Sample records for por moraxella catarrhalis

  1. Moraxella catarrhalis in Chronic Obstructive Pulmonary Disease

    PubMed Central

    Murphy, Timothy F.; Brauer, Aimee L.; Grant, Brydon J. B.; Sethi, Sanjay

    2005-01-01

    Rationale: Moraxella catarrhalis is frequently present in the sputum of adults with chronic obstructive pulmonary disease (COPD). Little is known about the role of M. catarrhalis in this common disease. Objective: To elucidate the burden of disease, the dynamics of carriage, and immune responses to M. catarrhalis in COPD. Methods: Prospective cohort study of 104 adults with COPD in an outpatient clinic at the Buffalo Veterans Affairs Medical Center. Measurements: Clinical information, sputum cultures, molecular typing of isolates, and immunoassays to measure antibodies to M. catarrhalis. Main Results: Over 81 months, 104 patients made 3,009 clinic visits, 560 during exacerbations. Molecular typing identified 120 episodes of acquisition and clearance of M. catarrhalis in 50 patients; 57 (47.5%) of the acquisitions were associated with clinical exacerbations. No instances of simultaneous acquisition of a new strain of another pathogen were observed. The duration of carriage of M. catarrhalis was shorter with exacerbations compared with asymptomatic colonization (median, 31.0 vs. 40.4 days; p = 0.01). Reacquisition of the same strain was rare. The intensity of the serum IgG response was greater after exacerbations than asymptomatic colonization (p = 0.009). Asymptomatic colonization was associated with a greater frequency of a sputum IgA response than exacerbation (p = 0.009). Conclusions: M. catarrhalis likely causes approximately 10% of exacerbations of COPD, accounting for approximately 2 to 4 million episodes annually. The organism is cleared efficiently after a short duration of carriage. Patients develop strain-specific protection after clearance of M. catarrhalis from the respiratory tract. PMID:15805178

  2. Moraxella catarrhalis synthesizes an autotransporter that is an acid phosphatase.

    PubMed

    Hoopman, Todd C; Wang, Wei; Brautigam, Chad A; Sedillo, Jennifer L; Reilly, Thomas J; Hansen, Eric J

    2008-02-01

    Moraxella catarrhalis O35E was shown to synthesize a 105-kDa protein that has similarity to both acid phosphatases and autotransporters. The N-terminal portion of the M. catarrhalis acid phosphatase A (MapA) was most similar (the BLAST probability score was 10(-10)) to bacterial class A nonspecific acid phosphatases. The central region of the MapA protein had similarity to passenger domains of other autotransporter proteins, whereas the C-terminal portion of MapA resembled the translocation domain of conventional autotransporters. Cloning and expression of the M. catarrhalis mapA gene in Escherichia coli confirmed the presence of acid phosphatase activity in the MapA protein. The MapA protein was shown to be localized to the outer membrane of M. catarrhalis and was not detected either in the soluble cytoplasmic fraction from disrupted M. catarrhalis cells or in the spent culture supernatant fluid from M. catarrhalis. Use of the predicted MapA translocation domain in a fusion construct with the passenger domain from another predicted M. catarrhalis autotransporter confirmed the translocation ability of this MapA domain. Inactivation of the mapA gene in M. catarrhalis strain O35E reduced the acid phosphatase activity expressed by this organism, and this mutation could be complemented in trans with the wild-type mapA gene. Nucleotide sequence analysis of the mapA gene from six M. catarrhalis strains showed that this protein was highly conserved among strains of this pathogen. Site-directed mutagenesis of a critical histidine residue (H233A) in the predicted active site of the acid phosphatase domain in MapA eliminated acid phosphatase activity in the recombinant MapA protein. This is the first description of an autotransporter protein that expresses acid phosphatase activity. PMID:18065547

  3. Use of the Chinchilla Model for Nasopharyngeal Colonization To Study Gene Expression by Moraxella catarrhalis

    PubMed Central

    Hoopman, Todd C.; Liu, Wei; Joslin, Stephanie N.; Pybus, Christine; Sedillo, Jennifer L.; Labandeira-Rey, Maria; Laurence, Cassie A.; Wang, Wei; Richardson, James A.; Bakaletz, Lauren O.

    2012-01-01

    Young adult chinchillas were atraumatically inoculated with Moraxella catarrhalis via the nasal route. Detailed histopathologic examination of nasopharyngeal tissues isolated from these M. catarrhalis-infected animals revealed the presence of significant inflammation within the epithelium. Absence of similar histopathologic findings in sham-inoculated animals confirmed that M. catarrhalis was exposed to significant host-derived factors in this environment. Twenty-four hours after inoculation, viable M. catarrhalis organisms were recovered from the nasal cavity and nasopharynx of the animals in numbers sufficient for DNA microarray analysis. More than 100 M. catarrhalis genes were upregulated in vivo, including open reading frames (ORFs) encoding proteins that are involved in a truncated denitrification pathway or in the oxidative stress response, as well as several putative transcriptional regulators. Additionally, 200 M. catarrhalis genes were found to be downregulated when this bacterium was introduced into the nasopharynx. These downregulated genes included ORFs encoding several well-characterized M. catarrhalis surface proteins including Hag, McaP, and MchA1. Real-time reverse transcriptase PCR (RT-PCR) was utilized as a stringent control to validate the results of in vivo gene expression patterns as measured by DNA microarray analysis. Inactivation of one of the genes (MC ORF 1550) that was upregulated in vivo resulted in a decrease in the ability of M. catarrhalis to survive in the chinchilla nasopharynx over a 3-day period. This is the first evaluation of global transcriptome expression by M. catarrhalis cells in vivo. PMID:22184412

  4. Comparison of Restriction Enzymes for Pulsed-Field Gel Electrophoresis Typing of Moraxella catarrhalis

    PubMed Central

    Puig, Carmen; Domenech, Arnau; Liñares, Josefina; Ardanuy, Carmen

    2013-01-01

    NotI, the most prevalent restriction enzyme used for typing Moraxella catarrhalis, failed to digest genomic DNA from respiratory samples. An improved pulsed-field gel electrophoresis (PFGE) methodology determined SpeI as the best choice for typing this bacterial species, with a good restriction of clinical samples and a good clustering correlation with NotI. PMID:23678064

  5. Moraxella catarrhalis Expresses a Cardiolipin Synthase That Impacts Adherence to Human Epithelial Cells

    PubMed Central

    Buskirk, Sean W.

    2014-01-01

    The major phospholipid constituents of Moraxella catarrhalis membranes are phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin (CL). However, very little is known regarding the synthesis and function of these phospholipids in M. catarrhalis. In this study, we discovered that M. catarrhalis expresses a cardiolipin synthase (CLS), termed MclS, that is responsible for the synthesis of CL within the bacterium. The nucleotide sequence of mclS is highly conserved among M. catarrhalis isolates and is predicted to encode a protein with significant amino acid similarity to the recently characterized YmdC/ClsC protein of Escherichia coli. Isogenic mclS mutant strains were generated in M. catarrhalis isolates O35E, O12E, and McGHS1 and contained no observable levels of CL. Site-directed mutagenesis of a highly conserved HKD motif of MclS also resulted in a CL-deficient strain. Moraxella catarrhalis, which depends on adherence to epithelial cells for colonization of the human host, displays significantly reduced levels of adherence to HEp-2 and A549 cell lines in the mclS mutant strains compared to wild-type bacteria. The reduction in adherence appears to be attributed to the absence of CL. These findings mark the first instance in which a CLS has been related to a virulence-associated trait. PMID:24142255

  6. Mining the Moraxella catarrhalis Genome: Identification of Potential Vaccine Antigens Expressed during Human Infection?

    PubMed Central

    Ruckdeschel, Elizabeth A.; Kirkham, Charmaine; Lesse, Alan J.; Hu, Zihua; Murphy, Timothy F.

    2008-01-01

    Moraxella catarrhalis is an important cause of respiratory infections in adults and otitis media in children. Developing an effective vaccine would reduce the morbidity, mortality, and costs associated with such infections. An unfinished genome sequence of a strain of M. catarrhalis available in the GenBank database was analyzed, and open reading frames predicted to encode potential vaccine candidates were identified. Three genes encoding proteins having molecular masses of approximately 22, 75, and 78 kDa (designated Msp [Moraxella surface proteins]) (msp22, msp75, and msp78, respectively) were determined to be conserved by competitive hybridization using a microarray, PCR, and sequencing of the genes in clinical isolates of M. catarrhalis. The genes were transcribed when M. catarrhalis was grown in vitro. These genes were amplified by PCR and cloned into Escherichia coli expression vectors. Recombinant proteins were generated and then studied using enzyme-linked immunosorbent assays with preacquisition and postclearance serum and sputum samples from 31 adults with chronic obstructive pulmonary disease (COPD) who acquired and cleared M. catarrhalis. New antibody responses to the three proteins were observed for a small proportion of the patients with COPD, indicating that these proteins were expressed during human infection. These studies indicate that the Msp22, Msp75, and Msp78 proteins, whose genes were discovered using genome mining, are highly conserved among strains, are expressed during human infection with M. catarrhalis, and represent potential vaccine antigens. PMID:18227159

  7. Moraxella catarrhalis uses a twin-arginine translocation system to secrete the ?-lactamase BRO-2

    PubMed Central

    2013-01-01

    Background Moraxella catarrhalis is a human-specific gram-negative bacterium readily isolated from the respiratory tract of healthy individuals. The organism also causes significant health problems, including 15-20% of otitis media cases in children and ~10% of respiratory infections in adults with chronic obstructive pulmonary disease. The lack of an efficacious vaccine, the rapid emergence of antibiotic resistance in clinical isolates, and high carriage rates reported in children are cause for concern. Virtually all Moraxella catarrhalis isolates are resistant to ?-lactam antibiotics, which are generally the first antibiotics prescribed to treat otitis media in children. The enzymes responsible for this resistance, BRO-1 and BRO-2, are lipoproteins and the mechanism by which they are secreted to the periplasm of M. catarrhalis cells has not been described. Results Comparative genomic analyses identified M. catarrhalis gene products resembling the TatA, TatB, and TatC proteins of the well-characterized Twin Arginine Translocation (TAT) secretory apparatus. Mutations in the M. catarrhalis tatA, tatB and tatC genes revealed that the proteins are necessary for optimal growth and resistance to ?-lactams. Site-directed mutagenesis was used to replace highly-conserved twin arginine residues in the predicted signal sequence of M. catarrhalis strain O35E BRO-2, which abolished resistance to the ?-lactam antibiotic carbanecillin. Conclusions Moraxella catarrhalis possesses a TAT secretory apparatus, which plays a key role in growth of the organism and is necessary for secretion of BRO-2 into the periplasm where the enzyme can protect the peptidoglycan cell wall from the antimicrobial activity of ?-lactam antibiotics. PMID:23782650

  8. The ability to bind albumin is correlated with nitric oxide sensitivity in Moraxella catarrhalis.

    PubMed

    Maluszynska, G M; Krachler, B; Sundqvist, T

    1998-09-15

    Moraxella catarrhalis is sensitive to NO generators, e.g. S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SNP), but can spontaneously develop higher SNP tolerance. Using SDS-PAGE of outer membrane proteins and immunoblotting for serum albumin, we found that the wild strain bound more blood-medium-derived albumin than the SNP-resistant variant did. There was a negative correlation between NO tolerance and the presence of serum albumin in the medium. We suggest that M. catarrhalis can change its surface properties to avoid binding albumin and thereby increase its resistance to NO. Growth of Moraxella is affected by iron, and that may have influenced our results. Using chrome azurol S plates as an indicator, we noted that both albumin and SNP have a strong affinity for iron(III). PMID:9770282

  9. The ability to bind albumin is correlated with nitric oxide sensitivity in Moraxella catarrhalis

    Microsoft Academic Search

    Gizela M. Maluszynska; Benno Krachler; Tommy Sundqvist

    1998-01-01

    Moraxella catarrhalis is sensitive to NO generators e.g. S-nitroso-N-acetylpenicillamine (SNAP) and sodium nitroprusside (SNP), but can spontaneously develop higher SNP tolerance. Using SDS-PAGE of outer membrane proteins and immunoblotting for serum albumin, we found that the wild strain bound more blood-medium-derived albumin than the SNP-resistant variant did. There was a negative correlation between NO tolerance and the presence of serum

  10. Antigenic specificity of the mucosal antibody response to Moraxella catarrhalis in chronic obstructive pulmonary disease.

    PubMed

    Murphy, Timothy F; Brauer, Aimee L; Aebi, Christoph; Sethi, Sanjay

    2005-12-01

    Moraxella catarrhalis is an important human mucosal pathogen causing otitis media in children and lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). Little is known about the mucosal antibody response to M. catarrhalis in adults with COPD. In this study, 10 pairs of well-characterized sputum supernatant samples from adults with COPD who had acquired and subsequently cleared M. catarrhalis from their respiratory tracts were studied in detail in an effort to begin to elucidate potentially protective immune responses. Flow cytometry analysis was used to study the distribution of immunoglobulin isotypes in paired preacquisition and postclearance sputum samples. The results showed that immunoglobulin A (IgA) is the predominant M. catarrhalis-specific immunoglobulin isotype and that the sputum IgA contains a secretory component, indicating that it is locally produced at the mucosal site. Most patients made new sputum IgA responses to the adhesins UspA1 and Hag, along with the surface protein UspA2. A smaller proportion of patients made new sputum IgA responses to the iron-regulated proteins TbpB and CopB and to lipooligosaccharide. These results have important implications in understanding the mucosal immune response to M. catarrhalis in the setting of COPD and in elucidating the elements of a protective immune response. PMID:16299311

  11. Characterization of the Molecular Interplay between Moraxella catarrhalis and Human Respiratory Tract Epithelial Cells

    PubMed Central

    de Vries, Stefan P. W.; Eleveld, Marc J.; Hermans, Peter W. M.; Bootsma, Hester J.

    2013-01-01

    Moraxella catarrhalis is a mucosal pathogen that causes childhood otitis media and exacerbations of chronic obstructive pulmonary disease in adults. During the course of infection, M. catarrhalis needs to adhere to epithelial cells of different host niches such as the nasopharynx and lungs, and consequently, efficient adhesion to epithelial cells is considered an important virulence trait of M. catarrhalis. By using Tn-seq, a genome-wide negative selection screenings technology, we identified 15 genes potentially required for adherence of M. catarrhalis BBH18 to pharyngeal epithelial Detroit 562 and lung epithelial A549 cells. Validation with directed deletion mutants confirmed the importance of aroA (3-phosphoshikimate 1-carboxyvinyl-transferase), ecnAB (entericidin EcnAB), lgt1 (glucosyltransferase), and MCR_1483 (outer membrane lipoprotein) for cellular adherence, with ?MCR_1483 being most severely attenuated in adherence to both cell lines. Expression profiling of M. catarrhalis BBH18 during adherence to Detroit 562 cells showed increased expression of 34 genes in cell-attached versus planktonic bacteria, among which ABC transporters for molybdate and sulfate, while reduced expression of 16 genes was observed. Notably, neither the newly identified genes affecting adhesion nor known adhesion genes were differentially expressed during adhesion, but appeared to be constitutively expressed at a high level. Profiling of the transcriptional response of Detroit 562 cells upon adherence of M. catarrhalis BBH18 showed induction of a panel of pro-inflammatory genes as well as genes involved in the prevention of damage of the epithelial barrier. In conclusion, this study provides new insight into the molecular interplay between M. catarrhalis and host epithelial cells during the process of adherence. PMID:23936538

  12. Connection between Trimethoprim-Sulfamethoxazole Use and Resistance in Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis?

    PubMed Central

    Kärpänoja, Pauliina; Nyberg, Solja T.; Bergman, Miika; Voipio, Tinna; Paakkari, Pirkko; Huovinen, Pentti; Sarkkinen, Hannu

    2008-01-01

    The association between trimethoprim-sulfamethoxazole use and resistance among the major respiratory tract pathogens was investigated by comparing regional consumption of the drug to regional resistance in the following year in 21 central hospital districts in Finland. A total of 23,530 Streptococcus pneumoniae isolates, 28,320 Haemophilus influenzae isolates, and 14,138 Moraxella catarrhalis isolates were tested for trimethoprim-sulfamethoxazole susceptibility during the study period (1998-2004). Among the S. pneumoniae isolates, a statistically significant connection was found between regional consumption and resistance. No statistically significant connection was found between regional trimethoprim-sulfamethoxazole use and resistance among H. influenzae and M. catarrhalis isolates. According to our results, it seems that only in pneumococci can the development of trimethoprim-sulfamethoxazole resistance be influenced by restricting its use. However, trimethoprim-sulfamethoxazole remains an important antimicrobial agent because of its reasonable price. Hence, resistance to trimethoprim-sulfamethoxazole among these pathogens needs continuous monitoring. PMID:18443116

  13. Non-transpeptidase binding arylthioether ?-lactams active against Mycobacterium tuberculosis and Moraxella catarrhalis.

    PubMed

    Beck, Tim N; Lloyd, Dina; Kuskovsky, Rostislav; Minah, Jeanette; Arora, Kriti; Plotkin, Balbina J; Green, Jacalyn M; Boshoff, Helena I; Barry, Clifton; Deschamps, Jeffrey; Konaklieva, Monika I

    2015-02-01

    The prevalence of drug resistance in both clinical and community settings as a consequence of alterations of biosynthetic pathways, enzymes or cell wall architecture is a persistent threat to human health. We have designed, synthesized, and tested a novel class of non-transpeptidase, ?-lactamase resistant monocyclic ?-lactams that carry an arylthio group at C4. These thioethers exhibit inhibitory and cidal activity against serine ?-lactamase producing Mycobacterium tuberculosis wild type strain (Mtb) and multiple (n=8) ?-lactamase producing Moraxella catarrhalis clinical isolates. PMID:25549898

  14. Indirect Pathogenicity of Haemophilus influenzae and Moraxella catarrhalis in Polymicrobial Otitis Media Occurs via Interspecies Quorum Signaling

    PubMed Central

    Armbruster, Chelsie E.; Hong, Wenzhou; Pang, Bing; Weimer, Kristin E. D.; Juneau, Richard A.; Turner, James; Swords, W. Edward

    2010-01-01

    Otitis media (OM) is among the leading diseases of childhood and is caused by opportunists that reside within the nasopharynx, such as Haemophilus influenzae and Moraxella catarrhalis. As with most airway infections, it is now clear that OM infections involve multiple organisms. This study addresses the hypothesis that polymicrobial infection alters the course, severity, and/or treatability of OM disease. The results clearly show that coinfection with H. influenzae and M. catarrhalis promotes the increased resistance of biofilms to antibiotics and host clearance. Using H. influenzae mutants with known biofilm defects, these phenotypes were shown to relate to biofilm maturation and autoinducer-2 (AI-2) quorum signaling. In support of the latter mechanism, chemically synthesized AI-2 (dihydroxypentanedione [DPD]) promoted increased M. catarrhalis biofilm formation and resistance to antibiotics. In the chinchilla infection model of OM, polymicrobial infection promoted M. catarrhalis persistence beyond the levels seen in animals infected with M. catarrhalis alone. Notably, no such enhancement of M. catarrhalis persistence was observed in animals infected with M. catarrhalis and a quorum signaling-deficient H. influenzae luxS mutant strain. We thus conclude that H. influenzae promotes M. catarrhalis persistence within polymicrobial biofilms via interspecies quorum signaling. AI-2 may therefore represent an ideal target for disruption of chronic polymicrobial infections. Moreover, these results strongly imply that successful vaccination against the unencapsulated H. influenzae strains that cause airway infections may also significantly impact chronic M. catarrhalis disease by removing a reservoir of the AI-2 signal that promotes M. catarrhalis persistence within biofilm. PMID:20802829

  15. Genetic analysis of a pediatric clinical isolate of Moraxella catarrhalis with resistance to macrolides and quinolones.

    PubMed

    Iwata, Satoshi; Sato, Yoshitake; Toyonaga, Yoshikiyo; Hanaki, Hideaki; Sunakawa, Keisuke

    2015-04-01

    During the surveillance conducted in 2012 by the Drug-resistant Pathogen Surveillance Group in Pediatric Infectious Disease, we isolated a strain of Moraxella catarrhalis that demonstrated resistance to both macrolides and quinolones from a male pediatric patient aged 1.5 years who had developed acute bronchitis. Then we evaluated the susceptibility of this strain to different types of antibacterial agents and conducted a genetic analysis. The results of the susceptibility evaluation showed that the MIC values of azithromycin, clarithromycin, and rokitamycin were >64 ?g/mL, >64 ?g/mL, and 4 ?g/mL, respectively; clearly demonstrating resistance to macrolides. The MIC values of the quinolones levofloxacin, tosufloxacin, and garenoxacin were 4 ?g/mL, 2 ?g/mL, and 1 ?g/mL, respectively; indicating decreased susceptibility. The genetic analysis of this strain revealed one mutation in 23s rRNA with a replacement of adenine by thymine at nucleotide position 2330 (A2330T) and another mutation in gyrB at nucleotide position 1481 by replacement of adenine with guanine (A1481G) that caused a substitution of the 494 th asparagine acid by glycine, as being associated with the observed resistance to macrolides and quinolones, respectively. Similar to drug-resistant bacteria Streptococcus pneumoniae and Haemophilus influenzae, the prevalence of which has recently increased, the treatment of drug-resistant M. catarrhalis infections is considered difficult due to the development of resistance to different types of antibacterial agents. It is vital to maintain an unwavering focus on the trend toward an increasing number of drug-resistant M. catarrhalis strains and ensure the proper use of each antibacterial agent. PMID:25481761

  16. Construction and Characterization of Moraxella catarrhalis Mutants Defective in Expression of Transferrin Receptors

    PubMed Central

    Luke, Nicole R.; Campagnari, Anthony A.

    1999-01-01

    We have previously reported the construction of an isogenic mutant defective in expression of OmpB1, the TbpB homologue, in Moraxella catarrhalis 7169. In this report, we have extended these studies by constructing and characterizing two new isogenic mutants in this clinical isolate. One mutant is defective in expression of TbpA, and the other mutant is defective in expression of both TbpA and TbpB. These isogenic mutants were confirmed by using PCR analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and sequencing. In vitro growth studies, comparing all three mutants, demonstrated that the tbpA mutant and the tbpAB mutant were severely limited in their ability to grow with human holotransferrin as the sole source of iron. In contrast, the ompB1 (tbpB) mutant was capable of utilizing iron from human transferrin, although not to the extent of the parental strain. While affinity chromatography with human holotransferrin showed that each Tbp was capable of binding independently to transferrin, solid-phase transferrin binding studies using whole cells demonstrated that the tbpA mutant exhibited binding characteristics similar to those seen with the wild-type bacteria. However, the ompB1 (tbpB) mutant exhibited a diminished capacity for binding transferrin, and no binding was detected with the double mutant. These data suggest that the M. catarrhalis TbpA is necessary for the acquisition of iron from transferrin. In contrast, TbpB is not essential but may serve as a facilitory protein that functions to optimize this process. Together these mutants are essential to provide a more thorough understanding of iron acquisition mechanisms utilized by M. catarrhalis. PMID:10531234

  17. Residence of Streptococcus pneumoniae and Moraxella catarrhalis within polymicrobial biofilm promotes antibiotic resistance and bacterial persistence in vivo

    PubMed Central

    Perez, Antonia C.; Pang, Bing; King, Lauren B.; Tan, Li; Murrah, Kyle A.; Reimche, Jennifer L.; Wren, John T.; Richardson, Stephen H.; Ghandi, Uma; Swords, W. Edward

    2014-01-01

    Otitis media is an extremely common pediatric ailment caused by opportunists that reside within the nasopharynx. Inflammation within the upper airway can promote ascension of these opportunists into the middle ear chamber. Otitis media can be chronic/recurrent in nature, and a wealth of data indicates that in these cases the bacteria persist within biofilms. Epidemiological data demonstrates most cases of otitis media are polymicrobial, which may have significant impact on antibiotic resistance. In this study, we used in vitro biofilm assays and rodent infection models to examine the impact of polymicrobial infection with Moraxella catarrhalis and Streptococcus pneumoniae (pneumococcus) on biofilm resistance to antibiotic treatment and persistence in vivo. Consistent with prior work, M. catarrhalis conferred beta-lactamase dependent passive protection from beta-lactam killing to pneumococci within polymicrobial biofilms. Moreover, pneumococci increased resistance of M. catarrhalis to macrolide killing in polymicrobial biofilms. However, pneumococci increased colonization in vivo by M. catarrhalis in a quorum signal-dependent manner. We also found that co-infection with M. catarrhalis affects middle ear ascension of pneumococci in both mice and chinchillas. Therefore, we conclude that residence of M. catarrhalis and pneumococci within the same biofilm community significantly impacts resistance to antibiotic treatment and bacterial persistence in vivo. PMID:24391058

  18. Substrate Binding Protein SBP2 of a Putative ABC Transporter as a Novel Vaccine Antigen of Moraxella catarrhalis

    PubMed Central

    Otsuka, Taketo; Kirkham, Charmaine; Johnson, Antoinette; Jones, Megan M.

    2014-01-01

    Moraxella catarrhalis is a common respiratory tract pathogen that causes otitis media in children and infections in adults with chronic obstructive pulmonary disease. Since the introduction of the pneumococcal conjugate vaccines with/without protein D of nontypeable Haemophilus influenzae, M. catarrhalis has become a high-priority pathogen in otitis media. For the development of antibacterial vaccines and therapies, substrate binding proteins of ATP-binding cassette transporters are important targets. In this study, we identified and characterized a substrate binding protein, SBP2, of M. catarrhalis. Among 30 clinical isolates tested, the sbp2 gene sequence was highly conserved. In 2 different analyses (whole-cell enzyme-linked immunosorbent assay and flow cytometry), polyclonal antibodies raised to recombinant SBP2 demonstrated that SBP2 expresses epitopes on the bacterial surface of the wild type but not the sbp2 mutant. Mice immunized with recombinant SBP2 showed significantly enhanced clearance of M. catarrhalis from the lung compared to that in the control group at both 25-?g and 50-?g doses (P < 0.001). We conclude that SBP2 is a novel, attractive candidate as a vaccine antigen against M. catarrhalis. PMID:24914218

  19. Differential Virulence Gene Expression of Group A Streptococcus Serotype M3 in Response to Co-Culture with Moraxella catarrhalis

    PubMed Central

    Verhaegh, Suzanne J. C.; Flores, Anthony R.; van Belkum, Alex; Musser, James M.; Hays, John P.

    2013-01-01

    Streptococcus pyogenes (group A Streptococcus, GAS) and Moraxella catarrhalis are important colonizers and (opportunistic) pathogens of the human respiratory tract. However, current knowledge regarding colonization and pathogenic potential of these two pathogens is based on work involving single bacterial species, even though the interplay between respiratory bacterial species is increasingly important in niche occupation and the development of disease. Therefore, to further define and understand polymicrobial species interactions, we investigated whether gene expression (and hence virulence potential) of GAS would be affected upon co-culture with M. catarrhalis. For co-culture experiments, GAS and M. catarrhalis were cultured in Todd-Hewitt broth supplemented with 0.2% yeast extract (THY) at 37°C with 5% CO2 aeration. Each strain was grown in triplicate so that triplicate experiments could be performed. Bacterial RNA was isolated, cDNA synthesized, and microarray transcriptome expression analysis performed. We observed significantly increased (?4-fold) expression for genes playing a role in GAS virulence such as hyaluronan synthase (hasA), streptococcal mitogenic exotoxin Z (smeZ) and IgG endopeptidase (ideS). In contrast, significantly decreased (?4-fold) expression was observed in genes involved in energy metabolism and in 12 conserved GAS two-component regulatory systems. This study provides the first evidence that M. catarrhalis increases GAS virulence gene expression during co-culture, and again shows the importance of polymicrobial infections in directing bacterial virulence. PMID:23626831

  20. The UspA1 Protein and a Second Type of UspA2 Protein Mediate Adherence of Moraxella catarrhalis to Human Epithelial Cells In Vitro

    Microsoft Academic Search

    ERIC R. LAFONTAINE; LESLIE D. COPE; CHRISTOPH AEBI; JO L. LATIMER; G. H. McCracken; E. J. Hansen

    2000-01-01

    The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicated that UspA1 was involved in adherence of this organism to Chang conjunctival epithelial cells in vitro and

  1. Oropharyngeal Colonization With Neisseria lactamica, Other Nonpathogenic Neisseria Species and Moraxella catarrhalis Among Young Healthy Children in Ahvaz, Iran

    PubMed Central

    Sheikhi, Raheleh; Amin, Mansour; Rostami, Soodabeh; Shoja, Saeed; Ebrahimi, Nasim

    2015-01-01

    Background: Neisseria lactamica as one of the main commensal in oropharynx during the childhood is related to the induction of a natural immunity against meningococcal meningitis. Also Moraxella catarrhalis in oropharynx of children is a predisposing factor for otitis media infection. Objectives: The current study aimed to investigate the frequency of the N. lactamica, other nonpathogenic Neisseria spp. and M. catarrhalis in the oropharynx of young healthy children in Ahvaz, Iran by the two phenotypic tests and Polymerase Chain Reaction (PCR). Materials and Methods: A total of 192 oropharyngeal swab samples of the young healthy children were studied during four months. Swabs were plated onto enriched selective media and non-selective media. Gram-negative and oxidase-positive diplococci were identified by several conventional biochemical tests. The PCR and sequencing were used to confirm the accuracy of laboratory diagnosis to identify N. lactamica and M. catarrhalis. Results: Among 192 young healthy children with the mean age of 5.93 ± 2.5903 years, authors identified: N. lactamica (21.9%) in the age group of one to nine years; N. mucosa (6.3%); N. sicca (7.8%); N. cinerea (1.6%); N. subflava (biovar subflava) (4.2%); N. subflava (biovar perflava) (28.1%); N. subflava (biovar flava) (7.3%) and M. catarrhalis (42.7%). Conclusions: The young healthy children screening by colonization of N. lactamica and other nonpathogenic Neisseria spp. in oropharynx was the first report in Ahvaz, Iran. The study results demonstrated the high frequency of colonization of M. catarrhalis in the studied young healthy children other than Neisseria spp. PMID:25964847

  2. Long-term survival of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis as isolates and in nasopharyngeal specimens in frozen STGG storage medium.

    PubMed

    Kaijalainen, Tarja; Palmu, Arto

    2015-07-01

    We evaluated survival in WHO-recommended STGG storage medium of bacteria causing respiratory-tract infection. Streptococcus pneumoniae and Moraxella catarrhalis survived as single and mixed isolates stored at -70°C for 12.5years, but Haemophilus influenzae less than 4years. All the bacteria survived in the nasopharyngeal specimens at -70°C for 11years. PMID:25937246

  3. Increased recovery of Moraxella catarrhalis and Haemophilus influenzae in association with group A  -haemolytic streptococci in healthy children and those with pharyngo-tonsillitis

    Microsoft Academic Search

    Itzhak Brook; Alan E. Gober

    2006-01-01

    The inflamed tonsils harbour numerous types of bacteria, alone or in combination with group A b-haemolytic streptococci (GABHS). The cohabitation of the tonsils by GABHS and certain other bacterial species may contribute to the inflammatory process and the failure of penicillin therapy. This study evaluated the recovery of Moraxella catarrhalis, Haemophilus influenzae, Staphylococcus aureus and Streptococcus pneumoniae in association with

  4. Comparative analysis and supragenome modeling of twelve Moraxella catarrhalis clinical isolates

    Microsoft Academic Search

    Jeremiah J Davie; Josh Earl; Stefan PW de Vries; Azad Ahmed; Fen Z Hu; Hester J Bootsma; Kim Stol; Peter WM Hermans; Robert M Wadowsky; Garth D Ehrlich; John P Hays; Anthony A Campagnari

    2011-01-01

    BACKGROUND: M. catarrhalis is a gram-negative, gamma-proteobacterium and an opportunistic human pathogen associated with otitis media (OM) and exacerbations of chronic obstructive pulmonary disease (COPD). With direct and indirect costs for treating these conditions annually exceeding $33 billion in the United States alone, and nearly ubiquitous resistance to beta-lactam antibiotics among M. catarrhalis clinical isolates, a greater understanding of this

  5. A major outer membrane protein of Moraxella catarrhalis is a target for antibodies that enhance pulmonary clearance of the pathogen in an animal model.

    PubMed Central

    Helminen, M E; Maciver, I; Latimer, J L; Cope, L D; McCracken, G H; Hansen, E J

    1993-01-01

    A murine immunoglobulin G monoclonal antibody (MAb) raised against outer membrane vesicles of Moraxella catarrhalis 035E was shown to bind to a surface-exposed epitope of a major outer membrane protein of this organism. This outer membrane protein, which had an apparent molecular weight of approximately 80,000 in sodium dodecyl sulfate-polyacrylamide gels, was designated CopB. MAb 10F3, reactive with CopB, bound to a majority (70%) of M. catarrhalis strains tested. More importantly, mice passively immunized with MAb 10F3 exhibited an enhanced ability to clear a bolus challenge of M. catarrhalis from their lungs, a result which suggested that CopB might have potential as a vaccine candidate. The M. catarrhalis gene encoding CopB was cloned in Escherichia coli, and nucleotide sequence analysis of the copB gene indicated that the CopB protein was synthesized with a leader peptide, a finding confirmed by N-terminal amino acid sequence analysis of the mature CopB protein purified from M. catarrhalis 035E. Southern blot analysis showed that chromosomal DNA from seven different M. catarrhalis strains hybridized with a probe comprising the majority of the copB structural gene from strain 035E. Additional data emphasizing the extent of conservation of the CopB protein among M. catarrhalis strains were obtained from Western immunoblot analyses with polyclonal antisera raised against CopB proteins from different M. catarrhalis strains used to probe the recombinant form of the CopB protein from strain 035E. The ability of the CopB protein to function as a target for biologically active antibodies and its apparent conservation among M. catarrhalis strains warrant further investigation of this outer membrane protein as a potential vaccine candidate. Images PMID:7683000

  6. EXPRESSION OF THE COPB OUTER MEMBRANE PROTEIN BY MORAXELLA CATARRHALIS IS REGULATED BY IRON AND AFFECTS IRON ACQUISITION FROM TRANSFERRIN AND LACTOFERRIN. ??? 974

    Microsoft Academic Search

    Christoph Aebi; Barbara Stone; Margaret Beucher; Leslie D. Cope; Isobel Maciver; Sharon E. Thomas; P. Frederick Sparling; Eric J. Hansen

    1996-01-01

    The amino acid sequence of the cell-surface-exposed, 81-kDa CopB outer membrane protein of Moraxella catarrhalis was found to be similar to those of TonB-dependent outer membrane proteins of other gram- negative bacteria. Expression of CopB was affected by the availability of iron in the growth medium, and the extent of overexpression of CopB in response to iron limitation varied widely

  7. Use of the Chinchilla Model to Evaluate the Vaccinogenic Potential of the Moraxella catarrhalis Filamentous Hemagglutinin-like Proteins MhaB1 and MhaB2

    PubMed Central

    Shaffer, Teresa L.; Balder, Rachel; Buskirk, Sean W.; Hogan, Robert J.; Lafontaine, Eric R.

    2013-01-01

    Moraxella catarrhalis causes significant health problems, including 15–20% of otitis media cases in children and ?10% of respiratory infections in adults with chronic obstructive pulmonary disease. The lack of an efficacious vaccine, the rapid emergence of antibiotic resistance in clinical isolates, and high carriage rates reported in children are cause for concern. In addition, the effectiveness of conjugate vaccines at reducing the incidence of otitis media caused by Streptococcus pneumoniae and nontypeable Haemophilus influenzae suggest that M. catarrhalis infections may become even more prevalent. Hence, M. catarrhalis is an important and emerging cause of infectious disease for which the development of a vaccine is highly desirable. Studying the pathogenesis of M. catarrhalis and the testing of vaccine candidates have both been hindered by the lack of an animal model that mimics human colonization and infection. To address this, we intranasally infected chinchilla with M. catarrhalis to investigate colonization and examine the efficacy of a protein-based vaccine. The data reveal that infected chinchillas produce antibodies against antigens known to be major targets of the immune response in humans, thus establishing immune parallels between chinchillas and humans during M. catarrhalis infection. Our data also demonstrate that a mutant lacking expression of the adherence proteins MhaB1 and MhaB2 is impaired in its ability to colonize the chinchilla nasopharynx, and that immunization with a polypeptide shared by MhaB1 and MhaB2 elicits antibodies interfering with colonization. These findings underscore the importance of adherence proteins in colonization and emphasize the relevance of the chinchilla model to study M. catarrhalis–host interactions. PMID:23844117

  8. Use of an Isogenic Mutant Constructed in Moraxella catarrhalis To Identify a Protective Epitope of Outer Membrane Protein B1 Defined by Monoclonal Antibody 11C6

    PubMed Central

    Luke, Nicole R.; Russo, Thomas A.; Luther, Neal; Campagnari, Anthony A.

    1999-01-01

    Moraxella catarrhalis-induced otitis media continues to be a significant cause of infection in young children, prompting increased efforts at identifying effective vaccine antigens. We have previously demonstrated that M. catarrhalis expresses specific outer membrane proteins (OMPs) in response to iron limitation and that this organism can utilize transferrin and lactoferrin for in vitro growth. One of these proteins, which binds human transferrin, is OMP B1. As the human host presents a naturally iron-limited environment, proteins, like OMP B1, which are expressed in response to this nutritional stress are potential vaccine antigens. In this study, we have developed monoclonal antibody (MAb) 11C6, which reacts to a surface-exposed epitope of OMP B1 expressed by M. catarrhalis 7169. This antibody was used to clone ompB1, and sequence analysis suggested that OMP B1 is the M. catarrhalis homologue to the transferrin binding protein B described for pathogenic Neisseriaceae, Haemophilus influenzae, Actinobacillus pleuropneumoniae, and M. catarrhalis. Expression of recombinant OMP B1 on the surface of Escherichia coli confers transferrin binding activity, confirming that this protein is likely involved in iron acquisition. In addition, ompB1 was used to construct an isogenic mutant in M. catarrhalis 7169. This mutant, termed 7169b12, was used as the control in bactericidal assays designed to determine if OMP B1 elicits protective antibodies. In the presence of MAb 11C6 and human complement, wild-type 7169 demonstrated a 99% decline in viability, whereas the ompB1 isogenic mutant was resistant to this bactericidal activity. Further analysis with MAb 11C6 revealed the presence of this OMP B1 epitope on 31% of the clinical isolates tested. These data suggest that OMP B1 is a potential vaccine antigen against M. catarrhalis infections. PMID:9916077

  9. A Moraxella catarrhalis Two-Component Signal Transduction System Necessary for Growth in Liquid Media Affects Production of Two Lysozyme Inhibitors

    PubMed Central

    Joslin, Stephanie N.; Pybus, Christine; Labandeira-Rey, Maria; Evans, Amanda S.; Attia, Ahmed S.; Brautigam, Chad A.

    2014-01-01

    There are a paucity of data concerning gene products that could contribute to the ability of Moraxella catarrhalis to colonize the human nasopharynx. Inactivation of a gene (mesR) encoding a predicted response regulator of a two-component signal transduction system in M. catarrhalis yielded a mutant unable to grow in liquid media. This mesR mutant also exhibited increased sensitivity to certain stressors, including polymyxin B, SDS, and hydrogen peroxide. Inactivation of the gene (mesS) encoding the predicted cognate sensor (histidine) kinase yielded a mutant with the same inability to grow in liquid media as the mesR mutant. DNA microarray and real-time reverse transcriptase PCR analyses indicated that several genes previously shown to be involved in the ability of M. catarrhalis to persist in the chinchilla nasopharynx were upregulated in the mesR mutant. Two other open reading frames upregulated in the mesR mutant were shown to encode small proteins (LipA and LipB) that had amino acid sequence homology to bacterial adhesins and structural homology to bacterial lysozyme inhibitors. Inactivation of both lipA and lipB did not affect the ability of M. catarrhalis O35E to attach to a human bronchial epithelial cell line in vitro. Purified recombinant LipA and LipB fusion proteins were each shown to inhibit human lysozyme activity in vitro and in saliva. A lipA lipB deletion mutant was more sensitive than the wild-type parent strain to killing by human lysozyme in the presence of human apolactoferrin. This is the first report of the production of lysozyme inhibitors by M. catarrhalis. PMID:25312959

  10. A Moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors.

    PubMed

    Joslin, Stephanie N; Pybus, Christine; Labandeira-Rey, Maria; Evans, Amanda S; Attia, Ahmed S; Brautigam, Chad A; Hansen, Eric J

    2015-01-01

    There are a paucity of data concerning gene products that could contribute to the ability of Moraxella catarrhalis to colonize the human nasopharynx. Inactivation of a gene (mesR) encoding a predicted response regulator of a two-component signal transduction system in M. catarrhalis yielded a mutant unable to grow in liquid media. This mesR mutant also exhibited increased sensitivity to certain stressors, including polymyxin B, SDS, and hydrogen peroxide. Inactivation of the gene (mesS) encoding the predicted cognate sensor (histidine) kinase yielded a mutant with the same inability to grow in liquid media as the mesR mutant. DNA microarray and real-time reverse transcriptase PCR analyses indicated that several genes previously shown to be involved in the ability of M. catarrhalis to persist in the chinchilla nasopharynx were upregulated in the mesR mutant. Two other open reading frames upregulated in the mesR mutant were shown to encode small proteins (LipA and LipB) that had amino acid sequence homology to bacterial adhesins and structural homology to bacterial lysozyme inhibitors. Inactivation of both lipA and lipB did not affect the ability of M. catarrhalis O35E to attach to a human bronchial epithelial cell line in vitro. Purified recombinant LipA and LipB fusion proteins were each shown to inhibit human lysozyme activity in vitro and in saliva. A lipA lipB deletion mutant was more sensitive than the wild-type parent strain to killing by human lysozyme in the presence of human apolactoferrin. This is the first report of the production of lysozyme inhibitors by M. catarrhalis. PMID:25312959

  11. Regional Trends in Antimicrobial Resistance among Clinical Isolates of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in the United States: Results from the TRUST Surveillance Program, 1999–2000

    Microsoft Academic Search

    Clyde Thornsberry; Daniel F. Sahm; Laurie J. Kelly; Ian A. Critchley; Mark E. Jones; Alan T. Evangelista; James A. Karlowsky

    2002-01-01

    The ongoing TRUST (Tracking Resistance in the United States Today) study, which began monitoring anti- microbial resistance among respiratory pathogens in 1996, routinely tracks resistance at national and regional levels. The 1999-2000 TRUST study analyzed 9499 Streptococcus pneumoniae, 1934 Haemophilus influenzae, and 1108 Moraxella catarrhalis isolates that were prospectively collected from 239 laboratories across the 9 US Bureau of the

  12. Moraxella catarrhalis Activates Murine Macrophages through Multiple Toll Like Receptors and Has Reduced Clearance in Lungs from TLR4 Mutant Mice

    PubMed Central

    Hassan, Ferdaus; Ren, Dabin; Zhang, Wenhong; Merkel, Tod J.; Gu, Xin-Xing

    2012-01-01

    Moraxella catarrhalis is a Gram negative bacterium and a leading causative agent of otitis media (OM) in children. Several recent reports have provided strong evidence for an association between toll like receptors and OM. It has been found that both Streptococcus pneumoniae and nontypeable Haemophilus influenzae activate host protective immune responses through toll like receptors (TLRs), however, the precise mechanism by which Moraxella catarrhalis initiates the host immune response is currently unknown. In this report, using murine macrophages generated from a series of knock-out mice, we have demonstrated that M. catarrhalis lipooligosaccharide (LOS) and either heat killed or live bacteria are recognized by one or more TLRs. LOS activates the host immune response through a membrane bound CD14-TLR4 complex, while both heat killed and live M.cat require recognition by multiple toll like receptors such as TLR2, TLR4 and TLR9 without the requirement of CD14. We have also shown that M.cat stimuli are capable of triggering the host innate immune response by both MyD88- and TRIF- dependent signaling pathways. We further showed that M.cat induced activation of mitogen activated protein kinase (MAPK) is essential in order to achieve optimal secretion of pro-inflammatory cytokine TNF-?. We finally showed that TLR4 mutant C3H/HeJ mice produce significantly lower levels of pro-inflammatory cytokines TNF-? and IL-6 in vivo, An increased bacterial loads at 12 and 24 hours (P<0.001) in their lungs upon challenge with live M.cat in an aerosol chamber compared to wild-type (WT) control mice. These data suggest that TLRs are crucial for an effective innate immune response induced by M.cat. The results of these studies contribute to an increased understanding of molecular mechanism and possible novel treatment strategies for diseases caused by M.cat by specifically targeting TLRs and their signaling pathways. PMID:22662179

  13. Effect of 7-valent pneumococcal conjugate vaccine on nasopharyngeal carriage with Haemophilus influenzae and Moraxella catarrhalis in a randomized controlled trial.

    PubMed

    van Gils, Elske J M; Veenhoven, Reinier H; Rodenburg, Gerwin D; Hak, Eelko; Sanders, Elisabeth A M

    2011-10-13

    Seven-valent CRM197-conjugated pneumococcal conjugate vaccine (PCV7(CRM197)) reduces both vaccine serotype nasopharyngeal colonization and vaccine serotype acute otitis media by 50-60%. However, overall pneumococcal carriage and impact on otitis media are partly offset by concomitant increase of nonvaccine serotypes. We investigated in a randomized controlled trial the impact of 2-doses and 2+1-doses of PCV7(CRM197) on carriage of Streptococcus pneumoniae and of other nasopharyngeal commensals and well-known otitis media pathogens, Haemophilus influenzae and Moraxella catarrhalis, in children. Nasopharyngeal swabs were collected at the age of 6 weeks and at 6, 12, 18 and 24 months. We observed high carriage rates up to 68% for S. pneumoniae, 71% for H. influenzae and 68% for M. catarrhalis at the age of 18 months. Reduced dose (CRM197) schedules induced a slight reduction in overall pneumococcal carriage but no increases in the presence of H. influenzae and M. catarrhalis. PMID:21893151

  14. Impact of sequence diversity in the Moraxella catarrhalis UspA2/UspA2H head domain on vitronectin binding and antigenic variation.

    PubMed

    Su, Yu-Ching; Hallström, Björn M; Bernhard, Sara; Singh, Birendra; Riesbeck, Kristian

    2013-05-01

    The nasopharyngeal pathogen Moraxella catarrhalis recruits vitronectin to subvert complement-mediated killing. Ubiquitous surface protein (UspA) 2 and its hybrid form UspA2H bind vitronectin at the highly diverse N-terminal head domain. Here we characterized the sequence diversity of the head domain in multiple M. catarrhalis clinical isolates (n = 51) with focus on binding of vitronectin. The head domain of the uspA2 genes from 40 isolates were clustered according to an N-terminal sequence motif of UspA2 (NTER2), i.e., NTER2A (55% of uspA2 variants), NTER2B (32.5%), NTER2C (5%), and finally a group without an NTER2 (7.5%). Isolates harbouring the uspA2H gene (n = 11) contained N-terminal GGG repeats. Vitronectin binding to isolates having UspA2 did not correlate to variation in the NTER2 motifs but occurred in UspA2H containing 6 or ?11 of GGG repeats. Analyses of recombinant UspA2/UspA2H head domains of multiple variants showed UspA2-dependent binding to the C-terminal of vitronectin. Furthermore, polyclonal anti-UspA2 antibodies revealed that the head domain of the majority of Moraxella UspA2/2H was antigenically unrelated, whereas the full length molecules were recognized. In conclusion, the head domains of UspA2/2H have extensive sequence polymorphism without losing vitronectin-binding capacity promoting a general evasion of the host immune system. PMID:23474333

  15. Genetic Diversity among Strains of Moraxella catarrhalis: Analysis Using Multiple DNA Probes and a Single-Locus PCR-Restriction Fragment Length Polymorphism Method

    PubMed Central

    Walker, Elaine S.; Preston, Robert A.; Post, J. Christopher; Ehrlich, Garth D.; Kalbfleisch, John H.; Klingman, Karin L.

    1998-01-01

    Moraxella (Branhamella) catarrhalis, a causative agent of otitis media, sinusitis, and exacerbation of bronchitis, has acquired widespread ability to produce ?-lactamase and can be nosocomially transmitted. The typing methods used in epidemiological analyses of M. catarrhalis are not optimal for genetic analyses. Two methods, a multiple-locus Southern blot (SB) method and a single-locus PCR-restriction fragment length polymorphism (RFLP) method, were developed and used to assess genetic diversity and potential clinical and geographic relationships in M. catarrhalis. Nine randomly cloned M. catarrhalis DNA fragments were used as probes of SBs containing DNA from 54 geographically and clinically diverse strains. For comparison, a PCR-RFLP method was developed as a quick, inexpensive, and discriminating alternative. A highly variable 3.7-kb genomic region (M46) was cloned and sequenced, and 3.5 kb of the cloned DNA was targeted for PCR amplification. DNAs from the 54 strains were subjected to PCR-RFLP. SB analysis distinguished all strains that had no apparent epidemiological linkage (40 of 54), and PCR-RFLP distinguished fewer strains (21 of 54). Epidemiologically linked strains appeared genetically identical by both methods. PCR-RFLP was compared to pulsed-field gel electrophoresis (PFGE) for 8 of the 54 strains and 23 additional strains. PCR-RFLP distinguished fewer strains than PFGE typing (16 of 31 versus 20 of 31 strains), but PCR-RFLP was more useful for inferring interstrain relatedness. Separate cluster analyses of multilocus SB and single locus PCR-RFLP data showed high genetic diversity within and across geographic locations and clinical presentations. The resultant dendrograms were not entirely concordant, but both methods often gave similar strain clusters at the terminal branches. High genetic diversity, nonconcordance of cluster analyses from different genetic loci, and shared genotypes among epidemiologically linked strains support a hypothesis of high recombination relative to spread of clones. Single-locus PCR-RFLP may be suitable for short-term epidemiological studies, but the SB data demonstrate that greater strain discrimination may be obtained by sampling variation at multiple genomic sites. PMID:9650948

  16. Ligand-bound structures of 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Moraxella catarrhalis reveal a water channel connecting to the active site for the second step of catalysis.

    PubMed

    Dhindwal, Sonali; Priyadarshini, Priyanka; Patil, Dipak N; Tapas, Satya; Kumar, Pramod; Tomar, Shailly; Kumar, Pravindra

    2015-02-01

    KdsC, the third enzyme of the 3-deoxy-D-manno-octulosonic acid (KDO) biosynthetic pathway, catalyzes a substrate-specific reaction to hydrolyze 3-deoxy-D-manno-octulosonate 8-phosphate to generate a molecule of KDO and phosphate. KdsC is a phosphatase that belongs to the C0 subfamily of the HAD superfamily. To understand the molecular basis for the substrate specificity of this tetrameric enzyme, the crystal structures of KdsC from Moraxella catarrhalis (Mc-KdsC) with several combinations of ligands, namely metal ion, citrate and products, were determined. Various transition states of the enzyme have been captured in these crystal forms. The ligand-free and ligand-bound crystal forms reveal that the binding of ligands does not cause any specific conformational changes in the active site. However, the electron-density maps clearly showed that the conformation of KDO as a substrate is different from the conformation adopted by KDO when it binds as a cleaved product. Furthermore, structural evidence for the existence of an intersubunit tunnel has been reported for the first time in the C0 subfamily of enzymes. A role for this tunnel in transferring water molecules from the interior of the tetrameric structure to the active-site cleft has been proposed. At the active site, water molecules are required for the formation of a water bridge that participates as a proton shuttle during the second step of the two-step phosphoryl-transfer reaction. In addition, as the KDO biosynthesis pathway is a potential antibacterial target, pharmacophore-based virtual screening was employed to identify inhibitor molecules for the Mc-KdsC enzyme. PMID:25664734

  17. Carcinoembryonic antigen (CEA)-related cell adhesion molecules are co-expressed in the human lung and their expression can be modulated in bronchial epithelial cells by non-typable Haemophilus influenzae, Moraxella catarrhalis, TLR3, and type I and II interferons

    PubMed Central

    2013-01-01

    Background The carcinoembryonic antigen (CEA)-related cell adhesion molecules CEACAM1 (BGP, CD66a), CEACAM5 (CEA, CD66e) and CEACAM6 (NCA, CD66c) are expressed in human lung. They play a role in innate and adaptive immunity and are targets for various bacterial and viral adhesins. Two pathogens that colonize the normally sterile lower respiratory tract in patients with chronic obstructive pulmonary disease (COPD) are non-typable Haemophilus influenzae (NTHI) and Moraxella catarrhalis. Both pathogens bind to CEACAMs and elicit a variety of cellular reactions, including bacterial internalization, cell adhesion and apoptosis. Methods To analyze the (co-) expression of CEACAM1, CEACAM5 and CEACAM6 in different lung tissues with respect to COPD, smoking status and granulocyte infiltration, immunohistochemically stained paraffin sections of 19 donors were studied. To address short-term effects of cigarette smoke and acute inflammation, transcriptional regulation of CEACAM5, CEACAM6 and different CEACAM1 isoforms by cigarette smoke extract, interferons, Toll-like receptor agonists, and bacteria was tested in normal human bronchial epithelial (NHBE) cells by quantitative PCR. Corresponding CEACAM protein levels were determined by flow cytometry. Results Immunohistochemical analysis of lung sections showed the most frequent and intense staining for CEACAM1, CEACAM5 and CEACAM6 in bronchial and alveolar epithelium, but revealed no significant differences in connection with COPD, smoking status and granulocyte infiltration. In NHBE cells, mRNA expression of CEACAM1 isoforms CEACAM1-4L, CEACAM1-4S, CEACAM1-3L and CEACAM1-3S were up-regulated by interferons alpha, beta and gamma, as well as the TLR3 agonist polyinosinic:polycytidylic acid (poly I:C). Interferon-gamma also increased CEACAM5 expression. These results were confirmed on protein level by FACS analysis. Importantly, also NTHI and M. catarrhalis increased CEACAM1 mRNA levels. This effect was independent of the ability to bind to CEACAM1. The expression of CEACAM6 was not affected by any treatment or bacterial infection. Conclusions While we did not find a direct correlation between CEACAM1 expression and COPD, the COPD-associated bacteria NTHi and M. catarrhalis were able to increase the expression of their own receptor on host cells. Further, the data suggest a role for CEACAM1 and CEACAM5 in the phenomenon of increased host susceptibility to bacterial infection upon viral challenge in the human respiratory tract. PMID:23941132

  18. Increase in bronchopulmonary infection due to branhamella catarrhalis.

    PubMed Central

    McLeod, D T; Ahmad, F; Capewell, S; Croughan, M J; Calder, M A; Seaton, A

    1986-01-01

    In a six month prospective study during the winter Branhamella catarrhalis was isolated from the sputum of 63 patients with symptoms of bronchopulmonary infection: 49 isolates were in pure culture and 14 were with another pathogen, Haemophilus influenzae being the commonest (found with 10 of the 14 B catarrhalis isolates). Of 36 patients infected in the community, 26 required admission to hospital. The remaining 27 patients were infected while in hospital. Forty four of the 63 isolates produced beta lactamase; 26 of these had been acquired in the community. As a result 29 patients were treated inappropriately with ampicillin and did not respond to this treatment. beta Lactamase produced by B catarrhalis may also protect other pathogens normally susceptible to beta lactam antibiotics. Most patients had chronic lung diseases or lung cancer, but three otherwise healthy patients who did not smoke developed bronchitis. B catarrhalis contributed to the death of five patients. A survey of the antibiotic prescribing habits of the referring general practitioners together with the sensitivity results of B catarrhalis suggest that changes in antibiotic prescribing habits in the community may be responsible for the increase in B catarrhalis infection. PMID:3084017

  19. Phylogenetic analysis and genetic diversity of 3' region of rtxA gene from geographically diverse strains of Moraxella bovis, Moraxella bovoculi and Moraxella ovis.

    PubMed

    Farias, Luana D'Avila; Maboni, Grazieli; Matter, Letícia Beatriz; Scherer, Charles Fernando Capinos; Libardoni, Felipe; Vargas, Agueda Castagna de

    2015-08-01

    The cytotoxin A (MbxA) is one of the main virulence factors of Moraxella bovis involved in the pathogenesis of infectious bovine keratoconjunctivitis (IBK). Moraxella ovis and Moraxella bovoculi, suspected to be associated with infectious keratitis in sheep and cattle respectively, also have a gene that encodes the cytotoxin A (movA and mbvA, respectively). The aim of this study was to determine the molecular sequence of the 3' region of the cytotoxin gene of Moraxella spp. strains isolated from clinical cases to establish phylogenetic and evolutionary comparisons. PCR amplification, nucleotide sequencing (nt) and amino acid (aa) sequence prediction were performed, followed by the sequences comparison, identity level calculation and selective pressure analysis. The phylogenetic reconstruction based on nt and aa sequences clearly differentiate M. bovis (n=15), M. bovoculi (n=11) and M. ovis (n=7) and their respective reference strains. An alignment of 843nt revealed high similarity within bacterial species (MbxA=99.9% nt and aa; MbvA=99.3% nt and 98.8% aa; MovA=99.5% nt and 99.3% aa). The similarity of partial sequences (nt 1807-2649) of MbxA in relation to MbvA and MovA ranged from 76.3 to 78.5%; similarity between MbvA and MovA ranged from 95.7 to 97.5%. A negative selection on mbvA and movA sequences was revealed by the molecular evolution analysis. The phylogenetic analysis of movA and mbvA allowed different strains of Moraxella spp. to be grouped according to the period of isolation. Sequence analysis of cytotoxin may provide insights into genetic and evolutionary relationships and into the genetic/molecular basis of Moraxella spp. PMID:26036790

  20. Bovine Infectious Keratoconjunctivitis: Serological Aspects of Moraxella bovis Infection

    PubMed Central

    Pugh, G. W.; Hughes, D. E.; McDonald, T. J.

    1971-01-01

    A modification of a gel diffusion precipitin test (GDPT) was used to detect antibodies for Moraxella bovis (M. bovis) in the sera of cattle affected with bovine infectious keratoconjunctivitis (BIK). The test was also used for the detection of sequential antibody development in cattle vaccinated with cultures of M. bovis. Also, strains of M. bovis isolated from cattle herds affected with BIK were characterized serologically as a part of an identification scheme using the test. A comparison of the antigenic properties of various strains of M. bovis and M. bovis-like organisms was conducted using the test. The results indicated that there might be antigenic relationships between M. bovisand M. bovis-like organisms such as Moraxella liquefaciens, Moraxella nonliquefaciens, an unidentified hemolytic diplococcus, Mima polymorpha, Mima polymorpha var. oxidans and Herellea vaginicola The authors suggest that the GDPT can be used for serological studies of BIK, and the identification and antigenic analysis of M. bovis. They indicate, however, that a more definitive study is needed to evaluate the reliability of the test for quantitative work. ImagesFig. 1.Fig. 2. PMID:4253466

  1. Moraxella Species Are Primarily Responsible for Generating Malodor in Laundry

    PubMed Central

    Mitani, Asako; Niwano, Yu; Takeuchi, Kohei; Tanaka, Atsushi; Yamaguchi, Noriko; Kawamura, Yoshiaki; Hitomi, Jun

    2012-01-01

    Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a “wet-and-dirty-dustcloth-like malodor” or an “acidic or sweaty odor.” In this study, we isolated the major microorganisms associated with such a malodor, the major component of which has been identified as 4-methyl-3-hexenoic acid (4M3H). The isolates were identified as Moraxella osloensis by morphological observation and biochemical and phylogenetic tree analyses. M. osloensis has the potential to generate 4M3H in laundry. The bacterium is known to cause opportunistic infections but has never been known to generate a malodor in clothes. We found that M. osloensis exists at a high frequency in various living environments, particularly in laundry in Japan. The bacterium showed a high tolerance to desiccation and UV light irradiation, providing one of the possible reasons why they survive in laundry during and even after drying. PMID:22367080

  2. Relatedness of cytotoxins from geographically diverse isolates of Moraxella bovis.

    PubMed

    Angelos, John A; Ball, Louise M

    2007-10-01

    To determine whether amino acid sequence variation exists in the Moraxella bovis (M. bovis) cytotoxin (MbxA) from geographically diverse M. bovis isolated in the United States, mbxA was amplified and sequenced. The MbxA deduced amino acid sequence from M. bovis originally isolated in California, Washington, North Carolina, and Georgia, as well as reference strains of M. bovis isolated at the National Animal Disease Laboratory, Ames, IA, USA, all encoded a nearly identical 927 amino acid protein. MbxA from two of the four California isolates (SFS 9a and SFS 100a) differed from all other isolates at two sites at which the polar amino acids glutamine (position 666) and asparagine (position 823) were replaced by ionized amino acids glutamic acid and aspartic acid, respectively. Rabbit antiserum to the expressed carboxy terminus (amino acids 590-927) of MbxA from M. bovis (Tifton I) neutralized the hemolytic activity of SFS 9a and SFS 100a. The M. bovis cytotoxin appears to be conserved amongst geographically diverse isolates of M. bovis from the USA. Antiserum against the carboxy terminus of MbxA common to the majority of isolates neutralized the hemolytic activity of two strains with a divergent MbxA deduced amino acid sequence. Vaccines against IBK that incorporate MbxA as antigen may offer protection against geographically diverse strains of M. bovis. PMID:17540518

  3. Moraxella species are primarily responsible for generating malodor in laundry.

    PubMed

    Kubota, Hiromi; Mitani, Asako; Niwano, Yu; Takeuchi, Kohei; Tanaka, Atsushi; Yamaguchi, Noriko; Kawamura, Yoshiaki; Hitomi, Jun

    2012-05-01

    Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a "wet-and-dirty-dustcloth-like malodor" or an "acidic or sweaty odor." In this study, we isolated the major microorganisms associated with such a malodor, the major component of which has been identified as 4-methyl-3-hexenoic acid (4M3H). The isolates were identified as Moraxella osloensis by morphological observation and biochemical and phylogenetic tree analyses. M. osloensis has the potential to generate 4M3H in laundry. The bacterium is known to cause opportunistic infections but has never been known to generate a malodor in clothes. We found that M. osloensis exists at a high frequency in various living environments, particularly in laundry in Japan. The bacterium showed a high tolerance to desiccation and UV light irradiation, providing one of the possible reasons why they survive in laundry during and even after drying. PMID:22367080

  4. Analytica Chimica Acta 470 (2002) 7986 Microbial biosensor for p-nitrophenol using Moraxella sp.

    E-print Network

    Chen, Wilfred

    2002-01-01

    monitoring [3]. Biosensor technology based on enzyme or micro- organism is well suited for rapid, cost-Nitrophenol; Microbial biosensor; Moraxella sp.; Respiration 1. Introduction p-Nitrophenol (PNP), because of its toxicity of an accidental release during these industrial activities. Current EPA recommended analytical technique for PNP

  5. The metabolism of benzoate by Moraxella species through anaerobic nitrate respiration. Evidence for a reductive pathway.

    PubMed Central

    Williams, R J; Evans, W C

    1975-01-01

    Moraxella sp. isolated from soil grows anaerobically on benzoate by nitrate respiration; nitrate or nitrite are obligatory electron acceptors, being reduced to molecular N2 during the catabolism of the substrate. This bacterium also grows aerobically on benzoate. 2. Aerobically, benzoate is metabolized by ortho cleavage of catechol followed by the beta-oxoadipate pathway. 3. Cells of Moraxella grown anaerobically on benzoate are devoid of ortho and meta cleavage enzymes; cyclohexanecarboxylate and 2-hydroxycyclohexanecarboxylate were detected in the anaerobic culture fluid. 4. [ring-U-14C]Benzoate, incubated anaerobically with cells in nitrate-phosphate buffer, gave rise to labelled 2-hydroxycyclohexanecarboxylate and adipate. When [carboxy-14C]benzoate was used, 2-hydroxycyclohexanecarboxylate was radioactive but the adipate was not labelled. A decarboxylation reaction intervenes at some stage between these two metabolites. 5. The anaerobic metabolism of benzoate by Moraxella sp. through nitrate respiration takes place by the reductive pathway (Dutton & Evans, 1969). Hydrogenation of the aromatic ring probably occurs via cyclohexa-2,5-dienecarboxylate and cyclohex-1-enecarboxylate to give cyclohexanecarboxylate. The biochemistry of this reductive process remains unclear. 6. CoA thiol esterification of cyclohexanecarboxylate followed by beta-oxidation via the unsaturated and hydroxy esters, would afford 2-oxocyclohexanecarboxylate. Subsequent events in the Moraxella culture differ from those occurring with Rhodopseudomonas palustris; decarboxylation precedes hydrolytic cleavage of the alicyclic ring to produce adipate in the former, whereas in the latter the keto ester undergoes direct hydrolytic fission to pimelate. PMID:1156389

  6. Septic Arthritis Due to Moraxella osloensis in a Rhesus Macaque (Macaca mulatta)

    PubMed Central

    Wren, Melissa A; Caskey, John R; Liu, David X; Embers, Monica E

    2013-01-01

    A 5.5-y-old Chinese-origin female rhesus macaque (Macaca mulatta) presented for bilateral hindlimb lameness. The primate had been group-reared in an SPF breeding colony and was seronegative for Macacine herpesvirus 1, SIV, simian retrovirus type D, and simian T-lymphotropic virus. The macaque's previous medical history included multiple occasions of swelling in the left tarsus, and trauma to the right arm and bilateral hands. In addition, the macaque had experienced osteomyelitis of the left distal tibia and rupture of the right cranial cruciate ligament that had been surgically repaired. Abnormal physical examination findings on presentation included a thin body condition, mild dehydration, and bilaterally swollen stifles that were warm to the touch, with the right stifle more severely affected. Mild instability in the left stifle was noted, and decreased range of motion and muscle atrophy were present bilaterally. Hematologic findings included marked neutrophilia and lymphopenia and moderate anemia. Arthrocentesis and culture of joint fluid revealed Moraxella-like organisms. Treatment with enrofloxacin was initiated empirically and subsequently switched to cephalexin, which over time alleviated the joint swelling and inflammation. Definitive diagnosis of Moraxella osloensis septic arthritis was made through isolation of the organism and sequencing of the 16S rDNA region. To our knowledge, this report is the first description of Moraxella osloensis septic arthritis in a rhesus macaque. PMID:24326229

  7. Influence of Moraxella sp. colonization on the kidney proteome of farmed gilthead sea breams (Sparus aurata, L.)

    PubMed Central

    2010-01-01

    Background Currently, presence of Moraxella sp. in internal organs of fish is not considered detrimental for fish farming. However, bacterial colonization of internal organs can affect fish wellness and decrease growth rate, stress resistance, and immune response. Recently, there have been reports by farmers concerning slow growth, poor feed conversion, and low average weight increase of fish farmed in offshore floating sea cages, often associated with internal organ colonization by Moraxella sp. Therefore, presence of these opportunistic bacteria deserves further investigations for elucidating incidence and impact on fish metabolism. Results A total of 960 gilthead sea breams (Sparus aurata, L.), collected along 17 months from four offshore sea cage plants and two natural lagoons in Sardinia, were subjected to routine microbiological examination of internal organs throughout the production cycle. Thirteen subjects (1.35%) were found positive for Moraxella sp. in the kidney (7), brain (3), eye (1), spleen (1), and perivisceral fat (1). In order to investigate the influence of Moraxella sp. colonization, positive and negative kidney samples were subjected to a differential proteomics study by means of 2-D PAGE and mass spectrometry. Interestingly, Moraxella sp. infected kidneys displayed a concerted upregulation of several mitochondrial enzymes compared to negative tissues, reinforcing previous observations following lipopolysaccharide (LPS) challenge in fish. Conclusions Presence of Moraxella sp. in farmed sea bream kidney is able to induce proteome alterations similar to those described following LPS challenge in other fish species. This study revealed that Moraxella sp. might be causing metabolic alterations in fish, and provided indications on proteins that could be investigated as markers of infection by Gram-negative bacteria within farming plants. PMID:20939867

  8. Factors affecting inactivation of Moraxella-Acinetobacter cells in an irradiation process.

    PubMed Central

    Firstenberg-Eden, R; Rowley, D B; Shattuck, G E

    1980-01-01

    The effect of various stages of the irradiation processing of beef on the injury and inactivation of radiation-resistant Moraxella-Acinetobactor cells was studied. Moraxella-Acinetobacter cells were more resistant to heat inactivation and injury when heated in meat with salts (0.75% NaCl and 0.375% sodium tripolyphosphate) then in meat without salts. These salts had no effect on radiation resistance. Both radiation- and heat-injured cells were unable to form colonies at 30 degrees C in plate count agar containing 0.8% NaCl. Neither unstressed nor heat-stressed cells were able to multiply in minced beef incubated at 30 degrees C for 12 h. Only after the beef was diluted 1:10 with peptone water were the heat-injured cells able to repair and eventually multiply. Heated cells were more sensitive to radiation inactivation and injury than unheated cells. After repair, the cells regained their resistance to both NaCl and irradiation. Freezing and storage at -40 degrees C for 14 days had only a slight effect on either unstressed or heat-stressed cells. PMID:7191692

  9. Iron repressible outer membrane proteins of Moraxella bovis and demonstration of siderophore-like activity.

    PubMed

    Fenwick, B; Rider, M; Liang, J; Brightman, A

    1996-02-01

    Moraxella bovis (strain Epp 63), grown in RPMI 1640 medium supplemented with desferrioxamine mesylate (0.05 mg/ml) resulted in cell free culture supernatants with an increased chromeazurol-S response indicating the presence of high affinity iron binding ligand(s). Supernatants of cultures where growth occurred in tryptic soy broth, RPMI 1640, or RPMI 1640-desferrioxamine supplemented with ferrous sulfate (10 micrograms/ml) were negative on the chromeazurol-S test. Growth of M. bovis in RPMI 1640 or RPMI 1640-desferrioxamine medium induced the expression of previously unrecognized outer membrane proteins whose expression was repressed when the medium was supplemented with iron and which were not produced when growth occurred in tryptic soy broth. PMID:9054127

  10. Isolation, purification and spectrometric analysis of PSP toxins from moraxella sp., a bacterium associated with a toxic dinoflagellate

    SciTech Connect

    Boyce, S.D.; Doucette, G.J.

    1994-12-31

    Paralytic shellfish poisoning (PSP) is a seafood intoxication syndrome caused by the injestion of shellfish contaminated with toxins produced by algae known as dinoflagellates. The PSP toxins, saxitoxin and its derivatives, act to block voltage-dependent sodium channels and can cause paralysis and even death at higher doses. It is well documented that bacteria coexist with many harmful or toxic algal species, though the exact nature of the association in relation to toxin production is unknown. Recently, the bacterium Moraxella sp. was isolated from the PSP toxin producing dinoflagellate Alexandrium tamarense. Through HPLC analysis and saxitoxin receptor binding assays performed on crude bacterial extracts, it appears that Moraxella sp. is capable of producing saxitoxin and several of its derivatives. However, physical confirmation (e.g. mass spectrometry) of these results is still needed.

  11. Pathway for Biodegradation of p-Nitrophenol in a Moraxella sp

    PubMed Central

    Spain, Jim C.; Gibson, David T.

    1991-01-01

    A Moraxella strain grew on p-nitrophenol with stoichiometric release of nitrite. During induction of the enzymes for growth on p-nitrophenol, traces of hydroquinone accumulated in the medium. In the presence of 2,2?-dipyridyl, p-nitrophenol was converted stoichiometrically to hydroquinone. Particulate enzymes catalyzed the conversion of p-nitrophenol to hydroquinone in the presence of NADPH and oxygen. Soluble enzymes catalyzed the conversion of hydroquinone to ?-hydroxymuconic semialdehyde, which was identified by high-performance liquid chromatography (HPLC)-mass spectroscopy. Upon addition of catalytic amounts of NAD+, ?-hydroxymuconic semialdehyde was converted to ?-ketoadipic acid. In the presence of pyruvate and lactic dehydrogenase, substrate amounts of NAD were required and ?-hydroxymuconic semialdehyde was converted to maleylacetic acid, which was identified by HPLC-mass spectroscopy. Similar results were obtained when the reaction was carried out in the presence of potassium ferricyanide. Extracts prepared from p-nitrophenol-growth cells also contained an enzyme that catalyzed the oxidation of 1,2,4-benzenetriol to maleylacetic acid. The enzyme responsible for the oxidation of 1,2,4-benzenetriol was separated from the enzyme responsible for hydroquinone oxidation by DEAE-cellulose chromatography. The results indicate that the pathway for biodegradation of p-nitrophenol involves the initial removal of the nitro group as nitrite and formation of hydroquinone. 1,4-Benzoquinone, a likely intermediate in the initial reaction, was not detected. Hydroquinone is converted to ?-ketoadipic acid via ?-hydroxymuconic semialdehyde and maleylacetic acid. PMID:16348446

  12. Pilin-gene phase variation of Moraxella bovis is caused by an inversion of the pilin genes.

    PubMed Central

    Marrs, C F; Ruehl, W W; Schoolnik, G K; Falkow, S

    1988-01-01

    Moraxella bovis Epp63 can express either of two different pilin proteins, called alpha and beta. We have previously cloned and sequenced the beta-pilin gene and now report that DNAs isolated from bacteria expressing alpha pilin have hybridization patterns consistently different from those of bacteria expressing beta pilin. The phase variation between alpha- and beta-pilin gene expression appears to be associated with an inversion of about 2 kilobases of DNA, whose endpoints occur within the coding region of the expressed pilin gene. Comparisons of the beta-pilin gene sequence with those of well-studied bacterial inversion systems revealed a stretch of 58% sequence similarity (21 of 36 base pairs) between the left inverted repeat of the Salmonella typhimurium flagellar hin control region and the amino-terminal portion of the beta-pilin gene. Images PMID:2898471

  13. Identification, cloning, and sequencing of piv, a new gene involved in inverting the pilin genes of Moraxella lacunata.

    PubMed Central

    Marrs, C F; Rozsa, F W; Hackel, M; Stevens, S P; Glasgow, A C

    1990-01-01

    Moraxella lacunata is a bacterium that is a causative agent of human conjunctivitis and keratitis. We have previously cloned the Q and I pilin (formerly called beta and alpha pilin) genes of Moraxella bovis and determined that an inversion of 2 kilobases (kb) of DNA determines which pilin gene is expressed. Using an M. bovis pilin gene as a hybridization probe to screen a lambda ZAP library of M. lacunata DNA, we have isolated a clone that not only contains the entire type 4 pilin gene inversion region of M. lacunata but inverts the 2-kb region on a plasmid subclone (pMxL1) in Escherichia coli. Deletion derivatives of pMxL1 yielded some plasmids that still had the entire inversion region but were phase locked into one or the other of the two potential orientations. Similarly, insertions of a 2-kb streptomycin-resistant element (omega) within some regions outside of the inversion also resulted in phase-locked plasmids. These deletions and insertions thus localize a probable invertase necessary for the inversion event. The region was sequenced, and an open reading frame with over 98% DNA sequence homology to an open reading frame that we previously found in M. bovis and called ORF2 appeared to be a strong candidate for the invertase. This conclusion was confirmed when a plasmid containing the M. bovis ORF2 supplied, in trans, the inversion function missing from one of the M. lacunata phase-locked inversion mutants. We have named these putative invertase genes piv(ml) (pilin inversion of M. lacunata) and piv(mb) (pilin inversion of M. bovis). Despite previously noted sequence similarities between the M. bovis sites of inversion and those of the Hin family of invertible segments and a 60-base-pair region within the inversion with 50% sequence similarity to the cin recombinational enhancer, there is no significant sequence similarity of the Piv invertases to the Hin family of invertases. Images PMID:1973927

  14. Amino acid sequence homology between Piv, an essential protein in site-specific DNA inversion in Moraxella lacunata, and transposases of an unusual family of insertion elements.

    PubMed Central

    Lenich, A G; Glasgow, A C

    1994-01-01

    Deletion analysis of the subcloned DNA inversion region of Moraxella lacunata indicates that Piv is the only M. lacunata-encoded factor required for site-specific inversion of the tfpQ/tfpI pilin segment. The predicted amino acid sequence of Piv shows significant homology solely with the transposases/integrases of a family of insertion sequence elements, suggesting that Piv is a novel site-specific recombinase. Images PMID:8021196

  15. Assessment of Bdellovibrio bacteriovorus 109J killing of Moraxella bovis in an in vitro model of infectious bovine keratoconjunctivitis.

    PubMed

    Boileau, Mélanie J; Clinkenbeard, Kenneth D; Iandolo, John J

    2011-10-01

    The objective of this study was to determine the potential of Bdellovibrio bacteriovorus 109J as an alternative non-chemotherapeutic treatment of infectious bovine keratoconjunctivitis (IBK). To accomplish this, various parameters of B. bacteriovorus predation of Moraxella bovis were determined in vitro. Initial passage of B. bacteriovorus using M. bovis as prey required 10 d for active cultures to develop compared with 2 d for culture on normal Escherichia coli prey; however by the 5th passage, time to active predatory morphology was reduced to 2 d. This high passage B. bacteriovorus culture [1 × 10(10) plaque forming units (PFU)/mL] killed 76% of M. bovis [1 × 10(7) colony forming units (CFU)/mL] present in suspension broth in a 4 h assay. The minimal level of M. bovis supporting B. bacteriovorus predation was 1 × 10(4) CFU/mL. To assess the ability of B. bacteriovorus to kill M. bovis on an epithelial surface mimicking IBK, an in vitro assay with Madin-Darby bovine kidney (MDBK) cells inoculated with 4 × 10(7) CFU/mL M. bovis was used. Treatment with a B. bacteriovorus suspension (1.6 × 10(11) PFU/mL) decreased adherence of M. bovis to MDBK cells by 6-fold at 12 h of treatment, as well as decreased the number of unattached M. bovis cells by 1.4-fold. This study demonstrates that B. bacteriovorus has potential as an effective biological control of M. bovis at levels likely present in IBK-infected corneal epithelia and ocular secretions. PMID:22468026

  16. Ocular immune responses in steers following intranasal vaccination with recombinant Moraxella bovis cytotoxin adjuvanted with polyacrylic acid.

    PubMed

    Angelos, John A; Edman, Judy M; Chigerwe, Munashe

    2014-02-01

    Infectious bovine keratoconjunctivitis (IBK) caused by Moraxella bovis is the most common eye disease of cattle. The pathogenesis of M. bovis requires the expression of pili that enable the organism to attach to the ocular surface and an RTX (repeats in the structural toxin) toxin (cytotoxin or hemolysin), which is cytotoxic to corneal epithelial cells. In this pilot study, ocular mucosal immune responses of steers were measured following intranasal (i.n.) vaccination with a recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid. Beef steers were vaccinated with either 500 ?g (n = 3) or 200 ?g (n = 3) of recombinant M. bovis cytotoxin plus adjuvant. Control group steers (n = 2) were vaccinated with adjuvant alone, and all steers were given a booster on day 21. Antigen-specific tear IgA and tear IgG, tear cytotoxin-neutralizing antibody responses, and serum cytotoxin-neutralizing antibody responses were determined in samples collected prevaccination and on days 14, 28, 42, and 55. Changes in tear antigen-specific IgA levels from day 0 to days 28, 42, and 55 were significantly different between groups; however, in post hoc comparisons between individual group pairs at the tested time points, the differences were not significant. Our results suggest that i.n. vaccination of cattle with recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid effects changes in ocular antigen-specific IgA concentrations. The use of intranasally administered recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid could provide an alternative to parenteral vaccination of cattle for immunoprophylaxis against IBK. PMID:24334685

  17. Ocular Immune Responses in Steers following Intranasal Vaccination with Recombinant Moraxella bovis Cytotoxin Adjuvanted with Polyacrylic Acid

    PubMed Central

    Edman, Judy M.; Chigerwe, Munashe

    2014-01-01

    Infectious bovine keratoconjunctivitis (IBK) caused by Moraxella bovis is the most common eye disease of cattle. The pathogenesis of M. bovis requires the expression of pili that enable the organism to attach to the ocular surface and an RTX (repeats in the structural toxin) toxin (cytotoxin or hemolysin), which is cytotoxic to corneal epithelial cells. In this pilot study, ocular mucosal immune responses of steers were measured following intranasal (i.n.) vaccination with a recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid. Beef steers were vaccinated with either 500 ?g (n = 3) or 200 ?g (n = 3) of recombinant M. bovis cytotoxin plus adjuvant. Control group steers (n = 2) were vaccinated with adjuvant alone, and all steers were given a booster on day 21. Antigen-specific tear IgA and tear IgG, tear cytotoxin-neutralizing antibody responses, and serum cytotoxin-neutralizing antibody responses were determined in samples collected prevaccination and on days 14, 28, 42, and 55. Changes in tear antigen-specific IgA levels from day 0 to days 28, 42, and 55 were significantly different between groups; however, in post hoc comparisons between individual group pairs at the tested time points, the differences were not significant. Our results suggest that i.n. vaccination of cattle with recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid effects changes in ocular antigen-specific IgA concentrations. The use of intranasally administered recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid could provide an alternative to parenteral vaccination of cattle for immunoprophylaxis against IBK. PMID:24334685

  18. Bacterial differentiation within Moraxella bovis colonies growing at the interface of the agar medium with the Petri dish.

    PubMed

    McMichael, J C

    1992-12-01

    Moraxella bovis was found to colonize the interface between agar and the polystyrene Petri dish, producing circular colonies when the inoculum was stabbed at a single point. The bacteria occurred in a thin layer of nearly uniform thickness, and colonial expansion occurred in at least two temporal phases. In the first phase, the radial colonial expansion was slow and non-linear. In the second phase, the radial expansion was linear. The interfacial colonies possessed three characteristic concentric growth zones. At the periphery was a narrow ring zone that enclosed another wider ring zone, which, in turn, surrounded a central circular zone. Different bacterial phase variants were recovered from these zones. The two outer ring zones yielded bacteria that formed agar surface colonies of spreading-corroding morphology, while cells from the innermost zone always yielded colonies with a different morphology. The uniform thickness of the colonies implied that replication was restricted to the outermost ring, and that the bacteria within the inner ring and inner circle had entered a quiescent state. The inner ring appeared to represent the lag in time needed for the replicative form to differentiate into the quiescent form. A different kind of variant was associated with wedge-shaped sectors within the colonies. The greatest number of these clonal variants appeared shortly after inoculation and their frequency decreased after the onset of linear growth. The period of slowest colonization coincided with highest frequency of clonal variant expression. It is proposed that the proliferative rate of the parental bacterial population exerted selective pressure on the expression of new clonal variants. PMID:1362585

  19. Changes in nasopharyngeal carriage of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis among healthy children attending a day-care centre before and after official financial support for the 7-valent pneumococcal conjugate vaccine and H. influenzae type b vaccine in Japan.

    PubMed

    Oikawa, Junko; Ishiwada, Naruhiko; Takahashi, Yoshiko; Hishiki, Haruka; Nagasawa, Koo; Takahashi, Sachiko; Watanabe, Masaharu; Chang, Bin; Kohno, Yoichi

    2014-02-01

    The 7-valent pneumococcal conjugate vaccine (PCV7) and Haemophilus influenzae type b (Hib) vaccine reduce nasopharyngeal carriage of vaccine-type bacteria, which may in turn influence the presence of other nasopharyngeal bacterial pathogens. To investigate this possibility, nasopharyngeal carriage of potential pathogens was examined before and after official financial support was provided to offer the PCV7 and Hib vaccines in healthy children attending a day care centre in Japan during 2011-2012. Despite a virtual disappearance of PCV7 serotypes over time, the overall pneumococcal carriage rate remained unchanged. Although others have reported an increase in PCV13 serotypes following PCV7 vaccination, only non-PCV13 serotypes were observed to have increased in this study. The majority of H. influenzae isolates were non-typeable and Hib was not found. Our data identified an unexpected pattern of pneumococcal serotype replacement following PCV7. Continuous monitoring of pneumococcal carriage is important for decisions regarding the future of national vaccination policy in Japan. PMID:24582389

  20. Antimicrobial Action of Nitens® Mouthwash (Cetyltrimethylammonium Naproxenate) on Multiple Isolates of Pharyngeal Microbes: A Controlled Study against Chlorhexidine, Benzydamine, Hexetidine, Amoxicillin, Amoxicillin-Clavulanate, Clarithromycin, and Cefaclor

    Microsoft Academic Search

    A. P. Pilloni; G. Buttini; D. Giannarelli; B. Giordano; M. R. Iovene; F. Montella; R. di Salvo; R. Colantuono; G. Lalli; M. A. Tufano

    2002-01-01

    Background: Acute oropharyngeal and respiratory tract infections are due to a wide spectrum of microorganisms. The aim of this study was to compare and evaluate the in vitro activity of four antiseptics (cetyltrimethylammonium naproxenate, chlorhexidine, benzydamine, hexetidine) to four antibiotics (amoxicillin, amoxicillin-clavulanate, clarithromycin, cefaclor) on strains of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pyogenes and Streptococcus pneumoniae. Methods: Susceptibility tests were

  1. Effects of antibiotic treatment in the subset of common-cold patients who have bacteria in nasopharyngeal secretions

    Microsoft Academic Search

    L Kaiser; D Lew; B Hirschel; R Auckenthaler; A Morabia; A Heald; J Voegli; H Stalder; P Benedict; F Terrier; W Wunderli; L Matter; D Germann

    1996-01-01

    SummaryBackground Upper-respiratory-tract infection is one of the main causes of overuse of antibiotics. We have found previously that bacteria such as Haemophilus influenzae, Moraxella catarrhalis, and Streptococcus pneumoniae can be isolated from the nasopharyngeal secretions of a substantial proportion of adults with upper-respiratory-tract infections. We have assessed the efficacy of co-amoxiclav in patients with common colds but no clinical signs

  2. Use of an Oligonucleotide Array for Laboratory Diagnosis of Bacteria Responsible for Acute Upper Respiratory Infections

    Microsoft Academic Search

    Stina B. Roth; Jari Jalava; Olli Ruuskanen; Aino Ruohola; Simo Nikkari

    2004-01-01

    We developed a diagnostic array of oligonucleotide probes targeting species-specific variable regions of the genes encoding topoisomerases GyrB and ParE of respiratory bacterial pathogens. Suitable broad-range primer sequences were designed based on alignment of gyrB\\/parE sequences from nine different bacterial species. These species included Corynebacterium diphtheriae, Fusobacterium necrophorum, Haemophilus influen- zae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus

  3. Changes in adherence of respiratory pathogens to HEp-2 cells induced by subinhibitory concentrations of sparfloxacin, ciprofloxacin, and trimethoprim.

    PubMed Central

    Visser, M R; Beumer, H; Hoepelman, A I; Rozenberg-Arska, M; Verhoef, J

    1993-01-01

    Preincubation with subinhibitory concentrations of sparfloxacin, ciprofloxacin, and trimethoprim decreased the adherence of the respiratory pathogens Klebsiella pneumoniae, Haemophilus influenzae, and Moraxella (Branhamella) catarrhalis to human larynx carcinoma HEp-2 cells. Subinhibitory concentrations of sparfloxacin did not change the adherence of Pseudomonas aeruginosa or Streptococcus pneumoniae 15.62, but adhesion of S. pneumoniae 15.42 was significantly enhanced by subinhibitory antimicrobial concentrations. PMID:8388203

  4. Portable exhausters POR-004 SKID B, POR-005 SKID C, POR-006 SKID D storage plan

    SciTech Connect

    Nelson, O.D.

    1997-09-04

    This document provides a storage plan for portable exhausters POR-004 SKID B, POR-005 SKID C, AND POR-006 SKID D. The exhausters will be stored until they are needed by the TWRS (Tank Waste Remediation Systems) Saltwell Pumping Program. The storage plan provides criteria for portable exhauster storage, periodic inspections during storage, and retrieval from storage.

  5. Evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting Gram-negative rods.

    PubMed

    Bombicino, Karina A; Almuzara, Marisa N; Famiglietti, Angela M R; Vay, Carlos

    2007-01-01

    To evaluate the activity of pyrrolidonyl arylamidase (PYR) for the differentiation and identification of nonfermenting gram negative rods (NFGNR), 293 isolates were tested. A 24 h culture of each test organism was prepared. From this a 108-109 cfu/mL suspension was added to 0.25 mL of sterile physiologic solution. A PYR disk was then added and the test was incubated for 30 minutes at 35-37 degrees C, at environmental atmosphere. Reading was done by adding 1 drop of cinnamaldehyde reagent. Strains of Acinetobacter baumannii, Acinetobacter haemolyticus, Alcaligenes faecalis, Bergeyella zoohelcum, Bordetella bronchiseptica, Bordetella hinzii, Brevundimonas diminuta, Brevundimonas vesicularis, Brucella ovis, Brucella spp., Brucella suis, Burkholderia cepacia complex, Moraxella catarrhalis, Moraxella lacunata, Moraxella nonliquefaciens, Moraxella osloensis, Oligella ureolytica, Pseudomonas alcaligenes, Pseudomonas mendocina, Pseudomonas pseudoalcaligenes, Pseudomonas putida, Pseudomonas stutzeri, Pseudomonas Vb3, Psychrobacter phenylpyruvicus, and Stenotrophomonas maltophilia were PYR negative. On the other hand Achromobacter piechaudii, Achromobacter denitrificans, Achromobacter xylosoxidans, Burkholderia gladioli, Chryseobacterium gleum-indologenes, Comamonas testosroni, Cupriavidus pauculus, Delftia acidovorans, Elizabethkingia meningoseptica, Myroides spp., Ochrobactrum anthropi, Pseudomonas oryzihabitans, Ralstonia pickettii, Rhizobium radiobacter, Shewanella spp., Sphingobacterium multivorum, Sphingobacterium spiritivorum, and Weeksella virosa were PYR positive. Finally, Acinetobacter lwoffii, Pseudomonas aeruginosa, Pseudomonas fluorescens, Roseomonas spp., and Sphingomonas paucimobilis-parapaucimobilis were PYR variable. PYR testing should be considered as a useful tool to facilitate the identification of NFGNR. PMID:16822636

  6. Isolation Of A New Moraxella From A Corneal Abscess

    Microsoft Academic Search

    R. G. A. Sutton; M. F. O'KEEFFE; M. A. Bundock; J. Jeboult; M. P. Tester

    1972-01-01

    The methods and media used were those of Cowan and Steel (1965) with some modifi- cations. Indole, nitrate, methyl-red, Voges-Proskauer and H2S tests were carried out both in the usual media and, because of the poor growth obtained in these media, also in the same media enriched with 0.5 per cent. yeast extract. Carbohydrate fermentation tests were carried out at

  7. Antigenicity and other characteristics of Moraxella (Hemophilus) bovis 

    E-print Network

    Henson, James Bond

    1959-01-01

    or . !Ice 12. ~&'ffoct of' ilil, ;tion nnd 0 . ;iris Mu in ou!louse Path, ?en!city &" !i. Bcvi&si 13. Viability of LyoPhi Lized Cultures of M. Irorvis 1&l. R suits of the Inoculation of t4, Bovie in Rabbits 15. Effect of Variouo Tr. eatments on the P...

  8. Antigenicity and other characteristics of Moraxella (Hemophilus) bovis

    E-print Network

    Henson, James Bond

    1959-01-01

    and photopho'bia accompanied. by ulceration of the affected. corneas. Lacrimal secretions were oollscted by tho introduction of a sterile, dry cotton swab into the con)unctival sac of the affected. eye. The swab was rotated around the con)unctival sac snd... ~ The cotton swabs were then placed 1n tubes containing four ml. of sterile nutrient broth, rota. ted several times and withdrawn. Streptomycin ard penicillin were added to the broth at rates of 100 mg. and 40, 000 units respectively. Following the addition...

  9. Wheeze in Preschool Age Is Associated with Pulmonary Bacterial Infection and Resolves after Antibiotic Therapy

    PubMed Central

    2011-01-01

    Background Neonates with airways colonized by Haemophilus influenzae, Streptococcus pneumoniae or Moraxella catarrhalis are at increased risk for recurrent wheeze which may resemble asthma early in life. It is not clear whether chronic colonization by these pathogens is causative for severe persistent wheeze in some preschool children and whether these children might benefit from antibiotic treatment. We assessed the relevance of bacterial colonization and chronic airway infection in preschool children with severe persistent wheezing and evaluated the outcome of long-time antibiotic treatment on the clinical course in such children. Methodology/Principal Findings Preschool children (n?=?42) with severe persistent wheeze but no symptoms of acute pulmonary infection were investigated by bronchoscopy and bronchoalveolar lavage (BAL). Differential cell counts and microbiological and virological analyses were performed on BAL samples. Patients diagnosed with bacterial infection were treated with antibiotics for 2–16 weeks (n?=?29). A modified ISAAC questionnaire was used for follow-up assessment of children at least 6 months after bronchoscopy. Of the 42 children with severe wheezing, 34 (81%) showed a neutrophilic inflammation and 20 (59%) of this subgroup had elevated bacterial counts (?104 colony forming units per milliliter) suggesting infection. Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis were the most frequently isolated species. After treatment with appropriate antibiotics 92% of patients showed a marked improvement of symptoms upon follow-up examination. Conclusions/Significance Chronic bacterial infections are relevant in a subgroup of preschool children with persistent wheezing and such children benefit significantly from antibiotic therapy. PMID:22140482

  10. Dominance of Haemophilus influenzae in ear discharge from Indigenous Australian children with acute otitis media with tympanic membrane perforation

    PubMed Central

    2013-01-01

    Background Indigenous Australian children living in remote communities experience high rates of acute otitis media with tympanic membrane perforation (AOMwiP). Otitis media in this population is associated with dense nasopharyngeal colonization of three primary otopathogens; Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis. Little is known about the relative abundance of these pathogens during infection. The objective of this study was to estimate the abundance and concordance of otopathogens in ear discharge and paired nasopharyngeal swabs from children with AOMwiP (discharge of not more than 6 weeks’ duration and perforation size <2%). Methods Culture and quantitative PCR (qPCR) estimation of H. influenzae, S. pneumoniae, M. catarrhalis and total bacterial load were performed on paired nasopharyngeal and ear discharge swabs from 55 Indigenous children with AOMwiP aged 3.5 – 45.6 months and resident in remote communities. Results By culture, H. influenzae, S. pneumoniae, and M. catarrhalis were detected in 80%, 84% and 91% of nasopharyngeal swabs, and 49%, 33% and 4% of ear discharge swabs, respectively. Using qPCR, H. influenzae, S. pneumoniae, and M. catarrhalis were detected in 82%, 82%, and 93% of nasopharyngeal swabs, and 89%, 41% and 18% of ear discharge swabs, respectively. Relative abundance of H. influenzae in ear discharge swabs was 0-68% of the total bacterial load (median 2.8%); whereas S. pneumoniae and M. catarrhalis relative abundances were consistently <2% of the total bacterial load. S. pneumoniae and M. catarrhalis abundances were significantly lower in ear discharge compared with nasopharyngeal swabs (p?=?0.001, p?catarrhalis in the nasopharynx did not predict ear discharge prevalence and abundances of these pathogens. PCR was substantially more sensitive than culture for ear discharge, and a necessary adjunct to standard microbiology. Quantitative methods are required to understand species abundance in polymicrobial infections and may be needed to measure accurately the microbiological impact of interventions and to provide a better understanding of clinical failure in these children. PMID:24099576

  11. Polymerase Chain Reaction, Bacteriologic Detection and Antibiogram of Bacteria Isolated from Otitis Media with Effusion in Children, Shiraz, Iran

    PubMed Central

    Shishegar, Mahmood; Faramarzi, Abolhasan; Kazemi, Tayyebe; Bayat, Akbar; Motamedifar, Mohammad

    2011-01-01

    Background: Otitis media with effusion is one of the leading causes of hearing loss in children. Effective treatment of effusion in the middle ear requires appropriate empirical treatment and characterization of responsible pathogens. Objective of the present study was to detect pathogens in clinical samples from patients with otitis media with effusion in our area and to determine the sensitivity profile of isolated organisms to commonly used antibiotics. Methods: Sixty three samples of middle ear effusion were aseptically obtained from 36 children, who had been treated up to at least two weeks before sampling. They were analyzed using standard bacteriological and multiplex polymerase chain reaction (PCR) assays. Antibiotic susceptibility tests were also performed. Results: PCR analysis showed that DNA of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis were present in 60 (95.2%) of the samples. The culture-positive effusion for Streptococcus Pneumoniae, HaemophilusInfluenzae and Moraxella catarrhalis was 34.9%. Almost all isolates of Streptococcus pneumoniaee were sensitive to ciprofloxacin and erythromycin, and none of them was sensitive to co-trimoxazole. None of H. Influenzae isolates was sensitive to erythromycin, cefixim, co-trimoxazole, ampicillin and amoxicillin. None of M. Catarrhalis isolates was sensitive to ceftriaxone, co-trimoxazole, ampicillin and amoxicillin. Conclusion: Compared with other studies using PCR method, the number of H. influenza isolates was in higher in the present study (95.2%). Antibiotic sensitivity profiles of pathogens isolated in this study were different from others. Thus, we can determine empirical antibiotic therapy based on sensitivity profile in our geographic area. PMID:23115412

  12. Viral-bacterial co-infection in Australian Indigenous children with acute otitis media

    PubMed Central

    2011-01-01

    Background Acute otitis media with perforation (AOMwiP) affects 40% of remote Indigenous children during the first 18 months of life. Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis are the primary bacterial pathogens of otitis media and their loads predict clinical ear state. Our hypothesis is that antecedent respiratory viral infection increases bacterial density and progression to perforation. Methods A total of 366 nasopharyngeal swabs from 114 Indigenous children were retrospectively examined. A panel of 17 respiratory viruses was screened by PCR, and densities of S. pneumoniae, H. influenzae and M. catarrhalis were estimated by quantitative real time PCR. Data are reported by clinical ear state. Results M. catarrhalis (96%), H. influenzae (91%), S. pneumoniae (89%) and respiratory viruses (59%) were common; including rhinovirus (HRV) (38%), polyomavirus (HPyV) (14%), adenovirus (HAdV) (13%), bocavirus (HBoV) (8%) and coronavirus (HCoV) (4%). Geometric mean bacterial loads were significantly higher in children with acute otitis media (AOM) compared to children without evidence of otitis media. Children infected with HAdV were 3 times more likely (p < 0.001) to have AOM with or without perforation. Conclusion This study confirms a positive association between nasopharyngeal bacterial load and clinical ear state, exacerbated by respiratory viruses, in Indigenous children. HAdV was independently associated with acute ear states. PMID:21649905

  13. Concurrent Assay for Four Bacterial Species Including Alloiococcus Otitidis in Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report

    PubMed Central

    Ta?tan, Eren; Yücel, Mihriban; Aydo?an, Filiz; Karakoç, Esra; Arslan, Necmi; Kantekin, Yunus; Demirci, Münir

    2012-01-01

    Objectives To detect the prevalences of Alloiococcus otitidis, as well as Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis in children with chronic otitis media with effusion (OME) and to simultaneously investigate the colonization of these bacteria in the nasopharynx and palatine tonsils of these patients. Methods The study included 34 pediatric patients with OME, and 15 controls without OME. In the study group, A. otitidis, H. influenzae, S. pneumoniae, and M. catarrhalis were investigated in the samples obtained from middle ear effusions (MEE), nasopharyngeal swabs (NPS) and tonsillar swabs (TS), using multiplex polymerase chain reaction (PCR) and conventional culture methods. Only the samples obtained from NPS and TS were studied with the same techniques in the control group. Results A. otitidis was isolated only in MEE and only with multiplex PCR method. A. otitidis, S. pneumoniae, M. catarrhalis, H. influenzae were identified in 35%, 8.8%, 8.8%, and 2.9%, respectively, in 34 MEE. A. otitidis was not isolated in NPS or TS of the study and the control groups. Conclusion The prevalence of A.otitidis is high in children with OME and A.otitidis doesn't colonize in the nasopharynx or tonsil. PMID:22737288

  14. Las infecciones emergentes transmitidas por garrapatas

    Microsoft Academic Search

    Ingomar Mutz

    2009-01-01

    ResumenLas infecciones transmitidas por garrapatas aparecen por todo el mundo y son bien conocidas desde hace más de 100 años. Algunas enfermedades transmitidas por garrapatas son muy comunes mientras que otras son extremadamente raras. Las técnicas genéticas moleculares modernas (y la mayor disponibilidad de la reacción en cadena de la polimerasa) han llevado al descubrimiento y la clasificación de nuevos

  15. Por lo menos uno de cada diez

    E-print Network

    Por lo menos uno de cada diez niños en EE. UU. tiene asma. Esto equivale a más de 6 millones de niños. Cada año a causa del asma los niños entre 5 y 17 años de edad pierden 10 millones de días escolares, más que por cualquier otra enfermedad infantil crónica. El asma provoca 3 millones de visitas al

  16. Portable exhauster POR-007/Skid E and POR-008/Skid F storage plan

    SciTech Connect

    Nelson, O.D.

    1998-07-25

    This document provides storage requirements for 1,000 CFM portable exhausters POR-O07/Skid E and POR-008/Skid F. These requirements are presented in three parts: preparation for storage, storage maintenance and testing, and retrieval from storage. The exhauster component identification numbers listed in this document contain the prefix POR-007 or POR-008 depending on which exhauster is being used.

  17. Antimicrobial resistance surveillance of flomoxef in China.

    PubMed

    Cui, Lanqing; Li, Yun; Lv, Yuan; Xue, Feng; Liu, Jian

    2015-05-01

    The aim of this study was to investigate the susceptibility of flomoxef against clinical isolates collected from China and understand the trend of antimicrobial resistance. A total of 2955 pathogenic strains isolated from 18 tertiary hospitals in 18 cities of China over the period from July 2011 to June 2012 were studied. And the susceptibility tests were performed using agar dilution method recommended by CLSI in central laboratory. Flomoxef showed good potency against Enterobacteriaceae with susceptibility rate 85%-100%. The susceptibility rates of flomoxef against Staphylococcus spp. isolates were 63.9%-92.2%; 98.8% of MSSA and 88.2% of MSSE were susceptible to this drug. For other tested bacteria including Moraxella catarrhalis, Haemophilus spp., and Streptococcus spp. (except Viridans group streptococci) flomoxef showed good potency with susceptibility rate more than 95%. All these results strongly suggest that flomoxef is a potent antibacterial agent against major pathogens in China. PMID:25694055

  18. Photodynamic therapy with water-soluble phtalocyanines against bacterial biofilms in teeth root canals

    NASA Astrophysics Data System (ADS)

    Gergova, Raina; Georgieva, Tzvetelina; Angelov, Ivan; Mantareva, Vanya; Valkanov, Serjoga; Mitov, Ivan; Dimitrov, Slavcho

    2012-06-01

    The study presents the PDT with metal phthalocyanines on biofilms grown in root canals of ten representatives of the Gram-positive and the Gram-negative bacterial species and a fungus Candida albicans which cause aqute teeth infections in root canals.. The extracted human single-root teeth infected for 48 h with microorganisms in conditions to form biofilms of the above pathogens were PDT treated. The stage of biofilm formation and PDT effect of the samples of the teeth were determined by the scaning electron microscopy and with standard microbial tests. The PDT treating procedure included 10 min incubation with the respected phthalocyanine and irradiated with 660 nm Diode laser for 10 min. The most strongly antibacterial activity was achieved with zinc(II) phthalocyanine (ZnPc) against Enterococcus faecalis, Staphylococcus aureus and Moraxella catarrhalis. The other Gram-negative bacteria and Candida albicans were 10-100 times more resistant than the Gram-positive species. The Gram-negative Moraxella catarrhalis and Acinetobacter baumannii were more sensitive than the enterobacteria, but eradication of Pseudomonas aeruginosa in biofilm was insignificant. The influence of the stage of biofilm formation and the initial conditions (bacterial density, photosensitizer concentration and energy fluence of radiation) to the obtained level of inactivation of biofilms was investigated. The PDT with ZnPc photosensitizers show a powerful antimicrobial activity against the most frequent pathogens in endodontic infections and this method for inactivation of pathogens may be used with sucsses for treatment of the bacterial biofilms in the root canals.

  19. PROYECTOS FIN de CARRERA propuestos por el

    E-print Network

    Rey Juan Carlos, Universidad

    -2012 Título: Desarrollo de una plataforma de toma de decisiones online Tutor: César Alfaro (cesar plataforma electrónica de toma de decisiones. Cada unos de estos módulos estarán integrados en una plataforma web para realizar experimentos cognitivos en el ámbito de la toma de decisiones asíncrona por grupos

  20. Antimicrobial activities of cefditoren against respiratory pathogens isolated from children in Japan.

    PubMed

    Seki, H.; Kasahara, Yoshihito; Ohta, Kunio; Ohta, Kazuhide; Saikawa, Yutaka; Sumita, Ryou; Yachie, Akihiro; Fujita Si, Shin-ichi; Koizumi, Shoichi

    1999-03-01

    There is an increasing spread and incidence of penicillin-resistant bacteria that are becoming less susceptible to commonly prescribed oral antimicrobials, including extended-spectrum cephalosporins. Against this background, we undertook this study to determine the prevalence of penicillin resistance in Streptococcus pneumoniae and the in-vitro activity of oral antimitrobials. Between April 1996 and December 1997, in 245 children with respiratory tract infections (bronchitis in 61, pharyngitis in 115, and tonsillitis in 69), 119 strains of Haemophilus influenzae, 89 strains of Streptococcus pyogenes, 61 strains of Streptococcus pneumoniae, 36 strains of Staphylococcus aureus, and 34 strains of Moraxella catarrhalis were isolated from the pharynx. The antimicrobial susceptibility of these isolates was assessed by a broth microdilution method. The isolation incidence of penicillin-intermediately resistant S. pneumoniae (PISP) and penicillin-highly resistant S. pneumoniae (PRSP) was 59.0% and 13.1%, respectively. Most strains of PISP and PRSP were highly resistant to cefaclor, cefpodoxime, cefteram, cefdinir, clarithromycin, ampicillin, and minocycline, but susceptibile to ofloxacin and cefditoren (CDTR). The in-vitro activity of CDTR was superior to that of other cephalosporins, such as cefaclor, cefdinir, and cefpodoxime, when tested against both the beta-lactamase-positive and -negative H. influenzae isolated. CDTR was also active against all the other strains, including methicillin-sensitive S. aureus, S. pyogenes, and M. catarrhalis. This study suggested that CDTR was a useful oral antibiotic for pediatric respiratory tract infections. PMID:11810485

  1. Effects of Vaccination with 10-Valent Pneumococcal Non-Typeable Haemophilus influenza Protein D Conjugate Vaccine (PHiD-CV) on the Nasopharyngeal Microbiome of Kenyan Toddlers

    PubMed Central

    Feazel, Leah M.; Santorico, Stephanie A.; Robertson, Charles E.; Bashraheil, Mahfudh; Scott, J. Anthony G.

    2015-01-01

    Objective Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). Methods Profiles of the nasopharyngeal microbiota of 60 children aged 12-59 months, who had been randomized to receive 2 doses of PHiD-CV (n=30) or Hepatitis A vaccine (n=30) 60 days apart, were constructed by 16S rRNA gene pyrosequencing of swab specimens collected before vaccination and 180 days after dose 1. Results Prior to vaccination, Moraxella catarrhalis (median of 12.3% of sequences/subject), Streptococcus pneumoniae (4.4%) and Corynebacterium spp. (5.6%) were the most abundant nasopharyngeal bacterial species. Vaccination with PHiD-CV did not significantly alter the species composition, abundance, or prevalence of known pathogens. Distinct microbiomes were identified based on the abundances of Streptococcus, Moraxella, and Haemophilus species. These microbiomes shifted in composition over the study period and were independent of age, sex, school attendance, antibiotic exposure, and vaccination. Conclusions Vaccination of children with two doses of PHiD-CV did not significantly alter the nasopharyngeal microbiome. This suggests limited replacement carriage with pathogens other than non-vaccine strains of S. pneumoniae. Trial Registration clinicaltrials.gov NCT01028326 PMID:26083474

  2. Reconstrução tridimensional de arcos magnéticos por tomografia

    NASA Astrophysics Data System (ADS)

    Simões, P. J. A.; Costa, J. E. R.

    2003-08-01

    Uma explosão solar é uma variação súbita do brilho que ocorre nas regiões ativas da atmosfera solar. Estas regiões são constituídas por um plasma magnetizado com intensa indução magnética e em cenários bem complexos como visto recentemente através de experimentos embarcados em satélites operando instrumentos em raios X moles e ultra-violeta distante. A energia magnética, que pode ser armazenada por um período de horas até dias em configurações magnéticas estressadas, é subitamente lançada na atmosfera solar e transferida para partículas como elétrons, prótons e núcleos pesados, que são acelerados e/ou aquecidos, produzindo radiação eletromagnética. A proposta final deste projeto é determinar as características espaciais de alta resolução da emissão e polarização girossincrotrônica de explosões solares em ambientes complexos de campos magnéticos. Os recentes resultados da emissão difusa em EUV apresentado pelos satélites TRACE e SOHO dos arcos magnéticos conectando as diferentes polaridades magnéticas sobre as regiões ativas possibilitam novas abordagens sobre o papel do campo magnético na emissão em rádio. Nesta etapa apresentamos os resultados da reconstrução da geometria tridimensional das linhas de força destes arcos utilizando técnicas tomográficas, a partir de imagens de alta resolução espacial obtidas pelo instrumento EIT (Extreme ultraviolet Imaging Telescope), além da modelagem das induções magnéticas por um campo dipolar e as densidades de partículas aceleradas. Utilizamos para a reconstrução geométrica, imagens tomadas em vários ângulos dos arcos devido à rotacão solar. Com estes resultados, daremos continuidade ao projeto, com os cálculos da transferência radiativa nos modos ordinário e extraordinário de propagação da radiação girossincrotrônica de explosões solares.

  3. Gestión de Riesgo de Desastres por Comunidades y Gobiernos Locales

    Microsoft Academic Search

    Christina Bollin; Camilo Cárdenas; Herwig Hahn; Krishna S. Vatsa

    2003-01-01

    El presente documento forma parte de una serie de estudios comisionados por la Red. El estudio se refiere a la gestión del riesgo de desastres a nivel local. El tema fue seleccionado por los miembros de la Red.

  4. Nutrition, antigenicity and serological characteristics of different strains of Moraxella bovis 

    E-print Network

    Chowdhury, T. I. M. Fazlayrabbi

    1963-01-01

    of M. bovis (Experiment 3). 39 NUTRITION, ANTIGENICITY AND SEROLOGICAL CHARACTERISTICS OF OIFFEREEF SFRAIIFS OF MORAEELIA BASIS INTRODUCTION Infectious keratoconjunctivitis or "pink eye" is a contagious disease which chiefly affects the eyes.... Mittar, S. N. : Contagious Opthalmia Among Cattle. Vet. J. , 71 (1915): 2S. 24. Penberthy, J. : Contagious Opthalmia in Cattle, J. Comp. Path. and Therap. , 10 (1897): 263. 25. Pook, H. L. : Inclusion Cell Conjunctivitis in Calves. Vet. Rec. , 63...

  5. VALIDAO DE METODOLOGIA PARA DETERMINAO DE BTEX EM AMOSTRAS AMBIENTAIS POR GC/MS COM INJEO POR HEADSPACE

    E-print Network

    Ferreira, Márcia M. C.

    VALIDAÇÃO DE METODOLOGIA PARA DETERMINAÇÃO DE BTEX EM AMOSTRAS AMBIENTAIS POR GC/MS COM INJEÇÃO POR São Paulo. Palavras Chaves: BTEX, validação, GC/MS A contaminação de solo e águas subterrâneas por espectrometria de massas (GC/MS), que permite a identificação e quantificação das espécies de interesse com

  6. Detección y estudio mediante Fluorescencia Inducida por Láser de radicales libres formados por Disociación Multifotónica Infrarroja

    NASA Astrophysics Data System (ADS)

    Santos, M.; Díaz, L.; Torresano, J. A.; Rubio, L.; Samoudi, B.

    Una de las principales aplicaciones actuales de los procesos de disociación multifotónica inducidos por radiación láser infrarroja (DMI) es la producción de radiales libres, con el fin de estudiar sus propiedades cinéticas y espectroscópicas. La disociación de moléculas poliatómicas en el IR con láseres de CO2 tiene lugar desde la superficie de energía molecular mas baja y conduce generalmente a la formación de fragmentos en el estado electrónico fundamental, con diversos grados de excitación vibracional. En el Grupo de Procesos Multifotónicos del Instituto de Estructura de la Materia del C.S.I.C. hemos puesto a punto la técnica de Fluorescencia Inducida por Láser (LIF) para la detección y análisis en tiempo real de los fragmentos producidos en la DMI inducida mediante uno o dos campos láseres de diferentes longitudes de onda. Objetivos de nuestro trabajo han sido el estudio de los canales de disociación mayoritarios y de las especies transitoria producidas, así como de la distribución de energía interna con que éstas son generadas. En particular hemos detectado mediante LIF las especies: C2, CF, CH, SiH2, CF2, CH2, SiHCl, y CF3 a partir de la disociación de, entre otras, las siguientes moléculas: C2H3Br, C3F6, C4H8Si, C2H5ClSi y CH5ClSi. En este trabajo presentamos algunos de los resultados obtenidos mediante el estudio por LIF de estos radicales: estudio temporal de la señal LIF obtenida con determinación de tiempos de vida, espectros de excitación y fluorescencia, temperaturas vibracionales de formación, variación de la intensidad LIF con el tiempo de retraso entre los láseres de disociación y prueba, etc.

  7. Especificación de la Demanda por Dinero con Innovación Financiera

    Microsoft Academic Search

    José Eduardo Gómez

    En esta nota se presentan los resultados de la estimación de la demanda por base monetaria-dinero de alto expansivo-en Colombia, introduciendo la relación entre depósitos sujetos a encaje y efectivo como proxy de la variable cambio tecnológico en el sistema financiero. Se muestra que hay tres vectores de cointegración, uno de los cuales puede interpretarse como la demanda por base

  8. Infección por VIH y el riesgo de cáncer

    Cancer.gov

    Hoja informativa que describe el mayor riesgo de ciertos tipos de cánceres que presentan las personas con infección por VIH. Dichos cánceres son el sarcoma de Kaposi, los linfomas de Hodgkin y no Hodgkin, y los cánceres de ano, de cérvix, de hígado y de pulmón. Trata también de lo que las personas con infección por VIH pueden hacer para reducir su riesgo de cáncer o para detectar temprano la enfermedad.

  9. Human Opsonins Induced during Meningococcal Disease Recognize Outer Membrane Proteins PorA and PorB

    Microsoft Academic Search

    A. K. LEHMANN; A. HALSTENSEN; I. S. AABERGE; J. HOLST; T. E. MICHAELSEN; S. SØRNES; L. M. WETZLER; H.-K. GUTTORMSEN

    1999-01-01

    Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) proteins purified from mutants of the same strain (44\\/76; B:15:P1.7.16) were adsorbed to fluorescent beads, opsonized with acute- and convalescent-phase

  10. Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens

    PubMed Central

    Shen, Hongwei; Zhu, Bingqing; Wang, Shulian; Mo, Haolian; Wang, Ji; Li, Jin; Zhang, Chen; Zeng, Huashu; Guan, Li; Shi, Weixian; Zhang, Yong; Ma, Xuejun

    2015-01-01

    A large number of viral and bacterial organisms are responsible for community-acquired pneumonia (CAP) which contributes to substantial burden on health management. A new resequencing microarray (RPM-IVDC1) associated with targeted multiplex PCR was recently developed and validated for multiple respiratory viruses detection and discrimination. In this study, we evaluated the capability of RPM-IVDC1 for simultaneous identification of multiple viral and bacterial organisms. The nasopharyngeal aspirates (NPAs) of 110 consecutive CAP patients, aged from 1 month to 96 years old, were collected from five distinct general hospitals in Beijing during 1-year period. The samples were subjected to the RPM-IVDC1 established protocol as compared to a real-time PCR (qRT-PCR), which was used as standard. The results of virus detection were consistent with those previously described. A total of 37 of Streptococcus pneumoniae, 14 of Haemophilus influenzae, 10 of Mycoplasma pneumoniae, two of Klebsiella pneumoniae and one of Moraxella catarrhalis were detected by RPM-IVDC1. The sensitivities and specificities were compared with those of qRT-PCR for S. pneumoniae (100, 100%, respectively), H. influenzae (92.3, 97.9%, respectively), M. pneumoniae (69.2, 99.0%, respectively), K. pneumoniae (100, 100%, respectively), and M. catarrhalis (100, 100%, respectively). Additional 22 of Streptococcus spp., 24 of Haemophilus spp. and 16 of Neisseria spp. were identified. In addition, methicillin-resistant and carbapenemases allele were also found in nine of Staphylococcus spp. and one of K. pneumoniae, respectively. These results demonstrated the capability of RPM-IVDC1 for simultaneous detection of broad-spectrum respiratory pathogens in complex backgrounds and the advantage of accessing to the actual sequences, showing great potential use of epidemic outbreak investigation. The detection results should be carefully interpreted when introducing this technique in the clinical diagnostics. PMID:26074910

  11. Bacterial and Respiratory Viral Interactions in the Etiology of Acute Otitis Media in HIV-infected and HIV-uninfected South African Children

    PubMed Central

    Govender, Niresha; Dayal, Kishen; Devadiga, Raghavendra; Van Dyke, Melissa K.; van Niekerk, Nadia; Cutland, Clare Louise; Adrian, Peter V.; Nunes, Marta C.

    2015-01-01

    Background: Bacteria and respiratory viruses are implicated in the pathogenesis of acute otitis media (AOM); however, data from low–middle income countries are sparse. We investigated the etiology of AOM in HIV-infected (HIV+), HIV-uninfected (HIV?) and HIV-exposed clinically asymptomatic for HIV-infection (HEU) South African children. Methods: Children ?3 months to <5 years of age with AOM were enrolled between May 2009 and April 2010 (NCT01031082). Middle ear fluid samples were cultured for bacteria; antibacterial susceptibility was done and serotyping undertaken for Streptococcus pneumoniae and Haemophilus influenzae. Nasopharyngeal aspirates were analyzed for respiratory viruses using immunofluorescence assay and polymerase chain reaction. Results: Of 260 AOM episodes (HIV+:15; HIV?:182; HEU:63), bacteria were found in 54.6%, including Haemophilus influenzae (30.8%), 98.8% of which were nontypeable, and Streptococcus pneumoniae (20.4%), Staphylococcus aureus (15.8%), Moraxella catarrhalis (5.0%) and Streptococcus pyogenes (1.5%). Nonsusceptibility of Streptococcus pneumoniae to penicillin was 64.2%. Respiratory viruses were detected in 74.2% of cases. Human rhinovirus was most frequently detected (37.7%), followed by adenovirus (14.2%) and human bocavirus (11.5%) overall and irrespective of HIV status. Respiratory viruses were identified concurrently with S. pneumoniae, H. influenzae, M. catarrhalis (76.9–78.8%) and Staphylococcus aureus (63.4%) cultured from middle ear fluid, as well as in 72.0% of episodes negative for any bacteria. Conclusion: The study suggests that respiratory viruses and pathogenic bacteria play an important role in the development of AOM in children. A similar spectrum of pathogens was observed independently of HIV status. Vaccines targeting both nontypeable Haemophilus influenzae and S. pneumoniae may have a broad impact on AOM in South Africa. PMID:25923426

  12. Bacterial-resistance among outpatients of county hospitals in China: significant geographic distinctions and minor differences between central cities.

    PubMed

    Xiao, Yonghong; Wei, Zeqing; Shen, Ping; Ji, Jinru; Sun, Ziyong; Yu, Hua; Zhang, Tiantuo; Ji, Ping; Ni, Yuxing; Hu, Zhidong; Chu, Yunzhuo; Li, Lanjuan

    2015-06-01

    The purpose of this study was to survey antibacterial resistance in outpatients of Chinese county hospitals. A total of 31 county hospitals were selected and samples continuously collected from August 2010 to August 2011. Drug sensitivity testing was conducted in a central laboratory. A total of 2946 unique isolates were collected, including 634 strains of Escherichia coli, 606 Klebsiella pneumoniae, 476 Staphylococcus aureus, 308 Streptococcus pneumoniae, and 160 Haemophilus influenzae. Extended-spectrum ?-lactamases were detected in E. coli (42.3% strains), K. pneumoniae (31.7%), and Proteus mirabilis (39.0%). Ciprofloxacin-resistance was detected in 51.0% of E. coli strains. Salmonella spp. and Shigella spp. were sensitive to most antibacterial agents. Less than 8.0% of Pseudomonas aeruginosa isolates were resistant to carbapenem. For S. aureus strains, 15.3% were resistant to methicillin, and some strains of S. pneumoniae showed resistance to penicillin (1.6%), ceftriaxone (13.0%), and erythromycin (96.4%). ?-lactamase was produced by 96.5% of Moraxella catarrhalis strains, and 36.2% of H. influenzae isolates were resistant to ampicillin. Azithromycin-resistant H. influenzae, imipenem-resistant but meropenem-sensitive Proteus, and ceftriaxone- and carbapenem non-sensitive M. catarrhalis were recorded. In conclusion, cephalosporin- and quinolone-resistant strains of E. coli and Klebsiella pneumonia and macrolide-resistant Gram-positive cocci were relatively prominent in county hospitals. The antibacterial resistance profiles of isolates from different geographical locations varied significantly, with proportions in county hospitals lower than those in their tertiary counterparts in the central cities, although the difference is diminishing. PMID:25708671

  13. What is behind the ear drum? The microbiology of otitis media and the nasopharyngeal flora in children in the era of pneumococcal vaccination

    PubMed Central

    Mills, Nikki; Best, Emma J; Murdoch, David; Souter, Melanie; Neeff, Michel; Anderson, Trevor; Salkeld, Lesley; Ahmad, Zahoor; Mahadevan, Murali; Barber, Colin; Brown, Colin; Walker, Cameron; Walls, Tony

    2015-01-01

    Aim This study aims to describe the microbiology of middle ear fluid (MEF) in a cohort of children vaccinated with Streptococcus pneumoniae conjugate vaccine (PCV7) having ventilation tube insertion. Nasopharyngeal (NP) carriage of otopathogens in these children is compared with children without history of otitis media. Methods Between May and November 2011, MEF and NP samples from 325 children aged <3 years were collected in three major centres in New Zealand at the time of ventilation tube insertion. An age-matched non-otitis-prone comparison group of 137 children had NP samples taken. A questionnaire was completed by both groups. Results Immunisation coverage with at least one dose of PCV7 was 97%. Haemophilus influenzae was cultured in 19.4% of MEF and was polymerase chain reaction (PCR) positive in 43.4%. S. pneumoniae and Moraxella catarrhalis were cultured in <10% of MEF samples but were PCR positive for 23.1% and 38.7%, respectively. H. influenzae was the most common organism isolated from NP samples (60%) in the grommet group, while M. catarrhalis (56%) was the most common in the non-otitis prone group. S. pneumoniae was more commonly found in the nasopharynx of children with ear disease (41% vs. 29%). 19F was the most prominent S. pneumoniae serotype in NP samples of both groups, but no serotype dominated in MEF. Ninety-five per cent of H. influenzae isolates were confirmed to be non-typeable H. influenzae. Conclusion In this cohort of children with established ear disease requiring surgical intervention, non-typeable H. influenzae is the dominant pathogen in both the nasopharynx and MEF. PMID:25175818

  14. Common Cell Shape Evolution of Two Nasopharyngeal Pathogens.

    PubMed

    Veyrier, Frédéric J; Biais, Nicolas; Morales, Pablo; Belkacem, Nouria; Guilhen, Cyril; Ranjeva, Sylvia; Sismeiro, Odile; Péhau-Arnaudet, Gérard; Rocha, Eduardo P; Werts, Catherine; Taha, Muhamed-Kheir; Boneca, Ivo G

    2015-07-01

    Respiratory infectious diseases are the third cause of worldwide death. The nasopharynx is the portal of entry and the ecological niche of many microorganisms, of which some are pathogenic to humans, such as Neisseria meningitidis and Moraxella catarrhalis. These microbes possess several surface structures that interact with the actors of the innate immune system. In our attempt to understand the past evolution of these bacteria and their adaption to the nasopharynx, we first studied differences in cell wall structure, one of the strongest immune-modulators. We were able to show that a modification of peptidoglycan (PG) composition (increased proportion of pentapeptides) and a cell shape change from rod to cocci had been selected for along the past evolution of N. meningitidis. Using genomic comparison across species, we correlated the emergence of the new cell shape (cocci) with the deletion, from the genome of N. meningitidis ancestor, of only one gene: yacF. Moreover, the reconstruction of this genetic deletion in a bacterium harboring the ancestral version of the locus together with the analysis of the PG structure, suggest that this gene is coordinating the transition from cell elongation to cell division. Accompanying the loss of yacF, the elongation machinery was also lost by several of the descendants leading to the change in the PG structure observed in N. meningitidis. Finally, the same evolution was observed for the ancestor of M. catarrhalis. This suggests a strong selection of these genetic events during the colonization of the nasopharynx. This selection may have been forced by the requirement of evolving permissive interaction with the immune system, the need to reduce the cellular surface exposed to immune attacks without reducing the intracellular storage capacity, or the necessity to better compete for adhesion to target cells. PMID:26162030

  15. Multistep Resistance Development Studies of Ceftaroline in Gram-Positive and -Negative Bacteria?

    PubMed Central

    Clark, Catherine; McGhee, Pamela; Appelbaum, Peter C.; Kosowska-Shick, Klaudia

    2011-01-01

    Ceftaroline, the active component of the prodrug ceftaroline fosamil, is a novel broad-spectrum cephalosporin with bactericidal activity against Gram-positive and -negative isolates. This study evaluated the potential for ceftaroline and comparator antibiotics to select for clones of Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus influenzae, Moraxella catarrhalis, Klebsiella pneumoniae, Staphylococcus aureus, and Enterococcus faecalis with elevated MICs. S. pneumoniae and S. pyogenes isolates in the present study were highly susceptible to ceftaroline (MIC range, 0.004 to 0.25 ?g/ml). No streptococcal strains yielded ceftaroline clones with increased MICs (defined as an increase in MIC of >4-fold) after 50 daily passages. Ceftaroline MICs for H. influenzae and M. catarrhalis were 0.06 to 2 ?g/ml for four strains and 8 ?g/ml for a ?-lactamase-positive, efflux-positive H. influenzae with a mutation in L22. One H. influenzae clone with an increased ceftaroline MIC (quinolone-resistant, ?-lactamase-positive) was recovered after 20 days. The ceftaroline MIC for this isolate increased 16-fold, from 0.06 to 1 ?g/ml. MICs for S. aureus ranged from 0.25 to 1 ?g/ml. No S. aureus isolates tested with ceftaroline had clones with increased MIC (>4-fold) after 50 passages. Two E. faecalis isolates tested had ceftaroline MICs increased from 1 to 8 ?g/ml after 38 days and from 4 to 32 ?g/ml after 41 days, respectively. The parental ceftaroline MIC for the one K. pneumoniae extended-spectrum ?-lactamase-negative isolate tested was 0.5 ?g/ml and did not change after 50 daily passages. PMID:21343467

  16. Common Cell Shape Evolution of Two Nasopharyngeal Pathogens

    PubMed Central

    Veyrier, Frédéric J.; Biais, Nicolas; Morales, Pablo; Belkacem, Nouria; Guilhen, Cyril; Ranjeva, Sylvia; Sismeiro, Odile; Péhau-Arnaudet, Gérard; Rocha, Eduardo P.; Werts, Catherine

    2015-01-01

    Respiratory infectious diseases are the third cause of worldwide death. The nasopharynx is the portal of entry and the ecological niche of many microorganisms, of which some are pathogenic to humans, such as Neisseria meningitidis and Moraxella catarrhalis. These microbes possess several surface structures that interact with the actors of the innate immune system. In our attempt to understand the past evolution of these bacteria and their adaption to the nasopharynx, we first studied differences in cell wall structure, one of the strongest immune-modulators. We were able to show that a modification of peptidoglycan (PG) composition (increased proportion of pentapeptides) and a cell shape change from rod to cocci had been selected for along the past evolution of N. meningitidis. Using genomic comparison across species, we correlated the emergence of the new cell shape (cocci) with the deletion, from the genome of N. meningitidis ancestor, of only one gene: yacF. Moreover, the reconstruction of this genetic deletion in a bacterium harboring the ancestral version of the locus together with the analysis of the PG structure, suggest that this gene is coordinating the transition from cell elongation to cell division. Accompanying the loss of yacF, the elongation machinery was also lost by several of the descendants leading to the change in the PG structure observed in N. meningitidis. Finally, the same evolution was observed for the ancestor of M. catarrhalis. This suggests a strong selection of these genetic events during the colonization of the nasopharynx. This selection may have been forced by the requirement of evolving permissive interaction with the immune system, the need to reduce the cellular surface exposed to immune attacks without reducing the intracellular storage capacity, or the necessity to better compete for adhesion to target cells. PMID:26162030

  17. Nationwide surveillance of bacterial respiratory pathogens conducted by the Surveillance Committee of Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Clinical Microbiology in 2009: general view of the pathogens' antibacterial susceptibility.

    PubMed

    Watanabe, Akira; Yanagihara, Katsunori; Matsumoto, Tetsuya; Kohno, Shigeru; Aoki, Nobuki; Oguri, Toyoko; Sato, Junko; Muratani, Tetsuro; Yagisawa, Morimasa; Ogasawara, Kazuhiko; Koashi, Naoto; Kozuki, Tsuneo; Komoto, Akira; Takahashi, Yoshisaburo; Tsuji, Toshikatsu; Terada, Michinori; Nakanishi, Kunio; Hattori, Rikizo; Hirako, Yukio; Maruo, Akinori; Minamitani, Shinichi; Morita, Kohei; Wakamura, Tomotaro; Sunakawa, Keisuke; Hanaki, Hideaki; Ohsaki, Yoshinobu; Honda, Yasuhito; Sasaoka, Shoichi; Takeda, Hiroaki; Ikeda, Hideki; Sugai, Atsuko; Miki, Makoto; Nakanowatari, Susumu; Takahashi, Hiroshi; Utagawa, Mutsuko; Kobayashi, Nobuyuki; Takasaki, Jin; Konosaki, Hisami; Aoki, Yasuko; Shoji, Michi; Goto, Hajime; Saraya, Takeshi; Kurai, Daisuke; Okazaki, Mitsuhiro; Kobayashi, Yoshio; Katono, Yasuhiro; Kawana, Akihiko; Saionji, Katsu; Miyazawa, Naoki; Sato, Yoshimi; Watanuki, Yuji; Kudo, Makoto; Ehara, Shigeru; Tsukada, Hiroki; Imai, Yumiko; Watabe, Nobuei; Aso, Sakura; Honma, Yasuo; Mikamo, Hiroshige; Yamagishi, Yuka; Takesue, Yoshio; Wada, Yasunao; Nakamura, Tadahiro; Mitsuno, Noriko; Mikasa, Keiichi; Kasahara, Kei; Uno, Kenji; Sano, Reiko; Miyashita, Naoyuki; Kurokawa, Yukinori; Takaya, Mariko; Kuwabara, Masao; Watanabe, Yaeko; Doi, Masao; Shimizu, Satomi; Negayama, Kiyoshi; Kadota, Junichi; Hiramatsu, Kazufumi; Morinaga, Yoshitomo; Honda, Junichi; Fujita, Masaki; Iwata, Satoshi; Iwamoto, Aikichi; Ezaki, Takayuki; Onodera, Shoichi; Kusachi, Shinya; Tateda, Kazuhiro; Tanaka, Michio; Totsuka, Kyoichi; Niki, Yoshihito; Matsumoto, Tetsuro

    2012-10-01

    For the purpose of nationwide surveillance of antimicrobial susceptibility of bacterial respiratory pathogens from patients in Japan, the Japanese Society of Chemotherapy (JSC) started a survey in 2006. From 2009, JSC continued the survey in collaboration with the Japanese Association for Infectious Diseases and the Japanese Society for Clinical Microbiology. The fourth-year survey was conducted during the period from January and April 2009 by the three societies. A total of 684 strains were collected from clinical specimens obtained from well-diagnosed adult patients with respiratory tract infections. Susceptibility testing was evaluable with 635 strains (130 Staphylococcus aureus, 127 Streptococcus pneumoniae, 4 Streptococcus pyogenes, 123 Haemophilus influenzae, 70 Moraxella catarrhalis, 78 Klebsiella pneumoniae, and 103 Pseudomonas aeruginosa). A maximum of 45 antibacterial agents including 26 ?-lactams (four penicillins, three penicillins in combination with ?-lactamase inhibitors, four oral cephems, eight parenteral cephems, one monobactam, five carbapenems, and one penem), four aminoglycosides, four macrolides (including ketolide), one lincosamide, one tetracycline, two glycopeptides, six fluoroquinolones, and one oxazolidinone were used for the study. Analysis was conducted at the central reference laboratory according to the method recommended by the Clinical and Laboratory Standard Institute (CLSI). Incidence of methicillin-resistant S. aureus (MRSA) was as high as 58.5 %, and that of penicillin-intermediate and penicillin-resistant S. pneumoniae (PISP and PRSP) was 6.3 % and 0.0 %, respectively. Among H. influenzae, 21.1 % of them were found to be ?-lactamase-non-producing ampicillin (ABPC)-intermediately resistant (BLNAI), 18.7 % to be ?-lactamase-non-producing ABPC-resistant (BLNAR), and 5.7 % to be ?-lactamase-producing ABPC-resistant (BLPAR) strains. A high frequency (76.5 %) of ?-lactamase-producing strains has been suspected in Moraxella catarrhalis isolates. Four (3.2 %) extended-spectrum ?-lactamase-producing K. pneumoniae were found among 126 strains. Four isolates (2.5 %) of P. aeruginosa were found to be metallo-?-lactamase-producing strains, including three (1.9 %) suspected multi-drug resistant strains showing resistance against imipenem, amikacin, and ciprofloxacin. Continuous national surveillance of the antimicrobial susceptibility of respiratory pathogens is crucial to monitor changing patterns of susceptibility and to be able to update treatment recommendations on a regular basis. PMID:22766652

  18. System design description for portable 1,000 CFM exhauster Skids POR-007/Skid E and POR-008/Skid F

    SciTech Connect

    Nelson, O.D.

    1998-07-25

    The primary purpose of the two 1,000 CFM Exhauster Skids, POR-007-SKID E and POR-008-SKID F, is to provide backup to the waste tank primary ventilation systems for tanks 241-C-106 and 241-AY-102, and the AY-102 annulus in the event of a failure during the sluicing of tank 241-C-106 and subsequent transfer of sluiced waste to 241-AY-102. This redundancy is required since both of the tank ventilation systems have been declared as Safety Class systems.

  19. PRIMAS POR PLAZO EN EL MERCADO ESPAÑOL DE DEUDA PÚBLICA

    Microsoft Academic Search

    PAZ RICO

    1999-01-01

    En este trabajo se realiza un análisis de las primas por plazo en el merca- do español de deuda pública, durante el período que abarca desde enero de 1991 a diciembre de 1995. El objetivo es determinar la hipótesis que explica la relación entre los tipos de interés a corto y largo plazo en el mercado de deuda pública. En

  20. Por favor reporte su encuentro con el Pez-Sierra!

    E-print Network

    Watson, Craig A.

    Especies amenazadas (Endangered Species Act). Sus poblaciones han sufrido una fuerte reducción, por lo que-8230 International Sawfish Encounter Database Florida Program for Shark Research Florida Museum of Natural History Dickinson Hall Museum Road PO Box 117800 Gainesville FL 3261-17800 sawfish@flmnh.ufl.edu http://www.flmnh.ufl.edu/fish/sharks

  1. DEPARTAMENTO DE DIREITO EDITAL PARA CONCURSO DE ADMISSO POR TRANSFERNCIA

    E-print Network

    DEPARTAMENTO DE DIREITO EDITAL PARA CONCURSO DE ADMISSÃO POR TRANSFERÊNCIA EXTERNA E PORTADORES DE: - estar cursando até o 5º (quinto) período de Direito ou equivalente, nos casos de cursos seriados; - uma curso de Direito da PUC-Rio. (b) de IES estrangeira: - uma declaração pessoal, descrevendo os motivos de

  2. DEPARTAMENTO DE DIREITO EDITAL PARA CONCURSO DE ADMISSO POR TRANSFERNCIA

    E-print Network

    DEPARTAMENTO DE DIREITO EDITAL PARA CONCURSO DE ADMISSÃO POR TRANSFERÊNCIA INTERNA, TRANSFERÊNCIA PUC-Rio a disciplina Introdução ao Direito I (JUR 1031), logrando-se aprovação na mesma. 1. Da prova objetivas envolvendo conteúdo da disciplina Introdução à Ciência do Direito I; (b) dissertação a respeito do

  3. DEPARTAMENTO DE DIREITO EDITAL PARA CONCURSO DE ADMISSO POR TRANSFERNCIA

    E-print Network

    DEPARTAMENTO DE DIREITO EDITAL PARA CONCURSO DE ADMISSÃO POR TRANSFERÊNCIA INTERNA E TRANSFERÊNCIA Introdução ao Direito I (JUR 1031), logrando-se aprovação na mesma. 1. Da prova de seleção. A prova de disciplina Introdução à Ciência do Direito I; (b) dissertação a respeito do seguinte texto1 : BOBBIO

  4. Identification of Porphyromonas gingivalis proteins secreted by the Por secretion system.

    PubMed

    Sato, Keiko; Yukitake, Hideharu; Narita, Yuka; Shoji, Mikio; Naito, Mariko; Nakayama, Koji

    2013-01-01

    The Gram-negative bacterium Porphyromonas gingivalis possesses a number of potential virulence factors for periodontopathogenicity. In particular, cysteine proteinases named gingipains are of interest given their abilities to degrade host proteins and process other virulence factors such as fimbriae. Gingipains are translocated on the cell surface or into the extracellular milieu by the Por secretion system (PorSS), which consists of a number of membrane or periplasmic proteins including PorK, PorL, PorM, PorN, PorO, PorP, PorQ, PorT, PorU, PorV (PG27, LptO), PorW and Sov. To identify proteins other than gingipains secreted by the PorSS, we compared the proteomes of P. gingivalis strains kgp rgpA rgpB (PorSS-proficient strain) and kgp rgpA rgpB porK (PorSS-deficient strain) using two-dimensional gel electrophoresis and peptide-mass fingerprinting. Sixteen spots representing 10 different proteins were present in the particle-free culture supernatant of the PorSS-proficient strain but were absent or faint in that of the PorSS-deficient strain. These identified proteins possessed the C-terminal domains (CTDs), which had been suggested to form the CTD protein family. These results indicate that the PorSS is used for secretion of a number of proteins other than gingipains and that the CTDs of the proteins are associated with the PorSS-dependent secretion. PMID:23075153

  5. Son decenas y estn por toda Amrica Latina. Tienen en comn su amor por la ciencia y una curiosidad del tamao

    E-print Network

    Barbosa, Marcia C. B.

    Son decenas y están por toda América Latina. Tienen en común su amor por la ciencia y una la Red Inter-Americana de Academias de Ciencias (IANAS, por sus siglas en inglés), la Unesco, la ONU mujeres que lideran la ciencia en América Latina Margarita Rodríguez BBC Mundo Miércoles, 16 de octubre de

  6. PERMISO CUIDADO DE HIJO MENOR AFECTADO POR CNCER U OTRA ENFERMEDAD GRAVE.

    E-print Network

    Bernabé García, Gregorio

    , del hijo menor de edad, por naturaleza o adopción, o en los supuestos de acogimiento preadoptivo oPERMISO CUIDADO DE HIJO MENOR AFECTADO POR CÁNCER U OTRA ENFERMEDAD GRAVE. La ley 39/2010, de 22 de redacción: e) Permiso por cuidado de hijo menor afectado por cáncer u otra enfermedad grave: el funcionario

  7. Antibacterial activity of RU 64004 (HMR 3004), a novel ketolide derivative active against respiratory pathogens.

    PubMed Central

    Agouridas, C; Bonnefoy, A; Chantot, J F

    1997-01-01

    The antibacterial activity of RU 64004, a new ketolide, was evaluated against more than 600 bacterial strains and was compared with those of various macrolides and pristinamycin. RU 64004 had good activity against multiresistant pneumococci, whether they were erythromycin A resistant or not, including penicillin-resistant strains. RU 64004 inhibited 90% of pneumococci resistant to erythromycin A and penicillin G at 0.6 and 0.15 microg/ml, respectively. Unlike macrolides, RU 64004 did not induce the phenotype of resistance to macrolides-lincosamides-streptogramin B. Its good antibacterial activity against multiresistant pneumococci ran in parallel with its well-balanced activity against all bacteria involved in respiratory infections (e.g., Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pyogenes). In contrast to all comparators (14- and 16-membered-ring macrolides and pristinamycin), RU 64004 displayed high therapeutic activity in animals infected with all major strains, irrespective of the phenotypes of the strains. The results suggest that RU 64004 has potential for use in the treatment of infections caused by respiratory pathogens including multiresistant pneumococci. PMID:9333040

  8. The tetrasaccharide L-alpha-D-heptose1-->2-L-alpha-D-heptose1--> 3-L-alpha-D-heptose1-->(3-deoxy-D-manno-octulosonic acid) and phosphate in lipid A define the conserved epitope in Haemophilus lipopolysaccharides recognized by a monoclonal antibody.

    PubMed Central

    Borrelli, S; Hegedus, O; Shaw, D H; Jansson, P E; Lindberg, A A

    1995-01-01

    A murine monoclonal antibody, MAHI 3 (immunoglobulin G2b), that is broadly reactive with Haemophilus influenzae lipopolysaccharides (LPSs) but nonreactive with all enterobacterial LPSs tested was generated by fusing mouse myeloma cells with spleen cells of BALB/c mice immunized with azide-killed H. influenzae RM.7004. MAHI 3 bound to all H. influenzae, all other human Haemophilus spp., all Bordetella pertussis and Bordetella parapertussis, and all Aeromonas spp. tested but not to any Neisseria or Moraxella catarrhalis strains, as determined by enzyme immunoassay, colony dot immunoblotting, and immunoblotting. In an inhibition enzyme immunoassay, MAHI 3 reacted with all 45 H. influenzae LPSs tested but not with the LPS from the rough mutant I69 Rd-/b+, which has only 3-deoxy-D-manno-octulosonic acid (P) [Kdo(P)] and lipid A. The antibody was not inhibited by H. influenzae lipid A or lipid-free polysaccharide isolated after mild acid hydrolysis. Only native LPSs show positive inhibitory activity, indicating that part of lipid A is involved in the binding of MAHI 3. From the results, it is indicated that the structural element recognized by MAHI 3 is Hep alpha 1-->2Hep alpha 1-->3Hep alpha 1-->Kdo together with part of lipid A, including the phosphate. PMID:7543887

  9. Comparative evaluation of loracarbef and amoxicillin-clavulanate for acute otitis media.

    PubMed Central

    Gan, V N; Kusmiesz, H; Shelton, S; Nelson, J D

    1991-01-01

    One hundred five infants and children with acute otitis media were randomized to therapy with loracarbef, an experimental carbacephem antibiotic, or amoxicillin-clavulanate (Augmentin), an approved drug for this disease. Ninety-two were evaluable (46 in each group). Middle ear fluid samples obtained for culture before therapy grew Haemophilus spp. in 30% of cases, pneumococci in 29% of cases, and Moraxella catarrhalis in 15% of cases. beta-Lactamase-producing bacteria were found in 37% of patients. Clinical failure occurred in four loracarbef-treated patients and one amoxicillin-clavulanate-treated patient (P = 0.361). Recurrence of acute otitis media was more common in the 2 to 3 weeks after loracarbef treatment (eight patients) than it was after amoxicillin-clavulanate therapy (three patients), but not significantly so (P = 0.197). Thus, combined failure and recurrence occurred in 12 loracarbef-treated patients and four amoxicillin-clavulanate-treated patients (P = 0.052). Gastrointestinal side effects occurred in 13 loracarbef-treated and 21 amoxicillin-clavulanate-treated patients (P = 0.13). Diaper rash was more common with amoxicillin-clavulanate (22 patients) than with loracarbef (10 patients; P = 0.016). Satisfactory results were achieved with both antibiotics, and adverse effects, although common, were minor. PMID:1854178

  10. Pathogens in the middle ear effusion of children with persistent otitis media: implications of drug resistance and complications.

    PubMed

    Li, W C; Chiu, N C; Hsu, C H; Lee, K S; Hwang, H K; Huang, F Y

    2001-09-01

    Acute otitis media (AOM) is the most common disease for which pediatricians prescribe antimicrobial agents. Middle ear fluid were collected from 243 children with AOM that failed to respond to a previous course of antimicrobial therapy and who had then received myringotomy from September 1997 through August 1999. Bacterial cultures were done and antimicrobial susceptibilities were analyzed. Streptococcus pneumoniae (21.8%) was the most common causative organism, followed by Haemophilus influenzae (10.2%), Staphylococcus aureus (7%), and Pseudomonas aeruginosa (1.8%), while Moraxella catarrhalis (0.7%) and group A beta-hemolytic streptococcus (0.2%) were rarely isolated. In patients whose condition failed to improve after a course of antibiotic treatment, drug resistance became a serious problem. Fourteen percent of the patients in this series had complications, which included recurrent AOM, persistent middle ear effusion necessitating ventilation tube insertion, hearing impairment, mastoiditis, meningitis, chronic otitis media, brain abscess, and sepsis. Possible risk factors such as young age, male sex, underlying diseases, and a culture of S. pneumoniae or H. influenzae were not significantly associated with an increased incidence of complications. More stringent diagnosis and the correct choice of antibiotic treatment combined with the introduction of potential virus and bacterial vaccines are promising ways to reduce the morbidity of AOM in children. PMID:11605810

  11. Diagnosis and treatment of otitis media.

    PubMed

    Ramakrishnan, Kalyanakrishnan; Sparks, Rhonda A; Berryhill, Wayne E

    2007-12-01

    Diagnostic criteria for acute otitis media include rapid onset of symptoms, middle ear effusion, and signs and symptoms of middle ear inflammation. Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are the most common bacterial isolates from the middle ear fluid of children with acute otitis media. Fever, otalgia, headache, irritability, cough, rhinitis, listlessness, anorexia, vomiting, diarrhea, and pulling at the ears are common, but nonspecific symptoms. Detection of middle ear effusion by pneumatic otoscopy is key in establishing the diagnosis. Observation is an acceptable option in healthy children with mild symptoms. Antibiotics are recommended in all children younger than six months, in those between six months and two years if the diagnosis is certain, and in children with severe infection. High-dosage amoxicillin (80 to 90 mg per kg per day) is recommended as first-line therapy. Macrolide antibiotics, clindamycin, and cephalosporins are alternatives in penicillin-sensitive children and in those with resistant infections. Patients who do not respond to treatment should be reassessed. Hearing and language testing is recommended in children with suspected hearing loss or persistent effusion for at least three months, and in those with developmental problems. PMID:18092706

  12. Otopathogens Detected in Middle Ear Fluid Obtained during Tympanostomy Tube Insertion: Contrasting Purulent and Non-Purulent Effusions

    PubMed Central

    Holder, Robert C.; Kirse, Daniel J.; Evans, Adele K.; Whigham, Amy S.; Peters, Timothy R.; Poehling, Katherine A.; Swords, William E.; Reid, Sean D.

    2015-01-01

    Otitis media is a prominent disease among children. Previous literature indicates that otitis media is a polymicrobial disease, with Haemophilus influenzae, Streptococcus pneumoniae, Alloiococcus otitidis and Moraxella catarrhalis being the most commonly associated bacterial pathogens. Recent literature suggests that introduction of pneumococcal conjugate vaccines has had an effect on the etiology of otitis media. Using a multiplex PCR procedure, we sought to investigate the presence of the aforementioned bacterial pathogens in middle ear fluid collected from children undergoing routine tympanostomy tube placement at Wake Forest Baptist Medical Center during the period between January 2011 and March 2014. In purulent effusions, one or more bacterial organisms were detected in ~90% of samples. Most often the presence of H. influenzae alone was detected in purulent effusions (32%; 10 of 31). In non-purulent effusions, the most prevalent organism detected was A. otitidis (26%; 63 of 245). Half of the non-purulent effusions had none of these otopathogens detected. In purulent and non-purulent effusions, the overall presence of S. pneumoniae was lower (19%; 6 of 31, and 4%; 9 of 245, respectively) than that of the other pathogens being identified. The ratio of the percentage of each otopathogen identified in purulent vs. non-purulent effusions was >1 for the classic otopathogens but not for A. otitidis. PMID:26039250

  13. In vitro antibacterial activities of PD 131628, a new 1,8-naphthyridine anti-infective agent.

    PubMed Central

    Cohen, M A; Huband, M D; Mailloux, G B; Yoder, S L; Roland, G E; Domagala, J M; Heifetz, C L

    1991-01-01

    PD 131628 is a new aminopyrrolidine-substituted fluorocyclopropyl naphthyridine quinolone which possesses high in vitro activity against a wide spectrum of bacterial species. The MICs for greater than or equal to 90% of strains were 0.125 to 0.25 microgram/ml for staphylococci, Streptococcus pyogenes, and S. pneumoniae; 0.5 micrograms/ml for S. agalactiae and Enterococcus faecalis; 0.125 micrograms/ml for members of the family Enterobacteriaceae and Acinetobacter spp.; 0.5 micrograms/ml for Pseudomonas aeruginosa; and less than or equal to 0.03 micrograms/ml for Haemophilus influenzae, Moraxella (Branhamella) catarrhalis, and Neisseria gonorrhoeae. In these in vitro comparisons with ciprofloxacin, PD 131628 is more active against gram-positive organisms, approximately equivalent against gram-negative organisms, and, like most other quinolones, relatively inactive against gram-negative anaerobes. In most instances, the in vitro potency of PD 131628 exceeded those of widely used compounds: ciprofloxacin, imipenem, ampicillin, penicillin G, oxacillin, cefazolin, ceftazidime, cefoxitin, cefsulodin, aztreonam, piperacillin, amikacin, spectinomycin, doxycycline, erythromycin, metronidazole, and vancomycin. PMID:2014970

  14. Changing epidemiology of infections in patients with neutropenia and cancer: emphasis on gram-positive and resistant bacteria.

    PubMed

    Zinner, S H

    1999-09-01

    Over the past 3 decades, considerable changes have occurred in the types of bacteria causing infection in febrile patients with neutropenia and cancer. Twenty years ago, gram-negative bacteria caused approximately 70% of bloodstream infections. As a probable consequence of long-dwelling intravascular devices, fluoroquinolone prophylaxis, and high-dose chemotherapy-induced mucositis, there has been a shift toward gram-positive coccal bacteremia. In most centers today, approximately 70% of bacteremic isolates are gram-positive cocci. Of potential concern is that antimicrobial-resistant gram-positive organisms are becoming increasingly frequent in patients with neutropenia. Fluoroquinolone-resistant Escherichia coli are being isolated from several cancer centers. Several "new" organisms, such as Stomatococcus mucilaginosus, Bacillus cereus, Leuconostoc species, Corynebacterium jeikeium, Rhodococcus species, Stenotrophomonas maltophilia, Moraxella catarrhalis, Burkholderia cepacia, and Bartonella species, now cause infections in these patients. Careful application of infection-control principles, judicious prophylaxis, appropriate evaluation of new antibiotics, and prompt effective therapy will maximize benefits for these patients. PMID:10530434

  15. Otopathogens Detected in Middle Ear Fluid Obtained during Tympanostomy Tube Insertion: Contrasting Purulent and Non-Purulent Effusions.

    PubMed

    Holder, Robert C; Kirse, Daniel J; Evans, Adele K; Whigham, Amy S; Peters, Timothy R; Poehling, Katherine A; Swords, William E; Reid, Sean D

    2015-01-01

    Otitis media is a prominent disease among children. Previous literature indicates that otitis media is a polymicrobial disease, with Haemophilus influenzae, Streptococcus pneumoniae, Alloiococcus otitidis and Moraxella catarrhalis being the most commonly associated bacterial pathogens. Recent literature suggests that introduction of pneumococcal conjugate vaccines has had an effect on the etiology of otitis media. Using a multiplex PCR procedure, we sought to investigate the presence of the aforementioned bacterial pathogens in middle ear fluid collected from children undergoing routine tympanostomy tube placement at Wake Forest Baptist Medical Center during the period between January 2011 and March 2014. In purulent effusions, one or more bacterial organisms were detected in ~90% of samples. Most often the presence of H. influenzae alone was detected in purulent effusions (32%; 10 of 31). In non-purulent effusions, the most prevalent organism detected was A. otitidis (26%; 63 of 245). Half of the non-purulent effusions had none of these otopathogens detected. In purulent and non-purulent effusions, the overall presence of S. pneumoniae was lower (19%; 6 of 31, and 4%; 9 of 245, respectively) than that of the other pathogens being identified. The ratio of the percentage of each otopathogen identified in purulent vs. non-purulent effusions was >1 for the classic otopathogens but not for A. otitidis. PMID:26039250

  16. The British Society for Antimicrobial Chemotherapy Resistance Surveillance Project: a successful collaborative model.

    PubMed

    White, Anthony R

    2008-11-01

    The British Society for Antimicrobial Chemotherapy (BSAC) Resistance Surveillance Project was initiated in light of the need for UK-wide surveillance of antibacterial resistance in key clinical pathogens. The Project comprises two defined-protocol programmes that cover a range of important pathogens and antibacterials related to community-acquired respiratory tract infection and bloodstream infection, respectively. The Respiratory Programme has reported quantitative susceptibility data for Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis collected from across the UK and Ireland since 1999. The Bacteraemia Programme has reported the susceptibility of a wide range of Gram-positive and -negative organisms since 2001. The sustainability of the Programmes relies on a unique collaborative funding model: sponsorship is provided by a number of pharmaceutical companies in return for the inclusion of their investigational or marketed agents in the study alongside a core panel of established antibacterials. The sponsors have changed over time according to their interest in participating. Results for marketed agents are communicated in a timely manner through the BSAC web site and by presentation and publication, and for investigational agents with the agreement of their sponsors. The Project satisfies the requirement for sustainable defined-protocol high-quality resistance surveillance across the UK and Ireland. PMID:18819978

  17. Deletion of porA by recombination between clusters of repetitive extragenic palindromic sequences in neisseria meningitidis

    Microsoft Academic Search

    A. VAN DER ENDE; C. T. P. Hopman; J. Dankert

    1999-01-01

    PorA is an important component in a vaccine against infection with Neisseria meningitidis. However, porA- negative meningococci were isolated from patients, thereby potentially limiting the role of PorA-mediated immunity. To analyze the mechanism by which the porA deletion occurred, the regions upstream and down- stream of porA from three meningococcal strains (H44\\/76, H355, and 860183) were sequenced. The porA up-

  18. Erros comuns de ingls em artigos cientficos escritos por pessoas de lngua portuguesa

    E-print Network

    Song, Siang Wun

    Erros comuns de inglês em artigos científicos escritos por pessoas de língua portuguesa Siang Wun comuns de inglês em artigos científicos escritos por pessoa #12;Introdução Baseado em experiência acumulada ao longo dos anos, ao revisar artigos escritos em inglês principalmente por pessoas de língua

  19. Facultade de Ciencias da Educacin Mster Universitario en Didcticas Especficas por la Universidad de A Corua

    E-print Network

    Fraguela, Basilio B.

    Facultade de Ciencias da Educación Máster Universitario en Didácticas Específicas por la A CORUÑA Memoria de Verificación Facultad de Ciencias de la Educación Universidad de A Coruña Año 2013 #12;Facultade de Ciencias da Educación Máster Universitario en Didácticas Específicas por la Universidad de

  20. Facultade de Ciencias da Educacin Mster Universitario en Psicopedagoga por la Universidad de A Corua

    E-print Network

    Fraguela, Basilio B.

    Facultade de Ciencias da Educación Máster Universitario en Psicopedagogía por la Universidad de Verificación Facultad de Ciencias de la Educación Universidad de A Coruña Año 2013 #12;Facultade de Ciencias da Ciencias da Educación Máster Universitario en Psicopedagogía por la Universidad de A Coruña Página 3 de 134

  1. SIMILITUDES ENTRE LAS EMPRESAS QUE IMPLEMENTAN LA CONTABILIDAD POR ÁREAS DE RESPONSABILIDAD

    Microsoft Academic Search

    Martha Ríos Manríquez; Jacobo Oñate Martínez; Enrique Ramos Juárez

    2008-01-01

    Este estudio forma parte de un proyecto que analiza los sistemas de contabilidad utilizadas por las empresas del municipio de Celaya, Guanajuato, México. En este reporte se presenta un análisis comparativo a cuatro empresas que tienen implementada la contabilidad por áreas de responsabilidad, evaluando la utilización de herramientas informáticas, sistemas de información, su relación con el sistema y los objetivos

  2. Se busca reducir de forma drástica la exposición a la radiación en los exámenes por imágenes

    Cancer.gov

    La exposición a la radiación proveniente de exámenes médicos por imaginología en los Estados Unidos ha aumentado en forma significativa. El NCI y diversas organizaciones patrocinaron una reunión en 2011 para promover esfuerzos para reducir la exposición a la radiación por imaginología.

  3. CINÉTICA DE ABSORÇÃO DE NUTRIENTES POR ALFACE EM HIDROPONIA - NFT - COM DUAS CONCENTRAÇÕES DA SOLUÇÃO NUTRITIVA

    Microsoft Academic Search

    G. C. S. Matias; N. N. Cometti; M. S. Fernandes

    A absorção de nutrientes pelas plantas pode ser descrita por parâmetros cinéticos: Vmax (máxima velocidade de absorção do nutriente por unidade de raiz), Km (concentração externa na qual a velocidade de absorção é a metade da máxima) e Cmin (concentração externa na qual o fluxo líquido de íons é zero). O uso de parâmetros cinéticos pode auxiliar tanto na seleção

  4. La Universidad de Alicante expone en Madrid maquetas de arquitecturas singulares hechas por sus alumnos

    E-print Network

    Escolano, Francisco

    La Universidad de Alicante expone en Madrid maquetas de arquitecturas singulares hechas por sus alumnos Alicante, 11 de marzo de 2013 Un total de 72 piezas componen la exposición montada en Madrid por porticadas. La Fundación Cultural COAM de Madrid tiene como fin principal la promoción, difusión y

  5. Règimen legal, tributario, contable y social de las sociedades por acciones simplificadas SAS

    Microsoft Academic Search

    CONSTANZA LORETH FAJARDO CALDERÒN; MYRIAM ROMERO RESTREPO; CARLOS ANDRES VÈLEZ ROMERO

    2010-01-01

    Resumen La SAS, “Sociedad por Acciones Simplificada”, regulada por la Ley 1258 de diciembre 5 de 2008, no es una reforma al Estatuto Mercantil sino un complemento y modernización del mismo. Surge como una nueva realidad societaria que permite el desarrollo de la inversión extranjera,y a la que podrá recurrir el empresario en la estructuración de nuevos negocios en Colombia.

  6. La Neurociencia Computacional hoy: I. Qu es y por qu es difcil su estudio Jess Corts

    E-print Network

    Cortes, Jesus

    La Neurociencia Computacional hoy: I. Qué es y por qué es difícil su estudio Jesús Cortés Institute: Neurociencia, Inteligencia Artificial. Etiquetas: Neurociencia Computacional, simulación por ordenador, procesos neurales, cerebro. La Neurociencia Computacional es una disciplina consolidada, con más de 20 años

  7. Avaliação tardia por tomografia computadorizada espiral de enxertos de pericárdio bovino liso como substitutos aórticos

    Microsoft Academic Search

    Renato A. K. Kalil; Carlos Jader Feldman; Fernando W. B. Ludwig; Alessandro D'Avila da Silva; Paulo Roberto Prates; João R. M. Sant'Anna; Ivo A. Nesralla

    1997-01-01

    Objetivo - Avaliar, morfologicamente, por tomo- grafia computadorizada espiral, enxertos de pericárdio bovino liso empregados como substitutos aórticos. Métodos - Dez pacientes foram submetidos a exame por tomografia computadorizada espiral para reconsti- tuição da imagem dos enxertos. Os critérios de seleção fo- ram tempo de seguimento superior a 2 anos, enxertos de pericárdio liso não revestido, implantados na aorta ascen-

  8. O perfil do médico em formação em radiologia e diagnóstico por imagem

    Microsoft Academic Search

    Glauce Cerqueira Corrêa da Silva; Hilton Augusto Koch; Evandro Guimarães de Sousa

    2007-01-01

    OBJETIVO: Identificar diferenças no perfil psicossocial do especializando e médico residente em radiologia e diagnóstico por imagem, avaliar a aspiração profissional e as motivações internas que os influenciaram a escolher a especialidade radiologia e diagnóstico por imagem e se o nível de satisfação profissional melhora a relação médico-paciente. MATERIAIS E MÉTODOS: Foi aplicado um questionário com 26 perguntas que constava

  9. ESTUDO DAS INCLUSÕES NÃO-METÁLICAS FORMADAS NO METAL DE SOLDA DEPOSITADO POR ARCO SUBMERSO

    Microsoft Academic Search

    M. R. V. de Araújo; W. J. Yamakami; R. C. Tokimatsu; V. A. Ventrella; J. Gallego

    RESUMO Durante a soldagem por arco submerso o metal de solda é isolado por uma camada de fluxo granulado, responsável pela proteção química contra a oxidação e controle de composição química e da microestrutura do metal de solda. No presente trabalho foi realizado um estudo sobre a influência das inclusões formadas em cordões de solda depositados em chapas de aço

  10. Astronomy in the Classroom: Why? (Spanish Title: Astronomía en la Clase: ¿Por Qué?) Astronomia na Sala de Aula: Por Quê?

    NASA Astrophysics Data System (ADS)

    Daros Gama, Leandro; Bagdonas Henrique, Alexandre

    2010-07-01

    There are many discussions about the relevance of the topics covered in classes. One subject in particular is the focus of this essay: astronomy. In what sense and to what extent it would be worth to teach it in science or other kind of classes? In this paper we discuss some aspects of the advantages of dealing with this area of knowledge in schools, taking into account the epistemological and axiological dimensions of astronomy, in light of the vision of science as an intelligent dialogue with the world (Bachelard), in addition to the "problematization" knowledge of Paulo Freire. We propose that in fact the Astronomy does not need to be seen as just a new set of contents to be taught, but appears as a set of motivational contents for historical-philosophical discussions, and permit the discussion of concepts of other disciplines. Numerosas discusiones se están llevando a cabo acerca de la pertinencia de los temas tradicionalmente tratados en las clases. Uno de los temas, en particular, es el foco de este ensayo: la astronomía. ¿En qué sentido y en qué medida sería conveniente tratarla en clase, ya sea en clases de ciencias naturales, específicamente en las de astronomía o asignaturas afines? Elaboramos en este artículo algunos aspectos de las ventajas de tratar esta área del conocimiento en las escuelas, teniendo en cuenta las dimensiones epistemológica y axiológica de la astronomía, a la luz de la visión de la ciencia como un diálogo inteligente con el mundo (Bachelard), además de la propuesta del conocimiento "problematizador" de Paulo Freire. Proponemos que en realidad la astronomía no tiene por qué ser vista sólo como un nuevo conjunto de contenidos que se enseñan, sino que aparece como un conjunto de temas de motivación para el debate histórico-filosófico y para permitir la discusión de los conceptos típicos de otras disciplinas. Muitas discussões vêm acontecendo sobre a relevância dos temas abordados em sala de aula. Um tema, em particular, é foco deste ensaio: a astronomia. Em que sentido e em que medida valeria a pena abordá-la em aulas, seja de ciências, seja de astronomia especificamente, ou em disciplinas afins? Discutimos neste texto alguns aspectos das vantagens de se tratar essa área do conhecimento nas escolas, levando em conta as dimensões epistemológica e axiológica da astronomia, à luz da visão da ciência como um diálogo inteligente com o mundo, de Bachelard, além da proposta de problematização do conhecimento, de Paulo Freire. Proporemos que de fato a astronomia não precisa ser vista como apenas um novo conjunto de conteúdos a serem ensinados, mas figura como conjunto de temas motivadores para discussões histórico-filosóficas, além de permitir a abordagem de conceitos típicos de outras disciplinas.

  11. PUENTES ENTRE LA LITERATURA Y LAS ARTES PLSTICAS. CURSO DE EDUCACIN CONTINUA POR LA DRA. VERNICA

    E-print Network

    Islas, León

    PUENTES ENTRE LA LITERATURA Y LAS ARTES PLÁSTICAS. CURSO DE EDUCACIÓN CONTINUA POR LA DRA. VERÓNICA, estudiantes y público en general interesado en realizar investigaciones interdisciplinarias. Curso de ocho

  12. Usos de espacios pblicos por nios 1 Referencias de la publicacin

    E-print Network

    Paris-Sud XI, Université de

    Usos de espacios públicos por niños 1 Referencias de la publicación Legendre, A., & Gómez Herrera, J. (2011). Diferencias interindividuales en el conocimiento y en el uso de los espacios públicos uso de los espacios públicos exteriores por niños Alain Legendre1 and José Gómez-Herrera2 1 Université

  13. Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB

    SciTech Connect

    Tanabe, Mikio; Nimigean, Crina M.; Iverson, T.M. (Weill-Med); (Vanderbilt)

    2010-06-25

    PorB is the second most prevalent outer membrane protein in Neisseria meningitidis. PorB is required for neisserial pathogenesis and can elicit a Toll-like receptor mediated host immune response. Here, the x-ray crystal structure of PorB has been determined to 2.3 {angstrom} resolution. Structural analysis and cocrystallization studies identify three putative solute translocation pathways through the channel pore: One pathway transports anions nonselectively, one transports cations nonselectively, and one facilitates the specific uptake of sugars. During infection, PorB likely binds host mitochondrial ATP, and cocrystallization with the ATP analog AMP-PNP suggests that binding of nucleotides regulates these translocation pathways both by partial occlusion of the pore and by restricting the motion of a putative voltage gating loop. PorB is located on the surface of N. meningitidis and can be recognized by receptors of the host innate immune system. Features of PorB suggest that Toll-like receptor mediated recognition outer membrane proteins may be initiated with a nonspecific electrostatic attraction.

  14. An investigation of exploitation versus exploration in GBEA optimization of PORS 15 and 16 Problems

    SciTech Connect

    Koch, Kaelynn

    2012-05-08

    It was hypothesized that the variations in time to solution are driven by the competing mechanisms of exploration and exploitation.This thesis explores this hypothesis by examining two contrasting problems that embody the hypothesized tradeoff between exploration and exploitation. Plus one recall store (PORS) is an optimization problem based on the idea of a simple calculator with four buttons: plus, one, store, and recall. Integer addition and store are classified as operations, and one and memory recall are classified as terminals. The goal is to arrange a fixed number of keystrokes in a way that maximizes the numerical result. PORS 15 (15 keystrokes) represents the subset of difficult PORS problems and PORS 16 (16 keystrokes) represents the subset of PORS problems that are easiest to optimize. The goal of this work is to examine the tradeoff between exploitation and exploration in graph based evolutionary algorithm (GBEA) optimization. To do this, computational experiments are used to examine how solutions evolve in PORS 15 and 16 problems when solved using GBEAs. The experiment is comprised of three components; the graphs and the population, the evolutionary algorithm rule set, and the example problems. The complete, hypercube, and cycle graphs were used for this experiment. A fixed population size was used.

  15. Evaluation of swabbing methods for estimating the prevalence of bacterial carriage in the upper respiratory tract: a cross sectional study

    PubMed Central

    Coughtrie, A L; Whittaker, R N; Begum, N; Anderson, R; Tuck, A; Faust, S N; Jefferies, J M; Yuen, H M; Roderick, P J; Mullee, M A; Moore, M V; Clarke, S C

    2014-01-01

    Objectives Bacterial carriage in the upper respiratory tract is usually asymptomatic but can lead to respiratory tract infection (RTI), meningitis and septicaemia. We aimed to provide a baseline measure of Streptococcus pneumoniae, Moraxella catarrhalis, Pseudomonas aeruginosa, Staphylococcus aureus, Haemophilus influenzae and Neisseria meningitidis carriage within the community. Self-swabbing and healthcare professional (HCP) swabbing were compared. Design Cross-sectional study. Setting Individuals registered at 20 general practitioner practices within the Wessex Primary Care Research Network South West, UK. Participants 10?448 individuals were invited to participate; 5394 within a self-swabbing group and 5054 within a HCP swabbing group. Self-swabbing invitees included 2405 individuals aged 0–4?years and 3349 individuals aged ?5?years. HCP swabbing invitees included 1908 individuals aged 0–4?years and 3146 individuals aged ?5?years. Results 1574 (15.1%) individuals participated, 1260 (23.4%, 95% CI 22.3% to 24.5%) undertaking self-swabbing and 314 (6.2%, 95% CI 5.5% to 6.9%) undertaking HCP-led swabbing. Participation was lower in young children and more deprived practice locations. Swab positivity rates were 34.8% (95% CI 32.2% to 37.4%) for self-taken nose swabs (NS), 19% (95% CI 16.8% to 21.2%) for self-taken whole mouth swabs (WMS), 25.2% (95% CI 20.4% to 30%) for nasopharyngeal swabs (NPS) and 33.4% (95% CI 28.2% to 38.6%) for HCP-taken WMS. Carriage rates of S. aureus were highest in NS (21.3%). S. pneumoniae carriage was highest in NS (11%) and NPS (7.4%). M. catarrhalis carriage was highest in HCP-taken WMS (28.8%). H. influenzae and P. aeruginosa carriage were similar between swab types. N. meningitidis was not detected in any swab. Age and recent RTI affected carriage of S. pneumoniae and H. influenzae. Participant costs were lower for self-swabbing (£41.21) versus HCP swabbing (£69.66). Conclusions Higher participation and lower costs of self-swabbing as well as sensitivity of self-swabbing favour this method for use in large population-based respiratory carriage studies. PMID:25358677

  16. Por Secretion System-Dependent Secretion and Glycosylation of Porphyromonas gingivalis Hemin-Binding Protein 35

    PubMed Central

    Shoji, Mikio; Sato, Keiko; Yukitake, Hideharu; Kondo, Yoshio; Narita, Yuka; Kadowaki, Tomoko; Naito, Mariko; Nakayama, Koji

    2011-01-01

    The anaerobic Gram-negative bacterium Porphyromonas gingivalis is a major pathogen in severe forms of periodontal disease and refractory periapical perodontitis. We have recently found that P. gingivalis has a novel secretion system named the Por secretion system (PorSS), which is responsible for secretion of major extracellular proteinases, Arg-gingipains (Rgps) and Lys-gingipain. These proteinases contain conserved C-terminal domains (CTDs) in their C-termini. Hemin-binding protein 35 (HBP35), which is one of the outer membrane proteins of P. gingivalis and contributes to its haem utilization, also contains a CTD, suggesting that HBP35 is translocated to the cell surface via the PorSS. In this study, immunoblot analysis of P. gingivalis mutants deficient in the PorSS or in the biosynthesis of anionic polysaccharide-lipopolysaccharide (A-LPS) revealed that HBP35 is translocated to the cell surface via the PorSS and is glycosylated with A-LPS. From deletion analysis with a GFP-CTD[HBP35] green fluorescent protein fusion, the C-terminal 22 amino acid residues of CTD[HBP35] were found to be required for cell surface translocation and glycosylation. The GFP-CTD fusion study also revealed that the CTDs of CPG70, peptidylarginine deiminase, P27 and RgpB play roles in PorSS-dependent translocation and glycosylation. However, CTD-region peptides were not found in samples of glycosylated HBP35 protein by peptide map fingerprinting analysis, and antibodies against CTD-regions peptides did not react with glycosylated HBP35 protein. These results suggest both that the CTD region functions as a recognition signal for the PorSS and that glycosylation of CTD proteins occurs after removal of the CTD region. Rabbits were used for making antisera against bacterial proteins in this study. PMID:21731719

  17. In silico studies of outer membrane of Neisseria meningitidis por a: its expression and immunogenic properties.

    PubMed

    Behrouzi, Ava; Bouzari, Saeid; Siadat, Seyed Davar; Irani, Shiva

    2014-01-01

    Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from serogroup B N. meningitidis was cloned into prokaryotic expression vector pBAD-gIIIA. Recombinant protein was expressed with arabinose and affinity purified by Ni-NTA agarose, SDS-PAGE and western blotting were performed for protein determination and verification. BALB/c mice were immunized subcutaneously with purified rPorA together with alum adjuvant. Serum antibody responses to serogroups B N.meningitidis were determined by ELISA. Serum IgG response significantly increased in the group immunized with rPorA together with alum adjuvant in comparison with control groups. These results suggest that rPorA can be a potential vaccine candidate for serogroup B N.meningitidis. PMID:25317403

  18. In Silico Studies of Outer Membrane of Neisseria Meningitidis Por A: Its Expression and Immunogenic Properties

    PubMed Central

    Behrouzi, Ava; Bouzari, Saeid; Siadat, Seyed Davar; Irani, Shiva

    2014-01-01

    Neisseria meningitidis is a major causative agent of bacterial septicemia and meningitis in humans. Currently, there are no vaccines to prevent disease caused by strains of N.meningitidis serogroup B. The Class 1 Outer Membrane Protein (OMP) has been named porA which is a cation selective transmembrane protein of 45 KDa that forms trimeric pore in the meningococcal outer membrane. PorA from serogroup B N. meningitidis was cloned into prokaryotic expression vector pBAD-gIIIA. Recombinant protein was expressed with arabinose and affinity purified by Ni-NTA agarose, SDS-PAGE and western blotting were performed for protein determination and verification. BALB/c mice were immunized subcutaneously with purified rPorA together with alum adjuvant. Serum antibody responses to serogroups B N.meningitidis were determined by ELISA. Serum IgG response significantly increased in the group immunized with rPorA together with alum adjuvant in comparison with control groups. These results suggest that rPorA can be a potential vaccine candidate for serogroup B N.meningitidis. PMID:25317403

  19. NMR localization of the O-mycoloylation on PorH, a channel forming peptide from Corynebacterium glutamicum.

    PubMed

    Rath, Parthasarathi; Saurel, Olivier; Tropis, Maryelle; Daffé, Mamadou; Demange, Pascal; Milon, Alain

    2013-11-15

    PorH and PorA are two small peptides that, in complex, form a voltage-dependent ion channel in the outer membrane of Corynebacterium glutamicum. Specific post-translational modifications on PorA and PorH are required for the formation of a functional ion channel. The assignment of PorH proton NMR chemical shifts in DMSO, allowed identifying unambiguously the exact position of the PorH O-mycoloylation on Ser 56 side chain. This was further confirmed by site directed mutagenesis and mass spectrometry. Together with the previously published localization of PorA mycoloylation, this provides the complete primary structure characterization of this outer membrane porin. PMID:24100136

  20. COMUNICA - PLATAFORMA PARA EL DESARROLLO, DISTRIBUCIÓN Y EVALUACIÓN DE HERRAMIENTAS LOGOPÉDICAS ASISTIDAS POR ORDENADOR

    Microsoft Academic Search

    Oscar Saz; Eduardo Lleida; Carlos Vaquero

    RESUMEN Este trabajo presenta Comunica, la plataforma desarrolla- da por el Instituto de Investigación en Ingeniería de Aragón (I3A) en colaboración con diversas entidades educativas para el desarrollo y distribución de diferentes herramien- tas de logopedia y enseñanza lingüística basadas en Tec- nologías del Habla. El conjunto de aplicaciones desarrol- ladas proporciona entrenamiento desde los niveles prel- ingüísticos a losniveles

  1. RODPROW Projeto Geomtrico de Vias Assistido por Computador REYNALDO COSATI MEDEIROS 1

    E-print Network

    plataforma da estrada (que passa a a ser vista como uma superfície com bordo). A plataforma fica definida, em quase todos os pontos por parâmetros pré­fixados (largura da plataforma, super­elevação de curvas da plataforma, é importante avaliar o volume de terra (corte ou aterro) na terraplenagem. Para tal, é

  2. RODPROW -Projeto Geomtrico de Vias Assistido por Computador REYNALDO COSATI MEDEIROS

    E-print Network

    plataforma da estrada (que passa a a ser vista como uma superfície com bordo). A plataforma fica definida, em quase todos os pontos por parâmetros pré-fixados (largura da plataforma, super-elevação de curvas da plataforma, é importante avaliar o volume de terra (corte ou aterro) na terraplenagem. Para tal, é

  3. Que pas este? Narrativas sobre o Brasil criadas por brasileiros residentes no exterior

    E-print Network

    Paris-Sud XI, Université de

    Que país é este? Narrativas sobre o Brasil criadas por brasileiros residentes no exterior Luis utilizados no Brasil, como o Orkut, existem mais de sessenta comunidades com o título "Brasileiros-representação e a imagem do Brasil e dos brasileiros no exterior ou sobre a percepção da terra de chegada. O

  4. Mortalidade precoce por doenças do coração no brasil. Comparação com outros países

    Microsoft Academic Search

    Paulo Andrade Lotufo

    1998-01-01

    Objetivo - Comparar a mortalidade por doenças do coração e pela doença coronária (DC) nas faixas etárias dos 45 aos 64 anos, no período 1984-87, em capitais bra- sileiras com outros países. Métodos - Foram utilizadas as estatísticas oficiais de mortalidade de 8 capitais com boa qualidade de informa- ção: Belém, Recife, Salvador, Belo Horizonte, Rio de Ja- neiro, São

  5. Prevalencia y tamizaje del Trastorno por Déficit de Atención con Hiperactividad en Costa Rica

    PubMed Central

    Weiss, Nicholas T.; Schuler, Jovita; Monge, Silvia; McGough, James J.; Chavira, Denise; Bagnarello, Monica; Herrera, Luis Diego; Mathews, Carol A.

    2015-01-01

    Resumen La investigación tuvo como propósito estimar la prevalencia del Trastorno por Déficit de Atención con Hiperactividad (TDAH) en Costa Rica y determinar si la versión en español del cuestionario Swanson Nolan and Pelham Scale IV (SNAP-IV) es un instrumento de tamizaje útil en una población de niños y niñas escolares costarricenses. El instrumento fue entregado a padres y maestros de 425 niños entre 5 y 13 años de edad (promedio = 8.8). Todos fueron evaluados con el instrumento Swanson, Kotkin, Agler, M-Flynn and Pelham Scale (SKAMP). Su diagnóstico fue confirmado con una entrevista clínica. La sensibilidad y la especificidad del SNAP-IV fueron evaluadas como predictores de criterios de diagnóstico según el DSM-IV. La prevalencia puntual en la muestra del TDAH fue del 5%. El tamizaje más preciso lo hizo el SNAP-IV completado por el maestro en un corte de 20%, con una sensibilidad de 96% y una especificidad de un 82%. La sensibilidad de los instrumentos completados por los padres fue más baja que aquella de los maestros. El SNAP-IV completado por las maestras con un corte aislando el 20% de los mayores puntajes categorizó correctamente a un 87% de los sujetos. PMID:22432094

  6. Programacion Funcional Curso en la Maestra en Ciencias de la Computacion, UNAM. Por Carlos Velarde.

    E-print Network

    Velarde, Carlos

    Programaciâ??on Funcional Curso en la Maestrâ??�a en Ciencias de la Computaciâ??on, UNAM. Por Carlosâ??on funcional. Introducciâ??on En la programaciâ??on funcional los programas se componen de definiciones de funciones matemâ??aticas mediante ecuaciones, la computaciâ??on corresponde a la aplicaciâ??on de funciones

  7. Gesto por polticas: arquitectura e aplicaes Filipe Caldeira1,2

    E-print Network

    Monteiro, Edmundo

    ://lct.dei.uc.pt Palavras chave: PBN, Segurança de redes, Gestão de redes por políticas, COPS, COPS-PR, SPSL. Resumo Neste artigo é feita uma abordagem à gestão de redes baseadas em políticas focando a arquitectura PBN (Policy aplicabilidade na arquitectura PBN. No âmbito dos protocolos de difusão de política, destaca- se o protocolo COPS

  8. ANLISE DE SUPERFCIES BOMBARDEADAS POR FEIXES DE ELTRONS AUTOFOCALIZADOS EM UM SISTEMA DE PLAMA

    E-print Network

    ANÁLISE DE SUPERFÍCIES BOMBARDEADAS POR FEIXES DE ELÉTRONS AUTOFOCALIZADOS EM UM SISTEMA DE PLAMA/LAP/INPE, Orientador) RESUMO Este trabalho, iniciado em agosto de 2004, tem como objetivo a continuidade ao projeto de Iniciação Científica em andamento desde 2003, para a aplicação de pulsos rápidos de calor resultantes de

  9. INFLUÊNCIA DE DIFERENTES CLASSES DE INFESTAÇÃO POR MANCHA PÚRPURA SOBRE O VIGOR DE SEMENTES DE SOJA

    Microsoft Academic Search

    LUCIANO DOS; REIS VENTUROSO; MARCO ANTÔNIO SEDREZ; FÁBIO RÉGIS DE SOUZA; ANDERSON CRISTIAN BERGAMIN; LENITA APARECIDA CONUS

    RESUMO: O trabalho propôs-se a analisar a influência de diferentes classes de infestação por mancha púrpura (Cercospora kikuchii) no tegumento das sementes de três cultivares de soja, sobre o vigor de plântulas no município de Dourados - MS. O experimento foi conduzido na Embrapa Agropecuária Oeste, em outubro de 2006. As classes de infestação foram determinadas visualmente e classificadas em

  10. Chris Quigg Las revoluciones por venir en Fsica de Partculas Museo de la Ciencia Chris Quigg

    E-print Network

    Quigg, Chris

    Chris Quigg · Las revoluciones por venir en Física de Partículas · Museo de la Ciencia Chris Quigg Ciencias Fisicas no nostenga reservadas maravillas aún más impresionantes que aquellas del pasado, parece Ciencias Físicas deberán ser buscadas en las sextas cifras decimales." Tres revoluciones de Galileo Eppur

  11. Padrão da prescrição de atividade física realizada por médicos ortopedistas brasileiros

    Microsoft Academic Search

    Alexandre Mader Seixas; Sandra M. M. Matsudo; Victor K. R. Matsudo; Erinaldo L. Andrade; Glaucia F. Braggion

    Objetivo: avaliar a prescrição de atividade física realizada por ortopedistas brasileiros. Metodologia: 521 ortopedistas responderam um questionário sobre prescrição de atividade física. Os parâmetros prescritos de freqüência, duração e intensidade considerados corretos foram: realizar pelo menos 30 minutos contínuos ou acumulados de atividade moderada na maioria dos dias da semana. Utilizada análise estatística descritiva. Resultados: 86,4% prescrevem atividade física para

  12. CIENCIAS 99 JULIO SEPTIEMBRE 2010 La opresin colonial, las luchas por la libertad y contra las

    E-print Network

    Islas, León

    26 CIENCIAS 99 JULIO SEPTIEMBRE 2010 La opresión colonial, las luchas por la libertad y contra las enfermedades y las epidemias podrían describirse como historias paralelas, que en determinadas condiciones se entrecruzan. Manuela Sáenz es quizás el arquetipo de la heroína de la independencia y autodeterminación de los

  13. ¿POR QUE COMENZAR CON UN INGRESO CIUDADANO PARA LOS MENORES EN AMERICA LATINA?

    Microsoft Academic Search

    Alberto C. Barbeito; Rubén M. Lo Vuolo

    La característica central de todas las nociones afines con el concepto de ingreso básico o ingreso ciudadano (IC) es que se refieren a un arreglo institucional que garantiza cierta forma de ingreso incondicional a las personas, esto es, un ingreso para cuyo acceso no se requiere ninguna otra condición personal que la de ser ciudadano. Por ejemplo, no se requiere

  14. Violencia y prosocialidad en los contenidos televisivos infantiles visionados por menores

    E-print Network

    Escolano, Francisco

    Violencia y prosocialidad en los contenidos televisivos infantiles visionados por menores en no desaparecen. Los menores tienden a visionar más horas los programas de adultos que los específicamente. · La influencia en la conducta del niño es inversamente proporcional a la edad del mismo. Se aprecia

  15. Trastorno por déficit de atención con hiperactividad: orientaciones psicoeducativas para los padres

    Microsoft Academic Search

    López Villalobos; S. Alberola López; José Antonio López Villalobos

    2008-01-01

    Resumen Se exponen orientaciones psicoeducativas para padres de niños con trastorno por déficit de atención con hiperactividad (TDAH), basadas en criterios empíricos, teóricos y clínicos, abor- dando las siguientes dimensiones: ambiente familiar, modelado, refuerzo de conducta, normas, autonomía y autoestima, aprender a pensar y saber esperar, atención, relación con compañeros, grupo familiar, adolescencia y una reflexión sobre el tratamiento. Palabras

  16. Listado de cursos por temtica Artes plsticas y visuales en Alicante (1960-2013)

    E-print Network

    Escolano, Francisco

    Listado de cursos por temática ARTES Artes plásticas y visuales en Alicante (1960-2013) Curso internacional de cuerda y piano de la Universidad de Alicante (modalidad activo) Curso internacional de cuerda y de banda (modalidad oyente) VII Curso Internacional de Música Moderna (pop, rock, blues, jazz

  17. Listado de cursos de verano por lugar de realizacin CAMPUS DE LA UA

    E-print Network

    Escolano, Francisco

    Listado de cursos de verano por lugar de realización CAMPUS DE LA UA Economía del bien común Propiedad horizontal y su problemática actual Curso Rromipen/gitanidad: historia y cultura del pueblo gitano nuevas técnicas Curso de rescate acuático avanzado Derecho privado e Internet Literatura infantil y

  18. Otras designaciones Distribucin por empleos presupuestarios al 31/12/05

    E-print Network

    @ @ @ @ @ @ @ @ @ @ @ @ @ @ @ IRD centros y representaciones 1-3 4-6 7-12 13-25 26-50 51-62 105 133 @ Bélgica Marruecos El IRD en el representación en Marruecos. Además, el IRD también pugna por impulsar las capacidades de investigación de la

  19. FUNDAMENTACIN El hip hop es un movimiento artstico compuesto por un amplio conglomerado de

    E-print Network

    Escolano, Francisco

    HIP HOP FUNDAMENTACIÓN El hip hop es un movimiento artístico compuesto por un amplio conglomerado las áreas urbanas de escasos recursos de Nueva York, así que el hip hop funcionó, inicialmente, como. OBJETIVOS Los objetivos que se plantean con la práctica de hip hop son: Mejorar la condición física general

  20. LA UA EN CIFRAS Evolucin series histricas: Evolucin PDI por Categoras

    E-print Network

    Escolano, Francisco

    LA UA EN CIFRAS Evolución series históricas: Evolución PDI por Categorías Año 2011 Total % Mujer FORMACION (C-VALI) 15 0,6% 9 1,0% 6 0,4% INVESTIGADOR PDI 21 0,9% 8 0,9% 13 0,8% PROFESOR ASOCIADO 71 2

  1. LA UA EN CIFRAS Evolucin series histricas: Evolucin PDI por Categoras

    E-print Network

    Escolano, Francisco

    LA UA EN CIFRAS Evolución series históricas: Evolución PDI por Categorías Año 2009 Mujer % Hombre,13% 4 0,17% INVESTIGADOR PDI 5 0,61% 19 1,23% 24 1,01% PROFESOR ASOCIADO 88 10,69% 260 16,82% 348 14

  2. Trastorno por déficit de atención\\/hiperactividad: estado de la cuestión y futuras líneas de investigación

    Microsoft Academic Search

    E. Cardo; M. Servera

    2008-01-01

    ('inatento', 'hiperactivo\\/impulsivo', 'combinado') arroja más con- fusión, especialmente el subtipo inatento, que a menudo no con- cuerda con el conocimiento general que tienen los profesionales sobre el TDAH. Existe, por tanto, una gran controversia en la misma comuni- dad científica sobre las dimensiones reales de este trastorno, que aún hoy en día continúa sin ninguna prueba diagnóstica patogno- mónica y

  3. INFORME DE UN CUESTIONARIO ACERCA DE LAS REACCIONES EXPERIMENTADAS POR UN SANADOR BRASILERO

    Microsoft Academic Search

    Stanley Krippner

    Resúmen.- Diez personas quienes visitaron a un sanador filipino durante un viaje de estudios a Brasil completaron un cuestionario poco tiempo después de sus sesiones de sanación luego lo hicieron 6 y 12 meses más tarde. Los cuestionarios fueron diseñados para poder conocer algunas de las actitudes y reacciones experimentadas por estos sujetos. Un análisis estadístico de las respuestas determinó

  4. Estimacin de la Recarga por Retorno de Riego a partir de la

    E-print Network

    Politècnica de Catalunya, Universitat

    controlado por p y g · k suelo no saturado es función o p Considera ( ) ( ) ( ) ( ) 1w H h z h J K h k h K h Richards Planta-Modelo Feddes Parámetros no saturados-Modelo van Genuchten Solución Inversa ROSETTA (FETs Joaquín Jiménez CONCEPTOS PREVIOS : succión ( = -h) Richards (1931) C(): capacidad específica (pendt

  5. Memorias Tercer Congreso Internacional sobre la Enseñanza de la Matemática Asistida por Computadora

    Microsoft Academic Search

    Lorena Acuña; Maricella Becerril; Elsie Villalobos

    Un ambiente de aprendizaje apoyado con computadoras bajo un enfoque algorítmico, por medio de presentaciones de PowerPoint, puede resultar de gran ayuda para la en señanza de la Matemática. Dentro del proceso de ap rendizaje, en la fase de motivación puede resultar altamente estimulante a n ivel sensorial; en la fase de comprensión permite l a reiteración de los conceptos

  6. INFLUÊNCIA DE TRATAMENTOS TÉRMICOS NO NÍVEL DE TENSÕES RESIDUAIS EM RECOBRIMENTOS PROCESSADOS POR ASPERSÃO TÉRMICA

    Microsoft Academic Search

    C. Godoy; E. A. Souza; J. R. T. Branco; M. M. Lima

    RESUMO A deposição por aspersão térmica de recobrimentos com coeficiente de dilatação maior que o substrato introduz tensões residuais trativas no recobrimento. Isso devie-sie ao resfriamento das partículas depositadas sobrie um substrato a uma temperatura Ts e devido ao resfriamento do conjugado, da temperatura Ts até a temperatura ambiente. Recobrimentos mais espessos apresentam tensões residuais de compressão na superfície e

  7. Departamento de Cincia dos Materiais e Metalurgia Caracterizao de Tubos Compsitos por Microscopia Digital

    E-print Network

    camadas de fibras banhadas em resina são enroladas em torno de um mandril para gerar uma peça com simetria (roving) passam por um banho de resina e são enroladas com ângulos pré-determinados sobre um mandril diversas etapas para a obtenção de um produto final de alta qualidade: · Após conferir o estado do mandril

  8. Sepsis por Salmonella enteritidis en una paciente con lupus eritematoso sistémico

    Microsoft Academic Search

    Carlos Alberto Cañas; Gustavo Adolfo Ospina; María Elena Ochoa; Juan Diego Vélez

    Resumen Presentamos el caso clínico de una paciente de 28 años de edad con Lupus Eritematoso Sistémico (LES), con compromiso a nivel neurológico central, riñón y sistema hematológico, con tratamiento cró- nico de esteroides y desnutrición avanzada, quien desarrolla sepsis por Salmonella enteritidis, germen aislado en muestras tomadas en lavado bronco alveolar, sangre y materia fecal. Presentó un absce- so

  9. Ceftaroline potency among 9 US Census regions: report from the 2010 AWARE Program.

    PubMed

    Flamm, Robert K; Sader, Helio S; Farrell, David J; Jones, Ronald N

    2012-09-01

    Ceftaroline is a new antibacterial agent that is active against the major bacterial pathogens found in acute bacterial skin and skin structure infections and community-acquired bacterial pneumonia. The 2010 Assessing Worldwide Antimicrobial Resistance Evaluation (AWARE) Surveillance Program in the United States collected a total of 8434 bacterial isolates from 65 US medical centers across 9 US regions. The isolates were cultured and tested for susceptibility to ceftaroline and comparator agents by reference minimum inhibitory concentration (MIC) methods. An analysis by US Census Bureau region demonstrated that Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), and coagulase-negative staphylococci (CoNS), including methicillin-resistant CoNS, were particularly susceptible to ceftaroline (MIC(90), 1 and 0.5 µg/mL respectively). The MRSA rate was 50.0% overall, which varied from a low of 44.6% in the South Atlantic region to a high of 53.1% in the Mountain region. Susceptibility among MRSA for ceftaroline ranged from 96.7% in the West South Central region to 100% in the West North Central region. All MRSA isolates were inhibited at a ceftaroline MIC of ? 2 ?g/mL, and 98.4% were inhibited at a ceftaroline MIC of ? 1 ?g/mL. In general, regional differences in activity among staphylococci, streptococci, Haemophilus spp., and Moraxella catarrhalis were minimal due to the high potency of ceftaroline. Greater differences in activity were observed among the Enterobacteriaceae due to the greater diversity of organism types and resistance mechanisms, including those producing extended-spectrum ?-lactamase enzymes. PMID:22903952

  10. Airway bacteria measured by quantitative polymerase chain reaction and culture in patients with stable COPD: relationship with neutrophilic airway inflammation, exacerbation frequency, and lung function

    PubMed Central

    Bafadhel, Mona; Haldar, Koirobi; Barker, Bethan; Patel, Hemu; Mistry, Vijay; Barer, Michael R; Pavord, Ian D; Brightling, Christopher E

    2015-01-01

    Background Potentially pathogenic microorganisms can be detected by quantitative real-time polymerase chain reaction (qPCR) in sputum from patients with COPD, although how this technique relates to culture and clinical measures of disease is unclear. We used cross-sectional and longitudinal data to test the hypotheses that qPCR is a more sensitive measure of bacterial presence and is associated with neutrophilic airway inflammation and adverse clinical outcomes. Methods Sputum was collected from 174 stable COPD subjects longitudinally over 12 months. Microbial sampling using culture and qPCR was performed. Spirometry and sputum measures of airway inflammation were assessed. Findings Sputum was qPCR-positive (>106 copies/mL) in 77/152 samples (Haemophilus influenzae [n=52], Moraxella catarrhalis [n=24], Streptococcus pneumoniae [n=19], and Staphylococcus aureus [n=7]). Sputum was culture-positive in 50/174 samples, with 49 out of 50 culture-positive samples having pathogen-specific qPCR bacterial loads >106 copies/mL. Samples that had qPCR copy numbers >106/mL, whether culture-positive or not, had increased sputum neutrophil counts. H. influenzae qPCR copy numbers correlated with sputum neutrophil counts (r=0.37, P<0.001), were repeatable within subjects, and were >106/mL three or more times in 19 patients, eight of whom were repeatedly sputum culture-positive. Persistence, whether defined by culture, qPCR, or both, was associated with a higher sputum neutrophil count, lower forced expiratory volume in 1 second (FEV1), and worsened quality of life. Interpretation qPCR identifies a significant number of patients with potentially bacteria-associated neutrophilic airway inflammation and disease that are not identified by traditional culture-based methods.

  11. Nationwide surveillance of 6 otorhinolaryngological infectious diseases and antimicrobial susceptibility pattern in the isolated pathogens in Japan.

    PubMed

    Suzuki, Kenji; Kurono, Yuichi; Ikeda, Katsuhisa; Watanabe, Akira; Iwamoto, Aikichi; Totsuka, Kyoichi; Kaku, Mitsuo; Iwata, Satoshi; Kadota, Jun-Ichi; Hanaki, Hideaki

    2015-07-01

    The Japanese Three Academic Societies Joint Antimicrobial Susceptibility Surveillance Committee has conducted a nationwide surveillance on antimicrobial susceptibility patterns and rates of isolation in 6 otolaryngological diseases. The surveillance program was conducted in the otorhinolaryngological departments of 29 universities, and their 26 affiliated hospitals. Patients suffering from acute otitis media, chronic otitis media, acute nasal sinusitis, chronic nasal sinusitis, acute tonsillitis, and peritonsillar abscess between January 2011 and June 2012 were investigated. The collected swab or incision samples were cultivated for microbial identification, and the drug susceptibility of detected bacteria was measured at the Kitasato University Research Center for Infections and Antimicrobials. The surveillance focused on three gram-positive bacteria (Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus), three gram-negative bacteria (Haemophilus influenzae, Moraxella Catarrhalis, and Pseudomonas aeruginosa), and three anaerobic bacteria (Peptostreptococcus spp., Prevotella spp., and Fusobacterium spp.). Bacterial susceptibility to 39 antimicrobial drugs was investigated. We compared bacterial isolation ratio of each disease in this surveillance from those of past 4 times surveillance which we performed formerly, and we also compared percentage of main drug resistant strains from those of past 4 times surveillance. The age composition between this time and former surveillances was not statistically significant by student-t test. We were unable to completely resolve the rise in resistant bacteria, such as methicillin-resistant S. aureus, penicillin-resistant S. pneumoniae, penicillin-intermediate resistant S. pneumoniae, beta-lactamase non-producing ampicillin-resistant H. influenzae, beta-lactamase producing ampicillin-resistant H. influenzae, and beta-lactamase producing amoxicillin clavulanic acid-resistant H. influenzae. We suggest promoting the proper usage of antimicrobial drugs in order to avoid the spread of these bacteria. PMID:26004175

  12. Co-colonization by Haemophilus influenzae with Streptococcus pneumoniae enhances pneumococcal-specific antibody response in young children

    PubMed Central

    Xu, Qingfu; Pichichero, Michael E.

    2015-01-01

    Background Streptococcus pneumoniae (Spn), Haemophilus influenzae (Hi) and Moraxella catarrhalis (Mcat) are common bacterial pathogens of respiratory infections and common commensal microbes in the human nasopharynx (NP). The effect of interactions among theses bacteria during co-colonization of the NP on the host immune response has not been evaluated. The objective of this study was to assess the impact of co-colonization by Hi or Mcat on the systemic antibody response to vaccine protein candidate antigens of Spn and similarly the impact of co-colonization by Spn and Mcat on antibody responses to Hi vaccine protein candidate antigens. Methods Serum samples were collected from healthy children at 6, 9, 15, 18, and 24 months of age when they were colonized with Spn, Hi, Mcat or their combinations. Quantitative ELISA was used to determine serum IgA and IgG against three Spn antigens and three Hi antigens, and as well as whole cells of non-typeable (NT) Spn and Hi. Results NP colonization by Spn increased serum IgA and IgG titers against Spn antigens PhtD, PcpA and PlyD and whole cells of NTSpn, and co-colonization of Hi or Mcat with Spn resulted in further increases of serum pneumococcal-specific antibody levels. NP colonization by Hi increased serum IgA and IgG titers against Hi antigens P6, Protein D and OMP26 and whole cells of NTHi, but co-colonization of Spn or Mcat with Hi did not result in further increase of serum NTHi-specific antibody levels. Conclusion Co-colonization of Hi or Mcat with Spn enhances serum antibody response to NTSpn whole cells and Spn vaccine candidate antigens PhtD, PcPA and PlyD1. Co-colonization appears to variably modulate pathogen species-specific host adaptive immune response. PMID:24355091

  13. In Vitro and In Vivo Antibacterial Activities of Heteroaryl Isothiazolones against Resistant Gram-Positive Pathogens?

    PubMed Central

    Pucci, Michael J.; Cheng, Jijun; Podos, Steven D.; Thoma, Christy L.; Thanassi, Jane A.; Buechter, Douglas D.; Mushtaq, Gohar; Vigliotti, Gerald A.; Bradbury, Barton J.; Deshpande, Milind

    2007-01-01

    The activities of several tricyclic heteroaryl isothiazolones (HITZs) against an assortment of gram-positive and gram-negative clinical isolates were assessed. These compounds target bacterial DNA replication and were found to possess broad-spectrum activities especially against gram-positive strains, including antibiotic-resistant staphylococci and streptococci. These included methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-nonsusceptible staphylococci, and quinolone-resistant strains. The HITZs were more active than the comparator antimicrobials in most cases. For gram-negative bacteria, the tested compounds were less active against members of the family Enterobacteriaceae but showed exceptional potencies against Haemophilus influenzae, Moraxella catarrhalis, and Neisseria spp. Good activity against several anaerobes, as well as Legionella pneumophila and Mycoplasma pneumoniae, was also observed. Excellent bactericidal activity against staphylococci was observed in time-kill assays, with an approximately 3-log drop in the numbers of CFU/ml occurring after 4 h of exposure to compound. Postantibiotic effects (PAEs) of 2.0 and 1.7 h for methicillin-susceptible S. aureus and MRSA strains, respectively, were observed, and these were similar to those seen with moxifloxacin at 10× MIC. In vivo efficacy was demonstrated in murine infections by using sepsis and thigh infection models. The 50% protective doses were ?1 mg/kg of body weight against S. aureus in the sepsis model, while decreases in the numbers of CFU per thigh equal to or greater than those detected in animals treated with a standard dose of vancomycin were seen in the animals with thigh infections. Pharmacokinetic analyses of treated mice indicated exposures similar to those to ciprofloxacin at equivalent dose levels. These promising initial data suggest further study on the use of the HITZs as antibacterial agents. PMID:17242152

  14. Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections.

    PubMed

    Gadsby, N J; McHugh, M P; Russell, C D; Mark, H; Conway Morris, A; Laurenson, I F; Hill, A T; Templeton, K E

    2015-08-01

    The frequent lack of a positive and timely microbiological diagnosis in patients with lower respiratory tract infection (LRTI) is an important obstacle to antimicrobial stewardship. Patients are typically prescribed broad-spectrum empirical antibiotics while microbiology results are awaited, but, because these are often slow, negative, or inconclusive, de-escalation to narrow-spectrum agents rarely occurs in clinical practice. The aim of this study was to develop and evaluate two multiplex real-time PCR assays for the sensitive detection and accurate quantification of Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Moraxella catarrhalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. We found that all eight bacterial targets could be reliably quantified from sputum specimens down to a concentration of 100 CFUs/reaction (8333 CFUs/mL). Furthermore, all 249 positive control isolates were correctly detected with our assay, demonstrating effectiveness on both reference strains and local clinical isolates. The specificity was 98% on a panel of nearly 100 negative control isolates. Bacterial load was quantified accurately when three bacterial targets were present in mixtures of varying concentrations, mimicking likely clinical scenarios in LRTI. Concordance with culture was 100% for culture-positive sputum specimens, and 90% for bronchoalveolar lavage fluid specimens, and additional culture-negative bacterial infections were detected and quantified. In conclusion, a quantitative molecular test for eight key bacterial causes of LRTI has the potential to provide a more sensitive decision-making tool, closer to the time-point of patient admission than current standard methods. This should facilitate de-escalation from broad-spectrum to narrow-spectrum antibiotics, substantially improving patient management and supporting efforts to curtail inappropriate antibiotic use. PMID:25980353

  15. Recurrent, protracted and persistent lower respiratory tract infection: A neglected clinical entity.

    PubMed

    Verhagen, Lilly M; de Groot, Ronald

    2015-06-01

    Community-acquired pneumonia is a potentially life-threatening disease affecting children worldwide. Recurrent pneumonia episodes can lead to the development of chronic respiratory morbidity. Chronic wet cough, a common pediatric complaint, is defined as a wet cough indicating excessive airway mucus that lasts for a minimum of 4 weeks. Most children with a chronic wet cough do not suffer from underlying debilitating pulmonary disorders. Rather, chronic wet cough is generally associated with neutrophilic airway inflammation and bacterial infections of the conducting airways. Failure to characterize endobronchial infections has led to under-recognition of chronic wet cough as an important clinical entity in children. Under-recognition and under-treatment of protracted bacterial bronchitis (PBB), a diagnosis made by the presence of isolated cough >4 weeks that resolves with appropriate antibiotic treatment, may lead to the development of chronic suppurative lung disease (CSLD) and bronchiectasis. The burden of bronchiectasis is highest in developing countries and in specifically vulnerable populations in developed countries, in particular indigenous children living in remote communities. The incidence, hospitalization rates and risk of long term sequelae of childhood pneumonia in indigenous children are higher than in non-indigenous children residing in the same area. The overlapping clinical and pathophysiological characteristics of PBB, CSLD and bronchiectasis are the presence of a chronic wet cough, impaired mucociliary clearance of the conducting airways, the presence of endobronchial bacterial infection (mainly non-typeable Haemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis) and neutrophilic airway inflammation. The principles of managing PBB, CSLD and bronchiectasis are the same. More research and public health interventions are required to improve the awareness, diagnosis and management of these causes of chronic wet cough in children. PMID:25917807

  16. Double-blind, comparative study of rufloxacin once daily versus amoxicillin three times a day in treatment of outpatients with exacerbations of chronic bronchitis.

    PubMed Central

    Klietmann, W; Cesana, M; Rondel, R K; Focht, J

    1993-01-01

    In a double-blind, randomized, multicenter study, the efficacy and safety of two dosage schedules of rufloxacin once daily were compared with those of amoxicillin three times a day in the treatment of 192 outpatients with exacerbations of chronic bronchitis. Rufloxacin was given as a single oral dose of 400 mg on day 1 and single daily doses of 200 mg on the subsequent 9 days (n = 64) or as 300 mg on day 1 and then 150 mg daily for 9 days (n = 63); amoxicillin was given as 500 mg orally three times a day for 10 days (n = 65). Clinical and bacteriological assessments were carried out before treatment, between study days 3 and 5, and at days 1 and 8 after treatment. Pretreatment cultures were positive for 139 patients, the most frequently isolated pathogens being Streptococcus pneumoniae, Moraxella catarrhalis, and Haemophilus influenzae. Clinical success rates were comparable in the three groups (94, 95, and 98%, respectively), as were bacteriological success rates at the end of treatment (93, 95, and 91%, respectively) and at follow-up (88, 95, and 98%, respectively). The power to detect a significant 15% difference in cure rates was 74.9%. Follow-up bacteriological failures from pneumococcal infection were 18% in both rufloxacin groups combined and 5% in the amoxicillin group. The 200-mg dose regimen achieved average steady-state concentrations in plasma higher than did the 150-mg dose regimen (3.75 versus 2.72 micrograms/ml). Adverse events occurred in 11 and 13 patients, respectively, on rufloxacin and 8 on amoxicillin. This study shows that rufloxacin once daily ay be a possible option for the treatment of acute exacerbations of chronic bronchitis. The 200-mg daily oral dose preceeded by a loading dose of 400 mg displays a better pharmacokinetic profile than the lower dose. PMID:8285609

  17. Metabolic markers and microecological characteristics of tongue coating in patients with chronic gastritis

    PubMed Central

    2013-01-01

    Background In Traditional Chinese Medicine (TCM), tongue diagnosis has been an important diagnostic method for the last 3000 years. Tongue diagnosis is a non-invasive, simple and valuable diagnostic tool. TCM treats the tongue coating on a very sensitive scale that reflects physiological and pathological changes in the organs, especially the spleen and stomach. Tongue coating can diagnose disease severity and determine the TCM syndrome (“Zheng” in Chinese). The biological bases of different tongue coating appearances are still poorly understood and lack systematic investigation at the molecular level. Methods Tongue coating samples were collected from 70 chronic gastritis patients and 20 normal controls. 16S rRNA denatured gradient gel electrophoresis (16S rRNA–DGGE) and liquid chromatography and mass spectrometry (LC–MS) were designed to profile tongue coatings. The statistical techniques used were principal component analysis and partial least squares–discriminate analysis. Results Ten potential metabolites or markers were found in chronic gastritis patients, including UDP-D-galactose, 3-ketolactose, and vitamin D2, based on LC–MS. Eight significantly different strips were observed in samples from chronic gastritis patients based on 16S rRNA–DGGE. Two strips, Strips 8 and 10, were selected for gene sequencing. Strip 10 sequencing showed a 100% similarity to Rothia mucilaginosa. Strip 8 sequencing showed a 96.2% similarity to Moraxella catarrhalis. Conclusions Changes in glucose metabolism could possibly form the basis of tongue coating conformation in chronic gastritis patients. The study revealed important connections between metabolic components, microecological components and tongue coating in chronic gastritis patients. Compared with other diagnostic regimens, such as blood tests or tissue biopsies, tongue coating is more amenable to, and more convenient for, both patients and doctors. PMID:24041039

  18. In Vitro and In Vivo Profiles of ACH-702, an Isothiazoloquinolone, against Bacterial Pathogens?

    PubMed Central

    Pucci, Michael J.; Podos, Steven D.; Thanassi, Jane A.; Leggio, Melissa J.; Bradbury, Barton J.; Deshpande, Milind

    2011-01-01

    ACH-702, a novel isothiazoloquinolone (ITQ), was assessed for antibacterial activity against a panel of Gram-positive and Gram-negative clinical isolates and found to possess broad-spectrum activity, especially against antibiotic-resistant Gram-positive strains, including methicillin-resistant Staphylococcus aureus (MRSA). For Gram-negative bacteria, ACH-702 showed exceptional potency against Haemophilus influenzae, Moraxella catarrhalis, and a Neisseria sp. but was less active against members of the Enterobacteriaceae. Good antibacterial activity was also evident against several anaerobes as well as Legionella pneumophila and Mycoplasma pneumoniae. Excellent bactericidal activity was observed for ACH-702 against several bacterial pathogens in time-kill assays, and postantibiotic effects (PAEs) of >1 h were evident with both laboratory and clinical strains of staphylococci at 10× MIC and similar in most cases to those observed for moxifloxacin at the same MIC multiple. In vivo efficacy was demonstrated against S. aureus with murine sepsis and thigh infection models, with decreases in the number of CFU/thigh equal to or greater than those observed after vancomycin treatment. Macromolecular synthesis assays showed specific dose-dependent inhibition of DNA replication in staphylococci, and biochemical analyses indicated potent dual inhibition of two essential DNA replication enzymes: DNA gyrase and topoisomerase IV. Additional biological data in support of an effective dual targeting mechanism of action include the following: low MIC values (?0.25 ?g/ml) against staphylococcal strains with single mutations in both gyrA and grlA (parC), retention of good antibacterial activity (MICs of ?0.5 ?g/ml) against staphylococcal strains with two mutations in both gyrA and grlA, and low frequencies for the selection of higher-level resistance (<10?10). These promising initial data support further study of isothiazoloquinolones as potential clinical candidates. PMID:21464250

  19. In vitro and in vivo profiles of ACH-702, an isothiazoloquinolone, against bacterial pathogens.

    PubMed

    Pucci, Michael J; Podos, Steven D; Thanassi, Jane A; Leggio, Melissa J; Bradbury, Barton J; Deshpande, Milind

    2011-06-01

    ACH-702, a novel isothiazoloquinolone (ITQ), was assessed for antibacterial activity against a panel of Gram-positive and Gram-negative clinical isolates and found to possess broad-spectrum activity, especially against antibiotic-resistant Gram-positive strains, including methicillin-resistant Staphylococcus aureus (MRSA). For Gram-negative bacteria, ACH-702 showed exceptional potency against Haemophilus influenzae, Moraxella catarrhalis, and a Neisseria sp. but was less active against members of the Enterobacteriaceae. Good antibacterial activity was also evident against several anaerobes as well as Legionella pneumophila and Mycoplasma pneumoniae. Excellent bactericidal activity was observed for ACH-702 against several bacterial pathogens in time-kill assays, and postantibiotic effects (PAEs) of >1 h were evident with both laboratory and clinical strains of staphylococci at 10 × MIC and similar in most cases to those observed for moxifloxacin at the same MIC multiple. In vivo efficacy was demonstrated against S. aureus with murine sepsis and thigh infection models, with decreases in the number of CFU/thigh equal to or greater than those observed after vancomycin treatment. Macromolecular synthesis assays showed specific dose-dependent inhibition of DNA replication in staphylococci, and biochemical analyses indicated potent dual inhibition of two essential DNA replication enzymes: DNA gyrase and topoisomerase IV. Additional biological data in support of an effective dual targeting mechanism of action include the following: low MIC values (?0.25 ?g/ml) against staphylococcal strains with single mutations in both gyrA and grlA (parC), retention of good antibacterial activity (MICs of ?0.5 ?g/ml) against staphylococcal strains with two mutations in both gyrA and grlA, and low frequencies for the selection of higher-level resistance (<10?¹?). These promising initial data support further study of isothiazoloquinolones as potential clinical candidates. PMID:21464250

  20. PorA Represents the Major Cell Wall Channel of the Gram-Positive Bacterium Corynebacterium glutamicum

    Microsoft Academic Search

    Noelia Costa-Riu; Andreas Burkovski; Reinhard Kramer; Roland Benz

    2003-01-01

    The cell wall of the gram-positive bacterium Corynebacterium glutamicum contains a channel (porin) for the passage of hydrophilic solutes. The channel-forming polypeptide PorA is a 45-amino-acid acidic polypeptide with an excess of four negatively charged amino acids, which is encoded by the 138-bp gene porA. porA was deleted from the chromosome of C.glutamicum wild-type strain ATCC 13032 to obtain mutant

  1. Verification of a topology model of PorT as an integral outer membrane protein in Porphyromonas gingivalis

    PubMed Central

    Nguyen, Ky-Anh; ?ylicz, Jasiek; Szczesny, Pawel; Sroka, Aneta; Hunter, Neil; Potempa, Jan

    2009-01-01

    PorT is a membrane-associated protein shown to be essential for the maturation and secretion of a class of cysteine proteinases, the gingipains, from the periodontal pathogen Porphyromonas gingivalis. It was previously reported that PorT is located on the periplasmic surface of the inner membrane to function as a chaperone for the maturing proteinases. Our modeling suggested it to be an integral outer membrane protein with eight anti-parallel, membrane-traversing ?-strands. In this report, the outer membrane localization model was confirmed by the structural and functional tolerance of PorT to hexa-histidine (6×His) tag insertions at selected locations within the protein using site-directed mutagenesis. Interestingly, those PorT mutations adversely affecting gingipain secretion enhanced expression of the porT gene but at the same time suppressed the transcription of the gingipain rgpB gene. Further, PorT mutants deficient in gingipain activities produced significantly more di- and tri-aminopeptidase activities. PorT homologues have been found in restricted members of the Bacteroidetes phylum where there is potential for PorT to participate in the maturation and secretion of proteins with characteristic C-terminal domains (CTD). Knowledge of the cellular localisation of PorT will enable analysis of the role of this protein in a new secretory pathway for the export of gingipains and other CTD-class proteins. PMID:19202082

  2. Diagnóstico diferencial en la encefalitis por anticuerpos contra el receptor NMDA

    PubMed Central

    González-Valcárcel, J.; Rosenfeld, M.R.; Dalmau, J.

    2011-01-01

    Resumen Introducción La encefalitis por anticuerpos contra el receptor de NMDA (NMDAR) suele desarrollarse como un síndrome característico de evolución multifásica y diagnóstico diferencial amplio. Pacientes Presentamos a 2 pacientes diagnosticadas de encefalitis por anticuerpos NMDAR con un cuadro clínico típico, pero que inicialmente señaló otras etiologías. Discusión La afectación frecuente de pacientes jóvenes con manifestaciones psiquiátricas prominentes indica frecuentemente otras consideraciones diagnósticas; las más frecuentes son las encefalitis virales, los procesos psiquiátricos y el síndrome neuroléptico maligno. Varios síndromes previamente definidos de manera parcial o descriptiva en adultos y pacientes pediátricos probablemente eran casos de encefalitis anti-NMDAR. Conclusiones La encefalitis anti-NMDAR debe considerarse en pacientes jóvenes con manifestaciones psiquiátricas subagudas, movimientos anormales y alteraciones autonómicas. La caracterización clínica e inmunológica de esta enfermedad ha llevado a la identificación de nuevos anticuerpos que afectan a procesos de memoria, aprendizaje, conducta y psicosis. PMID:20964986

  3. Gestión por competencias: importante para las pymes maquila de pantalón en índigo en la ciudad de Bogotá

    Microsoft Academic Search

    Nathalia Andrea Martínez Valero; Juan Carlos Rincón Gaviria

    2010-01-01

    Este artículo presenta una revisión de literatura internacional y nacional, sobre temas como el de competencias, los tipos de competencias, competencias laborales y proceso de implementación de la gestión por competencias. Adicionalmente, se muestra la reseña sobre la gestión por competencias en Colombia, así como la metodología utilizada para el trabajo de campo con relación al tema del artículo y

  4. Instituto do petrleo e do gs nasce por causa do Brasil e tem

    E-print Network

    Instituto de Sistemas e Robotica

    Instituto do petróleo e do gás nasce por causa do Brasil e tem 110 milhões de euros até 2017&D chegam a 775 milhões de dólares em 2025 A Galp Energia vai aplicar 1% das receitas no Brasil em I&D O. As regras de aproveitamento do financiamento proporcionado através do Brasil impõem, contudo, que as verbas

  5. LA UA EN CIFRAS Evolucin series histricas: Evolucin PDI por Categoras

    E-print Network

    Escolano, Francisco

    LA UA EN CIFRAS Evolución series históricas: Evolución PDI por Categorías Año 2010 Mujer % Hombre-VALI) 5 0,59% 3 0,19% 8 0,33% INVESTIGADOR PDI 6 0,71% 17 1,09% 23 0,95% PROFESOR ASOCIADO 84 9,92% 244 15

  6. Los dioses en sí mismos: lo afrocubano en Requiem por Yarini de Carlos Felipe

    E-print Network

    Linares-Ocanto, Luis

    2000-04-01

    SPRING 2000 43 Los dioses en sí mismos: lo afrocubano en Requiem por Yarini de Carlos Felipe Luis Linares-Ocanto Los elementos de la santería, o la religión afro-cubana, no se integran plenamente al teatro cubano sino hasta el siglo XX.... Anteriormente sólo existían casos de autores aislados que incluyeron algún elemento específico. Hasta entonces, el teatro siempre se dedicaba a criticar y burlarse de las supersticiones del negrito. Ya en este siglo XX es indiscutible que la liturgia negra...

  7. GENE POOL -El juego de cartas del ADN por Mark Goadrich

    E-print Network

    Goadrich, Mark

    GENE POOL - El juego de cartas del ADN por Mark Goadrich http://games.goadrich.com Instrucciones 2 de pares de bases de vuestra mano, para modificar y reordenar una secuencia de ADN común. Sólo investigación actual) y sólo la revelaréis cuando hayais conseguido la secuencia de ADN correcta. A lo largo del

  8. Análise dos Conceitos Astronômicos Apresentados por Professores de Algumas Escolas Estaduais Brasileiras

    NASA Astrophysics Data System (ADS)

    Voelzke, Marcos Rincon; Gonzaga, Edson Pereira

    2011-12-01

    A razão para o desenvolvimento deste trabalho baseia-se no fato de que muitos professores da Educação Básica (EB) não lidam com conceitos relacionados à astronomia, e quando o fazem eles simplesmente seguem livros didáticos que podem conter erros conceituais. Como é de conhecimento geral a astronomia é um dos conteúdos a serem ensinados na EB fazendo parte dos Parâmetros Curriculares Nacionais e das Propostas Curriculares do Estado de São Paulo, mas é um fato, que vários pesquisadores apontam, a existência de muitos problemas no ensino da astronomia. Com o propósito de minimizar algumas dessas deficiências foi realizado um trabalho de pesquisa com a utilização de questionários pré e pós pesquisa, para tanto foi desenvolvido um Curso de Extensão Universitária para professores da Diretoria de Ensino Regional (DE) que abrange Mauá, Ribeirão Pires e Rio Grande da Serra (no Estado de São Paulo) com os seguintes objetivos: levantar concepções alternativas; subsidiar os professores por meio de palestras, debates e workshops, e verificar o sucesso da aprendizagem após o curso, adotando-se como referência, para a análise dos resultados, os dicionários de Língua Portuguesa (FERREIRA, 2004) e Enciclopédico de Astronomia e Astronáutica (MOUR?O, 1995). Portanto, dezesseis questões foram aplicadas antes e após o curso, assim pode-se verificar após a pesquisa que 100,0% dos professores sabiam os nomes das fases da Lua, 97,0% entenderam que o Sistema Solar é composto por oito planetas, 78,1% foram capazes de explicar como ocorre um eclipse lunar, um eclipse solar e um solstício, 72,7% sabiam como explicar a ocorrência das estações do ano; 64,5% explicaram corretamente a ocorrência do equinócio, 89,7% foram capazes de definir adequadamente o termo cometa; 63,6% definiram asteróide, 54,5% meteoro, 58,1% galáxia, e 42,4% planeta. Os resultados obtidos indicam uma aprendizagem significativa por parte dos participantes.

  9. Characterization of plasmid pOR1 from Ornithobacterium rhinotracheale and construction of a shuttle plasmid.

    PubMed

    Jansen, Ruud; Chansiripornchai, Niwat; Gaastra, Wim; van Putten, Jos P M

    2004-10-01

    The bacterium Ornithobacterium rhinotracheale has been recognized as an emerging pathogen in poultry since about 10 years ago. Knowledge of this bacterium and its mechanisms of virulence is still very limited. Here we report the development of a transformation system that enables genetic modification of O. rhinotracheale. The system is based on a cryptic plasmid, pOR1, that was derived from an O. rhinotracheale strain of serotype K. Sequencing indicated that the plasmid consisted of 14,787 nucleotides. Sequence analysis revealed one replication origin and several rep genes that control plasmid replication and copy number, respectively. In addition, pOR1 contains genes with similarity to a heavy-metal-transporting ATPase, a TonB-linked siderophore receptor, and a laccase. Reverse transcription-PCR demonstrated that these genes were transcribed. Other putative open reading frames exhibited similarities with a virulence-associated protein in Actinobacillus actinomycetemcomitans and a number of genes coding for proteins with unknown function. An Escherichia coli-O. rhinotracheale shuttle plasmid (pOREC1) was constructed by cloning the replication origin and rep genes from pOR1 and the cfxA gene from Bacteroides vulgatus, which codes for resistance to the antibiotic cefoxitin, into plasmid pGEM7 by using E. coli as a host. pOREC1 was electroporated into O. rhinotracheale and yielded cefoxitin-resistant transformants. The pOREC1 isolated from these transformants was reintroduced into E. coli, demonstrating that pOREC1 acts as an independent replicon in both E. coli and O. rhinotracheale, fulfilling the criteria for a shuttle plasmid that can be used for transformation, targeted mutagenesis, and the construction of defined attenuated vaccine strains. PMID:15466524

  10. Zinc Finger Nuclease Knock-out of NADPH:Cytochrome P450 Oxidoreductase (POR) in Human Tumor Cell Lines Demonstrates That Hypoxia-activated Prodrugs Differ in POR Dependence*

    PubMed Central

    Su, Jiechuang; Gu, Yongchuan; Pruijn, Frederik B.; Smaill, Jeff B.; Patterson, Adam V.; Guise, Christopher P.; Wilson, William R.

    2013-01-01

    Hypoxia, a ubiquitous feature of tumors, can be exploited by hypoxia-activated prodrugs (HAP) that are substrates for one-electron reduction in the absence of oxygen. NADPH:cytochrome P450 oxidoreductase (POR) is considered one of the major enzymes responsible, based on studies using purified enzyme or forced overexpression in cell lines. To examine the role of POR in HAP activation at endogenous levels of expression, POR knock-outs were generated in HCT116 and SiHa cells by targeted mutation of exon 8 using zinc finger nucleases. Absolute quantitation by proteotypic peptide mass spectrometry of DNA sequence-confirmed multiallelic mutants demonstrated expression of proteins with residual one-electron reductase activity in some clones and identified two (Hko2 from HCT116 and S2ko1 from SiHa) that were functionally null by multiple criteria. Sensitivities of the clones to 11 HAP (six nitroaromatics, three benzotriazine N-oxides, and two quinones) were compared with wild-type and POR-overexpressing cells. All except the quinones were potentiated by POR overexpression. Knocking out POR had a marked effect on antiproliferative activity of the 5-nitroquinoline SN24349 in both genetic backgrounds after anoxic exposure but little or no effect on activity of most other HAP, including the clinical stage 2-nitroimidazole mustard TH-302, dinitrobenzamide mustard PR-104A, and benzotriazine N-oxide SN30000. Clonogenic cell killing and reductive metabolism of PR-104A and SN30000 under anoxia also showed little change in the POR knock-outs. Thus, although POR expression is a potential biomarker of sensitivity to some HAP, identification of other one-electron reductases responsible for HAP activation is needed for their rational clinical development. PMID:24196959

  11. PUBLICACIONES INGRESADAS EN EL CENTRO DE DOCUMENTACIN ESTADSTICA ltimas adquisiciones del Centro de Documentacin Estadstica, por compra y donacin

    E-print Network

    Autonoma de Madrid, Universidad

    Centro de Documentación Estadística, por compra y donación (orden alfabético de títulos) Enero 2014 Atlas/AR.33/11.02.05(46) #12;2 Madrid gran sur metropolitano: territorio, desarrollo regional y medio ambiente

  12. 9 de febrero de 2004 I De conformidad con lo dispuesto por el artculo 30, fraccin XIII del

    E-print Network

    Islas, León

    adquieran vehículos típicos y preferentemente modelos sedán; y que la sustitución se realice sólo cuando lo aumento de sistemas y dispositivos de bloqueo para llamadas a celulares. 5. Los gastos por reuniones de

  13. Comparación de la inducción anestésica rápida con sevofluorano frente a propofol controlada por ordenador a biofase (TCI efecto)

    Microsoft Academic Search

    P. Sepúlveda; G. Núñez; M. Ramos; A. Recart

    2008-01-01

    Resumen OBJETIVO: Comprobar si la inducción inhalatoria sería más rápida que la intravenosa controlada por ordenador (TCI). MATERIAL Y MÉTODOS: Pacientes, ASA 1-2 programa- dos para cirugía electiva, \\

  14. Artculo publicado en "Hidrogeologa y Recursos Hidrulicos", XX: 467-479. 1995 CUANTIFICACION DEL NITROGENO APORTADO POR FERTILIZANTES

    E-print Network

    Castillo, Antonio

    ellos el de Granada capital. INTRODUCCION Encuadre hidrogeológico El acuífero detrítico de la Vega de consumo humano- es el originado por el empleo de fertilizantes, principal fuente de aporte de los nitratos

  15. Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB

    PubMed Central

    Tanabe, Mikio; Iverson, Tina M.

    2009-01-01

    The Neisseria meningitidis outer membrane protein PorB was expressed in Escherichia coli and purified from inclusion bodies by denaturation in urea followed by refolding in buffered LDAO on a size-exclusion column. PorB has been crystallized in three different crystal forms: C222, R32 and P63. The C222 crystal form may contain either one or two PorB monomers in the asymmetric unit, while both the R32 and P63 crystal forms contained one PorB monomer in the asymmetric unit. Of the three, the P63 crystal form had the best diffraction quality, yielding data extending to 2.3?Å resolution. PMID:19851005

  16. Effect of SPM-based cleaning POR on EUV mask performance

    NASA Astrophysics Data System (ADS)

    Choi, Jaehyuck; Lee, Han-shin; Yoon, Jinsang; Shimomura, Takeya; Friz, Alex; Montgomery, Cecilia; Ma, Andy; Goodwin, Frank; Kang, Daehyuk; Chung, Paul; Shin, Inkyun; Cho, H.

    2011-11-01

    EUV masks include many different layers of various materials rarely used in optical masks, and each layer of material has a particular role in enhancing the performance of EUV lithography. Therefore, it is crucial to understand how the mask quality and patterning performance can change during mask fabrication, EUV exposure, maintenance cleaning, shipping, or storage. The fact that a pellicle is not used to protect the mask surface in EUV lithography suggests that EUV masks may have to undergo more cleaning cycles during their lifetime. More frequent cleaning, combined with the adoption of new materials for EUV masks, necessitates that mask manufacturers closely examine the performance change of EUV masks during cleaning process. We have investigated EUV mask quality and patterning performance during 30 cycles of Samsung's EUV mask SPM-based cleaning and 20 cycles of SEMATECH ADT exposure. We have observed that the quality and patterning performance of EUV masks does not significantly change during these processes except mask pattern CD change. To resolve this issue, we have developed an acid-free cleaning POR and substantially improved EUV mask film loss compared to the SPM-based cleaning POR.

  17. Distribucin de los graduados en titulaciones de centros propios en el curso acadmico 2007 / 2008, por centro, plan de estudios y sexo.

    E-print Network

    Autonoma de Madrid, Universidad

    , por centro, plan de estudios y sexo. CENTRO PLAN DE ESTUDIOS TOTAL MUJERES CIENCIAS DIPLOMADO EN académico 2007 / 2008, por centro, plan de estudios y sexo. CENTRO PLAN DE ESTUDIOS TOTAL MUJERES ESC. UNIV

  18. Phase Variation of PorA, a Major Outer Membrane Protein, Mediates Escape of Bactericidal Antibodies by Neisseria meningitidis

    PubMed Central

    Tauseef, Isfahan; Ali, Youssif M.

    2013-01-01

    Several outer membrane proteins of Neisseria meningitidis are subject to phase variation due to alterations in simple sequence repeat tracts. The PorA protein is a major outer membrane protein and a target for protective host immune responses. Phase variation of PorA is mediated by a poly-G repeat tract present within the promoter, leading to alterations in protein expression levels. N. meningitidis strain 8047 was subjected to serial passage in the presence of P1.2, a PorA-specific bactericidal monoclonal antibody. Rapid development of resistance to bactericidal activity was associated with a switch in the PorA repeat tract from 11G to 10G. Phase variants with a 10G repeat tract exhibited a 2-fold reduction in surface expression of PorA protein. A mutS mutant of strain 8047, with an elevated phase variation rate, exhibited a higher rate of escape and an association of escape with 10G and 9G variants, the latter having a 13-fold reduction in surface expression of PorA. We conclude that graduated reductions in the surface expression of outer membrane proteins mediated by phase variation enable meningococci to escape killing in vitro by bactericidal antibodies. These findings indicate how phase variation could have a major impact on immune escape and host persistence of meningococci. PMID:23403557

  19. Effects of heme precursors on CYP1A2 and POR expression in the baculovirus/Spodoptera frugiperda system?

    PubMed Central

    Lu, Huiyuan; Ma, Jun; Liu, Nian; Wang, Shoulin

    2010-01-01

    Objective CYP1A2 and NADPH-CYP450 oxidoreductase (POR) were expressed in the baculovirus/Spodoptera frugiperda (sf9) system. The aim of this study was to investigate the effects of heme precursors on the expression of CYP1A2 and POR. Methods The heme precursors [?-Aminolaevulinic Acid (5-ALA), Fe3+ and hemin] were introduced into the system to evaluate their effects on the expression of CYP1A2, POR and their co-expression. All the proteins were identified using immunoblotting, CO-difference spectroscopy, or cytochrome c assay. Results In the present study, functional CYP1A2 and POR were successfully expressed in the baculovirus/sf9 system, and both of them showed high activities. Co-addition of 5-ALA and Fe3+ significantly improved expression of CYP1A2 by about 50% compared with the addition of 5-ALA, Fe3+ or hemin alone. Either co-addition of 5-ALA and Fe3+ or addition of 5-ALA or Fe3+ alone improved the POR expression level 2 fold and its activity 7-10 fold compared with control (no addition). However, unlike CYP1A2, there was no difference between the co-addition and addition of these heme precursors alone. Different ratios of BvCYP1A2 to BvPOR also affected the co-expression of CYP1A2 and POR, with a 3:1 ratio of BvCYP1A2 / BvPOR significantly increasing their co-expression. Surprisingly, the addition of 0.1 mM 5-ALA or Fe3+ alone, but not their co-addition, could significantly improve the CYP1A2 and POR co-expression (P < 0.05). Conclusion 5-ALA and Fe3+ increased the expression of CYP1A2 and POR in a baculovirus/sf9 system, but the pattern of their expression was different between their expression alone and co-expression. PMID:23554636

  20. Using the PORS Problems to Examine Evolutionary Optimization of Multiscale Systems

    SciTech Connect

    Reinhart, Zachary [Ames Laboratory; Molian, Vaelan [Ames Laboratory; Bryden, Kenneth [Ames Laboratory

    2013-01-01

    Nearly all systems of practical interest are composed of parts assembled across multiple scales. For example, an agrodynamic system is composed of flora and fauna on one scale; soil types, slope, and water runoff on another scale; and management practice and yield on another scale. Or consider an advanced coal-fired power plant: combustion and pollutant formation occurs on one scale, the plant components on another scale, and the overall performance of the power system is measured on another. In spite of this, there are few practical tools for the optimization of multiscale systems. This paper examines multiscale optimization of systems composed of discrete elements using the plus-one-recall-store (PORS) problem as a test case or study problem for multiscale systems. From this study, it is found that by recognizing the constraints and patterns present in discrete multiscale systems, the solution time can be significantly reduced and much more complex problems can be optimized.

  1. Effect of sequence variation in meningococcal PorA outer membrane protein on the effectiveness of a hexavalent PorA outer membrane vesicle vaccine in toddlers and school children.

    PubMed

    Findlow, Jamie; Lowe, Ann; Deane, Sarah; Balmer, Paul; van den Dobbelsteen, Germie; Dawson, Maureen; Andrews, Nick; Borrow, Ray

    2005-04-01

    Though meningococcal conjugate vaccines are effective against serogroup C, there is currently no vaccine solution for serogroup B disease. PorA outer membrane protein (OMP) is a potential serogroup B vaccine candidate. A hexavalent PorA outer membrane vesicle (OMV) vaccine has been evaluated in phase I and II trials with promising results. However, considerable sequence variation occurs in the variable regions (VRs) encoding these serosubtypes. By using five wild type P1.19,15 variant strains we examined the serum bactericidal antibody (SBA) titres from sera collected from toddlers and school children pre- and post-vaccination. The numbers of subjects with SBA titres of <4, 4 and > or = 8 varied greatly between the different strains. This was also reflected when > or = 4-fold rises in SBA titres were examined. This finding in sera from toddlers and school children may have implications for PorA based vaccines. PMID:15780445

  2. Obtención de la curva de luz en la ocultación de 35 Sgr por Júpiter el 6 de marzo de 1996

    NASA Astrophysics Data System (ADS)

    Paolantonio, S.; Duffard, R.; Carranza, G.

    La ocultación de la estrella de quinta magnitud 35 Sgr por Júpiter, se produjo el 6 de Marzo de 1996 a las 13 hs. TU. El objetivo era medir el cambio del flujo de la estrella en el ingreso y egreso por el limbo del planeta. Con estos datos se pueden determinar parámetros físicos del planeta (radio, eccentricidad) y de su atmósfera (escala de altura, temperatura, densidad, presión) Para lograr ésto se programó la cámara CCD TH 7896 1024 x 1025 instalada en el telescopio de 1.54 m de Bosque Alegre con el objetivo de lograr 2 imágenes por segundo. De esta forma se obtuvieron 2100 imágenes de la inmersión y otras tantas de la emersión. Hubo que tener grandes precauciones para evitar la saturación del CCD ya que la observación se realizó de día. En este momento las imágenes se encuentran en el Department of Planetary Sciences, Lunar and Planetary Laboratory, University of Arizona, para su reducción.

  3. Memorias Tercer Congreso Internacional sobre la Enseñanza de la Matemática Asistida por Computadora ANÁLISIS DE LOS REGISTROS DE REPRESENTACIÓN SEMIÓTICA PUESTOS EN JUEGO POR ALUMNOS EN LA RESOLUCIÓN NUMÉRICA DE ECUACIONES POLINÓMICAS

    Microsoft Academic Search

    Estela E. Rechimont

    Resumen Las representaciones juegan un rol fundamental en l os procesos de construcción de conceptos, por lo qu e son importantes en la enseñanza, aprendizaje y comunicación del conocimiento matemático (Hitt, 1996). El presente trabajo muestra los resultados de un es tudio en donde analizamos el tipo de representacion es que utilizan los alumnos en la resolución de problemas relativos

  4. Antitumor actinopyranones produced by Streptomyces albus POR-04-15-053 isolated from a marine sediment.

    PubMed

    Schleissner, Carmen; Pérez, Marta; Losada, Alejandro; Rodríguez, Pilar; Crespo, Cristina; Zúñiga, Paz; Fernández, Rogelio; Reyes, Fernando; de la Calle, Fernando

    2011-07-22

    Four new antitumor pyranones, PM050511 (1), PM050463 (2), PM060054 (3), and PM060431 (4), were isolated from the cell extract of the marine-derived Streptomyces albus POR-04-15-053. Their structures were elucidated by a combination of spectroscopic methods, mainly 1D and 2D NMR and HRESIMS. They consist of an ?-methoxy-?-pyrone ring containing a highly substituted tetraene side chain glycosylated at C-10 in the case of 1 and 4. Compounds 1 and 4 displayed strong cytotoxicity against three human tumor cell lines with GI?? values in the submicromolar range, whereas 2 showed subnanomolar activity as an inhibitor of EGFR-MAPK-AP1-mediated mitogenic signaling, causing inhibition of EGF-mediated AP1 trans-activation and EGF-mediated ERK activation and slight inhibition of EGF-mediated JNK activation. Taken together, these results suggest that members of the pyranone family of compounds could be developed as potential antitumor agents. PMID:21718029

  5. Estimaciones de Prevalencia del VIH por Género y Grupo de Riesgo en Tijuana, México: 2006

    PubMed Central

    Iñiguez-Stevens, Esmeralda; Brouwer, Kimberly C.; Hogg, Robert S.; Patterson, Thomas L.; Lozada, Remedios; Magis-Rodriguez, Carlos; Elder, John P.; Viani, Rolando M.; Strathdee, Steffanie A.

    2010-01-01

    OBJETIVO Estimar la prevalencia del VIH en adultos de 15-49 años de edad en Tijuana, México - en la población general y en subgrupos de riesgo en el 2006. METODOS Se obtuvieron datos demográficos del censo Mexicano del 2005, y la prevalencia del VIH se obtuvo de la literatura. Se construyó un modelo de prevalencia del VIH para la población general y de acuerdo al género. El análisis de sensibilidad consistió en estimar errores estándar del promedio-ponderado de la prevalencia del VIH y tomar derivados parciales con respecto a cada parámetro. RESULTADOS La prevalencia del VIH es 0.54%(N = 4,347) (Rango: 0.22%–0.86%, (N = 1,750–6,944)). Esto sugiere que 0.85%(Rango: 0.39%–1.31%) de los hombres y 0.22%(Rango: 0.04%–0.40%) de las mujeres podrían ser VIH-positivos. Los hombres que tienen sexo con hombres (HSH), las trabajadoras sexuales usuarias de drogas inyectables (MTS-UDI), MTS-noUDI, mujeres UDI, y los hombres UDI contribuyeron las proporciones más elevadas de personas infectadas por el VIH. CONCLUSIONES El número de adultos VIH-positivos entre subgrupos de riesgo en la población de Tijuana es considerable, marcando la necesidad de enforcar las intervenciones de prevención en sus necesidades específicas. El presente modelo estima que hasta 1 en cada 116 adultos podrían ser VIH-positivos. PMID:19685824

  6. Functional POR A503V is associated with the risk of bladder cancer in a Chinese population

    PubMed Central

    Xiao, Xue; Ma, Gaoxiang; Li, Shushu; Wang, Meilin; Liu, Nian; Ma, Lan; Zhang, Zhan; Chu, Haiyan; Zhang, Zhengdong; Wang, Shou-Lin

    2015-01-01

    Human cytochrome P450 oxidoreductase (POR) plays important roles in the metabolism of exogenous carcinogens and endogenous sterol hormones. However, few studies have explored the association between POR variants and the risk of bladder cancer. In this study, we first sequenced all 16 POR exons among 50 randomly selected controls, and found three variants, rs1135612, rs1057868 (A503V) and rs2228104, which were then assessed the relation to risk of bladder cancer in a case-control study of 1,050 bladder cancer cases and 1,404 cancer-free controls in a Chinese population. People with A503V TT genotype have a decreased risk of bladder cancer in a recessive model (TT vs. CC/CT, OR?=?0.73, 95% CI?=?0.57–0.93), which was more pronounced among elderly male, non-smoking, subjects. Especially, A503V TT genotype showed a protective effect in the invasive tumor stage. Functional analysis revealed that A503V activity decreased in cytochrome c reduction (50.5 units/mg vs. 135.4 units/mg), mitomycin C clearance (38.3% vs. 96.8%), and mitomycin C-induced colony formation (78.0 vs 34.3 colonies per dish). The results suggested that POR A503V might decrease the risk of bladder cancer by reducing its metabolic activity, and should be a potential biomarker for predicting the susceptibility to human bladder cancer. PMID:26123203

  7. TABLA DE ADAPTACIONES DE ASIGNATURAS SUPERADAS EN EL PROGRAMA SIMULTNEO DERECHO-ADE POR LAS ASIGNATURAS DEL GRADO DE DERECHO

    E-print Network

    Escolano, Francisco

    TABLA DE ADAPTACIONES DE ASIGNATURAS SUPERADAS EN EL PROGRAMA SIMULTÁNEO DERECHO-ADE POR LAS ASIGNATURAS DEL GRADO DE DERECHO 1º CURSO DERECHO Créditos Tipo DERECHO-ADE Créditos Tipo Derecho Romano 6 FB Derecho Romano 6 FB Historia del Derecho Español y de las Instituciones 6 FB Historia del Derecho Español

  8. TABLA DE ADAPTACIONES DE ASIGNATURAS SUPERADAS EN EL PROGRAMA SIMULTNEO DERECHO-ADE POR LAS ASIGNATURAS DEL GRADO DE ADE

    E-print Network

    Escolano, Francisco

    TABLA DE ADAPTACIONES DE ASIGNATURAS SUPERADAS EN EL PROGRAMA SIMULTÁNEO DERECHO-ADE POR LAS ASIGNATURAS DEL GRADO DE ADE 1º CURSO ADE Créditos Tipo DERECHO-ADE Créditos Tipo Matemáticas I 6 FB Economía de la Empresa 6 FB Derecho de la Empresa 6 FB Derecho Mercantil I 6 OB Matemáticas II 6 FB

  9. Derechos Bsicos de Ausencia La Ley de Ausencia Familiar y Mdica (FMLA-por sus siglas en ingls) exige

    E-print Network

    Rock, Chris

    Derechos Básicos de Ausencia La Ley de Ausencia Familiar y Médica (FMLA-por sus siglas en inglés condición de salud seria que le impida al empleado desempeñar su puesto. Derechos de Ausencia Para Familias avisado de una llamada a estado de servicio activo bajo cobertura pueden usar su derecho de ausencia de 12

  10. Molecular Epidemiology of Neisseria gonorrhoeae: Sequence Analysis of the porB Gene Confirms Presence of Two Circulating Strains

    Microsoft Academic Search

    Magnus Unemo; Per Olcen; Torsten Berglund; Jan Albert; Hans Fredlund

    2002-01-01

    The phenotypic and genotypic characteristics of Neisseria gonorrhoeae strains fluctuate over time both locally and globally, and highly discriminative and precise characterization of the strains is essential. Conventional characterization of N. gonorrhoeae strains for epidemiological purposes is mostly based on phenotypic methods, which have some inherent limitations. In the present study sequence analysis of porB1b gene sequences was used for

  11. [Professor Frantisek Por MD and Professor Robert Klopstock MD, students at Budapest and Prague Faculties of Medicine].

    PubMed

    Mydlík, M; Derzsiová, K

    2010-11-01

    Professor Frantisek Por MD and Professor Robert Klopstock MD were contemporaries, both born in 1899, one in Zvolen, the other in Dombovar, at the time of Austro-Hungarian Monarchy. Prof. Por attended the Faculty of Medicine in Budapest from 1918 to 1920, and Prof. Klopstock studied at the same place between 1917 and 1919. From 1920 until graduation on 6th February 1926, Prof. Por continued his studies at the German Faculty of Medicine, Charles University in Prague. Prof. Klopstock had to interrupt his studies in Budapest due to pulmonary tuberculosis; he received treatment at Tatranske Matliare where he befriended Franz Kafka. Later, upon Kafka's encouragement, he changed institutions and continued his studies at the German Faculty of Medicine, Charles University in Prague, where he graduated the first great go. It is very likely that, during their studies in Budapest and Prague, both professors met repeatedly, even though their life paths later separated. Following his graduation, Prof. Por practiced as an internist in Prague, later in Slovakia, and from 1945 in Kosice. In 1961, he was awarded the title of university professor of internal medicine at the Faculty of Medicine, Pavol Jozef Safarik University in Kosice, where he practiced until his death in 1980. Prof. Klopstock continued his studies in Kiel and Berlin. After his graduation in 1933, he practiced in Berlin as a surgeon and in 1938 left for USA. In 1962, he was awarded the title of university professor of pulmonary surgery in NewYork, where he died in 1972. PMID:21250499

  12. Caracterización fisicoquímica, funcional y química de harinas nixtamalizadas de maíz obtenidas por calentamiento óhmico y proceso tradicional

    Microsoft Academic Search

    M. Gaytán-Martínez; J. D. C. Figueroa; P. A. Vázquez-Landaverde; E. Morales-Sánchez; H. E. Martínez-Flores; M. L. Reyes-Vega

    2012-01-01

    Se caracterizaron harinas de maíz nixtamalizadas (HMN) obtenidas por calentamiento óhmico (HCO), proceso tradicional (HPT) y harina comercial (HC) en su composición química, capacidad e índice de absorción de agua (CAA, IAA), índice de solubilidad (ISA), viscosidad, rendimiento, humedad y textura de las masas y tortillas. Las HCO presentaron diferentes grados de gelatinización de acuerdo a los resultados de IAA,

  13. Listado de cursos de verano por fecha de realizacin SEMANA DEL 1 DE JULIO AL 7 DE JULIO

    E-print Network

    Escolano, Francisco

    Listado de cursos de verano por fecha de realización SEMANA DEL 1 DE JULIO AL 7 DE JULIO El puerto Curso Rromipen/gitanidad: historia y cultura del pueblo gitano (1er.) Economía del bien común: economía Taller de dirección orquestal -activo. CURSO CANCELADO Taller de dirección orquestal -oyente. CURSO

  14. Modelo de Gestin de Espacios de la Universidad de Alicante Criterio A: Superficie por tipo de contratacin

    E-print Network

    Escolano, Francisco

    Modelo de Gestión de Espacios de la Universidad de Alicante Criterio A: Superficie por tipo de criterio de reparto de espacio entre el personal que trabaja en la UA que sea capaz de considerar la existencia de espacios compartidos entre distintos trabajadores con tipos de contratación igualmente diversos

  15. FLORE BACTRIENNE AROBIE DES CAVITS NASALES DU LAPIN D'LEVAGE

    E-print Network

    Paris-Sud XI, Université de

    , Alcaligenes faecalis and Escherichia coli. However, young weaned rabbits were more often Pasteurella carriers, Streptococcus faecalis, Pasteurella multocida, Staphylococcus aureus, Bacillus sp., Branhamella catarrhalis

  16. ¿Obtienen mejores resultados los estudiantes que siguen cursos por Internet que los que siguen cursos presenciales? Reflexiones y breve revisión de algunos resultados empíricos

    Microsoft Academic Search

    Johan Lundberg; David Castillo Merino; Mounir Dahmani

    2008-01-01

    El aumento del número de cursos en línea impartidos por las universidades ha sido espectacular en los dos últimos años. Hoy en día, muchas universidades ofrecen incluso programas de titulación completa por Internet. Los estudiantes cuentan con acceso 24 horas a las instrucciones y los contenidos de las clases, p. ej., en formato de vídeo. En cierto modo, el uso

  17. La responsabilidad Social Empresarial (RSE) en Europa: la apuesta por un nuevo modelo de empresa. Una revisión crítica desde la ética empresarial

    Microsoft Academic Search

    Elsa González Esteban; Vicente Domingo García Marzá

    2006-01-01

    El presente artículo tiene un doble objetivo. Por una parte, poner de manifiesto el desarrollo del discurso europeo respecto a la necesidad de asunción de valores y principios sociales y medioambientales en la gestión y dirección de las empresas con la finalidad de alcanzar altas cotas de competitividad y de cohesión social. Por otra parte, ofrecer un modelo de Responsabilidad

  18. Exhibicin de Arte sobre el Ocano Coastal America Por favor complete, imprima y firme el Formato de Registro y el Formato de Liberacin mostrados a

    E-print Network

    Tullos, Desiree

    el participante es menor a 18 años de edad, este Formato deberá ser firmado tanto por el participante participante es menor de 18 años de edad, la Liberación deberá ser firmada por alguno de los padres o tutor del Registro Información del Participante Nombre: Edad: Grado (si aplica): Dirección postal: Ciudad: Estado

  19. Características psicométricas de las versiones americana y española de la escala TDA-H (Trastorno por Déficit de Atención con Hiperactividad): un estudio comparativo

    Microsoft Academic Search

    Nekane Balluerka; Juana Gómez; William A. Stock; Linda C. Caterino

    2000-01-01

    El presente artículo se centra en el estudio psicométrico de una es- cala para el diagnóstico del Tr a s t o rno por Déficit de Atención con H i p e ra c t ividad en adultos. Dicho tra s t o rno fue descubierto a pri n c i- pios de siglo en la población infantil por un

  20. RESOLUCIN DEL RECTOR DE LA UNIVERSIDAD POLITCNICA DE MADRID, DE 18 DE JULIO DE 2014, POR LA QUE SE AMPLIA EL PLAZO

    E-print Network

    Cardeñosa, Jesús

    RESOLUCIÓN DEL RECTOR DE LA UNIVERSIDAD POLITÉCNICA DE MADRID, DE 18 DE JULIO DE 2014, POR LA QUE RECTORAL DE 19 DE MAYO DE 2014. La Universidad Politécnica de Madrid tiene convocado, por Resolución de lo Contencioso Administrativo de Madrid. Madrid, 18 de julio de 2014 EL RECTOR, Carlos Conde

  1. CYP2C9*2 allele increases risk for hypoglycemia in POR*1/*1 type 2 diabetic patients treated with sulfonylureas.

    PubMed

    Ragia, G; Tavridou, A; Elens, L; Van Schaik, R H N; Manolopoulos, V G

    2014-01-01

    It is previously shown that carriers of the defective allele CYP2C9*3 that leads to impaired sulfonylurea metabolism are at increased sulfonylurea-induced hypoglycemia risk due to diminished drug metabolism, whereas no effect of CYP2C9*2 allele was found. Recently, a polymorphism in P450 oxidoreductase (POR) gene, assigned as POR*28 allele, was associated with increased CYP2C9 activity. The aim of this study was to assess i) the effect of POR*28 allele on sulfonylurea-induced hypoglycemia risk and ii) the association of CYP2C9*2 allele with hypoglycemia risk in non-carriers of POR*28 allele. The study group consisted of 176 patients with diagnosed type 2 diabetes mellitus (T2DM) treated with sulfonylureas, of whom 92 patients had experienced at least one drug-associated hypoglycemic event (cases), while 84 had never experienced a hypoglycemic event (controls). POR*28 allele was detected by use of real-time TaqMan PCR. POR*28 allele was not associated with sulfonyl-urea-induced hypoglycemia. In POR*1/*1 patients, CYP2C9*1/*2 genotype was more common in cases than in controls (32.7 vs. 14.3%, p=0.041). In a model adjusted for age, BMI, duration of T2DM and renal function, and POR*1/*1 entered as a selection variable, CYP2C9*2 allele increased the hypoglycemia risk in response to sulfonylurea (odds ratio: 3.218, p=0.031). In conclusion, our results suggest that POR*28 allele is masking the association of CYP2C9*2 allele with sulfonyl-urea-induced hypoglycemia. Therefore, POR*28 allele is an important source of CYP2C9 activity variability and combined with CYP2C9 gene poly-morphisms may explain individual variability in the effect of sulfonylureas. PMID:24464600

  2. Pincharse sin infectarse: estrategias para prevenir la infección por el VIH y el VHC entre usuarios de drogas inyectables

    PubMed Central

    MATEU-GELABERT, P.; FRIEDMAN, S.; SANDOVAL, M.

    2011-01-01

    Resumen Objetivo Desde principios de los noventa, en la ciudad de Nueva York se han implementado con éxito programas para reducir la incidencia del virus de la inmunodeficiencia humana (VIH) y, en menor medida, del virus de la hepatitis C (VHC). A pesar de ello, aproximadamente el 70% de los usuario de drogas inyectables (UDI) están infectados por el VHC. Queremos investigar cómo el 30% restante se las ha arreglado para no infectarse. El Staying safe (nombre original del estudio) explora los comportamientos y mecanismos que ayudan a evitar la infección por el VHC y el VIH a largo plazo. Material y métodos Hemos utilizado el concepto de «desviación positiva» aplicado en otros campos de salud pública. Estudiamos las estrategias, prácticas y tácticas de prevención de aquellos UDI que, viviendo en contextos de alta prevalencia, se mantienen sin infectar por VIH y el VHC, a pesar de haberse inyectado heroína durante años. Los resultados preliminares presentados en este artículo incluyen el análisis de las entrevistas realizadas a 25 UDI (17 doble negativos, 3 doble positivos y 5 con infección por el VHC y sin infección por el VIH). Se usaron entrevistas semiestructuradas que exploraban con detalle la historia de vida de los sujetos, incluyendo su consumo de drogas, redes sociales, contacto con instituciones, relaciones sexuales y estrategias de protección y vigilancia. Resultados La intencionalidad es importante para no infectarse, especialmente durante períodos de involución (períodos donde hay un deterioro económico y/o social que llevan al que se inyecta a situaciones de mayor riesgo). Presentamos tres dimensiones independientes de intencionalidad que conllevan comportamientos que pueden ayudar a prevenir la infección: a) evitar «el mono» (síntomas de abstención) asegurando el acceso a la droga; b) «llevarlo bien» para no convertirse en un junkie y así evitar la «muerte social» y la falta de acceso a los recursos, y c) seguir sin infectarse por el VIH (sólo un doble negativo tuvo un amplio conocimiento sobre la hepatitis C). Estas intencionalidades no son mutuamente excluyentes. La presencia de varias refuerza la puesta en práctica a diario de comportamientos que pueden ayudar al que se inyecta a mantenerse libre de infecciones durante años. Algunas prácticas que hemos identificado se implementan en grupo y se comunican de UDI a UDI, de esta manera se extienden entre algunas redes sociales de UDI. Conclusiones Los UDI que permanecen sin infectarse planean e implementan estrategias de prevención en circunstancias donde otros UDI aplican prácticas de riesgo. El mantenimiento de la no infección no es, por lo tanto, un resultado del azar, sino más bien el resultado del esfuerzo (agencia) de los UDI. Investigar y extender estas estrategias y tácticas a través de programas de prevención podría contribuir a la prevención del VIH y el VHC. PMID:21915175

  3. [Susceptibilities of bacteria isolated from patients with lower respiratory infectious diseases to antibacterial agents (2008)].

    PubMed

    Goto, Hajime; Iwasaki, Mitsuhiro

    2015-02-01

    From October 2008 to September 2009, we collected the specimen from 374 patients with lower respiratory tract infections in 15 institutions in Japan, and investigated the susceptibilities of isolated bacteria to various antibacterial agents and patients' characteristics. Of 423 strains that were isolated from specimen (mainly from sputum) and assumed to be bacteria causing in infection, 421 strains were examined. The isolated bacteria were: Staphylococcus aureus 78, Streptococcus pneumoniae 78, Haemophilus influenzae 89, Pseudomonas aeruginosa (non-mucoid) 61, P. aeruginosa (mucoid) 19, Klebsiella pneumoniae 28, and Moraxella catarrhalis 32. Of 78 S. aureus strains, those with 2 ?g/mL or less of MIC of oxacillin (methicillin-susceptible S. aureus: MSSA) and those with 4 ?g/mL or more of MIC of oxacillin (methicillin-resistant S. aureus: MRSA) were 34 (43.6%) and 44 (56.4%) strains, respectively. Against MSSA, imipenem had the most potent antibacterial activity and inhibited the growth of all strains at 0.063 ?g/mL or less. Against MRSA vancomycin and arbekacin showed the potent activity and inhibited the growth of all the strains at 1 and 2 ?g/mL, respectively. Linezolid also showed the great activity and inhibited the growth of all the strains at 1 ?g/mL. Carbapenems and penems showed the most potent activities against S. pneumoniae and panipenem inhibited the growth of all the strains at 0.125 ?g/mL. Imipenem and faropenem also had a preferable activity and inhibited the growth of all the strains at 0.25 and 1 ?g/mL, respectively. In contrast, there were high-resistant strains (MIC: > 128 ?g/mL) for erythromycin (43.6%) and clindamycin (19.2%). Against H. influenzae, levofloxacin showed the most potent activity and its MIC90 was 0.063 ?g/mL or less. Tobramycin showed the most potent activity against P. aeruginosa (mucoid) and its MIC90 was 2 ?g/mL. Against the non-mucoid type of P. aeruginosa, tobramycin and ciprofloxacin had the most potent activity and its MIC90 was 2 ?g/mL. Against K. pneumoniae, cefozopran had the most potent activity and inhibited the growth of all the strains at 0.063 ?g/mL or less. All the antibacterial agents except ampicillin generally showed a potent activity against M. catarrhalis and the MIC90 of them were 2 ?g/mL or less. The majority number (57.7%) of the patients with respiratory infection were aged 70 years or older. Bacterial pneumonia and chronic bronchitis accounted for 40.9% and 32.9% of all the respiratory infection, respectively. The bacteria frequently isolated from the patients with bacterial pneumonia were S. aureus (20.5%), S. pneumoniae (21.1%), and H. influenzae (22.8%). S. aureus (21.7%) and P. aeruginosa (24.6%) also were frequently isolated from the patients with chronic bronchitis. The bacteria frequently isolated from the patients were S. pneumoniae (23.4%) and H. influenzae (25.1%) before administration of the antibacterial agents. The bacteria frequently isolated from the patients previously treated with cephems and macrolides were P. aeruginosa, and the isolation frequencies were 41.4% and 40.0%, respectively. PMID:25980051

  4. [Susceptibilities of bacteria isolated from patients with lower respiratory infectious diseases to antibacterial agents (2007)].

    PubMed

    Goto, Hajime; Iwasaki, Mitsuhiro

    2015-02-01

    From October 2007 to September 2008, we collected the specimen from 362 patients with lower respiratory tract infections in 14 institutions in Japan, and investigated the susceptibilities of isolated bacteria to various antibacterial agents and patients' characteristics. Of 413 strains that were isolated from specimen (mainly from sputum) and assumed to be bacteria causing in infection, 412 strains were examined. The isolated bacteria were: Staphylococcus aureus 65, Streptococcus pneumoniae 90, Haemophilus influenzae 88, Pseudomonas aeruginosa (non-mucoid) 53, P. aeruginosa (mucoid) 13, Klebsiella pneumoniae 19, and Moraxella catarrhalis 41. Of 65 S. aureus strains, those with 2 ?g/mL or less of MIC of oxacillin (methicillin-susceptible S. aureus: MSSA) and those with 4 ?g/mL or more of MIC of oxacillin (methicillin-resistant S. aureus: MRSA) were 38 (58.5%) and 27 (41.5%) strains, respectively. Against MSSA, imipenem had the most potent antibacterial activity and inhibited the growth of all strains at 0.063 ?g/mL or less. Against MRSA, vancomycin and arbekacin showed the most potent activity and inhibited the growth of all the strains at 2 ?g/mL. Linezolid also showed the same activity as them. Carbapenems and penems showed the most potent activities against S. pneumoniae and in particular, panipenem inhibited the growth of all the strains at 0.063 ?g/mL or less. Imipenem and faropenem also had a preferable activity and inhibited the growth of all the strains at 0.25 and 1 ?g/mL, respectively. In contrast, there were high-resistant strains (MIC: over 128 ?g/mL) for erythromycin (38.2%) and clindamycin (18.0%). Against H. influenzae, levofloxacin showed the most potent activity and its MIC90 was 0.063 ?g/mL or less. Meropenem showed the most potent activity against P. aeruginosa (mucoid) and its MIC90 was 0.5 ?g/mL. Against P. aeruginosa (non-mucoid), tobramycin had the most potent activity and its MIC90 was 2 ?g/mL. Against K. pneumoniae, cefozopran had the most potent activity and inhibited the growth of all the strains at 0.063 ?g/mL or less. Also, all the antibacterial agents except ampicillin generally showed a potent activity against M. catarrhalis and the MIC90 of them were 2 ?g/mL or less. The approximately half the number (45.9%) of the patients with respiratory infection were aged 70 years or older. Bacterial pneumonia and chronic bronchitis accounted for 44.8% and 31.5% of all the respiratory infection, respectively. The bacteria frequently isolated from the patients with bacterial pneumonia were S. aureus (21.9%), S. pneumoniae (20.8%), and H. influenzae (18.6%). S. pneumoniae (27.1%), H. influenzae (24.0%) and P. aeruginosa (17.8%) also were frequently isolated from the patients with chronic bronchitis. Before the drug administration, the bacteria frequently isolated from the patients were S. pneumoniae (23.9%) and H. influenzae (23.6%). The bacteria frequently isolated from the patients treated with macrolides were S. pneumoniae, and their isolation frequencies were 34.8%. PMID:25980050

  5. [Susceptibilities of bacteria isolated from patients with lower respiratory infectious diseases to antibacterial agents (2009)].

    PubMed

    Goto, Hajime; Kumagai, Shigeru

    2015-02-01

    From October 2009 to September 2010, we collected the specimen from 432 patients with lower respiratory tract infections in 16 institutions in Japan, and investigated the susceptibilities of isolated bacteria to various antibacterial agents and patients' characteristics. All of 479 strains that were isolated from specimen (mainly from sputum) and assumed to be bacteria causing in infection, were examined. The isolated bacteria were: Staphylococcus aureus 90, Streptococcus pneumoniae 74, Haemophilus influenzae 82, Pseudomonas aeruginosa (non-mucoid) 60, P. aeruginosa (mucoid) 31, Klebsiella pneumoniae 41, and Moraxella catarrhalis 34. Of 90 S. aureus strains, those with 2 ?g/mL or less of MIC of oxacillin (methicillin-susceptible S. aureus: MSSA) and those with 4 ?g/mL or more of MIC of oxacillin (methicillin-resistant S. aureus: MRSA) were 43 (47.8%) and 47 (52.2%) strains, respectively. Against MSSA, imipenem had the most potent antibacterial activity and inhibited the growth of all strains at 0.063 ?g/mL or less. Against MRSA, vancomycin and arbekacin showed the potent activity and inhibited the growth of all the strains at 2 and 4 ?g/mL, respectively. Linezolid also showed the great activity and inhibited the growth of all the strains at 2 ?g/mL. Carbapenems and penems showed the most potent activities against S. pneumoniae and panipenem inhibited the growth of all the strains at 0.125 ?g/mL. Imipenem and faropenem also had a preferable activity and inhibited the growth of all the strains at 0.25 and 0.5 ?g/mL, respectively. In contrast, there were high-resistant strains (MIC: > 128 ?g/mL) for erythromycin (51.4%) and clindamycin (35.1%). Against H. influenzae, levofloxacin showed the most potent activity and its MIC90 was 0.063 ?g/mL or less. Meropenem showed the most potent activity against P. aeruginosa (mucoid) and its MIC90 was 1 ?g/mL. Against the non-mucoid type of P. aeruginosa, tobramycin had the most potent activity and its MIC90 was 2 ?g/mL. Against K. pneumoniae, cefozopran had the most potent activity and inhibited the growth of all the strains at 0.125 ?g/mL or less. All the antibacterial agents except ampicillin generally showed a potent activity against M. catarrhalis and the MIC90 of them were 2 ?g/mL or less. The majority number (60.0%) of the patients with respiratory infection was aged 70 years or older. Bacterial pneumonia and chronic bronchitis accounted for 48.8% and 31.7% of all the respiratory infection, respectively. The bacteria frequently isolated from the patients with bacterial pneumonia were S. aureus (21.5%), S. pneumoniae (20.2%), and H. influenzae (16.7%). S. aureus (21.9%) and P. aeruginosa (20.0%) also were frequently isolated from the patients with chronic bronchitis. The bacteria frequently isolated from the patients were S. pneumoniae (21.5%) and H. influenzae (20.5%) before administration of the antibacterial agents. The bacteria frequently isolated from the patients previously treated with cephems and macrolides were P. aeruginosa, and the isolation frequencies were 28.6% and 47.2%, respectively. PMID:25980052

  6. Spectrum of activity, mutation rates, synergistic interactions, and the effects of pH and serum proteins for fusidic acid (CEM-102).

    PubMed

    Biedenbach, Douglas J; Rhomberg, Paul R; Mendes, Rodrigo E; Jones, Ronald N

    2010-03-01

    Fusidic acid (CEM-102) is a steroidal antimicrobial agent with focused Gram-positive activity that acts by preventing bacterial protein synthesis via interacting with elongation factor G. A collection of 114 wild-type isolates (> 80 species) was used to define the contemporary limits of fusidic acid spectrum against Gram-positive and Gram-negative species. Reference broth microdilution and anaerobic agar dilution methods were performed. Modifications of standardized test methods included adding 10% human serum and adjusting the medium pH to 5, 6, and 8. Synergy was assessed by the checkerboard method and time-kill studies. Mutational rates to resistance were determined at 4 x, 8 x, and 16 x MIC. Against Gram-positive pathogens, fusidic acid MIC values ranged from 0.06 to 32 microg/mL with the greatest potency against Staphylococcus aureus, Corynebacterium spp., and Micrococcus luteus (MIC results, 0.25, < or = 0.12, and < or = 0.5 microg/mL, respectively). Enterococci and streptococci were less susceptible (MIC ranges, 2-8 and 16-32 microg/mL, respectively). Fusidic acid activity against Gram-negative species was more limited (all MIC values, > or = 2 microg/mL) except for Empedobacter brevis, Moraxella catarrhalis and Neisseria meningitidis. A 4-fold increase in fusidic acid MIC results was observed when 10% serum was added to the broth. Decreasing medium pH to 5.0 to 6.0 negated the protein binding effects. Among the 8 antimicrobial combinations tested, gentamicin and rifampin enhanced the activity when combined with fusidic acid (no antagonism). Fusidic acid in vitro activity was most improved when combined with rifampin. Single-step mutational rates ranged from 1.2 x 10(-6) for 4x MIC to 9.8 x 10(-8) for 16 x MIC. In conclusion, these in vitro results for fusidic acid tested against contemporary strains confirm a persisting antimicrobial spectrum, especially against staphylococci and some other Gram-positive species. PMID:20159376

  7. The incidence and aetiology of hospitalised community-acquired pneumonia among Vietnamese adults: a prospective surveillance in Central Vietnam

    PubMed Central

    2013-01-01

    Background Lower respiratory tract infection (LRTI) including Community-acquired pneumonia (CAP) is a common infectious disease that is associated with significant morbidity and mortality. The patterns of aetiological pathogens differ by region and country. Special attention must be paid to CAP in Southeast Asia (SEA), a region facing rapid demographic transition. Estimates burden and aetiological patterns of CAP are essential for the clinical and public health management. The purposes of the study are to determine the incidence, aetiological pathogens, clinical pictures and risk factors of community-acquired pneumonia (CAP) in the Vietnamese adult population. Methods A prospective surveillance for hospitalised adult CAP was conducted in Khanh Hoa Province, Central Vietnam. All adults aged ?15 years with lower respiratory tract infections (LRTI) admitted to a provincial hospital from September 2009 to August 2010 were enrolled in the study. Patients were classified into CAP and non-pneumonic LRTI (NPLRTI) according to the radiological findings. Bacterial pathogens were identified from sputum samples by the conventional culture and polymerase chain reaction (PCR) for Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis; 13 respiratory viruses were identified from nasopharyngeal specimens by PCR. Results Of all 367 LRTI episodes examined, 174 (47%) were CAP. Older age, the presence of underlying respiratory conditions, and higher index score of smoking were associated with CAP. The one-year estimated incidence of hospitalised adult CAP in our study population was 0.81 per 1,000 person years. The incidence increased considerably with age and was highest among the elderly. The case fatality proportion of hospitalised CAP patients was 9.8%. Among 286 sputum samples tested for bacterial PCR, 79 (28%) were positive for H. influenzae, and 65 (23%) were positive for S. pneumoniae. Among 357 samples tested for viral PCR, 73 (21%) were positive for respiratory viruses; influenza A (n?=?32, 9%) was the most common. Conclusions The current adult CAP incidence in Vietnam was relatively low; this result was mainly attributed to the young age of our study population. PMID:23815298

  8. Monitoring Shifts in the Conformation Equilibrium of the Membrane Protein Cytochrome P450 Reductase (POR) in Nanodiscs*

    PubMed Central

    Wadsäter, Maria; Laursen, Tomas; Singha, Aparajita; Hatzakis, Nikos S.; Stamou, Dimitrios; Barker, Robert; Mortensen, Kell; Feidenhans'l, Robert; Møller, Birger Lindberg; Cárdenas, Marité

    2012-01-01

    Nanodiscs are self-assembled ?50-nm2 patches of lipid bilayers stabilized by amphipathic belt proteins. We demonstrate that a well ordered dense film of nanodiscs serves for non-destructive, label-free studies of isolated membrane proteins in a native like environment using neutron reflectometry (NR). This method exceeds studies of membrane proteins in vesicle or supported lipid bilayer because membrane proteins can be selectively adsorbed with controlled orientation. As a proof of concept, the mechanism of action of the membrane-anchored cytochrome P450 reductase (POR) is studied here. This enzyme is responsible for catalyzing the transfer of electrons from NADPH to cytochrome P450s and thus is a key enzyme in the biosynthesis of numerous primary and secondary metabolites in plants. Neutron reflectometry shows a coexistence of two different POR conformations, a compact and an extended form with a thickness of 44 and 79 ?, respectively. Upon complete reduction by NADPH, the conformational equilibrium shifts toward the compact form protecting the reduced FMN cofactor from engaging in unspecific electron transfer reaction. PMID:22891242

  9. Clinical Neisseria gonorrhoeae isolate with a N. meningitidis porA gene and no prolyliminopeptidase activity, Sweden, 2011: danger of false-negative genetic and culture diagnostic results.

    PubMed

    Golparian, D; Johansson, E; Unemo, M

    2012-01-01

    We describe a Neisseria gonorrhoeae strain, found in Sweden in 2011, that harbours a N. meningitidis porA gene causing false-negative results in PCRs targeting the gonococcal porA pseudogene. Furthermore, the strain had no prolyliminopeptidase (PIP) activity that many commercial biochemical kits for species verification in culture rely on. Enhanced awareness of the spread of such strains and screening for them can be crucial. PMID:22401563

  10. Shelf-life studies on carbon dioxide packaged golden croaker and speckled sea trout harvested from the Gulf of Mexico 

    E-print Network

    Banks, Harrell

    1979-01-01

    reported are: Pseudomonas, Acinetobacter/Moraxella, Vibrio, Flavobacterium, ~cb', M'*, Hulu, ~All', dP (Shewan, 1962; Nair and Lahiry, 1968). A shift in bacterial populations with changes in quality of fish has been postulated but has not been clearly...) were Pseudomonas, Acinetobacter, Flavobacterium, coryneform bacteria, Micrococcus, Moraxella, and Bacillus. The microflora of fresh croaker (Day 0) included Pseudo- monas, Plavobacterium, coryneform bacteria, Micrococcus, Moraxella, Bacillus...

  11. Qu es el Sistema Universidad Abierta y Educacin a Distancia (SUAyED)? En su origen, el Sistema Universidad Abierta se concibi como parte integral del proyecto de Reforma Universitaria impulsada por el

    E-print Network

    Islas, León

    ¿Qué es el Sistema Universidad Abierta y Educación a Distancia (SUAyED)? En su origen, el Sistema e innovadora en sus metodologías de enseñanza y evaluación de los conocimientos, con criterios de calidad y normados por un Estatuto aprobado por el Consejo Universitario el 25 de febrero de 1972, y por

  12. Ingeniería guiada por Modelado y Simulación de Eventos Discretos: Metodología y Caso de Estudio en la Red de Datos del Experimento ATLAS

    E-print Network

    Bonaventura, Matias Alejandro; The ATLAS collaboration

    2015-01-01

    En este trabajo presentamos una metodología iterativa e incremental para desarrollo de proyectos de ingeniería guiados por modelado y simulación (M&S) for-mal. Basándonos en el marco formal DEVS (Discrete Event Systems Specification), y sumando prácticas estándar en proyectos de software, se obtienen modelos que dan respuesta a preguntas de diseño y optimización de redes de datos. Presentamos una aplicación práctica de la metodología a un caso de estudio de ingeniería de redes, en el contexto de un experimento científico a gran escala: el experimento ATLAS (Máquina de Dios) en el cual científicos de todo el mundo intentan responder preguntas acerca del origen del universo. Estas redes absorben en tiempo real datos de experimentos físicos, y por su criticidad se someten a diseños exhaustivos y mejoras continuas. Sin embargo, por la escala del problema, estas redes están disponibles para ensayos solo esporádicamente. Mostraremos como asistimos su diseño mediante técnicas de modelado y s...

  13. Fuentes de variabilidad en el diagnóstico de gastritis atrófica multifocal asociada con la infección por Helicobacter pylori1

    PubMed Central

    Bravo, Luis Eduardo; Bravo, Juan Carlos; Realpe, José Luis; Zarama, Guillermo; Piazuelo, MarÍa Blanca; Correa, Pelayo

    2014-01-01

    RESUMEN Introducción El mapeo de las diferentes regiones del estómago y el número de fragmentos de mucosa gástrica disponibles para evaluación histopatológica son fuentes importantes de variación en el momento de clasificar y hacer la gradación de la gastritis crónica. Objetivos Estimar la sensibilidad del número de fragmentos de mucosa gástrica necesarios para establecer los diagnósticos de gastritis atrófica con metaplasia intestinal (MI), displasia y estado de infección por Helicobacter pylori. Además evaluar la variabilidad intra-observador en la clasificación de estas lesiones precursoras del cáncer gástrico. Materiales y métodos En una cohorte de 6 años de seguimiento se evaluaron 1,958 procedimientos de endoscopia realizados por dos gastroenterólogos. En cada procedimiento y de cada participante se obtuvieron 5 biopsias de mucosa gástrica que representaban antro, incisura angularis y cuerpo. Un único patólogo hizo la interpretación histológica de las 5 biopsias y proporcionó un diagnóstico definitivo global que se utilizó como patrón de referencia. Cada fragmento de mucosa gástrica examinado condujo a un diagnóstico individual para cada biopsia que se comparó con el patrón de referencia. La variabilidad intra-observador se evaluó en 127 personas que corresponden a una muestra aleatoria de 20% del total de endoscopias hechas a los 72 meses de seguimiento. Resultados La sensibilidad del diagnóstico de MI y displasia gástrica aumentó de manera significativa con el número de fragmentos de mucosa gástrica evaluados El sitio anatómico de mayor sensibilidad para el diagnóstico de MI y displasia fue la incisura angularis. Para descubrir H. pylori se logró alta sensibilidad con el estudio de un solo fragmento de mucosa gástrica (95.9%) y fue independiente del sitio de obtención de la biopsia. El acuerdo intra-observador para el diagnóstico de gastritis crónica fue 86.1% con valor kappa de 0.79 IC 95% (0.76-0.85). Las biopsias fijadas en alcohol fueron inadecuadas para demostrar H. pylori e interpretar los cambios de displasia. Conclusión El número de fragmentos de mucosa gástrica evaluados, el método de fijación y la región del estómago donde se obtienen las biopsias son factores muy importantes para lograr una correcta clasificación de la gastritis crónica atrófica multifocal. PMID:25267864

  14. Crystallization and preliminary X-ray analysis of the C-terminal fragment of PorM, a subunit of the Porphyromonas gingivalis type IX secretion system.

    PubMed

    Stathopulos, Julien; Cambillau, Christian; Cascales, Eric; Roussel, Alain; Leone, Philippe

    2015-01-01

    PorM is a membrane protein involved in the assembly of the type IX secretion system (T9SS) from Porphyromonas gingivalis, a major bacterial pathogen responsible for periodontal disease in humans. The periplasmic domain of PorM was overexpressed in Escherichia coli and purified. A fragment of the purified protein was obtained by limited proteolysis. Crystals of this fragment belonged to the tetragonal space group P4(3)2(1)2. Native and MAD data sets were recorded to 2.85 and 3.1?Å resolution, respectively, using synchrotron radiation. PMID:25615973

  15. La evolución de los conflictos por los recursos: Un análisis desde los tiempos de la colonia hasta el presente en América del Sur

    Microsoft Academic Search

    Gabriel De Paula

    La hipótesis de trabajo que se intentará demostrar en el presente trabajo es que las potencias por más de 500 años han implementado acciones tendientes al control sobre recursos naturales necesarios para el financiamiento y desarrollo de sus economías. A medida que avanza la historia y los avances tecnológicos las estrategias van modificándose, así como también los mecanismos de control,

  16. MEDIDAS ACORDADAS POR LA DEFENSORIA DE LOS DERECHOS UNIVERSITARIOS DE LA UNAM RELATIVAS A LA SUSPENSION DE LOS PROCEDIMIENTOS ACADEMICOS Y

    E-print Network

    Arroyo Rodríguez, Víctor

    MEDIDAS ACORDADAS POR LA DEFENSORIA DE LOS DERECHOS UNIVERSITARIOS DE LA UNAM RELATIVAS A LA Derechos Universitarios Dr. Leoncio Lara Sáenz con fundamento en las disposiciones contenidas tanto en el derechos y obligaciones de los estudiantes y miembros del personal académico en lo relativo a dichos

  17. DETERMINAÇÃO DA MIGRAÇÃO DO PENTACLOROFENOL (PCF) A PARTIR DE MADEIRA PARA ALIMENTOS POR ELETROFORESE CAPILAR E AVALIAÇÃO DO POTENCIAL DE TOXICIDADE

    Microsoft Academic Search

    Marco Antonio Mota; Vinicius Nery; Helena Zamith; Shirley Abrantes

    2007-01-01

    Os preservantes de madeira são todas as substâncias usadas para evitar o apodrecimento das madeiras por microrganismos, tornando-a resistente ao ataque e desenvolvimento de microrganismos xilófagos. Neste trabalho desenvolvemos uma metodologia analítica em eletroforese capilar capaz de determinar a migração desses preservantes de madeira a par- tir da madeira para os alimentos e estimar o seu potencial toxicológico. A metodologia

  18. Ante as noticias aparecidas nos medios de comunicacin as como os rumores que circulan por diversos mbitos, o Comit de Empresa do Persoal Laboral da

    E-print Network

    Fraguela, Basilio B.

    de alzada por parte do Comité ten como intención a axilización do proceso, pois non facelo suporía tarde, e algúns deles directamente no contencioso-administrativo. O recurso do Comité pretende que o non está interesado na dilación do proceso, polo que en ningún caso acudirá aos tribunais reclamando

  19. 2012 -2013Diploma SeniorPrograma A -Asignatura con actividades complementarias fuera del aula (viernes por la tarde o sbado todo el da)

    E-print Network

    Escolano, Francisco

    Embid Fernández Taller de Imagen de la UA 90642-1-F Resiliencia y regulación de las emociones resiliencia. Personalidad resistente. Estrategias de regulación emocional de las emociones negativas. Antonio Computación e Inteligencia Artificial C POR RAZONES DE FUERZA MAYOR, ESTE PROGRAMA PUEDE VERSE SUJETO A MO

  20. TABLN DE ANUNCIOS La recogida de DIPLOMAS de los cursos impartidos por este Departamento se harn slo los lunes y los

    E-print Network

    Escolano, Francisco

    TABLÓN DE ANUNCIOS La recogida de DIPLOMAS de los cursos impartidos por este Departamento se harán curso "WEB 2.0 APLICACIONES PARA LA INFORMACIÓN, LA COMUNICACIÓN Y EL APRENDIZAJE" (Publicada 12/05/2014) Del 8 al 21 de julio de 2014 (NO PRESENCIAL) Abierta la inscripción en el curso "BLOGS. UN RECURSO

  1. La Universidad de Alicante presenta los Cursos de Verano "Rafael La UA oferta 59 cursos marcados por la pluralidad, la diversidad de contenidos y la excelencia

    E-print Network

    Escolano, Francisco

    La Universidad de Alicante presenta los Cursos de Verano "Rafael Altamira" La UA oferta 59 cursos Universidad de Alicante ha presentado esta mañana los Cursos de Verano "Rafael Altamira" marcados por la, Manuel Palomar, quien ha dado a conocer los cursos en rueda de prensa, junto al Vicerrector de Cultura

  2. Applying New Technologies: ANT Automation es una empresa de Automatizacin Industrial, con operaciones en USA, Argentina y Espaa. La empresa est conformada por un slido grupo de

    E-print Network

    Maguitman, Ana Gabriela

    Applying New Technologies: ANT Automation es una empresa de Automatización Industrial, con más de 12 años de experiencia en Software/Automatización Industrial y por nuevos jóvenes talentos con el desarrollo de productos de software industrial: OPC, SCADA, MES, VISION ARTIFICIAL, MODELOS

  3. Indicadores de Riesgo de Desastre y de Gestión de Riesgo: Aplicación del Sistema de Indicadores a Bolivia 1980 ¿ 2000 (Indice de Déficit por Desastre)

    Microsoft Academic Search

    Luis Eduardo Yamín; Omar D. Cardona

    2006-01-01

    Esta presentación fue comisionada por la Red de Desastres Naturales del Diálogo Regional de Política para la VI Reunión Hemisférica celebrada los días 24 y 25 de abril de 2006. El propósito del sistema de indicadores desarrollado en el marco de la operación ATN\\/JF-7907-RG del BID, es dimensionar la vulnerabilidad y el riesgo, usando indicadores a escala nacional, para facilitar

  4. IEE 2310 Sistemas de Potencia 2 Semestre 2008 Fecha de entrega : Mircoles 24 de septiembre hasta las 18:00 por email a hrudnick@ing.puc.cl.

    E-print Network

    Rudnick, Hugh

    ello, se le solicita que analice la operación normal y una serie de casos particulares de dicho sistema en la figura y cuadros siguientes. Los estudios requeridos por los inversionistas y que usted como consultor debe resolver son: a. Estudio base de operación del sistema. Determine las condiciones de

  5. RACIONALIDADE AMBIENTAL POR PARTE DE PRODUTORES RURAIS SITUADOS NA REGIÃO DA NASCENTE DO RIO GRANDE Environmental racionality by the rural producers in the headwaters of the Rio Grande

    Microsoft Academic Search

    Anna Carolina; Salgado Jardim; Robson Amâncio; Marcos Affonso; Ortiz Gomes

    RESUMO Os objetivos deste artigo são identificar e caracterizar a racionalidade ambiental dos agricultores, por meio da visão de mundo, das percepções, dos conhecimentos e das práticas socioambientais em duas comunidades rurais situadas nas proximidades da nascente do Rio Grande, no município de Bocaina de Minas, MG. Especificamente, buscou-se caracterizar o significado e a importância que os recursos naturais têm

  6. Estudo dos níveis de exposição e efeitos à saúde por mercúrio metálico em uma população urbana de Poconé, Mato Grosso, Brasil

    Microsoft Academic Search

    Volney de M. Câmara; Alexandre P. Silva; Fátima Pivetta; Maurício A. Perez; Maria Imaculada M. Lima; Maria Izabel de F. Filhote; Lidia Maria B. Tavares; Marcos V. Maciel; F. V. Alheira; T. Dantas; M. S. Martins

    1996-01-01

    Resumo Foram avaliados os níveis de exposição e dos efeitos causados por emissões na atmosfe- ra de mercúrio metálico proveniente de casas compradoras de ouro na população do Município de Poconé, Estado de Mato Grosso. O projeto foi desenvolvido através da comparação da morbi- dade referida, dos exames clínicos e da análise dos teores de mercúrio na urina de três

  7. La inserción en el mercado laboral de los inmigrantes latinos en España y en los Estados Unidos: Diferencias por país de origen y estatus legal

    PubMed Central

    Connor, Phillip; Massey, Douglas

    2013-01-01

    Resumen Este artículo compara los resultados económicos entre los inmigrantes latinoamericanos en España y Estados Unidos. Detectamos un efecto de selección por el que la mayoría de los inmigrantes latinoamericanos en España proceden de Sudamérica de un entorno de clases medias, mientras la mayoría de los inmigrantes que van a los Estados Unidos son centroamericanos de clase baja. Este efecto de selección explica las diferencias transnacionales en la probabilidad de empleo, logro ocupacional y salarios obtenidos. A pesar de las diferencias en los orígenes y las características de los latinoamericanos en ambos países, los factores demográficos, humanos y de capital social parecen operar de forma similar en ambos países; y cuando los modelos se estiman separadamente por estatus legal, descubrimos que los efectos se acentúan más entre los inmigrantes irregulares cuando se los compara con los regulares, especialmente en Estados Unidos. PMID:24532857

  8. Corynebacterium jeikeium jk0268 constitutes for the 40 amino acid long PorACj, which forms a homooligomeric and anion-selective cell wall channel.

    PubMed

    Abdali, Narges; Barth, Enrico; Norouzy, Amir; Schulz, Robert; Nau, Werner M; Kleinekathöfer, Ulrich; Tauch, Andreas; Benz, Roland

    2013-01-01

    Corynebacterium jeikeium, a resident of human skin, is often associated with multidrug resistant nosocomial infections in immunodepressed patients. C. jeikeium K411 belongs to mycolic acid-containing actinomycetes, the mycolata and contains a channel-forming protein as judged from reconstitution experiments with artificial lipid bilayer experiments. The channel-forming protein was present in detergent treated cell walls and in extracts of whole cells using organic solvents. A gene coding for a 40 amino acid long polypeptide possibly responsible for the pore-forming activity was identified in the known genome of C. jeikeium by its similar chromosomal localization to known porH and porA genes of other Corynebacterium strains. The gene jk0268 was expressed in a porin deficient Corynebacterium glutamicum strain. For purification temporarily histidine-tailed or with a GST-tag at the N-terminus, the homogeneous protein caused channel-forming activity with an average conductance of 1.25 nS in 1M KCl identical to the channels formed by the detergent extracts. Zero-current membrane potential measurements of the voltage dependent channel implied selectivity for anions. This preference is according to single-channel analysis caused by some excess of cationic charges located in the channel lumen formed by oligomeric alpha-helical wheels. The channel has a suggested diameter of 1.4 nm as judged from the permeability of different sized hydrated anions using the Renkin correction factor. Surprisingly, the genome of C. jeikeium contained only one gene coding for a cell wall channel of the PorA/PorH type found in other Corynebacterium species. The possible evolutionary relationship between the heterooligomeric channels formed by certain Corynebacterium strains and the homooligomeric pore of C. jeikeium is discussed. PMID:24116064

  9. Adjuvant Effects Elicited by Novel Oligosaccharide Variants of Detoxified Meningococcal Lipopolysaccharides on Neisseria meningitidis Recombinant PorA Protein: A Comparison in Mice

    PubMed Central

    Mehta, Ojas H.; Norheim, Gunnstein; Hoe, J . Claire; Rollier, Christine S.; Nagaputra, Jerry C.; Makepeace, Katherine; Saleem, Muhammad; Chan, Hannah; Ferguson, David J. P.; Jones, Claire; Sadarangani, Manish; Hood, Derek W.; Feavers, Ian; Derrick, Jeremy P.; Pollard, Andrew J.; Moxon, E . Richard

    2014-01-01

    Neisseria meningitidis lipopolysaccharide (LPS) has adjuvant properties that can be exploited to assist vaccine immunogenicity. The modified penta-acylated LPS retains the adjuvant properties of hexa-acylated LPS but has a reduced toxicity profile. In this study we investigated whether two modified glycoform structures (LgtE and IcsB) of detoxified penta-acylated LPS exhibited differential adjuvant properties when formulated as native outer membrane vesicles (nOMVs) as compared to the previously described LgtB variant. Detoxified penta-acylated LPS was obtained by disruption of the lpxL1 gene (LpxL1 LPS), and three different glycoforms were obtained by disruption of the lgtB, lgtE or icsB genes respectively. Mice (mus musculus) were immunized with a recombinant PorA P1.7-2,4 (rPorA) protein co-administered with different nOMVs (containing a different PorA serosubtype P1.7,16), each of which expressed one of the three penta-acylated LPS glycoforms. All nOMVs induced IgG responses against the rPorA, but the nOMVs containing the penta-acylated LgtB-LpxL1 LPS glycoform induced significantly greater bactericidal activity compared to the other nOMVs or when the adjuvant was Alhydrogel. Compared to LgtE or IcsB LPS glycoforms, these data support the use of nOMVs containing detoxified, modified LgtB-LpxL1 LPS as a potential adjuvant for future meningococcal protein vaccines. PMID:25545241

  10. Binding of complement factor H to PorB3 and NspA enhances resistance of Neisseria meningitidis to anti-factor H binding protein bactericidal activity.

    PubMed

    Giuntini, Serena; Pajon, Rolando; Ram, Sanjay; Granoff, Dan M

    2015-04-01

    Among 25 serogroup B Neisseria meningitidis clinical isolates, we identified four (16%) with high factor H binding protein (FHbp) expression that were resistant to complement-mediated bactericidal activity of sera from mice immunized with recombinant FHbp vaccines. Two of the four isolates had evidence of human FH-dependent complement downregulation independent of FHbp. Since alternative complement pathway recruitment is critical for anti-FHbp bactericidal activity, we hypothesized that in these two isolates binding of FH to ligands other than FHbp contributes to anti-FHbp bactericidal resistance. Knocking out NspA, a known meningococcal FH ligand, converted both resistant isolates to anti-FHbp susceptible isolates. The addition of a nonbactericidal anti-NspA monoclonal antibody to the bactericidal reaction also increased anti-FHbp bactericidal activity. To identify a role for FH ligands other than NspA or FHbp in resistance, we created double NspA/FHbp knockout mutants. Mutants from both resistant isolates bound 10-fold more recombinant human FH domains 6 and 7 fused to Fc than double knockout mutants prepared from two sensitive meningococcal isolates. In light of recent studies showing functional FH-PorB2 interactions, we hypothesized that PorB3 from the resistant isolates recruited FH. Allelic exchange of porB3 from a resistant isolate to a sensitive isolate increased resistance of the sensitive isolate to anti-FHbp bactericidal activity (and vice versa). Thus, some PorB3 variants functionally bind human FH, which in the presence of NspA enhances anti-FHbp resistance. Combining anti-NspA antibodies with anti-FHbp antibodies can overcome resistance. Meningococcal vaccines that target both NspA and FHbp are likely to confer greater protection than either antigen alone. PMID:25644002

  11. The VR2 epitope on the PorA P1.7-2,4 protein is the major target for the immune response elicited by the strain-specific group B meningococcal vaccine MeNZB.

    PubMed

    Martin, D R; Ruijne, N; McCallum, L; O'Hallahan, J; Oster, P

    2006-04-01

    A protracted epidemic of group B meningococcal disease in New Zealand led to the testing of a strain-specific tailor-made vaccine, MeNZB. Immunogenicity levels achieved during age group trials enabled New Zealand's regulatory authority to grant licensure to deliver MeNZB to all individuals under age 20. During the trials target strains for serum bactericidal antibody measurements included the vaccine target strain NZ98/254 and two comparator epidemic-type strains (NZ94/167 and NZ02/09). In this study, 12 other strains differing variously from the vaccine strain by their capsular group, PorB type, and PorA variable region specificities, or PorA expression, were used as target strains. The PorA specificity of the serum bactericidal antibody responses to the vaccine was determined for 40 vaccinees. Sets of 10 pre- and postvaccination sera were chosen randomly from the young infant, older infant, toddler, and school-age group trials. Antibody recognition of linearized PorA proteins was also determined using immunoblotting. Across all age groups vaccine-induced serum bactericidal antibodies specifically targeted the VR2 P1.4 epitope of the PorA P1.7-2,4 protein irrespective of the PorB type and/or capsular type of the target strain. Deletion of amino acids within the VR2 epitope or replacement of the epitope through genetic exchange allowed strains variously to resist antibody-directed complement-mediated lysis and negated PorA-specific antibody recognition in immunoblots. The demonstration that the immunodominant antibody response was specifically for the VR2 P1.4 epitope of the PorA protein supports the public health decision to use a strain-specific vaccine for the control of New Zealand's epidemic of meningococcal disease. PMID:16603616

  12. Este curso, en ESPAOL, es un programa de capacitacin intensiva de 33 das (incluyendo 2 dias de viaje internacional a y de Colorado) organizado por el Centro para el Manejo y Capacitacin en reas Protegidas de la Universidad Estatal

    E-print Network

    viaje internacional a y de Colorado) organizado por el Centro para el Manejo y Capacitación en Áreas gestión, y tipos de uso. El programa incluye aproximadamente 70% del tiempo en el campo. Proporciona

  13. Four-month outbreak of invasive meningococcal disease caused by a rare serogroup B strain, identified through the use of molecular PorA subtyping, England, 2013.

    PubMed

    Chatt, C; Gajraj, R; Hawker, J; Neal, K; Tahir, M; Lawrence, M; Gray, S J; Lucidarme, J; Carr, A D; Clark, S A; Fowler, T

    2014-01-01

    Molecular PorA subtyping provides information that increasingly requires the adaptation of standard public health approaches to outbreak management. We report an outbreak of a rare subtype of meningococcal infection not previously identified in the United Kingdom (UK). The outbreak occurred in the Warwickshire area in England between February and June 2013. Molecular subtyping allowed the identification of additional cases, prompting an enhanced public health response that included efforts to identify potential social networks that might benefit from chemoprophylaxis. It also prompted swabbing to define nasopharyngeal carriage in the focal nursery and helped explain the unusual epidemiological pattern. Without subtyping to identify a link, the additional cases would have been managed as sporadic cases in accordance with current UK guidance. PMID:25394258

  14. Telithromycin. Aventis Pharma.

    PubMed

    Johnson, A P

    2001-12-01

    The ketolide telithromycin (HMR-3647; Ketek), a derivative of clarithromycin, has been launched by Aventis Pharma (formerly Hoechst Marion Roussel) for the treatment of respiratory tract infections with gram-positive or gram-negative cocci, Haemophilus influenzae, Streptococcus pneumoniae, Moraxella catarrhalis, intracellular pathogens, atypical microorganisms, toxoplasma or anaerobic bacteria. By May 2001, filings in the US and EU had been completed and a filing in Japan was expected to take place in the fourth quarter of 2001. In July 2001, telithromycin was granted marketing authorization by the EC for the treatment of community-acquired respiratory tract infections, including those caused by bacteria resistant to commonly used antibiotics. In October 2001, the product was launched in Germany. In March 2000, telithromycin was submitted to the US FDA and the EMEA, under the EU centralized approval procedure, for approval for the treatment of community-acquired pneumonia (CAP), acute sinusitis, acute exacerbations of chronic bronchitis and tonsillitis/pharyngitis. The company had expected to launch the product in early 2001. The CPMP issued a positive opinion for all four indications on April 23 2001. In September 2001, the company indicated that it expected the product to be launched in Japan in 2002. The FDA's Anti-infectives Advisory Committee was due to review telithromycin for all the submitted indications on January 29 2001; however, this was postponed. This postponement was thought to be at Aventis' request in order to discuss the potential for a resistant pneumococcal infection labeling which would boost product sales. The revised date for the meeting was April 26 2001, at which the Anti-Infective Drugs Advisory Committee of the FDA recommended approval of telithromycin for the treatment of CAP in patients 18 years of age or older. The committee failed to recommend approval for the use of the drug for the remaining three indications for which it was filed, citing concerns over potential cardiovascular risk and liver toxicity; at this time, the company was in active discussions with the FDA regarding approval of the remaining three indications. An approvable letter for CAP, acute bacterial exacerbations of chronic bronchitis and acute bacterial sinusitis was received by the company in June 2001; Aventis also received a non-approvable letter for the treatment of tonsillitis/pharyngitis at this time. In April 1999, ABN Amro predicted annual sales of DM 50 million in 2001, rising to DM 100 million in 2002. In February 1999, Lehman Brothers estimated a 70% probability that this ketolide would come to market. The analysts also estimated a launch date of 2001, with peak sales of US $700 million in 2009. Analysts Merrill Lynch predicted in September 200, that the product would be launched by 2001, with sales of euro 50 million in that year, rising to euro 284 million in 2004. Deutsche Bank predicted in August 2001, that sales of the product would reach euro 5 million in 2001, rising to euro 300 million in 2005. Analysts at Merrill Lynch predicted in November 2001, that the product would be resubmitted in the US in mid-2002, and would make sales of US $5 million in 2001, rising to US $250 million in 2004. PMID:11892930

  15. IgE Reactivity of Blue Swimmer Crab (Portunus pelagicus) Tropomyosin, Por p 1, and Other Allergens; Cross-Reactivity with Black Tiger Prawn and Effects of Heating

    PubMed Central

    Varese, Nirupama; Zubrinich, Celia; Lopata, Andreas L.; O'Hehir, Robyn E.; Rolland, Jennifer M.

    2013-01-01

    Shellfish allergy is a major cause of food-induced anaphylaxis, but the allergens are not well characterized. This study examined the effects of heating on blue swimmer crab (Portunus pelagicus) allergens in comparison with those of black tiger prawn (Penaeus monodon) by testing reactivity with shellfish-allergic subjects' serum IgE. Cooked extracts of both species showed markedly increased IgE reactivity by ELISA and immunoblotting, and clinical relevance of IgE reactivity was confirmed by basophil activation tests. Inhibition IgE ELISA and immunoblotting demonstrated cross-reactivity between the crab and prawn extracts, predominantly due to tropomyosin, but crab-specific IgE-reactivity was also observed. The major blue swimmer crab allergen tropomyosin, Por p 1, was cloned and sequenced, showing strong homology with tropomyosin of other crustacean species but also sequence variation within known and predicted linear IgE epitopes. These findings will advance more reliable diagnosis and management of potentially severe food allergy due to crustaceans. PMID:23840718

  16. FLP-FRT-Based Method To Obtain Unmarked Deletions of CHU_3237 (porU) and Large Genomic Fragments of Cytophaga hutchinsonii

    PubMed Central

    Wang, Ying; Wang, Zhiquan; Cao, Jing; Guan, Zhiwei

    2014-01-01

    Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover and FLP-FRT recombination system to obtain unmarked deletions of both single genes and large genomic fragments in C. hutchinsonii. Unmarked deletion of CHU_3237 (porU), an ortholog of the C-terminal signal peptidase of a type IX secretion system (T9SS), resulted in defects in colony spreading, cellulose degradation, and protein secretion, indicating that it is a component of the T9SS and that T9SS plays an important role in cellulose degradation by C. hutchinsonii. Furthermore, deletions of four large genomic fragments were obtained using our method, and the sizes of the excised fragments varied from 9 to 19 kb, spanning from 6 to 22 genes. The customized FLP-FRT method provides an efficient tool for more rapid progress in the cellulose degradation mechanism and other physiological aspects of C. hutchinsonii. PMID:25063660

  17. Adequação do Processo Plasma com Alimentação Automática de Arame para Recuperação de Superfícies por Soldagem Robotizada (Adaptation of the Plasma Process with Automatic Wire Feeding for Recovery of Surfaces by Automated Welding)

    Microsoft Academic Search

    Nelso Gauze Bonacorso; Jair Carlos Dutra

    2007-01-01

    Superfícies danifi cadas são normalmente recuperadas através de processos de soldagem, possuem na maioria das vezes, geometrias complexas e se localizam em posições desfavoráveis à soldagem. A soldagem robotizada é a alternativa natural por apresentar vantagens em relação ao processo manual, como: redução do tempo e do custo total da recuperação, melhor controle da geometria da superfície recuperada, melhor uniformidade

  18. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    ", organizado por el Consejo Español de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Políticas y educação básica. No caso brasileiro, considerando que o Estado é o responsável pela oferta e regulação da que o sistema educacional é um dos dispositivos de normalização das mudanças sociais (Santos, 1998: 2

  19. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    . Introducción Dentro del Sistema Interamericano, el reconocimiento de la importancia crucial de la libertad de informaciones y opiniones, y el de difundirlas, sin limitación de fronteras, por cualquier medio de expresión expresión. Este derecho comprende la libertad de buscar, recibir y difundir informaciones e ideas de toda

  20. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    América Latina que ven con preocupación cómo su capital humano se va erosionando. No obstante y por suficiente entre los especialistas como para servir de base: a) Que el capital humano, como cualquier otro tipo de capital, tiende a la concentración geográfica, fluyendo desde donde es menos abundante, hacia

  1. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Boyer, Edmond

    patrimonio natural y cultural y, por ende, la posibilidad de lograr el tan anhelado desarrollo sostenible LOCALES Y CONOCIMIENTOS TÉCNICO- CIENTÍFICOS PARA EL DESARROLLO SUSTENTABLE: ¿POSIBLE ALTERNATIVA AL miradas y posicionamiento teórico de este trabajo ya no se trataría de buscar un desarrollo alternativo

  2. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Paris-Sud XI, Université de

    , por ende, la posibilidad de lograr el tan anhelado desarrollo sostenible integral e inteligente de voluntaria, ayuda mutua, reciprocidad y desarrollo integral y sostenible entre todos los vecinos y/o familias este trabajo ya no se trataría de buscar un desarrollo alternativo, sino alternativas al desarrollo o

  3. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Boyer, Edmond

    ", organizado por el Consejo Español de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Políticas y CASO "PAPELERAS" Y LA ASAMBLEA CIUDADANA AMBIENTAL Jorgelina Sannazzar1 Resumen En la actualidad, y, incluyendo en algunos casos reclamos que configuran un nuevo escenario para el ejercicio de la ciudadanía

  4. Actas del Congreso Internacional "Amrica Latina: La autonoma de una regin", organizado por el Consejo Espaol de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Polticas y Sociologa de la Universidad Complutense de Madrid (UCM),

    E-print Network

    Boyer, Edmond

    a cabo y los estudios de casos focales realizados, se analizan las acciones de fortalecimiento y sus derechos. Para ello el estudio de casos1 representativos de una trayectoria histórico-política en este", organizado por el Consejo Español de Estudios Iberoamericanos (CEEIB) y la Facultad de Ciencias Políticas y

  5. IEE 2310 Sistemas de Potencia 1 Semestre 2007 Fecha de entrega : Da jueves 26 de abril hasta las 18:00 por email a hrudnick@ing.puc.cl.

    E-print Network

    Rudnick, Hugh

    ello, se le solicita que analice la operación normal y una serie de casos particulares de dicho sistema en la figura y cuadros siguientes. Los estudios requeridos por los inversionistas y que usted como consultor debe resolver son: a. Estudio base de operación del sistema. Determine las condiciones de

  6. Impacto da Rodovia Josil Espíndula Agostini (ES259) sobre a mor- talidade de animais silvestres (Vertebrata) por atropelamento2 The impact of the Josil Espíndula Agostini Road (ES249) on the mortality of wild animals (Ver- tebrata) by running over

    Microsoft Academic Search

    Marcela S. Milli; Marcelo Passamani

    Resumo O presente estudo determinou a mortalidade da fauna silvestre provocada por atropelamento na Rodovia Josil Espíndula Agostine, sendo que um trecho desta rodo- via permeia a Estação Biológica de Santa Lúcia. O trabalho teve início em maio de 2004 e se estendeu até dezembro do mesmo ano. Foram registradas 12 espécies atropeladas sendo aves a classe com maior representatividade.

  7. Evaluation of Neisseria gonorrhoeae Multiple-Locus Variable-Number Tandem-Repeat Analysis, N. gonorrhoeae Multiantigen Sequence Typing, and Full-Length porB Gene Sequence Analysis for Molecular Epidemiological Typing

    PubMed Central

    Golparian, Daniel; Bruisten, Sylvia M.; Schouls, Leo M.; Unemo, Magnus

    2012-01-01

    The performance characteristics of Neisseria gonorrhoeae multilocus variable-number tandem-repeat analysis were evaluated, by comparison with N. gonorrhoeae multiantigen sequence typing and full-length porB sequence typing. Assessment of the relatedness of intra- and interpatient isolates showed that all three genotyping techniques display a high resolution and typeability. PMID:22075591

  8. Evaluation of Neisseria gonorrhoeae multiple-locus variable-number tandem-repeat analysis, N. gonorrhoeae Multiantigen sequence typing, and full-length porB gene sequence analysis for molecular epidemiological typing.

    PubMed

    Heymans, Raymond; Golparian, Daniel; Bruisten, Sylvia M; Schouls, Leo M; Unemo, Magnus

    2012-01-01

    The performance characteristics of Neisseria gonorrhoeae multilocus variable-number tandem-repeat analysis were evaluated, by comparison with N. gonorrhoeae multiantigen sequence typing and full-length porB sequence typing. Assessment of the relatedness of intra- and interpatient isolates showed that all three genotyping techniques display a high resolution and typeability. PMID:22075591

  9. Shelf-life studies on carbon dioxide packaged golden croaker and speckled sea trout harvested from the Gulf of Mexico

    E-print Network

    Banks, Harrell

    1979-01-01

    dioxide as indicated by a change in pH and there was inhibition of increasing numbers of comtmon types of bacteria. Coyne (19323 ddd h th 9 h 2dh 9, 91 9, M' Bacillus, and Pseudomonas found on fish was inhibited by carbon dioxide but that certain other...) were Pseudomonas, Acinetobacter, Flavobacterium, coryneform bacteria, Micrococcus, Moraxella, and Bacillus. The microflora of fresh croaker (Day 0) included Pseudo- monas, Plavobacterium, coryneform bacteria, Micrococcus, Moraxella, Bacillus...

  10. De conformidad con la Ley de Derechos Humanos de 1977 del Distrito de Columbia, segn enmendada, Cdigo Oficial del Distrito de Columbia, Seccin 2-1401.01 et seq., (Ley), el Distrito de Columbia no discrimina, de forma visible ni percibida, por

    E-print Network

    Weaver, Harold A. "Hal"

    De conformidad con la Ley de Derechos Humanos de 1977 del Distrito de Columbia, según enmendada la ausencia, el empleado no perderá ninguno de sus beneficios de empleo, ni tampoco los derechos por las estipuladas por la Ley. LAS QUEJAS CON RESPECTO A ALGUNA DENEGACIÓN DE LOS DERECHOS EN VIRTUD DE

  11. 10/14/2014 Gracias, moscas, por la cerveza | Ciencia | EL PAS http://elpais.com/elpais/2014/10/10/ciencia/1412952158_666855.html 1/2

    E-print Network

    10/14/2014 Gracias, moscas, por la cerveza | Ciencia | EL PAÍS http://elpais.com/elpais/2014/10/10/ciencia/1412952158_666855.html 1/2 MIGUEL ÁNGEL CRIADO 14 OCT 2014 - 12:15 CEST 2 La levadura emite tres Ciencias naturales Ciencia El secreto de una buena cerveza no está en la cebada ni en el lúpulo sino en el

  12. The Understanding of Astronomy Concepts by Students from Basic Education of a Public School. (Spanish Title: El Entendimiento de Conceptos de Aastronmía Por Los Alumnos de Educación Básica en Una Escuela Pública.) O Entendimento de Conceitos de Astronomia Por Alunos da Educa&wcedil;ão Básica: O Caso de Uma Escola Pública Brasileira

    NASA Astrophysics Data System (ADS)

    Iria Machado, Daniel; dos Santos, Carlos

    2011-07-01

    We present the results obtained in a research on the comprehension of basic astronomical concepts, in which 561 students from fifth grade middle school to third grade high school of a public school of the city of Foz do Iguaçu (Brazil) took part. A test with 20 multiple-choice questions was applied to indentify the most common conceptions expressed by the students. This test was elaborated based on the literature about misconceptions and covered the following topics: the day-night cycle; the time zones; the seasons of the year; the phases of the Moon; the movement of the Moon; the apparent movement of the Sun in the celestial sphere; the eclipses; the dimensions and distances in the Universe; the brightness of the stars and its observation from Earth. Though a small progress was verified in the proportion of scientifically acceptable answers when comparing the eighth grade of middle school to the fifth, and the third grade of high school to the first, there was an overall predominance of alternative conceptions regarding most of the explored subjects, which persisted up to the last year of secondary school. The comparison to data found in this research made in other socio-cultural contexts revealed, in many aspects, similar notions and difficulties revealed by the students. Se presentan los resultados de una investigación sobre la comprensión de conceptos astronómicos básicos, en la cual participaron 561 estudiantes que cursaban entre el quinto grado de la enseñanza primaria y el tercer año de la enseñanza secundaria de una escuela pública de la ciudad de Foz do Iguaçu (Brasil). Se utilizó un test de 20 preguntas de opción múltiple para identificar las concepciones más comunes expresadas por los estudiantes. Este instrumento de recolección de datos se desarrolló en base a la literatura sobre las concepciones alternativas y trató los siguientes temas: el ciclo día-noche, los husos horarios, las estaciones del año, las fases de la Luna, el movimiento de la Luna, el movimiento aparente del Sol sobre la esfera celeste, los eclipses, las dimensiones y las distancias en el Universo, el brillo de las estrellas y su observación de la Tierra. Si bien ha habido un pequeño progreso en la proporción de respuestas aceptables científicamente cuando se cotejó el octavo grado de la enseñanza primaria con el quinto, y el último año de la enseñanza secundaria con el primero, se observó un predominio de las concepciones alternativas en relación a la mayoría de los temas explorados, que continuó hasta los últimos años de la educación básica. Una comparación con los datos encontrados en investigaciones realizadas en otros contextos socioculturales revelaron, en muchos aspectos, conceptos y dificultades similares expresadas por los estudiantes. Apresentam-se os resultados de uma investigação sobre a compreensão de conceitos astronômicos básicos, da qual participaram 561 estudantes da quinta série do Ensino Fundamental ao terceiro ano do Ensino Médio de uma escola pública da cidade de Foz do Iguaçu. Um teste com 20 questões de múltipla escolha foi aplicado para identificar as concepções mais comuns expressadas pelos alunos. Esse instrumento de coleta de dados foi elaborado com base na literatura sobre concepções alternativas e abordou os seguintes temas: o ciclo dia-noite; os fusos horários; as estações do ano; as fases da Lua; o movimento da Lua; o movimento aparente do Sol na esfera celeste; os eclipses; as dimensões e distâncias no Universo; o brilho das estrelas e sua observação da Terra. Embora se tenha verificado um pequeno progresso na proporção de respostas cientificamente aceitáveis ao se cotejar a oitava série do Ensino Fundamental com a quinta, e a terceira série do Ensino Médio com a primeira, houve o predomínio de concepções alternativas em relação à maior parte dos temas explorados, as quais persistiram até o último ano da Educação Básica. A comparação com dados encontrados em investigações realizadas em outros contextos socioculturais revelaram, em m

  13. Hospitalizaciones por EPOC en el paciente anciano

    Microsoft Academic Search

    Pere Almagro; Mónica Rodríguez-Carballeira; Kay Tun Chang; Verónica Romaní; Cristina Estrada; Bienvenido Barreiro; Josep Lluis Heredia; Jordi Mascaró

    2009-01-01

    IntroductionHospitalizations for decompensation of chronic obstructive pulmonary disease (COPD) mainly occur in the elderly. The aim of this study was to describe the characteristics of octogenarians admitted for COPD and to compare these characteristics with those in a younger group.

  14. TESIS DEFENDIDA POR Cynthia Beatriz Perez Castro

    E-print Network

    Fernandez, Thomas

    en Ciencias de la Computaci´on Dr. David Hilario Covarrubias Rosales Director de Estudios de Posgrado Julio del 2010 #12;CENTRO DE INVESTIGACI´ON CIENT´IFICA Y DE EDUCACI´ON SUPERIOR DE ENSENADA PROGRAMA DE al interior y al exterior. En ambos casos, el an´alisis del contenido de la imagen es requerido para

  15. Infección por Coxiella burnetii (fiebre Q)

    Microsoft Academic Search

    María Teresa Fraile Fariñas; Carlos Muñoz Collado

    2010-01-01

    In spite of being described over 60 years, Q fever is still a little known disease. The exact prevalence is also unknown, but probably the number of cases of Q fever is underestimated. There is much variation in the clinical presentation, including severe forms with a poor prognosis. Acute cases often present as an asymptomatic infection, flu-like syndrome, pneumonia or

  16. FITORREMEDIAÇÃO DE AQÜÍFEROS CONTAMINADOS POR GASOLINA

    Microsoft Academic Search

    FÁBIO NETTO MORENO; HENRY XAVIER CORSEUIL

    The purpose of this work was to investigate the ability of willow tree (Salix babylonica) in the vegetative uptake of ethanol and benzene as a preliminary study on phytoremediation. Hidroponic culture of willow cuttings in reactors dosed with ethanol and benzene revealed 99% reductions, for both contaminants. Such efficiency appears to be due not only to contaminant's uptake onto plant

  17. Manual do Usurio Comece por aqui.

    E-print Network

    e muito mais poderosas opções de pesquisa. Seja bem-vindo(a) à nova experiência de pesquisa da ProQuest. O novíssimo ambiente de pesquisa da ProQuest, além de ser poderoso, abrangente e fácil de navegar, reúne recursos da ProQuest, Cambridge Scientific Abstracts (CSA) e Chadwyck-HealeyTM. É a melhor maneira

  18. Planificacin de Saneamiento Ambiental Urbano liderado por

    E-print Network

    Wehrli, Bernhard

    social y ambientalmente sostenibles con el objetivo de proporcionar una vivienda adecuada para todos. Los, generación de capacidades y financiamiento para vivienda y desarrollo urbano. www.unhabitat.org / www

  19. Infecciones causadas por el género Malassezia

    Microsoft Academic Search

    R. Arenas

    Summary The yeast from the gender Malassezia sp, previously called Pityrosporum are found as part of the microbiota of the skin and 92 to 100% of them have been isolated from healthy people. The taxonomy of the gender has been controversial since it was named M. furfur. These opportunistic yeasts are related with some human and animal (mamifers) diseases and

  20. Microbiological characteristics of Pacific shrimp (Pandalus jordani).

    PubMed Central

    Lee, J S; Pfeifer, D K

    1977-01-01

    Microorganisms associated with Pacific shrimp (Pandalus jordani) were isolated and identified. Those on the iced raw shrimp, which yielded an average count of 1.6 x 10(6), were predominantly Moraxella, Pseudomonas, Acinetobacter, Arthrobacter, and Flavobacterium-Cytophaga spp. The blanching and peeling reduced the microbial level to 3.3 x 10(4) and also selectively eliminated Moraxella spp. The microbial flora changed after each processing sequence, and the heat sensitivity and growth characteristics of the representative microbial groups suggested that the presence of Arthrobacter and Acinetobacter spp. in peeled shrimp may indicate inadequate cleaning of raw shrimp or a shorter blanching time. The presence of Moraxella and Flavobacterium-Cytophaga spp. would indicate the degree of secondary contamination, and the presence of Pseudomonas spp. would indicate the shelf-age of the processed shrimp. PMID:869532

  1. O que se Traz para a Vida e o que a Vida nos Traz: Uma Análise da Equação Etiológica proposta por Freud à Luz das Neurociências What we Bring to Life and what Life Brings to Us: An Analysis of the Ethiological Equation proposed by Freud According to Neurosciences

    Microsoft Academic Search

    Monah Winograd; Carlos A. Q. Coimbra; J. Landeira-Fernandez

    Resumo O presente trabalho tem como objetivo discutir aspectos teóricos, clínicos e experimentais acerca da interação entre variáveis inatas e aprendidas com relação à origem e desenvolvimento de distúrbios emocionais. Apresenta- se inicialmente a equação etiológica proposta por Freud acerca das neuroses na virada do século XX e sua relação com a teoria da sedução. Apresentam-se também algumas evidências clínicas

  2. Comparative efficacy and safety evaluation of cefaclor VS amoxycillin + calvulanate in children with Acute Otitis Media (AOM)

    Microsoft Academic Search

    Mukesh Aggarwal; Ramanuj Sinha; M. Vasudeva Murali; Prita Trihan; P. K. Singhal

    2005-01-01

    Acute Otitis Media (AOM) is the most frequent respiratory tract infection of infancy and childhood that is treated with antimicrobial\\u000a agents. The most common causative pathogens includeStreptococcus pneumoniae, Hemophilus influenzae andMoxarella catarrhalis, and therefore antibacterial management should target against these isolates. Cefactor, a congener of cephalexin monohydrate,\\u000a is a semisynthetic cephalosporin antibiotic. It is an orally active cephalosporin which has

  3. Infección por virus de la inmunodeficiencia humana y hepatitis víricas

    Microsoft Academic Search

    Vicente Soriano; Luz Martin-Carbonero; Eugenia Vispo; Pablo Labarga; Pablo Barreiro

    Hepatic complications currently represent one of the leading reasons for medical consultations, hospitalisation, and death in the HIV-infected population. This is due to a large extent to viral hepatitis, given its disproportionate frequency in this population. Chronic hepatitis B affects 5-10% of the HIV-infected population. Vaccination has reduced the incidence of liver disease related to hepatitis-B virus (HBV), and the

  4. Necrólisis epidérmica tóxica por olanzapina: reporte de un caso clínico

    Microsoft Academic Search

    Eduardo Contreras Zúñiga; Sandra Ximena; Zuluaga Martínez; Vanesa Ocampo

    Introduction: In 1956, Alan Lyell used the term toxic epidermal necrolysis (TEN) to descri- be a histological and clinical pattern characterized by necrosis of the epidermis, diffuse epidermolysis with exfoliation and\\/or training bulosas injuries, as well as non- cutaneous manifestations such as hydro electrolytic, thermoregulatory and catabolic impairment. Ob- jective: To describe a clinical case with a larger and unusual

  5. Por: Miguel Lpez OCTAVA REUNIN NACIONAL DE PERCEPCIN

    E-print Network

    Gilbes, Fernando

    ocurra el desastre. · ¿ Qué Tipo de data hidrológica está disponible en formato shapefile? Observed DATA (SHAPEFILE & GOOGLE EARTH-KML) · CAPAS DE POLÍGONOS Y PUNTOS #12;#12;· Creación de tres (3) tablas para extraer la data necesaria de los dos (2) shapefiles descargados. (Nov_6_2010, Nov_7_2010 y Total

  6. POR QUE LA INFERENCIA ESTADISTICA M. A. Gomez Villegas

    E-print Network

    Gómez Villegas, Miguel Ángel

    ~no para contrastar la hip´otesis nula H0 : = 0 frente a la hip´otesis alternativa H1 : = 0 para una tambi´en en funci´on del p-valor, o probabilidad bajo la hip´otesis nula del suceso cola observado, as que si ´este es menor que 0.05 o 0.01 o 0.001 se rechaza la hip´otesis nula, en primer lugar ¿no es

  7. Honduras Celebrates the IV Festival de Teatro por la Paz

    E-print Network

    Cohen, Deb; Stone, Kent

    1992-10-01

    . Known in Honduras as the movement of the Método de la Basura, from Reyes' theatrical treatise of that title, the peasant theatre movement normally operates in workshops conducted in campesino communities as consciousness-raising exercises in the oral..., Moisés Landaverde, who was assassinated for his activism in promoting popular theatre in 1987. Some of the group names revealed their origins or affiliations: Las Varas, Escuela Normal Pedro Nunfio, Actores del Lago, Pastoral Universitaria. Others...

  8. Control de las Enfermedades Transportadas por el Aire

    NSDL National Science Digital Library

    Wladyslaw Kowalski (Pennsylvania State Universityâ??s Indoor Environment Center; )

    2009-08-19

    The air that you breathe indoors may be dangerous to your health. How can we make indoor environments safer? Apply aerobiological engineering principles to buildings to control the bioaerosols in our indoor environments. Retrofit old buildings or specifically design new buildings to control airborne microbes. Develop regulatory standards for indoor environments. Become educated about sources and transmission routes of airborne pathogens.

  9. PROTECTION DES ROBOTS MOBILES POR TILISATION EN ATMOSPHERE EXPLOSIVE

    E-print Network

    Boyer, Edmond

    Thc utilization of clectrical apparatus in hazardous arcas requires thc using of tricd technics explosive atmosphcre in thc vicüüty ofignition capablc parts, the suppression of dangerous sparks they arc integrated at thc beginning of the projcct. High powcr accumulators shall be protccted

  10. Computando lo aspero Por que se cuelgan las computadoras?

    E-print Network

    Figueira, Santiago

    construir una m´aquina que demuestre teoremas y as´i encuentre todas las verdades matem´aticas. Kurt G¨odel´aquina no puede resolver. #12;Historia de Hilbert y sus amigos David Hilbert: Bueno... yo pens´e que... Kurt G¨odel

  11. Tratamiento y prevención del síndrome de neurotoxicidad inducido por opioides

    Microsoft Academic Search

    C. CENTENO; E. BRUERA; Grey Nuns

    1999-01-01

    Close attention to patients treated with morphine or drugs derived from it can accelerate the diagnosis of the Opioid Induced Neurotoxicity Syndrome (OIN). It may allow the application of some therapeutic measu- res that provide hopeful results. Rotation of the type of opioid that had been used in combination with suitable hydration have been proved to be an efficient treatment

  12. DESGASTE POR DESLIZAMIENTO EN SECO DE ACEROS PERLÍTICOS Y BAINÍTICOS

    Microsoft Academic Search

    CRISTIAN CAMILO; VIÁFARA ARANGO

    Sliding wear has a great influence on the performance of rail -wheel systems, especially because the wheel flange slides over the rail in a curve track. Since rail-wheel sliding introduces adhesive effects, high slip ratios strongly affect the rolling contact fatigue wear acting on the surfaces. Sliding wear tests were carried out in a Pin-on-Disk device to study the performance

  13. Enseñanza de la ingeniería de software por procesos instrumentados

    Microsoft Academic Search

    Rubby Casallas; Jaime I. Davila; Juan P. Quiroga

    In this paper we present a Software Engineering course that combines in an integrated and balanced way: processes, methodology and support tools to develop a software product. We are called our approach \\

  14. Por el cual se establecen las polticas de Bienestar Universitario.

    E-print Network

    Prieto, Germán A.

    concebido como un aporte al proceso educativo mediante acciones intencionalmente formativas que permitan el- tes, docentes-investigadores y personal administrativo), teniendo en #12;432 cuenta la diversidad de

  15. O PRÍNCIPE PROPOSTO POR NICOLAU MAQUIAVEL NO SÉCULO XVI

    Microsoft Academic Search

    Marcia Aparecida; Lopes Benassi

    The present communication has the purpose of making some considerations about the political thought of the Italian Niccolo Machiavelli, author who lived between the 15th and 16th centuries, a period of rapid decomposition of the feudal order and also of fights for the construction of the modern institutions. Differently from countries like France and Spain, for example, that at this

  16. Clasificacin de Msica por Gnero utilizando Redes Neuronales Artificiales

    E-print Network

    Gao, Guang R.

    repercusión que tiene la aplicación de AdaBoost sobre este clasificador débil. 1 INTRODUCCIÓN Bases de datos base de datos y finalmente se exponen los resultados obtenidos haciendo énfasis en las diferencias que investigación LAME [12]. La base de datos consta de 500 canciones, 90% para entrenamiento y 10% para evaluación

  17. Sostenibilidad y desarrollo sostenible: un modelo por construir

    Microsoft Academic Search

    Josep Antequera; Ernesto González; Leonardo Ríos Osorio

    1970-01-01

    El present article pretén donar una visió del desenvolupament sostenible com a marc de referència -conceptual i pràctic- el desenvolupament del qual s'emmarca dintre dels processos cognitius de l'ésser humà. En aquest sentit, les idees que donen forma als conceptes de desenvolupament sostenible i sostenibilitat es podrien veure com un model d'explicació i visualització del binomi Home-Natura, que intenta, al

  18. Tese de doutorado apresentada por: Diego Pinheiro Aguiar

    E-print Network

    mesencéfalo pela crista neural cefálica: regulação de Foxg1 pelas vias de sinalização Bmp e Wnt Tese de dout mésencéphale par la crête neurale cephalique: regulation de l'expression de Foxg1 par les voies de

  19. Diagnóstico de criptococose canina pela citologia aspirativa por agulha fina

    Microsoft Academic Search

    Danieli Brolo Martins; Anna Laetícia Trindade Barbosa; Ayrton Cavalheiro; Sonia Terezinha dos Anjos Lopes; Janio Morais Santurio; João Eduardo Schossler; Alexandre Mazzanti

    2008-01-01

    This paper aimed to described a 1.5 year-old Labrador male, diagnosed with cryptococcosis using fine-needle aspiration cytology (FNAC). The dog was showing signs of depression and hyporexia. Peripheral lymph nodes and a mass situated between the submandibular lymph nodes were aspirated. The cytology showed yeast-like structures resembling Cryptococcus sp. in all samples examined. These findings were confirmed by culture (C.

  20. Diagnóstico de criptococose canina pela citologia aspirativa por agulha fina

    Microsoft Academic Search

    Danieli Brolo; MartinsI Anna; Laetícia Trindade; Sonia Terezinha; Janio Morais

    This paper aimed to described a 1.5 year-old Labrador male, diagnosed with cryptococcosis using fine-needle aspiration cytology (FNAC). The dog was showing signs of depression and hyporexia. Peripheral lymph nodes and a mass situated between the submandibular lymph nodes were aspirated. The cytology showed yeast-like structures resembling Cryptococcus sp. in all samples examined. These findings were confirmed by culture (C.

  1. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    PubMed Central

    Gómez-Duarte, Oscar G.

    2014-01-01

    Resumen Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratorios clínicos de centros de salud. Estudios recientes han reportado la detección de E. coli patógenas intestinales en Colombia, siendo la E. coli enterotoxigénica la cepa más frecuentemente asociada a diarrea en niños menores de 5 años. Otros patógenos detectados en estos pacientes incluyen las E. coli enteroagregativa, enteropatógena, productora de toxina Shiga, y de adherencia difusa. Con base en estudios que reportan la presencia de E. coli productora de toxina Shiga y E. coli enteroagregativa en carnes y vegetales en supermercados, se cree que productos alimentarios contaminados contribuyen a la transmisión de estos patógenos y a la infección del huésped susceptible. Más estudios son necesarios para evaluar los mecanismos de transmisión, el impacto en la epidemiologia de la EDA, y las pautas de manejo y prevención de estos patógenos que afectan la población pediátrica en Colombia. PMID:25491457

  2. Predação de Opisthocomus hoazin por Spizaetus ornatus e de Bubulcus ibis por Bubo virginianus em Tocantins, Brasil

    Microsoft Academic Search

    Túlio Dornas; Renato Torres Pinheiro

    Predation of Cattle Egret by the Great Horned Owl and Hoazin by the Ornate Hawk-Eagle in western Tocantins State, Brazil. We report the predation of Hoazin Opisthocomus hoazin by an immature Ornate Hawk-Eagle Spizaetus ornatus, a new prey for this species in Brazil. The Ornate Hawk-Eagle roosted together with Hoazin assembly in branches of Sapium haematospermum, typical river borderline vegetation.

  3. Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species

    Microsoft Academic Search

    Josa-Marie Wehrfritz; Jon P. Carter; Stephen Spiro; David J. Richardson

    1997-01-01

    Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera Pseudomonas, Moraxella, Arthrobacter and Aeromonas. A hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the Pseudomonas and Aeromonas spp. and in total soluble fractions of the Arthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the Pseudomonas species and

  4. The microbial flora of pond-reared shrimp (Penaeus stylirostris, P. setiferus, P. vannamei, and Macrobrachium rosenbergii) 

    E-print Network

    Christopher, Frank Mitchell

    1976-01-01

    to their initial level af- 2 ter 21-22 days and continued to increase with counts at 28 days ranging from 10 -10 per gram. Although Aeromonas 6 7 and Pseudomonas species continued to dominate the microbial flora after 7 days, Acinetobacter and Pseudomonas... the principal isolates from the drip. After 6 days storage, Moraxella-Acinetobacter, Micrococcus, and Cor ebacterium were the predominant genera. After 10 days, Pseudomonas dominated the microbial flora of the drip. These studies illustrate the relationship...

  5. Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater

    Microsoft Academic Search

    G. W. Fuhs; Min Chen

    1975-01-01

    Several strains resembling members of theAcinetobacter-Moraxella-Mima group of bacteria were isolated from activated sludge-type sewage treatment plants designed for phosphate removal. The bacteria are obligate aerobes but utilize as carbon and energy sources low-molecular intermediates generated anaerobically, particularly acetate and ethanol. These bacteria can be shown to be responsible for the phosphate luxury uptake occurring in these treatment plants. The

  6. Effect of crude extracts of selected actinomycetes on biofilm formation of A. schindleri, M. aci, and B. cereus.

    PubMed

    Saleem, Hafiz Ghulam Murtaza; Aftab, Usman; Sajid, Imran; Abbas, Zaigham; Sabri, Anjum Nasim

    2015-05-01

    Actinomycetes are well known group of gram positive bacteria for their potential to produce antibiotics. This study sought to assess the ability of the selected actinomycetes to control biofilm forming bacteria isolated from different dental plaque samples. On the basis of morphological differences three out of ten different dental plaque bacterial isolates were selected for further study. These isolates were biochemically and genetically characterized and were identified as Acinetobacter schinndleri, Moraxella aci, and Bacillus cereus. Antibiotic resistant profile was measured through disc diffusion method and found that all three isolates were moderately sensitive to ofloxacin and erythromycin and resistant to trimethoprim. Antibacterial activity of ten different Streptomyces strains was assessed through an agar plug and well diffusion method against three dental biofilm forming bacteria. Two Streptomyces strains named as S. erythrogriseus and S. labedae showed good antibacterial activity against Moraxella and Acinetobacter strains. Ability of the four active antibiotic producing strains to inhibit biofilm formation was assessed using microtiter biofilm detection assay. It was found that biofilm forming ability of Acinetobacter and Moraxella was inhibited by S. labedae an antibiotic producing strain, while S. macrosporeus can only inhibit biofilm formation by B. cereus. PMID:25138589

  7. Posibilidades y limitaciones de la gestión por resultados de salud, el pago por objetivos y el redireccionamiento de los incentivos. Informe SESPAS 2008

    Microsoft Academic Search

    Salvador Peiró; Anna García-Altés

    2008-01-01

    The concept of pay-for-performance (P4P) encompasses different strategies that aim to stimulate health care quality improvement by remunerating healthcare providers according to their performance in specific measures of efficiency or quality. Although the effectiveness of P4P in improving quality of care is largely unknown, these systems are being widely adopted in the United Kingdom, the United States and other countries,

  8. trad. em portugus (Brasil) por Olival Freire, rev. tc. por Sylvia Gemignani Garcia, Tempo Social (USP, So Paulo), 11, n1-maio, 1999, 67-73.

    E-print Network

    Paris-Sud XI, Université de

    , philosophical, social) reflection on science starting from the elementary levels of scientific education. Résumé social), Pinochet garantiu estabilidade política e social durante 17 anos ao custo de repressão, terror

  9. [Participation in causing O.M.E. with nasopharyngeal alpha-Streptococcus].

    PubMed

    Fujimori, I; Goto, R; Hisamatsu, K; Nozawa, I; Ogino, J; Murakami, Y; Kikushima, K; Yamada, T

    1995-09-01

    The role of normal pharyngeal flora in the defense mechanism against infections in the upper respiratory tract was studied in 50 children with otitis media with effusion (O.M.E.). In the bacteriological study of the nasopharynx, the incidence of H. influenzae, S. pneumoniae, S. aureus, M. catarrhalis and group A Streptococcus was about 46%, 24%, 20%, 12% and 8%, respectively. The incidence of these species in the cases with O.M.E. was higher than that in the cases with chronic tonsilitis or control cases. In 41 O.M.E. cases with alpha-streptococci (82%), the incidence of alpha-streptococci with inhibitory activity against 5 pathogens (H. influenzae, S. pneumoniae, S. aureus, M. catarrhalis, group A Streptococcus) was examined. The detection rate of alpha-streptococcal strains with inhibitory activity against 5 pathogens derived from the nasopharynx in the cases with O.M.E. was significantly lower than that of the strain in the chronic tonsilitis cases and the control cases. Moreover, the detection rate of inhibitory alpha-streptococci from the nasopharynx was lower than that of from the tonsil. These findings suggest that the decline of inhibitory activity against pathogens by normal flora in nasopharynx is one of the factors causing O.M.E. PMID:7594799

  10. The effect of marbling and subcutaneous fat on the microbiological and sensory characteristics of beef strip loin steaks 

    E-print Network

    Correale, Karen Kross

    1986-01-01

    58. 2 0. 4 88. 9 56. 7 24. 8 10. 9 31. 9 Plavobacterium 5. 6 Pseudomonas 1. 3 6. 5 11. 1 Hafnia alvei 16. 6 B. ~lt t Noraxella-Acinetobacter 20. 0 16. 7 2. 7 16. 7 25. 4 0. 9 Alcaligenes 16. 7 1. 6 Table 4. Continued Percentage.... 8 47. 3 76. 0 47. 4 Leuconostoc spp. Flavobacterium 0. 5 11. 0 6. 7 12. 2 Pseudomonas 1. 0 13. 8 0. 3 Nafnia alvei 0. 4 0. 6 S. liquefaciens Moraxella-Acinetobacter 22. 2 34. 9 2. 2 16. 8 3. 7 Alcaligenes 0. 4 18 Additional Tests...

  11. A microbiological examination of deep muscle tissue of beef, pork and lamb carcasses 

    E-print Network

    Nickelson, Ranzell, II

    1969-01-01

    ) 96. 4 8(10) 69. 2 87. 8 Micrococcus 3. 8 Sarcina S~t. t Coryneforms Bacillus Clostridium Flavobacter ium 3 (2) 19. 2 3. 8 6. 1 1. 2 Pseudomonas Moraxella Alcaligenes Acinetobacter anitratum (H 11 1 1 ~ 8 3. 8 1 2 1. 2 Miscellaneous..., Alcaligenes cold 3 warm 20 cold 20 20 b Staphylococcus, I'~iicrococcus Bacillus 4 warm cold 20 Bacillus 5 warm cold 6 warm cold N. D. d 20 NOD Coryneform N D 7 warm cold 8 warm 80 cold 9 warm cold N. D. 40 20 120 N. D Coryneform...

  12. Biodegradation of Tributyltin (TBT) by Extremophile Bacteria from Atacama Desert and Speciation of Tin By-products.

    PubMed

    Yáñez, Jorge; Riffo, Paula; Santander, Paola; Mansilla, Héctor D; Mondaca, María Angélica; Campos, Víctor; Amarasiriwardena, Dulasiri

    2015-07-01

    Biodegradation of tributyltin (TBT) by four tin resistant Gram negative bacteria isolated from extremely contaminated river sediments in the Atacama Desert in Chile was studied. Moraxella osloensis showed the greatest resistance and degradation capability of TBT, producing less toxic by-products, such as dibutyltin (DBT) and inorganic tin. In 7 days, approximately 80 % of TBT degradation was achieved, generating close to 20 % of DBT as degradation product. The degradation rate constant (k) was 0.022 [day(-1)] and TBT half-life (t1/2) in culture was 4.3 days. Debutylation is stated a probable mechanism of TBT degradation. PMID:25975619

  13. Microbiological assessment of critical control points and safety of precooked beef products 

    E-print Network

    Michel, Michael Ellis

    1990-01-01

    -30 d at 2-7'C (23). Other bacteria that were reported to be present as part of the microflora of fresh vacuum- packaged beef are Leuco?ostoc spp. , Brochothnx thermospltacta, Aeromonas hydrophila, Pseudomonas spp. , Streptococcus spp. , Moraxella-Acinetobacter... be exercised over one or more factors which, if controlled, could minimize or prevent a hazard (14). There are two types of critical control points (CCP): CCP1 and CCP2. CCP I's are those areas that, if monitored carefully, will assure control of a hazard...

  14. Characterization of some fish and shrimp spoiling bacteria

    Microsoft Academic Search

    Kitty J. A. van Spreekens

    1977-01-01

    The classification of some important groups of bacteria involved in fish and shrimp spoilage was studied.\\u000a \\u000a Trimethylamine is produced byPseudomonas putrefaciens, a “non-defined” group resemblingPs. putrefaciens, Photobacterium spp. and someMoraxella-like bacteria.\\u000a \\u000a \\u000a \\u000a Hypoxanthine is produced by the same groups of bacteria except the last named and also by the “typical shrimp spoilers” (presumptiveAlteromonas).\\u000a \\u000a \\u000a \\u000a Strong off-odours are produced on fresh fish byPs.

  15. ESTUDIO CINETICO DE LA DEGRADACION DE LA ACTIVIDAD ANTIOXIDANTE HIDROSOLUBLE DE JUGOS CITRICOS POR TRATAMIENTO TERMICO

    Microsoft Academic Search

    Belén ACEVEDO; Mabel MONTIEL; Jorge AVANZA

    In this work we have studied the degradation of Antioxidant Activities by thermic treat- ment at 70, 80 y 90ºC in juices of varios citrus: orange (Citrus sinensis), mandarins (Citrus reticu- lata), lemon (Citrus limon), pomelo (Citrus paradisi) y lime Rangpur (Citrus limonia Osbeck). Antioxidant Activities were measured using the DPPH method. Antioxidant Activities degradation follows a zero-order reaction.

  16. Prof. Sylvio Canuto, IFUSP O processo de submisso, editorao e reviso por

    E-print Network

    Souza, Max O.

    of the Chemical Physics Letters (2003-2009) ·Specialist Editor of the Computer Physics Communications (1987 of Physical Chemistry A e B · Journal of Physical Chemistry Letters · Journal of Molecular Liquids · Journal and Computational Chemistry · Physical Chemistry and Chemical Physics (PCCP) · Physical Review A, B, E · Physical

  17. 11.02.04.99.13 Saldo de Vagas (por Curso) UNIVERSIDADE FEDERAL DO PARAN

    E-print Network

    Paraná, Universidade Federal do

    15 140 1BT013 - FARMACOLOGIA I / Turma: A 15 110 4BT013 - FARMACOLOGIA I / Turma: C 15 140 1BT013 - FARMACOLOGIA I / Turma: B 15 50 10BT013 - FARMACOLOGIA I / Turma: D 15 140 1BT014 - FARMACOLOGIA II / Turma: A 15 120 3BT014 - FARMACOLOGIA II / Turma: B 15 70 8BT014 - FARMACOLOGIA II / Turma: C 15 40 11BT014

  18. TOXICIDAD OFTALMOLÓGICA SEVERA POR TOPAMAX SEVERE OCULAR SIDE EFFECTS WITH TOPAMAX

    Microsoft Academic Search

    ASENSIO-SÁNCHEZ VM; TORREBLANCA-AGÜERA B; MARTÍNEZ-CALVO S; CALVO MJ; RODRÍGUEZ R

    Case reports: We describe 2 patients who develo- ped ocular side effects during treatment with topira- mate. One was a 16-year-old woman with generali- zed seizures who developed a homonymous hemia- nopia after using topiramate 125 mg per day for 12 weeks, and the other was a 24-year-old epileptic female who developed a bilateral maculopathy after using topiramate 150 mg

  19. 16 FEBRUARY 2005 hree in the morning, too much coffee, and hours of por-

    E-print Network

    Brown, Ezra

    . They began building the bell tower in the 1170s, but stopped after putting up only three stories when, with the pepper, leather, and fur trades and so many people coming and going. . .a great place to grow up in

  20. Várices por insuficiencia de la vena perforante poplítea. Nuestra experiencia en el diagnóstico y tratamiento quirúrgico

    Microsoft Academic Search

    Jorge A. Pozzi

    Introduction: Until recently, popliteal perforator vein insufficiency was not considered as a source of varicose veins. The study of its diagnosis and treatment must be considered by the vascular surgeon to avoid varicose recurrences at the popliteal region. Objectives: To show the results of the diagnostic and therapeutic measures in limbs with popliteal perforator vein insufficiency and its complications. Patients

  1. "Por Los Ojos De Madres": Latina Mothers' Understandings of College Readiness

    ERIC Educational Resources Information Center

    Cortez, Laura Jean; Martinez, Melissa Ann; Sáenz, Victor B.

    2014-01-01

    In this study, data from six focus groups with 30 Latina mothers in South Texas were analyzed utilizing a "funds of knowledge" approach to uncover their understandings of college readiness and their role in ensuring their children are college ready. Findings indicate that Latina mothers perceived college readiness in a holistic fashion,…

  2. Mapeamento da cobertura vegetal natural e antrópica do bioma Cerrado por meio de imagens Landsat ETM

    Microsoft Academic Search

    Edson Eyji Sano; Roberto Rosa; Jorge Luís; Silva Brito; Laerte Guimarães Ferreira; Heleno da Silva Bezerra

    The Cerrado biome occupies 204,7 million hectares in the central part of Brazil. Mapping its land cover from satellite remote sensing is challenging because of its geographic extension, marked seasonality, highly dynamic and intensive land occupation, and spectral confusion between some natural and anthropogenic classes (e.g., natural grasslands and cultivated pastures). The goal of this study was to produce 1:250,000

  3. DAÑOS OCASIONADOS POR VERTEBRADOS SILVESTRES AL CULTIVO DE MAÍZ EN LA SELVA LACANDONA, CHIAPAS, MÉXICO

    Microsoft Academic Search

    Karina G. Romero-Balderas; Eduardo J. Naranjo; Helda H. Morales; Ronald B. Nigh

    SUMMARY The purpose of this study was to identify wild vertebrate spe- cies causing damage in corn crops at the Lacandon Forest of Chiapas, Mexico, and to estimate and compare losses in corn plots with different traits, such as water availability, dead trees, shape, slope, sowing date, distance to towns, and presence of adjacent corn crops. Twenty-two corn plots were

  4. Utilização dos recursos naturais por comunidades humanas do Parque Ecoturístico do Guamá, Belém, Pará1

    Microsoft Academic Search

    Ana Sílvia; Sardinha RIBEIRO; Maria das Dores; Correia PALHA; Manoel Malheiros TOURINHO; PALAVRAS CHAVE

    This work shows data about the use of natural resources by the human population located in (n=1) and around (n=6) the Guamá Ecotouristic Park (GEP), in order to evaluate the impact on the local fauna and flora in view of the project for constructing a road through the park. The methodology utilized in this community study was the application of

  5. Interact: um modelo de interao para interfaces 2D por manipulao direta MARCELO MEDEIROS CARNEIRO

    E-print Network

    -900 Rio de Janeiro, RJ, Brasil {mmc,gattass,levy,enio}@tecgraf.puc-rio.br 2 IPRJ/UERJ - Instituto Politécnico, Campus Regional da UERJ Rua Alberto Rangel s/no , 28601-970 Nova Friburgo, RJ, Brasil mmc. Miguez de Mello Cidade Universitária, Quadra 7, Sala 9020, 21949-900 Rio de Janeiro, RJ, Brasil enio

  6. Home | Search | Employee Locator | Visitor Info | Library | Espaol | Site Overview Enfermedades provocadas por el

    E-print Network

    Bezrukov, Sergey M.

    algunas ideas de cómo prevenir esas enfermedades y de cómo cuidarse. Alergias y asma En Estados Unidos ataques repentinos que los dejan sin aliento y respirando con dificultad. Esto se llama asma alérgica. Ataques de asma muchas veces ocurren después de períodos de mucho ejercicio o cuando las condiciones

  7. ESTRATEGIAS DE AFRONTAMIENTO DE CRISIS CAUSADAS POR DESEMPLEO EN FAMILIAS CON HIJOS ADOLESCENTES EN BOGOTÁ

    Microsoft Academic Search

    ERNESTO MARTÍN PADILLA; CARLOS FAJARDO GONZÁLES; ADRIANA GUTIÉRREZ MORALES; DANIEL PALMA PRIETO

    2007-01-01

    The objective of this article is to describe and understand coping strategies for facing a crisis caused by unemployment in families with adolescent children in the city of Bogota. The conceptual framework for the study is the systemic approach that facilitates a relational and profound understanding of the problems dealt with. The social interpretative approach, based on the principles of

  8. La coleccin del Centro de Documentacin Estadstica est formada por estadsticas espaolas, estadsticas

    E-print Network

    Autonoma de Madrid, Universidad

    interno. Consumidores 13.01.03 Creación de empleo. Desempleo 02.03 Seguridad social 06.04 Establecimientos empleo. Contratos de desempleo, Invalidez, etc. 07.01 Economía agraria consumidor, de la energía. 13

  9. Diferencias en el desempleo por género. ¿ Hay discriminación en el acceso al trabajo?

    Microsoft Academic Search

    Mauricio Amador Pilonieta; Paula Herrera Idárraga

    2006-01-01

    Esta investigación analiza la iferencia existente entre las tasas de desempleo de hombres y mujeres en Colombia. Se observa que la tasa de desempleo de la mujer siempre ha sido superior a la del hombre y que en los últimos años la diferencia ha disminuido. Se busca establecer cuáles son las características de la mujer que hacen que su tasa

  10. ÁNALISIS DE LAS PRIMAS POR PLAZO EN EL MERCADO ESPAÑOL DE DEUDA PÚBLICA

    Microsoft Academic Search

    Paz Rico Belda

    1998-01-01

    This paper analyses the term premia in the Spanish public debt market over the period from January of 1991 to December of 1995. The purpose is to determine the hypothesis that explains the relationship between short-term and long-term interest rates in the Spanish public debt market. We obtain empirical evidence of time varying term premia, so we can conclude that

  11. UNIVERSIDAD DE PUERTO RICO PLAN MEDICO DE ESTUDIANTES CONTRATADO POR TRIPLE S SALUD

    E-print Network

    Quirk, Gregory J.

    MAJOR MEDICAL CUBIERTA BÁSICA CON DENTAL CUBIERTA BÁSICA CON MAJOR MEDICAL CUBIERTA BÁSICA CON DENTAL Y MAJOR MEDICAL CUBIERTA BÁSICA CON FARMACIA Y MAJOR MEDICAL 1er SEMESTRE MEDICAL CUBIERTA BÁSICA CON DENTAL CUBIERTA BÁSICA CON MAJOR MEDICAL CUBIERTA BÁSICA CON

  12. Inhibición de vibrios patógenos de camarón por mezclas de cepas probióticas

    Microsoft Academic Search

    María Auxiliadora Sotomayor; José Luis Balcázar

    2003-01-01

    Summary Inhibition of shrimp pathogenic vibrios by mixture of probiotic strains A series of probiotic strain were challenged using an in vitro inhibition test with three pathogenic vibrios (Vibrio harveyi E22, V. vulnificus S2 and V. parahaemolyticus PA2) in order to confirm its antagonistic effect. The mixtures P63-Ili, P62-P64 and P62-P63-Ili presented a percentage of inhibition greater than 50%. This

  13. Qualidade de vida e dimensão ocupacional na esquizofrenia: uma comparação por sexo

    Microsoft Academic Search

    Clareci Silva Cardoso; Waleska Teixeira Caiaffa; Marina Bandeira; Arminda Lucia Siqueira; Mery Natali Silva Abreu; José Otávio Penido Fonseca

    2006-01-01

    Schizophrenia has been associated with low quality of life in patients, and the impact can vary by gender. Knowing gender differences may help implement specific interventions. This study focuses on quality of life in male and fe- male outpatients with schizophrenia, particu- larly examining the occupational domain. A cross-sectional study using the Quality of Life Scale (QLS-BR) was carried out.

  14. Doctrina cristiana para instrucción e información de los indios, por manera de hystoria 

    E-print Network

    Có rdoba, Pedro de, 1482-1521 Doctrina cristiana para instrucció n e informació n de los indios, por manera de hystoria  Có rdoba, Pedro de, 1482-1521 Analysis of clerical operation by application of work sampling  Springer, Joy Victor

    1954-01-01

    praotioes?nd avoidable del?ys, An investigation of tbs reliability and validity of the retie-delay metbcd vas sc?de by D, S, Carrell? Industrial Engineer for Deere and Company? and R+ N? Barnes, Professor of Engineering at the Cniversity of C... ~ 1954 during the period of oloseoat, This oloseont operation is a seasonal operation lasting about sixty days Da te Pay- 1 roll 2 3 4 Clc se 5 out 6 7 9 10 "r- 11 riv- 12 ing 13 14 De- 15 part-16 iiig 17 18 'ub 19 Doc 20 0th- 21...

  15. Chemotherapy induced neuropathy in clinical practice Neuropatía inducida por quimioterapia en la práctica clínica

    Microsoft Academic Search

    Camilo E. Fadul

    SUMMARY Neuropathic pain is a common symptom amongst patients with chemotherapy induced peripheral nerve neurotoxicity, and symptoms are indistinguishable from those seen with toxic neuropathy from other causes. The neuropathy is predominantly sensory and follows a glove-stocking distribution and associates with burning pain. Autonomic neuropathy manifested as constipation frequently occurs. There are some risk factors for neuropathy caused by chemotherapy:

  16. Determinação da pressão de vapor de compostos orgânicos por cromatografia gasosa

    Microsoft Academic Search

    Fabrício P. Povh; Vera M. Rodrigues; Maria A. A. Meireles; Nanci Pinheiro

    2006-01-01

    SUMMARY DETERMINATION OF VAPOR PRESSURE OF ORGANIC COMPOUNDS BY GAS CHROMATOGRAPHY. The knowledge of the vapor pressures of natural compounds, as well as their critical properties are of great interest for the application of supercritical extraction and supercritical impregnation dye, and necessary for the thermodynamic modeling of equilibria phase. The scarcity of experimental data for these compounds results from their

  17. El Titulo IX y La Discriminacion por Sexo (Title IX and Sex Discrimination).

    ERIC Educational Resources Information Center

    Office for Civil Rights (ED), Washington, DC.

    Title IX of the Education Amendments of 1972 protects people from discrimination based on sex in education programs or activities that receive Federal financial assistance. This brochure outlines the responsibilities of education programs and activities covered by Title IX, the responsibilities of the Office for Civil Rights (OCR) in enforcing…

  18. El Libro de la Escritura por Pinguino Tinto (The Writing Book, by Inky Penguin).

    ERIC Educational Resources Information Center

    Padgett, Ron

    Presented completely in Spanish and intended for elementary level students, this book offers 12 writing ideas and several suggestions on how students can make a book using their writing. Each writing idea is presented with a brief description (addressed to the student), several examples of student writing, and a blank page on which to write.…

  19. Ensenando El Espanol por Medio de Accion (Teaching Spanish through Action).

    ERIC Educational Resources Information Center

    Segal, Bertha

    A teaching guide containing 102 elementary to intermediate level Spanish lessons is presented. The lessons are based on the Total Physical Response technique of second language teaching. They follow the stages of first language acquisition: listening, speaking, and reading. Each of the ten units contains a list of new vocabulary words, individual…

  20. Producción de Nuez en Nogales (Carya illinoensis (Wangenh.) K. Koch) Atacados por Phymatotrichopsis omnivora (Duggar) Hennebert

    Microsoft Academic Search

    José Alfredo Samaniego-Gaxiola; Teodoro Herrera-Pérez

    Nut production was compared in healthy and diseased pecan trees with cotton root rot (Phymatotrichopsis omnivora), established under different management and soil conditions. Healthy and diseased pecan trees with different severity were selected from orchards la Barranca, Florida, and Florencia, to evaluate nut production during three years for the first orchard, and for four years in the other two. Significant

  1. INSTRUMENTOS DE RESPONSABILIDAD SOCIAL EMPRESARIAL VISTOS POR ORGANIZACIONES NO GUBERNAMENTALES, ONG

    Microsoft Academic Search

    María G. Daza

    2010-01-01

    La investigación se fundamenta en una revisión documental y lectura analítica de los archivos, publicaciones y declaraciones de cuatro Organizaciones No Gubernamentales (ONG) quienes expresan su posición sobre la efectividad de algunos de los Códigos de Conducta más conocidos. El estudio clasifica inicialmente los Códigos de Conducta en dos grupos: privados y públicos, con el fin de identificar la procedencia

  2. Renovando la Esperanza por una Educacion sin Exclusiones (Rekindling the Hope for an Education without Exclusion).

    ERIC Educational Resources Information Center

    Revista Interamericana de Educacion de Adultos, 2001

    2001-01-01

    Articles in this issue, written in Spanish, focus on the following: current status and outlook of youth and adult education; opening statement of the 50th anniversary commemoration; regional framework for the education of youth and adults in Latin America and the Caribbean; interculturalism and the education of youth and adults; participation of…

  3. El cancer de pulmon como marcador de tabaquismo: relacion con la mortalidad por cancer no pulmonar

    Microsoft Academic Search

    Monica Perez-Rios; Bruce Leistikow; Agustin Montes

    Objective: To assess the possible role of tobacco smoke in non-lung cancer (excluding stomach cancer) using changes in lung cancer mortality rates as a proxy for tobacco exposure. Methods: A time series analysis of cancer mortality was performed to evaluate the possible association between changes in mortality rates for lung cancer and for non-lung, non-stomach cancer (NLNS) from 1970 to

  4. Mortalidad por defectos del tubo neural en México, 1980-1997

    Microsoft Academic Search

    José A Ramírez-Espitia; Fernando G Benavides; Marina Lacasaña-Navarro; José M Martínez; Ana M García; Joan Benach

    2003-01-01

    Objective. To describe the mortality due to neural tube defects (NTD) in Mexico for the 1980-1997 period. Mate- rial and Methods. The annual NTD mortality rates per 10000 liveborn infants were calculated by state and for the country. The time trend was evaluated with the annual per- cent change (APC) obtained using a Poisson regression model. The NTD mortality ratio

  5. Medición del patrón ventilatorio mediante tomografía por impedancia eléctrica en pacientes con EPOC

    Microsoft Academic Search

    Marco Balleza; Núria Calaf; Teresa Feixas; Mercedes González; Daniel Antón; Pere J. Riu; Pere Casan

    2009-01-01

    Background and ObjectiveThe measurement of breathing pattern in patients with chronic obstructive pulmonary disease (COPD) by electrical impedance tomography (EIT) requires the use of a mathematical calibration model incorporating not only anthropometric characteristics (previously evaluated in healthy individuals) but probably functional alterations associated with COPD as well. The aim of this study was to analyze the association between EIT measurements

  6. Reacción granulomatosa facial por rellenos cosméticos inyectados: presentación de cinco casos

    Microsoft Academic Search

    Rafael Poveda; José V. Bagán; Judith Murillo; Yolanda Jiménez; Rafael Poveda Roda

    The use of substances to augment soft tissues as aesthetic purpose is associated with, among other undesirable effects, the appearance of foreign body granulomas. The improvements made to these substances have reduced the incidence of adverse reactions, but not eliminated them. We present five cases of foreign body reactions to three different products, dimethylpolysi- loxane (silicone), bovine collagen, and polylactic

  7. Lista de alumnos por tribunales (actualizado 21-7-2014) Informacin para los alumnos

    E-print Network

    Rey Juan Carlos, Universidad

    , Salvador Elaboración de productos periodísticos con apoyo documental. El uso de las fuentes para reportajes. José Luis Rodríguez Grado en Periodismo Fuenlabrada 05321552-L Barranco García, Andrea Puedes ser tú. La solidaridad como consecuencia de la crisis en Madrid. José Luis Martín Sáez Doble Grado en

  8. Monitoramento da contaminação por elementos traço em ostras comercializadas em Recife, Pernambuco, Brasil

    Microsoft Academic Search

    André Dias Cavalcanti

    2003-01-01

    Samples of oysters marketed in Recife, Pernambuco, Brazil, were monitored for the concentration of trace elements (Hg, Zn, Fe, Cu, and Mn) for one year (from March 2001 to Feb- ruary 2002). Mercury was the principal contaminant found in oysters and the element posing the greatest public health risk. Mercury levels in oysters reached 551.12µg\\/kg (wet weight). These values suggest

  9. DESINFECCIÓN SOLAR DE AGUAS POR FOTÓLISIS Y FOTOCATÁLISIS: APLICACIÓN EN TUCUMÁN, ARGENTINA

    Microsoft Academic Search

    M. Litter; Energía Atómica

    Solar Water Disinfection (SODIS) and Solar Heterogeneous Photocatalysis (FHS) are effective, inexpensive and easy to use methods in places that lack water mains and have scant resources. This work reports studies carried out to validate SODIS and FHS technologies for bacterial disinfection. In the latter case, TiO2 was used as a catalyst on different supports like PET bottles and porcelain

  10. Producao d Dijatos por Dupla Troca de Pomeron Exclusiva no Experimento D0

    SciTech Connect

    Rangel, Murilo, Santana; /Rio de Janeiro, CBPF

    2008-10-01

    The first search for exclusive diffractive dijet production with invariant mass {approx}> 100 GeV in Run II of the Fermilab Tevatron Collider is performed. The set of data used is the Run IIa, corresponding to an integrated luminosity of 30 pb{sup -1} of p{bar p} collisions at {radical}s = 1.96 TeV taken with the D0 detector. At 95% CL, an upper limit for the ratio between the number of diffractive exclusive events and the number of non diffractive events is set to be 7.5 x 10{sup -6}, excluding two of the three models proposed to explain this production.

  11. Índices de estacionalidad de los precios medios recibidos por los productores de manzanas chilenas

    Microsoft Academic Search

    Germán Lobos Andrade; Tristán Muñoz Ibáñez

    2005-01-01

    The purpose of this paper is to analyze the behavior of FOB (free on board) average prices for the Chilean fresh apples. Based on this analysis, the average prices received by farmers were estimated. The patterns of seasonally adjusted price fluctuations, received by Chilean apple farmers, were estimated through average mobile geometric method. Estimates considered monthly average FOB prices from

  12. El efecto invernadero producido por el CO2 atmosférico: una nueva interpretación termodinámica

    Microsoft Academic Search

    EDUARDO R ABRIL

    The glasshouse effect caused by atmospheric CO2: a new thermodynamic interpretation: At present times the use of models to simulate and predict the climate change and its consequences on global ecological processes has contributed to a better understanding of the phenomena in several disciplines. However, there is evidence that current models fail to simulate a number of well known climatic

  13. DESENVOLVIMENTO DE LIGAS FERROSAS PARA SOLDA DE REVESTIMENTO DURO AUXILIADO POR SIMULAÇÕES TERMODINÂMICAS

    Microsoft Academic Search

    Edmilson Otoni Correa; Nelson Guedes de Alcântara; Dorival Gonçalves Tecco; Ramachandran Vasant Kumar

    A thermodynamic model is used to investigate the solidification of a hardfacing material in Fe-Cr-C-Nb system and, developed for cladding components subjected to severe abrasive wear by welding. Microstructural characterization of the alloy showed that the theoretical simulations carried out to predict the amount and volume fraction of the phases using the module Scheil of MT-DATA Software presented very realistic

  14. Buprenorfina en el manejo de dolor por cáncer The Use of Buprenorphine to Control Cancer Pain

    Microsoft Academic Search

    María Catalina; Soto Niño

    Opiods are the current pharmalogical remedies of choice for the control of moderate to severe pain. Subsequently, it is important to rely on a number of prescriptions and applications for these drugs, particularly in the case of oncological patients. Low molecular weight, high potency and liposolubility of buprenorphine combine with its favorable tolerance and low abuse probability. Clinical evidence reveals

  15. A PERCEPÇÃO DOS CARPINTEIROS SOBRE A ORGANIZAÇÃO DO SEU TRABALHO CONSTATADA POR MAPAS COGNITIVOS

    Microsoft Academic Search

    José Adelino; Leila Amaral

    This article deals with the carpentry tasks at construction sites, applying the ergonomic analysis of the work. The study intended to verify the efficiency of an education process. The carpentry tasks were analyzed, including the implications, the conditioning factors and the work organization. The activities performed by the carpenters were observed, and a photographic register showed all the required steps.

  16. Por um conjunto de princípios que possibilitem a construção de novos modelos de sistemas de informação

    Microsoft Academic Search

    Marlei Pozzebon; Henrique M. R. de Freitas

    This paper presents the challenge that leads GESID's researchers to try to achieve new information systems models. The research aims mainly to identify what are the rules or principles, that allow us to design each part of on information system. (GESID: Information and Decision System Research Group)

  17. Comercialização de carqueja por ervateiros da zona central de Pelotas, Rio Grande do Sul

    Microsoft Academic Search

    Gustavo Heiden; Leila Macias; Vera Lucia Bobrowski; João Ricardo Vieira Iganci

    RESUMO A venda de carqueja (Baccharis L. seção Caulopterae DC., Asteraceae), é uma atividade comum entre ervateiros da zona central de Pelotas, bem como a procura e uso desta planta pelos consumidores. Com o objetivo de caracterizar a comercialização e o consumo de carqueja, foram entrevistados ervateiros da Zona Central da cidade de Pelotas, localizada no Sul do Estado do

  18. ¿Por Qué un Banco Público de Células Madre de Sangre de Cordon Umbilical en Colombia?

    Microsoft Academic Search

    JULIETA HENAO PÉREZ; EUGENIA CRISTINA; PACHECO PINEDO; GERMÁN DAVID; ARBOLEDA TORO; GÓMEZ ARISTIZABAL; LUZ MARINA; RESTREPO MÚNERA

    2005-01-01

    El trasplante de células madres hematopoyéticas (CMH) obtenidas de médula ósea (MO) se ha convertido en el tratamiento de elección para diferentes desórdenes hematológicos. Pero éste presenta dificultades, que pueden mejorarse con el uso de de sangre de cordón umbilical (SCU), fuente importante de CMH de rápida disponibilidad, fácil obtención, inofensivo para el donante, menor restricción de histocompatibilidad (HLA), menor

  19. Emisiones en el interior de las viviendas por la combustión de carbón en los hogares

    Cancer.gov

    Quemar carbón dentro de las viviendas para calefacción o preparación de alimentos produce emisiones de partículas y gases que pueden contener una cantidad de sustancias químicas dañinas, como benceno, monóxido de carbono, formaldehído e hidrocarburo aromático policíclico.

  20. Proceso de Evaluación de Desempeño por Competencias en una Empresa Financiera Privada de Lima Metropolitana

    Microsoft Academic Search

    Jiza Zárate Amayo

    El presente estudio se enmarca dentro de la investigación aplicada. Se evaluó a 453 trabajadores de una empresa financiera divididos en dos grupos ocupacionales: administrativos (n=426) y ejecutivos (n=27) que comprenden once gerencias cada uno. El instrumento midió nueve competencias: Eficiencia del Trabajo, Conocimientos del Trabajo, Iniciativa y Creatividad, Efectividad en la Solución de Problemas, Sensibilidad hacia el Cliente, Trabajo

  1. CARACTERIZACIÓN ESPECTROSCÓPICA DE ACEROS MEDIANTE LA TÉCNICA PLASMA INDUCIDO POR LÁSER (LIBS)

    Microsoft Academic Search

    E. Jaimes; R. Cabanzo

    Laser-Induced Breakdown Spectroscopy is a technique where a pulsed laser beam focused on a sample, produce ablation of small quantities of material and formation of plasma. Spectroscopy analysis of the radiation emitted by this plasma, allows detection of the elements in the sample and determination its concentration. LIBS is an efficient analytic technique for the traces analisys of samples in

  2. Um Sistema de Pesagem de Aves por Viso Computacional EDUARDO VEGAS CONEJO

    E-print Network

    broiler weighing system based on computer vision is described. In the implementation, the weight is correlated statistically with linear measurements taken from reference points in the broiler body profile

  3. Um Sistema de Pesagem de Aves por Viso Computacional EDUARDO VEGAS CONEJO 1

    E-print Network

    broiler weighing system based on computer vision is described. In the implementation, the weight is correlated statistically with linear measurements taken from reference points in the broiler body profile

  4. IMPLEMENTAÇÃO E ANÁLISE DE LOCALIZAÇÃO EM ROBÓTICA MÓVEL POR FILTRO DE KALMAN

    Microsoft Academic Search

    Claudiney Calixto da Silva; Juliano Augusto Pereira; Praça Marechal Eduardo Gomes

    This paper presents the verification of continuous positioning estimation for a mobile robot using four sensors (three sonars and one odometer). These estimations are performed by a computationally efficient algorithm, with recursive formulation called Kalman Filter. The robot has the capability of moving on static environments, knowing its initial position and all environment obstacles. According to this information, the Kalman

  5. Estimaciones de la prevalencia del trastorno por déficit de atención-hiperactividad en niños marabinos

    Microsoft Academic Search

    M. López; D. Pirela; J. J. Cardozo

    Summary. Aims. This study reports the findings of research aimed at determining the rate of prevalence of attention deficit hyperactivity disorder (ADHD) in children of school age. Subjects and methods. The epidemiological study was conducted using a community sample extracted by means of multi-stage stratified sampling according to socio-economic level and schooling and consisted in 1,141 children of both sexes

  6. 93PARCELAS DE AGRADO ALREDEDOR DE SANTIAGO Y VALPARASO. MIGRACIN POR AMENIDAD A LA CHILENA?

    E-print Network

    Borsdorf, Axel

    a su origen- destino, temporalidad y al grupo humano implicado. Durante las últimas décadas se están cercanas a la capital (San José: 1 millón de habitantes), a unos 10 km. Stewart (2002) analiza también las

  7. Marea roja producida por Lingulodinium polyedrum (Peridiniales, Dinophyceae) en Bahía Culebra, Golfo de Papagayo, Costa Rica

    Microsoft Academic Search

    Alvaro Morales-Ramírez; Roxana Víquez; Karina Rodríguez; Maribel Vargas

    2001-01-01

    This is the first record of the dinoflagellate Lingulodinium polyedrum in a red tide bloom in the North Pacific coast of Costa Rica. The sample was collected on April 2000 at Culebra Bay, Gulf of Papagayo, from a patch of aproximatly 2000 m 2 , which produced a red discoloration of the water and a peculiar strong odor. This species

  8. José González Dávila: Réquiem por un amigo que inicia su leyenda

    E-print Network

    Mijares, Enrique

    2000-10-01

    , estuvieron salpicadas de humor, un humor negro, ácido, macabro si se quiere, pero que nos sirvió para exorcizar la inminencia de la muerte. El hablaba de estar dispuesto a fabricar su propio mito, como Frida Kalho, decía. Hablaba de presentarse en el...

  9. AVALIAÇÃO DA APROPRIAÇÃO CONCEITUAL NO ENSINO DE CIÊNCIAS POR MEIO DE ATIVIDADES DE EDUCAÇÃO AMBIENTAL

    Microsoft Academic Search

    Maria Elízia Pacheco Ferreira; Rosely Aparecida; Liguori Imbernon; Escola de Artes; Miriam Aparecida Romano

    2009-01-01

    The present study developed with students from 6ª degree (7º year) of basic education, considers activities\\/interventions that can precede environment studies and environmental education (EE). The proposal configures as strategy that makes possible more interaction with the environment and an evident construction of relations between the scientific knowledge and the world. The methodological referential research-action is adopted by the researchers,

  10. Prevalencia de sucesos violentos y de trastorno por estrés postraumático en la población mexicana

    Microsoft Academic Search

    Ma. Elena Medina-Mora; Guilherme Borges-Guimaraes; Carmen Lara; Luciana Ramos-Lira; Joaquín Zambrano; Clara Fleiz-Bautista

    2005-01-01

    Objective. To report the rate of exposure to different vio- lent events, their demographic correlates, the prevalence of Post-Traumatic Stress Disorder (PTSD), and the impact on quality of life. Material and Methods. The National Sur- vey of Psychiatric Epidemiology is representative of the Mexican urban population aged 18 to 65. The survey was undertaken in 2001 and 2002 using the

  11. Datos epidemiológicos del trastorno por déficit de atención con hiperactividad en una muestra de niños marabinos

    Microsoft Academic Search

    J. A. Peña

    Summary. Aims. This study reports the findings of a research project aimed at determining the rate of prevalence of attention deficit hyperactivity disorder (ADHD) in school-aged children. Patients and methods. Different behavioural and cognitive measures were administered to a sample of children between 3 and 13 years of age obtained by means of a multistage sampling procedure that was stratified

  12. INTEGRACI ´ ON POR CUANTIFICACI ´ ON DE SISTEMAS STIFF. PARTE I: TEORIA

    Microsoft Academic Search

    Ernesto Kofman; Gustavo Migoni

    Este art´ iculo presenta un nuevo metodo de integracion numerica de ecuaciones diferenciales ordinarias basado en la cuantificacion de las variables de estado. Utilizando conceptos de integracion impl´ icita, el nuevo metodo denominado BQSS (Backward Quantized State Systems) permite integrar sistemas stiff de manera eficiente. Siendo el primer metodo de cuantificacion para sistemas stiff, BQSS es en s´ iu n

  13. Avaliação da resistência à corrosão de aços inoxidáveis com Nb endurecíveis por precipitação

    Microsoft Academic Search

    Luiz Carlos Casteletti; Frederico Augusto Pires Fernandes; Amadeu Lombardi-Neto; Carlos Alberto Picon; Germano Tremiliosi-Filho

    2010-01-01

    Precipitation hardening stainless steel was developed from 1945 in consequence of the aerospace industry needs. These steels possess substitutional martensitic microstructures that can be hardened by precipitation lately. On this work it was produced two steels with alternative compositions using Nb as a precipitate former, as well a steel PH13-8Mo for comparison purposes in terms of mechanical and corrosion resistance.

  14. Parcelas de agrado alrededor de Santiago y Valparaíso: ¿Migración por amenidad a la chilena?

    Microsoft Academic Search

    Rodrigo Hidalgo; Axel Borsdorf; Felipe Plaza

    2009-01-01

    The article analyzes the legal framework, distribution and spatial structure of amenity migration in mountain regions in Chile (Cordillera de la Costa, Cordi- llera de los Andes) and their trends in recent decades. Presents three case studies, two in mountainous regions and one in a semi-mountainous area. Ba- sed on qualitative data (interview with experts), and the relations between neig-

  15. Relatrio Tcnico Sinttico Estimativa das Emisses de CO2 por Desmatamento

    E-print Network

    carbono na vegetação e de emissões, o qual combina mapas anuais de novas áreas desmatadas gerados pelo conversão do carbono na biomassa em gases, assim como uma nova representação do processo de restabelecimento

  16. Qu es un ensayo clnico sobre la infeccin por el VIH/SIDA?

    E-print Network

    Levin, Judith G.

    efectos secundarios imprevisibles. · Es posible que los protocolos exijan al paciente bastante tiempo y frecuentes viajes al sitio del ensayo. Un servicio del Departamento de Salud y Servicios Humanos de los estudio? · ¿Cuánto tiempo durará el estudio? · ¿Quién me presta

  17. Sistema de información ferroviaria por teléfono: propuesta de una metodología de diseño de gestores de diálogo

    Microsoft Academic Search

    R. San-Segundo; J. M. Montero; J. Ferreiros; J. Macías-Guarasa; J. M. Pardo

    Resumen: En esta contribución se describen las fases llevadas a cabo en el desarrollo un servicio de información ferroviaria en castellano, presentándose una nueva metodología para el diseño de gestores de diálogo en servicios automáticos de información. En esta metodología se propone la combinación de diferentes fuentes de información: intuición, observación y simulación, para definir y evaluar alternativas de diseño.

  18. Gestión del riesgo de asma por haba de soja: monitorización y vigilancia

    Microsoft Academic Search

    Joan R. Villalbí; Dolors Carrascal; Joan A. Caylà; Pau Rodríguez; M. Cruz; Josep M. Pintó

    ObjectivesAllergen emissions during soybean unloading operations in the Barcelona harbor have caused asthma epidemics. The present article aimed to describe the surveillance and control measures carried out by the public health services.

  19. El estado del arte en las infecciones producidas por el virus del papiloma humano (VPH)

    Microsoft Academic Search

    R EINA; NUBIA M UÑOZ

    SUMMARY Anogenital human papillomavirus infection is the most frequent sexually transmitted disease. Around one hundred genotypes have been identified in humans, and 40 infect the genitalia and anal regions. Fifteen genotypes, classified as high- risk HPVs, are the necessary cause of cervical cancer and have been involved as carcinogenic agents for cancer of the vulva, vagina, anus, penis, and oropharyngeal

  20. Tumores en pacientes con infección por el virus de la inmunodeficiencia humana: estudio de 139 casos

    Microsoft Academic Search

    Germán Ramírez-Olivencia; María Eulalia Valencia-Ortega; Luz Martin-Carbonero; Victoria Moreno-Celda; Juan González-Lahoz

    2009-01-01

    Background and objectiveSince the introduction of highly active antiretroviral therapy (HAART), the natural history of HIV infection has been altered by an increasing survival. Following this, neoplastic diseases have become more common in HIV positive patients. The purpose of this study was to describe the types of tumor, clinical features and prognosis of HIV infected patients with malignant diseases.

  1. Influencia de politicas ambientales en la captura de carbono por parte de las masas forestales

    Microsoft Academic Search

    Luis Diaz Balteiro

    2002-01-01

    RESUMEN: Desde que recientemente se ha reparado en los ecosistemas forestales como posibles sumideros del CO2 atmosf�rico, se ha propuesto el establecimiento de masas forestales como medida para reducir las concentraciones de este gas. Esto llevar�a a que las plantaciones forestales presentaran objetivos adicionales de car�cter ambiental. Bajo la �ptica de un propietario privado o un posible inversor en esta

  2. Síndrome de violencia extrema por maltrato. Cuando el problema toca fondo

    Microsoft Academic Search

    Carlos Baeza Herrera; Héctor Sanjuán Fabián; Luis Manuel García Cabello; Alberto León

    Background. Battered child syndrome includes a wide spectrum. No organ or system of the body has been spared of physical damage caused by this social phenomenon. Material, method and results. The purpose of this report is to describe three of 54 patients, who suffered extreme violence by physical abuse. One of them sustained esophageal rupture and pulmonary contusion and had

  3. PREJUICIO Y DISTANCIA SOCIAL HACIA PERSONAS HOMOSEXUALES POR PARTE DE JÓVENES UNIVERSITARIOS

    PubMed Central

    Fernández Rodríguez, María del C.; Squiabro, José Calderón

    2014-01-01

    Se realizó un estudio descriptivo transversal con el propósito de explorar actitudes de rechazo y distancia social hacia las personas gays y lesbianas (GL) en 565 universitarios. Se utilizó una escala para medir Prejuicio y otra escala para medir Distancia Social. Los participantes reflejaron niveles moderados de prejuicio y distancia social (DS) hacia las personas gays y lesbianas. Los varones (M=104.5, DT= 27.47) mostraron significativamente más prejuicio que las mujeres (M=98.8, DT= 23.41). Los hombres (M=22.7, DT= 7.00) mostraron significativamente mayor DS que las mujeres (M=21.1, DT= 5.41). Las personas que asisten con regularidad a la iglesia mostraron más prejuicio y DS que los que no asisten. Se analiza importancia de incluir el tema de la diversidad sexual a través del currículo para desmontar prejuicios hacia la comunidad homosexual. PMID:25606066

  4. Distribución por diagnósticos de las cefaleas que acuden a los servicios de urgencias de atención primaria

    Microsoft Academic Search

    M. Ruiz; C. León; J. Castillo; M. Martínez; S. Sánchez; E. Quintela

    2010-01-01

    IntroductionEven though the existing literature shows that the tension headache is the most common in the general population, the greatest demand comes from the migraine. Many migraine suffers do not consider their headaches as a disease, so that their physician may not be aware of its diagnosis.

  5. Detección de infección por Coccidioides immitis en zonas del estado de Coahuila, México

    Microsoft Academic Search

    R. LÓPEZ-MARTÍNEZ

    SUMMARY Detection of Coccidioides immitis infection in Coahuila, Mexico. Coccidioidomycosis is a mycosis firstly pulmonar caused by Coccidioides immitis; it can be disseminated to central nervous system, bones and skin, principaly. In Mexico, the real frequency of the disease is unknown. The aim of this work was to determine, by skin test and by serology, the infection cases by C.

  6. O Uso da Medicina Alternativa e Complementar por Pacientes com Epilepsia: Risco ou Benefício?

    Microsoft Academic Search

    Auro Mauro Azevedo; Neide Barreira Alonso; Luís Otávio; Ferreira Caboclo

    The use of alternative and complementary medicine for patients with epilepsy: risk or benefit? Objective: This article evaluated the use of complementary and alternative medicine (CAM) in epileptic patients. Methods: Ninety seven patients answered a standardized questionnaire about CAM use. Results: Seventy-four patients (76%) reported having used at least one modality of MAC. The most common modalities reported by these

  7. Dos décadas de política de vivienda en Bogotá apostando por el mercado

    Microsoft Academic Search

    Nicolás Cuervo; Samuel Jaramillo

    2009-01-01

    La política de vivienda que se ha desarrollado en Colombia durante las dos últimas décadas ha tenido la orientación de claros principios liberales. A comienzos de los años noventa se introdujeron dos reformas substanciales al respecto: la eliminación de las particularidades de la línea de financiamiento de la rama, que fue homologada al resto del sector financiero, bajo el principio

  8. Un caso de pseudomiasis por larvas de Hermetia illucens (Diptera: Stratiomyidae) en un bovino

    Microsoft Academic Search

    Pablo Manrique-Saide; Roger I. Rodríguez-Vivas; Manuel Quiñones Rodríguez

    SUMMARY. A case of bovine pseudomyiasis caused by Her- metia illucens larvae (Diptera: Stratiomyidae). Introduction. Hermetia illucens is a dipterous which can produce gastroenteric pseudomyasis in humans and domestic animals. The larvae develop in a wide range of animal and vegetable decaying materials and can be accidentally ingested by

  9. Colonização do substrato capim-elefante suplementado com farelos por Pleurotus ostereatus

    Microsoft Academic Search

    Lorena Pastorini Donini; Eduardo Bernardi; José Soares

    Most of the edible fungi presents good indexes of development of the mycelium in different types of substrate, being, therefore of great importance the type of used substrate, propitiating the fast colonization and force of the mycelium. Thus, this research were carried out to study the growth rate of three strains of Pleurotus ostreatus in elephant grass supplemented with brains

  10. La marea roja causada por el dinoflagelado Alexandrium tamarense en la costa Pacífica colombiana (2001)

    Microsoft Academic Search

    Ingrid García-Hansen; Roberto Cortés-Altamirano; Arturo P. Sierra-Beltrán

    From April 26th to May 15th 2001, a large algae bloom was observed off Tumaco Bay on the Pacific coast of Colombia. This was the first harmful algae bloom (HAB) reported in the region, and reached Gorgona Island, about 120 km north. A year later, starting March 2002, an offshore HAB developed from Cabo Corrientes North to Solano Bay. The

  11. Association of the NADPH:protochlorophyllide oxidoreductase (POR) with isolated etioplast inner

    E-print Network

    Timko, Michael

    with a paracrystalline membrane structure, the PLB. Keywords: etioplasts, chloroplasts, NADPH the differentiation of proplastids or etio- plasts to chloroplasts. Etiolated angiosperms posses photoreceptors-photosynthetic state to a photosynthetically active chloroplast. The differentiation of etioplasts to chloro- plasts

  12. RELAÇÕES HÍDRICAS EM MUDAS DE Eucalyptus citriodora Hook., EM TUBETES, ACLIMATADAS POR TRATAMENTOS HÍDRICOS

    Microsoft Academic Search

    Antônio Cláudio Davide; Letícia Renata de Carvalho

    The water relations for E citriodora seedlings were determined, aclimatated for five treatments of irrigation. The seedlings were produced in plastic tubes wit h capacity of 50cm 3, filled with a mixture of 46% vermiculite (46%), carbonized rice shuch (46%) e subsoil (8%). The tests begun 70 days after seed. The experimental design was stablished as randomized block in four

  13. Ribosomal RNA-based analysis of the bacterial flora from the conjunctivae of cattle with bovine keratoconjunctivitis (BKC).

    PubMed

    Hare, William R; Hoyt, Phillip G; Hohn, Christina; Higgins, James A

    2008-10-15

    Bovine keratoconjunctivitis (BKC), colloquially referred to as 'pinkeye', is a disease affecting cattle worldwide; it costs cattle producers millions of dollars in economic loss annually. While Moraxella spp. are the primary etiologic agent of pinkeye, surveys of flora from the conjunctivae of livestock from around the world have indicated that a variety of bacterial commensals occupy this niche. We used molecular biology-based methods to determine the composition of bacterial flora in the conjunctivae of normal dairy and beef cattle from Maryland (n=113), and beef cattle with clinical BKC from Louisiana (n=42). Three regimens were used: 16S rRNA PCR and DGGE analysis of amplicons; 16S rRNA PCR and cloning of amplicons into Escherichia coli followed by screening and sequencing of clones harboring inserts; and culture of bacteria on chromogenic agar followed by 16S rRNA PCR and sequencing. Most taxa were comprised of saprophytes found in the environment, such as Bacillus, Pantoea, E. coli, and Exiguobacterium. Moraxella spp. were infrequently observed. Some species, such as Propionibacterium acnes, represent taxa not previously associated with the conjunctivae. Bacillus pumilus and Bacillus licheniformis isolates from the conjunctivae of Maryland cattle were genetically distinct from isolates previously implicated in septic infections in cattle at the same location. We conclude that employing 16S rRNA-based methods for bacterial identification can be useful in defining the flora present in the conjunctivae of normal cattle, and those with BKC. PMID:18513895

  14. Sequence divergence in two tandemly located pilin genes of Eikenella corrodens.

    PubMed Central

    Tønjum, T; Weir, S; Bøvre, K; Progulske-Fox, A; Marrs, C F

    1993-01-01

    Eikenella corrodens normally inhabits the human respiratory and gastrointestinal tracts but is frequently the cause of abscesses at various sites. Using the N-terminal portion of the Moraxella nonliquefaciens pilin gene as a hybridization probe, we cloned two tandemly located pilin genes of E. corrodens 31745, ecpC and ecpD, and expressed the two pilin genes separately in Escherichia coli. A comparison of the predicted amino acid sequences of E. corrodens 31745 EcpC and EcpD revealed considerable divergence between the sequences of these two pilins and even less similarity to EcpA and EcpB of E. corrodens type strain ATCC 23834. EcpC from E. corrodens 31745 displayed high degrees of homology to the pilins of Neisseria gonorrhoeae and Pseudomonas aeruginosa. EcpD from E. corrodens 31745 showed the highest homologies with the pilin of one of the three P. aeruginosa classes, whereas EcpA and EcpB of strain ATCC 23834 most closely resemble Moraxella bovis pilins. These findings raise interesting questions about potential genetic transfer between different bacterial species, as opposed to convergent evolution. Images PMID:8478080

  15. [Recurrent chancriform mucous membrane ulcer in plasmacytoma with secondary IgA deficiency. Pyoderma chancriforme of the tongue].

    PubMed

    Hegemann, B; Helmbold, P; Dickert, C; Marsch, W C

    2001-09-01

    A 66 year old patient presented with a nine month history of recurrent oral ulcerations involving the tongue. We diagnosed chancriform pyoderma and a previously not identified multiple myeloma with secondary immunoglobulin deficiency. Clinically and histologically we excluded a necrotizing ulcerative stomatitis as found in individuals with cellular immunodeficiency as in late-stage HIV-infection. On culture only Neisseria catarrhalis was found. Chancriform pyoderma is often associated with local bacterial infections, especially Staphylococcus aureus. The most common sites are the genitalia and periorbital region; involvement of the oral mucosa is uncommon. To the best of our knowledge, this is the fourth reported case with tongue lesions. The multiple myeloma-associated immunoglobulin deficiency might have facilitated the oral manifestations of chancriform pyoderma. PMID:11572076

  16. Comparative in-vitro activity of tigemonam, a new monobactam.

    PubMed

    Rylander, M; Gezelius, L; Norrby, S R

    1988-09-01

    The in-vitro activity against Gram-negative aerobic bacterial pathogens of a new oral monobactam, tigemonam, was compared with those of amoxycillin/clavulanic acid, aztreonam, cefaclor, ceftazidime, cefuroxime, ciprofloxacin, co-trimoxazole and gentamicin, by an agar-dilution method. Tigemonam inhibited 90% of strains tested of Escherichia coli, Klebsiella spp., Proteus spp., Salmonella spp., Haemophilus influenzae and Branhamella catarrhalis at concentrations of 0.25 mg/l or below. The MIC90 for Enterobacter spp. was 16 mg/l and for Citrobacter 4 mg/l. Pseudomonas aeruginosa was resistant to tigemonam but susceptible to aztreonam. In comparison with the other oral antibiotics tested, tigemonam was generally more active. Tigemonam showed minimal inoculum dependence, between 10(2) and 10(6) cfu per spot. It is concluded that tigemonam is a candidate for clinical trials in patients with infections caused by Gram-negative aerobes other than pseudomonas and acinetobacter. PMID:3182428

  17. Beta-lactamase-producing bacteria in head and neck infection.

    PubMed

    Brook, I

    1988-04-01

    We have summarized our experience in recovery of beta-lactamase-producing bacteria (BLPB) in head and neck infection (HNI). These HNI include conjunctivitis, serous and chronic otitis media, cholesteatoma, chronic mastoiditis, chronic sinusitis, adenoiditis, recurrent tonsillitis in children and adults, peritonsillar abscess, and retropharyngeal abscess. Beta-lactamase-producing bacteria were found in 262 (51%) of 513 patients with HNI; 72% had aerobic BLPB and 57% had anaerobic BLPB. The infections, where these organisms were most frequently recovered, were adenoiditis (85% of patients), tonsillitis in adults (82%) and children (74%), retropharyngeal abscess (71%), and chronic otitis media (57%). The predominant BLPB were Staphylococcus aureus (49% of patients with BLPB), the Bacteroides-melaninogenicus group (28%), the Bacteroides fragilis group (20%), Pseudomonas aeruginosa (13%), Hemophilus influenzae (5%), and Branhamella catarrhalis (3%). The high incidence of recovery of BLPB in head and neck infections may have important implications on the antimicrobial management of these infections. PMID:3258396

  18. Far from superficial: microbial diversity associated with the skin and mucus of fish

    USGS Publications Warehouse

    Cipriano, Rocco C.; Dove, Alistair

    2011-01-01

    During horizontal or water-borne infection involving an obligate pathogen (e.g. – Aeromonas salmonicida, cause of furunculosis), the pathogen interacted with and influenced the microbial diversity of the dermal mucus of fish. Prior to infection, the prevalent bacterial flora cultured from juvenile Atlantic salmon (Salmo salar) included Pseudomonas fluorescens, Comomonas terrigenia, Acinetobacter sp., Moraxella sp., Pseudomonas dimunita, Alcaligenes denitrificans, Pseudomonas pseudoalcaligenes, and Pseudomonas alcaligenes, Serratia liquefaciens, Aeromonas hydrophila, other motile Aeromonas spp., and Corynebacterium aquaticum. After A. salmonicida was initially detected in this population as an external mucus infection, Acinetobacter sp., Moraxella sp., C. terrigenia, P. fluorescens, and P. dimunita, Staphylococcus sp., and A. hydrophila, were also present in appreciable numbers. Within several weeks, however, the A. salmonicida infection amplified and composed 78% of the total flora in the mucus. Only P. dimunita (4%). P. fluorescens (2%), and C. terrigenia (1%) were cultured at that time and more than a third of these fish showed evidence of a systemic A. salmonicida infection within their kidneys. Eight weeks after oral oxytetracycline treatments, A. salmonicida was no longer isolated from the mucus or kidneys of any fish and glucose inert or other oxidative microbes (e.g., P. fluorescens, C. terrigenia, Acinetobacter sp., Moraxella sp.) were beginning to repopulate the external surface of the salmon in increasing frequency. Still present and composing fairly large percentages of the total flora were A. hydrophila, as well as Enterobacter sp., and P. putrefaciens. A normal microbial diversity was re-established as the fish recovered. In another investigation, reduced biological diversity was noted in the dermal mucus among smallmouth bass that were sampled from the Jackson River (Covington, VA). In these fish, A. hydrophila and P. putrefaciens were the two predominant microorganisms composing 49.5% and 31.2% of the total bacterial flora, despite the absence of systemic infection or any other clinical signs of disease. In another instance, P. fluorescens was the sole bacterium associated with the surface of Atlantic salmon eggs regardless of their viability at the eyed stage of development. Collectively, these results indicate that the kinetics and distributions of the surface bacterial flora on aquatic organisms is affected by numerous factors including pathogen invasion, environmental conditions, and fish culture practices.

  19. CTS NO ENSINO DE BIOLOGIA: UMA APLICAÇÃO POR MEIO DA ABORDAGEM DO COTIDIANO CTS EN LA ENSEÑANZA DE LA BIOLOGÍA: UNA APLICACIÓN POR MEDIO DEL ABORDAJE DE LO COTIDIANO

    Microsoft Academic Search

    Denise de Freitas; Marcos Lopes de Souza

    Resumo O objectivo desta investigação foi identificar como os professores explicitam e elaboram suas aulas tomando como perspectiva a integração dos conhecimentos escolares às ações quotidianas dos alunos. Foram realizadas entrevistas com professores do ensino médio de uma cidade do interior do Brasil e acompanhas algumas de suas aulas. Interpretando os resultados percebe-se a existência de diferentes compreensão e abordagens

  20. Análisis comparativo de las ecuaciones desarrolladas por Jackson et al y por el American College of Sports Medicine (ACSM) para predecir el consumo máximo de oxígeno en estudiantes de fisioterapia

    Microsoft Academic Search

    R. Ramírez Vélez; P. Delgado

    2008-01-01

    ObjectiveComparativily to analyze the equations developed by Jackson et al, in order to prediction of functional capacity aerobic exercise without making exercise (Nonexercise regression models to estimate peak oxygen consumption [PAR\\/PAF]) and the calculation made in endless band propose by ACSM (American College of Sports Medicine) in a group of students physical therapy.

  1. * El Seguro mvil de AT&T est garantizado por Continental Casualty Company, una compaa miembro de CNA y administrado por Asurion Protection Services, LLC, el representante autorizado de CNA para los clientes de AT&T.

    E-print Network

    Fisher, Kathleen

    prima incluye cargos de facturación pagaderos a AT&T y un cargo administrativo pagadero a Asurion. Aviso batería estándar, el cargador de batería estándar, el cable USB y la tarjeta SIM. #12;Proceso simple de

  2. Los alumnos/as podrn realizar la matrcula personalmente, por correo electrnico o por fax. La hoja de inscripcin tiene que ir acompaada del resguardo bancario del pago del curso en

    E-print Network

    Campanario, Juan Miguel

    curso en la que tiene que aparecer, imprescindiblemente, el código del curso, el nombre del alumno/a y su NIF. Si el curso tiene descuento para estudiantes universitarios, es obligatorio presentar una fotocopia de la matrícula universitaria del curso 2011-2012. La organización de los cursos no se hace

  3. QUANDO A HISTÓRIA INVADE A CENA: O CASO VLADIMIR HERZOG (RE)SIGNIFICADO POR FERNANDO PEIXOTO EM \\

    Microsoft Academic Search

    Ludmila Sá de Freitas

    Primeiramente, deve-se ressaltar que a proposta desta pesquisa (que se encontra em estágio inicial e que, portanto, muitos questionamentos são apenas apontados neste texto) é uma reflexão sobre a encenação do espetáculo teatral Ponto de Partida de Gianfrancesco Guarnieri, em 1976, no Teatro TAIB (SP), numa produção de Othon Bastos Produções Artísticas e Documenta Produções Artísticas. Neste sentido, tais análises

  4. EVALUACIÓN DE PÉRDIDA DE BIOMASA OCASIONADA POR MELANOPLUS LAKINUS (SCUDDER) (ORTHOPTERA: ACRIDIDAE) EN PASTIZALES DE DURANGO, MÉXICO

    Microsoft Academic Search

    J. NATIVIDAD GURROLA REYES; I. CHAÍREZ; M. MURILLO ORTÍZ

    The loss of biomass caused by the grasshopper Melanoplus lakinus (Scudder) was assessed in arbosufrutescent medium grassland composed of Chloris virgata Sw. and Rynchelytrum repens (Willd.) C.E. Hubb., associated with Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths and B. curtipendula (Michx.) Torr. The study area was Castillo Nájera, Durango, Mexico. Three densities of grasshopper (7, 14 and 28 insects\\/m

  5. Sistema de injeção em fluxo espectrofotométrico para monitorar peróxido de hidrogênio em processo de fotodegradação por reação foto-Fenton

    Microsoft Academic Search

    Mirela C. Oliveira; Raquel F. Pupo Nogueira; José A. Gomes Neto; Wilson F. Jardim; Jarbas J. R. Rohwedder

    2001-01-01

    Fenton processes (Fe2+\\/H2O2\\/UV). Sample is injected manually in a carrier stream and then receives by confluence a 0.1 mol L-1 NH4VO3 solution in 0.5 mol L -1 H 2SO4 medium. The product formed shows absorption at 446 nm which is recorded as a peak with height proportional to H 2O2 concentration. The performance of the proposed system was evaluated by

  6. Anemia por deficiencia de hierro en mujeres mexicanas en edad reproductiva: historia de un problema no resuelto

    Microsoft Academic Search

    Esther Casanueva; Luz María de Regil; María Fernanda Flores-Campuzano

    2006-01-01

    Objective. To describe the prevalence of iron deficiency anemia in the past 66 years among Mexican women on re- productive age, and to analyze the efficacy of interventions implemented for its prevention and control. Material and Methods. Observational and clinical epidemiological stud- ies as well as federal intervention programs published be- tween 1939 and 2005 having original data related to

  7. Produção de matéria seca e absorção de nutrientes pelo milho em razão da saturação por bases e da adubação potássica

    Microsoft Academic Search

    MARCELO ANDREOTTI; EUCLIDES CAXAMBU ALEXANDRINO DE SOUZA; CARLOS ALEXANDRE COSTA CRUSCIOL; JOÃO DOMINGOS RODRIGUES; LEONARDO THEODORO BÜLL

    2000-01-01

    The purpose of this research was to study dry matter yield and nutrients uptake by corn plants as a function of potassic fertilization and of soil basis saturation. An experiment was conducted under greenhouse conditions, using an early single hybrid corn Zêneca 8392 grown in 30 L pots, tested with three types of soil (Quartzpsamment and two alic Dark-Red Latosol

  8. C ALCULO Y DISE ~NO ASISTIDO POR ORDENADOR DE TOMAS DE TIERRA CON MODELOS DE SUELO NO UNIFORMES

    E-print Network

    Colominas, Ignasi

    and design of substation grounding requires computing the distribu- tion of potential on the earth surface computation of substa- tion grounding systems in nonuniform soils is presented in this paper. The formulation |for reasons of human security| and the equivalent resistance of the earthing system |for reasons

  9. Casuística de acceso a la actualización de la prestación por desempleo: bases para un modelo de decisión

    Microsoft Academic Search

    Sonia Martín López; Gustavo Lejarriaga Pérez de las Vacas; Javier Iturrioz del Campo

    2005-01-01

    Since 1985, when Royal Decree 1044\\/85, dated 19 June 1985, provided for the receipt of unemployment benefit in a lump sum payment, this instrument has enabled the creation of several professional and business initiatives that have undoubtedly contributed to the generation of stable employment. There have been numerous stages in the development of this tool to date. In each of

  10. Highlights 118 En este nmero iniciamos la edicin de Highlights 2010, por lo que queremos aprovechar la

    E-print Network

    of RuxWySez Catalyst as a Cathode Electrode in a Polymer Electrolyte Membrane Fuel Cell. Fuel Cells Fuel Cell Fed with Spend Organic Extracts from Hydrogenic Fermentation of Organic Solid Wastes. J. New cathode catalyst for PEM fuel cells. J. Power Sources, In press 2010. - H.M. Poggi-Varaldo, A. Carmona

  11. CARACTERIZACIÓN DE SÓLIDOS DE RIPIOS PETROLIZADOS POR MICROSCOPÍA DE BARRIDO ELECTRÓNICO: EFECTO EN LA DESHIDRATACIÓN DEL PETRÓLEO

    Microsoft Academic Search

    Gerson Chávez Narváez; Nolberto Piña; Gladys Bravo; Cesar Barreto; Luis Martinez; Gerardo Osorio; Freddy Ysambet; William Campo; Víctor Morón; Ronald Reyes

    The stabilization of emulsions for crude oil rubbles solids is a problem for the petroleum industry due to the costs that it carries the biggest time in the dehydration treatment and therefore in its commercialization. In this work a study of the morphology and chemical composition of the crude oil rubbles solids for scanning electronic microscopy (SEM) and their influence

  12. Validación de la transmisión por correo electrónico de la retinografía de pacientes diabéticos para detectar retinopatía en atención primaria

    Microsoft Academic Search

    Eduardo Molina Fernández; M. Sacramento Valero Moll; Miguel Pedregal González; José Calvo Lozano; José Luis Sánchez Ramos; Eusebio Díaz Rodríguez; Rosario Uceda Torres

    2008-01-01

    ObjectiveEvaluate concordance in biomicroscopy evaluation of diabetic retinopathy degree among ophthalmologists. Validation of e-mail transmission of digital fundus photographs of type 2 diabetes patients as a method of diabetic retinopathy detection.

  13. Diferencias por sexo en los criterios y estándares de productividad científica y docente en profesores funcionarios en España

    Microsoft Academic Search

    Juan Carlos Sierra; Gualberto Buela-Casal; María Paz Bermúdez; Pablo Santos-Iglesias

    2009-01-01

    El objetivo del presente trabajo es analizar las diferencias en la opinión que tienen los profesores y pro- fesoras universitarios españoles sobre los criterios y estándares de productividad científica, así como el número mínimo en éstos para ser acreditado como Profesor Titular de Universidad (PTU) o como Catedrático de Universidad (CU). Para ello se encuestó a una muestra de 960

  14. Efectos de Campos Magnéticos en las Tasas de Consumo de Madera por Coptotermes formosanus, la Termita Subterránea de Formosa.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sixty groups of 500 workers and 50 soldiers of Coptotermes formosanus were maintained in costume designed containers and fed with a piece of red oak wood (Quercus rubra). Twenty of these groups were exposed to permanent magnets with a flux of 800 G. Another 20 groups were exposed to a permanent mag...

  15. La Accin Innovative Training Networks (ITN) tiene como objetivo formar, por medio de una red internacional de centros pblicos y

    E-print Network

    Escolano, Francisco

    . 10:00 Acciones Marie Sklodowska Curie: Convocatorias 2015 Innovative Training Networks (ITN´Enginyeria Química de la Universitat de València. Evaluadora. 13:15 Otras Acciones Marie Sklodowska Curie: próximas - Nivell 3 Avda. Blasco Ibañez, 13 Valencia Horizonte2020 INNOVATIVE TRAINING NETWORKS Marie Sklodowska

  16. Guaraná (Paullinia cupana) para fadiga induzida por quimioterapia antineoplásica Guarana (Paullinia cupana) for chemotherapy-related fatigue

    Microsoft Academic Search

    Vanessa da Costa Miranda; Damila Cristina Trufelli; Ângelo Bezerra de Souza Fêde; Flávia Delgado Martins; Lucas Scatigno Saad; Vinicius de Oliveira; Rachel Riechelmann; Auro Del Giglio

    Objective: To evaluate the effect of guarana in patients with solid tumors starting on chemotherapy, insofar as chemotherapy-induced fatigue and depressive symptoms are concerned. Methods: This is a double-blind, cross-over trial in which chemotherapy naive patients were randomized to take either guarana 75 mg or placebo tablets during the first cycle of chemotherapy and then switched to the alternative for

  17. Guarana (Paullinia cupana) for chemotherapy-related fatigue Guaraná (Paullinia cupana) para fadiga induzida por quimioterapia antineoplásica

    Microsoft Academic Search

    Vanessa da Costa Miranda; Damila Cristina Trufelli; Ângelo Bezerra de Souza Fêde; Flávia Delgado Martins; Lucas Scatigno Saad; Vinicius de Oliveira; Rachel Riechelmann; Auro Del Giglio

    Objective: To evaluate the effect of guarana in patients with solid tumors starting on chemotherapy, insofar as chemotherapy-induced fatigue and depressive symptoms are concerned. Methods: This is a double-blind, cross-over trial in which chemotherapy naive patients were randomized to take either guarana 75 mg or placebo tablets during the first cycle of chemotherapy and then switched to the alternative for

  18. Fecha de publicacin: 31/07/2013 RESOLUCIN POR LA QUE SE PUBLICA LA LISTA DEFINITIVA DE

    E-print Network

    Escolano, Francisco

    DEL "PROGRAMA IMPULSO: III CONVOCATORIA DE PREMIOS A IDEAS Y PROYECTOS DE EMPRESAS DE BASE TECNOLÓGICA producción masiva de insectos beneficiosos para el control biológico de plagas FIRMA Mª Ángeles Marcos García hongos y levaduras FIRMA Federico López Moya TITULO Aplicación de un agente de control biológico en

  19. PUBLICACIONES INGRESADAS EN EL CENTRO DE DOCUMENTACIN ESTADSTICA ltimas adquisiciones del Centro de Documentacin Estadstica, por compra y donacin

    E-print Network

    Autonoma de Madrid, Universidad

    Madrid: Unión Fenosa CDE/DES.CD.323 Informe de responsabilidad social corporativa... / Banco Popular Español [Madrid]: Banco Popular Español, 200?- CDE/AR.54 Mapa empresarial de Madrid Norte: Proyección

  20. Recubrimientos de TiAlN sobre acero ASTM A36 por el proceso de sputtering reactivo RF

    Microsoft Academic Search

    Franz Quesada; Álvaro Mariño

    TiAlN films were deposited on ASTM A36 steel by RF reactive sputtering process using a TiAl (60\\/40%) target. Films were sputtered at two different relative pres- sures, 0,05 and 0,1, keeping constant the Argon pressure. The films were deposi- ted on samples with different pretreatments: as received material, hardened and quenched, plasma-nitrided, and with initial coatings of Ti and TiN.

  1. ¿Por que los niños abandonan la escuela? Determinantes de la desercion estudiantil en los colegios oficiales de Cartagena de Indias

    Microsoft Academic Search

    Jorge Alvis; William Arellano

    2009-01-01

    El estudio analiza los factores asociados a la desercion escolar en el distrito de Cartagena y dos estudios de caso correspondientes a las instituciones educativas San Felipe Neri y Tecnica Agropecuaria La Boquilla. Se incluyen variables de análisis relacionadas con las caracteristicas individuales, socioeconomicas, academicas e institucionales de los estudiantes matriculados en el sector oficial como determinantes de esta situación.

  2. Efeito da idade cronológica na distribuição dos futebolistas por escalões de formação e pelas diferentes posições especificas

    Microsoft Academic Search

    H. A. Folgado; P. F. Caixinha; J. Sampaio; V. Maçãs

    Relative age effects among youth soccer players according to playing positions This study analysed the distribution of the birthdates from soccer players according to each player's age-group and specific position on the field. The players from the Lisbon Soccer Association (n=188) were divided into their age-groups during the 2004\\/2005 season: Under 8s (n=26), Under 11s (n=39), Under 13s (n=43), Under

  3. Disponibilidade de nutrientes na fitomassa produzida por cultivares de milho (Zea mays L.) de diferentes niveis tecnologicos

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Macronutrient uptake, accumulation, and exportation can be affected by corn genetic selection. The objectives of this study were to quantify macronutrient extraction and to evaluate C/P and C/N ratio. The experiment was established at Rolândia County, Paraná State. Ten corn varieties divided into pa...

  4. USO Y SELECCIÓN DE EMBALSES POR EL PATO MEXICANO (ANAS DIAZI) EN LA REGIÓN DEL LLANO, AGUASCALIENTES-JALISCO, MÉXICO

    Microsoft Academic Search

    Salvador Martín; MEDINA TORRES; Marcelo MÁRQUEZ OLIVAS; Edmundo GARCÍA MOYA

    The use of dams by the Mexican duck (Anas diazi Ridway, 1886) was documented between September 1994 and March 1995, in the region of El Llano, located in Mexico between Aguascalientes and Jalisco. The objective of this study was to determine which type of dam is more frequently used by this species, based on a use-availability water analyses. The results

  5. Meningites de repetição por malformação de base de crânio com fístula Repeated meningitis due to skull base malformation with fistula

    Microsoft Academic Search

    Wanderley Wesley Bonafé; Laura Mariana Martins; Ricardo Camillo de Almeida; Marisa Dolhnikoff

    RESUMO Objetivo: Relatar caso de meningite de repetição secun- dária à malformação da base do crânio, com fístula liquórica para a cavidade nasal. Descrição: Criança de 30 meses de idade chegou ao pron- to-socorro em mau estado geral, com quadro de pneumonia aguda, desidratação, marasmo e aumento do perímetro cefálico. Apresentava antecedente de meningites de repetição, sendo um episódio aos

  6. Programas Internacionales: distribucin por Centros del n de estudiantes que participan en Programas de Movilidad EXTERNOS ( * ) PROPIOS ( ** )

    E-print Network

    Autonoma de Madrid, Universidad

    ENFERMERÍA DE LA PAZ 0 0 1 1 1 1 E.U. DE ENFERMERÍA PUERTA DE HIERRO 1 1 3 3 4 4 E.U. DE FISIOTERAPIA DE LA.U. DE ENFERMERÍA PUERTA DE HIERRO 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 E.U. DE FISIOTERAPIA DE LA ONCE. 0

  7. Intervención arqueológica en la manzana formada por las calles Sant Antoni, del Carme, Sant Blai y Pintor Casanova

    Microsoft Academic Search

    Eduardo López Seguí; Laura Guillem Fernández; Virginia Barciela González

    2009-01-01

    Es presenten els resultats d’una intervenció arqueològica desenvolupada al nucli històric d’Alcoi, en compliment de la normativa que obliga a realitzar\\u000aactuacions arqueològiques prèvies a la construcció de noves edificacions en les àrees considerades “de protecció arqueològica”. El resultat d’aquesta és\\u000ala troballa de les restes de 15 dels habitatges que formaven part d’una illa de cases. No s’hi han

  8. Significados dos conceitos da termodinâmica e possíveis indicadores de invariantes operatórios apresentados por estudantes do ensino médio e técnico

    Microsoft Academic Search

    Edi Terezinha; Marco Antonio Moreira

    This study aimed at finding out the level of conceptualization assigned by students to basic concepts of thermodynamics (temperature, heat, internal energy, work and entropy) and inferring possible indicators of operational invariants. The research was carried out through a questionnaire with 99 students from Fundação Escola Técnica Liberato Salzano Vieira da Cunha (a technical high school) in Novo Hamburgo, Brazil.

  9. REMOCIÓN DE SÓLIDOS TOTALES DE VINAZAS POR ELECTROCOAGULACIÓN - ELECTROFLOTACIÓN TOTAL SOLID REMOVAL OF VINASSE BY ELECTROCOAGULATION - ELECTROFLOTATION

    Microsoft Academic Search

    JAVIER DÁVILA RINCÓN; NILSON MARRIAGA CABRALES; FIDERMAN MACHUCA MARTINEZ

    2009-01-01

    This article presents the results obtained in the treatment of vinasse from an alcohol distillery through a process of electrocoagulation - electroflotatión. Operating variables such as initial pH of the solution, current density and concentration of NaCl, as supporting electrolyte, were evaluated in a 2 3 factorial design using aluminum electrodes in a monopolar arrangement. The results show that this

  10. Análise de ácidos graxos não-esterificados de plasma humano por cromatografia gasosa capilar com injeção sem divisão de fluxo

    Microsoft Academic Search

    Jacqueline G. Ney; Alexandre G. Torres; Nádia M. F. Trugo

    2004-01-01

    ANALYSIS OF NON-ESTERIFIED FATTY ACIDS IN HUMAN PLASMA BY CAPILLARY GAS-CHROMATOGRAPHY WITH SPLITLESS INJECTION. The aim of the present work was to test the combination of non-esterified fatty acid (NEFA) isolation using fumed silicon dioxide with capillary gas-chromatography (C-GC) with splitless injection for the analysis of NEFAs in human plasma. Injection volume, solvent re-condensation and split purge flow-rate were the

  11. Uso de esteróides anabólicos androgênicos por praticantes de musculação de grandes academias da cidade de São Paulo

    Microsoft Academic Search

    Luciana Silvia Maria Franco Silva; Regina Lúcia de Moraes Moreau

    2003-01-01

    O objetivo desta pesquisa foi estimar o consumo e traçar o perfil dos usuários de esteróides anabólicos androgênicos (EAA) entre praticantes de musculação em três grandes academias de ginástica na cidade de São Paulo. Foi utilizado um questionário estruturado para ser respondido voluntária e anonimamente, com garantia explícita de confidencialidade para os mesmos. Os questionários ficaram disponíveis em três academias

  12. Diferimiento de Ingreso. Carreras Largas y Cortas. Proceso de admisin 2012 correspondiente a los asignados por el mecanismo OPSU

    E-print Network

    Vásquez, Carlos

    Diferimiento de Ingreso. Carreras Largas y Cortas. Proceso de admisión 2012 correspondiente a los: Edificio Administrativo, Planta Baja. b)Banco Mercantil, a nombre de: USB Tesorería, cuenta corriente nro inscribirán en la Universidad en el proceso 2012. 8. Los aspirantes a quienes se le otorgue el diferimiento

  13. Listado de cursos de verano por fecha de realizacin SEMANA DEL 30 DE JUNIO AL 6 DE JULIO

    E-print Network

    Escolano, Francisco

    PERSONAL: INTELIGENCIA EMOCIONAL Y LA PSICOLOGÍA POSITIVA EXCAVANDO PAPELES: LOS VALORES DEL PATRIMONIO JORNADA DE INNOVACIÓN EDUCATIVA: LAS INTELIGENCIAS MÚLTIPLES. DE LA TEORÍA A LA PRÁCTICA INTEGRACIÓN COMPETENCIAS PROFESIONALES: BIENESTAR EMOCIONAL, COACHING, CORPORALIDAD, COMUNICACIÓN Y LIDERAZGO LA MARINA

  14. Evaluación del sistema de información de Navarra sobre diagnósticos de infección por el VIH, 1985-2003

    Microsoft Academic Search

    Ana María Caro-Murillo; Concepción Moreno-Iribas; Fátima Irisarri; Pablo Aldaz; Víctor Napal; Carmen Varela Santos; Jesús Castilla

    2007-01-01

    Evaluation of the surveillance system for Human Immunodeficiency Virus Infections of Navarra, Spain, 1985-2003 Background: Navarra has an information system of HIV diagnoses working since the beginning of the epidemic up to the present day. This study aims at describing and evaluating this system, focusing on its sensitivity.

  15. Validación del termociclador TEMPER en ensayos basados en la amplificación de ácidos nucleicos por reacción en cadena de la polimerasa

    Microsoft Academic Search

    Anny Armas; Iria García; Enny Morales; Vivian Alonso; Idania González; Jorge G Martínez-Casado; Yenitse Perea; Yaimé González

    Validation of the TEMPER thermocycler in nucleic acid amplification by polymerase chain reaction. The thermocycler is a programmable cycling incubator that automatically performs the cyclic changes in temperature required for Polymerase Chain Reaction (PCR). In this paper we present the results of the Operational Qualification (OQ) of TEMPER thermocyclers (Center for Immunoassays, CIE). OQ is carried out to ensure that

  16. Frecuencia de las co-morbilidades psiquiátricas del eje i en los pacientes con diagnóstico de trastorno por estrés postraumático

    Microsoft Academic Search

    Adriana P. Bohórquez

    2009-01-01

    The clinical presentation of the psychiatric co-morbidities asso- ciated to the Post-traumatic Stress Disorder (TEPT) is described by means of a descriptive cross section observational study made in patients with a diagnosis of TEPT with antecedent of exhibition to combats, with military rank of professional, ser - geant major or official, hospitalized by the Service of Psychiatry of the Central

  17. Interact: um modelo de interao para interfaces 2D por manipulao direta MARCELO MEDEIROS CARNEIRO 1,2

    E-print Network

    @iprj.uerj.br 3 PETROBRAS ­ Petróleo Brasileiro S.A. CENPES ­ Centro de Pesquisas e Desenvolvimento Leopoldo A. Miguez de Mello Cidade Universitária, Quadra 7, Sala 9020, 21949­900 Rio de Janeiro, RJ, Brasil enio@cenpes.petrobras

  18. Análisis de la Amplitud de las Señales de Emisión Acústica Generadas por Corrosión Bajo Tensión en Latón-?

    Microsoft Academic Search

    P. A. Lapitz; J. Ruzzante; M. G. Alvarez; Agencia de Promoción Científica; Tecnológica Argentina

    2005-01-01

    The present work was aimed to characterize the acoustic emission (AE) response obtained during the propagation of stress corrosion cracks. The differences between the AE signals generated by transgranular and intergranular SCC were analyzed. For that purpose, slow strain rate tensile tests (SSRT) under potentiostatic control were performed. The systems studied were: 1) yellow brass in a 1M NaNO2 solution;

  19. Colloque international Por que facer Lingstica Romnica no sculo XXI ? (Saint-Jacques-de-Compostelle, 13/14 novembre 2008)

    E-print Network

    Paris-Sud XI, Université de

    d'Innsbruck), Johannes KRAMER (Université de Trèves), Stella MEDORI (Université de Corse), Jan CORGNALIOTTI (Université de Turin), Max PFISTER (Université de la Sarre, Sarrebruck) Galloromania : Jean-Paul) · */'kad-e/ (REW3 s.v. cadre/*cadre ; Éva BUCHI) · */'karpn-u/ (REW3 s.v. carpnus ; Stella MEDORI) Articles

  20. Caracterização espectral de Brachiaria brizantha cultivada em solo contaminado por hidrocarbonetos como instrumento de detecção de vazamentos em dutos

    Microsoft Academic Search

    Giuliana Clarice Mercuri Quitério

    This work aims to develop a hydrocarbon (HC) pipeline monitoring technique that is efficient, practical, economical and capable to detect small leakages precociously, which are barely discernible by current practices, and before they impact the environment evidently. The proposed method comprises the indication of a plant susceptible of being monitored through remote sensing and that responds to HCs leaks with

  1. Results of mujeres felices por ser saludables: a dietary\\/breast heafth randomized chnicail trial for latino women

    Microsoft Academic Search

    Marian L. Fitzgthhon; Susan M. Gapstur; Sara J. Knight

    2004-01-01

    Background: Data are limited on the efficacy of health-fo-cused interventions for young, low-acculturated Latino women. Because breast\\u000a cancer is the most commonly diagnosed cancer and the most common cause of cancer mortality in this population, combined interventions\\u000a that address both early detection and dietary patterns could help reduce both morbidity and mortality associated with breast\\u000a cancer in this underserved population.Purpose:

  2. Concentração de potássio na solução nutritiva e a qualidade e número de frutos de melão por planta em hidroponia

    Microsoft Academic Search

    Caciana Cavalcanti Costa; Arthur Bernardes Cecílio Filho; Rodrigo Luiz Cavarianni; José Carlos Barbosa

    2004-01-01

    The experiment was carried out in Jaboticabal-SP. The hybrid Bonus n 0 2 was cropped in randomized blocks design, in split-plot scheme and six replications. Fruit number per plant (2, 3, 4 e free fruit set) and potassium concentration in the nutrient solution (66, 115.5, 165 and 247.5mg L -1) were the studied factors. The increase of potassium concentration in

  3. Evaluación de un programa de monitoría de la calidad de los servicios otorgados por una Organización no Gubernamental

    Microsoft Academic Search

    Alma Lucila Sauceda-Valenzuela; Luis Durán-Arenas; Bernardo Hernández

    2000-01-01

    Objective. To evaluate the effect of a quality-monitoring program on the occurrence of sentinel events and on attitu- des and behaviors of personnel's responses in a Non-Gov- ernmental-Organization (NGO). Material and methods. Between 1998 and 1999, a quasi-experimental design of the before-after type was conducted in 13 NGO clinics. Analy- sis of changes in attitudes and behaviors consisted in differ-

  4. RACIONALIDADE AMBIENTAL POR PARTE DE PRODUTORES RURAIS SITUADOS NA REGIÃO DA NASCENTE DO RIO GRANDE

    Microsoft Academic Search

    Anna Carolina Salgado Jardim; Robson Amancio; Marcos Affonso Ortiz Gomes

    2006-01-01

    The objective of this article is to identify and characterize the environmental reasoning of local farmers and agriculturalists by understanding the world views, perceptions, knowledge bases, and socio-environmental practices of two rural communities situated in the proximities of the source of the River Rio Grande, in the municipality of Bocaina de Minas, Minas Gerais. Specifically, this article attempts to characterize

  5. Uso da segmentação por crescimento de regiões em imagem Ikonos na discriminação de tipologias da Floresta Ombrófila Mista

    Microsoft Academic Search

    Fernando Luís Dlugosz

    This paper describes the use of the segmentation technique by growing regions, available in software SPRING, to discriminate forest types in high resolution imagery. Digital image processing techniques are part of a major project which aims the environmental zoning of the Embrapa\\/Epagri Forest Reserve located in Caçador, southwestern Santa Catarina State, Brazil, including a management plan and the monitoring of

  6. La infección por el virus del papiloma humano, un posible marcador biológico de comportamiento sexual en estudiantes universitarios

    Microsoft Academic Search

    Miguel A Sánchez-Alemán; Felipe Uribe-Salas; Carlos J Conde-González

    2002-01-01

    Objective. To estimate the prevalence of Human papillomavirus (HPV) among university students and to use it as a biological marker to assess sexual behavior. Material and Methods. A cross-sectional study was carried out between 2000 and 2001 among 194 students at Universidad Autónoma del Estado de Morelos, Mexico. A data collection instrument was applied and genital samples were taken to

  7. Laboratorio 1: Analisis de datos univariantes Se toman, por ejemplo, los datos del fichero Nations.txt.

    E-print Network

    Gil, Ana Arribas

    programa (>): # Lectura de datos Datos table("C:/R-2.8.1/library/Rcmdr/etc/Nations.txt", header() barplot(table(Datos$region), xlab="region", ylab="Frecuencias") # Diagrama de tarta X11() pie(table(Datos$region), labels=levels(Datos$region), main="Distribucion de region", col=rainbow(length(levels(Datos$region)))) 4

  8. Análisis de las tendencias de mortalidad por cáncer de mama en Colombia y Bogotá 1981-2000

    Microsoft Academic Search

    Gustavo Hernández; Santiago Herrán; Luis Fernando Cantor

    Abstract Introduction: In Colombia, breast cancer mortality has increased. Paradoxically, Bogota with the highest rates showed a decreasing trend. In this study we examined age, cohort and period effects over breast cancer mortality trends in Bogotá and Colombia as a whole (excluding Bogota) between,1981 and 2000.

  9. EL GUSTO POR EL AUTO CUIDADO Y LA BÚSQUEDA DE ESTÍMULOS, MOTIVACIÓN Y PLACER EN EL EJERCICIO PROFESIONAL

    Microsoft Academic Search

    Santa Lázaro Fernández

    Al principio…. me iba a casa desanimada, pensando que era todo muy injusto. Me enfadaba. Ahora, tiempo después, no es que no piense lo mismo, pero ya lo tomo de otra manera, ya vi y comprobé, que los problemas e injusticias del mundo, no son culpa mía, que yo hago lo poco, que es muy poco, que puedo hacer para

  10. Efecto de la contaminación ambiental sobre las consultas por infecciones respiratorias en niños de la Ciudad de México

    Microsoft Academic Search

    MARTHA MARÍA TÉLLES-ROJO; ISABELLE ROMIEU; MARCO POLO-PEÑA; SILVIA RUIZ-VELASCO; FERNANDO MENESES-GONZÁLEZ; MAURICIO HERNÁNDEZ-AVILA

    1997-01-01

    Objective. To model the variability of medical visits by chil- dren for respiratory reasons as a consequence of the daily changes in environmental pollution observed in the emer- gency and family medicine departments of a hospital of the Instituto Mexicano del Seguro Social located in the south- west of Mexico City during 1993. Materials and meth- ods. The correlation between

  11. Avaliação da atividade genotóxica da finasterida, por meio do teste da mutação e recombinação somática, em asas de Drosophila melanogaster

    Microsoft Academic Search

    Antônio Joaquim de S. Castro; Júlio César Nepomuceno

    Finasteride is a synthetic drug that has been used in the prevention and treatment of prostate cancer, and also by people who have genetic predisposition to baldness. Its action is associated to the inhibition of 5-alpha reductase, enzyme that converts testosterone in dihydrotestosterone. Dihydrotestosterone is an androgynous responsible for the prostate growth, when it can be found in the gland,

  12. Enumeration and Characterization of Bacterial Colonists of a Submersed Aquatic Plant, Eurasian Watermilfoil (Myriophyllum spicatum L.).

    PubMed

    Chand, T; Harris, R F; Andrews, J H

    1992-10-01

    A simple procedure for enumerating and grouping the bacterial colonists of Eurasian watermilfoil (Myriophyllum spicatum L.) is described. Colony characteristics of bacteria associated with M. spicatum were better defined and more stable on nutrient-poor, diluted nutrient broth agar than on high-nutrient media. Acinetobacter, Cytophaga, Flavobacterium, Moraxella, Pseudomonas and/or Alcaligenes, and Vibrio/Aeromonas spp., as well as two highly fastidious unidentified bacterial groups (gram-negative rods and gram-negative cocci), were associated with cultured watermilfoil during January, February, May, June, July, and August 1988. In Lake Wingra (Madison, Wis.), Micrococcus spp. and enterobacters were also associated with Eurasian watermilfoil during July, August, and October 1987. PMID:16348792

  13. Microbial succession of grass carp (Ctenopharyngodon idellus) filets during storage at 4°C and its contribution to biogenic amines' formation.

    PubMed

    Wang, Hang; Luo, Yongkang; Huang, Heping; Xu, Qian

    2014-11-01

    Investigation on the microbial succession of grass carp filets during storage at 4°C was carried out. For identification, 16S rRNA genes of the isolated pure strains were sequenced and analyzed. Acinetobacter was dominant in fresh grass carp. Species from the genera Brevundimonas, Empedobacter, Pseudomonas, Microbacterium, Flavobacterium, Moraxella, Shewanella and Soonwooa were also detected at the initial day. The communities were dominated by Aeromonas and Acinetobacter after 6days. Aeromonas followed by Pseudomonas was the predominant genera at the end of shelf-life of grass carp, while other genera such as Shewanella, Acinetobacter, Flavobacteriaceae and Psychrobacter were present in smaller numbers. We investigated biogenic amines' (BAs) production by six strains isolated from spoiled grass carp filets. Shewanella putrefaciens showed significantly higher abilities to produce putrescine, than those from other genera. Aeromonas veronii revealed a strong ability to produce putrescine and cadaverine. However, Pseudomonas and Acinetobacter showed little ability to produce BAs. PMID:25194259

  14. AtfA, a new factor in global regulation of transcription in Acinetobacter spp.

    PubMed

    Withers, Ryan; Doherty, Geoff P; Jordan, Matthew; Yang, Xiao; Dixon, Nicholas E; Lewis, Peter J

    2014-09-01

    Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ? proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyi?ADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered. PMID:25047957

  15. Amino acid conjugated sophorolipids: A new family of biologically active functionalized glycolipids.

    PubMed

    Azim, Abul; Shah, Vishal; Doncel, Gustavo F; Peterson, Nicholas; Gao, Wei; Gross, Richard

    2006-01-01

    Sophorolipids (SLs) are extra cellular glycolipids produced by Candida bombicola ATCC 22214 when grown in the presence of glucose and fatty acids. These compounds have a disaccharide head group connected to a long-chain hydroxyl-fatty acid by a glycosidic bond. To explore structure-activity of modified SLs, a new family of amino acid-SL derivatives was prepared. Synthesized analogs consist of amino acids linked by amide bonds formed between their alpha-amino moiety and the carboxyl group of ring-opened SL fatty acids. Their preparation involved the following: (i) hydrolysis of a natural SL mixture with aqueous alkali to give SL free acids, (ii) coupling of free acids to protected amino acids using dicarbodiimide, and (iii) removing amino acid carboxyl protecting groups. These conjugates were evaluated for their antibacterial, anti-HIV, and spermicidal activity. All tested analogs showed antibacterial activity against both gram +ve and gram -ve organisms. Leucine-conjugated SL was most efficient. For example, the minimum inhibitory concentrations (MIC) for Moraxella sp. and E. coli were 0.83 and 1.67 mg/mL, respectively. Among the alkyl esters of amino acid conjugated SLs, the ethyl ester of leucine-SLs was most active. Against Moraxella sp., S. sanguinis, and M. imperiale, MIC values are 7.62 x 10(-4), 2.28 x 10-(3) and 1.67 mg/mL, respectively. All compounds displayed virus-inactivating activity with 50% effective concentrations (EC50) below 200 microg/mL. The EC50 of leucine-SL ethyl ester was 24.1 microg/mL, showing that it is more potent than commercial spermicide nonoxynol-9 (EC50 approximately 65 microg/mL). PMID:17105232

  16. ALTA DENSIDAD DE SIEMBRA EN LA PRODUCCIÓN DE MAÍZ CON IRRIGACIÓN POR GOTEO SUBSUPERFICIAL PRODUCTION OF MAIZE USING HIGH DENSITY SOWING AND SUBSURFACE DRIP IRRIGATION

    Microsoft Academic Search

    Aurelio Guevara-Escobar; Gabriela Barcenas-Huante; Francisco R. Salazar-Martínez; Enrique González-Sosa; Humberto Suzán-Azpiri; Monsanto México

    Maize is the main staple food in the Mexican diet and is also the most important irrigated crop. However, water shortage is very serious in México, and food security cannot be maintained without irrigation. To address this problem, the effect of maize density sowing was evaluated using a subsurface drip irrigation system. There were no differences in forage and grain

  17. Cambios del EEG por habituación y condicionamiento en niños de tres a 15 años que acuden al Instituto Nacional de Rehabilitación (INR)

    Microsoft Academic Search

    Héctor Brust-Carmona; Francisco Ramírez-Aboytes; José Martínez; Ángel Rodríguez Miguel; Blanca Flores-Ávalos; Oscar Yáñez-Suárez

    2009-01-01

    SUMMARY During waking, stimuli activate the sensory pathways giving rise to sensation and the response to such stimulation. The electrobiochemical changes and modifications in EEG rhythms event- related synchronization or event-related desynchronization, ERD or ERS) propagated in the specific and unspecific cortex are added to the changes elicited by the responses and to the new signals originated by those same

  18. ESTIMATIVAS DA AREA DE ATRAC~AO DE SISTEMAS N~AO LINEARES COM INCERTEZAS VARIANTES NO TEMPO POR MEIO DE UMA CLASSE DE

    E-print Network

    Pota, Himanshu Roy

    on the mean value theorem in conjunction to an approximation technique of convex sets to obtain this LDI differential inclusions, linear matrix inequalities Resumo-- Este artigo prop~oe utilizar uma classe particular forma de inclus~oes diferenciais lineares ou LDIs (do ingl^es, linear differential in- clusions), sendo

  19. Independencia para actividades de la vida diaria en ancianos previamente autónomos intervenidos por fractura de cadera secundaria a una caída tras 6 meses de evolución

    Microsoft Academic Search

    Eduardo Candel-Parra; María Pilar Córcoles-Jiménez; María Ángeles del Egidofernández; Ascensión Villada-Munera; María Delirio Jiménez-Sánchez; Mónica Moreno-Moreno; Miriam Carrión-González; Ángeles Denia-Cortés

    2008-01-01

    ObjectiveTo measure recovery of independence in activities of daily living (ADL) 6 months after surgery in elderly patients (previously independent and without cognitive impairment) with hip fracture caused by a fall.

  20. Análisis comparativo del VO 2máx estimado mediante las ecuaciones desarrolladas por Jackson et al y el American College of Sport Medicine en corredores de maratón

    Microsoft Academic Search

    Robinson Ramírez Vélez; Ricardo Antonio Agredo Zuñiga; José Guillermo Ortega Ávila; Viviana Andrea Dosman González; Carlos Alejandro López Alban

    2009-01-01

    BackgroundReduced cardiorespiratory function is an independent risk factor for mortality by all causes, but mainly for coronary heart disease. Nevertheless, there are many difficulties in evaluating it by exercise testing in the epidemiological context. Alternative forms of evaluation have therefore been suggested using non-exercise regression models.

  1. Detección de Virus por Serología y Plantas Indicadoras en el Tubérculo-Semilla y Plantas de Cultivo de Meristemos en Papa (Solanum tuberosum L.) var. Alfa

    Microsoft Academic Search

    Víctor Valenzuela-Herrera; Rafael Bujanos-Muñiz

    2003-01-01

    Viral detection in Alpha potato tubers from Tenango del Valle, State of Mexico, was performed by ELISA and indicator plants. Potato tubers were planted in the greenhouse, and the plants were kept in insect-proof cages; sap was extracted from top leaflets to mechanically inoculate indicator plants for Potato leafroll virus (PLRV), Potato virus A (PVA), Potato virus X (PVX), Potato

  2. Construcci\\'on de un reactor de nitruraci\\'on por plasma y simulaci\\'on de la distribuci\\'on de temperaturas

    E-print Network

    Molleja, Javier García; Feugeas, Jorge

    2014-01-01

    Ion nitriding and ion carburising by plasma discharges are promising techniques in order to modify the surface of austenitic steels. Plasma treatments improve mechanical properties, e.g., hardness and wear resistance. Indeed, these techniques do not change the natural austenitic properties, like corrosion resistance. Hidden parameters of the system are very important in order to do a good characterization and a good experimental reproducibility. In this work a new cathode design has been done and it is placed in a new plasma reactor. The electric field edge effect has been discarded in the austenitic samples thanks to a special lodging procedure. After an experimental validation of this new set-up, a numerical simulation has been carried out. Temperature distribution has been analyzed using the cathode-molecular nitrogen collision method. Under these assumptions, reactor cooling is a key issue. This simulation has been done after the resolution of the Laplace equation with the parallel Jacobi method. Finally,...

  3. Potencial forrajero del pasto salado Distichlis spicata L. (Greene) en ecosistemas costeros de Baja California Sur, México por el método de \\

    Microsoft Academic Search

    Arturo Escobar-Hernándezab; Enrique Troyo-Dieguez; José Luis García-Hernández; Hugo Hernández-Contreras; Bernardo Murillo-Amador; Raúl López-Aguilar

    Forage potential of the non-domesticated saltgrass Distichlis spicata (Gramineae) was determined by analyzing its chemical and productive variables using principal components analysis (PCA). Plants were collected from five sites amongst three locations, in areas including wetlands shores, centers, and beach. The results suggested that D. spicata is a rough, poorly digested forage, as explained by the statistical significance of the

  4. The effects of the porous buffer layer and doping with dysprosium on internal stresses in the GaInP:Dy/por-GaAs/GaAs(100) heterostructures

    SciTech Connect

    Seredin, P. V.; Gordienko, N. N.; Glotov, A. V.; Zhurbina, I. A.; Domashevskaya, E. P. [Voronezh State Technical University (Russian Federation); Arsent'ev, I. N., E-mail: arsentyev@mail.ioffe.ru; Shishkov, M. V. [Russian Academy of Sciences, Ioffe Physicotechnical Institute (Russian Federation)

    2009-08-15

    In structures with a porous buffer layer, residual internal stresses caused by a mismatch between the crystal-lattice parameters of the epitaxial GaInP alloy and the GaAs substrate are redistributed to the porous layer that acts as a buffer and is conducive to disappearance of internal stresses. Doping of the epitaxial layer with dysprosium exerts a similar effect on the internal stresses in the film-substrate structure.

  5. Epidemiologia y manifestaciones clinicas de la infeccion por especies de Nocardia en Tarragona, 1997 a 2008: Nocardia cyriacigeorgica es un patogeno emergente

    Microsoft Academic Search

    Olga Portolaa; Ramon Guitart; Frederic Gomez; Montserrat Olona; Francesc Vidal; Antoni Castro; Joan XXIII

    Introduction: Nocardia infections are uncommon, and the clinical experience with this pathogen is limited. Methods: We describe the clinical characteristics of a series of 22 patients (17 men) with Nocardia infection seen between 1997 and 2008. Results: Mean age was 63.2719.2 years. The most common clinical forms observed were lower respiratory tract infection (15\\/22, 68%, 8 bronchitis and 7 pneumonia)

  6. RECONOCIMIENTO DE COMANDOS POR VOZ CON MÁQUINAS DE SOPORTE VECTORIAL A TRAVÉS DE BANDAS ESPECTRALES Voice commands recognizing using support vector machines and spectral bands

    Microsoft Academic Search

    GERMÁN ANDRÉS MORALES; Ingeniero Electricista; RENÉ ALEXANDER BARRERA; Ingeniero Electrónico; JUAN JOSÉ MORA FLÓREZ; GRUPO DE INVESTIGACIÓN; SISTEMAS DE ENERGÍA

    2007-01-01

    In this paper a voice command recognizing methodology using Support Vector Machines (SVM) is proposed. This is an important task in autonomous and semi-autonomous systems, because it is a natural and useful interaction way, especially in situations where there are special limitations as low visibility, low or any possibility of physical contact, among others. As application example, voice registered signals

  7. Marcadores biológicos de estrés oxidativo. Distribución por edad y sexo de las concentraciones basales de MDA, NO y ácido úrico en niños y adolescentes de Maracaibo-Venezuela

    Microsoft Academic Search

    Souki A; Cano C; Mengual E; García D; Torres D; Almarza J; León L; Chávez Z; Molero E; Medina M; Félix Gómez

    2007-01-01

    Despite recognition of the detrimental effects of malondial- dehyde (MDA), nitric oxide (NO) and uric acid on oxidative stress, this hypothesis has not yet been tested in healthy chil- dren and adolescents. The objective of the present study was to describe the behavior of these parameters, in a sample of healthy children and adolescents from Maracaibo, Zulia State, Venezuela. After

  8. Use of medicinal plants among patients with diabetes mellitus type 2 in Morelos, Mexico (Uso de plantas medicinales por pacientes con diabetes mellitus tipo 2 en Morelos, México)

    Microsoft Academic Search

    Ofelia ROMERO-CERECERO; Hortensia REYES-MORALES; Lucía AGUILAR-SANTAMARÍA; Maira HUERTA-REYES; Jaime TORTORIELLO-GARCIA

    The aim of the study was the identification of the most frequently used plants by patients with DM2 with access to public health services in Morelos, Mexico. A questionnaire was employed with 259 patients with DM2 who regularly utilize medicinal plants as a complementary therapy, and another 259 patients who only use the pharmaceutical prescription in primary-care clinics from two

  9. ESTUDO RETROSPECTIVO DO TRATAMENTO CIRÚRGICO DO NEUROMA DE MORTON POR VIA PLANTAR RETROSPECTIVE STUDY OF SURGERY TREATMENT IN MORTONS NEUROMA BY PLANTAR APPROACH

    Microsoft Academic Search

    GUSTAVO GENNARI BARBOSA; GUSTAVO MALUF TIRADENTES; HELENCAR IGNÁCIO; GUARACY CARVALHO FILHO; ALCEU GOMES CHUEIRE

    SUMMARY With an eye to evaluate the surgical technic efficiency - neurectomy by plantar approach -, 19 patients with Mortons neuroma were submitted to this treatment. Therefore, were 19 neuroma patients including 84,3% female, 15,7% male; 31,5% in second intermeta- tarsal space, 68,5% in third; 47,3% on right side and 52,7% on left side. The surgery was made by plantar

  10. ANÁLISIS POR CG\\/EM DE HIDROCARBUROS Y COMPUESTOS TERPÉNICOS EN LAS ESPECIES Euphorbia caracasana Boiss Y Euphorbia cotinifolia L. (EUPHORBIACEAE)

    Microsoft Academic Search

    JANNE ROJAS; ANTONIO MORALES; GINA MECCIA; MARÍA RONDÓN

    In the present investigation the chemical composition of several fractions con- taining hydrocarbons and terpenoids type compounds of E. caracasana Boiss and E. cotinifolia L. collected in two different places in Merida State, Venezuela, ana- lyzed by GC\\/MS is being described. Trans-phytol, an unsaturated chain alcohol and several either saturated or unsaturated (C16 - C20) fatty acid methyl esters, were

  11. artigo original Substituição da Insulina NPH por Insulina Glargina em Uma C o o rte de Pacientes Diabéticos: Estudo Observa c i o n a l

    Microsoft Academic Search

    FREDERICOF. R. MAIA; Universitário São José

    2007-01-01

    Switching of NPH Insulin to Glargine Therapy in a Cohort of Poorly Con- trolled Diabetic Patients: Observational Study. This study examined the impact of insulin glargine introduction in basal-bolus thera- py in type 1 and type 2 diabetic patients with inadequate metabolic control (A1c > 6.9%) using previous NPH insulin regime. In this uncontrolled, retrospective study, 49 patients (28F\\/21M), average

  12. Atlas geographico de la America septentrional y meridional ... Por ... Thomas Lopez pensionista de S.M. en la corte de Paris an?o de 1758 

    E-print Network

    Lo?pez de Vargas Machuca, Toma?s

    1758-01-01

    composed of a split sheath and blade The flowered unit is the spikelet composed of one or more florets. FORB ? Any herb other than a grass. LEGUME ? Herbs or woody plants with usually showy bonnet-like or butterfly-shaped flowers, alternate mostly compound..., INTRODUCED ? Not native to North America. It may be desirable or undesirable for use. WILDLIFE VALUES ? Are based on palatability of the plants to wildlife of? an economic importance ? deer, antelope, turkey, quail and dove. The plants were rated from...

  13. Relación entre Condiciones de Suelo y Manejo de Huertas de Nogal Pecanero Carya illinoensis Koch con la Dinámica de la Pudrición Texana, causada por Phymatotrichum omnivorum Duggar

    Microsoft Academic Search

    José Alfredo Samaniego-Gaxiola; Teodoro Herrera-Pérez; Jesús Santamaría-César

    2001-01-01

    From 1995 to 1998, 26 plots of three ha each were selected in commercial walnut orchards. The objective of the survey was to relate the dynamics of Phymatotrichum root rot with orchard management and soil condition. The management variables were: age of the trees, plantation distance, trees\\/ha, pest management, number of irrigations and incidence of diseased trees (in 1995); for

  14. Atuação da Fisioterapia sobre o Tempo de Internação dos Neonatos Pré-termo Acometidos por Distúrbios Respiratórios na UTI Neo natal do Hospital Universitário São Francisco de Paula

    Microsoft Academic Search

    Ana Paula Ribeiro; Clarissa Bachini Barros; Débora Crochemore Bettin; Elenice Maahs Piper; Gabriela Lemos dos Santos; Gilcéli da Silva Fernandes; Katiúscia Naparo Mirapalhete; Lara Vergara Radmann; Flaviano Moreira da Silva

    Resumo Introdução: No Brasil observa-se um alto índice de re- cém-nascidos (RN's) pré-termo. Os RN's que apresentam complicações peri ou pós-natais, na maioria das vezes correm algum risco de morte podendo apresentar hipóxia neonatal, baixo peso ao nascer, distúrbios neurológicos e alterações respiratórias sendo encaminhados a Unida- des de Terapia Intensiva Neonatais (UTIN). Objetivo: Comparar o tempo de internação dos

  15. Degradación enzimática de celulosa por actinomicetos termófilos: aislamiento, caracterización y determinación de la actividad celulolítica Enzymatic degradation of cellulose for thermophilic actinomycete: isolation, characterization and cellulolytic activity determination

    Microsoft Academic Search

    Pablo Ramírez; Juana María Coha

    2003-01-01

    One hundred and forty five cellulolytic thermophilic actinomycete strains were isolated from 71 compost, soil, hay and dung samples. Streptomyces sp. (50,63%), Thermomonospora curvata (15,82%), T. chromogena (13,92%), and other species were identified. Endoglucanase, exoglucanase and ?-glucosidase activities were evaluated from 10 cellulolytic actinomycete strains. Among these the Streptomyces sp. 7CMC10 strain showed the biggest activity levels corresponding to 20,14;

  16. Aplicación de los factores de participación y del método de MW-Milla en la asignación de cargos por uso de redes de transmisión en mercados de electricidad

    Microsoft Academic Search

    L. Alba-Gómez; J. H. Tovar-Hernández; G. Gutiérrez-Alcaraz

    Use of net work al lo ca tion costs by shift fac tors and MW-Mile method is re ported in this pa - per. Con ven tional shift fac tors are com puted based on DC power flow. DC power flow re - quires to se lect ing a slack bus in or der to avoid ma trix sin gu

  17. Almacenamiento de presas por el Alcaudón real (Lanius excubitor) en la Serena (Badajoz) y la Sierra de Cabo de Gata (Almería)

    Microsoft Academic Search

    Ángel Hernández; José María Salgado

    1993-01-01

    Durant una visita a la Serena (Badajoz, 29.12.89) i a la serra de Cabo de Gata (Almería, 07.01.91) es van recol·lectar 55 preses empalades de botxí. A la Serena la majoria de les preses eren rèptils i aràcnids (n=25). Totes les preses estaven empalades en filferro espinós, probablement a causa de la manca d'arbres i arbusts a la zona. A

  18. Mass transfer and phenolic profile of strawberries upon refrigerated osmodehydration Transferencia de masa y perfil fenólico de las fresas cuando son osmo-deshidratadas por refrigeración

    Microsoft Academic Search

    G. Blanda; L. Cerretani; A. Cardinali; E. Boselli; A. Bendini

    2010-01-01

    Whole strawberries were osmodehydrated at low temperature (5 °C) in a sucrose syrup (500 g\\/kg of solution) for different processing times (24, 48, 72, 96, and 120 h); after the treatment, water loss, solid gain, and weight reduction were recorded. Next, the osmodehydrated samples were frozen, and after a 5-month storage at ?18 °C they were analyzed for drip loss, titratable acidity,

  19. Listado de cursos de verano por temtica II CURSO INTERNACIONAL DE CUERDA Y PIANO DE LA UNIVERSIDAD DE ALICANTE. MODALIDAD ACTIVO

    E-print Network

    Escolano, Francisco

    EDICIÓN ESTRATEGIAS PARA MEJORAR EL BIENESTAR PERSONAL: INTELIGENCIA EMOCIONAL Y LA PSICOLOGÍA POSITIVA PATRIMONIO ARTÍSTICO CULTURAL Ciencias de la educación 3ª JORNADA DE INNOVACIÓN EDUCATIVA: LAS INTELIGENCIAS EMOCIONAL, COACHING, CORPORALIDAD, COMUNICACIÓN Y LIDERAZGO LITERATURA INFANTIL Y JUVENIL. TEXTOS

  20. preparado por Alvaro Veiga, PUC-Rio, alvf@ele.puc-rio.br Modelos estocsticos para avaliao de contratos deModelos estocsticos para avaliao de contratos de

    E-print Network

    Liu, I-Shih

    ); Gestão de ativos e passivos para o setor de petróleo (Petrobras, 2009-2011); Cálculo de reserva IBNR petróleo (Petrobras, 2008- 2009); Indicadores de qualidade de geração (IPEA, 2011-2012); Modelo de

  1. EVALUACIÓN E INTERVENCIÓN PSICOLÓGICA EN UN CASO DE TRASTORNO ADAPTATIVO MIXTO CON ANSIEDAD Y ESTADO DE ÁNIMO DEPRESIVO EN UNA MUJER CON RECIDIVA POR CÁNCER DE MAMA

    Microsoft Academic Search

    Paula Andrea Barraza; Facultad de Psicología

    2004-01-01

    Adjustment Disorder is at present the most frequently diagnosed psychopathology among cancer patients. In this article a case report of an adjustment disorder with mixed mood, anxiety and depressed mood, in a woman with breast cancer recurrence, is presented. A brief psychological intervention, in the crisis intervention model, cognitive - behavioural oriented is proposed. Problems expressed at physiological, motor and

  2. Aguaita, No. 15-16, diciembre 2006 junio 2007, p. 176-192 De Kinshasa a Cartagena, pasando por Pars: itinerarios de una "msica negra", la

    E-print Network

    Paris-Sud XI, Université de

    del soukous, es el fruto de otro viaje, el que trajo el son cubano hacia África, en particular las dos. Más concretamente, su éxito está vinculado a su ambigüedad: al mismo tiempo entrada en la modernidad y

  3. Cuatro estudiantes de la Universidad de Alicante reciben por primera vez las becas Aitor Espinosa (Ingeniera de Caminos), Irene Finestrat (Estudios Ingleses), Manuel

    E-print Network

    Escolano, Francisco

    aula sino también a través de viajes de estudio, en trabajo de campo y experiencias académicas determinados requisitos y hemos tenido muy poquito tiempo para reunirlos todos. ¿ Como te sientes al ser el

  4. El acceso al episcopado en la Monarqua Hispnica (1789-1800)* La ltima dcada del siglo XVIII, clave por las repercusiones polticas del primer ciclo

    E-print Network

    Paris-Sud XI, Université de

    del siglo XVIII, con el objetivo de comprobar hasta qué punto los factores de uno u otro origen, Francis: Kingship. The Politics of Enchantment, Blackwell, Malden/Oxford/Carlton, 2006, pp. 140-142. 2 AGS

  5. CÁTIONS TROCÁVEIS, CAPACIDADE DE TROCA DE CÁTIONS E SATURAÇÃO POR BASES EM SOLOS BRASILEIROS ADUBADOS COM COMPOSTO DE LIXO URBANO1

    Microsoft Academic Search

    Cassio Hamilton Abreu Jr; Takashi Muraoka; Fernando Carvalho Oliveira

    The use of urban organic solid waste compost as organic fertilizer is an important alternative to solid waste management. This waste has been applied to agricultural lands because of the benefits in relation to soil chemical properties. A greenhouse experiment was conducted to investigate the effects of the application of urban waste compost, at the rate of 30 g dm

  6. GENERACIÓN DE PAGOS POR CERTIFICADOS DE REDUCCIÓN DE EMISIONES, BAJO EL MECANISMO DE DESARROLLO LIMPIO PARA CERCOS VIVOS EN LA CUENCA ALTA DEL R.PASTO

    Microsoft Academic Search

    JAVIER SABOGAL-AGUILAR

    2007-01-01

    Resumen: Este artículo es resultado del proyecto de Restauración y Protección de Agroecosistemas Estratégicos en la Captura de Carbono, Municipio de Pasto, Nariño. Explora la posibilidad que tienen las comunidades y propietarios de pequeños predios de la Cuenca Alta del Río Pasto de contribuir a la mitigación del Cambio Climático a la vez que obtienen ingresos monetarios y otros beneficios

  7. Precariedad laboral de los jóvenes y segmentación por la edad en Rhône-Alpes : análisis global de las condiciones de empleo y estudio específico los principales sectores terciarios

    Microsoft Academic Search

    Irune López-Pérez; Jon Bernat Zubiri-Rey

    2012-01-01

    El periodo neoliberal ha supuesto una regresión en la distribución de excedentes de la economía mercantil. Este proceso se da a escala global y entre clases sociales en cada ámbito localizado, generando una creciente segmentación sociolaboral que se reimpulsa a partir de mediados de los 90 mediante nuevas regulaciones y diversos formatos sectoriales de precarización laboral. La región francesa de

  8. Avaliação da qualidade dos registros de enfermagem no prontuário por meio da auditoria Evaluation of the quality of nursing documentation though the review of patient medical records

    Microsoft Academic Search

    Vanessa Grespan Setz; Maria D'Innocenzo

    2009-01-01

    Objective: To evaluate the quality of nursing documentation on medical records of patients from a university hospital in São Paulo, Brazil. Methods: A retrospective descriptive study was used to conduct the study. Four hundred and twenty four medical records of patients from medical and surgical units were reviewed from November 2006 to January 2007. The medical records were from patients

  9. PROBABILIDADE DE RUÍNA COMO CRITÉRIO PARA DEFINIR O COEFICIENTE DE SEGURANÇA A SER USADO NA PREVISÃO DA CARGA ADMISSÍVEL DE FUNDAÇÕES POR ESTACAS

    Microsoft Academic Search

    Nelson Aoki; Mauro Leandro Menegotto; José Carlos; A. Cintra

    Resumo - A atual filosofia da Norma Brasileira NBR 6122\\/1996 de Projeto e Execução de Fundações baseia-se no conceito de coeficiente de segurança global ou parcial. O critério atual para a escolha do coeficiente de segurança global, quando a capacidade de carga da estaca é estimada a partir de métodos empíricos, é adotar o valor proposto pelo autor da teoria.

  10. Problemas de topologia. Espacios normales (Hoja 5) 1. Probar que la linea de Sorgenfrey Rs es normal, pero Rs Rs no lo es, (por tanto

    E-print Network

    Martín Peinador, Elena

    Problemas de topolog´ia. Espacios normales (Hoja 5) 24-IV-2008 1. Probar que la linea de Sorgenfrey. Probar que todo subespacio cerrado de un espacio normal es normal. Sin embargo la "normalidad" no es propiedad hereditaria. 3. Demostrar que si en un espacio topol´ogico normal (X, T) se tienen tres cerrados C

  11. 3.2. Estudios de postgrado Durante el ao acadmico 2011-12, el RD 1393/2007, de 29 de octubre, por

    E-print Network

    Oro, Daniel

    Química Teórica y Modelización Computacional, Doctorado de Neurociencias, Doctorado de Nutrición Humana Universitario en Neurociencias. Máster Universitario en Nutrición Humana y Calidad de los Alimentos. Rama de

  12. Prevalencia de la infección por el virus del papiloma humano en mujeres con citologías anormales del cérvix uterino y factores de riesgo asociados a la infección

    Microsoft Academic Search

    Daniel Tena; Nuria Garrido; Juan José Delgado; José Manuel Menéndez; Juan Romanyk; María del Rosario González; Álvaro Zapico; María Beltrán

    2006-01-01

    ObjectivesTo determine the prevalence of human papillomavirus (HPV) infection in women with an abnormal pap smear of the uterine cervix and to determine the risk factors associated with HPV infection.

  13. Aplicaciones de la citologia oral por raspado (exfoliativa) en el cáncer y precáncer oral Applications of the oral scraped (exfoliative) cytology in oral cancer and precancer

    Microsoft Academic Search

    Acha A; Ruesga MT; Rodríguez MJ; Martínez de Pancorbo

    Scraped (exfoliative) cytology is a simple and harmless proce- dure, which has been a controversial technique according to its real validity in oral pathology. Lately it has re-emerged due to its application in oral precancer and cancer as a diagnostic and predictive method as well as for monitoring patients. New diag- nostic techniques have been developed, such as \\

  14. Alteraciones patológicas producidas por la inoculación subcutánea de los extractos alergénicos de Blomia tropicalis y Dermatophagoides siboney en ratas y ratones

    Microsoft Academic Search

    Fuentes D; González Navarro; González Torres; Aldana L; L abrada A; Finca Tirabeque

    2003-01-01

    Pathological changes due to subcutaneal adminis- tration of Dermatophagoides siboney and Blomia tropicalis allergenic extracts in rats and mice. The allergenic extracts are used in immunotherapy treatments, because they are able to induce immunologic changes in the allergic response, signifi- cantly reducing the clinical symptoms of the illness. These ther- apeutic preparations are applied directly to man. The objective of

  15. Ambiente de Ensino a Distância Aplicado Para Treinamento em Radiologia e Diagnóstico por Imagens: Estudo de Caso em Lesão Focal de Fígado

    Microsoft Academic Search

    Marília Guimarães Pinheiro; Rodrigo Donizete; Santana de Pádua

    This paper presents a computational feature environment to support the training and evaluation of medical resident in radiology and diagnostic images by using E-learning. It used the Moodle, which is free-open source software, with adjustments for viewing medical images. The structure of the tests created was inspired in \\

  16. Evaluación endoscópica de hemorragia pulmonar inducida por el ejercicio en equinos de polo Endoscopic evaluation of Exercise Induced Pulmonary Hemorrhage in Polo Ponies

    Microsoft Academic Search

    G. MORAN; R. CARRILLO; B. CAMPOS; C. GARCIA; Olivares N

    2003-01-01

    SUMMARY The objective of the present study was to determine the presence of Exercise Induced Pulmonary Hemorrhage (EIPH) by endoscopic examination in polo ponie after competition. Thirty nine polo horses in training were used in this study. The animal were classified by the number of polo seasons they had played (two seasons and three or more) and by sex independently

  17. Efeito tópico de iodo-povidona 0,5% comparado com natamicina 5% em ceratites fúngicas causadas por Fusarium solani em um modelo animal de coelhos: estudo piloto

    Microsoft Academic Search

    Lauro Augusto de Oliveira; Thales Takeo Takata; Olga Fischman Gompertz; Luciene Barbosa de Sous; Mark J. Mannis

    Purpose: To evaluate the efficacy of topical administration of 0.5% povidone-iodine in experimental Fusarium solani keratitis in rabbits. Methods: Fungal keratitis caused by Fusarium solani was induced in the right eye of 24 New Zealand rabbits. The rabbits were randomly divided into 3 different treatment groups: Group I (povidone-iodine) - treated with topical 0.5% povidone-iodine; Group II (natamycin) - treated

  18. ESTUDIO PARA DETERMINAR LA FACTIBILIDAD DEL REEMPLAZO DEL ERP MICROSIP POR EL ERP SAP BUSINESS ONE PARA RESOLVER LAS NECESIDADES DE INFORMACIÓN FINANCIERA DE GRUPO URSUA

    Microsoft Academic Search

    Jorge Ricardo Vásquez Sánchez

    2009-01-01

    Hoy en día el manejo de la información, dentro de una organización, constituye una parte crucial para el desarrollo de las distintas actividades empresariales, y representa la base, en la que se sustenta, una eficaz toma de decisiones gerenciales, que permiten conducir con éxito a las organizaciones en mercados altamente competitivos.

  19. Fundamentos del rechazo a la transfusión sanguínea por los Testigos de Jehová. Aspectos ético-legales y consideraciones anestésicas en su tratamiento

    Microsoft Academic Search

    A. Pérez Ferrer; E. Gredilla; J. de Vicente; J. García Fernández; F. Reinoso Barbero

    Summary The refusal of Jehovah's Witnesses to agree to blood or blood product transfusion based on religious beliefs is one of the most challenging conflictive issues health care givers have to face today. Such conflict is a by product of the ideological and religious diversity in society today. The perioperative care of such patients constitutes a genuine challenge for anesthesiologists

  20. Análise de Associação por Todo o Genoma para Identificar Locos Relacionados ao Lucro Líquido, à Vida Produtiva e ao Escore de Células Somáticas na raça Jersey1

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A genome scan was conducted in the US Jersey population to identify QTL affecting net merit, productive life, and somatic cell score. Data used at this study were DHI records from the national database of the Animal Improvement Programs Laboratory, USDA (Beltsville, MD). DNA was acquired for 2,380 a...