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Sample records for partner nuclear profile

  1. Sister Lab Program Prospective Partner Nuclear Profile: Indonesia

    SciTech Connect

    Bissani, M; Tyson, S

    2006-12-14

    Indonesia has participated in cooperative technical programs with the IAEA since 1957, and has cooperated with regional partners in all of the traditional areas where nuclear science is employed: in medicine, public health (such as insect control and eradication programs), agriculture (e.g. development of improved varieties of rice), and the gas and oil industries. Recently, Indonesia has contributed significantly to the Reduced Enrichment Research and Training Reactor (RERTR) Program by conducting experiments to confirm the feasibility of Mo-99 production using high-density low enriched uranium (LEU) fuel, a primary goal of the RERTR Program. Indonesia's first research reactor, the TRIGA Mark II at Bandung, began operation in 1964 at 250 kW and was subsequently upgraded in 1971 to 1 MW and further upgraded in 2000 to 2 MW. This reactor was joined by another TRIGA Mark II, the 100-kW Kartini-PPNY at Yogyakarta, in 1979, and by the 30-MW G.A. Siwabessy multipurpose reactor in Serpong, which achieved criticality in July 1983. A 10-MW radioisotope production reactor, to be called the RPI-10, also was proposed for construction at Serpong in the late 1990s, but the project apparently was not carried out. In the five decades since its nuclear research program began, Indonesia has trained a cadre of scientific and technical staff who not only operate and conduct research with the current facilities, but also represent the nucleus of a skilled labor pool to support development of a nuclear power program. Although Indonesia's previous on-again, off-again consideration of nuclear power has not gotten very far in the past, it now appears that Indonesia again is giving serious consideration to beginning a national nuclear energy program. In June 2006, Research and Technology Minister Kusmayanto Kadiman said that his ministry was currently putting the necessary procedures in place to speed up the project to acquire a nuclear power plant, indicating that, ''We will need around

  2. Sister Lab Program Prospective Partner Nuclear Profile: Malaysia

    SciTech Connect

    Bissani, M; Tyson, S

    2006-12-14

    The Malaysian Deputy Prime Minister Tun Dr Ismail Abdul Rahman suggested in the early 1970s that Malaysia should have a role in the development of nuclear science and technology for peaceful purposes. Accordingly, the Center for the Application of Nuclear Energy (CRANE) was established, with a focus on the development of a scientific and technical pool critical to a national nuclear power program. The Malaysian Cabinet next established the Tun Ismail Atomic Research Center (TIARC) under the Ministry of Science, Technology and the Environment on 19 September 1972, at a site in Bangi, about 35 km south of Kuala Lampur. On 28 June 1982, the PUSPATI reactor, a 1-MW TRIGA MK-II research reactor, first reached criticality. On 10 August 1994, TIARC was officially renamed as the Malaysian Institute for Nuclear Technology Research (MINT). In addition to radioisotope production and neutron radiography conducted at the PUSPATI research reactor, MINT also supports numerous programs employing nuclear technology for medicine, agriculture and industry, and has been involved in both bilateral and multilateral technical cooperation to extend its capabilities. As an energy exporting country, Malaysia has felt little incentive to develop a nuclear energy program, and high level opposition within the government discouraged it further. A recent statement by Malaysia's Science, Technology and Innovation Minister supported this view, indicating that only a near-catastrophic jump in world oil prices might change the government's view. However, the rate at which Malaysia is using its natural gas and oil reserves is expected to force it to reassess the role of nuclear energy in the near future. In addition, the government does intend to construct a radioactive waste repository to dispose of naturally occurring radioactive materials (extracted during tin mining, in particular). Also, Malaysia's growing economy could encourage expansion in Malaysia's existing nuclear-applications programs

  3. Sister Lab Program Prospective Partner Nuclear Profile: Vietnam

    SciTech Connect

    Bissani, M; Tyson, S

    2006-12-14

    Vietnam's nuclear program began in the 1960s with the installation at Dalat of a 250 kW TRIGA Mk-II research reactor under the U.S. Atoms for Peace Program. The reactor was shut down and its core removed only a few years later, and the nuclear research program was suspended until after the end of the civil war in the late 1970s. The Soviet Union assisted Vietnam in restoring the Dalat reactor to an operational status in 1984, trained a cadre of scientific and technical staff in its operation, and contributed to the development of nuclear science for the medical and agricultural sectors. In the agricultural area in particular, Vietnamese experts have been very successful in developing mutant strains of rice, and continue to work with the IAEA to yield strains that have a shorter growing period, increased resistance to disease, and other desirable characteristics. Rice has always been the main crop in Vietnam, but technical cooperation with the IAEA and other states has enabled the country to become one of the top rice producers in the world, exporting much of its annual crop to over two dozen countries annually. More recently, Vietnam's government has shown increasing interest in developing a civil nuclear program to supplement its fossil fuel and other energy resources. Projections from a variety of open sources, ranging from the IAEA, the U.S. Department of Energy's Energy Information Administration (EIA), the Vietnamese government, energy corporations, and think tanks all predict a massive increase in energy consumption--especially electricity--within Vietnam and the region as a whole. This growth in consumption will require a corresponding increase in energy production, which in Vietnam is currently satisfied mainly by fossil fuels (coal) and renewable energy (hydropower and biomass); Vietnam has a refining capacity of about 800 barrels/day. Most of its crude oil is exported to generate export income, and is not used to generate electricity. Although Vietnam is

  4. "Are we Facebook official?" Implications of dating partners' Facebook use and profiles for intimate relationship satisfaction.

    PubMed

    Papp, Lauren M; Danielewicz, Jennifer; Cayemberg, Crystal

    2012-02-01

    Extending previous research on positive and negative correlates of Facebook use for individuals' outcomes, this study examined male and female dating partners' (n=58 couples) Facebook use and portrayals of their intimate relationship on the Facebook profile. Confirming hypotheses from compatibility theories of mate selection, partners demonstrated similar Facebook intensity (e.g., usage, connection to Facebook), and were highly likely to portray their relationship on their Facebook profiles in similar ways (i.e., display partnered status and show their partner in profile picture). These Facebook profile choices played a role in the overall functioning of the relationship, with males' indications of a partnered status linked with higher levels of their own and their partners' (marginal) relationship satisfaction, and females' displays of their partner in their profile picture linked with higher levels of their own and their partners' relationship satisfaction. Finally, male and female reports of having had disagreements over the Facebook relationship status was associated with lower level of females' but not males' relationship satisfaction, after accounting for global verbal conflict. Thus, the findings point to the unique contribution of Facebook disagreements to intimate relationship functioning. Results from this study encourage continued examination of technology use and behaviors in contexts of intimate relationships. PMID:21988733

  5. Anger Problem Profiles among Partner Violent Men: Differences in Clinical Presentation and Treatment Outcome

    ERIC Educational Resources Information Center

    Murphy, Christopher M.; Taft, Casey T.; Eckhardt, Christopher I.

    2007-01-01

    Cluster analysis of 139 partner violent men's self-reports on the State-Trait Anger Expression Inventory identified profiles reflecting pathological anger (PA), low anger control (LAC), and normal anger (NA). The PA group self-reported higher pretreatment partner abuse, interpersonal dysfunction, distress, and substance abuse and had lower…

  6. Police Attitudes toward Policing Partner Violence against Women: Do They Correspond to Different Psychosocial Profiles?

    ERIC Educational Resources Information Center

    Gracia, Enrique; Garcia, Fernando; Lila, Marisol

    2011-01-01

    This study analyzed whether police attitudes toward policing partner violence against women corresponded with different psychosocial profiles. Two attitudes toward policing partner violence were considered--one reflecting a general preference for a conditional law enforcement (depending on the willingness of the victim to press charges against the…

  7. Perceived Partner Responsiveness Predicts Diurnal Cortisol Profiles 10 Years Later

    PubMed Central

    Slatcher, Richard B.; Selcuk, Emre; Ong, Anthony D.

    2015-01-01

    Several decades of research have demonstrated that marital relationships have a powerful influence on physical health. However, surprisingly little is known about how marriage affects health—both in terms of psychological processes and biological ones. We investigated the associations between perceived partner responsiveness—the extent to which people feel understood, cared for and appreciated by their romantic partner—and diurnal cortisol over a 10-year period in a large sample of married and cohabitating couples in the U.S. Partner responsiveness predicted higher wakeup cortisol values and steeper (“healthier”) cortisol slopes at the 10-year follow-up, and these associations remained strong after controlling for demographic factors, depressive symptoms, agreeableness, and other positive and negative relationship factors. Further, declines in negative affect over the 10-year period mediated the prospective association between responsiveness and cortisol slope. These findings suggest that diurnal cortisol may be a key biological pathway through which social relationships impact long-term health. PMID:26015413

  8. Partners.

    PubMed

    Westover, P F

    1986-01-01

    The Salt Lake Clinic's problem was one of balance. Although the organizational values of the clinic were well developed, the organizational structure was not. The board of directors historically was accountable to its partners or shareholders, but the competitive, consumer-oriented environment also called for recognition of community, business, and consumer interest. To achieve a more balanced approach to clinic governance, a lay advisory board was appointed, made up of members active in civic affairs who each had a unique contribution to make and represented a business, community, or consumer perspective. PMID:10278455

  9. Latent profiles of perceived time adequacy for paid work, parenting, and partner roles.

    PubMed

    Lee, Soomi; Almeida, David M; Davis, Kelly D; King, Rosalind B; Hammer, Leslie B; Kelly, Erin L

    2015-10-01

    This study examined feelings of having enough time (i.e., perceived time adequacy) in a sample of employed parents (N = 880) in information technology and extended-care industries. Adapting a person-centered latent profile approach, we identified 3 profiles of perceived time adequacy for paid work, parenting, and partner roles: family time protected, family time sacrificed, and time balanced. Drawing upon the conservation of resources theory (Hobfòll, 1989), we examined the associations of stressors and resources with the time adequacy profiles. Parents in the family time sacrificed profile were more likely to be younger, women, have younger children, work in the extended-care industry, and have nonstandard work schedules compared to those in the family time protected profile. Results from multinomial logistic regression analyses revealed that, with the time balanced profile as the reference group, having fewer stressors and more resources in the family context (less parent-child conflict and more partner support), work context (longer company tenure, higher schedule control and job satisfaction), and work-family interface (lower work-to-family conflict) was linked to a higher probability of membership in the family time protected profile. By contrast, having more stressors and fewer resources, in the forms of less partner support and higher work-to-family conflict, predicted a higher likelihood of being in the family time sacrificed profile. Our findings suggest that low work-to-family conflict is the most critical predictor of membership in the family time protected profile, whereas lack of partner support is the most important factor to be included in the family time sacrificed profile. PMID:26075739

  10. Distinguishing Risk Profiles among Parent-Only, Partner-Only, and Dually-Perpetrating Physical Aggressors

    PubMed Central

    Smith Slep, Amy M.; O’Leary, Susan G.

    2009-01-01

    This study addressed whether and how men and women who are not physically aggressive, only physically aggressive against their children or their partners, or aggressive against both family members differ in their risk profiles. Risk factors that were unrelated to the partner or parenting role (e.g., impulsivity) and that were specific to one of these roles (e.g., negative parenting attributions, negative partner attributions) were examined using profile analysis. Dually aggressive men and women had the highest overall risk across all types of risk factors; non-aggressive men and women had consistently low risk. Individuals who were aggressive toward only their partners or only their children had distinct risk profiles, with highest levels of risk on the role-specific variable sets. With the exception of parent-only aggressive men, singly aggressive individuals’ risk levels were significantly lower on role-independent and the unrelated role-specific risk than they were on the role-related risk factors. These results suggest theories of partner and parent aggression might gain precision if co-occurrence status were specifically taken into account. PMID:19803606

  11. Oxidative stress-induced assembly of PML nuclear bodies controls sumoylation of partner proteins.

    PubMed

    Sahin, Umut; Ferhi, Omar; Jeanne, Marion; Benhenda, Shirine; Berthier, Caroline; Jollivet, Florence; Niwa-Kawakita, Michiko; Faklaris, Orestis; Setterblad, Niclas; de Thé, Hugues; Lallemand-Breitenbach, Valérie

    2014-03-17

    The promyelocytic leukemia (PML) protein organizes PML nuclear bodies (NBs), which are stress-responsive domains where many partner proteins accumulate. Here, we clarify the basis for NB formation and identify stress-induced partner sumoylation as the primary NB function. NB nucleation does not rely primarily on intermolecular interactions between the PML SUMO-interacting motif (SIM) and SUMO, but instead results from oxidation-mediated PML multimerization. Oxidized PML spherical meshes recruit UBC9, which enhances PML sumoylation, allow partner recruitment through SIM interactions, and ultimately enhance partner sumoylation. Intermolecular SUMO-SIM interactions then enforce partner sequestration within the NB inner core. Accordingly, oxidative stress enhances NB formation and global sumoylation in vivo. Some NB-associated sumoylated partners also become polyubiquitinated by RNF4, precipitating their proteasomal degradation. As several partners are protein-modifying enzymes, NBs could act as sensors that facilitate and confer oxidative stress sensitivity not only to sumoylation but also to other post-translational modifications, thereby explaining alterations of stress response upon PML or NB loss. PMID:24637324

  12. Elucidating Posttraumatic Stress Symptom Profiles and Their Correlates Among Women Experiencing Bidirectional Intimate Partner Violence

    PubMed Central

    Hellmuth, Julianne C.; Jaquier, Véronique; Swan, Suzanne C.; Sullivan, Tami P.

    2014-01-01

    Objective This study employed latent class analysis to identify profiles of women experiencing intimate partner violence (IPV) based on the severity of posttraumatic stress disorder (PTSD) symptoms. Method Self-report data from a sample of 369 women experiencing bidirectional IPV was used. Results A 3-class solution comprising low, moderate, and high PTSD severity profiles best fit the data. Profiles were differentially related to whether IPV victimization was considered traumatic (PTSD criterion A); whether functioning was impaired as a result of PTSD symptoms (PTSD criterion F); whether the woman met full diagnostic criteria for PTSD; depression symptom severity; and severity of psychological, physical, and sexual IPV victimization and use of IPV. An extremely high percentage of women in the high (96%) and moderate (88%) severity classes experienced functional impairment, although many did not meet full diagnostic criteria for PTSD. Conclusions Findings support the need for interventions individually tailored to one’s treatment needs based on the nature of one’s traumatic stressor and the impact of PTSD on daily functioning. PMID:24752965

  13. In Situ Detection of Interactions Between Nuclear Envelope Proteins and Partners.

    PubMed

    Barateau, Alice; Buendia, Brigitte

    2016-01-01

    Proximity ligation assay (PLA) appears as a quick and easy technique to visualize within fixed cells the occurrence and in situ distribution of protein complexes. PLA has been validated to detect protein-protein interactions within the nuclear compartment. Here, we describe a protocol which allows the detection of interactions between A-type nuclear lamins and either LEM-domain proteins (such as emerin, integrated within the inner nuclear membrane, and LAP2α which accumulates within the nucleoplasm) or gene regulatory factors (e.g., the transcription factor SREBP1). The distinct amounts and patterns of PLA signals obtained for various complexes highlight the pertinence of using PLA to reveal in situ where and to which extent nuclear envelope proteins bind specific partners. PMID:27147040

  14. Oxygen depth profiling by nuclear resonant scattering

    SciTech Connect

    Gibson, G. T.; Sheu, W. J.; Glass, G. A.; Wang, Y. Q.

    1999-06-10

    Nuclear resonance scattering (NRS) {sup 16}O({alpha},{alpha}){sup 16}O at 3.045 MeV ({gamma}=10 keV) has been used for oxygen depth profiling in various thin oxide films. There are two ways by which the oxygen concentration versus depth profile can be obtained from the experimental data: energy spectrum simulation or yield distribution analysis. Energy spectrum simulation is done using the standard RBS software/Rutherford Universal Manipulation Program (RUMP) where only one spectrum is usually needed from the measurement. Yield distribution analysis is accomplished by using a custom developed software/Resonance Analysis Program (RAP) and involves a series of spectra obtained by stepping up the beam energy above the resonance energy. This article aims at comparing the fundamentals of both methods and also discussing their advantages and disadvantages in terms of the data acquisition and the post data analysis. A thermally grown thick SiO{sub 2} film and a thin titanium oxide film grown by corona point discharge were examined.

  15. Oxygen depth profiling by nuclear resonant scattering

    SciTech Connect

    Gibson, G.T.; Sheu, W.J.; Glass, G.A. Wang, Y.Q.

    1999-06-01

    Nuclear resonance scattering (NRS) {sup 16}O({alpha},{alpha}){sup 16}O at 3.045 MeV ({Gamma}=10&hthinsp;keV) has been used for oxygen depth profiling in various thin oxide films. There are two ways by which the oxygen concentration versus depth profile can be obtained from the experimental data: energy spectrum simulation or yield distribution analysis. Energy spectrum simulation is done using the standard RBS software/Rutherford Universal Manipulation Program (RUMP) where only one spectrum is usually needed from the measurement. Yield distribution analysis is accomplished by using a custom developed software/Resonance Analysis Program (RAP) and involves a series of spectra obtained by stepping up the beam energy above the resonance energy. This article aims at comparing the fundamentals of both methods and also discussing their advantages and disadvantages in terms of the data acquisition and the post data analysis. A thermally grown thick SiO{sub 2} film and a thin titanium oxide film grown by corona point discharge were examined. {copyright} {ital 1999 American Institute of Physics.}

  16. A profile of Michigan independent inventors and partnering activity: 1991 survey. Final report

    SciTech Connect

    Eldersveld, B.B.

    1991-12-01

    The Inventors` Council of Michigan created a survey to investigate the use of partnering among independent inventors. A packet consisting of the survey, a cover letter, an informational brochure and a postpaid return envelope was mailed up to 2500 independent inventors selected from the organization`s data base. There were 460 total responses. Of these, the 296 received before the preselected cut-off date were analyzed. The data from these included in the report. Further interviews were conducted with five inventors who had commercialized their inventions with a partner. These case studies led to the conclusion that inventors need to find partners. Although low numbers of inventors actually use partners, the need was expressed for people who can provide skills that inventors lack. These needs include marketing expertise, manufacturing facilities and assistance with financing. Inventors need education and contacts in order to bring their products to market successfully, thereby providing jobs and economic development. Governments should encourage this activity.

  17. A profile of Michigan independent inventors and partnering activity: 1991 survey

    SciTech Connect

    Eldersveld, B.B.

    1991-12-01

    The Inventors' Council of Michigan created a survey to investigate the use of partnering among independent inventors. A packet consisting of the survey, a cover letter, an informational brochure and a postpaid return envelope was mailed up to 2500 independent inventors selected from the organization's data base. There were 460 total responses. Of these, the 296 received before the preselected cut-off date were analyzed. The data from these included in the report. Further interviews were conducted with five inventors who had commercialized their inventions with a partner. These case studies led to the conclusion that inventors need to find partners. Although low numbers of inventors actually use partners, the need was expressed for people who can provide skills that inventors lack. These needs include marketing expertise, manufacturing facilities and assistance with financing. Inventors need education and contacts in order to bring their products to market successfully, thereby providing jobs and economic development. Governments should encourage this activity.

  18. Personality Profiles of Intimate Partner Violence Offenders with and without PTSD

    ERIC Educational Resources Information Center

    Hoyt, Tim; Wray, Alisha M.; Wiggins, Kathryn T.; Gerstle, Melissa; Maclean, Peggy C.

    2012-01-01

    Intimate partner violence (IPV) is a serious forensic and clinical problem throughout the United States. Research aimed at defining and differentiating subgroups of IPV offenders using standardized personality instruments may eventually help with matching treatments to specific individuals to reduce recidivism. The current study used a convenience…

  19. Factors Discriminating among Profiles of Resilience and Psychopathology in Children Exposed to Intimate Partner Violence (IPV)

    ERIC Educational Resources Information Center

    Graham-Bermann, Sandra A.; Gruber, Gabrielle; Howell, Kathryn H.; Girz, Laura

    2009-01-01

    Objective: To evaluate the social and emotional adjustment of 219 children in families with varying levels of intimate partner violence (IPV) using a model of risk and protection. To explore factors that differentiate children with poor adjustment from those with resilience. Methodology: Mothers who experienced IPV in the past year and their…

  20. Bile acids inhibit duodenal secretin expression via orphan nuclear receptor small heterodimer partner (SHP).

    PubMed

    Lam, Ian P Y; Lee, Leo T O; Choi, Hueng-Sik; Alpini, Gianfranco; Chow, Billy K C

    2009-07-01

    Small heterodimer partner (SHP) is an orphan nuclear receptor in which gene expression can be upregulated by bile acids. It regulates its target genes by repressing the transcriptional activities of other nuclear receptors including NeuroD, which has been shown to regulate secretin gene expression. Here, we evaluated the regulation on duodenal secretin gene expression by SHP and selected bile acids, cholic acid (CA) and chenodeoxycholic acid (CDCA). In vitro treatment of CDCA or fexaramine elevated the SHP transcript level and occupancy on secretin promoter. The increase in the SHP level, induced by bile acid treatment or overexpression, reduced secretin gene expression, whereas this gene inhibitory effect was reversed by silencing of endogenous SHP. In in vivo studies, double-immunofluorescence staining demonstrated the coexpression of secretin and SHP in mouse duodenum. Feeding mice with 1% CA-enriched rodent chow resulted in upregulation of SHP and a concomitant decrease in secretin transcript and protein levels in duodenum compared with the control group fed with normal chow. A diet enriched with 5% cholestyramine led to a decrease in SHP level and a corresponding increase in secretin expression. Overall, this study showed that bile acids via SHP inhibit duodenal secretin gene expression. Because secretin is a key hormone that stimulates bile flow in cholangiocytes, this pathway thus provides a novel means to modulate secretin-stimulated choleresis in response to intraduodenal bile acids. PMID:19372104

  1. The orphan nuclear receptor small heterodimer partner mediates male infertility induced by diethylstilbestrol in mice

    PubMed Central

    Volle, David H.; Decourteix, Mélanie; Garo, Erwan; McNeilly, Judy; Fenichel, Patrick; Auwerx, Johan; McNeilly, Alan S.; Schoonjans, Kristina; Benahmed, Mohamed

    2009-01-01

    Studies in rodents have shown that male sexual function can be disrupted by fetal or neonatal administration of compounds that alter endocrine homeostasis, such as the synthetic nonsteroidal estrogen diethylstilbestrol (DES). Although the molecular basis for this effect remains unknown, estrogen receptors likely play a critical role in mediating DES-induced infertility. Recently, we showed that the orphan nuclear receptor small heterodimer partner (Nr0b2), which is both a target gene and a transcriptional repressor of estrogen receptors, controls testicular function by regulating germ cell entry into meiosis and testosterone synthesis. We therefore hypothesized that some of the harmful effects of DES on testes could be mediated through Nr0b2. Here, we present data demonstrating that Nr0b2 deficiency protected mice against the negative effects of DES on testis development and function. During postnatal development, Nr0b2-null mice were resistant to DES-mediated inhibition of germ cell differentiation, which may be the result of interference by Nr0b2 with retinoid signals that control meiosis. Adult Nr0b2-null male mice were also protected against the effects of DES; however, we suggest that this phenomenon was due to the removal of the repressive effects of Nr0b2 on steroidogenesis. Together, these data demonstrate that Nr0b2 plays a critical role in the pathophysiological changes induced by DES in the mouse testis. PMID:19884658

  2. Noninvasive nuclear magnetic resonance profiling of painting layers

    NASA Astrophysics Data System (ADS)

    Presciutti, Federica; Perlo, Juan; Casanova, Federico; Glöggler, Stefan; Miliani, Costanza; Blümich, Bernhard; Brunetti, Brunetto Giovanni; Sgamellotti, Antonio

    2008-07-01

    In this work we demonstrate the potential of single-sided nuclear magnetic resonance (NMR) sensors to access deeper layers of paintings noninvasively by means of high-resolution depth profiles spanning several millimeters. The performance of the sensor in resolving painting structures was tested on models for which excellent agreement with microscopy techniques was obtained. The depth profiling NMR technique was used in situ to investigate old master paintings. The observation of differences in NMR relaxation times of tempera binders from these paintings and from artificially aged panels raises the possibility to differentiate between original and recently restored areas.

  3. A New STAT3-binding Partner, ARL3, Enhances the Phosphorylation and Nuclear Accumulation of STAT3.

    PubMed

    Togi, Sumihito; Muromoto, Ryuta; Hirashima, Koki; Kitai, Yuichi; Okayama, Taichiro; Ikeda, Osamu; Matsumoto, Naoki; Kon, Shigeyuki; Sekine, Yuichi; Oritani, Kenji; Matsuda, Tadashi

    2016-05-20

    Signal transducer and activator of transcription 3 (STAT3) is involved in cell proliferation, differentiation, and cell survival during immune responses, hematopoiesis, neurogenesis, and other biological processes. STAT3 activity is regulated by a variety of mechanisms, including phosphorylation and nuclear translocation. To clarify the molecular mechanisms underlying the regulation of STAT3 activity, we performed yeast two-hybrid screening. We identified ARL3 (ADP-ribosylation factor-like 3) as a novel STAT3-binding partner. ARL3 recognizes the DNA-binding domain as well as the C-terminal region of STAT3 in vivo, and their binding was the strongest when both proteins were activated. Importantly, small interfering RNA-mediated reduction of endogenous ARL3 expression decreased IL-6-induced tyrosine phosphorylation, nuclear accumulation, and transcriptional activity of STAT3. These results indicate that ARL3 interacts with STAT3 and regulates the transcriptional activation of STAT3 by influencing its nuclear accumulation of STAT3. PMID:27048653

  4. Global partnering related to nuclear materials safeguards and security - A pragmatic approach to international safeguards work

    SciTech Connect

    Stanford, Dennis

    2007-07-01

    This paper documents issues Nuclear Fuel Services, Inc. has addressed in the performance of international work to safeguards and security work. It begins with a description of the package we put together for a sample proposal for the Global Threat Reduction Initiative, for which we were ranked number one for technical approach and cost, and concludes with a discussion of approaches that we have taken to performing this work, including issues related to performing the work as part of a team. The primary focus is on communication, workforce, equipment, and coordination issues. Finally, the paper documents the rules that we use to assure the work is performed safely and successfully. (author)

  5. Orphan nuclear receptor small heterodimer partner inhibits transforming growth factor-beta signaling by repressing SMAD3 transactivation.

    PubMed

    Suh, Ji Ho; Huang, Jiansheng; Park, Yun-Yong; Seong, Hyun-A; Kim, Dongwook; Shong, Minho; Ha, Hyunjung; Lee, In-Kyu; Lee, Keesook; Wang, Li; Choi, Hueng-Sik

    2006-12-22

    Orphan nuclear receptor small heterodimer partner (SHP) is an atypical member of the nuclear receptor superfamily; SHP regulates the nuclear receptor-mediated transcription of target genes but lacks a conventional DNA binding domain. In this study, we demonstrate that SHP represses transforming growth factor-beta (TGF-beta)-induced gene expression through a direct interaction with Smad, a transducer of TGF-beta signaling. Transient transfection studies demonstrate that SHP represses Smad3-induced transcription. In vivo and in vitro protein interaction assays revealed that SHP directly interacts with Smad2 and Smad3 but not with Smad4. Mapping of domains mediating the interaction between SHP and Smad3 showed that the entire N-terminal domain (1-159 amino acids) of SHP and the linker domain of Smad3 are involved in this interaction. In vitro glutathione S-transferase pulldown competition experiments revealed the SHP-mediated repression of Smad3 transactivation through competition with its co-activator p300. SHP also inhibits the activation of endogenous TGF-beta-responsive gene promoters, the p21, Smad7, and plasminogen activator inhibitor-1 (PAI-1) promoters. Moreover, adenovirus-mediated overexpression of SHP decreases PAI-1 mRNA levels, and down-regulation of SHP by a small interfering RNA increases both the transactivation of Smad3 and the PAI-1 mRNA levels. Finally, the PAI-1 gene is expressed in SHP(-/-) mouse hepatocytes at a higher level than in normal hepatocytes. Taken together, these data indicate that SHP is a novel co-regulator of Smad3, and this study provides new insights into regulation of TGF-beta signaling. PMID:17074765

  6. A parameterization of nuclear track profiles in CR-39 detector

    NASA Astrophysics Data System (ADS)

    Azooz, A. A.; Al-Nia'emi, S. H.; Al-Jubbori, M. A.

    2012-11-01

    on any theory of liability, whether in contract, strict liability, or tort (including negligence or otherwise) arising in any way out of the use of this software, even if advised of the possibility of such damage. No. of lines in distributed program, including test data, etc.: 15598 No. of bytes in distributed program, including test data, etc.: 3933244 Distribution format: tar.gz Programming language: MATLAB. Computer: Any Desktop or Laptop. Operating system: Windows 1998 or above (with MATLAB R13 or above installed). RAM: 512 Megabytes or higher Classification: 17.5. Nature of problem: A new semispherical parameterization of charged particle tracks in CR-39 SSNTD is carried out in a previous paper. This parameterization is developed here into a MATLAB based software to calculate the track length and track profile for any proton or alpha particle energy or etching time. This software is intended to compete with the TRACK_TEST [1] and TRACK_VISION [2] software currently in use by all people working in the field of SSNTD. Solution method: Based on fitting of experimental results of protons and alpha particles track lengths for various energies and etching times to a new semispherical formula with four free fitting parameters, the best set of energy independent parameters were found. These parameters are introduced into the software and the software is programmed to solve the set of equations to calculate the track depth, track etching rate as a function of both time and residual range for particles of normal and oblique incidence, the track longitudinal profile at both normal and oblique incidence, and the three dimensional track profile at normal incidence. Running time: 1-8 s on Pentium (4) 2 GHz CPU, 3 GB of RAM depending on the etching time value References: [1] ADWT_v1_0 Track_Test Computer program TRACK_TEST for calculating parameters and plotting profiles for etch pits in nuclear track materials. D. Nikezic, K.N. Yu Comput. Phys. Commun. 174(2006)160 [2] AEAF

  7. Profiles of Intimate Partner Violence Victimization, Substance Misuse, and Depression Among Female Caregivers Involved with Child Protective Services

    PubMed Central

    Hellmuth, Julianne C.; Sullivan, Tami P.; Connell, Christian M.

    2014-01-01

    Intimate partner violence (IPV) victimization, substance misuse, and depression are highly prevalent among female caregivers involved with child protective services (CPS). Understanding heterogeneity in the occurrence of these problems is essential to improving pathways to intervention for women in this population. Latent class analysis was employed to determine whether there exist homogeneous subgroups of female caregivers who experience different patterns of IPV victimization, substance misuse, and depression. A restricted three-class solution best fit the data, suggesting high risk, moderate risk, and no risk subgroups. A substantial number of female caregivers comprise the high-risk subgroup (33%) in which caregivers reported very high rates of IPV victimization, substance misuse, and depression. Only a very small proportion comprised the no-risk subgroup (9%). Findings emphasize the heterogeneity among subgroups of female caregivers based on these risk factors, which may have implications for practitioners, such as CPS caseworkers, and researchers alike. PMID:26617433

  8. A parameterization of nuclear track profiles in CR-39 detector

    NASA Astrophysics Data System (ADS)

    Azooz, A. A.; Al-Nia'emi, S. H.; Al-Jubbori, M. A.

    2012-11-01

    on any theory of liability, whether in contract, strict liability, or tort (including negligence or otherwise) arising in any way out of the use of this software, even if advised of the possibility of such damage. No. of lines in distributed program, including test data, etc.: 15598 No. of bytes in distributed program, including test data, etc.: 3933244 Distribution format: tar.gz Programming language: MATLAB. Computer: Any Desktop or Laptop. Operating system: Windows 1998 or above (with MATLAB R13 or above installed). RAM: 512 Megabytes or higher Classification: 17.5. Nature of problem: A new semispherical parameterization of charged particle tracks in CR-39 SSNTD is carried out in a previous paper. This parameterization is developed here into a MATLAB based software to calculate the track length and track profile for any proton or alpha particle energy or etching time. This software is intended to compete with the TRACK_TEST [1] and TRACK_VISION [2] software currently in use by all people working in the field of SSNTD. Solution method: Based on fitting of experimental results of protons and alpha particles track lengths for various energies and etching times to a new semispherical formula with four free fitting parameters, the best set of energy independent parameters were found. These parameters are introduced into the software and the software is programmed to solve the set of equations to calculate the track depth, track etching rate as a function of both time and residual range for particles of normal and oblique incidence, the track longitudinal profile at both normal and oblique incidence, and the three dimensional track profile at normal incidence. Running time: 1-8 s on Pentium (4) 2 GHz CPU, 3 GB of RAM depending on the etching time value References: [1] ADWT_v1_0 Track_Test Computer program TRACK_TEST for calculating parameters and plotting profiles for etch pits in nuclear track materials. D. Nikezic, K.N. Yu Comput. Phys. Commun. 174(2006)160 [2] AEAF

  9. Repression of the nuclear receptor small heterodimer partner by steatotic drugs and in advanced nonalcoholic fatty liver disease.

    PubMed

    Benet, Marta; Guzmán, Carla; Pisonero-Vaquero, Sandra; García-Mediavilla, M Victoria; Sánchez-Campos, Sonia; Martínez-Chantar, M Luz; Donato, M Teresa; Castell, José Vicente; Jover, Ramiro

    2015-04-01

    The small heterodimer partner (SHP) (NR0B2) is an atypical nuclear receptor that lacks a DNA-binding domain. It interacts with and inhibits many transcription factors, affecting key metabolic processes, including bile acid, cholesterol, fatty acid, and drug metabolism. Our aim was to determine the influence of steatotic drugs and nonalcoholic fatty liver disease (NAFLD) on SHP expression and investigate the potential mechanisms. SHP was found to be repressed by steatotic drugs (valproate, doxycycline, tetracycline, and cyclosporin A) in cultured hepatic cells and the livers of different animal models of NAFLD: iatrogenic (tetracycline-treated rats), genetic (glycine N-methyltransferase-deficient mice), and nutritional (mice fed a methionine- and choline-deficient diet). Among the different transcription factors investigated, CCAAT-enhancer-binding protein α (C/EBPα) showed the strongest dominant-repressive effect on SHP expression in HepG2 and human hepatocytes. Reporter assays revealed that the inhibitory effect of C/EBPα and steatotic drugs colocalize between -340 and -509 base pair of the SHP promoter, and mutation of a predicted C/EBPα response element at -473 base pair abolished SHP repression by both C/EBPα and drugs. Moreover, inhibition of major stress signaling pathways demonstrated that the mitogen-activated protein kinase kinase 1/2 pathway activates, while the phosphatidylinositol 3 kinase pathway represses SHP in a C/EBP-dependent manner. We conclude that SHP is downregulated by several steatotic drugs and in advanced NAFLD. These conditions can activate signals that target C/EBPα and consequently repress SHP, thus favoring the progression and severity of NAFLD. PMID:25576488

  10. Women’s Stress, Depression, and Relationship Adjustment Profiles as They Relate to Intimate Partner Violence and Mental Health During Pregnancy and Postpartum

    PubMed Central

    Flanagan, Julianne C.; Gordon, Kristina Coop; Moore, Todd M.; Stuart, Gregory L.

    2014-01-01

    Objective This study applied latent class analysis to examine whether homogeneous subgroups of women emerged based on their self-reported stress, depression, and relationship adjustment during pregnancy. We also examined whether women in different groups experienced different intimate partner violence (IPV) and mental health symptoms during pregnancy and postpartum. Method 180 women completed assessments during the first 18 weeks of pregnancy and 122 completed follow-up assessments six weeks postpartum. Results A two-class solution best fit the data. One group reported higher mean stress and depression and poorer relationship adjustment compared to the other group. The high severity class reported more psychological IPV victimization and perpetration and more physical IPV victimization during pregnancy compared to the low severity class. Membership in the high severity class was associated with higher postpartum depression. Conclusions Findings highlight the associations between different profiles of mental and relational health during pregnancy and postpartum. Future studies should explore the utility of dyadic interventions aimed at reducing stress, depression, and IPV, and improving relationship adjustment as a means to improve women’s health during pregnancy and postpartum. These findings also highlight the potential utility of applying person-centered analytic approaches to the study of women’s and couples’ health during this time period. PMID:25642352

  11. Nuclear microprobe analysis of lead profile in crocodile bones

    NASA Astrophysics Data System (ADS)

    Orlic, I.; Siegele, R.; Hammerton, K.; Jeffree, R. A.; Cohen, D. D.

    2003-09-01

    Elevated concentrations of lead were found in Australian free ranging saltwater crocodile ( Crocodylus porosus) bone and flesh. Lead shots were found as potential source of lead in these animals. ANSTO's heavy ion nuclear microprobe was used to measure the distribution of Pb in a number of bones and osteoderms. The aim was to find out if elevated Pb concentration remains in growth rings and if the concentration is correlated with the blood levels recorded at the time. Results of our study show a very distinct distribution of accumulated Pb in bones and osteoderms as well as good correlation with the level of lead concentration in blood. To investigate influence of ion species on detection limits measurements of the same sample were performed by using 3 MeV protons, 9 MeV He ions and 20 MeV carbon ions. Peak to background ratios, detection limits and the overall 'quality' of obtained spectra are compared and discussed.

  12. Civic Partners.

    ERIC Educational Resources Information Center

    Pew Partnership for Civic Change, Charlottesville, VA.

    This issue of "Civic Partners" is a call to action on behalf of American's cities. The issue opens with John W. Gardner's discussion of the "responsibles" whose vision and energy sustain communities. He stresses that all of us are "responsibles." Among the many tasks that face those responsible for urban improvement is the teaching of conflict…

  13. Nup2 requires a highly divergent partner, NupA, to fulfill functions at nuclear pore complexes and the mitotic chromatin region

    PubMed Central

    Markossian, Sarine; Suresh, Subbulakshmi; Osmani, Aysha H.; Osmani, Stephen A.

    2015-01-01

    Chromatin and nuclear pore complexes (NPCs) undergo dramatic changes during mitosis, which in vertebrates and Aspergillus nidulans involves movement of Nup2 from NPCs to the chromatin region to fulfill unknown functions. This transition is shown to require the Cdk1 mitotic kinase and be promoted prematurely by ectopic expression of the NIMA kinase. Nup2 localizes with a copurifying partner termed NupA, a highly divergent yet essential NPC protein. NupA and Nup2 locate throughout the chromatin region during prophase but during anaphase move to surround segregating DNA. NupA function is shown to involve targeting Nup2 to its interphase and mitotic locations. Deletion of either Nup2 or NupA causes identical mitotic defects that initiate a spindle assembly checkpoint (SAC)–dependent mitotic delay and also cause defects in karyokinesis. These mitotic problems are not caused by overall defects in mitotic NPC disassembly–reassembly or general nuclear import. However, without Nup2 or NupA, although the SAC protein Mad1 locates to its mitotic locations, it fails to locate to NPCs normally in G1 after mitosis. Collectively the study provides new insight into the roles of Nup2 and NupA during mitosis and in a surveillance mechanism that regulates nucleokinesis when mitotic defects occur after SAC fulfillment. PMID:25540430

  14. Subcellular RNA profiling links splicing and nuclear DICER1 to alternative cleavage and polyadenylation.

    PubMed

    Neve, Jonathan; Burger, Kaspar; Li, Wencheng; Hoque, Mainul; Patel, Radhika; Tian, Bin; Gullerova, Monika; Furger, Andre

    2016-01-01

    Alternative cleavage and polyadenylation (APA) plays a crucial role in the regulation of gene expression across eukaryotes. Although APA is extensively studied, its regulation within cellular compartments and its physiological impact remains largely enigmatic. Here, we used a rigorous subcellular fractionation approach to compare APA profiles of cytoplasmic and nuclear RNA fractions from human cell lines. This approach allowed us to extract APA isoforms that are subjected to differential regulation and provided us with a platform to interrogate the molecular regulatory pathways that shape APA profiles in different subcellular locations. Here, we show that APA isoforms with shorter 3' UTRs tend to be overrepresented in the cytoplasm and appear to be cell-type-specific events. Nuclear retention of longer APA isoforms occurs and is partly a result of incomplete splicing contributing to the observed cytoplasmic bias of transcripts with shorter 3' UTRs. We demonstrate that the endoribonuclease III, DICER1, contributes to the establishment of subcellular APA profiles not only by expected cytoplasmic miRNA-mediated destabilization of APA mRNA isoforms, but also by affecting polyadenylation site choice. PMID:26546131

  15. Subcellular RNA profiling links splicing and nuclear DICER1 to alternative cleavage and polyadenylation

    PubMed Central

    Neve, Jonathan; Burger, Kaspar; Li, Wencheng; Hoque, Mainul; Patel, Radhika; Tian, Bin; Gullerova, Monika; Furger, Andre

    2016-01-01

    Alternative cleavage and polyadenylation (APA) plays a crucial role in the regulation of gene expression across eukaryotes. Although APA is extensively studied, its regulation within cellular compartments and its physiological impact remains largely enigmatic. Here, we used a rigorous subcellular fractionation approach to compare APA profiles of cytoplasmic and nuclear RNA fractions from human cell lines. This approach allowed us to extract APA isoforms that are subjected to differential regulation and provided us with a platform to interrogate the molecular regulatory pathways that shape APA profiles in different subcellular locations. Here, we show that APA isoforms with shorter 3′ UTRs tend to be overrepresented in the cytoplasm and appear to be cell-type–specific events. Nuclear retention of longer APA isoforms occurs and is partly a result of incomplete splicing contributing to the observed cytoplasmic bias of transcripts with shorter 3′ UTRs. We demonstrate that the endoribonuclease III, DICER1, contributes to the establishment of subcellular APA profiles not only by expected cytoplasmic miRNA-mediated destabilization of APA mRNA isoforms, but also by affecting polyadenylation site choice. PMID:26546131

  16. A new approach to nuclear fuel safeguard enhancement through radionuclide profiling

    NASA Astrophysics Data System (ADS)

    Peterson, Aaron Dawon

    The United States has led the effort to promote peaceful use of nuclear power amongst states actively utilizing it as well as those looking to deploy the technology in the near future. With the attraction being demonstrated by various countries towards nuclear power comes the concern that a nation may have military aspirations for the use of nuclear energy. The International Atomic Energy Agency (IAEA) has established nuclear safeguard protocols and procedures to mitigate nuclear proliferation. The work herein proposed a strategy to further enhance existing safeguard protocols by considering safeguard in nuclear fuel design. The strategy involved the use of radionuclides to profile nuclear fuels. Six radionuclides were selected as identifier materials. The decay and transmutation of these radionuclides were analyzed in reactor operation environment. MCNPX was used to simulate a reactor core. The perturbation in reactivity of the core due to the loading of the radionuclides was insignificant. The maximum positive and negative reactivity change induced was at day 1900 with a value of 0.00185 +/- 0.00256 and at day 2000 with -0.00441 +/- 0.00249, respectively. The mass of the radionuclides were practically unaffected by transmutation in the core; the change in radionuclide inventory was dominated by natural decay. The maximum material lost due to transmutation was 1.17% in Eu154. Extraneous signals from fission products identical to the radionuclide compromised the identifier signals. Eu154 saw a maximum intensity change at EOC and 30 days post-irradiation of 1260% and 4545%, respectively. Cs137 saw a minimum change of 12% and 89%, respectively. Mitigation of the extraneous signals is cardinal to the success of the proposed strategy. The predictability of natural decay provides a basis for the characterization of the signals from the radionuclide.

  17. Profiles.

    ERIC Educational Resources Information Center

    School Arts, 1979

    1979-01-01

    Profiles seven Black, Native American, and Chicano artists and art teachers: Hale A. Woodruff, Allan Houser, Luis Jimenez, Betrand D. Phillips, James E. Pate, I, and Fernando Navarro. This article is part of a theme issue on multicultural art. (SJL)

  18. Benefits of utilizing CellProfiler as a characterization tool for U-10Mo nuclear fuel

    SciTech Connect

    Collette, R.; Douglas, J.; Patterson, L.; Bahun, G.; King, J.; Keiser, D.; Schulthess, J.

    2015-05-01

    Automated image processing techniques have the potential to aid in the performance evaluation of nuclear fuels by eliminating judgment calls that may vary from person-to-person or sample-to-sample. Analysis of in-core fuel performance is required for design and safety evaluations related to almost every aspect of the nuclear fuel cycle. This study presents a methodology for assessing the quality of uranium-molybdenum fuel images and describes image analysis routines designed for the characterization of several important microstructural properties. The analyses are performed in CellProfiler, an open-source program designed to enable biologists without training in computer vision or programming to automatically extract cellular measurements from large image sets. The quality metric scores an image based on three parameters: the illumination gradient across the image, the overall focus of the image, and the fraction of the image that contains scratches. The metric presents the user with the ability to ‘pass’ or ‘fail’ an image based on a reproducible quality score. Passable images may then be characterized through a separate CellProfiler pipeline, which enlists a variety of common image analysis techniques. The results demonstrate the ability to reliably pass or fail images based on the illumination, focus, and scratch fraction of the image, followed by automatic extraction of morphological data with respect to fission gas voids, interaction layers, and grain boundaries.

  19. Benefits of utilizing CellProfiler as a characterization tool for U-10Mo nuclear fuel

    DOE PAGESBeta

    Collette, R.; Douglas, J.; Patterson, L.; Bahun, G.; King, J.; Keiser, D.; Schulthess, J.

    2015-05-01

    Automated image processing techniques have the potential to aid in the performance evaluation of nuclear fuels by eliminating judgment calls that may vary from person-to-person or sample-to-sample. Analysis of in-core fuel performance is required for design and safety evaluations related to almost every aspect of the nuclear fuel cycle. This study presents a methodology for assessing the quality of uranium-molybdenum fuel images and describes image analysis routines designed for the characterization of several important microstructural properties. The analyses are performed in CellProfiler, an open-source program designed to enable biologists without training in computer vision or programming to automatically extract cellularmore » measurements from large image sets. The quality metric scores an image based on three parameters: the illumination gradient across the image, the overall focus of the image, and the fraction of the image that contains scratches. The metric presents the user with the ability to ‘pass’ or ‘fail’ an image based on a reproducible quality score. Passable images may then be characterized through a separate CellProfiler pipeline, which enlists a variety of common image analysis techniques. The results demonstrate the ability to reliably pass or fail images based on the illumination, focus, and scratch fraction of the image, followed by automatic extraction of morphological data with respect to fission gas voids, interaction layers, and grain boundaries.« less

  20. Temperature Profile in Fuel and Tie-Tubes for Nuclear Thermal Propulsion Systems

    SciTech Connect

    Vishal Patel

    2015-02-01

    A finite element method to calculate temperature profiles in heterogeneous geometries of tie-tube moderated LEU nuclear thermal propulsion systems and HEU designs with tie-tubes is developed and implemented in MATLAB. This new method is compared to previous methods to demonstrate shortcomings in those methods. Typical methods to analyze peak fuel centerline temperature in hexagonal geometries rely on spatial homogenization to derive an analytical expression. These methods are not applicable to cores with tie-tube elements because conduction to tie-tubes cannot be accurately modeled with the homogenized models. The fuel centerline temperature directly impacts safety and performance so it must be predicted carefully. The temperature profile in tie-tubes is also important when high temperatures are expected in the fuel because conduction to the tie-tubes may cause melting in tie-tubes, which may set maximum allowable performance. Estimations of maximum tie-tube temperature can be found from equivalent tube methods, however this method tends to be approximate and overly conservative. A finite element model of heat conduction on a unit cell can model spatial dependence and non-linear conductivity for fuel and tie-tube systems allowing for higher design fidelity of Nuclear Thermal Propulsion.

  1. Benefits of utilizing CellProfiler as a characterization tool for U–10Mo nuclear fuel

    SciTech Connect

    Collette, R.; Douglas, J.; Patterson, L.; Bahun, G.; King, J.; Keiser, D.; Schulthess, J.

    2015-07-15

    Automated image processing techniques have the potential to aid in the performance evaluation of nuclear fuels by eliminating judgment calls that may vary from person-to-person or sample-to-sample. Analysis of in-core fuel performance is required for design and safety evaluations related to almost every aspect of the nuclear fuel cycle. This study presents a methodology for assessing the quality of uranium–molybdenum fuel images and describes image analysis routines designed for the characterization of several important microstructural properties. The analyses are performed in CellProfiler, an open-source program designed to enable biologists without training in computer vision or programming to automatically extract cellular measurements from large image sets. The quality metric scores an image based on three parameters: the illumination gradient across the image, the overall focus of the image, and the fraction of the image that contains scratches. The metric presents the user with the ability to ‘pass’ or ‘fail’ an image based on a reproducible quality score. Passable images may then be characterized through a separate CellProfiler pipeline, which enlists a variety of common image analysis techniques. The results demonstrate the ability to reliably pass or fail images based on the illumination, focus, and scratch fraction of the image, followed by automatic extraction of morphological data with respect to fission gas voids, interaction layers, and grain boundaries. - Graphical abstract: Display Omitted - Highlights: • A technique is developed to score U–10Mo FIB-SEM image quality using CellProfiler. • The pass/fail metric is based on image illumination, focus, and area scratched. • Automated image analysis is performed in pipeline fashion to characterize images. • Fission gas void, interaction layer, and grain boundary coverage data is extracted. • Preliminary characterization results demonstrate consistency of the algorithm.

  2. Lipoprotein Particle Profiles by Nuclear Magnetic Resonance Spectroscopy in Medically-Underserved HIV-Infected Persons

    PubMed Central

    Swanson, Barbara; Sha, Beverly E.; Keithley, Joyce K.; Fogg, Louis; Nerad, Judith; Novak, Richard; Adeyemi, Oluwatoyin

    2009-01-01

    Background HIV infection is associated with dyslipidemia and increased risk for cardiovascular events. Few studies have described lipid status in medically-underserved, HIV-infected ethnic minorities, a group that is characterized by health disparities. Objective The objective was to characterize the lipid profile of a medically-underserved, largely ethnic minority sample of HIV-infected persons using standard lipid panels and nuclear magnetic resonance (NMR) -derived lipoprotein particle profiles. Methods Participants were recruited from a randomized controlled trial of a dietary supplement to manage HIV-related dyslipidemia (N=132). At the initial screening visit, sociodemographic, clinical, and behavioral data were collected, and fasting peripheral venous blood specimens were obtained and lipid status was analyzed using the standard lipid panel and the NMR-derived lipoprotein particle profile. Results Using NMR-derived LDL particle cutoffs, a higher percentage of participants was outside the target range (50%) than when standard LDL cholesterol NCEP cutoffs were used (24%). Antiretroviral therapy, especially protease inhibitor-containing regimens, was associated with higher LDL particle concentration. Conclusion Substantial numbers of medically-underserved, asymptomatic HIV-infected minorities may be at increased risk for CHD based on NMR-derived lipoprotein values. PMID:20161509

  3. Modulation of expression of the nuclear receptor NR0B2 (small heterodimer partner 1) and its impact on proliferation of renal carcinoma cells

    PubMed Central

    Prestin, Katharina; Olbert, Maria; Hussner, Janine; Isenegger, Tamara L; Gliesche, Daniel G; Böttcher, Kerstin; Zimmermann, Uwe; Meyer zu Schwabedissen, Henriette E

    2016-01-01

    Mammalian nuclear receptors (NRs) are transcription factors regulating the expression of target genes that play an important role in drug metabolism, transport, and cellular signaling pathways. The orphan and structurally unique receptor small heterodimer partner 1 (syn NR0B2) is not only known for its modulation of drug response, but has also been reported to be involved in hepatocellular carcinogenesis. Indeed, previous studies show that NR0B2 is downregulated in human hepatocellular carcinoma, suggesting that NR0B2 acts as a tumor suppressor via inhibition of cellular growth and activation of apoptosis in this tumor entity. The aim of our study was to elucidate whether NR0B2 may also play a role in other tumor entities. Comparing NR0B2 expression in renal cell carcinoma and adjacent nonmalignant transformed tissue revealed significant downregulation in vivo. Additionally, the impact of heterologous expression of NR0B2 on cell cycle progression and proliferation in cells of renal origin was characterized. Monitoring fluorescence intensity of resazurin turnover in RCC-EW cells revealed no significant differences in metabolic activity in the presence of NR0B2. However, there was a significant decrease of cellular proliferation in cells overexpressing this NR, and NR0B2 was more efficient than currently used antiproliferative agents. Furthermore, flow cytometry analysis showed that heterologous overexpression of NR0B2 significantly reduced the amount of cells passing the G1 phase, while on the other hand, more cells in S/G2 phase were detected. Taken together, our data suggest that downregulation of NR0B2 may also play a role in renal cell carcinoma development and progression. PMID:27540300

  4. Modulation of expression of the nuclear receptor NR0B2 (small heterodimer partner 1) and its impact on proliferation of renal carcinoma cells.

    PubMed

    Prestin, Katharina; Olbert, Maria; Hussner, Janine; Isenegger, Tamara L; Gliesche, Daniel G; Böttcher, Kerstin; Zimmermann, Uwe; Meyer Zu Schwabedissen, Henriette E

    2016-01-01

    Mammalian nuclear receptors (NRs) are transcription factors regulating the expression of target genes that play an important role in drug metabolism, transport, and cellular signaling pathways. The orphan and structurally unique receptor small heterodimer partner 1 (syn NR0B2) is not only known for its modulation of drug response, but has also been reported to be involved in hepatocellular carcinogenesis. Indeed, previous studies show that NR0B2 is downregulated in human hepatocellular carcinoma, suggesting that NR0B2 acts as a tumor suppressor via inhibition of cellular growth and activation of apoptosis in this tumor entity. The aim of our study was to elucidate whether NR0B2 may also play a role in other tumor entities. Comparing NR0B2 expression in renal cell carcinoma and adjacent nonmalignant transformed tissue revealed significant downregulation in vivo. Additionally, the impact of heterologous expression of NR0B2 on cell cycle progression and proliferation in cells of renal origin was characterized. Monitoring fluorescence intensity of resazurin turnover in RCC-EW cells revealed no significant differences in metabolic activity in the presence of NR0B2. However, there was a significant decrease of cellular proliferation in cells overexpressing this NR, and NR0B2 was more efficient than currently used antiproliferative agents. Furthermore, flow cytometry analysis showed that heterologous overexpression of NR0B2 significantly reduced the amount of cells passing the G1 phase, while on the other hand, more cells in S/G2 phase were detected. Taken together, our data suggest that downregulation of NR0B2 may also play a role in renal cell carcinoma development and progression. PMID:27540300

  5. Miniaturized Nuclear Magnetic Resonance Platform for Detection and Profiling of Circulating Tumor Cells

    PubMed Central

    Castro, Cesar M.; Ghazani, Arezou A.; Chung, Jaehoon; Shao, Huilin; Issadore, David; Yoon, Tae-Jong; Weissleder, Ralph; Lee, Hakho

    2013-01-01

    Accurate detection and profiling of circulating tumor cells (CTCs) is a highly sought after technology to improve cancer management. Such “liquid biopsies” could offer a non-invasive, repeatable window into each patient’s tumor, facilitating early cancer diagnosis and treatment monitoring. The rarity of CTCs, approximated at 1 CTC for every billion peripheral blood cells, however, poses significant challenges to sensitive and reliable detection. We have recently developed a new biosensor platform, namely a micro-nuclear magnetic resonance (µNMR). Through the synergistic integration of microfabrication, nanosensors, and novel chemistries, the µNMR platform offers high detection sensitivity and point-of-care operation, overcoming technical barriers in CTC research. We herein review the µNMR technology with emphasis on its application on CTC detection. Recent advances in the sensing technology will be summarized, followed by the description on the dynamic interplay between preclinical and clinical CTC studies. PMID:23835814

  6. Nuclear magnetic resonance-based study reveals the metabolomics profile of nasopharyngeal carcinoma.

    PubMed

    Wang, Y; Luo, X; Zhang, G H; Li, S L

    2016-01-01

    Proton nuclear magnetic resonance ([(1)H]-NMR) spectroscopy has been used to investigate metabolites in serum and several types of tissue. We used NMR spectroscopy to explore the differential metabolic profiles in serum from nasopharyngeal carcinoma (NPC) patients. Moreover, metabolites with potential as biomarkers for identifying NPC patients were primarily identified. Serum samples were collected from 40 enrolled participants comprising 20 healthy subjects and 20 NPC patients. Samples were analyzed using a 600-MHz NMR spectrometer. The [(1)H]-NMR spectra were further analyzed with partial least squares-discriminant analysis for screening differential metabolites. NMR spectroscopy identified a total of eight metabolites that were present at different levels when the sera of NPC patients were compared with those of healthy individuals. Methionine, taurine (P < 0.05), and choline-like metabolites (P < 0.05) were mostly elevated in the sera of NPC patients. In contrast, the levels of lipids (P < 0.01), isoleucine (P < 0.05), unsaturated lipids (P < 0.01), trimethylamine oxidase (P < 0.05), and carbohydrates (P < 0.05) were lower in the sera of the NPC patients than in the healthy controls. We explored the differential metabolic profiles in sera from NPC patients. [(1)H]-NMR spectroscopy can be used to identify specific metabolites, and is capable of distinguishing between NPC patients and healthy individuals. PMID:27323073

  7. Kinetic Profile of Amyloid Formation in the Presence of an Aromatic Inhibitor by Nuclear Magnetic Resonance

    PubMed Central

    2012-01-01

    The self-assembly of amyloid proteins into β-sheet rich assemblies is associated with human amyloidoses including Alzheimer's disease, Parkinson's disease, and type 2 diabetes. An attractive therapeutic strategy therefore is to develop small molecules that would inhibit protein self-assembly. Natural polyphenols are potential inhibitors of β-sheet formation. How these compounds affect the kinetics of self-assembly studied by thioflavin T (ThT) fluorescence is not understood primarily because their presence interferes with ThT fluorescence. Here, we show that by plotting peak intensities from nuclear magnetic resonance (NMR) against incubation time, kinetic profiles in the presence of the polyphenol can be obtained from which kinetic parameters of self-assembly can be easily determined. In applying this technique to the self-assembly of the islet amyloid polypeptide in the presence of curcumin, a biphenolic compound found in turmeric, we show that the kinetic profile is atypical in that it shows a prenucleation period during which there is no observable decrease in NMR peak intensities. PMID:24900390

  8. Genome-wide promoter binding profiling of protein phosphatase-1 and its major nuclear targeting subunits

    PubMed Central

    Verheyen, Toon; Görnemann, Janina; Verbinnen, Iris; Boens, Shannah; Beullens, Monique; Van Eynde, Aleyde; Bollen, Mathieu

    2015-01-01

    Protein phosphatase-1 (PP1) is a key regulator of transcription and is targeted to promoter regions via associated proteins. However, the chromatin binding sites of PP1 have never been studied in a systematic and genome-wide manner. Methylation-based DamID profiling in HeLa cells has enabled us to map hundreds of promoter binding sites of PP1 and three of its major nuclear interactors, i.e. RepoMan, NIPP1 and PNUTS. Our data reveal that the α, β and γ isoforms of PP1 largely bind to distinct subsets of promoters and can also be differentiated by their promoter binding pattern. PP1β emerged as the major promoter-associated isoform and shows an overlapping binding profile with PNUTS at dozens of active promoters. Surprisingly, most promoter binding sites of PP1 are not shared with RepoMan, NIPP1 or PNUTS, hinting at the existence of additional, largely unidentified chromatin-targeting subunits. We also found that PP1 is not required for the global chromatin targeting of RepoMan, NIPP1 and PNUTS, but alters the promoter binding specificity of NIPP1. Our data disclose an unexpected specificity and complexity in the promoter binding of PP1 isoforms and their chromatin-targeting subunits. PMID:25990731

  9. Tissue-Specific Metabolic Profile Study of Moringa oleifera L. Using Nuclear Magnetic Resonance Spectroscopy

    PubMed Central

    Mahmud, Iqbal; Chowdhury, Kamal

    2014-01-01

    Moringa oleifera, an important multipurpose crop, is rich in various phytochemicals: flavonoids, antioxidants, vitamins, minerals and carotenes. The purpose of this study was to profile the groups of metabolites in leaf and stem tissues of M. oleifera. Various sugars, amino acids, and organic acid derivatives were found in all of the M. oleifera tissues with different profiles/peak intensities depending on the tissue. 1D proton nuclear magnetic resonance (NMR) was applied for collecting metabolite spectra. Approximately 30 metabolites with 2 unknown peaks were identified with Chenomx and verified with MMCD databases using carbon data. Among these metabolites, 22 metabolites were identified as common in both leaf and stem tissues. Of the remaining 8 metabolites, 4-aminobutyrate, adenosine, guanosine, tyrosine, and p-cresol were found only in leaf tissues; however, glutamate, glutamine, and tryptophan were found only in stem tissues. Biochemical pathway analysis revealed that 28 identified metabolites were interconnected with 36 different pathways as well as related to different fatty acids and secondary metabolites synthesis biochemical pathways. It is well known that different tissues of M. oleifera have nutritional, medicinal, and therapeutic values; therefore, our main objective is to provide a publicly available M. oliefera tissue specific metabolite database. PMID:26366209

  10. Compact Detection System for High Sensitivity Hydrogen Profiling of Materials by Nuclear Reaction Analysis

    SciTech Connect

    Marble, Daniel Keith; Urban, Ben; Pacheco, Jose

    2009-03-10

    Hydrogen is a ubiquitous contaminant that is known to have dramatic effects on the electrical, chemical, and mechanical properties of many types of materials in even minute quantities. Thus, the detection of hydrogen in materials is of major importance. Nuclear Reaction Analysis (NRA) is a powerful technique for nondestructive profiling hydrogen in materials. However, NRA has found only limited use in many applications because of poor sensitivity due to cosmic ray background (CSRB). Most attempts to eliminate CSRB to achieve ppm detection levels using higher energy nuclear reactions or tons of passive shielding are not compatible with commercial ion beam analysis space and equipment requirements Zimmerman, et al. have previously reported upon a coincidence detector that meets IBA space requirements and reduces the cosmic ray background, but the detector suffers from lower detection efficiency and small sample size. We have replaced the BGO well detector in the Zimmerman coincidence detection scheme with a larger Nal well detector and used faster timing electronics to produce a detector that can handle larger samples with higher detection efficiency, and still eliminate cosmic ray background.

  11. Unexpected Novel Relational Links Uncovered by Extensive Developmental Profiling of Nuclear Receptor Expression

    PubMed Central

    Tavares, Raquel; Sachs, Laurent; Chaumot, Arnaud; Bardet, Pierre-Luc; Escrivà, Héctor; Duffraisse, Maryline; Marchand, Oriane; Safi, Rachid; Thisse, Christine; Laudet, Vincent

    2007-01-01

    Nuclear receptors (NRs) are transcription factors that are implicated in several biological processes such as embryonic development, homeostasis, and metabolic diseases. To study the role of NRs in development, it is critically important to know when and where individual genes are expressed. Although systematic expression studies using reverse transcriptase PCR and/or DNA microarrays have been performed in classical model systems such as Drosophila and mouse, no systematic atlas describing NR involvement during embryonic development on a global scale has been assembled. Adopting a systems biology approach, we conducted a systematic analysis of the dynamic spatiotemporal expression of all NR genes as well as their main transcriptional coregulators during zebrafish development (101 genes) using whole-mount in situ hybridization. This extensive dataset establishes overlapping expression patterns among NRs and coregulators, indicating hierarchical transcriptional networks. This complete developmental profiling provides an unprecedented examination of expression of NRs during embryogenesis, uncovering their potential function during central nervous system and retina formation. Moreover, our study reveals that tissue specificity of hormone action is conferred more by the receptors than by their coregulators. Finally, further evolutionary analyses of this global resource led us to propose that neofunctionalization of duplicated genes occurs at the levels of both protein sequence and RNA expression patterns. Altogether, this expression database of NRs provides novel routes for leading investigation into the biological function of each individual NR as well as for the study of their combinatorial regulatory circuitry within the superfamily. PMID:17997606

  12. Profiling planktonic biomass using element-specific, multicomponent nuclear magnetic resonance spectroscopy.

    PubMed

    Komatsu, Takanori; Kobayashi, Toshiya; Hatanaka, Minoru; Kikuchi, Jun

    2015-06-01

    Planktonic metabolism plays crucial roles in Earth's elemental cycles. Chemical speciation as well as elemental stoichiometry is important for advancing our understanding of planktonic roles in biogeochemical cycles. In this study, a multicomponent solid-state nuclear magnetic resonance (NMR) approach is proposed for chemical speciation of cellular components, using several advanced NMR techniques. Measurements by ssNMR were performed on (13)C and (15)N-labeled Euglena gracilis, a flagellated protist. 3D dipolar-assisted rotational resonance, double-cross-polarization (1)H-(13)C correlation spectroscopy, and (1)H-(13)C solid-state heteronuclear single quantum correlation spectroscopy successively allowed characterization of cellular components. These techniques were then applied to E. gracilis cultured in high and low ammonium media to demonstrate the power of this method for profiling and comparing cellular components. Cellular NMR spectra indicated that ammonium induced both paramylon degradation and amination. Arginine was stored as a nitrogen reserve and ammonium replaced by arginine catabolism via the arginine dihydrolase pathway. (15)N and (31)P cellular ssNMR indicated arginine and polyphosphate accumulation in E. gracilis, respectively. This chemical speciation technique will contribute to environmental research by providing detailed information on environmental chemical properties. PMID:25973714

  13. Nuclear microprobe analysis of solar proton implantation profiles in lunar rock surfaces

    NASA Technical Reports Server (NTRS)

    Stauber, M. C.; Padawer, G. M.; D'Agostino, M. D.; Kamykowski, E.; Brandt, W.; Young, D. A.

    1973-01-01

    Discussion of the results of hydrogen (proton) depth profile concentration analyses conducted on selected Apollo 16 rocks. A modeling of solar particle implantation profiles in lunar rocks is shown to trace the evolvement of these profiles under the combined influence of diffusion of atomic particles implanted in the rock, and rock surface erosion. It is also demonstrated that such diffusion may have a significant effect on the shape of the implantation profiles in certain rock materials.

  14. Protein profiles of bovine placenta derived from somatic cell nuclear transfer.

    PubMed

    Kim, Hong Rye; Kang, Jae Ku; Yoon, Jong Taek; Seong, Hwan Hoo; Jung, Jin Kwan; Lee, Hong Mie; Sik Park, Chang; Jin, Dong Il

    2005-11-01

    Practical application of animal cloning by somatic cell nuclear transfer (SCNT) has been hampered by an extremely low success rate. To address whether placental dysfunction in SCNT causes fetal loss during pregnancy, we have used a global proteomics approach using 2-DE and MS to analyze the differential protein patterns of three placentae from the afterbirth of cases of postnatal death, derived from SCNT of Korean Native cattle, and three normal placentae obtained from the afterbirth of fetuses derived from artificial insemination. Proteins within a pI range of 4.0-7.0 and 6.0-9.0 were analyzed separately by 2-DE in triplicate. A total of approximately 2000 spots were detected in placental 2-DE gels stained with CBB. In the comparison of normal and SCNT samples, 60 spots were identified as differentially expressed proteins, of which 33 spots were up-regulated proteins in SCNT placentae, while 27 spots were down-regulated proteins. Most of the proteins identified in this analysis appeared to be related with protein repair or protection, cytoskeleton, signal transduction, immune system, metabolism, extracellular matrix and remodeling, transcription regulation, cell structure or differentiation and ion transport. One of up-regulated proteins in SCNT was TIMP-2 protein known to be related to extracellular matrix and remodeling during pregnancy. Western blot analysis showed an increased level of TIMP-2 in SCNT placenta compared to normal. Our results revealed composite profiles of key proteins involved in abnormal placenta derived from SCNT, and suggested expression abnormality of these genes in SCNT placenta, resulting in fetal losses following SCNT. PMID:16196098

  15. C and N depth profiles of SiCN layers determined with nuclear reaction analyses and AES

    NASA Astrophysics Data System (ADS)

    Link, F.; Baumann, H.; Bethge, K.; Klewe-Nebenius, H.; Bruns, M.

    1998-04-01

    Si 1C xN y layers were prepared by sequential implantation of 40 keV 13C- and 50 keV 15N-ions into c-Si <1 1 1> samples near RT. The carbon and nitrogen depth distributions were measured using the resonant nuclear (p,γ) reactions 15N(p,αγ) 12C at Eres=429 keV and 13C(p,γ) 14N at Eres=1748 keV, respectively. The measured raw data of depth profiling (gamma yield versus the proton beam energy) are converted to concentration-depth profiles of the elements C, N and Si with a common depth scale by using a new developed computer algorithm. These concentration profiles are compared with those obtained with Auger Electron Spectroscopy (AES) and non-Rutherford Backscattering Spectrometry (n-RBS).

  16. An intrinsically safe facility for forefront research and training on nuclear technologies — Target profile optimization

    NASA Astrophysics Data System (ADS)

    Ciotti, Marco

    2014-04-01

    A simple 1D algorithm for a spallation target profile optimization is presented. The profile efficacy is tested considering different real particle beams profiles impinging on the target. The results are commented in terms of power non-homogeneity for unit length for different beam/profile choices, showing a non-trivial parameters selection. In details, target shaping optimization is possible knowing the beam parameters with a large benefit for surface power homogeneity, but results of the simulations show that a small increase of the beam dimension, or a beam shift with respect to the assumed one, could rapidly erase the advantages.

  17. Partners: Promoting Accessible Recreation.

    ERIC Educational Resources Information Center

    Sable, Janet; Gravink, Jill

    1995-01-01

    The Promoting Accessible Recreation through Networking, Education, Resources and Services (PARTNERS) Project, a partnership between Northeast Passage, the University of New Hampshire, and Granite State Independent Living Foundation, helps create barrier-free recreation for individuals with physical disabilities. The paper describes PARTNERS and…

  18. Mirincamycin, an old candidate for malaria combination treatment and prophylaxis in the 21st century: in vitro interaction profiles with potential partner drugs in continuous culture and field isolates

    PubMed Central

    2014-01-01

    Background Spreading resistance of Plasmodium falciparum to existing drugs calls for the search for novel anti-malarial drugs and combinations for the treatment of falciparum malaria. Methods In vitro and ex vivo investigations were conducted with fresh P. falciparum field isolates and culture-adapted P. falciparum clones to evaluate the anti-malarial potential of mirincamycin, a lincosamide, alone and in combination with tafenoquine (TQ), dihydroartemisinin (DHA), and chloroquine (CQ). All samples were tested in a histidine-rich protein 2 (HRP2) drug susceptibility assay. Results Interaction analysis showed additive to synergistic interaction profiles with these potential partner drugs, with an overall geometric mean fractional inhibitory concentration at 50% inhibition (FIC50) of 0.78, 0.80 and 0.80 for mirincamycin with TQ, DHA, and CQ, respectively. Antagonism was not found in any of the tested field isolates or clones. The strongest tendency toward synergy (i.e. the lowest FIC) was seen with a combination ratio of 1:0.27 to 1:7.2 (mean 1:2.7) for the combination with tafenoquine. The optimal combination ratios for DHA and CQ were 1:444.4 to 1:36,000 (mean 1:10,755.5) and 1:2.7 to 1:216 (mean 1:64.5), respectively. No evidence of an activity correlation (i.e. potential cross-resistance) with DHA, mefloquine, quinine or chloroquine was seen whereas a significant correlation with the activity of clindamycin and azithromycin was detected. Conclusions Mirincamycin combinations may be promising candidates for further clinical investigations in the therapy and prophylaxis of multidrug-resistant falciparum malaria or in combination with 4 or 8-aminoquinolines for the treatment and relapse prevention of vivax malaria. PMID:24916383

  19. (1)H-Nuclear Magnetic Resonance-Based Plasma Metabolic Profiling of Dairy Cows with Fatty Liver.

    PubMed

    Xu, Chuang; Sun, Ling-Wei; Xia, Cheng; Zhang, Hong-You; Zheng, Jia-San; Wang, Jun-Song

    2016-02-01

    Fatty liver is a common metabolic disorder of dairy cows during the transition period. Historically, the diagnosis of fatty liver has involved liver biopsy, biochemical or histological examination of liver specimens, and ultrasonographic imaging of the liver. However, more convenient and noninvasive methods would be beneficial for the diagnosis of fatty liver in dairy cows. The plasma metabolic profiles of dairy cows with fatty liver and normal (control) cows were investigated to identify new biomarkers using (1)H nuclear magnetic resonance. Compared with the control group, the primary differences in the fatty liver group included increases in β-hydroxybutyric acid, acetone, glycine, valine, trimethylamine-N-oxide, citrulline, and isobutyrate, and decreases in alanine, asparagine, glucose, γ-aminobutyric acid glycerol, and creatinine. This analysis revealed a global profile of endogenous metabolites, which may present potential biomarkers for the diagnosis of fatty liver in dairy cows. PMID:26732447

  20. 1H-Nuclear Magnetic Resonance-Based Plasma Metabolic Profiling of Dairy Cows with Fatty Liver

    PubMed Central

    Xu, Chuang; Sun, Ling-wei; Xia, Cheng; Zhang, Hong-you; Zheng, Jia-san; Wang, Jun-song

    2016-01-01

    Fatty liver is a common metabolic disorder of dairy cows during the transition period. Historically, the diagnosis of fatty liver has involved liver biopsy, biochemical or histological examination of liver specimens, and ultrasonographic imaging of the liver. However, more convenient and noninvasive methods would be beneficial for the diagnosis of fatty liver in dairy cows. The plasma metabolic profiles of dairy cows with fatty liver and normal (control) cows were investigated to identify new biomarkers using 1H nuclear magnetic resonance. Compared with the control group, the primary differences in the fatty liver group included increases in β-hydroxybutyric acid, acetone, glycine, valine, trimethylamine-N-oxide, citrulline, and isobutyrate, and decreases in alanine, asparagine, glucose, γ-aminobutyric acid glycerol, and creatinine. This analysis revealed a global profile of endogenous metabolites, which may present potential biomarkers for the diagnosis of fatty liver in dairy cows. PMID:26732447

  1. Profiles of embryonic nuclear protein binding to the proximal promoter region of the soybean β-conglycinin α subunit gene.

    PubMed

    Yoshino, M; Tsutsumi, K; Kanazawa, A

    2015-01-01

    β-Conglycinin, a major component of seed storage protein in soybean, comprises three subunits: α, α' and β. The expression of genes for these subunits is strictly controlled during embryogenesis. The proximal promoter region up to 245 bp upstream of the transcription start site of the α subunit gene sufficiently confers spatial and temporal control of transcription in embryos. Here, the binding profile of nuclear proteins in the proximal promoter region of the α subunit gene was analysed. DNase I footprinting analysis indicated binding of proteins to the RY element and DNA regions including box I, a region conserved in cognate gene promoters. An electrophoretic mobility shift assay (EMSA) using different portions of box I as a probe revealed that multiple portions of box I bind to nuclear proteins. In addition, an EMSA using nuclear proteins extracted from embryos at different developmental stages indicated that the levels of major DNA-protein complexes on box I increased during embryo maturation. These results are consistent with the notion that box I is important for the transcriptional control of seed storage protein genes. Furthermore, the present data suggest that nuclear proteins bind to novel motifs in box I including 5'-TCAATT-3' rather than to predicted cis-regulatory elements. PMID:24943483

  2. Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors.

    PubMed

    Padilla, Philip Ian; Pacheco-Rodriguez, Gustavo; Moss, Joel; Vaughan, Martha

    2004-03-01

    Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (BIG1) is an approximately 200-kDa brefeldin A-inhibited guanine nucleotide-exchange protein that preferentially activates ADP-ribosylation factor 1 (ARF1) and ARF3. BIG1 was found in cytosol in a multiprotein complex with a similar ARF-activating protein, BIG2, which is also an A kinase-anchoring protein. In HepG2 cells growing with serum, BIG1 was primarily cytosolic and Golgi-associated. After incubation overnight without serum, a large fraction of endogenous BIG1 was in the nuclei. By confocal immunofluorescence microscopy, BIG1 was localized with nucleoporin p62 at the nuclear envelope (probably during nucleocytoplasmic transport) and also in nucleoli, clearly visible against the less concentrated overall matrix staining. BIG1 was also identified by Western blot analyses in purified subnuclear fractions (e.g., nucleoli and nuclear matrix). Antibodies against BIG1, nucleoporin, or nucleolin coimmunoprecipitated the other two proteins from purified nuclei. In contrast, BIG2 was not associated with nuclear BIG1. Also of note, ARF was never detected among proteins precipitated from purified nuclei by anti-BIG1 antibodies, although microscopically the two proteins do appear sometimes to be colocalized in the nucleus. These data are consistent with independent intracellular movements and actions of BIG1 and BIG2, and they are also evidence of the participation of BIG1 in both Golgi and nuclear functions. PMID:14973189

  3. {beta}-Catenin can act as a nuclear import receptor for its partner transcription factor, lymphocyte enhancer factor-1 (lef-1)

    SciTech Connect

    Asally, Munehiro; Yoneda, Yoshihiro . E-mail: yyoneda@anat3.med.osaka-u.ac.jp

    2005-08-15

    Nuclear accumulation of {beta}-catenin plays an important role in the Wnt signaling pathway. In the nucleus, {beta}-catenin acts as a transcriptional co-activator for TCF/LEF family of transcription factors. It has been shown that lef-1 contains a typical basic type nuclear localization signal (NLS) and is transported into the nucleus by the conventional import pathway. In this study, we found that a mutant lef-1 lacking the classical NLS accumulated in the nucleus of living cells, when {beta}-catenin was co-expressed. In addition, in a cell-free import assay, lef-1 migrated into the nucleus in the presence of {beta}-catenin alone without any other soluble factors. In contrast, another mutant lef-1 lacking the {beta}-catenin binding domain failed to migrate into the nucleus, even in the presence of {beta}-catenin. These findings indicate that {beta}-catenin alone can mediate the nuclear import of lef-1 through the direct binding. Collectively, we propose that there are two distinct pathways for the nuclear import of lef-1: importin {alpha}/{beta}-mediated and {beta}-catenin-mediated one, which provides a novel paradigm for Wnt signaling pathway.

  4. In Situ Effective Diffusion Coefficient Profiles in Live Biofilms Using Pulsed-Field Gradient Nuclear Magnetic Resonance

    SciTech Connect

    Renslow, Ryan S.; Majors, Paul D.; McLean, Jeffrey S.; Fredrickson, Jim K.; Ahmed, B.; Beyenal, Haluk

    2010-08-15

    Diffusive mass transfer in biofilms is characterized by the effective diffusion coefficient. It is well-documented that the effective diffusion coefficient can vary by location in a biofilm. The current literature is dominated by effective diffusion coefficient measurements for distinct cell clusters and stratified biofilms showing this spatial variation. Regardless of whether distinct cell clusters or surface-averaging methods are used, position-dependent measurements of the effective diffusion coefficient are currently: 1) invasive to the biofilm, 2) performed under unnatural conditions, 3) lethal to cells, and/or 4) spatially restricted to only certain regions of the biofilm. Invasive measurements can lead to inaccurate results and prohibit further (time dependent) measurements which are important for the mathematical modeling of biofilms. In this study our goals were to: 1) measure the effective diffusion coefficient for water in live biofilms, 2) monitor how the effective diffusion coefficient changes over time under growth conditions, and 3) correlate the effective diffusion coefficient with depth in the biofilm. We measured in situ two-dimensional effective diffusion coefficient maps within Shewanella oneidensis MR-1biofilms using pulsed-field gradient nuclear magnetic resonance methods, and used them to calculate surface-averaged relative effective diffusion coefficient (Drs) profiles. We found that 1) Drs decreased from the top of the biofilm to the bottom, 2) Drs profiles differed for biofilms of different ages, 3) Drs profiles changed over time and generally decreased with time, 4) all the biofilms showed very similar Drs profiles near the top of the biofilm, and 5) the Drs profile near the bottom of the biofilm was different for each biofilm. Practically, our results demonstrate that advanced biofilm models should use a variable effective diffusivity which changes with time and location in the biofilm.

  5. In situ effective diffusion coefficient profiles in live biofilms using pulsed-field gradient nuclear magnetic resonance

    PubMed Central

    Renslow, Ryan S.; Majors, Paul D.; McLean, Jeffrey S.; Fredrickson, Jim K.; Ahmed, Bulbul; Beyenal, Haluk

    2010-01-01

    Diffusive mass transfer in biofilms is characterized by the effective diffusion coefficient. It is well-documented that the effective diffusion coefficient can vary by location in a biofilm. The current literature is dominated by effective diffusion coefficient measurements for distinct cell clusters and stratified biofilms showing this spatial variation. Regardless of whether distinct cell clusters or surface-averaging methods are used, position-dependent measurements of the effective diffusion coefficient are currently: 1) invasive to the biofilm, 2) performed under unnatural conditions, 3) lethal to cells, and/or 4) spatially restricted to only certain regions of the biofilm. Invasive measurements can lead to inaccurate results and prohibit further (time-dependent) measurements which are important for the mathematical modeling of biofilms. In this study our goals were to: 1) measure the effective diffusion coefficient for water in live biofilms, 2) monitor how the effective diffusion coefficient changes over time under growth conditions, and 3) correlate the effective diffusion coefficient with depth in the biofilm. We measured in situ two-dimensional effective diffusion coefficient maps within Shewanella oneidensis MR-1 biofilms using pulsed-field gradient nuclear magnetic resonance methods, and used them to calculate surface-averaged relative effective diffusion coefficient (Drs) profiles. We found that 1) Drs decreased from the top of the biofilm to the bottom, 2) Drs profiles differed for biofilms of different ages, 3) Drs profiles changed over time and generally decreased with time, 4) all the biofilms showed very similar Drs profiles near the top of the biofilm, and 5) the Drs profile near the bottom of the biofilm was different for each biofilm. Practically, our results demonstrate that advanced biofilm models should use a variable effective diffusivity which changes with time and location in the biofilm. PMID:20589671

  6. Nuclear Magnetic Resonance (NMR) Spectroscopy For Metabolic Profiling of Medicinal Plants and Their Products.

    PubMed

    Kumar, Dinesh

    2016-09-01

    NMR spectroscopy has multidisciplinary applications, including excellent impact in metabolomics. The analytical capacity of NMR spectroscopy provides information for easy qualitative and quantitative assessment of both endogenous and exogenous metabolites present in biological samples. The complexity of a particular metabolite and its contribution in a biological system are critically important for understanding the functional state that governs the organism's phenotypes. This review covers historical aspects of developments in the NMR field, its applications in chemical profiling, metabolomics, and quality control of plants and their derived medicines, foods, and other products. The bottlenecks of NMR in metabolic profiling are also discussed, keeping in view the future scope and further technological interventions. PMID:26575437

  7. Absolute hydrogen depth profiling using the resonant 1H(15N, αγ)12C nuclear reaction

    NASA Astrophysics Data System (ADS)

    Reinhardt, Tobias P.; Akhmadaliev, Shavkat; Bemmerer, Daniel; Stöckel, Klaus; Wagner, Louis

    2016-08-01

    Resonant nuclear reactions are a powerful tool for the determination of the amount and profile of hydrogen in thin layers of material. Usually, this tool requires the use of a standard of well-known composition. The present work, by contrast, deals with standard-less hydrogen depth profiling. This approach requires precise nuclear data, e.g. on the widely used 1 H(15 N, αγ)12 C reaction, resonant at 6.4 MeV 15 N beam energy. Here, the strongly anisotropic angular distribution of the emitted γ -rays from this resonance has been re-measured, resolving a previous discrepancy. Coefficients of (0.38 ± 0.04) and (0.80 ± 0.04) have been deduced for the second and fourth order Legendre polynomials, respectively. In addition, the resonance strength has been re-evaluated to (25.0 ± 1.5) eV, 10% higher than previously reported. A simple working formula for the hydrogen concentration is given for cases with known γ -ray detection efficiency. Finally, the absolute approach is illustrated using two examples.

  8. Partnering for the Future

    NASA Video Gallery

    With an ambitious new direction and a new way of doing business, NASA's Kennedy Space Center is embracing a new relationship with the commercial space industry and other partners to build a multi-u...

  9. Resources for Partners

    SciTech Connect

    2006-02-01

    This DOE Industrial Technologies Program fact sheet describes the resources and opportunities available to partners how manufacturing plants can save energy and money by making energy efficiency improvements to their industrial process heating systems.

  10. Specificity of antinuclear autoantibodies recognizing the dense fine speckled nuclear pattern: Preferential targeting of DFS70/LEDGFp75 over its interacting partner MeCP2.

    PubMed

    Basu, Anamika; Woods-Burnham, Leanne; Ortiz, Greisha; Rios-Colon, Leslimar; Figueroa, Johnny; Albesa, Roger; Andrade, Luis E; Mahler, Michael; Casiano, Carlos A

    2015-12-01

    Human antinuclear autoantibodies (ANAs) targeting the dense fine speckled (DFS) nuclear protein DFS70, commonly known as lens epithelium derived growth factor p75 (LEDGFp75), present a clinical puzzle since their significance remains elusive. While their frequencies are low in ANA-positive autoimmune rheumatic diseases, they are relatively elevated in clinical laboratory referrals, diverse inflammatory conditions, and 'apparently' healthy individuals. We reported previously that DFS70/LEDGFp75 is an autoantigen in prostate cancer that closely interacts with another 70kD DFS nuclear protein, methyl CpG binding protein 2 (MeCP2). This led us to investigate if anti-DFS sera exclusively target DFS70/LEDGFp75 or also recognize MeCP2. Using several complementary autoantibody detection platforms and cellular/molecular approaches we evaluated 65 human sera producing anti-DFS autoantibodies. Our results show that these antibodies are highly specific for DFS70/LEDGFp75 and do not target MeCP2. Establishing the specificity of anti-DFS autoantibodies has implications for increasing our understanding of their biological significance and clinical utility. PMID:26235378

  11. Protein profiles in cortical and nuclear regions of aged human donor lenses: A confocal Raman microspectroscopic and imaging study.

    PubMed

    Vrensen, Gijs F J M; Otto, Cees; Lenferink, Aufried; Liszka, Barbara; Montenegro, Gustavo A; Barraquer, Rafael I; Michael, Ralph

    2016-04-01

    A combination of Raman spectroscopy, imaging, hierarchical cluster analysis (HCA) and peak ratio analysis was used to analyze protein profiles in the superficial cortex (SC), deep cortex (DC) and nucleus of old human lenses with cortical, nuclear and mixed cataracts. No consistent differences were observed in protein spectra and after cluster analysis between the three locations irrespective of the presence or absence of cortical opacities and/or coloration. A sharp increase (∼15%-∼33%) in protein content from SC to DC, normal for human lenses, was found in 7 lenses. In 4 lenses, characterized by the absence of cortical opacities, the SC has a protein content of ∼35%. A significant increase in the disulfide-to-protein ratio is found only in the SC of the 7 cortical cataracts. No changes were found in sulfhydryl-to-protein ratio. The relative contents of α-helices and β-sheets increase from SC to nucleus. β-Sheets are more common in the SC of lenses with cortical cataract. The absence of significant and consistent changes in protein profiles between nucleus and cortex even in cases of severe coloration is not favoring the prevailing concept that ubiquitous protein oxidation is a key factor for age related nuclear (ARN) cataracts. The observations favor the idea that multilamellar bodies or protein aggregates at very low volume densities are responsible for the rise in Mie light scatter as a main cause of ARN cataracts leaving the short-range-order of the fiber cytoplasm largely intact. The absence of significant changes in the protein spectra of the deep cortical opacities, milky white as a result of the presence of vesicle-like features, indicate they are packed with relatively undisturbed crystallins. PMID:26611157

  12. Nuclear factor 45 of half smooth tongue sole Cynoglossus semilaevis: gene structure, expression profile, and immunoregulatory property.

    PubMed

    Chi, Heng; Xiao, Zhi-zhong; Sun, Li

    2013-09-01

    Nuclear factor 45 (NF45) is a component of the protein complex called nuclear factor of activated T-cells (NFAT), which in mammals regulates interleukin (IL)-2 expression. To date very little is known about fish NF45. In this study, we identified a NF45 (named CsNF45) from half smooth tongue sole Cynoglossus semilaevis and examined its gene organization, expression profile, and regulatory function. We found that CsNF45 is composed of 387 residues and shares 90.3%-97.9% overall sequence identities with the NF45 of human and teleosts. Genetic analysis showed that the genomic sequence of the ORF region of CsNF45 consists of 14 exons and 13 introns. Constitutive expression of CsNF45 occurred in multiple tissues including gill, muscle, brain, heart, liver, head kidney, spleen, and gut. Experimental infection with viral and bacterial pathogens upregulated the expression of CsNF45 in head kidney and spleen in a time-dependent manner. Transient transfection analysis showed that CsNF45 was localized in the nucleus and able to stimulate the activity of mouse IL-2 promoter. These results indicate that CsNF45 possesses immunoregulatory property and is possibly involved in host immune defense against bacterial and viral infection. PMID:23872474

  13. Collaboration with Community Partners

    ERIC Educational Resources Information Center

    Sterling, Donna R.; Frazier, Wendy M.

    2006-01-01

    For eight years, relationships with community partners have been the mainstay of a science enrichment program for secondary students. Through the use of problem-based learning, science classes use, the techniques and tools of scientists to solve authentic problems directly related to students' interests and needs. In this article, the author…

  14. Intimate partner violence.

    PubMed

    Pibernat, Artur Dalfó

    2016-05-01

    IT IS with great interest that I read the article 'Identifying signs of intimate partner violence' (Art & science) by Ali et al in February's issue of Emergency Nurse. The issue has been widely recognised as one which needs promoting among adolescents in schools and in nurse education. PMID:27165383

  15. Partnering with Homeschoolers.

    ERIC Educational Resources Information Center

    Caruana, Vicki

    1999-01-01

    Homeschooled students from families representing all ethnicities, socioeconomic backgrounds, and education levels are increasingly entering public schools part-time. This article explains how school administrators, teachers, parents, and homeschoolers can become partners. Tips are provided on classroom deportment, parental aspirations, and…

  16. Partners of the Community.

    ERIC Educational Resources Information Center

    Reese, Susan

    2002-01-01

    Oklahoma has a long tradition of partnering with the community and its career-tech system is viewed as the economic development arm of the Oklahoma Public School system. A partnership between the Tri County Technology Center and University of Oklahoma, for example, involves dental hygiene students in providing oral health care for poor rural…

  17. The Partners Program.

    ERIC Educational Resources Information Center

    Raughton, Jim L.

    In response to alarming drop-out rates among high school students and indications that the emerging workforce will be predominantly minority and disadvantaged, the Colorado Community College and Occupational Education System (CCCOES) developed the Partners program to encourage minority students from as early as seventh grade to remain and succeed…

  18. Predictability of Conversation Partners

    NASA Astrophysics Data System (ADS)

    Takaguchi, Taro; Nakamura, Mitsuhiro; Sato, Nobuo; Yano, Kazuo; Masuda, Naoki

    2011-08-01

    Recent developments in sensing technologies have enabled us to examine the nature of human social behavior in greater detail. By applying an information-theoretic method to the spatiotemporal data of cell-phone locations, [C. Song , ScienceSCIEAS0036-8075 327, 1018 (2010)] found that human mobility patterns are remarkably predictable. Inspired by their work, we address a similar predictability question in a different kind of human social activity: conversation events. The predictability in the sequence of one’s conversation partners is defined as the degree to which one’s next conversation partner can be predicted given the current partner. We quantify this predictability by using the mutual information. We examine the predictability of conversation events for each individual using the longitudinal data of face-to-face interactions collected from two company offices in Japan. Each subject wears a name tag equipped with an infrared sensor node, and conversation events are marked when signals are exchanged between sensor nodes in close proximity. We find that the conversation events are predictable to a certain extent; knowing the current partner decreases the uncertainty about the next partner by 28.4% on average. Much of the predictability is explained by long-tailed distributions of interevent intervals. However, a predictability also exists in the data, apart from the contribution of their long-tailed nature. In addition, an individual’s predictability is correlated with the position of the individual in the static social network derived from the data. Individuals confined in a community—in the sense of an abundance of surrounding triangles—tend to have low predictability, and those bridging different communities tend to have high predictability.

  19. Partner Facilitation and Partner Interference in Individuals' Weight Loss Goals.

    PubMed

    Theiss, Jennifer A; Carpenter, Amanda M; Leustek, John

    2016-08-01

    Drawing on the logic of the relational turbulence model, this study examined the ways in which romantic partners facilitate and interfere with individuals' weight loss goals. Participants (N = 122) described the ways in which their romantic partner had recently helped or hindered their weight loss at four times over the course of 2 months. We conducted a content analysis of responses to identify themes of partner facilitation (Research Question 1 [RQ1]) and partner interference (RQ2) in individuals' weight loss goals. Results revealed seven themes of partner facilitation: (a) partner enabling diet, (b) motivation and encouragement, (c) emotional support and positive reinforcement, (d) exercising together, (e) partner enabling exercise, (f) dieting together, and (g) relationship influence and priorities. Four themes of partner interference emerged in the data: (a) inability to plan for healthy meals, (b) inability to control the food environment, (c) preventing or discouraging exercise, and (d) emotional or relational discouragement. PMID:25904678

  20. Nuclear Microprobe using Elastic Recoil Detection (ERD) for Hydrogen Profiling in High Temperature Protonic Conductors

    NASA Technical Reports Server (NTRS)

    Berger, Pascal; Sayir, Ali; Berger, Marie-Helene

    2004-01-01

    The interaction between hydrogen and various high temperature protonic conductors (HTPC) has not been clearly understood due to poor densification and unreacted secondary phases. the melt-processing technique is used in producing fully dense simple SrCe(0.9)Y (0.10) O(3-delta) and complex Sr3Ca(1+x)Nb(2+x)O(9-delta) perovskites that can not be achieved by solid-state sintering. the possibilities of ion beam analysis have been investigated to quantify hydrogen distribution in HTPC perovskites subjected to water heat treatment. Nuclear microprobe technique is based on the interactions of a focused ion beam of MeV light ions (H-1, H-2, He-3, He-4,.) with the sample to be analyzed to determine local elemental concentrations at the cubic micrometer scale, the elastic recoil detection analysis technique (ERDA) has been carried out using He-4(+) microbeams and detecting the resulting recoil protons. Mappings of longitudinal sections of water treated SrCeO3 and Sr(Ca(1/3)Nb(2/3))O3 perovskites have been achieved, the water treatment strongly alters the surface of simple SrCe(0.9)Y(0.10)O(3-delta) perovskite. From Rutherford Back Scattering measurements (RBS), both Ce depletion and surface re-deposition is evidenced. the ERDA investigations on water treated Sr3Ca(1+x)Nb(2+x)O(9-delta) perovskite did not exhibit any spatial difference for the hydrogen incorporation from the surface to the centre. the amount of hydrogen incorporation for Sr3Ca(1+x)Nb(2+x)O(9-delta) was low and required further development of two less conventional techniques, ERDA in forward geometry and forward elastic diffusion H-1(p,p) H-1 with coincidence detection.

  1. Parents as Writing Partners

    ERIC Educational Resources Information Center

    Ehrenworth, Mary

    2014-01-01

    Parents know that writing is essential to their children's success, and they're eager to help their children become good writers. But often, they're at a loss about how to help. Instead of leaving them in the dark, schools can make parents into valuable writing partners by giving them a toolkit of guidelines for coaching writers.…

  2. Metabolic profiling of a Schistosoma mansoni infection in mouse tissues using magic angle spinning-nuclear magnetic resonance spectroscopy.

    PubMed

    Li, Jia V; Holmes, Elaine; Saric, Jasmina; Keiser, Jennifer; Dirnhofer, Stephan; Utzinger, Jürg; Wang, Yulan

    2009-04-01

    In order to enhance our understanding of physiological and pathological consequences of a patent Schistosoma mansoni infection in the mouse, we examined the metabolic responses of different tissue samples recovered from the host animal using a metabolic profiling strategy. Ten female NMRI mice were infected with approximately 80 S. mansoni cercariae each, and 10 uninfected age- and sex-matched animals served as controls. At day 74 post infection (p.i.), mice were killed and jejunum, ileum, colon, liver, spleen and kidney samples were removed. We employed (1)H magic angle spinning-nuclear magnetic resonance spectroscopy to generate tissue-specific metabolic profiles. The spectral data were analyzed using multivariate modelling methods including an orthogonal signal corrected-projection to latent structure analysis and hierarchical principal component analysis to assess the differences and/or similarities in metabolic responses between infected and non-infected control mice. Most tissues obtained from S. mansoni-infected mice were characterized by high levels of amino acids, such as leucine, isoleucine, lysine, glutamine and asparagine. High levels of membrane phospholipid metabolites, including glycerophosphoryl choline and phosphoryl choline were found in the ileum, colon, liver and spleen of infected mice. Additionally, low levels of energy-related metabolites, including lipids, glucose and glycogen were observed in ileum, spleen and liver samples of infected mice. Energy-related metabolites in the jejunum, liver and renal medulla were found to be positively correlated with S. mansoni worm burden upon dissection. These findings show that a patent S. mansoni infection causes clear disruption of metabolism in a range of tissues at a molecular level, which can be interpreted in relation to the previously reported signature in a biofluid (i.e. urine), giving further evidence of the global effect of the infection. PMID:19068218

  3. Identification of 1- and 3-methylhistidine as biomarkers of skeletal muscle toxicity by nuclear magnetic resonance-based metabolic profiling.

    PubMed

    Aranibar, Nelly; Vassallo, Jeffrey D; Rathmacher, John; Stryker, Steve; Zhang, Yingru; Dai, Jun; Janovitz, Evan B; Robertson, Don; Reily, Michael; Lowe-Krentz, Linda; Lehman-McKeeman, Lois

    2011-03-01

    Nuclear magnetic resonance (NMR)-based metabolomic profiling identified urinary 1- and 3-methylhistidine (1- and 3-MH) as potential biomarkers of skeletal muscle toxicity in Sprague-Dawley rats following 7 and 14 daily doses of 0.5 or 1mg/kg cerivastatin. These metabolites were highly correlated to sex-, dose- and time-dependent development of cerivastatin-induced myotoxicity. Subsequently, the distribution and concentration of 1- and 3-MH were quantified in 18 tissues by gas chromatography-mass spectrometry. The methylhistidine isomers were most abundant in skeletal muscle with no fiber or sex differences observed; however, 3-MH was also present in cardiac and smooth muscle. In a second study, rats receiving 14 daily doses of 1mg/kg cerivastatin (a myotoxic dose) had 6- and 2-fold elevations in 1- and 3-MH in urine and had 11- and 3-fold increases in 1- and 3-MH in serum, respectively. Selectivity of these potential biomarkers was tested by dosing rats with the cardiotoxicant isoproterenol (0.5mg/kg), and a 2-fold decrease in urinary 1- and 3-MH was observed and attributed to the anabolic effect on skeletal muscle. These findings indicate that 1- and 3-MH may be useful urine and serum biomarkers of drug-induced skeletal muscle toxicity and hypertrophy in the rat, and further investigation into their use and limitations is warranted. PMID:21094120

  4. Interaction Quality during Partner Reading

    PubMed Central

    Meisinger, Elizabeth B.; Schwanenflugel, Paula J.; Bradley, Barbara A.; Stahl, Steven A.

    2009-01-01

    The influence of social relationships, positive interdependence, and teacher structure on the quality of partner reading interactions was examined. Partner reading, a scripted cooperative learning strategy, is often used in classrooms to promote the development of fluent and automatic reading skills. Forty-three pairs of second grade children were observed during partner reading sessions taking place in 12 classrooms. The degree to which the partners displayed social cooperation (instrumental support, emotional support, and conflict management) and on/off task behavior was evaluated. Children who chose their own partners showed greater social cooperation than those children whose teacher selected their partner. However, when the positive interdependence requirements of the task were not met within the pair (neither child had the skills to provide reading support or no one needed support), lower levels of on-task behavior were observed. Providing basic partner reading script instruction at the beginning of the year was associated with better social cooperation during partner reading, but providing elaborated instruction or no instruction was associated with poorer social cooperation. It is recommended that teachers provide basic script instruction and allow children to choose their own partners. Additionally, pairings of low ability children with other low ability children and high ability children with other high ability children should be avoided. Teachers may want to suggest alternate partners for children who inadvertently choose such pairings or adjust the text difficulty to the pair. Overall, partner reading seems to be an enjoyable pedagogical strategy for teaching reading fluency. PMID:19830259

  5. FEAR OF PAST ABUSIVE PARTNER(S) IMPACTS CURRENT POSTTRAUMATIC STRESS AMONG WOMEN EXPERIENCING PARTNER VIOLENCE

    PubMed Central

    Jaquier, Véronique; Sullivan, Tami P.

    2014-01-01

    This study examines the impact of fear of past abusive partner(s) on posttraumatic stress among 212 community-recruited women currently exposed to intimate partner violence (IPV). The path analysis model tested explained 60% of the variation in IPV-related posttraumatic stress. Findings revealed that fear of past abusive partner(s) was uniquely associated with the severity of current posttraumatic stress symptoms over and above the impact of current IPV or childhood abuse and neglect. Future research should continue examining women's subjective emotional experience of past and current victimization so as to further inform both clinical practice and intervention planning. PMID:24590514

  6. Classifying partner femicide.

    PubMed

    Dixon, Louise; Hamilton-Giachritsis, Catherine; Browne, Kevin

    2008-01-01

    The heterogeneity of domestic violent men has long been established. However, research has failed to examine this phenomenon among men committing the most severe form of domestic violence. This study aims to use a multidimensional approach to empirically construct a classification system of men who are incarcerated for the murder of their female partner based on the Holtzworth-Munroe and Stuart (1994) typology. Ninety men who had been convicted and imprisoned for the murder of their female partner or spouse in England were identified from two prison samples. A content dictionary defining offense and offender characteristics associated with two dimensions of psychopathology and criminality was developed. These variables were extracted from institutional records via content analysis and analyzed for thematic structure using multidimensional scaling procedures. The resultant framework classified 80% (n = 72) of the sample into three subgroups of men characterized by (a) low criminality/low psychopathology (15%), (b) moderate-high criminality/ high psychopathology (36%), and (c) high criminality/low-moderate psychopathology (49%). The latter two groups are akin to Holtzworth-Munroe and Stuart's (1994) generally violent/antisocial and dysphoric/borderline offender, respectively. The implications for intervention, developing consensus in research methodology across the field, and examining typologies of domestic violent men prospectively are discussed. PMID:18087033

  7. Depth distribution of 137Cs, 134Cs, and 131I in soil profile after Fukushima Dai-ichi Nuclear Power Plant Accident.

    PubMed

    Kato, Hiroaki; Onda, Yuichi; Teramage, Mengistu

    2012-09-01

    Soil, vegetation and other ecological compartments are expected to be highly contaminated by the deposited radionuclides after the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident triggered by a magnitude 9.0 earthquake and the resulting tsunami on 11 March 2011. However, there is no field measurement data on the depth distributions of radiocaesium and (131)I concentrations in soil profile. In this study, the depth distribution of the deposited radionuclides in the cultivated soil profile was investigated in one of the most contaminated area after FDNPP accident. The result of this study demonstrated that greater than 86% of total radiocaesium and 79% of total (131)I were absorbed in the upper 2.0 cm in the soil profile. The relaxation mass depth (h(0)) derived from the depth distribution of radiocaesium and (131)I in the soil profile at the study site were 9.1 kg m(-2) and 10.4 kg m(-2), respectively. The h(0) of (137)Cs in the studied soil profile was greater than those for the cultivated soils nearby the Chernobyl NPP. The positive relationship was found between clay content of topsoil and the h(0) of (137)Cs. However, further analysis is required to clarify the effect of clay content on the initial penetration depth of deposited (137)Cs in soil profile. PMID:22029969

  8. Metabolic profiles using (1)H-nuclear magnetic resonance spectroscopy in postpartum dairy cows with ovarian inactivity.

    PubMed

    Xu, Chuchu; Xia, Cheng; Sun, Yuhang; Xiao, Xinhuan; Wang, Gang; Fan, Ziling; Shu, Shi; Zhang, Hongyou; Xu, Chuang; Yang, Wei

    2016-10-01

    To understand the differences in metabolic changes between cows with ovarian inactivity and estrus cows, we selected cows at 60-90 days postpartum from an intensive dairy farm. According to clinical manifestations, B-ultrasound scan, rectal examination, 10 cows were assigned to the estrus group (A) and 10 to the ovarian inactivity group (B). All plasma samples were analyzed by (1)H-nuclear magnetic resonance spectroscopy to compare plasma metabolomic profiles between the groups. We used multivariate pattern recognition to screen for different metabolites in plasma of anestrus cows. Compared with normal estrous cows, there were abnormalities in 12 kinds of metabolites in postpartum cows with ovarian inactivity (|r|> 0.602), including an increase in acetic acid (r = -0.817), citric acid (r = -0.767), and tyrosine (r = -0.714), and a decrease in low-density lipoprotein (r = 0.820), very low-density lipoprotein (r = 0.828), lipids (r = 0.769), alanine (r = 0.816), pyruvate (r = 0.721), creatine (r = 0.801), choline (r = 0.639), phosphorylcholine (r = 0.741), and glycerophosphorylcholine (r = 0.881). These metabolites were closely related to abnormality of glucose, amino acid, lipoprotein and choline metabolism, which may disturb the normal estrus. The decrease in plasma creatine and the increase in tyrosine were new changes for ovarian inactivity of postpartum cows. The decrease in plasma creatine and choline and the increase in tyrosine and p-hydroxyphenylalanine in cows with ovarian inactivity provide new directions for research on the mechanism of ovarian inactivity in cows. PMID:27291083

  9. A phytochemical comparison of saw palmetto products using gas chromatography and 1H nuclear magnetic resonance spectroscopy metabolomic profiling

    PubMed Central

    Booker, Anthony; Suter, Andy; Krnjic, Ana; Strassel, Brigitte; Zloh, Mire; Said, Mazlina; Heinrich, Michael

    2014-01-01

    Objectives Preparations containing saw palmetto berries are used in the treatment of benign prostatic hyperplasia (BPH). There are many products on the market, and relatively little is known about their chemical variability and specifically the composition and quality of different saw palmetto products notwithstanding that in 2000, an international consultation paper from the major urological associations from the five continents on treatments for BPH demanded further research on this topic. Here, we compare two analytical approaches and characterise 57 different saw palmetto products. Methods An established method – gas chromatography – was used for the quantification of nine fatty acids, while a novel approach of metabolomic profiling using 1H nuclear magnetic resonance (NMR) spectroscopy was used as a fingerprinting tool to assess the overall composition of the extracts. Key findings The phytochemical analysis determining the fatty acids showed a high level of heterogeneity of the different products in the total amount and of nine single fatty acids. A robust and reproducible 1H NMR spectroscopy method was established, and the results showed that it was possible to statistically differentiate between saw palmetto products that had been extracted under different conditions but not between products that used a similar extraction method. Principal component analysis was able to determine those products that had significantly different metabolites. Conclusions The metabolomic approach developed offers novel opportunities for quality control along the value chain of saw palmetto and needs to be followed further, as with this method, the complexity of a herbal extract can be better assessed than with the analysis of a single group of constituents. PMID:24417505

  10. Computer program TRACK_TEST for calculating parameters and plotting profiles for etch pits in nuclear track materials

    NASA Astrophysics Data System (ADS)

    Nikezic, D.; Yu, K. N.

    2006-01-01

    A computer program called TRACK_TEST for calculating parameters (lengths of the major and minor axes) and plotting profiles in nuclear track materials resulted from light-ion irradiation and subsequent chemical etching is described. The programming steps are outlined, including calculations of alpha-particle ranges, determination of the distance along the particle trajectory penetrated by the chemical etchant, calculations of track coordinates, determination of the lengths of the major and minor axes and determination of the contour of the track opening. Descriptions of the program are given, including the built-in V functions for the two commonly employed nuclear track materials commercially known as LR 115 (cellulose nitrate) and CR-39 (poly allyl diglycol carbonate) irradiated by alpha particles. Program summaryTitle of the program:TRACK_TEST Catalogue identifier:ADWT Program obtainable from:CPC Program Library, Queen's University of Belfast, N. Ireland Program summary URL:http://cpc.cs.qub.ac.uk/summaries/ADWT Computer:Pentium PC Operating systems:Windows 95+ Programming language:Fortran 90 Memory required to execute with typical data:256 MB No. of lines in distributed program, including test data, etc.: 2739 No. of bytes in distributed program, including test data, etc.:204 526 Distribution format:tar.gz External subprograms used:The entire code must be linked with the MSFLIB library Nature of problem: Fast heavy charged particles (like alpha particles and other light ions etc.) create latent tracks in some dielectric materials. After chemical etching in aqueous NaOH or KOH solutions, these tracks become visible under an optical microscope. The growth of a track is based on the simultaneous actions of the etchant on undamaged regions (with the bulk etch rate V) and along the particle track (with the track etch rate V). Growth of the track is described satisfactorily by these two parameters ( V and V). Several models have been presented in the past describing

  11. Partnering for success.

    PubMed

    Gookin, L B

    1996-01-01

    Almost every health care journal or newsletter I pick up has at least one article about the changes occurring in the health care delivery system. The changes for providers in all settings are enormous as we downsize, rightsize, merge, or simply change to meet the demands of managed care. The bottom line is that hospitals are shrinking and health care is moving into the outpatient and community environment. Those of us who are providers in any of these settings must respond rapidly to the changes, which are having a profound impact on the practice of nursing today that will continue into the future. Are nursing educators prepared to respond as quickly as providers to the environmental changes, and are they prepared to make the necessary curriculum changes to prepare students for their future profession? It seems more critical than ever for providers and educators to partner to successfully meet the current and future demands of the nursing profession. PMID:9157919

  12. Intimate Partner Violence. Prevention Update

    ERIC Educational Resources Information Center

    Higher Education Center for Alcohol, Drug Abuse, and Violence Prevention, 2011

    2011-01-01

    The Centers for Disease Control and Prevention (CDC) defines intimate partner violence (IPV) as violence between two people in a close relationship, including current and former spouses and dating partners. IPV occurs on a continuum from a single episode to ongoing battering and can include physical violence, sexual violence, threats, emotional…

  13. WASPs (Wives As Senior Partners).

    ERIC Educational Resources Information Center

    Atkinson, Maxine P.; Boles, Jacqueline

    For the past decade dual-earner marriages in which wives are junior partners have been a major topic in marriage and family literature. In marriages which consider wives as senior partners (WASPs), however, the wife's career is emphasized. In an effort to study the rewards and costs of WASP marriages and to discover the structural conditions under…

  14. Partnering with the NCPV (Brochure)

    SciTech Connect

    Not Available

    2013-06-01

    Brochure that explains the basic partnering opportunities that exist within the National Center for Photovoltaics for industry and university groups: non-proprietary partnering opportunities, competitive solicitations, Technology Partnership Agreements, seed fund to develop Technology Partnership Agreements, Hands-On PV Experience Workshop, and NCPV Fellowship Program.

  15. Quantification of Hydrogen Concentrations in Surface and Interface Layers and Bulk Materials through Depth Profiling with Nuclear Reaction Analysis.

    PubMed

    Wilde, Markus; Ohno, Satoshi; Ogura, Shohei; Fukutani, Katsuyuki; Matsuzaki, Hiroyuki

    2016-01-01

    Nuclear reaction analysis (NRA) via the resonant (1)H((15)N,αγ)(12)C reaction is a highly effective method of depth profiling that quantitatively and non-destructively reveals the hydrogen density distribution at surfaces, at interfaces, and in the volume of solid materials with high depth resolution. The technique applies a (15)N ion beam of 6.385 MeV provided by an electrostatic accelerator and specifically detects the (1)H isotope in depths up to about 2 μm from the target surface. Surface H coverages are measured with a sensitivity in the order of ~10(13) cm(-2) (~1% of a typical atomic monolayer density) and H volume concentrations with a detection limit of ~10(18) cm(-3) (~100 at. ppm). The near-surface depth resolution is 2-5 nm for surface-normal (15)N ion incidence onto the target and can be enhanced to values below 1 nm for very flat targets by adopting a surface-grazing incidence geometry. The method is versatile and readily applied to any high vacuum compatible homogeneous material with a smooth surface (no pores). Electrically conductive targets usually tolerate the ion beam irradiation with negligible degradation. Hydrogen quantitation and correct depth analysis require knowledge of the elementary composition (besides hydrogen) and mass density of the target material. Especially in combination with ultra-high vacuum methods for in-situ target preparation and characterization, (1)H((15)N,αγ)(12)C NRA is ideally suited for hydrogen analysis at atomically controlled surfaces and nanostructured interfaces. We exemplarily demonstrate here the application of (15)N NRA at the MALT Tandem accelerator facility of the University of Tokyo to (1) quantitatively measure the surface coverage and the bulk concentration of hydrogen in the near-surface region of a H2 exposed Pd(110) single crystal, and (2) to determine the depth location and layer density of hydrogen near the interfaces of thin SiO2 films on Si(100). PMID:27077920

  16. Care Partners and Multiple Sclerosis

    PubMed Central

    Quig, Mary Elizabeth; Tyry, Tuula; Marrie, Ruth Ann; Cutter, Gary; Shearin, Edward; Johnson, Kamau; Simsarian, James

    2015-01-01

    Background: Caring for someone with multiple sclerosis (MS) can be a stressful experience that requires clinical attention. We investigated the impact of caregiver stress on the emotional well-being and physical health of the MS care partner using the North American Research Committee on Multiple Sclerosis (NARCOMS) Registry. Methods: Care partners of NARCOMS participants were invited to complete an online questionnaire that captured demographic characteristics, health status, caregiver burden as measured by the Zarit Caregiver Burden Interview, and impact of caregiving on employment. Results: Of 1446 care partners who agreed to participate, 1333 had complete data. Most were men (n = 825, 61.9%), with a mean (SD) age of 51.1 (11.2) years. The mean (SD) Zarit total score was 24.6 (15.1), placing the overall group in the mild caregiver burden range. Compared with male care partners, female care partners reported higher levels of burden and stress and more medication use for stress/anxiety and mood disorders. Male care partners were more likely to report physical concerns. Care partners of people with primary progressive MS reported greater perceived burden than did partners of people with secondary progressive MS and relapsing-remitting MS. More than 40% of care partners (559 of 1288) had missed work during the past year owing to caregiving responsibilities. Conclusions: Care partners of people with MS have substantial physical and psychological health concerns and experience an adverse impact on employment. Future research should evaluate how to mitigate the adverse effects of caregiving and evaluate positive aspects of the role. PMID:26664330

  17. Risk Comparison among Youth Who Report Sex with Same-Sex versus Both-Sex Partners

    ERIC Educational Resources Information Center

    Moon, Martha W.; Fornili, Katherine; O'Briant, Amanda L.

    2007-01-01

    This article examines risk behavior among youth attending support groups for sexual minority youth in Richmond, Virginia, using a structured survey, with particular attention to partner selection and its relationship to risk. Within this generally high-risk group, youth reporting sex partners of both sexes had significantly higher risk profiles,…

  18. Intermolecular protein interactions in solutions of calf lens alpha-crystallin. Results from 1/T1 nuclear magnetic relaxation dispersion profiles.

    PubMed Central

    Koenig, S H; Brown, R D; Spiller, M; Chakrabarti, B; Pande, A

    1992-01-01

    From analyses of the magnetic field dependence of 1/T1 (NMRD profiles) of water protons in solutions of calf lens alpha-crystallin at several concentrations, we find two regimes of solute behavior in both cortical and nuclear preparations. Below approximately 15% vol/vol protein concentration, the solute molecules appear as compact globular proteins of approximately 1,350 (cortical) and approximately 1,700 (nuclear) kD. At higher concentrations, the effective solute particle size increases, reversibly, as evidenced by the appearance of spectra-like 14N peaks in the NMRD profiles and a change in the field and temperature dependence of 1/T1. At these higher concentrations, the profiles are very similar to those of calf gamma II-crystallin, a crystallin that undergoes an analogous transition near approximately 15% protein (Koenig, S. H., C.F. Beaulieu, R. D. Brown III, and M. Spiller, 1990. Biophys. J. 57:461-469). By comparison with recent analyses of NMRD results for solutions of immobilized proteins as models for the transition from protein solutions to tissue (Koenig, S. H., and R. D. Brown III. 1991. Prog. NMR Spectr. 22:487-567), we argue that alpha-crystallin solute behaves as aggregates approximately greater than 50,000 kD as protein concentration is progressively increased above 15%. Finally, the concentration dependence of the NMRD profiles of alpha- and gamma II-crystallin can readily explain recent osmotic pressure data, in particular the intersection of the respective pressure curves at approximately 23% vol/vol (Vérétout, F., and A. Tardieu. 1989. Eur. Biophys. J. 17:61-68). Images FIGURE 1 FIGURE 2 FIGURE 3 FIGURE 4 FIGURE 7 PMID:1504248

  19. Partner Ballroom Dance Robot -PBDR-

    NASA Astrophysics Data System (ADS)

    Kosuge, Kazuhiro; Takeda, Takahiro; Hirata, Yasuhisa; Endo, Mitsuru; Nomura, Minoru; Sakai, Kazuhisa; Koizumi, Mizuo; Oconogi, Tatsuya

    In this research, we have developed a dance partner robot, which has been developed as a platform for realizing the effective human-robot coordination with physical interaction. The robot could estimate the next dance step intended by a human and dance the step with the human. This paper introduce the robot referred to as PBDR (Partner Ballroom Dance Robot), which has performed graceful dancing with the human in EXPO 2005, Aichi, Japan.

  20. Using Smartphone Apps in STD Interviews to Find Sexual Partners

    PubMed Central

    Pennise, Melissa; Herpin, Kate; Owens, John; Bedard, Brenden A.; Weimer, Anita C.; Kennedy, Byron S.; Younge, Mary

    2015-01-01

    Objectives Smartphone applications (apps) are increasingly used to facilitate casual sexual relationships, increasing the risk of sexually transmitted diseases (STDs). In STD investigations, traditional contact elicitation methods can be enhanced with smartphone technology during field interviews. Methods In 2013, the Monroe County Department of Public Health conducted a large, multi-infection STD investigation among men who have sex with men (MSM) using both index case and cluster interviews. When patients indicated meeting sexual partners online, disease intervention specialists (DISs) had access to smartphone apps and were able to elicit partners through access to inboxes and profiles where traditional contact information was lacking. Social network mapping was used to display the extent of the investigation and the impact of access to smartphones on the investigation. Results A total of 14 index patient interviews and two cluster interviews were conducted; 97 individuals were identified among 117 sexual dyads. On average, eight partners were elicited per interview (range: 1–31). The seven individuals who used apps to find partners had an average of three Internet partners (range: 1–5). Thirty-six individuals either had a new STD (n=7) or were previously known to be HIV-positive (n=29). Of the 117 sexual dyads, 21 (18%) originated either online (n=8) or with a smartphone app (n=13). Of those originating online or with a smartphone app, six (29%) partners were located using the smartphone and two (10%) were notified of their exposure via a website. Three of the new STD/HIV cases were among partners who met online. Conclusion Smartphone technology used by DISs in the field improved contact elicitation and resulted in successful partner notification and case finding. PMID:25931628

  1. Partner violence and abortion characteristics.

    PubMed

    Colarossi, Lisa; Dean, Gillian

    2014-01-01

    We conducted a retrospective cohort study using randomly selected medical charts of women reporting a history of partner violence and women with no history of partner violence at the time of a family planning or abortion appointment (n = 6,564 per group). We analyzed lifetime history of partner violence for odds of lifetime history of abortion and miscarriage number, and birth control problems. To more closely match timing, we analyzed a subsample of 2,186 women reporting current violence versus not at the time of an abortion appointment for differences in gestational age, medical versus surgical method choice, and return for follow-up visit. After adjusting for years at risk and demographic characteristics, women with a past history of partner violence were not more likely to have ever had one abortion, but they were more likely to have had problems with birth control, repeat abortions, and miscarriages than women with no history of violence. Women with current partner violence were also more likely to be receiving an abortion at a later gestational age. We found no differences between the groups in return for abortion follow-up visit or choice of surgical versus medication abortion. Findings support screening for the influence of partner violence on reproductive health and related safety planning. PMID:24580133

  2. (1)H-Nuclear magnetic resonance-based metabolic profiling of nonsteroidal anti-inflammatory drug-induced adverse effects in rats.

    PubMed

    Um, So Young; Park, Jung Hyun; Chung, Myeon Woo; Choi, Ki Hwan; Lee, Hwa Jeong

    2016-09-10

    Nonsteroidal anti-inflammatory drugs (NSAIDs), which are globally prescribed, exhibit mainly anti-inflammatory and analgesic effects but also can cause adverse effects including gastrointestinal erosions, ulceration, bleeding, and perforation. The purpose of this study was to investigate surrogate biomarkers associated with the gastrointestinal (GI) damage caused by NSAID treatment using pattern recognition analysis of (1)H-nuclear magnetic resonance ((1)H NMR) spectra of rat urine. Urine was collected for 5h after oral administration of the following NSAIDs at low or high doses: acetylsalicylic acid (10 or 200mgkg(-1)), diclofenac (0.5 or 15mgkg(-1)), piroxicam (1 or 10mgkg(-1)), indomethacin (1 or 25mgkg(-1)), or ibuprofen (10, or 150mgkg(-1)) as nonselective COX inhibitors and celecoxib (10 or 100mgkg(-1)) as a COX-2 selective inhibitor. The urine was analyzed using 500MHz (1)H NMR for spectral binning and targeted profiling and the level of gastric damage was examined. The nonselective COX inhibitors caused severe gastric damage while no lesions were observed in the celecoxib-treated rats. The (1)H NMR urine spectra were divided into spectral bins (0.04ppm) for global profiling, and a total of 44 endogenous metabolites were assigned for targeted profiling. Multivariate data analyses were performed to recognize the spectral pattern of endogenous metabolites related to NSAIDs using partial least square-discrimination analysis (PLS-DA). The (1)H NMR spectra clustered differently according to gastric damage score in global profiling. In targeted profiling, the endogenous metabolites of citrate, allantoin, 2-oxoglutarate, acetate, benzoate, glycine, and trimethylamine N-oxide were selected as putative biomarkers for gastric damage caused by NSAIDs. These putative biomarkers might be useful for predicting the risk of adverse effects caused by NSAIDs in the early stage of drug development process. PMID:27497650

  3. Intimate partner violence, partner notification, and expedited partner therapy: a qualitative study.

    PubMed

    Rosenfeld, Elian A; Marx, John; Terry, Martha A; Stall, Ronald; Pallatino, Chelsea; Borrero, Sonya; Miller, Elizabeth

    2016-07-01

    SummaryOver one-third of women experience intimate partner violence (IPV) in their lifetime. IPV increases the risk of infection and re-infection with sexually transmitted infections (STIs). The extent to which health care providers consider IPV when recommending partner notification and expedited partner therapy is unknown. The objective of this qualitative study was to understand health care providers' views on IPV and STIs when recommending partner treatment to patients with chlamydia. Using a purposive sampling strategy to include health care providers who treat young women at risk for chlamydia, 23 semi-structured, in-depth interviews were conducted. While some health care providers expressed concern for their patients' safety and believed assessing for IPV was needed before provision of expedited partner therapy, nearly a third had not considered the links between IPV and STIs. Strategies used by health care providers to assess for IPV did not include inquiry about specific behaviours related to IPV, STI risk, and sexual coercion. Many health care providers understand the risk for IPV in the setting of STI treatment, yet a significant portion of those interviewed failed to recognise the link between IPV and STIs. Provider education is necessary to increase knowledge and implement more effective inquiry and counselling about IPV to more safely recommend expedited partner therapy. PMID:26088259

  4. Publishing protocols for partnered research.

    PubMed

    Hysong, Sylvia J; Woodard, LeChauncy; Garvin, Jennifer H; Murawsky, Jeffrey; Petersen, Laura A

    2014-12-01

    Published scientific protocols are advocated as a means of controlling bias in research reporting. Indeed, many journals require a study protocol with manuscript submission. However, publishing protocols of partnered research (PPR) can be challenging in light of the research model's dynamic nature, especially as no current reporting standards exist. Nevertheless, as these protocols become more prevalent, a priori documentation of methods in partnered research studies becomes increasingly important. Using as illustration a suite of studies aimed at improving coordination and communication in the primary care setting, we sought to identify challenges in publishing PPR relative to traditional designs, present alternative solutions to PPR publication, and propose an initial checklist of content to be included in protocols of partnered research. Challenges to publishing PPR include reporting details of research components intended to be co-created with operational partners, changes to sampling and entry strategy, and alignment of scientific and operational goals. Proposed solutions include emulating reporting standards of qualitative research, participatory action research, and adaptive trial designs, as well as embracing technological tools that facilitate publishing adaptive protocols, with version histories that are able to be updated as major protocol changes occur. Finally, we present a proposed checklist of reporting elements for partnered research protocols. PMID:25355092

  5. Violence between Therapy-Seeking Veterans and Their Partners: Prevalence and Characteristics of Nonviolent, Mutually Violent, and One-Sided Violent Couples

    ERIC Educational Resources Information Center

    Teten, Andra L.; Sherman, Michelle D.; Han, Xiaotong

    2009-01-01

    Among male veterans and their female partners seeking therapy for relationship issues, three violence profiles were identified based on self-reports of physical violence: nonviolent, in which neither partner reported perpetrating physical violence (44%); one-sided violent, in which one partner reported perpetrating violence (30%); and mutually…

  6. Isotopic signature of selected lanthanides for nuclear activities profiling using cloud point extraction and ICP-MS/MS.

    PubMed

    Labrecque, Charles; Lebed, Pablo J; Larivière, Dominic

    2016-05-01

    The presence of fission products, which include numerous isotopes of lanthanides, can impact the isotopic ratios of these elements in the environment. A cloud point extraction (CPE) method was used as a preconcentration/separation strategy prior to measurement of isotopic ratios of three lanthanides (Nd, Sm, and Eu) by inductively coupled plasma tandem mass spectrometry (ICP-MS/MS). To minimise polyatomic interference, the combination of interferents removal by CPE, reaction/collision cell conditions in He and NH3 mode and tandem quadrupole configuration was investigated and provided optimal results for the determination of isotopic ratio in environmental samples. Isotopic ratios were initially measured in San Joaquin soil (NIST-2709a), an area with little contamination of nuclear origin. Finally, samples collected from three sites with known nuclear activities (Fangataufa Lagoon in French Polynesia, Chernobyl and the Ottawa River near Chalk River Laboratory) were analysed and all exhibited altered isotopic ratios for (143/145)Nd, (147/149)Sm, and (151/153)Eu. These results demonstrate the potential of CPE and ICP-MS/MS for the detection of altered isotopic ratio in environmental samples collected in area subjected to nuclear anthropogenic contamination. The detection of variations in these isotopic ratios of fission products represents the first application of CPE in nuclear forensic investigations of environmental samples. PMID:26895346

  7. Three-dimensional Stellar Kinematics at the Galactic Center: Measuring the Nuclear Star Cluster Spatial Density Profile, Black Hole Mass, and Distance

    NASA Astrophysics Data System (ADS)

    Do, T.; Martinez, G. D.; Yelda, S.; Ghez, A.; Bullock, J.; Kaplinghat, M.; Lu, J. R.; Peter, A. H. G.; Phifer, K.

    2013-12-01

    We present three-dimensional (3D) kinematic observations of stars within the central 0.5 pc of the Milky Way (MW) nuclear star cluster (NSC) using adaptive optics imaging and spectroscopy from the Keck telescopes. Recent observations have shown that the cluster has a shallower surface density profile than expected for a dynamically relaxed cusp, leading to important implications for its formation and evolution. However, the true 3D profile of the cluster is unknown due to the difficulty in de-projecting the stellar number counts. Here, we use spherical Jeans modeling of individual proper motions and radial velocities to constrain, for the first time, the de-projected spatial density profile, cluster velocity anisotropy, black hole mass (M BH), and distance to the Galactic center (R 0) simultaneously. We find that the inner stellar density profile of the late-type stars, ρ(r)vpropr -γ, have a power law slope \\gamma =0.05_{-0.60}^{+0.29}, much more shallow than the frequently assumed Bahcall-Wolf slope of γ = 7/4. The measured slope will significantly affect dynamical predictions involving the cluster, such as the dynamical friction time scale. The cluster core must be larger than 0.5 pc, which disfavors some scenarios for its origin. Our measurement of M_{BH}=5.76_{-1.26}^{+1.76}\\times 10^6 M ⊙ and R_0=8.92_{-0.55}^{+0.58} kpc is consistent with that derived from stellar orbits within 1'' of Sgr A*. When combined with the orbit of S0-2, the uncertainty on R 0 is reduced by 30% (8.46_{-0.38}^{+0.42}\\ kpc). We suggest that the MW NSC can be used in the future in combination with stellar orbits to significantly improve constraints on R 0.

  8. Small Heterodimer Partner and Innate Immune Regulation

    PubMed Central

    Jin, Hyo Sun

    2016-01-01

    The nuclear receptor superfamily consists of the steroid and non-steroid hormone receptors and the orphan nuclear receptors. Small heterodimer partner (SHP) is an orphan family nuclear receptor that plays an essential role in the regulation of glucose and cholesterol metabolism. Recent studies reported a previously unidentified role for SHP in the regulation of innate immunity and inflammation. The innate immune system has a critical function in the initial response against a variety of microbial and danger signals. Activation of the innate immune response results in the induction of inflammatory cytokines and chemokines to promote anti-microbial effects. An excessive or uncontrolled inflammatory response is potentially harmful to the host, and can cause tissue damage or pathological threat. Therefore, the innate immune response should be tightly regulated to enhance host defense while preventing unwanted immune pathologic responses. In this review, we discuss recent studies showing that SHP is involved in the negative regulation of toll-like receptor-induced and NLRP3 (NACHT, LRR and PYD domains-containing protein 3)-mediated inflammatory responses in innate immune cells. Understanding the function of SHP in innate immune cells will allow us to prevent or modulate acute and chronic inflammation processes in cases where dysregulated innate immune activation results in damage to normal tissues. PMID:26754583

  9. Small Heterodimer Partner and Innate Immune Regulation.

    PubMed

    Yuk, Jae Min; Jin, Hyo Sun; Jo, Eun Kyeong

    2016-03-01

    The nuclear receptor superfamily consists of the steroid and non-steroid hormone receptors and the orphan nuclear receptors. Small heterodimer partner (SHP) is an orphan family nuclear receptor that plays an essential role in the regulation of glucose and cholesterol metabolism. Recent studies reported a previously unidentified role for SHP in the regulation of innate immunity and inflammation. The innate immune system has a critical function in the initial response against a variety of microbial and danger signals. Activation of the innate immune response results in the induction of inflammatory cytokines and chemokines to promote anti-microbial effects. An excessive or uncontrolled inflammatory response is potentially harmful to the host, and can cause tissue damage or pathological threat. Therefore, the innate immune response should be tightly regulated to enhance host defense while preventing unwanted immune pathologic responses. In this review, we discuss recent studies showing that SHP is involved in the negative regulation of toll-like receptor-induced and NLRP3 (NACHT, LRR and PYD domains-containing protein 3)-mediated inflammatory responses in innate immune cells. Understanding the function of SHP in innate immune cells will allow us to prevent or modulate acute and chronic inflammation processes in cases where dysregulated innate immune activation results in damage to normal tissues. PMID:26754583

  10. 2H nuclear magnetic resonance order parameter profiles suggest a change of molecular shape for phosphatidylcholines containing a polyunsaturated acyl chain.

    PubMed Central

    Holte, L. L.; Peter, S. A.; Sinnwell, T. M.; Gawrisch, K.

    1995-01-01

    Solid-state 2H nuclear magnetic resonance spectroscopy was used to determine the orientational order parameter profiles for a series of phosphatidylcholines with perdeuterated stearic acid, 18:0d35, in position sn-1 and 18:1 omega 9, 18:2 omega 6, 18:3 omega 3, 20:4 omega 6, 20:5 omega 3, or 22:6 omega 3 in position sn-2. The main phase transition temperatures were derived from a first moment analysis, and order parameter profiles of sn-1 chains were calculated from dePaked nuclear magnetic resonance powder patterns. Comparison of the profiles at 37 degrees C showed that unsaturation causes an inhomogenous disordering along the sn-1 chain. Increasing sn-2 chain unsaturation from one to six double bonds resulted in a 1.6-kHz decrease in quadrupolar splittings of the sn-1 chain in the upper half of the chain (or plateau region) and maximum splitting difference of 4.4 kHz at methylene carbon 14. The change in chain order corresponds to a decrease in the 18:0 chain length of 0.4 +/- 0.2 A with 18:2 omega 6 versus 18:1 omega 9 in position sn-2. Fatty acids containing three or more double bonds in sn-2 showed a decrease in sn-1 chain length of 0.7 +/- 0.2 A compared with 18:1 omega 9. The chain length of all lipids decreased with increasing temperature. Highly unsaturated phosphatidylcholines (three or more double bonds in sn-2) had shorter sn-1 chains, but the chain length was somewhat less sensitive to temperature. The profiles reveal that the sn-1 chain exhibits a selective increase in motional freedom in a region located toward the bottom half of the chain as sn-2 unsaturation is increased. This corresponds to an area increase around carbon atom number 14 that is three to four times greater than the increase for the top part of the chain. A similar asymmetric decrease in order, largest toward the methyl end of the chain, was observed when 1 -palmitoyl-2-oleoylphosphatidylethanolamine goes from a lamellar to an inverse hexagonal (H,,) phase. This is consistent with a

  11. Tetrapyrrole profiling in Arabidopsis seedlings reveals that retrograde plastid nuclear signaling is not due to Mg-protoporphyrin IX accumulation

    PubMed Central

    Moulin, Michael; McCormac, Alex C.; Terry, Matthew J.; Smith, Alison G.

    2008-01-01

    Chloroplast biogenesis involves careful coordination of both plastid and nuclear gene expression, which is achieved in part by retrograde signaling from the chloroplast to the nucleus. This can be demonstrated by the fact that the herbicide, Norflurazon (NF), which causes bleaching of chloroplasts, prevents the light induction of photosynthesis-related genes in the nucleus. It has been proposed that the tetrapyrrole pathway intermediate Mg-protoporphyrin IX acts as the signaling molecule in this pathway and accumulates in the chloroplasts and cytosol of the cell after NF treatment. Here we present data that demonstrate that this model is too simplistic. We have developed a sensitive liquid chromatography-mass spectrometry (LC/MS) method to measure tetrapyrrole intermediates and have shown that no Mg-protoporphyrin IX, nor indeed any other chlorophyll-biosynthesis intermediate, can be detected in NF-treated plants under conditions in which nuclear gene expression is repressed. Conversely when endogenous Mg-protoporphyrin IX levels are artificially increased by supplementation with the tetrapyrrole precursor, 5-aminolevulinic acid, the expression of nuclear-encoded photosynthetic genes is induced, not repressed. We also demonstrate that NF-treatment leads to a strong down-regulation of tetrapyrrole biosynthesis genes, consistent with the absence of an accumulation of tetrapyrrole intermediates. Finally, there is no correlation between nuclear-gene expression and any of the chlorophyll biosynthetic intermediates over a range of growth conditions and treatments. Instead, it is possible that a perturbation of tetrapyrrole synthesis may lead to localized ROS production or an altered redox state of the plastid, which could mediate retrograde signaling. PMID:18818314

  12. The Symmetry of Partner Modelling

    ERIC Educational Resources Information Center

    Dillenbourg, Pierre; Lemaignan, Séverin; Sangin, Mirweis; Nova, Nicolas; Molinari, Gaëlle

    2016-01-01

    Collaborative learning has often been associated with the construction of a shared understanding of the situation at hand. The psycholinguistics mechanisms at work while establishing common grounds are the object of scientific controversy. We postulate that collaborative tasks require some level of mutual modelling, i.e. that each partner needs…

  13. The Computer as Lab Partner.

    ERIC Educational Resources Information Center

    Nicklin, R. C.

    1985-01-01

    Microcomputers can record laboratory measurements which human laboratory partners can never collect. Simple, harder, and general-purpose interfaces are discussed, with suggestions for several experiments involving an exercise bike, acceleration, and pendulums. Additional applications with pH meters, spectrophotometers, and chromatographs are also…

  14. OLC Partners with the People.

    ERIC Educational Resources Information Center

    Dellinger, Laura M.

    2001-01-01

    Discusses ways in which Oglala Lakota College (South Dakota) helps to strengthen the cultural fabric of the Lakota community and how it has partnered with other institutions of higher learning throughout the state. Reports that the college has a number of community-based initiatives that emphasize the relationship between academics and ancestral…

  15. Macomb Reading Partners Research Report.

    ERIC Educational Resources Information Center

    Snoddy, James E.

    A study evaluated Macomb Reading Partners (MRP), the tutoring program of the Macomb Literacy Project. It researched the effectiveness of literacy training and its impact on the lives of a target group of 30 automotive workers. Data sources were a structured interview, the Word Opposites Test of the Botel Reading Inventory (1962), and participant…

  16. Partnering for Student Transfer Success

    ERIC Educational Resources Information Center

    Washington State Board for Community and Technical Colleges, 2015

    2015-01-01

    Washington's community and technical colleges, private non-profit baccalaureate, and public baccalaureate colleges and universities have a proud history of partnering to serve students. Nowhere is this cooperation more evident than in the smooth transfer process from community and technical colleges into four-year colleges and universities. This…

  17. Partner Teaching: A Promising Model

    ERIC Educational Resources Information Center

    Bronson, Carroll E.; Dentith, Audrey M.

    2014-01-01

    This paper describes an ethnographic case study of a partner or co-teaching classroom in an urban preschool classroom. As part of a larger project that evaluated classroom size and team teaching structures in Kindergarten classrooms in several high poverty urban schools, one successful co-teaching classroom was studied further. Systematic…

  18. Measuring radial profiles of nuclear burn in ICF implosions at OMEGA and the NIF using proton emission imaging

    NASA Astrophysics Data System (ADS)

    Seguin, F. H.; Rinderknecht, H. G.; Rosenberg, M.; Zylstra, A.; Frenje, J.; Li, C. K.; Petrasso, R.; Marshall, F. J.; Sangster, T. C.; Hoffman, N. M.; Amendt, P. A.; Bellei, C.; Le Pape, S.; Wilks, S. C.

    2014-10-01

    Fusion reactions in ICF implosions of D3He-filled capsules produce 14.7-MeV D3He protons and 3-MeV DD protons. Measurements of the spatial distributions of the D3He and DD reactions are studied with a penumbral imaging system that utilizes a CR-39-based imaging detector to simultaneously record separate penumbral images of the two types of protons. Measured burn profiles are useful for studying implosion physics and provide a critical test for benchmarking simulations. Recent implosions at OMEGA of CD capsules containing 3He gas fill and SiO2 capsules containing low-pressure D3He gas were expected to have hollow D3He burn profiles (in the 3He-filled capsule, due to fuel-shell mix), but penumbral imaging showed that the reactions were centrally peaked due to enhanced ion diffusion. The imaging technique is to be implemented soon on the NIF. This work was supported in part by NLUF, DOE, and LLE.

  19. Measuring the stellar luminosity function and spatial density profile of the inner 0.5 pc of the Milky Way nuclear star cluster

    NASA Astrophysics Data System (ADS)

    Do, Tuan; Ghez, Andrea; Lu, Jessica R.; Morris, Mark R.; Yelda, Sylvana; Martinez, Gregory D.; Peter, Annika H. G.; Wright, Shelley; Bullock, James; Kaplinghat, Manoj; Matthews, K.

    2012-07-01

    We report on measurements of the luminosity function of early (young) and late-type (old) stars in the central 0.5 pc of the Milky Way nuclear star cluster as well as the density profiles of both components. The young (~ 6 Myr) and old stars (> 1 Gyr) in this region provide different physical probes of the environment around a supermassive black hole; the luminosity function of the young stars offers us a way to measure the initial mass function from star formation in an extreme environment, while the density profile of the old stars offers us a probe of the dynamical interaction of a star cluster with a massive black hole. The two stellar populations are separated through a near-infrared spectroscopic survey using the integral-field spectrograph OSIRIS on Keck II behind the laser guide star adaptive optics system. This spectroscopic survey is able to separate early-type (young) and late-type (old) stars with a completeness of 50% at K' = 15.5. We describe our method of completeness correction using a combination of star planting simulations and Bayesian inference. The completeness corrected luminosity function of the early-type stars contains significantly more young stars at faint magnitudes compared to previous surveys with similar depth. In addition, by using proper motion and radial velocity measurements along with anisotropic spherical Jeans modeling of the cluster, it is possible to measure the spatial density profile of the old stars, which has been difficult to constrain with number counts alone. The most probable model shows that the spatial density profile, n(r) propto r-γ, to be shallow with γ = 0.4 ± 0.2, which is much flatter than the dynamically relaxed case of γ = 3/2 to 7/4, but does rule out a 'hole' in the distribution of old stars. We show, for the first time, that the spatial density profile, the black hole mass, and velocity anisotropy can be fit simultaneously to obtain a black hole mass that is consistent with that derived from

  20. Nuclear APC.

    PubMed

    Neufeld, Kristi L

    2009-01-01

    Mutational inactivation of the tumor suppressor gene APC (Adenomatous polyposis coli) is thought to be an initiating step in the progression of the vast majority ofcolorectal cancers. Attempts to understand APC function have revealed more than a dozen binding partners as well as several subcellular localizations including at cell-cell junctions, associated with microtubules at the leading edge of migrating cells, at the apical membrane, in the cytoplasm and in the nucleus. The present chapter focuses on APC localization and functions in the nucleus. APC contains two classical nuclear localization signals, with a third domain that can enhance nuclear import. Along with two sets of nuclear export signals, the nuclear localization signals enable the large APC protein to shuttle between the nucleus and cytoplasm. Nuclear APC can oppose beta-catenin-mediated transcription. This down-regulation of nuclear beta-catenin activity by APC most likely involves nuclear sequestration of beta-catenin from the transcription complex as well as interaction of APC with transcription corepressor CtBP. Additional nuclear binding partners for APC include transcription factor activator protein AP-2alpha, nuclear export factor Crm1, protein tyrosine phosphatase PTP-BL and perhaps DNA itself. Interaction of APC with polymerase beta and PCNA, suggests a role for APC in DNA repair. The observation that increases in the cytoplasmic distribution of APC correlate with colon cancer progression suggests that disruption of these nuclear functions of APC plays an important role in cancer progression. APC prevalence in the cytoplasm of quiescent cells points to a potential function for nuclear APC in control of cell proliferation. Clear definition of APC's nuclear function(s) will expand the possibilities for early colorectal cancer diagnostics and therapeutics targeted to APC. PMID:19928349

  1. Molecular Profiling of Giant Cell Tumor of Bone and the Osteoclastic Localization of Ligand for Receptor Activator of Nuclear Factor κB

    PubMed Central

    Morgan, Teresa; Atkins, Gerald J.; Trivett, Melanie K.; Johnson, Sandra A.; Kansara, Maya; Schlicht, Stephen L.; Slavin, John L.; Simmons, Paul; Dickinson, Ian; Powell, Gerald; Choong, Peter F.M.; Holloway, Andrew J.; Thomas, David M.

    2005-01-01

    Giant cell tumor of bone (GCT) is a generally benign, osteolytic neoplasm comprising stromal cells and osteoclast-like giant cells. The osteoclastic cells, which cause bony destruction, are thought to be recruited from normal monocytic pre-osteoclasts by stromal cell expression of the ligand for receptor activator of nuclear factor κB (RANKL). This model forms the foundation for clinical trials in GCTs of novel cancer therapeutics targeting RANKL. Using expression profiling, we identified both osteoblast and osteoclast signatures within GCTs, including key regulators of osteoclast differentiation and function such as RANKL, a C-type lectin, osteoprotegerin, and the wnt inhibitor SFRP4. After ex vivo generation of stromal- and osteoclast-enriched cultures, we unexpectedly found that RANKL mRNA and protein were more highly expressed in osteoclasts than in stromal cells, as determined by expression profiling, flow cytometry, immunohistochemistry, and reverse transcriptase-polymerase chain reaction. The expression patterns of molecules implicated in signaling between stromal cells and monocytic osteoclast precursors were analyzed in both primary and fractionated GCTs. Finally, using array-based comparative genomic hybridization, neither GCTs nor the derived stromal cells demonstrated significant genomic gains or losses. These data raise questions regarding the role of RANKL in GCTs that may be relevant to the development of molecularly targeted therapeutics for this disease. PMID:15972958

  2. Nuclear transcriptome profiling of induced pluripotent stem cells and embryonic stem cells identify non-coding loci resistant to reprogramming

    PubMed Central

    Fort, Alexandre; Yamada, Daisuke; Hashimoto, Kosuke; Koseki, Haruhiko; Carninci, Piero

    2015-01-01

    Identification of functionally relevant differences between induced pluripotent stem cells (iPSC) and reference embryonic stem cells (ESC) remains a central question for therapeutic applications. Differences in gene expression between iPSC and ESC have been examined by microarray and more recently with RNA-SEQ technologies. We here report an in depth analyses of nuclear and cytoplasmic transcriptomes, using the CAGE (cap analysis of gene expression) technology, for 5 iPSC clones derived from mouse lymphocytes B and 3 ESC lines. This approach reveals nuclear transcriptomes significantly more complex in ESC than in iPSC. Hundreds of yet not annotated putative non-coding RNAs and enhancer-associated transcripts specifically transcribed in ESC have been detected and supported with epigenetic and chromatin-chromatin interactions data. We identified super-enhancers transcriptionally active specifically in ESC and associated with genes implicated in the maintenance of pluripotency. Similarly, we detected non-coding transcripts of yet unknown function being regulated by ESC specific super-enhancers. Taken together, these results demonstrate that current protocols of iPSC reprogramming do not trigger activation of numerous cis-regulatory regions. It thus reinforces the need for already suggested deeper monitoring of the non-coding transcriptome when characterizing iPSC clones. Such differences in regulatory transcript expression may indeed impact their potential for clinical applications. PMID:25664506

  3. The uterine and vascular actions of estetrol delineate a distinctive profile of estrogen receptor α modulation, uncoupling nuclear and membrane activation

    PubMed Central

    Abot, Anne; Fontaine, Coralie; Buscato, Mélissa; Solinhac, Romain; Flouriot, Gilles; Fabre, Aurélie; Drougard, Anne; Rajan, Shyamala; Laine, Muriel; Milon, Alain; Muller, Isabelle; Henrion, Daniel; Adlanmerini, Marine; Valéra, Marie-Cécile; Gompel, Anne; Gerard, Céline; Péqueux, Christel; Mestdagt, Mélanie; Raymond-Letron, Isabelle; Knauf, Claude; Ferriere, François; Valet, Philippe; Gourdy, Pierre; Katzenellenbogen, Benita S; Katzenellenbogen, John A; Lenfant, Françoise; Greene, Geoffrey L; Foidart, Jean-Michel; Arnal, Jean-François

    2014-01-01

    Estetrol (E4) is a natural estrogen with a long half-life produced only by the human fetal liver during pregnancy. The crystal structures of the estrogen receptor α (ERα) ligand-binding domain bound to 17β-estradiol (E2) and E4 are very similar, as well as their capacity to activate the two activation functions AF-1 and AF-2 and to recruit the coactivator SRC3. In vivo administration of high doses of E4 stimulated uterine gene expression, epithelial proliferation, and prevented atheroma, three recognized nuclear ERα actions. However, E4 failed to promote endothelial NO synthase activation and acceleration of endothelial healing, two processes clearly dependent on membrane-initiated steroid signaling (MISS). Furthermore, E4 antagonized E2 MISS-dependent effects in endothelium but also in MCF-7 breast cancer cell line. This profile of ERα activation by E4, uncoupling nuclear and membrane activation, characterizes E4 as a selective ER modulator which could have medical applications that should now be considered further. PMID:25214462

  4. Comprehensive profiling and marker identification in non-volatile citrus oil residues by mass spectrometry and nuclear magnetic resonance.

    PubMed

    Marti, Guillaume; Boccard, Julien; Mehl, Florence; Debrus, Benjamin; Marcourt, Laurence; Merle, Philippe; Delort, Estelle; Baroux, Lucie; Sommer, Horst; Rudaz, Serge; Wolfender, Jean-Luc

    2014-05-01

    The detailed characterization of cold-pressed lemon oils (CPLOs) is of great importance for the flavor and fragrance (F&F) industry. Since a control of authenticity by standard analytical techniques can be bypassed using elaborated adulterated oils to pretend a higher quality, a combination of advanced orthogonal methods has been developed. The present study describes a combined metabolomic approach based on UHPLC-TOF-MS profiling and (1)H NMR fingerprinting to highlight metabolite differences on a set of representative samples used in the F&F industry. A new protocol was set up and adapted to the use of CPLO residues. Multivariate analysis based on both fingerprinting methods showed significant chemical variations between Argentinian and Italian samples. Discriminating markers identified in mixtures belong to furocoumarins, flavonoids, terpenoids and fatty acids. Quantitative NMR revealed low citropten and high bergamottin content in Italian samples. The developed metabolomic approach applied to CPLO residues gives some new perspectives for authenticity assessment. PMID:24360445

  5. The Role of Character Strengths in Adolescent Romantic Relationships: An Initial Study on Partner Selection and Mates' Life Satisfaction

    ERIC Educational Resources Information Center

    Weber, Marco; Ruch, Willibald

    2012-01-01

    The present study investigated the role of 24 character strengths in 87 adolescent romantic relationships focusing on their role in partner selection and their role in mates' life satisfaction. Measures included the Values in Action Inventory of Strengths for Youth, the Students' Life Satisfaction Scale, and an Ideal Partner Profiler for the…

  6. Talking to Your Partner about Condoms

    MedlinePlus

    ... at the top of your (or your partner's) penis. This gets rid of trapped air, which can ... Remove the condom immediately after ejaculation, before the penis softens. You or your partner should hold the ...

  7. For Better Skin Cancer Checks, Partner Up

    MedlinePlus

    ... gov/medlineplus/news/fullstory_159632.html For Better Skin Cancer Checks, Partner Up Melanoma survivors benefited when ... out: Getting a partner trained to spot potential skin cancers can be a lifesaver for melanoma survivors, ...

  8. Chemical recognition of partner plant species by foundress ant queens in Macaranga-Crematogaster myrmecophytism.

    PubMed

    Inui, Y; Itioka, T; Murase, K; Yamaoka, R; Itino, T

    2001-10-01

    The partnership in the Crematogaster-Macaranga ant-plant interaction is highly species-specific. Because a mutualistic relationship on a Macaranga plant starts with colonization by a foundress queen of a partner Crematogaster species, we hypothesized that the foundress queens select their partner plant species by chemical recognition. We tested this hypothesis with four sympatric Macaranga species and their Crematogaster plant-ant species. We demonstrated that foundress Crematogaster queens can recognize their partner Macaranga species by contact with the surface of the seedlings, that they can recognize compounds from the stem surface of seedlings of their partner plant species, and that the gas chromatographic profiles are characteristic of the plant species. These findings support the hypothesis that foundress queens of the Crematogaster plant-ant species select their partner Macaranga species by recognizing nonvolatile chemical characteristics of the stem surfaces of seedlings. PMID:11710609

  9. Dilemmas in intimate partner violence.

    PubMed

    Cook, Rebecca J; Dickens, Bernard M

    2009-07-01

    Intimate partner violence (IPV), usually men's violence against women, appears universal. It may be associated with pregnancy, but this may be because pregnant women receive more medical attention. Violence may cause bruises, abrasions, and cuts, but its extremes include hospitalization, death, and suicide. IPV is often disclosed when women are asked why they feel in poor health or depressed. A legal dilemma arises when healthcare providers consider that intervention such as law-enforcement is appropriate, but patients refuse approval. Patients may fatalistically accept violence, or fear loss of support for their children and themselves if their partners are held in custody. Legal reforms, such as punishing spousal rape, may provide some protection of women's autonomy. Ethical dilemmas concern intervention without patients' approval, and whether treating violent injuries without taking preventive action breaches the principle to Do No Harm. Professional advocacy and social action have been urged to expose and reduce IPV. PMID:19368921

  10. Partner attachment and interpersonal characteristics.

    PubMed

    Kilmann, Peter R; Finch, Holmes; Parnell, Michele M; Downer, Jason T

    2013-01-01

    This study investigated partner attachment and interpersonal characteristics in 134 nonclinical couples in long-term marriages. Irrespective of gender, spouses with greater anxiety over abandonment or discomfort with closeness endorsed dysfunctional relationship beliefs to a greater extent. On the anxiety over abandonment dimension, husbands with higher scores were rated less aggressive, less controlling, and more rebellious, whereas wives with higher scores were rated more dependent, more self-critical, and less competitive. Husbands higher on discomfort with closeness were rated less cooperative and responsible and were rated more aggressive and rebellious. Matched secure couples reported lower marital dissatisfaction than matched insecure or mismatched couples. Future research should contrast samples of nonclinical and clinical couples by marital duration to identify specific partner behaviors that are likely to foster marital dissatisfaction within particular attachment pairings. The authors' findings suggest the importance of marital therapists being attuned to the attachment-related beliefs and interpersonal styles uniquely operating within each couple. PMID:23252639

  11. Partnering: The foundation for performance

    SciTech Connect

    Edelman, L.

    1994-12-31

    Over the past several decades, the US has experienced an unprecedented increase in the number of disputes in litigation. The environmental area, particularly contracts involving remediation projects, have not been immune from this situation. The adverse impact of this litigation mindset on the financial and personnel resources of the contracting parties has escalated and the detrimental effect on contractual relationships has become more apparent. Both owners and contractors are confronted with unrealized contract expectations, hostility on the jobsite and disappointment in the traditional dispute resolution processes. Relying exclusively on litigation when negotiations fail to settle contract disputes is time consuming, costly and frustrating. Now in the 1990s, the Corps along with the construction industry are moving to establish a new relationship beneficial to all contracting parties that focus on disputes avoidance. This new relationship is fostered by a process called Partnering. In undertaking environmental remediation work, Partnering can lay the foundation for a successful relationship among all involved parties.

  12. Light 't Hooft top partners

    NASA Astrophysics Data System (ADS)

    Cacciapaglia, Giacomo; Parolini, Alberto

    2016-04-01

    Vectorlike quarks, usually dubbed top partners, are a common presence in composite Higgs models. Being composite objects, their mass is expected to be of the order of their inverse size, that is the condensation scale of the new strong interactions. Light top partners, while not being a generic prediction, are, however, often considered in phenomenological models. We suggest that their lightness may be due to the matching of global 't Hooft anomalies of the underlying theory. We check this mechanism in explicit models, showing that, in one case, composite fermions with the quantum numbers of the top quark obtain a mass which is controlled by a soft breaking term and can be made parametrically small.

  13. THREE-DIMENSIONAL STELLAR KINEMATICS AT THE GALACTIC CENTER: MEASURING THE NUCLEAR STAR CLUSTER SPATIAL DENSITY PROFILE, BLACK HOLE MASS, AND DISTANCE

    SciTech Connect

    Do, T.; Martinez, G. D.; Bullock, J.; Kaplinghat, M.; Peter, A. H. G.; Yelda, S.; Ghez, A.; Phifer, K.; Lu, J. R.

    2013-12-10

    We present three-dimensional (3D) kinematic observations of stars within the central 0.5 pc of the Milky Way (MW) nuclear star cluster (NSC) using adaptive optics imaging and spectroscopy from the Keck telescopes. Recent observations have shown that the cluster has a shallower surface density profile than expected for a dynamically relaxed cusp, leading to important implications for its formation and evolution. However, the true 3D profile of the cluster is unknown due to the difficulty in de-projecting the stellar number counts. Here, we use spherical Jeans modeling of individual proper motions and radial velocities to constrain, for the first time, the de-projected spatial density profile, cluster velocity anisotropy, black hole mass (M {sub BH}), and distance to the Galactic center (R {sub 0}) simultaneously. We find that the inner stellar density profile of the late-type stars, ρ(r)∝r {sup –γ}, have a power law slope γ=0.05{sub −0.60}{sup +0.29}, much more shallow than the frequently assumed Bahcall-Wolf slope of γ = 7/4. The measured slope will significantly affect dynamical predictions involving the cluster, such as the dynamical friction time scale. The cluster core must be larger than 0.5 pc, which disfavors some scenarios for its origin. Our measurement of M{sub BH}=5.76{sub −1.26}{sup +1.76}×10{sup 6} M {sub ☉} and R{sub 0}=8.92{sub −0.55}{sup +0.58} kpc is consistent with that derived from stellar orbits within 1'' of Sgr A*. When combined with the orbit of S0-2, the uncertainty on R {sub 0} is reduced by 30% (8.46{sub −0.38}{sup +0.42} kpc). We suggest that the MW NSC can be used in the future in combination with stellar orbits to significantly improve constraints on R {sub 0}.

  14. Desistance From Intimate Partner Violence

    PubMed Central

    Bowen, Erica; Brown, Sarah; Sleath, Emma

    2015-01-01

    Intimate partner violence (IPV) is an international issue that social and criminal justice workers will encounter regularly. It has been identified that men can, and do stop using, or desist from, IPV although it is unclear how this process of change develops. This article introduces a conceptual model to outline how the process of desistance evolves and what it encompasses. Using thematic analysis of interview data from partner-violent men, survivors, and treatment facilitators, the resulting model demonstrates that the process of change is a dynamic one where men’s use of, and cessation from, violence needs to be understood within the context of each individual’s life. Three global themes were developed: (a) lifestyle behaviors (violent): what is happening in the men’s lives when they use violence; (b) catalysts for change: the triggers and transitions required to initiate the process of change; and (c) lifestyle behaviors (non-violent): what is different in the men’s lives when they have desisted from IPV. The purpose of this model is to offer a framework for service providers to assist them to manage the process of change in partner-violent men. PMID:25315483

  15. Adult Attachment as a Risk Factor for Intimate Partner Violence : The "Mispairing" of Partners' Attachment Styles

    ERIC Educational Resources Information Center

    Doumas, Diana M.; Pearson, Christine L.; Elgin, Jenna E.; McKinley, Lisa L.

    2008-01-01

    This study examined the relationship between intimate partner violence and adult attachment in a sample of 70 couples. The attachment style of each partner and the interaction of the partners' attachment styles were examined as predictors of intimate partner violence. Additional analyses were conducted to examine violence reciprocity and to…

  16. Adamantyl-Substituted Retinoid-Derived Molecules That Interact with the Orphan Nuclear Receptor Small Heterodimer Partner: Effects of Replacing the 1-Adamantyl or Hydroxyl Group on Inhibition of Cancer Cell Growth, Induction of Cancer Cell Apoptosis, and Inhibition of Src Homology 2 Domain-Containing Protein Tyrosine Phosphatase-2 Activity

    PubMed Central

    Dawson, Marcia I.; Xia, Zebin; Jiang, Tao; Ye, Mao; Fontana, Joseph A.; Farhana, Lulu; Patel, Bhaumik; Xue, Li Ping; Bhuiyan, Mohammad; Pellicciari, Roberto; Macchiarulo, Antonio; Nuti, Roberto; Zhang, Xiao-Kun; Han, Young-Hoon; Tautz, Lutz; Hobbs, Peter D.; Jong, Ling; Waleh, Nahid; Chao, Wan-ru; Feng, Gen-Sheng; Pang, Yuhong; Su, Ying

    2014-01-01

    (E)-4-[3-(1-Adamantyl)-4′-hydroxyphenyl]-3-chlorocinnamic acid (3-Cl-AHPC) induces the cell-cycle arrest and apoptosis of leukemia and cancer cells. Studies demonstrated that 3-Cl-AHPC bound to the atypical orphan nuclear receptor small heterodimer partner (SHP). Although missing a DNA-binding domain, SHP heterodimerizes with the ligand-binding domains of other nuclear receptors to repress their abilities to induce or inhibit gene expression. 3-Cl-AHPC analogues having the 1-adamantyl and phenolic hydroxyl pharmacophoric elements replaced with isosteric groups were designed, synthesized, and evaluated for their inhibition of proliferation and induction of human cancer cell apoptosis. Structure–anticancer activity relationship studies indicated the importance of both groups to apoptotic activity. Docking of 3-Cl-AHPC and its analogues to an SHP computational model that was based on the crystal structure of ultraspiracle complexed with 1-stearoyl-2-palmitoylglycero-3-phosphoethanolamine suggested why these 3-Cl-AHPC groups could influence SHP activity. Inhibitory activity against Src homology 2 domain-containing protein tyrosine phosphatase 2 (Shp-2) was also assessed. The most active Shp-2 inhibitor was found to be the 3′-(3,3-dimethylbutynyl) analogue of 3-Cl-AHPC. PMID:18759424

  17. Partner selection in the mycorrhizal mutualism.

    PubMed

    Werner, Gijsbert D A; Kiers, E Toby

    2015-03-01

    Partner selection in the mycorrhizal symbiosis is thought to be a key factor stabilising the mutualism. Both plant hosts and mycorrhizal fungi have been shown to preferentially allocate resources to higher quality partners. This can help maintain underground cooperation, although it is likely that different plant species vary in the spatial precision with which they can select partners. Partner selection in the mycorrhizal symbiosis is presumably context-dependent and can be mediated by factors like (relative) resource abundance and resource fluctuations, competition among mycorrhizas, arrival order and cultivation history. Such factors complicate our current understanding of the importance of partner selection and its effectiveness in stimulating mutualistic cooperation. PMID:25421912

  18. Maternal Re-Partnering and New-Partner Fertility: Associations with Nonresident Father Investments in Children

    PubMed Central

    Berger, Lawrence M.; Cancian, Maria; Meyer, Daniel R.

    2011-01-01

    Research suggests that paternal re-partnering and new-partner fertility are associated with decreased nonresident father investments in children. Few studies, however, have examined the influence of maternal re-partnering and new-partner births on nonresident father investments. We use data from the National Longitudinal Survey of Youth to examine associations of maternal re-partnering (through cohabitation or marriage with a new partner) and new-partner births with nonresident father visitation and child support payments. Results suggest that maternal re-partnering is associated with a decrease in both yearly father-child contact and child support received by the mother. New-partner fertility for mothers who are co-residing with a partner is associated with an additional decrease in monthly father-child contact, but does not have an additional influence on yearly father-child contact or child support receipt. PMID:22581998

  19. Depth Profiling of N and C in Ion Implanted ZnO and Si Using Deuterium Induced Nuclear Reaction Analysis

    SciTech Connect

    Kennedy, John; Murmu, Peter; Markwitz, Andreas

    2008-11-03

    Nuclear Reaction Analysis (NRA) with deuteron ion beams has been used to probe for ion implanted nitrogen and carbon with high sensitivity in zinc oxide and silicon single crystals. The ion implanted N was measured using 1.4 MeV deuteron ion beams and was found to be in agreement with calculated values. The limit of detection for N in ZnO is 8x10{sup 14} ions cm{sup -2}. Raman measurements of the ion implanted samples showed three additional modes at 275, 504, and 644 cm{sup -1} compared to the un-implanted ZnO crystals. The NRA and Raman results provided information on the N concentration, depth distribution, and structural changes that occur in dependence on the nitrogen ion fluences. The deuterium induced {sup 12}C(d,p){sup 13}C reaction was used to measure the carbon impurity/dose in ion implanted silicon. It was found that the use of a large cold shield (liquid nitrogen trap) in the ion implanter chamber greatly reduces the amount of carbon impurity on the surface of ion implanted silicon. Various implantations with N{sub 2}, O{sub 2}, NO, NO{sub 2} and Pb ions were performed with and without cooling of the liquid nitrogen trap. Simultaneous detection of ppm-level concentrations of {sup 12}C, {sup 16}O and {sup 14}N enables highly sensitive measurement of impurities that may be incorporated during the fabrication process, transport of the samples and/or storage of the samples in air.

  20. Profiles of nuclear and mitochondrial encoded mRNAs in developing and quiescent embryos of Artemia franciscana.

    PubMed

    Hardewig, I; Anchordoguy, T J; Crawford, D L; Hand, S C

    1996-05-24

    Embryos of the brine shrimp Artemia franciscana are able to withstand long bouts of environmental anoxia by entering a quiescent state during which metabolism is greatly depressed. Recent evidence supports a global arrest of protein synthesis during quiescence. In this study we measured the amounts of mRNA for a mitochondrial-encoded subunit of cytochrome c oxidase (COX I) and for nuclear-encoded actin during aerobic development, anaerobiosis, and aerobic acidosis (artificial quiescence imposed by intracellular acidification under aerobic conditions). The levels of both COX I and actin transcripts increased significantly during aerobic development. COX I mRNA levels were tightly correlated with previous measures of COX catalytic activity, which suggests that COX synthesis could be regulated by message concentration during aerobic development. The ontogenetic increase for these mRNAs was blocked by anoxia and aerobic acidosis. Importantly, the levels of COX I and actin mRNA did not decline appreciably during the 6 h bouts of quiescence, even though protein synthesis is acutely arrested by these same treatments. Thus, the constancy of mRNA levels during quiescence indicate that reduced protein synthesis is not caused by message limitation, but rather, is likely controlled at the translational level. One advantage of this regulatory mechanism is the conservation of mRNA molecules during quiescence, which would potentially favor a quick resumption of translation as soon as oxygen is returned to the embryos. Finally, because anoxia and aerobic acidosis are both characterized by acidic intracellular pH, the reduction in pH may serve, directly or indirectly, as one signal regulating levels of mRNA in this embryo during quiescence. PMID:8817476

  1. The Profile of Immune Modulation by Cannabidiol (CBD) Involves Deregulation of Nuclear Factor of Activated T Cells (NFAT)

    PubMed Central

    Kaplan, Barbara L. F.; Springs, Alison E. B.; Kaminski, Norbert E.

    2009-01-01

    Cannabidiol (CBD) is a cannabinoid compound derived from Cannabis Sativa that does not possess high affinity for either the CB1 or CB2 cannabinoid receptors. Similar to other cannabinoids, we demonstrated previously that CBD suppressed interleukin-2 (IL-2) production from phorbol ester plus calcium ionophore (PMA/Io)-activated murine splenocytes. Thus, the focus of the present studies was to further characterize the effect of CBD on immune function. CBD also suppressed IL-2 and interferon-γ (IFN-γ) mRNA expression, proliferation, and cell surface expression of the IL-2 receptor alpha chain, CD25. While all of these observations support the fact that CBD suppresses T cell function, we now demonstrate that CBD suppressed IL-2 and IFN-γ production in purified splenic T cells. CBD also suppressed activator protein-1 (AP-1) and nuclear factor of activated T cells (NFAT) transcriptional activity, which are critical regulators of IL-2 and IFN-γ. Furthermore, CBD suppressed the T cell-dependent anti-sheep red blood cell immunoglobulin M antibody forming cell (anti-sRBC IgM AFC) response. Finally, using splenocytes derived from CB1-/-/CB2-/- mice, it was determined that suppression of IL-2 and IFN-γ and suppression of the in vitro anti-sRBC IgM AFC response occurred independently of both CB1 and CB2. However, the magnitude of the immune response to sRBC was significantly depressed in CB1-/-/CB2-/- mice. Taken together, these data suggest that CBD suppresses T cell function and that CB1 and/or CB2 play a critical role in the magnitude of the in vitro anti-sRBC IgM AFC response. PMID:18656454

  2. Results of joint processing of data on nuclear and chemical explosions recorded on the long-range Quartz profile (Murmansk-Kyzyl)

    NASA Astrophysics Data System (ADS)

    Pavlenkova, G. A.; Pavlenkova, N. I.

    2008-04-01

    The long-range seismic profile Quartz, measured by the GEON Center (Ministry of Geology of the USSR), crosses a few large geostructures: the East European platform, Timan-Pechora plate, Northern Urals, West Siberian plate (WSP), and Altai. Observations of nuclear and chemical explosions were conducted on the profile. Joint processing of records from sources of both types provided detailed structures of the crust and upper mantle. They have confirmed the known patterns in the structure of these shells of the Earth and revealed new ones. Mountain roots are observed beneath the Urals and Altai, and areas of a higher heat flow are matched by lower velocity zones in the upper mantle. Moreover, it is shown that the Timan-Pechora plate is characterized by a two-layer crust untypical of other young plates of central Eurasia and the upper mantle has the same velocities beneath the ancient East European platform and the young Altai orogen. It is also shown that the vast region including the Timan-Pechora plate, Urals, and WSP is bounded on both sides by deep faults in the upper mantle dipping toward the center of Western Siberia. A few nearly continuous reflectors traceable in the upper mantle are represented by thin-layered heterogeneous beds. The largest horizontal heterogeneity is observed in the upper 100-km layer, often underlain by a lower velocity zone. The asthenosphere, as a layer of lower seismic velocities at the depth of a possible solidus (200 250 km), has not been revealed. The latter is evidently a feature specific to inner parts of the Eurasian continent; in marginal regions, e.g., in Western Europe, the asthenospheric layer is identified almost ubiquitously.

  3. BDE-47 causes developmental retardation with down-regulated expression profiles of ecdysteroid signaling pathway-involved nuclear receptor (NR) genes in the copepod Tigriopus japonicus.

    PubMed

    Hwang, Dae-Sik; Han, Jeonghoon; Won, Eun-Ji; Kim, Duck-Hyun; Jeong, Chang-Bum; Hwang, Un-Ki; Zhou, Bingsheng; Choe, Joonho; Lee, Jae-Seong

    2016-08-01

    2,2',4,4'-Tetrabromodiphenyl ether (BDE-47) is a persistent organic pollutant (POP) in marine environments. Despite its adverse effects (e.g. developmental retardation) in ecdysozoa, the effects of BDE-47 on transcription of ecdysteroid signaling pathway-involved-nuclear receptor (NR) genes and metamorphosis-related genes have not been examined in copepods. To examine the deleterious effect of BDE-47 on copepod molting and metamorphosis, BDE-47 was exposed to the harpacticoid copepod Tigriopus japonicus, followed by monitoring developmental retardation and transcriptional alteration of NR genes. The developmental rate was significantly inhibited (P<0.05) in response to BDE-47 and the agricultural insecticide gamma-hexachlorocyclohexane. Conversely, the ecdysteroid agonist ponasterone A (PoA) led to decreased molting and metamorphosis time (P<0.05) from the nauplius stage to the adult stage. In particular, expression profiles of all NR genes were the highest at naupliar stages 5-6 except for SVP, FTZ-F1, and HR96 genes. Nuclear receptor USP, HR96, and FTZ-F1 genes also showed significant sex differences (P<0.05) in gene expression levels over different developmental stages, indicating that these genes may be involved in vitellogenesis. NR gene expression patterns showed significant decreases (P<0.05) in response to BDE-47 exposure, implying that molting and metamorphosis retardation is likely associated with NR gene expression. In summary, BDE-47 leads to molting and metamorphosis retardation and suppresses transcription of NR genes. This information will be helpful in understanding the molting and metamorphosis delay mechanism in response to BDE-47 exposure. PMID:27337698

  4. To punish or to leave: distinct cognitive processes underlie partner control and partner choice behaviors.

    PubMed

    Martin, Justin W; Cushman, Fiery

    2015-01-01

    When a cooperative partner defects, at least two types of response are available: Punishment, aimed at modifying behavior, and ostracism, aimed at avoiding further social interaction with the partner. These options, termed partner control and partner choice, have been distinguished at behavioral and evolutionary levels. However, little work has compared their cognitive bases. Do these disparate behaviors depend on common processes of moral evaluation? Specifically, we assess whether they show identical patterns of dependence on two key dimensions of moral evaluation: A person's intentions, and the outcomes that they cause. We address this issue in a "trembling hand" economic game. In this game, an allocator divides a monetary stake between themselves and a responder based on a stochastic mechanism. This allows for dissociations between the allocator's intent and the actual outcome. Responders were either given the opportunity to punish or reward the allocator (partner control) or to switch to a different partner for a subsequent round of play (partner choice). Our results suggest that partner control and partner choice behaviors are supported by distinct underlying cognitive processes: Partner control exhibits greater sensitivity to the outcomes a partner causes, while partner choice is influenced almost exclusively by a partner's intentions. This cognitive dissociation can be understood in light of the unique adaptive functions of partner control and partner choice. PMID:25915550

  5. Depth profiles of radioactive cesium in soil using a scraper plate over a wide area surrounding the Fukushima Dai-ichi Nuclear Power Plant, Japan.

    PubMed

    Matsuda, Norihiro; Mikami, Satoshi; Shimoura, Susumu; Takahashi, Junko; Nakano, Masakazu; Shimada, Kiyotaka; Uno, Kiichiro; Hagiwara, Shigetomo; Saito, Kimiaki

    2015-01-01

    During the Fukushima Dai-ichi Nuclear Power Plant (NPP) accident, radioactive cesium was released in the environment and deposited on the soils. Depth profiles of radioactive cesium in contaminated soils provide useful information not only for radiation protection and decontamination operations but also for geoscience and radioecology studies. Soil samples were collected using a scraper plate three times between December 2011 and December 2012 at 84 or 85 locations within a 100-km radius of the Fukushima Dai-ichi NPP. In most of the obtained radioactive cesium depth profiles, it was possible to fit the concentration to a function of mass depth as either an exponential or hyperbolic secant function. By using those functions, following three parameters were estimated: (i) relaxation mass depth β (g cm(-2)), (ii) effective relaxation mass depth βeff (g cm(-2)), which is defined for a hyperbolic secant function as the relaxation mass depth of an equivalent exponential function giving the same air kerma rate at 1 m above the ground as the inventory, and (iii) 1/10 depth L1/10 (cm), at which the soil contains 90% of the inventory. The average β value (wet weight) including ones by hyperbolic secant function in December 2012, was 1.29 times higher than that in December 2011. In fact, it was observed that depth profiles at some study sites deviated from the typical exponential distributions over time. These results indicate the gradual downward migration of radioactive cesium in the soils. The L1/10 values in December 2012 were summarized and presented on a map surrounding the Fukushima Dai-ichi NPP, and the average value of L1/10 was 3.01 cm (n = 82) at this time. It was found that radioactive cesium remained within 5 cm of the ground surface at most study sites (71 sites). The sech function can also be used to estimate the downward migration rate V (kg m(-2) y(-1)). The V values in December 2012 (n = 25) were in good agreement with those found by a

  6. Computational protein design suggests that human PCNA-partner interactions are not optimized for affinity.

    PubMed

    Fridman, Yearit; Gur, Eyal; Fleishman, Sarel J; Aharoni, Amir

    2013-02-01

    Increasing the affinity of binding proteins is invaluable for basic and applied biological research. Currently, directed protein evolution experiments are the main approach for generating such proteins through the construction and screening of large mutant libraries. Proliferating cell nuclear antigen (PCNA) is an essential hub protein that interacts with many different partners to tightly regulate DNA replication and repair in all eukaryotes. Here, we used computational design to generate human PCNA mutants with enhanced affinity for several different partners. We identified double mutations in PCNA, outside the main partner binding site, that were predicted to increase PCNA-partner binding affinities compared to the wild-type protein by forming additional hydrophobic interactions with conserved residues in the PCNA partners. Affinity increases were experimentally validated with four different PCNA partners, demonstrating that computational design can reveal unexpected regions where affinity enhancements in natural systems are possible. The designed PCNA mutants can be used as a valuable tool for further examination of the regulation of PCNA-partner interactions during DNA replication and repair both in vitro and in vivo. More broadly, the ability to engineer affinity increases toward several PCNA partners suggests that interaction affinity is not an evolutionarily optimized trait of this system. PMID:23011891

  7. Slow Magic Angle Sample Spinning: A Non- or Minimally Invasive Method for High- Resolution 1H Nuclear Magnetic Resonance (NMR) Metabolic Profiling

    SciTech Connect

    Hu, Jian Z.

    2011-05-01

    High resolution 1H magic angle spinning nuclear magnetic resonance (NMR), using a sample spinning rate of several kHz or more (i.e., high resolution-magic angle spinning (hr-MAS)), is a well established method for metabolic profiling in intact tissues without the need for sample extraction. The only shortcoming with hr-MAS is that it is invasive and is thus unusable for non-destructive detections. Recently, a method called slow-MAS, using the concept of two dimensional NMR spectroscopy, has emerged as an alternative method for non- or minimal invasive metabolomics in intact tissues, including live animals, due to the slow or ultra-slow-sample spinning used. Although slow-MAS is a powerful method, its applications are hindered by experimental challenges. Correctly designing the experiment and choosing the appropriate slow-MAS method both require a fundamental understanding of the operation principles, in particular the details of line narrowing due to the presence of molecular diffusion. However, these fundamental principles have not yet been fully disclosed in previous publications. The goal of this chapter is to provide an in depth evaluation of the principles associated with slow-MAS techniques by emphasizing the challenges associated with a phantom sample consisting of glass beads and H2O, where an unusually large magnetic susceptibility field gradient is obtained.

  8. Collaborative vaccine development: partnering pays.

    PubMed

    Ramachandra, Rangappa

    2008-01-01

    Vaccine development, supported by infusions of public and private venture capital, is re-entering a golden age as one of the fastest growing sectors in the life-sciences industry. Demand is driven by great unmet need in underdeveloped countries, increased resistance to current treatments, bioterrorism, and for prevention indications in travelers, pediatric, and adult diseases. Production systems are becoming less reliant on processes such as egg-based manufacturing, while new processes can help to optimize vaccines. Expeditious development hinges on efficient study conduct, which is greatly enhanced through research partnerships with specialized contract research organizations (CROs) that are licensed and knowledgeable in the intricacies of immunology and with the technologic and scientific foundation to support changing timelines and strategies inherent to vaccine development. The CRO often brings a more objective assessment for probability of success and may offer alternative development pathways. Vaccine developers are afforded more flexibility and are free to focus on innovation and internal core competencies. Functions readily outsourced to a competent partner include animal model development, safety and efficacy studies, immunotoxicity and immunogenicity, dose response studies, and stability and potency testing. These functions capitalize on the CRO partner's regulatory and scientific talent and expertise, and reduce infrastructure expenses for the vaccine developer. Successful partnerships result in development efficiencies, elimination or reduced redundancies, and improved time to market. Keys to success include honest communications, transparency, and flexibility. PMID:18388488

  9. Semiconductor foundry, lithography, and partners

    NASA Astrophysics Data System (ADS)

    Lin, Burn J.

    2002-07-01

    The semiconductor foundry took off in 1990 with an annual capacity of less than 0.1M 8-inch-equivalent wafers at the 2-mm node. In 2000, the annual capacity rose to more than 10M. Initially, the technology practiced at foundries was 1 to 2 generations behind that at integrated device manufacturers (IDMs). Presently, the progress in 0.13-mm manufacturing goes hand-in-hand with any of the IDMs. There is a two-order of magnitude rise in output and the progress of technology development outpaces IDMs. What are the reasons of the success? Is it possible to sustain the pace? This paper shows the quick rise of foundries in capacity, sales, and market share. It discusses the their uniqueness which gives rise to advantages in conjunction with challenges. It also shows the role foundries take with their customer partners and supplier partners, their mutual dependencies, as well as expectations. What role then does lithography play in the foundries? What are the lithographic challenges to sustain the pace of technology? The experience of technology development and transfer, at one of the major foundries, is used to illustrate the difficulties and progresses made. Looking into the future, as semiconductor manufacturing will become even more expensive and capital investment more prohibitive, we will make an attempt to suggest possible solutions.

  10. To Punish or to Leave: Distinct Cognitive Processes Underlie Partner Control and Partner Choice Behaviors

    PubMed Central

    Martin, Justin W.; Cushman, Fiery

    2015-01-01

    When a cooperative partner defects, at least two types of response are available: Punishment, aimed at modifying behavior, and ostracism, aimed at avoiding further social interaction with the partner. These options, termed partner control and partner choice, have been distinguished at behavioral and evolutionary levels. However, little work has compared their cognitive bases. Do these disparate behaviors depend on common processes of moral evaluation? Specifically, we assess whether they show identical patterns of dependence on two key dimensions of moral evaluation: A person’s intentions, and the outcomes that they cause. We address this issue in a “trembling hand” economic game. In this game, an allocator divides a monetary stake between themselves and a responder based on a stochastic mechanism. This allows for dissociations between the allocator’s intent and the actual outcome. Responders were either given the opportunity to punish or reward the allocator (partner control) or to switch to a different partner for a subsequent round of play (partner choice). Our results suggest that partner control and partner choice behaviors are supported by distinct underlying cognitive processes: Partner control exhibits greater sensitivity to the outcomes a partner causes, while partner choice is influenced almost exclusively by a partner’s intentions. This cognitive dissociation can be understood in light of the unique adaptive functions of partner control and partner choice. PMID:25915550

  11. Intimate Partner Violence among California Couples: Multilevel Analysis of Environmental and Partner Risk Factors

    PubMed Central

    Cunradi, Carol B.; Todd, Michael; Mair, Christina; Remer, Lillian

    2014-01-01

    This study assessed the extent to which environmental (Census block-group alcohol outlet density, neighborhood demographic characteristics) and partner risk factors (e.g., hazardous drinking, psychosocial characteristics) contribute to the likelihood of intimate partner violence among 1,753 couples residing in 50 medium-to-large California cities. Multilevel logistic regression models were used to analyze the role of alcohol outlets (off-premise outlets, bars/pubs and restaurants), neighborhood demographic characteristics, and partner risk factors in relation to male-to-female partner violence (MFPV) and female-to-male partner violence (FMPV) risk. Approximately 12% of couples reported past-year partner violence. Results showed that none of the environmental measures were related to MFPV or FMPV. Male partner's impulsivity and each partner's adverse childhood experiences were associated with MFPV risk. Risk factors for FMPV were male partner's impulsivity and frequency of intoxication and female partner's adverse childhood experiences. Individual/couple characteristics appear to be the most salient IPV risk factors. The male partner's heavy drinking may lead to negative partner/spousal interactions that result in FMPV. The male partner's impulsivity, and each partner's adverse childhood experiences, may potentiate couple conflict and result in aggression. Interventions that target prevention of family dysfunction during childhood may help reduce interpersonal violence in adulthood. PMID:24812578

  12. Targeted disruption of the mouse Lipoma Preferred Partner gene

    SciTech Connect

    Vervenne, Hilke B.V.K.; Crombez, Koen R.M.O.; Delvaux, Els L.; Janssens, Veerle; Ven, Wim J.M. van de Petit, Marleen M.R.

    2009-02-06

    LPP (Lipoma Preferred Partner) is a zyxin-related cell adhesion protein that is involved in the regulation of cell migration. We generated mice with a targeted disruption of the Lpp gene and analysed the importance of Lpp for embryonic development and adult functions. Aberrant Mendelian inheritance in heterozygous crosses suggested partial embryonic lethality of Lpp{sup -/-} females. Fertility of Lpp{sup -/-} males was proven to be normal, however, females from Lpp{sup -/-} x Lpp{sup -/-} crosses produced a strongly reduced number of offspring, probably due to a combination of female embryonic lethality and aberrant pregnancies. Apart from these developmental and reproductive abnormalities, Lpp{sup -/-} mice that were born reached adulthood without displaying any additional macroscopic defects. On the other hand, Lpp{sup -/-} mouse embryonic fibroblasts exhibited reduced migration capacity, reduced viability, and reduced expression of some Lpp interaction partners. Finally, we discovered a short nuclear form of Lpp, expressed mainly in testis via an alternative promoter.

  13. Intimate partner violence in African American women.

    PubMed

    Campbell, Doris Williams; Sharps, Phyllis W; Gary, Faye A; Campbell, Jacquelyn C; Lopez, Loretta M

    2002-01-01

    Violence against African American women, specifically intimate partner abuse, has a significant impact on their health and well being. Intimate partner femicide and near fatal intimate partner femicide are the major causes of premature death and disabling injuries for African American women. Yet, despite this, there is a paucity of research and interventions specific and culturally relevant for these women. This article focuses on issues relevant to intimate partner violence and abuse against African American women by examining existing empirical studies of prevalence and health outcomes of intimate partner violence against women in general, plus what limited research there is about African American women, specifically. It includes a discussion of specific recommendations for research, practice, education, and policy to reduce and prevent intimate partner violence against African American women. PMID:12044219

  14. Putting intimate partner violence on your radar.

    PubMed

    Collett, DeShana; Bennett, Tamara

    2015-10-01

    Intimate partner violence is a preventable health problem that affects more than 12 million people in the United States each year. Those affected can be of any sex, race, ethnicity, socioeconomic status, religion, education level, or sexual orientation. All clinicians should screen for intimate partner violence as part of the routine history and physical examination. This article describes the dynamics of intimate partner violence and the 2013 screening guidelines from the US Preventive Services Task Force. PMID:26352870

  15. Health Problems of Partner Violence Victims

    PubMed Central

    Hines, Denise A.; Douglas, Emily M.

    2014-01-01

    Background National population-based studies show that 40%–50% of physical partner violence victims in a 1-year time period are men. However, studies assessing the health concerns related to partner violence victimization tend to focus on women, and none have assessed the health of male physical partner violence victims who sought help for their victimization. Purpose To understand men’s mental and physical health concerns that may be related to partner violence victimization. Methods In 2012–2013, two samples of men—611 physical partner violence victims who sought help and 1,601 men from a population-based sample – completed online questionnaires on their demographics, various types of partner violence victimization, physical health, mental health, and other risks. Data were analyzed using logistic regression, log binomial models, and robust Poisson models in 2013. Results In comparison to the population-based sample of men, male partner violence victims who sought help had significantly poorer health, particularly with regard to post-traumatic stress disorder, depression, high blood pressure, sexually transmitted diseases, and asthma. These differences remained after controlling for sample differences in demographics, substance use, previous traumatic exposure, and social support. Conclusions Practitioners should assess for health problems among partner violence victims and for partner violence victimization among men presenting with health problems. PMID:25442232

  16. Screening for Intimate Partner Violence.

    PubMed

    Paterno, Mary T; Draughon, Jessica E

    2016-05-01

    Intimate partner violence (IPV) is a serious concern for women that is associated with significant adverse health effects. Routine screening for IPV is recommended, but there are many barriers to screening that have been identified by providers, including discomfort, lack of training, and not knowing how to respond to a positive screen. This article reviews IPV screening and appropriate techniques for responding to a positive screen. IPV screening best practices include using a systematic protocol, developing a screening script, using a validated screening tool, and considerations for privacy and mandatory reporting. Responding to a positive screen should include acknowledging the experience, asking if the woman desires help, offering support and referrals, encouraging safety planning, and completing additional assessments to determine level of danger and to identify any comorbidities. Using these techniques along with therapeutic communication may increase IPV identification and create an environment in which women feel empowered to get help. PMID:26990666

  17. ECHO Status for International Partners

    NASA Technical Reports Server (NTRS)

    Weinstein, Beth; Lubelczyk, Jeff

    2006-01-01

    The EOS Clearinghouse (ECHO) is a clearinghouse of spatial and temporal metadata, inclusive of NASA's Distributed Active Archive Center (DAAC) data holdings, that enables the science community to more easily exchange NASA data and information. Currently, ECHO has metadata descriptors for over 55 million individual data granules and 13 million browse images. The majority of ECHO's holdings come directly from data held in the NASA DAACs. The science disciplines and domains represented in ECHO are diverse and include metadata for all of NASA's Science Focus Area data. As middleware for a service-oriented enterprise, ECHO offers access to its capabilities through a set of publicly available Application Program Interfaces (APIs). More information about ECHO is available at http://eos.nasa.gov.echo. The presentation will discuss the status of the ECHO Partners, holdings, and activities, including the transition from the EOS Data Gateway to the Warehouse Inventory Search Tool (WIST)

  18. Oligomerization and conformation change in solutions of calf lens gamma II-crystallin. Results from 1/T1 nuclear magnetic relaxation dispersion profiles.

    PubMed Central

    Koenig, S H; Beaulieu, C F; Brown, R D; Spiller, M

    1990-01-01

    From analyses of the magnetic field dependence of 1/T1 (nuclear magnetic relaxation dispersion [NMRD] profiles) of water protons in solutions of highly purified calf lens gamma II-crystallin, we find that monomers form oligomers at relatively low concentrations, which increase in size with increasing concentration and decreasing temperature. At approximately 16% by volume and -4 degrees C, the mean oligomeric molecular weight is approximately 120-fold greater than the monomeric value of 20 kD. Below this concentration, there is no indication of any substantive change in conformation of the monomeric subunits. At higher concentrations, the tertiary structure of the monomer appears to reconfigure rather abruptly, but reversibly, as evidenced by the appearance of spectra-like 14N peaks in the NMRD profiles. The magnitudes of these peaks, known to arise from cross-relaxation of water protons through access to amide (NH) moieties of the protein backbone, indicate that the high concentration conformation is not compact, but open and extended in a manner that allows enhanced interaction with solvent. The data are analogous to those found for homogenates of calf and chicken lens (Beaulieu, C. F., J. I. Clark, R. D. Brown III, M. Spiller, and S. H. Koenig. 1988. Magn. Reson. Med. 8:47-57; Beaulieu, C. F., R. D. Brown III, J. I. Clark, M. Spiller, and S. H. Koenig. 1989. Magn. Reson. Med. 10:62-72). This unusually large dependence of oligomeric size and conformation on concentration in the physiological range is suggested as the mechanism by which osmotic equilibrium is maintained, at minimal metabolic expense, in the presence of large gradients of protein concentration in the lens in vivo (cf Vérétout and Tardieu, 1989. Eur. Biophys. J. 17:61-68). Finally, the results of the NMRD data provide a ready explanation of the low temperature phase transition, and "cold-cataract" separation of phases, observed in gamma II-crystallin solutions; we suggest that the phases that

  19. Vertical distribution and temporal changes of 137Cs in soil profiles under various land uses after the Fukushima Dai-ichi Nuclear Power Plant accident.

    PubMed

    Takahashi, Junko; Tamura, Kenji; Suda, Tomoya; Matsumura, Ryo; Onda, Yuichi

    2015-01-01

    We monitored the vertical distribution of (137)Cs in soil profiles under eight different land uses for the 2 y after the Fukushima Dai-ichi Nuclear Power Plant accident, and discussed the temporal changes in the early-stage of the migration and the determinants of the initial distribution. The soil samples were collected for four surveys using a scraper plate at each study site, which consisted of three forests (mixed forest, mature cedar, and young cedar), two grasslands (pasture and meadow) and three abandoned agricultural fields (farm land, tobacco field, and paddy field). The land use patterns have a large influence on some soil properties and the migration processes of (137)Cs above ground, resulting in different distribution of (137)Cs in those soil profiles. Specifically, the secondary deposition of (137)Cs from the coniferous canopy, retention of (137)Cs by litter layer, and the homogenization of (137)Cs concentrations in surface soil by natural soil mixing such as the disturbance by cattle grazing, roots growing and the formation of needle ice were important to cause redistribution of the deposited (137)Cs. Only in the paddy field, the (137)Cs inventory in subsurface soils (5-10 cm) gradually increased and comprised 26% of the total (137)Cs in 2 y, showing the downward migration of (137)Cs to subsurface soil. In the other sites, it was considered that (137)Cs were strongly adsorbed by soil particles and rarely migrated downward as soluble form. Vertical distributions during the first survey were able to be used as the initial distributions and were well fitted to the exponential equation. The distribution parameters α (relaxation depth) and β (relaxation mass depth), calculated by the exponential equation were correlated with RIP (r = -0.806, p < 0.05), macro pore (r = 0.651, p = 0.11), and dispersible fine particle content (r = 0.856, p < 0.05). It indicated that the initial distribution would be influenced by the Cs fixation ability of

  20. Quantitative 1H Nuclear Magnetic Resonance Metabolite Profiling as a Functional Genomics Platform to Investigate Alkaloid Biosynthesis in Opium Poppy1[W

    PubMed Central

    Hagel, Jillian M.; Weljie, Aalim M.; Vogel, Hans J.; Facchini, Peter J.

    2008-01-01

    Opium poppy (Papaver somniferum) produces a diverse array of bioactive benzylisoquinoline alkaloids and has emerged as a versatile model system to study plant alkaloid metabolism. The plant is widely cultivated as the only commercial source of the narcotic analgesics morphine and codeine. Variations in plant secondary metabolism as a result of genetic diversity are often associated with perturbations in other metabolic pathways. As part of a functional genomics platform, we used 1H nuclear magnetic resonance (NMR) metabolite profiling for the analysis of primary and secondary metabolism in opium poppy. Aqueous and chloroform extracts of six different opium poppy cultivars were subjected to chemometric analysis. Principle component analysis of the 1H NMR spectra for latex extracts clearly distinguished two varieties, including a low-alkaloid variety and a high-thebaine, low-morphine cultivar. Distinction was also made between pharmaceutical-grade opium poppy cultivars and a condiment variety. Such phenotypic differences were not observed in root extracts. Loading plots confirmed that morphinan alkaloids contributed predominantly to the variance in latex extracts. Quantification of 34 root and 21 latex metabolites, performed using Chenomx NMR Suite version 4.6, showed major differences in the accumulation of specific alkaloids in the latex of the low-alkaloid and high-thebaine, low-morphine varieties. Relatively few differences were found in the levels of other metabolites, indicating that the variation was specific for alkaloid metabolism. Exceptions in the low-alkaloid cultivar included an increased accumulation of the alkaloid precursor tyramine and reduced levels of sucrose, some amino acids, and malate. Real-time polymerase chain reaction analysis of 42 genes involved in primary and secondary metabolism showed differential gene expression mainly associated with alkaloid biosynthesis. Reduced alkaloid levels in the condiment variety were associated with the

  1. Communication Partners' Journey through Their Partner's Hearing Impairment

    PubMed Central

    Manchaiah, Vinaya K. C.; Stephens, Dafydd; Lunner, Thomas

    2013-01-01

    The objective of this study was to further develop the Ida Institute model on communication partners' (CPs) journey through experiences of person with hearing impairment (PHI), based on the perspectives of CPs. Nine CPs of hearing aid users participated in this study, recruited through the Swansea hearing impaired support group. Semi-structured interviews were conducted, the data were analysed using qualitative thematic analysis and presented with the use of process mapping approach. Seven main phases were identified in the CP journey which includes: (1) contemplation, (2) awareness, (3) persuasion, (4) validation, (5) rehabilitation, (6) adaptation, and (7) resolution. The Ida Institute model (based on professionals' perspective) was compared with the new template developed (based on CPs' perspectives). The results suggest some commonalities and differences between the views of professionals and CPs. A new phase, adaptation, was identified from CPs reported experiences, which was not identified by professionals in the Ida Institute model. The CP's journey model could be a useful tool during audiological enablement/rehabilitation sessions to promote discussion between the PHI and the CP. In addition, it can be used in the training of hearing healthcare professionals. PMID:23533422

  2. Odd top partners at the LHC

    NASA Astrophysics Data System (ADS)

    Anandakrishnan, Archana; Collins, Jack H.; Farina, Marco; Kuflik, Eric; Perelstein, Maxim

    2016-04-01

    LHC searches for fermionic top partners T focus on three decay topologies: T →b W , T →t Z , and T →t h . However, top partners may carry new conserved quantum numbers that forbid these decays. The simplest possibility is a conserved parity, under which the top partner is odd and all SM states are even. In this case, decays of top partners may involve new particle-odd scalars, leading to signal topologies more commonly associated with supersymmetry, either with or without R -parity conservation. We study a simplified model in which this possibility is realized, and estimate the bounds on the top partner mass in this model implied by LHC searches for supersymmetry. We find that the bounds can be significantly weaker than in the conventional top partner decay scenario. For example, if the new parity is exact, a 500 GeV top partner is allowed as long as the lightest parity-odd scalar mass is between 325 and 500 GeV. The lower allowed top partner mass reduces the need for fine-tuning in the Higgs mass parameter, compared to the conventional decay scenario. We also present an explicit model, the oddest little Higgs, which exhibits this phenomenology.

  3. X (5568 ) and its partner states

    NASA Astrophysics Data System (ADS)

    Liu, Yan-Rui; Liu, Xiang; Zhu, Shi-Lin

    2016-04-01

    Stimulated by the recent observation of the X (5568 ), we study the X (5568 ) and its partners under the tetraquark scenario. In the framework of the color-magnetic interaction, we estimate the masses of the partner states of the X (5568 ) and discuss their decay pattern, which provides valuable information on the future experimental search of these states.

  4. 77 FR 76380 - Partner's Distributive Share

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-28

    ... Internal Revenue Service 26 CFR Part 1 RIN 1545-BJ37 Partner's Distributive Share AGENCY: Internal Revenue... attributes of its partners in order to determine whether a partnership's allocations are substantial. However..., provided the partnership's assumptions are reasonable, allocations that would be substantial on the...

  5. Intimate Partner Violence in Older Women

    ERIC Educational Resources Information Center

    Bonomi, Amy E.; Anderson, Melissa L.; Reid, Robert J.; Carrell, David; Fishman, Paul A.; Rivara, Frederick P.; Thompson, Robert S.

    2007-01-01

    Purpose: We describe the prevalence, types, duration, frequency, and severity of intimate partner violence ("partner violence") in older women. Design and Methods: We randomly sampled a total of 370 English-speaking women (65 years of age and older) from a health care system to participate in a cross-sectional telephone interview. Using 5…

  6. Involving Support Partners in Obesity Treatment

    ERIC Educational Resources Information Center

    Gorin, Amy; Phelan, Suzanne; Tate, Deborah; Sherwood, Nancy; Jeffery, Robert; Wing, Rena

    2005-01-01

    In this study, the authors examined whether the number or success of weight loss partners influences participants' outcomes in behavioral weight loss treatment. Overweight participants (n=109) assigned to an exercise intensive group in a larger trial were encouraged to invite up to 3 partners to attend treatment. Weight losses at 6, 12, and 18…

  7. Partners in Science. [CD-ROM].

    ERIC Educational Resources Information Center

    2000

    Partners in Science students, aided by community professionals, learn science by designing and conducting their own research. Partners in Science brings the community and schools closer together through a mentorship program. Scientists, in fields ranging from wildlife biology to space physics, are in frequent contact with classes and home-schooled…

  8. The Relationship Talk: Assessing Partner Commitment

    ERIC Educational Resources Information Center

    Nelms, Bobbie Jo; Knox, David; Easterling, Beth

    2012-01-01

    "The talk" is culturally understood to mean a discussion whereby both partners in a relationship reveal their feelings about each other and their commitment to a future together. Typically, one partner feels a greater need to clarity the future and instigates "the talk." This study reports the analysis of a 15 item questionnaire completed by 211…

  9. Advice from working women with retired partners.

    PubMed

    Cooley, Eileen L; Adorno, Gail

    2016-01-01

    in the 21st century, as more women are employed full-time and couples increasingly share egalitarian values, more women continue employment after their partners have voluntarily retired. However, we know very little about the experiences of this growing population of women. We asked working women with retired partners to share their advice for other women who may face this developmental transition. Open-ended responses from 97 women were analyzed to identify pertinent issues and themes. Four primary content areas were identified: time management, division of household labor, financial planning, and communication. Communication between partners was both a topic of concern as well as the solution suggested to resolve conflicts or differences that may arise when women live with a retired partner. It is expected that future changes in the workforce and improvements in the gender balance within relationships will continue to impact experiences for working women with retired partners. PMID:26933760

  10. Teleportation with Multiple Accelerated Partners

    NASA Astrophysics Data System (ADS)

    Sagheer, A.; Hamdoun, H.; Metwally, N.

    2015-09-01

    As the current revolution in communication is underway, quantum teleportation can increase the level of security in quantum communication applications. In this paper, we present a quantum teleportation procedure that capable to teleport either accelerated or non-accelerated information through different quantum channels. These quantum channels are based on accelerated multi-qubit states, where each qubit of each of these channels represents a partner. Namely, these states are the W state, Greenberger-Horne-Zeilinger (GHZ) state, and the GHZ-like state. Here, we show that the fidelity of teleporting accelerated information is higher than the fidelity of teleporting non-accelerated information, both through a quantum channel that is based on accelerated state. Also, the comparison among the performance of these three channels shows that the degree of fidelity depends on type of the used channel, type of the measurement, and value of the acceleration. The result of comparison concludes that teleporting information through channel that is based on the GHZ state is more robust than teleporting information through channels that are based on the other two states. For future work, the proposed procedure can be generalized later to achieve communication through a wider quantum network.

  11. Intimate Partner Violence and Partner STI Notification Among Adolescent and Young Adult Family Planning Clinic Patients

    PubMed Central

    Decker, Michele R.; Miller, Elizabeth; McCauley, Heather L.; Tancredi, Daniel J.; Levenson, Rebecca R.; Waldman, Jeffrey; Schoenwald, Phyllis; Silverman, Jay G.

    2013-01-01

    Objectives Patient-initiated partner STI notification, i.e., patients informing their sexual partners of diagnosis, is a cornerstone of STI prevention. Growing evidence suggests that women exposed to intimate partner violence (IPV) may fear such notification, or face negative consequences in response to STI disclosure. The current study assessed associations of IPV with fear of partner STI notification, and experiences of partner STI notification, among adolescent and young adult female family planning clinic patients. Methods Females patients ages 16–29 years in five family planning clinics in Northern California (n=1282) participated in a cross-sectional survey. Results History of physical or sexual IPV was associated with fear of partner STI notification. Moreover, participants exposed to IPV were more likely to have partners say it was not from them or otherwise accuse them of cheating in response to STI notification. Such partners were less likely to seek indicated STI treatment or testing. Conclusions Current findings suggest that STI partner notification may be compromised by IPV. Clinical practices and policies to support effective partner STI notification should include IPV assessment, and provide mechanisms to address related fears concerning partner notification. PMID:21680673

  12. Male partner selectivity, romantic confidence, and media depictions of partner scarcity.

    PubMed

    Taylor, Laramie D

    2013-01-01

    An experiment was conducted to explore the effects of exposure to partner scarcity or abundance messages on men's partner selectivity, romantic confidence, and self-assessed attractiveness. Undergraduate male participants watched a soap opera narrative featuring either two men competing over one potential female partner (partner scarcity) or two women competing over one potential male partner (partner abundance). Relative to control subjects, watching either narrative reduced romantic confidence. Experimental condition also affected partner selectivity and self-assessed attractiveness, though both effects were moderated by endorsement of traditional masculine ideology. Viewing the abundance narrative resulted in greater selectivity and self-assessed attractiveness for men high in endorsement of traditional masculinity but diminished selectivity and self-assessed attractiveness for men low in endorsement of traditional masculine identity. PMID:23335697

  13. Attitude and behavior changes among gay and bisexual men after use of rapid home HIV tests to screen sexual partners.

    PubMed

    Frasca, Timothy; Balan, Ivan; Ibitoye, Mobolaji; Valladares, Juan; Dolezal, Curtis; Carballo-Diéguez, Alex

    2014-05-01

    HIV testing can now be self-administered outside clinical settings through the purchase of home testing (HT) kits. Individuals also can use the kits to perform a test on a potential sexual partner prior to intercourse. We provided a 3-month supply of HT kits to men who reported multiple male partners and little or no condom use for anal intercourse. Participants used the test kits with partners in over 100 occasions. At the end of the study, approximately half of the participants described shifts in their attitudes and/or behaviors related to sexual risk. Reported changes included increased awareness of risk, increased discussion of STI/HIV safety measures, changes in partner choice and heightened consciousness of partner thinking. Easy access to HT kits may be a risk-reduction strategy for men with a high risk profile because their regular use could have an impact beyond the specific sexual encounter. PMID:24077975

  14. Sleep loss and partner violence victimization.

    PubMed

    Walker, Robert; Shannon, Lisa; Logan, T K

    2011-07-01

    Intimate partner violence victimization has been associated with serious health problems among women, including many disorders that involve sleep disturbances. However, there has been only limited examination of sleep duration among women with victimization experiences. A total of 756 women with a domestic violence order (DVO) against a male intimate partner were interviewed about their health, mental health, substance use, and partner violence victimization. Face-to-face interviews were conducted from February 2001 to November 2003 for data collection in three rural and one urban county representing different jurisdictional settings. Because the current analyses focused on understanding intimate partner victimization in the past year and associations with sleep disturbance, 147 participants were excluded for reporting a relationship with the DVO partner for less than 6 months in the past year. The final sample for this article was 609. The women reported an average of a little above 5.5 hours of sleep per night. For women in the current study, significant predictors of sleep disturbance included race, number of children, number of other symptoms of depression in the past 2 weeks excluding sleep criteria, number of other symptoms of PTSD in the past 2 weeks excluding sleep criteria, number of chronic physical health problems, and severity of physical violence by the DVO partner in the past year. Addressing short sleep duration among partner victims in health care settings might enhance safety planning and prevent the development of health/mental health problems that can arise from victimization. PMID:20587469

  15. Partners in Leadership for Pearl River

    NASA Technical Reports Server (NTRS)

    2007-01-01

    Members of the 2007 class of Partners in Leadership toured NASA Stennis Space Center in Hancock County, Miss., on Jan. 11. They visited the center's B Test Stand, part of the center's rocket engine test complex. The Partners in Leadership training program is designed to teach Pearl River County leaders about their county's government, economic development, health and human services, history and arts, environment and education during a 10-month period. The program, sponsored by the Partners for Pearl River County, helps fulfill the mission of the economic and community development agency.

  16. Intimate partner violence in rural environments.

    PubMed

    Annan, Sandra L

    2008-01-01

    The purpose of this chapter is to review nursing and other research related to rural intimate partner violence. The author presents a review of research in the area of intimate partner violence in the rural setting. The findings indicate that there is limited nursing research related to intimate partner violence in rural communities. The review describes the prevalence and types of abuse, the rural service issues, and the consequences of battering. The chapter also discusses the health implications of violence in the rural setting. The author concludes with a presentation of a research agenda for nursing research in rural environments. PMID:18709747

  17. Screening for Intimate Partner Violence During Pregnancy

    PubMed Central

    Deshpande, Neha A; Lewis-O’Connor, Annie

    2013-01-01

    Intimate partner violence (IPV) is defined as an actual or threatened abuse by an intimate partner that may be physical, sexual, psychological, or emotional in nature. Each year approximately 1.5 million women in the United States report some form of sexual or physical assault by an intimate partner; it is estimated that approximately 324,000 women are pregnant when violence occurs. Pregnancy may present a unique opportunity to identify and screen for patients experiencing IPV. This article provides health care practitioners and clinicians with the most current valid assessment and screening tools for evaluating pregnant women for IPV. PMID:24920977

  18. Cheating Partners, Conditional Probability and Contingency Tables

    ERIC Educational Resources Information Center

    Watson, Jane M.

    2011-01-01

    Developing statistical literacy for older school students includes recognizing and interpreting conditional statements in the media. Data on couples' success in predicting whether their partners have cheated provides a motivating context for teachers to lead their students through this process.

  19. Commercial Crew Program CCiCap Partners

    NASA Video Gallery

    NASA's Commercial Crew Program and its newest Commercial Crew Integrated Capability (CCiCap) partners are embracing the American spirit as they advance their integrated rocket and spacecraft design...

  20. Developing Community Partner Training: Regulations and Relationships

    PubMed Central

    Solomon, Stephanie; Piechowski, Patricia J.

    2012-01-01

    While funders increasingly support research that partners with communities, community partners still must submit to a regulatory oversight structure that does not reflect their unique research ethics challenges and needs. In recognition of the importance of collaborative research endeavors, the authors engaged in a process of reconnaissance and negotiations with local community partners and research ethics boards (REBs) at the University of Michigan to begin to address the mismatch between regulatory demands and community-based research realities. This preliminary process yielded both changes in the REB oversight structure and training required of community partnered research. While the ultimate impact of these changes remains to be seen, the process itself yielded insights and materials of use to both our local REBs, and hopefully those at other institutions as well. This article will present those insights and provide links to those materials. PMID:21680974

  1. Partnering with Families through Institutional Advancement.

    ERIC Educational Resources Information Center

    McInnis, Dion

    2001-01-01

    Because of limited resources and rising populations, more intentional relationships among university departments are needed to achieve common goals, especially in parent programming. Article focuses on novel partnering opportunities for student affairs and institutional advancement departments. (GCP)

  2. A framework for treating partner aggressive women.

    PubMed

    Dowd, Lynn; Leisring, Penny A

    2008-01-01

    Women are increasingly referred to intervention programs to address their use of physical violence against intimate partners. This article reviews the scant treatment outcome and attrition literature for partner aggressive women and describes important characteristics of partner aggressive women that must be taken into consideration in designing treatment. Recommended treatment modules are described in detail and include skill-building to enhance safety planning, conflict management, emotional regulation, communication and negotiation, and stress management. Additional modules should be included for some women based on individualized needs. These may include parenting skills and education and referral for treatment of conditions that undermine emotional stability, such as posttraumatic stress symptoms, substance abuse, and mood disorders. Treatment structure is outlined and pragmatic issues regarding the implementation of treatment are discussed. Interventions for partner aggressive woman must be designed to address women's victimization experiences as well as their perpetration. PMID:18624093

  3. Intimate Partner Violence among West African Immigrants

    PubMed Central

    AKINSULURE-SMITH, ADEYINKA M.; CHU, TRACY; KEATLEY, EVA; RASMUSSEN, ANDREW

    2013-01-01

    Although the number of African immigrants arriving to the United States has increased significantly, there has been little investigation regarding their experiences of intimate partner violence or coping strategies. This study used focus groups and individual interviews to explore intimate partner violence among 32 heterosexual West African immigrants. Results suggest that although cultural expectations influence their coping strategies, West African–born men and women face different realities, with women reporting multiple instances of abuse and a sense of frustration with the existing options for assistance. Although participants discussed multilevel support structures within the immediate West African community to address intimate partner violence, all of these options maintained a gender hierarchy, leaving women dissatisfied. Challenges and barriers to partner violence resolution and coping strategies are identified. Results are examined in terms of their implications for addressing the needs of this underserved population. Implications for future research and services are discussed and highlighted. PMID:23730146

  4. For Better Skin Cancer Checks, Partner Up

    MedlinePlus

    ... 159632.html For Better Skin Cancer Checks, Partner Up Melanoma survivors benefited when they and a loved ... the researchers explained. During two years of follow-up, 66 of the patients did go on to ...

  5. 76 FR 66012 - Partner's Distributive Share

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-10-25

    ...)(iii)(e) The de minimis partner rule in Sec. 1.704-1(b)(2)(iii)(e) (TD 9398, 73 FR 28699-01) was... Internal Revenue Service 26 CFR Part 1 RIN 1545-BJ37 Partner's Distributive Share AGENCY: Internal Revenue... through Friday between the hours of 8 a.m. and 4 p.m. to CC:PA:LPD:PR (REG- 109564-10), Courier's...

  6. Partner Choice in Raven (Corvus corax) Cooperation

    PubMed Central

    Asakawa-Haas, Kenji; Schiestl, Martina; Bugnyar, Thomas; Massen, Jorg J. M.

    2016-01-01

    Although social animals frequently make decisions about when or with whom to cooperate, little is known about the underlying mechanisms of partner choice. Most previous studies compared different dyads’ performances, though did not allow an actual choice among partners. We tested eleven ravens, Corvus corax, in triads, giving them first the choice to cooperate with either a highly familiar or a rather unfamiliar partner and, second, with either a friend or a non-friend using a cooperative string-pulling task. In either test, the ravens had a second choice and could cooperate with the other partner, given that this one had not pulled the string in the meantime. We show that during the experiments, these partner ravens indeed learn to wait and inhibit pulling, respectively. Moreover, the results of these two experiments show that ravens’ preferences for a specific cooperation partner are not based on familiarity. In contrast, the ravens did show a preference based on relationship quality, as they did choose to cooperate significantly more with friends than with non-friends and they were also more proficient when cooperating with a friend. In order to further identify the proximate mechanism of this preference, we designed an open-choice experiment for the whole group where all birds were free to cooperate on two separate apparatuses. This set-up allowed us to distinguish between preferences for close proximity and preferences to cooperate. The results revealed that friends preferred staying close to each other, but did not necessarily cooperate with one another, suggesting that tolerance of proximity and not relationship quality as a whole may be the driving force behind partner choice in raven cooperation. Consequently, we stress the importance of experiments that allow such titrations and, suggest that these results have important implications for the interpretations of cooperation studies that did not include open partner choice. PMID:27286247

  7. Perceptions of the physical attractiveness of the self, current romantic partners, and former partners.

    PubMed

    Swami, Viren; Allum, Lucy

    2012-02-01

    This study examined ratings of physical attractiveness of the self and former and current partners. A total of 304 participants completed measures of attractiveness, relationship satisfaction, love dimensions, self-esteem and sociosexual orientation. Consistent with previous work, results showed that participants rated their current partners as more attractive than themselves and their former partners. However, results also showed that former partners were rated as more attractive than the self on a number of bodily characteristics. Finally, results showed that ratings of former partner physical attractiveness were associated with passion for the former partner, self-esteem, sociosexual orientation, and attributions of relationship termination. These results are discussed in relation to the available literature on positive illusions in intimate relationships. PMID:21995611

  8. Partner choice creates fairness in humans.

    PubMed

    Debove, Stéphane; André, Jean-Baptiste; Baumard, Nicolas

    2015-06-01

    Many studies demonstrate that partner choice has played an important role in the evolution of human cooperation, but little work has tested its impact on the evolution of human fairness. In experiments involving divisions of money, people become either over-generous or over-selfish when they are in competition to be chosen as cooperative partners. Hence, it is difficult to see how partner choice could result in the evolution of fair, equal divisions. Here, we show that this puzzle can be solved if we consider the outside options on which partner choice operates. We conduct a behavioural experiment, run agent-based simulations and analyse a game-theoretic model to understand how outside options affect partner choice and fairness. All support the conclusion that partner choice leads to fairness only when individuals have equal outside options. We discuss how this condition has been met in our evolutionary history, and the implications of these findings for our understanding of other aspects of fairness less specific than preferences for equal divisions of resources. PMID:25972467

  9. Partner choice creates fairness in humans

    PubMed Central

    Debove, Stéphane; André, Jean-Baptiste; Baumard, Nicolas

    2015-01-01

    Many studies demonstrate that partner choice has played an important role in the evolution of human cooperation, but little work has tested its impact on the evolution of human fairness. In experiments involving divisions of money, people become either over-generous or over-selfish when they are in competition to be chosen as cooperative partners. Hence, it is difficult to see how partner choice could result in the evolution of fair, equal divisions. Here, we show that this puzzle can be solved if we consider the outside options on which partner choice operates. We conduct a behavioural experiment, run agent-based simulations and analyse a game-theoretic model to understand how outside options affect partner choice and fairness. All support the conclusion that partner choice leads to fairness only when individuals have equal outside options. We discuss how this condition has been met in our evolutionary history, and the implications of these findings for our understanding of other aspects of fairness less specific than preferences for equal divisions of resources. PMID:25972467

  10. Partner Caregiving in Older Cohabiting Couples

    PubMed Central

    2011-01-01

    Objectives. Despite the rapidly increasing prevalence of cohabitation among older adults, the caregiving literature has exclusively focused on formally married individuals. Extending prior work on intra-couple care, this study contrasts frail cohabitors’ patterns of care receipt from a partner to that of frail spouses. Methods. Using nationally representative panel data from the Health and Retirement Study (2000, 2002, 2004, and 2006), we estimate random effects cross-sectional times series models predicting frail cohabitors’ likelihood of receiving partner care compared with their married counterparts’. Conditional on the receipt of intra-couple care, we also examine differences in marital and nonmarital partners’ caregiving hours and caregiving involvement relative to other helpers. Results. Net of sociodemographic, disability, and comorbidity factors, we find that cohabitors are less likely to receive partner care than married individuals. However, caregiving nonmarital partners provide as many hours of care as spouses while providing a substantially larger share of disabled respondents’ care than marital partners. Discussion. Cohabitation and marriage have distinct implications for older adults’ patterns of partner care receipt. This study adds weight to a growing body of research emphasizing the importance of accounting for older adults’ nontraditional union forms and of examining the ramifications of cohabitation for older adults’ well-being. PMID:21482588

  11. 26 CFR 301.6224(c)-1 - Tax matters partner may bind nonnotice partners.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... of fraud, malfeasance, or misrepresentation of fact, if the tax matters partner enters into a... contained in 26 CFR part 1, revised April 1, 2001. ... 26 Internal Revenue 18 2010-04-01 2010-04-01 false Tax matters partner may bind nonnotice...

  12. 26 CFR 301.6224(c)-1 - Tax matters partner may bind nonnotice partners.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... of fraud, malfeasance, or misrepresentation of fact, if the tax matters partner enters into a... contained in 26 CFR part 1, revised April 1, 2001. ... 26 Internal Revenue 18 2011-04-01 2011-04-01 false Tax matters partner may bind nonnotice...

  13. 26 CFR 301.6224(c)-1 - Tax matters partner may bind nonnotice partners.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... of fraud, malfeasance, or misrepresentation of fact, if the tax matters partner enters into a... contained in 26 CFR part 1, revised April 1, 2001. ... 26 Internal Revenue 18 2012-04-01 2012-04-01 false Tax matters partner may bind nonnotice...

  14. 26 CFR 301.6224(c)-1 - Tax matters partner may bind nonnotice partners.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... of fraud, malfeasance, or misrepresentation of fact, if the tax matters partner enters into a... contained in 26 CFR part 1, revised April 1, 2001. ... 26 Internal Revenue 18 2014-04-01 2014-04-01 false Tax matters partner may bind nonnotice...

  15. 26 CFR 301.6224(c)-1 - Tax matters partner may bind nonnotice partners.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... of fraud, malfeasance, or misrepresentation of fact, if the tax matters partner enters into a... contained in 26 CFR part 1, revised April 1, 2001. ... 26 Internal Revenue 18 2013-04-01 2013-04-01 false Tax matters partner may bind nonnotice...

  16. A Community-Academic Partnered Grant Writing Series to Build Infrastructure for Partnered Research.

    PubMed

    King, Keyonna M; Pardo, Yvette-Janine; Norris, Keith C; Diaz-Romero, Maria; Morris, D'Ann; Vassar, Stefanie D; Brown, Arleen F

    2015-10-01

    Grant writing is an essential skill necessary to secure financial support for community programs and research projects. Increasingly, funding opportunities for translational biomedical research require studies to engage community partners, patients, or other stakeholders in the research process to address their concerns. However, there is little evidence on strategies to prepare teams of academic and community partners to collaborate on grants. This paper presents the description and formative evaluation of a two-part community-academic partnered grant writing series designed to help community organizations and academic institutions build infrastructure for collaborative research projects using a partnered approach. The first phase of the series was a half-day workshop on grant readiness, which was open to all interested community partners. The second phase, open only to community-academic teams that met eligibility criteria, was a 12-week session that covered partnered grant writing for foundation grants and National Institutes of Health grants. Participants in both phases reported an increase in knowledge and self-efficacy for writing partnered proposals. At 1-year follow-up, participants in Phase 2 had secured approximately $1.87 million in funding. This community-academic partnered grant writing series helped participants obtain proposal development skills and helped community-academic teams successfully compete for funding. PMID:26365589

  17. Examining intimate partner aggression assessment among returning veterans and their partners.

    PubMed

    LaMotte, Adam D; Taft, Casey T; Weatherill, Robin P; Scott, Jillian Panuzio; Eckhardt, Christopher I

    2014-03-01

    There is a growing research base focusing on intimate partner aggression (IPA) in combat veterans, although little work has focused on IPA assessment. In the current study, the authors investigated IPA assessment among 65 male Operation Iraqi Freedom (OIF) and Operation Enduring Freedom (OEF) combat veterans and their female partners. Specifically, we compared overall levels of veteran- and partner-perpetrated IPA, conducted concordance analyses to examine the degree of interpartner agreement on IPA occurrence and frequency, and investigated both veterans' and partners' relationship satisfaction and posttraumatic stress disorder (PTSD) symptoms as correlates of concordance. Results indicated that female partners perpetrated higher levels of physical IPA than did the male veterans, according to both veteran and combined reports. Concordance analyses revealed low to moderate levels of agreement between veterans and their partners on the perpetration of physical and psychological IPA, with particularly low agreement on the veterans' physical IPA. Female partners' relationship satisfaction was associated with reporting less of the veterans' and their own IPA relative to the veterans' reports, and their PTSD symptoms were associated with reporting more of the veterans' and their own IPA. In contrast, the veterans' PTSD symptoms were associated with reporting less of their own IPA relative to their partners' reports. The findings emphasize the need for those researching and treating IPA among military couples to assess IPA perpetrated by both members of the relationship and to consider possible factors that might impact the accuracy of IPA reporting. PMID:24079959

  18. Witness of Intimate Partner Violence in Childhood and Perpetration of Intimate Partner Violence in Adulthood

    PubMed Central

    Roberts, Andrea L.; Gilman, Stephen E.; Fitzmaurice, Garrett; Decker, Michele R.; Koenen, Karestan C.

    2011-01-01

    Background At least half a million women are victims of intimate partner violence in the United States annually, resulting in substantial harm. However, the etiology of violence to intimate partners is not well understood. Witnessing such violence in childhood has been proposed as a principal cause of adulthood perpetration, yet it remains unknown whether the association between witnessing intimate partner violence and adulthood perpetration is causal. Method We conducted a propensity-score analysis of intimate partner violence perpetration to determine whether childhood witnessing is associated with perpetration in adulthood, independent of a wide range of potential confounding variables, and therefore might be a causal factor. We used data from 14,564 U.S. men ages 20 and older from the 2004–2005 wave of the National Epidemiologic Survey on Alcohol and Related Conditions. Results Nearly 4% of men reported violent behavior toward an intimate partner in the past year. In unadjusted models, we found a strong association between childhood witnessing of intimate partner violence and adulthood perpetration (for witnessing any intimate partner violence, risk ratio [RR] = 2.6 [95% confidence interval = 2.1–3.2]; for witnessing frequent or serious violence, 3.0 [2.3–3.9]). In propensity-score models, the association was substantially attenuated (for witnessing any intimate partner violence, adjusted RR = 1.6 [1.2–2.0]; for witnessing frequent or serious violence, 1.6 [1.2–2.3]). Conclusions Men who witness intimate partner violence in childhood are more likely to commit such acts in adulthood, compared with men who are otherwise similar with respect to a large range of potential confounders. Etiological models of intimate partner violence perpetration should consider a constellation of childhood factors. PMID:20811285

  19. [Typology of incarcerated intimate partner aggressors].

    PubMed

    Loinaz, Ismael; Echeburúa, Enrique; Torrubia, Rafael

    2010-02-01

    Typology of incarcerated intimate partner aggressors. People who engage in intimate partner violence do not constitute a homogeneous group. Many studies in the Anglo-Saxon countries back the possibility of differentiating several subtypes of aggressors, but there are differences among them. One of the main applications of these typologies is the adaptation of the treatments to the subjects' characteristics. The aim of the present pilot study was to empirically establish a typology of batterers in Spain. The sample of 50 convicted violent intimate partner offenders was obtained from the Brians-2 penitentiary (Barcelona). Self-esteem, anger, cognitive distortions, and personality disorders were evaluated, as well as the frequency and type of violence. The results suggest the existence of two subtypes, distinguishable on the basis of the predictive dimensions, and so, partially confirm the typological proposals. PMID:20100435

  20. AIDS, partner notification and gender issues.

    PubMed

    Hildesheimer, Galya M

    2002-01-01

    The interplay between public health, human rights, legal obligations and physicians' ethical concerns was revealed dramatically in Israel in recent cases involving reporting of the HIV positive status of patients. This paper reviews and critically analyses the decision-making process of Israeli Ethics Committees regarding HIV partner notification. One aim of this analysis is to suggest principles that may guide the decision in such cases. It is argued that the resolution concerning disclosure to partners be based on the Principle of Proportionality, taking into account the type of relationship between partner and patient, their cultural background and gender issues. The paper demonstrates the advantages of addressing such issues by professional multidisciplinary Ethics Committees, whose decisions can bring to bear the scope of relevant considerations. PMID:12017441

  1. Parkinson patients as partners in care.

    PubMed

    Hirsch, Mark A; Sanjak, Mohammed; Englert, Danielle; Iyer, Sanjay; Quinlan, Margaret M

    2014-01-01

    Increasing physical activity, as part of an active lifestyle, is an important health goal for individuals with Parkinson's disease (PD). Exercise can positively impact health related quality of life. Given this, how can we promote physically active lifestyles among PD patients (most of whom are sedentary)? Here we suggest that health care professionals could significantly expand their impact by collaborating with PD patients and their spouses (or caregivers) as partners-in-care. We outline reasons why partners-in-care approaches are important in PD, including the need to increase social capital, which deals with issues of trust and the value of social networks in linking members of a community. We then present results of a qualitative study involving partners-in-care exercise beliefs among 19 PD patients and spouses, and conclude with our perspective on future benefits of this approach. PMID:24262175

  2. Sexuality and the Commission of Physical Violence to Partners and Non-Partners by Men and Women

    ERIC Educational Resources Information Center

    Cogan, Rosemary; Fennell, Tiffanie

    2007-01-01

    In 2 studies of physical violence and sexuality among college students, more than 75% of men and more than 60% of women reported committing physical violence in the past year, including more women to partners and more men to non-partners. More than 90% of men who committed violence to partners were also violent to non-partners. In Study 1, among…

  3. When a partner dies: lesbian widows.

    PubMed

    Bent, Katherine N; Magilvy, J Kathy

    2006-06-01

    Death of a life partner and the subsequent bereavement are profound experiences for an individual. By far, the majority of bereavement research reported is focused on heterosexual couples, primarily married and often in the later years of life. The purpose of this study was to describe the bereavement experiences of lesbians whose life partners have died. The study used a descriptive, qualitative design informed by feminist scholarship and phenomenology to access the depth of personal experiences, as well as internally interpreted meanings of those experiences, among lesbian widows. PMID:16613798

  4. Partners in policymaking: the first five years.

    PubMed

    Zirpoli, T J; Wieck, C; Hancox, D; Skarnulis, E R

    1994-12-01

    Many individuals with disabilities and their families are becoming empowered by learning effective self-advocacy strategies. In this article one enpowerment and self-advocacy training program, Partners in Policymaking, for parents of young children and adults with disabilities was described. Initially developed in Minnesota, the Partners program has completed its fifth year, has 163 graduates, and is being replicated in several other states. Follow-up data, qualitative and quantitative, were collected from program graduates. Results indicated both satisfaction with the program and the presence of many active citizen-advocates in the community. PMID:7854135

  5. Community-Partnered Research Conference Model: The Experience of Community Partners in Care Study

    PubMed Central

    Khodyakov, Dmitry; Pulido, Esmeralda; Ramos, Ana; Dixon, Elizabeth

    2013-01-01

    The Problem Conducting community-partnered research conferences is a powerful yet underutilized approach to translating research into practice and improving result dissemination and intervention sustainability strategies. Nonetheless, detailed descriptions of conference features and ways to use them in empirical research are rare. Purpose of Article We describe how community-partnered conferences may be integrated into research projects by using an example of Community Partners in Care, a large cluster-randomized controlled trial that uses Community Partnered Participatory Research principles. Key Points Our conceptual model illustrates the role community-partnered research conferences may play in three study phases and describes how different conference features may increase community engagement, build two-way capacity, and ensure equal project ownership. Conclusion(s) As the number of community-partnered studies grows, so too does the need for practical tools to support this work. Community-partnered research conferences may be effectively employed in translational research to increase two-way capacity-building and promote long-term intervention success. PMID:24859106

  6. Associations of unprotected anal intercourse with Grindr-met partners among Grindr-using young men who have sex with men in Los Angeles.

    PubMed

    Winetrobe, Hailey; Rice, Eric; Bauermeister, Jose; Petering, Robin; Holloway, Ian W

    2014-01-01

    Grindr, a geosocial smartphone application, is a networking medium for men who have sex with men. Although three quarters of young men who have sex with men (YMSM) Grindr users report having sex with a Grindr-met partner, the correlates of risky sexual behavior with Grindr-met partners are unknown. A randomly selected sample of 18- to 24-year-old, Grindr-using YMSM completed an anonymous online questionnaire assessing patterns of Grindr use and sexual behavior with their last Grindr-met partners. Of the 146 YMSM who reported having sex with Grindr-met partners, 20% had unprotected anal intercourse (UAI) at last sex with their Grindr-met partner. In the multivariable model, YMSM who used Grindr for at least one year showed naked chest/abs in their profile photo, and reported more past month Grindr-met partners were more likely to report UAI. These findings suggest that familiarity with the app was associated with YMSM's UAI with Grindr-met partners. Moreover, sexualized profile photos (i.e., naked chest/abs) may be associated with sexual risk-taking behaviors. HIV prevention interventions delivered or linked through such apps should target individuals who are longer/frequent users and who present sexualized profiles. PMID:24754563

  7. Toxicogenomic Dissection of the Perfluorooctanoic Acid Transcript Profile in Mouse Liver: Evidence for the Involvement of Nuclear Receptors PPARα and CAR

    EPA Science Inventory

    A number of perfluorinated alkyl acids including perfluorooctanoic acid (PFOA) elicit effects similar to peroxisome proliferator chemicals (PPC) in mouse and rat liver. There is strong evidence that PPC cause many of their effects linked to liver cancer through the nuclear recep...

  8. Individual differences in the propensity for partner sexual coercion.

    PubMed

    Camilleri, Joseph A; Quinsey, Vernon L

    2009-03-01

    Lalumière, Harris, Quinsey, and Rice (2005) proposed a three-path model (psychopathy, young male syndrome, and competitive disadvantage) of the development of sexually coercive behavior, but none of these individual difference characteristics have been tested among partner rapists. Using a community sample in Study 1, the authors find that psychopathy is the only significant predictor of self-reported propensity for partner sexual coercion. This model is tested in Study 2 by comparing convicted partner rapists, nonsexual partner assaulters, and heterosexual child molesters. One third of partner rapists are psychopaths, and their psychopathy scores are no different from those found in correctional samples. Partner rapists have an average IQ, providing further evidence that competitive disadvantage is less characteristic of partner rapists. There is some indication that partner rapists desist with age. The authors discuss these findings in light of recent findings that implicate cuckoldry risk in partner sexual assault. PMID:19218481

  9. Programs of 1993 Winning Teams: Pioneering Partners.

    ERIC Educational Resources Information Center

    1993

    Pioneering Partners for Educational Technology was created to enhance learning in K-12 classrooms by accelerating the use of educational technology. This document outlines the projects of the 1993 winning teams. The Illinois programs are: "A Travel Log Via Computer"; "Weatherization Audit Training for Teachers and Students"; and "Technology for…

  10. Head Injury in Partner-Abusive Men.

    ERIC Educational Resources Information Center

    Rosenbaum, Alan; And Others

    1994-01-01

    Fifty-three partner-abusive men, 45 maritally satisfied, and 32 maritally discordant, nonviolent men were evaluated for past history of head injury. Logistic regressions confirmed head injury was significant predictor of being a batterer. Implications of findings for both marital aggression and posthead injury rehabilitation are discussed. (Author)

  11. Gender Symmetry, Sexism, and Intimate Partner Violence

    ERIC Educational Resources Information Center

    Allen, Christopher T.; Swan, Suzanne C.; Raghavan, Chitra

    2009-01-01

    This study of a predominantly Hispanic sample of 92 male and 140 female college students examines both gender symmetry in intimate partner violence (IPV) and inconsistent relationships found in previous studies between sexist attitudes and IPV. Results indicate that although comparable numbers of men and women perpetrate and are victimized in…

  12. The Forgotten Partner: The Superintendent's Husband.

    ERIC Educational Resources Information Center

    Staples, Judith; Neal, Christine

    2000-01-01

    Female superintendents prize spouses who are emotionally secure and supportive and assist with household chores. Partners should communicate priorities, vacation together periodically, keep healthy, stay flexible, avoid guilt, establish family rituals, stay in frequent contact, respect each others' roles, and not strive for perfect balance. (MLH)

  13. Sustained Engagement with a Single Community Partner

    ERIC Educational Resources Information Center

    Lear, Darcy W.; Sanchez, Alejandro

    2013-01-01

    As scholarly work has recently turned its attention to the role of the community partner in Community Service-Learning (CSL) relationships, empirical frameworks for describing and executing community partnerships have emerged. This article applies those frameworks to one such partnership, which is presented from the perspective of both the…

  14. Intimate Partner Violence within Law Enforcement Families

    ERIC Educational Resources Information Center

    Anderson, Anita S.; Lo, Celia C.

    2011-01-01

    Using data from the Baltimore Police Stress and Domestic Violence study, the authors examined how exposure to stressful events on the job affects law enforcement employees' physical aggression toward domestic partners, evaluating the role of negative emotions and authoritarian spillover in mediating the impact of such task-related stress. The…

  15. Subtyping Male Perpetrators of Intimate Partner Violence

    ERIC Educational Resources Information Center

    Fowler, Katherine A.; Westen, Drew

    2011-01-01

    Domestic violence is a serious problem with far-reaching consequences. This study applies a new methodology to derive subtypes of male perpetrators of intimate partner violence. As part of a larger National Institute of Mental Health (NIMH)-funded study, a national sample of randomly selected psychologists and psychiatrists describe 188 adult male…

  16. Risk Recognition and Intimate Partner Violence

    ERIC Educational Resources Information Center

    Witte, Tricia H.; Kendra, Rachel

    2010-01-01

    The objective of this study was to determine whether female victims of physical forms of intimate partner violence (IPV) displayed deficits in risk recognition, or the ability to detect danger, in physically violent dating encounters. A total of 182 women watched a video depicting a psychologically and physically aggressive encounter between…

  17. Librarians as Community Partners: An Outreach Handbook

    ERIC Educational Resources Information Center

    Smallwood, Carol, Ed.

    2010-01-01

    Including 66 focused snapshots of outreach in action, this resource reflects the creative solutions of librarians searching for new and innovative ways to build programs that meet customer needs while expanding the library's scope into the community. This contributed volume includes: (1) A huge array of program options for partnering with other…

  18. Sleep Loss and Partner Violence Victimization

    ERIC Educational Resources Information Center

    Walker, Robert; Shannon, Lisa; Logan, T. K.

    2011-01-01

    Intimate partner violence victimization has been associated with serious health problems among women, including many disorders that involve sleep disturbances. However, there has been only limited examination of sleep duration among women with victimization experiences. A total of 756 women with a domestic violence order (DVO) against a male…

  19. Sound Partners. What Works Clearinghouse Intervention Report

    ERIC Educational Resources Information Center

    What Works Clearinghouse, 2010

    2010-01-01

    "Sound Partners" (Vadasy et al., 2004) is a phonics-based tutoring program that provides supplemental reading instruction to elementary school students grades K-3 with below average reading skills. The program is designed specifically for use by tutors with minimal training and experience. Instruction emphasizes letter-sound correspondences,…

  20. Expanding NASA Science Cooperation with New Partners

    NASA Astrophysics Data System (ADS)

    Allen, Marc; Bress, Kent

    Expanding NASA Science Cooperation with New Partners When NASA was created in 1958, it was given a goal of "cooperation by the United States with other nations and groups of nations in work done pursuant to this Act and in the peaceful application of the results." As science has become increasingly globalized during the past 50 years, NASA and its many partners in space and Earth science research have benefited enormously from pooling ideas, skills, and resources for joint undertakings. The discoveries made have powerfully advanced public awareness of science and its importance all over the world. Today, the U.S. Administra-tion is encouraging NASA to expand its cooperation with new and emerging partners. NASA space and Earth science cooperation is founded on scientist-to-scientist research collaboration. Space missions are very costly and technically challenging, but there are many other important areas for international cooperation. Areas ripe for expansion with new partners include space data sharing, scientist-to-scientist collaborative research, international research program plan-ning and coordination, Earth applications for societal benefit, ground-based measurements for Earth system science, and education and public outreach. This presentation lays out NASA's general principles for international science cooperation, briefly describes each of these opportu-nity areas, and suggests avenues for initiating new cooperative relationships.

  1. Parents-as-Partners-as-Learners Guide.

    ERIC Educational Resources Information Center

    Fear, Marion

    This guide provides information on the Parents-as-Partners-as-Learners project that is designed to encourage a love of reading in children and to increase communication between the home and school. Section A is an overview of the four phases of the project that are initiated by an adult literacy animator (facilitator) in a primary school: Phase I,…

  2. Women's Response to Intimate Partner Violence

    ERIC Educational Resources Information Center

    Ruiz-Perez, Isabel; Mata-Pariente, Nelva; Plazaola-Castano, Juncal

    2006-01-01

    The responses of women to a situation of abuse by their partner has hardly been addressed in the literature. Using a self-administered, anonymous questionnaire, 400 women attending three practices in a primary health care center in Granada (Spain) were studied. The women's response to abuse was used as a dependent variable. Sociodemographics,…

  3. Physical Health Effects of Intimate Partner Abuse

    ERIC Educational Resources Information Center

    Sillito, Carrie LeFevre

    2012-01-01

    Although intimate partner violence has been recognized as both a social problem and health issue, the extent to which it is a health issue for both males and females in the general population is largely unknown. This longitudinal research uses data from the National Survey of Family and Households (1987-2003). Random effects logistic regression…

  4. Strategy Guideline: Partnering for High Performance Homes

    SciTech Connect

    Prahl, D.

    2013-01-01

    High performance houses require a high degree of coordination and have significant interdependencies between various systems in order to perform properly, meet customer expectations, and minimize risks for the builder. Responsibility for the key performance attributes is shared across the project team and can be well coordinated through advanced partnering strategies. For high performance homes, traditional partnerships need to be matured to the next level and be expanded to all members of the project team including trades, suppliers, manufacturers, HERS raters, designers, architects, and building officials as appropriate. In an environment where the builder is the only source of communication between trades and consultants and where relationships are, in general, adversarial as opposed to cooperative, the chances of any one building system to fail are greater. Furthermore, it is much harder for the builder to identify and capitalize on synergistic opportunities. Partnering can help bridge the cross-functional aspects of the systems approach and achieve performance-based criteria. Critical success factors for partnering include support from top management, mutual trust, effective and open communication, effective coordination around common goals, team building, appropriate use of an outside facilitator, a partnering charter progress toward common goals, an effective problem-solving process, long-term commitment, continuous improvement, and a positive experience for all involved.

  5. Be a Partner in Clinical Research

    MedlinePlus

    ... part of the U.S. Department of Health and Human Services Latest Issue This Issue Features Be a Partner in Clinical Research Better Check Your Bowels Health Capsules Are You at Risk for Alcohol-Medication Interactions? Measles: Preventable with Vaccines Featured Website: National Center ...

  6. Programs of 1992 Winning Teams. Pioneering Partners.

    ERIC Educational Resources Information Center

    Council of Great Lakes Governors, Inc., Madison, WI.

    Pioneering Partners for Educational Technology was created to enhance learning in kindergarten through grade 12 by accelerating the use of educational technology. The program spotlights 24 project teams from Great Lakes states that are already using technology in creative ways in the following states: (1) Illinois; (2) Indiana; (3) Michigan; (4)…

  7. Gender, Psychopathy Factors and Intimate Partner Violence

    PubMed Central

    Mager, Kenna L.; Bresin, Konrad; Verona, Edelyn

    2014-01-01

    The present study sheds light on relationships between distinct psychopathic traits and perpetration of IPV in women versus men. Men and women with recent drug and/or violence histories (N = 250) were assessed for psychopathic traits using the Psychopathy Checklist: Screening Version and for their and their partner's use of IPV with the Revised Conflict Tactics Scale. The first goal was to examine the moderating role of gender in psychopathy factor relationships to IPV. Although both the interpersonal-affective traits (Factor1) and the impulsive-antisocial traits (Factor 2) of psychopathy were related to higher frequency of IPV perpetration, the relationship between Factor 1 and IPV was stronger in men. Our second goal examined the moderating role of psychopathy traits in the relationship between partner's perpetration of IPV and participant perpetration (mutual violence) in the two genders. Relationships between partner- and self-IPV were similar at both low and high levels of Factor 1 in men, although the partner- and self-IPV relationship was significantly stronger among women at low relative to high levels of Factor 1. The relationship between partner- and self-IPV was stronger at high levels of Factor 2 in men, whereas Factor 2 did not moderate mutual violence in women. These results indicate that relationships between psychopathy factors and IPV differ by gender, with psychopathy generally exacerbating IPV perpetration in men and Factor 1 traits playing a unique role in mutual violence in women. These findings add to the literature on female psychopathy and have important implications for future research on gender and IPV. PMID:25020252

  8. Exploring the intersection of partner stalking and sexual abuse.

    PubMed

    Logan, T K; Cole, Jennifer

    2011-07-01

    This study examined a range of sexually abusive acts women with protective orders against violent partners experienced using three groups: (a) women who never experience stalking or rape by the violent partner; (b) women who experienced stalking but who had never been raped by the violent partner; and (c) women who were stalked and raped by the violent partner. Findings suggest that women in violent relationships experienced a wide range of sexually abusive experiences and that there is a significant association of partner stalking and partner sexual abuse beyond rape. Implications for practice and future research are discussed. PMID:21665857

  9. Identification of FAM96B as a novel prelamin A binding partner.

    PubMed

    Xiong, Xing-Dong; Wang, Junwen; Zheng, Huiling; Jing, Xia; Liu, Zhenjie; Zhou, Zhongjun; Liu, Xinguang

    2013-10-11

    Prelamin A accumulation causes nuclear abnormalities, impairs nuclear functions, and eventually promotes cellular senescence. However, the underlying mechanism of how prelamin A promotes cellular senescence is still poorly understood. Here we carried out a yeast two-hybrid screen using a human skeletal muscle cDNA library to search for prelamin A binding partners, and identified FAM96B as a prelamin A binding partner. The interaction of FAM96B with prelamin A was confirmed by GST pull-down and co-immunoprecipitation experiments. Furthermore, co-localization experiments by fluorescent confocal microscopy revealed that FAM96B colocalized with prelamin A in HEK-293 cells. Taken together, our data demonstrated the physical interaction between FAM96B and prelamin A, which may provide some clues to the mechanisms of prelamin A in premature aging. PMID:24041693

  10. BWR AXIAL PROFILE

    SciTech Connect

    J. Huffer

    2004-09-28

    The purpose of this calculation is to develop axial profiles for estimating the axial variation in burnup of a boiling water reactor (BWR) assembly spent nuclear fuel (SNF) given the average burnup of an assembly. A discharged fuel assembly typically exhibits higher burnup in the center and lower burnup at the ends of the assembly. Criticality safety analyses taking credit for SNF burnup must account for axially varying burnup relative to calculations based on uniformly distributed assembly average burnup due to the under-burned tips. Thus, accounting for axially varying burnup in criticality analyses is also referred to as accounting for the ''end effect'' reactivity. The magnitude of the reactivity change due to ''end effect'' is dependent on the initial assembly enrichment, the assembly average burnup, and the particular axial profile characterizing the burnup distribution. The set of bounding axial profiles should incorporate multiple BWR core designs and provide statistical confidence (95 percent confidence that 95 percent of the population is bound by the profile) that end nodes are conservatively represented. The profiles should also conserve the overall burnup of the fuel assembly. More background on BWR axial profiles is provided in Attachment I.

  11. 76 FR 50715 - Briefing on Partner Vetting System Pilot Program

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-08-16

    ...; ] DEPARTMENT OF STATE Agency for International Development Briefing on Partner Vetting System Pilot Program... briefing. SUMMARY: Notice is hereby given of a briefing on the Partner Vetting System (PVS) pilot...

  12. Sex Partner with No Zika Symptoms Transmits Virus: CDC

    MedlinePlus

    ... html Sex Partner With No Zika Symptoms Transmits Virus: CDC New report also highlights ties between Zika ... News) -- U.S. health officials report that the Zika virus can be spread sexually even when a partner ...

  13. National Intimate Partner and Sexual Violence Survey: 2010 Highlights

    MedlinePlus

    National Intimate Partner and Sexual Violence Survey CDC’s National Intimate Partner and Sexual Violence Survey (NISVS) is an ongoing, nationally-representative telephone survey that collects detailed information ...

  14. HEIGHT, PARTNERS AND OFFSPRING: EVIDENCE FROM TAIWAN.

    PubMed

    Tao, Hung-Lin; Yin, Ching-Chen

    2016-09-01

    Using Taiwanese data, this study finds that tall males are more successful in mate selection and reproduction, but the results are weakly significant. Height is not helpful for females' reproductive success. Specifically, tall males are more likely to have a partner at present or in the past, have at least one child, have more children, have a shorter period of celibacy and have a longer time duration of living with a partner in their lifetime. Using mediation analysis, the study shows that tall males' reproductive success is not due to their achievements in the labour market (earnings), but is simply due to their height per se. Finally, a college student data set is used to explore the relation between height and dating hours. Tall male students have more dating hours, but no relation is found between females' height and dating hours. PMID:26449270

  15. Efficient DSMC collision-partner selection schemes.

    SciTech Connect

    Gallis, Michail A.; Torczynski, John Robert

    2010-07-01

    The effect of collision-partner selection schemes on the accuracy and the efficiency of the Direct Simulation Monte Carlo (DSMC) method of Bird is investigated. Several schemes to reduce the total discretization error as a function of the mean collision separation and the mean collision time are examined. These include the historically first sub-cell scheme, the more recent nearest-neighbor scheme, and various near-neighbor schemes, which are evaluated for their effect on the thermal conductivity for Fourier flow. Their convergence characteristics as a function of spatial and temporal discretization and the number of simulators per cell are compared to the convergence characteristics of the sophisticated and standard DSMC algorithms. Improved performance is obtained if the population from which possible collision partners are selected is an appropriate fraction of the population of the cell.

  16. Efficient DSMC collision-partner selection schemes.

    SciTech Connect

    Gallis, Michail A.; Torczynski, John Robert

    2010-05-01

    The effect of collision-partner selection schemes on the accuracy and the efficiency of the Direct Simulation Monte Carlo (DSMC) method of Bird is investigated. Several schemes to reduce the total discretization error as a function of the mean collision separation and the mean collision time are examined. These include the historically first sub-cell scheme, the more recent nearest-neighbor scheme, and various near-neighbor schemes, which are evaluated for their effect on the thermal conductivity for Fourier flow. Their convergence characteristics as a function of spatial and temporal discretization and the number of simulators per cell are compared to the convergence characteristics of the sophisticated and standard DSMC algorithms. Improved performance is obtained if the population from which possible collision partners are selected is an appropriate fraction of the population of the cell.

  17. Intimate partner violence and children's memory.

    PubMed

    Gustafsson, Hanna C; Coffman, Jennifer L; Harris, Latonya S; Langley, Hillary A; Ornstein, Peter A; Cox, Martha J

    2013-12-01

    The current study was designed to examine the relation between intimate partner violence (IPV) and children's memory and drew from a socioeconomically and racially diverse sample of children living in and around a midsized southeastern city (n = 140). Mother-reported IPV when the children were 30 months old was a significant predictor of children's short-term, working, and deliberate memory at 60 months of age, even after controlling for the children's sex and race, the families' income-to-needs ratio, the children's expressive vocabulary, and maternal harsh-intrusive parenting behaviors. These findings add to the limited extant literature that finds linkages between IPV and children's cognitive functioning and suggest that living in households in which physical violence is perpetrated among intimate partners may have a negative effect on multiple domains of children's memory development. PMID:24188084

  18. Nurses Christian Fellowship International: Partners in Care.

    PubMed

    White, Barbara

    2013-01-01

    The Nurses Christian Fellowship International Quadrennial Conference was held November 5-10, 2012, in Santiago, Chile. The theme, "Partners in Care: Unity in diversity through Christ" brought together nurses from all over the world for Bible teaching, education, networking, and fellowship. Plenary and session abstracts are available as supplemental digital content through the HTML and PDF versions of this article at journalofchristiannursing.com. PMID:23607156

  19. Social norms for intimate partner violence.

    PubMed

    Witte, Tricia H; Mulla, Mazheruddin M

    2013-01-01

    This study investigated perceived descriptive norms (i.e., perceived prevalence) for intimate partner violence (IPV) among college students. Male and female college students were asked to estimate the prevalence of IPV for same-sex "typical students" on their campus. Perpetrators of IPV made higher estimates than nonperpetrators. Both perpetrators and nonperpetrators overestimated the prevalence of IPV when compared to actual prevalence rates. Findings lend support for using social-norms-based prevention programs on college campuses. PMID:24547674

  20. Do aphids actively search for ant partners?

    PubMed

    Fischer, Christophe Y; Vanderplanck, Maryse; Lognay, Georges C; Detrain, Claire; Verheggen, François J

    2015-04-01

    The aphid-ant mutualistic relationships are not necessarily obligate for neither partners but evidence is that such interactions provide them strong advantages in terms of global fitness. While it is largely assumed that ants actively search for their mutualistic partners namely using volatile cues; whether winged aphids (i.e., aphids' most mobile form) are able to select ant-frequented areas had not been investigated so far. Ant-frequented sites would indeed offer several advantages for these aphids including a lower predation pressure through ant presence and enhanced chances of establishing mutuaslistic interactions with neighbor ant colonies. In the field, aphid colonies are often observed in higher densities around ant nests, which is probably linked to a better survival ensured by ants' services. Nevertheless, this could also result from a preferential establishment of winged aphids in ant-frequented areas. We tested this last hypothesis through different ethological assays and show that the facultative myrmecophilous black bean aphid, Aphis fabae L., does not orientate its search for a host plant preferentially toward ant-frequented plants. However, our results suggest that ants reduce the number of winged aphids leaving the newly colonized plant. Thus, ants involved in facultative myrmecophilous interactions with aphids appear to contribute to structure aphid populations in the field by ensuring a better establishment and survival of newly established colonies rather than by inducing a deliberate plant selection by aphid partners based on the proximity of ant colonies. PMID:24659520

  1. Restricting mutualistic partners to enforce trade reliance.

    PubMed

    Wyatt, Gregory A K; Kiers, E Toby; Gardner, Andy; West, Stuart A

    2016-01-01

    Mutualisms are cooperative interactions between members of different species, often involving the trade of resources. Here, we suggest that otherwise-cooperative mutualists might be able to gain a benefit from actively restricting their partners' ability to obtain resources directly, hampering the ability of the restricted partner to survive and/or reproduce without the help of the restricting mutualist. We show that (i) restriction can be favoured when it makes the resources of the restricting individual more valuable to their partner, and thus allows them to receive more favourable terms of trade; (ii) restriction maintains cooperation in conditions where cooperative behaviour would otherwise collapse; and (iii) restriction can lead to either an increase or decrease in a restricted individual's fitness. We discuss the applicability of this scenario to mutualisms such as those between plants and mycorrhizal fungi. These results identify a novel conflict in mutualisms as well as several public goods dilemmas, but also demonstrate how conflict can help maintain cooperation. PMID:26813888

  2. Restricting mutualistic partners to enforce trade reliance

    PubMed Central

    Wyatt, Gregory A. K.; Kiers, E. Toby; Gardner, Andy; West, Stuart A.

    2016-01-01

    Mutualisms are cooperative interactions between members of different species, often involving the trade of resources. Here, we suggest that otherwise-cooperative mutualists might be able to gain a benefit from actively restricting their partners' ability to obtain resources directly, hampering the ability of the restricted partner to survive and/or reproduce without the help of the restricting mutualist. We show that (i) restriction can be favoured when it makes the resources of the restricting individual more valuable to their partner, and thus allows them to receive more favourable terms of trade; (ii) restriction maintains cooperation in conditions where cooperative behaviour would otherwise collapse; and (iii) restriction can lead to either an increase or decrease in a restricted individual's fitness. We discuss the applicability of this scenario to mutualisms such as those between plants and mycorrhizal fungi. These results identify a novel conflict in mutualisms as well as several public goods dilemmas, but also demonstrate how conflict can help maintain cooperation. PMID:26813888

  3. Patterns in PARTNERing across Public Health Collaboratives

    PubMed Central

    Bevc, Christine A.; Retrum, Jessica H.; Varda, Danielle M.

    2015-01-01

    Inter-organizational networks represent one of the most promising practice-based approaches in public health as a way to attain resources, share knowledge, and, in turn, improve population health outcomes. However, the interdependencies and effectiveness related to the structure, management, and costs of these networks represents a critical item to be addressed. The objective of this research is to identify and determine the extent to which potential partnering patterns influence the structure of collaborative networks. This study examines data collected by PARTNER, specifically public health networks (n = 162), to better understand the structured relationships and interactions among public health organizations and their partners, in relation to collaborative activities. Combined with descriptive analysis, we focus on the composition of public health collaboratives in a series of Exponential Random Graph (ERG) models to examine the partnerships between different organization types to identify the attribute-based effects promoting the formation of network ties within and across collaboratives. We found high variation within and between these collaboratives including composition, diversity, and interactions. The findings of this research suggest common and frequent types of partnerships, as well as opportunities to develop new collaborations. The result of this analysis offer additional evidence to inform and strengthen public health practice partnerships. PMID:26445053

  4. Patterns in PARTNERing across Public Health Collaboratives.

    PubMed

    Bevc, Christine A; Retrum, Jessica H; Varda, Danielle M

    2015-10-01

    Inter-organizational networks represent one of the most promising practice-based approaches in public health as a way to attain resources, share knowledge, and, in turn, improve population health outcomes. However, the interdependencies and effectiveness related to the structure, management, and costs of these networks represents a critical item to be addressed. The objective of this research is to identify and determine the extent to which potential partnering patterns influence the structure of collaborative networks. This study examines data collected by PARTNER, specifically public health networks (n = 162), to better understand the structured relationships and interactions among public health organizations and their partners, in relation to collaborative activities. Combined with descriptive analysis, we focus on the composition of public health collaboratives in a series of Exponential Random Graph (ERG) models to examine the partnerships between different organization types to identify the attribute-based effects promoting the formation of network ties within and across collaboratives. We found high variation within and between these collaboratives including composition, diversity, and interactions. The findings of this research suggest common and frequent types of partnerships, as well as opportunities to develop new collaborations. The result of this analysis offer additional evidence to inform and strengthen public health practice partnerships. PMID:26445053

  5. Expression profiling of nuclear receptors in breast cancer identifies TLX as a mediator of growth and invasion in triple-negative breast cancer

    PubMed Central

    Remenyi, Judit; Banerji, Christopher R.S.; Lai, Chun-Fui; Periyasamy, Manikandan; Lombardo, Ylenia; Busonero, Claudia; Ottaviani, Silvia; Passey, Alun; Quinlan, Philip R.; Purdie, Colin A.; Jordan, Lee B.; Thompson, Alastair M.; Finn, Richard S.; Rueda, Oscar M.; Caldas, Carlos; Gil, Jesus; Coombes, R. Charles; Fuller-Pace, Frances V.; Teschendorff, Andrew E.; Buluwela, Laki; Ali, Simak

    2015-01-01

    The Nuclear Receptor (NR) superfamily of transcription factors comprises 48 members, several of which have been implicated in breast cancer. Most important is estrogen receptor-α (ERα), which is a key therapeutic target. ERα action is facilitated by co-operativity with other NR and there is evidence that ERα function may be recapitulated by other NRs in ERα-negative breast cancer. In order to examine the inter-relationships between nuclear receptors, and to obtain evidence for previously unsuspected roles for any NRs, we undertook quantitative RT-PCR and bioinformatics analysis to examine their expression in breast cancer. While most NRs were expressed, bioinformatic analyses differentiated tumours into distinct prognostic groups that were validated by analyzing public microarray data sets. Although ERα and progesterone receptor were dominant in distinguishing prognostic groups, other NR strengthened these groups. Clustering analysis identified several family members with potential importance in breast cancer. Specifically, RORγ is identified as being co-expressed with ERα, whilst several NRs are preferentially expressed in ERα-negative disease, with TLX expression being prognostic in this subtype. Functional studies demonstrated the importance of TLX in regulating growth and invasion in ERα-negative breast cancer cells. PMID:26280373

  6. Trends in Intimate Partner Violence: 1980-2008

    ERIC Educational Resources Information Center

    Powers, Rachael A.; Kaukinen, Catherine Elizabeth

    2012-01-01

    Research on trends in partner violence has primarily relied on official measures of victimization focusing primarily on women's risk for intimate partner homicide. The current study uses 28 years of data from the National Crime Victimization Survey (NCVS) to examine the trends of intimate partner violence against female victims and identify…

  7. 45 CFR 162.915 - Trading partner agreements.

    Code of Federal Regulations, 2010 CFR

    2010-10-01

    ... 45 Public Welfare 1 2010-10-01 2010-10-01 false Trading partner agreements. 162.915 Section 162... REQUIREMENTS ADMINISTRATIVE REQUIREMENTS General Provisions for Transactions § 162.915 Trading partner agreements. A covered entity must not enter into a trading partner agreement that would do any of...

  8. 12 CFR 1710.18 - Change of audit partner.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 12 Banks and Banking 7 2011-01-01 2011-01-01 false Change of audit partner. 1710.18 Section 1710... audit partner. An Enterprise may not accept audit services from an external auditing firm if the lead or coordinating audit partner who has primary responsibility for the external audit of the Enterprise, or...

  9. 12 CFR 1710.18 - Change of audit partner.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 12 Banks and Banking 9 2012-01-01 2012-01-01 false Change of audit partner. 1710.18 Section 1710... audit partner. An Enterprise may not accept audit services from an external auditing firm if the lead or coordinating audit partner who has primary responsibility for the external audit of the Enterprise, or...

  10. 12 CFR 1710.18 - Change of audit partner.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 12 Banks and Banking 9 2013-01-01 2013-01-01 false Change of audit partner. 1710.18 Section 1710... audit partner. An Enterprise may not accept audit services from an external auditing firm if the lead or coordinating audit partner who has primary responsibility for the external audit of the Enterprise, or...