Sample records for par fsh recombinante

  1. Facteurs prédictifs de succès de l’induction d’ovulation par FSH recombinante : résultats de l’étude Indigo

    Microsoft Academic Search

    I. Cédrin-Durnerin; J. Belaisch-Allart; C. Avril; J.-L. Pouly

    2010-01-01

    IntroductionAlthough ovulation induction is commonly used to treat infertility, few data are available concerning this treatment. Therefore, the aim of this prospective observational study was to describe medical practices and to identify predictive outcome factors of ovarian stimulation by recombinant FSH (r-hFSH), administrated with a self-injector pen.

  2. Follicle-stimulating hormone (FSH) blood test

    MedlinePLUS

    ... of tumor in the pituitary gland Due to Turner syndrome Low FSH levels in women may be ... by alcohol abuse, chemotherapy, or radiation Problems with genes, such as Klinefelter syndrome Treatment with hormones Certain ...

  3. Clinical Pharmacology of Recombinant Human Follicle-Stimulating Hormone (FSH). I. Comparative Pharmacokinetics with Urinary Human FSH

    Microsoft Academic Search

    Jean-Yves le Cotonnec; Hervé Claude Porchet; Vanya Beltrami; Azhar Khan; Steeve Toon; Malcolm Rowland

    1998-01-01

    Objective: To assess and compare the pharmacokinetics of recombinant human FSH with those of a reference preparation of urinary human FSH.Design: Urinary human FSH and recombinant human FSH (Metrodin and Gonal-F; Laboratoires Serono, Aubonne, Switzerland) were administered in a balanced, random order, crossover sequence as a single IV dose of 150 or 300 IU separated by 1 week of washout

  4. Primate FSH-receptor promoter nucleotide sequence heterogeneity affects FSH-receptor transcription

    Microsoft Academic Search

    Maik Brune; Christian Adams; Jörg Gromoll

    2010-01-01

    Follicle-stimulating hormone (FSH) is essential for primate reproduction and acts via the FSH-receptor (FSHR). Although the FSHR expression is highly cell-specific, knowledge of the FSHR promoter and its transcriptional regulation is very limited. We applied a comparative genomic approach of important primate lineages to characterize the FSHR core promoter region.The core promoter sequences of the human and different primate species

  5. Molecular cloning and functional analysis of the goose FSH? gene.

    PubMed

    Huang, Z; Li, X; Li, Y; Liu, R; Chen, Y; Wu, N; Wang, M; Song, Y; Yuan, X; Lan, L; Xu, Q; Chen, G; Zhao, W

    2015-06-01

    The objective of this investigation was to clone goose FSH?-subunit cDNA and to construct a FSH fusion gene to identify the function of FSH? mRNA during stages of the breeding cycle. The FSH? gene was obtained by reverse transcription-PCR, and the full-length FSH? mRNA sequence was amplified by rapid-amplification of cDNA ends. FSH? mRNA expression was detected in reproductive tissues at different stages (pre-laying, laying period, and broody period). Additionally, the expression of 4 genes known to be involved in reproduction (FSH?, GnRH, GH, and BMP) were evaluated in COS-7 cells expressing the fusion gene (pVITRO2-FSH??-CTP). The results show that the FSH? gene consists of a 16 base pair (bp) 5'-untranslated region (UTR), 396 bp open reading frame, and alternative 3'-UTRs at 518 bp and 780 bp, respectively. qPCR analyses revealed that FSH? mRNA is highly transcribed in reproductive tissues, including the pituitary, hypothalamus, ovaries, and oviduct. FSH? mRNA expression increased and subsequently decreased in the pituitary, ovaries, and oviduct during the reproductive stages. Stable FSH expression was confirmed using enzyme-linked immunosorbent assays after transfection with the pVITRO2-FSH??-CTP plasmid. FSH?, GnRH, and BMP expression increased significantly 36 h and 48 h after transfection with the fusion gene in COS-7 cells. The results demonstrate that the FSH? subunit functions in the goose reproductive cycle and provides a theoretical basis for future breeding work. PMID:25719958

  6. Ovarian function and FSH receptor characteristics during canine anoestrus.

    PubMed

    McBride, M W; Aughey, E; O'Shaughnessy, P J; Jeffcoate, I A

    2001-01-01

    Ovaries of bitches are relatively inactive during anoestrus despite apparently adequate circulating FSH concentrations. Alternative FSH receptor (FSH-R) transcripts in bitches might hinder the follicular response to gonadotrophins, which may account for anoestrus. The expression of the full length FSH-R and novel isoforms in bitches was examined using in situ hybridization and RT-PCR analysis. Various PCR primers to the FSH-R were used and its expression was assessed in ovarian tissue at different stages of the oestrous cycle. RT-PCR amplification of the extracellular domain (exon 1-10) was generally successful, indicating that cFSH-R expression (> 90%) occurs throughout the oestrous cycle. Two FSH-R isoforms were sequenced, but there were no clear differences in the pattern of expression between anoestrus and other stages of the oestrous cycle, except that isoform expression was less frequent (30% occurrence) in prepubertal bitches. Data from in situ hybridization showed clear expression of the FSH-R in secondary and antral follicles, and corpora lutea. It was concluded that there is no evidence of a change in the expression of the FSH-R specific to anoestrus. PMID:11787165

  7. Baculovirus et Thrapie CNRS UPS3044 Production de Protines Recombinantes

    E-print Network

    van Tiggelen, Bart

    Baculovirus et Thérapie CNRS UPS3044 Production de Protéines Recombinantes en cellules d'insectes Anticorps recombinants Martine Cérutti CNRS UPS3044, Baculovirus et Thérapie « Les insectes au service des hommes » Colloque Bioprocédés et Bioproduction 10 juillet 2014 PARIS Colloque Bioprocédés et

  8. Avian LH and FSH: comparison of several radioimmunoassays

    SciTech Connect

    Goldsmith, A.R.; Follett, B.K.

    1983-04-01

    Comparisons were made between various LH and FSH radioimmunoassays currently being used to measure avian hormones. The two LH assays were the homologous chicken system of Follett et al. (1972) and the turkey assay of Burke et al. (1979). These assays were also used in heterologous arrangement by interchanging the iodinated LH fractions and antisera. Five FSH assays were analyzed: two homologous chicken systems (Scanes et al., 1977; Sakai and Ishii, 1980) an assay based on mammalian materials (rat FSH and anti-ovine FSH antiserum, and one using labelled turkey FSH (Burke et al., 1979) with an anti-chicken FSH antiserum. The potencies of purified chicken and turkey gonadotrophin preparations and of a range of plasma samples from Japanese quail were measured in each assay. The two LH systems showed some degree of species specificity, such that chicken LH was more active than turkey LH in the chicken assay, whereas the reverse was true in the turkey LH assay. The potency estimates of the purified hormones in the various FSH assays were very consistent. The qualitative changes in plasma hormone levels were similar in all assays, although there were some differences in the magnitude of the responses.

  9. Impairing Follicle-Stimulating Hormone (FSH) Signaling in vivo: Targeted Disruption of the FSH Receptor Leads to Aberrant Gametogenesis and Hormonal Imbalance

    Microsoft Academic Search

    Andree Dierich; M. Ram Sairam; Lucia Monaco; Gian Maria Fimia; Anne Gansmuller; Marianne Lemeur; Paolo Sassone-Corsi

    1998-01-01

    Pituitary gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone stimulate the gonads by regulating germ cell proliferation and differentiation. FSH receptors (FSH-Rs) are localized to testicular Sertoli cells and ovarian granulosa cells and are coupled to activation of the adenylyl cyclase and other signaling pathways. Activation of FSH-Rs is considered essential for folliculogenesis in the female and spermatogenesis in the male.

  10. Superovulation of holstein heifers by a single subcutaneous injection of FSH dissolved in polyvinylpyrrolidone

    Microsoft Academic Search

    T. Takedomi; Y. Aoyagi; M. Konishi; H. Kishi; K. Taya; G. Watanabe; S. Sasamoto

    1995-01-01

    This study was undertaken to determine whether a single injection of porcine FSH (pFSH) would induce a superovulatory response in cattle. Holstein heifers were given a single injection of pFSH (30mg, sc) dissolved in saline (Group 1, n = 5); 50% polyvinylpyrrolidone (PVP; Group 2, n = 5); or 25% PVP (Group 3, n = 4). Group-4 heifers (n =

  11. Fsh Controls Gene Expression in Fish both Independently of and through Steroid Mediation

    E-print Network

    Paris-Sud XI, Université de

    Fsh Controls Gene Expression in Fish both Independently of and through Steroid Mediation Elisabeth to understand if Fsh effects are mediated through the production of steroids. To address this issue we performed4-steroids. What is more, most of the genes regulated by Fsh through steroid mediation were

  12. Allosteric activation of the follicle-stimulating hormone (FSH) receptor by selective, nonpeptide agonists.

    PubMed

    Yanofsky, Stephen D; Shen, Emily S; Holden, Frank; Whitehorn, Erik; Aguilar, Barbara; Tate, Emily; Holmes, Christopher P; Scheuerman, Randall; MacLean, Derek; Wu, May M; Frail, Donald E; López, Francisco J; Winneker, Richard; Arey, Brian J; Barrett, Ronald W

    2006-05-12

    The pituitary glycoprotein hormones, luteinizing hormone and follicle-stimulating hormone (FSH), act through their cognate receptors to initiate a series of coordinated physiological events that results in germ cell maturation. Given the importance of FSH in regulating folliculogenesis and fertility, the development of FSH mimetics has been sought to treat infertility. Currently, purified and recombinant human FSH are the only FSH receptor (FSH-R) agonists available for infertility treatment. By screening unbiased combinatorial chemistry libraries, using a cAMP-responsive luciferase reporter assay, we discovered thiazolidinone agonists (EC50's = 20 microm) of the human FSH-R. Subsequent analog library screening and parallel synthesis optimization resulted in the identification of a potent agonist (EC50 = 2 nm) with full efficacy compared with FSH that was FSH-R-selective and -dependent. The compound mediated progesterone production in Y1 cells transfected with the human FSH-R (EC50 = 980 nm) and estradiol production from primary rat ovarian granulosa cells (EC50 = 10.5 nm). This and related compounds did not compete with FSH for binding to the FSH-R. Use of human FSH/thyroid-stimulating hormone (TSH) receptor chimeras suggested a novel mechanism for receptor activation through a binding site independent of the natural hormone binding site. This study is the first report of a high affinity small molecule agonist that activates a glycoprotein hormone receptor through an allosteric mechanism. The small molecule FSH receptor agonists described here could lead to an oral alternative to the current parenteral FSH treatments used clinically to induce ovarian stimulation for both in vivo and in vitro fertilization therapy. PMID:16540466

  13. Effect of LH and FSH on vanadium distribution in hypophysectomized rats

    SciTech Connect

    Peabody, R.A.; Wallach, S.; Verch, R.L.; Lifschitz, M.

    1980-11-01

    The effect of LH and FSH on radiovanadium (/sup 48/V) metabolism in testis was explored by administering LH and/or FSH daily to hypophysectomized (HYPOX) rats prior to and after /sup 48/V injection. Whole body, serum, and tissue contents of /sup 48/V of HYPOX rats were not affected by LH or FSH except in testis. In testis, there were both independent and additive effects of LH and FSH on testicular weight and /sup 48/V content, expressed as either content per gram or per organ. LH was more active than FSH. The usual fourfold increase in /sup 48/V content per gram seen in HYPOX testis was reduced 50% by LH + FSH. These data demonstrate that gonadotropins affect the /sup 48/V content of the testes in HYPOX rats in the absence of growth hormone, adrenal steroids, and thyroxine.

  14. Fsh Controls Gene Expression in Fish both Independently of and through Steroid Mediation

    PubMed Central

    Sambroni, Elisabeth; Lareyre, Jean-Jacques; Le Gac, Florence

    2013-01-01

    The mechanisms and the mediators relaying Fsh action on testicular functions are poorly understood. Unlike in mammals, in fish both gonadotropins (Fsh and Lh) are able to efficiently stimulate steroidogenesis, likely through a direct interaction with their cognate receptors present on the Leydig cells. In this context, it is crucial to understand if Fsh effects are mediated through the production of steroids. To address this issue we performed transcriptome studies after in vitro incubations of rainbow trout testis explants in the presence of Fsh alone or in combination with trilostane, an inhibitor of ?4- steroidogenesis. Trilostane significantly reduced or suppressed the response of many genes to Fsh (like wisp1, testis gapdhs, cldn11, inha, vt1 or dmrt1) showing that, in fish, important aspects of Fsh action follow indirect pathways and require the production of ?4-steroids. What is more, most of the genes regulated by Fsh through steroid mediation were similarly regulated by Lh (and/or androgens). In contrast, the response to Fsh of other genes was not suppressed in the presence of trilostane. These latter included genes encoding for anti-mullerian hormone, midkine a (pleiotrophin related), angiopoietine-related protein, cyclins E1 and G1, hepatocyte growth factor activator, insulin-like growth factor 1b/3. A majority of those genes were preferentially regulated by Fsh, when compared to Lh, suggesting that specific regulatory effects of Fsh did not depend on steroid production. Finally, antagonistic effects between Fsh and steroids were found, in particular for genes encoding key factors of steroidogenesis (star, hsd3b1, cyp11b2-2) or for genes of the Igf system (igf1b/3). Our study provides the first clear evidence that, in fish, Fsh exerts ?4-steroid-independent regulatory functions on many genes which are highly relevant for the onset of spermatogenesis. PMID:24194844

  15. Fsh controls gene expression in fish both independently of and through steroid mediation.

    PubMed

    Sambroni, Elisabeth; Lareyre, Jean-Jacques; Le Gac, Florence

    2013-01-01

    The mechanisms and the mediators relaying Fsh action on testicular functions are poorly understood. Unlike in mammals, in fish both gonadotropins (Fsh and Lh) are able to efficiently stimulate steroidogenesis, likely through a direct interaction with their cognate receptors present on the Leydig cells. In this context, it is crucial to understand if Fsh effects are mediated through the production of steroids. To address this issue we performed transcriptome studies after in vitro incubations of rainbow trout testis explants in the presence of Fsh alone or in combination with trilostane, an inhibitor of ?4- steroidogenesis. Trilostane significantly reduced or suppressed the response of many genes to Fsh (like wisp1, testis gapdhs, cldn11, inha, vt1 or dmrt1) showing that, in fish, important aspects of Fsh action follow indirect pathways and require the production of ?4-steroids. What is more, most of the genes regulated by Fsh through steroid mediation were similarly regulated by Lh (and/or androgens). In contrast, the response to Fsh of other genes was not suppressed in the presence of trilostane. These latter included genes encoding for anti-mullerian hormone, midkine a (pleiotrophin related), angiopoietine-related protein, cyclins E1 and G1, hepatocyte growth factor activator, insulin-like growth factor 1b/3. A majority of those genes were preferentially regulated by Fsh, when compared to Lh, suggesting that specific regulatory effects of Fsh did not depend on steroid production. Finally, antagonistic effects between Fsh and steroids were found, in particular for genes encoding key factors of steroidogenesis (star, hsd3b1, cyp11b2-2) or for genes of the Igf system (igf1b/3). Our study provides the first clear evidence that, in fish, Fsh exerts ?4-steroid-independent regulatory functions on many genes which are highly relevant for the onset of spermatogenesis. PMID:24194844

  16. Characterization of tilapia FSH? gene and analysis of its 5? flanking region

    Microsoft Academic Search

    H. Rosenfeld; B. Levavi-Sivan; G. Gur; P. Melamed; I. Meiri; Z. Yaron; A. Elizur

    2001-01-01

    The objective of the current study was to unveil molecular mechanisms underlying transcriptional regulation of the FSH? gene expression in the pituitary of tilapia (Oreochromis mossambicus). The full-length sequence of tilapia FSH? (tFSH?) gene was determined. Its transcriptional unit (2.7 kb) exhibits the conserved genomic organization, i.e. three exons and two introns. Primer extension and RT-PCR analysis revealed heterogeneity of

  17. Percentage change of FSH value: new variable to predict the seminal outcome after varicocelectomy.

    PubMed

    Cantoro, U; Catanzariti, F; Lacetera, V; Quaresima, L; Giovanni, M; Polito, M

    2015-05-01

    In the literature, there is no good agreement with variables to predict seminal outcome after varicocelectomy. The purpose of this study was to evaluate the FSH percentage change (?% FSH) as a predictor of the seminal outcome after varicocelectomy together with other known predictors. We evaluated 118 patients who underwent varicocelectomy. We assessed factors that could be predictors of the improvement of semen characteristics: LH, FSH, total and free testosterone before operation, testis volume, age, testicular pain, body mass index (BMI), ?% FSH, varicocele Doppler ultrasound grade using regression analysis. Mean sperm concentration increased from 27 ± 12 to 52 ± 15 million per ml post-operatively (P < 0.003), mean sperm motility increased from 31 ± 12 to 40 ± 13 million per ml following the operation (P < 0.02), also mean FSH value changed from 10.2 ± 8.3 to 6.7 ± 7.2 UI/L (P < 0.01). In unvaried regression analysis, ?% FSH, BMI and age were predictors of the improvement of semen characteristics. In multiple regression analysis, only ?% FSH and age were predictors. Negative correlation with age and positive correlation with ?% FSH were observed. Our findings suggest that ?% FSH (before and after varicocelectomy) and age are significant factors predicting the improvement of semen characteristics. PMID:24698207

  18. Pharmacokinetics and follicular dynamics of corifollitropin alfa versus recombinant FSH during ovarian stimulation for IVF.

    PubMed

    Fauser, Bart C J M; Alper, Michael M; Ledger, William; Schoolcraft, William B; Zandvliet, Anthe; Mannaerts, Bernadette M J L

    2010-11-01

    A single injection of corifollitropin alfa can replace seven daily injections of recombinant FSH (rFSH) using a gonadotrophin-releasing hormone antagonist protocol in ovarian stimulation prior to IVF or intracytoplasmic sperm injection. This double-blind randomized controlled trial assessed the pharmacokinetics and pharmacodynamics of 150?g corifollitropin alfa versus daily 200IU rFSH in 1509 patients. Comparative analyses were performed on serum concentrations of FSH immunoreactivity (pharmacokinetics), and the number and size of growing follicles, and inhibin B and oestradiol concentrations as biomarkers of ovarian response (pharmacodynamics). The rate of follicular development was similar in both treatment groups. By stimulation day 8, 33% of patients treated with corifollitropin alfa reached the criterion for human chorionic gonadotrophin (HCG) injection. The number of follicles ?11mm was slightly higher after corifollitropin alfa compared with daily rFSH at stimulation day 8 (difference, 1.2; 95% confidence interval (CI) 0.5-1.8; P<0.01) and on the day of HCG injection (difference, 2.1; 95% CI 1.4-2.8; P<0.01). The rise of inhibin B and oestradiol concentrations was similar in both treatment groups. Although the pharmacokinetics of corifollitropin alfa and rFSH are quite different their pharmacodynamic profiles at the dosages used are similar. A single injection of corifollitropin alfa can replace seven daily injections of recombinant FSH (rFSH) using a gonadotrophin-releasing hormone antagonist protocol in ovarian stimulation prior to IVF or intracytoplasmic sperm injection. The objective of this study was to compare the pharmacokinetics and pharmacodynamics of corifollitropin alfa versus daily rFSH. A total of 1509 patients were randomized in a double-blind, controlled trial to either a single injection of 150?g corifollitropin alfa or to daily injections of 200IU rFSH for the first 7 days of ovarian stimulation. Serum levels of FSH immunoreactivity were analysed (pharmacokinetic analysis), together with the number and size of growing follicles and serum inhibin B and oestradiol concentrations as biomarkers of the ovarian response (pharmacodynamic analysis). Serum FSH immunoreactivity levels were higher up to stimulation day 5 for corifollitropin alfa compared with the daily rFSH regimen but were similar from day 8 onwards, when patients started rFSH if the criteria for human chorionic gonadotrophin were not yet reached. Corifollitropin alfa treatment resulted in a similar growth rate of follicles though a slightly higher number of follicles were recruited compared with daily rFSH. It is concluded that the pharmacokinetics of corifollitropin alfa and rFSH are quite different but their induced pharmacodynamic effects at the dosages used are similar. PMID:20843746

  19. Comparison of Specificity and Sensitivity of AMH and FSH in Diagnosis of Premature Ovarian Failure

    PubMed Central

    Alipour, Farzaneh; Rasekhjahromi, Athar; Maalhagh, Mehrnoosh; Sobhanian, Saeid; Hosseinpoor, Masoumeh

    2015-01-01

    Introduction. Anti-Müllerian hormone represents the primitive follicular number and ovarian age. Low level of AMH is in relation to early menopausal state and decreased ovarian reserve. AMH level changes occur prior to FSH level in representing ovarian failure. The aim of this study is to compare sensitivity and specificity of AMH with FSH in diagnosis of POF. Material and Methods. This descriptive study is done on 96 patients referred to Dr. Rasekh Clinic. Serum level of AMH and FSH was measured at Day 3 (3rd day of menstrual cycle) and data were analyzed through SPSS 21 software. Results. Results of AMH and FSH serum level indicate that AMH has more sensitivity (80% versus 28.57%) and almost equal specificity (78.89% versus 78.65%) compared with FSH. Also negative predictive value of AMH (98.61%) and FSH (87.5%) is different. But positive predictive value is the same (17.39%). Diagnostic accuracy of AMH is more than FSH and has significant differences. Conclusion. According to the results of this study, AMH serum level is more sensitive than FSH serum level. Also AMH has more negative predictive value. Besides, this hormone can be measured at any time of menstrual cycle, against FSH. AMH seems to be more useful in early diagnosis of POF. PMID:26113766

  20. Follicle Stimulating Hormone (FSH) Dosage Based on Body Weight Enhances Ovulatory Responses and Subsequent Embryo Production in Goats

    PubMed Central

    Rahman, M. R.; Rahman, M. M.; Wan Khadijah, W. E.; Abdullah, R. B.

    2014-01-01

    An experiment was conducted to evaluate the efficacy of porcine follicle stimulating hormone (pFSH) dosage based on body weight (BW) on ovarian responses of crossbred does. Thirty donor does were divided into 3 groups getting pFSH dosages of 3, 5, and 8 mg pFSH per kg BW, respectively, and were named as pFSH-3, pFSH-5 and pFSH-8, respectively. Estrus was synchronized by inserting a controlled internal drug release (CIDR) device and a single injection of prostaglandin F2? (PGF2?). The pFSH treatments were administered twice a day through 6 decreasing dosages (25, 25, 15, 15, 10, and 10% of total pFSH amount; decreasing daily). Ovarian responses were evaluated on Day 7 after CIDR removal. After CIDR removal, estrus was observed 3 times in a day and pFSH treatments were initiated at 2 days before the CIDR removal. All does in pFSH-5 and pFSH-8 showed estrus signs while half of the does in pFSH-3 showed estrus signs. No differences (p>0.05) were observed on the corpus luteum and total ovarian stimulation among the treatment groups, while total and transferable embryos were higher (p<0.05) in pFSH-5 (7.00 and 6.71) than pFSH-3 (3.00 and 2.80) and pFSH-8 (2.00 and 1.50), respectively. In conclusion, 5 mg pFSH per kg BW dosage gave a higher number of embryos than 3 and 8 mg pFSH per kg BW dosages. The results indicated that the dosage of pFSH based on BW is an important consideration for superovulation in goats. PMID:25178370

  1. Can the fall in serum FSH during coasting in IVF/ICSI predict clinical outcomes?

    PubMed

    Datta, Adrija Kumar; Zosmer, Ariel; Tozer, Amanda; Sabatini, Luca; Davis, Colin; Al-Shawaf, Talha

    2012-05-01

    This retrospective cohort study determined whether the total falls in serum FSH and oestradiol concentrations from start to end of coasting in IVF/intracytoplasmic sperm injection could predict clinical outcomes. Ninety-nine cycles, with gonadotrophin-releasing hormone-agonist down-regulation where coasting with serial serum oestradiol and FSH monitoring was adopted due to risk of severe ovarian hyperstimulation syndrome, were consecutively included. The primary clinical outcome was live-birth rate (LBR); other outcomes measured were number of oocytes retrieved and fertilization, implantation and clinical pregnancy rates. LBR for FSH fall>10 IU/l compared with 5-10 and<5 IU/l were 45.4% versus 22.0% and 25.0%, respectively. Mean serum FSH fall was similar with and without live birth (8.4 ± 6.2 versus 7.3 ± 5.0 IU/l) as were mean oestradiol and FSH concentrations on HCG administration, oestradiol fall, percentage fall in FSH/oestradiol and duration of coasting. None of the variables efficiently predicted live birth on regression analysis. The AUC of FSH fall was 0.53 at 11.0 IU/l. Basal FSH, starting and total gonadotrophin dose and duration of coasting were positively correlated with FSH fall. A potentially clinically important association between live birth and FSH fall during coasting was apparent, which requires further evaluation. The purpose of this retrospective cohort study was to determine whether the magnitude of fall in the serum FSH and oestradiol concentrations from start to end of coasting in IVF/intracytoplasmic sperm injection cycles could predict the clinical outcomes. Gonadotrophin-releasing hormone-agonist down-regulated cycles (n=99), where coasting with serial serum oestradiol and FSH monitoring was adopted due to risk of ovarian hyperstimulation, were consecutively included. Live birth was the primary clinical outcome measured; number of oocytes retrieved and fertilization, implantation and clinical pregnancy rates were the other outcomes examined. Live-birth rate tended to be high when FSH fall was >10 IU/l, compared with 5-10 IU/l and <5 IU/l, although not statistically significantly. Mean serum FSH fall were similar in live-birth and no-live-birth cycles (8.4 ± 6.2 versus 7.3 ± 5.0) as were mean oestradiol and FSH concentrations on hCG administration, oestradiol fall, percentage fall in FSH and oestradiol and duration of coasting. None of the variables efficiently predicted live birth. The area under the curve of FSH fall was 0.53. FSH fall of <11.0 IU/l was found to be more likely to predict negative outcome (specificity 84.72%) than predicting positive outcome when FSH fall was >11 IU/l (sensitivity 34.48%). Women's basal FSH, starting and total gonadotrophin dose of ovarian stimulation and duration of coasting had direct positive correlation with the magnitude of FSH fall. A potentially clinically important rise in live birth in association with greater FSH fall during coasting was apparent, which requires further evaluation. PMID:22417663

  2. Development of a flatfish-specific enzyme-linked immunosorbent assay for Fsh using a recombinant chimeric gonadotropin.

    PubMed

    Chauvigné, François; Verdura, Sara; Mazón, María José; Boj, Mónica; Zanuy, Silvia; Gómez, Ana; Cerdà, Joan

    2014-10-31

    In flatfishes with asynchronous and semicystic spermatogenesis, such as the Senegalese sole (Solea senegalensis), the specific roles of the pituitary gonadotropins during germ cell development, particularly of the follicle-stimulating hormone (Fsh), are still largely unknown in part due to the lack of homologous immunoassays for this hormone. In this study, an enzyme-linked immunosorbent assay (ELISA) for Senegalese sole Fsh was developed by generating a rabbit antiserum against a recombinant chimeric single-chain Fsh molecule (rFsh-C) produced by the yeast Pichia pastoris. The rFsh-C N- and C-termini were formed by the mature sole Fsh ? subunit (Fsh?) and the chicken glycoprotein hormone common ? subunit (CGA), respectively. Depletion of the antiserum to remove anti-CGA antibodies further enriched the sole Fsh?-specific antibodies, which were used to develop the ELISA using the rFsh-C for the standard curve. The sensitivity of the assay was 10 and 50pg/ml for Fsh measurement in plasma and pituitary, respectively, and the cross-reactivity with a homologous recombinant single-chain luteinizing hormone was 1%. The standard curve for rFsh-C paralleled those of serially diluted plasma and pituitary extracts of other flatfishes, such as the Atlantic halibut, common sole and turbot. In Senegalese sole males, the highest plasma Fsh levels were found during early spermatogenesis but declined during enhanced spermiation, as found in teleosts with cystic spermatogenesis. In pubertal males, however, the circulating Fsh levels were as high as in adult spermiating fish, but interestingly the Fsh receptor in the developing testis containing only spermatogonia was expressed in Leydig cells but not in the primordial Sertoli cells. These results indicate that a recombinant chimeric Fsh can be used to generate specific antibodies against the Fsh? subunit and to develop a highly sensitive ELISA for Fsh measurements in diverse flatfishes. PMID:25449660

  3. Immunization with FSH? fusion protein antigen prevents bone loss in a rat ovariectomy-induced osteoporosis model

    SciTech Connect

    Geng, Wenxin; Yan, Xingrong; Du, Huicong; Cui, Jihong; Li, Liwen, E-mail: liven@nwu.edu.cn; Chen, Fulin, E-mail: chenfl@nwu.edu.cn

    2013-05-03

    Highlights: •A GST-FSH fusion protein was successfully expressed in E. coli. •Immunization with GST-FSH antigen can raise high-titer anti-FSH polyclonal sera. •Anti-FSH polyclonal sera can neutralize osteoclastogenic effect of FSH in vitro. •FSH immunization can prevent bone loss in a rat osteoporosis model. -- Abstract: Osteoporosis, a metabolic bone disease, threatens postmenopausal women globally. Hormone replacement therapy (HTR), especially estrogen replacement therapy (ERT), is used widely in the clinic because it has been generally accepted that postmenopausal osteoporosis is caused by estrogen deficiency. However, hypogonadal ? and ? estrogen receptor null mice were only mildly osteopenic, and mice with either receptor deleted had normal bone mass, indicating that estrogen may not be the only mediator that induces osteoporosis. Recently, follicle-stimulating hormone (FSH), the serum concentration of which increases from the very beginning of menopause, has been found to play a key role in postmenopausal osteoporosis by promoting osteoclastogenesis. In this article, we confirmed that exogenous FSH can enhance osteoclast differentiation in vitro and that this effect can be neutralized by either an anti-FSH monoclonal antibody or anti-FSH polyclonal sera raised by immunizing animals with a recombinant GST-FSH? fusion protein antigen. Moreover, immunizing ovariectomized rats with the GST-FSH? antigen does significantly prevent trabecular bone loss and thereby enhance the bone strength, indicating that a FSH-based vaccine may be a promising therapeutic strategy to slow down bone loss in postmenopausal women.

  4. Isoforms and single nucleotide polymorphisms of the FSH receptor gene: implications for human reproduction

    Microsoft Academic Search

    M. Simoni; E. Nieschlag; J. Gromoll

    2002-01-01

    The FSH receptor shows three single nucleotide polymorphisms (SNPs), one in the promoter and two in exon 10. In addition, the FSH receptor mRNA undergoes extensive alternative splicing. While no physiological role for the SNP in the promoter and for alternative spliced isoforms has been demonstrated so far, the SNPs in exon 10 result in four discrete allelic variants characterized

  5. Synthetic peptides corresponding to human follicle-stimulating hormone (hFSH)-beta-(1-15) and hFSH-beta-(51-65) induce uptake of 45Ca++ by liposomes: evidence for calcium-conducting transmembrane channel formation

    SciTech Connect

    Grasso, P.; Santa-Coloma, T.A.; Reichert, L.E. Jr. (Department of Biochemistry, Albany Medical College, New York, NY (USA))

    1991-06-01

    We have previously described FSH receptor-mediated influx of 45Ca++ in cultured Sertoli cells from immature rats and receptor-enriched proteoliposomes via activation of voltage-sensitive and voltage-independent calcium channels. We have further shown that this effect of FSH does not require cholera toxin- or pertussis toxin-sensitive guanine nucleotide binding protein or activation of adenylate cyclase. In the present study, we have identified regions of human FSH-beta-subunit which appear to be involved in mediating calcium influx. We screened 11 overlapping peptide amides representing the entire primary structure of hFSH-beta-subunit for their effects on 45Ca++ flux in FSH receptor-enriched proteoliposomes. hFSH-beta-(1-15) and hFSH-beta-(51-65) induced uptake of 45Ca++ in a concentration-related manner. This effect of hFSH-beta-(1-15) and hFSH-beta-(51-65) was also observed in liposomes lacking incorporated FSH receptor. Reducing membrane fluidity by incubating liposomes (containing no receptor) with hFSH-beta-(1-15) or hFSH-beta-(51-65) at temperatures lower than the transition temperatures of their constituent phospholipids resulted in no significant (P greater than 0.05) difference in 45Ca++ uptake. The effectiveness of the calcium ionophore A23187, however, was abolished. Ruthenium red, a voltage-independent calcium channel antagonist, was able to completely block uptake of 45Ca++ induced by hFSH-beta-(1-15) and hFSH-beta-(51-65) whereas nifedipine, a calcium channel blocker specific for L-type voltage-sensitive calcium channels, was without effect. These results suggest that in addition to its effect on voltage-sensitive calcium channel activity, interaction of FSH with its receptor may induce formation of transmembrane aqueous channels which also facilitate influx of extracellular calcium.

  6. Single nucleotide polymorphism of FSH? gene associated with reproductive traits in Japanese flounder ( Paralichthys olivaceus)

    NASA Astrophysics Data System (ADS)

    He, Feng; Wen, Haishen; Yu, Dahui; Li, Jifang; Shi, Bao; Chen, Caifang; Zhang, Jiaren; Jin, Guoxiong; Chen, Xiaoyan; Shi, Dan; Yang, Yanping

    2010-12-01

    Follicle stimulating hormone ? (FSH?) of Japanese flounder ( Paralichthys olivaceus) plays a key role in the regulation of gonadal development. This study aimed to investigate molecular genetic characteristics of the FSH? gene and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSH? on reproductive traits in Japanese flounder. We used polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) and sequencing of the FSH? gene in 60 individuals. We identified only an SNP (T/C) in the coding region of exon3 of FSH?. The SNP (T/C) did not lead to amino acid changes at the position 340 bp of FSH? gene. Statistical analysis showed that the SNP was significantly associated with testosterone (T) level and gonadosomatic index (GSI) ( P < 0.05). Individuals with genotype TC of the SNP had significantly higher serum T levels and GSI ( P < 0.05) than that of genotype CC. Therefore, FSH? gene could be a useful molecular marker in selection for prominent reproductive trait in Japanese Flounder.

  7. Impaired fertility and FSH synthesis in gonadotrope-specific Foxl2 knockout mice.

    PubMed

    Tran, Stella; Zhou, Xiang; Lafleur, Christine; Calderon, Michael J; Ellsworth, Buffy S; Kimmins, Sarah; Boehm, Ulrich; Treier, Mathias; Boerboom, Derek; Bernard, Daniel J

    2013-03-01

    Impairments in pituitary FSH synthesis or action cause infertility. However, causes of FSH dysregulation are poorly described, in part because of our incomplete understanding of mechanisms controlling FSH synthesis. Previously, we discovered a critical role for forkhead protein L2 (FOXL2) in activin-stimulated FSH ?-subunit (Fshb) transcription in immortalized cells in vitro. Here, we tested the hypothesis that FOXL2 is required for FSH synthesis in vivo. Using a Cre/lox approach, we selectively ablated Foxl2 in murine anterior pituitary gonadotrope cells. Conditional knockout (cKO) mice developed overtly normally but were subfertile in adulthood. Testis size and spermatogenesis were significantly impaired in cKO males. cKO females exhibited reduced ovarian weight and ovulated fewer oocytes in natural estrous cycles compared with controls. In contrast, ovaries of juvenile cKO females showed normal responses to exogenous gonadotropin stimulation. Both male and female cKO mice were FSH deficient, secondary to diminished pituitary Fshb mRNA production. Basal and activin-stimulated Fshb expression was similarly impaired in Foxl2 depleted primary pituitary cultures. Collectively, these data definitively establish FOXL2 as the first identified gonadotrope-restricted transcription factor required for selective FSH synthesis in vivo. PMID:23340250

  8. LAPS-FSH: a new and effective long-acting follicle-stimulating hormone analogue for the treatment of infertility.

    PubMed

    Jung, Sunyoung; Park, Youngjin; Kim, YoungHoon; Kim, Yu Yon; Choi, Hyun-Ji; Son, Woo-Chan; Kwon, SeChang

    2014-10-01

    Although several long-acting follicle-stimulating hormone (FSH) therapies have been developed to enhance the ovarian response, a disadvantage of FSH therapy is its relatively short half-life, which requires women to receive one to two injections per day for almost 2 weeks. In the present study, we developed a novel FSH analogue by conjugating recombinant human FSH (rhFSH) and the constant region of the human immunoglobulin G4 fragment via non-peptidyl linkers. The efficacy of the FSH analogue was evaluated in vitro by cAMP level assessments, pharmacokinetic studies and a determination of ovarian weight and by comparing these findings with the results from other FSH analogues. In addition, the total number of antral and Graafian follicles was determined after 7 days of treatment with control, 6µgkg(-1) follitropin ?, 6, 12 or 42µgkg(-1) corifollitropin ? or 3, 6 or 12µgkg(-1) long acting protein/peptide discovery-follicle-stimulating hormone (LAPS-FSH). As a result, the animals treated with 12µgkg(-1) LAPS-FSH produced additional and larger healthy follicles. These data demonstrate that LAPS-FSH promotes growth and inhibits atresia of the ovarian follicle compared with other available drugs, suggesting that our new drug enhances the efficacy and duration of treatment. It is expected that our new FSH analogue will result in a higher chance of pregnancy in patients who are unresponsive to other drugs. PMID:24044514

  9. FSH? knockout mouse model: a decade ago and into the future

    Microsoft Academic Search

    T. Rajendra Kumar

    2009-01-01

    In 1997, more than 10 years ago now, we first reported the phenotypes of follicle stimulating hormone (FSH) ? null mice. Since\\u000a then, these mice have been useful for various physiological and genetic studies in reproductive biology. More recently, extra-gonadal\\u000a functions of FSH have been discovered in bone. These studies opened up exciting avenues of new research on osteoporosis in\\u000a postmenopausal

  10. Pharmacokinetics and follicular dynamics of corifollitropin alfa versus recombinant FSH during ovarian stimulation for IVF.

    PubMed

    Fauser, Bart C J M; Alper, Michael M; Ledger, William; Schoolcraft, William B; Zandvliet, Anthe; Mannaerts, Bernadette M J L

    2011-02-01

    A single injection of corifollitropin alfa can replace seven daily injections of recombinant FSH (rFSH) using a gonadotrophin-releasing hormone antagonist protocol in ovarian stimulation prior to IVF or intracytoplasmic sperm injection. This double-blind randomized controlled trial assessed the pharmacokinetics and pharmacodynamics of 150 ?g corifollitropin alfa versus daily 200 IU rFSH in 1509 patients. Comparative analyses were performed on serum concentrations of FSH immunoreactivity (pharmacokinetics), and the number and size of growing follicles, and inhibin B and oestradiol concentrations as biomarkers of ovarian response (pharmacodynamics). The rate of follicular development was similar in both treatment groups. By stimulation day 8, 33% of patients treated with corifollitropin alfa reached the criterion for human chorionic gonadotrophin (HCG) injection. The number of follicles ?11 mm was slightly higher after corifollitropin alfa compared with daily rFSH at stimulation day 8 (difference, 1.2; 95% confidence interval (Cl) 0.5-1.8; P < 0.01) and on the day of HCG injection (difference, 2.1; 95% Cl 1.4-2.8; P < 0.01). The rise of inhibin B and oestradiol concentrations was similar in both treatment groups. Although the pharmacokinetics of corifollitropin alfa and rFSH are quite different their pharmacodynamic profiles at the dosages used are similar. PMID:21575846

  11. Redirecting intracellular trafficking and the secretion pattern of FSH dramatically enhances ovarian function in mice

    PubMed Central

    Wang, Huizhen; Larson, Melissa; Jablonka-Shariff, Albina; Pearl, Christopher A.; Miller, William L.; Conn, P. Michael; Boime, Irving; Kumar, T. Rajendra

    2014-01-01

    FSH and luteinizing hormone (LH) are secreted constitutively or in pulses, respectively, from pituitary gonadotropes in many vertebrates, and regulate ovarian function. The molecular basis for this evolutionarily conserved gonadotropin-specific secretion pattern is not understood. Here, we show that the carboxyterminal heptapeptide in LH is a gonadotropin-sorting determinant in vivo that directs pulsatile secretion. FSH containing this heptapeptide enters the regulated pathway in gonadotropes of transgenic mice, and is released in response to gonadotropin-releasing hormone, similar to LH. FSH released from the LH secretory pathway rescued ovarian defects in Fshb-null mice as efficiently as constitutively secreted FSH. Interestingly, the rerouted FSH enhanced ovarian follicle survival, caused a dramatic increase in number of ovulations, and prolonged female reproductive lifespan. Furthermore, the rerouted FSH vastly improved the in vivo fertilization competency of eggs, their subsequent development in vitro and when transplanted, the ability to produce offspring. Our study demonstrates the feasibility to fine-tune the target tissue responses by modifying the intracellular trafficking and secretory fate of a pituitary trophic hormone. The approach to interconvert the secretory fate of proteins in vivo has pathophysiological significance, and could explain the etiology of several hormone hyperstimulation and resistance syndromes. PMID:24706813

  12. Expression of FSH receptor in the hamster ovary during perinatal development.

    PubMed

    Chakraborty, Prabuddha; Roy, Shyamal K

    2015-01-15

    FSH plays an important role in ovarian follicular development, and it functions via the G-protein coupled FSH receptor. The objectives of the present study were to determine if full-length FSHR mRNA and corresponding protein were expressed in fetal through postnatal hamster ovaries to explain the FSH-induced primordial follicle formation, and if FSH or estrogen (E) would affect the expression. A full-length and two alternately spliced FSHR transcripts were expressed from E14 through P20. The level of the full-length FSHR mRNA increased markedly through P7 before stabilizing at a lower level with the formation and activation of primordial follicles. A predicted 87?kDa FSHR protein band was detected in fetal through P4 ovaries, but additional bands appeared as ovary developed. FSHR immunosignal was present in undifferentiated somatic cells and oocytes in early postnatal ovaries, but was granulosa cells specific after follicles formed. Both eCG and E significantly up-regulated full-length FSHR mRNA levels. Therefore, FSHR is expressed in the hamster ovary from the fetal life to account for FSH-induced primordial follicle formation and cAMP production. Further, FSH or E regulates the receptor expression. PMID:25462586

  13. Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

    PubMed Central

    2012-01-01

    Background To compare the outcome of IVF cycles in women receiving controlled ovarian stimulation with recFSH or recFSH plus low dose hCG. Methods A retrospective case control study, performed at a private practice affiliated with an academic institute. Patients were infertile women who were treated with IVF/ICSI and controlled ovarian stimulation in a long GnRH agonist protocol using either low dose hCG in addition to recFSH [N?=?88] or recFSH alone [N?=?99]. Primary outcomes were mean FSH dose, number of mature eggs, number of fertilized eggs, and serum levels of estradiol. Secondary outcomes were endometrial thickness, cycle cancellations and pregnancy rates. Results A significant increase in number of mature and fertilized eggs was observed in women over 40?years of age using low dose hCG in addition to recFSH. The estradiol level was significantly higher on the day of hCG administration and the serum level of FSH on cycle day 7 and on the day of hCG administration were lower. Conclusion Addition of low dose hCG to recFSH compared with recFSH alone significantly modified cycle characteristics in patients >/= 40?years and could be of potential benefit for IVF cycles in older infertile women. PMID:22866896

  14. Pubertal Onset in Girls is Strongly Influenced by Genetic Variation Affecting FSH Action

    PubMed Central

    Hagen, Casper P.; Sørensen, Kaspar; Aksglaede, Lise; Mouritsen, Annette; Mieritz, Mikkel G.; Tinggaard, Jeanette; Wohlfart-Veje, Christine; Petersen, Jørgen Holm; Main, Katharina M.; Meyts, Ewa Rajpert-De; Almstrup, Kristian; Juul, Anders

    2014-01-01

    Age at pubertal onset varies substantially in healthy girls. Although genetic factors are responsible for more than half of the phenotypic variation, only a small part has been attributed to specific genetic polymorphisms identified so far. Follicle-stimulating hormone (FSH) stimulates ovarian follicle maturation and estradiol synthesis which is responsible for breast development. We assessed the effect of three polymorphisms influencing FSH action on age at breast deveopment in a population-based cohort of 964 healthy girls. Girls homozygous for FSHR -29AA (reduced FSH receptor expression) entered puberty 7.4 (2.5–12.4) months later than carriers of the common variants FSHR -29GG+GA, p = 0.003. To our knowledge, this is the strongest genetic effect on age at pubertal onset in girls published to date. PMID:25231187

  15. Comparative incidence of ovarian hyperstimulation syndrome following ovarian stimulation with corifollitropin alfa or recombinant FSH.

    PubMed

    Tarlatzis, Basil C; Griesinger, Georg; Leader, Arthur; Rombauts, Luk; Ijzerman-Boon, Pieta C; Mannaerts, Bernadette M J L

    2012-04-01

    Corifollitropin alfa is a novel recombinant gonadotrophin with sustained follicle-stimulating activity. A single injection can replace seven daily injections of recombinant follicle-stimulating hormone (rFSH) during the first week of ovarian stimulation. All cases of ovarian hyperstimulation syndrome (OHSS) with corifollitropin alfa intervention in a gonadotrophin-releasing hormone antagonist protocol have been assessed in three large trials: Engage, Ensure and Trust. Overall, 1705 patients received corifollitropin alfa and 5.6% experienced mild, moderate or severe OHSS. In the randomized controlled trials, Engage and Ensure, the pooled incidence of OHSS with corifollitropin alfa was 6.9% (71/1023 patients) compared with 6.0% (53/880 patients) in the rFSH group. Adjusted for trial, the odds ratio for OHSS was 1.18 (95% CI 0.81-1.71) indicating that the risk of OHSS for corifollitropin alfa was similar to that for rFSH. The incidence of mild, moderate and severe OHSS was 3.0%, 2.2% and 1.8%, respectively, with corifollitropin alfa, with 1.9% requiring hospitalization, and 3.5%, 1.3% and 1.3%, respectively, in the rFSH arms, with 0.9% requiring hospitalization. Despite a higher ovarian response with corifollitropin alfa compared with rFSH for the first 7days of ovarian stimulation, the incidence of OHSS was similar. Corifollitropin alfa is a new agent used in ovarian stimulation treatment for IVF fertilization. One injection of corifollitropin alfa can replace seven injections of recombinant FSH (rFSH). In three studies of corifollitropin alfa treatment, we assessed all cases of ovarian hyperstimulation syndrome (OHSS), a potentially serious complication of ovarian stimulation treatment. Overall, 5.6% of the patients (95/1701) experienced OHSS. Two of the trials compared corifollitropin alfa versus rFSH. Because OHSS is relatively rare, we pooled the results of these trials to give a more reliable estimate of the incidence of OHSS. In the pooled analysis, 6.9% (71/1023) of patients receiving corifollitropin alfa had signs or symptoms of OHSS, compared with 6.0% in the rFSH group (53/880). The risk of OHSS with corifollitropin alfa treatment was similar to the risk of OHSS in patients who received rFSH: the incidence of mild, moderate and severe OHSS was 3.0%, 2.2% and 1.8%, respectively, in patients in the corifollitropin alfa treatment groups, with 1.9% requiring hospitalisation, and 3.5%, 1.3% and 1.3%, respectively, in patients in the rFSH treatment groups, with 0.9% requiring hospitalization. Although the ovaries respond more to corifollitropin alfa than to rFSH for the first 7days of ovarian stimulation, neither treatment regimen was significantly more likely to cause OHSS. PMID:22386594

  16. Fsh and Lh direct conserved and specific pathways during flatfish semicystic spermatogenesis.

    PubMed

    Chauvigné, François; Zapater, Cinta; Crespo, Diego; Planas, Josep V; Cerdà, Joan

    2014-10-01

    The current view of the control of spermatogenesis by Fsh and Lh in non-mammalian vertebrates is largely based on studies carried out in teleosts with cystic and cyclic spermatogenesis. Much less is known concerning the specific actions of gonadotropins during semicystic germ cell development, a type of spermatogenesis in which germ cells are released into the tubular lumen where they transform into spermatozoa. In this study, using homologous gonadotropins and a candidate gene approach, for which the genes' testicular cell-type-specific expression was established, we investigated the regulatory effects of Fsh and Lh on gene expression during spermatogenesis in Senegalese sole (Solea senegalensis), a flatfish with asynchronous and semicystic germ cell development. During early spermatogenesis, Fsh and Lh upregulated steroidogenesis-related genes and nuclear steroid receptors, expressed in both somatic and germ cells, through steroid-dependent pathways, although Lh preferentially stimulated the expression of downstream genes involved in androgen and progestin syntheses. In addition, Lh specifically promoted the expression of spermatid-specific genes encoding spermatozoan flagellar proteins through direct interaction with the Lh receptor in these cells. Interestingly, at this spermatogenic stage, Fsh primarily regulated genes encoding Sertoli cell growth factors with potentially antagonistic effects on germ cell proliferation and differentiation through steroid mediation. During late spermatogenesis, fewer genes were regulated by Fsh or Lh, which was associated with a translational and posttranslational downregulation of the Fsh receptor in different testicular compartments. These results reveal that conserved and specialized gonadotropic pathways regulate semicystic spermatogenesis in flatfish, which may spatially adjust cell germ development to maintain a continuous reservoir of spermatids in the testis. PMID:25024405

  17. Impaired FSH? Expression in the Pituitaries of Foxl2 Mutant Animals

    PubMed Central

    Justice, Nicholas J.; Blount, Amy L.; Pelosi, Emanuele; Schlessinger, David; Vale, Wylie

    2011-01-01

    Forkhead box L2 (FoxL2) is required for ovarian development and differentiation. FoxL2 is also expressed in the pituitary where it has been implicated in the development and regulation of gonadotropes, which secrete LH and FSH, the endocrine signals that regulate folliculogenesis in the ovary and spermatogenesis in the testis. Here, we show that FoxL2 is not required for the specification of gonadotropes; the pituitaries of Foxl2 mutant mice contain normal numbers of gonadotropes that express glycoprotein ? subunit and LH?. Whereas the specification of gonadotropes and all other hormonal cell types is normal in the pituitaries of Foxl2 mutant animals, FSH? levels are severely impaired in both male and female animals, suggesting that FoxL2 is required for normal Fshb expression. The size of the pituitary is reduced in proportion to the smaller body size of Foxl2 mutants, with a concomitant increase in the pituitary cellular density. In primary pituitary cultures, activin induces FSH secretion and Fshb mRNA expression in cells from wild-type mice. In cells from Foxl2 mutant mice, however, FSH secretion is not detected, and activin is unable to drive Fshb expression, suggesting that the mechanism of activin-dependent activation of Fshb transcription is impaired. However, a small number of gonadotropes in the ventromedial region of the pituitaries from Foxl2 mutant mice maintain FSH? expression, suggesting that a FoxL2- and activin-independent mechanism can drive Fshb transcription. These data indicate that, in addition to its role in the ovary, FoxL2 function in the pituitary is required for normal expression of FSH. PMID:21700720

  18. GnRH Increases c-Fos Half-Life Contributing to Higher FSH? Induction

    PubMed Central

    Reddy, Gaddameedi R.; Xie, Changchuan; Lindaman, Lacey L.

    2013-01-01

    GnRH is a potent hypothalamic regulator of gonadotropin hormones, LH and FSH, which are both expressed within the pituitary gonadotrope and are necessary for the stimulation of gametogenesis and steroidogenesis in the gonads. Differential regulation of LH and FSH, which is essential for reproductive fitness, is achieved, in part, through the varying of GnRH pulse frequency. However, the mechanism controlling the increase in FSH during the periods of low GnRH has not been elucidated. Here, we uncover another level of regulation by GnRH that contributes to differential expression of the gonadotropins and may play an important role for the generation of the secondary rise of FSH that stimulates folliculogenesis. GnRH stimulates LH? and FSH? subunit transcription via induction of the immediate early genes, Egr1 and c-Fos, respectively. Here, we determined that GnRH induces rapidly both Egr1 and c-Fos, but specifically decreases the rate of c-Fos degradation. In particular, GnRH modulates the rate of c-Fos protein turnover by inducing c-Fos phosphorylation through the ERK1/2 pathway. This extends the half-life of c-Fos, which is normally rapidly degraded. Confirming the role of phosphorylation in promoting increased protein activity, we show that a c-Fos mutant that cannot be phosphorylated by GnRH induces lower expression of the FHS? promoter than wild-type c-Fos. Our studies expand upon the role of GnRH in the regulation of gonadotropin gene expression by highlighting the role of c-Fos posttranslational modification that may cause higher levels of FSH during the time of low GnRH pulse frequency to stimulate follicular growth. PMID:23275456

  19. Molecular Analysis of a Mutated FSH Receptor Detected in a Patient with Spontaneous Ovarian Hyperstimulation Syndrome

    PubMed Central

    Uchida, Sayaka; Uchida, Hiroshi; Maruyama, Tetsuo; Kajitani, Takashi; Oda, Hideyuki; Miyazaki, Kaoru; Kagami, Maki; Yoshimura, Yasunori

    2013-01-01

    Spontaneous ovarian hyperstimulation syndrome (sOHSS) is a rare event that may result from a FSH-producing pituitary adenoma (FSHoma), activating mutations of the FSH receptor (FSHR), and cross-reactivity of the FSHR to elevated hCG and TSH in the setting of pregnancy or hypothyroidism. The objective of this study was to investigate whether an aberrant FSHR was present in a woman with sOHSS and a non-surgically diagnosed FSHoma whose serum FSH levels and FSH bioactivity were nearly normal. Sequencing of the patient’s FSHR gene revealed a heterozygous novel missense mutation c. 1536G>A resulting in an amino acid substitution M512I. We asked whether this mutant FSHR affected FSHR-mediated signaling pathways involving cAMP/protein kinase A (PKA), phosphatidylinositol-3 kinase (PI3K)/protein kinase B (AKT) and v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog kinase (SRC)/ p42/p44 extracellular signal-regulated protein kinases (ERK1/2). Thus, 293T cells expressing wild-type (FSHRwt), the mutant FSHR (FSHRmt), or both (FSHRwt/mt) were treated with FSH and subjected to measurements of intracellular cAMP, cAMP-induced CRE (cAMP response element)-mediated luciferase assays and immunoblot analyses of phosphorylated PI3K and ERK1/2. There were no differences in luciferase activities or phosphorylation levels of ERK1/2 among FSHRwt, FSHRmt cells and FSHwt/mt cells. However, FSHRmt cells showed a significant reduction in both cAMP production and PI3K phosphorylation levels with unchanged phosphorylation of ERK1/2 upon FSH stimulation in comparison to FSHwt cells. Also, FSH treatment did not provoke PI3K phosphorylation in FSHwt/mt cells. These results indicate that the novel missense M512I FSHR mutation identified herein did not participate in hyperactivation of FSHR-mediated signaling pathways but rather in hypoactivation of the FSH-mediated PI3K/AKT pathway. Thus, this study demonstrates a new functional property of this novel mutatnt FSHR, which, however, might not be involved in the pathogenesis of sOHSS in this FSHoma patient. PMID:24058690

  20. Successful use of aromatase inhibitor letrozole in NOA with an elevated FSH level: a case report.

    PubMed

    Zhao, D; Pan, L; Zhang, F; Pan, F; Ma, J; Zhang, X; Liu, Y

    2014-05-01

    Aromatase inhibitors inhibit the conversion of testosterone to oestrogens and could reduce serum oestradiol concentrations. Letrozole is one of aromatase inhibitors frequently used in treatment of men with oligospermia. We present the case of an infertile man with small testes and an elevated FSH level, which was diagnosed as NOA, hypospermatogenesis proven by testicular biopsy. After taking letrozole for 3 months, semen analyses by computer-aided sperm analysis present that this man had normal spermatogenesis. This is the first case report of the activation of spermatogenesis, in man who was NOA with elevated FSH level, resulting from the use of the one of aromatase inhibitors. PMID:23803162

  1. Effects of novel single nucleotide polymorphisms of the FSH beta-subunit gene on semen quality and fertility in bulls

    Microsoft Academic Search

    Lisheng Dai; Zhihui Zhao; Ruifeng Zhao; Shuqi Xiao; Hao Jiang; Xupeng Yue; Xichun Li; Yan Gao; Jifeng Liu; Jiabao Zhang

    2009-01-01

    The follicle-stimulating hormone (FSH) acts on the Sertoli cells in the seminiferous tubules of the testis and regulates spermatogenesis up to the secondary spermatocyte stage. This study aimed to investigate molecular genetic characteristics of the bovine FSH ?-subunit gene (FSHB) and elucidate the effects of single nucleotide polymorphisms (SNPs) of FSHB on the quality of fresh and frozen semen and

  2. TESTICULE ET SPERMATOGENSE EFFECTS OF FSH, LH AND PROLACTIN ON THE TESTIS AND ACCESSORY

    E-print Network

    Paris-Sud XI, Université de

    TESTICULE ET SPERMATOGENÈSE EFFECTS OF FSH, LH AND PROLACTIN ON THE TESTIS AND ACCESSORY ORGANS. Frequent measurements of plasma LH and testosterone, testis size, skin colour and behaviour - the latter AND SUPERIOR CERVICAL GANGLIO- NECTOMY ON THE TESTIS OF THE VOLE, MICROTUS AGRESTIS. H. M. CHARLTON, C. A

  3. Effects of FSH and testosterone on Sertoli cells and spermatocytes from rat testis

    E-print Network

    Paris-Sud XI, Université de

    Effects of FSH and testosterone on Sertoli cells and spermatocytes from rat testis F. F. G biochemical parameters have been studied in isolated Sertoli cells and germinal cells from rat testis the same testis tissue. Isolated Sertoli cells when incubated with 3H-leucine secreted in addition

  4. Serum FSH Levels in Coasting Programmes on the hCG Day and Their Clinical Outcomes in IVF ± ICSI Cycles

    PubMed Central

    Vitthala, Srisailesh; Bouaziz, Jerome; Tozer, Amanda; Zosmer, Ariel; Al-Shawaf, Talha

    2012-01-01

    Introduction. Coasting is the most commonly used strategy in prevention of severe OHSS. Serum FSH levels measurements during coasting may aid in optimizing the duration of coasting. Objective(s). To study live birth rates (LBRs), clinical pregnancy rates (CPRs), and optimal duration of coasting based on serum FSH levels on the hCG day. Materials and Methods. It is a retrospective study performed between 2005 and 2008 at Barts and The London Centre for Reproductive Medicine, NHS Trust, London, UK, on 349-coasted women undergoing controlled ovarian stimulation (COS) for IVF ± ICSI. The serum FSH level measurements on the hCG day during coasting programme were analysed to predict the LBR and CPR. Result(s). LBR and CPR were significantly higher when the FSH levels on the hCG day were >2.5?IU/L (LBR: 32.5%, P = 0.045 and CPR: 36.9%, P = 0.027) compared to FSH <2.5?IU/L. The optimal FSH cut-off level for LBR and CPR is 5.6?IU/L on the hCG day. The optimal cutoff for coasting is 4 days. Conclusion(s). Coasting may be continued as long as either serum FSH level is > 2.5?IU/L on the hCG day without compromising the LBR and CPR or to maximum of 4 days. PMID:22518127

  5. Polymorphism of follicle stimulating hormone beta (FSH?) subunit gene and its association with litter traits in giant panda.

    PubMed

    Huang, Xiaoyu; Li, Desheng; Wang, Jiwen; Huang, Yan; Han, Chunchun; Zhang, Guiquan; Huang, Zhi; Wu, Honglin; Wei, Ming; Wang, Guosong; Hu, Haiping; Deng, Tao; He, Tao; Zhou, Yingming; Song, Shixian; Luo, Bo; Zhang, Heming

    2013-11-01

    The different SSCP patterns of the follicle stimulating hormone beta (FSH?) gene amplified by three pairs of primers were sequenced. Comparisons among the three nucleotide sequences of three genotypes indicated that three base substitutions (A213T, A91G, and A89C) were detected in FSH? gene, which A213T substitution led to one amino acids mutation (Lys > Met), and the other two substitutions were synonymous mutations. The AA, AB and BB genotypes patterns obtained by FSH? primer1 had evident relation with the litter traits, but the SSCP genotypes patterns obtained by FSH? primer2 and primer3 had no evident relation with the litter traits in giant panda. The giant panda with AA and AB genotype had the largest litter size and multiparity rate compared with the BB genotypes (P < 0.05). We speculated that the giant pandas with the A allele have better litter traits than those with the B allele. PMID:24057246

  6. Effects of leptin on FSH cells in the pituitary gland of Podarcis siculus.

    PubMed

    Ferrandino, Ida; Monaco, Antonio; Grimaldi, Maria Consiglio

    2015-03-01

    Leptin is the hormone synthesised by adipocytes, which plays an important role in regulating appetite and metabolism. In mammals, this pleiotropic hormone also plays a key role in controlling gonadotropin secretion by stimulatory hypothalamic and pituitary actions. However, little is known about leptin in lower vertebrates and particularly few studies are available on reptiles. In the present work, we analysed the action of recombinant human leptin on FSH cells in the pituitary gland of Podarcis siculus female lizards exposed to four different concentrations of the hormone. FSH cells showed a dose-dependent reaction. The data are indicative of the role played by leptin in modulating the cellular activity of such cells in the pituitary gland of P. siculus, similar to what was already reported in mammals. A functional receptor is evidently able to respond to leptin in this lizard, but further comparative studies are needed to understand the role of this hormone in ectothermic vertebrates. PMID:25617161

  7. Evaluation of two oestrus synchronization regimens in eFSH-treated donor mares

    Microsoft Academic Search

    Tal Raz; Sylvia D. Carley; Jodyne M. Green; Claire E. Card

    2011-01-01

    Reliable methods for regulating oestrus and superovulation in equine embryo transfer (ET) programs are desirable. The objective in this study was to compare two oestrus synchronization methods combined with equine follicle-stimulating hormone (eFSH) treatment in an ET program. In the progesterone and estradiol-17? (P&E) group, mares (n=12) were given progesterone and estradiol-17?, daily for 10days, followed by prostaglandin (PG)F2? on

  8. Human Menopausal Gonadotropin versus Recombinant FSH in Polycystic Ovary Syndrome Patients Undergoing In Vitro Fertilization

    PubMed Central

    Figen Turkcapar, Ayse; Seckin, Berna; Onalan, Gogsen; Ozdener, Tulin; Batioglu, Sertac

    2013-01-01

    Background: We aimed to compare human menopausal gonadotropin (hMG) and recombinant follicle-stimulating hormone (r FSH) with respect to clinical outcomes and the development of ovarian hyperstimulation syndrome (OHSS) for patients with polycystic ovary syndrome (PCOS) treated with in vitro fertilization (IVF). Materials and Methods: This prospective randomized controlled trial included a total of 80 women with PCOS. Of these, 38 were randomized to receive treatment with hMG and 42 with rFSH using a long gonadotropin releasing hormone (GnRH) analogue protocol. Outcome measures were cycle characteristics, pregnancy rates, the need for coasting, and OHSS rates. Results: In the hMG group we observed a significantly lower peak estradiol (E2) level (p=0.02), fewer intermediate-sized follicles (p=0.001), lower number of oocytes retrieved (p=0.002) and metaphase II (MII) oocytes (p=0.003). However, there were no significant differences between the groups in the number of fertilized oocytes, fertilization rates, top quality embryo counts, and the number of transferred embryos. There was no difference in pregnancy rates between the groups. OHSS occurred in 11.9% of the rFSH group patients, whereas no OHSS developed in the hMG group. Coasting requirements were lower in the hMG group (19.2% vs. 48.9%, p=0.013). Conclusion: Ovarian stimulation with hMG and rFSH provides similar clinical pregnancy rates in PCOS patients treated with a long GnRH agonist protocol in IVF cycles. hMG stimulation appears to be associated with a lower rate of OHSS and decreased coasting requirements (Registration Number: NCT01365936). PMID:24520446

  9. Importance of Inhibin B in the Regulation of FSH Secretion in the Human Male

    Microsoft Academic Search

    FRANCES J. HAYES; NELLY PITTELOUD; PAUL A. BOEPPLE

    Regulation of FSH secretion in the male involves a complex balance between stimulation by GnRH from the hypothala- mus, inhibitory feedback by sex steroids (T and E2) and in- hibin B (Inh B) from the gonads, and autocrine\\/paracrine mod- ulation by activin and follistatin within the pituitary. The aim of the present study was to delineate the feedback control of

  10. Multiple birth induction with FSH-LH in synchronized beef cattle 

    E-print Network

    Burguera, Mario Heberto

    1973-01-01

    administration of 6 ? methyl 17 ? acetoxy progesterone Aust. J. Exp. Agric. Anim. Hush, , 3:78. Scanlon, P. , J ~ Sveenan, I' Gordon. 1968 Hormonal induction of superovulation in cattle. Segal, S. J. , H. Croxatto. 1967. Single administration of hormones...MULTIPLE BIRTH INDUCTION WITH FSH LH IN SYNCHRONIZED BEEF CATTLE A Thesis by Mario Heberto Burguera submitted to the graduate College of Texas ARM University in partial fulfillment of the requirement for the degree of MASTER OF SCIENCE May...

  11. Identification of immunoreactive FSH and LH cells in the cichlid fish Cichlasoma dimerus during the ontogeny and sexual differentiation

    Microsoft Academic Search

    Matías Pandolfi; Fabiana L. Lo Nostro; Akio Shimizu; Andrea G. Pozzi; Fernando J. Meijide; Graciela Rey Vazquez; M. Cristina Maggese

    2006-01-01

    Follicle-stimulating hormone (FSH) and luteinizing hormone (LH) expressing cells were detected in pituitary, brain and ovary\\u000a of the Perciform cichlid fish Cichlasoma dimerus. This detection was carried out by immunohistochemistry (IHC) and Western blot techniques using antisera of the Cyprinodontiform\\u000a Fundulus heteroclitus raised against the conservative region of the teleost ?FSH and the ?LH subunits. The estimated molecular weights were

  12. Effect of Serotoninergic System on FSH Secretion in Male and Female Rats: Evidence for Stimulatory and Inhibitory Actions

    Microsoft Academic Search

    Sergio N. Justo; Graciela L. Rossano; Berta Szwarcfarb; Modesto C. Rubio; Jaime A. Moguilevsky

    1989-01-01

    The present experiments were designed to assess the effect of the serotoninergic system on FSH secretion in prepubertal (16-, 18-, 20-, 26- and 30-day-old) and adult (60-day-old) male and female rats. The intraperitoneal administration of 5-hydroxytryptophan (5-HTP), a serotonin (5-HT) precursor, induced a significant increase of FSH levels in male rats at 16, 26, 30 and 60 days of age

  13. Age-dependent changes in the in-vitro response of a pig Sertoli cell-enriched population to FSH

    Microsoft Academic Search

    C. Monet-Kuntz; I. Fontaine

    1989-01-01

    Summary. The response of pig Sertoli cell-enriched cultures to FSH was investigated by measuring plasminogen activator (PA) secretion in culture, throughout the non- pubertal and prepubertal periods. Sertoli cell-enriched populations could be isolated from birth until a testicular weight of 56 g. FSH elicited a dose-dependent increase in PA secretion by pig Sertoli cell-enriched cultures. The ED50 was minimal for

  14. The effect of Ramadan fasting on LH, FSH, oestrogen, progesterone and leptin in pregnant women.

    PubMed

    Khoshdel, A; Kheiri, S; Hashemi-Dehkordi, E; Nasiri, J; Shabanian-Borujeni, S; Saedi, E

    2014-10-01

    Many pregnant Muslim women fast during Ramadan. Leptin has an important role in the reproductive system and hormones. In this study, FSH, LH, oestrogen, progesterone and leptin were measured in the first, second and fourth week of Ramadan and the second week post-Ramadan, in 30 fasting pregnant women. Data were analysed using repeated measures ANOVA by SPSS. The weight and BMI did not change during the study. A significant change in FSH, oestrogen, progesterone and leptin was observed (p < 0.05). The lowest value of FSH was in the second week of Ramadan. Progesterone increased at the end of Ramadan and the second week after. Oestrogen increased significantly during Ramadan and decreased after Ramadan. A decreasing trend was seen in LH during the Ramadan and 2 weeks after (p < 0.1). Leptin decreased significantly 2 weeks after Ramadan. We found poor weight gain and hypoleptinaemia in pregnant fasted women during the study. Food restriction in pregnant fasted women during Ramadan may induce poor weight gain during pregnancy. These data confirm that Ramadan fasting by pregnant women may have potential risks during pregnancy. We recommend further study to evaluate long-term effects of Ramadan fasting during pregnancy in different countries with different food habits and traditions, to obtain reliable and documented data. PMID:24914688

  15. GnRH Pulse Frequency-dependent Differential Regulation of LH and FSH Gene Expression

    PubMed Central

    Thompson, Iain R.; Kaiser, Ursula B.

    2013-01-01

    The pituitary gonadotropin hormones, FSH and LH, are essential for fertility. Containing an identical ?-subunit (CGA), they are comprised of unique ?-subunits, FSH? and LH?, respectively. These two hormones are regulated by the hypothalamic decapeptide, GnRH, which is released in a pulsatile manner from GnRH neurons located in the hypothalamus. Varying frequencies of pulsatile GnRH stimulate distinct signaling pathways and transcriptional machinery after binding to the receptor, GnRHR, on the cell surface of anterior pituitary gonadotropes. This ligand-receptor binding and activation orchestrates the synthesis and release of FSH and LH, in synergy with other effectors of gonadotropin production, such as activin, inhibin and steroids. Current research efforts aim to discover the mechanisms responsible for the decoding of the GnRH pulse signal by the gonadotrope. Modulating the response to GnRH has the potential to lead to new therapies for patients with altered gonadotropin secretion, such as those with hypothalamic amenorrhea or polycystic ovarian syndrome. PMID:24056171

  16. Corifollitropin alfa compared to daily FSH in controlled ovarian stimulation for in vitro fertilization: a meta-analysis.

    PubMed

    Fensore, Stefania; Di Marzio, Marco; Tiboni, Gian Mario

    2015-01-01

    The present study offers a meta-analysis of published randomized controlled trials (RCTs) evaluating the outcomes of in vitro fertilization (IVF) cycles using corifollitropin alfa for controlled ovarian stimulation (COS) in comparison with daily recombinant FSH (rFSH). The study examined seven RCTs including 2138 patients receiving corifollitropin alfa and 1788 women receiving daily rFSH for COS. As a novel aspect, this meta-analysis included two specific subpopulations of IVF patients, i.e. egg donors and poor responders. There were no significant differences between corifollitropin alfa and rFSH with respect to the majority of the clinical parameters considered, and comparable were the outcomes in terms of live birth rate, ongoing pregnancy rate, and clinical pregnancy rate. Women receiving corifollitropin alfa had a significantly higher number of metaphase II oocytes at ovum pick-up, and number of formed embryos, in comparison to rFSH. The risk of cycle cancellation due to overstimulation was significantly higher in the corifollitropin alfa group. Ovarian hyperstimulation syndrome (OHSS) incidence was statistically comparable between patients receiving long lasting or daily rFSH. Nevertheless, in view of the fact that corifollitropin alfa resulted in a higher number of metaphase II oocytes collected and a higher number of cycles cancelled due to overstimulation, corifollitropin alfa should be cautiously considered in women with the potential of being hyper responders. PMID:26036214

  17. Molecular cloning of follicle-stimulating hormone (FSH)-beta subunit cDNA from duck pituitary.

    PubMed

    Shen, San-Tai; Cheng, Yi-Sheng; Shen, Tzu-Yun; Yu, John Yuh-Lin

    2006-09-15

    We have cloned FSH-beta cDNA from duck pituitary gland by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA end (RACE) methods. The cloned duck FSH-beta cDNA contains 1909-bp nucleotides including 396-bp of open-reading frame and 1491-bp of 3'-untranslational region. The open-reading frame encodes a 131-amino acid protein with a putative 20-amino acid signal peptide and a putative 111-amino acid mature protein. The deduced amino acid sequence shows a remarkable similarity (94-98%) to those of other avian FSH-beta subunits; while it exhibits lower similarities with those of turtles (82-84%), mammals (63-71%), and amphibians (53-57%). The structural model analysis of duck FSH suggests that the cysteine-knot and beta-strands for maintaining the specific structural frame, and the "seat-belt" loop for specific binding to FSH receptor have been conserved in tetrapodian FSH-betas. PMID:16674957

  18. PKC? and ? Possibly Mediate FSH-Induced Mouse Oocyte Maturation via NOX-ROS-TACE Cascade Signaling Pathway

    PubMed Central

    Ran, Hao; Feng, Lizhao; Yan, Hao; Mu, Xinyi; Han, Yingying; Liu, Wei; Xia, Guoliang; Wang, Chao

    2014-01-01

    In mammals, gonadotropins stimulate oocyte maturation via the epidermal growth factor (EGF) network, and the protein kinase C (PKC) signaling pathway mediates this process. Tumor necrosis factor-? converting enzyme (TACE) is an important protein responding to PKC activation. However, the detailed signaling cascade between PKC and TACE in follicle-stimulating hormone (FSH)-induced oocyte maturation in vitro remains unclear. In this study, we found that rottlerin (mallotoxin, MTX), the inhibitor of PKC ? and ?, blocked FSH-induced maturation of mouse cumulus-oocyte complexes (COCs) in vitro. We further clarified the relationship between two molecules downstream of PKC ? and ? and TACE in COCs: nicotinamide adenine dinucleotide phosphate (NADPH) oxidase (NOX) and its products, reactive oxygen species (ROS). We proved that the respective inhibitors of NOX, ROS and TACE could block FSH-stimulated oocyte maturation dose-dependently, but these inhibitory effects could be reversed partially by amphiregulin (Areg), an EGF family member. Notably, inhibition of PKC ? and ? prevented FSH-induced translocation of two cytosolic components of NOX, p47phox and p67phox, to the plasma membrane in cumulus cells. Moreover, FSH-induced TACE activity in cumulus cells was decreased markedly by inhibition of NOX and ROS. In conclusion, PKC ? and ? possibly mediate FSH-induced meiotic resumption in mouse COCs via NOX-ROS-TACE signaling pathway. PMID:25350560

  19. BERKELEY PAR LABBERKELEY PAR LAB Where we ended up

    E-print Network

    California at Berkeley, University of

    LAB Par Lab Timeline 2 Initial Meetings "Berkeley View" Techreport Win UPCRC Competition UPCRC PhaseBERKELEY PAR LABBERKELEY PAR LAB Par Lab: Where we ended up Krste Asanovic, Ras Bodik, Jim Demmel, David Wessel, and Kathy Yelick UC Berkeley Par Lab End-of-Project Party May 30, 2013 #12;BERKELEY PAR

  20. Evaluation of two oestrus synchronization regimens in eFSH-treated donor mares.

    PubMed

    Raz, Tal; Carley, Sylvia D; Green, Jodyne M; Card, Claire E

    2011-04-01

    Reliable methods for regulating oestrus and superovulation in equine embryo transfer (ET) programs are desirable. The objective in this study was to compare two oestrus synchronization methods combined with equine follicle-stimulating hormone (eFSH) treatment in an ET program. In the progesterone and estradiol-17? (P&E) group, mares (n=12) were given progesterone and estradiol-17?, daily for 10 days, followed by prostaglandin (PG)F(2?) on the last day. In the PG group, mares (n=12) were given PGF(2?) 5 days post-ovulation. In both groups donor mares were allocated to eFSH therapy, and were subsequently bred. Embryo recovery and transfer were performed routinely. The interval to ovulation (mean ± SEM, range) was not statistically different between donor mares in the P&E group (10.2±0.3, 9-12 days) and donor mares in the PG group (8.7±0.7, 4-12 days). Among donor mares, the synchrony of ovulations was higher following the P&E regimen (P<0.05); however, there was a tendency (P<0.06) for fewer ovulations than in the PG group (1.5±0.3 vs. 2.5±0.4 ovulations, respectively). Embryo recovery (0.9±0.3 vs. 1.4±0.3 embryo/recovery) and recipient pregnancy rate per transferred embryo (4/9, 44% vs. 4/15, 27%) were similar. It was concluded that the P&E regimen was more reliable for synchronization of oestrus in eFSH-treated mares but the fewer ovulations may curtail any advantage of this regimen. PMID:20392656

  1. Symptoms at the menopausal and premenopausal years: their relationship with insulin, glucose, cortisol, FSH, prolactin, obesity and attitudes towards sexuality.

    PubMed

    Huerta, R; Mena, A; Malacara, J M; de León, J D

    1995-01-01

    The symptoms of 81 premenopausal and 70 menopausal women were studied to determine the association with obesity, attitudes towards sexuality (ATS), and diverse hormone values: fasting and postprandial glucose (FG, PG) and insulin (FI, PI), cortisol, prolactin, follicle-stimulating hormone (FSH). The mean age of the women studied was 49.1 years. The frequency of symptoms was 35.4% for depression, 34.3% for nonspecific symptoms of depression (NSSD), 38.6% for empty nest syndrome (ENS), and 42.3% for anxiety. NSSD, ENS, FSH and cortisol levels all possessed higher values at late-menopausal stage. A multiple regression analysis revealed the following results; NSSD was associated to ATS (negative); sleep alterations were correlated to prolactin, FSH, PI/PG, FI/FG and waist/hip ratio; FSH was associated with both a decreased sexual interest and depression. In the study of hormone levels it was found that cortisol, insulin and FI/FG were associated with ATS; PI, cortisol, FI/FG and PI/PG were associated with body mass index (BMI) and FSH; prolactin and FI/FG were associated with age. We concluded that: (1) data indicative of insulin resistance correlated to both depression and sleep alterations; (2) overweight is related to NSSD, sleep alterations, and hormonal changes. PMID:8834092

  2. FSH regulates fat accumulation and redistribution in aging through the G?i/Ca(2+)/CREB pathway.

    PubMed

    Liu, Xin-Mei; Chan, Hsiao Chang; Ding, Guo-Lian; Cai, Jie; Song, Yang; Wang, Ting-Ting; Zhang, Dan; Chen, Hui; Yu, Mei Kuen; Wu, Yan-Ting; Qu, Fan; Liu, Ye; Lu, Yong-Chao; Adashi, Eli Y; Sheng, Jian-Zhong; Huang, He-Feng

    2015-06-01

    Increased fat mass and fat redistribution are commonly observed in aging populations worldwide. Although decreased circulating levels of sex hormones, androgens and oestrogens have been observed, the exact mechanism of fat accumulation and redistribution during aging remains obscure. In this study, the receptor of follicle-stimulating hormone (FSH), a gonadotropin that increases sharply and persistently with aging in both males and females, is functionally expressed in human and mouse fat tissues and adipocytes. Follicle-stimulating hormone was found to promote lipid biosynthesis and lipid droplet formation; FSH could also alter the secretion of leptin and adiponectin, but not hyperplasia, in vitro and in vivo. The effects of FSH are mediated by FSH receptors coupled to the G?i protein; as a result, Ca(2+) influx is stimulated, cAMP-response-element-binding protein is phosphorylated, and an array of genes involved in lipid biosynthesis is activated. The present findings depict the potential of FSH receptor-mediated lipodystrophy of adipose tissues in aging. Our results also reveal the mechanism of fat accumulation and redistribution during aging of males and females. PMID:25754247

  3. FSH regulates fat accumulation and redistribution in aging through the G?i/Ca2+/CREB pathway

    PubMed Central

    Liu, Xin-Mei; Chan, Hsiao Chang; Ding, Guo-Lian; Cai, Jie; Song, Yang; Wang, Ting-Ting; Zhang, Dan; Chen, Hui; Yu, Mei Kuen; Wu, Yan-Ting; Qu, Fan; Liu, Ye; Lu, Yong-Chao; Adashi, Eli Y; Sheng, Jian-Zhong; Huang, He-Feng

    2015-01-01

    Increased fat mass and fat redistribution are commonly observed in aging populations worldwide. Although decreased circulating levels of sex hormones, androgens and oestrogens have been observed, the exact mechanism of fat accumulation and redistribution during aging remains obscure. In this study, the receptor of follicle-stimulating hormone (FSH), a gonadotropin that increases sharply and persistently with aging in both males and females, is functionally expressed in human and mouse fat tissues and adipocytes. Follicle-stimulating hormone was found to promote lipid biosynthesis and lipid droplet formation; FSH could also alter the secretion of leptin and adiponectin, but not hyperplasia, in vitro and in vivo. The effects of FSH are mediated by FSH receptors coupled to the G?i protein; as a result, Ca2+ influx is stimulated, cAMP-response-element-binding protein is phosphorylated, and an array of genes involved in lipid biosynthesis is activated. The present findings depict the potential of FSH receptor-mediated lipodystrophy of adipose tissues in aging. Our results also reveal the mechanism of fat accumulation and redistribution during aging of males and females. PMID:25754247

  4. Recombinant LH administration in subsequent cycle after "unexpected" poor response to recombinant FSH monotherapy.

    PubMed

    Papaleo, Enrico; Vanni, Valeria Stella; Viganò, Paola; La Marca, Antonio; Pagliardini, Luca; Vitrano, Rossella; Zanirato, Mara; Marsiglio, Elena; Privitera, Laura; Candiani, Massimo

    2014-11-01

    Abstract Poor ovarian response (POR) is most frequently linked to the condition known as diminished ovarian reserve, but it can also occur in the absence of pathological ovarian reserve tests ("unexpected" POR). Because possible explanations include theca cells function deficiency, our aim was to evaluate the effect of r-LH administration in "unexpected" poor responders. A retrospective, single-centre, cohort study was conducted on 65 patients with AMH >0.5?ng/ml and/or AFC >5 with POR in their first cycle. Patients underwent a second IVF cycle with same protocol (long- or antagonist) and same starting dose of r-FSH used in the first cycle, plus daily addiction of 150?IU of r-LH from day 1. Compared to the first cycle, r-LH addition in the second cycle determined an increase in number of oocytes retrieved (p?FSH starting dose, possibly due to an underlying occult theca cells deficiency. PMID:24968088

  5. FSH different regimes affect the developmental capacity and cryotolerance of embryos derived from oocytes collected by ovum pick-up in donor sheep

    Microsoft Academic Search

    F. Berlinguer; G. Leoni; L. Bogliolo; P. P. Pintus; I. Rosati; S. Ledda; S. Naitana

    2004-01-01

    The objective of the present study was to compare the developmental capacity of sheep oocytes obtained by OPU after two different ovarian stimulations, and cryotolerance to vitrification procedures of in vitro derived embryos after in vitro maturation, fertilisation and culture of these oocytes. Sheep were divided into three groups: (A) no treatment (control); (B) constant doses of FSH (FSH-c); (C)

  6. A synthetic peptide encompassing two discontinuous regions of hFSH-beta subunit mimics the receptor binding surface of the hormone.

    PubMed

    Santa-Coloma, T A; Crabb, J W; Reichert, L E

    1991-07-01

    Synthetic peptides corresponding to discontinuous segments of the hFSH-beta subunit, amino acids 33-53 and 81-95, have been shown to interact with the follicle-stimulating hormone (FSH) receptor. In this study, we demonstrate that hFSH-beta-(33-53)-(81-95)-peptide amide, a synthetic peptide encompassing these binding regions, possesses higher affinity for the FSH receptor than either synthetic hFSH-beta-(33-53) or hFSH-beta-(81-95). This increased affinity suggests that each binding component is effectively interacting with the receptor, providing evidence that these two separate receptor binding regions of hFSH-beta form a continuous binding surface on the native molecule. These results also suggest that binding surfaces of very complex proteins, such as the heterodimeric glycoprotein hormone FSH, may be mimicked by a linear arrangement of its binding domains. A model based on energetics of the peptide-receptor interaction is also described. The results indicate that the affinity (Ka) of a peptide containing different binding domains can be approximated utilizing the product of the affinity constant of each binding domain (Ka = k1.k2...kn). PMID:1778304

  7. Prsente par Maxime DARNON

    E-print Network

    Paris-Sud XI, Université de

    OCH poreux nécessite de revoir les différents procédés par plasmas (gravure, traitements post-gravure) et les des matériaux poreux et hybrides par les plasmas post-gravure est également étudiée. Avec un plasma fluorocarboné, le matériau hybride présente des mécanismes de gravure similaires à ceux d'un SiOCH dense. Le Ti

  8. The role of testicular fluid on blood plasma levels of FSH and LH in the ram (1)

    E-print Network

    Paris-Sud XI, Université de

    castration. Blood plasma was immediately separated by centrifugation at 1 500 g and stored at ― 20 °CThe role of testicular fluid on blood plasma levels of FSH and LH in the ram (1) M. R. BLANC J. L was still functional. Two daily blood samples were taken from 7 days before cannulation to 7 days after

  9. FSH response in prepubertal male and female rats to inhibin-like activity as present in follicular fluid.

    E-print Network

    Paris-Sud XI, Université de

    FSH response in prepubertal male and female rats to inhibin-like activity as present in follicular (Welschen et al., 1977 ; Marder, Channing and Schwartz, 1977) rats. Experiments in adult female rats show was reached by Nandini, Lipner and Moudgal (1976) who injected immature male rats with inhibin of testicular

  10. Effect of recombinant human FSH and LH on in vitro maturation of sheep oocytes; embryo development and viability

    Microsoft Academic Search

    C. Accardo; M. Dattena; S. Pilichi; L. Mara; B. Chessa; P. Cappai

    2004-01-01

    The objective of this work was to study the effect of a preparation of human recombinant gonadotrophins (r-FSH and r-LH) on the in vitro maturation (IVM) and development of sheep oocytes. In addition, the viability of fresh and vitrified blastocysts obtained after transfer was tested. Oocytes collected from slaughtered animals were divided into five different maturation groups. All groups were

  11. Molecular cloning and expression analysis of female sterile homeotic gene (fsh) in the oriental river prawn Macrobrachium nipponense.

    PubMed

    Zhang, W Y; Jiang, S F; Xiong, Y W; Fu, H T; Qiao, H; Sun, S M; Gong, Y S; Jin, S B

    2015-01-01

    The gene female sterile homeotic (fsh) plays crucial roles in molecular function, including protein kinase activity and DNA binding, which are involved in biological processes such as terminal region determination and negative regulation of DNA-dependent transcription. Although fsh has been found in Drosophila melanogaster, little is known regarding its expression in crustaceans. In this study, a fsh gene homologue, designated as Mnfsh, was cloned and characterized from the testis of the oriental river prawn, Macrobrachium nipponense, by using EST analysis and the RACE approach for the first time. The full-length cDNA of Mnfsh was 2029 bp, consisting of a 5' UTR of 361 bp, a 3' UTR of 216 bp, and an ORF of 1452 bp encoding 484 amino acids. qRT-PCR analysis showed that the Mnfsh gene was expressed in the testis, ovary, muscle, heart, eyestalk, and abdominal ganglion, with the highest level of expression in the ovary and the lowest in the heart. qRT-PCR analyses showed that the expression levels of Mnfsh mRNA both significantly increased in the zoea stage, the VII larvae, and 1st day post-larvae after metamorphosis. In conclusion, the results of the present study indicate that Mnfsh is an arthropod fsh homologue and probably also plays important roles in embryogenesis, organogenesis, and morphological differentiation of M. nipponense. PMID:25966204

  12. Radiotherapy for Rectal Cancer Is Associated With Reduced Serum Testosterone and Increased FSH and LH

    SciTech Connect

    Bruheim, Kjersti [Cancer Center, Ullevaal University Hospital, Oslo (Norway)], E-mail: Kjersti.Bruheim@medisin.uio.no; Svartberg, Johan [Institute of Clinical Medicine, University of Tromso, Tromso (Norway); Department of Medicine, University Hospital of North Norway, Tromso (Norway); Carlsen, Erik [Department of Gastrointestinal Surgery, Ullevaal University Hospital, Oslo (Norway); Dueland, Svein [Department of Oncology, Norwegian Radium Hospital, Oslo (Norway); Haug, Egil [Hormone Laboratory, Aker University Hospital, Oslo (Norway); Skovlund, Eva [School of Pharmacy, University of Oslo, Oslo (Norway); Tveit, Kjell Magne; Guren, Marianne G. [Cancer Center, Ullevaal University Hospital, Oslo (Norway)

    2008-03-01

    Purpose: It is known that scattered radiation to the testes during pelvic radiotherapy can affect fertility, but there is little knowledge on its effects on male sex hormones. The aim of this study was to determine whether radiotherapy for rectal cancer affects testosterone production. Methods and Materials: All male patients who had received adjuvant radiotherapy for rectal cancer from 1993 to 2003 were identified from the Norwegian Rectal Cancer Registry. Patients treated with surgery alone were randomly selected from the same registry as control subjects. Serum levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), testosterone, and sex hormone binding globulin (SHBG) were analyzed, and free testosterone was calculated (N = 290). Information about the radiotherapy treatment was collected from the patient hospital charts. Results: Serum FSH was 3 times higher in the radiotherapy group than in the control group (median, 18.8 vs. 6.3 IU/L, p <0.001), and serum LH was 1.7 times higher (median, 7.5 vs. 4.5 IU/l, p <0.001). In the radiotherapy group, 27% of patients had testosterone levels below the reference range (8-35 nmol/L), compared with 10% of the nonirradiated patients (p <0.001). Irradiated patients had lower serum testosterone (mean, 11.1 vs. 13.4 nmol/L, p <0.001) and lower calculated free testosterone (mean, 214 vs. 235 pmol/L, p <0.05) than control subjects. Total testosterone, calculated free testosterone, and gonadotropins were related to the distance from the bony pelvic structures to the caudal field edge. Conclusions: Increased serum levels of gonadotropins and subnormal serum levels of testosterone indicate that curative radiotherapy for rectal cancer can result in permanent testicular dysfunction.

  13. [Hormone profile and follicle development in ovarian stimulation treatment for in vitro fertilization. 1. FSH and LH profiles].

    PubMed

    Sudik, R; Fliess, F R; Kunkel, S

    1987-01-01

    144 patients were treated by 5 different stimulation protocols in the IVF-program of the University Women's Hospital Rostock (Clomiphene/HCG, Clomiphene/Anthrogon/HCG, Pergonal/HCG, Anthrogon/HCG, Folistiman/HCG). In 172 cycles FSH and LH were measured in serum during the follicular phase by RIA. The results were compared between the treatment groups and a group of normal women by Student's t-test or Mann-Whitney's nonparametric test. Moreover, in each group the number of follicles visible by laparoscopy and number of punctured follicles were estimated. The maturation of collected oocytes were judged by a maturation index. Modus of action and FSH/LH-ratio of used drugs have been shown to influence serum levels of FSH. Every stimulation protocol was characterized by typical profiles of hormones. Especially, immunoreactive levels of FSH were related to development of follicles. Different biological action of various stimulation regimes was demonstrated by a shortage of follicular phase from 13,4 days in the Clomiphene group to 11,0 days in the Folistiman group, an increase of laparoscopically visible follicles from 4,0 to 13,2 and a rise of collected oocytes from 1,6 to 7,3 per laparoscopy. Quality of oocytes has been shown to be related significantly to the modus of stimulation. Anthrogon-protocol was the most effective stimulation regime according to profile of gonadotropins, the number of follicles and the structure of harvested population of oocytes. However, even in this group co-dominant development of follicle was not achieved. Application of pure FSH and an earlier start of stimulation treatment are possibilities for improvements of results in IVF by stimulation. PMID:3126616

  14. Metformin Inhibits Follicle-Stimulating Hormone (FSH) Action in Human Granulosa Cells: Relevance to Polycystic Ovary Syndrome

    PubMed Central

    Elia, Androulla; Jawad, Zara; Pellatt, Laura; Mason, Helen D.

    2013-01-01

    Background: Women with anovulatory polycystic ovary syndrome (PCOS) are generally insulin-resistant and as a consequence are often treated with the biguanide metformin. Results with metformin have, however, been variable with some studies demonstrating induction of regular cycles and an increase in ovulation, whereas others do not. Hence more understanding is needed regarding the mechanism of metformin's actions in ovarian granulosa cells especially in light of previous demonstrations of direct actions. Objective: The aim of this study was to investigate metformin's interaction with the FSH/cAMP/protein kinase A pathway, which is the primary signaling pathway controlling CYP19A1 (aromatase) expression in the ovary. Methods: The effect of metformin on FSH and forskolin-stimulated aromatase expression in human granulosa cells was measured by quantitative real-time PCR. Activity was assessed after transfection with a promoter II-luciferase construct, and by an RIA measuring conversion of androgen to estrogens. The effect on FSH receptor (FSHR) mRNA was assessed by quantitative PCR. Levels of phosphorylated cAMP response element binding protein (CREB) and CREB-regulated transcription coactivator 2 (CRTC2) were measured by Western blotting and cAMP by a bioluminescent assay. Results: Metformin markedly reduced FSH but not forskolin-stimulated aromatase expression and activity. This effect was exerted by inhibition of basal and ligand-induced up-regulation of FSHR expression. Metformin also reduced FSH-induced phosphorylation of CREB and hence CRE activity, which could potentially disrupt the CREB–CREB-binding protein–CRTC2 coactivator complex that binds to CRE in promoter II of the aromatase gene. This is mediated in an AMP-activated protein kinase-independent manner, and does not involve alteration of cAMP levels. Conclusion: These finding have implications for the use of metformin in the treatment of anovulation in women with PCOS. PMID:23846817

  15. Cost-effectiveness analysis on the use of rFSH + rLH for the treatment of anovulation in hypogonadotropic hypogonadal women

    PubMed Central

    Papaleo, Enrico; Alviggi, Carlo; Colombo, Giorgio Lorenzo; Pisanelli, Claudio; Ripellino, Claudio; Longobardi, Salvatore; Canonico, Pier Luigi

    2014-01-01

    Background Hypogonadotropic hypogonadal women are characterized by ovarian functionality deficiency, caused by low concentrations of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). To recover reproduction functionality, recommended therapies for ovarian induction involve injections of FSH and LH medications. Objective Since important differences exist between recombinant and urinary gonadotropin therapies in terms of efficacy and cost, the objective of this study was to develop a cost-effectiveness model to compare recombinant FSH (rFSH) + recombinant LH (rLH) and highly purified human menopausal gonadotropin (HP-HMG). Methods A Markov model was developed, considering three cycles of therapy; probability of pregnancy and miscarriage were considered, and the efficacy was evaluated in terms of pregnancy occurrence. The perspective of the model was that of the Italian Health Service, so only direct cost (drugs, specialist visits, patient examinations, and hospitalizations) were included. Results rFSH + rLH is associated with a higher total cost (€3,453.50) and higher efficacy (0.87) compared with HP-HMG (€2,719.70 and 0.50). rFSH + rLH generated an incremental cost effectiveness ratio equal to €2,007.30 compared to HP-HMG; the average cost per pregnancy is estimated to be €3,990.00 for recombinant strategy and €5,439.80 for urinary strategy. Results of probabilistic sensitivity analysis were consistent with the abovementioned findings. Conclusion Despite the higher acquisition cost in comparison to HP-HMG, rFSH + rLH resulted in a higher pregnancy rate, which makes it the recommended choice when considering cost-effectiveness of LH in supporting FSH-induced follicular gonadotropins in hypogonadotropic hypogonadal women. PMID:25028553

  16. Role of Seminiferous Tubular Development in Determining the FSH versus LH Responsiveness to GnRH in Early Sexual Maturation

    PubMed Central

    Pitteloud, Nelly; Thambundit, Apisadaporn; Dwyer, Andrew A.; Falardeau, John L.; Plummer, Lacey; Caronia, Lisa M.; Hayes, Frances J.; Lee, Hang; Boepple, Paul A.; Crowley, William F.

    2009-01-01

    Background The onset of sexual maturation at puberty is a unique developmental period from a neuroendocrine perspective in that it is characterized by enhanced FSH secretion and FSH responsiveness to exogenous GnRH (vs. LH) from the gonadotrope, yet the mechanism of these dynamics remains unclear. This study aimed to elucidate this phenomenon using a human disease model of GnRH deficiency (idiopathic hypogonadotropic hypogonadism, IHH) in which GnRH input can be experimentally controlled. Methods 25 GnRH-deficient men were selected for study based upon their baseline testicular volumes (TV) and serum inhibin B (IB) levels to represent a spectrum of pubertal/testicular development. Subjects underwent: (i) a 12-hour overnight neuroendocrine evaluation for hormonal profiling and determination of endogenous LH secretion pattern, and (ii) a 7-day exposure to a physiologic regimen of exogenous pulsatile GnRH (25 ng/kg every 2 h). Daily measurements of serum testosterone (T) and IB levels were made and a 2-hour window of frequent blood sampling was monitored to measure LH and FSH following a single i.v. GnRH bolus (25 ng/kg). All subjects were screened for known loci underlying GnRH deficiency and the response to GnRH was tracked according to genotype. Results Among the entire cohort, no changes were noted in serum T or IB during the 7 days, thus keeping gonadal feedback relatively constant. However, serum LH and FSH levels increased significantly (p < 0.0001) in the entire cohort. When analyzed by degree of pubertal/testicular development, men with no evidence of prior spontaneous pubertal development (TV ?3 ml, Group I) showed sharp increases in serum FSH compared to men with some prior evidence of partial puberty (TV >3 ml, Group II, p < 0.0001). Group I exhibited a decreased LH response to GnRH on day 2 compared to day 1 (p < 0.01), which did not recover until day 5 (1–4 vs. 5–7 days, p < 0.0001). Group II displayed robust and equivalent LH responses to GnRH throughout the 7-day study. Genetic studies identified 8 mutations in 4 different loci (DAX1, KAL1, GNRHR, and FGFR1) in this cohort. Conclusions GnRH-deficient men undergoing GnRH-induced sexual maturation display an inverse relationship between FSH responsiveness to GnRH and baseline testicular size and IB levels. This observation implies that increasing seminiferous tubule maturity represents the major constraint on FSH responsiveness to GnRH in early puberty. In contrast, LH responsiveness to GnRH correlates directly with duration of GnRH exposure. Attenuated pituitary gonadotropin responses were noted in subjects harboring DAX1 mutations, consistent with known pituitary defects. PMID:19829004

  17. Par Mohcine HASSAR Thse prsente

    E-print Network

    Paris-Sud XI, Université de

    Par Mohcine HASSAR Thèse présentée pour l'obtention du grade de Docteur de l'UTC Influence des nano;Université de Technologie de Compiègne THESE Présentée en vue d'obtenir le grade de DOCTEUR En Spécialité : Mécanique Avancée Par Mohcine HASSAR DOCTORAT DELIVRE PAR L'UNIVERSITE DE TECHNOLOGIE DE COMPIEGNE Titre de

  18. Circulating concentrations of ovarian steroids and follicle-stimulating hormone (FSH) in ewes with 3 or 4 waves of antral follicle emergence per estrous cycle.

    PubMed

    Baby, Tanya E; Bartlewski, Pawel M

    2011-03-01

    The mechanism governing the number of follicle-stimulating hormone (FSH) peaks and emerging follicular waves in ruminants remains unknown. The main purpose of the present study was to examine the relationships between progesterone (P(4)) levels, circulating concentrations of FSH and antral follicular development throughout the interovulatory interval in sheep. We retrospectively analyzed and compared daily serum concentrations of (P4), FSH and estradiol (E2) obtained in cyclic (November-December) Western White Face ewes (Columbia×Rambouillet) that had 3 (n=10) or 4 (n=19) follicular waves per estrous cycle. Follicular growth was monitored in all animals by daily transrectal ultrasonography. Mean P(4) concentrations were greater (p<0.05) in sheep with 4 waves per cycle compared to their counterparts with 3 waves of follicular growth. The ewes with 3 waves exceeded (p<0.05) animals with 4 follicular waves in mean serum FSH concentrations on days 0-2, 6, 7, 9-11, 14 and 15 post-ovulation. Animals with 4 follicular waves exceeded (p<0.05) the ewes with 3 waves in mean serum E(2)> concentrations on days - 1, 2 and 10 of the estrous cycle studied (day 0=ovulation). The present results are supportive of the notion that luteal P(4) is an important endocrine signal, which controls the periodicity of FSH peaks and the number of emerging follicular waves in cyclic ewes. PMID:21455278

  19. Production of recombinant orange-spotted grouper (Epinephelus coioides) follicle-stimulating hormone (FSH) in single-chain form and dimer form by Pichia pastoris and their biological activities.

    PubMed

    Chen, Jun; Zhang, Yanhong; Tang, Zhiguo; Mao, Jiewei; Kuang, Zhonglei; Qin, Chaobin; Li, Wensheng

    2012-09-01

    FSH is a key regulator of steroidogenesis and gonadal growth in teleosts. However, function of FSH is elusive in grouper due to the lack of purified and native FSH. In the present study, we reported production of bioactive orange-spotted grouper (Epinephelus coioides) FSH in dimer form and single-chain form by Pichia pastoris. Dimer form of recombinant grouper FSH (rgFSHba) was accomplished by co-expressing mature FSHb-subunit and a-subunit genes. Fusion of mature FSHb-subunit and a-subunit genes together linking with a polypeptide (4×(Gly-Ser)-Gly-Thr) gene generated single-chain form of recombinant grouper FSH (rgFSHb-a). Recombinant grouper common ?-subunit (rgCga) and FSHb-subunit (rgFSHb) were also separately produced. Recombinant proteins were verified by Western blot and mass spectrometry assays, and characterized by deglycosylation analysis. Deglycosylation assay suggested that glycosylation of recombinant FSH mainly occurred on common a-subunit. Bioactivities of recombinant proteins were initially evaluated by activating grouper FSH receptor, and further demonstrated by incubating ovarian fragments of adult grouper and intraperitoneal injection in juvenile female grouper. Two forms of recombinant FSH presented similar biological activities of activating FSH receptor and stimulating in vitro testosterone (T) and estradiol-17? (E2) secretion, though the dimer form functioned slightly weaker than the single-chain form. However, injections of rgFSHb-a or rgFSHba could significantly increase serum T and E2 levels, induce early ovarian development, reduce hypothalamic gnrh1 mRNA level, and increase hypothalamic cyp19a1b mRNA level. Data in this study suggested that recombinant gonadotropin could be produced in dimer form or single-chain form by P. pastoris, and FSH could regulate steroidogenesis and early ovarian development in juvenile grouper. PMID:22684083

  20. Effects of growth differentiation factor-9 and FSH on in vitro development, viability and mRNA expression in bovine preantral follicles.

    PubMed

    Vasconcelos, G L; Saraiva, M V A; Costa, J J N; Passos, M J; Silva, A W B; Rossi, R O D S; Portela, A M L R; Duarte, A B G; Magalhães-Padilha, D M; Campelo, C C; Figueiredo, J R; van den Hurk, R; Silva, J R V

    2013-01-01

    The present study investigated the role of growth differentiation factor (GDF)-9 and FSH, alone or in combination, on the growth, viability and mRNA expression of FSH receptor, proliferating cell nuclear antigen (PCNA) and proteoglycan-related factors (i.e., hyaluronan synthase (HAS) 1, HAS2, versican, perlecan) in bovine secondary follicles before and after in vitro culture. After 12 days culture, sequential FSH (100 ng mL?¹) from Days 0 to 6 and 500 ng mL?¹ from Days 7 to 12) increased follicular diameter and resulted in increased antrum formation (P<0.05). Alone, 200 ng mL?¹ GDF-9 significantly reduced HAS1 mRNA levels, but increased versican and perlecan mRNA levels in whole follicles, which included the oocyte, theca and granulosa cells. Together, FSH and GDF-9 increased HAS2 and versican (VCAN) mRNA levels, but decreased PCNA mRNA expression, compared with levels in follicles cultured in ?-minimum essential medium supplemented with 3.0 mg mL?¹ bovine serum albumin, 10 µg mL?¹ insulin, 5.5 µg mL?¹ transferrin, 5 ng mL?¹ selenium, 2 mM glutamine, 2mM hypoxanthine and 50 ?g mL?¹ ascorbic acid (?-MEM?). Comparisons of uncultured (0.2 mm) and ?-MEM? cultured follicles revealed that HAS1 mRNA expression was higher, whereas VCAN expression was lower, in cultured follicles (P<0.05). Expression of HAS1, VCAN and perlecan (HSPG2) was higher in cultured than in vivo-grown (0.3 mm) follicles. In conclusion, FSH and/or GDF-9 promote follicular growth and antrum formation. Moreover, GDF-9 stimulates expression of versican and perlecan and interacts positively with FSH to increase HAS2 expression. PMID:23241220

  1. A common polymorphic allele of the LH beta-subunit gene is associated with higher exogenous FSH consumption during controlled ovarian stimulation for assisted reproductive technology

    PubMed Central

    2013-01-01

    Background V-betaLH is a common genetic variant of LH caused by two polymorphic base changes in the beta subunit gene, altering the amino acid sequence (Trp8Arg and Ile15Thr). In a previous-preliminary trial performed in women undergoing IVF, it was demonstrated that carriers of v-betaLH show sub-optimal ovarian response to a standard long GnRH-agonist down -regulation protocol when stimulated with pure recombinant FSH (r-hFSH). The aim of this study was to confirm the hypothesis that women with v-betaLH display hypo-sensitivity to exogenous FSH in a larger IVF population and to explore the frequency of this variant in a Danish female population. Methods In the present study, the effect of v-betaLH was retrospectively investigated in a larger series of women undergoing controlled ovarian stimulation (COS) and, for the first time, in a Danish IVF population. A total of 220 normogonadotrophic women following a long GnRH-agonist down-regulation protocol received an individualized dose of r-hFSH (100 IU and 375 IU s.c. daily) according to antral follicle count, baseline FSH, body mass index and age. The LH genotype was assessed in all patients by immunofluorometric assay. Results V-betaLH was present in 11% of patients, whereas the allelic frequency was 12%. The study population was divided into two groups according to their LH genotype. Group A consisted of 196 wt/wt women. Group B included 24 individuals with v-betaLH (21 heterozygous and 3 homozygous). No statistically significant differences in the mean number of oocytes retrieved, fertilization rate and pregnancy rate per cycle were observed between groups. However, Group B received a significantly higher cumulative-dose of r-hFSH than Group A (2435.86 +/? 932.8 IU versus 1959.8 +/? 736.45 p?=?0.048). When one-way ANOVA in a within design was applied, the LH genotype had a statistically significant effect (p?FSH, showing a progressive increase from wt/wt (1959.8 +/? 736.45 IU) to v-betaLH hetero- (2267.5 +/? 824.3) and homozygotic women (3558.3 +/? 970.9). Conclusions These results confirm that carriers exhibit hypo-sensitivity to exogenous FSH during COS, documenting that the frequency of v-betaLH in Denmark is similar to a number of European countries. PMID:23725475

  2. Brulures par Diluant

    PubMed Central

    Benbrahim, A.; Jerrah, H.; Diouri, M.; Bahechar, N.; Boukind, E.H.

    2009-01-01

    Summary La flamme de diluant est une cause non rare de brûlure dans le contexte marocain. Nous avons jugé intéressant de faire une étude épidémiologique sur la brûlure par flamme de diluant (BFD) au centre national des brûlés (CNB) du CHU Ibn-Rochd de Casablanca. Ce travail a été réalisé sur une période de 10 mois (septembre 2007/juin 2008). Le but du travail est de montrer les caractéristiques de ce type de brûlures pour les prévenir et ce par l'information sur le diluant, produit causant ces brûlures, et ses différents dangers, la brûlure notamment. Durant cette période, nous avons colligé 17 cas de BFD sur un total de 356 patients admis au CNB pour brûlures aiguës toute étiologie confondue. La moyenne d'age des patients concernés est de 32 ans. Ils sont presque tous de sexe masculin (16 hommes/1 femme) et ont des antécédents de toxicomanie et/ou de délinquance. Tous nos patients sont de bas niveau socio-économique et habitent dans des bidonvilles pour la plupart. La brûlure est souvent secondaire à une agression dans la rue (92% des cas). Concernant les caractéristiques de la brûlure, la surface cutanée brûlée moyenne est de 23%; elle est souvent profonde et siège surtout au niveau des membres supérieurs et du tronc. PMID:21991179

  3. THSE DE DOCTORAT Prsente par

    E-print Network

    Paris-Sud XI, Université de

    (Maître de Conférences HDR, Université de Toulon) Directeur de thèse Asma SAKKA HLAILI (Professeur desAvantAvantAvant----ProposProposProposPropos Cette thèse a été co-dirigée par une partie tunisienne et par une partie française. En Tunisie, Mme Asma impactées : Faouzi HERZI, Asma SAKKA HLAILI, Christophe LE POUPON, Hassine HADJ MABROUK, Stéphane MOUNIER

  4. Use of fluorogestone acetate after breeding to reduce the effect of premature luteal regression in dairy goats when superovulation is induced with FSH

    Microsoft Academic Search

    M. J. Cervantes; M. L. Juárez; V. O. Mejía; V. J. M. Berruecos; A. H. Vera; J. Valencia

    2007-01-01

    The aim of the present study was to determine the effect of fluorogestone acetate (FGA) administered after mating, on embryo production in the dairy goat subjected to conventional superovulatory and embryo recovery protocols. Adult does, most of them of the French Alpine breed, were randomly assigned after a FSH-superovulatory estrus and fertile matings to a control group (n=20) or to

  5. Comparison of recombinant human follicle stimulating hormone (rhFSH), human chorionic gonadotropin (HCG) and human menopausal gonadotropin (HMG) on semen parameters after varicocelectomy: a randomized clinical trial

    PubMed Central

    Amirzargar, Mohammad Ali; Yavangi, Mahnaz; Basiri, Abbass; Hosseini Moghaddam, Sayyed Mahdi; Babbolhavaeji, Hooshang; Amirzargar, Nasibeh; Amirzargar, Hossein; Moadabshoar, Leila

    2012-01-01

    Background: The most frequent physical finding in infertile men is varicocele, in which one of the mechanisms that can affect seminal parameters is oxidative stress. Objective: Our study aimed, for the first time, to compare the efficacy of recombinant human follicle-stimulating hormone (rhFSH), human chorionic gonadotropin (HCG) and human menopausal gonadotropin (HMG) on sperm parameters and fertility after varicocelectomy. Materials and Methods: 113 infertile men with varicocele were divided into four groups. Group A received HCG 5000 IU weekly, group B received HMG 75 IU three times a week, group C received rhFSH 75 IU three times a week and group D received no medical treatment after varicocelectomy. Results: After three months, in group A sperm morphology improved (p=0.007), causing a 32% pregnancy rate. In group B, sperm motility (p=0.023) and morphology (p=0.014) improved, causing a 57% pregnancy rate. In group C, all of the investigated semen parameters increased (p<0.05), causing a 62.5% pregnancy rate. Only rhFSH improved sperm concentrations to >20×106 mL (p=0.027). In group D, sperm morphology increased (p=0.038), but other parameters remained unchanged and no pregnancies occurred. Conclusion: It can be concluded that drugs can reduce induction time for spermatogenesis and fertility in comparison with varicocelectomy alone. For these purposes, rhFSH is more effective than other drugs. PMID:25246910

  6. Correlations of Insulin Resistance and Serum Testosterone Levels with LH:FSH Ratio and Oxidative Stress in Women with Functional Ovarian Hyperandrogenism.

    PubMed

    Suresh, S; Vijayakumar, T

    2015-07-01

    Functional ovarian hyperandrogenism (FOH) is a form of polycystic ovary syndrome (PCOS) characterized by elevated circulating levels of androgens derived from the ovary. Insulin resistance (IR) is the most common etiological factor in women with FOH. IR causes the generation of increased oxidative stress (OS) and diminished antioxidant status. OS is directly correlated with both IR and testosterone levels, which consequently contribute to endocrine and biochemical alterations in FOH women. In the current study, elevations in total testosterone, free testosterone and luteinizing hormone (LH) levels accompanied by a decrease in follicle stimulating hormone (FSH) level leading to higher LH:FSH ratio were the prominent endocrine changes observed in women with FOH. A significant increase in fasting blood levels of glucose and insulin, as well as an elevated IR were also seen in FOH women, as compared to their age matched controls. Women with FOH have higher pro-oxidant and lower anti-oxidant levels in blood than their age matched controls. In FOH women, elevations in LH:FSH ratio and OS are correlated more with hyperandrogenemia than with IR. Of the androgens, free rather than total testosterone has better positive correlations with elevated LH:FSH ratio and OS, and hence, the former is a better predictive marker for the development of biochemical PCOS in women with FOH. PMID:26089623

  7. Associations between tamoxifen, estrogens, and FSH serum levels during steady state tamoxifen treatment of postmenopausal women with breast cancer

    PubMed Central

    2010-01-01

    Background The cytochrome P450 (CYP) enzymes 2C19, 2D6, and 3A5 are responsible for converting the selective estrogen receptor modulator (SERM), tamoxifen to its active metabolites 4-hydroxy-tamoxifen (4OHtam) and 4-hydroxy-N-demethyltamoxifen (4OHNDtam, endoxifen). Inter-individual variations of the activity of these enzymes due to polymorphisms may be predictors of outcome of breast cancer patients during tamoxifen treatment. Since tamoxifen and estrogens are both partly metabolized by these enzymes we hypothesize that a correlation between serum tamoxifen and estrogen levels exists, which in turn may interact with tamoxifen on treatment outcome. Here we examined relationships between the serum levels of tamoxifen, estrogens, follicle-stimulating hormone (FSH), and also determined the genotypes of CYP2C19, 2D6, 3A5, and SULT1A1 in 90 postmenopausal breast cancer patients. Methods Tamoxifen and its metabolites were measured by liquid chromatography-tandem mass spectrometry. Estrogen and FSH levels were determined using a sensitive radio- and chemiluminescent immunoassay, respectively. Results We observed significant correlations between the serum concentrations of tamoxifen, N-dedimethyltamoxifen, and tamoxifen-N-oxide and estrogens (p < 0.05). The genotype predicted CYP2C19 activity influenced the levels of both tamoxifen metabolites and E1. Conclusions We have shown an association between tamoxifen and its metabolites and estrogen serum levels. An impact of CYP2C19 predicted activity on tamoxifen, as well as estrogen kinetics may partly explain the observed association between tamoxifen and its metabolites and estrogen serum levels. Since the role of estrogen levels during tamoxifen therapy is still a matter of debate further prospective studies to examine the effect of tamoxifen and estrogen kinetics on treatment outcome are warranted. PMID:20565970

  8. Female Mice Expressing Constitutively Active Mutants of FSH Receptor Present with a Phenotype of Premature Follicle Depletion and Estrogen Excess

    PubMed Central

    Peltoketo, Hellevi; Strauss, Leena; Karjalainen, Riikka; Zhang, Meilin; Stamp, Gordon W.; Segaloff, Deborah L.; Poutanen, Matti; Huhtaniemi, Ilpo T.

    2010-01-01

    Strong gain-of-function mutations have not been identified in humans in the FSH receptor (FSHR), whereas such mutations are common among many other G protein-coupled receptors. In order to predict consequences of such mutations on humans, we first identified constitutively activated mutants of the mouse (m) Fshr and then expressed them under the human anti-Müllerian hormone promoter in transgenic mice or created knock-in mutation into the mouse genome. We show here that mutations of Asp580 in the mFSHR significantly increase the basal receptor activity. D580H and D580Y mutations of mFSHR bind FSH, but the activity of the former is neither ligand-dependent nor promiscuous towards LH/human choriogonadotropin stimulation. Transgenic expression of mFshrD580H in granulosa cells leads to abnormal ovarian structure and function in the form of hemorrhagic cysts, accelerated loss of small follicles, augmented granulosa cell proliferation, increased estradiol biosynthesis, and occasional luteinized unruptured follicles or teratomas. The most affected mFshrD580H females are infertile with disturbed estrous cycle and decreased gonadotropin and increased prolactin levels. Increased estradiol and prolactin apparently underlie the enhanced development of the mammary glands, adenomatous pituitary growth, and lipofuscin accumulation in the adrenal gland. The influence of the mFSHRD580Y mutation is milder, mainly causing hemorrhagic cysts in transgenic mFSHRD580Y and mFSHRD580Y -knock-in mice. The results demonstrate that gain-of-function mutations of the FSHR in mice bring about distinct and clear changes in ovarian function, informative in the search of similar mutations in humans. PMID:20172968

  9. On the mechanism of action of lead in the testis: in vitro suppression of FSH receptors, cyclic AMP and steroidogenesis. [Rats

    SciTech Connect

    Wiebe, J.P.; Salhanick, A.I.; Myers, K.I.

    1983-04-25

    The purpose of the present study was to determine by in vitro methods, if Pb acts by interfering directly with hormone binding, cyclic AMP production and steroidogenic enzyme activity. Sertoli cells were isolated from testes of prepubertal rats and cultured in the presence of 2.64 x 10/sup -4/ M of either NaAc (control) or PbAc for 1, 4, 24, 48, 96 or 144 hr. There was no reduction in FSH binding and in FSH-induced cyclic AMP after a 1-4 hr exposure to Pb. After a 24-hr exposure to Pb, the cells exhibited a 10-20% decrease in FSH binding and cyclic AMP production and after 96 hr there was a 75% decrease in these 2 parameters. The inhibition was greater in cells from 16 day old than from 20 day old rats, so that in the former, after a 144 hr exposure the FSH-induced cyclic AMP of the Pb exposed cells was only 3% of the amount produced by the NaAc exposed cells (i.e. a 97% inhibition). After in vitro exposure to Pb for 48 hr, the steroidogenic activity (progesterone conversion to steroid metabolites) of Sertoli cells was significantly reduced and their steroidogenesis was no longer stimulated by FSH. A crude testicular enzyme preparation containing 3..beta..-hydroxysteroid dehydrogenase (3..beta..-HSD) exhibited approximately 25% reduction in activity if the assay buffer contained PbCl/sub 2/ instead of the equivalent in NaCl. Prolonged in vivo exposure to Pb resulted in approximately 50% reduction in 3..beta..-HSD activity. This is the first indication that in the testis Pb may act directly (immediate effect) by suppressing enzyme activities, and indirectly (long term effect) by reducing gonadotropin-receptor binding and the resultant cyclic AMP production.

  10. Par Pond vegetation status 1996

    SciTech Connect

    Mackey, H.E. Jr.; Riley, R.S.

    1996-12-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the newly emergent, shoreline aquatic plant communities of Par Pond began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level. These surveys continued in July, September, and late October, 1995, and into the early spring and late summer of 1996. Communities similar to the pre-drawdown, Par Pond aquatic plant communities continue to become re-established. Emergent beds of maidencane, lotus, waterlily, watershield, and Pontederia are extensive and well developed. Measures of percent cover, width of beds, and estimates of area of coverage with satellite data indicate regrowth within two years of from 40 to 60% of levels prior to the draw down. Cattail occurrence continued to increase during the summer of 1996, especially in the former warm arm of Par Pond, but large beds common to Par Pond prior to the draw down still have not formed. Lotus has invaded and occupies many of the areas formerly dominated by cattail beds. To track the continued development of macrophytes in Par Pond, future surveys through the summer and early fall of 1997, along with the evaluation of satellite data to map the extent of the macrophyte beds of Par Pond, are planned.

  11. Protease-activated receptor (PAR)-2 is required for PAR-1 signalling in pulmonary fibrosis.

    PubMed

    Lin, Cong; von der Thüsen, Jan; Daalhuisen, Joost; Ten Brink, Marieke; Crestani, Bruno; van der Poll, Tom; Borensztajn, Keren; Spek, C Arnold

    2015-06-01

    Idiopathic pulmonary fibrosis is the most devastating diffuse fibrosing lung disease of unknown aetiology. Compelling evidence suggests that both protease-activated receptor (PAR)-1 and PAR-2 participate in the development of pulmonary fibrosis. Previous studies have shown that bleomycin-induced lung fibrosis is diminished in both PAR-1 and PAR-2 deficient mice. We thus have been suggested that combined inactivation of PAR-1 and PAR-2 would be more effective in blocking pulmonary fibrosis. Human and murine fibroblasts were stimulated with PAR-1 and PAR-2 agonists in the absence or presence of specific PAR-1 or PAR-2 antagonists after which fibrotic markers like collagen and smooth muscle actin were analysed by Western blot. Pulmonary fibrosis was induced by intranasal instillation of bleomycin into wild-type and PAR-2 deficient mice with or without a specific PAR-1 antagonist (P1pal-12). Fibrosis was assessed by hydroxyproline quantification and (immuno)histochemical analysis. We show that specific PAR-1 and/or PAR-2 activating proteases induce fibroblast migration, differentiation and extracellular matrix production. Interestingly, however, combined activation of PAR-1 and PAR-2 did not show any additive effects on these pro-fibrotic responses. Strikingly, PAR-2 deficiency as well as pharmacological PAR-1 inhibition reduced bleomycin-induced pulmonary fibrosis to a similar extent. PAR-1 inhibition in PAR-2 deficient mice did not further diminish bleomycin-induced pulmonary fibrosis. Finally, we show that the PAR-1-dependent pro-fibrotic responses are inhibited by the PAR-2 specific antagonist. Targeting PAR-1 and PAR-2 simultaneously is not superior to targeting either receptor alone in bleomycin-induced pulmonary fibrosis. We postulate that the pro-fibrotic effects of PAR-1 require the presence of PAR-2. PMID:25689283

  12. Protease-activated receptor (PAR)-2 is required for PAR-1 signalling in pulmonary fibrosis

    PubMed Central

    Lin, Cong; von der Thüsen, Jan; Daalhuisen, Joost; ten Brink, Marieke; Crestani, Bruno; van der Poll, Tom; Borensztajn, Keren; Spek, C Arnold

    2015-01-01

    Idiopathic pulmonary fibrosis is the most devastating diffuse fibrosing lung disease of unknown aetiology. Compelling evidence suggests that both protease-activated receptor (PAR)-1 and PAR-2 participate in the development of pulmonary fibrosis. Previous studies have shown that bleomycin-induced lung fibrosis is diminished in both PAR-1 and PAR-2 deficient mice. We thus have been suggested that combined inactivation of PAR-1 and PAR-2 would be more effective in blocking pulmonary fibrosis. Human and murine fibroblasts were stimulated with PAR-1 and PAR-2 agonists in the absence or presence of specific PAR-1 or PAR-2 antagonists after which fibrotic markers like collagen and smooth muscle actin were analysed by Western blot. Pulmonary fibrosis was induced by intranasal instillation of bleomycin into wild-type and PAR-2 deficient mice with or without a specific PAR-1 antagonist (P1pal-12). Fibrosis was assessed by hydroxyproline quantification and (immuno)histochemical analysis. We show that specific PAR-1 and/or PAR-2 activating proteases induce fibroblast migration, differentiation and extracellular matrix production. Interestingly, however, combined activation of PAR-1 and PAR-2 did not show any additive effects on these pro-fibrotic responses. Strikingly, PAR-2 deficiency as well as pharmacological PAR-1 inhibition reduced bleomycin-induced pulmonary fibrosis to a similar extent. PAR-1 inhibition in PAR-2 deficient mice did not further diminish bleomycin-induced pulmonary fibrosis. Finally, we show that the PAR-1-dependent pro-fibrotic responses are inhibited by the PAR-2 specific antagonist. Targeting PAR-1 and PAR-2 simultaneously is not superior to targeting either receptor alone in bleomycin-induced pulmonary fibrosis. We postulate that the pro-fibrotic effects of PAR-1 require the presence of PAR-2. PMID:25689283

  13. Production of immunoactive inhibin by bovine granulosa cells in serum-free culture: effects of exogenous steroids and FSH.

    PubMed

    Wrathall, J H; Knight, P G

    1993-10-01

    Granulosa cells from pooled bovine follicles were cultured under chemically-defined (serum-free) conditions to study the effects of exogenous steroids and FSH on production of immunoactive (ia) inhibin, oestradiol and progesterone. Levels of ia-inhibin in media samples and cell lysates were measured by radioimmunoassay (RIA) using an antiserum raised against a synthetic fragment of human inhibin alpha-subunit [hI alpha (1-32)]. Cells secreted measurable amounts of ia-inhibin, oestradiol and progesterone for at least 7 d of culture, although intracellular levels of inhibin were very low, indicating that newly-synthesized ia-inhibin is rapidly released from the cells. Treatment with androstenedione (0.2 mumol/l) or testosterone (0.2 mumol/l) increased ia-inhibin secretion markedly; levels on Day 5 of culture were approximately 6-fold (P < 0.005) higher than control values. In contrast, treatment with the non-aromatizable androgen dihydrotestosterone (DHT; 0.2 mumol/l) resulted in only a one- to two-fold increase (P < 0.05) over control values (Day 5). Addition of exogenous oestradiol (8 nmol/l) markedly increased ia-inhibin secretion (8-9 fold on Day 5; P < 0.05) compared with basal levels, whereas progesterone had no effect. Secretion of oestradiol, undetectable in the absence of exogenous androgens, rose daily in the presence of either androstenedione or testosterone, levels rising approximately 6-fold and 9-fold respectively over a 4-d treatment period. Progesterone secretion increased approximately 2-fold over the culture period and was unaffected by any steroid treatment. Treatment with ovine FSH (10ng/ml) alone stimulated secretion of progesterone over basal levels (3-fold higher on Day 6; P < 0.005), but did not affect output of either ia-inhibin or oestradiol. However, exposure to FSH in the presence of androstenedione not only promoted a further 4-fold increase in progesterone output but also led to a dose-dependent suppression of both ia-inhibin (approximately 90% lower on Day 6; P < 0.001) and oestradiol (approximately 80% lower on Day 6; P < 0.001) secretion compared to cells treated with androstenedione alone. These observations indicate that the secretion of ia-inhibin by bovine granulosa cells in culture is positively regulated by oestradiol, implying an autocrine/paracrine role for this hormone in control of ovarian inhibin production. The ability of aromatizable androgens to stimulate secretion of inhibin, coupled with the inability of the non-aromatizable androgen DHT to elicit such an effect, suggests that inhibin output is largely unaffected by androgens prior to their conversion to oestradiol.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:8306633

  14. Exploration of locomotion in the ParA/ParB system

    NASA Astrophysics Data System (ADS)

    Jindal, Lavisha; Emberly, Eldon

    2015-03-01

    In many bacteria the ParA/ParB system is responsible for actively segregating DNA during replication. ParB precessively moves by hydrolyzing DNA bound ParA-ATP forming a depleted ParA region in its wake. Recent in-vitro experiments have shown that a ParB covered bead can traverse a ParA bound DNA substrate. It has been suggested that the formation of a gradient in ParA leads to diffusion-ratchet like motion of the ParB bead but its origin and potential consequences requires investigation. We have developed a deterministic model for the in-vitro ParA/ParB system and show that any amount of spatial noise in ParA can lead to the spontaneous formation of its gradient. The velocity of the bead is independent of this noise but depends on the scale over which ParA exerts a force on the bead and the scale over which ParB hydrolyzes ParA from the substrate. There is a particular ratio of these scales at which the velocity is a maximum. We also explore the effects of cooperative vs independent rebinding of ParA to the substrate. Our model shows how the driving force for ParB originates and highlights necessary conditions for directed motion in the in-vitro system that may provide insight into the in-vivo behaviour of the ParA/ParB system.

  15. Variations in LH and FSH serum gonadotropins in the hypophyseal chromophobe adenoma and acromegaly in the woman in relation to age.

    PubMed

    Piti?, M; E?anu, C; Klepsch, I; Grigorescu, A

    1978-01-01

    Investigation of the gonadotropic function by radioimmunoassay of serum LH and FSH in 62 women with hypophyseal chromophobe adenoma and acromegaly in relation to age and by comparison with 40 normal women revealed low values for LH and FSH during the reporduction period (20--43 years) and increased values in the postmenopausal period (48--84 years) like in physiologic postmenopause. The increase of the gonadotropic function in the hypophyseal tumoral syndrome of the chromophobe adenoma and acromegaly type above the age of 48 from low values compared to the normal to high values like those in physiologic menopause (a gonadotropic turning phenomenon) demonstrate an alteration of the hypothalamic receptors in relation to age and to these diseases. PMID:675127

  16. Signaling between the pituitary gland and the testes: inverse relationship between serum FSH and inhibin B concentrations in boys in early puberty

    Microsoft Academic Search

    Taneli Raivio; Sanna Saukkonen; Jarmo Jaaskelainen; Jorma Komulainen; Leo Dunkel

    2000-01-01

    Objectives: To study the relationship between serum inhibin B and sex steroid concentrations and pituitary FSH responsiveness to GnRH in boys in early puberty, and to examine serum inhibin B levels in prepubertal boys with different timing of the onset of gonadotropin deficiency (GD). Design: Twenty-five boys with constitutional delay of puberty (CDP; 20 in Tanner stage G2 and 5

  17. The Adapter Protein APPL1 Links FSH Receptor to Inositol 1,4,5-Trisphosphate Production and Is Implicated in Intracellular Ca2+ Mobilization

    PubMed Central

    Thomas, Richard M.; Nechamen, Cheryl A.; Mazurkiewicz, Joseph E.; Ulloa-Aguirre, Alfredo

    2011-01-01

    FSH binds to its receptor (FSHR) on target cells in the ovary and testis, to regulate oogenesis and spermatogenesis, respectively. The signaling cascades activated after ligand binding are extremely complex and have been shown to include protein kinase A, mitogen-activated protein kinase, phosphatidylinositol 3-kinase/protein kinase B, and inositol 1,4,5-trisphosphate–mediated calcium signaling pathways. The adapter protein APPL1 (Adapter protein containing Pleckstrin homology domain, Phosphotyrosine binding domain and Leucine zipper motif), which has been linked to an assortment of other signaling proteins, was previously identified as an interacting protein with FSHR. Thus, alanine substitution mutations in the first intracellular loop of FSHR were generated to determine which residues are essential for FSHR-APPL1 interaction. Three amino acids were essential; when any one of them was altered, APPL1 association with FSHR mutants was abrogated. Two of the mutants (L377A and F382A) that displayed poor cell-surface expression were not studied further. Substitution of FSHR-K376A did not affect FSH binding or agonist-stimulated cAMP production in either transiently transfected human embryonic kidney cells or virally transduced human granulosa cells (KGN). In the KGN line, as well as primary cultures of rat granulosa cells transduced with wild type or mutant receptor, FSH-mediated progesterone or estradiol production was not affected by the mutation. However, in human embryonic kidney cells inositol 1,4,5-trisphosphate production was curtailed and KGN cells transduced with FSHR-K376A evidenced reduced Ca2+ mobilization from intracellular stores after FSH treatment. PMID:21285318

  18. Gene expression differences induced by equimolar low doses of LH or hCG in combination with FSH in cultured mouse antral follicles.

    PubMed

    Segers, Ingrid; Adriaenssens, Tom; Wathlet, Sandra; Smitz, Johan

    2012-11-01

    In a natural cycle, follicle growth is coordinated by FSH and LH. Follicle growth stimulation in Assisted Reproductive Technologies (ART) requires antral follicles to be exposed to both FSH and LH bioactivity, especially after GNRH analog pretreatment. The main aim was to detect possible differences in gene expression in granulosa cells after exposing the follicle during antral growth to LH or hCG, as LH and hCG are different molecules acting on the same receptor. Effects of five gonadotropin treatments were investigated for 16 genes using a mouse follicle culture model. Early (day 6) antral follicles were exposed to high recombinant FSH combined or not with equimolar concentrations of recombinant LH (rLH) or recombinant hCG (rhCG) and to highly purified human menopausal gonadotropin (HP-hMG) for 6?h, 12?h, or 3 days. Expression differences were tested for genes involved in steroidogenesis: Mvk, Lss, Cyp11a1, Hsd3b1, Cyp19a1, Nr4a1, and Timp1; final granulosa differentiation: Lhcgr, Oxtr, Pgr, Egfr, Hif1a, and Vegfa; and cytokines: Cxcl12, Cxcr4, and Sdc4. Lhcgr was present and upregulated by gonadotropins. Nr4a1, Cxcl12, and Cxcr4 showed a different expression pattern if LH bioactivity was added to high FSH in the first hours after exposure. However, no signs of premature luteinization were present even after a 3-day treatment as shown by Cyp19a1, Oxtr, Pgr, and Egfr and by estrogen and progesterone measurements. The downstream signaling by rhCG or rLH through the LHCGR was not different for this gene selection. Granulosa cells from follicles exposed to HP-hMG showed an enhanced expression level for several genes compared with recombinant gonadotropin exposure, possibly pointing to enhanced cellular activity. PMID:22906696

  19. Oocytes lacking O-glycans alter follicle development and increase fertility by increasing follicle FSH sensitivity, decreasing apoptosis, and modifying GDF9:BMP15 expression.

    PubMed

    Grasa, Patricia; Ploutarchou, Panayiota; Williams, Suzannah A

    2015-02-01

    The number of eggs ovulated varies within and between species and is influenced by many variables. However, the regulatory mechanisms remain poorly understood. We previously demonstrated a key role for the oocyte because mice generating oocytes deficient in core 1-derived O-glycans ovulate ?40-50% more eggs than Controls. Here we analyze the basis of this phenotype using Mutant [core 1 ?1,3-galactosyltransferase 1 (C1galt1)(FF):zona pellucida glycoprotein 3 Cre (ZP3Cre)] and Control (C1galt1(FF)) female mice. In culture, Mutant follicles exhibited delayed antrum formation [indicative of follicle stimulant hormone (FSH) dependence] and increased sensitivity to FSH. Although the Mutant estrous cycle was extended, comprehensive endocrine changes were not observed; rather FSH, LH, inhibin B, and anti-Mullerian hormone were temporally altered, revealing estrous cycle stage-specific modifications to the hypothalamic-pituitary-gonadal axis. At proestrus, when FSH levels were decreased in Mutants, ovaries contained more, smaller, preantral follicles. Mutant follicles exhibited reduced levels of apoptosis, and both B-cell lymphoma 2 (Bcl-2) and BCL-2-associated X protein (Bax) were altered compared with Controls. Mutant ovaries also had an increase in the expression ratio of growth differentiation factor 9 (GDF9):bone morphogenetic protein 15 (BMP15) at diestrus. On the basis of these data, we propose that modified oocyte glycoproteins alter GDF9:BMP15 expression modifying follicle development resulting in the generation of more follicles. Thus, the oocyte is a key regulator of follicle development and has a crucial role in determining ovulation rate. PMID:25416550

  20. Mapping of a discontinuous and highly conformational binding site on follicle stimulating hormone subunit-? (FSH-?) using domain Scan™ and Matrix Scan™ technology

    Microsoft Academic Search

    Peter Timmerman; Evert Van Dijk; Wouter Puijk; Wim Schaaper; Jerry Slootstra; Stephen J. Carlisle; John Coley; Steve Eida; M. Gani; Tim Hunt; Paul Perry; Gerry Piron; Rob H. Meloen

    2004-01-01

    This paper describes the application of two novel screening technologies, i.e. Domain Scan (24- and 30-mer peptides) and Matrix Scan (24-mer peptides)technology, in the mapping of a discontinuous epitope on FSH- for a series of 20 monoclonal antibodies. 11 out of 20 mAb's, mapping of which was not successful by conventional Pepscan technology (12-merpeptides), showed selective binding to peptide-constructs corresponding

  1. Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes

    Microsoft Academic Search

    Akio Shimizu; Hideki Tanaka; Hirohiko Kagawa

    2003-01-01

    Two distinct types of gonadotrophs, FSH (GtH I) cells and LH (GtH II) cells, were immunocytochemically identified from mummichog (Fundulus heteroclitus; Cyprinodontiformes, Acanthopterygii) pituitary using antisera raised against synthetic fragment peptides of FSH? and LH?. Both cell types were abundant during the spawning period (spring and early summer) and decreased in number during the post-spawning immature period. The number of

  2. The ParB-parS Chromosome Segregation System Modulates Competence Development in Streptococcus pneumoniae

    PubMed Central

    Attaiech, Laetitia; Minnen, Anita; Kjos, Morten; Gruber, Stephan

    2015-01-01

    ABSTRACT ParB proteins bind centromere-like DNA sequences called parS sites and are involved in plasmid and chromosome segregation in bacteria. We previously showed that the opportunistic human pathogen Streptococcus pneumoniae contains four parS sequences located close to the origin of replication which are bound by ParB. Using chromatin immunoprecipitation (ChIP), we found here that ParB spreads out from one of these parS sites, parS(?1.6°), for more than 5 kb and occupies the nearby comCDE operon, which drives competence development. Competence allows S. pneumoniae to take up DNA from its environment, thereby mediating horizontal gene transfer, and is also employed as a general stress response. Mutating parS(?1.6°) or deleting parB resulted in transcriptional up-regulation of comCDE and ssbB (a gene belonging to the competence regulon), demonstrating that ParB acts as a repressor of competence. However, genome-wide transcription analysis showed that ParB is not a global transcriptional regulator. Different factors, such as the composition of the growth medium and antibiotic-induced stress, can trigger the sensitive switch driving competence. This work shows that the ParB-parS chromosome segregation machinery also influences this developmental process. PMID:26126852

  3. Granulosa Cell-Specific Brca1 Loss Alone or Combined with Trp53 Haploinsufficiency and Transgenic FSH Expression Fails to Induce Ovarian Tumors.

    PubMed

    Upton, Dannielle H; Fuller, Emily S; Colvin, Emily K; Walters, Kirsty A; Jimenez, Mark; Desai, Reena; Handelsman, David J; Howell, Viive M; Allan, Charles M

    2015-08-01

    BRCA1 mutations are associated with ovarian cancer. Previous studies reported that murine granulosa cell (GC) Brca1 loss caused ovarian-uterine tumors resembling serous cystadenomas, but the pathogenesis of these tumors may have been confounded by ectopic Brca1 expression and altered estrous cycling. We have used Tg.AMH.Cre conferring proven ovarian and GC-specific Cre activity to selectively target Brca1 disruption, denoted Brca1 (GC-/-). Furthermore, ovary-specific Brca1 (GC-/-) was combined with global Trp53 haploinsufficiency (Trp53 (+/-)) and transgenic follicle-stimulating hormone (Tg.FSH) overexpression as a multi-hit strategy to investigate additional genetic and hormonal ovarian tumorigenesis mechanisms. However, 12-month-old Brca1 (GC-/-) mice had no detectable ovarian or uterine tumors. Brca1 (GC-/-) mice had significantly increased ovary weights, follicles exhibiting more pyknotic granulosa cells, and fewer corpora lutea with regular estrous cycling compared to controls. Isolated Brca1 (GC-/-) mutation lengthened the estrous cycle and proestrus stage; however, ovarian cystadenomas were not observed, even when Brca1 (GC-/-) was combined with Trp53 (+/-) and overexpressed Tg.FSH. Our Brca1 (GC-/-) models reveal that specific intra-follicular Brca1 loss alone, or combined with cancer-promoting genetic (Trp53 loss) and endocrine (high serum FSH) changes, was not sufficient to cause ovarian tumors. Our findings show that the ovary is remarkably resistant to oncogenesis, and support the emerging view of an extragonadal, multi-hit origin for ovarian tumorigenesis. PMID:25943777

  4. Short follicular phase of stimulation following corifollitropin alfa or daily recombinant FSH treatment does not compromise clinical outcome: a retrospective analysis of the Engage trial.

    PubMed

    Mardeši?, Tonko; Mannaerts, Bernadette; Abuzeid, Mostafa; Levy, Michael; Witjes, Han; Fauser, Bart C J M

    2014-04-01

    To evaluate whether a short follicular phase of ovarian stimulation compromises the chance of pregnancy, subjects from a double-blind, randomized trial treated with a single dose of corifollitropin alfa (n=756) or daily recombinant FSH (n=750) were categorized as early responders if three follicles ?17 mm were reached and human chorionic gonadotrophin (HCG) was administered prior to or on stimulation day 8, and as normal responders if three follicles ?17 mm were reached and HCG was administered after stimulation day 8. In the corifollitropin alfa and recombinant FSH groups, 23.2% and 29.1%, respectively, were early responders (P=0.01). Regardless of the treatment group, the initial ovarian response was higher in early responders, but with two extra days of stimulation, the number and size of follicles on the day of HCG in the normal responders was similar to those of the early responders. The number of oocytes was similar in both response groups following corifollitropin alfa treatment (13.6 versus 14.5) and recombinant FSH treatment (12.8, both groups). The ongoing pregnancy rates were comparable for early and normal responders regardless of the treatment group, supporting successful outcome following a stimulation period of only 1 week. PMID:24581989

  5. Effect of immunization of rams against bovine inhibin alpha 1-26 on semen characteristics, scrotal size, FSH, LH and testosterone concentrations.

    PubMed

    McKeown, R M; O'Callaghan, D; Roche, J F; Boland, M P

    1997-03-01

    The effects of inhibin immunization on inhibin antibody titres, semen characteristics, scrotal size, fertility, FSH, LH and testosterone concentrations were determined by immunizing adult rams against bovine inhibin alpha 1-26-Gly-Tyr conjugated to human serum albumin (n = 16) in non-ulcerative Freund's adjuvant and DEAE:dextran (1:1) or adjuvant alone (n = 16) on days 0 (29 June), 30, 60, 191, 303 and 394. Blood samples were collected and bovine inhibin alpha 1-26-Gly-Tyr antibody titres and serum testosterone concentrations were determined. Each month, between days 174 and 417, semen was collected every 30 min to a maximum of 15 ejaculates over 7 h and scrotal circumference was measured. Ram fertility was recorded during natural service. FSH, LH and testosterone concentrations and GnRH-induced FSH and LH release were measured in a subgroup of immunized (n = 5) and control (n = 5) rams at frequent intervals. Antibody titres were variable among immunized rams (0-46% 125I-labelled bovine inhibin alpha 1-26-Gly-Tyr at 1:1600 serum dilution) but mean titres were consistently higher than in control rams (P < or = 0.001). Immunization did not alter the semen volume, output or quality of spermatozoa or ram fertility, but increased the mean scrotal circumference (37.6 +/- 0.8 cm versus 34.4 +/- 0.7 cm, P < 0.001). Mean FSH concentrations were higher in immunized rams during two intensive blood sampling periods (in June and August) (5.8 +/- 0.7 ng ml-1 versus 3.0 +/- 0.3 ng ml-1, P < 0.001 in June; and 4.8 +/- 0.9 ng ml-1 versus 2.0 +/- 0.3 ng ml-1, P < 0.02 in August), and were correlated with antibody titres (r2 = 0.3, P < 0.05 in June; and r2 = 0.8, P < 0.001 in August). Discrete FSH pulses were not detected. Immunization did not alter mean or basal testosterone or LH concentrations, or LH pulse frequency; LH pulse amplitude was increased (1.6 +/- 0.2 ng ml-1 versus 0.8 +/- 0.2 ng ml-1, P < 0.02) and was correlated with antibody titres (r2 = 0.6, P < 0.01). Immunization enhanced GnRH-induced FSH (P < 0.05) but not LH release. In conclusion, immunization of adult rams against bovine inhibin alpha 1-26 Gly-Tyr increased scrotal circumference, mean FSH concentrations and LH pulse amplitude, without altering semen characteristics, fertility, mean LH concentrations, LH pulse frequency or mean testosterone concentrations. PMID:9155733

  6. Prsente par Adel EL OMRI

    E-print Network

    Paris-Sud XI, Université de

    THESE Présentée par Adel EL OMRI Pour l'obtention du GRADE DE DOCTEUR Spécialité : Génie Industriel, this dissertation would not be possible. Adel tel-00453322,version1-4Feb2010 #12;tel-00453322,version1-4Feb2010 #12

  7. Mmoire prsent par Franois Renard

    E-print Network

    Mémoire présenté par François Renard Laboratoire de Géophysique Interne et Tectonophysique presented by François Renard Laboratoire de Géophysique Interne et Tectonophysique Observatoire des Sciences mécaniques. D'autre part de nouveaux thèmes de recherche ont été initiés: étude expérimentale du frottement

  8. A critical role of follicle-stimulating hormone (Fsh) in mediating the effect of clotrimazole on testicular steroidogenesis in adult zebrafish.

    PubMed

    Baudiffier, Damien; Hinfray, Nathalie; Vosges, Mélanie; Creusot, Nicolas; Chadili, Edith; Porcher, Jean-Marc; Schulz, Rüdiger W; Brion, François

    2012-08-16

    Clotrimazole is a pharmaceutical fungicide known to inhibit several cytochrome P450 enzyme activities, including several steroidogenic enzymes. This study aimed to assess short-term in vivo effects of clotrimazole exposure on blood 11-ketotestosterone (11-KT) levels and on the transcriptional activity of genes in pituitary and testis tissue that are functionally relevant for androgen production with the view to further characterize the mode of action of clotrimazole on the hypothalamus-pituitary-gonad axis in zebrafish, a model vertebrate in toxicology. Adult male zebrafish were exposed to measured concentrations in water of 71, 159 and 258?g/L of clotrimazole for 7 days. Expression of pituitary gonadotropins ? subunit (lhb, fshb), testicular gonadotropins receptors (lhcgr, fshr) and testicular steroidogenesis-related genes (e.g., star, cyp17a1, cyp11c1) were assessed. Blood concentrations of 11-KT were measured. Short-term exposure to clotrimazole induced a concentration-dependent increase of star, cyp17a1, and cyp11c1 gene expression and Cyp17a1 and Cy11c1 protein synthesis in Leydig cells, but androgen levels in blood remained unchanged. fshb, but not lhb mRNA levels in the pituitary tended to increase in clotrimazole-exposed zebrafish. Testicular expression of the Fsh receptor gene was significantly up-regulated following exposure, when expression of this receptor was significantly correlated to the expression of steroidogenesis-related genes. Moreover, the Fsh-regulated insulin-like growth factor 3 (igf3) gene, a fish-specific Igf peptide expressed in Sertoli cells, was induced in testes. By using a network of genes functioning in pituitary and testis tissue, our study demonstrated that clotrimazole induced a cascade of molecular and cellular events which are in agreement with a role for Fsh (1) in stimulating Leydig cell steroidogenesis to compensate the inhibitory action of clotrimazole on 11-KT synthesis and (2) in inducing the expression of Fsh-regulated igf3 in Sertoli cells. PMID:22564764

  9. ParB spreading requires DNA bridging

    PubMed Central

    Graham, Thomas G.W.; Wang, Xindan; Song, Dan; Etson, Candice M.; van Oijen, Antoine M.; Rudner, David Z.; Loparo, Joseph J.

    2014-01-01

    The parABS system is a widely employed mechanism for plasmid partitioning and chromosome segregation in bacteria. ParB binds to parS sites on plasmids and chromosomes and associates with broad regions of adjacent DNA, a phenomenon known as spreading. Although essential for ParB function, the mechanism of spreading remains poorly understood. Using single-molecule approaches, we discovered that Bacillus subtilis ParB (Spo0J) is able to trap DNA loops. Point mutants in Spo0J that disrupt DNA bridging are defective in spreading and recruitment of structural maintenance of chromosomes (SMC) condensin complexes in vivo. DNA bridging helps to explain how a limited number of Spo0J molecules per parS site (?20) can spread over many kilobases and suggests a mechanism by which ParB proteins could facilitate the loading of SMC complexes. We show that DNA bridging is a property of diverse ParB homologs, suggesting broad evolutionary conservation. PMID:24829297

  10. ParAB Partition Dynamics in Firmicutes: Nucleoid Bound ParA Captures and Tethers ParB-Plasmid Complexes

    PubMed Central

    Lioy, Virginia S.; Volante, Andrea; Soberón, Nora E.; Lurz, Rudi; Ayora, Silvia; Alonso, Juan C.

    2015-01-01

    In Firmicutes, small homodimeric ParA-like (?2) and ParB-like (?2) proteins, in concert with cis-acting plasmid-borne parS and the host chromosome, secure stable plasmid inheritance in a growing bacterial population. This study shows that (?:YFP)2 binding to parS facilitates plasmid clustering in the cytosol. (?:GFP)2 requires ATP binding but not hydrolysis to localize onto the cell’s nucleoid as a fluorescent cloud. The interaction of (?:CFP)2 or ?2 bound to the nucleoid with (?:YFP)2 foci facilitates plasmid capture, from a very broad distribution, towards the nucleoid and plasmid pairing. parS-bound ?2 promotes redistribution of (?:GFP)2, leading to the dynamic release of (?:GFP)2 from the nucleoid, in a process favored by ATP hydrolysis and protein-protein interaction. (?D60A:GFP)2, which binds but cannot hydrolyze ATP, also forms unstable complexes on the nucleoid. In the presence of ?2, (?D60A:GFP)2 accumulates foci or patched structures on the nucleoid. We propose that (?:GFP)2 binding to different nucleoid regions and to ?2-parS might generate (?:GFP)2 gradients that could direct plasmid movement. The iterative pairing and unpairing cycles may tether plasmids equidistantly on the nucleoid to ensure faithful plasmid segregation by a mechanism compatible with the diffusion-ratchet mechanism as proposed from in vitro reconstituted systems. PMID:26161642

  11. Seasonal expression of KiSS-1 and the pituitary gonadotropins LH? and FSH? in adult male Libyan jird (Meriones libycus).

    PubMed

    Boufermes, R; Richard, N; Le Moguen, K; Amirat, Z; Khammar, F; Kottler, M L

    2014-06-10

    The molecular mechanisms operating on a seasonal time-scale and regulating functions such as reproduction are poorly understood in animals living in desert environments. Kisspeptin, the product of the KiSS-1 gene, plays a critical role in control of the hypothalamic-pituitary-gonad axis that orchestrates the reproductive system in vertebrates. We report a sequence analysis of KiSS-1 and the pituitary luteinising hormone-beta (LH?) and follicle-stimulating hormone-beta (FSH?) in the Libyan jird (Meriones libycus), a seasonal breeding rodent that is sexually active during spring and quiescent in fall. We also assessed gene expression by quantitative real-time reverse transcription-polymerase chain reaction during the non-breeding and breeding seasons. The KiSS-1 cDNA sequence analysis showed high homology between M. libycus and all other rodents (94%) and humans (92%). KiSS-1 expression was higher during the breeding season than that during the non-breeding season. In contrast, LH? and FSH? expression levels were higher during the non-breeding season in autumn and varied in an opposite manner with testicular, seminal vesicle weights and plasma testosterone levels. Our results extend the role for KiSS-1 in activating the HPG axis in this desert rodent in its natural biotope by relaying environmental cues as in other seasonal non-desert rodent models. PMID:24786546

  12. Elaborating polarity: PAR proteins and the cytoskeleton

    PubMed Central

    Nance, Jeremy; Zallen, Jennifer A.

    2011-01-01

    Cell polarity is essential for cells to divide asymmetrically, form spatially restricted subcellular structures and participate in three-dimensional multicellular organization. PAR proteins are conserved polarity regulators that function by generating cortical landmarks that establish dynamic asymmetries in the distribution of effector proteins. Here, we review recent findings on the role of PAR proteins in cell polarity in C. elegans and Drosophila, and emphasize the links that exist between PAR networks and cytoskeletal proteins that both regulate PAR protein localization and act as downstream effectors to elaborate polarity within the cell. PMID:21303844

  13. PAR and Supply Distribution System The PAR Program is administered by Materials Logistics (Central Distribution)

    E-print Network

    Oliver, Douglas L.

    Logistics (Central Distribution) Services, a division of Materials Management. · The PAR Program is focused#12;PAR and Supply Distribution System OVERVIEW · The PAR Program is administered by Materials of completing the services. · Provide supply distribution and replenishment services that met the needs

  14. PRODUCTION D'OSCILLATIONS PAR LES DIODES A EFFET TUNNEL Par REN MARCHANDEAU,

    E-print Network

    Paris-Sud XI, Université de

    101 A. PRODUCTION D'OSCILLATIONS PAR LES DIODES A EFFET TUNNEL Par RENÉ MARCHANDEAU, Laboratoire d'oscillations par une diode à effet tunnel, dans un circuit résonnant parallèle, est étudiée au moyen d'une méthode The generation of oscillations by a tunnel diode in a parallel tuned circuit is studied by means of a graphical

  15. MTHODE DE RALISATION DE COUCHES MINCES DE CdTe PAR TRANSPORT GAZEUX Par C. PIAGET,

    E-print Network

    Paris-Sud XI, Université de

    201. MÉTHODE DE RÉALISATION DE COUCHES MINCES DE CdTe PAR TRANSPORT GAZEUX Par C. PIAGET dépôt de couches minces de CdTe (transport par l'iode et évaporation sous vide) sont transposées des basses pressions à des pressions voisines de l'atmosphère. La dissociation et la cristallisation du CdTe

  16. PAR for the Course: A Congruent Pedagogical Approach for a PAR Methods Class

    ERIC Educational Resources Information Center

    Hammond, Joyce D.; Hicks, Maria; Kalman, Rowenn; Miller, Jason

    2005-01-01

    In the past two years, three graduate students and a senior faculty member have co-taught a participatory action research (PAR) course to undergraduate and graduate students. In this article the co-teachers advocate a set of pedagogical principles and practices in a PAR-oriented classroom that establishes congruency with community PAR projects in…

  17. COLLIMATION DU RAYONNEMENT GAMMA PAR UN CANAL CYLINDRIQUE CIRCULAIRE Par C. KELLERSHOHN et P. PELLERIN,

    E-print Network

    Paris-Sud XI, Université de

    81 A COLLIMATION DU RAYONNEMENT GAMMA PAR UN CANAL CYLINDRIQUE CIRCULAIRE Par C. KELLERSHOHN et P'apprécier le degré de collimation d'un rayonnement gamma par un canal cylindrique dans un matériau absorbant. Application au cas de la raie 364 KeV de 131I et d'un canal à parois de plomb. LE JOURNAL DE PHYSIQUE ET LE

  18. Interaction of thrombin with PAR1 and PAR4 at the thrombin cleavage site†

    PubMed Central

    Nieman, Marvin T.; Schmaier, Alvin H.

    2008-01-01

    Investigations determined the critical amino acids for ?-thrombin’s interaction with protease activated receptors 1 and 4 (PAR1 and PAR4) at the thrombin cleavage site. Recombinant PAR1-wild type (wt) exodomain was cleaved by ?-thrombin with a Km of 28 ?M, a kcat of 340 s-1 and kcat/Km of 1.2×107. When the P4 or P2 position was mutated to alanine, PAR1-L38A or PAR1-P40A, respectively, the Km was unchanged, 29 or 23 ?M, respectively; however the kcat and kcat/Km were reduced in each case. In contrast, when Asp39 at P3 was mutated to alanine, PAR1-D39A, the Km and kcat were both reduced ~3-fold making the kcat/Km the same as PAR1-wt exodomain. Recombinant PAR4-wt exodomain was cleaved by ?-thrombin with a Km of 61 ?M, a kcat of 17 s-1 and kcat/Km of 2.8 ×105. When the P5 or P4 position was mutated to alanine PAR4-L43A or PAR4-P44A, respectively, there was no change in the Km (69 or 56 ?M, respectively) however; the kcat was lowered in each case (9.7 or 7.7 s-1, respectively). Mutation of the P2 position (PAR4-P46A) also had no effect on the Km but markedly lowered the kcat and kcat/Km ~35-fold. PAR1-wt exodomain and P4 and P3 mutants were noncompetitive inhibitors of ?-thrombin hydrolyzing Sar-Pro-Arg-pNA. However, PAR1-P40A displayed mixed type of inhibition. Mutation of P4, P3 or P2 had no effect on the Ki. All PAR4 exodomains were competitive inhibitors of ?-thrombin. Mutation of P5, P4 or P2 had no effect on the Ki. These investigations show that Leu at P4 in PAR1 or P5 in PAR4 critically influences the kinetics of ?-thrombin binding and cleavage of PAR1 and PAR4 exodomains. It also implies that factors other than the hirudin-like binding region on PAR1 exodomain predominate in influencing PAR1 cleavage on cells. PMID:17595115

  19. Par Pond vegetation status Summer 1995 -- Summary

    SciTech Connect

    Mackey, H.E. Jr.; Riley, R.S.

    1996-01-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the newly emergent, shoreline aquatic plant communities of Par Pond began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level. These surveys continued in July, September, and late October, 1995. Communities similar to the pre-drawdown, Par Pond aquatic plant communities are becoming re-established. Emergent beds of maidencane, lotus, waterlily, and watershield are extensive and well developed. Cattail occurrence continued to increase during the summer, but large beds common to Par Pond prior to the drawdown have not formed. Estimates from SPOT HRV, remote sensing satellite data indicated that as much as 120 hectares of emergent wetlands vegetation may have been present along the Par Pond shoreline by early October, 1995. To track the continued development of macrophytes in Par Pond, future surveys throughout 1996 and 1997, along with the continued evaluation of satellite data to map the areal extent of the macrophyte beds of Par Pond, are planned.

  20. Par Aziz EL TATAR Thse prsente

    E-print Network

    Paris-Sud XI, Université de

    les signaux après leur contamination par les artéfacts générés par les séquences d'imagerie. Il. We developed a device to allow a deep study of the contamination mechanism, which would assist, via an optical cable, the collected signal to a receiver box placed outside the MRI room. The receiver

  1. Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes.

    PubMed

    Shimizu, Akio; Tanaka, Hideki; Kagawa, Hirohiko

    2003-06-01

    Two distinct types of gonadotrophs, FSH (GtH I) cells and LH (GtH II) cells, were immunocytochemically identified from mummichog (Fundulus heteroclitus; Cyprinodontiformes, Acanthopterygii) pituitary using antisera raised against synthetic fragment peptides of FSHbeta and LHbeta. Both cell types were abundant during the spawning period (spring and early summer) and decreased in number during the post-spawning immature period. The number of FSH cells increased again during the early phases of gonadal development (cortical alveoli accumulation in the oocytes and basal spermatogenesis) in early winter, whereas the number of LH cells did not. Only FSH cells were abundant during the latter phases of gonadal development (vitellogenesis and active spermatogenesis) in early spring. These observations suggest that both GtHs have important yet different roles for reproduction in this species. Antisera against the conservative region of the FSHbeta and the LHbeta subunits immunostained FSH cells and LH cells, respectively, also in red seabream (Pagrus major; Perciformes, Acanthopterygii) and small mouth bass (Micropterus dolomieu; Perciformes, Acanthopterygii), suggesting the possibility of their use for other acanthopterygian fishes. PMID:12765642

  2. Reproductive Physiology in Young Men Is Cumulatively Affected by FSH-Action Modulating Genetic Variants: FSHR -29G/A and c.2039 A/G, FSHB -211G/T

    PubMed Central

    Grigorova, Marina; Punab, Margus; Punab, Anna Maria; Poolamets, Olev; Vihljajev, Vladimir; Žilaitien?, Birut?; Erenpreiss, Juris; Matulevi?ius, Valentinas; Laan, Maris

    2014-01-01

    Follicle-Stimulating Hormone Receptor (FSHR) -29G/A polymorphism (rs1394205) was reported to modulate gene expression and reproductive parameters in women, but data in men is limited. We aimed to bring evidence to the effect of FSHR -29G/A variants in men. In Baltic young male cohort (n?=?982; Estonians, Latvians, Lithuanians; aged 20.2±2.0 years), the FSHR -29 A-allele was significantly associated with higher serum FSH (linear regression: effect 0.27 IU/L; P?=?0.0019, resistant to Bonferroni correction for multiple testing) and showed a non-significant trend for association with higher LH (0.19 IU/L) and total testosterone (0.93 nmol/L), but reduced Inhibin B (?7.84 pg/mL) and total testes volume (effect ?1.00 mL). Next, we extended the study and tested the effect of FSHR gene haplotypes determined by the allelic combination of FSHR -29G/A and a well-studied variant c.2039 A/G (Asn680Ser, exon 10). Among the FSHR -29A/2039G haplotype carriers (A-Ser; haplotype-based linear regression), this genetic effect was enhanced for FSH (effect 0.40 IU/L), Inhibin B (?16.57 pg/mL) and total testes volume (?2.34 mL). Finally, we estimated the total contribution of three known FSH-action modulating SNPs (FSHB -211G/T; FSHR -29G/A, c.2039 A/G) to phenotypic variance in reproductive parameters among young men. The major FSH-action modulating SNPs explained together 2.3%, 1.4%, 1.0 and 1.1% of the measured variance in serum FSH, Inhibin B, testosterone and total testes volume, respectively. In contrast to the young male cohort, neither FSHR -29G/A nor FSHR haplotypes appeared to systematically modulate the reproductive physiology of oligozoospermic idiopathic infertile patients (n?=?641, Estonians; aged 31.5±6.0 years). In summary, this is the first study showing the significant effect of FSHR -29G/A on male serum FSH level. To account for the genetic effect of known common polymorphisms modulating FSH-action, we suggest haplotype-based analysis of FSHR SNPs (FSHR -29G/A, c.2039 A/G) in combination with FSHB -211G/T testing. PMID:24718625

  3. AGirenPArTenAriAT despartenariats

    E-print Network

    la gestion des écosystèmes majeurs de la région1 . Le séminaire Les intérieurs du Maroc a par'Institut national de recherche halieutique au Maroc,l'Écolenationaled'ingénieursdeTunisoul'Institutnatio- nal

  4. Faits et phnomnes tudis par les OHM

    E-print Network

    van Tiggelen, Bart

    Faits et phénomènes étudiés par les OHM Anthropisation des écosystèmes.· Dynamiques de la biodiversité.· Dynamiques des pollutions et écotoxicologie.· Environnement et santé.· Modification des occupations humaines et impacts, résiliences· et ruptures, dégradations et réhabilitations. Adaptation aux

  5. Memoire de MASTER RECHERCHE presente par

    E-print Network

    Paris-Sud XI, Université de

    'augmentation de fr´equence allant de pair. Bien que la finesse de gravure continue de progresser, r´eduisant de composants, en utilisant notamment l'espace offert par une gravure plus fine pour ajou- ter des unit

  6. NOUVEAU SYSTME TLGRAPHIQUE; PAR M. ESTIENNE.

    E-print Network

    Paris-Sud XI, Université de

    conformément au code Morse. Le trait peut occuper, en hauteur, la largeur du ruban de papier, quelle que soit volume du Morse. L'avan- cement du papier se produit par un mécanisme analogue à celui de ce dernier

  7. Simulink/PARS Integration Support

    SciTech Connect

    Vacaliuc, B. [ORNL] [ORNL; Nakhaee, N. [Sundance DSP, Inc.] [Sundance DSP, Inc.

    2013-12-18

    The state of the art for signal processor hardware has far out-paced the development tools for placing applications on that hardware. In addition, signal processors are available in a variety of architectures, each uniquely capable of handling specific types of signal processing efficiently. With these processors becoming smaller and demanding less power, it has become possible to group multiple processors, a heterogeneous set of processors, into single systems. Different portions of the desired problem set can be assigned to different processor types as appropriate. As software development tools do not keep pace with these processors, especially when multiple processors of different types are used, a method is needed to enable software code portability among multiple processors and multiple types of processors along with their respective software environments. Sundance DSP, Inc. has developed a software toolkit called “PARS”, whose objective is to provide a framework that uses suites of tools provided by different vendors, along with modeling tools and a real time operating system, to build an application that spans different processor types. The software language used to express the behavior of the system is a very high level modeling language, “Simulink”, a MathWorks product. ORNL has used this toolkit to effectively implement several deliverables. This CRADA describes this collaboration between ORNL and Sundance DSP, Inc.

  8. SUR UN NOUVEAU RHOSTAT; PAR M. CROVA.

    E-print Network

    Boyer, Edmond

    du rhéostat. Pour se servir à volonté d'un seul fil, et pour pouvoir les étudier séparément, on peut table de leurs résistances en divers points de lcur longueur. Enfin on peut encore se servir du rhéostat conducteur de résistance négligeable, et sort par la borne inférieure p. On peut se servir ainsi de trois

  9. Radioecological implications of the Par Pond drawdown

    SciTech Connect

    Hickey, H. [Westinghouse Savannah River Co., Aiken, SC (United States); Whicker, F.W. [Savannah River Ecology Lab., Aiken, SC (United States)

    1991-12-05

    The drawdown of the Par Pond reservoir created dramatic alterations in this formerly stable lentic ecosystem. In addition, the radiation environment at Par Pond has changed significantly because of the exposure of Cesium 137-contaminated sediments and the appearance of new transport pathways to the terrestrial environment. In response to this situation, SREL was asked to study the radioecological implications of the reservoir drawdown. This report contains the objectives, methods, and results of the SREL study.

  10. Céphalées par abus médicamenteux et addiction

    Microsoft Academic Search

    F. Radat; C. Creac'h; M. Lafitau; S. Irachabal; V. Dousset; P. Henry

    2005-01-01

    Résumé  \\u000a Objectifs: étude de la comorbidité pour les troubles liés à l’utilisation de substances psychoactives chez les patients souffrant de\\u000a céphalées par abus médicamenteux.Méthode: étude comparative de quarante et un migraineux (MIG) et quarante et un patients souffrant de céphalées par abus médicamenteux\\u000a issus de migraines (CAM).Résultats: les CAM ulilisant des médicaments contenant des substances psychoactives (caféine et codéine) remplissent

  11. Exploration de r eseaux par un robot

    E-print Network

    Ilcinkas, David

    Exploration de r â?? eseaux par un robot (Network exploration with a robot) P. Fraigniaud 1 , D Râ?? esumâ?? e. Cet article traite de l'exploration d'un râ??eseau par un agent mobile, ou robot, mod d'au plus K+1 noeuds que le robot ne râ??eussit pas â?? a explorer. Cette borne K+1 amâ??eliore toutes les

  12. Maladies émergentes transmises par les tiques

    Microsoft Academic Search

    Ingomar Mutz

    2009-01-01

    RésuméLes infections transmises par les tiques surviennent dans le monde entier et sont bien connues depuis plus d’un siècle. Certaines d’entre elles sont très courantes alors que d’autres sont extrêmement rares. Les techniques modernes de génétique moléculaire (dont une plus grande disponibilité de la réaction en chaîne par polymérase (PCR) (de l’anglais polymerase chain reaction) ont permis la découverte et

  13. Protease-Activated Receptor (PAR) 1 and PAR4 Differentially Regulate Factor V Expression from Human Platelets

    PubMed Central

    Duvernay, Matthew; Young, Summer; Gailani, David; Schoenecker, Jonathan

    2013-01-01

    With the recent interest of protease-activated receptors (PAR) 1 and PAR4 as possible targets for the treatment of thrombotic disorders, we compared the efficacy of protease-activated receptor (PAR)1 and PAR4 in the generation of procoagulant phenotypes on platelet membranes. PAR4-activating peptide (AP)–stimulated platelets promoted thrombin generation in plasma up to 5 minutes earlier than PAR1-AP–stimulated platelets. PAR4-AP–mediated factor V (FV) association with the platelet surface was 1.6-fold greater than for PAR1-AP. Moreover, PAR4 stimulation resulted in a 3-fold greater release of microparticles, compared with PAR1 stimulation. More robust FV secretion and microparticle generation with PAR4-AP was attributable to stronger and more sustained phosphorylation of myosin light chain at serine 19 and threonine 18. Inhibition of Rho-kinase reduced PAR4-AP–mediated FV secretion and microparticle generation to PAR1-AP–mediated levels. Thrombin generation assays measuring prothrombinase complex activity demonstrated 1.5-fold higher peak thrombin levels on PAR4-AP–stimulated platelets, compared with PAR1-AP–stimulated platelets. Rho-kinase inhibition reduced PAR4-AP–mediated peak thrombin generation by 25% but had no significant effect on PAR1-AP–mediated thrombin generation. In conclusion, stimulation of PAR4 on platelets leads to faster and more robust thrombin generation, compared with PAR1 stimulation. The greater procoagulant potential is related to more efficient FV release from intracellular stores and microparticle production driven by stronger and more sustained myosin light chain phosphorylation. These data have implications about the role of PAR4 during hemostasis and are clinically relevant in light of recent efforts to develop PAR antagonists to treat thrombotic disorders. PMID:23307185

  14. Etude du vieillissement de la glace Ih par mesures de frottement interne et par observation en topographie aux rayons X

    E-print Network

    Paris-Sud XI, Université de

    1473 Etude du vieillissement de la glace Ih par mesures de frottement interne et par observation en par mesures de frottement interne et par des observations en topographie aux rayons X. Ces mesures défauts de réseau. En effet, nos études antérieures [11, 14, 17] portant sur le frottement interne de la

  15. Ultrastructural and immunocytochemical studies of the viscacha (Lagostomus maximus maximus) pituitary pars tuberalis.

    PubMed

    Perez Romera, Edith; Mohamed, Fabian; Filippa, Verónica; Fogal, Teresa; Dominguez, Susana; Scardapane, Luis; Piezzi, Ramón

    2005-05-01

    The hypophyseal pars tuberalis (PT) has been the focus of numerous studies attempting to understand its physiological role in the reproductive regulation and modulation by the neuroendocrine system. Ultrastructural studies of the PT in a number of species have shown that it consists of a well-developed hypophyseal area with important secretory activity, demonstrated by the abundance of secretory granules in the cytoplasm and the marked blood irrigation. This article describes ultrastructural and immunocytochemical aspects of the PT in viscachas captured in their habitat. The cell types identified were PT-specific cells, agranulated cells, and Folliculostellate cells. PT-specific cells are divided into type I and II. Type I cells have cytoplasms with secretory granules of 150-500 nm diameter. The secretory granules of type II PT-specific cells are 65-200 nm in diameter. Both cellular types exhibit numerous nerve endings on the plasmatic membranes. Agranulated cells exhibit nuclei with lax chromatin, mitochondria, phagosomes, scarce Golgi complex, and rough endoplasmic reticulum. Folliculostellate cells exhibit an irregularly shaped and moderately condensed nucleus. All the described cellular types exhibit deposits of cytoplasmic glycogen. The immunocytochemical study revealed the presence of cells immunostained for LH-beta and FSH-beta in the PT caudal zone. ACTH was only detected in the zona tuberalis. No staining was observed with antiprolactin, anti-TSH-beta, and anti-GH sera. Folliculostellate cells exhibited staining with anti-S-100. The results demonstrate that the viscacha PT is a hypophyseal zone with specific cellular types, which exhibits evident secretory activity. The presence of nerve endings suggests neural control of the function of PT cells. PMID:15791582

  16. Seasonal relationships between dopamine D1 and D2 receptor and equine FSH receptor mRNA in equine ovarian epithelium.

    PubMed

    King, S S; Jones, K L; Mullenix, B A; Heath, D T

    2008-10-01

    Dopamine (DA) blockade during anestrus or early spring transition can facilitate ovarian recrudescence and advance the timing of the first ovulation of the season. Some laboratories have reported variable results using DA antagonists to stimulate follicular growth during the mid-portion of the anestrual period. Differences in DA antagonist efficacy may be due to the FSH secretory status of the anestrous mare and the presence or absence of functional ovarian FSH receptors. We hypothesize that direct ovarian dopaminergic input can affect follicular growth through regulation of FSH receptor (FSHr) populations. To investigate this, the amount of DA D1 and D2 receptor (D1r, D2r) and FSHr mRNA was quantified in ovarian tissues in anestrous and mares expressing estrus at typical intervals that are detected during the breeding season. Ovaries (n=26) were collected from 10 anestrous mares and 13 mares that had initiated estrous cycles (n=8 luteal; n=5 follicular phase). The quantity of D1r and D2r mRNA and FSHr mRNA was determined in cortex of both groups and granulosa/theca (those having initiated estrous cycles) tissues by semi-quantitative polymerase chain reaction using the comparative cycle time method. The reference gene was glyceraldehyde-3-phosphate dehydrogenase. The fold-change for each sample was calculated based on a calibrator sample. Fold-change values for D1r and D2r were the dependent variable and tissue was the independent variable in a one-way ANOVA. Results of fold-change in FSHr were compared by ANCOVA due to unequal sample sizes from each mare. Correlations between receptors within each tissue type were determined. For each receptor type and tissue, correlations between follicular and luteal phases were determined. The fold-change of D1r mRNA was less than D2r mRNA in all tissue types and between seasons. The quantity of D2r message in ovarian cortex was greater (p<0.05) during anestrus than after estrous cycles had been initiated. Fold-change in D2r in granulosa/theca was not different dependant on estrous cycle phase or follicle size. Quantity of FSHr mRNA was less in anestrous ovarian cortex and greater after estrous cycles had been initiated. FSHr mRNA fold-change in the ovarian cortex after estrous cycle initiation was not different between estrous cycle phases, but was greater in smaller (<30mm) follicles compared with larger (> or =30mm) follicles. We have demonstrated an inverse temporal relation between ovarian D2r and FSHr in mares dependant upon season. The functional significance of this relationship deserves further study. PMID:17935915

  17. Urticária e angiodema associados a fator estimulador das colônias de granulócitos e macrófagos recombinante humano (rhuGM- CSF) em portador de melanoma metastático* Urticaria and angiodema following subcutaneous injection of recombinant human granulocyte-macrophage colony- stimulating factor (rhuGM-CSF) in a patient with metastatic

    Microsoft Academic Search

    Mario Geller; Priscila Geler Wolff

    2 Resumo: É apresentado um caso de melanoma metastático controlado há seis meses com o fator estimulador das colônias de granulócitos e macrófagos recombinante humano (rhuGM- CSF), administrado periodicamente por via subcutânea. Os autores descrevem, pela primei- ra vez no Brasil, a presença de urticária gigante e angioedema associados a essa bioquimiote- rapia imunoestimulatória, tendo controlado essas reações adversas com

  18. CFIP2006-POSTPRINT Prdiction de mobilit par le mobile ou par

    E-print Network

    Paris-Sud XI, Université de

    as guessing a mobile node's next access point as it moves through a wireless network. Those predictions help garantie de qualité de service (QoS) minimale. Lorsqu'un terminal mobile se déplace, une des causesCFIP2006-POSTPRINT Prédiction de mobilité par le mobile ou par le point d'accès : comparaison sur

  19. L'ABSORPTION DES ULTRASONS PAR CERTAINES CTONES Par S. PARTHASARATHY et V. NARASIMHAN,

    E-print Network

    Paris-Sud XI, Université de

    957. L'ABSORPTION DES ULTRASONS PAR CERTAINES CÉTONES Par S. PARTHASARATHY et V. NARASIMHAN coefficient d'absorption ont été mesurés dans plusieurs cétones à différentes températures et les résultats variation de l'absorption avec la température était positif dans l'intervalle considéré dans ce travail

  20. MRI Asymmetries of Broca's Area: The Pars Triangularis and Pars Opercularis

    Microsoft Academic Search

    Anne L. Foundas; Kathy F. Eure; Laura F. Luevano; Daniel R. Weinberger

    1998-01-01

    Broca's area, which includes the pars triangularis (PTR) and pars opercularis (POP), is a neuroanatomic region important in speech-language production. Previous data demonstrated that PTR asymmetries are highly correlated with language dominance determined by selective hemispheric anesthesia or Wada testing, suggesting that asymmetries of the PTR may, in part, predict language dominance. The POP, however, has not been measured on

  1. “Platelet-associated regulatory system (PARS)” with particular reference to female reproduction

    PubMed Central

    2014-01-01

    Background Blood platelets play an essential role in hemostasis, thrombosis and coagulation of blood. Beyond these classic functions their involvement in inflammatory, neoplastic and immune processes was also investigated. It is well known, that platelets have an armament of soluble molecules, factors, mediators, chemokines, cytokines and neurotransmitters in their granules, and have multiple adhesion molecules and receptors on their surface. Methods Selected relevant literature and own views and experiences as clinical observations have been used. Results Considering that platelets are indispensable in numerous homeostatic endocrine functions, it is reasonable to suppose that a platelet-associated regulatory system (PARS) may exist; internal or external triggers and/or stimuli may complement and connect regulatory pathways aimed towards target tissues and/or cells. The signal (PAF, or other tissue/cell specific factors) comes from the stimulated (by the e.g., hypophyseal hormones, bacteria, external factors, etc.) organs or cells, and activates platelets. Platelet activation means their aggregation, sludge formation, furthermore the release of the for-mentioned biologically very powerful factors, which can locally amplify and deepen the tissue specific cell reactions. If this process is impaired or inhibited for any reason, the specifically stimulated organ shows hypofunction. When PARS is upregulated, organ hyperfunction may occur that culminate in severe diseases. Conclusion Based on clinical and experimental evidences we propose that platelets modulate the function of hypothalamo-hypophyseal-ovarian system. Specifically, hypothalamic GnRH releases FSH from the anterior pituitary, which induces and stimulates follicular and oocyte maturation and steroid hormone secretion in the ovary. At the same time follicular cells enhance PAF production. Through these pathways activated platelets are accumulated in the follicular vessels surrounding the follicle and due to its released soluble molecules (factors, mediators, chemokines, cytokines, neurotransmitters) locally increase oocyte maturation and hormone secretion. Therefore we suggest that platelets are not only a small participant but may be the conductor or active mediator of this complex regulatory system which has several unrevealed mechanisms. In other words platelets are corpuscular messengers, or are more than a member of the family providing hemostasis. PMID:24883111

  2. T(3) rapidly regulates several steps of alpha subunit glycoprotein (CGA) synthesis and secretion in the pituitary of male rats: Potential repercussions on TSH, FSH and LH secretion.

    PubMed

    Bargi-Souza, Paula; Romano, Renata Marino; Goulart-Silva, Francemilson; Brunetto, Erika Lia; Nunes, Maria Tereza

    2015-07-01

    TSH, FSH and LH share the same glycoprotein alpha chain (CGA) as part of their protein structure. Therefore, it is possible that thyroid and gonadal dysfunction may affect the CGA expression. This study evaluated several steps of CGA synthesis and secretion in thyrotrophs and gonadotrophs of control and hypothyroid rats, acutely or chronically-treated with T3. Hypothyroidism increased the Cga mRNA expression and its association to ribosome, but decreased intracellular CGA content. These parameters were reversed after acute or chronic T3 treatment. We conclude that T3 not only down-regulates Cga mRNA expression, as expected, but also inhibits the association of Cga mRNA to ribosome, as well as the CGA secretion. These findings add novel insights into our understanding of the role of T3 on the regulation of the Cga gene expression and CGA secretion, which might have a potential repercussion in all pituitary glycoprotein hormone synthesis and secretion. PMID:25869399

  3. Is assessment of anti-Müllerian hormone and/or antral follicle count useful in the prediction of ovarian response in expected normal responders treated with a fixed dose of recombinant FSH and GnRH antagonists? A prospective observational study.

    PubMed

    Ganidou, Maria A; Kolibianakis, Efstratios M; Venetis, Christos A; Gerou, Spiros; Makedos, Georgios A; Klearchou, Nikolaos; Tarlatzis, Basil C

    2014-11-01

    The purpose of this prospective observational study was to evaluate whether the assessment of AMH and AFC is useful in the prediction of ovarian response in expected normal responders treated with a fixed dose of recombinant FSH (rec-FSH) and GnRH antagonists. A base model including age and basal FSH as independent predictors of COCs could explain 15% of the variance observed in the number of COCs retrieved (p?=?0.002). The addition of AFC did not increase significantly the predictive ability of the above model, whereas the addition of AMH increased the performance of the base model by 13% (p?FSH, its predictive capacity for high ovarian response was statistically significant (F-test: p?=?0.001; c-statistic: 0.80, 95% CI: 0.70-0.88), but this was not the case for poor ovarian response. In conclusion, the addition of AMH, but not of AFC, to a model including female age and basal FSH, is useful in the prediction of ovarian response in expected normal responders treated with a fixed dose of recombinant FSH and GnRH antagonists. PMID:24989633

  4. Free Fatty Acids Induce Lhb mRNA but Suppress Fshb mRNA in Pituitary L?T2 Gonadotropes and Diet-Induced Obesity Reduces FSH Levels in Male Mice and Disrupts the Proestrous LH/FSH Surge in Female Mice

    PubMed Central

    Sharma, Shweta; Morinaga, Hidetaka; Hwang, Vicky; Fan, WuQiang; Fernandez, Marina O.; Varki, Nissi; Olefsky, Jerrold M.

    2013-01-01

    Female obesity is associated with insulin resistance, hyperandrogenemia, and reproductive dysfunction. We hypothesized that elevated free fatty acids (FFAs) might directly modulate pituitary gonadotropin production. FFAs caused a time- and dose-dependent increase in phosphorylation of the MAPKs p38MAPK, c-Jun N-terminal kinase (JNK)-1/2, and ERK1/2 in L?T2 gonadotrope cells. Furthermore, FFAs up-regulated Lhb mRNA expression acutely, an effect that was blocked by JNK inhibition, but suppressed Fshb mRNA expression, an effect that was independent of MAPK signaling. FFAs enhanced the activation of the MAPKs in the presence of GnRH, although the cotreatment did not alter Lhb induction but did eliminate the GnRH induction of Fshb. FFAs also suppressed activin-induced Fshb expression. Knockdown experiments showed that the FFA effect on the inflammatory kinases p38MAPK and JNK and on Lhb, but not Fshb, mRNA expression is mediated via toll-like receptor-2 and toll-like receptor-4 and was mimicked by lipopolysaccharide stimulation. In vivo, male C57BL/6 mice on a high-fat diet showed reduced FSH levels consistent with the suppression of Fshb seen in vitro. Histological analysis of the testes showed an increased number of abnormal seminiferous tubules. Female mice on a high-fat diet lacked the expected proestrus LH and FSH surge and exhibited an increase in the number of days at estrus and a reduced number of days at proestrus, and ovaries had significantly fewer corpora lutea. Taken together, our findings suggest that lipid excess can lead to reproductive defects in both male and female mice. PMID:23525221

  5. Kallikrein 6 Signals through PAR1 and PAR2 to Promote Neuron Injury and Exacerbate Glutamate Neurotoxicity

    PubMed Central

    Yoon, Hyesook; Radulovic, Maja; Wu, Jianmin; Blaber, Sachiko I.; Blaber, Michael; Fehlings, Michael G.; Scarisbrick, Isobel A.

    2014-01-01

    CNS trauma generates a proteolytic imbalance contributing to secondary injury, including axonopathy and neuron degeneration. Kallikrein 6 (Klk6) is a serine protease implicated in neurodegeneration and here we investigate the role of protease activated receptors 1 (PAR1) and PAR2 in mediating these effects. First we demonstrate Klk6 and the prototypical activator of PAR1, thrombin, as well as PAR1 and PAR2, are each elevated in murine experimental traumatic spinal cord injury (SCI) at acute or subacute time points. Recombinant Klk6 triggered ERK1/2 signaling in cerebellar granule neurons and in the NSC34 spinal cord motoneuron cell line, in a PI3K and MEK-dependent fashion. Importantly, lipopeptide inhibitors of PAR1 or PAR2, and PAR1 genetic deletion, each reduced Klk6-ERK1/2 activation. In addition, Klk6 and thrombin promoted degeneration of cerebellar neurons and exacerbated glutamate neurotoxicity. Moreover, genetic deletion of PAR1 blocked thrombin-mediated cerebellar neurotoxicity and reduced the neurotoxic effects of Klk6. Klk6 also increased glutamate-mediated Bim signaling, PARP cleavage and lactate dehydrogenase (LDH) release in NSC34 motoneurons and these effects were blocked by PAR1 and PAR2 lipopeptide inhibitors. Taken together these data point to a novel Klk6-signaling axis in CNS neurons that is mediated by PAR1 and PAR2 and is positioned to contribute to neurodegeneration. PMID:23647384

  6. Parasitology http://journals.cambridge.org/PAR

    E-print Network

    Schnaufer, Achim

    Parasitology http://journals.cambridge.org/PAR Additional services for Parasitology: Email alerts Parasitology / Volume 139 / Special Issue 09 / August 2012, pp 1168 1177 DOI: 10.1017/S0031182012000443 brucei cytoskeleton: advances and opportunities. Parasitology, 139, pp 11681177 doi:10.1017/S

  7. Parasitology http://journals.cambridge.org/PAR

    E-print Network

    Paris-Sud XI, Université de

    Parasitology http://journals.cambridge.org/PAR Additional services for Parasitology: Email alerts. BELTRAN, R. POULIN and C. LAGRUE Parasitology / FirstView Article / October 2012, pp 1 9 DOI: 10.1017/S parvum (Trematoda) life history strategy, size and egg production. Parasitology, Available on CJO doi:10

  8. Prsent par Leonardo-Geo MANESCU

    E-print Network

    Paris-Sud XI, Université de

    THÈSE Présenté par Leonardo-Geo MANESCU Ingénieur de l'Université de Craiova / ROUMANIE Pour obtenir le titre de DOCTEUR de l'INSTITUT NATIONAL POLYTECHNIQUE DE GRENOBLE et de l'UNIVERSITÉ DE CRAIOVA Laboratoire d'Électotechnique de Grenoble et de la Faculté d'Électrotechnique de l'Université de Craiova tel

  9. 235 -2010 Anne : 2010 Dlivre par

    E-print Network

    Sandrine ISOARD-GAUTHEUR LE BURNOUT SPORTIF CHEZ DES ADOLESCENTS(TES) EN POLE ESPOIR HANDBALL : APPROCHE tel-00842944,version1-9Jul2013 #12;Le Burnout Sportif chez des Adolescents(tes) en Pôle Espoir premier chapitre présente une revue de littérature sur le burnout sportif. Par la suite trois axes de

  10. Intramural Sports Par 3 Golf League

    E-print Network

    Escher, Christine

    Intramural Sports Par 3 Golf League Summer 2014 Intramural Sports Calendar of Events Summer 2014 Sports and Special Programs Office 111 Dixon Recreation Center (541)737-4083 Summer Office Hours: 1:00pm to 4:00pm M-F http://oregonstate.edu/recsports/intramural-sports Oregon State University

  11. suPAR and Team Nephrology

    PubMed Central

    2014-01-01

    Primary focal segmental glomerulosclerosis (FSGS) accounts for nearly 10 % of patients who require renal replacement therapy. Elevated circulating levels of soluble urokinase receptor (suPAR) have been identified as a biomarker to discriminate primary FSGS from other glomerulopathies. Subsequent reports have questioned the diagnostic utility of this test. In a study in BMC Medicine, Huang et al. demonstrate that urinary soluble urokinase receptor (suPAR) excretion assists in distinguishing primary FSGS from other glomerular diseases, and that high plasma suPAR concentrations are not directly linked to a decline in glomerular filtration rate (GFR). This observation suggests that further investigation of suPAR is warranted in patients with FSGS. It should be interpreted in light of a recent report that B7-1 is expressed in the podocytes of a subset of patients with FSGS, and that blocking this molecule may represent the first successful targeted intervention for this disease. These advances highlight the rapid pace of scientific progress in the field of nephrology. Nephrologists should work together, share resources, and expedite the design of protocols to evaluate these novel biomarkers in a comprehensive and scientifically valid manner. Please see related article http://www.biomedcentral.com/1741-7015/12/81. PMID:24885021

  12. Recherche dirigée par le dernier conflit

    Microsoft Academic Search

    Christophe Lecoutre; Lakhdar Sais; Sébastien Tabary; Vincent Vidal

    2006-01-01

    In this paper, we propose an approach to guide search to sources of conflicts. The principle is the fol- lowing: the last variable involved in the last conflict is selected in priority, as long as the constraint net- work can not be made consistent, in order to find the (most recent) culprit variable, following the current par- tial instantiation from

  13. Prsente par Pour obtenir le grade de

    E-print Network

    Paris-Sud XI, Université de

    Présentée par !" # $ Pour obtenir le grade de %&'"()"*& + , Apprentissage autonome de réseaux de neurones pour le pilotage en temps réel des systèmes de production basé sur l'optimisation via simulation, auront contribué à ce grand effort car, si l'épreuve est individuelle, ses implications sont sociales

  14. INFLUENCE DE L'IMMUNIT PASSIVE TRANSMISE PAR LE COLOSTRUM

    E-print Network

    Paris-Sud XI, Université de

    INFLUENCE DE L'IMMUNITÉ PASSIVE TRANSMISE PAR LE COLOSTRUM SUR L'ÉVOLUTION DE LA PESTE PORCINE transmise par le colostrum a permis de mettre en évidence chez r8 porcelets, de 20 à 25 kg, issus de truies transmise par le colostrum. Les applications qu'entraînent ces observations font l'objet de la discussion

  15. Rdig par Imane Hammouali Anne Scolaire 2009/2010 1

    E-print Network

    Sart, Remi

    Rédigé par Imane Hammouali Année Scolaire 2009/2010 1 Laboratoire de Météorologie Physique (La-parallèle homogène Réalisé par : Imane Hammouali Tuteurs de stage: Mr. Frédéric Szczap (LaMP) -Mr. Thierry Faure 31 Juillet 2010 #12;Rédigé par Imane Hammouali Année Scolaire 2009/2010 2 Remerciements Je tiens à

  16. Asservissement par PID Application du PWM au pont en H

    E-print Network

    Tardieu, Samuel

    Plan Asservissement par PID PWM Ponts en H Application du PWM au pont en H Bibliographie Asservissement par PID, PWM et Ponts en H Siwar, Cédric, Samuel Télécom Paristech 4 mars 2011 Siwar, Cédric, Samuel ROSE 1 / 33 4 mars 2011 #12;Plan Asservissement par PID PWM Ponts en H Application du PWM au pont

  17. Contrle de drones et robots par reconnaissance de mouvements complexes

    E-print Network

    Boyer, Edmond

    Contrôle de drones et robots par reconnaissance de mouvements complexes Anaïs Finzi, Bastien Tauran contexte du pilotage de drones et de robots par des mouvements de l'opérateur détectés par des applied to complex gesture recognition in the context of drones and robots control, when the human

  18. Amlioration de l'utilisation par les bovins des chaumes de Sorghum bicolor par le hachage et la complmentation

    E-print Network

    Boyer, Edmond

    Amélioration de l'utilisation par les bovins des chaumes de Sorghum bicolor par le hachage et la and Leng, 1986 ILCA/Addis Ababa). Un essai d'amélioration de pailles de Sorghum bicolor complémenté à l

  19. Macrophage Migration Inhibitory Factor Mediates PAR-Induced Bladder Pain

    PubMed Central

    Kouzoukas, Dimitrios E.; Meyer-Siegler, Katherine L.; Ma, Fei; Westlund, Karin N.; Hunt, David E.; Vera, Pedro L.

    2015-01-01

    Introduction Macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine, is constitutively expressed in urothelial cells that also express protease-activated receptors (PAR). Urothelial PAR1 receptors were shown to mediate bladder inflammation. We showed that PAR1 and PAR4 activator, thrombin, also mediates urothelial MIF release. We hypothesized that stimulation of urothelial PAR1 or PAR4 receptors elicits release of urothelial MIF that acts on MIF receptors in the urothelium to mediate bladder inflammation and pain. Thus, we examined the effect of activation of specific bladder PAR receptors on MIF release, bladder pain, micturition and histological changes. Methods MIF release was measured in vitro after exposing immortalized human urothelial cells (UROtsa) to PAR1 or PAR4 activating peptides (AP). Female C57BL/6 mice received intravesical PAR1- or PAR4-AP for one hour to determine: 1) bladder MIF release in vivo within one hour; 2) abdominal hypersensitivity (allodynia) to von Frey filament stimulation 24 hours after treatment; 3) micturition parameters 24 hours after treatment; 4) histological changes in the bladder as a result of treatment; 5) changes in expression of bladder MIF and MIF receptors using real-time RT-PCR; 6) changes in urothelial MIF and MIF receptor, CXCR4, protein levels using quantitative immunofluorescence; 7) effect of MIF or CXCR4 antagonism. Results PAR1- or PAR4-AP triggered MIF release from both human urothelial cells in vitro and mouse urothelium in vivo. Twenty-four hours after intravesical PAR1- or PAR4-AP, we observed abdominal hypersensitivity in mice without changes in micturition or bladder histology. PAR4-AP was more effective and also increased expression of bladder MIF and urothelium MIF receptor, CXCR4. Bladder CXCR4 localized to the urothelium. Antagonizing MIF with ISO-1 eliminated PAR4- and reduced PAR1-induced hypersensitivity, while antagonizing CXCR4 with AMD3100 only partially prevented PAR4-induced hypersensitivity. Conclusions Bladder PAR activation elicits urothelial MIF release and urothelial MIF receptor signaling at least partly through CXCR4 to result in abdominal hypersensitivity without overt bladder inflammation. PAR-induced bladder pain may represent an interesting pre-clinical model of Interstitial Cystitis/Painful Bladder Syndrome (IC/PBS) where pain occurs without apparent bladder injury or pathology. MIF is potentially a novel therapeutic target for bladder pain in IC/PBS patients. PMID:26020638

  20. Phosphorylation of Mycobacterium tuberculosis ParB Participates in Regulating the ParABS Chromosome Segregation System

    PubMed Central

    Baronian, Grégory; Ginda, Katarzyna; Berry, Laurence; Cohen-Gonsaud, Martin; Zakrzewska-Czerwi?ska, Jolanta; Jakimowicz, Dagmara; Molle, Virginie

    2015-01-01

    Here, we present for the first time that Mycobacterium tuberculosis ParB is phosphorylated by several mycobacterial Ser/Thr protein kinases in vitro. ParB and ParA are the key components of bacterial chromosome segregation apparatus. ParB is a cytosolic conserved protein that binds specifically to centromere-like DNA parS sequences and interacts with ParA, a weak ATPase required for its proper localization. Mass spectrometry identified the presence of ten phosphate groups, thus indicating that ParB is phosphorylated on eight threonines, Thr32, Thr41, Thr53, Thr110, Thr195, and Thr254, Thr300, Thr303 as well as on two serines, Ser5 and Ser239. The phosphorylation sites were further substituted either by alanine to prevent phosphorylation or aspartate to mimic constitutive phosphorylation. Electrophoretic mobility shift assays revealed a drastic inhibition of DNA-binding by ParB phosphomimetic mutant compared to wild type. In addition, bacterial two-hybrid experiments showed a loss of ParA-ParB interaction with the phosphomimetic mutant, indicating that phosphorylation is regulating the recruitment of the partitioning complex. Moreover, fluorescence microscopy experiments performed in the surrogate Mycobacterium smegmatis ?parB strain revealed that in contrast to wild type Mtb ParB, which formed subpolar foci similar to M. smegmatis ParB, phoshomimetic Mtb ParB was delocalized. Thus, our findings highlight a novel regulatory role of the different isoforms of ParB representing a molecular switch in localization and functioning of partitioning protein in Mycobacterium tuberculosis. PMID:25807382

  1. THORIE DE LA CAPTURE SLECTIVE DES NEUTRONS LENTS PAR CERTAINS NOYAUX Par FRANCIS PETRIN et WALTER M. ELSASSER.

    E-print Network

    Boyer, Edmond

    THÉORIE DE LA CAPTURE SÉLECTIVE DES NEUTRONS LENTS PAR CERTAINS NOYAUX Par FRANCIS PETRIN et WALTER neutrons lents observées pour quelques éléments par Fermi (plus de mille fois la section nucléaire), sans supposer d'action à distance entre les neutrons et les noyaux (1). Pour une interaction limitée au domaine

  2. Similar requirements for CDC-42 and the PAR-3/PAR-6/PKC-3 complex in diverse cell types

    E-print Network

    Ahringe, Julie

    Similar requirements for CDC-42 and the PAR-3/PAR-6/PKC-3 complex in diverse cell types David P kinase C (aPKC) plays a central role in cell polarisation. The small G protein Cdc42 also functions, it is not yet known whether Cdc42 and the Par3 complex widely function together in development or whether

  3. Critical Role for PAR1 in Kallikrein 6-Mediated Oligodendrogliopathy

    PubMed Central

    Burda, Joshua E.; Radulovic, Maja; Yoon, Hyesook; Scarisbrick, Isobel A.

    2014-01-01

    Kallikrein 6 (Klk6) is a secreted serine protease preferentially expressed by oligodendroglia in CNS white matter. Elevated levels of Klk6 occur in actively demyelinating multiple sclerosis (MS) lesions and in cases of spinal cord injury (SCI), stroke and glioblastoma. Taken with recent evidence establishing Klk6 as a CNS-endogenous activator of protease-activated receptors (PARs), we hypothesized that Klk6 activates a subset of PARs to regulate oligodendrocyte physiology and potentially pathophysiology. Here, primary oligodendrocyte cultures derived from wild type or PAR1-deficient mice and the murine oligodendrocyte cell line, Oli-neu, were used to demonstrate that Klk6 mediates loss of oligodendrocyte processes and impedes morphological differentiation of oligodendrocyte progenitor cells (OPCs) in a PAR1-dependent fashion. Comparable gliopathy was also elicited by the canonical PAR1 agonist, thrombin, as well as PAR1-activating peptides (PAR1-APs). Klk6 also exacerbated ATP-mediated oligodendrogliopathy in vitro, pointing to a potential role in augmenting excitotoxicity. In addition, Klk6 suppressed the expression of proteolipid protein (PLP) RNA in cultured oligodendrocytes by a mechanism involving PAR1-mediated Erk1/2 signaling. Microinjection of PAR1 agonists, including Klk6 or PAR1-APs, into the dorsal column white matter of PAR+/+ but not PAR?/? mice promoted vacuolating myelopathy and a loss of immunoreactivity for myelin basic protein (MBP) and CC-1+ oligodendrocytes. These results demonstrate a functional role for Klk6-PAR1 signaling in oligodendroglial pathophysiology and suggest that PAR1 or PAR1-agonists may represent new targets to moderate demyelination and to promote myelin regeneration in cases of CNS white matter injury or disease. PMID:23832758

  4. A Single parS Sequence from the Cluster of Four Sites Closest to oriC Is Necessary and Sufficient for Proper Chromosome Segregation in Pseudomonas aeruginosa

    PubMed Central

    Jecz, Paulina; Bartosik, Aneta A.; Glabski, Krzysztof; Jagura-Burdzy, Grazyna

    2015-01-01

    Among the mechanisms that control chromosome segregation in bacteria are highly-conserved partitioning systems comprising three components: ParA protein (a deviant Walker-type ATPase), ParB protein (a DNA-binding element) and multiple cis-acting palindromic centromere-like sequences, designated parS. Ten putative parS sites have been identified in the P. aeruginosa PAO1 genome, four localized in close proximity of oriC and six, diverged by more than one nucleotide from a perfect palindromic sequence, dispersed along the chromosome. Here, we constructed and analyzed P. aeruginosa mutants deprived of each single parS sequence and their different combinations. The analysis included evaluation of a set of phenotypic features, chromosome segregation, and ParB localization in the cells. It was found that ParB binds specifically to all ten parS sites, although with different affinities. The P. aeruginosa parS mutant with all ten parS sites modified (parSnull) is viable however it demonstrates the phenotype characteristic for parAnull or parBnull mutants: slightly slower growth rate, high frequency of anucleate cells, and defects in motility. The genomic position and sequence of parS determine its role in P. aeruginosa biology. It transpired that any one of the four parS sites proximal to oriC (parS1 to parS4), which are bound by ParB with the highest affinity, is necessary and sufficient for the parABS role in chromosome partitioning. When all these four sites are mutated simultaneously, the strain shows the parSnull phenotype, which indicates that none of the remaining six parS sites can substitute for these four oriC-proximal sites in this function. A single ectopic parS2 (inserted opposite oriC in the parSnull mutant) facilitates ParB organization into regularly spaced condensed foci and reverses some of the mutant phenotypes but is not sufficient for accurate chromosome segregation. PMID:25794281

  5. The PAR Proteins: Fundamental Players in Animal Cell Polarization

    PubMed Central

    Goldstein, Bob; Macara, Ian G.

    2010-01-01

    The par genes were discovered in genetic screens for regulators of cytoplasmic partitioning in the early embryo of C. elegans, and encode six different proteins required for asymmetric cell division by the worm zygote. Some of the PAR proteins are localized asymmetrically and form physical complexes with one another. Strikingly, the PAR proteins have been found to regulate cell polarization in many different contexts in diverse animals, suggesting they form part of an ancient and fundamental mechanism for cell polarization. Although the picture of how the PAR proteins function remains incomplete, cell biology and biochemistry are beginning to explain how PAR proteins polarize cells. PMID:17981131

  6. A polymorphic protease-activated receptor 2 (PAR2) displaying reduced sensitivity to trypsin and differential responses to PAR agonists.

    PubMed

    Compton, S J; Cairns, J A; Palmer, K J; Al-Ani, B; Hollenberg, M D; Walls, A F

    2000-12-15

    Protease-activated receptor 2 (PAR2) is a trypsin-activated member of a family of G-protein-coupled PARs. We have identified a polymorphic form of human PAR2 (PAR(2)F240S) characterized by a phenylalanine to serine mutation at residue 240 within extracellular loop 2, with allelic frequencies of 0.916 (Phe(240)) and 0.084 (Ser(240)) for the wild-type and mutant alleles, respectively. Elevations in intracellular calcium were measured in permanently transfected cell lines expressing the receptors. PAR(2)F240S displayed a significant reduction in sensitivity toward trypsin ( approximately 3.7-fold) and the PAR2-activating peptides, SLIGKV-NH(2) ( approximately 2.5-fold) and SLIGRL-NH(2) ( approximately 2.8-fold), but an increased sensitivity toward the selective PAR2 agonist, trans-cinnamoyl-LIGRLO-NH(2) ( approximately 4-fold). Increased sensitivity was also observed toward the selective PAR-1 agonist, TFLLR-NH(2) ( approximately 7-fold), but not to other PAR-1 agonists tested. Furthermore, we found that TLIGRL-NH(2) and a PAR4-derived peptide, trans-cinnamoyl-YPGKF-NH(2), were selective PAR(2)F240S agonists. By introducing the F240S mutation into rat PAR2, we observed shifts in agonist potencies that mirrored the human PAR(2)F240S, suggesting that Phe(240) is involved in determining agonist specificity of PAR2. Finally, differences in receptor signaling were paralleled in a cell growth assay. We suggest that the distinct pharmacological profile induced by this polymorphism will have important implications for the design of PAR-targeted agonists/antagonists and may contribute to, or be predictive of, an inflammatory disease. PMID:10995771

  7. Critical role for PAR1 in kallikrein 6-mediated oligodendrogliopathy.

    PubMed

    Burda, Joshua E; Radulovic, Maja; Yoon, Hyesook; Scarisbrick, Isobel A

    2013-09-01

    Kallikrein 6 (KLK6) is a secreted serine protease preferentially expressed by oligodendroglia in CNS white matter. Elevated levels of KLK6 occur in actively demyelinating multiple sclerosis (MS) lesions and in cases of spinal cord injury (SCI), stroke, and glioblastoma. Taken with recent evidence establishing KLK6 as a CNS-endogenous activator of protease-activated receptors (PARs), we hypothesized that KLK6 activates a subset of PARs to regulate oligodendrocyte physiology and potentially pathophysiology. Here, primary oligodendrocyte cultures derived from wild type or PAR1-deficient mice and the murine oligodendrocyte cell line, Oli-neu, were used to demonstrate that Klk6 (rodent form) mediates loss of oligodendrocyte processes and impedes morphological differentiation of oligodendrocyte progenitor cells (OPCs) in a PAR1-dependent fashion. Comparable gliopathy was also elicited by the canonical PAR1 agonist, thrombin, as well as PAR1-activating peptides (PAR1-APs). Klk6 also exacerbated ATP-mediated oligodendrogliopathy in vitro, pointing to a potential role in augmenting excitotoxicity. In addition, Klk6 suppressed the expression of proteolipid protein (PLP) RNA in cultured oligodendrocytes by a mechanism involving PAR1-mediated Erk1/2 signaling. Microinjection of PAR1 agonists, including Klk6 or PAR1-APs, into the dorsal column white matter of PAR1(+/+) but not PAR1(-/-) mice promoted vacuolating myelopathy and a loss of immunoreactivity for myelin basic protein (MBP) and CC-1(+) oligodendrocytes. These results demonstrate a functional role for Klk6-PAR1 signaling in oligodendroglial pathophysiology and suggest that antagonists of PAR1 or its protease agonists may represent new modalities to moderate demyelination and to promote myelin regeneration in cases of CNS white matter injury or disease. PMID:23832758

  8. Les fréquences décrites par Paul Nogier

    Microsoft Academic Search

    Y. Rouxeville

    \\u000a Au début des années soixante-dix, nous utilisions l’EMS® avec la gamme des fréquences initiales décrites par Paul Nogier.\\u000a Ce sont des harmoniques : 2,5 Hz (A), 5 Hz (B), 10 Hz (C), 20 Hz (D), 40 Hz (E), 80 Hz (F), et 160 Hz (G). Tous les utilisateurs\\u000a en étaient fort satisfaits.

  9. Series chronologiques Prevision par lissage exponentiel

    E-print Network

    Lagnoux Renaudie, Agnès

    S´eries chronologiques - Pr´evision par lissage exponentiel Agn`es LAGNOUX lagnoux@univ-tlse2.fr pr´evision qui accordent plus ou moins d'importance aux valeurs du pass´e d'une s´erie temporelle + St + t. t Z, Xt = ZtSt + t. avec Zt une s´erie constante ou lin´eaire. #12;Le lissage exponentiel

  10. Microscopie en champ proche par réflexion

    NASA Astrophysics Data System (ADS)

    Spajer, M.; Courjon, D.; Sarayeddine, K.; Jalocha, A.; Vigoureux, J.-M.

    1991-01-01

    This communication presents two techniques for high resolution surface analysis. In the first one, the illumination beam is totally reflected under the object surface, which must be transparent. The evanescent wave confined on the surface is frustrated locally by a dielectric probe of nanometric dimension which scans the objetc. A horizontal resolution of 10 nm and a vertical one of 1 nm have been obtained. In the second one, more adapted to metallic objects, the surface is illuminated by the near-field of the stylus, in which the reflected beam is Partially launched. First results dealing with the characterization of the dielectric stylus are presented. Cette communication présente deux techniques permettant l'analyse de surface haute résolution. Dans la première, le faisceau d'éclairage est réfléchi totalement sous la surface de l'objet, nécessairement transparent. L'onde évanescente qui l'accompagne est frustrée localement par une pointe diélectrique de dimension nanométrique balayant la surface. Une résolution de 10 nm horizontalement et de 1nm verticalement a été obtenue. Dans la seconde, qui permet d'étudier un objet opaque, celui-ci est éclairé par le champ proche émis par la pointe, dans laquelle le faisceau réfléchi est partiellement réinjecté. Les premiers résultats concernant la caractérisation de la pointe sont présentés.

  11. Gene expression of ACTH, glucocorticoid receptors, 11?HSD enzymes, LH-, FSH-, GH receptors and aromatase in equine epididymal and testicular tissue.

    PubMed

    Herrera-Luna, C V; Budik, S; Aurich, C

    2012-12-01

    Glucocorticoids (GCs) are important mediators of the stress response and have been implicated in the function and regulation of testicular functions in different species. In many tissues, intracellular glucocorticoid activity is controlled by either or both of the two known isoforms of 11?-hydroxysteroid dehydrogenase (11?HSD) type 1 and 2, which interconvert active and inactive GCs. Little is known about the effects of stress on fertility in the equine species. The main objective of the present study was to investigate the expression of receptors for GCs and adrenocorticotropic hormone [ACTH, melanocortin 2 receptor (MC2R)] as well 11?HSD1 and 11?HSD2 in male equine epididymal and testicular tissue. In addition, expression of aromatase P-450 and receptors for luteinizing hormone (LHR), follicle stimulating hormone (FSHR) and growth hormone (GHR) was studied. Reverse transcriptase PCR and quantitative real-time PCR were performed in tissue from the epididymis (caput and cauda) and testes collected from nine healthy mature stallions (age 4-10 years). mRNA for ACTH and GC receptors as well as 11?HSD1 and -2 were found in epididymal and testicular tissue. Expression of the genes studied was always positive in testicular tissue, while it was inconsistent in epididymal tissue. Quantitative gene expression in relation to ?-actin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was significantly correlated (R = 0.403, p < 0.001). Quantitative PCR in relation to ?-actin revealed significant differences in the gene expression of 11?HSD1, 11?HSD2, LHR, FSHR, MC2R and aromatase between tissue collected from caput epididymidis, cauda epididymidis and testicular parenchyma (p < 0.05). With GAPDH, differences between tissues were significant for 11?HSD1, 11?HSD2 and MC2R (p < 0.05) In addition, high concentrations of mRNA of aromatase and receptors of LH and FSH were found in testicular tissue, while a pronounced expression of GH receptor was present in epididymal tissue. The results support the hypothesis of an interaction between the pituitary-adrenal axis and testicular function in the stallion. PMID:22335522

  12. Review of PAR parameterizations in ocean ecosystem models

    NASA Astrophysics Data System (ADS)

    Byun, Do-Seong; Wang, Xiao Hua; Hart, Deirdre E.; Zavatarelli, Marco

    2014-12-01

    Commonly-used empirical equations for calculating downward 'photosynthetically available radiation' or PAR were reviewed in order to identify a more theoretically-sound parameterization for application to ocean biogeochemical models. Three different forms of broadband PAR parameterization are currently employed in biogeochemical models, each of them originating from the downward irradiance formulations normally applied to ocean circulation models, which produce poor attenuation estimates for PAR. Two of the PAR formulations, a single-exponential function and a double-exponential function, are parameterized by multiplying surface irradiance by a coefficient determining the portion of underwater PAR. The third formulation uses the second term of the double-exponential function. After elucidating the theoretical problems of modeling PAR using these parameterizations, we suggest an improved, R-modified double-exponential PAR formulation, including Paulson and Simpson's (1977) parameter values. We also newly estimate PAR penetration via least-squares fitting of values digitized from Jerlov's (1976) observations in different oceanic water types, and compare this PAR-observation derived parameterization with our new, theoretical, R-modified parameterization. Finally, we discuss a universal limitation inherent in current theoretical approaches to PAR parameterization.

  13. Molecular cloning and characterization of gonadotropin subunits (GTH?, FSH? and LH?) and their regulation by hCG and GnRHa in Japanese sea bass (Lateolabrax japonicas) in vivo.

    PubMed

    Chi, Mei L; Ni, Meng; Li, Ji F; He, Feng; Qian, Kun; Zhang, Pei; Chai, Sen H; Wen, Hai S

    2015-06-01

    In this study, three cDNA sequences encoding common glycoprotein ? subunit (GTH?), follicle-stimulating hormone ? subunit (FSH?) and luteinizing hormone ? subunit (LH?) were isolated from Japanese sea bass (Lateolabrax japonicas). Comparison of the deduced amino acid sequences with other gonadotropic hormones (GTHs) indicated that their cysteine residues and potential N-linked glycosylation sites were highly conserved, and high homology with those of other perciformes was showed in phylogenetic analysis. GTHs transcripts were present highly in the pituitary and brain and weakly in testis and other tissues. During testicular development, GTHs transcriptional levels in pituitary and brain (expect FSH? subunit in brain) were significantly increased at spermiation period, stage V. Subsequently, the effects of hCG and GnRHa on the mRNA levels of GTHs subunits were examined. In brain, both hormones were detected to improve the expression of GTH? subunit mRNA. In pituitary, three GTHs subunits increased parallelly and abruptly in two hormone treatment groups. In testis, hCG was suggested to improve three GTHs subunits expression in Japanese sea bass for the first time. These results suggest that both gonadotropins are probably involved in the control of Japanese sea bass spermatogenesis and provide a framework for better understanding of the mechanisms of hormone-mediated reproduction control in Japanese sea bass and other teleosts. PMID:25724868

  14. A new method for PAR reduction in baseband DMT systems

    Microsoft Academic Search

    Brian S. Krongold; Douglas L. Jones

    2001-01-01

    The high peak-to-average power ratio (PAR) in real-baseband discrete multitone modulation (DMT) systems can significantly limit performance. Tone reservation techniques use unavailable or reserved tones to design a peak-cancelling signal that lowers the PAR of a transmit data block. Previous methods converge very slowly to a good solution. Our new active-set method converges much faster toward a minimum-PAR solution at

  15. PRODUCTION DES GRANDES VITESSES ANGULAIRES (1); Par M. MAURICE LEBLANC.

    E-print Network

    Paris-Sud XI, Université de

    redresser l'arbre flexible et à ramener son axe neutre en coïncidence avec l'axe 00'. Supposons d'abord le rotor parfaitement équilibré, par rapport à son axe de figure uv, qui sera alors son axe naturel de rotation et se confondra avec l'axe x~ de la figure 7. Désignons par 1 le moment d'inertie du rotor, par

  16. Mechanism of DNA segregation in prokaryotes: Replicon pairing by parC of plasmid R1

    PubMed Central

    Jensen, Rasmus Bugge; Lurz, Rudi; Gerdes, Kenn

    1998-01-01

    Prokaryotic chromosomes and plasmids encode partitioning systems that are required for DNA segregation at cell division. The systems are thought to be functionally analogous to eukaryotic centromeres and to play a general role in DNA segregation. The parA system of plasmid R1 encodes two proteins ParM and ParR, and a cis-acting centromere-like site denoted parC. The ParR protein binds to parC in vivo and in vitro. The ParM protein is an ATPase that interacts with ParR specifically bound to parC. Using electron microscopy, we show here that parC mediates efficient pairing of plasmid molecules. The pairing requires binding of ParR to parC and is stimulated by the ParM ATPase. The ParM mediated stimulation of plasmid pairing is dependent on ATP hydrolysis by ParM. Using a ligation kinetics assay, we find that ParR stimulates ligation of parC-containing DNA fragments. The rate-of-ligation was increased by wild type ParM protein but not by mutant ParM protein deficient in the ATPase activity. Thus, two independent assays show that parC mediates pairing of plasmid molecules in vitro. These results are consistent with the proposal that replicon pairing is part of the mechanism of DNA segregation in prokaryotes. PMID:9671715

  17. Transcriptome profiling reveals links between ParS/ParR, MexEF-OprN, and quorum sensing in the regulation of adaptation and virulence in Pseudomonas aeruginosa

    PubMed Central

    2013-01-01

    Background The ParS/ParR two component regulatory system plays critical roles for multidrug resistance in Pseudomonas aeruginosa. It was demonstrated that in the presence of antimicrobials, ParR enhances bacterial survival by distinct mechanisms including activation of the mexXY efflux genes, enhancement of lipopolysaccharide modification through the arn operon, and reduction of the expression of oprD porin. Results In this study, we report on transcriptomic analyses of P. aeruginosa PAO1 wild type and parS and parR mutants growing in a defined minimal medium. Our transcriptomic analysis provides the first estimates of transcript abundance for the 5570 coding genes in P. aeruginosa PAO1. Comparative transcriptomics of P. aeruginosa PAO1 and par mutants identified a total of 464 genes regulated by ParS and ParR. Results also showed that mutations in the parS/parR system abolished expression of the mexEF-oprN operon by down-regulating the regulatory gene mexS. In addition to the known effects on drug resistance genes, transcript abundances of the quorum sensing genes (rhlIR and pqsABCDE-phnAB) were higher in both parS and parR mutants. In accordance with these results, a significant portion of the ParS/ParR regulated genes belonged to the MexEF-OprN and quorum sensing regulons. Deletion of the par genes also led to increased phenazine production and swarming motility, consistent with the up-regulation of the phenazine and rhamnolipid biosynthetic genes, respectively. Conclusion Our results link the ParS/ParR two component signal transduction system to MexEF-OprN and quorum sensing systems in P. aeruginosa. These results expand our understanding of the roles of the ParS/ParR system in the regulation of gene expression in P. aeruginosa, especially in the absence of antimicrobials. PMID:24034668

  18. ParABS System in Chromosome Partitioning in the Bacterium Myxococcus xanthus

    PubMed Central

    Iniesta, Antonio A.

    2014-01-01

    Chromosome segregation is an essential cellular function in eukaryotic and prokaryotic cells. The ParABS system is a fundamental player for a mitosis-like process in chromosome partitioning in many bacterial species. This work shows that the social bacterium Myxococcus xanthus also uses the ParABS system for chromosome segregation. Its large prokaryotic genome of 9.1 Mb contains 22 parS sequences near the origin of replication, and it is shown here that M. xanthus ParB binds preferentially to a consensus parS sequence in vitro. ParB and ParA are essential for cell viability in M. xanthus as in Caulobacter crescentus, but unlike in many other bacteria. Absence of ParB results in anucleate cells, chromosome segregation defects and loss of viability. Analysis of ParA subcellular localization shows that it clusters at the poles in all cells, and in some, in the DNA-free cell division plane between two chromosomal DNA masses. This ParA localization pattern depends on ParB but not on FtsZ. ParB inhibits the nonspecific interaction of ParA with DNA, and ParA colocalizes with chromosomal DNA only when ParB is depleted. The subcellular localization of ParB suggests a single ParB-parS complex localized at the edge of the nucleoid, next to a polar ParA cluster, with a second ParB-parS complex migrating after the replication of parS takes place to the opposite nucleoid edge, next to the other polar ParA cluster. PMID:24466283

  19. THROMBIN REGULATES METASTATIC POTENTIAL OF HUMAN RHABDOMYOSARCOMA CELLS – DISTINCT ROLE OF PAR1 AND PAR3 SIGNALING

    PubMed Central

    Wysoczynski, Marcin; Rui, Liu; Kucia, Magda; Drukala, Justyna; Ratajczak, Mariusz Z.

    2010-01-01

    We observed that human rhabdomyosarcoma (RMS) cells highly express a tissue factor (TF) that promotes thrombin formation, which indirectly and directly affects RMS progression. First, we found that thrombin activates platelets to generate microvesicles (PMVs), which transfer to RMS cells’ ?2?3 integrin and increase their adhesiveness to endothelial cells. Accordingly, RMS cells covered with PMVs showed higher metastatic potential after intravenous injection into immunodeficient mice. Furthermore, PMVs activate mitogen-activated protein kinase (MAPK)p42/44 and AKT to chemoattract RMS cells. We also found that RMS cells express functional protease-activated receptor-1 (PAR1) and PAR3 and respond to thrombin stimulation by MAPKp42/44 and MAPKp38 phosphorylation. To our surprise, thrombin did not affect RMS proliferation or survival; it inhibited RMS cells’ chemotactic and adhesive properties. However, when PAR1-specific agonist thrombin receptor-activating peptide (TRAP)6 was employed, which does not activate PAR3, selective PAR1 stimulation enhanced RMS proliferation. To learn more on the role of PAR1 and PAR3 antagonism in RMS proliferation and metastasis, we knocked down both receptors by employing a short hairpin (sh)RNA strategy. We found that while thrombin does not affect growth of PAR1?/? cells, it stimulated proliferation of PAR3?/? cells. More importantly, PAR3?/? cells, in contrast to PAR1?/? ones, formed larger tumors in immunodeficient mice. We conclude that thrombin is a novel; underappreciated modulator of RMS metastasis and that we have identified a novel role for PAR3 in thrombin signaling. PMID:20442298

  20. Historic macrophyte development in Par Pond

    SciTech Connect

    Grace, J.B.

    1985-08-01

    Aerial photographs from 1975, 1980, and 1983 were examined to evaluate the changes that have occurred in the wetland vegetation of Par Pond, a reactor-cooling reservoir. Evaluation of the aerial photographs was based on comparisons with ground-level vegetation maps made during July 1984. Comparisons of photographs from August and December of 1983 revealed the main seasonal change in the aerial coverage of wetland vegetation to be the wintertime loss of non-persistent emergent species such as Nelumbo lutea and Nymphaea odorata. Comparisons between September 1980 and August 1983 revealed that the lakeward extent of non-persistent macrophytes has increased by an average of 8.2 m, though not all sites have changed equally. For persistent macrophytes (principally Typha), the average increase in lakeward extent between December 1975 and August 1983 was 3.48 m. The extensive development of wetland vegetation in Par Pond as well as the substantial spread of vegetation over only a few years time indicates the high suitability of this habitat for the growth of wetland plants.

  1. Entérite lupique récidivante améliorée par Azathioprine

    PubMed Central

    Marzouk, Sameh; Garbaa, Saida; Cherif, Yosra; Jallouli, Moez; Bahri, Fathi; Bahloul, Zouhir

    2015-01-01

    Les manifestations gastro-intestinales observées au cours du lupus érythémateux systémique sont fréquentes et peuvent intéresser n'importe quel segment du tractus digestif. L'entérite lupique constitue l'une des manifestations responsable de douleurs abdominales. Son traitement est basé essentiellement sur les corticoïdes. Le recours aux immunosuppresseurs est réservé aux formes récidivantes ou en cas d’échec des corticoïdes. Nous rapportons une nouvelle observation d'entérite lupique récidivante améliorée par azathioprine. Il s'agissait d'une femme âgée de 30 ans chez laquelle le diagnostic du lupus a été retenu en 2004. Un an après, elle a présenté des douleurs abdominales, des vomissements et des diarrhées. Les explorations ont conclu à une entérite lupique après élimination de toute autre cause notamment infectieuse. Elle a été traitée par des corticoïdes à forte dose. Cependant à chaque tentative de dégression, elle présentait la même symptomatologie. En 2010 l'azathioprine a été associé permettant de juguler la maladie et de diminuer la corticothérapie. PMID:26113946

  2. Condensation and localization of the partitioning protein ParB on the bacterial chromosome

    PubMed Central

    Broedersz, Chase P.; Wang, Xindan; Meir, Yigal; Loparo, Joseph J.; Rudner, David Z.; Wingreen, Ned S.

    2014-01-01

    The ParABS system mediates chromosome segregation and plasmid partitioning in many bacteria. As part of the partitioning mechanism, ParB proteins form a nucleoprotein complex at parS sites. The biophysical basis underlying ParB–DNA complex formation and localization remains elusive. Specifically, it is unclear whether ParB spreads in 1D along DNA or assembles into a 3D protein–DNA complex. We show that a combination of 1D spreading bonds and a single 3D bridging bond between ParB proteins constitutes a minimal model for a condensed ParB–DNA complex. This model implies a scaling behavior for ParB-mediated silencing of parS-flanking genes, which we confirm to be satisfied by experimental data from P1 plasmids. Furthermore, this model is consistent with experiments on the effects of DNA roadblocks on ParB localization. Finally, we show experimentally that a single parS site is necessary and sufficient for ParB–DNA complex formation in vivo. Together with our model, this suggests that ParB binding to parS triggers a conformational switch in ParB that overcomes a nucleation barrier. Conceptually, the combination of spreading and bridging bonds in our model provides a surface tension ensuring the condensation of the ParB–DNA complex, with analogies to liquid-like compartments such as nucleoli in eukaryotes. PMID:24927534

  3. L'ACTION INTERCATHODIQUE DANS LE CHAMP MAGNTIQUE (1) ; Par M. GOUY.

    E-print Network

    Paris-Sud XI, Université de

    continu et fourni par une batterie d'accu- mulateurs ou par une machine électrostatique, et le champ magné .000 gauss, courant 200 microampères, boules de 8 millimètres en aluminium. (2) On peut, par exemple

  4. Article original Nombre optimal de ramets par clone

    E-print Network

    Paris-Sud XI, Université de

    Article original Nombre optimal de ramets par clone dans deux tests clonaux H Muranty, F Santi, LE 24 mai 1994 ; accepté le 13 avril 1995) Résumé — Le nombre optimal de ramets par clone dans des, de forme et de qualité du bois. Ces données provenaient d'un essai de 32 clones de merisier (Prunus

  5. Stganographie Adaptative par Oracle (ASO) Sarra Kouider2

    E-print Network

    Paris-Sud XI, Université de

    Stéganographie Adaptative par Oracle (ASO) Sarra Kouider2 Marc Chaumont1,2 William Puech2 1 continuité de ces travaux, nous proposons un algorithme de stéganographie adaptative par oracle (ASO : Adaptive Steganography by Oracle). Pour cela, nous définissons la carte de détectabi- lité en exploitant

  6. UNIVERSITE PARIS XIII Etudes de systmes atomiques par spectroscopie laser.

    E-print Network

    Paris-Sud XI, Université de

    . Les premières expériences réalisées avec un jet atomique thermique et des lasers à colorant sontUNIVERSITE PARIS XIII Etudes de systèmes atomiques par spectroscopie laser. Tests de physique fondamentale et développement d'un étalon de fréquence optique. Dossier de synthèse présenté par Mark David

  7. DE PROTECTION DE LA JEUNESSE: par Jacinthe MERCIERf

    E-print Network

    Spino, Claude

    DE PROTECTION DE LA JEUNESSE: par Jacinthe MERCIERf La procédure applicable en matière de Protection de la jeunesse soulève parfois des interrogations. Elle est régie par des dispositions qui se. Procedural matters relating to youth protection raise a number of diflculties due to the various legislative

  8. Utilisation des substrats glucoformateurs par les hpatocytes isols de mouton

    E-print Network

    Boyer, Edmond

    'activité de la pyruvate carboxylase. L'uréogenèse à partir de l'ammoniac (5 mM) est stimulée par le propiona lactate en pyruvate, soit d'une conversion très lente du pyru- vate en oxaloacétate. Le propionate est par

  9. MESURES ANGULAIRES PAR GYROMTRE LASER (*) ET PHNOMNES DE COUPLAGE

    E-print Network

    Paris-Sud XI, Université de

    résultats théoriques. Abstract. 2014 A theoretical analysis of coupling phenomena in a ring laser is given. Results of angular measurements are presented for a laser-gyro with Langmuir-Fizeau and Faraday effect737 MESURES ANGULAIRES PAR GYROMÈTRE LASER (*) ET PHÉNOMÈNES DE COUPLAGE par J. J. ROLAND et J. M

  10. EECSElectrical Engineering and Computer Sciences BERKELEY PAR LAB

    E-print Network

    California at Berkeley, University of

    -tuning is an automated, general system for performance portability across architectures, using domain-specific knowledge-level knowledge of the motif to optimize specific instantiations Where this fits in the ParLab ·ParLab is the interdisciplinary group at UC Berkeley investigating the impact of many-core parallelism ·Software stack is divided

  11. Par Pond vegetation status summer 1995 - July survey descriptive summary

    Microsoft Academic Search

    H. E. Jr. Mackey; R. S. Riley

    1995-01-01

    A survey of the emergent shoreline aquatic plant, communities began in June 1995, three months after the refilling of Par Pond to approximately 200 feet (61 meters) above mean sea level, and continued with this July survey. Aquatic plant communities, similar to the pre-drawdown Par Pond communities, are becoming reestablished. Beds of maidencane (Panicum hemitomon), lotus (Nelumbo lutea), water lily

  12. Quelques exercises 1. Soit la surface delimitee en dessous par l'axe des x au-dessus par la courbe

    E-print Network

    Katalin, Nagy

    Quelques exercises 1. Soit la surface d´elimit´ee en dessous par l'axe des x au-dessus par la engendr´e par la rotation de cette surface autour de l'axe des x. 2. Soit la surface d´elimit´ee en dessous par l'axe des x au-dessus par la courbe y = 1 x ln x , e x

  13. Quelques exercises 1. Soit la surface delimitee en dessous par l'axe des x au-dessus par la courbe

    E-print Network

    Katalin, Nagy

    Quelques exercises 1. Soit la surface d´elimit´ee en dessous par l'axe des x au-dessus par la surface autour de l'axe des x. 2. Soit la surface d´elimit´ee en dessous par l'axe des x au-dessus par la engendr´e par la rotation de cette surface autour de l'axe des x. 3. Soit la surface d´elimit´ee en

  14. Dissection of the region of Pseudomonas aeruginosa ParA that is important for dimerization and interactions with its partner ParB

    PubMed Central

    Bartosik, Aneta A.; Glabski, Krzysztof; Jecz, Paulina; Lasocki, Krzysztof; Mikosa, Malgorzata; Plochocka, Danuta; Thomas, Christopher M.

    2014-01-01

    Pseudomonas aeruginosa ParA belongs to a large subfamily of Walker-type ATPases acting as partitioning proteins in bacteria. ParA has the ability to both self-associate and interact with its partner ParB. Analysis of the deletion mutants defined the part of the protein involved in dimerization and interactions with ParB. Here, a set of ParA alanine substitution mutants in the region between E67 and L85 was created and analysed in vivo and in vitro. All mutants impaired in dimerization (substitutions at positions M74, H79, Y82 and L84) were also defective in interactions with ParB, suggesting that ParA–ParB interactions depend on the ability of ParA to dimerize. Mutants with alanine substitutions at positions E67, C68, L70, E72, F76, Q83 and L85 were not impaired in dimerization, but were defective in interactions with ParB. The dimerization interface partly overlapped the pseudo-hairpin, involved in interactions with ParB. ParA mutant derivatives tested in vitro showed no defects in ATPase activity. Two parA alleles (parA84, whose product can neither self-interact nor interact with ParB, and parA67, whose product is impaired in interactions with ParB, but not in dimerization) were introduced into the P. aeruginosa chromosome by homologous gene exchange. Both mutants showed defective separation of ParB foci, but to different extents. Only PAO1161 parA84 was visibly impaired in terms of chromosome segregation, growth rate and motility, similar to a parA-null mutant. PMID:25139949

  15. Recombination in the Human Pseudoautosomal Region PAR1

    PubMed Central

    Hinch, Anjali G.; Altemose, Nicolas; Noor, Nudrat; Donnelly, Peter; Myers, Simon R.

    2014-01-01

    The pseudoautosomal region (PAR) is a short region of homology between the mammalian X and Y chromosomes, which has undergone rapid evolution. A crossover in the PAR is essential for the proper disjunction of X and Y chromosomes in male meiosis, and PAR deletion results in male sterility. This leads the human PAR with the obligatory crossover, PAR1, to having an exceptionally high male crossover rate, which is 17-fold higher than the genome-wide average. However, the mechanism by which this obligatory crossover occurs remains unknown, as does the fine-scale positioning of crossovers across this region. Recent research in mice has suggested that crossovers in PAR may be mediated independently of the protein PRDM9, which localises virtually all crossovers in the autosomes. To investigate recombination in this region, we construct the most fine-scale genetic map containing directly observed crossovers to date using African-American pedigrees. We leverage recombination rates inferred from the breakdown of linkage disequilibrium in human populations and investigate the signatures of DNA evolution due to recombination. Further, we identify direct PRDM9 binding sites using ChIP-seq in human cells. Using these independent lines of evidence, we show that, in contrast with mouse, PRDM9 does localise peaks of recombination in the human PAR1. We find that recombination is a far more rapid and intense driver of sequence evolution in PAR1 than it is on the autosomes. We also show that PAR1 hotspot activities differ significantly among human populations. Finally, we find evidence that PAR1 hotspot positions have changed between human and chimpanzee, with no evidence of sharing among the hottest hotspots. We anticipate that the genetic maps built and validated in this work will aid research on this vital and fascinating region of the genome. PMID:25033397

  16. Signal Processing 86 (2006) 38903903 A low complexity multicarrier PAR reduction approach based

    E-print Network

    Erdogan, Alper Tunga

    2006-01-01

    on subgradient optimization$ Alper Tunga Erdoganà Electrical-Electronics Engineering, Koc University power ratio (PAR); PAPR; PAR reduction; PMEPR; Subgradient; Subdifferential; Tone reservation; VDSL 1

  17. COMPARAISON DE LA DTECTION DES FOYERS DE FASCIOLOSE PAR TEST ELISA SUR LACTOSRUM ET SRUM ET PAR COPROSCOPIE

    E-print Network

    Boyer, Edmond

    COMPARAISON DE LA DÉTECTION DES FOYERS DE FASCIOLOSE PAR TEST ELISA SUR LACTOSÉRUM ET SÉRUM ET PAR Cedex, France. Summary COMPARISON OF TECHNIQUES ELISA AND FECAL EXAMINATIONS TO DIAGNOSE HERDS INFEC and immunodiagnosis by ELISA technique were performed. Antibodies were searched for in the pooled milk of each herd

  18. SPRY1 promotes the degradation of uPAR and inhibits uPAR-mediated cell adhesion and proliferation

    PubMed Central

    Liu, Xiufeng; Lan, Yan; Zhang, Di; Wang, Kai; Wang, Yao; Hua, Zi-Chun

    2014-01-01

    Urokinase plasminogen activator receptor (uPAR) is a GPI anchored cell surface protein that is closely associated with invasion, migration, and metastasis of cancer cells. Many functional extracellular proteins and transmembrane receptors interact with uPAR. However, few studies have examined the association of uPAR with cytoplasm proteins. We previously used yeast two-hybrid screening to isolate several novel uPAR-interacting cytoplasmic proteins, including Sprouty1 (SPRY1), an inhibitor of the (Ras-mitogen-activated protein kinase) MAPK pathway. In this study, we show that SPRY1 interacts with uPAR and directs it toward lysosomal-mediated degradation. Overexpression of SPRY1 decreased the cell surface and cytoplasmic uPAR protein level. Moreover, SPRY1 overexpression augmented uPAR-induced cell adhesion to vitronectin as well as proliferation of cancer cells. Our results also further support the critical role of SPRY1 contribution to tumor growth. In a subcutaneous tumor model, overexpression of SPRY1 in HCT116 or A549 xenograft in athymic nude mice led to great suppression of tumor growth. These results show that SPRY1 may affect tumor cell function through direct interaction with uPAR and promote its lysosomal degradation. PMID:25520860

  19. INFECTION EXPRIMENTALE DU PORC A L'ENGRAIS PAR LE ROTAVIRUS: TUDE DE L'IMMUNIT LOCALE PAR LES

    E-print Network

    Paris-Sud XI, Université de

    INFECTION EXPÉRIMENTALE DU PORC A L'ENGRAIS PAR LE ROTAVIRUS: ÉTUDE DE L'IMMUNITÉ LOCALE PAR LES Porcine, 37380 Monnaie, France Summary EXPERIMENTAL INFECTION OF FATTENED PIGS WITH ROTAVIRUS: ANTIGENS in the faeces. Les rotavirus (reoviridae) sont associés à de nombreux syndromes entéritiques (Flewett et Woode

  20. Maladie de Horton révélée par une dyspnée

    PubMed Central

    Mahfoudhi, Madiha; Mamlouk, Habiba; Turki, Sami; Kheder, Adel

    2015-01-01

    Les manifestations pleuro-pulmonaires de la maladie de Horton sont rares et peu connues. Elles peuvent être inaugurales, à l'origine d'un retard à la prise en charge si elles sont méconnues. Il s'agissait d'un patient âgé de 75 ans, admis pour une dyspnée, une toux chronique et une fièvre. Il a reçu une antibiothérapie et a bénéficié d'une fibroscopie bronchique avec lavage broncho-alvéolaire à la recherche d'un germe, qui a révélé plutôt, une alvéolite lymphocytaire. L’évolution était marquée par la persistance des signes cliniques et du syndrome inflammatoire biologique. Un angio-scanner thoracique et une échographie cardiaque étaient sans anomalies. Une origine cardiaque, musculaire, hématologique, néoplasique, vasculaire ou métabolique de la dyspnée a été éliminée. Une maladie de Horton a été évoquée. La biopsie de l'artère temporale a confirmé le diagnostic d'une maladie de Horton. L’évolution sous corticothérapie était marquée par la disparition des signes cliniques et biologiques. Les manifestations pleuro-pulmonaires au cours de la maladie de Horton sont rares, et classiquement, rarement révélatrices de la maladie. La dyspnée peut initialement égarer le diagnostic vers d'autres étiologies notamment infectieuses. Le but de ce travail est d'insister sur le fait que la connaissance de ces différentes manifestations respiratoires au cours de la maladie de Horton (toux persistante, dyspnée, épanchement pleural) est utile au clinicien afin de prescrire une corticothérapie, chez un sujet le plus souvent âgé ayant un état fébrile et inflammatoire prolongé, permettant ainsi d’éviter l'apparition de complications. PMID:26161171

  1. Reconstituting ParA/ParB-mediated transport of DNA cargo.

    PubMed

    Vecchiarelli, Anthony G; Taylor, James A; Mizuuchi, Kiyoshi

    2015-01-01

    Protein gradients play key roles in subcellular spatial organization. In bacteria, ParA adenosine triphosphatases, or ATPases, form dynamic gradients on the nucleoid surface, which imparts positional information for the segregation, transport, and positioning of chromosomes, plasmids, and large protein assemblies. Despite the apparent simplicity of these minimal and self-organizing systems, the mechanism remains unclear. The small size of bacteria along with the number of physical and biochemical processes involved in subcellular organization makes it difficult to study these systems under controlled conditions in vivo. We developed a cell-free reconstitution technique that allows for the visualization of ParA-mediated cargo transport on a DNA carpet, which acts as a biomimetic of the nucleoid surface. Here, we present methods to express, purify, and visualize the dynamic properties of the SopABC system from F plasmid, considered a paradigm for the study of ParA-type systems. We hope similar cell-free studies will be used to address the biochemical and biophysical underpinnings of this ubiquitous transport scheme in bacteria. PMID:25997351

  2. Paraplégie compliquant une plaie abdominale antérieure par arme blanche

    PubMed Central

    Elahmadi, Brahim; Awab, Almahdi; El Moussaoui, Rachid; El Hijri, Ahmed; Azzouzi, Abderrahim; Alilou, Mustapha

    2015-01-01

    Les traumatismes médullaires sont des complications rares des plaies abdominales antérieures par arme blanche. Son diagnostic est difficile parfois retardé. L'imagerie par résonance magnétique reste l'examen de choix. Le traitement dépend du tableau clinique et de la gravité de la souffrance médullaire. Le pronostic est corrélé à l’étendue et à la nature de la lésion médullaire. Nous rapportons un cas exceptionnel d'un traumatisme médullaire chez une patiente victime d'une plaie abdominale antérieure par arme blanche. PMID:25995808

  3. 1 Cours de M.RUMIN rcrit par J.KULCSAR Chapitre IV

    E-print Network

    Rumin, Michel

    1 Cours de M.RUMIN réécrit par J.KULCSAR Chapitre IV Bases et dimension d'un espace vectoriel. Démonstration : () : est génératrice par hypothèse. est elle libre ? Soient tels que . #12;2 Cours de M.RUMIN vecteurs. #12;3 Cours de M.RUMIN réécrit par J.KULCSAR - Base d'un plan de défini par une équation

  4. Temprature de surface du Plateau Antarctique Cadre de simulations par le modle de neige Crocus

    E-print Network

    Ribes, Aurélien

    Température de surface du Plateau Antarctique Cadre de simulations par le modèle de neige Crocus BSRN (PNRA ENEA / ISAC-CNR) #12;5 Modèle de neige Crocus (Brun et al., 1992, Vionnet et al., 2012) Ts surface à Dôme C : - par Crocus forcé par les conditions météorologiques observées in-situ - par Crocus en

  5. The signaling adapter Gab1 regulates cell polarity by acting as a PAR protein scaffold

    PubMed Central

    Yang, Ziqiang; Xue, Bin; Umitsu, Masataka; Ikura, Mitsuhiko; Muthuswamy, Senthil K.; Neel, Benjamin G.

    2012-01-01

    Summary Cell polarity plays a key role in development and is disrupted in tumors, yet the molecules and mechanisms that regulate polarity remain poorly defined. We found that the scaffolding adaptor GAB1 interacts with two polarity proteins, PAR1 and PAR3. GAB1 binds PAR1 and enhances its kinase activity. GAB1 brings PAR1 and PAR3 into a transient complex, stimulating PAR3 phosphorylation by PAR1. GAB1 and PAR6 bind the PAR3 PDZ1 domain and thereby compete for PAR3 binding. Consequently, GAB1 depletion causes PAR3 hypo-phosphorylation and increases PAR3/PAR6 complex formation, resulting in accelerated and enhanced tight junction formation, increased trans-epithelial resistance and lateral domain shortening. Conversely, GAB1 over-expression, in a PAR1/PAR3-dependent manner, disrupts epithelial apical-basal polarity, promotes multi-lumen cyst formation, and enhances growth factor-induced epithelial cell scattering. Our results identify GAB1 as a novel negative regulator of epithelial cell polarity that functions as a scaffold for modulating PAR protein complexes on the lateral membrane. PMID:22883624

  6. View from northeast to southwest of PAR site sentry station; ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View from northeast to southwest of PAR site sentry station; formerly the bachelor's enlisted men's quarter (BEQ) - Stanley R. Mickelsen Safeguard Complex, Sentry Station, North of Second Avenue & West of Electrical Switch Station No. 2, Nekoma, Cavalier County, ND

  7. View from southeast to northwest of PAR site bachelor officers' ...

    Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey

    View from southeast to northwest of PAR site bachelor officers' quarters - Stanley R. Mickelsen Safeguard Complex, Bachelor Officers' Quarters, North of Second Avenue; South of Metal & Woodworking Shop No. 706, Nekoma, Cavalier County, ND

  8. PARS: Programs for Analysis and Resizing of Structures, user manual

    NASA Technical Reports Server (NTRS)

    Haftka, R. T.; Prasad, B.; Tsach, U.

    1979-01-01

    PARS processors and their use, flutter analysis, sensitivity analysis for stresses, and resizing are presented. Design variable definition and interface with finite element model, static constraints and their derivatives, flutter derivatives, and optimization are discussed.

  9. Ordonnancement de threads dirig par la mmoire sur architecture NUMA

    E-print Network

    Boyer, Edmond

    . Bien que la finesse de gravure continue de progresser, réduisant de plus en plus la taille des circuits'espace offert par une gravure plus fine pour ajouter des unités fonc- tionnelles ou des niveaux de caches

  10. Activation des cellules Natural Killer par les endocannabinodes

    E-print Network

    Activation des cellules Natural Killer par les endocannabinoïdes Anandamide et 2-Arachidonoyl Abréviations 6 Introduction 8 1. Les cellules Natural Killer 8 1.1. Les différents types de cellules NK : CD56

  11. Par Alain Ricard Explorateurs europens en Afrique noire

    E-print Network

    Paris-Sud XI, Université de

    ; les frères Lander n'ont pas découvert le Niger, mais ils ont situé son embouchure, établissant un lien entre un delta envahi par la mangrove et les " huiles " et un grand fleuve paresseux qui coulait en

  12. Un lymphangiome kystique rétropéritonéal révélé par des coliques néphrétiques

    PubMed Central

    El anzaoui, Jihad; Ghoundale, Omar; Touiti, Driss; El barni, Rachid; Mejdane, Abdelhadi; Fihri, Jawad Fassi; Lahkim, Mohammed; Achour, Abdsamad

    2015-01-01

    Résumé Le lymphangiome kystique (LK) est une tumeur rare des voies lymphatiques caractérisée par son évolution bénigne. La fréquence de la localisation rétropéritonéale est controversée. La découverte se fait le plus souvent dès l’enfance. L’évolution de la maladie peut être marquée par des complications graves, notamment compressives. Nous faisons état de l’observation médicale d’une femme de 30 ans chez qui l’on a découvert, à la suite de l’apparition de coliques néphrétiques récidivantes, un LK énorme occupant tout l’espace rétropéritonéal droit. Le diagnostic a été évoqué par l’urotomodensitométrie et confirmé par l’histologie. L’évolution après exérèse chirurgicale de la masse a été favorable, avec la disparition des coliques néphrétiques. PMID:25844099

  13. Les taxis pigs par 36 000 communes Richard Darbra

    E-print Network

    Paris-Sud XI, Université de

    non emergency patient transport or from adopting new technologies like smartphone malades ou d'adopter les nouvelles technologies de réservation par applications de téléphonie mobile. Abstract "The French taxis trapped by 36,000 local governments

  14. cole doctorale EMMA Lorraine Nattribu par la bibliothque

    E-print Network

    Boyer, Edmond

    �cole doctorale EMMA Lorraine N°attribué par la bibliothèque THESE Présentée et soutenue Lorraine Spécialité "Mécanique et �nergétique" Par Nadhir LEBAAL Optimisation de la tête d'extrusion pour R&D Suisse. Institut National Polytechnique de Lorraine-INPL Nancy 2, Avenue de la Forêt de Haye

  15. StePar: an automatic code for stellar parameter determination

    NASA Astrophysics Data System (ADS)

    Tabernero, H. M.; González Hernández, J. I.; Montes, D.

    2013-05-01

    We introduce a new automatic code (StePar) for determinig stellar atmospheric parameters (T_{eff}, log{g}, ? and [Fe/H]) in an automated way. StePar employs the 2002 version of the MOOG code (Sneden 1973) and a grid of Kurucz ATLAS9 plane-paralell model atmospheres (Kurucz 1993). The atmospheric parameters are obtained from the EWs of 263 Fe I and 36 Fe II lines (obtained from Sousa et al. 2008, A&A, 487, 373) iterating until the excitation and ionization equilibrium are fullfilled. StePar uses a Downhill Simplex method that minimizes a quadratic form composed by the excitation and ionization equilibrium conditions. Atmospheric parameters determined by StePar are independent of the stellar parameters initial-guess for the problem star, therefore we employ the canonical solar values as initial input. StePar can only deal with FGK stars from F6 to K4, also it can not work with fast rotators, veiled spectra, very metal poor stars or Signal to noise ratio below 30. Optionally StePar can operate with MARCS models (Gustafson et al. 2008, A&A, 486, 951) instead of Kurucz ATLAS9 models, additionally Turbospectrum (Alvarez & Plez 1998, A&A, 330, 1109) can replace the MOOG code and play its role during the parameter determination. StePar has been used to determine stellar parameters for some studies (Tabernero et al. 2012, A&A, 547, A13; Wisniewski et al. 2012, AJ, 143, 107). In addition StePar is being used to obtain parameters for FGK stars from the GAIA-ESO Survey.

  16. Another application for trellis shaping: PAR reduction for DMT (OFDM)

    Microsoft Academic Search

    Werner Henkel; Björn Wagner

    2000-01-01

    A bound for the possible reduction of the peak-to-average ratio (PAR) dependent on the rate as well as possible practical procedures are presented. The idea of trellis shaping, originally used to minimize average transmit power in single-carrier systems, is applied to the problem of PAR reduction in multicarrier transmission. Its impact, as a function of code rate, as well as

  17. Par reduction in ofdm via active constellation extension

    Microsoft Academic Search

    Brian Scott Krongold; Douglas L. Jones

    2003-01-01

    The high peak-to-average power ratio (PAR) in orthogonal frequency division multiplexing (OFDM) modulation systems can significantly reduce power efficiency and performance. Methods exist which alter or introduce new signal constellations to combat large signal peaks. We present a new PAR-reduction method that dynamically extends outer constellation points in active (data-carrying) channels, within margin-preserving constraints, in order to minimize the peak

  18. A distinct PAR complex associates physically with VE-cadherin in vertebrate endothelial cells

    Microsoft Academic Search

    Sandra Iden; Daniela Rehder; Benjamin August; Atsushi Suzuki; Karen Wolburg-Buchholz; Hartwig Wolburg; Shigeo Ohno; Jürgen Behrens; Dietmar Vestweber; Klaus Ebnet

    2006-01-01

    A cell polarity complex consisting of partitioning defective 3 (PAR-3), atypical protein kinase C (aPKC) and PAR-6 has a central role in the development of cell polarity in epithelial cells. In vertebrate epithelial cells, this complex localizes to tight junctions. Here, we provide evidence for the existence of a distinct PAR protein complex in endothelial cells. Both PAR-3 and PAR-6

  19. A mathematical model of ParA filament-mediated chromosome movement in Caulobacter crescentus.

    PubMed

    Shtylla, Blerta; Keener, James P

    2012-08-21

    Caulobacter crescentus uses the dynamic interactions between ParA and ParB proteins to segregate copies of its circular chromosome. In this paper, we develop two mathematical models of the movement of the circular chromosome of this bacterium during division. In the first model, posed as a set of stochastic differential equations (SDE), we propose that a simple biased diffusion mechanism for ParB/ParA interactions can reproduce the observed patterns of ParB and ParA localization in the cell. The second model, posed as a set of nonlinear partial differential equations, is a continuous treatment of the problem where we use results from the SDE model to describe ParB/ParA interactions and we also track ParA monomer dynamics in the cytoplasm. For both models, we show that if ParB complexes bind weakly and nonspecifically to ParA filaments, then they can closely track and move with the edge of a shrinking ParA filament bundle. Unidirectional chromosome movement occurs when ParB complexes have a passive role in depolymerizing ParA filaments. Finally, we show that tight control of ParA filament dynamics is essential for proper segregation. PMID:22588023

  20. Quelques exercises 1. Soit la surface delimitee en dessous par l'axe des x au-dessus par la courbe

    E-print Network

    Katalin, Nagy

    Quelques exercises 1. Soit la surface d´elimit´ee en dessous par l'axe des x au-dessus par la surface autour de l'axe des x. 2. Soit la surface d´elimit´ee en dessous par l'axe des x au-dessus par la cette surface autour de l'axe des x. 3. Tracer la graphe de la courbe y = 1 - x et prendre l'arc fini

  1. MESURE DU NOMBRE MOYEN DE NEUTRONS MIS PAR FISSION INDUITE DANS 238U PAR DES NEUTRONS DE 14,2 MeV

    E-print Network

    Boyer, Edmond

    66. MESURE DU NOMBRE MOYEN DE NEUTRONS ÉMIS PAR FISSION INDUITE DANS 238U PAR DES NEUTRONS DE 14 avons mesuré le rapport 03BD/03BD', du nombre moyen de neutrons 03BD émis par fission de 238U avec des neutrons de 14,2 MeV, au nombre de neutrons v' émis par fission de 235U avec des neutrons thermiques

  2. Evaluation de l'exposition humaine au plomb par ingestion et inhalation de PM10 et PM2,5 mises par une usine de recyclage de batteries

    E-print Network

    Paris-Sud XI, Université de

    Evaluation de l'exposition humaine au plomb par ingestion et inhalation de PM10 et PM2,5 émises par plomb et autres métaux par deux voies d'exposition : l'inhalation et l'ingestion. Dans le but de : elles représentent non seulement la fraction inhalable mais contribuent probablement à une fraction non

  3. Diffusion par le deutrium des neutrons de 14,1 MeV dans une direction inclrne de 30 par rapport

    E-print Network

    Boyer, Edmond

    803 FIG. 5. - Diffusion par le deutérium des neutrons de 14,1 MeV dans une direction inclrnée de 30° par rapport à celle des neutrons incidents. quement utilise une méthode de temps de vol entre les neutrons dans le compteur à scintil- lation équipé d'un plastique. Les impulsions déli- vrées par ce

  4. The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA

    PubMed Central

    Ebersbach, Gitte; Gerdes, Kenn

    2001-01-01

    Prokaryotic plasmids and chromosomes encode partitioning (par) loci that segregate DNA to daughter cells before cell division. Recent database analyses showed that almost all known par loci encode an ATPase and a DNA-binding protein, and one or more cis-acting regions where the proteins act. All par-encoded ATPases belong to one of two protein superfamilies, Walker-type and actin-like ATPases. This property was recently used to divide par loci into Types I and II loci. We show here that the Escherichia coli virulence factor pB171 encodes a double par locus that consists of one Type I and one Type II locus. Separately, each locus stabilized a test-plasmid efficiently. Together, the two loci mediated even more efficient plasmid stabilization. The par loci have a unique genetic organization in that they share a common central region at which the two different DNA-binding proteins probably act. Interestingly, a fusion protein consisting of the Walker-type ParA ATPase and Gfp was functional and oscillated in nucleoid regions on a time scale of minutes. ParA-green fluorescent protein (Gfp) oscillation depended on both ParB and parC but was independent of minCDE. Point mutations in the Walker A box motif simultaneously abolished plasmid stabilization and ParA-Gfp oscillation. These observations raise the possibility that ParA oscillation is prerequisite for active plasmid segregation. PMID:11752455

  5. Par Pond vegetation status Summer 1995 -- June survey descriptive summary

    SciTech Connect

    Mackey, H.E. Jr.; Riley, R.S.

    1995-06-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the shoreline aquatic plant communities in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level, indicated that much of the original plant communities and the intermediate shoreline communities present on the exposed sediments have been lost. The extensive old-field and emergent marsh communities that were present on the exposed shoreline during the drawdown have been flooded and much of the pre-drawdown Par Pond aquatic plant communities have not had sufficient time for re-establishment. The shoreline does, however, have extensive beds of maidencane which extend from the shoreline margin to areas as deep as 2 and perhaps 3 meters. Scattered individual plants of lotus and watershield are common and may indicate likely directions of future wetland development in Par Pond. In addition, within isolated coves, which apparently received ground water seepage and/or stream surface flows during the period of the Par Pond draw down, extensive beds of waterlilies and spike rush are common. Invasion of willow and red maple occurred along the lake shoreline as well. Although not absent from this survey, evidence of the extensive redevelopment of the large cattail and eel grass beds was not observed in this first survey of Par Pond. Future surveys during the growing seasons of 1995, 1996, and 1997 along with the evaluation of satellite date to map the areal extent of the macrophyte beds of Par Pond are planned.

  6. La dpense minima serait, dans des conditions moyennes, de ofr,002 par heure et par candie, soit environ of1',032 par heure

    E-print Network

    Boyer, Edmond

    dans le verre et dans le spath d'Islande avec la température); Wied. Ann., t. XXV, p. 87;I885. Dans le verre et le spath d'Islande, les indices croissent en même temps que la température. Les mesures celui que Fresnel a imaginé pour le quartz. Une longue auge de verre (0m,54 de long) fu t partagée par

  7. Both PHYTOCHROME RAPIDLY REGULATED1 (PAR1) and PAR2 Promote Seedling Photomorphogenesis in Multiple Light Signaling Pathways1[C][W][OPEN

    PubMed Central

    Zhou, Peng; Song, Meifang; Yang, Qinghua; Su, Liang; Hou, Pei; Guo, Lin; Zheng, Xu; Xi, Yulin; Meng, Fanhua; Xiao, Yang; Yang, Li; Yang, Jianping

    2014-01-01

    Arabidopsis (Arabidopsis thaliana) seedlings undergo photomorphogenesis in the light and etiolation in the dark. Light-activated photoreceptors transduce the light signals through a series of photomorphogenesis promoting or repressing factors to modulate many developmental processes in plants, such as photomorphogenesis and shade avoidance. CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) is a conserved RING finger E3 ubiquitin ligase, which mediates degradation of several photomorphogenesis promoting factors, including ELONGATED HYPOCOTYL5 (HY5) and LONG HYPOCOTYL IN FAR-RED1 (HFR1), through a 26S proteasome-dependent pathway. PHYTOCHROME RAPIDLY REGULATED1 (PAR1) was first detected as an early repressed gene in both phytochrome A (phyA)-mediated far-red and phyB-mediated red signaling pathways, and subsequent studies showed that both PAR1 and PAR2 are negative factors of shade avoidance in Arabidopsis. However, the role of PAR1 and PAR2 in seedling deetiolation, and their relationships with other photomorphogenesis promoting and repressing factors are largely unknown. Here, we confirmed that both PAR1 and PAR2 redundantly enhance seedling deetiolation in multiple photoreceptor signaling pathways. Their transcript abundances are repressed by phyA, phyB, and cryptochrome1 under far-red, red, and blue light conditions, respectively. Both PAR1 and PAR2 act downstream of COP1, and COP1 mediates the degradation of PAR1 and PAR2 through the 26S proteasome pathway. Both PAR1 and PAR2 act in a separate pathway from HY5 and HFR1 under different light conditions, except for sharing in the same pathway with HFR1 under far-red light. Together, our results substantiate that PAR1 and PAR2 are positive factors functioning in multiple photoreceptor signaling pathways during seedling deetiolation. PMID:24335334

  8. Par Pond vegetation status Summer 1995 -- October survey descriptive summary

    SciTech Connect

    Mackey, H.E. Jr.; Riley, R.S.

    1995-11-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the emergent shoreline aquatic plant communities began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level and continued with this late October survey. Communities similar to the pre-drawdown Par Pond aquatic plant communities are becoming re-established; especially, beds of maiden cane, lotus, waterlily, and watershield are now extensive and well established. Cattail occurrence continues to increase, but large beds common to Par Pond prior to the drawdown have not formed. Future surveys throughout 1996 and 1997, along with the continued evaluation of satellite data to map the areal extent of the macrophyte beds of Par Pond, are planned.

  9. Image algebra preprocessor for the MasPar parallel computer

    NASA Astrophysics Data System (ADS)

    Meyer, Trevor E.; Davidson, Jennifer L.

    1991-07-01

    The development of a preprocessor for image algebra on the MasPar computer, a SIMD processor array, is discussed. One of the parallel languages used on the MasPar is MPL, a parallel version of the programming language C. This machine and language were chosen because of the close correspondence between MPL and image algebra and, as a result, MPL is easily extended to include image algebra. The preprocessor consists of three primary components: the lexical analyzer, the parser, and the code generator. The lexical analyzer converts the input stream into tokens the parser recognizes. The parser checks the program for syntax errors.The code generator produces MPL source code which is then compiled and run on the MasPar. The architecture of the MasPar computer is reviewed, and in particular the structures used for routing data through the array are examined. The parallel language MPL is also reviewed, with attention given to the methods in which the extensions to C in MPL interact with the MasPar architecture. The close correspondence between MPL and image algebra is specifically discussed. The authors present the structure and development of the image algebra preprocessor, including possible future extensions. Examples of image algebra preprocessor code and the corresponding MPL code produced by the preprocessor are given.

  10. Par Pond vegetation status Summer 1995 -- September survey descriptive summary

    SciTech Connect

    Mackey, H.E. Jr.; Riley, R.S.

    1995-09-01

    The water level of Par Pond was lowered approximately 20 feet in mid-1991 in order to protect downstream residents from possible dam failure suggested by subsidence on the downstream slope of the dam and to repair the dam. This lowering exposed both emergent and nonemergent macrophyte beds to drying conditions resulting in extensive losses. A survey of the emergent shoreline aquatic plant communities began in June 1995, three months after the refilling of Par Pond to approximately 200 feet above mean sea level and continued with this mid-September survey. Communities similar to the pre-drawdown Par Pond aquatic plant communities are becoming re-established; especially, beds of maidencane, lotus, waterlily, and watershield are now extensive and well established. Cattail occurrence continues to increase, but large beds common to Par Pond prior to the drawdown have not formed. Future surveys during the late growing seasons of 1995, and throughout 1996 and 1997, along with the evaluation of satellite data to map the areal extent of the macrophyte beds of Par Pond, are planned.

  11. Expression and function of the leucine zipper protein Par-4 in apoptosis.

    PubMed Central

    Sells, S F; Han, S S; Muthukkumar, S; Maddiwar, N; Johnstone, R; Boghaert, E; Gillis, D; Liu, G; Nair, P; Monnig, S; Collini, P; Mattson, M P; Sukhatme, V P; Zimmer, S G; Wood, D P; McRoberts, J W; Shi, Y; Rangnekar, V M

    1997-01-01

    The prostate apoptosis response-4 (par-4) gene was identified by differential screening for genes that are upregulated when prostate cancer cells are induced to undergo apoptosis. The par-4 gene is induced by apoptotic signals but not by growth-arresting, necrotic, or growth-stimulatory signals. The deduced amino acid sequence of par-4 predicts a protein with a leucine zipper domain at its carboxy terminus. We have recently shown that the Par-4 protein binds, via its leucine zipper domain, to the zinc finger domain of Wilms' tumor protein WT1 (R. W. Johnstone et al., Mol. Cell. Biol. 16:6945-6956, 1996). In experiments aimed at determining the functional role of par-4 in apoptosis, an antisense par-4 oligomer abrogated par-4 expression and activator-driven apoptosis in rat prostate cancer cell line AT-3, suggesting that par-4 is required for apoptosis in these cells. Consistent with a functional role for par-4 in apoptosis, ectopic overexpression of par-4 in prostate cancer cell line PC-3 and melanoma cell line A375-C6 conferred supersensitivity to apoptotic stimuli. Transfection studies with deletion mutants of Par-4 revealed that full-length Par-4, but not mutants that lacked the leucine zipper domain of Par-4, conferred enhanced sensitivity to apoptotic stimuli. Most importantly, ectopic coexpression of the leucine zipper domain of Par-4 inhibited the ability of Par-4 to enhance apoptosis. Finally, ectopic expression of WT1 attenuated apoptosis, and coexpression of Par-4 but not a leucine zipperless mutant of Par-4 rescued the cells from the antiapoptotic effect of WT1. These findings suggest that the leucine zipper domain is required for the Par-4 protein to function in apoptosis. PMID:9199316

  12. Protease activated receptor-2 (PAR2): possible target of phytochemicals.

    PubMed

    Kakarala, Kavita Kumari; Jamil, Kaiser

    2015-09-01

    The use of phytochemicals either singly or in combination with other anticancer drugs comes with an advantage of less toxicity and minimal side effects. Signaling pathways play central role in cell cycle, cell growth, metabolism, etc. Thus, the identification of phytochemicals with promising antagonistic effect on the receptor/s playing key role in single transduction may have better therapeutic application. With this background, phytochemicals were screened against protease-activated receptor 2 (PAR2). PAR2 belongs to the superfamily of GPCRs and is an important target for breast cancer. Using in silico methods, this study was able to identify the phytochemicals with promising binding affinity suggesting their therapeutic potential in the treatment of breast cancer. The findings from this study acquires importance as the information on the possible agonists and antagonists of PAR2 is limited due its unique mechanism of activation. PMID:25386994

  13. The centromere-like parC locus of plasmid R1.

    PubMed

    Breüner, A; Jensen, R B; Dam, M; Pedersen, S; Gerdes, K

    1996-05-01

    The parA partitioning system of plasmid R1 consists of three components: the cis-acting centromere-like parC locus, and two proteins, ParM and ParR. The parC locus contains two sets of five direct repeats (iterons) to which the ParR protein binds. The parA promoter is located in the core region between the two sets of iterons. Mini-R1 replicons carrying parC are stabilized by the simultaneous presence of ParM and ParR. The parC locus present on a co-resident plasmid leads to instability of the mini-R1 replicon (incompatibility). Here we present a genetic analysis of the stability and incompatibility phenotypes associated with parC. We show that all 10 iterons are required for maximum stabilization and incompatibility. Replacement of the core promoter region between the repeats by a foreign promoter region did not reduce stabilization. Thus, the only structural components in parC seem to be the two sets of iterons. The parA promoter, P parA, is repressed by ParR. We show that all 10 iterons are required for full repression of the promoter. The activity of the promoter was influenced by sequences located outside the core region. An A-rich region located upstream of the -35 element of PparA was found to increase promoter activity. The region encoding the parA mRNA leader region also strongly influenced the expression level of PparA- lacZ fusions. We show that this high expression (hex) element is a transcriptional antiterminator that prevents Rho-dependent termination. PMID:8736537

  14. Competing ParA Structures Space Bacterial Plasmids Equally over the Nucleoid

    PubMed Central

    Gerdes, Kenn; Howard, Martin

    2014-01-01

    Low copy number plasmids in bacteria require segregation for stable inheritance through cell division. This is often achieved by a parABC locus, comprising an ATPase ParA, DNA-binding protein ParB and a parC region, encoding ParB-binding sites. These minimal components space plasmids equally over the nucleoid, yet the underlying mechanism is not understood. Here we investigate a model where ParA-ATP can dynamically associate to the nucleoid and is hydrolyzed by plasmid-associated ParB, thereby creating nucleoid-bound, self-organizing ParA concentration gradients. We show mathematically that differences between competing ParA concentrations on either side of a plasmid can specify regular plasmid positioning. Such positioning can be achieved regardless of the exact mechanism of plasmid movement, including plasmid diffusion with ParA-mediated immobilization or directed plasmid motion induced by ParB/parC-stimulated ParA structure disassembly. However, we find experimentally that parABC from Escherichia coli plasmid pB171 increases plasmid mobility, inconsistent with diffusion/immobilization. Instead our observations favor directed plasmid motion. Our model predicts less oscillatory ParA dynamics than previously believed, a prediction we verify experimentally. We also show that ParA localization and plasmid positioning depend on the underlying nucleoid morphology, indicating that the chromosomal architecture constrains ParA structure formation. Our directed motion model unifies previously contradictory models for plasmid segregation and provides a robust mechanistic basis for self-organized plasmid spacing that may be widely applicable. PMID:25521716

  15. Modelling the Establishment of PAR Protein Polarity in the One-Cell C. elegans Embryo

    E-print Network

    Filipe Tostevin; Martin Howard

    2008-11-20

    At the one-cell stage, the C. elegans embryo becomes polarized along the anterior-posterior axis. The PAR proteins form complementary anterior and posterior domains in a dynamic process driven by cytoskeletal rearrangement. Initially, the PAR proteins are uniformly distributed throughout the embryo. Following a cue from fertilization, cortical actomyosin contracts towards the anterior pole. PAR-3/PAR-6/PKC-3 (the anterior PAR proteins) become restricted to the anterior cortex. PAR-1 and PAR-2 (the posterior PAR proteins) become enriched in the posterior cortical region. We present a mathematical model of this polarity establishment process, in which we take a novel approach to combine reaction-diffusion dynamics of the PAR proteins coupled to a simple model of actomyosin contraction. We show that known interactions between the PAR proteins are sufficient to explain many aspects of the observed cortical PAR dynamics in both wild-type and mutant embryos. However, cytoplasmic PAR protein polarity, which is vital for generating daughter cells with distinct molecular components, cannot be properly explained within such a framework. We therefore consider additional mechanisms that can reproduce the proper cytoplasmic polarity. In particular we predict that cytoskeletal asymmetry in the cytoplasm, in addition to the cortical actomyosin asymmetry, is a critical determinant of PAR protein localization.

  16. ParA-mediated plasmid partition driven by protein pattern self-organization

    PubMed Central

    Hwang, Ling Chin; Vecchiarelli, Anthony G; Han, Yong-Woon; Mizuuchi, Michiyo; Harada, Yoshie; Funnell, Barbara E; Mizuuchi, Kiyoshi

    2013-01-01

    DNA segregation ensures the stable inheritance of genetic material prior to cell division. Many bacterial chromosomes and low-copy plasmids, such as the plasmids P1 and F, employ a three-component system to partition replicated genomes: a partition site on the DNA target, typically called parS, a partition site binding protein, typically called ParB, and a Walker-type ATPase, typically called ParA, which also binds non-specific DNA. In vivo, the ParA family of ATPases forms dynamic patterns over the nucleoid, but how ATP-driven patterning is involved in partition is unknown. We reconstituted and visualized ParA-mediated plasmid partition inside a DNA-carpeted flowcell, which acts as an artificial nucleoid. ParA and ParB transiently bridged plasmid to the DNA carpet. ParB-stimulated ATP hydrolysis by ParA resulted in ParA disassembly from the bridging complex and from the surrounding DNA carpet, which led to plasmid detachment. Our results support a diffusion-ratchet model, where ParB on the plasmid chases and redistributes the ParA gradient on the nucleoid, which in turn mobilizes the plasmid. PMID:23443047

  17. An in vitro investigation of the actions of reproductive hormones on the cervix of the ewe in the follicular stage: the effects of 17?-estradiol, oxytocin, FSH, and arachidonic acid on the cervical pathway for the synthesis of prostaglandin E2.

    PubMed

    Falchi, L; Scaramuzzi, R J

    2015-04-01

    During the periovulatory period, the cervix of the ewe relaxes and this mechanism is thought to be mediated by oxytocin and prostaglandin E2 (PGE2) in response to increased concentrations of 17?-estradiol and perhaps FSH. The aim of the study was to determine the in vitro effects of 17?-estradiol, FSH, oxytocin, and arachidonic acid (AA) on the synthesis of PGE2 and on the expression of oxytocin receptor (OTR), cytoplasmic phospholipase A2 (cPLA2), and cyclooxygenase 2 (COX-2) in explants of cervical tissue collected from ewes in the periovulatory phase of the estrous cycle. Cervical minces from ewes in the follicular phase of the estrous cycle were cultured in supplemented Eagle's Minimum Essential Medium for 48 hours with 17?-estradiol, FSH, oxytocin, or AA. After incubation, the tissue was stored at -80 °C and the media at -20 °C. Western immunoblotting was used to determine relative levels of OTR, cPLA2, and COX-2 in cervical tissue, and the media was analyzed by RIA, to determine the concentration of PGE2. The addition of 17?-estradiol increased the concentration of PGE2 in the media (P = 0.001), the levels of COX-2 (P = 0.02) and OTR (P = 0.006) but not those of cPLA2 (P = 0.15). The addition of FSH increased the levels of COX-2 (P = 0.01) but, it had no effect on the concentration of PGE2 (P = 0.08) or on the levels of OTR (P = 0.07) and cPLA2 (P = 0.15). Oxytocin did not increase the levels of COX-2 (P = 0.38) but increased those of OTR (P = 0.001) and cPLA2 (P = 0.01) but not on the concentration of PGE2 in the media. Arachidonic acid increased the levels of cPLA2 (P = 0.01) and those of COX-2 (P = 0.02) but not the concentration of PGE2 in the media. Our findings suggest that the PGE2-mediated mechanisms of cervical relaxation in the ewe during the follicular phase are stimulated by FSH, 17?-estradiol, oxytocin, and AA. They all appear to act by inducing receptors and enzymes along the synthetic pathway for PGE2. PMID:25586640

  18. Subthalamic Stimulation-Induced Synaptic Responses in Substantia Nigra Pars Compacta Dopaminergic Neurons In Vitro

    E-print Network

    Tepper, James M.

    Subthalamic Stimulation-Induced Synaptic Responses in Substantia Nigra Pars Compacta Dopaminergic. Subthalamic stimulation-induced synaptic responses in sub- stantia nigra pars compacta dopaminergic neurons sources of excitatory glutamatergic input to dopaminer- gic neurons of the substantia nigra, yet

  19. PHNOMNES LECTRIQUES DES CRISTAUX HMIDRES A FACES INCLINES ; PAR MM. JACQUES ET PIERRE CURIE.

    E-print Network

    Paris-Sud XI, Université de

    par un exemple : les cris- taux de tourmaline ont un axe électrique qui coïncide avec l'axe principal'électricité sue les deux bases. Dans le cas de la tourmaline, les phénomènes électriques sont de même sens par

  20. Cette hypothse a t confirme par une srie d'essais. Des effets analogues

    E-print Network

    Paris-Sud XI, Université de

    provoqués par la vaseline au silicone, mais également par la lanoline. On n'a pas constaté de toxicité aiguë de la vaseline au silicone ou de la lanoline. Les abeilles transportent sur les rayons la graisse

  1. son tat molculaire primitif, par suite de l'existence de ce frotte-ment interne ;

    E-print Network

    Boyer, Edmond

    décrément logarithmique et par suite le frottement interne subissent des accroissements très rapides vers 55443 son état moléculaire primitif, par suite de l'existence de ce frotte- ment interne ; 2° Que le

  2. Par-4 downregulation promotes breast cancer recurrence by preventing multinucleation following targeted therapy.

    PubMed

    Alvarez, James V; Pan, Tien-Chi; Ruth, Jason; Feng, Yi; Zhou, Alice; Pant, Dhruv; Grimley, Joshua S; Wandless, Thomas J; Demichele, Angela; Chodosh, Lewis A

    2013-07-01

    Most deaths from breast cancer result from tumor recurrence, but mechanisms underlying tumor relapse are largely unknown. We now report that Par-4 is downregulated during tumor recurrence and that Par-4 downregulation is necessary and sufficient to promote recurrence. Tumor cells with low Par-4 expression survive therapy by evading a program of Par-4-dependent multinucleation and apoptosis that is otherwise engaged following treatment. Low Par-4 expression is associated with poor response to neoadjuvant chemotherapy and an increased risk of relapse in patients with breast cancer, and Par-4 is downregulated in residual tumor cells that survive neoadjuvant chemotherapy. Our findings identify Par-4-induced multinucleation as a mechanism of cell death in oncogene-addicted cells and establish Par-4 as a negative regulator of breast cancer recurrence. PMID:23770012

  3. The ParMRC system: molecular mechanisms of plasmid segregation by actin-like filaments

    Microsoft Academic Search

    Jeanne Salje; Pananghat Gayathri; Jan Löwe

    2010-01-01

    The ParMRC plasmid partitioning apparatus is one of the best characterized systems for bacterial DNA segregation. Bundles of actin-like filaments are used to push plasmids to opposite poles of the cell, whereupon they are stably inherited on cell division. This plasmid-encoded system comprises just three components: an actin-like protein, ParM, a DNA-binding adaptor protein, ParR, and a centromere-like region, parC.

  4. gulires signales par Buttgenbach et Gaubert [5] et qui se dvelopperaient ici lorsque les invitables

    E-print Network

    Paris-Sud XI, Université de

    -optique, appelée r41 par Cady [3] et e,, par Pockels, est donnée par la formule ([2], p. 67). E = V/d désignant le champ électrique, -,ri' la suscep-d tibilité électrique à tension constante (que Pockels désigne par x, 37I). [2] POCKELS F. - Abhandl. der Gesell. Wiss. Göttingen, I893, 39, 40. [3] CADY

  5. Hes1 regulates formations of the hypophyseal pars tuberalis and the hypothalamus

    Microsoft Academic Search

    Minekatsu Akimoto; Toshiyuki Nishimaki; Yuta Arai; Eiju Uchinuma; Hiroshi Yamauchi; Yoko Kameda

    2010-01-01

    The hypophyseal pars tuberalis surrounds the median eminence and infundibular stalk of the hypothalamus as thin layers of\\u000a cells. The pars tuberalis expresses MT1 melatonin receptor and participates in mediating the photoperiodic secretion of pituitary\\u000a hormones. Both the rostral tip of Rathke’s pouch (pars tuberalis primordium) and the pars tuberalis expressed ?GSU mRNA, and\\u000a were immunoreactive for LH, chromogranin A,

  6. ALCALOSE INDUITE PAR LE NITRATE CHEZ LA POULE

    E-print Network

    Paris-Sud XI, Université de

    ALCALOSE INDUITE PAR LE NITRATE CHEZ LA POULE ET CARACTÉRISTIQUES DE L'ŒUF B. SAUVEUR Mme H'alcalose chez les Mammifères, exerce la même action chez la Poule et modifie les caractéristiques de l'oeuf. Ce'oeuf les réponses varient suivant le génotype des animaux : chez des poules White Leghorn, la restriction

  7. L'valuation du prix des actions par les fondamentaux

    E-print Network

    Paris-Sud XI, Université de

    L'évaluation du prix des actions par les fondamentaux : analyse du marché français Dominique Pépin(*) Depuis 1920, le prix des actions en France, comme partout dans le monde, a connu d'importantes variations des banques centrales, et à se demander si ces dernières ne devaient pas prendre en compte le prix des

  8. SUR LA FLUORESCENCE ROUGE DU VERRE Par L. BRUNINGHAUS.

    E-print Network

    Boyer, Edmond

    SUR LA FLUORESCENCE ROUGE DU VERRE Par L. BRUNINGHAUS. Sommaire. - Cette note est consacrée à l. - Ayant eu récemment l'occasion de lire un mémoire de H. Peters sur la fluorescence rouge du verre, j cathodiques lents, des parois de verre contenant de l'oxygène occlus. E. Goldstein (2) indiqua en 1906 qu

  9. OSCILLATION D'UN JET LIQUIDE Par GUY LITTAYE.

    E-print Network

    Boyer, Edmond

    à l'extrémité d'un tube de verre court. Le liquide, l'eau en l'espèce, est amené au tube de verre par un tube de caoutchouc. Le tube de verre est fixé sur une lame d'acier qui effectue des observer exception- nellement un aspect semblable du jet alors que le tube de verre était soigneusement

  10. Traumatismes Oculaires par Petards: Bilan sur Trois Annees

    PubMed Central

    Zouaoui-Kesraoui, N.; Derdour, A.

    2009-01-01

    Summary Les accidents dus aux pétards sont des accidents graves. Leur recrudescence ces dernières années en Algérie, essentiellement durant les fêtes du Mawlid Ennabaoui (fête de la naissance du prophète), mérite à notre sens d'entreprendre des bilans exhaustifs dont celui-ci dans le but d'une sensibilisation de toutes les compétences concernées. Nous avons réuni sur trois années consécutives (2002, 2003, 2004) 60 dossiers de malades ayant subi des accidents oculaires par pétards. Nos patients sont répartis en 42 consultations pour blessures légères et 18 hospitalisations pour blessures graves. Parmi ces derniers, neuf ont présenté des complications et séquelles graves (cinq cas de cécité par atrophie du globe oculaire, trois cas de cécité cornéenne et un cas de cécité par trou maculaire). Dans tous ces cas l'incapacité permanente partielle est au minimum de 30%. Au vu de ces données nous proposons des mesures d'éducation sanitaire et une sensibilisation du grand public aux traumatismes oculaires, par le biais de mé dias appropriés: radio, télévision, affiches. PMID:21991157

  11. INFECTION EXPRIMENTALE DU BLIER PAR SALMONELLA ABORTUS OVIS

    E-print Network

    Paris-Sud XI, Université de

    INFECTION EXPÉRIMENTALE DU BÉLIER PAR SALMONELLA ABORTUS OVIS R. SANCHIS P. PARDON2 Georgette, France Summary EXPERIMENTAL INFECTION OF RAM WITH SALMONELLA ABORTUS OVIS. - The susceptibility of rams to experimental challenge with Salmonella abortus ovis was investigated by subcutaneous, conjunctival or preputial

  12. Leucémie aigüe myélomonocytaire à éosinophiles révélée par une pancréatite aigüe

    PubMed Central

    Touaoussa, Aziz; Elhmadi, Khalid; El Youssi, Hind; Moncef, Hichameddou; Hassani, Moncef Amrani

    2015-01-01

    La leucémie aigüe myélomonocytaire à composante éosinophile (LAM4eo) est une hémopathie maligne rare, caractérisée par une prolifération blastique myéloïde avec présence d'une composante monocytaire et des éosinophiles anormaux. Elle est associée à l'inversion du chromosome 16, parfois à sa variante la translocation (16;16). Nous rapportons un cas de LAM4eo chez un patient de sexe masculin âgé de 51 ans, découverte au décours d'un bilan paraclinique pour pancréatite aiguë (PA) confirmée par la TDM. L'hémogramme a montré une hyperleucocytose à 123G/l faite de 60% de blastes, une monocytose à 5 G/L ainsi qu'une lignée éosinophile dystrophique. Le myélogramme a objectivé l'infiltration de la moelle par une population blastique estimée à 61% d'expression hétérogène à la Cytométrie de flux: Des blastes très immatures exprimant fortement les marqueurs CD117 et CD34; des blastes prédominants qui expriment les antigènes CD33, CD13, CD65 (myéloblastes). Et une partie des blastes, était positive pour le CD14, CD4, CD11c (monoblastes). Après une exploration étiologique approfondie n'ayant pas pu trouver un lien de cause à effet, l'association entre les deux pathologies a été considérée comme fortuite et la pancréatite a été rattachée à la prise de paroxétine. Le patient a été mis alors en condition, et a été traité par chimiothérapie avec bonne évolution clinique et biologique. PMID:26090060

  13. DCLENCHEMENT DE LA MASTICATION PAR DISTENSION ŒSOPHAGIENNE CHEZ LE MOUTON

    E-print Network

    Boyer, Edmond

    DÉCLENCHEMENT DE LA MASTICATION PAR DISTENSION ŒSOPHAGIENNE CHEZ LE MOUTON R. BOIVIN, J. BOST IN SHEEP. ― !sophageal wall disten- sion was performed on standing conscious sheep by inflating of the regurgitated bolus into the mouth. La mastication est, le plus souvent, un acte réflexe qui suit l

  14. AIMANTATION SPONTANE DES FERRITES Par REN PAUTHENET et L. BOCHIROL.

    E-print Network

    Paris-Sud XI, Université de

    également étudié la variation thermique de l'aimantation spontanée des ferrites de nickel, de cobalt et deAIMANTATION SPONTANÉE DES FERRITES Par RENÉ PAUTHENET et L. BOCHIROL. Sommaire. 2014 Les auteurs ont étudié les variations de l'aimantation à saturation des ferrites de magnésium et de cuivre en

  15. ANOMALIE DE LONGUEUR DES FERRITES Par LOUIS WEIL.

    E-print Network

    Paris-Sud XI, Université de

    ferrites de cuivre, de nickel, de cobalt, de zinc et de magnésium, entre la température ordinaire et 900° CANOMALIE DE LONGUEUR DES FERRITES Par LOUIS WEIL. Sommaire. - L'auteur a étudié la dilatation des étudié au dilatomètre Chevenard divers ferrites. Lorsqu'on fait varier la température assez lentement

  16. Histoire(de(l'Astronomie( par(Thomas(Widemann((

    E-print Network

    Demoulin, Pascal

    (ses(observations.(Dans(son(oeuvre,( "l'Almageste",(ainsi(dénommée(par(les( Arabes,(il(présente(son(système( géocentrique(du(centre(de(l'épicycle(est(uniforme.(( Almageste (grec, IXe siècle)! Almageste (latin, XIII-XIVe siècle)! L'astronomie(araboIislamique( ( Parmi

  17. The Pars Triangularis in Dyslexia and ADHD: A Comprehensive Approach

    ERIC Educational Resources Information Center

    Kibby, Michelle Y.; Kroese, Judith M.; Krebbs, Hillery; Hill, Crystal E.; Hynd, George W.

    2009-01-01

    Limited research has been conducted on the structure of the pars triangularis (PT) in dyslexia despite functional neuroimaging research finding it may play a role in phonological processing. Furthermore, research to date has not examined PT size in ADHD even though the right inferior frontal region has been implicated in the disorder. Hence, one…

  18. Clinical and immunopathological studies of pars planitis in a family.

    PubMed Central

    Wetzig, R P; Chan, C C; Nussenblatt, R B; Palestine, A G; Mazur, D O; Mittal, K K

    1988-01-01

    We examined a family in which two brothers with identical HLA typing have pars planitis with snowbanking. Immunopathological studies of one of their eyes showed that in the area of snowbanking over the pars plana there was mild to moderate inflammatory cell infiltration, consisting of mostly Pan T (Leu 4+) lymphocytes. The ratio of T helper/inducer to T suppressor/cytotoxic cells was approximately 10:1. Few macrophages (OKM1+) were identified. Very few B cells and no NK cells were observed. Some retinal vessels had a perivascular infiltration consisting of mostly T lymphocytes. Most of the inflammatory cells bore class II antigens (HLA-DR+), while T cells bore few IL-2 receptors (anti-TAC+). The snowbank consisted mainly of glial elements (GFAP+) and basement membrane components (type IV collagen and laminin) with the predominant cell the Müller cell (Mü+). A site of inflammation at the iris-ciliary body junction also stained for B cells (Leu 14+). These findings suggest that the snowbank could be formed by the glial elements of the peripheral retina. The chronic inflammation in pars planitis appears to consist of helper T cells, both in the pars plana, and the retinal vasculature. Images PMID:3257703

  19. Berkeley ParLab Ten Ways to Waste a Parallel

    E-print Network

    Moreno Maza, Marc

    2005 2010 Transistors (in Thousands) Data from Kunle Olukotun, Lance Hammond, Herb Sutter, Burton Smith, Lance Hammond, Herb Sutter, Burton Smith, Chris Batten, and Krste Asanoviç #12;Berkeley Par) Frequency (MHz) Power (W) Perf Cores Data from Kunle Olukotun, Lance Hammond, Herb Sutter, Burton Smith

  20. INSTITUT NATIONAL POLYTECHNIQUE DE GRENOBLE N attribu par la bibliothque

    E-print Network

    Paris-Sud XI, Université de

    , Traitement du Signal présentée et soutenue publiquement par Luis ROLINDEZ le 23 février 2007 Titre : Technique d'auto test pour des convertisseurs de signal Sigma-Delta Directeur de thèse : Salvador MIR Co M. Andreas KAISER , Rapporteur M. Salvador MIR , Directeur de thèse M. Jean-Louis CARBONERO , Co

  1. Dtection de Pseudomonas phaseolicola (Burkh.) Dowson par la technique ELISA

    E-print Network

    Paris-Sud XI, Université de

    Détection de Pseudomonas phaseolicola (Burkh.) Dowson par la technique ELISA Marie-Renée BARZIC des feuilles contaminées de haricot. ELISA, Le test EusA a été réalisé selon la méthode sandwich d. phaseolicola comme ligand (fig. 1). Métabolites, ' Des études préalables ont montré que la technique ELISA

  2. Scheduling on the MasPar SIMD parallel computer

    E-print Network

    Perkins, Keith Douglas

    1995-01-01

    whose scheduling times are within 90% of optimal schedules 95% of the time. The highest level first algorithm was then modifies to handle the processor architecture on the Texas A&M University's MasPar parallel computer. These modifications included...

  3. Aqueous misdirection following pars plana vitrectomy and silicone oil injection

    PubMed Central

    Ghoraba, Hammouda H; Ghali, Ali Ahmed; Mansour, Hosam Othman

    2015-01-01

    Purpose To report a retrospective series of seven phakic eyes of seven patients suffering from a malignant glaucoma-like syndrome following pars plana vitrectomy and silicone oil (SO) injection. Materials and methods Seven eyes with retinal detachment treated with pars plana vitrectomy with or without scleral buckling with SO tamponade. This was followed by cataract extraction to manage the elevated intraocular pressure (IOP). Results This was a retrospective review of seven cases that received pars plana vitrectomy and SO with or without scleral buckling for different causes of retinal detachment (three were rhegmatogenous and four were tractional). After a period ranging from 1 week to 1 month, they presented with malignant glaucoma-like manifestations; high IOP, shallow axial anterior chamber, and remarkable decrease of visual acuity. Atropine eye drops and anti-glaucoma medical treatment (topical and systemic) had been tried but failed to improve the condition. Dramatic decrease of IOP and deepening of the axial anterior chamber was observed in all cases in the first postoperative day after phacoemulsification and posterior chamber foldable intraocular lens implantation with posterior capsulotomy. Conclusion Aqueous misdirection syndrome may be observed following pars plana vitrectomy and SO tamponade. This must be differentiated from other causes of post vitrectomy glaucoma. Cataract extraction with posterior capsulotomy controls the condition. PMID:26056429

  4. SUR LE PARAMAGNTISME DU MANGANSE Par Mlle A. SERRES.

    E-print Network

    Paris-Sud XI, Université de

    présentaient même un faible ferromagnétisme. Depuis les travaux de Miss Gayler (4) on sait pré- parer du) a vérifié que -le manganèse très pur obtenu par Miss Gayler ne présentait aucune trace de ferro- magnétisme, A .13~., p. 266. 1 (8) SHIMIZU. Sc. Rep. Tohoku Imp. 1930,19, p. 411. (7) GRUBE et WINKLER. Z

  5. Filtrage de bioprocede de depollution. Approche par convolution particulaire.

    E-print Network

    Rossi, Vivien

    ´erobie mod´elis´ee par une approche bilan-mati`ere. A. Mod´elisation de la bior´eaction Ce mod`ele est de´etis´ee, selon le sch´ema d'Eu- ler, de ce mod`ele d'EDO. B. Description des variables Toutes les variables d

  6. INSTITUT POLYTECHNIQUE DE GRENOBLE attribue par la biblioth`eque

    E-print Network

    Paris-Sud XI, Université de

    , pour son agr´eable compagnie et les courses `a pied qu'on a partag´ees. Mes co-bureaux, Jordi et Pepa, pour leur amiti´e et tous les discussions et repas par- tag´es. Nicolas, Julien, pour... le Roy Nock

  7. LES MOLCULES DANS UN CHAMP CENTRIFUGE INTENSE; par T. SVEDBERG.

    E-print Network

    Paris-Sud XI, Université de

    LES MOLÉCULES DANS UN CHAMP CENTRIFUGE INTENSE; par T. SVEDBERG. Université d'Upsala (Suède). Sommaire. 2014 De l'étude du comportement des molécules dans des champs centrifuges intenses on peut tirer exposée. L'auteur décrit la réalisation des champs centrifuges allant jusqu'à 200 000 fois la pesanteur

  8. Scheduling on the MasPar SIMD parallel computer 

    E-print Network

    Perkins, Keith Douglas

    1995-01-01

    whose scheduling times are within 90% of optimal schedules 95% of the time. The highest level first algorithm was then modifies to handle the processor architecture on the Texas A&M University's MasPar parallel computer. These modifications included...

  9. Signaling crosstalk between TGF? and Dishevelled/Par1b.

    PubMed

    Mamidi, A; Inui, M; Manfrin, A; Soligo, S; Enzo, E; Aragona, M; Cordenonsi, M; Wessely, O; Dupont, S; Piccolo, S

    2012-10-01

    Crosstalk of signaling pathways is critical during metazoan development and adult tissue homeostasis. Even though the transforming growth factor-beta (TGF?) transduction cascade is rather simple, in vivo responsiveness to TGF? ligands is tightly regulated at several steps. As such, TGF? represents a paradigm for how the activity of one signaling system is modulated by others. Here, we report an unsuspected regulatory step involving Dishevelled (Dvl) and Par1b (also known as MARK2). Dvl and Par1b cooperate to enable TGF?/bone morphogenetic protein (BMP) signaling in Xenopus mesoderm development and TGF? responsiveness in mammalian cells. Mechanistically, the assembly of the Par1b/Dvl3/Smad4 complex is fostered by Wnt5a. The association of Smad4 to Dvl/Par1 prevents its inhibitory ubiquitination by ectodermin (also known as transcriptional intermediary factor 1 gamma or tripartite motif protein 33). We propose that this crosstalk is relevant to coordinate TGF? responses with Wnt-noncanonical and polarity pathways. PMID:22576663

  10. Filtrage par Noyaux de Convolution Iteratif Yuting Chen 1

    E-print Network

    Paris-Sud XI, Université de

    - ficult to apply. Nevertheless, Convolution Particle Filtering (CPF) was shown to be an efficient approach to all kinds of dynamic models, and appears robust. Keywords. Hidden Markov models, Nonlinear filters´ethode est par ailleurs g´en´erique, robuste et facilement adaptable. Mots-cl´es. Mod`ele de Markov cach

  11. INSTITUT NATIONAL POLYTECHNIQUE DE GRENOBLE N attribu par la bibliothque

    E-print Network

    Paris-Sud XI, Université de

    , pour le suivi de mon travail, ses conseils au cours de ces trois années et ses suggestions pour'Antony, qui a encadré ce travail de recherche. Il a su par ses idées, ses conseils, ses explications et ses et de la clairvoyance de ses opinions. La confiance qu'il m'a accordée et son soutien constant ont

  12. The pars intermedia of the mink, Mustela vison

    Microsoft Academic Search

    B. Weman; A. Nobin

    1973-01-01

    The pars intermedia of intact and experimental female minks has been studied by light, electron and fluorescence microscopy. The general structure of the mink intermediate lobe is described. Two main cell types are recognized. One, termed glandular cell, predominates in number and is characterized by the presence of electron-dense granules about 200 nm in diameter and numerous large vesicles up

  13. Proposition de thse Contribution la modlisation physique du dosage par

    E-print Network

    Demouchy, Sylvie

    Proposition de thèse Contribution à la modélisation physique du dosage par microanalyse'étude proposée consiste à revoir les paramètres physiques fondamentaux intégrés dans les modèles analytiques et physique analytique mais son aboutissement est très appliqué car il permettra de quantifier avec précision

  14. Cheminement par trimestre -Baccalaurat en informatique La passerelle DEC SIM

    E-print Network

    Montréal, Université de

    Cheminement par trimestre - Baccalauréat en informatique La passerelle DEC SIM Le baccalauréat en informatique Le baccalauréat en informatique mise sur les aspects fondamentaux de l'informatique, favorisant l en mathématiques appliquées à l'informatique et à l'optimisation. On y aborde les problèmes pratiques

  15. Cheminement par trimestre --Mineure en informatique La mineure en informatique

    E-print Network

    Montréal, Université de

    Cheminement par trimestre -- Mineure en informatique La mineure en informatique La mineure est informatique. Ce programme est conçu pour être suivi concurremment avec une majeure ou d'autres mineures, et il cheminement d'une mineure en informatique peut être complété en 3 trimestres, avec des études à temps plein

  16. Cheminement par trimestre --Baccalaurat en informatique La passerelle DEC informatique

    E-print Network

    Montréal, Université de

    Cheminement par trimestre -- Baccalauréat en informatique La passerelle DEC informatique Le baccalauréat en informatique Le baccalauréat en informatique mise sur les aspects fondamentaux de l'informatique program- mation et en mathématiques appliquées à l'informatique et à l'optimisation. On y aborde les

  17. Cheminement par trimestre Le Baccalaurat en mathmatiques et informatique

    E-print Network

    Montréal, Université de

    Cheminement par trimestre Le Baccalauréat en mathématiques et informatique Le baccalauréat Le baccalauréat en mathématiques et informatique assure une formation solide en mathéma- tiques tout en permettant l'accès à un bon nombre de cours d'informatique offerts au pro- gramme. Plusieurs cours de

  18. Cheminement par trimestre --Majeure en informatique La majeure en informatique

    E-print Network

    Montréal, Université de

    Cheminement par trimestre -- Majeure en informatique La majeure en informatique La majeure est destinée à ceux qui désirent acquérir une formation en informatique ainsi qu'une for- mation de base dans une autre discipline. Elle permet l'accès à presque tous les cours d'informatique offerts au

  19. Acetylcholinesterase in der Pars distalis von Zonotrichia leucophrys gambelii (Aves)

    Microsoft Academic Search

    Eberhard Haase; Donald S. Farner

    1969-01-01

    Photoperiodic stimulation of photosensitive male white-crowned sparrows, Zonotrichia leucophrys gambelii, causes the appearance of acetylcholinesterase activity in the cephalic and caudal lobes of the pars distalis. In photosensitive birds held on short daily photoperiods little or no activity can he demonstrated histochemically. The enzyme is restricted to PAS-positive cells. After attaining a maximum between 31 and 52 days after the

  20. The Nucleus mesencephalicus lateralis, pars dorsalis in Neotropical Passerines

    Microsoft Academic Search

    Michael K. Rylander

    1979-01-01

    The volume of the nucleus mesencephalicus lateralis, pars dorsalis and the optic lobe were determined in 8 species of neotropical birds in the order Passeriformes: Pyrocephalus rubinus, Pitangus sulphuratus, Atticora fasciata, Molothrus bonariensis, Tangara nigrocincta, Ramphocelus dimidiatus, Euphonia xanthogaster and Spinus magellanicus. The ratio of the volume of the nucleus to the volume of the optic lobe was calculated for

  1. APPLICATION DES PALIERS A GAZ AUX GYROSCOPES Par G. GOBERT,

    E-print Network

    Paris-Sud XI, Université de

    43 APPLICATION DES PALIERS A GAZ AUX GYROSCOPES Par G. GOBERT, Centre d'Études Nucléaires de Saclay gyroscope dont le rotor de 4 cm de diamètre pesant plusieurs centaines de grammes tourne à une vitesse to the construction of a gyroscope. The rotor is 4 cm in diameter and runs at 180 000 r. p. m. Its weight is 500 g. LE

  2. UTILISATION MTABOLIQUE DES ACIDES GRAS DU TISSU ADIPEUX PAR LA POULE EN PONTE

    E-print Network

    Paris-Sud XI, Université de

    UTILISATION MÉTABOLIQUE DES ACIDES GRAS DU TISSU ADIPEUX PAR LA POULE EN PONTE B. LECLERCQ Marie . Le tissu adipeux de huit poules pondeuses est marqué par l'acide palmitique-1-14C. On suit pendant z8 contribution des réserves pour la vitellogenèse varie beaucoup d'une poule à l'autre et ne peut s'expliquer par

  3. ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The small serine resolvase ParA from bacterial plasmids RK2 and RP4 catalyzes the recombination of two identical 133 bp recombination sites known as MRS. Previously, we reported that ParA is active in the fission yeast Schizosaccharomyces pombe. In this work, the parA recombinase gene was placed un...

  4. Recherche d'images par rgions d'intrt : segmentation grossire rapide

    E-print Network

    Fauqueur, Julien

    approximative car elle prend en compte toutes les parties d'images, y compris celles qui ne sont pas pertinentesRecherche d'images par régions d'intérêt : segmentation grossière rapide et description couleur.Fauqueur,Nozha.Boujemaa}@inria.fr RÉSUMÉ. Les deux problèmes posés par un système de recherche d'images par régions sont la définition et

  5. A New Method for PAR Reduction of OFDM Signal Based on Chaotic Encoder

    Microsoft Academic Search

    Yong Guo; Liang Huang

    2010-01-01

    In this paper, we propose using chaotic digital filter to encode the time domain signal xn to reduce peak-to-average power ratio (PAR) in OFDM system. The xn may have high PAR value, which is a main drawback of OFDM system. The time domain signal becomes chaotically distributed by chaotic encoding, so the PAR value can be reduced. Simulations are done

  6. TRANSLATIONAL PHYSIOLOGY Discharge Rate of Substantia Nigra Pars Reticulata Neurons Is Reduced In

    E-print Network

    Friedman, Nir

    TRANSLATIONAL PHYSIOLOGY Discharge Rate of Substantia Nigra Pars Reticulata Neurons Is Reduced. Discharge rate of substantia nigra pars reticulata neurons is reduced in non-parkinsonian monkeys in the activity of the substantia nigra pars reticulata (SNr) of non-parkinsonian (normal) monkeys

  7. Article original Quantits d'herbe ingres par les vaches laitires, les

    E-print Network

    Boyer, Edmond

    Article original Quantités d'herbe ingérées par les vaches laitières, les génisses et les moutons d'herbe de référence (dans le système français des unités d'encombrement) ingérées par des bovins de type très différent et les liaisons entre les quantités d'herbe ingérées par des ovins et des bovins

  8. Production maximale de matire sche et rayon-nement solaire intercept par un couvert vgtal

    E-print Network

    Paris-Sud XI, Université de

    ., 1982...) et a particulièrement mis en évidence l'importance de l'interception du rayonnement solaire absorbée par le couvert végétal est calculée à partir de Fi et du rayonnement solaire global G (PAR, = 0Production maximale de matière sèche et rayon- nement solaire intercepté par un couvert végétal

  9. Etudes par spectroscopie Raman et par RMN des verres du système B2O3SiO2Li2O

    Microsoft Academic Search

    Jean-Claude Brethous; Alain Levasseur; Gerard Villeneuve; Patrick Echegut; Paul Hagenmuller; Michel Couzi

    1981-01-01

    Une approche structurale par spectroscopie Raman des verres du système B2O3-SiO2-Li2O a été effectuée. D'autre part une étude de l'environment du bore par RMN a été entreprise. Les résultats obtenus semblent indiquer que ces verres sont constitués de deux phases vitreuses distinctes, respectivement boratée et silicatée. Des phénomènes de démixtion ont d'ailleurs été mis en évidence par microscopie électronique à

  10. PRODUCTION DE TRITIUM DANS LE THORIUM PAR DES PROTONS DE 135 MeV Par M. LEFORT, G. SIMONOFF et X. TARRAGO

    E-print Network

    Paris-Sud XI, Université de

    959 PRODUCTION DE TRITIUM DANS LE THORIUM PAR DES PROTONS DE 135 MeV Par M. LEFORT, G. SIMONOFF et Saclay. Résumé. 2014 On a mesuré la section efficace de production de tritium après bombardment de thorium par des protons de 135 MeV accélérés au synchro-cyclotron d'Orsay. Le tritium était extrait des

  11. Binding and Spreading of ParB on DNA Determine Its Biological Function in Pseudomonas aeruginosa?†

    PubMed Central

    Kusiak, Magdalena; Gapczy?ska, Anna; P?ochocka, Danuta; Thomas, Christopher M.; Jagura-Burdzy, Gra?yna

    2011-01-01

    ParB protein of Pseudomonas aeruginosa belongs to a widely represented ParB family of chromosomally and plasmid-encoded partitioning type IA proteins. Ten putative parS sites are dispersed in the P. aeruginosa chromosome, with eight of them localizing in the oriC domain. After binding to parS, ParB spreads on the DNA, causing transcriptional silencing of nearby genes (A. A. Bartosik et al., J. Bacteriol. 186:6983–6998, 2004). We have studied ParB derivatives impaired in spreading either due to loss of DNA-binding ability or oligomerization. We defined specific determinants outside of the helix-turn-helix motif responsible for DNA binding. Analysis confirmed the localization of the main dimerization domain in the C terminus of ParB but also mapped another self-interactive domain in the N-terminal domain. Reverse genetics were used to introduce five parB alleles impaired in spreading into the P. aeruginosa chromosome. The single amino acid substitutions in ParB causing a defect in oligomerization but not in DNA binding caused a chromosome segregation defect, slowed the growth rate, and impaired motilities, similarly to the pleiotropic phenotype of parB-null mutants, indicating that the ability to spread is vital for ParB function in the cell. The toxicity of ParB overproduction in Pseudomonas spp. is not due to the spreading since several ParB derivatives defective in oligomerization were still toxic for P. aeruginosa when provided in excess. PMID:21531806

  12. A New Class of Orthosteric uPAR·uPA Small-Molecule Antagonists Are Allosteric Inhibitors of the uPAR·Vitronectin Interaction.

    PubMed

    Liu, Degang; Zhou, Donghui; Wang, Bo; Knabe, William Eric; Meroueh, Samy O

    2015-06-19

    The urokinase receptor (uPAR) is a GPI-anchored cell surface receptor that is at the center of an intricate network of protein-protein interactions. Its immediate binding partners are the serine proteinase urokinase (uPA), and vitronectin (VTN), a component of the extracellular matrix. uPA and VTN bind at distinct sites on uPAR to promote extracellular matrix degradation and integrin signaling, respectively. Here, we report the discovery of a new class of pyrrolone small-molecule inhibitors of the tight ?1 nM uPAR·uPA protein-protein interaction. These compounds were designed to bind to the uPA pocket on uPAR. The highest affinity compound, namely 7, displaced a fluorescently labeled ?-helical peptide (AE147-FAM) with an inhibition constant Ki of 0.7 ?M and inhibited the tight uPAR·uPAATF interaction with an IC50 of 18 ?M. Biophysical studies with surface plasmon resonance showed that VTN binding is highly dependent on uPA. This cooperative binding was confirmed as 7, which binds at the uPAR·uPA interface, also inhibited the distal VTN·uPAR interaction. In cell culture, 7 blocked the uPAR·uPA interaction in uPAR-expressing human embryonic kidney (HEK-293) cells and impaired cell adhesion to VTN, a process that is mediated by integrins. As a result, 7 inhibited integrin signaling in MDA-MB-231 cancer cells as evidenced by a decrease in focal adhesion kinase (FAK) phosphorylation and Rac1 GTPase activation. Consistent with these results, 7 blocked breast MDA-MB-231 cancer cell invasion with IC50 values similar to those observed in ELISA and surface plasmon resonance competition studies. Explicit-solvent molecular dynamics simulations show that the cooperativity between uPA and VTN is attributed to stabilization of uPAR motion by uPA. In addition, free energy calculations revealed that uPA stabilizes the VTNSMB·uPAR interaction through more favorable electrostatics and entropy. Disruption of the uPAR·VTNSMB interaction by 7 is consistent with the cooperative binding to uPAR by uPA and VTN. Interestingly, the VTNSMB·uPAR interaction was less favorable in the VTNSMB·uPAR·7 complex suggesting potential cooperativity between 7 and VTN. Compound 7 provides an excellent starting point for the development of more potent derivatives to explore uPAR biology. PMID:25671694

  13. Brulure par Plaque de Bistouri Electrique: a Propos de Quatre Cas

    PubMed Central

    Khales, A.; Achbouk, A.; Belmir, R.; Cherkab, L.; Ennouhi, M.A.; Ababou, K.; Ihrai, H.

    2010-01-01

    Summary La brûlure par plaque de bistouri électrique est un accident rare mais grave par la profondeur de la lésion et par sa localisation, surtout quand qu’elle survient dans un contexte chirurgical dont le vécu reste difficile de la part du malade et du chirurgien. Cette brûlure bien que imprévisible reste grave par la profondeur et la localisation de la brûlure et par sa survenue dans un contexte opératoire, chez des patients malades. La prise en charge de la brûlure doit se faire en milieu spécialisé. La prévention reste le seul moyen d’éviter ce type d’accident. PMID:21991216

  14. Is PAR a Good Investment? Understanding the Costs and Benefits of Teacher Peer Assistance and Review Programs

    ERIC Educational Resources Information Center

    Papay, John P.; Johnson, Susan Moore

    2012-01-01

    Peer Assistance and Review (PAR) is a local labor-management initiative designed to improve teacher quality. In PAR, expert "consulting teachers" mentor, support, and evaluate novice and underperforming veteran teachers. Evaluations under PAR can lead to dismissals. The authors examine the costs and benefits of PAR, both financial and…

  15. REDUCTION DE L'OCTOOXYDE D'URANIUM EN DIOXYDE D'URANIUM PAR L'AMMONIAC

    E-print Network

    Paris-Sud XI, Université de

    REDUCTION DE L'OCTOOXYDE D'URANIUM EN DIOXYDE D'URANIUM PAR L'AMMONIAC P. Suhubiette1 , F'étude cinétique de la réduction de l'octooxyde d'uranium par l'ammoniac en dioxyde d'uranium. Cette réduction de l'octooxyde d'uranium par l'ammoniac, suivie par thermogravimétrie. L'équation stoechiométrique de

  16. Minimally invasive pars approach for foraminal disc herniation.

    PubMed

    Hitchon, Patrick W; Awe, Olatilewa O; Close, Liesl; Sukkarieh, Hamdi G

    2015-07-01

    We present a retrospective cohort study on the outcome of patients with foraminal disc herniations (FDH) treated with partial pars resections using minimally invasive surgery (MIS) through a tube. FDH present a challenge due to the more lateral trajectory needed for their excision and thereby more muscle dissection and possibly facet resection. Forty patients, 19 women and 21 men with a mean age±standard deviation of 58±12years, underwent MIS for FDH. Data on length of hospital stay, body mass index and outcomes were collected prospectively and reviewed retrospectively. Average length of stay following surgery was 1±1days. There was one wound infection and four unsatisfactory results. Using the Macnab score, good to excellent results were achieved in 89% of the patients. Based on these results, MIS surgery with partial pars resection is an attractive option in the treatment of FDH with low morbidity and short hospital stays. PMID:25882259

  17. Pancréatite surinfectée révélé par un abcès du psoas

    PubMed Central

    Mahamadoun, Coulibaly; Nawfal, Houari; Brahim, Boukatta; Sbai, Hicham; Nabil, Kanjaa

    2014-01-01

    Les pancréatites aigues grave peuvent se compliquer de pseudo kystes qui peuvent conduire à la formation de fistules, ces fistules peuvent être dirigées vers différentes régions avec différentes manifestations cliniques. Ces manifestations extra pancréatiques de la pancréatite aigue constituent, par leurs particularités cliniques, biologiques et radiologiques, un réel apport au diagnostic positif. Le pronostic est celui de la pancréatite et dépend du site de la fistulisation. Le traitement par laparotomie a longtemps été le «Gold Standard», ces dernières années ont vu le développement de moyens moins invasifs et donc offrants un minimum de morbi-mortalité (Chirurgie mini invasive, drainage percutané). Nous rapportons l'observation d'un patient ayant séjourné dans notre structure. PMID:25995783

  18. Matriser le temps de calcul par la logique linaire lgre

    E-print Network

    Powell, Geoffrey

    7 LIPN Maîtriser le temps de calcul par la logique linéaire légère Certains programmes tournent en'y a pas de séparation nette entre ceux qui ont un temps de traitement hors de proportion avec la taille des données et ceux qui admettent des bornes. Un énoncé du type « ces programmes terminent en un temps

  19. Matriaux par chimie douce la lumire du synchrotron

    E-print Network

    ;Diatomées glass Le verre #12;0 Si(OH)4 SiO(OH)3- SiO2(OH)22- 6 8 10 12 pH 20 40 60 80 100% précipitation de la silice à pH basiques H+ Na2O.SiO2 Eau Le verre par Chimie Douce Si HO HO OH OH Acide faible Base

  20. Dispositifs opto-électropiques à commande par coordonnées

    NASA Astrophysics Data System (ADS)

    Smovge, A. K.

    1994-06-01

    The approach has been explained theoretically and experimentally considering analog coordinate-controlled optoelectronic devices as an alternative to custom potentiometers and variable resistors, whose potentially unreliable element, a wiping electromechanical contact, is eliminated through the use of an optoelectronic conversion principle. The new designs have been proposed and requirements for discrete-continuous photoresistor structure have been determined by using both simple numeric and analytical models for a realization of the coordinate dependence of the output voltage and internal resistance with predetermined accuracy. The new type of optoelectronic coordinate-controlled delay line of millisecond-range pulsed signals have been proposed as well as the design of two-dimensional sensor of displacements. The new method for upgradding the noise immunity, based on the use of the photoconductivity response time dependence from the illuminance have been proposed. Une direction de recherches a été justifiée théoriquement et expérimentalement. Cette étude considère les dipositifs opto-électroniques à commande par coordonnées comme une alternative aux potentiomètres classiques dont l'élément défaillant éventuel, à savoir le contact électrique mobile de frottement, est supprimé par l'emploi d'une méthode de conversion optoélectronique. La nouvelle construction a été proposée et les exigences concernant la structure photorésistance discret-continu ont été déterminées par l'emploi des deux méthodes de calcul numérique et analytique. De nouvelles structures du dispositif de retard des signaux impulsionnels dans la gamme des millisecondes avec une durée de retard réglable à commande par coordonnées aussi bien que du vidéocapteur positionnel bidimensionnel sont proposées. Une nouvelle méthode de l'augmentation de sensibilité de ce vidéocapteur aux obstacles optiques, basée sur la dépendance du temps de la photoréponse du niveau d'éclairage est proposée.

  1. Neural crest specification by noncanonical Wnt signaling and PAR-1

    PubMed Central

    Ossipova, Olga; Sokol, Sergei Y.

    2011-01-01

    Neural crest (NC) cells are multipotent progenitors that form at the neural plate border, undergo epithelial-mesenchymal transition and migrate to diverse locations in vertebrate embryos to give rise to many cell types. Multiple signaling factors, including Wnt proteins, operate during early embryonic development to induce the NC cell fate. Whereas the requirement for the Wnt/?-catenin pathway in NC specification has been well established, a similar role for Wnt proteins that do not stabilize ?-catenin has remained unclear. Our gain- and loss-of-function experiments implicate Wnt11-like proteins in NC specification in Xenopus embryos. In support of this conclusion, modulation of ?-catenin-independent signaling through Dishevelled and Ror2 causes predictable changes in premigratory NC. Morpholino-mediated depletion experiments suggest that Wnt11R, a Wnt protein that is expressed in neuroectoderm adjacent to the NC territory, is required for NC formation. Wnt11-like signals might specify NC by altering the localization and activity of the serine/threonine polarity kinase PAR-1 (also known as microtubule-associated regulatory kinase or MARK), which itself plays an essential role in NC formation. Consistent with this model, PAR-1 RNA rescues NC markers in embryos in which noncanonical Wnt signaling has been blocked. These experiments identify novel roles for Wnt11R and PAR-1 in NC specification and reveal an unexpected connection between morphogenesis and cell fate. PMID:22110058

  2. Le hakka, par Hilary Chappell et Laurent Sagart Le dialecte hakka est parl par quelque 35 millions de locuteurs, principalement dans le nord du

    E-print Network

    Paris-Sud XI, Université de

    Le hakka, par Hilary Chappell et Laurent Sagart Le «dialecte» hakka est parlé par quelque 35 peuplement hakka: la région de basses montagnes aux confins des provinces du Guangdong, Fujian et Jiangxi animosité a pris au 19ème siècle la forme d'une véritable guerre entre Hakkas et locuteurs du cantonais

  3. par courriel > taxe-apprentissage@upmc.fr par courrier > Universit Pierre et Marie Curie Direction gnrale de la formation et de l'insertion

    E-print Network

    À RENVOYER par courriel > taxe-apprentissage@upmc.fr par courrier > Université Pierre et Marie Curie ­ Direction générale de la formation et de l'insertion professionnelle Taxe d : Code postal : Ville : N° SIRET : Nom, prénom du responsable de la taxe : Fonction : Tél.: Courriel

  4. Rsultats obtenus chez la truite Arc-en-ciel concernant l'absorption du glycocolle par l'intestin moyen et par l'intestin

    E-print Network

    Paris-Sud XI, Université de

    Résultats obtenus chez la truite Arc-en-ciel concernant l'absorption du glycocolle par l'intestin moyen et par l'intestin postérieur : rôle du sodium dans les différences observées G. BOGÉ, A. RIGAL, G and distal intestinal absorption of glycocolle in trout. Role of sodium in the differences observed

  5. Targeting the chromosome partitioning protein ParA in tuberculosis drug discovery

    PubMed Central

    Nisa, Shahista; Blokpoel, Marian C. J.; Robertson, Brian D.; Tyndall, Joel D. A.; Lun, Shichun; Bishai, William R.; O'Toole, Ronan

    2010-01-01

    Objective To identify inhibitors of the essential chromosome partitioning protein ParA that are active against Mycobacterium tuberculosis. Methods Antisense expression of the parA orthologue MSMEG_6939 was induced on the Mycobacterium smegmatis background. Screening of synthetic chemical libraries was performed to identify compounds with higher anti-mycobacterial activity in the presence of parA antisense. Differentially active compounds were validated for specific inhibition of purified ParA protein from M. tuberculosis (Rv3918c). ParA inhibitors were then characterized for their activity towards M. tuberculosis in vitro. Results Under a number of culture conditions, parA antisense expression in M. smegmatis resulted in reduced growth. This effect on growth provided a basis for the detection of compounds that increased susceptibility to expression of parA antisense. Two compounds identified from library screening, phenoxybenzamine and octoclothepin, also inhibited the in vitro ATPase activity of ParA from M. tuberculosis. Structural in silico analyses predict that phenoxybenzamine and octoclothepin undergo interactions compatible with the active site of ParA. Octoclothepin exhibited significant bacteriostatic activity towards M. tuberculosis. Conclusions Our data support the use of whole-cell differential antisense screens for the discovery of inhibitors of specific anti-tubercular drug targets. Using this approach, we have identified an inhibitor of purified ParA and whole cells of M. tuberculosis. PMID:20810423

  6. PAR-1 contributes to the innate immune response during viral infection

    PubMed Central

    Antoniak, Silvio; Owens, A. Phillip; Baunacke, Martin; Williams, Julie C.; Lee, Rebecca D.; Weithäuser, Alice; Sheridan, Patricia A.; Malz, Ronny; Luyendyk, James P.; Esserman, Denise A.; Trejo, JoAnn; Kirchhofer, Daniel; Blaxall, Burns C.; Pawlinski, Rafal; Beck, Melinda A.; Rauch, Ursula; Mackman, Nigel

    2013-01-01

    Coagulation is a host defense system that limits the spread of pathogens. Coagulation proteases, such as thrombin, also activate cells by cleaving PARs. In this study, we analyzed the role of PAR-1 in coxsackievirus B3–induced (CVB3-induced) myocarditis and influenza A infection. CVB3-infected Par1–/– mice expressed reduced levels of IFN-? and CXCL10 during the early phase of infection compared with Par1+/+ mice that resulted in higher viral loads and cardiac injury at day 8 after infection. Inhibition of either tissue factor or thrombin in WT mice also significantly increased CVB3 levels in the heart and cardiac injury compared with controls. BM transplantation experiments demonstrated that PAR-1 in nonhematopoietic cells protected mice from CVB3 infection. Transgenic mice overexpressing PAR-1 in cardiomyocytes had reduced CVB3-induced myocarditis. We found that cooperative signaling between PAR-1 and TLR3 in mouse cardiac fibroblasts enhanced activation of p38 and induction of IFN-? and CXCL10 expression. Par1–/– mice also had decreased CXCL10 expression and increased viral levels in the lung after influenza A infection compared with Par1+/+ mice. Our results indicate that the tissue factor/thrombin/PAR-1 pathway enhances IFN-? expression and contributes to the innate immune response during single-stranded RNA viral infection. PMID:23391721

  7. The intracellular carboxyl tail of the PAR-2 receptor controls intracellular signaling and cell death.

    PubMed

    Zhu, Zhihui; Stricker, Rolf; Li, Rong yu; Zündorf, Gregor; Reiser, Georg

    2015-03-01

    The protease-activated receptors are a group of unique G protein-coupled receptors, including PAR-1, PAR-2, PAR-3 and PAR-4. PAR-2 is activated by multiple trypsin-like serine proteases, including trypsin, tryptase and coagulation proteases. The clusters of phosphorylation sites in the PAR-2 carboxyl tail are suggested to be important for the binding of adaptor proteins to initiate intracellular signaling to Ca(2+) and mitogen-activated protein kinases. To explore the functional role of PAR-2 carboxyl tail in controlling intracellular Ca(2+), ERK and AKT signaling, a series of truncated mutants containing different clusters of serines/threonines were generated and expressed in HEK293 cells. Firstly, we observed that lack of the complete C-terminus of PAR-2 in a mutated receptor gave a relatively low level of localization on the cell plasma membrane. Secondly, the shortened carboxyl tail containing 13 amino acids was sufficient for receptor internalization. Thirdly, the cells expressing truncation mutants showed deficits in their capacity to couple to intracellular Ca(2+) and ERK and AKT signaling upon trypsin challenge. In addition, HEK293 cells carrying different PAR-2 truncation mutants displayed decreased levels of cell survival after long-lasting trypsin stimulation. In summary, the PAR-2 carboxyl tail was found to control the receptor localization, internalization, intracellular Ca(2+) responses and signaling to ERK and AKT. The latter can be considered to be important for cell death control. PMID:25519044

  8. Where polarity meets fusion: role of Par6 in trophoblast differentiation during placental development and preeclampsia.

    PubMed

    Sivasubramaniyam, Tharini; Garcia, Julia; Tagliaferro, Andrea; Melland-Smith, Megan; Chauvin, Sarah; Post, Martin; Todros, Tullia; Caniggia, Isabella

    2013-03-01

    Trophoblast cell fusion is a prerequisite for proper human placental development. Herein we examined the contribution of Par6 (Partitioning defective protein 6), a key regulator of cell polarity, to trophoblast cell fusion in human placental development. During early placentation, Par6 localized to nuclei of cytotrophoblast cells but with advancing gestation Par6 shifted its localization to the cytoplasm and apical brush border of the syncytium. Exposure of primary isolated trophoblasts to 3% O(2) resulted in elevated Par6 expression, maintenance of tight junction marker ZO-1 at cell boundaries, and decreased fusogenic syncytin 1 expression compared with cells cultured at 20% O(2). Treatment of choriocarcinoma BeWo cells with forskolin, a known inducer of fusion, increased syncytin 1 expression but decreased that of Par6 and ZO-1. Par6 overexpression in the presence of forskolin maintained ZO-1 at cell boundaries while decreasing syncytin 1 levels. In contrast, silencing of Par6 disrupted ZO-1 localization at cell boundaries and altered the expression and distribution of acetylated ?-tubulin. Par6 expression was elevated in preeclamptic placentas relative to normotensive preterm controls and Par6 located to trophoblast cells expressing ZO-1. Together, our data indicate that Par6 negatively regulates trophoblast fusion via its roles on tight junctions and cytoskeleton dynamics and provide novel insight into the contribution of this polarity marker in altered trophoblast cell fusion typical of preeclampsia. PMID:23341197

  9. Protease activated receptor-1, PAR1, promotes placenta trophoblast invasion and beta-catenin stabilization.

    PubMed

    Grisaru-Granovsky, Sorina; Maoz, Myriam; Barzilay, Oded; Yin, Yong-Jun; Prus, Diana; Bar-Shavit, Rachel

    2009-03-01

    Despite extensive efforts toward elucidation of the molecular pathway controlling cytotrophoblast (CTB) invasion to the uterine decidua, it remains poorly defined. There are striking similarities between tumor cell invasion and cytotrophoblast implantation to the deciduas whereby the role of Protease Activated Receptors (PARs) and wnt signaling is well recognized. We examine here consequences of modulation of PAR1 and PAR2 expression and function on CTB invasion and beta-catenin stabilization. Toward this end, we utilized a model system of extravillous trophoblast (EVT) organ culture and various placenta cell lines (e.g., JAR and HTR-8/Svneo). Activation of PAR1 induces EVT invasion while hPar1-SiRNA and PAR1 antagonist SCH79797--effectively inhibited it. In parallel, the Wnt inhibitor Dickkopf-1 (Dkk1) similarly inhibited it. Nuclear localization of beta-catenin is seen only after PAR1 activation, and is markedly reduced following the application of hPar1-SiRNA construct and PAR1 antagonist in CTBs. In contrast, PAR2 elicited a low cytoplasmic beta-catenin level as also proliferation and invasion. In the non-activated CTBs in-comparison, beta-catenin appeared limited to the membrane pools. Concomitantly, a temporal regulated pattern of Wnt-4, 5a, 7b, 10a, 10b expression is seen along PAR1 appearance. Enforced expression of Wnt antagonists, Secreted Frizzled Related Proteins; SFRP2 & 5; into HTR-8/Svneo, resulted with a markedly reduced nuclear beta-catenin levels, similar to the effect obtained by hPar1-SiRNA treatment. Identification of PAR1 downstream target/s may nonetheless contribute to the formation of a future platform system for eliciting a firm placenta-uterus interactions and to the definition of late pregnancy outcomes. PMID:19040205

  10. Estimating Photosynthetically Available Radiation (PAR) at the Earth's surface from satellite observations

    NASA Technical Reports Server (NTRS)

    Frouin, Robert

    1993-01-01

    Current satellite algorithms to estimate photosynthetically available radiation (PAR) at the earth' s surface are reviewed. PAR is deduced either from an insolation estimate or obtained directly from top-of-atmosphere solar radiances. The characteristics of both approaches are contrasted and typical results are presented. The inaccuracies reported, about 10 percent and 6 percent on daily and monthly time scales, respectively, are useful to model oceanic and terrestrial primary productivity. At those time scales variability due to clouds in the ratio of PAR and insolation is reduced, making it possible to deduce PAR directly from insolation climatologies (satellite or other) that are currently available or being produced. Improvements, however, are needed in conditions of broken cloudiness and over ice/snow. If not addressed properly, calibration/validation issues may prevent quantitative use of the PAR estimates in studies of climatic change. The prospects are good for an accurate, long-term climatology of PAR over the globe.

  11. Regulation of microtubule stability and organization by mammalian Par3 in specifying neuronal polarity

    PubMed Central

    Chen, She; Chen, Jia; Shi, Hang; Wei, Michelle; Castaneda-Castellanos, David R.; Bultje, Ronald S.; Pei, Xin; Kriegstein, Arnold R.; Zhang, Mingjie; Shi, Song-Hai

    2012-01-01

    SUMMARY Polarization of mammalian neurons with a specified axon requires precise regulation of microtubule and actin dynamics in the developing neurites. Here we show that mammalian partition defective 3 (mPar3), a key component of the Par polarity complex that regulates the polarization of many cell types including neurons, directly regulates microtubule stability and organization. The N-terminal portion of mPar3 exhibits strong microtubule binding, bundling and stabilization activity, which can be suppressed by its C-terminal portion via an intra-molecular interaction. Interestingly, the inter-molecular oligomerization of mPar3 is able to relieve the intra-molecular interaction and thereby promote microtubule bundling and stabilization. Furthermore, disruption of this microtubule regulatory activity of mPar3 impairs its function in axon specification. Together, these results demonstrate a role for mPar3 in directly regulating microtubule organization that is crucial for neuronal polarization. PMID:23273878

  12. Paracrine Apoptotic Effect of p53 Mediated by Tumor Suppressor Par-4

    PubMed Central

    Burikhanov, Ravshan; Shrestha-Bhattarai, Tripti; Hebbar, Nikhil; Qiu, Shirley; Zhao, Yanming; Zambetti, Gerard P.; Rangnekar, Vivek M.

    2014-01-01

    Summary The guardian of the genome, p53, is often mutated in cancer and may contribute to therapeutic resistance. As p53 is intact and functional in normal tissues, we harnessed its potential to inhibit the growth of p53-deficient cancer cells. Specific activation of p53 in normal fibroblasts selectively induced apoptosis in p53-deficient cancer cells. This paracrine effect was mediated by p53-dependent secretion of the tumor suppressor Par-4. Accordingly, activation of p53 in normal mice, but not in p53?/? or Par-4?/? mice, caused systemic elevation of Par-4 that induced apoptosis of p53-deficient tumor cells. Mechanistically, p53 induced Par-4 secretion by suppressing the expression of UACA, which sequesters Par-4. Thus, normal cells can be empowered by p53 activation to induce Par-4 secretion for inhibition of therapy-resistant tumors. PMID:24412360

  13. 1 Cours de M.RUMIN rcrit par J.KULCSAR Chapitre VIII

    E-print Network

    Rumin, Michel

    1 Cours de M.RUMIN réécrit par J.KULCSAR Chapitre VIII Calcul matriciel Dans ce cours, désigne , ou coordonnées de chaque ( ) en colonne dans la matrice. #12;2 Cours de M.RUMIN réécrit par J.KULCSAR Définition. ( )( ) ( ) ( ) ( ) #12;3 Cours de M.RUMIN réécrit par J.KULCSAR On sait que ( ) est une base canonique de [ ] On a donc

  14. 1 Cours de M.RUMIN rcrit par J.KULCSAR Chapitre III

    E-print Network

    Rumin, Michel

    1 Cours de M.RUMIN réécrit par J.KULCSAR Chapitre III Espaces vectoriels Dans ce cours, le symbole sur la droite , le plan et l'espace . #12;2 Cours de M.RUMIN réécrit par J.KULCSAR B - Les espaces;3 Cours de M.RUMIN réécrit par J.KULCSAR Dans le plan , il y a essentiellement 2 façons simples de définir

  15. Ion transport induced by proteinase-activated receptors (PAR2) in colon and airways

    Microsoft Academic Search

    Karl Kunzelmann; Rainer Schreiber; jens König; Marcus Mall

    2002-01-01

    Protease-activated receptors type 2 (PAR2) are activated by serine proteases like trypsin and mast cell tryptase. The function\\u000a and physiological significance of PAR2 receptors is poorly understood, but recent studies suggest a role during inflammatory\\u000a processes in both airways and intestine. PAR2 receptors are also likely to participate in the control of ion transport in\\u000a these tissues. We demonstrate that

  16. A low-complexity PAR-reduction method for DMT-VDSL

    Microsoft Academic Search

    Per Ola B Orjesson; Hans G. Feichtinger; Niklas Grip; Mikael Isaksson; Norbert Kaiblinger; Per Odling; Lars-erik Persson

    Abstract DMT-VDSL signals have a high peak-to-average power ratio (PAR). In the transmitters, the PAR governs the necessary resolution of the digital-to-analog converter (DAC) and is an important factor for the power consumption of the line-driver. Aiming at implementation in a specific system, we propose a low complexity PAR-reduction method based on the iterative algorithm derived in [5, 20, 24,

  17. ScalParC: A New Scalable and Efficient Parallel Classification Algorithm for Mining Large Datasets

    Microsoft Academic Search

    Mahesh V. Joshi; George Karypis; Vipin Kumar

    1998-01-01

    In this paper, we present ScalParC ( Scalable Parallel Classifier), a new parallel formulation of a decision tree based classification process. Like other state-of-the-art de- cision tree classifiers such as SPRINT, ScalParC is suited for handling large datasets. We show that existing parallel formulation of SPRINT is unscalable, whereas ScalParC is shown to be scalable in both runtime and memory

  18. Biased signalling and proteinase-activated receptors (PARs): targeting inflammatory disease

    PubMed Central

    Hollenberg, M D; Mihara, K; Polley, D; Suen, J Y; Han, A; Fairlie, D P; Ramachandran, R

    2014-01-01

    Although it has been known since the 1960s that trypsin and chymotrypsin can mimic hormone action in tissues, it took until the 1990s to discover that serine proteinases can regulate cells by cleaving and activating a unique four-member family of GPCRs known as proteinase-activated receptors (PARs). PAR activation involves the proteolytic exposure of its N-terminal receptor sequence that folds back to function as a ‘tethered’ receptor-activating ligand (TL). A key N-terminal arginine in each of PARs 1 to 4 has been singled out as a target for cleavage by thrombin (PARs 1, 3 and 4), trypsin (PARs 2 and 4) or other proteases to unmask the TL that activates signalling via Gq, Gi or G12/13. Similarly, synthetic receptor-activating peptides, corresponding to the exposed ‘TL sequences’ (e.g. SFLLRN—, for PAR1 or SLIGRL— for PAR2) can, like proteinase activation, also drive signalling via Gq, Gi and G12/13, without requiring receptor cleavage. Recent data show, however, that distinct proteinase-revealed ‘non-canonical’ PAR tethered-ligand sequences and PAR-activating agonist and antagonist peptide analogues can induce ‘biased’ PAR signalling, for example, via G12/13-MAPKinase instead of Gq-calcium. This overview summarizes implications of this ‘biased’ signalling by PAR agonists and antagonists for the recognized roles the PARs play in inflammatory settings. Linked ArticlesThis article is part of a themed section on Molecular Pharmacology of GPCRs. To view the other articles in this section visit http://dx.doi.org/10.1111/bph.2014.171.issue-5 PMID:24354792

  19. Ionisation totale de diffrents gaz par les rayons 03B1 de l'uranium1

    E-print Network

    Paris-Sud XI, Université de

    Ionisation totale de différents gaz par les rayons 03B1 de l'uranium1 Par H. LABY, [Laboratoire de par Rutherfbrd 2. Avec une sphère de laiton de 1,1t centimètre de rayon recouverte d'oxyde d'uranium de l'hydrogène. Méthodes Expérimentales. Comme source de rayons x, on a choisi l'oxyde d'uranium U3

  20. Subpopulations of uPAR+ contribute to vasculogenic mimicry and metastasis in large cell lung cancer.

    PubMed

    Li, Yanlei; Sun, Baocun; Zhao, Xiulan; Zhang, Danfang; Wang, Xudong; Zhu, Dongwang; Yang, Zhihong; Qiu, Zhiqiang; Ban, Xinchao

    2015-04-01

    The urokinase plasminogen activator receptor (uPAR) is closely associated with poor prognosis in various aggressive cancers including large-cell lung cancer (LCLC). Vasculogenic mimicry (VM) refers to the unique capability of aggressive tumor cells to mimic the pattern of embryonic vasculogenic networks involving the blood supply in early tumor formation. We demonstrate the statistically positive correlation of uPAR expression with VM formation, metastasis, and poor prognosis of LCLC patients. uPAR(+) cells sorted from the LCLC H460 cell line show higher invasion, migration capacity, and tube structure formation capability on Matrigel compared with uPAR(-) cells. uPAR(+) tumor cells highly expressed vimentin and VE-cadherin; the epithelial marker E-cadherin was low expressed. Higher EMT-regulated protein twist and snail expressions were also observed in these cells. uPAR(+) cells injected subcutaneously into nude mice markedly increased tumor growth, induced VM formation and liver metastasis; by contrast, uPAR(-) cells did not. The data suggest that uPAR expression may predict VM formation, tumor metastasis and poorer prognosis of LCLC patients. The uPAR gene may be used as a novel therapeutic target for inhibiting angiogenesis and metastasis in LCLC. PMID:25661888

  1. Activation of protease-activated receptors (PARs)-1 and -2 promotes alpha-smooth muscle actin expression and release of cytokines from human lung fibroblasts

    PubMed Central

    Asokananthan, Nithiananthan; Lan, Rommel S; Graham, Peter T; Bakker, Anthony J; Tokanovi?, Ana; Stewart, Geoffrey A

    2015-01-01

    Previous studies have shown that protease-activated receptors (PARs) play an important role in various physiological processes. In the present investigation, we determined the expression of PARs on human lung fibroblasts (HLF-1) and whether they were involved in cellular differentiation and pro-inflammatory cytokine and prostaglandin (PGE2) secretion. PAR-1, PAR-2, PAR-3, and PAR-4 were detected in fibroblasts using RT-PCR, immunocytochemistry, and flow cytometry. Increased expression of PAR-4, but not other PARs, was observed in fibroblasts stimulated with phorbol myristate acetate. The archetypical activators of PARs, namely, thrombin and trypsin, as well as PAR-1 and PAR-2 agonist peptides, stimulated transient increases in intracellular Ca2+, and promoted increased ?-smooth muscle actin expression. The proteolytic and peptidic PAR activators also stimulated the release of IL-6 and IL-8, as well as PGE2, with a rank order of potency of PAR-1 > PAR-2. The combined stimulation of PAR-1 and PAR-2 resulted in an additive release of both IL-6 and IL-8. In contrast, PAR-3 and PAR-4 agonist peptides, as well as all the PAR control peptides examined, were inactive. These results suggest an important role for PARs associated with fibroblasts in the modulation of inflammation and remodeling in the airway. PMID:25663523

  2. Cloning and characterization of the parC and parE genes of Streptococcus pneumoniae encoding DNA topoisomerase IV: role in fluoroquinolone resistance.

    PubMed Central

    Pan, X S; Fisher, L M

    1996-01-01

    DNA topoisomerase IV mediates chromosome segregation and is a potential target for antibacterial agents including new antipneumococcal fluoroquinolones. We have used hybridization to a Staphylococcus aureus gyrB probe in concert with chromosome walking to isolate the Streptococcus pneumoniae parE-parC locus, lying downstream of a putative new insertion sequence and encoding 647-residue ParE and 823-residue ParC subunits of DNA topoisomerase IV. These proteins exhibited greatest homology respectively to the GrlB (ParE) and GrlA (ParC) subunits of S. aureus DNA topoisomerase IV. When combined, whole-cell extracts of Escherichia coli strains expressing S. pneumoniae ParC or ParE proteins reconstituted a salt-insensitive ATP-dependent decatenase activity characteristic of DNA topoisomerase IV. A second gyrB homolog isolated from S. pneumoniae encoded a 648-residue protein which we identified as GyrB through its close homology both to counterparts in S. aureus and Bacillus subtilis and to the product of the S. pneumoniae nov-1 gene that confers novobiocin resistance. gyrB was not closely linked to gyrA. To examine the role of DNA topoisomerase IV in fluoroquinolone action and resistance in S. pneumoniae, we isolated mutant strains stepwise selected for resistance to increasing concentrations of ciprofloxacin. We analysed four low-level resistant mutants and showed that Ser-79 of ParC, equivalent to resistance hotspots Ser-80 of GrlA and Ser-84 of GyrA in S. aureus, was in each case substituted with Tyr. These results suggest that DNA topoisomerase IV is an important target for fluoroquinolones in S. pneumoniae and establish this organism as a useful gram-positive system for resistance studies. PMID:8763932

  3. A systematic computation scheme of PAR-WIG cruising performance

    NASA Astrophysics Data System (ADS)

    Ando, Shigenori

    1993-08-01

    A systematic computation scheme is presented for PAR-WIG cruising performance, on a FORTRAN program. It is suitable for implementation on PCs. Effects of many parameters on the transportation efficiency are explored. Two concepts are presented in three views and artist impressions. One is a smallest single-crewman vehicle for experiment, sports, or pleasure. The other is a large vehicle for civil transportation. Both have twin hulls, which are quite suitable for installing a 'SMALL-TAIL-WIG' or 'WIG-let' to establish longitudinal attitude stability.

  4. Inflammation and Macular Oedema after Pars Plana Vitrectomy

    PubMed Central

    Romano, Vito; Angi, Martina; del Grosso, Renata; Romano, Davide; Vinciguerra, Paolo; Romano, Mario R.

    2013-01-01

    Cystoid macular oedema (CMO) is a major cause of reduced vision following intraocular surgery. Although the aetiology of CMO is not completely clarified, intraocular inflammation is known to play a major role in its development. The macula may develop cytotoxic oedema when the primary lesion and fluid accumulation occur in the parenchymatous cells (intracellular oedema) or vasogenic oedema when the primary defect occurs in the blood-retinal barrier and leads to extracellular fluid accumulation (extracellular oedema). We report on the mechanisms of CMO formation after pars plana vitrectomy and associated surgical procedures and discuss possible therapeutic approaches. PMID:24288446

  5. The nucleus mesencephalicus lateralis, pars dorsalis in neotropical passerines.

    PubMed

    Rylander, M K

    1979-01-01

    The volume of the nucleus mesencephalicus lateralis, pars dorsalis and the optic lobe were determined in 8 species of neotropical birds in the order Passeriformes: Pyrocephalus rubinus, Pitangus sulphuratus, Atticora fasciata, Molothrus bonariensis, Tangara nigrocincta, Ramphocelus dimidiatus, Euphonia xanthogaster and Spinus magellanicus. The ratio of the volume of the nucleus to the volume of the optic lobe was calculated for each species, and was found to be larger in all 8 species than in a series of nonpasserine species examined previously by Cobb. A possible relationship between the relative size of this nucleus and the auditory capabilities of birds is discussed. PMID:526853

  6. Troubles psychotiques précipités par le mariage : étude de trois observations

    PubMed Central

    Jaweher, Masmoudi; Kammoun, Mohamed Faouzi; Inès, Feki; Imen, Baati; Rim, Sallami; Abdelaziz, Jaoua

    2013-01-01

    Le mariage est un évènement très investi dans notre culture arabo-musulmane. Il présente une situation à grande charge émotionnelle et ayant un vécu stressant. C'est ainsi qu'il peut être à l'origine de la décompensation de certains troubles psychiatriques. Ce moment particulier de déclenchement de la pathologie peut altérer significativement l'adaptation familiale et sociale du patient en question, le rendant dépendant en partie ou en totalité à une institution. Dans ce travail, nous proposons d’étudier certains facteurs psychiques, sociaux et culturels pouvant aboutir à la précipitation des manifestations psychotiques par le mariage. Il s'agit de l’étude de trois observations cliniques, deux hommes et une femme, hospitalisés dans le service de psychiatrie A du CHU Hédi Chaker de Sfax et qui ont développés des manifestations psychotiques de façon concomitante à leur mariage. La durée moyenne de survenue des crises a été de vingt ans, le diagnostic retenu a été celui de trouble bipolaire dans deux cas et d'une schizophrénie indifférenciée chez le troisième patient. L’évolution s'est faite vers une chronicisation de deux malades et une dépendance institutionnelle dans le troisième cas. La précipitation des troubles psychotiques par le mariage, reste un phénomène en relation intime avec les composantes culturelles, elles-mêmes sont déterminantes dans la prise en charge ultérieure de ces patients. PMID:23785551

  7. Regulation of Urokinase Receptor Expression by p53: Novel Role in Stabilization of uPAR mRNA?

    PubMed Central

    Shetty, Sreerama; Velusamy, Thirunavukkarasu; Idell, Steven; Shetty, Praveenkumar; Mazar, Andrew P.; Bhandary, Yashodhar P.; Shetty, Rashmi S.

    2007-01-01

    We found that p53-deficient (p53?/?) lung carcinoma (H1299) cells express robust levels of cell surface uPAR and uPAR mRNA. Expression of p53 protein in p53?/? cells suppressed basal and urokinase (uPA)-induced cell surface uPAR protein and increased uPAR mRNA degradation. Inhibition of p53 by RNA silencing in Beas2B human airway epithelial cells conversely increased basal as well as uPA-mediated uPAR expression and stabilized uPAR mRNA. Purified p53 protein specifically binds to the uPAR mRNA 3? untranslated region (3?UTR), and endogenous uPAR mRNA associates with p53. The p53 binding region involves a 37-nucleotide uPAR 3?UTR sequence, and insertion of the p53 binding sequence into ?-globin mRNA destabilized ?-globin mRNA. Inhibition of p53 expression in these cells reverses decay of chimeric ?-globin-uPAR mRNA. These observations demonstrate a novel regulatory role for p53 as a uPAR mRNA binding protein that down-regulates uPAR expression, destabilizes uPAR mRNA, and thereby contributes to the viability of human airway epithelial or lung carcinoma cells. PMID:17548471

  8. Regulation of urokinase receptor expression by p53: novel role in stabilization of uPAR mRNA.

    PubMed

    Shetty, Sreerama; Velusamy, Thirunavukkarasu; Idell, Steven; Shetty, Praveenkumar; Mazar, Andrew P; Bhandary, Yashodhar P; Shetty, Rashmi S

    2007-08-01

    We found that p53-deficient (p53(-/-)) lung carcinoma (H1299) cells express robust levels of cell surface uPAR and uPAR mRNA. Expression of p53 protein in p53(-/-) cells suppressed basal and urokinase (uPA)-induced cell surface uPAR protein and increased uPAR mRNA degradation. Inhibition of p53 by RNA silencing in Beas2B human airway epithelial cells conversely increased basal as well as uPA-mediated uPAR expression and stabilized uPAR mRNA. Purified p53 protein specifically binds to the uPAR mRNA 3' untranslated region (3'UTR), and endogenous uPAR mRNA associates with p53. The p53 binding region involves a 37-nucleotide uPAR 3'UTR sequence, and insertion of the p53 binding sequence into beta-globin mRNA destabilized beta-globin mRNA. Inhibition of p53 expression in these cells reverses decay of chimeric beta-globin-uPAR mRNA. These observations demonstrate a novel regulatory role for p53 as a uPAR mRNA binding protein that down-regulates uPAR expression, destabilizes uPAR mRNA, and thereby contributes to the viability of human airway epithelial or lung carcinoma cells. PMID:17548471

  9. Secreted uPAR isoform 2 (uPAR7b) is a novel direct target of miR-221.

    PubMed

    Falkenberg, Natalie; Anastasov, Nataša; Schaub, Annalisa; Radulovic, Vanja; Schmitt, Manfred; Magdolen, Viktor; Aubele, Michaela

    2015-04-10

    miR-221/-222 and components of the urokinase-type plasminogen activator system (uPAS) are associated with metastasis and poor prognosis in breast cancer, including the triple-negative subtype (TNBC). Modification of components of uPAS and involved miRNAs may contribute to targeted therapy for breast cancer patients. miR-221-/-222-overexpressing or miR-221-depleted cells were employed for qRT-PCR and Western blots to show associations of uPAR with miR-221/-222. To substantiate direct targeting of miR-221/-222 within 3' UTR of the uPAR isoform 2, in silico analysesand in vitro assays were conducted. Significant associations between miR-221 and uPAR isoform 2 expressions were observed at the mRNA and protein levels in breast cancer cells representing TNBC. For the first time, the uPAR isoform 2 was demonstrated as direct target for miR-221/-222. Inhibition of miR-221 reduced uPAR protein expression and expression of the tumor cell invasion markers vimentin and RHOC. These results demonstrate a direct and positive regulation of the secreted uPAR isoform 2 by miR-221, increasing its protein expression, a prerequisite for malignancy, while the other uPAR isoforms (1, 3 and 4) are indirectly regulated through miR-10b and miR-221/-222. By targeting uPAR isoforms and/or miRNA-221/-222, the diagnosis and therapy of breast cancer, in particular in TNBC, could be significantly improved. PMID:25797271

  10. TUDE AUX RAYONS X, PAR DIFFRACTION NEUTRONIQUE ET PAR EFFET MSSBAUER DES SOLUTIONS SOLIDES Ba(FexGa1-x)2O4

    E-print Network

    Paris-Sud XI, Université de

    509 ÉTUDE AUX RAYONS X, PAR DIFFRACTION NEUTRONIQUE ET PAR EFFET MÖSSBAUER DES SOLUTIONS SOLIDES Ba Ba(FexGa1-x)2O4. Comme pour l'étude de la solution solide Ba(FexAl1-x)2O4, nous avons mis en évidence diffraction and Mössbauer effect the solid solu- tion Ba(FexGa1-x)2O4. As in the study of the solid solution

  11. UTILISATION PAR LA POULE PONDEUSE DE L'HUILE D'ARACHIDE ISOMRISE

    E-print Network

    Paris-Sud XI, Université de

    UTILISATION PAR LA POULE PONDEUSE DE L'HUILE D'ARACHIDE ISOMÉRISÉE I. — SES EFFETS SUR LA Recherche agronomique SOMMAIRE Ingérés par la Poule pondeuse, les acides gras trans (huile d biosynthèse de l'acide stéarique. INTRODUCTION Dans un essai précédent, nous avions distribué à des poules

  12. Les prix de transfert pratiqus par les entreprises transnationales franaises et brsiliennes de 1994 2010

    E-print Network

    Paris-Sud XI, Université de

    Les prix de transfert pratiqués par les entreprises transnationales françaises et brésiliennes de Paulo- USP tel-00673607,version1-23Feb2012 #12;2 Les prix de transfert pratiqués par les entreprises: Les prix de transfert sont les prix auxquels les services, les biens corporels et les biens

  13. TUDE EXPRIMENTALE D'UN CONVERTISSEUR A PLASMA DE CSIUM Par E. DIEULESAINT et PH. DEFRANOULD,

    E-print Network

    Paris-Sud XI, Université de

    95 A. ÉTUDE EXPÉRIMENTALE D'UN CONVERTISSEUR A PLASMA DE CÉSIUM Par E. DIEULESAINT et PH fonctionnement et décrit quelques réalisations de convertisseurs à plasma de césium. Nous rapportons ici les résultats d'une suite d'essais effectués sur un de ces convertisseurs chauffés par bombardement électronique

  14. OBSERVATION DE PULSATIONS DANS LES FAISCEAUX ISSUS DE SOURCE H. F. Par T. GRJEBINE,

    E-print Network

    Boyer, Edmond

    remplacé une partie de l'attachement horizontal habituellement métal- lique par une canne en verre de façon - cence du verre. Il fallait toutefois obligatoirement mettre en évidence ce phénomène par cette méthode réalisé sur le Van de Graaff de 5 MeV. Le faisceau tombait sur un verre au plomb et le courant d'ions d

  15. SCELLEMENT TANCHE AU VIDE, DU VERRE ET DU MICA Par JACQUES LABEYRIE.

    E-print Network

    Paris-Sud XI, Université de

    20. SCELLEMENT ÉTANCHE AU VIDE, DU VERRE ET DU MICA Par JACQUES LABEYRIE. Commissariat à l'énergie atomique. Sommaire. - Les feuilles de mica, même minces (0,01mm), peuvent être scellées au verre par l 6tanche du mica au verre se fait habituellement au moyen de cires ou de résines, polymérisées ou non

  16. NOUVELLES TUDES SUR LES THERMOMTRES A GAZ; Par M. P. CHAPPUIS.

    E-print Network

    Boyer, Edmond

    mercure en verre dur et les thermomètres à azote, à acide carbonique et à hydrogène sous volume constant. L'échelle thermométrique du verre dur était représentée dans ces recherches par deux groupes de quatre thermomètres en verre dur, construits par M. Tonnelot à Paris, très soigneusement étudiés au

  17. TRANSPORT SUR VERRE ET TRAITEMENT DES MULSIONS NUCLAIRES SANS SUPPORT Par GEORGES KAYAS,

    E-print Network

    Paris-Sud XI, Université de

    621. TRANSPORT SUR VERRE ET TRAITEMENT DES ÉMULSIONS NUCLÉAIRES SANS SUPPORT Par GEORGES KAYAS verre des émulsions nucléaires sans support. LE JOURNAL DE PHYSIQUE ET LE RADIUM. TOME d4) NOVEMBRE 1953 émulsions sur verre spécialement traité au préalable, fourni par la maison Ilford. Ayant utilisé cette

  18. PROTECTION DES VERRES D'OPTIQUE ET MIROIRS ALUMINIS Par Mlle FLAMANT.

    E-print Network

    Paris-Sud XI, Université de

    PROTECTION DES VERRES D'OPTIQUE ET MIROIRS ALUMINIÉS Par Mlle FLAMANT. Summary.2014 The object faits pour essayer de protéger les verres très altérables et les miroirs aluminiés, des attaques ou corrosions provoquées par l'air extérieur, et surtout l'air marin. 1. Protection des verres. --- Nous avons

  19. UTILISATION DIGESTIVE ET RETENTION PAR LE PORC DU PHOSPHORE ET DU CALCIUM D'UN

    E-print Network

    Paris-Sud XI, Université de

    UTILISATION DIGESTIVE ET RETENTION PAR LE PORC DU PHOSPHORE ET DU CALCIUM D'UN PHOSPHATE NATUREL suivant : acétylgluconate, phosphate tricalcique, lactate, carbonate, phosphate d'éthyle et de calcium du calcium de ce phosphate par le porc en croissance. La mesure des quantités d'aliments ingérés et

  20. 1 Cours de M.RUMIN rcrit par J.KULCSAR Chapitre II

    E-print Network

    Rumin, Michel

    1 Cours de M.RUMIN réécrit par J.KULCSAR Chapitre II Notion de structure de groupe I ­ Définitions ( ). Proposition : est un sous-groupe de ( ) si et seulement si : - l'élément neutre , - , - , #12;2 Cours de M.RUMIN #12;3 Cours de M.RUMIN réécrit par J.KULCSAR - ( ) ( ) ( ) sont des corps plus connus. 2. Exemple

  1. Sur le parcours des rayons 03B1 de l'uranium Par Lon BLOCH,

    E-print Network

    Boyer, Edmond

    Sur le parcours des rayons 03B1 de l'uranium Par Léon BLOCH, Agrégé de l'Université de Paris parcours des rayons x de l'uranium a été dé- terminé par Bragg1 au moyen d'une méthode indirecte. Bragg a mesuré l'ionisatiol totale due au rayonnement de l'uranium lorsque la substance est recouverte d

  2. NOUVELLES DONNES SUR LA TRANSPLANTATION DES ŒUFS, CHEZ LA LAPINE, PAR VOIE VAGINALE

    E-print Network

    Paris-Sud XI, Université de

    de 89 ceufs sur 191 ocufs transplantés par perforation de la paroi vaginale (!6,6 p. 100), de 75 ceufs sur 444 œufs transplantés sans perforation de la paroi vaginale (x7 p. xoo) et de x3 sur 141 laparatomie de deux façons différentes : - Soit par perforation de la paroi vaginale : la pointe de la

  3. Questioning Our Questions: Assessing Question Asking Practices to Evaluate a yPAR Program

    ERIC Educational Resources Information Center

    Grace, Sarah; Langhout, Regina Day

    2014-01-01

    The purpose of this research was to examine question asking practices in a youth participatory action research (yPAR) after school program housed at an elementary school. The research question was: In which ways did the adult question asking practices in a yPAR setting challenge and/or reproduce conventional models of power in educational…

  4. Modeling the Establishment of PAR Protein Polarity in the One-Cell C. elegans Embryo

    E-print Network

    Howard, Martin

    Modeling the Establishment of PAR Protein Polarity in the One-Cell C. elegans Embryo Filipe ABSTRACT At the one-cell stage, the C. elegans embryo becomes polarized along the anterior-posterior axis by cytoskeletal rearrangement. Initially, the PAR proteins are uniformly distributed throughout the embryo. After

  5. UNIVERSIT D'ANGERS Anne 2007 IRM CELLULAIRE DE LYMPHOCYTES MARQUES PAR

    E-print Network

    Paris-Sud XI, Université de

    UNIVERSITÉ D'ANGERS Année 2007 N° 827 IRM CELLULAIRE DE LYMPHOCYTES MARQUES PAR DES NANOPARTICULES médicale ÉCOLE DOCTORALE D'ANGERS Présentée et soutenue publiquement Le 6 juillet 2007 A Angers Par Pierre Mowat Devant le jury ci-dessous : Pr. Jean-Jacques Le Jeune (Directeur de thèse), PU-PH Université d'Angers

  6. SUR L'ANALYSE THERMODYNAMIQUE DES FORCES LECTROMOTRICES. Par M. JEAN VILLEY.

    E-print Network

    Paris-Sud XI, Université de

    SUR L'ANALYSE THERMODYNAMIQUE DES FORCES ÉLECTROMOTRICES. Par M. JEAN VILLEY. Sommaire. 2014 L'auteur éclaire et complète; par l'analyse des diverses forces électro- motrices capables d'entretenir un courant permanent, deux remarques relatives au rôle des forces électroniques, qu'il avait publiées jadis à l

  7. 20. STRUCTURES TUNNEL DOPES PAR IMPLANTATION D'IONS Central Research Institute for Physics, Budapest. Hongrie

    E-print Network

    Paris-Sud XI, Université de

    comportement d'une structure p Ge-n GaAs est identique à celui d'une diode tunnel p-n GaAs. Le spectre de907 20. STRUCTURES TUNNEL DOPÉES PAR IMPLANTATION D'IONS F. MEZEI Central Research Institute diffusion électronique sur des impu- retés atomiques par effet tunnel implique pour prin- cipale difficulté

  8. STABILISATEURS PASSIFS DE TENSION OU DE COURANT EN PONT Par A. CARAVEL,

    E-print Network

    Paris-Sud XI, Université de

    187 STABILISATEURS PASSIFS DE TENSION OU DE COURANT EN PONT Par A. CARAVEL, Laboratoire d. On supprime cet effet résiduel en prenant la diode comme branche d'un pont de Wheatstone équilibré en régime on modifie l'autre. Il est possible de compenser cette variation résiduelle par un montage en pont ; la

  9. Evolution du complexe parasitaire constitu par Rhizoctonia cerealis Van der Hoeven et Pseudo-

    E-print Network

    Paris-Sud XI, Université de

    Evolution du complexe parasitaire constitué par Rhizoctonia cerealis Van der Hoeven et Pseudo causé par Rhizoctonia cerealis semble aujourd'hui prendre de l'extension. Des expérimentations sur blé composed of Rhizoctonia cerealis Van der Hoeven and Pseudocercosporella herpotrichoides (Fron) Deighton

  10. Dtection srologique des virus du court-nou de la vigne par le test ELISA.

    E-print Network

    Boyer, Edmond

    Détection sérologique des virus du court-noué de la vigne par le test ELISA. Bernard WALTER André mosaïque de l'arabis (AMV) et du « fanleaf» de la vigne (GFV) est réalisée par les méthodes ELISA sandwich by direct and indirect ELISA methods. The sensitivity and specificity of both methods are very similar

  11. LES FONDEMENTS DE L'AUDIT PAR LES BUSINESS RISKS : MOTIVATIONS

    E-print Network

    Paris-Sud XI, Université de

    1 LES FONDEMENTS DE L'AUDIT PAR LES « BUSINESS RISKS » : MOTIVATIONS PROFESSIONNELLES OU RAISONS'analyse de métier et des « Business Risks ». Les cabinets d'audit prétendent adopter la nouvelle approche par les « Business Risks », indépendance de l'auditeur, efficacité de l'audit. Abstract In recent

  12. Past Par Excellence Award Winners Presented by the Young Alumni Board

    E-print Network

    Provancher, William

    Past Par Excellence Award Winners Presented by the Young Alumni Board 1982 - 2010 2010 2009 Brad made 1994 Holly Sloan Smith, social worker and executive director of the Association of Junior League International, Inc., New York #12;Past Par Excellence Award Winners Page Presented by the Young Alumni Board

  13. Synthse de textures par transport optimal Julien RABIN1 et Gabriel PEYR2

    E-print Network

    Paris-Sud XI, Université de

    Synthèse de textures par transport optimal Julien RABIN1 et Gabriel PEYRÉ2 1CMLA, ENS de Cachan.peyre@ceremade.dauphine.fr Résumé ­ Cet article présente un formalisme générique pour la synthèse de textures couleurs par méthodes statistiques apprises sur une texture donnée en exemple. L'approche proposée généralise des méthodes classiques

  14. Paternal age related schizophrenia (PARS): latent subgroups detected by k-means clustering analysis

    E-print Network

    Ahn, Hongshik

    1 Paternal age related schizophrenia (PARS): latent subgroups detected by k-means clustering.antonius@nyumc.org #12;2 Abstract Background: Paternal age related schizophrenia (PARS) has been proposed as a subgroup of schizophrenia with distinct etiology, pathophysiology and symptoms. This study uses a k-means clustering

  15. Platelet Specific Promoters Are Insufficient to Express Protease Activated Receptor 1 (PAR1) Transgene in

    E-print Network

    Platelet Specific Promoters Are Insufficient to Express Protease Activated Receptor 1 (PAR1) Transgene in Mouse Platelets Amal Arachiche, Mari´a de la Fuente, Marvin T. Nieman* Department study of protease activated receptors (PARs) in platelets is complicated due to species specific

  16. RECHERCHES SUR LES NEUTRONS LENTS Par HANS VON HALBAN jun. et PIERRE PREISWERK.

    E-print Network

    Paris-Sud XI, Université de

    RECHERCHES SUR LES NEUTRONS LENTS Par HANS VON HALBAN jun. et PIERRE PREISWERK. Sommaire. 2014 Uoe série d'expériences sur les neutrons ralentis par la paraffine (neutrons lents) est discutée en liaison température, que le cadmium absorbe fortement les neutrons d'énergie thermique, mais qu'il est pratiquement

  17. Accs au texte intgral des articles Accs au texte intgral par le rsolveur de liens / 1

    E-print Network

    Maume-Deschamps, Véronique

    Accès au texte intégral des articles Accès au texte intégral par le résolveur de liens / 1 ­ Accès aux ressources Pour avoir accès aux ressources et au texte intégral des articles, vous devez articles 2 Accès au texte intégral par le résolveur de liens / 2 La BU est abonnée à la revue Accès au

  18. Analyse Statistique de la Pollution par les PM10 en Haute-Normandie

    E-print Network

    Boyer, Edmond

    and to quantify the effects of variables of different types, mainly meteorological versus other pollutantsAnalyse Statistique de la Pollution par les PM10 en Haute-Normandie Fran¸cois-Xavier Jolloisa travail porte sur l'analyse de la pollution par les particules PM10 en r´egion Haute- Normandie entre 2004

  19. La perception des changements comptables par les acteurs : le cas du Vit-Nam

    E-print Network

    Paris-Sud XI, Université de

    1 La perception des changements comptables par les acteurs : le cas du Viêt-Nam Nhu Tuyên Lê déterminants de la perception des changements comptables par les acteurs mêmes de la Comptabilité, dans le produit à cet effet, l'étude conduit à quatre résultats : (1) les perceptions des changements sont

  20. ABSORPTION INFRA-ROUGE DANS LES ALLIAGES NORMAUX Par B. CAROLI (1),

    E-print Network

    Boyer, Edmond

    884. ABSORPTION INFRA-ROUGE DANS LES ALLIAGES NORMAUX Par B. CAROLI (1), Résumé. - L'absorption de'électrons de Bloch, par une méthode quantique. On en déduit pour les fréquences faibles un coefficient d'absorption sont estimées sur un exemple simple. Abstract. 2014 Infra-red absorption by dilute alloys is studied

  1. Article original Relations entre le nombre de fœtus par corne

    E-print Network

    Paris-Sud XI, Université de

    Article original Relations entre le nombre de fœtus par corne utérine, la taille de portée à dans une corne utérine. Le nombre de foetus implantés était compté par endoscopie. Il existe une une corne sur le taux de survie foetale (-5,2 points) et le poids moyen des lapereaux à la naissance

  2. LA DTERMINATION DE LA CONSTANTE SOLAIRE PAR CLAUDE POUILLET JeanLouis Dufresne

    E-print Network

    a également déterminé l'absorption du rayonnement solaire par l'atmosphère. Le but de cet article est de la chaleur solaire, sur les pouvoirs rayonnants et absorbants de l'air atmosphérique, et sur la détermination de l'absorption du rayonnement solaire par l'atmosphère ; ­ la détermination de la constante

  3. Rfrigration solaire par simulation en absorption sche J. Flchon et F. Machizaud

    E-print Network

    Boyer, Edmond

    thermiques que lui imposerait le rayonnement solaire. Nous examinons ainsi la dissociation isotherme du rayonnement solaire par apport d'une énergie élec- trique convenable en mesurant le volume de NH3 909 Réfrigération solaire par simulation en absorption sèche J. Fléchon et F. Machizaud Laboratoire

  4. LA DTERMINATION DE LA CONSTANTE SOLAIRE PAR CLAUDE POUILLET JeanLouis Dufresne

    E-print Network

    Dufresne, Jean-Louis

    a également déterminé l'absorption du rayonnement solaire par l'atmosphère. Le but de cet article est de "Mémoire sur la chaleur solaire, sur les pouvoirs rayonnants et absorbants de l'air atmosphérique, et sur détermination de l'absorption du rayonnement solaire par l'atmosphère ; ­ la détermination de la constante

  5. Le patrimoine par les 5 sens Du 10 au 12 avril 2013

    E-print Network

    Brest, Université de

    que les animateurs et agents de valorisation de l'architecture et du patrimoine, les guidesLe patrimoine par les 5 sens Du 10 au 12 avril 2013 Soit 3 jours ­ 21 heures OBJECTIFS DE LA FORMATION L'objectif de cet enseignement est de mettre en valeur le potentiel pédagogique du patrimoine par

  6. De l'art du dtail militaire la gouvernementalit ... en passant par les disciplines

    E-print Network

    Boyer, Edmond

    dans : Michel Foucault, Savoirs, domination et sujet, Presses Universitaires de Rennes, 2008, pp. 121 pour prévenir le trouble civil ». Ce bref passage (rarement relevé) de MICHEL FOUCAULT dans SURVEILLER intrigué par le peu d'attention portée à cette valorisation de l'activité militaire par MICHEL FOUCAULT

  7. L'invite du mois Propos recueillis par Thierry Paquot (nov. 1995)

    E-print Network

    Boyer, Edmond

    L'invitée du mois Propos recueillis par Thierry Paquot (nov. 1995) Colette Petonnet Référence de publication : Colette Pétonnet, 1996 (janvier-février), « L'invitée du mois. Propos recueillis par Thierry autrement que "poétiquement" ? C'est avec cette injonction heideggerienne en tête que je rencontre Colette

  8. Q-Fit General Assessment Physical Activity Readiness Questionnaire (PAR-Q) Name ________________________________________________ Gender: ____M ____F

    E-print Network

    Azevedo, Ricardo

    medications that will affect your heart rate and/or blood pressure? If you answered NO to all questionsQ-Fit General Assessment Physical Activity Readiness Questionnaire (PAR-Q) Name:__________ For most people, physical activity should not pose any problem or hazard. PAR-Q has been designed

  9. HyParSAT: A Hybrid Parallel Complete SAT Solver Using

    E-print Network

    Kaminsky, Alan

    1 HyParSAT: A Hybrid Parallel Complete SAT Solver Using Parallel Java 2 By Jiten Patel Email: jrp Rochester Institute of Technology #12;2 ABSTRACT HyParSAT: HYBRID PARALLEL COMPLETE SAT SOLVER USING approach of a parallel complete SAT solver for shared memory multiprocessor (SMP) parallel computers

  10. Recherche dans les banques d'ADN par indexation parall`ele

    E-print Network

    Boyer, Edmond

    Recherche dans les banques d'ADN par indexation parall`ele Van Hoa Nguyen Institut Francophone d banques d'ADN. Une recherche rapide passe par une mise en oeuvre parall`ele des algorithmes. Les m directement sur le temps d'ex´ecution. Or les banques d'ADN croissent exponentiellement. Cet article propose

  11. Identification and characterization of yeasts causing chalk mould defects on par-baked bread

    Microsoft Academic Search

    N. Deschuyffeleer; K. Audenaert; S. Samapundo; S. Ameye; M. Eeckhout; F. Devlieghere

    2011-01-01

    Pichia anomala, Hyphopichia burtonii and Saccharomycopsis fibuligera are spoilage yeasts causing chalk mould defects on par-baked breads packaged under modified atmosphere. The first objective of this study was to identify yeasts isolated from spoiled par-baked breads by means of a RAPD protocol and to determine the dominant spoilers amongst identified strains. The second objective was to determine the effects of

  12. LISTE DES SPCIALITS PAR DOMAINE TRANSVERSAL, DISCIPLINE ET FACULT Bureau de la registraire -Section information et recrutement

    E-print Network

    Spino, Claude

    LISTE DES SPÉCIALITÉS PAR DOMAINE TRANSVERSAL, DISCIPLINE ET FACULTÉ Bureau de la registraire - Section information et recrutement Domaine transversal Discipline Faculté Domaine d'expertise et exemples Activités physiques et sportives EPS Supervison pédagogique; Responsabilisation par l'activité physique

  13. ANRS12154Pecan_NVP_v1_dernier enregistrement par Anne-Marie Population pharmacokinetic-pharmacogenetic study of1

    E-print Network

    Paris-Sud XI, Université de

    1 ANRS12154Pecan_NVP_v1_dernier enregistrement par Anne-Marie Population pharmacokinetic12154Pecan_NVP_v1_dernier enregistrement par Anne-Marie 1 Abstract2 The aims of this open-label, single;3 ANRS12154Pecan_NVP_v1_dernier enregistrement par Anne-Marie CYP2B6 516G>T genetic polymorphism

  14. Proteinase-activated receptors (PARs) – focus on receptor-receptor-interactions and their physiological and pathophysiological impact

    PubMed Central

    2013-01-01

    Proteinase-activated receptors (PARs) are a subfamily of G protein-coupled receptors (GPCRs) with four members, PAR1, PAR2, PAR3 and PAR4, playing critical functions in hemostasis, thrombosis, embryonic development, wound healing, inflammation and cancer progression. PARs are characterized by a unique activation mechanism involving receptor cleavage by different proteinases at specific sites within the extracellular amino-terminus and the exposure of amino-terminal “tethered ligand“ domains that bind to and activate the cleaved receptors. After activation, the PAR family members are able to stimulate complex intracellular signalling networks via classical G protein-mediated pathways and beta-arrestin signalling. In addition, different receptor crosstalk mechanisms critically contribute to a high diversity of PAR signal transduction and receptor-trafficking processes that result in multiple physiological effects. In this review, we summarize current information about PAR-initiated physical and functional receptor interactions and their physiological and pathological roles. We focus especially on PAR homo- and heterodimerization, transactivation of receptor tyrosine kinases (RTKs) and receptor serine/threonine kinases (RSTKs), communication with other GPCRs, toll-like receptors and NOD-like receptors, ion channel receptors, and on PAR association with cargo receptors. In addition, we discuss the suitability of these receptor interaction mechanisms as targets for modulating PAR signalling in disease. PMID:24215724

  15. Neuronal Activity in Substantia Nigra Pars Reticulata during Target Michele A. Basso1 and Robert H. Wurtz2

    E-print Network

    Kemnitz, Joseph

    Neuronal Activity in Substantia Nigra Pars Reticulata during Target Selection Michele A. Basso1 be identified altered the activity of substantia nigra pars reticulata (SNr) neurons of the basal ganglia (BG., 1992), and the substantia nigra pars reticulata (SNr), one of two output nuclei of the BG, has direct

  16. SPECTRES D'ABSORPTION K DU ZIRCONIUM, DE SON OXYDE ET DE SON OXYCHLORURE (*) Par ALEXIS GANSON,

    E-print Network

    Paris-Sud XI, Université de

    298. SPECTRES D'ABSORPTION K DU ZIRCONIUM, DE SON OXYDE ET DE SON OXYCHLORURE (*) Par ALEXIS GANSON'étude par spectroscopie X des spectres d'absorption K du zirconium, de ZrO2 et ZrOCl2 anhydres et hydratés mica courbé, par transmission. On montre que la discontinuité d'absorption K du zirconium métallique

  17. 396 LE LAIT / JUILLET-AOT 1975 / N 547 Contamination de Pont-l'Evque par un Penicillium

    E-print Network

    Boyer, Edmond

    396 LE LAIT / JUILLET-AOÛT 1975 / N° 547 par Contamination de Pont-l'Evêque par un Penicillium, les fromages Pont-l'Évêque d'une fromagerie de l'Orne furent envahis par une moisissure bleu-vert. Les développement mycélien. La culture nous fût soumise accompagnée d'un prélèvement direct des froma- ges de Pont

  18. LA DISPERSION ROTATOIRE MAGNTIQUE DE L'EAU DANS L'ULTRA-VIOLET Par M. G. BRUHAT.

    E-print Network

    Paris-Sud XI, Université de

    une série de mesures de la constante de Verdet de l'eau, faites par une méthode de polarisation/400 pour les raies des plus courtes longueurs d'onde. On voit que les constantes de Verdet données par les valeurs pBG des cons- tantes de Verdet trouvées par M. Guinier et moi; ces valeurs sont un peu

  19. [Development of agonists/antagonists for protease-activated receptors (PARs) and the possible therapeutic application to gastrointestinal diseases].

    PubMed

    Sekiguchi, Fumiko

    2005-06-01

    Protease-activated receptors (PARs), a family of G-protein-coupled seven-transmembrane-domain receptors, are activated by proteolytic unmasking of the N-terminal cryptic tethered ligand by certain serine proteases. Among four PAR family members cloned to date, PAR-1, PAR-2, and PAR-4 can also be activated through a non-enzymatic mechanism, which is achieved by direct binding of exogenously applied synthetic peptides based on the tethered ligand sequence, known as PARs-activating peptides, to the body of the receptor. Various peptide mimetics have been synthesized as agonists for PARs with improved potency, selectivity, and stability. Some peptide mimetics and/or nonpeptide compounds have also been developed as antagonists for PAR-1 and PAR-4. PARs are widely distributed in the mammalian body, especially throughout the alimentary systems, and play various roles in physiological/pathophysiological conditions, i.e., modulation of salivary, gastric, or pancreatic glandular exocrine secretion, gastrointestinal smooth muscle motility, gastric mucosal cytoprotection, suppression/facilitation of visceral pain and inflammation, etc. Thus PARs are now considered novel therapeutic targets, and development of selective agonists and/or antagonists for PARs might provide a novel strategy for the treatment of various diseases that are resistant to current therapeutics. PMID:15930817

  20. 400 LE LAIT / .JUILLET-AOUT 1969 / N 487 Etude de la coagulation du lait par la prsure

    E-print Network

    Paris-Sud XI, Université de

    400 LE LAIT / .JUILLET-AOUT 1969 / N° 487 Etude de la coagulation du lait par la présure et de la coagulation du lait par la présure a été abordée par des méthodes variées. Les plus simples et les plus été faites pour rendre plus rapide et automatique la mesure du temps de coagulation, notam- ment

  1. SUR LE PROBLME PLAN DE PROPAGATION POUR LE DOUBLET LECTRIQUE HORIZONTAL Par G. BOUDOURIS et D. ILIAS.

    E-print Network

    Paris-Sud XI, Université de

    A par un doublet éléctrique vertical place au point B.et ayant la longueur 1 et le moment pcos qJ. On se compo- sante &1,. On remarquera maintenant que le doublet pcos q ne doit pas produire au point B la composante &r rayonn6e par le doublet pcos cp ou, ce qui revient au meme, par le doublet p

  2. CERCLA interim action at the Par Pond unit: A case study

    SciTech Connect

    Hickey, H.M. [Westinghouse Savannah River Co., Aiken, SC (United States); Matthews, S.S.; Neal, L.W. [Rust Environment and Infrastructure, Inc., Greenville, SC (United States); Weiss, W.R. [Rust Environment and Infrastructure, Inc., Aiken, SC (United States)

    1993-11-01

    The Par Pond unit designated under CERCLA consists of sediments within a Savannah River Site (SRS) cooling water reservoir. The sediments are contaminated with radionuclides and nonradioactive constituents from nuclear production reactor operations. The mercury in Par Pond is believed to have originated from the Savannah River. Because of Par Pond Dam safety Issues, the water level of the reservoir was drawn down, exposing more than 1300 acres of contaminated sediments and triggering the need for CERCLA interim remedial action. This paper presents the interim action approach taken with Par Pond as a case study. The approach considered the complexity of the Par Pond ecosystem, the large size of Par Pond, the volume of contaminated sediments, and the institutional controls existing at SRS. The Environmental Protection Agency (EPA) considers units with large volumes of low-concentration wastes, as is the case with Par Pond, to be {open_quotes}special sites.{close_quotes} Accordingly, EPA guidance establishes that the range of alternatives developed focus primarily on containment options and other remedial approaches that mitigate potential risks associated with the {open_quotes}special site.{close_quotes} The remedial alternatives, according to EPA, are not to be prohibitively expensive or difficult to implement. This case study also is representative of the types of issues that will need to be addressed within the Department of Energy (DOE) complex as nuclear facilities are transitioned to inactive status and corrective/remedial actions are warranted.

  3. Identification of uPAR-positive Chemoresistant Cells in Small Cell Lung Cancer

    PubMed Central

    Gutova, Margarita; Najbauer, Joseph; Gevorgyan, Anna; Metz, Marianne Z.; Weng, Yehua; Shih, Chu-Chih; Aboody, Karen S.

    2007-01-01

    Background The urokinase plasminogen activator (uPA) and its receptor (uPAR/CD87) are major regulators of extracellular matrix degradation and are involved in cell migration and invasion under physiological and pathological conditions. The uPA/uPAR system has been of great interest in cancer research because it is involved in the development of most invasive cancer phenotypes and is a strong predictor of poor patient survival. However, little is known about the role of uPA/uPAR in small cell lung cancer (SCLC), the most aggressive type of lung cancer. We therefore determined whether uPA and uPAR are involved in generation of drug resistant SCLC cell phenotype. Methods and Findings We screened six human SCLC cell lines for surface markers for putative stem and cancer cells. We used fluorescence-activated cell sorting (FACS), fluorescence microscopy and clonogenic assays to demonstrate uPAR expression in a subpopulation of cells derived from primary and metastatic SCLC cell lines. Cytotoxic assays were used to determine the sensitivity of uPAR-positive and uPAR-negative cells to chemotherapeutic agents. The uPAR-positive cells in all SCLC lines demonstrated multi-drug resistance, high clonogenic activity and co-expression of CD44 and MDR1, putative cancer stem cell markers. Conclusions These data suggest that uPAR-positive cells may define a functionally important population of cancer cells in SCLC, which are resistant to traditional chemotherapies, and could serve as critical targets for more effective therapeutic interventions in SCLC. PMID:17327908

  4. The polarity protein Par-3 directly interacts with p75NTR to regulate myelination.

    PubMed

    Chan, Jonah R; Jolicoeur, Christine; Yamauchi, Junji; Elliott, Jimmy; Fawcett, James P; Ng, Benjamin K; Cayouette, Michel

    2006-11-01

    Cell polarity is critical in various cellular processes ranging from cell migration to asymmetric cell division and axon and dendrite specification. Similarly, myelination by Schwann cells is polarized, but the mechanisms involved remain unclear. Here, we show that the polarity protein Par-3 localizes asymmetrically in Schwann cells at the axon-glial junction and that disruption of Par-3 localization, by overexpression and knockdown, inhibits myelination. Additionally, we show that Par-3 directly associates and recruits the p75 neurotrophin receptor to the axon-glial junction, forming a complex necessary for myelination. Together, these results point to a critical role in the establishment of cell polarity for myelination. PMID:17082460

  5. Thrombin induces rapid PAR1-mediated non-classical FGF1 release

    SciTech Connect

    Duarte, Maria [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Kolev, Vihren [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Soldi, Raffaella [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Kirov, Alexander [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Graziani, Irene [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Oliveira, Silvia Marta [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Kacer, Doreen [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Friesel, Robert [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Maciag, Thomas [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States); Prudovsky, Igor [Maine Medical Center Research Institute, 81 Research Drive, Scarborough, ME 04074 (United States)]. E-mail: prudoi@mmc.org

    2006-11-24

    Thrombin induces cell proliferation and migration during vascular injury. We report that thrombin rapidly stimulated expression and release of the pro-angiogenic polypeptide fibroblast growth factor 1 (FGF1). Thrombin failed to induce FGF1 release from protease-activated receptor 1 (PAR1) null fibroblasts, indicating that this effect was dependent on PAR1. Similarly to thrombin, FGF1 expression and release were induced by TRAP, a specific oligopeptide agonist of PAR1. These results identify a novel aspect of the crosstalk between FGF and thrombin signaling pathways which both play important roles in tissue repair and angiogenesis.

  6. Arrêt cardiocirculatoire par accidents d’électrisations: intérêt du défibrillateur semi-automatique

    PubMed Central

    Siah, S.; Fouadi, F.E.; Ababou, K.; Ihrai, I.; Drissi, N.K.

    2011-01-01

    Summary Les brûlures par accidents électriques sont graves car elles peuvent entraîner le décès par arrêt cardiocirculatoire. Les arrêts cardiocirculatoires induits par le courant de basse tension sont en règle générale dûs à une fibrillation ventriculaire, plutôt de bon pronostic si la chaîne des secours est efficace. Il faut donner la priorité à la défibrillation systématique d’emblée en utilisant un défibrillateur semi-automatique. La défibrillation électrique est susceptible de procurer immédiatement une restauration de l’activité circulatoire spontanée. PMID:21991238

  7. Etude des magnétites dopéesa`la chaux: Substitution du fer par le calcium en site tétraédrique

    NASA Astrophysics Data System (ADS)

    G´rardin, R.; Bonazebi, A.; Millon, E.; Brice, J. F.; Evrard, O.; Sanchez, J. P.

    1989-01-01

    A 1200°C, par réaction entre solides, il est possible de dissoudre partiellement le ferrite monocalcique CaFe 2O 4 dans la magnétite Fe 3O 4. Le taux maximum de substitution du cation Fe 2+ par le cation Ca 2+ est de 20% atomique. Dans la solution solide de type spinelle, les ions Ca 2+ se placent presque exclusivement en site tétraédrique. Cette distribution cationique est confirmée par l'analyse des propriétés radiocristallographiques, spectrométrie Mössbauer du 57Fe et des propriétés magnétiques.

  8. Dynamic interplay of ParA with the polarity protein, Scy, coordinates the growth with chromosome segregation in Streptomyces coelicolor

    PubMed Central

    Ditkowski, Bartosz; Holmes, Neil; Rydzak, Joanna; Donczew, Magdalena; Bezulska, Martyna; Ginda, Katarzyna; K?dzierski, Pawe?; Zakrzewska-Czerwi?ska, Jolanta; Kelemen, Gabriella H.; Jakimowicz, Dagmara

    2013-01-01

    Prior to bacterial cell division, the ATP-dependent polymerization of the cytoskeletal protein, ParA, positions the newly replicated origin-proximal region of the chromosome by interacting with ParB complexes assembled on parS sites located close to the origin. During the formation of unigenomic spores from multi-genomic aerial hyphae compartments of Streptomyces coelicolor, ParA is developmentally triggered to form filaments along the hyphae; this promotes the accurate and synchronized segregation of tens of chromosomes into prespore compartments. Here, we show that in addition to being a segregation protein, ParA also interacts with the polarity protein, Scy, which is a component of the tip-organizing centre that controls tip growth. Scy recruits ParA to the hyphal tips and regulates ParA polymerization. These results are supported by the phenotype of a strain with a mutant form of ParA that uncouples ParA polymerization from Scy. We suggest that the ParA–Scy interaction coordinates the transition from hyphal elongation to sporulation. PMID:23536551

  9. Stockage de l'nergie solaire : simulation numrique du transfert d'nergie par conduction et rayonnement dans un milieu deux phases

    E-print Network

    Paris-Sud XI, Université de

    système est constitué par de la paraffine fondant vers 52 °C et soumis au rayonnement solaire par l présente les caractéristiques suivantes : - le rayonnement solaire incident est directement capté par la125 Stockage de l'énergie solaire : simulation numérique du transfert d'énergie par conduction et

  10. SUR L'INTERACTION ENTRE NEUTRONS ET PROTONS Par J. SOLOMON.

    E-print Network

    Boyer, Edmond

    par 21t; nous pren- drons pour unités de longueur et d'énergie Les mesures des grandeurs rapportées à. Nous revien- drons là-dessus un peu plus loin. En tout cas il est intéressant de remarquer que toutes

  11. Scurisation d'image par crypto-tatouage William PUECH Jos Marconi RODRIGUES

    E-print Network

    Paris-Sud XI, Université de

    .puech@lirmm.fr, jose-marconi.rodrigues@lirmm.fr Résumé Cet article présente une nouvelle méthode combinant le chif- frement d'images nécessitent le transfert de la clef secrète par un autre canal ou un autre moyen

  12. Mice lacking the thrombin receptor, PAR1, have normal skin wound healing.

    PubMed Central

    Connolly, A. J.; Suh, D. Y.; Hunt, T. K.; Coughlin, S. R.

    1997-01-01

    Thrombin's actions on platelets, macrophages, fibroblasts, and endothelial cells have prompted the hypothesis that thrombin may be important for inflammatory and fibroproliferative processes in wound healing. Protease-activated receptor 1 (PAR1) is a G-protein-coupled receptor that mediates many of the cellular activities of thrombin. To test the role of this receptor in vivo, we generated PAR1-deficient mice. Despite the observation that fibroblasts cultured from these mice lacked responsiveness to thrombin in vitro, we now report that there was no difference detected between wild-type and PAR1-deficient mice in skin wound healing assays including time to closure of open wounds, tensile strength of healed incisional wounds, wound histology, and hydroxyproline/DNA content of wound implants. We conclude that PAR1 is not necessary for normal skin wound healing in mice. Images Figure 2 PMID:9358744

  13. Substantia nigra pars reticulata neurons code initiation of a serial pattern: implications for natural action sequences

    E-print Network

    Berridge, Kent

    the elemental motor parameters in determining SNpr neuronal activation. Introduction The substantia nigra pars, Switzerland 2 Physiological Science, 4117 LSB, University of California, Los Angeles, Los Angeles, CA 90095

  14. COEFFICIENTS DE CORRECTION DES VOLUMES GAZEUX; PAR MM. N. GRHANT ET E. MER.

    E-print Network

    Paris-Sud XI, Université de

    222 COEFFICIENTS DE CORRECTION DES VOLUMES GAZEUX; PAR MM. N. GRÉHANT ET E. MER. Pour ramener à échelle graduée sur verre étant H, la température de la cuve à mer- cure ou à eau, sur laquelle on a fait

  15. DESTRUCTION PAR CHOCS LECTRONIQUES DES ATOMES MTASTABLES ET PSEUDO-MTASTABLES

    E-print Network

    Paris-Sud XI, Université de

    -Bernard Lyon I, Laboratoire de Spectroscopie et de Luminescence, 43, bd du 11-Novembre-1918, 69621 Villeurbanne destruc- tion par chocs electroniques des niveaux meta- stables ls3 et Iss, et pseudo-metastables ls4 (Fig

  16. Actes JFPC 2009 Subsumption dirige par l'analyse de conflits

    E-print Network

    Paris-Sud XI, Université de

    Actes JFPC 2009 Subsumption dirigée par l'analyse de conflits Youssef Hamadi1 Said Jabbour2 Lakhdar'Artois rue Jean Souvraz SP18 F-62307 Lens Cedex France youssefh@microsoft.com {jabbour,sais}@cril.fr Abstract

  17. Texte publi par : JEANNODA, V., RAKOTONIRINA, O., RANDRIANARIVO, H., RAKOTO, D., WRIGHT, P. & HLADIK, C.M. (2003) Le principe toxique du bambou consomm par Hapalemur aureus n'est pas neutralis par la

    E-print Network

    Boyer, Edmond

    2003-01-01

    Texte publié par : JEANNODA, V., RAKOTONIRINA, O., RANDRIANARIVO, H., RAKOTO, D., WRIGHT, P, Hanitra RANDRIANARIVO1, Danielle RAKOTO1, Patricia WRIGHT2, & Claude Marcel HLADIK3 1 Université d, Wright et al., 1987) se nourrit essentiellement des repousses d'un bambou dont la toxicité est bien

  18. UN CIRCUIT DE CONCIDENCE RAPIDE AVEC UN ANALYSEUR DE TEMPS Par ERNST RMY et KLAUS WINTER,

    E-print Network

    Boyer, Edmond

    UN CIRCUIT DE COÏNCIDENCE RAPIDE AVEC UN ANALYSEUR DE TEMPS Par ERNST R�MY et KLAUS WINTER précision de détermination des énergies de neutrons avec la méthode de temps de vol dépend essentiellement voldonnée par .E t Avec un temps de vol de 50 mys, pour des neutrons de 2 MeV sur un parcours de 1 m, et

  19. DOSAGE DU RADON DANS L'AIR. Par Mme G. DELIBRIAS,

    E-print Network

    Paris-Sud XI, Université de

    78 A. DOSAGE DU RADON DANS L'AIR. Par Mme G. DELIBRIAS, Commissariat à l'Énergie atomique. Centre d'Études Nucléaires, à Saclay. Sommaire. 2014 On décrit une méthode de dosage de traces de radon dans l'atmosphère. La. Introduction. - L'air contient normalement une certaine quantité de Radon, de l'ordre de i o-1° Curie par m3 d

  20. Model parameterization to simulate and compare the PAR absorption potential of two competing plant species

    Microsoft Academic Search

    Jörg Bendix; Brenner Silva; Kristin Roos; Dietrich Otto Göttlicher; Rütger Rollenbeck; Thomas Nauß; Erwin Beck

    2010-01-01

    Mountain pastures dominated by the pasture grass Setaria sphacelata in the Andes of southern Ecuador are heavily infested by southern bracken (Pteridium arachnoideum), a major problem for pasture management. Field observations suggest that bracken might outcompete the grass due to its competitive\\u000a strength with regard to the absorption of photosynthetically active radiation (PAR). To understand the PAR absorption potential\\u000a of

  1. UNIVERSITE D'ANGERS Annee 2006 OPTIMISATION DES PROCEDES DE MISE EN FORME PAR

    E-print Network

    Paris-Sud XI, Université de

    UNIVERSITE D'ANGERS Ann´ee 2006 N°327 OPTIMISATION DES PROCEDES DE MISE EN FORME PAR APPROCHE´ecialit´e : Sciences de l'Ing´enieur ECOLE DOCTORALE D'ANGERS Pr´esent´ee et soutenue publiquement Le 21 D´ecembre 2006 A l'Institut des Sciences et Techniques de l'Ing´enieur d'Angers Par Daniel LEPADATU Devant le jury ci

  2. SUR UN LECTROMTRE ASTATIQUE POUVANT SERVIR COMME WATTMTRE; PAR MM. R. BLONDLOT ET P. CURIE.

    E-print Network

    Boyer, Edmond

    80 SUR UN �LECTROM�TRE ASTATIQUE POUVANT SERVIR COMME WATTM�TRE; PAR MM. R. BLONDLOT ET P. CURIE peut servir par la méthode idiostatique, en unissant res- pectivement les deux paires de plateaux aux deux demi-cercles de l'aiguille ; on a alors nécessairement 3.1 11 peut servir comme wattnzèli-e. L

  3. QUANTITS D'HERBE ET DE LAIT CONSOMMES PAR DES VEAUX AU PIS

    E-print Network

    Paris-Sud XI, Université de

    QUANTITÉS D'HERBE ET DE LAIT CONSOMMÉES PAR DES VEAUX AU PIS P. LE NEINDRE* M. PETIT A. MULLER* G de lait et d'herbe consommées entre l'âge de 3 et 8 mois par 96 veaux, Novmands ou croisés Claavolais,5 mois qu'entre 5,5 et 8 mois. La consommation d'herbe augmente considérablement entre 3 et 8 mois, de 1

  4. Étude d’un système simplifié de signalisation par canal sémaphore

    Microsoft Academic Search

    Pierre Lucas

    1970-01-01

    \\u000a Sommaire  L’apparition des systèmes de commutation téléphonique modernes (électroniques ou semiélectroniques) dont la commande est assurée\\u000a par des calculateurs à programme enregistré, ouvre des possibilités nouvelles à la fonction de signalisation entre les autocommutateurs.\\u000a Dans un réseau où les calculateurs de commande vont se multiplier, il est intéressant d’envisager une signalisation par échange\\u000a direct sur des voies spécialisées appelées canaux sémaphores.

  5. SUR LA DIFFUSION DU ThC" Par Mlle C. CHAMI.

    E-print Network

    Boyer, Edmond

    SUR LA DIFFUSION DU ThC" Par Mlle C. CHAMIÉ. Sommaire. 2014 Ce travail est une étude de la diffusion du ThC" rejeté par recul a à partir du ThC et recueilli sur une plaque réceptrice non chargée. Les courbes obtenues montrent que la quantité de ThC" qui diffuse dans l'air est faible et diminue très

  6. Hypertension portale compliquée d’hémorragie par rupture de varices œsophagiennes chez la femme enceinte

    Microsoft Academic Search

    Latifa Elkihal; S. El Jastimi; R. Afifi; A. Essaïd

    2005-01-01

    \\u000a Résumé  La prise en charge thérapeutique de l’hypertension portale en cours de grossesse compliquée d’hémorragie digestive par rupture\\u000a de varices œsophagiennes fait appel actuellement aux moyens endoscopiques: la sclérose et la ligature qui sont efficaces,\\u000a tolérables et sans danger ni pour la mère ni pour la fœtus. Nous rapportons trois cas traités avec succès par sclérothérapie:\\u000a deux patientes ont été sclérosées

  7. Identification of uPAR-positive Chemoresistant Cells in Small Cell Lung Cancer

    Microsoft Academic Search

    Margarita Gutova; Joseph Najbauer; Anna Gevorgyan; Marianne Z. Metz; Yehua Weng; Chu-Chih Shih; Karen S. Aboody; Christopher Arendt

    2007-01-01

    BackgroundThe urokinase plasminogen activator (uPA) and its receptor (uPAR\\/CD87) are major regulators of extracellular matrix degradation and are involved in cell migration and invasion under physiological and pathological conditions. The uPA\\/uPAR system has been of great interest in cancer research because it is involved in the development of most invasive cancer phenotypes and is a strong predictor of poor patient

  8. Condensation d'ADN plasmidique par des diamines sur un substrat de graphite HOPG.

    E-print Network

    Paris-Sud XI, Université de

    Condensation d'ADN plasmidique par des diamines sur un substrat de graphite HOPG. Ce travail vise à améliorer notre connaissance des effets produits par les électrons de basse énergie (EBEs) sur l'ADN. Parmi]. Ce type d'étude requiert un bon étalement et un contrôle des dépôts d'ADN plasmidique sur des

  9. Influence de la pression sur l'ionisation des gaz Par E. ROTH

    E-print Network

    Paris-Sud XI, Université de

    , la lame d'aluminium et le couvercle sont serrés par des vis convenables entre des cuirs suiffés et'une batterie de petits accumulateurs, dont l'autre pôle est au sol. Le pla- teau inférieur B communique avec l quadrants est au sol. L'aiguille de l'électromètre est chargée par l'un des pôles d'une batterie d

  10. The role of the soluble urokinase plasminogen activator (suPAR) in children with pneumonia.

    PubMed

    Wrotek, A; Jackowska, T

    2015-04-01

    Although pneumonia is one of the most important health problems in children, there is still no widely accepted disease severity score, the data on the correlation between the conventional inflammatory markers or chest X-ray and the disease severity remain disputable, and thus, there is an urgent need for a new pneumonia biomarker. The soluble urokinase plasminogen activator (suPAR) is a soluble form of the urokinase plasminogen activator that plays an important role in the innate host defense in the pulmonary tissue. suPAR levels have been associated with a general activation of the immune system rather than with a particular etiological factor. suPAR has a high prognostic value in critically ill patients, especially with sepsis, but there is a growing number of studies focusing on suPAR in respiratory diseases. The aim of this review is to summarize the knowledge on the role of the suPAR/uPAR in lung pathology and its possible use in pneumonia in children. PMID:25602915

  11. Role of protease-activated receptors 2 (PAR2) in ocular infections and inflammation

    PubMed Central

    Tripathi, Trivendra; Alizadeh, Hassan

    2015-01-01

    Protease-activated receptors (PARs) belong to a unique family of G protein-coupled receptors (GPCRs) that are cleaved at an activation site within the N-terminal exodomain by a variety of proteinases, essentially of the serine (Ser) proteinase family. After cleavage, the new N-terminal sequence functions as a tethered ligand, which binds intramolecularly to activate the receptor and initiate signaling. Cell signals induced through the activation of PARs appear to play a significant role in innate and adoptive immune responses of the cornea, which is constantly exposed to proteinases under physiological or pathophysiological conditions. Activation of PARs interferes with all aspects of the corneal physiology such as barrier function, transports, innate and adoptive immune responses, and functions of corneal nerves. It is not known whether the proteinase released from the microorganism can activate PARs and triggers the inflammatory responses. The role of PAR2 expressed by the corneal epithelial cells and activation by serine protease released from microorganism is discussed here. Recent evidences suggest that activation of PAR2, by the serine proteinases, play an important role in innate and inflammatory responses of the corneal infection.

  12. Expression of guanylin in “pars tuberalis-specific cells” and gonadotrophs of rat adenohypophysis?

    PubMed Central

    D'Este, Loredana; Kulaksiz, Hasan; Rausch, Ulrich; Vaccaro, Rosa; Wenger, Tibor; Tokunaga, Yoshimitsu; Renda, Tindaro G.; Cetin, Yalcin

    2000-01-01

    The intestinal peptide guanylin regulates the electrolyte/water transport in the gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because guanylin also circulates in the blood, we investigated the rat hypothalamo-pituitary region for expression and cellular localization of this peptide. Reverse transcriptase–PCR analyses with guanylin-specific primers revealed expression of the peptide in the pars tuberalis and pars distalis of the pituitary. Western blotting analyses in hypophyseal tissue extracts identified the expected 12.5-kDa immunoreactive peptide by using two different region-specific guanylin antisera. Light and electron microscopic immunocytochemistry with the same antisera localized guanylin in “pars tuberalis-specific cells” in the juxtaneural pars tuberalis adjacent to nerve endings and blood vessels of the hypothalamo-pituitary portal system and in gonadotrophic cells within the distal pars tuberalis and ventrolateral part of the pars distalis. The presence and cell-specific localization of guanylin within the hypothalamo-hypophyseal system indicate that this peptide may be specifically involved in paracrine and endocrine regulatory mechanisms. PMID:10655496

  13. Examining relational empowerment for elementary school students in a yPAR program.

    PubMed

    Langhout, Regina Day; Collins, Charles; Ellison, Erin Rose

    2014-06-01

    This paper joins relational empowerment, youth empowerment, and Bridging Multiple Worlds frameworks to examine forms of relational empowerment for children in two intermediary institutions-school and a youth participatory action research after-school program (yPAR ASP). Participants were twelve children, most of whom were Latina/o and from im/migrant families, enrolled in a yPAR ASP for 2 years. A mixed-method approach was utilized; we analyzed children's interviews, self-defined goals, and their social networks to examine their experiences of relational empowerment. We conclude that children experienced each of the five relational empowerment factors-collaborative competence, bridging social divisions, facilitating others' empowerment, mobilizing networks, and passing on a legacy-in the yPAR ASP setting, and some factors in school. These experiences, however, were more pronounced in the yPAR ASP setting. Additionally, social network analyses revealed that a small but meaningful percentage of actors bridged worlds, especially home and family, but by year 2, also school and the yPAR ASP. Finally, most helpers for school-based goals came from school, but a sizable number came from family, friends, and home worlds, and by year 2, also came from the yPAR ASP. Implications range from theoretical to methodological development, including the use of social network analysis as a tool to descriptively examine relational power in context. PMID:24356900

  14. Heat Stress-Induced Disruption of Endothelial Barrier Function Is via PAR1 Signaling and Suppressed by Xuebijing Injection

    PubMed Central

    Xu, Qiulin; Liu, Jingxian; Wang, Zhenglian; Guo, Xiaohua; Zhou, Gengbiao; Liu, Yanan; Huang, Qiaobing; Su, Lei

    2015-01-01

    Increased vascular permeability leading to acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) is central to the pathogenesis of heatstroke. Protease-activated receptor 1 (PAR1), the receptor for thrombin, plays a key role in disruption of endothelial barrier function in response to extracellular stimuli. However, the role of PAR1 in heat stress-induced endothelial hyper-permeability is unknown. In this study, we measured PAR1 protein expression in heat-stressed human umbilical venous endothelial cells (HUVECs), investigated the influences of PAR1 on endothelial permeability, F-actin rearrangement, and moesin phosphorylation by inhibiting PAR1 with its siRNA, neutralizing antibody (anti-PAR1), specific inhibitor(RWJ56110), and Xuebijing injection (XBJ), a traditional Chinese medicine used for sepsis treatment, and evaluated the role of PAR1 in heatstroke-related ALI/ARDS in mice by suppressing PAR1 with RWJ56110, anti-PAR1and XBJ. We found that heat stress induced PAR1 protein expression 2h after heat stress in endothelial cells, caused the release of endothelial matrix metalloprotease 1, an activator of PAR1, after 60 or 120 min of heat stimulation, as well as promoted endothelial hyper-permeability and F-actin rearrangement, which were inhibited by suppressing PAR1 with RWJ56110, anti-PAR1 and siRNA. PAR1 mediated moesin phosphorylation, which caused F-actin rearrangement and disruption of endothelial barrier function. To corroborate findings from in vitro experiments, we found that RWJ56110 and the anti-PAR1 significantly decreased lung edema, pulmonary microvascular permeability, protein exudation, and leukocytes infiltrations in heatstroke mice. Additionally, XBJ was found to suppress PAR1-moesin signal pathway and confer protective effects on maintaining endothelial barrier function both in vitro and in vivo heat-stressed model, similar to those observed above with the inhibition of PAR1. These results suggest that PAR1 is a potential therapeutic target in heatstroke. PMID:25693178

  15. Par-4, a Gene Required for Cytoplasmic Localization and Determination of Specific Cell Types in Caenorhabditis Elegans Embryogenesis

    PubMed Central

    Morton, D. G.; Roos, J. M.; Kemphues, K. J.

    1992-01-01

    Specification of some cell fates in the early Caenorhabditis elegans embryo is mediated by cytoplasmic localization under control of the maternal genome. Using nine newly isolated mutations, and two existing mutations, we have analyzed the role of the maternally expressed gene par-4 in cytoplasmic localization. We recovered seven new par-4 alleles in screens for maternal effect lethal mutations that result in failure to differentiate intestinal cells. Two additional par-4 mutations were identified in noncomplementation screens using strains with a high frequency of transposon mobility. All 11 mutations cause defects early in development of embryos produced by homozygous mutant mothers. Analysis with a deficiency in the region indicates that it33 is a strong loss-of-function mutation. par-4(it33) terminal stage embryos contain many cells, but show no morphogenesis, and are lacking intestinal cells. Temperature shifts with the it57ts allele suggest that the critical period for both intestinal differentiation and embryo viability begins during oogenesis, about 1.5 hr before fertilization, and ends before the four-cell stage. We propose that the primary function of the par-4 gene is to act as part of a maternally encoded system for cytoplasmic localization in the first cell cycle, with par-4 playing a particularly important role in the determination of intestine. Analysis of a par-4;par-2 double mutant suggests that par-4 and par-2 gene products interact in this system. PMID:1582558

  16. A Novel Function for the PAR Complex in Subcellular Morphogenesis of Tracheal Terminal Cells in Drosophila melanogaster

    PubMed Central

    Jones, Tiffani A.; Metzstein, Mark M.

    2011-01-01

    The processes that generate cellular morphology are not well understood. To investigate this problem, we use Drosophila melanogaster tracheal terminal cells, which undergo two distinct morphogenetic processes: subcellular branching morphogenesis and subcellular apical lumen formation. Here we show these processes are regulated by components of the PAR-polarity complex. This complex, composed of the proteins Par-6, Bazooka (Par-3), aPKC, and Cdc42, is best known for roles in asymmetric cell division and apical/basal polarity. We find Par-6, Bazooka, and aPKC, as well as known interactions between them, are required for subcellular branch initiation, but not for branch outgrowth. By analysis of single and double mutants, and isolation of two novel alleles of Par-6, one of which specifically truncates the Par-6 PDZ domain, we conclude that dynamic interactions between apical PAR-complex members control the branching pattern of terminal cells. These data suggest that canonical apical PAR-complex activity is required for subcellular branching morphogenesis. In addition, we find the PAR proteins are downstream of the FGF pathway that controls terminal cell branching. In contrast, we find that while Par-6 and aPKC are both required for subcellular lumen formation, neither Bazooka nor a direct interaction between Par-6 and aPKC is needed for this process. Thus a novel, noncanonical role for the polarity proteins Par-6 and aPKC is used in formation of this subcellular apical compartment. Our results demonstrate that proteins from the PAR complex can be deployed independently within a single cell to control two different morphogenetic processes. PMID:21750259

  17. B. GILL et F.-B.-I. PIDDUCK. -Ionisation par choc clans l'hlium. -La thorie de l'ionisation par choc de Townsend a t confirme

    E-print Network

    Boyer, Edmond

    'ionisation par choc de Townsend a été confirmée expérimentalement pour un grand nombre de gaz, en particulier lGNERON. ANNALEN DER PHYSIK ; T. XXXVII, n° 5, et t. XXXVIII, n°s 6 et 7 ; 1912. W. von IGNATOWSKY. 2013 Sur la réflexion totale, t. P. 90i-9IOs W. von IGNATOBVSKY et E. OETTIKGER. - Recherches expérimentales sur la

  18. 25-Gauge pars plana vitrectomy for ciliary block (malignant) glaucoma.

    PubMed

    Meng, Li; Wei, Wenbin; Li, Yanlong; Hui, Xiaoying; Han, Xiaodong; Shi, Xiaobo

    2015-08-01

    To evaluate the efficacy of 25-ga pars plana vitrectomy (PPV) and 25-ga PPV combined with phacoemulsification surgery for managing ciliary block (malignant) glaucoma. The medical records of 11 eyes, in nine consecutive patients with malignant glaucoma were retrospectively reviewed to determine the efficacy of 25-ga PPV for alleviating this disease. All phakic eyes underwent phacoemulsification surgery during vitrectomy. Control of intraocular pressure (IOP), pre- and postoperative best-corrected visual acuity (BCVA), and development of intra- and postoperative complications were evaluated during follow-up. Malignant glaucoma was promptly resolved, and recurrence was not observed during a mean follow-up period of 12.7 ± 3.2 (range 6-18) months. The mean IOP decreased from 35.9 ± 2.9 mmHg, preoperatively, to 16.0 ± 1.4 mmHg, measured at last visit (P = 0.013). Although IOP control was achieved in all eyes after surgery, two eyes required long-term topical antiglaucoma medication. The mean logarithm of the minimum angle of resolution BCVA improved from +0.69 ± 0.11, preoperatively, to +0.29 ± 0.07, postoperatively (P = 0.027). No severe intraoperative complications were observed. Postoperative complications mostly occurred during the first week after surgery, including transient corneal edema and Descemet's membrane folds in three eyes, fibrotic exudation in 2, local iris posterior synechia in 1, and transient hypotony in 1, which were resolved spontaneously or with medication. The 25-ga PPV procedure seems to be safe and effective for treating malignant glaucoma. Combined vitrectomy with phacoemulsification surgery and a staged surgical approach may improve the success rate. PMID:25062710

  19. Douleurs induites par les soins: la réalité au Centre Hospitalier Universitaire de Befelatanana Antananarivo, Madagascar

    PubMed Central

    Mahavivola, Ernestho-Ghoud Indretsy; Olivah, Razanaparany Miarisoa Mireille; Mihary, Dodo; Hendriniaina, Rakotoharivelo; Lalao, Randriamboavonjy Rado; Henintsoa, Rakotonirainy Oliva; Fahafahantsoa, Rapelanoro Rabenja

    2014-01-01

    La douleur induite par les soins correspond à la douleur survenant lors des actes à visé diagnostique et/ou thérapeutique. A notre connaissance, nous n'avons pas encore des données disponibles pour les douleurs induites par les soins à l'Hôpital de Befelatanana. Nos objectifs étaient de décrire le profil épidémiologique de la douleur induite par les soins, d'identifier les principaux facteurs influençant sur l'intensité de la douleur et leurs retentissements chez les patients. Il s'agissait d'une étude rétrospective, transversale type un jour donné menée dans les douze services de Médecines au Centre Hospitalier Universitaire de Befelatanana en Novembre 2013. Cent deux patients ont été retenus dans l’étude et trois cent vingt trois actes douloureux étaient enregistrés. La fréquence de la douleur induite par les soins était de 69,86%. Le genre féminin prédominait dans 52% des cas (n = 53) avec un sex-ratio à 0,92. L’âge moyen était de 46 ans. Les ponctions vasculaires étaient l'acte prédominant dans 49,54% (n = 109) des cas. Les infirmiers réalisaient les soins dans 47,05% (n = 48) des cas. L'information verbale était la mesure préventive utilisée dans 57,84% des cas (n = 59). Le transport par marche à pied et au dos représentait 16,67% des cas (n = 17). Les patients naïfs des gestes étaient plus anxieux. Ces patients gardaient de mauvais souvenir dans 64,71% des cas (n = 66). La fréquence de douleur induite par les soins était trop élevée. Un effort important est nécessaire pour réduire la douleur induite par les soins PMID:25932071

  20. Proteolytic activation of the protease-activated receptor (PAR)-2 by the glycosylphosphatidylinositol-anchored serine protease testisin.

    PubMed

    Driesbaugh, Kathryn H; Buzza, Marguerite S; Martin, Erik W; Conway, Gregory D; Kao, Joseph P Y; Antalis, Toni M

    2015-02-01

    Protease-activated receptors (PARs) are a family of seven-transmembrane, G-protein-coupled receptors that are activated by multiple serine proteases through specific N-terminal proteolytic cleavage and the unmasking of a tethered ligand. The majority of PAR-activating proteases described to date are soluble proteases that are active during injury, coagulation, and inflammation. Less investigation, however, has focused on the potential for membrane-anchored serine proteases to regulate PAR activation. Testisin is a unique trypsin-like serine protease that is tethered to the extracellular membrane of cells through a glycophosphatidylinositol (GPI) anchor. Here, we show that the N-terminal domain of PAR-2 is a substrate for testisin and that proteolytic cleavage of PAR-2 by recombinant testisin activates downstream signaling pathways, including intracellular Ca(2+) mobilization and ERK1/2 phosphorylation. When testisin and PAR-2 are co-expressed in HeLa cells, GPI-anchored testisin specifically releases the PAR-2 tethered ligand. Conversely, knockdown of endogenous testisin in NCI/ADR-Res ovarian tumor cells reduces PAR-2 N-terminal proteolytic cleavage. The cleavage of PAR-2 by testisin induces activation of the intracellular serum-response element and NF?B signaling pathways and the induction of IL-8 and IL-6 cytokine gene expression. Furthermore, the activation of PAR-2 by testisin results in the loss and internalization of PAR-2 from the cell surface. This study reveals a new biological substrate for testisin and is the first demonstration of the activation of a PAR by a serine protease GPI-linked to the cell surface. PMID:25519908

  1. PaRS: Parallel and Near-Optimal Grid-Based Cell Sizing for Library-Based Design

    Microsoft Academic Search

    Tai-Hsuan Wu; Azadeh Davoodi

    2009-01-01

    We propose PaRS, a parallel and randomized al- gorithm and tool to solve the discrete gate sizing (cell sizing) problem on a grid. PaRS is formulated based on an optimization framework known as Nested Partitions which we adopt for the first time in the CAD area. PaRS uses parallelism from a novel perspective to better identify the optimization direction. It

  2. Streptococcal SpeB Cleaved PAR-1 Suppresses ERK Phosphorylation and Blunts Thrombin-Induced Platelet Aggregation

    PubMed Central

    Ender, Miriam; Andreoni, Federica; Zinkernagel, Annelies Sophie; Schuepbach, Reto Andreas

    2013-01-01

    Background The family of 4 related protease-activated receptors (PAR-1, 2, 3 & 4) expressed by mammalian cells allow to sense for and react to extracellular proteolytic activity. Since major human bacterial pathogens secret a wide array of protease(-s) we investigated whether they interfere with human PAR function. Methodology/Principal Findings Supernatants from cultures of major human bacterial pathogens were assayed for the presence of protease(-s) capable to cleave overexpressed human PAR-1, 2, 3 and 4 reporter constructs. Group A streptococcus (GAS) was found to secret a PAR-1-cleaving protease. Experiments involving genetical and pharmacological gain and loss of function identified streptococcal pyrogenic exotoxin B SpeB as the protease responsible. On the host’s side analysis of overexpressed PAR-1 carrying alanine substitutions and deletions showed the amino acid residue leucine44 on PAR-1’s extracellular N-terminus to be the only cleavage site. Complementary studies on endogenously expressed PAR-1 using PAR-1 blocking antibodies further supported our conclusion. Through PAR-1 cleavage SpeB efficiently blunted thrombin-induced induction of the ERK-pathway in endothelial cells and prevented platelets aggregation in response to thrombin. Conclusions/Significance Our results identify a novel function of the streptococcal virulence factor SpeB. By cleaving human PAR-1 at the N-terminal amino acid residue leucine44 SpeB rendered endothelial cells unresponsive to thrombin and prevented human platelets from thrombin-induced aggregation. These results suggest that by blunting PAR-1 signaling, SpeB modulates various innate host responses directed against invasive GAS potentially helping the invasive bacteria to escape. This may allow to tailor additional treatments in the future since upon invasion of the blood stream endothelial cells as well as platelets and mononuclear cells respond to PAR-1 agonists aiming to prevent further bacterial dissemination. PMID:24278414

  3. Architecture and assembly of a divergent member of the ParM family of bacterial actin like proteins

    E-print Network

    Mullins, Dyche

    proteins Christopher R. Rivera, 1 Justin M. Kollman, 2,3 Jessica K. Polka, 1 David A. Agard, 2,3 and R in a divergent member of the ParM family from plasmid pB171. Previous work demonstrated that R1 ParM assembles only 41% identical, R1 and pB171 ParMs polymerize into nearly identical filaments with similar assembly

  4. Loss of Par-1a/MARK3/C-TAK1 Kinase Leads to Reduced Adiposity, Resistance to Hepatic Steatosis, and Defective Gluconeogenesis ?

    PubMed Central

    Lennerz, Jochen K.; Hurov, Jonathan B.; White, Lynn S.; Lewandowski, Katherine T.; Prior, Julie L.; Planer, G. James; Gereau, Robert W.; Piwnica-Worms, David; Schmidt, Robert E.; Piwnica-Worms, Helen

    2010-01-01

    Par-1 is an evolutionarily conserved protein kinase required for polarity in worms, flies, frogs, and mammals. The mammalian Par-1 family consists of four members. Knockout studies of mice implicate Par-1b/MARK2/EMK in regulating fertility, immune homeostasis, learning, and memory as well as adiposity, insulin hypersensitivity, and glucose metabolism. Here, we report phenotypes of mice null for a second family member (Par-1a/MARK3/C-TAK1) that exhibit increased energy expenditure, reduced adiposity with unaltered glucose handling, and normal insulin sensitivity. Knockout mice were protected against high-fat diet-induced obesity and displayed attenuated weight gain, complete resistance to hepatic steatosis, and improved glucose handling with decreased insulin secretion. Overnight starvation led to complete hepatic glycogen depletion, associated hypoketotic hypoglycemia, increased hepatocellular autophagy, and increased glycogen synthase levels in Par-1a?/? but not in control or Par-1b?/? mice. The intercrossing of Par-1a?/? with Par-1b?/? mice revealed that at least one of the four alleles is necessary for embryonic survival. The severity of phenotypes followed a rank order, whereby the loss of one Par-1b allele in Par-1a?/? mice conveyed milder phenotypes than the loss of one Par-1a allele in Par-1b?/? mice. Thus, although Par-1a and Par-1b can compensate for one another during embryogenesis, their individual disruption gives rise to distinct metabolic phenotypes in adult mice. PMID:20733003

  5. PAR3 and aPKC regulate Golgi organization through CLASP2 phosphorylation to generate cell polarity

    PubMed Central

    Matsui, Toshinori; Watanabe, Takashi; Matsuzawa, Kenji; Kakeno, Mai; Okumura, Nobumasa; Sugiyama, Ikuko; Itoh, Norimichi; Kaibuchi, Kozo

    2015-01-01

    The organization of the Golgi apparatus is essential for cell polarization and its maintenance. The polarity regulator PAR complex (PAR3, PAR6, and aPKC) plays critical roles in several processes of cell polarization. However, how the PAR complex participates in regulating the organization of the Golgi remains largely unknown. Here we demonstrate the functional cross-talk of the PAR complex with CLASP2, which is a microtubule plus-end–tracking protein and is involved in organizing the Golgi ribbon. CLASP2 directly interacted with PAR3 and was phosphorylated by aPKC. In epithelial cells, knockdown of either PAR3 or aPKC induced the aberrant accumulation of CLASP2 at the trans-Golgi network (TGN) concomitantly with disruption of the Golgi ribbon organization. The expression of a CLASP2 mutant that inhibited the PAR3-CLASP2 interaction disrupted the organization of the Golgi ribbon. CLASP2 is known to localize to the TGN through its interaction with the TGN protein GCC185. This interaction was inhibited by the aPKC-mediated phosphorylation of CLASP2. Furthermore, the nonphosphorylatable mutant enhanced the colocalization of CLASP2 with GCC185, thereby perturbing the Golgi organization. On the basis of these observations, we propose that PAR3 and aPKC control the organization of the Golgi through CLASP2 phosphorylation. PMID:25518939

  6. Endothelial progenitor cell-dependent angiogenesis requires localization of the full-length form of uPAR in caveolae.

    PubMed

    Margheri, Francesca; Chillà, Anastasia; Laurenzana, Anna; Serratì, Simona; Mazzanti, Benedetta; Saccardi, Riccardo; Santosuosso, Michela; Danza, Giovanna; Sturli, Niccolò; Rosati, Fabiana; Magnelli, Lucia; Papucci, Laura; Calorini, Lido; Bianchini, Francesca; Del Rosso, Mario; Fibbi, Gabriella

    2011-09-29

    Endothelial urokinase-type plasminogen activator receptor (uPAR) is thought to provide a regulatory mechanism in angiogenesis. Here we studied the proangiogenic role of uPAR in endothelial colony-forming cells (ECFCs), a cell population identified in human umbilical blood that embodies all of the properties of an endothelial progenitor cell matched with a high proliferative rate. By using caveolae-disrupting agents and by caveolin-1 silencing, we have shown that the angiogenic properties of ECFCs depend on caveolae integrity and on the presence of full-length uPAR in such specialized membrane invaginations. Inhibition of uPAR expression by antisense oligonucleotides promoted caveolae disruption, suggesting that uPAR is an inducer of caveolae organization. Vascular endothelial growth factor (VEGF) promoted accumulation of uPAR in ECFC caveolae in its undegraded form. We also demonstrated that VEGF-dependent ERK phosphorylation required integrity of caveolae as well as caveolar uPAR expression. VEGF activity depends on inhibition of ECFC MMP12 production, which results in impairment of MMP12-dependent uPAR truncation. Further, MMP12 overexpression in ECFC inhibited vascularization in vitro and in vivo. Our data suggest that intratumor homing of ECFCs suitably engineered to overexpress MMP12 could have the chance to control uPAR-dependent activities required for tumor angiogenesis and malignant cells spreading. PMID:21803847

  7. Nanomechanical recognition of prognostic biomarker suPAR with DVD-ROM optical technology

    NASA Astrophysics Data System (ADS)

    Bache, Michael; Bosco, Filippo G.; Brøgger, Anna L.; Frøhling, Kasper B.; Sonne Alstrøm, Tommy; Hwu, En-Te; Chen, Ching-Hsiu; Eugen-Olsen, Jesper; Hwang, Ing-Shouh; Boisen, Anja

    2013-11-01

    In this work the use of a high-throughput nanomechanical detection system based on a DVD-ROM optical drive and cantilever sensors is presented for the detection of urokinase plasminogen activator receptor inflammatory biomarker (uPAR). Several large scale studies have linked elevated levels of soluble uPAR (suPAR) to infectious diseases, such as HIV, and certain types of cancer. Using hundreds of cantilevers and a DVD-based platform, cantilever deflection response from antibody-antigen recognition is investigated as a function of suPAR concentration. The goal is to provide a cheap and portable detection platform which can carry valuable prognostic information. In order to optimize the cantilever response the antibody immobilization and unspecific binding are initially characterized using quartz crystal microbalance technology. Also, the choice of antibody is explored in order to generate the largest surface stress on the cantilevers, thus increasing the signal. Using optimized experimental conditions the lowest detectable suPAR concentration is currently around 5 nM. The results reveal promising research strategies for the implementation of specific biochemical assays in a portable and high-throughput microsensor-based detection platform.

  8. Rassf5 and Ndr kinases regulate neuronal polarity through Par3 phosphorylation in a novel pathway

    PubMed Central

    Yang, Rui; Kong, Eryan; Jin, Jing; Hergovich, Alexander; Püschel, Andreas W.

    2014-01-01

    ABSTRACT The morphology and polarized growth of cells depend on pathways that control the asymmetric distribution of regulatory factors. The evolutionarily conserved Ndr kinases play important roles in cell polarity and morphogenesis in yeast and invertebrates but it is unclear whether they perform a similar function in mammalian cells. Here, we analyze the function of mammalian Ndr1 and Ndr2 (also known as STK38 or STK38L, respectively) in the establishment of polarity in neurons. We show that they act downstream of the tumor suppressor Rassf5 and upstream of the polarity protein Par3 (also known as PARD3). Rassf5 and Ndr1 or Ndr2 are required during the polarization of hippocampal neurons to prevent the formation of supernumerary axons. Mechanistically, the Ndr kinases act by phosphorylating Par3 at Ser383 to inhibit its interaction with dynein, thereby polarizing the distribution of Par3 and reinforcing axon specification. Our results identify a novel Rassf5–Ndr–Par3 signaling cascade that regulates the transport of Par3 during the establishment of neuronal polarity. Their role in neuronal polarity suggests that Ndr kinases perform a conserved function as regulators of cell polarity. PMID:24928906

  9. Platelet Matrix Metalloprotease-1 Mediates Thrombogenesis By Activating PAR1 at a Cryptic Ligand Site

    PubMed Central

    Trivedi, Vishal; Boire, Adrienne; Tchernychev, Boris; Kaneider, Nicole C.; Leger, Andrew J.; O’Callaghan, Katie; Covic, Lidija; Kuliopulos, Athan

    2009-01-01

    Matrix metalloproteases (MMPs) play many important roles in normal and pathological remodeling processes including atherothrombotic disease, inflammation, angiogenesis and cancer. Traditionally, MMPs have been viewed as matrix-degrading enzymes, but recent studies have shown that they possess direct signaling capabilities. Platelets harbor several MMPs that modulate hemostatic function and platelet survival, however their mode of action remains unknown. We demonstrated that platelet MMP-1 activates protease-activated receptor-1 (PAR1) on the surface of platelets. Exposure of platelets to fibrillar collagen converts the surface-bound proMMP-1 zymogen to active MMP-1 which promotes aggregation through PAR1. Unexpectedly, we found that MMP-1 cleaved PAR1 at a novel site which strongly activated Rho-GTP pathways, cell shape change and motility, and MAPK signaling. Blockade of MMP1-PAR1 greatly curtailed thrombogenesis under arterial flow conditions and inhibited thrombosis in animals. These studies provide a link between matrix-dependent activation of metalloproteases and platelet-G protein signaling and identify MMP1-PAR1 as a new target for the prevention of arterial thrombosis. PMID:19379698

  10. Lipid nanoparticles as delivery vehicles for the Parietaria judaica major allergen Par j 2

    PubMed Central

    Bondì, Maria Luisa; Montana, Giovanna; Craparo, Emanuela Fabiola; Di Gesù, Roberto; Giammona, Gaetano; Bonura, Angela; Colombo, Paolo

    2011-01-01

    Parietaria pollen is one of the major causes of allergic reaction in southern Europe, affecting about 30% of all allergic patients in this area. Specific immunotherapy is the only treatment able to modify the natural outcome of the disease by restoring a normal immunity against allergens. The preparation of allergen-solid lipid nanoparticles as delivery vehicles for therapeutic proteins, P. judaica major allergen Par j 2, was investigated. The Par j 2 allergen was expressed in a large amount in Escherichia coli and purified to homogeneity. Its immunological properties were studied by western blotting and enzyme-linked immunosorbent assay inhibition. Solid lipid nanoparticles were obtained by water-in-oil-in-water multiple emulsion method and characterized in terms of mean size and surface charge. These systems (approximately 250 nm diameter and negative surface charge) incorporated recombinant Par j 2 with 40% or greater efficiency. Moreover, the endotoxin level and anaphylactic activity of the empty solid lipid nanoparticles and recombinant Par j 2-loaded solid lipid nanoparticles were evaluated by looking at the overexpression of CD203c marker on human basophils. These results demonstrate that recombinant Par j 2-nanoparticles could be proposed as safe compositions for the development of new therapeutic dosage forms to cure allergic reactions. PMID:22162654

  11. "It was like reading a detective novel": Using PAR to work together for culture change.

    PubMed

    Fortune, Darla; McKeown, Janet; Dupuis, Sherry; de Witt, Lorna

    2015-08-01

    Participatory action research (PAR), with its focus on engagement and collaboration, is uniquely suited to enhancing culture change initiatives in dementia care. Yet, there is limited literature of its application to culture change approaches in care settings, and even less in dementia specific care contexts. To address these gaps in the literature, the purpose of this paper is to examine the complexities of a PAR project aimed at changing the culture of dementia care in two diverse dementia care settings, including a long term care (LTC) and community care setting. Drawing from data gathered throughout the PAR process, we unpack the challenges experienced by participants working together to guide culture change within their respective care settings. These challenges include: overextending selves through culture change participation; fluctuating group membership; feeling uncertainty, confusion and apprehension about the process; frustratingly slow process; and seeking diverse group representation in decision making. We also highlight the potential for appreciative inquiry (AI) to be integrated with PAR to guide a process whereby participants involved in culture change initiatives can develop strategies to mitigate challenges they experience. We view the challenges and strategies shared here as being constructive to would-be culture change agents and hope this paper will move others to consider the use of PAR when engaging in culture change initiatives. PMID:26162724

  12. Targeting of peptide conjugated magnetic nanoparticles to urokinase plasminogen activator receptor (uPAR) expressing cells.

    PubMed

    Hansen, Line; Larsen, Esben Kjær Unmack; Nielsen, Erik Holm; Iversen, Frank; Liu, Zhuo; Thomsen, Karen; Pedersen, Michael; Skrydstrup, Troels; Nielsen, Niels Chr; Ploug, Michael; Kjems, Jørgen

    2013-09-01

    Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are currently being used as a magnetic resonance imaging (MRI) contrast agent in vivo, mainly by their passive accumulation in tissues of interest. However, a higher specificity can ideally be achieved when the nanoparticles are targeted towards cell specific receptors and this may also facilitate specific drug delivery by an enhanced target-mediated endocytosis. We report efficient peptide-mediated targeting of magnetic nanoparticles to cells expressing the urokinase plasminogen activator receptor (uPAR), a surface biomarker for poor patient prognosis shared by several cancers including breast, colorectal, and gastric cancers. Conjugation of a uPAR specific targeting peptide onto polyethylene glycol (PEG) coated USPIO nanoparticles by click chemistry resulted in a five times higher uptake in vitro in a uPAR positive cell line compared to nanoparticles carrying a non-binding control peptide. In accordance with specific receptor-mediated recognition, a low uptake was observed in the presence of an excess of ATF, a natural ligand for uPAR. The uPAR specific magnetic nanoparticles can potentially provide a useful supplement for tumor patient management when combined with MRI and drug delivery. PMID:23835641

  13. Participatory Action Research (PAR) in Middle School: Opportunities, Constraints, and Key Processes

    PubMed Central

    Ritterman, Miranda L.; Wanis, Maggie G.

    2010-01-01

    Late childhood and early adolescence represent a critical transition in the developmental and academic trajectory of youth, a time in which there is an upsurge in academic disengagement and psychopathology. PAR projects that can promote youth’s sense of meaningful engagement in school and a sense of efficacy and mattering can be particularly powerful given the challenges of this developmental stage. In the present study, we draw on data from our own collaborative implementation of PAR projects in secondary schools to consider two central questions: (1) How do features of middle school settings and the developmental characteristics of the youth promote or inhibit the processes, outcomes, and sustainability of the PAR endeavor? and (2) How can the broad principles and concepts of PAR be effectively translated into specific intervention activities in schools, both within and outside of the classroom? In particular, we discuss a participatory research project conducted with 6th and 7th graders at an urban middle school as a means of highlighting the opportunities, constraints, and lessons learned in our efforts to contribute to the high-quality implementation and evaluation of PAR in diverse urban public schools. PMID:20676754

  14. Molecular Dynamics Simulations and Functional Characterization of the Interactions of the PAR2 Ectodomain with Factor VIIa

    PubMed Central

    Zhang, Qing; Petersen, Helle H.; Ostergaard, Henrik; Ruf, Wolfram; Olson, Arthur J.

    2010-01-01

    Signaling of the tissue factor-FVIIa complex regulates angiogenesis, tumor growth and inflammation. TF-FVIIa triggers cell signaling events by cleavage of protease activated receptor (PAR2) at the Arg36-Ser37 scissile bond. The recognition of PAR2 by the FVIIa protease domain is poorly understood. We perform molecular modeling and dynamics simulations to derive the PAR2-FVIIa interactions. Docking of the PAR2 Arg36-Ser37 scissile bond to the S1 site and subsequent molecular dynamics leads to interactions of the PAR2 ectodomain with P and P’ sites of the FVIIa catalytic cleft as well as to electrostatic interactions between a stably folded region of PAR2 and a cluster of basic residues remote from the catalytic cleft of FVIIa. To address the functional significance of this interaction for PAR2 cleavage, we employed two antibodies with epitopes previously mapped to this cluster of basic residues. Although these antibodies do not block the catalytic cleft, both antibodies completely abrogated PAR2 activation by TF-FVIIa. Our simulations indicate a conformation of the PAR2 ectodomain that limits the cleavage site to no more than 33 A from its membrane proximal residue. Since the active site of FVIIa in the TF-FVIIa complex is ~ 75A above the membrane, cleavage of the folded conformation of PAR2 would require tilting of the TF-FVIIa complex toward the membrane, indicating that additional cellular factors may be required to properly align the scissile bond of PAR2 with TF-FVIIa. PMID:19536895

  15. The anticonvulsant gabapentin decreases firing rates of substantia nigra pars reticulata neurons.

    PubMed

    Bloms-Funke, P; Löscher, W

    1996-12-01

    Gabapentin is a novel anti-epileptic drug which enhances GABA (gamma-aminobutyric acid) turnover in certain brain regions, including substantia nigra. However, the functional consequences of GABA turnover increases in response to gabapentin and their potential involvement in the anticonvulsant action of this drug are not known. In the present study, we examined the effects of gabapentin on the extracellular, single unit activity of nondopaminergic (presumably GABAergic) neurons of the substantia nigra pars reticulata in rats. During the recordings, the animals were infused with the narcotic opioid analgesic fentanyl, associated with a skeletal muscle relaxant and artificial ventilation. The spontaneous firing of substantia nigra pars reticulata neurons was determined up to about 2 h after i.v. or i.p. administration of gabapentin at doses of 15-30 mg/kg. After both routes of administration, gabapentin markedly reduced neuronal firing when administered at a dose of 20-30 mg/kg, while 15 mg/kg were ineffective in this regard. The suppressive effect of gabapentin was rapid in onset (2 min after i.v. and about 20 min after i.p. injection), reached peak values of about 70% below predrug baseline after about 45-60 min, and remained at this level for at least 2 h. Vehicle administration had no effect on substantia nigra pars reticulata neurons. The ability of gabapentin to alter substantia nigra pars reticulata firing does correlate with its known ability to increase nigral GABA turnover. Since a substantial body of evidence suggests that the substantia nigra pars reticulata is a critical site at which decrease of neuronal firing by potentiation of GABAergic influences results in protection against various seizure types, the suppressive effect of gabapentin on substantia nigra pars reticulata activity may contribute to the anticonvulsant action of this drug. PMID:8982688

  16. PAR6B is required for tight junction formation and activated PKC? localization in breast cancer.

    PubMed

    Cunliffe, Heather E; Jiang, Yuan; Fornace, Kimberly M; Yang, Fan; Meltzer, Paul S

    2012-01-01

    Dysregulation of mechanisms that govern the control of epithelial cell polarity, morphology and plasticity are emerging as key processes in tumor progression. In this study we report amplification and overexpression of PAR6B, an essential component in epithelial cell tight junction (TJ) formation and maintenance of apico-basal polarity, in breast cancer cell lines. Analysis of chromosome 20q13.13 in 11 breast cancer cell lines by fluorescence in situ hybridization (FISH) identified a novel small amplicon centered at PARD6B in 5 cell lines, with copy number ranging from 7 to 27. The presence of the PARD6B amplicon correlated with PARD6B transcript and PAR6B protein abundance. Expression of related isoforms PARD6A and PARD6G were detectable at significantly lower levels. PARD6B overexpression correlated with TJ network formation in cultured cell monolayers. SiRNA-mediated inhibition of PAR6B in MCF7 resulted in loss of TJ assembly and membrane localization of atypical PKC? (aPKC), but did not affect adherens junction formation. SiRNA-mediated inhibition of CDC42 in MCF7 also resulted in loss of TJ networks, confirming the requirement of a complete PAR6-aPKC-CDC42-PAR3 complex to activate and stabilize TJs. Immunohistochemical analysis of PAR6B expression on breast tumor microarrays indicated exquisite epithelial cell-specificity. Few quantitative differences in staining were observed between normal epithelium and adjacent tumor margins. However staining appeared reduced and cytoplasmic in more poorly differentiated tumors. We propose that quantitative imbalances in the components of pathways governing normal epithelial cell polarity arising from gain or loss of function may radically alter epithelial cell architecture and contribute to tumor progression. PMID:22957302

  17. Factor Xa stimulates fibroblast procollagen production, proliferation, and calcium signaling via PAR{sub 1} activation

    SciTech Connect

    Blanc-Brude, Olivier P. [Royal Free and University College London Medical School, Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, 5 University Street, London WC1E 6JJ (United Kingdom)]. E-mail: olivier.blanc-brude@larib.inserm.fr; Archer, Fabienne [University College London Department of Physiology, Bolsover Laboratory, Gower Street, London WC1E 6BT (United Kingdom); Leoni, Patricia [Royal Free and University College London Medical School, Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, 5 University Street, London WC1E 6JJ (United Kingdom); Derian, Claudia [Johnson and Johnson Pharmaceutical Research and Development, LLC, Welsch and McKean roads, Spring House, PA 19477-0776 (United States); Bolsover, Steven [University College London Department of Physiology, Bolsover Laboratory, Gower Street, London WC1E 6BT (United Kingdom); Laurent, Geoffrey J. [Royal Free and University College London Medical School, Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, 5 University Street, London WC1E 6JJ (United Kingdom); Chambers, Rachel C. [Royal Free and University College London Medical School, Centre for Cardiopulmonary Biochemistry and Respiratory Medicine, 5 University Street, London WC1E 6JJ (United Kingdom)

    2005-03-10

    Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR{sub 1}). During hemostasis, the coagulation cascade proteinase factor X is converted into factor Xa. We have previously shown that factor Xa upregulates fibroblast proliferation via production of autocrine PDGF. In this study, we further examined the effects of factor Xa on fibroblast function and aimed to identify its signaling receptor. We showed that factor Xa stimulates procollagen promoter activity and protein production by human and mouse fibroblasts. This effect was independent of PDGF and thrombin production, but dependent on factor Xa proteolytic activity. We also showed that PAR{sub 1}-deficient mouse fibroblasts did not upregulate procollagen production, mobilize cytosolic calcium, or proliferate in response to factor Xa. Desensitization techniques and PAR{sub 1}-specific agonists and inhibitors were used to demonstrate that PAR{sub 1} mediates factor Xa signaling in human fibroblasts. This is the first report that factor Xa stimulates extracellular matrix production. In contrast with endothelial cells and vascular smooth muscle cells, fibroblasts appear to be the only cell type in which the effects of factor Xa are mediated mainly via PAR{sub 1} and not PAR{sub 2}. These findings are critical for our understanding of tissue repair and fibrotic mechanisms, and for the design of novel approaches to inhibit the profibrotic effects of the coagulation cascade without compromising blood hemostasis.

  18. Prdtermination d'une prfrence alimentaire par stimulation olfactive post-partum. Premiers rsultats chez la ratte, par Michle CORMARECHE-LEYDIER, Arlette DUVEAU,Michle CORMARECHE-LEYDIER Arlette DUVEAU

    E-print Network

    Paris-Sud XI, Université de

    Prédétermination d'une préférence alimentaire par stimulation olfactive post-partum. Premiers-Bernard/yon,...... ,1 - ...... _ ... ! '-1 - _ F-69622 Villeurbanne Cedex, France. L'effet de stimulations olfactives long du nycthémère, créant une ambiance olfactive qui se déplace avec l'animal et qu'il retrouve, par

  19. Le taux protique avait peu d'effet sur le poids corporel et la ponte par poule et par jour chez les pondeuses naines, mais la taille des oeufs, le pourcentage de gros oeufs et le rendement alimentaire

    E-print Network

    Paris-Sud XI, Université de

    Le taux protéique avait peu d'effet sur le poids corporel et la ponte par poule et par jour chez- mance des naines à celle des non-naines était : pour le poids corporel, 70 p. 100 ; la ponte, 80 p. 100 ce dernier doit être employé à grande échelle commerciale pour la ponte. DONNÉES COMPLÉMENTAIRES SUR

  20. Le processus de commissionnement vu par le maitre d'ouvrage 

    E-print Network

    Moro, M.

    2004-01-01

    co?t pr?visionnel pour inscription au plan national triennal.#0;?#0;? Programme: r?alisation du programme technique: prestations et niveaux de performances ? atteindre.#0;?#0;? Concours: examen des projets par rapport au programme. ?valuations r...;?#0;? Techniques susceptibles d??tre utilis?es : Proposer au programme plusieurs solutions, r?aliser des simulations ? l?APS, la volum?trie ?tant connue, et retenir la solution la plus performante. ? Solution courante : chauffage au gaz et climatisation par groupes...

  1. Nécrolyse épidermique toxique induite par le phénobarbital chez un enfant Rwandais: à propos d'uncas

    PubMed Central

    Kaputu-Kalala-Malu, Célestin; Ntumba-Tshitenge, Olga; Misson, Jean-Paul

    2014-01-01

    Le syndrome de Lyell ou la nécrolyse épidermique toxique (TEN) est une des rares complications majeures du traitement par phénobarbital. Sa prise en charge n'est pas encore codifiée. Il requiert une intervention urgente, lourde et adaptée à chaque patient afin d'en réduire la mortalité. Nous décrivons un cas du syndrome de Lyell survenu une dizaine de jours après initiation du traitement antiépileptique par phénobarbital chez un enfant rwandais de deux ans. La complexité des lésions cutanéomuqueuses et leurs répercussions sur le plan général soulignent l'importance d'une prescription responsable et justifiée des médicaments antiépileptiques. PMID:25396028

  2. Expression of JAM-A, AF-6, PAR-3 and PAR-6 during the assembly and remodeling of RPE tight junctions.

    PubMed

    Luo, Yan; Fukuhara, Masayuki; Weitzman, Matthew; Rizzolo, Lawrence J

    2006-09-19

    The tight junctions of the endothelial and epithelial regions of the blood-brain barrier are regulated by interactions with the neighboring tissue. We examined how the neural retina regulates the assembly of tight junctions in the retinal pigment epithelium (RPE). The proteins JAM-A, AF-6, PAR-3 and PAR-6 have been implicated in the assembly of other epithelial tight junctions. Using chick embryos and primary cell culture, we examined gene expression of these proteins during embryonic development, and whether retinal secretions regulate their expression. Three highly conserved RNA splice sites of AF-6 were identified in chick ocular tissues, but only two were expressed in RPE. JAM-A and AF-6 were expressed at relatively high levels early in development when adherens junctions form, but before tight junctions form. Expression of JAM-A and the AF-6 isoforms actually decreased when tight junctions were forming and expanding. The expression of PAR-3 and PAR-6 was constant. Despite the expression of these proteins in vitro (along with claudins, occludin, ZO-1 and ZO-2), the tight junctional networks that form were discontinuous (Rahner, C., Fukuhara, M., Peng, S., Kojima, S., Rizzolo, L.J., 2004. The apical and basal environments of the retinal pigment epithelium regulate the maturation of tight junctions during development. J. Cell Sci. 117, 3307-3318). The expression of these assembly proteins was unaffected by a retinal conditioned medium that induced the completion of tight junction formation. These data indicate that the early expression of the assembly proteins corresponds to the initial establishment of the adherens and tight junctions, but secretory products of the neural retina must induce the expression of additional proteins to complete the maturation process. PMID:16859655

  3. Communiqu de presse 29 juillet 2013 Remise du Prix W.S. Cooper par l'Ecological Society of America

    E-print Network

    Communiqué de presse ­ 29 juillet 2013 Remise du Prix W.S. Cooper par l'Ecological Society liée aux changements climatiques recevra le 5 août prochain le prestigieux prix William Skinner Cooper par l'Ecological Society of America1 . Le prix W.S. Cooper est décerné pour rendre hommage à une

  4. Action palliative par le convertisseur statique en cas de dfaillance d'un gnrateur PAC modulaire de puissance

    E-print Network

    Paris-Sud XI, Université de

    Action palliative par le convertisseur statique en cas de défaillance d'un générateur PAC modulaire sur les convertisseurs statiques de puissance d'interface et permettant le rétablissement de cette'une énergie embarquée et d'un convertisseur non polluant. La pile à combustible (PàC), alimentée par de l

  5. CONVERTISSEUR TEMPS -AMPLITUDE A POUVOIR DE RSOLUTION LEVE (*) Par S. GORODETZKY, R. RICHERT,R. MANQUENOUILLE et A. KNIPPER,

    E-print Network

    Paris-Sud XI, Université de

    388. CONVERTISSEUR TEMPS - AMPLITUDE A POUVOIR DE RÉSOLUTION ÉLEVÉE (*) Par S. GORODETZKY, R donnons les performances d'un convertisseur temps-amplitude. La courbe de résolution avec les rayonsy du, Principe. - Le schéma d'ensemble est donné par la figure 1, où seul le convertisseur délai-am- plitude est

  6. CONVERTISSEUR TEMPS-AMPLITUDE A TEMPS DE RSOLUTION DE 10-10 SECONDE Par J. SAMUELI et A. SARAZIN,

    E-print Network

    Paris-Sud XI, Université de

    109 A. CONVERTISSEUR TEMPS-AMPLITUDE A TEMPS DE RÉSOLUTION DE 10-10 SECONDE Par J. SAMUELI et A conversion d'intervalles de temps en amplitudes. Sa résolution est de 10-10 seconde. Abstract. 2014 A time par la méthode des temps de vol, nous a conduit à étudier un appareil de conversion d'intervalles de

  7. CROISSANCE DE NANOPARTICULES D'ARGENT PAR INSOLATION LASER ULTRAVIOLETTE CONTINUE DANS DES VERRES SODA-LIME

    E-print Network

    Paris-Sud XI, Université de

    CROISSANCE DE NANOPARTICULES D'ARGENT PAR INSOLATION LASER ULTRAVIOLETTE CONTINUE DANS DES VERRES concentration dans des verres soda-lime par insolation laser continue à 244 nm. L'originalité de cette-CLEFS : nanoparticules ; argent ; verres soda-lime ; coalescence. 1. INTRODUCTION La croissance de NP d'argent peut être

  8. CARACTRISATION PAR A. T. D. DE VERRES DU SYSTME Se-Te-Ge0,5Sb0,5

    E-print Network

    Paris-Sud XI, Université de

    681 CARACTÉRISATION PAR A. T. D. DE VERRES DU SYSTÈME Se-Te-Ge0,5Sb0,5 S. BORDAS, M. D. BARÓ, J on essaye d'étudier les propriétés des verres semiconducteurs est due à leur caractère métastable. Ainsi la verres obtenus par refroidissement du mélange liquide pour différentes conditions (vitesses comprises

  9. Meta-compilation non intrusive du filtrage par contraintes Non intrusive meta-compilation of matching by constraints

    E-print Network

    Boyer, Edmond

    M´eta-compilation non intrusive du filtrage par contraintes Non intrusive meta l'industrie ILOG, INRIA Lorraine, INRIA Rocquencourt inria-00280938,version1-20May2008 #12;MANIFICO ­ M´eta-compilation non intrusive du filtrage par contraintes Historique 31 ao^ut 2004 V 0.9 cr

  10. ATP control of dynamic P1 ParA–DNA interactions: a key role for the nucleoid in plasmid partition

    PubMed Central

    Vecchiarelli, Anthony G; Han, Yong-Woon; Tan, Xin; Mizuuchi, Michiyo; Ghirlando, Rodolfo; Biertümpfel, Christian; Funnell, Barbara E; Mizuuchi, Kiyoshi

    2010-01-01

    P1 ParA is a member of the Walker-type family of partition ATPases involved in the segregation of plasmids and bacterial chromosomes. ATPases of this class interact with DNA non-specifically in vitro and colocalize with the bacterial nucleoid to generate a variety of reported patterns in vivo. Here, we directly visualize ParA binding to DNA using total internal reflection fluorescence microscopy. This activity depends on, and is highly specific for ATP. DNA-binding activity is not coupled to ATP hydrolysis. Rather, ParA undergoes a slow multi-step conformational transition upon ATP binding, which licenses ParA to bind non-specific DNA. The kinetics provide a time-delay switch to allow slow cycling between the DNA binding and non-binding forms of ParA. We propose that this time delay, combined with stimulation of ParA's ATPase activity by ParB bound to the plasmid DNA, generates an uneven distribution of the nucleoid-associated ParA, and provides the motive force for plasmid segregation prior to cell division. PMID:20659294

  11. Principes fondamentaux de la nouvelle politique slave Appel aux peuples slaves par un patriote russe (et variantes)

    E-print Network

    Paris-Sud XI, Université de

    Annexes : Principes fondamentaux de la nouvelle politique slave Appel aux peuples slaves par un patriote russe (et variantes) Appel aux Slaves par un patriote russe La situation en Russie Ma défense halshs-00394269,version1-12May2010 #12;[1] Principes fondamentaux de la nouvelle politique slave1 Après

  12. The Product of par-4, a Gene Induced during Apoptosis, Interacts Selectively with the Atypical Isoforms of Protein Kinase C

    Microsoft Academic Search

    María T Díaz-Meco; María M Municio; Sonia Frutos; Pilar Sanchez; José Lozano; Laura Sanz; Jorge Moscat

    1996-01-01

    The atypical PKCs are involved in a number of important cellular functions, including cell proliferation. We report here that the product of the par-4 gene specifically interacts with the regulatory domains of ?PKC and ?\\/?PKC, which dramatically inhibits their enzymatic activity. This is particularly challenging, because expression of par-4 has been shown to correlate with growth inhibition and apoptosis. Results

  13. The distribution and nature of gonadotropic cells in the rostral pars distalis of the Atlantic salmon, Salmo salar

    E-print Network

    Paris-Sud XI, Université de

    salmon, Salmo salar B. EKENGREN J. PEUTE G. FRIDBERG University of Stockholm, Department of Zoology, Box the gonadotropic (GTH) cells in the rostral pars distalis (RPD) of the pituitary in the Atlantic salmon in phases are generally distributed in the proximal pars distalis. In the sockeye salmon the GTH cells also occur

  14. Simulation de l'volution rcente et future du climat par les modles du CNRM et de l'IPSL

    E-print Network

    Dufresne, Jean-Louis

    bilan est dominé par deux phénomènes, l'absorption du rayonnement solaire (qui apporte de l'énergie) et les échanges par rayonnement infrarouge (qui contrôle les pertes d'énergie vers l'espace) [Bard 2004 De même un changement de l'énergie solaire incidente peut changer le climat, ce qui inquiète Fourier

  15. Immunocytochemical studies on the pituitary pars distalis of the Japanese long-fingered bat, Miniopterus schreibersii fuliginosus

    Microsoft Academic Search

    Shin-ichi Mikami; Shin Chiba; Hitoshi Hojo; Kazuyuki Taniguchi; Kaoru Kubokawa; Susumu Ishii

    1988-01-01

    Immunocytochemical studies were performed to describe the characteristics of cell types and their distribution in the pars distalis of Japanese long-fingered bat, Miniopterus schreibersii fuliginosus, collected at various stages of the reproductive cycle. Six distinct cell types have been identified in the pars distalis by the unlabeled immunoperoxidase technique and by the ABC method. Growth hormone (GH) and prolactin (PRL)

  16. Universit de Montral Rendu d'images en demi-tons par diffusion d'erreur sensible la structure

    E-print Network

    Montréal, Université de

    - ing, ordered dither, direct binary search, and most particularly error diffusion. The meth- odsUniversité de Montréal Rendu d'images en demi-tons par diffusion d'erreur sensible à la structure des arts et des sciences Ce mémoire intitulé: Rendu d'images en demi-tons par diffusion d

  17. Diffusion de l'arsenic dans les structures silicium-sur-isolant obtenues par implantation d'oxygne

    E-print Network

    Boyer, Edmond

    1369 Diffusion de l'arsenic dans les structures silicium-sur-isolant obtenues par implantation d, les auteurs étudient la diffusion de l'arsenic dans les structures Silicium-Sur- Isolant obtenues par Resistance (SR) montrent que l'arsenic est accéléré et que des impuretés inactives s'accumulent dans la

  18. DTERMINATION ET INTERPRTATION DES SPECTRES DE FLUORESCENCE DES GAZ RARES EXCITS PAR UN RAYONNEMENT 03B1

    E-print Network

    Boyer, Edmond

    110. DÉTERMINATION ET INTERPRÉTATION DES SPECTRES DE FLUORESCENCE DES GAZ RARES EXCITÉS PAR UN, on a déterminé les spectres de fluorescence des gaz rares excités par le rayonnement 03B1 de 210Po, la pression of argon, where, in addition, the origin of the emission spectrum is not well known. Finally, in the cases

  19. Characterization of pars intermedia connections in amphibians by biocytin tract tracing and immunofluorescence aided by confocal microscopy

    Microsoft Academic Search

    Koen Jansen; Claudia Fabro; Cristina Artero; Marc Feuilloley; Hubert Vaudry; Aldo Fasolo; Maria F. Franzoni

    1997-01-01

    Biocytin, recently introduced in neuroanatomical studies, was used as a retrograde tract tracer in combination with immunofluorescence in order to analyse the neurochemical characters of some central neuronal projections to the pars intermedia in two amphibian species, the anuran Rana esculenta and the urodele Triturus carnifex. After biocytin insertions in the pars intermedia, neurons became retrogradely labelled in the suprachiasmatic

  20. ramne par des ions appropris la charge sa valeur primitive, le gonflement osmotique rtrograde. Ces faits dclent travers la

    E-print Network

    Paris-Sud XI, Université de

    un tube muni d'une électrode A constituée par un disque d'aluminium ; la seconde électrode B, en'on puisse y introduire un thermo- mètre. Le courant est fourni par une batterie de 3.200 volts en série avec

  1. Fine structure of gonadotrophs and prolactin producing cells in the rat pars distalis in organ culture.

    PubMed Central

    Baker, T G; Young, B A

    1979-01-01

    A technique is described which permits the maintenance for up to 8 days in organ culture of explanted rat pituitary glands. Electron microscope studies showed that the cultured glands (whether treated with gonadotrophin-releasing hormone (GnRH) or not) were almost identical to controls in appearance, although the stellate (follicular) cells seemingly were eliminated in vitro. Gonadotrophs and prolactin-secreting cells in male and female pituitary glands were similar in appearance both in vivo and in vitro to comparable cells described by other authors. There was some evidence for an increase in the secretory activity of these cell types (particularly gonadotrophs) when the explants were treated with GnRH. The present technique thus provides a suitable and valid 'model' with which to study the effects of GnRH and steroid hormones on the release and synthesis of FSH and LH in vitro. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6 Fig. 7 Fig. 8 PMID:395144

  2. Selected basis for PAR reduction in multi-user downlink scenarios using lattice-reduction-aided precoding

    NASA Astrophysics Data System (ADS)

    Siegl, Christian; Fischer, Robert FH

    2011-12-01

    The application of OFDM within a multi-user downlink scenario is considered. Thereby, two problems occur. First, due to OFDM, the transmit signal exhibits a large peak-to-average power ratio (PAR). Second, the multi-user interferences have to be equalized (or precoded) at the transmitter side. In this article, we address combined precoding and PAR reduction. As precoding schemes sorted Tomlinson-Harashima precoding (sTHP) and its lattice-reduction-aided variant (LRA-THP) are considered. In order to reduce the PAR, we review the scheme selected sorting (SLS), which is a combined approach of PAR reduction and precoding with sTHP. Based on this idea, the novel PAR reduction scheme selected basis (SLB) is introduced which combines PAR reduction with the precoding approach LRA-THP. It can be shown that SLB achieves very good PAR reduction performance and hardly influences the error performance. Both schemes, SLB and SLS, are compared with simplified selected mapping (sSLM), the only PAR reduction scheme from the SLM family, which can be applied in multi-user downlink scenarios. The comparison is done on the basis that the respective schemes exhibit the same computational complexity. In terms of PAR reduction performance, it turns out that sSLM outperforms SLS, whereas the performance of sSLM and SLB is similar. Noteworthy, the great benefit of SLB or SLS is that no side information has to be communicated to the receiver as it is necessary with sSLM. Moreover, using SLB, full diversity error rate performance is possible with only low-PAR transmit signals.

  3. Stabilizing a flexible interdomain hinge region harboring the SMB binding site drives uPAR into its closed conformation.

    PubMed

    Zhao, Baoyu; Gandhi, Sonu; Yuan, Cai; Luo, Zhipu; Li, Rui; Gårdsvoll, Henrik; de Lorenzi, Valentina; Sidenius, Nicolai; Huang, Mingdong; Ploug, Michael

    2015-03-27

    The urokinase-type plasminogen activator receptor (uPAR) is a multidomain glycolipid-anchored membrane protein, which facilitates extracellular matrix remodeling by focalizing plasminogen activation to cell surfaces via its high-affinity interaction with uPA. The modular assembly of its three LU (Ly6/uPAR-like) domains is inherently flexible and binding of uPA drives uPAR into its closed conformation, which presents the higher-affinity state for vitronectin thus providing an allosteric regulatory mechanism. Using a new class of epitope-mapped anti-uPAR monoclonal antibodies (mAbs), we now demonstrate that the reciprocal stabilization is indeed also possible. By surface plasmon resonance studies, we show that these mAbs and vitronectin have overlapping binding sites on uPAR and that they share Arg91 as hotspot residue in their binding interfaces. The crystal structure solved for one of these uPAR·mAb complexes at 3.0Å clearly shows that this mAb preselects the closed uPAR conformation with an empty but correctly assembled large hydrophobic binding cavity for uPA. Accordingly, these mAbs inhibit the uPAR-dependent lamellipodia formation and migration on vitronectin-coated matrices irrespective of the conformational status of uPAR and its occupancy with uPA. This is the first study to the best of our knowledge, showing that the dynamic assembly of the three LU domains in uPARwt can be driven toward the closed form by an external ligand, which is not engaging the hydrophobic uPA binding cavity. As this binding interface is also exploited by the somatomedin B domain of vitronectin, therefore, this relationship should be taken into consideration when exploring uPAR-dependent cell adhesion and migration in vitronectin-rich environments. PMID:25659907

  4. A novel repressor, par-4, modulates transcription and growth suppression functions of the Wilms' tumor suppressor WT1.

    PubMed Central

    Johnstone, R W; See, R H; Sells, S F; Wang, J; Muthukkumar, S; Englert, C; Haber, D A; Licht, J D; Sugrue, S P; Roberts, T; Rangnekar, V M; Shi, Y

    1996-01-01

    The tumor suppressor WT1 represses and activates transcription. The loss and/or imbalance of the dual transcriptional activity of WT1 may contribute to Wilms' tumor. In this study, we identified par-4 (for prostate apoptosis response) as a WT1-interacting protein that itself functions as a transcriptional repressor. par-4 contains a putative leucine zipper domain and is specifically upregulated during apoptosis of prostate cells (S. F. Sells, D. P. Wood, Jr., S. S. Joshi-Barve, S. Muthukkumar, R. J. Jacob, S. A. Crist, S. Humphreys, and V. M. Rangnekar, Cell Growth Differ. 5:457-466, 1994). The leucine repeat domain of par-4 was shown to interact with the zinc finger DNA binding domain of WT1. Immunoprecipitation-Western blot (immunoblot) analyses demonstrated in vivo WT1-par-4 interactions. par-4 was ubiquitously expressed, and the protein was found in both the nucleus and the cytoplasm. Functionally, par-4 inhibited transcription activated by WT1, but not by the related protein EGR1. Inhibition of WT1-mediated transcription was dependent on the domain of par-4 that mediates its physical association with WT1. In addition, par-4 augmented WT1-mediated repression, possibly by contributing an additional repression domain. Consistent with these results, par-4 functioned as a transcriptional repressor when brought to a promoter via a heterologous DNA binding domain. Significantly, par-4, but not a mutant unable to interact with WT1, rescued growth suppression caused by WT1. Thus, we identified a novel repressor that modulates transcription as well as growth suppression functions of WT1. PMID:8943350

  5. Activation of PAR2 receptors sensitizes primary afferents and causes leukocyte rolling and adherence in the rat knee joint

    PubMed Central

    Russell, FA; Schuelert, N; Veldhoen, VE; Hollenberg, MD; McDougall, JJ

    2012-01-01

    Background and Purpose The PAR2 receptors are involved in chronic arthritis by mechanisms that are as yet unclear. Here, we examined PAR2 activation in the rat knee joint. Experimental Approach PAR2 in rat knee joint dorsal root ganglia (DRG) cells at L3-L5, retrogradely labelled with Fluoro-gold (FG) were demonstrated immunohistochemically. Electrophysiological recordings from knee joint nerve fibres in urethane anaesthetized Wistar rats assessed the effects of stimulating joint PAR2 with its activating peptide, 2-furoyl-LIGRLO-NH2 (1–100 nmol·100 ?L?1, via close intra-arterial injection). Fibre firing rate was recorded during joint rotations before and 15 min after administration of PAR2 activating peptide or control peptide. Leukocyte kinetics in the synovial vasculature upon PAR2 activation were followed by intravital microscopy for 60 min after perfusion of 2-furoyl-LIGRLO-NH2 or control peptide. Roles for transient receptor potential vanilloid-1 (TRPV1) or neurokinin-1 (NK1) receptors in the PAR2 responses were assessed using the selective antagonists, SB366791 and RP67580 respectively. Key Results PAR2 were expressed in 59 ± 5% of FG-positive DRG cells; 100 nmol 2-furoyl-LIGRLO-NH2 increased joint fibre firing rate during normal and noxious rotation, maximal at 3 min (normal; 110 ± 43%, noxious; 90 ± 31%). 2-Furoyl-LIGRLO-NH2 also significantly increased leukocyte rolling and adhesion over 60 min. All these effects were blocked by pre-treatment with SB366791 and RP67580 (P < 0.05 compared with 2-furoyl-LIGRLO-NH2 alone). Conclusions and Implications PAR2 receptors play an acute inflammatory role in the knee joint via TRPV1- and NK1-dependent mechanisms involving both PAR2-mediated neuronal sensitization and leukocyte trafficking. PMID:22849826

  6. Construction of a pTOC-T vector using GST-ParE toxin for direct cloning and selection of PCR products

    Microsoft Academic Search

    Han Geun Kim; Hye Sun Kim; Hyun Jin Hwang; Sung Kyun Chung; Jung Min Lee; Dae Kyun Chung

    2005-01-01

    Using linker insertion mutations, we determined the most stable region of the parE gene which encodes a toxic protein (ParE) that inhibits growth of Escherichia coli. The toxicity of ParE was sustained until a 144?bp linker was inserted into this region. We have developed a 3' T-overhang vector based on these characteristics of the GST-ParE toxin, and named pTOC-T. Because

  7. LES PRIX TERME DE L'ELECTRICIT SUR LE MARCH BRITANNIQUE, UNE APPROCHE PAR LA COINTGRATION

    E-print Network

    Paris-Sud XI, Université de

    1 LES PRIX À TERME DE L'ELECTRICITÉ SUR LE MARCHÉ BRITANNIQUE, UNE APPROCHE PAR LA COINTÉGRATION lien économique entre le prix de l'électricité et le prix des produits communément appelés commodités dans la mesure où ces dernières sont utilisées pour produire de l'électricité. Leurs prix ont un impact

  8. PRIX DE LA COOPERATION ACADEMIQUE NORD -SUD dcern par l'Universit libre de Bruxelles

    E-print Network

    Cerf, Nicolas

    PRIX DE LA COOPERATION ACADEMIQUE NORD - SUD décerné par l'Université libre de Bruxelles Règlement 2012 Article 1er : Le Prix de la coopération académique Nord-Sud est destiné à récompenser un visibilité de l'ULB en tant qu'acteur pour le développement. Article 2 : Le Prix est décerné, tous les deux

  9. INFLAMMATION DES NUAGES DE POUSSIERES PAR DES ETINCELLES ET DES SURFACES CHAUFFEES

    E-print Network

    Paris-Sud XI, Université de

    2001-74 INFLAMMATION DES NUAGES DE POUSSIERES PAR DES ETINCELLES ET DES SURFACES CHAUFFEES C. PROUST - M. BOUDALAA INERIS-BP 2- F60550 Veraeuil-en-Halatte Résumé. Les trois types de sources d'inflammation utilisé. Pour des délais d'inflammation relativement longs (1 à 2 mn), le paramètre caractéristique de l'inflammation

  10. Amorphisation d'une cible d'AsGa par des ions As

    NASA Astrophysics Data System (ADS)

    Beauvillain, J.; Claverie, A.; Akmoum, K.

    1992-03-01

    The “critical damage energy density” model gives a practical analysis of the amorphous-crystalline ion induced transition. Cross-sectional electron microscopy and related diffraction techniques have been applied to the characterization of arsenide-bombardement induced amorphization of gallium arsenide at room temperature. Combining the experimental measurements of the extension of the amorphous layer for increasing doses with concepts arising from the “critical damage energy density” model leads to E_dc values of about 50 eV mol.^{-1} for 100 ke V arsenide, for the cristalline to amorphous transformation to occur. Le modèle de la densité d'énergie critique donne une analyse pratique de la transition cristal/amorphe induite par implantation ionique. Nous l'avons appliqué au cas d'un cristal d'arséniure de gallium irradié, à température ambiante, par des ions arsenic accélérés sous une tension de 100kV. L'observation des dépôts irradiés en microscopie électronique par la tranche nous a permis de tracer un diagramme d'état expérimental qui permet pour un tel système de prédire les effets d'une implantation. En associant ces résultats à la distribution de la densité d'énergie de dommage donnée par TRIM, nous avons trouvé pour ce système une valeur de la densité d'énergie critique égale à 50eV/mol.

  11. Microanalyse par radiographie X balayage et fluorescence X : une conception nouvelle

    E-print Network

    Paris-Sud XI, Université de

    et un convertisseur. Nous déterminons le choix des paramètres et nous évaluons les performances forme de lame a faces parallèles est place entre le convertisseur qui, comme en emission X [3] assurera a une raie electronique caracteristique du convertisseur excitee par une radia- tion X caracteristique

  12. LES VERRES PHOSPHATES, NOUVEAUX MATRIAUX LASER Par O. K. DEUTSCHBEIN, C. C. PAUTRAT, I. M. SVIRCHEVSKY,

    E-print Network

    Paris-Sud XI, Université de

    29. LES VERRES PHOSPHATES, NOUVEAUX MATÉRIAUX LASER Par O. K. DEUTSCHBEIN, C. C. PAUTRAT, I. M-Issy-les-Moulineaux. Résumé. - Pour cette étude, environ 500 échantillons de verres phosphates dopés au spectres d'absorption et de fluorescence des verres phosphates sont plus étroits que ceux des verres

  13. tude par spectroscopie Mssbauer du fer (III) dans des verres silicats multicomposants d'intrt gologique

    E-print Network

    Boyer, Edmond

    1169- Étude par spectroscopie Mössbauer du fer (III) dans des verres silicatés multicomposants d Môssbauer du Fe (III) dans des verres silicatés synthétiques d'intérêt géologique. La composition chimique de ces verres s'étend d'une composition basique, verres de diopside ou de basalte, à une composition

  14. Echantillonnage anisotropique et rendu par points diffrentiels pour les surfaces implicites

    E-print Network

    Boyer, Edmond

    Kalaiah et Varshney [KV01] originellement développé pour les surfaces paramétriques et les maillages nécessairement de lourdes contraintes de topologie. En 2001, Kalaiah and Varshney [KV01, KV03] proposèrent une première vue. La technique d'échan- tillonnage proposée par Kalaiah et Varshney est basiquement un

  15. A comparative ultrastructural study of the pars tuberalis of various mammals, the chicken and the newt

    Microsoft Academic Search

    Horst-Dieter Dellmann; Marie Elisabeth Stoeckel; Colette Hindelang-Gertner; Aimé Porte; Fred Stutinsky

    1974-01-01

    The pars tuberalis of the rat, mouse, garden door mouse, European hamster, cat, cattle, chicken and newt is composed of two main cell types: specific secretory cells and follicular cells. The specific cells are characterized by comparable morphologic features in the investigated species, despite differences in the diameter of the secretory granulated vesicles; the ultrastructural morphology of these cells is

  16. La fondation Rockefeller et la naissance de l'universalisme philanthropique amricain par Ludovic Tourns

    E-print Network

    Boyer, Edmond

    of Philanthropic Foundations in the Reproduction and Production of Hegemony: The Rockefeller Foundation, Inderjeet Parmar, « To Relate Knowledge and Action: The Impact of the Rockefeller Foundation on Foreign1 La fondation Rockefeller et la naissance de l'universalisme philanthropique américain par Ludovic

  17. Dtermination par traage isotopique de la valeur fertilisante du phosphate alumino-calci-

    E-print Network

    Paris-Sud XI, Université de

    of aluminium-calcium phosphate, diammonium phosphate and rock phosphate. The efficiency of 3 phosphate fertilizers (diammonium phosphate (DAP), aluminium-calcium phosphate and rock phosphate), labelled with P32Détermination par traçage isotopique de la valeur fertilisante du phosphate alumino-calci- que

  18. L'ORGANO-DYNAMISME DE HENRI EY : UNE LECTURE PISTMOLOGIQUE par Djamel Melliti

    E-print Network

    Paris-Sud XI, Université de

    L'ORGANO-DYNAMISME DE HENRI EY : UNE LECTURE ÉPISTÉMOLOGIQUE par Djamel Melliti Psychologue de la manière dont Henri Ey articule ces deux perspectives pour comprendre les phénomènes psychopathologiques sous un angle épistémologique original. Mots-clés : épistémologie ­ Henri Ey ­ neurologie

  19. SUR L'HISTOIRE ET LA THORIE DE LA PILE; PAR M. JULES GAY.

    E-print Network

    Paris-Sud XI, Université de

    et à Humphry Davy, alors âgé de 22 ans. Ces savants s'empressèrent de construire et d'essayer le'Al. Volta à Sir Jos. Banks, président de la Société Royale de Londres, du 20 mars 1800, que furent décrits pour la première fois la construction et les effets de la pile. Cette Lettre ne fut communiquée par Sir

  20. La fracture de Hahn Steinthal traitée par vissage d'Herbert: 3 cas

    PubMed Central

    Bienvenu, Bouhelo-Pam Kevin Parfait; Amine, El Rhazi; Khalid, Chmali; Mohamed, Azarkane; Mohamed, El Idrissi; Mohamed, Shimi; Abdelhalim, El Ibrahimi; Abdelmajid, El Mrini

    2015-01-01

    Les fractures du capitulum sont rares. Leur prise en charge initiale doit être précoce et efficace en raison des risques engendrés sur le coude: rigidité, instabilité, arthrose. De nombreux traitements ont été proposés. Notre étude décrit le vissage par vis d'Herbert pratiqué chez trois patientes recensées entre 2012 et 2013. Elles ont été inclues selon les critères de traumatisme du coude avec douleur exquise externe avec un trait de fracture radiologique frontal du condyle huméral externe emportant la joue externe de la trochlée. Le diagnostic a été orienté par l'examen clinique et confirmé à la radiographie de face, de profil et des ¾ internes. Les lésions ont été classées selon Bryan et Morrey. Les patients ont été opérés en urgence par abord postéro-latéral de Kocher, réduction à ciel ouvert puis stabilisation par vis de Herbert enfouies. Le recul moyen a été de un an. La récupération fonctionnelle totale moyenne a été de 3,6 mois. L’évaluation fonctionnelle a été jugée excellente selon le score MEPI (Mayo Elbow Performance Index) pour les trois patients. Il n'y a pas eu de démontage de matériel. La consolidation osseuse moyenne a été de 2,6 mois. PMID:26015850

  1. CHIMIE et POTIONS MAGIQUES Lecture de la srie Harry Potter par un chimiste.

    E-print Network

    Paris-Sud XI, Université de

    CHIMIE et POTIONS MAGIQUES Lecture de la série « Harry Potter » par un chimiste. LA CHIMIE DE...) pour 1 Enquête en cours sur Harry Potter et le système éducatif. -1- hal-00322790,version1-18Sep2008 Manuscrit auteur, publié dans "Harry Potter; la crise dans le miroir., Nice : France (2008)" #12;leur

  2. 04/10/2004 ; LPS Orsay Etude par RMN et SR des

    E-print Network

    04/10/2004 ; LPS Orsay Etude par RMN et µSR des composés antiferromagnétiques fortement frustrés à Physique des Solides Laboratoire Léon Brillouin #12;04/10/2004 ; LPS Orsay Systèmes antiferromagnétiques ? Spins Heisenberg AF AF AF AF AF AF Spins Heisenberg #12;04/10/2004 ; LPS Orsay Réseaux de taille

  3. Essai d'amlioration de la rsistance la scheresse du tournesol (Helianthus annuus) par croisement interspci-

    E-print Network

    Paris-Sud XI, Université de

    Essai d'amélioration de la résistance à la sécheresse du tournesol (Helianthus annuus) par Helianthus annuus x H. argophyllus a été effectué en 1977 dans le but d'obtenir de nouveaux cultivars de argophyllus. Methodology and first results. An interspecific cross between Helianthus annuus and Helianthus

  4. Le produit et le rayonnement de l'uranium X1 Par F. SODDY

    E-print Network

    Paris-Sud XI, Université de

    53 Le produit et le rayonnement de l'uranium X1 Par F. SODDY [Laboratoire de chimie-physique de l probablement deux pro- duits à rayons '1., intermédiaires entre l'uranium X et te radium. Boltwood a montre différence de 12 unités entre le poids atomique de l'uranium et celui dut radium. Cette différence correspond

  5. EFFETS DES FAIBLES IRRADIATIONS SUR L'URANIUM Par Y. QUR,

    E-print Network

    Boyer, Edmond

    489. EFFETS DES FAIBLES IRRADIATIONS SUR L'URANIUM Par Y. QUÉRÉ, Centre d'Études Nucléaires de fragments de fission dans l'uranium, on présente quelques résultats de mesure de longueur et de résistivité modifiés. Abstract. 2014 After recalling Brinkman's model for the action of fission fragments on uranium

  6. Fine structure of the chromophobe in the pars distalis of the common snapping turtle, Chelydra serpentina

    Microsoft Academic Search

    Michael T. Tseng; Chester L. Yntema

    1976-01-01

    Chromophobes of the pars distalis in young Chelydra serpentina have sparse cytoplasm with no specific granules; however, many cytoplasmic filaments are present. The chromophobes are connected to the other cell types by desmosomes, while different types of junctional specializations occur between adjacent chromophobes. Cytoplasmic filaments traverse the cytoplasm in a random manner and terminate on both the junctional complexes and

  7. Segmentation des signaux SEEG par dcomposition en paquets de Malvar frquentiels

    E-print Network

    Paris-Sud XI, Université de

    Segmentation des signaux SEEG par décomposition en paquets de Malvar fréquentiels E. HITTI, F'une décomposition en Paquets de Malvar Fréquentiels. Elle est appliquée à la détection de changements dans des packets of Malvar. It is applied to the detection of changes in intracerebral EEG signals (SEEG) recorded

  8. A-21 Personnel Activity Reports (PAR) Certification of Effort For Salaries and Wages

    E-print Network

    Tam, Tin-Yau

    A-21 Personnel Activity Reports (PAR) Certification of Effort For Salaries and Wages Charged must be signed by someone who has first-hand knowledge that the salaries or wages charged between the "% of Monthly Salary" and the "Estimate of Actual % of Time," the salary distribution should

  9. Ocanographie / Oceanography Bathymay : la structure sous-marine de Mayotte rvle par l'imagerie multifaisceaux

    E-print Network

    Boyer, Edmond

    Océanographie / Oceanography 1/24 Bathymay : la structure sous-marine de Mayotte révélée par l 338 (2006) 1240-1249" DOI : 10.1016/j.crte.2006.07.010 #12;Océanographie / Oceanography 2/24 Résumé La hal-00870100,version1-28Oct2013 #12;Océanographie / Oceanography 3/24 Abridged English version

  10. PARAMAGNTISME DES FERRITES DE FER, DE COBALT, DE NICKEL Par M. FALLOT et P. MARONI.

    E-print Network

    Paris-Sud XI, Université de

    PARAMAGNÉTISME DES FERRITES DE FER, DE COBALT, DE NICKEL Par M. FALLOT et P. MARONI. Sommaire. - Les auteurs étudient Je paramagnétisme des ferrites de fer, cobalt, nickel, avec une précision accrue température exploré est insuffisant; sur le ferrite de cobalt, on ne possède aucun résultat. - Nos expériences

  11. DPLACEMENT ISOTOPIQUE DANS LE SPECTRE DE L'INDIUM I (1) Par DEREK A. JACKSON.

    E-print Network

    Paris-Sud XI, Université de

    459. DÃ?PLACEMENT ISOTOPIQUE DANS LE SPECTRE DE L'INDIUM I (1) Par DEREK A. JACKSON. Laboratoire A. - La structure hyperfine dans le spectre d'arc, et dans le premier spectre d'6tincelle de 1'indium'intensit6 (1'indium naturel contient seu- lement 4,1 % de 113In) conduisant a des raies de 113In trop

  12. PROPRITS OPTIQUES DES COUCHES MINCES D'INDIUM Par M. VAN DE VOORDE et A. JONES,

    E-print Network

    Boyer, Edmond

    543. PROPRIÉTÉS OPTIQUES DES COUCHES MINCES D'INDIUM Par M. VAN DE VOORDE et A. JONES, Centre de Physique Nucléaire, Université de Louvain. Résumé. 2014 Les propriétés optiques des couches minces d'indium. 2014 The optical properties of thin layers of indium have been studied by Burtin [1], Motulevich

  13. IP over ATM: Classical IP, NHRP, LANE, MPOA, PAR and I-PNNI

    E-print Network

    Jain, Raj

    IP over ATM: Classical IP, NHRP, LANE, MPOA, PAR and I-PNNI Jun Xu Due to the popularity and dominance of the TCP/IP, the success of ATM as a data networking technology lies largely in its support of IP on top of it. This report surveys the proposed approaches of transporing IP traffic

  14. Digestion des glucides de la levure d'alcanes par le veau prruminant

    E-print Network

    Paris-Sud XI, Université de

    Digestion des glucides de la levure d'alcanes par le veau préruminant J. M. BESLE, Bernadette LASSALAS, P. THIVEND Laboratoire de la Digestion des Ruminanfs, 1.N.R.A., Theix, 63110 Beaumont France. Summary. Digestion of alkane yeast carbohydrates by the preruminant calf. Four 15-day old preruminant

  15. Prediction de series chronologiques par selection de mod`eles et randomisation.

    E-print Network

    Paris-Sud XI, Université de

    Pr´ediction de s´eries chronologiques par s´election de mod`eles et randomisation. Pierre Alquier Lattre De Tassigny 75775 PARIS CEDEX 16 FRANCE Abstract Observing a stationary time series, we present in this presentation new two-steps proce- dures for predicting the next value of the time series. Following machine

  16. courspublics2013/2014 Les cours publics proposs depuis plusieurs annes par

    E-print Network

    Halazonetis, Thanos

    .unige.ch/communication/agenda #12;FACULTÉ DES SCIENCES 6-9 Physique Biologie Astronomie Sciences de la terre et environnement Chimie Allemand Anglais Russe Etudes est-asiatiques Philosophie Histoire Histoire de l'art Musicologie Etudes'infiniment petit: l'Univers comme laboratoire pour la physique fondamentale par le Dr Martin Kunz 6 novembre 2013 L'astronomie

  17. Utilisation du glucose et du cellobiose par trois souches de Fibrobacter succinogenes

    E-print Network

    Paris-Sud XI, Université de

    Utilisation du glucose et du cellobiose par trois souches de Fibrobacter succinogenes G Gaudet KJ compared with respect to their growth using glucose andlor cellobiose as the carbon and energy substrate(s) and their capacities to de- grade cellulose. The growth rate of F succinogenes strain S85 was the same using glucose

  18. Interactions gniteur x population des partenaires Il. Dtection par des expriences de slection

    E-print Network

    Boyer, Edmond

    Interactions géniteur x population des partenaires Il. Détection par des expériences de sélection J'une population Pa et la population de leurs partenaires (Pa ou Pb). La première partie consiste à établir un de sélection intra-population conduites sur 2 popula- tions utilisées en croisement et expériences de

  19. FACTEURS FAVORISANT LA PRODUCTION D'ACIDE PNICILLIQUE PAR PENICILLIUM VERRUCOSUM VAR. CYCLOPIUM

    E-print Network

    Paris-Sud XI, Université de

    , 180, chemin de Tournefeuille, 3130i0 Toulouse, France Summary ENHANCEMENT FACTORS OF PENICILLIC ACID PRODUCTION BY PENlClLLlUM VERRUCO- SUM VAR. CYCLOPlUM IN FOODSTUFFS. - Main parameters of penicillic acid (PFACTEURS FAVORISANT LA PRODUCTION D'ACIDE PÉNICILLIQUE PAR PENICILLIUM VERRUCOSUM VAR. CYCLOPIUM

  20. Prvision de la consommation d'lectricit par correction itrative du biais

    E-print Network

    Paris-Sud XI, Université de

    ) RTE - Département Méthodes et Appui, vincent.le...eux@rte-france.com (4) IRMAR UMR 6625 - Université charge. Les prévisions utilisées quotidiennement par RTE sont issues d'un modèle alliant une régression révèle performante. Abstract Réseau de Transport d'Electricité (RTE), in charge of operating the French