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Sample records for poluprovodnikov tipa aiiibv

  1. Phase diagram calculation of AIIIBV binary solutions of the eutectic type in the generalized lattice model

    NASA Astrophysics Data System (ADS)

    Panov, G. A.; Zakharov, M. A.

    2015-11-01

    The present work is devoted to the phase diagrams calculation of AIIIBV systems within the framework of the generalized lattice model taking account of volume effects. The theoretically calculated phase diagram is compared with the corresponding experimental diagrams.

  2. Identification of cellular targets of a series of boron heterocycles using TIPA II-A sensitive target identification platform.

    PubMed

    Ward, Matthew S; Silva, Isba; Martinez, Walfre; Jefferson, Jameka; Rahman, Shakila; Garcia, Jeanie M; Kanichar, Divya; Roppiyakuda, Lance; Kosmowska, Ewa; Faust, Michelle A; Tran, Kim P; Chow, Felicia; Buglo, Elena; Zhou, Feimeng; Groziak, Michael P; Xu, H Howard

    2016-08-01

    One of the hurdles in the discovery of antibiotics is the difficulty of linking antibacterial compounds to their cellular targets. Our laboratory has employed a genome-wide approach of over-expressing essential genes in order to identify cellular targets of antibacterial inhibitors. Our objective in this project was to develop and validate a more sensitive disk diffusion based platform of target identification (Target Identification Platform for Antibacterials version 2; TIPA II) using a collection of cell clones in an Escherichia coli mutant (AS19) host with increased outer membrane permeability. Five known antibiotics/inhibitors and 28 boron heterocycles were tested by TIPA II assay, in conjunction with the original assay TIPA. The TIPA II was more sensitive than TIPA because eight boron heterocycles previously found to be inactive to AG1 cells in TIPA assays exhibited activity to AS19 cells. For 15 boron heterocycles, resistant colonies were observed within the zones of inhibition only on the inducing plates in TIPA II assays. DNA sequencing confirmed that resistant clones harbor plasmids with fabI gene as insert, indicating that these boron heterocycles all target enoyl ACP reductase. Additionally, cell-based assays and dose response curved obtained indicated that for two boron heterocycle inhibitors, the fabI cell clone in AG1 (wild-type) host cells exhibited at least 11 fold more resistance under induced conditions than under non-induced conditions. Moreover, TIPA II also identified cellular targets of known antibacterial inhibitors triclosan, phosphomycin, trimethoprim, diazaborine and thiolactomycin, further validating the utility of the new system. PMID:27301675

  3. The effect of temperature instability on the threshold sensitivity of photodetectors based on AIII-BV photodiodes

    NASA Astrophysics Data System (ADS)

    Aleksandrov, S. E.; Gavrilov, G. A.; Kapralov, A. A.; Sotnikova, G. Yu.

    2016-03-01

    The dependence of the sensitivity of photodetectors based on AIII-BV photodiodes on accidental variations of the temperature of its elements is analyzed. It is shown that the temperature drift of the bias level in input circuits of op-amps strongly contributes to the resulting photodetector noise up to frequencies on the order of 1 MHz. To reach the limiting sensitivities of the sensors, it is necessary to stabilize the temperature of not only the photodiode chip, but also the integrated circuit of the first amplifier stage. For most of applications, the required stabilization accuracy does not exceed ±0.1°C. As a result of the analysis, prototype high-sensitivity medium-wavelength (2-5 μm) sensors were developed that operate without forced cooling and have a detection threshold of tens of nanowatts at a detection bandwidth of 0-1 MHz.

  4. A disk-diffusion-based target identification platform for antibacterials (TIPA): an inducible assay for profiling MOAs of antibacterial compounds.

    PubMed

    Silva, Isba; Real, Lilian J; Ward, Matthew S; Xu, H Howard

    2014-06-01

    One of the challenges in antibiotic lead discovery is the difficulty and time-consuming task of determining the mechanism of action (MOA) of antibacterial compounds. In this report, we describe the development and validation of a facile and inexpensive assay system utilizing disk diffusion of inhibitors on solid agar medium embedded with mixed pools of a comprehensive collection of Escherichia coli clones each containing a plasmid-borne inducible essential gene from E. coli. From individual clones, pilot small-scale (48 or 50 clones) assays, to full-scale target identification platform for antibacterials (TIPA) system, involving a variety of assay formats (liquid vs solid media, individual vs mix clones), we demonstrate that elevated resistance phenotypes of relevant cell clones were highly specific. In particular, the TIPA system was able to reveal cellular targets of several known antibacterial inhibitors: cerulenin, diazaborine, indolmycin, phosphomycin, and triclosan. Complementary to several existing MOA profiling schemes, the TIPA system offers a simple and low-cost method for elucidating the target proteins of antibacterial inhibitors, thus will facilitate discovery and development of novel antibacterial compounds to combat multidrug-resistant bacterial pathogens. PMID:24622888

  5. Dislocation gliding and cross-hatch morphology formation in AIII-BV epitaxial heterostructures

    SciTech Connect

    Kovalskiy, V. A. Vergeles, P. S.; Eremenko, V. G.; Fokin, D. A.

    2014-12-08

    An approach for understanding the origin of cross-hatch pattern (CHP) on the surface of lattice mismatched GaMnAs/InGaAs samples grown on GaAs (001) substrates is developed. It is argued that the motion of threading dislocations in the (111) slip planes during the relaxation of InGaAs buffer layer is more complicated process and its features are similar to the ones of dislocation half-loops gliding in plastically deformed crystals. The heterostructures were characterized by atomic force microscopy and electron beam induced current (EBIC). Detailed EBIC experiments revealed contrast features, which cannot be accounted for by the electrical activity of misfit dislocations at the buffer/substrate interface. We attribute these features to specific extended defects (EDs) generated by moving threading dislocations in the partially relaxed InGaAs layers. We believe that the core topology, surface reconstruction, and elastic strains from these EDs accommodated in slip planes play an important role in the CHP formation. The study of such electrically active EDs will allow further understanding of degradation and changes in characteristics of quantum devices based on strained heterostructures.

  6. Dislocation gliding and cross-hatch morphology formation in AIII-BV epitaxial heterostructures

    NASA Astrophysics Data System (ADS)

    Kovalskiy, V. A.; Vergeles, P. S.; Eremenko, V. G.; Fokin, D. A.; Dorokhin, M. V.; Danilov, Yu. A.; Zvonkov, B. N.

    2014-12-01

    An approach for understanding the origin of cross-hatch pattern (CHP) on the surface of lattice mismatched GaMnAs/InGaAs samples grown on GaAs (001) substrates is developed. It is argued that the motion of threading dislocations in the {111} slip planes during the relaxation of InGaAs buffer layer is more complicated process and its features are similar to the ones of dislocation half-loops gliding in plastically deformed crystals. The heterostructures were characterized by atomic force microscopy and electron beam induced current (EBIC). Detailed EBIC experiments revealed contrast features, which cannot be accounted for by the electrical activity of misfit dislocations at the buffer/substrate interface. We attribute these features to specific extended defects (EDs) generated by moving threading dislocations in the partially relaxed InGaAs layers. We believe that the core topology, surface reconstruction, and elastic strains from these EDs accommodated in slip planes play an important role in the CHP formation. The study of such electrically active EDs will allow further understanding of degradation and changes in characteristics of quantum devices based on strained heterostructures.

  7. Effects of local field and inherent strain in reflectance anisotropy spectra of AIIIBV semiconductors with naturally oxidized surfaces

    NASA Astrophysics Data System (ADS)

    Berkovits, V. L.; Kosobukin, V. A.; Gordeeva, A. B.

    2015-12-01

    Reflectance anisotropy (RA) spectra of naturally oxidized (001) surfaces of GaAs and InAs crystals are measured for photon energies from 1.5 up to 5.5 eV. The differential high-accuracy RA spectra reveal features substantially different from those caused by either a reconstruction of clean surface or a built-in near-surface electric field. Models of atomic structure with anisotropic transition layers of excess arsenic atoms specific for GaAs(001)/oxide and InAs(001)/oxide interfaces are proposed. In conformity with these models, a general theory of reflectance anisotropy is developed for semiconductor/oxide interfaces within the Green's function technique. The theory takes into account the combined effect of local field due to interface dipoles and of intrinsic near-surface strain of the crystal. Measured RA spectra are analyzed in the model of valence-bond dipoles occupying a rectangular lattice in a multilayer medium. Comparing the measured and calculated spectra, we conclude that RA spectra of oxidized GaAs(001) and InAs(001) surfaces are simultaneously influenced by interface and near-surface anisotropies. The former is responsible for the broad-band spectral features which are associated with polarizability of the valence bonds attached to As atoms at the crystal/oxide interface. The near-surface anisotropy is due to inherent uniaxial straining the near-surface region of crystal. The effect of strain on RA spectra is experimentally and theoretically substantiated for GaAs crystal wafers undergone a uniaxial applied stress. Basically, this work results in the following. It establishes the physical nature of different levels of RA spectra observed in a majority of papers, but never analyzed. It demonstrates how the studied features of RA spectra could be applied for optical characterization of strained interfaces and atomic layers.

  8. Three coordination polymers based on different carboxylates, metals and a tri(4-imidazolylphenyl)amine ligand

    NASA Astrophysics Data System (ADS)

    Wu, Hua; Shi, Chenjie; Zhao, Yanqing; Jiang, Yutong; Tao, Yuehong

    2015-04-01

    In this paper, three new coordination complexes based on a flexible tri(4-imidazolylphenyl)amine (Tipa) ligand, namely [Co(Tipa)(L1)2]·H2O (1), [Zn2(Tipa)(L1)4(H2O)]·2H2O (2) and [Mn(Tipa)(L2)]·2H2O (3), where HL1 = benzoic acid H2L2 = 5-OH-1,3-benzenedicarboxylic acid and Tipa = tri(4-imidazolylphenyl)amine, have been synthesized under the hydrothermal condition and characterized by single-crystal X-ray diffraction, elemental analysis and IR spectra. Compound 1 exhibits a 1D ladder chain with the benzoic anions hanging on the two sides of the chain. For compound 2, it shows a fascinating 1D zigzag chain. Compound 3 displays (3,5)-connected (42·6)(42·67·8) topology, where the identical 2D networks entangle in highly rare parallel fashions to give a fascinating 2D → 3D framework with polycatenation and polyrotaxane characters. Moreover, the photoluminescent properties for the compounds 2 and 3 were also investigated.

  9. Syntheses, structures and tunable luminescence of lanthanide metal-organic frameworks based on azole-containing carboxylic acid ligand

    SciTech Connect

    Zhao, Dian; Rao, Xingtang; Yu, Jiancan; Cui, Yuanjing Yang, Yu; Qian, Guodong

    2015-10-15

    Design and synthesis of a series of isostructural lanthanide metal-organic frameworks (LnMOFs) serving as phosphors by coordinate the H{sub 2}TIPA (5-(1H-tetrazol-5-yl)isophthalic acid) ligands and lanthanide ions is reported. The color of the luminescence can be tuned by adjusting the relative concentration of the lanthanide ions in the host framework GdTIPA, and near-pure-white light emission can be achieved. - Graphical abstract: Lanthanide metal-organic frameworks (LnMOFs) with tunable luminescence were synthesized using an azole-containing carboxylic acid as ligand. - Highlights: • A series of isostructural LnMOFs serving as phosphor is reported. • We model the GdTIPA: Tb{sup 3+}, Eu{sup 3+} which can tune color and emit white light. • The scheme and mechanism of luminescent LnMOFs are also presented and discussed.

  10. TIP peptide inhalation in experimental acute lung injury: effect of repetitive dosage and different synthetic variants

    PubMed Central

    2014-01-01

    Background Inhalation of TIP peptides that mimic the lectin-like domain of TNF-α is a novel approach to attenuate pulmonary oedema on the threshold to clinical application. A placebo-controlled porcine model of acute respiratory distress syndrome (ARDS) demonstrated a reduced thermodilution-derived extravascular lung water index (EVLWI) and improved gas exchange through TIP peptide inhalation within three hours. Based on these findings, the present study compares a single versus a repetitive inhalation of a TIP peptide (TIP-A) and two alternate peptide versions (TIP-A, TIP-B). Methods Following animal care committee approval ARDS was induced by bronchoalveolar lavage followed by injurious ventilation in 21 anaesthetized pigs. A randomised-blinded three-group setting compared the single-dosed peptide variants TIP-A and TIP-B as well as single versus repetitive inhalation of TIP-A (n = 7 per group). Over two three-hour intervals parameters of gas exchange, transpulmonary thermodilution, calculated alveolar fluid clearance, and ventilation/perfusion-distribution were assessed. Post-mortem measurements included pulmonary wet/dry ratio and haemorrhage/congestion scoring. Results The repetitive TIP-A inhalation led to a significantly lower wet/dry ratio than a single dose and a small but significantly lower EVLWI. However, EVLWI changes over time and the derived alveolar fluid clearance did not differ significantly. The comparison of TIP-A and B showed no relevant differences. Gas exchange and ventilation/perfusion-distribution significantly improved in all groups without intergroup differences. No differences were found in haemorrhage/congestion scoring. Conclusions In comparison to a single application the repetitive inhalation of a TIP peptide in three-hour intervals may lead to a small additional reduction the lung water content. Two alternate TIP peptide versions showed interchangeable characteristics. PMID:24904234

  11. Structural basis and dynamics of multidrug recognition in a minimal bacterial multidrug resistance system

    PubMed Central

    Habazettl, Judith; Allan, Martin; Jensen, Pernille Rose; Sass, Hans-Jürgen; Thompson, Charles J.; Grzesiek, Stephan

    2014-01-01

    TipA is a transcriptional regulator found in diverse bacteria. It constitutes a minimal autoregulated multidrug resistance system against numerous thiopeptide antibiotics. Here we report the structures of its drug-binding domain TipAS in complexes with promothiocin A and nosiheptide, and a model of the thiostrepton complex. Drug binding induces a large transition from a partially unfolded to a globin-like structure. The structures rationalize the mechanism of promiscuous, yet specific, drug recognition: (i) a four-ring motif present in all known TipA-inducing antibiotics is recognized specifically by conserved TipAS amino acids; and (ii) the variable part of the antibiotic is accommodated within a flexible cleft that rigidifies upon drug binding. Remarkably, the identified four-ring motif is also the major interacting part of the antibiotic with the ribosome. Hence the TipA multidrug resistance mechanism is directed against the same chemical motif that inhibits protein synthesis. The observed identity of chemical motifs responsible for antibiotic function and resistance may be a general principle and could help to better define new leads for antibiotics. PMID:25489067

  12. Graphene-based and graphene-like materials

    NASA Astrophysics Data System (ADS)

    Ivanovskii, Alexander L.

    2012-07-01

    The review generalizes information on methods for modification of graphene-based carbon materials through creation of structural defects, introduction of substitutional impurities, adsorption of impurity atoms and mechanical deformations. Graphene ribbons and nano-flakes, graphene allotropes, as well as multi-layer graphenes and graphene-containing hybrid carbon structures are considered. Methods of synthesis and the results of studies on the properties and simulation of graphene derivatives (graphane, graphone and fluorographene) are discussed. The results obtained in studies of related non-carbon graphene-like materials including silicon-, germanium- and tin-based structural analogues of graphene, graphene-like binary phases SiC, GeC, SiGe, SiSn, etc., as well as compounds AIIIBV (white graphene, etc.) and AIIBVI (BeO, ZnO, ZnS) are generalized. Other graphene-like materials including metal dichalcogenides, oxides and carbides are briefly outlined. The bibliography includes 605 references.

  13. Properties and composition of anodic oxide layers of indium antimonide

    SciTech Connect

    Sorokin, I.N.; Gat'ko, L.E.; Nikitina, N.G.

    1985-09-01

    In recent years a number of optoelectronic devices based on narrowgap semiconductors of the AIIIBV type have been developed. One of the factors preventing widespread production of such devices is the inadequate study of the effect of the technology on the properties of insulator-semiconductor systems, of which anodic oxide films (AOF)--indium antimonide--are most promising. In this work the authors studied the dielectric properties and chemical composition of indium antimonide AOF as a function of their thicknesses and conditions of formation. It is determined that anodic indium antimonide oxide layers 90-110nm thick have high dielectric properties. It is also determined that an increase of the film thickness above 80100nm is accompanied by a decrease in the relative antimony content. The ratio of indium and antimony in oxide layers depends on the electrical conditions of oxidation of the semiconductor: the relative antimony content increases as a result of a decrease in the field intensity under conditions of constant voltage.

  14. Projects of High-Temperature Nuclear Reactors

    NASA Astrophysics Data System (ADS)

    Ekmanis, J.; Tomsons, E.; Zeltiņš, N.

    2013-04-01

    Part 2 of the overview gives emphasis to the projects of high-temperature NRs, whose development is an area of active engagement for the specialists from the USA, France, Japan, Russia, China, the Netherlands, and Germany. Projects of several powerful NRs of the HTGR type for commercial use had been worked out in the USA and Germany already by 1970 but not yet implemented. Augstas temperatūras ar gāzes dzesēšanu HTGR (High Temperature Gas cooled Reactor) tipa kodolreaktoru (KR) izstrādes koncepcija bija piedāvāta 1956. gadā Lielbritānijā. Apmēram tanī pašā laikā minētā tipa KR izstrādi uzsāka Vācijā un ASV. HTGR tipa KR kodoldegviela un kodoldegvielas atražošanas materiāla sīkās daļiņas ar diametru apmēram 0.5 mm pārklātas ar vairākām aizsargkārtām un atrodas grafīta neitronu palēninātājā, kas aizsargā daļiņas no neitronu palēninātāja un dzesētāja iedarbes. Augstas temperatūras KR bez hēlija gāzes siltumnesēja var izmantot šķidrus metālus (nātriju, svinu vai svina-bismuta sakausējumu) un izkausētu sāli. Pašlaik darbojās divi augstas temperatūras ar hēlija gāzi dzesēti eksperimentālie HTGR tipa KR. Viens Japānā "HTTR" no 1998. gada oktobra (sākts būvēt 1991. gada 15. martā) ar 30 MWth siltuma jaudu. Otrs Ķīnā "HTR-10" no 2000.gada decembra (sākts būvēt 1995. gada14. jūnijā) ar 10 MWth siltuma jaudu. Ķīnā Shandong provincē 2011.gada aprīlī uzsāka augstas temperatūras "HTR-PM" (High Temperature Gas-cooled Reactor - Pebble bed Module) tipa kodolreaktora celtniecību ar 250 MWth siltuma jaudu. Augstas temperatūras kodolreaktoru izstrādē pašlaik aktīvi iesaistīti ASV, Francijas, Japānas, Krievijas, Ķīnas, Nīderlandes un Vācijas speciālisti.

  15. High power GaSb PV cells with nanocrystalline Si inclusions in the space charge region

    NASA Astrophysics Data System (ADS)

    Kalinovsky, V. S.; Levin, R. V.; Pushniy, B. V.; Rumyntsev, V. D.; Andreev, V. M.

    2012-10-01

    In the work, a new model of connecting elements for monolithic multijunction solar cells (SCs) based on AIIIBV compounds has been proposed, where, instead of tunneling p++-n++ junction, connecting elements - layers with nanocrystalline inclusions in space charge region (SCR) have been elaborated. Such connecting elements exclude the effect of peak current and decrease the series resistance of the whole structure, which is particularly important in converting highly concentrated sunlight. Technological regimes for fabricating such junctions and results of their study are presented. The nanocrystalline silicon objects were formed in the SCR of GaSb p++-n++ and p-n junctions. To estimate the effect of the nanocrystalline Si objects on defect formation during the production of the photoactive GaSb p-n junctions, structures where the base n-layer of photoactive GaSb p-n junctions was connected to the GaSb substrate by the usual way, through a p++-n++ junction or by means of a layer with nanocrystalline Si objects were studied. Measurements for obtaining both dark and light I-V characteristics were carried out at room temperature within the current density range of 10-8-102A/cm2. Measurements for obtaining light load I-V characteristics were carried out at illumination by monochromatic radiation (λ = 1.3 μm, hv = 0.95 eV) up to 5 W/cm2 power. The investigations carried out have shown that introduction of nanocrystalline Si inclusions into the SCR of p-n junctions allows excluding the negative effect of peak current up to current densities of about 50 A/cm2 and decreasing the ohmic resistance of the serially connected p-n junctions down to about 0,01 Ohmṡcm2.

  16. Influence of ferrite phase in alite-calcium sulfoaluminate cements

    NASA Astrophysics Data System (ADS)

    Duvallet, Tristana Yvonne Francoise

    Since the energy crisis in 1970's, research on low energy cements with low CO2- emissions has been increasing. Numerous solutions have been investigated, and the goal of this original research is to create a viable hybrid cement with the components of both Ordinary Portland cement (OPC) and calcium sulfoaluminate cement (CSAC), by forming a material that contains both alite and calcium sulfoaluminate clinker phases. Furthermore, this research focuses on keeping the cost of this material reasonable by reducing aluminum requirements through its substitution with iron. The aim of this work would produce a cement that can use large amounts of red mud, which is a plentiful waste material, in place of bauxite known as an expensive raw material. Modified Bogue equations were established and tested to formulate this novel cement with different amounts of ferrite, from 5% to 45% by weight. This was followed by the production of cement from reagent chemicals, and from industrial by-products as feedstocks (fly ash, red mud and slag). Hydration processes, as well as the mechanical properties, of these clinker compositions were studied, along with the addition of gypsum and the impact of a ferric iron complexing additive triisopropanolamine (TIPA). To summarize this research, the influence of the addition of 5-45% by weight of ferrite phase, was examined with the goal of introducing as much red mud as possible in the process without negatively attenuate the cement properties. Based on this PhD dissertation, the production of high-iron alite-calcium sulfoaluminateferrite cements was proven possible from the two sources of raw materials. The hydration processes and the mechanical properties seemed negatively affected by the addition of ferrite, as this phase was not hydrated entirely, even after 6 months of curing. The usage of TIPA counteracted this decline in strength by improving the ferrite hydration and increasing the optimum amount of gypsum required in each composition

  17. Self-assembled copper(II) coordination polymers derived from aminopolyalcohols and benzenepolycarboxylates: structural and magnetic properties.

    PubMed

    Kirillov, Alexander M; Karabach, Yauhen Y; Haukka, Matti; Guedes da Silva, M Fatima C; Sanchiz, Joaquin; Kopylovich, Maximilian N; Pombeiro, Armando J L

    2008-01-01

    The new copper(II) or copper(II)/sodium(I) 1D coordination polymers [Cu2(Hmdea)2(mu-H2O)(mu2-tpa)]n.2nH2O (1), [Cu2(H2tipa)2(mu2-ipa)]n.4nH2O (2), [Cu2(H2tea)2Na(H2O)2(mu2-tma)]n.6nH2O (3), [Cu2(H2tea)2(mu2-ipa)]n.nH2O (4a), and [Cu2(H2tea)2{mu3-Na(H2O)3}(mu3-ipa)]n(NO3)n.0.5nH2O (4b) have been prepared in aqueous medium by self-assembly from copper(II) nitrate, aminopolyalcohols [methyldiethanolamine (H2mdea), triisopropanolamine (H3tipa), and triethanolamine (H3tea)] as main chelating ligands and benzenepolycarboxylic acids [terephthalic (H2tpa), isophthalic (H2ipa), and trimesic (H3tma) acid] as spacers. They have been characterized by IR spectroscopy, elemental and single-crystal X-ray diffraction analyses, the latter indicating the formation of unusual multinuclear metal cores interconnected by various benzenepolycarboxylate spacers, leading to distinct wavelike, zigzag, or linear 1D polymeric metal-organic chains. These are further extended to 2D or 3D hydrogen-bonded supramolecular networks via extensive interactions with the intercalated crystallization water molecules. The latter are associated, also with aqua ligands, by hydrogen bonds resulting in acyclic (H2O)3 clusters in 1, (H2O)8 clusters in 2, infinite 1D water chains in 3, and disordered water-nitrate associates in 4b, all playing a key role in the structure stabilization and its extension to further dimensions. Variable-temperature magnetic susceptibility measurements have shown that 1-4 exhibit a moderately strong ferromagnetic coupling through the alkoxo bridge. The small Cu-O-Cu bridging angle and the large out-of-plane displacement of the carbon atom of the alkoxo group accounts for this behavior. The magnetic data have been analyzed by means of a dinuclear and a 1D chain model, and the magnetic parameters have been determined. The magnetic exchange coupling in 3, to our knowledge, is the highest found in alkoxo-bridged copper(II) complexes. PMID:18069826

  18. Functional nanoparticles translocation into cell and adhesion force curve analysis.

    PubMed

    Lee, Haisung; Veerapandian, Murugan; Kim, Byung Tae; Yun, Kyusik; Seo, Soo-Won

    2012-10-01

    The aim of this research is to investigate the cell translocation of two functional nanoparticles (barium sulfate (BaSO4NPs), europium (III) doped gadolinium oxide nanoparticles (Gd2O3@EuNPs)) into A549 cells by Bio-Atomic Force Microscopy (Bio-AFM). Successful cell translocation of these two nanoparticles are ensured from the measurement of changes in the cell surface roughness and interaction (extension), retraction forces from the vertical deflection of tip towards substrate surfaces through force-distance curve slope analysis. Measurement of typical adhesion forces (i.e., extension and retraction) between the tip-substrate (0.0963 and 1.155 nN), tip-A549 cell substrate (0.1177 and 2.468 nN), tip-Gd2O3@EuNPs/A549 substrate (0.0785 and 0.4276 nN) and tip-BaSO4NPs/A549 substrate (0.518 and 6.838 nN) confirms the successful cell translocation of functional nanoparticles into A549 cells. Further the nanoscale resolution of topographical height and 3D images evinces the surface characteristics of normal A549 cells and nanoparticles translocated A549 cells. PMID:23421137

  19. Expression stability of 13 housekeeping genes during carbon starvation of Pseudomonas aeruginosa.

    PubMed

    Alqarni, Budoor; Colley, Brendan; Klebensberger, Janosch; McDougald, Diane; Rice, Scott A

    2016-08-01

    Quantitative real-time polymerase chain reaction (qRT-PCR) is a reliable technique for quantifying mRNA levels when normalised by a stable reference gene/s. Many putative reference genes are known to be affected by physiological stresses, such as nutrient limitation and hence may not be suitable for normalisation. In this study of Pseudomonas aeruginosa, the expression of 13 commonly used reference genes, rpoS, proC, recA, rpsL, rho, oprL, anr, tipA, nadB, fabD, ampC, algD and gyrA, were analysed for changes in expression under carbon starvation and nutrient replete conditions. The results showed that rpoS was the only stably expressed housekeeping gene during carbon starvation. In contrast, other commonly used housekeeping genes were shown to vary by as much as 10-100 fold under starvation conditions. This study has identified a suitable reference gene for qRT-PCR in P. aeruginosa during carbon starvation. The results presented here highlight the need to validate housekeeping genes under the chosen experimental conditions. PMID:27297333

  20. Reduction of Electric Breakdown Voltage in LC Switching Shutters / Elektriskās Caursites Sprieguma Samazināšana Šķidro Kristālu Šūnās

    NASA Astrophysics Data System (ADS)

    Mozolevskis, G.; Ozols, A.; Nitiss, E.; Linina, E.; Tokmakov, A.; Rutkis, M.

    2015-10-01

    Liquid crystal display (LCD) industry is among the most rapidly growing and innovating industries in the world. Here continuously much effort is devoted towards developing and implementing new types of LCDs for various applications. Some types of LCDs require relatively high voltages for their operation. For example, bistable displays, in which an altering field at different frequencies is used for switching from clear to scattering states and vice versa, require electric fields at around 10 V/μm for operation. When operated at such high voltages an electrical breakdown is very likely to occur in the liquid crystal (LC) cell. This has been one of the limiting factors for such displays to reach market. In the present paper, we will report on the results of electrical breakdown investigations in high-voltage LC cells. An electrical breakdown in the cell is observed when current in the liquid crystal layer is above a specific threshold value. The threshold current is determined by conductivity of the liquid crystal as well as point defects, such as dust particles in LC layer, pinholes in coatings and electrode hillocks. In order to reduce the currents flowing through the liquid crystal layer several approaches, such as electrode patterning and adding of various buffer layers in the series with LC layer, have been tested. We demonstrate that the breakdown voltages can be significantly improved by means of adding insulating thin films. Šķidro kristālu ekrānu (LCD) industrija ir viena no visstraujāk augošajām industrijām pasaulē. Daudz pūļu un resursu tiek veltīti jauna tipa LCD izstrādē dažādiem pielietojumiem. Atsevišķa tipa LCD funkcionēšanai nepieciešami augsti spriegumi. Piemēram, bistabilos LCD, kuros izkliedējošs (ieslēgts) un dzidrs (izslēgts) stāvoklis tiek iegūts ar dažādu frekvenču maiņsprieguma palīdzību, elektriskā lauka intensitāte šķidrā kristāla slānī var sasniegt pat 10 V/μm. Augstās elektriskā lauka intensit

  1. Investigation Into the Accuracy of 3D Surface Roughness Characteristics

    NASA Astrophysics Data System (ADS)

    Kumermanis, M.; Rudzitis, J.; Mozga, N.; Ancans, A.; Grislis, A.

    2014-04-01

    The existing standards for surface roughness cover only two dimensions, while in reality this is three-dimensional (3D). In particular, the 3D surface roughness parameters are important for solving the contact surface mechanics problems as related to the accuracy of 3D surface roughness characteristics. One of the most important factors for determination of 3D characteristics is the number of data points (NDP) on the x- and y-axes (i.e. in cut-off length). The NDP has a profound effect on the accuracy of measurement results, measuring time and volume of the output data (especially along the y-axis, where the NDP is identical to the number of parallel profiles). At a too small NDP the results will be incorrect and with too broad scatter, while a too large NDP - though not enlarging the range of basic information - considerably increases the measuring time. Therefore, the aim of the work was to find the optimal NDP for such surface processing methods as grinding, spark erosion and shot methods of surface treatment. Eksistējošie virsmas raupjuma standarti apskata virsmas raupjumu tikai divās dimensijās. Tomēr reālais virsmas raupjums pēc savas dabas ir trīsdimensiju (3D) objekts. Līdz ar to virsmas raupjums ir jāraksturo ar 3D parametriem. Un no šo parametru noteikšanas precizitātes ir atkarīgi tālākie virsmas aprēķini, piemēram, virsmu kontaktēšanās process. Viens no svarīgākajiem faktoriem, raksturojot virsmas raupjumu 3D, pielietojot kontakta tipa mēriekārtas, ir datu punktu skaits pa abām mērīšanas asīm x un y. Ar datu punktu skaitu mēs saprotam to skaitu mērīšanas bāzes garumā. Datu punktu skaits būtiski ietekmē sagaidāmo mērījumu rezultātu precizitāti, mērīšanai nepieciešamo laiku un izejas datu faila izmērus (sevišķi y-ass virzienā, kur katrs datu punkts ir paralēls profils). Datu punktu skaitam ir jābūt optimālam. Pārāk mazs punktu skaits noved pie neprecīziem rezultātiem un lielas to izkliedes, savuk

  2. Sail-Type Wind Turbine for Autonomous Power Supplay: Possible Use in Latvia

    NASA Astrophysics Data System (ADS)

    Sakipova, S.; Jakovics, A.

    2014-12-01

    Under the conditions of continuous increase in the energy consumption, sharply rising prices of basic energy products (gas, oil, coal), deterioration of environment, etc., it is of vital importance to develop methods and techniques for heat and power generation from renewables. The paper considers the possibility to use a sail-type wind turbine for autonomous power supply in Latvia, taking into account its climatic conditions. The authors discuss the problems of developing a turbine of the type that would operate efficiently at low winds, being primarily designed to supply power to small buildings and farms distant from centralized electricity networks. The authors consider aerodynamic characteristics of such a turbine and the dependence of the thrust moment of its pilot model on the airflow rate at different angles of attack. The pilot model with a changeable blade shape has been tested and shows a good performance. Darbs veltīts vienam no atjaunojamo enerģiju veidiem - vēja enerģijai, analizētas tās izmantošanas iespējas. Vispirms īsi raksturota vēja enerģijas izmantošana pasaulē, kā arī vērtētas izmantošanas perspektīvas no inženiertehniskā un klimatisko apstākļu viedokļa. Turpinājumā raksturota situācija Latvijā, t. sk., arī vēja potenciāla pieejamība dažādos reģionos, kā arī vēja enerģijas izmantošanas efektivitāti raksturojošie lielumi. Konstatējot problēmu, rast vēja turbīnu risinājumus Latvijas apstākļiem ar maziem vidējiem vēja ātrumiem, izveidots buras tipa turbīnas modelis. Šī modeļa raksturlielumu izpēte veikta vēja tunelī Karagandas universitātē, konstatējot relatīvi labus efektivitātes rādītājus tiešai un pretējai vēja plūsmai. Izmantojot mērījumu rezultātus, ierosināti sistēmas uzlabojumi, kurus plānots pētīt turpmāk. Šādas sistēmas potenciāli var rast lietojumu tur, kur nepieciešami autonomi enerģijas avoti.

  3. Power Generation Investment Planning in a Modern Power System With High Share of Renewables

    NASA Astrophysics Data System (ADS)

    Oleinikova, I.; Ruksans, O.; Turcik, M.

    2014-04-01

    enerācijas tehnoloģijas izmaksu novērtēšana; - elektrostaciju ģenerācijas īstermiņa un ilgtermiņa izmaksu novērtēšana; - elektrostaciju ģenerācijas investīciju un vadības izmaksu novērtēšana; - dažādu ģenerācijas tehnoloģiju izmaksu skaitliskie aprēķini un analīze. Īpaša uzmanība veltīta investīciju plānošanai elektroenerģijas ražošanas nozarē liberalizēta tirgus apstākļos; tika izanalizēti efektīvie instrumenti, izmaksu metodes un ekonomiskās efektivitātes novērtēšanas nozīme dažāda tipa ģenerējošām jaudām un tās atbilstība mūsdienu elektroapgādes sistēmas prasībām