Science.gov

Sample records for papaya tree carica

  1. How to become a tree without wood - biomechanical analysis of the stem of Carica papaya L.

    PubMed

    Kempe, A; Lautenschläger, T; Lange, A; Neinhuis, C

    2014-01-01

    Carica papaya L. does not contain wood, according to the botanical definition of wood as lignified secondary xylem. Despite its parenchymatous secondary xylem, these plants are able to grow up to 10-m high. This is surprising, as wooden structural elements are the ubiquitous strategy for supporting height growth in plants. Proposed possible alternative principles to explain the compensation for lack of wood in C. papaya are turgor pressure of the parenchyma, lignified phloem fibres in the bark, or a combination of the two. Interestingly, lignified tissue comprises only 5-8% of the entire stem mass. Furthermore, the phloem fibres do not form a compact tube enclosing the xylem, but instead form a mesh tubular structure. To investigate the mechanism of papaya's unusually high mechanical strength, a set of mechanical measurements were undertaken on whole stems and tissue sections of secondary phloem and xylem. The structural Young's modulus of mature stems reached 2.5 GPa. Since this is low compared to woody plants, the flexural rigidity of papaya stem construction may mainly be based on a higher second moment of inertia. Additionally, stem turgor pressure was determined indirectly by immersing specimens in sucrose solutions of different osmolalities, followed by mechanical tests; turgor pressure was between 0.82 and 1.25 MPa, indicating that turgor is essential for flexural rigidity of the entire stem. PMID:23656471

  2. The Crop of the DayThe Crop of the Day Papaya (Papaya (Carica papayaCarica papaya))

    E-print Network

    Gepts, Paul

    in green papaya: milky latex in green papaya: papain: proteolytic enzyme active over a broad range of pH Meat tenderizer, Clearing beer (chill-proof beer), Exfoliative cytology (stomach & intestinal cancer

  3. Spiders associated with papaya, Carica papaya L., in Puerto Rico

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The main objective of this work was to study the species composition and population dynamics of spiders associated with papaya plantings in three papaya production areas: Corozal, Isabela, and Lajas, Puerto Rico. Nineteen species representing seven families and 15 genera were identified. Members of ...

  4. Dengue fever treatment with Carica papaya leaves extracts.

    PubMed

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-08-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×10(3)/µL, 8.10×10(3)/µL, 84.0% to 55×10(3)/µL, 3.7×10(3)/µL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×10(3)/µL to 168×10(3)/µL, WBC from 3.7×10(3)/µL to 7.7×10(3)/µL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases. PMID:23569787

  5. Dengue fever treatment with Carica papaya leaves extracts

    PubMed Central

    Ahmad, Nisar; Fazal, Hina; Ayaz, Muhammad; Abbasi, Bilal Haider; Mohammad, Ijaz; Fazal, Lubna

    2011-01-01

    The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176×103/µL, 8.10×103/µL, 84.0% to 55×103/µL, 3.7×103/µL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55×103/µL to 168×103/µL, WBC from 3.7×103/µL to 7.7×103/µL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases. PMID:23569787

  6. Administration Dependent Antioxidant Effect of Carica papaya Seeds Water Extract

    PubMed Central

    Panzarini, Elisa; Dwikat, Majdi; Mariano, Stefania; Vergallo, Cristian; Dini, Luciana

    2014-01-01

    Carica papaya is widely used in folk medicine as herbal remedy to prevent, protect against, and cure several diseases. These curative properties are based on the presence in different parts of the plant of phytochemical nutrients with antioxidant effect. Seeds are the less exploited part; thus this study is aimed at assessing the antioxidant activities of the C. papaya seeds water extract against hydrogen peroxide (H2O2) oxidative stress in human skin Detroit 550 fibroblasts. C. papaya seeds water extract is not toxic and acts as a potent free radical scavenger, providing protection to Detroit 550 fibroblasts that underwent H2O2 oxidative stress. Data show that (i) the maximum protective effect is achieved by the simultaneous administration of the extract with 1?mM H2O2; (ii) the extract in presence of an oxidative stress does not increase catalase activity and prevents the release of cytochrome C and the inner mitochondrial transmembrane potential (??m) loss; (iii) the extract is more efficient than vitamin C to hamper the oxidative damage; (iv) the purified subfractions of the seeds water extract exert the same antioxidant effect of whole extract. In conclusion, C. papaya seeds water extract is potentially useful for protection against oxidative stress. PMID:24795765

  7. Cyanogenic allosides and glucosides from Passiflora edulis and Carica papaya.

    PubMed

    Seigler, David S; Pauli, Guido F; Nahrstedt, Adolf; Leen, Rosemary

    2002-08-01

    Leaf and stem material of Passiflora edulis (Passifloraceae) contains the new cyanogenic glycosides (2R)-beta-D-allopyranosyloxy-2-phenylacetonitrile (1a) and (2S)-beta-D-allopyranosyloxy-2-phenylacetonitrile (1b), along with smaller amounts of (2R)-prunasin (2a), sambunigrin (2b), and the alloside of benzyl alcohol (4); the major cyanogens of the fruits are (2R)-prunasin (2a) and (2S)-sambunigrin (2b). The major cyanogenic glycoside of Carica papaya (Caricaceae) is 2a; only small amounts of 2b also are present. We were not able to confirm the presence of a cyclopentenoid cyanogenic glycoside, tetraphyllin B, in Carica papaya leaf and stem materials. In detailed 1H NMR studies of 1a/b and 2a/b, differences in higher order effects in glucosides and allosides proved to be valuable for assignment of structures in this series. The diagnostic chemical shifts of cyanogenic methine and anomeric protons in 1a/b are sensitive to anisotropic environmental effects. The assignment of C-2 stereochemistry of 1a/b was made in analogy to previous assignments in the glucoside series and was supported by GLC analysis of the TMS ethers. PMID:12150815

  8. Crystallization and preliminary X-ray diffraction studies of the glutaminyl cyclase from Carica papaya latex

    SciTech Connect

    Azarkan, Mohamed; Clantin, Bernard; Bompard, Coralie; Belrhali, Hassan; Baeyens-Volant, Danielle; Looze, Yvan; Wintjens, René

    2005-01-01

    The glutaminyl cyclase isolated from C. papaya latex has been crystallized using the hanging-drop method. Diffraction data have been collected at ESRF beamline BM14 and processed to 1.7 Å resolution. In living systems, the intramolecular cyclization of N-terminal glutamine residues is accomplished by glutaminyl cyclase enzymes (EC 2.3.2.5). While in mammals these enzymes are involved in the synthesis of hormonal and neurotransmitter peptides, the physiological role played by the corresponding plant enzymes still remains to be unravelled. Papaya glutaminyl cyclase (PQC), a 33 kDa enzyme found in the latex of the tropical tree Carica papaya, displays an exceptional resistance to chemical and thermal denaturation as well as to proteolysis. In order to elucidate its enzymatic mechanism and to gain insights into the structural determinants underlying its remarkable stability, PQC was isolated from papaya latex, purified and crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the orthorhombic space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 62.82, b = 81.23, c = 108.17 Å and two molecules per asymmetric unit. Diffraction data have been collected at ESRF beamline BM14 and processed to a resolution of 1.7 Å.

  9. A polymorphic pseudoautosomal boundary in the Carica papaya sex chromosomes.

    PubMed

    Lappin, Fiona M; Medert, Charles M; Hawkins, Kevin K; Mardonovich, Sandra; Wu, Meng; Moore, Richard C

    2015-08-01

    Sex chromosomes are defined by a non-recombining sex-determining region (SDR) flanked by one or two pseudoautosomal regions (PARs). The genetic composition and evolutionary dynamics of the PAR is also influenced by its linkage to the differentiated non-recombining SDR; however, understanding the effects of this linkage requires a precise definition of the PAR boundary. Here, we took a molecular population genetic approach to further refine the location of the PAR boundary of the evolutionary young sex chromosomes of the tropical plant, Carica papaya. We were able to map the position of the papaya PAR boundary A to a 100-kb region between two genetic loci approximately 2 Mb upstream of the previously genetically identified PAR boundary. Furthermore, this boundary is polymorphic within natural populations of papaya, with an approximately 100-130 kb expansion of the non-recombining SDR found in 16 % of individuals surveyed. The expansion of the PAR boundary in one Y haplotype includes at least one additional gene. Homologs of this gene are involved in male gametophyte and pollen development in other plant species. PMID:25711306

  10. Influence of ripening stages on antioxidant properties of papaya fruit (Carica papaya L.)

    NASA Astrophysics Data System (ADS)

    Addai, Zuhair Radhi; Abdullah, Aminah; Mutalib, Sahilah Abd.

    2013-11-01

    Papaya (Carica papaya L. cv Eksotika) is one of the most commonly consumed tropical fruits by humans, especially Malaysians. The objective of this study was to determine the phenolic compounds and antioxidants activity in different ripening stages of papaya fruit. The fruits were harvested at five different, stages RS1, RS2, RS3, RS4, and RS5 corresponding to 12, 14, 16, 18, and 20 weeks after anthesis, respectively. Papayas fruit at five different stage of ripening were obtained from farms at Pusat Flora Cheras, JabatanPertanian and Hulu Langat Semenyih, Selangor, Malaysia. The antioxidants activity were analyzed using the total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant Power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The analyses were conducted in triplicate and the data were subjected to statistical analysis using SPSS. The results showed significant differences (P< 0.05) were found at different stages of ripening. The total phenol content TPC, TFC, FRAP and DPPH values increased significantly (P<0.05) with the ripening process. The results showed the important role of the ripening stage in increasing the antioxidant content of papaya fruits.

  11. Purification and characterization of a papaya (Carica papaya L.) pectin methylesterase isolated from a commercial papain preparation

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We purified a single stable pectin methylesterase (CpL-PME; EC 3.1.1.11) from a commercial papain preparation, which is isolated from Carica papaya (L.) fruit latex. This CpL-PME was separated from the abundant cysteine endopeptidases activities using sequential hydrophobic interaction and cation-ex...

  12. Antiplasmodial Properties and Bioassay-Guided Fractionation of Ethyl Acetate Extracts from Carica papaya Leaves

    PubMed Central

    Melariri, Paula; Campbell, William; Etusim, Paschal; Smith, Peter

    2011-01-01

    We investigated the antiplasmodial properties of crude extracts from Carica papaya leaves to trace the activity through bioassay-guided fractionation. The greatest antiplasmodial activity was observed in the ethyl acetate crude extract. C. papaya showed a high selectivity for P. falciparum against CHO cells with a selectivity index of 249.25 and 185.37 in the chloroquine-sensitive D10 and chloroquine-resistant DD2 strains, respectively. Carica papaya ethyl acetate extract was subjected to bioassay-guided fractionation to ascertain the most active fraction, which was purified and identified using high-pressure liquid chromatography (HPLC) and GC-MS (Gas chromatography-Mass spectrometry) methods. Linoleic and linolenic acids identified from the ethyl acetate fraction showed IC50 of 6.88??g/ml and 3.58??g/ml, respectively. The study demonstrated greater antiplasmodial activity of the crude ethyl acetate extract of Carica papaya leaves with an IC50 of 2.96 ± 0.14??g/ml when compared to the activity of the fractions and isolated compounds. PMID:22174990

  13. Contrasting patterns of X/Y polymorphism distinguish Carica papaya from other sex chromosome systems.

    PubMed

    Weingartner, Laura A; Moore, Richard C

    2012-12-01

    The sex chromosomes of the tropical crop papaya (Carica papaya) are evolutionarily young and consequently allow for the examination of evolutionary mechanisms that drive early sex chromosome divergence. We conducted a molecular population genetic analysis of four X/Y gene pairs from a collection of 45 wild papaya accessions. These population genetic analyses reveal striking differences in the patterns of polymorphism between the X and Y chromosomes that distinguish them from other sex chromosome systems. In most sex chromosome systems, the Y chromosome displays significantly reduced polymorphism levels, whereas the X chromosome maintains a level of polymorphism that is comparable to autosomal loci. However, the four papaya sex-linked loci that we examined display diversity patterns that are opposite this trend: the papaya X alleles exhibit significantly reduced polymorphism levels, whereas the papaya Y alleles maintain greater than expected levels of diversity. Our analyses suggest that selective sweeps in the regions of the X have contributed to this pattern while also revealing geographically restricted haplogroups on the Y. We discuss the possible role sexual selection and/or genomic conflict have played in shaping the contrasting patterns of polymorphism found for the papaya X and Y chromosomes. PMID:22855536

  14. Chemical Characterization and in Vitro Cytotoxicity on Squamous Cell Carcinoma Cells of Carica Papaya Leaf Extracts.

    PubMed

    Nguyen, Thao T; Parat, Marie-Odile; Hodson, Mark P; Pan, Jenny; Shaw, Paul N; Hewavitharana, Amitha K

    2015-01-01

    In traditional medicine, Carica papaya leaf has been used for a wide range of therapeutic applications including skin diseases and cancer. In this study, we investigated the in vitro cytotoxicity of aqueous and ethanolic extracts of Carica papaya leaves on the human oral squamous cell carcinoma SCC25 cell line in parallel with non-cancerous human keratinocyte HaCaT cells. Two out of four extracts showed a significantly selective effect towards the cancer cells and were found to contain high levels of phenolic and flavonoid compounds. The chromatographic and mass spectrometric profiles of the extracts obtained with Ultra High Performance Liquid Chromatography-Quadrupole Time of Flight-Mass Spectrometry were used to tentatively identify the bioactive compounds using comparative analysis. The principal compounds identified were flavonoids or flavonoid glycosides, particularly compounds from the kaempferol and quercetin families, of which several have previously been reported to possess anticancer activities. These results confirm that papaya leaf is a potential source of anticancer compounds and warrant further scientific investigation to validate the traditional use of papaya leaf to treat cancer. PMID:26712788

  15. Morphological study of bone marrow to assess the effects of lead acetate on haemopoiesis and aplasia and the ameliorating role of Carica papaya extract

    PubMed Central

    THAM, CHING S.; CHAKRAVARTHI, SRIKUMAR; HALEAGRAHARA, NAGARAJA; DE ALWIS, RANJIT

    2013-01-01

    Lead causes damage to the body by inducing oxidative stress. The sites of damage include the bone marrow, where marrow hypoplasia and osteosclerosis may be observed. Leaves of Carica papaya, which have antioxidant and haemopoietic properties, were tested against the effect of lead acetate in experimental rats. The rats were divided into 8 groups; control, lead acetate only, Carica papaya (50 mg and 200 mg), post-treatment with Carica papaya (50 mg and 200 mg) following lead acetate administration and pre-treatment with Carica papaya (50 mg and 200 mg) followed by lead acetate administration. The substances were administered for 14 days. The effects were evaluated by measuring protein carbonyl content (PCC) and glutathione content (GC) in the bone marrow. Histological changes in the bone marrow were also observed. The results showed that Carica papaya induced a significant reduction in the PCC activity and significantly increased the GC in the bone marrow. Carica papaya also improved the histology of the bone marrow compared with that of the lead acetate-treated group. In summary, Carica papaya was effective against the oxidative damage caused by lead acetate in the bone marrow and had a stimulatory effect on haemopoiesis. PMID:23403524

  16. In vitro erythrocyte membrane stabilization properties of Carica papaya L. leaf extracts

    PubMed Central

    Ranasinghe, Priyanga; Ranasinghe, Pathmasiri; Abeysekera, W. P. Kaushalya M.; Premakumara, G. A. Sirimal; Perera, Yashasvi S.; Gurugama, Padmalal; Gunatilake, Saman B.

    2012-01-01

    Background: Carica papaya L. fruit juice and leaf extracts are known to have many beneficial medical properties. Recent reports have claimed possible beneficial effects of C. papaya L. leaf juice in treating patients with dengue viral infections. This study aims to evaluate the membrane stabilization potential of C. papaya L. leaf extracts using an in vitro hemolytic assay. Materials and Methods: The study was conducted in between June and August 2010. Two milliliters of blood from healthy volunteers and patients with serologically confirmed current dengue infection were freshly collected and used in the assays. Fresh papaya leaves at three different maturity stages (immature, partly matured, and matured) were cleaned with distilled water, crushed, and the juice was extracted with 10 ml of cold distilled water. Freshly prepared cold water extracts of papaya leaves (1 ml containing 30 ?l of papaya leaf extracts, 20 ?l from 40% erythrocytes suspension, and 950 ?l of phosphate buffered saline) were used in the heat-induced and hypotonic-induced hemolytic assays. In dose response experiments, six different concentrations (9.375, 18.75, 37.5, 75, 150, and 300 ?g/ml) of freeze dried extracts of the partly matured leaves were used. Membrane stabilization properties were investigated with heat-induced and hypotonicity-induced hemolysis assays. Results: Extracts of papaya leaves of all three maturity levels showed a significant reduction in heat-induced hemolysis compared to controls (P < 0.05). Papaya leaf extracts of all three maturity levels showed more than 25% inhibition at a concentration of 37.5 ?g/ml. The highest inhibition of heat-induced hemolysis was observed at 37.5 ?g/ml. Inhibition activity of different maturity levels was not significantly (P < 0.05) different from one another. Heat-induced hemolysis inhibition activity did not demonstrate a linear dose response relationship. At 37.5 ?g/ml concentration of the extract, a marked inhibition of hypotonicity-induced hemolysis was observed. Conclusion: C. papaya L. leaf extracts showed a significant inhibition of hemolysis in vitro and could have a potential therapeutic effect on disease processes causing destabilization of biological membranes. PMID:23225962

  17. Development of a Gene-Centered SSR Atlas as a Resource for Papaya (Carica papaya) Marker-Assisted Selection and Population Genetic Studies

    PubMed Central

    Vidal, Newton Medeiros; Grazziotin, Ana Laura; Ramos, Helaine Christine Cancela; Pereira, Messias Gonzaga; Venancio, Thiago Motta

    2014-01-01

    Carica papaya (papaya) is an economically important tropical fruit. Molecular marker-assisted selection is an inexpensive and reliable tool that has been widely used to improve fruit quality traits and resistance against diseases. In the present study we report the development and validation of an atlas of papaya simple sequence repeat (SSR) markers. We integrated gene predictions and functional annotations to provide a gene-centered perspective for marker-assisted selection studies. Our atlas comprises 160,318 SSRs, from which 21,231 were located in genic regions (i.e. inside exons, exon-intron junctions or introns). A total of 116,453 (72.6%) of all identified repeats were successfully mapped to one of the nine papaya linkage groups. Primer pairs were designed for markers from 9,594 genes (34.5% of the papaya gene complement). Using papaya-tomato orthology assessments, we assembled a list of 300 genes (comprising 785 SSRs) potentially involved in fruit ripening. We validated our atlas by screening 73 SSR markers (including 25 fruit ripening genes), achieving 100% amplification rate and uncovering 26% polymorphism rate between the parental genotypes (Sekati and JS12). The SSR atlas presented here is the first comprehensive gene-centered collection of annotated and genome positioned papaya SSRs. These features combined with thousands of high-quality primer pairs make the atlas an important resource for the papaya research community. PMID:25393538

  18. Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.

    PubMed Central

    2014-01-01

    Background Somatic embryogenesis is a complex process regulated by numerous factors. The identification of proteins that are differentially expressed during plant development could result in the development of molecular markers of plant metabolism and provide information contributing to the monitoring and understanding of different biological responses. In addition, the identification of molecular markers could lead to the optimization of protocols allowing the use of biotechnology for papaya propagation and reproduction. This work aimed to investigate the effects of polyethylene glycol (PEG) on somatic embryo development and the protein expression profile during somatic embryo maturation in papaya (Carica papaya L.). Results The maturation treatment supplemented with 6% PEG (PEG6) resulted in the greatest number of somatic embryos and induced differential protein expression compared with cultures grown under the control treatment. Among 135 spots selected for MS/MS analysis, 76 spots were successfully identified, 38 of which were common to both treatments, while 14 spots were unique to the control treatment, and 24 spots were unique to the PEG6 treatment. The identified proteins were assigned to seven categories or were unclassified. The most representative class of proteins observed in the control treatment was associated with the stress response (25.8%), while those under PEG6 treatment were carbohydrate and energy metabolism (18.4%) and the stress response (18.4%). Conclusions The differential expression of three proteins (enolase, esterase and ADH3) induced by PEG6 treatment could play an important role in maturation, and these proteins could be characterized as candidate biomarkers of somatic embryogenesis in papaya. PMID:25076862

  19. Antioxidant activities and phenolics profiling of different parts of Carica papaya by LCMS-MS.

    PubMed

    Zunjar, V; Mammen, D; Trivedi, B M

    2015-11-01

    This article deals with the comparison of the antioxidant activity of aqueous extracts of various parts of Carica papaya L. The evaluation of total phenolic content and total flavonoid content revealed high antioxidant potential of the seeds and fruits. The free radical-scavenging potential of the aqueous extracts indicated the seeds to have better DPPH-scavenging activity than fruits. The results were augmented by the FRAP activity as well. The phenolics present in the extracts were separated and identified as 5-hydroxy feruloyl quinic acid, acetyl p-coumaryl quinic acid, quercetin-3-O-rhamnoside, syringic acid hexoside, 5-hydroxy caffeic quinic acid, peonidin-3-O-glucoside, sinapic acid-O-hexoside, cyaniding-3-O-glucose and methyl feruloyl glycoside by LCMS-MS technique. PMID:25495879

  20. Purification of a thermostable alkaline laccase from papaya (Carica papaya) using affinity chromatography.

    PubMed

    Jaiswal, Nivedita; Pandey, Veda P; Dwivedi, Upendra N

    2015-01-01

    A laccase from papaya leaves was purified to homogeneity by a two step procedure namely, heat treatment (at 70 °C) and Con-A affinity chromatography. The procedure resulted in 1386.7-fold purification of laccase with a specific activity of 41.3 units mg(-1) and an overall yield of 61.5%. The native purified laccase was found to be a hexameric protein of ? 260 kDa. The purified enzyme exhibited acidic and alkaline pH optima of 6.0 and 8.0 with the non-phenolic substrate (ABTS) and phenolic substrate (catechol), respectively. The purified laccase was found to be thermostable up to 70 °C such that it retained ? 80% activity upon 30 min incubation at 70 °C. The Arrhenius energy of activation for purified laccase was found to be 7.7 kJ mol(-1). The enzyme oxidized various phenolic and non-phenolic substrates having catalytic efficiency (K(cat)/K(m)) in the order of 7.25>0.67>0.27 mM(-1) min(-1) for ABTS, catechol and hydroquinone, respectively. The purified laccase was found to be activated by Mn(2+), Cd(2+), Ca(2+), Na(+), Fe(2+), Co(2+) and Cu(2+) while weakly inhibited by Hg(2+). The properties such as thermostability, alkaline pH optima and metal tolerance exhibited by the papaya laccase make it a promising candidate enzyme for industrial exploitation. PMID:25192855

  1. ANTIOXIDANT AND IMMUNOSTIMULANT EFFECT OF CARICA PAPAYA LINN. AQUEOUS EXTRACT IN ACRYLAMIDE INTOXICATED RATS

    PubMed Central

    Mohamed Sadek, Kadry

    2012-01-01

    Introduction: The present study was conducted to evaluate the antioxidant and immunostimulant effects of The Carica papaya fruit aqueous extract (CPF, Caricaceae) against acrylamide induced oxidative stress and improvement of Immune functions which affected by free radicals liberating acrylamide in rats. Material and methods: Sixty male wistar albino rats (195-230g) were assigned to four groups, (fifteen/group). The first group used as control group and received normal physiological saline orally daily. The second group was supplemented with acrylamide 0.05% in drinking water. The third group was gastro-gavaged with 250 mg/kg of papaya fruit extract orally on daily basis. The fourth group was supplemented with acrylamide 0.05% in drinking water and gastro-gavaged with 250 mg/kg of papaya fruit extract orally on daily basis. The chosen dose of papaya fruit extract was based on the active pharmacological dose range obtained from the orientation study earlier conducted. The experimental period was extended to forty day. At the expiration of the experimental period and night fasting, blood samples were collected from the orbital venous sinus. The sera were separated and used for determining of IgG and IgM and the stomach, liver and kidney homogenates for estimation of MDA, GSH level, SOD and CAT activity as a biomarker of lipid peroxidation and antioxidative stress. Results and discussion: The obtained results revealed that, acrylamide caused significant increases in MDA and decrease of GSH level, SOD and CAT activity due to the oxidative stress induced by acrylamide on membrane polyunsaturated fatty acids in rat’s stomach, liver and kidney while administration of CPF aqueous extract, was significantly ameliorated the increased levels of MDA and decline of GSH, SOD and CAT activity in the stomach, liver and kidney tissues caused by acrylamide toxicity. Meanwhile, CPF aqueous extract significantly increased immune functions (IgG and IgM) while acrylamide significantly decrease it specially IgG. Thus, this study suggests that acrylamide-induced oxidative stress in rats can be ameliorated by administration of CPF aqueous extract. PMID:23322975

  2. Pichia cecembensis sp. nov. isolated from a papaya fruit (Carica papaya L., Caricaceae).

    PubMed

    Bhadra, Bhaskar; Sreenivas Rao, R; Naveen Kumar, N; Chaturvedi, Preeti; Sarkar, Partha K; Shivaji, S

    2007-06-01

    The ascogenous yeast YS16T was isolated from a decaying papaya fruit. Phenotypic traits such as multilateral budding, spheroidal or elongate shape, pseudohyphae formation, asci with one or more ascospores, ability to ferment d-glucose, inability to assimilate nitrate and the presence of Q7 ubiquinone suggest its affiliation to the genus Pichia. The nearest phylogenetic neighbor, based on D1/D2 domain sequence of the 26S rRNA gene and ITS region sequence, was identified as Issatchenkia orientalis (NRRL Y-5396T, a synonym of Pichia kudriavzevii) with similarities of 98.2% and 97% respectively. In addition to the difference in the D1/D2 and ITS region sequence, YS16T differs from I. orientalis with respect to a number of phenotypic traits. However, in the phylogenetic analysis, YS16T showed close relatedness to the P. membranifaciens clade. Thus, it is proposed to assign the status of a new species to YS16T, for which the name P. cecembensis sp. nov. is proposed. The type strain of P. cecembensis sp. nov. is YS16T (=NRRL Y-27985T=JCM 13873T=CBS 10445T). PMID:17316366

  3. Lactogenic Activity of an Enzymatic Hydrolysate from Octopus vulgaris and Carica papaya in SD Rats.

    PubMed

    Cai, Bingna; Chen, Hua; Sun, Han; Sun, Huili; Wan, Peng; Chen, Deke; Pan, Jianyu

    2015-11-01

    The traditional Chinese medicine theory believes that octopus papaya soup can stimulate milk production in lactating women. The objective of this study was to determine whether dietary supplementation with an enzymatic hydrolysate of Octopus vulgaris and Carica papaya (EHOC) could increase milk production and nutritional indexes in Sprague Dawley (SD) rats. Female SD rats (n?=?24) were fed a control diet (n?=?8), EHOC-supplemented diet, or a positive control diet (Shengruzhi) from day 10 of pregnancy to day 10 of lactation. Maternal serum, mammary gland (day 10 of lactation), milk, and pup weight (daily) were collected for analysis. Results showed that the EHOC diet obviously elevated daily milk yield and pup weight compared to the control group (P?

  4. Radiations and biodegradation techniques for detoxifying Carica papaya seed oil for effective dietary and industrial use.

    PubMed

    Afolabi, Israel Sunmola; Bisi-Adeniyi, Tolulope Dorcas; Adedoyin, Toluwalase Ronke; Rotimi, Solomon Oladapo

    2015-10-01

    Benzyl isothiocyanate (BITC) is toxic in high concentration. The capacity of Aspergillus niger, microwave and ultraviolet radiations to reduce the BITC levels in Carica papaya Linn seed oil were assessed in vitro. BITC at different concentrations were periodically exposed to microwave and ultraviolet radiations for 30 min and 10 h, respectively; and to identify Aspergillus niger for 4 days. Microwave radiation significantly reduced (p?

  5. Binary Combination of Carica papaya, Areca catechu and Myristica fragrans with Piperonyl Butoxide / MGK-264 against Freshwater Snail Lymnaea acuminata.

    PubMed

    Hanif, Farhat; Singh, Dinesh Kumar

    2013-12-01

    Piperonyl butoxide (PB) and MGK-264 were used to enhance the toxicity of the active components papain, arecoline and myristicin from the plants Carica papaya, Areca catechu and Myristica fragrans, respectively, against the vector snail Lymnaea acuminata. A time- and dose-dependent relationship was observed for the toxicity of these combinations. The toxic effects of these plant-derived molluscicides in combination with the synergists PB and MGK-264 were several times higher than the effect of the individual treatments. The highest degree of synergism was observed when MGK-264 was used in combination with C. papaya latex (10.47-fold increase) and PB was used with papain (8.35-fold increase). PMID:24575245

  6. Binary Combination of Carica papaya, Areca catechu and Myristica fragrans with Piperonyl Butoxide / MGK-264 against Freshwater Snail Lymnaea acuminata

    PubMed Central

    Hanif, Farhat; Singh, Dinesh Kumar

    2013-01-01

    Piperonyl butoxide (PB) and MGK-264 were used to enhance the toxicity of the active components papain, arecoline and myristicin from the plants Carica papaya, Areca catechu and Myristica fragrans, respectively, against the vector snail Lymnaea acuminata. A time- and dose-dependent relationship was observed for the toxicity of these combinations. The toxic effects of these plant-derived molluscicides in combination with the synergists PB and MGK-264 were several times higher than the effect of the individual treatments. The highest degree of synergism was observed when MGK-264 was used in combination with C. papaya latex (10.47-fold increase) and PB was used with papain (8.35-fold increase). PMID:24575245

  7. Low-dose gamma irradiation following hot water immersion of papaya (Carica papaya linn.) fruits provides additional control of postharvest fungal infection to extend shelf life

    NASA Astrophysics Data System (ADS)

    Rashid, M. H. A.; Grout, B. W. W.; Continella, A.; Mahmud, T. M. M.

    2015-05-01

    Low-dose gamma irradiation (0.08 kGy over 10 min), a level significantly below that required to satisfy the majority of international quarantine regulations, has been employed to provide a significant reduction in visible fungal infection on papaya fruit surfaces. This is appropriate for local and national markets in producer countries where levels of commercial acceptability can be retained despite surface lesions due to fungal infection. Irradiation alone and in combination with hot-water immersion (50 °C for 10 min) has been applied to papaya (Carica papaya L.) fruits at both the mature green and 1/3 yellow stages of maturity. The incidence and severity of surface fungal infections, including anthracnose, were significantly reduced by the combined treatment compared to irradiation or hot water treatment alone, extending storage at 11 °C by 13 days and retaining commercial acceptability. The combined treatment had no significant, negative impact on ripening, with quality characteristics such as surface and internal colour change, firmness, soluble solids, acidity and vitamin C maintained at acceptable levels.

  8. Effect of papaya trunk angle on infestation by white peach scale, Pseudaulacaspis pentagona (Hemiptera: Diaspididae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two papaya (Carica papaya L.) seedlings growing in one planting hole often results in angular or non-vertical growth of the trees. Data on trunk angularity, or leaning, (deviation from the vertical line of reference) and white peach scale, Pseudaulacaspis pentagona Targioni-Tozzetti (Hemiptera: Dias...

  9. Physico-chemical characteristics of papaya (Carica papaya L.) seed oil of the Hong Kong/Sekaki variety.

    PubMed

    Yanty, Noorzianna Abdul Manaf; Marikkar, Jalaldeen Mohammed Nazrim; Nusantoro, Bangun Prajanto; Long, Kamariah; Ghazali, Hasanah Mohd

    2014-01-01

    A study was carried out to determine the physicochemical characteristics of the oil derived from papaya seeds of the Hong Kong/Sekaki variety. Proximate analysis showed that seeds of the Hong Kong/Sekaki variety contained considerable amount of oil (27.0%). The iodine value, saponification value, unsaponifiable matter and free fatty acid contents of freshly extracted papaya seed oil were 76.9 g I2/100g oil, 193.5 mg KOH/g oil, 1.52% and 0.91%, respectively. The oil had a Lovibond color index of 15.2Y + 5.2B. Papaya seed oil contained ten detectable fatty acids, of which 78.33% were unsaturated. Oleic (73.5%) acid was the dominant fatty acids followed by palmitic acid (15.8%). Based on the high performance liquid chromatography (HPLC) analysis, seven species of triacylglycerols (TAGs) were detected. The predominant TAGs of papaya seed oil were OOO (40.4%), POO (29.1%) and SOO (9.9%) where O, P, and S denote oleic, palmitic and stearic acids, respectively. Thermal analysis by differential scanning calorimetry (DSC) showed that papaya seed oil had its major melting and crystallization transitions at 12.4°C and -48.2°C, respectively. Analysis of the sample by Z-nose (electronic nose) instrument showed that the sample had a high level of volatile compounds. PMID:25174674

  10. Papaya

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya is a tropical crop that is widely grown in household gardens and also commercially in plantations. However, papaya production has been severely limited by papaya ringspot virus (PRSV), the most widespread and damaging virus that affects papaya. In Hawaii, PRSV had been present since the 194...

  11. Safety Evaluation of Oral Toxicity of Carica papaya Linn. Leaves: A Subchronic Toxicity Study in Sprague Dawley Rats

    PubMed Central

    Ismail, Zakiah; Abdullah, Noor Rain; Abdul Rashid, Badrul Amini; Jantan, Ibrahim

    2014-01-01

    The subchronic toxicity effect of the leaf extract of Carica papaya Linn. in Sprague Dawley (SD) rats was investigated in this study. The extract was prepared by dissolving the freeze dried extract of the leaves in distilled water and was administered orally to SD rats (consisted of 10 rats/sex/group) at 0 (control), 0.01, 0.14, and 2?g/kg body weight (BW) for 13 weeks. General observation, mortality, and food and water intake were monitored throughout the experimental period. Hematological and biochemical parameters, relative organ weights, and histopathological changes were evaluated. The study showed that leaf extract when administered for 13 weeks did not cause any mortality and abnormalities of behavior or changes in body weight as well as food and water intake. There were no significant differences observed in hematology parameters between treatment and control groups; however significant differences were seen in biochemistry values, for example, LDH, creatinine, total protein, and albumin. However, these changes were not associated with histopathological changes. In conclusion, the results suggested that daily oral administration of rats with C. papaya leaf extract for 13 weeks at a dose up to fourteen times the levels employed in traditional medicine practice did not cause any significant toxic effect. PMID:25530788

  12. Does Carica papaya leaf-extract increase the platelet count? An experimental study in a murine model

    PubMed Central

    Dharmarathna, Sinhalagoda Lekamlage Chandi Asoka; Wickramasinghe, Susiji; Waduge, Roshitha Nilmini; Rajapakse, Rajapakse Peramune Veddikkarage Jayanthe; Kularatne, Senanayake Abeysinghe Mudiyanselage

    2013-01-01

    Objective To investigate the potential role of fresh Carica papaya (C. papaya) leaf extract on haematological and biochemical parameters and toxicological changes in a murine model. Methods In total 36 mice were used for the trial. Fresh C. papaya leaf extract [0.2 mL (2 g)/mouse] was given only to the test group (18 mice). General behavior, clinical signs and feeding patterns were recorded. Blood and tissue samples were collected at intervals. Haematological parameters including platelet, red blood cell (RBC), white blood cell (WBC), packed cell volume (PCV), serum biochemistry including serum creatinine, serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) were determined. Organs for possible histopathological changes were examined. Results Neither group exhibited alteration of behavior or reduction in food and water intake. Similarly, no significant changes in SGOT, SGPT and serum creatinine levels were detected in the test group. Histopathological organ changes were not observed in either group of mice except in three liver samples of the test group which had a mild focal necrosis. The platelet count (11.33±0.35)×105/µL (P=0.000?04) and the RBC count (7.97±0.61)×106/µL (P=0.000?03) were significantly increased in the test group compared to that of the controls. However, WBC count and PCV (%) values were not changed significantly in the test group. The platelet count in the test group started to increase significantly from Day 3 (3.4±0.18×105/µL), reaching almost a fourfold higher at Day 21 (11.3×105/µL), while it was 3.8×105/µL and 5.5×105/µL at Day 3 and Day 21 respectively in the control. Likewise, the RBC count in the test group increased from 6×106/µL to 9×106/ µL at Day 21 while it remained near constant in the control group (6×106/µL). Conclusions Fresh C. papaya leaf extract significantly increased the platelet and RBC counts in the test group as compared to controls. Therefore, it is very important to identify those chemicals of C. papaya leaves as it can be recommended to be used as a medication to boost thrombopoiesis and erythropoiesis in humans and in animals in which these cell lineages have been compromised. PMID:23998013

  13. Metabolomic profiling of the phytomedicinal constituents of Carica papaya L. leaves and seeds by 1H NMR spectroscopy and multivariate statistical analysis.

    PubMed

    Gogna, Navdeep; Hamid, Neda; Dorai, Kavita

    2015-11-10

    Extracts from the Carica papaya L. plant are widely reported to contain metabolites with antibacterial, antioxidant and anticancer activity. This study aims to analyze the metabolic profiles of papaya leaves and seeds in order to gain insights into their phytomedicinal constituents. We performed metabolite fingerprinting using 1D and 2D 1H NMR experiments and used multivariate statistical analysis to identify those plant parts that contain the most concentrations of metabolites of phytomedicinal value. Secondary metabolites such as phenyl propanoids, including flavonoids, were found in greater concentrations in the leaves as compared to the seeds. UPLC-ESI-MS verified the presence of significant metabolites in the papaya extracts suggested by the NMR analysis. Interestingly, the concentration of eleven secondary metabolites namely caffeic, cinnamic, chlorogenic, quinic, coumaric, vanillic, and protocatechuic acids, naringenin, hesperidin, rutin, and kaempferol, were higher in young as compared to old papaya leaves. The results of the NMR analysis were corroborated by estimating the total phenolic and flavonoid content of the extracts. Estimation of antioxidant activity in leaves and seed extracts by DPPH and ABTS in-vitro assays and antioxidant capacity in C2C12 cell line also showed that papaya extracts exhibit high antioxidant activity. PMID:26163870

  14. Genetic differentiation of Colletotrichum gloeosporioides and C. truncatum associated with Anthracnose disease of papaya (Carica papaya L.) and bell pepper (Capsium annuum L.) based on ITS PCR-RFLP fingerprinting.

    PubMed

    Maharaj, Ariana; Rampersad, Sephra N

    2012-03-01

    Members of the genus Colletotrichum include some of the most economically important fungal pathogens in the world. Accurate diagnosis is critical to devising disease management strategies. Two species, Colletotrichum gloeosporioides and C. truncatum, are responsible for anthracnose disease in papaya (Carica papaya L.) and bell pepper (Capsicum annuum L.) in Trinidad. The ITS1-5.8S-ITS2 region of 48 Colletotrichum isolates was sequenced, and the ITS PCR products were analyzed by PCR-RFLP analysis. Restriction site polymorphisms generated from 11 restriction enzymes enabled the identification of specific enzymes that were successful in distinguishing between C. gloeosporioides and C. truncatum isolates. Species-specific restriction fragment length polymorphisms generated by the enzymes AluI, HaeIII, PvuII, RsaI, and Sau3A were used to consistently resolve C. gloeosporioides and C. truncatum isolates from papaya. AluI, ApaI, PvuII, RsaI, and SmaI reliably separated isolates of C. gloeosporioides and C. truncatum from bell pepper. PvuII, RsaI, and Sau3A were also capable of distinguishing among the C. gloeosporioides isolates from papaya based on the different restriction patterns that were obtained as a result of intra-specific variation in restriction enzyme recognition sites in the ITS1-5.8S-ITS2 rDNA region. Of all the isolates tested, C. gloeosporioides from papaya also had the highest number of PCR-RFLP haplotypes. Cluster analysis of sequence and PCR-RFLP data demonstrated that all C. gloeosporioides and C. truncatum isolates clustered separately into species-specific clades regardless of host species. Phylograms also revealed consistent topologies which suggested that the genetic distances for PCR-RFLP-generated data were comparable to that of ITS sequence data. ITS PCR-RFLP fingerprinting is a rapid and reliable method to identify and differentiate between Colletotrichum species. PMID:21720933

  15. Determining sex and screening for the adventitious presence of transgenic material in Carica papaya L. seed germplasm

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya Ringspot Virus (PRSV) is a devastating disease that has a detrimental impact on both commercial papaya production and Caricaceae germplasm conservation. The PRSV coat protein transgenic line 55-1 and derived progeny are resistant to PRSV and have saved the papaya industry in Hawaii. However,...

  16. Antioxidant and Anti-Inflammatory Activities of Extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva Medicinal Plants Collected in Cameroon

    PubMed Central

    Sagnia, Bertrand; Fedeli, Donatella; Casetti, Rita; Montesano, Carla; Falcioni, Giancarlo; Colizzi, Vittorio

    2014-01-01

    Background The vast majority of the population around the world has always used medicinal plants as first source of health care to fight infectious and non infectious diseases. Most of these medicinal plants may have scientific evidence to be considered in general practice. Objective The aim of this work was to investigate the antioxidant capacities and anti-inflammatory activities of ethanol extracts of leaves of Cassia alata, Eleusine indica, Carica papaya, Eremomastax speciosa and the stem bark of Polyscias fulva, collected in Cameroon. Methods Chemiluminescence was used to analyze the antioxidant activities of plant extracts against hydrogen peroxide or superoxide anion. Comet assays were used to analyze the protection against antioxidant-induced DNA damage induced in white blood cells after treating with hydrogen peroxide. Flow cytometry was used to measure ?? T cells proliferation and anti-inflammatory activity of ?? T cells and of immature dendritic cells (imDC) in the presence of different concentrations of plant extracts. Results Ethanol extracts showed strong antioxidant properties against both hydrogen peroxide and superoxide anion. Cassia alata showed the highest antioxidant activity. The effect of plant extracts on ?? T cells and imDC was evidenced by the dose dependent reduction in TNF-? production in the presence of Cassia alata, Carica papaya, Eremomastax speciosa Eleusine indica, and Polyscias fulva. ?? T cells proliferation was affected to the greatest extent by Polyscias fulva. Conclusion These results clearly show the antioxidant capacity and anti-inflammatory activities of plant extracts collected in Cameroon. These properties of leaves and stem bark extracts may contribute to the value for these plants in traditional medicine and in general medical practice. PMID:25090613

  17. Report: In vivo anticoccidial effects of Azadirachta indica and Carica papaya L. with salinomycin drug as a dietary feed supplement in broiler chicks.

    PubMed

    Hema, Srinivasan; Arun, Thangavel; Senthilkumar, Balakrishnan; Senbagam, Duraisamy; Sureshkumar, Muthusamy

    2015-07-01

    A total of thirty suspected broiler chicks were screened for coccidiosis, of them 25 chicks were found to be infected with coccidiosis viz. Eimeria tenella (15) Eimeria maxima (5) Eimeria necatrix (6) and Eimeria mitis (4). The anticoccidial efficacy of Azadirachta indica and Carica papaya with Salinomycin as a dietary feed supplement on the representative E. tenella (25X103 oocyst) infection challenged in broiler chicks was studied in six groups for the period of six weeks. A. indica and C. papaya leaves were administered in powder form at the concentration of 0.1% and 0.2% respectively. The Oocysts per gram (OPG) count were observed on 8th, 9th, 10th, 11th day of post inoculation (DPI). The challenged experimental chicks revealed haemorrhage, thickening of mucosa, cores of blood and ballooning of caecum. The experimental group T5 chicks treated with A. indica were analyzed to possess the maximum weight gain (2.003), better feed conversion ratio (FCR) (2.32), OPG count (5.87), livability percentage (88) and the lesion score (3.33). Chi-square test analysis revealed no significant differences among the treated groups and the performance parameters. Therefore, this study concludes that plant sources used as a remedial curate for coccidiosis is a perforated growth in the commercial broiler industries. PMID:26142532

  18. Bioefficacy of larvicdial and pupicidal properties of Carica papaya (Caricaceae) leaf extract and bacterial insecticide, spinosad, against chikungunya vector, Aedes aegypti (Diptera: Culicidae).

    PubMed

    Kovendan, Kalimuthu; Murugan, Kadarkarai; Naresh Kumar, Arjunan; Vincent, Savariar; Hwang, Jiang-Shiou

    2012-02-01

    The present study was carried out to establish the properties of Carica papaya leaf extract and bacterial insecticide, spinosad on larvicidal and pupicidal activity against the chikungunya vector, Aedes aegypti. The medicinal plants were collected from the area around Bharathiar University, Coimbatore, India. C. papaya leaf was washed with tap water and shade-dried at room temperature. An electrical blender powdered the dried plant materials (leaves). The powder (500 g) of the leaf was extracted with 1.5 l of organic solvents of methanol for 8 h using a Soxhlet apparatus and then filtered. The crude leaf extracts were evaporated to dryness in a rotary vacuum evaporator. The plant extract showed larvicidal and pupicidal effects after 24 h of exposure; however, the highest larval and pupal mortality was found in the leaf extract of methanol C. papaya against the first- to fourth-instar larvae and pupae of values LC(50)?=?I instar was 51.76 ppm, II instar was 61.87 ppm, III instar was 74.07 ppm, and IV instar was 82.18 ppm, and pupae was 440.65 ppm, respectively, and bacterial insecticide, spinosad against the first to fourth instar larvae and pupae of values LC(50)?=?I instar was 51.76 ppm, II instar was 61.87 ppm, III instar was 74.07 ppm, and IV instar was 82.18 ppm, and pupae was 93.44 ppm, respectively. Moreover, combined treatment of values of LC(50)?=?I instar was 55.77 ppm, II instar was 65.77 ppm, III instar was 76.36 ppm, and IV instar was 92.78 ppm, and pupae was 107.62 ppm, respectively. No mortality was observed in the control. The results that the leaves extract of C. papaya and bacterial insecticide, Spinosad is promising as good larvicidal and pupicidal properties of against chikungunya vector, A. aegypti. This is an ideal eco-friendly approach for the control of chikungunya vector, A. aegypti as target species of vector control programs. PMID:21750871

  19. Carica papaya (Cultivated) 6 

    E-print Network

    Monique D. Reed

    2011-08-10

    This project attempts to examine the impact stone fruits can have on the clotting of blood in the human body. Plum rich anthocyanin extract (RAE) was added in different concentrations (1-25ug/ml) to rabbit platelets suspended in Tyrode’s buffer...

  20. Carica papaya Leaves Juice Significantly Accelerates the Rate of Increase in Platelet Count among Patients with Dengue Fever and Dengue Haemorrhagic Fever

    PubMed Central

    Subenthiran, Soobitha; Choon, Tan Chwee; Cheong, Kee Chee; Thayan, Ravindran; Teck, Mok Boon; Muniandy, Prem Kumar; Afzan, Adlin; Abdullah, Noor Rain; Ismail, Zakiah

    2013-01-01

    The study was conducted to investigate the platelet increasing property of Carica papaya leaves juice (CPLJ) in patients with dengue fever (DF). An open labeled randomized controlled trial was carried out on 228 patients with DF and dengue haemorrhagic fever (DHF). Approximately half the patients received the juice, for 3 consecutive days while the others remained as controls and received the standard management. Their full blood count was monitored 8 hours for 48 hours. Gene expression studies were conducted on the ALOX 12 and PTAFR genes. The mean increase in platelet counts were compared in both groups using repeated measure ANCOVA. There was a significant increase in mean platelet count observed in the intervention group (P < 0.001) but not in the control group 40 hours since the first dose of CPLJ. Comparison of mean platelet count between intervention and control group showed that mean platelet count in intervention group was significantly higher than control group after 40 and 48 hours of admission (P < 0.01). The ALOX 12 (FC??=??15.00) and PTAFR (FC??=??13.42) genes were highly expressed among those on the juice. It was concluded that CPLJ does significantly increase the platelet count in patients with DF and DHF. PMID:23662145

  1. Green synthesis and characterization of Carica papaya leaf extract coated silver nanoparticles through X-ray diffraction, electron microscopy and evaluation of bactericidal properties

    PubMed Central

    Banala, Rajkiran Reddy; Nagati, Veera Babu; Karnati, Pratap Reddy

    2015-01-01

    The evolution of nanotechnology and the production of nanomedicine from various sources had proven to be of intense value in the field of biomedicine. The smaller size of nanoparticles is gaining importance in research for the treatment of various diseases. Moreover the production of nanoparticles is eco-friendly and cost effective. In the present study silver nanoparticles were synthesized from Carica papaya leaf extract (CPL) and characterized for their size and shape using scanning electron microscopy and transmission electron microscopy, respectively. Fourier transform infrared spectroscopy (FTIR), Energy dispersive X-ray spectroscopy (EDS/EDX) and X-ray diffraction spectroscopy (XRD) were conducted to determine the concentration of metal ions, the shape of molecules. The bactericidal activity was evaluated using Luria Bertani broth cultures and the minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) were estimated using turbidimetry. The data analysis showed size of 50–250 nm spherical shaped nanoparticles. The turbidimetry analysis showed MIC and MBC was >25 ?g/mL against both Gram positive and Gram negative bacteria in Luria Bertani broth cultures. In summary the synthesized silver nanoparticles from CPL showed acceptable size and shape of nanoparticles and effective bactericidal activity. PMID:26288570

  2. Solenostemon monostachyus, Ipomoea involucrata and Carica papaya seed oil versus Glutathione, or Vernonia amygdalina: Methanolic extracts of novel plants for the management of sickle cell anemia disease

    PubMed Central

    2012-01-01

    Background Sickle cell disease (SCD) is a genetic disease caused by an individual inheriting an allele for sickle cell hemoglobin from both parents and is associated with unusually large numbers of immature blood cells, containing many long, thin, crescent-shaped erythrocytes. It is a disease prevalent throughout many populations. The use of medicinal plants and nutrition in managing SCD is gaining increasing attention. Methods The antisickling effects of Solenostemon monostachyus (SolMon), Carica papaya seed oil (Cari-oil) and Ipomoea involucrata (Ipocrata) in male (HbSSM) and female (HbSSF) human sickle cell blood was examined in vitro and compared with controls, or cells treated with glutathione or an antisickling plant (Vernonia amygdalina; VerMyg). Results Levels of sickle blood cells were significantly reduced (P < 0.05) in all the plant-extract treated SCD patients’ blood compared with that of untreated SCD patients. RBCs in SolMon, Ipocrata, and Cari-oil treated samples were significantly higher (P < 0.05) compared with VerMyg-treated samples. The Fe2+/Fe3+ ratio was significantly reduced (P < 0.05) in all plant extract-treated HbSSM samples compared with controls. Hemoglobin concentration was significantly increased (P < 0.05) by SolMon treatment in HbSSF compared with VerMyg. Sickle cell polymerization inhibition exhibited by SolMon was significantly higher (P < 0.05) compared with that of VerMyg in HbSSF blood. Sickle cell polymerization inhibition in SolMon and Ipocrata were significantly higher (P < 0.05) compared with VerMyg in HbSSM blood. All plant extracts significantly reduced (P < 0.05) lactate dehydrogenase activity in both HbSSM and HbSSF-treated blood. Catalase activity was significantly increased (P < 0.05) in HbSSF blood treated with Ipocrata compared with glutathione. Cari-oil treated HbSSM and HbSSF blood had significantly increased (P < 0.05) peroxidase activity compared with controls. Conclusions Methanolic extracts from S. monostachyus, C. papaya seed oil and I. involucrata exhibited particular antisickling properties coupled with the potential to reduce stress in sickle cell patients. Each plant individually or in combination may be useful for the management of sickle cell disease. PMID:23259718

  3. Characterisation of an acidic peroxidase from papaya (Carica papaya L. cv Tainung No. 2) latex and its application in the determination of micromolar hydrogen peroxide in milk.

    PubMed

    Chen, Li-Chun; Chung, Yun-Chin; Chang, Chen-Tien

    2012-12-15

    An acidic peroxidase isoform, POD-A, with a molecular mass of 69.4 kDa and an isoelectric point of 3.5 was purified from papaya latex. Using o-phenylenediamine (OPD) as a hydrogen donor (citrate-phosphate as pH buffer), the optimum pH for the function of POD-A was 4.6, and the optimum temperature was 50°C. The peroxidase activity of POD-A toward hydrogen donors was both pH- and concentration-dependent. Under optimal conditions, POD-A catalysed the oxidation of OPD at higher rates than pyrogallol, catechol, quercetin and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS). The chemical modification reagents N-bromosuccinimide and sodium azide significantly inhibited POD-A activity. The results of kinetic studies indicated that POD-A followed a ping-pong mechanism and had a K(m) value of 2.8mM for OPD. Using CPC silica-immobilised POD-A for the determination of micromolar H(2)O(2) in milk, the lower limit of determination was 0.1 ?M, and the recoveries of added H(2)O(2) were 96-109%. PMID:22980838

  4. Efficacy of four plant extracts on nematodes associated with papaya in Sindh, Pakistan

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This investigation examines the effect of ethanol extracts of four plant species--Azadirachta indica (neem), Withania somnifera (ashwagandha), Tagetes erecta (marigold) and Eucalyptus citriodora (eucalyptus)--against nematodes associated with papaya (Carica papaya), and it assesses their influence o...

  5. Whole-photosynthesis and transpiration in field-grown papaya plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya (Carica papaya L.) is a principal horticultural crop of tropical and subtropical regions. Knowledge of papaya response to environmental factors provides a scientific basis to develop management strategies to optimize fruit yield and quality. In papaya, the photosynthetic capacity also influ...

  6. The origin of the non-recombining region of sex chromosomes in Carica and Vasconcellea

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Carica and Vasconcellea are two closely related sister genera in the family Caricaceae, and were once classified as two sections under Carica. After the section Vasconcellea with 21 species was reinstated as a separate genus based on molecular marker data, papaya became the sole species in Carica. S...

  7. Barriers in a tropical orchard landscape: how the presence or absence of hedges influences insect dispersal in mixed orchards of Carica papaya and Manilkara zapota.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The presence of plants in the form of a hedge may hinder or alter movement of certain insects into and within an orchard. We investigated the impact that the presence of a hedge of tall grass had on infestation by various pests into mixed orchards of papaya and sapodilla. For most insects there was ...

  8. A 1H NMR Investigation of the Interaction between Phenolic Acids Found in Mango (Manguifera indica cv Ataulfo) and Papaya (Carica papaya cv Maradol) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) Free Radicals

    PubMed Central

    López-Martínez, Luis M.; Santacruz-Ortega, Hisila; Navarro, Rosa-Elena; Sotelo-Mundo, Rogerio R.; González-Aguilar, Gustavo A.

    2015-01-01

    The benefits of phenolic acids on human health are very often ascribed to their potential to counteract free radicals to provide antioxidant protection. This potential has been attributed to their acidic chemical structure, which possesses hydroxyl groups in different positions. Phenolic acids can interact between themselves and exhibit an additive, antagonistic or synergistic effect. In this paper, we used 1H NMR to analyze the interactions and mechanisms that are present in major phenolic acids found in mango (gallic, protocatechuic, chlorogenic and vanillic acids) and papaya (caffeic, ferulic and p-coumaric acids), and the DPPH radical was used to evaluate the effect of the antioxidant mixtures. The interactions were found to occur via hydrogen bonds between the -OH and -COOH groups. Moreover, the phenolic acids exhibit two types of mechanisms for the neutralization of the DPPH radical. According to the results, these two mechanisms are Hydrogen Atom Transfer (HAT) and Single Electron Transfer (SET). The ability of the phenolic acid to neutralize the DPPH radical decreases in the following order in mango: gallic > chlorogenic > protocatechuic > vanillic. Moreover, within the acids found in papaya, the order was as follows: caffeic > p-coumaric > ferulic. PMID:26559189

  9. 76 FR 13972 - Notice of Availability of a Pest Risk Analysis for the Importation of Fresh Papaya Fruit From...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-03-15

    ... Importation of Fresh Papaya Fruit From Malaysia Into the Continental United States AGENCY: Animal and Plant... of fresh papaya (Carica papaya) fruit from Malaysia into the continental United States. Based on our... commercial consignment. APHIS received a request from the Government of Malaysia to allow the importation...

  10. Managing oriental fruit fly (Diptera: Tephritidae), with spinosad-based protein bait sprays and sanitation in papaya orchards in Hawaii.

    PubMed

    Piñero, Jaime C; Mau, Ronald F L; Vargas, Roger I

    2009-06-01

    The efficacy of GF-120 NF Naturalyte Fruit Fly Bait in combination with field sanitation was assessed as a control for female oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in papaya (Carica papaya L.) orchards in Hawaii. Three different bait spray regimes were evaluated: every row (high use of the bait), every fifth row (moderate use), and every 10th row (low use). Orchard plots in which no bait was applied served as controls. For five of the seven biweekly periods that followed the first bait spray, trapping data revealed significantly fewer female B. dorsalis captured in plots subject to high and moderate bait use than in control plots. Differences in incidence of infestation among treatments were detected only by the third (12 wk after first spray) fruit sampling with significantly fewer infested one-fourth to one-half ripe papaya fruit in plots subject to high and moderate bait use than in control plots. Parasitism rates by Fopius arisanus (Sonan) (Hymenoptera: Braconidae) were not negatively affected by bait application. Results indicate that foliar applications of GF-120 NF Naturalyte Fruit Fly Bait either to all rows (every other tree), or to every fifth row (every tree) in combination with good sanitation can effectively reduce infestation by B. dorsalis in papaya orchards in Hawaii. PMID:19610428

  11. Analysis of genetic diversity of southern Spain fig tree (Ficus carica L.) and reference materials as a tool for breeding and conservation.

    PubMed

    Perez-Jiménez, M; López, B; Dorado, G; Pujadas-Salvá, A; Guzmán, G; Hernandez, P

    2012-06-01

    The common fig tree (Ficus carica L.) is a Mediterranean crop with problematic cultivar identification. The recovery and conservation of possible local varieties for ecological production requires the previous genetic characterization of the available germplasm. In this context, 42 lines corresponding to 12 local varieties and two caprifigs, in addition to 15 reference samples have been fingerprinted using 21 SSR markers. A total of 77 alleles were revealed, detecting a useful level of genetic variability within the local germplasm pools. UPGMA clustering analysis has revealed the genetic structure and relationships among the local and reference germplasm. Eleven of the local varieties could be identified and defined as obtained clusters, showing that SSR analysis is an efficient method to evaluate the Andalusian fig tree diversity for on-farm conservation. PMID:22804343

  12. 110 Plant Protection Quarterly Vol.19(3) 2004 The papaya mealybug (PM), Paracoc-

    E-print Network

    Reddy, Gadi VP

    2002 and was causing serious damage to papaya, Plumeria spp., Hibiscus spp. and other plants such as Annona squamosa, Carica papaya, Hibiscus rosa-sinensis, Ipomoea spp., Manihot esculenta and Solanum in Plumeria spp. (Apocynaceae) plants. On Hibiscus spp. (Malvaceae) the tender shoots covered by mealybugs

  13. Release and establishment of Encarsia diaspidicola (Hymenoptera:Aphelididae) against white peach scale in papaya

    Technology Transfer Automated Retrieval System (TEKTRAN)

    White peach scale, Pseudaulacaspis pentagona (Hemiptera:Diaspididae) is a serious economic pest of papaya, Carica papaya L. The parasitic wasp Encarsia diaspidicola (Hymenoptera: Aphelinidae) was brought from Samoa into a quarantine containment facility in Hawaii for evaluation and potential release...

  14. METHYL JASMONATE REDUCES DECAY AND MAINTAINS POSTHARVEST QUALITY OF PAPAYA 'SUNRISE'

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Exposure of papaya (Carica papaya L., cv. Sunrise) fruit to methyl jasmonate (MJ) vapors (ten to the -5 or ten to the -4 M) for 16 h at 20 degrees C inhibited fungal decay and reduced chilling injury development and loss of firmness during storage for 14-32 days at 10 degrees C and shelf life (4 day...

  15. Monoclonal antibodies to the two most basic papaya proteinases.

    PubMed

    Goodenough, P W; Kilshaw, P J; McEwan, F; Owen, A J

    1986-08-01

    The proteinases from Carica papaya include papain, isoenzymes of chymopapain and two proteinases A and B distinguished by their unusually high pI. The identity of one of the most basic proteinases has been questioned. The present report describes the preparation and characterisation of two monoclonal antibodies that react specifically with papaya proteinases A and B respectively and a third that identifies a common structural feature found in papain and proteinase A. PMID:3545314

  16. Gene Technology for Papaya Ringspot Virus Disease Management

    PubMed Central

    Azad, Md. Abul Kalam; Sidik, Nik Marzuki

    2014-01-01

    Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase gene-mediated transformation for effective PRSV disease management. The development of PRSV-resistant papaya via post-transcriptional gene silencing is a promising technology for PRSV disease management. PRSV-resistant transgenic papaya is environmentally safe and has no harmful effects on human health. Recent studies have revealed that the success of adoption of transgenic papaya depends upon the application, it being a commercially viable product, bio-safety regulatory issues, trade regulations, and the wider social acceptance of the technology. This review discusses the genome and the genetic diversity of PRSV, host range determinants, molecular diagnosis, disease management strategies, the development of transgenic papaya, environmental issues, issues in the adoption of transgenic papaya, and future directions for research. PMID:24757435

  17. Gene technology for papaya ringspot virus disease management.

    PubMed

    Azad, Md Abul Kalam; Amin, Latifah; Sidik, Nik Marzuki

    2014-01-01

    Papaya (Carica papaya) is severely damaged by the papaya ringspot virus (PRSV). This review focuses on the development of PRSV resistant transgenic papaya through gene technology. The genetic diversity of PRSV depends upon geographical distribution and the influence of PRSV disease management on a sequence of PRSV isolates. The concept of pathogen-derived resistance has been employed for the development of transgenic papaya, using a coat protein-mediated, RNA-silencing mechanism and replicase gene-mediated transformation for effective PRSV disease management. The development of PRSV-resistant papaya via post-transcriptional gene silencing is a promising technology for PRSV disease management. PRSV-resistant transgenic papaya is environmentally safe and has no harmful effects on human health. Recent studies have revealed that the success of adoption of transgenic papaya depends upon the application, it being a commercially viable product, bio-safety regulatory issues, trade regulations, and the wider social acceptance of the technology. This review discusses the genome and the genetic diversity of PRSV, host range determinants, molecular diagnosis, disease management strategies, the development of transgenic papaya, environmental issues, issues in the adoption of transgenic papaya, and future directions for research. PMID:24757435

  18. Genetic diversity of papaya ring spot virus in Puerto Rico

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Food and Agriculture Organization estimates that 20-40% of crop yield is lost due to pests and diseases. Viruses are agents that cause diseases which contribute greatly to the global yield loss. Because of this, food production is negatively affected, especially in the tropics. Carica papaya, co...

  19. Development of Transgenic Papaya through Agrobacterium-Mediated Transformation

    PubMed Central

    Azad, Md. Abul Kalam; Rabbani, Md. Golam; Amin, Latifah; Sidik, Nik Marzuki

    2013-01-01

    Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and ?-glucuronidase (GUS) as the reporter gene. The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium containing 500?mg/L carbenicillin?+?200?mg/L cefotaxime for one week. The cocultivated explants were transferred into the final selection medium containing 500?mg/L carbenicillin?+?200?mg/L cefotaxime?+?50?mg/L kanamycin for callus induction as well as plant regeneration. The callus derived from the hypocotyls of Carica papaya cv. Shahi showed the highest positive GUS activities compared to Carica papaya cv. Ranchi. The transformed callus grew vigorously and formed embryos followed by transgenic plantlets successfully. The result of this study showed that the hypocotyls of C. papaya cv. Shahi and C. papaya cv. Ranchi are better explants for genetic transformation compared to immature embryos. The transformed C. papaya cv. Shahi also showed the maximum number of plant regeneration compared to that of C. papaya cv. Ranchi. PMID:24066284

  20. Effects of chemical treatments on fresh-cut papaya.

    PubMed

    Albertini, Silvana; Lai Reyes, Andrés Enrique; Trigo, Juliana Moreno; Sarriés, Gabriel Adrián; Spoto, Marta Helena Fillet

    2016-01-01

    Four treatments (control, 0.1% cinnamaldehyde, 0.75% calcium chloride and combination of 0.1% cinnamaldehyde and 0.75% calcium chloride) were used to evaluate chemical effects on shelf life, quality and sensory acceptability of fresh-cut papaya (Carica papaya L.). Papaya slices were packed and covered with polypropylene film, stored at 5 °C; and evaluated after 1, 3, 6, 9, 12, and 15 days for microbiological and physicochemical changes. A sensory evaluation was performed at 1, 3, 6, 9, and 12 days. There was no occurrence of Salmonella, Escherichia coli or psychotropic bacteria. The cinnamaldehyde alone and a combination of cinnamaldehyde and calcium chloride treatments yielded better control of the total coliforms. The combination treatment decreased the CO2 concentration and increased the maintenance of papaya firmness. All the treatments had acceptability. The combination treatment was the most effective treatment for flavor, taste, and preservation until day 12. PMID:26213093

  1. Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray

    PubMed Central

    2012-01-01

    Background Papaya (Carica papaya L.) is a commercially important crop that produces climacteric fruits with a soft and sweet pulp that contain a wide range of health promoting phytochemicals. Despite its importance, little is known about transcriptional modifications during papaya fruit ripening and their control. In this study we report the analysis of ripe papaya transcriptome by using a cross-species (XSpecies) microarray technique based on the phylogenetic proximity between papaya and Arabidopsis thaliana. Results Papaya transcriptome analyses resulted in the identification of 414 ripening-related genes with some having their expression validated by qPCR. The transcription profile was compared with that from ripening tomato and grape. There were many similarities between papaya and tomato especially with respect to the expression of genes encoding proteins involved in primary metabolism, regulation of transcription, biotic and abiotic stress and cell wall metabolism. XSpecies microarray data indicated that transcription factors (TFs) of the MADS-box, NAC and AP2/ERF gene families were involved in the control of papaya ripening and revealed that cell wall-related gene expression in papaya had similarities to the expression profiles seen in Arabidopsis during hypocotyl development. Conclusion The cross-species array experiment identified a ripening-related set of genes in papaya allowing the comparison of transcription control between papaya and other fruit bearing taxa during the ripening process. PMID:23256600

  2. Papaya Ringspot Virus

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The term papaya ringspot virus (PRSV) was coined by Jensen in 1949, to describe a papaya disease in Hawaii. Later work showed that diseases such as papaya mosaic and watermelon mosaic virus-1 were caused by PRSV. The primary host range of PRSV is papaya and cucurbits, with Chenopium amaranticolor ...

  3. Analysis of Papaya Cell Wall-Related Genes during Fruit Ripening Indicates a Central Role of Polygalacturonases during Pulp Softening

    PubMed Central

    Fabi, João Paulo; Broetto, Sabrina Garcia; da Silva, Sarah Lígia Garcia Leme; Zhong, Silin; Lajolo, Franco Maria; do Nascimento, João Roberto Oliveira

    2014-01-01

    Papaya (Carica papaya L.) is a climacteric fleshy fruit that undergoes dramatic changes during ripening, most noticeably a severe pulp softening. However, little is known regarding the genetics of the cell wall metabolism in papayas. The present work describes the identification and characterization of genes related to pulp softening. We used gene expression profiling to analyze the correlations and co-expression networks of cell wall-related genes, and the results suggest that papaya pulp softening is accomplished by the interactions of multiple glycoside hydrolases. The polygalacturonase cpPG1 appeared to play a central role in the network and was further studied. The transient expression of cpPG1 in papaya results in pulp softening and leaf necrosis in the absence of ethylene action and confirms its role in papaya fruit ripening. PMID:25162506

  4. Thrombocyte counts in mice after the administration of papaya leaf suspension.

    PubMed

    Sathasivam, Kathiresan; Ramanathan, Surash; Mansor, Sharif M; Haris, Mas Rosemal M H; Wernsdorfer, Walther H

    2009-10-01

    Following up a popular use of crude leaf preparations from Carica papaya for the treatment of dengue infections, a suspension of powdered Carica papaya leaves in palm oil has been investigated for its effect on thrombocyte counts in mice, administering by gavage 15 mg of powdered leaves per kg body weight to 5 mice. Equal numbers of animals received corresponding volumes of either palm oil alone or physiological saline solution. Thrombocyte counts before and at 1, 2, 4, 8, 10, 12, 24, 48 and 72 hours after dosing revealed significantly higher mean counts at 1, 2, 4, 8, 10 and 12 after dosing with the C. papaya leaf formulation as compared to the mean count at hour 0. There was only a non-significant rise of thrombocyte counts in the group having received saline solution, possibly the expression of a normal circadian rhythm in mice. The group having received palm oil only showed a protracted increase of platelet counts that was significant at hours 8 and 48 and obviously the result of a hitherto unknown stimulation of thrombocyte release. The results call for a dose-response investigation and for extending the studies to the isolation and identification of the C. papaya substances responsible for the release and/or production of thrombocytes. PMID:19915811

  5. Plant Regeneration and Somatic Embryogenesis from Immature Embryos Derived through Interspecific Hybridization among Different Carica Species

    PubMed Central

    Azad, Md. Abul Kalam; Rabbani, Md. Golam; Amin, Latifah

    2012-01-01

    Plant regeneration and somatic embryogenesis through interspecific hybridization among different Carica species were studied for the development of a papaya ringspot virus-resistant variety. The maximum fruit sets were recorded from the cross of the native variety C. papaya cv. Shahi with the wild species C. cauliflora. The highest hybrid embryos were recorded at 90 days after pollination and the embryos were aborted at 150 days after pollination. The immature hybrid embryos were used for plant regeneration and somatic embryogenesis. The 90-day-old hybrid embryos from the cross of C. papaya cv. Shahi × C. cauliflora showed the highest percentage of germination, as well as plant regeneration on growth regulators free culture medium after 7 days pre-incubation on half-strength MS medium supplemented with 0.2 mg/L BAP, 0.5 mg/L NAA and 60 g/L sucrose. The 90-day-old hybrid embryos from the cross of C. papaya cv. Shahi × C. cauliflora produced maximum callus, as well as somatic embryos when cultured on half-strength MS medium containing 5 mg/L 2,4-D, 100 mg/L glutamine, 100 mg/L casein hydrolysate and 60 g/L sucrose. The somatic embryos were transferred into half-strength MS medium containing 0.5 mg/L BAP and 0.2 mg/L NAA and 60 g/L sucrose for maturation. The highest number of regenerated plants per hybrid embryo (10.33) was recorded from the cross of C. papaya cv. Shahi × C. cauliflora. Isoenzyme and dendrogram cluster analysis using UPGMA of the regenerated F1 plantlets confirmed the presence of the hybrid plantlets. PMID:23235330

  6. Beverages of lemon juice and exotic noni and papaya with potential for anticholinergic effects.

    PubMed

    Gironés-Vilaplana, Amadeo; Valentão, Patrícia; Andrade, Paula B; Ferreres, Federico; Moreno, Diego A; García-Viguera, Cristina

    2015-03-01

    Lemon (Citrus limon (L.) Burm. f.) juice beverages enriched either with noni (Morinda citrifolia L.) (LN) or papaya (Carica papaya L.) (LP), were characterized by HPLC-DAD-ESI/MS(n), the antioxidant capacity was evaluated by (DPPH·), superoxide (O2(·-)), hydroxyl radicals (·OH) and hypochlorous acid (HOCl) assays, and their potential as acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitors was also assessed. The fruits are rich in a wide range of bioactive phenolics. Regarding DPPH·, ·OH and HOCl assays, the LP displayed strong activity, and LN was the most active against O2(·-). Concerning cholinesterases, LP was the most active, mainly due to lemon juice contribution. The effect on the cholinesterases was not as strong as in previous reports on purified extracts, but the bioactive-rich beverages offer the possibility of dietary coadjutants for daily consumption of health-promoting substances by adults with aging-related cognitive or physical disorders. PMID:25306312

  7. tRNALeu intron (UAA) of Ficus carica L.: genetic diversity and evolutionary patterns.

    PubMed

    Baraket, G; Abdelkrim, A B; Salhi-Hannachi, A

    2015-01-01

    Cytoplasmic chloroplast DNA was explored to establish genetic relationships among Ficus carica cultivars and elucidate the molecular evolution of the species. The results suggest the occurrence of haplotype and nucleotide diversity. Conserved group I intron sequence motifs were detected and showed a common secondary structure, despite the presence of some mutations on their sequences. The neighbor-joining dendrogram showed a continuous diversity that characterizes local resources. The maximum parsimony tree, with an RI index of 0.507, indicated minimal homoplasy within the data set. Furthermore, our results demonstrate that the trnL intron is the seat of numerous substitutions. Herein, new insight on the mechanism involved in the evolution of the trnL intron in the fig is presented. From the study, it appears that there is an explicit rejection of the null hypothesis in F. carica. A scenario of positive selection and recent expansion of F. carica genotypes across Tunisia seems to be retained. PMID:25966152

  8. High-density linkage mapping revealed suppression of recombination at the sex determination locus in papaya.

    PubMed Central

    Ma, Hao; Moore, Paul H; Liu, Zhiyong; Kim, Minna S; Yu, Qingyi; Fitch, Maureen M M; Sekioka, Terry; Paterson, Andrew H; Ming, Ray

    2004-01-01

    A high-density genetic map of papaya (Carica papaya L.) was constructed using 54 F(2) plants derived from cultivars Kapoho and SunUp with 1501 markers, including 1498 amplified fragment length polymorphism (AFLP) markers, the papaya ringspot virus coat protein marker, morphological sex type, and fruit flesh color. These markers were mapped into 12 linkage groups at a LOD score of 5.0 and recombination frequency of 0.25. The 12 major linkage groups covered a total length of 3294.2 cM, with an average distance of 2.2 cM between adjacent markers. This map revealed severe suppression of recombination around the sex determination locus with a total of 225 markers cosegregating with sex types. The cytosine bases were highly methylated in this region on the basis of the distribution of methylation-sensitive and -insensitive markers. This high-density genetic map is essential for cloning of specific genes of interest such as the sex determination gene and for the integration of genetic and physical maps of papaya. PMID:15020433

  9. The Effect of Fermented Papaya Preparation on Radioactive Exposure.

    PubMed

    Fibach, Eitan; Rachmilewitz, Eliezer A

    2015-09-01

    Exposure to ionizing radiation causes cellular damage, which can lead to premature cell death or accumulation of somatic mutations, resulting in malignancy. The damage is mediated in part by free radicals, particularly reactive oxygen species. Fermented papaya preparation (FPP), a product of yeast fermentation of Carica papaya Linn, has been shown to act as an antioxidant. In this study, we investigated the potential of FPP to prevent radiation-induced damage. FPP (0-100 ?g/ml) was added to cultured human foreskin fibroblasts and myeloid leukemia (HL-60) cells either before or after irradiation (0-18 Gy). After 1-3 days, the cells were assayed for: intracellular labile iron, measured by staining with calcein; reactive oxygen species generation, measured with dichlorofluorescein diacetate; apoptosis, determined by phosphatidylserine exposure; membrane damage, determined by propidium iodide uptake; and cell survival, determined by a cell proliferation assay. DNA damage was estimated by measuring 8-oxoguanine, a parameter of DNA oxidation, using a fluorescent-specific probe and by the comet assay. These parameters were also assayed in bone marrow cells of mice treated with FPP (by adding it to the drinking water) either before or after irradiation. Somatic mutation accumulation was determined in their peripheral red blood cells, and their survival was monitored. FPP significantly reduced the measured radiation-induced cytotoxic parameters. These findings suggest that FPP might serve as a radioprotector, and its effect on DNA damage and mutagenicity might reduce the long-term effects of radiation, such as primary and secondary malignancy. PMID:26291738

  10. Papaya Extract to Treat Dengue: A Novel Therapeutic Option?

    PubMed Central

    Sarala, N; Paknikar, SS

    2014-01-01

    Dengue is a viral disease that today affects a vast number of people in over 125 countries and is responsible for a sizable number of deaths. In the absence of an effective antiviral drug to treat the disease, various treatments are being investigated. Studies have indicated that the juice of the leaves of the Carica papaya plant from the family Caricaceae could help to increase the platelet levels in these patients. This review describes some of the published studies on this topic. The search was done independently by the two authors using PubMed, Google and the library database and included relevant articles of the last 10 years. A total of 7 studies were included in this review, which were one animal study, one case report, three case series and two randomized controlled trials. Although many of the studies and case reports published in literature lack adequate information, some of the studies do raise the possibility that this treatment could be an important option in the future. Further large-scale studies could establish the usefulness or ineffectiveness of this natural product in the treatment of dengue. PMID:24971201

  11. Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China

    PubMed Central

    Zhao, Guangyuan; Shen, Wentao; Tuo, Decai; Li, Xiaoying

    2015-01-01

    The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya. PMID:26358610

  12. Managing papaya ringspot virus: Impact of grass barriers on alate aphid immigration into papaya orchards

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya ringspot virus, transmitted by alate aphids, is the most limiting factor of papaya production in the Caribbean region. Although there are transgenic papaya varieties that provide protection from this virus, these varieties are effective only in certain regions against certain strains of the v...

  13. Is the old world fig, Ficus carica L. (Moraceae), an alternate host for the Asian citrus psyllid, Diaphorina citri (Kuwayama) (Homoptera: Psyllidae)?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The only non-rutaceous plant on which D. citri has been found breeding in Texas is the edible fig, Ficus carica (Moraceae). In the summer of 2010, we discovered D. citri nymphs on a dooryard fig tree. Fig has its own species of psyllid, Homatoma ficus, but both adults and nymphs of that species ar...

  14. COMMERCIALIZATION OF TRANSGENIC PAPAYA: WEIGHING BENEFITS AND POTENTIAL RISKS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya is Hawaii's second most important fruit crop, behind the pineapple. In 1992, papaya ringspot virus (PRSV) was discovered in Puna Hawaii, where 95% of Hawaii's papaya were being grown. By late 1994, PRSV was widespread in Puna and the papaya industry was facing severe crop loss. In 1998, tr...

  15. Cancer chemopreventive effects of the flavonoid-rich fraction isolated from papaya seeds.

    PubMed

    Pathak, Neelam; Khan, Saba; Bhargava, Arpit; Raghuram, Gorantla V; Jain, Deepika; Panwar, Hariom; Samarth, Ravindra M; Jain, Subodh K; Maudar, Kewal K; Mishra, Dinesh K; Mishra, Pradyumna K

    2014-01-01

    Intervention to decelerate, arrest, or reverse the process of carcinogenesis by the use of either natural or synthetic agents individually or in combination has emerged as a promising and pragmatic medical approach to reduce cancer risk. In the present study, we examined the cancer chemopreventive potential of a flavonoid-rich fraction isolated from the seeds of Carica papaya, a plant traditionally referred to as papaw. The flavonoid-enriched benzene fraction of the aqueous extract exerted its anticancer properties in vitro through cytoprotection, antioxidative and antiinflammatory mechanisms and genoprotection in response to isocyanate-induced carcinogenicity. Medium-term anticarcinogenicity and 2-stage skin papillomagenesis studies conducted in benzopyrene-induced lung carcinogenesis and 7,12-dimethyl benz(a)anthracene-mediated skin papillomagenesis mouse models further validated our in vitro observations. This is the first demonstration of chemopreventive activities of papaya seed products, however, further studies to understand the subtle targets of intracellular signaling pathways, pharmacological profile and toxicological safety of this bioactive fraction are essential to pave the way for successful clinical translation. Our study supports the inverse association between dietary flavonoid intake and cancer risk. PMID:24820939

  16. Informativeness of minisatellite and microsatellite markers for genetic analysis in papaya.

    PubMed

    Oliveira, G A F; Dantas, J L L; Oliveira, E J

    2015-10-01

    The objective of this study was to evaluate information on minisatellite and microsatellite markers in papaya (Carica papaya L.). Forty minisatellites and 91 microsatellites were used for genotyping 24 papaya accessions. Estimates of genetic diversity, genetic linkage and analyses of population structure were compared. A lower average number of alleles per locus was observed in minisatellites (3.10) compared with microsatellites (3.57), although the minisatellites showed rarer alleles (18.54 %) compared with microsatellite (13.85 %). Greater expected (He = 0.52) and observed (Ho = 0.16) heterozygosity was observed in the microsatellites compared with minisatellites (He = 0.42 and Ho = 0.11), possibly due to the high number of hermaphroditic accessions, resulting in high rates of self-fertilization. The polymorphic information content and Shannon-Wiener diversity were also higher for microsatellites (from 0.47 to 1.10, respectively) compared with minisatellite (0.38 and 0.85, respectively). The probability of paternity exclusion was high for both markers (>0.999), and the combined probability of identity was from 1.65(-13) to 4.33(-38) for mini- and micro-satellites, respectively, which indicates that both types of markers are ideal for genetic analysis. The Bayesian analysis indicated the formation of two groups (K = 2) for both markers, although the minisatellites indicated a substructure (K = 4). A greater number of accessions with a low probability of assignment to specific groups were observed for microsatellites. Collectively, the results indicated higher informativeness of microsatellites. However, the lower informative power of minisatellites may be offset by the use of larger number of loci. Furthermore, minisatellites are subject to less error in genotyping because there is greater power to detect genotyping systems when larger motifs are used. PMID:26280323

  17. Genotoxic and Cytotoxic Safety Evaluation of Papain (Carica papaya L.) Using In Vitro Assays

    PubMed Central

    da Silva, Claudia R.; Oliveira, Marcia B. N.; Motta, Ellen S.; de Almeida, Gabriella S.; Varanda, Leandro L.; de Pádula, Marcelo; Leitão, Alvaro C.; Caldeira-de-Araújo, Adriano

    2010-01-01

    Papain, a phytotherapeutic agent, has been used in the treatment of eschars and as a debriding chemical agent to remove damaged or necrotic tissue of pressure ulcers and gangrene. Its benefits in these treatments are deemed effective, since more than 5000 patients, at the public university hospital at Rio de Janeiro, Brazil, have undergone papain treatment and presented satisfactory results. Despite its extensive use, there is little information about toxic and mutagenic properties of papain. This work evaluated the toxic and mutagenic potential of papain and its potential antioxidant activity against induced-H2O2 oxidative stress in Escherichia coli strains. Cytotoxicity assay, Growth inhibition test, WP2-Mutoxitest and Plasmid-DNA treatment, and agarose gel electrophoresis were used to investigate if papain would present any toxic or mutagenic potential as well as if papain would display antioxidant properties. Papain exhibited negative results for all tests. This agent presented an activity protecting cells against H2O2-induced mutagenesis. PMID:20508844

  18. Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning

    PubMed Central

    Tuo, Decai; Shen, Wentao; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2015-01-01

    Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion® Cloning Kit (Clontech, Mountain View, CA, USA), was used to construct intron-less or intron-containing full-length cDNA clones of the isolate PLDMV-DF, with the simultaneous scarless assembly of multiple viral and intron fragments into a plasmid vector in a single reaction. The intron-containing full-length cDNA clone of PLDMV-DF was stably propagated in Escherichia coli. In vitro intron-containing transcripts were processed and spliced into biologically active intron-less transcripts following mechanical inoculation and then initiated systemic infections in Carica papaya L. seedlings, which developed similar symptoms to those caused by the wild-type virus. However, no infectivity was detected when the plants were inoculated with RNA transcripts from the intron-less construct because the instability of the viral cDNA clone in bacterial cells caused a non-sense or deletion mutation of the genomic sequence of PLDMV-DF. To our knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation. In-Fusion cloning shortens the construction time from months to days. Therefore, it is a faster, more flexible, and more efficient method than the traditional multistep restriction enzyme-mediated subcloning procedure. PMID:26633465

  19. Rapid Construction of Stable Infectious Full-Length cDNA Clone of Papaya Leaf Distortion Mosaic Virus Using In-Fusion Cloning.

    PubMed

    Tuo, Decai; Shen, Wentao; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2015-01-01

    Papaya leaf distortion mosaic virus (PLDMV) is becoming a threat to papaya and transgenic papaya resistant to the related pathogen, papaya ringspot virus (PRSV). The generation of infectious viral clones is an essential step for reverse-genetics studies of viral gene function and cross-protection. In this study, a sequence- and ligation-independent cloning system, the In-Fusion(®) Cloning Kit (Clontech, Mountain View, CA, USA), was used to construct intron-less or intron-containing full-length cDNA clones of the isolate PLDMV-DF, with the simultaneous scarless assembly of multiple viral and intron fragments into a plasmid vector in a single reaction. The intron-containing full-length cDNA clone of PLDMV-DF was stably propagated in Escherichia coli. In vitro intron-containing transcripts were processed and spliced into biologically active intron-less transcripts following mechanical inoculation and then initiated systemic infections in Carica papaya L. seedlings, which developed similar symptoms to those caused by the wild-type virus. However, no infectivity was detected when the plants were inoculated with RNA transcripts from the intron-less construct because the instability of the viral cDNA clone in bacterial cells caused a non-sense or deletion mutation of the genomic sequence of PLDMV-DF. To our knowledge, this is the first report of the construction of an infectious full-length cDNA clone of PLDMV and the splicing of intron-containing transcripts following mechanical inoculation. In-Fusion cloning shortens the construction time from months to days. Therefore, it is a faster, more flexible, and more efficient method than the traditional multistep restriction enzyme-mediated subcloning procedure. PMID:26633465

  20. Ancient and modern occurrences of common fig (Ficus carica L.) in the British isles

    NASA Astrophysics Data System (ADS)

    Dickson, James H.; Dickson, Camilla

    Knowledge of the reproductive biology of the common fig ( Ficus carica) is essential for the interpretation of present and past occurrences of pips from archaeological layers as well as for understanding the status of trees, cultivated or wild. Only parthenocarpic varieties ripen figs in Britain and these cannot produce fertile pips. Common figs growing wild in Britain all come from pips from imported figs, often figs that had been eaten and the pips evacuated. There are many discoveries of pips from Roman and later urban and military sites in Britain. These pips too were derived from imported figs and not from locally cultivated trees. There is no proof that the Romans grew common fig in Britain and the earliest documentary evidence of cultivation is as late as the 15th century A.D.

  1. Transgenic Papaya: Can We Proceed Beyond the Hawaiian Experience?

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The story of the development, deregulation, and commercialization of the papaya ringspot virus (PRSV) resistant transgenic SunUp and Rainbow papaya for Hawaii is quite well known at least among plant virologist and knowledgeable people in the field of papaya. Thus, the story will be only briefly r...

  2. Transgenic Papaya: Development, Release, Impact, and Challenges

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Although the technology for developing virus-resistant transgenic plants through the use of the coat protein of a virus was unveiled twenty years ago, it is surprising to note that only a three virus-resistant plants (squash, potato, and papaya) have been commercialized in the U.S. The transgenic p...

  3. The Relationship between the Expression of Ethylene-Related Genes and Papaya Fruit Ripening Disorder Caused by Chilling Injury

    PubMed Central

    Zou, Yuan; Zhang, Lin; Rao, Shen; Zhu, Xiaoyang; Ye, Lanlan; Chen, Weixin; Li, Xueping

    2014-01-01

    Papaya (Carica papaya L.) is sensitive to low temperature and easy to be subjected to chilling injury, which causes fruit ripening disorder. This study aimed to investigate the relationship between the expression of genes related to ethylene and fruit ripening disorder caused by chilling injury. Papaya fruits were firstly stored at 7°C and 12°C for 25 and 30 days, respectively, then treated with exogenous ethylene and followed by ripening at 25°C for 5 days. Chilling injury symptoms such as pulp water soaking were observed in fruit stored at 7°C on 20 days, whereas the coloration and softening were completely blocked after 25 days, Large differences in the changes in the expression levels of twenty two genes involved in ethylene were seen during 7°C-storage with chilling injury. Those genes with altered expression could be divided into three groups: the group of genes that were up-regulated, including ACS1/2/3, EIN2, EIN3s/EIL1, CTR1/2/3, and ERF1/3/4; the group of genes that were down-regulated, including ACO3, ETR1, CTR4, EBF2, and ERF2; and the group of genes that were un-regulated, including ACO1/2, ERS, and EBF1. The results also showed that pulp firmness had a significantly positive correlation with the expression of ACS2, ACO1, CTR1/4, EIN3a/b, and EBF1/2 in fruit without chilling injury. This positive correlation was changed to negative one in fruit after storage at 7°C for 25 days with chilling injury. The coloring index displayed significantly negative correlations with the expression levels of ACS2, ACO1/2, CTR4, EIN3a/b, ERF3 in fruit without chilling injury, but these correlations were changed into the positive ones in fruit after storage at 7°C for 25 days with chilling injury. All together, these results indicate that these genes may play important roles in the abnormal softening and coloration with chilling injury in papaya. PMID:25542021

  4. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-28

    ...importation of commercial shipments of fresh papayas from Colombia and Ecuador...requirements for field sanitation, hot water treatment, and fruit fly trapping...of commercial consignments of fresh papayas from Colombia and Ecuador...field sanitation, and hot water treatment be employed to...

  5. A ripening associated peroxidase from papaya having a role in defense and lignification: heterologous expression and in-silico and in-vitro experimental validation.

    PubMed

    Pandey, Veda P; Dwivedi, Upendra N

    2015-01-25

    Fruit ripening associated full length cDNA of a peroxidase from papaya was cloned and heterologously expressed. The expressed peroxidase was activated by in-vitro re-folding in the presence of hemin and calcium. The purified recombinant peroxidase exhibited broad substrate affinity in the order of o-dianisidine>pyrogallol>guaiacol and was found to be a homotetramer of 155kDa with each subunit having a size of 38kDa. The basis of the distinctive preferences for various substrates was investigated through in-silico molecular modeling approaches. Thus, when the modeled papaya peroxidase-heme complex was docked with these substrates, the in-silico binding efficiency was found to be in agreement with those of wet lab results with the involvement of Arg37, Phe40, His41, Pro137, Asn138, His139, His167, and Phe239 as the common interacting residues in all the cases. However, the binding of the different substrates were found to be associated with conformational changes in the peroxidase. Thus, in the case of o-dianisidine (the most efficient substrate), the protein was folded in the most compact fashion when compared to guaiacol (the least efficient substrate). Protein function annotation analyses revealed that the papaya peroxidase may have biological roles in oxidation-reduction processes, stresses, defense responses etc. In order to further validate its role in lignifications, the papaya peroxidase was compared with a lignin biosynthetic peroxidase from Leucaena leucocephala, a tree legume. Thus, based on 3D structure superimposition and docking, both peroxidases exhibited a great extent of similarity suggesting the papaya peroxidase having a role in lignification (defense response) too. The predicted functions of papaya peroxidase in defense response and lignification were further validated experimentally using qRT-PCR analyses and measurement of oxidation of coniferyl alcohol. PMID:25447898

  6. Ficus carica L. (Moraceae): Phytochemistry, Traditional Uses and Biological Activities.

    PubMed

    Mawa, Shukranul; Husain, Khairana; Jantan, Ibrahim

    2013-01-01

    This paper describes the botanical features of Ficus carica L. (Moraceae), its wide variety of chemical constituents, its use in traditional medicine as remedies for many health problems, and its biological activities. The plant has been used traditionally to treat various ailments such as gastric problems, inflammation, and cancer. Phytochemical studies on the leaves and fruits of the plant have shown that they are rich in phenolics, organic acids, and volatile compounds. However, there is little information on the phytochemicals present in the stem and root. Reports on the biological activities of the plant are mainly on its crude extracts which have been proven to possess many biological activities. Some of the most interesting therapeutic effects include anticancer, hepatoprotective, hypoglycemic, hypolipidemic, and antimicrobial activities. Thus, studies related to identification of the bioactive compounds and correlating them to their biological activities are very useful for further research to explore the potential of F. carica as a source of therapeutic agents. PMID:24159359

  7. Ficus carica L. (Moraceae): Phytochemistry, Traditional Uses and Biological Activities

    PubMed Central

    Mawa, Shukranul; Husain, Khairana; Jantan, Ibrahim

    2013-01-01

    This paper describes the botanical features of Ficus carica L. (Moraceae), its wide variety of chemical constituents, its use in traditional medicine as remedies for many health problems, and its biological activities. The plant has been used traditionally to treat various ailments such as gastric problems, inflammation, and cancer. Phytochemical studies on the leaves and fruits of the plant have shown that they are rich in phenolics, organic acids, and volatile compounds. However, there is little information on the phytochemicals present in the stem and root. Reports on the biological activities of the plant are mainly on its crude extracts which have been proven to possess many biological activities. Some of the most interesting therapeutic effects include anticancer, hepatoprotective, hypoglycemic, hypolipidemic, and antimicrobial activities. Thus, studies related to identification of the bioactive compounds and correlating them to their biological activities are very useful for further research to explore the potential of F. carica as a source of therapeutic agents. PMID:24159359

  8. HOMOPTERAN AND MITE PESTS OF PAPAYA AND THEIR CONTROL

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Homopteran pests in papaya fields in Florida, include mealybugs, Paracoccus marginatus Williams and Granara de Willink, soft scales Philephedra tuberculosa Nakahara and Gill, Coccus hesperidum L. and armored scales, Aspidiotus destructor, Acutaspis sp., Hemiberlesia sp., aphids, Myzus persicae (Sul...

  9. 212 Florida Entomologist 89(2) June 2006 CLASSICAL BIOLOGICAL CONTROL OF THE PAPAYA MEALYBUG,

    E-print Network

    Reddy, Gadi VP

    and was causing serious damage to papaya, plume- ria, hibiscus, and other plants. The parasitoids Anagyrus loecki en papaya, hibiscus y otras plan- tas. Un total de 24,586 de los parasitoides,Anagyrus loecki Noyes

  10. Purification and autolysis of the ficin isoforms from fig (Ficus carica cv. Sabz) latex.

    PubMed

    Zare, Hamid; Moosavi-Movahedi, Ali Akbar; Salami, Maryam; Mirzaei, Morteza; Saboury, Ali Akbar; Sheibani, Nader

    2013-03-01

    Ficin (EC 3.4.22.3), a cysteine endoproteolytic protease in fig trees' latex, has multiple isoforms. Until now, no data on autolysis of individual ficins (ficin isoforms) are available. Following purification, ficins' autolysis was determined by HPLC chromatogram changes and ultrafiltrations at different temperatures and storage times. These results showed that the number of HPLC peaks in latex proteins purification of Ficus carica cv. Sabz varied from previous fig varieties or cultivars. Proteolytic activity of ficins was inhibited by specific cysteine protease inhibitors, confirming the participation of the cysteine residue in the active site. The zeta potential of the first two eluted peaks (I and II) was negative, while that of other peaks were positive. All ficins were susceptible to autolysis when stored at high temperatures. In contrast, only the last two ficins (B, C) were prone to autolysis at cold temperature after long storage period. The rate of degradation of the ficins was significantly increased with the increased storage time. The ficin (A) related to peak (III) had the highest and the lowest surface hydrophobic patches and ratio of autolytic to proteolytic activity, respectively. PMID:23312458

  11. 7 CFR 319.56-25 - Papayas from Central America and Brazil.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...false Papayas from Central America and Brazil. 319.56-25 Section 319.56-25...56-25 Papayas from Central America and Brazil. The Solo type of papaya may be...one of the following locations: (1) Brazil: State of Espirito...

  12. Ubiquitous presence of fastidious endophytic bacteria in field shoots and index-negative apparently clean shoot-tip cultures of papaya.

    PubMed

    Thomas, Pious; Kumari, Sima; Swarna, Ganiga K; Prakash, Devalakere P; Dinesh, Makki R

    2007-09-01

    This study establishes the widespread prevalence of fastidious or viable but non-culturable endophytic bacteria in field shoots and in unsuspicious shoot-tip cultures of papaya (Carica papaya L.) against the norm of asepsis in vitro. A total of 150 shoot-tips (approximately 10 mm) were inoculated on MS-based culture medium after surface sterilization of field-derived axillary shoots of cv. Surya during November or January (100 and 50, respectively) when 35-50% cultures showed endophytic microbial growth on culture medium. Indexing of apparently clean cultures using bacteriological media helped in detecting and removing additional 14-17% stocks with covert bacteria during the first two passages. The rest of the stocks stayed consistently index-negative during the first eight subculture cycles, but appeared positive in PCR-screening undertaken thereafter employing universal bacterial 16S rRNA gene primers indicating the association of non-cultivable bacteria. Direct sequencing of the PCR product yielded overlapping nucleotide data signifying mixed template or the presence of diverse endophytic microorganisms. This was confirmed by light microscopy of tissue sap revealing viable bacteria in considerable numbers, which were detected under phase contrast or with negative staining. Planting tissue segments or applying homogenate from these stocks on diverse bacteriological media did not induce the organisms to grow in vitro. The shoot cultures displayed variation in growth and rooting potential, the onus of such variation was solely attributable to the associated microorganisms. The findings were confirmed with additional field shoots and fresh in vitro stocks established subsequently. The observations have implications in micropropagation and all other applications involving plant cell, tissue, organ, and protoplast culture. PMID:17492452

  13. Immunotoxicity activity of natural furocoumarins from milky sap of Ficus carica L. against Aedes aegypti L.

    PubMed

    Chung, Iii-Min; Kim, Sun-Jin; Yeo, Min-A; Park, Se-Won; Moon, Hyung-In

    2011-09-01

    Ficus carica L., its fruits are delicious and can be eaten by human. Its leaves are commonly used to cure hemorrhoid and clear away heart ache. The milky sap of F. carica have a significant toxic effect against early fourth-stage larvae of Aedes aegypti L with an lethal concentration (LC(50)) value of 10.2??g/ml and an LC(90) value of 42.3??g/ml. Two natural furocoumarins, 5-methoxypsoralen and 8-methoxypsoralen were isolated from the milky sap of F. carica. The LC(50) value of 5-methoxypsoralen and 8-methoxypsoralen were 9.4 and 56.3??g/ml, respectively. The above indicates that major compounds may play a more important role in the toxicity of the milky sap of F. carica. PMID:21214422

  14. Sequence and genome organization of papaya meleira virus infecting papaya in Brazil.

    PubMed

    Abreu, Emanuel F M; Daltro, Cleidiane B; Nogueira, Elsa O P L; Andrade, Eduardo C; Aragão, Francisco J L

    2015-12-01

    Papaya sticky disease ('meleira') was first observed in Brazil at the beginning of the 1980s. The disease is characterized by intense latex exudation from the fruit surface that becomes dark as it oxidizes, which makes it difficult to sell. The causal agent, which has been called papaya meleira virus (PMeV), has been identified as an isometric virus particle, approximately 50 nm in diameter, with a double-stranded RNA genome. Here, we report the first complete sequence and organization of the 8.7-kb viral dsRNA genome. Two ORFs coding for a putative coat protein and RNA-dependent RNA polymerase (RdRp) were predicted. In silico analysis revealed that the translated ORF2 contains the conserved domains characteristic of an RdRp protein (pfam02123:RdRP 4), which is a family that includes RdRps from members of the genera Luteovirus, Totivirus and Rotavirus. Evolutionary analysis with amino acid sequences with the RdRps from members of the family Totiviridae and some dsRNA viruses showed that PMeV RdRp did not root itself in any genus. PMID:26370790

  15. Consumer in-store response to irradiated papayas

    SciTech Connect

    Bruhn, C.M.; Noell, J.W.

    1987-09-01

    In this study, purchase behavior of California consumers in response to irradiated papayas is described. The papayas were shipped from Hawaii and irradiated in California under a permit by the Animal and Plant Health Inspection Service and approved by the California Department of Food and Agriculture. Results show that the superior appearance of the irradiated fruit appealed to consumers and that two-thirds or more of the people queried indicated that they would buy irradiated produce. It is noted that this marketing took place in a supportive environment with no protestors present. Informational material was available.

  16. Identification of phenylpropanoids in fig (Ficus carica L.) leaves.

    PubMed

    Takahashi, Toru; Okiura, Aya; Saito, Keita; Kohno, Masahiro

    2014-10-15

    In this study, the phenylpropanoid composition and antioxidant activity of identified components in fig (Ficus carica L.) leaves were examined. Known polyphenols rutin, isoschaftoside, isoquercetin, and chlorogenic acid were identified. Furthermore, caffeoylmalic acid (CMA) was the most abundant polyphenol and was identified for the first time. CMA exhibited antioxidant activity similar to that of vitamin C or catechin. Psoralen and bergapten were identified as known furanocoumarins, with psoralen being the most abundant. Moreover, psoralic acid glucoside (PAG) was identified for the first time. As a precursor of psoralen, PAG content was equivalent to the psoralen content in moles. Notably, the content of these compounds varied between the five fig varieties, and the furanocoumarin and PAG contents varied more than that of the polyphenols. Further investigations concerning the influence of CMA and PAG on human health are necessary to elucidate functionalities of fig leaves. PMID:25198243

  17. 75 FR 22207 - Importation of Papayas From Colombia and Ecuador

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-04-28

    ... published in the Federal Register (74 FR 18161-18166, Docket No. APHIS-2008-0050) a proposal\\1\\ to amend the... field sanitation, hot water treatment, and fruit fly trapping in papaya production areas. This action... quarantine pests, and that fruit fly trapping, field sanitation, and hot water treatment be employed...

  18. 78 FR 48628 - Importation of Papayas From Peru

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ... papayas from Peru would include requirements for approved production locations; field sanitation; hot... sanitation, and hot water treatment to remove pests of concern from the pathway. Only commercial consignments... hot water dip treatment discussed later in this document is effective, as that treatment has not...

  19. Papaya fruit quality management during the postharvest supply chain

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papayas are popular in tropical and subtropical regions and are being exported in large volumes to Europe, the U.S. and Japan. The fruit has excellent taste, exotic flavor and nutritional properties, being rich in vitamins A, C, and antioxidants. However, due to its highly perishable nature it has n...

  20. DIFFERENTIAL EXPRESSION OF PAPAYA PROTEIN PROFILE USING PROTEOMIC TOOLS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The objective of our project is to evaluate the potential for using proteomic approaches to discover the basis for differential susceptibility of Hawaii papaya cultivars to the root rot pathogen Phytophthora palmivora. Based on previous studies showing that Kapoho was the most resistance cultivar an...

  1. 78 FR 48628 - Importation of Papayas From Peru

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-08-09

    ...be held for 20 minutes in hot water at 48 [deg]C (118.4...in Sec. 319.56-25. Hot water treatment mitigates the pest...would allow the importation of fresh papaya fruit from Peru into...20250. A comment to OMB is best assured of having its full...

  2. Effects of Cream Containing Ficus carica L. Fruit Extract on Skin Parameters: In vivo Evaluation.

    PubMed

    Khan, H; Akhtar, N; Ali, A

    2014-01-01

    This study was aimed to investigate the effects of cream containing Ficus carica L. fruit (Fig) extract on various skin parameters such as skin melanin, erythema, moisture content, trans-epidermal water loss and sebum. For this purpose, formulation with 4% concentrated extract of F. carica fruit and base without extract were developed. Base served as a control. Both base and formulation were applied to the cheeks of human volunteers for 8 weeks to investigate the effects on different skin parameters using non-invasive bioengineering instruments. Formulation decreased the skin melanin, trans-epidermal water loss and skin sebum significantly. Formulation increased the skin hydration significantly and insignificant effects on skin erythema. We concluded that a stable topical cream (w/o emulsion) containing F. carica fruit extract have effects on skin melanin, trans-epidermal loss, hydration values and sebum content and possibly could be used against for hyper pigmentation, acne, freckles and wrinkle. PMID:25593393

  3. Anti-inflammatory and antioxidant activity of Ficus carica Linn. leaves.

    PubMed

    Ali, B; Mujeeb, M; Aeri, V; Mir, S R; Faiyazuddin, M; Shakeel, F

    2012-01-01

    Ficus carica Linn. (Moraceae) is commonly known as edible fig. The leaves, roots, fruits and latex of the plant are medicinally used in different diseases. The leaves are claimed to be effective in various inflammatory conditions like painful or swollen piles, insect sting and bites. However, there has been no report on anti-inflammatory and antioxidant activity of F. carica leaves. Therefore the aim of this study was to evaluate the anti-inflammatory and antioxidant activity of F. carica leaves. Our study validated the traditional claim with pharmacological data. Anti-inflammatory and antioxidant activity of the drug could be due to the presence of steroids and flavanoids, respectively, which are reported to be present in the drug. Furthermore, the anti-inflammatory activity of the drug could be due to its free radical scavenging activity. Further work is also required to isolate and characterise the active constituents responsible for the anti-inflammatory activities. PMID:21644169

  4. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  5. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  6. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  7. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  8. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Coat Protein of Papaya Ringspot Virus...-INCORPORATED PROTECTANTS Tolerances and Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  9. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...2013-07-01 2013-07-01 false Coat Protein of Papaya Ringspot Virus; exemption...Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption...requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  10. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...2011-07-01 2011-07-01 false Coat Protein of Papaya Ringspot Virus; exemption...Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption...requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  11. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...2014-07-01 2014-07-01 false Coat Protein of Papaya Ringspot Virus; exemption...Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption...requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  12. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...2012-07-01 2012-07-01 false Coat Protein of Papaya Ringspot Virus; exemption...Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption...requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  13. 40 CFR 174.515 - Coat Protein of Papaya Ringspot Virus; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Coat Protein of Papaya Ringspot Virus; exemption...Tolerance Exemptions § 174.515 Coat Protein of Papaya Ringspot Virus; exemption...requirement of a tolerance. Residues of Coat Protein of Papaya Ringspot Virus are...

  14. Assaying for pollen drift from transgenic Rainbow to nontransgenic Kapoho papaya under commercial and experimental field conditions in Hawaii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In 1992 papaya ringpsot virus (PRSV) was discovered in Puna district of Hawaii Island where 95% of the state of Hawaii’s papaya was being grown. By 1998 production in Puna had decreased 50% from 1998 levels. A PRSV-resistant transgenic papaya ‘Rainbow’ expressing the coat protein gene of PRSV was ...

  15. Ficus carica L. (Moraceae): an ancient source of food and health.

    PubMed

    Barolo, Melisa I; Ruiz Mostacero, Nathalie; López, Silvia N

    2014-12-01

    Since early in the man history, common fig was appreciated as food and for its medicinal properties. This review explores some aspects about the importance of Ficus carica L., an amazing and ancient source of medicines and food. Topics regarding chemistry, biological activity, ethno-pharmacological uses, and its nutritional value are discussed, as well as the potential of the species as a source of new and different chemical scaffolds. Very important in the past, appreciated in our time and extremely promising in the future, F. carica represents an interesting example of healthy foods and bioproducts. PMID:24996314

  16. Phenological Adaptations in Ficus tikoua Exhibit Convergence with Unrelated Extra-Tropical Fig Trees

    PubMed Central

    Zhao, Ting-Ting; Compton, Stephen G.; Yang, Yong-Jiang; Wang, Rong; Chen, Yan

    2014-01-01

    Flowering phenology is central to the ecology and evolution of most flowering plants. In highly-specific nursery pollination systems, such as that involving fig trees (Ficus species) and fig wasps (Agaonidae), any mismatch in timing has serious consequences because the plants must balance seed production with maintenance of their pollinator populations. Most fig trees are found in tropical or subtropical habitats, but the dioecious Chinese Ficus tikoua has a more northerly distribution. We monitored how its fruiting phenology has adapted in response to a highly seasonal environment. Male trees (where fig wasps reproduce) had one to three crops annually, whereas many seed-producing female trees produced only one fig crop. The timing of release of Ceratosolen fig wasps from male figs in late May and June was synchronized with the presence of receptive figs on female trees, at a time when there were few receptive figs on male trees, thereby ensuring seed set while allowing remnant pollinator populations to persist. F. tikoua phenology has converged with those of other (unrelated) northern Ficus species, but there are differences. Unlike F. carica in Europe, all F. tikoua male figs contain male flowers, and unlike F. pumila in China, but like F. carica, it is the second annual generation of adult wasps that pollinate female figs. The phenologies of all three temperate fig trees generate annual bottlenecks in the size of pollinator populations and for female F. tikoua also a shortage of fig wasps that results in many figs failing to be pollinated. PMID:25474008

  17. Phenological adaptations in Ficus tikoua exhibit convergence with unrelated extra-tropical fig trees.

    PubMed

    Zhao, Ting-Ting; Compton, Stephen G; Yang, Yong-Jiang; Wang, Rong; Chen, Yan

    2014-01-01

    Flowering phenology is central to the ecology and evolution of most flowering plants. In highly-specific nursery pollination systems, such as that involving fig trees (Ficus species) and fig wasps (Agaonidae), any mismatch in timing has serious consequences because the plants must balance seed production with maintenance of their pollinator populations. Most fig trees are found in tropical or subtropical habitats, but the dioecious Chinese Ficus tikoua has a more northerly distribution. We monitored how its fruiting phenology has adapted in response to a highly seasonal environment. Male trees (where fig wasps reproduce) had one to three crops annually, whereas many seed-producing female trees produced only one fig crop. The timing of release of Ceratosolen fig wasps from male figs in late May and June was synchronized with the presence of receptive figs on female trees, at a time when there were few receptive figs on male trees, thereby ensuring seed set while allowing remnant pollinator populations to persist. F. tikoua phenology has converged with those of other (unrelated) northern Ficus species, but there are differences. Unlike F. carica in Europe, all F. tikoua male figs contain male flowers, and unlike F. pumila in China, but like F. carica, it is the second annual generation of adult wasps that pollinate female figs. The phenologies of all three temperate fig trees generate annual bottlenecks in the size of pollinator populations and for female F. tikoua also a shortage of fig wasps that results in many figs failing to be pollinated. PMID:25474008

  18. An umbra-like virus of papaya discovered in Ecuador: detection, occurrence and phylogenetic relatedness

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Double-stranded RNA (dsRNA) extractions from papaya leaves infected with Papaya ringspot virus (PRSV) revealed the presence of an unusual 4kb band, in addition to the presumed PRSV-associated 10kb band. Partial sequence of RT-PCR products from the 4kb dsRNA revealed homology to genomes of several me...

  19. TISSUE DIFFERENTIAL EXPRESSION OF LYCOPENE BETA-CYCLASE GENE IN PAPAYA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    To elucidate the carotenoid biosynthesis pathway in papaya, we took a candidate gene approach to clone the lycopene beta-cyclase genes, LCY-B. A papaya LCY-B ortholog, cpLCY-B, was successfully identified from both cDNA and bacterial artificial chromosome (BAC) libraries and complete genomic sequenc...

  20. Cloning Of Organ-Specific Genes from Papaya Using cDNA-AFLP

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya is a fast growing and productive fruit crop in tropical and subtropical regions worldwide. Papaya industry has benefited from application of molecular biology and biotechnology for crop improvement, particularly for viral disease resistance using transgenic varieties. The goal of this researc...

  1. A Current Overview of the Papaya meleira virus, an Unusual Plant Virus

    PubMed Central

    Abreu, Paolla M. V.; Antunes, Tathiana F. S.; Magaña-Álvarez, Anuar; Pérez-Brito, Daisy; Tapia-Tussell, Raúl; Ventura, José A.; Fernandes, Antonio A. R.; Fernandes, Patricia M. B.

    2015-01-01

    Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease, which is characterized by a spontaneous exudation of fluid and aqueous latex from the papaya fruit and leaves. The latex oxidizes after atmospheric exposure, resulting in a sticky feature on the fruit from which the name of the disease originates. PMeV is an isometric virus particle with a double-stranded RNA (dsRNA) genome of approximately 12 Kb. Unusual for a plant virus, PMeV particles are localized on and linked to the polymers present in the latex. The ability of the PMeV to inhabit such a hostile environment demonstrates an intriguing interaction of the virus with the papaya. A hypersensitivity response is triggered against PMeV infection, and there is a reduction in the proteolytic activity of papaya latex during sticky disease. In papaya leaf tissues, stress responsive proteins, mostly calreticulin and proteasome-related proteins, are up regulated and proteins related to metabolism are down-regulated. Additionally, PMeV modifies the transcription of several miRNAs involved in the modulation of genes related to the ubiquitin-proteasome system. Until now, no PMeV resistant papaya genotype has been identified and roguing is the only viral control strategy available. However, a single inoculation of papaya plants with PMeV dsRNA delayed the progress of viral infection. PMID:25856636

  2. DEVELOPMENT, REPRODUCTION, AND SURVIVAL OF PAPAYA MEALYBUG (HOMOPTERA: PSEUDOCOCCIDAE) ON DIFFERENT HOST PLANT SPECIES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Papaya mealybug (Paracoccus marginatus @illiams and Granara de Willink (Homoptera: Pseudococcidae)) is a polyghagus insect and a pest of various tropical crops and ornamentals. It was introduced into the United States in 1998 in Florida. Papaya mealybug potentially poses a threat to numerous agricul...

  3. Di Volta in volta in citt L'Alessandro che ci ha dato la "carica"

    E-print Network

    Gilardi, Gianni

    Di Volta in volta in città L'Alessandro che ci ha dato la "carica" Sabato 28 Marzo Il Museo per la propongono un percorso "strada e museo" alla scoperta del cittadino Alessandro Volta h. 15,00 ­ ritrovo all'angolo tra Via Volta e Via Scopoli h. 16,00 ­ Museo per la Storia dell'Università Palazzo Centrale, Strada

  4. VITAMIN AND MINERAL CONTENT OF TROPICAL FRUIT CULTIVARS GROWN IN HAWAII

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Banana (Musa sp.), papaya (Carica papaya), longan (Dimocarpus longan), lychee (Litchi chinensis), and rambutan (Nephelium lappaceum) cultivars were harvested from different locations throughout Hawaii and analyzed for vitamin C (ascorbic acid) and mineral content. Bananas and papayas also were analy...

  5. Nursery, Landscape & Ornamentals Stem cutting propagation of fig trees (Ficus carica)

    E-print Network

    Chen, Tsuhan

    pollinating insects in order to produce large numbers of fruit. The plants grow well in a well-drained soil-value, unique fruit for farmers markets and farm stands. Although the plants are easy to grow, the difficulty knife. A commercial rooting powder with 0.1% indole-3 butyric Acid (IBA) was applied to the base

  6. Tree Location One, Two, Tree!

    E-print Network

    Ashline, George

    Tree Location One, Two, Tree! Let's Learn about the Linden! A tree activity for little Seedlings colors on a piece of paper. What are the parts of the tree? 1.) See if you can name all the different parts of the tree. 2.) Write down the parts on a piece of paper. What do you see in, on, or around

  7. Phospholipid Profile of the Stomach and Duodenum of Normal Rabbits Fed with Supplements of Unripe Pawpaw (Carica papaya) and Unripe Plantain (Musa sapientum) Extract

    NASA Astrophysics Data System (ADS)

    Eriyamremu, G. E.; Asagba, S. O.; Osagie, V. E.; Ojeaburu, S. I.; Lolodi, O.

    This study reports the effect of unripe pawpaw and/or unripe plantain extracts on the phospholipid profile of the stomach and duodenum of normal rabbits. Supplementation of chow with unripe pawpaw or plantain extract significantly reduced (p< 0.05) the weight gained by rabbits compared with the control. The total phospholipids content in the stomach and duodenum were significantly increased (p< 0.05) in the test groups compared to the control. In the stomach and duodenum, phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SGM) were significantly increased (p< 0.05) in the test groups compared to the control. Pawpaw occasioned a decrease in the PE/PC ratio in both tissues as against an increase observed in the SGM/PC ratio. The results presented suggests that unripe pawpaw meal and unripe plantain extract alter the phospholipid profile of the stomach and duodenum in ways which may affect membrane fluidity of these tissues and would have profound effect on the gastro-duodenal mucosa and thus have implication(s) for gastric and duodenal ulcers in rabbits.

  8. Identification and quantification of furanocoumarins in stem bark and wood of eight Algerian varieties of Ficus carica by RP-HPLC-DAD and RP-HPLC-DAD-MS.

    PubMed

    Rouaiguia-Bouakkaz, Samia; Amira-Guebailia, Habiba; Rivière, Céline; Delaunay, Jean-Claude; Waffo-Téguo, Pierre; Mérillon, Jean-Michel

    2013-04-01

    Furanocoumarins are the major phytoalexins of Ficus carica and are effective natural drug candidates for treatment of several types of cancer and skin disease. The objectives of this study were to analyze and quantify linear furanocoumarins, mainly psoralen and bergapten, in wood and bark of stems from eight Algerian varieties of fig and to establish the differences in the content of these metabolites in the eight local samples. Psoralen and bergapten contents in the stem bark and wood (in microg/g DW) varied respectively from 146.6 to 1110.3 and from 395.7 to 1671.8 for psoralen, and from 114.3 to 524.0 and from 144.2 to 718.6 for bergapten. This study fills a gap in our knowledge of furanocoumarin distribution in different parts of the fig tree. Psoralen and bergapten concentrations were higher in the wood than in the stem bark. Most of the dark fruited fig trees produce these two coumarins more than the green ones. PMID:23738460

  9. Flower Face Face Face Face Flower Tree Tree Tree Tree

    E-print Network

    Chen, Tsuhan

    Flower Flower Flower Flower Flower Face Face Face Face Flower Flower Tree Tree Tree Tree Flower Flower Flower Flower Sky Sky Sky Sky Flower Grass Grass Grass Grass Flower Flower Flower #12;Sign Sign Road Road Chair Face Face Face Face Chair #12;Chair Chair Chair Flower Flower Flower Flower Grass Chair

  10. New furanocoumarins and other chemical constituents from Ficus carica root heartwood.

    PubMed

    Jain, Renuka; Jain, Satish C; Bhagchandani, Teena; Yadav, Namita

    2013-01-01

    Two new furanocoumarins, 5-(1",1"-dimethylallyl)-8-methyl psoralen (1) and 2"-O-acetyl oxypeucedanin hydrate-3"-methyl ether (2), were isolated from the root heartwood of Ficus carica Linn. together with three known furanocoumarins, two triterpenoids, two long-chain compounds, and a steroid. Their structures and relative configurations were elucidated by spectroscopic methods (IR, HR-ESI-MS, and NMR) and by comparison of their NMR spectral data with those of related compounds. PMID:23659166

  11. Ficus carica Polysaccharides Promote the Maturation and Function of Dendritic Cells

    PubMed Central

    Tian, Jie; Zhang, Yue; Yang, Xiaomin; Rui, Ke; Tang, Xinyi; Ma, Jie; Chen, Jianguo; Xu, Huaxi; Lu, Liwei; Wang, Shengjun

    2014-01-01

    Various polysaccharides purified from plants are considered to be biological response modifiers and have been shown to enhance immune responses. Ficus carica L. is a Chinese traditional plant and has been widely used in Asian countries for its anti-tumor properties. Ficus carica polysaccharides (FCPS), one of the most essential and effective components in Ficus carica L., have been considered to be a beneficial immunomodulator and may be used in immunotherapy. However, the immunologic mechanism of FCPS is still unclear. Dectin-1 is a non-toll-like pattern recognition receptor, predominately expressed on dendritic cells (DCs). Activation of DCs through dectin-1 signaling can lead to the maturation of DC, thus inducing both innate and adaptive immune responses against tumor development and microbial infection. In our study, we found that FCPS could effectively stimulate DCs, partially through the dectin-1/Syk pathway, and promote their maturation, as shown by the up-regulation of CD40, CD80, CD86, and major histocompatibility complex II (MHCII). FCPS also enhanced the production of cytokines by DCs, including IL-12, IFN-?, IL-6, and IL-23. Moreover, FCPS-treated DCs showed an enhanced capability to stimulate T cells and promote T cell proliferation. Altogether, these results demonstrate that FCPS are able to activate and maturate DCs, thereby up-regulating the immunostimulatory capacity of DCs, which leads to enhanced T cell responses. PMID:25026176

  12. Ficus carica polysaccharides promote the maturation and function of dendritic cells.

    PubMed

    Tian, Jie; Zhang, Yue; Yang, Xiaomin; Rui, Ke; Tang, Xinyi; Ma, Jie; Chen, Jianguo; Xu, Huaxi; Lu, Liwei; Wang, Shengjun

    2014-01-01

    Various polysaccharides purified from plants are considered to be biological response modifiers and have been shown to enhance immune responses. Ficus carica L. is a Chinese traditional plant and has been widely used in Asian countries for its anti-tumor properties. Ficus carica polysaccharides (FCPS), one of the most essential and effective components in Ficus carica L., have been considered to be a beneficial immunomodulator and may be used in immunotherapy. However, the immunologic mechanism of FCPS is still unclear. Dectin-1 is a non-toll-like pattern recognition receptor, predominately expressed on dendritic cells (DCs). Activation of DCs through dectin-1 signaling can lead to the maturation of DC, thus inducing both innate and adaptive immune responses against tumor development and microbial infection. In our study, we found that FCPS could effectively stimulate DCs, partially through the dectin-1/Syk pathway, and promote their maturation, as shown by the up-regulation of CD40, CD80, CD86, and major histocompatibility complex II (MHCII). FCPS also enhanced the production of cytokines by DCs, including IL-12, IFN-?, IL-6, and IL-23. Moreover, FCPS-treated DCs showed an enhanced capability to stimulate T cells and promote T cell proliferation. Altogether, these results demonstrate that FCPS are able to activate and maturate DCs, thereby up-regulating the immunostimulatory capacity of DCs, which leads to enhanced T cell responses. PMID:25026176

  13. Oral dosing with papaya latex is an effective anthelmintic treatment for sheep infected with Haemonchus contortus

    PubMed Central

    2011-01-01

    Background The cysteine proteinases in papaya latex have been shown to have potent anthelmintic properties in monogastric hosts such as rodents, pigs and humans, but this has not been demonstrated in ruminants. Methods In two experiments, sheep were infected concurrently with 5,000 infective larvae of Haemonchus contortus and 10,000 infective larvae of Trichostrongylus colubriformis and were then treated with the supernatant from a suspension of papaya latex from day 28 to day 32 post-infection. Faecal egg counts were monitored from a week before treatment until the end of the experiment and worm burdens were assessed on day 35 post-infection. Results We found that the soluble fraction of papaya latex had a potent in vivo effect on the abomasal nematode H. contortus, but not on the small intestinal nematode T. colubriformis. This effect was dose-dependent and at tolerated levels of gavage with papaya latex (117 ?mol of active papaya latex supernatant for 4 days), the H. contortus worm burdens were reduced by 98%. Repeated treatment, daily for 4 days, was more effective than a single dose, but efficacy was not enhanced by concurrent treatment with the antacid cimetidine. Conclusions Our results provide support for the idea that cysteine proteinases derived from papaya latex may be developed into novel anthelmintics for the treatment of lumenal stages of gastro-intestinal nematode infections in sheep, particularly those parasitizing the abomasum. PMID:21406090

  14. Production of Internal Yellowing Symptoms on Resistant and Susceptible Papaya Cultivars by Enterobacter cloacae at Varying Inoculum Concentrations

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Internal yellowing (IY) is a bacterial disease of ripening papaya flesh caused by Enterobacter cloacae and characterized by yellow softening tissue. IY restricts food safety of value-added products like fresh or frozen papaya cubes. The incidence of E. cloacae presumably differs in resistant (R) a...

  15. TREE TRAILS Tree Scavenger Hunt

    E-print Network

    _______________________ Birdhouse _______________________ Food packaging _______________________ Chocolate) Food packaging - pulp Chocolate - nut (from cacao tree) Charcoal - wood Expert Items: Find it = 3

  16. Carbon disulfide formation in papaya under conditions of dithiocarbamate residue analysis.

    PubMed

    Abakerli, R B; Sparrapan, R; Sawaya, A C H F; Eberlin, M N; Jara, J L P; Rodrigues, N R; Fay, E F; Luiz, A J B; Galvão, T D L; Martins, D dos S; Yamanishi, O K; Toledo, H H B

    2015-12-01

    Golden, Sunrise Solo and Tainung cultivars of papaya were found to release CS2 when submitted to experimental conditions of dithiocarbamate residue analysis. Three common analytical methods were used to quantitate CS2; one spectrophotometric method and two chromatographic methods. All three methods gave positive CS2 results for all three papaya varieties. Other endogenous compounds present in isooctane extracts of papaya fractions detected via gas chromatography (GC/ITD) using electron ionization (EI) were: carbonyl sulfide, dimethyl sulfide, carbon disulfide, 2-methylthiophene, 3-methylthiophene, 2-ethylthiophene, 3-ethylthiophene, benzylisothiocyanate, benzylthiocyanate and benzonitrile. Control samples were obtained from papaya plantations cultivated in experimental areas, in which no treatment with fungicides of the dithiocarbamate group was applied. Endogenous CS2 levels were compared with true dithiocarbamate residues measured in papaya samples from the field trials following applications of the mancozeb fungicide. Three days after application, true dithiocarbamate residues, measured by the procedure with isooctane partitioning and GC-ITD, were at the average level of 2 mg kg(-1). PMID:26041166

  17. Characterization of the mucilage extracted from jaracatiá (Carica quercifolia (A. St. Hil.) Hieron).

    PubMed

    Faccio, Carina; Machado, Ricardo A F; de Souza, Lauro M; Zoldan, Sérgio R; Quadri, Mara G N

    2015-10-20

    The mucilage of the jaracatiá fruit (Carica quercifolia (A. St. Hil.) Hieron) was extracted and for physicochemical characterization. The monosaccharide composition showed the presence of Rha, Ara, Xyl, Gal, Glc and GalA, being confirmed by GC-MS, FTIR and NMR. The mucilage was obtained in crude form by lyophilization of the extract and by precipitation, a process that resulted in a partial purification. Although not remarkable, it showed an antioxidant and antimicrobial potential. The thermogravimetric analysis indicated an easy handling at temperatures below 250°C. The natural reactivity of the material indicates for uses such as adsorbent or raw material for membranes. PMID:26256196

  18. Tirucallane-type triterpenoids from the fruit of Ficus carica and their cytotoxic activity.

    PubMed

    Jing, Lin; Zhang, Yang-Mei; Luo, Jian-Guang; Kong, Ling-Yi

    2015-01-01

    Nine new tirucallane-type triterpenoids, ficutirucins A-I (1-9), were isolated from the fruit of Ficus carica. Their structures were established on the basis of spectroscopic data and chemical methods. All isolates were evaluated for their cytotoxic activities against three human cancer cell lines, MCF-7, HepG-2, and U2OS. Compounds 1-3, 6, 7, and 9 exhibited moderate cytotoxic activities with IC50 values of 11.67-45.61?µM against one or more of the three cancer cell lines. PMID:25757495

  19. Comparative activity of antioxidants from wheat sprouts, Morinda citrifolia, fermented papaya and white tea.

    PubMed

    Calzuola, Isabella; Gianfranceschi, Gian Luigi; Marsili, Valeria

    2006-01-01

    Hydroalcoholic extracts from wheat sprouts, white tea, Morinda citrifolia and fermented papaya were analysed to determine their reducing power and antioxidant activity. The results show that the micromoles of potassium ferricyanide reduced by a quantity of extract corresponding to 1 g of the various dehydrated starting tissues are: 12.91+/-0.83 (wheat sprouts), 10.66+/-1.22 (M. citrifolia), 17.06+/-1.24 (white tea), and 1.05+/-0.09 (fermented papaya). In addition the results show a strong oxygen superoxide scavenging activity in the extracts from white tea, M. citrifolia and wheat sprouts. The activity of the fermented papaya extract is the lowest. The thin-layer chromatography and UV spectrophotometry of the extracts show in each source a mixture of antioxidant compounds probably belonging to the families of reducing glycosides and polyphenols. The chromatographic pattern of the antioxidant compounds and the UV spectrum are quite different in the various sources. PMID:17127467

  20. Stability and efficiency of dye-sensitized solar cells based on papaya-leaf dye

    NASA Astrophysics Data System (ADS)

    Suyitno, Suyitno; Saputra, Trisma Jaya; Supriyanto, Agus; Arifin, Zainal

    2015-09-01

    The present article reports on the enhancement of the performance and stability of natural dye-based dye-sensitized solar cells (DSSCs). Natural dyes extracted from papaya leaves (PL) were investigated as sensitizers in TiO2-based DSSCs and evaluated in comparison with N719 dye. The acidity of the papaya-leaf extract dyes was tuned by adding benzoic acid. The TiO2 film-coated fluorine-doped tin oxide glass substrates were prepared using the doctor-blade method, followed by sintering at 450 °C. The counter electrode was coated by chemically deposited catalytic platinum. The working electrodes were immersed in N719 dye and papaya dye solutions with concentrations of 8 g/100 mL. The absorbance spectra of the dyes were obtained by ultra-violet-visible spectroscopy. The energy levels of the dyes were measured by the method of cyclic voltammetry. In addition, Fourier transform infrared spectroscopy was used to determine the characteristic functionalities of the dye molecules. The DSSC based on the N719 dye displayed a highest efficiency of 0.87% whereas those based on papaya-leaf dye achieved 0.28% at pH 3.5. The observed improved efficiency of the latter was attributed to the increased current density value. Furthermore, the DSSCs based on papaya-leaf dye with pH 3.5-4 exhibited better stability than those based on N719 dye. However, further studies are required to improve the current density and stability of natural dye-based DSSCs, including the investigation of alternative dye extraction routes, such as isolating the pure chlorophyll from papaya leaves and stabilizing it.

  1. Evolutionary history and variation in host range of three Stagonosporopsis species causing gummy stem blight of cucurbits.

    PubMed

    Stewart, Jane E; Turner, Ashley N; Brewer, Marin T

    2015-05-01

    Recently diverged species may form complexes of morphologically similar, yet genetically distinct lineages that occur in overlapping geographic ranges and niches. Using a multilocus sequencing approach we discovered that gummy stem blight of cucurbits is caused by three genetically distinct species: Stagonosporopsis cucurbitacearum (syn. Didymella bryoniae), Stagonosporopsis citrulli, and Stagonosporopsis caricae, which had previously been considered only a pathogen of papaya. Experiments showed that all three species are pathogenic to cucurbits in the genera Cucurbita, Cucumis, and Citrullus, but only S. caricae is aggressive to papaya. Species tree estimates show that S. citrulli and S. cucurbitacearum are phylogenetically distinct sister species, and that S. caricae is the ancestral lineage. The time estimate for divergence of S. caricae from the ancestor of S. cucurbitacearum and S. citrulli at 72 900 YBP pre-dates domestication of papaya and Cucurbita species in the American tropics. The divergence estimate observed for S. cucurbitacearum and S. citrulli at 10 900 YBP suggests that diversification of Cucurbita species and domestication of gourds and squashes could have driven their divergence. This work highlights the use of molecular systematics and population genetics to elucidate genetic identity among previously unassociated fungi and to understand the patterns of pathogen diversification. PMID:25937065

  2. In vitro antiviral activity of Ficus carica latex against caprine herpesvirus-1.

    PubMed

    Camero, Michele; Marinaro, Mariarosaria; Lovero, Angela; Elia, Gabriella; Losurdo, Michele; Buonavoglia, Canio; Tempesta, Maria

    2014-01-01

    The latex of Ficus carica Linn. (Moraceae) has been shown to possess antiviral properties against some human viruses. To determine the ability of F. carica latex (F-latex) to interfere with the infection of caprine herpesvirus-1 (CpHV-1) in vitro, F-latex was resuspended in culture media containing 1% ethanol and was tested for potential antiviral effects against CpHV-1. Titration of CpHV-1 in the presence or in the absence of F-latex was performed on monolayers of Madin Darby Bovine Kidney (MDBK) cells. Simultaneous addition of F-latex and CpHV-1 to monolayers of MDBK cells resulted in a significant reduction of CpHV-1 titres 3 days post-infection and this effect was comparable to that induced by acyclovir. The study suggests that the F-latex is able to interfere with the replication of CpHV-1 in vitro on MDBK cells and future studies will determine the mechanisms responsible for the observed antiviral activity. PMID:24853920

  3. Characterization of fig achenes' oil of Ficus carica grown in Tunisia.

    PubMed

    Soltana, Hala; Tekaya, Meriem; Amri, Zahra; El-Gharbi, Sinda; Nakbi, Amel; Harzallah, Arij; Mechri, Beligh; Hammami, Mohamed

    2016-04-01

    This work investigated the composition of the oil extract from achenes of "Kholi" variety of Ficus carica, grown in Tunisia. Fatty acid and sterol compositions were analyzed by gas chromatography (GC) coupled to flame ionization detector (FID). Furthermore, the antioxidant capacity in fig achenes' oil was assessed by employing two different in vitro assays such as DPPH, ABTS(+) radical scavenging capacities. Our results indicated that the fig achenes' oil is a rich source of bioactive molecules. The soxhlet n-hexane extraction of these achenes produced a total oil yield of 16.24%. The predominant fatty acid was linolenic acid. Concerning phytosterols, the total amount reached 1061.45mg/100g with a predominance of ?(5,23)-stigmastadienol (73.78%). Regarding antioxidant activities, the half maximal inhibitory concentration (IC50) was 215.86?g/ml and Trolox equivalent antioxidant capacity (TEAC) was 95.25mM. These data indicate that fig achenes oil of F. carica could be potentially useful in food and pharmaceutical applications. PMID:26593597

  4. Semen study of papaya workers exposed to ethylene dibromide

    SciTech Connect

    Ratcliffe, J.M.; Schrader, S.M.; Steenland, K.; Clapp, D.; Turner, T.

    1984-01-01

    A cross sectional semen and cytogenetic study was performed on male workers exposed to ethylene-dibromide (EDB) in the papaya fumigation industry in Hawaii. Semen analyses were conducted on 46 men in six fumigation facilities with an average length of employment of 5 years and airborne exposures to EDB ranging from 16 to 213 parts per billion. Statistically significant decreases in sperm count per ejaculate and the percentage of viable and motile sperm and increases in the proportion of specific morphological abnormalities were observed among exposed men when compared with controls. Semen volume and sperm concentration were also lower in the exposed group. No effect of exposure to EDB on sperm velocity, the overall proportion of sperm with normal morphology or YFF bodies was noted. The authors conclude that based on the decreases in sperm count, viability and motility and increases in certain types of morphological abnormalities among workers exposed to EDB, EDB may increase the risk of reproductive impairment in workers at exposure levels near the NIOSH recommended limit of 45 parts per billion and far below the current OSHA standard of 20 parts per million.

  5. Tree Amigos.

    ERIC Educational Resources Information Center

    Center for Environmental Study, Grand Rapids, MI.

    Tree Amigos is a special cross-cultural program that uses trees as a common bond to bring the people of the Americas together in unique partnerships to preserve and protect the shared global environment. It is a tangible program that embodies the philosophy that individuals, acting together, can make a difference. This resource book contains…

  6. Talking Trees

    ERIC Educational Resources Information Center

    Tolman, Marvin

    2005-01-01

    Students love outdoor activities and will love them even more when they build confidence in their tree identification and measurement skills. Through these activities, students will learn to identify the major characteristics of trees and discover how the pace--a nonstandard measuring unit--can be used to estimate not only distances but also the…

  7. 7 CFR 319.56-25 - Papayas from Central America and Brazil.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... safeguarded from exposure to fruit flies from harvest to export, including being packaged so as to prevent access by fruit flies and other injurious insect pests. The package containing the papayas does not... trap per hectare and were checked for fruit flies at least once weekly by plant health officials of...

  8. Molecular and Clinical Effects of Green Tea and Fermented Papaya Preparation on Diabetes and Cardiovascular Diseases

    ClinicalTrials.gov

    2014-11-14

    Assess the Effect of Green Tea on Diabetes; Assess the Effect of Fermented Papaya Pretration on Diabetes; Effects of Green Tea and FPP on C-reactive Proteins; Effects of Green Tea and FPP of Lipid Profiles in Diabetes; Effect of Green Tea and FPP on Atheroma Formation

  9. Efforts to deregulate Rainbow papaya in Japan: Molecular Characterization of Transgene and Vector Inserts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Transformation plasmid-derived insert number and insert site sequence in 55-1 line papaya derivatives Rainbow and SunUp was determined as part of a larger petition to allow its import into Japan (Suzuki, et al., 2007, 2008). Three insertions were detected by Southern analysis and their correspondin...

  10. Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria.

    PubMed

    Shen, W; Yang, G; Chen, Y; Yan, P; Tuo, D; Li, X; Zhou, P

    2014-01-01

    RNA-mediated virus resistance based on natural antiviral RNA silencing has been exploited as a powerful tool for engineering virus resistance in plants. In this study, a conserved 3'-region (positions 9839-10117, 279 nt) of the capsid protein (CP) gene of papaya ringspot virus (PRSV), designated CP279, was used to generate an intron-containing hairpin RNA (ihpRNA) construct by one-step, zero-background ligation-independent cloning (OZ-LIC). The RNaseIII-deficient Escherichia coli strain M-JM109lacY was identified as the best choice for producing large quantities of specific ihpRNA-CP279. Resistance analyses and ELISA data verified that most papaya plants mechanically co-inoculated with TRIzol-extracted ihpRNA-CP279 and PRSV were resistant to PRSV, and resistance was maintained throughout the test period (>2 months post-inoculation). In contrast, a 1-2 day interval between sequential inoculation of PRSV and ihpRNA-CP279 did not result in complete protection against PRSV infection, but delayed the appearance of viral symptoms by 3 to 4 days. These findings indicate that direct mechanical inoculation of papaya plants with bacterially-expressed ihpRNA-CP279 targeting the PRSV CP gene can interfere with virus infection. This work lays a foundation for developing a non-transgenic approach to control PRSV by directly spraying plants with ihpRNA or crude bacterial extract preparations. PMID:25283861

  11. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ..., 2011 (76 FR 13972, Docket No. APHIS-2011- 0013), in which we announced the availability, for review and... weeds via the importation of fresh papaya fruit from Malaysia. DATES: Effective Date: August 11,...

  12. 76 FR 49725 - Notice of Decision To Authorize the Importation of Fresh Papaya Fruit From Malaysia into the...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-11

    ...designated phytosanitary measures will be sufficient to mitigate the risks of introducing or disseminating plant pests or noxious weeds via the importation of fresh papaya fruit from Malaysia. DATES: Effective Date: August 11, 2011. FOR FURTHER...

  13. 77 FR 31294 - Notice of Request for Extension of Approval of an Information Collection; Importation of Papaya...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-25

    ...papaya from Colombia and Ecuador into the continental United States. DATES: We will consider...importation, entry, or interstate movement of plants, plant products, and other...certain conditions before entering the continental United States to prevent the...

  14. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya...aqueous solution of latex. The resulting enzyme preparation may be used in a liquid...

  15. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya...aqueous solution of latex. The resulting enzyme preparation may be used in a liquid...

  16. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya...aqueous solution of latex. The resulting enzyme preparation may be used in a liquid...

  17. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya...aqueous solution of latex. The resulting enzyme preparation may be used in a liquid...

  18. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya...aqueous solution of latex. The resulting enzyme preparation may be used in a liquid...

  19. Antioxidant activity of bovine casein hydrolysates produced by Ficus carica L.-derived proteinase.

    PubMed

    Di Pierro, Giovanna; O'Keeffe, Martina B; Poyarkov, Alexey; Lomolino, Giovanna; FitzGerald, Richard J

    2014-08-01

    A Ficus carica L. latex proteinase preparation was investigated for its ability to produce antioxidant hydrolysates/peptides from bovine casein (CN). The Oxygen Radical Absorbance Capacity (ORAC) values for NaCN and ?-CN hydrolysates ranged from 0.06 to 0.18, and from 0.51 to 1.19?mol Trolox equivalents/mg freeze-dried sample, respectively. Gel permeation HPLC showed that the ?-CN hydrolysate with a degree of hydrolysis of 21% had 65% of peptide material with a molecular mass <500Da. The RP-UPLC profiles also indicated that ?-CN was substantially hydrolysed during the early stages of hydrolysis. Analysis of the 4h ?-CN hydrolysate by LC-ESI-MS/MS allowed identification of 8 peptide sequences with potential antioxidant properties. PMID:24629973

  20. Crystallization and preliminary X-ray analysis of four cysteine proteases from Ficus carica latex.

    PubMed

    Haesaerts, Sarah; Rodriguez Buitrago, John Alexander; Loris, Remy; Baeyens-Volant, Danielle; Azarkan, Mohamed

    2015-04-01

    The latex of the common fig (Ficus carica) contains a mixture of at least five cysteine proteases commonly known as ficins (EC 3.4.22.3). Four of these proteases were purified to homogeneity and crystals were obtained in a variety of conditions. The four ficin (iso)forms appear in ten different crystal forms. All diffracted to better than 2.10?Å resolution and for each form at least one crystal form diffracted to 1.60?Å resolution or higher. Ficin (iso)forms B and C share a common crystal form, suggesting close sequence and structural similarity. The latter diffracted to a resolution of 1.20?Å and belonged to space group P3?21 or P3?21, with unit-cell parameters a = b = 88.9, c = 55.9?Å. PMID:25849510

  1. ISOLATION AND PUNRIFICATION OF PSORALEN AND BERGAPTEN FROM FICUS CARICA L LEAVES BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

    PubMed Central

    Chunyan, Chi; Bo, Shi; Ping, Liang; Jingmei, Li; Ito, Yoichiro

    2009-01-01

    High-speed countercurrent chromatography was successfully applied for the first time for the separation of psoralen and bergapten from Ficus carica L leaves. The crudeextract obtained by light petroleum (b.p.: 60°C-90°C) from the dried leaves of Ficus carica L. was separated with a two-phase solvent system of n-Hexane-ethyl acetate-methanol-water (1:1:1:1, v/v). Each peak fraction was analyzed by high-performance liquid chromatography. The method yielded 4.4 mg of psoralen at 99.1% purity and 2.1 mg of bergapten at 98.2% purity from 400 mg of the crude extract in a single run. The two compounds were identified by 1H-NMR, 13C-NMR and MS. PMID:20351802

  2. Audubon Tree Study Program.

    ERIC Educational Resources Information Center

    National Audubon Society, New York, NY.

    Included are an illustrated student reader, "The Story of Trees," a leaders' guide, and a large tree chart with 37 colored pictures. The student reader reviews several aspects of trees: a definition of a tree; where and how trees grow; flowers, pollination and seed production; how trees make their food; how to recognize trees; seasonal changes;…

  3. Fungal Presence in Selected Tree Nuts and Dried Fruits.

    PubMed

    Tournas, V H; Niazi, N S; Kohn, J S

    2015-01-01

    Sixty-four tree nut samples (almonds, pecans, pine nuts, and walnuts) and 50 dried fruit samples (apricots, cranberries, papaya, pineapple, and raisins) were purchased from local supermarkets and analyzed for fungal contamination using conventional culture as well as molecular methods. The results of our study showed that the highest yeast and mold (YM) counts (5.34 log10 CFU g(-1)) were found in walnuts and the lowest in pecans. The most common mold in nuts was Aspergillus niger, relatively low numbers of A. flavus were found across the board, while Penicillium spp. were very common in pine nuts and walnuts. Low levels (2.00-2.84 log10 CFU g(-1)) of yeasts were recovered from only two pine nut samples. Fungal contamination in dried fruits was minimal (ranging from <2.00 to 3.86 log10 CFU g(-1)). The highest fungal levels were present in raisins. All papaya samples and the majority of cranberry, pineapple, and apricot samples were free of live fungi. The most common mold in dried fruits was A. niger followed by Penicillium spp. One apricot sample also contained low levels (2.00 log10 CFU g(-1)) of yeasts. PMID:26056470

  4. Fungal Presence in Selected Tree Nuts and Dried Fruits

    PubMed Central

    Tournas, VH; Niazi, NS; Kohn, JS

    2015-01-01

    Sixty-four tree nut samples (almonds, pecans, pine nuts, and walnuts) and 50 dried fruit samples (apricots, cranberries, papaya, pineapple, and raisins) were purchased from local supermarkets and analyzed for fungal contamination using conventional culture as well as molecular methods. The results of our study showed that the highest yeast and mold (YM) counts (5.34 log10 CFU g?1) were found in walnuts and the lowest in pecans. The most common mold in nuts was Aspergillus niger, relatively low numbers of A. flavus were found across the board, while Penicillium spp. were very common in pine nuts and walnuts. Low levels (2.00–2.84 log10 CFU g?1) of yeasts were recovered from only two pine nut samples. Fungal contamination in dried fruits was minimal (ranging from <2.00 to 3.86 log10 CFU g?1). The highest fungal levels were present in raisins. All papaya samples and the majority of cranberry, pineapple, and apricot samples were free of live fungi. The most common mold in dried fruits was A. niger followed by Penicillium spp. One apricot sample also contained low levels (2.00 log10 CFU g?1) of yeasts. PMID:26056470

  5. Accumulation and long-term decline of radiocaesium contamination in tropical fruit trees

    NASA Astrophysics Data System (ADS)

    Anjos, R. M.; Mosquera, B.; Carvalho, C.; Sanches, N.; Bastos, J.; Gomes, P. R. S.; Macario, K.

    2007-09-01

    The accumulation of 137Cs, 40K and NH 4+ in several organs of tropical plants species were studied through measurements of its concentrations from mango, avocado, guava, papaya, banana and chili pepper trees. Our goal was to infer their differences in the uptake and translocation of such ions to the aboveground plant parts and to establish the suitability of using radiocaesium as a tracer for the plant uptake of nutrients. The results indicate Cs + is better tracer for K + as it is for NH 4+.

  6. Minimizing Tree Automata for Unranked Trees

    E-print Network

    Martens, Wim

    Minimizing Tree Automata for Unranked Trees Wim Martens Joachim Niehren Minimizing Tree Automata for Unranked Trees ­ p.1/20 #12;What and Why? To study the minimization problem for deterministic automata over unranked trees. Bottom-up deterministic: theoretical interest. E.g. do results from deterministic automata

  7. Genetic characterization of fig tree mutants with molecular markers.

    PubMed

    Rodrigues, M G F; Martins, A B G; Desidério, J A; Bertoni, B W; Alves, M C

    2012-01-01

    The fig (Ficus carica L.) is a fruit tree of great world importance and, therefore, the genetic improvement becomes an important field of research for better crops, being necessary to gather information on this species, mainly regarding its genetic variability so that appropriate propagation projects and management are made. The improvement programs of fig trees using conventional procedures in order to obtain new cultivars are rare in many countries, such as Brazil, especially due to the little genetic variability and to the difficulties in obtaining plants from gamete fusion once the wasp Blastophaga psenes, responsible for the natural pollinating, is not found in Brazil. In this way, the mutagenic genetic improvement becomes a solution of it. For this reason, in an experiment conducted earlier, fig plants formed by cuttings treated with gamma ray were selected based on their agronomic characteristics of interest. We determined the genetic variability in these fig tree selections, using RAPD and AFLP molecular markers, comparing them to each other and to the Roxo-de-Valinhos, used as the standard. For the reactions of DNA amplification, 140 RAPD primers and 12 primer combinations for AFLP analysis were used. The selections did not differ genetically between themselves and between them and the Roxo-de-Valinhos cultivar. Techniques that can detect polymorphism between treatments, such as DNA sequencing, must be tested. The phenotypic variation of plants may be due to epigenetic variation, necessitating the use of techniques with methylation-sensitive restriction enzymes. PMID:22911583

  8. Asymmetric purine-pyrimidine distribution in cellular small RNA population of papaya

    PubMed Central

    2012-01-01

    Background The small RNAs (sRNA) are a regulatory class of RNA mainly represented by the 21 and 24-nucleotide size classes. The cellular sRNAs are processed by RNase III family enzyme dicer (Dicer like in plant) from a self-complementary hairpin loop or other type of RNA duplexes. The papaya genome has been sequenced, but its microRNAs and other regulatory RNAs are yet to be analyzed. Results We analyzed the genomic features of the papaya sRNA population from three sRNA deep sequencing libraries made from leaves, flowers, and leaves infected with Papaya Ringspot Virus (PRSV). We also used the deep sequencing data to annotate the micro RNA (miRNA) in papaya. We identified 60 miRNAs, 24 of which were conserved in other species, and 36 of which were novel miRNAs specific to papaya. In contrast to the Chargaff’s purine-pyrimidine equilibrium, cellular sRNA was significantly biased towards a purine rich population. Of the two purine bases, higher frequency of adenine was present in 23nt or longer sRNAs, while 22nt or shorter sRNAs were over represented by guanine bases. However, this bias was not observed in the annotated miRNAs in plants. The 21nt species were expressed from fewer loci but expressed at higher levels relative to the 24nt species. The highly expressed 21nt species were clustered in a few isolated locations of the genome. The PRSV infected leaves showed higher accumulation of 21 and 22nt sRNA compared to uninfected leaves. We observed higher accumulation of miRNA* of seven annotated miRNAs in virus-infected tissue, indicating the potential function of miRNA* under stressed conditions. Conclusions We have identified 60 miRNAs in papaya. Our study revealed the asymmetric purine-pyrimidine distribution in cellular sRNA population. The 21nt species of sRNAs have higher expression levels than 24nt sRNA. The miRNA* of some miRNAs shows higher accumulation in PRSV infected tissues, suggesting that these strands are not totally functionally redundant. The findings open a new avenue for further investigation of the sRNA silencing pathway in plants. PMID:23216749

  9. Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses

    PubMed Central

    Tuo, Decai; Shen, Wentao; Yang, Yong; Yan, Pu; Li, Xiaoying; Zhou, Peng

    2014-01-01

    Papaya ringspot virus (PRSV), Papaya leaf distortion mosaic virus (PLDMV), and Papaya mosaic virus (PapMV) produce similar symptoms in papaya. Each threatens commercial production of papaya on Hainan Island, China. In this study, a multiplex reverse transcription PCR assay was developed to detect simultaneously these three viruses by screening combinations of mixed primer pairs and optimizing the multiplex RT-PCR reaction conditions. A mixture of three specific primer pairs was used to amplify three distinct fragments of 613 bp from the P3 gene of PRSV, 355 bp from the CP gene of PLDMV, and 205 bp from the CP gene of PapMV, demonstrating the assay’s specificity. The sensitivity of the multiplex RT-PCR was evaluated by showing plasmids containing each of the viral target genes with 1.44 × 103, 1.79 × 103, and 1.91 × 102 copies for the three viruses could be detected successfully. The multiplex RT-PCR was applied successfully for detection of three viruses from 341 field samples collected from 18 counties of Hainan Island, China. Rates of single infections were 186/341 (54.5%), 93/341 (27.3%), and 3/341 (0.9%), for PRSV, PLDMV, and PapMV, respectively; 59/341 (17.3%) of the samples were co-infected with PRSV and PLDMV, which is the first time being reported in Hainan Island. This multiplex RT-PCR assay is a simple, rapid, sensitive, and cost-effective method for detecting multiple viruses in papaya and can be used for routine molecular diagnosis and epidemiological studies in papaya. PMID:25337891

  10. Technical Tree Climbing.

    ERIC Educational Resources Information Center

    Jenkins, Peter

    Tree climbing offers a safe, inexpensive adventure sport that can be performed almost anywhere. Using standard procedures practiced in tree surgery or rock climbing, almost any tree can be climbed. Tree climbing provides challenge and adventure as well as a vigorous upper-body workout. Tree Climbers International classifies trees using a system…

  11. Supercritical fluid chromatography with photodiode array detection for pesticide analysis in papaya and avocado samples.

    PubMed

    Pano-Farias, Norma S; Ceballos-Magaña, Silvia G; Gonzalez, Jorge; Jurado, José M; Muñiz-Valencia, Roberto

    2015-04-01

    To improve the analysis of pesticides in complex food matrices with economic importance, alternative chromatographic techniques, such as supercritical fluid chromatography, can be used. Supercritical fluid chromatography has barely been applied for pesticide analysis in food matrices. In this paper, an analytical method using supercritical fluid chromatography coupled to a photodiode array detection has been established for the first time for the quantification of pesticides in papaya and avocado. The extraction of methyl parathion, atrazine, ametryn, carbofuran, and carbaryl was performed through the quick, easy, cheap, effective, rugged, and safe methodology. The method was validated using papaya and avocado samples. For papaya, the correlation coefficient values were higher than 0.99; limits of detection and quantification ranged from 130-380 and 220-640 ?g/kg, respectively; recovery values ranged from 72.8-94.6%; precision was lower than 3%. For avocado, limit of detection values were ?450 ?g/kg; precision was lower than 11%; recoveries ranged from 50.0-94.2%. Method feasibility was tested for lime, banana, mango, and melon samples. Our results demonstrate that the proposed method is applicable to methyl parathion, atrazine, ametryn, and carbaryl, toxics pesticides used worldwide. The methodology presented in this work could be applicable to other fruits. PMID:25641906

  12. Fatty acids, coumarins and polyphenolic compounds of Ficus carica L. cv. Dottato: variation of bioactive compounds and biological activity of aerial parts.

    PubMed

    Marrelli, Mariangela; Statti, Giancarlo A; Tundis, Rosa; Menichini, Francesco; Conforti, Filomena

    2014-01-01

    Leaves, bark and woody part of Ficus carica L. cultivar Dottato collected in different months were examined to assess their chemical composition, antioxidant activity and phototoxicity on C32 human melanoma cells after UVA irradiation. The phytochemical investigation revealed different composition in the coumarin, fatty acid, polyphenol and flavonoid content. The second harvest of leaves and the first harvest of the bark possessed the highest antiradical activity with IC50 values of 64.00 ± 0.59 and 67.00 ± 1.09 ?g/mL, respectively. Harvest III of leaves showed the best inhibition of lipid peroxidation (IC50 = 1.48 ± 0.04 ?g/mL). Leaf samples of F. carica showed also the best antiproliferative activity in comparison with bark and woody part of F. carica. PMID:24087937

  13. Foraging on and consumption of two species of papaya pest mites, Tetranychus kanzawai and Panonychus citri (Acari: tetranychidae) by Mallada basalis (Neuroptera: Chrysopidae)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tetranychus kanzawai Kishida and Panonychus citri (McGregor) are two major acarine pests of the principal papaya variety in Taiwan, and they often co-occur in the same papaya screenhouses. This study measured prey acceptability, foraging schedule, short-term consumption rate, and handling time of la...

  14. Ephedra alte (joint pine): an invasive, problematic weedy species in forestry and fruit tree orchards in Jordan.

    PubMed

    Qasem, Jamal R

    2012-01-01

    A field survey was carried out to record plant species climbed by Ephedra alte in certain parts of Jordan during 2008-2010. Forty species of shrubs, ornamental, fruit, and forest trees belonging to 24 plant families suffered from the climbing habit of E. alte. Growth of host plants was adversely affected by E. alte growth that extended over their vegetation. In addition to its possible competition for water and nutrients, the extensive growth it forms over host species prevents photosynthesis, smothers growth and makes plants die underneath the extensive cover. However, E. alte did not climb all plant species, indicating a host preference range. Damaged fruit trees included Amygdalus communis, Citrus aurantifolia, Ficus carica, Olea europaea, Opuntia ficus-indica, and Punica granatum. Forestry species that were adversely affected included Acacia cyanophylla, Ceratonia siliqua, Crataegus azarolus, Cupressus sempervirens, Pinus halepensis, Pistacia atlantica, Pistacia palaestina, Quercus coccifera, Quercus infectoria, Retama raetam, Rhamnus palaestina, Rhus tripartita, and Zizyphus spina-christi. Woody ornamentals attacked were Ailanthus altissima, Hedera helix, Jasminum fruticans, Jasminum grandiflorum, Nerium oleander, and Pyracantha coccinea. Results indicated that E. alte is a strong competitive for light and can completely smother plants supporting its growth. A. communis, F. carica, R. palaestina, and C. azarolus were most frequently attacked. PMID:22645486

  15. Isolation and characterization of ethylene response factor family genes during development, ethylene regulation and stress treatments in papaya fruit.

    PubMed

    Li, Xueping; Zhu, Xiaoyang; Mao, Jia; Zou, Yuan; Fu, Danwen; Chen, Weixin; Lu, Wangjin

    2013-09-01

    Ethylene response factors (ERFs) play important roles in fruit development, ripening, defense responses and stress signaling pathways. After harvest, climacteric fruit such as papaya are subject to a range of problems associated with postharvest handling and storage treatments. There have been few attempts to evaluate the role of ERFs in fruit's responses to environmental stimuli. To investigate the transcriptional mechanisms underlying fruit developmental, ripening and stresses, we cloned four ERFs from papaya. The deduced amino acid sequence of CpERFs contained the conserved apetalous (AP2)/ERF domain, which shared high similarity with other reported AP2/ERF domains. The phylogeny, gene structures, and putatively conserved motifs in papaya ERF proteins were analyzed, and compared with those of Arabidopsis. Expression patterns of CpERFs were examined during fruit development, under 1-MCP treatment, ethephon treatment, biotic stress (temperature stress) and pathogen stress. CpERFs displayed differential expression patterns and expression levels under different experimental conditions. CpERF2 and CpERF3 showed a close association with fruit ripening and CpERFs had a high expression level in the earlier stages during the fruit development period. The expression of CpERFs strongly associated with stress response. These results support the role for papaya ERFs in transcriptional regulation of ripening-related or stress-respond genes and thus, in the regulation of papaya fruit-ripening processes and stress responses. PMID:23770597

  16. Meet the Ginkgo Tree Ginkgo Tree

    E-print Network

    Ashline, George

    Meet the Ginkgo Tree Ginkgo Tree Seedling Activity Tree location Try this... The Ginkgo tree has a unique leaf shape. To explore the shape of the Ginkgo leaf let's make a mini fan! · Look all around take the fan with you to keep cool all day long. You can also take your fan-shaped Ginkgo leaf

  17. Multiscale hierarchical assembly strategy and mechanical prowess in conch shells (Busycon carica).

    PubMed

    Li, Haoze; Xu, Zhi-Hui; Li, Xiaodong

    2013-12-01

    Seashells are natural body armors with superior mechanical strength and ultra-high toughness compared with their major constituent counterparts. What building blocks and architecture render seashells such mechanical prowess? In this study, micro/nanoscale structural and mechanical characterization of conch shells (Busycon carica) has been carried out. Here we show direct evidence that the previously claimed single-crystal third-order lamellae--the basic building blocks in conch shells are essentially assembled with aragonite nanoparticles of the size ranging from 20 to 45 nm. The nanoparticle-constructed third-order lamellae are not brittle, but ductile. The three-order crossed-lamellar architecture interlocks cracks via crack deflection along the interfaces in a three-dimensional manner, thus confining the damage in a small region. The findings advance the understanding of the mystery of conch shell's mechanical robustness, provide additional design guidelines for developing bioinspired nanomaterials, and lay a constitutive foundation for modeling the deformation behavior of seashells. PMID:24184467

  18. Ficus carica latex-mediated synthesis of silver nanoparticles and its application as a chemophotoprotective agent.

    PubMed

    Borase, Hemant P; Patil, Chandrashekhar D; Suryawanshi, Rahul K; Patil, Satish V

    2013-10-01

    The present work provides scientific support on the use of latex of Ficus carica to synthesize stable silver nanoparticles (AgNPs). AgNPs synthesized immediately after the addition of latex to silver nitrate solution at room temperature. Synthesized nanoparticles were of spherical shape with average size of 163.7 nm. Fourier transform infrared spectroscopy analysis revealed capping of proteins and phenolic compound on AgNPs, while X-ray diffraction analysis confirmed the fcc nature of AgNPs. Particles formed were stable for a long time (6 months). It was found that incorporation of AgNPs with 2 and 4% concentration exhibits synergistic increase in sun protection factor of commercial sunscreen and natural extracts ranging from 01 to 12,175% than control. Further characterization of latex and AgNPs revealed total phenolic content of 98.75 and 94.88 ?g/ml. The ferric ion reduction potentials of latex and AgNPs were 79.69 and 18.79%. Reduction potential of ascorbic acid was synergistically increased after cumulative preparation of ascorbic acid with latex and AgNPs and found to be 106.76 and 101.50% for ascorbic acid + latex and ascorbic acid + AgNPs, respectively. PMID:23881781

  19. Color and antioxidant characteristics of some fresh fig (Ficus carica L.) genotypes from northeastern Turkey.

    PubMed

    Ercisli, Sezai; Tosun, Murat; Karlidag, Huseyin; Dzubur, Ahmed; Hadziabulic, Semina; Aliman, Yasmina

    2012-09-01

    Fruit skin color, total phenolics, total anthocyanins, soluble solids content, titratable acidity and total antioxidant capacity in fresh fruits of a number of local and well-known fig (Ficus carica L.) genotypes and cultivars grown in northeastern Turkey were determined. TEAC (Trolox equivalent antioxidant capacity) and FRAP (ferric reducing antioxidant power) assays were used to determine total antioxidant capacity. Fruit skin color of genotypes were found to be very diverse, i.e., light green, light purple, purple, dark purple and black. The content of total phenolics, soluble solids content (SSC) and titratable acidity greatly varied in the range from 24 to 237 mg of gallic acid equivalent per 100 g fresh weight, 18.60 to 26.30 % and 0.16 to 0.47 % in local genotypes and studied cultivars. In general, total antioxidant capacities determined by two methods expressed higher values in the local fig genotypes compared with the cultivars. The results suggested that genotype is the main factor that determines difference in the composition of bioactive compounds in figs and provide information on putative health benefits locally grown genotypes. PMID:22618081

  20. Age and growth of the knobbed whelk Busycon carica (Gmelin 1791) in South Carolina subtidal waters

    USGS Publications Warehouse

    Eversole, A.G.; Anderson, W.D.; Isely, J.J.

    2008-01-01

    Knobbed whelk, Busycon carica (Gmelin, 1791), age and growth were estimated using tagged and recaptured individuals (n = 396) from areas off South Carolina coastal islands. Recaptured whelks were at large an average of 298 d (4-2,640 d). Growth, an increase in shell length (SL), was evident in 24% of the recaptured whelks, whereas 29% of recaptured individuals were the same size as when released and 47% were smaller than the released size. Mean growth rate was <0.001 mm SL/d and 0.022 mm SL/d if decreases in SL were assumed to be zero. Smaller whelks (???90 mm SL) at large for over one year grew seven times faster than larger whelks. The von Bertalanffy growth model: SL1 = 159.5(1 - e-0.0765(t+0.4162)), was developed from the mark - recapture whelks exhibiting growth. Based on a South Carolina minimum legal size of 102 mm SL, whelks recruit into the fishery at 13 y of age. The longevity, large size at maturity and slow growth suggest the potential for over harvest of knobbed whelk. Future whelk management plans may wish to consider whether economically viable commercial harvest can be sustainable.

  1. Detection of platypus-type L/D-peptide isomerase activity in aqueous extracts of papaya fruit.

    PubMed

    Arakawa, Kensuke; Koh, Jennifer M S; Crossett, Ben; Torres, Allan M; Kuchel, Philip W

    2012-09-01

    Peptide isomerase catalyses the post-translational isomerisation of the L: - to the D: -form of an amino acid residue around the N/C-termini of substrate peptides. To date, some peptide isomerases have been found in a limited number of animal secretions and cells. We show here that papaya extracts have weak peptide isomerase activity. The activity was detected in each 30-100 kDa fraction of the flesh and the seed extracts of unripe and ripe papaya fruit. The definitive activity was confirmed in the ripe papaya extracts, but even then it was much less active than that of the other peptide isomerases previously reported. The activity was markedly inhibited by methanol, and partly so by amastatin and diethyl pyrocarbonate. This is the first report of peptide isomerase activity in a plant and suggests that perhaps every living organism may have some peptide isomerase activity. PMID:22648682

  2. A phylogenetic analysis of the genus Carica L. (Caricaceae) based on restriction fragment length variation in a cpDNA intergenic spacer region

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The phylogenetic relationships among twelve wild and cultivated species of Carica (Caricaceae) were analyzed using restriction fragment length variation in a 3.2-kb PCR amplified intergenic spacer region of the chloroplast DNA. A total of 138 fragments representing 137 restriction sites accounting f...

  3. Essential Kurepa Trees Versus Essential Jech Kunen Trees1

    E-print Network

    Jin, Renling

    Essential Kurepa Trees Versus Essential Jech Kunen Trees1 Renling Jin2 & Saharon Shelah3 Abstract By an !1 tree we mean a tree of cardinality !1 and height !1. An !1 tree is called a Kurepa tree if all its levels are countable and it has more than !1 branches. An !1 tree is called a Jech Kunen tree if it has

  4. Ficus carica latex prevents invasion through induction of let-7d expression in GBM cell lines.

    PubMed

    Tezcan, Gulcin; Tunca, Berrin; Bekar, Ahmet; Yalcin, Murat; Sahin, Saliha; Budak, Ferah; Cecener, Gulsah; Egeli, Unal; Demir, Cevdet; Guvenc, Gokcen; Yilmaz, Gozde; Erkan, Leman Gizem; Malyer, Hulusi; Taskapilioglu, Mevlut Ozgur; Evrensel, Turkkan; Bilir, Ayhan

    2015-03-01

    Glioblastoma multiforme (GBM) is one of the deadliest human malignancies. A cure for GBM remains elusive, and the overall survival time is less than 1 year. Thus, the development of more efficient therapeutic approaches for the treatment of these patients is required. Induction of tumor cell death by certain phytochemicals derived from medicinal herbs and dietary plants has become a new frontier for cancer therapy research. Although the cancer suppressive effect of Ficus carica (fig) latex (FCL) has been determined in a few cancer types, the effect of this latex on GBM tumors has not been investigated. Therefore, in the current study, the anti-proliferative activity of FCL and the effect of the FCL-temozolomide (TMZ) combination were tested in the T98G, U-138 MG, and U-87 MG GBM cell lines using the WST-1 assay. The mechanism of cell death was analyzed using Annexin-V/FITC and TUNEL assays, and the effect of FCL on invasion was tested using the chick chorioallantoic membrane assay. To determine the effect of FCL on GBM progression, the expression levels of 40 GBM associated miRNAs were analyzed in T98G cells using RT-qPCR. According to the obtained data, FCL causes cell death in GBM cells with different responses to TMZ, and this effect is synergistically increased in combination with TMZ. In addition, the current study is the first to demonstrate the effect of FCL on modulation of let-7d expression, which may be an important underlying mechanism of the anti-invasive effect of this extract. PMID:25212824

  5. Protective Role of Ficus carica Stem Extract against Hepatic Oxidative Damage Induced by Methanol in Male Wistar Rats.

    PubMed

    Saoudi, Mongi; El Feki, Abdelfattah

    2012-01-01

    The present study was aimed to investigate the antioxidant activity of Ficus carica stem extract (FE) in methanol-induced hepatotoxicity in male Wistar rats. The rats were divided into two batches: 16 control rats (C) drinking tap water and 16 treated rats drinking Ficus carica stem extract for six weeks. Then, each group was divided into two subgroups, and one of them was intraperitoneally injected (i.p.) daily methanol at a dose of 2.37?g/kg body weight i.p. for 30 days, for four weeks. The results showed that FE was found to contain large amounts of polyphenols and carotenoids. The treatment with methanol exhibited a significant increase of serum hepatic biochemical parameters (ALT, AST, ALP, and LDH) and hepatic lipid peroxidation. Hepatic antioxidant enzymes, namely, SOD, CAT, and GSH-Px, were significantly decreased in methanol-treated animals. FE treatment prior to methanol intoxication has significant role in protecting animals from methanol-induced hepatic oxidative damage. PMID:22203864

  6. Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation.

    PubMed

    Eteraf-Oskouei, Tahereh; Allahyari, Saeideh; Akbarzadeh-Atashkhosrow, Arezu; Delazar, Abbas; Pashaii, Mahdiyeh; Gan, Siew Hua; Najafi, Moslem

    2015-01-01

    The antiangiogenesis effect of Ficus carica leaves extract in an air pouch model of inflammation was investigated in rat. Inflammation was induced by injection of carrageenan into pouches. After antioxidant capacity and total phenolic content (TPC) investigations, the extract was administered at 5, 25, and 50?mg/pouch, and then the volume of exudates, the cell number, TNF?, PGE2, and VEGF levels were measured. Angiogenesis of granulation tissues was determined by measuring hemoglobin content. Based on the DPPH assay, the extract had significant antioxidant activity with TPC of 11.70?mg GAE/100?g dry sample. In addition, leukocyte accumulation and volume of exudate were significantly inhibited by the extract. Moreover, it significantly decreased the production of TNF?, PGE2, and VEGF, while angiogenesis was significantly inhibited by all administered doses. Interestingly, attenuation of angiogenesis and inflammatory parameters (except leukocyte accumulation) by the extract was similar to that shown by diclofenac. The extract has anti-inflammatory effects and ameliorated cell influx and exudation to the site of the inflammatory response which may be related to the local inhibition of TNF?, PGE2, and VEGF levels as similarly shown by diclofenac. The antiangiogenesis and anti-VEGF effects of Ficus carica may be correlated with its significant antioxidant potentials. PMID:25977699

  7. High-performance thin layer chromatographic quantification of bioactive psoralen and daidzein in leaves of Ficus carica L.

    PubMed

    Ali, B; Mujeeb, M; Aeri, V; Mir, S R; Ahmad, S; Siddique, N A; Faiyazuddin, M; Shakeel, F

    2011-10-01

    This study was undertaken to quantify psoralen and daidzein by high-performance thin layer chromatography (HPTLC). The methanolic extract of 10 mg mL(-1) concentration solution was prepared for HPTLC quantification of psoralen and daidzein. HPTLC aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F(254) were used as the stationary phase. The working standard solution of psoralen and daidzein was applied along with the test sample solution by means of Camag Linomat IV sample applicator. R (f) values of psoralen and daidzein were found to be 0.60 and 0.88, whilst as their percentage values in methanolic extract were found to be 3.02% and 5.64% (w/w), respectively. A simple quantitative estimation method of psoralen and daidzein by HPTLC is reported that can be used for the quality control of marketed preparations containing Ficus carica. However, further study is warranted to isolate and quantify active constituents present in the leaves of F. carica by sophisticated techniques. PMID:21714728

  8. Methanolic Extract of Ficus carica Linn. Leaves Exerts Antiangiogenesis Effects Based on the Rat Air Pouch Model of Inflammation

    PubMed Central

    Eteraf-Oskouei, Tahereh; Allahyari, Saeideh; Akbarzadeh-Atashkhosrow, Arezu; Delazar, Abbas; Pashaii, Mahdiyeh; Gan, Siew Hua; Najafi, Moslem

    2015-01-01

    The antiangiogenesis effect of Ficus carica leaves extract in an air pouch model of inflammation was investigated in rat. Inflammation was induced by injection of carrageenan into pouches. After antioxidant capacity and total phenolic content (TPC) investigations, the extract was administered at 5, 25, and 50?mg/pouch, and then the volume of exudates, the cell number, TNF?, PGE2, and VEGF levels were measured. Angiogenesis of granulation tissues was determined by measuring hemoglobin content. Based on the DPPH assay, the extract had significant antioxidant activity with TPC of 11.70?mg GAE/100?g dry sample. In addition, leukocyte accumulation and volume of exudate were significantly inhibited by the extract. Moreover, it significantly decreased the production of TNF?, PGE2, and VEGF, while angiogenesis was significantly inhibited by all administered doses. Interestingly, attenuation of angiogenesis and inflammatory parameters (except leukocyte accumulation) by the extract was similar to that shown by diclofenac. The extract has anti-inflammatory effects and ameliorated cell influx and exudation to the site of the inflammatory response which may be related to the local inhibition of TNF?, PGE2, and VEGF levels as similarly shown by diclofenac. The antiangiogenesis and anti-VEGF effects of Ficus carica may be correlated with its significant antioxidant potentials. PMID:25977699

  9. A tree-to-tree model for statistical machine translation

    E-print Network

    Cowan, Brooke A. (Brooke Alissa), 1972-

    2008-01-01

    In this thesis, we take a statistical tree-to-tree approach to solving the problem of machine translation (MT). In a statistical tree-to-tree approach, first the source-language input is parsed into a syntactic tree ...

  10. Effect of temperature on the development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae, and the Asian papaya fruit fly, Bactrocera papayae, reared on guava diet.

    PubMed

    Danjuma, Solomon; Thaochan, Narit; Permkam, Surakrai; Satasook, Chutamas

    2014-01-01

    Members of the Bactrocera dorsalis Hendel (Diptera: Tephritidae) complex constitute well-recognized destructive pests of fruits in peninsular Thailand. The development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae Drew & Hancock, and the Asian papaya fruit fly, Bactrocera papayae Drew & Hancock, were compared at six constant temperatures of 15, 20, 25, 27, 30, and 35°C, 70 ± 5% relative humidity, and a photoperiod of 12:12 (L:D). The objectives were to determine the effect of temperature on the developmental stages for optimizing rearing and to understand the geographical pattern of occurrence of these fruit fly species. A strong and positive linear relationship was observed between temperature and developmental rate of immature stages of B. carambolae. Similarly, a strong and positive linear relationship was observed between temperature and developmental rate of B. papayae. A temperature summation model was used to estimate the lower threshold temperature and the thermal constant. Bactrocera papayae was significantly faster in development and higher in survival and appeared to be better adapted to low temperatures than B. carambolae, as it exhibited the lowest threshold temperatures at all immature stages. The observed differences in response to various temperatures revealed to some extent the impact of temperature on these species' distribution in peninsular Thailand and other parts of the world. PMID:25368070

  11. Effect of Temperature on the Development and Survival of Immature Stages of the Carambola Fruit Fly, Bactrocera carambolae, and the Asian Papaya Fruit Fly, Bactrocera papayae, Reared On Guava Diet

    PubMed Central

    Danjuma, Solomon; Thaochan, Narit; Permkam, Surakrai; Satasook, Chutamas

    2014-01-01

    Members of the Bactrocera dorsalis Hendel (Diptera: Tephritidae) complex constitute wellrecognized destructive pests of fruits in peninsular Thailand. The development and survival of immature stages of the carambola fruit fly, Bactrocera carambolae Drew & Hancock, and the Asian papaya fruit fly, Bactrocera papayae Drew & Hancock, were compared at six constant temperatures of 15, 20, 25, 27, 30, and 35°C, 70 ± 5% relative humidity, and a photoperiod of 12:12 (L:D). The objectives were to determine the effect of temperature on the developmental stages for optimizing rearing and to understand the geographical pattern of occurrence of these fruit fly species. A strong and positive linear relationship was observed between temperature and developmental rate of immature stages of B. carambolae. Similarly, a strong and positive linear relationship was observed between temperature and developmental rate of B. papayae. A temperature summation model was used to estimate the lower threshold temperature and the thermal constant. Bactrocera papayae was significantly faster in development and higher in survival and appeared to be better adapted to low temperatures than B. carambolae, as it exhibited the lowest threshold temperatures at all immature stages. The observed differences in response to various temperatures revealed to some extent the impact of temperature on these species' distribution in peninsular Thailand and other parts of the world. PMID:25368070

  12. TREE TRAILS Tree measurement is fundamental to the practice of forestry. Foresters count trees and measure trees.

    E-print Network

    1 TREE TRAILS Tree measurement is fundamental to the practice of forestry. Foresters count trees and measure trees. With just a few basic measurements, we can assign values to trees and compare them to each other. Tree Trails Tree Measurement Goal: Students will measure trees and explain how measurement

  13. Effect of pre-harvest calcium chloride applications on fruit calcium level and post-harvest anthracnose disease of papaya

    E-print Network

    Biggs, Alan R.

    Effect of pre-harvest calcium chloride applications on fruit calcium level and post. Calcium chloride at 1%, 1.5% and 2.0% concentrations significantly decreased spore germination. Calcium October 2013 Keywords: Papaya Anthracnose Calcium Disease incidence Disease severity a b s t r a c

  14. Effects of chitosan-based coatings containing peppermint essential oil on the quality of post-harvest papaya fruit

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Edible coatings comprised of antimicrobial polymers based on chitosan are promising technologies to preserve post-harvest fruit quality. In this study, we investigated the potential utility of a coating made from chitosan modified by N-acylation with fatty acid to preserve post-harvest papaya qualit...

  15. Managing Oriental Fruit Fly, Bactrocera dorsalis (Diptera: Tephritidae), Using Spinosad-Based Protein Bait Sprays in Papaya Orchards in Hawaii

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficacy of GF-120 Fruit Fly Bait was evaluated as a control of female oriental fruit fly, Bactrocera dorsalis (Hendel) in papaya orchards in Hawaii. Two important components of this study were field sanitation and mass trapping using the male-specific lure methyl eugenol. Three different spray ...

  16. Limit Laws for Functions of Fringe trees for Binary Search Trees and Random Recursive Trees

    E-print Network

    Janson, Svante

    Limit Laws for Functions of Fringe trees for Binary Search Trees and Random Recursive Trees Cecilia for sums of functions of subtrees of (random) binary search trees and random recursive trees. The proofs that the number of fringe trees of size k = kn in the binary search tree or in the random recursive tree (of total

  17. The Needs of Trees

    ERIC Educational Resources Information Center

    Boyd, Amy E.; Cooper, Jim

    2004-01-01

    Tree rings can be used not only to look at plant growth, but also to make connections between plant growth and resource availability. In this lesson, students in 2nd-4th grades use role-play to become familiar with basic requirements of trees and how availability of those resources is related to tree ring sizes and tree growth. These concepts can…

  18. Categorizing Ideas about Trees: A Tree of Trees

    PubMed Central

    Fisler, Marie; Lecointre, Guillaume

    2013-01-01

    The aim of this study is to explore whether matrices and MP trees used to produce systematic categories of organisms could be useful to produce categories of ideas in history of science. We study the history of the use of trees in systematics to represent the diversity of life from 1766 to 1991. We apply to those ideas a method inspired from coding homologous parts of organisms. We discretize conceptual parts of ideas, writings and drawings about trees contained in 41 main writings; we detect shared parts among authors and code them into a 91-characters matrix and use a tree representation to show who shares what with whom. In other words, we propose a hierarchical representation of the shared ideas about trees among authors: this produces a “tree of trees.” Then, we categorize schools of tree-representations. Classical schools like “cladists” and “pheneticists” are recovered but others are not: “gradists” are separated into two blocks, one of them being called here “grade theoreticians.” We propose new interesting categories like the “buffonian school,” the “metaphoricians,” and those using “strictly genealogical classifications.” We consider that networks are not useful to represent shared ideas at the present step of the study. A cladogram is made for showing who is sharing what with whom, but also heterobathmy and homoplasy of characters. The present cladogram is not modelling processes of transmission of ideas about trees, and here it is mostly used to test for proximity of ideas of the same age and for categorization. PMID:23950877

  19. Allomonal and hepatotoxic effects following methyl eugenol consumption in Bactrocera papayae male against Gekko monarchus.

    PubMed

    Wee, S L; Tan, K H

    2001-05-01

    Methyl eugenol (ME), is converted into two major phenylpropanoids, 2-allyl-4,5-dimethoxyphenol and trans-coniferyl alcohol, following consumption by the male fruit fly Bactrocera papayae. Chemical analysis of wild male B. papayae rectal glands, where the compounds are sequestered, revealed the presence of ME metabolites in varying quantities. These phenylpropanoids are shown to be involved in the fruit fly defense both in no-choice and choice feeding tests against the Malayan spiny gecko, Gekko monarchus. After being acclimatized to feeding on fruit flies, geckos consumed significantly fewer ME-fed male flies than controls that consumed all the ME-deprived male flies offered throughout a two-week period. Diagnosis of dissected livers from geckos that consumed ME-fed male flies revealed various abnormalities. These included discoloration and hardening of liver tissue, whitening of the gallbladder, or presence of tumor-like growths in all geckos that consumed ME-fed male flies. Control geckos fed on ME-deprived male flies had healthy livers. When given an alternative prey, geckos preferred to eat untreated house flies, Musca domestica to avoid preying on ME-fed fruit flies. PMID:11471947

  20. Compositional and functional dynamics of dried papaya as affected by storage time and packaging material.

    PubMed

    Udomkun, Patchimaporn; Nagle, Marcus; Argyropoulos, Dimitrios; Mahayothee, Busarakorn; Latif, Sajid; Müller, Joachim

    2016-04-01

    Papaya has been identified as a valuable source of nutrients and antioxidants, which are beneficial for human health. To preserve the nutritional properties after drying, appropriate storage specifications should be considered. This study aimed to investigate the quality and stability of air-dried papaya in terms of quality dynamics and behavior of bio-active compounds during storage for up to 9months in two packaging materials: aluminum laminated polyethylene and polyamide/polyethylene. Samples with moisture content (MC) of 0.1328gg(-1) and water activity (aw) of 0.5 were stored at 30°C and relative humidity (RH) of 40-50%. The MC, aw, degree of browning (DB) and 5-hydroxymethylfurfural (HMF) content were found to notably increase as storage progressed. On the contrary, there was a significant decrease in antioxidant capacity (DPPH, FRAP and ABTS), total phenolic (TP) and ascorbic acid (AA) contents. Packaging in aluminum laminated polyethylene under ambient conditions was found to better preserve bio-active compounds and retard increases in MC, aw and DB, when compared to polyamide/polyethylene. PMID:26593545

  1. Big Tree Measurement and Scoring: Virginia Big Tree Program

    E-print Network

    Virginia Tech

    accurate for straight trees on flat ground. Tree Height 1 big tree point is awarded for each foot of tree://www.americanforests.org/bigtrees/ champion-tree-measuring-guidelines #12;Trunk Circumference A. B. C. D. A. For typical trees on flat ground Spread ¼ big tree point is awarded for each foot of crown spread #12;

  2. Removal of Cr(VI) onto Ficus carica biosorbent from water.

    PubMed

    Gupta, V K; Pathania, Deepak; Agarwal, Shilpi; Sharma, Shikha

    2013-04-01

    The utilization of sustainable and biodegradable lignocellulosic fiber to detoxify the noxious Cr(VI) from wastewater is considered a versatile approach to clean up a contaminated aquatic environment. The aim of the present research is to assess the proficiency and mechanism of biosorption on Ficus carica bast fiber via isotherm models (Langmuir, Freundlich, Temkin, Harkin's-Jura, and Dubinin-Radushkevich), kinetic models, and thermodynamic parameters. The biomass extracted from fig plant was characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy. To optimize the maximum removal efficiency, different parameters like effect of initial concentration, effect of temperature, pH, and contact time were studied by batch method. The equilibrium data were best represented by the Langmuir isotherm model, and the maximum adsorption capacity of Cr(VI) onto biosorbent was found to be 19.68 mg/g. The pseudo-second-order kinetic model adequately described the kinetic data. The calculated values of thermodynamic parameters such as enthalpy change (?H(0)), entropy change (?S(0)), and free energy change (?G(0)) were 21.55 kJ/mol, 76.24 J/mol K, and -1.55 kJ/mol, respectively, at 30 °C which accounted for spontaneous and endothermic processes. The study of adsorbent capacity for Cr(VI) removal in the presence of Na(+), Mg(2+), Ca(2+), SO 4 (2-) , HCO 3 (-) and Cl(-) illustrated that the removal of Cr(VI) increased in the presence of HCO(3-) ions; the presence of Na(+), SO 4 (2-) or Cl(-) showed no significant influence on Cr(VI) adsorption, while Ca(2+) and Mg(2+) ions led to an insignificant decrease in Cr(VI) adsorption. Further, the desorption studies illustrated that 31.10% of metal ions can be removed from an aqueous system, out of which 26.63% of metal ions can be recovered by desorption in first cycle and the adsorbent can be reused. The results of the scale-up study show that the ecofriendly detoxification of Cr(VI) from aqueous systems was technologically feasible. PMID:22983603

  3. COMPONENT User's Guide Random trees

    E-print Network

    Page, Roderic

    COMPONENT User's Guide Chapter 6 Random trees COMPONENT can generate a variety of trees at "random," and for small numbers of leaves it can generate all possible trees. These trees have several uses: n as user trees for phylogeny programs that lack the facility to examine all trees or sets of random trees (e

  4. Role of irradiation in the green synthesis of silver nanoparticles mediated by fig (Ficus carica) leaf extract.

    PubMed

    Ulug, Bulent; Haluk Turkdemir, M; Cicek, Ahmet; Mete, Ahmet

    2015-01-25

    Biosynthesis of silver nanoparticles in an aqueous mixture of fig (Ficus carica) leaf extract and AgNO3 solution exposed to a set of irradiances at different wavelengths are studied. Nanoparticle formation for irradiances between 6.5 mW/cm(2) and 13.3 mW/cm(2) in the 330-550 nm wavelength range is investigated and the results are compared to those of the nanoparticles synthesized in the dark and under direct sunlight. Ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy, along with particle size analysis and transmission electron microscopy are employed for the characterization of samples and extracts. Irradiance is found to have profound influence on the reduction rates. However, size and spherical shape of the nanoparticles are persistent, irrespective of irradiance and wavelength. Irradiance is discussed to influence the particle formation and aggregation rates through the formation of free radicals in the fig extract. PMID:25062061

  5. Role of irradiation in the green synthesis of silver nanoparticles mediated by fig (Ficus carica) leaf extract

    NASA Astrophysics Data System (ADS)

    Ulug, Bulent; Haluk Turkdemir, M.; Cicek, Ahmet; Mete, Ahmet

    2015-01-01

    Biosynthesis of silver nanoparticles in an aqueous mixture of fig (Ficus carica) leaf extract and AgNO3 solution exposed to a set of irradiances at different wavelengths are studied. Nanoparticle formation for irradiances between 6.5 mW/cm2 and 13.3 mW/cm2 in the 330-550 nm wavelength range is investigated and the results are compared to those of the nanoparticles synthesized in the dark and under direct sunlight. Ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy, along with particle size analysis and transmission electron microscopy are employed for the characterization of samples and extracts. Irradiance is found to have profound influence on the reduction rates. However, size and spherical shape of the nanoparticles are persistent, irrespective of irradiance and wavelength. Irradiance is discussed to influence the particle formation and aggregation rates through the formation of free radicals in the fig extract.

  6. Hepatoprotective Activity of the Ethanolic Extract of Ficus caricaLinn. LeavesinCarbon Tetrachloride-Induced Hepatotoxicityin Rats.

    PubMed

    Mujeeb, Mohd; Alam Khan, Shah; Aeri, Vidhu; Ali, Babar

    2011-01-01

    The ethanolic extract of Ficus carica leaves was screened for hepatoprotective and antioxidant activity in hepatotoxic Albino rats induced via carbon tetrachloride. The degree of protection was measured by estimating biochemical parameters such as serum glutamate Oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase ( SGPT),totalprotein (TP), totalalbumin (TA), alkaline phosphatase (ALKP) and the level of total serum bilirubin. The extract in addition reduced CCl4 induced lipid peroxidation in - vivo and in - vitro. The ethanolic extract (50 mg/kg, 100mg/kg,200mg/kg)exhibited significant hepatoprotection incarbontetra chloride in toxicated rats in a dose dependant manner. The hepatoprotective effects of the extract were comparable with the standard drug silymarin 10)mg/kg body weight, IP). PMID:24250358

  7. From Gene Trees to Species Trees II: Species Tree Inference by Minimizing Deep

    E-print Network

    Zhang, Louxin

    From Gene Trees to Species Trees II: Species Tree Inference by Minimizing Deep Coalescence Events Louxin Zhang Abstract--When gene copies are sampled from various species, the resulting gene tree might disagree with the containing species tree. The primary causes of gene tree and species tree discord include

  8. On Gracefully Labeling Trees

    E-print Network

    Dhananjay P. Mehendale

    2013-10-25

    A method to obtain all possible graceful spanning trees in a complete graph is proposed. An algorithm to generate all the labeled spanning trees in a complete graph is developed and modified to generate all graceful spanning trees. The count of all possible graceful graphs in a complete graph is obtained. An upper bound on the count of gracefully labeled trees in a complete graph is obtained. We settle Graceful Tree Conjecture in the affirmative in two ways: 1) We show that all trees can be gracefully labeled by assigning the lowest label 1 to the so called special vertices of trees, i.e. prependant vertices or pendant vertices adjacent to prependant vertices. 2) We establish the existence of graceful labeling for all trees by associating distinct lattice paths with trees and by showing the existence of a lattice path for a tree of each isomorphism type by showing how to construct a lattice path recursively by starting from the lattice path for its pendant vertex deleted subtree, which is assumed to exists by induction, and carrying out appropriate modification of this lattice path. 3) We propose a crisp algorithm to gracefully label tree of every isomorphism type with the help of novel representation for any tree in terms of juxtaposition of paths. Lastly, we discuss an algorithm to find arbitrarily degree constrained graceful spanning tree and propose some problems for further investigation.

  9. Let's Explore the Ginkgo Tree! Ginkgo tree

    E-print Network

    Ashline, George

    of the Ginkgo tree in fossils dating back to time of the dinosaurs. Today you can create your own leaf fossil for the correct measurements of water and plaster. Let the plaster sit for the appropriate time. · Gently place take the fossil home and tell the story of how the Ginkgo tree was discovered. Do you want to lean more

  10. Radiation preservation of foods of plant origin. III. Tropical fruits: bananas, mangoes, and papayas

    SciTech Connect

    Thomas, P.

    1986-01-01

    The current status of research on the use of ionizing radiation for shelf life improvement and disinfestation of fresh tropical fruits like bananas, mangoes, and papayas are reviewed. The aspects covered are influence of maturity and physiological state of the fruits on delayed ripening and tolerance to radiation; varietal responses; changes in chemical constituents, volatiles, respiration, and ethylene evolution; biochemical mechanisms of delayed ripening and browning of irradiated fruits; and organoleptic quality. The efficacy of the combination of hot water dip and radiation treatments for control of postharvest fungal diseases are considered. The immediate potential of radiation as a quarantine treatment, in place of the currently used chemical fumigants, for disinfestation of fruit flies and mango seed weevil are discussed. Future prospects for irradiation of tropical fruits are discussed in the light of experience gained from studies conducted in different countries.146 references.

  11. Evolution of tree nutrition.

    PubMed

    Raven, John A; Andrews, Mitchell

    2010-09-01

    Using a broad definition of trees, the evolutionary origins of trees in a nutritional context is considered using data from the fossil record and molecular phylogeny. Trees are first known from the Late Devonian about 380 million years ago, originated polyphyletically at the pteridophyte grade of organization; the earliest gymnosperms were trees, and trees are polyphyletic in the angiosperms. Nutrient transporters, assimilatory pathways, homoiohydry (cuticle, intercellular gas spaces, stomata, endohydric water transport systems including xylem and phloem-like tissue) and arbuscular mycorrhizas preceded the origin of trees. Nutritional innovations that began uniquely in trees were the seed habit and, certainly (but not necessarily uniquely) in trees, ectomycorrhizas, cyanobacterial, actinorhizal and rhizobial (Parasponia, some legumes) diazotrophic symbioses and cluster roots. PMID:20581011

  12. Graph homomorphisms between trees

    E-print Network

    Csikvari, Peter

    In this paper we study several problems concerning the number of homomorphisms of trees. We begin with an algorithm for the number of homomorphisms from a tree to any graph. By using this algorithm and some transformations ...

  13. Minimal locked trees

    E-print Network

    Ballinger, Brad

    Locked tree linkages have been known to exist in the plane since 1998, but it is still open whether they have a polynomial-time characterization. This paper examines the properties needed for planar trees to lock, with a ...

  14. Tree Classification Software

    NASA Technical Reports Server (NTRS)

    Buntine, Wray

    1993-01-01

    This paper introduces the IND Tree Package to prospective users. IND does supervised learning using classification trees. This learning task is a basic tool used in the development of diagnosis, monitoring and expert systems. The IND Tree Package was developed as part of a NASA project to semi-automate the development of data analysis and modelling algorithms using artificial intelligence techniques. The IND Tree Package integrates features from CART and C4 with newer Bayesian and minimum encoding methods for growing classification trees and graphs. The IND Tree Package also provides an experimental control suite on top. The newer features give improved probability estimates often required in diagnostic and screening tasks. The package comes with a manual, Unix 'man' entries, and a guide to tree methods and research. The IND Tree Package is implemented in C under Unix and was beta-tested at university and commercial research laboratories in the United States.

  15. Simplifying Decision Trees

    E-print Network

    Quinlan, J.R.

    1986-12-01

    Many systems have been developed for constructing decision trees from collections of examples. Although the decision trees generated by these methods are accurate and efficient, they often suffer the disadvantage of ...

  16. Tea Tree Oil

    MedlinePLUS

    ... Top Key References Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (tea tree) oil: a review of ... 2006;19(1):50–62. Carson CF, Riley TV. Safety, efficacy and provenance of tea tree (Melaleuca ...

  17. Chem-Is-Tree.

    ERIC Educational Resources Information Center

    Barry, Dana M.

    1997-01-01

    Provides details on the chemical composition of trees including a definition of wood. Also includes an activity on anthocyanins as well as a discussion of the resistance of wood to solvents and chemicals. Lists interesting products from trees. (DDR)

  18. Grizzly Giant Tree

    USGS Multimedia Gallery

    This giant sequoia is named the Grizzly Giant, the oldest sequoia tree in the Mariposa Grove of Big Trees, located within Yosemite National Park. It is believed to be between 1,900 and 2,400 years old. ...

  19. Fig latex (Ficus carica L. cultivar Dottato) in combination with UV irradiation decreases the viability of A375 melanoma cells in vitro.

    PubMed

    Menichini, Giulio; Alfano, Carmine; Provenzano, Eugenio; Marrelli, Mariangela; Statti, Giancarlo A; Somma, Francesco; Menichini, Francesco; Conforti, Filomena

    2012-10-01

    Melanoma and nonmelanoma skin cancers are among the most prevalent cancers in the human population. In the present work latex of Ficus carica cultivar Dottato from Italy collected from fruits and leaves was examined to assess its free radical-scavenging activity with 1,1-diphenyl-2 picrylhydrazyl (DPPH) and its phototoxicity on A375 human melanoma cells. The latex obtained from the fruits of Ficus carica cv. Dottato showed the best antiradical activity with an IC50 value of 0.05 mg/ml while the latex obtained from the leaves showed the best antiproliferative activity with an IC50 value of 1.5 ?g/ml on the human tumor cell line A375 (melanoma) after irradiation at a specific UVA dose (1.08 J/cm2). Control experiments with UVA light or drugs alone were carried out without significant cytotoxic effects. Polyphenolic content of the samples was also evaluated. This is the first study comparing F. carica latex of leaves and fruits. Plant derived natural products have long been and will continue to be an important source for anticancer drug development. PMID:22339064

  20. Evaluation of General Toxicity, Anti-Oxidant Activity and Effects of Ficus Carica Leaves Extract on Ischemia/Reperfusion Injuries in Isolated Heart of Rat

    PubMed Central

    Allahyari, Saeideh; Delazar, Abbas; Najafi, Moslem

    2014-01-01

    Purpose: This study was aimed to evaluate general toxicity, anti-oxidant activity and effects of Ficus carica leaves extract on ischemia/reperfusion injuries. Methods: Antioxidant activity, total phenolic and flavonoid compounds of 70% methanolic extract of Ficus carica leaves were measured. The general toxicity test was carried out by brine shrimp lethality assay. Isolated hearts of male rats were mounted on a Langendorff apparatus and perfused with modified Krebs-Henseleit solution. In control group, the hearts were perfused with normal Krebs solution, however, treatment groups received enriched solution with the extract (0.04, 0.2 and 1 mg/ml) during stabilization and reperfusion (after 30 min global ischemia), respectively. Cardiac arrhythmias were analyzed and TTC method was used for infarct size determination. Results: The extract displayed antioxidant activity in the DPPH assay (RC50=0.06666 mg/ml). Total phenolic content was 12.29 mg GAE/100 g dry sample and the amount of flavonoids was calculated 40.729 mg/g. LD50 value by brine shrimp test was 0.158 mg/ml. The extract decreased number of VEBs, incidence and duration of Rev VF with clear reduction in infarct size and infarct volume (P<0.001). Conclusion: Ficus carica decreased ischemia/reperfusion-induced injuries. These protections are probably due to antioxidant capacity and the existence of flavonoid and phenolic compounds in the extract. PMID:25671192

  1. Binary Search Trees Page 1 Binary Search Trees

    E-print Network

    Allan, Vicki H.

    Binary Search Trees Page 1 Chapter 4 Trees Binary Search Trees · Definition o All keys are distinct subtree is greater than the root. o Left and right subtrees are binary search trees. · Figure 1 is an example: Figure 1 Binary Search Tree · Where would you add X, B, and E? · At seats, build tree from

  2. Discordance of species trees with their most likely gene trees

    E-print Network

    Degnan, James

    Discordance of species trees with their most likely gene trees James H. Degnan & Noah A. Rosenberg sort during speciation, gene trees may differ in topology from each other and from species trees gene tree discordance is so common that the most likely gene tree topology to evolve along the branches

  3. Avery Andrews' Trees Preprocessor

    E-print Network

    Pratt, Vaughan

    Avery Andrews' Trees Preprocessor Consider the tree shown in (1): (1) S NP that book S/NP NP I VP/NP V want VP/NP to VP/NP V ask NP Mary VP/NP to VP/NP V send NP Tom NP/NP e Making trees like this in T E X or L A T E X can be a cause for dread. But it can become quite simple and fun with the trees

  4. Decision-Tree Program

    NASA Technical Reports Server (NTRS)

    Buntine, Wray

    1994-01-01

    IND computer program introduces Bayesian and Markov/maximum-likelihood (MML) methods and more-sophisticated methods of searching in growing trees. Produces more-accurate class-probability estimates important in applications like diagnosis. Provides range of features and styles with convenience for casual user, fine-tuning for advanced user or for those interested in research. Consists of four basic kinds of routines: data-manipulation, tree-generation, tree-testing, and tree-display. Written in C language.

  5. Basswood Tree Seedling activity

    E-print Network

    Ashline, George

    board or sturdy white paper · Glue · Markers · Ruler · Tell Me, Tree by Gail Gibbons Procedure: · Read the book Tell Me, Tree. · Collect a leaf that has no holes. · Cut a piece of poster board that is about 6. · On the first line, write the name of the tree. · On the second line, write the characteristics of the tree

  6. Pecan tree biomass estimates

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Allometric equations were developed for orchard-grown pecan [Carya illinoinensis (Wangenh.) C. Koch] trees. Trees, ranging in size from 22 to 33 cm in trunk diameter 1.4 m above the ground, were destructively harvested from two sites. The entire above-ground portion of the trees was harvested and...

  7. The Wish Tree Project

    ERIC Educational Resources Information Center

    Brooks, Sarah DeWitt

    2010-01-01

    This article describes the author's experience in implementing a Wish Tree project in her school in an effort to bring the school community together with a positive art-making experience during a potentially stressful time. The concept of a wish tree is simple: plant a tree; provide tags and pencils for writing wishes; and encourage everyone to…

  8. Minnesota's Forest Trees. Revised.

    ERIC Educational Resources Information Center

    Miles, William R.; Fuller, Bruce L.

    This bulletin describes 46 of the more common trees found in Minnesota's forests and windbreaks. The bulletin contains two tree keys, a summer key and a winter key, to help the reader identify these trees. Besides the two keys, the bulletin includes an introduction, instructions for key use, illustrations of leaf characteristics and twig…

  9. Structural Equation Model Trees

    ERIC Educational Resources Information Center

    Brandmaier, Andreas M.; von Oertzen, Timo; McArdle, John J.; Lindenberger, Ulman

    2013-01-01

    In the behavioral and social sciences, structural equation models (SEMs) have become widely accepted as a modeling tool for the relation between latent and observed variables. SEMs can be seen as a unification of several multivariate analysis techniques. SEM Trees combine the strengths of SEMs and the decision tree paradigm by building tree

  10. Winter Birch Trees

    ERIC Educational Resources Information Center

    Sweeney, Debra; Rounds, Judy

    2011-01-01

    Trees are great inspiration for artists. Many art teachers find themselves inspired and maybe somewhat obsessed with the natural beauty and elegance of the lofty tree, and how it changes through the seasons. One such tree that grows in several regions and always looks magnificent, regardless of the time of year, is the birch. In this article, the…

  11. Representing Trees with Constraints 

    E-print Network

    Curry, Benjamin; Wiggins, Geraint; Hayes, Gillian

    This paper presents a method for representing trees using constraint logic programming over finite domains. We describe a class of trees that is of particular interest to us and how we can represent the set of trees belonging to that class using...

  12. Effect of irradiation on the biochemical and organoleptic changes during the ripening of papaya and mango fruits

    NASA Astrophysics Data System (ADS)

    Lacroix, Monique; Bernard, Linda; Jobin, Michele; Milot, Sylvain; Gagnon, Marcel

    Papaya and mango rot caused by fungi is a major problem during storage and marketing. Gamma irradiation treatment was used to determine its effect on the quality of papayas and mangoes irradiated at 0,5 to 0,95 kGy. The level of respiration, soluble solids, texture, vitamin C and the sensorial evaluation were effectuated. The results indicate that irradiation treatment reduces significantly (p ? 0,001) the level of respiration and significantly (p ? 0,001) weakens the texture of mangoes. The content of soluble solids and vitamin C are not significantly affected by the irradiation. The sensory evaluation indicates that up to 0,95 kGy the sensorial quality is not changed.

  13. Staphylococcal enterotoxin A gene-carrying Staphylococcus aureus isolated from foods and its control by crude alkaloid from papaya leaves.

    PubMed

    Handayani, Lita; Faridah, Didah Nur; Kusumaningrum, Harsi D

    2014-11-01

    Staphylococcus aureus is a known pathogen causing intoxication by producing enterotoxins in food. Staphylococcal enterotoxin A is one of the enterotoxins commonly implicated in staphylococcal food poisoning. The ability of crude alkaloid extract from papaya leaves to inhibit the growth of S. aureus and staphylococcal enterotoxin A synthesis was investigated. Staphylococcal enterotoxin A gene-carrying S. aureus was isolated from raw milk and ready-to-eat foods. Crude alkaloid was extracted from ground, dried papaya leaves using ultrasonic-assisted extraction, and a MIC of the alkaloid was determined by the broth macrodilution method. Furthermore, S. aureus isolate was exposed to the crude alkaloid extract at one- and twofold MIC, and the expression of sea was subsequently analyzed using a quantitative reverse transcription real-time PCR. Ten isolates of S. aureus were obtained, and nine of those isolates were sea carriers. The yield of crude alkaloid extract was 0.48 to 1.82% per dry weight of papaya leaves. A MIC of crude alkaloid to S. aureus was 0.25 mg/ml. After exposure to the alkaloid at 0.25 and 0.5 mg/ml for 2 h, a significant increase in cycle threshold values of sea was observed. The sea was expressed 29 and 41 times less when S. aureus was exposed to crude alkaloid at one- and twofold MIC, respectively. This study revealed that crude alkaloid of papaya leaves could control staphylococcal enterotoxin A gene-carrying S. aureus by suppressing the expression of sea, in addition to the ability to inhibit the growth of S. aureus. The expression of sea was successfully quantified. PMID:25364936

  14. [Parasitism in Trialeurodes variabilis (Quaintance) (Hemiptera: Aleyrodidae) by Encarsia hispida De Santis (Hymenoptera: Aphelinidae), in papaya, in Brazil].

    PubMed

    Lourenção, André L; Fancelli, Marilene; Costa, Valmir A; Ribeiro, Nicolle C

    2007-01-01

    Infestation of Trialeurodes variabilis (Quaintance) was observed in October 2004, in papaya plants of cultivar Sunrise Solo, under screenhouse conditions, in Cruz das Almas, State of Bahia, Brazil. In infested leaves, around 20% of parasitism on nymphs was verified. Leaves with parasitized nymphs were kept in laboratory until emergence of the parasitoid, identified as Encarsia hispida De Santis. This is the first time that this parasitoid was detected on T. variabilis nymphs in Brazil. PMID:17420874

  15. Growth of a Pine Tree

    ERIC Educational Resources Information Center

    Rollinson, Susan Wells

    2012-01-01

    The growth of a pine tree is examined by preparing "tree cookies" (cross-sectional disks) between whorls of branches. The use of Christmas trees allows the tree cookies to be obtained with inexpensive, commonly available tools. Students use the tree cookies to investigate the annual growth of the tree and how it corresponds to the number of whorls…

  16. Distributed Contour Trees

    SciTech Connect

    Morozov, Dmitriy; Weber, Gunther H.

    2014-03-31

    Topological techniques provide robust tools for data analysis. They are used, for example, for feature extraction, for data de-noising, and for comparison of data sets. This chapter concerns contour trees, a topological descriptor that records the connectivity of the isosurfaces of scalar functions. These trees are fundamental to analysis and visualization of physical phenomena modeled by real-valued measurements. We study the parallel analysis of contour trees. After describing a particular representation of a contour tree, called local{global representation, we illustrate how di#11;erent problems that rely on contour trees can be solved in parallel with minimal communication.

  17. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Color-shifted complexes Weighted spanning tree enumerators of color-shifted complexes Ghodratollah Aalipour1,2 Art Duval1 1University Ghodratollah Aalipour, Art Duval Spanning tree enumerators of color-shifted complexes #12;Spanning trees

  18. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Complete colorful complexes Weighted spanning tree enumerators of complete colorful complexes Ghodratollah Aalipour1,2 Art Duval1 1 Aalipour, Art Duval Spanning tree enumerators of complete colorful complexes #12;Spanning trees of graphs

  19. Discordance of Species Trees with Their Most Likely Gene Trees

    E-print Network

    Rosenberg, Noah

    Discordance of Species Trees with Their Most Likely Gene Trees James H. Degnan1 , Noah A. Rosenberg way in which lineages sort during speciation, gene trees may differ in topology from each other for which gene tree discordance is so common that the most likely gene tree topology to evolve along

  20. Condensation in nongeneric trees

    E-print Network

    Thordur Jonsson; Sigurdur Orn Stefansson

    2011-01-04

    We study nongeneric planar trees and prove the existence of a Gibbs measure on infinite trees obtained as a weak limit of the finite volume measures. It is shown that in the infinite volume limit there arises exactly one vertex of infinite degree and the rest of the tree is distributed like a subcritical Galton-Watson tree with mean offspring probability $mtrees in the thermodynamic limit and show it goes like $(1-m)N$ where $N$ is the size of the tree. These trees have infinite spectral dimension with probability one but the spectral dimension calculated from the ensemble average of the generating function for return probabilities is given by $2\\beta -2$ if the weight $w_n$ of a vertex of degree $n$ is asymptotic to $n^{-\\beta}$.

  1. Effects of ozone exposure on 'Golden' papaya fruit by photoacoustic phase-resolved method: Physiological changes associated with carbon dioxide and ethylene emission rates during ripening

    SciTech Connect

    Correa, Savio Figueira; Brito Paiva, Luisa; Mota do Couto, Flavio; Gomes da Silva, Marcelo; Silva Sthel, Marcelo; Vargas, Helion; Mota, Leonardo; Goncalves de Oliveira, Jurandi; Miklos, Andras

    2011-06-01

    This work addresses the effects of ozone activity on the physiology of 'Golden' papaya fruit. Depth profile analysis of double-layer biological samples was accomplished using the phase-resolved photoacoustic spectroscopy. The feasibility of the method was demonstrated by singling out the spectra of the cuticle and the pigment layers of papaya fruit. The same approach was used to monitor changes occurring on the fruit during ripening when exposed to ozone. In addition, one has performed real time studies of fluorescence parameters and the emission rates of carbon dioxide and ethylene. Finally, the amount of pigments and the changes in waxy cuticle have been monitored. Results indicate that a fruit deliberately subjected to ozone at a level of 6 ppmv underwent ripening sooner (at least 24-48 h) than a fruit stored at ambient conditions. Moreover, ozone caused a reduction in the maximum quantum yield of photosynthetic apparatus located within the skin of papaya fruit.

  2. Species integrity in trees.

    PubMed

    Ortiz-Barrientos, Daniel; Baack, Eric J

    2014-09-01

    From California sequoia, to Australian eucalyptus, to the outstanding diversity of Amazonian forests, trees are fundamental to many processes in ecology and evolution. Trees define the communities that they inhabit, are host to a multiplicity of other organisms and can determine the ecological dynamics of other plants and animals. Trees are also at the heart of major patterns of biodiversity such as the latitudinal gradient of species diversity and thus are important systems for studying the origin of new plant species. Although the role of trees in community assembly and ecological succession is partially understood, the origin of tree diversity remains largely opaque. For instance, the relative importance of differing habitats and phenologies as barriers to hybridization between closely related species is still largely uncharacterized in trees. Consequently, we know very little about the origin of trees species and their integrity. Similarly, studies on the interplay between speciation and tree community assembly are in their infancy and so are studies on how processes like forest maturation modifies the context in which reproductive isolation evolves. In this issue of Molecular Ecology, Lindtke et al. (2014) and Lagache et al. (2014) overcome some traditional difficulties in studying mating systems and sexual isolation in the iconic oaks and poplars, providing novel insights about the integrity of tree species and on how ecology leads to variation in selection on reproductive isolation over time and space. PMID:25155715

  3. Texas Shade Trees 

    E-print Network

    Unknown

    2011-09-05

    second among the states in the number of bearing peach trees, having 9,737,000 trees. A majority of these trees were growing in a few districts; 10 per cent were located in a single county, and more than 50 per cent in half ·a dozen counties of Northeast... Texas. Of the 417,770 farms reported in the state in 1910, only 108,959, or 25 per cent, reported any peach trees. A lack of knowledge of peach culture is believed to be responsible ior this uneven distribution of orchards. To meet this situation...

  4. COMPONENT User's Guide Working with trees

    E-print Network

    Page, Roderic

    COMPONENT User's Guide Chapter 2 Working with trees This chapter gives you information on basic tree operations. The topics covered include: n reading and writing files of trees n editing trees n displaying and printing trees n selecting subsets of trees n pruning trees n rooting trees n computing tree

  5. COMPONENT User's Guide Comparing trees

    E-print Network

    Page, Roderic

    COMPONENT User's Guide Chapter 5 Comparing trees This chapter describes the tree comparison measures available in COMPONENT, and the various ways you can compare trees using the program. Among the possible uses of tree comparison measures are: n comparing trees for the same taxa computed from different

  6. Traditional agroecosystems as conservatories and incubators of cultivated plant varietal diversity: the case of fig (Ficus carica L.) in Morocco

    PubMed Central

    2010-01-01

    Background Traditional agroecosystems are known to host both large crop species diversity and high within crop genetic diversity. In a context of global change, this diversity may be needed to feed the world. Are these agroecosystems museums (i.e. large core collections) or cradles of diversity? We investigated this question for a clonally propagated plant, fig (Ficus carica), within its native range, in Morocco, but as far away as possible from supposed centers of domestication. Results Fig varieties were locally numerous. They were found to be mainly highly local and corresponded to clones propagated vegetatively. Nevertheless these clones were often sufficiently old to have accumulated somatic mutations for selected traits (fig skin color) and at neutral loci (microsatellite markers). Further the pattern of spatial genetic structure was similar to the pattern expected in natural population for a mutation/drift/migration model at equilibrium, with homogeneous levels of local genetic diversity throughout Moroccan traditional agroecosystems. Conclusions We conclude that traditional agroecosystems constitue active incubators of varietal diversity even for clonally propagated crop species, and even when varieties correspond to clones that are often old. As only female fig is cultivated, wild fig and cultivated fig probably constitute a single evolutionary unit within these traditional agroecosystems. Core collections, however useful, are museums and hence cannot serve the same functions as traditional agroecosystems. PMID:20167055

  7. Cytotoxic Effects of Different Extracts and Latex of Ficus carica L. on HeLa cell Line

    PubMed Central

    Khodarahmi, Ghadam Ali; Ghasemi, Nasrollah; Hassanzadeh, Farshid; Safaie, Marzieh

    2011-01-01

    It has been reported that latex and extracts of different species of Ficus are cytotoxic to some human cancerous cell lines. In this study, cytotoxicity of fruit and leaf extracts as well as the latex of Ficuscarica L. on HeLa cell line were evaluated. ethanolic extracts of leaves and fruits were prepared through percolation and ethyl acetate and dichloromethane extracts were prepared by reflux method. Cytotoxic effects of these extracts and latex against HeLa cell line were then examined. Briefly, He Lacells were seeded at 2 × 104 cells/mL in 96-well plates. After 24 h incubation at 37°C, the cells were treated with different concentrations of the extracts or latex. The viability of the cells was determined by the reduction of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) from formazan following 48 h incubation and the absorbance was measured at 540 nm using an ELISA plate reader. The results indicated that the latex and different extracts of Ficus carica could reduce the viability of the He Lacells at concentrations as low as 2 µg/mL in a dose dependent manner. The approximate IC50 values of the ethanolic, ethyl acetate and dichloromethane extracts of the leaves and fruits were 10, 19, 12 µg/mL and 12, 12, 11.5 µg/mL, respectively. The IC50 for the latex was about 17 µg/mL. PMID:24250354

  8. Identification, purification and characterization of a novel collagenolytic serine protease from fig (Ficus carica var. Brown Turkey) latex.

    PubMed

    Raskovic, Brankica; Bozovic, Olga; Prodanovic, Radivoje; Niketic, Vesna; Polovic, Natalija

    2014-12-01

    A novel collagenolytic serine protease was identified and then purified (along with ficin) to apparent homogeneity from the latex of fig (Ficus carica, var. Brown Turkey) by two step chromatographic procedure using gel and covalent chromatography. The enzyme is a monomeric protein of molecular mass of 41 ± 9 kDa as estimated by analytical gel filtration chromatography. It is an acidic protein with a pI value of approximately 5 and optimal activity at pH 8.0-8.5 and temperature 60°C. The enzymatic activity was strongly inhibited by PMSF and Pefabloc SC, indicating that the enzyme is a serine protease. The enzyme showed specificity towards gelatin and collagen (215 GDU/mg and 24.8 CDU/mg, respectively) and non-specific protease activity (0.18 U/mg against casein). The enzyme was stable and retained full activity over a broad range of pH and temperature. The fig latex collagenolytic protease is potentially useful as a non-microbial enzyme with collagenolytic activity for various applications in the fields of biochemistry, biotechnology and medicine. PMID:24982021

  9. Effects of Ficus carica paste on constipation induced by a high-protein feed and movement restriction in beagles.

    PubMed

    Oh, Hong-Geun; Lee, Hak-Yong; Seo, Min-Young; Kang, Young-Rye; Kim, Jung-Hoon; Park, Jung-Woo; Kim, Ok-Jin; Back, Hyang-Im; Kim, Sun-Young; Oh, Mi-Ra; Park, Soo-Hyun; Kim, Min-Gul; Jeon, Ji-Young; Hwang, Min-Ho; Shin, Sook-Jeong; Chae, Soo-Wan

    2011-12-01

    Constipation is one of the most common functional digestive complaints worldwide. We investigated the laxative effects of figs (Ficus carica L) in a beagle model of constipation induced by high protein diet and movement restriction. The experiments were consecutively conducted over 9 weeks divided into 3 periods of 3 weeks each. All 15 beagles were subjected to a non-treatment (control) period, a constipation induction period, and a fig paste treatment period. We administered fig paste (12 g/kg daily, by gavage) for 3 weeks following a 3-week period of constipation induction in dogs. Segmental colonic transit time (CTT) was measured by counting radiopaque markers (Kolomark) using a radiograph performed every 6 h after feeding Kolomark capsules, until capsules were no longer observed. Fig paste significantly increased fecal quantity in constipated dogs, and segmental CTT was also reduced following fig paste administration. There were no significant differences in feed intake, water intake, body weight, or blood test results, between the constipation and fig paste administration periods. Our results demonstrate that fig is an effective treatment for constipation in beagles. Specifically, stool weight increased and segmental CTT decreased. Fig pastes may be useful as a complementary medicine in humans suffering from chronic constipation. PMID:22232635

  10. Cytotoxic Effects of Different Extracts and Latex of Ficus carica L. on HeLa cell Line.

    PubMed

    Khodarahmi, Ghadam Ali; Ghasemi, Nasrollah; Hassanzadeh, Farshid; Safaie, Marzieh

    2011-01-01

    It has been reported that latex and extracts of different species of Ficus are cytotoxic to some human cancerous cell lines. In this study, cytotoxicity of fruit and leaf extracts as well as the latex of Ficuscarica L. on HeLa cell line were evaluated. ethanolic extracts of leaves and fruits were prepared through percolation and ethyl acetate and dichloromethane extracts were prepared by reflux method. Cytotoxic effects of these extracts and latex against HeLa cell line were then examined. Briefly, He Lacells were seeded at 2 × 10(4) cells/mL in 96-well plates. After 24 h incubation at 37(°)C, the cells were treated with different concentrations of the extracts or latex. The viability of the cells was determined by the reduction of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) from formazan following 48 h incubation and the absorbance was measured at 540 nm using an ELISA plate reader. The results indicated that the latex and different extracts of Ficus carica could reduce the viability of the He Lacells at concentrations as low as 2 µg/mL in a dose dependent manner. The approximate IC50 values of the ethanolic, ethyl acetate and dichloromethane extracts of the leaves and fruits were 10, 19, 12 µg/mL and 12, 12, 11.5 µg/mL, respectively. The IC50 for the latex was about 17 µg/mL. PMID:24250354

  11. Evidence for emergence of sex-determining gene(s) in a centromeric region in Vasconcellea parviflora.

    PubMed

    Iovene, Marina; Yu, Qingyi; Ming, Ray; Jiang, Jiming

    2015-02-01

    Sex chromosomes have been studied in many plant and animal species. However, few species are suitable as models to study the evolutionary histories of sex chromosomes. We previously demonstrated that papaya (Carica papaya) (2n = 2x = 18), a fruit tree in the family Caricaceae, contains recently emerged but cytologically heteromorphic X/Y chromosomes. We have been intrigued by the possible presence and evolution of sex chromosomes in other dioecious Caricaceae species. We selected a set of 22 bacterial artificial chromosome (BAC) clones that are distributed along the papaya X/Y chromosomes. These BACs were mapped to the meiotic pachytene chromosomes of Vasconcellea parviflora (2n = 2x = 18), a species that diverged from papaya ?27 million years ago. We demonstrate that V. parviflora contains a pair of heteromorphic X/Y chromosomes that are homologous to the papaya X/Y chromosomes. The comparative mapping results revealed that the male-specific regions of the Y chromosomes (MSYs) probably initiated near the centromere of the Y chromosomes in both species. The two MSYs, however, shared only a small chromosomal domain near the centromere in otherwise rearranged chromosomes. The V. parviflora MSY expanded toward the short arm of the chromosome, whereas the papaya MSY expanded in the opposite direction. Most BACs mapped to papaya MSY were not located in V. parviflora MSY, revealing different DNA compositions in the two MSYs. These results suggest that mutation of gene(s) in the centromeric region may have triggered sex chromosome evolution in these plant species. PMID:25480779

  12. Apple Tree, NH Big Tree for May By Anne Krantz, NH Big Tree Team,

    E-print Network

    New Hampshire, University of

    Apple Tree, NH Big Tree for May By Anne Krantz, NH Big Tree Team, UNH Cooperative Extension The explosion of apple blossoms in May transforms the most gnarled old tree into a delicate cloud of beauty (1817-1862) in his essay "The Wild Apple Tree," described the blossoms perfectly: `The flowers

  13. Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.)

    E-print Network

    Basheer-Salimia, Rezq; Awad, Murad; Ward, Joy K.

    2012-09-26

    the rest of the World [5]. In Palestine, fig trees are grown historically all over the country and are mostly located on marginal lands, in combination with other fruit trees (mainly olive and grape), or are scattered at the periphery of orchards..., random samples of 20 adult leaves and 20 mature fruits were collected from three adult trees per genotype. 15 leaf morphological and 26 pomological traits (Tables 4, 5, respectively) were determined according to the fig descriptors prepared by IPGRI...

  14. Relationship between fermented papaya preparation supplementation, erythrocyte integrity and antioxidant status in pre-diabetics.

    PubMed

    Somanah, Jhoti; Bourdon, Emmanuel; Rondeau, Philippe; Bahorun, Theeshan; Aruoma, Okezie I

    2014-03-01

    Erythrocytes and their membranes are favorable models to study the relationship between diabetes and susceptibility of erythrocytes to oxidative stress damage. The recommendation for the use of fermented papaya preparation (FPP) as a functional food for dietary management of type 2 diabetes was evaluated by assessing its effect on the human antioxidant status and erythrocyte integrity on a multi-ethnical pre-diabetic population. The in vivo effect of FPP was compared with its in vitro free radical scavenging potentials. FPP exhibited potent in vitro free radical scavenging activities thought to be attributed to residual phenolic or flavonoid compounds. Low doses of FPP significantly reduced the susceptibility of human erythrocytes to undergo free radical-induced hemolysis. The intake of 6g FPP/day for a period of 14weeks was observed to significantly reduce the rate of hemolysis and accumulation of protein carbonyls in the blood plasma of pre-diabetics. That FPP consumption on a daily basis can strengthen the antioxidant defense system in vivo was clearly demonstrated by the marked increase of total antioxidant status in the FPP-supplemented pre-diabetics. That FPP maintains the integrity of erythrocytes could benefit the strategies to improve the quality of future blood products. PMID:24316314

  15. Glycyl endopeptidase from papaya latex: partial purification and use for production of fish gelatin hydrolysate.

    PubMed

    Karnjanapratum, Supatra; Benjakul, Soottawat

    2014-12-15

    An aqueous two-phase system (ATPS) in combination with ammonium sulphate ((NH4)2SO4) precipitation was applied to fractionate glycyl endopeptidase from the papaya latex of Red Lady and Khack Dum cultivars. ATPS containing polyethylene glycol (PEG 2000 and 6000) and salts ((NH4)2SO4 and MgSO4) at different concentrations were used. Glycyl endopeptidase with high purification fold (PF) and yield was found in the salt-rich bottom phase of ATPS with 10%PEG 6000-10% (NH4)2SO4. When ATPS fraction from Red Lady cultivar was further precipitated with 40-60% saturation of (NH4)2SO4, PF of 2.1-fold with 80.23% yield was obtained. Almost all offensive odorous compounds, particularly benzyl isothiocyanate, were removed from partially purified glycyl endopeptidase (PPGE). The fish gelatin hydrolysates prepared using PPGE showed higher ABTS radical scavenging activity and less odour, compared with those of crude extract (CE). Thus antioxidative gelatin hydrolysate with negligible undesirable odour could be prepared with the aid of PPGE. PMID:25038693

  16. cDNA cloning of a novel gene codifying for the enzyme lycopene ?-cyclase from Ficus carica and its expression in Escherichia coli.

    PubMed

    Araya-Garay, José Miguel; Feijoo-Siota, Lucía; Veiga-Crespo, Patricia; Villa, Tomás González

    2011-11-01

    Lycopene beta-cyclase (?-LCY) is the key enzyme that modifies the linear lycopene molecule into cyclic ?-carotene, an indispensable carotenoid of the photosynthetic apparatus and an important source of vitamin A in human and animal nutrition. Owing to its antioxidant activity, it is commercially used in the cosmetic and pharmaceutical industries, as well as an additive in foodstuffs. Therefore, ?-carotene has a large share of the carotenoidic market. In this study, we used reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE)-PCR to obtain and clone a cDNA copy of the gene Lyc-? from Ficus carica (Lyc-? Fc), which codes for the enzyme lycopene ?-cyclase (?-LCY). Expression of this gene in Escherichia coli produced a single polypeptide of 56 kDa of weight, containing 496 amino acids, that was able to cycle both ends of the lycopene chain. Amino acid analysis revealed that the protein contained several conserved plant cyclase motifs. ?-LCY activity was revealed by heterologous complementation analysis, with lycopene being converted to ?-carotene as a result of the enzyme's action. The ?-LCY activity of the expressed protein was confirmed by high-performance liquid chromatography (HPLC) identification of the ?-carotene. The lycopene to ?-carotene conversion rate was 90%. The experiments carried out in this work showed that ?-LYC is the enzyme responsible for converting lycopene, an acyclic carotene, to ?-carotene, a bicyclic carotene in F. carica. Therefore, by cloning and expressing ?-LCY in E. coli, we have obtained a new gene for ?-carotene production or as part of the biosynthetic pathway of astaxanthin. So far, this is the first and only gene of the carotenoid pathway identified in F. carica. PMID:21792589

  17. Tree nut oils

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The major tree nuts include almonds, Brazil nuts, cashew nuts, hazelnuts, macadamia nuts, pecans, pine nuts, pistachio nuts, and walnuts. Tree nut oils are appreciated in food applications because of their flavors and are generally more expensive than other gourmet oils. Research during the last de...

  18. CSI for Trees

    ERIC Educational Resources Information Center

    Rubino, Darrin L.; Hanson, Deborah

    2009-01-01

    The circles and patterns in a tree's stem tell a story, but that story can be a mystery. Interpreting the story of tree rings provides a way to heighten the natural curiosity of students and help them gain insight into the interaction of elements in the environment. It also represents a wonderful opportunity to incorporate the nature of science.…

  19. Tree Topology Estimation

    PubMed Central

    Estrada, Rolando; Tomasi, Carlo; Schmidler, Scott C.; Farsiu, Sina

    2015-01-01

    Tree-like structures are fundamental in nature, and it is often useful to reconstruct the topology of a tree—what connects to what—from a two-dimensional image of it. However, the projected branches often cross in the image: the tree projects to a planar graph, and the inverse problem of reconstructing the topology of the tree from that of the graph is ill-posed. We regularize this problem with a generative, parametric tree-growth model. Under this model, reconstruction is possible in linear time if one knows the direction of each edge in the graph—which edge endpoint is closer to the root of the tree—but becomes NP-hard if the directions are not known. For the latter case, we present a heuristic search algorithm to estimate the most likely topology of a rooted, three-dimensional tree from a single two-dimensional image. Experimental results on retinal vessel, plant root, and synthetic tree datasets show that our methodology is both accurate and efficient. PMID:26353004

  20. TRANSPLANTING SHADE TREES.

    ERIC Educational Resources Information Center

    Illinois Univ., Urbana. Coll. of Agriculture.

    THIS RESOURCE MATERIAL FOR USE IN HIGH SCHOOL VOCATIONAL AGRICULTURE AND ADULT FARMER CLASSES WAS DESIGNED BY SUBJECT MATTER SPECIALISTS, TEACHER EDUCATORS, SUPERVISORS, AND TEACHERS. THE OBJECTIVE IS TO HELP SOLVE PROBLEMS IN TRANSPLANTING SHADE TREES. THE MAJOR SECTION TITLES ARE STATED AS PROBLEMS -- (1) HOW SHOULD I PREPARE A TREE FOR MOVING,…

  1. Trees for Mother Earth.

    ERIC Educational Resources Information Center

    Greer, Sandy

    1993-01-01

    Describes Trees for Mother Earth, a program in which secondary students raise funds to buy fruit trees to plant during visits to the Navajo Reservation. Benefits include developing feelings of self-worth among participants, promoting cultural exchange and understanding, and encouraging self-sufficiency among the Navajo. (LP)

  2. Trees in Our Lives.

    ERIC Educational Resources Information Center

    NatureScope, 1986

    1986-01-01

    Provides: (1) background information on how trees have influenced human history and how trees affect people today; (2) four activities dealing with these topics; and (3) a ready-to-copy page related to paper and plastics. Activities include an objective, recommended age level(s), subject area(s), list of materials needed, and procedures. (JN)

  3. Trees Are Terrific!

    ERIC Educational Resources Information Center

    Braus, Judy, Ed.

    1992-01-01

    Ranger Rick's NatureScope is a creative education series dedicated to inspiring in children an understanding and appreciation of the natural world while developing the skills they will need to make responsible decisions about the environment. Contents are organized into the following sections: (1) "What Makes a Tree a Tree?," including information…

  4. Biological control of toxigenic citrus and papaya-rotting fungi by Streptomyces violascens MT7 and its extracellular metabolites.

    PubMed

    Choudhary, Bharti; Nagpure, Anand; Gupta, Rajinder K

    2015-12-01

    An Indian indigenous, Loktak Lake soil isolate Streptomyces violascens MT7 was assessed for its biocontrol potential both in vitro and in vivo against toxigenic fruit-rotting fungi. Strain MT7 exhibited broad-spectrum antifungal activity against various pathogenic postharvest fungi of citrus and papaya. In shake-flask fermentation, antagonist S. violascens MT7 highly produced extracellular antifungal metabolites in early stationary growth phase in glucose-yeast extract-malt extract (M93) broth. Both extracellular culture fluid (ECF) and its n-butanol extract showed significant broad-spectrum fungal mycelial inhibition of several tested fruit-rotting fungi. Antifungal metabolite was found to be heat stable, nonpeptidic, and polyene type antibiotic. The lowest minimum inhibitory concentration (MIC) of n-butanol extract against Colletotrichum gloeosporioides MTCC 9664 and Aspergillus niger MTCC 281 was 0.0312 and 0.0625?mg/ml, respectively. Purification of n-butanol extract through silica gel chromatography resulted in partial purification of bioactive metabolite and the TLC autobiography revealed the presence of single antifungal metabolite with Rf value of 0.755. In vivo bioassays demonstrated the biocontrol potential of tested biocontrol agents on fruit-rotting fungi. Use of cell suspension of S. violascens MT7, extracellular metabolite(s), and n-butanol extract significantly (p?papaya fruits. Therefore, these results strongly suggest a high potential for application of S. violascens MT7 and its extracellular metabolites as an effective eco-friendly alternative to synthetic fungicides for controlling toxigenic citrus and papaya-rotting fungi. PMID:26214840

  5. Lazy decision trees

    SciTech Connect

    Friedman, J.H.; Yun, Yeogirl; Kohavi, R.

    1996-12-31

    Lazy learning algorithms, exemplified by nearest-neighbor algorithms, do not induce a concise hypothesis from a given training set; the inductive process is delayed until a test instance is given. Algorithms for constructing decision trees, such as C4.5, ID3, and CART create a single {open_quotes}best{close_quotes} decision tree during the training phase, and this tree is then used to classify test instances. The tests at the nodes of the constructed tree are good on average, but there may be better tests for classifying a specific instance. We propose a lazy decision tree algorithm-LazyDT-that conceptually constructs the {open_quotes}best{close_quote} decision tree for each test instance. In practice, only a path needs to be constructed, and a caching scheme makes the algorithm fast. The algorithm is robust with respect to missing values without resorting to the complicated methods usually seen in induction of decision trees. Experiments on real and artificial problems are presented.

  6. Phylogenetic trees in bioinformatics

    SciTech Connect

    Burr, Tom L

    2008-01-01

    Genetic data is often used to infer evolutionary relationships among a collection of viruses, bacteria, animal or plant species, or other operational taxonomic units (OTU). A phylogenetic tree depicts such relationships and provides a visual representation of the estimated branching order of the OTUs. Tree estimation is unique for several reasons, including: the types of data used to represent each OTU; the use ofprobabilistic nucleotide substitution models; the inference goals involving both tree topology and branch length, and the huge number of possible trees for a given sample of a very modest number of OTUs, which implies that fmding the best tree(s) to describe the genetic data for each OTU is computationally demanding. Bioinformatics is too large a field to review here. We focus on that aspect of bioinformatics that includes study of similarities in genetic data from multiple OTUs. Although research questions are diverse, a common underlying challenge is to estimate the evolutionary history of the OTUs. Therefore, this paper reviews the role of phylogenetic tree estimation in bioinformatics, available methods and software, and identifies areas for additional research and development.

  7. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Color-shifted complexes Weighted spanning tree enumerators of color-shifted complexes Ghodratollah Aalipour1,2 Art Duval1 1University in St. Louis October 20, 2013 Ghodratollah Aalipour, Art Duval Spanning tree enumerators of color

  8. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Critical group of graphs Critical group of simplicial complexes Spanning trees and the critical group of simplicial complexes Art Duval1 Mathematics Colloquium New Mexico State University October 20, 2011 Duval, Klivans, Martin Spanning trees

  9. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Critical group of graphs Critical group of simplicial complexes Spanning trees and the critical group of simplicial complexes Art Duval1 of Kansas Discrete CATS seminar University of Kentucky March 30, 2011 Duval, Klivans, Martin Spanning trees

  10. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Complete colorful complexes Weighted spanning tree enumerators of complete colorful complexes Ghodratollah Aalipour1,2 Art Duval1 1 of Mississippi March 2, 2013 Ghodratollah Aalipour, Art Duval Spanning tree enumerators of complete colorful

  11. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Complete colorful complexes Weighted spanning tree enumerators of complete colorful complexes Ghodratollah Aalipour1,2 Art Duval1 1 April 21, 2013 Ghodratollah Aalipour, Art Duval Spanning tree enumerators of complete colorful complexes

  12. Spanning trees of graphs Spanning trees of simplicial complexes

    E-print Network

    Duval, Art

    Spanning trees of graphs Spanning trees of simplicial complexes Critical group of graphs Critical group of simplicial complexes Spanning trees and the critical group of simplicial complexes Art Duval1 of Kansas Mathematics Seminar Reed College April 28, 2011 Duval, Klivans, Martin Spanning trees

  13. Global Tree Optimization: A Nongreedy Decision Tree Algorithm

    E-print Network

    Mitchell, John E.

    Global Tree Optimization: A Non­greedy Decision Tree Algorithm Kristin P. Bennett Email bennek­greedy approach for constructing globally optimal multivariate decision trees with fixed structure is pro­ posed­ rion at each decision node, typically one node at a time. In contrast, global tree optimization

  14. Evolutionary tree reconstruction

    NASA Technical Reports Server (NTRS)

    Cheeseman, Peter; Kanefsky, Bob

    1990-01-01

    It is described how Minimum Description Length (MDL) can be applied to the problem of DNA and protein evolutionary tree reconstruction. If there is a set of mutations that transform a common ancestor into a set of the known sequences, and this description is shorter than the information to encode the known sequences directly, then strong evidence for an evolutionary relationship has been found. A heuristic algorithm is described that searches for the simplest tree (smallest MDL) that finds close to optimal trees on the test data. Various ways of extending the MDL theory to more complex evolutionary relationships are discussed.

  15. Learning classification trees

    NASA Technical Reports Server (NTRS)

    Buntine, Wray

    1991-01-01

    Algorithms for learning classification trees have had successes in artificial intelligence and statistics over many years. How a tree learning algorithm can be derived from Bayesian decision theory is outlined. This introduces Bayesian techniques for splitting, smoothing, and tree averaging. The splitting rule turns out to be similar to Quinlan's information gain splitting rule, while smoothing and averaging replace pruning. Comparative experiments with reimplementations of a minimum encoding approach, Quinlan's C4 and Breiman et al. Cart show the full Bayesian algorithm is consistently as good, or more accurate than these other approaches though at a computational price.

  16. The gravity apple tree

    NASA Astrophysics Data System (ADS)

    Espinosa Aldama, Mariana

    2015-04-01

    The gravity apple tree is a genealogical tree of the gravitation theories developed during the past century. The graphic representation is full of information such as guides in heuristic principles, names of main proponents, dates and references for original articles (See under Supplementary Data for the graphic representation). This visual presentation and its particular classification allows a quick synthetic view for a plurality of theories, many of them well validated in the Solar System domain. Its diachronic structure organizes information in a shape of a tree following similarities through a formal concept analysis. It can be used for educational purposes or as a tool for philosophical discussion.

  17. The gene tree delusion.

    PubMed

    Springer, Mark S; Gatesy, John

    2016-01-01

    Higher-level relationships among placental mammals are mostly resolved, but several polytomies remain contentious. Song et al. (2012) claimed to have resolved three of these using shortcut coalescence methods (MP-EST, STAR) and further concluded that these methods, which assume no within-locus recombination, are required to unravel deep-level phylogenetic problems that have stymied concatenation. Here, we reanalyze Song et al.'s (2012) data and leverage these re-analyses to explore key issues in systematics including the recombination ratchet, gene tree stoichiometry, the proportion of gene tree incongruence that results from deep coalescence versus other factors, and simulations that compare the performance of coalescence and concatenation methods in species tree estimation. Song et al. (2012) reported an average locus length of 3.1kb for the 447 protein-coding genes in their phylogenomic dataset, but the true mean length of these loci (start codon to stop codon) is 139.6kb. Empirical estimates of recombination breakpoints in primates, coupled with consideration of the recombination ratchet, suggest that individual coalescence genes (c-genes) approach ?12bp or less for Song et al.'s (2012) dataset, three to four orders of magnitude shorter than the c-genes reported by these authors. This result has general implications for the application of coalescence methods in species tree estimation. We contend that it is illogical to apply coalescence methods to complete protein-coding sequences. Such analyses amalgamate c-genes with different evolutionary histories (i.e., exons separated by >100,000bp), distort true gene tree stoichiometry that is required for accurate species tree inference, and contradict the central rationale for applying coalescence methods to difficult phylogenetic problems. In addition, Song et al.'s (2012) dataset of 447 genes includes 21 loci with switched taxonomic names, eight duplicated loci, 26 loci with non-homologous sequences that are grossly misaligned, and numerous loci with >50% missing data for taxa that are misplaced in their gene trees. These problems were compounded by inadequate tree searches with nearest neighbor interchange branch swapping and inadvertent application of substitution models that did not account for among-site rate heterogeneity. Sixty-six gene trees imply unrealistic deep coalescences that exceed 100 million years (MY). Gene trees that were obtained with better justified models and search parameters show large increases in both likelihood scores and congruence. Coalescence analyses based on a curated set of 413 improved gene trees and a superior coalescence method (ASTRAL) support a Scandentia (treeshrews)+Glires (rabbits, rodents) clade, contradicting one of the three primary systematic conclusions of Song et al. (2012). Robust support for a Perissodactyla+Carnivora clade within Laurasiatheria is also lost, contradicting a second major conclusion of this study. Song et al.'s (2012) MP-EST species tree provided the basis for circular simulations that led these authors to conclude that the multispecies coalescent accounts for 77% of the gene tree conflicts in their dataset, but many internal branches of their MP-EST tree are stunted by an order of magnitude or more due to wholesale gene tree reconstruction errors. An independent assessment of branch lengths suggests the multispecies coalescent accounts for ?15% of the conflicts among Song et al.'s (2012) 447 gene trees. Unfortunately, Song et al.'s (2012) flawed phylogenomic dataset has been used as a model for additional simulation work that suggests the superiority of shortcut coalescence methods relative to concatenation. Investigator error was passed on to the subsequent simulation studies, which also incorporated further logical errors that should be avoided in future simulation studies. Illegitimate branch length switches in the simulation routines unfairly protected coalescence methods from their Achilles' heel, high gene tree reconstruction error at short internodes. These simulations therefore provide no

  18. Effectiveness of hydrothermal-calcium chloride treatment and chitosan on quality retention and microbial growth during storage of fresh-cut papaya.

    PubMed

    Ayón-Reyna, Lidia E; Tamayo-Limón, Ransés; Cárdenas-Torres, Feliznando; López-López, Martha E; López-Angulo, Gabriela; López-Moreno, Héctor S; López-Cervántes, Jaime; López-Valenzuela, José A; Vega-García, Misael O

    2015-03-01

    Rapid degradation of fresh-cut papaya limits its marketability. Hydrothermal treatments in combination with a calcium dip, applied to whole fruit before slicing, and also the application of chitosan as a coating film, have been found to have very good results in maintaining the quality of fresh-cut fruits. Based on these considerations, the aim of this study was to evaluate the effect of hydrothermal treatment (HT; 49 °C, 25 min) containing calcium chloride (Ca; 1%, w/v) followed by dipping in chitosan (Chit; 1%, w/v, 3 min) on the physical, chemical, and microbial qualities of papaya slices stored at 5 °C for 10 d. Pulp color, firmness, ascorbic acid, total phenolics, ?-carotene, and lycopene were evaluated every 2 d while the microbial quality (mesophilics, psychrophilics, molds, and yeasts) was evaluated every 5 d. Fruit treated with HT-Ca and HT-Ca + Chit showed better color and firmness retention than Control and Chit. Papaya slices treated with HT-Ca + Chit had higher nutritional content and lower microbial growth at the end of storage. The application of the HT-Ca + Chit could be used to reduce deterioration processes, maintaining physical, chemical, and microbial qualities and increasing the shelf life of fresh-cut papaya stored at 5 °C. PMID:25627320

  19. Ficus carica leaf extract modulates the lipid profile of rats fed with a high-fat diet through an increase of HDL-C.

    PubMed

    Joerin, Lorenz; Kauschka, Michaela; Bonnländer, Bernd; Pischel, Ivo; Benedek, Birgit; Butterweck, Veronika

    2014-02-01

    Ficus carica has been traditionally used for the treatment of several metabolic syndrome-related health problems. It was the objective of this study to investigate the preventive effects of a Ficus carica (FC) leaf extract on hyperlipidemia in high fat diet (HFD)-induced obese male rats. Male Sprague-Dawley rats (180-200 g) were fed with a regular diet, HFD or a HFD?+?oral treatment of either 50 mg/kg or 100 mg/kg of FC or 30 mg/kg pioglitazone for six weeks. A range of parameters was evaluated including body weight development, plasma levels of total cholesterol, triglycerides (TG), low-density-lipoprotein cholesterol, high-density lipoprotein cholesterol (HDL-C), adiponectin, leptin, glucose, insulin, interleukin-6 (IL-6), atherogenic index (AI) and the coronary risk index (CRI). FC significantly lowered TG and IL-6 levels and elevated HDL cholesterol (p?

  20. Changes in the phenolic and lipophilic composition, in the enzyme inhibition and antiproliferative activity of Ficus carica L. cultivar Dottato fruits during maturation.

    PubMed

    Marrelli, Mariangela; Menichini, Federica; Statti, Giancarlo A; Bonesi, Marco; Duez, Pierre; Menichini, Francesco; Conforti, Filomena

    2012-03-01

    Fruits of Ficus carica cultivar Dottato from Italy were examined to assess how the stage of ripeness influences their chemical composition, antioxidant activity, pancreatic lipase inhibition and antiproliferative properties on C32 melanoma cells. Fruits of the first harvest (June) showed a major content in furanocoumarins and pyranocoumarins whereas the fruits collected in September showed the highest polyphenolic content (11.9 mg/g of dried material). The total 70% ethanol extracts were portioned between methanol/water and n-hexane, dichloromethane and ethyl acetate, successively. Coumarins and fatty acid esters were the most abundant components of the n-hexane fractions. The dichloromethane fractions showed as major components 2 furanocoumarins (rutarenin and pimpinellin). The total extracts of F. carica cv. Dottato exhibited a significant dose-dependent antiradical and inhibition of lipid peroxidation activity, particularly fruits of the first harvest (June) that showed the highest activity with IC50 of 1.64 mg/mL and 0.004 mg/mL, respectively. Among single fractions, the ethyl acetate fraction from the second harvest (July) showed the highest antiradical activity with an IC50 value of 0.05 mg/mL while the dichloromethane fraction showed the best inhibition of lipid peroxidation with an IC50 value of 0.02 mg/mL. Dichloromethane fractions showed the highest photodynamic cytotoxicity with an IC50<5 ?g/mL. PMID:22222929

  1. Tree Disease Laboratory - 1 

    E-print Network

    Unknown

    2005-06-30

    Many homeowners need help caring for or removing damaged trees after a natural disaster. This publication explains what a certified arborist is and how to select one. It also cautions against burning debris downed by a storm....

  2. Tree-bank grammars

    SciTech Connect

    Charniak, E.

    1996-12-31

    By a {open_quotes}tree-bank grammar{close_quotes} we mean a context-free grammar created by reading the production rules directly from hand-parsed sentences in a tree bank. Common wisdom has it that such grammars do not perform well, though we know of no published data on the issue. The primary purpose of this paper is to show that the common wisdom is wrong. In particular, we present results on a tree-bank grammar based on the Penn Wall Street Journal tree bank. To the best of our knowledge, this grammar outperforms all other non-word-based statistical parsers/grammars on this corpus. That is, it outperforms parsers that consider the input as a string of tags and ignore the actual words of the corpus.

  3. Tea tree oil

    MedlinePLUS

    ... the skin (used topically) for infections such as acne, fungal infections of the nail (onychomycosis), lice, scabies, ... two months has no benefit. Mild to moderate acne. Applying a 5% tea tree oil gel appears ...

  4. Structural Equation Model Trees

    PubMed Central

    Brandmaier, Andreas M.; von Oertzen, Timo; McArdle, John J.; Lindenberger, Ulman

    2015-01-01

    In the behavioral and social sciences, structural equation models (SEMs) have become widely accepted as a modeling tool for the relation between latent and observed variables. SEMs can be seen as a unification of several multivariate analysis techniques. SEM Trees combine the strengths of SEMs and the decision tree paradigm by building tree structures that separate a data set recursively into subsets with significantly different parameter estimates in a SEM. SEM Trees provide means for finding covariates and covariate interactions that predict differences in structural parameters in observed as well as in latent space and facilitate theory-guided exploration of empirical data. We describe the methodology, discuss theoretical and practical implications, and demonstrate applications to a factor model and a linear growth curve model. PMID:22984789

  5. Tea tree oil

    MedlinePLUS

    ... infection as medications such as clotrimazole or terbinafine. Fungus infections of the nails (onychomycosis). Topical application of ... in tea tree oil may kill bacteria and fungus, and reduce allergic skin reactions.

  6. Leonardo's Tree Theory.

    ERIC Educational Resources Information Center

    Werner, Suzanne K.

    2003-01-01

    Describes a series of activities exploring Leonardo da Vinci's tree theory that are designed to strengthen 8th grade students' data collection and problem solving skills in physical science classes. (KHR)

  7. Tea tree oil.

    PubMed

    Larson, David; Jacob, Sharon E

    2012-01-01

    Tea tree oil is an increasingly popular ingredient in a variety of household and cosmetic products, including shampoos, massage oils, skin and nail creams, and laundry detergents. Known for its potential antiseptic properties, it has been shown to be active against a variety of bacteria, fungi, viruses, and mites. The oil is extracted from the leaves of the tea tree via steam distillation. This essential oil possesses a sharp camphoraceous odor followed by a menthol-like cooling sensation. Most commonly an ingredient in topical products, it is used at a concentration of 5% to 10%. Even at this concentration, it has been reported to induce contact sensitization and allergic contact dermatitis reactions. In 1999, tea tree oil was added to the North American Contact Dermatitis Group screening panel. The latest prevalence rates suggest that 1.4% of patients referred for patch testing had a positive reaction to tea tree oil. PMID:22653070

  8. Generalized constructive tree weights

    SciTech Connect

    Rivasseau, Vincent E-mail: adrian.tanasa@ens-lyon.org; Tanasa, Adrian E-mail: adrian.tanasa@ens-lyon.org

    2014-04-15

    The Loop Vertex Expansion (LVE) is a quantum field theory (QFT) method which explicitly computes the Borel sum of Feynman perturbation series. This LVE relies in a crucial way on symmetric tree weights which define a measure on the set of spanning trees of any connected graph. In this paper we generalize this method by defining new tree weights. They depend on the choice of a partition of a set of vertices of the graph, and when the partition is non-trivial, they are no longer symmetric under permutation of vertices. Nevertheless we prove they have the required positivity property to lead to a convergent LVE; in fact we formulate this positivity property precisely for the first time. Our generalized tree weights are inspired by the Brydges-Battle-Federbush work on cluster expansions and could be particularly suited to the computation of connected functions in QFT. Several concrete examples are explicitly given.

  9. Combining Binary Search Trees

    E-print Network

    Demaine, Erik D.

    We present a general transformation for combining a constant number of binary search tree data structures (BSTs) into a single BST whose running time is within a constant factor of the minimum of any “well-behaved” bound ...

  10. Construction of Decision Trees

    E-print Network

    Banks, Edwin Roger

    1970-02-01

    The construction of optimal decision trees for the problem stated within can be accomplished by an exhaustive enumeration. This paper discusses two approaches. The section on heuristic methods gives mostly negative results ...

  11. Trees as energy crops

    SciTech Connect

    Standiford, R.B.; Donaldson, D.R.

    1982-01-01

    A report is presented of studies on the energy production of forest trees in California. The volume yield of the fastest growing species (Eucalyptus) was 23 cubic metres per hectare and energy yield 20 million kcal per hectare per annum.

  12. TREE TRAILS Career Cards

    E-print Network

    Specialist Wildland Firefighter Wildlife Biologist Writer #12;Module 4 TREE TRAILS Instructional Procedures that uses computers to create and maintain data and maps Wildland Firefighter a person puts out a fire

  13. How Trees Can Save Energy.

    ERIC Educational Resources Information Center

    Fazio, James R., Ed.

    1991-01-01

    This document might easily have been called "How To Use Trees To Save Energy". It presents the energy saving advantages of landscaping the home and community with trees. The discussion includes: (1) landscaping advice to obtain the benefits of tree shade; (2) the heat island phenomenon in cities; (3) how and where to properly plant trees for…

  14. Jennifer Ramos The Notable Trees

    E-print Network

    Weston, Nathaniel B.

    Jennifer Ramos The Notable Trees of Villanova University The Importance of VU's Urban Trees Urban trees serve a number of important purposes: they reduce air and noise pollution, reduce soil erosion to sustainability, the campus's many trees play an important role in working towards this commitment. However, even

  15. COMPONENT User's Guide Consensus trees

    E-print Network

    Page, Roderic

    COMPONENT User's Guide Chapter 4 Consensus trees Consensus trees are a convenient way to summarise the agreement between two or more trees. This chapter describes the consensus methods available in COMPONENT subtree (Kubicka et al, 1992; also called the common pruned tree [Finden and Gordon, 1985]). This method

  16. TREES of SCa Public Service of Clemson University S.C. Champion Tree Nomination Form

    E-print Network

    Stuart, Steven J.

    CHAMPION TREES of SCa Public Service of Clemson University S.C. Champion Tree Nomination Form Today's date: ___________________________ Scientific tree name: ________________________________________________ Common tree name: _________________________________________________ Group

  17. Zucchini tigré mosaic virus is a distinct potyvirus in the papaya ringspot virus cluster: molecular and biological insights.

    PubMed

    Romay, G; Lecoq, H; Desbiez, C

    2014-02-01

    In recent years, three new potyviruses have been described in the papaya ringspot virus (PRSV) cluster. In addition, two types of PRSV are recognized, type W, infecting cucurbit plants, and type P, infecting papaya and also cucurbits. A third type, PRSV-T, was also partially described in Guadeloupe. Complete genome sequencing of four PRSV-T isolates showed that this virus is a related virus that is distinct from PRSV, and the name zucchini tigré mosaic virus (ZTMV) is proposed, in reference to the typical symptoms observed in zucchini squash. Eleven other viral isolates from different geographic origins were confirmed as ZTMV isolates using the complete sequence of the cylindrical inclusion (CI) coding region, whereas pairwise sequence similarities in the coat protein (CP) coding region did not unambiguously distinguish ZTMV isolates from PRSV isolates. The use of the CI coding region for species demarcation appears more suitable than the CP coding region for closely related viruses. Principal coordinates analysis based on the biological behavior of the viral isolates studied clustered PRSV-P, PRSV-W and ZTMV isolates into three different groups. Therefore, ZTMV is different from PRSV in its molecular and biological properties. PMID:23979176

  18. Collaborative trial for the validation of event-specific PCR detection methods of genetically modified papaya Huanong No.1.

    PubMed

    Wei, Jiaojun; Le, Huangying; Pan, Aihu; Xu, Junfeng; Li, Feiwu; Li, Xiang; Quan, Sheng; Guo, Jinchao; Yang, Litao

    2016-03-01

    For transferring the event-specific PCR methods of genetically modified papaya Huanong No.1 to other laboratories, we validated the previous developed PCR assays of Huanong No.1 according to the international standard organization (ISO) guidelines. A total of 11 laboratories participated and returned their test results in this trial. In qualitative PCR assay, the high specificity and limit of detection as low as 0.1% was confirmed. For the quantitative PCR assay, the limit of quantification was as low as 25 copies. The quantitative biases among ten blind samples were within the range between 0.21% and 10.04%. Furthermore, the measurement uncertainty of the quantitative PCR results was calculated within the range between 0.28% and 2.92% for these ten samples. All results demonstrated that the Huanong No.1 qualitative and quantitative PCR assays were creditable and applicable for identification and quantification of GM papaya Huanong No.1 in further routine lab analysis. PMID:26471522

  19. Comparison of transmission of Papaya leaf curl China virus among four cryptic species of the whitefly Bemisia tabaci complex

    PubMed Central

    Guo, Tao; Guo, Qi; Cui, Xi-Yun; Liu, Yin-Quan; Hu, Jian; Liu, Shu-Sheng

    2015-01-01

    Begomoviruses are transmitted by cryptic species of the whitefly Bemisia tabaci complex, often in a species-specific manner. Papaya leaf curl China virus (PaLCuCNV) has been recorded to infect several crops including papaya, tomato and tobacco in China. To help assess the risks of spread of this virus, we compared the acquisition, retention and transmission of PaLCuCNV among four species of whiteflies, Middle East-Asia Minor 1 (MEAM1), Mediterranean (MED), Asia 1 and Asia II 7. All four species of whiteflies are able to acquire, retain and transmit the virus, but with different levels of efficiency. Transmission tests using tomato as the host plant showed that MEAM1 transmitted PaLCuCNV with substantially higher efficiency than did MED, Asia 1 and Asia II 7. Furthermore, accumulation of PaLCuCNV in the whiteflies was positively associated with its efficiency of transmitting the virus. Altogether, these findings indicate that MEAM1 is the most efficient vector for PaLCuCNV in the four species of whiteflies, and suggest that risks of PaLCuCNV pandemics are high in regions where MEAM1 occurs. PMID:26486606

  20. TREE TRAILS Trees fascinate us because the oldest among them span many human generations. Trees can be a living

    E-print Network

    1 TREE TRAILS Trees fascinate us because the oldest among them span many human generations. Trees to important events or people in the community. Tree Trails Tree History Goal: Students will research the history of a tree(s) and make connections to the past. Objectives: Students will 1. Investigate a specific

  1. Nearest tree Always go to the closest

    E-print Network

    Nearest tree Always go to the closest tree Largest tree Always go to the tree with the most apples Optimal 1-step forager Maximize rate (= ratio of apples to travel time), but only consider one tree at a time # apples travel time Optimal 2-, 3-step forager Maximize rate over 2-tree (or 3-tree) paths

  2. In this chapter we study infinite trees. The main things we look at are Konig's tree theorem, Aronszajn trees, and Suslin trees.

    E-print Network

    Monk, Don

    14. Trees In this chapter we study infinite trees. The main things we look at are K¨onig's tree theorem, Aronszajn trees, and Suslin trees. A tree is a partially ordered set (T, t T, the set {s T : s tree

  3. Deletion from Okasaki's Red-Black Trees

    E-print Network

    Might, Matt

    Deletion from Okasaki's Red-Black Trees: A Functional Pearl Matt Might University of Utah matt;(: balance : (All (A) ((RBTree A) -> (RBTree A)))) (define (balance tree) (RBTree (RBTree-comparer tree) (balance-helper (RBTree-tree tree)))) (: balance-helper : (All (A) ((Tree A) -> (Tree A)))) (define

  4. A novel form of ficin from Ficus carica latex: Purification and characterization.

    PubMed

    Baeyens-Volant, Danielle; Matagne, André; El Mahyaoui, Rachida; Wattiez, Ruddy; Azarkan, Mohamed

    2015-09-01

    A novel ficin form, named ficin E, was purified from fig tree latex by a combination of cation-exchange chromatography on SP-Sepharose Fast Flow, Thiopropyl Sepharose 4B and fplc-gel filtration chromatography. The new ficin appeared not to be sensitive to thiol derivatization by a polyethylene glycol derivative, allowing its purification. The protease is homogeneous according to PAGE, SDS-PAGE, mass spectrometry, N-terminal micro-sequencing analyses and E-64 active site titration. N-terminal sequencing of the first ten residues has shown high identity with the other known ficin (iso)forms. The molecular weight was found to be (24,294±10)Da by mass spectrometry, a lower value than the apparent molecular weight observed on SDS-PAGE, around 27 kDa. Far-UV CD data revealed a secondary structure content of 22% ?-helix and 26% ?-sheet. The protein is not glycosylated as shown by carbohydrate analysis. pH and temperature measurements indicated maxima activity at pH 6.0 and 50 °C, respectively. Preliminary pH stability analyses have shown that the protease conserved its compact structure in slightly acidic, neutral and alkaline media but at acidic pH (<3), the formation of some relaxed or molten state was evidenced by 8-anilino-1-naphtalenesulfonic acid binding characteristics. Comparison with the known ficins A, B, C, D1 and D2 (iso)forms revealed that ficin E showed activity profile that looked like ficin A against two chromogenic substrates while it resembled ficins D1 and D2 against three fluorogenic substrates. Enzymatic activity of ficin E was not affected by Mg(2+), Ca(2+) and Mn(2+) at a concentration up to 10mM. However, the activity was completely suppressed by Zn(2+) at a concentration of 1mM. Inhibitory activity measurements clearly identified the enzyme as a cysteine protease, being unaffected by synthetic (Pefabloc SC, benzamidine) and by natural proteinaceous (aprotinin) serine proteases inhibitors, by aspartic proteases inhibitors (pepstatin A) and by metallo-proteases inhibitors (EDTA, EGTA). Surprisingly, it was well affected by the metallo-protease inhibitor o-phenanthroline. The enzymatic activity was however completely blocked by cysteine proteases inhibitors (E-64, iodoacetamide), by thiol-blocking compounds (HgCl2) and by cysteine/serine proteases inhibitors (TLCK and TPCK). This is a novel ficin form according to peptide mass fingerprint analysis, specific amidase activity, SDS-PAGE and PAGE electrophoretic mobility, N-terminal sequencing and unproneness to thiol pegylation. PMID:26083455

  5. Odds-On Trees

    E-print Network

    Bose, Prosenjit; Douieb, Karim; Dujmovic, Vida; King, James; Morin, Pat

    2010-01-01

    Let R^d -> A be a query problem over R^d for which there exists a data structure S that can compute P(q) in O(log n) time for any query point q in R^d. Let D be a probability measure over R^d representing a distribution of queries. We describe a data structure called the odds-on tree, of size O(n^\\epsilon) that can be used as a filter that quickly computes P(q) for some query values q in R^d and relies on S for the remaining queries. With an odds-on tree, the expected query time for a point drawn according to D is O(H*+1), where H* is a lower-bound on the expected cost of any linear decision tree that solves P. Odds-on trees have a number of applications, including distribution-sensitive data structures for point location in 2-d, point-in-polytope testing in d dimensions, ray shooting in simple polygons, ray shooting in polytopes, nearest-neighbour queries in R^d, point-location in arrangements of hyperplanes in R^d, and many other geometric searching problems that can be solved in the linear-decision tree mo...

  6. Tree nut allergens.

    PubMed

    Roux, Kenneth H; Teuber, Suzanne S; Sathe, Shridhar K

    2003-08-01

    Allergic reactions to tree nuts can be serious and life threatening. Considerable research has been conducted in recent years in an attempt to characterize those allergens that are most responsible for allergy sensitization and triggering. Both native and recombinant nut allergens have been identified and characterized and, for some, the IgE-reactive epitopes described. Some allergens, such as lipid transfer proteins, profilins, and members of the Bet v 1-related family, represent minor constituents in tree nuts. These allergens are frequently cross-reactive with other food and pollen homologues, and are considered panallergens. Others, such as legumins, vicilins, and 2S albumins, represent major seed storage protein constituents of the nuts. The allergenic tree nuts discussed in this review include those most commonly responsible for allergic reactions such as hazelnut, walnut, cashew, and almond as well as those less frequently associated with allergies including pecan, chestnut, Brazil nut, pine nut, macadamia nut, pistachio, coconut, Nangai nut, and acorn. PMID:12915766

  7. The Di erences Between Kurepa Trees And Jech Kunen Trees 1 Renling Jin

    E-print Network

    Jin, Renling

    The Di erences Between Kurepa Trees And Jech Kunen Trees 1 Renling Jin Abstract By an !1 tree we mean a tree of power !1 and height !1. An !1 tree is called a Kurepa tree if all its levels are countable and it has more than !1 branches. An !1 tree is called a Jech Kunen tree if it has branches

  8. Heartwood and tree exudates

    SciTech Connect

    Hillis, W.E.

    1987-01-01

    Increasingly, mankind will depend on renewable resources produced at low energy cost - such as forest products. Greater demands will require increased growth as well as utilisation with reduced loss. After a certain age, trees from heartwood containing increased amounts of extractives which are also formed in injured sapwood or are exuded. Their presence can provide trees with resistance to disease and insect attack and they can also affect the efficient utilisation of wood. In this book different facets of heartwood, extractives and exudates are reviewed as a whole for the first time.

  9. A Parallel Tree Code

    E-print Network

    John Dubinski

    1996-03-18

    We describe a new implementation of a parallel N-body tree code. The code is load-balanced using the method of orthogonal recursive bisection to subdivide the N-body system into independent rectangular volumes each of which is mapped to a processor on a parallel computer. On the Cray T3D, the load balance in the range of 70-90\\% depending on the problem size and number of processors. The code can handle simulations with $>$ 10 million particles roughly a factor of 10 greater than allowed in vectorized tree codes.

  10. Optimal Key-Trees for Tree-Based Private Authentication

    E-print Network

    Bencsáth, Boldizsár

    Optimal Key-Trees for Tree-Based Private Authentication Levente Butty´an, Tam´as Holczer, and Istv University of Technology and Economics, Hungary {buttyan, holczer, vajda}@crysys.hu Abstract. Key-tree based the problem of pri- vacy preserving authentication based on symmetric key cryptography. However, in the key

  11. WHAT DO YOU USE TREES FOR? Tree: Paper Birch

    E-print Network

    Ashline, George

    WHAT DO YOU USE TREES FOR? Tree: Paper Birch Scientific Name: Betula Papyrifera Tree# & Location a bark rubbing of the Paper Birch, then brainstorm and create a list of how we can use this bark the Paper Birch outside of the McCarthy Arts Center. · Tape a blank sheet of paper to the bark. · With your

  12. Tree Cross Sections The study of tree rings is called

    E-print Network

    , possibly from not enough water, sunlight, space or nutrients. 2. This tree shows scars where branches have Sections 3. Wide rings could mean the available water, sunlight, space or nutrients allowed the tree fungus is also evident in the sapwood. The fungus is carried by bark beetles and quickens the tree

  13. Can These Trees Be Saved? 

    E-print Network

    Taylor, Eric; Foster, C. Darwin

    2005-10-19

    This publication explains how to determine whether a storm-damaged tree can be saved. If the damage is not severe, most trees will recover in time. Helpful illustrations depict different levels of damage....

  14. FRACTAL TREES WITH SIDE BRANCHING

    E-print Network

    1998-01-10

    system for side branching is introduced, along with the Tokunaga self-similarity ... plants and trees, root systems, bronchial systems, ..... central node to the centers of the eight cubes. Due .... evolutionary trees, the nervous system, and com-.

  15. Flat Tree Oyster (Isognomon alatus)

    USGS Multimedia Gallery

    At high tide, the red bark of the Red Mangrove Trees is submerged, creating a brilliant reflection at the water's surface. Flat Tree Oysters (Isognomon alatus) grow on the upper portions of the roots....

  16. Simplicial matrix-tree theorems

    E-print Network

    Duval, Art M.; Klivans, Caroline J.; Martin, Jeremy L.

    2009-01-01

    We generalize the definition and enumeration of spanning trees from the setting of graphs to that of arbitrary-dimensional simplicial complexes ?, extending an idea due to G. Kalai. We prove a simplicial version of the Matrix-Tree Theorem...

  17. The Fig Tree's Lessons.

    ERIC Educational Resources Information Center

    Hall, Kristen

    1988-01-01

    Describes the dramatization of Katherine Anne Porter's story,"The Fig Tree's Lessons." Discusses the process by which this story was made accessible to an audience of 8- to 12-year-old children. Notes that this program attempts to avoid the simplification of the profound human experience of dealing with death. (KO)

  18. Digging Deeper with Trees.

    ERIC Educational Resources Information Center

    Growing Ideas, 2001

    2001-01-01

    Describes hands-on science areas that focus on trees. A project on leaf pigmentation involves putting crushed leaves in a test tube with solvent acetone to dissolve pigment. In another project, students learn taxonomy by sorting and classifying leaves based on observable characteristics. Includes a language arts connection. (PVD)

  19. Tree-Ties.

    ERIC Educational Resources Information Center

    Gresczyk, Rick

    Created to help students understand how plants were used for food, for medicine, and for arts and crafts among the Ojibwe (Chippewa) Indians, the game Tree-Ties combines earth and social sciences within a specific culture. The game requires mutual respect, understanding, and agreement to succeed. Sounding like the word "treaties", the title is a…

  20. Phylogenics & Tree-Thinking

    ERIC Educational Resources Information Center

    Baum, David A.; Offner, Susan

    2008-01-01

    Phylogenetic trees, which are depictions of the inferred evolutionary relationships among a set of species, now permeate almost all branches of biology and are appearing in increasing numbers in biology textbooks. While few state standards explicitly require knowledge of phylogenetics, most require some knowledge of evolutionary biology, and many…

  1. Arbutus unedo, Strawberry Tree

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The Encylopedia of Fruit and Nuts is designed as a research reference source on temperate and tropical fruit and nut crops. Strawberry tree or madrone is native to the Mediterranean region of southern Europe (Arbutus unedo L., Ericaceae) with a relict population in Ireland, as well as in North Ameri...

  2. Advisory Note TREE HEALTH

    E-print Network

    pest Advisory Note TREE HEALTH March 2007 Gypsy Moth Lymantria dispar (Lepidoptera: Lymantridae) Gypsy's. When fully grown, gypsy moth larvae (caterpillars) can be up to 70 mm long. They will feed for some people. Life Cycle 1. Fully grown larva feeding on oak Larvae: April- August Gypsy moth larvae

  3. Christmas Tree Category Manual.

    ERIC Educational Resources Information Center

    Bowman, James S.; Turmel, Jon P.

    This manual provides information needed to meet the standards for pesticide applicator certification. Pests and diseases of christmas tree plantations are identified and discussed. Section one deals with weeds and woody plants and the application, formulation and effects of herbicides in controlling them. Section two discusses specific diseases…

  4. Rate of tree carbon accumulation increases continuously with tree size

    USGS Publications Warehouse

    Stephenson, N.L.; Das, A.J.; Condit, R.; Russo, S.E.; Baker, P.J.; Beckman, N.G.; Coomes, D.A.; Lines, E.R.; Morris, W.K.; Rüger, N.; Álvarez, E.; Blundo, C.; Bunyavejchewin, S.; Chuyong, G.; Davies, S.J.; Duque, Á.; Ewango, C.N.; Flores, O.; Franklin, J.F.; Grau, H.R.; Hao, Z.; Harmon, M.E.; Hubbell, S.P.; Kenfack, D.; Lin, Y.; Makana, J.-R.; Malizia, A.; Malizia, L.R.; Pabst, R.J.; Pongpattananurak, N.; Su, S.-H.; Sun, I-F.; Tan, S.; Thomas, D.; van Mantgem, P.J.; Wang, X.; Wiser, S.K.; Zavala, M.A.

    2014-01-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle—particularly net primary productivity and carbon storage—increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree’s total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to understand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence.

  5. The effects of Ficus carica polysaccharide on immune response and expression of some immune-related genes in grass carp, Ctenopharyngodon idella.

    PubMed

    Yang, Xia; Guo, Jian Lin; Ye, Jin Yun; Zhang, Yi Xiang; Wang, Wei

    2015-01-01

    The present study investigated the effect of Ficus carica polysaccharide (FCP), isolated from the fruit of F. carica L., at 0%, 0.1%, 0.5% and 1.0% doses supplementation with feed on genes Interleukin 1-? (IL-1?), Tumor Necrosis Factor ? (TNF-?) and heat shock protein 70 (HSP70) gene expression in blood, humoral innate immune parameters and resistant to Flavobacterium columnare of grass carp at weeks 1, 2 and 3. The results revealed that administration of FCP significantly (P<0.05) up regulated IL-1? and TNF-? gene expression. HSP70 gene expression was significantly (P<0.05) lower in FCP-fed fish at the end of trial. The serum total protein, albumin and globulin did not significantly increased in any diet on the first week whereas it was significantly enhanced in 0.5% and 1.0% supplementation diets on weeks 2 and 3 when compared to control. The serum complement C3 was significantly (P<0.05) increased on weeks 1 and 2 when compared to control, however, no significant difference was found in this activity after 3 weeks of treatment. All diets significantly enhanced the serum lysozyme activity, bactericidal activity from weeks 1-2 as compared to control. Grass carp fed with FCP showed remarkably higher resistance against F. columnare (60% survival) compared to the control group (30% survival). These results confirm that FCP can up regulate immune related genes expression, stimulates immune response that per se enhances disease resistance in grass carp. PMID:25449378

  6. The Hopi Fruit Tree Book.

    ERIC Educational Resources Information Center

    Nyhuis, Jane

    Referring as often as possible to traditional Hopi practices and to materials readily available on the reservation, the illustrated booklet provides information on the care and maintenance of young fruit trees. An introduction to fruit trees explains the special characteristics of new trees, e.g., grafting, planting pits, and watering. The…

  7. Building up rhetorical structure trees

    SciTech Connect

    Marcu, D.

    1996-12-31

    I use the distinction between the nuclei and the satellites that pertain to discourse relations to introduce a compositionality criterion for discourse trees. I provide a first-order formalization of rhetorical structure trees and, on its basis, I derive an algorithm that constructs all the valid rhetorical trees that can be associated with a given discourse.

  8. Machine Learning Srihari Decision Trees

    E-print Network

    Machine Learning Srihari 1 Decision Trees Sargur Srihari srihari@cedar.buffalo.edu #12;Machine the unobserved examples · Inductive bias is a preference for small trees over large trees #12;Machine Learning #12;Machine Learning Srihari 4 Learning Data Day Outlook Temp Humidity Wind PlayTennis D1 Sunny Hot

  9. Our Air: Unfit for Trees.

    ERIC Educational Resources Information Center

    Dochinger, Leon S.

    To help urban, suburban, and rural tree owners know about air pollution's effects on trees and their tolerance and intolerance to pollutants, the USDA Forest Service has prepared this booklet. It answers the following questions about atmospheric pollution: Where does it come from? What can it do to trees? and What can we do about it? In addition,…

  10. Genetic conservation of native trees

    E-print Network

    Genetic conservation of native trees Scott McG. Wilson1 and C. J. A. Samuel Over recent years, the genetic conservation of British native tree populations has become an increasingly important part of the work of Tree Improvement Branch (TIB). This has been in response to a greater interest from forestry

  11. Singular spectrum for radial trees

    E-print Network

    Jonathan Breuer; Rupert L. Frank

    2008-06-03

    We prove several results showing that absolutely continuous spectrum for the Laplacian on radial trees is a rare event. In particular, we show that metric trees with unbounded edges have purely singular spectrum and that generically (in the sense of Baire) radial trees have purely singular continuous spectrum.

  12. Orthogonal Decision Trees Hillol Kargupta

    E-print Network

    Kargupta, Hilol

    1 Orthogonal Decision Trees Hillol Kargupta ¢¡ , Byung-Hoon Park £ , Haimonti Dutta Department orthogonal decision trees that offer an effective way to construct a redundancy- free, accurate, and meaningful representation of large decision-tree-ensembles often created by popular techniques

  13. Game Trees for Decision Analysis

    E-print Network

    Shenoy, Prakash P.

    1998-04-01

    Game trees (or extensive-form games) were first defined by von Neumann and Morgenstern in 1944. In this paper, we examine the use of game trees for representing Bayesian decision problems. We propose a method for solving game trees using local...

  14. Orthogonal Decision Trees Hillol Kargupta

    E-print Network

    Kargupta, Hilol

    Orthogonal Decision Trees Hillol Kargupta and Haimonti Dutta Department of Computer Science¡ hillol, hdutta1¢ @cs.umbc.edu Abstract This paper introduces orthogonal decision trees that of- fer an effective way to construct a redundancy-free, accu- rate, and meaningful representation of large decision-tree

  15. Weathering and chemical degradation of Methyl Eugenol and Raspberry Ketone solid dispensers for detection, monitoring and male annihilation of Bactrocera dorsalis (Hendel) and Bactrocera cucurbitae (Coquillett)(Diptera:Tephri

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Solid male lure dispensers containing methyl eugenol (ME) and raspberry ketone (RK), or mixtures of the lures (ME + RK), and dimethyl dichloro-vinyl phosphate (DDVP) were evaluated in AWPM bucket or Jackson traps in commercial papaya (Carica papaya L.) orchards where both oriental fruit fly, Bactroc...

  16. Genome size variation among sex types in dioecious and triecious Caricaceae species

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Caricaceae is a small family consisting of 35 species of varying sexual systems and includes economically important fruit crop, Carica papaya, and other species of “highland papayas”. Flow cytometry was used to obtain genome sizes for 11 species in three genera of Caricaceae to determine if genome s...

  17. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya. The food-grade product is obtained by repeated filtration of the crude latex or an aqueous solution of latex or by precipitation from an aqueous solution of latex. The resulting enzyme preparation may...

  18. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ....1585 Papain. (a) Papain (CAS Reg. No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya. The food-grade product is... an aqueous solution of latex. The resulting enzyme preparation may be used in a liquid or dry...

  19. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Substances Affirmed as GRAS § 184.1585 Papain. (a) Papain (CAS Reg. No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya... latex or by precipitation from an aqueous solution of latex. The resulting enzyme preparation may...

  20. 21 CFR 184.1585 - Papain.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Substances Affirmed as GRAS § 184.1585 Papain. (a) Papain (CAS Reg. No. 9001-73-4) is a proteolytic enzyme derived from Carica papaya L. Crude latex containing the enzyme is collected from slashed unripe papaya... latex or by precipitation from an aqueous solution of latex. The resulting enzyme preparation may...

  1. van Emde Boas Trees -Dynamic Predecessors

    E-print Network

    Zwick, Uri

    van Emde Boas Trees - Dynamic Predecessors Uri Zwick Tel Aviv University #12;Insert Delete Find > } = | , = | = #12;van Emde Boas Trees A vEB tree representing consists of: ­ word size ­ the minimal keyEB tree for ( - , , ) #12;van Emde Boas Trees Are , better names than , ? #12;van Emde Boas Trees

  2. Red-black Tree Jingjing Xia

    E-print Network

    Chen, Yangjun

    Advanced Algorithm Design Red-black Tree Jingjing Xia #12;Red-Black Tree A red-black tree is a binary search tree, and each node contains one extra field: its color, it can be either black or red of the binary search tree. If a binary search tree satisfies all the following red-black properties, it is a red-black

  3. Gene tree correction for reconciliation and species tree inference

    PubMed Central

    2012-01-01

    Background Reconciliation is the commonly used method for inferring the evolutionary scenario for a gene family. It consists in “embedding” inferred gene trees into a known species tree, revealing the evolution of the gene family by duplications and losses. When a species tree is not known, a natural algorithmic problem is to infer a species tree from a set of gene trees, such that the corresponding reconciliation minimizes the number of duplications and/or losses. The main drawback of reconciliation is that the inferred evolutionary scenario is strongly dependent on the considered gene trees, as few misplaced leaves may lead to a completely different history, with significantly more duplications and losses. Results In this paper, we take advantage of certain gene trees’ properties in order to preprocess them for reconciliation or species tree inference. We flag certain duplication vertices of a gene tree, the “non-apparent duplication” (NAD) vertices, as resulting from the misplacement of leaves. In the case of species tree inference, we develop a polynomial-time heuristic for removing the minimum number of species leading to a set of gene trees that exhibit no NAD vertices with respect to at least one species tree. In the case of reconciliation, we consider the optimization problem of removing the minimum number of leaves or species leading to a tree without any NAD vertex. We develop a polynomial-time algorithm that is exact for two special classes of gene trees, and show a good performance on simulated data sets in the general case. PMID:23167951

  4. Rate of tree carbon accumulation increases continuously with tree size.

    PubMed

    Stephenson, N L; Das, A J; Condit, R; Russo, S E; Baker, P J; Beckman, N G; Coomes, D A; Lines, E R; Morris, W K; Rüger, N; Alvarez, E; Blundo, C; Bunyavejchewin, S; Chuyong, G; Davies, S J; Duque, A; Ewango, C N; Flores, O; Franklin, J F; Grau, H R; Hao, Z; Harmon, M E; Hubbell, S P; Kenfack, D; Lin, Y; Makana, J-R; Malizia, A; Malizia, L R; Pabst, R J; Pongpattananurak, N; Su, S-H; Sun, I-F; Tan, S; Thomas, D; van Mantgem, P J; Wang, X; Wiser, S K; Zavala, M A

    2014-03-01

    Forests are major components of the global carbon cycle, providing substantial feedback to atmospheric greenhouse gas concentrations. Our ability to understand and predict changes in the forest carbon cycle--particularly net primary productivity and carbon storage--increasingly relies on models that represent biological processes across several scales of biological organization, from tree leaves to forest stands. Yet, despite advances in our understanding of productivity at the scales of leaves and stands, no consensus exists about the nature of productivity at the scale of the individual tree, in part because we lack a broad empirical assessment of whether rates of absolute tree mass growth (and thus carbon accumulation) decrease, remain constant, or increase as trees increase in size and age. Here we present a global analysis of 403 tropical and temperate tree species, showing that for most species mass growth rate increases continuously with tree size. Thus, large, old trees do not act simply as senescent carbon reservoirs but actively fix large amounts of carbon compared to smaller trees; at the extreme, a single big tree can add the same amount of carbon to the forest within a year as is contained in an entire mid-sized tree. The apparent paradoxes of individual tree growth increasing with tree size despite declining leaf-level and stand-level productivity can be explained, respectively, by increases in a tree's total leaf area that outpace declines in productivity per unit of leaf area and, among other factors, age-related reductions in population density. Our results resolve conflicting assumptions about the nature of tree growth, inform efforts to undertand and model forest carbon dynamics, and have additional implications for theories of resource allocation and plant senescence. PMID:24429523

  5. From Gene to Organismal Phylogeny: Reconciled Trees and the Gene Tree/Species Tree Problem

    E-print Network

    Page, Roderic

    From Gene to Organismal Phylogeny: Reconciled Trees and the Gene Tree/Species Tree Problem Roderic The processes of gene duplication, loss, and lineage sorting can result in incongruence between the phylog- enies of genes and those of species. This incongruence complicates the task of inferring the latter from

  6. Plasma cholinesterase inhibition in the clay-colored robin (Turdus grayi) exposed to diazinon in maradol papaya crops in Yucatan, Mexico

    USGS Publications Warehouse

    Cobos, V.M.; Mora, M.A.; Escalona, G.

    2006-01-01

    The use of organophosphorous pesticides in agriculture can result in intoxication of birds foraging in sprayed crops. Effects on birds resulting from pesticide intoxication are varied and include behavioral and reproductive effects, including death. One widely used insecticide in Maradol papaya crops is diazinon which has been associated with various incidents of intoxication and death of wild birds. The objective of this study was to evaluate the impact of diazinon application to papaya crops on plasma cholinesterase activity of the clay-colored robin (Turdus grayi). We captured clay-colored robins foraging in a papaya crop the following day after the field had been sprayed with diazinon at a dose of 1.5 kg/ha during March and May, respectively. We took a blood sample from the brachialis vein of the birds captured and measured plasma enzymatic activity. The plasma samples from birds used as controls were taken during the same time period and were analyzed in a similar way. Enzymatic activity of males was greater than that of females (53,52%) and mean cholinesterase inhibition was 49.43%. Cholinesterase inhibition was greater during May than in March probably due to more continuous exposure and ingestion of the insecticide through food and possible absorption through the skin. This degree of enzymatic inhibition is possibly affecting the behavior of the clay-colored robin and could result in death in severe cases.

  7. Better Rooting Procedure to Enhance Survival Rate of Field Grown Malaysian Eksotika Papaya Transformed with 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Gene

    PubMed Central

    Sekeli, Rogayah; Abdullah, Janna Ong; Namasivayam, Parameswari; Muda, Pauziah; Abu Bakar, Umi Kalsom

    2013-01-01

    A high survival rate for transformed papaya plants when transferred to the field is useful in the quest for improving the commercial quality traits. We report in this paper an improved rooting method for the production of transformed Malaysian Eksotika papaya with high survival rate when transferred to the field. Shoots were regenerated from embryogenic calli transformed with antisense and RNAi constructs of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) genes using the Agrobacterium tumefaciens-mediated transformation method. Regenerated transformed shoots, each measuring approximately 3-4?cm in height, were cultured in liquid half-strength Murashige and Skoog (MS) medium or sterile distilled water, and with either perlite or vermiculite supplementation. All the culturing processes were conducted either under sterile or nonsterile condition. The results showed that rooting under sterile condition was better. Shoots cultured in half-strength MS medium supplemented with vermiculite exhibited a 92.5% rooting efficiency while perlite showed 77.5%. The survival rate of the vermiculite-grown transformed papaya plantlets after transfer into soil, contained in polybags, was 94%, and the rate after transfer into the ground was 92%. Morpho-histological analyses revealed that the tap roots were more compact, which might have contributed to the high survival rates of the plantlets. PMID:25969786

  8. Global Value Trees

    PubMed Central

    Zhu, Zhen; Puliga, Michelangelo; Cerina, Federica; Chessa, Alessandro; Riccaboni, Massimo

    2015-01-01

    The fragmentation of production across countries has become an important feature of the globalization in recent decades and is often conceptualized by the term “global value chains” (GVCs). When empirically investigating the GVCs, previous studies are mainly interested in knowing how global the GVCs are rather than how the GVCs look like. From a complex networks perspective, we use the World Input-Output Database (WIOD) to study the evolution of the global production system. We find that the industry-level GVCs are indeed not chain-like but are better characterized by the tree topology. Hence, we compute the global value trees (GVTs) for all the industries available in the WIOD. Moreover, we compute an industry importance measure based on the GVTs and compare it with other network centrality measures. Finally, we discuss some future applications of the GVTs. PMID:25978067

  9. Global value trees.

    PubMed

    Zhu, Zhen; Puliga, Michelangelo; Cerina, Federica; Chessa, Alessandro; Riccaboni, Massimo

    2015-01-01

    The fragmentation of production across countries has become an important feature of the globalization in recent decades and is often conceptualized by the term "global value chains" (GVCs). When empirically investigating the GVCs, previous studies are mainly interested in knowing how global the GVCs are rather than how the GVCs look like. From a complex networks perspective, we use the World Input-Output Database (WIOD) to study the evolution of the global production system. We find that the industry-level GVCs are indeed not chain-like but are better characterized by the tree topology. Hence, we compute the global value trees (GVTs) for all the industries available in the WIOD. Moreover, we compute an industry importance measure based on the GVTs and compare it with other network centrality measures. Finally, we discuss some future applications of the GVTs. PMID:25978067

  10. Insert tree completion system

    SciTech Connect

    Brands, K.W.; Ball, I.G.; Cegielski, E.J.; Gresham, J.S.; Saunders, D.N.

    1982-09-01

    This paper outlines the overall project for development and installation of a low-profile, caisson-installed subsea Christmas tree. After various design studies and laboratory and field tests of key components, a system for installation inside a 30-in. conductor was ordered in July 1978 from Cameron Iron Works Inc. The system is designed to have all critical-pressure-containing components below the mudline and, with the reduced profile (height) above seabed, provides for improved safety of satellite underwater wells from damage by anchors, trawl boards, and even icebergs. In addition to the innovative nature of the tree design, the completion includes improved 3 1/2-in. through flowline (TFL) pumpdown completion equipment with deep set safety valves and a dual detachable packer head for simplified workover capability. The all-hydraulic control system incorporates a new design of sequencing valve for both Christmas tree control and remote flowline connection. A semisubmersible drilling rig was used to initiate the first end flowline connection at the wellhead for subsequent tie-in to the prelaid, surface-towed, all-welded subsea pipeline bundle.

  11. Top-Working Pecan Trees

    E-print Network

    Kyle, E. J.

    1916-01-01

    and Mechanical College of Texas (In cooperation with the United States Department of Agriculture.) ,I' APRIL, 1916 EXTENSION SERVICE No. B-21 TOP-WORKING PECAN TREES Address CLARENCE OUSLEY Director of Extension Service, College Station, Texas TOP WORKING PECAN... TREES By E. J. Kyle, Professor of Horticulture, Dean of the School of Agriculture, Agricultural and Mechanical College of Texas. Top-working pecan trees has passed beyond the experimental stage. Some of the methods employed during the experimental period...

  12. Barking up the Right Tree

    ERIC Educational Resources Information Center

    Houston, Paul D.

    2006-01-01

    There is a childhood saying about a confused dog who thinks he sees a possum in a tree. The problem is that the possum is actually in a different tree so the dog barks up the wrong tree. American education is constantly playing both dog and possum. Sometimes they are the prey, and sometimes they are just confused about what and where the prey is.…

  13. Collapse of loaded fractal trees

    NASA Astrophysics Data System (ADS)

    Turcotte, D. L.; Smalley, R. F.; Solla, Sara A.

    1985-02-01

    Mandelbrot1 has argued that a wide range of natural objects and phenomena are fractals; examples of fractal trees include actual trees, plants such as a cauliflower, river systems and the cardiovascular system. Here we apply the renormalization group approach2 to the collapse of fractal trees, which may be applicable to a variety of problems including cardiac arrest, failure of bronchial systems, failure of electrical distribution systems and the instability resulting in earthquakes.

  14. Human decision error (HUMDEE) trees

    SciTech Connect

    Ostrom, L.T.

    1993-08-01

    Graphical presentations of human actions in incident and accident sequences have been used for many years. However, for the most part, human decision making has been underrepresented in these trees. This paper presents a method of incorporating the human decision process into graphical presentations of incident/accident sequences. This presentation is in the form of logic trees. These trees are called Human Decision Error Trees or HUMDEE for short. The primary benefit of HUMDEE trees is that they graphically illustrate what else the individuals involved in the event could have done to prevent either the initiation or continuation of the event. HUMDEE trees also present the alternate paths available at the operator decision points in the incident/accident sequence. This is different from the Technique for Human Error Rate Prediction (THERP) event trees. There are many uses of these trees. They can be used for incident/accident investigations to show what other courses of actions were available and for training operators. The trees also have a consequence component so that not only the decision can be explored, also the consequence of that decision.

  15. Street Tree Inventory Report and Recommendations

    E-print Network

    and planting. * Conduct an annual Arbor Day celebration and involve local groups. * Craft a public tree care Street Tree Care Replacement Value Street Tree Values Recommendations A. Data Collection Form B. Species% require only routine care. * An estimated 2,200 public trees and 1,900 private trees have limbs

  16. Sugar Maple Tree Photo by Matt Hajdun

    E-print Network

    Ashline, George

    Sugar Maple Tree Photo by Matt Hajdun Did you know that the Sugar Maple Tree can reach heights of 130 feet or more and live to be very old. Activity for Saplings Story of a Sugar Maple Materials. · Read Tell Me, Tree. · Collect a few Sugar Maple leaves from the tree. · Look at the tree

  17. Small integral trees A. E. Brouwer

    E-print Network

    Brouwer, Andries E.

    Small integral trees A. E. Brouwer 2007-12-31 Abstract We give a table with all integral trees on at most 50 vertices, and characterize integral trees with a single eigenvalue 0. 1 Integral trees A finite graph is called integral if the spectrum of its adjacency matrix has only integral eigenvalues. A tree

  18. COMPONENT User's Guide Maps between trees

    E-print Network

    Page, Roderic

    COMPONENT User's Guide Chapter 7 Maps between trees This chapter describes tree mapping routines in COMPONENT. These routines allow you to map gene trees onto species trees, parasite phylogenies onto host phylogenies, and taxon cladograms onto area cladograms. Overview This section introduces some basics of tree

  19. Simaroubaceae (Quassia family) Tree-of-heaven

    E-print Network

    Simaroubaceae (Quassia family) Tree-of-heaven Ailanthus altissima (Mill.) Swingle Life cycle Fast leaves emit an unpleasant odor. Glands on underside of leaflet teeth. Tree-of-heaven sapling. Tree-of-heaven Seeds and prolific vegetative sprouts. Tree-of-heaven continued Tree-of-heaven weedy habit. #12;

  20. DIF Trees: Using Classification Trees to Detect Differential Item Functioning

    ERIC Educational Resources Information Center

    Vaughn, Brandon K.; Wang, Qiu

    2010-01-01

    A nonparametric tree classification procedure is used to detect differential item functioning for items that are dichotomously scored. Classification trees are shown to be an alternative procedure to detect differential item functioning other than the use of traditional Mantel-Haenszel and logistic regression analysis. A nonparametric…

  1. Relating phylogenetic trees to transmission trees of infectious disease outbreaks.

    PubMed

    Ypma, Rolf J F; van Ballegooijen, W Marijn; Wallinga, Jacco

    2013-11-01

    Transmission events are the fundamental building blocks of the dynamics of any infectious disease. Much about the epidemiology of a disease can be learned when these individual transmission events are known or can be estimated. Such estimations are difficult and generally feasible only when detailed epidemiological data are available. The genealogy estimated from genetic sequences of sampled pathogens is another rich source of information on transmission history. Optimal inference of transmission events calls for the combination of genetic data and epidemiological data into one joint analysis. A key difficulty is that the transmission tree, which describes the transmission events between infected hosts, differs from the phylogenetic tree, which describes the ancestral relationships between pathogens sampled from these hosts. The trees differ both in timing of the internal nodes and in topology. These differences become more pronounced when a higher fraction of infected hosts is sampled. We show how the phylogenetic tree of sampled pathogens is related to the transmission tree of an outbreak of an infectious disease, by the within-host dynamics of pathogens. We provide a statistical framework to infer key epidemiological and mutational parameters by simultaneously estimating the phylogenetic tree and the transmission tree. We test the approach using simulations and illustrate its use on an outbreak of foot-and-mouth disease. The approach unifies existing methods in the emerging field of phylodynamics with transmission tree reconstruction methods that are used in infectious disease epidemiology. PMID:24037268

  2. Sussing Merger Trees: A proposed Merger Tree data format

    E-print Network

    Thomas, Peter A; Tweed, Dylan; Benson, Andrew J; Croton, Darren; Elahi, Pascal; Henriques, Bruno; Iliev, Ilian T; Knebe, Alexander; Lux, Hanni; Mao, Yao-Yuan; Neyrinck, Mark; Pearce, Frazer R; Rodriguez-Gomez, Vicente; Schneider, Aurel; Srisawat, Chaichalit

    2015-01-01

    We propose a common terminology for use in describing both temporal merger trees and spatial structure trees for dark-matter halos. We specify a unified data format in HDF5 and provide example I/O routines in C, FORTRAN and PYTHON.

  3. Red-Black Trees 1 2004 Goodrich, Tamassia Red-Black Trees

    E-print Network

    Alechina, Natasha

    Red-Black Trees 1© 2004 Goodrich, Tamassia Red-Black Trees 6 3 8 4 v z #12;Red-Black Trees 2© 2004 Goodrich, Tamassia From (2,4) to Red-Black Trees A red-black tree is a representation of a (2,4) tree by means of a binary tree whose nodes are colored red or black In comparison with its associated (2,4) tree

  4. Two Trees: Migrating Fault Trees to Decision Trees for Real Time Fault Detection on International Space Station

    NASA Technical Reports Server (NTRS)

    Lee, Charles; Alena, Richard L.; Robinson, Peter

    2004-01-01

    We started from ISS fault trees example to migrate to decision trees, presented a method to convert fault trees to decision trees. The method shows that the visualizations of root cause of fault are easier and the tree manipulating becomes more programmatic via available decision tree programs. The visualization of decision trees for the diagnostic shows a format of straight forward and easy understands. For ISS real time fault diagnostic, the status of the systems could be shown by mining the signals through the trees and see where it stops at. The other advantage to use decision trees is that the trees can learn the fault patterns and predict the future fault from the historic data. The learning is not only on the static data sets but also can be online, through accumulating the real time data sets, the decision trees can gain and store faults patterns in the trees and recognize them when they come.

  5. Distributed Merge Trees

    SciTech Connect

    Morozov, Dmitriy; Weber, Gunther

    2013-01-08

    Improved simulations and sensors are producing datasets whose increasing complexity exhausts our ability to visualize and comprehend them directly. To cope with this problem, we can detect and extract significant features in the data and use them as the basis for subsequent analysis. Topological methods are valuable in this context because they provide robust and general feature definitions. As the growth of serial computational power has stalled, data analysis is becoming increasingly dependent on massively parallel machines. To satisfy the computational demand created by complex datasets, algorithms need to effectively utilize these computer architectures. The main strength of topological methods, their emphasis on global information, turns into an obstacle during parallelization. We present two approaches to alleviate this problem. We develop a distributed representation of the merge tree that avoids computing the global tree on a single processor and lets us parallelize subsequent queries. To account for the increasing number of cores per processor, we develop a new data structure that lets us take advantage of multiple shared-memory cores to parallelize the work on a single node. Finally, we present experiments that illustrate the strengths of our approach as well as help identify future challenges.

  6. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 36 Parks, Forests, and Public Property 2 2013-07-01 2013-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of trees...

  7. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 36 Parks, Forests, and Public Property 2 2011-07-01 2011-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of trees...

  8. Non-crossing Trees are Almost Conditioned Galton-Watson trees1

    E-print Network

    Marckert, Jean-François

    Non-crossing Trees are Almost Conditioned Galton-Watson trees1 Jean-Fran¸cois Marckert Alois-10, A - 1040 Wien, Austria alois.panholzer@tuwien.ac.at ABSTRACT: A non-crossing tree (NC-tree) is a tree drawn seg- ments that do not cross. In this paper, we show that NC-trees with size n are conditioned Galton

  9. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 36 Parks, Forests, and Public Property 2 2012-07-01 2012-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of trees...

  10. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 36 Parks, Forests, and Public Property 2 2014-07-01 2014-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests, and Public Property FOREST SERVICE, DEPARTMENT OF... PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of trees...

  11. A New Pruning Method for Solving Decision Trees and Game Trees

    E-print Network

    Shenoy, Prakash P.

    1995-08-01

    The main goal of this paper is to describe a newpruning method for solving decision trees and game trees. The pruning method for decision trees suggests a slight variant of decision trees that we call scenario trees. In scenario trees, we do...

  12. Decision Trees Decision trees are a popular method for classifying objects by means

    E-print Network

    Hamburg,.Universität

    1 1 Decision Trees Decision trees are a popular method for classifying objects by means of a sequence of tests of feature values following a tree structure. Example : Decision tree for classifying A decision tree is a tree where: · the non-leaf nodes are labeled with attributes, · all arcs out of a node

  13. On the Number of Ranked Species Trees Producing Anomalous Ranked Gene Trees

    E-print Network

    Rosenberg, Noah

    On the Number of Ranked Species Trees Producing Anomalous Ranked Gene Trees Filippo Disanto demonstrated the existence of anomalous ranked gene trees (ARGTs), ranked gene trees that are more probable than the ranked gene tree that accords with the ranked species tree. Here, to improve

  14. Reconciling a Gene Tree to a Species Tree Under the Duplication Paola Bonizzoni

    E-print Network

    Della Vedova, Gianluca

    Reconciling a Gene Tree to a Species Tree Under the Duplication Cost Model Paola Bonizzoni Gianluca from evolutionary trees representing the relationships between distinct gene families is of great of minimum tree that reconciles a gene tree and a species tree is correct. We answer affirmatively

  15. Oak trees havevariedeffect on landvalues

    E-print Network

    County seat, Santa Rosa, is about 60 miles from San Francisco. The coun- ty's population of 340 of several blue oak stands with different tree density were taken in July 1986 in Briones Regional Park for five blue oak stands, Briones Regional Park, Contra Costa county Trees Basal Volume area per Crown acre

  16. The tree of one percent

    PubMed Central

    Dagan, Tal; Martin, William

    2006-01-01

    Two significant evolutionary processes are fundamentally not tree-like in nature - lateral gene transfer among prokaryotes and endosymbiotic gene transfer (from organelles) among eukaryotes. To incorporate such processes into the bigger picture of early evolution, biologists need to depart from the preconceived notion that all genomes are related by a single bifurcating tree. PMID:17081279

  17. Climate change & street trees project

    E-print Network

    Climate change & street trees project Social Research Report The social and cultural values Group as part of the Climate change and street trees project, funded by the Forestry Commission with changing socio-economics and/or demographics, but little evidence exists relating

  18. Looking/Learning Drawing: Trees.

    ERIC Educational Resources Information Center

    Hurwitz, Al; Blume, Sharon

    1985-01-01

    Secondary students are asked to study and compare three reproductions--Van Gogh's "Grove of Cypresses," Da Vinci's "Study of a Tree," and Mondrian's "Tree II." The activity will help students develop their powers of observation and analysis, powers that can be applied to their own drawings. (RM)

  19. Tree Hydraulics: How Sap Rises

    ERIC Educational Resources Information Center

    Denny, Mark

    2012-01-01

    Trees transport water from roots to crown--a height that can exceed 100 m. The physics of tree hydraulics can be conveyed with simple fluid dynamics based upon the Hagen-Poiseuille equation and Murray's law. Here the conduit structure is modelled as conical pipes and as branching pipes. The force required to lift sap is generated mostly by…

  20. 75 FR 25103 - Tree Assistance Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-05-07

    ...Corporation 7 CFR Part 1416 RIN 0560-AH96 Tree Assistance Program AGENCY: Farm Service...implements specific requirements for the Tree Assistance Program (TAP) authorized by...assistance to eligible orchardists and nursery tree growers to replant or rehabilitate...

  1. Latent Tree Classifier , Nevin L. Zhang2

    E-print Network

    Zhang, Nevin L.

    on latent tree models. A latent tree model (LTM) [23] is a tree-structured Bayesian network in which be different across classes. Therefore, we build an LTM for each class. We refer to the collection of LTMs plus

  2. Optimal Decision Trees Kristin P. Bennett \\Lambda

    E-print Network

    Mitchell, John E.

    Optimal Decision Trees Kristin P. Bennett \\Lambda Jennifer A. Blue Department of Mathematical an Extreme Point Tabu Search (EPTS) algorithm that constructs globally optimal decision trees for classification problems. Typically, decision tree algorithms are greedy. They optimize the misclassification

  3. 7 CFR 1214.3 - Christmas tree.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ...2012-01-01 2012-01-01 false Christmas tree. 1214.3 Section 1214.3 Agriculture...DEPARTMENT OF AGRICULTURE CHRISTMAS TREE PROMOTION, RESEARCH, AND INFORMATION ORDER Christmas Tree Promotion, Research, and Information...

  4. 7 CFR 1214.3 - Christmas tree.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ...2013-01-01 2013-01-01 false Christmas tree. 1214.3 Section 1214.3 Agriculture...DEPARTMENT OF AGRICULTURE CHRISTMAS TREE PROMOTION, RESEARCH, AND INFORMATION ORDER Christmas Tree Promotion, Research, and Information...

  5. 7 CFR 1214.3 - Christmas tree.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ...2014-01-01 2014-01-01 false Christmas tree. 1214.3 Section 1214.3 Agriculture...DEPARTMENT OF AGRICULTURE CHRISTMAS TREE PROMOTION, RESEARCH, AND INFORMATION ORDER Christmas Tree Promotion, Research, and Information...

  6. Quantum Computation and Decision Trees

    E-print Network

    Edward Farhi; Sam Gutmann

    1998-03-20

    Many interesting computational problems can be reformulated in terms of decision trees. A natural classical algorithm is to then run a random walk on the tree, starting at the root, to see if the tree contains a node n levels from the root. We devise a quantum mechanical algorithm that evolves a state, initially localized at the root, through the tree. We prove that if the classical strategy succeeds in reaching level n in time polynomial in n, then so does the quantum algorithm. Moreover, we find examples of trees for which the classical algorithm requires time exponential in n, but for which the quantum algorithm succeeds in polynomial time. The examples we have so far, however, could also be solved in polynomial time by different classical algorithms.

  7. A Cost Benefit Analysis of Urban Trees

    SciTech Connect

    Akbari, Hashem

    1997-09-01

    Urban shade trees offer significant benefits in reducing building air- conditioning and improving urban air quality by reducing smog. The savings associated with these benefits varies by climate regions and can be up to $200 per tree. The cost of planting trees and maintaining them can vary from $10 to $500 per tree. Tree planting programs can be designed offer savings to communities that plant trees.

  8. Tree reconstruction from partial orders

    SciTech Connect

    Kannan, S.K.; Warnow, T.J.

    1993-03-01

    The problem of constructing trees given a matrix of interleaf distances is motivated by applications in computational evolutionary biology and linguistics. The general problem is to find an edge-weighted tree which most closely approximates the distance matrix. Although the construction problem is easy when the tree exactly fits the distance matrix, optimization problems under all popular criteria are either known or conjectured to be NP-complete. In this paper we consider the related problem where we are given a partial order on the pairwise distances, and wish to construct (if possible) an edge-weighted tree realizing the partial order. In particular we are interested in partial orders which arise from experiments on triples of species, which determine either a linear ordering of the three pairwise distances (called Total Order Model or TOM experiments) or only the pair(s) of minimum distance apart (called Partial Order Model or POM experiments). The POM and TOM experimental model is inspired by the model proposed by Kannan, Lawler, and Warnow for constructing trees from experiments which determine the rooted topology for any triple of species. We examine issues of construction of trees and consistency of TOM and POM experiments, where the trees may either be weighted or unweighted. Using these experiments to construct unweighted trees without nodes of degree two is motivated by a similar problem studied by Winkler, called the Discrete Metric Realization problem, which he showed to be strongly NP-hard. We have the following results: Determining consistency of a set of TOM or POM experiments is NP-Complete whether the tree is weighted or constrained to be unweighted and without degree two nodes. We can construct unweighted trees without degree two nodes from TOM experiments in optimal O(n{sup 3}) time and from POM experiments in O(n{sup 4}) time.

  9. Tree reconstruction from partial orders

    SciTech Connect

    Kannan, S.K. ); Warnow, T.J. )

    1993-01-01

    The problem of constructing trees given a matrix of interleaf distances is motivated by applications in computational evolutionary biology and linguistics. The general problem is to find an edge-weighted tree which most closely approximates the distance matrix. Although the construction problem is easy when the tree exactly fits the distance matrix, optimization problems under all popular criteria are either known or conjectured to be NP-complete. In this paper we consider the related problem where we are given a partial order on the pairwise distances, and wish to construct (if possible) an edge-weighted tree realizing the partial order. In particular we are interested in partial orders which arise from experiments on triples of species, which determine either a linear ordering of the three pairwise distances (called Total Order Model or TOM experiments) or only the pair(s) of minimum distance apart (called Partial Order Model or POM experiments). The POM and TOM experimental model is inspired by the model proposed by Kannan, Lawler, and Warnow for constructing trees from experiments which determine the rooted topology for any triple of species. We examine issues of construction of trees and consistency of TOM and POM experiments, where the trees may either be weighted or unweighted. Using these experiments to construct unweighted trees without nodes of degree two is motivated by a similar problem studied by Winkler, called the Discrete Metric Realization problem, which he showed to be strongly NP-hard. We have the following results: Determining consistency of a set of TOM or POM experiments is NP-Complete whether the tree is weighted or constrained to be unweighted and without degree two nodes. We can construct unweighted trees without degree two nodes from TOM experiments in optimal O(n[sup 3]) time and from POM experiments in O(n[sup 4]) time.

  10. Deployment of response surface methodology to optimize recovery of dark fresh fig (Ficus carica L., var. Azenjar) total phenolic compounds and antioxidant activity.

    PubMed

    Bachir Bey, Mostapha; Meziant, Leila; Benchikh, Yassine; Louaileche, Hayette

    2014-11-01

    Optimum conditions for extracting total phenolic compounds (TPC) and antioxidant activity from fresh dark fig (Ficus carica L.) have been investigated using response surface methodology (RSM). The Box-Behnken design was used to investigate the effects of three independent variables, acetone concentration (40-80%), temperature (25-65 °C), and time (60-120 min), on the response. Regression analysis showed that about 96% of the variation was explained by the models. P-value for the lack of fit was insignificant which confirmed the validity of models. Response surface analysis showed that the optimal extraction parameters that maximized antioxidants extraction were 63.48% acetone, 115.14 min, and 48.66 °C. Under optimum conditions the corresponding experimental values for TPC and antioxidant activity were 536.43 and 71.86 mg GAE/100 g DM. The experimental values are in accordance with those predicted, indicating the suitability of the model and the success of RSM in optimizing the extraction conditions. PMID:24874388

  11. In vitro antioxidant, collagenase inhibition, and in vivo anti-wrinkle effects of combined formulation containing Punica granatum, Ginkgo biloba, Ficus carica, and Morus alba fruits extract

    PubMed Central

    Ghimeray, Amal Kumar; Jung, Un Sun; Lee, Ha Youn; Kim, Young Hoon; Ryu, Eun Kyung; Chang, Moon Sik

    2015-01-01

    Background In phytotherapy, the therapeutic potential is based on the combined action of different herbal drugs. Our objective was to evaluate the antioxidant, anti-collagenase (in vitro), and anti-wrinkle (in vivo) effect of combined formulation containing Ginkgo biloba, Punica granatum, Ficus carica, and Morus alba fruits extract. Methods Antioxidant evaluation was based on the scavenging activity of free radicals (1,1-diphenyl-2-picrylhydrazyl, H2O2, and O2?) and the anti-collagenase activity was based on the reduction of collagenase enzyme in vitro. In an in vivo study, 21 female subjects were examined in a placebo-controlled trail. Facial wrinkle, especially the crow’s feet region of eyes, was treated with topical formulated 2% cream for 56 days and compared with the placebo. Results In the in vitro study, the combination of fruits extract showed a higher antioxidant activity which was comparable with the positive standard (ascorbic acid, butylated hydroxyanisole, and Trolox). The data also showed a dose-dependent inhibition of collagenase. In the in vivo study, treatment with 2% formulated cream for 56 days significantly reduced the percentage of wrinkle depth, length, and area with 11.5, 10.07, and 29.55, respectively. Conclusion The combined formulation of fruit extracts showed excellent antioxidative and anti-collagenase activity as well as a significant effect on anti-wrinkle activity on human skin. PMID:26203268

  12. Gas chromatography/mass spectrometry and pyrolysis-gas chromatography/mass spectrometry for the chemical characterisation of modern and archaeological figs (Ficus carica).

    PubMed

    Ribechini, Erika; Pérez-Arantegui, Josefina; Colombini, Maria Perla

    2011-06-24

    Gas chromatography/mass spectrometry (GC/MS) after alkaline hydrolysis, solvent extraction and trimethylsilylation, and analytical pyrolysis using hexamethyldisilazane (HMDS) for in situ derivatisation followed by gas chromatographic/mass spectrometric analysis (Pyrolysis-silylation-GC/MS) were used to investigate the hydrolysable and soluble constituents, and the polymerised macromolecules of an archaeological fig (Ficus carica) recovered in Zaragoza (Spain), as well as of modern figs. The main aim was to study the compositional alterations undergone by the fig tissues in a particular archaeological environment: the fig was in a vessel and covered by a layer of a mixture of orpiment and gypsum. A comparison between the GC/MS results from modern and archaeological figs revealed that degradative reactions took place, leading to the disappearance/depletion of reactive (unsaturated fatty acids) and sensitive compounds (phytosterols and triterpenes). Py-silylation-GC/MS data provided evidence of a significant degradation of the saccharide and lipid components of the fig tissue, which left a residue enriched in polyphenols and polyesters. PMID:21570079

  13. 77 FR 25903 - Thiamethoxam; Pesticide Tolerances; Technical Correction

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-05-02

    ...caneberry subgroup 13-07A; mustard, seed; onion, dry bulb; papaya; safflower, seed; and nut, tree, group 14. DATES: This...13-07A; mustard, seed; onion, dry bulb; papaya; safflower, seed; and nut, tree, group 14 to the...

  14. Terrestrial apes and phylogenetic trees

    PubMed Central

    Arsuaga, Juan Luis

    2010-01-01

    The image that best expresses Darwin’s thinking is the tree of life. However, Darwin’s human evolutionary tree lacked almost everything because only the Neanderthals were known at the time and they were considered one extreme expression of our own species. Darwin believed that the root of the human tree was very deep and in Africa. It was not until 1962 that the root was shown to be much more recent in time and definitively in Africa. On the other hand, some neo-Darwinians believed that our family tree was not a tree, because there were no branches, but, rather, a straight stem. The recent years have witnessed spectacular discoveries in Africa that take us close to the origin of the human tree and in Spain at Atapuerca that help us better understand the origin of the Neanderthals as well as our own species. The final form of the tree, and the number of branches, remains an object of passionate debate. PMID:20445090

  15. Through bore subsea christmas trees

    SciTech Connect

    Huber, D.S.; Simmers, G.F.C.; Johnson, C.S.

    1985-01-01

    The workovers of subsea completed wells are expensive and time consuming as even the most routine tasks must be carried out by a semi-submersible. This paper describes the economic, safety and operational advantages which led to the development and successful first installation of 'through bore' subsea production trees. The conventional wet subsea trees have proved to be very reliable over the past ten years of operation in the Argyll, Duncan and Innes fields, however the completion strings require pulling on the average about once every three to five years. The conventional subsea tree/tubing hanger set up design requires the tree to be tripped and a rig BOP stack run to pull the tubing. This operation is time consuming, very weather sensitive and leaves the well temporarily without a well control stack on the wellhead. The 7 1/16'' 'through bore' subsea tree was developed to minimize the tubing pulling workover time and several trees have been run successfully since the latter part of 1984. The time saving on a tubing pulling workover is three days. In addition, the design considerably reduces the hazards and equipment damage risk inherent in the conventional design. Hamilton Brothers and National Supply Company in Aberdeen designed the equipment which must be considered a new generation of subsea production trees.

  16. Tree-growth analyses to estimate tree species' drought tolerance.

    PubMed

    Eilmann, Britta; Rigling, Andreas

    2012-02-01

    Climate change is challenging forestry management and practices. Among other things, tree species with the ability to cope with more extreme climate conditions have to be identified. However, while environmental factors may severely limit tree growth or even cause tree death, assessing a tree species' potential for surviving future aggravated environmental conditions is rather demanding. The aim of this study was to find a tree-ring-based method suitable for identifying very drought-tolerant species, particularly potential substitute species for Scots pine (Pinus sylvestris L.) in Valais. In this inner-Alpine valley, Scots pine used to be the dominating species for dry forests, but today it suffers from high drought-induced mortality. We investigate the growth response of two native tree species, Scots pine and European larch (Larix decidua Mill.), and two non-native species, black pine (Pinus nigra Arnold) and Douglas fir (Pseudotsuga menziesii Mirb. var. menziesii), to drought. This involved analysing how the radial increment of these species responded to increasing water shortage (abandonment of irrigation) and to increasingly frequent drought years. Black pine and Douglas fir are able to cope with drought better than Scots pine and larch, as they show relatively high radial growth even after irrigation has been stopped and a plastic growth response to drought years. European larch does not seem to be able to cope with these dry conditions as it lacks the ability to recover from drought years. The analysis of trees' short-term response to extreme climate events seems to be the most promising and suitable method for detecting how tolerant a tree species is towards drought. However, combining all the methods used in this study provides a complete picture of how water shortage could limit species. PMID:22363071

  17. Falls from trees and tree associated injuries in rural Melanesians.

    PubMed

    Barss, P; Dakulala, P; Doolan, M

    Falls from trees and other tree related injuries are the most common cause of trauma in some parts of rural Melanesia. A four year review of all admissions for trauma to the Provincial Hospital at Alotau, Milne Bay Province, Papua New Guinea, showed that 27% were due to falls from trees, and a further 10% were due to related injuries, such as being struck by a falling branch or a coconut. A questionnaire distributed to rural health centres showed that during the study period at least 28 villagers died from falls from trees before reaching hospital. Head and chest trauma were common causes of death. Many injured patients were boys. Forearm fractures were the most common injuries, but more serious injuries were also frequently encountered. Trees responsible for most deaths and injuries included the coconut palm, betel palm, mango, and breadfruit. There are many strategies for preventing such injuries; perhaps the most important is to stop small boys climbing tall trees. Such falls are a serious occupational hazard for many subsistence farmers. PMID:6440619

  18. Implementing Municipal Tree Planting: Los Angeles Million-Tree Initiative

    NASA Astrophysics Data System (ADS)

    Pincetl, Stephanie

    2010-02-01

    Urban forests are increasingly being seen as an important infrastructure that can help cities remediate their environmental impacts. This work reports on the first steps in implementing a million tree program in Los Angeles and the ways such a biogenic—living—infrastructure has been approached. Numbers of studies have been done to quantify the benefits of urban forests, but little has been written on the process of implementing urban tree planting programs. The investigative methods were primarily qualitative, involving interviews, attending meetings and conducting literature reviews. Results indicate that multiple nonprofit and city agency programs are involved in planting and maintaining trees and this has required coordination among groups that here-to-fore were unaccustomed to having to collaborate. The main finding that emerge from this research is that the implementation of such a program in Los Angeles is more complicated than it may seem due to several interacting factors: the need to rely on multiple public and private organizations to put trees into the ground and to maintain them; coordination of these multiple efforts must be centralized, but requires a great deal of time and effort and maybe resisted by some of the partners; funding for planting and long term maintenance must be pieced together from multiple sources; acceptance of trees by residents varies by neighborhood as does tree canopy cover; appropriate nursery supply can be limited; the location of the program within the city administration is determined by who initiates the program.

  19. Assessment of Prey Preference by the Generalist Predator, Mallada basalis (Walker), When Offered Two Species of Spider Mites, Tetranychus kanzawai Kishida and Panonychus citri (McGregor) on Papaya

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We investigated potential prey preference of the generalist predator Mallada basalis (Walker) when offered two mites, Tetranychus kanzawai Kishida and Panonychus citri (McGregor), both important pests on papaya. Laboratory choice tests revealed that none of the three larval instars of M. basalis sho...

  20. Learning Latent Tree Graphical Models

    E-print Network

    Choi, Myung Jin

    We study the problem of learning a latent tree graphical model where samples are available only from a subset of variables. We propose two consistent and computationally efficient algorithms for learning minimal latent ...

  1. Sugar Maple Tree Photo by Matt Hajdun

    E-print Network

    Ashline, George

    Sugar Maple Tree Photo by Matt Hajdun Did you know the Sugar Maple tree is Vermont's State tree? Sugar Maples have a "U" shaped sinus, and the neighboring tree, the Norway Maple, has a V shaped one, there is white residue on the stem and not on the Sugar Maple. Activity for Seedlings Bark Rubbing Poem Materials

  2. How To Select and Plant a Tree.

    ERIC Educational Resources Information Center

    Fazio, James R., Ed.

    1991-01-01

    This bulletin furnishes information about selecting and planting trees. The tree selection process includes being aware of the physical characteristics of bare root seedlings, containerized seedlings, balled and burlapped, or potted trees and determining the proper size and root ball proportions. The section on tree planting discusses how to: (1)…

  3. Tunnel of the California Tunnel Tree

    USGS Multimedia Gallery

    In this image, the tunnel through the California Tunnel Tree may be seen. The tunnel was cut through the giant sequoia tree in 1895 to allow coaches, and eventually cars, to drive through the tree as part of a marketing stunt. The California Tunnel Tree is the only living giant sequoia with a t...

  4. Learning the Trees By Howard Nemerov

    E-print Network

    1 Poem Links Learning the Trees By Howard Nemerov http://www.poetryfoundation.org/poetrymagazine/poem/22392 Trees By Joyce Kilmer http://www.poetryfoundation.org/poetrymagazine/poem/1947 Native Trees By W. S. Merwin http://www.poetryfoundation.org/poem/171876 Winter Trees By William Carlos Williams http

  5. A Novel Approach for Compressing Phylogenetic Trees

    E-print Network

    Williams, Tiffani

    A Novel Approach for Compressing Phylogenetic Trees Suzanne J. Matthews, Seung-Jin Sul, and Tiffani,sulsj,tlw}@cse.tamu.edu Abstract. Phylogenetic trees are tree structures that depict relation- ships between organisms. Popular analysis techniques often produce large collections of candidate trees, which are expensive to store. We

  6. Decision Tree Learning Goals for the lecture

    E-print Network

    Page Jr., C. David

    Decision Tree Learning #12;Goals for the lecture you should understand the following concepts · the decision tree representation · the standard top-down approach to learning a tree · Occam's razor · entropy and information gain · types of decision-tree splits · test sets and unbiased estimates of accuracy · overfitting

  7. Boolean Decision trees Problems and Results,

    E-print Network

    Saks, Michael

    Boolean Decision trees Problems and Results, Old and New Michael Saks, Rutgers University #12;· Deterministic Decision Trees · Randomized Decision Trees (RDTs) and a new lower bound · Proof of lower bound ­ Influences of boolean functions ­ Influences­Decision Tree connection theorem ­ Deducing the lower bound

  8. Quantum Simulation of Phylogenetic Trees

    E-print Network

    Demosthenes Ellinas; Peter Jarvis

    2011-05-09

    Quantum simulations constructing probability tensors of biological multi-taxa in phylogenetic trees are proposed, in terms of positive trace preserving maps, describing evolving systems of quantum walks with multiple walkers. Basic phylogenetic models applying on trees of various topologies are simulated following appropriate decoherent quantum circuits. Quantum simulations of statistical inference for aligned sequences of biological characters are provided in terms of a quantum pruning map operating on likelihood operator observables, utilizing state-observable duality and measurement theory.

  9. Millersville University's Trees of Distinction

    E-print Network

    Hardy, Christopher R.

    -04-17 #12;#3: Sycamore, American plane (Platanus occidentalis) 2014-06-03 #12;#4: Male Ginkgo, Maidenhair tree (Ginkgo biloba) 2014-05-08 #12;#5: Balsam fir (Abies balsamea) 2014-04-24 #12;#6: Southern (Juglans regia) 2014-05-22 #12;#18: Female Ginkgo, Maidenhair tree (Ginkgo biloba) 2014-06-14 #12;#19: Dawn

  10. Fault Tree Analysis: A Bibliography

    NASA Technical Reports Server (NTRS)

    2000-01-01

    Fault tree analysis is a top-down approach to the identification of process hazards. It is as one of the best methods for systematically identifying an graphically displaying the many ways some things can go wrong. This bibliography references 266 documents in the NASA STI Database that contain the major concepts. fault tree analysis, risk an probability theory, in the basic index or major subject terms. An abstract is included with most citations, followed by the applicable subject terms.

  11. Improvements to a Class of Distance Matrix Methods for Inferring Species Trees from Gene Trees

    E-print Network

    Rosenberg, Noah

    Improvements to a Class of Distance Matrix Methods for Inferring Species Trees from Gene Trees available for inferring species trees from gene trees, the GLASS method of Mossel and Roch (2010 branch length estimates. Further, GLASS and STEAC have been shown to be consistent estimators of tree

  12. High-Dimensional Structure Learning of Graphical Models: Trees, Latent Trees & Beyond

    E-print Network

    Anandkumar, Animashree

    High-Dimensional Structure Learning of Graphical Models: Trees, Latent Trees & Beyond Anima Tan, and Alan Willsky. UIUC Seminar Anima Anandkumar (UCI) Trees, Latent Trees & Beyond 11/08/2010 1 / 52 #12;Graphical Models: Motivation Example: Contextual Object Recognition SKY ROAD AREA TREE CAR CAR

  13. A SIMPLE BIJECTION BETWEEN A SUBCLASS OF 2-BINARY TREES AND TERNARY TREES

    E-print Network

    Wagner, Stephan

    NOTE A SIMPLE BIJECTION BETWEEN A SUBCLASS OF 2-BINARY TREES AND TERNARY TREES HELMUT PRODINGER We consider the subclass of 2-binary trees, where · nodes are labelled black or white, · the root is labelled] that these trees are in bijection with ternary trees (and henceforth enumerated by 1 2n+1 3n n ). In this note we

  14. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ...2013-07-01 2013-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests...PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of...

  15. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ...2014-07-01 2014-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests...PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of...

  16. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ...2011-07-01 2011-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests...PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of...

  17. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ...2012-07-01 2012-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests...PRODUCTS General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions of...

  18. 36 CFR 223.4 - Exchange of trees or portions of trees.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ...2010-07-01 2010-07-01 false Exchange of trees or portions of trees. 223.4 Section 223.4 Parks, Forests...TIMBER General Provisions § 223.4 Exchange of trees or portions of trees. Trees or portions...

  19. Generic physical protection logic trees

    SciTech Connect

    Paulus, W.K.

    1981-10-01

    Generic physical protection logic trees, designed for application to nuclear facilities and materials, are presented together with a method of qualitative evaluation of the trees for design and analysis of physical protection systems. One or more defense zones are defined where adversaries interact with the physical protection system. Logic trees that are needed to describe the possible scenarios within a defense zone are selected. Elements of a postulated or existing physical protection system are tagged to the primary events of the logic tree. The likelihood of adversary success in overcoming these elements is evaluated on a binary, yes/no basis. The effect of these evaluations is propagated through the logic of each tree to determine whether the adversary is likely to accomplish the end event of the tree. The physical protection system must be highly likely to overcome the adversary before he accomplishes his objective. The evaluation must be conducted for all significant states of the site. Deficiencies uncovered become inputs to redesign and further analysis, closing the loop on the design/analysis cycle.

  20. Microwave sensing of tree trunks

    NASA Astrophysics Data System (ADS)

    Jezova, Jana; Mertens, Laurence; Lambot, Sebastien

    2015-04-01

    The main subject of this research is the observation of the inner part of living tree trunks using ground-penetrating radar (GPR). Trees are everyday part of human life and therefore it is important to pay attention to the tree conditions. The most obvious consequence of the poor tree condition is dead or injury caused by falling tree. The trunk internal structure is divided into three main parts: heartwood, sapwood and bark, which make this medium highly anisotropic and heterogeneous. Furthermore, the properties of the wood are not only specie-dependent but also depend on genetic and on environmental conditions. In urban areas the main problem for the stability of the trees relies in the apparition of decays provoked by fungi, insect or birds. This results in cavities or decreasing of the support capacity of the tree. GPR has proved itself to be a very powerful electromagnetic tool for non-destructive detection of buried objects. Since the beginning of the 20th century it has been used in several different areas (archaeology, landmine detection, civil engineering, ...). GPR uses the principle of the scattering of the electromagnetic waves that are radiated from a transmitting antenna. Then the waves propagate through the medium and are reflected from the object and then they are received by a receiving antenna. The velocity of the scattered signal is determined primarily by the permittivity of the material. The optimal functionality of the GPR was investigated using the numerical simulation tool gprMax2D. This tool is based on a Finite-Difference Time-Domain (FDTD) numerical model. Subsequently, the GPR functionality was tested using the laboratory model of a decayed tree trunk. Afterwards, the results and lessons learnt in the simplified tests will be used in the processing of the real data and will help to achieve deeper understanding of them. The laboratory model of the tree trunk was made by plastic or carton pipes and filled by sand. Space inside the model was divided into three sections to separate parts with different moisture (heartwood and sapwood) or empty space (decays). For easier manipulation with the antenna we developed a special ruler for measuring the distance along the scans. Instead of the surveying wheel we read the distance with a camera, which was fixed on the antenna and focused on the ruler with a binary pattern. Hence, during whole measurement and the data processing we were able to identify an accurate position on the tree in view of the scan. Some preliminary measurements on the trees were also conducted. They were performed using a GSSI 900 MHz antenna. Several tree species (beech, horse-chestnut, birch, ...) in Louvain-la-Neuve and Brussels, Belgium, have been investigated to see the internal structure of the tree decays. The measurements were carried out mainly by circumferential measurement around the trunk and also by vertical measurement along the trunk for approximate detection of the cavity. The comparison between the numerical simulations, simplified tree trunk model and real data from trees is presented. This research is funded by the Fonds de la Recherche Scientifique (FNRS, Belgium) and benefits from networking activities carried out within the EU COST Action TU1208 "Civil Engineering Applications of Ground Penetrating Radar".

  1. Parallel TreeSPH

    NASA Astrophysics Data System (ADS)

    Davé, Romeel; Dubinski, John; Hernquist, Lars

    1997-08-01

    We describe PTreeSPH, a gravity treecode combined with an SPH hydrodynamics code designed for parallel supercomputers having distributed memory. Our computational algorithm is based on the popular TreeSPH code of Hernquist & Katz (1989)[ApJS, 70, 419]. PTreeSPH utilizes a domain decomposition procedure and a synchronous hypercube communication paradigm to build self-contained subvolumes of the simulation on each processor at every timestep. Computations then proceed in a manner analogous to a serial code. We use the Message Passing Interface (MPI) communications package, making our code easily portable to a variety of parallel systems. PTreeSPH uses individual smoothing lengths and timesteps, with a communication algorithm designed to minimize exchange of information while still providing all information required to accurately perform SPH computations. We have incorporated periodic boundary conditions with forces calculated using a quadrupole Ewald summation method, and comoving integration under a variety of cosmologies. Following algorithms presented in Katz et al. (1996)[ApJS, 105, 19], we have also included radiative cooling, heating from a parameterized ionizing background, and star formation. A cosmological simulation from z = 49 to z = 2 with 64 3 gas particles and 64 3 dark matter particles requires ˜ 1800 node-hours on a Cray T3D, with a communications overhead of ˜ 8%, load balanced to ? 95% level. When used on the new Cray T3E, this code will be capable of performing cosmological hydrodynamical simulations down to z = 0 with ˜ 2 × 10 6 particles, or to z = 2 with ˜ 10 7 particles, in a reasonable amount of time. Even larger simulations will be practical in situations where the matter is not highly clustered or when periodic boundaries are not required.

  2. Short Tree, Long Tree, Right Tree, Wrong Tree: New Acquisition Bias Corrections for Inferring SNP Phylogenies

    PubMed Central

    Leaché, Adam D.; Banbury, Barbara L.; Felsenstein, Joseph; de Oca, Adrián nieto-Montes; Stamatakis, Alexandros

    2015-01-01

    Single nucleotide polymorphisms (SNPs) are useful markers for phylogenetic studies owing in part to their ubiquity throughout the genome and ease of collection. Restriction site associated DNA sequencing (RADseq) methods are becoming increasingly popular for SNP data collection, but an assessment of the best practises for using these data in phylogenetics is lacking. We use computer simulations, and new double digest RADseq (ddRADseq) data for the lizard family Phrynosomatidae, to investigate the accuracy of RAD loci for phylogenetic inference. We compare the two primary ways RAD loci are used during phylogenetic analysis, including the analysis of full sequences (i.e., SNPs together with invariant sites), or the analysis of SNPs on their own after excluding invariant sites. We find that using full sequences rather than just SNPs is preferable from the perspectives of branch length and topological accuracy, but not of computational time. We introduce two new acquisition bias corrections for dealing with alignments composed exclusively of SNPs, a conditional likelihood method and a reconstituted DNA approach. The conditional likelihood method conditions on the presence of variable characters only (the number of invariant sites that are unsampled but known to exist is not considered), while the reconstituted DNA approach requires the user to specify the exact number of unsampled invariant sites prior to the analysis. Under simulation, branch length biases increase with the amount of missing data for both acquisition bias correction methods, but branch length accuracy is much improved in the reconstituted DNA approach compared to the conditional likelihood approach. Phylogenetic analyses of the empirical data using concatenation or a coalescent-based species tree approach provide strong support for many of the accepted relationships among phrynosomatid lizards, suggesting that RAD loci contain useful phylogenetic signal across a range of divergence times despite the presence of missing data. Phylogenetic analysis of RAD loci requires careful attention to model assumptions, especially if downstream analyses depend on branch lengths. PMID:26227865

  3. Selective and reversible thiol-pegylation, an effective approach for purification and characterization of five fully active ficin (iso)forms from Ficus carica latex.

    PubMed

    Azarkan, Mohamed; Matagne, André; Wattiez, Ruddy; Bolle, Laetitia; Vandenameele, Julie; Baeyens-Volant, Danielle

    2011-10-01

    The latex of Ficus carica constitutes an important source of many proteolytic components known under the general term of ficin (EC 3.4.22.3) which belongs to the cysteine proteases of the papain family. So far, no data on the purification and characterization of individual forms of these proteases are available. An effective strategy was used to fractionate and purify to homogeneity five ficin forms, designated A, B, C, D1 and D2 according to their sequence of elution from a cation-exchange chromatographic support. Following rapid fractionation on a SP-Sepharose Fast Flow column, the different ficin forms were chemically modified by a specific and reversible monomethoxypolyethylene glycol (mPEG) reagent. In comparison with their un-derivatized counterparts, the mPEG-protein derivatives behaved differently on the ion-exchanger, allowing us for the first time to obtain five highly purified ficin molecular species titrating 1mol of thiol group per mole of enzyme. The purified ficins were characterized by de novo peptide sequencing and peptide mass fingerprinting analyzes, using mass spectrometry. Circular dichroism measurements indicated that all five ficins were highly structured, both in term of secondary and tertiary structure. Furthermore, analysis of far-UV CD spectra allowed calculation of their secondary structural content. Both these data and the molecular masses determined by MS reinforce the view that the enzymes belong to the family of papain-like proteases. The five ficin forms also displayed different specific amidase activities against small synthetic substrates like dl-BAPNA and Boc-Ala-Ala-Gly-pNA, suggesting some differences in their active site organization. Enzymatic activity of the five ficin forms was completely inhibited by specific cysteine and cysteine/serine proteases inhibitors but was unaffected by specific serine, aspartic and metallo proteases inhibitors. PMID:21665232

  4. A Model In Which There Are Jech Kunen Trees But There Are No Kurepa Trees 1

    E-print Network

    Jin, Renling

    A Model In Which There Are Jech Kunen Trees But There Are No Kurepa Trees 1 Saharon Shelah2 and Renling Jin Abstract By an !1 tree we mean a tree of power !1 and height !1. We call an !1 tree a Jech Kunen tree if it has many branches for some strictly between !1 and 2!1 . In this paper we construct

  5. Mapping tree density at a global scale.

    PubMed

    Crowther, T W; Glick, H B; Covey, K R; Bettigole, C; Maynard, D S; Thomas, S M; Smith, J R; Hintler, G; Duguid, M C; Amatulli, G; Tuanmu, M-N; Jetz, W; Salas, C; Stam, C; Piotto, D; Tavani, R; Green, S; Bruce, G; Williams, S J; Wiser, S K; Huber, M O; Hengeveld, G M; Nabuurs, G-J; Tikhonova, E; Borchardt, P; Li, C-F; Powrie, L W; Fischer, M; Hemp, A; Homeier, J; Cho, P; Vibrans, A C; Umunay, P M; Piao, S L; Rowe, C W; Ashton, M S; Crane, P R; Bradford, M A

    2015-09-10

    The global extent and distribution of forest trees is central to our understanding of the terrestrial biosphere. We provide the first spatially continuous map of forest tree density at a global scale. This map reveals that the global number of trees is approximately 3.04 trillion, an order of magnitude higher than the previous estimate. Of these trees, approximately 1.39 trillion exist in tropical and subtropical forests, with 0.74 trillion in boreal regions and 0.61 trillion in temperate regions. Biome-level trends in tree density demonstrate the importance of climate and topography in controlling local tree densities at finer scales, as well as the overwhelming effect of humans across most of the world. Based on our projected tree densities, we estimate that over 15 billion trees are cut down each year, and the global number of trees has fallen by approximately 46% since the start of human civilization. PMID:26331545

  6. Mapping tree density at a global scale

    NASA Astrophysics Data System (ADS)

    Crowther, T. W.; Glick, H. B.; Covey, K. R.; Bettigole, C.; Maynard, D. S.; Thomas, S. M.; Smith, J. R.; Hintler, G.; Duguid, M. C.; Amatulli, G.; Tuanmu, M.-N.; Jetz, W.; Salas, C.; Stam, C.; Piotto, D.; Tavani, R.; Green, S.; Bruce, G.; Williams, S. J.; Wiser, S. K.; Huber, M. O.; Hengeveld, G. M.; Nabuurs, G.-J.; Tikhonova, E.; Borchardt, P.; Li, C.-F.; Powrie, L. W.; Fischer, M.; Hemp, A.; Homeier, J.; Cho, P.; Vibrans, A. C.; Umunay, P. M.; Piao, S. L.; Rowe, C. W.; Ashton, M. S.; Crane, P. R.; Bradford, M. A.

    2015-09-01

    The global extent and distribution of forest trees is central to our understanding of the terrestrial biosphere. We provide the first spatially continuous map of forest tree density at a global scale. This map reveals that the global number of trees is approximately 3.04 trillion, an order of magnitude higher than the previous estimate. Of these trees, approximately 1.39 trillion exist in tropical and subtropical forests, with 0.74 trillion in boreal regions and 0.61 trillion in temperate regions. Biome-level trends in tree density demonstrate the importance of climate and topography in controlling local tree densities at finer scales, as well as the overwhelming effect of humans across most of the world. Based on our projected tree densities, we estimate that over 15 billion trees are cut down each year, and the global number of trees has fallen by approximately 46% since the start of human civilization.

  7. Coalescent Histories for Lodgepole Species Trees.

    PubMed

    Disanto, Filippo; Rosenberg, Noah A

    2015-10-01

    Coalescent histories are combinatorial structures that describe for a given gene tree and species tree the possible lists of branches of the species tree on which the gene tree coalescences take place. Properties of the number of coalescent histories for gene trees and species trees affect a variety of probabilistic calculations in mathematical phylogenetics. Exact and asymptotic evaluations of the number of coalescent histories, however, are known only in a limited number of cases. Here we introduce a particular family of species trees, the lodgepole species trees (?n)n ? 0, in which tree ?n has m = 2n+1 taxa. We determine the number of coalescent histories for the lodgepole species trees, in the case that the gene tree matches the species tree, showing that this number grows with m!! in the number of taxa m. This computation demonstrates the existence of tree families in which the growth in the number of coalescent histories is faster than exponential. Further, it provides a substantial improvement on the lower bound for the ratio of the largest number of matching coalescent histories to the smallest number of matching coalescent histories for trees with m taxa, increasing a previous bound of [Formula: see text] to [Formula: see text]. We discuss the implications of our enumerative results for phylogenetic computations. PMID:25973633

  8. New program controls tree management

    SciTech Connect

    Beaty, W.

    1995-02-01

    Senior management of TransAlta Utilities Corp. (TAU) Calgary, Alberta, Canada, was highly dissatisfied with the fact that even though the distribution line clearance budget had been increased an average 35 percent a year between 1978 and 1984, there were few if any discernible positive results. Tree-related power outages kept increasing and budget requests from the field kept increasing. In searching for a solution TAU had to deal with the concept that the right level of funding can only be determined through an inventory of tree work. This inventory is comprised of two factors, the number of trees in proximity to the power lines and the local growth rates. Based on the inventory of a hired consultant, a 12-year budget projection was established. The period covered entailed a six-year, first-cycle or catch up phase, and a six-year, second-cycle maintenance phase. In implementing the new vegetation management program in 1986, TAU decided to contact each landowner directly to obtain consent to undertake the tree work. The intent was to reduce the risk of claims while maximizing tree removals. Complaints and claims were dramatically reduced and currently run at about one per 1,000 landowners and budgets have dropped back to the 1985 levels as predicted for the maintenance phase.

  9. TREE TRAILS Trees are living organisms with many specialized structures leaves, roots, wood, and the living cells

    E-print Network

    1 TREE TRAILS Trees are living organisms with many specialized structures ­ leaves, roots, wood, and the living cells that connect them. Understanding how trees are constructed and grow is essential to care for trees and calculate the benefits that trees provide. Tree Trails Tree Structure and Function Goal

  10. Measurement of tree canopy architecture

    NASA Technical Reports Server (NTRS)

    Martens, S. N.; Ustin, S. L.; Norman, J. M.

    1991-01-01

    The lack of accurate extensive geometric data on tree canopies has retarded development and validation of radiative transfer models. A stratified sampling method was devised to measure the three-dimensional geometry of 16 walnut trees which had received irrigation treatments of either 100 or 33 per cent of evapotranspirational (ET) demand for the previous two years. Graphic reconstructions of the three-dimensional geometry were verified by 58 independent measurements. The distributions of stem- and leaf-size classes, lengths, and angle classes were determined and used to calculate leaf area index (LAI), stem area, and biomass. Reduced irrigation trees have lower biomass of stems, leaves and fruit, lower LAI, steeper leaf angles and altered biomass allocation to large stems. These data can be used in ecological models that link canopy processes with remotely sensed measurements.

  11. Attention trees and semantic paths

    NASA Astrophysics Data System (ADS)

    Giusti, Christian; Pieroni, Goffredo G.; Pieroni, Laura

    2007-02-01

    In the last few decades several techniques for image content extraction, often based on segmentation, have been proposed. It has been suggested that under the assumption of very general image content, segmentation becomes unstable and classification becomes unreliable. According to recent psychological theories, certain image regions attract the attention of human observers more than others and, generally, the image main meaning appears concentrated in those regions. Initially, regions attracting our attention are perceived as a whole and hypotheses on their content are formulated; successively the components of those regions are carefully analyzed and a more precise interpretation is reached. It is interesting to observe that an image decomposition process performed according to these psychological visual attention theories might present advantages with respect to a traditional segmentation approach. In this paper we propose an automatic procedure generating image decomposition based on the detection of visual attention regions. A new clustering algorithm taking advantage of the Delaunay- Voronoi diagrams for achieving the decomposition target is proposed. By applying that algorithm recursively, starting from the whole image, a transformation of the image into a tree of related meaningful regions is obtained (Attention Tree). Successively, a semantic interpretation of the leaf nodes is carried out by using a structure of Neural Networks (Neural Tree) assisted by a knowledge base (Ontology Net). Starting from leaf nodes, paths toward the root node across the Attention Tree are attempted. The task of the path consists in relating the semantics of each child-parent node pair and, consequently, in merging the corresponding image regions. The relationship detected in this way between two tree nodes generates, as a result, the extension of the interpreted image area through each step of the path. The construction of several Attention Trees has been performed and partial results will be shown.

  12. Transport of Methane in Trees

    NASA Astrophysics Data System (ADS)

    Kutschera, E.; Khalil, A. K.; Shearer, M. J.; Rosenstiel, T.; Rice, A. L.

    2011-12-01

    Although overall methane (CH4) emissions for croplands, wetlands, and forests have been measured, the exact dynamics of CH4 transport through trees is not well understood. What roles transport mechanisms play in emission rates has been thoroughly investigated for rice, but is fairly unknown for trees. Better defined plant transport mechanisms yield more accurate determination of greenhouse gas flux and its variations, contributing to a comprehensive theory quantifying greenhouse gas emissions globally. CH4 emissions from the common wetland tree species black cottonwood (Populus trichocarpa) native to the Pacific Northwest have been measured under hydroponic conditions in order to separate plant transport processes from the influence of soil processes. Canopy emissions of CH4 have been measured via canopy enclosure. Measurements of CH4 flux from each of 16 trees have indicated that emissions are normally constant over the half-hour sampling period. Samples for stable carbon isotope composition have been taken during these experiments and measured on a mass spectrometer. Compared to the isotopic composition of root water CH4, canopy CH4 is depleted in 13C; this indicates that CH4 moving through the tree is not following a bulk flow pathway (where no depletion would occur), but instead moves either diffusively or through other cell or tissue barriers. No correlation was found to exist between leaf area and CH4 emission; this is vital to upscaling tree-level emissions to the global scale since leaf area index (LAI) cannot be treated as an appropriate parameter to upscale flux. Correctly informing global-scale CH4 fluxes from plants requires an association between the role plant physiology plays in the production and transport of CH4 and magnitudes of flux. This research was supported by the Office of Science (BER), U. S. Department of Energy, Grant No. DE-FG02-08ER64515. Supported in part through NASA / Oregon Space Grant Consortium, grant NNG05GJ85H.

  13. Peanut and tree nut allergy.

    PubMed

    Cox, Amanda; Sicherer, Scott H

    2015-01-01

    Allergy to peanut and tree nuts is a major worldwide health concern. The prevalence of these allergies may be increasing, but the reasons for these increases remain unclear. This group of foods accounts for a large proportion of severe and fatal food-allergic reactions. These allergies present most often during childhood but can occur at any age. Resolution is possible but uncommon, and frequent lifetime reactions caused by accidental ingestion are a serious problem. The major allergens of peanut and most tree nuts have been identified, allowing for insights into patient diagnoses, clinical outcomes, and potential future immunotherapies. PMID:26022873

  14. Tree Height Calculator: An Android App for Estimating Tree Height

    NASA Astrophysics Data System (ADS)

    Burca, V. S.; Htet, N. M.; Huang, X.; de Lanerolle, T. R.; Morelli, R.; Gourley, J. R.

    2011-12-01

    Conventionally, measuring tree height requires a collection of different tools - clinometer, transit, pencil, paper, laptop computer. Results are recorded manually and entered into a spreadsheet or database for future calculation and analysis. Tree Height Calculator is a mobile Android app the integrates the various steps in this process thereby improving the accuracy and dramatically reducing the time required to go from taking measurements to analyzing data. Given the user's height and the distance from the base of the tree (which can be downloaded into the app from a server), the app uses the phone's orientation sensor to calculate the angle of elevation. A simple trigonometric formula is then used to calculate and record the tree's height in the phone's database. When the phone has a WiFi connection, the data are transmitted to a server, from where they can be downloaded directly into a spreadsheet. The application was first tested in an Environmental Science laboratory at Trinity College. On the first trial, 103 data samples were collected, stored, and uploaded to the online database with only couple of dropped data points. On the second trial, 98 data samples were gathered with no loss of data. The app combined the individual measurements taken by the students in the lab, reducing the time required to produce a graph of the class's results from days to hours.

  15. SSSSave theave theave theave the TreesTreesTreesTrees!!!! Tree:Tree:Tree:Tree: Hackberry, Celtis Occidentalis L.Hackberry, Celtis Occidentalis L.Hackberry, Celtis Occidentalis L.Hackberry, Celtis Occidentalis L.

    E-print Network

    Ashline, George

    that bloom in May has leaves that turn from green to yellow in the fallhas leaves that turn from green to yellow in the fallhas leaves that turn from green to yellow in the fallhas leaves that turn from greenThe Lorax to youto youto youto you Then together we are going to think about what we use trees for and what

  16. Water Transport in Trees--An Artificial Laboratory Tree

    ERIC Educational Resources Information Center

    Susman, K.; Razpet, N.; Cepic, M.

    2011-01-01

    Water transport in tall trees is an everyday phenomenon, seldom noticed and not completely understood even by scientists. As a topic of current research in plant physiology it has several advantages for presentation within school physics lectures: it is interdisciplinary and clearly shows the connection between physics and biology; the…

  17. AncesTrees: ancestry estimation with randomized decision trees.

    PubMed

    Navega, David; Coelho, Catarina; Vicente, Ricardo; Ferreira, Maria Teresa; Wasterlain, Sofia; Cunha, Eugénia

    2015-09-01

    In forensic anthropology, ancestry estimation is essential in establishing the individual biological profile. The aim of this study is to present a new program--AncesTrees--developed for assessing ancestry based on metric analysis. AncesTrees relies on a machine learning ensemble algorithm, random forest, to classify the human skull. In the ensemble learning paradigm, several models are generated and co-jointly used to arrive at the final decision. The random forest algorithm creates ensembles of decision trees classifiers, a non-linear and non-parametric classification technique. The database used in AncesTrees is composed by 23 craniometric variables from 1,734 individuals, representative of six major ancestral groups and selected from the Howells' craniometric series. The program was tested in 128 adult crania from the following collections: the African slaves' skeletal collection of Valle da Gafaria; the Medical School Skull Collection and the Identified Skeletal Collection of 21st Century, both curated at the University of Coimbra. The first step of the test analysis was to perform ancestry estimation including all the ancestral groups of the database. The second stage of our test analysis was to conduct ancestry estimation including only the European and the African ancestral groups. In the first test analysis, 75% of the individuals of African ancestry and 79.2% of the individuals of European ancestry were correctly identified. The model involving only African and European ancestral groups had a better performance: 93.8% of all individuals were correctly classified. The obtained results show that AncesTrees can be a valuable tool in forensic anthropology. PMID:25053239

  18. Bayesian Detection in Bounded Height Tree Networks

    E-print Network

    Tay, Wee Peng

    We study the detection performance of large scale sensor networks, configured as trees with bounded height, in which information is progressively compressed as it moves towards the root of the tree. We show that, under a ...

  19. Bayesian Evidence Framework for Decision Tree Learning

    NASA Astrophysics Data System (ADS)

    Chatpatanasiri, Ratthachat; Kijsirikul, Boonserm

    2005-11-01

    This work is primary interested in the problem of, given the observed data, selecting a single decision (or classification) tree. Although a single decision tree has a high risk to be overfitted, the induced tree is easily interpreted. Researchers have invented various methods such as tree pruning or tree averaging for preventing the induced tree from overfitting (and from underfitting) the data. In this paper, instead of using those conventional approaches, we apply the Bayesian evidence framework of Gull, Skilling and Mackay to a process of selecting a decision tree. We derive a formal function to measure `the fitness' for each decision tree given a set of observed data. Our method, in fact, is analogous to a well-known Bayesian model selection method for interpolating noisy continuous-value data. As in regression problems, given reasonable assumptions, this derived score function automatically quantifies the principle of Ockham's razor, and hence reasonably deals with the issue of underfitting-overfitting tradeoff.

  20. Dealing with Storm-Damaged Trees 

    E-print Network

    Kirk, Melanie; Taylor, Eric; Foster, C. Darwin

    2005-10-25

    -Damaged Trees Melanie R. Kirk, Extension Program Specialist, Eric L. Taylor, Assistant Professor and Extension Specialist, and C. Darwin Foster, Associate Department Head and Extension Program Leader for Forestry, The Texas A&M University System Downed trees...

  1. The Shapley value of phylogenetic trees.

    PubMed

    Haake, Claus-Jochen; Kashiwada, Akemi; Su, Francis Edward

    2008-04-01

    Every weighted tree corresponds naturally to a cooperative game that we call a tree game; it assigns to each subset of leaves the sum of the weights of the minimal subtree spanned by those leaves. In the context of phylogenetic trees, the leaves are species and this assignment captures the diversity present in the coalition of species considered. We consider the Shapley value of tree games and suggest a biological interpretation. We determine the linear transformation M that shows the dependence of the Shapley value on the edge weights of the tree, and we also compute a null space basis of M. Both depend on the split counts of the tree. Finally, we characterize the Shapley value on tree games by four axioms, a counterpart to Shapley's original theorem on the larger class of cooperative games. We also include a brief discussion of the core of tree games. PMID:17805545

  2. Can These Trees Be Saved? (Spanish) 

    E-print Network

    Taylor, Eric; Foster, C. Darwin

    2007-10-08

    This publication explains how to determine whether a storm-damaged tree can be saved. If the damage is not severe, most trees will recover in time. Helpful illustrations depict different levels of damage....

  3. SEVEN TREES IN ONE Andreas Blass

    E-print Network

    Blass, Andreas R.

    elementary constructions can be extracted from ex­ istence proofs carried out in the much more liberal a depth to the empty tree, we assign 0.) As this terminology suggests, we visualize trees as growing

  4. Electric Trees and Pond Creatures.

    ERIC Educational Resources Information Center

    Weaver, Helen; Hounshell, Paul B.

    1978-01-01

    Two learning activities are presented to develop observation and classification skills at the elementary level. The first is an electric box that associates tree names with leaf and bark specimens, and the second is a pond water observation and slide preparation activity. (BB)

  5. Support Vector Machine Classification Trees.

    PubMed

    de Boves Harrington, Peter

    2015-11-01

    Proteomic and metabolomic studies based on chemical profiling require powerful classifiers to model accurately complex collections of data. Support vector machines (SVMs) are advantageous in that they provide a maximum margin of separation for the classification hyperplane. A new method for constructing classification trees, for which the branches comprise SVMs, has been devised. The novel feature is that the distribution of the data objects is used to determine the SVM encoding. The variance and covariance of the data objects are used for determining the bipolar encoding required for the SVM. The SVM that yields the lowest entropy of classification becomes the branch of the tree. The SVM-tree classifier has the added advantage that nonlinearly separable data may be accurately classified without optimization of the cost parameter C or searching for a correct higher dimensional kernel transform. It compares favorably to a regularized linear discriminant analysis, SVMs in a one against all multiple classifier, and a fuzzy rule-building expert system, a tree classifier with a fuzzy margin of separation. SVMs offer a speed advantage, especially for data sets that have more measurements than objects. PMID:26461495

  6. Logistic Model Trees Niels Landwehr

    E-print Network

    Frank, Eibe

    Logistic Model Trees Niels Landwehr Institute for Computer Science, University of Freiburg for classification problems, using logistic regression instead of linear regression. We use a stagewise fitting process to construct the logistic regression models that can select relevant attributes in the data

  7. Logistic Model Trees + Niels Landwehr

    E-print Network

    Frank, Eibe

    Logistic Model Trees + Niels Landwehr Institute for Computer Science, University of Freiburg for classification problems, using logistic regression instead of linear regression. We use a stagewise fitting process to construct the logistic regression models that can select relevant attributes in the data

  8. The Tree of Life Project

    ERIC Educational Resources Information Center

    Milbrath, Sherry

    2009-01-01

    Middle-school students are just beginning to recognize their place in the world. That is why this author believes it is important to incorporate their world into their art. In this article, the author discusses the "Tree of Life" project, which she developed for her students in order to make them aware of various environmental issues, and how to…

  9. Human Arterial-Tree Multiscale

    E-print Network

    Human Arterial- Tree Multiscale Simulations CRUNCH Group Division of Applied Mathematics Brown University George Karniadakis and Leopold Grinberg #12;For modeling purposes, we subdivide the arterial system into three levels: 1. The Macrovascular Network (MaN), which includes large arteries, down

  10. Not Just a Fall Tree

    ERIC Educational Resources Information Center

    Miller-Hewes, Kathy A.

    2004-01-01

    Trees burst with color in the northern states. Autumn leaves dust the ground. Painting the fall landscape is nothing new. Teachers have been doing it in classrooms for decades. The approach, however, can make the difference between whether the fall landscape is simply painting for fun, or a real learning experience. Students learn best when they…

  11. GumTree: Data reduction

    NASA Astrophysics Data System (ADS)

    Rayner, Hugh; Hathaway, Paul; Hauser, Nick; Fei, Yang; Franceschini, Ferdi; Lam, Tony

    2006-11-01

    Access to software tools for interactive data reduction, visualisation and analysis during a neutron scattering experiment enables instrument users to make informed decisions regarding the direction and success of their experiment. ANSTO aims to enhance the experiment experience of its facility's users by integrating these data reduction tools with the instrument control interface for immediate feedback. GumTree is a software framework and application designed to support an Integrated Scientific Experimental Environment, for concurrent access to instrument control, data acquisition, visualisation and analysis software. The Data Reduction and Analysis (DRA) module is a component of the GumTree framework that allows users to perform data reduction, correction and basic analysis within GumTree while an experiment is running. It is highly integrated with GumTree, able to pull experiment data and metadata directly from the instrument control and data acquisition components. The DRA itself uses components common to all instruments at the facility, providing a consistent interface. It features familiar ISAW-based 1D and 2D plotting, an OpenGL-based 3D plotter and peak fitting performed by fityk. This paper covers the benefits of integration, the flexibility of the DRA module, ease of use for the interface and audit trail generation.

  12. MYCOTOXINS IN EDIBLE TREE NUTS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Tree nuts (almonds, pistachios, and walnuts) are an exceptionally valuable crop, especially in California, with an aggregate value approaching $3.5 billion. Much of this economic value comes from overseas markets, with up to 60% of the crop being exported. The product can be contaminated with aflat...

  13. Millersville University's Trees of Distinction

    E-print Network

    Hardy, Christopher R.

    sycamore (Platanus acerifolia) 2014-05-22 #12;#16: Black walnut (Juglans nigra) 2014-05-22 #12;#17: Persian walnut (Juglans regia) 2014-05-22 #12;#18: Female Ginkgo, Maidenhair tree (Ginkgo biloba) 2014-06-14 #12

  14. Compartmentalization of Decay in Trees.

    ERIC Educational Resources Information Center

    Shigo, Alex L.

    1985-01-01

    Unlike animals, which heal, trees compartmentalize by setting boundaries that resist the spread of invading microorganisms. Discusses the creation of new walls by anatomical and chemical means in response to death of a branch or pruning. Points out that genetic control of compartmentalization has resulted from evolution of resistant species. (DH)

  15. The Education of Little Tree.

    ERIC Educational Resources Information Center

    Carter, Forrest

    First published in 1976, this autobiography contains Forrest Carter's--Little Tree's--remembrances of life with his Eastern Cherokee Hill country grandparents in the 1930s. There are 21 chapters, recounting humorous and serious episodes from a 5-year period and dealing with the themes of growing up, Indian life and values, family relationships,…

  16. Feathered Hollow in Living Tree

    USGS Multimedia Gallery

    Hollow in living tree in gallery forest of tributary to Tebicuary river. Grey feathers stuck along bottom of entrance suggest that it may be used by a bird. Paraguay is home to at least 589 breeding bird species and 120 migratory bird species. The Ñeembucú Region is typified by extensi...

  17. Cedar Tree in Bryce Canyon

    USGS Multimedia Gallery

    A cedar tree in Bryce Canyon National Park. Bryce Canyon is a unique sandstone formation in southern Utah. It is home to a large number of hoodoos, which are oddly shaped pillars of rock that formed due to different erosion rates for the dolomite that caps them and the sandstone that forms their ba...

  18. Can Children Read Evolutionary Trees?

    ERIC Educational Resources Information Center

    Ainsworth, Shaaron; Saffer, Jessica

    2013-01-01

    Representations of the "tree of life" such as cladograms show the history of lineages and their relationships. They are increasingly found in formal and informal learning settings. Unfortunately, there is evidence that these representations can be challenging to interpret correctly. This study explored the question of whether children aged 7-11…

  19. Trees of Our National Forests.

    ERIC Educational Resources Information Center

    Forest Service (USDA), Washington, DC.

    Presented is a description of the creation of the National Forests system, how trees grow, managing the National Forests, types of management systems, and managing for multiple use, including wildlife, water, recreation and other uses. Included are: (1) photographs; (2) line drawings of typical leaves, cones, flowers, and seeds; and (3)…

  20. The Trees that surround us

    NASA Astrophysics Data System (ADS)

    Costa, M. E. G.; Rodrigues, M. A. S.

    2012-04-01

    In our school the activities linked with sciences are developed in a partnership with other school subjects. Interdisciplinary projects are always valued from beginning to end of a project. It is common for teachers of different areas to work together in a Science project. Research of English written articles is very important not only for the development of our students' scientific literacy but also as a way of widening knowledge and a view on different perspectives of life instead of being limited to research of any articles in Portuguese language. In this study we are going to collect data about the predominant tree species in the region, especially the invasive trees from the acacia species, the native tree species and the commercial species. We are going to study the reasons for the appearance of each species and draw a chart of soil occupation in the council. This chart will also allow the study of the distribution and use of land for each tree species. This research work is the first stage for a contribution to warn the town council of the dangers of the invasive species to the future economy of the council.

  1. The Gift of the Tree

    ERIC Educational Resources Information Center

    Jones, Marla Wagner

    2009-01-01

    A piece of children's literature can be a powerful tool for teaching and learning science; however, it takes more than reading about a topic to qualify as "doing science." Inspired by the book, "The Gift of the Tree", the author developed an in-depth interdisciplinary lesson for her sixth-grade students without diluting the science. Through this…

  2. The Tree of Animal Life

    ERIC Educational Resources Information Center

    Braude, Stan

    2007-01-01

    In this article, the author describes a short activity which introduces third- to fifth-grade students to animal classification. The Tree of Animal Life activity is a simple, sorting exercise that can help them see a bigger picture. The activity sets the stage for learning about animal taxonomy and introduces the characteristics of various animal…

  3. Key for Trees of Iowa.

    ERIC Educational Resources Information Center

    Coder, Kim D.; Wray, Paul H.

    This key is designed to help identify the most common trees found in Iowa. It is based on vegetative characteristics such as leaves, fruits, and bark and is illustrated with black and white line drawings. Since vegetative characteristics vary due to climate, age, soil fertility, and other conditions, the numerical sizes listed, such as length and…

  4. Organic Fruit Tree Rick Foster

    E-print Network

    Pittendrigh, Barry

    of fruit · Sprays of sulfur provided some control · Sooty blotch and fly speck can be washed off #12;Apple fruit formation, regular sprays of sulfur every 10-14 days · Expect some culls #12;Insects and MitesOrganic Fruit Tree Production Rick Foster Department of Entomology Purdue University #12;Challenges

  5. Chopping Down the Cherry Tree.

    ERIC Educational Resources Information Center

    Griswold, Jerry

    1995-01-01

    Attempts once again to put to rest the infamous "I cannot tell a lie" episode involving George Washington and a downed cherry tree. Appends an editor's note that states that William Bennett's "The Children's Book of Virtues" which perpetuates this infamous piece of "fakelore." (RS)

  6. Decision-tree construction and analysis

    SciTech Connect

    Murphy, P.; Olson, B.H.

    1996-02-01

    Decision-tree construction and analysis indicate the quantitative occurrence of sulfate-reducing bacteria in a groundwater basin. The decision-tree analysis allowed determination of the most important measured variables (discriminators) associated with the occurrence of sulfate-reducing bacteria. Those variables may be used to improve reliability and validity of the tree. Statistical validation of endpoint data (leaves) is described, and decision trees are presented that increase the understanding of individual wells and hypothesize causes for sulfate-reducing bacteria.

  7. Collective Tree Spanners in Graphs with Bounded Genus, Chordality, Tree-Width,

    E-print Network

    Dragan, Feodor F.

    Collective Tree Spanners in Graphs with Bounded Genus, Chordality, Tree-Width, or Clique {dragan, cyan}@cs.kent.edu Abstract. In this paper we study collective additive tree spanners for special) admits a system of µ collective additive tree r-spanners if there is a system T (G) of at most µ spanning

  8. Inferring Species Trees Directly from Biallelic Genetic Markers: Bypassing Gene Trees in a Full Coalescent Analysis

    E-print Network

    Rosenberg, Noah

    Inferring Species Trees Directly from Biallelic Genetic Markers: Bypassing Gene Trees in a Full framework for estimating species trees and species demograph- ics from genetic markers. However, practical trees possible for each genetic marker. Here we describe a polynomial-time algorithm that computes

  9. Monitoring amenity tree health in England The Condition Survey of Non-woodland Amenity Trees

    E-print Network

    36 Monitoring amenity tree health in England The Condition Survey of Non-woodland Amenity Trees)-led survey which enables us to monitor the condition of amenity trees in parks, city streets and stately countries since 1987 (Hendry, 2005). Whilst the Forest Condition Survey concentrates on forest trees

  10. RELATING TREE PHYSIOLOGY TO PAST AND FUTURE CHANGES IN TROPICAL RAINFOREST TREE COMMUNITIES

    E-print Network

    Coley, Phyllis

    RELATING TREE PHYSIOLOGY TO PAST AND FUTURE CHANGES IN TROPICAL RAINFOREST TREE COMMUNITIES THOMAS. Predicting future changes in tropical rainforest tree communities requires a good under- standing of past to which tropical rainforest tree communities have changed during the last few thousand years has been

  11. DECISION TREE INDUCTION FOR DYNAMIC, HIGH-DIMENSIONAL DATA USING P-TREES1

    E-print Network

    Denton, Anne M.

    DECISION TREE INDUCTION FOR DYNAMIC, HIGH-DIMENSIONAL DATA USING P-TREES1 Anne Denton and William.Denton, William.Perrizo}@ndsu.nodak.edu Abstract1 Decision Tree Induction is a powerful classification tool be relevant. Decision tree branches are evaluated as needed, based on the most recent data, focusing entirely

  12. Totally Tree-mendous Activities: Projects To Discover the Beauty and Benefits of Trees.

    ERIC Educational Resources Information Center

    Hollister, Sarah

    This teacher's guide supplies information and hands-on activities to teach about trees from several disciplines. Activities are grouped into six areas that cover botany, social studies, arts and literature (aesthetics), and trees as a resource. Sections include: (1) Tree Identification, which defines trees and leaves and presents activities that…

  13. Discordance of Species Trees with Their Most Likely Gene Trees: A Unifying Principle

    E-print Network

    Rosenberg, Noah

    Letter Discordance of Species Trees with Their Most Likely Gene Trees: A Unifying Principle Noah A@stanford.edu. Associate editor: Barbara Holland Abstract A labeled gene tree topology that disagrees with a labeled species tree topology is said to be anomalous if it is more probable under a coalescent model for gene

  14. A Characterization of the Set of Species Trees that Produce Anomalous Ranked Gene Trees

    E-print Network

    Rosenberg, Noah

    A Characterization of the Set of Species Trees that Produce Anomalous Ranked Gene Trees James H. Degnan, Noah A. Rosenberg, and Tanja Stadler Abstract--Ranked gene trees, which consider both the gene tree topology and the sequence in which gene lineages separate, can potentially provide a new source

  15. RESOVING THE GENE TREE AND SPECIES TREE PROBLEM BY PHYLOGENETIC MINING

    E-print Network

    Wong, Limsoon

    RESOVING THE GENE TREE AND SPECIES TREE PROBLEM BY PHYLOGENETIC MINING XIAOXU HAN Department of Mathematics and Bioinformatics Program, Eastern Michigan University Ypsilanti, MI 48197, USA The gene tree and species tree problem remains a central problem in phylogenomics. To overcome this problem, gene

  16. The Influence of Iron and Zinc Supplementation on the Bioavailability of Provitamin A Carotenoids from Papaya Following Consumption of a Vitamin A-Deficient Diet.

    PubMed

    Kana-Sop, Marie Modestine; Gouado, Inocent; Achu, Mercy Bih; Van Camp, John; Amvam Zollo, Paul Henri; Schweigert, Florian J; Oberleas, Donald; Ekoe, Tetanye

    2015-01-01

    Iron deficiency anemia, zinc and vitamin A deficiencies are serious public health problems in Cameroon, as in many developing countries. Local vegetables which are sources of provitamin A carotenoids (PACs) can be used to improve vitamin A intakes. However, traditional meals are often unable to cover zinc and iron needs. The aim of this study was to determine the bioavailability of 3 PACs (?-carotene, ?-carotene, and ?-cryptoxanthin) in young men, who were fed with a vitamin A-free diet and received iron and zinc supplementation. Twelve healthy participants were divided into three groups and were supplemented with elemental iron (20 mg of iron fumarate), 20 mg of zinc sulfate or iron+zinc (20 mg of iron in the morning and 20 mg of zinc in the evening) for 11 d. They were given a vitamin A- and PAC-free diet from the 6th to the 11th day, followed by a test meal containing 0.55 kg of freshly peeled papaya as a source of PACs. Blood samples were collected four times successively on the 11th day (the test meal day), at T0 (just after the test meal), after 2 h (T2), after 4 h (T4) and after 7 h (T7). Ultracentrifugation was used to isolate serum chylomicrons. Retinol appearance and PAC postprandial concentrations were determined. The supplementation with zinc, iron and iron+zinc influenced the chylomicron appearance of retinol and PACs differently as reflected by retention times and maximum absorption peaks. Iron led to highest retinol levels in the chylomicron. Zinc and iron+zinc supplements were best for optimal intact appearance of ?-carotene, ?-carotene and ?-cryptoxanthin respectively. Supplementation with iron led to the greatest bioavailability of PACs from papaya and its conversion to retinol. PMID:26226956

  17. Kinetic Medians and -Trees Pankaj K. Agarwal

    E-print Network

    Agarwal, Pankaj K.

    Kinetic Medians and -Trees Pankaj K. Agarwal½ , Jie Gao¾ , and Leonidas J. Guibas¾ ½ Department move, we use event-based kinetic data structures to update the tree when necessary. Both trees undergo on the query time [26,25, 19] or are too complicated [1, 15]. In this paper we develop kinetic data structures

  18. Compound WordsCompound WordsCompound WordsCompound Words Crabapple TreeCrabapple TreeCrabapple TreeCrabapple Tree

    E-print Network

    Ashline, George

    J.C. 2004 Compound WordsCompound WordsCompound WordsCompound Words Crabapple TreeCrabapple Tree the mixture of two or more different things. Therefore, a compound word is the combination of two different words to form a new word. An example of such a word is "crabapple." Go to the crabapple tree. Observe

  19. Street Tree Inventory Report and Recommendations

    E-print Network

    and planting. * Conduct an annual Arbor Day celebration and involve local groups. * Craft a public tree care ...................................................................6 Street Tree Care Condition ...........................................................7 than 24" diameter. * Two-thirds of street trees are in good condition and 59% require only routine care

  20. University of Oregon Atlas of Trees

    E-print Network

    Cina, Jeff

    University of Oregon Atlas of Trees Maps created by the UO InfoGraphics Lab, Department of Geography #12;University of Oregon Atlas of Trees Web Version ­ July 2012 The printed version of the Atlas of Trees was first produced in 1996

  1. Random trees Jean-Franois Le Gall

    E-print Network

    Le Gall, Jean-François

    Random trees Jean-François Le Gall Université Paris-Sud Orsay and Institut universitaire de France IMS Annual Meeting, Göteborg, August 2010 Jean-François Le Gall (Université Paris-Sud) Random trees Göteborg 1 / 40 #12;Outline Trees are mathematical objects that play an important role in several areas

  2. 7 CFR 1214.3 - Christmas tree.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 10 2013-01-01 2013-01-01 false Christmas tree. 1214.3 Section 1214.3 Agriculture... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE CHRISTMAS TREE PROMOTION, RESEARCH, AND INFORMATION ORDER Christmas Tree Promotion, Research, and Information Order Definitions §...

  3. Remarks about Hardy inequalities on metric trees

    E-print Network

    Tomas Ekholm; Rupert L. Frank; Hynek Kovarik

    2007-11-13

    We find sharp conditions on the growth of a rooted regular metric tree such that the Neumann Laplacian on the tree satisfies a Hardy inequality. In particular, we consider homogeneous metric trees. Moreover, we show that a non-trivial Aharonov-Bohm magnetic field leads to a Hardy inequality on a loop graph.

  4. "These trees were presented to ONRC by

    E-print Network

    Brown, Sally

    "These trees were presented to ONRC by Fred Rosmond who propagated them from acorns he collected. Oregon White Oak is the only native oak of Washington. As these trees grow we will be reminded of Fred." These words are etched upon a modest bronze plate which stands before the two thriving Oregon White Oak trees

  5. Forest Research Much more than trees

    E-print Network

    Forest Research Much more than trees #12;Welcome to Forest Research. We are the research agency much more than trees ­ it's about adapting to, and mitigating the risks from, climate change and land-use management, whose work focuses on the use of applied science for tree, woodland and forest

  6. 7 CFR 1214.3 - Christmas tree.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 10 2012-01-01 2012-01-01 false Christmas tree. 1214.3 Section 1214.3 Agriculture... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE CHRISTMAS TREE PROMOTION, RESEARCH, AND INFORMATION ORDER Christmas Tree Promotion, Research, and Information Order Definitions §...

  7. 7 CFR 1214.3 - Christmas tree.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 10 2014-01-01 2014-01-01 false Christmas tree. 1214.3 Section 1214.3 Agriculture... AND ORDERS; MISCELLANEOUS COMMODITIES), DEPARTMENT OF AGRICULTURE CHRISTMAS TREE PROMOTION, RESEARCH, AND INFORMATION ORDER Christmas Tree Promotion, Research, and Information Order Definitions §...

  8. Simplifying gene trees for easier comprehension

    PubMed Central

    Lott, Paul-Ludwig; Mundry, Marvin; Sassenberg, Christoph; Lorkowski, Stefan; Fuellen, Georg

    2006-01-01

    Background In the genomic age, gene trees may contain large amounts of data making them hard to read and understand. Therefore, an automated simplification is important. Results We present a simplification tool for gene trees called TreeSimplifier. Based on species tree information and HUGO gene names, it summarizes "monophyla". These monophyla correspond to subtrees of the gene tree where the evolution of a gene follows species phylogeny, and they are simplified to single leaves in the gene tree. Such a simplification may fail, for example, due to genes in the gene tree that are misplaced. In this way, misplaced genes can be identified. Optionally, our tool glosses over a limited degree of "paraphyly" in a further simplification step. In both simplification steps, species can be summarized into groups and treated as equivalent. In the present study we used our tool to derive a simplified tree of 397 leaves from a tree of 1138 leaves. Comparing the simplified tree to a "cartoon tree" created manually, we note that both agree to a high degree. Conclusion Our automatic simplification tool for gene trees is fast, accurate, and effective. It yields results of similar quality as manual simplification. It should be valuable in phylogenetic studies of large protein families. The software is available at . PMID:16643669

  9. MITCHEL RESNICK THINKING LIKE A TREE

    E-print Network

    Resnick, Mitchel

    ." It is a strange looking tree. At the base of the tree is a tangle of roots, rising about a meter above the ground in the biological world, used not only by walking trees but many other plants and animals as well. Ecological

  10. Linear Machine Decision Trees Paul E. Utgoff

    E-print Network

    Utgoff, Paul

    Linear Machine Decision Trees Paul E. Utgoff Carla E. Brodley Department of Computer Science Abstract This article presents an algorithm for inducing multiclass decision trees with multivariate tests a classifier in the form of a decision tree, using a combination of methods from connectionist and decision

  11. Decision Trees for Functional Variables Suhrid Balakrishnan

    E-print Network

    Decision Trees for Functional Variables Suhrid Balakrishnan Department of Computer Science Rutgers an intuitive scheme for ex- tending decision trees to handle functional input variables. Our results show that such decision trees are both accurate and readily interpretable. 1 INTRODUCTION We present an extension

  12. k+ DECISION TREES JAMES ASPNES 1

    E-print Network

    O'Donnell, Ryan

    k+ DECISION TREES JAMES ASPNES 1 AND ERIC BLAIS 2 AND MURAT DEMIRBAS 3 AND RYAN O'DONNELL 2 of computation for boolean functions: the 2+ decision tree. This model is an augmentation of the standard decision tree model: now each internal node queries an arbitrary set of literals and branches on whether 0

  13. Avoiding Differences Spanning Trees in Grid Graphs

    E-print Network

    Zeilberger, Doron

    Avoiding Differences Spanning Trees in Grid Graphs The Firefighter Problem Automated Proof and Discovery #12;Avoiding Differences Spanning Trees in Grid Graphs The Firefighter Problem Automated Proof and Discovery #12;Avoiding Differences Spanning Trees in Grid Graphs The Firefighter Problem Outline 1 Avoiding

  14. Street Tree Inventory Report and Recommendations

    E-print Network

    that encroach into clear zones above streets and sidewalks. * Street trees in Missouri City are valued at more than $44 million. Recommendations include: * Favor shade trees other than live oak when designing by the Texas Forest Service. It is adapted from the Street Tree Management Tool for Urban Forest Managers

  15. 7 CFR 783.3 - Definitions.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ...annual crop is produced for commercial purposes, such as maple tree for syrup, papaya tree, or orchard tree. Plantain and banana plants are also included. Trees used for pulp or timber are not considered eligible trees under this part....

  16. Big Tree of the Month -September 2013 Anne Krantz, Tree Steward and NH Big Tree Committee

    E-print Network

    New Hampshire, University of

    Sassafras (Sassafras albidum) ­ Part II We all love the colorful leaves of fall in New England. Among the brilliant leaves are the yellow to red leaves of native sassafras. Its unique mitten shaped leaves are fun. These strange leaves with unusual smooth edges make identifying sassafras easy, but it is not a common tree

  17. Tree-grass and tree-tree interactions in a temperate savanna 

    E-print Network

    Simmons, Mark Trevor

    2004-11-15

    . The maintenance and formation of savannas have been attributed to climate, soils, herbivory and fire. However, the reasons for the coexistence of trees and the grass layer have still to be determined. These two contrasting life forms create a complex of intra...

  18. RECONCILED TREES 1 Submitted to Molecular Phylogeny and Evolution

    E-print Network

    Page, Roderic

    RECONCILED TREES 1 Submitted to Molecular Phylogeny and Evolution Please address all correspondence trees From Gene to Organismal Phylogeny: Reconciled Trees and the Gene Tree/Species Tree Problem RODERIC of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, U.K. #12;RECONCILED TREES 2

  19. Trees 1 2004 Goodrich, Tamassia Make Money Fast!

    E-print Network

    Alechina, Natasha

    Trees 1© 2004 Goodrich, Tamassia Trees Make Money Fast! Stock Fraud Ponzi Scheme Bank Robbery #12;Trees 2© 2004 Goodrich, Tamassia What is a Tree In computer science, a tree is an abstract model of a hierarchical structure A tree consists of nodes with a parent-child relation (at most one parent!) Applications

  20. The Role of Trees in Seattle's Stormwater Management

    E-print Network

    Brown, Sally

    Urban Forest Symposium May 2015 #12;Overview 1. Stormwater 2. Stormwater + Trees 3. Stormwater + Trees #12;Forest Standard #12;Urban Areas #12;Stormwater + Trees #12;Effects of Trees on Stormwater 3 depends on: · Canopy cover · Climate · Deciduous / evergreen #12;Canopy Cover Few Trees Abundant Trees