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1

Indici di performance neuromotoria valutati con piattaforme robotiche: osservazioni preliminari  

Microsoft Academic Search

Introduzione Lo stroke causa circa 5 milioni di vittime l'anno nel mondo, ed è la prima causa di impairment motorio. Gli approcci riabilitativi attual- mente conosciuti consistono nel recupero motorio e funzionale della disabilità motoria conseguente l'episodio ictale. Recenti ricerche in neuroscienze hanno mostrato come la plasticità neuronale stimolata dall'attività motoria è alla base del recupero neurale post-ictale; per questo

E. GALLOTTA; G. MAGRONE; L. M. LAPENNA; A. LISTRANI; L. ZOLLO; E. GUGLIELMELLI; S. STERZI

2009-01-01

2

Riduzione all’equatoke ed all’equinozio delle osservazioni di durate medie di colorazioni crepuscolari di nttbi (già ridotte al caso del sole puntiforme) onde renderle comparabili  

Microsoft Academic Search

Sunto  Si ritiene come da precedents lavori dell’A., ehe attorno al globo terracqueo esistano. certi spettri amtlari che colorano\\u000a le nubi, se queste li tagliano net rotare insieme alia terra. Ognuno di tali anelli, paragonato a sè stesso, deve essere owmque\\u000a di identica forma e spessore, ove si trascuri il lieve sehiacciamento terrestre (2) e si consideri la terra come sferica,

P. Barreca

1930-01-01

3

Osservazioni sul vacuolo contratlile di Colpoda cucullus  

Microsoft Academic Search

A research is made on the pulsation rate of the contractile vacuole in normal and treated clones of Colpoda cucullus. Both cultures treated that were put again back into the normal medium, one for twelve days, the other for thirteen days, show the same pulsation rate as the normal ones. The speed of the contractile vacuole formation seems to be

Tina Franceschi

1958-01-01

4

Osservazioni di Urano e Nettuno nel 2001  

NASA Astrophysics Data System (ADS)

The observations of Uranus and Neptune collected by the UAI Planet Section during the year 2001 are analysed. The Uranus disk in some occasion had presented a bright eccentric zone. The different colours of the border probably is an effect of atmospheric refraction. The Neptune disk was featureless. The methan band in the spectra of both planets was registered at different time but no changes were observed. Some indications for the next opposition of remote planets are given.

Carbognani, Albino

2002-09-01

5

Osservazioni Sull'attività Cambiale Di Nerium Oleander L  

Microsoft Academic Search

Observation on the cambial activity in «Nerium oleander» L. — The cambial activity of «Nerium oleander» has been observed in three localities in Southern Italy: Bari and Lucera, where the oleander is cultivated, and Policoro, in the bed of the river Sinni, where the oleander is spontaneuos. The wood is of the diffuse-porous type. Growth rings corresponding to distinct periods

Olimpia Bucci

1967-01-01

6

Osservazioni ecologiche su alcuni Antozoi del golfo di Genova  

Microsoft Academic Search

A small area of rocky shore (depth 10–40 m.) has been investigated near Portofino (Genua) and Anthozoa have been a particular object of research. With the help of divers, a good material is now gathered. Seven species of Madreporaria are present. Leptopsammia pruvoti and Madracis pharensis are chiefly interesting: the former as it is very abundant, the latter as was

Lucia Rossi

1956-01-01

7

Osservazioni Sulle Infrastrutture di Anabaena Cycadinae E delle Cellule dei Tubercoli Radicali di Cycas Revoluta  

Microsoft Academic Search

Observations on the infrastructures of ANABAENA CYCADINAE and of root-nodules cells of CYCAS REVOLUTA. — Light and electron microscope observations carried on the fine and ultrafine sections of root-nodules of Cycas revoluta, brought in evidence differences structural and infrastructural of cells. These differences concern mainly the modifications presented by reticulum endoplasmic which in middle-aged cells takes a fragmentary and vescicular

Maria Grilli

1963-01-01

8

Generazione di mesoni da parte di fotoni  

Microsoft Academic Search

Riassunto  In relazione alle esperienze di Berkeley sulla generazione di mesoni da parte di fotoni, si calcolano nella prima approssimazione\\u000a della teoria delle perturbazioni (eg) le sezioni d'urto differenziali per il processo in questione; si fa uso della teoria pseudoscalare con accoppiamento pseudoscalare\\u000a e della teoria scalare con accoppiamento scalare. Al contrario che in lavori precedenti, nel calcolo qui sviluppato si

G. Morpurgo

1950-01-01

9

Generazione di mesoni da parte di fotoni  

Microsoft Academic Search

\\u000a Riassunto  In relazione alle esperienze di Berkeley sulla generazione di mesoni da parte di fotoni, si calcolano nella prima approssimazione\\u000a delia teoria delie perturbazioni (eg) le sezioni d’urto differenziali per il processo in questione; si fa uso delia teoria pseudoscalare con accoppiamento pseudoscalare\\u000a e delia teoria scalare con accoppiamento scalare. Al contrario che in lavori precedenti, nel calcolo qui sviluppato si

G. Morpurgo

1950-01-01

10

ANALISI DI STABILITÀ DEL MODELLO DI UN AUTOVETTURA  

Microsoft Academic Search

Sommario Il lavoro è incentrato sull'analisi di biforcazione di un modello di vettura che sta affrontando una curva. Il modello ha 2 gradi di libertà, e le sue equazioni contengono le non linearità dovute alla curve caratteristiche degli pneumatici. Dopo aver analizzato l'andamento degli equilibri al variare di un parametro tramite metodi di continuazione, viene fatta l'analisi di biforcazione al

Giampiero Mastinu; Fabio Della Rossa; Vitaliano Nobile

11

[Standardization of di-chlor-di-para-xylilene, di-bromine-di-para-xylilene and di-para-xylilene].  

PubMed

Studied were the new components of dielectric coatings: di-para-xylilene, di-bromine-di-para-xylilene, dichlor-di-para-xylilene, which manifested themselves as low-toxicity compounds free from irritating, sensitizing and fibrogenous effects. The acute action thresholds for aerosols of the paraxylilenes were established at 10 mg/m3 (with regard to general and embryotoxicity), at 5 mg/m3 for the maximal inactive concentrations. PMID:1817083

Frolova, A D; Minkina, N A; Vasil'kovski?, V G; Kuznetsova, O A; Dolgopolova, E L; Shaposhnikova, E S; Martinson, T G

1991-01-01

12

ANALISI DEI PROFILI DI MISSIONE PER VEICOLI MILITARI. DEFINIZIONE E VALIDAZIONE DI STRUMENTI PER LA SINTESI DI CONDIZIONI DI CARICO EQUIVALENTI  

Microsoft Academic Search

Sommario Obiettivo della presente memoria è stata l'individuazione delle condizioni di carico da utilizzare per verificare il comportamento di veicoli militari in fase di progetto. Ciò significa individuare dei percorsi di prova (test tracks o condizioni di carico), le associate velocità di percorrenza ed il relativo chilometraggio al fine di definire una loro combinazione (test ring o combinazione di carico)

C. Braccesi; F. Cianetti; L. Silvioni

13

La formazione dell'interprete di comunità. Analisi linguistica comparata di interpretazioni di comunità \\  

Microsoft Academic Search

RIASSUNTO In uno scenario di globalizzazione, l'interprete di comunità diventa una figura sempre più necessaria. Altrettanto necessaria sembra l'individuazione delle capacità coinvolte nel community interpreting, nonché l'elaborazione di metodologie didattiche che promuovano lo sviluppo di tali capacità nello studente. Questo progetto si propone di rispondere, seppur in minima parte, a questa esigenza. Lo fa con l'ambizione di coprire due lingue

Natacha Niemants

2008-01-01

14

Sulla dinamica relativistica di un fluido ideale conduttore di calore  

Microsoft Academic Search

Sunto  Si stabiliscono le equazioni fondamentali della dinamica relativistica di un fluido ideale conduttore di calore, muovendo\\u000a dai principi della dinamica di un corpuscolo a massa di quiete variabile. Successivamente si deduce il tensore energetico\\u000a del suddetto mezzo che, solo nella forma, appare identico a quello dei fluidi ideali isentropici.

Giovanni Carini

1973-01-01

15

Assorbimento di saccarosio in ipocotili di ravanello (Raphanus sativus L.)  

Microsoft Academic Search

In questo lavoro abbiamo determinato le caratteristiche di entrata del saccarosio in funzione della sua concentrazione esterna, incubando i pezzi in saccarosio triziato, in condizioni normali e di stress osmotico. Nelle parti apicali l'assorbimento di saccarosio esogeno è lineare in presenza di basse concentrazioni mentre non lo è ad alte concentrazioni. Per quanto riguarda le parti basali la linearità si

Maurizio Sajeva; Francesca Grisafi; Anna Maria Ferrarella

1996-01-01

16

Tecniche di chirurgia radioguidata  

Microsoft Academic Search

\\u000a Le più avanzate tecniche diagnostiche di imaging pre-operatorio (TC, RM, ultrasuoni, SPECT, PET, PET\\/TC) forniscono al chirurgo\\u000a oncologo informazioni sempre più precise sulla localizzazione e sull’estensione della malattia neoplastica. Queste informazioni\\u000a permettono al chirurgo di ottimizzare il tipo di intervento, salvaguardandone la radicalità per quanto riguarda l’asportazione\\u000a del tumore, ma al tempo stesso limitandone l’invasività rispetto ai tessuti sani. Nonostante

Gianpiero Manca; Elisa Biggi; Fabiola Cocco; Rosanna Raschillà; Isabella Raugei

17

PER LA BIBLIOTECA DI LEONARDO: «LIBRO DI GIORGIO VALLA»  

Microsoft Academic Search

L'annotazione «Libro di Giorgio Valla» nell'elenco del Codice Madrid II di Leonardo da Vinci è probabilmente da riferire a Georgio Valla interprete, Nicephori Logica etc., stampato a Venezia presso Simone Bevilacqua nel 1498.

Claudio Scarpati

2000-01-01

18

Ricerca di sostanze psicotrope in campioni urinari di poliassuntori: confronto di metodiche immunochimiche e di spettrometria di massa (I e II livello)  

Microsoft Academic Search

L'abuso delle droghe di sintesi è sensibilmente aumentato in questi ultimi anni. Le ricadute del fenomeno ci hanno indotti all'analisi di due test immunochimici, comunemente utilizzati nella ricerca delle principali classi di sostanze psicotrope, confrontandone i risultati con quelli ottenuti da indagini in gascromatografia (GC) abbinata alla spettrometria di massa (MS). Per lo studio sono stati selezionati 33 campioni urinari

Denise Caneparo; Alberto Salomone; Elsa Basili; Marco Vincenti; Consorzio Piemontese

2005-01-01

19

Neuropsicologia delle dinamiche di acquisto  

Microsoft Academic Search

Il neonato dominio del neuromarketing è stato accostato in primis all’altrettanto giovane disciplina della neuroeconomia [1–3], come ambito di studio del processo di scelta in contesti economici e come versante applicativo delle dinamiche di acquisto\\u000a [4]. Occorre tuttavia precisare meglio quale sia la natura del rapporto tra le due discipline.

M. Balconi

20

Il problema della velocitá di combustione di un razzo ibrido  

Microsoft Academic Search

\\u000a Sunto  I motori razzo ibridi sono di notevole attualitá per i numerosi vantaggi che offrono, tra cui l’uso di metalli leggeri e la\\u000a possibilitá di «stop-star».\\u000a \\u000a Problema fondamentale dei razzi ibridi é la determinazione della velocitá di combustione che risulta indipendente dalla velocitá\\u000a di reazione.\\u000a \\u000a \\u000a \\u000a Essenzialmente ció che controlla il processo é il tempo necessario alle specie reagenti per unirsi. Quindi

Irvin Glassman

1967-01-01

21

Certain Di-Phthalonitriles.  

National Technical Information Service (NTIS)

An object of this invention is the di-phthalonitrile monomers, prepolymers and polymers which are devoid of ether and/or phenoxy groups. Another object of this invention is the high temperature polymers which can withstand temperatures in excess of 300 de...

T. M. Keller

1992-01-01

22

Teoria generale della radiazione di Cerenkov  

Microsoft Academic Search

Riassunto  Si istituisce la teoria della radiazione di Cerenkov emessa da una carica in moto vario. Lo sviltrppo di tale teoria è stato\\u000a reso possibile dall’introduzione di un nuovo metodo di determinazione del oampo elettromagnetico generato da una carica in\\u000a moto, metodo di portata. quanto mai generale. Il lavoro consta di una breve introduzione e di. tre capitoli. Nel primo capitolo

G. Zin

1961-01-01

23

AMBIENTE INTEGRATO DI PROGETTAZIONE ASSISTITA DI ROLLERCOASTER: ANALISI DEL COMPORTAMENTO DINAMICO E STRUTTURALE DELLA VETTURA  

Microsoft Academic Search

Sommario In questo lavoro viene illustrato uno strumento di modellazione e simulazione multicorpo di roller coaster che si inserisce in una più ampia procedura di progettazione sviluppata per conto di una delle maggiori aziende del settore. Il codice e la metodologia sviluppati investono l'ideazione, il disegno, la simulazione e la verifica funzionale e dinamica di questa particolare tipologia di giostre

C. Braccesi; F. Cianetti; G. Lori

24

DEFINIZIONE DI UNA PROVA DI ACCETTAZIONE SEMPLIFICATA ED ANALISI STATISTICA DEL COMPORTAMENTO VIBROACUSTICO DI RUOTE FERROVIARIE SMORZATE  

Microsoft Academic Search

Sommario Nel presente lavoro viene illustrata una procedura di accettazione di ruote ferroviarie trattate, nelle quali la ridotta emissione acustica è ottenuta mediante l'applicazione di uno smorzamento a strato vincolato mediante l'interposizione di un polimero viscoelastico fra il centro ruota ed un pannello di lamiera opportunamente sagomato. La procedura proposta è basata esclusivamente sull'analisi delle ampiezze di vibrazione in condizioni

A. Bracciali; S. Cervello; A. Rossi

25

ANALISI TEORICHE E PROVE SPERIMENTALI DI UN SISTEMA DI MONITORAGGIO DI FRANE CON EMISSIONE ACUSTICA  

Microsoft Academic Search

I sistemi di monitoraggio dei fenomeni franosi richiedono, nella fase preliminare, la conoscenza del tipo di movimento in atto o potenzialmente possibile nell'area esaminata, in modo da poter selezionare il sistema più efficace; è quindi fondamentale conoscere le caratteristiche geologiche del sito d'interesse. La seconda fase consiste nell'installazione degli strumenti adatti e nell'esecuzione della misure di monitoraggio: queste ultime possono

L. Sambuelli; M. Mondin; S. Franchi; C. Strobbia; G. Lollino

26

OSSERVAZIONI EMPIRICI NEL LAVORO DELLA DANZA SPORTIVA  

Microsoft Academic Search

The purpose of this research is to highlight how the dance sport (as well as in all sports in general) does not give much attention in the years prior in childhood. We have observed the work of three groups at the national level (dance schools) in central Italy for a week. The girls have an age between 13-15 years, very

Mario DI CARLO; Cezar HANTAU

2011-01-01

27

Matematica: passione giovanile di Stendhal  

Microsoft Academic Search

\\u000a Il 6 gennaio 1831 in margine a un libro che sta leggendo durante un viaggio Stendhal lascia un appunto su un’idea embrionale\\u000a di un’autobiografia che vorrebbe scrivere\\u000a \\u000a \\u000a Ho scritto le vite degli uomini più grandi, Mozart, Rossini, Michelangelo, Leonardo da Vinci. Adesso non ho più la pazienza\\u000a di cercare i materiali, di mettere a confronto le testimonianze, e mi è

Michele Emmer

28

L'epidemia di ADHD negli USA  

Microsoft Academic Search

Nel corso degli ultimi dieci anni, i casi di ADHD diagnosticati hanno raggiunto proporzioni epidemiche negli USA. Comportamenti che, un tempo, sarebbero stati considerati compresi nel campo di normalità, vengono oggi abitualmente definiti come patologici da chi ha interessi legittimi nella promozione dell'uso di psicofarmaci nella popolazione infantile ed adolescenziale. Il disturbo da deficit di attenzione con iperattività (ADHD) rappresenta

J. M. Stolzer

29

Studio sulla ricolonizzazione di un tratto di fiume Piave soggetto ad asciutte stagionali  

Microsoft Academic Search

RIASSUNTO La sperimentazione è stata svolta sul tratto medio del fiume Piave, in località Grave di Papadopoli, provincia di Treviso. In questo tratto il Piave scorre su uno spesso materasso ghiaioso e si divide in due rami, di Cimadolmo sulla sinistra e di Maserada sulla destra idrografica, periodicamente interessati da lunghi periodi di asciutta totale. Il presente lavoro si propone

Marco Zanetti; Diana Piccolo; Paolo Turin; Manuel Bellio; Roberto Venzo

30

Sulla relazione fra la teoria di Tomonaga-Schwinger e quella di Dirac-Fock-Podolsky  

Microsoft Academic Search

Riassunto  Si dimostra, mediante una opportuna estensione covariante di un metodo proposto daBecker eLeibfried, che, per particelle tutte di un solo tipo, la teoria di Dirac-Fock-Podolsky è equivalente a una formulazione della teoria\\u000a di Tomonaga-Schwinger relativa ad un numero finito di particelle.

P. Bocchieri; A. Loinger

1955-01-01

31

Sulla computazione di funzioni ricorsive  

Microsoft Academic Search

Sommario  In questo articolo introduciamo un Calcolo Funzionale per discutere il problema della computazione delle funzioui definite ricorsivamente.Come è ben noto dalla letteratura, la computazione di una funzione ricorsiva può dare differenti valori, a seconda che sia eseguita con la leftmost-outermost rule, o con la leftmost-innermost rule.Nel calcolo precedentemente menzionato diamo una condizione sufficiente perché la computazione di una funzione dipenda

G. De Michelis

1974-01-01

32

150 anni di crescita, 150 di divari: sviluppo, trasformazioni, politiche  

Microsoft Academic Search

This paper provides a first reading of the macroeconomic data and the main social indicators taken from the SVIMEZ volume 150 anni di statistiche italiane: Nord e Sud. 1861-2011, Il Mulino, 2011. Divided into three parts, the first one aims at rebuilding long-term trends in 150 years, in which the creation and persistence of the big question of territorial gap

Bianchi L; Miotti D; Padovani R; Pellegrini G; Provenzano G

2011-01-01

33

Iperglicemia postprandiale ed emoglobina glicosilata: nuovi fattori di rischio cardiovascolare  

Microsoft Academic Search

Nelle società industrializzate si sta registrando un grande aumento dei casi di diabete mellito di tipo 2 (DM-2), a causa dello stile di vita tipico di queste società (sedentarietà, alimentazio-ne squilibrata). Il DM è un ben noto fattore di rischio (FdR) di morbilità e morta- lità cardiova-scolare (CV), oltre che di mortalità totale; la caratteristica di tale patologia è l'iperglicemia

M. A. Pacilio

2005-01-01

34

La statistica di Bose e la teoria della radiazione di Dirac-Fermi  

Microsoft Academic Search

Sunto  Si dimostra sul fondamento della teoria della radiazione di Dirac Fermi che il numero delle complessioni distinte in un gas\\u000a fotonico è quello previsto dalla statistica di Bose ma che l’ammissione dell’equiprobabilità a priori di tale complessione\\u000a non è compatibile con la detta teoria della radiazione di Dirac Fermi.

Bruno Ferretti

1937-01-01

35

Produzione multipla di mesoni negli sciami penetranti  

Microsoft Academic Search

Riassunto  Si descrivono le esperienze riguardanti la produzione multipla dei mesoni nelle collisioni coi nuclei di alta energia eseguite\\u000a nel Laboratorio della «Testa Grigia» a 3 500 m. Il dispositivo sperimentale adottato (fig. 1) permette di registrare contemporaneamente\\u000a coincidenze del quarto, quinto e sesto ordine in due distinte apparecchiature perfettamente simmetriche, disponendo su una\\u000a di dette apparecchiature uno strato di paraffina

G. Bertolino; M. Cini; P. Colombino; G. Wataghin

1952-01-01

36

Toxicological Profile for Di-n-Octylphthalate.  

National Technical Information Service (NTIS)

Di-n-octylphthalate has been found in at least 300 of the sites on the NPL. Di-n-octylphthalate, also known as dioctyl phthalate, is a colorless, odorless, oily liquid. Di-n-octylphthalate is commonly used as a plasticizer (a substance added to plastics t...

P. M. Bittner J. B. Taylor

1997-01-01

37

Una rivista, lungo un quarto di secolo  

Microsoft Academic Search

Questo numero della Rivista di Storia Economica coincide con il compiersi del venticinquesimo anno della sua Seconda serie. La Prima serie, curata da Luigi Einaudi, era apparsa fra il 1936 e il 1943. Il numero si apre con un editoriale, dovuto a Pierluigi Ciocca e Gianni Toniolo, che riconsidera sinteticamente l'indirizzo di metodo e di contenuto seguito dalla Rivista nei

Ciocca Pierluigi; Toniolo Gianni

2009-01-01

38

CARATTERIZZAZIONE MECCANICA DI UN FILM PLASTICO BIODEGRADABILE E COMPOSTABILE  

Microsoft Academic Search

Sommario I principi di sostenibilità, ecoefficienza e chimica verde sono alla base di un nuovo modo di intendere le materie plastiche, che si traduce in una progettazione che interessa l'intero ciclo di vita del prodotto. Il lavoro presentato è stato incentrato sull'analisi di un nuovo materiale plastico, compostabile e biodegradabile al 100%, al fine di definirne le caratteristiche e verificarne

C. Casavola; A. Mastrandrea; C. Pappalettere

39

The "bestie di satana" murders.  

PubMed

In recent years, satanic groups have been responsible for various types and degrees of crimes. We report the case of a number of murders committed in Italy by a group of young people calling themselves the "Bestie di Satana". Forensic psychiatric assessment of the members of a satanic sect charged with the crime revealed that all the young people had a fragile, immature personality, a very low level of education and were socially disadvantaged. The trial of the members of the "Bestie di Satana" sect was concluded with the verdict of deliberate murder, and all the members were given long jail sentences. This report should lead us to explore social and cultural responses to juvenile satanism, statistically shown to be a relatively rare phenomenon but with a high criminal potential. PMID:24117906

Birkhoff, Jutta; Candelli, Chiara; Zeroli, Stefania; La Tegola, Donatella; Carabellese, Felice

2013-10-10

40

Smoke from DI Diesel Engines  

Microsoft Academic Search

\\u000a Although DI Diesel engines are becoming popular because of their fuel economy as well as low exhaust emissions, they emit\\u000a higher amount of visible exhaust termed as smoke. The characterization of this smoke has remained a challenge in engine development\\u000a and modelling work. A new phenomenological model is explained in this chapter encompasses the spray and the wall interaction\\u000a by

P. A. Lakshminarayanan; Yogesh V. Aghav

41

Perkembangan Pengukuran Kadester di Sarawak  

Microsoft Academic Search

Abstrak SemenJak tahun 1968. Sarawak telah menggunakan sistem koordlnat dalam unJuran Bentuk Benar Serong di Tepatl (BBS'I) dalarn sernua kerJa pengukuran pemetaan topografl dan kadester. Sebelum Itu. hanya kerJa pengukuran pernetaan topografi rnenggunakan koordinat dart unJuran BBST. sernentara ukur kadester rnenggunakan koordinat dart unJuran Casslnl. Perubahan sistern Inl dlharap dapat rneUclnkan kerJa pengukuran dan rnengelakkan kekeliruan terhadap nllal koordinat Dengan

Fakulti Ukur

42

ANALISI FEM DEL COMPORTAMENTO STRUTTURALE DI GIUNTI SALDATI IN TITANIO  

Microsoft Academic Search

Sommario La grande diffusione delle strutture saldate richiede una profonda conoscenza dei fattori che ne influenzano la resistenza a fatica soprattutto in condizioni di esercizio particolarmente gravose e sotto carichi variabili. Il cordone di saldatura rappresenta una vera e propria discontinuità geometrica che può essere sede di notevoli concentrazioni di tensione. Le prove di fatica condotte su giunti testa a

C. Casavola; L. Lamberti; C. Pappalettere; F. Tattoli

43

Efficacia di un training aerobico sulla percezione di fatica in donne mastectomizzate  

Microsoft Academic Search

Introduzione Negli ultimi anni si è assistito ad un miglioramento della prognosi della patologia neoplastica della mammella grazie allo sviluppo di efficaci programmi di screening che permettono una sempre più precoce diagnosi e grazie al continuo sviluppo in campo terapeutico e farmacologico. L'aumento della sopravvivenza si accompagna però alla presenza di effetti collaterali fisici e psicologici, dovuti alla neo- plasia

S. PISTILLO; B. FARABOLLINI; F. GRASSI; G. PASQUALINI; N. BATTELLI; L. ZEPPONI; L. PROVINCIALI; M. G. CERAVOLO

44

Simulazione della propagazione di difetti a fatica mediante il modello di zona coesiva  

Microsoft Academic Search

RIASSUNTO. Le giunzioni incollate guadagnano sempre più mercato, nel campo delle costruzioni in genere, dove è necessario un alleggerimento delle strutture. Nel caso di geometrie di giunto semplici il dimensionamento avviene attraverso relazioni analitiche che restituiscono il valore massimo delle tensioni, il quale deve essere inferiore al limite di utilizzo dell'adesivo stesso. Quando le geometrie sono complesse l'approccio analitico diventa

A. Pirondi; F. Moroni

45

Blushing effect of some carbohydrates on the green alga Dictyococcus cinnabarinus  

Microsoft Academic Search

Riassunto È studiato l'effetto di arrossamento provocato dall'aggiunta di alcuni zuccheri a colture sommerse dell'alga cloroficeaD. cinnabarinus. Questo effetto è dovuto alla formazione di cheto-carotenoidi, alla diminuzione delle clorofille ed alla degradazione dei cloroplasti. Le osservazioni al microscopio elettronico mettono in evidenza le variazioni della struttura dei cloroplasti.

F. Dentice di Accadia; Olga Gribanovski-Sassu; Nancy Lozano Reyes

1968-01-01

46

The DiGeorge anomaly.  

PubMed

The DiGeorge anomaly, DGA (formerly termed DiGeorge syndrome), is now known to be a developmental field defect in which pharyngeal pouch derivatives do not arise, usually because of inadequate neural crest contributions. The conditions in which this occurs include exposure to teratogens, cytogenetic abnormalities, and Mendelian disorders. As a result, the facies and cardiovascular defects which occur are very characteristic. Two rare conotruncal anomalies, type B interrupted aortic arch and truncus arteriosus account for over half of the cardiac lesions seen in DGA. Failure of descent of the thymus is extremely common in DGA, but immunodeficiency which requires correction occurs only in approximately 25% of the cases. The term, complete DGA, should be reserved for those patients in need of reconstitution of the immune system. One can identify those patients requiring treatment of the thymic defect by T cell enumeration and in vitro proliferation assays. Two alternatives for therapy are thymus transplantation and bone marrow transplantation from a HLA matched sibling. PMID:1931005

Hong, R

1991-01-01

47

OSSERVAZIONI SUL COMPORTAMENTO DI SUPERFICIE DEL GRANDE SQUALO BIANCO Carcharodon carcharias (L.) SU PREDE PASSIVE A DYER ISLAND (SUDAFRICA) OBSERVATIONS ABOUT SURFACE BEHAVIOUR OF THE GREAT WHITE SHARK Carcharodon carcharias (L.)IN PRESENCE OF PASSIVE PREYS AT DYER ISLAND (SOUTHAFRICA)  

Microsoft Academic Search

DURING 4 STUDY EXPEDITIONS IN SOUTHAFRICA, PERFORMED IN 2000, 2003, 2004 AND 2005, OBSERVATIONS ABOUT SURFACE PREDATORY BEHAVIOUR OF THE GREAT WHITE SHARK IN PRESENCE OF PASSIVE PREYS WERE MADE. OBSERVATIONS WERE CARRIED OUT BY UNLIMITED SHARK DIVING BOATS AND BY CAGE DIVING AROUND DYER ISLAND, ABOUT 5 MILES FAR FROM GANSBAAI. DURING THE 27 OBSERVATION DAYS WERE IDENTIFIED 78

P. MICARELLI; S. SPINETTI; S. TRIPEPI; E. SPERONE

48

Toxicological Profile for Di(2-ethylhexyl) phthalate.  

National Technical Information Service (NTIS)

The Agency for Toxic Substances and Disease Registry Toxicological Profile for Di(2-ethylhexyl) phthalate is intended to characterize the toxicological and health effects information for the substance. It identifies and reviews the key literature that des...

1989-01-01

49

Il sogno: un punto di vista neurologico  

Microsoft Academic Search

Nell’antichità i sogni erano spesso considerati di origine soprannaturale e si riteneva che, consentendo una comunicazione\\u000a diretta con gli dèi, potessero comunicare suggerimenti riguardo all’immediato futuro (sogni profetici e premonitori). Presso\\u000a gli Egizi, i Greci e i Romani gli interpreti dei sogni, che operavano in luoghi come quello dell’oracolo di Delfi, erano personaggi\\u000a apprezzati e potenti. Gli Egizi (il più

Claudio L. Bassetti; Matthias Bischof; Philipp Valko

50

Il surplus di produzione: conflitto e cambiamento  

Microsoft Academic Search

\\u000a Entrando in una qualunque agenzia di viaggi, troveremo poster che raccontano una storia economica molto interessante. Ci può\\u000a essere per esempio un poster dell’India con il Taj Mahal, e accanto una foto delle piramidi egizie, oppure un manifesto che\\u000a ci invita a visitare Roma, il Colosseo e i favolosi tesori di San Pietro o un poster della Francia, che pubblicizza

Samuel Bowles; Richard Edwards; Frank Roosevelt

51

Salute mentale ed alterazione delle abitudini di vita dei lavoratori giovani  

Microsoft Academic Search

RIASSUNTO. Le alterazioni delle abitudini di vita costituiscono fattori di rischio per la salute. Esse determinano da un lato un alto tasso di patologie e mortalità, e dall'altro la riduzione del rendimento lavorativo e l'aumento del rischio di infortuni, con ripercussioni rilevanti sia in termini di salute dei lavoratori che economici. Lo scopo di questo studio è di analizzare le

G. Tomei; C. Cangemi; R. Giubilati; M. Fioravanti

2007-01-01

52

Exclusive e+e-, di-photon and di-jet production at the Tevatron  

SciTech Connect

Results from studies on exclusive production of electron-position pair, di-photon, and dijet production at CDF in proton-antiproton collisions at the Fermilab Tevatron are presented. THe first observation and cross section measurements of exclusive e{sup +}e{sup -} and di-jet production in hadron-hadron collisions are emphasized.

Terashi, Koji; /Rockefeller U.

2007-05-01

53

Differential binding of 2'-biotinylated analogs of c-di-GMP with c-di-GMP riboswitches and binding proteins.  

PubMed

C-di-GMP has emerged as a signalling molecule that regulates a variety of processes in several bacteria; therefore there is interest in the development of biotinylated analogs for the identification of binding partners. No detailed study has been done to evaluate if biotinylated analogs of c-di-GMP are capable of binding to c-di-GMP receptors. Herein, we evaluate the binding of commercially available 2'-biotinylated c-di-GMP and phosphorothioate 2'-biotinylated c-di-GMP, prepared via a facile solid-phase synthesis, to several c-di-GMP receptors. Docking, using Autodock vina software, as well as experimental studies of these analogs, with c-di-GMP class I and II riboswitches and binding proteins, reveal that some, but not all, c-di-GMP receptors can tolerate the 2'-modification of c-di-GMP with biotin. PMID:22182995

Luo, Yiling; Zhou, Jie; Watt, Sarah K; Lee, Vincent T; Dayie, T Kwaku; Sintim, Herman O

2011-12-19

54

Cefalee emicraniche come fonte di ispirazione in un artista contemporaneo Migraine headaches as artistic inspiration in a contemporary artist  

Microsoft Academic Search

Un artista professionista di 34 anni con una storia di emicrania senza aura risalente almeno al periodo scolare, dall'età di 30 anni ha prodotto un gran numero di immagini di teste in fiamme e di teste spacca- te che ricordano le opere di arte emicranica dedicate ad illustrare il dolore provato durante un attacca di cefalea ed analoghe descrizioni rintracciabili

Hotel Dante; Klaus Podoll; Ubaldo Nicola

55

Incontrare la scienza a passo di danza: flamenco  

Microsoft Academic Search

\\u000a Arte e scienza, danza e fisica, flamenco e matematica... discipline diverse, prospettive diverse, lingue diverse. Dalla volontà\\u000a di coniugare queste diversità in una forma di comunicazione nuova e allettante nasce questo studio che ha trovato realizzazione\\u000a in una proposta scientifica del Museo Tridentino di Scienze Naturali di Trento. In un contesto divertente e stimolante, è\\u000a possibile avvicinarsi ai passi base

Samuela Caliari; Silvia Rensi

56

Problematiche anestesiologiche nei pazienti sottoposti a trapianto di organo solido  

Microsoft Academic Search

\\u000a Riassunto Il notevole miglioramento dei risultati e l’aumento della sopravvivenza ottenuti negli ultimi dieci anni nei trapianti\\u000a di organi solidi portano un sempre maggior numero di soggetti trapiantati a essere candidati a interventi chirurgici in elezione,\\u000a urgenza o emergenza. Complicanze legate a patologia vascolare arteriosa, patologie a carico di organi viscerali oppure l’insorgenza\\u000a di tumori de novo obbligano non solo

Andrea Gasperi; Andrea Corti; Aldo Cristalli; Manlio Prosperi; Ernestina Mazza

57

Diagnosi delle catene di maitra e teoria dei semigruppi  

Microsoft Academic Search

Introduzione-Sommario  Sotto la etichetta di «Diagnosi di circuiti digitali» sono in genere accomunati problemi di interesse e complessità differente. In ordine crescente di difficoltà, diagnosticare un circuito digitale può significare stabilire se il circnito in oggetto funziona come richiesto, oppure localizzare i punti dove è eventualmente guasto, oppure identificarne e la natura e la posizione dei guasti.Quando si faccia riferimento a

G. Fantauzzi

1970-01-01

58

Sopra la Teoria della Rimanenza e della Curva di Magnetizzazione  

Microsoft Academic Search

Sunto  \\u000a Scopo di questo lavoro è lo studio delle forze di risonanza, nel caso di un gran numero di atomi. La considerazione che il\\u000a cristallo si pud pensare distinto in zone elementari semplifica la trattazione del problema, perchè conduce a una F. H.in cui compaiono i momenti totali delle zone elementari.\\u000a \\u000a \\u000a Questo metodo è particolarmente adatto a studiare i fenomeni

G. Gentile

1934-01-01

59

CHANDRASEKHAR E IL LIMITE DI MASSA PER LE NANE BIANCHE  

Microsoft Academic Search

In questo contributo metterò in luce come Chandrasekhar sia arrivato a formulare il famoso limite di massa per le nane bianche che porta il suo nome, cercando di porre il lavoro dell'astrofisico indiano in relazione con le teorie della struttura stellare della sua epoca e concentrandomi prevalentemente sugli aspetti formali. Mostrerò come l'equazione di stato dei gas degeneri renda possibile

DAVIDE CENADELLI

60

Ricerche morfologiche ed istochimiche sulla glia del nevrasse di vertebrati  

Microsoft Academic Search

Con metodi istochimici e con metodi di colorazione e di impregnazione per il tessuto gliale fu studiato il materiale intercellulare interposto ai neuroni e fibre nervose nel nevrasse di anfibi urodeli ed anuri (Triton taeniatus, Triton cristatus, Amblystoma mexicanum, Xenopus laevis, Bufo vulgaris, Rana esculenta).

A. Bairati; G. Tripoli

1954-01-01

61

URINARY BIOMARKERS OF DI-ISONONYL PHTHALATE IN RATS  

EPA Science Inventory

Commercial di-isononyl phthalate (DiNP) is a mixture of various branched-chain dialkyl phthalates mainly containing ninecarbon alkyl isomers. At high doses in rodents, DiNP is a carcinogen, and a developmental toxicant. After exposure, the diester isomers are de-esterified to for...

62

Permasalahan Pelajar-pelajar Fakir Miskin di Daerah Sabak Bernam  

Microsoft Academic Search

ABSTRAK Kajian ini adalah satu tinjauan yang bertujuan untuk mengkaji permasalahan yang dihadapi oleh pelajar-pelajar dari golongan fakir miskin di negeri Selangor, khususnya di daerah Sabak Bernam. Seramai 42 orang pelajar Tingkatan 5 telah dipilih dalam kajian ini. Pelajar-pelajar ini terdiri daripada 19 pelajar yang tinggal di tiga buah Desa Kasih Pusat Zakat Selangor dan 23 orang pelajar yang tinggal

ZURIA MAHMUD; SAFANI BARI

63

PROFILI DI SOSTENIBILITÀ DELL'ADEGUAMENTO SISMICO DEGLI EDIFICI IN C.A  

Microsoft Academic Search

SOMMARIO Il comportamento dinamico di edifici in c.a. progettati per i soli carichi verticali è stato analizzato, al fine di valutarne la vulnerabilità sismica nonché l'efficacia di alcune tecniche di miglioramento, mediante analisi non lineari sia statiche che dinamiche su modelli di telai piani a plasticità concentrata, considerando diversi possibili comportamenti delle cerniere plastiche e utilizzando differenti gruppi di terremoti

Bruno Calderoni; Emilia Angela Cordasco; Pietro Lenza

64

L'obiettivo del progetto di legge alimentare è di migliorare la ...  

Center for Food Safety and Applied Nutrition (CFSAN)

... Margaret A. Hamburg, dice che il progetto di legge -- che il Presidente Barack Obama ha convertito in legge firmandolo il 4 gennaio -- getta le basi ... More results from www.fda.gov/food/guidanceregulation/fsma

65

Osteoporosi: la scienza di base, inquadramento, classificazione  

Microsoft Academic Search

L’osso è un tessuto connettivo specializzato, costituito da una matrice extracellulare mineralizzata e da una componente cellulare.\\u000a La matrice extracellulare del tessuto osseo è formata per il 40% da componenti organiche e per il 60% da componenti inorganiche.\\u000a La componente organica è rappresentata da collagene, proteoglicani, proteine non collageniche, fattori di crescita e citochine.

U. Tarantino; I. Cerocchi; A. Andreoli

66

Protein design: Engineering di-iron enzymes  

NASA Astrophysics Data System (ADS)

A dramatic switch of reactivity -- from hydroquinone oxidation to N-hydroxylation -- can be achieved through the rational engineering of a de novo-designed di-iron protein. Four specific amino-acid mutations spread throughout the first, second and third coordination shells result in a million-fold increase in the relative rate of these two reactions.

Berry, Steven M.

2012-11-01

67

Amylase activity of Torulopsis ingeniosa Di Menna  

Microsoft Academic Search

Torulopsis ingeniosaDi Menna was found to possess an ?-amylase strongly attached to the cell wall, its pH optimum being at 5.5, optimum temperature at\\u000a 50 °C, highly sensitive to thermal inactivation. The enzyme was found to be induced by starch but the synthesis is not subject\\u000a to a glucose effect.

G. Moulin; P. Galzy

1978-01-01

68

Livelli serici di insulina nel soggetto normale (Obeso e non) e nel diabetico obeso Dopo Carico Intragastrico di Aminoacidi  

Microsoft Academic Search

Riassunto Gli AA. hanno somministrato in 5 soggetti normali normosomici, in 5 obesi metabolicamente sani e in 5 diabetici obesi un carico intragastrico (mediante sondino) di 9 aminoacidi essenziali (arginina, fenil-alanina, valina, leucina, lisina, metionina, isoleucina, istidina, triptofano) per un totale di g 30 ed hanno valutato la risposta insulo-pancreatica. In tutti i casi di soggetti in esame si è

Ernesto Laudicina; Giandomenico Bompiani; Giacomo Angileri

1968-01-01

69

ANALISI TERMO-MECCANICA AGLI ELEMENTI FINITI DI UNA STRUTTURA INVESTITA DA FASCI DI PARTICELLE AD ALTA ENERGIA  

Microsoft Academic Search

Sommario Nel presente lavoro viene illustrata l'analisi termo-meccanica agli elementi finiti effettuata sui collimatori per il Large Hadron Collider, il potente acceleratore progettato al CERN di Ginevra; verranno esposte le scelte effettuate per i materiali e le soluzioni impiegate per il design dei collimatori di LHC. Verranno poi descritti il modello agli elementi finiti sviluppato, la metodologia di analisi adottata

A. Dallocchio; A. Bertarelli; L. Peroni

70

Biomaterial based sulphur di oxide gas sensor  

NASA Astrophysics Data System (ADS)

Biomaterials are getting importance in the present research field of sensors. In this present paper performance of biomaterial based gas sensor made of gum Arabica and garlic extract had been studied. Extract of garlic clove with multiple medicinal and chemical utility can be proved to be useful in sensing Sulphur di Oxide gas. On exposure to Sulphur di Oxide gas the material under observation suffers some temporary structural change, which can be observed in form of amplified potentiometric change through simple electronic circuitry. Exploiting this very property a potentiometric gas sensor of faster response and recovery time can be designed. In this work sensing property of the said material has been studied through DC conductance, FTIR spectrum etc.

Ghosh, P. K.; Sarkar, A.

2013-06-01

71

Di-organocobalt complexes of macrocyclic ligands  

Microsoft Academic Search

Three new di-metallorganic cobalt complexes of the type trans-(Bz)2Co(chel), where Bz is a benzyl group ?-bonded to cobalt atom and chel is an equatorial chelating system constituted by an amino-oximic ligand and its conjugated base, were synthesised. The protonated and the unprotonated ligands interact through an O–H?O bridge stabilising the entire structure. The complexes differ in the equatorial moiety which

Giovanni Tauzher; Renata Dreos; Alessandro Felluga; Nazario Marsich; Giorgio Nardin; Lucio Randaccio

2004-01-01

72

Di-boson physics at the Tevatron  

SciTech Connect

A summary is presented of recent measurements of di-boson production at the Tevatron. The results from the CDF and D0 experiments are based upon 130-320 pb{sup -1} of p{bar p} collisions at {radical}s = 1.96 TeV. The W{gamma}, Z{gamma}, WW, and WZ production properties are compared to Standard Model predictions, and limits extracted for anomalous triple gauge couplings.

Goshaw, A.T.; /Duke U.

2005-07-01

73

Di-hadron correlations at RHIC  

Microsoft Academic Search

Di-hadron correlations have been used to study jets at RHIC and have yielded rich insight into the properties of the medium. Studies show that the near-side peak of high-pT triggered correlations can be decomposed into two parts, a jet-like correlation and the ridge. The jet-like correlation is narrow in both azimuth and pseudorapidity and has properties consistent with vacuum fragmentation,

Christine Nattrass

2010-01-01

74

Business Cycle Based on Optimal DI Model  

NASA Astrophysics Data System (ADS)

We propose a dynamic model for the economic growth (? G) and the diffusion index (DI) in order to explain the business cycle. This model is described by equations analogous to the optimal velocity model in traffic flow. In the model there exists a conserved quantity, which corresponds to the total energy in a dynamical system. We found that the business cycle with the period 5--8 years is favorably reproduced, since the model predicts a periodic motion in the conservative system.

Taniguchi, M.; Bando, M.; Nakayama, A.

75

4-(Di-methyl-amino)-pyridinium trichlorido[4-(di-methyl-amino)-pyridine-?N]cobaltate(II)  

PubMed Central

In the anion of the title compound, (C7H11N2)[CoCl3(C7H10N2)], the CoII ion is coordinated by one N atom from a 4-(di­methyl­amino)­pyridine (DMAP) ligand and three Cl atoms, forming a CoNCl3 polyhedron with a distorted tetra­hedral geometry. In the crystal, cations and anions are linked via weak N—H?Cl and C—H?Cl hydrogen bonds. Double layers of complex anions stack along the b- axis direction, which alternate with double layers of 4-(di­methyl­amino)-pyridinium cations.

Guenifa, Fatiha; Hadjadj, Nasreddine; Zeghouan, Ouahida; Bendjeddou, Lamia; Merazig, Hocine

2013-01-01

76

Sullo spettro di energia degli elettroni negli sciami estesi di raggi cosmici  

Microsoft Academic Search

Riassunto  Viene studiato a Catania, 40 m s.l.d.m., mediante contatori di G.M., lo spettro di energia degli elettroni negli sciami estesi\\u000a dei raggi cosmici a due distanzed dal sistema che rivela gli sciami (d=0 ed=50 m) prendendo in considerazione successivamente sciami aventi nella parte centrale densità media ?=72; 42; 25; 12,5; 6,25;\\u000a 3,14 particelle\\/m2. I risultati sperimentali mostrano che lo spettro

C. Milone

1952-01-01

77

SULLA VALUTAZIONE NUMERICA DELLE CONDIZIONI ELASTOSTATICHE DI UN IMPIANTO DENTARIO ENDOSSEO  

Microsoft Academic Search

SOMMARIO Nel presente lavoro, affrontando dal punto di vista numerico le problematiche relative alla progettazione di un impianto dentario endosseo di forma e dimensioni tipo, sono stati ottenuti risultati relativi alla distribuzione tensionale e al campo degli spostamenti di modelli FEM e BEM parametrici, adattabili a varie forme di impianto in diverse condizioni implantologiche. L'approccio al lavoro, principalmente metodologico, tende

E. Armentani; F. Caputo; R. Citarella; R. Esposito; M. Infante; G. Lavorgna

78

MAPPATURA DELLE PIETRE PRESENTI NELLA FACCIATA DELLA CHIESA DI SAN FREDIANO (PISA, ITALIA)  

Microsoft Academic Search

Riassunto - In questo articolo si riporta la mappatura dei materiali utilizzati nella facciata della chiesa di San Frediano (Pisa, Italia), che è stata inserita, insieme ad altri monumen- ti ed opere d'arte di eccezionale importanza (Palazzo Vecchio a Firenze, Cattedrale di Toledo, opere di Masaccio e Masolino) come cantiere di ricerca diagnostica nell'ambito del Progetto Europeo «RIS+ Toscana: Trasferimento

M. LEZZERINI

79

Il problema di Malfatti nella letteratura matematica dell'800  

Microsoft Academic Search

Riassunto  In questa nota viene analizzato storicamente il problema legato al nome del matematico italiano Gianfrancesco Malfatti: «inscrivere\\u000a in un triangolo tre cerchi, ciascuno tangente agli altri due e a due lati del triangolo». Lo studio ha lo scopo di individuare\\u000a gli apporti più significativi dati a questo problema e di evidenziarne la posizione di centralità nella ricerca geometrica\\u000a dell'800.

Alessandra Fiocca

1980-01-01

80

PROGETTO A FATICA DI RUOTE DENTATE AD ELEVATE PRESTAZIONI  

Microsoft Academic Search

Sommario Il presente lavoro riguarda l'analisi sperimentale e la modellazione teorica delle prestazioni a fatica di ruote dentate per la trasmissione meccanica di auto da competizione, realizzate in acciaio ad elevata resistenza e sottoposte a cementazione, tempra, pallinatura e tribofinitura. Dopo una breve introduzione sull'attuale stato dell'arte dei metodi di progettazione a fatica, sono sintetizzati i principali risultati della ricerca

G. Olmi; M. Comandini; A. Freddi

81

Assistenza ventilatoria cronica nel paziente ospedalizzato* Quindici anni di esperienza  

Microsoft Academic Search

Obiettivi dello studio: I pazienti ventilati che non sono in grado di lasciare i reparti di terapia intensiva possono essere trasferiti ad un'unità per l'assistenza ventilatoria cronica (CAVC) con l'obiettivo di ottimizzare le loro capacità funzionali. In questo studio abbiamo riportato i risul- tati ottenuti in tutti i pazienti ricoverati in un'unità CAVC fra il 1986 ed il 2001. Pazienti

Peter J. Wijkstra; Monica A. Avendaño; Roger S. Goldstein

2003-01-01

82

Cyclic-di-GMP and cyclic-di-AMP activate the NLRP3 inflammasome.  

PubMed

The cyclic dinucleotides 3'-5'diadenylate (c-diAMP) and 3'-5' diguanylate (c-diGMP) are important bacterial second messengers that have recently been shown to stimulate the secretion of type I Interferons (IFN-Is) through the c-diGMP-binding protein MPYS/STING. Here, we show that physiologically relevant levels of cyclic dinucleotides also stimulate a robust secretion of IL-1? through the NLRP3 inflammasome. Intriguingly, this response is independent of MPYS/STING. Consistent with most NLRP3 inflammasome activators, the response to c-diGMP is dependent on the mobilization of potassium and calcium ions. However, in contrast to other NLRP3 inflammasome activators, this response is not associated with significant changes in mitochondrial potential or the generation of mitochondrial reactive oxygen species. Thus, cyclic dinucleotides activate the NLRP3 inflammasome through a unique pathway that could have evolved to detect pervasive bacterial pathogen-associated molecular patterns associated with intracellular infections. PMID:24008845

Abdul-Sater, Ali A; Tattoli, Ivan; Jin, Lei; Grajkowski, Andrzej; Levi, Assaf; Koller, Beverly H; Allen, Irving C; Beaucage, Serge L; Fitzgerald, Katherine A; Ting, Jenny P-Y; Cambier, John C; Girardin, Stephen E; Schindler, Christian

2013-09-06

83

Toluidine blue-O is a Nissl bright-field counterstain for lipophilic fluorescent tracers Di-ASP, DiI and DiO  

Microsoft Academic Search

The stain toluidine blue-O (tol blue), applied to sections of neural tissue, is shown to be compatible with the vivid fluorescent lipophilic neural tracers 4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide (Di-ASP), 3,3?-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI). As with other Nissl stains, toluidine blue-O fluoresces in the red end of the spectrum but such fluorescence quenches upon binding with tissue. Moreover, progressive staining

D. K Chelvanayagam; L. D Beazley

1997-01-01

84

Soluzioni algebriche dell’equazione di Lamé e torsione delle curve ellittiche  

Microsoft Academic Search

Sunto  Si discute il legame esistente tra equazioni di LaméL\\u000a n conn intero e soluzioni tutte algebriche, e punti di torsione di curve ellittiche. In particolare si esibisce una equazioneL\\u000a 1 con gruppo di monodromia proiettivo su\\u000a diedrale di ordine 6, legata ai punti di 3-divisione della curvay\\u000a 2=4x\\u000a 3?1.

F. Baldassarri

1987-01-01

85

L'errore nelle cure palliative secondo un'ottica costruttivista: presentazione di un caso clinico  

Microsoft Academic Search

Riassunto L'articolo nasce dall'esperienza personale di due operatori, una psicologa e un medico, che si occupano delle cure di fine vita. Nella nostra esperienza e confrontandoci con altri operatori, abbiamo più volte avvertito la difficoltà di conciliare la necessità di un approccio umanistico nella cura del malato con l'esigenza di rigore scientifico e metodologico. L'articolo tenta di dare una definizione

86

CIRI-CIRI KEJURUTERAAN TANAH BAKI GRANIT DI SEMENANJUNG MALAYSIA  

Microsoft Academic Search

Abstrak: Pengetahuan tentang kelakuan dan korelasi antara ciri-ciri tanah adalah penting dan berguna bagi rekabentuk dan ramalan pembinaan. Objektif utama kajian ini adalah untuk menentukan sifat-sifat kejuruteraan tanah baki granit di Semenanjung Malaysia. Tanah baki granit di Semenanjung Malaysia boleh dikelaskan sebagai tanah liat hingga pasir berkelodak dengan keplastikan rendah sehingga tinggi. Kandungan lembapan semulajadi bertambah dengan kedalaman dan kandungan

Aminaton Marto; Fauziah Kasim; Mohd Fairus Yusof

2004-01-01

87

Le strategie di riposizionamento delle local public utilities  

Microsoft Academic Search

Le strategie di riposizionamento delle local public utilities (di Barbara Antonioli, Roberto Fazioli, Alessandra Fazzioli) - ABSTRACt:In Italy, local public utilities are undergoing a radical change in organisational structure and strategically policy. The main aim is to diversify the core business in order to became multiutilities and multiservices firms. In this perspective, local public utilities are more and more interested

Alessandra Fazzioli; Roberto Fazioli; Barbara Antonioli

2002-01-01

88

Industrial Applications of HTS Coils Using DI-BSCCO Wire  

Microsoft Academic Search

Applications of the HTS coils are rapidly increasing since the Sumitomo's DI-BSCCO HTS wire has been developed and commercialized. This DI-BSCCO wire has almost the same mechanical properties as that of the copper wire while it can load a hundred times current. One of the applications of the HTS coils is the motor for the ship propulsion system. It reduces

T. Okazaki; K. Ohkura; T. Kato; K. Fujino; K. Hayashi; K. Sato

2006-01-01

89

Caratterizzazione di una camera a estrapolazione come campione di misura per radiazione beta e realizzazione di un complesso di irraggiamento per la taratura di dosimetri. (Characterization of extrapolation chamber as measurement scale for beta radiations and implementation of irradiation equipment for dosimeter calibration).  

National Technical Information Service (NTIS)

A computerized system for beta dosimetry has been developed at the Divisione di Metrologia e Protezione delle Radiazioni Ionizzanti dell'ENEA Italian Agency for (New Technologies, Energy and Environment)di Bologna. The system allows an absolute measuremen...

E. Fantuzzi F. Monteventi

1993-01-01

90

Agenti Cognitivi Semi-Intelligenti per Imprese Virtuali di e-Learning: Applicazioni di Metodologia TOGA e Tecnologie Web Multimediali  

Microsoft Academic Search

In questo lavoro è stata illustrata una analisi dei possibili ruoli e applicazioni dei agenti cognitivi semi-intelligenti (ACSI) inseriti nelle strutture concettuali dei sistemi di formazione e apprendimento a distanza. Gli agenti cognitivi sono stati definiti per i sistemi di e-Learning che utilizzano avanzate tecnologie multimediali e che possono essere accessibili tramite reti Internet, Intranet ed Extranet. La problematica è

Adam Maria Gadomski; Flavio Fontana

2003-01-01

91

catena-Poly[copper(II)-di-?-dicyan-amido-?-1,3-di-4-pyridylpropane].  

PubMed

In the title compound, [Cu(C(2)N(3))(2)(C(13)H(14)N(2))](n), the Cu(II) atom, located on an inversion centre, adopts a distorted octa-hedral coordination by six N atoms, two from 1,3-di-4-pyridylpropane and four from dicyanamide ligands, with significantly different Cu-N distances. The metal centres are linked in an unusual triple-bridged mode into chains parallel to [101]. PMID:21577406

Zhang, Jinfang

2009-08-08

92

catena-Poly[copper(II)-di-?-dicyan-amido-?-1,3-di-4-pyridylpropane  

PubMed Central

In the title compound, [Cu(C2N3)2(C13H14N2)]n, the CuII atom, located on an inversion centre, adopts a distorted octa­hedral coordination by six N atoms, two from 1,3-di-4-pyridylpropane and four from dicyanamide ligands, with significantly different Cu—N distances. The metal centres are linked in an unusual triple-bridged mode into chains parallel to [101].

Zhang, Jinfang

2009-01-01

93

Di-nitrogen fixation in a eutrophic tropical bay  

NASA Astrophysics Data System (ADS)

Di-nitrogen fixation, measured in situ by the acetylene reduction technique, was shown to be associated with regular summer blooms of Oscillatoria erythraea in Mazatlán bay, a eutrophic tropical embayment on the Pacific Coast of Mexico. The Oscillatoria population density and the rate of di-nitrogen fixation per cell were of similar magnitude to those observed by other workers in the oligotrophic Caribbean Sea. Since the non-nitrogen limited fraction ( Oscillatoria) of the total population was generally less than 10% and the nutrient supply to the bay is relatively abundant, di-nitrogen fixation does not appear to play an important role in the eutrophication of the bay.

Mee, Laurence D.; Cortes-Altamirano, Roberto; Garcia-de-la-Parra, Luz-Maria

1984-10-01

94

4,6-Di-bromo-N-{3-[(4,6-di-bromo-2,3-di-methyl-phenyl)imino]butan-2-yl-idene}-2,3-di-methyl-aniline  

PubMed Central

The title compound, C20H20Br4N2, is a product of the condensation reaction of 4,6-di­bromo-2,3-di­methyl­aniline and butane-2,3-dione. The mol­ecule has a center of symmetry at the mid-point of the central C—C bond. The dihedral angle between the benzene ring and the 1,4-di­aza­butadiene plane is 78.3?(2)°. Niether hydrogen bonding nor aromatic stacking is observed in the crystal structure.

Huang, Lina; Du, Zhengyin; Liu, Wei; Che, Fushou

2013-01-01

95

Modelling Service Requirements Variability: The DiVA Way  

NASA Astrophysics Data System (ADS)

This chapter tackles the challenges of variability identification, modelling and implementation for service-based systems. The DiVA methodology is applied to the Mobile Phone Service Portability case-study to demonstrate its solutions to these challenges. The DiVA methodology utilises concepts of Aspect-Oriented Software Development to encapsulate service variants in distinct modules and uses Model-Driven Development techniques to analyse and transform conceptual designs into executable services. The DiVA approach provides a tool-supported methodology for managing dynamic variability in adaptive systems and taming system complexity.

Greenwood, Phil; Chitchyan, Ruzanna; Ayed, Dhouha; Girard-Reydet, Vincent; Fleurey, Franck; Dehlen, Vegard; Solberg, Arnor

96

4,6-Di-bromo-N-{3-[(4,6-di-bromo-2,3-di-methyl-phenyl)imino]butan-2-yl-idene}-2,3-di-methyl-aniline.  

PubMed

The title compound, C20H20Br4N2, is a product of the condensation reaction of 4,6-di-bromo-2,3-di-methyl-aniline and butane-2,3-dione. The mol-ecule has a center of symmetry at the mid-point of the central C-C bond. The dihedral angle between the benzene ring and the 1,4-di-aza-butadiene plane is 78.3?(2)°. Niether hydrogen bonding nor aromatic stacking is observed in the crystal structure. PMID:24098193

Huang, Lina; Du, Zhengyin; Liu, Wei; Che, Fushou

2013-09-04

97

Labeling of Penicillamine di sulfide with technetium-99m  

Microsoft Academic Search

Complex forming conditions of Penicillamine di sulfide with 99mTc have been specified. Labeling of penicillamine di sulfide with 99mTc by direct reduction with SnCl2 did not give favorable good results while the 99mTc complex of penicillamine can be easily obtained. Ligand exchange reaction with 99mTc-gluconate was attempted and a 95% labeling efficiency was obtained. Radiopharmaceutical potential of 99mTc-PADS (99mTc-Penicillamine di

Perihan Ünak; Mehtap Tunç; Yusuf Duman

1998-01-01

98

Limb anomalies in DiGeorge and CHARGE syndromes  

SciTech Connect

Limb anomalies are not common in the DiGeorge or CHARGE syndromes. We describe limb anomalies in two children, one with DiGeorge and the other with CHARGE syndrome. Our first patient had a bifid left thumb, Tetralogy of Fallot, absent thymus, right facial palsy, and a reduced number of T-cells. A deletion of 22q11 was detected by fluorescence in situ hybridization (FISH). The second patient, with CHARGE syndrome, had asymmetric findings that included right fifth finger clinodactyly, camptodactyly, tibial hemimelia and dimpling, and severe club-foot. The expanded spectrum of the DiGeorge and CHARGE syndromes includes limb anomalies. 14 refs., 4 figs.

Prasad, C.; Quackenbush, E.J.; Whiteman, D.; Korf, B. [Harvard Medical School, Boston, MA (United States)

1997-01-20

99

ALERTINF: MODELLO DI PREVISIONE DELLE EMERGENZE PER IL CONTROLLO DELLE INFESTANTI DEL MAIS IN VENETO  

Microsoft Academic Search

La gestione delle infestanti è una pratica fondamentale nella coltivazione del mais. Il tempo di comparsa delle malerbe influenza la competizione tra infestante e specie coltivata determinando l'entità del danno che le malerbe possono provocare in termini di resa della coltura. La capacità di prevedere le emergenze delle malerbe può aiutare ad ottimizzare i tempi di controllo, può aumentare l'efficacia

Roberta Masin; Giuliano Cacciatori; Maria Clara Zuin; Giuseppe Zanin

100

Valutazione tecnico-economica delle potenzialità di riutilizzo irriguo dei reflui depurati: il caso della Valpadana  

Microsoft Academic Search

Gli effetti dei cambiamenti climatici (CC), sebbene soltanto vagamente ipotizzati qualche decennio fa, hanno cominciato a manifestarsi negli ultimi anni facendo emergere la vulnerabilità soprattutto del settore agricolo. Mentre le indicazioni politiche internazionali promuovono azioni congiunte di lungo periodo volte all’adozione di misure di mitigazione dei CC (riduzione dei gas serra), non sono ancora state chiaramente definite efficaci misure di

Raffaella Zucaro; C. Rago; Michele Vollaro

2012-01-01

101

SIMULAZIONE DI REAZIONI E PROCESSI IN MATERIALI PER L'HYDROGEN STORAGE  

Microsoft Academic Search

I materiali in stato solido per l'immagazzinamento di idrogeno sono i più promettenti per applicazioni auto- motive. Il miglioramento di questi materiali richiede una modellizzazione computazionale dei processi fisici e delle reazioni chimiche che avvengono durante la deidrogenazione. In questo articolo descriviamo i risultati ottenuti nello studio di questi processi sugli alanati di sodio, uno dei materiali più interessanti. •

Simone Meloni

102

Exposure and Risk Assessment for Phthalate Esters (Di(2-Ethylhexyl) Phthalate, Di-n-Butyl Phthalate, Dimethyl Phthalate, Diethyl Phthalate, Di-n-Octyl Phthalate, Butyl Benzyl Phthalate) (Revised).  

National Technical Information Service (NTIS)

This report assesses the risk of exposure to di(2-ethylhexyl) phthalate, di-n-butyl phthalate, dimethyl phthalate, diethyl phthalate, di-n-octyl phthalate, and butyl benzyl phthalate. This study is part of a program to identify the sources of and evaluate...

J. Perwak M. Goyer G. Schimke A. Eschenroeder J. Fiksel

1981-01-01

103

ParaDiS-FEM dislocation dynamics simulation code primer  

SciTech Connect

The ParaDiS code is developed to study bulk systems with periodic boundary conditions. When we try to perform discrete dislocation dynamics simulations for finite systems such as thin films or cylinders, the ParaDiS code must be extended. First, dislocations need to be contained inside the finite simulation box; Second, dislocations inside the finite box experience image stresses due to the free surfaces. We have developed in-house FEM subroutines to couple with the ParaDiS code to deal with free surface related issues in the dislocation dynamics simulations. This primer explains how the coupled code was developed, the main changes from the ParaDiS code, and the functions of the new FEM subroutines.

Tang, M; Hommes, G; Aubry, S; Arsenlis, A

2011-09-27

104

Cyclic di-AMP: another second messenger enters the fray.  

PubMed

Nucleotide signalling molecules contribute to the regulation of cellular pathways in all forms of life. In recent years, the discovery of new signalling molecules in bacteria and archaea, as well as the elucidation of the pathways they regulate, has brought insights into signalling mechanisms not only in bacterial and archaeal cells but also in eukaryotic host cells. Here, we provide an overview of the synthesis and regulation of cyclic di-AMP (c-di-AMP), one of the latest cyclic nucleotide second messengers to be discovered in bacteria. We also discuss the currently known receptor proteins and pathways that are directly or indirectly controlled by c-di-AMP, the domain structure of the enzymes involved in its production and degradation, and the recognition of c-di-AMP by the eukaryotic host. PMID:23812326

Corrigan, Rebecca M; Gründling, Angelika

2013-07-01

105

BISANZIO E LA CROCIATA: UNA LETTURA SECONDO L'ALESSIADE DI ANNA COMNENA  

Microsoft Academic Search

Nel periodo compreso tra la morte di Basilio II il Macedone (1025) e l'ascesa al trono di Alessio Comneno (1081), l'impero bizantino attraverso una delle fasi più travagliate della sua esistenza (1). Il Bulgaroctono era stato l'ultimo rappresentante di quella serie di imperatori energici che, utilizzando accortamente le forze disponibili, avevano saputo trovare al momento opportuno la maniera di risollevare

PASQUALE CORSI

106

Dermal oncogenicity bioassays of Di?sec?butoxyacetophenone and diethoxyacetophenone  

Microsoft Academic Search

Groups of 40 male C3H\\/HeJ mice were treated 3 times per week for their lifetime on the skin of the back with undiluted di?sec?butoxyacetophenone (DBAP), or with di?ethoxyacetophenone (DEAP), undiluted and as a 50% dilution in acetone. Approximate doses per application were 22.4 mg for DBAP, and 25.0 or 11.3 mg for DEAP. Two negative control groups received acetone only.

Linval R. DePass; Edward H. Fowler; Carrol S. Weil

1984-01-01

107

Photodegradation of polymer-dispersed perylene di-imide dyes  

Microsoft Academic Search

Polymer-dispersed perylene di-imide dye photodegradation is investigated by monitoring the fluorescence intensity as a function of 532 nm laser pulses. Anaerobically irradiated polymer-dye films exhibited an accelerated decrease in fluorescence intensity, which was partially recovered upon exposure to oxygen. Decelerated photodegradation rates were observed for perylene di-imide ethanol solutions upon the addition of a singlet oxygen quenching antioxidant. These observations

Nobuaki Tanaka; Nikolay Barashkov; Jerry Heath; Wade N. Sisk

2006-01-01

108

Upper limb malformations in DiGeorge syndrome  

SciTech Connect

We report on upper limb anomalies in two children with a complete DiGeorge sequence: conotruncal defects, hypocalcemia, thymic aplasia, and facial anomalies. One child had preaxial polydactyly, and the other had club hands with hypoplastic first metacarpal. In both patients, molecular analysis documented a 22q11 deletion. To our knowledge, limb anomalies have rarely been reported in DiGeorge syndrome, and they illustrate the variable clinical expression of chromosome 22q11 deletions. 13 refs., 2 figs.

Cormier-Daire, V.; Iserin, L.; Sidi, D. [and others

1995-03-13

109

Large HTS Magnet Made by Improved DI-BSCCO Tapes  

Microsoft Academic Search

Performance of the high temperature superconductor (HTS) is rapidly progressing and applications of the HTS are also improving. Significant enhancements in Jc in Bi2223 long wires have been achieved. The wires produced by Sumitomo Electric Ind., are named DI-BSCCO (Dynamically Innovative bismuth-based HTS wire). A cryo-cooled high-field high-temperature superconducting (HTS) magnet, which is made of this DI-BSCCO and has a

K. Ohkura; T. Okazaki; K. Sato

2008-01-01

110

Relazioni quantitative per lo stato tensionale di un qualunque sistema continuo e per la deformazione di un corpo elastico in equilibrio  

Microsoft Academic Search

Sunto.  Si stabiliscono delle disuguaglianze relative alle caratteristiche di tensione di un qualunque sistema continuo. Nel caso\\u000a dei corpi elastici omogenei communque anisotropi ne seguono disuguglianze relative alle deformazioni.

Giuseppe Grioli

1952-01-01

111

Interconfronti Dosimetrici per Fasci Esterni di Fotoni di Alta Energia. (Dosimetry Intercomparisons for High Energy Photon Beams).  

National Technical Information Service (NTIS)

In the framework of the Istituto Superiore di Sanita (ISS, Italian National Institute of Health) project 'Continuous Quality Improvement in Radiotherapy' a pilot project of dosimetry intercomparison among 16 Italian Radiotherapy Centres has been promoted....

S. Onori C. DeAngelis P. Fattibene

2002-01-01

112

Sono state realizzate tre serie di mappe con lo scopo di evidenziare isobate, isopache e distribuzione dei livelli porosi e permeabili dei tre Gruppi Acquiferi  

Microsoft Academic Search

Le mappe delle isobate evidenziano l'andamento nel sottosuolo della base di ogni singolo Gruppo Acquifero. Tali mappe sono il risultato dell'integrazione di 30.000 km di linee sismiche interpretate e dei dati di pozzo. Per prima cosa sono state riconosciute in sottosuolo, sulle sezioni sismiche, le superfici che individuano i principali corpi geologici e gli acquiferi in essi ospitati: i limiti

DELLE ISOBATA

113

Le cosí dette « orbite » di Bohr e di Bohr-Sommerfeld della quantistica classica e le onde associate agli elettroni nei varii livelli energetici  

Microsoft Academic Search

Sunto  Già l’A. aveva trovato, che a meno di un coefficiente di costipazione massima, « i raggi atomici sono multipli della lunghezza\\u000a d’ onda delle onde stazionarie di De Broglie associate all’elettrone in riposo ». Qui sono mostrate altre coincidenze, che\\u000a si riferiscono all’elettrone considerato, secondo la vecchia teoria dei quanta, in moto sulle cosiddette « orbite » stazionarie\\u000a di Bohr:

Mariaho Pierucci

1942-01-01

114

I marcatori biochimici di tumore associati alle tecniche per immagini medico-nucleari come esempi di integrazione diagnostica: i carcinomi della mammella, della prostata e dell'ovaio  

Microsoft Academic Search

L'iter diagnostico dei pazienti con neoplasia prevede l'impiego di diversi metodi tra cui molto importanti sono i test di laboratorio e le tecniche per immagini. L'interpretazione combinata dei test per i marcatori tumorali associati alle metodiche per immagini offre alcuni vantaggi che si traducono in una migliore accuratezza diagnostica. I marcatori tumorali forniscono una generica indicazione di presenza di malattia,

Carlo Villano; Flavio Crippa; Emilio Bombardieri

115

Biosynthesis of di-(2-ethylhexyl) phthalate (DEHP) and di- n-butyl phthalate (DBP) from red alga— Bangia atropurpurea  

Microsoft Academic Search

The contents of di-(2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) of red alga, Bangia atropurpurea, filaments cultured in artificial sea water medium were similar to those cultured in natural sea water medium. In the culture experiment, B. atropurpurea filaments were found to synthesize de novo phthalate esters. Additionally, DEHP and DBP contents in different species of algae grown in the

Chih Yu Chen

2004-01-01

116

Subchronic oral toxicity of di- n-octyl phthalate and di(2-ethylhexyl) phthalate in the rat  

Microsoft Academic Search

The subchronic oral toxicity of di(2-ethylhexyl) phthalate (DEHP) and di-n-octyl phthalate (DNOP) was studied. Groups of 10 male and 10 female Sprague-Dawley rats were administered DEHP in the diet at 0, 5, 50, 500 or 5000 ppm for 13 wk. In a separate study, groups of 10 male and 10 female Sprague-Dawley rats were given DNOP (5, 50, 500 and

R. Poon; P. Lecavalier; R. Mueller; V. E. Valli; B. G. Procter; I. Chu

1997-01-01

117

Chronic toxicity of di-n-butyl and di-n-octyl phthalate to Daphnia magna and the fathead minnow  

Microsoft Academic Search

The toxicities of di-n-butyl phthalate (DBP) and di-n-octyl phthalate (DOP) were assessed by measuring the effect of exposure to these compounds on the fecundity of Daphnia magna and on the hatching and survival of the early life stages of the fathead minnow Pimephales promelas. For D. magna, exposure to 1.8 mg\\/L DBP or 1.0 mg\\/L DOP caused a significant reduction

J. F. McCarthy; D. K. Whitmore

1985-01-01

118

Indeks Kualiti Air di Sekitar Kawasan Lombong di Pelepah Kanan, Kota Tinggi, Johor (Water Quality Index in the Vicinity of Pelepah Kanan Mine, Kota Tinggi, Johor)  

Microsoft Academic Search

ABSTRAK Kajian yang dijalankan ini bertujuan untuk menilai indeks kualiti air di sekitar kawasan lombong di Sungai Pelepah Kanan, Kota Tinggi, Johor. Sebanyak enam stesen pensampelan telah dipilih dari bahagian hulu ke hilir sungai ini untuk menilai indeks kualiti air di sepanjang sungai tersebut. Tiga replikasi sampel telah diambil daripada setiap stesen pensampelan. Pengambilan sampel dilakukan pada dua musim yang

WA N MOHD; RAZI IDRIS; SAHIBIN ABDUL RAHIM; MOHD TALIB LATIF; AZMAN HASHIM; SHAHRINIZAM MOHD YUSUF

119

Potent suppression of c-di-GMP synthesis via I-site allosteric inhibition of diguanylate cyclases with 2'-F-c-di-GMP.  

PubMed

Cyclic-di-GMP (c-di-GMP) is a central regulator of bacterial behavior. Various studies have implicated c-di-GMP in biofilm formation and virulence factor production in multitudes of bacteria. Hence it is expected that the disruption of c-di-GMP signaling could provide an effective means to disrupt biofilm and/or virulence factor formation in several bacteria of clinical relevance. C-di-GMP achieves the regulation of bacterial phenotype via binding to several effector molecules including transcription factors, enzymes and riboswitches. Crystal structure analyses of c-di-GMP effector molecules, in complex with the ligand, reveal that various classes of c-di-GMP receptors recognize this dinucleotide using different sets of recognition elements. Therefore, it is plausible that different analogues of c-di-GMP could be used to selectively modulate a specific class of c-di-GMP binding receptors, and hence modulate the bacterial phenotype. Thus far only a detailed study of the differential binding of c-di-GMP analogues to riboswitches, but not proteins, has been reported. In this report, we prepared various 2'-modified analogues of c-di-GMP and studied both polymorphisms of these analogues using DOSY NMR and the binding to several effector proteins, such as PilZ-containing proteins, diguanylate cyclases (DGC) containing I-sites, and phoshphodiesterases (PDE). 2'-Modification of c-di-GMP did not adversely affect the propensity to form higher aggregates, such as octameric forms, in the presence of potassium salts. Interestingly, we find that the selective binding to different classes of c-di-GMP binding proteins could be achieved with the 2'-modified analogues and that 2'-F analogue of c-di-GMP binds to the I-site of DGCs better (four times) than the native dinucleotide, c-di-GMP, whereas c-di-GMP binds to PDEs better (10 times) than 2'-F-c-di-GMP. 2'-F-c-di-GMP potently inhibits c-di-GMP synthesis by DGCs and hence raises the potential that cell permeable analogues of 2'-F-c-di-GMP could be used to disrupt c-di-GMP signaling in bacteria. PMID:23685177

Zhou, Jie; Watt, Sarah; Wang, Jingxin; Nakayama, Shizuka; Sayre, David A; Lam, Yiu-Fai; Lee, Vincent T; Sintim, Herman O

2013-04-29

120

Genotoxicity of di-butyl-phthalate and di-iso-butyl-phthalate in human lymphocytes and mucosal cells.  

PubMed

The genotoxicity of phthalates, widely used plasticizers, has been shown previously for di-butyl-phthalate (DBP) and di-iso-butyl-phthalate (DBP) in human mucosal cells of the upper aerodigestive tract in a previous study using the Comet assay. Furthermore, higher genotoxic sensitivities of patients with squamous cell carcinomas of either the larynx or the oropharynx compared to non-tumor patients were described. Other authors have demonstrated DNA damage by a different phthalate in human lymphocytes. It was the aim of the present study to determine whether there is a correlation between the genotoxic sensitivities to DBP and its isomer DiBP in either mucosal cells or lymphocytes. The single-cell microgel electrophoresis assay (Comet assay) was applied to detect DNA strand breaks in human epithelial cells of the upper aerodigestive tract (n=132 specimens). Human mucosa was harvested from the oropharynx in non-tumor patients and patients with squamous cell carcinomas of the oropharynx. Laryngeal mucosa of patients with laryngeal squamous cell carcinomas was harvested as well. Peripheral lymphocytes (n=49 specimens) were separated from peripheral blood. Xenobiotics investigated were DBP, DiBP, and N'methyl-N'-nitro-N-nitrosoguanidine (MNNG) as positive control, respectively. For statistical analysis, the SPSS correlation analysis according to Pearson and the Wilcoxon test were performed. Genotoxicity was found for DBP and DiBP in epithelial cells and lymphocytes (P<0.001). MNNG caused severe DNA damage. In analyzing DBP and DiBP results, genotoxic impacts in mucosal cells showed an intermediate correlation (r=0.570). Correlation in lymphocytes was the same (r=0.570). Phthalates have been investigated as a potential health hazard for a variety of reasons, including possible xenoestrogenic impact, peroxisome proliferation, and membrane destabilization. The present investigation suggests a correlated DNA-damaging impact of DBP and DiBP in human mucosal cells and in lymphocytes, respectively. PMID:11301413

Kleinsasser, N H; Wallner, B C; Kastenbauer, E R; Weissacher, H; Harréus, U A

2001-01-01

121

Synthesis, characterization, and applications of novel di-2-pyridyl imine ligands  

Microsoft Academic Search

Two novel di-2-pyridyl imines, 2,4,6-trimethyl-(di-2-pyridylmethylene)aniline (1) and 2,6-di-isopropyl-(di-2-pyridylmethylene)aniline (2), were prepared through condensation reactions between 2,2?-dipyridyl ketone and 2,4,6-trimethylaniline and 2,6-di-isopropylaniline. They reacted readily with bis(benzonitrile)dichloropalladium(II) to afford palladium imine complexes. The crystal structure of the palladium complex (3) bearing the 2,4,6-trimethyl-(di-2-pyridylmethylene)aniline ligand revealed coordination of a pyridyl group and an imine group to the metal center. The novel ligands

Jake Yorke; Catlin Dent; Andreas Decken; Aibing Xia

2010-01-01

122

Flavone-di- C-glycosides in citrus juices from Southern Italy  

Microsoft Academic Search

6,8-Di-C-glucopyranosylapigenin and 6,8-di-C-glucopyranosyldiosmetin were detected by HPLC–DAD–ESI–MS–MS in a variety of Southern Italian Citrus juices (orange, lemon, bergamot, citron, mandarin, clementine). In some juices, the amounts of C-glycosides detected were significant. 6,8-Di-C-glucopyranosylapigenin is characteristic of orange juice, while 6,8-di-C-glucopyranosyldiosmetin is the most important C-glycoside in lemon and citron juice. In bergamot juice the concentrations of 6,8-di-C-glucopyranosylapigenin and 6,8-di-C-glucopyranosyldiosmetin are similar.

Corrado Caristi; Ersilia Bellocco; Claudia Gargiulli; Giovanni Toscano; Ugo Leuzzi

2006-01-01

123

Penta-aqua-(di-methyl-formamide)-cobalt(II) sulfate di-methyl-formamide monosolvate.  

PubMed

The title compound, [Co(C3H7NO)(H2O)5]SO4·C3H7NO, contains five aqua ligands, a Co(II) atom, a sulfate ion and both a coordinating and a non-coordinating di-methyl-formamide (DMF) mol-ecule. The DMF solvent mol-ecule lies between the complex units, which are located along the b axis. The sulfate ion is for charge balance. The Co(II) atom has distorted octa-hedral coordination geometry, being ligated by five aqua ligands and the O atom of the DMF ligand. O-H?O hydrogen bonds between the aqua ligands and the sulfate anion and non-coordinating DMF molecule lead to the formation of a three-dimensional network. Since all constituents lie on a mirror plane, the H atoms of all methyl groups and of one of the aqua ligands are equally disordered over two positions. PMID:24046556

Ta?, Murat; Camur, Seval; Topal, Sevim

2013-06-08

124

Biological effects summary report di(2-ethylhexyl) sebacate  

SciTech Connect

An evaluation of the health effects of di(2-ethylhexyl) sebacate (DEHS) was made to assess its potential use as substitute for di(2-ethylhexyl) phthalate (DEHP), which is used as a test aerosol in quantitative fit testing of respirators. A review of published and unpublished information on toxicity of DEHS was made. The limited amount of data on DEHS indicated that it has a low level of toxicity to laboratory animals. No oral or inhalation toxicity studies on humans were found, but skin sensitization and irritation studies were negative. Insufficeint data exists at present to permit determination of DEHS carcinogenicity. Because of structural similarities of DEHS to DEHP and di(ethylhexyl) adipate, and because these latter two materials have been shown to be carcinogenic in rats and mice, DEHS is not recommended at this time as a DEHP substitute until lack of its carcinogenic potential is confirmed.

Silverstein, B.D.; White, O. Jr.; Brower, J.E.; Bernholc, N.M.

1983-10-01

125

Meson and Di-Electron Production with Hades  

NASA Astrophysics Data System (ADS)

The HADES experiment, installed at GSI, Darmstadt, measures di-electron production in A + A, p/? + N and p/? + A collisions. Here, the ?0 and ? Dalitz decays have been reconstruced in the exclusive p + p reaction at 2.2 GeV to form a reference cocktail for long-lived di-electron sources. In the C+C reaction at 1 and 2 GeV/u, these long-lived sources have been subtracted from the measured inclusive e+e- yield to exhibit the signal from the early phase of the collision. The results suggest that resonances play an important role in dense nuclear matter.

Fröhlich, I.; Agakishiev, G.; Agodi, C.; Balanda, A.; Bellia, G.; Belver, D.; Belyaev, A.; Blanco, A.; Böhmer, M.; Boyard, J. L.; Braun-Munzinger, P.; Cabanelas, P.; Castro, E.; Chernenko, S.; Christ, T.; Destefanis, M.; Díaz, J.; Dohrmann, F.; Dybczak, A.; Eberl, T.; Fabbietti, L.; Fateev, O.; Finocchiaro, P.; Fonte, P.; Friese, J.; Galatyuk, T.; Garzón, J. A.; Gernhäuser, R.; Gil, A.; Gilardi, C.; Golubeva, M.; González-Díaz, D.; Grosse, E.; Guber, F.; Heilmann, M.; Hennino, T.; Holzmann, R.; Ierusalimov, A.; Iori, I.; Ivashkin, A.; Jurkovic, M.; Kämpfer, B.; Kanaki, K.; Karavicheva, T.; Kirschner, D.; Koenig, I.; Koenig, W.; Kolb, B. W.; Kotte, R.; Kozuch, A.; Krása, A.; Krizek, F.; Krücken, R.; Kühn, W.; Kugler, A.; Kurepin, A.; Lamas-Valverde, J.; Lang, S.; Lange, J. S.; Lapidus, K.; Lopes, L.; Maier, L.; Mangiarotti, A.; Marín, J.; Markert, J.; Metag, V.; Michalska, B.; Michel, J.; Mishra, D.; Morinière, E.; Mousa, J.; Müntz, C.; Naumann, L.; Novotny, R.; Otwinowski, J.; Pachmayer, Y. C.; Palka, M.; Parpottas, Y.; Pechenov, V.; Pechenova, O.; Cavalcanti, T. Pérez; Pietraszko, J.; Przygoda, W.; Ramstein, B.; Reshetin, A.; Roy-Stephan, M.; Rustamov, A.; Sadovsky, A.; Sailer, B.; Salabura, P.; Schmah, A.; Simon, R.; Sobolev, Yu. G.; Spataro, S.; Spruck, B.; Ströbele, H.; Stroth, J.; Sturm, C.; Sudol, M.; Tarantola, A.; Teilab, K.; Tlusty, P.; Traxler, M.; Trebacz, R.; Tsertos, H.; Veretenkin, I.; Wagner, V.; Wen, H.; Wisniowski, M.; Wojcik, T.; Wüstenfeld, J.; Yurevich, S.; Zanevsky, Y.; Zhou, P.; Zumbruch, P.

126

SolidPhase Microextraction Coupled with High Performance Liquid Chromatography–UV Detection for the Determination of Di-n-Propyl-phthalate, Di-iso-Butyl-phthalate, and Di-Cyclohexyl-phthalate in Environmental Water Samples  

Microsoft Academic Search

A simple method for the determination of di-n-propyl-phthalate, di-iso-butyl-phthalate, and di-cyclohexyl-phthalate in water samples using solid-phase microextraction coupled to high performance liquid chromatography and UV detection has been developed. Several kinds of commercially available coating fibers were evaluated for their extraction performance to the target analytes and the CW\\/TPR coating fibre was found to be the best one. The parameters

Yaqi Cai; Guibin Jiang; Jingfu Liu

2003-01-01

127

Synthesis and photochemical properties of 1,3-di-9-anthryl-2-propanol, 1,3-di-9-anthrylacetone, and related propano-linked anthracenes  

SciTech Connect

9-anthryllithium reacts with epichlorohydrin to give 1,3-di-9-anthryl-2-propanol via 3-(9-anthryl)-1-chloro-2-hydroxypropane and 3-(9-anthryl)-1,2-epoxypropane. Oxidation of dianthryl-2-propanol with Dess-Martin reagent gave 1,3-di-9-anthrylacetone. Various other 1,3-bichromophoric anthracenes were synthesized by way of C-methylation of dianthrylacetone, 1,3-di-9-anthryl-1-propanone, and 1,3-di-9-anthrylpropenone. 9-Anthrylacetone, formed as byproduct in the reaction of anthryllithium with epichlorohydrin, was prepared from 9-anthrylacetaldehyde by Grignard reaction with methylmagnesium iodide and subsequent oxidation. The excited-state properties of dianthryl-2-propanol, dianthrylacetone, 1,3-di-9-anthryl-2-methyl-1-propanone, 1,3-di-9-anthryl-2-methyl-2-propanol, diastereomeric 1,3-di-9-anthryl-1-butanols, 1,3-di-9-anthryl-2-butanone, 1,3-di-9-anthryl-1-butanone, and diastereomeric 1,3-di-9-anthryl-2-methyl-1-butanones have been evaluated by fluorescence quantum yields, and by quantum yields for the isomerization by intramolecular 4{pi} + 4{pi} cycloaddition. Dianthryl-2-propanol, 1,3-di-9-antrhyl-2-methyl-2-propanol, dianthrylacetone, diastereomeric 1,3-di-9-anthryl-1-butanols, and 1,3-di-9-anthryl-2-butanone isomerize in their excited singlet state with quantum yields of 0.046, 0.021, 0.25, 0.23, and 0.22, respectively. The photochemical isomerization of those 1,3-bichromophoric anthrancenes which are characterized by a 9-anthroyl chromophore involves the excited triplet state, and the cyclization quantum yield may be as high as 0.72.

Becker, H.D.; Amin, K.A. (Chalmers Univ. of Technology, Gothenburg (Sweden))

1989-06-23

128

Metodo del Flusso Inverso Nella Teoria di Seconda Approssimazione in Aerodinamica Supersonica  

Microsoft Academic Search

\\u000a Sunto  In questa Nota viene ricavata una relazione generale tra il flusso inverso e il flusso diretto, nel quadro della teoria di\\u000a seconda approssimazione (nel parametro geometrico tipico di una superficie-ostacolo) per il flusso supersonico. Per mezzo\\u000a di essa, si dimostra che la variazione in seconda approssimazione della resistenza di un corpo quasi cilindrico arbitrario\\u000a può essere espressa con integrali estesi

Joseph H. Clarke

1964-01-01

129

Riboswitches in Eubacteria Sense the Second Messenger Cyclic Di-GMP  

Microsoft Academic Search

Cyclic di-guanosine monophosphate (di-GMP) is a circular RNA dinucleotide that functions as a second messenger in diverse species of bacteria to trigger wide-ranging physiological changes, including cell differentiation, conversion between motile and biofilm lifestyles, and virulence gene expression. However, the mechanisms by which cyclic di-GMP regulates gene expression have remained a mystery. We found that cyclic di-GMP in many bacterial

N. Sudarsan; E. R. Lee; Z. Weinberg; R. H. Moy; J. N. Kim; K. H. Link; R. R. Breaker

2008-01-01

130

L’uomo che visse con un buco in testa: la storia di Phineas Gage  

Microsoft Academic Search

\\u000a Phineas Gage era caposquadra di un’impresa edile, che lavorava nel 1840 alla costruzione di una nuova linea ferroviaria che\\u000a avrebbe attraversato il Vermont. Sovrintendeva ai lavori di scavo della roccia che bisognava far saltare in aria per spianare\\u000a il terreno e posare i binari. Un giorno commise un errore cruciale e un metro di barra metallica, usata per mandar giù

Geoff Rolls

131

L'ULTIMO INTERVENTO DI FILIPPO II IN TESSAGLIA NELLA PROPAGANDA MACEDONE E ANTIMACEDONE  

Microsoft Academic Search

L'intervento di Filippo II di Macedonia in Tessaglia contro gli Alevadi di Larissa è presentato come spedizione antitirannica dalla propaganda macedone riflessa in Isocrate, Speusippo, Diodoro, e in uno scolio a Demostene, mentre è connotato come l'azione violenta del re macedone contro gli ex alleati dalla propaganda antimacedone alimentata ad Atene e in Grecia soprattutto da Demostene. Le due contrastanti

Giuseppe Squillace

2000-01-01

132

Il Nuovo Sistema Informativo Sanitario (Nsis): modello concettuale di base Dicembre 2005  

Microsoft Academic Search

Il Servizio Sanitario Nazionale si colloca in uno scenario politico e sociale in profonda evoluzione, per effetto del progressivo decentramento di poteri e competenze dallo Stato alle Regioni e della necessità di garantire un maggiore equilibrio tra le risorse da destinare all'acuzie e alla cronicità. In questo contesto è emersa la necessità di costruire una base dati nazionale, condivisa con

Daniela Canali; Marta Fantola CNR-Istituto; Tecnologie Biomediche

133

Structure Reactivity Studies on the Extraction of Rare Earths by Di(2-ethylhexyl)chloroalkyl Phosphonates.  

National Technical Information Service (NTIS)

The extractive separation properties and the extractive regularity of the lanthanide elements by five di(2-ethylhexyl)alkyl phosphonates with various alkyl structure: di(2-ethylhexyl)chloromethyl phosphonate CH2ClP(O)(OC8H17-i)2 (1), di(2-ethylhexyl)beta-...

W. Z. Ye J. Y. Yan

1995-01-01

134

ESPRESSIONE DELLE PROTEASI NEGLI STADI LARVALI IV DI OSMIA CORNUTA EVIDENZIATA MEDIANTE ZIMOGRAFIA PROTEINASES PATTERN CHANGES IN OSMIA CORNUTA IV INSTAR LARVAE INVESTIGATED BY ZYMOGRAPHY  

Microsoft Academic Search

RIASSUNTO L'allevamento in pieno campo di Osmia cornuta è ancora necessario a causa della man- canza di informazioni sui meccanismi molecolari che regolano la nutrizione di tali apoidei. Lo scopo di questo studio è stato quello di dare un contributo nella descrizione della varia- zione del pattern proteasico così da dirottare, in parte, le strategie di allevamento in pieno campo

ELENA DONADIO; ETTORE BALESTRERI; MAURO PINZAUTI; ANTONIO FELICIOLI

135

Comparison of the short-term effects of di(2-ethylhexyl) phthalate, di(n-hexyl) phthalate, and di(n-octyl) phthalate in rats.  

PubMed

This study compares changes in the livers of rats treated with di(2-ethylhexyl) phthalate (DEHP) and its straight-chain analogs di(n-hexyl) phthalate (DnHP) and di(n-octyl phthalate (DnOP). Groups of rats were fed diets containing 20,000 ppm of one of these compounds. Subgroups were killed after 3, 10, and 21 days, and the livers were examined by histological, cytological, and biochemical methods. The results show considerable differences between the effects of the branched-chain phthalate ester DEHP and its straight-chain analogs. The major effects on the liver following administration of diets containing DEHP were midzonal and periportal accumulation of small droplets of lipid, hepatomegaly accompanied by an initial burst of mitosis, proliferation of hepatic peroxisomes and of smooth endoplasmic reticulum accompanied by induction of peroxisomal fatty acid oxidation, damage to the peroxisomal membranes as evidenced by increased leakage of catalase to the cytosol, and centrilobular loss of glycogen and falls in glucose-6-phosphatase activity and in low-molecular-weight reducing agents. In contrast, diets containing DnHP or DnOP induced accumulation of large droplets of fat around central veins leading, by 10 days, to mild centrilobular necrosis and a very slight induction of one peroxisomal enzyme and an increase in liver weight, but no significant changes in any other parameters which were affected by DEHP. PMID:3966235

Mann, A H; Price, S C; Mitchell, F E; Grasso, P; Hinton, R H; Bridges, J W

1985-01-01

136

Federico di Montefeltro's hyperkyphosis: a visual-historical case report  

Microsoft Academic Search

INTRODUCTION: The literature contains several publications describing the use of visual arts to develop observational skills in medical students. Portraits of individuals of the Italian Renaissance can be used to enhance these skills and stimulate the development of differential diagnoses in medical students. The Duke of Urbino, Federico di Montefeltro (1422–1482), lost his right eye and nasal bridge during a

Anthony V D'Antoni; Stephanie L Terzulli

2008-01-01

137

Storia e identita di un ente di ricerca. L'Istituto Superiore di Sanita attraverso racconti e testimonianze orali. (History and Identity of a Research Institute. The Istituto Superiore di Sanita Through Oral Stories and Memories).  

National Technical Information Service (NTIS)

The idea of this volume was born from the awareness of the importance to preserve and valorize the memories of those people who contributed to the history of the Istituto Superiore di Sanita, the main research institution for the safeguard of public healt...

D. Marsili P. D. Castro S. Modigliani

2011-01-01

138

Failures of rear axle shafts of 575 DI tractors  

Microsoft Academic Search

Rear axle shafts of 575 DI tractors manufactured by Mahindra and Mahindra Ltd, Tractor Division, Mumbai, failed before completion of warranty period. Most of the shaft failures (nearly 80–85%) occur during puddeling operations. Rear axle shafts fail in the spline portion. Cracks were found at the root of the splines. The causes of failure and remedies have been discussed in

G. K. Nanaware; M. J. Pable

2003-01-01

139

Individual diVerences in chess expertise: A psychometric investigation  

Microsoft Academic Search

Starting from controversies over the role of general individual characteristics (especially intelli- gence) for the attainment of expert performance levels, a comprehensive psychometric investigation of individual diVerences in chess expertise is presented. A sample of 90 adult tournament chess play- ers of varying playing strengths (1311-2387 ELO) was screened with tests on intelligence and person- ality variables; in addition, experience

Roland H. Grabner; Elsbeth Stern; Aljoscha C. Neubauer

2006-01-01

140

"DI Days:" Differentiating Instruction through Student Choice. Classroom Tips  

ERIC Educational Resources Information Center

|To better meet the needs of the author's high school students, who have a variety of abilities, interests and engagement levels, he often differentiates instruction through the use of "DI Days." On these days, students use a choice board to select from a wide range of activities offered in learning centers. Each center offers content-focused…

Hammond, Donald J.

2011-01-01

141

Performance Testing of the DiPerna Sweeper.  

National Technical Information Service (NTIS)

The DiPerna Sweeper, a partial-vacuum oil skimmer, was tested in a two-week test program conducted at the U.S. Environmental Test Tank (OHMSETT) in Leonardo, New Jersey. Forty-three oil recovery tests were run. The object of the program was to establish a...

M. K. Breslin

1981-01-01

142

Antioxidant properties of di- tert-butylhydroxylated flavonoids  

Microsoft Academic Search

Epidemiological evidence suggests an inverse relationship between dietary intake of flavonoids and cardiovascular risk. The biological activities of flavonoids are related to their antioxidative effects, but they also can be mutagenic, due to the prooxidant activity of the catechol pattern. To prevent these problems, we synthesized new flavonoids where one or two di-tert-butylhydroxyphenyl (DBHP) groups replaced catechol moiety at position

Jonathan Lebeau; Christophe Furman; Jean-Luc Bernier; Patrick Duriez; Elisabeth Teissier; Nicole Cotelle

2000-01-01

143

[Di George syndrome: not always a pediatric diagnosis].  

PubMed

We describe a delayed diagnosis of Di George syndrome, in a 51 yr-old woman, with past medical history of epilepsy, mental retardation, chronic psychosis, nephrocalcinosis. She presented facial dysmorphism, multiple encephalic calcifications, hypocalcemia and lymphopenia. A microdeletion of 22q 11.2 was detected by fluorescence in situ hybridization (FISH), confirming the clinical suspicion . PMID:23535960

Bertola, Giorgio; Giambona, Salvatore; Bianchi, Roberto; Girola, Andrea; Berra, Sergio Antonio

2013-02-01

144

DiVerential integration of kinaesthetic signals to postural control  

Microsoft Academic Search

The purpose of the present experiment was to identify whether non-visual sensory cues involved in the maintenance of balance control could be weighted diVerently from one subject to another in condition during which kinaesthetic signals, stemming from the ankle proprioceptors and plantar pressure somatosensory sensors, were altered. A large popula- tion of blindfolded healthy young university students (n = 140)

Brice Isableu; Nicolas Vuillerme

145

Biodegradation of di-(2-ethylhexyl) phthalate in sewage sludge  

Microsoft Academic Search

Di-(2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer in the production of polyvinyl chloride to impart flexibility to the product. Because of its mutagenicity and carcinogenicity, the presence of DEHP in sludge limits the application of sludge as a soil fertilizer. In this study, sludges were collected from three sewage treatment plants and thirteen wastewater treatment plants of different

H. F. Cheng; S. Y. Chen; J. G. Lin

146

Reply to Di Nucci: why the counterexamples succeed  

Microsoft Academic Search

In my essay, a critique of “the best secular argument against abortion” I reconstructed and criticised two versions of Don Marquis’s well-known argument against abortion. In critiquing the version I call the “essence argument”, I presented counterexamples to one of the premises in that argument. In this issue of the journal, Ezio Di Nucci takes note of the fact that

C Strong

2009-01-01

147

TOXICOLOGY OF MONO- AND DI-ALKYLTIN CHLORIDES.  

EPA Science Inventory

Mono- and di-alkyltin chlorides are reactive compounds used in the production of stabilizers for polyvinyl chloride (PVC) plastics, primarily used for water distribution pipes. Health effects data were compiled or developed by the manufacturers for the EPA's HPV Challenge progra...

148

TOXICOLOGY OF MONO- AND DI-ALKYLTIN CHLORIDES  

EPA Science Inventory

Mono- and di-alkyltin chlorides are reactive compounds used in the production of stabilizers for polyvinyl chloride (PVC) plastics, primarily used for water distribution pipes. Health effects data were compiled or developed by the manufacturers for the EPA's HPV Challenge progra...

149

Conditional Transgenesis Using Dimerizable Cre (DiCre)  

PubMed Central

Cre recombinase is extensively used to engineer the genome of experimental animals. However, its usefulness is still limited by the lack of an efficient temporal control over its activity. We have recently developed a conceptually new approach to regulate Cre recombinase, that we have called Dimerizable Cre or DiCre. It is based on splitting Cre into two inactive moieties and fusing them to FKBP12 (FK506-binding protein) and FRB (binding domain of the FKBP12-rapamycin associated protein), respectively. These latter can be efficiently hetero-dimerized by rapamycin, leading to the reinstatement of Cre activity. We have been able to show, using in vitro approaches, that this ligand-induced dimerization is an efficient way to regulate Cre activity, and presents a low background activity together with a high efficiency of recombination following dimerization. To test the in vivo performance of this system, we have, in the present work, knocked-in DiCre into the Rosa26 locus of mice. To evaluate the performance of the DiCre system, mice have been mated with indicator mice (Z/EG or R26R) and Cre-induced recombination was examined following activation of DiCre by rapamycin during embryonic development or after birth of progenies. No recombination could be observed in the absence of treatment of the animals, indicating a lack of background activity of DiCre in the absence of rapamycin. Postnatal rapamycin treatment (one to five daily injection, 10 mg/kg i.p) induced recombination in a number of different tissues of progenies such as liver, heart, kidney, muscle, etc. On the other hand, recombination was at a very low level following in utero treatment of DiCre×R26R mice. In conclusion, DiCre has indeed the potentiality to be used to establish conditional Cre-deleter mice. An added advantage of this system is that, contrary to other modulatable Cre systems, it offers the possibility of obtaining regulated recombination in a combinatorial manner, i.e. induce recombination at any desired time-point specifically in cells characterized by the simultaneous expression of two different promoters.

Jullien, Nicolas; Goddard, Isabelle; Selmi-Ruby, Samia; Fina, Jean-Luc; Cremer, Harold; Herman, Jean-Paul

2007-01-01

150

Technetium complexes of 3,5-di-tert-butylcatechol. Direct synthesis of tris(3,5-di-tert-butylcatecholato)technetium(VI) and bis(3,5-di-tert-butylcatecholato)-(di-tert-butylamidophenolato)technetium(VI) from ammonium pertechnetate  

SciTech Connect

The reaction between 3,5-di-tert-butylcatechol and ammonium pertechnetate in methanol produces a mixture of tris(3,5-di-tert-butylcatecholato)technetium(VI) (Tc(DBCat){sub 3}) and bis(3,5-di-tert-butylcatecholato)(di-tert-butylamido-phenolato)technetium(VI) (Tc(DBCat){sub 2}(DBAP)). The amidophenolate ligand of Tc(DBCat){sub 2}(DBAP) appears to result from Schiff-base condensation of ammonia from ammonium ion with catechol. When the reaction is carried out in the present of excess, 3,5-di-tert-butylcatechol only small quantities of Tc(DBCat){sub 2}(DBAP) are formed. Crystallographic characterization of Tc(DBCat){sub 3} has shown that the molecule crystallizes in the monoclinic space group P2{sub 1}/n in a unit cell of dimensions a = 15.892 (3) {angstrom}, b = 15.878 (4) {angstrom}, c = 16.367 (3) {angstrom}, {beta} = 93.13 (1){degree}, and V = 4123 (1) {angstrom}{sup 3}. The complex molecule is of C{sub 3} symmetry and the average ligand C-O bond length of 1.334 (13) {angstrom} is typical of values found for catecholate ligands. As such, the complex is a unique example of Tc(VI). Both Tc(DBCat){sub 3} and Tc(DBCat){sub 2}(DBAP) exhibit well-resolved 10-line EPR spectra in solution at room temperature. Coupling to the {sup 99}Tc nucleus (I = 9/2) of approximately 140 G is consistent with the d{sup 1} Tc(VI) formulation for both complexes. They each undergo reversible one-electron oxidations to cationic complexes with surprising ease, and they each undergo two reversible one-electron reductions to Tc(V) and Tc(IV) forms of the complexes.

deLearie, L.A.; Haltiwanger, R.C.; Pierpont, C.G. (Univ. of Colorado, Boulder (USA))

1989-06-07

151

Pseudosymmetric fac-di-aqua-trichlorido[(di-methyl-phosphor-yl)methanaminium-?O]manganese(II)  

PubMed Central

In the title compound, [Mn(C3H11NOP)Cl3(H2O)2], the MnII metal center has a distorted o­cta­hedral geometry, coordinated by the three chloride ligands showing a facial arrangement. Two water mol­ecules and the O-coordinated dpmaH cation [dpmaH = (di­methyl­phosphor­yl)methanaminium] complete the coordination sphere. Each complex mol­ecule is connected to its neighbours by O—H?Cl and N—H?Cl hydrogen bonds. Two of the chloride ligands and the two water ligands form a hydrogen-bonded polymeric sheet in the ab plane. Furthermore, these planes are connected to adjacent planes by hydrogen bonds from the aminium function of cationic dpmaH ligand. A pseudo-mirror plane perpendicular to the b axis in the chiral space group P21 is observed together with inversion twinning [ratio = 0.864?(5):0.136?(5)].

Reiss, Guido J.

2013-01-01

152

Caloria o Joule? Riserve nei confronti di un nuovo indirizzo metrologico  

Microsoft Academic Search

\\u000a Conclusioni  1)|In linea di massima e sul piano teorico possibile e legittimo esprimere la quantit di calore in unit di energia (e\\u000a suoi multipli);\\u000a \\u000a 2)|l’unit di energia pi consona a tale scopo quella prescelta dal SI, e cio il joule;\\u000a \\u000a \\u000a 3)|ma l’unificazione delle unit di misura, e in particolare l’adozione radicale del SI, indispensabile nella scienza \\u000a pura , non nella tecnica

Emanuele Djalma Vitali

1974-01-01

153

Allosteric activation of exopolysaccharide synthesis through cyclic di-GMP-stimulated protein-protein interaction.  

PubMed

In many bacterial pathogens, the second messenger c-di-GMP stimulates the production of an exopolysaccharide (EPS) matrix to shield bacteria from assaults of the immune system. How c-di-GMP induces EPS biogenesis is largely unknown. Here, we show that c-di-GMP allosterically activates the synthesis of poly-?-1,6-N-acetylglucosamine (poly-GlcNAc), a major extracellular matrix component of Escherichia coli biofilms. C-di-GMP binds directly to both PgaC and PgaD, the two inner membrane components of the poly-GlcNAc synthesis machinery to stimulate their glycosyltransferase activity. We demonstrate that the PgaCD machinery is a novel type c-di-GMP receptor, where ligand binding to two proteins stabilizes their interaction and promotes enzyme activity. This is the first example of a c-di-GMP-mediated process that relies on protein-protein interaction. At low c-di-GMP concentrations, PgaD fails to interact with PgaC and is rapidly degraded. Thus, when cells experience a c-di-GMP trough, PgaD turnover facilitates the irreversible inactivation of the Pga machinery, thereby temporarily uncoupling it from c-di-GMP signalling. These data uncover a mechanism of c-di-GMP-mediated EPS control and provide a frame for c-di-GMP signalling specificity in pathogenic bacteria. PMID:23202856

Steiner, Samuel; Lori, Christian; Boehm, Alex; Jenal, Urs

2012-11-30

154

Truncated form of the title: Mechanism of c-di-GMP signaling  

PubMed Central

Summary Cyclic dimeric guanosine monophosphate (c-di-GMP) is a common, bacterial second messenger that regulates diverse cellular processes in bacteria. Opposing activities of diguanylate cyclases (DGCs) and phosphodiesterases (PDEs) control c-di-GMP homeostasis in the cell. Many microbes have a large number of genes encoding DGCs and PDEs that are predicted to be part of c-di-GMP signaling networks. Other building blocks of these networks are c-di-GMP receptors which sense the cellular levels of the dinucleotide. C-di-GMP receptors form a more diverse family, including various transcription factors, PilZ domains, degenerate DGCs or PDEs, and riboswitches. Recent studies revealing the molecular basis of c-di-GMP signaling mechanisms enhanced our understanding of how this molecule controls downstream biological processes and how c-di-GMP signaling specificity is achieved.

Sondermann, Holger; Shikuma, Nicholas J.; Yildiz, Fitnat H.

2012-01-01

155

You've come a long way: c-di-GMP signaling.  

PubMed

Cyclic dimeric guanosine monophosphate (c-di-GMP) is a common, bacterial second messenger that regulates diverse cellular processes in bacteria. Opposing activities of diguanylate cyclases (DGCs) and phosphodiesterases (PDEs) control c-di-GMP homeostasis in the cell. Many microbes have a large number of genes encoding DGCs and PDEs that are predicted to be part of c-di-GMP signaling networks. Other building blocks of these networks are c-di-GMP receptors which sense the cellular levels of the dinucleotide. C-di-GMP receptors form a more diverse family, including various transcription factors, PilZ domains, degenerate DGCs or PDEs, and riboswitches. Recent studies revealing the molecular basis of c-di-GMP signaling mechanisms enhanced our understanding of how this molecule controls downstream biological processes and how c-di-GMP signaling specificity is achieved. PMID:22226607

Sondermann, Holger; Shikuma, Nicholas J; Yildiz, Fitnat H

2012-01-05

156

[Activity of cyclic diguanylate (c-di-GMP) in bacteria and the study of its derivatives].  

PubMed

Cyclic diguanylate (c-di-GMP) is a ubiquitous second messenger present in a wide variety of bacteria, which is responsible for cell differentiation, biofilm formation, pathogenic factor generation, and so on. The level of c-di-GMP in bacteria is regulated by two opposing active domains, diguanylate cyclase (DGC) and phosphodiesterase (PDE), which are present in the same bifunctional protein, and in charge of the synthesis and the degradation of c-di-GMP, respectively. The target of c-di-GMP in the bacterial cell consists of PilZ domain and GEMM riboswitch, the only riboswitch that involved in signal transduction. This article gives an overview of c-di-GMP, focusing on its metabolic pathway, regulatory mechanism, biological function of c-di-GMP, and the synthesis of c-di-GMP analogues and their biological activity. PMID:22645753

Na, Lu-Xin; Yang, Zhen-Jun

2012-03-01

157

Periodici in Scambio con gli 'Annali dell'Istituto Superiore di Sanita': Quale Ruolo in Epoca di Open Access (Periodicals in Exchange with 'Annali dell'Istituto Superiore di Sanita': Which Role in the Open Access Era).  

National Technical Information Service (NTIS)

This report covers the detailed review work done by the Editorial Unit of the Istituto Superiore di Sanita (ISS) on all the journals received through its exchange programme with the quarterly periodical Annali dellIstituto Superiore di Sanita. It gives a ...

F. Napolitani L. Radiciotti M. G. Corsi

2007-01-01

158

Photodegradation of polymer-dispersed perylene di-imide dyes  

NASA Astrophysics Data System (ADS)

Polymer-dispersed perylene di-imide dye photodegradation is investigated by monitoring the fluorescence intensity as a function of 532 nm laser pulses. Anaerobically irradiated polymer-dye films exhibited an accelerated decrease in fluorescence intensity, which was partially recovered upon exposure to oxygen. Decelerated photodegradation rates were observed for perylene di-imide ethanol solutions upon the addition of a singlet oxygen quenching antioxidant. These observations suggest reversible photoreduction and type II photo-oxidation as important photodegradation mechanisms. Type II photo-oxidation for perylene red 532 nm irradiation is supported by a singlet oxygen quantum yield of 0.09±0.03, determined via detection of time-resolved O2 (a1?g —> X3?g-) infrared phosphorescence.

Tanaka, Nobuaki; Barashkov, Nikolay; Heath, Jerry; Sisk, Wade N.

2006-06-01

159

Photodegradation of polymer-dispersed perylene di-imide dyes.  

PubMed

Polymer-dispersed perylene di-imide dye photodegradation is investigated by monitoring the fluorescence intensity as a function of 532 nm laser pulses. Anaerobically irradiated polymer-dye films exhibited an accelerated decrease in fluorescence intensity, which was partially recovered upon exposure to oxygen. Decelerated photodegradation rates were observed for perylene di-imide ethanol solutions upon the addition of a singlet oxygen quenching antioxidant. These observations suggest reversible photoreduction and type II photo-oxidation as important photodegradation mechanisms. Type II photo-oxidation for perylene red 532 nm irradiation is supported by a singlet oxygen quantum yield of 0.09+/-0.03, determined via detection of time-resolved O2 (a1delta(g) --> X3sigma(g)-) infrared phosphorescence. PMID:16724148

Tanaka, Nobuaki; Barashkov, Nikolay; Heath, Jerry; Sisk, Wade N

2006-06-01

160

Building Dust Grain Models for Di?erent ISM Environments  

NASA Astrophysics Data System (ADS)

Interstellar dust is a vital component of Galactic evolution, crucial to forming objects as small as H2 and as large as stars. Despite this, relatively little is known about the grains nature. Current grain models of the standard di?use ISM , based on constraints from the NIR-UV regimes and assumed cosmic elemental abundances, cannot be applied to X-ray observations. New grain models that are constrained by data in the full NIR-UV-X-ray bandpasses, over a broad range of dust environments, are needed. X-ray dust halos from absorbed sources observed with XMM-Newton/EPIC are an excellent diagnostic of grain characteristics that can be used in conjunction with NIR-UV data to forge a comprehensive, realistic grain model that spans these regimes in both the dense and di?use ISM.

Valencic, Lynne

2008-10-01

161

Di-muons near the Upsilon resonances in ATLAS  

NASA Astrophysics Data System (ADS)

We present measurements of the production cross-section of Upsilon mesons with di-muon final state in pp collision at a 7 TeV center-of-mass energy. The data were collected with the ATLAS detector at CERN with a corresponding integrated luminosity of 1.85 fb-1. We also report a search for a very light CP-odd Higgs boson, a 1, decaying to di-muon pairs. Such a light Higgs boson is predicted in Next-to-Minimal extensions of the Supersymmetric Standard Model (NMSSM). We set limits on the production cross-section times branching ratio for a1 masses of 6 -- 9 GeV and 11 -- 12 GeV, avoiding the region dominated by the Upsilon resonances.

Yang, Yi

162

Applicazione dell'Indice di Funzionalità Fluviale (IFF) ad un fiume minore della pianura trevigiana: il Meolo  

Microsoft Academic Search

Riassunto Nel corso dell'anno 2003 la Provincia di Treviso in collaborazione con l'Università Ca' Foscari di Venezia ha svolto una campagna di applicazione dell'Indice di Funzionalità Fluviale (IFF) all'asta del Fiume Meolo. Obiettivo della ricerca è stato quello di ampliare le conoscenze relative al fiume attraverso una prima individuazione dei fattori che incidono maggiormente sulla funzionalità dell'ecosistema fluviale e l'identificazione

Ornella De Ros; Mara Zanette; Maurizio Siligardi; Pier Francesco Ghetti; Paolo Negri; Paola Camuccio

163

INSEGNARE A TRADURRE: SPUNTI DALL'ESPERIENZA IN AMBITO LINGUISTICO E APPORTO DELLE NUOVE TECNOLOGIE. IL JOURNAL DI UN CASO1  

Microsoft Academic Search

INTRODUZIONE Lo scopo di questo contributo è quello di insegnare ad insegnare a tradurre attraverso una metodologia tradizionale che cerca, però, anche di avvalersi dell'apporto delle nuove tecnologie, operando delle riflessioni su un corso di traduzione organizzato presso l'Università Suor Orsola Benincasa di Napoli ed erogato in modalità blended. Tali riflessioni sono anticipate dall'esposizione di una serie di presupposti teorici

EMILIA DI MARTINO; Orsola Benincasa

164

Bootstrapsp eci!cation tests for di#usion processes  

Microsoft Academic Search

Abstract This paper discusses speci!cation tests for di#usion processes. In the one-dimensional case, our proposed test is closest to the nonparametric test of A2 3t-Sahalia (Rev. Financ. Stud. 9 (1996) 385). However, we compare CDFs instead of densities. In the multidimensional and\\/or multifactor case, our proposed test is based on comparison of the empirical CDF of actual data and the

Valentina Corradi; Norman R. Swanson

165

NUOVI RITROVAMENTI DI PITTURE RUPESTRI E GRAFFITI IN ERITREA  

Microsoft Academic Search

Nuove pitture e graffiti rupestri, rappresentanti animali e esseri umani eseguiti secondo tipi differenti di disegno, sono stati ritrovati, dal 1961 al 1964, nelle zone del T?m?zz?? (Ser?y?), Deggi'?n e Merett?? Sebene (Akkelè Guzáy). Fra gli animali compare, per la prima volta nelle figurazioni rupestri dell'Eritrea, anche la giraffa. De nouvelles peintures et gravures rupestres ont été découvertes, pendant les

VINCENZO FRANCHINI

1964-01-01

166

[Dr Lorenzo Dojmi di Delupis- the "Leopard" from Vis].  

PubMed

Dr Lorenzo Dojmi di Delupis (1845-1927) was a descendant of a respectful aristocratic family from Vis. His father was Peter Dojmi di Delupis (1809-1886), an attorney, a moderate pro-Italian autonomist, and a mayor of Vis, and his mother was Margherita Siminati. As soon as he graduated from the Medical University of Graz in 1870, he joined the Ottoman army as a physician for a two-year journey in which he reached as far as Baghdad and Basra. He left notes about this dramatic, juvenile adventure in his diaries. In 1878, he married a Viennese girl Maria Neidl and brought her home to Vis, where he got a position of municipal doctor. He had an extraordinary diagnosing acuity, great sympathy for patients, and was always willing to help, which is why he was adored by all the people of Vis, whether they agreed with his autonomist ideas or not, and soon became "the father of Vis". He was the first to warn of leprosy in Dalmatia, when he diagnosed two cases in Vis. Beside medicine and politics, he was also a passionate botanist. He was the first to seed palms in Vis and to grow a palm nursery with his agronomist son Peter Dojmi, for the whole of Dalmatia. He also tried breeding ostriches and growing Mexican coffee. He funded a small meteorological station in Vis, and planned to open a health resort with professor Schröter from Vienna. Even though Lorenzo Dojmi di Delupis held the great Italian culture very dear, his vocation as a physician and humanist inclination never let him burn with the fervour of a political extremist. Thanks to his psychophysical stability, he overcame numerous difficulties in life and retained faith in people. All these traits evoke Prince Salina, the leading character of the renowned novel Il gattopardo (The Leopard) by Giuseppe Tomasi di Lampedusa, which is why the author has so entitled this article "The Gattopardo of Vis". PMID:22292541

Jamnicki Dojmi, Mirko

2011-01-01

167

Di-jet production by double pomeron exchange in CDF  

SciTech Connect

We have studied events with a high-x{sub F} antiproton and two central jets with E{sub T} > 7 GeV in CDF, in p{anti p} collisions at {radical}s = 1800 GeV. We find an excess of events with a rapidity gap at least 3.5 units wide in the proton direction, which we interpret as di-jet production in double pomeron exchange events.

Albrow, M.G.

1998-05-01

168

Synthesis and characterization of di-disubstituted phthalocyanines  

Microsoft Academic Search

An improved approach to the synthesis of di-disubstituted phthalocyanines from two different phthalyl precursors is described. The method combines substituted 1,3 diiminoisoindoles and 6\\/7-nitro-1,3,3-trichloroisoindolenine to synthesize phthalocyanine. The method can be applied to the synthesis of hydrogen and metallo phtahlocyanine. The yields are variable, ranging from 17% to 72% depending on the substituents.

Joseph G. Young; William Onyebuagu

1990-01-01

169

Tephrochronology of core C from Lago Grande di Monticchio  

Microsoft Academic Search

Tephrochronological studies of sediment from Lago Grande di Monticchio have begun with detailed electron microprobe analyses\\u000a of two thickest tephra layers found in Core C. A total of 76 analyses of both layers reveals them to be of trachytic composition,\\u000a of the type erupted from the Campi Flegrei during the last 35,000 years. LGM C41, the lower layer, is a

Anthony J. Newton; Andrew J. Dugmore

170

Spectrophotometric Determination of Cobalt with Di2-Pyridyl Ketone Benzoylhydrazone  

Microsoft Academic Search

A method for the spectrophotometric determination of cobalt (II) by complexation with di-2-pyridyl ketone benzoylhydrazone (DPKBH) in 50% (v\\/v) ethanolic solution is described. Cobalt (II) forms a 1:2 complex with DPKBH. The complex has maximum absorbance at 370 nm. Beer's law is obeyed over the range 0.1 – 2.8 ?g.ml. The effect of pH, effect of excess reagent, stability of

Nidal A. Zatar; Mohammed A. Al-Nuri; Maher Abu-Eid; Mohammed Hannoun; All Z. Abu-Zuhri; Shukri Khalaf; Mustafa Khamis

1991-01-01

171

Integrated modelling in coastal lagoons: Sacca di Goro case study  

Microsoft Academic Search

A coupled 3D hydrodynamic-biogeochemical model was developed and implemented for the Sacca di Goro coastal lagoon. The model\\u000a considers nutrient and oxygen dynamics in water column and sediments. Among the biological elements, phytoplankton, zooplankton,\\u000a bacteria, Ulva sp. and commercial shellfish (Tapes philippinarum) were taken into consideration. Nutrients fluxes from the watershed and open sea, as well as atmospheric inputs, heat

D. Marinov; J. M. Zaldívar; A. Norro; G. Giordani; P. Viaroli

2008-01-01

172

Ethnic diVerences in unemployment and ill health  

Microsoft Academic Search

Objective The aim of the study is to evaluate whether health inequalities associated with unemployment are comparable across diVerent ethnic groups. Method A random sample of inhabitants of the city of Rotterdam Wlled out a questionnaire on health and its determinants, with a response of 55.4% (n = 2,057). In a cross-sectional design the associations of unemployment, ethnicity, and individual

Merel Schuring; Alex Burdorf; Anton Kunst; Toon Voorham; Johan Mackenbach

2009-01-01

173

Magnetic order in acmite: Ac81Di19  

NASA Astrophysics Data System (ADS)

Mössbauer spectra of 57Fe in acmite (Ac81Di19) have been obtained from 2 to 300 K. The natural acmite orders antiferromagnetically at 15 K. Susceptibility data in the paramagnetic region show a Curie-Weiss law behavior with Peff = 6.12?B and ? = -36 K. The Mössbauer spectrum of acmite at 2 K is interpreted as due to different environmental magnetic configurations of Fe3+.

de Oliveira, J. C. P.; da Costa, M. I.; Schreiner, W. H.; Vasquez, A.; Vieira, N.; Roisenberg, A.

1988-11-01

174

Cis-4A,5,8,8A-Tetrahydro-6,7-di-(Bromomethyl) Naphthoquinone from 2,3-di-(Bromomethyl)-1,3-Butadiene.  

National Technical Information Service (NTIS)

3,4-di-(bromomethyl)-2,5-dihydrothiophene-1,1-dioxide was prepared and then decomposed by heating to yield 2,3-di-(bromomethyl)-1,3-butadiene (I). This diene was very sensitive to light and polymerized readily. Polymerization also occurs in most solvents ...

G. B. Butler R. M. Ottenbrite

1967-01-01

175

Vagal efferent and afferent innervation of the rat esophagus as demonstrated by anterograde DiI and DiA tracing: Focus on myenteric ganglia  

Microsoft Academic Search

Anterograde tracing with the carbocyanine tracer DiI and the aminostyrol derivative DiA was used to selectively label fibers from the nucleus ambiguus, dorsal motor nucleus and nodose ganglion, respectively, terminating in the rat esophagus, and to compare them with the innervation of the gastric fundus in the same animals. Ambiguus neurons terminated on motor endplates distributed mainly to the ipsilateral

Winfried L Neuhuber; Michael Kressel; Andrea Stark; Hans-Rudolf Berthoud

1998-01-01

176

DiGeorge syndrome with vertebral and rib dysplasia  

SciTech Connect

DiGeorge syndrome results from defect in the development of the third and fourth pharyngeal pouches, and is characterized by conotruncal heart defects, aplasia or hypoplasia of thymus and parathyroid glands resulting in immune deficiency and hypocalcemia. Other associated abnormalities include renal, thyroid and diaphragmatic defects, oral clefting, etc. Etiologically, it is heterogeneous, with a microdeletion of 22q11 present in over 80% of cases. Our patient was born following a pregnancy complicated by insulin dependent gestational diabetes. There was truncus arteriosus type 2, absense of thymic shadow on CXR with severe deficiency of T cell function, and persistent hypocalcemia with low parathormone. Right kidney was absent. Dysplastic ribs including fused and bifid ribs were noted. Hypoplastic vertebrae and hemivertebrae were present through thoracic and lumbar regions. Chromosome analysis was normal, and metaphase FISH analysis with probe N25 representing locus D22S75 did not show any deletion of 22q11.2. The skeletal findings similar to these have not been previously reported in association with DiGeorge syndrome to our knowledge. Vertebral and rib abnormalities are known to occur with pregestational maternal diabetes. Maternal diabetes has also been suggested to be a possible etiology in a very small proportion of DiGeorge syndrome cases. It is possible that these findings occured together on account of gestational maternal diabetes in our case.

Puno-Cocuzza, C.; David, K.; Kogekar, N. [Brooklyn Hospital Center, NY (United States)

1994-09-01

177

DiGeorge Syndrome: a not so rare disease  

PubMed Central

INTRODUCTION: The DiGeorge Syndrome was first described in 1968 as a primary immunodeficiency resulting from the abnormal development of the third and fourth pharyngeal pouches during embryonic life. It is characterized by hypocalcemia due to hypoparathyroidism, heart defects, and thymic hypoplasia or aplasia. Its incidence is 1:3000 live births and, despite its high frequency, little is known about its natural history and progression. ?This is probably due to diagnostic difficulties and the great variety of names used to describe it, such as velocardiofacial, Shprintzen, DiGeorge, and CATCH 22 Syndromes, as well as conotruncal facial anomaly. All represent the same genetic condition, chromosome 22q11.2 deletion, which might have several clinical expressions. OBJECTIVES: To describe clinical and laboratorial data and phenotypic characteristics of patients with DiGeorge Syndrome. METHODS: Patients underwent standard clinical and epidemiological protocol and tests to detect heart diseases, facial abnormalities, dimorphisms, neurological or behavioral disorders, recurrent infections and other comorbidities. RESULTS: Of 14 patients (8m – 18y11m), only one did not have 22q11.2 deletion detected. The main findings were: conotruncal malformation (n ?=? 12), facial abnormalities (n ?=? 11), hypocalcemia (n ?=? 5) and low lymphocyte count (n?=?2). CONCLUSION: The authors pointed out the necessity of DGS suspicion in all patient presenting with heart defects, facial abnormalities (associated or not with hypocalcemia), and immunological disorders because although frequency of DGS is high, few patients with a confirmed diagnosis are followed up.

Fomin, Angela BF; Pastorino, Antonio Carlos; Kim, Chong Ae; Pereira, Alexandre C; Carneiro-Sampaio, Magda; Abe Jacob, Cristina Miuki

2010-01-01

178

Endo-S-c-di-GMP analogues-polymorphism and binding studies with class I riboswitch.  

PubMed

C-di-GMP, a cyclic guanine dinucleotide, has been shown to regulate biofilm formation as well as virulence gene expression in a variety of bacteria. Analogues of c-di-GMP have the potential to be used as chemical probes to study c-di-GMP signaling and could even become drug leads for the development of anti-biofilm compounds. Herein we report the synthesis and biophysical studies of a series of c-di-GMP analogues, which have both phosphate and sugar moieties simultaneously modified (called endo-S-c-di-GMP analogues). We used computational methods to predict the relative orientation of the guanine nucleobases in c-di-GMP and analogues. DOSY NMR of the endo-S-c-di-GMP series showed that the polymorphism of c-di-GMP can be tuned with conservative modifications to the phosphate and sugar moieties (conformational steering). Binding studies with Vc2 RNA (a class I c-di-GMP riboswitch) revealed that conservative modifications to the phosphate and 2'-positions of c-di-GMP dramatically affected binding to class I riboswitch. PMID:23143150

Zhou, Jie; Sayre, David A; Wang, Jingxin; Pahadi, Nirmal; Sintim, Herman O

2012-11-09

179

Cyclic di-GMP signaling regulates invasion by Ehrlichia chaffeensis of human monocytes.  

PubMed

Cyclic di-GMP (c-di-GMP) is a bacterial second messenger produced by GGDEF domain-containing proteins. The genome of Ehrlichia chaffeensis, an obligatory intracellular bacterium that causes human monocytic ehrlichiosis, encodes a single protein that contains a GGDEF domain, called PleD. In this study, we investigated the effects of c-di-GMP signaling on E. chaffeensis infection of the human monocytic cell line THP-1. Recombinant E. chaffeensis PleD showed diguanylate cyclase activity as it generated c-di-GMP in vitro. Because c-di-GMP is not cell permeable, the c-di-GMP hydrophobic analog 2'-O-di(tert-butyldimethylsilyl)-c-di-GMP (CDGA) was used to examine intracellular c-di-GMP signaling. CDGA activity was first tested with Salmonella enterica serovar Typhimurium. CDGA inhibited well-defined c-di-GMP-regulated phenomena, including cellulose synthesis, clumping, and upregulation of csgD and adrA mRNA, indicating that CDGA acts as an antagonist in c-di-GMP signaling. [(32)P]c-di-GMP bound several E. chaffeensis native proteins and two E. chaffeensis recombinant I-site proteins, and this binding was blocked by CDGA. Although pretreatment of E. chaffeensis with CDGA did not reduce bacterial binding to THP-1 cells, bacterial internalization was reduced. CDGA facilitated protease-dependent degradation of particular, but not all, bacterial surface-exposed proteins, including TRP120, which is associated with bacterial internalization. Indeed, the serine protease HtrA was detected on the surface of E. chaffeensis, and TRP120 was degraded by treatment of E. chaffeensis with recombinant E. chaffeensis HtrA. Furthermore, anti-HtrA inhibited CDGA-induced TRP120 degradation. Our results suggest that E. chaffeensis invasion is regulated by c-di-GMP signaling, which stabilizes some bacterial surface-exposed proteins against proteases. PMID:20562302

Kumagai, Yumi; Matsuo, Junji; Hayakawa, Yoshihiro; Rikihisa, Yasuko

2010-06-18

180

(Di-methyl-phosphor-yl)methanaminium iodide-(di-methyl-phosphor-yl)methan-amine (1/1)  

PubMed Central

The asymmetric unit of the title structure, C3H11NOP+·I?·C3H10NOP, consists of one (di­methyl­phosphor­yl)methanamine (dpma) mol­ecule, one (di­methyl­phosphor­yl)methanaminium (dpmaH) ion and one iodide counter-anion. In the crystal, medium–strong to weak N—H?O and N—H?N hydrogen bonds connect dpmaH cations and dpma mol­ecules into strands along [001]. The iodide counter-anions form only very weak hydrogen bonds. The crystal used for the diffraction study was found to be an inversion twin with a ratio of 0.83?(2):0.17?(2). The title structure is isotypic to that of dpmaH[ClO4]·dpma [Buhl et al. (2013 ?). Crystals 3, 350–362].

Reiss, Guido J.

2013-01-01

181

Lo que Usted Necesita Saber Sobre el Mercurio en el ...  

Center for Food Safety and Applied Nutrition (CFSAN)

... los EE.UU. en el 1-888-SAFEFOOD o visite el sitio Web de la FDA sobre la seguridad de los alimentos. Para más información ... More results from www.fda.gov/food/foodborneillnesscontaminants/buystoreservesafefood

182

Cost-effective isobaric tagging for quantitative phosphoproteomics using DiART reagents.  

PubMed

We describe the use of an isobaric tagging reagent, Deuterium isobaric Amine Reactive Tag (DiART), for quantitative phosphoproteomic experiments. Using DiART tagged custom mixtures of two phosphorylated peptides from alpha casein and their non-phosphorylated counterparts, we demonstrate the compatibility of DiART with TiO2 affinity purification of phosphorylated peptides. Comparison of theoretical vs. experimental reporter ion ratios reveals accurate quantification of phosphorylated peptides over a dynamic range of more than 15-fold. Using DiART labelling and TiO2 enrichment (DiART-TiO2) with large quantities of proteins (8 mg) from the cell lysate of model fungus Aspergillus nidulans, we quantified 744 unique phosphopeptides. Overlap of median values of TiO2 enriched phosphopeptides with theoretical values indicates accurate trends. Altogether these findings confirm the feasibility of performing quantitative phosphoproteomic experiments in a cost-effective manner using isobaric tagging reagents, DiART. PMID:24129742

Ramsubramaniam, Nikhil; Tao, Feng; Li, Shuwei; Marten, Mark R

2013-10-16

183

Boschi frammentati nei territori comasco, lecchese e milanese: problematiche fitosociologiche e stato di conservazione  

Microsoft Academic Search

RIASSUNtO - Boschi frammentati nei territori comasco, lecchese e milanese: problematiche fitosociologiche e stato di conservazione - È stata condotta una ricerca in ambito ecologico e fitosociologico sui boschi dei cordoni morenici delle province di Como, Lecco e Milano (Brianza), considerevolmente frammentati per l'intenso sfruttamento antropico e l'espansione dei nuclei abitati. Lo studio si colloca all'interno di una ricerca più

Patrizia DIGIOVINAZZO; Carlo ANDREIS

184

Effects of di-iso-butyl phthalate on testes of prepubertal rats and mice.  

PubMed

Di-iso-butyl phthalate (DiBP), a special plasticizer, is used as a substitute for di(n-butyl) phthalate (DBP). The effects of DiBP on testes in prepubertal rodents still remain to be obscure. Testicular toxicity of DiBP was investigated in 21-day-old Sprague-Dawley rats and C57BL/6N mice, using with in situ TUNEL method. For an acute exposure experiment, animals were once given DiBP at various concentrations by oral gavage. For a subchronic exposure experiment, they were daily given DiBP at various concentrations for consecutive 7 days. Controls were treated with corn oil under the same condition. For a recovery experiment, rats were once given DiBP (1000 mg/kg), and were sacrificed at day 1 to 8 after administration. Furthermore, the disorder of vimentin filaments in Sertoli cells after daily administration of DiBP (500 mg/kg) for consecutive 7 days in rats also identified by immunohistochemistry using anti-vimentin antibody. As a result, the present study demonstrated that DiBP can induce testicular atrophy in rats due to the increase of TUNEL-positive spermatogenic cells in both acute and subchronic exposure experiments. At the same time, the disorder of vimentin filaments in Sertoli cells was recognized. However, no such damages could be found in mouse testis. For the recovery experiment, the testis weight and testicular morphology returned to normal at day 6 after administration. In conclusion, the present study indicates that DiBP causes the significant increase of TUNEL-positive spermatogenic cells and the disorder of vimentin filaments in Sertoli cells in rats and that DiBP shows a species-specific toxicity. PMID:20560449

Zhu, Xiao Bo; Tay, Tat Wei; Andriana, Bibin Bintang; Alam, Mohammad Shah; Choi, Ehn Kyoung; Tsunekawa, Naoki; Kanai, Yoshiakira; Kurohmaru, Masamichi

2010-02-01

185

Metabolismo di cellule adipose umane isolate in vitro. I. Effetto del glucosio e dell'Insulina  

Microsoft Academic Search

Riassunto L'insulina stimola l'incorporazione dell' 114C del glucosio nel CO2 prodotto da cellule adipose umane isolatein vitro. Tale effetto sembra essere evidente solo con concentrazioni abbastanza elevate dell'ormone, nelle condizioni sperimentali descritte nel presente lavoro. Analogamente, anche l'effetto antilipolitico dell'insulina è evidenziabile, in modesta entità, solo con concentrazioni relativamente elevate dell'ormone nelmedium di incubazione. La presenza di glucosio nelmedium di

Guido Pozza; Alberto Ghidoni; Edda Sanesi; Augusta Pappalettera; Angela Tognetti

1970-01-01

186

Structural and Biochemical Determinants of Ligand Binding by the c-di-GMP Riboswitch  

Microsoft Academic Search

The bacterial second messenger c-di-GMP is used in many species to control essential processes that allow the organism to adapt to its environment. The c-di-GMP riboswitch (GEMM) is an important downstream target in this signaling pathway and alters gene expression in response to changing concentrations of c-di-GMP. The riboswitch selectively recognizes its second messenger ligand primarily through contacts with two

Kathryn D. Smith; Sarah V. Lipchock; Alison L. Livingston; Carly A. Shanahan; Scott A. Strobel

2010-01-01

187

Migrazione a Software Open Source nella Pubblica Amministrazione: Un Caso di Studio Empirico  

Microsoft Academic Search

L'elaborato presenta la valutazione di una migrazione ad un Software Open Source (OSS) in una Pubblica Amministrazione. In particolare, l'ambito considerato è stato quello critico di office automation, con l'introduzione della suite OpenOffice.org. Per la valutazione, sono stati raccolti dati qualitativi e quantitativi tramite costante monitoraggio permesso da differenti software di raccolta dati. L'analisi è stata svolta tenendo in considerazione

Fabio Mulazzani; Bruno Rossi; Barbara Russo; Giancarlo Succi

188

Structural Basis of Ligand Binding by a C-di-GMP Riboswitch  

SciTech Connect

The second messenger signaling molecule bis-(3{prime}-5{prime})-cyclic dimeric guanosine monophosphate (c-di-GMP) regulates many processes in bacteria, including motility, pathogenesis and biofilm formation. c-di-GMP-binding riboswitches are important downstream targets in this signaling pathway. Here we report the crystal structure, at 2.7 {angstrom} resolution, of a c-di-GMP riboswitch aptamer from Vibrio cholerae bound to c-di-GMP, showing that the ligand binds within a three-helix junction that involves base-pairing and extensive base-stacking. The symmetric c-di-GMP is recognized asymmetrically with respect to both the bases and the backbone. A mutant aptamer was engineered that preferentially binds the candidate signaling molecule c-di-AMP over c-di-GMP. Kinetic and structural data suggest that genetic regulation by the c-di-GMP riboswitch is kinetically controlled and that gene expression is modulated through the stabilization of a previously unidentified P1 helix, illustrating a direct mechanism for c-di-GMP signaling.

Smith, K.; Lipchock, S; Ames, T; Wang, J; Breaker, R; Strobel, S

2009-01-01

189

The Hydrolysis of Di-Isopropyl Methylphosphonate in Ground Water  

SciTech Connect

Di-isopropyl methylphosphonate (DIMP) is a byproduct from the manufacture of the nerve agent Sarin. The persistence of DIMP in the ground water is an important question in evaluating the potential environmental impacts of DIMP contamination. The half-life of DIMP in ground water at 10 deg C was estimated to be 500 years with a 95% confidence interval of 447 to 559 years from measurements of the hydrolysis rates at temperatures between 70 to 98 deg C.Extrapolation of the kinetics to 10 deg C used the Arrhenius equation, and calculation of the half-life assumed first-order kinetics. Inorganic phosphate was not detected.

Sega, G.A., Tomkins, B.A., Griest, W.H., Bayne, C.K.

1997-12-31

190

Building a scientific data grid with DiGS.  

PubMed

We provide an insight into the challenge of building and supporting a scientific data infrastructure with reference to our experience working with scientists from computational particle physics and molecular biology. We illustrate how, with modern high-performance computing resources, even small scientific groups can generate huge volumes (petabytes) of valuable scientific data and explain how grid technology can be used to manage, publish, share and curate these data. We describe the DiGS software application, which we have developed to meet the needs of smaller communities and we have highlighted the key elements of its functionality. PMID:19451103

Beckett, Mark G; Allton, Chris R; Davies, Christine T H; Davis, Ilan; Flynn, Jonathan M; Grant, Eilidh J; Hamilton, Russell S; Irving, Alan C; Kenway, R D; Ostrowski, Radoslaw H; Perry, James T; Swedlow, Jason R; Trew, Arthur

2009-06-28

191

Di-TERT-BUTYLMETHYL-SILANOL, -SILANOLATE UND SILOXANE  

Microsoft Academic Search

Di-tert-butylfluoromethylsilane reacts with KOH to give (CMc3)2MeSiOH (1). Alkali-metal derivatives are formed in the reaction of 1 with nC4H9Li (2), sodium (3), and potassium (4). Halogen-functional 1,3-disiloxanes (5,7), 1.3.5-trisiloxanes (6,8), and a 3-bora-1.5-disiloxane (9) are obtained in the reaction of 2 with SiF4, SiCl4, and BF3, respectively. The 3,3-difluoro-1,3,5-trisiloxane 6 reacts with KOH to give the fluorosilanol 10. The condensation

Steffen Schütte; Christina Freire-Erdbrügger; Uwe Klingebiel; George M. Sheldrick

1993-01-01

192

Synthesis and study of conjugated polymers containing Di- or Triphenylamine  

SciTech Connect

This thesis consists of two separate parts. The first part addresses the synthesis and study of conjugated polymers containing di- or triphenylamine. Two types of polymers: linear polymers and dendrimers, were synthesized. The polymers were characterized by NMR, IR, UV, GPC, TGA and DSC. Electronic and optical properties of the polymers were studied through the conductivity measurements and excitation- emission spectra. the second part of this thesis deals with a reaction of electron-rich acetylenes with TCNE. The discovery of the reaction from charge transfer complex studies and the investigation of this reaction on various electron-rich acetylenes are presented.

Sukwattanasinitt, M.

1996-06-21

193

The silicon di-vacancy investigated with density functional theory  

NASA Astrophysics Data System (ADS)

As part of a larger effort to quantitatively model the behavior of point defects in silicon, we have investigated the silicon di-vacancy using density functional theory with three different exchange-correlation functionals and super-cells containing as many as 1000 atoms. The literature on this defect contains two proposed atomic structures and disagreement on the relative stability of the configurations. Our study indicates that the determination of the ground state configuration is dependant on the particular choice for exchange and correlation. We will report on the atomic and electronic structures of this defect as well as formation.

Wixom, Ryan; Wright, Alan

2006-03-01

194

The fate of di-(3,5-di-tert.-butyl-4-hydroxyphenyl)methane (Ionox 220) in the rat  

PubMed Central

1. A large proportion of a single oral dose of [14C]Ionox 220 to rats is eliminated in 24 days: 89·3–97·4% of the label is excreted in the faeces (much of this is eliminated in the first 4 days after dosage), 1% in the urine and less than 0·1% in the expired gases; 4·06% of 14C is present in the carcass and viscera after removal of the gut, and most of this is in the fatty tissues. 2. About 87% of 14C in the faeces is due to unchanged antioxidant, 5% to the quinone methide, 5% to the free acid and 3% to an unidentified polar constituent. Three-fifths of 14C in the urine is due to 3,5-di-tert.-butyl-4-hydroxybenzoic acid and the remainder to the ester glucuronide. In three individual animals, one-half of 14C in the bile is due to the free acid, one-quarter to the ester glucuronide and the remainder to unchanged antioxidant, whereas in another all of 14C in the bile is due to Ionox 220. About 97% of 14C in the body fat is due to unchanged antioxidant and the remainder to the free acid. 3. Up to 20% of a single oral dose of Ionox 220 is absorbed in rats: 13–14% is metabolized. 3,5-Di-tert.-butyl-4-hydroxybenzoic acid accounts for just over 5% of a dose of Ionox 220, 3,5-di-tert.-butyl-4-hydroxybenzoyl-?-d-glucopyranosiduronic acid for less than 0·4%, the quinone methide for just over 5% and an unidentified compound for less than 3%. 4. The physiological and biochemical implications of ingesting Ionox 220 are discussed.

Wright, A. S.; Crowne, R. S.; Hathway, D. E.

1966-01-01

195

Lotta alla Contraffazione Farmaceutica: Le Attivita Dell'Istituto Superiore di Sanita (Fight against Pharmacuetical Counterfeiting: The Activities of Istituto Superiore di Sanita).  

National Technical Information Service (NTIS)

Pharmaceutical counterfeiting is a serious public health problem concerning both developing and industrialized countries, but with different diffusion and characteristics. This report resumes the activities that the Istituto Superiore di Sanita (ISS, the ...

A. L. Rodomonte B. Gallinella E. Antoniella L. Fucili L. Manna L. Romanini L. Rufini L. Valvo M. Bartolomei M. DiGregorio M. Mirra M. G. Gaudiano N. Muleri P. Bertocchi S. Alimonti S. Lucattini

2010-01-01

196

Theoretical Study of the Effects of Di-Muonic Molecules on Muon- Catalyzed Fusion.  

National Technical Information Service (NTIS)

This document presents a theoretical study di-muonic hydrogen and helium molecules that have the potential of enhancing the muon-catalyzed fusion reaction rate. In order to study these di-muonic molecules a method of non- adiabatic quantum mechanics refer...

E. V. Sheely

2012-01-01

197

Aspiration pneumonia in the child with DiGeorge syndrome -A case report-  

PubMed Central

DiGeorge syndrome is associated with a chromosome 22q11.2 deletion and manifests with variable clinical findings. Aspiration pneumonia can be a perioperative complication of great concern in this syndrome. In this report, we present a case of a 16-month old child with DiGeorge syndrome undergoing cranioplasty. He developed perioperative aspiration pneumonia but was managed successfully.

Han, Yun-Joung

2011-01-01

198

Electric brain responses reveal gender di¡erences in music processing  

Microsoft Academic Search

The present study investigates gender di¡erences in the functional organization of the brain for music processing. In the language do- main, males appear to have greater left hemisphere control than females. Despite some overlap of neural structures and processes for the perception of music and language, gender di¡erences of mu- sical functions have so far not been reported. Data sets

Stefan Koelsch; CA Burkhard Maess; Tobias Grossmann; Angela D. Friederici

2003-01-01

199

A novel capture compound for the identification and analysis of cyclic di-GMP binding proteins.  

PubMed

The second messenger cyclic di-GMP is a near-ubiquitous signaling molecule that globally alters bacterial cell physiology to promote biofilm formation and community behavior. Much progress was made in recent years towards the identification and characterization of diguanylate cyclases and phosphodiersterases, enzymes involved in the synthesis and degradation of this signaling compound. In contrast, our knowledge of the nature and mechanistic details of c-di-GMP effector proteins lags behind, primarily because effective tools for their specific enrichment and rapid analysis are missing. In this report we demonstrate that a novel tri-functional c-di-GMP-specific Capture Compound (cdG-CC) can be effectively used to identify and validate c-di-GMP binding proteins. The cdG-CC was able to specifically and efficiently pull down bona fide c-di-GMP effector proteins. Furthermore, in combination with mass spectrometry (CCMS), this technology robustly identified a substantial fraction of the known c-di-GMP signaling components directly from cell extracts of different model organisms. Finally, we applied the CCMS technique to profile c-di-GMP binding proteins of Pseudomonas aeruginosa and Salmonella enterica serovar typhimurium. Our studies establish CCMS as a powerful and versatile tool to identify and analyze components of the cellular c-di-GMP pathway in a wide range of different organisms. PMID:22652488

Nesper, Jutta; Reinders, Alberto; Glatter, Timo; Schmidt, Alexander; Jenal, Urs

2012-05-29

200

Oxidation and alkoxylation of 3,6-di-tert-butylprocatechol catalyzed by manganese diacetate  

SciTech Connect

The authors have found a catalytic effect for Mn (OAc)/sub 2/ in the oxidation and alkoxylation of 3,6-di-tert-butylpyrocatechol. A mechanism is proposed entailing the formation of a metal complex with pyrocatechol and oxygen ligands. A preparative procedure is developed for the synthesis of alkoxylated derivatives of 3,6-di-tert-butyl-o-benzoquinone.

Voleva, V.B.; Ershov, V.V.; Ivanova, E.V.; Novikova, I.A.

1986-07-01

201

40 CFR 721.9675 - Titanate [Ti6O13 (2-)], di-po-tas-sium.  

Code of Federal Regulations, 2010 CFR

... 2010-07-01 false Titanate [Ti6O13 (2-)], di-po-tas-sium. 721.9675 Section 721.9675 Protection...Substances § 721.9675 Titanate [Ti6O13 (2-)], di-po-tas-sium. (a) Chemical substance and significant...

2010-07-01

202

40 CFR 721.9675 - Titanate [Ti6O13 (2-)], di-po-tas-sium.  

Code of Federal Regulations, 2013 CFR

... 2013-07-01 false Titanate [Ti6O13 (2-)], di-po-tas-sium. 721.9675 Section 721.9675 Protection...Substances § 721.9675 Titanate [Ti6 O13 (2-)], di-po-tas-sium. (a) Chemical substance and significant...

2013-07-01

203

Host Immune Response to Bacterial Cyclic Diguanylic Acid (c-di-GMP).  

National Technical Information Service (NTIS)

This work tested the hypothesis that c-di-GMP is a novel immunostimulatory molecule that modulates the immune response. Intramammary treatment of mice with c-di-GMP before S. aureus challenge gave a protective effect and a 1O,OOO-fold reduction in CFUs in...

D. K. Karaolis J. P. Nataro

2009-01-01

204

Volumi di offerta e marketing. Il caso dei prodotti agro-alimentari tipici  

Microsoft Academic Search

Volumi di offerta e marketing. Il caso dei prodotti agro-alimentari tipici (di Gervasio Antonelli) - ABSTRACT: In recent years many studies have pointed out the importance of applying business marketing techniques to firms in the agro-food sector. In the perspective attention was mainly focused on the strategies underlying traditional marketing management approaches. Thus, this paper aims to assess both extent

Gervasio Antonelli

2000-01-01

205

Nanomolar fluorescent detection of c-di-GMP using a modular aptamer strategy.  

PubMed

C-di-GMP regulates important processes involved in biofilm formation and virulence factors production in several bacteria. Herein we report a simple fluorescent strategy that allows for the detection of c-di-GMP (as low as 320 nM) using a Vc2 class I riboswitch domain as the sensing region and spinach as the fluorescent reporting module. PMID:22854718

Nakayama, Shizuka; Luo, Yiling; Zhou, Jie; Dayie, T Kwaku; Sintim, Herman O

2012-08-02

206

Quattordici Punti di accordo con i rapporti ufficiali governativi sulla distruzione del World Trade Center  

Microsoft Academic Search

I rapporti di FEMA e NIST contengono la versione ufficiale sulla distruzione del World Trade Center, avvenuta l'11 settembre 2001. In questa lettera vogliamo porre le basi per una discussione costruttiva e chiarificatrice, focalizzandoci sui punti di comune accordo con FEMA e NIST, e refutando nel contempo diverse credenze errate sui crolli del WTC. L'undici settembre 2001 le Torri Gemelle

Steven E. Jones; Frank M. Legge; Kevin R. Ryan; Anthony F. Szamboti; James R. Gourley

207

Di-n-butyl phthalate (DnBP) and diisobutyl phthalate (DiBP) metabolism in a human volunteer after single oral doses.  

PubMed

An individual (male, 36 years, 87 kg) ingested two separate doses of di-n-butyl phthalate (DnBP) and diisobutyl phthalate (DiBP) at a rate of ~60 ?g/kg. Key monoester and oxidized metabolites were identified and quantified in urine continuously collected until 48 h post-dose. For both DnBP and DiBP, the majority of the dose was excreted in the first 24 h (92.2 % of DnBP, 90.3 % of DiBP), while only <1 % of the dose was excreted in urine on day 2. In each case, the simple monoesters were the major metabolites (MnBP, 84 %; MiBP, 71 %). For DnBP, ~8 % was excreted as various side chain oxidized metabolites. For DiBP, approximately 20 % was excreted mainly as the oxidized side chain metabolite 2OH-MiBP, indicating that the extent of oxidative modification is around 2.5 times higher for DiBP than for DnBP. All DnBP and DiBP metabolites reached peak concentrations between 2 and 4 h post-exposure, followed by a monotonic decline. For DnBP metabolites, the elimination halftime of MnBP was 2.6 h; longer elimination halftimes were estimated for the oxidized metabolites (2.9-6.9 h). For DiBP metabolites, MiBP had the shortest halftime (3.9 h), and the oxidized metabolites had somewhat longer halftimes (4.1 and 4.2 h). Together with the simple monoesters, secondary oxidized metabolites are additional and valuable biomarkers of phthalate exposure. This study provides basic human metabolism and toxicokinetic data for two phthalates that have to be considered human reproductive toxicants and that have been shown to be omnipresent in humans. PMID:22820759

Koch, H M; Christensen, K L Y; Harth, V; Lorber, M; Brüning, T

2012-07-22

208

Additive property of secondary relaxation processes in di-n-octyl and di-isooctyl phthalates: Signature of non-Johari-Goldstein relaxation  

Microsoft Academic Search

Broadband dielectric spectroscopy was used to study relaxation dynamics of supercooled di-n-octyl phthalate, di-isooctyl phthalate, and their mixtures. Additionally, low temperature measurements were performed to investigate the nature of the secondary relaxation processes in both glass formers. The authors found that the secondary relaxation observed in the mixture is the additive sum of the secondary relaxations of the two components.

Ewa Kaminska; Kamil Kaminski; Marian Paluch; Jerzy Ziolo; K. L. Ngai

2007-01-01

209

Evoluzione paleoambientale dal Tardoglaciale a oggi ricostruita attraverso lo studio dei sedimenti del Lago di Lavarone (Altopiano di Folgaria e Lavarone, Trentino)  

Microsoft Academic Search

SUMMARY - Paleoenvironmental evolution from Lateglacial to present as reconstructed from Lago di Lavarone sediment sequence (Folgaria and Lavarone Plateau, Trentino) - A multi-disciplinary study of the history of Lago di Lavarone (1115 m a.s.l., southern-eastern Trentino) and its environment was undertaken based on an almost 10 m long sedimentary sequence within the framework of the OLOAMBIENT project. The study

Maria Letizia FILIPPI; Oliver HEIRI; Enrico ARPENTI; Nicola ANGELI; Mauro BORTOLOTTI; André F. LOTTER; Klaas VAN DER BORG

2007-01-01

210

32 CFR 644.336 - Notices to Departments of Interior (DI); Health and Human Resources (HHR); Education; and Housing...  

Code of Federal Regulations, 2013 CFR

...Departments of Interior (DI); Health and Human Resources...DI of land suitable for public park and recreation...purposes or to protect the public health, and to HUD of property...related facilities (Section 101-47.203.5...

2013-07-01

211

PERIPUBERTAL DI (2-ETHYLHEXYL) PHTHALATE EXPOSURE INHIBITS ANDROGEN SENSITIVE TISSUE DEVELOPMENT AND DELAYS PUBERTY IN MALE SPRAGUE-DAWLEY RATS  

EPA Science Inventory

PERIPUBERTAL DI (2-ETHYLHEXYL) PHTHALATE EXPOSURE INHIBITS ANDROGEN SENSITIVE TISSUE DEVELOPMENT AND DELAYS PUBERTY IN MALE SPRAGUE-DAWLEY RATS Nigel Noriega, Jonathan Furr, Christy Lambright, Vickie Wilson, L. Earl Gray Jr. The plasticizer Di (2-ethylhexyl) phtha...

212

Di-alkylated paromomycin derivatives: targeting the membranes of gram positive pathogens that cause skin infections.  

PubMed

A collection of paromomycin-based di-alkylated cationic amphiphiles differing in the lengths of their aliphatic chain residues were designed, synthesized, and evaluated against 14 Gram positive pathogens that are known to cause skin infections. Paromomycin derivatives that were di-alkylated with C7 and C8 linear aliphatic chains had improved antimicrobial activities relative to the parent aminoglycoside as well as to the clinically used membrane-targeting antibiotic gramicidin D; several novel derivatives were at least 16-fold more potent than the parent aminoglycoside paromomycin. Comparison between a di-alkylated and a mono-alkylated paromomycin indicated that the di-alkylation strategy leads to both an improvement in antimicrobial activity and to a dramatic reduction in undesired red blood cell hemolysis caused by many aminoglycoside-based cationic amphiphiles. Scanning electron microscopy provided evidence for cell surface damage by the reported di-alkylated paromomycins. PMID:23602621

Berkov-Zrihen, Yifat; Herzog, Ido M; Feldman, Mark; Sonn-Segev, Adar; Roichman, Yael; Fridman, Micha

2013-04-02

213

Interactions of the c-di-GMP riboswitch with its second messenger ligand.  

PubMed

The c-di-GMP [bis-(3'-5')-cyclic dimeric guanosine monophosphate] riboswitch is a macromolecular target in the c-di-GMP second messenger signalling pathway. It regulates many genes related to c-di-GMP metabolism as well as genes involved in bacterial motility, virulence and biofilm formation. The riboswitch makes asymmetric contacts to the bases and phosphate backbone of this symmetric dinucleotide. The phylogenetics suggested and mutagenesis has confirmed that this is a flexible motif where variants can make alternative interactions with each of the guanine bases of c-di-GMP. A mutant riboswitch has been designed that can bind a related molecule, c-di-AMP, confirming the most important contacts made to the ligand. The binding kinetics reveal that this is a kinetically controlled riboswitch and mutations to the riboswitch lead to increases in the off-rate. This riboswitch is therefore flexible in sequence as well as kinetic properties. PMID:21428955

Smith, Kathryn D; Strobel, Scott A

2011-04-01

214

Chronic toxicity of di-n-butyl and di-n-octyl phthalate to Daphnia magna and the fathead minnow  

SciTech Connect

The toxicities of di-n-butyl phthalate (DBP) and di-n-octyl phthalate (DOP) were assessed by measuring the effect of exposure to these compounds on the fecundity of Daphnia magna and on the hatching and survival of the early life stages of the fathead minnow Pimephales promelas. For D. magna, exposure to 1.8 mg/L DBP or 1.0 mg/L DOP caused a significant reduction in reproduction. Doses of 0.56 mg/L DBP or 0.32 mg/L DOP had no significant effect in decreasing reproduction. Survival of fathead minnow embryos was decreased by exposure to 1.8 mg/L DBP; none of the embryos exposed to this dose hatched successfully. Hatching and larval survival were affected by exposure to 1.0 mg/L DBP, but not to 0.56 mg/L. Exposure to DOP did not affect survival of either early embryos or larvae of the fathead minnow at doses up to 10 mg/L (the highest dose tested). Hatching of the embryos was significantly decreased at 10 mg/L, but not at 3.2 mg/L DOP. 26 references, 4 figures, 11 tables.

McCarthy, J.F.; Whitmore, D.K.

1985-01-01

215

Di(2-ethylhexyl)phthalate (DEHP) and di-n-butylphthalate (DBP) exposure through diet in hospital patients.  

PubMed

Ready-to-eat packed meals intended to hospital patients were studied over a two-weeks period to measure the contents of di-(2-ethylhexyl) phthalate (DEHP) and di-n-butylphthalate (DBP) and to evaluate their daily intake by total diet. The packaging consisted of polyethylene terephthalate (PET) dishes sealed with polypropylene (PP) foil. The DEHP mean concentrations in total meals varied from 0.061±0.028 to 0.307±0.138?g/gwetweight (wet wt.); the DBP mean levels varied from 0.025±0.018 to 0.174±0.091?g/gwetwt. Highest levels of concentration for DEHP and DBP were found in bread with mean values of 0.307±0.138?g/gwetwt. and 0.174±0.091?g/gwetwt. for DEHP and DBP, respectively. The daily intake for DEHP was 3.1±0.9?g/kgbw and 1.5±0.5?g/kgbw for DBP. The mean±sd incidence of DEHP and DBP intake via hospital meals on the respective EFSA TDI was 6±2% (range 4-11%), and 15±5% (range 8-24%), respectively. Even if for hospital patients the major route of exposure may be represented by medical devices, the influence of the diet could have a significant value on TDI. PMID:23108212

Cirillo, Teresa; Fasano, Evelina; Esposito, Francesco; Montuori, Paolo; Amodio Cocchieri, Renata

2012-10-26

216

Antiandrogenic effects in male rats perinatally exposed to a mixture of di(2-ethylhexyl) phthalate and di(2-ethylhexyl) adipate.  

PubMed

Di(2-ethylhexyl) phthalate (DEHP) is a well-known testicular toxicant inducing adverse effects in androgen responsive tissues. Therefore, di(2-ethylhexyl) adipate (DEHA) is currently being evaluated as a potential substitute for DEHP. Similarities in structure and metabolism of DEHP and DEHA have led to the hypothesis that DEHA can modulate the effects of DEHP. Wistar rats were gavaged with either vehicle, DEHP (300 or 750mg/kg bw/day) or DEHP (750mg/kg bw/day) in combination with DEHA (400mg/kg bw/day) from gestation day (GD) 7 to postnatal day (PND) 17. Decreased anogenital distance (AGD) and retention of nipples in male offspring were found in all three exposed groups. Dosed males exhibited decreased weights of ventral prostate and m. levator ani/bulbocavernosus. Histopathological investigations revealed alterations in testis morphology in both juvenile and adult animals. The litter size was decreased and postnatal mortality was increased in the combination group only, which is likely a combined effect of DEHP and DEHA. However, no combination effect was seen with respect to antiandrogenic effects, as males receiving DEHP in combination with DEHA did not exhibit more pronounced effects in the reproductive system than males receiving DEHP alone. PMID:15749265

Jarfelt, Kirsten; Dalgaard, Majken; Hass, Ulla; Borch, Julie; Jacobsen, Helene; Ladefoged, Ole

217

Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose  

PubMed Central

Cyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes, including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in order to exert its regulatory functions. In Salmonella, two proteins, BcsA and YcgR, both of them containing a c-di-GMP binding PilZ domain, are the only known c-di-GMP receptors. BcsA, upon c-di-GMP binding, synthesizes cellulose, the main exopolysaccharide of the biofilm matrix. YcgR is dedicated to c-di-GMP-dependent inhibition of motility through its interaction with flagellar motor proteins. However, previous evidences indicate that in the absence of YcgR, there is still an additional element that mediates motility impairment under high c-di-GMP levels. Here we have uncovered that cellulose per se is the factor that further promotes inhibition of bacterial motility once high c-di-GMP contents drive the activation of a sessile lifestyle. Inactivation of different genes of the bcsABZC operon, mutation of the conserved residues in the RxxxR motif of the BcsA PilZ domain, or degradation of the cellulose produced by BcsA rescued the motility defect of ?ycgR strains in which high c-di-GMP levels were reached through the overexpression of diguanylate cyclases. High c-di-GMP levels provoked cellulose accumulation around cells that impeded flagellar rotation, probably by means of steric hindrance, without affecting flagellum gene expression, exportation, or assembly. Our results highlight the relevance of cellulose in Salmonella lifestyle switching as an architectural element that is both essential for biofilm development and required, in collaboration with YcgR, for complete motility inhibition.

Zorraquino, Violeta; Garcia, Begona; Latasa, Cristina; Echeverz, Maite; Toledo-Arana, Alejandro; Valle, Jaione

2013-01-01

218

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.  

PubMed

Twenty-five years have passed since the discovery of cyclic dimeric (3'?5') GMP (cyclic di-GMP or c-di-GMP). From the relative obscurity of an allosteric activator of a bacterial cellulose synthase, c-di-GMP has emerged as one of the most common and important bacterial second messengers. Cyclic di-GMP has been shown to regulate biofilm formation, motility, virulence, the cell cycle, differentiation, and other processes. Most c-di-GMP-dependent signaling pathways control the ability of bacteria to interact with abiotic surfaces or with other bacterial and eukaryotic cells. Cyclic di-GMP plays key roles in lifestyle changes of many bacteria, including transition from the motile to the sessile state, which aids in the establishment of multicellular biofilm communities, and from the virulent state in acute infections to the less virulent but more resilient state characteristic of chronic infectious diseases. From a practical standpoint, modulating c-di-GMP signaling pathways in bacteria could represent a new way of controlling formation and dispersal of biofilms in medical and industrial settings. Cyclic di-GMP participates in interkingdom signaling. It is recognized by mammalian immune systems as a uniquely bacterial molecule and therefore is considered a promising vaccine adjuvant. The purpose of this review is not to overview the whole body of data in the burgeoning field of c-di-GMP-dependent signaling. Instead, we provide a historic perspective on the development of the field, emphasize common trends, and illustrate them with the best available examples. We also identify unresolved questions and highlight new directions in c-di-GMP research that will give us a deeper understanding of this truly universal bacterial second messenger. PMID:23471616

Römling, Ute; Galperin, Michael Y; Gomelsky, Mark

2013-03-01

219

Cyclic Di-GMP modulates the disease progression of Erwinia amylovora.  

PubMed

The second messenger cyclic di-GMP (c-di-GMP) is a nearly ubiquitous intracellular signal molecule known to regulate various cellular processes, including biofilm formation, motility, and virulence. The intracellular concentration of c-di-GMP is inversely governed by diguanylate cyclase (DGC) enzymes and phosphodiesterase (PDE) enzymes, which synthesize and degrade c-di-GMP, respectively. The role of c-di-GMP in the plant pathogen and causal agent of fire blight disease Erwinia amylovora has not been studied previously. Here we demonstrate that three of the five predicted DGC genes in E. amylovora (edc genes, for Erwinia diguanylate cyclase), edcA, edcC, and edcE, are active diguanylate cyclases. We show that c-di-GMP positively regulates the secretion of the main exopolysaccharide in E. amylovora, amylovoran, leading to increased biofilm formation, and negatively regulates flagellar swimming motility. Although amylovoran secretion and biofilm formation are important for the colonization of plant xylem tissues and the development of systemic infections, deletion of the two biofilm-promoting DGCs increased tissue necrosis in an immature-pear infection assay and an apple shoot infection model, suggesting that c-di-GMP negatively regulates virulence. In addition, c-di-GMP inhibited the expression of hrpA, a gene encoding the major structural component of the type III secretion pilus. Our results are the first to describe a role for c-di-GMP in E. amylovora and suggest that downregulation of motility and type III secretion by c-di-GMP during infection plays a key role in the coordination of pathogenesis. PMID:23475975

Edmunds, Adam C; Castiblanco, Luisa F; Sundin, George W; Waters, Christopher M

2013-03-08

220

Kajian Indeks Kualiti Air di Lembangan Sungai Paka, Terengganu (Water Quality Index Study in Paka River Basin, Terengganu)  

Microsoft Academic Search

ABSTRAK Suatu kajian mengenai Indeks Kualiti Air (IKA) telah dijalankan di lembangan Sungai Paka, Terengganu. Ianya melibatkan pengukuran oksigen terlarut, pH, permintaan oksigen biokimia, permintaan oksigen kimia, jumlah pepejal terampai dan ammonia di lapan buah stesen pensampelan. Hasil kajian menunjukkan semua stesen pensampelan berada dalam status bersih kecuali dua stesen tercemar yang terletak di Sungai Rengat dan Sungai Rasau. Walau

SUHAIMI SURATMAN; MOHAMAD AWANG; LOH AI LING; NORHAYATI MOHD TAHIR

221

Aging of di-isopropyl-phosphorylated human butyrylcholinesterase.  

PubMed Central

Organophosphate-inhibited cholinesterases can be reactivated by nucleophilic compounds. Sometimes phosphylated (phosphorylated or phosphonylated) cholinesterases become progressively refractory to reactivation; this can result from different reactions. The most frequent process, termed 'aging', involves the dealkylation of an alkoxy group on the phosphyl moiety through a carbocation mechanism. In attempting to determine the amino acid residues involved in the aging of butyrylcholinesterase (BuChE), the human BuChE gene was mutated at several positions corresponding to residues located at the rim of the active site gorge and in the vicinity of the active site. Mutant enzymes were expressed in Chinese hamster ovary cells. Wild-type BuChE and mutants were inhibited by di-isopropylfluorophosphate at pH 8.0 and 25 degrees C. Di-isopropyl-phosphorylated enzymes were incubated with the nucleophilic oxime 2-pyridine aldoxime methiodide and their reactivatability was determined. Reactivatability was expressed by the first-order rate constant of aging and/or the half-life of aging (t12). The t12 was found to be of the order of 60 min for wild-type BuChE. Mutations on Glu-197 increased t12 60-fold. Mutation W82A increased t12 13-fold. Mutation D70G increased t12 8-fold. Mutations in the vicinity of the active site serine residue had either moderate or no effect on aging; t12 was doubled for F329C and F329A, increased only 4-fold for the double mutant A328G+F329S, and no change was observed for the A328G mutant, indicating that the isopropoxy chain to be dealkylated does not directly interact with Ala-328 and Phe-329. These results were interpreted by molecular modelling of di-isopropylphosphorylated wild-type and mutant enzymes. Molecular dynamics simulations indicated that the isopropyl chain that is lost interacted with Trp-82, suggesting that Trp-82 has a role in stabilizing the carbonium ion that is released in the dealkylation step. This study emphasized the important role of the Glu-197 carboxylate in stabilizing the developing carbocation, and the allosteric control of the dealkylation reaction by Asp-70. Indeed, although Asp-70 does not interact with the phosphoryl moiety, mutation D70G affects the rate of aging. This indirect control was interpreted in terms of change in the conformational state of Trp-82 owing to internal motions of the Omega loop (Cys-65-Cys-92) in the mutant enzyme.

Masson, P; Fortier, P L; Albaret, C; Froment, M T; Bartels, C F; Lockridge, O

1997-01-01

222

Diffusion boriding of a press tool of DI22 steel  

SciTech Connect

For the purpose of increasing the quality and thickness uniformity of the borided case on parts of complex configuration they used a method of boriding in a molten active low-melting transport metal making it possible to combine diffusion impregnation with subsequent heat treatment. The transport metal was lithium. The final purpose of this investigation was the development of a method of hardening coatings for press tools of DI22 (4Kh4VMFS) steel with (%) 0.37-0.44 C, 0.2-0.5 Mn, 3.2-4.0 Cr, 0.8-1.2 W, 0.6-0.9 V, 1.2-1.5 Mo, and 0.6-1.0 Si.

Duryagina, Z.A.; Pastukhova, L.V.; Kitsak, M.I.

1987-11-01

223

Syntheses and structures of di(carboxylato)hexakis(acetonitrile)-dimolybdenum(II) bis(tetrafluoroborate) and trans-[di(?-acetato)-di(acetonitrile)di(?-bis(diphenylphosphino)amine)dimolybdenum(II)] bis(tetrafluoroborate)  

Microsoft Academic Search

Di(carboxylato)hexakis(acetonitrile)dimolybdenum(II)bis(tetrafluoroborate)s, [Mo2(?-O2CR)2(CH3CN)6](BF4)2 (R = CH3, CH2Cl, C(CH3)3, CF3) (3a-d), are easily accessible by reacting [Mo2(CH3CN)8](BF4)4 (2) with a stoichiometric amount or a slight excess of the appropriate carboxylic acid and anhydride in acetonitrile. A large excess of carboxylic acid leads to the formation of tetracarboxylates. As an example cis-[Mo2(?-O2CCH2Cl)2(CH3CN)6](BF4)2 (3b) has been structurally characterized. Crystallographic data are as follows: space

F. Albert Cotton; Fritz E. Kühn

1996-01-01

224

Molecular Structure of Di-t-butyl-peroxide  

NSDL National Science Digital Library

Di-tert-Butyl Peroxide is used as a polymerization initiator, curing agent and cross-linking agent. It is a highly reactive compound, which can be flammable when exposed to open flames and sparks. Upon exposure to heat, it undergoes gaseous decomposition. Its vapors are denser than air, hence it travels along the ground. It should be kept away from combustible material like wood, clothing, oil, and paper. This material may form flammable dust-air mixtures. Potential for a dust explosion may exist. Exposure to humans is mainly through inhalation, dermal contact. It can cause irritation to eye and skin, inhalation can cause cough, shortness of breath and sore throat, and ingestion can cause vomiting and abdominal cramps.

2002-09-27

225

Nanometer scale patterning using di-block copolymer  

NASA Astrophysics Data System (ADS)

Di-block copolymer thin films of PS-PI, PS-PB and PS-PMMA are investigated on Si substrates. The morphology evolution with polymer thickness is studied using optical microscopy. As-coated polymer exhibits a very smooth surface. After annealing polymer over glass-transition temperature, the polymer exhibits a smooth surface only at certain thickness L0. Transmission electron microscopy is used to study the microphase separation in polymer at different stages. Clear phase separation is observed in the polymer after staining with osmium tetraoxide (OsO4). Long time annealing increases the long-range ordering. After treatment with ozone, disappearance of dark dots due to staining of Os and appearance of white hole indicate that the polymer with double-bond is removed from copolymer film. Scanning electron microscopy shows that polymer films after reactive ion etching give a regular hole pattern which can serve as mask for nanometer scale patterning.

Zhao, Zuoming

2005-03-01

226

Overview of the recent performance of DI-BSCCO wire  

NASA Astrophysics Data System (ADS)

Sumitomo Electric has been developing the silver-sheathed Bi2223 multi-filamentary wires since the discovery of Bi-based superconductors. DI-BSCCO (Dynamically-Innovative BSCCO) is the high performance wires produced with the controlled-overpressure (CT-OP) sintering technique. The recent R&D activities have enabled to produce the 1000 m-long wires with Ic of 200 A and the maximum Ic reached 250 A at 77 K by short sample. Besides, the fine control of the carrier density with the non-stoichiometric oxygen enhanced the in-field Ic performances at lower temperature. At a magnetic field of 3 T applied normal to wire surface, the Ic of 280 A at 30 K, and 420 A at 20 K were successfully achieved. To meet the growing needs for various high temperature superconducting applications, Type HT wire with high mechanical strength and Type G wire with low thermal conductivity have been developed.

Nakashima, T.; Kobayashi, S.; Kagiyama, T.; Yamazaki, K.; Kikuchi, M.; Yamade, S.; Hayashi, K.; Sato, K.; Osabe, G.; Fujikami, J.

2012-12-01

227

Di-electrons from resonances in nucleon-nucleon collisions  

SciTech Connect

The contribution of the low-lying nucleon resonances P{sub 33}(1232), P{sub 11}(1440), D{sub 13}(1520), and S{sub 11}(1535) to the invariant-mass spectra of di-electrons stemming from the exclusive processes pp{yields}pp e{sup +}e{sup -} and pn{yields}pn e{sup +}e{sup -} is investigated within a fully covariant and gauge-invariant diagrammatical approach. We employ, within the one-boson exchange approximation, effective nucleon-meson interactions including the exchange mesons {pi}, {eta}, {sigma}, {omega}, and {rho} as well as excitations and radiative decays of the above low-lying nucleon resonances. The total contribution of these resonances is dominant; however, bremsstrahlung processes in pp and, in particular, pn collisions at beam energies of 1-2 GeV are still significant in certain phase-space regions.

Kaptari, L. P.; Kaempfer, B. [Forschungszentrum Dresden Rossendorf, PF 510119, D-01314 Dresden (Germany)

2009-12-15

228

Aqueous infrared carboxylate absorbances: Aliphatic di-acids  

USGS Publications Warehouse

Aqueous attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectra of 18 aliphatic di-carboxylic acids are reported as a function of pH. The spectra show isosbestic points and intensity changes which indicate that Beer's law is obeyed, and peak frequencies lie within previously reported ranges for aqueous carboxylates and pure carboxylic acids. Intensity sharing from the symmetric carboxylate stretch is evident in many cases, so that bands which are nominally due to alkyl groups show increased intensity at higher pH. The asymmetric stretch of the HA- species is linearly related to the microscopic acidity constant of the H2A species, with ??pK 2 intervening atoms). The results suggest that aqueous ATR-FTIR may be able to estimate 'intrinsic' pKa values of carboxylic acids, in addition to providing quantitative estimates of ionization. ?? 1998 Elsevier Science B.V. All rights reserved.

Cabaniss, S. E.; Leenheer, J. A.; McVey, I. F.

1998-01-01

229

Cytotoxic Activity and Apoptosis-Inducing Potential of Di-spiropyrrolidino and Di-spiropyrrolizidino Oxindole Andrographolide Derivatives  

PubMed Central

Anticancer role of andrographolide is well documented. To find novel potent derivatives with improved cytotoxicity than andrographolide on cancer cells, two series of di-spiropyrrolidino- and di-spiropyrrolizidino oxindole andrographolide derivatives prepared by cyclo-addition of azomethine ylide along with sarcosine or proline (viz. sarcosine and proline series respectively) and substitution of different functional groups (-CH3, -OCH3 and halogens) were examined for their cytotoxic effect on a panel of six human cancer cell lines (colorectal carcinoma HCT116 cells, pancreatic carcinoma MiaPaCa-2 cells, hepatocarcinoma HepG2 cells, cervical carcinoma HeLa cells, lung carcinoma A549 and melanoma A375 cells). Except halogen substituted derivatives of proline series (viz. CY2, CY14 and CY15 for Br, Cl and I substitution respectively), none of the other derivatives showed improved cytotoxicity than andrographolide in the cancer cell lines examined. Order of cytotoxicity of the potent compounds is CY2>CY14>CY15>andrographolide. Higher toxicity was observed in HCT116, MiaPaCa-2 and HepG2 cells. CY2, induced death of HCT116 (GI50 10.5), MiaPaCa-2 (GI50 11.2) and HepG2 (GI50 16.6) cells were associated with cell rounding, nuclear fragmentation and increased percentage of apoptotic cells, cell cycle arrest at G1 phase, ROS generation, and involvement of mitochondrial pathway. Upregulation of Bax, Bad, p53, caspases-3,-9 and cleaved PARP; downregulation of Bcl-2, cytosolic NF-?B p65, PI3K and p-Akt; translocation of P53/P21, NF-?B p65 were seen in CY2 treated HCT116 cells. Thus, three halogenated di-spiropyrrolizidino oxindole derivatives of andrographolide are found to be more cytotoxic than andrographolide in some cancer cells. The most potent derivative, CY2 induced death of the cancer cells involves ROS dependent mitochondrial pathway like andrographolide.

Hazra, Abhijit; Naskar, Subhendu; Nandy, Abhishek; Munda, Rudra Narayan; Das, Subhadip; Chatterjee, Nabanita; Mondal, Nirup Bikash; Banerjee, Sukdeb; Saha, Krishna Das

2013-01-01

230

Allosteric tertiary interactions pre-organize the c-di-GMP riboswitch and accelerate ligand binding  

PubMed Central

Cyclic diguanylate (c-di-GMP) is a bacterial second messenger important for physiologic adaptation and virulence. Class-I c-di-GMP riboswitches are phylogenetically widespread and thought to mediate pleiotropic genetic responses to the second messenger. Previous studies suggest that the RNA aptamer domain switches from an extended free state to a compact, c-di-GMP-bound conformation in which two helical stacks dock side-by-side. Single molecule fluorescence resonance energy transfer (smFRET) experiments reveal that the free RNA exists in four distinct populations that differ in dynamics in the extended and docked conformations. In the presence of c-di-GMP and Mg2+, a stably docked population (>30 min) becomes predominant. smFRET mutant analysis demonstrates that tertiary interactions distal to the c-di-GMP binding site strongly modulate the RNA population structure, even in the absence of c-di-GMP. These allosteric interactions accelerate ligand recognition by pre-organizing the RNA, favoring rapid c-di-GMP binding.

Wood, Sharla; Ferre-D'Amare, Adrian R.; Rueda, David

2012-01-01

231

Functional Characterization of Core Components of the Bacillus subtilis Cyclic-Di-GMP Signaling Pathway.  

PubMed

Bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) is an intracellular second messenger that regulates adaptation processes, including biofilm formation, motility, and virulence in Gram-negative bacteria. In this study, we have characterized the core components of a c-di-GMP signaling pathway in the model Gram-positive bacterium Bacillus subtilis. Specifically, we have directly identified and characterized three active diguanylate cyclases, DgcP, DgcK, and DgcW (formerly YtrP, YhcK, and YkoW, respectively), one active c-di-GMP phosphodiesterase, PdeH (formerly YuxH), and a cyclic-diguanylate (c-di-GMP) receptor, DgrA (formerly YpfA). Furthermore, elevation of c-di-GMP levels in B. subtilis led to inhibition of swarming motility, whereas biofilm formation was unaffected. Our work establishes paradigms for Gram-positive c-di-GMP signaling, and we have shown that the concise signaling system identified in B. subtilis serves as a powerful heterologous host for the study of c-di-GMP enzymes from bacteria predicted to possess larger, more-complex signaling systems. PMID:23893111

Gao, Xiaohui; Mukherjee, Sampriti; Matthews, Paige M; Hammad, Loubna A; Kearns, Daniel B; Dann, Charles E

2013-07-26

232

Identification of c-di-GMP derivatives resistant to an EAL domain phosphodiesterase.  

PubMed

The bacterial second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) controls important biological processes such as biofilm formation, virulence response, and motility. This second messenger is sensed by macromolecular targets inside the cell, both protein and RNA, which induce specific phenotypic responses critical for bacterial survival. One class of enzymes responsible for regulating the intracellular concentration of c-di-GMP, and therefore the physiological behavior of the cell, consists of the EAL domain phosphodiesterases, which degrade the second messenger to its linear form, pGpG. Here, we investigate how base and backbone modifications of c-di-GMP affect the rate of cyclic dinucleotide degradation by an EAL domain protein (CC3396 from Caulobacter crescentus). The doubly substituted thiophosphate analogue is highly resistant to hydrolysis by this metabolizing enzyme but can still bind c-di-GMP riboswitch targets. We used these findings to develop a novel ribosyl phosphate-modified derivative of c-di-GMP containing 2'-deoxy and methylphosphonate substitutions that is charge neutral and demonstrate that this analogue is also resistant to EAL domain-catalyzed degradation. This suggests a general strategy for designing c-di-GMP derivatives with increased enzymatic stability that also possess desirable properties for development as chemical probes of c-di-GMP signaling. PMID:23256840

Shanahan, Carly A; Gaffney, Barbara L; Jones, Roger A; Strobel, Scott A

2013-01-03

233

Allosteric tertiary interactions preorganize the c-di-GMP riboswitch and accelerate ligand binding.  

PubMed

Cyclic diguanylate (c-di-GMP) is a bacterial second messenger important for physiologic adaptation and virulence. Class-I c-di-GMP riboswitches are phylogenetically widespread and thought to mediate pleiotropic genetic responses to the second messenger. Previous studies suggest that the RNA aptamer domain switches from an extended free state to a compact, c-di-GMP-bound conformation in which two helical stacks dock side-by-side. Single molecule fluorescence resonance energy transfer (smFRET) experiments now reveal that the free RNA exists in four distinct populations that differ in dynamics in the extended and docked conformations. In the presence of c-di-GMP and Mg(2+), a stably docked population (>30 min) becomes predominant. smFRET mutant analysis demonstrates that tertiary interactions distal to the c-di-GMP binding site strongly modulate the RNA population structure, even in the absence of c-di-GMP. These allosteric interactions accelerate ligand recognition by preorganizing the RNA, favoring rapid c-di-GMP binding. PMID:22380737

Wood, Sharla; Ferré-D'Amaré, Adrian R; Rueda, David

2012-03-13

234

Sensing the messenger: The diverse ways that bacteria signal through c-di-GMP  

PubMed Central

An intracellular second messenger unique to bacteria, c-di-GMP, has gained appreciation as a key player in adaptation and virulence strategies, such as biofilm formation, persistence, and cytotoxicity. Diguanylate cyclases containing GGDEF domains and phosphodiesterases containing either EAL or HD-GYP domains have been identified as the enzymes controlling intracellular c-di-GMP levels, yet little is known regarding signal transmission and the sensory targets for this signaling molecule. Although limited in number, identified c-di-GMP receptors in bacteria are characterized by prominent diversity and multilevel impact. In addition, c-di-GMP has been shown to have immunomodulatory effects in mammals and several eukaryotic c-di-GMP sensors have been proposed. The structural biology of c-di-GMP receptors is a rapidly developing field of research, which holds promise for the development of novel therapeutics against bacterial infections. In this review, we highlight recent advances in identifying bacterial and eukaryotic c-di-GMP signaling mechanisms and emphasize the need for mechanistic structure–function studies on confirmed signaling targets.

Krasteva, Petya Violinova; Giglio, Krista Michelle; Sondermann, Holger

2012-01-01

235

DiI staining of fine branches of Bonghan ducts on surface of rat abdominal organs.  

PubMed

Novel thread-like structures and corpuscles, designated Bonghan ducts (BHDs) and corpuscles (BHCs), are known to form a system of networked channels. Here, we tested the effectiveness of a fluorescent carbocyanine dye, DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), in staining BHDs and BHCs. DiI solution was infused into a BHC on the surface of a rat abdominal organ at a steady rate and the resulting labeling of neighboring BHCs connected via BHDs was examined, as identified by the red fluorescence of DiI. BHDs diameters tapered away from BHCs and formed tree-like branches with fine arborizations embedded in the membranous tissues at their terminal parts. In the proximal parts, DiI fluorescence appeared as continuous lines within BHDs, but a large portion of BHDs remained unstained. In the distal parts of BHDs, discontinuous elongated DiI microparticles were identified along the sinuses within BHDs. The results showed that inner spaces within the BHDs allowed DiI to flow and that BHDs have tree-like branches and terminal arborizations. In conclusion, DiI can be used in visualizing BHDs fine structures. PMID:20633506

Lee, Byung-Cheon; Jhang, Seong-Uk; Choi, Jae-Hong; Lee, So-Yeong; Ryu, Pan-Dong; Soh, Kwang-Sup

2009-12-01

236

Systematic identification of conserved bacterial c-di-AMP receptor proteins.  

PubMed

Nucleotide signaling molecules are important messengers in key pathways that allow cellular responses to changing environments. Canonical secondary signaling molecules act through specific receptor proteins by direct binding to alter their activity. Cyclic diadenosine monophosphate (c-di-AMP) is an essential signaling molecule in bacteria that has only recently been discovered. Here we report on the identification of four Staphylococcus aureus c-di-AMP receptor proteins that are also widely distributed among other bacteria. Using an affinity pull-down assay we identified the potassium transporter-gating component KtrA as a c-di-AMP receptor protein, and it was further shown that this protein, together with c-di-AMP, enables S. aureus to grow in low potassium conditions. We defined the c-di-AMP binding activity within KtrA to the RCK_C (regulator of conductance of K(+)) domain. This domain is also found in a second S. aureus protein, a predicted cation/proton antiporter, CpaA, which as we show here also directly binds c-di-AMP. Because RCK_C domains are found in proteinaceous channels, transporters, and antiporters from all kingdoms of life, these findings have broad implications for the regulation of different pathways through nucleotide-dependent signaling. Using a genome-wide nucleotide protein interaction screen we further identified the histidine kinase protein KdpD that in many bacteria is also involved in the regulation of potassium transport and a PII-like signal transduction protein, which we renamed PstA, as c-di-AMP binding proteins. With the identification of these widely distributed c-di-AMP receptor proteins we link the c-di-AMP signaling network to a central metabolic process in bacteria. PMID:23671116

Corrigan, Rebecca M; Campeotto, Ivan; Jeganathan, Tharshika; Roelofs, Kevin G; Lee, Vincent T; Gründling, Angelika

2013-05-13

237

Integration of the Second Messenger c-di-GMP into the Chemotactic Signaling Pathway  

PubMed Central

ABSTRACT Elevated intracellular levels of the bacterial second messenger c-di-GMP are known to suppress motility and promote sessility. Bacterial chemotaxis guides motile cells in gradients of attractants and repellents over broad concentration ranges, thus allowing bacteria to quickly adapt to changes in their surroundings. Here, we describe a chemotaxis receptor that enhances, as opposed to suppresses, motility in response to temporary increases in intracellular c-di-GMP. Azospirillum brasilense’s preferred metabolism is adapted to microaerophily, and these motile cells quickly navigate to zones of low oxygen concentration by aerotaxis. We observed that changes in oxygen concentration result in rapid changes in intracellular c-di-GMP levels. The aerotaxis and chemotaxis receptor, Tlp1, binds c-di-GMP via its C-terminal PilZ domain and promotes persistent motility by increasing swimming velocity and decreasing swimming reversal frequency, which helps A. brasilense reach low-oxygen zones. If c-di-GMP levels remain high for extended periods, A. brasilense forms nonmotile clumps or biofilms on abiotic surfaces. These results suggest that association of increased c-di-GMP levels with sessility is correct on a long-term scale, while in the short-term c-di-GMP may actually promote, as opposed to suppress, motility. Our data suggest that sensing c-di-GMP by Tlp1 functions similar to methylation-based adaptation. Numerous chemotaxis receptors contain C-terminal PilZ domains or other sensory domains, suggesting that intracellular c-di-GMP as well as additional stimuli can be used to modulate adaptation of bacterial chemotaxis receptors.

Russell, Matthew H.; Bible, Amber N.; Fang, Xin; Gooding, Jessica R.; Campagna, Shawn R.; Gomelsky, Mark; Alexandre, Gladys

2013-01-01

238

Systematic identification of conserved bacterial c-di-AMP receptor proteins  

PubMed Central

Nucleotide signaling molecules are important messengers in key pathways that allow cellular responses to changing environments. Canonical secondary signaling molecules act through specific receptor proteins by direct binding to alter their activity. Cyclic diadenosine monophosphate (c-di-AMP) is an essential signaling molecule in bacteria that has only recently been discovered. Here we report on the identification of four Staphylococcus aureus c-di-AMP receptor proteins that are also widely distributed among other bacteria. Using an affinity pull-down assay we identified the potassium transporter-gating component KtrA as a c-di-AMP receptor protein, and it was further shown that this protein, together with c-di-AMP, enables S. aureus to grow in low potassium conditions. We defined the c-di-AMP binding activity within KtrA to the RCK_C (regulator of conductance of K+) domain. This domain is also found in a second S. aureus protein, a predicted cation/proton antiporter, CpaA, which as we show here also directly binds c-di-AMP. Because RCK_C domains are found in proteinaceous channels, transporters, and antiporters from all kingdoms of life, these findings have broad implications for the regulation of different pathways through nucleotide-dependent signaling. Using a genome-wide nucleotide protein interaction screen we further identified the histidine kinase protein KdpD that in many bacteria is also involved in the regulation of potassium transport and a PII-like signal transduction protein, which we renamed PstA, as c-di-AMP binding proteins. With the identification of these widely distributed c-di-AMP receptor proteins we link the c-di-AMP signaling network to a central metabolic process in bacteria.

Corrigan, Rebecca M.; Campeotto, Ivan; Jeganathan, Tharshika; Roelofs, Kevin G.; Lee, Vincent T.; Grundling, Angelika

2013-01-01

239

Intrafibrillar Mineral May be Absent in Dentinogenesis Imperfecta Type II (DI-II)  

SciTech Connect

High-resolution synchrotron radiation computed tomography (SRCT) and small angle x-ray scattering (SAXS) were performed on normal and dentinogenesis imperfecta type II (DI-II) teeth. Three normal and three DI-II human third molars were used in this study. The normal molars were unerupted and had intact enamel; donors were female and ranged in age from 18-21y. The DI-II specimens, which were also unerupted with intact enamel, came from a single female donor age 20y. SRCT showed that the mineral concentration was 33% lower on average in the DI-II dentin with respect to normal dentin. The SAXS spectra from normal dentin exhibited low-angle diffraction peaks at harmonics of 67.6 nm, consistent with nucleation and growth of the apatite phase within gaps in the collagen fibrils (intrafibrillar mineralization). In contrast, the low-angle peaks were almost nonexistent in the DI-II dentin. Crystallite thickness was independent of location in both DI-II and normal dentin, although the crystallites were significantly thicker in DI-II dentin (6.8 nm (s.d. = 0.5) vs 5.1 nm (s.d. = 0.6)). The shape factor of the crystallites, as determined by SAXS, showed a continuous progression in normal dentin from roughly one-dimensional (needle-like) near the pulp to two-dimensional (plate-like) near the dentin-enamel junction. The crystallites in DI-II dentin, on the other hand, remained needle-like throughout. The above observations are consistent with an absence of intrafibrillar mineral in DI-II dentin.

Pople, John A.

2001-03-29

240

Di-aqua-bis-(pyridine-2-carboxyl-ato-?2 N,O)zinc di-methyl-formamide hemisolvate  

PubMed Central

In the title compound, [Zn(C6H4NO2)2(H2O)2]·0.5C3H7NO, the ZnII ion is coordinated in a distorted octa­hedral N2O4 environment by two N,O-chelating pyridine-2-carboxyl­ate ligands and two cis water mol­ecules. The chelating pyridine-2-carboxyl­ate ligands create two five-membered Zn/N/C/C/O rings, which form a dihedral angle of 86.4?(2)°. In the crystal, O—H?O hydrogen bonds link the complex mol­ecules into a two-dimensional network parallel to (100). The di­methyl­formamide solvent mol­ecule is disordered about a twofold rotation axis.

Croitor, Lilia; Chisca, Diana; Coropceanu, Eduard B.; Fonari, Marina S.

2013-01-01

241

Pubblicato da HYPNOS in giugno 2006 The New Neuroscience School of Therapeutic Hypnosis, Psychotherapy, and Rehabilitation La Nuova Scuola Di Neuroscienze, Ipnosi Terapeutica, Psicoterapia e Riabilitazione  

Microsoft Academic Search

Attualmente le arti della salute stanno testimoniando un'infusione straordinaria di scoperte fondamentalmente nuove da parte delle neuroscienze e della genomica funzionale che hanno importanti implicazioni sulla nostra comprensione della condizione umana a livelli estremamente profondi. Questo articolo delinea quattro principi di base della Nuova Scuola Di Neuroscienze, Ipnosi Terapeutica, Psicoterapia e Riabilitazione con lo scopo di preparare una nuova generazione

Ernest L. Rossi; Salvatore Iannotti; Kathryn L. Rossi

242

A novel synthesis of (di)-benzazocinones via an endocyclic N-acyliminium ion cyclisation.  

PubMed

The triflic acid-mediated endocyclic N-acyliminium ion cyclisation provides a facile synthesis of (di)-benzazocinones. On reduction of the 10-phenyl derivative, an unusually non-polar tertiary alkylamine was obtained. PMID:21234463

King, Frank D; Aliev, Abil E; Caddick, Stephen; Tocher, D A

2011-01-13

243

Lysozyme-catalysed hydrolysis of some ?-aryl di-N-acetylchitobiosides  

PubMed Central

1. Four ?-aryl di-N-acetylchitobiosides and ?-S-phenyl di-N-acetylthiochitobioside have been prepared and shown to be substrates for hen's-egg-white lysozyme. 2. The lysozyme-catalysed hydrolysis of these substrates obeys Michaelis–Menten kinetics. 3. The Michaelis constants, Km, for the ?-aryl di-N-acetyl-chitobiosides are almost independent of the aglycone, whereas the catalytic constants, kcat., show a marked dependence, giving a Hammett reaction constant, ?, equal to 1·2; this suggests the rate-determining step involves concerted acid–base or acid–nucleophilic catalysis. 4. This conclusion is supported by the Michaelis–Menten constants found for ?-S-phenyl di-N-acetylthiochitobioside. 5. A three-step reaction pathway is proposed, and mechanisms are suggested that would account for the evidence currently available.

Lowe, G.; Sheppard, G.; Sinnott, M. L.; Williams, A.

1967-01-01

244

21 CFR 172.150 - 4-Hydroxymethyl-2,6-di-tert-butylphenol.  

Code of Federal Regulations, 2013 CFR

...AND HUMAN SERVICES (CONTINUED) FOOD FOR HUMAN CONSUMPTION (CONTINUED) FOOD ADDITIVES PERMITTED FOR DIRECT ADDITION TO FOOD FOR HUMAN CONSUMPTION Food Preservatives § 172.150 4-Hydroxymethyl-2,6-di-tert -butylphenol....

2013-04-01

245

Aerobic Biodegradation of DI-n-Butylphthalate by Pure and Mixed Bacterial Species.  

National Technical Information Service (NTIS)

Di-n-butylphthalate (DBP), an organic ester, is primarily used as a plasticizer during the preparation of explosive mixtures. It is abundantly found in the wastewater generated from DBP production plants and munitions manufacturing facilities and at conce...

B. W. Brodman C. Christodoulatos S. Bhaumik

1996-01-01

246

Il trattamento di una grave neuropatia diabetica con insulina somministrata per via endorachidea  

Microsoft Academic Search

Riassunto Viene descritto un caso di grave poliradicoloneurite diabetica che non aveva tratto alcun miglioramento da un lungo trattamento con vitamina B1 e B12 ad alti dosaggi. Esso giunse a guarigione somministrando insulina direttamente nello speco vertebrale.

Sergio Marigo; Enrico Ferrero

1964-01-01

247

The oxidation of 2,6-di-tert-butyl-4-methylphenol  

USGS Publications Warehouse

The products formed in the oxidation of 2,6-di-tert-butyl-4-methylphenol with oxygen and sodium hydroxide at about 100?? are 3,5-di-tert-butyl-4-hydroxybenzaldehyde, trimethylacetic acid, an acidic compound C14H22O3, and probably 2,6-di-tert-butylbenzoquinone (which was actually isolated in the similar oxidation of the above-named benzaldehyde), in addition to compounds previously reported. Some of the properties of C14H22O3 are given, and the oxidation of it to 2,3-di-tert-butylsuccinic anhydride is described, but assignment of structure is reserved pending the completion of more experimental work.

Yohe, G. R.; Dunbar, J. E.; Pedrotti, R. L.; Scheidt, F. M.; Lee, F. G. H.; Smith, E. C.

1956-01-01

248

Safety Assessment of Di(2-ethylhexyl)phthalate (DEHP) Released from PVC Medical Devices.  

National Technical Information Service (NTIS)

Patients undergoing medical procedures such as IV therapy, enteral and parenteral nutrition support, blood transfusion, hemodialysis and peritoneal dialysis, cardiopulmonary bypass (CPB) and extracorporeal membrane oxygenation (ECMO) can be exposed to di-...

2004-01-01

249

DECHLORINATION ACTIVITY (CROSS-ACCLIMATION) OF FRESHWATER SEDIMENTS ADAPTED TO MONO- AND DI-CHLOROPHENOLS  

EPA Science Inventory

The reductive dechlorination of chlorophenols (CPs) in sediment slurries (10% solids) adapted to dechlorinate mono- and di-CPs (DCP) was investigated to define the regiospecificity of the dechlorination reaction. nadapted sediment slurries amended with various ortho-substituted C...

250

INFRAGILIMENTO DA IDROGENO IN BOMBOLE IN ACCIAIO PER TRASPORTO GAS: STATO DELL'ARTE SULLE ATTUALI NORMATIVE E PRINCIPALI PROBLEMATICHE DI PROGETTAZIONE PER ACCIAI ALTO-RESISTENZIALI  

Microsoft Academic Search

Sommario L'impiego dell'idrogeno come veicolo di energia eco-compatibile per un'economia totalmente sostenibile richiede notevoli sforzi e sfide in settori che coinvolgono tutto il ciclo di vita dell'idrogeno, dalla produzione fino all'utilizzo finale. Particolari sforzi sono necessari nel campo dello stoccaggio, in quanto l'immagazzinamento di idrogeno gassoso necessita di pressioni molto più elevate di quelle tradizionalmente ed attualmente in uso con

M. Bellingardi; P. Bortot

251

Relating Flow Level Requirements to DiServ Packet Level Mechanisms1  

Microsoft Academic Search

We present a two level modeling approach for DiServ mechanisms. We rst sketch a flow model to capture the user requirements of dierentiation and to compare dierentiation to traditional best-eort TCP flow level results. We then study how the bandwidth allocations presented on the flow level can be modeled and achieved on the packet level. The DiServ mechanism we use

Eeva Nyberg; Samuli Aalto; Jorma Virtamo

2001-01-01

252

The use of social media tools in ViCaDiS Virtual Campus  

Microsoft Academic Search

This paper describes the results of piloting an online — mobile phone environment -ViCaDiS — Virtual Campus for Digital Students, as a co-operation between 6 universities from European Union to develop a common online learning environment enhanced with web 2.0 tools for supporting the online international co-operation at academic level. ViCaDiS facilitates a shift from Institutional Learning Environments towards Personal

Diana Andone; Radu Vasiu; Andrei Ternauciuc; Bogdan Dragulescu

2010-01-01

253

Lupus eritematoso sistemico: un caso di difficile diagnosi differenziale con forme infettive  

Microsoft Academic Search

purpurica, diffuse a tutto il corpo, compresi palmo delle mani e pianta dei piedi. È presente linfoadenopa- tia laterocervicale bilaterale, con linfoghiandole non dolenti, mobili, di 1-2 cm di diametro. Alla palpa- zione dell'addome si evidenzia epa- tosplenomegalia marcata con en- trambi gli organi debordanti tre dita dall'arcata costale. Gli esami ematici eseguiti all'in- gresso (Tab. I) mostrano aumento della

L. BIANCHI; S. BALLOTTI; F. G RISOLIA; S. C ASALINI; G. SALVI M. DI MARIA; G. C ALABRI; M. DE MARTINO

2001-01-01

254

Outcome valutativo in pazienti sottoposte a terapia con Ranelato di Stronzio: MOC QUS  

Microsoft Academic Search

Introduzione L'osteoporosi è una patologia a carico del sistema scheletrico che si caratterizza sia per una riduzione della massa ossea che per un deteriorarsi della microarchitettura trabecolare, responsabili dell'au- mento della fragilità ossea e quindi della maggiore predisposizione all'insorgenza di fratture. In Medicina Riabilitativa la possibilità di monitorare e gestire direttamente il trattamento per la cura dell'o- steoporosi rappresenta un

A. ROCCO; C. LJOKA

255

INDAGINE FEM PROBABILISTICA SULL'AFFIDABILITÀ DELLA TECNICA ELASTOGRAFICA PER IL RICONOSCIMENTO DI TUMORI  

Microsoft Academic Search

Sommario Tra gli strumenti a disposizione per la diagnosi precoce del tumore al seno la tecnica elastografica ad ultrasuoni vanta numerosi vantaggi, quali: la non-invasività della misura, l'assenza di effetti ionizzanti, l'ottima tollerabilità da parte dei pazienti, l'ampia diffusione delle apparecchiature ecografiche. Questa procedura diagnostica risulta però fortemente condizionata dalla presenza di molti fattori soggettivi. In questo studio sono stati

S. Celia; P. Forte; F. Faita

256

JaDiMa: Java Applications Distributed Management on Grid Platforms  

Microsoft Academic Search

This paper describes JaDiMa (Java Distributed Machine), a collaborative framework to construct high performance distributed Java applications on grid platforms. JaDiMa is a system that automatically manages remote libraries used in a Java application. It leverages on the advantages of portability, modularity, object oriented model and exi- bility of Java, while incorporating well known techniques of communi- cation and security.

Yudith Cardinale; Eduardo Blanco; Jesús De Oliveira

2006-01-01

257

Depositional and diagenetic history of the Alpe di Specie (Seelandalpe) fauna (Carnian, Northeastern Dolomites)  

Microsoft Academic Search

Summary  The fauna from Alpe di Specie (Seelandalpe) is one of the most important Carnian faunas in the world. Unfortunately it has\\u000a never been found in situ; it is only known from erratic boulders embedded in peaty soil in the meadows of the Alpe di Specie,\\u000a where they are distributed along a continuous beltabove the deep-water S. Cassiano Fm andbelow the

Franco Russo; Claudio Neri; Adelaide Mastandrea; Gianfranco Laghi

1991-01-01

258

Direct labeling and visualization of blood vessels with lipophilic carbocyanine dye DiI  

Microsoft Academic Search

We describe a protocol to rapidly and reliably visualize blood vessels in experimental animals. Blood vessels are directly labeled by cardiac perfusion using a specially formulated aqueous solution containing 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI), a lipophilic carbocyanine dye, which incorporates into endothelial cell membranes upon contact. By lateral diffusion, DiI also stains membrane structures, including angiogenic sprouts and pseudopodial processes that are

Yiwen Li; Ying Song; Lian Zhao; Gabriel Gaidosh; Alan M Laties; Rong Wen

2008-01-01

259

The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes  

Microsoft Academic Search

In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most other fluorochromes or radioisotopes, yet they are poorly soluble in

William Andrade; Timothy J. Seabrook; Miles G. Johnston; John B. Hay

1996-01-01

260

Effect of temperature and calcium on transneuronal diffusion of DiI in fixed brain preparations  

Microsoft Academic Search

The lipophilic tracer 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI) was used to label neuronal pathways in fixed goldfish brains. The normal procedure involving 4% paraformaldehyde as a fixative, applying DiI and storing the brain in the fixative at 40°C resulted in many cases in a rather diffuse labeling of fiber pathways and the occurrence of transneuronally labeled cells and fibers. We found that

Michael H Hofmann; Horst Bleckmann

1999-01-01

261

Efficacy of Quinoxaline2Carboxylate 1,4-Di-N-Oxide Derivatives in Experimental Tuberculosis  

Microsoft Academic Search

This study extends earlier reports regarding the in vitro efficacies of the 1,4-di-N-oxide quinoxaline deriv- atives against Mycobacterium tuberculosis and has led to the discovery of a derivative with in vivo efficacy in the mouse model of tuberculosis. Quinoxaline-2-carboxylate 1,4-di-N-oxide derivatives were tested in vitro against a broad panel of single-drug-resistant M. tuberculosis strains. The susceptibilities of these strains to

Esther Vicente; Raquel Villar; Asuncion Burguete; Beatriz Solano; Silvia Perez-Silanes; Ignacio Aldana; Joseph A. Maddry; Anne J. Lenaerts; Scott G. Franzblau; Sang-hyun Cho; Antonio Monge; Robert C. Goldman

2008-01-01

262

Group contributions to enthalpies of solvation in octan-1-ol and di- n-butyl ether  

Microsoft Academic Search

The standard enthalpies of solvation of selected n-alkanes (hexane, heptane, nonane, decane, dodecane, hexadecane) and aromatic hydrocarbons (benzene, toluene, ethylbenzene) in 1-octanol and di-n-butyl ether (DBE) have been determined at 298.15K from experimental heats of solution. The values obtained, together with the data collected from literature, form a dataset of 89 and 59 enthalpies of solvation in 1-octanol and di-n-butyl

Luca Bernazzani; Paolo Gianni; Vincenzo Mollica; Pietro Pizzolla

2004-01-01

263

Interaction of metabolites with R. rhodochrous during the biodegradation of di-ester plasticizers  

Microsoft Academic Search

The commonly used plasticizers di-ethylhexyl phthalate (DEHP) and di-ethylhexyl adipate (DEHA) are known to partially degrade in the presence of soil microorganisms, such as Rhodococcus rhodochrous, releasing persistent and toxic metabolites. The metabolites adipic acid and 2-ethylhexanol were both shown to inhibit growth of the degrading microbe. 2-Ethylhexanol enhanced the activity of ethanol dehydrogenase – an enzyme involved in its

Sandro Nalli; David G. Cooper; Jim A. Nicell

2006-01-01

264

Efficient hydrogenation of biomass-derived cyclic di-esters to 1,2-diols.  

PubMed

The unprecedented homogeneous hydrogenation of cyclic di-esters, in particular biomass-derived glycolide and lactide, to the corresponding 1,2-diols is catalyzed by Ru(II) PNN (1) and Ru(II) CNN (2) pincer complexes under mild hydrogen pressure and (in the case of 1) neutral conditions. No racemization was observed when a chiral di-ester was used. PMID:22038098

Balaraman, Ekambaram; Fogler, Eran; Milstein, David

2011-10-31

265

Recent R&D progress on DI-BSCCO wires with high critical current properties  

Microsoft Academic Search

Sumitomo Electric has been developing the silver-sheathed Bi2223 multi-filamentary wires since the discovery of Bi-based superconductors. DI-BSCCO is the high performance wires produced with the controlled-overpressure (CT-OP) sintering technique. The present commercial DI-BSCCO can provide the uniform high critical current, Ic, up to 180A over 2000m, and recently 200A were succeeded to obtain with the same kind of 1000m-long wires,

T. Nakashima; S. Kobayashi; T. Kagiyama; K. Yamazaki; M. Kikuchi; S. Yamade; K. Hayashi; K. Sato; J. Shimoyama; H. Kitaguchi; H. Kumakura

2011-01-01

266

Recent Progress in High Performance Ag-Sheathed Bi2223 Wire (DI-BSCCO®)  

Microsoft Academic Search

Sumitomo Electric has been developing the silver-sheathed Bi2223 multi-filamentary wires since the discovery of Bi-based superconductors. DI-BSCCO is the high performance wires produced using the controlled-overpressure (CT-OP) sintering technique. The present commercial DI-BSCCO can provide the uniform high critical current, Ic, up to 180 A with length over 2000 m, and recently 200 A were succeeded to be obtained by

T. Kagiyama; K. Yamazaki; M. Kikuchi; S. Yamade; T. Nakashima; S. Kobayashi; K. Hayashi; K. Sato; J. Shimoyama; M. Inoue; K. Higashikawa; T. Kiss; H. Kitaguchi; H. Kumakura

2011-01-01

267

Un'ipotesi di azienda faunistico-venatoria per le aree interne della Sicilia (Madonie)  

Microsoft Academic Search

Un'ipotesi di azienda faunistico-venatoria per le aree interne della Sicilia (Madonie) (di Alfonso Pagnesi) - ABSTRACT: In today’s society the issue of the environment and respect for nature is gaining in significance and awareness with consumers. The Common market codes, starting from 1990, regarding the question of food safety, together with the recent food scandals has highlighted the consumer need

Alfonso Pagnesi

2001-01-01

268

Synthesis and Characterization of C8 Analogs of c-di-GMP  

Microsoft Academic Search

We have synthesized five analogs of c-di-GMP with different substituents at the guanine C8 position, to study their effects on the metal-dependent polymorphism we had previously demonstrated for the parent compound. Of these, only the K salt of c-di-Br-GMP, 2, forms higher order complexes, predominantly two different syn octamolecular ones. Its Na salt, as well as both the K and

Elizabeth Veliath; Seho Kim; Barbara L. Gaffney; Roger A. Jones

2011-01-01

269

Sex-related diVerences in habitat selection in wintering American kestrels, Falco sparverius  

Microsoft Academic Search

The American kestrel, Falco sparverius, has sex-related diVerences in habitat use during the non-breeding season, with females occupying more open habitats than males. Two competing hypotheses have been proposed to explain this phenomenon: (1) males and females prefer diVerent habitats, and (2) males and females prefer similar habitats, but larger females exclude smaller males from preferred areas. This study experimentally

DANIEL R. ARDIA; KEITH L. BILDSTEIN

1997-01-01

270

ECONOMIA DELLA CONOSCENZA E RETI DI CITTÀ: CITTÀ CREATIVE NELL'ERA DELLA CONOSCENZA  

Microsoft Academic Search

All'inizio del XXI secolo, l'economia della conoscenza mette in rilievo l'importanza del sapere localizzato come fattore di produzione nel nuovo dintorno competitivo. Il sapere codificato mostra una tendenza alla diminuzione del costo, al tempo che il sapere specifico localizzato è un fattore intangibile e di difficile trasmissione che acquista importanza nella spiegazione del successo produttivo. In questo contesto, la città

Joan Trullén; Rafa Boix

2001-01-01

271

Spectrophotometric Determination of Uranium in Ores Using Di2-Pyridyl Ketone Hydrazone Derivatives  

Microsoft Academic Search

Two methods for the spectrophotometric determination of uranium (VI) are described. The methods are based on the formation of colored complexes for uranium (VI) with (1)di-2-pyridyl ketone nicotinoylhydrazone (DPKNH), and (2)di-2-pyridyl ketone thiophenoylhydrazone (DPKTH) in 50% (v\\/v) ethanolic solution. The complexes formed a uranium (VI): complexing reagent mole ratio of 1:2. Maximum absorbance is at 373 nm for U(VI)DPKNH, and

Maher Abu-Eid; Nidal A. Zatar; Mohammad A. Al-Nuri; Mustafa Khamis; Shukri Khalaf

1992-01-01

272

DiVusion of end-grafted chain-like molecules on surfaces  

Microsoft Academic Search

We have studied the diVusion of short end-grafted chain-like molecules on smooth surfaces using the fluctuating bond model and Monte Carlo simulations. In our simulations, one end of each chain is restricted to lie on the surface, where it can move in a diVusive manner. The other segments of the chains can fluctuate freely above the surface. We study the

T. Hjelt; T. Ala-Nissila

273

Sul problema del cambiamento di variabili nella teoria delle distribuzioni (II)  

Microsoft Academic Search

Riassunto  Si espongono nuove considerazioni relative al problema del cambiamento di variabili nella teoria delle distribuzioni, in aggiunta\\u000a a quelle svolte in un nostro precedente lavoro. Nel primo numero si tratterà il problema della derivazione delle distribuzioni\\u000a trasformate in analogia al teorema classico sulla derivazione delle funzioni composte. Nei numeri successivi si tratterà soprattutto\\u000a della estensione, al caso di più variabili,

S. Albertoni; M. Cugiani

1953-01-01

274

Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.  

PubMed

Cyclic di-GMP (c-di-GMP) is a second messenger that regulates diverse cellular processes in bacteria, including motility, biofilm formation, cell-cell signaling, and host colonization. Studies of c-di-GMP signaling have chiefly focused on Gram-negative bacteria. Here, we investigated c-di-GMP signaling in the Gram-positive bacterium Bacillus subtilis by constructing deletion mutations in genes predicted to be involved in the synthesis, breakdown, or response to the second messenger. We found that a putative c-di-GMP-degrading phosphodiesterase, YuxH, and a putative c-di-GMP receptor, YpfA, had strong influences on motility and that these effects depended on sequences similar to canonical EAL and RxxxR-D/NxSxxG motifs, respectively. Evidence indicates that YpfA inhibits motility by interacting with the flagellar motor protein MotA and that yuxH is under the negative control of the master regulator Spo0A?P. Based on these findings, we propose that YpfA inhibits motility in response to rising levels of c-di-GMP during entry into stationary phase due to the downregulation of yuxH by Spo0A?P. We also present evidence that YpfA has a mild influence on biofilm formation. In toto, our results demonstrate the existence of a functional c-di-GMP signaling system in B. subtilis that directly inhibits motility and directly or indirectly influences biofilm formation. PMID:22821967

Chen, Yun; Chai, Yunrong; Guo, Jian-hua; Losick, Richard

2012-07-20

275

STUDIO DEI FENOMENI SICCITOSI IN EMILIA-ROMAGNA CON IL MODELLO DI BILANCIO IDRICO CRITERIA  

Microsoft Academic Search

La partecipazione di ARPA-SIM al progetto Interreg SEDEMED ha riguardato, per la parte meteorologica, lo studio dei fenomeni siccitosi in Emilia-Romagna attraverso l'indice SPI (Standardized Precipitation Index) e la sua correlazione con la circolazione atmosferica a larga scala e con pattern di variabilità atmosferica, quali la NAO (North Atlantic Oscillation). In ambito agrometeorologico è stata invece studiata la siccità agricola,

276

Monitoraggio della Qualita deel'aria Ambiente nella Stazione di Rilevamento dell'Istituto Superiore di Sanita nel 1999 (Monitoring of Ambient Air Quality as Recorded by the Monitoring Station at the Istituto Superiore di Sanita in 1999).  

National Technical Information Service (NTIS)

The paper reports the processing and assessment of the results obtained from the determination of air pollutants during 1999 recorded by means of a monitoring station at the Istituto Superiore di Sanita. The station - which is not used to control air poll...

M. Ferdinandi M. Inlessis S. Puledda G. Viviano

2001-01-01

277

Additive property of secondary relaxation processes in di-n-octyl and di-isooctyl phthalates: signature of non-Johari-Goldstein relaxation.  

PubMed

Broadband dielectric spectroscopy was used to study relaxation dynamics of supercooled di-n-octyl phthalate, di-isooctyl phthalate, and their mixtures. Additionally, low temperature measurements were performed to investigate the nature of the secondary relaxation processes in both glass formers. The authors found that the secondary relaxation observed in the mixture is the additive sum of the secondary relaxations of the two components. This experimental evidence indicates that these secondary relaxation processes are intramolecular in origin, and they are non-Johari-Goldstein secondary relaxations. PMID:17492868

Kaminska, Ewa; Kaminski, Kamil; Paluch, Marian; Ziolo, Jerzy; Ngai, K L

2007-05-01

278

Structural Basis for c-di-GMP-Mediated Inside-Out Signaling Controlling Periplasmic Proteolysis  

PubMed Central

The bacterial second messenger bis-(3?–5?) cyclic dimeric guanosine monophosphate (c-di-GMP) has emerged as a central regulator for biofilm formation. Increased cellular c-di-GMP levels lead to stable cell attachment, which in Pseudomonas fluorescens requires the transmembrane receptor LapD. LapD exhibits a conserved and widely used modular architecture containing a HAMP domain and degenerate diguanylate cyclase and phosphodiesterase domains. c-di-GMP binding to the LapD degenerate phosphodiesterase domain is communicated via the HAMP relay to the periplasmic domain, triggering sequestration of the protease LapG, thus preventing cleavage of the surface adhesin LapA. Here, we elucidate the molecular mechanism of autoinhibition and activation of LapD based on structure–function analyses and crystal structures of the entire periplasmic domain and the intracellular signaling unit in two different states. In the absence of c-di-GMP, the intracellular module assumes an inactive conformation. Binding of c-di-GMP to the phosphodiesterase domain disrupts the inactive state, permitting the formation of a trans-subunit dimer interface between adjacent phosphodiesterase domains via interactions conserved in c-di-GMP-degrading enzymes. Efficient mechanical coupling of the conformational changes across the membrane is realized through an extensively domain-swapped, unique periplasmic fold. Our structural and functional analyses identified a conserved system for the regulation of periplasmic proteases in a wide variety of bacteria, including many free-living and pathogenic species.

Madden, Dean R.; O'Toole, George A.; Sondermann, Holger

2011-01-01

279

Identification of ligand analogues that control c-di-GMP riboswitches.  

PubMed

Riboswitches for the bacterial second messenger c-di-GMP control the expression of genes involved in numerous cellular processes such as virulence, competence, biofilm formation, and flagella synthesis. Therefore, the two known c-di-GMP riboswitch classes represent promising targets for developing novel modulators of bacterial physiology. Here, we examine the binding characteristics of circular and linear c-di-GMP analogues for representatives of both class I and II c-di-GMP riboswitches derived from the pathogenic bacterium Vibrio choleae (class I) and Clostridium difficile (class II). Some compounds exhibit values for apparent dissociation constant (K(D)) below 1 ?M and associate with riboswitch RNAs during transcription with a speed that is sufficient to influence riboswitch function. These findings are consistent with the published structural models for these riboswitches and suggest that large modifications at various positions on the ligand can be made to create novel compounds that target c-di-GMP riboswitches. Moreover, we demonstrate the potential of an engineered allosteric ribozyme for the rapid screening of chemical libraries for compounds that bind c-di-GMP riboswitches. PMID:22646696

Furukawa, Kazuhiro; Gu, Hongzhou; Sudarsan, Narasimhan; Hayakawa, Yoshihiro; Hyodo, Mamoru; Breaker, Ronald R

2012-06-19

280

c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.  

PubMed

The bacterium Bacillus subtilis produces the DNA integrity scanning protein (DisA), a checkpoint protein that delays sporulation in response to DNA damage. DisA scans the chromosome and pauses at sites of DNA lesions. Structural analysis showed that DisA synthesizes the small molecule cyclic diadenosine monophosphate (c-di-AMP). Here, we demonstrate that the intracellular concentration of c-di-AMP rises markedly at the onset of sporulation in a DisA-dependent manner. Furthermore, exposing sporulating cells to DNA-damaging agents leads to a global decrease in the level of this molecule. This drop was associated with stalled DisA complexes that halt c-di-AMP production and with increased levels of the c-di-AMP-degrading enzyme YybT. Reduced c-di-AMP levels cause a delay in sporulation that can be reversed by external supplementation of the molecule. Thus, c-di-AMP acts as a secondary messenger, coupling DNA integrity with progression of sporulation. PMID:21566650

Oppenheimer-Shaanan, Yaara; Wexselblatt, Ezequiel; Katzhendler, Jehoshua; Yavin, Eylon; Ben-Yehuda, Sigal

2011-05-13

281

Di-trinucleon cluster resonances in A=6 isobar nuclei  

SciTech Connect

The di-trinucleon clustering resonances of {sup 3}He+{sup 3}He in {sup 6}Be and t+t in {sup 6}He were studied via the {sup 6}Li({sup 3}He,t {sup 3}He) and {sup 6}Li({sup 7}Li,{sup 7}Be t) reactions at incident energies of 450 and 455 MeV, respectively. A new resonance in {sup 6}Be was found at E{sub x}=18.0{+-}1.2 MeV with a width of 9.2{+-}1.0 MeV in the binary decay channel of {sup 6}Be{yields}{sup 3}He+{sup 3}He. The resonance in {sup 6}He previously reported was also observed at E{sub x}=18.0{+-}1.0 MeV with a width of 9.5{+-}1.0 MeV in the binary decay channel of {sup 6}He {yields}t+t. The branching ratios of the resonances for each binary decay were both 0.7{+-}1.0, which was larger than the branching ratio calculated in a statistical model by two orders of magnitude. The angular correlations measured for t and {sup 3}He particles in the binary decay show a contribution dominantly from the multipolarity of L=1. We suggest that the resonances are the members of the isobaric triplet of the {sup 3}P resonances in A=6 nuclei.

Yamagata, T.; Akimune, H.; Hara, K.Y.; Kinoshita, M.; Ohta, M.; Shiokawa, A.; Utsunomiya, H. [Department of Physics, Konan University, Kobe 658-8501 (Japan); Nakayama, S.; Fushimi, K.; Ichihara, K.; Matsui, Y. [Department of Physics, University of Tokushima, Tokushima 770-8502 (Japan); Fujiwara, M. [Research Center for Nuclear Physics, Osaka University, Osaka 567-0047 (Japan); Advance Photon Research Center, JAERI, Kizu 619-0215 (Japan); Greenfield, M.B. [Department of Physics, International Christian University, Tokyo 113-0033 (Japan); Hara, K.; Hashimoto, H.; Kawase, K.; Nakanishi, K. [Research Center for Nuclear Physics, Osaka University, Osaka 567-0047 (Japan); Tanaka, M. [Kobe Tokiwa College, Kobe 654-0838 (Japan); Yosoi, M. [Department of Physics, Kyoto University, Kyoto 606-8502 (Japan)

2005-06-01

282

Dermal oncogenicity bioassays of di-sec-butoxyacetophenone and diethoxyacetophenone.  

PubMed

Groups of 40 male C3H/HeJ mice were treated 3 times per week for their lifetime on the skin of the back with undiluted di-sec-butoxyacetophenone (DBAP), or with diethoxyacetophenone (DEAP), undiluted and as a 50% dilution in acetone. Approximate doses per application were 22.4 mg for DBAP, and 25.0 or 11.3 mg for DEAP. Two negative control groups received acetone only. Both DBAP and DEAP (undiluted) had weak tumorigenic activity. In the DBAP group, one mouse developed a squamous-cell papilloma, whereas one mouse in the high-dose DEAP group had a squamous-cell carcinoma, both tumors appearing in the treatment area. No skin tumors were observed in the other groups. Both DEAP-treated groups also had substantial incidences of hyperkeratosis, epidermal hyperplasia, and dermatitis. No significant reduction in survival was observed in the test groups. The occurrence of the skin tumors in the DBAP- and DEAP-treated groups is considered to be treatment-related and suggestive of oncogenic potential, because of the extremely low historical control incidence of skin tumors in similar studies. PMID:6492197

DePass, L R; Fowler, E H; Weil, C S

1984-01-01

283

A purple acidophilic di-ferric DNA ligase from Ferroplasma  

PubMed Central

We describe here an extraordinary purple-colored DNA ligase, LigFa, from the acidophilic ferrous iron-oxidizing archaeon Ferroplasma acidiphilum, a di-ferric enzyme with an extremely low pH activity optimum. Unlike any other DNA ligase studied to date, LigFa contains two Fe3+-tyrosinate centers and lacks any requirement for either Mg2+ or K+ for activity. DNA ligases from closest phylogenetic and ecophysiological relatives have normal pH optima (6.0–7.5), lack iron, and require Mg2+/K+ for activity. Ferric iron retention is pH-dependent, with release resulting in partial protein unfolding and loss of activity. Reduction of the Fe3+ to Fe2+ results in an 80% decrease in DNA substrate binding and an increase in the pH activity optimum to 5.0. DNA binding induces significant conformational change around the iron site(s), suggesting that the ferric irons of LigFa act both as structure organizing and stabilizing elements and as Lewis acids facilitating DNA binding at low pH.

Ferrer, Manuel; Golyshina, Olga V.; Beloqui, Ana; Bottger, Lars H.; Andreu, Jose M.; Polaina, Julio; De Lacey, Antonio L.; Trautwein, Alfred X.; Timmis, Kenneth N.; Golyshin, Peter N.

2008-01-01

284

Francium sources at Laboratori Nazionali di Legnaro: Design and performance  

SciTech Connect

A facility for the production of radioactive francium is operating at the laboratories of the Istituto Nazionale di Fisica Nucleare (INFN) in Legnaro, Italy. The goal is to collect a cold sample of radioactive atoms in a magneto-optical trap for studies in atomic, nuclear, and particle physics. Production of francium is achieved via the fusion-evaporation reaction {sup 197}Au({sup 18}O,kn){sup 215-k}Fr generated by a {approx}100-MeV {sup 18}O{sup 6+} beam on a thick gold target. The production target is heated to {approx}1200 K and kept at a potential of +3 kV to enhance Fr diffusion and surface desorption. Average production rates are 0.7x10{sup 6} ions/s for {sup 210}Fr with a primary beam flux of 10{sup 12} particles/s, with peaks of 2x10{sup 6} ions/s. Details are given on the design and construction of the production targets and on the measurements that characterize their performance.

Stancari, G.; Veronesi, S.; Corradi, L.; Atutov, S.N.; Calabrese, R.; Dainelli, A.; Mariotti, E.; Moi, L.; Sanguinetti, S.; Tomassetti, L. [Dipartimento di Fisica and INFN, 44100 Ferrara (Italy); Dipartimento di Fisica and INFN, 53100 Siena (Italy); INFN Laboratori Nazionali di Legnaro, 35020 Legnaro PD (Italy); Dipartimento di Fisica and INFN, 44100 Ferrara (Italy); INFN Laboratori Nazionali di Legnaro, 35020 Legnaro PD (Italy); Dipartimento di Fisica and INFN, 53100 Siena (Italy); Dipartimento di Fisica and INFN, 56127 Pisa (Italy); Dipartimento di Fisica and INFN, 44100 Ferrara (Italy)

2006-03-15

285

Sensitive and fast mapping of di-base encoded reads  

PubMed Central

Motivation: Discovering variation among high-throughput sequenced genomes relies on efficient and effective mapping of sequence reads. The speed, sensitivity and accuracy of read mapping are crucial to determining the full spectrum of single nucleotide variants (SNVs) as well as structural variants (SVs) in the donor genomes analyzed. Results: We present drFAST, a read mapper designed for di-base encoded ‘color-space’ sequences generated with the AB SOLiD platform. drFAST is specially designed for better delineation of structural variants, including segmental duplications, and is able to return all possible map locations and underlying sequence variation of short reads within a user-specified distance threshold. We show that drFAST is more sensitive in comparison to all commonly used aligners such as Bowtie, BFAST and SHRiMP. drFAST is also faster than both BFAST and SHRiMP and achieves a mapping speed comparable to Bowtie. Availability: The source code for drFAST is available at http://drfast.sourceforge.net Contact: calkan@u.washington.edu

Hormozdiari, Farhad; Hach, Faraz; Sahinalp, S. Cenk; Eichler, Evan E.; Alkan, Can

2011-01-01

286

Forward di-hadron correlation study at STAR  

NASA Astrophysics Data System (ADS)

Quarks and Gluons, known generically as partons, are the current known fundamental constituents of the proton. The gluon density increases as the longitudinal momentum faction (x) of the gluon decreases, but it cannot increase without bound and saturation is expected at a certain low x value. The dynamics of quarks and gluons at low x is an open question. Studies of correlations between pairs of particles emitted in the forward direction at STAR aim to probe the small-x gluons in protons and in denser nuclear targets like gold. Suppression of back-to-back forward 0? - forward 0? azimuthal correlations which is consistent with Color Glass Condensate (CGC) prediction has been observed in central d+Au collisions at STAR. By studying the rapidity dependence of the forward triggered di-hadron correlations in p+p and d+Au collisions at ?s=200GeV, we aim to address the question of how sharp the transition is in going from a dilute parton gas to the dense parton state. STAR has nearly continuous electromagnetic calorimeter coverage in pseudo-rapidity -1

Li, Xuan

2012-10-01

287

Biotransformation of di(2-ethylhexyl)phthalate by rainbow trout  

SciTech Connect

The biotransformation of di(2-ethylhexyl)phthalate (DEHP) was studied in rainbow trout (Oncorhynchus mykiss) following intravascular administration. Methyl-esterified metabolites were identified using rodent-derived standards and non-linear gradient elution HPLC; metabolites were confirmed by gas chromatography. Similarities between the biotransformation of DEHP by rainbow trout and mammalian species included (a) mono-ethylhexyl phthalate (MEHP) appeared to be the obligatory first step in DEHP metabolism; (b) the phthalate ring was not oxidized; (c) phthalic acid was a minor metabolite; and (d) several metabolites contained multiple oxidations of the 2-ethylhexyl moiety of MEHP. No metabolites unique to rainbow trout were identified. However, fewer oxidized metabolites were identified in rainbow trout than in mammalian species, possibly due to limited mitochondrial metabolism of MEHP in rainbow trout. The amount of biliary MEHP glucuronide after intravascular administration of DEHP was substantially less than reported in rainbow trout exposed to DEHP via the water. Results confirmed that DEHP metabolism in rainbow trout proceeds by initial rapid formation of MEHP, followed by excretion or extensive oxidation by microsomal P450.

Barron, M.G. [RCG/Hagler Bailly, Inc., Boulder, CO (United States); Albro, P.W. [National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States); Hayton, W.L. [Ohio State Univ., Columbus, OH (United States). College of Pharmacy

1995-05-01

288

Di-electron and two-photon widths in charmonium  

SciTech Connect

The vector and pseudoscalar decay constants are calculated in the framework of the Field Correlator Method. Di-electron widths: {Gamma}{sub ee}(J/{psi}) = 5.41 keV, {Gamma}{sub ee}({psi}'(3686)) = 2.47 keV, {Gamma}{sub ee}({psi}''(3770)) = 0.248 keV, in good agreement with experiment, are obtained with the same coupling, {alpha}{sub s} = 0.165, in the QCD radiative corrections. We show that the larger {alpha}{sub s} = 0.191 {+-} 0.004 is needed to reach agreement with experiment for {Gamma}{sub {gamma}{gamma}}({chi}c) = 7.22 keV, {Gamma}{sub {gamma}{gamma}}({chi}c({sup 3}P0)) = 3.3 keV, {Gamma}{sub {gamma}{gamma}}{chi}c({sup 3}P{sub 2}) = 0.54 keV, and also for {Gamma}(J/{psi} {yields} 3g) = 59.5 keV, {Gamma}(J/{psi} {yields} {gamma}2g) = 5.7 keV. Meanwhile, even larger {alpha}{sub s} = 0.238 gives rise to good description of {Gamma}({psi}' {yields} 3g) = 52.7 keV, {Gamma}({psi}' {yields} {gamma} 2g) = 3.5 keV, and provides correct ratio of the branching fractions: {Beta} (J/{psi} {yields} light hadrons)/{Beta} ({psi}' {yields} light hadrons) = 0.24.

Badalian, A. M., E-mail: badalian@itep.r [Institute of Theoretical and Experimental Physics (Russian Federation); Danilkin, I. V. [Moscow Engineering Physics Institute (Russian Federation)

2009-07-15

289

Absence of genomic imprinting at the DiGeorge locus  

SciTech Connect

In situ hybridization with fluorescence probes (FISH) on interphase nuclei allows evaluation of the stage of DNA replication. For example, in a diploid cell in G1, unreplicated DNA gives two single dots of hybridization whereas in a diploid cell in G2, for loci which have already replicated, the hybridization signal is seen as two pairs of doublets. In contrast, sequences which have an asynchronous replication are characterized by one double hybridization signal and one single hybridization signal. It has been shown recently that sequences subject to genomic imprinting have an asynchronous replication, i.e., the two homologous alleles have a different pattern of replication. We have tested the replication pattern of different sequences of the DiGeorge critical region using FISH. The results obtained with probes 48F8, C350, C237 and COS40 show no evidence of asynchronous replication. This suggests that these loci are not subject to imprinting. These results are in agreement with recent observation of cases of uniparental disomy of chromosome 22 without phenotypic features. Further studies are necessary to exclude other regions of chromosome 22 which might be subject to genomic imprinting.

Theophile, D.; Berube, D.; Auge, J.; Vekemans, M. [Hopital Necker-Enfants Malades, Paris (France)

1994-09-01

290

Progetto per l'Istituzione del Registro Nazionale degli Interventi di Protesti di Anca. (Design of the Italian National Hip Arthroplasty Registry).  

National Technical Information Service (NTIS)

Since 2002, the Istituto Superiore di Sanita (ISS, the Italian National Institute of Health) has been involved in studies relating to the evaluation of hip arthroplasty outcomes and to the setting up of implants registers. In particular, the ISS has been ...

M. Torre

2004-01-01

291

Dinamica di Specie Algali Tossiche nei Laghi di Albano e Nemi (Toxic Algal Species Dynamic in the Lakes of Albano and Nemi).  

National Technical Information Service (NTIS)

The results of a 27 month study (January 2001- April 2003) carried out by the Istituto Superiore di Sanith (the Italian National Institute of Health) have pointed out the presence of two steady populations of the toxic Cyanophycea Planktothrix rubescens i...

M. Bruno V. Messineo D. Mattei S. Melchiorre

2005-01-01

292

Structural and Biochemical Determinants of Ligand Binding by the c-di-GMP Riboswitch  

SciTech Connect

The bacterial second messenger c-di-GMP is used in many species to control essential processes that allow the organism to adapt to its environment. The c-di-GMP riboswitch (GEMM) is an important downstream target in this signaling pathway and alters gene expression in response to changing concentrations of c-di-GMP. The riboswitch selectively recognizes its second messenger ligand primarily through contacts with two critical nucleotides. However, these two nucleotides are not the most highly conserved residues within the riboswitch sequence. Instead, nucleotides that stack with c-di-GMP and that form tertiary RNA contacts are the most invariant. Biochemical and structural evidence reveals that the most common natural variants are able to make alternative pairing interactions with both guanine bases of the ligand. Additionally, a high-resolution (2.3 {angstrom}) crystal structure of the native complex reveals that a single metal coordinates the c-di-GMP backbone. Evidence is also provided that after transcription of the first nucleotide on the 3{prime}-side of the P1 helix, which is predicted to be the molecular switch, the aptamer is functional for ligand binding. Although large energetic effects occur when several residues in the RNA are altered, mutations at the most conserved positions, rather than at positions that base pair with c-di-GMP, have the most detrimental effects on binding. Many mutants retain sufficient c-di-GMP affinity for the RNA to remain biologically relevant, which suggests that this motif is quite resilient to mutation.

Smith, K.; Lipchock, S; Livingston,; Shanahan, C; Strobel, S

2010-01-01

293

Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly.  

PubMed

The dengue virus (DV) envelope (E) protein is important in mediating viral entry and assembly of progeny virus during cellular infection. Domains I and III (DI and DIII, respectively) of the DV E protein are connected by a highly conserved but poorly ordered region, the DI/DIII linker. Although the flexibility of the DI/DIII linker is thought to be important for accommodating the structural rearrangements undergone by the E protein during viral entry, the function of the linker in the DV infectious cycle is not well understood. In this study, we performed site-directed mutagenesis on conserved residues in the DI/DIII linker of the DV2 E protein and showed that the resulting mutations had little or no effect on the entry process but greatly affected virus assembly. Biochemical fractionation and immunofluorescence microscopy experiments performed on infectious virus as well as in a virus-like particle (VLP) system indicate that the DI/DIII linker mutants express the DV structural proteins at the sites of particle assembly near the ER but fail to form infectious particles. This defect is not due to disruption of E's interaction with prM and pr in immature and mature virions, respectively. Serial passaging of the DV2 mutant E-Y299F led to the identification of a mutation in the membrane-proximal stem region of E that fully compensates for the assembly defect of this DI/DIII linker mutant. Together, our results suggest a critical and previously unidentified role for the E protein DI/DIII linker region during the DV2 assembly process. PMID:22532681

de Wispelaere, Melissanne; Yang, Priscilla L

2012-04-24

294

Structural and biochemical determinants of ligand binding by the c-di-GMP riboswitch .  

PubMed

The bacterial second messenger c-di-GMP is used in many species to control essential processes that allow the organism to adapt to its environment. The c-di-GMP riboswitch (GEMM) is an important downstream target in this signaling pathway and alters gene expression in response to changing concentrations of c-di-GMP. The riboswitch selectively recognizes its second messenger ligand primarily through contacts with two critical nucleotides. However, these two nucleotides are not the most highly conserved residues within the riboswitch sequence. Instead, nucleotides that stack with c-di-GMP and that form tertiary RNA contacts are the most invariant. Biochemical and structural evidence reveals that the most common natural variants are able to make alternative pairing interactions with both guanine bases of the ligand. Additionally, a high-resolution (2.3 A) crystal structure of the native complex reveals that a single metal coordinates the c-di-GMP backbone. Evidence is also provided that after transcription of the first nucleotide on the 3'-side of the P1 helix, which is predicted to be the molecular switch, the aptamer is functional for ligand binding. Although large energetic effects occur when several residues in the RNA are altered, mutations at the most conserved positions, rather than at positions that base pair with c-di-GMP, have the most detrimental effects on binding. Many mutants retain sufficient c-di-GMP affinity for the RNA to remain biologically relevant, which suggests that this motif is quite resilient to mutation. PMID:20690679

Smith, Kathryn D; Lipchock, Sarah V; Livingston, Alison L; Shanahan, Carly A; Strobel, Scott A

2010-08-31

295

Electron microscopic differentiation of directly and transneuronally transported DiI and applications for studies of synaptogenesis  

Microsoft Academic Search

The neuronal tracer DiI is a lipophilic dye which diffuses along the lipid bilayer of membranes and sometimes will move transcellularly. We used this tracer to study the development of olivocochlear synapses in the auditory system in rats and chickens by applying DiI directly to severed axons in the olivocochlear bundle. Observations with epi-fluorescent microscopy showed that DiI had labelled

L. L Bruce; M. A Christensen; B Fritzsch

1997-01-01

296

Electron Microscopic Visualization of Receptor-mediated Endocytosis of DiI-labeled Lipoproteins by Diaminobenzidine Photoconversion  

Microsoft Academic Search

SUMMARY We present a modified diaminobenzidine (DAB) photoconversion method that enables staining of internalized DiI-labeled lipoproteins without the apparent punc- tate background staining that was observed with the original DAB photoconversion method. This is illustrated by the localization of DiI-labeled insect lipoproteins in natural re- cipient cells that internalize these lipoproteins by receptor-mediated endocytosis. Exposure to DiI-excitation light of cells

Nico P. Dantuma; Marian A. P. Pijnenburg; Jacques H. B. Diederen; Dick J. Van der Horst

1998-01-01

297

Penilaian Indeks Kualiti Air di Lembangan Sungai Ibai, Terengganu (Determination of Water Quality Index at Ibai River Basin, Terengganu)  

Microsoft Academic Search

ABSTRAK Penentuan kualiti air berdasarkan Indeks Kualiti Air-Jabatan Alam Sekitar (IKA) telah dilakukan di lembangan Sungai Ibai, Terengganu dari bulan Ogos hingga Disember 2003. Pensampelan telah dilakukan sebanyak empat kali di enam stesen pensampelan. Parameter-parameter yang diukur ialah pH, oksigen terlarut, permintaan oksigen biokimia, permintaan oksigen kimia, jumlah pepejal terampai dan ammonia. Keputusan daripada kajian mendapati purata nilai IKA di

SUHAIMI SURATMAN; ASMADI ALI; LO TING TING

298

Biomonitoring equivalents for di-isononyl phthalate (DINP).  

PubMed

Biomonitoring Equivalents (BEs) are defined as the concentration or range of concentrations of a chemical or its metabolite in a biological medium (blood, urine, or other medium) that is consistent with an existing health-based exposure guideline such as a reference dose (RfD) or tolerable daily intake (TDI). BE values can be used as a screening tool for the evaluation of population-based biomonitoring data in the context of existing risk assessments. This study reviews available health based risk assessments and exposure guidance values for di-isononyl phthalate (DINP) from Health Canada, the United States Consumer Product Safety Commission (US CPSC), and the European Food Safety Authority (EFSA). Controlled dosing data reporting the urinary excretion fractions of major DINP metabolites following administration of labeled DINP are reviewed, and BE values corresponding to the available exposure guidance values are derived assuming chronic, steady-state intake and excretion at those exposure values. The BE values range from 1500 to 3600?g/L (1900-4600?g/g creatinine) based on the sum of three oxidative metabolites. Sources of uncertainty relating to both the basis for the BE values and their use in evaluation of biomonitoring data, including the transience of the biomarkers relative to exposure frequency, are discussed. The BE values derived here can be used as screening tools for evaluation of population biomonitoring data for DINP in the context of existing risk assessments and can assist in prioritization of the potential need for additional risk assessment efforts for DINP relative to other chemicals. PMID:21466828

Hays, Sean M; Aylward, Lesa L; Kirman, Christopher R; Krishnan, Kannan; Nong, Andy

2011-04-03

299

DiMES divertor erosion experiments on DIII-D  

SciTech Connect

The DiMES (Divertor Material Evaluation Studies) mechanism allows insertion of material samples to the lower divertor floor of the DIII-D tokamak. The main purpose of these studies is to measure erosion rates and redeposition mechanisms under tokamak divertor plasma conditions in order to obtain a physical understanding of the erosion/redeposition processes and to determine its implications for fusion power plant plasma facing components. Thin metal films of Be, W, V, and Mo, were deposited on a Si depth-marked graphite sample and exposed to the steady-state outer strike point on DIII-D. A variety of surface analysis techniques are used to determine the erosion/redeposition of the metals and the carbon after 5--15 seconds of exposure. These short exposure times ensure controlled exposure conditions and the extensive array of DIII-D divertor diagnostics provide a well characterized plasma for modeling efforts. Erosion rates and redeposition lengths are found to decrease with the atomic number of the metallic species, as expected. Under these conditions, the peak net erosion rate for carbon is {approximately} 4 nm/s, with the erosion following the ion flux profile. Comparisons of the measured carbon erosion with REDEP code calculations show good agreement for both the absolute net erosion rate and its spatial variation. Measured erosion rates of the metals are smaller than predicted for sputtering from a bare metal surface, apparently due to effects of carbon deposition on the metal surface. Visible spectroscopic measurements of singly ionized Be have determined that the erosion process reaches steady-state during the exposure.

Whyte, D.G. [Institut National de la Recherche Scientifique, Varennes, PQ (Canada); Brooks, J.N. [Argonne National Lab., IL (United States); Wong, C.P.C.; West, W.P. [General Atomics, San Diego, CA (United States); Bastasz, R.; Wampler, W.R. [Sandia National Labs., Albuquerque, NM (United States); Rubinstein, J. [Illinois Univ., Urbana, IL (United States)

1996-06-01

300

Stereoelectronic effects on (di)terpene synthase product outcome†‡  

PubMed Central

Terpene synthases catalyze complex reactions, often forming multiple chiral centers in cyclized olefin products from acyclic allylic diphosphate precursors, yet have been suggested to exert little control over the actual reaction, instead largely serving as inert templates. However, recent results highlight stereoelectronic effects exerted by these enzymes. Perhaps not surprisingly, the pyrophosphate co-product released in the initiating and rate-limiting chemical step provides an obvious counter-ion that may drive carbocation migration towards itself. This is emphasized by the striking effects of a recently uncovered single residue switch for diterpene synthase product outcome, whereby substitution of hydroxyl residues for particular aliphatic residues has been shown to be sufficient to “short-circuit” complex cyclization and/or rearrangement reactions, with the converse change further found to be sufficient to increase reaction complexity. The mechanistic hypothesis for the observed effects is hydroxyl dipole stabilization of the specific carbocation formed by initial cyclization, enabling deprotonation of this early intermediate, whereas the lack of such stabilization (i.e. with an aliphatic side chain) leads to carbocation migration towards the pyrophosphate co-product, resulting in a more complex reaction. This is further consistent with the greater synergy exhibited between pyrophosphate and aza-analogs of late, relative to early, stage carbocation intermediates, and crystallographic analysis of the monoterpene cyclase bornyl diphosphate synthase wherein mechanistically non-relevant counter-ion pairing between aza-analogs of early stage carbocation intermediates and pyrophosphate is observed. Thus, (di)terpene synthases seem to mediate specific reaction outcomes, at least in part, by providing stereoelectronic effects to counteract those exerted by the pyrophosphate co-product.

Zhou, Ke; Peters, Reuben J.

2013-01-01

301

Diethyl 4-(2-meth-oxy-phen-yl)-2,6-di-methyl-1,4-di-hydro-pyridine-3,5-di-carboxyl-ate  

PubMed Central

In the title compound, C20H25NO5, the di­hydro­pyridine ring adopts a flattened boat conformation. The meth­oxy­phenyl ring is almost perpendicular to the mean plane of the pyridine ring [dihedral angle = 88.42?(3)°]. The two carbonyl units adopt a synperiplanar conformation with respect to the double bonds in the di­hydro­pyridine ring. In the crystal, mol­ecules are connected by N—H?O hydrogen bonds into R 4 4(24) tetra­meric rings. A region of disordered electron density, located at the center of four adjacent mol­ecules, was treated with the SQUEEZE routine in PLATON [Spek (2009 ?). Acta Cryst. D65, 148–155]. It is probably the result of traces of the solvent of crystallization and was not taken into account during the structure refinement.

Wang, Ke; Wang, Yifeng; Yao, Minjie; Xu, Danqian

2013-01-01

302

MOZ Regulates the Tbx1 Locus, and Moz Mutation Partially Phenocopies DiGeorge Syndrome  

PubMed Central

Summary DiGeorge syndrome, caused by a 22q11 microdeletion or mutation of the TBX1 gene, varies in severity greatly, even among monozygotic twins. Epigenetic phenomena have been invoked to explain phenotypic differences in individuals of identical genetic composition, although specific chromatin modifications relevant to DiGeorge syndrome are elusive. Here we show that lack of the histone acetyltransferase MOZ (MYST3/KAT6A) phenocopies DiGeorge syndrome, and the MOZ complex occupies the Tbx1 locus, promoting its expression and histone 3 lysine 9 acetylation. Importantly, DiGeorge syndrome-like anomalies are present in mice with homozygous mutation of Moz and in heterozygous Moz mutants when combined with Tbx1 haploinsufficiency or oversupply of retinoic acid. Conversely, a Tbx1 transgene rescues the heart phenotype in Moz mutants. Our data reveal a molecular mechanism for a specific chromatin modification of the Tbx1 locus intersecting with an environmental determinant, modeling variability in DiGeorge syndrome.

Voss, Anne K.; Vanyai, Hannah K.; Collin, Caitlin; Dixon, Mathew P.; McLennan, Tamara J.; Sheikh, Bilal N.; Scambler, Peter; Thomas, Tim

2012-01-01

303

Structural insight into the mechanism of cyclic di-GMP hydrolysis by EAL domain phosphodiesterases  

PubMed Central

Cyclic diguanylate (c-di-GMP) is a ubiquitous second messenger regulating diverse cellular functions including motility, biofilm formation, cell cycle progression and virulence in bacteria. In the cell, degradation of c-di-GMP is catalyzed by highly specific EAL domain phosphodiesterases whose catalytic mechanism is still unclear. Here, we purified 13 EAL domain proteins from various organisms and demonstrated that their catalytic activity is associated with the presence of 10 conserved EAL domain residues. The crystal structure of the TDB1265 EAL domain was determined in a free state (1.8 Å) and in complex with c-di-GMP (2.35 Å) and unveiled the role of the conserved residues in substrate binding and catalysis. The structure revealed the presence of two metal ions directly coordinated by six conserved residues, two oxygens of the c-di-GMP phosphate, and potential catalytic water molecule. Our results support a two-metal-ion catalytic mechanism of c-di-GMP hydrolysis by EAL domain phosphodiesterases.

Tchigvintsev, Anatoli; Xu, Xiaohui; Singer, Alexander; Chang, Changsoo; Brown, Greg; Proudfoot, Michael; Cui, Hong; Flick, Robert; Anderson, Wayne F.; Joachimiak, Andrzej; Galperin, Michael Y.; Savchenko, Alexei; Yakunin, Alexander F.

2010-01-01

304

Cyclic di-GMP sensing via the innate immune signaling protein STING.  

PubMed

Detection of foreign materials is the first step of successful immune responses. Stimulator of interferon genes (STING) was shown to directly bind cyclic diguanylate monophosphate (c-di-GMP), a bacterial second messenger, and to elicit strong interferon responses. Here we elucidate the structural features of the cytosolic c-di-GMP binding domain (CBD) of STING and its complex with c-di-GMP. The CBD exhibits an ? + ? fold and is a dimer in the crystal and in solution. Surprisingly, one c-di-GMP molecule binds to the central crevice of a STING dimer, using a series of stacking and hydrogen bonding interactions. We show that STING is autoinhibited by an intramolecular interaction between the CBD and the C-terminal tail (CTT) and that c-di-GMP releases STING from this autoinhibition by displacing the CTT. The structures provide a remarkable example of pathogen-host interactions in which a unique microbial molecule directly engages the innate immune system. PMID:22705373

Yin, Qian; Tian, Yuan; Kabaleeswaran, Venkataraman; Jiang, Xiaomo; Tu, Daqi; Eck, Michael J; Chen, Zhijian J; Wu, Hao

2012-06-14

305

Engineering a novel c-di-GMP-binding protein for biofilm dispersal.  

PubMed

Bacteria prefer to grow attached to themselves or an interface, and it is important for an array of applications to make biofilms disperse. Here we report simultaneously the discovery and protein engineering of BdcA (formerly YjgI) for biofilm dispersal using the universal signal 3,5-cyclic diguanylic acid (c-di-GMP). The bdcA deletion reduced biofilm dispersal, and production of BdcA increased biofilm dispersal to wild-type level. Since BdcA increases motility and extracellular DNA production while decreasing exopolysaccharide, cell length and aggregation, we reasoned that BdcA decreases the concentration of c-di-GMP, the intracellular messenger that controls cell motility through flagellar rotation and biofilm formation through synthesis of curli and cellulose. Consistently, c-di-GMP levels increase upon deleting bdcA, and purified BdcA binds c-di-GMP but does not act as a phosphodiesterase. Additionally, BdcR (formerly YjgJ) is a negative regulator of bdcA. To increase biofilm dispersal, we used protein engineering to evolve BdcA for greater c-di-GMP binding and found that the single amino acid change E50Q causes nearly complete removal of biofilms via dispersal without affecting initial biofilm formation. PMID:21059164

Ma, Qun; Yang, Zhonghua; Pu, Mingming; Peti, Wolfgang; Wood, Thomas K

2010-11-08

306

Engineering a Novel c-di-GMP-Binding Protein for Biofilm Dispersal  

PubMed Central

SUMMARY Bacteria prefer to grow attached to themselves or an interface, and it is important for an array of applications to make biofilms disperse. Here we report simultaneously the discovery and protein engineering of BdcA (formerly YjgI) for biofilm dispersal using the universal signal 3,5-cyclic diguanylic acid (c-di-GMP). The bdcA deletion reduced biofilm dispersal, and production of BdcA increased biofilm dispersal to wild-type levels. Since BdcA increases motility and extracellular DNA production while decreasing exopolysaccharide, cell length, and aggregation, we reasoned that BdcA decreases the concentration of c-di-GMP, the intracellular messenger that controls cell motility through flagellar rotation and biofilm formation through synthesis of curli and cellulose. Consistently, c-di-GMP levels increase upon deleting bdcA, and purified BdcA binds c-di-GMP but does not act as a phosphodiesterase. Additionally, BdcR (formerly YjgJ) is a negative regulator of bdcA. To increase biofilm dispersal, we used protein engineering to evolve BdcA for greater c-di-GMP binding and found that the single amino acid change E50Q causes nearly complete removal of biofilms via dispersal without affecting initial biofilm formation.

Ma, Qun; Yang, Zhonghua; Pu, Mingming; Peti, Wolfgang; Wood, Thomas K.

2010-01-01

307

Federico di Montefeltro's hyperkyphosis: a visual-historical case report  

PubMed Central

Introduction The literature contains several publications describing the use of visual arts to develop observational skills in medical students. Portraits of individuals of the Italian Renaissance can be used to enhance these skills and stimulate the development of differential diagnoses in medical students. The Duke of Urbino, Federico di Montefeltro (1422–1482), lost his right eye and nasal bridge during a jousting accident in 1450. Consequently, almost every profile of him in existence today depicts his face in a left lateral view. Although some authors have described the Duke's missing nasal bridge, none have described his prominent thoracic hyperkyphosis, which is clearly discernible in two paintings by Piero della Francesca. The purpose of this report is to describe the Duke's hyperkyphosis, develop relevant differential diagnoses, and suggest a possible etiology of the convexity. Case presentation We have examined two paintings of the Duke by Piero della Francesca – the diptych, The Duke and Duchess of Urbino (1465), and the Madonna of the Egg (1472). A MEDLINE search revealed 2 articles that were relevant to this study. This search was complemented by a search of the collection at the library of Seton Hall University, and the first author's experience studying at the University of Urbino. The historical data obtained from these searches were incorporated with the visual analysis to formulate a plausible etiology of the Duke's thoracic hyperkyphosis. Conclusion Differential diagnoses of the Duke's thoracic hyperkyphosis include Scheuermann disease, osteoporosis, and trauma-related spinal changes. Based on the available evidence, the Duke's thoracic hyperkyphosis could have been caused by repetitive trauma to the spine due to numerous hours on horseback with heavy armor. The role that osteoporosis played in the development of the hyperkyphosis is unclear, as is whether the Duke had the convexity during childhood. The hyperkyphosis as a stylistic variant by Piero della Francesca is unlikely. This report is an example of a teaching strategy that can be used to enhance the observational skills of medical students in evidence-based medical education.

D'Antoni, Anthony V; Terzulli, Stephanie L

2008-01-01

308

Di-(2-ethylhexyl) phthalate and autism spectrum disorders  

PubMed Central

ASDs (autism spectrum disorders) are a complex group of neurodevelopment disorders, still poorly understood, steadily rising in frequency and treatment refractory. Extensive research has been so far unable to explain the aetiology of this condition, whereas a growing body of evidence suggests the involvement of environmental factors. Phthalates, given their extensive use and their persistence, are ubiquitous environmental contaminants. They are EDs (endocrine disruptors) suspected to interfere with neurodevelopment. Therefore they represent interesting candidate risk factors for ASD pathogenesis. The aim of this study was to evaluate the levels of the primary and secondary metabolites of DEHP [di-(2-ethylhexyl) phthalate] in children with ASD. A total of 48 children with ASD (male: 36, female: 12; mean age: 11±5 years) and age- and sex-comparable 45 HCs (healthy controls; male: 25, female: 20; mean age: 12±5 years) were enrolled. A diagnostic methodology, based on the determination of urinary concentrations of DEHP metabolites by HPLC-ESI-MS (HPLC electrospray ionization MS), was applied to urine spot samples. MEHP [mono-(2-ethylhexenyl) 1,2-benzenedicarboxylate], 6-OH-MEHP [mono-(2-ethyl-6-hydroxyhexyl) 1,2-benzenedicarboxylate], 5-OH-MEHP [mono-(2-ethyl-5-hydroxyhexyl) 1,2-benzenedicarboxylate] and 5-oxo-MEHP [mono-(2-ethyl-5-oxohexyl) 1,2-benzenedicarboxylate] were measured and compared with unequivocally characterized, pure synthetic compounds (>98%) taken as standard. In ASD patients, significant increase in 5-OH-MEHP (52.1%, median 0.18) and 5-oxo-MEHP (46.0%, median 0.096) urinary concentrations were detected, with a significant positive correlation between 5-OH-MEHP and 5-oxo-MEHP (rs?=?0.668, P<0.0001). The fully oxidized form 5-oxo-MEHP showed 91.1% specificity in identifying patients with ASDs. Our findings demonstrate for the first time an association between phthalates exposure and ASDs, thus suggesting a previously unrecognized role for these ubiquitous environmental contaminants in the pathogenesis of autism.

Testa, Chiara; Nuti, Francesca; Hayek, Joussef; De Felice, Claudio; Chelli, Mario; Rovero, Paolo; Latini, Giuseppe; Papini, Anna Maria

2012-01-01

309

6,8-Di-bromo-3-nitro-2-phenyl-2H-chromene  

PubMed Central

In the title compound, C15H9Br2NO3, the chromene unit is not quite planar (r.m.s. deviation from planarity = 0.0888?Å). The di­hydro­pyran ring adopts an envelope conformation with the phenyl-substituted C atom fused to the di­hydro­pyran ring as the flap. The dihedral angle between the plane defined by this C atom and the adjacent C and O atoms and the mean plane of the di­hydro­pyran ring excluding the phenyl-substituted C atom is 25.1?(3)°. The dihedral angle between the mean plane of the chromene unit and the phenyl ring is 85.7?(1)°. The crystal structure features C—H?O hydrogen bonds and Br?O contacts [3.289?(3)?Å] involving the nitro O atoms.

Yan, Lin-Jie; Zhang, Sheng-Yong

2013-01-01

310

Electron spin resonance study of perturbed di-interstitials in diamond  

NASA Astrophysics Data System (ADS)

Twelve satellite centres, labelled as R1A-L, were detected and characterised by electron spin resonance around the R1 centre, which was previously identified with the di-interstitial in diamond. The R1 satellites exhibit spin S = 1 and monoclinic-I symmetry; their spin-spin interaction constant D is 4% smaller, but the anisotropy parameter E is significantly larger than that of the R1 centre. All the satellites anneal out at a temperature in the range 300-350 °C, which is slightly lower than that of the R1 centre. However, the R1 centre, but not its satellites, can be restored by UV illumination after annealing at temperatures as high as 800 °C. The experimental observations can be accounted for by a model assigning the R1A..L centres to distinct pairs of a di-interstitial and a neutral vacancy located in the {110} symmetry plane of the di-interstitial.

Iakoubovskii, K.; Baker, J. M.; Newton, M. E.

2004-09-01

311

MauG: a di-heme enzyme required for methylamine dehydrogenase maturation.  

PubMed

Methylamine dehydrogenase (MADH) requires the cofactor tryptophan tryptophylquinone (TTQ) for activity. TTQ is a posttranslational modification that results from an 8-electron oxidation of two specific tryptophans in the MADH ?-subunit. The final 6-electron oxidation is catalyzed by an unusual c-type di-heme enzyme, MauG. The di-ferric enzyme can react with H(2)O(2), but atypically for c-type hemes the di-ferrous enzyme can react with O(2) as well. In both cases, an unprecedented bis-Fe(IV) redox state is formed, composed of a ferryl heme (Fe(IV)=O) with the second heme as Fe(IV) stabilized by His-Tyr axial ligation. Bis-Fe(IV) MauG acts as a potent 2-electron oxidant. Catalysis is long-range and requires a hole hopping electron transfer mechanism. This review highlights the current knowledge and focus of research into this fascinating system. PMID:23086017

Wilmot, Carrie M; Yukl, Erik T

2012-10-22

312

DiPOLE: A multi-slab cryogenic diode pumped Yb:YAG amplifier  

NASA Astrophysics Data System (ADS)

The Diode Pumped Optical Laser Experiments (DiPOLE) project at Central Laser Facility (CLF) is aimed at the development of scalable diode pumped, cryogenic gas cooled, multi-slab Yb:YAG amplifiers. Optimized designs for amplifiers capable of generating kJ pulse energies at multi-Hz repetition rate have been finalised and development of a scaled-down 10J, 10Hz prototype is currently underway at the CLF. We report on the recent results obtained on a 4-pass bowtie as well as 6-pass image relaying multi-pass setup for the DiPOLE amplifier. Additionally, Preliminary results for the amplifier performance with uniform doping (3 × 2at%) compared to gradient doping (2 × 1at% + 2 × 2at%) at cryogenic temperatures, confirms the multi-slab gradient doped design adopted for DiPOLE amplifier.

Banerjee, Saumyabrata; Ertel, Klaus; Mason, Paul; Phillips, Jonathan; Greenhalgh, Justin; Collier, John

2013-05-01

313

Analysis of Class E low di/dt rectifier with a series inductor  

NASA Astrophysics Data System (ADS)

An analysis along with an experimental verification is presented for a Class E zero-current-switching (low di/dt) rectifier. The circuit consists of a rectifier diode, an inductor connected in series with a diode, and a filter capacitor. The addition of the series inductor allows the rectifier to decouple the diode ON duty cycle and the output ripple voltage. The series inductor sets the value of the diode ON duty cycle which can be large, e.g., 0.5. The filter capacitor independently sets the level of the ripple voltage. The rectifier diode turns on at zero di/dt and low dv/dt and turns off at low /di/dt/, reducing switching losses, switching noise, and reverse-recovery current. The experimental results confirmed theoretical predictions. Other topologies of unregulated and regulated synchronous rectifiers are also discussed.

Kazimierczuk, Marian K.; Szaraniec, Wojciech

1993-01-01

314

Agar hydrogel with silver nanoparticles to prolong the shelf life of Fior di Latte cheese.  

PubMed

The objective of this work was to evaluate the effectiveness of an antimicrobial packaging system containing active nanoparticles on the quality deterioration of Fior di Latte cheese. To this aim, 3 concentrations of silver montmorillonite embedded in agar were used. The cell loads of spoilage and useful microorganisms were monitored during a refrigerated storage period. Moreover, cheese sensory quality (i.e., odor, color, consistency, and overall quality) was evaluated by means of a panel test. Results showed that the active packaging system markedly increased the shelf life of Fior di Latte cheese, due to the ability of silver cations to control microbial proliferation, without affecting the functional dairy microbiota and the sensory characteristics of the product. The active packaging system developed in this work could be used to prolong the shelf life of Fior di Latte and boost its distribution beyond local market borders. PMID:21426957

Incoronato, A L; Conte, A; Buonocore, G G; Del Nobile, M A

2011-04-01

315

c-di-AMP Secreted by Intracellular Listeria monocytogenes Activates a Host Type I Interferon Response  

PubMed Central

Intracellular bacterial pathogens, such as Listeria monocytogenes, are detected in the cytosol of host immune cells. Induction of this host response is often dependent on microbial secretion systems, and in L. monocytogenes, is dependent on multi-drug efflux pumps (MDRs). Using L. monocytogenes mutants that over-expressed MDRs, we identified cyclic diadenosine monophosphate (c-di-AMP) as a secreted molecule able to trigger the cytosolic host response. Over-expression of the di-adenylate cyclase, dacA (lmo2120), resulted in elevated levels of the host response during infection. C-di-AMP thus represents a putative bacterial secondary signaling molecule that triggers a cytosolic pathway of innate immunity and is predicted to be present in a wide variety of bacteria and archea.

Woodward, Joshua J.; Iavarone, Anthony T.; Portnoy, Daniel A.

2011-01-01

316

Aqueous phase oxidation of methane with hydrogen peroxide catalyzed by di-iron-substituted silicotungstate  

SciTech Connect

The active site of methane monooxygenase has been shown to have a {mu}-hydroxo di-iron structure. Among alkanes, the activation and functionalization of methane have attracted much attention because of its abundance and low reactivity. Recently, there has been a renewed interest in the direct conversion of methane into oxygenates with environmentally clean oxidants such as molecular oxygen and hydrogen peroxide. The use of water as a solvent in homogeneous transition-metal catalysis has been of growing importance because of its environmentally friendly nature. In these aspects, catalytic oxygenation of methane with hydrogen peroxide in water by model complex catalysts with di-iron centers is of great interest but has been unsuccessful due to the catalyst instability (particularly organic ligands) towards hydrogen peroxide and insolubility in water. In this paper, the authors attempt to demonstrate catalytic oxidation of methane with hydrogen peroxide in water by di-iron-substituted silicotungstate.

Mizuno, Noritaka; Seki, Yasuhiro; Nishiyama, Yoshiyuki; Kiyoto, Ikuro; Misono, Makoto [Univ of Tokyo (Japan). Dept. of Applied Chemistry

1999-06-10

317

Interface formation of Mg with DiMePTCDI studied by Raman spectroscopy  

NASA Astrophysics Data System (ADS)

Metal/organic interfaces play an important role in the performance of organic based devices. In this work the interface formation between Mg and N,N-DiMethyl-3,4,9,10-Perylene Tetra Carboxylic DiImide (DiMePTCDI) films grown on sulphur-passivated GaAs (100) substrates is investigated in situ by Raman spectroscopy. The Raman spectra are taken in a backscattering geometry at room temperature under resonance conditions with the 488 nm (2.54 eV) Ar+ laser line. They reveal that when Mg is deposited onto a 15 nm DiMePTCDI layer the external phonon modes are preserved up to large metal coverages. Since these modes are a fingerprint of the molecular crystal, their preservation indicates a low diffusion of Mg into the DiMePTCDI layer. Concerning the internal molecular modes, the Mg deposition induces a breakdown of selection rules which is proposed to originate from a dynamical charge transfer between the DiMePTCDI molecules and the metal. Above 0.3 nm nominal thickness of Mg the line-shape of the molecular breathing mode at 221 cm-1 develops an asymmetric tail towards higher frequencies. A similar effect is observed for the bands that occur at 1291 cm-1 and 1606 cm-1 but the asymmetry appears at the low frequency side. This line-shape asymmetry is likely to be related to a Fano resonant coupling between the molecular vibrational modes and the electronic continuum of states of metallic clusters formed above 0.3 nm Mg coverage.

Paez, B. A.; Salvan, G.; Scholz, R.; Zahn, D. R. T.

2005-12-01

318

DI2ADEM: an adaptive hypermedia designed to improve access to relevant medical information.  

PubMed Central

The World Wide Web (web) provides the same type of information to widely different users and these users must then find the information suitable for their use in the package offered. The authors present the DI2ADEM project designed to take the user into account and intended to provide this user with appropriate medical information. To do that, DI2ADEM is suggesting an adaptive hypermedia based on the management of a meta-knowledge of the user and a knowledge of the information that can be circulated. An adaptive hypermedia prototype devoted to paediatric oncology was implemented on the intranet network of a university hospital.

Pagesy, R.; Soula, G.; Fieschi, M.

2000-01-01

319

ACE D/I polymorphism, migraine, and cardiovascular disease in women  

PubMed Central

Background: Interrelationships among the ACE deletion/insertion (D/I) polymorphism (rs1799752), migraine, and cardiovascular disease (CVD) are biologically plausible but remain controversial. Methods: Association study among 25,000 white US women, participating in the Women’s Health Study, with information on the ACE D/I polymorphism. Migraine and migraine aura status were self-reported. Incident CVD events were confirmed after medical record review. We used logistic regression to investigate the genotype-migraine association and proportional hazards models to evaluate the interrelationship among genotype, migraine, and incident CVD. Results: At baseline, 4,577 (18.3%) women reported history of migraine; 39.5% of the 3,226 women with active migraine indicated aura. During 11.9 years of follow-up, 625 CVD events occurred. We did not find an association of the ACE D/I polymorphism with migraine or migraine aura status. There was a lack of association between the ACE D/I polymorphism and incident major CVD, ischemic stroke, and myocardial infarction. Migraine with aura doubled the risk for CVD, but only for carriers of the DD (multivariable-adjusted relative risk [RR] = 2.10; 95% CI = 1.22–3.59; p = 0.007) and DI genotype (multivariable-adjusted RR = 2.31; 95% CI = 1.52–3.51; p < 0.0001). The risk was not significant among carriers of the II genotype, a pattern we observed for myocardial infarction and ischemic stroke. Conclusions: Data from this large cohort of women do not suggest an association of the ACE deletion/insertion (D/I) polymorphism with migraine, migraine aura status, or cardiovascular disease (CVD). The increased risk for CVD among migraineurs with aura was only apparent for carriers of the DD/DI genotype. Due to limited number of outcome events, however, future studies are warranted to further investigate this association. GLOSSARY ACE = angiotensin-converting enzyme; CI = confidence interval; CVD = cardiovascular disease; D/I = deletion/insertion; HRs = hazard ratios; IHS = International Headache Society; MI = myocardial infarction; OR = odds ratio; WHS = Women’s Health Study.

Schurks, M; Zee, R Y.L.; Buring, J E.; Kurth, T

2009-01-01

320

A new di-O-prenylated flavone from an actinomycete Streptomyces sp. MA-12.  

PubMed

A new di-O-prenylated flavone, named 7,3'-di-(?,?-dimethylallyloxy)-5-hydroxy-4'-methoxyflavone (1), was isolated from the culture broth of the endophytic actinomycete Streptomyces sp. MA-12 isolated from the root of the semi-mangrove plant Myoporum bontioides A. Gray. The structure of 1 was determined by comprehensive spectroscopic methods, including 1D and 2D NMR experiments (COSY, HMQC, and HMBC). Primary bioassays showed that 1 at concentration of 0.25 mM had moderate inhibitory activity against three plant pathogenic fungi including Colletotrichum musae, Gibberella zeae (Schweinitz) Petch, and Penicillium citrinum Thom. PMID:23323861

Ding, Wei-Jia; Zhang, Sui-Qiang; Wang, Jin-Hua; Lin, Yue-Xia; Liang, Qiao-Xia; Zhao, Wei-Jian; Li, Chun-Yuan

2013-01-17

321

Substituted pyrazine-di-N-oxides as the mediators of catalytic oxidation of organic compounds  

Microsoft Academic Search

The mechanism of oxidation of 2,5-dimethyl-, 2,3,5,6-tetramethyl-, 2,3-dimethyl-5,6-cyclohexa, and 3-phenyl-5,6-cyclohexapyrazine-di-N-oxides\\u000a is studied by cyclic voltammetry, quantum chemical simulations, and ESR electrolysis. The studies are carried out on electrodes\\u000a of glassy carbon and Pt in 0.1 M LiClO4 solutions in acetonitrile. ESR spectra of radical cations of substituted pyrazine-di-N-oxides are recorded. The effects of\\u000a the temperature, oxygen, and the additions of

S. I. Kulakovskaya; A. V. Kulikov; A. F. Shestakov

2007-01-01

322

Anti-site pair in SiC: a model of the DI center  

NASA Astrophysics Data System (ADS)

The DI low-temperature photoluminescence center is a well-known defect stable up to 1700°C annealing in SiC, still its structure is not known after decades of study. Combining experimental and theoretical studies in this paper we will show that the properties of an anti-site pair can reproduce the measured one-electron level position and local vibration modes of the DI center and the model is consistent with other experimental findings as well. We give theoretical values of the hyperfine constants of the anti-site pair in its paramagnetic state as a means to confirm our model.

Gali, A.; Deák, P.; Rauls, E.; Son, N. T.; Ivanov, I. G.; Carlsson, F. H. C.; Janzén, E.; Choyke, W. J.

2003-12-01

323

3D numerical simulation of fuel injection and combustion phenomena in Di diesel engines  

SciTech Connect

In this paper, the calculations of combustion phenomena in DI diesel engines are performed by modifying the KIVA program so as to be applicable to multi-hole nozzles and arbitrary patterns of injection rate. The thermophysical and thermochemical properties of gasoline are altered to those diesel fuel. In order to investigate the ability of this modified program, the calculations are compared with the experiments on single cylinder engines. The calculation for the heavy duty DI diesel engine is performed with this diesel combustion program. From this calculation results the gas flow, spray shapes, air-fuel mixing process, and combustion in this engine are investigated.

Takenaka, Y.; Aoyagi, Y.; Tsuji, Y.; Joko, I.

1989-01-01

324

MedSynDiKATe--design considerations for an ontology-based medical text understanding system.  

PubMed Central

MedSynDiKATe is a natural language processor for automatically acquiring knowledge from medical finding reports. The content of these documents is transferred to formal representation structures which constitute a corresponding text knowledge base. The general system architecture we present integrates requirements from the analysis of single sentences, as well as those of referentially linked sentences forming cohesive texts. The strong demands MedSynDiKATe poses to the availability of expressive knowledge sources are accounted for by two alternative approaches to (semi)automatic ontology engineering.

Hahn, U.; Romacker, M.; Schulz, S.

2000-01-01

325

catena-Poly[[[tetra-aqua-magnesium]-trans-?-[(piperazine-1,4-diium-1,4-di-yl)bis-(methyl-ene)]di-phospho-nato] hemihydrate  

PubMed Central

The structure of the title polymer, }[Mg(C6H14N2O6P2)(H2O)4]·0.5H2O}n, is based on centrosymmetric MgO6 octahedra, which are linked by [(piperazine-1,4-diium-1,4-di­yl)bis­(methyl­ene)]di­phospho­nate ligands, forming chains parallel to [1-1-1]. These chains are connected via hydrogen bonds primarily formed between the phospho­nate groups and water mol­ecules. The latter constitute four of the corners of the MgO6 polyhedra and bind to the O atoms of the phospho­nate groups of neighbouring chains. The lattice water molecule is disordered around an inversion centre, exhibiting an occupancy of 0.25.

Schilling, Lars-Hendrik; Stock, Norbert

2013-01-01

326

(2-tert-Butyl-3-phenyl-2,3-di-hydro-isoxazole-4,5-di-yl)bis-(phenyl-methanone)  

PubMed Central

The phenyl and tert-butyl groups of the title compound, C27H25NO3, exhibit a trans configuration in agreement with the stereochemistry of the Z phenyl-N-tert-butyl­nitrone starting material. The attached carbonyl groups are not coplanar with the neighboring di­hydro­isoxazole ring and the phenyl rings they are bonded to, with torsion angles of 59.26?(8), 17.53?(11), 16.52?(12) and 52.86?(7)°. The dihedral angle between the di­hydro­isoxazole ring and the directly attached phenyl group is 86.86?(8)°. There are two nonclassical inter­molecular C—H?O hydrogen-bonding inter­actions that operate together with an inter­molecular C—H?? inter­action to form a supramolecular architecture in the crystal system.

Sandhya, R.; Sithambaresan, M.; Prathapan, S.; Kurup, M. R. Prathapachandra

2013-01-01

327

Gold Recovery from Acidic Leach Solutions Using as Extractants Trialkylamines of N,N'-Di-Alkyl-Aliphatic Amides.  

National Technical Information Service (NTIS)

TriOctylAmine (TOA) and a di-substituted aliphatic amide, N,N-Di-N-ButylOctanamide (DBOA), were examined in batch and in mini mixer-settler experiments using leachates of Peruvian and Bolivian concentrates. With these minerals, very rich in sulfur (pyrite...

F. Baroncelli D. Carlini G. M. Gasparini E. Simonetti

1988-01-01

328

Structural and biochemical characterization of linear dinucleotide analogues bound to the c-di-GMP-I aptamer.  

PubMed

The cyclic dinucleotide c-di-GMP regulates lifestyle transitions in many bacteria, such as the change from a free motile state to a biofilm-forming community. Riboswitches that bind this second messenger are important downstream targets in this bacterial signaling pathway. The breakdown of c-di-GMP in the cell is accomplished enzymatically and results in the linear dinucleotide pGpG. The c-di-GMP-binding riboswitches must be able to discriminate between their cognate cyclic ligand and linear dinucleotides in order to be selective biological switches. It has been reported that the c-di-GMP-I riboswitch binds c-di-GMP 5 orders of magnitude better than the linear pGpG, but the cause of this large energetic difference in binding is unknown. Here we report binding data and crystal structures of several linear c-di-GMP analogues in complex with the c-di-GMP-I riboswitch. These data reveal the parameters for phosphate recognition and the structural basis of linear dinucleotide binding to the riboswitch. Additionally, the pH dependence of binding shows that exclusion of pGpG is not due to the additional negative charge on the ligand. These data reveal principles that, along with published work, will contribute to the design of c-di-GMP analogues with properties desirable for use as chemical tools and potential therapeutics. PMID:22148472

Smith, Kathryn D; Lipchock, Sarah V; Strobel, Scott A

2011-12-27

329

Health hazards associated with the use of di-(2-ethylhexyl) phthalate (commonly referred to as DOP) in HEPA filter test.  

National Technical Information Service (NTIS)

Di-(2-ethylhexyl) phthalate (DEHP), commonly referred to as di-octyl phthalate, is an important production chemical in the US. In addition to its major use as an additive in plastics, DEHP is widely used to evaluate the effectiveness of high efficiency pa...

1995-01-01

330

Analysis of a Borrelia burgdorferi phosphodiesterase demonstrates a role for cyclic-di-GMP in motility and virulence  

PubMed Central

Summary The genome of Borrelia burgdorferi encodes a set of genes putatively involved in cyclic-di-GMP metabolism. Although BB0419 was shown to be a diguanylate cyclase, the extent to which bb0419 or any of the putative cyclic-di-GMP metabolizing genes impact B. burgdorferi motility and pathogenesis has not yet been reported. Here we identify and characterize a phosphodiesterase (BB0363). BB0363 specifically hydrolyzed cyclic-di-GMP with a Km of 0.054 ?M, confirming it is a functional cyclic-di-GMP phosphodiesterase. A targeted mutation in bb0363 was constructed using a newly developed promoterless antibiotic cassette that does not affect downstream gene expression. The mutant cells exhibited an altered swimming pattern, indicating a function for cyclic-di-GMP in regulating B. burgdorferi motility. Furthermore, the bb0363 mutant cells were not infectious in mice, demonstrating an important role for cyclic-di-GMP in B. burgdorferi infection. The mutant cells were able to survive within Ixodes scapularis ticks after a blood meal from naïve mice; however, ticks infected with the mutant cells were not able to infect naïve mice. Both motility and infection phenotypes were restored upon genetic complementation. These results reveal an important connection between cyclic-di-GMP, B. burgdorferi motility and Lyme disease pathogenesis. A mechanism by which cyclic-di-GMP influences motility and infection is proposed.

Sultan, Syed Z.; Pitzer, Joshua E.; Miller, Michael R.; Motaleb, Md. A.

2010-01-01

331

40 CFR 721.1550 - Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic acid (1:1).  

Code of Federal Regulations, 2013 CFR

...Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic...Benzenediazonium, 4-(di-methyl-amino)-, salt with 2-hy-droxy-5-sul-fo-benzoic...benzenediazonium, 4-(dimethylamino)-, salt with...

2013-07-01

332

40 CFR 721.2275 - N,N,Nâ²,Nâ²-Tetrakis(oxi-ranyl- methyl)-1,3-cyclohexane di-meth-anamine.  

Code of Federal Regulations, 2010 CFR

...Tetrakis(oxi-ranyl- methyl)-1,3-cyclohexane di-meth-anamine. 721.2275 Section 721.2275 Protection of...Tetrakis(oxi-ranyl- methyl)-1,3-cyclohexane di-meth-anamine. (a) Chemical substances and significant...

2010-07-01

333

40 CFR 721.2275 - N,N,Nâ²,Nâ²-Tetrakis(oxi-ranyl- methyl)-1,3-cyclohexane di-meth-anamine.  

Code of Federal Regulations, 2010 CFR

...Tetrakis(oxi-ranyl- methyl)-1,3-cyclohexane di-meth-anamine. 721.2275 Section 721.2275 Protection of...Tetrakis(oxi-ranyl- methyl)-1,3-cyclohexane di-meth-anamine. (a) Chemical substances and significant...

2009-07-01

334

L'impatto delle Tecnologie Informative e della Comunicazione (ICT) sui Fabbisogni Formativi delle Imprese Fornitrici di Servizi Logistici in Italia e in Irlanda  

Microsoft Academic Search

Le innovazioni connesse all’utilizzo tecnologie informative e della comunicazione (ICT) hanno caratterizzato il cambiamento nell’industria dei servizi logistici e di trasporto nel corso degli ultimi anni. In questo processo, la formazione e lo sviluppo di nuove competenze professionali assumono un ruolo sempre più centrale. Loscopo di questo lavoro è di studiare l’impatto delle ICT sui fabbisogni formativi delle imprese logistiche

P Evangelista; Edward Sweeney; Francesco Andreottola

2003-01-01

335

Comorbidità psichiatrica nelle dipendenze. Studio su un campione di pazienti afferenti al Dipartimento per le Dipendenze* Psychiatric co-morbidity in drug dependence  

Microsoft Academic Search

Riassunto Il presente articolo costituisce una ricerca sulla comorbidità psichiatrica svolta presso il Dipartimento per Dipendenze di Pa- dova, volta ad indagare le condizioni di vita, la condizione psicologica e infine il tasso di prevalenza dei disturbi mentali nella popolazione tossicomanica. Lo studio ha utilizzato due strumenti di indagine: il questionario EuropASI e l'intervista semistrutturata CIDI-C a cui sono stati

LUCIA DI FURIA; M ILENA PIZZA; F ABIANO CAVARZERAN; M ATTEO RIZZO; N ATALIA SAVANI; F INIZIA SCIVITTARO

336

40 CFR 721.9516 - Siloxanes and silicones, 3-[(2-aminoethyl) amino]propyl Me, di-Me, reaction products with...  

Code of Federal Regulations, 2010 CFR

...2-aminoethyl) amino]propyl Me, di-Me, reaction products with polyethylene-polypropylene...2-aminoethyl) amino]propyl Me, di-Me, reaction products with polyethylene-polypropylene...2-aminoethyl) amino]propyl Me, di-Me, reaction products with...

2009-07-01

337

Laccase-mediator system in the decolorization of diVerent types of recalcitrant dyes  

Microsoft Academic Search

Phloroglucinol, thymol, and violuric acid (VIO) were selected as laccase mediators after screening 14 diVer- ent compounds with indigo carmine (indigoid dye) as a substrate. With the presence of these three mediators, a nearly complete decolorization (90-100%) was attained in 1 h. Thus, these three compounds were used as mediators for the decolorization of other four dyes. The results indi-

Mei Rong Hu; Ya Peng Chao; Guo Qing Zhang; Zhi Quan Xue; Shijun Qian

338

THE EXTRACTION OF THE LANTHANIDE ELEMENTS FROM PERCHLORIC ACID BY DI(2- ETHYLHEXYL) HYDROGEN PHOSPHATE  

Microsoft Academic Search

The elements lanthanum, cerium, praseodymium, neodymium, promethium, ; samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ; ytterbium, lutetium, and yttrium were extracted from an aqueous perchlorate phase ; at different acidities into a solution of di-(2-ethylhexyl) hydrogen phosphate in ; toluene. Separation factors for all the elements of the lanthanide series were ; calculated, and the average value for

T. B. Pierce; P. F. Peck

1963-01-01

339

MONO-(3-CARBOXYPROPYL) PHTHALATE, A METABOLITE OF DI-N-OCTYL PHTHALATE  

EPA Science Inventory

Di-n-octyl phthalate (DnOP) is found as a component of mixed C6â??C10 linear-chain phthalates used as plasticizers in various polyvinyl chloride applications, including flooring and carpet tiles. Following exposure and absorption, DnOP is metabolized to its hydrolytic monoester, mo...

340

Functional characterization of dI6 interneurons in the neonatal mouse spinal cord  

PubMed Central

Our understanding of the neural control of locomotion has been greatly enhanced by the ability to identify and manipulate genetically defined populations of interneurons that comprise the locomotor central pattern generator (CPG). To date, the dI6 interneurons are one of the few populations that settle in the ventral region of the postnatal spinal cord that have not been investigated. In the present study, we utilized a novel transgenic mouse line to electrophysiologically characterize dI6 interneurons located close to the central canal and study their function during fictive locomotion. The majority of dI6 cells investigated were found to be rhythmically active during fictive locomotion and could be divided into two electrophysiologically distinct populations of interneurons. The first population fired rhythmic trains of action potentials that were loosely coupled to ventral root output and contained several intrinsic membrane properties of rhythm-generating neurons, raising the possibility that these cells may be involved in the generation of rhythmic activity in the locomotor CPG. The second population fired rhythmic trains of action potentials that were tightly coupled to ventral root output and lacked intrinsic oscillatory mechanisms, indicating that these neurons may be driven by a rhythm-generating network. Together these results indicate that dI6 neurons comprise an important component of the locomotor CPG that participate in multiple facets of motor behavior.

Dyck, Jason; Lanuza, Guillermo M.

2012-01-01

341

The Theory behind the Theory in DCT and SCDT: A Response to Rigazio-DiGilio.  

ERIC Educational Resources Information Center

Responds to previous article by Rigazio-DiGilio on Developmental Counseling and Therapy and Systemic Cognitive-Developmental Therapy as two integrative models that unify individual, family, and network treatment within coconstructive-developmental framework. Discusses hidden complexities in cognitive-developmental ecosystemic integration and…

Terry, Linda L.

1994-01-01

342

Genetic Modifiers of the Physical Malformations in Velo-Cardio-Facial Syndrome/DiGeorge Syndrome  

ERIC Educational Resources Information Center

|Velo-cardio-facial syndrome/DiGeorge syndrome (VCFS/DGS), the most common micro-deletion disorder in humans, is characterized by craniofacial, parathyroid, and thymic defects as well as cardiac outflow tract malformations. Most patients have a similar hemizygous 3 million base pair deletion on 22q11.2. Studies in mouse have shown that "Tbx1", a…

Aggarwal, Vimla S.; Morrow, Bernice E.

2008-01-01

343

Three Week Intravenous Administration in the Dog DI (2 - Ethylhexyl) Phthalate.  

National Technical Information Service (NTIS)

A repeated dose of approximately 0.50, 0.75, and 1.4 mg/kg/day of di(2-ethylhexyl) phthalate in canine plasma was administered to beagles for 21 days. No consistent compound-related alterations were observed in animal behavior or hematological and clinica...

H. A. Rutter

1975-01-01

344

A potential early middle Pleistocene tephrostratotype for the Mediterranean basin: the Vallo Di Diano, Campania, Italy  

Microsoft Academic Search

A 207-m-long core was taken from the deposits of a paleolake formed in the Vallo di Diano tectonic basin of the southern Apennines, Italy. Fourteen major tephra beds were identified and distributed into three groups based on stratigraphic position, and chemical and mineralogical compositions. In a pilot dating program, glass ages were determined for three of these layers using the

D. B. Karner; E. Juvigne; L. Brancaccio; A. Cinque; E. Russo Ermolli; N. Santangelo; S. Bernasconi; L. Lirer

1999-01-01

345

Beyond “more is better”: Ecological footprint accounting for tourism and consumption in Val di Merse, Italy  

Microsoft Academic Search

Habits of conservation, consumption and recycling are important determinants of economic throughput. Provincial governments interested in tourism's role in a diverse, steady-state economy may wish to orient tourism development around the tourist segments with less intensive consumption habits. We estimate consumption of energy and materials by tourists vacationing in Val di Merse, a rural region of Tuscany, Italy. We compare

Trista M. Patterson; Valentina Niccolucci; Simone Bastianoni

2007-01-01

346

Di(2 ethylhexyl) phthalate and flutamide alter gene expression in the testis of immature male rats  

Microsoft Academic Search

We previously demonstrated that the androgenic and anti-androgenic effects of endocrine disruptors (EDs) alter reproductive function and exert distinct effects on developing male reproductive organs. To further investigate these effects, we used an immature rat model to examine the effects of di-(2 ethylhexyl) phthalate (DEHP) and flutamide (Flu) on the male reproductive system. Immature male SD rats were treated daily

Thuy TB Vo; Eui-Man Jung; Vu Hoang Dang; Yeong-Min Yoo; Kyung-Chul Choi; Frank H Yu; Eui-Bae Jeung

2009-01-01

347

Circuits for grasping: spinal dI3 interneurons mediate cutaneous control of motor behavior.  

PubMed

Accurate motor performance depends on the integration in spinal microcircuits of sensory feedback information. Hand grasp is a skilled motor behavior known to require cutaneous sensory feedback, but spinal microcircuits that process and relay this feedback to the motor system have not been defined. We sought to define classes of spinal interneurons involved in the cutaneous control of hand grasp in mice and to show that dI3 interneurons, a class of dorsal spinal interneurons marked by the expression of Isl1, convey input from low threshold cutaneous afferents to motoneurons. Mice in which the output of dI3 interneurons has been inactivated exhibit deficits in motor tasks that rely on cutaneous afferent input. Most strikingly, the ability to maintain grip strength in response to increasing load is lost following genetic silencing of dI3 interneuron output. Thus, spinal microcircuits that integrate cutaneous feedback crucial for paw grip rely on the intermediary role of dI3 interneurons. PMID:23583114

Bui, Tuan V; Akay, Turgay; Loubani, Osama; Hnasko, Thomas S; Jessell, Thomas M; Brownstone, Robert M

2013-04-10

348

186 Improving Robustness of Spray Guided DI Combustion Systems: The Air-Assisted Approach  

Microsoft Academic Search

Although the direct injection gasoline engine offers the potential for large fuel consumption reductions in passenger vehicles, the wide spread adoption of this technology has been slow. With the onset of ever increasingly stringent emissions legislation requiring the reduction of exhaust pollutants, the DI combustion system for the future must therefore exhibit the favourable combination of significant fuel consumption reduction

Geoffrey CATHCART

349

Selective intraportal transplantation of DiI-marked isolated rat hepatocytes  

Microsoft Academic Search

Transplantation of isolated hepatocytes is a promising alternative to orthotopic liver transplantation in experimental animal models with acute hepatic failure and hereditary enzyme defects. Conventional light microscopy identification of hepatocytes within recipient livers has been limited due to the inability to distinguish between donor and recipient liver cells. In this study, we labeled hepatocytes intracellularly with the fluorescent dye DiI-18

P. M. Markus; S. Koenig; P. Krause; H. Becker

1997-01-01

350

Early Retirement and DI\\/SSI Applications: Exploring the Impact of Depression  

Microsoft Academic Search

Disability among older Americans has declined significantly over the past thirty years. However, claims on the public disability benefit programs have doubled in the past decade. Mental illness is the primary cause of public disability program claims and receipt. This paper investigates the direct and indirect impact of depression on public disability insurance (DI) applications and early retirement behavior among

Rena M. Conti; Ernst R. Berndt; Richard G. Frank

2009-01-01

351

Experimental investigation to develop a methodology for estimating the compression condition of DI Diesel engines  

Microsoft Academic Search

An experimental investigation is conducted to examine the effect of the main parameters influencing the compression stroke of a direct injection Diesel engine. The aim is to develop a methodology that can be used as a diagnostic tool to determine the compression condition of DI Diesel engines. However, conclusions derived from the present investigation can be extrapolated to other types

D. T. Hountalas; G. C. Mavropoulos; G. Kourbetis

2006-01-01

352

Chromatographic separation and characterization of mono-, di- and triaromatic hydrocarbons in gas oil  

SciTech Connect

A simple procedure is described for separation of aromatic hydrocarbons into mono-, di- and trinuclear types in Iraqi gas oil. This is accomplished by elution through an alumina adsorption column under standardized conditions. Characterization is performed by UV-absorption and ratio matching method. The method can be used also for investigating aromatic hydrocarbon structures of other petroleum fractions.

Rashid, H.A.; Fakhri, N.A.; Dekran, S.B.; Abdulla, N.I.

1989-04-01

353

Early Eocene amber from the “Pesciara di Bolca” (Lessini Mountains, Northern Italy)  

Microsoft Academic Search

The occurrence of amber from the world-famous fossil-lagerstätte of Pesciara di Bolca (Verona province) is here reported for the first time. This amber has been carefully analysed by means of infrared spectrophotometry (FTIR), thermogravimetric analysis (TG), differential thermogravimetric analysis (DTG), and elemental chemical analysis. Then, the amber from Bolca has been compared with other ambers of similar age. Among them,

Enrico Trevisani; Cesare Andrea Papazzoni; Eugenio Ragazzi; Guido Roghi

2005-01-01

354

Swirl effects on mixing and flame evolution in a research DI diesel engine  

Microsoft Academic Search

An optically accessible, DI diesel engine was used to investigate the effect of swirl on fuel-air mixing and flame evolution. Quiescent and swirling conditions were studied at three different fuel-air ratios at an engine speed of 900 RPM. For the mixing studies, performed with nitrogen to prevent combustion, a mirrored piston was used to permit double pass shadowgraph imaging within

C. Espey; J. A. Pinson; T. A. Litzinger

1990-01-01

355

oDi(pyrrole-2-carboxamides)-phenylene: pseudopolymorphs and anions recognition  

Microsoft Academic Search

o-Di-(pyrrole-2-carboxamides)-phenylene (1) was synthesized and its two pseudopolymorphs were identified. A helical assembly was observed when it was incorporated with methanol molecules via hydrogen bonding, whereas a channel assembly was formed when it was associated with DMSO molecules. The 1H NMR study revealed that 1 can recognize certain inorganic anions.

Zhenming Yin; Zucheng Li; Ao Yu; Jiaqi He; Jin-Pei Cheng

2004-01-01

356

Resistance to rice blast induced by ferric chloride, di-potassium hydrogen phosphate and salicylic acid  

Microsoft Academic Search

Ferric chloride, di-potassium hydrogen phosphate and salicylic acid were tested for their capacity to suppress rice blast under greenhouse and field conditions. In greenhouse experiments, the chemicals significantly reduced disease severity when applied as a soil drench, thus demonstrating a systemic effect. Foliar spray, but not seed treatment, also caused a reduction in blast severity. In field experiments conducted at

H. K. Manandhar; H. J. Lyngs Jørgensen; S. B. Mathur; V. Smedegaard-Petersen

1998-01-01

357

Shallow subsurface drip irrigation (S3DI) for small irregular-shaped fields in the southeast  

Technology Transfer Automated Retrieval System (TEKTRAN)

Field tests were conducted using S3DI on cotton (Gossypium hirsutum, L.), corn (Zea mays, L.), and peanut (Arachis hypogeae, L.) rotations to investigate yield potential and economic sustainability of this irrigation system. Drip tubing was installed in alternate row middles, strip tillage was used ...

358

Recent Progress in High Performance Ag-Sheathed Bi2223 Wire (DI-BSCCO®)  

NASA Astrophysics Data System (ADS)

Sumitomo Electric has been developing the silver-sheathed Bi2223 multi-filamentary wires since the discovery of Bi-based superconductors. DI-BSCCO is the high performance wires produced using the controlled-overpressure (CT-OP) sintering technique. The present commercial DI-BSCCO can provide the uniform high critical current, Ic, up to 180 A with length over 2000 m, and recently 200 A were succeeded to be obtained by the same kind of 1000 m length wires, resulting from the improvement and control of the microstructure in Bi2223 multi-filaments. The short trial wires of several meters have exhibited the highest Ic over 240 A at 77K in self-field (corresponding to 580 A per 1 cm-width). Besides, the optimization of carrier density after CT-OP led to further enhancement of Ic, reached 250 A. All the derivative products also have uniform critical current properties over entire length even after lamination with the reinforcing metals. The performances of DI-BSCCO can meet the growing needs for various application of high temperature superconductor like high in-field applications, such as magnets and motors. The recent progress in transport properties of commercial DI-BSCCO and R&D short trial wires is shown.

Kagiyama, T.; Yamazaki, K.; Kikuchi, M.; Yamade, S.; Nakashima, T.; Kobayashi, S.; Hayashi, K.; Sato, K.; Shimoyama, J.; Inoue, M.; Higashikawa, K.; Kiss, T.; Kitaguchi, H.; Kumakura, H.

2011-10-01

359

Recent R&D progress on DI-BSCCO wires with high critical current properties  

NASA Astrophysics Data System (ADS)

Sumitomo Electric has been developing the silver-sheathed Bi2223 multi-filamentary wires since the discovery of Bi-based superconductors. DI-BSCCO is the high performance wires produced with the controlled-overpressure (CT-OP) sintering technique. The present commercial DI-BSCCO can provide the uniform high critical current, Ic, up to 180 A over 2000 m, and recently 200 A were succeeded to obtain with the same kind of 1000 m-long wires, resulting from the improvement and control of the microstructure in Bi2223 multi-filaments. The short trial wires of several meter have exhibited over 240 A as the highest Ic at 77 K, self-field (corresponding to 580 A per 1 cm-width) according with high Tc over 112 K. Besides, the optimization of carrier density after CT-OP led to further enhancement of Ic, reaching 250 A. All the derivative products have also uniform critical current property over the entire length even after lamination with the reinforcements. The performances of DI-BSCCO can meet the growing needs for various high temperature superconducting applications involving high in-field properties, such as magnets and motors. The recent progress on Ic including in-field properties along with the remarkable findings on the performances of DI-BSCCO will be shown.

Nakashima, T.; Kobayashi, S.; Kagiyama, T.; Yamazaki, K.; Kikuchi, M.; Yamade, S.; Hayashi, K.; Sato, K.; Shimoyama, J.; Kitaguchi, H.; Kumakura, H.

2011-11-01

360

Characterization of Violetto di Toscana, a typical Italian variety of artichoke ( Cynara scolymus L.)  

Microsoft Academic Search

This work regards the characterization of the phenolic compounds and the mineral composition of the variety Violetto di Toscana, which is typically cultivated in Tuscany for the flavour of its edible part. All the results achieved are compared with those of var. Terom, which is widely used in Italy. A systematic study was performed on the different plant parts: the

Annalisa Romani; Patrizia Pinelli; Claudio Cantini; Antonio Cimato; Daniela Heimler

2006-01-01

361

Effects of Sewage Sludge on Di- (2-Ethylhexyl) Phthalate Uptake by Plants.  

National Technical Information Service (NTIS)

Di-(2-ethylhexyl) phthalate (DEHP) is a priority organic pollutant frequently found in municipal sludges. A greenhouse study was conducted to determine the effects of sludge on plant uptake of (14)C-DEHP (carbonyl labeled). Plants grown included three foo...

J. M. Aranda G. A. O'Connor G. A. Eiceman

1989-01-01

362

Experimental assessment of bio-reduction of Di2-thylhexyl phthalate (DEHP) under aerobic thermophilic conditions  

Microsoft Academic Search

The reduction of organic contaminants in sewage sludge is of great importance for a further sludge disposal or agricultural utilization. Laboratory scale batch experiments were performed to assess the potential use of the aerobic thermophilic treatment technique to reduce the concentration of difficult to degrade organic chemicals. Di-2-ethylhexyl phthalate (DEHP) was chosen as a model representative of these chemicals. The

Fawzi A. Banat; Stephan Prechtl; Franz Bischof

1999-01-01

363

Enzymatic acylation of di- and trisaccharides with fatty acids: choosing the appropriate enzyme, support and solvent  

Microsoft Academic Search

Enzymatic synthesis of fatty acid esters of di- and trisaccharides is limited by the fact that most biological catalysts are inactivated by the polar solvents (e.g. dimethylsulfoxide, dimethylformamide) where these carbohydrates are soluble. This article reviews the methodologies developed to overcome this limitation, namely those involving control over the reaction medium, the enzyme and the support. We have proposed the

Francisco J. Plou; M. Angeles Cruces; Manuel Ferrer; Gloria Fuentes; Eitel Pastor; Manuel Bernabé; Morten Christensen; Francisco Comelles; José L. Parra; Antonio Ballesteros

2002-01-01

364

Inhibitor of hydrogen sulfide corrosion of steel based on di- and polypropylene polyamines  

Microsoft Academic Search

Several aminoimidazolines and their derivatives are produced from di-and polypropylene polyamines and higher isomeric acids.\\u000a Varying of the composition and ratio between the components of preparative form enabled us to produce a water-soluble corrosion\\u000a inhibitor that effectively protects steel against hydrogenation and corrosion in hydrogen sulfide media.

G. I. Akhmadeeva; R. N. Zagidullin

2006-01-01

365

The Plasticizer Di(2-ethylhexyl) Phthalate Modulates ??-Aminobutyric Acid Type A and Glycine Receptor Function  

Microsoft Academic Search

INTRODUCTION: Intravenous (IV) fluid bags made of polyvinyl chloride (PVC) often contain the plasticizer di(2-ethylhexyl) phthalate (DEHP) to make the PVC flexible. Phthalate esters have been reported to inhibit neuronal nicotinic acetylcholine receptors, which are sensitive to many inhaled anesthetics. This raises the possi- bility that DEHP might modulate the function of other cys-loop receptors, such as -amino butyric acid

Liya Yang; Pavle S. Milutinovic; Robert J. Brosnan; Edmond I Eger; James M. Sonner

2007-01-01

366

The AGATA Demonstrator Array at Laboratori Nazionali di Legnaro: Status of the Project  

SciTech Connect

The AGATA Demonstrator Array is presently under installation at Laboratori Nazionali di Legnaro, Italy, where it will replace the CLARA array at the target position of the PRISMA magnetic spectrometer. In the present contribution, the details of the installation will be reviewed. Preliminary results from the first in-beam commissioning test will be given.

Farnea, E. [INFN Sezione di Padova, Padova (Italy)

2009-08-26

367

EFFECTS OF SEWAGE SLUDGE ON DI-(2-ETHYLHEXYL) PHTHALATE UPTAKE BY PLANTS  

EPA Science Inventory

Di-(2-ethylhexyl) phthalate (DEHP) is a priority organic pollutant frequently found in municipal sludges. reenhouse study was conducted to determine the effects of sludge on plant uptake of 14 C-DEHP (carbonyl labeled). lants grown included three food chain crops, lettuce (Lactuc...

368

Demanda por fundos de investimento RF e DI: um modelo de escolha discreta  

Microsoft Academic Search

Resumo : Neste artigo usamos a teoria da organização industrial para analisar os determinantes da demanda de fundos de investimento de renda fixa e DI no Brasil. Utilizamos um Modelo de Escolha Discreta conhecido como Logit Aninhado, dividindo os fundos de investimento em grupos de acordo com a similaridade das instituições que os administram. Dessa forma, evitamos os problemas relacionados

Thiago Rodrigues; Ricardo Buscariolli Pereira; Juliana Inhasz

2008-01-01

369

Suppression of histamine-induced skin reactions by loratadine and cetirizine diHCl  

Microsoft Academic Search

The skin reactivity to histamine was measured in 10 healthy and 10 atopic subjects after the ingestion of single doses of loratadine 10 mg, cetirizine diHCl 10 mg and placebo, in a double-blind cross-over randomized study.

K. Kontou-Fili; G. Paleologos; M. Herakleous

1989-01-01

370

Vehicle driving cycle performance of the spark-less di-ji hydrogen engine  

Microsoft Academic Search

The paper describes coupled CFD combustion simulations and CAE engine performance computations to describe the operation over the full range of load and speed of an always lean burn, Direct Injection Jet Ignition (DI–JI) hydrogen engine. Jet ignition pre-chambers and direct injection are enablers of high efficiencies and load control by quantity of fuel injected. Towards the end of the

Alberto A. Boretti

2010-01-01

371

SOLID SOLUTIONS OF LANTHANUM ORTHOSILICATES IN A DI-CALCIUM SILICATE  

Microsoft Academic Search

Preparation and properties of CaO-- LaâOâ - SiOâ ; orthosilicates are discussed. A limited series of solid solutions based on ; calcium orthosilicates was observed in lanthanum orthosilicate-calcium silicate ; systems. Various hightemperature di-calcium silicates are stabilized with ; transformations rates exceeding the innealing rate. (R.V.J.);

N. A. Torpov; N. F. Fedorov

1962-01-01

372

Kesan Senaman Aerobik Terhadap Penanda Stres Terpilih di Kalangan Pelajar Semasa Musim Peperiksaan Akademik  

Microsoft Academic Search

Aktiviti fizikal yang kerap seperti senaman mempunyai implikasi yang baik terhadap kehidupan seharian kita terutamanya dalam aspek kesihatan dan pengurusan stres. Kajian ini adalah bertujuan untuk mengkaji kesan program senaman aerobik dengan protokol yang ditetapkan ke atas aras penanda tekanan terpilih di kalangan pelajar universiti semasa musim peperiksaan akademik. Lapan belas (n=18) mahasiswa tidak terlatih, dipadankan dengan umur dan BMI,

FARAH FAUZI; ARIMI FITRI; MAT LUDIN; KAMARUL ZAMAN MAIDIN

2007-01-01

373

Heteroscorpionate complexes based on bis(3,5-di-tert-butylpyrazol-1-yl)dithioacetate.  

PubMed

The heteroscorpionate ligand bis(3,5-di-tert-butylpyrazol-1-yl)dithioacetate (bdtbpzdta) has been synthesized by reacting bis(3,5-di-tert-butylpyrazol-1-yl)methane with n-BuLi and CS2. The ligand was isolated as [bis(3,5-di-tert-butylpyrazol-1-yl)dithioacetato](tetrahydrofuran)lithium(I) tetrahydrofuran monosolvate, [Li(C24H39N4S2)(C4H8O)]·C4H8O or [Li(bdtbpzdta)(THF)]·THF, in which the lithium cation is bound by the ?(3)N,N',S-coordinated heteroscorpionate ligand. A similar coordination mode is observed for a zinc chloride complex bearing the bdtbpzdta ligand, namely [bis(3,5-di-tert-butylpyrazol-1-yl)dithioacetato]chloridozinc(II), [Zn(C24H39N4S2)Cl] or [Zn(bdtbpzdta)Cl], which exhibits ?(3)N,N',S-coordination and resembles the active site of zinc-containing peptide deformylases (PDFs). PMID:24005504

Tampier, Stefan; Burzlaff, Nicolai

2013-08-21

374

Isochromosome 18q in a girl with holoprosencephaly, DiGeorge anomaly, and streak ovaries.  

PubMed

We report on the clinical and pathologic findings in a girl with isochromosome 18q (46, XX,i(18q)) who had combined manifestations of monosomy 18p and trisomy 18q. Major congenital anomalies included premaxillary agenesis, alobar holoprosenphaly, double outlet right ventricle, DiGeorge anomaly and streak ovaries. The clinical spectrum in i(18q) is very broad. PMID:8368259

van Essen, A J; Schoots, C J; van Lingen, R A; Mourits, M J; Tuerlings, J H; Leegte, B

1993-08-01

375

D.O.P. and D.I. secured room (room 215), looking west towards ...  

Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

D.O.P. and D.I. secured room (room 215), looking west towards murals. Sliding map tracks are visible on the floor next to the wall at left. Also note original fluorescent lights - March Air Force Base, Strategic Air Command, Combat Operations Center, 5220 Riverside Drive, Moreno Valley, Riverside County, CA

376

DEVELOPMENTAL EXPOSURE TO DI-N-BUTYLTIN DICHLORIDE (DBTC): IMMUNOTOXIC AND NEUROTOXIC EVALUATION  

EPA Science Inventory

Organotins are incorporated as stabilizers in PVC water supply pipe. Particularly when new, mono- and di-substituted methyl- and butyltins leach from the pipe and are thus of regulatory concern to EPA. These contaminants have adverse effects on both the immune and nervous systems...

377

Study on pilot injection of DI diesel engine using common-rail injection system  

Microsoft Academic Search

In recent DI diesel engines designed to achieve high output and meet future exhaust regulation, the pilot injection control using a common-rail injection system is adopted. In this research, we developed visualization equipment for pilot combustion behavior of non-luminous flame to clarify the influence of pilot injection parameters (timing and quantity) on engine performance. As a result of this analysis,

Takehiro Tanaka; Akihiro Ando; Kazuyoshi Ishizaka

2002-01-01

378

Performing wartime memories: ceremony as contest at the Risiera di San Sabba death camp, Trieste  

Microsoft Academic Search

The paper examines contest over one of Trieste's most emotive sites of memory, the former Nazi death camp at the Risiera di San Sabba. Attention is paid to the Risiera as a site for ceremonial recollection of World War Two, and to the enmeshing of the local civic calendar of events within wider currents of academic and political revisionism affecting

Martin Purvis; David Atkinson

2009-01-01

379

Bi-modal distribution of the second messenger c-di-GMP controls cell fate and asymmetry during the caulobacter cell cycle.  

PubMed

Many bacteria mediate important life-style decisions by varying levels of the second messenger c-di-GMP. Behavioral transitions result from the coordination of complex cellular processes such as motility, surface adherence or the production of virulence factors and toxins. While the regulatory mechanisms responsible for these processes have been elucidated in some cases, the global pleiotropic effects of c-di-GMP are poorly understood, primarily because c-di-GMP networks are inherently complex in most bacteria. Moreover, the quantitative relationships between cellular c-di-GMP levels and c-di-GMP dependent phenotypes are largely unknown. Here, we dissect the c-di-GMP network of Caulobacter crescentus to establish a global and quantitative view of c-di-GMP dependent processes in this organism. A genetic approach that gradually reduced the number of diguanylate cyclases identified novel c-di-GMP dependent cellular processes and unraveled c-di-GMP as an essential component of C. crescentus cell polarity and its bimodal life cycle. By varying cellular c-di-GMP concentrations, we determined dose response curves for individual c-di-GMP-dependent processes. Relating these values to c-di-GMP levels modeled for single cells progressing through the cell cycle sets a quantitative frame for the successive activation of c-di-GMP dependent processes during the C. crescentus life cycle. By reconstructing a simplified c-di-GMP network in a strain devoid of c-di-GMP we defined the minimal requirements for the oscillation of c-di-GMP levels during the C. crescentus cell cycle. Finally, we show that although all c-di-GMP dependent cellular processes were qualitatively restored by artificially adjusting c-di-GMP levels with a heterologous diguanylate cyclase, much higher levels of the second messenger are required under these conditions as compared to the contribution of homologous c-di-GMP metabolizing enzymes. These experiments suggest that a common c-di-GMP pool cannot fully explain spatiotemporal regulation by c-di-GMP in C. crescentus and that individual enzymes preferentially regulate specific phenotypes during the cell cycle. PMID:24039597

Abel, Sören; Bucher, Tabitha; Nicollier, Micaël; Hug, Isabelle; Kaever, Volkhard; Abel Zur Wiesch, Pia; Jenal, Urs

2013-09-05

380

Atomistic simulation of lipid and DiI dynamics in membrane bilayers under tension.  

PubMed

Membrane tension modulates cellular processes by initiating changes in the dynamics of its molecular constituents. To quantify the precise relationship between tension, structural properties of the membrane, and the dynamics of lipids and a lipophilic reporter dye, we performed atomistic molecular dynamics (MD) simulations of DiI-labeled dipalmitoylphosphatidylcholine (DPPC) lipid bilayers under physiological lateral tensions ranging from -2.6 mN m(-1) to 15.9 mN m(-1). Simulations showed that the bilayer thickness decreased linearly with tension consistent with volume-incompressibility, and this thinning was facilitated by a significant increase in acyl chain interdigitation at the bilayer midplane and spreading of the acyl chains. Tension caused a significant drop in the bilayer's peak electrostatic potential, which correlated with the strong reordering of water and lipid dipoles. For the low tension regime, the DPPC lateral diffusion coefficient increased with increasing tension in accordance with free-area theory. For larger tensions, free area theory broke down due to tension-induced changes in molecular shape and friction. Simulated DiI rotational and lateral diffusion coefficients were lower than those of DPPC but increased with tension in a manner similar to DPPC. Direct correlation of membrane order and viscosity near the DiI chromophore, which was just under the DPPC headgroup, indicated that measured DiI fluorescence lifetime, which is reported to decrease with decreasing lipid order, is likely to be a good reporter of tension-induced decreases in lipid headgroup viscosity. Together, these results offer new molecular-level insights into membrane tension-related mechanotransduction and into the utility of DiI in characterizing tension-induced changes in lipid packing. PMID:21152516

Muddana, Hari S; Gullapalli, Ramachandra R; Manias, Evangelos; Butler, Peter J

2010-12-09

381

Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy  

PubMed Central

Bacteria react to adverse environmental stimuli by clustering into organized communities called biofilms. A remarkably sophisticated control system based on the dinucleotide 3?–5? cyclic diguanylic acid (c-di-GMP) is involved in deciding whether to form or abandon biofilms. The ability of c-di-GMP to form self-intercalated dimers is also thought to play a role in this complex regulation. A great advantage in the quest of elucidating the catalytic properties of the enzymes involved in c-di-GMP turnover (diguanylate cyclases and phosphodiesterases) would come from the availability of an experimental approach for in vitro quantification of c-di-GMP in real-time. Here, we show that c-di-GMP can be detected and quantified by circular dichroism (CD) spectroscopy in the low micromolar range. The method is based on the selective ability of manganese ions to induce formation of the intercalated dimer of the c-di-GMP dinucleotide in solution, which displays an intense sigmoidal CD spectrum in the near-ultraviolet region. This characteristic spectrum originates from the stacking interaction of the four mutually intercalated guanines, as it is absent in the other cyclic dinucleotide 3?–5? cyclic adenilic acid (c-di-AMP). Thus, near-ultraviolet CD can be used to effectively quantify in real-time the activity of diguanylate cyclases and phosphodiesterases in solution.

Stelitano, Valentina; Brandt, Annegret; Fernicola, Silvia; Franceschini, Stefano; Giardina, Giorgio; Pica, Andrea; Rinaldo, Serena; Sica, Filomena; Cutruzzola, Francesca

2013-01-01

382

Probing the activity of diguanylate cyclases and c-di-GMP phosphodiesterases in real-time by CD spectroscopy.  

PubMed

Bacteria react to adverse environmental stimuli by clustering into organized communities called biofilms. A remarkably sophisticated control system based on the dinucleotide 3'-5' cyclic diguanylic acid (c-di-GMP) is involved in deciding whether to form or abandon biofilms. The ability of c-di-GMP to form self-intercalated dimers is also thought to play a role in this complex regulation. A great advantage in the quest of elucidating the catalytic properties of the enzymes involved in c-di-GMP turnover (diguanylate cyclases and phosphodiesterases) would come from the availability of an experimental approach for in vitro quantification of c-di-GMP in real-time. Here, we show that c-di-GMP can be detected and quantified by circular dichroism (CD) spectroscopy in the low micromolar range. The method is based on the selective ability of manganese ions to induce formation of the intercalated dimer of the c-di-GMP dinucleotide in solution, which displays an intense sigmoidal CD spectrum in the near-ultraviolet region. This characteristic spectrum originates from the stacking interaction of the four mutually intercalated guanines, as it is absent in the other cyclic dinucleotide 3'-5' cyclic adenilic acid (c-di-AMP). Thus, near-ultraviolet CD can be used to effectively quantify in real-time the activity of diguanylate cyclases and phosphodiesterases in solution. PMID:23358823

Stelitano, Valentina; Brandt, Annegret; Fernicola, Silvia; Franceschini, Stefano; Giardina, Giorgio; Pica, Andrea; Rinaldo, Serena; Sica, Filomena; Cutruzzolà, Francesca

2013-01-28

383

Anterograde Tracing Method using DiI to Label Vagal Innervation of the Embryonic and Early Postnatal Mouse Gastrointestinal Tract  

PubMed Central

The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations, or pharmacological manipulations. Therefore, a method using 1, 1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI) was developed for labeling vagal innervation of the gastrointestinal (GI) tract in embryonic and postnatal mice. DiI labeling was adapted and optimized for this purpose by varying several facets of the method. For example, insertion and crushing of DiI crystals into the nerve led to faster DiI diffusion along vagal axons and diffusion over longer distances as compared with piercing the nerve with a micropipette tip coated with dried DiI oil. Moreover, inclusion of EDTA in the fixative reduced leakage of DiI out of nerve fibers that occurred with long incubations. Also, mounting labeled tissue in PBS was superior to glycerol with n-propyl gallate, which resulted in reduced clarity of DiI labeling that may have been due to DiI leaking out of fibers. Optical sectioning of flattened wholemounts permitted examination of individual tissue layers of the GI tract wall. This procedure aided identification of nerve ending types because in most instances each type innervates a different tissue layer. Between embryonic day 12.5 and postnatal day 8, growth of axons into the GI tract, formation and patterning of fiber bundles in the myenteric plexus and early formation of putative afferent and efferent nerve terminals were observed. Thus, the DiI tracing method developed here has opened up a window for investigation during an important phase of vagal development.

Murphy, Michelle C.; Fox, Edward A.

2007-01-01

384

Cholesterol dependent recruitment of di22:6-PC by a G protein-coupled receptor into lateral domains.  

PubMed Central

Bovine rhodopsin was reconstituted into mixtures of didocosahexaenoylphosphatidylcholine (di22:6-PC), dipalmitoylphosphatidylcholine (di16:0-PC), sn-1-palmitoyl-sn-2-docosahexaenoylphosphatidylcholine (16:0, 22:6-PC) and cholesterol. Rhodopsin denaturation was examined by using high-sensitivity differential scanning calorimetry. The unfolding temperature was increased at lower levels of lipid unsaturation, but the highest temperature was detected for native disk membranes: di22:6-PC < 16:0,22:6-PC < di16:0,18:1-PC < native disks. The incorporation of 30 mol% of cholesterol resulted in 2-4 degrees C increase of denaturation temperature in all reconstituted systems examined. From the analysis of van't Hoff's and calorimetric enthalpies, it was concluded that the presence of cholesterol in di22:6-PC-containing bilayers induces a level of cooperativity in rhodopsin unfolding. Fluorescence resonance energy transfer (FRET), using lipids labeled at the headgroup with pyrene (Py) as donors and rhodopsin retinal group as acceptor of fluorescence, was used to study rhodopsin association with lipids. Higher FRET efficiencies detected for di22:6-PE-Py, compared to di16:0-PE-Py, in mixed di22:6-PC-di16:0-PC-cholesterol bilayers, indicate preferential segregation of rhodopsin with polyunsaturated lipids. The effective range of the rhodopsin-lipid interactions facilitating cluster formation exceeds two adjacent lipid layers. In similar mixed bilayers containing no cholesterol, cluster formation was absent at temperatures above lipid phase transition, indicating a crucial role of cholesterol in microdomain formation.

Polozova, A; Litman, B J

2000-01-01

385

Cyclic di-AMP Is Critical for Listeria monocytogenes Growth, Cell Wall Homeostasis, and Establishment of Infection  

PubMed Central

ABSTRACT Listeria monocytogenes infection leads to robust induction of an innate immune signaling pathway referred to as the cytosolic surveillance pathway (CSP), characterized by expression of beta interferon (IFN-?) and coregulated genes. We previously identified the IFN-? stimulatory ligand as secreted cyclic di-AMP. Synthesis of c-di-AMP in L. monocytogenes is catalyzed by the diadenylate cyclase DacA, and multidrug resistance transporters are necessary for secretion. To identify additional bacterial factors involved in L. monocytogenes detection by the CSP, we performed a forward genetic screen for mutants that induced altered levels of IFN-?. One mutant that stimulated elevated levels of IFN-? harbored a transposon insertion in the gene lmo0052. Lmo0052, renamed here PdeA, has homology to a cyclic di-AMP phosphodiesterase, GdpP (formerly YybT), of Bacillus subtilis and is able to degrade c-di-AMP to the linear dinucleotide pApA. Reduction of c-di-AMP levels by conditional depletion of the di-adenylate cyclase DacA or overexpression of PdeA led to marked decreases in growth rates, both in vitro and in macrophages. Additionally, mutants with altered levels of c-di-AMP had different susceptibilities to peptidoglycan-targeting antibiotics, suggesting that the molecule may be involved in regulating cell wall homeostasis. During intracellular infection, increases in c-di-AMP production led to hyperactivation of the CSP. Conditional depletion of dacA also led to increased IFN-? expression and a concomitant increase in host cell pyroptosis, a result of increased bacteriolysis and subsequent bacterial DNA release. These data suggest that c-di-AMP coordinates bacterial growth, cell wall stability, and responses to stress and plays a crucial role in the establishment of bacterial infection.

Witte, Chelsea E.; Whiteley, Aaron T.; Burke, Thomas P.; Sauer, John-Demian; Portnoy, Daniel A.; Woodward, Joshua J.

2013-01-01

386

Structure of STING bound to c-di-GMP Reveals the Mechanism of Cyclic Dinucleotide Recognition by the Immune System  

PubMed Central

STING, stimulator of interferon genes, is an innate immune sensor of cyclic dinucleotides that regulates the induction of type I interferons. STING C-terminal domain forms a V-shaped dimer and binds a c-di-GMP molecule at the dimer interface through direct and solvent-mediated hydrogen bonds. The guanine bases of c-di-GMP stack against the phenolic rings of a conserved tyrosine residue. Mutations at the c-di-GMP binding surface reduce nucleotide binding and affect signaling.

Shu, Chang; Yi, Guanghui; Watts, Tylan; Kao, C. Cheng; Li, Pingwei

2012-01-01

387

N?-(3-Sulfanyl-idene-3,4-di-hydro-quinoxalin-2-yl)benzohydrazide di-methyl-formamide monosolvate  

PubMed Central

The 2-sulfanyl­idene-3,4-di­hydro­quinoxalin-2-yl ring system of the title solvate, C15H12N4OS·C3H7NO, is essentially planar, the maximum deviation from the mean plane being 0.024?(2)?Å for the thione C atom. The mean plane through the fused-ring system is almost perpendicular to the terminal phenyl ring, as indicated by the dihedral angle of 70.05?(8)°. In the crystal, the main and solvent mol­ecules are linked by N—H?O hydrogen bonds, forming a layer parallel to (010).

Zanzoul, Asmae; Essassi, El Mokhtar; Pratviel, Genevieve; Saadi, Mohamed; El Ammari, Lahcen

2013-01-01

388

Cellular Levels and Binding of c-di-GMP Control Subcellular Localization and Activity of the Vibrio cholerae Transcriptional Regulator VpsT  

PubMed Central

The second messenger, cyclic diguanylate (c-di-GMP), regulates diverse cellular processes in bacteria. C-di-GMP is produced by diguanylate cyclases (DGCs), degraded by phosphodiesterases (PDEs), and receptors couple c-di-GMP production to cellular responses. In many bacteria, including Vibrio cholerae, multiple DGCs and PDEs contribute to c-di-GMP signaling, and it is currently unclear whether the compartmentalization of c-di-GMP signaling components is required to mediate c-di-GMP signal transduction. In this study we show that the transcriptional regulator, VpsT, requires c-di-GMP binding for subcellular localization and activity. Only the additive deletion of five DGCs markedly decreases the localization of VpsT, while single deletions of each DGC do not impact VpsT localization. Moreover, mutations in residues required for c-di-GMP binding, c-di-GMP-stabilized dimerization and DNA binding of VpsT abrogate wild type localization and activity. VpsT does not co-localize or interact with DGCs suggesting that c-di-GMP from these DGCs diffuses to VpsT, supporting a model in which c-di-GMP acts at a distance. Furthermore, VpsT localization in a heterologous host, Escherichia coli, requires a catalytically active DGC and is enhanced by the presence of VpsT-target sequences. Our data show that c-di-GMP signaling can be executed through an additive cellular c-di-GMP level from multiple DGCs affecting the localization and activity of a c-di-GMP receptor and furthers our understanding of the mechanisms of second messenger signaling.

Shikuma, Nicholas J.; Fong, Jiunn C. N.; Yildiz, Fitnat H.

2012-01-01

389

Convegno. I gemelli in eta pediatrica: epidemiologia, clinica e psicologia. Istituto Superiore di Sanita. Roma, 16 novembre 2009. (Congress. Twins During Childhood: Epidemiological, Clinical and Psychological Aspects. Istituto Superiore di Sanita. Rome, November 16, 2009. Proceedings).  

National Technical Information Service (NTIS)

The Italian Twin Registry (ITR), run by the Istituto Superiore di Sanita (Italian National Institute of Health), in collaboration with the Neonatology Unit of the Policlinico Umberto I, Sapienza University of Rome, had hosted the first National Congress o...

C. Dippolito L. Penna M. Salemi

2010-01-01

390

Zn(II), Cd(II) and Cu(II) separation through organic–inorganic Hybrid Membranes containing di-(2-ethylhexyl) phosphoric acid or di-(2-ethylhexyl) dithiophosphoric acid as a carrier  

Microsoft Academic Search

A membrane process for metal recovery from aqueous solutions was studied. Metal ions diffused from the feed solution to the stripping phase through an Hybrid Membrane containing di-(2-ethylhexyl) phosphoric acid (D2EHPA) and\\/or di-(2-ethylhexyl) dithiophosphoric acid (D2EHDTPA) as a carrier. Such membranes were prepared by a sol–gel route including cellulose triacetate and polysiloxanes. Transport behaviour was evaluated for both carriers under

M. Resina; J. Macanás; J. de Gyves; M. Muñoz

2006-01-01

391

An investigation into the molecular stability of zinc di-alkyl-di-thiophosphates (ZDDPs) in relation to their use as anti-wear and anti-corrosion additives in lubricating oils  

Microsoft Academic Search

The electronic energies and structures of the monomeric, neutral and basic forms of the important lubricating oil additive zinc di-alkyl-di-thiophosphate (ZDDP) are examined by molecular optimisation techniques using semi-empirical quantum chemistry calculations (MNDO). The optimised geometries reveal good agreement with known crystallographic data. The molecular stability is found to be highest for the basic form of ZDDP. For all forms,

D. R Armstrong; E. S Ferrari; K. J Roberts; D Adams

1997-01-01

392

An examination of the reactivity of zinc di-alkyl-di-thiophosphate in relation to its use as an anti-wear and anti-corrosion additive in lubricating oils  

Microsoft Academic Search

The reactivity of the lubricating oil additive zinc di-alkyl-di-thiophosphate (ZDDP) was investigated by molecular orbital techniques. Semi-empirical quantum chemistry methods were used to model the structures of some of the complexes expected to form during the reaction of the additive with an oxygen rich steel surface. The results showed that the most stable of the complexes resulting from these reaction

D. R. Armstrong; E. S. Ferrari; K. J. Roberts; D. Adams

1998-01-01

393

Di-pi Methane-Like Photorearrangement of Dimesity(Mesitylethynyl)Borane: Synthesis, Structure, and Aromaticity of Trimesitylborirene,  

National Technical Information Service (NTIS)

The first crystalline borirene (boracyclopropene) has been prepared by the photochemically induced di-pi-methane-like rearrangement of dimesityl(mesitylethynyl) borane into trimesityl-borirene. The structure of the latter compound was confirmed by X-ray c...

J. J. Eisch B. Shafii A. L. Rheningold

1987-01-01

394

Phase Characteristics of Positional Isomers of 1,2-di(heptacosadiynoyl)-sn-glycero-3-phosphocholine; Tubule-Forming Phosphatidylcholines.  

National Technical Information Service (NTIS)

We have examined the phase behavior of positional isomers of a polymerizable diacetylenic phospholipid, 1,2-di(heptacosadiynoyl)-sn-glycero-3-phosphocholine which has the diacetylene in varying position along the acyl chains. Upon cooling multilamellar ve...

A. S. Rudolph B. P. Singh A. Singh T. G. Burke

1988-01-01

395

Solid-phase synthesis of di- and tripeptidic hydroxamic acids as inhibitors of procollagen C-proteinase.  

PubMed

A solid-phase approach to the rapid synthesis of di- and tripeptide-like hydroxamic acids is presented. These compounds are shown to be potent inhibitors of procollagen C-proteinase (PCP). PMID:11086718

Dankwardt, S M; Billedeau, R J; Lawley, L K; Abbot, S C; Martin, R L; Chan, C S; Van Wart, H E; Walker, K A

2000-11-20

396

Intercomparison of Pressure Standards between the Istituto di Metrologia 'G. Colonnetti' and the National Bureau of Standards.  

National Technical Information Service (NTIS)

Intercomparisons were performed between a primary standard gas piston gauge of the Instituto di Metrologia 'G. Colonnetti' (IMGC) and two gauges at the National Bureau of Standards. The agreement between the average pressure generated by the IMGC primary ...

J. C. Houck G. F. Molinar R. Maghenzani

1983-01-01

397

Vibrio cholerae VpsT regulates matrix production and motility by directly sensing cyclic di-GMP*  

PubMed Central

Microorganisms can switch from a planktonic, free-swimming life style to a sessile, colonial state, called a biofilm, conferring resistance to environmental stress. Conversion between the motile and biofilm life style has been attributed to increased levels of the prokaryotic second messenger cyclic di-guanosine monophosphate (c-di-GMP), yet the signaling mechanisms mediating such a global switch are poorly understood. Here we show that the transcriptional regulator VpsT from Vibrio cholerae directly senses c-di-GMP to inversely control extracellular matrix production and motility, identifying VpsT as a master regulator for biofilm formation. Rather than being regulated by phosphorylation, VpsT undergoes a change in oligomerization upon c-di-GMP binding.

Krasteva, Petya V.; Fong, Jiunn C.N.; Shikuma, Nicholas J.; Beyhan, Sinem; Navarro, Marcos V.A.S.; Yildiz, Fitnat H.; Sondermann, Holger

2010-01-01

398

Vibrio cholerae VpsT Regulates Matrix Production and Motility by Directly Sensing Cyclic di-GMP  

SciTech Connect

Microorganisms can switch from a planktonic, free-swimming life-style to a sessile, colonial state, called a biofilm, which confers resistance to environmental stress. Conversion between the motile and biofilm life-styles has been attributed to increased levels of the prokaryotic second messenger cyclic di-guanosine monophosphate (c-di-GMP), yet the signaling mechanisms mediating such a global switch are poorly understood. Here we show that the transcriptional regulator VpsT from Vibrio cholerae directly senses c-di-GMP to inversely control extracellular matrix production and motility, which identifies VpsT as a master regulator for biofilm formation. Rather than being regulated by phosphorylation, VpsT undergoes a change in oligomerization on c-di-GMP binding.

Krasteva, P.; Fong, J; Shikuma, N; Beyhan, S; Navarro, M; Yildiz, F; Sondermann, H

2010-01-01

399

Comparison of Solid Density Standards between IMGC (Istituto di Metrologia 'Gustavo Colonnetti') and NBS (National Bureau of Standards).  

National Technical Information Service (NTIS)

Solid-object density standards developed independently by the Isituto di Metrologia 'G. Colonnetti' (IMGC) and NBS, and traceable to SI units of length and mass, have been compared using a silicon transfer standard. Results agree to approximately .000001,...

A. Peuto R. S. Davis

1985-01-01

400

Linee guida per la garanzia di qualita nell'irradiazione corporea totale (Guidelines on Quality Assurance in Total Body Irradiation).  

National Technical Information Service (NTIS)

The Italian guidelines on quality assurance in Total Body Irradiation (TBI) addressing both clinical and physical-dosimetric aspects are presented. The document has been developed, with the co-ordination of the Istituto Superiore di Sanita (the Italian Na...

M. A. Tabocchini V. Viti

2002-01-01

401

First principle evaluation of the chiroptical activity of the di-phenyl-diazene derivatives  

NASA Astrophysics Data System (ADS)

Azobenzene (di-phenyl-diazene) is well-known as a photoisomerisable molecule and has been widely studied as a molecular photoswitcher. Molecular rods, where di-phenyl arms are bound to the diazene moiety, have been also synthesized. In this article we explore by first principle density functional theory calculations the chiroptical properties (electronic circular dichroism spectra, ECD) of azobenzene and its homologues with polyphenyl arms. In particular, we demonstrate that for molecules in the cis configuration the intrinsic chirality of the distorted cis diazene group dominates the ECD response, while for trans species the non-planarity of the polyphenyl arms induces peaks in the ECD spectrum. Finally, the possibility of obtaining a light-controlled chiral switch by proper functionalization is also speculated.

Benassi, Enrico; Corni, Stefano

2012-09-01

402

(Carbonato-?O,O')bis-(di-2-pyridyl-amine-?N,N')cobalt(III) bromide.  

PubMed

In the title compound, [Co(CO(3))(C(10)H(9)N(3))(2)]Br, a distorted octa-hedral coordination of the Co(III) atom is completed by four N atoms of the two chelating di-2-pyridyl-amine ligands and two O atoms of the chelating carbonate anion. The di-2-pyridyl-amine ligands are nonplanar and the dihedral angles between the 2-pyridyl groups are 29.11?(9) and 37.15?(12)°. The coordination cation, which has approximate C(2) symmetry, is connected to the bromide ion via an N-H?Br(-) hydrogen bond. The ionic pair thus formed is further assembled into a dimer via N-H?O inter-actions about an inversion centre. A set of weaker C-H?O and C-H?Br(-) inter-actions connect the dimers into a three-dimensional network. PMID:21753946

Czapik, Agnieszka; Papadopoulos, Christos; Lalia-Kantouri, Maria; Gdaniec, Maria

2011-03-09

403

Intramolecular interactions of N-(3,5-di- tert-butyl-2-hydroxyphenyl)acetamide derivatives  

NASA Astrophysics Data System (ADS)

IR-Fourier spectroscopy methods are adopted to the study of intramolecular interactions occurring in CCl4 solutions of antivirally active derivatives of N-(3,5-di- tert-butyl-2-hydroxyphenyl)acetamide. Analysis of IR spectra has shown that intramolecular H-bonds O-H···O=C and N-H···O=C are formed in solutions of these compounds. The O-H···O=C and N-H···O=C bond strengths and the direction of the equilibrium shift between the two types of conformers depend on the type of carbonyl group substituent. An intramolecular O-H···O=C H-bond is characteristic of highly active derivatives of N-(3,5-di- tert-butyl-2-hydroxyphenyl)acetamide.

Belkov, M. V.; Harbachova, A. N.; Ksendzova, G. A.; Polozov, G. I.; Skornyakov, I. V.; Sorokin, V. L.; Tolstorozhev, G. B.; Shadyro, O. I.

2010-03-01

404

New solid-state sensing materials for gaseous dimethylcadmium, di-iso-propyltelluride and dimethylmercury  

NASA Astrophysics Data System (ADS)

Simple solid-state colorimetric sensors for precursors used in and toxic by-products of metal organic vapour-phase epitaxy (MOVPE) of mercury cadmium telluride (MCT) are described. 2,2?-Bipyridine and its commercially available 4,4?-dimethyl-, 5,5?-dimethyl- and 4,4?-di-tert-butyl dialkyl derivatives have been found to change colour from white or off-white to yellow in the presence of dimethylcadmium at concentrations as low as 13 ppm. The red form of mercury(II) iodide has been tested in both hydrogen and helium gas streams and found to change colour to yellow or white in the presence of gaseous di-iso-propyltelluride at concentrations as low as 1.5 ppm. This form of mercury(II) iodide also reacts photochemically with Me2Hg in a helium or hydrogen gas stream down to a concentration of 37 ppm.

Pardoe, Jennifer A. J.; Cole-Hamilton, David. J.; Hails, Janet E.

2005-01-01

405

Prenatal diagnosis of deletion 17p13 associated with DiGeorge anomaly.  

PubMed

A fetus, subsequently shown to have the deletion 17p13, was detected at 30 weeks' gestation because of multiple anomalies and polyhydramnios on ultrasonography. The fetus died and was born at 34 weeks of gestation. Pathologic examination showed intrauterine growth retardation, double outlet right ventricle (a conotruncal cardiac defect), and thymic hypoplasia suggesting partial DiGeorge anomaly. To our knowledge, DiGeorge anomaly has not been reported previously in conjunction with del(17p) nor in the Miller-Dieker syndrome. Since this deletion is the largest deletion of distal 17p observed so far, one explanation for this association may be the presence of a gene on proximal 17p for neural crest development. PMID:3066218

Greenberg, F; Courtney, K B; Wessels, R A; Huhta, J; Carpenter, R J; Rich, D C; Ledbetter, D H

1988-09-01

406

Silicon vacancy annealing and DI luminescence in 6H-SiC  

NASA Astrophysics Data System (ADS)

Combining electron paramagnetic resonance measurements with ab initio calculations, we identify the VCCSi(SiCCSi) complex as a second annealing product of the silicon vacancy via an analysis of resolved carbon hyperfine interactions and of the zero-field splitting parameter of its excited triplet state. At high temperatures the carbon vacancy can dissociate from this complex leaving behind a diamagnetic SiC(CSi)2 complex, which is proposed to be an excellent candidate to cause the DI photoluminescence spectrum: the calculated local vibration modes fit very well with the characteristic phonon assisted structure of the photoluminescence-spectra. Furthermore, our model provides a detailed explanation for the excitonic electron-hole recombination responsible for the DI luminescence.

Pinheiro, M. V. B.; Rauls, E.; Gerstmann, U.; Greulich-Weber, S.; Overhof, H.; Spaeth, J.-M.

2004-12-01

407

Supramolecular effects on the antifungal activity of cyclodextrin/di-n-decyldimethylammonium chloride mixtures.  

PubMed

Candidiasis infections are growing problem worldwide especially for the immunocompromised individuals. Di-n-decyldimethylammonium chloride is one of the most common antifungal agents used to clean medical devices. The current study examines the antifungal mechanism of di-n-decyldimethylammonium cation and its cyclodextrin inclusion complexes. Depending on the type of cyclodextrin (?-, ?- or ?-CD), inclusion complexes can be as active as ammonium alone in terms of microorganism death (fungicidal activity). Moreover, with ?-CD inclusion complexes, synergism is observed against fungus growth (fungistatic activity). Based on molecular dynamics, we propose a mechanism supported by cell number, selective electrode and ?-potential measurements as a function of time. The mechanism involves four steps: (i) the positively-charged complex diffuses through the solution, (ii) it adsorbs onto the fungus membrane surface by electrostatic interaction, (iii) then it dissociates and the ammonium inserts in the microorganism membrane, and (iv) the change of the cell surface charge induces cell lysis. PMID:22406295

Leclercq, Loïc; Lubart, Quentin; Dewilde, Anny; Aubry, Jean-Marie; Nardello-Rataj, Véronique

2012-03-01

408

Convulsant properties of L-glutamic acid di-tert butyl ester.  

PubMed

Glutamic acid di-tert butyl ester (GTBE) was found to have a pronounced convulsant effect in mice and rats, producing recurrent clonic convulsions combined with postural and respiratory disturbances in a dosage of 0.5 mmol/kg (148 mg/kg). Tert-butyl ester derivatives of aspartic acid and alanine, and glutamic acid gamma-benzyl ester did not produce seizures. Various other glutamate esters, such as glutamic acid diethyl ester and glutamic acid dimethyl ester, have previously been found to have anticonvulsant effects, and also do not induce seizures. It is suggested that glutamic acid di-tert butyl ester may have specific pharmacological properties which differ from those of other known convulsant drugs. PMID:2863766

Freed, W J; Ghoz, E H; Crump, S

409

Comparative study of HEPA filter efficiencies when challenged with thermal- and air-jet-generated di-2-ethylhexyl sebecate, di-2-ethylhexyl phthalate, and sodium chloride  

SciTech Connect

Respirators fitted with high-efficiency particulate (HEPA) cartridge filters are designed to remove dust, fumes, mists, and airborne particulate radionuclides. If these filters are to be reused, a Quality Assurance (QA) program must be established to ensure that filter efficiency remains greater than 99.97%. The standard method for performing QA testing is to challenge the filter with a thermally generated aerosol of 0.3-..mu..m-diam di-2-ethylhexyl phthalate (DEHP). Because of potential toxicological and other problems associated with the use of monodisperse DEHP, an investigation to study measured filter efficiencies on a HEPA respirator filter population, using several recommended replacement aerosols, has been conducted. Aerosols compared in this study were thermally generated di-2-ethylhexyl sebecate (DEHS), thermally generated DEHP, air-jet-generated DEHS, and air-jet-generated salt (NaCl). The study also focused on determining compatibility for parallel use of aerosols generated for respirator-fit testing for use in QA filter testing. Results indicate that a polydisperse air-jet-generated aerosol of DEHS can substitute for thermally generated DEHP as a method of providing QA testing of HEPA respirator filters and that equipment used in the study designed for respirator quantitative-fit testing can easily be modified to perform this function. 30 refs., 28 figs., 11 tabs.

Kerschner, H.F. III; Ettinger, H.J.; DeField, J.D.; Beckman, R.

1984-12-01

410

LUMINESCENCE SPECTRA OF THE URANYL ION IN TWO GEOMETRICALLY SIMILAR COORDINATION ENVIRONMENTS: URANYL NITRATE HEXAHYDRATE AND DI-{mu}-AQUO-BIS (DIOXODINITRA-TOURANIUM(VI) DI-IMIDAZOLE  

SciTech Connect

The luminescence spectra of the uranyl ion have been obtained at room and liquid nitrogen temperatures in the crystal hosts of uranyl nitrate hexahydrate (UNH) and di~~-aquo-bis(dioxodinitratouranium(VI)) di-imidazole (UNI). In both coordination spheres, the uranyl ion lies at the center of similar, distorted coordination hexagons consisting of two bidentate nitrate groups and two water molecules; the only difference in the coordination geometries is that the water molecules are terminal in the UNH complex and bridging in the UNI complex. The uranium-uranium "bond" distance in the UNI complex is 3.93 A. At room temperature, the emission spectra of the two compounds are essentially identical, but significant differences appear upon cooling to 77°K. Vibronic structure is observed in the crystal of the UNI complex b4t not in the crystal of uranyl nitrate hexahydrate; this implies that the geom~try of the uranyl ion in the two excited states is somewhat different. An energy level sequence is presented in which the various emission lines arise from a slightly split excited state (splitting approximately 80-85 cm-1) to several vibrational levels of the ground electronic manifold. The energy spacing of the ground vibrational levels (approximately 860 cm-1) was found to vary when changing crystal systems.

Brittain, Harry G.; Perry, Dale L.

1980-02-01

411

Economics of Transition and China's Reforms Why is China di!erent from Eastern Europe? Perspectives from organization theory  

Microsoft Academic Search

We draw from recent work in organization theory to explain the di!erences in reform strategies between China and Eastern Europe. An experimentation approach was ad- opted in China whereas a big bang approach was more favored in Eastern Europe. The explanation is based on di!erences in the organizational structure of central planning: U-form in Eastern Europe and M-form in China.

Yingyi Qian; Chenggang Xu

412

1-(3,5-Di-meth-oxy-phen-yl)-4,5-dimethyl-2-phenyl-1H-imidazole  

PubMed Central

In the title mol­ecule, C19H20N2O2, the imidazole ring makes dihedral angles of 57.29?(5) and 31.54?(5)° with the attached di­meth­oxy­phenyl residue and the phenyl ring, respectively. The dihedral angle between the di­meth­oxy­phenyl and phenyl rings is 61.15?(5)°. In the crystal, pairs of C—H?N hydrogen bonds connect the mol­ecules into inversion dimers.

Divya, G.; Saravanan, K.; Santhiya, S.; Chandralekha, K.; Lakshmi, S.

2013-01-01

413

Recording ionic events from cultured, DiI-labelled myenteric neurons in the guinea-pig proximal colon  

Microsoft Academic Search

To date investigations of enteric neurons by patch clamping\\/calcium imaging have been limited by studying unidentified heterogeneous populations of neurons. In DiI-labelled colonic myenteric neurons, the feasibility of recording ionic events was determined by applying DiI either to the mucosa or the circular muscle, dispersing neurons after 48 h organotypic culture, and patch-clamping\\/calcium imaging labeled neurons after 3–7 days in

Fivos Vogalis; Kirk Hillsley; Terence Smith

2000-01-01

414

Anatomic relationships of the human nucleus of the solitary tract in the medulla oblongata: a DiI labeling study  

Microsoft Academic Search

The nucleus of the solitary tract (nTS) is a major site of brainstem control of vital functions (e.g., cardiovascular reflexes and respiration). We examined anatomic relationships of the human nucleus of the solitary tract, using a bidirectional lipophilic fluorescent tracer 1-1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI) in 10 postmortem human fetal midgestational medullae oblongatae. Labeling by diffusion of DiI from the nucleus of

Natasa Zec; Hannah C Kinney

2003-01-01

415

Anterograde tracing method using DiI to label vagal innervation of the embryonic and early postnatal mouse gastrointestinal tract  

Microsoft Academic Search

The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations or pharmacological manipulations. Therefore, a method using 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI) was developed for labeling vagal innervation of the gastrointestinal (GI) tract in embryonic and postnatal mice. DiI labeling was adapted and optimized for this purpose by varying several facets of

Michelle C. Murphy; Edward A. Fox

2007-01-01

416

Acyl chloride/DABCO-promoted acetal migration of 1,2:4,5-di-O-isopropylidene-D-fructopyranose.  

PubMed

An unprecedented acetal migration was observed when 1,2:4,5-di-O-isopropylidene-D-fructose was treated with various acyl chlorides and 1,4-diaza-bicyclo[2.2.2]octane (DABCO). 2,3:4,5-Di-O-isopropylidene-D-fructose derivatives were isolated as the only product in high to quantitative yields. The acylium cations generated in situ were speculated as the electrophilic species to initiate the migration process. PMID:17336949

Meng, Xiang-Bao; Li, Yun-Feng; Li, Zhong-Jun

2007-02-13

417

Investigation of soot formation in a D.I. diesel engine by using laser induced scattering and laser induced incandescence  

Microsoft Academic Search

Soot has a great effect on the formation of PM (Paniculate Matter) in D.I. (Direct Injection) Diesel engines. Soot in diesel\\u000a flame is formed by incomplete combustion when the fuel atomization and mixture formation were poor. Therefore, the understanding\\u000a of soot formation in a D.I. diesel engine is mandatory to reduce PM in exhaust gas. To investigate soot formation in

Ki Hyung Lee; Jae Woo Chung; Byung Soo Kim; Sang Kwon Kim

2004-01-01

418

The termini of VSV DI particle RNAs are sufficient to signal RNA encapsidation, replication, and budding to generate infectious particles  

Microsoft Academic Search

Infectious defective interfering (DI) particles of the negative-stranded RNA virus vesicular stomatitis virus (VSV) havebeen recovered from negative-sense transcripts of a plasmid that contains a full-length cDNA derived from the DI-T particle genome. In order to determine the cis-acting sequences necessary for RNA replication, encapsidation, and budding and to approximate the minimal size of RNA that can be packaged into

Asit K. Pattnaik; L. Andrew Ball; Alison Legrone; Gail W. Wertz

1995-01-01

419

Antibacterial and Physiochemical Behavior of Prepared Chitosan\\/pyridine-3,5-di-carboxylic Acid Complex for Biomedical Applications  

Microsoft Academic Search

This paper describes the preparation of a biocompatible electrostatic chitosan\\/pyridine-3,5-di-carboxylic acid (CH-PyCA) complex which is formed by the protonation (NH 3) of chitosan and deprotonation (COO) of pyridine-3,5-di-carboxylic acid in an acidic medium under mild conditions. The crystalline and structural properties of prepared CH-PyCA complex were evaluated by UV, IR XRD and H-NMR spectroscopic studies. Thermal behavior of the complex

Jay Singh; P. K. Dutta

2011-01-01

420

Characterization of Delhi iron pillar rust by X-ray diÄraction, Fourier transform infrared spectroscopy and Mossbauer spectroscopy  

Microsoft Academic Search

Rust samples obtained from the region just below the decorative bell capital of the Delhi iron pillar (DIP) have been analyzed by X-ray diÄraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Mossbauer spectroscopy. The identification of iron hydrogen phosphate hydrate in the crystalline form by XRD was unambiguous. Very weak diÄraction from the oxyhydroxides\\/oxides of iron was observed indicating that

R. Balasubramaniam; A. V. Ramesh Kumar

421

Agenesis of the Corpus Callosum Associated With DiGeorge-Velocardiofacial Syndrome: A Case Report and Review of the Literature  

Microsoft Academic Search

We report a patient with clinical and cytogenetic findings consistent with DiGeorge-velocardiofacial syndrome and agenesis of the corpus callosum. This patient represents the first report of a case of DiGeorge-velocardiofacial syndrome associated with such a central nervous system abnormality. This case, together with previous reports in the literature, suggests that structural brain abnormalities, and in particular abnormalities of the corpus

Nathan C. Kraynack; Robert W. Hostoffer; Nathaniel H. Robin

1999-01-01

422

Charge transfer complex formation between p-chloranil and 1, n-di(9-anthryl)alkanes  

Microsoft Academic Search

Dimer model compounds of polyvinylanthracenes (1,n-di(9-anthryl)alkanes, when n=1–5) were synthesized to model the effects of distance and orientation between anthracene groups in polymeric systems. Charge transfer (CT) complexes of anthracene, 9-methylanthracene and 1,n-di(9-anthryl)alkanes with p-chloranil (p-CHL) have been investigated spectrophotometrically in dichloromethane. The colored products are measured spectrophotometrically at different wavelength depending on the electronic transition between donors and acceptor.

Mustafa Arslan; John Masnovi

2006-01-01

423

Cross-National DiÄerences in Risk Preference and Lay Predictions  

Microsoft Academic Search

This research explores whether there are systematic cross-national diÄerences in choice-inferred risk preferences between Americans and Chinese. Study 1 found (a) that the Chinese were significantly more risk seeking than the Americans, yet (b) that both nationals predicted exactly the opposite — that the Americans would be more risk seeking. Study 2 compared Americans' and Chinese risk preferences in investment,

CHRISTOPHER K. HSEE; ELKE U. WEBER

424

Esperienze di diversificazione qualitativa degli alimenti: la ri-localizzazione dei circuiti produzione-consumo  

Microsoft Academic Search

Esperienze di diversificazione qualitativa degli alimenti: la ri-localizzazione dei circuiti produzione-consumo - Quality diversification in food production: initiatives of re-localisation of production-consumption networks In an era characterized by a tendency towards globalisation of food supply chains and standardization of consumption habits, many rural areas are witnessing initiatives based on the re-discovery of local food traditions and productions, and on their

Stefano Grando

2009-01-01

425

Manufacture of Fior di Latte cheese by incorporation of probiotic lactobacilli.  

PubMed

This work aimed to select heat-resistant probiotic lactobacilli to be added to Fior di Latte (high-moisture cow milk Mozzarella) cheese. First, 18 probiotic strains belonging to Lactobacillus casei, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus reuteri were screened. Resistance to heating (65 or 55°C for 10 min) varied markedly between strains. Adaptation at 42°C for 10 min increased the heat resistance at 55°C for 10 min of all probiotic lactobacilli. Heat-adapted L. delbrueckii ssp. bulgaricus SP5 (decimal reduction time at 55°C of 227.4 min) and L. paracasei BGP1 (decimal reduction time at 55°C of 40.8 min) showed the highest survival under heat conditions that mimicked the stretching of the curd and were used for the manufacture of Fior di Latte cheese. Two technology options were chosen: chemical (addition of lactic acid to milk) or biological (Streptococcus thermophilus as starter culture) acidification with or without addition of probiotics. As determined by random amplified polymorphic DNA-PCR and 16S rRNA gene analyses, the cell density of L. delbrueckii ssp. bulgaricus SP5 and L. paracasei BGP1 in chemically or biologically acidified Fior di Latte cheese was approximately 8.0 log(10)cfu/g. Microbiological, compositional, biochemical, and sensory analyses (panel test by 30 untrained judges) showed that the use of L. delbrueckii ssp. bulgaricus SP5 and L. paracasei BGP1 enhanced flavor formation and shelf-life of Fior di Latte cheeses. PMID:22281315

Minervini, F; Siragusa, S; Faccia, M; Dal Bello, F; Gobbetti, M; De Angelis, M

2012-02-01

426

Merging domino and redox chemistry: stereoselective access to di- and trisubstituted ?,?-unsaturated acids and esters.  

PubMed

Merging is the game! The coupling of a domino reaction and an internal neutral redox reaction constitutes an excellent manifold for the stereoselective synthesis of di- and trisubstituted olefins featuring a malonate unit, an ester, or a free carboxylic acid as substituents at the allylic position (see scheme; MW=microwave). The reaction utilizes simple starting materials (propargyl vinyl ethers), methanol or water as solvents, and a very simple and bench-friendly protocol. PMID:22354501

Tejedor, David; Méndez-Abt, Gabriela; Cotos, Leandro; García-Tellado, Fernando

2012-02-22

427

TO CURE E TO CARE IN SITUAZIONI DI FINE VITA: LA PROPORZIONALITÀ E LE CURE PALLIATIVE  

Microsoft Academic Search

SINTESI Per affrontare i temi bioetici inerenti alla fi ne della vita occorre mettere a fuoco la questione della proporzionalità dei trattamenti. Spesso la dimenticanza del limite intrinseco all'arte medica può sfociare in un duplice e contraddittorio atteggiamento: l'accanimento terapeutico da una lato, l'abbandono terapeutico dall'altro. L'incapacità di riconoscere nel concreto la fi nitezza porta infatti a non accettare la

Elena Colombetti

2009-01-01

428

A facile synthesis of pyrrolo-(di)-benzazocinones via an intramolecular N-acyliminium ion cyclisation.  

PubMed

A facile, moderate to high yielding synthesis of hexahydro-(di)-benzazocinones is described via an intramolecular N-acyliminium ion cyclisation. The iminium ion intermediates are formed from the readily available 4,4-diethoxybutyl amides with an excess of triflic acid. For electron-withdrawing substituents, better yields were obtained from the pre-formed 2-hydroxypyrrolidine amides. From NMR studies, at ambient temperatures the pyrrolo-benzazocin-3-ones exist as a slowly equilibrating mixture of two conformations. PMID:19081960

King, Frank D; Aliev, Abil E; Caddick, Stephen; Tocher, Derek A; Courtier-Murias, Denis

2008-11-12

429

Hydrologic and geochemical survey of the lake “Specchio di Venere” (Pantelleria island, Southern Italy)  

Microsoft Academic Search

Hydrological and geochemical studies performed on Lake Specchio di Venere on Pantelleria island (Italy) indicate that this\\u000a endorheic basin has been formed through upwelling of the water table, and that it is continuously fed by the thermal springs\\u000a situated on its shores. The lake is periodically stratified both thermally and in salinity, albeit this stratification is\\u000a rather unstable over time,

A. Aiuppa; W. D’Alessandro; S. Gurrieri; P. Madonia; F. Parello

2007-01-01

430

First Look at kT Measurements Using di-jet Correlations  

SciTech Connect

The intrinsic parton transverse momentum kT is associated to Fermi motion of the confined partons within a nucleon. In this work we concentrate effort to investigate this phenomena in di-jets simulated in the ALICE framework, for p+p collisions. The goal of this analysis is to determine the sensitivity of the observed parameters, such as, momentum imbalance and acoplanarity, on the magnitude of kT.

Dominguez, Isabel; Cuautle, Eleazar; Paic, Guy [Departamento de Fisica de Altas Energias, Instituto de Ciencias Nucleares, Universidad Nacional Autonoma de Mexico (Mexico); Diaz, Rafael [Helsinki Institute of Physics, University of Jyvaeskylae, FI-40014 Jyvaeskylae (Finland); Morsch, Andreas [CERN, PH-Division, 1211 Geneva (Switzerland)

2008-07-02

431

An Apologetic to the Neo-Pagans as Represented by Dr. Gus DiZerega  

Microsoft Academic Search

The current trend of multi-religious observance can be traced to various root sources. In particular, the advocacy of Christianity and another religious tradition is particularly strong within Neo-Pagan circles. Through historical investigation, as well as response to the writings of Dr. Gus DiZerega, a prominent Neo-Pagan proponent, these connection will be made. It will be posited that the core of

William Tennison Smitherman II

2009-01-01

432

High plasma concentrations of di-(2-ethylhexyl)-phthalate in women with endometriosis  

Microsoft Academic Search

BACKGROUND: Emerging evidence suggests a potential role for ubiquitous environmental contaminants in the physiopathology of endometriosis. Di-(2-ethylhexyl)-phthalate (DEHP), the most commonly used plasticizer in flex- ible polyvinylchloride (PVC) formulations, is a widespread environmental contaminant with potentially adverse effects on fertility in animal models. In the present study, we tested the hypothesis that DEHP and\\/or and its main metabolite, mono-ethylhexyl phthalate

L. Cobellis; G. Latini; C. DeFelice; S. Razzi; F. Ruggieri; P. Mazzeo; F. Petraglia

2003-01-01

433

Radical polymerization of di- n-butyl itaconate in the presence of Lewis acids  

Microsoft Academic Search

Intramolecular chain-transfer reaction takes place in polymerizations of itaconates at high temperatures and\\/or at low monomer concentrations. Polymerizations of di-n-butyl itaconate (DBI) were carried out at 60 °C in the presence of Lewis acids such as scandium trifluoromethansulfonate. Lewis acids hardly influenced the intramolecular chain-transfer reaction in bulk polymerizations. Polymerizations in methanol accompanied transesterification reaction catalyzed by Lewis acids. In

Tomohiro Hirano; Kousuke Higashi; Makiko Seno; Tsuneyuki Sato

2003-01-01

434

Di-?-Sulphido Complexes of Oxomolybdenum(V) with Carboxylate Ligands  

Microsoft Academic Search

In this paper we report the synthesis and study of the first examples of oxomolybdenum (V) complexes with di-?-sulphido bridges and n-pentyl, n-hexyl, n-heptyl, iso-octyl, cyclohexyl and phenyl carboxylates as ligands. The complexes have been characterized by analytical, magnetochemical, IR and visible-UV spectral methods and are assigned the formula Mo2O2S2(RC02)2.

A. L. Doadrio Villarejo; M. T. Calabuig; C. V. Ragel; A. Doadrio

1991-01-01

435

Investigating Self-Assembly and Metal Nanoclusters in Aqueous Di-Block Copolymer Solutions  

SciTech Connect

Self-assembling properties of di-block copolymers/ surfactant hybrids in aqueous solution can be exploited to obtain metal nanoparticles stable dispersion. Results will be presented here for polystyrene-block-poly(ethylene oxide) solutions. A SANS structural investigation has been performed over different molecular weights of both hydrophilic and hydrophobic block, by varying temperature and concentration of the copolymer. A SAXS characterization of micellar systems containing Pt nanoparticles is reported.

Lo Celso, F.

2002-12-01

436

Biodegradation of a Phthalate Plasticizer, Di-Isononyl Phthalate (DINP), by Sphingobium chungbukense  

Microsoft Academic Search

In this study, di-isononyl phthalate (DINP) was efficiently degraded by Sphingobium chungbukense KCTC 2955. The optimal conditions for DINP (100 mg L?1) degradation by S. chungbukense in a mineral salts medium were found to be pH 7.0, 30°C, and stirring at 200 rpm. The maximum specific rate of DINP degradation\\u000a was found to be concentration dependent, with a maximum of 4.12 mg DINP

Jae-Min Park; Miri Jeon; Eun-Suk Lim; Hyun-Ju Um; Young-Chang Kim; Jiho Min; Yang-Hoon Kim

2008-01-01

437

Molecular Characterization of Tetralogy of Fallot Within DiGeorge Critical Region of the Chromosome 22  

Microsoft Academic Search

.   The purpose of this study was to determine whether the levels of heterozygosity and microdeletion of specific loci within\\u000a the DiGeorge critical region (del22q11) are associated with different phenotypes of tetralogy of Fallot (TF). Examinations\\u000a were conducted on 84 sporadic TF patients and their unaffected parents for del22q11, using the following 9 simple tandem repeat\\u000a polymorphic microsatellite markers: D22S420,

J.-H. Lu; M.-Y. Chung; H. Betau; H.-P. Chien; J.-K. Lu

2001-01-01

438

Synthesis and electrochemical performances of di(trimethylolpropane) tetraacrylate-based gel polymer electrolyte  

Microsoft Academic Search

The di(trimethylolpropane) tetraacrylate-based gel polymer electrolyte (GPE) was stable electrochemically up to 4.5V versus Li\\/Li+ and showed the ionic conductivity of 6.2×10?3Scm?1 at room temperature. Lithium-ion polymer batteries with the GPE were prepared and their performances were also investigated. The battery showed good rate capability and low temperature performance. The cell delivered 95.1% of the initial discharge capacity at 1.0C

Hyun-Soo Kim; Seong-In Moon

2005-01-01

439

Bio-based nanocomposite coating to preserve quality of Fior di latte cheese  

Microsoft Academic Search

The aim of this study was to evaluate the effects of a bio-based coating containing silver-montmorillonite nanoparticles combined with modified-atmosphere packaging (MAP) on microbial and sensory quality decay of Fior di latte cheese. Different concentrations of silver nanoparticles (0.25, 0.50, and 1.00mg\\/mL) were dispersed in a sodium alginic acid solution (8% wt\\/vol) before coating the cheese. Modified-atmosphere packaging was made

D. Gammariello; A. Conte; G. G. Buonocore; M. A. Del Nobile

2011-01-01

440

Glutathione extraction and mass transfer in di-(2-ethylhexyl) ammonium phosphate\\/octanol reverse micelles  

Microsoft Academic Search

Di-(2-ethylhexyl) ammonium phosphate (DEHAP)\\/octanol reverse micellar extraction was demonstrated to be an effective method to separate glutathione (GSH) from yeast fermentation broth. The effect of several important factors including pH, DEHAP concentration, and types and concentration of cations was investigated, and the optimum operating conditions were obtained by orthogonal experiments, for forward extraction: pH 2.5, DEHAP concentration=0.16mol\\/L, W0=10, phase ratio

Lichun Dong; Shaojian Huang; Qiang Luo; Xiaohua Zhou; Shensheng Zheng

2009-01-01

441

Militat omnis amans: Ovidian elegy in L’incoronazione di Poppea  

Microsoft Academic Search

G.F. Busenello's libretto forL'incoronazione di Poppea, first performed in Venice in 1643 to music by Claudio Monteverdi, is based on material from Tacitus and the pseudo-SenecanOctavia. It depicts the defeat of virtue, as embodied in the figures of Seneca and Octavia, by the lust and ambition of Nero and\\u000a Poppaea Sabina. This analysis examines how the neostoic moralizing of the

Robert C. Ketterer

1998-01-01

442

Study of zinc dialkyldithiophosphate in di-ethylhexyl sebacate using electrochemical techniques  

Microsoft Academic Search

Electrochemical techniques have been applied to detect the redox behaviour of the antiwear additive zinc dithiophosphate (ZDDP)\\u000a in di-ethylhexyl sebacate (DEHS) base fluid solution and to study the interaction of ZDDP with ferrous surfaces. Using cyclic\\u000a voltammetry it has been shown that the ZDDP is oxidised in a chemically-irreversible process at applied potentials above 1.2 V\\u000a vs. Pt with an iron

X. Xu; H. Spikes

2007-01-01

443

Real time random laser properties of Rhodamine-doped di-ureasil hybrids.  

PubMed

This investigation explores, for the first time, the random laser behavior of ground powder obtained from organic-inorganic hybrid materials based on Rhodamine 6G incorporated into a di-ureasil matrix. The experimental results, both in the spectral and temporal domains, obtained by pumping with picosecond laser pulses, show the existence of efficient random laser emission in this system. Finally, the random laser performance is compared with the one of other Rhodamine-doped solid state silica compounds. PMID:20389769

Pecoraro, Edison; García-Revilla, Sara; Ferreira, Rute A S; Balda, Rolindes; Carlos, Luís D; Fernández, Joaquín

2010-03-29

444

Anatomic relationships of the human nucleus paragigantocellularis lateralis: a DiI labeling study  

Microsoft Academic Search

The nucleus paragigantocellularis lateralis (PGL) is located in the rostral ventrolateral medulla (RVLM), a brainstem region that regulates homeostatic functions, such as blood pressure and cardiovascular reflexes, respiration, central chemosensitivity and pain. In the present study, we examined anatomic relationships of the human nucleus paragigantocellularis lateralis using a bidirectional lipophilic fluorescent tracer, 1,1?-dioctadecyl-3,3,3?,3?-tetramethylindocarbocyanine perchlorate (DiI), in nine postmortem human fetal

Natasa Zec; Hannah C Kinney

2001-01-01

445

Molecular dynamics in supercooled di-isobutyl phthalate close to the glass transition  

Microsoft Academic Search

The dynamics of the alpha-relaxation in a glass-forming low-molecular-weight system, di-isobutyl phthalate (DIBP), has been studied by means of dielectric, nuclear magnetic and mechanical spectroscopies. By using these techniques we have covered a wide timescale ranging from 10-11 to 102 s. Vogel-Fulcher correlation time behaviour and the Davidson-Cole distribution function make possible a simultaneous fitting of T1, the nuclear Overhauser

W. Suchanski; S. Jurga; T. Pakula; M. Paluch; J. Ziolo

2000-01-01

446

The Antitranspirant Di1- p -menthene, a Potential Chemical Protectant of Ozone Damage to Plants  

Microsoft Academic Search

A study was conducted to evaluate the effect of pinolene-based film-forming Vapor Gard (VG) emulsion (di-1-p-menthene), a commercial antitranspirant, on the response of the sensitive bean (Phaseolus vulgaris cv. Pinto) plants to realistic ozone fumigations. Plants treated with the chemical were significantly less damaged in comparison\\u000a with untreated controls when exposed to as much as 150 ppb of ozone in the

Alessandra Francini; Giacomo Lorenzini; Cristina Nali

2011-01-01

447

Risk Reduction of Adverse Effects Due to Di(2-Ethylhexyl) Phthalate (DEHP) by Utilizing Microbial Degradation  

Microsoft Academic Search

Di-(2-ethylhexyl) phthalate (DEHP), one of the major phthalates, was reported to be a suspected endocrine-disrupting chemical (EDC) that might produce developmental or reproductive toxicities. Therefore, much effort was undertaken to reduce the potential risk of adverse effects of DEHP on humans by diminishing environmental exposure to this chemical. A bacterium was isolated from soil contaminated with DEHP at a poly(vinyl

Ji Hye Baek; Man Bock Gu; Byoung-In Sang; Seung Jun Kwack; Kyu Bong Kim; Byung Mu Lee

2009-01-01

448

Impaired metabolism of di(2?ethylhexyl) phthalate (DEHP) in old rats?an in vitro study  

Microsoft Academic Search

Liver, kidney, and lung homogenates from 45? and 630?d?old rats were incubated with 0.5 ?Ci of C?labeled di(2?ethylhexyl) phthalate (DEHP) for 40 min at 37°C. Radiochromatogram scans of the ether extracts of the incubated mixtures showed the presence of a peak corresponding to the hydrolytic product monoethylhexyl phthalate (MEHP), in addition to the parent DEHP. Preparations from old animals revealed

R. Gollamudi; H. R. Prasanna; R. Hanumantha Rao; W. H. Lawrence; J. Autian

1983-01-01

449

A mechanistic investigation of di- tert-butyl nitroxide using scanning electrochemical microscopy (SECM)  

Microsoft Academic Search

Cyclic voltammetry (CV) and scanning electrochemical microscopy (SECM) have been used to evaluate the electron-transfer mechanism of di-tert-butyl nitroxide (DTBN) in acetonitrile. The oxidation of DTBN is coupled to a rapid, irreversible chemical follow-up step that is difficult to characterize quantitatively with CV due to distortion of the voltammograms by solution resistance and mixed radial–linear diffusion within the scan rate

Lila McKay; Robert J. LeSuer

2008-01-01

450

La sezione aurea, la spirale logaritmica e i numeri di Fibonacci  

Microsoft Academic Search

\\u000a Divina proporzione. Divina perché come Dio. Come Dio una e trina, come Dio ineffabile, come Dio ubiqua. Ubiqua perché si ritrova\\u000a ovunque, nell’aria (icosaedro), nella virtù celeste (dodecaedro). Ineffabile perchè il numero che la rappresenta non può essere\\u000a pronunciato con le parole, qualunque sia il numero di cifre che si riescano a calcolare ve ne sono infinite altre ignote,\\u000a casuali,

Franco Ghione; Emilia Mezzetti; Maura Ughi

451

Sulfoxides in Julia-Lythgoe olefination: efficient and stereoselective preparation of di-, tri-, and tetrasubstituted olefins.  

PubMed

[reaction: see text] A novel modification of the classical Julia-Lythgoe olefination, using sulfoxides instead of sulfones, affords, after in situ benzoylation and SmI2/HMPA- or DMPU-mediated reductive elimination, 1,2-di-, tri-, and tetrasubstituted olefins in moderate to excellent yields and E/Z selectivity. The conditions are mild, and the procedure is broadly applicable. PMID:15932201

Pospísil, Jirí; Pospísil, Tomás; Markó, István E

2005-06-01

452

Survey of di?(2?ethylhexyl)phthalate plasticizer contamination of retail Danish milks  

Microsoft Academic Search

An investigation of residues of di?(2?ethylhexyl)?phthalate (DEHP) in retail whole milk in samples from one German and 14 Danish dairies is reported. The investigation was performed about six months after the use of DEHP?plasticized milk tubing was banned in Denmark. The results indicate a mean concentration of DEHP lower than 50 ?g\\/litre in retail whole milk. Based on these data

Jens Højslev Petersen

1991-01-01

453

Gravitational analysis of V541 Cygni, DI Herculis, and the Pioneer anomaly  

NASA Astrophysics Data System (ADS)

Detailed analyses by independent research groups over several decades reveal a significant discrepancy between the observed rate of periastron advance in the detached eclipsing binary star systems DI Herculis and V541 Cygni and the values theoretically predicted from the combined classical and general relativistic effects. A modification to Newton's gravitational theory is proposed in this investigation to account for these discrepancies, and is represented by {F} = - Gm1m2/r3{r} - Gom1m2/r2{r} where G o is a second gravitational constant. The two body problem is solved analytically in closed form, resulting in a retrograde contribution to the advance of periastron. Numerical values of G o were calculated from an analysis of the available data for each of these binary star systems, resulting in a value of G o =(1.5±0.3)×10-27 m2 kg-1 s-2 from the analysis of V541 Cygni, and Go = (1.5_{ - 1.5}^{ + 0.3}) × 10^{-27} {m}2 {kg}^{-1} {s}^{-2} from an analysis of DI Herculis. The level of agreement between these values supports the assumption that the rotational axes of the stars in V541 Cygni are oriented perpendicular to the orbital plane, as opposed to the highly inclined orbits of the stars observed in DI Herculis. An independently determined value of G o was calculated through an analysis of the Pioneer anomaly data, resulting in G o =(3.00±0.37)×10-27 m2 kg-1 s-2. Within a factor of two, this value of G o agrees with the results obtained from DI Herculis and V541 Cygni. The proposed theory predicts the "turn on" of the Pioneer anomaly to occur at a heliocentric distance of 10.0 AU, in good agreement with observation.

Reardon, Arthur C.

2011-12-01

454

Sesqui-, di-, and triterpenoids as chemosystematic markers in extant conifers—A review  

Microsoft Academic Search

Chemosystematics is a common tool in systematics and taxonomy of extant plants. Terpenoids have been found to be especially\\u000a valuable for chemosystematic investigations of conifers. A review of data in the extensive literature revealed some characteristic\\u000a distribution patterns of sesqui-, di-, and triterpenoids in extant conifer families. The numerous terpenoids can be assigned\\u000a to approximately 40 sesquiterpenoid, 17 diterpenoid, and

Angelika Otto; Volker Wilde

2001-01-01

455

Electrical and Mechanical Properties of DI-BSCCO Type HT Reinforced With Metallic Sheathes  

Microsoft Academic Search

Electrical and mechanical characteristics of Type HT tape, which is standard 4.2 mm wide DI-BSCCO tape reinforced with metallic tapes, have been evaluated. Longitudinal distributions of critical current and n-index in kilometer long Type HT tape has proved uniform from end to end just like the original insert tape, which is Type H tape. Type HT-CA reinforced with 50 mum

Naoki Ayai; Kouhei Yamazaki; Masashi Kikuchi; Goro Osabe; Hideo Takaaze; Hiromitsu Takayama; Shin-Ichi Kobayashi; Jun Fujikami; Kazuhiko Hayashi; Ken-Ichi Sato; Kozo Osamura; Hitoshi Kitaguchi; Shinji Matsumoto; Tsukasa Kiyoshi; Jun-Ichi Shimoyama

2009-01-01

456

Testicular toxicity of di-(2-ethylhexyl)phthalate in young Sprague–Dawley rats  

Microsoft Academic Search

Di-(2-ethylhexyl)phthalate (DEHP), used widely in the manufacture of plastics, is a well-known reproductive toxicant. It causes apoptosis and loss of spermatogenic cells, resulting in testicular atrophy. Reports are scarce in the literature on the progression of apoptosis following repeated doses of phthalates. DEHP's mechanism of inducing testicular atrophy has been associated with depletion of zinc in the testis. ZnT-1 is

Jung D Park; Sultan S. M Habeebu; Curtis D Klaassen

2002-01-01

457

Gene Expression Changes Induced in Mouse Liver by Di(2-ethylhexyl) Phthalate  

Microsoft Academic Search

Increasing chemical use necessitates a better understanding of how pollutants, such as di(2-ethylhexyl) phthalate (DEHP), a peroxisome proliferator and a phthalate plasticizer, affect human health. To understand the effects of DEHP exposure, we utilized microarray technology to identify novel DEHP targets in livers of male C57BL\\/6 mice treated with 1.0% dietary DEHP for 13 weeks. We identified 51 DEHP-regulated genes;

Jean S. Wong; Sarjeet S. Gill

2002-01-01

458

Di(2-ethylhexyl)phthalate (DEHP) exposure of voluntary plasma and platelet donors  

Microsoft Academic Search

Each year thousands of healthy volunteers undergo apheresis procedures to donate blood components and safe lives. However, many disposables used in apheresis contain di(2-ethylhexyl)phthalate (DEHP). This way, donors are exposed to DEHP, which is a reproductive and developmental toxicant in animals and a suspected endocrine modulator in humans.We quantified the DEHP exposure of six plasma donors, six discontinuous-flow platelet donors

Holger M. Koch; Jürgen Angerer; Hans Drexler; Reinhold Eckstein; Volker Weisbach

2005-01-01

459

Kinetics of di-(2-ethylhexyl)phthalate mineralization in sludge-amended soil  

Microsoft Academic Search

Sewage sludge is frequently used as a soil fertilizer although it may contain elevated concentrations of priority pollutants including di-(2-ethylhexyl)phthalate (DEHP). In the present study, the kinetics of microbial [[sup 14]C]DEHP mineralization was studied in laboratory microcosms with sewage sludge and agricultural soil. A biphasic model with two independent kinetic expressions was used to fit the mineralization data. The initial

Peter Lindequist Madsen; Jesper Bandsholm Thyme; Kaj Henriksen; P. Moeldrup; Peter Roslev

1999-01-01

460

Antifertility and mutagenic effects in mice from parenteral administration of di?2?ethylhexyl phthalate (DEHP)  

Microsoft Academic Search

The subcutaneous administration of 1–10 mg of undiluted di?(2?ethylhexyl)phthalate (DEHP) to adult male ICR mice on d 1, 5, and 10 was followed by mating, one to one, with untreated adult virgin females. A single mating at d 21 resulted in a reduction in the incidence of pregnancies in the DEHP?treated groups. On the other hand, repeated matings with fresh

D. K. Agarwal; W. H. Lawrence; J. Autian

1985-01-01

461

Exposure to Di(2-ethylhexyl)phthalate in Humans during Pregnancy  

Microsoft Academic Search

Background: Di(2-ethylhexyl)phthalate (DEHP), the most commonly used plasticizer, is a widespread ubiquitous environmental contaminant. The potential health hazards from exposure to DEHP and its main metabolite, mono(2-ethylhexyl)phthalate (MEHP), have been well documented. Exposure to DEHP and MEHP in humans at risk, such as pregnant women and human fetuses, has not been tested. Methods: Plasma DEHP and MEHP concentrations were measured

G. Latini; C. De Felice; G. Presta; A. Del Vecchio; F. Ruggieri; P. Mazzeo

2003-01-01

462

Ab Initio study of the di!usion mechanisms of gallium in a silicon matrix  

Microsoft Academic Search

We present the results of a study into the di!usion mechanisms of Ga defects in crystalline Si using ab initio techniques. Five stable neutral configurations for single and multi-atom defects are identified by density-functional theory (DFT) calculations within the local density approximation and using a localized basis set as implemented in the SIESTA package. Formation energy (EF) calculations on these

K. Levasseur-Smith; N. Mousseau

463

Recent Progresses of the Remote Didactic Laboratory LA.DI.RE “G. Savastano” Project  

Microsoft Academic Search

\\u000a The Remote Didactic Laboratory Laboratorio Didattico Remoto– LA.DI.RE. “G. Savastano” is an e-learning measurement laboratory supported by the Italian Ministry of University. It provides\\u000a the students of electric and electronic measurement courses at the technical university with access to remote measurement\\u000a laboratories, delivering different teaching activities related to measurement experiments. The core of the software architecture\\u000a is the integration of

P. Daponte; D. Grimaldi; S. Rapuano

464

Modeling the Heat Capacity of Spider Silk Inspired Di-block Copolymers  

Microsoft Academic Search

We synthesized and characterized a new family of di-block copolymers based on the amino acid sequences of Nephila clavipes major ampulate dragline spider silk, having the form HABn and HBAn (n=1-6), comprising an alanine-rich hydrophobic block, A, a glycine-rich hydrophilic block, B, and a histidine tag, H. Using temperature modulated differential scanning calorimetry (TMDSC), we captured the effect of bound

W. Huang; S. Krishnaji; D. Kaplan; P. Cebe

2011-01-01

465

Vegetation history and palaeoclimate of the last glacial period at Lago Grande di Monticchio, Southern Italy  

Microsoft Academic Search

A high-resolution palynological study of a 51 m core from Lago Grande di Monticchio, southern Italy, has provided a palaeonvironmental record for the last glacill. A annual lamination based chronology, supported by radiometric and tephrochronological dates, provides an absolute timescale for this record that spans 76,300 years. Correlations are established between the pollen stratigraphy, the GRIP ice core ?18O record

W. A. Watts; J. R. M. Allen; B. Huntley

1996-01-01

466

DiCoT: A Methodology for Applying Distributed Cognition to the Design of Teamworking Systems  

Microsoft Academic Search

Abstract. Distributed Cognition is growing in popularity as a way of reasoning about group working and the design of artefacts within work systems. DiCoT (Distributed Cognition for Teamwork) is a methodology and representational system we are developing to support distributed cognition analysis of small team working. It draws on ideas from Contextual Design, but re-orients them towards the principles that

Ann Blandford; Dominic Furniss

2005-01-01

467

Slope Instability Processes Affecting the Pietra Di Bismantova Geosite (Northern Apennines, Italy)  

Microsoft Academic Search

The Pietra di Bismantova, a vast biocalcarenite slab, is an impressive landmark and a unique feature in the gentle hilly landscape\\u000a of the Reggio Emilia Apennines, Italy. It consists of a stratified calcareous rock type, rich in molluscs and other fossilised\\u000a remains typical of a tropical climate. The site also has significant historical and cultural interests, including remains\\u000a from Bronze

Lisa Borgatti; Giovanni Tosatti

2010-01-01

468

Aeroelastic response of the towers for the proposed bridge over Stretto di Messina  

Microsoft Academic Search

The aeroelastic response of the 376 m high towers for the proposed bridge over Stretto di Messina was investigated through various section model tests at a geometric scale of 1:140 and on a 1:250 scale aeroelastic model tested in turbulent boundary-layer flow. This paper presents the main findings of the aeroelastic model study conducted at the Danish Maritime Institute and

G. L. Larose; M. Falco; A. Cigada

1995-01-01

469

Optimization of the LaB6 Cathode for Divertor Plasma Simulator (DiPS)  

Microsoft Academic Search

A steady-state high density LaB6 dc plasma source is developed for the divertor plasma simulator (DiPS), which is a linear device for the studies on various electric probes, plasma-wall interactions, atomic processes, and for the development of plasma diagnostics. Since the LaB6 is very weak against the thermal shock, which is possibly caused by the overflow of the discharge current

H.-J. Wool; K.-S. Chung; H.-J. You; Y.-J. Seo; G.-S. Choi; T. Lho

2006-01-01

470

Sex diÄerences in the Big Five personality factors: Testing an evolutionary hypothesis  

Microsoft Academic Search

Sex diÄerences in the Big Five personality structure, as assessed by combined JPI and PRF scales, were examined in a student population (N=528) using factor analytic and covariance structure analysis techniques. An evolutionary hypothesis was tested, that the factor which lies between classical Agreeableness vs. Hostility and Neuroticism vs. Emotional Stability is the basic dimension of dominance-related aggressiveness maintained by

Sergey V. Budaev

1999-01-01

471

Late Pleistocene coastal evolution of San Giovanni di Sinis, west Sardinia (Western Mediterranean)  

Microsoft Academic Search

The Upper Pleistocene succession of coastal deposits outcropping at San Giovanni di Sinis (west Sardinia) has been studied since the 1960s. However, the absence of consistent radiometric ages has allowed the production of competing stratigraphic subdivisions of these deposits with different Isotopic Stage (OIS\\/MIS) attributions. The succession is composed of sandy to gravelly shallow-marine, coastal aeolian and alluvial fan\\/plain deposits.

Stefano Andreucci; Vincenzo Pascucci; Andrew S. Murray; Lars B. Clemmensen

2009-01-01

472

Diatom response to mid-Holocene climate change in Lago di Massaciuccoli (Tuscany, Italy)  

Microsoft Academic Search

A sedimentary sequence from the Mediterranean coastal basin of Lago di Massaciuccoli (Tuscany, Italy) was analyzed for diatoms,\\u000a covering two periods over the past ca. 7,000 years. The site was selected because it is situated in a sensitive position at\\u000a the limit between Mediterranean and Central European climates and biomes. Our focus is on the impact of accelerated human\\u000a activity during

Aldo Marchetto; Daniele Colombaroli; Willy Tinner

2008-01-01

473

Late Pleistocene and Holocene pollen stratigraphy at Lago di Vico, central Italy  

Microsoft Academic Search

A new pollen record from Lago di Vico (core V1) provides fundamental new information towards reconstruction of flora and vegetation history in central Italy during the last 90 000 years. The chronological framework is secured by seventeen AMS14C dates, one40Ar\\/39Ar date and tephra analyses. At the base of the pollen record, i.e. shortly after the40Ar\\/39Ar date 87 000±7000 B.P., three

Donatella Magri; Laura Sadori

1999-01-01

474

A Study of Matrilineal Descent from the Perspective of the Tlingit NexA'di Eagles  

Microsoft Academic Search

The Athapaskans of the boreal forest of northwestern Canada and Alaska and the Indians of the northern Northwest Coast shared a similar social organization. It was based on the division of a group into moieties and\\/or phratries, tracing matrilineal descent, practicing exogamy, matrilocality, and sharing resources with other affiliate groups. The Sanyaqoan NexA'di Eagle clan was singular among the Tlingit

CHRIS RABICH CAMPBELL

475

Sex di¡erences in spinal excitability during observation of bipedal locomotion  

Microsoft Academic Search

This study investigated whether there are sex di¡erences in the spinal excitability of the human mirror^ neuron system. We mea- sured the modulation of spinal excitability, elicited by Ho¡mann re£ex in the left plantar £exor muscle (soleus), when women and men participants observed videos of bipedal heel-stepping (plantar dorsi£exion), standing still, and bipedal toe-stepping (plantar £exion). Men and women were

Zheng Zhou

476

Degradation of Di(2-ethylhexyl) Phthalate (DEHP) by TiO 2 Photocatalysis  

Microsoft Academic Search

The photocatalytic degradation of di-(2-ethylhexyl) phthalate (DEHP) in solution using titanium dioxide (TiO2) was analyzed in this study. It was found that DEHP was completely removed in the solution after 150 min irradiation. The\\u000a effect of different factors, such as photocatalyst amount, DEHP concentration, light intensity, pH, and temperature on photocatalytic\\u000a degradation was investigated. The degradation mechanism of DEHP with proton

Ying-Chien Chung; Chih-Yu Chen

2009-01-01

477

8 de noviembre de 2006: Mercurio pasea sobre el disco solar (Micro eclipse anular de Sol o el segundo tránsito de Mercurio del siglo XXI)  

NASA Astrophysics Data System (ADS)

On Wendsday November 8, 2006, the second transit of Mercury over the Sun of the 21st. Century, took place. In this work we present the principal characteristics of the phenomenon ocurrence, the visibility region and the local circumstances for some locations of Argentina and other countries of the world. Full TEXT IN SPANISH

Mallamaci, C. C.

478

The bradykinin B1 receptor regulates A? deposition and neuroinflammation in Tg-SwDI mice.  

PubMed

The deposition of amyloid-? peptides (A?) in the cerebral vasculature, a condition known as cerebral amyloid angiopathy, is increasingly recognized as an important component leading to intracerebral hemorrhage, neuroinflammation, and cognitive impairment in Alzheimer disease (AD) and related disorders. Recent studies demonstrated a role for the bradykinin B1 receptor (B1R) in cognitive deficits induced by A? in mice; however, its involvement in AD and cerebral amyloid angiopathy is poorly understood. Herein, we investigated the effect of B1R inhibition on AD-like neuroinflammation and amyloidosis using the transgenic mouse model (Tg-SwDI). B1R expression was found to be up-regulated in brains of Tg-SwDI mice, specifically in the vasculature, neurons, and astrocytes. Notably, administration of the B1R antagonist, R715, to 8-month-old Tg-SwDI mice for 8 weeks resulted in higher A?40 levels and increased thioflavin S-positive fibrillar A? deposition. Moreover, blockage of B1R inhibited neuroinflammation, as evidenced by the decreased accumulation of activated microglia and reactive astrocytes, diminished NF-?B activation, and reduced cytokine and chemokine levels. Together, our results indicate that B1R activation plays an important role in limiting the accumulation of A? in AD-like brain, likely through the regulation of activated glial cell accumulation and release of pro-inflammatory mediators. Therefore, the modulation of the receptor may represent a novel therapeutic approach for AD. PMID:23470163

Passos, Giselle F; Medeiros, Rodrigo; Cheng, David; Vasilevko, Vitaly; Laferla, Frank M; Cribbs, David H

2013-03-05

479

A Basic Behavior of CNG DI Combustion in a Spark-Ignited Rapid Compression Machine  

NASA Astrophysics Data System (ADS)

A basic characteristics of compressed natural gas direct-injection (CNG DI) combustion was studied by using a rapid compression machine. Results show that comparing with homogeneous mixture, CNG DI has short combustion duration, high pressure rise due to combustion, and high rate of heat release, which are considered to come from the charge stratification and the gas flow generated by the fuel injection. CNG DI can realize extremely lean combustion which reaches 0.03 equivalence ratio, ?. Combustion duration, maximum pressure rise due to combustion and combustion efficiency are found to be insensitive to the injection modes. Unburned methane showed almost the same level as that of homogeneous mixture combustion. CO increased steeply with the increase in ? when ? was greater than 0.8 due to the excessive stratification, and NOx peak value shifted to the region of lower ?. Combustion inefficiency maintains less than 0.08 in the range of ? from 0.1 to 0.9 and increases at very low ? due to bulk quenching and at higher ? due to excessive stratification. The combustion efficiency estimated from combustion products shows good agreement with that of heat release analysis.

Huang, Zuohua; Shiga, Seiichi; Ueda, Takamasa; Jingu, Nobuhisa; Nakamura, Hisao; Ishima, Tsuneaki; Obokata, Tomio; Tsue, Mitsuhiro; Kono, Michikata

480

Novel mono-, di-, and trimethylornithine membrane lipids in northern wetland planctomycetes.  

PubMed

Northern peatlands represent a significant global carbon store and commonly originate from Sphagnum moss-dominated wetlands. These ombrotrophic ecosystems are rain fed, resulting in nutrient-poor, acidic conditions. Members of the bacterial phylum Planctomycetes are highly abundant and appear to play an important role in the decomposition of Sphagnum-derived litter in these ecosystems. High-performance liquid chromatography coupled to high-resolution accurate-mass mass spectrometry (HPLC-HRAM/MS) analysis of lipid extracts of four isolated planctomycetes from wetlands of European north Russia revealed novel ornithine membrane lipids (OLs) that are mono-, di-, and trimethylated at the ?-nitrogen position of the ornithine head group. Nuclear magnetic resonance (NMR) analysis of the isolated trimethylornithine lipid confirmed the structural identification. Similar fatty acid distributions between mono-, di-, and trimethylornithine lipids suggest that the three lipid classes are biosynthetically linked, as in the sequential methylation of the terminal nitrogen in phosphatidylethanolamine to produce phosphatidylcholine. The mono-, di-, and trimethylornithine lipids described here represent the first report of methylation of the ornithine head groups in biological membranes. Various bacteria are known to produce OLs under phosphorus limitation or fatty-acid-hydroxylated OLs under thermal or acid stress. The sequential methylation of OLs, leading to a charged choline-like moiety in the trimethylornithine lipid head group, may be an adaptation to provide membrane stability under acidic conditions without the use of scarce phosphate in nutrient-poor ombrotrophic wetlands. PMID:23995937

Moore, Eli K; Hopmans, Ellen C; Rijpstra, W Irene C; Villanueva, Laura; Dedysh, Svetlana N; Kulichevskaya, Irina S; Wienk, Hans; Schoutsen, Frans; Sinninghe Damsté, Jaap S

2013-08-30

481

Di-tripeptides and oligopeptides are taken up via distinct transport mechanisms in Lactococcus lactis.  

PubMed Central

Lactococcus lactis ML3 possesses two different peptide transport systems of which the substrate size restriction and specificity have been determined. The first system is the earlier-described proton motive force-dependent di-tripeptide carrier (E. J. Smid, A. J. M. Driessen, and W. N. Konings, J. Bacteriol. 171:292-298, 1989). The second system is a metabolic energy-dependent oligopeptide transport system which transports peptides of four to at least six amino acid residues. The involvement of a specific oligopeptide transport system in the utilization of tetra-alanine and penta-alanine was established in a mutant of L. lactis MG1363 that was selected on the basis of resistance to toxic analogs of alanine and alanine-containing di- and tripeptides. This mutant is unable to transport alanine, dialanine, and trialanine but still shows uptake of tetra-alanine and penta-alanine. The oligopeptide transport system has a lower activity than the di-tripeptide transport system. Uptake of oligopeptides occurs in the absence of a proton motive force and is specifically inhibited by vanadate. The oligopeptide transport system is most likely driven by ATP or a related energy-rich, phosphorylated intermediate.

Kunji, E R; Smid, E J; Plapp, R; Poolman, B; Konings, W N

1993-01-01

482

DiGeorge Syndrome Presenting as Hypocalcaemia-Induced Seizures in Adulthood  

PubMed Central

Introduction. DiGeorge syndrome is a developmental defect commonly caused by a microdeletion on the long arm of chromosome 22 or less frequently by a deletion of the short arm of chromosome 10. Case report. We report a case of a gentleman with mild dysmorphic features who presented with hypocalcaemia-induced seizures and an associated thyroid mass with a background of learning difficulties and abnormal immune function. Discussion. DiGeorge syndrome was initially described in 1967 by Angelo DiGeorge. The majority of cases are due to a novel mutation. The resulting learning difficulties, congenital heart disease, palatal abnormalities, hypoplasia/aplasia of the parathyroid and thymus glands, and immune deficiency generally lead to diagnosis in childhood. Presentation in adulthood is rare but must be borne in mind when dealing with cases of hypocalcaemia even in the absence of florid phenotypic features. A link with malignant disease has also been reported and should lead to prompt investigation of concerning masses.

Grech Marguerat, Deborah; Psaila, Josephine; Attard, Alexander

2013-01-01

483

Myosin Light Chain Mono- and Di-Phosphorylation Differentially Regulate Adhesion and Polarity in Migrating Cells  

PubMed Central

Myosin II is a critical regulator of cell migration that generates polarity, controls protrusion, and promotes adhesion maturation and retraction of the rear. Myosin II has an ATPase motor domain that is regulated by phosphorylation of the regulatory light chain (RLC) on Thr18 and Ser19. Here, we address the activation and specific function of the two phosphorylation states of the RLC, mono- (S19) and/or di-phosphorylation (T18+S19), in cell polarity and adhesion. Specific phospho-antibodies reveal that adhesion to fibronectin via the ?5?1 integrin promotes localized mono- and di-phosphorylation of the RLC that follow different kinetics. Using phospho-mimetic mutants, we show that mono-phosphorylation promotes adhesion maturation in protrusions resulting in focal adhesions throughout the cell. In contrast, di-phosphorylation produces large, stable actomyosin bundles and large, non-dynamic adhesions that define the rear. Finally, RLC phosphorylation regulates the assembly and stability of MIIB, but not MIIA. Our data reveal a novel mechanism by which the degree of phosphorylation of the RLC differentially controls cell adhesion and polarity.

Vicente-Manzanares, Miguel; Horwitz, Alan Rick

2010-01-01

484

Cytogenetic findings in a prospective series of patients with DiGeorge anomaly.  

PubMed Central

High-resolution cytogenetics analysis of peripheral blood lymphocytes was done prospectively on 27 of 28 patients with features of DiGeorge anomaly. Twenty-two patients (81%) had normal chromosome studies with no detectable deletion in chromosome 22. Five patients (18%) had demonstrable chromosome abnormalities. Three patients had monosomy 22q11, one due to a 4q;22q translocation, one due to a 20q;22q translocation, and one due to an interstitial deletion of 22q11. One patient had monosomy 10p13, and one patient had monosomy 18q21.33, although the latter had subsequent resolution of T-cell defects. These findings are consistent with the heterogeneity of DiGeorge anomaly but confirm the association with monosomy 22q11 in some cases. However, monosomy 10p13 may also lead to this phenotype. Because of these associated chromosome findings, cytogenetic analyses should be done on patients with suspected DiGeorge anomaly. This is particularly important since many of the abnormalities involving chromosome 22 are translocations that can be familial with a higher recurrence risk. Since only one subtle, interstitial deletion of chromosome 22 was observed, it is not clear whether high-resolution cytogenetic analysis is cost beneficial for all such patients. Images Figure 1 Figure 2

Greenberg, F; Elder, F F; Haffner, P; Northrup, H; Ledbetter, D H

1988-01-01

485

Granular activated carbons from palm nut shells for gold di-cyanide adsorption  

NASA Astrophysics Data System (ADS)

Granular activated carbons were produced from palm nut shells by physical activation with steam. The proximate analysis of palm nut shells was investigated by thermogravimetric analysis, and the adsorption capacity of the activated carbons, produced as a result of shell pyrolysis at 600°C followed by steam activation at 900°C in varying activation times, was evaluated using nitrogen adsorption at 77 K. Applicability of the activated carbons for gold dicyanide adsorption was also investigated. Increasing the activation hold time with the attendant increase in the degree of carbon burn-off results in a progressive increase in the surface area of the activated carbons, reaching a value of 903.1 m2/g after activation for 6 h. The volumes of total pores, micropores, and mesopores in the activated carbons also increase progressively with the increasing degree of carbon burn-off, resulting from increasing the activation hold time. The gold di-cyanide adsorption of the activated carbons increases with the rise of pore volume of the activated carbons. The gold di-cyanide adsorption of palm nut shell activated carbon obtained after 6-h activation at 900°C is superior to that of a commercial activated carbon used for gold di-cyanide adsorption.

Buah, William K.; Williams, Paul T.

2013-02-01

486

DiGeorge Syndrome Presenting as Hypocalcaemia-Induced Seizures in Adulthood.  

PubMed

Introduction. DiGeorge syndrome is a developmental defect commonly caused by a microdeletion on the long arm of chromosome 22 or less frequently by a deletion of the short arm of chromosome 10. Case report. We report a case of a gentleman with mild dysmorphic features who presented with hypocalcaemia-induced seizures and an associated thyroid mass with a background of learning difficulties and abnormal immune function. Discussion. DiGeorge syndrome was initially described in 1967 by Angelo DiGeorge. The majority of cases are due to a novel mutation. The resulting learning difficulties, congenital heart disease, palatal abnormalities, hypoplasia/aplasia of the parathyroid and thymus glands, and immune deficiency generally lead to diagnosis in childhood. Presentation in adulthood is rare but must be borne in mind when dealing with cases of hypocalcaemia even in the absence of florid phenotypic features. A link with malignant disease has also been reported and should lead to prompt investigation of concerning masses. PMID:23762078

Zammit, Adrian; Grech Marguerat, Deborah; Psaila, Josephine; Attard, Alexander

2013-05-20

487

The bacterial second messenger c-di-GMP: Probing interactions with protein and RNA binding partners using cyclic dinucleotide analogs  

PubMed Central

The ability of bacteria to adapt to a changing environment is essential for their survival. One mechanism used to facilitate behavioral adaptations is the second messenger signaling molecule bis-(3?-5?)-cyclic dimeric guanosine monophosphate (c-di-GMP). c-di-GMP is widespread throughout the bacterial domain and plays a vital role in regulating the transition between the motile planktonic lifestyle and the sessile biofilm forming state. This second messenger also controls the virulence response of pathogenic organisms and is thought to be connected to quorum sensing, the process by which bacteria communicate with each other. The intracellular concentration of c-di-GMP is tightly regulated by the opposing enzymatic activities of diguanlyate cyclases and phosphodiesterases, which synthesize and degrade the second messenger, respectively. The change in the intracellular concentration of c-di-GMP is directly sensed by downstream targets of the second messenger, both protein and RNA, which induce the appropriate phenotypic response. This review will summarize our current state of knowledge of c-di-GMP signaling in bacteria with a focus on protein and RNA binding partners of the second messenger. Efforts towards the synthesis of c-di-GMP and its analogs are discussed as well as studies aimed at targeting these macromolecular effectors with chemically synthesized cyclic dinucleotide analogs.

Shanahan, Carly A.; Strobel, Scott A.

2013-01-01

488

A di- and tripeptide transport system can supply Listeria monocytogenes Scott A with amino acids essential for growth.  

PubMed Central

Listeria monocytogenes takes up di- and tripeptides via a proton motive force-dependent carrier protein. This peptide transport system resembles the recently cloned and sequenced secondary di- and tripeptide transport system of Lactococcus lactis (A. Hagting, E. R. S. Kunji, K. J. Leenhouts, B. Poolman, and W. N. Konings, J. Biol. Chem. 269:11391-11399, 1994). The peptide permease of L. monocytogenes has a broad substrate specificity and allows transport of the nonpeptide substrate 5-aminolevulinic acid, the toxic di- and tripeptide analogs, alanyl-beta-chloroalanine and alanyl-alanyl-beta-chloroalanine, and various di- and tripeptides. No extracellular peptide hydrolysis was detected, indicating that peptides are hydrolyzed after being transported into the cell. Indeed, peptidase activities in response to various synthetic substrates were detected in cell extracts obtained from L. monocytogenes cells grown in brain heart infusion broth or defined medium. The di- and tripeptide permease can supply L. monocytogenes with essential amino acids for growth and might contribute to growth of this pathogen in various foods where peptides are supplied by proteolytic activity of other microorganisms present in these foods. Possible roles of this di- and tripeptide transport system in the osmoregulation and virulence of L. monocytogenes are discussed.

Verheul, A; Hagting, A; Amezaga, M R; Booth, I R; Rombouts, F M; Abee, T

1995-01-01

489

The bacterial second messenger c-di-GMP: probing interactions with protein and RNA binding partners using cyclic dinucleotide analogs.  

PubMed

The ability of bacteria to adapt to a changing environment is essential for their survival. One mechanism used to facilitate behavioral adaptations is the second messenger signaling molecule bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP). c-di-GMP is widespread throughout the bacterial domain and plays a vital role in regulating the transition between the motile planktonic lifestyle and the sessile biofilm forming state. This second messenger also controls the virulence response of pathogenic organisms and is thought to be connected to quorum sensing, the process by which bacteria communicate with each other. The intracellular concentration of c-di-GMP is tightly regulated by the opposing enzymatic activities of diguanlyate cyclases and phosphodiesterases, which synthesize and degrade the second messenger, respectively. The change in the intracellular concentration of c-di-GMP is directly sensed by downstream targets of the second messenger, both protein and RNA, which induce the appropriate phenotypic response. This review will summarize our current state of knowledge of c-di-GMP signaling in bacteria with a focus on protein and RNA binding partners of the second messenger. Efforts towards the synthesis of c-di-GMP and its analogs are discussed as well as studies aimed at targeting these macromolecular effectors with chemically synthesized cyclic dinucleotide analogs. PMID:23108253

Shanahan, Carly A; Strobel, Scott A

2012-10-29

490

Two DHH Subfamily 1 Proteins in Streptococcus pneumoniae Possess Cyclic Di-AMP Phosphodiesterase Activity and Affect Bacterial Growth and Virulence.  

PubMed

Cyclic di-AMP (c-di-AMP) and cyclic di-GMP (c-di-GMP) are signaling molecules that play important roles in bacterial biology and pathogenesis. However, these nucleotides have not been explored in Streptococcus pneumoniae, an important bacterial pathogen. In this study, we characterized the c-di-AMP-associated genes of S. pneumoniae. The results showed that SPD_1392 (DacA) is a diadenylate cyclase that converts ATP to c-di-AMP. Both SPD_2032 (Pde1) and SPD_1153 (Pde2), which belong to the DHH subfamily 1 proteins, displayed c-di-AMP phosphodiesterase activity. Pde1 cleaved c-di-AMP into phosphoadenylyl adenosine (pApA), whereas Pde2 directly hydrolyzed c-di-AMP into AMP. Additionally, Pde2, but not Pde1, degraded pApA into AMP. Our results also demonstrated that both Pde1 and Pde2 played roles in bacterial growth, resistance to UV treatment, and virulence in a mouse pneumonia model. These results indicate that c-di-AMP homeostasis is essential for pneumococcal biology and disease. PMID:24013631

Bai, Yinlan; Yang, Jun; Eisele, Leslie E; Underwood, Adam J; Koestler, Benjamin J; Waters, Christopher M; Metzger, Dennis W; Bai, Guangchun

2013-09-06

491

Extraction of Palladium from Nitric Acid by Diamides of Di-picolinic Acid  

SciTech Connect

The most complicated and urgent problem of atomic industry consists in the safe isolation and storage of radioactive wastes. The long-lived radionuclides presented in high-level liquid wastes (HLLW) pose a potential threat to environment for hundreds and thousands of years. One of the possible ways to reduce the danger of HLLW storages is concerned with treatment of HLLW intended to recovery of long-lived radionuclides and their partitioning into separate fractions. The separation of the most hazardous radionuclides (like transplutonium elements (TPE)) to the individual fraction of low volume leads to decrease of the total volume of HLLW and therefore to decrease of solidified waste storage costs. It should be noted that only in the case of reprocessing it can be possible to recover individual radionuclides (or their fractions) into separate flows with further special approach to each of them. Partitioning of different HLLW is under investigation in many countries now. Numerous processes for recovery of Cs, Sr, TPE and REE have been already developed and tested. At the same time partitioning is only the first step on the road to the following synthesis of materials providing the safe storage of long-lived radionuclides over many thousands of years. The metallic palladium contained in HLLW seems to be a promising material for producing of matrices for incorporation of radioactive wastes. Different methods for palladium recovery have been investigated: reductive precipitation, electrochemical precipitation, sorption and extraction. Of prime importance are extraction methods. Phosphine oxides, carbamoyl-phosphine oxides, crown-ethers, oximes, sulfides and some other compounds were proposed as extractants towards palladium from nitric acid media. It is reasonable to recover palladium into individual fraction during waste partitioning. Diamides of malonic, di-glycolic and pyridine-dicarboxylic (di-picolinic) acids are intensively investigated as extractants for HLLW partitioning. The data on palladium extraction by amides of pyridinecarboxylic acids from HCl medium are contradictory. It was shown that ethers of pyridine-3,5-dicarboxylic acid effectively recover palladium; at the same time the data given testify that palladium is effectively extracted by diamides of malonic and di-glycolic acids, while amides of di-picolinic acid (solution of N,N'-dimethyl-N,N'-diphenyl diamide of di-picolinic acid in chloroform) do not practically extract palladium from HCl solutions. It was found in our previous works that the use of polar fluorinated diluents instead of chloroform increases the extraction ability of diamides of di-picolinic acid towards americium and europium. The main goal of the present work was to investigate the palladium extraction from nitric acid by solutions of diamides of di-picolinic acid in meta-nitro-benzo-trifluoride (F-3) under conditions that were optimal for Am recovery from HLLW. (authors)

Alyapyshev, M.Yu.; Babain, V.A.; Pokhitonov, Yu.A.; Esimantovskiy, V.M. ['V.G. Khlopin Radium Institute', 28, 2nd Mirinskiy Ave, St.-Petersburg, 194021 (Russian Federation)

2007-07-01

492

LtmA, a novel cyclic di-GMP-responsive activator, broadly regulates the expression of lipid transport and metabolism genes in Mycobacterium smegmatis  

PubMed Central

In a bis-(3?-5?)-cyclic dimeric guanosine monophosphate (c-di-GMP)/transcription factor binding screen, we identified Mycobacterium smegmatis Ms6479 as the first c-di-GMP-responsive transcriptional factor in mycobacteria. Ms6479 could specifically bind with c-di-GMP and recognize the promoters of 37 lipid transport and metabolism genes. c-di-GMP could enhance the ability of Ms6479 to bind to its target DNA. Furthermore, our results establish Ms6479 as a global activator that positively regulates the expression of diverse target genes. Overexpression of Ms6479 in M. smegmatis significantly reduced the permeability of the cell wall to crystal violet and increased mycobacterial resistance to anti-tuberculosis antibiotics. Interestingly, Ms6479 lacks the previously reported c-di-GMP binding motifs. Our findings introduce Ms6479 (here designated LtmA for lipid transport and metabolism activator) as a new c-di-GMP-responsive regulator.

Li, Weihui; He, Zheng-Guo

2012-01-01

493

Interferon induction by viruses. VIII. Vesicular stomatitis virus: (+-)DI-011 particles induce interferon in the absence of standard virions  

SciTech Connect

Evidence was presented that VSV (+-)DI-011 particles, which contain a genome of covalently linked totally self-complementary RNA, were excellent inducers of interferon (IFN) - by virtue of the dsRNA presumed to form within an infected cell, and that one molecule of that dsRNA per cell sufficed to induce a quantum yield of IFN. While the IFN-inducing capacity of (+-)DI-011 particle preparations has been confirmed, some researchers contend that DIP by themselves cannot induce IFN and that induction requires the presence of coinfecting (contaminating) standard VSV PFP. Consequently, we reexamined this question and now report that under five different conditions where the function of contaminating standard virus is reduced markedly, or eliminated, there was no diminution of the interferon-inducing particle (IFP) activity in preparations of (+-)DI-011 particles. Thus, inactivation of contaminating PFP by uv radiation or heat, the elimination (during induction) of cycling infection through the use of anti-serum (in the case of mouse L cells), and the reduction of PFP by four successive velocity sedimentation-gradient purifications had no adverse affect on the IFN-inducing capacity of DI-011 in either ''aged'' primary chick embryo cells or in mouse L(Y) cells. Furthermore, dilutions of DI-011 stocks which precluded the presence of even a single PFP still induced IFN in ''aged'' chick embryo cells. In concert these data demonstrate convincingly that IFN induction by DI-011 particles does not require coinfection with standard virus. It follows that DI-011 particles are intrinsically capable of inducing IFN.

Sekellick, M.J.; Marcus, P.I.

1982-02-01

494

Dens in Dente and Fusion Occuring in Two Supernumerary Teeth: Report of An Unusual Case ?ki Süpernümere Di?te Görülen Dens in Dente ve Füzyon: Olgu Raporu  

Microsoft Academic Search

Dens in dente ve füzyon iyi bilinen ve iyi tanimlan- mi? dental anomaliler olmasina ra?men süpernume- re di?lerde nadiren görülürler. Füzyon özellikle mak- siller anterior di?lerde olu?tu?unda estetik problem yaratir. Bu makalede, süpernumere iki anterior di?te nadir görünen dens in dente ve füzyonun birlikte görüldü?ü bir olgu rapor edilmi?tir. Although dens in dente and fusion are well-known and well-established dental

Serdar UYSAL DDS; Nihal AVCU DDS

495

Single-factor scoring validation for the Health Assessment Questionnaire-Disability Index (HAQ-DI) in patients with systemic sclerosis and comparison with early rheumatoid arthritis patients  

Microsoft Academic Search

Objective  Structural validity for the Health Assessment Questionnaire-Disability Index (HAQ-DI) has recently been provided for patients with rheumatoid arthritis (RA). The goal of the current study was to examine the structural validity of the HAQ-DI in patients with systemic sclerosis (SSc, scleroderma) and to compare its performance with that in patients with RA.Methods  The HAQ-DI structural validity was first assessed in a

Jason C. Cole; Dinesh Khanna; Philip J. Clements; James R. Seibold; Donald P. Tashkin; Harold E. Paulus; Michael R. Irwin; Sarosh J. Motivala; Daniel E. Furst

2006-01-01

496

Arabidopsis Di19 Functions as a Transcription Factor and Modulates PR1, PR2, and PR5 Expression in Response to Drought Stress.  

PubMed

The Arabidopsis Di19 (Drought-induced) gene family encodes seven Cys2/His2-type zinc-finger proteins, most with unknown functions. Here, we report that Di19 functioned as a transcriptional regulator and was involved in Arabidopsis responses to drought stress through up-regulation of pathogenesis-related PR1, PR2, and PR5 gene expressions. The Di19 T-DNA insertion mutant di19 was much more sensitive to drought stress, whereas the Di19-overexpressing lines were much more tolerant to drought stress compared with wild-type plants. Di19 exhibited transactivation activity in our yeast assay, and its transactivation activity was further confirmed in vivo. DNA-binding analysis revealed that Di19 could bind to the TACA(A/G)T element and chromatin immunoprecipitation (ChIP) assays demonstrated that Di19 could bind to the TACA(A/G)T element within the PR1, PR2, and PR5 promoters. qRT-PCR results showed that Di19 promoted the expressions of PR1, PR2, and PR5, and these heightened expressions were enhanced by CPK11, which interacted with Di19 in the nucleus. Similarly to the Di19-overexpressing line, PR1-, PR2-, and PR5-overexpressing lines also showed the drought-tolerant phenotype. The pre-treatment with salicylic acid analogs INA can enhance plants' drought tolerance. Taken together, these data demonstrate that Di19, a new type of transcription factor, directly up-regulates the expressions of PR1, PR2, and PR5 in response to drought stress, and its transactivation activity is enhanced by CPK11. PMID:23404561

Liu, Wen-Xin; Zhang, Fei-Cui; Zhang, Wen-Zheng; Song, Lian-Fen; Wu, Wei-Hua; Chen, Yi-Fang

2013-02-12

497

Comparison of the protection of ferrets against pandemic 2009 influenza A virus (H1N1) by 244 DI influenza virus and oseltamivir.  

PubMed

The main antivirals employed to combat seasonal and pandemic influenza are oseltamivir and zanamivir which act by inhibiting the virus-encoded neuraminidase. These have to be deployed close to the time of infection and antiviral resistance to the more widely used oseltamivir has arisen relatively rapidly. Defective interfering (DI) influenza virus is a natural antiviral that works in a different way to oseltamivir and za