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Sample records for por moraxella catarrhalis

  1. Comparison of Restriction Enzymes for Pulsed-Field Gel Electrophoresis Typing of Moraxella catarrhalis

    PubMed Central

    Puig, Carmen; Domenech, Arnau; Liñares, Josefina; Ardanuy, Carmen

    2013-01-01

    NotI, the most prevalent restriction enzyme used for typing Moraxella catarrhalis, failed to digest genomic DNA from respiratory samples. An improved pulsed-field gel electrophoresis (PFGE) methodology determined SpeI as the best choice for typing this bacterial species, with a good restriction of clinical samples and a good clustering correlation with NotI. PMID:23678064

  2. Moraxella catarrhalis Expresses a Cardiolipin Synthase That Impacts Adherence to Human Epithelial Cells

    PubMed Central

    Buskirk, Sean W.

    2014-01-01

    The major phospholipid constituents of Moraxella catarrhalis membranes are phosphatidylglycerol, phosphatidylethanolamine, and cardiolipin (CL). However, very little is known regarding the synthesis and function of these phospholipids in M. catarrhalis. In this study, we discovered that M. catarrhalis expresses a cardiolipin synthase (CLS), termed MclS, that is responsible for the synthesis of CL within the bacterium. The nucleotide sequence of mclS is highly conserved among M. catarrhalis isolates and is predicted to encode a protein with significant amino acid similarity to the recently characterized YmdC/ClsC protein of Escherichia coli. Isogenic mclS mutant strains were generated in M. catarrhalis isolates O35E, O12E, and McGHS1 and contained no observable levels of CL. Site-directed mutagenesis of a highly conserved HKD motif of MclS also resulted in a CL-deficient strain. Moraxella catarrhalis, which depends on adherence to epithelial cells for colonization of the human host, displays significantly reduced levels of adherence to HEp-2 and A549 cell lines in the mclS mutant strains compared to wild-type bacteria. The reduction in adherence appears to be attributed to the absence of CL. These findings mark the first instance in which a CLS has been related to a virulence-associated trait. PMID:24142255

  3. Development of a LacZ-based transcriptional reporter system for use with Moraxella catarrhalis.

    PubMed

    Evans, Amanda S; Pybus, Christine; Hansen, Eric J

    2013-03-01

    The lack of a transcriptional reporter system for use in Moraxella catarrhalis has hindered studies of gene regulation in this pathogen. PCR and recombinant DNA methods were used to insert a multicloning site (MCS) and promoterless full-length Escherichia coli lacZ gene, flanked by transcriptional terminators both immediately upstream and downstream, into the M. catarrhalis recombinant plasmid pWW115. Insertion into the MCS in the newly constructed plasmid pASE222 of M. catarrhalis promoter regions controlled by either a repressor (i.e., NsrR) or activator (i.e., PhoB) yielded transcriptional fusion constructs that were appropriately responsive to signal inputs dependent on the host strain genotype, as measured quantitatively by means of a Miller ?-galactosidase assay. The transcriptional reporter plasmid pASE222 should prove to be a useful tool for rapid screening of factors affecting gene expression in M. catarrhalis. PMID:23219721

  4. Prevalence and resistance pattern of Moraxella catarrhalis in community-acquired lower respiratory tract infections

    PubMed Central

    Shaikh, Safia Bader Uddin; Ahmed, Zafar; Arsalan, Syed Ali; Shafiq, Sana

    2015-01-01

    Introduction Moraxella catarrhalis previously considered as commensal of upper respiratory tract has gained importance as a pathogen responsible for respiratory tract infections. Its beta-lactamase-producing ability draws even more attention toward its varying patterns of resistance. Methods This was an observational study conducted to evaluate the prevalence and resistance pattern of M. catarrhalis. Patients aged 20–80 years admitted in the Department of Chest Medicine of Liaquat National Hospital from March 2012 to December 2012 were included in the study. Respiratory samples of sputum, tracheal secretions, and bronchoalveolar lavage were included, and their cultures were followed. Results Out of 110 respiratory samples, 22 showed positive cultures for M. catarrhalis in which 14 were males and eight were females. Ten samples out of 22 showed resistance to clarithromycin, and 13 samples out of 22 displayed resistance to erythromycin, whereas 13 showed resistance to levofloxacin. Hence, 45% of the cultures showed resistance to macrolides so far and 59% showed resistance to quinolones. Conclusion Our study shows that in our environment, M. catarrhalis may be resistant to macrolides and quinolones; hence, these should not be recommended as an alternative treatment in community-acquired lower respiratory tract infections caused by M. catarrhalis. However, a study of larger sample size should be conducted to determine if the recommendations are required to be changed. PMID:26261422

  5. Non-transpeptidase binding arylthioether ?-lactams active against Mycobacterium tuberculosis and Moraxella catarrhalis.

    PubMed

    Beck, Tim N; Lloyd, Dina; Kuskovsky, Rostislav; Minah, Jeanette; Arora, Kriti; Plotkin, Balbina J; Green, Jacalyn M; Boshoff, Helena I; Barry, Clifton; Deschamps, Jeffrey; Konaklieva, Monika I

    2015-02-01

    The prevalence of drug resistance in both clinical and community settings as a consequence of alterations of biosynthetic pathways, enzymes or cell wall architecture is a persistent threat to human health. We have designed, synthesized, and tested a novel class of non-transpeptidase, ?-lactamase resistant monocyclic ?-lactams that carry an arylthio group at C4. These thioethers exhibit inhibitory and cidal activity against serine ?-lactamase producing Mycobacterium tuberculosis wild type strain (Mtb) and multiple (n=8) ?-lactamase producing Moraxella catarrhalis clinical isolates. PMID:25549898

  6. C4-Alkylthiols with activity against Moraxella catarrhalis and Mycobacterium tuberculosis

    PubMed Central

    Kostova, Maya B.; Myers, Carey J.; Beck, Tim N.; Plotkin, Balbina J.; Green, Jacalyn M.; Boshoff, Helena I.M.; Barry, Clifton E.; Deschamps, Jeffrey R.; Konaklieva, Monika I.

    2013-01-01

    Antimicrobial resistance represents a global threat to healthcare. The ability to adequately treat infectious diseases is increasingly under siege due to the emergence of drug-resistant microorganisms. New approaches to drug development are especially needed to target organisms that exhibit broad antibiotic resistance due to expression of ?-lactamases which is the most common mechanism by which bacteria become resistant to ?-lactam antibiotics. We designed and synthesized 20 novel monocyclic ?-lactams with alkyl- and aryl-thio moieties at C4, and subsequently tested these for antibacterial activity. These compounds demonstrated intrinsic activity against serine ?-lactamase producing Mycobacterium tuberculosis wild type strain (Mtb) and multiple (n = 6) ?-lactamase producing Moraxella catarrhalis clinical isolates. PMID:22014754

  7. Expression of the Oligopeptide Permease Operon of Moraxella catarrhalis Is Regulated by Temperature and Nutrient Availability.

    PubMed

    Jones, Megan M; Murphy, Timothy F

    2015-09-01

    Moraxella catarrhalis causes otitis media in children and exacerbations of chronic obstructive pulmonary disease in adults. Together, these two conditions contribute to enormous morbidity and mortality worldwide. The oligopeptide permease (opp) ABC transport system is a nutritional virulence factor important for the utilization of peptides. The substrate binding protein OppA, which binds peptides for uptake, is a potential vaccine antigen, but little was known about the regulation of gene expression. The five opp genes oppB, oppC, oppD, oppF, and oppA are in the same open reading frame. Sequence analysis predicted two promoters, one located upstream of oppB and one within the intergenic region between oppF and oppA. We have characterized the gene cluster as an operon with two functional promoters and show that cold shock at 26°C for ? 0.5 h and the presence of a peptide substrate increase gene transcript levels. Additionally, the putative promoter upstream of oppA contributes to the transcription of oppA but is not influenced by the same environmental cues as the promoter upstream of oppB. We conclude that temperature and nutrient availability contribute to the regulation of the Opp system, which is an important nutritional virulence factor in M. catarrhalis. PMID:26099587

  8. 1,2,3-Triazole pharmacophore-based benzofused nitrogen/sulfur heterocycles with potential anti-Moraxella catarrhalis activity.

    PubMed

    Mara?i?, Silvija; Kraljevi?, Tatjana Gazivoda; Paljetak, Hana ?ip?i?; Peri?, Mihaela; Matijaši?, Mario; Verbanac, Donatella; Cetina, Mario; Rai?-Mali?, Silvana

    2015-12-01

    Versatile 1,2,3-triazole pharmacophore-based benzofused heterocycles containing halogen-substituted aromatic (9-17 and 25-28), 7-substituted coumarin (18-23 and 29-30) or penciclovir-like subunit (31a,b-38a) were designed and synthesized to evaluate their antibacterial activities against selected Gram-positive and Gram-negative bacteria. Hybridization approach using environmentally friendly Cu(I)-catalyzed click reaction under microwave irradiation was adopted in the synthesis of regioselective 1,4-disubstituted 1,2,3-triazole tethered heterocycles (9-23 and 25-30), while post-N-alkylation of NH-1,2,3-triazoles afforded both 2,4- (31a-38a) and 1,4-disubstituted (31b-33b, 35b-37b) 1,2,3-triazole regioisomers. The compounds 18-23 and 25-30 revealed fluorescence in the violet region of the visible spectrum with a strong influence of phenyl spacer in 25-30 on both wavelength and emission intensity. Fusion of selected subunits led to new hybrid architecture, benzothiazole-1,2,3-triazole-coumarin 29 that demonstrated extremely narrow spectrum activity towards fastidious Gram-negative bacteria Moraxella catarrhalis. Selected hybrid showed the potency against Moraxella catarrhalis (MIC?0.25?g/mL) comparable to that of reference antibiotic azithromycin, which suggested that further investigations are necessary to optimize this potential hit compound as a new anti-Moraxella catarrhalis agent. PMID:26578325

  9. Growth of Moraxella catarrhalis with human transferrin and lactoferrin: expression of iron-repressible proteins without siderophore production.

    PubMed Central

    Campagnari, A A; Shanks, K L; Dyer, D W

    1994-01-01

    Moraxella (Branhamella) catarrhalis, a mucosal pathogen closely related to Neisseria species, is a prominent cause of otitis media in young children and lower respiratory tract infections in adults. In this study, we investigated whether M. catarrhalis can compete for iron bound to human transferrin or human lactoferrin in a manner similar to that utilized by Neisseria meningitidis and Neisseria gonorrhoeae. Our studies demonstrated that M. catarrhalis obtains iron from these serum carrier proteins and also maintains growth with ferric nitrate in vitro. Furthermore, we report that when M. catarrhalis is grown under iron-limited conditions, the bacteria express new outer membrane proteins that are not detected in membranes of organisms cultured in an iron-rich environment. We have shown that these are iron-repressible proteins since they are not induced by other environmental stresses and the expression of these proteins is repressed when a source of iron is provided for iron-limited bacteria. The iron-repressible proteins are expressed in the absence of any detectable siderophore production. These iron-repressible proteins may be important for the acquisition and utilization of iron in vivo, which could allow M. catarrhalis to colonize and survive on human mucosal surfaces. Images PMID:7927771

  10. Synthesis and characterization of lipooligosaccharide-based conjugate vaccines for serotype B Moraxella catarrhalis.

    PubMed

    Yu, Shengqing; Gu, Xin-Xing

    2005-05-01

    Moraxella catarrhalis is an important cause of otitis media in children and respiratory tract infections in the elderly. Lipooligosaccharide (LOS) is a major surface antigen of the bacterium that elicits bactericidal antibodies. Serological studies show that three major LOS types (A, B, and C) have been identified among clinical isolates. Our previous studies demonstrated that the type A LOS-based conjugates were immunogenic in animals. In this study, LOS from type B strain 26397 was detoxified and conjugated to tetanus toxoid (TT) or a cross-reactive mutant (CRM) of diphtheria toxin to form detoxified LOS (dLOS)-TT and dLOS-CRM, respectively, as vaccine candidates. The molar ratios of dLOS to TT and CRM in the conjugates were 43:1 and 19:1, respectively, while both weight ratios were around 0.9. The antigenicity of the conjugates was similar to that of the LOS, as determined by enzyme-linked immunosorbent assay using a rabbit antiserum to strain 26397. Subcutaneous immunization with each conjugate elicited a 180- to 230-fold rise of serum anti-LOS immunoglobulin G in mice and >2,000-fold rise in rabbits. In addition, both mouse and rabbit antisera showed elevated complement-mediated bactericidal activity against the homologous strain, and a representative rabbit antiserum showed bactericidal activity against nine of twelve clinical isolates studied. The bactericidal activity of the rabbit antiserum can be fully inhibited by the type B LOS but not the A or C LOS. These results indicate that the type B LOS-based conjugates can be used as vaccine components for further investigation. PMID:15845482

  11. Moraxella catarrhalis AcrAB-OprM Efflux Pump Contributes to Antimicrobial Resistance and Is Enhanced during Cold Shock Response

    PubMed Central

    Bernhard, Sara; Aebi, Christoph

    2015-01-01

    Moraxella catarrhalis is a common pathogen of the human respiratory tract. Multidrug efflux pumps play a major role in antibiotic resistance and virulence in many Gram-negative organisms. In the present study, the role of the AcrAB-OprM efflux pump in antibiotic resistance was investigated by constructing mutants that lack the acrA, acrB, and oprM genes in M. catarrhalis strain O35E. We observed a moderate (1.5-fold) decrease in the MICs of amoxicillin and cefotaxime and a marked (4.7-fold) decrease in the MICs of clarithromycin for acrA, acrB, and oprM mutants in comparison with the wild-type O35E strain. Exposure of the M. catarrhalis strains O35E and 300 to amoxicillin triggered an increased transcription of all AcrAB-OprM pump genes, and exposure of strains O35E, 300, and 415 to clarithromycin enhanced the expression of acrA and oprM mRNA. Inactivation of the AcrAB-OprM efflux pump genes demonstrated a decreased ability to invade epithelial cells compared to the parental strain, suggesting that acrA, acrB, and oprM are required for efficient invasion of human pharyngeal epithelial cells. Cold shock increases the expression of AcrAB-OprM efflux pump genes in all three M. catarrhalis strains tested. Increased expression of AcrAB-OprM pump genes after cold shock leads to a lower accumulation of Hoechst 33342 (H33342), a substrate of AcrAB-OprM efflux pumps, indicating that cold shock results in increased efflux activity. In conclusion, the AcrAB-OprM efflux pump appears to play a role in the antibiotic resistance and virulence of M. catarrhalis and is involved in the cold shock response. PMID:25583725

  12. A mutation affecting expression of a major outer membrane protein of Moraxella catarrhalis alters serum resistance and survival in vivo.

    PubMed

    Helminen, M E; Maciver, I; Paris, M; Latimer, J L; Lumbley, S L; Cope, L D; McCracken, G H; Hansen, E J

    1993-11-01

    A major outer membrane protein (CopB) of Moraxella catarrhalis is a target for antibodies that enhance clearance of this organism from the lungs of mice. A mini-Tn10kan transposon was inserted into the cloned copB gene from M. catarrhalis O35E, and an isogenic mutant unable to express the CopB protein was constructed by transforming this mutated gene into the wild-type strain. The mutant grew at the same rate as the wild-type parent strain in broth. Unlike the serum-resistant parent strain, this mutant was sensitive to killing by normal human serum, and its ability to survive and grow in the lungs of animals was impaired. Genetic restoration of CopB protein expression resulted in the simultaneous acquisition of wild-type levels of serum resistance and the ability to resist pulmonary clearance in vivo. Thus, the CopB protein of M. catarrhalis may be important in the interaction between this organism and the defense mechanisms of the respiratory tract. PMID:8228353

  13. Isolation and Characterization of Two Proteins from Moraxella catarrhalis That Bear a Common Epitope

    PubMed Central

    McMichael, John C.; Fiske, Michael J.; Fredenburg, Ross A.; Chakravarti, Deb N.; VanDerMeid, Karl R.; Barniak, Vicki; Caplan, Jeffrey; Bortell, Eric; Baker, Steven; Arumugham, Rasappa; Chen, Dexiang

    1998-01-01

    The UspA1 and UspA2 proteins of Moraxella catarrhalis are potential vaccine candidates for preventing disease caused by this organism. We have characterized both proteins and evaluated their vaccine potential using both in vitro and in vivo assays. Both proteins were purified from the O35E isolate by Triton X-100 extraction, followed by ion-exchange and hydroxyapatite chromatography. Analysis of the sequences of internal peptides, prepared by enzymatic and chemical cleavage of the proteins, revealed that UspA1 and UspA2 exhibited distinct structural differences but shared a common sequence including an epitope recognized by the monoclonal antibody 17C7. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), purified UspA1 exhibited a molecular weight of approximately 350,000 when unheated and a molecular weight of 100,000 after being heated for 10 min at 100°C. In contrast, purified UspA2 exhibited an apparent molecular weight of 240,000 by SDS-PAGE that did not change with the length of time of heating. Their sizes as determined by gel filtration were 1,150,000 and 830,000 for UspA1 and UspA2, respectively. Preliminary results indicate the proteins have separate functions in bacterial pathogenesis. Purified UspA1 was found to bind HEp-2 cells, and sera against UspA1, but not against UspA2, blocked binding of the O35E isolate to the HEp-2 cells. UspA1 also bound fibronectin and appears to have a role in bacterial attachment. Purified UspA2, however, did not bind fibronectin but had an affinity for vitronectin. Both proteins elicited bactericidal antibodies in mice to homologous and heterologous disease isolates. Finally, mice immunized with each of the proteins, followed by pulmonary challenge with either the homologous or a heterologous isolate, cleared the bacteria more rapidly than mock-immunized mice. These results suggest that UspA1 and UspA2 serve different virulence functions and that both are promising vaccine candidates. PMID:9712790

  14. Phenotypic Effect of Isogenic uspA1 and uspA2 Mutations on Moraxella catarrhalis 035E

    PubMed Central

    Aebi, Christoph; Lafontaine, Eric R.; Cope, Leslie D.; Latimer, Jo L.; Lumbley, Sheryl L.; McCracken, George H.; Hansen, Eric J.

    1998-01-01

    The UspA surface antigen of Moraxella catarrhalis was recently shown to be comprised of two different proteins (UspA1 and UspA2) which share an internal region containing 140 amino acids with 93% identity (C. Aebi, I. Maciver, J. L. Latimer, L. D. Cope, M. K. Stevens, S. E. Thomas, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 65:4367–4377, 1997). Isogenic uspA1, uspA2, and uspA1 uspA2 mutants were tested in a number of in vitro systems to determine what effect these mutations, either individually or together, might exert on the phenotype of M. catarrhalis 035E. Monoclonal antibodies specific for UspA1 or UspA2 were used in an indirect antibody accessibility assay to prove that both of these proteins were expressed on the surface of M. catarrhalis. All three mutants grew in vitro at the same rate and did not exhibit autoagglutination or hemagglutination properties that were detectably different from those of the wild-type parent strain. When tested for the ability to adhere to human epithelial cells, the wild-type parent strain and the uspA2 mutant readily attached to Chang conjunctival cells. In contrast, the uspA1 mutant and the uspA1 uspA2 double mutant both attached to these epithelial cells at a level nearly 2 orders of magnitude lower than that obtained with the wild-type parent strain, a result which suggested that expression of UspA1 by M. catarrhalis is essential for attachment to these epithelial cells. Both the wild-type parent strain and the uspA1 mutant were resistant to the bactericidal activity of normal human serum, whereas the uspA2 mutant and the uspA1 uspA2 double mutant were readily killed by this serum. This latter result indicated that the presence of UspA2 is essential for expression of serum resistance by M. catarrhalis. PMID:9632574

  15. Expression of the Moraxella catarrhalis UspA1 Protein Undergoes Phase Variation and Is Regulated at the Transcriptional Level

    PubMed Central

    Lafontaine, Eric R.; Wagner, Nikki J.; Hansen, Eric J.

    2001-01-01

    The UspA1 protein of Moraxella catarrhalis has been shown to function as an adhesin that mediates adherence to human epithelial cell lines in vitro (E. R. Lafontaine, L. D. Cope, C. Aebi, J. L. Latimer, G. H. McCracken, Jr., and E. J. Hansen, J. Bacteriol. 182:1364–1373, 2000). In the present study, cell lysates prepared from individual colonies of several M. catarrhalis wild-type strains were analyzed by Western blot analysis using monoclonal antibodies (MAbs) specific for the UspA1 protein. Expression of UspA1 was shown to exhibit phase variation that was correlated with both adherence ability in vitro and the number of guanine (G) residues contained within a homopolymeric [poly(G)]tract located upstream of the uspA1 open reading frame (ORF). Nucleotide sequence analysis revealed that isolates expressing relatively high levels of UspA1 had 10 G residues in their uspA1 poly(G)tracts, whereas isolates that expressed much lower levels of UspA1 had 9 G residues. This poly(G) tract was located 30 nucleotides (nt) upstream of the uspA1 ORF and 168 nt downstream of the uspA1 transcriptional start site. Primer extension experiments, RNA slot blot analysis, and cat reporter constructs were used to demonstrate that M. catarrhalis isolates with 10 G residues in their uspA1 poly(G) tracts expressed two-to threefold more uspA1 mRNA than did isolates which had 9 G residues in their poly(G)tracts. Northern hybridization analysis revealed that an intact uspA1 mRNA was readily detectable in RNA from M. catarrhalis isolates that had 10 G residues in their uspA1 poly(G) tracts, whereas no full-length uspA1 mRNA was observed in isolates whose poly(G)tracts contained 9 G residues. M. catarrhalis strain O35E uspA1 genes that contained wild-type and mutated poly(G) tracts were expressed in Haemophilus influenzae to demonstrate that the length and composition of the poly(G)tract affected expression of UspA1. PMID:11160084

  16. Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035E.

    PubMed

    Aebi, C; Lafontaine, E R; Cope, L D; Latimer, J L; Lumbley, S L; McCracken, G H; Hansen, E J

    1998-07-01

    The UspA surface antigen of Moraxella catarrhalis was recently shown to be comprised of two different proteins (UspA1 and UspA2) which share an internal region containing 140 amino acids with 93% identity (C. Aebi, I. Maciver, J. L. Latimer, L. D. Cope, M. K. Stevens, S. E. Thomas, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 65:4367-4377, 1997). Isogenic uspA1, uspA2, and uspA1 uspA2 mutants were tested in a number of in vitro systems to determine what effect these mutations, either individually or together, might exert on the phenotype of M. catarrhalis 035E. Monoclonal antibodies specific for UspA1 or UspA2 were used in an indirect antibody accessibility assay to prove that both of these proteins were expressed on the surface of M. catarrhalis. All three mutants grew in vitro at the same rate and did not exhibit autoagglutination or hemagglutination properties that were detectably different from those of the wild-type parent strain. When tested for the ability to adhere to human epithelial cells, the wild-type parent strain and the uspA2 mutant readily attached to Chang conjunctival cells. In contrast, the uspA1 mutant and the uspA1 uspA2 double mutant both attached to these epithelial cells at a level nearly 2 orders of magnitude lower than that obtained with the wild-type parent strain, a result which suggested that expression of UspA1 by M. catarrhalis is essential for attachment to these epithelial cells. Both the wild-type parent strain and the uspA1 mutant were resistant to the bactericidal activity of normal human serum, whereas the uspA2 mutant and the uspA1 uspA2 double mutant were readily killed by this serum. This latter result indicated that the presence of UspA2 is essential for expression of serum resistance by M. catarrhalis. PMID:9632574

  17. Multicentre in-vitro evaluation of the susceptibility of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis to ciprofloxacin, clarithromycin, co-amoxiclav and sparfloxacin.

    PubMed

    Hoogkamp-Korstanje, J A; Dirks-Go, S I; Kabel, P; Manson, W L; Stobberingh, E E; Vreede, R W; Davies, B I

    1997-03-01

    Seven laboratories, including a reference laboratory, tested the susceptibility of Moraxella catarrhalis, Streptococcus pneumoniae and Haemophilus influenzae strains to ciprofloxacin, clarithromycin, co-amoxiclav and sparfloxacin with the Etest. A total of 976 strains were collected. The results with ciprofloxacin and sparfloxacin were consistent for all laboratories, while those with clarithromycin and co-amoxiclav were not. The agreement between Etest MICs and broth microdilution was: ciprofloxacin and sparfloxacin, >95%; clarithromycin for all species, 71-85%; co-amoxiclav for H. influenzae, 31%. MIC90 values (broth dilution, mg/L) for M. catarrhalis, S. pneumoniae and H. influenzae were: sparfloxacin, 0.06, 0.5, 0.03; ciprofloxacin, 0.12, 2.0, 0.03; co-amoxiclav, 0.25, 0.25, 0.25; clarithromycin 0.25, 0.25 and 16. PMID:9096192

  18. A Moraxella catarrhalis two-component signal transduction system necessary for growth in liquid media affects production of two lysozyme inhibitors.

    PubMed

    Joslin, Stephanie N; Pybus, Christine; Labandeira-Rey, Maria; Evans, Amanda S; Attia, Ahmed S; Brautigam, Chad A; Hansen, Eric J

    2015-01-01

    There are a paucity of data concerning gene products that could contribute to the ability of Moraxella catarrhalis to colonize the human nasopharynx. Inactivation of a gene (mesR) encoding a predicted response regulator of a two-component signal transduction system in M. catarrhalis yielded a mutant unable to grow in liquid media. This mesR mutant also exhibited increased sensitivity to certain stressors, including polymyxin B, SDS, and hydrogen peroxide. Inactivation of the gene (mesS) encoding the predicted cognate sensor (histidine) kinase yielded a mutant with the same inability to grow in liquid media as the mesR mutant. DNA microarray and real-time reverse transcriptase PCR analyses indicated that several genes previously shown to be involved in the ability of M. catarrhalis to persist in the chinchilla nasopharynx were upregulated in the mesR mutant. Two other open reading frames upregulated in the mesR mutant were shown to encode small proteins (LipA and LipB) that had amino acid sequence homology to bacterial adhesins and structural homology to bacterial lysozyme inhibitors. Inactivation of both lipA and lipB did not affect the ability of M. catarrhalis O35E to attach to a human bronchial epithelial cell line in vitro. Purified recombinant LipA and LipB fusion proteins were each shown to inhibit human lysozyme activity in vitro and in saliva. A lipA lipB deletion mutant was more sensitive than the wild-type parent strain to killing by human lysozyme in the presence of human apolactoferrin. This is the first report of the production of lysozyme inhibitors by M. catarrhalis. PMID:25312959

  19. Moraxella catarrhalis-produced nitric oxide has dual roles in pathogenicity and clearance of infection in bacterial-host cell co-cultures.

    PubMed

    Mocca, Brian; Yin, Dandan; Gao, Yamei; Wang, Wei

    2015-12-01

    In humans, the free radical nitric oxide (NO) is a concentration-dependent multifunctional signaling or toxic molecule that modulates various physiological and pathological processes, and innate immunity against bacterial infections. Because the expression of bacterial genes encoding nitrite reductase (AniA) and NO reductase (NorB) is highly upregulated in biofilms in vitro, it is important to investigate whether bacterial NO-metabolism might subvert host NO signaling and play pathogenic roles during infection. The Moraxella catarrhalis AniA and NorB directly function in production and reduction of NO. Using M. catarrhalis-human bronchial epithelial cell (HBEC) co-cultures, we recently reported AniA/nitrite-dependent cytotoxic effects on HBECs, including altered protein profiles of HBECs and induced HBEC apoptosis, suggesting bacterial nitrite reduction likely dysregulates host cell gene expression. To further clarify whether nitrite reduction-derived NO or nitrite-dependent stimulation of bacterial growth was responsible for adverse effects on HBECs, we monitored bacterial nitrite reduction, levels of NO in co-cultures and resulted dynamic effects on HBEC proliferation and bacterial viability. This study demonstrated that M. catarrhalis nitrite reduction-derived NO was responsible for observed adverse effects on HBECs at mid-to-late stages of infection. More importantly, our data showed that while nitrite promoted bacterial growth and biofilm formation at early hours of infection, nitrite reduction-derived NO was toxic towards M. catarrhalis in maturing biofilms, suggesting nitrite reduction-derived NO might be a possible dualistic mechanism by which M. catarrhalis promotes diseases and spontaneous resolutions. PMID:26537639

  20. The UspA1 Protein and a Second Type of UspA2 Protein Mediate Adherence of Moraxella catarrhalis to Human Epithelial Cells In Vitro

    PubMed Central

    Lafontaine, Eric R.; Cope, Leslie D.; Aebi, Christoph; Latimer, Jo L.; McCracken, George H.; Hansen, Eric J.

    2000-01-01

    The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicated that UspA1 was involved in adherence of this organism to Chang conjunctival epithelial cells in vitro and that expression of UspA2 was essential for resistance of this strain to killing by normal human serum (C. Aebi, E. R. Lafontaine, L. D. Cope, J. L. Latimer, S. R. Lumbley, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 66:3113–3119, 1998). In the present study, isogenic uspA1, uspA2, and uspA1 uspA2 mutations were constructed in three additional M. catarrhalis strains: 012E, TTA37, and 046E. The uspA1 mutant of strain 012E had a decreased ability to attach to Chang cells. However, inactivation of the uspA1 gene in both strain TTA37 and strain 046E did not cause a significant decrease in attachment ability. Inactivation of the uspA2 gene of strain TTA37 did result in a loss of attachment ability. Nucleotide sequence analysis revealed that the predicted protein encoded by the uspA2 genes of both strains TTA37 and 046E had a N-terminal half that resembled the N-terminal half of UspA1 proteins, whereas the C-terminal half of this protein was nearly identical to those of previously characterized UspA2 proteins. The gene encoding this “hybrid” protein was designated uspA2H. PCR-based analysis revealed that approximately 20% of M. catarrhalis strains apparently possess a uspA2H gene instead of a uspA2 gene. The M. catarrhalis uspA1, uspA2, and uspA2H genes were cloned and expressed in Haemophilus influenzae cells, which were used to prove that both the UspA1 and UspA2H proteins can function as adhesins in vitro. PMID:10671460

  1. The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro.

    PubMed

    Lafontaine, E R; Cope, L D; Aebi, C; Latimer, J L; McCracken, G H; Hansen, E J

    2000-03-01

    The UspA1 and UspA2 proteins of Moraxella catarrhalis are structurally related, are exposed on the bacterial cell surface, and migrate as very high-molecular-weight complexes in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Previous analysis of uspA1 and uspA2 mutants of M. catarrhalis strain 035E indicated that UspA1 was involved in adherence of this organism to Chang conjunctival epithelial cells in vitro and that expression of UspA2 was essential for resistance of this strain to killing by normal human serum (C. Aebi, E. R. Lafontaine, L. D. Cope, J. L. Latimer, S. R. Lumbley, G. H. McCracken, Jr., and E. J. Hansen, Infect. Immun. 66:3113-3119, 1998). In the present study, isogenic uspA1, uspA2, and uspA1 uspA2 mutations were constructed in three additional M. catarrhalis strains: 012E, TTA37, and 046E. The uspA1 mutant of strain 012E had a decreased ability to attach to Chang cells. However, inactivation of the uspA1 gene in both strain TTA37 and strain 046E did not cause a significant decrease in attachment ability. Inactivation of the uspA2 gene of strain TTA37 did result in a loss of attachment ability. Nucleotide sequence analysis revealed that the predicted protein encoded by the uspA2 genes of both strains TTA37 and 046E had a N-terminal half that resembled the N-terminal half of UspA1 proteins, whereas the C-terminal half of this protein was nearly identical to those of previously characterized UspA2 proteins. The gene encoding this "hybrid" protein was designated uspA2H. PCR-based analysis revealed that approximately 20% of M. catarrhalis strains apparently possess a uspA2H gene instead of a uspA2 gene. The M. catarrhalis uspA1, uspA2, and uspA2H genes were cloned and expressed in Haemophilus influenzae cells, which were used to prove that both the UspA1 and UspA2H proteins can function as adhesins in vitro. PMID:10671460

  2. Detection of antibody responses against Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis proteins in children with community-acquired pneumonia: effects of combining pneumococcal antigens, pre-existing antibody levels, sampling interval, age, and duration of illness.

    PubMed

    Borges, I C; Andrade, D C; Vilas-Boas, A-L; Fontoura, M-S H; Laitinen, H; Ekström, N; Adrian, P V; Meinke, A; Cardoso, M-R A; Barral, A; Ruuskanen, O; Käyhty, H; Nascimento-Carvalho, C M

    2015-08-01

    We evaluated the effects of combining different numbers of pneumococcal antigens, pre-existing antibody levels, sampling interval, age, and duration of illness on the detection of IgG responses against eight Streptococcus pneumoniae proteins, three Haemophilus influenzae proteins, and five Moraxella catarrhalis proteins in 690 children aged <5 years with pneumonia. Serological tests were performed on acute and convalescent serum samples with a multiplexed bead-based immunoassay. The median sampling interval was 19 days, the median age was 26.7 months, and the median duration of illness was 5 days. The rate of antibody responses was 15.4 % for at least one pneumococcal antigen, 5.8 % for H. influenzae, and 2.3 % for M. catarrhalis. The rate of antibody responses against each pneumococcal antigen varied from 3.5 to 7.1 %. By multivariate analysis, pre-existing antibody levels showed a negative association with the detection of antibody responses against pneumococcal and H. influenzae antigens; the sampling interval was positively associated with the detection of antibody responses against pneumococcal and H. influenzae antigens. A sampling interval of 3 weeks was the optimal cut-off for the detection of antibody responses against pneumococcal and H. influenzae proteins. Duration of illness was negatively associated with antibody responses against PspA. Age did not influence antibody responses against the investigated antigens. In conclusion, serological assays using combinations of different pneumococcal proteins detect a higher rate of antibody responses against S. pneumoniae compared to assays using a single pneumococcal protein. Pre-existing antibody levels and sampling interval influence the detection of antibody responses against pneumococcal and H. influenzae proteins. These factors should be considered when determining pneumonia etiology by serological methods in children. PMID:25894988

  3. Ligand-bound structures of 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase from Moraxella catarrhalis reveal a water channel connecting to the active site for the second step of catalysis.

    PubMed

    Dhindwal, Sonali; Priyadarshini, Priyanka; Patil, Dipak N; Tapas, Satya; Kumar, Pramod; Tomar, Shailly; Kumar, Pravindra

    2015-02-01

    KdsC, the third enzyme of the 3-deoxy-D-manno-octulosonic acid (KDO) biosynthetic pathway, catalyzes a substrate-specific reaction to hydrolyze 3-deoxy-D-manno-octulosonate 8-phosphate to generate a molecule of KDO and phosphate. KdsC is a phosphatase that belongs to the C0 subfamily of the HAD superfamily. To understand the molecular basis for the substrate specificity of this tetrameric enzyme, the crystal structures of KdsC from Moraxella catarrhalis (Mc-KdsC) with several combinations of ligands, namely metal ion, citrate and products, were determined. Various transition states of the enzyme have been captured in these crystal forms. The ligand-free and ligand-bound crystal forms reveal that the binding of ligands does not cause any specific conformational changes in the active site. However, the electron-density maps clearly showed that the conformation of KDO as a substrate is different from the conformation adopted by KDO when it binds as a cleaved product. Furthermore, structural evidence for the existence of an intersubunit tunnel has been reported for the first time in the C0 subfamily of enzymes. A role for this tunnel in transferring water molecules from the interior of the tetrameric structure to the active-site cleft has been proposed. At the active site, water molecules are required for the formation of a water bridge that participates as a proton shuttle during the second step of the two-step phosphoryl-transfer reaction. In addition, as the KDO biosynthesis pathway is a potential antibacterial target, pharmacophore-based virtual screening was employed to identify inhibitor molecules for the Mc-KdsC enzyme. PMID:25664734

  4. Moraxella Catarrhalis: A Common Cause of Childhood Illnesses

    MedlinePLUS

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  5. [Spondylodiscitis due to Moraxella lacunata].

    PubMed

    Masse-Chabredier, I; Mizony, M-H

    2007-11-01

    Infectious spondylodiscitis is an uncommon disease, often diagnosed late. The main pathogen is Staphylococcus aureus. We report a case of spondylodiscitis diagnosed early, caused by Moraxella lacunata, a Gram-negative bacillus of usually low pathogenicity for man. A combination of amoxicillin 12 g/day IV then per os, and ciprofloxacine 1,500 mg/day per os was given for 8 weeks. Evolution was favorable. PMID:17336012

  6. Branhamella catarrhalis: an organism gaining respect as a pathogen.

    PubMed Central

    Catlin, B W

    1990-01-01

    Branhamella catarrhalis was formerly regarded as a common, essentially harmless inhabitant of the pharynx. This misapprehension was caused, in part, by confusion with another pharyngeal resident, Neisseria cinerea. The two organisms can now be differentiated by the positive reactions of B. catarrhalis in tests for nitrate reduction and hydrolysis of tributyrin and DNase. B. catarrhalis is currently recognized as the third most frequent cause of acute otitis media and acute sinusitis in young children. It often causes acute exacerbations of chronic bronchopulmonary disease in older or immunocompromised adults and is incriminated occasionally in meningitis, endocarditis, bacteremia, conjunctivitis, keratitis, and urogenital infections. Virulence-associated factors, such as pili, capsules, outer membrane vesicles, iron acquisition proteins, histamine-synthesizing ability, resistance to the bactericidal action of normal human serum, and binding to the C1q complement component, have been identified in some strains. beta-Lactamase producing strains, first detected in 1976, have risen to approximately 75% worldwide. Thus far, however, practically all American strains of B. catarrhalis remain susceptible to alternative antibiotics. A possible selective advantage of recent isolates is their reportedly heightened tendency for adherence to oropharyngeal cells from patients with chronic bronchopulmonary disease. Images PMID:2121328

  7. Phylogenetic analysis and genetic diversity of 3' region of rtxA gene from geographically diverse strains of Moraxella bovis, Moraxella bovoculi and Moraxella ovis.

    PubMed

    Farias, Luana D'Avila; Maboni, Grazieli; Matter, Letícia Beatriz; Scherer, Charles Fernando Capinos; Libardoni, Felipe; de Vargas, Agueda Castagna

    2015-08-01

    The cytotoxin A (MbxA) is one of the main virulence factors of Moraxella bovis involved in the pathogenesis of infectious bovine keratoconjunctivitis (IBK). Moraxella ovis and Moraxella bovoculi, suspected to be associated with infectious keratitis in sheep and cattle respectively, also have a gene that encodes the cytotoxin A (movA and mbvA, respectively). The aim of this study was to determine the molecular sequence of the 3' region of the cytotoxin gene of Moraxella spp. strains isolated from clinical cases to establish phylogenetic and evolutionary comparisons. PCR amplification, nucleotide sequencing (nt) and amino acid (aa) sequence prediction were performed, followed by the sequences comparison, identity level calculation and selective pressure analysis. The phylogenetic reconstruction based on nt and aa sequences clearly differentiate M. bovis (n=15), M. bovoculi (n=11) and M. ovis (n=7) and their respective reference strains. An alignment of 843nt revealed high similarity within bacterial species (MbxA=99.9% nt and aa; MbvA=99.3% nt and 98.8% aa; MovA=99.5% nt and 99.3% aa). The similarity of partial sequences (nt 1807-2649) of MbxA in relation to MbvA and MovA ranged from 76.3 to 78.5%; similarity between MbvA and MovA ranged from 95.7 to 97.5%. A negative selection on mbvA and movA sequences was revealed by the molecular evolution analysis. The phylogenetic analysis of movA and mbvA allowed different strains of Moraxella spp. to be grouped according to the period of isolation. Sequence analysis of cytotoxin may provide insights into genetic and evolutionary relationships and into the genetic/molecular basis of Moraxella spp. PMID:26036790

  8. Differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovoculi and M. ovis

    PubMed Central

    Maboni, Grazieli; Gressler, Leticia T.; Espindola, Julia P.; Schwab, Marcelo; Tasca, Caiane; Potter, Luciana; de Vargas, Agueda Castagna

    2015-01-01

    The aim of this study was to determine the differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovoculi and M. ovis. Thirty-two strains of Moraxella spp. isolated from cattle and sheep with infectious keratoconjunctivitis were tested via broth microdilution method to determine their susceptibility to ampicillin, cefoperazone, ceftiofur, cloxacillin, enrofloxacin, florfenicol, gentamicin, neomycin, oxytetracycline and penicillin. The results demonstrated that Moraxella spp. strains could be considered sensitive for most of the antimicrobials tested in this study, but differences between the antimicrobial susceptibility profiles of these three Moraxella species were found. M. bovis might differ from other species due to the higher MIC and MBC values it presented. PMID:26273272

  9. Differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovoculi and M. ovis.

    PubMed

    Maboni, Grazieli; Gressler, Leticia T; Espindola, Julia P; Schwab, Marcelo; Tasca, Caiane; Potter, Luciana; de Vargas, Agueda Castagna

    2015-06-01

    The aim of this study was to determine the differences in the antimicrobial susceptibility profiles of Moraxella bovis, M. bovoculi and M. ovis. Thirty-two strains of Moraxella spp. isolated from cattle and sheep with infectious keratoconjunctivitis were tested via broth microdilution method to determine their susceptibility to ampicillin, cefoperazone, ceftiofur, cloxacillin, enrofloxacin, florfenicol, gentamicin, neomycin, oxytetracycline and penicillin. The results demonstrated that Moraxella spp. strains could be considered sensitive for most of the antimicrobials tested in this study, but differences between the antimicrobial susceptibility profiles of these three Moraxella species were found. M. bovis might differ from other species due to the higher MIC and MBC values it presented. PMID:26273272

  10. Antigenic analysis of fimbrial proteins from Moraxella bovis.

    PubMed Central

    Moore, L J; Rutter, J M

    1987-01-01

    Fimbrial proteins were extracted from 15 isolates of Moraxella bovis, and antisera to each of the preparations were raised in rabbits. The antigenic relationships of the fimbriae were investigated by an enzyme-linked immunosorbent assay, tandem crossed immunoelectrophoresis, and a slide agglutination test. With all three methods there was a similar pattern of antigenic cross-reactivity among the fimbriae. The 15 isolates, together with 23 additional isolates, could be grouped into seven fimbrial serogroups. Images PMID:2891723

  11. Moraxella Species Are Primarily Responsible for Generating Malodor in Laundry

    PubMed Central

    Mitani, Asako; Niwano, Yu; Takeuchi, Kohei; Tanaka, Atsushi; Yamaguchi, Noriko; Kawamura, Yoshiaki; Hitomi, Jun

    2012-01-01

    Many people in Japan often detect an unpleasant odor generated from laundry that is hung to dry indoors or when using their already-dried laundry. Such an odor is often described as a “wet-and-dirty-dustcloth-like malodor” or an “acidic or sweaty odor.” In this study, we isolated the major microorganisms associated with such a malodor, the major component of which has been identified as 4-methyl-3-hexenoic acid (4M3H). The isolates were identified as Moraxella osloensis by morphological observation and biochemical and phylogenetic tree analyses. M. osloensis has the potential to generate 4M3H in laundry. The bacterium is known to cause opportunistic infections but has never been known to generate a malodor in clothes. We found that M. osloensis exists at a high frequency in various living environments, particularly in laundry in Japan. The bacterium showed a high tolerance to desiccation and UV light irradiation, providing one of the possible reasons why they survive in laundry during and even after drying. PMID:22367080

  12. The metabolism of benzoate by Moraxella species through anaerobic nitrate respiration. Evidence for a reductive pathway.

    PubMed

    Williams, R J; Evans, W C

    1975-04-01

    Moraxella sp. isolated from soil grows anaerobically on benzoate by nitrate respiration; nitrate or nitrite are obligatory electron acceptors, being reduced to molecular N2 during the catabolism of the substrate. This bacterium also grows aerobically on benzoate. 2. Aerobically, benzoate is metabolized by ortho cleavage of catechol followed by the beta-oxoadipate pathway. 3. Cells of Moraxella grown anaerobically on benzoate are devoid of ortho and meta cleavage enzymes; cyclohexanecarboxylate and 2-hydroxycyclohexanecarboxylate were detected in the anaerobic culture fluid. 4. [ring-U-14C]Benzoate, incubated anaerobically with cells in nitrate-phosphate buffer, gave rise to labelled 2-hydroxycyclohexanecarboxylate and adipate. When [carboxy-14C]benzoate was used, 2-hydroxycyclohexanecarboxylate was radioactive but the adipate was not labelled. A decarboxylation reaction intervenes at some stage between these two metabolites. 5. The anaerobic metabolism of benzoate by Moraxella sp. through nitrate respiration takes place by the reductive pathway (Dutton & Evans, 1969). Hydrogenation of the aromatic ring probably occurs via cyclohexa-2,5-dienecarboxylate and cyclohex-1-enecarboxylate to give cyclohexanecarboxylate. The biochemistry of this reductive process remains unclear. 6. CoA thiol esterification of cyclohexanecarboxylate followed by beta-oxidation via the unsaturated and hydroxy esters, would afford 2-oxocyclohexanecarboxylate. Subsequent events in the Moraxella culture differ from those occurring with Rhodopseudomonas palustris; decarboxylation precedes hydrolytic cleavage of the alicyclic ring to produce adipate in the former, whereas in the latter the keto ester undergoes direct hydrolytic fission to pimelate. PMID:1156389

  13. Septic Arthritis Due to Moraxella osloensis in a Rhesus Macaque (Macaca mulatta)

    PubMed Central

    Wren, Melissa A; Caskey, John R; Liu, David X; Embers, Monica E

    2013-01-01

    A 5.5-y-old Chinese-origin female rhesus macaque (Macaca mulatta) presented for bilateral hindlimb lameness. The primate had been group-reared in an SPF breeding colony and was seronegative for Macacine herpesvirus 1, SIV, simian retrovirus type D, and simian T-lymphotropic virus. The macaque's previous medical history included multiple occasions of swelling in the left tarsus, and trauma to the right arm and bilateral hands. In addition, the macaque had experienced osteomyelitis of the left distal tibia and rupture of the right cranial cruciate ligament that had been surgically repaired. Abnormal physical examination findings on presentation included a thin body condition, mild dehydration, and bilaterally swollen stifles that were warm to the touch, with the right stifle more severely affected. Mild instability in the left stifle was noted, and decreased range of motion and muscle atrophy were present bilaterally. Hematologic findings included marked neutrophilia and lymphopenia and moderate anemia. Arthrocentesis and culture of joint fluid revealed Moraxella-like organisms. Treatment with enrofloxacin was initiated empirically and subsequently switched to cephalexin, which over time alleviated the joint swelling and inflammation. Definitive diagnosis of Moraxella osloensis septic arthritis was made through isolation of the organism and sequencing of the 16S rDNA region. To our knowledge, this report is the first description of Moraxella osloensis septic arthritis in a rhesus macaque. PMID:24326229

  14. A direct pathway for the metabolism of propionate in cell extracts from Moraxella lwoffi.

    PubMed

    Hodgson, B; McGarry, J D

    1968-03-01

    1. Extracts from Moraxella lwoffi oxidize propionate, but at a low rate when compared with whole cells. 2. This oxidative activity requires the formation of propionyl-CoA. 3. Enzymes catalysing the formation of propionyl phosphate and propionyl-CoA are present. The presence of a propionyl-CoA hydrolase is considered to be an artifact, but partly responsible for the low rates of oxidation. 4. Enzymes catalysing the reduction of NAD(+) and the formation of pyruvate with propionyl-CoA as substrate are also present. 5. That the only pathway for the metabolism of propionate in extracts is a direct one to acetate via pyruvate was confirmed by the use of (14)C-labelled materials. 6. A possible sequence of enzyme-catalysed reactions that will account for the experimental observations is described. PMID:5642619

  15. A direct pathway for the metabolism of propionate in cell extracts from Moraxella Iwoffi

    PubMed Central

    Hodgson, B.; McGarry, J. D.

    1968-01-01

    1. Extracts from Moraxella lwoffi oxidize propionate, but at a low rate when compared with whole cells. 2. This oxidative activity requires the formation of propionyl-CoA. 3. Enzymes catalysing the formation of propionyl phosphate and propionyl-CoA are present. The presence of a propionyl-CoA hydrolase is considered to be an artifact, but partly responsible for the low rates of oxidation. 4. Enzymes catalysing the reduction of NAD+ and the formation of pyruvate with propionyl-CoA as substrate are also present. 5. That the only pathway for the metabolism of propionate in extracts is a direct one to acetate via pyruvate was confirmed by the use of 14C-labelled materials. 6. A possible sequence of enzyme-catalysed reactions that will account for the experimental observations is described. PMID:5642619

  16. UV light-induced survival response in a highly radiation-resistant isolate of the Moraxella-acinetobacter group

    SciTech Connect

    Keller, L.C.; Thompson, T.L.; Maxcy, R.B.

    1982-02-01

    A highly radiation-resistant member of the Moraxella-Acinetobacter group, isolate 4, obtained from meat, was studied to determine the effect of preexposure to UV radiation on subsequent UV light resistance. Cultures that were preexposed to UV light and incubated for a short time in plate count broth exhibited increased survival of a UV light challenge dose. This response was inhibited in the presence of chloramphenicol. Frequencies of mutation to streptomycin, trimethoprim, and sulfanilamide resistance remained the same after the induction of this survival response and were not altered by treatment with mutagens, with the exception of mutation to streptomycin resistance after ..gamma..-irradiation or nitrosoguanidine or methyl methane sulfonate treatment. The results indicated that isolate 4 has a UV light-inducible UV light resistance mechanism which is not associated with increased mutagenesis. The characteristics of the radiation resistance response in this organism are similar to those of certain other common food contaminants. Therefore, considered as part of the total microflora of meat, isolate 4 and the other radiation-resistant Moraxella-Acinetobacter isolates should not pose unique problems in a proposed radappertizaton process.

  17. Development of the radiation-resistant strain of Moraxella osloensis and effect of penicillin G on its growth

    NASA Astrophysics Data System (ADS)

    Lim, Sangyong; Yun, Hyejeong; Joe, Minho; Kim, Dongho

    2009-07-01

    A series of repeated exposures to ?-radiation with intervening outgrowth of survivors was used to develop radioresistant cultures of Moraxella osloensis that have been recognized as potential pathogenic microorganism. The D10 value of the radiation-resistant strain, 5.903±0.006 kGy, was increased by four-fold compared to the parent wild-type strain, 1.637±0.004 kGy. Since most strains of M. osloensis are sensitive to penicillin, we have surveyed the sensitivity of radiation-resistant strain to this antibiotic. When the optical density was monitored after the addition of penicillin G, the radioresistant strain appeared to be more resistant to only a low concentration of penicillin G (0.5 U/ml) than the parent strain. Interestingly, however, there was no apparent difference in the number of viable cells between both strains. Scanning electron microscope data showed that the resistance cells were generally larger than the parent cells, suggesting that this increase in size may cause a higher optical density of radioresistant cells. In conclusion, radiation mutation does not affect the penicillin resistance of M. osloensis.

  18. Fermentation Products of Solvent Tolerant Marine Bacterium Moraxella spp. MB1 and Its Biotechnological Applications in Salicylic Acid Bioconversion

    PubMed Central

    Wahidullah, Solimabi; Naik, Deepak N.; Devi, Prabha

    2013-01-01

    As part of a proactive approach to environmental protection, emerging issues with potential impact on the environment is the subject of ongoing investigation. One emerging area of environmental research concerns pharmaceuticals like salicylic acid, which is the main metabolite of various analgesics including aspirin. It is a common component of sewage effluent and also an intermediate in the degradation pathway of various aromatic compounds which are introduced in the marine environment as pollutants. In this study, biotransformation products of salicylic acid by seaweed, Bryopsis plumosa, associated marine bacterium, Moraxella spp. MB1, have been investigated. Phenol, conjugates of phenol and hydroxy cinnamic acid derivatives (coumaroyl, caffeoyl, feruloyl and trihydroxy cinnamyl) with salicylic acid (3–8) were identified as the bioconversion products by electrospray ionization mass spectrometry. These results show that the microorganism do not degrade phenolic acid but catalyses oxygen dependent transformations without ring cleavage. The degradation of salicylic acid is known to proceed either via gentisic acid pathway or catechol pathway but this is the first report of biotransformation of salicylic acid into cinnamates, without ring cleavage. Besides cinnamic acid derivatives (9–12), metabolites produced by the bacterium include antimicrobial indole (13) and ?-carbolines, norharman (14), harman (15) and methyl derivative (16), which are beneficial to the host and the environment. PMID:24391802

  19. Ocular Immune Responses in Steers following Intranasal Vaccination with Recombinant Moraxella bovis Cytotoxin Adjuvanted with Polyacrylic Acid

    PubMed Central

    Edman, Judy M.; Chigerwe, Munashe

    2014-01-01

    Infectious bovine keratoconjunctivitis (IBK) caused by Moraxella bovis is the most common eye disease of cattle. The pathogenesis of M. bovis requires the expression of pili that enable the organism to attach to the ocular surface and an RTX (repeats in the structural toxin) toxin (cytotoxin or hemolysin), which is cytotoxic to corneal epithelial cells. In this pilot study, ocular mucosal immune responses of steers were measured following intranasal (i.n.) vaccination with a recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid. Beef steers were vaccinated with either 500 ?g (n = 3) or 200 ?g (n = 3) of recombinant M. bovis cytotoxin plus adjuvant. Control group steers (n = 2) were vaccinated with adjuvant alone, and all steers were given a booster on day 21. Antigen-specific tear IgA and tear IgG, tear cytotoxin-neutralizing antibody responses, and serum cytotoxin-neutralizing antibody responses were determined in samples collected prevaccination and on days 14, 28, 42, and 55. Changes in tear antigen-specific IgA levels from day 0 to days 28, 42, and 55 were significantly different between groups; however, in post hoc comparisons between individual group pairs at the tested time points, the differences were not significant. Our results suggest that i.n. vaccination of cattle with recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid effects changes in ocular antigen-specific IgA concentrations. The use of intranasally administered recombinant M. bovis cytotoxin adjuvanted with polyacrylic acid could provide an alternative to parenteral vaccination of cattle for immunoprophylaxis against IBK. PMID:24334685

  20. Assessment of Bdellovibrio bacteriovorus 109J killing of Moraxella bovis in an in vitro model of infectious bovine keratoconjunctivitis

    PubMed Central

    Boileau, Mélanie J.; Clinkenbeard, Kenneth D.; Iandolo, John J.

    2011-01-01

    The objective of this study was to determine the potential of Bdellovibrio bacteriovorus 109J as an alternative non-chemotherapeutic treatment of infectious bovine keratoconjunctivitis (IBK). To accomplish this, various parameters of B. bacteriovorus predation of Moraxella bovis were determined in vitro. Initial passage of B. bacteriovorus using M. bovis as prey required 10 d for active cultures to develop compared with 2 d for culture on normal Escherichia coli prey; however by the 5th passage, time to active predatory morphology was reduced to 2 d. This high passage B. bacteriovorus culture [1 × 1010 plaque forming units (PFU)/mL] killed 76% of M. bovis [1 × 107 colony forming units (CFU)/mL] present in suspension broth in a 4 h assay. The minimal level of M. bovis supporting B. bacteriovorus predation was 1 × 104 CFU/mL. To assess the ability of B. bacteriovorus to kill M. bovis on an epithelial surface mimicking IBK, an in vitro assay with Madin-Darby bovine kidney (MDBK) cells inoculated with 4 × 107 CFU/mL M. bovis was used. Treatment with a B. bacteriovorus suspension (1.6 × 1011 PFU/mL) decreased adherence of M. bovis to MDBK cells by 6-fold at 12 h of treatment, as well as decreased the number of unattached M. bovis cells by 1.4-fold. This study demonstrates that B. bacteriovorus has potential as an effective biological control of M. bovis at levels likely present in IBK-infected corneal epithelia and ocular secretions. PMID:22468026

  1. Haemophilus influenzae isolates survive for up to 20years at -70°C in skim milk tryptone glucose glycerol broth (STGGB) if thawing is avoided during re-culture.

    PubMed

    Hare, K M; Smith-Vaughan, H C; Beissbarth, J; Leach, A J

    2015-12-01

    Haemophilus influenzae remains a major cause of disease worldwide requiring continued study. Recently, isolates of Streptococcus pneumoniae and Moraxella catarrhalis, but not H. influenzae, were reported to survive long-term ultra-freeze storage in STGGB. We show that nontypeable H. influenzae isolates survive for up to 20years when thawing is avoided. PMID:26477641

  2. Vaccine 29 (2011) 16281633 Contents lists available at ScienceDirect

    E-print Network

    Boppart, Stephen

    2011-01-01

    causative agents of OM and their fre- quencies of involvement are Streptococcus pneumonia (35­60%), Haemophilus influenza (12­23%), and Moraxella catarrhalis (5­15%) [7,15­17]. Streptococcus pneumonia tissues [18]. S. pneumonia is a major gram positive human pathogen and the leading cause of pneumonia

  3. Distribution of pathogens causing nosocomial infection in patients with bronchial asthma.

    PubMed

    Mao, W; Cui, E H

    2015-01-01

    This study aimed to analyze nosocomial respiratory infection (NRI) in patients with bronchial asthma. Among the clinical data of 575 asthmatic patients that was collected and analyzed, 52 were diagnosed with NRI. The most common gram-positive bacterial species was Streptococcus pneumoniae, which was detected in 8 patients, whereas the predominant Gram-negative bacteria included Haemophilus influenzae (11 patients), Moraxella catarrhalis (8 patients), and Escherichia coli (7 patients). The simultaneous detection of all strains was predominant in patients older than 65 years of age, whereas the detection rates of S. pneumoniae, H. influenzae, E. coli, and M. catarrhalis were predominant in patients younger than 65 years old. The differences in the detection rates were not significant between the male and female groups. From this study, we can conclude that S. pneumoniae, H. influenzae, E. coli, and M. catarrhalis are common NRI-causing pathogens, and bacterial infection is the main risk factor for NRI in asthmatic patients. PMID:26662406

  4. Portable exhausters POR-004 SKID B, POR-005 SKID C, POR-006 SKID D storage plan

    SciTech Connect

    Nelson, O.D.

    1997-09-04

    This document provides a storage plan for portable exhausters POR-004 SKID B, POR-005 SKID C, AND POR-006 SKID D. The exhausters will be stored until they are needed by the TWRS (Tank Waste Remediation Systems) Saltwell Pumping Program. The storage plan provides criteria for portable exhauster storage, periodic inspections during storage, and retrieval from storage.

  5. One third of middle ear effusions from children undergoing tympanostomy tube placement had multiple bacterial pathogens

    PubMed Central

    2012-01-01

    Background Because previous studies have indicated that otitis media may be a polymicrobial disease, we prospectively analyzed middle ear effusions of children undergoing tympanostomy tube placement with multiplex polymerase chain reaction for four otopathogens. Methods Middle ear effusions from 207 children undergoing routine tympanostomy tube placement were collected and were classified by the surgeon as acute otitis media (AOM) for purulent effusions and as otitis media with effusion (OME) for non-purulent effusions. DNA was isolated from these samples and analyzed with multiplex polymerase chain reaction for Haemophilus influenzae, Streptococcus pneumoniae, Alloiococcus otitidis, and Moraxella catarrhalis. Results 119 (57%) of 207 patients were PCR positive for at least one of these four organisms. 36 (30%) of the positive samples indicated the presence of more than one bacterial species. Patient samples were further separated into 2 groups based on clinical presentation at the time of surgery. Samples were categorized as acute otitis media (AOM) if pus was observed behind the tympanic membrane. If no pus was present, samples were categorized as otitis media with effusion (OME). Bacteria were identified in most of the children with AOM (87%) and half the children with OME (51%, p?Moraxella catarrhalis were more frequently identified in middle ear effusions than Streptococcus pneumoniae. Conclusions Haemophilus influenzae, Streptococcus pneumoniae, Alloiococcus otitidis, and Moraxella catarrhalis were identified in the middle ear effusions of some patients with otitis media. Overall, we found AOM is predominantly a single organism infection and most commonly from Haemophilus influenzae. In contrast, OME infections had a more equal distribution of single organisms, polymicrobial entities, and non-bacterial agents. PMID:22741759

  6. Evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting Gram-negative rods.

    PubMed

    Bombicino, Karina A; Almuzara, Marisa N; Famiglietti, Angela M R; Vay, Carlos

    2007-01-01

    To evaluate the activity of pyrrolidonyl arylamidase (PYR) for the differentiation and identification of nonfermenting gram negative rods (NFGNR), 293 isolates were tested. A 24 h culture of each test organism was prepared. From this a 108-109 cfu/mL suspension was added to 0.25 mL of sterile physiologic solution. A PYR disk was then added and the test was incubated for 30 minutes at 35-37 degrees C, at environmental atmosphere. Reading was done by adding 1 drop of cinnamaldehyde reagent. Strains of Acinetobacter baumannii, Acinetobacter haemolyticus, Alcaligenes faecalis, Bergeyella zoohelcum, Bordetella bronchiseptica, Bordetella hinzii, Brevundimonas diminuta, Brevundimonas vesicularis, Brucella ovis, Brucella spp., Brucella suis, Burkholderia cepacia complex, Moraxella catarrhalis, Moraxella lacunata, Moraxella nonliquefaciens, Moraxella osloensis, Oligella ureolytica, Pseudomonas alcaligenes, Pseudomonas mendocina, Pseudomonas pseudoalcaligenes, Pseudomonas putida, Pseudomonas stutzeri, Pseudomonas Vb3, Psychrobacter phenylpyruvicus, and Stenotrophomonas maltophilia were PYR negative. On the other hand Achromobacter piechaudii, Achromobacter denitrificans, Achromobacter xylosoxidans, Burkholderia gladioli, Chryseobacterium gleum-indologenes, Comamonas testosroni, Cupriavidus pauculus, Delftia acidovorans, Elizabethkingia meningoseptica, Myroides spp., Ochrobactrum anthropi, Pseudomonas oryzihabitans, Ralstonia pickettii, Rhizobium radiobacter, Shewanella spp., Sphingobacterium multivorum, Sphingobacterium spiritivorum, and Weeksella virosa were PYR positive. Finally, Acinetobacter lwoffii, Pseudomonas aeruginosa, Pseudomonas fluorescens, Roseomonas spp., and Sphingomonas paucimobilis-parapaucimobilis were PYR variable. PYR testing should be considered as a useful tool to facilitate the identification of NFGNR. PMID:16822636

  7. ARQUITECTURA opta por la arquitectura

    E-print Network

    Hill, Wendell T.

    ARQUITECTURA PAISAJISTA ¡opta por la arquitectura paisajista! A Career with An Exciting Future, fotografía de Tom Fox. 636 Eye Street, NW, Washington, DC 20001 www.asla.org #12;¿Qué es la arquitectura personas? La arquitectura paisajista ofrece un interesante futuro y un gran potencial en cuanto a ingresos

  8. Cmo presentar una queja por discriminacin

    E-print Network

    Sheridan, Jennifer

    actividades de la Universidad, incluyendo la discriminación por sexo y el acoso sexual. Estas prohibiciones requerimientos del Título IX cubren la discriminación por sexo, el acoso sexual, la conducta sexual inapropiada y

  9. Centros oncológicos designados por el NCI

    Cancer.gov

    El programa de centros oncológicos designados por el Instituto Nacional del Cáncer (NCI) reconoce a los centros de todo el país que cumplen con rigurosos criterios para participar en proyectos avanzados de primer nivel para la investigación multidisciplinaria del cáncer.

  10. Consequences of POR mutations and polymorphisms

    PubMed Central

    Miller, Walter L.; Agrawal, Vishal; Sandee, Duanpen; Tee, Meng Kian; Huang, Ningwu; Choi, Ji Ha; Morrissey, Kari; Giacomini, Kathleen M.

    2015-01-01

    P450 oxidoreductase (POR) transports electrons from NADPH to all microsomal cytochrome P450 enzymes, including steroidogenic P450c17, P450c21 and P450aro. Severe POR mutations A287P (in Europeans) and R457H (in Japanese) cause the Antley-Bixler skeletal malformation syndrome (ABS) plus impaired steroidogenesis (causing genital anomalies), but the basis of ABS is unclear. We have characterized the activities of ~40 POR variants, showing that assays based on P450c17 activities, but not cytochrome c assays, correlate with the clinical phenotype. The human POR gene is highly polymorphic: the A503V sequence variant, which decreases P450c17 activities to ~60%, is found on ~28% of human alleles. A promoter polymorphism (~8% of Asians and ~13% of Caucasians) at ?152 reduces transcriptional activity by half. Screening of 35 POR variants showed that most mutants lacking activity with P450c17 or cytochrome c also lacked activity to support CYP1A2 and CYP2C19 metabolism of EOMCC (a fluorogenic non-drug substrate), although there were some remarkable differences: Q153R causes ABS and has ~30% of wild-type activity with P450c17 but had 144% of WT activity with CYP1A2 and 284% with CYP2C19. The effects of POR variants on CYP3A4, which metabolizes nearly 50% of clinically used drugs, was examined with multiple, clinically-relevant drug substrates, showing that A287P and R457H dramatically reduce drug metabolism, and that A503V variably impairs drug metabolism. The degree of activity can vary with the drug substrate assayed, as the drugs can influence the conformation of the P450. POR is probably an important contributor to genetic variation in both steroidogenesis and drug metabolism. PMID:21070833

  11. Volatile organic compounds generated by cultures of bacteria and viruses associated with respiratory infections.

    PubMed

    Abd El Qader, Amir; Lieberman, David; Shemer Avni, Yonat; Svobodin, Natali; Lazarovitch, Tsilia; Sagi, Orli; Zeiri, Yehuda

    2015-12-01

    Respiratory infections (RI) can be viral or bacterial in origin. In either case, the invasion of the pathogen results in production and release of various volatile organic compounds (VOCs). The present study examines the VOCs released from cultures of five viruses (influenza A, influenza B, adenovirus, respiratory syncitial virus and parainfluenza 1 virus), three bacteria (Moraxella catarrhalis, Haemophilus influenzae and Legionella pneumophila) and Mycoplasma pneumoniae isolated colonies. Our results demonstrate the involvement of inflammation-induced VOCs. Two significant VOCs were identified as associated with infectious bacterial activity, heptane and methylcyclohexane. These two VOCs have been linked in previous studies to oxidative stress effects. In order to distinguish between bacterial and viral positive cultures, we performed principal component analysis including peak identity (retention time) and VOC concentration (i.e. area under the peak) revealing 1-hexanol and 1-heptadecene to be good predictors. Copyright © 2015 John Wiley & Sons, Ltd. PMID:26033043

  12. Photodynamic therapy with water-soluble phtalocyanines against bacterial biofilms in teeth root canals

    NASA Astrophysics Data System (ADS)

    Gergova, Raina; Georgieva, Tzvetelina; Angelov, Ivan; Mantareva, Vanya; Valkanov, Serjoga; Mitov, Ivan; Dimitrov, Slavcho

    2012-06-01

    The study presents the PDT with metal phthalocyanines on biofilms grown in root canals of ten representatives of the Gram-positive and the Gram-negative bacterial species and a fungus Candida albicans which cause aqute teeth infections in root canals.. The extracted human single-root teeth infected for 48 h with microorganisms in conditions to form biofilms of the above pathogens were PDT treated. The stage of biofilm formation and PDT effect of the samples of the teeth were determined by the scaning electron microscopy and with standard microbial tests. The PDT treating procedure included 10 min incubation with the respected phthalocyanine and irradiated with 660 nm Diode laser for 10 min. The most strongly antibacterial activity was achieved with zinc(II) phthalocyanine (ZnPc) against Enterococcus faecalis, Staphylococcus aureus and Moraxella catarrhalis. The other Gram-negative bacteria and Candida albicans were 10-100 times more resistant than the Gram-positive species. The Gram-negative Moraxella catarrhalis and Acinetobacter baumannii were more sensitive than the enterobacteria, but eradication of Pseudomonas aeruginosa in biofilm was insignificant. The influence of the stage of biofilm formation and the initial conditions (bacterial density, photosensitizer concentration and energy fluence of radiation) to the obtained level of inactivation of biofilms was investigated. The PDT with ZnPc photosensitizers show a powerful antimicrobial activity against the most frequent pathogens in endodontic infections and this method for inactivation of pathogens may be used with sucsses for treatment of the bacterial biofilms in the root canals.

  13. Spectra/Por DispoDialyzer

    E-print Network

    Lebendiker, Mario

    Spectra/Por ® DispoDialyzer® Micro DispoDialyzer® Float-A-Lyzer® READY-TO-USE DIALYSIS DEVICES dialysis. READY TO USE · No assembly of closures, buoys or weights · No membrane preparation · Includes or sulfide contaminants · Color-coded cap for MWCO's LARGEST SELECTION: 148 AVAILABLE 3 Dialysis Device

  14. In Vitro Activity of AZD0914, a Novel Bacterial DNA Gyrase/Topoisomerase IV Inhibitor, against Clinically Relevant Gram-Positive and Fastidious Gram-Negative Pathogens.

    PubMed

    Biedenbach, Douglas J; Huband, Michael D; Hackel, Meredith; de Jonge, Boudewijn L M; Sahm, Daniel F; Bradford, Patricia A

    2015-10-01

    AZD0914, a new spiropyrimidinetrione bacterial DNA gyrase inhibitor with a novel mode of inhibition, has activity against bacterial species commonly cultured from patient infection specimens, including fluoroquinolone-resistant isolates. This study assessed the in vitro activity of AZD0914 against key Gram-positive and fastidious Gram-negative clinical isolates collected globally in 2013. AZD0914 demonstrated potent activity, with MIC90s for AZD0914 of 0.25 mg/liter against Staphylococcus aureus (n = 11,680), coagulase-negative staphylococci (n = 1,923), streptococci (n = 4,380), and Moraxella catarrhalis (n = 145), 0.5 mg/liter against Staphylococcus lugdunensis (n = 120) and Haemophilus influenzae (n = 352), 1 mg/liter against Enterococcus faecalis (n = 1,241), and 2 mg/liter against Haemophilus parainfluenzae (n = 70). The activity against Enterococcus faecium was more limited (MIC90, 8 mg/liter). The spectrum and potency of AZD0914 included fluoroquinolone-resistant isolates in each species group, including methicillin-resistant staphylococci, penicillin-resistant streptococci, vancomycin-resistant enterococci, ?-lactamase-producing Haemophilus spp., and M. catarrhalis. Based on these in vitro findings, AZD0914 warrants further investigation for its utility against a variety of Gram-positive and fastidious Gram-negative bacterial species. PMID:26195518

  15. Vaccination and otitis media.

    PubMed

    Karma, P

    2002-01-01

    Acute otitis media (AOM) caused by Streptococcus pneumoniae, Haemophilus influenzae or Moraxella catarrhalis may induce specific systemic and/or local immune responses, which may protect from otitis media caused by the same bacteria. However, earlier clinical trials with pneumococcal capsular polysaccharide vaccines have not been successful in preventing AOM. Recently developed pneumococcal polysaccharide-protein conjugates proved immunogenic even in infants, and a heptavalent pneumococcal CRM 197 conjugate vaccine gave a 57% reduction in the number of pneumococcal AOM episodes caused by the vaccine serotypes in infants in Finland. H. influenzae causing AOM is noncapsulated, and like M. catarrhalis, calls for another kind of vaccine development. Suitable vaccine candidates are not yet available but are under development and being tested for immunogenicity and safety. In some trials influenza vaccines have shown protection from AOM during respective viral epidemics. Passive immunoprophylaxis might be an important alternative for immunocompromised children, although this approach has not been successful so far. Mucosal immunization and the advent of DNA and gene technology will open new interesting prospects in the future. PMID:12021498

  16. Impaired Innate COPD Alveolar Macrophage Responses and Toll-Like Receptor-9 Polymorphisms

    PubMed Central

    Berenson, Charles S.; Kruzel, Ragina L.; Wrona, Catherine T.; Mammen, Manoj J.; Sethi, Sanjay

    2015-01-01

    Background Dysfunctional innate responses of alveolar macrophages to nontypeable Haemophilus influenzae, Moraxella catarrhalis and Streptococcus pneumoniae contribute to morbidity in chronic obstructive pulmonary disease (COPD). Our earlier studies discovered impaired COPD alveolar macrophage responses to Toll-like receptor (TLR) ligands of nontypeable H. influenzae and provide rationale for further evaluation of TLR signaling. While the role of TLR single nucleotide polymorphisms is increasingly recognized in inflammatory diseases, TLR single nucleotide polymorphisms in COPD have only recently been explored. We hypothesized that specific TLR polymorphisms are associated with dysfunctional innate immune COPD alveolar macrophage responses and investigated polymorphisms of TLR2(Arg753Gln), TLR4(Thr399Ile; Asp299Gly), and TLR9(T1486C; T1237C). Methods DNA was purified from cells of 1) healthy nonsmokers (n = 20); 2) COPD ex-smokers (n = 83); 3) COPD active smokers (n = 93). DNA amplifications (polymerase chain reaction) were performed for each SNP. Alveolar macrophages from each group were incubated with nontypeable H. influenzae, M. catarrhalis and S. pneumoniae. Cytokine induction of macrophage supernatants was measured and the association with TLR single nucleotide polymorphism expression was determined. Results No significant inter-group differences in frequency of any TLR SNP existed. However both TLR9 single nucleotide polymorphisms were expressed in high frequency. Among COPD ex-smokers, diminished IL-8 responsiveness to nontypeable H. influenzae, M. catarrhalis and S. pneumoniae was strongly associated with carriage of TLR9(T1237C) (p = 0.02; p = 0.008; p = 0.02), but not TLR9(T1486C). Carriage of TLR9(T1237C), but not TLR9(T1486C), correlated with diminished FEV1%predicted (p = 0.037). Conclusion Our results demonstrate a notable association of TLR9(T1237C) expression with dysfunctional innate alveolar macrophage responses to respiratory pathogens and with severity of COPD. PMID:26361369

  17. The Viriato study: update on antimicrobial resistance of microbial pathogens responsible for community-acquired respiratory tract infections in Portugal.

    PubMed

    Melo-Cristino, José; Santos, Letícia; Silva-Costa, Catarina; Friães, Ana; Pinho, Marcos D; Ramirez, Mário

    2010-06-29

    The Viriato study is a prospective, multicentre laboratory-based surveillance study of antimicrobial susceptibility in which 30 microbiology laboratories throughout Portugal are asked to isolate, identify and submit to a central laboratory for testing Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis responsible for community-acquired lower respiratory tract infections and Streptococcus pyogenes from tonsillitis. To monitor changes in antimicrobial resistance patterns of these frequent respiratory pathogens. Susceptibility was determined by disk diffusion (Kirby-Bauer) or using Etest strips following the Clinical and Laboratory Standards Institute guidelines. From 1999 to 2007 over 13 900 isolates were analysed. Among S. pneumoniae penicillin non-susceptibility decreased from 25% in 1999 to 18% in 2007 (p = 0.002) but resistance to macrolides showed a steady increase, reaching 20% in the last 6 years. Resistance to amoxicillin and the quinolones remained stable and very low (1-2%) throughout the study period. Antimicrobial resistance among H. influenzae and M. catarrhalis remained stable. The most significant resistance was to ampicillin, of 10-12% and greater than 80%, respectively, as a result of the production of beta-lactamases. Macrolide resistance among S. pyogenes was stable during 1999-2003 (20-23%) but after 2003 there was a steady decline in resistance, which in 2007 reached 10%. The Viriato surveillance study showed that penicillin remains the most active antimicrobial agent against S. pyogenes causing tonsillitis, and amoxicillin-clavulanate and the quinolones are the most active in vitro simultaneously against S. pneumoniae, H. influenzae and M. catarrhalis responsible for community-acquired lower respiratory tract infections in Portugal. PMID:20590169

  18. Effects of Vaccination with 10-Valent Pneumococcal Non-Typeable Haemophilus influenza Protein D Conjugate Vaccine (PHiD-CV) on the Nasopharyngeal Microbiome of Kenyan Toddlers

    PubMed Central

    Feazel, Leah M.; Santorico, Stephanie A.; Robertson, Charles E.; Bashraheil, Mahfudh; Scott, J. Anthony G.

    2015-01-01

    Objective Pneumococcal conjugate vaccines reduce the prevalence of vaccine serotypes carried in the nasopharynx. Because this could alter carriage of other potential pathogens, we assessed the nasopharyngeal microbiome of children who had been vaccinated with 10-valent pneumococcal non-typeable Haemophilus influenzae protein-D conjugate vaccine (PHiD-CV). Methods Profiles of the nasopharyngeal microbiota of 60 children aged 12-59 months, who had been randomized to receive 2 doses of PHiD-CV (n=30) or Hepatitis A vaccine (n=30) 60 days apart, were constructed by 16S rRNA gene pyrosequencing of swab specimens collected before vaccination and 180 days after dose 1. Results Prior to vaccination, Moraxella catarrhalis (median of 12.3% of sequences/subject), Streptococcus pneumoniae (4.4%) and Corynebacterium spp. (5.6%) were the most abundant nasopharyngeal bacterial species. Vaccination with PHiD-CV did not significantly alter the species composition, abundance, or prevalence of known pathogens. Distinct microbiomes were identified based on the abundances of Streptococcus, Moraxella, and Haemophilus species. These microbiomes shifted in composition over the study period and were independent of age, sex, school attendance, antibiotic exposure, and vaccination. Conclusions Vaccination of children with two doses of PHiD-CV did not significantly alter the nasopharyngeal microbiome. This suggests limited replacement carriage with pathogens other than non-vaccine strains of S. pneumoniae. Trial Registration clinicaltrials.gov NCT01028326 PMID:26083474

  19. Long-term impact of pneumococcal polysaccharide vaccination on nasopharyngeal carriage in children previously vaccinated with various pneumococcal conjugate vaccine regimes.

    PubMed

    Boelsen, Laura K; Dunne, Eileen M; Lamb, Karen E; Bright, Kathryn; Cheung, Yin Bun; Tikoduadua, Lisi; Russell, Fiona M; Mulholland, E Kim; Licciardi, Paul V; Satzke, Catherine

    2015-10-13

    Previously, the Fiji Pneumococcal Project (FiPP) evaluated reduced dose immunization schedules that incorporated pneumococcal protein conjugate and/or polysaccharide vaccine (PCV7 and 23vPPV, respectively). Immune hyporesponsiveness was observed in children vaccinated with 23vPPV at 12 months of age compared with children who did not receive 23vPPV. Here we assess the long-term impact of 23vPPV vaccination on nasopharyngeal carriage rates and densities of Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus and Moraxella catarrhalis. Nasopharyngeal swabs (n=194) were obtained from healthy children who participated in FiPP (now aged 5-7 years). S. pneumoniae were isolated and identified by standard culture-based methods, and serotyped using latex agglutination and the Quellung reaction. Carriage rates and densities of S. pneumoniae, H. influenzae, S. aureus and M. catarrhalis were determined using real-time quantitative PCR. There were no differences in the rate or density of S. pneumoniae, H. influenzae or M. catarrhalis carriage by PCV7 dose or 23vPPV vaccination in the vaccinated participants overall. However, differences were observed between the two main ethnic groups: Fijian children of Indian descent (Indo-Fijian) were less likely to carry S. pneumoniae, H. influenzae and M. catarrhalis, and there was evidence of a higher carriage rate of S. aureus compared with indigenous Fijian (iTaukei) children. Polysaccharide vaccination appeared to have effects that varied between ethnic groups, with 23vPPV vaccination associated with a higher carriage rate of S. aureus in iTaukei children, while there was a lower carriage rate of S. pneumoniae associated with 23vPPV vaccination in Indo-Fijian children. Overall, polysaccharide vaccination had no long-term impact on pneumococcal carriage, but may have impacted on S. aureus carriage and have varying effects in ethnic groups, suggesting current WHO vaccine schedule recommendations against the use of 23vPPV in children under two years of age are appropriate. PMID:26232540

  20. Detección y estudio mediante Fluorescencia Inducida por Láser de radicales libres formados por Disociación Multifotónica Infrarroja

    NASA Astrophysics Data System (ADS)

    Santos, M.; Díaz, L.; Torresano, J. A.; Rubio, L.; Samoudi, B.

    Una de las principales aplicaciones actuales de los procesos de disociación multifotónica inducidos por radiación láser infrarroja (DMI) es la producción de radiales libres, con el fin de estudiar sus propiedades cinéticas y espectroscópicas. La disociación de moléculas poliatómicas en el IR con láseres de CO2 tiene lugar desde la superficie de energía molecular mas baja y conduce generalmente a la formación de fragmentos en el estado electrónico fundamental, con diversos grados de excitación vibracional. En el Grupo de Procesos Multifotónicos del Instituto de Estructura de la Materia del C.S.I.C. hemos puesto a punto la técnica de Fluorescencia Inducida por Láser (LIF) para la detección y análisis en tiempo real de los fragmentos producidos en la DMI inducida mediante uno o dos campos láseres de diferentes longitudes de onda. Objetivos de nuestro trabajo han sido el estudio de los canales de disociación mayoritarios y de las especies transitoria producidas, así como de la distribución de energía interna con que éstas son generadas. En particular hemos detectado mediante LIF las especies: C2, CF, CH, SiH2, CF2, CH2, SiHCl, y CF3 a partir de la disociación de, entre otras, las siguientes moléculas: C2H3Br, C3F6, C4H8Si, C2H5ClSi y CH5ClSi. En este trabajo presentamos algunos de los resultados obtenidos mediante el estudio por LIF de estos radicales: estudio temporal de la señal LIF obtenida con determinación de tiempos de vida, espectros de excitación y fluorescencia, temperaturas vibracionales de formación, variación de la intensidad LIF con el tiempo de retraso entre los láseres de disociación y prueba, etc.

  1. POR QUE LA INFERENCIA ESTADISTICA M. A. Gomez Villegas

    E-print Network

    Gómez Villegas, Miguel Ángel

    POR QU´E LA INFERENCIA ESTADISTICA BAYESIANA M. A. G´omez Villegas 1 Introducci´on En este art´iculo se pretende justificar por qu´e se debe utilizar la aproximaci´on bayesiana a la inferencia estad´istica. Como anunciaba Lindley en el primer Congreso Internacional de Estad´istica Bayesiana, falta menos para

  2. Infección por VIH y el riesgo de cáncer

    Cancer.gov

    Hoja informativa que describe el mayor riesgo de ciertos tipos de cánceres que presentan las personas con infección por VIH. Dichos cánceres son el sarcoma de Kaposi, los linfomas de Hodgkin y no Hodgkin, y los cánceres de ano, de cérvix, de hígado y de pulmón. Trata también de lo que las personas con infección por VIH pueden hacer para reducir su riesgo de cáncer o para detectar temprano la enfermedad.

  3. Association of targeted multiplex PCR with resequencing microarray for the detection of multiple respiratory pathogens

    PubMed Central

    Shen, Hongwei; Zhu, Bingqing; Wang, Shulian; Mo, Haolian; Wang, Ji; Li, Jin; Zhang, Chen; Zeng, Huashu; Guan, Li; Shi, Weixian; Zhang, Yong; Ma, Xuejun

    2015-01-01

    A large number of viral and bacterial organisms are responsible for community-acquired pneumonia (CAP) which contributes to substantial burden on health management. A new resequencing microarray (RPM-IVDC1) associated with targeted multiplex PCR was recently developed and validated for multiple respiratory viruses detection and discrimination. In this study, we evaluated the capability of RPM-IVDC1 for simultaneous identification of multiple viral and bacterial organisms. The nasopharyngeal aspirates (NPAs) of 110 consecutive CAP patients, aged from 1 month to 96 years old, were collected from five distinct general hospitals in Beijing during 1-year period. The samples were subjected to the RPM-IVDC1 established protocol as compared to a real-time PCR (qRT-PCR), which was used as standard. The results of virus detection were consistent with those previously described. A total of 37 of Streptococcus pneumoniae, 14 of Haemophilus influenzae, 10 of Mycoplasma pneumoniae, two of Klebsiella pneumoniae and one of Moraxella catarrhalis were detected by RPM-IVDC1. The sensitivities and specificities were compared with those of qRT-PCR for S. pneumoniae (100, 100%, respectively), H. influenzae (92.3, 97.9%, respectively), M. pneumoniae (69.2, 99.0%, respectively), K. pneumoniae (100, 100%, respectively), and M. catarrhalis (100, 100%, respectively). Additional 22 of Streptococcus spp., 24 of Haemophilus spp. and 16 of Neisseria spp. were identified. In addition, methicillin-resistant and carbapenemases allele were also found in nine of Staphylococcus spp. and one of K. pneumoniae, respectively. These results demonstrated the capability of RPM-IVDC1 for simultaneous detection of broad-spectrum respiratory pathogens in complex backgrounds and the advantage of accessing to the actual sequences, showing great potential use of epidemic outbreak investigation. The detection results should be carefully interpreted when introducing this technique in the clinical diagnostics. PMID:26074910

  4. Carriage of Streptococcus pneumoniae and Other Respiratory Bacterial Pathogens in Low and Lower-Middle Income Countries: A Systematic Review and Meta-Analysis

    PubMed Central

    Adegbola, Richard A.; DeAntonio, Rodrigo; Hill, Philip C.; Roca, Anna; Usuf, Effua; Hoet, Bernard; Greenwood, Brian M.

    2014-01-01

    Background Infection with Streptococcus pneumoniae is a major cause of childhood morbidity and mortality worldwide, especially in low income countries where pneumococcal conjugate vaccines (PCVs) are still underused. In countries where PCVs have been introduced, much of their efficacy has resulted from their impact on nasopharyngeal carriage in vaccinated children. Understanding the epidemiology of carriage for S. pneumoniae and other common respiratory bacteria in developing countries is crucial for implementing appropriate vaccination strategies and evaluating their impact. Methods and Findings We have systematically reviewed published studies reporting nasopharyngeal or oropharyngeal carriage of S. pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus, and Neisseria meningitidis in children and adults in low and lower-middle income countries. Studies reporting pneumococcal carriage for healthy children <5 years of age were selected for a meta-analysis. The prevalences of carriage for S. pneumoniae, H. influenzae, and M. catarrhalis were generally higher in low income than in lower-middle income countries and were higher in young children than in adults. The prevalence of S. aureus was high in neonates. Meta-analysis of data from young children before the introduction of PCVs showed a pooled prevalence estimate of 64.8% (95% confidence interval, 49.8%–76.1%) in low income countries and 47.8% (95% confidence interval, 44.7%–50.8%) in lower-middle income countries. The most frequent serotypes were 6A, 6B, 19A, 19F, and 23F. Conclusions In low and lower-middle income countries, pneumococcal carriage is frequent, especially in children, and the spectrum of serotypes is wide. However, because data are limited, additional studies are needed to adequately assess the impact of PCV introduction on carriage of respiratory bacteria in these countries. PMID:25084351

  5. Common Cell Shape Evolution of Two Nasopharyngeal Pathogens

    PubMed Central

    Veyrier, Frédéric J.; Biais, Nicolas; Morales, Pablo; Belkacem, Nouria; Guilhen, Cyril; Ranjeva, Sylvia; Sismeiro, Odile; Péhau-Arnaudet, Gérard; Rocha, Eduardo P.; Werts, Catherine

    2015-01-01

    Respiratory infectious diseases are the third cause of worldwide death. The nasopharynx is the portal of entry and the ecological niche of many microorganisms, of which some are pathogenic to humans, such as Neisseria meningitidis and Moraxella catarrhalis. These microbes possess several surface structures that interact with the actors of the innate immune system. In our attempt to understand the past evolution of these bacteria and their adaption to the nasopharynx, we first studied differences in cell wall structure, one of the strongest immune-modulators. We were able to show that a modification of peptidoglycan (PG) composition (increased proportion of pentapeptides) and a cell shape change from rod to cocci had been selected for along the past evolution of N. meningitidis. Using genomic comparison across species, we correlated the emergence of the new cell shape (cocci) with the deletion, from the genome of N. meningitidis ancestor, of only one gene: yacF. Moreover, the reconstruction of this genetic deletion in a bacterium harboring the ancestral version of the locus together with the analysis of the PG structure, suggest that this gene is coordinating the transition from cell elongation to cell division. Accompanying the loss of yacF, the elongation machinery was also lost by several of the descendants leading to the change in the PG structure observed in N. meningitidis. Finally, the same evolution was observed for the ancestor of M. catarrhalis. This suggests a strong selection of these genetic events during the colonization of the nasopharynx. This selection may have been forced by the requirement of evolving permissive interaction with the immune system, the need to reduce the cellular surface exposed to immune attacks without reducing the intracellular storage capacity, or the necessity to better compete for adhesion to target cells. PMID:26162030

  6. Impact of poor compliance with levofloxacin and moxifloxacin on respiratory tract infection antimicrobial efficacy: a pharmacokinetic/pharmacodynamic simulation study.

    PubMed

    Carral, N; Lukas, J C; Oteo, I; Suarez, E

    2015-01-01

    The purpose of this report was to assess the impact of poor compliance on the efficacy of levofloxacin (LFX) and moxifloxacin (MOX), two fluoroquinolones with different pharmacokinetic (PK) and pharmacodynamic (PD) properties, in respiratory infections. The fAUC0-24h and fAUC0-24h/MIC90 ratio, a PK/PD index predictive of bacterial eradication, were extracted from previously described population PK models for LFX and MOX. The MIC90 was according to EUCAST. Monte Carlo simulations were used with LFX 500 mg every 24h (q24 h) or every 12h (q12h), LFX 750 mg q24 h and MOX 400mg q24 h in non-compliance scenarios to derive the proportion of patients achieving target ratios of fAUC0-24h/MIC90>33.8 for Streptococcus pneumoniae and >100 for Haemophilus influenzae and Moraxella catarrhalis (PTA>90%). In non-adherent dosing scenarios, LFX 500 mg q24 h was not able to reach the PK/PD index guaranteeing clinical efficacy. With LFX 500 mg q12 h or 750 mg q24 h, this probability was maintained although patients can take the dose with delays of up to 12h and 11h, respectively, for the three bacterial types. With MOX 400mg q24 h, the probability of achieving this PK/PD index is maintained with delay in dosing up to 16h. In conclusion, LFX 500 mg q24 h is the least robust treatment against S. pneumoniae, H. influenzae and M. catarrhalis in a non-adherence situation. A good choice is LFX 500 mg q12h, but in order to favour patient adherence, LFX 750 mg q24 h or MOX 400mg q24h appears as more appropriate. PMID:25450804

  7. System design description for portable 1,000 CFM exhauster Skids POR-007/Skid E and POR-008/Skid F

    SciTech Connect

    Nelson, O.D.

    1998-07-25

    The primary purpose of the two 1,000 CFM Exhauster Skids, POR-007-SKID E and POR-008-SKID F, is to provide backup to the waste tank primary ventilation systems for tanks 241-C-106 and 241-AY-102, and the AY-102 annulus in the event of a failure during the sluicing of tank 241-C-106 and subsequent transfer of sluiced waste to 241-AY-102. This redundancy is required since both of the tank ventilation systems have been declared as Safety Class systems.

  8. Informe a la Nación de mortalidad por cáncer sigue bajando

    Cancer.gov

    El Informe Anual a la Nación sobre el Estado del Cáncer, de 1975 a 2009, indica que los índices generales de mortalidad por cáncer siguen bajando en los Estados Unidos en hombres y mujeres, entre todos los grupos raciales y étnicos principales y para todo

  9. Se evitaron casi 800 000 muertes por descenso del tabaquismo

    Cancer.gov

    Programas y estrategias de control del tabaco del siglo XX fueron responsables de la prevención de más de 795 000 muertes por cáncer de pulmón en Estados Unidos de 1975 al 2000. Si todo el tabaquismo en este país hubiera cesado después de la publicación d

  10. Pato Carretero El Pato Carretero se distribuye por

    E-print Network

    Cuervo, Andrés

    479 Familia Anatidae Pato Carretero El Pato Carretero se distribuye por el oriente de Suramérica Accipitridae Azor Collarejo Esta especie se distribuye desde el suroccidente de Venezuela hasta Perú, sobre ambos flancos de los Andes (Ellis y Álvarez 1994). En Colombia se encuentra en la vertiente Pacífica, en

  11. Identification of Porphyromonas gingivalis proteins secreted by the Por secretion system.

    PubMed

    Sato, Keiko; Yukitake, Hideharu; Narita, Yuka; Shoji, Mikio; Naito, Mariko; Nakayama, Koji

    2013-01-01

    The Gram-negative bacterium Porphyromonas gingivalis possesses a number of potential virulence factors for periodontopathogenicity. In particular, cysteine proteinases named gingipains are of interest given their abilities to degrade host proteins and process other virulence factors such as fimbriae. Gingipains are translocated on the cell surface or into the extracellular milieu by the Por secretion system (PorSS), which consists of a number of membrane or periplasmic proteins including PorK, PorL, PorM, PorN, PorO, PorP, PorQ, PorT, PorU, PorV (PG27, LptO), PorW and Sov. To identify proteins other than gingipains secreted by the PorSS, we compared the proteomes of P. gingivalis strains kgp rgpA rgpB (PorSS-proficient strain) and kgp rgpA rgpB porK (PorSS-deficient strain) using two-dimensional gel electrophoresis and peptide-mass fingerprinting. Sixteen spots representing 10 different proteins were present in the particle-free culture supernatant of the PorSS-proficient strain but were absent or faint in that of the PorSS-deficient strain. These identified proteins possessed the C-terminal domains (CTDs), which had been suggested to form the CTD protein family. These results indicate that the PorSS is used for secretion of a number of proteins other than gingipains and that the CTDs of the proteins are associated with the PorSS-dependent secretion. PMID:23075153

  12. DISTRIBUCION DE PERSONAL INVESTIGADOR DE PLANTILLA por reas cientficas, escalas y sexo.

    E-print Network

    Fitze, Patrick

    DISTRIBUCION DE PERSONAL INVESTIGADOR DE PLANTILLA DEL CSIC por áreas científicas, escalas y sexo (totales por escla y sexo) Todas areas HOMBRES MUJERES TOTAL % MUJERES PI 398 71 469 15% IC 406 182 588 31 Distribución de personal (total 2343) por escala y sexo 17% 17% 34% 21% 8% 3% PI IC CT CT IC PI Hombres por

  13. Silla de ruedas inteligente controlada por Voz Alcubierre, Minguez, Montesano Montano, Saz, Lleida

    E-print Network

    Minguez, Javier

    Silla de ruedas inteligente controlada por Voz Alcubierre, Minguez, Montesano Montano, Saz, Lleida Primer Congreso Internacional de Domótica, Robótica y Silla de Ruedas Inteligente Controlada por Voz J silla de ruedas con capacidad de guiado autónomo controlada por voz. La silla robotizada está equipada

  14. 1/4 XXII CBEB 2010 MEDIO DA VARIABILIDADE DO VOLUME SISTLICO POR MEIO DE

    E-print Network

    Carvalho, João Luiz

    1/4 XXII CBEB 2010 MEDIÇÃO DA VARIABILIDADE DO VOLUME SISTÓLICO POR MEIO DE RESSONÂNCIA MAGNÉTICA do débito cardíaco por meio de ressonância magnética (RM) de fluxo [2,3]. Embora resultados técnica para medição não invasiva do volume sistólico batimento a batimento por meio de RM de fluxo

  15. 1/4 CBEB 2008 MEDIO DE TAXA DE CISALHAMENTO INTRAVASCULAR POR MEIO

    E-print Network

    Carvalho, João Luiz

    1/4 CBEB 2008 MEDIÇÃO DE TAXA DE CISALHAMENTO INTRAVASCULAR POR MEIO DE RESSONÂNCIA MAGNÉTICA João perfil de velocidade [6], medido próximo à parede por meio de técnicas como ultrassonografia-Doppler [7 na bifurcação carotídea por meio de ressonância magnética, usando aquisições em espiral com

  16. Otopathogens Detected in Middle Ear Fluid Obtained during Tympanostomy Tube Insertion: Contrasting Purulent and Non-Purulent Effusions

    PubMed Central

    Holder, Robert C.; Kirse, Daniel J.; Evans, Adele K.; Whigham, Amy S.; Peters, Timothy R.; Poehling, Katherine A.; Swords, William E.; Reid, Sean D.

    2015-01-01

    Otitis media is a prominent disease among children. Previous literature indicates that otitis media is a polymicrobial disease, with Haemophilus influenzae, Streptococcus pneumoniae, Alloiococcus otitidis and Moraxella catarrhalis being the most commonly associated bacterial pathogens. Recent literature suggests that introduction of pneumococcal conjugate vaccines has had an effect on the etiology of otitis media. Using a multiplex PCR procedure, we sought to investigate the presence of the aforementioned bacterial pathogens in middle ear fluid collected from children undergoing routine tympanostomy tube placement at Wake Forest Baptist Medical Center during the period between January 2011 and March 2014. In purulent effusions, one or more bacterial organisms were detected in ~90% of samples. Most often the presence of H. influenzae alone was detected in purulent effusions (32%; 10 of 31). In non-purulent effusions, the most prevalent organism detected was A. otitidis (26%; 63 of 245). Half of the non-purulent effusions had none of these otopathogens detected. In purulent and non-purulent effusions, the overall presence of S. pneumoniae was lower (19%; 6 of 31, and 4%; 9 of 245, respectively) than that of the other pathogens being identified. The ratio of the percentage of each otopathogen identified in purulent vs. non-purulent effusions was >1 for the classic otopathogens but not for A. otitidis. PMID:26039250

  17. Antimicrobial activity of preparation Bioaron C.

    PubMed

    Gawron-Gzella, Anne; Michalak, Anna; K?dzia, Anna

    2014-01-01

    The antimicrobial activity of sirupus Bioaron C, a preparation, whose main ingredient is an extract from the leaves of Aloe arborescens, was tested against different microorganisms isolated from patients with upper respiratory tract infections. The experiments were performed on 40 strains: 20 strains of anaerobic bacteria, 13 strains of aerobic bacteria and 7 strains of yeast-like fungi from the genus Candida and on 18 reference strains (ATCC). The antimicrobial activity of Bioaron C (MBC and MFC) was determined at undiluted concentration. Bioaron C proved to be very effective against the microorganisms causing infections. At the concentration recommended by the producer, the preparation showed biocidal activity (MBC, MFC) against the strains of the pathogenic microorganisms, which cause respiratory infections most frequently, including, among others, Peptostreptococcus anaerobius, Parvimonas micra, Staphylococcus aureus, Streptococcus pyogenes, Streptococcus pneumoniae, Streptococcus anginosus, Haemophilus influenzae, Moraxella catarrhalis, Pseudomonas aeruginosa and Candida albicans, already after 15 min. The MIC of Bioaron C against most of the tested microorganisms was 5 to 100 times lower than the usually applied concentration. The great antimicrobial activity means that the preparation may be used in the prevention and treatment of infections of the upper respiratory tract. Bioaron C may be an alternative or complement to classical therapy, especially in children. PMID:25362808

  18. Inhibitory and Bactericidal Effects of Hydrogen Peroxide Production by Streptococcus pneumoniae on Other Inhabitants of the Upper Respiratory Tract

    PubMed Central

    Pericone, Christopher D.; Overweg, Karin; Hermans, Peter W. M.; Weiser, Jeffrey N.

    2000-01-01

    An inverse correlation between colonization of the human nasopharynx by Streptococcus pneumoniae and Haemophilus influenzae, both common upper respiratory pathogens, has been reported. Studies were undertaken to determine if either of these organisms produces substances which inhibit growth of the other. Culture supernatants from S. pneumoniae inhibited growth of H. influenzae, whereas culture supernatants from H. influenzae had no effect on the growth of S. pneumoniae. Moreover, coculture of S. pneumoniae and H. influenzae led to a rapid decrease in viable counts of H. influenzae. The addition of purified catalase prevented killing of H. influenzae in coculture experiments, suggesting that hydrogen peroxide may be responsible for this bactericidal activity. H. influenzae was killed by concentrations of hydrogen peroxide similar to that produced by S. pneumoniae. Hydrogen peroxide is produced by the pneumococcus through the action of pyruvate oxidase (SpxB) under conditions of aerobic growth. Both an spxB mutant and a naturally occurring variant of S. pneumoniae, which is downregulated in SpxB expression, were unable to kill H. influenzae. A catalase-reversible inhibitory effect of S. pneumoniae on the growth of the respiratory tract pathogens Moraxella catarrhalis and Neisseria meningitidis was also observed. Elevated hydrogen peroxide production, therefore, may be a means by which S. pneumoniae is able to inhibit a variety of competing organisms in the aerobic environment of the upper respiratory tract. PMID:10858213

  19. Antibacterial, antioxidant and hypoglycaemic effects of Thymus capitatus (L.) Hoffmanns. et Link leaves' fractions.

    PubMed

    Iauk, Liliana; Acquaviva, Rosaria; Mastrojeni, Silvana; Amodeo, Andrea; Pugliese, Michela; Ragusa, Monica; Loizzo, Monica R; Menichini, Francesco; Tundis, Rosa

    2015-06-01

    The aim of this study was to examine the bioactivity of the methanol fraction (MF) and n-hexane fraction (HF) of Thymus capitatus leaves in relation to their constituents analysed by gas chromatography and gas chromatography-mass spectrometry. The effects of T. capitatus on the growth of pathogenic bacteria associated with respiratory diseases (13 gram-positive and 4 gram-negative) were determined using a microdilution method. The MF was particularly effective on Streptococcus pneumoniae and Moraxella catarrhalis. The antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), ferric-reducing antioxidant power and ?-carotene bleaching assays. A strong activity using ?-carotene bleaching test was observed with the MF (IC50 of 0.7??g/mL after 30?min of incubation). In the hypoglycaemic test, a selective ?-amylase inhibitory activity was detected with the HF begging the most active (IC50 of 422.5??g/mL). T. capitatus may represent a source of natural bioactive compounds. PMID:25032744

  20. [Incidence of selected bacterial pathogens of the respiratory tract in patients with bronchial asthma].

    PubMed

    Ekiel, Alicja; Friedek, Daniela; Szulakowski, Patryk; Romanik, Ma?gorzata; Rogala-Zawada, Danuta; Wilk, Iwona

    2002-01-01

    Chronic bacterial infections intensify the reactivity of bronchi and aggravate the course and the control of asthma. They cause the disorders of both function and the structure of respiratory epithelium. Not only structural elements of bacteria but also their toxins intensify the release of mediators of the inflammatory reaction (leucotriens, histamine, IL1, IL4, IL6, IL8, TNF alpha). The aim of our research is to determine the prevalence of microorganisms, which can have an influence on the course of asthma. Moraxella catarrhalis has been the most frequent isolated pathogen (23.7%) in patients with bronchial asthma. We have received only individual isolations of Streptococcus pneumoniae and Haemophilus influenzae strains. Bacterial flora of the upper respiratory tract in patients with bronchial asthma has been more diverse in comparison with microflora of airways in healthy subjects. The significant percentage of Candida isolation in asthmatics (over 30% in bronchial tree secretion) poses the high risk of incidence of mycotic complications of inhaled steroids. In patients with asthma bronchial tree secretion is more valuable diagnostic material than pharyngeal swab. PMID:12043306

  1. [Management of COPD exacerbations: from primary care to hospitalization].

    PubMed

    Jouneau, Stéphane; Brinchault, Graziella; Desrues, Benoît

    2014-12-01

    The Société de pneumologie de langue française defines acute exacerbation of chronic obstructive pulmonary disease (AE COPD) as an increase in daily respiratory symptoms, basically duration ? 48h or need for treatment adjustment. Etiology of EA COPD are mainly infectious, viral (rhinovirus, influenzae or parainfluenzae virus, coronavirus, adenovirus and respiratory syncytial virus) or bacterial (Haemophilus influenzae, Streptococcus pneumoniae, or Moraxella catarrhalis). Pollutant exposure can also lead to AE COPD, such as NO2, SO2, ozone or particulates (PM10 and PM2.5). In 30% the etiology remains unknown. Differential diagnoses of AE COPD include infectious pneumonia, pneumothorax, acute heart failure and pulmonary embolism. Presences of signs of severity impose hospitalization: signs of respiratory distress, shock, acute confusion but also fragile patients, insufficient home support or absence of response to initial treatment. AE COPD treatments consist on increase in bronchodilators, chest physiotherapy, and antibiotics if sputum is frankly purulent. Systemic corticosteroids should not be systematic. Recommended dose is 0.5 mg/kg on short course (5-7 days). During hospitalization, oxygen supplementation and thromboprophylaxis could be prescribed. The main interest in non-invasive ventilation is persistent hypercapnia despite optimal medical management. During ambulatory management or hospitalization, clinical assessment at 48-72 h is mandatory. PMID:25451635

  2. Synthesis and structure-activity relationships of novel 9-oxime acylides with improved bactericidal activity.

    PubMed

    Han, Xu; Lv, Wei; Guo, Si-Yang; Cushman, Mark; Liang, Jian-Hua

    2015-10-01

    9-Oxime acylides have different SAR and binding modes from 9-oxime ketolides. An aminopyridyl or carbamoylpyridyl group anchored at the end of the 9-oxime 2-propargyl group is beneficial for antimicrobial activity. Both the 2-pyridyl and 3-pyridyl groups derived from 3-OH have stacking interactions with the base pair G2505/C2610 (Escherichia coli numbering) of the bacterial rRNA. Compounds 3 presented characteristic features that belong to bactericidal agents when used against constitutive-erm resistant Staphylococcus aureus, susceptible and mef-encoded Streptococcus pneumoniae, inducible-erm resistant Streptococcus pyogenes, and Moraxella catarrhalis. A docking model indicated that the carbamoylpyridyl group of 3h may hydrogen bond to G2061 in addition to ?-? stacking over the adenine of A2062 that proved to gate the tunnel for the egress of the nascent peptide. This study suggests that the 9-oxime acylides possess a bactericidal mechanism that is different from the traditional near-complete inhibition of protein synthesis. These studies provide a foundation for the rational design of macrolide antibiotics. PMID:26349628

  3. Targeting cystalysin, a virulence factor of treponema denticola-supported periodontitis.

    PubMed

    Spyrakis, Francesca; Cellini, Barbara; Bruno, Stefano; Benedetti, Paolo; Carosati, Emanuele; Cruciani, Gabriele; Micheli, Fabrizio; Felici, Antonio; Cozzini, Pietro; Kellogg, Glen E; Voltattorni, Carla Borri; Mozzarelli, Andrea

    2014-07-01

    Cystalysin from Treponema denticola is a pyridoxal 5'-phosphate dependent lyase that catalyzes the formation of pyruvate, ammonia, and sulfide from cysteine. It is a virulence factor in adult periodontitis because its reaction contributes to hemolysis, which sustains the pathogen. Therefore, it was proposed as a potential antimicrobial target. To identify specific inhibitors by structure-based in silico methods, we first validated the crystal structure of cystalysin as a reliable starting point for the design of ligands. By using single-crystal absorption microspectrophotometry, we found that the enzyme in the crystalline state, with respect to that in solution, exhibits: 1)?the same absorption spectra for the catalytic intermediates, 2)?a close pKa value for the residue controlling the keto enamine ionization, and 3)?similar reactivity with glycine, L-serine, L-methionine, and the nonspecific irreversible inhibitor aminoethoxyvinylglycine. Next, we screened in silico a library of 9357 compounds with the Fingerprints for Ligands and Proteins (FLAP) software, by using the three-dimensional structure of cystalysin as a template. From the library, 17 compounds were selected and experimentally evaluated by enzyme assays and spectroscopic methods. Two compounds were found to competitively inhibit recombinant T.?denticola cystalysin, with inhibition constant (Ki ) values of 25 and 37??M. One of them exhibited a minimum inhibitory concentration (MIC) value of 64??g?mL(-1) on Moraxella catarrhalis ATCC?23246, which proves its ability to cross bacterial membranes. PMID:24616267

  4. Prenatal Diagnosis of Antley-Bixler Syndrome and POR Deficiency

    PubMed Central

    Oldani, Elena; Garel, Catherine; Bucourt, Martine; Carbillon, Lionel

    2015-01-01

    Patient: Female, fetus Final Diagnosis: Antley-Bixler syndrome Symptoms: Craniosynostosis • midface hypoplasia • femoral bowing • radiohumeral synostosis Medication: None Clinical Procedure: Prenatal diagnosis of severe fetal bone disease using detailed ultrasonography and computed tomography Specialty: Obstetrics and Gynecology • Maternal-Fetal Medicine Objective: Rare disease Background: Prenatal diagnosis of severe bone diseases is challenging and requires complete and precise analysis of fetal anomalies to guide genetic investigation and parental counselling. Case Report: We report a rare case of Antley-Bixler syndrome prenatally diagnosed at 26 weeks’ gestation by ultrasound and computed tomography in a 28-year-old woman with a history of early termination of pregnancy for “malposition of the inferior limbs”. The prenatal ultrasound scan showed severe femoral bowing and frontal bossing. Taking into account the high probability of a recurrent severe skeletal disorder, a computed tomography (CT) scan was proposed. CT findings revealed bilateral femora deformation, craniosynostosis, severe midface hypoplasia, and radiohumeral synostosis. These anomalies strongly suggested Antley-Bixler syndrome. Sequencing of the POR gene in the fetus and the parents revealed compound heterozygous mutations in exon 9 and intron 7, both inherited from each parent, and this finding allowed genetic counseling. Conclusions: The first step in the proper prenatal diagnosis of fetal bone disorders is the precise analysis of ultrasonographic images. However, when a severe fetal inherited disorder is strongly suspected in late mid-trimester, CT may be discussed and usefully contribute to diagnosis and prognosis assessment. PMID:26670660

  5. Prenatal Diagnosis of Antley-Bixler Syndrome and POR Deficiency.

    PubMed

    Oldani, Elena; Garel, Catherine; Bucourt, Martine; Carbillon, Lionel

    2015-01-01

    BACKGROUND Prenatal diagnosis of severe bone diseases is challenging and requires complete and precise analysis of fetal anomalies to guide genetic investigation and parental counselling. CASE REPORT We report a rare case of Antley-Bixler syndrome prenatally diagnosed at 26 weeks' gestation by ultrasound and computed tomography in a 28-year-old woman with a history of early termination of pregnancy for "malposition of the inferior limbs". The prenatal ultrasound scan showed severe femoral bowing and frontal bossing. Taking into account the high probability of a recurrent severe skeletal disorder, a computed tomography (CT) scan was proposed. CT findings revealed bilateral femora deformation, craniosynostosis, severe midface hypoplasia, and radiohumeral synostosis. These anomalies strongly suggested Antley-Bixler syndrome. Sequencing of the POR gene in the fetus and the parents revealed compound heterozygous mutations in exon 9 and intron 7, both inherited from each parent, and this finding allowed genetic counseling. CONCLUSIONS The first step in the proper prenatal diagnosis of fetal bone disorders is the precise analysis of ultrasonographic images. However, when a severe fetal inherited disorder is strongly suspected in late mid-trimester, CT may be discussed and usefully contribute to diagnosis and prognosis assessment. PMID:26670660

  6. Se busca reducir de forma drástica la exposición a la radiación en los exámenes por imágenes

    Cancer.gov

    La exposición a la radiación proveniente de exámenes médicos por imaginología en los Estados Unidos ha aumentado en forma significativa. El NCI y diversas organizaciones patrocinaron una reunión en 2011 para promover esfuerzos para reducir la exposición a la radiación por imaginología.

  7. XXIII Congresso Brasileiro em Engenharia Biomdica XXIII CBEB ANLISE MULTIDIMENSIONAL DA FADIGA MUSCULAR POR MEIO DE

    E-print Network

    Carvalho, João Luiz

    FADIGA MUSCULAR POR MEIO DE ELETROMIOGRAFIA DE SUPERFÍCIE DE ALTA DENSIDADE G. M. Q. Mendonça1 , F. A fadiga muscular, por meio de mapas topológicos (no plano espacial) e regressão linear (no eixo do tempo trabalho propõe a avaliação da fatigabilidade em três momentos -- início, meio e fim -- do esforço. Esta

  8. Alterando o Grau de Colaborao de uma Ferramenta de Compartilhamento de Desktop por Meio de Plugins

    E-print Network

    Barbosa, Alberto

    140 Alterando o Grau de Colaboração de uma Ferramenta de Compartilhamento de Desktop por Meio de colaboração pode ser realizado por meio da interação entre mecanismos de comunicação, coordenação e cooperação

  9. Minerao de Sries Temporais por meio da Extrao de Caractersticas e da Identificao de Motifs

    E-print Network

    Batista, Gustavo

    Mineração de Séries Temporais por meio da Extração de Características e da Identificação de Motifs conhecimento de sé- ries temporais, por meio da extração de características e da identificação de motifs

  10. Astronomy in the Classroom: Why? (Spanish Title: Astronomía en la Clase: ¿Por Qué?) Astronomia na Sala de Aula: Por Quê?

    NASA Astrophysics Data System (ADS)

    Daros Gama, Leandro; Bagdonas Henrique, Alexandre

    2010-07-01

    There are many discussions about the relevance of the topics covered in classes. One subject in particular is the focus of this essay: astronomy. In what sense and to what extent it would be worth to teach it in science or other kind of classes? In this paper we discuss some aspects of the advantages of dealing with this area of knowledge in schools, taking into account the epistemological and axiological dimensions of astronomy, in light of the vision of science as an intelligent dialogue with the world (Bachelard), in addition to the "problematization" knowledge of Paulo Freire. We propose that in fact the Astronomy does not need to be seen as just a new set of contents to be taught, but appears as a set of motivational contents for historical-philosophical discussions, and permit the discussion of concepts of other disciplines. Numerosas discusiones se están llevando a cabo acerca de la pertinencia de los temas tradicionalmente tratados en las clases. Uno de los temas, en particular, es el foco de este ensayo: la astronomía. ¿En qué sentido y en qué medida sería conveniente tratarla en clase, ya sea en clases de ciencias naturales, específicamente en las de astronomía o asignaturas afines? Elaboramos en este artículo algunos aspectos de las ventajas de tratar esta área del conocimiento en las escuelas, teniendo en cuenta las dimensiones epistemológica y axiológica de la astronomía, a la luz de la visión de la ciencia como un diálogo inteligente con el mundo (Bachelard), además de la propuesta del conocimiento "problematizador" de Paulo Freire. Proponemos que en realidad la astronomía no tiene por qué ser vista sólo como un nuevo conjunto de contenidos que se enseñan, sino que aparece como un conjunto de temas de motivación para el debate histórico-filosófico y para permitir la discusión de los conceptos típicos de otras disciplinas. Muitas discussões vêm acontecendo sobre a relevância dos temas abordados em sala de aula. Um tema, em particular, é foco deste ensaio: a astronomia. Em que sentido e em que medida valeria a pena abordá-la em aulas, seja de ciências, seja de astronomia especificamente, ou em disciplinas afins? Discutimos neste texto alguns aspectos das vantagens de se tratar essa área do conhecimento nas escolas, levando em conta as dimensões epistemológica e axiológica da astronomia, à luz da visão da ciência como um diálogo inteligente com o mundo, de Bachelard, além da proposta de problematização do conhecimento, de Paulo Freire. Proporemos que de fato a astronomia não precisa ser vista como apenas um novo conjunto de conteúdos a serem ensinados, mas figura como conjunto de temas motivadores para discussões histórico-filosóficas, além de permitir a abordagem de conceitos típicos de outras disciplinas.

  11. Diario de Sevilla (17-12-2004) POR QU APOYO LA ALTERNANCIA

    E-print Network

    Bilbao Arrese, Jesús Mario

    ciudadanos vivos. Esta visión estratégica de la política como continuación de la guerra por otros medios mantienen algunos socialistas dependen de su posesión del poder. Por ello, buscan mantenerlo a cualquier precio y el precio que tenemos que pagar los andaluces es la baja calidad de nuestro sistema de poder

  12. DISTRIBUCION DE PERSONAL INVESTIGADOR DE PLANTILLA por reas cientficas, escalas y sexo.

    E-print Network

    Fitze, Patrick

    DISTRIBUCION DE PERSONAL INVESTIGADOR DE PLANTILLA DEL CSIC por áreas científicas, escalas y sexo (totales por escla y sexo) Hombres Mujeres Total PI 373 68 441 IC 411 166 577 CT 819 515 1334 Total 1610 Científicas y sexos 16% 17% 35% 22% 7% 3% PI H IC H CT H CT D IC D PI D H O M B R E S M U J E R E S Hombres 23

  13. An investigation of exploitation versus exploration in GBEA optimization of PORS 15 and 16 Problems

    SciTech Connect

    Koch, Kaelynn

    2012-05-08

    It was hypothesized that the variations in time to solution are driven by the competing mechanisms of exploration and exploitation.This thesis explores this hypothesis by examining two contrasting problems that embody the hypothesized tradeoff between exploration and exploitation. Plus one recall store (PORS) is an optimization problem based on the idea of a simple calculator with four buttons: plus, one, store, and recall. Integer addition and store are classified as operations, and one and memory recall are classified as terminals. The goal is to arrange a fixed number of keystrokes in a way that maximizes the numerical result. PORS 15 (15 keystrokes) represents the subset of difficult PORS problems and PORS 16 (16 keystrokes) represents the subset of PORS problems that are easiest to optimize. The goal of this work is to examine the tradeoff between exploitation and exploration in graph based evolutionary algorithm (GBEA) optimization. To do this, computational experiments are used to examine how solutions evolve in PORS 15 and 16 problems when solved using GBEAs. The experiment is comprised of three components; the graphs and the population, the evolutionary algorithm rule set, and the example problems. The complete, hypercube, and cycle graphs were used for this experiment. A fixed population size was used.

  14. Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB

    SciTech Connect

    Tanabe, Mikio; Nimigean, Crina M.; Iverson, T.M.

    2010-06-25

    PorB is the second most prevalent outer membrane protein in Neisseria meningitidis. PorB is required for neisserial pathogenesis and can elicit a Toll-like receptor mediated host immune response. Here, the x-ray crystal structure of PorB has been determined to 2.3 {angstrom} resolution. Structural analysis and cocrystallization studies identify three putative solute translocation pathways through the channel pore: One pathway transports anions nonselectively, one transports cations nonselectively, and one facilitates the specific uptake of sugars. During infection, PorB likely binds host mitochondrial ATP, and cocrystallization with the ATP analog AMP-PNP suggests that binding of nucleotides regulates these translocation pathways both by partial occlusion of the pore and by restricting the motion of a putative voltage gating loop. PorB is located on the surface of N. meningitidis and can be recognized by receptors of the host innate immune system. Features of PorB suggest that Toll-like receptor mediated recognition outer membrane proteins may be initiated with a nonspecific electrostatic attraction.

  15. Nasopharyngeal flora in children with acute otitis media before and after implementation of 7 valent pneumococcal conjugate vaccine in France

    PubMed Central

    2012-01-01

    Background Several studies have investigated the impact of 7-valent pneumococcal conjugate vaccine (PCV7) on pneumococcal (Sp) and staphylococcal (Sa) nasopharyngeal (NP) carriage. Few have investigated the impact on Haemophilus influenzae (Hi) and Moraxella catarrhalis (Mc) carriage. We aimed to compare the NP carriage rates in young children with acute otitis media (AOM) before and after PCV7 implementation in France. Methods Prior to PCV7 implementation, we performed 4 successive randomized trials with NP samples. These studies compared several antibiotic regimens for treating AOM in young children (6 to 30 months). After PCV7 implementation, to assess the impact of the vaccination program on NP flora, young children with AOM were enrolled in a prospective surveillance study. In each study, we obtained an NP sample to analyze the carriage rates of Sp, Hi, Mc and Sa and the factors influencing the carriage. Standardized history and physical examination findings were recorded; the methods used for NP swabs (sampling and cultures) were the same in all studies. Results We enrolled 4,405 children (mean age 13.9 months, median 12.8). Among the 2,598 children enrolled after PCV7 implementation, 98.3% were vaccinated with PCV7. In comparing the pre- and post-PCV7 periods, we found a slight but non-significant decrease in carriage rates of pneumococcus (AOR = 0.85 [0.69;1.05]), H. influenzae (AOR = 0.89 [0.73;1.09]) and S. aureus (AOR = 0.92 [0.70;1.19]). By contrast, the carriage rate of M. catarrhalis increased slightly but not significantly between the 2 periods (AOR = 1.08 [0.95;1.2]). Among Sp carriers, the proportion of PCV7 vaccine types decreased from 66.6% to 10.7% (P < 0.001), penicillin intermediate-resistant strains increased from 30.3% to 43.4% (P < 0.001), and penicillin-resistant strains decreased greatly from 22.8% to 3.8% (P < 0.001). The proportion of Hi ß-lactamase-producing strains decreased from 38.6% to 17.1% (P < 0.001). Conclusion The carriage rates of otopathogen species (Sp, Hi, Mc) and Sa did not significantly change in children with AOM after PCV7 implementation in France. However, we observed significant changes in carriage rates of PCV7 vaccine serotypes and penicillin non-susceptible Sp. PMID:22397629

  16. Evaluation of swabbing methods for estimating the prevalence of bacterial carriage in the upper respiratory tract: a cross sectional study

    PubMed Central

    Coughtrie, A L; Whittaker, R N; Begum, N; Anderson, R; Tuck, A; Faust, S N; Jefferies, J M; Yuen, H M; Roderick, P J; Mullee, M A; Moore, M V; Clarke, S C

    2014-01-01

    Objectives Bacterial carriage in the upper respiratory tract is usually asymptomatic but can lead to respiratory tract infection (RTI), meningitis and septicaemia. We aimed to provide a baseline measure of Streptococcus pneumoniae, Moraxella catarrhalis, Pseudomonas aeruginosa, Staphylococcus aureus, Haemophilus influenzae and Neisseria meningitidis carriage within the community. Self-swabbing and healthcare professional (HCP) swabbing were compared. Design Cross-sectional study. Setting Individuals registered at 20 general practitioner practices within the Wessex Primary Care Research Network South West, UK. Participants 10?448 individuals were invited to participate; 5394 within a self-swabbing group and 5054 within a HCP swabbing group. Self-swabbing invitees included 2405 individuals aged 0–4?years and 3349 individuals aged ?5?years. HCP swabbing invitees included 1908 individuals aged 0–4?years and 3146 individuals aged ?5?years. Results 1574 (15.1%) individuals participated, 1260 (23.4%, 95% CI 22.3% to 24.5%) undertaking self-swabbing and 314 (6.2%, 95% CI 5.5% to 6.9%) undertaking HCP-led swabbing. Participation was lower in young children and more deprived practice locations. Swab positivity rates were 34.8% (95% CI 32.2% to 37.4%) for self-taken nose swabs (NS), 19% (95% CI 16.8% to 21.2%) for self-taken whole mouth swabs (WMS), 25.2% (95% CI 20.4% to 30%) for nasopharyngeal swabs (NPS) and 33.4% (95% CI 28.2% to 38.6%) for HCP-taken WMS. Carriage rates of S. aureus were highest in NS (21.3%). S. pneumoniae carriage was highest in NS (11%) and NPS (7.4%). M. catarrhalis carriage was highest in HCP-taken WMS (28.8%). H. influenzae and P. aeruginosa carriage were similar between swab types. N. meningitidis was not detected in any swab. Age and recent RTI affected carriage of S. pneumoniae and H. influenzae. Participant costs were lower for self-swabbing (£41.21) versus HCP swabbing (£69.66). Conclusions Higher participation and lower costs of self-swabbing as well as sensitivity of self-swabbing favour this method for use in large population-based respiratory carriage studies. PMID:25358677

  17. Longitudinal Nasopharyngeal Carriage and Antibiotic Resistance of Respiratory Bacteria in Indigenous Australian and Alaska Native Children with Bronchiectasis

    PubMed Central

    Hare, Kim M.; Singleton, Rosalyn J.; Grimwood, Keith; Valery, Patricia C.; Cheng, Allen C.; Morris, Peter S.; Leach, Amanda J.; Smith-Vaughan, Heidi C.; Chatfield, Mark; Redding, Greg; Reasonover, Alisa L.; McCallum, Gabrielle B.; Chikoyak, Lori; McDonald, Malcolm I.; Brown, Ngiare; Torzillo, Paul J.; Chang, Anne B.

    2013-01-01

    Background Indigenous children in Australia and Alaska have very high rates of chronic suppurative lung disease (CSLD)/bronchiectasis. Antibiotics, including frequent or long-term azithromycin in Australia and short-term beta-lactam therapy in both countries, are often prescribed to treat these patients. In the Bronchiectasis Observational Study we examined over several years the nasopharyngeal carriage and antibiotic resistance of respiratory bacteria in these two PCV7-vaccinated populations. Methods Indigenous children aged 0.5–8.9 years with CSLD/bronchiectasis from remote Australia (n?=?79) and Alaska (n?=?41) were enrolled in a prospective cohort study during 2004–8. At scheduled study visits until 2010 antibiotic use in the preceding 2-weeks was recorded and nasopharyngeal swabs collected for culture and antimicrobial susceptibility testing. Analysis of respiratory bacterial carriage and antibiotic resistance was by baseline and final swabs, and total swabs by year. Results Streptococcus pneumoniae carriage changed little over time. In contrast, carriage of Haemophilus influenzae declined and Staphylococcus aureus increased (from 0% in 2005–6 to 23% in 2010 in Alaskan children); these changes were associated with increasing age. Moraxella catarrhalis carriage declined significantly in Australian, but not Alaskan, children (from 64% in 2004–6 to 11% in 2010). While beta-lactam antibiotic use was similar in the two cohorts, Australian children received more azithromycin. Macrolide resistance was significantly higher in Australian compared to Alaskan children, while H. influenzae beta-lactam resistance was higher in Alaskan children. Azithromycin use coincided significantly with reduced carriage of S. pneumoniae, H. influenzae and M. catarrhalis, but increased carriage of S. aureus and macrolide-resistant strains of S. pneumoniae and S. aureus (proportion of carriers and all swabs), in a ‘cumulative dose-response’ relationship. Conclusions Over time, similar (possibly age-related) changes in nasopharyngeal bacterial carriage were observed in Australian and Alaskan children with CSLD/bronchiectasis. However, there were also significant frequency-dependent differences in carriage and antibiotic resistance that coincided with azithromycin use. PMID:23940582

  18. Por Secretion System-Dependent Secretion and Glycosylation of Porphyromonas gingivalis Hemin-Binding Protein 35

    PubMed Central

    Shoji, Mikio; Sato, Keiko; Yukitake, Hideharu; Kondo, Yoshio; Narita, Yuka; Kadowaki, Tomoko; Naito, Mariko; Nakayama, Koji

    2011-01-01

    The anaerobic Gram-negative bacterium Porphyromonas gingivalis is a major pathogen in severe forms of periodontal disease and refractory periapical perodontitis. We have recently found that P. gingivalis has a novel secretion system named the Por secretion system (PorSS), which is responsible for secretion of major extracellular proteinases, Arg-gingipains (Rgps) and Lys-gingipain. These proteinases contain conserved C-terminal domains (CTDs) in their C-termini. Hemin-binding protein 35 (HBP35), which is one of the outer membrane proteins of P. gingivalis and contributes to its haem utilization, also contains a CTD, suggesting that HBP35 is translocated to the cell surface via the PorSS. In this study, immunoblot analysis of P. gingivalis mutants deficient in the PorSS or in the biosynthesis of anionic polysaccharide-lipopolysaccharide (A-LPS) revealed that HBP35 is translocated to the cell surface via the PorSS and is glycosylated with A-LPS. From deletion analysis with a GFP-CTD[HBP35] green fluorescent protein fusion, the C-terminal 22 amino acid residues of CTD[HBP35] were found to be required for cell surface translocation and glycosylation. The GFP-CTD fusion study also revealed that the CTDs of CPG70, peptidylarginine deiminase, P27 and RgpB play roles in PorSS-dependent translocation and glycosylation. However, CTD-region peptides were not found in samples of glycosylated HBP35 protein by peptide map fingerprinting analysis, and antibodies against CTD-regions peptides did not react with glycosylated HBP35 protein. These results suggest both that the CTD region functions as a recognition signal for the PorSS and that glycosylation of CTD proteins occurs after removal of the CTD region. Rabbits were used for making antisera against bacterial proteins in this study. PMID:21731719

  19. AMPEROMETRIC MICROBIAL BIOSENSOR FOR DIRECT DETERMINATION OF ORGANOPHOSPHATE NERVE AGENTS USING RECOMBINANT MORAXELLA SP. WITH SURFACE EXPRESSED ORGANOPHOSPHORUS HYDROLASE. (R828160)

    EPA Science Inventory

    The perspectives, information and conclusions conveyed in research project abstracts, progress reports, final reports, journal abstracts and journal publications convey the viewpoints of the principal investigator and may not represent the views and policies of ORD and EPA. Concl...

  20. Informe a la nación indica que los índices de muertes por cáncer siguen bajando

    Cancer.gov

    Los índices de mortalidad por todos los cánceres combinados para hombres, mujeres y niños siguieron bajando en Estados Unidos entre 2004 y 2008, según el Informe Anual a la Nación sobre el Estado del Cáncer de 1975 a 2008. El índice general de diagnóstico

  1. Tarea: Temas Selectos de Estadistica Por entregar el 13 de marzo;

    E-print Network

    Van Horebeek, Johan

    1 Tarea: Temas Selectos de Estad´istica Por entregar el 13 de marzo; 1. Este ejercicio es sobre la para este hip´otesis. Usa una estad´istica de prueba basada en ra- zones de verosimilitud para calcular muestra, n, es suficientemente grande, pode- mos suponer que nuestras estad´isticas de distancia (power

  2. INTRODUCCIN La extraccin de hidrocarburos no convencionales por medio de la tcnica

    E-print Network

    Escolano, Francisco

    fractura hidráulica o fracking está despertando en el mundo entero, y también en España, una considerable: deje@ua.es SEGUIMIENTO POR INTERNET Y MÁS INFORMACIÓN EN http://web.ua.es/es/fracking DIRECCIÓN Y JORNADAS INTERNACIONALES DERECHO Y FRACKING Fundamentos científico-técnicos y económicos -Salvador Ordóñez

  3. No vamos ser devorados por um buraco negro p26/27 Pode estar descansado

    E-print Network

    Instituto de Sistemas e Robotica

    Não vamos ser devorados por um buraco negro p26/27 #12;Pode estar descansado: nâo vamos ser devorados -'buraco negroTrês cientistas em Lisboa quiseram ver se todos os astros acabam um dia em buracos negros, objectos tão densos e maciços que atraem tudo o que está à volta. Para tal, fizeram cálculos num

  4. Enverdceta! Cambia a limpiadores verdes por la salud de los trabajadores y ambiente ms seguro

    E-print Network

    Oliver, Douglas L.

    ¡Enverdéceta! Cambia a limpiadores verdes por la salud de los trabajadores y ambiente más seguro irriten nuestra respiración y puden causar daño a nuestra salud. Estos químicos frecuentemente no ayudan Departamento de Comunicación y el Departamento del Ambiente, Salud y Seguridad de tu institución para elaborar

  5. Armazenar energia mais rapidamente O armazenamento de energia um desafio tecnolgico ainda por vencer. Os supercondensadores

    E-print Network

    Instituto de Sistemas e Robotica

    Armazenar energia mais rapidamente O armazenamento de energia é um desafio tecnológico ainda por: armazena muita energia e de forma muito rápida. Além de ser fabricado através de um processo sim- ples, com de supercondensadores com elevada densidade de energia e potência, o que vem resolver a desvantagem

  6. Prevalencia y tamizaje del Trastorno por Déficit de Atención con Hiperactividad en Costa Rica

    PubMed Central

    Weiss, Nicholas T.; Schuler, Jovita; Monge, Silvia; McGough, James J.; Chavira, Denise; Bagnarello, Monica; Herrera, Luis Diego; Mathews, Carol A.

    2015-01-01

    Resumen La investigación tuvo como propósito estimar la prevalencia del Trastorno por Déficit de Atención con Hiperactividad (TDAH) en Costa Rica y determinar si la versión en español del cuestionario Swanson Nolan and Pelham Scale IV (SNAP-IV) es un instrumento de tamizaje útil en una población de niños y niñas escolares costarricenses. El instrumento fue entregado a padres y maestros de 425 niños entre 5 y 13 años de edad (promedio = 8.8). Todos fueron evaluados con el instrumento Swanson, Kotkin, Agler, M-Flynn and Pelham Scale (SKAMP). Su diagnóstico fue confirmado con una entrevista clínica. La sensibilidad y la especificidad del SNAP-IV fueron evaluadas como predictores de criterios de diagnóstico según el DSM-IV. La prevalencia puntual en la muestra del TDAH fue del 5%. El tamizaje más preciso lo hizo el SNAP-IV completado por el maestro en un corte de 20%, con una sensibilidad de 96% y una especificidad de un 82%. La sensibilidad de los instrumentos completados por los padres fue más baja que aquella de los maestros. El SNAP-IV completado por las maestras con un corte aislando el 20% de los mayores puntajes categorizó correctamente a un 87% de los sujetos. PMID:22432094

  7. The Acquisition of Lexical Meaning in a Study Abroad Context: The Spanish Prepositions "por" and "para."

    ERIC Educational Resources Information Center

    Lafford, Barbara A.; Ryan, John M.

    1995-01-01

    Examination of the development of form/function relations of the prepositions "por" and "para" at different levels of proficiency in the interlanguage of study-abroad students in Granada, Spain, revealed "noncanonical" as well as "canonical" uses of these prepositions. The most common noncanonical uses were as substitutions for other prepositions…

  8. Introduccin Unidad 1 La igualdad de oportunidades se opone a la discriminacin por razn de sexo

    E-print Network

    Fernández Pascual, Ricardo

    sexo 1. Hombres y mujeres: diferentes pero iguales 2. La igualdad legal no implica la igualdad real 3 desapa- rezca la discriminación por razón de sexo, es imprescindible no solamente para la evolución de sexo sobre todo porque ya no es posible creer que las desigualdades son `normales

  9. Ministrio quer matricular 5 mil alunos por ano em cursos curtos

    E-print Network

    Instituto de Sistemas e Robotica

    Ministério quer matricular 5 mil alunos por ano em cursos curtos Ensino superior. Cursos de dois) estima que os novos cursos superiores especializados (CES), de curta duração, "possam ter uma procura de - o investimento nestes cursos de- verá ser da ordem dos 15, 7 mi- lhões de euros, no primeiro ano

  10. Global Microlending in Education Reform: Enseñá Por Argentina and the Neoliberalization of the Grassroots

    ERIC Educational Resources Information Center

    Friedrich, Daniel S.

    2010-01-01

    This article examines the workings and underlying assumptions behind Enseñá por Argentina (Teach for Argentina), one specific program that takes part in the larger and expanding network of Teach for All, by thinking about the ways in which a global push for redefining teaching and teacher education encounters local characteristics and histories,…

  11. Strategies developed by bacteria and virus for protection from the human complement system.

    PubMed

    Blom, A M

    2004-01-01

    The complement system is an important part of innate immunity providing immediate protection against pathogens without a need for previous exposure. Its importance is clearly shown by the fact that patients lacking complement components suffer from fulminant and recurring infections. Complement is an explosive cascade, and in order to control it there are inhibitors present on every human cell and also circulating in blood. However, many infectious agents have developed strategies to prevent clearance and destruction by complement. Some pathogens simply hijack the host's complement inhibitors, while others are able to produce their own homologues of human inhibitors. Knowledge of these mechanisms on a molecular level may aid development of vaccines and novel therapeutic strategies that would be more specific than the use of antibiotics that, apart from causing resistance problems, also affect the normal flora, the outcome of which could be devastating. In this study the structural requirements and functional consequences of interactions between the major soluble inhibitor of complement C4b-binding protein and Neisseria gonorrhoeae, Bordetella pertussis, Streptococcus pyogenes, Escherichia coli K1, Moraxella catarrhalis and Candida albicans are described. Furthermore, a novel inhibitor produced by Kaposi's sarcoma-associated herpesvirus is identified and characterized in detail: KCP. It is shown that KCP inhibits classical C3-convertase and presents activated complement factors C4b and C3b for destruction by a serine proteinase, factor I. Using molecular modelling and site-directed mutagenesis, it was possible to localize sites on the surface of KCP required for complement inhibition and it is concluded that KCP uses molecular mechanisms identical to human inhibitors. PMID:15276914

  12. Panel 6: Vaccines

    PubMed Central

    Pelton, Stephen I.; Pettigrew, Melinda M.; Barenkamp, Stephen J.; Godfroid, Fabrice; Grijalva, Carlos G.; Leach, Amanda; Patel, Janak; Murphy, Timothy F.; Selak, Sanja; Bakaletz, Lauren O.

    2014-01-01

    Objective To update progress on the effectiveness of vaccine for prevention of acute otitis media (AOM) and identification of promising candidate antigens against Streptococcus pneumoniae, nontypeable Haemophilus influenzae, and Moraxella catarrhalis. Review Methods Literature searches were performed in OvidSP and PubMed restricted to articles published between June 2007 and September 2011. Search terms included otitis media, vaccines, vaccine antigens, and each of the otitis pathogens and candidate antigens identified in the ninth conference report. Conclusions The current report provides further evidence for the effectiveness of pneumococcal conjugate vaccines (PCVs) in the prevention of otitis media. Observational studies demonstrate a greater decline in AOM episodes than reported in clinical efficacy trials. Unmet challenges include extending protection to additional serotypes and additional pathogens, the need to prevent early episodes, the development of correlates of protection for protein antigens, and the need to define where an otitis media vaccine strategy fits with priorities for child health. Implications for Practice Acute otitis media continues to be a burden on children and families, especially those who suffer from frequent recurrences. The 7-valent PCV (PCV7) has reduced the burden of disease as well as shifted the pneumococcal serotypes and the distribution of otopathogens currently reported in children with AOM. Antibiotic resistance remains an ongoing challenge. Multiple candidate antigens have demonstrated the necessary requirements of conservation, surface exposure, immunogenicity, and protection in animal models. Further research on the role of each antigen in pathogenesis, in the development of correlates of protection in animal models, and in new adjuvants to elicit responses in the youngest infants is likely to be productive and permit more antigens to move into human clinical trials. PMID:23536534

  13. Ceftaroline in the management of complicated skin and soft tissue infections and community acquired pneumonia

    PubMed Central

    Mpenge, Mbiye A; MacGowan, Alasdair P

    2015-01-01

    Ceftaroline is a new parenteral cephalosporin approved by the European Medicines Agency (EMA) and the US Food and Drug Administration (FDA) for the treatment of complicated skin and soft tissue infections (cSSTIs) including those due to methicillin-resistant Staphylococcus aureus (MRSA), and community-acquired pneumonia (CAP). Ceftaroline has broad-spectrum activity against gram-positive and gram-negative bacteria and exerts its bactericidal effects by binding to penicillin-binding proteins (PBPs), resulting in inhibition of bacterial cell wall synthesis. It binds to PBP 2a of MRSA with high affinity and also binds to all six PBPs in Streptococcus pneumoniae. In in vitro studies, ceftaroline demonstrated potent activity against Staphylococcus aureus (including MRSA and vancomycin-intermediate isolates), Streptococcus pneumoniae (including multidrug resistant isolates), Haemophilus influenzae, Moraxella catarrhalis, and many common gram-negative pathogens, excluding extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae and Pseudomonas aeruginosa. In Phase II and Phase III clinical trials, ceftaroline was noninferior to its comparator agents and demonstrated high clinical cure rates in the treatment of cSSTIs and CAP. It demonstrated favorable outcomes in patients treated for both regulatory-approved indications and unlicensed indications in a retrospective analysis. Ceftaroline is a safe and effective option for treatment in specific patient populations in which its efficacy and safety have been proven. This article reviews the challenges in the treatment of cSSTI and CAP, ceftaroline and its microbiology, pharmacology, efficacy, and safety data which support its use in treatment of cSSTIs and CAP. PMID:25897241

  14. Nonantibiotic macrolides prevent human neutrophil elastase-induced mucus stasis and airway surface liquid volume depletion

    PubMed Central

    Sabater, Juan R.; Clarke, Tainya C.; Tan, Chong D.; Davies, Catrin M.; Liu, Jia; Yeung, Arthur; Garland, Alaina L.; Stutts, M. Jackson; Abraham, William M.; Phillips, Gary; Baker, William R.; Wright, Clifford D.; Wilbert, Sibylle

    2013-01-01

    Mucus clearance is an important component of the lung's innate defense system. A failure of this system brought on by mucus dehydration is common to both cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). Mucus clearance rates are regulated by the volume of airway surface liquid (ASL) and by ciliary beat frequency (CBF). Chronic treatment with macrolide antibiotics is known to be beneficial to both CF and COPD patients. However, chronic macrolide usage may induce bacterial resistance. We have developed a novel macrolide, 2?-desoxy-9-(S)-erythromycylamine (GS-459755), that has significantly diminished antibiotic activity against Staphylococcus aureus, Streptococcus pneumonia, Moraxella catarrhalis, and Haemophilus influenzae. Since neutrophilia frequently occurs in chronic lung disease and human neutrophil elastase (HNE) induces mucus stasis by activating the epithelial sodium channel (ENaC), we tested the ability of GS-459755 to protect against HNE-induced mucus stasis. GS-459755 had no effect on HNE activity. However, GS-459755 pretreatment protected against HNE-induced ASL volume depletion in human bronchial epithelial cells (HBECs). The effect of GS-459755 on ASL volume was dose dependent (IC50 ?3.9 ?M) and comparable to the antibacterial macrolide azithromycin (IC50 ?2.4 ?M). Macrolides had no significant effect on CBF or on transepithelial water permeability. However, the amiloride-sensitive transepithelial voltage, a marker of ENaC activity, was diminished by macrolide pretreatment. We conclude that GS-459755 may limit HNE-induced activation of ENaC and may be useful for the treatment of mucus dehydration in CF and COPD without inducing bacterial resistance. PMID:23542952

  15. Nationwide surveillance of 6 otorhinolaryngological infectious diseases and antimicrobial susceptibility pattern in the isolated pathogens in Japan.

    PubMed

    Suzuki, Kenji; Kurono, Yuichi; Ikeda, Katsuhisa; Watanabe, Akira; Iwamoto, Aikichi; Totsuka, Kyoichi; Kaku, Mitsuo; Iwata, Satoshi; Kadota, Jun-ichi; Hanaki, Hideaki

    2015-07-01

    The Japanese Three Academic Societies Joint Antimicrobial Susceptibility Surveillance Committee has conducted a nationwide surveillance on antimicrobial susceptibility patterns and rates of isolation in 6 otolaryngological diseases. The surveillance program was conducted in the otorhinolaryngological departments of 29 universities, and their 26 affiliated hospitals. Patients suffering from acute otitis media, chronic otitis media, acute nasal sinusitis, chronic nasal sinusitis, acute tonsillitis, and peritonsillar abscess between January 2011 and June 2012 were investigated. The collected swab or incision samples were cultivated for microbial identification, and the drug susceptibility of detected bacteria was measured at the Kitasato University Research Center for Infections and Antimicrobials. The surveillance focused on three gram-positive bacteria (Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus), three gram-negative bacteria (Haemophilus influenzae, Moraxella Catarrhalis, and Pseudomonas aeruginosa), and three anaerobic bacteria (Peptostreptococcus spp., Prevotella spp., and Fusobacterium spp.). Bacterial susceptibility to 39 antimicrobial drugs was investigated. We compared bacterial isolation ratio of each disease in this surveillance from those of past 4 times surveillance which we performed formerly, and we also compared percentage of main drug resistant strains from those of past 4 times surveillance. The age composition between this time and former surveillances was not statistically significant by student-t test. We were unable to completely resolve the rise in resistant bacteria, such as methicillin-resistant S. aureus, penicillin-resistant S. pneumoniae, penicillin-intermediate resistant S. pneumoniae, beta-lactamase non-producing ampicillin-resistant H. influenzae, beta-lactamase producing ampicillin-resistant H. influenzae, and beta-lactamase producing amoxicillin clavulanic acid-resistant H. influenzae. We suggest promoting the proper usage of antimicrobial drugs in order to avoid the spread of these bacteria. PMID:26004175

  16. Nasopharyngeal carriage and macrolide resistance in Indigenous children with bronchiectasis randomized to long-term azithromycin or placebo.

    PubMed

    Hare, K M; Grimwood, K; Chang, A B; Chatfield, M D; Valery, P C; Leach, A J; Smith-Vaughan, H C; Morris, P S; Byrnes, C A; Torzillo, P J; Cheng, A C

    2015-11-01

    Although long-term azithromycin decreases exacerbation frequency in bronchiectasis, increased macrolide resistance is concerning. We investigated macrolide resistance determinants in a secondary analysis of a multicenter randomized controlled trial. Indigenous Australian children living in remote regions and urban New Zealand M?ori and Pacific Islander children with bronchiectasis were randomized to weekly azithromycin (30 mg/kg) or placebo for up to 24 months and followed post-intervention for up to 12 months. Nurses administered and recorded medications given and collected nasopharyngeal swabs 3-6 monthly for culture and antimicrobial susceptibility testing. Nasopharyngeal carriage of Haemophilus influenzae and Moraxella catarrhalis was significantly lower in azithromycin compared to placebo groups, while macrolide-resistant Streptococcus pneumoniae and Staphylococcus aureus carriage was significantly higher. Australian children, compared to New Zealand children, had higher carriage overall, significantly higher carriage of macrolide-resistant bacteria at baseline (16/38 versus 2/40 children) and during the intervention (69/152 versus 22/239 swabs), and lower mean adherence to study medication (63 % versus 92 %). Adherence ?70 % (versus <70 %) in the Australian azithromycin group was associated with lower carriage of any pathogen [odds ratio (OR) 0.19, 95 % confidence interval (CI) 0.07-0.53] and fewer macrolide-resistant pathogens (OR 0.34, 95 % CI 0.14-0.81). Post-intervention (median 6 months), macrolide resistance in S. pneumoniae declined significantly in the azithromycin group, from 79 % (11/14) to 7 % (1/14) of positive swabs, but S. aureus strains remained 100 % macrolide resistant. Azithromycin treatment, the Australian remote setting, and adherence <70 % were significant independent determinants of macrolide resistance in children with bronchiectasis. Adherence to treatment may limit macrolide resistance by suppressing carriage. PMID:26363637

  17. A longitudinal study of lung bacterial pathogens in patients with primary ciliary dyskinesia.

    PubMed

    Alanin, M C; Nielsen, K G; von Buchwald, C; Skov, M; Aanaes, K; Høiby, N; Johansen, H K

    2015-12-01

    In patients with primary ciliary dyskinesia (PCD), impaired mucociliary clearance leads to an accumulation of secretions in the airways and susceptibility to repeated bacterial infections. The primary aim of this study was to investigate the bacterial flora in non-chronic and chronic infections in the lower airways of patients with PCD. We retrospectively reviewed the presence of bacteria from patients with PCD during an 11-year period and genotyped 35 Pseudomonas aeruginosa isolates from 12 patients with chronic infection using pulsed-field gel electrophoresis. We identified 5450 evaluable cultures from 107 patients with PCD (median age 17 years, range 0-74 years) (median age at diagnosis 7.8 years, range 0-63 years). Haemophilus influenzae was the most frequent microorganism. Other common pathogens were P. aeruginosa, Streptococcus pneumoniae, Moraxella catarrhalis and Staphylococcus aureus. The number of patients colonized with P. aeruginosa at least once varied from 11 to 44 patients (15-47%) annually, and 42 patients (39%) met the criteria for chronic infection at least once. Pseudomonas aeruginosa was more frequently isolated in teenagers and adults than children (p 0.02) and the prevalence was significantly lower in patients with preschool (<6 years) PCD diagnosis (p 0.04). Ten out of 12 patients (83%) were chronically infected with a unique clone-type of P. aeruginosa. No sharing of clone-types or patient-to-patient transmission was observed. In conclusion, PCD patients were infected by a unique set of bacteria acquired in an age-dependent sequence. Pseudomonas aeruginosa frequently colonizes the lower respiratory tract and the incidence of chronic infection was higher than previously reported. PMID:26341913

  18. Development of two real-time multiplex PCR assays for the detection and quantification of eight key bacterial pathogens in lower respiratory tract infections

    PubMed Central

    Gadsby, N.J.; McHugh, M.P.; Russell, C.D.; Mark, H.; Conway Morris, A.; Laurenson, I.F.; Hill, A.T.; Templeton, K.E.

    2015-01-01

    The frequent lack of a positive and timely microbiological diagnosis in patients with lower respiratory tract infection (LRTI) is an important obstacle to antimicrobial stewardship. Patients are typically prescribed broad-spectrum empirical antibiotics while microbiology results are awaited, but, because these are often slow, negative, or inconclusive, de-escalation to narrow-spectrum agents rarely occurs in clinical practice. The aim of this study was to develop and evaluate two multiplex real-time PCR assays for the sensitive detection and accurate quantification of Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Moraxella catarrhalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. We found that all eight bacterial targets could be reliably quantified from sputum specimens down to a concentration of 100 CFUs/reaction (8333 CFUs/mL). Furthermore, all 249 positive control isolates were correctly detected with our assay, demonstrating effectiveness on both reference strains and local clinical isolates. The specificity was 98% on a panel of nearly 100 negative control isolates. Bacterial load was quantified accurately when three bacterial targets were present in mixtures of varying concentrations, mimicking likely clinical scenarios in LRTI. Concordance with culture was 100% for culture-positive sputum specimens, and 90% for bronchoalveolar lavage fluid specimens, and additional culture-negative bacterial infections were detected and quantified. In conclusion, a quantitative molecular test for eight key bacterial causes of LRTI has the potential to provide a more sensitive decision-making tool, closer to the time-point of patient admission than current standard methods. This should facilitate de-escalation from broad-spectrum to narrow-spectrum antibiotics, substantially improving patient management and supporting efforts to curtail inappropriate antibiotic use. PMID:25980353

  19. Nationwide surveillance of bacterial respiratory pathogens conducted by the surveillance committee of Japanese Society of Chemotherapy, the Japanese Association for Infectious Diseases, and the Japanese Society for Clinical Microbiology in 2010: General view of the pathogens' antibacterial susceptibility.

    PubMed

    Yanagihara, Katsunori; Kadota, Junichi; Aoki, Nobuki; Matsumoto, Tetsuya; Yoshida, Masaki; Yagisawa, Morimasa; Oguri, Toyoko; Sato, Junko; Ogasawara, Kazuhiko; Wakamura, Tomotaro; Sunakawa, Keisuke; Watanabe, Akira; Iwata, Satoshi; Kaku, Mitsuo; Hanaki, Hideaki; Ohsaki, Yoshinobu; Watari, Tomohisa; Toyoshima, Eri; Takeuchi, Kenichi; Shiokoshi, Mayumi; Takeda, Hiroaki; Miki, Makoto; Kumagai, Toshio; Nakanowatari, Susumu; Takahashi, Hiroshi; Utagawa, Mutsuko; Nishiya, Hajime; Kawakami, Sayoko; Kobayashi, Nobuyuki; Takasaki, Jin; Mezaki, Kazuhisa; Konosaki, Hisami; Aoki, Yasuko; Yamamoto, Yumiko; Shoji, Michi; Goto, Hajime; Saraya, Takeshi; Kurai, Daisuke; Okazaki, Mitsuhiro; Niki, Yoshihito; Yoshida, Koichiro; Kawana, Akihiko; Saionji, Katsu; Fujikura, Yuji; Miyazawa, Naoki; Kudo, Makoto; Sato, Yoshimi; Yamamoto, Masaki; Yoshida, Takashi; Nakamura, Masahiko; Tsukada, Hiroki; Imai, Yumiko; Tsukada, Ayami; Kawasaki, Satoshi; Honma, Yasuo; Yamamoto, Toshinobu; Ban, Nobuyoshi; Mikamo, Hiroshige; Sawamura, Haruki; Miyara, Takayuki; Toda, Hirofumi; Sato, Kaori; Nakamura, Tadahiro; Fujikawa, Yasunori; Mitsuno, Noriko; Mikasa, Keiichi; Kasahara, Kei; Sano, Reiko; Sugimoto, Keisuke; Asari, Seishi; Nishi, Isao; Toyokawa, Masahiro; Miyashita, Naoyuki; Koguchi, Yutaka; Kusano, Nobuchika; Mihara, Eiichirou; Kuwabara, Masao; Watanabe, Yaeko; Kawasaki, Yuji; Takeda, Kenichi; Tokuyasu, Hirokazu; Masui, Kayoko; Negayama, Kiyoshi; Hiramatsu, Kazufumi; Aoki, Yosuke; Fukuoka, Mami; Magarifuchi, Hiroki; Nagasawa, Zenzo; Suga, Moritaka; Muranaka, Hiroyuki; Morinaga, Yoshitomo; Honda, Junichi; Fujita, Masaki

    2015-06-01

    The nationwide surveillance on antimicrobial susceptibility of bacterial respiratory pathogens from patients in Japan, was conducted by Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases and Japanese Society for Clinical Microbiology in 2010. The isolates were collected from clinical specimens obtained from well-diagnosed adult patients with respiratory tract infections during the period from January and April 2010 by three societies. Antimicrobial susceptibility testing was conducted at the central reference laboratory according to the method recommended by Clinical and Laboratory Standard Institutes using maximum 45 antibacterial agents. Susceptibility testing was evaluable with 954 strains (206 Staphylococcus aureus, 189 Streptococcus pneumoniae, 4 Streptococcus pyogenes, 182 Haemophilus influenzae, 74 Moraxella catarrhalis, 139 Klebsiella pneumoniae and 160 Pseudomonas aeruginosa). Ratio of methicillin-resistant S. aureus was as high as 50.5%, and those of penicillin-intermediate and -resistant S. pneumoniae were 1.1% and 0.0%, respectively. Among H. influenzae, 17.6% of them were found to be ?-lactamase-non-producing ampicillin (ABPC)-intermediately resistant, 33.5% to be ?-lactamase-non-producing ABPC-resistant and 11.0% to be ?-lactamase-producing ABPC-resistant strains. Extended spectrum ?-lactamase-producing K. pneumoniae and multi-drug resistant P. aeruginosa with metallo ?-lactamase were 2.9% and 0.6%, respectively. Continuous national surveillance of antimicrobial susceptibility of respiratory pathogens is crucial in order to monitor changing patterns of susceptibility and to be able to update treatment recommendations on a regular basis. PMID:25817352

  20. In Vitro and In Vivo Profiles of ACH-702, an Isothiazoloquinolone, against Bacterial Pathogens?

    PubMed Central

    Pucci, Michael J.; Podos, Steven D.; Thanassi, Jane A.; Leggio, Melissa J.; Bradbury, Barton J.; Deshpande, Milind

    2011-01-01

    ACH-702, a novel isothiazoloquinolone (ITQ), was assessed for antibacterial activity against a panel of Gram-positive and Gram-negative clinical isolates and found to possess broad-spectrum activity, especially against antibiotic-resistant Gram-positive strains, including methicillin-resistant Staphylococcus aureus (MRSA). For Gram-negative bacteria, ACH-702 showed exceptional potency against Haemophilus influenzae, Moraxella catarrhalis, and a Neisseria sp. but was less active against members of the Enterobacteriaceae. Good antibacterial activity was also evident against several anaerobes as well as Legionella pneumophila and Mycoplasma pneumoniae. Excellent bactericidal activity was observed for ACH-702 against several bacterial pathogens in time-kill assays, and postantibiotic effects (PAEs) of >1 h were evident with both laboratory and clinical strains of staphylococci at 10× MIC and similar in most cases to those observed for moxifloxacin at the same MIC multiple. In vivo efficacy was demonstrated against S. aureus with murine sepsis and thigh infection models, with decreases in the number of CFU/thigh equal to or greater than those observed after vancomycin treatment. Macromolecular synthesis assays showed specific dose-dependent inhibition of DNA replication in staphylococci, and biochemical analyses indicated potent dual inhibition of two essential DNA replication enzymes: DNA gyrase and topoisomerase IV. Additional biological data in support of an effective dual targeting mechanism of action include the following: low MIC values (?0.25 ?g/ml) against staphylococcal strains with single mutations in both gyrA and grlA (parC), retention of good antibacterial activity (MICs of ?0.5 ?g/ml) against staphylococcal strains with two mutations in both gyrA and grlA, and low frequencies for the selection of higher-level resistance (<10?10). These promising initial data support further study of isothiazoloquinolones as potential clinical candidates. PMID:21464250

  1. Diagnóstico diferencial en la encefalitis por anticuerpos contra el receptor NMDA

    PubMed Central

    González-Valcárcel, J.; Rosenfeld, M.R.; Dalmau, J.

    2011-01-01

    Resumen Introducción La encefalitis por anticuerpos contra el receptor de NMDA (NMDAR) suele desarrollarse como un síndrome característico de evolución multifásica y diagnóstico diferencial amplio. Pacientes Presentamos a 2 pacientes diagnosticadas de encefalitis por anticuerpos NMDAR con un cuadro clínico típico, pero que inicialmente señaló otras etiologías. Discusión La afectación frecuente de pacientes jóvenes con manifestaciones psiquiátricas prominentes indica frecuentemente otras consideraciones diagnósticas; las más frecuentes son las encefalitis virales, los procesos psiquiátricos y el síndrome neuroléptico maligno. Varios síndromes previamente definidos de manera parcial o descriptiva en adultos y pacientes pediátricos probablemente eran casos de encefalitis anti-NMDAR. Conclusiones La encefalitis anti-NMDAR debe considerarse en pacientes jóvenes con manifestaciones psiquiátricas subagudas, movimientos anormales y alteraciones autonómicas. La caracterización clínica e inmunológica de esta enfermedad ha llevado a la identificación de nuevos anticuerpos que afectan a procesos de memoria, aprendizaje, conducta y psicosis. PMID:20964986

  2. Aquisição fonológica do português brasileiro por crianças ouvintes bilíngues bimodais e surdas usuárias de implante coclear

    PubMed Central

    Cruz, Carina Rebello; Finger, Ingrid

    2014-01-01

    Resumo O presente estudo investiga a aquisição fonológica do Português Brasileiro (PB) por 24 crianças ouvintes bilíngues bimodais, com acesso irrestrito à Língua Brasileira de Sinais (Libras), e por 6 crianças surdas que utilizam implante coclear (IC), com acesso restrito ou irrestrito à Libras. Para a avaliação do sistema fonológico das crianças em PB, foi utilizada a Parte A, Prova de Nomeação, do ABFW – Teste de Linguagem Infantil (ANDRADE et al. 2004). Os resultados revelaram que as crianças ouvintes bilíngues bimodais e a criança surda usuária de IC com acesso irrestrito à Libras apresentaram processo de aquisição fonológica esperada (normal) para a sua faixa etária. Considera-se que a aquisição precoce e o acesso irrestrito à Libras podem ter sido determinantes para o desempenho dessas crianças no teste oral utilizado. PMID:25506105

  3. MTODOS MULTIGRID PARA A SIMULAO POR ELEMENTOS FINITOS DE ESCOAMENTOS IMISCVEIS EM

    E-print Network

    Coutinho, Alvaro L. G. A.

    1 M�TODOS MULTIGRID PARA A SIMULA��O POR ELEMENTOS FINITOS DE ESCOAMENTOS IMISCÍVEIS EM MEIOS pressão em escoamentos de fluidos imiscíveis em meios porosos. A equação da saturação é aproximada; Meios Porosos. #12; 2 1. INTRODU��O Nos últimos anos os métodos multigrid tem sido largamente estudados

  4. ATP for the portable 500 CFM exhauster POR-006 skid D

    SciTech Connect

    Keller, C.M.

    1997-07-29

    This Acceptance Test Plan is for a 500 CFM Portable Exhauster POR-006 to be used for saltwell pumping. The Portable Exhauster System will be utilized to eliminate potential flammable gases that may exist within the dome space of the tank. This Acceptance Plan will test and verify that the exhauster meets the specified design criteria, safety requirements, operations requirements, and will provide a record of the functional test results.

  5. Análise dos Conceitos Astronômicos Apresentados por Professores de Algumas Escolas Estaduais Brasileiras

    NASA Astrophysics Data System (ADS)

    Voelzke, Marcos Rincon; Gonzaga, Edson Pereira

    2011-12-01

    A razão para o desenvolvimento deste trabalho baseia-se no fato de que muitos professores da Educação Básica (EB) não lidam com conceitos relacionados à astronomia, e quando o fazem eles simplesmente seguem livros didáticos que podem conter erros conceituais. Como é de conhecimento geral a astronomia é um dos conteúdos a serem ensinados na EB fazendo parte dos Parâmetros Curriculares Nacionais e das Propostas Curriculares do Estado de São Paulo, mas é um fato, que vários pesquisadores apontam, a existência de muitos problemas no ensino da astronomia. Com o propósito de minimizar algumas dessas deficiências foi realizado um trabalho de pesquisa com a utilização de questionários pré e pós pesquisa, para tanto foi desenvolvido um Curso de Extensão Universitária para professores da Diretoria de Ensino Regional (DE) que abrange Mauá, Ribeirão Pires e Rio Grande da Serra (no Estado de São Paulo) com os seguintes objetivos: levantar concepções alternativas; subsidiar os professores por meio de palestras, debates e workshops, e verificar o sucesso da aprendizagem após o curso, adotando-se como referência, para a análise dos resultados, os dicionários de Língua Portuguesa (FERREIRA, 2004) e Enciclopédico de Astronomia e Astronáutica (MOUR?O, 1995). Portanto, dezesseis questões foram aplicadas antes e após o curso, assim pode-se verificar após a pesquisa que 100,0% dos professores sabiam os nomes das fases da Lua, 97,0% entenderam que o Sistema Solar é composto por oito planetas, 78,1% foram capazes de explicar como ocorre um eclipse lunar, um eclipse solar e um solstício, 72,7% sabiam como explicar a ocorrência das estações do ano; 64,5% explicaram corretamente a ocorrência do equinócio, 89,7% foram capazes de definir adequadamente o termo cometa; 63,6% definiram asteróide, 54,5% meteoro, 58,1% galáxia, e 42,4% planeta. Os resultados obtidos indicam uma aprendizagem significativa por parte dos participantes.

  6. C ALCULO Y DISE ~NO ASISTIDO POR ORDENADOR DE TOMAS DE TIERRA CON MODELOS DE SUELO NO UNIFORMES

    E-print Network

    Colominas, Ignasi

    y de Representacion Escuela Tecnica Superior de Ingenieros de Caminos, Canales y Puertos Universidad Elementos de Con- torno desarrollada por los autores [4] ha permitido identi#12;car esta familia de metodos

  7. Expression, purification and preliminary X-ray analysis of the Neisseria meningitidis outer membrane protein PorB

    SciTech Connect

    Tanabe, Mikio; Iverson, Tina M.

    2010-01-28

    The Neisseria meningitidis outer membrane protein PorB was expressed in Escherichia coli and purified from inclusion bodies by denaturation in urea followed by refolding in buffered LDAO on a size-exclusion column. PorB has been crystallized in three different crystal forms: C222, R32 and P6{sub 3}. The C222 crystal form may contain either one or two PorB monomers in the asymmetric unit, while both the R32 and P6{sub 3} crystal forms contained one PorB monomer in the asymmetric unit. Of the three, the P6{sub 3} crystal form had the best diffraction quality, yielding data extending to 2.3 {angstrom} resolution.

  8. Una propuesta para el dise~no de m'etodos de Jacobi unilaterales por bloques para el Problema Sim'etrico de Valores Propios \\Lambda

    E-print Network

    Giménez, Domingo

    esta secci'on estudiaremos m'etodos de Jacobi bilaterales con el fin de poder compararlos con los trabaja construyendo una secuencia de matrices fA l g por medio de \\Lambda Parcialmente subvencionado por

  9. Distribucin de los graduados en titulaciones de centros propios en el curso acadmico 2007 / 2008, por centro, plan de estudios y sexo.

    E-print Network

    Autonoma de Madrid, Universidad

    , por centro, plan de estudios y sexo. CENTRO PLAN DE ESTUDIOS TOTAL MUJERES CIENCIAS DIPLOMADO EN académico 2007 / 2008, por centro, plan de estudios y sexo. CENTRO PLAN DE ESTUDIOS TOTAL MUJERES ESC. UNIV

  10. Substrate-specific modulation of CYP3A4 activity by genetic variants of cytochrome P450 oxidoreductase (POR)

    PubMed Central

    Agrawal, Vishal; Choi, Ji Ha; Giacomini, Kathleen M.; Miller, Walter L.

    2010-01-01

    Objectives CYP3A4 receives electrons from P450 oxidoreductase (POR) to metabolize about 50% of clinically used drugs. There is substantial inter-individual variation in CYP3A4 catalytic activity that is not explained by CYP3A4 genetic variants. CYP3A4 is flexible and distensible, permitting it to accommodate substrates varying in shape and size. To elucidate mechanisms of variability in CYP3A4 catalysis, we examined the effects of genetic variants of POR, and explored the possibility that substrate-induced conformational changes in CYP3A4 differentially affect the ability of POR variants to support catalysis. Methods We expressed human CYP3A4 and four POR variants (Q153R, A287P, R457H, A503V) in bacteria, reconstituted them in vitro and measured the Michaelis constant and maximum velocity with testosterone, midazolam, quinidine and erythromycin as substrates. Results POR A287P and R457H had low activity with all substrates; Q153R had 76–94% of wild type (WT) activity with midazolam and erythromycin, but 129–150% activity with testosterone and quinidine. The A503V polymorphism reduced CYP3A4 activity to 61–77% of wild type with testosterone and midazolam, but had nearly wild type activity with quinidine and erythromycin. Conclusion POR variants affect CYP3A4 activities. The impact of a POR variant on catalysis by CYP3A4 is substrate-specific, probably due to substrate-induced conformational changes in CYP3A4. PMID:20697309

  11. Using the PORS Problems to Examine Evolutionary Optimization of Multiscale Systems

    SciTech Connect

    Reinhart, Zachary; Molian, Vaelan; Bryden, Kenneth

    2013-01-01

    Nearly all systems of practical interest are composed of parts assembled across multiple scales. For example, an agrodynamic system is composed of flora and fauna on one scale; soil types, slope, and water runoff on another scale; and management practice and yield on another scale. Or consider an advanced coal-fired power plant: combustion and pollutant formation occurs on one scale, the plant components on another scale, and the overall performance of the power system is measured on another. In spite of this, there are few practical tools for the optimization of multiscale systems. This paper examines multiscale optimization of systems composed of discrete elements using the plus-one-recall-store (PORS) problem as a test case or study problem for multiscale systems. From this study, it is found that by recognizing the constraints and patterns present in discrete multiscale systems, the solution time can be significantly reduced and much more complex problems can be optimized.

  12. Obtención de la curva de luz en la ocultación de 35 Sgr por Júpiter el 6 de marzo de 1996

    NASA Astrophysics Data System (ADS)

    Paolantonio, S.; Duffard, R.; Carranza, G.

    La ocultación de la estrella de quinta magnitud 35 Sgr por Júpiter, se produjo el 6 de Marzo de 1996 a las 13 hs. TU. El objetivo era medir el cambio del flujo de la estrella en el ingreso y egreso por el limbo del planeta. Con estos datos se pueden determinar parámetros físicos del planeta (radio, eccentricidad) y de su atmósfera (escala de altura, temperatura, densidad, presión) Para lograr ésto se programó la cámara CCD TH 7896 1024 x 1025 instalada en el telescopio de 1.54 m de Bosque Alegre con el objetivo de lograr 2 imágenes por segundo. De esta forma se obtuvieron 2100 imágenes de la inmersión y otras tantas de la emersión. Hubo que tener grandes precauciones para evitar la saturación del CCD ya que la observación se realizó de día. En este momento las imágenes se encuentran en el Department of Planetary Sciences, Lunar and Planetary Laboratory, University of Arizona, para su reducción.

  13. Estimaciones de Prevalencia del VIH por Género y Grupo de Riesgo en Tijuana, México: 2006

    PubMed Central

    Iñiguez-Stevens, Esmeralda; Brouwer, Kimberly C.; Hogg, Robert S.; Patterson, Thomas L.; Lozada, Remedios; Magis-Rodriguez, Carlos; Elder, John P.; Viani, Rolando M.; Strathdee, Steffanie A.

    2010-01-01

    OBJETIVO Estimar la prevalencia del VIH en adultos de 15-49 años de edad en Tijuana, México - en la población general y en subgrupos de riesgo en el 2006. METODOS Se obtuvieron datos demográficos del censo Mexicano del 2005, y la prevalencia del VIH se obtuvo de la literatura. Se construyó un modelo de prevalencia del VIH para la población general y de acuerdo al género. El análisis de sensibilidad consistió en estimar errores estándar del promedio-ponderado de la prevalencia del VIH y tomar derivados parciales con respecto a cada parámetro. RESULTADOS La prevalencia del VIH es 0.54%(N = 4,347) (Rango: 0.22%–0.86%, (N = 1,750–6,944)). Esto sugiere que 0.85%(Rango: 0.39%–1.31%) de los hombres y 0.22%(Rango: 0.04%–0.40%) de las mujeres podrían ser VIH-positivos. Los hombres que tienen sexo con hombres (HSH), las trabajadoras sexuales usuarias de drogas inyectables (MTS-UDI), MTS-noUDI, mujeres UDI, y los hombres UDI contribuyeron las proporciones más elevadas de personas infectadas por el VIH. CONCLUSIONES El número de adultos VIH-positivos entre subgrupos de riesgo en la población de Tijuana es considerable, marcando la necesidad de enforcar las intervenciones de prevención en sus necesidades específicas. El presente modelo estima que hasta 1 en cada 116 adultos podrían ser VIH-positivos. PMID:19685824

  14. Derechos Bsicos de Ausencia La Ley de Ausencia Familiar y Mdica (FMLA-por sus siglas en ingls) exige

    E-print Network

    Barr, Maureen

    Derechos Básicos de Ausencia La Ley de Ausencia Familiar y Médica (FMLA-por sus siglas en inglés condición de salud seria que le impida al empleado desempeñar su puesto. Derechos de Ausencia Para Familias avisado de una llamada a estado de servicio activo bajo cobertura pueden usar su derecho de ausencia de 12

  15. Uso de hbitat del jagUar a escala regional en Un paisaje dominado por actividades hUmanas

    E-print Network

    Nacional Autónoma de México, Universidad

    101 Uso de hábitat del jagUar a escala regional en Un paisaje dominado por actividades hUmanas en, fragmentándolas y reduciendo el hábitat disponible para la fauna silvestre. Las poblaciones de jaguar han la Península de Yucatán mantiene la población más numerosa de jaguar en México. La conservación de la

  16. La doctora Amelie Ramírez y la investigación de desigualdades de salud por cáncer en la comunidad la

    Cancer.gov

    La doctora Ramírez es la investigadora principal de Redes en Acción, un centro del programa de redes comunitarias subvencionado por el NCI que se propone reducir la incidencia del cáncer en la comunidad latina a través de una red nacional de grupos comunitarios, investigadores, agencias de salud gubernamentales y la población en general.

  17. Los índices de mortalidad por cáncer de pulmón siguen en descenso y contribuyen a la continua reducc

    Cancer.gov

    El Informe Anual a la Nación sobre el Estado del Cáncer (1975 a 2010), mostró un descenso más acelerado que en años anteriores de los índices de mortalidad por cáncer de pulmón. También contiene una sección especial que destaca los efectos significativos

  18. [Professor Frantisek Por MD and Professor Robert Klopstock MD, students at Budapest and Prague Faculties of Medicine].

    PubMed

    Mydlík, M; Derzsiová, K

    2010-11-01

    Professor Frantisek Por MD and Professor Robert Klopstock MD were contemporaries, both born in 1899, one in Zvolen, the other in Dombovar, at the time of Austro-Hungarian Monarchy. Prof. Por attended the Faculty of Medicine in Budapest from 1918 to 1920, and Prof. Klopstock studied at the same place between 1917 and 1919. From 1920 until graduation on 6th February 1926, Prof. Por continued his studies at the German Faculty of Medicine, Charles University in Prague. Prof. Klopstock had to interrupt his studies in Budapest due to pulmonary tuberculosis; he received treatment at Tatranske Matliare where he befriended Franz Kafka. Later, upon Kafka's encouragement, he changed institutions and continued his studies at the German Faculty of Medicine, Charles University in Prague, where he graduated the first great go. It is very likely that, during their studies in Budapest and Prague, both professors met repeatedly, even though their life paths later separated. Following his graduation, Prof. Por practiced as an internist in Prague, later in Slovakia, and from 1945 in Kosice. In 1961, he was awarded the title of university professor of internal medicine at the Faculty of Medicine, Pavol Jozef Safarik University in Kosice, where he practiced until his death in 1980. Prof. Klopstock continued his studies in Kiel and Berlin. After his graduation in 1933, he practiced in Berlin as a surgeon and in 1938 left for USA. In 1962, he was awarded the title of university professor of pulmonary surgery in NewYork, where he died in 1972. PMID:21250499

  19. FUNDAMENTACIN El taekwondo es un arte marcial de origen coreano que se destaca por la variedad y

    E-print Network

    Escolano, Francisco

    TAEKWONDO FUNDAMENTACIÓN El taekwondo es un arte marcial de origen coreano que se destaca por la millones de personas. OBJETIVOS Los beneficios que se demuestran con la práctica del taekwondo son: 1 SESIONES 1. Calentamiento: Se realizará durante 20 minutos aproximadamente, preparando los músculos y

  20. Functional POR A503V is associated with the risk of bladder cancer in a Chinese population

    PubMed Central

    Xiao, Xue; Ma, Gaoxiang; Li, Shushu; Wang, Meilin; Liu, Nian; Ma, Lan; Zhang, Zhan; Chu, Haiyan; Zhang, Zhengdong; Wang, Shou-Lin

    2015-01-01

    Human cytochrome P450 oxidoreductase (POR) plays important roles in the metabolism of exogenous carcinogens and endogenous sterol hormones. However, few studies have explored the association between POR variants and the risk of bladder cancer. In this study, we first sequenced all 16 POR exons among 50 randomly selected controls, and found three variants, rs1135612, rs1057868 (A503V) and rs2228104, which were then assessed the relation to risk of bladder cancer in a case-control study of 1,050 bladder cancer cases and 1,404 cancer-free controls in a Chinese population. People with A503V TT genotype have a decreased risk of bladder cancer in a recessive model (TT vs. CC/CT, OR?=?0.73, 95% CI?=?0.57–0.93), which was more pronounced among elderly male, non-smoking, subjects. Especially, A503V TT genotype showed a protective effect in the invasive tumor stage. Functional analysis revealed that A503V activity decreased in cytochrome c reduction (50.5 units/mg vs. 135.4 units/mg), mitomycin C clearance (38.3% vs. 96.8%), and mitomycin C-induced colony formation (78.0 vs 34.3 colonies per dish). The results suggested that POR A503V might decrease the risk of bladder cancer by reducing its metabolic activity, and should be a potential biomarker for predicting the susceptibility to human bladder cancer. PMID:26123203

  1. Inversor Resonante de Tres Elementos L-LC con Caracteristica Cortocircuitable para Aplicaciones de Calentamiento por Induccion

    NASA Astrophysics Data System (ADS)

    Espi Huerta, Jose Miguel

    Los generadores de calentamiento por induccion son puentes inversores con carga resonante, cuya mision es basicamente crear una corriente sinusoidal de gran amplitud sobre la "bobina de caldeo", que forma parte del tanque resonante. En el interior de esta bobina se introduce la pieza que se desea calentar. EI campo magnetico creado induce corrientes superficiales (corrientes de Foucault) sobre la pieza, que producen su calentamiento. Los tanques resonantes (tambien llamados osciladores) utilizados en la actualidad son el resonante serie y el resonante paralelo. Aunque ya desde hace algun tiempo se vienen construyendo generadores de alta potencia basados en estos dos osciladores, el exito nunca ha. sido completo en ninguno de los dos casos. Tal y como se explica en la introduccion de esta memoria, los puentes inversores utilizados deben operar sobre una carga inductiva (corriente retrasada) para evitar el fenomeno de la recuperacion inversa de sus diodos y la consiguiente ruptura de los transistores. De la restriccion topologica anterior se deduce que el generador paralelo debe conmutar a frecuencias inferiores a la resonancia, y el serie a frecuencias superiores. A esta restriccion topologica hay que unir otra que es exclusiva del calentamiento por induccion: La corriente por la bobina de caldeo debe ser sinusoidal. De no ser asi, resultaria imposible disponer toda la potencia de calentamiento sobre la pieza en el espesor requerido por la aplicacion. Como consecuencia, los inversores no pueden operar por debajo de la frecuencia de resonancia del oscilador, pues en ese caso se amplifican los armonicos de orden superior de la tension/corriente de entrada situados sobre la resonancia, con la consiguiente distorsion de la corriente de salida. La conjuncion de las dos restricciones anteriores obligan al inversor paralelo a funcionar a la frecuencia de resonancia del oscilador. Esto imposibilita un control por variacion de frecuencia, regulandose la potencia desde la seccion de entrada mediante un mayor o menor aporte de corriente al puente. Como consecuencia, la seccion de entrada del paralelo, ya de por si mas voluminosa que lao del serie por el uso de grandes componentes magneticos (bobinas de filtro o de "alisamiento"), result a tambien mas complicada y costosa debido a la necesidad de ser implementada mediante rectificador controlado. Ademas, la regulacion que ofrece el rectificador es pobre, dada su baja frecuencia de conmutacion. En cambio, el circuito serie puede funcionar por encima de la resonancia manteniendo una secuencia de conmutacion sin riesgos de recuperacion inversa y con una corriente de salida practicamente sinusoidal, lo que permite un control de la potencia por variacion de frecuencia. Puesto que la tarea de regulacion se realiza desde el puente inversor, la regulacion resulta mucho mas eficaz y la seccion de entrada se puede implementar mediante un simple rectificador no controlado y un condensador de filtro. (Abstract shortened by UMI.).

  2. Estudio sobre las dosis de radiación y los riesgos de cáncer causados por la prueba atómica “Trinity” realizada en 1945

    Cancer.gov

    El NCI tiene planeado realizar entrevistas en profundidad para determinar cuál era la alimentación característica de las poblaciones indígenas americanas, hispanas (latinas) y chicanas que vivían en New Mexico a mediados de 1940 y utilizar información fácilmente disponible sobre la alimentación y el estilo de vida de las poblaciones blancas. Estas entrevistas representan un paso importante para hacer estimaciones de las dosis de radiación generadas por la prueba Trinity.

  3. Pincharse sin infectarse: estrategias para prevenir la infección por el VIH y el VHC entre usuarios de drogas inyectables

    PubMed Central

    MATEU-GELABERT, P.; FRIEDMAN, S.; SANDOVAL, M.

    2011-01-01

    Resumen Objetivo Desde principios de los noventa, en la ciudad de Nueva York se han implementado con éxito programas para reducir la incidencia del virus de la inmunodeficiencia humana (VIH) y, en menor medida, del virus de la hepatitis C (VHC). A pesar de ello, aproximadamente el 70% de los usuario de drogas inyectables (UDI) están infectados por el VHC. Queremos investigar cómo el 30% restante se las ha arreglado para no infectarse. El Staying safe (nombre original del estudio) explora los comportamientos y mecanismos que ayudan a evitar la infección por el VHC y el VIH a largo plazo. Material y métodos Hemos utilizado el concepto de «desviación positiva» aplicado en otros campos de salud pública. Estudiamos las estrategias, prácticas y tácticas de prevención de aquellos UDI que, viviendo en contextos de alta prevalencia, se mantienen sin infectar por VIH y el VHC, a pesar de haberse inyectado heroína durante años. Los resultados preliminares presentados en este artículo incluyen el análisis de las entrevistas realizadas a 25 UDI (17 doble negativos, 3 doble positivos y 5 con infección por el VHC y sin infección por el VIH). Se usaron entrevistas semiestructuradas que exploraban con detalle la historia de vida de los sujetos, incluyendo su consumo de drogas, redes sociales, contacto con instituciones, relaciones sexuales y estrategias de protección y vigilancia. Resultados La intencionalidad es importante para no infectarse, especialmente durante períodos de involución (períodos donde hay un deterioro económico y/o social que llevan al que se inyecta a situaciones de mayor riesgo). Presentamos tres dimensiones independientes de intencionalidad que conllevan comportamientos que pueden ayudar a prevenir la infección: a) evitar «el mono» (síntomas de abstención) asegurando el acceso a la droga; b) «llevarlo bien» para no convertirse en un junkie y así evitar la «muerte social» y la falta de acceso a los recursos, y c) seguir sin infectarse por el VIH (sólo un doble negativo tuvo un amplio conocimiento sobre la hepatitis C). Estas intencionalidades no son mutuamente excluyentes. La presencia de varias refuerza la puesta en práctica a diario de comportamientos que pueden ayudar al que se inyecta a mantenerse libre de infecciones durante años. Algunas prácticas que hemos identificado se implementan en grupo y se comunican de UDI a UDI, de esta manera se extienden entre algunas redes sociales de UDI. Conclusiones Los UDI que permanecen sin infectarse planean e implementan estrategias de prevención en circunstancias donde otros UDI aplican prácticas de riesgo. El mantenimiento de la no infección no es, por lo tanto, un resultado del azar, sino más bien el resultado del esfuerzo (agencia) de los UDI. Investigar y extender estas estrategias y tácticas a través de programas de prevención podría contribuir a la prevención del VIH y el VHC. PMID:21915175

  4. [Bacteriological, pharmacokinetic and clinical studies of SY5555 dry syrup in the pediatric field].

    PubMed

    Toyonaga, Y; Ishihara, T; Tezuka, T; Nakamura, H

    1995-01-01

    Bacteriological, pharmacokinetic and clinical studies on SY5555 dry syrup (powder which is dissolved before use), a new penem antibiotic for oral use, were performed. The following results were obtained. 1. Antibacterial activities. MICs of SY5555, clavulanic acid/amoxicillin (CVA/AMPC), cefotiam (CTM), cefpodoxime (CPDX), cefaclor (CCL) and cefdinir (CFDN) were determined against clinically isolated Staphylococcus aureus, coagulase negative staphylococci, Streptococcus pneumoniae, Streptococcus pyogenes, Haemophilus influenzae, Moraxella catarrhalis, Escherichia coli and Enterobacter cloacae at a dose of 10(6) CFU/ml. MICs of SY5555 against S. aureus, CNS, S. pneumoniae, S. pyogenes, H. influenzae, M. catarrhalis, E. coli and E. cloacae were 0.2, 0.2, 0.2, < or = 0.025, 0.78, 0.2, 0.78 and 3.13 micrograms/ml, respectively, showing excellent antibacterial effects on these pathogens. Although the effects of SY 5555 against H. influenzae and E. coli were slightly inferior to those of CPDX and CFDN, the drug showed the most excellent antibacterial effect on other strains as compared with the control drugs. 2. Absorption and excretion In this study, plasma concentrations and urinary recovery rates were examined after administration of SY5555 at doses of 5 and 10 mg/kg (potency) after meals. With both 5 and 10 mg/kg doses, peak plasma concentrations were reached 1 hour after administration, at 0.25-2.61 micrograms/ml (mean 1.47 micrograms/ml) and 1.08-2.17 micrograms/ml (mean 1.74 micrograms/ml), respectively. The plasma levels rapidly decreased to 0.06-0.19 micrograms/ml (0.12 micrograms/ml) and 0.0503-0.0637 micrograms/ml) after 6 hours. The half-lives 1.12 hours in the 5 mg/kg group and 1.0 hour in the 10 mg/kg group. The urinary recovery rates were determined in the first 8 hours after administration in the 5 mg/kg and 6 hours in the 10 mg/kg group, and the values were as low as 1.05-12.3% and 1.6-4.33%, respectively. 3. Clinical results The clinical responses were examined in a total of 73 cases including 4 acute pneumonia, 13 acute bronchitis, 11 tonsillitis, 3 pharyngitis, 12 scarlet fever, 2 pertussis, 6 urinary tract infection, 6 otitis media, 7 lymphadenitis, 2 staphylococcal scalded skin syndrome, 2 phlegmon, 4 impetigo and 1 purulent parotitis. The treatment was effective or better in 66 of 70 cases with an efficacy rate of 94.3% (3 undeterminable cases were excluded). Bacteriological effects were examined during the clinical course for detected or suspected pathogens found before administration of SY5555. The effects were determined in 50 cases including 7 cases of polymicrobacterial infections, 57 strains in total. Eight strains, however, persisted, hence the overall eradication rate was 86.0%.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:7699846

  5. Flavobacterium johnsoniae PorV Is Required for Secretion of a Subset of Proteins Targeted to the Type IX Secretion System

    PubMed Central

    Kharade, Sampada S.

    2014-01-01

    Flavobacterium johnsoniae exhibits gliding motility and digests many polysaccharides, including chitin. A novel protein secretion system, the type IX secretion system (T9SS), is required for gliding and chitin utilization. The T9SS secretes the cell surface motility adhesins SprB and RemA and the chitinase ChiA. Proteins involved in secretion by the T9SS include GldK, GldL, GldM, GldN, SprA, SprE, and SprT. Porphyromonas gingivalis has orthologs for each of these that are required for secretion of gingipain protease virulence factors by its T9SS. P. gingivalis porU and porV have also been linked to T9SS-mediated secretion, and F. johnsoniae has orthologs of these. Mutations in F. johnsoniae porU and porV were constructed to determine if they function in secretion. Cells of a porV deletion mutant were deficient in chitin utilization and failed to secrete ChiA. They were also deficient in secretion of the motility adhesin RemA but retained the ability to secrete SprB. SprB is involved in gliding motility and is needed for formation of spreading colonies on agar, and the porV mutant exhibited gliding motility and formed spreading colonies. However, the porV mutant was partially deficient in attachment to glass, apparently because of the absence of RemA and other adhesins on the cell surface. The porV mutant also appeared to be deficient in secretion of numerous other proteins that have carboxy-terminal domains associated with targeting to the T9SS. PorU was not required for secretion of ChiA, RemA, or SprB, indicating that it does not play an essential role in the F. johnsoniae T9SS. PMID:25331433

  6. Spectrum of activity, mutation rates, synergistic interactions, and the effects of pH and serum proteins for fusidic acid (CEM-102).

    PubMed

    Biedenbach, Douglas J; Rhomberg, Paul R; Mendes, Rodrigo E; Jones, Ronald N

    2010-03-01

    Fusidic acid (CEM-102) is a steroidal antimicrobial agent with focused Gram-positive activity that acts by preventing bacterial protein synthesis via interacting with elongation factor G. A collection of 114 wild-type isolates (> 80 species) was used to define the contemporary limits of fusidic acid spectrum against Gram-positive and Gram-negative species. Reference broth microdilution and anaerobic agar dilution methods were performed. Modifications of standardized test methods included adding 10% human serum and adjusting the medium pH to 5, 6, and 8. Synergy was assessed by the checkerboard method and time-kill studies. Mutational rates to resistance were determined at 4 x, 8 x, and 16 x MIC. Against Gram-positive pathogens, fusidic acid MIC values ranged from 0.06 to 32 microg/mL with the greatest potency against Staphylococcus aureus, Corynebacterium spp., and Micrococcus luteus (MIC results, 0.25, < or = 0.12, and < or = 0.5 microg/mL, respectively). Enterococci and streptococci were less susceptible (MIC ranges, 2-8 and 16-32 microg/mL, respectively). Fusidic acid activity against Gram-negative species was more limited (all MIC values, > or = 2 microg/mL) except for Empedobacter brevis, Moraxella catarrhalis and Neisseria meningitidis. A 4-fold increase in fusidic acid MIC results was observed when 10% serum was added to the broth. Decreasing medium pH to 5.0 to 6.0 negated the protein binding effects. Among the 8 antimicrobial combinations tested, gentamicin and rifampin enhanced the activity when combined with fusidic acid (no antagonism). Fusidic acid in vitro activity was most improved when combined with rifampin. Single-step mutational rates ranged from 1.2 x 10(-6) for 4x MIC to 9.8 x 10(-8) for 16 x MIC. In conclusion, these in vitro results for fusidic acid tested against contemporary strains confirm a persisting antimicrobial spectrum, especially against staphylococci and some other Gram-positive species. PMID:20159376

  7. Ceftobiprole activity against over 60,000 clinical bacterial pathogens isolated in Europe, Turkey, and Israel from 2005 to 2010.

    PubMed

    Farrell, David J; Flamm, Robert K; Sader, Helio S; Jones, Ronald N

    2014-07-01

    Ceftobiprole medocaril is a newly approved drug in Europe for the treatment of hospital-acquired pneumonia (HAP) (excluding patients with ventilator-associated pneumonia but including ventilated HAP patients) and community-acquired pneumonia in adults. The aim of this study was to evaluate the in vitro antimicrobial activity of ceftobiprole against prevalent Gram-positive and -negative pathogens isolated in Europe, Turkey, and Israel during 2005 through 2010. A total of 60,084 consecutive, nonduplicate isolates from a wide variety of infections were collected from 33 medical centers. Species identification was confirmed, and all isolates were susceptibility tested using reference broth microdilution methods. Ceftobiprole had high activity against methicillin-susceptible Staphylococcus aureus (MSSA) (100.0% susceptible), methicillin-susceptible coagulase-negative staphylococci (CoNS), beta-hemolytic streptococci, and Streptococcus pneumoniae (99.3% susceptible), with MIC90 values of 0.25, 0.12, ? 0.06, and 0.5 ?g/ml, respectively. Ceftobiprole was active against methicillin-resistant S. aureus (MRSA) (98.3% susceptible) and methicillin-resistant CoNS, having a MIC90 of 2 ?g/ml. Ceftobiprole was active against Enterococcus faecalis (MIC50/90, 0.5/4 ?g/ml) but not against most Enterococcus faecium isolates. Ceftobiprole was very potent against the majority of Enterobacteriaceae (87.3% susceptible), with >80% inhibited at ? 0.12 ?g/ml. The potency of ceftobiprole against Pseudomonas aeruginosa (MIC50/90, 2/>8 ?g/ml; 64.6% at MIC values of ? 4 ?g/ml) was similar to that of ceftazidime (MIC50/90, 2/>16 ?g/ml; 75.4% susceptible), but limited activity was observed against Acinetobacter spp. and Stenotrophomonas maltophilia. High activity was also observed against all Haemophilus influenzae (MIC90, ? 0.06 ?g/ml) and Moraxella catarrhalis (MIC50/90, ? 0.06/0.25 ?g/ml) isolates. Ceftobiprole demonstrated a wide spectrum of antimicrobial activity against this very large longitudinal sample of contemporary pathogens. PMID:24777091

  8. Observations from a multicentre study on the use of the sputum specimen in patients hospitalized with community-acquired pneumonia

    PubMed Central

    Taylor, EL; Marrie, TJ; Fine, MJ; Obroskyl, DS; Kapoor, WN; Coley, C; Singer, DE

    1999-01-01

    OBJECTIVE: To describe the use of sputum Gram stain and culture in patients with community-acquired pneumonia (CAP) and to determine the factors that are associated with obtaining sputum for culture. TYPE OF STUDY: Prospective observational cohort study of patients hospitalized for treatment of CAP at four medical institutions in three geographic locations. MAIN MEASUREMENTS: Results of Gram stain and culture of sputum; comparison of patients who had sputum processed for culture within 24 h of admission with those who did not have such a specimen processed during the first week of hospitalization; and the results of investigator assignment of etiology of pneumonia according to predefined criteria. RESULTS: Four hundred and seventy-eight of 1339 (36%) patients had a sputum specimen processed for culture within 24 h of admission. Patients who had a sputum specimen processed within 24 h of admission were more likely to be hospitalized at the Boston site (odds ratio [OR] 20.6) or Pittsburgh sites (3.4) and to have current sputum production, chronic obstructive lung disease and moderate or large amount of sputum. Female sex (0.4), neutropenia (0.05), and do not resuscitate status (0.36) were important predictors of failure to have a sputum processed for culture. The rate of Streptococcus pneumoniae isolation was highest in Boston, 53 of 269 (19.3 %) patients (P<0.001) compared with the other sites; Moraxella catarrhalis was isolated only at the Boston site. Sputum culture results served as the basis for the assignment of an etiological diagnosis of the pneumonia by investigators in 67% of 397 patients. CONCLUSIONS: Sputum is not processed for culture in the majority of patients with CAP. The factors that determine whether sputum is processed for culture within 24 h of admission are site of care and a variety of patient factors. Common respiratory pathogens when present in sputum culture tend to be used to assign an etiological diagnosis. A positive sputum culture result appears not to result in a more favourable outcome. PMID:22346371

  9. [In vitro activities of levofloxacin and other antibiotics against fresh clinical isolates].

    PubMed

    Matsuzaki, K; Koyama, H; Chiba, A; Omika, K; Harada, S; Sato, Y; Hasegawa, M; Kobayashi, I; Kaneko, A; Sasaki, J

    1999-09-01

    In this study, the in vitro activity of levofloxacin (LVFX) against 1,020 fresh bacterial clinical isolates was compared with the activities of a range of ofloxacin, ciprofloxacin (CPFX), ampicillin (ABPC), cefaclor, cefpodoxime, methicillin and benzylpenicillin. The clinical isolates except Vibrio cholerae were collected in Japan during 1998 from patients with infectious diseases. MICs were determined using the agar dilution method according to the recommendations by the Japan Society of Chemotherapy. Some isolates of methicillin resistant Staphylococcus aureus (MRSA) and coagulase negative Staphylococcus were resistant to fluoroquinolones, but the MIC50 of LVFX against MRSA was 6.25 micrograms/ml. LVFX was the most active against MRSA among the antibiotics tested. Most of Staphylococcus epidermidis strains were susceptible to the fluoroquinolones. LVFX showed greater activity against all streptococci strains compared with fluoroquinolones tested. In particular, all Streptococcus pneumoniae strains including PRSP were susceptible to LVFX at < or = 1.56 micrograms/ml. Among Enterococcus, ABPC showed superior activity against Enterococcus faecalis but many isolates of Enterococcus species were resistant to ABPC. LVFX was more active against to these Enterococcus species compared with other fluoroquinolones. On the other hand, LVFX and CPFX showed similar activity against isolates of Enterobacteriaceae. CPFX had an MIC50/90 of 0.20, 0.39 microgram/ml and LVFX showed an MIC50/90 of 0.78, 1.56 micrograms/ml against Pseudomonas aeruginosa. LVFX (MIC50/90 0.10, 0.20 microgram/ml) was more active against Acinetobacter species than CPFX (MIC50/90 0.10, 0.39 microgram/ml). Haemophilus influenzae, Branhamella (Moraxella) catarrhalis and V. cholerae were inhibited by low concentration of the fluoroquinolones tested. The MIC90 of LVFX and CPFX were < or = 0.10 microgram/ml against above three species. Some isolates of Neisseria gonorrhoeae and Campylobacter species were moderately resistant to the fluoroquinolones tested but the MIC50 of LVFX and CPFX were < or = 0.39 microgram/ml. Among anaerobes, Propionibacterium acnes was more susceptible than Peptostreptococcus species, and the MIC90 of beta-lactams and fluoroquinolones tested were < or = 0.78 microgram/ml. In conclusion, this study, performed on large number of strains, confirmed an excellent and wide spectrum antibacterial activity of LVFX compared with the fluoroquinolones and beta-lactams tested. And our results suggest that LVFX may be useful in the treatment of various bacterial infections. PMID:10746192

  10. Antimicrobial Activity of the Pleuromutilin Antibiotic BC-3781 against Bacterial Pathogens Isolated in the SENTRY Antimicrobial Surveillance Program in 2010

    PubMed Central

    Sader, Helio S.; Ivezic-Schoenfeld, Zrinka; Jones, Ronald N.

    2013-01-01

    BC-3781 is a novel semisynthetic pleuromutilin antibiotic inhibiting bacterial protein synthesis. BC-3781 has completed a phase 2 clinical trial in acute bacterial skin and skin structure infections (ABSSSI). Its antibacterial spectrum additionally covers the predominant pathogens causing community-acquired bacterial pneumonia (CABP). In this study, the antibacterial activity of BC-3781 was evaluated against a contemporary collection of 10,035 bacterial isolates predominately causing ABSSSI and CABP, among other infections, collected within the SENTRY Antimicrobial Surveillance Program worldwide in 2010. BC-3781 exhibited potent activity against organisms commonly isolated from ABSSSI such as Staphylococcus aureus (MIC50/90, 0.12/0.12 ?g/ml; 99.8% inhibited at ?0.5 ?g/ml), beta-hemolytic streptococci (MIC50/90, 0.03/0.03 ?g/ml; 99.3% inhibited at ?0.5 ?g/ml), and coagulase-negative staphylococci (CoNS; MIC50/90, 0.06/0.12 ?g/ml; 97.8% inhibited at ?1 ?g/ml). BC-3781 displayed similar MIC distributions among methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) S. aureus strains. BC-3781 was also active against Enterococcus faecium, with 76.3% of vancomycin-susceptible and 97.0% of vancomycin-resistant isolates being inhibited at BC-3781 concentrations of ?1 ?g/ml. Beta-hemolytic and viridans group streptococci were highly susceptible to BC-3781, with 99.3% and 96.7% of isolates inhibited at ?0.5 ?g/ml, respectively. Further, activity of BC-3781 against Streptococcus pneumoniae (MIC50/90, 0.12/0.25 ?g/ml), Haemophilus influenzae (MIC50/90, 1/2 ?g/ml), and Moraxella catarrhalis (MIC50/90, 0.12/0.25 ?g/ml) was not negatively influenced by ?-lactamase production or resistance to other antimicrobial classes tested. In all, BC-3781 displayed a very potent antibacterial profile including the most prevalent bacterial pathogens causing ABSSSI and CABP, thus warranting further clinical development of this antibiotic in these and possibly other indications. PMID:23836172

  11. Ceftobiprole Activity against over 60,000 Clinical Bacterial Pathogens Isolated in Europe, Turkey, and Israel from 2005 to 2010

    PubMed Central

    Flamm, Robert K.; Sader, Helio S.; Jones, Ronald N.

    2014-01-01

    Ceftobiprole medocaril is a newly approved drug in Europe for the treatment of hospital-acquired pneumonia (HAP) (excluding patients with ventilator-associated pneumonia but including ventilated HAP patients) and community-acquired pneumonia in adults. The aim of this study was to evaluate the in vitro antimicrobial activity of ceftobiprole against prevalent Gram-positive and -negative pathogens isolated in Europe, Turkey, and Israel during 2005 through 2010. A total of 60,084 consecutive, nonduplicate isolates from a wide variety of infections were collected from 33 medical centers. Species identification was confirmed, and all isolates were susceptibility tested using reference broth microdilution methods. Ceftobiprole had high activity against methicillin-susceptible Staphylococcus aureus (MSSA) (100.0% susceptible), methicillin-susceptible coagulase-negative staphylococci (CoNS), beta-hemolytic streptococci, and Streptococcus pneumoniae (99.3% susceptible), with MIC90 values of 0.25, 0.12, ?0.06, and 0.5 ?g/ml, respectively. Ceftobiprole was active against methicillin-resistant S. aureus (MRSA) (98.3% susceptible) and methicillin-resistant CoNS, having a MIC90 of 2 ?g/ml. Ceftobiprole was active against Enterococcus faecalis (MIC50/90, 0.5/4 ?g/ml) but not against most Enterococcus faecium isolates. Ceftobiprole was very potent against the majority of Enterobacteriaceae (87.3% susceptible), with >80% inhibited at ?0.12 ?g/ml. The potency of ceftobiprole against Pseudomonas aeruginosa (MIC50/90, 2/>8 ?g/ml; 64.6% at MIC values of ?4 ?g/ml) was similar to that of ceftazidime (MIC50/90, 2/>16 ?g/ml; 75.4% susceptible), but limited activity was observed against Acinetobacter spp. and Stenotrophomonas maltophilia. High activity was also observed against all Haemophilus influenzae (MIC90, ?0.06 ?g/ml) and Moraxella catarrhalis (MIC50/90, ?0.06/0.25 ?g/ml) isolates. Ceftobiprole demonstrated a wide spectrum of antimicrobial activity against this very large longitudinal sample of contemporary pathogens. PMID:24777091

  12. Se entiende por lenguaje sexista, no incluyente o no integrador el uso exclusivo de uno de los dos gneros (habitualmente el masculino) para

    E-print Network

    especificación de sexo o construcciones impersonales : o Los investigadores el personal investigador o Como brillantes Algunas mentes brillantes Sustituir las construcciones copulativas que exijan un adjetivo en masculino por otras construcciones: ej. tener o sentir + sustantivo o construcciones reflexivas o

  13. Revista Iberoamericana de Automtica e Informtica industrial 12 (2015) 166176 2013 CEA. Publicado por Elsevier Espaa, S.L.U. Todos los derechos reservados

    E-print Network

    Batlle, Carles

    2015-01-01

    . Publicado por Elsevier España, S.L.U. Todos los derechos reservados http://dx.doi.org/10.1016/j.riai.2015.L. Todos los derechos reservados. Palabras Clave: fuzzy control, autonomous vehicles, linear control

  14. Relación masa-radio para estrellas enanas blancas y la interpretación de recientes mediciones hechas por Hipparcos

    NASA Astrophysics Data System (ADS)

    Panei, J. A.; Althaus, L. G.; Benvenuto, O. G.

    Recientes mediciones de la masa y el radio hechas por Hipparcos de las estrellas enanas blancas 40 Eri B y Procyon B (Shipman, H. & Provencal, J. - ApJ. 1998, 494, 759), sugieren un núcleo compuesto de hierro para dichas estrellas, en lugar de carbono y oxígeno como predice la teoría standard de evolución estelar. Para interpretar estas observaciones, presentamos aquí, relaciones masa-radio para configuraciones degeneradas a temperatura finita para distintas composiciones químicas centrales. Para tal fin hemos calculado secuencias evolutivas de enanas blancas utilizando el código de evolución estelar, desarrollado en el Observatorio de La Plata. Dicho código resuelve las ecuaciones de estructura y evolución estelar mediante la técnica de relajación de Henyey, y esta basado en una descripción física muy detallada y actualizada.

  15. Immunogenicity and reactogenicity in UK infants of a novel meningococcal vesicle vaccine containing multiple class 1 (PorA) outer membrane proteins.

    PubMed

    Cartwright, K; Morris, R; Rümke, H; Fox, A; Borrow, R; Begg, N; Richmond, P; Poolman, J

    1999-06-01

    The development of effective vaccines against serogroup B meningococci is of great public health importance. We assessed a novel genetically engineered vaccine containing six meningococcal class 1 (PorA) outer membrane proteins representing 80% of prevalent strains in the UK. 103 infants were given the meningococcal vaccine at ages 2, 3 and 4 months with routine infant immunisations, with a fourth dose at 12-18 months. The vaccine was well tolerated. Three doses evoked good immune responses to two of six meningococcal strains expressing PorA proteins contained in the vaccine. Following a fourth dose, larger bactericidal responses to all six strains were observed, suggesting that the initial course had primed memory lymphocytes and revaccination stimulated a booster response. This hexavalent PorA meningococcal vaccine was safe and evoked encouraging immune responses in infants. Vaccines of this type warrant further development and evaluation. PMID:10418910

  16. "Estudio tribologico de aceros para moldes. Aplicacion al moldeo por inyeccion de polibutilentereftalato reforzado con fibra de vidrio"

    NASA Astrophysics Data System (ADS)

    Martinez Mateo, Isidoro Jose

    Mould materials for injection moulding of polymers and polymer-matrix composites represent a relevant industrial economic sector due to the large quantity of pieces and components processed. The material selection for mould manufacturing, its composition and heat treatment, the hardening procedures and machining and finishing processes determine the service performance and life of the mould. In the first part of the present study, the relationship between the hardness and microstructure and the wear resistance of mould steels from large blocks has been studied by pin-on-disc tests, studying the main wear mechanisms. In order to determine the surface damage on mould steels under real injection conditions, different commercial steels have been studied by measuring the variation of surface roughness with the number of injected pieces with different reinforcement percentages and different mould geometries, by using optical profilometry and scanning electron microscopy techniques. It was important to determine the variation of surface roughness of the moulded pieces with the number of injection operations. The materials used were polybutyleneterephthalate pure and reinforced with either 20% or 50% glass fibre. For the different mould designs, the evolution of the glass fibre orientation with injection flow has been determined by image analysis and related to roughness changes and surface damage, both of the composite parts and of the mould steel surface. Finally, the abrasion resistance of the composite parts has been studied by scratch tests as a function of the number of injected parts and of the scratch direction with respect to injection flow and glass fibre orientation. Los materiales para moldes de inyeccion de polimeros y materiales compuestos representan un sector economicamente muy relevante debido al gran aumento del numero de componentes fabricados a partir de materiales polimericos obtenidos mediante moldeo por inyeccion. La seleccion del material para la fabricacion del molde, tienen una gran influencia sobre su comportamiento en servicio a lo largo de la vida util del molde. En la primera parte del presente estudio, a partir de ensayos punzon sobre disco, se ha determinado la relacion entre la resistencia al desgaste y la dureza de aceros para moldes obtenidos a partir de bloques de gran espesor, estudiando los principales mecanismos de desgaste que tienen lugar. A continuacion, con el fin de determinar el dano superficial que sufren los aceros para moldes en condiciones reales de inyeccion, se han estudiado distintos tipos de aceros utilizados comercialmente en moldes de inyeccion de polimeros y materiales compuestos, seleccionando las condiciones de operacion para determinar la variacion de la rugosidad superficial del acero en funcion del material inyectado, del numero de operaciones sucesivas de inyeccion y de la orientacion del flujo de inyeccion, mediante tecnicas de perfilometria optica y microscopia electronica de barrido. Ademas del dano superficial sufrido por el acero con el numero de piezas inyectadas, tambien se ha determinado la evolucion de la rugosidad superficial de los materiales inyectados, polibutilentereftalato (PBT) puro y materiales compuestos derivados de PBT por adicion de un 20 o un 50% en peso de fibra de vidrio. En el caso de las piezas inyectadas, se ha caracterizado su microestructura en funcion del flujo de inyeccion y de la densidad de fibra, se han determinado sus propiedades termicas y dinamico-mecanicas, asi como la variacion de la rugosidad superficial de las piezas inyectadas con el numero de operaciones de inyeccion y con la geometria de las distintas secciones de las piezas. Finalmente, se ha evaluado la resistencia a la abrasion de PBT reforzado con un 50% de fibra, en funcion del numero de piezas inyectadas y de la direccion de rayado con respecto a la orientacion del flujo de inyeccion.

  17. Estudio teórico de la desorción de Na y K de SiO2 estimulada por la acción de fotones o electrones

    NASA Astrophysics Data System (ADS)

    Domínguez Ariza, D.; López, N.; Illas, F.; Pacchioni, G.; Madey, T. E.

    Se ha estudiado el mecanismo de generación de sodio y potasio atómico a partir de muestras de SiO2 utilizando cálculos basados tanto en la teoría del funcional de la densidad como en métodos post-Hartree Fock, así como en el método de cluster para modelar el sólido. Como consecuencia del estudio se han propuesto distintos caminos posibles para la desorción, estimulada por la acción de fotones o electrones, de sodio y potasio desde el óxido de silicio, proporcionando por lo tanto una explicación a la atmósfera tenue de sodio y potasio de La Luna.

  18. Fuentes de variabilidad en el diagnóstico de gastritis atrófica multifocal asociada con la infección por Helicobacter pylori1

    PubMed Central

    Bravo, Luis Eduardo; Bravo, Juan Carlos; Realpe, José Luis; Zarama, Guillermo; Piazuelo, MarÍa Blanca; Correa, Pelayo

    2014-01-01

    RESUMEN Introducción El mapeo de las diferentes regiones del estómago y el número de fragmentos de mucosa gástrica disponibles para evaluación histopatológica son fuentes importantes de variación en el momento de clasificar y hacer la gradación de la gastritis crónica. Objetivos Estimar la sensibilidad del número de fragmentos de mucosa gástrica necesarios para establecer los diagnósticos de gastritis atrófica con metaplasia intestinal (MI), displasia y estado de infección por Helicobacter pylori. Además evaluar la variabilidad intra-observador en la clasificación de estas lesiones precursoras del cáncer gástrico. Materiales y métodos En una cohorte de 6 años de seguimiento se evaluaron 1,958 procedimientos de endoscopia realizados por dos gastroenterólogos. En cada procedimiento y de cada participante se obtuvieron 5 biopsias de mucosa gástrica que representaban antro, incisura angularis y cuerpo. Un único patólogo hizo la interpretación histológica de las 5 biopsias y proporcionó un diagnóstico definitivo global que se utilizó como patrón de referencia. Cada fragmento de mucosa gástrica examinado condujo a un diagnóstico individual para cada biopsia que se comparó con el patrón de referencia. La variabilidad intra-observador se evaluó en 127 personas que corresponden a una muestra aleatoria de 20% del total de endoscopias hechas a los 72 meses de seguimiento. Resultados La sensibilidad del diagnóstico de MI y displasia gástrica aumentó de manera significativa con el número de fragmentos de mucosa gástrica evaluados El sitio anatómico de mayor sensibilidad para el diagnóstico de MI y displasia fue la incisura angularis. Para descubrir H. pylori se logró alta sensibilidad con el estudio de un solo fragmento de mucosa gástrica (95.9%) y fue independiente del sitio de obtención de la biopsia. El acuerdo intra-observador para el diagnóstico de gastritis crónica fue 86.1% con valor kappa de 0.79 IC 95% (0.76-0.85). Las biopsias fijadas en alcohol fueron inadecuadas para demostrar H. pylori e interpretar los cambios de displasia. Conclusión El número de fragmentos de mucosa gástrica evaluados, el método de fijación y la región del estómago donde se obtienen las biopsias son factores muy importantes para lograr una correcta clasificación de la gastritis crónica atrófica multifocal. PMID:25267864

  19. Spectroscopic and kinetic characterization of the light-dependent enzyme protochlorophyllide oxidoreductase (POR) using monovinyl and divinyl substrates.

    PubMed

    Heyes, Derren J; Kruk, Jerzy; Hunter, C Neil

    2006-02-15

    The enzyme POR [Pchlide (protochlorophyllide) oxidoreductase] catalyses the reduction of Pchlide to chlorophyllide, which is a key step in the chlorophyll biosynthesis pathway. This light-dependent reaction has previously been studied in great detail but recent reports suggest that a mixture of MV (monovinyl) and DV (divinyl) Pchlides may have influenced some of these properties of the reaction. Low-temperature absorbance and fluorescence spectroscopy have revealed several spectral differences between MV and DV Pchlides, which were purified from a Rhodobacter capsulatus strain that was shown to contain a mixture of the two pigments. A thorough steady-state kinetic characterization using both Pchlide forms demonstrates that neither pigment appears to affect the kinetic properties of the enzyme. The reaction has also been monitored following illumination at low temperatures and was shown to consist of an initial photochemical step followed by four 'dark' steps for both pigments. However, minor differences were observed in the spectral properties of some of the intermediates, although the temperature dependency of each step was nearly identical for the two pigments. This work provides the first detailed kinetic and spectroscopic study of this unique enzyme using biologically important MV and DV substrate analogues. It also has significant implications for the DV reductase enzyme, which is responsible for converting DV pigments into their MV counterparts, and its position in the sequence of reactions that comprise the chlorophyll biosynthesis pathway. PMID:16274361

  20. Nuevo sitio web en español del Instituto Nacional del Cáncer, (NCI, por sus siglas en inglés) Cancer.gov en español - Silvia Inéz Salazar - transcript

    Cancer.gov

    Transmisiones de radio para promover Cancer.gov en espa%XF1ol | Nuevo sitio web en espa%XF1ol del Instituto Nacional del C%XE1ncer, (NCI, por sus siglas en ingl%XE9s) Cancer.gov en espa%XF1ol | Transcripci%XF3n Transmisiones de radio para promover

  1. Qu es el NICHD? El Instituto Nacional de Salud Infantil y Desarrollo Humano Eunice Kennedy Shriver (NICHD, por sus siglas en

    E-print Network

    Rau, Don C.

    Descubra el NICHD ¿Qué es el NICHD? El Instituto Nacional de Salud Infantil y Desarrollo Humano investigaciones sobre temas de salud. Creado en 1962, el Instituto es parte de los Institutos Nacionales de la Salud (NIH, por sus siglas en inglés) del Departamento de Salud y Servicios Humanos de los Estados

  2. El proceso hacia la integracion de la equidad por genero al curriculo.(The Process of the Integration of Gender Equity in the Curriculum.)

    ERIC Educational Resources Information Center

    Rivera-Bermudez, Carmen D.

    "El Proyecto Colaborativo de Equidad por Genero en la Educacion," or the Collaborative Project for Gender Equity in Education, was undertaken in Puerto Rico between 1990 and 1992 to study how to facilitate the integration of gender equity themes in the curriculum through the direct action of participating teachers. A study examined the attitudes…

  3. Instituciones al Servicio de los Hispanos están a la vanguardia de la investigación de las desigualdades en salud por cáncer en la comunidad latina

    Cancer.gov

    Varias Instituciones al Servicio de los Hispanos forman parte de un grupo de escuelas profesionales y universidades que se han asociado con el Instituto Nacional del Cáncer (NCI) y cierto número de centros oncológicos regionales para abordar el tema de las desigualdades en salud por cáncer que afectan a la comunidad latina.

  4. Eficacia de la detección sistemática de la gripe en las fronteras en los viajeros que llegan por vía aérea*

    PubMed Central

    Priest, Patricia C.; Jennings, Lance C.; Duncan, Alasdair R.; Brunton, Cheryl R.; Baker, Michael G.

    2015-01-01

    Objetivos. Se midieron los síntomas y la prevalencia de la gripe (también llamada influenza), así como la eficacia del mecanismo de detección sistemática basado en los síntomas y la temperatura para diagnosticar la gripe en viajeros internacionales que llegaban por vía aérea. Métodos. El presente estudio transversal recopiló datos de viajeros que llegaron al aeropuerto internacional de Christchurch (Nueva Zelandia) en el invierno del 2008 mediante un cuestionario de salud, medición de la temperatura y toma de muestras de las vías respiratorias. Resultados. De los viajeros, 15?976 (68%) entregaron los formularios completos. De ellos, 17% notificaron al menos un síntoma de gripe; los síntomas más comunes fueron rinorrea o congestión nasal (10%) y tos (8%). Se tomaron muestras de las vías respiratorias de 3?769 viajeros. La prevalencia estimada de la gripe fue de 1,1% (4% en las personas sintomáticas, 0,2% en las asintomáticas). La sensibilidad de los criterios de detección varió de 84% para “cualquier síntoma” a 3% para la fiebre de 37,8 °C o mayor. El valor predictivo positivo fue bajo para todos los criterios. Conclusiones. El método de detección sistemática en las fronteras mediante la autonotificación de síntomas y la toma de la temperatura presenta limitaciones para impedir que una gripe pandémica entre en un país. Basarse en criterios como “cualquier síntoma” o la tos haría que se investigara a varias personas no infectadas, mientras que algunas personas infectadas pasarían inadvertidas. Si se usaran criterios más específicos como la fiebre, la mayoría de las personas infectadas entrarían en el país a pesar del mecanismo de detección.

  5. Por ms de 30 aos, el programa de CU Wizards ha presentado shows mensuales GRATIS que entretienen e informan a los nios sobre las maravillas de la ciencia. Estos shows interactivos se

    E-print Network

    Lineberger, W. Carl

    Por más de 30 años, el programa de CU Wizards ha presentado shows mensuales GRATIS que entretienen Outreach Program -Magos Locales Entusiastas que donan! Programa CU Wizards 2014-2015: 9/27/2014 Prof. Lew información por favor visite: www.colorado.edu/physics/Web/wizards/cuwizards.html #12;

  6. La inserción en el mercado laboral de los inmigrantes latinos en España y en los Estados Unidos: Diferencias por país de origen y estatus legal

    PubMed Central

    Connor, Phillip; Massey, Douglas

    2013-01-01

    Resumen Este artículo compara los resultados económicos entre los inmigrantes latinoamericanos en España y Estados Unidos. Detectamos un efecto de selección por el que la mayoría de los inmigrantes latinoamericanos en España proceden de Sudamérica de un entorno de clases medias, mientras la mayoría de los inmigrantes que van a los Estados Unidos son centroamericanos de clase baja. Este efecto de selección explica las diferencias transnacionales en la probabilidad de empleo, logro ocupacional y salarios obtenidos. A pesar de las diferencias en los orígenes y las características de los latinoamericanos en ambos países, los factores demográficos, humanos y de capital social parecen operar de forma similar en ambos países; y cuando los modelos se estiman separadamente por estatus legal, descubrimos que los efectos se acentúan más entre los inmigrantes irregulares cuando se los compara con los regulares, especialmente en Estados Unidos. PMID:24532857

  7. Study of the interaction mechanisms between absorbed NO{sub 2} and por-Si/SnO{sub x} nanocomposite layers

    SciTech Connect

    Bolotov, V. V.; Kan, V. E. Makushenko, R. K.; Biryukov, M. Yu.; Ivlev, K. E.; Roslikov, V. E.

    2013-10-15

    The interaction mechanisms between NO{sub 2} molecules and the surface of por-Si/SnO{sub x} nanocomposites obtained by magnetron deposition and chemical vapor deposition (CVD) are studied by infrared absorption spectroscopy and electron paramagnetic resonance methods. The observed increase in the free carrier concentration in the por-Si/SnO{sub x} nanocomposite layers is explained by a change in the charge state of P{sub b} centers due to the formation of neutral 'surface defect-adsorbed NO{sub 2} molecule' complexes with free carrier generation in the crystallite bulk. In the nanocomposite layers grown by the CVD method, the increase in the free hole concentration during NO{sub 2} adsorption is much less pronounced in comparison with the composite grown by magnetron deposition, which is caused by the competing interaction channel of NO{sub 2} molecules with electrically neutral P{sub b} centers.

  8. Immunogenicity studies with a genetically engineered hexavalent PorA and a wild-type meningococcal group B outer membrane vesicle vaccine in infant cynomolgus monkeys.

    PubMed

    Rouppe van der Voort, E; Schuller, M; Holst, J; de Vries, P; van der Ley, P; van den Dobbelsteen, G; Poolman, J

    2000-01-31

    The immunogenicity of two meningococcal outer membrane vesicle (OMV) vaccines, namely the Norwegian wild-type OMV vaccine and the Dutch hexavalent PorA OMV vaccine, were examined in infant cynomolgus monkeys. For the first time, a wild-type- and a recombinant OMV vaccine were compared. Furthermore, the induction of memory and the persistence of circulating antibodies were measured. The Norwegian vaccine contained all four classes of major outer membrane proteins (OMP) and wild-type L3/L8 lipopolysaccharide (LPS). The Dutch vaccine consisted for 90% of class 1 OMPs, had low expression of class 4 and 5 OMP, and GalE LPS. Three infant monkeys were immunised with a human dose at the age of 1.5, 2.5 and 4.5 months. Two monkeys of each group received a fourth dose at the age of 11 months. In ELISA, both OMV vaccines were immunogenic and induced booster responses, particularly after the fourth immunisation. The Norwegian vaccine mostly induced sero-subtype P1.7,16 specific serum bactericidal antibodies (SBA), although some other SBA were induced as well. The antibody responses against P1.7,16, induced by the Norwegian vaccine, were generally higher than for the Dutch vaccine. However, the Dutch vaccine induced PorA specific SBA against all six sero-subtypes included in the vaccine showing differences in the magnitude of SBA responses to the various PorAs. PMID:10618530

  9. Variation in P450 oxidoreductase (POR) A503V and flavin containing monooxygenase (FMO)-3 E158K is associated with minor alterations in nicotine metabolism but does not alter cigarette consumption

    PubMed Central

    Chenoweth, Meghan J.; Zhu, Andy Z. X.; Cox, Lisa Sanderson; Ahluwalia, Jasjit S.; Benowitz, Neal L.; Tyndale, Rachel F.

    2014-01-01

    Nicotine metabolism rates differ widely, even after controlling for genetic variation in the major nicotine metabolizing enzyme, CYP2A6. Genetic variants in an additional nicotine metabolizing enzyme, flavin containing monooxygenase (FMO)-3, and an obligate microsomal CYP-supportive enzyme, cytochrome P450 oxidoreductase (POR), were investigated. We examined the impact of FMO3 E158K and POR A503V, before and after stratifying by CYP2A6 metabolism group. In 130 non-smokers of African descent who received 4 mg oral nicotine, FMO3 158K trended towards slower nicotine metabolism in reduced CYP2A6 metabolizers (P=0.07) only, whereas POR 503V was associated with faster CYP2A6 activity (nicotine metabolite ratio) in normal (P=0.03), but not reduced, CYP2A6 metabolizers. Neither FMO3 158K nor POR 503V significantly altered the nicotine metabolic ratio (N=659), cigarette consumption (N=667), or urine total nicotine equivalents (N=418) in smokers of African descent. Thus, FMO3 E158K and POR A503V are minor sources of nicotine metabolism variation, insufficient to appreciably alter smoking. PMID:24448396

  10. [Factors effecting the duration of hospitalization and mortality in patients with community-acquired pneumonia].

    PubMed

    Pi?kin, Nihal; Aydemir, Hande; Oztoprak, Nefise; Akduman, Deniz; Celebi, Güven; Seremet Keskin, Ay?egül

    2009-10-01

    Community-acquired pneumonia (CAP) is a common infectious disease with high morbidity and mortality. In this study, demographic features, underlying conditions, causative pathogens and factors affecting length of hospital stay and mortality were retrospectively investigated in patients who were diagnosed as CAP and followed-up in our unit between January 2005-December 2007. Among 97 patients 65 (67%) were male, 32 (33%) were female and the mean age was 62.7 (age range: 18-94) years. Patients were grouped according to criteria of Turkish Thoracic Society into four groups; 22 were group 2 (patients with risk factors, without aggrevating factors), 59 were group 3 (patients with aggrevating factors), and 16 were group 4 (patients who have necessity for intensive care) CAP. The patients have also been grouped according to criteria of American Thoracic Society (CURB-65 score = Confusion, Urea > 7 mmol/L, Respiratory rate > or = 30/min, low Blood pressure and being > or = 65 years old), as group I (n = 65), group II (n = 20), and group III (n = 12). During follow-up 11 (11.3%) patients required mechanical ventilation support and 6 (6.2%) patients have died. Causative pathogens were isolated from 14 (23.3%) out of 27 well-qualified sputum samples obtained from 60 patients who could produce sputum (8 Streptococcus pneumoniae, 2 methicillin-sensitive Staphylococcus aureus, 2 Klebsiella pneumoniae, 1 Haemophilus influenzae, 1 Moraxella catarrhalis). Thirty-seven of cases were treated with levofloxacin, 10 with moxifloxacin, 24 with ceftriaxone +/- clarithromycin, 16 with sulbactam-ampicillin +/- ciprofloxacin, 10 with beta-lactam/beta-lactamase inhibitor combinations, and fever declined within 2.5 days in 83 (85.6%) of them. The mean duration of hospital stay was estimated as 11.1 days. In the evaluation of the factors that affect the length of hospital stay, being > or = 65 years old, gender, underlying conditions, central venous catheterisation, presence of nasogastric tube, positive culture result, previous antibiotic treatment, fever continuing for > 3 days despite antibiotic therapy and scoring groups were not determined as risk factors (p > 0.05 for all of these parameters). However, mechanical ventilation was found as a significant risk factor (p < 0.05). In the evaluation of the factors that affect mortality, mechanical ventilation (p < 0.001), staying in intensive care unit (p < 0.001), being group 4 CAP (p < 0.001) and fever continuing for > 3 days despite antibiotic therapy (p = 0.05) were found to be significant risk factors. In conclusion, length of hospital stay, mortality and treatment costs in CAP patients could be reduced by defining the risk factors and starting empirical antibiotic therapy according to the national and international guidelines. PMID:20084912

  11. Neuropatía periférica inducida por quimioterapia

    Cancer.gov

    Artículo sobre un efecto secundario de la quimioterapia que causa dolor y malestar en las manos y los pies. También incluye información sobre los esfuerzos para mejorar las opciones de detección, tratamiento y prevención.

  12. IgE Reactivity of Blue Swimmer Crab (Portunus pelagicus) Tropomyosin, Por p 1, and Other Allergens; Cross-Reactivity with Black Tiger Prawn and Effects of Heating

    PubMed Central

    Varese, Nirupama; Zubrinich, Celia; Lopata, Andreas L.; O'Hehir, Robyn E.; Rolland, Jennifer M.

    2013-01-01

    Shellfish allergy is a major cause of food-induced anaphylaxis, but the allergens are not well characterized. This study examined the effects of heating on blue swimmer crab (Portunus pelagicus) allergens in comparison with those of black tiger prawn (Penaeus monodon) by testing reactivity with shellfish-allergic subjects' serum IgE. Cooked extracts of both species showed markedly increased IgE reactivity by ELISA and immunoblotting, and clinical relevance of IgE reactivity was confirmed by basophil activation tests. Inhibition IgE ELISA and immunoblotting demonstrated cross-reactivity between the crab and prawn extracts, predominantly due to tropomyosin, but crab-specific IgE-reactivity was also observed. The major blue swimmer crab allergen tropomyosin, Por p 1, was cloned and sequenced, showing strong homology with tropomyosin of other crustacean species but also sequence variation within known and predicted linear IgE epitopes. These findings will advance more reliable diagnosis and management of potentially severe food allergy due to crustaceans. PMID:23840718

  13. FLP-FRT-based method to obtain unmarked deletions of CHU_3237 (porU) and large genomic fragments of Cytophaga hutchinsonii.

    PubMed

    Wang, Ying; Wang, Zhiquan; Cao, Jing; Guan, Zhiwei; Lu, Xuemei

    2014-10-01

    Cytophaga hutchinsonii is a widely distributed cellulolytic bacterium in the phylum Bacteroidetes. It can digest crystalline cellulose rapidly without free cellulases or cellulosomes. The mechanism of its cellulose utilization remains a mystery. We developed an efficient method based on a linear DNA double-crossover and FLP-FRT recombination system to obtain unmarked deletions of both single genes and large genomic fragments in C. hutchinsonii. Unmarked deletion of CHU_3237 (porU), an ortholog of the C-terminal signal peptidase of a type IX secretion system (T9SS), resulted in defects in colony spreading, cellulose degradation, and protein secretion, indicating that it is a component of the T9SS and that T9SS plays an important role in cellulose degradation by C. hutchinsonii. Furthermore, deletions of four large genomic fragments were obtained using our method, and the sizes of the excised fragments varied from 9 to 19 kb, spanning from 6 to 22 genes. The customized FLP-FRT method provides an efficient tool for more rapid progress in the cellulose degradation mechanism and other physiological aspects of C. hutchinsonii. PMID:25063660

  14. Una cinta transportadora de longitud l se mueve con velocidad v respecto al suelo. Por su extremo derecho se introduce un bloque de masa m que se mover en contra de la cinta

    E-print Network

    Chang, Mark J. L.

    Problema 2 Una cinta transportadora de longitud l se mueve con velocidad v respecto al suelo. Por). Determine la velocidad vo (respecto al suelo) con la cual es necesario impulsar el bloque contra el, y que ahí se detenga respecto al suelo. En tal punto, con el bloque en reposo respecto al suelo

  15. Una cinta transportadora de longitud l se mueve con velocidad v respecto al suelo. Por su extremo derecho se introduce un bloque de masa m que se mover en contra de la cinta

    E-print Network

    Chang, Mark J. L.

    Problema 2 Una cinta transportadora de longitud l se mueve con velocidad v respecto al suelo. Por su extremo derecho se introduce un bloque de masa m que se moverá en contra de la cinta (ver figura cantidad de calor liberada en el proceso de frenado sobre la cinta. (En la situación planteada se cumple

  16. Antimicrobial Susceptibility as a Diagnostic Aid in the Identification of Nonfermenting Gram-Negative Bacteria

    PubMed Central

    Gilardi, G. L.

    1971-01-01

    Antimicrobial susceptibility data regarding nonfermentative, gram-negative bacteria (Pseudomonas, Alcaligenes, Acinetobacter, Moraxella, Flavobacterium) are presented showing that the antibiograms of most species examined can be used as an important auxillary aid in their differentiation. PMID:5132093

  17. Síndrome del Outlet Torácico: ¿Una Patología Siempre Quirúrgica? Análisis de una Serie de 31 Cirugías Realizadas por Vía Supraclavicular Serie clínica

    PubMed Central

    Socolovsky, Mariano; Di Masi, Gilda; Binaghi, Daniela; Campero, Álvaro; Páez, Miguel Domínguez; Dubrovsky, Alberto

    2014-01-01

    Introducción: El síndrome de outlet torácico es una compresión del plexo braquial que suscita polémica. Se clasifica en Outlet Torácico Verdadero o neurogénico (OTV) y Outlet Torácico Disputado o no neurogénico (OTD). El primero presenta síntomas motores en la mano, mientras que el segundo sólo síntomas sensitivos en el miembro superior. El objetivo de este trabajo es analizar los resultados obtenidos en una serie de 31 cirugías. Métodos: Se analizaron las cirugías de nervios efectuadas entre 2003-2012, tomando los diagnósticos de outlet torácico cuyo período de seguimiento post-operatorio mínimo fuera de 6 meses. Se buscaron los siguientes datos: edad, sexo, presencia de síntomas sensitivos y/o motores, clasificación, resultado de los estudios neurofisiológicos y de imágenes, resultado de la cirugía, complicaciones post-operatorias y recidivas. Resultados: Se incluyeron 31 cirugías realizadas en 30 pacientes, 9 OTV (8 mujeres) de 24.3 años, y 21 con OTD (18 mujeres) de 37.4 años de edad en promedio. Un 90% presentaron alteraciones neurofisiológicas preoperatorias, y 66,6% imagenológicas. En el intraoperatorio, el 100% de los OTV presentó una alteración anatómica relacionada con la sintomatología, hecho observado sólo en el 36.7% de los OTD operados. El 87,5% de los OTV mejoraron sensitivamente, mientras que 77,7% mejoraron la atrofia. Por el contrario, 45.4% de los OTD mejoraron permanentemente, 36.3% no tuvieron cambios, 13.6% mejoraron transitoriamente y 4.5% (un caso) empeoró. Las complicaciones post-operatorias fueron más frecuentes aunque transitorias en el grupo de OTV (3 casos sobre 9 operados, 33.3%) que en los OTD (3 casos sobre 22, un 13.6%). Conclusión: El OTV suele mayormente mejorar luego de la cirugía, igual que el OTD aunque en una proporción mucho menor. Estos hallazgos coinciden con otros reportes recientes de esta patología. PMID:25165614

  18. De conformidad con la Ley de Derechos Humanos de 1977 del Distrito de Columbia, segn enmendada, Cdigo Oficial del Distrito de Columbia, Seccin 2-1401.01 et seq., (Ley), el Distrito de Columbia no discrimina, de forma visible ni percibida, por

    E-print Network

    De conformidad con la Ley de Derechos Humanos de 1977 del Distrito de Columbia, según enmendada la ausencia, el empleado no perderá ninguno de sus beneficios de empleo, ni tampoco los derechos por las estipuladas por la Ley. LAS QUEJAS CON RESPECTO A ALGUNA DENEGACIÓN DE LOS DERECHOS EN VIRTUD DE

  19. The Understanding of Astronomy Concepts by Students from Basic Education of a Public School. (Spanish Title: El Entendimiento de Conceptos de Aastronmía Por Los Alumnos de Educación Básica en Una Escuela Pública.) O Entendimento de Conceitos de Astronomia Por Alunos da Educação Básica: O Caso de Uma Escola Pública Brasileira

    NASA Astrophysics Data System (ADS)

    Iria Machado, Daniel; dos Santos, Carlos

    2011-07-01

    We present the results obtained in a research on the comprehension of basic astronomical concepts, in which 561 students from fifth grade middle school to third grade high school of a public school of the city of Foz do Iguaçu (Brazil) took part. A test with 20 multiple-choice questions was applied to indentify the most common conceptions expressed by the students. This test was elaborated based on the literature about misconceptions and covered the following topics: the day-night cycle; the time zones; the seasons of the year; the phases of the Moon; the movement of the Moon; the apparent movement of the Sun in the celestial sphere; the eclipses; the dimensions and distances in the Universe; the brightness of the stars and its observation from Earth. Though a small progress was verified in the proportion of scientifically acceptable answers when comparing the eighth grade of middle school to the fifth, and the third grade of high school to the first, there was an overall predominance of alternative conceptions regarding most of the explored subjects, which persisted up to the last year of secondary school. The comparison to data found in this research made in other socio-cultural contexts revealed, in many aspects, similar notions and difficulties revealed by the students. Se presentan los resultados de una investigación sobre la comprensión de conceptos astronómicos básicos, en la cual participaron 561 estudiantes que cursaban entre el quinto grado de la enseñanza primaria y el tercer año de la enseñanza secundaria de una escuela pública de la ciudad de Foz do Iguaçu (Brasil). Se utilizó un test de 20 preguntas de opción múltiple para identificar las concepciones más comunes expresadas por los estudiantes. Este instrumento de recolección de datos se desarrolló en base a la literatura sobre las concepciones alternativas y trató los siguientes temas: el ciclo día-noche, los husos horarios, las estaciones del año, las fases de la Luna, el movimiento de la Luna, el movimiento aparente del Sol sobre la esfera celeste, los eclipses, las dimensiones y las distancias en el Universo, el brillo de las estrellas y su observación de la Tierra. Si bien ha habido un pequeño progreso en la proporción de respuestas aceptables científicamente cuando se cotejó el octavo grado de la enseñanza primaria con el quinto, y el último año de la enseñanza secundaria con el primero, se observó un predominio de las concepciones alternativas en relación a la mayoría de los temas explorados, que continuó hasta los últimos años de la educación básica. Una comparación con los datos encontrados en investigaciones realizadas en otros contextos socioculturales revelaron, en muchos aspectos, conceptos y dificultades similares expresadas por los estudiantes. Apresentam-se os resultados de uma investigação sobre a compreensão de conceitos astronômicos básicos, da qual participaram 561 estudantes da quinta série do Ensino Fundamental ao terceiro ano do Ensino Médio de uma escola pública da cidade de Foz do Iguaçu. Um teste com 20 questões de múltipla escolha foi aplicado para identificar as concepções mais comuns expressadas pelos alunos. Esse instrumento de coleta de dados foi elaborado com base na literatura sobre concepções alternativas e abordou os seguintes temas: o ciclo dia-noite; os fusos horários; as estações do ano; as fases da Lua; o movimento da Lua; o movimento aparente do Sol na esfera celeste; os eclipses; as dimensões e distâncias no Universo; o brilho das estrelas e sua observação da Terra. Embora se tenha verificado um pequeno progresso na proporção de respostas cientificamente aceitáveis ao se cotejar a oitava série do Ensino Fundamental com a quinta, e a terceira série do Ensino Médio com a primeira, houve o predomínio de concepções alternativas em relação à maior parte dos temas explorados, as quais persistiram até o último ano da Educação Básica. A comparação com dados encontrados em investigações realizadas em outros contextos socioculturais revelaram, em m

  20. Por qu es tan importante mantenerse activo?

    E-print Network

    Rau, Don C.

    divertirte. Haz del ejercicio parte de tu rutina diaria. Hazlo con algún amigo o miembro de la familia. Es ¿Cómo pueden ayudarme mi familia y amigos? A la hora de realizar cualquier actividad, pídele a tu familia o amistades que te acompañen. Es bueno para todos y ayuda a eliminar el estrés. También es una

  1. Spectra/Por Easy-to-Use

    E-print Network

    Lebendiker, Mario

    and Shelf Life . . . . . . . .10 Membrane Chemical Compatibility . . . . . . . . . . . . . . . .10 Spectra to electrophoresis, HPLC: removing salts such as sodium chloride, detergents, ammonium sulfate, ces

  2. New Energy Harvesting Technologies Por European Editors

    E-print Network

    Chiao, Jung-Chih

    in MEMS, and how designers can harness the generated power. The article will feature devices from Linear technology that provides the latest accelerometers in mobile phones. A research associate and electrical/7/2014 10:57 PM #12;Figure 2: A MEMS windmill to power a cellphone. Smitha Rao and Prof J.C. Chiao designed

  3. Characterization of invasive Neisseria meningitidis from Atlantic Canada, 2009 to 2013: With special reference to the nonpolysaccharide vaccine targets (PorA, factor H binding protein, Neisseria heparin-binding antigen and Neisseria adhesin A)

    PubMed Central

    Tsang, Raymond SW; Law, Dennis KS; Gad, Rita R; Mailman, Tim; German, Gregory; Needle, Robert

    2015-01-01

    BACKGROUND: Serogroup B Neisseria meningitidis (MenB) has always been a major cause of invasive meningococcal disease (IMD) in Canada. With the successful implementation of a meningitis C conjugate vaccine, the majority of IMD in Canada is now caused by MenB. OBJECTIVE: To investigate IMD case isolates in Atlantic Canada from 2009 to 2013. Data were analyzed to determine the potential coverage of the newly licensed MenB vaccine. METHODS: Serogroup, serotype and serosubtype antigens were determined from IMD case isolates. Clonal analysis was performed using multilocus sequence typing. The protein-based vaccine antigen genes were sequenced and the predicted peptides were investigated. RESULTS: The majority of the IMD isolates were MenB (82.5%, 33 of 40) and, in particular, sequence type (ST)-154 B:4:P1.4 was responsible for 47.5% (19 of 40) of all IMD case isolates in Atlantic Canada. Isolates of this clone expressed the PorA antigen P1.4 and possessed the nhba genes encoding for Neisseria heparin-binding antigen peptide 2, which together matched exactly with two of the four components of the new four-component meningococcal B vaccine. Nineteen MenB isolates had two antigenic matches, another five MenB and one meningitis Y isolate had one antigenic match. This provided 75.8% (25 of 33) potential coverage for MenB, or a 62.5% (25 of 40) overall potential coverage for IMD. CONCLUSION: From 2009 to 2013, IMD in Atlantic Canada was mainly caused by MenB and, in particular, the B:4:P1.4 ST-154 clone, which accounted for 47.5% of all IMD case isolates. The new four-component meningococcal B vaccine appeared to offer adequate coverage against MenB in Atlantic Canada.

  4. [Pathogenesis of bacterial respiratory infection and new approach of the treatment].

    PubMed

    Matsumoto, K

    1990-10-01

    Causative agents of respiratory infections has been changed because of increase in number of aged people and compromised host and the rapid development of new chemotherapeutic agents. Especially Branhamella catarrhalis (B. catarrhalis), which is very unique and has become a common respiratory pathogen, since 1980, in my department. Attachment ability of B. catarrhalis to oropharyngeal cells coincided with the acute exacerbation of chronic respiratory infections by this bacterium and the same phenomenon in pneumococcal infections was also established. In the hospital for aged people, two major pathogens Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) are specially seen. In these patients, the two major ones were isolated from the oropharynx. Non-typable Haemophilus influenzae (H. influenzae) is also very important in Japan like USA. Recurrent infection with this pathogen occurred due to the change of OMPs during the time period of more than one month. Complement and some amount of ceftadizim were inactivated by destroyed neutrophils in vitro. This result may explain one of the cause of intractability of P. aeruginosa infection. Monoclonal antibody against P. aeruginosa showed effectiveness in P. aeruginosa pneumonia model in mice. Intraabdominal administration of fibronectin also was effective for staphylococcal pneumonia in rat. Oropharyngeal pathogens like S. aureus, S. pneumonia, B. catarrhalis, H. influenzae and P. aeruginosa were killed by 100-500 times diluted solution of 7% povidonjod solution. Moreover the frequency of recurrence of infection by these bacteria were decreased by gargling this solution 3-4 times/day.(ABSTRACT TRUNCATED AT 250 WORDS) PMID:2125670

  5. Biosensors and Bioelectronics 21 (2005) 523527 Short communication

    E-print Network

    Chen, Wilfred

    2005-01-01

    Biosensors and Bioelectronics 21 (2005) 523­527 Short communication Amperometric microbial biosensor for p-nitrophenol using Moraxella sp.-modified carbon paste electrode Priti Mulchandani, Carlos M microbial biosensor for highly specific, sensitive and rapid quantitative determination of p

  6. New halide-centered discrete Ag(I)(8) cubic clusters containing diselenophosphate ligands, [Ag(8)(X)[Se(2)P(OR)(2)](6)](PF(6)) (X = Cl, Br; R = Et, Pr, (i)Pr): syntheses, structures, and DFT calculations.

    PubMed

    Liu, C W; Haia, Hsien-Chung; Hung, Chiu-Mine; Santra, Bidyut Kumar; Liaw, Ben-Jie; Lin, Zhenyang; Wang, Ju-Chun

    2004-07-12

    Six clusters Ag(8)(micro(8)-X)[Se(2)P(OR)(2)](6)(PF(6)) (R = Et, X = Cl, 1a, X = Br, 1b; R = Pr, X = Cl, 2a, X = Br, 2b; R = (i)Pr, X = Cl, 3a, X = Br, 3b) were isolated from the reaction of [Ag(CH(3)CN)(4)](PF(6)), NH(4)[Se(2)P(OR)(2)], and Bu(4)NX in a molar ratio of 4:3:1 in CH(2)X(2). Positive FAB mass spectra show m/z peaks at 2573.2 for 1a, 2617.3 for 1b, 2740.9 for 2a, 2786.9 for 2b, 2742.3 for 3a, and 2787.0 for 3b due to respective molecular cation, (M - PF(6))(+). (31)P NMR spectra of 1a-3b display a singlet at delta 82.3, 81.5, 82.9, 81.7, 76.3, and 75.8 ppm with a set of satellites (J(PSe) = 661, 664, 652, 652, 656, and 656 Hz, respectively). The X-ray structure (1a-2b) consists of a discrete cationic cluster in which eight silver ions are linked by six diselenophosphate ligands and a central micro(8)-Cl or micro(8)-Br ion with a noncoordinating PF(6)(-) anion. The shape of the molecule is a halide-centered distorted Ag(8) cubic cluster. The dsep ligand exhibits a tetrametallic tetraconnective (micro(2), micro(2)) coordination pattern, and each caps on a square face of the cube. Each silver atom of the cube is coordinated by three selenium atoms and the central chloride or bromide ion. Additionally, molecular orbital calculations at the B3LYP level of the density functional theory have been carried out to study the Ag-micro(8)-X (X = Cl, Br) interactions for cluster cations [Ag(8)(micro(8)-X)[Se(2)P(OR)(2)](6)](+). Calculations show very weak bonding interactions exist between micro(8)-X and Ag atoms of the cube. PMID:15236560

  7. Production of UC-labeled gas in BACTEC Neisseria Differentiation kits by Neisseria cinerea

    SciTech Connect

    Boyce, J.M.; Mitchell, E.B. Jr.; Knapp, J.S.; Buttke, T.M.

    1985-09-01

    Six strains of Neisseria cinerea were tested in BACTEC Neisseria Differentiation kits (Johnston Laboratories, Inc., Towson, Md.), and all yielded positive glucose growth indices and negative maltose and fructose growth indices. These results were similar to those achieved with Neisseria gonorrhoeae. However, most of the N. cinerea isolates tested yielded 3-h glucose growth indices that were lower than those obtained with gonococci. UC-labeled gas was produced significantly faster by N. gonorrhoeae than by N. cinerea. Additional studies suggested that the UC-labeled gas produced by N. cinerea was carbon dioxide. N. cinerea strains were similar to Branhamella catarrhalis strains because both species failed to produce detectable acid from glucose, maltose, sucrose, fructose, and lactose in cysteine-tryptic agar media. However, in contrast to N. cinerea strains, B. catarrhalis strains did not metabolize glucose in BACTEC Neisseria Differentiation kits.

  8. UNIVERSIDAD DE ALICANTE ASIGNACIN BECAS-COLABORACIN POR DEPARTAMENTOS

    E-print Network

    Escolano, Francisco

    Construcciones Arquitectónicas 1 Derecho Civil 1 Derecho del Trabajo y de la Seguridad Social 1 Derecho Internacional Público y Derecho Penal 1 Derecho Mercantil y Derecho Procesal 1 Didáctica General y Didácticas Filologías Integradas 1 Filosofía del Derecho y Derecho Internacional 1 Física Aplicada 1 Física, Ingeniería

  9. Enfermedad diarreica aguda por Escherichia coli patógenas en Colombia

    PubMed Central

    Gómez-Duarte, Oscar G.

    2014-01-01

    Resumen Las cepas de E. coli patógenas intestinales son causas importantes de la enfermedad diarreica aguda (EDA) en niños menores de 5 años en América Latina, África y Asia y están asociadas a alta mortalidad en niños en las comunidades más pobres de África y el Sudeste Asiático. Estudios sobre el papel de las E. coli patógenas intestinales en la EDA infantil en Colombia y otros países de América Latina son limitados debido a la carencia de ensayos para detección de estos patógenos en los laboratorios clínicos de centros de salud. Estudios recientes han reportado la detección de E. coli patógenas intestinales en Colombia, siendo la E. coli enterotoxigénica la cepa más frecuentemente asociada a diarrea en niños menores de 5 años. Otros patógenos detectados en estos pacientes incluyen las E. coli enteroagregativa, enteropatógena, productora de toxina Shiga, y de adherencia difusa. Con base en estudios que reportan la presencia de E. coli productora de toxina Shiga y E. coli enteroagregativa en carnes y vegetales en supermercados, se cree que productos alimentarios contaminados contribuyen a la transmisión de estos patógenos y a la infección del huésped susceptible. Más estudios son necesarios para evaluar los mecanismos de transmisión, el impacto en la epidemiologia de la EDA, y las pautas de manejo y prevención de estos patógenos que afectan la población pediátrica en Colombia. PMID:25491457

  10. Los servicios bibliotecarios estn integrados por: -Coordinacin General de Servicios

    E-print Network

    . COORDINACIÓN GENERAL DE SERVICIOS BIBLIOGRÁFICOS Unidad de Servicios Bibliográficos Lic. Alberto F. Zurita Gómez Area de Sistemas de Información Lic. Ma. Elena Luna Morales Lic. J. Enrique Montaño Molina Raúl Cuevas Córdova Area Especializada de Análisis Bibliométrico Lic. Marco L. Muñoz Rivera Lic. Sergio Rangel

  11. Evolución de una protuberancia observada por el HASTA

    NASA Astrophysics Data System (ADS)

    Luoni, M. L.; Francile, C.

    Prominence eruptions are one of the most spectacular manifestations of solar activity; in addition to flares and coronal mass ejections. Both filaments and prominences are chromospheric material suspended in the corona by the magnetic field. Hence their importance as tracers of field ejections. Some parts of their magnetic structure are not well understood; especially with regard to the loss of stability. On 06 December 2010 the H-Alpha Telescope for Argentina (HASTA) observed a prominence in the eastern solar limb including when part of it was ejected. In this paper the evolution of the filament is analyzed; its structure before and after the eruption; determining parameters that characterize it from HASTA data. FULL TEXT IN SPANISH

  12. Camina por Salud: Walking in Mexican-American Women

    PubMed Central

    Keller, Colleen S.; Gonzales, Adelita

    2008-01-01

    Forty-six percent of older Mexican-American women report no leisure time physical activity (PA); 38.1% are obese. This study (1) evaluated a PA intervention on reduction of risk for coronary heart disease (CHD) and (2) determined which variables affected adherence to PA. For 36 weeks, Group I walked 3 days/week; Group II walked 5 days/week. The investigators measured total body fat, regional fat, blood lipids, and adherence to PA The walking interventions favorably affected body fat, with significant differences in body mass index (BMI) reductions[F (2, 16) = 12.86, p = .001]. No statistical differences were noted in the anthropometric and blood lipid results from baseline to the 36-week measures. PMID:18457751

  13. Por: Christa von Hillebrandt-Andrade Investigadora Auxiliar y Directora

    E-print Network

    Gilbes, Fernando

    .html #12;Recuperación #12;Imágenes de Quickbird (Digital Globe) de la ciudad de Boumerdes, Algeria Antes (4/22/02) Después (5/23/03) Terremoto de Algeria, Mayo 21, 2003, M 6.8 (USGS, 2003) #12;Terremoto Bam,Iran Dec. 26

  14. GrGrfico de Controle por Atributosfico de Controle por Atributos VVctorctor HugoHugo LachosLachos DDvilavila

    E-print Network

    Lachos, Victor

    número observado de itens não conformes. Uma estimativa da fração não conforme é : n pp pLSC pLM n pp p,0 )1( 3 05,0 096,0 25 95,005,0 305,0 )1( 3 = × -= - -= == = × += - += n pp pLSC pLM n pp pLSC #12 controle para fração não conforme: i i n pp pLIC pLM n pp pLSC )1( 3 )1( 3 - -= = - += = = = m i i m i i

  15. Comparative study of the aerobic, heterotrophic bacterial flora of Chesapeake Bay and Tokyo Bay.

    PubMed Central

    Austin, B; Garges, S; Conrad, B; Harding, E E; Colwell, R R; Simidu, U; Taga, N

    1979-01-01

    A comparative study of the bacterial flora of the water of Chesapeake Bay and Tokyo Bay was undertaken to assess similarities and differences between the autochthonous flora of the two geographical sites and to test the hypothesis that, given similarities in environmental parameters, similar bacterial populations will be found, despite extreme geographic distance between locations. A total of 195 aerobic, heterotrophic bacterial strains isolated from Chesapeake Bay and Tokyo Bay water were examined for 115 biochemical, cultural, morphological, nutritional, and physiological characters. The data were analyzed by the methods of numerical taxonomy. From sorted similarity matrices, 77% of the isolates could be grouped into 30 phena and presumptively identified as Acinetobacter-Moraxella, Caulobacter, coryneforms, Pseudomonas, and Vibrio spp. Vibrio and Acinetobacter species were found to be common in the estuarine waters of Chesapeake Bay, whereas Acinetobacter-Moraxella and Caulobacter predominated in Tokyo Bay waters, at the sites sampled in the study. PMID:453838

  16. THE BACTERIOLOGY OF SPUTUM IN COMMON NON-TUBERCULOUS INFECTIONS OF THE UPPER AND LOWER RESPIRATORY TRACTS, WITH SPECIAL REFERENCE TO LOBAR AND BRONCHO-PNEUMONIA

    PubMed Central

    Hastings, Thomas Wood; Niles, Walter L.

    1911-01-01

    1. In our examinations, only 38 per cent. of the infections of the respiratory tract below the glottis were pure, and this percentage was reached only by carefully following Kitasato's method of handling sputa. 2. Lobar pneumonia may produce sputum free from pneumococci, and may undoubtedly be caused by organisms other than thepneumococcus. 3. There is found a marked yearly variation in the organisms which excite inflammation of the respiratory tract. 4. Micrococcus catarrhalis is usually considered a common secondary invader; but it may, and probably frequently does, assume pathogenic properties. PMID:19867447

  17. ESTUDIO DE LA CINESTUDIO DE LA CINTICA DE LA REACCITICA DE LA REACCINN DE OXIDACIDE OXIDACIN DE PIRITA POR REDUCCIN DE PIRITA POR REDUCCINN

    E-print Network

    Politècnica de Catalunya, Universitat

    ++ +++++ 2HFe2SO7NOHFeS7NO 2-2 4 - 222 - 3 O 8HH)2Fe(4SO15NO7H2FeS15NO 3 -2 4 - 222 - 3 + +++++ OO S tot, Fe2+ NO3/SO4 = 1.4 S tot, SEM Fe(OH)3 NO3/SO4 = 1.5 S tot, Fe2+, NO2 - NO3/SO4= 3.5 S tot, NO2 -, SEM Fe

  18. Influence of outdoor winter environment on the course of infectious bovine keratoconjunctivitis

    SciTech Connect

    Kopecky, K.E.; Pugh, G.W. Jr.; McDonald, T.J.

    1981-11-01

    The effect of environmental conditions on the onset, severity, and duration of Moraxella bovis infection and subsequent clinical disease was studied. Twelve calves were used; 6 were maintained under usual isolated experimental conditions (inside), and 6 were kept under normal feedlot conditions (outside) during the winter (-20 to +15 C). The cattle housed inside had a higher infection rate, a milder disease, and longer duration of infection than did the cattle kept out side. Seemingly, the stress of the cold weather caused a more severe disease of shorter duration.

  19. Aerobic bacterial flora of oral and nasal fluids of canines with reference to bacteria associated with bites.

    PubMed Central

    Bailie, W E; Stowe, E C; Schmitt, A M

    1978-01-01

    Oral and nasal fluids of 50 dogs were examined to determine the prevalence of aerobic bacteria frequently associated with animal bite wounds. The most frequently isolated microorganisms included: IIj, EF-4, Pasteurella multocida, Staphylococcus aureus, Staphylococcus epidermidis, group D streptococci, Corynebacterium sp., Enterobacteria, Neisseria sp., Moraxella sp., and Bacillus sp. Other species and genera were infrequently recovered and may represent transient flora. The high incidence of IIj, EF-4, P. multocida, and S. aureus, all known human pathogens, suggests that they should be considered as probably contaminants in bite wounds. Images PMID:632349

  20. Wavelength of ultraviolet radiation that enhances onset of clinical infectious bovine keratoconjunctivitis

    SciTech Connect

    Kopecky, K.E.; Pugh, G.W. Jr.; Hughes, D.E.

    1980-09-01

    Cellulose acetate filtered ultraviolet (uv) radiation and unfiltered uv radiation were used on calves that were subsequently challenge exposed with Moraxella bovis. The onset, course, and severity of infectious bovine keratoconjunctivitis (IBK) were studied. Ten calves irradiated with unfiltered uv had the disease 1 to 2 days after M bovis challenge exposure. Ten calves irradiated with filtered uv and 10 calves not irradiated manifested IBK in a similar manner. Evidence is presented to support the contention that the wavelengths (around 270 nm) which are eliminated by cellulose acetate enhance the course of IBK. The effects on IBK of environmentally increased solar uv radiation is also discussed.

  1. Culturable Aerobic and Facultative Anaerobic Intestinal Bacterial Flora of Black Cobra (Naja naja karachiensis) in Southern Pakistan

    PubMed Central

    Iqbal, Junaid; Sagheer, Mehwish; Tabassum, Nazneen; Siddiqui, Ruqaiyyah; Khan, Naveed Ahmed

    2014-01-01

    Using morphological analysis and biochemical testing, here for the first time, we determined the culturable gut bacterial flora (aerobes and facultative anaerobes) in the venomous Black Cobra (Naja naja karachiensis) from South Asia. The findings revealed that these snakes inhabit potentially pathogenic bacteria including Serratia marcescens, Pseudomonas aeruginosa, Shewanella putrefaciens, Aeromonas hydrophila, Salmonella sp., Moraxella sp., Bacillus sp., Ochrobactrum anthropi, and Providencia rettgeri. These findings are of concern, as injury from snake bite can result in wound infections and tissue necrosis leading to sepsis/necrotizing fasciitis and/or expose consumers of snake meat/medicine in the community to infections. PMID:25002979

  2. Advanced Decontamination Technologies: Irradiation

    NASA Astrophysics Data System (ADS)

    Lee, Eun Joo; Ahn, Dong U.

    Bacterial food-borne illnesses account for an estimated 76 million cases, 325,000 hospitalizations, and 5,000 deaths each year in the United States (CDCP, 2005), and 5,300 food-borne outbreaks in Europe resulted in 5,330 hospitalizations and 24 deaths in 2005 (Aymerich, Picouet, & Monfort, 2008). Major food-borne pathogens of concern include Escherichia coli O157:H7, Campylobacter jejuni/coli, Salmonella spp., Listeria monocytogenes, Clostridium botulinum/perfringens, Staphylococcus aureus, Aeromonas hydrophylia, and Bacillus cereu, and spoilage microorganisms include Pseudomonas, Acinetobacter/Moraxella, Aeromonas, Alteromonas putrefaciens, Lactobacillus, and Brochothrix thermosphecta (Mead et al., 1999).

  3. Doctrina cristiana para instrucción e información de los indios, por manera de hystoria 

    E-print Network

    Có rdoba, Pedro de, 1482-1521 Doctrina cristiana para instrucció n e informació n de los indios, por manera de hystoria  Có rdoba, Pedro de, 1482-1521 Los Suelos y la agricultura en el municipio de Artemisa  Banco de Fomento Agrí cola e Industrial de Cuba. Division Agrí cola. Departamento de Diversificací on

    1957-01-01

    on the available energy concept, and it is Each has its own merits in shedding light on shown that improvement of the process system is where and how energy is used. Some engineering deci evolutionally carried out as the result of .the de sions for process... by the available energy balance. The first law cannot reflect the loss of available energy because energy is always conserved. The second law reflects the loss associated with a change of gr.de of an energy quantity. Unlike energy, the avail~ble energy...

  4. 22 BULLETIN OF THE UNITED STATES FISH COMMISSION. LIST O F WATER PLANTS POR CARP PONDS.

    E-print Network

    common in Europe. ONAGRACEB. Evening Primrose family. UMBELLIFERB. Trapa natans, L. Europe, Siberia latifolia, Willd.).- Europe, Siberia. Arrowhead. Common. Europej Northern Asia, Butomus untbeltatus, L COMMISSION. 25 IRIDACEB. Iris family. lris pseudacorus, L. Europe ; Siberia. JUNCACEBI. Rush family. Juncus

  5. Renovando la Esperanza por una Educacion sin Exclusiones (Rekindling the Hope for an Education without Exclusion).

    ERIC Educational Resources Information Center

    Revista Interamericana de Educacion de Adultos, 2001

    2001-01-01

    Articles in this issue, written in Spanish, focus on the following: current status and outlook of youth and adult education; opening statement of the 50th anniversary commemoration; regional framework for the education of youth and adults in Latin America and the Caribbean; interculturalism and the education of youth and adults; participation of…

  6. Por el desarrollo libre del Espritu 1 DIPLOMADO INTERVENCIN EN BULLYING Y MEDIACIN ESCOLAR.

    E-print Network

    Pérez, Carlos E.

    mismos compañeros y compañeras de escuela. Esta situación ha generado gran preocupación entre profesores, administrativos directivos y padres de familia, quienes se sienten con herramientas limitadas para enfrentar este obtenidos. Un estudio de UNICEF en el año 2004, muestra que la discriminación en la escuela (ser rechazado

  7. Doctrina cristiana para instrucción e información de los indios, por manera de hystoria 

    E-print Network

    Có rdoba, Pedro de, 1482-1521

    2011-03-25

    The influence of peers and peer norms is a significant health determinant of adolescent sexual activity, yet little is known in health education about differences between peer pressure and friend pressure on adolescents. The objective of this study...

  8. y por conservacin de la biodiver-sidad, que beneficiar hasta 2008

    E-print Network

    Sarkar, Sahotra

    recar- ga y retención de suelos, lo que ha significado una amplia derrama económica para los, relacionada con el acelerado cambio de uso de suelo, impacta negativamente la permanencia de la biodiversidad

  9. The Escuela Moderna Movement of Francisco Ferrer: "Por la Verdad y la Justicia."

    ERIC Educational Resources Information Center

    Fidler, Geoffrey C.

    1985-01-01

    The educational theory and practice of the Escuela Modern (Modern School) movement of the Spanish educator Francisco Ferrer, born in 1850, are discussed. Two fundamental tendencies of the movement are child-centered education and education in didactic terms. (RM)

  10. Diplomaturas y Licenciaturas por facultades y escuelas en Centros Propios de la UAM TITULACIONES (ESTUDIOS)

    E-print Network

    Autonoma de Madrid, Universidad

    Técnicas de Mercado Turismo Economía DERECHO Derecho Ciencias Políticas y de la Administración Derecho / Administración y Dirección de Empresas Derecho / Ciencias Políticas y de la Aministración ESCUELA POLITÉCNICA Económica Aplicada Nueva Economía Mundial Derecho Mercantil Derecho Privado Patrimonial Derechos

  11. Comienza la construcción de instalación patrocinada por el NCI en Puerto Rico para realizar estudios

    Cancer.gov

    El gobierno de Puerto Rico ha destinado $196 millones de dólares para construir un hospital oncológico de 287 000 pies cuadrados en San Juan, que contará con 96 camas. El nuevo hospital es el primero en su clase en la región caribeña y en él se llevarán a

  12. PREJUICIO Y DISTANCIA SOCIAL HACIA PERSONAS HOMOSEXUALES POR PARTE DE JÓVENES UNIVERSITARIOS

    PubMed Central

    Fernández Rodríguez, María del C.; Squiabro, José Calderón

    2014-01-01

    Se realizó un estudio descriptivo transversal con el propósito de explorar actitudes de rechazo y distancia social hacia las personas gays y lesbianas (GL) en 565 universitarios. Se utilizó una escala para medir Prejuicio y otra escala para medir Distancia Social. Los participantes reflejaron niveles moderados de prejuicio y distancia social (DS) hacia las personas gays y lesbianas. Los varones (M=104.5, DT= 27.47) mostraron significativamente más prejuicio que las mujeres (M=98.8, DT= 23.41). Los hombres (M=22.7, DT= 7.00) mostraron significativamente mayor DS que las mujeres (M=21.1, DT= 5.41). Las personas que asisten con regularidad a la iglesia mostraron más prejuicio y DS que los que no asisten. Se analiza importancia de incluir el tema de la diversidad sexual a través del currículo para desmontar prejuicios hacia la comunidad homosexual. PMID:25606066

  13. Looking for a Job: Step by Step = Buscando Trabajo: Paso por Paso.

    ERIC Educational Resources Information Center

    Edwards, Patricia

    This bilingual document provides guidelines and learning activities to assist migrant workers in looking for a job. The document covers the following areas: (1) a checklist providing an overview of job search skills; (2) developing a fact sheet of personal information; (3) listing good work qualities; (4) identifying references and securing…

  14. Language and La Academia, If English Works, Por Que Se Emplea Espanol?

    ERIC Educational Resources Information Center

    Jonz, Jon G.

    1978-01-01

    The dynamics of Mexican American nationalism reflected in the publications of the Academia de la Nueva Raza and in a 1971 work by Ricardo Sanchez are examined in this article. The connection between language and ideology is discussed in the context of Chicano nationalist writing. (GC)

  15. 508 Mastozoologa Neotropical, 2009 por la presencia del bosque nativo y la proteccin

    E-print Network

    Nacional Autónoma de México, Universidad

    , y una menor tolerancia a los cambios en el paisaje respecto al puma. Mientras las áreas aptas para el jaguar también fueron aptas para el puma, el 54% de las áreas aptas para el puma no fueron consideradas con aptitud para el jaguar, demostrando la mayor resiliencia del puma a la fragmentación y pérdida

  16. ABC 18/06/2004 ESPAA PIERDE POR GOLEADA EN EUROPA

    E-print Network

    Bilbao Arrese, Jesús Mario

    Europea, en el que se eliminan los votos ponderados contemplados en el Tratado de Niza. Este borrador respecto a las reglas incluidas en el Tratado de Niza. Como una imagen vale más que mil palabras, en el

  17. Servicios de Informacin Cientfica y Tcnica Los servicios bibliotecarios estn integrados por

    E-print Network

    . Moreno Armella Unidad de Servicios Bibliográficos Lic. Alberto F. Zurita Gómez Sistemas de Información Lic. Ma. Elena Luna Morales Raúl Cuevas Córdova Unidad Especializada de Análisis Bibliométrico Lic. Marco L. Muñoz Rivera Lic. Sergio Rangel Hernández Adquisiciones y Control Financiero Celia César

  18. Servicios de Informacin Cientfica y Tcnica Los servicios bibliotecarios estn integrados por

    E-print Network

    Servicios Bibliográficos Lic. Alberto F. Zurita Gómez Área de Sistemas de Información Lic. Ma. Elena Luna Morales Lic. J. Enrique Montaño Molina Raúl Cuevas Córdova Área Especializada de Análisis Bibliométrico Lic. Marco L. Muñoz Rivera Lic. Sergio Rangel Hernández Lic. Emma Hernández Gómez Área de

  19. Servicios de Informacin Cientfica y Tcnica Los servicios bibliotecarios estn integrados por

    E-print Network

    . Moreno Armella Unidad de Servicios Bibliográficos Lic. Alberto F. Zurita Gómez Área de Sistemas de Información Lic. Ma. Elena Luna Morales Raúl Cuevas Córdova Área Especializada de Análisis Bibliométrico Lic. Marco L. Muñoz Rivera Lic. Sergio Rangel Hernández Lic. Emma Hernández Gómez Área de Adquisiciones y

  20. Servicios de Informacin Cientfica y Tcnica Los servicios bibliotecarios estn integrados por

    E-print Network

    . Moreno Armella Unidad de Servicios Bibliográficos Lic. Alberto F. Zurita Gómez Sistemas de Información Lic. Ma. Elena Luna Morales Raúl Cuevas Córdova Area Especializada de Análisis Bibliométrico Lic. Marco L. Muñoz Rivera Lic. Sergio Rangel Hernández Lic. Emma Hernández Gómez Adquisiciones y Control

  1. ltima modificacin: 29-04-2014 11673 -TELFIB -Telecomunicacin por Fibra ptica

    E-print Network

    Politècnica de Catalunya, Universitat

    de los componentes de las redes de transporte ópticas, así como su realización. También estudiarán submarinos, así como las arquitecturas de redes ópticas más relevantes: acceso, área metropolitana (MAN previas Comunicaciones ópticas. Sistemas de Telecomunicaciones. Requisitos #12;Última modificación: 29

  2. Home before You Know It = De regres en casa en un dos por tres.

    ERIC Educational Resources Information Center

    Vida Health Communications, Inc., Cambridge, MA.

    The arrival of a newborn requires a great deal of adjustment. Intended for new and expectant parents, this booklet and companion video provide practical advice and hands-on demonstrations of the essentials of mother and baby care, from birth to the first visit to the pediatrician. The first part of the booklet, which comes in both English- and…

  3. CONVOCATORIA DE MARCHA POR LA CIENCIA Coincidiendo con " La Noche de los investigadores"

    E-print Network

    Fitze, Patrick

    de un capital humano irreemplazable. DATOS: Según el estudio del Área Europea de Investigación impactado de forma determinante en la situación de los recursos humanos, produciéndose una notable reducción conocimiento, acrecentándose así las desigualdades y desperdiciando el talento de las generaciones de

  4. Retórica y negación freudiana en Esta noche juntos, amándonos tanto por Maruxa Vilalta

    E-print Network

    Hoeg, Jerry

    1997-10-01

    , 1982. De Man, Paul. "Semiology and Rhetoric." Diacritics 3.3 (1973): 27-33. Freud, Sigmund. El malestar en la cultura. Madrid: Alianza, 1984. Kristeva, Julia. Semiótica 1. Madrid: Editorial Fundamentos, 2a ed. 1981. Orlando, Francesco. Toward a..., 1982. De Man, Paul. "Semiology and Rhetoric." Diacritics 3.3 (1973): 27-33. Freud, Sigmund. El malestar en la cultura. Madrid: Alianza, 1984. Kristeva, Julia. Semiótica 1. Madrid: Editorial Fundamentos, 2a ed. 1981. Orlando, Francesco. Toward a...

  5. El Titulo IX y La Discriminacion por Sexo (Title IX and Sex Discrimination).

    ERIC Educational Resources Information Center

    Office for Civil Rights (ED), Washington, DC.

    Title IX of the Education Amendments of 1972 protects people from discrimination based on sex in education programs or activities that receive Federal financial assistance. This brochure outlines the responsibilities of education programs and activities covered by Title IX, the responsibilities of the Office for Civil Rights (OCR) in enforcing…

  6. José González Dávila: Réquiem por un amigo que inicia su leyenda

    E-print Network

    Mijares, Enrique

    2000-10-01

    los demás.Esa condición exacerbó su espíritu de voyeur y lo convirtió en el denunciante implacable de la podredumbre social, en el mirón inclemente de los bajos fondos del ser humano, en el inocente que no tiene más remedio que mirar lo que mira...

  7. Luchando por una educacion: A Qualitative Understanding of Undocumented Latina/o College Student Motivation

    ERIC Educational Resources Information Center

    Navarro, Elvia Lorena

    2013-01-01

    The current qualitative study explored the factors and resources that motivate undocumented Latino/a college students to persist in higher education. Through the data obtained from the four qualitative open-ended survey questions, a content analysis revealed specific codes, themes, and subthemes addressing the factors and resources that motivate…

  8. Couer: This image of a por tion of the sky was produced

    E-print Network

    was one of progress and accomplishment for JPL. The Infrared Astronomical Satellite mission was a dramatic the start of the Venus Radar Mapper was approved and the initial design phase of a mission to Mars was also represents an average of the infrared energy emitted in one small region of the sky. The resolution in other

  9. "Por Los Ojos De Madres": Latina Mothers' Understandings of College Readiness

    ERIC Educational Resources Information Center

    Cortez, Laura Jean; Martinez, Melissa Ann; Sáenz, Victor B.

    2014-01-01

    In this study, data from six focus groups with 30 Latina mothers in South Texas were analyzed utilizing a "funds of knowledge" approach to uncover their understandings of college readiness and their role in ensuring their children are college ready. Findings indicate that Latina mothers perceived college readiness in a holistic fashion,…

  10. Emisiones en el interior de las viviendas por la combustión de carbón en los hogares

    Cancer.gov

    Quemar carbón dentro de las viviendas para calefacción o preparación de alimentos produce emisiones de partículas y gases que pueden contener una cantidad de sustancias químicas dañinas, como benceno, monóxido de carbono, formaldehído e hidrocarburo aromático policíclico.

  11. Producao d Dijatos por Dupla Troca de Pomeron Exclusiva no Experimento D0

    SciTech Connect

    Rangel, Murilo, Santana; /Rio de Janeiro, CBPF

    2008-10-01

    The first search for exclusive diffractive dijet production with invariant mass {approx}> 100 GeV in Run II of the Fermilab Tevatron Collider is performed. The set of data used is the Run IIa, corresponding to an integrated luminosity of 30 pb{sup -1} of p{bar p} collisions at {radical}s = 1.96 TeV taken with the D0 detector. At 95% CL, an upper limit for the ratio between the number of diffractive exclusive events and the number of non diffractive events is set to be 7.5 x 10{sup -6}, excluding two of the three models proposed to explain this production.

  12. Association of the NADPH:protochlorophyllide oxidoreductase (POR) with isolated etioplast inner

    E-print Network

    Timko, Michael

    and hydrolysable ATP were vital for the process. After treating the membranes with thermolysin, sodium hydroxide with a paracrystalline membrane structure, the PLB. Keywords: etioplasts, chloroplasts, NADPH the differentiation of proplastids or etio- plasts to chloroplasts. Etiolated angiosperms posses photoreceptors

  13. Inicio Nosotros Enve su Noticia Agencias Miembros de los Medios Buscar Archivos Noticias por Categora Noticias por Pas Noticias por MSA Todas las Noticias de Hoy Buscar Noticias por Da PR TrackbacksTM ViewNewsTM eBook Digests RSS

    E-print Network

    Chiao, Jung-Chih

    Delivery, Biosensores, Microgeneradores de energía, RF MEMS, Nanotecnología, BioMEMS en aplicaciones biológicas reúne Pioneros y Líderes en el campo. Biónica, Biochips, Nanotecnología y MEMS Marketing son solo médicas y biológicas que reunirá Pioneros y Líderes Internacionales en el campo. Biónica, Biochips, Drug

  14. El Libro de la Escritura por Pinguino Tinto (The Writing Book, by Inky Penguin).

    ERIC Educational Resources Information Center

    Padgett, Ron

    Presented completely in Spanish and intended for elementary level students, this book offers 12 writing ideas and several suggestions on how students can make a book using their writing. Each writing idea is presented with a brief description (addressed to the student), several examples of student writing, and a blank page on which to write.…

  15. CICLO DE TALLERES POR EL DA INTERNACIONAL DE LA ELIMINACIN DE LA VIOLENCIA CONTRA LAS

    E-print Network

    Escolano, Francisco

    Formadora en Género. Master en Género y Políticas de Igualdad U.V. FECHAS VALENCIA. Día 29 de Octubre de Noviembre) CONTENIDO - ¿Qué es la violencia de género? Causas - Tipos de violencias contra las mujeres, otros...) - ¿Cómo autocuidarme y autodefenderme frente a la violencia de género? - ¿Qué quiero y que

  16. del 4 al 8 de marzo de 2013 IGUALDADIII Semana por la

    E-print Network

    Fernández Pascual, Ricardo

    el Día Internacional de las Mujeres de la Red de Unidades de Igualdad de Género para la Excelencia de la Mujer (Ministerio de Sanidad, Servicios Sociales e Igualdad). MARTES 5 DE MARZO 11:00 h. Mesa Serna Meroño. Profesora Titular de Derecho Civil y Directora de la Unidad para la Igualdad entre Mujeres

  17. The nasopharyngeal microbiota of feedlot cattle.

    PubMed

    Holman, Devin B; Timsit, Edouard; Alexander, Trevor W

    2015-01-01

    The bovine nasopharyngeal tract plays an important role in animal health and welfare by acting as a site for the carriage of pathogens causing bovine respiratory disease, a condition which results in significant morbidity and mortality in feedlot cattle. We characterized the bacterial nasopharyngeal microbiota in cattle at feedlot entry (day 0) and day 60 using 454 pyrosequencing. We also identified the most frequently isolated aerobic bacteria from nasopharyngeal swabs after plating onto three types of media. The cattle nasopharyngeal microbiota was composed primarily of Proteobacteria (68.9%) and Firmicutes (19.2%). At the genus-level, there was more inter-individual variability and a total of 55 genera were identified. The genera Pseudomonas (23.7%), Shewanella (23.5%), Acinetobacter (17.5%), and Carnobacterium (12.2%) were most prevalent at entry, while after 60 days in the feedlot, Staphylococcus (20.8%), Mycoplasma (14.9%), Mannheimia (10.4%), and Moraxella (9.4%) were dominant. The nasopharyngeal microbiota also became more homogenous after 60 days in the feedlot and differed in structure at day 0 and 60. Using culture-based methods, the most frequently isolated bacteria from nasopharyngeal swabs were Bacillus, Staphylococcus, Moraxella, Pasteurella, and Mannheimia. These results provide insight into the nasopharyngeal microbiota of cattle and demonstrate that specific changes take place during feedlot production. PMID:26497574

  18. The nasopharyngeal microbiota of feedlot cattle

    PubMed Central

    Holman, Devin B.; Timsit, Edouard; Alexander, Trevor W.

    2015-01-01

    The bovine nasopharyngeal tract plays an important role in animal health and welfare by acting as a site for the carriage of pathogens causing bovine respiratory disease, a condition which results in significant morbidity and mortality in feedlot cattle. We characterized the bacterial nasopharyngeal microbiota in cattle at feedlot entry (day 0) and day 60 using 454 pyrosequencing. We also identified the most frequently isolated aerobic bacteria from nasopharyngeal swabs after plating onto three types of media. The cattle nasopharyngeal microbiota was composed primarily of Proteobacteria (68.9%) and Firmicutes (19.2%). At the genus-level, there was more inter-individual variability and a total of 55 genera were identified. The genera Pseudomonas (23.7%), Shewanella (23.5%), Acinetobacter (17.5%), and Carnobacterium (12.2%) were most prevalent at entry, while after 60 days in the feedlot, Staphylococcus (20.8%), Mycoplasma (14.9%), Mannheimia (10.4%), and Moraxella (9.4%) were dominant. The nasopharyngeal microbiota also became more homogenous after 60 days in the feedlot and differed in structure at day 0 and 60. Using culture-based methods, the most frequently isolated bacteria from nasopharyngeal swabs were Bacillus, Staphylococcus, Moraxella, Pasteurella, and Mannheimia. These results provide insight into the nasopharyngeal microbiota of cattle and demonstrate that specific changes take place during feedlot production. PMID:26497574

  19. Diagnostic value of interactions between members of the family Neisseriaceae and lectins.

    PubMed Central

    Doyle, R J; Nedjat-Haiem, F; Keller, K F; Frasch, C E

    1984-01-01

    The lectin slide agglutination test for Neisseria gonorrhoeae has been modified and improved. Results show that wheat germ agglutinin and soybean lectin agglutinate 100% (193 of 193 tested) of clinical isolates of N. gonorrhoeae. Lectin-reactive meningococci can be readily identified by the hydrolysis of gamma-glutamyl-beta-naphthylamide. Branhamella catarrhalis, Neisseria lactamica, Neisseria sicca, Neisseria subflava, Neisseria perflava, and meningococcal serogroups A, B, C, X, Y, and Z do not interfere with the positive identification of N. gonorrhoeae. The frequently encountered problem of autoagglutination of members of the family Neisseriaceae may be circumvented by a short treatment of cellular suspensions with DNase. Based on agglutination assays, the enzyme treatment did not result in a loss of wheat germ agglutinin receptors from the bacteria. The lectin agglutination test, coupled with the gamma-glutamyl aminopeptidase assay, is proposed as a rapid and accurate means of identifying clinical isolates of gonococci. PMID:6546936

  20. Comparative in vitro activity of the new oral cephalosporin Bay v 3522 against aerobic and anaerobic bacteria.

    PubMed

    Rylander, M; Nord, C E; Norrby, S R

    1990-10-01

    The in vitro activity of the new oral cephalosporin Bay v 3522 against 229 aerobic and 330 anaerobic clinical isolates was determined using the agar dilution technique. For comparison, amoxicillin, amoxicillin/clavulanate, cefaclor, cefadroxil, cefuroxime, cephalexin, ciprofloxacin, clindamycin, co-trimoxazole, doxycycline, erythromycin and metronidazole (only anaerobic bacteria) were tested. Bay v 3522 was found to have high activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pneumoniae, Streptococcus pyogenes, Branhamella catarrhalis, Haemophilus influenzae, anaerobic cocci, Propionibacterium acnes, Clostridium perfringens and fusobacteria. When tested against a higher inoculum or using the broth dilution technique, the activity of Bay v 3522 showed little dependence on inoculum size and the bactericidal activity was similar to inhibitory activity in most bacterial groups. Bay v 3522 may be useful in the treatment of skin, soft tissue and respiratory tract infections. Clinical studies are thus warranted. PMID:2261923

  1. Far from superficial: microbial diversity associated with the skin and mucus of fish

    USGS Publications Warehouse

    Cipriano, Rocco C.; Dove, Alistair

    2011-01-01

    During horizontal or water-borne infection involving an obligate pathogen (e.g. – Aeromonas salmonicida, cause of furunculosis), the pathogen interacted with and influenced the microbial diversity of the dermal mucus of fish. Prior to infection, the prevalent bacterial flora cultured from juvenile Atlantic salmon (Salmo salar) included Pseudomonas fluorescens, Comomonas terrigenia, Acinetobacter sp., Moraxella sp., Pseudomonas dimunita, Alcaligenes denitrificans, Pseudomonas pseudoalcaligenes, and Pseudomonas alcaligenes, Serratia liquefaciens, Aeromonas hydrophila, other motile Aeromonas spp., and Corynebacterium aquaticum. After A. salmonicida was initially detected in this population as an external mucus infection, Acinetobacter sp., Moraxella sp., C. terrigenia, P. fluorescens, and P. dimunita, Staphylococcus sp., and A. hydrophila, were also present in appreciable numbers. Within several weeks, however, the A. salmonicida infection amplified and composed 78% of the total flora in the mucus. Only P. dimunita (4%). P. fluorescens (2%), and C. terrigenia (1%) were cultured at that time and more than a third of these fish showed evidence of a systemic A. salmonicida infection within their kidneys. Eight weeks after oral oxytetracycline treatments, A. salmonicida was no longer isolated from the mucus or kidneys of any fish and glucose inert or other oxidative microbes (e.g., P. fluorescens, C. terrigenia, Acinetobacter sp., Moraxella sp.) were beginning to repopulate the external surface of the salmon in increasing frequency. Still present and composing fairly large percentages of the total flora were A. hydrophila, as well as Enterobacter sp., and P. putrefaciens. A normal microbial diversity was re-established as the fish recovered. In another investigation, reduced biological diversity was noted in the dermal mucus among smallmouth bass that were sampled from the Jackson River (Covington, VA). In these fish, A. hydrophila and P. putrefaciens were the two predominant microorganisms composing 49.5% and 31.2% of the total bacterial flora, despite the absence of systemic infection or any other clinical signs of disease. In another instance, P. fluorescens was the sole bacterium associated with the surface of Atlantic salmon eggs regardless of their viability at the eyed stage of development. Collectively, these results indicate that the kinetics and distributions of the surface bacterial flora on aquatic organisms is affected by numerous factors including pathogen invasion, environmental conditions, and fish culture practices.

  2. Que hay de nuevo acerca de la inmersion? Un Breve Recorrido por sus Fundamentos y por las Investigaciones Actuales (What's New in Immersion? A Brief Overview of Its Bases and Present-Day Research).

    ERIC Educational Resources Information Center

    Sierra, Josu

    This discussion briefly surveys the development of the immersion method of second language teaching, outlining the central program strategies and characteristics using Curtain's and Pesola's descriptions. An overview of recent research focuses on error correction and feedback studies as an example of pedagogical-methodological evolution in this…

  3. Luchando Uniods por la Libertad: Democracy and the Latino/Latina Struggle for Education in the United States 

    E-print Network

    Olivares-Alvarado, Ana Margarita

    2015-04-30

    education and the political sphere from Plato’s Republic to the most recent institutional phenomenon of educational policy in the United States. By tracing the changing aim of education and the specific group of students who are to receive such education... with Classical Greek philosophers Plato and Aristotle clearly reveals the influence their political philosophies have had on the relationship between the educational and sociopolitical spheres throughout the Western tradition. As will be discussed, Plato...

  4. Reconnaissance evaluation of Honduran geothermal sites. Una evaluacion por medio de reconocimiento de seis areas geotermicas en Honduras

    SciTech Connect

    Eppler, D.; Fakundiny, R.; Ritchie, A.

    1986-12-01

    Six geothermal spring sites were selected on the basis of preliminary investigations conducted in Honduras over the last decade and were evaluated in terms of their development potential. Of the six, the Platanares and San Ignacio sites have high base temperatures and high surface fluid discharge rates and appear to have the best potential for further development as sources of electrical power. A third site, Azacualpa, has a high enough base temperature and discharge rate to be considered as a back-up, but the logistical problems involved in geophysical surveys make it less attractive than the two primary sites. Of the remaining three sites, Pavana may be a source of direct-use heat for local agricultural processing. Sambo Creek and El Olivar have either severe logistical problems that would impede further investigation and development or base temperatures and flow rates that are too low to warrant detailed investigation at this time.

  5. TECNOLOGÍAS DE INFORMACIÓN Y COMUNICACIÓN PARA LA PREVENCIÓN Y CONTROL DE LA INFECCIÓN POR EL VIH Y OTRAS ITS*

    PubMed Central

    Curioso, Walter H.; Blas, Magaly M.; Kurth, Ann E.; Klausner, Jeffrey D.

    2010-01-01

    Avances tecnológicos innovadores como Internet, computadoras personales de bolsillo, teléfonos celulares y otros equipos son un arsenal en crecimiento en el esfuerzo de impedir y controlar el VIH y otras infecciones de transmisión sexual (ITS). A pesar que existe una diversidad de tecnologías de información y comunicación en diferentes etapas de desarrollo para la prevención del VIH e ITS, la investigación en esta área se encuentra aún en crecimiento, y el impacto en la incidencia de enfermedad, las evaluaciones con diseños rigurosos y los estudios económicos todavía son muy limitados. Sin embargo, algunas de estas evidencias son prometedoras y poseen un gran potencial para su uso en nuestro medio. En este artículo hemos realizado una revisión sistemática de la literatura relacionada con el uso de la tecnología aplicada a la prevención y control del VIH e ITS. De ser usada apropiadamente, esta tecnología podría mejorar la vigilancia del VIH y otras ITS, diagnóstico, notificación de parejas, prevención, manejo clínico, y capacitación de profesionales de la salud. PMID:26339254

  6. A Rodaviva das Tecnologias Novas tecnologias do origem, em geral, a novos modelos de negcio que por sua vez

    E-print Network

    de Figueiredo, Luiz Henrique

    tecnológica está em curso e recebeu o nome de ASP (Application Service Provider). Nesse modelo o aplicativo. Um marco definitivo para o reflexo dessa reencarnação na economia será a arquitetura .NET da

  7. En otras palabras: Perfeccionamiento del espanol por medio de la traduccion (In Other Words: Perfecting Spanish Language Skills through Translation).

    ERIC Educational Resources Information Center

    Lunn, Patricia V.; Lunsford, Ernest J.

    This publication, written primarily in Spanish, is an activity book designed to teach Spanish through translation based on the theory that, in order to produce an acceptable translation, students must focus their attention on lexical and grammatical detail. The book combines incisive grammar explanations, relevant lexical information, and a wide…

  8. Por favor visite newark.rutgers.edu/strategic-plan-implementation para ms informacin sobre el proceso de plan estratgico.

    E-print Network

    Neimark, Alexander V.

    las capacidades y talentos de los empleados para la misión general de nuestra institución". Para- equipos, enfocados en las siguientes áreas: gestión de talento para empleados, desarrollo profesional reportes de los evaluadores externos: todos contribuirán en deliberaciones futuras. Gestión de Talento para

  9. Como ayudar a los padres a prevenir el envenenamiento por plomo (Helping Parents Prevent Lead Poisoning). ERIC Digest.

    ERIC Educational Resources Information Center

    Binns, Helen J.; Ricks, Omar Benton

    Children are at greater risk than adults for lead poisoning because children absorb lead more readily than adults, and a small amount of lead in children's bodies can do a great deal of harm. This Spanish-language Digest summarizes some of the causes and effects of childhood lead poisoning and suggests some lead poisoning prevention strategies…

  10. "Hace falta ms medios, ms formacin y mayor coordinacin" LA UNIVERSIDAD ESPAOLA APUESTA POR EL INGLS PERO

    E-print Network

    a alumnos y profesores con los medios necesarios para poder defenderse en otro idioma. Se necesita que los "Hace falta más medios, más formación y mayor coordinación" LA UNIVERSIDAD ESPAÑOLA medio centenar de expertos educativos de diferentes ámbitos, se han expuesto los últimos datos de una

  11. El proyecto #changemarketers est impulsado por un grupo de comunicadores, gestores culturales, artistas, publicitarios y contrapublicitarios que creen que

    E-print Network

    Escolano, Francisco

    poder que tenemos para construir y modificar las cosas. O cambiamos o nos cambian. Ha llegado el momento especializado en temas de publicidad para TVE, publica también artículos en medios digitales y tradicionales

  12. A FSICA ESTADSTICA SE ENCARGA DE ESTUDIAR EL COMPORTAMIENTO microscpico de los sistemas compuestos por muchos cuerpos,

    E-print Network

    Méndez Alcaraz, José Miguel

    47 L A FÍSICA ESTADÍSTICA SE ENCARGA DE ESTUDIAR EL COMPORTAMIENTO microscópico de los sistemas macroscópicas de dichos sistemas. En este artículo se presenta una revisión somera del desarrollo histórico de físicas susceptibles de ser medidas, como aceleración y masa. Cuando se tienen muchas partículas, sin

  13. Efectos de Campos Magnéticos en las Tasas de Consumo de Madera por Coptotermes formosanus, la Termita Subterránea de Formosa.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Sixty groups of 500 workers and 50 soldiers of Coptotermes formosanus were maintained in costume designed containers and fed with a piece of red oak wood (Quercus rubra). Twenty of these groups were exposed to permanent magnets with a flux of 800 G. Another 20 groups were exposed to a permanent mag...

  14. enero-marzo2007Cinvestav6 El pelo fue tomado de Beethoven por mi pap, el Dr.

    E-print Network

    inflamación intestinal que, aunque controlada, a menudo lo llevaba a sufrir de cólicos y dolor intestinal, John O´Shea especula sobre la enfermedad de Beethoven señalando que pudo haberse tratado de un daño

  15. Tabla I. Descripcin del trabajo realizado por eje estratgico del proyecto Eje Estratgico Mejora docente y adaptacin al EEES

    E-print Network

    Verschure, Paul

    puesta en marcha de los cursos masivos gratuitos en línea (MOOC) y a la formación y capacitación docente. - Se ha incentivado la puesta en marcha de los cursos MOOC, donde destaca el rol de la Fundación TecnoCampus, agregación destacada del CEI en docencia y pionera en este tipo de cursos (a modo de ejemplo, el curso

  16. TENDENCIAS (/HOME/TENDENCIAS) Por Staff Domingo, 26 Enero, 2014 -14:20 TURBINA MS CHICA QUE UN CENTAVO LE

    E-print Network

    Chiao, Jung-Chih

    capaz de cargar un celular. Son micro-molinos de viento (turbinas) que salieron de los cerebritos de J podrían meter cientos de ellos en un celular y generar energía mediante mover el celular con la mano para

  17. Examen Final de Bioestadistica (14-09-2002) Puntuacion. 2'5 puntos por cada problema resuelto correcta y completamente.

    E-print Network

    Quirós, Fernando

    (elegidos al azar) de una ciudad con la siguiente distribuci´on, seg´un el grupo sangu´ineo y el factor Rh independencia del factor Rh del grupo sangu´ineo para esta ciudad (al nivel de significaci´on = 0 05)? #12;: O A B AB Rh+ 357 317 81 39 Rh- 96 82 18 10 Seg´un estos datos, ¿puede aceptarse la hip´otesis de

  18. Isolation and characterization of heavy metals resistant bacteria from Lagos Lagoon.

    PubMed

    Olukoya, D K; Smith, S I; Ilori, M O

    1997-01-01

    A total of 228 bacteria with an ability to resist toxic heavy metals were isolated from 8 selected sites of the Lagos Lagoon. The bacteria isolated were Staphylococcus sp., Bacillus sp., Pseudomonas sp., Streptococcus sp., Moraxella sp., Escherichia coli, Proteus sp., Klebsiella sp. and Salmonella sp. The heavy metals to which resistance was recorded were mercury, lead, zinc, cobalt, copper and chromium. The lagoon sites from which the highest number of resistant bacteria were isolated were Marina and Ebute-Ero. The heavy metal to which most bacteria were resistant was cobalt, while the least was chromium. The significance of the result is discussed in relation to the Nigerian environment and human health. PMID:9438345

  19. Communicating with Mexican Americans: Por Su Buena Salud = Communicando Con Mexico Americanos: For Their Good Health. Proceedings of the Conference (Houston, TX, September 13-14, 1979).

    ERIC Educational Resources Information Center

    Moore, Thomas J., Ed.; And Others

    The conference focused on the role of the Mexican American's cultural language, tradition, life style, health practices, and media utilization in the design of effective health education and information programs. Representing various local, state, and national health, education, and media organizations, the 108 participants attended sessions on…

  20. A Roda-viva das Tecnologias Novas tecnologias do origem, em geral, a novos modelos de negcio que por sua vez

    E-print Network

    de Figueiredo, Luiz Henrique

    tecnológica está em curso e recebeu o nome de ASP (Application Service Provider). Nesse modelo o aplicativo. Um marco definitivo para o reflexo dessa reencarnação na economia será a arquitetura .NET da

  1. Circular emitida por el Servicio de Prevencin de la Universidad de Alicante y remitida a los siguientes Departamentos e Institutos el 09/06/2015

    E-print Network

    Escolano, Francisco

    material a major grandària de partícula, encara que en altres casos no obstant això els efectes poden ser los efectos pueden ser muy diferentes, aspectos estos que en definitiva pueden plantear riesgos altres riscos, com per exemple incendis i explosions. Donades les circumstàncies anteriors, a Espanya

  2. Comparacion de Modelos de Educacion Sexual en El Conocimiento y Cambio de Actitudes en Practicas Sexuales por Alumnos de Nivel Superior en La Region De Caguas, Puerto Rico

    ERIC Educational Resources Information Center

    Juan, Vallejo Ramos L.

    2012-01-01

    In opposition to the Sexual Education Traditional Model (SETM) that is used in the state schools of Puerto Rico, the Health Beliefs Model (HBM) appears. It facilitates a curricular design that improves the ability of the students to respond to the group pressure by means of attitudes that stimulate sexual conducts of smaller risk of propagation of…

  3. Ameiridae Boeck and Argestidae Por revisited, with establishment of Parameiropsidae, a new family of Harpacticoida (Crustacea, Copepoda) from deep-sea sediments

    NASA Astrophysics Data System (ADS)

    Corgosinho, P. H. C.; Martínez Arbizu, P.

    2010-09-01

    Four new species of Parameiropsis are described from Angola and Guinea Basins and the Arctic Laptev Sea. The male of Parameiropsis poseidonicus sp. n. differs from that of P. neptuni sp. n. and P. senckenbergi sp. n. in antennule segmentation, length of the proximal aesthetasc, length of the outermost seta of the antennary endopod, degree of reduction of the mouthparts, relative length of the inner spine of the basis of thoracopod 1, shape of the furca and body length. The female of P. amphitriteae sp. n. differs from previously described females of other species in the smaller exopod and endpod of thoracopod 1, reduced armature of thoracopods 1-6, length of the outer setae of exopods and endopods of thoracopods 2-4, and mandible exopod weakly developed and fused to the basis. Parameiropsis is redefined by the following autapomorphies: presence of aesthetasc on 3rd segment of female antennule; antenna strong, with endopod curved upwardly, and shape of the outermost (strongly ornamented) spine; triangular labrum; elongated corpus mandibularis, gnathobasis very long; basis of mandibular palp unarmed; elongated maxillule, with long and flexible setae on praecoxal arthrite; basis of the maxilla with strongly modified claw. To discuss the phylogenetic position of Parameiropsis, we revaluated the subfamilies of Ameiridae (viz. Ameirinae and Stenocopiinae) and the family Argestidae. Anoplosomella and Malacopsyllus revealed to be not closely related to Ameiridae and are transferred to Argestidae, sharing with other members of this family the morphology of the mandible gnathobasis, armature of maxilla and armature and length of the first segment of the antennule. Argestoides prehensilis does not show any of the characters that we consider autapomorphic for Argestidae. Instead, it shows many characters in common with several Ameiridae species. Parameiropsis does not have any character that could justify its inclusion within Ameiridae or even within Podogennonta. It also cannot be included satisfactorily within Argestidae nor Exanechentera. Therefore, we here propose a new family for Parameiropsis, with unclear relationships within Harpacticoida. After these taxonomic rearrangements, Ameiridae and Argestidae are considered monophyletic based on certain maxilla characters that we consider autapomorphic for each group. A key to the identification of the known species of Parameiropsis is added at the end.

  4. Estudio de manglares en el rea de La Parguera utilizando imgenes de Landsat TM por los pasados 30 aos con la pgina de internet Earth Explorer

    E-print Network

    Gilbes, Fernando

    varias especies y ecosistemas viven y se alimentan. Siendo esta área de tal importancia, tanto para la pueden hacer pronósticos de vida de tales ecosistemas. Para el estudio de los mismos, se utilizó el

  5. “Como pella a las dueñas, tomelo quien podiere”: anacronismo y representación en las adaptaciones cinemáticas del Libro de buen amor dirigidas por Tomás Aznar y Jaime Bayarri

    E-print Network

    Drayson, E. A.

    2015-01-01

    . Allí conoce a Endrina y se enamora de ella. Trotaconventos se apoya en la hipocresía y el engaño para instigar un escenario de violación posible, faltando del manuscrito original y basado en un episodio semejante del Pamphilus latino. Sin embargo, en...

  6. Atlas geographico de la America septentrional y meridional ... Por ... Thomas Lopez pensionista de S.M. en la corte de Paris an?o de 1758 

    E-print Network

    Lo?pez de Vargas Machuca, Toma?s

    1758-01-01

    The economic competition between the Netherlands, France and England is documented in the atlases published in Amsterdam, Paris and London between 1500 and 1800. However, the relationship between mapping and economic processes remains mostly...

  7. Problemas de topologia. (Espacios normales) Hoja 5 1. Probar que la linea de Sorgenfrey Rs es normal, pero Rs Rs no lo es, (por tanto

    E-print Network

    Martín Peinador, Elena

    normal? 9. Se define la linea entrelazada como el conjunto R de los reales, dotado de la topolog siguientes espacios no son metrizables: a) El producto numerable de l´ineas, R , dotado de la topolog´ia de cajas. b) El producto de una cantidad no numerable de l´ineas, Rc dotado de la topolog´ia producto. #12;

  8. PROBLEMAS PARA A AULA PRATICA, SEMANA 14 Exercicio 1. Mostre que a func~ao definida por (u, v) = f(x, y) com

    E-print Network

    Santos, João

    ) = f(x, y) com u = exy + 1 v = exy + y2 possui, numa bola Br(0, 1), uma inversa local de classe C1 . Sendo g(u, v) esta inversa, calcule g2 u no ponto (2, 2). Exerc´icio 2. Mostre que, numa vizinhan¸ca do¸c~oes de x de classe C1 . Calcule dy dx (1) e dz dx (1) Exerc´icio 3. Mostre que o sistema exy - u + log

  9. "Pig in a poke (gato por liebre)": the "mota" (Calophysus macropterus) fishery, molecular evidence of commercialization in Colombia and toxicological analyses.

    PubMed

    Salinas, Cristian; Cubillos, Juan Camilo; Gómez, Rigoberto; Trujillo, Fernando; Caballero, Susana

    2014-06-01

    Overfishing has affected the population abundance trends of many commercial fish species. In the Amazon, the fishery of a catfish commonly known as "mota" or "piracatinga" (Calophysus macropterus) has become an important economic activity in the region as this species has replaced a number of other overexploited great catfish species in the markets. Due to this high exploitation, ways in which to increase captures have been identified. One strategy is to use decomposing animal carcasses as bait. Such strategy has increased the hunting pressure on endangered species such as caimans and river dolphins. We investigated which catfish species are currently commercialized in Colombian fish markets using DNA barcoding, and measured mercury concentration in the tissues of fish molecularly identified as C. macropterus. We collected 86 fish samples in markets of four Colombian cities. Sixty-eight of these were identified molecularly as C.macropterus. The mercury concentration of 29 such samples was analyzed. Samples presented total Hg concentrations higher than the limit for human consumption established by the WHO (0.5 ?g/g). These results are worrisome and suggest that (1) C. macropterus is a widely used fish species for human consumption in Colombia and (2) C. macropterus has high concentrations of total Hg, making its consumption a public health risk. Results presented here suggest that C. macropterus has replaced capaz in most Colombian markets. This fishery threatens wild species of river dolphins and caimans, and is also a public health risk given the high mercury levels we found in a subsample of these fishes. PMID:24419666

  10. (COLOR) -Pub: PERIODICO ND Doc: 07495M Red: 60% Ed: Primera EDICION Cb: 00 Enviado por: Dia: 10/12/2008 -Hora: 23:46

    E-print Network

    Campàs, Otger

    /12/2008 - Hora: 23:46 IDOYA NOAIN NUEVA YORK Harvard se alía con Adrià, la Fundació Alícia y El Bulli EL con El Bulli y el otro abrirá una cooperación del centro de Cambrid- ge (Massachusetts) con la estudiante de Harvard viajará a Catalunya para co- nocer personalmente El Bulli. Se pla- nea crear además un

  11. Library Safari: Tips for Parents of Young Readers and Explorers = De safari por la biblioteca: Consejos para padres de lectores y exploradores jovenes.

    ERIC Educational Resources Information Center

    Clements, Aedin

    Visiting the library is a great way for parents to encourage their child's imagination and learning. It gives parents the opportunity to model good reading behavior and to show their child that they value books and reading. No matter how young the child is, a trip to the library can be an enjoyable outing for parents and their children. Most…

  12. The effects of the porous buffer layer and doping with dysprosium on internal stresses in the GaInP:Dy/por-GaAs/GaAs(100) heterostructures

    SciTech Connect

    Seredin, P. V.; Gordienko, N. N.; Glotov, A. V.; Zhurbina, I. A.; Domashevskaya, E. P.; Arsent'ev, I. N. Shishkov, M. V.

    2009-08-15

    In structures with a porous buffer layer, residual internal stresses caused by a mismatch between the crystal-lattice parameters of the epitaxial GaInP alloy and the GaAs substrate are redistributed to the porous layer that acts as a buffer and is conducive to disappearance of internal stresses. Doping of the epitaxial layer with dysprosium exerts a similar effect on the internal stresses in the film-substrate structure.

  13. MAC 323 Estruturas de Dados -2004 Refer^encia Principal: Este livro esta disponivel na FNAC (por R$ 48,70).

    E-print Network

    Song, Siang Wun

    ) · n discos no pino A, em ordem decrescente de tamanho, de baixo para cima. · Mover todos os discos para o pino destino sujeito a: ­ mover um disco de cada vez. ­ um disco nunca pode ser colocado sobre) then 3: hanoi(n - 1, A, C, B) 4: mover o disco do topo de C para B 5: hanoi(n - 1, C, B, A) 6: end

  14. MXICO, D.F., noviembre 29 (EL UNIVERSAL).-Por su contribucin a la investigacin y a la ciencia, este martes se

    E-print Network

    Coello, Carlos A. Coello

    Fisiología Celular de la UNAM; medio ambiente José Antonio Toledo Antonio, del Instituto Mexicano del Biomédicas; en ciencias básicas, Ricardo Tapia Ibargüengoytia, del Instituto de Fisiología Celular, y en a Jaime Mas Oliva del Instituto de Fisiología Celular de la UNAM. Finalmente el galardón regional que

  15. UFSC -Universidade Federal de Santa Catarina 03/11/2015 -10:54 Relatrio de Bens Patrimoniais por Setor -Inventrio Fsico Pgina: 1 de 22

    E-print Network

    Hanazaki, Natalia

    ENCONTRADO 1 154,67 SCIH/DG/HU - Serviço de Controle de Infecção Hospitalar 56 26.421,93 NÃO INVENTARIADO 56 ENCONTRADO 2 400,60 COFATH/DA/HU - Coordenadoria de Faturamento Hospitalar 31 6.904,27 ENCONTRADO 19 4

  16. Ciclo de estudos em obras raras: as competncias para a gesto de colees especiais, n. 2/ organizado por Alessandro de Oliveira

    E-print Network

    Liu, I-Shih

    Deus. Rio de Janeiro: Universidade Federal do Rio de Janeiro, 2014. 22 p. 1. Obras raras ­ congressos. Ribeiro, Alessandro de Oliveira Ossola (org.). II. Sérgio, Leonisses Manhã (org.). III. Deus, Cássia C. D

  17. Comparacion de modelos de Educacion Sexual en el conocimiento y cambio de actitudes en practicas sexuales por alumnos de nivel superior en la region de Caguas, Puerto Rico

    NASA Astrophysics Data System (ADS)

    Juan, Vallejo Ramos L.

    In opposition to the Sexual Education Traditional Model (SETM) that is used in the state schools of Puerto Rico, the Health Beliefs Model (HBM) appears. It facilitates a curricular design that improves the ability of the students to respond to the group pressure by means of attitudes that stimulate sexual conducts of smaller risk of propagation of the Sexually Transmitted Diseases (STD). In addition, it provides activities to increase the self-esteem, the communication and the decision making. This investigation had the intention to compare the SETM and the HBM in the increase of knowledge and change of attitudes of high risk of propagation of the STD using a validated questionnaire (Agency of the United States for the International-USAID Development), named "Endesa 2007" and, adapted to Puerto Rico by the Dra.Marta Collazo to a sample of students between the 17 and 19 years of 2 state schools of San Lorenzo, as a pretest, and, selected by convenience. Then, a 10 hours training was administered to half of the students using the SETM to STD and condom use lessons. The other half of the students received additional lessons using the HBM. Finally, both groups took the questionnaire again as a posttest. The sample of students, in average, did not reach the knowledge and basic levels of attitudes towards the STD in the pretest. This reflected 2 possible implications on the SETM. In first place, that the way in which the STD is implemented as part of the Sexual Education curriculum is inefficient. Secondly, the possibility that the acquired information or attitudes does not have permanence. Culminated the questionnaire, the HBM increase the knowledge of the STD in 0.41 points (average) over the SETM. There was not a significant difference between both models, in attitudes, implying that both models are equally effective. The findings suggests that the HBM is more effective increasing the knowledge on the STD, but equally effective than the SETM in attitude change for the Puerto Rican youth.

  18. Evaluating approaches to measuring ocular pain in bovine calves with corneal scarification and infectious bovine keratoconjunctivitis-associated corneal ulcerations.

    PubMed

    Dewell, R D; Millman, S T; Gould, S A; Tofflemire, K L; Whitley, R D; Parsons, R L; Rowe, E W; Liu, F; Wang, C; O'Connor, A M

    2014-03-01

    Infectious bovine keratoconjunctivitis (IBK) is a common ocular disease in cattle, associated with a 6.8 to 13.6 kg decrease in weaning weight. Antibiotic therapy is available but it is unclear if pain mitigation as an adjunct therapy would reduce the weight loss associated with IBK. Before assessing the impact of pain mitigation therapies, it is first necessary to validate approaches to qualifying ocular pain. The objective of this study was to evaluate approaches to qualifying ocular pain in bovine calves (Bos taurus) with IBK. Our a priori assumption was that scarification or corneal ulcerations consistent with IBK are painful compared to normal eyes. To quantify this difference in pain, we assessed 4 tools: pressure algometry-mechanical nociceptive threshold (PA-MNT), corneal touch thresholds (CTT) obtained with the use of a Cochet-Bonnet aesthesiometer, and assessment for the presence of blepharospasm and photophobia as metrics for pain. Using a 1-eye randomized controlled challenge trial, 31 calves with healthy eyes were randomly allocated to treatment groups, and then a left or right eye was randomly assigned for corneal scarification and inoculation with Moraxella bovoculi or Moraxella bovis. A repeated measures analysis of variance was used for PA-MNT, with significance set at P < 0.05. A log (base 10) transformation was used to stabilize the variance, and Tukey's t tests were used to test differences between assessment days for each landmark. Calves had statistically significantly lower PA-MNT scores (which indicates more pain) the day after scarification relative to baseline measurements (4 d before scarification). For example, at 1 landmark the median PA-MNT (kg/force) prescarification was 4.82 (95% confidence interval [CI]: 3.92-5.93) and 3.43 (95% CI: 2.79-4.22) postscarification. These data suggest PA-MNT may be a tool for quantifying ocular pain in calves. No differences (P < 0.1) in PA-MNT scores between scarified and not-scarified eyes were detected for any landmark on any day. This result suggests that the pain response occurs over the entire face, not just the affected eye. Corneal ulcerations consistent with IBK were not associated with statistically significant differences in PA-MNT or CTT at eye or calf levels. Not surprisingly, scarified eyes were more likely to exhibit blepharospasm and photophobia compared to healthy eyes. Due to blepharospasm, the use of the Cochet-Bonnet to evaluate corneal sensitivity by CTT was of limited value. PMID:24504043

  19. Upper respiratory tract infections.

    PubMed

    Jain, N; Lodha, R; Kabra, S K

    2001-12-01

    Acute respiratory infections accounts for 20-40% of outpatient and 12-35% of inpatient attendance in a general hospital. Upper respiratory tract infections including nasopharyngitis, pharyngitis, tonsillitis and otitis media constitute 87.5% of the total episodes of respiratory infections. The vast majority of acute upper respiratory tract infections are caused by viruses. Common cold is caused by viruses in most circumstances and does not require antimicrobial agent unless it is complicated by acute otitis media with effusion, tonsillitis, sinusitis, and lower respiratory tract infection. Sinusitis is commonly associated with common cold. Most instances of rhinosinusitis are viral and therefore, resolve spontaneously without antimicrobial therapy. The most common bacterial agents causing sinusitis are S. pneumoniae, H. influenzae, M. catarrhalis, S. aureus and S. pyogenes. Amoxycillin is antibacterial of choice. The alternative drugs are cefaclor or cephalexin. The latter becomes first line if sinusitis is recurrent or chronic. Acute pharyngitis is commonly caused by viruses and does not need antibiotics. About 15% of the episodes may be due to Group A beta hemolytic streptococcus (GABS). Early initiation of antibiotics in pharyngitis due to GABS can prevent complications such as acute rheumatic fever. The drug of choice is penicillin for 10-14 days. The alternative medications include oral cephalosporins (cefaclor, cephalexin), amoxicillin or macrolides. PMID:11838568

  20. [Acute bacterial exacerbation of chronic obstructive pulmonary disease and biofilm].

    PubMed

    Legnani, Delfino

    2009-07-01

    The lower respiratory tract of patients affected by COPD is constantly colonized by pathogenic microrganisms such as H. influenzae, M. catarrhalis and S. pneumoniae. Role of bacterial colonization of big and small airways in patients affected by COPD is still unclear but it is likely to play a role in directly or indirectly maintaining the vicious circle of infection/inflammation. Colonizer pathogens are capable to stimulate mucus production, to alter the ciliary function by inducing dyskinesia and stasis; in addition, they represent a strong stimulus for neutrophils to come in the airways, which release elastase that, in turn, inhibit the mucus-ciliary function. The same pathogens are responsible for epithelial damage and chronic inflammation, by releasing neutrophilic elastase, leading to the damage progression and obstruction. Recent studies have also shown that infection sustained by H. influenzae is not limited to bronchial mucosa, i.e. surface epithelial cells, but that the pathogen is capable to penetrate cells, so spreading the infection in sub-epithelial cellular layers. In addition, the ability to produce biofilm is another possible defence mechanism which allows them to grow and colonise. Such a mechanism could in part explain the lack of response to antimicrobials and contribute to stimulation of parenchymal inflammatory response, the cause of pathological-anatomic damage which occurs in COPD. The impossibility to eradicate chronic infection and bacterial exacerbations of COPD are likely the elements that promt and worsen obstruction, so determining the disease's progression. PMID:19696555

  1. Broad spectrum antibacterial activity of a mixture of isothiocyanates from nasturtium (Tropaeoli majoris herba) and horseradish (Armoraciae rusticanae radix).

    PubMed

    Conrad, A; Biehler, D; Nobis, T; Richter, H; Engels, I; Biehler, K; Frank, U

    2013-02-01

    Isothiocyanates have been reported to exert antimicrobial activity. These compounds are found in a licensed native preparation of nasturtium (Tropaeoli majoris herba) and horseradish (Armoraciae rusticanae radix) which is used for treatment of upper respiratory and urinary tract infections. The aim of our investigation was to assess the antimicrobial activity of a mixture of the contained benzyl-, allyl-, and phenylethyl- isothiocyanates against clinically important bacterial and fungal pathogens including antimicrobial resistant isolates. Susceptibility testing was performed by agar-dilution technique. Isothiocyanates were mixed in proportions identical to the licensed drug. Minimum inhibitory- and minimum bactericidal concentrations were assessed. The Minimum inhibitory concentration90 was defined as the concentration which inhibited 90% of the microbial species tested. H. influenzae, M. catarrhalis, S. marcescens, P. vulgaris, and Candida spp. were found to be highly susceptible, with minimum inhibitory concentration90 -values ranging between ?0.0005% and 0.004% (v/v) of total ITC. Intermediate susceptibilities were observed for S. aureus, S. pyogenes, S. pneumoniae, K. pneumoniae, E. coli and P. aeruginosa, with Minimum inhibitory concentration90 -values ranging between 0.004% and 0.125% (v/v), but with elevated Minimum bactericidal concentrations90-values (2-7 dilution steps above Minimum inhibitory concentration90). Low susceptibilities were determined for viridans streptococci and enterococci. Interestingly, both resistant and non-resistant bacteria were similarly susceptible to the test preparation. PMID:23447075

  2. Study of in vitro antimicrobial and antiproliferative activities of selected Saharan plants.

    PubMed

    Palici, Ionut F; Liktor-Busa, Erika; Zupkó, István; Touzard, Blaise; Chaieb, Mohamed; Urbán, Edit; Hohmann, Judit

    2015-12-01

    The aim of the present study was the evaluation of the antimicrobial and antiproliferative activities of selected Saharan species, which are applied in the traditional medicine but not studied thoroughly from chemical and pharmacological point of view. The studied plants, namely Anthyllis henoniana, Centropodia forskalii, Cornulaca monacantha, Ephedra alata var. alenda, Euphorbia guyoniana, Helianthemum confertum, Henophyton deserti, Moltkiopsis ciliata and Spartidium saharae were collected from remote areas of North Africa, especially from the Tunisian region of Sahara. After drying and applying the appropriate extraction methods, the plant extracts were tested in antimicrobial screening assay, performed on 19 Gram-positive and -negative strains of microbes. The inhibition zones produced by plant extracts were determined by disc-diffusion method. Remarkable antibacterial activities were exhibited by extracts of Ephedra alata var. alenda and Helianthemum confertum against B. subtilis, M. catarrhalis and methicillin-resistant and non-resistant S. aureus. Minimum inhibitory concentrations of these two species were also determined. Antiproliferative effects of the extracts were evaluated against 4 human adherent cell lines (HeLa, A431, A2780 and MCF7). Notable cell growth inhibition was found for extract of Helianthemum confertum and Euphorbia guyoniana. Our results provided data for selection of some plant species for further detailed pharmacological and phytochemical examinations. PMID:26616371

  3. Culture independent survey of the microbiota of the glassy-winged sharpshooter (Homalodisca vitripennis) using 454 pyrosequencing.

    PubMed

    Hail, Daymon; Lauzìere, Isabelle; Dowd, Scot E; Bextine, Blake

    2011-02-01

    The glassy-winged sharpshooter, Homalodisca vitripennis (Germar), is an invasive pest that has spread across the southern and western United States. H. vitripennis is highly polyphagous and voracious, feeding on at least 100 plant species and consuming up to 100 times its weight in xylem fluid daily. The insect is a vector of the phytopathogen Xylella fastidiosa (Wells), which is the causative agent of Pierce's disease in grapevines. To evaluate the microbial flora associated with H. vitripennis, total DNA extracts from hemolymph, alimentary canal excretions, and whole insect bodies were subjected to 16S rDNA pyrosequencing using the bTEFAP methodology and the resulting sequences (370-520 bp in length) were compared with a curated high quality 16S database derived from GenBank http://www.ncbi.nlm.nih.gov. Species from the genera Wolbachia, Delftia (formerly Pseudomonas), Pectobacterium, Moraxella, Serratia, Bacillus, and many others were detected and a comprehensive picture of the microbiome associated with H. vitripennis was established. Some of the bacteria identified in this report are initial discoveries; providing a breadth of knowledge to the microbial flora of this insect pest can serve as a reservoir of information for developing biological control strategies. PMID:22182607

  4. Laboratory scale bioremediation of diesel hydrocarbon in soil by indigenous bacterial consortium.

    PubMed

    Sharma, Anjana; Rehman, Meenal Budholia

    2009-09-01

    In vitro experiment was performed by taking petrol pump soils and diesel in flasks with the micronutrients and macronutrients supplements. Cemented bioreactors having sterilized soil and diesel was used for in vivo analysis of diesel hydrocarbon degradation. There were two sets of experiments, first having three bioreactors (1) inoculated by KI. pneumoniae subsp. aerogenes with soil and diesel; (2) with addition of NH4NO3; and (3) served as control. In second set, one bioreactor was inoculated by bacterial consortium containing Moraxella saccharolytica, Alteromonas putrefaciens, KI. pneumoniae subsp. aerogenes and Pseudomonas fragi along with soil and diesel. The remaining two bioreactors (having NH4NO3 and control) were similar to the first set. The experiments were incubated for 30 days. Ability of bacterial inoculum to degrade diesel was analyzed through GC-MS. Smaller chain compounds were obtained after experimental period of 30 days. Rate of diesel degradation was better with the present bacterial consortium than individual bacteria. Present bacterial consortium can be a better choice for faster and complete remediation of contaminated hydrocarbon soils. PMID:19957891

  5. A Pyrosequencing Investigation of Differences in the Feline Subgingival Microbiota in Health, Gingivitis and Mild Periodontitis

    PubMed Central

    Harris, Stephen; Croft, Julie; O’Flynn, Ciaran; Deusch, Oliver; Colyer, Alison; Allsopp, Judi; Milella, Lisa; Davis, Ian J.

    2015-01-01

    Periodontitis is the most frequently diagnosed health problem in cats yet little is known about the bacterial species important for the disease. The objective of this study was to identify bacterial species associated with health, gingivitis or mild periodontitis (<25% attachment loss) in feline plaque. Knowledge of these species is a first step in understanding the potential for improving oral health of cats via dietary interventions that alter the proportions of influential species. Subgingival plaque samples were collected from 92 cats with healthy gingiva, gingivitis or mild periodontitis. Pyrosequencing of the V1-V3 region of the 16S rDNA from these plaque samples generated more than one million reads and identified a total of 267 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all gingival health categories, particularly in health along with Moraxella and Fusobacteria. The Peptostreptococcaceae were the most abundant family in gingivitis and mild periodontitis. Logistic regression analysis identified species from various genera that were significantly associated with health, gingivitis or mild periodontitis. The species identified were very similar to those observed in canine plaque in the corresponding health and disease states. Such similarities were not observed between cat and human at the bacterial species level but with disease progression similarities did emerge at the phylum level. This suggests that interventions targeted at human pathogenic species will not be effective for use in cats but there is more potential for commonalities in interventions for cats and dogs. PMID:26605793

  6. Chlorine resistance patterns of bacteria from two drinking water distribution systems.

    PubMed Central

    Ridgway, H F; Olson, B H

    1982-01-01

    The relative chlorine sensitivities of bacteria isolated from chlorinated and unchlorinated drinking water distribution systems were compared by two independent methods. One method measured the toxic effect of free chlorine on bacteria, whereas the other measured the effect of combined chlorine. Bacteria from the chlorinated system were more resistant to both the combined and free forms of chlorine than those from the unchlorinated system, suggesting that there may be selection for more chlorine-tolerant microorganisms in chlorinated waters. Bacteria retained on the surfaces of 2.0-microns Nuclepore membrane filters were significantly more resistant to free chlorine compared to the total microbial population recovered on 0.2-micron membrane filters, presumably because aggregated cells or bacteria attached to suspended particulate matter exhibit more resistance than unassociated microorganisms. In accordance with this hypothesis, scanning electron microscopy of suspended particulate matter from the water samples revealed the presence of attached bacteria. The most resistant microorganisms were able to survive a 2-min exposure to 10 mg of free chlorine per liter. These included gram-positive spore-forming bacilli, actinomycetes, and some micrococci. The most sensitive bacteria were readily killed by chlorine concentrations of 1.0 mg liter-1 or less, and included most gram-positive micrococci, Corynebacterium/Arthrobacter, Klebsiella, Pseudomonas/Alcaligenes, Flavobacterium/Moraxella, and Acinetobacter. Images PMID:7149722

  7. A longitudinal assessment of changes in bacterial community composition associated with the development of periodontal disease in dogs.

    PubMed

    Wallis, Corrin; Marshall, Mark; Colyer, Alison; O'Flynn, Ciaran; Deusch, Oliver; Harris, Stephen

    2015-12-31

    Periodontal disease is the most widespread oral disease in dogs. Whilst the involvement of bacteria in the aetiology of periodontitis is well established the role of individual species and their complex interactions with the host is not well understood. The objective of this research was therefore to perform a longitudinal study in dogs to identify the changes that occur in subgingival bacterial communities during the transition from mild gingivitis to the early stages of periodontitis (<25% attachment loss). Subgingival plaque samples were collected from individual teeth of 52 miniature schnauzer dogs every six weeks for up to 60 weeks. The microbial composition of plaque samples was determined using 454-pyrosequencing of the 16S rDNA. A group of aerobic Gram negative species, including Bergeyella zoohelcum COT-186, Moraxella sp. COT-017, Pasteurellaceae sp. COT-080, and Neisseria shayeganii COT-090 decreased in proportion as teeth progressed to mild periodontitis. In contrast, there was less evidence that increases in the proportion of individual species were associated with the onset of periodontitis, although a number of species (particularly members of the Firmicutes) became more abundant as gingivitis severity increased. There were small increases in Shannon diversity, suggesting that plaque community membership remains relatively stable but that bacterial proportions change during progression into periodontitis. This is the first study to demonstrate the temporal dynamics of the canine oral microbiota; it showed that periodontitis results from a microbial succession predominantly characterised by a reduction of previously abundant, health associated taxa. PMID:26507828

  8. Antibiotic-resistant bacteria in drinking water.

    PubMed Central

    Armstrong, J L; Shigeno, D S; Calomiris, J J; Seidler, R J

    1981-01-01

    We analyzed drinking water from seven communities for multiply antibiotic-resistant (MAR) bacteria (bacteria resistant to two or more antibiotics) and screened the MAR bacterial isolates obtained against five antibiotics by replica plating. Overall, 33.9% of 2,653 standard plate count bacteria from treated drinking waters were MAR. Two different raw water supplies for two communities carried MAR standard plate count bacteria at frequencies of 20.4 and 18.6%, whereas 36.7 and 67.8% of the standard plate count populations from sites within the respective distribution systems were MAR. Isolate identification revealed that MAR gram-positive cocci (Staphylococcus) and MAR gram-negative, nonfermentative rods (Pseudomonas, Alcaligenes, Moraxella-like group M, and Acinetobacter) were more common in drinking waters than in untreated source waters. Site-to-site variations in generic types and differences in the incidences of MAR organisms indicated that shedding of MAR bacteria living in pipelines may have contributed to the MAR populations in tap water. We conclude that the treatment of raw water and its subsequent distribution select for standard plate count bacteria exhibiting the MAR phenotype. PMID:7283426

  9. Predominant bacteria in an activated sludge reactor for the degradation of cutting fluids

    SciTech Connect

    Baker, C.A.; Claus, G.W.; Taylor, P.A.

    1983-01-01

    For the first time, an activated sludge reactor, established for the degradation of cutting fluids, was examined for predominant bacteria. In addition, both total and viable numbers of bacteria in the reactor were determined so that the percentage of each predominant type in the total reactor population could be determined. Three samples were studied, and a total of 15 genera were detected. In each sample, the genus Pseudomonas and the genus Microcyclus were present in high numbers. Three other genera, Acinetobacter, Alcaligenes, and Corynebacterium, were also found in every sample but in lower numbers. In one sample, numerous appendage bacteria were present, and one of these, the genus Seliberia, was the most predominant organism in that sample. However, in the other two samples no appendage bacteria were detected. Six genera were found in this reactor which have not been previously reported in either cutting fluids in use or in other activated sludge systems. These genera were Aeromonas, Hyphomonas, Listeria, Microcyclus, Moraxella, and Spirosoma. None of the predominant bacterial belonged to groups of strict pathogens. 22 references, 6 figures, 3 tables.

  10. A Pyrosequencing Investigation of Differences in the Feline Subgingival Microbiota in Health, Gingivitis and Mild Periodontitis.

    PubMed

    Harris, Stephen; Croft, Julie; O'Flynn, Ciaran; Deusch, Oliver; Colyer, Alison; Allsopp, Judi; Milella, Lisa; Davis, Ian J

    2015-01-01

    Periodontitis is the most frequently diagnosed health problem in cats yet little is known about the bacterial species important for the disease. The objective of this study was to identify bacterial species associated with health, gingivitis or mild periodontitis (<25% attachment loss) in feline plaque. Knowledge of these species is a first step in understanding the potential for improving oral health of cats via dietary interventions that alter the proportions of influential species. Subgingival plaque samples were collected from 92 cats with healthy gingiva, gingivitis or mild periodontitis. Pyrosequencing of the V1-V3 region of the 16S rDNA from these plaque samples generated more than one million reads and identified a total of 267 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all gingival health categories, particularly in health along with Moraxella and Fusobacteria. The Peptostreptococcaceae were the most abundant family in gingivitis and mild periodontitis. Logistic regression analysis identified species from various genera that were significantly associated with health, gingivitis or mild periodontitis. The species identified were very similar to those observed in canine plaque in the corresponding health and disease states. Such similarities were not observed between cat and human at the bacterial species level but with disease progression similarities did emerge at the phylum level. This suggests that interventions targeted at human pathogenic species will not be effective for use in cats but there is more potential for commonalities in interventions for cats and dogs. PMID:26605793

  11. Analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary PCR-restriction fragment length polymorphism fingerprint database for identifying isolates.

    PubMed

    Chen, Yu; Gao, Hongwei; Zhang, Yanming; Deng, Mingjun; Wu, Zhenxing; Zhu, Laihua; Duan, Qing; Xu, Biao; Liang, Chengzhu; Yue, Zhiqin; Xiao, Xizhi

    2012-01-01

    Twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. The bacteria identified were Brevibacillus laterosporus, Leclercia adecarboxylata, Peptococcus niger, Bacillus circulans, Raoultella ornithinolytica, Bacillus subtilis, Bacillus cereus, Bacillus thermobacillus, Bacillus choshinensis, Bacillus sphaericus, Acinetobacter haemolyticus, Sphingomonas paucimobilis, Bacillus thuringiensis, Staphylococcus intermedius, Mycobacteria, Moraxella, Klebsiella pneumoniae, Ralstonia pickettii, Staphylococcus chromogenes, Comamonas testosteroni, and Cupriavidus pauculus. The 16s rDNA gene of each bacterium was amplified using the universal primers 27f and 1492r. The amplicons were digested with AvaI, BamHI, BgII, DraI, EcoRI, EcoRV, HindIII, HinfI, HpaI, PstI, SmaI, TaqII, XbaI, XmaI, AluI, XhoI, and PvuI individually. A specific fingerprint from the PCR-restriction fragment length polymorphism method based on 16s rDNA was obtained for each bacterium. The results showed that the method developed was useful not only for bacterial identification but also for the etiological investigation of pathogens in imported animal hair and wool. PMID:23451394

  12. Characterization of the oral microbiota of healthy cats using next-generation sequencing.

    PubMed

    Sturgeon, A; Pinder, S L; Costa, M C; Weese, J S

    2014-08-01

    The healthy feline oral cavity harbours a rich assemblage of microorganisms, which have not previously been well characterized using modern sequencing technology. The goal of this study was to accurately describe the oral microbiota of 11 healthy cats using next-generation sequencing. Sequencing generated a total of 10,177 operational taxonomic units, representing 273 genera from 18 bacterial phyla. Eight bacterial phyla made up 97.6% of sequences: Proteobacteria (75.2%), Bacteroidetes (9.3%), Firmicutes (6.7%), SR1 (2.7%), Spirochaetes (1.8%), Fusobacteria (1.3%), and Actinobacteria (0.6%). The most prevalent genus-level phylotypes were: an unclassified Pasteurellaceae (18.7%), Moraxella (10.9%), Thermomonas (6.9%), an unclassified Comamonadaceae (5.6%), Neisseria (4.9%), an unclassified Moraxellaceae (4.4%), and Pasteurella (4.3%). Results suggest that the feline oral microbiota are largely conserved between cats at the phylum level, and that the population is highly diverse, rich and even. A strong core microbiome was evident among all cats, yet significant differences in oral bacterial populations were observed across cats in each household. PMID:24680670

  13. Crystal structure of MboIIA methyltransferase.

    SciTech Connect

    Osipiuk, J.; Walsh, M. A.; Joachimiak, A.; Biosciences Division; Univ. of Gdansk; Medical Research Council France

    2003-09-15

    DNA methyltransferases (MTases) are sequence-specific enzymes which transfer a methyl group from S-adenosyl-L-methionine (AdoMet) to the amino group of either cytosine or adenine within a recognized DNA sequence. Methylation of a base in a specific DNA sequence protects DNA from nucleolytic cleavage by restriction enzymes recognizing the same DNA sequence. We have determined at 1.74 {angstrom} resolution the crystal structure of a {beta}-class DNA MTase MboIIA (M {center_dot} MboIIA) from the bacterium Moraxella bovis, the smallest DNA MTase determined to date. M {center_dot} MboIIA methylates the 3' adenine of the pentanucleotide sequence 5'-GAAGA-3'. The protein crystallizes with two molecules in the asymmetric unit which we propose to resemble the dimer when M {center_dot} MboIIA is not bound to DNA. The overall structure of the enzyme closely resembles that of M {center_dot} RsrI. However, the cofactor-binding pocket in M {center_dot} MboIIA forms a closed structure which is in contrast to the open-form structures of other known MTases.

  14. Compositional profile of ?/?-hydrolase fold proteins in mangrove soil metagenomes: prevalence of epoxide hydrolases and haloalkane dehalogenases in oil-contaminated sites

    PubMed Central

    Jiménez, Diego Javier; Dini-Andreote, Francisco; Ottoni, Júlia Ronzella; de Oliveira, Valéria Maia; van Elsas, Jan Dirk; Andreote, Fernando Dini

    2015-01-01

    The occurrence of genes encoding biotechnologically relevant ?/?-hydrolases in mangrove soil microbial communities was assessed using data obtained by whole-metagenome sequencing of four mangroves areas, denoted BrMgv01 to BrMgv04, in São Paulo, Brazil. The sequences (215?Mb in total) were filtered based on local amino acid alignments against the Lipase Engineering Database. In total, 5923 unassembled sequences were affiliated with 30 different ?/?-hydrolase fold superfamilies. The most abundant predicted proteins encompassed cytosolic hydrolases (abH08;???23%), microsomal hydrolases (abH09;???12%) and Moraxella lipase-like proteins (abH04 and abH01;

  15. Diversity of bacterial isolates from commercial and homemade composts.

    PubMed

    Vaz-Moreira, Ivone; Silva, Maria E; Manaia, Célia M; Nunes, Olga C

    2008-05-01

    The diversity of heterotrophic bacterial isolates of three commercial and two homemade composts was studied. The commercial composts were produced from poultry litter (PC), sewage sludge (SC), municipal solid waste (MC), and homemade composts (thermal compost [DC] and vermicompost [VC]) from food wastes. The taxonomic and physiological diversity of the heterotrophic culturable bacteria was assessed using phenotypic and genotypic characterization and the analysis of the partial 16S rRNA gene sequence. Composts DC and SC presented the higher genotypic diversity, as could be inferred from the number of distinct genotypic patterns observed, 28 and 21, respectively. Gram-positive bacteria, mainly Firmicutes, were predominant in all the composts. Some organisms related with taxa rarely reported in composts, as Rhodanobacter spathiphylli, Moraxella osloensis, Lysobacter, Corynebacterium, Pigmentiphaga kullae, and new taxa were also isolated. The highest relative proportion of isolates able to degrade starch was found in compost SC (> 70%), to degrade gelatine in compost DC (> 70%), to degrade Tween 80 in compost PC (> 90%), and to degrade poly-epsilon-caprolactones in compost DC (> 80%). Compost MC presented the lowest relative proportions of isolates able to degrade starch (< 25%), gelatine (< 20%), and poly-epsilon-caprolactone (< 40%). When compared with the others, the homemade composts presented higher relative proportions of Gram-positive isolates able to inhibit the target organisms Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, or Pseudomonas aeruginosa. In compost MC, none of the Gram-positive isolates was able to inhibit those targets. PMID:17876655

  16. INFORME MUJERES INVESTIGADORAS 2014 INFORME MUJERES INVESTIGADORAS. COMISIN ASESORA DE PRESIDENCIA

    E-print Network

    acoso por razón de sexo como por orientación sexual. Actualmente el porcentaje de investigadoras en la DIRECTIVO DEL ORGANISMO ........................................1 DISTRIBUCIÓN DEL PERSONAL POR SEXO DEL PERSONAL CIENTÍFICO POR SEXO ........................2 DISTRIBUCIÓN DEL PERSONAL CIENTÍFICO POR

  17. Inhibitory activity of a standardized elderberry liquid extract against clinically-relevant human respiratory bacterial pathogens and influenza A and B viruses

    PubMed Central

    2011-01-01

    Background Black elderberries (Sambucus nigra L.) are well known as supportive agents against common cold and influenza. It is further known that bacterial super-infection during an influenza virus (IV) infection can lead to severe pneumonia. We have analyzed a standardized elderberry extract (Rubini, BerryPharma AG) for its antimicrobial and antiviral activity using the microtitre broth micro-dilution assay against three Gram-positive bacteria and one Gram-negative bacteria responsible for infections of the upper respiratory tract, as well as cell culture experiments for two different strains of influenza virus. Methods The antimicrobial activity of the elderberry extract was determined by bacterial growth experiments in liquid cultures using the extract at concentrations of 5%, 10%, 15% and 20%. The inhibitory effects were determined by plating the bacteria on agar plates. In addition, the inhibitory potential of the extract on the propagation of human pathogenic H5N1-type influenza A virus isolated from a patient and an influenza B virus strain was investigated using MTT and focus assays. Results For the first time, it was shown that a standardized elderberry liquid extract possesses antimicrobial activity against both Gram-positive bacteria of Streptococcus pyogenes and group C and G Streptococci, and the Gram-negative bacterium Branhamella catarrhalis in liquid cultures. The liquid extract also displays an inhibitory effect on the propagation of human pathogenic influenza viruses. Conclusion Rubini elderberry liquid extract is active against human pathogenic bacteria as well as influenza viruses. The activities shown suggest that additional and alternative approaches to combat infections might be provided by this natural product. PMID:21352539

  18. Non-capsulated and capsulated Haemophilus influenzae in children with acute otitis media in Venezuela: a prospective epidemiological study

    PubMed Central

    2012-01-01

    Background Non-typeable Haemophilus influenzae (NTHi) and Streptococcus pneumoniae are major causes of bacterial acute otitis media (AOM). Data regarding AOM are limited in Latin America. This is the first active surveillance in a private setting in Venezuela to characterize the bacterial etiology of AOM in children < 5 years of age. Methods Between December 2008 and December 2009, 91 AOM episodes (including sporadic, recurrent and treatment failures) were studied in 87 children enrolled into a medical center in Caracas, Venezuela. Middle ear fluid samples were collected either by tympanocentesis or spontaneous otorrhea swab sampling method. Standard laboratory and microbiological techniques were used to identify bacteria and test for antimicrobial resistance. The results were interpreted according to Clinical Laboratory Standards Institute (CLSI) 2009 for non-meningitis isolates. All statistical analyses were performed using SAS 9.1 and Microsoft Excel (for graphical purposes). Results Overall, bacteria were cultured from 69.2% (63 of the 91 episodes); at least one pathogen (S. pneumoniae, H. influenzae, S. pyogenes or M. catarrhalis) was cultured from 65.9% (60/91) of episodes. H. influenzae (55.5%; 35/63 episodes) and S. pneumoniae (34.9%; 22/63 episodes) were the most frequently reported bacteria. Among H. influenzae isolates, 62.9% (22/35 episodes) were non-capsulated (NTHi) and 31.4% (11/35 episodes) were capsulated including types d, a, c and f, across all age groups. Low antibiotic resistance for H. influenzae was observed to amoxicillin/ampicillin (5.7%; 2/35 samples). NTHi was isolated in four of the six H. influenzae positive samples (66.7%) from recurrent episodes. Conclusions We found H. influenzae and S. pneumoniae to be the main pathogens causing AOM in Venezuela. Pneumococcal conjugate vaccines with efficacy against these bacterial pathogens may have the potential to maximize protection against AOM. PMID:22335965

  19. 224. Toda cosa tiene un lado bueno y otro malo: aprende a buscarlos. Toma todo por el lado conveniente. Nunca en forma rabiosa, aunque venga as. Todas las cosas tienen un derecho y

    E-print Network

    Muiño, Ricardo Díez

    conveniente. Nunca en forma rabiosa, aunque venga así. Todas las cosas tienen un derecho y un revés. De modo. En palabras de Gracián, todas las cosas tienen un derecho y un revés y los fracasos temporales en la

  20. Coupling Antibody to CNBr Sepharose 1. Dialyze purified antibody against 0.2 M NaHCO3, pH 8.9. Use SpectraPor 2 (12-14 kDal cut off)

    E-print Network

    Aris, John P.

    times. 2. After dialysis, determine the protein concentration (BioRad Dye method). You will need 2-8 mg antibody solution in 15 ml red cap tube on ice. Save a small amount (~10 µl) of antibody. 3. Wash 10 mls, pH 8.9 and transfer to 1 red-cap 50 ml tube. Rinse all of the Sepharose into the 50 ml tube. Add 0

  1. Angular phase-plate analyzers for measuring the dimensionality of multimode fields J. B. Pors,* A. Aiello, S. S. R. Oemrawsingh, M. P. van Exter, E. R. Eliel, and J. P. Woerdman

    E-print Network

    Exter, Martin van

    profile of a beam. Among these are angular phase plates 4­6 and amplitude holograms 7­9 or phase holograms, we deal with a phase hologram. In recent years, the azimuthal phase dependence of optical fields has,20 , motivated by the advantages that quantum entanglement in a high-dimensional mode space might pro- vide

  2. About to Graduate from High School? Consider Career Education Opportunities. EdSource Student/Parent Guide = Estas por graduarte de la escuela preparatoria? Considera oportunidades para seguir tu educacion de carrera. EdSource guia de estudiantes y padres

    ERIC Educational Resources Information Center

    EdSource, 2006

    2006-01-01

    Getting a sound education is important to a student's ability to make a good living in a field they will enjoy. For many students graduating from high school, that includes high quality career technical (or vocational) education tailored to a specific job. In California, such programs are available in a wide range of fields, from healthcare to the…

  3. POINTSOFIMPACTFACeS ANd PlACeS OF ChANge The CAMPAIgN FOr SyrACuSe uNIverSITy 2008 gIvINg rePOrT

    E-print Network

    McConnell, Terry

    presented Michelangelo: The Man and the Myth, an SU-exclusive exhibition of works by the Renaissance master education in today's economy is not an insignificant challenge. SU has always worked to help students

  4. VISIÓN GENERAL DE LA EVALUACIÓN DEL RIESGO EN SALUD INFANTIL EMPLEANDO UN ENFOQUE POR ETAPAS DE DESARROLLO (American translation is: Overview of a Life Stage Approach to Children's Health Risk Assessment)

    EPA Science Inventory

    Discussing the challenges associated with estimating and interpreting toxicant exposures and health risks from biomonitoring data. This extended abstract was translated in Spanish and published in Acta Toxicologica Argentina.

  5. Fields without Borders: An Anthology of Documentary Writing and Photography by Student Action with Farmworkers' Interns = Campos sin Fronteras: Una Antologia de Obras Escritas y Fotografia por Estudiantes Internos de Accion Estudiantil con Trabajadores Agricolas.

    ERIC Educational Resources Information Center

    Manly, Libby, Ed.; Okie, Alejandra, Ed.; Wiggins, Melinda, Ed.

    In this booklet, essays and poems, presented both in English and in Spanish, portray the feelings, conditions, and economic plight of migrant and seasonal farmworkers in North and South Carolina, often in their own words. A preface describes Student Action with Farmworkers summer internships in which college students spend 10 weeks working with…

  6. DECRETO por el que se expide la Ley General de Transparencia y Acceso a la Informacin Pblica. Al margen un sello con el Escudo Nacional, que dice: Estados Unidos Mexicanos.-Presidencia de la Repblica.

    E-print Network

    información en posesión de cualquier autoridad, entidad, órgano y organismo de los poderes Legislativo, mediante procedimientos sencillos y expeditos; IV. Regular los medios de impugnación y procedimientos para

  7. Viajando por la Carretera de la Educacion Especial: Una Guia para los Padres para Tener un Viaje Feliz y Seguro (Traveling the Special Education Highway: A Parent's Guide to a Safe and Happy Journey).

    ERIC Educational Resources Information Center

    Santa Maria, Karen

    Designed for Spanish-speaking parents, this brochure, written in Spanish, uses a car-trip analogy to describe special education services for students with disabilities. It addresses: (1) child find; (2) initial evaluation and eligibility determination; (3) categories of students who receive special education services and related services; (4)…

  8. NASE (Network for Astronomy School Education) es un grupo de trabajo auspiciado por la Unin Astronmica Internacional (UAI) y su principal objetivo consiste en organizar cursos para la capacitacin

    E-print Network

    Fitze, Patrick

    Unión Astronómica Internacional (UAI) y su principal objetivo consiste en organizar cursos para la capacitación de docentes en didáctica de la astronomía. El propósito de los cursos NASE es promover la observación de fenómenos. Los cursos son teórico-prácticos, con énfasis en el desarrollo de talleres en donde

  9. Guiandose por la Intrincada Senda de la Educacion Especial: Una Guia para Padres y Maestros. Tercera Edicion. (Negotiating the Special Education Maze: A Guide for Parents & Teachers. Third Edition).

    ERIC Educational Resources Information Center

    Anderson, Winifred; Chitwood, Stephen; Hayden, Deidre

    Designed to assist Spanish-speaking parents and teachers in understanding special education procedures, this book describes the process for obtaining school services for children with disabilities. An introduction reviews six major provisions of the Individuals with Disabilities Education Act (IDEA) that relate to children's rights to a free,…

  10. Engaging Foreign Language Learners in a Web 2.0-Mediated Collaborative Learning Process (Inclusión de estudiantes de lenguas extranjeras en procesos colaborativos de aprendizaje mediados por la web 2.0)

    ERIC Educational Resources Information Center

    Cote Parra, Gabriel Eduardo

    2015-01-01

    The purpose of this action research was to explore the types of interactions that foreign language learners experience while using a wiki as a supporting tool for a face-to-face research course. This design allowed me to play a dual role: first, I studied my own classroom setting and students. Second, I implemented a pedagogical intervention based…

  11. Early Canine Plaque Biofilms: Characterization of Key Bacterial Interactions Involved in Initial Colonization of Enamel

    PubMed Central

    Holcombe, Lucy J.; Patel, Niran; Colyer, Alison; Deusch, Oliver; O’Flynn, Ciaran; Harris, Stephen

    2014-01-01

    Periodontal disease (PD) is a significant problem in dogs affecting between 44% and 63.6% of the population. The main etiological agent for PD is plaque, a microbial biofilm that colonizes teeth and causes inflammation of the gingiva. Understanding how this biofilm initiates on the tooth surface is of central importance in developing interventions against PD. Although the stages of plaque development on human teeth have been well characterized little is known about how canine plaque develops. Recent studies of the canine oral microbiome have revealed distinct differences between the canine and human oral environments and the bacterial communities they support, particularly with respect to healthy plaque. These differences mean knowledge about the nature of plaque formation in humans may not be directly translatable to dogs. The aim of this study was to identify the bacterial species important in the early stages of canine plaque formation in vivo and then use isolates of these species in a laboratory biofilm model to develop an understanding of the sequential processes which take place during the initial colonization of enamel. Supra-gingival plaque samples were collected from 12 dogs at 24 and 48 hour time points following a full mouth descale and polish. Pyrosequencing of the 16S rDNA identified 134 operational taxonomic units after statistical analysis. The species with the highest relative abundance were Bergeyella zoohelcum, Neisseria shayeganii and a Moraxella species. Streptococcal species, which tend to dominate early human plaque biofilms, had very low relative abundance. In vitro testing of biofilm formation identified five primary colonizer species, three of which belonged to the genus Neisseria. Using these pioneer bacteria as a starting point, viable two and three species communities were developed. Combining in vivo and in vitro data has led us to construct novel models of how the early canine plaque biofilm develops. PMID:25463050

  12. Early canine plaque biofilms: characterization of key bacterial interactions involved in initial colonization of enamel.

    PubMed

    Holcombe, Lucy J; Patel, Niran; Colyer, Alison; Deusch, Oliver; O'Flynn, Ciaran; Harris, Stephen

    2014-01-01

    Periodontal disease (PD) is a significant problem in dogs affecting between 44% and 63.6% of the population. The main etiological agent for PD is plaque, a microbial biofilm that colonizes teeth and causes inflammation of the gingiva. Understanding how this biofilm initiates on the tooth surface is of central importance in developing interventions against PD. Although the stages of plaque development on human teeth have been well characterized little is known about how canine plaque develops. Recent studies of the canine oral microbiome have revealed distinct differences between the canine and human oral environments and the bacterial communities they support, particularly with respect to healthy plaque. These differences mean knowledge about the nature of plaque formation in humans may not be directly translatable to dogs. The aim of this study was to identify the bacterial species important in the early stages of canine plaque formation in vivo and then use isolates of these species in a laboratory biofilm model to develop an understanding of the sequential processes which take place during the initial colonization of enamel. Supra-gingival plaque samples were collected from 12 dogs at 24 and 48 hour time points following a full mouth descale and polish. Pyrosequencing of the 16S rDNA identified 134 operational taxonomic units after statistical analysis. The species with the highest relative abundance were Bergeyella zoohelcum, Neisseria shayeganii and a Moraxella species. Streptococcal species, which tend to dominate early human plaque biofilms, had very low relative abundance. In vitro testing of biofilm formation identified five primary colonizer species, three of which belonged to the genus Neisseria. Using these pioneer bacteria as a starting point, viable two and three species communities were developed. Combining in vivo and in vitro data has led us to construct novel models of how the early canine plaque biofilm develops. PMID:25463050

  13. A cross-sectional survey of bacterial species in plaque from client owned dogs with healthy gingiva, gingivitis or mild periodontitis.

    PubMed

    Davis, Ian J; Wallis, Corrin; Deusch, Oliver; Colyer, Alison; Milella, Lisa; Loman, Nick; Harris, Stephen

    2013-01-01

    Periodontal disease is the most widespread oral disease in dogs which if left untreated results in significant pain to the pet and loss of dentition. The objective of this study was to identify bacterial species in canine plaque that are significantly associated with health, gingivitis and mild periodontitis (<25% attachment loss). In this survey subgingival plaque samples were collected from 223 dogs with healthy gingiva, gingivitis and mild periodontitis with 72 to 77 samples per health status. DNA was extracted from the plaque samples and subjected to PCR amplification of the V1-V3 region of the 16S rDNA. Pyrosequencing of the PCR amplicons identified a total of 274 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all disease stages, particularly in health along with Moraxella and Bergeyella. Peptostreptococcus, Actinomyces, and Peptostreptococcaceae were the most abundant genera in mild periodontitis. Logistic regression analysis identified species from each of these genera that were significantly associated with health, gingivitis or mild periodontitis. Principal component analysis showed distinct community profiles in health and disease. The species identified show some similarities with health and periodontal disease in humans but also major differences. In contrast to human, healthy canine plaque was found to be dominated by Gram negative bacterial species whereas Gram positive anaerobic species predominate in disease. The scale of this study surpasses previously published research and enhances our understanding of the bacterial species present in canine subgingival plaque and their associations with health and early periodontal disease. PMID:24349448

  14. A Cross-Sectional Survey of Bacterial Species in Plaque from Client Owned Dogs with Healthy Gingiva, Gingivitis or Mild Periodontitis

    PubMed Central

    Davis, Ian J.; Wallis, Corrin; Deusch, Oliver; Colyer, Alison; Milella, Lisa; Loman, Nick; Harris, Stephen

    2013-01-01

    Periodontal disease is the most widespread oral disease in dogs which if left untreated results in significant pain to the pet and loss of dentition. The objective of this study was to identify bacterial species in canine plaque that are significantly associated with health, gingivitis and mild periodontitis (<25% attachment loss). In this survey subgingival plaque samples were collected from 223 dogs with healthy gingiva, gingivitis and mild periodontitis with 72 to 77 samples per health status. DNA was extracted from the plaque samples and subjected to PCR amplification of the V1-V3 region of the 16S rDNA. Pyrosequencing of the PCR amplicons identified a total of 274 operational taxonomic units after bioinformatic and statistical analysis. Porphyromonas was the most abundant genus in all disease stages, particularly in health along with Moraxella and Bergeyella. Peptostreptococcus, Actinomyces, and Peptostreptococcaceae were the most abundant genera in mild periodontitis. Logistic regression analysis identified species from each of these genera that were significantly associated with health, gingivitis or mild periodontitis. Principal component analysis showed distinct community profiles in health and disease. The species identified show some similarities with health and periodontal disease in humans but also major differences. In contrast to human, healthy canine plaque was found to be dominated by Gram negative bacterial species whereas Gram positive anaerobic species predominate in disease. The scale of this study surpasses previously published research and enhances our understanding of the bacterial species present in canine subgingival plaque and their associations with health and early periodontal disease. PMID:24349448

  15. A randomized and blinded field trial to assess the efficacy of an autogenous vaccine to prevent naturally occurring infectious bovine keratoconjunctivis (IBK) in beef calves.

    PubMed

    Funk, L; O'Connor, A M; Maroney, M; Engelken, T; Cooper, V L; Kinyon, J; Plummer, P

    2009-07-23

    A randomized and blinded 2-arm parallel trial was conducted to assess the efficacy of an autogenous vaccine to prevent naturally occurring infectious bovine keratoconjunctivis (IBK) in beef calves. The trial was managed between May and November 2008 on university owned farms in Iowa and Wisconsin. The vaccine at Iowa contained Moraxella bovoculi (M. bovoculi) while the organism used in the Wisconsin herds vaccine was Branhemella ovis (B. ovis renamed M. ovis). Calves born between January and May 2008 without visible corneal lesions were randomized to receive an autogenous vaccine or placebo vaccine using a computer generated sequence. Two subcutaneous doses were administered 21-28 days apart. Allocation to treatment was concealed using bottles marked A or B. Staff were blind to the treatment allocation. The primary outcome was IBK cumulative incidence over the study period. The secondary outcome was weaning weight. Only the Iowa herd met the criteria for an "at-risk" herd i.e. >15% IBK in unvaccinated calves and M. bovoculi isolation from IBK cases. Analysis was "per-protocol". The cumulative incidence of IBK was 47/105 in vaccinated calves and 49/109 in unvaccinated calves (unadjusted odds ratio=0.99, 95% CI: 0.58-1.70). Weight at weaning did not differ between the vaccinated cohort 148kg (SD: +/-27) and unvaccinated cohort 146kg (SD: +/-26) (unadjusted beta=1.5 and 95% CI: -5.5 to 8.6). Results indicate that the autogenous vaccine was ineffective in this study population. PMID:19531391

  16. Different Selective Effects on Rhizosphere Bacteria Exerted by Genetically Modified versus Conventional Potato Lines

    PubMed Central

    Hannula, Silja Emilia; Andreote, Fernando Dini; Pereira e Silva, Michele de Cássia; Salles, Joana Falcão; de Boer, Wietse; van Veen, Johannes; van Elsas, Jan Dirk

    2013-01-01

    Background In this study, we assessed the actively metabolizing bacteria in the rhizosphere of potato using two potato cultivars, i.e. the genetically-modified (GM) cultivar Modena (having tubers with altered starch content) and the near-isogenic non-GM cultivar Karnico. To achieve our aims, we pulse-labelled plants at EC90 stage with 13C-CO2 and analysed their rhizosphere microbial communities 24 h, 5 and 12 days following the pulse. In the analyses, phospholipid fatty acid/stable isotope probing (PLFA-SIP) as well as RNA-SIP followed by reverse transcription and PCR-DGGE and clone library analysis, were used to determine the bacterial groups that actively respond to the root-released 13C labelled carbonaceous compounds. Methodology/Principal findings The PLFA-SIP data revealed major roles of bacteria in the uptake of root-released 13C carbon, which grossly increased with time. Gram-negative bacteria, including members of the genera Pseudomonas and Burkholderia, were strong accumulators of the 13C-labeled compounds at the two cultivars, whereas Gram-positive bacteria were lesser responders. PCR-DGGE analysis of cDNA produced from the two cultivar types showed that these had selected different bacterial, alpha- and betaproteobacterial communities at all time points. Moreover, an effect of time was observed, indicating dynamism in the structure of the active bacterial communities. PCR-DGGE as well as clone library analyses revealed that the main bacterial responders at cultivar Karnico were taxonomically affiliated with the genus Pseudomonas, next to Gluconacetobacter and Paracoccus. Cultivar Modena mainly attracted Burkholderia, next to Moraxella-like (Moraxellaceae family) and Sphingomonas types. Conclusions/Significance Based on the use of Pseudomonas and Burkholderia as proxies for differentially-selected bacterial genera, we conclude that the selective forces exerted by potato cultivar Modena on the active bacterial populations differed from those exerted by cultivar Karnico. PMID:23844136

  17. 0.5setgray00.5setgray1 Determinao de coeficientes em equaes

    E-print Network

    Cipolatti, Rolci

    ~ao tivessem sido previstas por James Clark Maxwell em 1862 e observadas experimentalmente por Heinrich Hertz~ao tivessem sido previstas por James Clark Maxwell em 1862 e observadas experimentalmente por Heinrich Hertz

  18. SECCIN EXTERNA DE TOXICOLOGA Personal acadmico y temas de investigacin

    E-print Network

    , 2000) Universidad de Surrey, Guildord, Surrey, Inglaterra. Temas de investigación: Biología celular y molecular de la respuesta tóxica por estrés oxidativo, muerte celular por apoptosis, señalización redox otros antioxidantes. Inducción de muerte celular por apoptosis inducida por agentes citotóxicos y

  19. 16S rDNA PCR-DGGE and sequencing in the diagnosis of neonatal late-onset septicemia.

    PubMed

    Liu, Dong; Du, Lizhong; Yu, Jialin; Li, Luquan; Ai, Qing; Feng, Jinxing; Song, Chao

    2015-10-01

    The 16S rDNA polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) and sequencing method has been demonstrated to be valuable in detecting pathogens in the blood of patients suffering from fever or neutropenia. However, its use in the diagnosis of neonatal late?onset septicemia (LOS) has not yet been reported. The aim of the present study was to investigate the efficiency of this method in detection of the type of bacterial infection in neonatal LOS. Blood specimens from 60 neonates in whom LOS was suspected were collected. Fourteen culture positive blood samples and 24 spiked 'infected' blood samples were analyzed by the 16S rDNA PCR?DGGE and sequencing method or by pathogen?specific PCR. Only in 5 of the 14 cases did the results of 16S rDNA PCR?DGGE and sequencing match with the results of blood culturing. In the other 9 cases, the blood culture failed to detect bacteria, such as Neisseria sp. and Moraxella sp., which were detected by 16S rDNA PCR?DGGE and sequencing. Furthermore, the 16S rDNA PCR?DGGE and sequencing failed to detect blood culture?proven bacteria, such as Klebsiella pneumonia. A competitive inhibitory effect in 16S rDNA PCR amplification may lead to the discrepancy between pathogen?specific PCR and spiked 'infected' blood samples. When a certain species of bacteria was detected by 16S rDNA PCR, the competitive inhibitory effect presented a higher sensitivity in detecting this species in the blood samples that contained bacterial DNA only from this species compared with the blood samples that were blended with other bacterial DNAs. In conclusion, 16S rDNA PCR?DGGE and sequencing can detect a more comprehensive spectrum of pathogens than blood culture. However, the competitive inhibitory effect, which may lead to false negative results should be taken into consideration when the 16S rDNA PCR-DGGE and sequencing method is applied to the diagnosis of neonatal LOS. PMID:26238862

  20. Fatiga (PDQ)—Versión para profesionales de salud

    Cancer.gov

    Resumen de información revisada por expertos acerca de la fatiga, una afección caracterizada por extremo cansancio e incapacidad para funcionar por la falta de energía, que a menudo se observa como una complicación del cáncer y su tratamiento.

  1. Terrorismo ayer y hoy: El desatino de la Fraccin del Ejrcito Rojo

    E-print Network

    Escolano, Francisco

    complejos como por ejemplo el famoso PRISM, desvelado recientemente por el exempleado de la CIA, Edward Snowden y utilizado actualmente por la Agencia de Seguridad Nacional estadounidense (NSA) para la

  2. UNIVERSIDAD DE SANTIAGO DE CHILE FACULTAD DE CIENCIAS

    E-print Network

    amiga "Cote", por convertirse en una de mis primeras amigas cuando comenzamos esta etapa universitaria amiga "Clau", que escucha y me alienta, cuando no he pasado por buenos momentos. Y por último (porque

  3. CENTRO CUANTA ( ) N DE PROYECTOS CIENCIAS 11.745.243,02 115

    E-print Network

    Autonoma de Madrid, Universidad

    . Proyectos gestionados por el Servicio de Investigación de la UAM (año 2007): distribución por sexo del.082.544,02 555 123 Fondos de investigación art. 83 LOU, concedidos en el año 2007, distribuidos por centro y sexo

  4. Fatiga (PDQ)—Versión para pacientes

    Cancer.gov

    Resumen de información revisada por expertos acerca de la fatiga, una afección caracterizada por extremo cansancio e incapacidad para funcionar por la falta de energía, que a menudo se observa como una complicación del cáncer y su tratamiento.

  5. UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL INSTITUTO DE MATEMATICA

    E-print Network

    Mendes, Luis Gustavo Doninelli

    ¸co a Deus por uma oportunidade deste tamanho. Pela fam´ilia acolhedora, pela esposa carinhosa e pelos amigos companheiros. Agrade¸co a Deus por me dar uma vida com tantas alegrias e conquistas. Espero um dia fazer por

  6. CCOONNSSEEJJOO SSUUPPEERRIIOORR DDEE IINNVVEESSTTIIGGAACCIIOONNEESS

    E-print Network

    Fitze, Patrick

    SEXO..........................................................................................3 DISTRIBUCIÓN DEL PERSONAL POR SEXO / RELACIÓN LABORAL.................................................3 DISTRIBUCIÓN DEL PERSONAL FUNCIONARIO POR SEXO/CUERPO-ESCALA.........................3 DISTRIBUCIÓN DEL

  7. INFORME MUJERES INVESTIGADORAS 2011 http://www.csic.es/web/guest/mujeres-y-ciencia

    E-print Network

    Fitze, Patrick

    ........................................3 DISTRIBUCIÓN DEL PERSONAL POR SEXO/RELACIÓN LABORAL .........3 GRÁFICA "TIJERA" PERSONAL INVESTIGADOR .................................3 EDAD MEDIA DEL PERSONAL CIENTÍFICO POR SEXO

  8. Sermonario en lengua mexicana donde se contiene (por el orden del Misal nuevo Romano) dos sermones en todas las Dominicas y Festividades principales de todo el año ; y otro en las Fiestas de los Santos : con sus vidas, y Comunes. Con un Catecismo 

    E-print Network

    Juan de la Anunciació n, fray, 1514-1594 Sermonario en lengua mexicana donde se contiene (por el orden del Misal nuevo Romano) dos sermones en todas las Dominicas y Festividades principales de todo el añ o; y otro en las Fiestas de los Santos : con sus vidas, y Comunes. Con un Catecismo  Juan de la Anunciació n, fray, 1514-1594 Safer Food with Irradiation (Spanish)  Thompson, Britta; Vestal, Andy; Van Laanen, Peggy

    2003-01-21

    . Muchosestudios cient?ficos han confirmado que los ali-mentos irradiados no son t?xicos y no causanproblemas de salud . Los astronautas han ingeri- do alimentos irradiados desde el inicio del pro-grama espacial sin ninguna consecuencia malapara la salud . ?La...:H7, la Salmonela , la C ampylobacteria , la Listeria y otras bacterias da?inas que pueden estar en losalimentos crudos . La irradiaci?n aumenta la vida de anaquel (alma-cenamiento) y retrasa el proceso de maduraci?nde algunos alimentos frescos...

  9. Los Hispanos: Problemas y Oportunidades. Resumen de la Actual Situacion Demografica, Economica, Social y Politica de los Hispanos en los Estados Unidos y de las Iniciativas Tomadas por la Fundacion Ford Para Hacer Frente a las Necesidades de esta Poblacion en Aumento y Determinar sus Efectos Sobre la Sociedad Estadounidense. Documento de Trabajo de la Fundacion Ford, No. 436.

    ERIC Educational Resources Information Center

    Ford Foundation, New York, NY.

    The Hispanic population's growing impact on American society has caused the Ford Foundation to explore new Foundation initiatives. The 1980 census revealed 14.6 million Hispanics: 60% Mexican American; 14% Puerto Rican; 6% Cuban, and 20% Other. The Hispanic population in the United States is growing and is characterized by diversity; rapid growth…

  10. Exploring with Technology: Activities for Families with Children Ages 3 to 5 [Presented by]"Between the Lions[R]" = Exploremos con tecnologia: Actividades para familias con ninos de 3 a 5 anos [presentado por]"Between the Lions[R]."

    ERIC Educational Resources Information Center

    WGBH-TV, Boston, MA.

    "Between the Lions" is a Public Broadcasting System program promoting literacy for children ages 4 through 7 years combining state-of-the-art puppetry, animation, live action, and music to achieve its mission of helping young children learn to read. This guide, in English- and Spanish-language versions, provides computer literacy activities for…

  11. Words All around Us: Activities for Families with Children Ages 3 to 5 [Presented by]"Between the Lions[R]" = Palabras y palabras a nuestro alrededor: Actividades para familias con ninos de 3 a 5 anos [presentado por]"Between the Lions[R]."

    ERIC Educational Resources Information Center

    WGBH-TV, Boston, MA.

    "Between the Lions" is a Public Broadcasting System program promoting literacy for children ages 4 through 7 years combining state-of-the-art puppetry, animation, live action, and music to achieve its mission of helping young children learn to read. This guide, in English- and Spanish-language versions, provides literacy activities for parents and…

  12. Talking, Singing, Rhyming: Activities for Families with Children Ages 3 to 5 [Presented by]"Between the Lions[R]" = Hablar, cantar, recitar: Actividades para familias con ninos de 3 a 5 anos [presentado por]"Between the Lions[R]."

    ERIC Educational Resources Information Center

    WGBH-TV, Boston, MA.

    "Between the Lions" is a Public Broadcasting System program promoting literacy for children ages 4 through 7 years combining state-of-the-art puppetry, animation, live action, and music to achieve its mission of helping young children learn to read. This guide, in English- and Spanish-language versions, provides literacy activities for parents and…

  13. The Impact of Regional Differences on Elementary School Teachers' Attitudes towards Their Students' Use of Code Switching in a South Texas School District (El impacto de las diferencias regionales en las actitudes de docentes de primaria respecto a la alternancia de códigos por parte de los estudiantes en un distrito escolar del sur de Texas)

    ERIC Educational Resources Information Center

    Nava Gómez, Guadalupe Nancy; García, Hilda

    2012-01-01

    This study focused on investigating whether the teachers' geographical distribution influences their attitudes towards their students' use of code switching. The study was guided by the following research question: Are there differences between teachers' opinions of the north elementary schools and teachers' opinions of the…

  14. Palabras del Secretario de Educacion Publica en la reunion anual de directores de education federal e inspectores generales en los estados que se rigen por el calendario "A". (Address by the Minister of Education at the Annual Meeting of Directors of Federal Education and Inspectors General in Calendar "A" States).

    ERIC Educational Resources Information Center

    Yanez, Agustin

    This document is an English-language abstract (approximately 1,500 words) of a speech by the Mexican Minister of Education at an annual educators meeting. The Minister dealt with the administration and quality of education, the role of the directors and the duties towards them of the inspectors, and the main features of the reform of national…

  15. Lo que los educadores necesitan saber sobre...El agrupamiento por habilidad [y] La compactacion del curriculum [y] Los alumnos dotados y el aprendizaje cooperativo [y] La actividad tutoral. Guias practica (What Educators Need To Know about...Ability Grouping [and] Curriculum Compacting [and] Gifted Students and Cooperative Learning [and] Mentoring. Practitioners' Guides).

    ERIC Educational Resources Information Center

    Siegle, Del, Ed.

    These four pamphlets in Spanish offer guidelines supported by theory-driven quality research that is problem-based, practice-relevant, and consumer-oriented. Each pamphlet has a section summarizing research from the literature or topic notes as well as implications for the classroom. The first guide offers principles for teachers concerning the…

  16. Laser fusion and glass forming of high iron bearing SiO2-CaO-Fe2O3 batches (Fusin y vitrificacin por lser de vidrios SiO2-CaO-Fe2O3 con altas cantidades de hierro)

    E-print Network

    Vásquez, Carlos

    Laser fusion and glass forming of high iron bearing SiO2-CaO-Fe2O3 batches (Fusión y vitrificación bearing glasses for biomedical applications. The high amount of Fe2O3 (40-80%), which is an intermediary (instead of a glass forming) oxide, implies a difficult formation of glass. Therefore a rapid cooling

  17. A Visit to the Library: Activities for Families with Children Ages 3 to 5 [Presented by]"Between the Lions[R]" = Una visita a la biblioteca: Actividades para familias con ninos de 3 a 5 anos [presentado por]"Between the Lions[R]."

    ERIC Educational Resources Information Center

    WGBH-TV, Boston, MA.

    "Between the Lions" is a Public Broadcasting System program promoting literacy for children ages 4 through 7 years combining state-of-the-art puppetry, animation, live action, and music to achieve its mission of helping young children learn to read. This guide, in English- and Spanish-language versions, provides suggestions for parents of 3- to…

  18. Art and Writing: Activities for Families with Children Ages 3 to 5 [Presented by]"Between the Lions[R]" = Arte y escritura: Actividades para familias con ninos de 3 a 5 anos [presentado por]"Between the Lions[R]."

    ERIC Educational Resources Information Center

    WGBH-TV, Boston, MA.

    "Between the Lions" is a Public Broadcasting System program promoting literacy for children ages 4 through 7 years combining state-of-the-art puppetry, animation, live action, and music to achieve its mission of helping young children learn to read. This guide, in English- and Spanish-language versions, provides literacy activities for parents and…

  19. For a Child, Life is a Creative Adventure: Supporting Development and Learning through Art, Music, Movement, and Dialogue. A Guide for Parents and Professionals. = Para los ninos, la vida es una aventura creativa: Como estimular el desarrollo y el aprendizaje por medio de las artes visuales, la musica, el movimiento y el dialogo. Guia para padres de familia y profesionales.

    ERIC Educational Resources Information Center

    Cohen, Elena

    Recognizing that creativity facilitates children's learning and development, the Head Start Program Performance Standards require Head Start programs to include opportunities for creative self-expression. This guide with accompanying videotape, both in English- and Spanish- language versions, encourages and assists adults to support children's…

  20. A protein secretion system linked to bacteroidete gliding motility and pathogenesis

    PubMed Central

    Sato, Keiko; Naito, Mariko; Yukitake, Hideharu; Hirakawa, Hideki; Shoji, Mikio; McBride, Mark J.; Rhodes, Ryan G.; Nakayama, Koji

    2009-01-01

    Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Protein secretion systems common to other Gram-negative bacteria are lacking in P. gingivalis, but several proteins, including PorT, have been linked to gingipain secretion. Comparative genome analysis and genetic experiments revealed 11 additional proteins involved in gingipain secretion. Six of these (PorK, PorL, PorM, PorN, PorW, and Sov) were similar in sequence to Flavobacterium johnsoniae gliding motility proteins, and two others (PorX and PorY) were putative two-component system regulatory proteins. Real-time RT-PCR analysis revealed that porK, porL, porM, porN, porP, porT, and sov were down-regulated in P. gingivalis porX and porY mutants. Disruption of the F. johnsoniae porT ortholog resulted in defects in motility, chitinase secretion, and translocation of a gliding motility protein, SprB adhesin, to the cell surface, providing a link between a unique protein translocation system and a motility apparatus in members of the Bacteroidetes phylum. PMID:19966289

  1. A protein secretion system linked to bacteroidete gliding motility and pathogenesis.

    PubMed

    Sato, Keiko; Naito, Mariko; Yukitake, Hideharu; Hirakawa, Hideki; Shoji, Mikio; McBride, Mark J; Rhodes, Ryan G; Nakayama, Koji

    2010-01-01

    Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Protein secretion systems common to other Gram-negative bacteria are lacking in P. gingivalis, but several proteins, including PorT, have been linked to gingipain secretion. Comparative genome analysis and genetic experiments revealed 11 additional proteins involved in gingipain secretion. Six of these (PorK, PorL, PorM, PorN, PorW, and Sov) were similar in sequence to Flavobacterium johnsoniae gliding motility proteins, and two others (PorX and PorY) were putative two-component system regulatory proteins. Real-time RT-PCR analysis revealed that porK, porL, porM, porN, porP, porT, and sov were down-regulated in P. gingivalis porX and porY mutants. Disruption of the F. johnsoniae porT ortholog resulted in defects in motility, chitinase secretion, and translocation of a gliding motility protein, SprB adhesin, to the cell surface, providing a link between a unique protein translocation system and a motility apparatus in members of the Bacteroidetes phylum. PMID:19966289

  2. Uma Ferramenta para a Composio de "Vdeos Interativos"

    E-print Network

    Barbosa, Alberto

    a Internet/Web, a multimídia, os jogos por computador, e outras tecnologias que em poucos anos passaram por, sem uma representação adequada de aspectos da narrativa. Ao contrário disso, nos jogos, por exemplo, exatamente como fazem os jogos. Portanto, os problemas de interatividade e engajamento por si só també

  3. CCOONNSSEEJJOO SSUUPPEERRIIOORR DDEE IINNVVEESSTTIIGGAACCIIOONNEESS CCIIEENNTTFFIICCAASS

    E-print Network

    Fitze, Patrick

    NIVEL 30 3 DISTRIBUCIÓN DEL PERSONAL POR SEXO/RELACIÓN LABORAL 4 DISTRIBUCIÓN DEL PERSONAL FUNCIONARIO POR SEXO/CUERPO-ESCALA 4 DISTRIBUCIÓN DEL PERSONAL CIENTÍFICO POR ÁREAS DE INVESTIGACIÓN 5 PERSONAL POR SEXO/RELACIÓN LABORAL HOMBRES MUJERES PERSONAL FUNCIONARIO 2.618 2.084 PERSONAL LABORAL FIJO

  4. RELATRIO DE AUDITORIA ANUAL DE CONTAS N 224760 -2 PARTE

    E-print Network

    Maier, Rudolf Richard

    do CPGF, quando houver, bem como a relevância dos objetos adquiridos por meio desta modalidade. Essas processo e que este seja acompanhado da relação de gastos efetuados por meio de saques e por meio de CGPS que essas medidas sejam implementadas, a FUB deverá #12;estabelecê-las por meio de seus normativos

  5. SECCIN EXTERNA DE TOXICOLOGA Personal acadmico y temas de investigacin

    E-print Network

    celulares de ori- gen murino, humano, aislados celulares obtenidos a partir de individuos expues- tos y celular y molecular de la respuesta tóxica por estrés oxidativo, muerte celular por apoptosis, regulación de GSH y otros antioxidantes. Inducción de muerte celular por apoptosis inducida por agentes

  6. SEGURO DE ACCIDENTES Y RESPONSABILIDAD CIVIL PARA ALUMNOS DE LA UNIVERSIDAD DE MURCIA

    E-print Network

    Acacio, Manuel

    100.000 *Desplazamiento de un familiar por hospitalización superior a 5 días en el extranjero Hasta 1.500 grado, para alumnos desplazados en el extranjero Hasta 1.500 Gastos por pérdida de clases por accidente, más de 90 días, en España 1.500 Infortunio familiar por accidente de circulación del padre o la madre

  7. Ultrafast charge separation and charge stabilization in axially linked 'tetrathiafulvalene-aluminum(iii) porphyrin-gold(iii) porphyrin' reaction center mimics.

    PubMed

    Poddutoori, Prashanth K; Lim, Gary N; Vassiliev, Serguei; D'Souza, Francis

    2015-10-21

    The axial bonding ability of aluminum(iii) porphyrin (AlPor) has been exploited to synthesize the vertically linked dyad 'aluminum(iii) porphyrin-gold(iii) porphyrin' (AlPor-Ph-AuPor(+)) and the two corresponding self-assembled triads 'tetrathiafulvalene-aluminum(iii) porphyrin-gold(iii) porphyrin' (TTF-py?AlPor-Ph-AuPor(+) and TTF-Ph-py?AlPor-Ph-AuPor(+)). The unique topology of these triads provides an excellent opportunity to investigate the sequential electron transfer in the perpendicular direction to the AlPor plane where the AlPor acts as a photosensitizer and primary electron donor while the AuPor and TTF serve as an electron acceptor and donor, respectively. The ground state properties of the dyad and triad suggest that there are no direct intramolecular interactions between the oppositely disposed AuPor and TTF units of the triad. However, the NMR and UV-visible absorption studies of the dyad reveal intermolecular interactions in non-coordinating solvents due to the coordination of counterion PF6(-) to the Al center of AlPor. Steady-state and femtosecond transient absorption studies of the dyad show that the lowest excited singlet state of AlPor ((1)AlPor*) is strongly quenched by ultrafast electron transfer to AuPor(+) with a time constant of 3.16 ps. The resulting charge separated state (AlPor(+)?-AuPor?) decays to ground state biexponentially with time constants of 27.26 and 2557 ps. Analogously, upon photo-excitation the triads also produce the same primary radical pair (AlPor(+)?-AuPor?). However, the formed radical pair is further involved in a rapid hole transfer from AlPor(+)? to TTF to form a stable final radical pair TTF(+)?-AlPor-AuPor?. The lifetime of the charge separated state exhibits an increase from 27.26 ps in AlPor-Ph-AuPor to 1393 ps in TTF-py?AlPor-Ph-AuPor(+) and 1484 ps in TTF-Ph-py?AlPor-Ph-AuPor(+). These results reveal successful charge stabilization in the self-assembled supramolecular reaction center mimics constructed via the axial linkage strategy. PMID:26388225

  8. Reference Guide for Texas Ranchers (Spanish) 

    E-print Network

    McGinty, Allan

    2005-11-16

    Mida el ancho del ?rea rociado (pies) l Calcule seg?n lo siguiente: Galones utilizados x 66 = los galones Ancho del ?rea rociada (pies) rociados por acre Capacidad del tanque (galones) = acres rociados Galones rociados por... por un inyector por el tiempo requerido para recorrer 100 pies. l Calcule seg?n lo siguiente: 40 x onzas rociadas = galones rociados Distancia entre boquillas (pulg) por acre Capacidad del tanque (galones) = acres...

  9. VI Jornada de Algebra Universidade Federal do Parana -Curitiba

    E-print Network

    Paraná, Universidade Federal do

    aspectos geom´etricos por meio de objetos e ferramentas alg´ebricas. Nesta palestra de divulga abordar quest~oes topol´ogicas por meio de semigrupos num´ericos e aspectos anal´iticos por meio de an por meio do semigrupo inverso S(G) introduzido por R. Exel para servir a¸c~oes parciais e representa

  10. Plant Protochlorophyllide Oxidoreductases A and B: CATALYTIC EFFICIENCY AND INITIAL REACTION STEPS.

    PubMed

    Garrone, Alessio; Archipowa, Nataliya; Zipfel, Peter F; Hermann, Gudrun; Dietzek, Benjamin

    2015-11-20

    The enzyme protochlorophyllide oxidoreductase (POR, EC 1.3.1.33) has a key role in plant development. It catalyzes one of the later steps in chlorophyll synthesis, the light-induced reduction of protochlorophyllide (PChlide) into chlorophyllide (Chlide) in the presence of NADPH. Two isozymes of plant POR, POR A and POR B from barley, which differ in their function during plant life, are compared with respect to their substrate binding affinity, catalytic efficiency, and catalytic mechanism. POR B as compared with POR A shows an 5-fold higher binding affinity for PChlide and an about 6-fold higher catalytic efficiency measured as kcat/Km. Based on the reaction intermediates, which can be trapped at low temperatures the same reaction mechanism operates in both POR A and POR B. In contrast to results reported for POR enzymes from cyanobacteria, the initial light-driven step, which occurs at temperatures below 180 K already involves the full chemistry of the photoreduction and yields the reaction product, Chlide, in an enzyme-bound form. The subsequent dark reactions, which include cofactor (NADP(+)) release and cofactor (NADPH) rebinding, show different temperature dependences for POR A and POR B and suggest a higher conformational flexibility of POR B in the surrounding active center. Both the higher substrate binding affinity and well adapted enzyme dynamics are held responsible for the increased catalytic activity of POR B as compared with POR A. PMID:26408201

  11. Mxico -Presidencia de la Repblica | Sala de Prensa http://www.presidencia.gob.mx/prensa/?contenido=32287&imprimir=true 1 of 8 10/23/2007 02:29 PM

    E-print Network

    Coello, Carlos A. Coello

    interés por la ciencia por parte de los padres de familia y de algunos maestros es algo determinante. Como sociedad debemos despertar cada día más la curiosidad científica. La escuela debe hacer que las materias de

  12. NaturalezaAragonesa,n.31.ISSN:1138-8013 Aproximadamente una de cada cuatro especies des-

    E-print Network

    Ribera, Ignacio

    ** Los coleópteros acuáticos de Aragón Figura 1. Dytiscus pisanus Laporte de Castelnau, 1834 (Dytiscidae) es Dytiscidae (o ditís- cidos), que se caracterizan por ser buenos nada- dores, por la forma del

  13. TOTAL MUJERES TOTAL MUJERES TOTAL MUJERES MUJERES % MUJERES TOTAL AFGANISTN 22 14 53 26 0 0 40 53,33% 75

    E-print Network

    Autonoma de Madrid, Universidad

    extranjeros por país de nacimiento, ciclo y sexo. PAÍS 1er y 2º CICLO 3er CICLO PROGRAMAS INTERNACIONALES estudiantes extranjeros por país de nacimiento, ciclo y sexo. PAÍS 1er y 2º CICLO 3er CICLO PROGRAMAS

  14. TOTAL MUJERES TOTAL MUJERES TOTAL MUJERES MUJERES % MUJERES TOTAL AFGANISTN 14 6 0 0 1 1 7 46,67% 15

    E-print Network

    Autonoma de Madrid, Universidad

    Origen de los estudiantes: distribución de los estudiantes extranjeros por nacionalidad, ciclo y sexo extranjeros por nacionalidad, ciclo y sexo (2008/09) PAÍS 1er y 2º CICLO 3er CICLO PROGRAMAS INTERNACIONALES

  15. Informe Anual a la Nación sobre el Cáncer de 1975 a 2008: preguntas y respuestas

    Cancer.gov

    Los índices generales de mortalidad por cáncer han seguido disminuyendo desde principios de los noventa entre hombres, mujeres y niños. Los índices de mortalidad disminuyeron en promedio 1,6% por año entre 2004 y 2008.

  16. Curiosidades Meio Ambiente

    E-print Network

    Barbosa, Marcia C. B.

    por um júri presidido pelo vencedor do Prêmio Nobel de Química (1999), Ahmed Zewail, e por Linus Pauling, professor catedrático de Química e Física do Instituto de Tecnologia da Califórnia, nos Estados

  17. 38% dos jovens no tm dinheiro para

    E-print Network

    Instituto de Sistemas e Robotica

    38% dos jovens não têm dinheiro para continuar a estudar Estudantes não são bem informados sobre continuará a estu- dar porque "por um lado, não tem dinheiro e, por outro lado, porque sente que não terá

  18. Introduccin Los ecosistemas acuticos salinos, tanto

    E-print Network

    Murcia, Universidad de

    Introducción Los ecosistemas acuáticos salinos, tanto humedales como ríos, se caracterizan por un los ecosistemas acuáticos salinos, por otro lado, mayoritariamente ibéricos en un contexto europeo EMBLEMÁTICAS DE LA PENÍNSULA IBÉRICA Biodiversidad de macroinvertebrados en los ecosistemas acuáticos salinos

  19. Theory and Applications of Categories, Vol. 11, No. 12, 2003, pp. 283-308. RING EPIMORPHISMS AND C(X)

    E-print Network

    Rosebrugh, Robert

    are assumed to pres* *erve the relevant structures: POR/N , the category of reduced partially ordered is a ring homomorphi* *sm between real closed rings then it is automatically a morphism in POR/N and R

  20. Lifelines Training Researchers PDF

    Cancer.gov

    Diversificación del cuerpo de investigadores de cáncer para ayudar a reducir las desigualdades por  cáncer    Por el Instituto Nacional del Cáncer    En algún lugar, en un laboratorio de investigación, un científico hispano está analizando muestras de 

  1. Degree project in Communication Systems

    E-print Network

    Maguire Jr., Gerald Q.

    redes privadas, controladas y mantenidas por los operadores de telecomunicaciones, por proveedores de. #12;#12;iii Resumen Actualmente, los servicios de telecomunicaciones se implementan comúnmente en implementar servicios de telecomunicaciones en la nube, aprovechando su alta disponibilidad, distribución

  2. Calculo de separadores de grafos utilizando busqueda de vecindad variable reducida

    E-print Network

    Duarte, Abraham

    aplicaciones. Por ejemplo, en redes de telecomunicaciones un separa- dor determina la capacidad y fragilidad de. Por ejemplo, en redes de teleco- municaciones ([15], [13]). En el campo de la algoritmia para grafos

  3. Estudio de NIH señala que la sigmoidoscopia reduce los índices de cáncer colorrectal

    Cancer.gov

    Estudio señala que la sigmoidoscopia flexible es efectiva para reducir los índices de casos nuevos y de muertes por cáncer colorrectal. Los investigadores encontraron que la mortalidad general por cáncer colorrectal se redujo 26% y que la incidencia se re

  4. Tcnicas de programacin -Curso 2008/09 (Esther Guerra Snchez)

    E-print Network

    Guerra, Esther

    proporcionar un medio de navegar por sus datos sin exponer su estructura interna Se debe poder atravesar la lista de varias maneras, pero no añadir operaciones a la lista por cada tipo de recorrido Se debe poder

  5. UNIVERSIDAD DE SANTIAGO DE CHILE FACULTAD DE CIENCIAS

    E-print Network

    académicos, por cualquier forma, medio o procedimiento, siempre y cuando se incluya la cita bibliográfica del esfuerzo que realizaron para poder entregarme todo lo que necesité, por su apoyo incondicional durante

  6. Estimado Padre o Guardin, Le estoy escribiendo porque su hijo o hija ha expresado inters en estudiar en el extranjero

    E-print Network

    Cantlon, Jessica F.

    global política y económica. Su hijo o hija pasará por un proceso definido para poder permanecer un financiera, crear un presupuesto, empacar, transferencia de crédito, y mucho más. Por medio de una buena

  7. Tcnicas de Programacin -Curso 2007/08 Patrones de Diseo

    E-print Network

    Guerra, Esther

    medio de navegar por sus datos sin exponer su estructura interna Se debe poder atravesar la lista de varias maneras, pero no añadir operaciones a la lista por cada tipo de recorrido Se debe poder realizar

  8. C o n f e s i o n e s d e u n a p r e n d i z d e l e x i c g r a f o Jos Antonio Pascual

    E-print Network

    Verschure, Paul

    honran tantos y tan admirados colegas de él--; pero, además, por lo que significa poder contribuir desventuras por las que ha pasado el mundo de los diccionarios en este último largo medio siglo, de las que he

  9. Interfaces CerebroComputador no Invasivos para Sustitucin Motora Dr. Javier Mnguez Zafra

    E-print Network

    Minguez, Javier

    silla de ruedas operada únicamente por el pensamiento y orientada hacia favorecer la movilidad (Iturrate se describe una silla de ruedas robotizada controlada por la actividad cerebral. Aunque actualmente existen una gran cantidad de sillas de ruedas controladas p

  10. 6. GLOSARIO Accin Positiva

    E-print Network

    Fernández Pascual, Ricardo

    niños, independientemente de su sexo. La coeducación es, por tanto, educar desde la igualdad de valores no discriminación por razón de sexo y como principio educativo la formación en igualdad entre los sexos y el rechazo

  11. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    : distribución de estudiantes extranjeros por nacionalidad, ciclo y sexo PAÍS 1er y 2º ciclo 3er ciclo Otros extranjeros por nacionalidad, ciclo y sexo ( cont. ) PAÍS 1er y 2º ciclo 3er ciclo Otros ( * ) TOTAL

  12. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    : distribución de estudiantes extranjeros por nacionalidad, ciclo y sexo PAÍS 1er y 2º ciclo 3er ciclo Programas estudiantes: distribución de estudiantes extranjeros por nacionalidad, ciclo y sexo PAÍS 1er y 2º ciclo 3er

  13. INFORME MUJERES INVESTIGADORAS 2012 INTRODUCCION AL INFORME "Mujeres Investigadoras 2012"

    E-print Network

    Fitze, Patrick

    SEXO/RELACIÓN LABORAL......... 3 GRÁFICA "TIJERA" PERSONAL INVESTIGADOR ................................ 3 EDAD MEDIA DEL PERSONAL CIENTÍFICO POR SEXO........................ 4 DISTRIBUCIÓN DEL PERSONAL INVESTIGADOR CONTRATADO Y BECADO ..................... 10 PERSONAL PRE-DOCTORAL EN FORMACIÓN POR ÁREAS Y SEXO

  14. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    : distribución de estudiantes extranjeros por nacionalidad, ciclo y sexo PAÍS 1er y 2º ciclo 3er ciclo Otros estudiantes extranjeros por nacionalidad, ciclo y sexo ( cont. ) PAÍS 1er y 2º ciclo 3er ciclo Otros

  15. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    : distribución de estudiantes matriculados y titulados en el curso 2000/01 por títulos y sexo TÍTULO MATRICULADOS 00/01 TITULADOS 00/01 #12;Distribución de los estudiantes de Títulos Propios por sexo 1259 695 1119

  16. MANIFIESTO DE LA RED DE UNIDADES DE IGUALDAD DE GNERO DE LAS UNIVERSIDADES ESPAOLAS PARA LA EXCELENCIA

    E-print Network

    acoso sexual, el acoso por razón de sexo, la trata de mujeres y niñas. La Universidad, como cualquier, como el acoso sexual o el acoso por razón de sexo, formas de violencia hacia las mujeres que #12;causan

  17. CENTRO PLAN TITULACIN TOTAL MUJERES 41 Licenciado en Qumica 38 23

    E-print Network

    Autonoma de Madrid, Universidad

    titulaciones de centros propios en el curso académico 2012 / 2013, por centro, plan de estudios y sexo el curso académico 2012 / 2013, por centro, plan de estudios y sexo (continuación) TOTAL FORMACIÓN

  18. PLAN DE IGUALDAD UNIVERSIDAD COMPLUTENSE DE MADRID

    E-print Network

    aún subsistentes de discriminación, directa o indirecta, por razón de sexo y a promover la igualdad directa e indirecta, en el acoso sexual y por razón de sexo, y en la necesidad de acciones positivas. El

  19. 76 FR 26241 - Certain Frozen Warmwater Shrimp From Thailand: Notice of Court Decision Not in Harmony With Final...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2011-05-06

    ...Certain Frozen Warmwater Shrimp From Thailand: Notice of Court Decision Not in Harmony...certain frozen warmwater shrimp from Thailand covering the period of review (POR...certain frozen warmwater shrimp from Thailand covering the POR of February 1,...

  20. Departamento de Artes & Design Programa de Ps-graduao em Arquitetura

    E-print Network

    Díaz, Lorenzo J.

    enviado por Sedex ou courrier (Fedex ou similar), com comprovante de postagem e entrega. 1.7. Recomendação mesmo horário por Fedex, courrier ou similar). Alternativamente, os formulários podem ser pree

  1. Instituto Nacional de Pesquisas Espaciais Av. dos Astronautas, 1758 So Jos dos Campos, SP.

    E-print Network

    (quantidade de energia por unidade de tempo) emitida por uma lâmpada incandescente. Para isso, utiliza um tempo de vida médio destas partículas ? 9) Uma nave viajando de encontro à Terra a uma velocidade igual

  2. Universidade Estadual de Campinas Instituto de Matemtica, Estatstica e Computao Cientfica

    E-print Network

    Gomes, Francisco A. M.

    -Graduação: Mestrado em Matemática Aplicada #12;iii #12;v #12;vii AGRADECIMENTOS Agradeço primeiramente a DEUS por a DEUS por ter uma família tão maravilhosa que sempre me apoiou em meus estudos, mesmo não entendendo

  3. Interview: Somos los sobrevivientes de una utopía': Entrevista a Adhemar Bianchi

    E-print Network

    Pellarolo, Silvia; Proañ o-Gó mez, Lola

    2001-10-01

    Teatro Circular y en la escuela municipal de 118 LATIN AMERICAN THEATRE REVIEW arte dramático de Montevideo en Uruguay, donde los planes de estudio comenzaban por el teatro griego y llegaban al teatro contemporáneo, pasando por los latinos, la comedia... nada menos que teatro popular. ¿No pasaste por el teatro independiente? Sí, cuando salí de la escuela me integré al Teatro Circular de Montevideo, donde fui actor, director y miembro de su consejo directivo hasta el año 1973, cuando por causas de...

  4. Festival Report: Caracas, Tercer Festival Internacional de Teatro

    E-print Network

    Rozental, Geneviè ve

    1976-10-01

    rasgos ya conocidos; talento pero premura y falta de continuidad de los elencos. Vimos por una parte un teatro de textos, algo clásico, profesionalizado, representado funda mentalmente por el Nuevo Grupo de Chocrón, Chalbaud y Cabrujas; y por otra... su talento sino por su valentía. También se celebra la belleza misteriosa de los espectáculos que presenta, verdaderos tests de Rorschach pretendiendo provocar en el espectador una conmoción íntima. Come and the Day will be Ours, creación colectiva...

  5. PRESIDNCIA DA REPBLICA CONTROLADORIA-GERAL DA UNIO

    E-print Network

    Maier, Rudolf Richard

    encargo de curso ou concurso a servidor por meio de ordem bancária, o que contraria o disposto no art. 9º remunerações. 3.1.1.5 Fracionamento de despesas por meio de dispensa de licitação - CDT 3.1.1.6 Fracionamento de despesas por meio de dispensa de licitação - CESPE 3.1.1.7 Fracionamento de despesas por meio de

  6. Analise Matematica III Exerccios

    E-print Network

    Godinho, Leonor

    S por meio de um integral duplo sobre S e tambâ??em por meio de um integral duplo sobre T . d) Calcule Z Z S 1 (x - y + 1) 2 dxdy. 1 #12; 6 Mostre por meio de uma mudanâ?ºca de coordenadas apropriada que Z Z S f(x + y)dxdy = Z 1 -1 f(u)du onde S = {(x, y) # R 2 : |x| + |y| # 1}. 7 Mostre por meio de uma mudanâ?ºca de

  7. LO SABA?... Jobbagy, Mercau & Nosetto

    E-print Network

    Nacional de San Luis, Universidad

    zona saturada (todos los poros ocupados por agua) del perfil de un suelo/sedimento. Los hidrólogos las Verdadera. ZONA CAPILAR 1 El agua asciende sobre la altura de la napa por los capilares del suelo. En.0-1.5 m por sobre la napa. En suelos más arenosos hasta 0.5-1.0 m. En suelos más arcillosos la tasa de

  8. Pgina 1 de 20 1. ANTECEDENTES

    E-print Network

    Función Pública (SFP), del 2 al 13 de septiembre de 2013, el Cinvestav aplicó por quinta vez la encuesta por medio del sistema habilitado en internet por la SFP. Un total de 184 trabajadores la requisitaron diferentes. El 6 de noviembre de 2013, la SFP celebró la "Reunión de Entrega de Resultados de la Encuesta de

  9. Superficies y Vaco 15, 9-12, diciembre de 2002 Sociedad Mexicana de Ciencia de Superficies y de Vaco Estudio de pelculas delgadas de Cd1-x Znx Te, para 0

    E-print Network

    Meléndez Lira, Miguel Angel

    libre (CSVT-FE, por sus siglas en ingles) usando la coevaporación de CdTe y ZnTe. La incorporación del, tales como Epitaxia de haces moleculares (MBE, por sus siglas en ingles) [5], y deposito químico de metalorgánicos al vacío (MOCVD, por sus siglas en ingles) [6]. Utilizando técnicas baratas, tales como transporte

  10. Page 1 of 8 Universidad De Carolina Del Este

    E-print Network

    Gopalakrishnan, K.

    Abril del 2003 REVISADO: 13 de Octubre del 2014 En ECU Physicians (por sus siglas en ingles ECUP) y. Este Aviso se aplica a toda su información médica protegida ( PHI por sus siglas en ingles) que REVELAR SU INFORMACION MEDICA PROTEGIDA (PHI POR SUS SIGLAS EN INGLES ) SIN SU AUTORIZACION EN LOS

  11. MAPA DE RIESGO DE EROSIN DE SUELO PARA LA CUENCA DEL RIO YAGEZ EN PUERTO RICO

    E-print Network

    Gilbes, Fernando

    , RUSLE (por sus siglas en ingles), es comúnmente utilizada para la estimación anual de pérdida de suelos-RUSLE (por sus siglas en ingles). Objetivos del Estudio El principal objetivo del presente estudio fue utilizando el modelo de pérdida de suelos universal revisado (RUSLE) por sus siglas en ingles, para

  12. Ao IV, Vol. II. Julio -Diciembre 2012 Columnas Komputer Sapiens 29 / 32 Deskubriendo Konocimiento

    E-print Network

    Grimaldo, Francisco

    la teor´ia de sis- temas y del funcionalismo estructu- ral, la simulaci´on por computador se ha usado´on conducen a establecer un cla- ro paralelismo con la teor´ia de los sistemas complejos. Por ´ultimo, co- mo prosocial. Por ejemplo, cuando se trata de juegos competitivos con interaccio- nes en pareja, como en el

  13. Revised: 2/26/2014 Yale University

    E-print Network

    Post, David M.

    con mi proveedor/a del cuidado de salud para revisar SOLAMENTE la información que describí arriba licenciado proveedor profesional del cuidado de salud designado por Yale, sometiendo mi petición por escrito una queja por escrito a la Oficina del Departamento de salud y servicios humanos de Estados Unidos de

  14. PROGRAMA DE CAPACITACIN Y EDUCACIN EN SEGURIDAD Y SALUD PARA TRABAJADORES COMISIN DE SALUD Y SEGURIDAD Y DE COMPENSACIN AL TRABAJADOR

    E-print Network

    Leistikow, Bruce N.

    PROGRAMA DE CAPACITACIÓN Y EDUCACIÓN EN SEGURIDAD Y SALUD PARA TRABAJADORES COMISIÓN DE SALUD Y Lecherías ha sido desarrollado por el Centro Occidental de Salud y Seguridad Agrícola (WCAHS por sus siglas Educación en Salud y Seguridad para Trabajadores (WOSHTEP por sus siglas en inglés). WOSHTEP es administrado

  15. Light-Dependent Protochlorophyllide Oxidoreductase: Phylogeny, Regulation, and Catalytic Properties.

    PubMed

    Gabruk, Michal; Mysliwa-Kurdziel, Beata

    2015-09-01

    This Current Topic focuses on light-dependent protochlorophyllide oxidoreductase (POR, EC 1.3.1.33). POR catalyzes the penultimate reaction of chlorophyll biosynthesis, i.e., the light-triggered reduction of protochlorophyllide to chlorophyllide. In this reaction, the chlorin ring of the chlorophyll molecule is formed, which is crucial for photosynthesis. POR is one of very few enzymes that are driven by light; however, it is unique in the need for its substrate to absorb photons to induce the conformational changes in the enzyme, which are required for its catalytic activation. Moreover, the enzyme is also involved in the negative feedback of the chlorophyll biosynthesis pathway and controls chlorophyll content via its light-dependent activity. Even though it has been almost 70 years since the first isolation of active POR complexes, our knowledge of them has markedly advanced in recent years. In this review, we summarize the current state of knowledge of POR, including the phylogenetic roots of POR, the mechanisms of the regulation of POR genes expression, the regulation of POR activity, the import of POR into plastids, the role of POR in PLB formation, and the molecular mechanism of protochlorophyllide reduction by POR. To the best of our knowledge, no previous review has compiled such a broad set of recent findings about POR. PMID:26230427

  16. Repaso de matrices, determinantes y sistemas de ecuaciones lineales

    E-print Network

    Hurtado, Ana

    ciento de nitr´ogeno, un 21 por ciento de ox´igeno y un 1 por ciento de arg´on. Para poder realizar un forma de rect´angulo, que usualmente se delimitan por medio de par´entesis. Si una matriz tiene n filas

  17. PRE SE NTACIN PARANINFO DE LA UNIVERSIDAD POLITCNICA DE MADRID

    E-print Network

    Cardeñosa, Jesús

    .00 HORAS. C/RAMIRO DE MAEZTU, 7 "GUÍA PARA LA PREVENCIÓN DEL ACOSO SEXUAL, ACOSO POR RAZÓN DE SEXO Y ACOSO "GUÍA PARA LA PREVENCIÓN DEL ACOSO SEXUAL, ACOSO POR RAZÓN DE SEXO Y ACOSO PSICOLÓGICO" 17 DE NOVIEMBRE Sánchez de Madariaga "Guía para la prevención del acoso sexual, acoso por razón de sexo y acoso

  18. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    de 2003 #12;Tabla 72. Tutoría individualizada: distribución de estudiantes por titulación y sexo 2002) DIPLOMATURA Ed. Social 1 0 1 Usuarios/as por titulación y sexo Enfermería 4 0 4 Magisterio 9 4 13 Orientación e Información para el Empleo, a 4 de Mayo de 2003 Tutorías Individualizadas por Sexo MUJERES 68

  19. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    Estudiantes #12;4.3. Perfiles de los Estudiantes 4.3.1. Distribución de los estudiantes por sexo y edad Fuente: SIGMA, a 2 de Junio de 2002 Fuente: SIGMA, a 2 de Junio de 2002 Distribución de estudiantes por sexo en ercado Lingüística M atem áticas M edicina Psicología #12;Distribución de estudiantes por sexo en 3er

  20. Hogares, medios de vida y recursos naturales en reas rurales de

    E-print Network

    Columbia University

    , estructura por sexo y edad, dinámica interna (relaciones entre los miembros, derechos y deberes, decisiones; ­ Los eventos del ciclo de vida del hogar (nacimientos, muertes), al afectar la estructura por sexo y terminos de derechos y obligaciones de acuerdo a la composicion por sexo y edad, y estan asimetrias pueden

  1. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    Estudiantes #12;4.3. Perfiles de los Estudiantes 4.3.1. Distribución de los estudiantes por sexo y edad Tabla 13. Estudiantes de 1er y 2º Ciclo distribuidos por titulación y sexo NIVEL TITULACIÓN HOMBRES MUJERES Distribución de estudiantes por sexo en 1er y 2º ciclo MUJERES 61% HOMBRES 39% #12;Porcentaje de mujeres en

  2. ACCIN HORIZONTAL DE IGUALDAD DE GNERO EN LA AGENCIA ESTATAL CSIC

    E-print Network

    Fitze, Patrick

    de los poderes públicos de promover las condiciones para que la igualdad por razón de sexo sea real y visión por escalones de personal científico desglosado por sexos, y cierta referencia al progreso todos sus estamentos, al menos el 40% del sexo menos representado en el área), en tanto que el avance en

  3. BOLETN OFICIAL DEL ESTADO Nm. 189 Lunes 8 de agosto de 2011 Sec. I. Pg. 90134

    E-print Network

    Fitze, Patrick

    Protocolo de actuación frente al acoso sexual y al acoso por razón de sexo en el ámbito de la Administración desarrollo de la personalidad (artículo 10.1), la igualdad y la no discriminación por razón de sexo en no discriminación por razón de sexo en el ámbito de las relaciones de trabajo. Además en su artículo 9.2 dispone que

  4. 75 FR 42485 - Additional Designation of Entities and Individuals Pursuant to Executive Order 12978

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-07-21

    ...ARISTIZABAL MEJIA, Diego, c/o BOSQUES DE AGUA SOCIEDAD POR ACCIONES SIMPLIFICADA, Medellin...MOLINA, Luis Bernardo, c/o BOSQUES DE AGUA SOCIEDAD POR ACCIONES SIMPLIFICADA, Medellin...INDIVIDUAL) [SDNT]. Entities 1. BOSQUES DE AGUA SOCIEDAD POR ACCIONES SIMPLIFICADA,...

  5. REGRAS PARA INGRESSO E PERMANNCIA NO CORPO DOCENTE DA CAP INGRESSO NO CORPO DOCENTE

    E-print Network

    interessado em ingressar no corpo docente do curso CAP deverá manifestar seu interesse por meio de: - título de doutor reconhecido pelo MEC - ter pelo menos a pontuação de 1,0, no último ano, por meio de, por meio de publicações em periódicos classificados no QUALIS. A pontuação está apresentada na Tabela

  6. Marti?nez Sierra

    E-print Network

    Gidinghagen, Myrtle Rosalie

    1920-01-01

    . This quality of intellectual 36 emotion is rather new in Spanish literature, one which forms a link between the poets of the present generation, like •*3 Ruben Dario, Santiago Rusinol, Juan Ramon Jimenez and Eduardo Marquina. As Martinez Sierra expresses..., Martinez Sierra more fully explains this term:"Por este 'sonar no se con que* de Antonio Machado; por este *llorar por nadie y por alguien' de Juan Ramon Jimenez; por la tragica lamentaoi6n sobre la juventud que huye, de Ruben 1* El agua dorm Ida, p. 19...

  7. Drinking Water Problems: Lead (Spanish) 

    E-print Network

    Dozier, Monty; McFarland, Mark L.

    2004-02-20

    ? expuesto quiz?s provenga del agua potable; sin embargo, los ni?os que son alimentados con f?rmula podr?an obtener del 40 al 60 por ciento de su consumo de plomo del agua. La ?nica manera de detectar el envenenamiento por plomo es por medio de una prueba...?n, deter- mine si alg?n equipo el?ctrico (incluyendo tel?- fonos) ha sido conectado por cables subterr?neos cercanos a la plomer?a. La corriente el?ctrica que viaja por los cables en el suelo acelera la corrosi?n en las ca?er?as. Necesitar? llamar a un...

  8. Altered heme catabolism by heme oxygenase-1 caused by mutations in human NADPH cytochrome P450 reductase

    SciTech Connect

    Pandey, Amit V.; Flueck, Christa E.; Mullis, Primus E.

    2010-09-24

    Research highlights: {yields} Mutations in POR identified from patients lead to reduced HO-1 activities. {yields} POR mutation Y181D affecting FMN binding results in total loss of HO-1 activity. {yields} POR mutations A287P, C569Y and V608F, lost 50-70% activity. {yields} Mutations in FAD binding domain, R457H, Y459H and V492E lost all HO-1 activity. {yields} POR polymorphisms P228L, R316W, G413S, A503V and G504R have normal activity. -- Abstract: Human heme oxygenase-1 (HO-1) carries out heme catabolism supported by electrons supplied from the NADPH through NADPH P450 reductase (POR, CPR). Previously we have shown that mutations in human POR cause a rare form of congenital adrenal hyperplasia. In this study, we have evaluated the effects of mutations in POR on HO-1 activity. We used purified preparations of wild type and mutant human POR and in vitro reconstitution with purified HO-1 to measure heme degradation in a coupled assay using biliverdin reductase. Here we show that mutations in POR found in patients may reduce HO-1 activity, potentially influencing heme catabolism in individuals carrying mutant POR alleles. POR mutants Y181D, A457H, Y459H, V492E and R616X had total loss of HO-1 activity, while POR mutations A287P, C569Y and V608F lost 50-70% activity. The POR variants P228L, R316W and G413S, A503V and G504R identified as polymorphs had close to WT activity. Loss of HO-1 activity may result in increased oxidative neurotoxicity, anemia, growth retardation and iron deposition. Further examination of patients affected with POR deficiency will be required to assess the metabolic effects of reduced HO-1 activity in affected individuals.

  9. Efficient anti-tumor effect of photodynamic treatment with polymeric nanoparticles composed of polyethylene glycol and polylactic acid block copolymer encapsulating hydrophobic porphyrin derivative.

    PubMed

    Ogawara, Ken-Ichi; Shiraishi, Taro; Araki, Tomoya; Watanabe, Taka-Ichi; Ono, Tsutomu; Higaki, Kazutaka

    2016-01-20

    To develop potent and safer formulation of photosensitizer for cancer photodynamic therapy (PDT), we tried to formulate hydrophobic porphyrin derivative, photoprotoporphyrin IX dimethyl ester (PppIX-DME), into polymeric nanoparticles composed of polyethylene glycol and polylactic acid block copolymer (PN-Por). The mean particle size of PN-Por prepared was around 80nm and the zeta potential was determined to be weakly negative. In vitro phototoxicity study for PN-Por clearly indicated the significant phototoxicity of PN-Por for three types of tumor cells tested (Colon-26 carcinoma (C26), B16BL6 melanoma and Lewis lung cancer cells) in the PppIX-DME concentration-dependent fashion. Furthermore, it was suggested that the release of PppIX-DME from PN-Por would gradually occur to provide the sustained release of PppIX-DME. In vivo pharmacokinetics of PN-Por after intravenous administration was evaluated in C26 tumor-bearing mice, and PN-Por exhibited low affinity to the liver and spleen and was therefore retained in the blood circulation for a long time, leading to the efficient tumor disposition of PN-Por. Furthermore, significant and highly effective anti-tumor effect was confirmed in C26 tumor-bearing mice with the local light irradiation onto C26 tumor tissues after PN-Por injection. These findings indicate the potency of PN-Por for the development of more efficient PDT-based cancer treatments. PMID:26593985

  10. Perceptions of risks to children's health from indoor air pollution and an investigation of respiratory illness symptoms from indoor heating with a woodburning stove

    SciTech Connect

    Osborne, J.S. III

    1986-01-01

    This study explores perceptions of risks to health (POR), risk taking behavior (RTB), and associated illness from the perspectives of medical anthropology and epidemiology. Specific objectives included investigation of the following: (1) general POR: (2) POR from indoor air pollution (IAP) and parental usage of IAP sources; (3) the relationship between POR and RTB; factors affecting POR and RTB; (4) the construction of risk domains; (5) sources of intracultural variation in POR, RTB, and the partitioning of risk domains; (6) patterns of indoor heating in central Michigan; and (7) respiratory illness symptoms (RIS) in children from exposure to indoor heating with a woodburning stove (WBS). POR and RTB were investigated in random and ethnographic samples, RIS in a random sample with an internal comparison group, and indoor heating in a random sample. Interview schedules were developed for each phase of inquiry. The instrument used to investigate POR and RTB includes both qualitative and quantitative scales for POR assessment. Findings of this study provide an ethnography of risk, an inferential analysis of POR/RTB, and a preliminary assessment of risks of RIS from heating with WBS.

  11. Patterns of coral disease across the Hawaiian Archipelago: Relating disease to environment

    USGS Publications Warehouse

    Aeby, G.S.; Williams, G.J.; Franklin, E.C.; Kenyon, J.; Cox, E.F.; Coles, S.; Work, T.M.

    2011-01-01

    In Hawaii, coral reefs occur across a gradient of biological (host abundance), climatic (sea surface temperature anomalies) and anthropogenic conditions from the human-impacted reefs of the main Hawaiian Islands (MHI) to the pristine reefs of the northwestern Hawaiian Islands (NWHI). Coral disease surveys were conducted at 142 sites from across the Archipelago and disease patterns examined. Twelve diseases were recorded from three coral genera (Porites, Montipora, Acropora) with Porites having the highest prevalence. Porites growth anomalies (PorGAs) were significantly more prevalent within and indicative of reefs in the MHI and Porites trematodiasis (PorTrm) was significantly more prevalent within and indicative of reefs in the NWHI. Porites tissue loss syndrome (PorTLS) was also important in driving regional differences but that relationship was less clear. These results highlight the importance of understanding disease ecology when interpreting patterns of disease occurrence. PorTrm is caused by a parasitic flatworm that utilizes multiple hosts during its life cycle (fish, mollusk and coral). All three hosts must be present for the disease to occur and higher host abundance leads to higher disease prevalence. Thus, a high prevalence of PorTrm on Hawaiian reefs would be an indicator of a healthy coral reef ecosystem. In contrast, the high occurrence of PorGAs within the MHI suggests that PorGAs are related, directly or indirectly, to some environmental co-factor associated with increased human population sizes. Focusing on the three indicator diseases (PorGAs, PorTrm, PorTLS) we used statistical modeling to examine the underlying associations between disease prevalence and 14 different predictor variables (biotic and abiotic). All three diseases showed positive associations with host abundance and negative associations with thermal stress. The association with human population density differed among disease states with PorGAs showing a positive and PorTrm showing a negative association, but no significant explanatory power was offered for PorTLS. ?? 2011 Aeby et al.

  12. High-Yield Expression of a Catalytically Active Membrane-Bound Protein: Human P450 Oxidoreductase

    PubMed Central

    Sandee, Duanpen

    2011-01-01

    P450 oxidoreductase (POR) is a two-flavin protein that reduces microsomal P450 enzymes and some other proteins. Preparation of active bacterially expressed human POR for biochemical studies has been difficult because membrane-bound proteins tend to interact with column matrices. To reduce column-protein interactions and permit more vigorous washing, human POR lacking 27 N-terminal residues (N-27 POR) was modified to carry a C-terminal Gly3His6-tag (N-27 POR-G3H6). When expressed in Escherichia coli, N-27 POR-G3H6 could be purified to apparent homogeneity by a modified, single-step nickel-nitrilotriacetic acid affinity chromatography, yielding 31 mg POR per liter of culture, whereas standard purification of native N-27 POR required multiple steps, yielding 5 mg POR per liter. Both POR proteins had absorption maxima at 375 and 453 nm and both reduced cytochrome c with indistinguishable specific activities. Using progesterone as substrate for bacterially expressed purified human P450c17, the Michaelis constant for 17?-hydroxylase activity supported by N-27 POR or N-27 POR-G3H6 were 1.73 or 1.49 ?m, and the maximal velocity was 0.029 or 0.026 pmol steroids per picomole P450 per minute, respectively. Using 17-hydroxypregnenolone as the P450c17 substrate, the Michaelis constant for 17,20 lyase activity using N-27 POR or N-27 POR-G3H6 was 1.92 or 1.89 ?m and the maximal velocity was 0.041 or 0.042 pmol steroid per picomole P450 per minute, respectively. Thus, N-27 POR-G3H6 is equally active as native N-27 POR. This expression and purification system permits the rapid preparation of large amounts of highly pure, biologically active POR and may be generally applicable for the preparation of membrane-bound proteins. PMID:21586563

  13. El Teorema Pi y la modelacion Luis Quintanar Medina

    E-print Network

    Vela-Arevalo, Luz V.

    realidad y reducir costos de experimentaci´on; esta t´ecnica es muy ´util en problemas que surgen en mec´anica, en particular la de fluidos. Las magnitudes como la velocidad, densidad, etc. se expresan en ciertas unidades, como metros por segundo m s , kilogramo por metro c´ubico kg m3 , etc. Por otra parte, en mec´anica

  14. Boletin de la Sociedad de Estadistica e Investigacion Operativa (2013), XX, X, XXX-XXX.

    E-print Network

    Gómez Villegas, Miguel Ángel

    2013-01-01

    Bolet´in de la Sociedad de Estad´istica e Investigaci´on Operativa (2013), XX, X, XXX-XXX. GEORGE E. P. BOX (1919-2013) OBITUARIO por Miguel A. G´omez Villegas Dpto. de Estad´istica e Investigaci gobierno brit´anico por no hacer nada para parar a Hitler. Descubri´o la estad´istica por accidente, de

  15. Jorge Enrique Adoum en París.

    E-print Network

    Rozental, Geneviè ve

    1975-04-01

    americanos. Su espectáculo es poco costoso, porque lo realizó con unas cuantas cajas pintadas de negro, algunos metros de tela roja y unos objetos—¡magníficos! —dibujados por Oswaldo Guayasamin en Quito y fabricados en París por Fran chise Darne. Los... efectos logrados con las luces y tan poco material son notables. Las cajas, dispuestas o apiladas de diversos modos, pueden ser barco, isla, trono, cárcel, etc. . . . Las manos de los prisioneros que salen retorciéndose por los agujeros especiales de...

  16. Diario de Sevilla, 16 de febrero de 2005 20-F: ni alarmas ni dramatismos

    E-print Network

    Bilbao Arrese, Jesús Mario

    activo la siguiente pregunta: ¿Aprueba usted el Tratado por el que se establece una Constitución para Europa? Esta pregunta se refiere al proyecto de Tratado adoptado por unanimidad el 18 de junio de 2004 de octubre de 2004, se procedió en Roma a la firma del Tratado, por lo que su aprobación está

  17. UNIVERSIDAD DE BUENOS AIRES SECRETARA DE ASUNTOS ACADMICOS

    E-print Network

    Cortiñas, Guillermo

    . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 Estudiantes según Unidad Académica de cursado y sexo por Unidad Académica a la que pertenece la.1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 Estudiantes según Unidad Académica de cursado de la carrera y sexo por carrera cursada -Facultad.2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71 Estudiantes según Unidad Académica de cursado de la carrera y sexo por carrera cursada -Facultad

  18. Lo uno y lo múltiple: Farsa e incesto en Quíntuples de Sánchez

    E-print Network

    Melé ndez, Priscilla

    1992-10-01

    de Sánchez trata precisamente de la familia Morrison, compuesta por el Gran Divo Papá Morrison y sus quintuples Dafne, Baby, Bianca, Mandrake y Carlota, que ha sido invitada a participar en el Congreso de Asuntos de la Familia. Cada uno de ellos se... respectivos papeles: Francisco Prado recorre la gama desde la 'timidez' fantasiosa de 'Baby Morrison', pasando por la engreída y altanera personalidad de 'Mandrake Morrison' hasta llegar al desbordante y lujurioso 'Papá'. Por su parte, Idalia Pérez Garay...

  19. Programa de Ps-Graduao UNIVERSIDADE DE BRASLIA

    E-print Network

    Maier, Rudolf Richard

    , a saber: a) ingresso universal; b) ingresso por meio da modalidade de reserva de vagas para candidato(a)s negro(a)s; e c) ingresso por meio da modalidade de reserva de vagas para candidato(a)s indígenas. 1 Ação Afirmativa da UnB. 3.2 A adesão a esta modalidade se dará de forma voluntária por meio do

  20. El Teatro Municipal de Asunción, Paraguay: Historia y reflexión

    E-print Network

    Rí os, Edda de los

    1987-10-01

    habitantes luchaban denodadamente por reconstruir la familia y el país, albergara una importante sala Teatral, que recibió el nombre de Teatro Nacional y cuyos gastos fueron íntegramente cos teados por un grupo de familias que lo legaban a la comunidad... recorrían mundo en incansables giras, sin pasar de largo por Asunción, pequeña pero interesante plaza de público al que no podía dársele "gato por liebre" y que aseguraba a las compañías de repertorio un mes de tem porada a teatro lleno. En esa escuela...

  1. The Exploration and Preliminary Colonization of the Seno Mexicano under don José de Escandón (1747-1749): An Analysis Based on Primary Spanish Manuscripts 

    E-print Network

    Cunningham, Debbie S.

    2011-10-21

    , ofreze ya el arroyo mucho hondable, de forma que no puede sacarse por- zion de dho metal por el gran trabajo que da. ? El dia tres de el corriente mes de marzo, reco- nozi el Lago de la Sanctissima Trinidad, distant- 30 te siete leguas... supliesen algo la nezesidad desde 5 que entre en el monte de este rio, cuia lati- tud es por esta parte diez y ocho leguas que obligan a grandes rodeos por sus muchas malezas. ? El dia ocho de el corr[ient]e, llegue a este paraje 10 de el...

  2. Complicaciones gastrointestinales (PDQ)—Versión para pacientes

    Cancer.gov

    Resumen de información revisada por expertos acerca del estreñimiento, impacción, obstrucción intestinal y diarrea como complicaciones del cáncer o su tratamiento. Se discute el manejo de estos problemas.

  3. La recontextualización de Antígona en el teatro argentino y brasileño a partir de 1968

    E-print Network

    Moreno, Iani del Rosario

    1997-04-01

    expone por medio de las técnicas del teatro épico. En la obra, Marta, acompañada por Quitéria (la amante de su hijo), decide pedirle permiso a alguna de las congregaciones (cofrarias) del pueblo para poder enterrar a José en cualquiera de los... de este país a la vez que se invierte la posición de poder de sus personajes. Esto se logra por medio del humor, elpastiche y los juegos de poder. Antígona furiosa cuestiona el poder de su tío, o sea las leyes de los hombres. También por medio de...

  4. Aspectos do Teorema Fundamental do Calculo

    E-print Network

    Liu, I-Shih

    velocidade e acelera¸c~ao por meio da no¸c~ao de espa¸co percorrido por uma part´icula. Adota-se a nota Fase Leibniz (1646-1716) Pensava no c´alculo de ´areas por meio da no¸c~ao de limite obtendo um novo Considere-se uma fun¸c~ao cont´inua f : [a, b] R, uma parti¸c~ao P de [a, b] por meio dos pontos: a = x0

  5. Neoplasias mielodisplásicas o mieloproliferativas (PDQ)—Versión para pacientes

    Cancer.gov

    Resumen de información revisada por expertos acerca del tratamiento de las neoplasias mielodisplásicas o mieloproliferativas, incluso las leucemias mielomonocíticas crónicas o juveniles, y la LMC atípica.

  6. UNIVERSIDAD SIMON BOLIVAR Decanato de Estudios Profesionales

    E-print Network

    Vásquez, Carlos

    Vicente (Coordinador de Arquitectura), Susana Curbelo (Coordinadora de Ingeniería Química), Lenys por las evaluaciones que no han podido realizarse. El Prof. Henry Vicente, Coordinador de Arquitectura

  7. Inhibition of the Alternative Pathway of Nonhuman Infant Complement by Porin B2 Contributes to Virulence of Neisseria meningitidis in the Infant Rat Model

    PubMed Central

    Vu, David M.; Granoff, Dan M.; Ram, Sanjay

    2014-01-01

    Neisseria meningitidis utilizes capsular polysaccharide, lipooligosaccharide (LOS) sialic acid, factor H binding protein (fHbp), and neisserial surface protein A (NspA) to regulate the alternative pathway (AP) of complement. Using meningococcal mutants that lacked all four of the above-mentioned molecules (quadruple mutants), we recently identified a role for PorB2 in attenuating the human AP; inhibition was mediated by human fH, a key downregulatory protein of the AP. Previous studies showed that fH downregulation of the AP via fHbp or NspA is specific for human fH. Here, we report that PorB2-expressing quadruple mutants also regulate the AP of baby rabbit and infant rat complement. Blocking a human fH binding region on PorB2 of the quadruple mutant of strain 4243 with a chimeric protein that comprised human fH domains 6 and 7 fused to murine IgG Fc enhanced AP-mediated baby rabbit C3 deposition, which provided evidence for an fH-dependent mechanism of nonhuman AP regulation by PorB2. Using isogenic mutants of strain H44/76 that differed only in their PorB molecules, we confirmed a role for PorB2 in resistance to killing by infant rat serum. The PorB2-expressing strain also caused higher levels of bacteremia in infant rats than its isogenic PorB3-expressing counterpart, thus providing a molecular basis for increased survival of PorB2 isolates in this model. These studies link PorB2 expression with infection of infant rats, which could inform the choice of meningococcal strains for use in animal models, and reveals, for the first time, that PorB2-expressing strains of N. meningitidis regulate the AP of baby rabbits and rats. PMID:24686052

  8. Lista de Problemas da Area II 1. Modelo de Blume-Capel I (Salinas): Considere um modelo unidimensional

    E-print Network

    Stariolo, Daniel Adrián

    N 2 , u( , )f( )f( ) e S NkB = - f() ln f() respectivamente. Minimizando a energia livre U - TS . 6. Ponto tricrítico (Pathria): Considere um sistema descrito por uma energia livre de Landau da transferência. (b) Calcule expressões para a energia interna e a entropia por spin. (c) Qual o estado

  9. Ports on the Texas Gulf Coast -- Economic Importance and Role for Texas and U.S. Grain Crops. 

    E-print Network

    Johnson, Edward Mitch; Fuller, Stephen

    1980-01-01

    n a l maritime system v i a the por t e levator , the nexus between these two t ranspor ta t ion systems. The por t e l eva to r is the v i t a l l i n k between these two e s s e n t i a l systems of t ranspor t and must orches...

  10. UNIVERSIDAD DE ALCAL Departamento de Automtica

    E-print Network

    Rodríguez, Inmaculada

    grado de doctor Inmaculada Rodríguez Santiago Dirigida por: Dr. Ronan Boulic. Escuela Politécnica diseños utilizados. A Paolo Baerlocher por su ayuda en todo momento durante mis estancias en la Escuela experimentos que hemos realizado. A Carmen Fernández y toda su familia, ellos han hecho mas llevadero el hecho

  11. Sumarios del PDQ® de información sobre el cáncer

    Cancer.gov

    Los sumarios del PDQ de información de cáncer proveen información revisada por expertos con base en comprobación científica sobre tópicos que incluyen el tratamiento de cáncer en adultos y niños, cuidados médicos de apoyo, prevención, exámenes de detección, genética y medicina complementaria y alternativa, por lo general en formato para pacientes y profesionales de salud.

  12. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    extranjeros por país de nacimiento, ciclo y sexo PROGRAMAS PAÍS 1er y 2º CICLO 3er CICLO INTERNACIONALES TOTAL extranjeros por país de nacimiento, ciclo y sexo PROGRAMAS PAÍS 1er y 2º CICLO 3er CICLO INTERNACIONALES TOTAL

  13. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    de la UAM, a 1 de julio de 2004 Comparativa por sexos Hombres 29% Mujeres 71% 45 7 20 36 1 8 27 2 0 Solidaria y Cooperación, distribuidos por facultad / escuela, programa de voluntariado y sexo Facultades y escuelas Sexo Voluntariado Ambiental Voluntariado Apoyo a Mayores Voluntariado de Animación en Hospitales

  14. Comunicado de prensa del Informe Anual sobre el Estado del Cáncer 2015

    Cancer.gov

    Por primera vez, los investigadores han usado datos a nivel nacional para determinar la incidencia de los cuatro subtipos moleculares principales de cáncer de seno (mama) por edad, raza y grupo étnico, nivel de pobreza y varios otros factores. El informe

  15. Direccin de correo (en letra de imprenta) Nombre Fecha

    E-print Network

    Ma, Lena

    Electrónico* Firma UF/IFAS Laboratorio de Servicios Analíticos Laboratorio de Extensión de Análisis de Suelos. No envié efectivo por correo. Publicado noviembre 2013 SL135-Span ANÁLISIS DE SUELO PARA PRODUCTORES Llenar el mismo suelo, tipo, aspecto, o uso histórico. Muestree las áreas problemáticas por separado, si es

  16. Instituto Superior Tecnico Departamento de Matematica

    E-print Network

    Cannas da Silva, Ana

    A -- TESTE 3 -- 25/NOVEMBRO/2003 (c) Calcule o volume do paralelepâ??�pedo definido por v 1 , v 2 e v 3 . Resoluâ?ºcâ?ao: O volume do paralelepâ??�pedo definido por v 1 , v 2 e v 3 â??e igual ao mâ??odulo do determinante

  17. Instituto Superior Tecnico Departamento de Matematica

    E-print Network

    Cannas da Silva, Ana

    LINEAR A -- TESTE 3 -- 25/NOVEMBRO/2003 (c) Calcule o volume do paralelepâ??�pedo definido por v 1 , v 2 e v 3 . Resoluâ?ºcâ?ao: O volume do paralelepâ??�pedo definido por v 1 , v 2 e v 3 â??e igual ao mâ??odulo do

  18. DERECHOS DEL EMPLEADO LEy PARA LA PROtECCin

    E-print Network

    Rock, Chris

    DERECHOS DEL EMPLEADO LEy PARA LA PROtECCión DEL EMPLEADO COntRA LA PRuEbA DEL POLígRAfO PROHIBICIONES ExENCIONES DERECHOS DE LOS ExAMINADOS CUMPLIMIENTO Generalmente se le prohíbe al empleador que le aspirante a un trabajo por haberse negado a someterse a la prueba o por haberse acogido a otros derechos

  19. DECLARACIN DE DERECHOS DE

    E-print Network

    DECLARACIÓN DE DERECHOS DE LOS PROPIETARIOS EN TEXAS PREPARADO POR LA PROCURADURÍA GENERAL DE TEXAS, usted tiene derecho a una audiencia ante un panel asignado por la corte que incluye a tres comisionados compensación, si es necesaria, a la que tiene derecho usted poruna disminución en elvalorde supropiedad

  20. 78 FR 37205 - Freshwater Crawfish Tail Meat From the People's Republic of China: Rescission of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-06-20

    ... Republic of China: Initiation of Antidumping Duty New Shipper Review, 78 FR 24723 (April 26, 2013... the POR.\\2\\ \\2\\ See Antidumping Duties; Countervailing Duties; Final Rule, 62 FR 27296, 27319 (May 19... case was made long after the end of the POR.\\5\\ \\3\\ See Final Rule, 62 FR at 27319-20 (``The...

  1. Estudio Nacional de Exámenes de Pulmón: preguntas y respuestas

    Cancer.gov

    El Estudio Nacional de Exámenes de Pulmón (National Lung Screening Trial, NLST) es un estudio de exámenes selectivos de detección de cáncer de pulmón patrocinado por el Instituto Nacional del Cáncer (NCI) y llevado a cabo por la American College of Radiol

  2. Se calcula que los costos del cáncer ascenderán po

    Cancer.gov

    Con base en el crecimiento y en el avance de edad de la población de EE. UU., se proyecta que los gastos médicos por cáncer en el año 2020 ascenderán por lo menos a $158.000 millones (en dólares de 2010); lo que significa un aumento de 27% sobre 2010. Si

  3. Abigael Bohórquez: Picardía, escarnio y gimnasio verbal

    E-print Network

    Salcedo, Hugo

    1999-04-01

    , particularmente de los inhóspitos pueblos mexicanos del norte, y la preferencia por temas sexuales de gran voltaje (pedofilia, crimen pasional, zoofilia, entre otros), invitan a la lectura y montaje de su obra. Por ello, y con el interés en el conocimiento y...

  4. UNA HISTORIA DE LA CIENCIA

    E-print Network

    Rosas-Ortiz, Jose Oscar

    : a life in science), en coautoría con Mary Gribbin3 . Los trabajos de Gribbin se caracterizan por la archivista, y Johannes Kepler, un brillante matemático y heredero fi- nal de la información recopilada por Brahe. Kepler, quien tenía problemas para financiar su investigación (las famosas becas que nos ator

  5. Boletín del Instituto Nacional del Cáncer

    Cancer.gov

    La edición electrónica del Boletín del Instituto Nacional del Cáncer se publicó mensualmente desde septiembre de 2009 hasta enero de 2013, con el objetivo de difundir información sobre la investigación del cáncer, tanto de estudios financiados por el NCI y otras dependencias federales, así como de investigaciones realizadas por instituciones en Estados Unidos y alrededor del mundo.

  6. Superficies y Vaco 16(1), 40-44, marzo de 2003 Sociedad Mexicana de Ciencia de Superficies y de Vaco Tabla 1. Resultados obtenidos de los programas Teor y Chekcell.

    E-print Network

    Meléndez Lira, Miguel Angel

    transporte de vapor en espacio reducido combinada con evaporación libre (CSVT-FE, por sus siglas en ingles CdSxTe1-x utilizando la técnica de sublimación en espacio cerrado (CSS, por sus siglas en ingles ingles) es un método conveniente para el crecimiento de materiales ternarios debido a que es posible

  7. Diagnóstico y estadificación

    Cancer.gov

    El cáncer puede causar muchos síntomas diferentes. El doctor ordenará pruebas para determinar si los síntomas son causados por cáncer o por algún otro problema. Si se diagnostica cáncer, el doctor efectuará algunas pruebas para determinar el estadio. Al conocer el estadio, el doctor podrá hacer un plan de tratamiento y hablar del pronóstico.

  8. Desarrollo y Aplicacin de un ndice de Calidad de Agua para ros en Puerto Rico

    E-print Network

    Gilbes, Fernando

    Desarrollo y Aplicación de un Índice de Calidad de Agua para ríos en Puerto Rico por Francisco J en INGENIERÍA CIVIL UNIVERSIDAD DE PUERTO RICO RECINTO UNIVERSITARIO DE MAYAGÜEZ 2009 Aprobado por trabajo presenta la elaboración de un Índice de Calidad de Agua (ICA) para los ríos en Puerto Rico. Dicho

  9. A N U A R I O C I N V E S T A V 637 SECCINEXTERNADETOXICOLOGA

    E-print Network

    mayor. Esto ha pro- ducido una creciente preocupación social por los posibles efectos sobre la salud estudios para evaluar los riesgos para la salud derivados de la exposición a contaminantes. Por lo tanto ecológicos, con el propósito de estimar la probabilidad (riesgo) de sufrir efectos nocivos para la salud como

  10. Planificacin Anticipada de Cuidados Mdicos del Estado de Utah Formularios e Instrucciones

    E-print Network

    Tipple, Brett

    sobre su atención de salud, en el evento que usted no las pueda hacer o no pueda hablar por sí mismo. Parte II: Le permite dejar sentados por escrito, sus deseos sobre el cuidado de su salud. Parte III: Le su atención de salud. Puede llenar el formulario completamente o solamente parte de el Usted puede

  11. Festival Report: Primer Festival Internacional de Teatro Santa Cruz de la Sierra, Bolivia

    E-print Network

    Muñ oz, Willy O.

    1998-10-01

    esencialmente jugar a ser otro. Cierra el festival el elenco Casateatro, de Santa Cruz, dirigido por Rene Hohenstein, con Los funerales de la Mama Grande, una adaptación del texto de Gabriel García Márquez realizada por Gonzalo de Cordova. El mítico Macondo...

  12. Teledeteccin de las regiones de humedales de la zona oeste de Puerto Rico, cun saludables se encuentran?

    E-print Network

    Gilbes, Fernando

    permanece inundado o saturado por el agua. Es de suma importancia estudiar estos ecosistemas, pues, además profundas (Cordwin et al., 1979). La localización de estos ecosistemas varían, desde la costa hasta las contaminantes (Munyati, 2000). Por la gran aportación de los mismos al ecosistema, es de suma importancia

  13. E1.4 Acreditacin de las titulaciones ltima revisin

    E-print Network

    Verschure, Paul

    las distintas fases del proceso. Se crea un Comité Interno de Acreditación (CIA) formado por, entre además unas minicomisiones de trabajo dentro del mismo CIA que permiten agilizar la tarea, trabajando el aprueba, por parte de la CIA, el Autoinforme y se hace llegar al Equipo de Gobierno del Centro para su

  14. 80 INVESTIGACION Y CIENCIA, febrero, 2010 or los ros espaoles circulan anualmen-

    E-print Network

    García-Berthou, Emili

    calidad y la disponibilidad del agua. ¿Somos conscientes de la impor- tancia de este recurso? En 2008 la preocupación por la calidad de las aguas repre- sadas y por los conflictos entre las entidades depende en buena medida la calidad del agua embalsada. Fue la prime- ra señal de que algo se estaba

  15. Esta es muy bonita. La chica esa, la de vestido rojo, es Mara.

    E-print Network

    Vellend, Mark

    [= `trabaja sin compañía los domingos']; o ¿Por qué compraron aquéllos libros usados? (aquéllos es el sujeto de la oración), frente a ¿Por qué compraron aquellos libros usados? (el sujeto de esta oración no está expreso y aquellos acompaña al sustantivo libros). #12;#12;

  16. Nuestro inters como Facultad de Ciencias de la Salud es formar a la poblacin general y a determinados sectores profesionales para salvar vidas. La formacin se

    E-print Network

    Escolano, Francisco

    de PCR y la importancia del factor tiempo. Analizar correctamente una situación de emergencia y cuando alertar a los dispositivos de emergencias. Saber desarrollar las maniobras de RCP básica en Profesorado: acreditado por la American Hearts Association (AHA) y por la Sociedad Española de Medicina de

  17. Theory and Applications of Categories, Vol. 11, No. 12, 2003, pp. 283--308. RING EPIMORPHISMS AND C(X)

    E-print Network

    Rosebrugh, Robert

    to preserve the relevant structures: POR/N, the category of reduced partially ordered rings; R/N, the category then it is automatically a morphism in POR/N and R/N. (See [Schwartz & Madden (1999), §12] for basic properties of real

  18. Theory and Applications of Categories, Vol. 11, No. 12, 2003, pp. 283308. RING EPIMORPHISMS AND C(X)

    E-print Network

    Rosebrugh, Robert

    to preserve the relevant structures: POR/N, the category of reduced partially ordered rings; R/N, the category then it is automatically a morphism in POR/N and R/N. (See [Schwartz & Madden (1999), §12] for basic properties of real

  19. 25388 Viernes 12 julio 2002 BOE nm. 166 I. Disposiciones generales

    E-print Network

    Escolano, Francisco

    de acceso a la red, a la transmisión de datos por redes de telecomunicaciones, a la realización de denomina «sociedad de la información» viene determinado por la extraordi- naria expansión de las redes de telecomunicaciones y, en especial, de Internet como vehículo de transmisión e intercambio de todo tipo de información

  20. 78 FR 46318 - Multilayered Wood Flooring From the People's Republic of China: Initiation of Antidumping Duty...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2013-07-31

    ...shipper reviews is December 1, 2012, through May 31, 2013. The POR for the...shipper review is December 1, 2012, through June 30, 2013. DATES: Effective...July and the POR is December 1, 2012, through May 31, 2013. [[Page...

  1. Transportes em Revista.com Pas: Portugal

    E-print Network

    Instituto de Sistemas e Robotica

    menos de 25 km por dia por: Carlos Moura Tags: Automóveis Galp Energia Mobilidade el étrica realizado em Portugal que resultou de uma parceria entre a Toyota Caetano Portugal, a Galp Energia o s f o r a m e n t r e g u e s a o s colaboradores da Galp Energia, residentes nos concelhos de

  2. 77 FR 77017 - Certain Orange Juice From Brazil: Notice of Court Decision Not in Harmony With Final Results of...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-12-31

    ...A-351-840] Certain Orange Juice From Brazil: Notice of Court Decision Not in Harmony...order on certain orange juice (OJ) from Brazil covering the period of review (POR...duty administrative review of OJ from Brazil covering the POR of March 1, 2008,...

  3. UNIVERSITE PARIS XI UFR SCIENTIFIQUE D'ORSAY

    E-print Network

    Gil, Ana Arribas

    . Muchas gracias a G´abor Lugosi por haber venido especialmente para esta ocasi´on. J'ai eu la chance de a Sabine, Daniela, H´ector, Cristian y Natalia, Carmen y Roc´io, Sandra, Laura y Suni por haber estado.2.1 The Insertion and Deletion Process . . . . . . . . . . . . . . . . . . 47 2.2.2 The Markov property

  4. Introduc ~ao `a Teoria da Medida e Integral de Lebesgue

    E-print Network

    Cabral, Marco

    Dominada, Fubini, derivada de Radon-Nikod´ym, espa¸co produto. Por isso inclu´imos estes resultados://dsl.org/copyleft/dsl.txt. Recomendamos como leitura complementar o livro do Bartle (Elements of Integration, ver Bibliografia) por ser um Radon-Nikod´ym e Fubini . . . . . . . . . . . . . . . . . . . . . 32 2.6 Outras Constru¸c~oes da

  5. Planificar la transición a la etapa final de la vida (PDQ)—Versión para pacientes

    Cancer.gov

    Resumen de información revisada por expertos sobre la preparación necesaria por parte de los proveedores de atención de la salud, los pacientes y sus familias para la transición a la atención del cáncer avanzado en la etapa final de la vida.

  6. Buenos Aires, Argentina -25 al 29 de Julio de 2005 Explosiones en Ecuaciones Diferenciales

    E-print Network

    Groisman, Pablo

    modelados por medio de ecuaciones diferenciales ordinarias (EDOs) de la forma (0.1) x(t) = b(x(t)), x(0) = x dado por la ecuación diferencial ordinaria de forma tal de poder incluir efectos aleatorios (ruido) que

  7. Edif. Ada Byron Mara de Luna, 1

    E-print Network

    Baldassarri, Sandra

    humanos: se llevará a cabo por medio de un personaje (virtual) con características antropomórficas, dotado de la capacidad de expresarse por medio de la voz, y de manifestar sus emociones a través de las razonamiento, poder tomar decisiones en base a ellas. Para más detalles sobre estas propuestas, o si estáis

  8. Sistemas de ecuaciones lineales Comenzamos este primer tema con un problema de motivacion.

    E-print Network

    Hurtado, Ana

    de ox´igeno y un 1 por ciento de arg´on. Para poder realizar un experimento en el planeta Marte se forma de rect´angulo, que usualmente se delimitan por medio de par´entesis. Si una matriz tiene n filas

  9. UAMcifrasen Universidad Autnoma de Madrid

    E-print Network

    Autonoma de Madrid, Universidad

    Estudiantes #12;4.3. Perfiles de los Estudiantes 4.3.1. Distribución de los estudiantes por sexo y edad Tabla 13. Estudiantes de 1 er y 2º Ciclo distribuidos según titulación y sexo NIVEL TITULACIÓN HOMBRES de 2003) Fuente: SIGMA, a 1 de Septiembre de 2003 #12;Distribución de estudiantes por sexo en 1er y 2

  10. Planificar la transición en la etapa final de la vida (PDQ)—Versión para profesionales de salud

    Cancer.gov

    Resumen de información revisada por expertos sobre la preparación necesaria por parte de los proveedores de atención de la salud, los pacientes y sus familas para la transición a la atención del cáncer avanzado en la etapa final de la vida.

  11. The Role of Overweight and Obesity in In Vitro Fertilization Outcomes of Poor Ovarian Responders

    PubMed Central

    Vural, Fisun; Vural, Birol; Çak?ro?lu, Yi?it

    2015-01-01

    Objective. Obesity is a worldwide concern with detrimental health effects including decreased fecundity. However, obesity's impact on in vitro fertilization (IVF) is inconclusive and there is little data concerning poor ovarian responders (POR). This study explored the effects of overweight and obesity on IVF outcomes of POR. Design. We retrospectively evaluated 188 POR undergoing IVF cycles. Methods. Patients were categorized into three groups. Group 1 was normal weight POR (18.5–24.9?kg/m2, n = 96); Group 2 was overweight POR (25.0–29.9?kg/m2, n = 52); and Group 3 was obese POR (?30.0?kg/m2, n = 40). Main measured outcomes included IVF outcomes. Results. The oocyte maturity, total gonadotropin dose-duration, and cycle cancellation rates were similar. Obese women had significantly decreased LH levels. LH < 4?mIU/mL had a sensitivity (62%) and a specificity (86%) for IVF failure (AUC: 0.71). Fertilization rates of obese subjects were significantly lower than normal and overweight subjects (p = 0.04). Obese women's clinical pregnancy rates were significantly lower (15%) than normal weight women (33.3%, p = 0.01). Conclusions. Despite similar counts of recruited mature oocytes, obese POR women had decreased fertilization and clinical pregnancy rates. Obesity rather than overweight significantly decreased IVF outcomes in POR. PMID:26106614

  12. El cuento del cáncer de cérvix podría tener un final feliz

    Cancer.gov

    El cuento del cáncer de cérvix podría tener un final feliz Por el Instituto Nacional del Cáncer El cáncer de cérvix fue en un tiempo la causa principal de muerte por cáncer entre mujeres estadounidenses. Pero, en los últimos 50 años, el número de

  13. Un estudio de los NIH indica que el consumo regular de aspirina podría reducir el riesgo de cáncer d

    Cancer.gov

    Las mujeres que toman aspirina diariamente podrían reducir el riesgo de cáncer de ovario en 20 por ciento, de acuerdo con un estudio realizado por científicos del Instituto Nacional del Cáncer (NCI), el cual forma parte de los Institutos Nacionales de la

  14. 2 Doa Ana Community College to the Community College

    E-print Network

    Johnson, Eric E.

    inglés, se ofrecen cursos completamente gratuitos de inglés como segundo idioma por medio del programa de trabajando para eliminar toda barrera en la educación. Aunque casi todos los cursos por crédito se dictan en Educación Básica para Adultos. Si desea información sobre estos cursos, comuníquese al teléfono 527

  15. O R E G O N Informe Final Sobre el Estudio de las

    E-print Network

    WASHINGTON O R E G O N IDAHO HANFORD Resumen del Informe Final Sobre el Estudio de las Enfermedades de la Tiroides en Hanford Acerca del Estudio Sobre las Enfermedades de la Tiroides en Hanford. (HTDS por su sigla en inglés) El estudio sobre las enfermedades de la tiroides en Hanford (HTDS por su sigla

  16. ME414:ESTATISTICA PARA EXPERIMENTALISTAS Prof.: Victor Hugo Lachos Davila -I-2013

    E-print Network

    Lachos, Victor

    por uma barra de a¸co testaram-se n barras, obtendo-se Xn = 20 e o limite superior do intervalo de confian¸ca a 95% igual a 21.7. Sabendo-se que a tens~ao suportada por uma barra de a¸co ´e uma v.a. normal

  17. Problema mundial de la crítica

    E-print Network

    Potenze, Jaime

    1984-04-01

    de Frank Sinatra, que cobró una fortuna en dólares por venir a Buenos Aires . . . pero llenó el Luna Park, que es el estadio más grande de Buenos Aires. Por otro lado (los críticos no cultivamos la antipatía pero a veces no tenemos más remedio que...

  18. UNIVERSIDADE TCNICA DE LISBOA INSTITUTO SUPERIOR TCNICO

    E-print Network

    Sousa, Leonel

    blocos por pesquisa exaustiva. Estes processadores são basea- dos numa arquitectura melhorada da classe de processamento. Contudo, esta arquitectura requer elevada largura de banda no acesso à memória e o débito binário correspondente, propõe-se nesta dissertação uma alteração à arquitectura base por

  19. Avda. Teniente Flomesta, 5. Edif. Convalecencia. 30003 Murcia T. 868 883 000 F. 868 883 603 www.um.es

    E-print Network

    Acacio, Manuel

    , dependiente directamente del Rector y destinada a desarrollar las políticas de igualdad en materia de género la igualdad de género que se desarrollen tanto por iniciativa de la propia Universidad como por la de.um.es REGLAMENTO DE LA UNIDAD PARA LA IGUALDAD ENTRE MUJERES Y HOMBRESDELAUNIVERSIDADDEMURCIA (APROBADO EN CONSEJO

  20. BOE nm. 71 Viernes 23 marzo 2007 12611 I. Disposiciones generales

    E-print Network

    Fitze, Patrick

    ORGÁNICA 3/2007, de 22 de marzo, para la igualdad efectiva de mujeres y hombres. JUAN CARLOS I REY DE Constitución española proclama el derecho a la igualdad y a la no discriminación por razón de sexo. Por su que la igualdad del individuo y de los grupos en que se integra sean reales y efectivas. La igualdad

  1. 06seleccin de contenidos DES-IGUALES

    E-print Network

    Fernández Pascual, Ricardo

    06selección de contenidos IGUALDAD DES-IGUALES Laura Arroyo LA RESPUESTA PATRIARCAL A LA CRISIS: UN NUEVO TIMO A LAS MUJERES Laura Nuño DESIGUALDADES POR GÉNERO Y EDAD. LA DOBLE DESIGUALDAD DE LAS MUJERES. Universidad Rey Juan Carlos. Desigualdades por género y edad. La doble desigualdad de las mujeres jóvenes

  2. Reduction in hepatic drug metabolizing CYP3A4 activities caused by P450 oxidoreductase mutations identified in patients with disordered steroid metabolism

    SciTech Connect

    Flueck, Christa E.; Mullis, Primus E.; Pandey, Amit V.

    2010-10-08

    Research highlights: {yields} Cytochrome P450 3A4 (CYP3A4), metabolizes 50% of drugs in clinical use and requires NADPH-P450 reductase (POR). {yields} Mutations in human POR cause congenital adrenal hyperplasia from diminished activities of steroid metabolizing P450s. {yields} We are reporting that mutations in POR may reduce CYP3A4 activity. {yields} POR mutants Y181D, A457H, Y459H, V492E and R616X lost 99%, while A287P, C569Y and V608F lost 60-85% CYP3A4 activity. {yields} Reduction of CYP3A4 activity may cause increased risk of drug toxicities/adverse drug reactions in patients with POR mutations. -- Abstract: Cytochrome P450 3A4 (CYP3A4), the major P450 present in human liver metabolizes approximately half the drugs in clinical use and requires electrons supplied from NADPH through NADPH-P450 reductase (POR, CPR). Mutations in human POR cause a rare form of congenital adrenal hyperplasia from diminished activities of steroid metabolizing P450s. In this study we examined the effect of mutations in POR on CYP3A4 activity. We used purified preparations of wild type and mutant human POR and in vitro reconstitution with purified CYP3A4 to perform kinetic studies. We are reporting that mutations in POR identified in patients with disordered steroidogenesis/Antley-Bixler syndrome (ABS) may reduce CYP3A4 activity, potentially affecting drug metabolism in individuals carrying mutant POR alleles. POR mutants Y181D, A457H, Y459H, V492E and R616X had more than 99% loss of CYP3A4 activity, while POR mutations A287P, C569Y and V608F lost 60-85% activity. Loss of CYP3A4 activity may result in increased risk of drug toxicities and adverse drug reactions in patients with POR mutations.

  3. Estimación de pequeñas perturbaciones en satélites geocéntricos como un problema inverso

    NASA Astrophysics Data System (ADS)

    Zadunaisky, P. E.

    El movimiento geocéntrico de un satélite artificial es simulado automáticamente por un sistema de ecuaciones diferenciales de segundo orden que incluyen dos funciones perturbadoras. La primera representa el segundo término del potencial gravitatorio de la Tierra y la segunda corresponde al frenado atmosférico. Asumiendo como conocidas, por hipótesis o por mediciones, la posición y velocidad del satélite en instantes sucesivos se estiman las perturbaciones a intervalos sucesivos por un método determinístico. Para ilustrar el método y comprobar la precisión de nuestros resultados hemos simulado dos ejemplos incluyendo valores conocidos de las perturbaciones que luego comparamos con los resultados de nuestro método. El método puede aplicarse por ejemplo para el intervalo de una revolución satelital lo cual permitiría el diseño inmediato de una maniobra correctiva de la órbita satelital.

  4. Deletion of P399{sub E}401 in NADPH cytochrome P450 oxidoreductase results in partial mixed oxidase deficiency

    SciTech Connect

    Flueck, Christa E.; Mallet, Delphine; Hofer, Gaby; Samara-Boustani, Dinane; Leger, Juliane; Polak, Michel; Morel, Yves; Pandey, Amit V.

    2011-09-09

    Highlights: {yields} Mutations in human POR cause congenital adrenal hyperplasia. {yields} We are reporting a novel 3 amino acid deletion mutation in POR P399{sub E}401del. {yields} POR mutation P399{sub E}401del decreased P450 activities by 60-85%. {yields} Impairment of steroid metabolism may be caused by multiple hits. {yields} Severity of aromatase inhibition is related to degree of in utero virilization. -- Abstract: P450 oxidoreductase (POR) is the electron donor for all microsomal P450s including steroidogenic enzymes CYP17A1, CYP19A1 and CYP21A2. We found a novel POR mutation P399{sub E}401del in two unrelated Turkish patients with 46,XX disorder of sexual development. Recombinant POR proteins were produced in yeast and tested for their ability to support steroid metabolizing P450 activities. In comparison to wild-type POR, the P399{sub E}401del protein was found to decrease catalytic efficiency of 21-hydroxylation of progesterone by 68%, 17{alpha}-hydroxylation of progesterone by 76%, 17,20-lyase action on 17OH-pregnenolone by 69%, aromatization of androstenedione by 85% and cytochrome c reduction activity by 80%. Protein structure analysis of the three amino acid deletion P399{sub E}401 revealed reduced stability and flexibility of the mutant. In conclusion, P399{sub E}401del is a novel mutation in POR that provides valuable genotype-phenotype and structure-function correlation for mutations in a different region of POR compared to previous studies. Characterization of P399{sub E}401del provides further insight into specificity of different P450s for interaction with POR as well as nature of metabolic disruptions caused by more pronounced effect on specific P450s like CYP17A1 and aromatase.

  5. An ortholog of farA of Aspergillus nidulans is implicated in the transcriptional activation of genes involved in fatty acid utilization in the yeast Yarrowia lipolytica

    SciTech Connect

    Poopanitpan, Napapol; Kobayashi, Satoshi; Fukuda, Ryouichi; Horiuchi, Hiroyuki; Ohta, Akinori

    2010-11-26

    Research highlights: {yields} POR1 is a Yarrowia lipolytica ortholog of farA involved in fatty acid response in A. nidulans. {yields} Deletion of POR1 caused growth defects on fatty acids. {yields} {Delta}por1 strain exhibited defects in the induction of genes involved in fatty acid utilization. -- Abstract: The yeast Yarrowia lipolytica effectively utilizes hydrophobic substrates such as fatty acids and n-alkanes. To identify a gene(s) regulating fatty acid utilization in Y. lipolytica, we first studied homologous genes to OAF1 and PIP2 of Saccharomyces cerevisiae, but their disruption did not change growth on oleic acid at all. We next characterized a Y. lipolytica gene, POR1 (primary oleate regulator 1), an ortholog of farA encoding a transcriptional activator that regulates fatty acid utilization in Aspergillus nidulans. The deletion mutant of POR1 was defective in the growth on various fatty acids, but not on glucose, glycerol, or n-hexadecane. It exhibited slight defect on n-decane. The transcriptional induction of genes involved in {beta}-oxidation and peroxisome proliferation by oleate was distinctly diminished in the {Delta}por1 strains. These data suggest that POR1 encodes a transcriptional activator widely regulating fatty acid metabolism in Y. lipolytica.

  6. Transcriptional Regulation of the Human P450 Oxidoreductase Gene: Hormonal Regulation and Influence of Promoter Polymorphisms

    PubMed Central

    Tee, Meng Kian; Huang, Ningwu; Damm, Izabella

    2011-01-01

    P450 oxidoreductase (POR) is the flavoprotein that acts as the obligatory electron donor to all microsomal P450 enzymes, including those involved in hepatic drug metabolism as well as three steroidogenic P450 enzymes. The untranslated first exon of human POR was located recently, permitting analysis of human POR transcription. Expression of deletional mutants containing up to 3193 bp of the human POR promoter in human adrenal NCI-H295A and liver Hep-G2 cells located the proximal promoter at ?325/?1 bp from the untranslated exon. Common human POR polymorphisms at ?208 and ?173 had little influence on transcription, but the polymorphism at ?152 reduced transcription significantly in both cell lines. EMSA and supershift assays identified binding of Smad3/Smad4 between ?249 and ?261 and binding of thyroid hormone receptor-? (TR?) at ?240/?245. Chromatin immunoprecipitation showed that Smad3, Smad4, TR?, TR?, and estrogen receptor-? were bound between ?374 and ?149. Cotransfection of vectors for these transcription factors and POR promoter-reporter constructs into both cell types followed by hormonal treatment showed that T3 exerts major tropic effects via TR?, with TR?, estrogen receptor-?, Smad3, and Smad4 exerting lesser, modulatory effects. T3 also increased POR mRNA in both cell lines. Thyroid hormone also is essential for rat liver POR expression but acts via different transcription factor complexes. These are the first data on human POR gene transcription, establishing roles for TR? and Smad3/4 in its expression and indicating that the common polymorphism at ?152 may play a role in genetic variation in steroid biosynthesis and drug metabolism. PMID:21393444

  7. Prevalence odds ratio or prevalence ratio in the analysis of cross sectional data: what is to be done?

    PubMed Central

    Thompson, M. L.; Myers, J. E.; Kriebel, D.

    1998-01-01

    OBJECTIVES: To review the appropriateness of the prevalence odds ratio (POR) and the prevalence ratio (PR) as effect measures in the analysis of cross sectional data and to evaluate different models for the multivariate estimation of the PR. METHODS: A system of linear differential equations corresponding to a dynamic model of a cohort with a chronic disease was developed. At any point in time, a cross sectional analysis of the people then in the cohort provided a prevalence based measure of the effect of exposure on disease. This formed the basis for exploring the relations between the POR, the PR, and the incidence rate ratio (IRR). Examples illustrate relations for various IRRs, prevalences, and differential exodus rates. Multivariate point and interval estimation of the PR by logistic regression is illustrated and compared with the results from proportional hazards regression (PH) and generalised linear modelling (GLM). RESULTS: The POR is difficult to interpret without making restrictive assumptions and the POR and PR may lead to different conclusions with regard to confounding and effect modification. The PR is always conservative relative to the IRR and, if PR > 1, the POR is always > PR. In a fixed cohort and with an adverse exposure, the POR is always > or = IRR, but in a dynamic cohort with sufficient underlying follow up the POR may overestimate or underestimate the IRR, depending on the duration of follow up. Logistic regression models provide point and interval estimates of the PR (and POR) but may be intractable in the presence of many covariates. Proportional hazards and generalised linear models provide statistical methods directed specifically at the PR, but the interval estimation in the case of PH is conservative and the GLM procedure may require constrained estimation. CONCLUSIONS: The PR is conservative, consistent, and interpretable relative to the IRR and should be used in preference to the POR. Multivariate estimation of the PR should be executed by means of generalised linear models or, conservatively, by proportional hazards regression.   PMID:9624282

  8. Moléculas orgánicas obtenidas en simulaciones experimentales del medio interestelar.

    NASA Astrophysics Data System (ADS)

    Muñoz-Caro, Guillermo Manuel

    Las nubes moleculares son regiones de formación de estrellas, con temperaturas cinéticas entre 10-50 K y densidades de 103-106 átomos cm-3. Su materia está formada por gas y polvo interestelar. Estas partículas de polvo están cubiertas por una fina capa de hielo, de unos 0.01 ?m, que contiene H2O y a menudo CO, CO2, CH3OH y NH3. El hielo es presumiblemente irradiado por fotones ultravioleta y rayos cósmicos en las zonas poco profundas de las nubes moleculares y las regiones circunestelares. En un sistema de vacío, P ˜ 10-7 mbar, simulamos la deposición de hielo a partir de 10 K y la irradiación ultravioleta por medio de una lámpara de descarga de hidrógeno activada con microondas. La evolución del hielo se observa por medio de un espectrómetro infrarrojo. De este modo es posible determinar la composición del hielo observado en el medio interestelar y predecir la presencia de moléculas aún no detectadas en el espacio, que han sido producto del procesamiento del hielo en nuestros experimentos. También es posible calentar el sistema hasta temperatura ambiente para sublimar el hielo depositado. Cuando el hielo ha sido previamente irradiado, se observa un residuo compuesto por moléculas orgánicas complejas, algunas prebióticas, como varios ácidos carboxílicos, aminas, amidas, ésteres y en menor proporción moléculas heterocíclicas y aminoácidos. Algunas de estas moléculas podrían detectarse en estado gaseoso por medio de observaciones milimétricas y de radio. También podrían estar presentes en el polvo cometario, cuyo análisis químico está planeado por las misiones Stardust y Rosetta. Mientras tanto, nuestro grupo está llevando a cabo el análisis de partículas de polvo interplanetario (IDPs), algunas de las cuales pueden ser de origen cometario. Al igual que ocurre con los productos obtenidos por irradiación del hielo en nuestros experimentos, algunas IDPs son ricas en material orgánico que contiene oxígeno.

  9. En sus propias palabras: Víctor Hugo Rascón Banda

    E-print Network

    Day, Stuart A.

    2005-01-01

    ese nom bre, condenándome a la escritura. Mi madre siempre tuvo esas inclinaciones artísticas y alentó mi vicio por la lectura y mi vicio por la escritura. Desde niños, en la escuela prima ria, el sistema educativo de Chihuahua era tan avanzado... apellido Banda es por el lado materno. Mi abuelo era enemigo de los Rascón. Los Rascón eran porfiristas e incluso Benito Juárez los autorizó a usar su propia moneda -las monedas que usábamos en la familia en el siglo diecinueve-. Entonces, Porfirio...

  10. Correspondencias estructurales y semánticas entre El gesticulador y Corona de sombra

    E-print Network

    Scarano, Laura Rosana

    1988-10-01

    aclamado por la multitud y su nombre despierta fatales resonancias en Carlota. Ambos personajes son hom- 32 LATEN AMERICAN THEATRE REVIEW bres de provincia y solamente se corporizan en el escenario a través de otro personaje, en ambos casos un profesor... de historia de México, César Rubio y Erasmo Ramírez. En el primer caso por el recurso de sustitución de identidad apoyado en el nombre homónimo, en el segun do caso por los rasgos faciales de la máscara de indio zapoteca que lleva Erasmo, la...

  11. Understanding Forage Intake in Range Animals (Spanish) 

    E-print Network

    Lyons, Robert K.; Machen, Richard V.; Forbes, T. D. A.

    2001-09-10

    /ha puede esperarse una reducci?n en el consumo de un 15%. Los estudios var?an de manera muy amplia en cuanto a la disponibilidad de forraje disponible que limita el consumo de forraje. Por ejemplo, los valores limites reportados van desde 120 hasta 5000kg.../ha. Las diferencias en estos valores est?n relacionadas al tipo de forraje y tipo de animales en estudio. Por lo tanto, la cantidad de forraje por si sola tiene poca utilidad en estimar el impacto de el forraje disponible sobre el consumo. La asignaci...

  12. On-Site Wastewater Treatment Systems: Constructed Wetlands (Spanish) 

    E-print Network

    Lesikar, Bruce J.; Enciso, Juan

    2002-04-18

    instalarse con una inclinaci?n para ayudar a que las aguas negras fluyan por el sistema. Si el c?sped tiene cuestas en la misma direcci?n que el flujo de las aguas negras, el humedal estar? cerca de la superficie del suelo, y as? aumenta su valor est?tico. El... profundidad. El humedal debe tener suficiente ?rea de corte transversal para que el agua fluya hacia ?l. El agua corre por el humedal pasando de un espacio poroso al siguiente mientras permanece por debajo de la superficie de la grava. Si el ?rea de corte...

  13. Shock Chlorination of Wells (Spanish) 

    E-print Network

    McFarland, Mark L.; Dozier, Monty

    2003-06-11

    para el consumo, y ni las personas ni los animales deben tener un contacto prolongado con la misma. Conduzca el tratamiento cuando los grifos y los inodoros no estar?n en uso por lo menos durante 12 horas, preferiblemente por 24 horas. Si hay un sis... ?olor fresco,? o fragancia a lim?n u otros aditivos porque pueden contener sus- tancias qu?micas no adecuadas para el consumo humano o animal. Proceso de cloraci?n La cantidad de cloro necesaria es determinada por la cantidad de agua estancada en el...

  14. Festival Report: Reseña del VIII Festival Internacional de Teatro

    E-print Network

    Dí az, Né stor Gustavo

    1986-10-01

    escena la vida de Frida Kahlo, la célebre pintora esposa de Diego Rivera, representada por dos actrices que no logran comunicar al público el impacto de la tragedia que vivió la legendaria pintora mexicana, porque Frida Kahlo sólo es conocida por los..., un punto de controversia—recordamos su montaje ritual de Edipo Rey que molestó a muchos y gustó a pocos—por su posición experimental, propia y consecuente de su búsqueda. El viernes se presentó el grupo de México Las dos Fridas, que expone en...

  15. El discurso de la memoria teatral peruana en los noventa

    E-print Network

    Ramos-Garcí a, Luis A.

    2000-10-01

    ; también en 1997, Minnesota y el Instituto Nacional de Cultura ofrecieron su auspicio a un programa de ayuda editorial y financiera a Textos de teatro peruano (Lima, 1998), fundándose ese mismo año la Hugo Salazar del Alcázar Peruvian Theater Video... de Oca; y las cuatro ediciones de Textos de teatro peruano (1992-98), dirigidos por Hugo Salazar, Javier Monroy y Mary Soto, y auspiciados los dos últimos números por el Instituto Nacional de Cultura y el de 1998 por la Universidad de Minnesota...

  16. Festival Report: II Festival Iberoamericano de Teatro de Cádiz

    E-print Network

    Reverte Bernal, Concepció n

    1988-10-01

    , un papel central al baile, que sirve de telón de fondo. El grupo no defraudó con su actuación. CUBA: La Compañía Ibarra-Veloz constituida por los actores Mirtha Ibarra y Ramón Veloz, dirigidos por Miriam Lezcano Brito, dio a conocer Weekend en... sociedad contemporánea afectada por la crisis general de valores. La actuación de la pareja Ibarra-Veloz fue excelente, pero, a mi juicio, restó valor a la obra el FAIMOKK 113 strip-tease en público de la protagonista, innecesario en un trabajo de...

  17. Con Eduardo Pavlovsky, cinco años después

    E-print Network

    Giella, Miguel Á ngel

    1988-10-01

    . Bartis es uno de los grandes actores argentinos y un director de raro talento, que dirigió Telaraña, en el '85. Además, están Elvira Oneto y Susi Evans. Susi me acompaña en Potestad. En realidad estamos trabajando, investigando textos que yo he... julio: Camaralenta, en inglés por el conjunto de Los Angeles, Pablo, también en inglés, y yo, en castellano, con Postestad. Este es más o menos el panorama, que por otro lado, es lo que quiero hacer. Por eso tengo ese grupo humano con el que trabajo...

  18. Como Lo Hago Yo: Anomalías del Tubo Neural en Guatemala — Mielomeningocele Unidad de Espina Bífida e Hidrocefalia

    PubMed Central

    Manucci, Graciela; von Quednow, Enzo

    2014-01-01

    En Guatemala nacen por añ 786 niños con defectos de tubo neural. Operamos 65 a 70 niños con mielomenigocele por año. Tenemos equipo multidisciplinario. Recomendamos parto por cesárea. Infección antes de la cirugía es un problema mayor. Derivación tardía es un problema. Disecamos la plaqueta con la técnica clásica. Suturamos la plaqueta para restituir la forma de la médula. Corpectomía en casos de cifósis. Hidrocefalía: Operamos el 80% de los niños. Chiari II: Operamos basados en los síntomas, primero nos aseguramos que la válvula funciona bien. Médula anclada: Operamos basados en los síntomas. PMID:24791216

  19. Vigencia de la realidad venezolana en el teatro de Cesar Rengifo.

    E-print Network

    Suá rez Radillo, Carlos M.

    1972-04-01

    aceite. La mayoría de los ojos y de las mentes se hallaban encandilados por el brillo de la riqueza presentida, que casi se dejaba tocar por los ávidos dedos extendidos. La alucinación de El Dorado volvió a recorrer llanuras y montañas, costas... agotado el aceite no quedó sino la frustración de un esplendor que no llegó nunca. Lagartos y sabandijas habitan ahora el monte que ha surgido sobre ellas; y, por las noches, únicamente los cocuyos abren sus luces fosforadas sobre el hierro mohoso de...

  20. La teatralización de la violencia y la complicidad del espectáculo en Información para extranjeros de Griselda Gambaro

    E-print Network

    Corté s, Jason

    2001-10-01

    . El espectador que se decide por la no participación elude su responsabilidad por el Otro y se somete a un proceso de aceptación, y por ende, de connivencia. Esta ausencia de responsabilidad implica a la vez una pérdida de identidad. En su libro... satisfacción personal al hacerlo.5 La primera articulación nos remite a una de las instancias más lamentables en la historia del mundo occidental: el holocausto judío. En su libro Eichmann in Jerusalem: A Report on the Banality of Evil (1977), Hannah Arendt...