Mohamed Mohamed El-Shazly
Oleander (Nerium oleander L., Apocynaceae) is an evergreen urbanite shrub, widely used for ornamental purposes in Egypt. Although this plant is naturally protected from several herbivores by its defensive secondary metabolites, it harbors many phytophagous pests. In the present work, a sampling program was conducted for two years, extended from July 1998 to June 2000, in Giza city, Egypt, to
Bakkali, H; Ababou, M; Nassim Sabah, T; Moussaoui, A; Ennouhi, A; Fouadi, F Z; Siah, S; Ihrai, H
Nerium Oleander is a shrub that grows naturally in the Mediterranean regions. In Morocco it is found in wet places. It is famous for its risk of systemic toxicity in cases of poisoning because of the presence of two alkaloids, especially oleandrine. The literature describes cases of local use of leaves of this plant against scabies, haemorrhoids, and boils. We report two cases of chemical burns of different gravity due to Nerium Oleander. This should lead to more widely diffused information for the general population and strict regulation of its marketing. PMID:21991211
Bhuvaneshwari, L.; Arthy, E.; Anitha, C; Dhanabalan, K; Meena, M
Nerium indicum [Family:Apocynaceae] is commonly known as Arali [Tam] found throughout India, and has been used in the treatment of cancer, cardiotonic, leprosy and skin diseases. Plant parts such as root, bark and leaves are used. The present study is therefore undertaken to analyse its phyto chemical constituents in solvents like Benene, Chloroform and Alcohol and to screen its antibacterial activity. The dried leaf sample is extracted with solvents by cold maceration. The phytochemical analysis showed the presence of Alkaloids, Terpenoids, Cardiac glycosides, Saponins, Tannins & Carbohydrates in all the solvents. All the extracts were screened for antibacterial activity by Disc Diffusion Method. Out of the cultures used Staphylococccus aureus, Pseudomonas aeruginosa and Salmonella typhimurium showed better zone of inhibition which is 10mm, 9mm & 7 mm respectively. PMID:22557246
Schwartz, William Lewis
THE TOXICITY OF NERIUM OLEANDER IN THE MONKEY (CERUS DELLA): A PATHOLOGIC STUDY A Thesis by WILLIAM LEWIS SCHWARTZ Submitted to the Graduate College of Texas ASM University in partial fulfillment of the requirements for the degree of MASTER... OF SCIENCE August 1970 Major Subject: Veterinary Pathology THE TOXICITY OF NERIUM OLEANDER IN THE MONKEY (CEBUS APELLA): A PATHOLOGIC STUDY A Thesis by WILLIAM LEWIS SCHWARIZ Approved as to style and content by: (Chair an of Committee) (Head...
Stockstill, Barbara Layne
: ~A1 \\ ' (8' ~ ', 1978), A. (911 dhhlb 8, 1988)) d 1 h Ebb bd: ~Eh bh h 1 (Marty, 1968), Ficus carica (Rachmilevitz and Fahn, 1982), F. elastica (Heinrich, 1970). The formation of the central vacuole in the nonarticu- lated, branched laticifers... of tt tl 1 t d 1 1 tf ' ~J* 6 mddt (C, 1969). Oleander nuclei were often lobed and were surrounded by a nuclear envelope containing pores. Rachmilevitz and Fahn (1982) reported lobed nuclei in F. carica and Wilson and Mahlberg (1978) observed nuclear...
Hadizadeh, I; Peivastegan, B; Kolahi, M
Anti-mycotic activity of the ethanol extracts from Nettle (Urtica dioica L.), Colocynth (Citrullus colocynthis L. Schrad), Konar (Ziziphus spina-christi L.) and Oleander (Nerium oleander L.) floral parts were screened in vitro against four important plant pathogenic fungi viz.; Alternaria alternate, Fusarium oxysporum, Fusarium solani and Rizoctonia solani using agar dilution bioassay. Extracts showed antifungal activity against all the tested fungi. Among the plants, Nettle and Colocynth were the most effective against A. alternate and R. solani while Oleander possesses the best inhibition on F. oxysporum and F. solani. Konar was the most effective extract by reducing the growth of Rizoctonia solani than other fungi. These results showed that extracts could be considered suitable alternatives to chemical additives for the control of fungal diseases in plants. PMID:19579919
Loredana Culotta; Antonio Gianguzza; Santino Orecchio
Polycyclic Aromatic Hydrocarbons (PAH) were determined in the leaves of Nerium oleander L. an evergreen plant that occurs widely in both urban and rural areas, to monitor the degree of pollution in the urban area of Palermo (Italy) compared to remote areas. Twenty sites (urban roadside, urban, urban park, suburban and rural) in and around Palermo city were investigated.The purpose
Kevin J. Delaney
Insect herbivory has variable effects on plant physiology; so greater understanding is needed about how injury alters photosynthesis\\u000a on individual injured and uninjured leaves. Gas exchange and light-adapted leaf chlorophyll fluorescence measurements were\\u000a collected from uninjured and mechanical partial leaf defoliation in two experiments with Nerium oleander (Apocynaceae) leaves, and one experiment with Danaus plexippus herbivory on Asclepias curassavica (Asclepiadaceae)
Purcell, A H; Saunders, S R; Hendson, M; Grebus, M E; Henry, M J
ABSTRACT A lethal leaf scorch disease of oleander (Nerium oleander) appeared in southern California in 1993. A bacterium, Xylella fastidiosa, was detected by culturing, enzyme-linked immunoassay, and polymerase chain reaction in most symptomatic plants but not in symptomless plants or negative controls. Inoculating oleanders mechanically with X. fastidiosa cultures from diseased oleanders caused oleander leaf scorch (OLS) disease. The bacterium was reisolated from inoculated plants that became diseased. Three species of xylem sap-feeding leafhoppers transmitted the bacterium from oleander to oleander. The bacterium multiplied, moved systemically, and caused wilting in Madagascar periwinkle (Catharanthus rosea) and leaf scorch in periwinkle (Vinca major) in a greenhouse after inoculation with needle puncture. No bacterium was reisolated from grapevine (Vitis vinifera), peach (Prunus persica), olive (Olea europaea), California blackberry (Rubus ursinus), or valley oak (Quercus lobata) mechanically inoculated with OLS strains of X. fastidiosa. A 500-bp sequence of the 16S-23S ribosomal intergenic region of oleander strains showed 99.2% identity with Pierce's disease strains, 98.4% identity with oak leaf scorch strains, and 98.6% identity with phony peach, plum leaf scald, and almond leaf scorch strains. PMID:18944803
Abbasi, Muddasir Hassan; Fatima, Sana; Naz, Naila; Malik, Ihtzaz A.
To gain insight into the hepatohistological alterations in noninjured rat liver, Nerium oleander (N.O.) leaves extract was injected intramuscularly to induce an acute phase reaction (APR). Histopathological changes were studied after 3, 12, and 24?h time course of sterile muscle abscess. Tissue integrity and any infiltration of inflammatory cells in the liver were investigated by Hematoxylin and Eosin and ED1 peroxidase stainings. The administration of N.O. leaves extract (10?mL/kg) in H & E stained sections showed a general vacuolization of cytoplasm resulting loss of polarity with prominent nucleoli after 3?h of induction. At 12?h, eccentric nuclei were also observed in the sections. Marked infiltration of leucocytes with predominate macrophages was also found after 24?h as seen by ED1 positive staining. In the present study, a possible relationship between serum hepcidin and total iron level was also investigated in vivo. An early increase of hepcidin and total iron level (3?h) with a maximum at 12?h (P < 0.01; P < 0.001) was observed. These changes indicate that sterile muscle abscess may induce APR resulting in hepatic damage which is evident with the recruitment of inflammatory cells into the organ. PMID:24069586
Benson, Kathleen F; Newman, Robert A; Jensen, Gitte S
Objective The goal for this study was to evaluate the effects of an Aloe vera-based Nerium oleander extract (NAE-8®), compared to an extract of A. vera gel alone (ALOE), and to an aqueous extract of N. oleander (AQ-NOE) in bioassays pertaining to dermatologic potential with respect to antioxidant protection, anti-inflammatory effects, and cytokine profiles in vitro. Methods Cellular antioxidant protection was evaluated in three separate bioassays: The cellular antioxidant protection of erythrocytes (CAP-e) assay, protection of cellular viability and prevention of apoptosis, and protection of intracellular reduced glutathione levels, where the last two assays were performed using human primary dermal fibroblasts. Reduction of intracellular formation of reactive oxygen species (ROS) was tested using polymorphonuclear cells in the absence and presence of oxidative stress. Changes to cytokine and chemokine profiles when whole blood cells and human primary dermal fibroblasts were exposed to test products were determined using a 40-plex Luminex array as a method for exploring the potential cross-talk between circulating and skin-resident cells. Results The NAE-8® provided significantly better antioxidant protection in the CAP-e bioassay than AQ-NOE. NAE-8® and AQ-NOE both protected cellular viability and intracellular reduced glutathione, and reduced the ROS formation significantly when compared to control cells, both under inflamed and neutral culture conditions. ALOE showed minimal effect in these bioassays. In contrast to the NAE-8®, the AQ-NOE showed induction of inflammation in the whole blood cultures, as evidenced by the high induction of CD69 expression and secretion of a number of inflammatory cytokines. The treatment of dermal fibroblasts with NAE-8® resulted in selective secretion of cytokines involved in collagen and hyaluronan production as well as re-epithelialization during wound healing. Conclusion NAE-8®, a novel component of a commercial cosmetic product, showed beneficial antioxidant protection in several cellular models, without the induction of leukocyte activation and secretion of inflammatory cytokines. The biological efficacy of NAE-8® was unique from both ALOE and AQ-NOE. PMID:26005354
Mueller, Erin C; Day, Thomas A
We assessed how small patches of contrasting urban ground cover [mesiscape (turf), xeriscape (gravel), concrete, and asphalt] altered the microclimate and performance of adjacent oleander (Nerium oleander L.) plants in Phoenix, Arizona during fall/winter (September-February) and spring/summer (March-September). Ground-cover and oleander canopy surface temperatures, canopy air temperatures and pot soil temperatures tended to be lowest in the mesiscape and highest in the asphalt and concrete. Canopy air vapor pressure deficits were lowest in the mesiscape and highest in the asphalt plot. Rates of net photosynthesis of all oleander plants were highest in October and May, and declined through mid-summer (June-July), when rates tended to be highest in the cooler mesiscape, particularly when water was limiting. During fall/winter, oleanders in the mesiscape produced 20% less biomass, 13% less leaf area, and had 12% lower relative growth rates (R(G)) than those in the other ground covers. Lower nighttime temperatures in the mesiscape in December led to oleander frost damage. During spring/summer, oleanders in the mesiscape produced 11% more biomass, 16% more leaf area, and had 3% higher R(G) than those in the other cover types. The effects of urban ground cover on oleander performance were season-specific; while oleander growth was greatest in the mesiscape during spring/summer, it was lowest during fall/winter and these plants experienced frost damage. Because all oleander plants produced >10 times as much biomass during the spring/summer, on an annual basis oleanders in the mesiscape produced 5-11% more biomass than plants in the warmer ground covers. PMID:15726447
Stockstill, Barbara Layne
and adaptation to water stress in Nezium oleandez. Carnegie Inst. Wash. Yeaz Book 1979, pp. 150-157. Buvat, R. and G. Robert. 1979. Vacuole fozmation in the actively growing zoot meristem of barley (Hordeum sativum). Amez. J. Bot. 66(10): 1219-1237. Casa, D...
Schwartz, William Lewis
revealed initial slowing of sinus rhythm, prolongation of the P-R interval, atrio-ventricular dissociation, development of idioventricular rhythm and cessation of auricular activity. There was widening of the QRS complexes, left axis deviation.... The vagal, or inhibitory, effects are characterized by slowed rhythm, diastolic tendency and reduced conductivity of the impulse Independent ventricular rhythm develops due to blocking of the auricular impulse by lessened conductivity. The time output...
Variable indirect photosynthetic rate (Pn) responses occur on injured leaves after insect herbivory. It is important to understand factors that influence indirect Pn reductions after injury. The current study examines the relationship between gas exchange and chlorophyll a fluorescence parameters wi...
Cortinovis, Cristina; Caloni, Francesca
This review focuses on some of the most important poisonous plants in Europe and provides an overview of the poisoning episodes that have occurred in European countries. Poisoning of livestock and companion animals by plants is a relatively common occurrence. In Europe livestock and horses are commonly poisoned by Datura stramonium (Jimson weed), Senecio spp. (ragworts and groundsels), Quercus spp. (oak), Taxus baccata (European yew), Nerium oleander (oleander), Pteridium aquilinum (bracken fern), Robinia pseudoacacia (black locust) and Rhododendron spp. (rhododendrons and azaleas). Poisoning may occur when the fresh plant is ingested in pasture or when it contaminates hay or silage. In pets, the greatest majority of plant poisonings are the result of ingestion of house or garden plants, such as Cycas revoluta (Sago palm), Ricinus communis (castor bean), Allium spp., Euphorbia pulcherrima (poinsettia), Lilium spp., Convallaria majalis (Lily of the valley), Pyracantha spp. (firethorn), Rhododendron spp. (rhododendrons and azaleas), Melia azedarach (Chinaberry tree), Taxus baccata (European yew) and Nerium oleander (oleander). PMID:23570777
Narváez-Reinaldo, Juan Jesús; García-Fontana, Cristina; Vílchez, Juan Ignacio; González-López, Jesús
Arthrobacter koreensis 5J12A is a desiccation-tolerant organism isolated from the Nerium oleander rhizosphere. Here, we report its genome sequence, which may shed light on its role in plant growth promotion. This is believed to be the first published genome of a desiccation-tolerant plant growth promoter from the genus Arthrobacter. PMID:26067978
Neil C. Turner; E.-D. Schulze; T. Gollan
The responses of photosynthesis, transpiration and leaf conductance to changes in vapour pressure deficit were followed in well-watered plants of the herbaceous species, Helianthus annuus, Helianthus nuttallii, Pisum sativum and Vigna unguiculata, and in the woody species having either sclerophyllous leaves, Arbutus unedo, Nerium oleander and Pistacia vera, or mesomorphic leaves, Corylus avellana, Gossypium hirsutum and Prunus dulcis. When the
Vinayagam, A.; Sudha, P. N.
Four major compounds were separated and identified from the methanol extracts of Nerium indicum flowers (Arali) using HPLC and mass spectral data. Through mass data, the chemical structures were elucidated as: trans5-O-caffeoylquinic acid (1), quercetin-3-O- rutinoside (2), luteolin-5-O-rutinoside (3) and luteolin-7-O-rutinoside (4). In addition, the cis isomers of 5-O-caffeoylquinic acid in Nerium indicum flowers were confirmed by Mass, HPLC and UV. The structures of these compounds confirmed with the help of liquid chromatography mass spectrometry. PMID:25767323
Adrover, M; Forss, A L; Ramon, G; Vadell, J; Moya, G; Taberner, A Martinez
Growth and nutrient uptake of seven tree species were evaluated with the goal of selecting the species that can be used for wastewater enhancement by dendro-purification, or green tree filtering, and for restoration of riparian woodlands. Trees were grown in pots with an inert mixture of perlite and vermiculite and irrigated with either nutrient solution or treated wastewater We measured the effects of species and irrigation water on biomass and nutrient content of leaves, stems and roots. For most of the species, treated wastewater had a positive effect on final biomass and above ground: below ground ratio compared to that of nutrient solution. However, growth of Cupressus sempervirens and Populus nigra were inhibited by water sodium concentration. Nerium oleander, Tamarix africana and Vitex agnus-castus were the species with the greatest final biomass. Pistacia terebinthus had the highest nitrogen and phosphorus content in leaves, stems and roots, while N. oleander and V. agnus-castus showed the best potassium accumulation. In general, P. terebinthus, N. oleander, T. africana and V. agnus-castus were the best qualified species for purification of wastewater. PMID:18972692
Ganteaume, Anne; Jappiot, Marielle; Lampin, Corinne; Guijarro, Mercedes; Hernando, Carmen
Assessment of the flammability of ornamental vegetation (particularly hedges) planted around houses is necessary in light of the increasing urbanization of the wildland-urban interfaces (WUIs) and the high fire occurrence in such areas. The structure and flammability of seven of the species most frequently planted as hedges in Provence (southeastern France) were studied at particle level. Spatial repartition of the different types of fuel particles within plants was assessed by means of the cube method. The leaf flammability was assessed using an epiradiator as a burning device, and measurements of foliar physical characteristics and gross heat of combustion (GHC) helped to explain the results of burning experiments. Co-inertia analysis revealed that species with thin leaves were quick to ignite (Pyracantha coccinea, Phyllostachys sp.) and species with high leaf GHC burned the longest (Pittosporum tobira, Nerium oleander). Species presenting high ignitability (Photinia fraseri, Phyllostachys sp. and Pyracantha coccinea) were characterized by high foliar surface area-to-volume ratio, and species presenting lower ignitability were characterized by high GHC (Pittosporum tobira, Nerium oleander, Cupressus sempervirens). Hierarchical cluster analysis of the flammability variables (ignition frequency, time-to-ignition and flaming duration) categorized the relative flammability of the seven species (including dead Cupressus sempervirens) in five clusters of species from poorly flammable (Pittosporum tobira) to extremely flammable (dead Cupressus sempervirens).This study provides useful information for reducing fire risk in WUIs in the study area. PMID:23765042
Ne'eman, Gidi; Izhaki, Ido
The unripe fruits of certain species are red. Some of these species disperse their seeds by wind (Nerium oleander, Anabasis articulata), others by adhering to animals with their spines (Emex spinosa) or prickles (Hedysarum spinosissimum). Certainly neither type uses red coloration as advertisement to attract the seed dispersing agents. Fleshy-fruited species (Rhamnus alaternus, Rubus sanguineus and Pistacia sp.), which disperse their seeds via frugivores, change fruit color from green to red while still unripe and then to black or dark blue upon ripening. The red color does not seem to function primarily in dispersal (unless red fruits form advertisement flags when there are already black ripe fruits on the plant) because the red unripe fruits of these species are poisonous, spiny, or unpalatable. The unripe red fruits of Nerium oleander are very poisonous, those of Rhamnus alaternus and Anabasis articulata are moderately poisonous, those of Rubus sanguineus are very sour, those of Pistacia sp. contain unpalatable resin and those of Emex spinosa and Hedysarum spinosissimum are prickly. We propose that these unripe red fruits are aposematic, protecting them from herbivory before seed maturation. PMID:19847110
Erdemoglu, Nurgun; Küpeli, Esra; Ye?ilada, Erdem
Ethanolic and aqueous extracts from seven plant species used in Turkish traditional medicine were evaluated for in vivo anti-inflammatory and antinociceptive activities; Helleborus orientalis Lam. roots and herbs, Juglans regia L. leaves, Laurocerasus officinalis Roemer leaves, Nerium oleander L. dried and fresh flowers and leaves, Rhododendron ponticum L. leaves, Rubus hirtus Walds. et Kit aerial parts and Rubus sanctus Schreber aerial parts and roots. All the plant extracts, except the aqueous extract of Rubus hirtus, were shown to possess significant antinociceptive activity in varying degrees against p-benzoquinone-induced abdominal contractions in mice. However, only the ethanolic extracts of Helleborus orientalis roots, Juglans regia leaves, Laurocerasus officinalis leaves, Nerium oleander dried and fresh flowers, and Rhododendron ponticum leaves exhibited potent anti-inflammatory activity against carrageenan-induced hind paw edema model in mice without inducing any gastric damage. Results of the present study confirmed the folkloric claim that all the selected materials to possess potent antinociceptive and anti-inflammatory activity. PMID:14522443
Dai, Lingpeng; Wang, Wanxian; Dong, Xinjiao; Hu, Renyong; Nan, Xuyang
Cardiac glycosides from fresh leaves of Nerium indicum were evaluated for its molluscicidal activity against Pomacea canaliculata (golden apple snail: GAS) under laboratory conditions. The results showed that LC(50) value of cardiac glycosides against GAS was time dependent and the LC(50) value at 96 h was as low as 3.71 mg/L, which was comparable with that of metaldehyde at 72 h (3.88 mg/L). These results indicate that cardiac glycosides could be an effective molluscicide against GAS. The toxicological mechanism of cardiac glucosides on GAS was also evaluated through changes of selected biochemical parameters, including cholinesterase (ChE) and esterase (EST) activities, glycogen and protein contents in hepatopancreas tissues of GAS. Exposure to sublethal concentrations of cardiac glycosides, GAS showed lower activities of EST isozyme in the later stages of the exposure period as well as drastically decreased glycogen content, although total protein content was not affected at the end of 24 and 48 h followed by a significant depletion at the end of 72 and 96 h. The initial increase followed by a decline of ChE activity was also observed during the experiment. These results suggest that cardiac glycosides seriously impair normal physiological metabolism, resulting in fatal alterations in major biochemical constituents of hepatopancreas tissues of P. canaliculata. PMID:21843803
Pre-introductory host specificity tests were performed with Encarsia diaspidicola, a biological control candidate against the invasive white peach scale, Pseudaulacaspis pentagona. False oleander scale, P. cockerelli, coconut scale, Aspidiotus destructor, cycad scale, Aulacaspis yasumatsui, greenh...
Dai, Lingpeng; Qian, Xiaowei; Nan, Xuyang; Zhang, Yejian
Cardiac glycosides from Nerium indicum showed potent molluscicide activity against Pomacea canaliculata (GAS), but the toxicological mechanism is still far less understood. Effects of sublethal treatments of cardiac glycosides on feeding rate, digestive enzymes and ultrastructural alterations of the hepatopancreas in GAS were evaluated in this study. Exposure of GAS to sublethal concentrations of cardiac glycosides resulted in a significant reduction of feeding rate of GAS. The amylase, cellulose and protease activity were increase significantly at the end of 24 h followed by significant inhibition after 48 h of exposure while lipase activity was not affected significantly at the end of 24 h followed by a significant inhibition after 48 h of exposure during experimental period. The main ultrastructural alterations of hepatopancreas observed in snails under cardiac glycosides treatment comprised disruption of nuclear membrane, increased vesiculation and dilatation of endoplasmic reticulum, and vacuolization and swelling of mitochondrial compared to the untreated GAS. These results, for the first time, provide systematic evidences showing that cardiac glycosides seriously impairs the hepatopancreas tissues of GAS, resulting in inhibition of digestive enzymes activity and feeding rate and cause GAS death in the end. PMID:24361644
Wong, Siu Kuin; Lim, Yau Yan; Ling, Sui Kiong; Chan, Eric Wei Chiang
Background: Three compounds isolated from the methanol (MeOH) leaf extract of Vallaris glabra (Apocynaceae) were those of caffeoylquinic acids (CQAs). This prompted a quantitative analysis of their contents in leaves of V. glabra in comparison with those of five other Apocynaceae species (Alstonia angustiloba, Dyera costulata, Kopsia fruticosa, Nerium oleander, and Plumeria obtusa), including flowers of Lonicera japonica (Japanese honeysuckle), the commercial source of chlorogenic acid (CGA). Materials and Methods: Compound were isolated by column chromatography, and identified by NMR and MS analyses. CQA content of leaf extracts was determined using reversed-phase HPLC. Results: From the MeOH leaf extract of V. glabra, 3-CQA, 4-CQA, and 5-CQA or CGA were isolated. Content of 5-CQA of V. glabra was two times higher than flowers of L. japonica, while 3-CQA and 4-CQA content was 16 times higher. Conclusion: With much higher CQA content than the commercial source, leaves of V. glabra can serve as a promising alternative source. PMID:24497746
Mott, Keith A; Peak, David
This study tests two predictions from a recently proposed model for stomatal responses to humidity and temperature. The model is based on water potential equilibrium between the guard cells and the air at the bottom of the stomatal pore and contains three independent variables: gs(0), Z and ?. gs(0) is the value of stomatal conductance that would occur at saturating humidity and will vary among leaves and with CO2 and light. The value of Z is determined primarily by the resistance to heat transfer from the epidermis to the evaporating site and the value of ? is determined primarily by the resistance to water vapour diffusion from the evaporating site to the guard cells. This leads to the two predictions that were tested. Firstly, the values of Z and ? should be constant for leaves of a given species grown under given conditions, although gs(0) should vary among leaves and with light and CO2. And secondly, the ratio of Z to ? should be higher in leaves having their stomata in crypts because the distance for heat transfer is greater than that for water vapour diffusion. Data from three species, Nerium oleander, Pastinaca sativum and Xanthium strumarium support these two predictions. PMID:23072325
Chen, Yong-Hua; Wu, Xiao-Fu; Hao, Jun; Chen, Ming-Li; Zhu, Guang-Yu
In order to solve the problem that wetland herbaceous plants tend to die during winter in subtropics areas, selection and purification potential evaluation experiments were carried out by introducing into the constructed wetlands 16 species of woody wetland plants. Cluster analysis was performed by including the morphological characteristics, physiological characteristics, as well as nitrogen and phosphorus accumulation of the woody wetland plants. The results indicated that there were significant differences among the tested woody plants in their survival rate, height increase, root length increase and vigor, Chlorophyll content, Superoxide dismutase, Malonaldehyde, Proline, Peroxidase, biomass, average concentration and accumulation of nitrogen and phosphorus. Based on the established evaluation system, the tested plants were clustered into 3 groups. The plants in the 1st group possessing high purification potentials are Nerium oleander and Hibiscus syriacus. Those in the 2nd group possessing moderate purification potentials are Trachycarpus fortune, Llex latifolia Thunb., Gardenia jasminoides, Serissa foetida and Ilex crenatacv Convexa. And those in the 3rd group with low purification potentials are Jasminum udiflorum, Hedera helix, Ligustrum vicaryi, Ligustrum lucidum, Buxus sempervives, Murraya paniculata, Osmanthus fragrans, Mahoniafortune and Photinia serrulata. PMID:24812951
Qasem, Jamal R
A field survey was carried out to record plant species climbed by Ephedra alte in certain parts of Jordan during 2008-2010. Forty species of shrubs, ornamental, fruit, and forest trees belonging to 24 plant families suffered from the climbing habit of E. alte. Growth of host plants was adversely affected by E. alte growth that extended over their vegetation. In addition to its possible competition for water and nutrients, the extensive growth it forms over host species prevents photosynthesis, smothers growth and makes plants die underneath the extensive cover. However, E. alte did not climb all plant species, indicating a host preference range. Damaged fruit trees included Amygdalus communis, Citrus aurantifolia, Ficus carica, Olea europaea, Opuntia ficus-indica, and Punica granatum. Forestry species that were adversely affected included Acacia cyanophylla, Ceratonia siliqua, Crataegus azarolus, Cupressus sempervirens, Pinus halepensis, Pistacia atlantica, Pistacia palaestina, Quercus coccifera, Quercus infectoria, Retama raetam, Rhamnus palaestina, Rhus tripartita, and Zizyphus spina-christi. Woody ornamentals attacked were Ailanthus altissima, Hedera helix, Jasminum fruticans, Jasminum grandiflorum, Nerium oleander, and Pyracantha coccinea. Results indicated that E. alte is a strong competitive for light and can completely smother plants supporting its growth. A. communis, F. carica, R. palaestina, and C. azarolus were most frequently attacked. PMID:22645486
Qasem, Jamal R.
A field survey was carried out to record plant species climbed by Ephedra alte in certain parts of Jordan during 2008–2010. Forty species of shrubs, ornamental, fruit, and forest trees belonging to 24 plant families suffered from the climbing habit of E. alte. Growth of host plants was adversely affected by E. alte growth that extended over their vegetation. In addition to its possible competition for water and nutrients, the extensive growth it forms over host species prevents photosynthesis, smothers growth and makes plants die underneath the extensive cover. However, E. alte did not climb all plant species, indicating a host preference range. Damaged fruit trees included Amygdalus communis, Citrus aurantifolia, Ficus carica, Olea europaea, Opuntia ficus-indica, and Punica granatum. Forestry species that were adversely affected included Acacia cyanophylla, Ceratonia siliqua, Crataegus azarolus, Cupressus sempervirens, Pinus halepensis, Pistacia atlantica, Pistacia palaestina, Quercus coccifera, Quercus infectoria, Retama raetam, Rhamnus palaestina, Rhus tripartita, and Zizyphus spina-christi. Woody ornamentals attacked were Ailanthus altissima, Hedera helix, Jasminum fruticans, Jasminum grandiflorum, Nerium oleander, and Pyracantha coccinea. Results indicated that E. alte is a strong competitive for light and can completely smother plants supporting its growth. A. communis, F. carica, R. palaestina, and C. azarolus were most frequently attacked. PMID:22645486
Van Kanegan, Michael J; He, Dong Ning; Dunn, Denise E; Yang, Peiying; Newman, Robert A; West, Anne E; Lo, Donald C
We have previously shown that the botanical drug candidate PBI-05204, a supercritical CO2 extract of Nerium oleander, provides neuroprotection in both in vitro and in vivo brain slice-based models for focal ischemia (Dunn et al., 2011). Intriguingly, plasma levels of the neurotrophin BDNF were increased in patients treated with PBI-05204 in a phase I clinical trial (Bidyasar et al., 2009). We thus tested the hypothesis that neuroprotection provided by PBI-05204 to rat brain slices damaged by oxygen-glucose deprivation (OGD) is mediated by BDNF. We found, in fact, that exogenous BDNF protein itself is sufficient to protect brain slices against OGD, whereas downstream activation of TrkB receptors for BDNF is necessary for neuroprotection provided by PBI-05204, using three independent methods. Finally, we provide evidence that oleandrin, the principal cardiac glycoside component of PBI-05204, can quantitatively account for regulation of BDNF at both the protein and transcriptional levels. Together, these findings support further investigation of cardiac glycosides in providing neuroprotection in the context of ischemic stroke. PMID:24431454
Isaac, G S; Abu-Tahon, M A
Medicinal plant extracts of five plants; Adhatoda vasica, Eucalyptus globulus, Lantana camara, Nerium oleander and Ocimum basilicum collected from Cairo, Egypt were evaluated against Fusarium oxysporum f. sp. lycopersici race 3 in vitro conditions using water and certain organic solvents. The results revealed that cold distilled water extracts of O. basilicum and E. globulus were the most effective ones for inhibiting the growth of F. oxysporum f. sp. lycopersici. Butanolic and ethanolic extracts of the tested plants inhibited the pathogen growth to a higher extent than water extracts. Butanolic extract of O. basilicum completely inhibited the growth of F. oxysporum f. sp. lycopersici at concentrations 1.5 and 2.0% (v/v). Butanolic extracts (2.0%) of tested plants had a strong inhibitory effect on hydrolytic enzymes; ?-glucosidase, pectin lyase and protease of F. oxysporum f. sp. lycopersici. This study has confirmed that the application of plant extracts, especially from O. basilicum for controlling F. oxysporum f. sp. lycopersici is environmentally safe, cost effective and does not disturb ecological balance. Investigations are in progress to test the efficacy of O. basilicum extract under in vivo conditions. PMID:24561899
Harissis, Haralampos V
It is known from ancient sources that "laurel," identified with sweet bay, was used at the ancient Greek oracle of Delphi. The Pythia, the priestess who spoke the prophecies, purportedly used laurel as a means to inspire her divine frenzy. However, the clinical symptoms of the Pythia, as described in ancient sources, cannot be attributed to the use of sweet bay, which is harmless. A review of contemporary toxicological literature indicates that it is oleander that causes symptoms similar to those of the Pythia, while a closer examination of ancient literary texts indicates that oleander was often included under the generic term laurel. It is therefore likely that it was oleander, not sweet bay, that the Pythia used before the oracular procedure. This explanation could also shed light on other ancient accounts regarding the alleged spirit and chasm of Delphi, accounts that have been the subject of intense debate and interdisciplinary research for the last hundred years. PMID:25959349
Michael Wink; Dietrich Schneider
Summary Larvae of three moth species were compared with respect to strategies used to cope with secondary metabolites (allelochemicals) present in their diet.Syntomeida epilais is monophagous and accepted only oleander (which contains cardenolides, CG). CG were detected as stored products in the larvae and also in the faeces and exuviae. Pure CG (digoxin and gitoxin) which do not occur in
Hong Lin; Edwin L. Civerolo; Rong Hu; Samuel Barros; Marta Francis; M. Andrew Walker
A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X.
Doni, S; Macci, C; Peruzzi, E; Iannelli, R; Ceccanti, B; Masciandaro, G
The continuous stream of sediments, dredged from harbors and waterways for keeping shipping traffic efficiency, is a considerable ongoing problem recognized worldwide. This problem gets worse as most of the sediments dredged from commercial ports and waterways turn out to be polluted by a wide range of organic and inorganic contaminants. In this study, phytoremediation was explored as a sustainable reclamation technology for turning slightly-polluted brackish dredged sediments into a matrix feasible for productive use. To test this possibility, a phytoremediation experimentation was carried out in containers of about 0.7 m(3) each, filled with brackish dredged sediments contaminated by heavy metals and hydrocarbons. The sediments were pre-conditioned by adding an agronomic soil (30 % v/v) to improve their clayey granulometric composition, and by topping the mixture with high quality compost (4 kg m(-2)) to favour the initial adaptation of the selected vegetal species. The following plant treatments were tested: (1) Paspalum vaginatum, (2) Phragmites australis, (3) Spartium junceum + P. vaginatum, (4) Nerium oleander + P. vaginatum, (5) Tamarix gallica + P. vaginatum, and (6) unplanted control. Eighteen months after the beginning of the experimentation, all the plant species were found in healthy condition and well developed. Throughout the whole experiment, the monitored biological parameters (total microbial population and dehydrogenase activity) were generally observed as constantly increasing in all the planted sediments more than in the control, pointing out an improvement of the chemico-physical conditions of both microorganisms and plants. The concentration decrease of organic and inorganic contaminants (>35 and 20 %, respectively) in the treatments with plants, particularly in the T. gallica + P. vaginatum, confirmed the importance of the root-microorganism interaction in activating the decontamination processes. Finally, the healthy state of the plants and the sediment characteristics, approaching those of an uncontaminated natural soil (technosoil), indicated the efficiency and success of this technology for brackish sediments reclamation. PMID:23183938
Two main research questions are framing this investigation: (1) the main taxa of the medicinal importance value altered the Showbak forest stand and species composition? (2) The most safe species and what are the toxic ones (unsafe). These two research questions are the vital ones to draw a clear image about the wild medicinal plants of this investigated area of Showbak region in Jordan. 79 wild medicinal plant species were investigated in this study which are used in traditional medication for the treatment of various diseases. Most of the locals interviewed dealt with well-known safe medicinal plants such as Aaronsohnia factorovskyi Warb. et Eig., Achillea santolina L., Adiantum capillus-veneris L., Artemisia herba-alba L., Ceratonia siliqua L., Clematis recta L., Herniaria hirsuta L., Malva neglecta Wallr., Rosmarinus officinalis L., Ruta chalepensis L., Salvia triloba L., Sarcopoterium spinosa (L.) Spach., Thymbra capitata (L.) Hof, and Urginea maritima Barker. Many of the wild medicinal plants investigated were toxic and needed to be practiced by practitioners and herbalists rather than the local healers. These plants include Calotropis procera Willd R.Br., Citrullus colocynthis (L.) Sch., Datura stramonium L., Digitalis purpurea L., Ecballium elaterium (L.) A.Rich., Euphorbia helioscopia L., Euphorbia tinctoria Boiss., Glaucium corniculatum (L.) Curt., Hyoscyamus aureus L., Mandragora officinarum L., Nerium oleander L., Ricinus communis L., Solanum nigrum L., Withania somnifera (L.) Dunel. The conservation of medicinal plants and natural resources is becoming increasingly important, so this research is trying to collect information from local population concerning the use of medicinal plants in Showbak; identify the most important specie; determine the relative importance value of the species and calculate the informant consensus factor (ICF) for the medicinal plants. Obtaining results is relied on the interviewee's personal information and the medicinal use of specific plants. PMID:19429338
Tahraoui, A; El-Hilaly, J; Israili, Z H; Lyoussi, B
This survey was undertaken in the Errachidia province in south-eastern Morocco in order to inventory the main medicinal plants used in folk medicine to treat arterial hypertension and diabetes mellitus. Four hundred individuals who knew about and/or had used the medicinal plants for the indicated diseases, including some herbal healers, were interviewed throughout different regions of the province. The inventory of medicinal plants is summarized in a synoptic table, which contains the scientific, vernacular and common name of the plant, its ecological distribution, the part of the plant and the preparation used and the therapeutic indication. Extensive investigations have brought to light 64 medicinal plants belonging to 33 families; of these, 45 are used for diabetes, 36 for hypertension, and 18 for both diseases. Of these plants, 34% grow in the wild, 44% are cultivated, and 22% are not indigenous to the area and are brought from other parts of Morocco or from outside the country. The survey shows that 78% of the patients regularly use these medicinal plants. In this region, the most frequently used plants to treat diabetes include Ajuga iva, Allium cepa, Artemisia herba-alba, Carum carvi, Lepidium sativum, Nigella sativa, Olea europaea, Peganum harmala, Phoenix dactylifera, Rosmarinus officinalis, and Zygophyllum gaetulum, and those to treat hypertension include Ajuga iva, Allium cepa, Allium sativum, Artemisia herba-alba Asso, Carum carvi, Nigella sativa, Olea europea, Rosmarinus officinalis, Origanum majorana, Peganum harmala, and Phoenix dactylifera. The local people recognize the toxic plants and are very careful in using such plants, which are Citrullus colocynthis, Datura stramonium, Nerium oleander, Nigella sativa, Peganum harmala and Zygophyllum gaetulum. Our survey shows that traditional medicine in the south-eastern Moroccan population has not only survived but has thrived in the transcultural environment and intermixture of many ethnic traditions and beliefs. PMID:17052873
Optical spectroscopy, a truly non-invasive tool for remote diagnostics, is capable of providing valuable information on the structure and function of molecules. However, most spectroscopic techniques suffer from drawbacks, which limit their application. As a part of my dissertation work, I have developed theoretical and experimental methods to address the above mentioned issues. I have successfully applied these methods for monitoring the physical, chemical and biochemical parameters of biomolecules involved in some specific life threatening diseases like lead poisoning and age-related macular degeneration (AMD). I presented optical studies of melanosomes, which are one of the vital organelles in the human eye, also known to be responsible for a disease called age-related macular degeneration (AMD), a condition of advanced degeneration which causes progressive blindness. I used Raman spectroscopy, to first chemically identify the composition of melanosome, and then monitor the changes in its functional and chemical behavior due to long term exposure to visible light. The above study, apart from explaining the role of melanosomes in AMD, also sets the threshold power for lasers used in surgeries and other clinical applications. In the second part of my dissertation, a battery of spectroscopic techniques was successfully applied to explore the different binding sites of lead ions with the most abundant carrier protein molecule in our circulatory system, human serum albumin. I applied optical spectroscopic tools for ultrasensitive detection of heavy metal ions in solution which can also be used for lead detection at a very early stage of lead poisoning. Apart from this, I used Raman microspectroscopy to study the chemical alteration occurring inside a prostate cancer cell as a result of a treatment with a low concentrated aqueous extract of a prospective drug, Nerium Oleander. The experimental methods used in this study has tremendous potential for clinical application and will gain widespread acceptance within next few years from bench to bedside as an inexpensive and non-invasive tool compared to the other technologies.
Alexandra K. Shibata; Françoise M. Robert
The effect of plants (milo, oleander and buffelgrass) and hexadecane (1 g\\/kg of soil) on the diversity of hexadecane-degraders\\u000a in a coastal soil was investigated. Hexadecane was rapidly degraded during the first 56 days. Its depletion was not plant-enhanced\\u000a but was slightly retarded by milo and buffelgrass. The diversity of the dominant cultured hexadecane-degrading bacteria was\\u000a based on sequencing of the V6-8
Caralyn B. Zehnder; Mark D. Hunter
Induced plant responses to leaf-chewing insects have been well studied, but considerably less is known about the effects of\\u000a phloem-feedings insects on induction. In a set of laboratory experiments, we examined density-dependent induction by the milkweed-oleander\\u000a aphid, Aphis nerii, of putative defenses in four milkweed species (Asclepias incarnata, Asclepias syriaca, Asclepias tuberosa, and Asclepias viridis). We hypothesized that high aphid
Longenecker, D. E.; Lyerly, P. J.
Blackberry Raspberry Strawberry Boysenberry ~oderately~ Very sensitive2 tolerant oler rant^ tolerant2 Viburnum Field Bean White Dutch Clover Alsike Clover Red Clover Ladino Clover Crimson Clover Burnet Meadow Foxtail Carrot English Pea.... (continued) FRUIT, NUT AND VINE CROPS~ Pecan Pomegranate Date Palm Peach Fig Apricot Olive Grape Quince ORNAMENTAL SHRUBS Spreading Oleander Purple Sage Juniper Arbor Vitae Bottlebrush Lantana Py racantha Privet Japonica Data taken from many...
Metcalfe, Chris; Gunnell, David; Mohamed, Fahim; Eddleston, Michael
Background The absorption, distribution, metabolism and elimination of medicines are partly controlled by transporters and enzymes with diurnal variation in expression. Dose timing may be important for maximizing therapeutic and minimizing adverse effects. However, outcome data for such an effect in humans are sparse, and chronotherapeutics is consequently less practised. We examined a large prospective Sri Lankan cohort of patients with acute poisoning to seek evidence of diurnal variation in the probability of survival. Methods In all, 14?840 patients admitted to hospital after yellow oleander (Cascabela thevetia) seed or pesticide [organophosphorus (OP), carbamate, paraquat, glyphosate] self-poisoning were investigated for variation in survival according to time of ingestion. Results We found strong evidence that the outcome of oleander poisoning was associated with time of ingestion (P?0.001). There was weaker evidence for OP insecticides (P?=?0.041) and no evidence of diurnal variation in the outcome for carbamate, glyphosate and paraquat pesticides. Compared with ingestion in the late morning, and with confounding by age, sex, time of and delay to hospital presentation and year of admission controlled, case fatality of oleander poisoning was over 50% lower following evening ingestion (risk ratio?=?0.40, 95% confidence interval 0.26–0.62). Variation in dose across the day was not responsible. Conclusions We have shown for the first time that timing of poison ingestion affects survival in humans. This evidence for chronotoxicity suggests chronotherapeutics should be given greater attention in drug development and clinical practice. PMID:23179303
Crous, P W; Shivas, R G; Quaedvlieg, W; van der Bank, M; Zhang, Y; Summerell, B A; Guarro, J; Wingfield, M J; Wood, A R; Alfenas, A C; Braun, U; Cano-Lira, J F; García, D; Marin-Felix, Y; Alvarado, P; Andrade, J P; Armengol, J; Assefa, A; den Breeÿen, A; Camele, I; Cheewangkoon, R; De Souza, J T; Duong, T A; Esteve-Raventós, F; Fournier, J; Frisullo, S; García-Jiménez, J; Gardiennet, A; Gené, J; Hernández-Restrepo, M; Hirooka, Y; Hospenthal, D R; King, A; Lechat, C; Lombard, L; Mang, S M; Marbach, P A S; Marincowitz, S; Marin-Felix, Y; Montaño-Mata, N J; Moreno, G; Perez, C A; Pérez Sierra, A M; Robertson, J L; Roux, J; Rubio, E; Schumacher, R K; Stchigel, A M; Sutton, D A; Tan, Y P; Thompson, E H; van der Linde, E; Walker, A K; Walker, D M; Wickes, B L; Wong, P T W; Groenewald, J Z
Novel species of microfungi described in the present study include the following from South Africa: Cercosporella dolichandrae from Dolichandra unguiscati, Seiridium podocarpi from Podocarpus latifolius, Pseudocercospora parapseudarthriae from Pseudarthria hookeri, Neodevriesia coryneliae from Corynelia uberata on leaves of Afrocarpus falcatus, Ramichloridium eucleae from Euclea undulata and Stachybotrys aloeticola from Aloe sp. (South Africa), as novel member of the Stachybotriaceae fam. nov. Several species were also described from Zambia, and these include Chaetomella zambiensis on unknown Fabaceae, Schizoparme pseudogranati from Terminalia stuhlmannii, Diaporthe isoberliniae from Isoberlinia angolensis, Peyronellaea combreti from Combretum mossambiciensis, Zasmidium rothmanniae and Phaeococcomyces rothmanniae from Rothmannia engleriana, Diaporthe vangueriae from Vangueria infausta and Diaporthe parapterocarpi from Pterocarpus brenanii. Novel species from the Netherlands include: Stagonospora trichophoricola, Keissleriella trichophoricola and Dinemasporium trichophoricola from Trichophorum cespitosum, Phaeosphaeria poae, Keissleriella poagena, Phaeosphaeria poagena, Parastagonospora poagena and Pyrenochaetopsis poae from Poa sp., Septoriella oudemansii from Phragmites australis and Dendryphion europaeum from Hedera helix (Germany) and Heracleum sphondylium (the Netherlands). Novel species from Australia include: Anungitea eucalyptorum from Eucalyptus leaf litter, Beltraniopsis neolitseae and Acrodontium neolitseae from Neolitsea australiensis, Beltraniella endiandrae from Endiandra introrsa, Phaeophleospora parsoniae from Parsonia straminea, Penicillifer martinii from Cynodon dactylon, Ochroconis macrozamiae from Macrozamia leaf litter, Triposporium cycadicola, Circinotrichum cycadis, Cladosporium cycadicola and Acrocalymma cycadis from Cycas spp. Furthermore, Vermiculariopsiella dichapetali is described from Dichapetalum rhodesicum (Botswana), Ophiognomonia acadiensis from Picea rubens (Canada), Setophoma vernoniae from Vernonia polyanthes and Penicillium restingae from soil (Brazil), Pseudolachnella guaviyunis from Myrcianthes pungens (Uruguay) and Pseudocercospora neriicola from Nerium oleander (Italy). Novelties from Spain include: Dendryphiella eucalyptorum from Eucalyptus globulus, Conioscypha minutispora from dead wood, Diplogelasinospora moalensis and Pseudoneurospora canariensis from soil and Inocybe lanatopurpurea from reforested woodland of Pinus spp. Novelties from France include: Kellermania triseptata from Agave angustifolia, Zetiasplozna acaciae from Acacia melanoxylon, Pyrenochaeta pinicola from Pinus sp. and Pseudonectria rusci from Ruscus aculeatus. New species from China include: Dematiocladium celtidicola from Celtis bungeana, Beltrania pseudorhombica, Chaetopsina beijingensis and Toxicocladosporium pini from Pinus spp. and Setophaeosphaeria badalingensis from Hemerocallis fulva. Novel genera of Ascomycetes include Alfaria from Cyperus esculentus (Spain), Rinaldiella from a contaminated human lesion (Georgia), Hyalocladosporiella from Tectona grandis (Brazil), Pseudoacremonium from Saccharum spontaneum and Melnikomyces from leaf litter (Vietnam), Annellosympodiella from Juniperus procera (Ethiopia), Neoceratosperma from Eucalyptus leaves (Thailand), Ramopenidiella from Cycas calcicola (Australia), Cephalotrichiella from air in the Netherlands, Neocamarosporium from Mesembryanthemum sp. and Acervuloseptoria from Ziziphus mucronata (South Africa) and Setophaeosphaeria from Hemerocallis fulva (China). Several novel combinations are also introduced, namely for Phaeosphaeria setosa as Setophaeosphaeria setosa, Phoma heteroderae as Peyronellaea heteroderae and Phyllosticta maydis as Peyronellaea maydis. Morphological and culture characteristics along with ITS DNA barcodes are provided for all taxa. PMID:25264390
Recent studies reported weakening in the Atlantic Meridional Overturning Circulation (AMOC) and in the Gulf Stream (GS), using records of about a decade (RAPID project) or two (altimeter data). Coastal sea level records are much longer, so the possibility of detecting climatic changes in ocean circulation from sea level data is intriguing and thus been examined here. First, it is shown that variations in the AMOC transport from the RAPID project since 2004 are consistent with the flow between Bermuda and the U. S. coast derived from the Oleander measurements and from sea level difference (SLDIF). Despite apparent disagreement between recent studies on the ability of data to detect weakening in the GS flow, estimated transport changes from 3 different independent data sources agree quite well with each other on the extreme decline in transport in 2009-2010. Due to eddies and meandering, the flow representing the GS part of the Oleander line is not correlated with AMOC or with the Florida Current, only the flow across the entire Oleander line from the U.S. coast to Bermuda is correlated with climatic transport changes. Second, Empirical Mode Decomposition (EMD) analysis shows that SLDIF can detect (with lag) the portion of the variations in the AMOC transport that are associated with the Florida Current and the wind-driven Ekman transport (SLDIF-transport correlations of ~ 0.7-0.9). The SLDIF has thus been used to estimate variations in transport since 1935 and compared with AMOC obtained from reanalysis data. The significant weakening in AMOC after ~ 2000 (~ 4.5 Sv per decade) is comparable to weakening seen in the 1960s to early 1970s. Both periods of weakening AMOC, in the 1960s and 2000s, are characterized by faster than normal sea level rise along the northeastern U.S. coast, so monitoring changes in AMOC has practical implications for coastal protection.
Francisco F. Roberto; Harry Klee; Frank White; Russell Nordeen; Tsune Kosuge
The gene encoding N^?-(indole-3-acetyl)-L-lysine synthetase, iaaL, from Pseudomonas savastanoi was localized within a 4.25-kilobase EcoRI fragment derived from pIAA1 of oleander strain EW 2009. Two open reading frames of 606 and 1188 nucleotides were identified upon sequencing, which directed the in vitro synthesis of M_r21,000 and M_r 44,000 proteins. Expression of an open reading frame-2 subclone, pMON686, in Escherichia coli
Durasnel, P; Vanhuffel, L; Blondé, R; Lion, F; Galas, T; Mousset-Hovaere, M; Balaÿ, I; Viscardi, G; Valyi, L
The authors describe three cases of severe accidental poisoning by plants used as part of a traditional treatment in Mayotte. The established, or suspected, toxicity of Thevetia peruviana (Yellow oleander), Cinchona pubescens (Red quinine-tree), Melia azaderach (Persian lilac, also called china berry) and Azadirachta indica (Neem), is discussed. The clinical presentation is cardiac (atrioventricular block) and well known for Thevetia and Cinchona intoxications. Neurological signs and multi-organ failure are found for Azadirachta and Melia. The identification of the plants is never easy, nor is the evidence of their accountability. In the three cases reported, no other cause than the traditional treatment has been found to explain the clinical presentation. The outcome was favorable in all cases. The authors emphasize the difficulties to investigate these accidents, the poor medical knowledge of these practices in tropical areas, and in Mayotte particularly. The need for cooperation with local botanists, familiar with traditional medicine, is also underlined. PMID:25301110
Ferreira, H; Rodrigues Neto, J; Gonçalves, E R; Rosato, Y B
A simplified protocol of subtractive hybridization based on the technique of L. M. Kunkel, A. P. Monaco, W. Middlesworth, H. D. Ochs & S. A. Latt (1985, Proc Natl Acad Sci USA, 82, 4778-4782) was used to obtain DNA sequences specific to Xylella fastidiosa isolated from diseased citrus plants. As a driver, DNA extracted from bacteria showing different degrees of relatedness was used: Xy. fastidiosa 788 isolated from another host (plum), Xanthomonas campestris pv. campestris and Burkholderia gladioli strains. A DNA fragment, f14, showing no hybridization to the driver DNA, was used as a probe specific to Xy. fastidiosa from citrus and oleander. This fragment was sequenced and the predicted protein showed 40% similarity to the central region of flagellin of Escherichia coli serotypes H1 and H12. A pair of internal primers (f14-1 and f14-2) was designed for amplification of Xy. fastidiosa DNA. These primers detected Xy. fastidiosa strains isolated from citrus and oleander and yielded an amplification product of about 600 bp. They were also able to detect the bacteria in extracts from citrus plants with or without symptoms of disease. No amplification reaction was obtained using DNA extracted from other species and pathovars of Xanthomonas, Pseudomonas cichorii, Erwinia carotovora, Agrobacterium tumefaciens and phytopathogens of citrus (Xanthomonas axonopodis pv. citri) and coffee (Burkholderia andropogonis, P. cichorii, Pseudomonas syringae pv. garcae). The isolation of a DNA fragment specific to Xy. fastidiosa from citrus showed that the simplified protocol of subtractive hybridization used in this work is potentially applicable to other micro-organisms. PMID:10463163
Background Pseudomonas savastanoi pv. savastanoi is the causal agent of olive knot disease. The strains isolated from oleander and ash belong to the pathovars nerii and fraxini, respectively. When artificially inoculated, pv. savastanoi causes disease also on ash, and pv. nerii attacks also olive and ash. Surprisingly nothing is known yet about their distribution in nature on these hosts and if spontaneous cross-infections occur. On the other hand sanitary certification programs for olive plants, also including P. savastanoi, were launched in many countries. The aim of this work was to develop several PCR-based tools for the rapid, simultaneous, differential and quantitative detection of these P. savastanoi pathovars, in multiplex and in planta. Results Specific PCR primers and probes for the pathovars savastanoi, nerii and fraxini of P. savastanoi were designed to be used in End Point and Real-Time PCR, both with SYBR® Green or TaqMan® chemistries. The specificity of all these assays was 100%, as assessed by testing forty-four P. savastanoi strains, belonging to the three pathovars and having different geographical origins. For comparison strains from the pathovars phaseolicola and glycinea of P. savastanoi and bacterial epiphytes from P. savastanoi host plants were also assayed, and all of them tested always negative. The analytical detection limits were about 5 - 0.5 pg of pure genomic DNA and about 102 genome equivalents per reaction. Similar analytical thresholds were achieved in Multiplex Real-Time PCR experiments, even on artificially inoculated olive plants. Conclusions Here for the first time a complex of PCR-based assays were developed for the simultaneous discrimination and detection of P. savastanoi pv. savastanoi, pv. nerii and pv. fraxini. These tests were shown to be highly reliable, pathovar-specific, sensitive, rapid and able to quantify these pathogens, both in multiplex reactions and in vivo. Compared with the other methods already available for P. savastanoi, the identification procedures here reported provide a versatile tool both for epidemiological and ecological studies on these pathovars, and for diagnostic procedures monitoring the asymptomatic presence of P. savastanoi on olive and oleander propagation materials. PMID:20509893
PsasM2I, a type II restriction-modification system in Pseudomonas savastanoi pv. savastanoi: differential distribution of carrier strains in the environment and the evolutionary history of homologous RM systems in the Pseudomonas syringae complex.
Cinelli, Tamara; Moscetti, Ilaria; Marchi, Guido
A type II restriction-modification system was found in a native plasmid of Pseudomonas savastanoi pv. savastanoi MLLI2. Functional analysis of the methyltransferase showed that the enzyme acts by protecting the DNA sequence CTGCAG from cleavage. Restriction endonuclease expression in recombinant Escherichia coli cells resulted in mutations in the REase sequence or transposition of insertion sequence 1A in the coding sequence, preventing lethal gene expression. Population screening detected homologous RM systems in other P. savastanoi strains and in the Pseudomonas syringae complex. An epidemiological survey carried out by sampling olive and oleander knots in two Italian regions showed an uneven diffusion of carrier strains, whose presence could be related to a selective advantage in maintaining the RM system in particular environments or subpopulations. Moreover, carrier strains can coexist in the same orchards, plants, and knot tissues with non-carriers, revealing unexpected genetic variability on a very small spatial scale. Phylogenetic analysis of the RM system and housekeeping gene sequences in the P. syringae complex demonstrated the ancient acquisition of the RM systems. However, the evolutionary history of the gene complex also showed the involvement of horizontal gene transfer between related strains and recombination events. PMID:25008981
Woods, L W; Filigenzi, M S; Booth, M C; Rodger, L D; Arnold, J S; Puschner, B
Three horses died as a result of eating grass hay containing summer pheasant's eye (Adonis aestivalis L.), a plant containing cardenolides similar to oleander and foxglove. A 9-year-old thoroughbred gelding, a 20-year-old appaloosa gelding, and a 5-year-old quarter horse gelding initially presented with signs of colic 24-48 hours after first exposure to the hay. Gastrointestinal gaseous distension was the primary finding on clinical examination of all three horses. Two horses became moribund and were euthanatized 1 day after first showing clinical signs, and the third horse was euthanatized after 4 days of medical therapy. Endocardial hemorrhage and gaseous distension of the gastrointestinal tract were the only necropsy findings in the first two horses. On microscopic examination, both horses had scattered foci of mild, acute myocardial necrosis and neutrophilic inflammation associated with endocardial and epicardial hemorrhage. The third horse that survived for 4 days had multifocal to coalescing, irregular foci of acute, subacute, and chronic myocardial degeneration and necrosis. A. aestivalis (pheasant's eye, summer adonis) was identified in the hay. Strophanthidin, the aglycone of several cardenolides present in Adonis spp., was detected by liquid chromatography-mass spectrometry-mass spectrometry in gastrointestinal contents from all three horses. Although Adonis spp. contain cardiac glycosides, cardiac lesions have not previously been described in livestock associated with consumption of adonis, and this is the first report of adonis toxicosis in North America. PMID:15133169
Comai, L; Kosuge, T
Olive (or oleander) knot is a plant disease incited by Pseudomonas savastanoi. Disease symptoms consist of tumorous outgrowths induced in the plant by bacterial production of indole-3-acetic acid (IAA). Synthesis of IAA occurs by the following reactions: L-tryptophan leads to indoleacetamide leads to indoleacetic acid, catalyzed by tryptophan 2-monooxygenase and indoleacetamide hydrolase, respectively. Whereas the enzymology of IAA synthesis is well characterized, nothing is known about the genetics of the system. We devised a positive selection for the presence of tryptophan 2-monooxygenase based on its capacity to use as a substrate the toxic tryptophan analogue 5-methyltryptophan. Efficient curing of the bacterium of tryptophan 2-monoxygenase, indoleacetamide hydrolase, and IAA production was obtained by acridine orange treatment. Further, loss of capacity to produce IAA by curing was correlated with loss of a plasmid of 34 X 10(6) molecular weight. This plasmid, here called pIAA1, when reintroduced into Iaa- mutants by transformation, restored tryptophan 2-monooxygenase and indoleacetamide hydrolase activities and production of IAA. Images PMID:7204339
Zehnder, Caralyn B; Hunter, Mark D
Induced plant responses to leaf-chewing insects have been well studied, but considerably less is known about the effects of phloem-feedings insects on induction. In a set of laboratory experiments, we examined density-dependent induction by the milkweed-oleander aphid, Aphis nerii, of putative defenses in four milkweed species (Asclepias incarnata, Asclepias syriaca, Asclepias tuberosa, and Asclepias viridis). We hypothesized that high aphid density would lead to increased cardenolide expression in species with low constitutive levels of cardenolides (e.g., A. tuberosa), but that there would be no induction in high constitutive cardenolide species (e.g., A. viridis). Based on previous studies, we did not expect cardenolide induction in A. incarnata. Contrary to our predictions, we observed feeding-induced declines of cardenolide concentrations in A. viridis. Cardenolide concentrations did not respond to aphid feeding in the other three milkweed species. Aphids also caused reductions in biomass accumulation by two of four Asclepias species, A. viridis and A. incarnata. High aphid density led to a decrease in A. viridis foliar nitrogen concentration. However, aphids had no effect on the defensive chemistry, growth, or nutritional quality of either A. syriaca or A. tuberosa. Our results highlight that congeneric plant species may respond differently to the same levels of herbivore damage. PMID:17929096
Wrightia tinctoria R. Br. belongs to family Apocynaceae commonly called as Sweet Indrajao, Pala Indigo Plant, Dyer's Oleander. “Jaundice curative tree” in south India. Sweet Indrajao is a small, deciduous tree with a light gray, scaly smooth bark. Native to India and Burma, Wrightia is named after a Scottish physician and botanist William Wright (1740-1827). Sweet Indrajao is called dhudi (Hindi) because of its preservative nature. The juice of the tender leaves is used efficaciously in jaundice. Crushed fresh leaves when filled in the cavity of decayed tooth relieve toothache. In Siddha system of medicine, it is used for psoriasis and other skin diseases. Oil 777 prepared out of the fresh leaves of the plant has been assigned to analgesic, anti-inflammatory, and anti-pyretic activities and to be effective in the treatment of psoriasis. The plant is reported to contain presence of flavanoid, glycoflavones-iso-orientin, and phenolic acids. The various chemical constituents isolated from various parts of the plant are reported as 3,4-Seco-lup-20 (29)-en-3-oic acid, lupeol, stigmasterol and campetosterol, Indigotin, indirubin, tryptanthrin, isatin, anthranillate and rutin Triacontanol, Wrightial, cycloartenone, cycloeucalenol, ?-amyrin, Alpha-Amyrin, and ?-sitosterol, 14?-methylzymosterol. Four uncommon sterols, desmosterol, clerosterol, 24-methylene-25-methylcholesterol, and 24-dehydropollinastanol, were isolated and identified in addition to several more common phytosterols. The Triterpinoids components of the leaves and pods of Wrightia tinctoria also isolated. This article intends to provide an overview of the chemical constituents present in various parts of the plants and their pharmacological actions and pharmacognostical evaluation. PMID:24600194
The prevalent way aphids accomplish colony defense against natural enemies is a mutualistic relationship with ants or the occurrence of a specialised soldier caste typical for eusocial aphids, or even both. Despite a group-living life style of those aphid species lacking these defense lines, communal defense against natural predators has not yet been observed there. Individuals of Aphis nerii (Oleander aphid) and Uroleucon hypochoeridis, an aphid species feeding on Hypochoeris radicata (hairy cat's ear), show a behavioral response to visual stimulation in the form of spinning or twitching, which is often accompanied by coordinated kicks executed with hind legs. Interestingly, this behaviour is highly synchronized among members of a colony and repetitive visual stimulation caused strong habituation. Observations of natural aphid colonies revealed that a collective twitching and kicking response (CTKR) was frequently evoked during oviposition attempts of the parasitoid wasp Aphidius colemani and during attacks of aphidophagous larvae. CTKR effectively interrupted oviposition attempts of this parasitoid wasp and even repelled this parasitoid from colonies after evoking consecutive CTKRs. In contrast, solitary feeding A. nerii individuals were not able to successfully repel this parasitoid wasp. In addition, CTKR was also evoked through gentle substrate vibrations. Laser vibrometry of the substrate revealed twitching-associated vibrations that form a train of sharp acceleration peaks in the course of a CTKR. This suggests that visual signals in combination with twitching-related substrate vibrations may play an important role in synchronising defense among members of a colony. In both aphid species collective defense in encounters with different natural enemies was executed in a stereotypical way and was similar to CTKR evoked through visual stimulation. This cooperative defense behavior provides an example of a surprising sociality that can be found in some aphid species that are not expected to be social at all. PMID:20454683
Morgan, J. Kent; Luzio, Gary A.; Ammar, El-Desouky; Hunter, Wayne B.; Hall, David G.; Shatters Jr, Robert G.
Stylet sheath formation is a common feature among phytophagous hemipterans. These sheaths are considered essential to promote a successful feeding event. Stylet sheath compositions are largely unknown and their mode of solidification remains to be elucidated. This report demonstrates the formation and solidification of in ?ere (in air) produced stylet sheaths by six hemipteran families: Diaphorina citri (Psyllidae, Asian citrus psyllid), Aphis nerii (Aphididae, oleander/milkweed aphid), Toxoptera citricida (Aphididae, brown citrus aphid), Aphis gossypii (Aphididae, cotton melon aphid), Bemisia tabaci biotype B (Aleyrodidae, whitefly), Homalodisca vitripennis (Cicadellidae, glassy-winged sharpshooter), Ferrisia virgata (Pseudococcidae, striped mealybug), and Protopulvinaria pyriformis (Coccidae, pyriform scale). Examination of in ?ere produced stylet sheaths by confocal and scanning electron microscopy shows a common morphology of an initial flange laid down on the surface of the membrane followed by continuous hollow core structures with sequentially stacked hardened bulbous droplets. Single and multi-branched sheaths were common, whereas mealybug and scale insects typically produced multi-branched sheaths. Micrographs of the in ?ere formed flanges indicate flange sealing upon stylet bundle extraction in D. citri and the aphids, while the B. tabaci whitefly and H. vitripennis glassy-winged sharpshooter flanges remain unsealed. Structural similarity of in ?ere sheaths are apparent in stylet sheaths formed in planta, in artificial diets, or in water. The use of ‘Solvy’, a dissolvable membrane, for intact stylet sheath isolation is reported. These observations illustrate for the first time this mode of stylet sheath synthesis adding to the understanding of stylet sheath formation in phytophagous hemipterans and providing tools for future use in structural and compositional analysis. PMID:23638086
Wrightia tinctoria R. Br. belongs to family Apocynaceae commonly called as Sweet Indrajao, Pala Indigo Plant, Dyer's Oleander. "Jaundice curative tree" in south India. Sweet Indrajao is a small, deciduous tree with a light gray, scaly smooth bark. Native to India and Burma, Wrightia is named after a Scottish physician and botanist William Wright (1740-1827). Sweet Indrajao is called dhudi (Hindi) because of its preservative nature. The juice of the tender leaves is used efficaciously in jaundice. Crushed fresh leaves when filled in the cavity of decayed tooth relieve toothache. In Siddha system of medicine, it is used for psoriasis and other skin diseases. Oil 777 prepared out of the fresh leaves of the plant has been assigned to analgesic, anti-inflammatory, and anti-pyretic activities and to be effective in the treatment of psoriasis. The plant is reported to contain presence of flavanoid, glycoflavones-iso-orientin, and phenolic acids. The various chemical constituents isolated from various parts of the plant are reported as 3,4-Seco-lup-20 (29)-en-3-oic acid, lupeol, stigmasterol and campetosterol, Indigotin, indirubin, tryptanthrin, isatin, anthranillate and rutin Triacontanol, Wrightial, cycloartenone, cycloeucalenol, ?-amyrin, Alpha-Amyrin, and ?-sitosterol, 14?-methylzymosterol. Four uncommon sterols, desmosterol, clerosterol, 24-methylene-25-methylcholesterol, and 24-dehydropollinastanol, were isolated and identified in addition to several more common phytosterols. The Triterpinoids components of the leaves and pods of Wrightia tinctoria also isolated. This article intends to provide an overview of the chemical constituents present in various parts of the plants and their pharmacological actions and pharmacognostical evaluation. PMID:24600194
Martel, John W; Malcolm, Stephen B
The effect of aphid population size on host-plant chemical defense expression and the effect of plant defense on aphid population dynamics were investigated in a milkweed-specialist herbivore system. Density effects of the aposematic oleander aphid, Aphis nerii, on cardenolide expression were measured in two milkweed species, Asclepias curassavica and A. incarnata. These plants vary in constitutive chemical investment with high mean cardenolide concentration in A. curassavica and low to zero in A. incarnata. The second objective was to determine whether cardenolide expression in these two host plants impacts mean A. nerii colony biomass (mg) and density. Cardenolide concentration (microgram/g) of A. curassavica in both aphid-treated leaves and opposite, herbivore-free leaves decreased initially in comparison with aphid-free controls, and then increased significantly with A. nerii density. Thus, A. curassavica responds to aphid herbivory initially with density-dependent phytochemical reduction, followed by induction of cardenolides to concentrations above aphid-free controls. In addition, mean cardenolide concentration of aphid-treated leaves was significantly higher than that of opposite, herbivore-free leaves. Therefore, A. curassavica induction is strongest in herbivore-damage tissue. Conversely, A. incarnata exhibited no such chemical response to aphid herbivory. Furthermore, neither host plant responded chemically to herbivore feeding duration time (days) or to the interaction between herbivore initial density and feeding duration time. There were also no significant differences in mean colony biomass or population density of A. nerii reared on high cardenolide (A. curassavica) and low cardenolide (A. incarnata) hosts. PMID:15139307
Eddleston, Michael; Sudarshan, K.; Senthilkumaran, M.; Reginald, K.; Karalliedde, Lakshman; Senarathna, Lalith; de Silva, Dhammika; Rezvi Sheriff, M. H.; Buckley, Nick A.; Gunnell, David
OBJECTIVES: Most data on self-poisoning in rural Asia have come from secondary hospitals. We aimed to: assess how transfers from primary to secondary hospitals affected estimates of case-fatality ratio (CFR); determine whether there was referral bias according to gender or poison; and estimate the annual incidence of all self-poisoning, and of fatal self-poisoning, in a rural developing-world setting. METHODS: Self-poisoning patients admitted to Anuradhapura General Hospital, Sri Lanka, were reviewed on admission from 1 July to 31 December 2002. We audited medical notes of self-poisoning patients admitted to 17 of the 34 surrounding peripheral hospitals for the same period. FINDINGS: A total of 742 patients were admitted with self-poisoning to the secondary hospital; 81 died (CFR 10.9%). 483 patients were admitted to 17 surrounding peripheral hospitals. Six patients (1.2%) died in peripheral hospitals, 249 were discharged home, and 228 were transferred to the secondary hospital. There was no effect of gender or age on likelihood of transfer; however, patients who had ingested oleander or paraquat were more likely to be transferred than were patients who had taken organophosphorus pesticides or other poisons. Estimated annual incidences of self-poisoning and fatal self-poisoning were 363 and 27 per 100,000 population, respectively, with an overall CFR of 7.4% (95% confidence interval 6.0-9.0). CONCLUSION: Fifty per cent of patients admitted to peripheral hospitals were discharged home, showing that CFRs based on secondary hospital data are inflated. However, while incidence of self-poisoning is similar to that in England, fatal self-poisoning is three times more common in Sri Lanka than fatal self-harm by all methods in England. Population based data are essential for making international comparisons of case fatality and incidence, and for assessing public health interventions. PMID:16628300
Handmann, Patricia; Fischer, Jürgen; Visbeck, Martin; Behrens, Erik; Patara, Lavinia
Time series of observed deep circulation transports and water mass properties in the subpolar North Atlantic are beginning to be long enough to investigate multiannual to decadal variability of the deep water. At the same time high resolution ocean circulation models (1/20° resolution VIKING20 model) can be used to compare observations with model simulation. The models also allow to diagnose the deep water circulation processes more completely and to relate local to basin scale signals. North Atlantic Deep Water (NADW) is a complex combination of water masses from different origins and pathways that meet at the exit of the Labrador Sea. The lower part of NADW is formed by water masses entering the subpolar basin over the Greenland-Scotland ridge. Iceland-Scotland Overflow Water (ISOW) from the eastern sills has the longest pathway and joins the densest deep water component from Denmark Strait (DSOW) after crossing the Mid-Atlantic-Ridge through Charlie-Gibbs Fracture Zone (CGFZ); together, they form the Lower NADW. The upper component of the NADW is composed of Labrador Sea Water (LSW), which is formed and modified through deep convection in the Labrador Sea. Using 60 year long time series of North Atlantic water masses and currents produced by the Viking20 model driven by observed monthly winds, a comparison of transport variability of observed and modeled data will be presented at three locations: Deep flow at the exit of the Labrador Sea at 53°N; upper layer transports between New Jersey and Bermuda (OLEANDER section) and between the southern tip of Greenland and Portugal (OVIDE section). Is the model reproducing the observed long-term behavior of the different components in phase and amplitude? Do the results permit identification of the processes leading to these variations in transport variability? Finally, is it possible to extend the observed variability pattern over the observed time span (15 years) to the total time range of the model simulations (60 years)?
Morgan, J Kent; Luzio, Gary A; Ammar, El-Desouky; Hunter, Wayne B; Hall, David G; Shatters, Robert G
Stylet sheath formation is a common feature among phytophagous hemipterans. These sheaths are considered essential to promote a successful feeding event. Stylet sheath compositions are largely unknown and their mode of solidification remains to be elucidated. This report demonstrates the formation and solidification of in ?ere (in air) produced stylet sheaths by six hemipteran families: Diaphorina citri (Psyllidae, Asian citrus psyllid), Aphis nerii (Aphididae, oleander/milkweed aphid), Toxoptera citricida (Aphididae, brown citrus aphid), Aphis gossypii (Aphididae, cotton melon aphid), Bemisia tabaci biotype B (Aleyrodidae, whitefly), Homalodisca vitripennis (Cicadellidae, glassy-winged sharpshooter), Ferrisia virgata (Pseudococcidae, striped mealybug), and Protopulvinaria pyriformis (Coccidae, pyriform scale). Examination of in ?ere produced stylet sheaths by confocal and scanning electron microscopy shows a common morphology of an initial flange laid down on the surface of the membrane followed by continuous hollow core structures with sequentially stacked hardened bulbous droplets. Single and multi-branched sheaths were common, whereas mealybug and scale insects typically produced multi-branched sheaths. Micrographs of the in ?ere formed flanges indicate flange sealing upon stylet bundle extraction in D. citri and the aphids, while the B. tabaci whitefly and H. vitripennis glassy-winged sharpshooter flanges remain unsealed. Structural similarity of in ?ere sheaths are apparent in stylet sheaths formed in planta, in artificial diets, or in water. The use of 'Solvy', a dissolvable membrane, for intact stylet sheath isolation is reported. These observations illustrate for the first time this mode of stylet sheath synthesis adding to the understanding of stylet sheath formation in phytophagous hemipterans and providing tools for future use in structural and compositional analysis. PMID:23638086
LeVine, D. M.; Koblinsky, C.; Howden, S.; Goodberlet, M.
The salinity of the open ocean is important for understanding ocean circulation, for understanding energy exchange with the atmosphere and for improving models to predict weather and climate. Passive microwave sensors at L-band (1.4 GHz) operating from aircraft have demonstrated that salinity can be measured with sufficient accuracy (1 psu) to be scientifically meaningful in coastal waters. However, measuring salinity in the open ocean presents unresolved issues largely because of the much greater accuracy (approx. 0.1 psu) required to be scientifically viable. In the summer of 1999 a series of measurements called, The Gulf Stream Experiment, were conducted as part of research at the Goddard Space Flight Center to test the potential for passive microwave remote sensing of salinity in the open ocean. The measurements consisted of a compliment of airborne microwave instruments (radiometers and scatterometer) and ships and drifters for surface truth. The study area was a 200 km by 100 km rectangle about 250 km east of Delaware Bay between the continental shelf waters and north wall of the Gulf Stream. The primary passive instruments were the ESTAR radiometer (L-band, H-pol) and the SLFMR radiometer (L-band, V-pol). In addition, the compliment of instruments on the aircraft included a C-band radiometer (ACMR), an ocean wave scatterometer (ROWS) and an infrared radiometer. A GPS backscatter experiment was also part of the package. These instruments were mounted on the NASA P-3 Orion aircraft. Surface salinity measurements were provided by the RN Cape Henlopen and MN Oleander (thermosalinographs) plus salinity and temperature sensors on three surface drifters deployed from the RN Cape Henopen. The primary experiment period was August 26-September 2, 1999. During this period the salinity field within the study area consisted of a gradient on the order of 2-3 psu in the vicinity of the shelf break and a warm core ring with a gradient of 1-2 psu. Detailed maps were made with the airborne sensors on August 28 and 29 and on September 2 flights were made over the surface drifters to look for effects due to a change in surface roughness resulting from the passage of Hurricane Dennis. Preliminary results show a good agreement between the microwave measurements and ship measurements of salinity. The features of the brightness temperature maps correspond well with the features of the salinity field measured by the ship and drifters and a preliminary retrieval of salinity compares well with the ship data.
Ruamthum, W; Visetson, S; Milne, J R; Bullangpoti, V
The molluscicidal activity of crude extracts from five highly potential plants, Annona squamosa seed, Nerium indicum Leaves, Stemona tuberose root, Cyperus rotundus corm and Derris elliptica root was assessed to Pomacea canaliculata. D. elliptica root and C. rotundus corm extracts showed the highest toxicity against 3-month old snails which have LC50 as 23.68 +/- 2.96 mg/l and 133.20 +/- 7.94 mg/l, respectively. The C. rotundus corm extracts were chosen for detoxification enzyme in vivo assay which shows esterase and glutathione S-transferase activity in stomach, intestinal tracts and digestive glands of survival treated P. canaliculata were inhibited. PMID:21542482
Rao, I G; Singh, D K
The effects of sublethal treatments (20% and 60% of LC(50)/24 h) with plant-derived molluscicides on the reproduction of the giant African snail Achatina fulica were studied. Azadirachta indica oil, Cedrus deodara oil, Allium sativum bulb powder, and Nerium indicum bark powder singly and binary combinations on reproduction and survival of A. fulica were investigated. Repeated treatment occurred on day 0, day 15, and day 30. These plant-derived molluscicides significantly reduced fecundity, egg viability, and survival of A. fulica within 15 days. Discontinuation of the treatments after day 30 did not lead to a recovery trend in the next 30 days. Day 0 sublethal treatment of all the molluscicides caused a maximum reduction in protein, amino acid, DNA, RNA, and phospholipid levels and simultaneous increase in lipid peroxidation in the ovotestis of treated A. fulica. It is believed that sublethal exposure of these molluscicides on snail reproduction is a complex process, involving more than one factor in reducing the reproductive capacity of A. fulica. PMID:11031309
Dey, Priyankar; Dutta, Somit; Sarkar, Mousumi Poddar; Chaudhuri, Tapas Kumar
CCl4 is a potent environmental toxin which cause liver damage through free radical mediated inflammatory processes. In this study, hepatoprotective capacity of Nerium indicum leaf extract (NILE) was evaluated on CCl4 induced acute hepatotoxicity in murine model. Animals were divided into 5 groups and treated as following: control group (received only normal saline), CCl4 group (received only CCl4), silymarin group (received CCl4 and 100mg/kg silymarin), NILE low group (received CCl4 and 50mg/kg NILE) and NILE high group (received CCl4 and 200mg/kg NILE). After 10 consecutive days of treatment, the levels of hepatic biochemical markers, malondialdehyde (MDA) content, peroxidase and catalase activities were measured as well as histopathological study was performed. Furthermore, liver explant cultures were set up as following: control (no treatment), CCl4 group (contained 25?l/ml CCl4), silymarin group (contained 25?l/ml CCl4 and 100?g/ml silymarin), NILE low group (contained 25?l/ml CCl4 and 25?g/ml NILE) and NILE high group (contained 25?l/ml CCl4 and 100?g/ml NILE). Hepatic transaminases and phosphatases, tumor necrosis factor-? (TNF-?) expression, nitric oxide (NO) release and cell viability were studied on the explant cultures. Phytochemical fingerprinting of NILE was performed by gas chromatography-mass spectrometry (GC-MS). The results showed that the biochemical parameters were overexpressed due to CCl4 administration, which were significantly normalized by NILE treatment. The findings were further supported by histopathological evidences showing less hepatocellular necrosis, inflammation and fibrosis in NILE and silymarin treated groups, compared to CCl4 group. GC-MS analysis revealed presence of different bioactive phytochemicals with hepatoprotective and antioxidant properties. Therefore, the present study indicate that NILE possesses potent hepatoprotective capacity to ameliorate haloalkane xenobiotic induced injured liver in murine model. PMID:25871905
Chen, Liang-Wei; Wang, Yan-Qin; Wei, Li-Chun; Shi, Mei; Chan, Ying-Shing
Parkinson's disease (PD) is a common and debilitating degenerative disease resulting from massive degenerative loss of dopamine neurons, particularly in the substantia nigra. The most classic therapy for PD is levodopa administration, but the efficacy of levodopa treatment declines as the disease progresses. The neuroprotective strategies to rescue nigral dopamine neurons from progressive death are currently being explored, and among them, the Chinese herbs and herbal extracts have shown potential clinical benefit in attenuating the progression of PD in human beings. Growing studies have indicated that a range of Chinese herbs or herbal extracts such as green tea polyphenols or catechins, panax ginseng and ginsenoside, ginkgo biloba and EGb 761, polygonum, triptolide from tripterygium wilfordii hook, polysaccharides from the flowers of nerium indicum, oil from ganoderma lucidum spores, huperzine and stepholidine are able to attenuate degeneration of dopamine neurons and sympotoms caused by the neurotoxins 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine (6-OHDA) in vitro and in vivo conditions. In addition, accumulating data have suggested that Chinese herbs or herbal extracts may promote neuronal survival and neurite growth, and facilitate functional recovery of brain injures by invoking distinct mechanisms that are related to their neuroprotective roles as the antioxidants, dopamine transporter inhibitor, monoamine oxidase inhibitor, free radical scavengers, chelators of harmful metal ions, modulating cell survival genes and signaling, anti-apoptosis activity, and even improving brain blood circulation. New pharmaceutical strategies against PD will hopefully be discovered by understanding the various active entities and valuable combinations that contribute to the biological effects of Chinese herbs and herbal extracts. PMID:17691984