometrial, and ovarian cancers. In order to show that PLAC1 is potentially relevant to a number of research questions in OB/GYN, we report on PLAC1 expression in a selected panel that includes two choriocarcinoma cell lines, normal placental tissues, and endometrial and ovarian tumors. We report for the first time that PLAC1 is also expressed in human fetal tissues. PLAC1 is transcriptionally heterogeneous with one promoter (P1) generating two transcripts with alternately spliced 5' UTRs and the other promoter (P2) generating a third transcript. Placental tissues favor P2 transcripts, while P1 is favored in most of the other cells. Mechanisms determining multiple PLAC1 transcripts and promoter preferences are as yet unknown, but it is clear that this protein is likely to be important in a variety of phenomena relevant to both gynecologic oncology and maternal-fetal medicine. PMID:24757447

  10. CHANDRA DETECTS THE RARE OXYGEN-TYPE WOLF-RAYET STAR WR 142 AND OB STARS IN BERKELEY 87

    SciTech Connect

    Sokal, Kimberly R.; Skinner, Stephen L.; Zhekov, Svetozar A.; Guedel, Manuel; Schmutz, Werner E-mail: stephen.skinner@colorado.ed

    2010-06-01

    We present first results of a Chandra X-ray observation of the rare oxygen-type Wolf-Rayet (WR) star WR 142 (= Sand 5 = St 3) harbored in the young, heavily obscured cluster Berkeley 87. Oxygen-type WO stars are thought to be the most evolved of the WRs and progenitors of supernovae or gamma-ray bursts. As part of an X-ray survey of supposedly single WR stars, we observed WR 142 and the surrounding Berkeley 87 region with Chandra ACIS-I. We detect WR 142 as a faint yet extremely hard X-ray source. Due to weak emission, its nature as a thermal or non-thermal emitter is unclear and thus we discuss several emission mechanisms. Additionally, we report seven detections and eight non-detections by Chandra of massive OB stars in Berkeley 87, two of which are bright yet soft X-ray sources whose spectra provide a dramatic contrast to the hard emission from WR 142.

  11. Star formation in Carina OB1: Observations of a giant molecular cloud associated with the eta Carinae Nebula

    NASA Technical Reports Server (NTRS)

    Grabelsky, D. A.; Cohen, R. S.; Thaddeus, P.

    1987-01-01

    A giant molecular cloud associated with the eta Carinae nebula was fully mapped in CO with the Columbia Millimeter-Wave Telescope at Cerro Tololo. The cloud comples has a mass of roughly 700,000 solar mass and extends about 140 pc along the Galactic plane, with the giant Carina HII region situated at one end of the complex. Clear evidence of interaction between the HII region and the molecular cloud is found in the relative motions of the ionized gas, the molecular gas, and the dust; simple energy and momentum considerations suggest that the HII region is responsible for the observed motion of a cloud fragment. The molecular cloud complex appears to be the parent material of the entire Car OB1 Association which, in addition to the young clusters in the Carine nebula, includes the generally older cluster NGC 3325, NGC 3293, and IC 2581. The overall star formation efficiency in the cloud complex is estimated to be approximately 0.02.

  12. Acute kernicterus in a neonate with O/B blood group incompatibility and a mutation in SLC4A1.

    PubMed

    Christensen, Robert D; Yaish, Hassan M; Nussenzveig, Roberto H; Reading, N Scott; Agarwal, Archana M; Eggert, Larry D; Prchal, Josef T

    2013-08-01

    We cared for a term female newborn, who at 108 hours of age, with a total serum bilirubin of 15.4 mg/dL, was discharged from the hospital on home phototherapy. At a return appointment 44 hours later, her total serum bilirubin was 41.7 mg/dL and signs of acute kernicterus were present. Maternal/fetal blood group O/B incompatibility was identified, with a negative direct antiglobulin test, which was positive on retesting. She had abundant spherocytes on blood smear, and these persisted at follow-up, but neither parent had spherocytes identified. A heterozygous SLC4A1(E508K) mutation (gene encoding erythrocyte membrane protein band 3) was found, and in silico predicted to result in damaged erythrocyte cytoskeletal protein function. No mutations were identified in other red cell cytoskeleton genes (ANK1, SPTA1, SPTB, EPB41, EPB42) and the UGT1A1 promoter region was normal. Neurologic follow-up at 2 and 4 months showed developmental delays consistent with mild kernicterus. PMID:23878048

  13. OB CITY-Definition of a Family-Based Intervention for Childhood Obesity Supported by Information and Communication Technologies.

    PubMed

    Hu, Ruofei; Cancela, Jorge; Arredondo Waldmeyer, Maria Teresa; Cea, Gloria; Vlachopapadopoulou, Elpis-Athina; Fotiadis, Dimitrios I; Fico, Giuseppe

    2016-01-01

    Childhood obesity is becoming one of the 21st century's most important public health problems. Nowadays, the main treatment of childhood obesity is behavior intervention that aims at improve children's lifestyle to arrest the disease. Information and communication technologies (ICTs) have not been widely employed in this intervention, and most of existing ICTs systems are not having a long-term effect. The purpose of this paper is to define a system to support family-based intervention through a state-of-the-art analysis of family-based interventions and related technological solutions first, and then using the analytic hierarchy process to derive a childhood obesity family-based behavior intervention model, and finally to provide a prototype of a system called OB CITY. The system makes use of applied behavior analysis, affective computing technologies, as well as serious game and gamification techniques, to offer long term services in all care dimensions of the family-based behavioral intervention aiming to provide positive effects to the treatment of childhood obesity. PMID:27602306

  14. OB CITY–Definition of a Family-Based Intervention for Childhood Obesity Supported by Information and Communication Technologies

    PubMed Central

    Hu, Ruofei; Cancela, Jorge; Cea, Gloria; Vlachopapadopoulou, Elpis-Athina; Fotiadis, Dimitrios I.; Fico, Giuseppe

    2016-01-01

    Childhood obesity is becoming one of the 21st century’s most important public health problems. Nowadays, the main treatment of childhood obesity is behavior intervention that aims at improve children’s lifestyle to arrest the disease. Information and communication technologies (ICTs) have not been widely employed in this intervention, and most of existing ICTs systems are not having a long-term effect. The purpose of this paper is to define a system to support family-based intervention through a state-of-the-art analysis of family-based interventions and related technological solutions first, and then using the analytic hierarchy process to derive a childhood obesity family-based behavior intervention model, and finally to provide a prototype of a system called OB CITY. The system makes use of applied behavior analysis, affective computing technologies, as well as serious game and gamification techniques, to offer long term services in all care dimensions of the family-based behavioral intervention aiming to provide positive effects to the treatment of childhood obesity. PMID:27602306

  15. Global Molecular Analyses of Methane Metabolism in Methanotrophic Alphaproteobacterium, Methylosinus trichosporium OB3b. Part I: Transcriptomic Study

    PubMed Central

    Matsen, Janet B.; Yang, Song; Stein, Lisa Y.; Beck, David; Kalyuzhnaya, Marina G.

    2013-01-01

    Methane utilizing bacteria (methanotrophs) are important in both environmental and biotechnological applications, due to their ability to convert methane to multicarbon compounds. However, systems-level studies of methane metabolism have not been carried out in methanotrophs. In this work we have integrated genomic and transcriptomic information to provide an overview of central metabolic pathways for methane utilization in Methylosinus trichosporium OB3b, a model alphaproteobacterial methanotroph. Particulate methane monooxygenase, PQQ-dependent methanol dehydrogenase, the H4MPT-pathway, and NAD-dependent formate dehydrogenase are involved in methane oxidation to CO2. All genes essential for operation of the serine cycle, the ethylmalonyl-CoA (EMC) pathway, and the citric acid (TCA) cycle were expressed. PEP-pyruvate-oxaloacetate interconversions may have a function in regulation and balancing carbon between the serine cycle and the EMC pathway. A set of transaminases may contribute to carbon partitioning between the pathways. Metabolic pathways for acquisition and/or assimilation of nitrogen and iron are discussed. PMID:23565111

  16. A Hectochelle Radial Velocity Survey of Cep OB3b: An ONC like cluster at late gas dispersal phase

    NASA Astrophysics Data System (ADS)

    Karnath, Nicole; Allen, Thomas; Prchlik, Jakub; Gutermuth, Robert A.; Megeath, Samuel Thomas; Pipher, Judith; Wolk, Scott J.

    2016-01-01

    Cep OB3b is a young (~3-5 Myr), late gas dispersal cluster of roughly 3000 members broken into two sub-clusters (Eastern and Western) at a distance of 700pc; it is a rare example of nearby cluster in the late stages of gas dispersal and appears to be a more evolved analog to the Orion Nebular Cluster. As part of an ongoing multi wavelength study, we focus on Hectochelle data from the MMT to measure the radial velocities of 499 stars. After removing binaries, outliers, and imposing a minimum R value to the cross correlation, we obtain radial velocities of 57 previously identified members, with an average error of 1.7 km/s. There is no observed variation in radial velocity across the cluster in right ascension or declination. The preferred mechanism for this type of kinematic evolution is that any initial kinematic structure from formation may have been erased and that minimal or no rotation is present in the cluster. However, the Eastern sub-cluster, containing the most massive star in the field, an O7 star, has a higher velocity dispersion than the Western sub-cluster, which contains several B stars. We will compare these results to CO maps of the residual gas in the cluster and discuss possible reasons for this difference. Finally, we will assess whether the cluster is bound or in a state of expansion.

  17. VizieR Online Data Catalog: RIOTS4: spectroscopic survey of SMC field OB stars (Lamb+, 2016)

    NASA Astrophysics Data System (ADS)

    Lamb, J. B.; Oey, M. S.; Segura-Cox, D. M.; Graus, A. S.; Kiminki, D. C.; Golden-Marx, J. B.; Parker, J. W.

    2016-04-01

    Runaways and Isolated O-Type Star Spectroscopic Survey of the SMC (RIOTS4) targets a spatially complete sample of 374 uniformly selected candidate field OB stars in the SMC. Our targets are identified by Oey et al. (2004, J/AJ/127/1632). We observed the RIOTS4 survey targets over a five-year period from 2006 September to 2011 October using spectrographs on the Magellan telescopes at Las Campanas Observatory. The majority of our observations were obtained with the Inamori-Magellan Areal Camera and Spectrograph (IMACS) in the f/4 multi-slit mode on the Magellan Baade telescope (slit widths of either 0.7" or 1.0", yielding spectral resolutions of R~3700 and R~2600, respectively). With IMACS f/4 out of commission during our 2011 observations, we also operated IMACS in f/2 mode (we used either a 0.5" or 0.7" slit width, which yield spectral resolutions of R~1000 and R~1300, respectively). The remaining targets were observed using the Magellan Inamori Kyocera Echelle (MIKE) Spectrograph on the Magellan Clay telescope (R~28000). All MIKE observations occurred in 2010 November. (2 data files).

  18. VizieR Online Data Catalog: RVs & V-band LCs of probable members of Cyg OB2 (Kiminki+, 2015)

    NASA Astrophysics Data System (ADS)

    Kiminki, D. C.; Kobulnicky, H. A.; Vargas Alvarez, C. A.; Alexander, M. J.; Lundquist, M. J.

    2016-01-01

    Optical spectroscopic observations of the four targets were obtained as part of the Cygnus OB2 Radial Velocity Survey on numerous dates between 2001 and 2011 using a variety of telescopes and instruments as described in Papers I-IV (see References section below) of this series. Several additional observations were obtained in 2012 October (on MT91 696 and Schulte 3) and 2013 May-June (on MT91 372) using the Wyoming Infrared Observatory (WIRO). Photometry of MT91 696 was obtained on nine nights between 2012 October 17 and November 10 at the University of Wyoming 0.6m Red Buttes Observatory (RBO). Data on Schulte 3 was obtained on 14 nights at RBO between 2012 October 17 and December 21, and data on CPR2002 A36 was obtained on four nights between 2012 December 10 and 22. Photometry of MT91 372 was also obtained at RBO on seven nights between 2013 June 14 and July 8. (6 data files).

  19. OB3D, a new set of 3D objects available for research: a web-based study

    PubMed Central

    Buffat, Stéphane; Chastres, Véronique; Bichot, Alain; Rider, Delphine; Benmussa, Frédéric; Lorenceau, Jean

    2014-01-01

    Studying object recognition is central to fundamental and clinical research on cognitive functions but suffers from the limitations of the available sets that cannot always be modified and adapted to meet the specific goals of each study. We here present a new set of 3D scans of real objects available on-line as ASCII files, OB3D. These files are lists of dots, each defined by a triplet of spatial coordinates and their normal that allow simple and highly versatile transformations and adaptations. We performed a web-based experiment to evaluate the minimal number of dots required for the denomination and categorization of these objects, thus providing a reference threshold. We further analyze several other variables derived from this data set, such as the correlations with object complexity. This new stimulus set, which was found to activate the Lower Occipital Complex (LOC) in another study, may be of interest for studies of cognitive functions in healthy participants and patients with cognitive impairments, including visual perception, language, memory, etc. PMID:25339920

  20. Offset time decision (OTD) algorithm for guaranteeing the requested QoS of high priority traffic in OBS networks

    NASA Astrophysics Data System (ADS)

    So, Won-Ho; Cha, Yun-Ho; Roh, Sun-Sik; Kim, Young-Chon

    2001-10-01

    In this paper, we propose the Offset Time Decision (OTD) algorithm for supporting the QoS in optical networks based on Optical Burst Switching (OBS), which is the new switching paradigm, and evaluate the performance of the OTD algorithm. The proposed algorithm can decide a reasonable offset time to guarantee the Burst Loss Rate (BLR) of high priority traffic by considering traffic load of network and the number of wavelengths. In order to design this effective OTD algorithm, firstly we illustrate the new burst loss formula, which includes the effect of offset time of high priority class. As the decision of offset time corresponding to the requested BLR, however, should use the reversed formula of new one, we are not able to use it without any changes. Thus, we define the Heuristic Loss Formula (HLF) that is based on the new burst loss formula and the proportional equation considering its characteristics. Finally we show the OTD algorithm to decide the reasonable offset time by using HLF. The simulation result shows that the requested BLR of high priority traffic is guaranteed under various traffic load.

  1. A WIDE-FIELD NARROWBAND OPTICAL SURVEY OF THE BRAID NEBULA STAR FORMATION REGION IN CYGNUS OB7

    SciTech Connect

    Magakian, Tigran Yu.; Nikogossian, Elena H.; Movsessian, Tigran; Aspin, Colin; Pyo, Tae-Soo; Khanzadyan, Tigran; Smith, Michael D.; Mitchison, Sharon; Davis, Chris J.; Beck, Tracy L.; Moriarty-Schieven, Gerald H. E-mail: elena@bao.sci.am E-mail: pyo@subaru.naoj.org E-mail: smm23@kent.ac.uk E-mail: c.davis@jach.hawaii.edu E-mail: gerald.schieven@nrc-cnrc.gc.ca

    2010-03-15

    We study the population of Herbig-Haro (HH) flows and jets in an area of Cygnus OB7 designated the Braid Nebula star formation region. This complex forms part of the L 1003 dark cloud, and hosts two FU Orionis (FUor)-like objects as well as several other active young stars. To trace outflow activity and to relate both known and newly discovered flows to young star hosts we intercompare new, deep, narrowband H{alpha} and [S II] optical images taken on the Subaru 8 m Telescope on Mauna Kea, Hawaii. Our images show that there is considerable outflow and jet activity in this region suggesting the presence of an extensive young star population. We confirm that both of the FUor-like objects drive extensive HH flows and document further members of the flows in both objects. The L 1003 star formation complex is a highly kinematically active region with young stars in several different stages of evolution. We trace collimated outflows from numerous young stars although the origin of some HH objects remains elusive.

  2. The initial mass function for massive stars in the Magellanic Clouds. 1: UBV photometry and color-magnitude diagrams for 14 OB associations

    NASA Technical Reports Server (NTRS)

    Hill, Robert J.; Madore, Barry F.; Freedman, Wendy L.

    1994-01-01

    UBV charge coupled device (CCD) photometry has been obtained for 14 OB associations in the Magellanic Clouds using the University of Toronto's 0.6 m telescope and the Carnegie Institution of Washington's 1.0 m reflector, both on Las Campanas, Chile. The data are presented and used to construct color-magnitude diagrams for the purposes of investigating the massive-star content of the associations.

  3. Shear wave splitting observations across the Juan de Fuca plate system: Ridge- to-trench constraints on mantle flow from 2 years of Cascadia Initiative OBS data

    NASA Astrophysics Data System (ADS)

    Bodmer, M.; Toomey, D. R.; Hooft, E. E. E.

    2014-12-01

    We present SKS splitting measurements for the first two years of data collected by the Cascadia Initiative (CI) amphibious array. Our analysis includes observations from over 100 ocean bottom seismometers (OBS), as well as 31 onshore stations, and spans both the Juan de Fuca and Gorda plates. The CI dataset is unique in that it includes several regions that can distinctly influence anisotropic fabric development such as: the upwelling mantle beneath the Juan de Fuca and Gorda ridges, the young evolving oceanic lithosphere of the plate interior, the Blanco transform fault, and the Cascadia subduction zone. For the first time, we are able to analyze these regions with a single dataset, and using a common methodology. Splitting measurements are routinely done on land sites, but have been completed on relatively few OBS stations. This is largely due to the low signal to noise present in OBS data, which can obscure the splitting results. To address that nearly all the OBS data exceeds the global high noise limit at the frequencies used for splitting, we implement a rigorous quality control scheme. Our method specifically takes into account the response of common splitting methods to high noise data and addresses known issues such as cycle skipping, false minima, low transverse energy, and near-null measurements. Individual measurements are filtered at 0.03-0.1 Hz, manually checked for quality, and stacked. Preliminary results show trench perpendicular onshore measurements consistent with previous studies. Oceanic measurements in the plate interior show a coherent fast axis roughly aligned with absolute plate motion. Several measurements near the ridge and trench appear to be rotated in the ridge and trench parallel directions. Continuing work will integrate splitting measurements from the final two years of the CI with these findings, which will be used to characterize the ridge-to-trench mantle flow across the Juan de Fuca plate system.

  4. High S/N Echelle spectroscopy in young stellar groups. II. Rotational velocities of early-type stars in SCO OB2.

    NASA Astrophysics Data System (ADS)

    Brown, A. G. A.; Verschueren, W.

    1997-03-01

    We investigate the rotational velocities of early-type stars in the Sco OB2 association. We measure v.sin(i) for 156 established and probable members of the association. The measurements are performed with three different techniques, which are in increasing order of expected v.sin(i): 1) converting the widths of spectral lines directly to v.sin(i), 2) comparing artificially broadened spectra of low v.sin(i) stars to the target spectrum, 3) comparing the HeI λ4026 line profile to theoretical models. The sample is extended with literature data for 47 established members of Sco OB2. Analysis of the v.sin(i) distributions shows that there are no significant differences between the subgroups of Sco OB2. We find that members of the binary population of Sco OB2 on the whole rotate more slowly than the single stars. In addition, we find that the B7-B9 single star members rotate significantly faster than their B0-B6 counterparts. We test various hypotheses for the distribution of v.sin(i) in the association. The results show that we cannot clearly exclude any form of random distribution of the direction and/or magnitude of the intrinsic rotational velocity vector. We also investigate the effects of rotation on colours in the Walraven photometric system. We show that positions of B7-B9 single dwarfs above the main sequence are a consequence of rotation. This establishes the influence of rotation on the Walraven colours, due primarily to surface gravity effects.

  5. Carbenoxolone Treatment Ameliorated Metabolic Syndrome in WNIN/Ob Obese Rats, but Induced Severe Fat Loss and Glucose Intolerance in Lean Rats

    PubMed Central

    Prasad Sakamuri, Siva Sankara Vara; Sukapaka, Mahesh; Prathipati, Vijay Kumar; Nemani, Harishankar; Putcha, Uday Kumar; Pothana, Shailaja; Koppala, Swarupa Rani; Ponday, Lakshmi Raj Kumar; Acharya, Vani; Veetill, Giridharan Nappan; Ayyalasomayajula, Vajreswari

    2012-01-01

    Background 11beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) regulates local glucocorticoid action in tissues by catalysing conversion of inactive glucocorticoids to active glucocorticoids. 11β-HSD1 inhibition ameliorates obesity and associated co-morbidities. Here, we tested the effect of 11β-HSD inhibitor, carbenoxolone (CBX) on obesity and associated comorbidities in obese rats of WNIN/Ob strain, a new animal model for genetic obesity. Methodology/Principal Findings Subcutaneous injection of CBX (50 mg/kg body weight) or volume-matched vehicle was given once daily for four weeks to three month-old WNIN/Ob lean and obese rats (n = 6 for each phenotype and for each treatment). Body composition, plasma lipids and hormones were assayed. Hepatic steatosis, adipose tissue morphology, inflammation and fibrosis were also studied. Insulin resistance and glucose intolerance were determined along with tissue glycogen content. Gene expressions were determined in liver and adipose tissue. CBX significantly inhibited 11β-HSD1 activity in liver and adipose tissue of WNIN/Ob lean and obese rats. CBX significantly decreased body fat percentage, hypertriglyceridemia, hypercholesterolemia, insulin resistance in obese rats. CBX ameliorated hepatic steatosis, adipocyte hypertrophy, adipose tissue inflammation and fibrosis in obese rats. Tissue glycogen content was significantly decreased by CBX in liver and adipose tissue of obese rats. Severe fat loss and glucose- intolerance were observed in lean rats after CBX treatment. Conclusions/Significance We conclude that 11β-HSD1 inhibition by CBX decreases obesity and associated co-morbidities in WNIN/Ob obese rats. Our study supports the hypothesis that inhibition of 11β-HSD1 is a key strategy to treat metabolic syndrome. Severe fat loss and glucose -intolerance by CBX treatment in lean rats suggest that chronic 11β-HSD1 inhibition may lead to insulin resistance in normal conditions. PMID:23284633

  6. Methylosinus trichosporium OB3b Mutants Having Constitutive Expression of Soluble Methane Monooxygenase in the Presence of High Levels of Copper

    PubMed Central

    Phelps, Patricia A.; Agarwal, Sandeep K.; Speitel, Gerald E.; Georgiou, George

    1992-01-01

    The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grown in the presence of copper at concentrations typically found in polluted groundwater. Under these conditions, M. trichosporium OB3b expresses a particulate form of the enzyme (pMMO), which has a narrow substrate specificity and does not degrade TCE at any significant rate. We have isolated M. trichosporium OB3b mutants that are deficient in pMMO and express sMMO constitutively in the presence of elevated concentrations of copper. One mutant (PP358) exhibited a TCE degradation rate which was almost twice as high as that of the wild-type strain grown under optimal conditions (without copper). All of the mutants lost the ability to express pMMO activity and to form stacked intracellular membranes characteristic of wild-type cells expressing pMMO. Images PMID:16348810

  7. Enhancing the Quality of Service for Real Time Traffic over Optical Burst Switching (OBS) Networks with Ensuring the Fairness for Other Traffics.

    PubMed

    Al-Shargabi, Mohammed A; Shaikh, Asadullah; Ismail, Abdulsamad S

    2016-01-01

    Optical burst switching (OBS) networks have been attracting much consideration as a promising approach to build the next generation optical Internet. A solution for enhancing the Quality of Service (QoS) for high priority real time traffic over OBS with the fairness among the traffic types is absent in current OBS' QoS schemes. In this paper we present a novel Real Time Quality of Service with Fairness Ratio (RT-QoSFR) scheme that can adapt the burst assembly parameters according to the traffic QoS needs in order to enhance the real time traffic QoS requirements and to ensure the fairness for other traffic. The results show that RT-QoSFR scheme is able to fulfill the real time traffic requirements (end to end delay, and loss rate) ensuring the fairness for other traffics under various conditions such as the type of real time traffic and traffic load. RT-QoSFR can guarantee that the delay of the real time traffic packets does not exceed the maximum packets transfer delay value. Furthermore, it can reduce the real time traffic packets loss, at the same time guarantee the fairness for non real time traffic packets by determining the ratio of real time traffic inside the burst to be 50-60%, 30-40%, and 10-20% for high, normal, and low traffic loads respectively. PMID:27583557

  8. Anatomy of T Phases Recorded by An OBS at 5 km Water Depth: Effects of Local Bathymetry and SOFAR Channel Heterogeneity

    NASA Astrophysics Data System (ADS)

    Chi, W.; Kuo, B.; Tu, T.; Lin, C.; Ando, M.; Lin, C.; Collina, J.

    2008-12-01

    We have deployed broadband ocean bottom seismometers (OBSs) offshore eastern Taiwan in 2006 and 2007. One OBS at 5 km water depth has routinely recorded abnormal seismic phase after P and S phases. The attributes of the energy, including frequency band and arrival time, are consistent with that of a T wave. T waves are phases contained at least some acoustic paths through water bodies. It is still not clear how the T phase energy leaks out of the SOFAR channel before being recorded at depth. From regional physical oceanographic data we found heterogeneous SOFAR channel near the OBS site. Thus, some of the energy can be scattered out of the waveguide. We used an earthquake near the coast to calculate the traveltimes through different solid-earth to acoustic conversion points along the regional 1000 meter bathymetry contour line where the regional SOFAR channel axis is located. The beginning of the T wave usually travel with a path that follows Fermat's principle, instead of the shortest path. The T wave ends when there are no effective conversion points available in the regional bathymetry. The maximum amplitude of the T phase usually has a path with the least solid-earth leg. In addition, T phase amplitude decreases when a typhoon passed between the conversion points and the OBS, possibly due to the strong winds causing mixing of the upper SOFAR channel, making the waveguide less effective.

  9. Identifying Massive Runaway Stars by Detecting Infrared Bowshock Nebula: Four OB Stars and a New Massive Early-B Binary System

    NASA Astrophysics Data System (ADS)

    Sorber, Rebecca L.; Rebecca L. Sorber, Henry A. Kobulnicky, Daniel A. Dale, Matthew S. Povich, William T. Chick, Heather N. Wernke, Julian E. Andrews, Stephan Munari, Grace M. Olivier, Danielle Schurhammer

    2016-01-01

    Though the main sequence evolution of OB type stars is relatively well known, the mass loss rates for these stars are still highly uncertain. Some OB stars are gravitationally ejected from their birth sites, traveling at speeds of 30 km/s or more which results in a prominent bowshock nebulae. We identified OB bowshock candidates at low Galactic latitudes by visual inspection of the Wide-field Infrared Survey Explorer (WISE) 22-micron images. Each candidate was observed using the Longslit Spectrograph at the Wyoming Infrared Observatory (WIRO) 2.3 meter telescope. We present here the results from observing four such candidates, and all four are confirmed as early type stars: GO92.3191+0.0591 (B1V) (aka ALS11826), GO86.551014-1.0873935 (B2V; a probable short-period binary), G076.6921-2.4071 (B5V), and G075.5711-0.2558 (B0V) (aka HD 194303). These results enlarge the sample of candidate runaway massive stars hosting bowshocks and provide a promising sample of such objects for studying stellar mass loss. This work is supported by the National Science Foundation Grants AST-1063146 (REU), AST-1411851 (RUI), and AST-1412845.

  10. Structure of the upper layer of the Kara Sea influenced by Ob and Yenisei discharge based on continuous thermohaline and optical measurements

    NASA Astrophysics Data System (ADS)

    Osadchiev, Alexander; Zavialov, Peter; Izhitskiy, Alexander; Polukhin, Alexander; Pelevin, Vadim; Makkaveev, Petr; Toktamysova, Zhamal

    2015-04-01

    The Kara Sea is significantly affected by continental runoff annually receiving about 1350 km3 of river waters. More than 70% of this volume is discharged from Ob and Yenisei gulfs in June - September and forms a buoyant plume that occupies up to 40% of the Kara Sea area. This work is focused on the structure of the upper layer of the southern part of the Kara Sea in September, 2011 which was dominated by large freshwater discharge. The research is based on the statistical analysis of in situ data collected during the 59th cruise of the R/V "Academician Mstislav Keldysh" along the ship track using a pump-through system (temperature, salinity, pH) and ultraviolet fluorescent lidar (concentrations of chlorophyll, total suspended matter and colored dissolved organic matter) with high space resolution (about 100 m). We performed principal component analysis of the large dataset to identify the areas where the considered water parameters can regard as passive tracers of river discharge. Proximity of discharge volumes of Ob and Yenisei rivers together with difference in their thermohaline and chemical properties relatively big distance between Ob and Yenisei gulfs (about 200 km) result in strong nonuniformity of the freshened plume. The subsequent cluster analysis identified spatial characteristics of different water masses within the studied river plume formed by two sources of freshwater discharge.

  11. [Modeling of Processes of Migration and Accumulation of Radionuclides in Freshwater Ecosystems by the Example of the Samson, Lev, Vandras Rivers Related to the Ob-Irtysh River Basin].

    PubMed

    Trapeznikov, A V; Korzhavin, A V; Trapeznikova, V N; Nikolkin, V N

    2016-01-01

    Mathematical models of horizontal distribution and migration of radionuclides are presented in water and floodplain soils of the Samson-Lev-Vandras river system related to the Ob-Irtysh river basin. Integral inventory of radionuclides in the main components of the river ecosystems is calculated. The estimated annual discharge of radionuclides from the Vandras river to the Great Salym river is given. The effect of the removal of man-made radionuclides in the Samson, Lev, Vandras rivers on radioactive contamination of the Ob-Irtysh river system is shown in comparison with the Techa river, that also belongs to the Ob-Irtysh river basin. Despite the presence of an additional radioactive contamination of the Samson floodplain, the transfer of radioactive substances in the Samson, Lev, Vandras rivers has a much smaller impact on the contamination of the Ob-Irtysh river system, compared to the Techa river, prone to a large-scale radioactive contamination. PMID:27534071

  12. Surpoids et obésité dans la population générale de 5 à 19 ans en milieu urbain bamakois (Mali)

    PubMed Central

    Oumar Bâ, Hamidou; Menta, Ichaka; Camara, Youssouf; Doumbia, Plutôt Seydou; Diarra, Mamadou Bocary

    2014-01-01

    Introduction Déterminer la prévalence du surpoids et de l'obésité dans la population âgée de 5 à 19 ans et fournir des données de référence pour de futures études. Méthodes Notre échantillon est issu de la première étude sur les pathologies cardiovasculaires basée sur l'approche STEP de l'Organisation Mondiale de la Santé (OMS) en sélectionnant tous les sujets âgés de 5 à 19 ans. Nous avons utilisé les méthodes de l'OMS et de l'International Obesity Task Force (IOTF) pour déterminer la prévalence du surpoids et de l'obésité dans la population générale. Résultats La moyenne d’âge était de 11,75 ans ± 4,387 et le sex-ratio M:F était de 0,79. les moyennes pour le poids et la taille étaient de 36,85 kg et 143,48 cm. Selon les critères OMS 1,61% des garçons et 3,28% des filles étaient en surpoids et 0,92% des garçons contre 1,46% des filles obèses. Selon les critères de l'IOTF 4,10% des garçons et 5,94% des filles étaient en surpoids tandis que 0,72% des garçons et 2,68% des filles étaient obèses. Conclusion Malgré sa faible prévalence le surpoids et l'obésité doivent être régulièrement étudiés pour reconnaître des tendances et prendre des mesures adéquates de prévention. Les 2 méthodes utilisées ont permis d'avoir des données de référence pour de futures études au Mali et ailleurs. PMID:25932064

  13. The Herschel/PACS view of the Cep OB2 region: Global protoplanetary disk evolution and clumpy star formation

    NASA Astrophysics Data System (ADS)

    Sicilia-Aguilar, Aurora; Roccatagliata, Veronica; Getman, Konstantin; Rivière-Marichalar, Pablo; Birnstiel, Tilman; Merín, Bruno; Fang, Min; Henning, Thomas; Eiroa, Carlos; Currie, Thayne

    2015-01-01

    Context. The Cep OB2 region, with its two intermediate-aged clusters Tr 37 and NGC 7160, is a paradigm of sequential star formation and an ideal site for studies of protoplanetary disk evolution. Aims: We use Herschel data to study the protoplanetary disks and the star formation history of the region. Methods: Herschel/PACS observations at 70 and 160 μm probe the disk properties (mass, dust sizes, structure) and the evolutionary state of a large number of young stars. Far-IR data also trace the remnant cloud material and small-scale cloud structure. Results: We detect 95 protoplanetary disks at 70 μm, 41 at 160 μm, and obtain upper limits for more than 130 objects. The detection fraction at 70 μm depends on the spectral type (88% for K4 or earlier stars, 17% for M3 or later stars) and on the disk type (~50% for full and pre-transitional disks, ~35% for transitional disks, no low-excess/depleted disks detected). Non-accreting disks are not detected, suggesting significantly lower masses. Accreting transition and pre-transition disks have systematically higher 70 μm excesses than full disks, suggestive of more massive, flared and/or thicker disks. Herschel data also reveal several mini-clusters in Tr 37, which are small, compact structures containing a few young stars surrounded by nebulosity. Conclusions: Far-IR data are an excellent probe of the evolution of disks that are too faint for sub-millimetre observations. We find a strong link between far-IR emission and accretion, and between the inner and outer disk structure. Herschel confirms the dichotomy between accreting and non-accreting transition disks. Accretion is a powerful measure of global disk evolution: substantial mass depletion and global evolution need to occur to shut down accretion in a protoplanetary disk, even if the disk has inner holes. Disks likely follow different evolutionary paths: low disk masses do not imply opening inner holes, and having inner holes does not require low disk masses

  14. Optimization of trichloroethylene degradation using soluble methane monooxygenase of Methylosinus trichosporium OB3b expressed in recombinant bacteria

    SciTech Connect

    Jahng, D.; Kim, C.S.; Wood, T.K.; Hanson, R.S.

    1996-08-05

    By complementing cell-free extracts of Pseudomonas putida F1/pSMMO20 with purified soluble methane monooxygenase (sMMO) components of Methylosinus trichospoirium OB3b, the low cloned-gene sMMO activity in the recombinant strain was found to be due to incomplete activity of the hydroxylase component. To address this incomplete activity, additional sMMO-expressing strains were formed by transferring mmo-containing pSMMO20 and pSMMO50 into various bacterial species including pseudomonads and {alpha}-2 subdivision strains such as methanotrophs, methylotrophs, Agrobacterium tumefaciens A114, and Rhizobium meliloti 102F34 (11 new strains screened); sMMO activity was detected in the last two strains. To increase plasmid segregational stability, the hok/sok locus originally from Escherichia coli plasmid R1 was inserted downstream of the mmo locus of pSMMO20 (resulting in pSMMO40) and found to enhance plasmid stability in P. putida F1 and R. meliloti 102F34 (first report of hok/sok in Rhizobium). To further increase sMMO activity, a modified Whittenbury minimal medium was selected from various minimal and complex media based on trichloroethylene (TCE) degradation and growth rates and was improved by removing the sMMO-inhibiting metal ions [Cu(II), Ni(II), and Zn(II)] and chloramphenicol from the medium and by supplementing with an iron source (3.6 {micro}M of ferrous ammonium sulfate). Using chemostat-grown P. putida F1/pSMMO40, it was found that sMMO activity was higher for cells grown at higher dilution rates. These optimization efforts resulted in a twofold increase in the extent of TCE degradation and more consistent sMMO activity.

  15. NLTE spectral analysis of the sdOB primary of the eclipsing binary system LB 3459 (AA Dor)

    NASA Astrophysics Data System (ADS)

    Rauch, T.

    2000-04-01

    We present a spectral analysis of the sdOB primary star of the binary system LB 3459 based on high-resolution high-S/N optical and UV spectra. The metal abundances are determined by means of state-of-the-art NLTE model atmospheres. We determined Teffw42 and log gw{5.2} within very small error limits. The He (1/125 solar), C (1/265), N (1/33), O (1/12), and Si (1/5) abundances appear strongly depleted while that of Fe and Ni are roughly solar and Mg is strongly enriched by a factor of 6. The spectroscopic distance to LB 3459 is d = 396 pc. The mass of the primary component of LB 3459 is 0.330 M_sun derived from comparisons with theoretical models for sdO stars in the log T_eff - \\log g plane. The mass of the secondary is then 0.066 M_sun derived from the mass function. There remains some disagreement between the radius derived from log g and the above mass, and that derived from analysis of the radial-velocity curve and the eclipse curves. LB 3459 is a close binary system which had experienced a common envelope (CE) phase during its evolution. It fits in the ``low mass case B'' scenario of Iben & Livio (1993) and the secondary is a brown dwarf. The spectroscopically determined rotational velocity of the primary is v_rot = 34 ± 10 km* sec-1. Thus even bound rotation (v_rot = 45.7 km* sec-1) cannot be ruled out. Based on observations collected at the European Southern Observatory, La Silla, Chile (proposals 55.D-0319, 56.C-0165) and on data retrieved from the International Ultraviolet Explorer (IUE) Final Archive.

  16. CONSTRAINTS ON THE MAGELLANIC CLOUDS' INTERACTION FROM THE DISTRIBUTION OF OB STARS AND THE KINEMATICS OF GIANTS

    SciTech Connect

    Casetti-Dinescu, Dana I.; Girard, Terrence M.; Van Altena, William F.; Vieira, Katherine E-mail: terry.girard@yale.edu E-mail: kvieira@cida.ve

    2012-07-10

    Young, OB-type candidates are identified in a {approx}7900 deg{sup 2} region encompassing the Large and Small Magellanic Clouds (LMC/SMC) periphery, the Bridge, part of the Magellanic Stream (MS), and Leading Arm (LA). Selection is based on UV, optical, and IR photometry from existing large-area surveys and proper motions from the Southern Proper Motion 4 (SPM4) catalog. The spatial distribution of these young star candidates shows (1) a well-populated SMC wing which continues westward with two branches partially surrounding the SMC, (2) a rather narrow path from the SMC wing eastward toward the LMC which is offset by 1 Degree-Sign -2 Degree-Sign from the high-density H I ridge in the Bridge, (3) a well-populated periphery of the LMC dominated by clumps of stars at the ends of the LMC bar, and (4) a few scattered candidates in the MS and two overdensities in the LA regions above and below the Galactic plane. Additionally, a proper-motion analysis is made of a radial-velocity-selected sample of red giants and supergiants in the LMC, previously shown to be a kinematically and chemically distinct subgroup, most likely captured from the SMC. SPM4 proper motions of these stars also indicate they are distinct from the LMC population. The observational results presented here, combined with the known orbits of the Clouds and other aspects of the LMC morphology, suggest an off-center, moderate to highly inclined collision between the SMC and the LMC's disk that took place between 100 and 200 Myr ago.

  17. ACCRETION IN EVOLVED AND TRANSITIONAL DISKS IN CEP OB2: LOOKING FOR THE ORIGIN OF THE INNER HOLES

    SciTech Connect

    Sicilia-Aguilar, Aurora; Henning, Thomas; Hartmann, Lee W.

    2010-02-10

    We present accretion rates for a large number of solar-type stars in the Cep OB2 region, based on U-band observations. Our study comprises 95 members of the {approx}4 Myr old cluster Tr 37 (including 20 'transition' objects (TOs)), as well as the only classical T Tauri star (CTTS) in the {approx}12 Myr old cluster NGC 7160. The stars show different disk morphologies, with the majority of them having evolved and flattened disks. The typical accretion rates are about 1 order of magnitude lower than in regions aged 1-2 Myr, and we find no strong correlation between disk morphology and accretion rates. Although half of the TOs are not accreting, the median accretion rates of normal CTTS and accreting 'transition' disks are similar ({approx}3 x 10{sup -9} and 2 x 10{sup -9} M{sub sun} yr{sup -1}, respectively). Comparison with other regions suggests that the TOs observed at different ages do not necessarily represent the same type of objects, which is consistent with the fact that the different processes that can lead to reduced IR excess/inner disk clearing (e.g., binarity, dust coagulation/settling, photoevaporation, giant planet formation) do not operate on the same timescales. Accreting TOs in Tr 37 are probably suffering strong dust coagulation/settling. Regarding the equally large number of non-accreting TOs in the region, other processes, such as photoevaporation, the presence of stellar/substellar companions, and/or giant planet formation, may account for their 'transitional' spectral energy distributions and negligible accretion rates.

  18. Injection-attachment of Methylosinus trichosporium OB3b in a two-dimensional miniature sand-filled aquifer simulator

    SciTech Connect

    Shonnard, D.R.; Taylor, R.T.; Hanna, M.L.; Boro, C.O.; Duba, A.G. )

    1994-01-01

    For some potentially useful and emerging in situ bioremediation technologies it is important to control bacterial attachment to subsurface materials during the injection of microbial cell suspensions. In this study the attachment patterns of Methylosinus trichosporium OB3b were measured after horizontal injections into a two-dimensional miniature aquifer simulator containing a wet homogeneous sand. In preliminary sand column assays, bacterial attachment to the sand was increased nearly 2 orders of magnitude compared to attachment in the presence of distilled water by raising the concentration of a pH 7.0 sodium-potassium phosphate buffer to 10 mM. The maximal concentration of attached cells was [approximately]4 [times] 10[sup 8] cells/g dry sand with both sand minicolumns and the wet sand aquifer simulator. For the latter this occurred on streamlines directly between the horizontal injector and withdrawal ports, where injection-withdrawal velocities were the highest. The effects of a simulated groundwater cross flow during suspension injection on bacterial attachment to the aquifer simulator sands were also studied, and a peristaltic pumping method to counteract these groundwater flow effects resulted in a more localized pattern, i.e., without extensive downstream skewing of the bacterial attachment zone. Phenol red was utilized as a nonbinding, red-colored tracer compound. It proved to be very convenient for quantitatively measuring the earlier breakthroughs of cells versus an inert tracer during the aquifer simulator and subsequent capillary tube cell injection experiments and also for visualizing the anticipated boundaries of cell attachment in the aquifer simulator. The effect of injection velocity on the observed bacterial attachment patterns in these experiments appears to be accounted for by colloid filtration theory. 45 refs., 9 figs.

  19. Batch cultivation of Methylosinus trichosporium OB3B: IV. Production of hydrogen-driven soluble or particulate methane monooxygenase activity.

    PubMed

    Shah, N N; Hanna, M L; Jackson, K J; Taylor, R T

    1995-02-01

    Batch culture conditions were established for the formation of H(2)-driven whole-cell soluble or particulate methane monooxygenase (sMMO or pMMO) activity in the obligate methanotroph, Methylosinus trichosporum Ob3b, to expand its potential uses in groundwater bioremediation and the production of specific chemicals. Addition of either Ni and H(2) to a nitrate-containing minimal salts growth medium or Ni and Mo to a nitrate-lacking growth medium (induces a nitrogenase that generates intracellular H(2)) markedly enhanced both the hydrogenase and the accompanying washed-cell H(2)-driven MMO activities of shake-flask cultured cells. For sMMO containing cells, H(2) provided in vitro reducing power for the oxidation of chlorinated solvents such as chloroform and trichloroethylene. Cell cultivations under N(2)-fixing conditions in a 5-L bioreactor, however, required an initial nitrate concentration of at least 1 to 2 mM to achieve high biomass yields (5 to 7 g of dry cell wt/L) for cells producing H(2)-driven sMMO or pMMO activity. Elevation of the initial medium nitrate concentration to 20 mM shortened the culture time for pMMO producing cells by 40%, yet still generated an equivalent growth yield. High nitrate also shortened the culture time for sMMO containing cells by approximately 25%, but it lowered their biomass yield by 26%. Upon storage for 5 weeks at room temperature, washed resting-state cells retained 90% and 70% of their H(2)-driven sMMO and pMMO activity, respectively. This makes their practical use quite feasible. (c) 1995 John Wiley & Sons, Inc. PMID:18623142

  20. Accretion in Evolved and Transitional Disks in CEP OB2: Looking for the Origin of the Inner Holes

    NASA Astrophysics Data System (ADS)

    Sicilia-Aguilar, Aurora; Henning, Thomas; Hartmann, Lee W.

    2010-02-01

    We present accretion rates for a large number of solar-type stars in the Cep OB2 region, based on U-band observations. Our study comprises 95 members of the ~4 Myr old cluster Tr 37 (including 20 "transition" objects (TOs)), as well as the only classical T Tauri star (CTTS) in the ~12 Myr old cluster NGC 7160. The stars show different disk morphologies, with the majority of them having evolved and flattened disks. The typical accretion rates are about 1 order of magnitude lower than in regions aged 1-2 Myr, and we find no strong correlation between disk morphology and accretion rates. Although half of the TOs are not accreting, the median accretion rates of normal CTTS and accreting "transition" disks are similar (~3 × 10-9 and 2 × 10-9 M sun yr-1, respectively). Comparison with other regions suggests that the TOs observed at different ages do not necessarily represent the same type of objects, which is consistent with the fact that the different processes that can lead to reduced IR excess/inner disk clearing (e.g., binarity, dust coagulation/settling, photoevaporation, giant planet formation) do not operate on the same timescales. Accreting TOs in Tr 37 are probably suffering strong dust coagulation/settling. Regarding the equally large number of non-accreting TOs in the region, other processes, such as photoevaporation, the presence of stellar/substellar companions, and/or giant planet formation, may account for their "transitional" spectral energy distributions and negligible accretion rates.

  1. Measuring the rotation periods of 4-10 Myr T-Tauri stars in the Orion OB1 association

    NASA Astrophysics Data System (ADS)

    Tanveer Karim, Md; Stassun, Keivan; Briceno, Cesar; Vivas, Kathy; Raetz, Stefanie; Calvet, Nuria; Mateu, Cecilia; Downes, Juan Jose; Hernandez, Jesus; Neuhäuser, Ralph; Mugrauer, Markus; Takahashi, Hidenori; Tachihara, Kengo; Chini, Rolf; YETI

    2016-01-01

    Most existing studies of young stellar populations have focused on the youngest (< 2-3 Myr) T-Tauri stars, which are usually associated with their natal gas and hence easier to identify. In contrast, older T-Tauri stars (~ 4-10 Myr), being more difficult to find, have been less studied, even though they hold key insight to understanding evolution of lower-mass (0.1-2 M⊙) stars and of protoplanetary discs. We present a study of photometric variability of 1974 confirmed 4-10 Myr old T-Tauri stars in the Orion OB1 association using optical time-series from three different surveys: the Centro de Investigaciones de Astronomía-Quest Equatorial Survey Team (CIDA-QUEST), the Young Exoplanet Transit Initiative (YETI) and from a Kitt Peak National Observatory (KPNO) campaign. We investigated stellar rotation periods according to the type of stars (Classical or Weak-lined T-Tauri stars) and their locations, to look for population-wide trends. We detected 563 periodic variables and 1411 non-periodic variables by investigating the light curves of these stars. We find that ~ 30% of Weak-line T-Tauri stars (WTTS) and ~ 20% of Classical T-Tauri stars (CTTS) are periodic. Though we did not find any noticeable difference in rotation period between CTTS and WTTS, our study does show a change in the overall rotation periods of stars 4-10 Myr old, consistent with predictions of angular momentum evolution models, an important constraint for theoretical models for an age range for which no similar data existed.

  2. Neutron field measurements of the CRNA OB26 irradiator using a Bonner sphere spectrometer for radiation protection purposes.

    PubMed

    Mazrou, H; Allab, M

    2012-08-01

    The present work deals with the Bonner sphere spectrometer (BSS) measurements performed, to support the authors' Monte-Carlo calculations, to estimate accurately the main characteristics of the neutron field of the (241)Am-Be-based OB26 irradiator acquired for radiation protection purposes by the Nuclear Research Centre of Algiers. The measurements were performed at a reference irradiation position selected at 150 cm from the geometrical centre of the neutron source. The spectrometric system in use is based on a central spherical (3)He thermal neutron proportional counter. The response matrix of the present spectrometer has been taken to be similar to the original Physikalisch-Technische Bundesanstalt (PTB) (Braunschweig, Germany) BSS's response matrix, with a five bins per decade energy group structure, as there is no significant difference in the BSS's physical characteristics. Thereafter, the authors' BSS measurements were used together with MCNP5 results to unfold the neutron spectrum by means of MAXED and GRAVEL computer codes from the U.M.G. 3.3 package, developed at PTB. Besides, sensitivity analysis has been performed to test the consistency of the unfolding procedure. It reveals that no significant discrepancy was observed in the total neutron fluence and total ambient dose values following the perturbation of some pertinent unfolding parameters except for the case where a 10 bins energy structure was assumed for the guess spectrum. In this latter case, a 5 % difference was observed in the ambient dose equivalent compared with the reference case. Finally, a comparative study performed between different counting systems together with MCNP5 and predictive formulas results shows that they were globally satisfactory, highlighting thereby the relevance of the unfolding procedure and the reliability of the obtained results. PMID:22345213

  3. An eclipsing double-line spectroscopic binary at the stellar/substellar boundary in the Upper Scorpius OB association

    NASA Astrophysics Data System (ADS)

    Lodieu, N.; Alonso, R.; González Hernández, J. I.; Sanchis-Ojeda, R.; Narita, N.; Kawashima, Y.; Kawauchi, K.; Suárez Mascareño, A.; Deeg, H.; Prieto Arranz, J.; Rebolo, R.; Pallé, E.; Béjar, V. J. S.; Ferragamo, A.; Rubiño-Martín, J. A.

    2015-12-01

    Aims: We aim at constraining evolutionary models at low mass and young ages by identifying interesting transiting system members of the nearest OB association to the Sun, Upper Scorpius (USco), which has been targeted by the Kepler mission. Methods: We produced light curves for M-dwarf members of the USco region that has been surveyed during the second campaign of the Kepler K2 mission. We identified by eye a transiting system, USco J161630.68-251220.1 (=EPIC 203710387) with a combined spectral type of M5.25, whose photometric, astrometric, and spectroscopic properties makes it a member of USco. We conducted an extensive photometric and spectroscopic follow-up of this transiting system with a suite of telescopes and instruments to characterise the properties of each component of the system. Results: We calculated a transit duration of about 2.42 h that occurs every 2.88 days with a slight difference in transit depth and phase between the two components. We estimated a mass ratio of 0.922 ± 0.015 from the semi-amplitudes of the radial velocity curves for each component. We derived masses of 0.091 ± 0.005M⊙ and 0.084 ± 0.004M⊙, radii of 0.388 ± 0.008R⊙ and 0.380 ± 0.008R⊙, luminosities of log (L/L⊙) = -2.020-0.121+0.099 dex and -2.032-0.121+0.099 dex, and effective temperatures of 2901-172+199 K and 2908-172+199 K for the primary and secondary, respectively. Conclusions: We present a complete photometric and radial velocity characterisation of the least massive double-line eclipsing binary system in the young USco association with two components close to the stellar/substellar limit. This system falls in a gap between the least massive eclipsing binaries in the low-mass and substellar regimes at young ages and represents an important addition to constraining evolutionary models at young ages. Based on observations made with telescopes (GTC, WHT) installed in the Spanish Observatorio del Roque de los Muchachos of the Instituto de Astrofísica de

  4. X-RAY EMISSION FROM THE DOUBLE-BINARY OB-STAR SYSTEM QZ CAR (HD 93206)

    SciTech Connect

    Parkin, E. R.; Naze, Y.; Rauw, G.; Broos, P. S.; Townsley, L. K.; Pittard, J. M.; Moffat, A. F. J.; Oskinova, L. M.; Waldron, W. L.

    2011-05-01

    X-ray observations of the double-binary OB-star system QZ Car (HD 93206) obtained with the Chandra X-ray Observatory over a period of roughly 2 years are presented. The respective orbits of systems A (O9.7 I+b2 v, P{sub A} = 21 days) and B (O8 III+o9 v, P{sub B} = 6 days) are reasonably well sampled by the observations, allowing the origin of the X-ray emission to be examined in detail. The X-ray spectra can be well fitted by an attenuated three-temperature thermal plasma model, characterized by cool, moderate, and hot plasma components at kT {approx_equal} 0.2, 0.7, and 2 keV, respectively, and a circumstellar absorption of {approx_equal}0.2 x 10{sup 22} cm{sup -2}. Although the hot plasma component could be indicating the presence of wind-wind collision shocks in the system, the model fluxes calculated from spectral fits, with an average value of {approx_equal}7 x 10{sup -13} erg s{sup -1} cm{sup -2}, do not show a clear correlation with the orbits of the two constituent binaries. A semi-analytical model of QZ Car reveals that a stable momentum balance may not be established in either system A or B. Yet, despite this, system B is expected to produce an observed X-ray flux well in excess of the observations. If one considers the wind of the O8 III star to be disrupted by mass transfer, the model and observations are in far better agreement, which lends support to the previous suggestion of mass transfer in the O8 III + o9 v binary. We conclude that the X-ray emission from QZ Car can be reasonably well accounted for by a combination of contributions mainly from the single stars and the mutual wind-wind collision between systems A and B.

  5. Star formation in the filament of S254-S258 OB complex: a cluster in the process of being created

    NASA Astrophysics Data System (ADS)

    Samal, M. R.; Ojha, D. K.; Jose, J.; Zavagno, A.; Takahashi, S.; Neichel, B.; Kim, J. S.; Chauhan, N.; Pandey, A. K.; Zinchenko, I.; Tamura, M.; Ghosh, S. K.

    2015-09-01

    Infrared dark clouds are ideal laboratories for studying the initial processes of high-mass star and star-cluster formation. We investigated the star formation activity of an unexplored filamentary dark cloud (size ~5.7 pc × 1.9 pc), which itself is part of a large filament (~20 pc) located in the S254-S258 OB complex at a distance of 2.5 kpc. Using Multi-band Imaging Photometer (MIPS) Spitzer 24 μm data, we uncovered 49 sources with signal-to-noise ratios greater than 5. We identified 45 sources as candidate young stellar objects (YSOs) of Class I, flat-spectrum, and Class II natures. Additional 17 candidate YSOs (9 Class I and 8 Class II) are also identified using JHK and Wide-field Infrared Survey Explorer (WISE) photometry. We find that the protostar-to-Class II sources ratio (~2) and the protostar fraction (~70%) of the region are high. Comparison of the protostar fraction to other young clusters suggests that the star formation in the dark cloud possibly started only 1 Myr ago. Combining the near-infrared photometry of the YSO candidates with the theoretical evolutionary models, we infer that most of the candidate YSOs formed in the dark cloud are low-mass (<2 M⊙). We examine the spatial distribution of the YSOs and find that majority of them are linearly aligned along the highest column density line (N(H2)~1 × 1022 cm-2) of the dark cloud along its long axis at the mean nearest-neighbour separation of ~0.2 pc. Using the observed properties of the YSOs, physical conditions of the cloud and a simple cylindrical model, we explore the possible star formation process of this filamentary dark cloud and suggest that gravitational fragmentation within the filament should have played a dominant role in the formation of the YSOs. From the total mass of the YSOs, the gaseous mass associated with the dark cloud, and the surrounding environment, we infer that the region is presently forming stars at an efficiency of ~3% and a rate ~30 M⊙ Myr-1, and it may emerge

  6. Wide-angle OBS velocity structure and gravity modeling along the SAHKE transect, lower North Island, New Zealand

    NASA Astrophysics Data System (ADS)

    Mochizuki, K.; Henrys, S. A.; Yamada, T.; Sutherland, R.; Shinohara, M.; Iwasaki, T.; Sato, H.

    2012-12-01

    As part of the Seismic Array HiKurangi Experiment (SAHKE) project, we acquired wide-angle reflection / refraction seismic data using ocean bottom seismometers (OBSs) along a transect across the southern North Island of New Zealand, where the Hikurangi Plateau, an early Cretaceous large igneous province, which subducts westward beneath Wellington, the capital city of New Zealand. The SAHKE project was designed to investigate the physical parameters controlling locking at the plate interface beneath the southern North Island and characterize slip processes in a major segment of the Hikurangi system. We deployed 16 OBSs with 5 km spacing off the east coast and 4 OBSs with 10 km spacing off the west coast. Controlled airgun sources were shot at every 100 m along a 350 km onshore-offshore transect. Although data from OBSs at shallow depths (~100 m) contain large amplitude ambient noise, first arrivals from the airgun sources can be traced up to over 100 km offset on record sections of most OBSs. We applied first-arrival travel-time inversion in order to obtain P-wave velocity structure along the 80 km-long OBS profile off the east coast. Starting with a simple stratified velocity model including subduction structure, we iteratively revise the initial model and put more constraints on the first arrival picks. The velocity structure to ~20 km depth was resolved, and the down going slab was clearly imaged. We picked travel times of reflected waves, and projected reflection points by applying a travel-time migration method using the first arrival velocity model. Reflection interfaces including the plate interface, a prominent phase that may represent the base of the Hikurangi Plateau and an interface between the upper and lower crusts are imaged. These interfaces can also be traced westward beneath the Wellington Region and consistent with observations from onshore active source data. We also observed P-wave arrivals with very fast apparent velocities (> 8.5 km/s) on the

  7. Pinning fault zone strength using small earthquakes in the Mendocino triple junction region recorded by a dense OBS array

    NASA Astrophysics Data System (ADS)

    Chen, X.; McGuire, J. J.

    2014-12-01

    The Mendocino triple junction contains a diversity of fault types including plate boundary strike-slip and thrust faults as well as intraplate faults within the subducting oceanic mantle that are expected to operate under significantly different rheological conditions. We analyze the data from the Cascadia Initiative's Year-2 focused OBS array deployed at the triple junction region. We detect over 1000 earthquakes with magnitude ranging from 1 to 4.5 from Sep 2012 to Feb 2013. Locations refined with waveform cross-correlation arrival time measurements delineate two sub-parallel faults trending NW-SE revealing the complex geometry of the triple junction, as well as a intraplate fault possibly related to the 2010 M6.5 earthquake in the subducted Gorda plate. We are performing a joint inversion for 3D structure and hypocenter locations to further refine the image of the triple junction fault systems. Our primary focus is on examining earthquake rupture mechanics in this complex fault network. Strength envelope calculations predict that the faults within the subducting Gorda plate support differential stress levels that are 1-2 orders of magnitude larger than what is typically assumed for the thrust interface. To determine if this contrast is reflected in the data, we estimate apparent stress for the M>2 earthquakes on the three types of faults. We compute displacement spectra using 2.56s time window from the picked arrival. Spectra with signal-to-noise ratio > 3 between 4 to 10 Hz are saved for further analysis. For the saved spectra, we apply two approaches using EGF method: (1) single event-pair deconvolution, where we select best-similar event pairs using relative locations derived from waveform cross-correlation, (2) iterative stacking deconvolution, where we solve for a event term, a station term and a path term using all the event-station pairs. Once the target event source spectrum is obtained, we compute the stress drop and apparent stress using the

  8. OT1_ncalvet_1: A Deeper Look at the 3-10 Myr Old Disks in the Orion OB1 Association

    NASA Astrophysics Data System (ADS)

    Calvet, N.

    2010-07-01

    We propose to obtain deep 70/160 micron PACS Photometer observations of populations in the Orion OB1 association, spanning the critical 3 to 10 Myr range of ages when disks are supposed to dissipate and planets to form. Our ongoing large-scale survey of the Orion OB1 star-forming regions has allowed us to find the elusive low mass population in the older parts of the association, and secure the photometric and spectroscopic data to fully determine the stellar and accretion properties of these objects. We propose to obtain slow-speed scans of five 30'x30' fields, covering sections of the ~ 3 Myr old cluster sigma Ori, a ~ 5 Myr old group in the Ori OB1b subassociation, and the ~ 9 Myr old 25 Ori group, the most populous stellar group at this age within 500 pc. Our Spitzer IRAC and MIPS data for the proposed fields allow us to estimate that we will detect at least 62 disks in our PACS observations, 48% of which also have Spitzer IRS data. With the Herschel far-infrared fluxes and our mid-infrared and optical fluxes we will construct the most complete spectral energy distributions for a large sample of 3-10 Myr disks. The interpretation of these SEDs with our irradiated accretion disk codes, constrained by the stellar parameters and mass accretion rates determined independently from the UV excess, will allow the best characterization to date of disks in this age range, and provide essential constraints on theoretical models of disk evolution and planet formation.

  9. Surpoids et obésité dans la population au-dessus de 20 ans en milieu urbain bamakois (Mali)

    PubMed Central

    Oumar Bâ, Hamidou; Menta, Ichaka; Camara, Youssouf; Sangaré, Ibrahima; Sidibé, Noumou; Doumbia, Seydou; Diarra, Mamadou Bocary

    2014-01-01

    Introduction Il est question dans notre travail d’étudier le SP et l'OB et les facteurs associés dans la population âgée de 20 ans ou plus. Méthodes Notre échantillon a été obtenu à partir d'une enquête sur les pathologies cardiovasculaires dans le District de Bamako et impliquant 2199 sujets de 5-104 ans, en sélectionnant tous les sujets âgés d'au moins 20 ans (1162). Pour chaque sujet, l'IMC, rapport taille / hanche et le tour de taille ont été déterminées. Les données ont été analysées avec SPSS 12. Résultats L’âge moyen était de 36,86 années, 61,4% étaient des femmes, 49,7% dans le secteur informel et 38,0% avaient réalisé l'enseignement primaire. Facteurs de risque cardiovasculaires étaient l'inactivité physique (72,4%), le tabagisme (12,2%) et hypertension (26,7%). La prévalence de l'obésité était de 8,8 et 14,7% respectivement sur la base de l'indice de masse et le tour de taille. Conclusion Le SP et l'OB sont à prendre en compte dans les mesures de politique sanitaire que dans la pratique quotidienne des professionnels de santé, il est peut-être plus utile d'utiliser plusieurs paramètres pour être à même de bien stratifier nos patients par rapport à leur risque. PMID:25932065

  10. The history and source of particulate 137Cs and 239,240Pu deposition in sediments of the Ob River Delta, Siberia.

    PubMed

    Sayles, F L; Livingston, H D; Panteleyev, G P

    1997-08-25

    This paper presents the first results of a project designed to examine the transfer of particle-associated artificial radionuclides down the Ob River in Siberia to its delta over the past 5 decades. The main sources include fallout from atmospheric nuclear weapons tests and weapons complexes and test sites of the Former Soviet Union in or near the Ob watershed. The approach is to measure the accumulation of the radionuclides in undisturbed delta sediments-obtaining a record of their deposition over time. These records were found in sediments of shallow lakes apart from, and connected to, the main channel. Sediment cores were collected in the summer of 1994 using a shallow draft catamaran to reach these lakes from a support ship in the main channel. Measurements are presented on the depth distributions of 137Cs and Pu isotopes and their inventories in a series of dated sediment cores-including one from a location in the Taz Estuary (which does not receive Ob River sediments). Sediment dating was carried out using the excess 210Pb technique. The results obtained are compared with known information on the temporal history of releases from the various sources and characteristics of the isotopic composition of the sources. The results show that good records of radionuclide deposition indicate that the major fraction of 137Cs and Pu isotopes deposited in these delta sediments comes from atmospheric nuclear weapons test fallout-both delivered directly from the atmosphere and from downstream transport of watershed sediments. No more than 25% of the observed inventories could be derived from other sources. PMID:9241876

  11. The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold.

    PubMed

    Kuwahara, Yohta; Ohno, Ayako; Morii, Taichi; Yokoyama, Hideshi; Matsui, Ikuo; Tochio, Hidehito; Shirakawa, Masahiro; Hiroaki, Hidekazu

    2008-11-01

    Nodulation formation efficiency D (NfeD) is a member of a class of membrane-anchored ClpP-class proteases. There is a second class of NfeD homologs that lack the ClpP domain. The genes of both NfeD classes usually are part of an operon that also contains a gene for a prokaryotic homolog of stomatin. (Stomatin is a major integral-membrane protein of mammalian erythrocytes.) Such NfeD/stomatin homolog gene pairs are present in more than 290 bacterial and archaeal genomes, and their protein products may be part of the machinery used for quality control of membrane proteins. Herein, we report the structure of the isolated C-terminal domain of PH0471, a Pyrococcus horikoshii NfeD homolog, which lacks the ClpP domain. This C-terminal domain (termed NfeDC) contains a five-strand beta-barrel, which is structurally very similar to the OB-fold (oligosaccharide/oligonucleotide-binding fold) domain. However, there is little sequence similarity between it and previously characterized OB-fold domains. The NfeDC domain lacks the conserved surface residues that are necessary for the binding of an OB-fold domain to DNA/RNA, an ion. Instead, its surface is composed of residues that are uniquely conserved in NfeD homologs and that form the structurally conserved surface turns and beta-bulges. There is also a conserved tryptophan present on the surface. We propose that, in general, NfeDC domains may interact with other spatially proximal membrane proteins and thereby regulate their activities. PMID:18687870

  12. Documentation for the machine-readable version of the catalogue of individual UBV and UVBY beta observations in the region of the Orion OB1 association

    NASA Technical Reports Server (NTRS)

    Warren, W. H., Jr.

    1982-01-01

    The machine-readable files of individual UBV observations of 106 stars in the vicinity of the Orion Nebula (the Sword region) and individual uvby beta observations of 508 stars in all regions of the Orion OB 1 association are described. For the UBV data the stars are identified by their Brun numbers, with cross identifications to the chart numbers used in Warren and Hesser; the uv by beta stars are identified by the aforementioned chart numbers and HD, BD or P ( = pi); numbers in that order of preference.

  13. The VLT-FLAMES Tarantula Survey. XVI. The optical and NIR extinction laws in 30 Doradus and the photometric determination of the effective temperatures of OB stars

    NASA Astrophysics Data System (ADS)

    Maíz Apellániz, J.; Evans, C. J.; Barbá, R. H.; Gräfener, G.; Bestenlehner, J. M.; Crowther, P. A.; García, M.; Herrero, A.; Sana, H.; Simón-Díaz, S.; Taylor, W. D.; van Loon, J. Th.; Vink, J. S.; Walborn, N. R.

    2014-04-01

    Context. The commonly used extinction laws of Cardelli et al. (1989, ApJ, 345, 245) have limitations that, among other issues, hamper the determination of the effective temperatures of O and early B stars from optical and near-infrared (NIR) photometry. Aims: We aim to develop a new family of extinction laws for 30 Doradus, check their general applicability within that region and elsewhere, and apply them to test the feasibility of using optical and NIR photometry to determine the effective temperature of OB stars. Methods: We use spectroscopy and NIR photometry from the VLT-FLAMES Tarantula Survey and optical photometry from HST/WFC3 of 30 Doradus and we analyze them with the software code CHORIZOS using different assumptions, such as the family of extinction laws. Results: We derive a new family of optical and NIR extinction laws for 30 Doradus and confirm its applicability to extinguished Galactic O-type systems. We conclude that by using the new extinction laws it is possible to measure the effective temperatures of OB stars with moderate uncertainties and only a small bias, at least up to E(4405-5495) ~ 1.5 mag. Appendices are available in electronic form at http://www.aanda.org

  14. The Star-formation History and Accretion Disk Fraction Among the Low-Mass Members of the Scorpius-Centaurus OB Association

    NASA Astrophysics Data System (ADS)

    Pecaut, Mark; Mamajek, E. E.

    2012-01-01

    As part of a long-term observing program to elucidate the membership, star-formation history, and substructure of the nearest OB association (Sco-Cen; d = 100-150 pc; ages 10-20 Myr), we present results from a low-resolution optical spectroscopy survey of 363 G/K/M-type candidates of Sco-Cen selected via X-ray activity, proper-motions, and 2MASS photometry consistent with membership. Using low-resolution spectra over red ( 5700A-6800A) wavelengths obtained using the SMARTS 1.5-m telescope, we measured Li equivalent widths, determined accurate MK spectral classifications and searched for evidence of accretion among these low-mass stars ( 0.6-1.1 Msun). Using Li as a youth indicator, we evaluate membership and place each confirmed member on the H-R diagram. Combining these results with previously published results for the higher- and lower-mass stars, we discuss the substructure, age distribution, and kinematics of the Sco-Cen OB association, as well as the accretion disk fraction amongst young G/K/M-type stars in the 10-20 Myr range. This work has been supported by NSF grant AST-1008908.

  15. Psychrotolerant Endophytic Pseudomonas sp. Strains OB155 and OS261 Induced Chilling Resistance in Tomato Plants (Solanum lycopersicum Mill.) by Activation of Their Antioxidant Capacity.

    PubMed

    Subramanian, Parthiban; Mageswari, Anbazhagan; Kim, Kiyoon; Lee, Yi; Sa, Tongmin

    2015-10-01

    Studies on chilling stress damage and its mitigation through microorganisms in members of family Solanaceae is limited, despite their economic importance. We studied chilling stress alleviation in tomato plants colonized by psychrotolerant bacterial strains Pseudomonas vancouverensis OB155-gfp and P. frederiksbergensis OS261-gfp. Log phase cultures of bacterial strains were coated on surface-sterilized seeds (bacterization) before sowing and nonbacterized (control) seeds were coated with sterile bacterial growth medium. All plants were grown at temperatures of 30 and 25°C and at the end of 4 weeks, chilling treatment (12 and 10°C) was imposed for 1 week on half of the bacterized and control plants. Under normal conditions (30 and 25°C), no significant difference was observed in antioxidant activity, proline accumulation, and expression of cold acclimation genes in tomato leaf tissues of both control and bacterized plants. However, plants exposed to temperatures of 12 and 10°C were found to decrease in robustness and nutrient uptake, accompanied by increased membrane damage. Chilling resistance in bacterized plants was evident from reduced membrane damage and reactive oxygen species levels, improved antioxidant activity in leaf tissues, and high expression of cold acclimation genes LeCBF1 and LeCBF3 compared with control plants. Confocal microscopy confirmed effective colonization and intercellular localization of cold-adapted bacterial strains OB155-gfp and OS261-gfp. PMID:26075827

  16. MULTI-EPOCH VERY LONG BASELINE ARRAY OBSERVATIONS OF THE COMPACT WIND-COLLISION REGION IN THE QUADRUPLE SYSTEM Cyg OB2 no. 5

    SciTech Connect

    Dzib, Sergio A.; Rodriguez, Luis F.; Loinard, Laurent; Ortiz-Leon, Gisela N.; Araudo, Anabella T.; Mioduszewski, Amy J.

    2013-02-15

    We present multi-epoch Very Long Base Array observations of the compact wind-collision region in the Cyg OB2 no. 5 system. These observations confirm the arc-shaped morphology of the emission reported earlier. The total flux as a function of time is roughly constant when the source is 'on', but falls below the detection limit as the wind-collision region approaches periastron in its orbit around the contact binary at the center of the system. In addition, at one of the 'on' epochs, the flux drops to about a fifth of its average value. We suggest that this apparent variation could result from the inhomogeneity of the wind that hides part of the flux rather than from an intrinsic variation. We measured a trigonometrical parallax, for the most compact radio emission of 0.61 {+-} 0.22 mas, corresponding to a distance of 1.65 {sup +0.96} {sub -0.44} kpc, in agreement with recent trigonometrical parallaxes measured for objects in the Cygnus X complex. Using constraints on the total mass of the system and orbital parameters previously reported in the literature, we obtain two independent indirect measurements of the distance to the Cyg OB2 no. 5 system, both consistent with 1.3-1.4 kpc. Finally, we suggest that the companion star responsible for the wind interaction, yet undetected, is of spectral type between B0.5 and O8.

  17. The OBS Survey of Seismogenic Processes: Using 2010 Chile M=8.8 Mega Earthquake as example

    NASA Astrophysics Data System (ADS)

    Lee, C.; Wei, H.; Wang, S.

    2012-12-01

    In the early morning of February 27, 2010, a mega-earthquake now known as the "Maule Earthquake" (M=8.8) took place in central Chile. In May 1960, Chile was hit by the largest earthquake ever recorded with a magnitude of 9.5. In general, the west coast of Chile is a convergent boundary between the Nazca and South American Plates, with the Nazca Plate subducting beneath the South American Plate in a NE direction. With a convergence rate of 6-7 cm per year, stress accumulates in the lower part of the oceanic plate to a certain extent resulting in huge destructive earthquakes. In 2010, our team deployed two OBS arrays (the A and B arrays), with a total of 33 deployments to record the aftershocks along the rupture area. We collected data for a total of 46 days (July 15 to August 7 for the A array and August 14 to September 6 for the B array). The aim of our study was to analyze the distribution and characteristics of the aftershocks to get a better understanding of the tectonic activity after the main event, and conjecture on the seismogenic processes that occurred during the rupture. Using the Antelope software on the data we picked the P- and S-wave arrivals and located the events. To obtain more accurate earthquake epicenter locations we also applied the HypoDD software. We recognized a total of 3,807 events in 46 days of monitoring (in average of 80 some earthquakes per day) with many of them distributed along both sides of the trench. Immediately behind the trench axis, along the frontal accretionary prism, there is an aseismic zone, possibly due to the high content of water in the sedimentary strata. On the other hand, the paleo-accretionary prism on the landward side of the trench accumulated most of the earthquakes. These events focus at depths of 50-100 km in the subduction zone. This is called the seismogenic zone. The comparison of events before the main shock and the HypoDD results of this study show that most of the events cluster along the edge of the

  18. DUSTY OB STARS IN THE SMALL MAGELLANIC CLOUD. II. EXTRAGALACTIC DISKS OR EXAMPLES OF THE PLEIADES PHENOMENON?

    SciTech Connect

    Adams, Joshua J.; Simon, Joshua D.; Bolatto, Alberto D.; Sloan, G. C.; Sandstrom, Karin M.; Schmiedeke, Anika; Van Loon, Jacco Th.; Oliveira, Joana M.; Keller, Luke D.

    2013-07-10

    We use mid-infrared Spitzer spectroscopy and far-infrared Herschel photometry for a sample of 20 main sequence O9-B2 stars in the Small Magellanic Cloud (SMC) with strong 24 {mu}m excesses to investigate the origin of the mid-IR emission. Either debris disks around the stars or illuminated patches of dense interstellar medium (ISM) can cause such mid-IR emission. In a companion paper, Paper I, we use optical spectroscopy to show that it is unlikely for any of these sources to be classical Be stars or Herbig Ae/Be stars. We focus our analysis on debris disks and cirrus hot spots. The local, prototype objects for these models are the debris disk around Vega and the heated dust cloud surrounding the stars in the Pleiades, also known as a cirrus hot spot. These two cases predict different dust masses, radii, origins, and structures, but the cleanest classification tools are lost by the poor physical resolution at the distance of the SMC. We also consider transition disks, which would have observable properties similar to debris disks. We begin classification by measuring angular extent in the highest resolution mid-IR images available. We find 3 out of 20 stars to be significantly extended, establishing them as cirrus hot spots. We then fit the IR spectral energy distributions to determine dust temperatures and masses. Analysis yields minimum grain sizes, thermal equilibrium distances, and the resultant dust mass estimates. We find the dust masses in the SMC stars to be larger than for any known debris disks. The difference in inferred properties is driven by the SMC stars being hotter and more luminous than known debris disk hosts and not in any directly observed dust properties, so this evidence against the debris disk hypothesis is circumstantial. Finally, we created a local comparison sample of bright mid-IR OB stars in the Milky Way (MW) by cross-matching the Wide-field Infrared Survey Explorer (WISE) and Hipparcos catalogs. We find that of the thousands of nearby

  19. Analysis of High-Resolution OBS/H and MCS Data Across the Termination of a BSR in Lima Basin, Peru Margin - Velocity Structure and Paleo-BSRs

    NASA Astrophysics Data System (ADS)

    Garcia, P. G.; Pecher, I. A.; Kukowski, N.; Huebscher, C.; Clift, P. D.; Ruppel, C. D.; Bialas, J.

    2007-12-01

    Bottom simulating reflections (BSRs) in Lima Basin are confined to areas where the sub-surface structure is predicted to focus fluid flow. Most BSRs seem to be caused by free gas at the base of gas hydrate stability (BGHS). An increase of fluid flow and hence, methane flux, across the BGHS is thought to "trigger" formation of BSRs once methane concentration exceeds its solubility in pore water. Enhanced methane flux should also lead to elevated gas hydrate concentration above the BGHS. A BSR in the north of the basin coincides with outcropping layers, which often enhance fluid flow. During the GEOPECO campaign of the R/V Sonne in 2000, we deployed nine ocean bottom seismometers/hydrophones (OBS/Hs) at 1.85-km spacing across the termination of this BSR for a high-resolution OBS/H and short-streamer multichannel seismic transect. In order to investigate whether high gas hydrate concentrations are present in the hydrate zone, we compared velocities above the BSR to those in the same hydrate-free layer further landward (sequence L5 in an earlier classification). The OBS/H records were repositioned to sealevel in the τ-p domain followed by semblance analysis for determination of P-wave velocity (Vp). Vp in sequence L5 above the BSR appears lower than that at the hydrate-free site. This finding indicates that highly concentrated gas hydrates, if present at all, are confined to thin layers that do not significantly affect Vp of the entire interval. We have also identified at least two paleo-BSRs above the present BSR level. These paleo-BSRs most likely mark previous levels of the BGHS. Changes of the pressure/temperature regime must have happened sufficiently rapidly in order to "freeze" BSRs. We propose that the most likely mechanism for such rapid changes is sudden exhumation after mass wasting. The presence of at least two paleo-BSRs suggests that mass wasting has occurred repeatedly.

  20. ModObs: Atmospheric modelling for wind energy, climate and environment applications : exploring added value from new observation technique

    NASA Astrophysics Data System (ADS)

    Sempreviva, A. M.

    2009-04-01

    The EC FP6 Marie Curie Training Network "ModObs" http://www.modobs.windeng.net addresses the improvement of atmospheric boundary layer (ABL) models to investigate the interplay of processes at different temporal and spatial scales, and to explore the added value from new observation techniques. The overall goal is to bring young scientists to work together with experienced researchers in developing a better interaction amongst scientific communities of modelers and experimentalists, using a comprehensive approach to "Climate Change", "Clean Energy assessment" and "Environmental Policies", issues. This poster describes the work in progress of ten students, funded by the network, under the supervision of a team of scientists within atmospheric physics, engineering and satellite remote sensing and end-users such as companies in the private sector, all with the appropriate expertise to integrate the most advanced research methods and techniques in the following topics. MODELING: GLOBAL-TO-MESO SCALE: Analytical and process oriented numerical models will be used to study the interaction between the atmosphere and the ocean on a regional scale. Initial results indicate an interaction between the intensity of polar lows and the subsurface warm core often present in the Nordic Seas (11). The presence of waves, mainly swell, influence the MABL fluxes and turbulence structure. The regional and global wave effect on the atmosphere will be also studied and quantified (7) MESO-SCALE: Applicability of the planetary boundary layer (PBL) parametrizations in the meso-scale WRF model to marine atmospheric boundary layer (MABL) over the North Sea is investigated. The most suitable existing PBL parametrization will be additionally improved and used for downscaling North Sea past and future climates (2). Application of the meso-scale model (MM5 and WRF) for the wind energy in off-shore and coastal area. Set-up of the meso-scale model, post-processing and verification of the data from

  1. A potential model for single crystals of the Li_2O-B_2O_3 system based on non-equivalence of boron atoms

    NASA Astrophysics Data System (ADS)

    Maslyuk, V. V.; Bredow, T.; Pfnür, H.

    2004-10-01

    Ab initio calculations allow to distinguish boron atoms in BO3 and BO4 complexes in lithium borates. On this basis an effective potential model for single crystals of Li2O-B2O3 is suggested. Empirical parameters of the interaction potentials are optimized in order to reproduce the experimental data of lithium tetraborate. The optimized parameters are applied to calculations of the structures of the anhydrous borate single crystals Li3BO3, LiBO2, Li2B4O7, Li3B7O12 and LiB3O5. The range of applicability of the potential model is increased by introducing a dependence of the effective oxygen charges on the Li content. In this way good agreement with experimental data is obtained for calculated structural and elastic properties.

  2. Constraints on the Aleutian Subduction Zone from the Shumagin Gap to Kodiak Asperity from New MCS and OBS Data of the ALEUT Project

    NASA Astrophysics Data System (ADS)

    Shillington, D. J.; Nedimović, M. R.; Webb, S. C.; Bécel, A.; Delescluse, M.; Li, J.; Kuehn, H.; Biescas, B.; Wessbecher, A.; Farkas, A.; Eddy, C.; Hostetler, K.; Perls, H.; Zietman, J.; Keranen, K. M.; Louden, K. E.

    2011-12-01

    In July-August 2011, the Alaska Langseth Experiment to Understand the megaThrust (ALEUT) program acquired deep penetration multichannel seismic (MCS) reflection and ocean bottom seismometer (OBS) data along a part of the Aleutian subduction zone that exhibits the full spectrum of coupling, from locked to freely slipping. The goal of this program is to characterize variations in the geometry and properties of the megathrust, over-riding and downgoing plates and other structures and relate them to downdip and along-strike changes in slip behavior and seismogenesis. Our study encompassed 1) the freely slipping Shumagin Gap; 2) the locked Semidi segment, which last ruptured in 1938, and 3) the locked western Kodiak asperity, the western extent of the 1964 M9.2 rupture. We acquired 3700 km of MCS data with the R/V Langseth along a series of strike and dip profiles that span the entire locked zone on the megathrust (as indicated by GPS data and estimated rupture zones of past earthquakes), its updip and downdip transitions to stable sliding, bending of the downgoing plate, and preexisting structures in the oceanic crust. Data were acquired with a 6600 cu. in. air gun array and two 8-km-long streamers. The source and one of the streamers were towed at a depth of 12 m to maximize low frequencies (and deep imaging) while the second streamer was towed at 9 m for better imaging of the sediments and upper crust. Refraction data were acquired using the same source and short period OBS spaced at ~15 km along two ~400-km profiles coincident with MCS data across the Shumagin Gap and Semidi segment. Eight seismometers were also deployed onshore in the summer of 2011 that recorded the entire offshore experiment plus local and regional seismicity. Here we present initial results from MCS and OBS data regarding the megathrust and the hydration of the downgoing plate. Initial images reveal deep reflections from the megathrust from both the locked region and the estimated downdip

  3. Gels and gel-derived glasses in the Na2O-B2O3-SiO2 system. [containerless melting in space

    NASA Technical Reports Server (NTRS)

    Mukherjee, S. P.

    1982-01-01

    The containerless melting of high-purity multicomponent homogeneous gels and gel-monoliths offers a unique approach to making ultrapure multicomponent optical glasses in the reduced gravity environment of space. Procedures for preparing and characterizing gels and gel-derived glasses in the Na2O-B2O3-SiO2 system are described. Preparation is based on the polymerization reactions of alkoxysilane with trimethyl borate or boric acid and a suitable sodium compound. The chemistry of the gelling process is discussed in terms of process parameters and the gel compositions. The physicochemical nature of gels prepared by three different procedures were found to be significantly different. IR absorption spectra indicate finite differences in the molecular structures of the different gels. The melting of the gel powders and the transformation of porous gel-monoliths to transparent 'glass' without melting are described.

  4. Gels and gel-derived glasses in the system Na2O-B2O3-SiO2

    NASA Technical Reports Server (NTRS)

    Mukherjee, S. P.

    1983-01-01

    The containerless melting of high-purity multicomponent homogeneous gels and gel monoliths offers a unique approach to making ultrapure multicomponent optical glasses in the reduced gravity environment of space. Procedures for preparing and characterizing gels and gel-derived glasses in the system Na2O-B2O3-SiO2 are described. Preparation is based on the polymerization reactions of alkoxysilane with trimethyl borate or boric acid and a suitable sodium compound. The chemistry of the gelling process is discussed in terms of process parameters and the gel compositions. The physicochemical nature of gels prepared by three different procedures was found to be significantly different. Infrared absorption spectra indicate finite differences in the molecular structures of the different gels. The melting of the gel powders and the transformation of porous gel monoliths to transparent 'glass' without melting are described.

  5. Studies of hot B subdwarfs. Part 2: Energy distributions of three bright sdB/sdOB stars in the 950-5500 angstrom range

    NASA Technical Reports Server (NTRS)

    Wesemael, F.; Holberg, J. B.; Veilleux, S.; Lamontagne, R.; Fontaine, G.

    1985-01-01

    Voyager ultraviolet spectrometer observations of the subdwarf B or OB stars HD 205805, UV 1758+36 and Feige 66 are presented. All three objects display the H I Layman series in absorption. These observations are combined with low dispersion IUE spectrophotometry and with Stroemgren photometry to construct virtually complete energy distributions, which extend over the range 950-5500 angstroms. Effective temperatures based on model atmosphere calculations for high gravity, hydrogen rich stars are determined. Our analyses yield T Sub e 28,200 + or - 1300 K for HD 205805, T sub e 31, 800 + or - 1100 K for UV 1758+36, and T sub e 35,700 + or - 1500 K for Feige 66. The importance of far ultraviolet observations below L sub alpha in reducing the uncertainties associated with the interstellar extinction and the degradation of the IUE sensitivity is emphasized.

  6. Studies of hot B subdwarfs. II - Energy distributions of three bright sdB/sdOB stars in the 950-5500 A range

    NASA Technical Reports Server (NTRS)

    Wesemael, F.; Veilleux, S.; Lamontagne, R.; Fontaine, G.; Holberg, J. B.

    1985-01-01

    Voyager ultraviolet spectrometer observations of the subdwarf B or OB stars HD 205805, UV 1758+36 and Feige 66 are presented. All three objects display the H I Layman series in absorption. These observations are combined with low dispersion IUE spectrophotometry and with Stromgren photometry to construct virtually complete energy distributions, which extend over the range 950-5500 angstroms. Effective temperatures based on model atmosphere calculations for high gravity, hygrogen rich stars are determined. Our analyses yield T sub e 28,200 + or - 1300 K for HD 205805, T sub e 31, 800 + or - 1100 K for UV 1758+36, and T sub e 35,700 + or 1500 K for Feige 66. The importance of far ultraviolet observations below L sub alpha in reducing the uncertainties associated with the interstellar extinction and the degradation of the IUE sensitivity is emphasized.

  7. LiNbO3 thin-film optical waveguide grown by liquid phase epitaxy using Li2O-B2O3 flux

    NASA Astrophysics Data System (ADS)

    Yamada, Atsuo; Tamada, Hitoshi; Saitoh, Masaki

    1992-12-01

    Nearly stoichiometric LiNbO3 thin-film waveguides with high crystallinity and good surface morphology were grown on a 5 mol % MgO-doped Z-plate LiNbO3 substrate by liquid phase epitaxy using Li2O-B2O3 flux. The profile of the film-substrate interface was estimated to be nearly a step and a domain inversion with a distinct boundary at the -Z surface of the substrate was observed. Waveguiding was realized for an ordinary wave, but not for an extraordinary wave. There existed no significant optical absorption to the fundamental absorption edge, resulting in a negligibly small propagation loss of less than 1 dB/cm at a wavelength of 458 nm.

  8. Cytochrome c-554 from Methylosinus trichosporium OB3b; a protein that belongs to the cytochrome c2 family and exhibits a HALS-Type EPR signal.

    PubMed

    Harbitz, Espen; Andersson, K Kristoffer

    2011-01-01

    A small soluble cytochrome c-554 purified from Methylosinus trichosporium OB3b has been purified and analyzed by amino acid sequencing, mass spectrometry, visible, CD and EPR spectroscopies. It is found to be a mono heme protein with a characteristic cytochrome c fold, thus fitting into the class of cytochrome c(2), which is the bacterial homologue of mitochondrial cytochrome c. The heme iron has a Histidine/Methionine axial ligation and exhibits a highly anisotropic/axial low spin (HALS) EPR signal, with a g(max) at 3.40, and ligand field parameters V/ξ = 0.99, Δ/ξ = 4.57. This gives the rhombicity V/Δ = 0.22. The structural basis for this HALS EPR signal in Histidine/Methionine ligated hemes is not resolved. The ligand field parameters observed for cytochrome c-554 fits the observed pattern for other cytochromes with similar ligation and EPR behaviour. PMID:21789203

  9. Correlation between valence state of tin and elastic modulus of Sn-doped Li2O-B2O3-SiO2 glasses

    NASA Astrophysics Data System (ADS)

    Masai, Hirokazu; Matsumoto, Syuji; Ueda, Yuki; Koreeda, Akitoshi

    2016-05-01

    The correlation between the longitudinal elastic modulus c11 and tin valence state in Li2O-B2O3-SiO2 ternary glasses is investigated. Substitution of B2O3 for SiO2 increases the glass-transition temperature and c11 but decreases the melting temperature. 119Sn Mössbauer spectra show that the valence state of tin increased with increasing molten temperature, Tmolten, in air, and that it also affects the c11 value even though the 1.0 mol. % addition. The c11 values, whose error bars are governed by those of density, suggest that the addition of SnO2 increases c11 whereas SnO decreases. The relationship between Tmolten and the amount of Sn2+ suggests that the main factor affecting the tin valence state is oxygen, whose reactivity may correlate with the glass melt viscosity.

  10. Zodiacal Exoplanets in Time (ZEIT). III. A Short-period Planet Orbiting a Pre-main-sequence Star in the Upper Scorpius OB Association

    NASA Astrophysics Data System (ADS)

    Mann, Andrew W.; Newton, Elisabeth R.; Rizzuto, Aaron C.; Irwin, Jonathan; Feiden, Gregory A.; Gaidos, Eric; Mace, Gregory N.; Kraus, Adam L.; James, David J.; Ansdell, Megan; Charbonneau, David; Covey, Kevin R.; Ireland, Michael J.; Jaffe, Daniel T.; Johnson, Marshall C.; Kidder, Benjamin; Vanderburg, Andrew

    2016-09-01

    We confirm and characterize a close-in ({P}{{orb}} = 5.425 days), super-Neptune sized ({5.04}-0.37+0.34 {R}\\oplus ) planet transiting K2-33 (2MASS J16101473-1919095), a late-type (M3) pre-main-sequence (11 Myr old) star in the Upper Scorpius subgroup of the Scorpius–Centaurus OB association. The host star has the kinematics of a member of the Upper Scorpius OB association, and its spectrum contains lithium absorption, an unambiguous sign of youth (\\lt 20 Myr) in late-type dwarfs. We combine photometry from K2 and the ground-based MEarth project to refine the planet’s properties and constrain the host star’s density. We determine K2-33’s bolometric flux and effective temperature from moderate-resolution spectra. By utilizing isochrones that include the effects of magnetic fields, we derive a precise radius (6%–7%) and mass (16%) for the host star, and a stellar age consistent with the established value for Upper Scorpius. Follow-up high-resolution imaging and Doppler spectroscopy confirm that the transiting object is not a stellar companion or a background eclipsing binary blended with the target. The shape of the transit, the constancy of the transit depth and periodicity over 1.5 yr, and the independence with wavelength rule out stellar variability or a dust cloud or debris disk partially occulting the star as the source of the signal; we conclude that it must instead be planetary in origin. The existence of K2-33b suggests that close-in planets can form in situ or migrate within ∼10 Myr, e.g., via interactions with a disk, and that long-timescale dynamical migration such as by Lidov–Kozai or planet–planet scattering is not responsible for all short-period planets.

  11. A Deep X-ray look at a very massive star: HETGS spectroscopy of the blue hypergiant Cyg OB2-12 (HIP 101364)

    NASA Astrophysics Data System (ADS)

    Huenemoerder, David; Oskinova, Lidia; Ignace, Richard; Hamann, Wolf-Rainer; Schulz, Norbert S.; neilson, hilding; Shenar, Tomer

    2016-04-01

    We have obtained a Chandra/HETGS spectrum of one of the most massive and luminous stars in the Galaxy: the blue hypergiant Cyg OB2-12 (HIP 101364, spectral type B3 Ia+). This is the first measurement at high resolution of X-ray spectral lines in a blue hypergiant and allows comparison of X-ray properties between massive stars at different but related evolutionary stages: O-type supergiants, luminous blue variables, Wolf-Rayet stars, and blue hypergiants stars. The new data provide a look at how the most massive stars shed mass during their pre-supernova evolution. We find that In Cyg OB2-12 the resolved Si and Mg lines are broadened by about 1000 km/s (FWHM). The lines, however, do not show appreciable centroid shifts (<100 km/s), which would be much larger for canonical moderately thick winds (~500 km/s). The He-like Mg XI lines show evidence of photo-excitation, implying a wind origin close to the UV-bright photosphere. The spectrum also indicates relatively high temperature plasma, up to 22 MK (1.9 keV), showing significant continuum and emission lines below 5A (above 2.5 keV). Hence, at first glance, the spectrum resembles neither an O-star thick wind, nor a magnetically confined (narrow-line) plasma. We will present more detailed wind models using both X-ray and UV spectra to constrain fundamental physical parameters of this star.

  12. Mixed Pollutant Degradation by Methylosinus trichosporium OB3b Expressing either Soluble or Particulate Methane Monooxygenase: Can the Tortoise Beat the Hare?▿

    PubMed Central

    Lee, Sung-Woo; Keeney, David R.; Lim, Dong-Hee; Dispirito, Alan A.; Semrau, Jeremy D.

    2006-01-01

    Methanotrophs have been widely investigated for in situ bioremediation due to their ubiquity and their ability to degrade halogenated hydrocarbons through the activity of methane monooxygenase (MMO). It has been speculated that cells expressing the soluble form of MMO (sMMO) are more efficient in cleaning up sites polluted with halogenated hydrocarbons due to its broader substrate range and relatively fast degradation rates compared cells expressing the other form of MMO, the particulate MMO (pMMO). To examine this issue, the biodegradation of mixtures of chlorinated solvents, i.e., trichloroethylene (TCE), trans-dichloroethylene (t-DCE), and vinyl chloride (VC), by Methylosinus trichosporium OB3b in the presence of methane using either form of MMO was investigated over longer time frames than those commonly used, i.e., days instead of hours. Growth of M. trichosporium OB3b along with pollutant degradation were monitored and analyzed using a simple comparative model developed from the Ω model created for analysis of the competitive binding of oxygen and carbon dioxide by ribulose bisphosphate carboxylase. From these findings, it appears that at concentrations of VC, t-DCE, and TCE greater than 10 μM each, methanotrophs expressing pMMO have a competitive advantage over cells expressing sMMO due to higher growth rates. Despite such an apparent growth advantage, pMMO-expressing cells degraded less of these substrates at these concentrations than sMMO-expressing cells during active growth. If the concentrations were increased to 100 μM, however, not only did pMMO-expressing cells grow faster, they degraded more of these pollutants and did so in a shorter amount of time. These findings suggest that the relative rates of growth substrate and pollutant degradation are important factors in determining which form of MMO should be considered for pollutant degradation. PMID:17012599

  13. Zodiacal Exoplanets in Time (ZEIT). III. A Short-period Planet Orbiting a Pre-main-sequence Star in the Upper Scorpius OB Association

    NASA Astrophysics Data System (ADS)

    Mann, Andrew W.; Newton, Elisabeth R.; Rizzuto, Aaron C.; Irwin, Jonathan; Feiden, Gregory A.; Gaidos, Eric; Mace, Gregory N.; Kraus, Adam L.; James, David J.; Ansdell, Megan; Charbonneau, David; Covey, Kevin R.; Ireland, Michael J.; Jaffe, Daniel T.; Johnson, Marshall C.; Kidder, Benjamin; Vanderburg, Andrew

    2016-09-01

    We confirm and characterize a close-in ({P}{{orb}} = 5.425 days), super-Neptune sized ({5.04}-0.37+0.34 {R}\\oplus ) planet transiting K2-33 (2MASS J16101473-1919095), a late-type (M3) pre-main-sequence (11 Myr old) star in the Upper Scorpius subgroup of the Scorpius–Centaurus OB association. The host star has the kinematics of a member of the Upper Scorpius OB association, and its spectrum contains lithium absorption, an unambiguous sign of youth (\\lt 20 Myr) in late-type dwarfs. We combine photometry from K2 and the ground-based MEarth project to refine the planet’s properties and constrain the host star’s density. We determine K2-33’s bolometric flux and effective temperature from moderate-resolution spectra. By utilizing isochrones that include the effects of magnetic fields, we derive a precise radius (6%–7%) and mass (16%) for the host star, and a stellar age consistent with the established value for Upper Scorpius. Follow-up high-resolution imaging and Doppler spectroscopy confirm that the transiting object is not a stellar companion or a background eclipsing binary blended with the target. The shape of the transit, the constancy of the transit depth and periodicity over 1.5 yr, and the independence with wavelength rule out stellar variability or a dust cloud or debris disk partially occulting the star as the source of the signal; we conclude that it must instead be planetary in origin. The existence of K2-33b suggests that close-in planets can form in situ or migrate within ˜10 Myr, e.g., via interactions with a disk, and that long-timescale dynamical migration such as by Lidov–Kozai or planet–planet scattering is not responsible for all short-period planets.

  14. The 2010 Haiti earthquake sequence: new insight of the tectonic pattern from aftershocks and marine geophysical data : Haiti-OBS cruise

    NASA Astrophysics Data System (ADS)

    Mercier de Lepinay, B. F.; Mazabraud, Y.; Klingelhoefer, F.; Clouard, V.; Hello, Y.; Graindorge, D.; Marcaillou, B.; Crozon, J.; Saurel, J.; Charvis, P.; Mildor, B. S.; Deschamps, A.; Bouin, M.; Perrot, J.

    2010-12-01

    The devastating 2010 Haiti earthquake ruptured only a relatively short segment (~50km) of the Enriquillo-Plantain Garden fault (EPGF) a 600km long strike-slip fault running onland and offshore from Jamaica to Dominican Republic, with apparently no major surface rupture in the epicentral area. Considering the general behavior of such strike-slip fault (i.e. North Anatolian fault, San Andreas fault), we can expect that, following the 2010 earthquake, other large earthquakes will occur in the near future on adjacent segments. To contribute to the multinational scientific effort for a better understanding of the rupture process and the stress relaxation of this earthquake, we organized the Haiti-OBS cruise of the R/V L'Atalante few weeks after the catastrophe (Feb.5 to Feb.15, 2010, from and to Pointe-a-Pitre, Guadeloupe). Our goal was 1) to deploy a temporary network of seismologic stations -21 OBS, Ocean Bottom Seismometer, and 4 onland stations- and 2) to survey the detailed sea-floor features in relation with the deformation pattern of the area (multibeam bathymetry and mud-penetrator). We show that the distribution pattern of the aftershocks as well as the compressive surface structures observed in the geology and onshore/offshore morphology of the area are consistent with a deformation model implying a major left-lateral component along the EPGF, and a strong reverse component. The January 12, 2010 mainshock has been shown as very complex. However, in the first order, the mainshock and the distribution of the aftershocks, better localized by our temporary network, can be explained by the interaction between the strike-slip EPGF system and a blind folds-and-thrusts system. Thus, the general geological setting shows a southern extension until the southern part of the Canal du Sud area of the well-known fold and thrust system of the Hispaniola main block.

  15. rpoN, mmoR and mmoG, genes involved in regulating the expression of soluble methane monooxygenase in Methylosinus trichosporium OB3b.

    PubMed

    Stafford, Graham P; Scanlan, Julie; McDonald, Ian R; Murrell, J Colin

    2003-07-01

    The methanotrophic bacterium Methylosinus trichosporium OB3b converts methane to methanol using two distinct forms of methane monooxygenase (MMO) enzyme: a cytoplasmic soluble form (sMMO) and a membrane-bound form (pMMO). The transcription of these two operons is known to proceed in a reciprocal fashion with sMMO expressed at low copper-to-biomass ratios and pMMO at high copper-to-biomass ratios. Transcription of the smmo operon is initiated from a sigma(N) promoter 5' of mmoX. In this study the genes encoding sigma(N) (rpoN) and a typical sigma(N)-dependent transcriptional activator (mmoR) were cloned and sequenced. mmoR, a regulatory gene, and mmoG, a gene encoding a GroEL homologue, lie 5' of the structural genes for the sMMO enzyme. Subsequent mutation of rpoN and mmoR by marker-exchange mutagenesis resulted in strains Gm1 and JS1, which were unable to express functional sMMO or initiate transcription of mmoX. An rpoN mutant was also unable to fix nitrogen or use nitrate as sole nitrogen source, indicating that sigma(N) plays a role in both nitrogen and carbon metabolism in Ms. trichosporium OB3b. The data also indicate that mmoG is transcribed in a sigma(N)- and MmoR-independent manner. Marker-exchange mutagenesis of mmoG revealed that MmoG is necessary for smmo gene transcription and activity and may be an MmoR-specific chaperone required for functional assembly of transcriptionally competent MmoR in vivo. The data presented allow the proposal of a more complete model for copper-mediated regulation of smmo gene expression. PMID:12855729

  16. DUST PROPERTIES AND DISK STRUCTURE OF EVOLVED PROTOPLANETARY DISKS IN Cep OB2: GRAIN GROWTH, SETTLING, GAS AND DUST MASS, AND INSIDE-OUT EVOLUTION

    SciTech Connect

    Sicilia-Aguilar, Aurora; Henning, Thomas; Dullemond, Cornelis P.; Bouwman, Jeroen; Sturm, Bernhard; Patel, Nimesh; Juhasz, Attila E-mail: aurora.sicilia@uam.es

    2011-11-20

    We present Spitzer/Infrared Spectrograph spectra of 31 T Tauri stars (TTS) and IRAM/1.3 mm observations for 34 low- and intermediate-mass stars in the Cep OB2 region. Including our previously published data, we analyze 56 TTS and 3 intermediate-mass stars with silicate features in Tr 37 ({approx}4 Myr) and NGC 7160 ({approx}12 Myr). The silicate emission features are well reproduced with a mixture of amorphous (with olivine, forsterite, and silica stoichiometry) and crystalline grains (forsterite, enstatite). We explore grain size and disk structure using radiative transfer disk models, finding that most objects have suffered substantial evolution (grain growth, settling). About half of the disks show inside-out evolution, with either dust-cleared inner holes or a radially dependent dust distribution, typically with larger grains and more settling in the innermost disk. The typical strong silicate features nevertheless require the presence of small dust grains, and could be explained by differential settling according to grain size, anomalous dust distributions, and/or optically thin dust populations within disk gaps. M-type stars tend to have weaker silicate emission and steeper spectral energy distributions than K-type objects. The inferred low dust masses are in a strong contrast with the relatively high gas accretion rates, suggesting global grain growth and/or an anomalous gas-to-dust ratio. Transition disks in the Cep OB2 region display strongly processed grains, suggesting that they are dominated by dust evolution and settling. Finally, the presence of rare but remarkable disks with strong accretion at old ages reveals that some very massive disks may still survive to grain growth, gravitational instabilities, and planet formation.

  17. Spatial-temporal and genetic relationships between gold and antimony mineralization at gold-sulfide deposits of the Ob-Zaisan folded zone

    NASA Astrophysics Data System (ADS)

    Kalinin, Yu. A.; Naumov, E. A.; Borisenko, A. S.; Kovalev, K. R.; Antropova, A. I.

    2015-05-01

    The Ob-Zaisan folded zone is a fragment of a single structure composed of Paleozoic sedimentary and volcanogenic rocks (mainly black shale), which was formed at the margin of the Siberian continent and features a common set of magmatic complexes and mineral systems. However, there are some differences that determine the specific geological and metallogenic features of the Irtysh-Zaisan and Kolyvan-Tomsk fragments of the Ob-Zaisan folded zone. In the gold deposits of the West Kalba and Kolyvan-Tomsk auriferous belt, the main gold-sulfide mineralization is controlled by zones of shearing and dynamic metamorphism in carbonaceous carbonate-terrigenous rocks. This type of mineralization was formed in tectonic blocks in a compressional setting. Antimony mineralization is characterized by brecciated textures and the vein-like morphology of ore bodies, reflecting extensional tectonics. At some deposits (Zherek, Mirazh, Dalny), Sb mineralization is spatially separated from the main gold-sulfide ores and shows cross-cutting relations to the principal ore-controlling structures. In other gold deposits, stibnite is spatially associated with disseminated gold-sulfide ores and forms mineral assemblages with Ni, Co, Au, Pb, and Fe (Alimbet, Zhanan, Legostaevskoe, Semiluzhenskoe, and Kamenskoe deposits). This study reveals no direct correlation between Au and Sb in gold-sulfide ores of these deposits. SEM analysis indicated the absence of free gold in stibnite veins. However, atomic absorption and electron microprobe analysis indicated the presence of "invisible gold" from a few ppm to several tens of ppm in the stibnite. High gold contents in the gold-sulfide ores overprinted by antimony mineralization (Suzdalskoe, Zhanan, and Legostaevskoe deposits) can be explained by the processes of regeneration and redeposition. The results of microstructural observations, isotope geochronology, studies of mineral assemblages and fluid inclusions in the ores from gold deposits of the Ob

  18. Seismic structure of uppermost mantle and crust beneath West Philippine Basin and Kyusyu-Palau ridge by seafloor borehole seismometer, OBS and airgun experiment

    NASA Astrophysics Data System (ADS)

    Arisaka, M.; Shinohara, M.; Yamada, T.; Mochizuki, K.; Kaiho, Y.; Araki, E.; Nakahigashi, K.; Ito, M.; Shiobara, H.; Suehiro, K.; Kanazawa, T.

    2003-12-01

    The western Pacific area including the Philippine Sea plate is characterized by many island-arc and trench systems and back-arc basins, which are estimated to correspond to subduction of oceanic plates. The Philippine Sea plate is divided into two regions by the Kyushu-Palau Ridge running from north to south. There are the Shikoku basin and the Parece-Vela basin in the eastern part, and the West Philippine basin exists in the western part. It is important to study an uppermost mantle and crustal seismic structure of the Philippine Sea plate to consider the formation process and to estimate the development process of an island-arc and trench system. In addition, the borehole broadband seismic station (WP-1) was installed in the easternmost part of the West Philippine basin in 2001 and is in operation. To obtain the seismic structure is also important for analyses of the data retrieved from the WP-1. We conducted seismic surveys with ocean bottom seismometers (OBS), the WP-1 and controlled sources to obtain the seismic crustal structure around the WP-1 observatory March 2002, October 2002 and May 2003 using the R/V KAIREI, JAMSTEC. The total length of the profile parallel to the ridge (NS line) is about 100 km. The profile perpendicular to the ridge (EW line) has approximately 120 km long in total and crosses the ridge and the WP-1 borehole seismometer. As the controlled sources, we used a GI gun (3 liters) and Bolt 1500LL type (1500cu.in.) airguns. Number and kind of airguns differed in each experiment. Total 12 OBSs were deployed at an average interval of 10km(NS line) and 15km (EW line) for the experiments. A seismic velocity model for shallow structure is derived from using a tau-p inversion of individual OBS records. A deep structure beneath the profile is estimated by using a two-dimensional ray tracing method. The preliminary results for the NS line are obtained. P-wave velocity of the sedimentary layer is 2.7 km/s and the thickness is about 500 m. These

  19. Crustal Structure and Fracture Zone in the Central Sea Basin of the South China Sea from Wide Angle Seismic Experiments Using OBS

    NASA Astrophysics Data System (ADS)

    Ruan, Aiguo; Wei, Xiaodong

    2015-04-01

    We present two E-W trending wide-angle seismic profiles (OBS2013-ZN, OBS2014-ZN), which cross the boundary (Zhongnan fault) between the east sub-basin and the southwest sub-basin of the South China Sea (SCS). We processed the data and used 2D ray-tracing to determine the oceanic crust thickness, velocity structures and Moho depth variations related to faults. The simulated velocity models show that the sea basin of the SCS has a typical oceanic crust covered by a 1~2 km thick sediment layer with a velocity of 2~3.5 km/s. The crust has a thickness of 5~8 km, of which the oceanic layer 2 is 1.8~3 km thick, with velocity increasing downward from 4.3 km/s to 6.4 km/s, and the oceanic layer 3 is 3~5 km thick, with velocity increasing downward from 6.4 km/s~7.0 km/s. The sea basin Moho depth is approximately 10 km. The Moho discontinuity has an obvious upheaval zone with a low velocity of 7.6 km/s, which corresponds to the low velocity zone in oceanic layer 2. We constructed an arc-shaped fracture zone (40-50 km wide) by connecting the upheaval portions of the Moho in the two profiles based on seabed relief and sea mounts distribution and suggest that it is the trace of the break-up of the Macclesfield and Reed Banks caused by the triangular opening system of the southwest sub-basin. We suggest that the faults or fracture zones in the oceanic basin of the SCS were formed in segmentation with varied directions controlled by varied stress fields during the multi-episode evolution of the SCS. The seafloor spreading abrupt direction change around 116°E maybe is because of lithosphere heterogeneity caused by early episode seafloor spreading in the east sub-basin or caused by the arc-shaped fracture zone, which results in different local stress filed.

  20. A 80 OBS and 30 Land 3-component seismometers array encompassing the 280 km segment of the Lesser Antilles subduction megathrust seismogenic zone: view of current seismicity

    NASA Astrophysics Data System (ADS)

    Laigle, Mireille; Sapin, Martine; Ruiz, Mario; Diaz, Jordi; Kissling, Edi; Charvis, Philippe; Flueh, Ernst; Hirn, Alfred

    2010-05-01

    An extensive onshore and offshore seismic station array in the Lesser Antilles subduction zone allows to monitor microearthquake activity for a period of 4 months in a region previously outside of reach for detailed observation. Such a network has been possible thanks to a cluster of 3 seismic surveys (TRAIL - F/S Merian, SISMANTILLESII - N/O Atalante, and OBSANTILLES - N/O Antea) for deploying and recovering the instruments from several pools (Geoazur, INSU-IPGP, IFM-GEOMAR, AWI ). It has been followed by an additional deployment of the 28 GeoAzur OBSs (OBSANTILLES - N/O Antea) during 5 months in the south-western half. These operations have been carried out for the seismic investigation of the Antilles megathrust seismogenic zone in the framework of the THALES WAS RIGHT european project, and with also the financial support of the french ANR Catastrophes Telluriques et Tsunamis (SUBSISMANTI) and by the EU SALVADOR Programme of IFM-GEOMAR. Onshore, 30 3-components land stations (CSIC Barcelone, IPG Paris, INSU-RLBM and -LITHOSCOPE) have been temporarily deployed. The deep seismic structure of the whole area has been investigated during these seismic surveys by wide-angle reflection and refraction seismics recorded by these instruments as well as multi-channel reflection seismic imaging (MCS) along a dense grid of crossing profiles at the OBS positions providing excellent velocity information for the upper plate. Both the location and the interpretation of the recorded earthquake activity require constraints on the deep seismic structure, which will be discussed with respect to the 3D geometry of the interplate boundary and oceanic Moho, as well as those of the forearc basement and Moho. Preliminary locations have been obtained within a simple 1D velocity model by taking into account corrections for the variable thickness of the mud- and sediments layers beneath each OBS. The latter are estimated for both P- and S-waves to compensate for the huge structural

  1. A Chrono-Chemo-Kinematic Survey of the Nearest OB Association: A Front-Row Seat to a Star-Formation Story 20 Million Years in the Making

    NASA Astrophysics Data System (ADS)

    Mamajek, Eric E.

    2015-08-01

    The Scorpius-Centaurus region is the nearest large-scale star-forming complex to the Sun, spanning the southern skies from the Southern Cross to the Oph/Sco region. The complex consists of three classic subgroups defined by Blaauw with mean ages spanning ~11-16 Myr (Upper Sco, Upper Cen-Lup, Lower Cen-Cru), some smaller <10 Myr-old peripheral associations (e.g. Eta Cha, Epsilon Cha, TW Hya groups, etc.), and on-going star-formation in several molecular clouds (e.g. Cha, Lup, CrA, Oph regions). I will show that the properties of the 3 main Sco-Cen subgroups shows that the common picture of monolithic, expanding, singular "bursts" of star-formation among OB subgroups is overly simplistic. I will discuss recent results regarding the membership of the Sco-Cen groups, the chemical composition of its solar-type members, evidence for additional substructures, and summarize what Sco-Cen appears to be revealing regarding the formation and evolution of GMC complexes and dissolution of young clusters.

  2. Analysis of UBV-Photometric Observations of the Massive, Early-Type Binary System V729 Cyg (Cyg OB2 No. 5)

    NASA Astrophysics Data System (ADS)

    Antokhina, E. A.; Kumsiashvili, M. I.; Chargeishvili, K. B.

    2016-03-01

    We have analyzed photometric UBV light curves of the massive eclipsing binary system V729 Cyg which lies in the Cyg OB2 stellar association. The observations were made at the Abastumani Astrophysical Observatory in 1983-2003 as part of a program for studying binary systems of early types. The system V729 Cyg is a spectroscopic binary and consists of Of-stars. Effects associated with the collision of stellar winds are observed in it. Various researchers have assumed previously that the binary system is at an evolutionary stage of conversion into a WR+O system. The light curves have been analyzed by a synthesis method in terms of a Roche model using our own computer code analogous to the standard Wilson-Devinney code. This analysis shows that the binary system has a contact configuration and an orbital inclination of about 66 degrees. The absolute parameters of the components are estimated to be M 1 = 30 .4 M ⊙ , M 2 = 9 .2 M ⊙, R 1 = 24 .6 R ⊙ , and R 2 = 13 .8 R ⊙.

  3. Photometric observations of weak-line T Tauri stars . II. WTTS in Taurus-Auriga, Orion and Scorpius OB2-2

    NASA Astrophysics Data System (ADS)

    Chavarría-K, C.; Terranegra, L.; Moreno-Corral, M. A.; de Lara, E.

    2000-08-01

    We present uvby-$beta a photometry of 116 X-ray flux-selected active stars in the directions of the Orion (40), Taurus-Auriga (58) and Scorpius OB2-2 (18) star forming regions. Additionally, we give near IR JHK photometry of 20 active stars in the Taurus-Auriga direction. The program stars were selected from the R_\\odotsat All Sky Survey and EINSTEIN X-ray surveys and are spectroscopically confirmed weak-line T Tauri stars and weak-line T Tauri star candidates. The photometry confirms the young nature of the program stars and also indicates that a significant fraction of the sample could be foreground objects. The data given here probably represent the largest homogeneous uvby-beta a$ photometric sample of new WTTS and WTTS candidates. Many objects in the sample are observed photometrically for the first time. Based on observations collected at the Observatorio Astronómico Nacional in Sierra San Pedro Mártir, Baja California, México. Tables 1-4 are also available in electronic form at the CDS via anonymous ftp to cdsarc.u-strasbg.fr (130.79.128.5) or via http://cdsweb.u-strasbg.fr/Abstract.html

  4. Dust Masses of Disks around 8 Brown Dwarfs and Very Low-mass Stars in Upper Sco OB1 and Ophiuchus

    NASA Astrophysics Data System (ADS)

    van der Plas, G.; Ménard, F.; Ward-Duong, K.; Bulger, J.; Harvey, P. M.; Pinte, C.; Patience, J.; Hales, A.; Casassus, S.

    2016-03-01

    We present the results of ALMA band 7 observations of dust and CO gas in the disks around 7 objects with spectral types ranging between M5.5 and M7.5 in Upper Scorpius OB1, and one M3 star in Ophiuchus. We detect unresolved continuum emission in all but one source, and the 12CO J = 3 - 2 line in two sources. We constrain the dust and gas content of these systems using a grid of models calculated with the radiative transfer code MCFOST, and find disk dust masses between 0.1 and 1 M⊕, suggesting that the stellar mass/disk mass correlation can be extrapolated for brown dwarfs (BDs) with masses as low as 0.05 M⊙. The one disk in Upper Sco in which we detect CO emission, 2MASS J15555600, is also the disk with the warmest inner disk, as traced by its H-[4.5] photometric color. Using our radiative transfer grid, we extend the correlation between stellar luminosity and mass-averaged disk dust temperature, originally derived for stellar mass objects, to the BD regime to < {T}{dust}> ≈ 22{({L}*/{L}⊙ )}0.16 {{K}}, applicable to spectral types of M5 and later. This is slightly shallower than the relation for earlier spectral type objects and yields warmer low-mass disks. The two prescriptions cross at 0.27 L⊙, corresponding to masses between 0.1 and 0.2 M⊙ depending on age.

  5. NEAR-INFRARED PERIODIC AND OTHER VARIABLE FIELD STARS IN THE FIELD OF THE CYGNUS OB7 STAR-FORMING REGION

    SciTech Connect

    Wolk, Scott J.; Rice, Thomas S.; Aspin, Colin A.

    2013-04-15

    We present a subset of the results of a three-season, 124 night, near-infrared monitoring campaign of the dark clouds Lynds 1003 and Lynds 1004 in the Cygnus OB7 star-forming region. In this paper, we focus on the field star population. Using three seasons of UKIRT J, H, and K-band observations spanning 1.5 years, we obtained high-quality photometry on 9200 stars down to J = 17 mag, with photometric uncertainty better than 0.04 mag. After excluding known disk-bearing stars we identify 149 variables-1.6% of the sample. Of these, about 60 are strictly periodic, with periods predominantly <2 days. We conclude this group is dominated by eclipsing binaries. A few stars have long period signals of between 20 and 60 days. About 25 stars have weak modulated signals, but it was not clear if these were periodic. Some of the stars in this group may be diskless young stellar objects with relatively large variability due to cool starspots. The remaining {approx}60 stars showed variations which appear to be purely stochastic.

  6. Chloroform Cometabolism by Butane-Grown CF8, Pseudomonas butanovora, and Mycobacterium vaccae JOB5 and Methane-Grown Methylosinus trichosporium OB3b

    PubMed Central

    Hamamura, N.; Page, C.; Long, T.; Semprini, L.; Arp, D. J.

    1997-01-01

    Chloroform (CF) degradation by a butane-grown enrichment culture, CF8, was compared to that by butane-grown Pseudomonas butanovora and Mycobacterium vaccae JOB5 and to that by a known CF degrader, Methylosinus trichosporium OB3b. All three butane-grown bacteria were able to degrade CF at rates comparable to that of M. trichosporium. CF degradation by all four bacteria required O(inf2). Butane inhibited CF degradation by the butane-grown bacteria, suggesting that butane monooxygenase is responsible for CF degradation. P. butanovora required exogenous reductant to degrade CF, while CF8 and M. vaccae utilized endogenous reductants. Prolonged incubation with CF resulted in decreased CF degradation. CF8 and P. butanovora were more sensitive to CF than either M. trichosporium or M. vaccae. CF degradation by all three butane-grown bacteria was inactivated by acetylene, which is a mechanism-based inhibitor for several monooxygenases. Butane protected all three butane-grown bacteria from inactivation by acetylene, which indicates that the same monooxygenase is responsible for both CF and butane oxidation. CF8 and P. butanovora were able to degrade other chlorinated hydrocarbons, including trichloroethylene, 1,2-cis-dichloroethylene, and vinyl chloride. In addition, CF8 degraded 1,1,2-trichloroethane. The results indicate the potential of butane-grown bacteria for chlorinated hydrocarbon transformation. PMID:16535693

  7. Batch cultivation of Methylosinus trichosporium OB3b. 5: Characterization of poly-{beta}-hydroxybutyrate production under methane-dependent growth conditions

    SciTech Connect

    Shah, N.N.; Hanna, M.L.; Taylor, R.T.

    1996-01-20

    Methanotrophs have promising applications in the bioremediation of chlorinated hydrocarbons and in the production of a biopolymer, poly-{beta}-hydroxybutyrate (PHB). Batch bioreactor culture conditions were studied for the accumulation of PHB by methane-grown Methylosinus trichosporium OB3b, and to evaluate the effect of PHB on the bacterial capacity to degrade trichloroethylene (TCE), a common groundwater contaminant. The PHB content of the washed and lyophilized cells was measured by gas chromatography (GC), after hydrochloric acid (HCl) propanolysis. A differential GC-based assay was developed for the monomer and the polymer of {beta}-hydroxybutyrate utilizing 1% and 10% HCl (v/v) reaction mixtures, respectively. During bioreactor growth in a Cu-deficient modified Higgins` medium, the cells accumulated PHB upon depletion of nitrate. A biomass yield of 3.2 g dry wt/L and a PHB accumulation of {approximately}10% (w/w) were reached after 140 to 160 h, without adversely affecting the propene or TCE epoxidation specific rate given by whole cells containing soluble methane monooxygenase (sMMO). The TCE biotransformation capacity ({approximately}0.25 mg TCE oxidized/mg dry cell wt) of resting cells containing {approximately}10% PHB was consistently {approximately}1.6-fold greater than that of cells containing only {approximately}2% PHB. Higher levels (>10%) of accumulated PHB did not enhance this biotransformation capacity further.

  8. L'obésité en consultation cardiologique à Lomé: prévalence et facteurs de risque cardio-vasculaire associés - étude chez 1200 patients

    PubMed Central

    Pessinaba, Soulemane; Yayehd, Komlavi; Pio, Machiude; Baragou, Réné; Afassinou, Yaovi; Tchérou, Tchaa; Damorou, Findibé

    2012-01-01

    Introduction Introduction: Les objectifs de ce travail étaient de déterminer la fréquence de l'obésité et celle des autres facteurs de risque cardio-vasculaire chez ces patients obèses à Lomé (Togo). Méthodes Il s'est agi d'une étude multicentrique transversale de prévalence. Elle s'est déroulée du 05 septembre 2005 au 04 mars 2006 et a porté sur les malades vus en consultation externe dans 3 services de cardiologie de la commune de Lomé. Ont été inclus dans cette étude les malades ayant un surpoids selon les normes de l'OMS. Résultats Parmi 1200 patients vus en consultations, 779 (64,92%) avaient une surcharge pondérale. L’âge moyen était de 49,53 ± 17,24 ans. L'obésité était plus fréquente chez les femmes (79,49%) que chez les hommes (20,51%). Un antécédent d'obésité familiale (61,8%) était le principal facteur favorisant. Les autres facteurs de risque cardio-vasculaire retrouvés étaient: sédentarité (82% vs50% chez les non obèses), hypertension artérielle (54,8% vs 39,2%), alcool (50,9% vs 43,9%), dyslipidémie (34,5% vs 20%), diabète (30,9% vs 10,7%) et tabac (14,1% vs 20,3%). La différence était statistiquement significative entre les deux groupes. Les principales complications cardiovasculaires observées chez les obèses étaient: l'ischémie myocardique (26,7%), l'hypertrophie ventriculaire gauche (46,4%), la dilatation cavitaire cardiaque (30,1%) et les accidents vasculaires cérébraux (7,1%). Conclusion L'obésité est un problème de santé publique au Togo. Sa prévalence est très élevée et elle est le plus souvent associée aux autres facteurs de risque cardio-vasculaire. Des mesures préventives doivent être mises en jeu pour lutter contre ce facteur de risque. Mots clés: Obésité, prévalence, facteurs de risque. PMID:23133699

  9. Prévalence et facteurs de risque du surpoids et de l'obésité dans une population d'enfants scolarisés en milieu urbain à Sfax, Tunisie

    PubMed Central

    Regaieg, Sofien; Charfi, Nadia; Trabelsi, Lobna; Kamoun, Mahdi; Feki, Habib; Yaich, Sourour; Abid, Mohamed

    2014-01-01

    Introduction L'objectif de ce travail etait d’étudier la prévalence du surpoids et de l'obésité chez un groupe d'enfants d’âge scolaire, habitant la ville de Sfax en Tunisie, et identifier les facteurs favorisant la prise pondérale Méthodes Il s'agossait d'une enquête descriptive transversale était réalisée en 2011 sur un échantillon représentatif d’élèves recrutés dans 11 écoles primaires publiques. Des informations concernant les caractéristiques sociodémographiques, les habitudes alimentaires et le comportement sédentaire pour chaque élève ont été précisées au moyen d'un questionnaire Résultats Nous avons colligé 1529 élèves, âgés entre 9 et 12 ans et se répartissant en 787 garçons (51,14%) et 747 filles (48,86%). Selon les seuils de référence de l'IOTF, la fréquence de l'obésité était de 2,4% et celle du surpoids était de 6,3%. L'obésité était significativement associée à l'obésité parentale, un niveau socioéconomique élevé, la prise de plus de deux goûters par jour et à l'activité sédentaire. Conclusion L'identification des facteurs de risque du surpoids et de l'obésité infantile permettrait de dépister les enfants à risques afin de leur proposer des mesures de prévention adaptées. Ces mesures de prévention devraient inclure non seulement des approches individuelles, mais aussi l'environnement social et physique de l'enfant. PMID:25018807

  10. Geometry of Pacific plate in Kuril-Japan trench zones estimated from earthquake distribution using LT-OBS network and seismic structures by marine surveys

    NASA Astrophysics Data System (ADS)

    Shinohara, M.; Yamada, T.; Kuwano, A.; Nakahigashi, K.; Machida, Y.; Mochizuki, K.; Kanazawa, T.; Takanami, T.; Hino, R.

    2009-12-01

    The seismicity of the Japan arc region is as high as that observed in other areas of subduction of oceanic plates. The Japan Trench and Kuril Trench are plate convergent zones where the Pacific Plate is subducting below the Japan island. In addition, the trench is crooked off Erimo cape, Hokkaido. It is considered the bend of the trench causes complex shape of the plate boundary. There is a possibility that an asperity of a large earthquake is controlled by a shape of a plate boundary. Associated with the plate convergence, many earthquakes occur beneath landward slopes of the Japan Trench and the Kuril Trench. Such earthquakes are considered to occur mainly at plate boundary between the Pacific plate and the landward plate in landward slope of the Kuril trench and the Japan trench. Therefore, to obtain precise hypocenter distribution of earthquakes occurring in the regions is essential to estimate geometry of the plate boundary. For several years, we performed dense seafloor earthquake observation using Long-Term Ocean Bottom Seismometers (LT-OBSs) in this region, including the aftershock observation of the 2003 Tokachi-oki earthquakes which is a large interplate earthquake around the Japan island arc. In the region off Nemuro, dense seafloor observation was carried out from 2005 to 2006 for one year using LT-OBSs. In the region off Aomori, we performed the same type of a seafloor earthquake observation from 2004 to 2007 for two years in total. Ninety-two LT-OBSs were used for the observations, and an interval of the LT-OBS is approximately 20 km. The LT-OBS has three-component seismometer with a natural period of 1 Hz, and reaches a recoding period of 1 year. As a result, we obtained the precise hypocenter distribution from the region off Nemuro to the region off Aomori, and the hypocenter distribution of huge number of earthquakes enables us to estimate the geometry of the plate boundary. Additionally, seismic surveys using OBSs and controlled source were

  11. Recent advances in the Lesser Antilles observatories Part 2 : WebObs - an integrated web-based system for monitoring and networks management

    NASA Astrophysics Data System (ADS)

    Beauducel, François; Bosson, Alexis; Randriamora, Frédéric; Anténor-Habazac, Christian; Lemarchand, Arnaud; Saurel, Jean-Marie; Nercessian, Alexandre; Bouin, Marie-Paule; de Chabalier, Jean-Bernard; Clouard, Valérie

    2010-05-01

    Seismological and Volcanological observatories have common needs and often common practical problems for multi disciplinary data monitoring applications. In fact, access to integrated data in real-time and estimation of measurements uncertainties are keys for an efficient interpretation, but instruments variety, heterogeneity of data sampling and acquisition systems lead to difficulties that may hinder crisis management. In Guadeloupe observatory, we have developed in the last years an operational system that attempts to answer the questions in the context of a pluri-instrumental observatory. Based on a single computer server, open source scripts (Matlab, Perl, Bash, Nagios) and a Web interface, the system proposes: an extended database for networks management, stations and sensors (maps, station file with log history, technical characteristics, meta-data, photos and associated documents); a web-form interfaces for manual data input/editing and export (like geochemical analysis, some of the deformation measurements, ...); routine data processing with dedicated automatic scripts for each technique, production of validated data outputs, static graphs on preset moving time intervals, and possible e-mail alarms; computers, acquisition processes, stations and individual sensors status automatic check with simple criteria (files update and signal quality), displayed as synthetic pages for technical control. In the special case of seismology, WebObs includes a digital stripchart multichannel continuous seismogram associated with EarthWorm acquisition chain (see companion paper Part 1), event classification database, location scripts, automatic shakemaps and regional catalog with associated hypocenter maps accessed through a user request form. This system leads to a real-time Internet access for integrated monitoring and becomes a strong support for scientists and technicians exchange, and is widely open to interdisciplinary real-time modeling. It has been set up at

  12. Marker Exchange Mutagenesis of mxaF, Encoding the Large Subunit of the Mxa Methanol Dehydrogenase, in Methylosinus trichosporium OB3b

    PubMed Central

    Farhan Ul Haque, Muhammad; Gu, Wenyu; DiSpirito, Alan A.

    2015-01-01

    Methanotrophs have remarkable redundancy in multiple steps of the central pathway of methane oxidation to carbon dioxide. For example, it has been known for over 30 years that two forms of methane monooxygenase, responsible for oxidizing methane to methanol, exist in methanotrophs, i.e., soluble methane monooxygenase (sMMO) and particulate methane monooxygenase (pMMO), and that expression of these two forms is controlled by the availability of copper. Specifically, sMMO expression occurs in the absence of copper, while pMMO expression increases with increasing copper concentrations. More recently, it was discovered that multiple forms of methanol dehydrogenase (MeDH), Mxa MeDH and Xox MeDH, also exist in methanotrophs and that the expression of these alternative forms is regulated by the availability of cerium. That is, expression of Xox MeDH increases in the presence of cerium, while Mxa MeDH expression decreases in the presence of cerium. As it had been earlier concluded that pMMO and Mxa MeDH form a supercomplex in which electrons from Mxa MeDH are back donated to pMMO to drive the initial oxidation of methane, we speculated that Mxa MeDH could be rendered inactive through marker-exchange mutagenesis but growth on methane could still be possible if cerium was added to increase the expression of Xox MeDH under sMMO-expressing conditions. Here we report that mxaF, encoding the large subunit of Mxa MeDH, could indeed be knocked out in Methylosinus trichosporium OB3b, yet growth on methane was still possible, so long as cerium was added. Interestingly, growth of this mutant occurred in both the presence and the absence of copper, suggesting that Xox MeDH can replace Mxa MeDH regardless of the form of MMO expressed. PMID:26712545

  13. Short-term treatment with oleoyl-oestrone in liposomes (Merlin-2) strongly reduces the expression of the ob gene in young rats.

    PubMed Central

    Sanchis, D; Adán, C; Ardévol, A; Del Mar Grasa, M; Cabot, C; Balada, F; Vilà, R; Estruch, J; Puerta, M; Fernández-López, J A; Remesar, X; Alemany, M

    1997-01-01

    Young female rats of 160-180 g were implanted with osmotic minipumps releasing 3.0 micromol/day per kg of oleoyl-oestrone in liposomes (Merlin-2) into the bloodstream for up to 14 days. Merlin-2 induced a loss of appetite in the first days, later recovered, and a decrease in body weight of 7%, which contrasts with the 15% increase in controls during the 2-week period. Neither plasma glucose nor urea was affected by treatment, but liver glycogen increased by 50% in 14 days. Insulin decreased slightly with Merlin-2 treatment. Plasma corticotropin (ACTH) and corticosterone showed a transient increase by day 6 of treatment. The expression of the ob gene in adipose tissue fell during the period studied to practically nil on day 14; circulating leptin levels decreased more than 70% from day 1 to day 14. Oestrone levels increased from 0.3 nM (controls) to a maintained 40-60 nM level for the rest of the experiment. Oleoyl-oestrone levels first increased 4-fold, to decrease again to the initial levels on day 10, increasing later to 100-fold on day 14. The three phases observed in food intake, weight loss and oleoyl-oestrone levels match fairly well, which supports the direct involvement of oleoyl-oestrone in body-weight control. However, the control of oleoyl-oestrone levels seems to be mediated in part by corticosterone. The practical disappearance of leptin synthesis coincides with the massive accumulation of oleoyl-oestrone in plasma. The results presented suggest the involvement of oleoyl-oestrone in the main mechanisms of control of body weight and its regulation by glucocorticoids and leptin. PMID:9291105

  14. The Virtual Observatory Service TheoSSA: Establishing a Database of Synthetic Stellar Flux Standards II. NLTE Spectral Analysis of the OB-Type Subdwarf Feige 110

    NASA Technical Reports Server (NTRS)

    Rauch, T.; Rudkowski, A.; Kampka, D.; Werner, K.; Kruk, J. W.; Moehler, S.

    2014-01-01

    Context. In the framework of the Virtual Observatory (VO), the German Astrophysical VO (GAVO) developed the registered service TheoSSA (Theoretical Stellar Spectra Access). It provides easy access to stellar spectral energy distributions (SEDs) and is intended to ingest SEDs calculated by any model-atmosphere code, generally for all effective temperatures, surface gravities, and elemental compositions. We will establish a database of SEDs of flux standards that are easily accessible via TheoSSA's web interface. Aims. The OB-type subdwarf Feige 110 is a standard star for flux calibration. State-of-the-art non-local thermodynamic equilibrium stellar-atmosphere models that consider opacities of species up to trans-iron elements will be used to provide a reliable synthetic spectrum to compare with observations. Methods. In case of Feige 110, we demonstrate that the model reproduces not only its overall continuum shape from the far-ultraviolet (FUV) to the optical wavelength range but also the numerous metal lines exhibited in its FUV spectrum. Results. We present a state-of-the-art spectral analysis of Feige 110. We determined Teff =47 250 +/- 2000 K, log g=6.00 +/- 0.20, and the abundances of He, N, P, S, Ti, V, Cr, Mn, Fe, Co, Ni, Zn, and Ge. Ti, V, Mn, Co, Zn, and Ge were identified for the first time in this star. Upper abundance limits were derived for C, O, Si, Ca, and Sc. Conclusions. The TheoSSA database of theoretical SEDs of stellar flux standards guarantees that the flux calibration of astronomical data and cross-calibration between different instruments can be based on models and SEDs calculated with state-of-the-art model atmosphere codes.