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Sample records for probing seed black

  1. Songlines from Direct Collapse Seed Black Holes

    NASA Astrophysics Data System (ADS)

    Aykutalp, Aycin; Wise, John; Spaans, Marco; Meijerink, Rowin

    2015-01-01

    In the last decade, the growth of supermassive black holes (SMBHs) has been intricately linked to galaxy formation and evolution, and is a key ingredient in the assembly of galaxies. Observations of SMBHs with masses of 109 solar at high redshifts (z~7) poses challenges to the theory of seed black hole formation and their growth in young galaxies. Fundamental to understanding their existence within the first billion years after the Big Bang, is the identification of their formation processes, growth rate and evolution through cosmic time. We perform cosmological hydrodynamic simulations following the growth of direct collapse seed black holes (DCBH) including X-ray irradiation from the central black hole, stellar feedback both from metal-free and metal-rich stars and H2 self-shielding. These simulations demonstrate that X-ray irradiation from the central black hole regulates its growth and influence the formation of stellar population in the host halo. In particular, X-ray radiation enhances H2 formation in metal-free gas and initially induces the star formation in the halo. However, in the long term, X-ray irradiation from the accreting seed DCBH stifles the initial growth relative to the Eddington rate argument. This further complicates the explanation for the existence of SMBHs in the early universe.

  2. SUPERMASSIVE SEEDS FOR SUPERMASSIVE BLACK HOLES

    SciTech Connect

    Johnson, Jarrett L.; Whalen, Daniel J.; Li Hui; Holz, Daniel E.

    2013-07-10

    Recent observations of quasars powered by supermassive black holes (SMBHs) out to z {approx}> 7 constrain both the initial seed masses and the growth of the most massive black holes (BHs) in the early universe. Here we elucidate the implications of the radiative feedback from early generations of stars and from BH accretion for popular models for the formation and growth of seed BHs. We show that by properly accounting for (1) the limited role of mergers in growing seed BHs as inferred from cosmological simulations of early star formation and radiative feedback, (2) the sub-Eddington accretion rates of BHs expected at the earliest times, and (3) the large radiative efficiencies {epsilon} of the most massive BHs inferred from observations of active galactic nuclei at high redshift ({epsilon} {approx}> 0.1), we are led to the conclusion that the initial BH seeds may have been as massive as {approx}> 10{sup 5} M{sub Sun }. This presents a strong challenge to the Population III seed model, which calls for seed masses of {approx}100 M{sub Sun} and, even with constant Eddington-limited accretion, requires {epsilon} {approx}< 0.09 to explain the highest-z SMBHs in today's standard {Lambda}CDM cosmological model. It is, however, consistent with the prediction of the direct collapse scenario of SMBH seed formation, in which a supermassive primordial star forms in a region of the universe with a high molecule-dissociating background radiation field, and collapses directly into a 10{sup 4}-10{sup 6} M{sub Sun} seed BH. These results corroborate recent cosmological simulations and observational campaigns which suggest that these massive BHs were the seeds of a large fraction of the SMBHs residing in the centers of galaxies today.

  3. Formative Assessment Probes: Seeds in a Bag

    ERIC Educational Resources Information Center

    Keeley, Page

    2014-01-01

    Many young children come to school with prior experiences planting seeds in a garden or in a pot, watering them, and seeing them grow. These early scientific investigations are designed to help children understand that seeds need water, something to grow in (such as soil), and the right temperature to sprout--if these conditions are met, a seed…

  4. Seed for general rotating non-extremal black holes of {N}= 8 supergravity

    NASA Astrophysics Data System (ADS)

    Chow, David D. K.; Compère, Geoffrey

    2014-01-01

    We describe the most general asymptotically flat, stationary, non-extremal, dyonic black hole of the four-dimensional {N}= 2 supergravity coupled to three vector multiplets that describes the low-energy regime of the STU model. Under U-dualities, this can be used as a seed to generate all single-centered stationary black holes of {N}= 8 supergravity. The independent conserved charges are the mass, angular momentum, four electric charges and four magnetic charges; an independent NUT charge can also be added. Several aspects of the black hole are presented, including thermodynamics, the BPS limit, the near-horizon limit in the extremal fast and slow rotating cases, properties of black hole horizons, the existence of Killing tensors and the separability of probe scalars.

  5. Rotating black hole thermodynamics with a particle probe

    SciTech Connect

    Gwak, Bogeun; Lee, Bum-Hoon

    2011-10-15

    The thermodynamics of Myers-Perry black holes in general dimensions are studied using a particle probe. When undergoing particle absorption, the changes of the entropy and irreducible mass are shown to be dependent on the particle radial momentum. The black hole thermodynamic behaviors are dependent on dimensionality for specific rotations. For a 4-dimensional Kerr black hole, its black hole properties are maintained for any particle absorption. 5-dimensional black holes can avoid a naked ring singularity by absorbing a particle in specific momenta ranges. Black holes over 6 dimensions become ultraspinning black holes through a specific form of particle absorption. The microscopical changes are interpreted in limited cases of Myers-Perry black holes using Kerr/CFT correspondence. We systematically describe the black hole properties changed by particle absorption in all dimensions.

  6. Black holes are neither particle accelerators nor dark matter probes.

    PubMed

    McWilliams, Sean T

    2013-01-01

    It has been suggested that maximally spinning black holes can serve as particle accelerators, reaching arbitrarily high center-of-mass energies. Despite several objections regarding the practical achievability of such high energies, and demonstrations past and present that such large energies could never reach a distant observer, interest in this problem has remained substantial. We show that, unfortunately, a maximally spinning black hole can never serve as a probe of high energy collisions, even in principle and despite the correctness of the original diverging energy calculation. Black holes can indeed facilitate dark matter annihilation, but the most energetic photons can carry little more than the rest energy of the dark matter particles to a distant observer, and those photons are actually generated relatively far from the black hole where relativistic effects are negligible. Therefore, any strong gravitational potential could probe dark matter equally well, and an appeal to black holes for facilitating such collisions is unnecessary. PMID:23383773

  7. Small Seed Black Hole Growth in Various Accretion Regimes

    NASA Astrophysics Data System (ADS)

    Gerling-Dunsmore, Hannalore J.; Hopkins, Philip F.

    2016-03-01

    Observational evidence indicates a population of super massive black holes (SMBHs) (~109 -1010M⊙) formed within 1 Gyr after the Big Bang. One proposed means of SMBH formation is accretion onto small seed black holes (BHs) (~ 100M⊙). However, the existence of SMBHs within 1 Gyr requires rapid growth, but conventional models of accretion fail to grow the seed BHs quickly enough. Super Eddington accretion (Ṁ >ṀEddington) may aid in improving growth efficiency. We study small seed BH growth via accretion in 3D, using the magneto-hydrodynamics+gravity code GIZMO. In particular, we consider a BH in a high density turbulent star-forming cloud, and ask whether or not the BH can capture sufficient gas to grow rapidly. We consider both Eddington-limited and super Eddington regimes, and resolve physics on scales from 0.1 pc to 1 kpc while including detailed models for stellar feedback physics, including stellar winds, supernovae, radiation pressure, and photo-ionization. We present results on the viability of different small seed BHs growing into SMBH candidates.

  8. Probing the puncture for black hole simulations

    SciTech Connect

    Brown, J. David

    2009-10-15

    With the puncture method for black hole simulations, the second infinity of a wormhole geometry is compactified to a single 'puncture point' on the computational grid. The region surrounding the puncture quickly evolves to a trumpet geometry. The computational grid covers only a portion of the trumpet throat. It ends at a boundary whose location depends on resolution. This raises the possibility that perturbations in the trumpet geometry could propagate down the trumpet throat, reflect from the puncture boundary, and return to the black hole exterior with a resolution-dependent time delay. Such pathological behavior is not observed. This is explained by the observation that some perturbative modes propagate in the conformal geometry, others propagate in the physical geometry. The puncture boundary exists only in the physical geometry. The modes that propagate in the physical geometry are always directed away from the computational domain at the puncture boundary. The finite difference stencils ensure that these modes are advected through the boundary with no coupling to the modes that propagate in the conformal geometry. These results are supported by numerical experiments with a code that evolves spherically symmetric gravitational fields with standard Cartesian finite difference stencils. The code uses the Baumgarte-Shapiro-Shibata-Nakamura formulation of Einstein's equations with 1+log slicing and gamma-driver shift conditions.

  9. Transmission pump-probe spectroscopy on multilayer black phosphorus

    NASA Astrophysics Data System (ADS)

    Suess, Ryan J.; Jadidi, Mohammad M.; Murphy, Thomas E.; Mittendorff, Martin

    Black phosphorus is a two-dimensional material that has recently attracted interest due to its high mobility and direct bandgap. In this work we examine the pump-induced change in optical transmission of mechanically exfoliated black phosphorus flakes using a two-color optical pump-probe measurement. The time-resolved data reveal a fast pump-induced transparency accompanied by a slower absorption that we attribute to Pauli blocking and free-carrier absorption, respectively. Polarization studies show that these effects are also highly anisotropic - underscoring the importance of crystal orientation in the design of optical devices based on this material. Ongoing work suggests that exploring the carrier density dependence of the pump-probe signals, which can be accessed experimentally via electrostatic gating, may allow for improved understanding of the optical response and carrier dynamics in the material. Supported by NSF and ONR-MURI.

  10. Initial mass function of intermediate-mass black hole seeds

    NASA Astrophysics Data System (ADS)

    Ferrara, A.; Salvadori, S.; Yue, B.; Schleicher, D.

    2014-09-01

    We study the initial mass function (IMF) and hosting halo properties of intermediate-mass black holes (IMBHs, 104-6 M⊙) formed inside metal-free, UV-illuminated atomic-cooling haloes (virial temperature Tvir ≥ 104 K) either via the direct collapse of the gas or via an intermediate supermassive star (SMS) stage. These IMBHs have been recently advocated as the seeds of the supermassive black holes observed at z ≈ 6. We achieve this goal in three steps: (a) we derive the gas accretion rate for a proto-SMS to undergo General Relativity instability and produce a direct collapse black hole (DCBH) or to enter the zero-age main sequence and later collapse into an IMBH; (b) we use merger-tree simulations to select atomic-cooling haloes in which either a DCBH or SMS can form and grow, accounting for metal enrichment and major mergers that halt the growth of the proto-SMS by gas fragmentation. We derive the properties of the hosting haloes and the mass distribution of black holes at this stage, and dub it the `birth mass function'; (c) we follow the further growth of the DCBH by accreting the leftover gas in the parent halo and compute the final IMBH mass. We consider two extreme cases in which minihaloes (Tvir < 104 K) can (fertile) or cannot (sterile) form stars and pollute their gas leading to a different IMBH IMF. In the (fiducial) fertile case, the IMF is bimodal extending over a broad range of masses, M ≈ (0.5-20) × 105 M⊙, and the DCBH accretion phase lasts from 10 to 100 Myr. If minihaloes are sterile, the IMF spans the narrower mass range M ≈ (1-2.8) × 106 M⊙, and the DCBH accretion phase is more extended (70-120 Myr). We conclude that a good seeding prescription is to populate haloes (a) of mass 7.5 < log (Mh/ M⊙) < 8, (b) in the redshift range 8 < z < 17, (c) with IMBH in the mass range 4.75 < (log M•/ M⊙) < 6.25.

  11. Probing spacetime noncommutative constant via charged astrophysical black hole lensing

    NASA Astrophysics Data System (ADS)

    Ding, Chikun; Jing, Jiliang

    2011-10-01

    We study the influence of the spacetime noncommutative parameter on the strong field gravitational lensing in the noncommutative Reissner-Nordström black-hole spacetime. Supposing that the gravitational field of the supermassive central object of the Galaxy is described by this metric, we estimate the numerical values of the coefficients and observables for strong gravitational lensing. Our results show that with the increase of the parameter sqrt {\\vartheta } , the observables θ ∞ and r m decrease, while s increases. Our results also show that i) if sqrt {\\vartheta } is strong, the observables are close to those of the noncommutative Schwarzschild black hole lensing; ii) if sqrt {\\vartheta } is weak, the observables are close to those of the commutative Reissner-Nordström black hole lensing; iii) the detectable scope of ϑ in a noncommutative Reissner-Nordström black hole lensing is 0.12 ≤ sqrt {\\vartheta } ≤ 0.26 , which is wider than that in a noncommutative Schwarzschild black hole lensing, 0.18 ≤ sqrt {\\vartheta } ≤ 0.26 . This may offer a way to probe the spacetime noncommutative constant ϑ by the astronomical instruments in the future.

  12. A Particle Probing Thermodynamics in Rotating AdS Black Hole

    NASA Astrophysics Data System (ADS)

    Gwak, Bogeun; Lee, Bum-Hoon

    2016-07-01

    We briefly review the thermodynamics of a probe particle absorption to a black hole in this proceeding. The particle energy has a relation to its momenta at the horizon of the black hole. Following this relation, the particle infinitesimally changes the black hole mass and momenta. Under these changes, the changes of properties of the black hole are consistent with the laws of thermodynamics.

  13. On the number density of "direct collapse" black hole seeds

    NASA Astrophysics Data System (ADS)

    Habouzit, Mélanie; Volonteri, Marta; Latif, Muhammad; Dubois, Yohan; Peirani, Sébastien

    2016-08-01

    Supermassive black holes (BHs) reside in the center of most local galaxies, but they also power active galactic nuclei and quasars, detected up to z = 7. These quasars put constraints on early BH growth and the mass of BH seeds. The scenario of "direct collapse" is appealing as it leads to the formation of large mass BH seeds, 10^4-10^6 {M_{⊙}}, which eases explaining how quasars at z = 6 - 7 are powered by BHs with masses >109 M⊙. Direct collapse, however, appears to be rare, as the conditions required by the scenario are that gas is metal-free, the presence of a strong photo-dissociating Lyman-Werner flux, and large inflows of gas at the center of the halo, sustained for 10 - 100 Myr. We performed several cosmological hydrodynamical simulations that cover a large range of box sizes and resolutions, thus allowing us to understand the impact of several physical processes on the distribution of direct collapse BHs. We identify halos where direct collapse can happen, and derive the number density of BHs. We also investigate the discrepancies between hydrodynamical simulations, direct or post-processed, and semi-analytical studies. Under optimistic assumptions, we find that for direct collapse to account for BHs in normal galaxies, the critical Lyman-Werner flux required for direct collapse must be about two orders of magnitude lower than predicted by 3D simulations that include detailed chemical models. However, when supernova feedback is relatively weak, enough direct collapse BHs to explain z = 6 - 7 quasars can be obtained for Lyman-Werner fluxes about one order of magnitude lower than found in 3D simulations.

  14. Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The nature and kinetics of reactions in dry seeds determines how long they survive. We used gas chromatography to assay volatile organic compounds (VOC) emitted from seeds of three unrelated species as a means to non-invasively probe chemical changes during very dry, dry and humid (15, 33 and 75% RH...

  15. Massive primordial black holes from hybrid inflation as dark matter and the seeds of galaxies

    NASA Astrophysics Data System (ADS)

    Clesse, Sébastien; García-Bellido, Juan

    2015-07-01

    In this paper we present a new scenario where massive primordial black holes (PBHs) are produced from the collapse of large curvature perturbations generated during a mild-waterfall phase of hybrid inflation. We determine the values of the inflaton potential parameters leading to a PBH mass spectrum peaking on planetarylike masses at matter-radiation equality and producing abundances comparable to those of dark matter today, while the matter power spectrum on scales probed by cosmic microwave background (CMB) anisotropies agrees with Planck data. These PBHs could have acquired large stellar masses today, via merging, and the model passes both the constraints from CMB distortions and microlensing. This scenario is supported by Chandra observations of numerous BH candidates in the central region of Andromeda. Moreover, the tail of the PBH mass distribution could be responsible for the seeds of supermassive black holes at the center of galaxies, as well as for ultraluminous x-ray sources. We find that our effective hybrid potential can originate e.g. from D-term inflation with a Fayet-Iliopoulos term of the order of the Planck scale but sub-Planckian values of the inflaton field. Finally, we discuss the implications of quantum diffusion at the instability point of the potential, able to generate a Swiss-cheese-like structure of the Universe, eventually leading to apparent accelerated cosmic expansion.

  16. Enhancement of nutritionally significant constituents of black currant seeds by chemical elicitor application.

    PubMed

    Flores, Gema; Ruiz del Castillo, María Luisa

    2016-03-01

    Black currant seeds are obtained as a residue during juice production. Black currant seed oil contains high amounts of nutritionally desirable constituents such as γ-linolenic acid (GLA), α-linolenic acid (ALA) and stearidonic acid (SA), as well as certain phenolic acids, which act as natural antioxidants. Fatty acids and phenolic acids of seeds from black currant cultivars after elicitation with methyl jasmonate (MJ) were examined. GLA contents around 25% with respect to total fatty acid content were measured in seeds after pre-harvest treatment of black currants with 0.02mM MJ in 0.05% Tween-20. High GLA samples also exhibited high SA content (higher than 10% with respect to total fatty acid content); however, ALA dropped (from 16% to 10%). High GLA content seeds also showed increased contents of gallic, caffeic, p-coumaric and ferulic acids. In particular, seeds from 0.02mM MJ treated Ben Hope black currants exerted contents of gallic, caffeic, p-coumaric and ferulic acids of 201.4, 125.9, 201.3 and 112.5μgg(-1)vs 124.3, 58.6, 165.4 and 95.8μgg(-1) measured in seeds from untreated Ben Hope black currants. Comparable results were obtained for Ben Alder and Ben Gairn berries. Chemical elicitation with 0.02 MJ is proposed as an industrial practice in such a way that, after consideration of quality issues, it would be obtained high added value black currant seeds. PMID:26471680

  17. The Fossil Record of Black Hole Seeds, with Spatially Resolved Spectroscopy

    NASA Astrophysics Data System (ADS)

    Trump, Jonathan R.; CANDELS, 3D-HST

    2016-01-01

    I will present the first robust measurement of black hole occupation over a wide range of host galaxy mass (8seed formation: while many low-mass galaxies host massive black holes, their black hole occupation is ~10% that of massive galaxies. The measured black hole occupation qualitatively agrees with theoretical models of black hole formation, with massive direct-collapse seeds forming only in massive halos and black hole formation confined to lower-mass Pop III remnants in small halos.

  18. Metabolic Characteristics in Meal of Black Rapeseed and Yellow-Seeded Progeny of Brassica napus-Sinapis alba Hybrids.

    PubMed

    Jiang, Jinjin; Wang, Yue; Xie, Tao; Rong, Hao; Li, Aimin; Fang, Yujie; Wang, Youping

    2015-01-01

    Breeding of yellow-seeded rapeseed (Brassica napus) is preferred over black-seeded rapeseed for the desirable properties of the former. This study evaluated the metabolites and nutritive values of black-seeded rapeseed meal and yellow-seeded meal from the progeny of a B. napus-Sinapis alba hybrid. Yellow-seed meal presented higher protein (35.46% vs. 30.29%), higher sucrose (7.85% vs. 7.29%), less dietary fiber (26.19% vs. 34.63%) and crude fiber (4.56% vs. 8.86%), and less glucosinolates (22.18 vs. 28.19 μmol/g) than black-seeded one. Amounts of ash (3.65% vs. 4.55%), phytic acid (4.98% vs. 5.60%), and total polyphenols (2.67% vs. 2.82%) were decreased slightly in yellow-seeded meal compared with black-seeded meal. Yellow-seeded meal contained more essential amino acids than black-seeded meal. Levels of the mineral elements Fe, Mn, and Zn in yellow-seeded meal were higher than black-seeded meal. By contrast, levels of P, Ca, and Mg were lower in yellow-seeded meal. Moreover, yellow-seeded meal showed lower flavonol (kaempferol, quercetin, isorhamnetin, and their derivatives) content than black-seeded meal. Comparison of metabolites between yellow and black rapeseed confirmed the improved nutritional value of meal from yellow-seeded B. napus, and this would be helpful to the breeding and improvement of rapeseed for animal feeding. PMID:26633322

  19. Differential Scanning Calorimetry as a Tool for Nondestructive Measurements of Seed Deterioration in Lettuce (Lactuca sativa, CV “Black Seeded Simpson”)

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This study was undertaken to determine if changes in lipid phase behavior could be used to detect lost viability in lettuce (Lactuca sativa) seeds. We used seeds from the cultivar ‘Black Seeded Simpson’ that were purchased every 2-3 years since 1989 and stored in resealable plastic bags at constan...

  20. The impact of foliar boron sprays on reproductive biology and seed quality of black gram.

    PubMed

    Pandey, Nalini; Gupta, Bhavana

    2013-01-01

    An experiment was conducted under glass house condition to study the effect of foliar application of boron (B) on reproductive biology and seed quality of black gram (Vigna mungo). Black gram (V. mungo L. var. DPU-88-31) was grown under controlled sand culture condition at deficient and sufficient B levels. After 32 days of sowing B deficient plants were sprayed with three concentrations of B (0.05%, 0.1% and 0.2% borax) at three different stages of reproductive development, i.e. prior to flowering, initiation of bud formation and after bud formation. Deficient B supply decreased the anther and pollen size, pollen tube growth, pollen viability as well as stigmatic receptivity which were increased by foliar B application. Foliar spray at all the three concentrations and at all stages increased the yield parameters like number of pods, pod size and number of seeds formed per plant. Foliar B application also improved the seed yield and seed quality in terms of storage seed proteins (albumin, globulin, glutenin and prolamin) and carbohydrates (sugars and starch) in black gram. The foliar application of B in appropriate doses (particularly 0.1%) after bud formation made quantitative and qualitative improvement in seed yield of black gram by supplementing additional/critical B requirements for reproductive development. PMID:22947393

  1. Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration.

    PubMed

    Mira, Sara; Hill, Lisa M; González-Benito, M Elena; Ibáñez, Miguel Angel; Walters, Christina

    2016-03-01

    The nature and kinetics of reactions in dry seeds determines how long the seeds survive. We used gas chromatography to assay volatile organic compounds (VOCs) emitted from seeds of three unrelated species as a means to non-invasively probe chemical changes during very dry, dry, and humid storage (seeds were dried to 5.5, 33, and 75% relative humidity at room temperature). VOCs emitted from seeds stored in humid conditions reflected fermentation-type reactions, with methanol and ethanol being predominant in Lactuca sativa and Carum carvi, and acetaldehyde and acetone being predominant in Eruca vesicaria. Dried C. carvi seeds continued to emit fermentation-type products, although at slower rates than the seeds stored in humid conditions. In contrast, drying caused a switch in VOC emission in L. sativa and E. vesicaria seeds towards higher emission of pentane and hexanal, molecules considered to be byproducts from the peroxidation of polyunsaturated fatty acids. Longevity correlated best with the rate of fermentation-type reactions and appeared unrelated to the rate of lipid peroxidation. Emission of VOCs decreased when seed species were mixed together, indicating that seeds adsorbed VOCs. Adsorption of VOCs did not appear to damage seeds, as longevity was not affected in seed mixtures. Collectively, the study shows similarity among species in the types of reactions that occur in dry seeds, but high diversity in the substrates, and hence the byproducts, of the reactions. Moreover, the study suggests that the most abundant VOCs arise from degradation of storage reserves within seed cells, and that these reactions and their byproducts are not, in themselves, damaging. PMID:26956506

  2. Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration

    PubMed Central

    Mira, Sara; Hill, Lisa M.; González-Benito, M. Elena; Ibáñez, Miguel Angel; Walters, Christina

    2016-01-01

    The nature and kinetics of reactions in dry seeds determines how long the seeds survive. We used gas chromatography to assay volatile organic compounds (VOCs) emitted from seeds of three unrelated species as a means to non-invasively probe chemical changes during very dry, dry, and humid storage (seeds were dried to 5.5, 33, and 75% relative humidity at room temperature). VOCs emitted from seeds stored in humid conditions reflected fermentation-type reactions, with methanol and ethanol being predominant in Lactuca sativa and Carum carvi, and acetaldehyde and acetone being predominant in Eruca vesicaria. Dried C. carvi seeds continued to emit fermentation-type products, although at slower rates than the seeds stored in humid conditions. In contrast, drying caused a switch in VOC emission in L. sativa and E. vesicaria seeds towards higher emission of pentane and hexanal, molecules considered to be byproducts from the peroxidation of polyunsaturated fatty acids. Longevity correlated best with the rate of fermentation-type reactions and appeared unrelated to the rate of lipid peroxidation. Emission of VOCs decreased when seed species were mixed together, indicating that seeds adsorbed VOCs. Adsorption of VOCs did not appear to damage seeds, as longevity was not affected in seed mixtures. Collectively, the study shows similarity among species in the types of reactions that occur in dry seeds, but high diversity in the substrates, and hence the byproducts, of the reactions. Moreover, the study suggests that the most abundant VOCs arise from degradation of storage reserves within seed cells, and that these reactions and their byproducts are not, in themselves, damaging. PMID:26956506

  3. Observational Constraints on the Nature of the First Supermassive Black Holes Seeds in the Early Universe

    NASA Astrophysics Data System (ADS)

    Treister, Ezequiel; Schawinski, Kevin; Natarajan, Priyamvada; Weigel, Anna

    2015-08-01

    We constrain the total accreted mass density in supermassive black holes at z>6, as inferred from the integrated X-ray emission in a sample of galaxy candidates selected using observed-frame optical and near-IR dropout techniques. Combining galaxy samples acquired in the Hubble Ultra Deep Field with recent deep Hubble observations of the CANDELS fields and Chandra 4 Msec observations we obtain the most restrictive current constraints on total black hole growth in the early Universe, estimating a mass density <1000M⊙Mpc-3. We further carry out a detailed study of all the individually-detected X-ray sources in the Chandra Deep Field South, finding that none of them is a good candidate to be at z>5.These results place interesting constraints on growth by accretion and imply one or more of the following: only ~20% luminous galaxies at this epoch are seeded with BHs - so seeding is inefficient; most black hole growth at early epochs happens in dusty - as yet undetected - host galaxies and/or in less-massive - also as yet undetected - galaxies; therefore a large fraction of the early black hole buildup is heavily obscured or that either most of the growth is due to radiatively inefficient accretion or due to black hole mergers at these early times. Not seeing a signal from growing black holes in high-redshift galaxies suggests that if their black holes are growing they are doing so in a veiled fashion, or they are simply not growing or perhaps most galaxies do not harbor black holes at their centers at all. These possibilities offer novel insights for high redshift seed formation models.Finally, we present the prospects to constrain the nature of the first black hole seeds in the early Universe using existing and planned space-based facilities.

  4. Seed-borne nature of a begomovirus, Mung bean yellow mosaic virus in black gram.

    PubMed

    Kothandaraman, Satya Vijayalakshmi; Devadason, Alice; Ganesan, Malathi Varagur

    2016-02-01

    The yellow mosaic viruses (YMV) infecting legumes are considered to be the most devastating begomoviruses as they incite considerable yield loss. The yellow discoloration of pods and seeds of infected plants and symptom emergence in the very first trifoliate leaf of the plants in the field were suggestive that the virus may be seed borne, which was investigated in the present study. The distribution of the virus in various parts of the seeds of black gram (Vigna mungo L. Hepper) plants naturally infected in the field was determined by polymerase chain reaction (PCR), Southern blot analysis, and sequencing. Nucleotide sequencing of the PCR amplicons from the seed parts from groups of ten seeds revealed the presence of mung bean yellow mosaic virus (MYMV) in the seed coat, cotyledon, and embryonic axes. The presence of virion particles was confirmed through double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunosorbent electron microscopy (ISEM) even in a single whole seed. In confocal microscopy, positive fluorescent signals were obtained using coat protein gene-specific primers in the embryonic axes. However, in the growth tests performed with the same batch of seeds, there was no symptom development in the seedlings though the virus (both DNA A and B components) was detected in 32 % of tested seedlings. In this study, the MYMV was detected in seed coat, cotyledon, and embryo. This study revealed that the MYMV is a seed-borne virus. PMID:26646557

  5. Consumption of seeds of southwestern white pine (Pinus strobiformis) by Black Bear (Ursus americanus)

    USGS Publications Warehouse

    Mattson, David J.; Arundel, Terry A.

    2013-01-01

    We report a discovery of black bears (Ursus americanus) consuming seeds of southwestern white pine (Pinus strobiformis) on north slopes of the San Francisco Peaks near Flagstaff, Arizona, in high-elevation, mixed-species conifer forest. In one instance, a bear had obtained seeds from cones excavated from a larder horde made by a red squirrel (Tamiasciurus hudsonicus). Consumption of seeds of southwestern white pine by bears had not been previously documented. This discovery adds to the number of species of pine used by bears for food as well as the geographic range within which the behavior occurs.

  6. The black hole symphony: probing new physics using gravitational waves.

    PubMed

    Gair, Jonathan R

    2008-12-13

    The next decade will very likely see the birth of a new field of astronomy as we become able to directly detect gravitational waves (GWs) for the first time. The existence of GWs is one of the key predictions of Einstein's theory of general relativity, but they have eluded direct detection for the last century. This will change thanks to a new generation of laser interferometers that are already in operation or which are planned for the near future. GW observations will allow us to probe some of the most exotic and energetic events in the Universe, the mergers of black holes. We will obtain information about the systems to a precision unprecedented in astronomy, and this will revolutionize our understanding of compact astrophysical systems. Moreover, if any of the assumptions of relativity theory are incorrect, this will lead to subtle, but potentially detectable, differences in the emitted GWs. Our observations will thus provide very precise verifications of the theory in an as yet untested regime. In this paper, I will discuss what GW observations could tell us about known and (potentially) unknown physics. PMID:18812300

  7. Probing 2D black phosphorus by quantum capacitance measurements.

    PubMed

    Kuiri, Manabendra; Kumar, Chandan; Chakraborty, Biswanath; Gupta, Satyendra N; Naik, Mit H; Jain, Manish; Sood, A K; Das, Anindya

    2015-12-01

    Two-dimensional materials and their heterostructures have emerged as a new class of materials, not only for fundamental physics but also for electronic and optoelectronic applications. Black phosphorus (BP) is a relatively new addition to this class of materials. Its strong in-plane anisotropy makes BP a unique material for making conceptually new types of electronic devices. However, the global density of states (DOS) of BP in device geometry has not been measured experimentally. Here, we report the quantum capacitance measurements together with the conductance measurements on an hBN-protected few-layer BP (∼six layers) in a dual-gated field effect transistor (FET) geometry. The measured DOS from our quantum capacitance is compared with density functional theory (DFT). Our results reveal that the transport gap for quantum capacitance is smaller than that in conductance measurements due to the presence of localized states near the band edge. The presence of localized states is confirmed by the variable range hopping seen in our temperature dependence conductivity. A large asymmetry is observed between the electron and hole side. This asymmetric nature is attributed to the anisotropic band dispersion of BP. Our measurements establish the uniqueness of quantum capacitance in probing the localized states near the band edge, hitherto not seen in conductance measurements. PMID:26559656

  8. Swift Probes Exotic Object: 'Kicked' Black Hole or Mega Star?

    NASA Video Gallery

    Zoom into Markarian 177 and SDSS1133 and see how they compare with a simulated galaxy collision. When the central black holes in these galaxies combine, a "kick" launches the merged black hole on a...

  9. X-ray 'Echoes' Probe Monster Black Hole's Habitat

    NASA Video Gallery

    Astronomers using data from the European Space Agency's XMM-Newton satellite have found a long-sought X-ray signal from NGC 4151, a galaxy that contains a supermassive black hole. When the black ho...

  10. Sowing Black Hole Seeds: Forming Direct Collapse Black Holes With Realistic Lyman-Werner Radiation Fields in Cosmological Simulations

    NASA Astrophysics Data System (ADS)

    Holley-Bockelmann, Kelly; Dunn, Glenna; Bellovary, Jillian M.; Christensen, Charlotte

    2016-01-01

    Luminous quasars detected at redshifts z > 6 require that the first black holes form early and grow to ~109 solar masses within one Gyr. Our work uses cosmological simulations to study the formation and early growth of direct collapse black holes. In the pre-reionization epoch, molecular hydrogen (H2) causes gas to fragment and form Population III stars, but Lyman-Werner radiation can suppress H2 formation and allow gas to collapse directly into a massive black hole. The critical flux required to inhibit H2 formation, Jcrit, is hotly debated, largely due to the uncertainties in the source radiation spectrum, H2 self-shielding, and collisional dissociation rates. Here, we test the power of the direct collapse model in a non-uniform Lyman-Werner radiation field, using an updated version of the SPH+N-body tree code Gasoline with H2 non-equilibrium abundance tracking, H2 cooling, and a modern SPH implementation. We vary Jcrit from 30 to 104 J21 to study the effect on seed black holes, focusing on black hole formation as a function of environment, halo mass, metallicity, and proximity of the Lyman-Werner source. We discuss the constraints on massive black hole occupation fraction in the quasar epoch, and implications for reionization, high-redshift X-ray background radiation, and gravitational waves.

  11. Wide genetic variation in phenolic compound content of seed coats among black soybean cultivars

    PubMed Central

    Phommalath, Siviengkhek; Teraishi, Masayoshi; Yoshikawa, Takanori; Saito, Hiroki; Tsukiyama, Takuji; Nakazaki, Tetsuya; Tanisaka, Takatoshi; Okumoto, Yutaka

    2014-01-01

    Black soybeans have been used as a food source and also in traditional medicine because their seed coats contain natural phenolic compounds such as proanthocyanidin and anthocyanin. The objective of this research is to reveal the genetic variation in the phenolic compound contents (PCCs) of seed coats in 227 black soybean cultivars, most of which were Japanese landraces and cultivars. Total phenolics were extracted from seed coats using an acidic acetone reagent and the proanthocyanidin content, monomeric anthocyanin content, total flavonoids content, total phenolics content, and radical scavenging activity were measured. The cultivars showed wide genetic variation in PCCs. Each of the contents was highly correlated with one another, and was closely associated with radical scavenging activity. PCCs were also moderately associated by flowering date but not associated by seed weight. Cultivars with purple flowers had a tendency to produce higher PCCs compared with cultivars with white flowers, suggesting that the W1 locus for flower color can affect phenolic compound composition and content. Our results suggest that developing black soybean cultivars with high functional phenolic compounds activity is feasible. PMID:25914597

  12. WE-A-17A-11: Implanted Brachytherapy Seed Movement Due to Transrectal Ultrasound Probe-Induced Prostate Deformation

    SciTech Connect

    Liu, D; Usmani, N; Sloboda, R; Meyer, T; Husain, S; Angyalfi, S; Kay, I

    2014-06-15

    Purpose: To characterize the movement of implanted brachytherapy seeds due to transrectal ultrasound probe-induced prostate deformation and to estimate the effects on prostate dosimetry. Methods: Implanted probe-in and probe-removed seed distributions were reconstructed for 10 patients using C-arm fluoroscopy imaging. The prostate was delineated on ultrasound and registered to the fluoroscopy seeds using a visible subset of seeds and residual needle tracks. A linear tensor and shearing model correlated the seed movement with position. The seed movement model was used to infer the underlying prostate deformation and to simulate the prostate contour without probe compression. Changes in prostate and surrogate urethra dosimetry were calculated. Results: Seed movement patterns reflecting elastic decompression, lateral shearing, and rectal bending were observed. Elastic decompression was characterized by anterior-posterior expansion and superior-inferior and lateral contractions. For lateral shearing, anterior movement up to 6 mm was observed for extraprostatic seeds in the lateral peripheral region. The average intra-prostatic seed movement was 1.3 mm, and the residual after linear modeling was 0.6 mm. Prostate D90 increased by 4 Gy on average (8 Gy max) and was correlated with elastic decompression. For selected patients, lateral shearing resulted in differential change in D90 of 7 Gy between anterior and posterior quadrants, and increase in whole prostate D90 of 4 Gy. Urethra D10 increased by 4 Gy. Conclusion: Seed movement upon probe removal was characterized. The proposed model captured the linear correlation between seed movement and position. Whole prostate dose coverage increased slightly, due to the small but systematic seed movement associated with elastic decompression. Lateral shearing movement increased dose coverage in the anterior-lateral region, at the expense of the posterior-lateral region. The effect on whole prostate D90 was smaller due to the subset

  13. SUPER-CRITICAL GROWTH OF MASSIVE BLACK HOLES FROM STELLAR-MASS SEEDS

    SciTech Connect

    Madau, Piero; Haardt, Francesco; Dotti, Massimo

    2014-04-01

    We consider super-critical accretion with angular momentum onto stellar-mass black holes as a possible mechanism for growing billion-solar-mass black holes from light seeds at early times. We use the radiatively inefficient ''slim disk'' solution—advective, optically thick flows that generalize the standard geometrically thin disk model—to show how mildly super-Eddington intermittent accretion may significantly ease the problem of assembling the first massive black holes when the universe was less than 0.8 Gyr old. Because of the low radiative efficiencies of slim disks around non-rotating as well as rapidly rotating black holes, the mass e-folding timescale in this regime is nearly independent of the spin parameter. The conditions that may lead to super-critical growth in the early universe are briefly discussed.

  14. Probing the Galactic Binary Black Hole Spin with Photon Timing

    NASA Technical Reports Server (NTRS)

    Kazanas, Demosthenes

    2007-01-01

    It is generally considered that the X-ray emission in AGN and Galactic Black Hole Candidates is produced by flares above the surface of a geometrically thin optically thick accretion disk, which extends down to the Innermost Stable Circular Orbit (ISCO) of the black hole. We consider the influence of the black hole geometry on the light curves of these flares. To this end we follow a large number of photon orbits emitted impulsively in a locally isotropic fashion, at any phase of the disk orbit and examine their arrival times at infinity by an observer near the plane of the disk. We find out that the presence of the black hole spin induces a certain delay in the photon arrivals, as prograde photon orbits reach the observer on shorter (on the average) times than the retrograde ones. We form a histogram of the differences in photon time arrivals and we find that it exhibits several well defined peaks depending on the flare position and the black hole spin separated by $\\Delta t \\simeq 30 M$, where M is the black hole mass. The peaks disappear as the spin parameter goes to zero, implying that one could in principle measure the value of the black hole spin with timing measurements of sufficiently high signal to noise ratio.

  15. Probing the Galactic Binary Black Hole Spin with Photon Timing

    NASA Technical Reports Server (NTRS)

    Kazanas, Demos

    2007-01-01

    It is generally considered that the X-ray emission in AGN and Galactic Black Hole Candidates is produced by flares above the surface of a geometrically thin optically thick accretion disk, which extends down to the Innermost Stable Circular Orbit (ISCO) of the black hole. We consider the influence of the black hole geometry on the light curves of these flares. To this end we follow a large number of photon orbits emitted impulsively in a locally isotropic fashion, at any phase of the disk orbit and examine their arrival times at infinity by an observer near the plane of the disk. We find out that the presence of the black hole spin induces a certain delay in the photon arrivals, as prograde photon orbits reach the observer on shorter (on the average) times than the retrograde ones. We form a histogram of the differences in photon time arrivals and we find that it exhibits several well defined peaks depending on the flare position and the black hole spin separated by $\\Delta t\\slmeq 30 M$, where M is the black hole mass. The peaks disappear as the spin parameter goes to zero, implying that one could in principle measure the value of the black hole spin with timing measurements of sufficiently high signal to noise ratio.

  16. Correction: Chemopreventive activity of ellagitannins and their derivatives from black raspberry seeds on HT-29 colon cancer cells.

    PubMed

    Cho, Hyunnho; Jung, Hana; Lee, Heejae; Yi, Hae Chang; Kwak, Ho-Kyung; Hwang, Keum Taek

    2015-08-01

    Correction for 'Chemopreventive activity of ellagitannins and their derivatives from black raspberry seeds on HT-29 colon cancer cells' by Hyunnho Cho et al., Food Funct., 2015, 6, 1675-1683. PMID:26211477

  17. Probing loop quantum gravity with evaporating black holes.

    PubMed

    Barrau, A; Cailleteau, T; Cao, X; Diaz-Polo, J; Grain, J

    2011-12-16

    This Letter aims at showing that the observation of evaporating black holes should allow the usual Hawking behavior to be distinguished from loop quantum gravity (LQG) expectations. We present a full Monte Carlo simulation of the evaporation in LQG and statistical tests that discriminate between competing models. We conclude that contrarily to what was commonly thought, the discreteness of the area in LQG leads to characteristic features that qualify evaporating black holes as objects that could reveal quantum gravity footprints. PMID:22243065

  18. From here to eternity - the secret of Pharaohs: Therapeutic potential of black cumin seeds and beyond

    PubMed Central

    Padhye, Subhash; Banerjee, Sanjeev; Ahmad, Aamir; Mohammad, Ramzi; Sarkar, Fazlul H

    2008-01-01

    Summary Over many centuries humans have been mining the bounties of nature for discovering substances that have been used for the treatment of all human diseases; many such remedies are useful even today as modern day medicine. Emerging evidence also suggests that the search is still continuing for harnessing active compounds from nature in combating human illnesses although pharmaceutical industries are equally active for synthesizing small molecule compounds as novel therapeutics. The lesson learned over many centuries clearly suggests that further sophisticated search for finding compounds from natural resources together with robust characterization and chemical synthesis will lead to the discovery of novel drugs that may have high therapeutic efficacy against all human diseases including cancer. Black cumin seed (Nigella sativa) oil extracts have been used for many centuries for the treatment of many human illnesses, and more recently the active compound found in black seed oil, viz. thymoquinone (TQ) has been tested for its efficacy against several diseases including cancer. However, further research is needed in order to assess the full potential of TQ as a chemopreventive and/or therapeutic agent against cancers. Here, we have summarized what is known regarding the value of black seed oil and its active compound TQ, and how this knowledge will help us to advance further research in this field by creating awareness among scientists and health professionals in order to appreciate the medicinal value of TQ and beyond. PMID:19018291

  19. Anthocyanidins and Flavonols, Major nod Gene Inducers from Seeds of a Black-Seeded Common Bean (Phaseolus vulgaris L.) 1

    PubMed Central

    Hungria, Mariangela; Joseph, Cecillia M.; Phillips, Donald A.

    1991-01-01

    Eleven compounds released from germinating seeds of a black-seeded bean (Phaseolus vulgaris L., cv PI165426CS) induce transcription of nod genes in Rhizobium leguminosarum biovar phaseoli. Aglycones from 10 of those compounds were identified by spectroscopic methods (ultraviolet/visible, proton nuclear magnetic resonance, and mass spectroscopy), and their biological activities were demonstrated by induction of β-galactosidase activity in R. leguminosarum strains containing nodA-lacZ or nodC-lacZ fusions controlled by R. leguminosarum biovar phaseoli nodD genes. By making comparisons with authentic standards, the chemical structures for aglycones from the 10 molecules were confirmed as being anthocyanidins (delphinidin, petunidin, and malvidin) and flavonols (myricetin, quercetin, and kaempferol). All anthocyanidins and flavonols had 3-O-glycosylation and free hydroxyl groups at the 4′, 5, and 7 positions. Hydrolysis experiments showed that the mean concentration required for half-maximum nod gene induction (I50) by the 10 glycosides was about half that of the corresponding aglycones. The mean I50 value for the three anthocyanidins (360 nanomolar) was less (P ≤ 0.05) than that of the three flavonol aglycones (980 nanomolar). Each seed released approximately 2500 nanomoles of anthocyanidin and 450 nanomoles of flavonol nod gene inducers in conjugated forms during the first 6 hours of imbibition. Based on amounts and activities of the compounds released, anthocyanins contributed approximately 10-fold more total nod-inducing activity than flavonol glycosides. These anthocyanidins from bean seeds represent the first nod-inducing compounds identified from that group of flavonoids. PMID:16668462

  20. Phytochemical Contents and Antioxidant and Antiproliferative Activities of Selected Black and White Sesame Seeds

    PubMed Central

    Abbasi, Arshad Mehmood; Zheng, Bisheng

    2016-01-01

    Sesame (Sesamum indicum L.) seeds are popular nutritional food but with limited knowledge about their antioxidant and antiproliferative activities of various varieties. Phytochemical profiles and antioxidant and antiproliferative activities of six varieties of sesame (Sesamum indicum L.) seeds were studied. Fenheizhi3 (black) cultivar exhibited the maximum contents of total phenolics and lignans and values of total oxygen radical absorbance capacity (ORAC) and antiproliferative activity (EC50) against HepG2 cells. Bound ORAC values showed strong associations with bound phenolics contents (r = 0.976, p < 0.01); in bound phenolic extracts, EC50 values showed strong negative associations with phenolic contents (r = −0.869, p < 0.05) and ORAC values (r = −0.918, p < 0.01). Moreover, the contents of free phenolics were higher than that of the bound phenolics, and the three black sesame seeds generally depicted higher total phenolics compared to the three white varieties. The antioxidant (ORAC values) and antiproliferation activities of six sesame seeds were both associated with contents of bound phenolics (r > 0.8, p < 0.05). Interestingly, nonlignan components in bound phenolics contributed to the antioxidant and antiproliferative activities. This study suggested that Fenheizhi3 variety is superior to the other five varieties as antioxidant supplements. PMID:27597975

  1. Phytochemical Contents and Antioxidant and Antiproliferative Activities of Selected Black and White Sesame Seeds.

    PubMed

    Zhou, Lin; Lin, Xiaohui; Abbasi, Arshad Mehmood; Zheng, Bisheng

    2016-01-01

    Sesame (Sesamum indicum L.) seeds are popular nutritional food but with limited knowledge about their antioxidant and antiproliferative activities of various varieties. Phytochemical profiles and antioxidant and antiproliferative activities of six varieties of sesame (Sesamum indicum L.) seeds were studied. Fenheizhi3 (black) cultivar exhibited the maximum contents of total phenolics and lignans and values of total oxygen radical absorbance capacity (ORAC) and antiproliferative activity (EC50) against HepG2 cells. Bound ORAC values showed strong associations with bound phenolics contents (r = 0.976, p < 0.01); in bound phenolic extracts, EC50 values showed strong negative associations with phenolic contents (r = -0.869, p < 0.05) and ORAC values (r = -0.918, p < 0.01). Moreover, the contents of free phenolics were higher than that of the bound phenolics, and the three black sesame seeds generally depicted higher total phenolics compared to the three white varieties. The antioxidant (ORAC values) and antiproliferation activities of six sesame seeds were both associated with contents of bound phenolics (r > 0.8, p < 0.05). Interestingly, nonlignan components in bound phenolics contributed to the antioxidant and antiproliferative activities. This study suggested that Fenheizhi3 variety is superior to the other five varieties as antioxidant supplements. PMID:27597975

  2. THE GROWTH OF THE STELLAR SEEDS OF SUPERMASSIVE BLACK HOLES

    SciTech Connect

    Johnson, Jarrett L.; Li Hui; Whalen, Daniel J.; Fryer, Chris L.

    2012-05-01

    The collapse of baryons into extremely massive stars with masses {approx}>10{sup 4} M{sub Sun} in a small fraction of protogalaxies at z {approx}> 10 is a promising candidate for the origin of supermassive black holes (SMBHs), some of which grow to a billion solar masses by z {approx} 7. We determine the maximum masses such stars can attain by accreting primordial gas. We find that at relatively low accretion rates the strong ionizing radiation of these stars limits their masses to M{sub *} {approx} 10{sup 3} M{sub Sun} ( M-dot{sub acc}/10{sup -3} M{sub Sun} yr{sup -1}){sup 8/7}, where M-dot{sub acc} is the rate at which the star gains mass. However, at the higher central infall rates usually found in numerical simulations of protogalactic collapse ({approx}>0.1 M{sub Sun} yr{sup -1}), the lifetime of the star instead limits its final mass to {approx}10{sup 6} M{sub Sun }. Furthermore, for the spherical accretion rates at which the star can grow, its ionizing radiation is confined deep within the protogalaxy, so the evolution of the star is decoupled from that of its host galaxy. Ly{alpha} emission from the surrounding H II region is trapped in these heavy accretion flows and likely reprocessed into strong Balmer series emission, which may be observable by the James Webb Space Telescope. This, strong He II {lambda}1640, and continuum emission are likely to be the key observational signatures of the progenitors of SMBHs at high redshift.

  3. Protective effect of black raspberry seed containing anthocyanins against oxidative damage to DNA, protein, and lipid.

    PubMed

    Choi, Mi-Hee; Shim, Soon-Mi; Kim, Gun-Hee

    2016-02-01

    This study aimed to determine bioactive components and radical scavenging capacity of black raspberry seed extracts as byproducts obtaining during the juice (FSE) and wine (WSE) making process. Cyanidin-3-O-rutinoside was identified as a major anthocyanin and the total anthocyanin contents of fresh and wine seed were 78.24 and 41.61 mg/100 g of dry weight, respectively. The total phenolic and flavonoid contents of FSE and WSE were 2.31 g gallic acid equivalent (GAE) and 360.95 mg catechin equivalent (CE), and 2.44 g GAE and 379.54 mg CE per 100 g dry weight, respectively. The oxygen radical absorbance capacity (ORAC) values were 1041.9 μM TE/g for FSE and 1060.4 μM TE/g for WSE. Pretreatment of the FSE and WSE inhibited the generation of intracellular reactive oxygen species (ROS), DNA and protein damage induced by hydroxyl radicals, and Fe(3+)/ascorbic acid-induced lipid peroxidation in a dose dependent manner. WSE more effectively protected from oxidative damage than FSE. Results from the current study suggest that black raspberry seeds as byproducts from juice and wine processing could be potential sources for natural antioxidants. PMID:27162401

  4. Poster — Thur Eve — 77: Implanted Brachythearpy Seed Movement due to Transrectal Ultrasound Probe-Induced Prostate Deformation

    SciTech Connect

    Liu, D; Usmani, N; Sloboda, R; Meyer, T; Husain, S; Angyalfi, S; Kay, I

    2014-08-15

    The study investigated the movement of implanted brachytherapy seeds upon transrectal US probe removal, providing insight into the underlying prostate deformation and an estimate of the impact on prostate dosimetry. Implanted seed distributions, one obtained with the prostate under probe compression and another with the probe removed, were reconstructed using C-arm fluoroscopy imaging. The prostate, delineated on ultrasound images, was registered to the fluoroscopy images using seeds and needle tracks identified on ultrasound. A deformation tensor and shearing model was developed to correlate probe-induced seed movement with position. Changes in prostate TG-43 dosimetry were calculated. The model was used to infer the underlying prostate deformation and to estimate the location of the prostate surface in the absence of probe compression. Seed movement patterns upon probe removal reflected elastic decompression, lateral shearing, and rectal bending. Elastic decompression was characterized by expansion in the anterior-posterior direction and contraction in the superior-inferior and lateral directions. Lateral shearing resulted in large anterior movement for extra-prostatic seeds in the lateral peripheral region. Whole prostate D90 increased up to 8 Gy, mainly due to the small but systematic seed movement associated with elastic decompression. For selected patients, lateral shearing movement increased prostate D90 by 4 Gy, due to increased dose coverage in the anterior-lateral region at the expense of the posterior-lateral region. The effect of shearing movement on whole prostate D90 was small compared to elastic decompression due to the subset of peripheral seeds involved, but is expected to have greater consequences for local dose coverage.

  5. Matter under extreme conditions probed by a seeded free-electron-laser

    SciTech Connect

    Bencivenga, F.; Principi, E.; Cucini, R.; Danailov, M. B.; Demidovich, A.; D’Amico, F.; Di Fonzo, S.; Gessini, A.; Kurdi, N.; Mahne, N.; Raimondi, L.; Zangrando, M.; Masciovecchio, C.; Giangrisostomi, E.; Battistoni, A.; Svetina, C.; Di Cicco, A.; Gunnella, R.; Hatada, K.; Filipponi, A.; and others

    2015-08-17

    FERMI is the first user dedicated seeded free-electron-laser (FEL) working in the extreme ultraviolet (XUV) and soft x-ray range. The EIS-TIMEX experimental end-station was availabe to external users since from the beginning of the user operation of the facility, in Dicember 2012. EIS-TIMEX has been conceived to exploit the unique properties of the FERMI source to study matter under extreme and metastable thermodynamic conditions. We hereby report on its basic parameters and applications, which includes very low jitter (i.e., high time resolution) pump-probe measurements.

  6. Matter under extreme conditions probed by a seeded free-electron-laser

    NASA Astrophysics Data System (ADS)

    Bencivenga, F.; Principi, E.; Giangrisostomi, E.; Battistoni, A.; Cucini, R.; Danailov, M. B.; Demidovich, A.; Di Cicco, A.; D'Amico, F.; Di Fonzo, S.; Filipponi, A.; Gessini, A.; Gunnella, R.; Hatada, K.; Kurdi, N.; Mahne, N.; Mincigrucci, R.; Raimondi, L.; Svetina, C.; Zangrando, M.; Masciovecchio, C.

    2015-08-01

    FERMI is the first user dedicated seeded free-electron-laser (FEL) working in the extreme ultraviolet (XUV) and soft x-ray range. The EIS-TIMEX experimental end-station was availabe to external users since from the beginning of the user operation of the facility, in Dicember 2012. EIS-TIMEX has been conceived to exploit the unique properties of the FERMI source to study matter under extreme and metastable thermodynamic conditions. We hereby report on its basic parameters and applications, which includes very low jitter (i.e., high time resolution) pump-probe measurements.

  7. Rapid growth of seed black holes in the early universe by supra-exponential accretion.

    PubMed

    Alexander, Tal; Natarajan, Priyamvada

    2014-09-12

    Mass accretion by black holes (BHs) is typically capped at the Eddington rate, when radiation's push balances gravity's pull. However, even exponential growth at the Eddington-limited e-folding time t(E) ~ few × 0.01 billion years is too slow to grow stellar-mass BH seeds into the supermassive luminous quasars that are observed when the universe is 1 billion years old. We propose a dynamical mechanism that can trigger supra-exponential accretion in the early universe, when a BH seed is bound in a star cluster fed by the ubiquitous dense cold gas flows. The high gas opacity traps the accretion radiation, while the low-mass BH's random motions suppress the formation of a slowly draining accretion disk. Supra-exponential growth can thus explain the puzzling emergence of supermassive BHs that power luminous quasars so soon after the Big Bang. PMID:25103410

  8. Rapid growth of seed black holes in the early universe by supra-exponential accretion

    NASA Astrophysics Data System (ADS)

    Alexander, Tal; Natarajan, Priyamvada

    2014-09-01

    Mass accretion by black holes (BHs) is typically capped at the Eddington rate, when radiation's push balances gravity's pull. However, even exponential growth at the Eddington-limited e-folding time tE ~ few × 0.01 billion years is too slow to grow stellar-mass BH seeds into the supermassive luminous quasars that are observed when the universe is 1 billion years old. We propose a dynamical mechanism that can trigger supra-exponential accretion in the early universe, when a BH seed is bound in a star cluster fed by the ubiquitous dense cold gas flows. The high gas opacity traps the accretion radiation, while the low-mass BH's random motions suppress the formation of a slowly draining accretion disk. Supra-exponential growth can thus explain the puzzling emergence of supermassive BHs that power luminous quasars so soon after the Big Bang.

  9. Tidal disruption as a probe for supermassive black hole binaries

    NASA Astrophysics Data System (ADS)

    Li, Shuo; Liu, Fukun; Berczik, Peter; Spurzem, Rainer

    2016-02-01

    Supermassive black hole binaries (SMBHBs) are the products of frequent galaxy mergers. It is very hard to be detected in quiescent galaxy. By using one million particle direct N-body simulations on special many-core hardware (GPU cluster), we study the dynamical co-evolution of SMBHB and its surrounding stars, specially focusing on the evolution of stellar tidal disruption event (TDE) rates before and after the coalescence of the SMBHB. We find a boosted TDE rate during the merger of the galaxies. After the coalescence of two supermassive black holes (SMBHs), the post-merger SMBH can get a kick velocity due to the anisotropic GW radiations. Our results about the recoiling SMBH, which oscillates around galactic center, show that most of TDEs are contributed by unbound stars when the SMBH passing through galactic center. In addition, the TDE light curve in SMBHB system is significantly different from the curve for single SMBH, which can be used to identify the SMBHB.

  10. Probing topologically charged black holes on brane worlds in f({R}) bulk

    NASA Astrophysics Data System (ADS)

    Kuerten, André M.; da Rocha, Roldão

    2016-07-01

    The perihelion precession, the deflection of light and the radar echo delay are classical tests of General Relativity here used to probe brane-world topologically charged black holes in a f(R) bulk. Moreover, such tests are used to constrain the parameter that arises from the Shiromizu-Maeda-Sasaki procedure applied to a f(R) bulk. Observational data constrain the possible values of the tidal charge parameter and the effective cosmological constant in this context. We show that the observational/experimental data for both perihelion precession and radar echo delay make the black hole parameters to be more strict than the ones for the DMPR black hole. Moreover, the deflection of light constrains the tidal charge parameter similarly as the DMPR black holes, due to a peculiarity in the equation of motion.

  11. Formation of the seed black holes: a role of quark nuggets?

    SciTech Connect

    Lai, X.Y.; Xu, R.X. E-mail: r.x.xu@pku.edu.cn

    2010-05-01

    Strange quark nuggets (SQNs) could be the relics of the cosmological QCD phase transition, and they could very likely be the candidate of cold quark matter if survived the cooling of the later Universe, although the formation and evolution of these SQNs depend on the physical state of the hot QGP (quark-gluon plasma) phase and the state of cold quark matter. We reconsider the possibility of SQNs as cold dark matter, and find that the formation of black holes in primordial halos could be significantly different from the standard scenario. In a primordial halo, the collision between gas and SQNs could be frequent enough, and thus the viscosity acting on each SQN would decrease its angular momentum and make it to sink into the center of the halo, as well as heat the gas. The SQNs with baryon numbers less than 10{sup 35} could assemble in the center of the halo before the formation of primordial stars. A black hole could form by merger of these SQNs, and then its mass could quickly become about 10{sup 3}M{sub s}un or higher, by accreting the surrounding SQNs or gas. The black holes formed in this way could be the seeds for the supermassive black holes at redshift as high as z ∼ 6.

  12. Black Hole Mergers as Probes of Structure Formation

    NASA Technical Reports Server (NTRS)

    Alicea-Munoz, Emily

    2008-01-01

    Observations of gravitational waves from massive black hole (MBH) mergers can provide us with important clues about the era of structure formation in the early universe. Previous research in this field has been limited to calculating merger rates of MBHs using different models where many assumptions are made about the specific values of physical parameters of the mergers, resulting in merger rate estimates that span 5 to 6 orders of magnitude. We develop a semi-analytical, phenomenological model that includes plausible combinations of several physical parameters involved in the mergers. which we then turn around to determine how well LISA observations will be able to enhance our understanding of the universe during the critical z approximately equal to 5-30 structure formation era. We do this by generating synthetic LISA observable data (masses, redshifts, merger rates), which are then analyzed using a Markov Chain Monte Carlo (MCMC) method. This allows us to constrain the physical parameters of the mergers.

  13. SONGLINES FROM DIRECT COLLAPSE SEED BLACK HOLES: EFFECTS OF X-RAYS ON BLACK HOLE GROWTH AND STELLAR POPULATIONS

    SciTech Connect

    Aykutalp, Aycin; Wise, John H.; Spaans, Marco; Meijerink, Rowin

    2014-12-20

    In the last decade, the growth of supermassive black holes (SMBHs) has been intricately linked to galaxy formation and evolution and is a key ingredient in the assembly of galaxies. To investigate the origin of SMBHs, we perform cosmological simulations that target the direct collapse black hole seed formation scenario in the presence of two different strong Lyman-Werner (LW) background fields. These simulations include the X-ray irradiation from a central massive black hole (MBH), H{sub 2} self-shielding, and stellar feedback from metal-free and metal-enriched stars. We find in both simulations that local X-ray feedback induces metal-free star formation ∼0.5 Myr after the MBH forms. The MBH accretion rate reaches a maximum of 10{sup –3} M {sub ☉} yr{sup –1} in both simulations. However, the duty cycle differs and is derived to be 6% and 50% for the high and low LW cases, respectively. The MBH in the high LW case grows only ∼6% in 100 Myr compared to 16% in the low LW case. We find that the maximum accretion rate is determined by the local gas thermodynamics, whereas the duty cycle is determined by the large-scale gas dynamics and gas reservoir. We conclude that radiative feedback from the central MBH plays an important role in star formation in the nuclear regions and stifling initial MBH growth relative to the typical Eddington rate argument, and that initial MBH growth might be affected by the local LW radiation field.

  14. Antiviral effects of black raspberry (Rubus coreanus) seed extract and its polyphenolic compounds on norovirus surrogates.

    PubMed

    Lee, Ji-Hye; Bae, Sun Young; Oh, Mi; Seok, Jong Hyeon; Kim, Sella; Chung, Yeon Bin; Gowda K, Giri; Mun, Ji Young; Chung, Mi Sook; Kim, Kyung Hyun

    2016-06-01

    Black raspberry seeds, a byproduct of wine and juice production, contain large quantities of polyphenolic compounds. The antiviral effects of black raspberry seed extract (RCS) and its fraction with molecular weight less than 1 kDa (RCS-F1) were examined against food-borne viral surrogates, murine norovirus-1 (MNV-1) and feline calicivirus-F9 (FCV-F9). The maximal antiviral effect was achieved when RCS or RCS-F1 was added simultaneously to cells with MNV-1 or FCV-F9, reaching complete inhibition at 0.1-1 mg/mL. Transmission electron microscopy (TEM) images showed enlarged viral capsids or disruption (from 35 nm to up to 100 nm) by RCS-F1. Our results thus suggest that RCS-F1 can interfere with the attachment of viral surface protein to host cells. Further, two polyphenolic compounds derived from RCS-F1, cyanidin-3-glucoside (C3G) and gallic acid, identified by liquid chromatography-tandem mass spectrometry, showed inhibitory effects against the viruses. C3G was suggested to bind to MNV-1 RNA polymerase and to enlarge viral capsids using differential scanning fluorimetry and TEM, respectively. PMID:26983677

  15. Growing massive black holes through supercritical accretion of stellar-mass seeds

    NASA Astrophysics Data System (ADS)

    Lupi, A.; Haardt, F.; Dotti, M.; Fiacconi, D.; Mayer, L.; Madau, P.

    2016-03-01

    The rapid assembly of the massive black holes that power the luminous quasars observed at z ˜ 6-7 remains a puzzle. Various direct collapse models have been proposed to head-start black hole growth from initial seeds with masses ˜105 M⊙, which can then reach a billion solar mass while accreting at the Eddington limit. Here, we propose an alternative scenario based on radiatively inefficient supercritical accretion of stellar-mass holes embedded in the gaseous circumnuclear discs (CNDs) expected to exist in the cores of high-redshift galaxies. Our sub-pc resolution hydrodynamical simulations show that stellar-mass holes orbiting within the central 100 pc of the CND bind to very high density gas clumps that arise from the fragmentation of the surrounding gas. Owing to the large reservoir of dense cold gas available, a stellar-mass black hole allowed to grow at super-Eddington rates according to the `slim-disc' solution can increase its mass by three orders of magnitudes within a few million years. These findings are supported by simulations run with two different hydro codes, RAMSES based on the Adaptive Mesh Refinement technique and GIZMO based on a new Lagrangian Godunov-type method, and with similar, but not identical, sub-grid recipes for star formation, supernova feedback, black hole accretion and feedback. The low radiative efficiency of supercritical accretion flows are instrumental to the rapid mass growth of our black holes, as they imply modest radiative heating of the surrounding nuclear environment.

  16. [Physical and antioxidant characteristics of black (Brassica nigra) and yellow mustard (Brassica alba) seeds and their products].

    PubMed

    Mejia-Garibay, Beatriz; Guerrero-Beltrán, José Ángel; Palou, Enrique; López-Malo, Aurelio

    2015-06-01

    The composition, some physical properties (density, refraction index, and color), antioxidant capacity (DPPH), and fatty acid profile of seeds of black (Brassica nigra) or yellow mustard (Brassica alba) were evaluated, as well as for their oils and residues from oil extraction. Density of the black and yellow mustard oils were 0.912 ± 0.01 and 0.916 ± 0.01 g/mL, respectively; their refraction indexes were 1.4611 ± 0.01 and 1.4617 ± 0.01, respectively; being not significantly different (p > 0.05) between two mustards. Color parameters of the black and yellow mustard oils presented greenish-yellow tones and reddish-yellow tones, respectively; regarding antioxidant activities, these ranged from 25 mg equivalents of Trolox/100 gin the yellow mustard oil to 1,366 mg equivalents of Trolox/100 g in the residues from oil extraction of black seed mustard. The fatty acid profile of the black mustard seed revealed that its predomipant fatty acid is oleic (22.96%), followed by linoleic (6.63%) and linolenic (3.22%), whereas foryellow mustard seed the major fatty acid is erucic (6.87%), followed by oleic (5.08%) and linoleic (1.87%) acids. PMID:26817385

  17. Black Hole Mergers as Probes of Structure Formation

    NASA Technical Reports Server (NTRS)

    Alicea-Munoz, E.; Miller, M. Coleman

    2008-01-01

    Intense structure formation and reionization occur at high redshift, yet there is currently little observational information about this very important epoch. Observations of gravitational waves from massive black hole (MBH) mergers can provide us with important clues about the formation of structures in the early universe. Past efforts have been limited to calculating merger rates using different models in which many assumptions are made about the specific values of physical parameters of the mergers, resulting in merger rate estimates that span a very wide range (0.1 - 104 mergers/year). Here we develop a semi-analytical, phenomenological model of MBH mergers that includes plausible combinations of several physical parameters, which we then turn around to determine how well observations with the Laser Interferometer Space Antenna (LISA) will be able to enhance our understanding of the universe during the critical z 5 - 30 structure formation era. We do this by generating synthetic LISA observable data (total BH mass, BH mass ratio, redshift, merger rates), which are then analyzed using a Markov Chain Monte Carlo method. This allows us to constrain the physical parameters of the mergers. We find that our methodology works well at estimating merger parameters, consistently giving results within 1- of the input parameter values. We also discover that the number of merger events is a key discriminant among models. This helps our method be robust against observational uncertainties. Our approach, which at this stage constitutes a proof of principle, can be readily extended to physical models and to more general problems in cosmology and gravitational wave astrophysics.

  18. Horizon growth of supermassive black hole seeds fed with collisional dark matter

    NASA Astrophysics Data System (ADS)

    Lora-Clavijo, F. D.; Gracia-Linares, M.; Guzmán, F. S.

    2014-09-01

    We present the accretion of collisional dark matter on a supermassive black hole (SMBH) seed. The analysis is based on the numerical solution of the fully coupled system of Einstein-Euler equations for spherically symmetric flow, where the dark matter is modelled as a perfect fluid that obeys an ideal gas equation of state. As the black hole actually grows, the accretion rate of dark matter corresponds to the black hole apparent horizon growth rate. We analyse cases with infall velocity as high as 0.5c and an environment density of 100 M⊙ pc-3, which are rather extreme conditions. Being the radial flux the maximum accretion case, our results show that the accretion of an ideal gas, eventually collisional dark matter, does not contribute significantly to SMBH masses. This result favours models predicting SMBHs were formed already with supermasses. We show that despite the fact that we are solving the full general relativistic system, for the parameter space studied our results are surprisingly similar to those obtained using the Bondi formula, which somehow certifies its use as a good approximation of a fully evolving space-time with spherical symmetry at short scales at least for dark matter densities. Additionally, we study the density profile of the gas and find that the presence of SMBHs redistributes the gas near the event horizon with a cuspy profile, whereas beyond a small fraction of a parsec it is not cuspy anymore.

  19. Supermassive black hole seed formation at high redshifts: long-term evolution of the direct collapse

    NASA Astrophysics Data System (ADS)

    Shlosman, Isaac; Choi, Jun-Hwan; Begelman, Mitchell C.; Nagamine, Kentaro

    2016-02-01

    We use cosmological adaptive mesh refinement code ENZO zoom-in simulations to study the long-term evolution of the collapsing gas within dark matter haloes at z. This direct collapse process is a leading candidate for rapid formation of supermassive black hole (SMBH) seeds. To circumvent the Courant condition at small radii, we apply the sink particle method, focusing on evolution on scales ˜0.01-10 pc. The collapse proceeds in two stages, with the secondary runaway happening within the central 10 pc. The sink particles form when the collapsing gas requires additional refinement of the grid size at the highest refinement level. Their growth is negligible with the sole exception of the central seed which grows dramatically to Mseed ˜ 2 × 106 M⊙ in ˜2 Myr, confirming the feasibility of this path to the SMBH. The variability of angular momentum in the accreted gas results in the formation of two misaligned discs. Both discs lie within the Roche limit of the central seed. While the inner disc is geometrically thin and weakly asymmetric, the outer disc flares due to turbulent motions as a result of the massive inflow along a pair of penetrating filaments. The filamentary inflow determines the dominant Fourier modes in this disc - these modes have a non-self-gravitational origin. We do not confirm that m = 1 is a dominant mode that drives the inflow in the presence of a central massive object. The overall configuration appears to be generic, and is expected to form when the central seed becomes sufficiently massive.

  20. Immunocytochemical Localization of Mandelonitrile Lyase in Mature Black Cherry (Prunus serotina Ehrh.) Seeds 1

    PubMed Central

    Wu, Hua-Cheng; Poulton, Jonathan E.

    1991-01-01

    Mandelonitrile lyase (MDL, EC 4.1.2.10), which catalyzes the reversible dissociation of (R)-(+)-mandelonitrile to benzaldehyde and hydrogen cyanide, was purified to apparent homogeneity from mature black cherry (Prunus serotina Ehrh.) seeds by conventional protein purification techniques. This flavoprotein is monomeric with a subunit molecular mass of 57 kilodaltons. Glycoprotein character was shown by its binding to the affinity matrix concanavalin A-Sepharose 4B with subsequent elution by α-methyl-d-glucoside. Upon chemical deglycosylation by trifluoromethanesulfonic acid, the molecular mass was reduced to 50.9 kilodaltons. Two-dimensional gel analysis of deglycosylated MDL revealed the presence of several subunit isoforms of similar molecular mass but differing slightly in isoelectric point. Polyclonal antibodies were raised in New Zealand white rabbits against deglycosylated and untreated MDL. Antibody titers were determined by enzyme linked immunosorbent and dot immunobinding assays, while their specificities were assessed by Western immunoblot analysis. Antibodies raised against untreated lyase recognized several proteins in addition to MDL. In contrast, antisera raised against deglycosylated MDL were monospecific and were utilized for developmental and immunocytochemical localization studies. SDS-PAGE and immunoblotting analysis of seed proteins during fruit maturation showed that MDL first appeared in seeds shortly after cotyledons began development. In cotyledon cells of mature seeds, MDL was localized primarily in the cell wall with lesser amounts in the protein bodies, whereas in endosperm cells, this labeling pattern was reversed. N-terminal sequence data was gathered for future molecular approaches to the question of MDL microheterogeneity. ImagesFigure 1Figure 2Figure 3Figure 4Figure 5Figure 6 PMID:16668338

  1. ToF-SIMS applied to probe bixin in Bixa orellana seeds

    NASA Astrophysics Data System (ADS)

    Houssiau, L.; Felicissimo, M.; Bittencourt, C.; Pireaux, J. J.

    2004-06-01

    Due to the high daily intake of Annatto, a natural food colorant, it is important to determine its constituents in order to attain some information of nutritional significance. First, an Annatto extract was obtained. The ToF-SIMS spectra measured after depositing a drop of this extract on a silicon substrate exhibit strong molecular peaks clearly related to the bixin molecule (C 25H 30O 4), which has a molar mass M=394. The most intense characteristic ion appears at m/ z=396 ( M+2), but other molecular ions (e.g. at m/ z=337 obtained by the loss of C 2O 2H or even a dimer at m/ z=790, or 2 M+2) were assigned to the bixin. On a silver substrate, M+Ag cations are also observed, confirming the straightforward identification of the colorant. The spectra recorded on the crude seed were remarkably similar to those obtained on the extract confirming the technique's ability to probe the seed's chemistry without preliminary treatment. After a prolonged storage in ambient light, a drastic reduction of the bixin molecular ion is observed, along with the appearance of xylene fragments.

  2. Probing the Accretion Geometry of Black Holes with X-Ray Polarization

    NASA Technical Reports Server (NTRS)

    Schnitman, Jeremy D.

    2011-01-01

    In the coming years, new space missions will be able to measure X-ray polarization at levels of 1% or better in the approx.1-10 keV energy band. In particular, X-ray polarization is an ideal tool for determining the nature of black hole (BH) accretion disks surrounded by hot coronae. Using a Monte Carlo radiation transport code in full general relativity, we calculate the spectra and polarization features of these BH systems. At low energies, the signal is dominated by the thermal flux coming directly from the optically thick disk. At higher energies, the thermal seed photons have been inverse-Compton scattered by the corona, often reflecting back off the disk before reaching the observer, giving a distinctive polarization signature. By measuring the degree and angle of this X-ray polarization, we can infer the BH inclination, the emission geometry of the accretion flow, and also determine the spin of the black hole.

  3. EVIDENCE FOR THREE ACCRETING BLACK HOLES IN A GALAXY AT z {approx} 1.35: A SNAPSHOT OF RECENTLY FORMED BLACK HOLE SEEDS?

    SciTech Connect

    Schawinski, Kevin; Urry, Meg; Treister, Ezequiel; Simmons, Brooke; Natarajan, Priyamvada; Glikman, Eilat

    2011-12-20

    One of the key open questions in cosmology today pertains to understanding when, where, and how supermassive black holes form. While it is clear that mergers likely play a significant role in the growth cycles of black holes, the issue of how supermassive black holes form, and how galaxies grow around them, still needs to be addressed. Here, we present Hubble Space Telescope Wide Field Camera 3/IR grism observations of a clumpy galaxy at z = 1.35, with evidence for 10{sup 6}-10{sup 7} M{sub Sun} rapidly growing black holes in separate sub-components of the host galaxy. These black holes could have been brought into close proximity as a consequence of a rare multiple galaxy merger or they could have formed in situ. Such holes would eventually merge into a central black hole as the stellar clumps/components presumably coalesce to form a galaxy bulge. If we are witnessing the in situ formation of multiple black holes, their properties can inform seed formation models and raise the possibility that massive black holes can continue to emerge in star-forming galaxies as late as z = 1.35 (4.8 Gyr after the big bang).

  4. Black howler monkey (Alouatta pigra) activity, foraging and seed dispersal patterns in shaded cocoa plantations versus rainforest in southern Mexico.

    PubMed

    Zárate, Diego A; Andresen, Ellen; Estrada, Alejandro; Serio-Silva, Juan Carlos

    2014-09-01

    Recent evidence has shown that primates worldwide use agroecosystems as temporary or permanent habitats. Detailed information on how these primates are using these systems is scant, and yet their role as seed dispersers is often implied. The main objective of this study was to compare the activity, foraging patterns and seed dispersal role of black howler monkeys (Alouatta pigra) inhabiting shaded cocoa plantations and rainforest in southern Chiapas, Mexico. We gathered data on three monkey groups living in shaded cocoa plantations and three groups living in rainforest, using focal sampling, and collecting fecal samples. General activity and foraging patterns were similar in both habitats, with the exception that monkeys in the cocoa habitat spent more time feeding on petioles. Monkeys in shaded cocoa plantations dispersed 51,369 seeds (4% were seeds ≥3 mm width) of 16 plant species. Monkeys in the rainforest dispersed 6,536 seeds (78% were seeds ≥3 mm width) of 13 plant species. Our data suggest that the difference between habitats in the proportion of large versus small seeds dispersed reflects differences in fruit species abundance and availability in cocoa versus forest. Mean seed dispersal distances were statistically similar in both habitats (cocoa = 149 m, forest = 86 m). We conclude that the studied cocoa plantations provide all elements necessary to constitute a long-term permanent habitat for black howler monkeys. In turn, howler monkeys living in these plantations are able to maintain their functional role as seed dispersers for those native tree and liana species present within their areas of activities. PMID:24668557

  5. Black cumin seed essential oil, as a potent analgesic and antiinflammatory drug.

    PubMed

    Hajhashemi, Valiollah; Ghannadi, Alireza; Jafarabadi, Hadi

    2004-03-01

    The steam-distilled essential oil of Iranian black cumin seed (Nigella sativa L.) was investigated for its composition and analgesic and antiinflammatory properties. After oil analysis by GC/MS, 20 compounds were identified in the oil, obtained in 0.4% (v/w) yield. Among them, para-cymene (37.3%) and thymoquinone (13.7%) were the major components. Acetic acid-induced writhing, formalin and light tail flick tests were used for assessment of analgesic activity. Antiinflammatory activity was evaluated using carrageenan-induced paw oedema in rats and croton oil-induced ear oedema in mice. Black cumin seed essential oil (BCSEO) was found to produce a significant analgesic effect in acetic acid-induced writhing, formalin and light tail flick tests. Naloxone, an opioid antagonist, could not reverse the analgesic effect observed in the formalin test. Although oral administration of BCSEO at doses of 100, 200 and 400 micro L/kg did not exert a significant antiinflammatory effect in the carrageenan test, i.p. injection of the same doses significantly (p < 0.001) inhibited carrageenan-induced paw oedema. BCSEO at doses of 10 and 20 micro L/ear could also reduce croton oil-induced oedema. It seems that mechanism(s) other than opioid receptors is (are) involved in the analgesic effect of BCSEO since naloxone could not reverse this effect. Both systemic and local administration of BCSEO showed antiinflammatory activity. Thymoquinone, as one of the major components of BCSEO, probably has an important role in these pharmacological effects. PMID:15103664

  6. Water entry for the black locust (Robinia pseudoacacia L.) seeds observed by dedicated micro-magnetic resonance imaging.

    PubMed

    Koizumi, Mika; Kano, Hiromi

    2016-07-01

    Water entry at germination for black locust (Robinia pseudoacacia L.) seeds which are known as hard seeds with impermeable seed coat to water, was examined using micro-magnetic resonance imaging (MRI). The MRI apparatus equipped with a low-field (1 T; Tesla) permanent magnet was used, which is open access, easy maintenance, operable and transportable. The excellent point of the apparatus is that T 1-enhancement of water signals absorbed in dry seeds against steeping free water is stronger than the apparatuses with high-field superconducting magnets, which enabled clear detection of water entry. Water hardly penetrated into the seeds for more than 8 h but approximately 60 % of seeds germinated by incubating on wet filter papers for several days. Hot water treatments above 75 °C for 3 min effectively induced water gap; scarification was 70 % at 100 °C and 75 °C, declined to 15 % at 50 °C and decreased further at room temperature. Water entered into the scarified seeds exclusively through the lens, spread along the dorsal side of the seeds and reached the hypocotyl, whereas water migrated slowly through hilum side to radicle within 3 h. PMID:27059756

  7. Locations of radical species in black pepper seeds investigated by CW EPR and 9 GHz EPR imaging

    NASA Astrophysics Data System (ADS)

    Nakagawa, Kouichi; Epel, Boris

    2014-10-01

    In this study, noninvasive 9 GHz electron paramagnetic resonance (EPR)-imaging and continuous wave (CW) EPR were used to investigate the locations of paramagnetic species in black pepper seeds without further irradiation. First, lithium phthalocyanine (LiPC) phantom was used to examine 9 GHz EPR imaging capabilities. The 9 GHz EPR-imager easily resolved the LiPC samples at a distance of ∼2 mm. Then, commercially available black pepper seeds were measured. We observed signatures from three different radical species, which were assigned to stable organic radicals, Fe3+, and Mn2+ complexes. In addition, no EPR spectral change in the seed was observed after it was submerged in distilled H2O for 1 h. The EPR and spectral-spatial EPR imaging results suggested that the three paramagnetic species were mostly located at the seed surface. Fewer radicals were found inside the seed. We demonstrated that the CW EPR and 9 GHz EPR imaging were useful for the determination of the spatial distribution of paramagnetic species in various seeds.

  8. Phenology, fruit production and seed dispersal of Astrocaryum jauari (Arecaceae) in Amazonian black water floodplains.

    PubMed

    Piedade, Maria Teresa F; Parolin, Pia; Junk, Wolfgang J

    2006-12-01

    Astrocaryum jauari Mart. (Arecaceae) is one of the commonest palm species occurring in nutritionally poor Amazonian black water floodplains. It is an emergent or subcanopy tree that grows on river banks and islands, with a wide distribution along the entire flooding gradient, tolerating flood durations between 30 and 340 days. The species is important for fish nutrition in the floodplains, and is also used for hearts of palm. In the present study, the auto-ecology of A. jauari was analysed over a period of two years in the Anavilhanas Archipelago, Rio Negro, Brazil, with a focus on phenology, fruit production, and seed dispersal. Fruit fall is annual and synchronized with high water levels, with a production of 1.6 ton of fruit ha(-1). The fruits are eaten by at least 16 species of fish which either gnaw the pulp, fragment the seed, or ingest the entire fruit, thus acting as dispersal agents. Besides ichthyocory, barochory (with subsequent vegetative propagation) is an important dispersal mode, enhancing the occurrence of large masses of individuals in the Anavilhanas islands and in the region of maximum palm heart extraction near Barcelos. PMID:18457155

  9. THE FORMATION OF SUPERMASSIVE BLACK HOLES FROM LOW-MASS POP III SEEDS

    SciTech Connect

    Whalen, Daniel J.; Fryer, Chris L.

    2012-09-01

    The existence of 10{sup 9} M{sub Sun} black holes (BHs) in massive galaxies by z {approx} 7 is one of the great unsolved mysteries in cosmological structure formation. One theory argues that they originate from the BHs of Pop III stars at z {approx} 20 and then accrete at the Eddington limit down to the epoch of reionization, which requires that they have constant access to rich supplies of fuel. Because early numerical simulations suggested that Pop III stars were {approx}>100 M{sub Sun }, the supermassive black hole (SMBH) seeds considered up to now were 100-300 M{sub Sun }. However, there is a growing numerical and observational consensus that some Pop III stars were tens of solar masses, not hundreds, and that 20-40 M{sub Sun} BHs may have been much more plentiful at high redshift. However, we find that natal kicks imparted to 20-40 M{sub Sun} Pop III BHs during formation eject them from their halos and hence their fuel supply, precluding them from Eddington-limit growth. Consequently, SMBHs are far less likely to form from low-mass Pop III stars than from very massive ones.

  10. Gravitational-wave background as a probe of the primordial black-hole abundance.

    PubMed

    Saito, Ryo; Yokoyama, Jun'ichi

    2009-04-24

    The formation of a significant number of black holes (PBHs) is realized if and only if primordial density fluctuations have a large amplitude, which means that tensor perturbations generated from these scalar perturbations as a second-order effect are also large and comparable to the observational data. We show that pulsar timing data essentially rule out PBHs with 10;{2}-10;{4}M_{middle dot in circle}, which were previously considered as a candidate of intermediate-mass black holes, and that PBHs with a mass range of 10;{20} to 10;{26} g, which serves as a candidate of dark matter, may be probed by future space-based laser interferometers and atomic interferometers. PMID:19518692

  11. Hawkmoth pollinators decrease seed set of a low-nectar Petunia axillaris line through reduced probing time.

    PubMed

    Brandenburg, Anna; Kuhlemeier, Cris; Bshary, Redouan

    2012-09-11

    Although deception of floral pollinators is well known among orchids, the majority of animal-pollinated plants secure pollination by nectar rewards. The costs and benefits of nectar production remain poorly understood. Here, we developed a crossing design to introgress a low-nectar-volume locus of Petunia integrifolia into the genetic background of P. axillaris. The resulting introgression line resembled P. axillaris but produced only one-third of the nectar volume. When exposed simultaneously to low-nectar and wild-type P. axillaris plants, hawkmoth pollinators reduced their probing duration on low-nectar plants but otherwise did not show any signs of discrimination against these plants. However, reduced probing duration resulted in reduced seed production in the low-nectar plants despite their higher reproductive potential as evidenced by hand pollination. In line with this interpretation, we found a positive correlation between probing duration and seed set, and hawkmoth pollination of low-nectar plants that were manually supplemented with nectar to parental levels yielded seed sets similar to hand pollination. Thus, a simple self-serving pollinator behavior--the adjustment of probing time in response to nectar volume--may select against reducing nectar and protect many plant-pollinator mutualisms against a drift toward parasitism. PMID:22840518

  12. Understanding X-ray Reflection as a Probe of Accreting Black Holes

    NASA Astrophysics Data System (ADS)

    Wilkins, Dan

    2014-01-01

    Active galactic nuclei (AGN) are some of the most luminous objects we see in the Universe, powered by the accretion of matter onto a supermassive black hole in the centre of a galaxy, yet many of the physical processes by which the energy is released and injected into the surroundings remain a mystery. X-rays are emitted from a ‘corona’ of energetic particles surrounding the black hole and as well as being observed directly, they are seen to be reflected from the accreting disc, producing a number of spectral features including emission lines that are broadened by relativistic effects in the proximity of the black hole. In my thesis, I develop methods through which detailed measurement of the reflected X-rays from the accretion disc can be used to probe the innermost regions of accretion flow and corona, right down to the innermost stable orbit and the event horizon. Novel spectral analysis techniques allow us to reconstruct, from the observed relativistic X-ray reflection spectrum the spatially resolved illumination pattern of the accretion disc and will discuss how comparing this to the results of systematic general relativistic ray tracing simulations I have developed, we are able to constrain the location and geometry of the X-ray emitting corona and understand the dramatic change of the narrow line Seyfert 1 galaxy 1H 0707-495 into an extremely low flux state in terms of a collapse in the corona. I will discuss how measurements of the X-ray variability, specifically the reverberation time lags that are observed between variability in the directly observed X-rays from the corona and those reflected from the accretion disc add a further dimension to the study of accreting black holes, letting us not only build up a three dimensional image of the immediate vicinity of the black hole but also to probe mechanisms by which the energy is released from the accretion flow; techniques that will let us exploit not just current instrumentation but future proposed X

  13. Comparative effects of using black seed (Nigella sativa), cumin seed (Cuminum cyminum), probiotic or prebiotic on growth performance, blood haematology and serum biochemistry of broiler chicks.

    PubMed

    Alimohamadi, K; Taherpour, K; Ghasemi, H A; Fatahnia, F

    2014-06-01

    A 42-day trial was conducted to compare the effects of the following seven experimental diets, which varied in black seed, cumin seed, probiotic or prebiotic concentrations, on the broiler chicks: control (no additives), diet BS1 (4 g/kg black seed), diet BS2 (8 g/kg black seed), diet CS1 (4 g/kg cumin seed), diet CS2 (8 g/kg cumin seed), diet Pro (1 g/kg probiotic Primalac(®)) and diet Pre (2 g/kg prebiotic Fermacto(®)). A total of 420 1-day-old male broiler chicks, initially weighing an average of 43 g, were distributed into 28 floor pens at a stocking density of 15 birds per pen. At 28 day of age, the body weight in the birds fed diets BS2, CS2 and Pro was significantly higher than in the control group, but final body weight was not affected. Additionally, the birds fed diets BS2, Pro and Pre exhibited better feed conversion ratio than control birds from 0 to 42 day of age. Diets BS2, CS2 and Pro also statistically increased the relative weight of thymus and bursa of Fabricius, whereas only diet Pro decreased the abdominal fat percentage compared with control diet. Regarding the haematological parameters, feeding diet BS2 yielded a significant increase in red blood cell count, haemoglobin concentration and haematocrit percentage compared with control diet. Serum total cholesterol and low-density lipoprotein cholesterol levels in the birds fed diets BS2, Pro and Pre were also significantly lower than in the birds fed the control diet. Without exception, no diets affected feed intake, internal organs weights, carcass characteristics, antibody titres against Newcastle and influenza viruses and leucocyte subsets. In general, current study showed promising results regarding the use of spice additives as growth and health promoters, especially at higher levels of their incorporation in the diets, which were comparable to the probiotic- or prebiotic-containing diets. PMID:23909469

  14. Phytochemicals of black bean seed coats: isolation, structure elucidation, and their antiproliferative and antioxidative activities.

    PubMed

    Dong, Mei; He, Xiangjiu; Liu, Rui Hai

    2007-07-25

    Bioactivity-guided fractionation of black bean (Phaseolus vulgaris) seed coats was used to determine the chemical identity of bioactive constituents, which showed potent antiproliferative and antioxidative activities. Twenty-four compounds including 12 triterpenoids, 7 flavonoids, and 5 other phytochemicals were isolated using gradient solvent fractionation, silica gel and ODS columns, and semipreparative and preparative HPLC. Their chemical structures were identified using MS, NMR, and X-ray diffraction analysis. Antiproliferative activities of isolated compounds against Caco-2 human colon cancer cells, HepG2 human liver cancer cells, and MCF-7 human breast cancer cells were evaluated. Among the compounds isolated, compounds 1, 2, 6, 7, 8, 13, 14, 15, 16, 19, and 20 showed potent inhibitory activities against the proliferation of HepG2 cells, with EC50 values of 238.8 +/- 19.2, 120.6 +/- 7.3, 94.4 +/- 3.4, 98.9 +/- 3.3, 32.1 +/- 6.3, 306.4 +/- 131.3, 156.9 +/- 11.8, 410.3 +/- 17.4, 435.9 +/- 47.7, 202.3 +/- 42.9, and 779.3 +/- 37.4 microM, respectively. Compounds 1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 14, 15, 19, and 20 showed potent antiproliferative activities against Caco-2 cell growth, with EC50 values of 179.9 +/- 16.9, 128.8 +/- 11.6, 197.8 +/- 4.2, 105.9 +/- 4.7, 13.9 +/- 2.8, 35.1 +/- 2.9, 31.2 +/- 0.5, 71.1 +/- 11.9, 40.8 +/- 4.1, 55.7 +/- 8.1, 299.8 +/- 17.3, 533.3 +/- 126.0, 291.2 +/- 1.0, and 717.2 +/- 104.8 microM, respectively. Compounds 5, 7, 8, 9, 11, 19, 20 showed potent antiproliferative activities against MCF-7 cell growth in a dose-dependent manner, with EC50 values of 129.4 +/- 9.0, 79.5 +/- 1.0, 140.1 +/- 31.8, 119.0 +/- 7.2, 84.6 +/- 1.7, 186.6 +/- 21.1, and 1308 +/- 69.9 microM, respectively. Six flavonoids (compounds 14-19) showed potent antioxidant activity. These results showed the phytochemical extracts of black bean seed coats have potent antioxidant and antiproliferative activities. PMID:17602653

  15. Widely tunable two-colour seeded free-electron laser source for resonant-pump resonant-probe magnetic scattering.

    PubMed

    Ferrari, Eugenio; Spezzani, Carlo; Fortuna, Franck; Delaunay, Renaud; Vidal, Franck; Nikolov, Ivaylo; Cinquegrana, Paolo; Diviacco, Bruno; Gauthier, David; Penco, Giuseppe; Ribič, Primož Rebernik; Roussel, Eleonore; Trovò, Marco; Moussy, Jean-Baptiste; Pincelli, Tommaso; Lounis, Lounès; Manfredda, Michele; Pedersoli, Emanuele; Capotondi, Flavio; Svetina, Cristian; Mahne, Nicola; Zangrando, Marco; Raimondi, Lorenzo; Demidovich, Alexander; Giannessi, Luca; De Ninno, Giovanni; Danailov, Miltcho Boyanov; Allaria, Enrico; Sacchi, Maurizio

    2016-01-01

    The advent of free-electron laser (FEL) sources delivering two synchronized pulses of different wavelengths (or colours) has made available a whole range of novel pump-probe experiments. This communication describes a major step forward using a new configuration of the FERMI FEL-seeded source to deliver two pulses with different wavelengths, each tunable independently over a broad spectral range with adjustable time delay. The FEL scheme makes use of two seed laser beams of different wavelengths and of a split radiator section to generate two extreme ultraviolet pulses from distinct portions of the same electron bunch. The tunability range of this new two-colour source meets the requirements of double-resonant FEL pump/FEL probe time-resolved studies. We demonstrate its performance in a proof-of-principle magnetic scattering experiment in Fe-Ni compounds, by tuning the FEL wavelengths to the Fe and Ni 3p resonances. PMID:26757813

  16. Widely tunable two-colour seeded free-electron laser source for resonant-pump resonant-probe magnetic scattering

    NASA Astrophysics Data System (ADS)

    Ferrari, Eugenio; Spezzani, Carlo; Fortuna, Franck; Delaunay, Renaud; Vidal, Franck; Nikolov, Ivaylo; Cinquegrana, Paolo; Diviacco, Bruno; Gauthier, David; Penco, Giuseppe; Ribič, Primož Rebernik; Roussel, Eleonore; Trovò, Marco; Moussy, Jean-Baptiste; Pincelli, Tommaso; Lounis, Lounès; Manfredda, Michele; Pedersoli, Emanuele; Capotondi, Flavio; Svetina, Cristian; Mahne, Nicola; Zangrando, Marco; Raimondi, Lorenzo; Demidovich, Alexander; Giannessi, Luca; de Ninno, Giovanni; Danailov, Miltcho Boyanov; Allaria, Enrico; Sacchi, Maurizio

    2016-01-01

    The advent of free-electron laser (FEL) sources delivering two synchronized pulses of different wavelengths (or colours) has made available a whole range of novel pump-probe experiments. This communication describes a major step forward using a new configuration of the FERMI FEL-seeded source to deliver two pulses with different wavelengths, each tunable independently over a broad spectral range with adjustable time delay. The FEL scheme makes use of two seed laser beams of different wavelengths and of a split radiator section to generate two extreme ultraviolet pulses from distinct portions of the same electron bunch. The tunability range of this new two-colour source meets the requirements of double-resonant FEL pump/FEL probe time-resolved studies. We demonstrate its performance in a proof-of-principle magnetic scattering experiment in Fe-Ni compounds, by tuning the FEL wavelengths to the Fe and Ni 3p resonances.

  17. Widely tunable two-colour seeded free-electron laser source for resonant-pump resonant-probe magnetic scattering

    PubMed Central

    Ferrari, Eugenio; Spezzani, Carlo; Fortuna, Franck; Delaunay, Renaud; Vidal, Franck; Nikolov, Ivaylo; Cinquegrana, Paolo; Diviacco, Bruno; Gauthier, David; Penco, Giuseppe; Ribič, Primož Rebernik; Roussel, Eleonore; Trovò, Marco; Moussy, Jean-Baptiste; Pincelli, Tommaso; Lounis, Lounès; Manfredda, Michele; Pedersoli, Emanuele; Capotondi, Flavio; Svetina, Cristian; Mahne, Nicola; Zangrando, Marco; Raimondi, Lorenzo; Demidovich, Alexander; Giannessi, Luca; De Ninno, Giovanni; Danailov, Miltcho Boyanov; Allaria, Enrico; Sacchi, Maurizio

    2016-01-01

    The advent of free-electron laser (FEL) sources delivering two synchronized pulses of different wavelengths (or colours) has made available a whole range of novel pump–probe experiments. This communication describes a major step forward using a new configuration of the FERMI FEL-seeded source to deliver two pulses with different wavelengths, each tunable independently over a broad spectral range with adjustable time delay. The FEL scheme makes use of two seed laser beams of different wavelengths and of a split radiator section to generate two extreme ultraviolet pulses from distinct portions of the same electron bunch. The tunability range of this new two-colour source meets the requirements of double-resonant FEL pump/FEL probe time-resolved studies. We demonstrate its performance in a proof-of-principle magnetic scattering experiment in Fe–Ni compounds, by tuning the FEL wavelengths to the Fe and Ni 3p resonances. PMID:26757813

  18. Anticancer activity of Nigella sativa (black seed) and its relationship with the thermal processing and quinone composition of the seed.

    PubMed

    Agbaria, Riad; Gabarin, Adi; Dahan, Arik; Ben-Shabat, Shimon

    2015-01-01

    The traditional preparation process of Nigella sativa (NS) oil starts with roasting of the seeds, an allegedly unnecessary step that was never skipped. The aims of this study were to investigate the role and boundaries of thermal processing of NS seeds in the preparation of therapeutic extracts and to elucidate the underlying mechanism. NS extracts obtained by various seed thermal processing methods were investigated in vitro for their antiproliferative activity in mouse colon carcinoma (MC38) cells and for their thymoquinone content. The effect of the different methods of thermal processing on the ability of the obtained NS oil to inhibit the nuclear factor kappa B (NF-κB) pathway was then investigated in Hodgkin's lymphoma (L428) cells. The different thermal processing protocols yielded three distinct patterns: heating the NS seeds to 50°C, 100°C, or 150°C produced oil with a strong ability to inhibit tumor cell growth; no heating or heating to 25°C had a mild antiproliferative effect; and heating to 200°C or 250°C had no effect. Similar patterns were obtained for the thymoquinone content of the corresponding oils, which showed an excellent correlation with the antiproliferative data. It is proposed that there is an oxidative transition mechanism between quinones after controlled thermal processing of the seeds. While NS oil from heated seeds delayed the expression of NF-κB transcription, non-heated seeds resulted in only 50% inhibition. The data indicate that controlled thermal processing of NS seeds (at 50°C-150°C) produces significantly higher anticancer activity associated with a higher thymoquinone oil content, and inhibits the NF-κB signaling pathway. PMID:26124636

  19. Anticancer activity of Nigella sativa (black seed) and its relationship with the thermal processing and quinone composition of the seed

    PubMed Central

    Agbaria, Riad; Gabarin, Adi; Dahan, Arik; Ben-Shabat, Shimon

    2015-01-01

    The traditional preparation process of Nigella sativa (NS) oil starts with roasting of the seeds, an allegedly unnecessary step that was never skipped. The aims of this study were to investigate the role and boundaries of thermal processing of NS seeds in the preparation of therapeutic extracts and to elucidate the underlying mechanism. NS extracts obtained by various seed thermal processing methods were investigated in vitro for their antiproliferative activity in mouse colon carcinoma (MC38) cells and for their thymoquinone content. The effect of the different methods of thermal processing on the ability of the obtained NS oil to inhibit the nuclear factor kappa B (NF-κB) pathway was then investigated in Hodgkin’s lymphoma (L428) cells. The different thermal processing protocols yielded three distinct patterns: heating the NS seeds to 50°C, 100°C, or 150°C produced oil with a strong ability to inhibit tumor cell growth; no heating or heating to 25°C had a mild antiproliferative effect; and heating to 200°C or 250°C had no effect. Similar patterns were obtained for the thymoquinone content of the corresponding oils, which showed an excellent correlation with the antiproliferative data. It is proposed that there is an oxidative transition mechanism between quinones after controlled thermal processing of the seeds. While NS oil from heated seeds delayed the expression of NF-κB transcription, non-heated seeds resulted in only 50% inhibition. The data indicate that controlled thermal processing of NS seeds (at 50°C–150°C) produces significantly higher anticancer activity associated with a higher thymoquinone oil content, and inhibits the NF-κB signaling pathway. PMID:26124636

  20. Differential accumulation of phenolic compounds and expression of related genes in black- and yellow-seeded Brassica napus

    PubMed Central

    Qu, Cunmin; Fu, Fuyou; Lu, Kun; Zhang, Kai; Wang, Rui; Xu, Xinfu; Wang, Min; Lu, Junxing; Wan, Huafang; Zhanglin, Tang; Li, Jiana

    2013-01-01

    Developing yellow-seeded Brassica napus (rapeseed) with improved qualities is a major breeding goal. The intermediate and final metabolites of the phenylpropanoid and flavonoid pathways affect not only oil quality but also seed coat colour of B. napus. Here, the accumulation of phenolic compounds was analysed in the seed coats of black-seeded (ZY821) and yellow-seeded (GH06) B. napus. Using toluidine blue O staining and liquid chromatography–mass spectrometry, histochemical and biochemical differences were identified in the accumulation of phenolic compounds between ZY821 and GH06. Two and 13 unique flavonol derivatives were detected in ZY821 and GH06, respectively. Quantitative real-time PCR analysis revealed significant differences between ZY821 and GH06 in the expression of common phenylpropanoid biosynthetic genes (BnPAL and BnC4H), common flavonoid biosynthetic genes (BnTT4 and BnTT6), anthocyanin- and proanthocyandin-specific genes (BnTT3 and BnTT18), proanthocyandin-specific genes (BnTT12, BnTT10, and BnUGT2) and three transcription factor genes (BnTTG1, BnTTG2, and BnTT8) that function in the flavonoid biosynthetic pathway. These data provide insight into pigment accumulation in B. napus, and serve as a useful resource for researchers analysing the formation of seed coat colour and the underlying regulatory mechanisms in B. napus. PMID:23698630

  1. A gaseous acetic acid treatment to disinfect fenugreek seeds and black pepper inoculated with pathogenic and spoilage bacteria.

    PubMed

    Nei, Daisuke; Enomoto, Katsuyoshi; Nakamura, Nobutaka

    2015-08-01

    Contamination of spices by pathogenic and/or spoilage bacteria can be deleterious to consumer's health and cause deterioration of foods, and inactivation of such bacteria is necessary for the food industry. The present study examined the effect of gaseous acetic acid treatment in reducing Escherichia coli O157:H7, Salmonella Enteritidis and Bacillus subtilis populations inoculated on fenugreek seeds and black pepper. Treatment with gaseous acetic acid at 0.3 mmol/L, 0.6 mmol/L and 4.7 mmol/L for 1-3 h significantly reduced the populations of E. coli O157:H7 and Salmonella Enteritidis on black pepper and fenugreek seeds at 55 °C (p < 0.05). The gas treatments at 4.7 mmol/L were more effective in inactivating the pathogens than the treatment at 0.3 mmol/L. An approximately 5.0 log reduction was obtained after 3 h of treatment with 4.7 mmol/L acetic acid. No significant reductions in the population of B. subtilis spores inoculated on fenugreek seeds and black pepper were obtained after the gas treatments at 0.3 mmol/L or 0.6 mmol/L (p > 0.05). However, the gas treatment at 4.7 mmol/L significantly reduced B. subtilis spores (p < 0.05), and 4.0 log CFU/g and 3.5 log CFU/g reductions on fenugreek seeds and black pepper, respectively, were obtained after 3 h of treatment. PMID:25846935

  2. Physiological and molecular analyses of black and yellow seeded Brassica napus regulated by 5-aminolivulinic acid under chromium stress.

    PubMed

    Gill, Rafaqat A; Ali, Basharat; Islam, Faisal; Farooq, Muhammad A; Gill, Muhammad B; Mwamba, Theodore M; Zhou, Weijun

    2015-09-01

    Brassica napus L. is a promising oilseed crop among the oil producing species. So, it is prime concern to screen the metal tolerant genotypes in order to increase the oilseed rape production through the utilization of pollutant soil regimes. Nowadays, use of plant growth regulators against abiotic stress is one of the major objectives of researchers. In this study, an attempt was carried out to analyze the pivotal role of exogenously applied 5-amenolevulinic acid (ALA) on alleviating chromium (Cr)-toxicity in black and yellow seeded B. napus. Plants of two cultivars (ZS 758 - a black seed type, and Zheda 622 - a yellow seed type) were treated with 400 μM Cr with or without 15 and 30 mg/L ALA. Results showed that exogenously applied ALA improved the plant growth and increased ALA contents; however, it decreased the Cr concentration in B. napus leaves under Cr-toxicity. Moreover, exogenous ALA reduced oxidative stress by up-regulating antioxidant enzyme activities and their related gene expression. Further, results suggested that stress responsive protein's transcript level such as HSP90-1 and MT-1 were increased under Cr stress alone in both cultivars. Exogenously applied ALA further enhanced the expression rate in both genotypes and obviously results were found in favor of cultivar ZS 758. The ultrastructural changes were observed more obvious in yellow seeded than black seeded cultivar; however, exogenously applied ALA helped the plants to recover their cell turgidity under Cr stress. The present study describes a detailed molecular mechanism how ALA regulates the plant growth by improving antioxidant machinery and related transcript levels, cellular modification as well as stress related genes expression under Cr-toxicity. PMID:26079286

  3. Delivery of Flavonoids and Saponins from Black Bean (Phaseolus vulgaris) Seed Coats Incorporated into Whole Wheat Bread

    PubMed Central

    Chávez-Santoscoy, Rocio A.; Lazo-Vélez, Marco A.; Serna-Sáldivar, Sergio O.; Gutiérrez-Uribe, Janet A.

    2016-01-01

    Cereal-based products can be used as vehicles for the delivery of relevant bioactive compounds since they are staple foods for most cultures throughout the world. The health promoting benefits of flavonoids and saponins contained in black bean seed coats have been previously described. In the present work, the effect of adding flavonoids and saponins from black bean seed coat to the typical yeast-leavened whole wheat bread formulation in terms of bread features, organoleptic properties and phytochemical profile was studied. The retention of bioactive compounds was determined and the inhibitory effects of in vitro enzyme digested samples on two colon cancer cell lines (Caco-2 and HT29) was evaluated. The addition of bioactive compounds did not significantly affect baking properties or texture parameters. Among organoleptic properties of enriched breads, only crumb color was affected by the addition of bioactive compounds. However, the use of whole wheat flour partially masked the effect on color. More than 90% of added flavonoids and saponins and 80% of anthocyanins were retained in bread after baking. However, saponins were reduced more than 50% after the in vitro enzyme digestion. The black bean seed coat phytochemicals recovered after in vitro enzyme digestion of enriched breads significantly reduced by 20% the viability of colon cancer cells without affecting standard fibroblast cells (p < 0.05). PMID:26901186

  4. Delivery of Flavonoids and Saponins from Black Bean (Phaseolus vulgaris) Seed Coats Incorporated into Whole Wheat Bread.

    PubMed

    Chávez-Santoscoy, Rocio A; Lazo-Vélez, Marco A; Serna-Sáldivar, Sergio O; Gutiérrez-Uribe, Janet A

    2016-01-01

    Cereal-based products can be used as vehicles for the delivery of relevant bioactive compounds since they are staple foods for most cultures throughout the world. The health promoting benefits of flavonoids and saponins contained in black bean seed coats have been previously described. In the present work, the effect of adding flavonoids and saponins from black bean seed coat to the typical yeast-leavened whole wheat bread formulation in terms of bread features, organoleptic properties and phytochemical profile was studied. The retention of bioactive compounds was determined and the inhibitory effects of in vitro enzyme digested samples on two colon cancer cell lines (Caco-2 and HT29) was evaluated. The addition of bioactive compounds did not significantly affect baking properties or texture parameters. Among organoleptic properties of enriched breads, only crumb color was affected by the addition of bioactive compounds. However, the use of whole wheat flour partially masked the effect on color. More than 90% of added flavonoids and saponins and 80% of anthocyanins were retained in bread after baking. However, saponins were reduced more than 50% after the in vitro enzyme digestion. The black bean seed coat phytochemicals recovered after in vitro enzyme digestion of enriched breads significantly reduced by 20% the viability of colon cancer cells without affecting standard fibroblast cells (p < 0.05). PMID:26901186

  5. Probing the growth of supermassive black holes at z > 6 with LOFAR

    NASA Astrophysics Data System (ADS)

    Rhook, Kirsty J.; Haehnelt, Martin G.

    2006-12-01

    HII regions surrounding supermassive black holes (SMBHs) in an otherwise still neutral intergalactic medium (IGM) are likely to be the most easily detectable sources by future 21-cm experiments like LOFAR. We have made predictions for the size distribution of such HII regions for several physically motivated models for BH growth at high redshift and compared this to the expected LOFAR sensitivity to these sources. The number of potentially detectable HII regions does not only depend on the ionization state of the IGM and the decoupling of the spin temperature of the neutral hydrogen from the cosmic microwave background temperature, but is also strongly sensitive to the rate of growth of BHs at high redshift. If the SMBHs at redshift 6 were built up via continuous Eddington-limited accretion from low mass seed BHs at high redshift, then LOFAR is not expected to detect isolated QSO HII regions at redshifts much larger than 6, and only if the IGM is still significantly neutral. If the high-redshift growth of BHs starts with massive seed BHs and is driven by short-lived accretion events following the merging of BH hosting galaxies then the detection of HII regions surrounding SMBHs may extend to redshifts as large as 8-9 but is still very sensitive to the redshift to which the IGM remains significantly neutral. The most optimistic predictions are for a model where the SMBHs at z > 6 have grown slowly. HII regions around SMBHs may then be detected to significantly larger redshifts.

  6. Probing the disc wind-jet connection in black hole transients

    NASA Astrophysics Data System (ADS)

    Diaz Trigo, Maria

    2010-10-01

    We propose 4 observations of one high inclination black hole low mass X-ray binary (LMXB) at different stages of its outburst. We will investigate the presence of X-ray narrow absorption/emission features in the XMM spectra, which are a signature of a disc wind, and their relation to the accreting regime. Such features, identified with ions like FeXXV and FeXXVI, have been observed in a number of LMXBs and give us information about the mass outflow rate and the launching mechanism of the wind. With simultaneous radio observations we will probe the jet power as a function of the wind properties and how the radio flux density correlates with the X-ray flux at different accretion regimes. We will also investigate the broadening mechanism of the FeK emission line detected up to now in several LMXBs.

  7. Probing the disc wind-jet connection in black hole transients II

    NASA Astrophysics Data System (ADS)

    Diaz Trigo, Maria

    2013-10-01

    We propose six observations of one high inclination black hole low mass X-ray binary (LMXB) at different stages of its outburst. We will investigate the presence of X-ray narrow absorption/emission features in the XMM spectra, which are a signature of a disc wind, and their relation to the accreting regime. Such features, identified with ions like FeXXV and FeXXVI, have been observed in a number of LMXBs and give us information about the mass outflow rate and the launching mechanism of the wind. With simultaneous radio observations we will probe the jet power as a function of the wind properties and how the radio flux density correlates with the X-ray flux at different accretion regimes. We will also investigate the broadening mechanism of the FeK emission line detected up to now in several LMXBs.

  8. Dietary Black Raspberry Seed Oil Ameliorates Inflammatory Activities in db/db Mice.

    PubMed

    Lee, Hee Jae; Jung, Hana; Cho, Hyunnho; Lee, Kiuk; Kwak, Ho-Kyung; Hwang, Keum Taek

    2016-06-01

    The objective of this study was to evaluate the status of the markers related to inflammation in db/db mice fed black raspberry seed (BRS) oil, which is rich in α-linolenic acid. Mice were divided into four groups: (1) C57BL/6 mice fed 16 % calories from soybean oil (normal CON); (2) C57BL/KsJ-db/db mice fed 16 % calories from soybean oil (CON); (3) C57BL/KsJ-db/db mice fed 8 % calories from soybean and 8 % calories from BRS oil (BRS 50 %); and (4) C57BL/KsJ-db/db mice fed 16 % calories from BRS oil (BRS 100 %). After 10 weeks, n-6/n-3 fatty acid ratios were significantly (P < 0.05) lower in the livers and epididymal adipose tissues of the BRS 50 % and BRS 100 % mice than in the CON. Serum TNFα and IL-6 were significantly (P < 0.05) lower in the BRS 50 % and BRS 100 % than in the CON. Serum IL-10 was significantly (P < 0.05) higher in the BRS 100 % than the CON. In the liver and epididymal adipose tissue, mRNA levels of pro-inflammatory markers in the BRS 50 % and BRS 100 % were lower than in the CON. Anti-inflammatory markers were higher in the epididymal adipose tissues of the BRS 50 % and BRS 100 % than in the CON. In the epididymal adipose tissue, macrophage infiltration markers (F4/80 and CD68) and leptin mRNA were significantly (P < 0.05) lower in the BRS 50 % and BRS 100 % than in the CON. Results of this study suggest that BRS oil may have anti-inflammatory effects in obese diabetic mice by ameliorating inflammatory responses. PMID:27165261

  9. Detecting tidal disruption events of massive black holes in normal galaxies with the Einstein Probe

    NASA Astrophysics Data System (ADS)

    Yuan, W.; Komossa, S.; Zhang, C.; Feng, H.; Ling, Z.-X.; Zhao, D. H.; Zhang, S.-N.; Osborne, J. P.; O'Brien, P.; Willingale, R.; Lapington, J.; Lapington

    2016-02-01

    Stars are tidally disrupted and accreted when they approach massive black holes (MBHs) closely, producing a flare of electromagnetic radiation. The majority of the (approximately two dozen) tidal disruption events (TDEs) identified so far have been discovered by their luminous, transient X-ray emission. Once TDEs are detected in much larger numbers, in future dedicated transient surveys, a wealth of new applications will become possible. Here, we present the proposed Einstein Probe mission, which is a dedicated time-domain soft X-ray all-sky monitor aiming at detecting X-ray transients including TDEs in large numbers. The mission consists of a wide-field micro-pore Lobster-eye imager (60° × 60°), and is designed to carry out an all-sky transient survey at energies of 0.5-4 keV. It will also carry a more sensitive telescope for X-ray follow-ups, and will be capable of issuing public transient alerts rapidly. Einstein Probe is expected to revolutionise the field of TDE research by detecting several tens to hundreds of events per year from the early phase of flares, many with long-term, well sampled lightcurves.

  10. Simulating the formation of massive seed black holes in the early Universe - II. Impact of rate coefficient uncertainties

    NASA Astrophysics Data System (ADS)

    Glover, Simon C. O.

    2015-11-01

    We investigate how uncertainties in the chemical and cooling rate coefficients relevant for a metal-free gas influence our ability to determine the critical ultraviolet field strength required to suppress H2 cooling in high-redshift atomic cooling haloes. The suppression of H2 cooling is a necessary prerequisite for the gas to undergo direct collapse and form an intermediate mass black hole. These black holes can then act as seeds for the growth of the supermassive black holes (SMBHs) observed at redshifts z ˜ 6. The viability of this model for SMBH formation depends on the critical ultraviolet field strength, Jcrit: if this is too large, then too few seeds will form to explain the observed number density of SMBHs. We show in this paper that there are five key chemical reactions whose rate coefficients are uncertain enough to significantly affect Jcrit. The most important of these is the collisional ionization of hydrogen by collisions with other hydrogen atoms, as the rate for this process is very poorly constrained at the low energies relevant for direct collapse. The total uncertainty introduced into Jcrit by this and the other four reactions could in the worst case approach a factor of five. We also show that the use of outdated or inappropriate values for the rates of some chemical reactions in previous studies of the direct collapse mechanism may have significantly affected the values of Jcrit determined by these studies.

  11. New Probe of Dark-Matter Properties: Gravitational Waves from an Intermediate-Mass Black Hole Embedded in a Dark-Matter Minispike

    NASA Astrophysics Data System (ADS)

    Eda, Kazunari; Itoh, Yousuke; Kuroyanagi, Sachiko; Silk, Joseph

    2013-05-01

    An intermediate-mass black hole (IMBH) may have a dark-matter (DM) minihalo around it and develop a spiky structure within less than a parsec from the IMBH. When a stellar mass object is captured by the minihalo, it eventually infalls into such an IMBH due to gravitational wave backreaction which in turn could be observed directly by future space-borne gravitational wave experiments such as eLISA and NGO. In this Letter, we show that the gravitational wave (GW) detectability strongly depends on the radial profile of the DM distribution. So if the GW is detected, the power index, that is, the DM density distribution, would be determined very accurately. The DM density distribution obtained would make it clear how the IMBH has evolved from a seed black hole and whether the IMBH has experienced major mergers in the past. Unlike the γ-ray observations of DM annihilation, GW is just sensitive to the radial profile of the DM distribution and even to noninteracting DM. Hence, the effect we demonstrate here can be used as a new and powerful probe into DM properties.

  12. New probe of dark-matter properties: gravitational waves from an intermediate-mass black hole embedded in a dark-matter minispike.

    PubMed

    Eda, Kazunari; Itoh, Yousuke; Kuroyanagi, Sachiko; Silk, Joseph

    2013-05-31

    An intermediate-mass black hole (IMBH) may have a dark-matter (DM) minihalo around it and develop a spiky structure within less than a parsec from the IMBH. When a stellar mass object is captured by the minihalo, it eventually infalls into such an IMBH due to gravitational wave backreaction which in turn could be observed directly by future space-borne gravitational wave experiments such as eLISA and NGO. In this Letter, we show that the gravitational wave (GW) detectability strongly depends on the radial profile of the DM distribution. So if the GW is detected, the power index, that is, the DM density distribution, would be determined very accurately. The DM density distribution obtained would make it clear how the IMBH has evolved from a seed black hole and whether the IMBH has experienced major mergers in the past. Unlike the γ-ray observations of DM annihilation, GW is just sensitive to the radial profile of the DM distribution and even to noninteracting DM. Hence, the effect we demonstrate here can be used as a new and powerful probe into DM properties. PMID:23767709

  13. Antioxidant Activity and Hepatoprotective Potential of Black Seed, Honey and Silymarin on Experimental Liver Injuries Induced by CCl4 in Rats

    NASA Astrophysics Data System (ADS)

    Khadr, Mona E.; Mahdy, Karam A.; El-Shamy, Karima A.; Morsy, Fatma A.; El-Zayat, Salwa R.; Abd-Allah, Azza A.

    The possible antioxidant activity and hepatoprotective potential of black seed honey and silymarin on CCl4 induced liver injuries in rats was investigated. Fifty male rats were used in this study and divided into five groups, 10 rats each. Group 1 served as a control; group 2 injected 1 mL kg-1 day-1 CCl4 intraperitoneally 3 times a week for 4 week, groups 3, 4 and 5 subjected to the same injection of CCl4 and co-treatment with black seed, honey and silymarin (50 mg kg-1 b.wt.), respectively, daily by stomach tube for 4 weeks. Blood and tissue samples were taken for biochemical and histopathological studies. The results revealed that CCl4 administration caused significant elevations in the levels of MDA, NO, MMP-2, AST and ALT. Histopathological observations showed severe damage in the liver. Its fibrotic areas were measured using Image Analyzer. Combined treatment with CCl4 and black seed, honey and silymarin showed marked improvement in antioxidant status and in histopathological findings as well as reductions in the fibrotic areas. These results concluded that black seed, honey and silymarin have protective characteristics against CCl4-induced rat liver injury through potentiation of antioxidant capacity of liver cells and prevention of oxidative stress that accompanied with CCl4 hepatotoxicity. The protective effect was higher in silymarin followed by black seed then honey.

  14. Electron density measurement of cesium seeded negative ion source by surface wave probe

    SciTech Connect

    Kisaki, M.; Tsumori, K.; Nakano, H.; Ikeda, K.; Osakabe, M.; Nagaoka, K.; Shibuya, M.; Sato, M.; Sekiguchi, H.; Komada, S.; Kondo, T.; Hayashi, H.; Asano, E.; Takeiri, Y.; Kaneko, O.

    2012-02-15

    Electron density measurements of a large-scaled negative ion source were carried out with a surface wave probe. By comparison of the electron densities determined with the surface wave probe and a Langmuir probe, it was confirmed that the surface wave probe is highly available for diagnostic of the electron density in H{sup -} ion sources. In addition, it was found that the ratio of the electron density to the H{sup -} ion density dramatically decreases with increase of a bias voltage and the H{sup -} ions become dominant negative particles at the bias voltage of more than 6 V.

  15. Inoculation Preparation Affects Survival of Salmonella enterica on Whole Black Peppercorns and Cumin Seeds Stored at Low Water Activity.

    PubMed

    Bowman, Lauren S; Waterman, Kim M; Williams, Robert C; Ponder, Monica A

    2015-07-01

    Salmonellosis has been increasingly associated with contaminated spices. Identifying inoculation and stabilization methods for Salmonella on whole spices is important for development of validated inactivation processes. The objective of this study was to examine the effects of inoculation preparation on the recoverability of Salmonella enterica from dried whole peppercorns and cumin seeds. Whole black peppercorns and cumin seeds were inoculated with S. enterica using one dry transfer method and various wet inoculation methods: immersion of spice seeds in tryptic soy broth (TSB) plus Salmonella for 24 h (likely leading to inclusion of Salmonella in native microbiota biofilms formed around the seeds), application of cells grown in TSB, and/or application of cells scraped from tryptic soy agar (TSA). Postinoculation seeds were dried to a water activity of 0.3 within 24 h and held for 28 days. Seeds were sampled after drying (time 0) and periodically during the 28 days of storage. Salmonella cells were enumerated by serial dilution and plated onto xylose lysine Tergitol (XLT4) agar and TSA. Recovery of Salmonella was high after 28 days of storage but was dependent on inoculation method, with 4.05 to 6.22 and 3.75 to 8.38 log CFU/g recovered from peppercorns and cumin seeds, respectively, on XLT4 agar. The changes in surviving Salmonella (log CFU per gram) from initial inoculation levels after 28 days were significantly smaller for the biofilm inclusion method (+0.142pepper, +0.186cumin) than for the other inoculation methods (-0.425pepper, -2.029cumin for cells grown on TSA; -0.641pepper, -0.718cumin for dry transfer; -1.998pepper for cells grown in TSB). In most cases, trends for reductions of total aerobic bacteria were similar to those of Salmonella. The inoculation method influenced the recoverability of Salmonella from whole peppercorns and cumin seeds after drying. The most stable inoculum strategies were dry transfer, 24-h incubation of Salmonella and spices in

  16. Primordial black holes as a novel probe of primordial gravitational waves. II. Detailed analysis

    NASA Astrophysics Data System (ADS)

    Nakama, Tomohiro; Suyama, Teruaki

    2016-08-01

    Recently we have proposed a novel method to probe primordial gravitational waves from upper bounds on the abundance of primordial black holes (PBHs). When the amplitude of primordial tensor perturbations generated in the early Universe is fairly large, they induce substantial scalar perturbations due to their second-order effects. If these induced scalar perturbations are too large when they reenter the horizon, then PBHs are overproduced, their abundance exceeding observational upper limits. That is, primordial tensor perturbations on superhorizon scales can be constrained from the absence of PBHs. In our recent paper we have only shown simple estimations of these new constraints, and hence in this paper, we present detailed derivations, solving the Einstein equations for scalar perturbations induced at second order in tensor perturbations. We also derive an approximate formula for the probability density function of induced density perturbations, necessary to relate the abundance of PBHs to the primordial tensor power spectrum, assuming primordial tensor perturbations follow Gaussian distributions. Our new upper bounds from PBHs are compared with other existing bounds obtained from big bang nucleosynthesis, cosmic microwave background, LIGO/Virgo and pulsar timing arrays.

  17. Probing the electronic states and impurity effects in black phosphorus vertical heterostructures

    NASA Astrophysics Data System (ADS)

    Chen, Xiaolong; Wang, Lin; Wu, Yingying; Gao, Heng; Wu, Yabei; Qin, Guanhua; Wu, Zefei; Han, Yu; Xu, Shuigang; Han, Tianyi; Ye, Weiguang; Lin, Jiangxiazi; Long, Gen; He, Yuheng; Cai, Yuan; Ren, Wei; Wang, Ning

    2016-03-01

    Atomically thin black phosphorus (BP) is a promising two-dimensional material for fabricating electronic and optoelectronic nano-devices with high mobility and tunable bandgap structures. However, the charge-carrier mobility in few-layer phosphorene (monolayer BP) is mainly limited by the presence of impurity and disorders. In this study, we demonstrate that vertical BP heterostructure devices offer great advantages in probing the electron states of monolayer and few-layer phosphorene at temperatures down to 2 K through capacitance spectroscopy. Electronic states in the conduction and valence bands of phosphorene are accessible over a wide range of temperature and frequency. Exponential band tails have been determined to be related to disorders. Unusual phenomena such as the large temperature-dependence of the electron state population in few-layer phosphorene have been observed and systematically studied. By combining the first-principles calculation, we identified that the thermal excitation of charge trap states and oxidation-induced defect states were the main reasons for this large temperature dependence of the electron state population and degradation of the on-off ratio in phosphorene field-effect transistors.

  18. Prospects for Probing Strong Gravity with a Pulsar-Black Hole System

    NASA Technical Reports Server (NTRS)

    Wex, N.; Liu, K.; Eatough, R. P.; Kramer, M.; Cordes, J. M.; Lazio, T. J. W.

    2012-01-01

    The discovery of a pulsar (PSR) in orbit around a black hole (BH) is expected to provide a superb new probe of relativistic gravity and BH properties. Apart from a precise mass measurement for the BH, one could expect a clean verification of the dragging of space-time caused by the BH spin. In order to measure the quadrupole moment of the BH for testing the no-hair theorem of general relativity (GR), one has to hope for a sufficiently massive BH. In this respect, a PSR orbiting the super-massive BH in the center of our Galaxy would be the ultimate laboratory for gravity tests with PSRs. But even for gravity theories that predict the same properties for BHs as GR, a PSR-BH system would constitute an excellent test system, due to the high grade of asymmetry in the strong field properties of these two components. Here we highlight some of the potential gravity tests that one could expect from different PSR-BH systems.

  19. Probing the spin of the central black hole in the Galactic Centre with secondary images

    NASA Astrophysics Data System (ADS)

    Jørgensen, Jonas Helboe; Bjælde, Ole Eggers; Hannestad, Steen

    2016-06-01

    This paper explores the possibility of determining the spin of the supermassive black hole (SMBH) in Sgr A* by using secondary images of stars orbiting the SMBH. The photons propagate close to the SMBH and their trajectories probe the space-time in a region where the spin of the SMBH is important. We find the appearance of spikes in the secondary image, which depends on the angular momentum and spin axis of the SMBH and study the specific case of the star S2 in detail. The spikes have a magnitude of ˜29 in the K band and the required angular resolution is of the order of 15-20 μas. The combination of these two requirements poses an extreme observational challenge, but might be possible with interferometric observations in the sub-mm regime. The next possible time frame for observing this effect on the star S2 is in the late 2017 and then it repeats with the period of the star.

  20. Near-extremal Black Holes as Initial Conditions of Long GRB Supernovae and Probes of Their Gravitational Wave Emission

    NASA Astrophysics Data System (ADS)

    van Putten, Maurice H. P. M.

    2015-09-01

    Long gamma-ray bursts (GRBs) associated with supernovae and short GRBs with extended emission (SGRBEE) from mergers are probably powered by black holes as a common inner engine, as their prompt GRB emission satisfies the same Amati correlation in the Ep,i-Eiso plane. We introduce modified Bardeen equations to identify hyper-accretion driving newly formed black holes in core-collapse supernovae to near-extremal spin as a precursor to prompt GRB emission. Subsequent spin-down is observed in the BATSE catalog of long GRBs. Spin-down provides a natural unification of long durations associated with the lifetime of black hole spin for normal long GRBs and SGRBEEs, given the absence of major fallback matter in mergers. The results point to major emissions unseen in high frequency gravitational waves. A novel matched filtering method is described for LIGO-Virgo and KAGRA broadband probes of nearby core-collapse supernovae at essentially maximal sensitivity.

  1. The Safety and Pharmacokinetics of Cyanidin-3-Glucoside after 2-Week Administration of Black Bean Seed Coat Extract in Healthy Subjects

    PubMed Central

    Jeon, Sangil; Han, Seunghoon; Lee, Jongtae; Hong, Taegon

    2012-01-01

    We analyzed the pharmacokinetics of C3G on data from twelve subjects, after 2-week multiple dosing of black bean (Phaseolus vulgaris, Cheongjakong-3-ho) seed coat extract, using the mixed effect analysis method (NONMEM, Ver. 6.2), as well as the conventional non-compartmental method. We also examined the safety and tolerability. The PK analysis used plasma concentrations of the C3G on day 1 and 14. There was no observed accumulation of C3G after 2-week multiple dosing of black bean seed coat extract. The typical point estimates of PK were CL (clearance)=3,420 l/h, V (volume)=7,280 L, Ka (absorption constant)=9.94 h-1, ALAG (lag time)=0.217 h. The black bean seed coat extract was well tolerated and there were no serious adverse events. In this study, we confirmed that a significant amount of C3G was absorbed in human after given the black bean seed coat extract. PMID:22915990

  2. The origin of seed photons for Comptonization in the black hole binary Swift J1753.5-0127

    NASA Astrophysics Data System (ADS)

    Kajava, J. J. E.; Veledina, A.; Tsygankov, S.; Neustroev, V.

    2016-06-01

    Aims: The black hole binary Swift J1753.5-0127 is providing a unique data set to study accretion flows. Various investigations of this system and of other black holes have not, however, led to an agreement on the accretion flow geometry or on the seed photon source for Comptonization during different stages of X-ray outbursts. We place constraints on these accretion flow properties by studying long-term spectral variations of this source. Methods: We performed phenomenological and self-consistent broad band spectral modeling of Swift J1753.5-0127 using quasi-simultaneous archived data from INTEGRAL/ISGRI, Swift/UVOT/XRT/BAT, RXTE/PCA/HEXTE, and MAXI/GSC instruments. Results: We identify a critical flux limit, F ~ 1.5 × 10-8 erg cm-2 s-1, and show that the spectral properties of Swift J1753.5-0127 are markedly different above and below this value. Above the limit, during the outburst peak, the hot medium seems to intercept roughly 50 percent of the disk emission. Below it, in the outburst tail, the contribution of the disk photons reduces significantly and the entire spectrum from the optical to X-rays can be produced by a synchrotron-self-Compton mechanism. The long-term variations in the hard X-ray spectra are caused by erratic changes of the electron temperatures in the hot medium. Thermal Comptonization models indicate unreasonably low hot medium optical depths during the short incursions into the soft state after 2010, suggesting that non-thermal electrons produce the Comptonized tail in this state. The soft X-ray excess, likely produced by the accretion disk, shows peculiarly stable temperatures for over an order of magnitude changes in flux. Conclusions: The long-term spectral trends of Swift J1753.5-0127 are likely set by variations of the truncation radius and a formation of a hot, quasi-spherical inner flow in the vicinity of the black hole. In the late outburst stages, at fluxes below the critical limit, the source of seed photons for Comptonization is

  3. Positive or negative? The impact of X-ray feedback on the formation of direct collapse black hole seeds

    NASA Astrophysics Data System (ADS)

    Regan, John A.; Johansson, Peter H.; Wise, John H.

    2016-09-01

    A nearby source of Lyman-Werner (LW) photons is thought to be a central component in dissociating H2 and allowing for the formation of a direct collapse black hole seed. Nearby sources are also expected to produce copious amounts of hydrogen ionizing photons and X-ray photons. We study here the feedback effects of the X-ray photons by including a spectrum due to high-mass X-ray binaries on top of a galaxy with a stellar spectrum. We explicitly trace photon packages emerging from the nearby source and track the radiative and chemical effects of the multifrequency source (Ephoton = 0.76 eV → 7500 eV). We find that X-rays have a strongly negative feedback effect, compared to a stellar only source, when the radiative source is placed at a separation greater than ≳ 1 kpc. The X-rays heat the low and medium density gas in the envelope surrounding the collapsing halo suppressing the mass inflow. The result is a smaller enclosed mass compared to the stellar only case. However, for separations of ≲ 1 kpc, the feedback effects of the X-rays becomes somewhat neutral. The enhanced LW intensity at close separations dissociates more H2 and this gas is heated due to stellar photons alone, the addition of X-rays is then not significant. This distance dependence of X-ray feedback suggests that a Goldilocks zone exists close to a forming galaxy where X-ray photons have a much smaller negative feedback effect and ideal conditions exist for creating massive black hole seeds.

  4. The Direct Collapse of a Massive Black Hole Seed under the Influence of an Anisotropic Lyman-Werner Source

    NASA Astrophysics Data System (ADS)

    Regan, John A.; Johansson, Peter H.; Wise, John H.

    2014-11-01

    The direct collapse model of supermassive black hole seed formation requires that the gas cools predominantly via atomic hydrogen. To this end we simulate the effect of an anisotropic radiation source on the collapse of a halo at high redshift. The radiation source is placed at a distance of 3 kpc (physical) from the collapsing object and is set to emit monochromatically in the center of the Lyman-Werner (LW) band. The LW radiation emitted from the high redshift source is followed self-consistently using ray tracing techniques. Due to self-shielding, a small amount of H2 is able to form at the very center of the collapsing halo even under very strong LW radiation. Furthermore, we find that a radiation source, emitting >1054 (~ 103 J21) photons s-1, is required to cause the collapse of a clump of M ~ 105 M ⊙. The resulting accretion rate onto the collapsing object is ~0.25 M ⊙ yr-1. Our results display significant differences, compared to the isotropic radiation field case, in terms of the H2 fraction at an equivalent radius. These differences will significantly affect the dynamics of the collapse. With the inclusion of a strong anisotropic radiation source, the final mass of the collapsing object is found to be M ~ 105 M ⊙. This is consistent with predictions for the formation of a supermassive star or quasi-star leading to a supermassive black hole.

  5. Proteomic Analysis of the Protein Expression Profile in the Mature Nigella sativa (Black Seed).

    PubMed

    Alanazi, Ibrahim O; Benabdelkamel, Hicham; Alfadda, Assim A; AlYahya, Sami A; Alghamdi, Waleed M; Aljohi, Hasan A; Almalik, Abdulaziz; Masood, Afshan

    2016-08-01

    Nigella sativa (N. sativa) seed has been used as an important nutritional flavoring agent and in traditional medicine for treating many illnesses since ancient times. Understanding the proteomic component of the seed may lead to enhance the understanding of its structural and biological functional complexity. In this study, we have analyzed its proteome profile based on gel-based proteome mapping technique that includes one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry strategy. We have not come across any such studies that have been performed in N. sativa seeds up to date. A total of 277 proteins were identified, and their functional, metabolic, and location-wise annotations were carried out using the UniProt database. The majority of proteins identified in the proteome dataset based on their function were those involved in enzyme catalytic activity, nucleotide binding, and protein binding while the major cellular processes included regulation of biological process followed by regulation of secondary biological process, cell organization and biogenesis, protein metabolism, and transport. The identified proteome was localized mainly to the nucleus then to the cytoplasm, plasma membrane, mitochondria, plastid, and others. A majority of the proteins were involved in biochemical pathways involving carbohydrate metabolism, amino acid and shikimate pathway, lipid metabolism, nucleotide, cell organization and biogenesis, transport, and defense processes. The identified proteins in the dataset help to improve our understanding of the pathways involved in N. sativa seed metabolism and its biochemical features and detail out useful information that may help to utilize these proteins. This study could thus pave a way for future further high-throughput studies using a more targeted proteomic approach. PMID:27020565

  6. Phytochemical screening and evaluation of Monechma ciliatum (black mahlab) seed extracts as antimicrobial agents

    PubMed Central

    Ahmed Oshi, Murtada; Mohmmed Abdelkarim, Abdelkarim

    2013-01-01

    Objective: Tribes in Nubia Mountains regions of Sudan used Monechma ciliatum seeds for common cold and other chest allergic conditions as a traditional medicine. The aim of this paper is to validate this traditional practice scientifically. Materials and Methods: Monechma ciliatum seeds were screened for major phytochemical groups using standard methods. Different extracts were bioassayed in- vitro for their bioactivity to inhibit the growth of pathogenic bacteria and fungi. Results: Phytochemical screening results showed the presence of flavonoids, tannins, triterpens, and anthraquinones. Staphylococcus aureus was found to be sensitive to both water extract with zones of inhibition 22 – 26 mm at concentrations of 50 and 100mg/ml and ethanol extract 17 mm at concentration of 100 mg/ml. The growth of Klebsiella pneumoniae was inhibited by ethanol extract with zones of inhibition equal to 16, 26, and 33 mm at concentrations of 50, 100, and 150 mg/ml, respectively. Pseudomonas aeruginosa was insensitive to all extracts used. Similarly, all used fungi were found to be insensitive to extracts used. The minimum inhibitory concentrations of the extracts against microorganisms were ranged from 12.5 to 25 mg/ml. Conclusion: The findings of the current study support the traditional uses of the plant's seed in the therapy of respiratory tract infections caused by Staphylococcus aureus and Klebsiella pneumoniae. PMID:25050266

  7. Probing the Relationship Between Black Hole Mass and Galaxy Mass for Reverberation-Mapped AGN

    NASA Astrophysics Data System (ADS)

    Ou-Yang, Benjamin; Bentz, Misty; Johnson, Megan C.

    2016-01-01

    We investigate the relationship between the black hole mass and galaxy mass for active galactic nuclei (AGN) with direct black hole mass measurements. Black hole masses were determined from reverberation mapping, which relies on the velocity of the broad line region (BLR) clouds and the light travel time as a measure of the size of the BLR. We constrain the rotation velocity, and therefore the mass, of each AGN host galaxy with HI spectroscopy obtained at the NRAO Green Bank Telescope. We also explore the relationship between black hole mass and dark matter mass by constraining the stellar mass component with ground-based and Hubble Space Telescope optical images combined with the integrated HI flux as a constraint the mass of the gas component. Black hole scaling relations such as these can provide convenient alternatives for large numbers of black hole mass estimates when time and resource constraints preclude black hole mass measurements. Additionally, they can provide constraints for simulations of galaxy evolution and co-evolution with the central black hole.

  8. Probing the magnetic field structure in Sgr A* on Black Hole Horizon Scales with Polarized Radiative Transfer Simulations

    NASA Astrophysics Data System (ADS)

    Gold, Roman; McKinney, Jonathan; Johnson, Michael; Doeleman, Sheperd; Event Horizon Telescope Collaboration

    2016-03-01

    Accreting black holes (BHs) are at the core of relativistic astrophysics as messengers of the strong-field regime of General Relativity and prime targets of several observational campaigns, including imaging the black hole shadow in SagA* and M87 with the Event Horizon Telescope. I will present results from general-relativistic, polarized radiatiative transfer models for the inner accretion flow in Sgr A*. The models use time dependent, global GRMHD simulations of hot accretion flows including standard-and-normal-evolution (SANE) and magnetically arrested disks (MAD). I present comparisons of these synthetic data sets to the most recent observations with the Event Horizon Telescope and show how the data distinguishes the models and probes the magnetic field structure.

  9. Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes

    SciTech Connect

    Jiang, X.; Estes, M.K.; Metcalf, T.G.; Melnick, J.L

    1986-10-01

    The development and trials of a nucleic acid hybridization test for the detection of hepatitis A virus (HAV) in estuarine samples within 48 h are described. Approximately 10/sup 4/ physical particlels of HAV per dot could be detected. Test sensitivity was optimized by the consideration of hydbridization stringency, /sup 32/P energy level, probe concentration, and nucleic acid binding to filters. Test specificity was shown by a lack of cross-hybridization with other enteroviruses and unrelated nucleic acids. Potential false-positive reactions between bacterial DNA in samples and residual vector DNA contamination of purified nucleotide sequences in probes were eliminated by DNase treatment of samples. Humic acid at concentrations of up to 100 mg/liter caused only insignificant decreases in test sensitivity. Interference with hybridization by organic components of virus-containing eluates was removed by proteinase K digestion followed by phenol extraction and ethanol precipitation. The test is suitable for detecting naturally occurring HAV in samples from polluted estuarine environments.

  10. Anticoagulant effect of polyphenols-rich extracts from black chokeberry and grape seeds.

    PubMed

    Bijak, Michał; Bobrowski, Mateusz; Borowiecka, Marta; Podsędek, Anna; Golański, Jacek; Nowak, Paweł

    2011-09-01

    Blood coagulation consists of a series of zymogens that can be converted by limited proteolysis to active enzymes leading to the generation of thrombin. Fresh plasma and human thrombin was incubated with extracts from berries of Aronia melanocarpa or seeds of Vitis vinifera (0.5; 5; 50 μg/ml). The in vitro experiments showed that both extracts prolonged clotting time and decreased the maximal velocity of fibrin polymerization in human plasma. Moreover thrombin incubation with both extracts results in the inhibition of amidolytic activity of this enzyme. It gives hopes for development of diet supplements, which may be preventing thrombosis in pathological states. PMID:21596108

  11. Antithrombin effect of polyphenol-rich extracts from black chokeberry and grape seeds.

    PubMed

    Bijak, Michał; Saluk, Joanna; Ponczek, Michał Błażej; Nowak, Paweł

    2013-01-01

    Thrombin is a serine protease that cleaves the peptide bonds in proteins located on the carboxyl side of arginine. Thrombin plays a central role in thromboembolic diseases, which are the major cause of mortality. The aim of the study was to estimate the effects of plant extracts on proteolytic properties of thrombin. Thrombin was incubated with polyphenol-rich extracts from berries of Aronia melanocarpa or seeds of Vitis vinifera (0.5, 5, 50 µg/mL) and with polyphenols ((+)-catechin, (-)-epicatechin, gallic acid, chlorogenic acid, procyanidin B1, cyanidin, cyanidin 3-glucoside, quercetin). The in vitro experiments showed that both extracts in all used concentrations inhibited proteolytic activity of thrombin observed as inhibition of thrombin-induced fibrinogen polymerization, stabilized fibrin formation, and platelet aggregation. Moreover, thrombin amidolytic activity was inhibited by polyphenols belonging to the flavonoid class. Results presented in this study indicate that polyphenol-rich extracts from berries of A. melanocarpa and seeds of V. vinifera may become promising dietary supplements in the prevention of thrombotic states. PMID:22473647

  12. Two-colour pump–probe experiments with a twin-pulse-seed extreme ultraviolet free-electron laser

    PubMed Central

    Allaria, E.; Bencivenga, F.; Borghes, R.; Capotondi, F.; Castronovo, D.; Charalambous, P.; Cinquegrana, P.; Danailov, M. B.; De Ninno, G.; Demidovich, A.; Di Mitri, S.; Diviacco, B.; Fausti, D.; Fawley, W. M.; Ferrari, E.; Froehlich, L.; Gauthier, D.; Gessini, A.; Giannessi, L.; Ivanov, R.; Kiskinova, M.; Kurdi, G.; Mahieu, B.; Mahne, N.; Nikolov, I.; Masciovecchio, C.; Pedersoli, E.; Penco, G.; Raimondi, L.; Serpico, C.; Sigalotti, P.; Spampinati, S.; Spezzani, C.; Svetina, C.; Trovò, M.; Zangrando, M.

    2013-01-01

    Exploring the dynamics of matter driven to extreme non-equilibrium states by an intense ultrashort X-ray pulse is becoming reality, thanks to the advent of free-electron laser technology that allows development of different schemes for probing the response at variable time delay with a second pulse. Here we report the generation of two-colour extreme ultraviolet pulses of controlled wavelengths, intensity and timing by seeding of high-gain harmonic generation free-electron laser with multiple independent laser pulses. The potential of this new scheme is demonstrated by the time evolution of a titanium-grating diffraction pattern, tuning the two coherent pulses to the titanium M-resonance and varying their intensities. This reveals that an intense pulse induces abrupt pattern changes on a time scale shorter than hydrodynamic expansion and ablation. This result exemplifies the essential capabilities of the jitter-free multiple-colour free-electron laser pulse sequences to study evolving states of matter with element sensitivity. PMID:24048228

  13. The direct collapse of a massive black hole seed under the influence of an anisotropic Lyman-Werner source

    SciTech Connect

    Regan, John A.; Johansson, Peter H.; Wise, John H.

    2014-11-10

    The direct collapse model of supermassive black hole seed formation requires that the gas cools predominantly via atomic hydrogen. To this end we simulate the effect of an anisotropic radiation source on the collapse of a halo at high redshift. The radiation source is placed at a distance of 3 kpc (physical) from the collapsing object and is set to emit monochromatically in the center of the Lyman-Werner (LW) band. The LW radiation emitted from the high redshift source is followed self-consistently using ray tracing techniques. Due to self-shielding, a small amount of H{sub 2} is able to form at the very center of the collapsing halo even under very strong LW radiation. Furthermore, we find that a radiation source, emitting >10{sup 54} (∼ 10{sup 3} J{sub 21}) photons s{sup –1}, is required to cause the collapse of a clump of M ∼ 10{sup 5} M {sub ☉}. The resulting accretion rate onto the collapsing object is ∼0.25 M {sub ☉} yr{sup –1}. Our results display significant differences, compared to the isotropic radiation field case, in terms of the H{sub 2} fraction at an equivalent radius. These differences will significantly affect the dynamics of the collapse. With the inclusion of a strong anisotropic radiation source, the final mass of the collapsing object is found to be M ∼ 10{sup 5} M {sub ☉}. This is consistent with predictions for the formation of a supermassive star or quasi-star leading to a supermassive black hole.

  14. Differential protection of black-seed oil on econucleotidase, cholinesterases and aminergic catabolizing enzyme in haloperidol-induced neuronal damage of male rats

    PubMed Central

    Akintunde, Jacob K.; Irechukwu, C. Abigail

    2016-01-01

    Background: The antipsychotic, haloperidol, is extremely efficient in the treatment of schizophrenia but its application is constrained because of irreversible adverse drug reactions. Hence, in this study, we investigate the differential effects of black seed oil on cholinesterase [acetylcholinesterase (AChE) and butrylcholinesterase (BuChE), ectonucleotidase (5′-nucleotidase), lactate dehydrogenase (LDH) and monoamine oxidase (MAO)] activities and relevant markers of oxidative stress in the cerebrum of haloperidol-induced neuronal-damaged rats. Methods: The animals were divided into six groups (n = 10): normal control rats; haloperidol-induced rats: induced rats were pre-, co- and post-treated with black-seed oil respectively, while the last group was treated with extract oil only. The treatment was performed via oral administration and the experiment lasted 14 days. Results: The results revealed an increase in 5I nucleotidase, a marker of adenosine triphosphate (ATP) and adenosine monophosphate (AMP) hydrolysis, as well as AChE, BuChE and MAO activities, with concomitant decrease in LDH activity of cerebrum in induced rats when compared with controls. Also, administration of haloperidol caused systemic oxidative damage and adverse histopathological changes in neuronal cells, indications of mental disorder. The differential treatments with black-seed oil prevented these alterations by increasing LDH and decreasing 5I nucleotidase, AChE, BuChE and MAO activities in the cerebrum. Essential oil post-treatment is most efficacious in reversing haloperidol-induced neuronal damage in rat; followed by pre- and cotreatment, respectively. Conclusions: We concluded that essential black-seed oil enhanced the wellness of aminergic, purinergic and cholinergic neurotransmissions of haloperidol-induced neuronal damage in rats. PMID:27493717

  15. Characterization of an acidic chitinase from seeds of black soybean (Glycine max (L) Merr Tainan No. 3).

    PubMed

    Chang, Ya-Min; Chen, Li-Chun; Wang, Hsin-Yi; Chiang, Chui-Liang; Chang, Chen-Tien; Chung, Yun-Chin

    2014-01-01

    Using 4-methylumbelliferyl-β-D-N,N',N″-triacetylchitotrioside (4-MU-GlcNAc3) as a substrate, an acidic chitinase was purified from seeds of black soybean (Glycine max Tainan no. 3) by ammonium sulfate fractionation and three successive steps of column chromatography. The purified chitinase was a monomeric enzyme with molecular mass of 20.1 kDa and isoelectric point of 4.34. The enzyme catalyzed the hydrolysis of synthetic substrates p-nitrophenyl N-acetyl chitooligosaccharides with chain length from 3 to 5 (GlcNAcn, n = 3-5), and pNp-GlcNAc4 was the most degradable substrate. Using pNp-GlcNAc4 as a substrate, the optimal pH for the enzyme reaction was 4.0; kinetic parameters Km and kcat were 245 µM and 10.31 min-1, respectively. This enzyme also showed activity toward CM-chitin-RBV, a polymer form of chitin, and N-acetyl chitooligosaccharides, an oligomer form of chitin. The smallest oligomer substrate was an N-acetylglucosamine tetramer. These results suggested that this enzyme was an endo-splitting chitinase with short substrate cleavage activity and useful for biotechnological applications, in particular for the production of N-acetyl chitooligosaccharides. PMID:25437446

  16. Effect of Allelochemicals from Leaf Leachates of Gmelina arborea on Inhibition of Some Essential Seed Germination Enzymes in Green Gram, Red Gram, Black Gram, and Chickpea

    PubMed Central

    Madhan Shankar, Ramakrishnan; Veeralakshmi, Shanmugham; Rajendran, Ramasamy

    2014-01-01

    The present work focused on identification of allelochemicals from the leaf leachates of Gmelina arborea and analyzing its influence on the germination of red gram, green gram, black gram, and chickpea in terms of the levels of some important germination enzymes like acid phosphatase, catalase, peroxidase, and amylase. The study showed that allelopathic effects were more predominant in chickpea with 80% followed by red gram, green gram, and black gram where the inhibition ranged between 26% and 75%. The vigor index in the seed lots is also considerably reduced. Total chlorophyll content was also reduced by allelopathic effect in all treated seeds ranging between 0.7 and 7.5 μg/g dry weight. The effect of allelochemicals drastically reduced the relative water content in red gram followed by the other seed lots. The total protein content varied considerably in the control and the treated seed lots. Allelochemicals inhibited the expression and activity of the enzymes required for efficient germination. The present study also threw limelight on the effective use of this tree, wherein planting this tree amidst pulse related herb plantations could affect the growth of the economically viable plants, but this tree can very well adapt to diversified soil conditions and rainfall zones. PMID:27350959

  17. Needs of Seeds

    ERIC Educational Resources Information Center

    Keeley, Page

    2011-01-01

    The "Needs of Seeds" formative assessment probe can be used to find out whether students recognize that seeds have needs both similar to and different from plants and other living organisms (Keeley, Eberle, and Tugel 2007). The probe reveals whether students overgeneralize the needs of seeds by assuming they have the same needs as the adult plants…

  18. Antiviral Effects of Black Raspberry (Rubus coreanus) Seed and Its Gallic Acid against Influenza Virus Infection

    PubMed Central

    Lee, Ji-Hye; Oh, Mi; Seok, Jong Hyeon; Kim, Sella; Lee, Dan Bi; Bae, Garam; Bae, Hae-In; Bae, Seon Young; Hong, Young-Min; Kwon, Sang-Oh; Lee, Dong-Hun; Song, Chang-Seon; Mun, Ji Young; Chung, Mi Sook; Kim, Kyung Hyun

    2016-01-01

    Influenza is a serious public health concern worldwide, as it causes significant morbidity and mortality. The emergence of drug-resistant viral strains requires new approaches for the treatment of influenza. In this study, Rubus coreanus seed (RCS) that is left over from the production of wine or juice was found to show antiviral activities against influenza type A and B viruses. Using the time-of-addition plaque assay, viral replication was almost completely abolished by simultaneous treatment with the RCS fraction of less than a 1-kDa molecular weight (RCSF1). One of the polyphenols derived from RCSF1, gallic acid (GA), identified by liquid chromatography-tandem mass spectrometry, showed inhibitory effects against both influenza type A and B viruses, albeit at relatively high concentrations. RCSF1 was bound to hemagglutinin protein, inhibited hemagglutination significantly and disrupted viral particles, whereas GA was found to only disrupt the viral particles by using transmission electron microscopy. In BALB/c mice infected with influenza virus, oral administration of RCSF1 significantly improved the survival rate and reduced the viral titers in the lungs. Our results demonstrate that RCSF1 and GA show potent and broad antiviral activity against influenza A and B type viruses and are promising sources of agents that target virus particles. PMID:27275830

  19. Antiviral Effects of Black Raspberry (Rubus coreanus) Seed and Its Gallic Acid against Influenza Virus Infection.

    PubMed

    Lee, Ji-Hye; Oh, Mi; Seok, Jong Hyeon; Kim, Sella; Lee, Dan Bi; Bae, Garam; Bae, Hae-In; Bae, Seon Young; Hong, Young-Min; Kwon, Sang-Oh; Lee, Dong-Hun; Song, Chang-Seon; Mun, Ji Young; Chung, Mi Sook; Kim, Kyung Hyun

    2016-01-01

    Influenza is a serious public health concern worldwide, as it causes significant morbidity and mortality. The emergence of drug-resistant viral strains requires new approaches for the treatment of influenza. In this study, Rubus coreanus seed (RCS) that is left over from the production of wine or juice was found to show antiviral activities against influenza type A and B viruses. Using the time-of-addition plaque assay, viral replication was almost completely abolished by simultaneous treatment with the RCS fraction of less than a 1-kDa molecular weight (RCSF1). One of the polyphenols derived from RCSF1, gallic acid (GA), identified by liquid chromatography-tandem mass spectrometry, showed inhibitory effects against both influenza type A and B viruses, albeit at relatively high concentrations. RCSF1 was bound to hemagglutinin protein, inhibited hemagglutination significantly and disrupted viral particles, whereas GA was found to only disrupt the viral particles by using transmission electron microscopy. In BALB/c mice infected with influenza virus, oral administration of RCSF1 significantly improved the survival rate and reduced the viral titers in the lungs. Our results demonstrate that RCSF1 and GA show potent and broad antiviral activity against influenza A and B type viruses and are promising sources of agents that target virus particles. PMID:27275830

  20. Forming supermassive black hole seeds under the influence of a nearby anisotropic multifrequency source

    NASA Astrophysics Data System (ADS)

    Regan, John A.; Johansson, Peter H.; Wise, John H.

    2016-07-01

    The photodissociation of H2 by a nearby anisotropic source of radiation is seen as a critical component in creating an environment in which a direct collapse black hole may form. Employing radiative transfer we model the effect of multifrequency (0.76-60 eV) radiation on a collapsing halo at high redshift. We vary both the shape of the spectrum which emits the radiation and the distance to the emitting galaxy. We use blackbody spectra with temperatures of T = 104 K and 105 K and a realistic stellar spectrum. We find that an optimal zone exists between 1 and 4 kpc from the emitting galaxy. If the halo resides too close to the emitting galaxy the photoionizing radiation creates a large H II region which effectively disrupts the collapsing halo, too far from the source and the radiation flux drops below the level of the expected background and the H2 fraction remains too high. When the emitting galaxy is initially placed between 1 and 2 kpc from the collapsing halo, with a spectral shape consistent with a star-forming high-redshift galaxy, then a large central core forms. The mass of the central core is between 5000 and 10 000 M⊙ at a temperature of approximately 1000 K. This core is however surrounded by a reservoir of hotter gas at approximately 8000 K, which leads to mass inflow rates of the order of ˜0.1 M⊙ yr-1.

  1. Direct collapse to supermassive black hole seeds: comparing the AMR and SPH approaches

    PubMed Central

    Luo, Yang; Nagamine, Kentaro; Shlosman, Isaac

    2016-01-01

    We provide detailed comparison between the adaptive mesh refinement (AMR) code enzo-2.4 and the smoothed particle hydrodynamics (SPH)/N-body code gadget-3 in the context of isolated or cosmological direct baryonic collapse within dark matter (DM) haloes to form supermassive black holes. Gas flow is examined by following evolution of basic parameters of accretion flows. Both codes show an overall agreement in the general features of the collapse; however, many subtle differences exist. For isolated models, the codes increase their spatial and mass resolutions at different pace, which leads to substantially earlier collapse in SPH than in AMR cases due to higher gravitational resolution in gadget-3. In cosmological runs, the AMR develops a slightly higher baryonic resolution than SPH during halo growth via cold accretion permeated by mergers. Still, both codes agree in the build-up of DM and baryonic structures. However, with the onset of collapse, this difference in mass and spatial resolution is amplified, so evolution of SPH models begins to lag behind. Such a delay can have effect on formation/destruction rate of H2 due to UV background, and on basic properties of host haloes. Finally, isolated non-cosmological models in spinning haloes, with spin parameter λ ∼ 0.01–0.07, show delayed collapse for greater λ, but pace of this increase is faster for AMR. Within our simulation set-up, gadget-3 requires significantly larger computational resources than enzo-2.4 during collapse, and needs similar resources, during the pre-collapse, cosmological structure formation phase. Yet it benefits from substantially higher gravitational force and hydrodynamic resolutions, except at the end of collapse. PMID:27279786

  2. Direct collapse to supermassive black hole seeds: comparing the AMR and SPH approaches

    NASA Astrophysics Data System (ADS)

    Luo, Yang; Nagamine, Kentaro; Shlosman, Isaac

    2016-07-01

    We provide detailed comparison between the adaptive mesh refinement (AMR) code ENZO-2.4 and the smoothed particle hydrodynamics (SPH)/N-body code GADGET-3 in the context of isolated or cosmological direct baryonic collapse within dark matter (DM) haloes to form supermassive black holes. Gas flow is examined by following evolution of basic parameters of accretion flows. Both codes show an overall agreement in the general features of the collapse; however, many subtle differences exist. For isolated models, the codes increase their spatial and mass resolutions at different pace, which leads to substantially earlier collapse in SPH than in AMR cases due to higher gravitational resolution in GADGET-3. In cosmological runs, the AMR develops a slightly higher baryonic resolution than SPH during halo growth via cold accretion permeated by mergers. Still, both codes agree in the build-up of DM and baryonic structures. However, with the onset of collapse, this difference in mass and spatial resolution is amplified, so evolution of SPH models begins to lag behind. Such a delay can have effect on formation/destruction rate of H2 due to UV background, and on basic properties of host haloes. Finally, isolated non-cosmological models in spinning haloes, with spin parameter λ ˜ 0.01-0.07, show delayed collapse for greater λ, but pace of this increase is faster for AMR. Within our simulation set-up, GADGET-3 requires significantly larger computational resources than ENZO-2.4 during collapse, and needs similar resources, during the pre-collapse, cosmological structure formation phase. Yet it benefits from substantially higher gravitational force and hydrodynamic resolutions, except at the end of collapse.

  3. Forming supermassive black hole seeds under the influence of a nearby anisotropic multifrequency source

    PubMed Central

    Regan, John A.; Johansson, Peter H.; Wise, John H.

    2016-01-01

    The photodissociation of H2 by a nearby anisotropic source of radiation is seen as a critical component in creating an environment in which a direct collapse black hole may form. Employing radiative transfer we model the effect of multifrequency (0.76–60 eV) radiation on a collapsing halo at high redshift. We vary both the shape of the spectrum which emits the radiation and the distance to the emitting galaxy. We use blackbody spectra with temperatures of T = 104 K and 105 K and a realistic stellar spectrum. We find that an optimal zone exists between 1 and 4 kpc from the emitting galaxy. If the halo resides too close to the emitting galaxy the photoionizing radiation creates a large H ii region which effectively disrupts the collapsing halo, too far from the source and the radiation flux drops below the level of the expected background and the H2 fraction remains too high. When the emitting galaxy is initially placed between 1 and 2 kpc from the collapsing halo, with a spectral shape consistent with a star-forming high-redshift galaxy, then a large central core forms. The mass of the central core is between 5000 and 10 000 M⊙ at a temperature of approximately 1000 K. This core is however surrounded by a reservoir of hotter gas at approximately 8000 K, which leads to mass inflow rates of the order of ∼0.1 M⊙ yr−1. PMID:27279787

  4. Direct Collapse to Supermassive Black Hole Seeds: Comparing the AMR and SPH Approaches

    NASA Astrophysics Data System (ADS)

    Luo, Yang; Nagamine, Kentaro; Shlosman, Isaac

    2016-03-01

    We provide detailed comparison between the AMR code Enzo-2.4 and the SPH/N-body code GADGET-3 in the context of isolated or cosmological direct baryonic collapse within dark matter (DM) halos to form supermassive black holes. Gas flow is examined by following evolution of basic parameters of accretion flows. Both codes show an overall agreement in the general features of the collapse, however, many subtle differences exist. For isolated models, the codes increase their spatial and mass resolutions at different pace, which leads to substantially earlier collapse in SPH than in AMR cases due to higher gravitational resolution in GADGET-3. In cosmological runs, the AMR develops a slightly higher baryonic resolution than SPH during halo growth via cold accretion permeated by mergers. Still, both codes agree in the buildup of DM and baryonic structures. However, with the onset of collapse, this difference in mass and spatial resolution is amplified, so evolution of SPH models begins to lag behind. Such a delay can have effect on formation/destruction rate of H2 due to UV background, and on basic properties of host halos. Finally, isolated non-cosmological models in spinning halos, with spin parameter λ ˜ 0.01 - 0.07, show delayed collapse for greater λ, but pace of this increase is faster for AMR. Within our simulation setup, GADGET-3 requires significantly larger computational resources than Enzo-2.4 during collapse, and needs similar resources, during the pre-collapse, cosmological structure formation phase. Yet it benefits from substantially higher gravitational force and hydrodynamic resolutions, except at the end of collapse.

  5. Chemical Characteristics of Cold-Pressed Blackberry, Black Raspberry, and Blueberry Seed Oils and the Role of the Minor Components in Their Oxidative Stability.

    PubMed

    Li, Quanquan; Wang, Jiankang; Shahidi, Fereidoon

    2016-07-01

    The chemical characteristics of cold-pressed blackberry, black raspberry, and blueberry seed oils were evaluated for their fatty acid composition, positional distribution of fatty acids, triacylglycerol (TAG) profile, and minor component profile. The role of minor components, including tocols and pigments, on the oxidative stability was also investigated using high-temperature- and fluorescent-lighting-induced oxidation before and after tested berry seed oils were stripped of their minor components. The results indicated that all tested berry seed oils contained significant levels of palmitic (C16:0), stearic (C18:0), oleic (18:1), linoleic (C18:2ω-6), and α-linolenic (C18:3ω-3) acids, along with a favorable ratio of ω-6/ω-3 fatty acids (1.49-3.86); palmitic, stearic, oleic, and α-linolenic acids were predominantly distributed on the terminal positions. Six TAGs, namely, LnLnLn, LnLLn, LLLn, LLL, OLL, and OLLn, were the major species detected in the tested berry seed oils. Total tocol contents were 286.3-1302.9 mg/kg, which include α-, γ-, and δ-tocopherols as well as δ-tocotrienol. Oxidative stability of the three berry seed oils was compromised after the removal of tocols under high-temperature-induced oxidation, while the loss of pigments (chlorophylls) led to weak oxidative stability when exposed to fluorescent lights. PMID:27203814

  6. Transient Probes of Accretion Physics, Jet Formation, and Black Hole Demographic

    NASA Astrophysics Data System (ADS)

    Gezari, Suvi

    2012-10-01

    Theorists first surmised that massive black holes (MBHs) will inevitably tidally disrupt and consume stars in their vicinity, and that the resulting flare of radiation could be used as a signpost for a dormant MBH lurking otherwise undetectable in the center of a galaxy. In the last decade, major progress has been made in the discovery of candidates using surveys across the electromagnetic spectrum, from the X-rays to the optical. We propose for TOO XMM-Newton X-ray imaging of TDE candidates from the Pan- STARRS1 Medium Deep Survey in order to characterize the prompt X-ray properties of optically-selected candidates for the first time.

  7. Production of maize tortillas and cookies from nixtamalized flour enriched with anthocyanins, flavonoids and saponins extracted from black bean (Phaseolus vulgaris) seed coats.

    PubMed

    Chávez-Santoscoy, Rocio A; Gutiérrez-Uribe, Janet A; Serna-Saldivar, Sergio O; Perez-Carrillo, Esther

    2016-02-01

    Ethanolic extract from black beans coat is a source of flavonoids, saponins and antocyanins. Nixtamalized maize flours (NF) are used for the preparation of products such as tortillas, tortillas chips, cookies among others. The objective of this research was to study the effect on textural parameters and color after adding flavonoids, saponins and anthocyanins from black bean seed coat in NF used for the production of tortillas and gluten-free cookies. Furthermore, the retention of bioactive compounds after tortilla and gluten-free-cookie preparation was assessed. Ethanolic extracts of black bean seed coats were added (3g/kg or 7 g/kg) to NF in order to prepare corn tortillas and gluten free cookies characterized in terms of dimensions, color and texture. Addition of 7 g/kg affected the color of cookies and tortillas without effect on texture and dimensions. It was possible to retain more than 80% and 60% of bioactives into baked tortillas and cookies, respectively. PMID:26304324

  8. Rapid variability as a probe of warped space-time around accreting black holes

    NASA Astrophysics Data System (ADS)

    Axelsson, Magnus

    2016-07-01

    The geometry of the inner accretion flow of X-ray binaries is complex, with multiple regions contributing to the observed emission. Frequency-resolved spectroscopy is a powerful tool in breaking this spectral degeneracy. We have extracted the spectra of the strong low-frequency quasi-periodic oscillation (QPO) and its harmonic in GX339-4 and XTE J1550-564, and compare these to the time-averaged spectrum and the spectrum of the rapid (<0.1 s) variability. Our results support the picture where the QPO arises from vertical (Lense-Thirring) precession of an inhomogeneous hot flow, softer at larger radii closer to the truncated disc and harder in the innermost parts where the rapid variability is produced. This coupling between variability and spectra allows us to constrain the soft Comptonization component, breaking the degeneracy plaguing the time-averaged spectrum and revealing the geometry of the accretion flow close to the black hole.

  9. Probing the active massive black hole candidate in the center of NGC 404 with VLBI

    SciTech Connect

    Paragi, Z.; Frey, S.; Kaaret, P.; Cseh, D.; Kharb, P.

    2014-08-10

    Recently, Nyland et al. argued that the radio emission observed in the center of the dwarf galaxy NGC 404 originates in a low-luminosity active galactic nucleus powered by a massive black hole (MBH, M ≲ 10{sup 6} M{sub ☉}). High-resolution radio detections of MBHs are rare. Here we present sensitive, contemporaneous Chandra X-ray, and very long baseline interferometry (VLBI) radio observations with the European VLBI Network. The source is detected in the X-rays, and shows no long-term variability. If the hard X-ray source is powered by accretion, the apparent low accretion efficiency would be consistent with a black hole (BH) in the hard state. Hard state BHs are known to show radio emission compact on the milliarcsecond scales. However, the central region of NGC 404 is resolved out on 10 mas (0.15-1.5 pc) scales. Our VLBI non-detection of a compact, partially self-absorbed radio core in NGC 404 implies that either the BH mass is smaller than 3{sub −2}{sup +5}×10{sup 5} M{sub ☉}, or the source does not follow the fundamental plane of BH activity relation. An alternative explanation is that the central BH is not in the hard state. The radio emission observed on arcsecond (tens of parsecs) scales may originate in nuclear star formation or extended emission due to AGN activity, although the latter would not be typical considering the structural properties of low-ionization nuclear emission-line region galaxies with confirmed nuclear activity.

  10. A jet emission model to probe the dynamics of accretion and ejection coupling in black hole X-ray binaries

    NASA Astrophysics Data System (ADS)

    Malzac, Julien

    2016-07-01

    Compact jets are probably the most common form of jets in X-ray binaries and Active Galactic Nuclei. They seem to be present in all sources in the so-called hard X-ray spectral state. They are characterised by a nearly flat Spectral Energy Distribution (SED) extending from the radio to the infrared bands. This emission is usually interpreted as partially self absorbed synchrotron emission from relativistic leptons accelerated in the jet. The observed flat spectral shape requires energy dissipation and acceleration of particules over a wide range of distances along the jet. This distributed energy dissipation is likely to be powered by internal shocks caused by fluctuations of the outflow velocity. I will discuss such an internal shock model in the context of black hole binaries. I will show that internal shocks can produce the observed SEDs and also predict a strong, wavelength dependent, variability that resembles the observed one. The assumed velocity fluctuations of the jet must originate in the accretion flow. The model thus predicts a strong connection between the observable properties of the jet in the radio to IR bands, and the variability of the accretion flow as observed in X-rays. If the model is correct, this offers a unique possibility to probe the dynamics of the coupled accretion and ejection processes leading to the formation of compact jets.

  11. De novo Assembly, Characterization of Immature Seed Transcriptome and Development of Genic-SSR Markers in Black Gram [Vigna mungo (L.) Hepper

    PubMed Central

    Souframanien, J.; Reddy, Kandali Sreenivasulu

    2015-01-01

    Black gram [V. mungo (L.) Hepper] is an important legume crop extensively grown in south and south-east Asia, where it is a major source of dietary protein for its predominantly vegetarian population. However, lack of genomic information and markers has become a limitation for genetic improvement of this crop. Here, we report the transcriptome sequencing of the immature seeds of black gram cv. TU94-2, by Illumina paired end sequencing technology to generate transcriptome sequences for gene discovery and genic-SSR marker development. A total of 17.2 million paired-end reads were generated and 48,291 transcript contigs (TCS) were assembled with an average length of 443 bp. Based on sequence similarity search, 33,766 TCS showed significant similarity to known proteins. Among these, only 29,564 TCS were annotated with gene ontology (GO) functional categories. A total number of 138 unique KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways were identified, of which majority of TCS are grouped into purine metabolism (678) followed by pyrimidine metabolism (263). A total of 48,291 TCS were searched for SSRs and 1,840 SSRs were identified in 1,572 TCS with an average frequency of one SSR per 11.9 kb. The tri-nucleotide repeats were most abundant (35%) followed by di-nucleotide repeats (32%). PCR primer pairs were successfully designed for 933 SSR loci. Sequences analyses indicate that about 64.4% and 35.6% of the SSR motifs were present in the coding sequences (CDS) and untranslated regions (UTRs) respectively. Tri-nucleotide repeats (57.3%) were preferentially present in the CDS. The rate of successful amplification and polymorphism were investigated using selected primers among 18 black gram accessions. Genic-SSR markers developed from the Illumina paired end sequencing of black gram immature seed transcriptome will provide a valuable resource for genetic diversity, evolution, linkage mapping, comparative genomics and marker-assisted selection in black gram. PMID

  12. Sowing the seeds of massive black holes in small galaxies: Young clusters as the building blocks of ultracompact dwarf galaxies

    SciTech Connect

    Amaro-Seoane, Pau; Konstantinidis, Symeon; Freitag, Marc Dewi; Coleman Miller, M.; Rasio, Frederic A. E-mail: simos@ari.uni-heidelberg.de E-mail: miller@astro.umd.edu

    2014-02-20

    Interacting galaxies often have complexes of hundreds of young stellar clusters of individual masses ∼10{sup 4}-10{sup 6} M {sub ☉} in regions that are a few hundred parsecs across. These cluster complexes interact dynamically, and their coalescence is a candidate for the origin of some ultracompact dwarf galaxies. Individual clusters with short relaxation times are candidates for the production of intermediate-mass black holes of a few hundred solar masses, via runaway stellar collisions prior to the first supernovae in a cluster. It is therefore possible that a cluster complex hosts multiple intermediate-mass black holes that may be ejected from their individual clusters due to mergers or binary processes, but bound to the complex as a whole. Here we explore the dynamical interaction between initially free-flying massive black holes and clusters in an evolving cluster complex. We find that, after hitting some clusters, it is plausible that the massive black hole will be captured in an ultracompact dwarf forming near the center of the complex. In the process, the hole typically triggers electromagnetic flares via stellar disruptions, and is also likely to be a prominent source of gravitational radiation for the advanced ground-based detectors LIGO and VIRGO. We also discuss other implications of this scenario, notably that the central black hole could be considerably larger than expected in other formation scenarios for ultracompact dwarfs.

  13. Comparative Studies on Polyphenolic Composition, Antioxidant and Diuretic Effects of Nigella sativa L. (Black Cumin) and Nigella damascena L. (Lady-in-a-Mist) Seeds.

    PubMed

    Toma, Claudia-Crina; Olah, Neli-Kinga; Vlase, Laurian; Mogoșan, Cristina; Mocan, Andrei

    2015-01-01

    This study was performed to evaluate the phenolic profile, antioxidant and diuretic effects of black cumin and lady-in-a-mist seeds. In the phenolic profile, differences between the two species are significant. Qualitative and quantitative analyses of the phenolic compounds were performed using a HPLC-UV/MS method. Hyperoside was the only identified flavonoid glycoside (1.08 ± 0.01 μg∙g-1 dw plant material), in the N. damascena extract. Regarding the flavonol profile, kaempferol was identified before the hydrolysis, only in the N. sativa extract (6.06 ± 0.02 μg∙g-1 dw plant material) and quercetin only in N. damascena seeds (14.35 ± 0.02 μg∙g-1 dw plant material). The antioxidant potential of the two species was tested through several electron transfer assays, which indicated, excepting for the FRAP assay, N. damascena as exhibiting a higher free radical scavenging activity. The diuretic activity of the two extracts was tested using a rat-experimental model on acute diuresis. Administration of the ethanolic extract of N. sativa (100 mg∙kg-1) resulted in a significant increase in urine volume, although less than found with the reference drug; in addition N. damascena extract did not present a diuretic effect. In reference to the elimination of Na+, K+ and uric acid, the black cumin extract exhibited a higher natriuretic than kaluretic effect and a similar uricosuric effect with control and N. damascena. For N. damascena, the Na+/K+ ratio was sub unitary, but not due to an increasing of the kaluretic effect, but mostly to a decrease of Na+ excretion. PMID:26016547

  14. Seeds in Flight

    ERIC Educational Resources Information Center

    Martin, Willard K.

    1978-01-01

    Discussed are the seed dispersal mechanisms of six different plants: big-leaf maple, pincushion tree, tree of heaven, squirting cucumber, digger pine, and bull thistle. Elaborate color and black-and-white drawings illustrate the text. (MA)

  15. Herbal infusions of black seed and wheat germ oil: Their chemical profiles, in vitro bio-investigations and effective formulations as Phyto-Nanoemulsions.

    PubMed

    Gumus, Z Pinar; Guler, Emine; Demir, Bilal; Barlas, F Baris; Yavuz, Murat; Colpankan, Dilara; Senisik, A Murat; Teksoz, Serap; Unak, Perihan; Coskunol, Hakan; Timur, Suna

    2015-09-01

    The reported studies related to black seed oil (BSO) and wheat germ oil (WGO) have illustrated that they have a wide range of biological activities. Therefore, enhancing the amount of bio-active compounds that caused higher cell based anti-oxidative effect as well as cell proliferation, etc. in seed oils, infusion of crude plant material has been gained importance as a traditional technique. Herein, we accomplished the infusion of Calendula flowers that also contains many phyto-constituents into BSO and WGO. After the infusion of oils, the change of phytochemical amount was investigated and evaluated according to the oils by chromatography, radical scavenging activity. Subsequently, for investigating the biological impact upon live cells, cytotoxicity, cell-based antioxidant capacity, wound healing and radioprotective activity were tested with monkey kidney fibroblast like cells (Vero) and HaCaT keratinocytes. In vitro cell based experiments (wound healing and radioprotective activity) confirmed that Calendula infused BSO and WGO have greater bio-activity when compared to those plain forms. The herbal oils prepared with an effective extraction technique were incorporated into nanoemulsion systems which will be then called as 'Phyto-Nanoemulsion'. After herbal oil biomolecules were encapsulated into nanoemulsion based delivery systems, the designed formulations were investigated in terms of biological activities. In conclusion, these preparations could be a good candidate as a part of dermal cosmetic products or food supplements which have the therapeutic efficiency, especially after radio- or chemotherapy. PMID:26087391

  16. Magnitude Differences in Bioactive Compounds, Chemical Functional Groups, Fatty Acid Profiles, Nutrient Degradation and Digestion, Molecular Structure, and Metabolic Characteristics of Protein in Newly Developed Yellow-Seeded and Black-Seeded Canola Lines.

    PubMed

    Theodoridou, Katerina; Zhang, Xuewei; Vail, Sally; Yu, Peiqiang

    2015-06-10

    Recently, new lines of yellow-seeded (CS-Y) and black-seeded canola (CS-B) have been developed with chemical and structural alteration through modern breeding technology. However, no systematic study was found on the bioactive compounds, chemical functional groups, fatty acid profiles, inherent structure, nutrient degradation and absorption, or metabolic characteristics between the newly developed yellow- and black-seeded canola lines. This study aimed to systematically characterize chemical, structural, and nutritional features in these canola lines. The parameters accessed include bioactive compounds and antinutrition factors, chemical functional groups, detailed chemical and nutrient profiles, energy value, nutrient fractions, protein structure, degradation kinetics, intestinal digestion, true intestinal protein supply, and feed milk value. The results showed that the CS-Y line was lower (P ≤ 0.05) in neutral detergent fiber (122 vs 154 g/kg DM), acid detergent fiber (61 vs 99 g/kg DM), lignin (58 vs 77 g/kg DM), nonprotein nitrogen (56 vs 68 g/kg DM), and acid detergent insoluble protein (11 vs 35 g/kg DM) than the CS-B line. There was no difference in fatty acid profiles except C20:1 eicosenoic acid content (omega-9) which was in lower in the CS-Y line (P < 0.05) compared to the CS-B line. The glucosinolate compounds differed (P < 0.05) in terms of 4-pentenyl, phenylethyl, 3-CH3-indolyl, and 3-butenyl glucosinolates (2.9 vs 1.0 μmol/g) between the CS-Y and CS-B lines. For bioactive compounds, total polyphenols tended to be different (6.3 vs 7.2 g/kg DM), but there were no differences in erucic acid and condensed tannins with averages of 0.3 and 3.1 g/kg DM, respectively. When protein was portioned into five subfractions, significant differences were found in PA, PB1 (65 vs 79 g/kg CP), PB2, and PC fractions (10 vs 33 g/kg CP), indicating protein degradation and supply to small intestine differed between two new lines. In terms of protein structure spectral

  17. Composition, In Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained from Black Cumin Seeds (Nigella sativa L.)

    PubMed Central

    Singh, Sunita; Das, S. S.; Singh, G.; Schuff, Carola; de Lampasona, Marina P.; Catalán, César A. N.

    2014-01-01

    Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%), α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as 11.47 ± 0.05, 10.88 ± 0.9, 9.68 ± 0.06, and 8.33 ± 0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition against Fusarium moniliforme in inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria. PMID:24689064

  18. Comparison of germination and seed bank dynamics of dimorphic seeds of the cold desert halophyte Suaeda corniculata subsp. mongolica

    PubMed Central

    Cao, Dechang; Baskin, Carol C.; Baskin, Jerry M.; Yang, Fan; Huang, Zhenying

    2012-01-01

    Background and Aims Differences in dormancy and germination requirements have been documented in heteromorphic seeds of many species, but it is unknown how this difference contributes to maintenance and regeneration of populations. The primary aim of this study was to compare the seed bank dynamics, including dormancy cycling, of the two seed morphs (black and brown) of the cold desert halophyte Suaeda corniculata and, if differences were found, to determine their influence on regeneration of the species. Method Seeds of the two seed morphs were buried, exhumed and tested monthly for 24 months over a range of temperatures and salinities, and germination recovery and viability were determined after exposure to salinity and water stress. Seedling emergence and dynamics of the soil seed bank were also investigated for the two morphs. Key Results Black seeds had an annual dormancy/non-dormancy cycle, while brown seeds, which were non-dormant at maturity, remained non-dormant. Black seeds also exhibited an annual cycle in sensitivity of germination to salinity. Seedlings derived from black seeds emerged in July and August and those from brown seeds in May. Seedlings were recruited from 2·6 % of the black seeds and from 2·8 % of the brown seeds in the soil, and only 0·5 % and 0·4 % of the total number of black and brown seeds in the soil, respectively, gave rise to seedlings that survived to produce seeds. Salinity and water stress induced dormancy in black seeds and decreased viability of brown seeds. Brown seeds formed only a transient soil seed bank and black seeds a persistent seed bank. Conclusions The presence of a dormancy cycle in black but not in brown seeds of S. corniculata and differences in germination requirements of the two morphs cause them to differ in their germination dynamics. The study contributes to our limited knowledge of dormancy cycling and seed bank formation in species producing heteromorphic seeds. PMID:22975287

  19. Inheritance of seed color in Capsicum.

    PubMed

    Zewdie, Y; Bosland, P W

    2003-01-01

    The mode of seed color inheritance in Capsicum was studied via an interspecific hybridization between C. pubescens Ruiz and Pav. (black seed color) and C. eximium Hunz. (yellow seed color). Black seed color was dominant over yellow seed color. The F(2) segregation pattern showed continuous variation. The generation means analysis indicated the presence of a significant effect of additive [d], dominance [h], and additive x additive [i] interaction for seed color inheritance. The estimate for a minimum number of effective factors (genes) involved in seed color inheritance was approximately 3. PMID:12920108

  20. Effects of black pepper (piper nigrum), turmeric powder (curcuma longa) and coriander seeds (coriandrum sativum) and their combinations as feed additives on growth performance, carcass traits, some blood parameters and humoral immune response of broiler chickens.

    PubMed

    Abou-Elkhair, R; Ahmed, H A; Selim, S

    2014-06-01

    Different herbs and spices have been used as feed additives for various purposes in poultry production. This study was conducted to assess the effect of feed supplemented with black pepper (Piper nigrum), turmeric powder (Curcuma longa), coriander seeds (Coriandrum sativum) and their combinations on the performance of broilers. A total of 210 (Cobb) one-d-old chicks were divided into seven groups of 30 birds each. The treatments were: a control group received no supplement, 0.5% black pepper (T1), 0.5% turmeric powder (T2), 2% coriander seeds (T3), a mixture of 0.5% black pepper and 0.5% turmeric powder (T4), a mixture of 0.5% black pepper and 2% coriander seed (T5), and a mixture of 0.5% black pepper, 0.5% turmeric powder and 2% coriander seeds (T6). Higher significant values of body weight gain during the whole period of 5 weeks (p<0.001) were observed in broilers on T1, T3, T5, and T6 compared to control. Dietary supplements with T1, T2, T3, and T6 improved the cumulative G:F of broilers during the whole period of 5 weeks (p<0.001) compared with control. The dressing percentage and edible giblets were not influenced by dietary supplements, while higher values of relative weight of the liver (p<0.05) were obtained in T5 and T6 compared to control. The addition of feed supplements in T5 and T6 significantly increased serum total protein and decreased serum glucose, triglycerides and alkaline phosphatase concentrations compared with the control group (p<0.05). Broilers on T6 showed significant decrease in the serum glutamate pyruvate transaminase concentration (p<0.05) compared to control. The broilers having T5 and T6 supplemented feed had relatively greater antibody titre (p<0.001) at 35 d of age than control. It is concluded that dietary supplements with black pepper or coriander seeds or their combinations enhanced the performance and health status of broiler chickens. PMID:25050023

  1. Thymoquinone, a bioactive component of black caraway seeds, causes G1 phase cell cycle arrest and apoptosis in triple-negative breast cancer cells with mutant p53.

    PubMed

    Sutton, Kimberly M; Greenshields, Anna L; Hoskin, David W

    2014-01-01

    Thymoquinone (TQ) from black caraway seeds has several anticancer activities; however, its effect on triple-negative breast cancer (TNBC) cells that lack functional tumor suppressor p53 is not known. Here, we explored the growth inhibitory effect of TQ on 2 TNBC cell lines with mutant p53. Cell metabolism assays showed that TQ inhibited TNBC cell growth without affecting normal cell growth. Flow cytometric analyses of TQ-treated TNBC cells showed G1 phase cell cycle arrest and apoptosis characterized by the loss of mitochondrial membrane integrity. Western blots of lysates from TQ-treated TNBC cells showed cytochrome c and apoptosis-inducing factor in the cytoplasm, as well as caspase-9 activation consistent with the mitochondrial pathway of apoptosis. Caspase-8 was also activated in TQ-treated TNBC cells, although the mechanism of activation is not clear at this time. Importantly, TQ-induced apoptosis was only partially inhibited by zVAD-fmk, indicating a role for caspase-independent effector molecules. Poly(ADP-ribose) polymerase cleavage and increased γH2AX, as well as reduced Akt phosphorylation and decreased expression of X-linked inhibitor of apoptosis, were evident in TQ-treated cells. Finally, TQ enhanced cisplatin- and docetaxel-induced cytotoxicity. These findings suggest that TQ could be useful in the management of TNBC, even when functional p53 is absent. PMID:24579801

  2. Therapeutic Implications of Black Seed and Its Constituent Thymoquinone in the Prevention of Cancer through Inactivation and Activation of Molecular Pathways

    PubMed Central

    Rahmani, Arshad H.; Alzohairy, Mohammad A.; Khan, Masood A.; Aly, Salah M.

    2014-01-01

    The cancer is probably the most dreaded disease in both men and women and also major health problem worldwide. Despite its high prevalence, the exact molecular mechanisms of the development and progression are not fully understood. The current chemotherapy/radiotherapy regime used to treat cancer shows adverse side effect and may alter gene functions. Natural products are generally safe, effective, and less expensive substitutes of anticancer chemotherapeutics. Based on previous studies of their potential therapeutic uses, Nigella sativa and its constituents may be proved as good therapeutic options in the prevention of cancer. Black seeds are used as staple food in the Middle Eastern Countries for thousands of years and also in the treatment of diseases. Earlier studies have shown that N. sativa and its constituent thymoquinone (TQ) have important roles in the prevention and treatment of cancer by modulating cell signaling pathways. In this review, we summarize the role of N. sativa and its constituents TQ in the prevention of cancer through the activation or inactivation of molecular cell signaling pathways. PMID:24959190

  3. Probing the anisotropic behaviors of black phosphorus by transmission electron microscopy, angular-dependent Raman spectra, and electronic transport measurements

    NASA Astrophysics Data System (ADS)

    Lu, Wanglin; Ma, Xiaomeng; Fei, Zhen; Zhou, Jianguang; Zhang, Zhiyong; Jin, Chuanhong; Zhang, Ze

    2015-07-01

    In this study, we correlated the angular dependence of the Raman response of black phosphorus to its crystallographic orientation by using transmission electron microscopy and Raman spectroscopy. It was found that the intensity of the Ag 2 mode reached a maximum when the polarization direction of the incident light was parallel to the zigzag crystallographic orientation. Notably, it was further confirmed that the zigzag crystallographic direction exhibited superior conductance and carrier mobility. Because of the lattice extension along the armchair direction, an intensification of the anisotropic Raman response was observed. This work provides direct evidence of the correlation between anisotropic properties and crystallographic direction and represents a turning point in the discussion of the angular-dependent electronic properties of black phosphorus.

  4. Jet drifts and flips in radio galaxies as probes of the historical evolution of spin axis in supermassive black holes

    NASA Astrophysics Data System (ADS)

    Saripalli, Lakshmi; Subrahmanyan, Ravi; Hall Roberts, David

    2015-08-01

    Jets in radio galaxies create twin lobes of synchrotron plasma on opposite sides of the host elliptical. The jets are believed to emerge along the spin axis of the central supermassive black hole. The history of evolution in spin axis is traced in the off axis distortions in the radio structure. We have analyzed the radio structures in a large sample of distorted radio galaxies to examine black hole spin axis behavior. These sources are selected specifically to have low axial-ratio structures and hence off-axis distortions that are, however, unbiased with respect to the nature of the distortions.We have imaged 52 radio galaxies having length to width ratio less than 1 to obtain detailed radio structures that enable a tracing of the origin of the off-axis radio emission. The unique sample consists of radio sources where the off axis radio emission originates from strategic locations - regions closer to the host galaxy and from the outer ends of the jets. A third category consists of sources where there is only a swathe of radio emission nearly orthogonal to the radio axis and passing through the central radio core.Our study has highlighted the potential of radio galaxies in tracing black hole spin axis changes over time; we use the occurrence rates of the different categories of sources to derive occurrence rates of drifts and flips in black hole axis. Since the host galaxies are an unbiased sampling of luminous elliptical galaxies, the rates derived are relevant to this parent population (Roberts, Cohen, Lu, Saripalli and Subrahmanyan, 2015, arXiv150203954; Roberts, Saripalli, Subrahmanyan, 2015, arXiv150302021).

  5. Probing the origin of the iron Kα line around stellar and supermassive black holes using X-ray polarimetry

    NASA Astrophysics Data System (ADS)

    Marin, F.; Tamborra, F.

    2014-10-01

    Asymmetric, broad iron lines are a common feature in the X-ray spectra of both X-ray binaries (XRBs) and type-1 Active Galactic Nuclei (AGN). It was suggested that the distortion of the Fe Kα emission results from Doppler and relativistic effects affecting the radiative transfer close to the strong gravitational well of the central compact object: a stellar mass black hole (BH) or neutron star (NS) in the case of XRBs, or a super massive black hole (SMBH) in the case of AGN. However, alternative approaches based on reprocessing and transmission of radiation through surrounding media also attempt to explain the line broadening. So far, spectroscopic and timing analyzes have not yet convinced the whole community to discriminate between the two scenarios. Here we study to which extent X-ray polarimetric measurements of black hole X-ray binaries (BHXRBs) and type-1 AGN could help to identify the possible origin of the line distortion. To do so, we report on recent simulations obtained for the two BH flavors and show that the proposed scenarios are found to behave differently in polarization degree and polarization angle. A relativistic origin for the distortion is found to be more probable in the context of BHXRBs, supporting the idea that the same mechanism should lead the way also for AGN. We show that the discriminating polarization signal could have been detectable by several X-ray polarimetry missions proposed in the past.

  6. Forming Super-Massive Black Hole Seeds under the Influence of a Nearby Anisotropic Multi-Frequency Source

    NASA Astrophysics Data System (ADS)

    Regan, John A.; Johansson, Peter H.; Wise, John H.

    2016-04-01

    The photo-dissociation of {H_2} by a nearby anisotropic source of radiation is seen as a critical component in creating an environment in which a direct collapse black hole may form. Employing radiative transfer we model the effect of multi-frequency (0.76 eV - 60 eV) radiation on a collapsing halo at high redshift. We vary both the shape of the spectrum which emits the radiation and the distance to the emitting galaxy. We use blackbody spectra with temperatures of {T = 10^4 K} and {T = 10^5 K} and a realistic stellar spectrum. We find that an optimal zone exists between 1 kpc and 4 kpc from the emitting galaxy. If the halo resides too close to the emitting galaxy the photo-ionising radiation creates a large HII region which effectively disrupts the collapsing halo, too far from the source and the radiation flux drops below the level of the expected background and the {H_2} fraction remains too high. When the emitting galaxy is initially placed between 1 kpc and 2 kpc from the collapsing halo, with a spectral shape consistent with a star-forming high redshift galaxy, then a large central core forms. The mass of the central core is between 5000 and 10000 M_{⊙}at a temperature of approximately 1000 K. This core is however surrounded by a reservoir of hotter gas at approximately 8000 K which leads to mass inflow rates of the order of ˜0.1 M_{⊙} yr-1.

  7. Proton micro-probe analysis of framboidal pyrite and associated maceral types in a Devonian black shale

    SciTech Connect

    Graham, U.M.; Robl, T.L. . Center for Applied Energy Research); Robertson, J.D. . Dept. of Chemistry)

    1992-01-01

    Framboids are spherical, raspberry-like aggregates of pyrite which are typically associated with organic matter in black shales. Because framboids are often intimately intergrown with macerals of the kerogen in black shales the objectives of this study were to: (1) Select tramboidal pyrite with close spatial relationship to distinct maceral types; (2) Determine the trace-element variations within different maceral types and that of framboidal pyrite occurring adjacent to those macerals and; (3) Examine whether the S/Fe ratios of the tramboids vary based on different maceral-type association. This study investigates a Devonian-Mississippian black shale from East-Central Kentucky. The organic-rich matrix consists predominantly of bituminite, alginite and to lesser extent of vitrinite. Most framboids range between < 1[mu]m and 27 [mu]m in size and typically occur as clusters which are engulfed by lamellar flowing vitrinite, indicating that the framboids were already present before compaction. 161 PIXE-analyses were performed in both macerals and framboids. To understand the likelihood of framboid precursors in macerals the authors checked the constancy of the S, Fe and trace-element content in the immediate vicinity of the framboid particle. Moreover, the authors analyzed traverses through framboids associated with the three different maceral types. The S/Fe ratio of the framboids is always that of stoichiometric pyrite. The combined results suggest that the framboids may have formed independent of the sulfur and trace-element concentration among the macerals. Globular, partly translucent grains were observed to have great resemblances in size and trace-element contents compared to those of framboids. The S/Fe ratio of these grains was typically well in excess of 2.0 suggesting that the transparent matrix may have been a sulfur-rich phase that possibly serves as precursor for the framboids.

  8. Regular black hole remnants and graviatoms with de Sitter interior as heavy dark matter candidates probing inhomogeneity of early universe

    NASA Astrophysics Data System (ADS)

    Dymnikova, Irina; Khlopov, Maxim

    2015-07-01

    We address the question of regular primordial black holes with de Sitter interior, their remnants and gravitational vacuum solitons G-lumps as heavy dark matter candidates providing signatures for inhomogeneity of early universe, which is severely constrained by the condition that the contribution of these objects in the modern density does not exceed the total density of dark matter. Primordial black holes and their remnants seem to be most elusive among dark matter candidates. However, we reveal a nontrivial property of compact objects with de Sitter interior to induce proton decay or decay of neutrons in neutron stars. The point is that they can form graviatoms, binding electrically charged particles. Their observational signatures as dark matter candidates provide also signatures for inhomogeneity of the early universe. In graviatoms, the cross-section of the induced proton decay is strongly enhanced, what provides the possibility of their experimental searches. We predict proton decay paths induced by graviatoms in the matter as an observational signature for heavy dark matter searches at the IceCUBE experiment.

  9. Thermal and pressure stability of myrosinase enzymes from black mustard (Brassica nigra L. W.D.J. Koch. var. nigra), brown mustard (Brassica juncea L. Czern. var. juncea) and yellow mustard (Sinapsis alba L. subsp. maire) seeds.

    PubMed

    Okunade, Olukayode Adediran; Ghawi, Sameer Khalil; Methven, Lisa; Niranjan, Keshavan

    2015-11-15

    This study investigates the effects of temperature and pressure on inactivation of myrosinase extracted from black, brown and yellow mustard seeds. Brown mustard had higher myrosinase activity (2.75 un/mL) than black (1.50 un/mL) and yellow mustard (0.63 un/mL). The extent of enzyme inactivation increased with pressure (600-800 MPa) and temperature (30-70° C) for all the mustard seeds. However, at combinations of lower pressures (200-400 MPa) and high temperatures (60-80 °C), there was less inactivation. For example, application of 300 MPa and 70 °C for 10 min retained 20%, 80% and 65% activity in yellow, black and brown mustard, respectively, whereas the corresponding activity retentions when applying only heat (70° C, 10 min) were 0%, 59% and 35%. Thus, application of moderate pressures (200-400 MPa) can potentially be used to retain myrosinase activity needed for subsequent glucosinolate hydrolysis. PMID:25977054

  10. Probing Out-of-Plane Charge Transport in Black Phosphorus with Graphene-Contacted Vertical Field-Effect Transistors

    NASA Astrophysics Data System (ADS)

    Kang, Junmo; Jariwala, Deep; Ryder, Christopher R.; Wells, Spencer A.; Choi, Yongsuk; Hwang, Euyheon; Cho, Jeong Ho; Marks, Tobin J.; Hersam, Mark C.

    2016-04-01

    Black phosphorus (BP) has recently emerged as a promising narrow band gap layered semiconductor with optoelectronic properties that bridge the gap between semi-metallic graphene and wide band gap transition metal dichalcogenides such as MoS2. To date, BP field-effect transistors have utilized a lateral geometry with in-plane transport dominating device characteristics. In contrast, we present here a vertical field-effect transistor geometry based on a graphene/BP van der Waals heterostructure. The resulting device characteristics include high on-state current densities (> 1600 A/cm2) and current on/off ratios exceeding 800 at low temperature. Two distinct charge transport mechanisms are identified, which are dominant for different regimes of temperature and gate voltage. In particular, the Schottky barrier between graphene and BP determines charge transport at high temperatures and positive gate voltages, whereas tunneling dominates at low temperatures and negative gate voltages. These results elucidate out-of-plane electronic transport in BP, and thus have implications for the design and operation of BP-based van der Waals heterostructures.

  11. Probing Out-of-Plane Charge Transport in Black Phosphorus with Graphene-Contacted Vertical Field-Effect Transistors.

    PubMed

    Kang, Junmo; Jariwala, Deep; Ryder, Christopher R; Wells, Spencer A; Choi, Yongsuk; Hwang, Euyheon; Cho, Jeong Ho; Marks, Tobin J; Hersam, Mark C

    2016-04-13

    Black phosphorus (BP) has recently emerged as a promising narrow band gap layered semiconductor with optoelectronic properties that bridge the gap between semimetallic graphene and wide band gap transition metal dichalcogenides such as MoS2. To date, BP field-effect transistors have utilized a lateral geometry with in-plane transport dominating device characteristics. In contrast, we present here a vertical field-effect transistor geometry based on a graphene/BP van der Waals heterostructure. The resulting device characteristics include high on-state current densities (>1600 A/cm(2)) and current on/off ratios exceeding 800 at low temperature. Two distinct charge transport mechanisms are identified, which are dominant for different regimes of temperature and gate voltage. In particular, the Schottky barrier between graphene and BP determines charge transport at high temperatures and positive gate voltages, whereas tunneling dominates at low temperatures and negative gate voltages. These results elucidate out-of-plane electronic transport in BP and thus have implications for the design and operation of BP-based van der Waals heterostructures. PMID:26950174

  12. Seed dimorphism, nutrients and salinity differentially affect seed traits of the desert halophyte Suaeda aralocaspica via multiple maternal effects

    PubMed Central

    2012-01-01

    Background Maternal effects may influence a range of seed traits simultaneously and are likely to be context-dependent. Disentangling the interactions of plant phenotype and growth environment on various seed traits is important for understanding regeneration and establishment of species in natural environments. Here, we used the seed-dimorphic plant Suaeda aralocaspica to test the hypothesis that seed traits are regulated by multiple maternal effects. Results Plants grown from brown seeds had a higher brown:black seed ratio than plants from black seeds, and germination percentage of brown seeds was higher than that of black seeds under all conditions tested. However, the coefficient of variation (CV) for size of black seeds was higher than that of brown seeds. Seeds had the smallest CV at low nutrient and high salinity for plants from brown seeds and at low nutrient and low salinity for plants from black seeds. Low levels of nutrients increased size and germinability of black seeds but did not change the seed morph ratio or size and germinability of brown seeds. High levels of salinity decreased seed size but did not change the seed morph ratio. Seeds from high-salinity maternal plants had a higher germination percentage regardless of level of germination salinity. Conclusions Our study supports the multiple maternal effects hypothesis. Seed dimorphism, nutrient and salinity interacted in determining a range of seed traits of S. aralocaspica via bet-hedging and anticipatory maternal effects. This study highlights the importance of examining different maternal factors and various offspring traits in studies that estimate maternal effects on regeneration. PMID:23006315

  13. Seed Germination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Initiation of seed germination is a critical decision for plants. It is important for seed populations under natural conditions to spread the timing of germination of individual seeds to maximize the probability of species survival. Therefore, seeds have evolved the multiple layers of mechanisms tha...

  14. Canning Quality and Color Retention in Black Beans

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Black beans are Michigan’s most important dry bean seed type. There is a strong demand for Michigan grown black beans domestically and internationally. Black bean processing presents unique challenges because of the nature of black bean color. Atypical of other bean market classes, black beans are ...

  15. In vivo photoacoustic imaging of prostate brachytherapy seeds

    NASA Astrophysics Data System (ADS)

    Lediju Bell, Muyinatu A.; Kuo, Nathanael P.; Song, Danny Y.; Kang, Jin; Boctor, Emad M.

    2014-03-01

    We conducted an approved canine study to investigate the in vivo feasibility of photoacoustic imaging for intraoperative updates to brachytherapy treatment plans. Brachytherapy seeds coated with black ink were inserted into the canine prostate using methods similar to a human procedure. A transperineal, interstitial, fiber optic light delivery method, coupled to a 1064 nm laser, was utilized to irradiate the prostate and the resulting acoustic waves were detected with a transrectal ultrasound probe. The fiber was inserted into a high dose rate (HDR) brachytherapy needle that acted as a light-diffusing sheath, enabling radial light delivery from the tip of the fiber inside the sheath. The axis of the fiber was located at a distance of 4-9 mm from the long axis of the cylindrical seeds. Ultrasound images acquired with the transrectal probe and post-operative CT images of the implanted seeds were analyzed to confirm seed locations. In vivo limitations with insufficient light delivery within the ANSI laser safety limit (100 mJ/cm2) were overcome by utilizing a short-lag spatial coherence (SLSC) beamformer, which provided average seed contrasts of 20-30 dB for energy densities ranging 8-84 mJ/cm2. The average contrast was improved by up to 20 dB with SLSC beamforming compared to conventional delay-and-sum beamforming. There was excellent agreement between photoacoustic, ultrasound, and CT images. Challenges included visualization of photoacoustic artifacts that corresponded with locations of the optical fiber and hyperechoic tissue structures.

  16. Imagine the Universe! The Anatomy of Black Holes. Probing the Structure & Evolution of the Cosmos. An Information and Activity Booklet. Grades 9-12, 1998-1999.

    ERIC Educational Resources Information Center

    Whitlock, Laura A.; Granger, Kara C.; Mahon, Jane D.

    The information provided in this booklet is meant to give the necessary background information so that the science of black holes can be taught confidently to secondary students. The featured activities can be used to engage and excite students about the topic of black holes in different disciplines and in a number of ways. Activities include: (1)…

  17. Exploring Black Hole Dynamics

    NASA Astrophysics Data System (ADS)

    Chung, Hyeyoun

    2015-10-01

    This thesis explores the evolution of different types of black holes, and the ways in which black hole dynamics can be used to answer questions about other physical systems. We first investigate the differences in observable gravitational effects between a four-dimensional Randall-Sundrum (RS) braneworld universe compared to a universe without the extra dimension, by considering a black hole solution to the braneworld model that is localized on the brane. When the brane has a negative cosmological constant, then for a certain range of parameters for the black hole, the intersection of the black hole with the brane approximates a Banados-Teitelboim-Zanelli (BTZ) black hole on the brane with corrections that fall off exponentially outside the horizon. We compute the quasinormal modes of the braneworld black hole, and compare them to the known quasinormal modes of the three-dimensional BTZ black hole. We find that there are two distinct regions for the braneworld black hole solutions that are reflected in the dependence of the quasinormal modes on the black hole mass. The imaginary parts of the quasinormal modes display phenomenological similarities to the quasinormal modes of the three-dimensional BTZ black hole, indicating that nonlinear gravitational effects may not be enough to distinguish between a lower-dimensional theory and a theory derived from a higher-dimensional braneworld. Secondly, we consider the evolution of non-extremal black holes in N=4, d=2 supergravity, and investigate how such black holes might evolve over time if perturbed away from extremality. We study this problem in the probe limit by finding tunneling amplitudes for a Dirac field in a single-centered background, which gives the decay rates for the emission of charged probe black holes from the central black hole. We find that there is no minimum to the potential for the probe particles at a finite distance from the central black hole, so any probes that are emitted escape to infinity. If

  18. Nigella sativa (black seed) effects on plasma lipid concentrations in humans: A systematic review and meta-analysis of randomized placebo-controlled trials.

    PubMed

    Sahebkar, Amirhossein; Beccuti, Guglielmo; Simental-Mendía, Luis E; Nobili, Valerio; Bo, Simona

    2016-04-01

    The effects of Nigella sativa (NS) on plasma lipid concentrations are controversial. A systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted to obtain a conclusive result in humans. PubMed-Medline, SCOPUS, Web of Science, and Google Scholar databases were searched (up to August 2015) to identify RCTs investigating the impact of NS on total cholesterol, LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C), and triglycerides concentrations. A random-effects model and the generic inverse variance weighting method were used for quantitative data synthesis. Meta-regression, sensitivity analysis, and publication bias assessments were performed using standard methods. A total of 17 RCTs examining the effects of NS on plasma lipid concentrations were included. Meta-analysis suggested a significant association between NS supplementation and a reduction in total cholesterol (weighed-mean-difference [WMD]: -15.65mg/dL, 95% CI: -24.67, -6.63, p=0.001), LDL-C (WMD: -14.10mg/dL, 95% CI: -19.32, -8.88, p<0.001), and triglyceride levels (WMD: -20.64mg/dL, 95% CI: -30.29, -11.00, p<0.001). No significant effect on HDL-C concentrations (WMD: 0.28mg/dL, 95% CI: -1.96, 2.53, p=0.804) was found. A greater effect of NS seed oil versus seed powder was observed on serum total cholesterol and LDL-C levels, and an increase in HDL-C levels was found only after NS seed powder supplementation. NS has a significant impact on plasma lipid concentrations, leading to lower total cholesterol, LDL-C, and TG levels while increased HDL-C is associated with NS powder only. Further RCTs are needed to explore the NS benefits on cardiovascular outcomes. PMID:26875640

  19. Seed proteomics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Seeds comprise a protective covering, a small embryonic plant, and a nutrient-storage organ. Seeds are protein-rich, and have been the subject of many mass spectrometry-based analyses. Seed storage proteins (SSP), which are transient depots for reduced nitrogen, have been studied for decades by cel...

  20. Spring black stem

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Spring black stem is the most destructive alfalfa diseases in temperate regions of the U.S., Canada, Australia, and countries of Europe, Asia, and South America. The disease causes serious yield losses by reducing canopy dry matter and also decreases seed weight and crown and root mass. Forage qua...

  1. Salinity affects production and salt tolerance of dimorphic seeds of Suaeda salsa.

    PubMed

    Wang, Fengxia; Xu, Yan-Ge; Wang, Shuai; Shi, Weiwei; Liu, Ranran; Feng, Gu; Song, Jie

    2015-10-01

    The effect of salinity on brown seeds/black seeds ratio, seed weight, endogenous hormone concentrations, and germination of brown and black seeds in the euhalophyte Suaeda salsa was investigated. The brown seeds/black seeds ratio, seed weight of brown and black seeds and the content of protein increased at a concentration of 500 mM NaCl compared to low salt conditions (1 mM NaCl). The germination percentage and germination index of brown seeds from plants cultured in 500 mM NaCl were higher than those cultured in 1 mM NaCl, but it was not true for black seeds. The concentrations of IAA (indole-3-acetic acid), ZR (free zeatin riboside) and ABA (abscisic acid) in brown seeds were much greater than those in black seeds, but there were no differences in the level of GAs (gibberellic acid including GA1 and GA3) regardless of the degree of salinity. Salinity during plant culture increased the concentration of GAs, but salinity had no effect on the concentrations of the other three endogenous hormones in brown seeds. Salinity had no effect on the concentration of IAA but increased the concentrations of the other three endogenous hormones in black seeds. Accumulation of endogenous hormones at different concentrations of NaCl during plant growth may be related to seed development and to salt tolerance of brown and black S. salsa seeds. These characteristics may help the species to ensure seedling establishment and population succession in variable saline environments. PMID:26184090

  2. Effects of germination time on seed morph ratio in a seed-dimorphic species and possible ecological significance

    PubMed Central

    Yang, Fan; Baskin, Jerry M.; Baskin, Carol C.; Yang, Xuejun; Cao, Dechang; Huang, Zhenying

    2015-01-01

    Background and Aims Diaspores of heteromorphic species may germinate at different times due to distinct dormancy-breaking and germination requirements, and this difference can influence life history traits. The primary aim of this study was to determine the effect of germination time of the two seed morphs of Suaeda corniculata subsp. mongolica on life history traits of the offspring. Methods Germinated brown and black seeds were sown on the 20th of each month from April to September in a simulated but near-natural habitat of the species. Phenological and vegetative traits of the maternal plants, and number, size and germination percentage of the offspring were determined. Key Results Germinated seeds sown late in the year produced smaller plants that had a higher proportion of non-dormant brown than dormant black seeds, and these brown seeds were larger than those produced by germinated seeds sown early in the year. The length of the seedling stage for brown seeds was shorter than that for black seeds, and the root/shoot ratio and reproductive allocation of plants from brown seeds were more variable than they were for plants from black seeds. Late-germinating brown seeds produced larger plants than late-germinating black seeds. Conclusions Altering the proportion of the two seed types in response to germination timing can help alleviate the adverse effects of delayed germination. The flexible strategy of a species, such as S. corniculata, that produces different proportions of dimorphic seeds in response to variation in germination timing may favour the maintenance and regeneration of the population in its unpredictable environment. PMID:25395107

  3. Probe assembly

    SciTech Connect

    Avera, C.J.

    1981-01-06

    A hand-held probe assembly, suitable for monitoring a radioactive fibrinogen tracer, is disclosed comprising a substantially cylindrically shaped probe handle having an open end. The probe handle is adapted to be interconnected with electrical circuitry for monitoring radioactivity that is sensed or detected by the probe assembly. Mounted within the probe handle is a probe body assembly that includes a cylindrically shaped probe body inserted through the open end of the probe handle. The probe body includes a photomultiplier tube that is electrically connected with a male connector positioned at the rearward end of the probe body. Mounted at the opposite end of the probe body is a probe head which supports an optical coupler therewithin. The probe head is interconnected with a probe cap which supports a detecting crystal. The probe body assembly, which consists of the probe body, the probe head, and the probe cap is supported within the probe handle by means of a pair of compressible o-rings which permit the probe assembly to be freely rotatable, preferably through 360*, within the probe handle and removable therefrom without requiring any disassembly.

  4. Anthocyanin Extracted from Black Soybean Seed Coats Prevents Autoimmune Arthritis by Suppressing the Development of Th17 Cells and Synthesis of Proinflammatory Cytokines by Such Cells, via Inhibition of NF-κB

    PubMed Central

    Baek, Seung-Ye; Woo, Jung-Won; Park, Jin-Sil; Cho, Mi-La; Lee, Jennifer; Kwok, Seung-Ki; Kim, Sae Woong; Park, Sung-Hwan

    2015-01-01

    Introduction Oxidative stress plays a role in the pathogenesis of rheumatoid arthritis (RA). Anthocyanin is a plant antioxidant. We investigated the therapeutic effects of anthocyanin extracted from black soybean seed coats (AEBS) in a murine model of collagen-induced arthritis (CIA) and human peripheral blood mononuclear cells (PBMCs) and explored possible mechanisms by which AEBS might exert anti-arthritic effects. Material and Methods CIA was induced in DBA/1J mice. Cytokine levels were measured via enzyme-linked immunosorbent assays. Joints were assessed in terms of arthritis incidence, clinical arthritis scores, and histological features. The extent of oxidative stress in affected joints was determined by measuring the levels of nitrotyrosine and inducible nitric oxide synthase. NF-κB activity was assayed by measuring the ratio of phosphorylated IκB to total IκB via Western blotting. Th17 cells were stained with antibodies against CD4, IL-17, and STAT3. Osteoclast formation was assessed via TRAP staining and measurement of osteoclast-specific mRNA levels. Results In the CIA model, AEBS decreased the incidence of arthritis, histological inflammation, cartilage scores, and oxidative stress. AEBS reduced the levels of proinflammatory cytokines in affected joints of CIA mice and suppressed NF-κB signaling. AEBS decreased Th17 cell numbers in spleen of CIA mice. Additionally, AEBS repressed differentiation of Th17 cells and expression of Th17-associated genes in vitro, in both splenocytes of naïve DBA/1J mice and human PBMCs. In vitro, the numbers of both human and mouse tartrate-resistant acid phosphatase+ (TRAP) multinucleated cells fell, in a dose-dependent manner, upon addition of AEBS. Conclusions The anti-arthritic effects of AEBS were associated with decreases in Th17 cell numbers, and the levels of proinflammatory cytokines synthesized by such cells, mediated via suppression of NF-κB signaling. Additionally, AEBS suppressed osteoclastogenesis and

  5. Samara Probe For Remote Imaging

    NASA Technical Reports Server (NTRS)

    Burke, James D.

    1989-01-01

    Imaging probe descends through atmosphere of planet, obtaining images of ground surface as it travels. Released from aircraft over Earth or from spacecraft over another planet. Body and single wing shaped like samara - winged seed like those of maple trees. Rotates as descends, providing panoramic view of terrain below. Radio image obtained by video camera to aircraft or spacecraft overhead.

  6. Merging Black Holes

    NASA Technical Reports Server (NTRS)

    Centrella, Joan; Baker, John G.; Kelly, Bernard J.; vanMeter, James R.

    2010-01-01

    Black-hole mergers take place in regions of very strong and dynamical gravitational fields, and are among the strongest sources of gravitational radiation. Probing these mergers requires solving the full set of Einstein's equations of general relativity numerically. For more than 40 years, progress towards this goal has been very slow, as numerical relativists encountered a host of difficult problems. Recently, several breakthroughs have led to dramatic progress, enabling stable and accurate calculations of black-hole mergers. This article presents an overview of this field, including impacts on astrophysics and applications in gravitational wave data analysis.

  7. THE ROLE OF THE EPICUTICULAR WAX LAYER IN WATER MOVEMENT ACROSS THE BEAN SEED COAT

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Water uptake influences a seed's hydration, texture, palatability, and cooking time. Water uptake also influences the leaching of pigments that occurs in color seeded and especially black dry beans (Phaseolus vulgaris L.). Black bean leaching is a major problem in commerce because consumers expect...

  8. Black holes

    PubMed Central

    Brügmann, B.; Ghez, A. M.; Greiner, J.

    2001-01-01

    Recent progress in black hole research is illustrated by three examples. We discuss the observational challenges that were met to show that a supermassive black hole exists at the center of our galaxy. Stellar-size black holes have been studied in x-ray binaries and microquasars. Finally, numerical simulations have become possible for the merger of black hole binaries. PMID:11553801

  9. Black Consciousness

    ERIC Educational Resources Information Center

    Hraba, Joseph; Siegman, Jack

    1974-01-01

    Black militancy is treated as an instance of class consciousness with criteria and scales developed to measure black consciousness and "self-placement" into black consciousness. These dimensions are then investigated with respect to the social and symbolic participation in the ideology of the black movement on the part of a sample of black…

  10. Project SEED.

    ERIC Educational Resources Information Center

    Chemical and Engineering News, 1986

    1986-01-01

    Reports on Project SEED (Summer Educational Experience for the Disadvantaged) a project in which high school students from low-income families work in summer jobs in a variety of academic, industrial, and government research labs. The program introduces the students to career possibilities in chemistry and to the advantages of higher education.…

  11. Viability prediction of Ricinus cummunis L. seeds using multispectral imaging.

    PubMed

    Olesen, Merete Halkjær; Nikneshan, Pejman; Shrestha, Santosh; Tadayyon, Ali; Deleuran, Lise Christina; Boelt, Birte; Gislum, René

    2015-01-01

    The purpose of this study was to highlight the use of multispectral imaging in seed quality testing of castor seeds. Visually, 120 seeds were divided into three classes: yellow, grey and black seeds. Thereafter, images at 19 different wavelengths ranging from 375-970 nm were captured of all the seeds. Mean intensity for each single seed was extracted from the images, and a significant difference between the three colour classes was observed, with the best separation in the near-infrared wavelengths. A specified feature (RegionMSI mean) based on normalized canonical discriminant analysis, were employed and viable seeds were distinguished from dead seeds with 92% accuracy. The same model was tested on a validation set of seeds. These seeds were divided into two groups depending on germination ability, 241 were predicted as viable and expected to germinate and 59 were predicted as dead or non-germinated seeds. This validation of the model resulted in 96% correct classification of the seeds. The results illustrate how multispectral imaging technology can be employed for prediction of viable castor seeds, based on seed coat colour. PMID:25690554

  12. Viability Prediction of Ricinus cummunis L. Seeds Using Multispectral Imaging

    PubMed Central

    Olesen, Merete Halkjær; Nikneshan, Pejman; Shrestha, Santosh; Tadayyon, Ali; Deleuran, Lise Christina; Boelt, Birte; Gislum, René

    2015-01-01

    The purpose of this study was to highlight the use of multispectral imaging in seed quality testing of castor seeds. Visually, 120 seeds were divided into three classes: yellow, grey and black seeds. Thereafter, images at 19 different wavelengths ranging from 375–970 nm were captured of all the seeds. Mean intensity for each single seed was extracted from the images, and a significant difference between the three colour classes was observed, with the best separation in the near-infrared wavelengths. A specified feature (RegionMSI mean) based on normalized canonical discriminant analysis, were employed and viable seeds were distinguished from dead seeds with 92% accuracy. The same model was tested on a validation set of seeds. These seeds were divided into two groups depending on germination ability, 241 were predicted as viable and expected to germinate and 59 were predicted as dead or non-germinated seeds. This validation of the model resulted in 96% correct classification of the seeds. The results illustrate how multispectral imaging technology can be employed for prediction of viable castor seeds, based on seed coat colour. PMID:25690554

  13. Black holes and the multiverse

    NASA Astrophysics Data System (ADS)

    Garriga, Jaume; Vilenkin, Alexander; Zhang, Jun

    2016-02-01

    Vacuum bubbles may nucleate and expand during the inflationary epoch in the early universe. After inflation ends, the bubbles quickly dissipate their kinetic energy; they come to rest with respect to the Hubble flow and eventually form black holes. The fate of the bubble itself depends on the resulting black hole mass. If the mass is smaller than a certain critical value, the bubble collapses to a singularity. Otherwise, the bubble interior inflates, forming a baby universe, which is connected to the exterior FRW region by a wormhole. A similar black hole formation mechanism operates for spherical domain walls nucleating during inflation. As an illustrative example, we studied the black hole mass spectrum in the domain wall scenario, assuming that domain walls interact with matter only gravitationally. Our results indicate that, depending on the model parameters, black holes produced in this scenario can have significant astrophysical effects and can even serve as dark matter or as seeds for supermassive black holes. The mechanism of black hole formation described in this paper is very generic and has important implications for the global structure of the universe. Baby universes inside super-critical black holes inflate eternally and nucleate bubbles of all vacua allowed by the underlying particle physics. The resulting multiverse has a very non-trivial spacetime structure, with a multitude of eternally inflating regions connected by wormholes. If a black hole population with the predicted mass spectrum is discovered, it could be regarded as evidence for inflation and for the existence of a multiverse.

  14. Extreme black hole holography

    NASA Astrophysics Data System (ADS)

    Hartman, Thomas Edward

    The connection between black holes in four dimensions and conformal field theories (CFTs) in two dimensions is explored, focusing on zero temperature (extreme) black holes and their low-temperature cousins. It is shown that extreme black holes in a theory of quantum gravity are holographically dual to field theories living in two dimensions without gravity, and that the field theory reproduces a variety of black hole phenomena in detail. The extreme black hole/CFT correspondence is derived from a symmetry analysis near the horizon of a Kerr black hole with mass M and maximal angular momentum J=M 2. The asymptotic symmetry generators form one copy of the Virasoro algebra with central charge c=12J, which implies that the near-horizon quantum states are identical to those of a two-dimensional CFT. We discuss extensions of this result to near-extreme black holes and cosmological horizons. Astrophysical black holes are never exactly extremal, but the black hole GRS1915+105 observed through X-ray and radio telescopy is likely within 1% of the extremal spin, suggesting that this extraordinary and well studied object is approximately dual to a two-dimensional CFT with c˜1079. As evidence for the correspondence, microstate counting in the CFT is used to derive the Bekenstein-Hawking area law for the Kerr entropy, S=Horizon area/4. Furthermore, the correlators in the dual CFT are shown to reproduce the scattering amplitudes of a charged scalar or spin-½ field by a near-extreme Kerr-Newman black hole, and a neutral spin-1 or spin-2 field by a near-extreme Kerr black hole. Scattering amplitudes probe the vacuum of fields living on the black hole background. For scalars, bound superradiant modes lead to an instability, while for fermions, it is shown that the bound superradiant modes condense and form a Fermi sea which extends well outside the ergosphere. Assuming no further instabilities, the low energy effective theory near the black hole is described by ripples in the

  15. Tomograms of spinning black holes

    NASA Astrophysics Data System (ADS)

    Krishnan, Chethan

    2009-12-01

    The classical internal structure of spinning black holes is vastly different from that of static black holes. We consider spinning Banados-Teitelboim-Zanelli black holes, and probe their interior from the gauge theory. Utilizing the simplicity of the geometry and reverse engineering from the geodesics, we propose a thermal correlator construction which can be interpreted as arising from two entangled conformal field theories. By analytic continuation of these correlators, we can probe the Cauchy horizon. Correlators that capture the Cauchy horizon in our work have a structure closely related to those that capture the singularity in a nonrotating Banados-Teitelboim-Zanelli. As expected, the regions beyond the Cauchy horizon are not probed in this picture, protecting cosmic censorship.

  16. Black Holes

    NASA Astrophysics Data System (ADS)

    Livio, Mario; Koekemoer, Anton M.

    2011-02-01

    Participants; Preface Mario Livio and Anton Koekemoer; 1. Black holes, entropy, and information G. T. Horowitz; 2. Gravitational waves from black-hole mergers J. G. Baker, W. D. Boggs, J. M. Centrella, B. J. Kelley, S. T. McWilliams and J. R. van Meter; 3. Out-of-this-world physics: black holes at future colliders G. Landsberg; 4. Black holes in globular clusters S. L. W. McMillan; 5. Evolution of massive black holes M. Volonteri; 6. Supermassive black holes in deep multiwavelength surveys C. M. Urry and E. Treister; 7. Black-hole masses from reverberation mapping B. M. Peterson and M. C. Bentz; 8. Black-hole masses from gas dynamics F. D. Macchetto; 9. Evolution of supermassive black holes A. Müller and G. Hasinger; 10. Black-hole masses of distant quasars M. Vestergaard; 11. The accretion history of supermassive black holes K. Brand and the NDWFS Boötes Survey Teams; 12. Strong field gravity and spin of black holes from broad iron lines A. C. Fabian; 13. Birth of massive black-hole binaries M. Colpi, M. Dotti, L. Mayer and S. Kazantzidis; 14. Dynamics around supermassive black holes A. Gualandris and D. Merritt; 15. Black-hole formation and growth: simulations in general relativity S. L. Shapiro; 16. Estimating the spins of stellar-mass black holes J. E. McClintock, R. Narayan and R. Shafee; 17. Stellar relaxation processes near the Galactic massive black hole T. Alexander; 18. Tidal disruptions of stars by supermassive black holes S. Gezari; 19. Where to look for radiatively inefficient accretion flows in low-luminosity AGN M. Chiaberge; 20. Making black holes visible: accretion, radiation, and jets J. H. Krolik.

  17. Spectrophotometric probe

    DOEpatents

    Prather, W.S.; O'Rourke, P.E.

    1994-08-02

    A support structure is described bearing at least one probe for making spectrophotometric measurements of a fluid using a source of light and a spectrophotometer. The probe includes a housing with two optical fibers and a planoconvex lens. A sleeve bearing a mirror surrounds the housing. The lens is separated from the mirror by a fixed distance, defining an interior space for receiving a volume of the fluid sample. A plurality of throughholes extending through the sleeve communicate between the sample volume and the exterior of the probe, all but one hole bearing a screen. A protective jacket surrounds the probe. A hollow conduit bearing a tube is formed in the wall of the probe for venting any air in the interior space when fluid enters. The probe is held at an acute angle so the optic fibers carrying the light to and from the probe are not bent severely on emergence from the probe. 3 figs.

  18. Spectrophotometric probe

    DOEpatents

    Prather, William S.; O'Rourke, Patrick E.

    1994-01-01

    A support structure bearing at least one probe for making spectrophotometric measurements of a fluid using a source of light and a spectrophotometer. The probe includes a housing with two optical fibers and a planoconvex lens. A sleeve bearing a mirror surrounds the housing. The lens is separated from the mirror by a fixed distance, defining an interior space for receiving a volume of the fluid sample. A plurality of throughholes extending through the sleeve communicate between the sample volume and the exterior of the probe, all but one hole bearing a screen. A protective jacket surrounds the probe. A hollow conduit bearing a tube is formed in the wall of the probe for venting any air in the interior space when fluid enters. The probe is held at an acute angle so the optic fibers carrying the light to and from the probe are not bent severely on emergence from the probe.

  19. Seed bank dynamics of invasive swallowworts

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pale swallowwort (SW) (Vincetoxicum rossicum) and black swallowwort (V. nigrum; Apocynaceae, subfamily Asclepiadoideae) are European viny milkweeds that have become invasive in many habitats in the northeastern U.S.A. and southeastern Canada. A multi-year seed bank study was initiated in fall 2011 t...

  20. Swallowwort dispersal: seed tracking under field conditions

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Vincetoxicum rossicum (pale swallowwort) and V. nigrum (black swallowwort) are invasive, perennial vines found in natural areas of the northeastern United States and southeastern Canada. Seeds of both species are primarily wind-dispersed in the form of achenes with an attached coma. For this study,...

  1. Interaction of microwaves and germinating seeds

    SciTech Connect

    Shafer, F.L.

    1987-01-01

    The preliminary investigation measured the internal metabolic process by ATP production. Leakage of ions and organic material from germinating seeds indicated that membranes are a target of microwaves and heat. Electron photo-micrographs showed an increase in damage to membranes as heat and microwave treatments were increased. The second phase of this investigation was concerned with determining some of the biological activity at the initiation of germination of wheat seed, Triticum aestivum L., using a resonating microwave cavity oscillating at 9.3 GHz as a probe. Direct current conductivity measurements were also made on the seeds as a means of confirming the observations made with the microwave cavity.

  2. Black ring deconstruction

    SciTech Connect

    Gimon, Eric; Gimon, Eric G.; Levi, Thomas S.

    2007-06-22

    We present a sample microstate for a black ring in four and five dimensional language. The microstate consists of a black string microstate with an additional D6-brane. We show that with an appropriate choice of parameters the piece involving the black string microstate falls down a long AdS throat, whose M-theory lift is AdS_3 x S2. We wrap a spinning dipole M2-brane on the S2 in the probe approximation. In IIA, this corresponds to a dielectric D2-brane carrying only D0-charge. We conjecture this is the firstapproximation to a cloud of D0-branes blowing up due to their non-abelian degrees of freedom and the Myers effect.

  3. Black Art.

    ERIC Educational Resources Information Center

    Baraka, Amiri

    1987-01-01

    Discusses black art as not only an expression of black life but as revolutionary art. It must be collective, functional, and committing. It must also be anti-racist, anti-capitalist, and anti-imperialist. (LHW)

  4. Black tea

    MedlinePlus

    ... that the caffeine in black tea might slow blood clotting, though this hasn’t been shown in people. ... Talk with your health provider.Medications that slow blood clotting (Anticoagulant / Antiplatelet drugs)Black tea contains caffeine. Caffeine ...

  5. Black Ageism

    ERIC Educational Resources Information Center

    Golden, Herbert M.

    1976-01-01

    Notes that attempts to apply research findings based on undifferentiated comparisons between black and white elderly toward the solution of problems faced by black elderly are doomed to ineffectiveness. (Author/AM)

  6. Black psyllium

    MedlinePlus

    Black psyllium is a weed that grows aggressively throughout the world. The plant was spread with the ... to make medicine. Be careful not to confuse black psyllium with other forms of psyllium including blond ...

  7. Black tea

    MedlinePlus

    Black tea is a product made from the Camellia sinesis plant. The aged leaves and stems are ... of the same plant, has some different properties. Black tea is used for improving mental alertness as ...

  8. Black Cohosh

    MedlinePlus

    ... gov Key References Black cohosh. Natural Medicines Comprehensive Database Web site. Accessed at www.naturaldatabase.com on April ... Black cohosh ( Cimicifuga racemosa [L.] Nutt. ). Natural Standard Database Web site. Accessed at www.naturalstandard.com on April ...

  9. Seed Treatment. Bulletin 760.

    ERIC Educational Resources Information Center

    Lowery, Harvey C.

    This manual gives a definition of seed treatment, the types of seeds normally treated, diseases and insects commonly associated with seeds, fungicides and insecticides used, types of equipment used for seed treatment, and information on labeling and coloring of treated seed, pesticide carriers, binders, stickers, and safety precautions. (BB)

  10. Black Students.

    ERIC Educational Resources Information Center

    Edwards, Harry

    The black student revolt did not start with the highly publicized activities of the black students at San Francisco State College. The roots of the revolt lie deeply imbedded within the history and structure of the overall black liberation struggle in America. The beginnings of this revolt can be found in the students of Southern Negro colleges in…

  11. Talking Black.

    ERIC Educational Resources Information Center

    Abrahams, Roger D.

    This book contains essays which focus on the systems of communication that operate within and between various social segments of Afro-American communities in the United States. The essays are presented under the following headings: (1) "Getting Into It: Black Talk, Black Life and the Academic," (2) "'Talking My Talk': Black Talk Varieties and…

  12. Black Appalachians.

    ERIC Educational Resources Information Center

    Waage, Fred, Ed.; Cabbell, Ed, Ed.

    1986-01-01

    This issue of "Now and Then" focuses on black Appalachians, their culture, and their history. It contains local histories, articles, and poems and short stories by Appalachian blacks. Articles include: "A Mountain Artist's Landscape," a profile of artist Rita Bradley by Pat Arnow; "A Part and Apart," a profile of black historian Ed Cabbell by Pat…

  13. Black Psychology.

    ERIC Educational Resources Information Center

    Jones, Reginald L., Ed.

    The contents of the present volume, designed to bring together in a single place writings by the new black psychologists and other black social and behavioral scientists, are organized in seven parts, as follows: Part I, "Black Psychology: Perspectives," includes articles by Cedric Clark, Wade W. Nobles, Doris P. Mosby, Joseph White, and William…

  14. Black Holes

    NASA Astrophysics Data System (ADS)

    Luminet, Jean-Pierre

    1992-09-01

    Foreword to the French edition; Foreword to the English edition; Acknowledgements; Part I. Gravitation and Light: 1. First fruits; 2. Relativity; 3. Curved space-time; Part II. Exquisite Corpses: 4. Chronicle of the twilight years; 5. Ashes and diamonds; 6. Supernovae; 7. Pulsars; 8. Gravitation triumphant; Part III. Light Assassinated: 9. The far horizon; 10. Illuminations; 11. A descent into the maelstrom; 12. Map games; 13. The black hole machine; 14. The quantum black hole; Part IV. Light Regained: 15. Primordial black holes; 16. The zoo of X-ray stars; 17. Giant black holes; 18. Gravitational light; 19. The black hole Universe; Appendices; Bibliography; Name index; Subject index.

  15. What Are Chia Seeds?

    MedlinePlus

    ... Men For Women For Seniors What Are Chia Seeds? Published February 05, 2014 Print Email When you ... number of research participants. How to Eat Chia Seeds Chia seeds can be eaten raw or prepared ...

  16. Pigmented Soybean (Glycine max) Seed Coats Accumulate Proanthocyanidins during Development.

    PubMed Central

    Todd, J. J.; Vodkin, L. O.

    1993-01-01

    The dominant I gene inhibits accumulation of anthocyanin pigments in the epidermal layer of soybean (Glycine max) seed coats. Seed-coat color is also influenced by the R locus and by the pubescence color alleles (T, tawny; t, gray). Protein and RNA from cultivars with black (i,R,T) and brown (i,r,T) seed coats are difficult to extract. To determine the nature of the interfering plant products, we examined seed-coat extracts from Clark isogenic lines for flavonoids, anthocyanins, and possible proanthocyanidins by thin-layer chromatography. We show that yellow seed-coat varieties (I) do not accumulate anthocyanins (anthocyanidin glycosides) or proanthocyanidins (polymeric anthocyanidins). Mature, black (i,R,T) and imperfect-black (i,R,t) seed coats contained anthocyanins, whereas mature, brown (i,r,T) and buff (i,r,t) seed coats did not contain anthocyanins. In contrast, all colored (i) genotypes tested positive for the presence of proanthocyanidins by butanol/ HCl and 0.5% vanillin assays. Immature, black (i,R,T) and brown (i,r,T) seed coats contained significant amounts of procyanidin, a 3[prime],4[prime]-hydroxylated proanthocyanidin. Immature, black (i,R,T) or brown (i,r,T) seed-coat extracts also tested positive for the ability to precipitate proteins in a radial diffusion assay and to bind RNA in vitro. Imperfect-black (i,R,t) or buff (i,r,t) seed coats contained lesser amounts of propelargonidin, a 4[prime]-hydroxylated proanthocyanidin. Seed-coat extracts from these genotypes did not have the ability to precipitate protein or bind to RNA. In summary, the dominant I gene controls inhibition of not only anthocyanins but also proanthocyanidins in soybean seed coats. In homozygous recessive i genotypes, the T-t gene pair determines the types of proanthocyanidins present, which is consistent with the hypothesis that the T locus encodes a microsomal 3[prime]-flavonoid hydroxylase. PMID:12231856

  17. Phantom black holes and sigma models

    SciTech Connect

    Azreg-Aienou, Mustapha; Clement, Gerard; Fabris, Julio C.; Rodrigues, Manuel E.

    2011-06-15

    We construct static multicenter solutions of phantom Einstein-Maxwell-dilaton theory from null geodesics of the target space, leading to regular black holes without spatial symmetry for certain discrete values of the dilaton coupling constant. We also discuss the three-dimensional gravitating sigma models obtained by reduction of phantom Einstein-Maxwell, phantom Kaluza-Klein and phantom Einstein-Maxwell-dilaton-axion theories. In each case, we generate by group transformations phantom charged black hole solutions from a neutral seed.

  18. Seed health and vigor

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The health of lentil and chickpea seed greatly impacts the quality of the crop stand and yield. Healthy seed has a high germination rate, is whole (free of cracks or other damage), is free from foreign matter including weed seed and has limited seedborne pathogens. The health of the seed often dep...

  19. Multibeam seeded brillouin sidescatter in inertial confinement fusion experiments.

    PubMed

    Turnbull, D; Michel, P; Ralph, J E; Divol, L; Ross, J S; Berzak Hopkins, L F; Kritcher, A L; Hinkel, D E; Moody, J D

    2015-03-27

    We present the first observations of multibeam weakly seeded Brillouin sidescatter in indirect-drive inertial confinement fusion (ICF) experiments. Two seeding mechanisms have been identified and quantified: specular reflections ("glint") from opposite hemisphere beams, and Brillouin backscatter from neighboring beams with a different angle of incidence. Seeded sidescatter can dominate the overall coupling losses, so understanding this process is crucial for proper accounting of energy deposition and drive symmetry. Glint-seeded scattered light could also be used to probe hydrodynamic conditions inside ICF targets. PMID:25860748

  20. Black hole accretion disc impacts

    NASA Astrophysics Data System (ADS)

    Pihajoki, P.

    2016-04-01

    We present an analytic model for computing the luminosity and spectral evolution of flares caused by a supermassive black hole impacting the accretion disc of another supermassive black hole. Our model includes photon diffusion, emission from optically thin regions and relativistic corrections to the observed spectrum and time-scales. We test the observability of the impact scenario with a simulated population of quasars hosting supermassive black hole binaries. The results indicate that for a moderate binary mass ratio of 0.3, and impact distances of 100 primary Schwarzschild radii, the accretion disc impacts can be expected to equal or exceed the host quasar in brightness at observed wavelength λ = 510 nm up to z = 0.6. We conclude that accretion disc impacts may function as an independent probe for supermassive black hole binaries. We release the code used for computing the model light curves to the community.

  1. Optical probe

    DOEpatents

    Hencken, Kenneth; Flower, William L.

    1999-01-01

    A compact optical probe is disclosed particularly useful for analysis of emissions in industrial environments. The instant invention provides a geometry for optically-based measurements that allows all optical components (source, detector, rely optics, etc.) to be located in proximity to one another. The geometry of the probe disclosed herein provides a means for making optical measurements in environments where it is difficult and/or expensive to gain access to the vicinity of a flow stream to be measured. Significantly, the lens geometry of the optical probe allows the analysis location within a flow stream being monitored to be moved while maintaining optical alignment of all components even when the optical probe is focused on a plurality of different analysis points within the flow stream.

  2. Black Men.

    ERIC Educational Resources Information Center

    Gary, Lawrence E., Ed.

    The essays in this book examine some of the major issues affecting the behavior and status of black men in the United States. The volume is divided into four sections. Part one compares black and white men on such indicators as sex ratio, age distribution, marital and family status, educational attainment, employment, income, social and political…

  3. Fast radioactive seed localization in intraoperative cone beam CT for low-dose-rate prostate brachytherapy

    NASA Astrophysics Data System (ADS)

    Hu, Yu-chi; Xiong, Jian-ping; Cohan, Gilad; Zaider, Marco; Mageras, Gig; Zelefsky, Michael

    2013-03-01

    A fast knowledge-based radioactive seed localization method for brachytherapy was developed to automatically localize radioactive seeds in an intraoperative volumetric cone beam CT (CBCT) so that corrections, if needed, can be made during prostate implant surgery. A transrectal ultrasound (TRUS) scan is acquired for intraoperative treatment planning. Planned seed positions are transferred to intraoperative CBCT following TRUS-to-CBCT registration using a reference CBCT scan of the TRUS probe as a template, in which the probe and its external fiducial markers are pre-segmented and their positions in TRUS are known. The transferred planned seeds and probe serve as an atlas to reduce the search space in CBCT. Candidate seed voxels are identified based on image intensity. Regions are grown from candidate voxels and overlay regions are merged. Region volume and intensity variance is checked against known seed volume and intensity profile. Regions meeting the above criteria are flagged as detected seeds; otherwise they are flagged as likely seeds and sorted by a score that is based on volume, intensity profile and distance to the closest planned seed. A graphical interface allows users to review and accept or reject likely seeds. Likely seeds with approximately twice the seed volume are automatically split. Five clinical cases are tested. Without any manual correction in seed detection, the method performed the localization in 5 seconds (excluding registration time) for a CBCT scan with 512×512×192 voxels. The average precision rate per case is 99% and the recall rate is 96% for a total of 416 seeds. All false negative seeds are found with 15 in likely seeds and 1 included in a detected seed. With the new method, updating of calculations of dose distribution during the procedure is possible and thus facilitating evaluation and improvement of treatment quality.

  4. Bringing Black Holes Together: How Supermassive Black Hole Binaries Form and Plunge Through the Final Parsec

    NASA Astrophysics Data System (ADS)

    Holley-Bockelmann, Kelly

    2016-04-01

    Astronomers now know that supermassive black holes reside in nearly every galaxy.Though these black holes are an observational certainty, nearly every aspect of their evolution -- from their birth, to their fuel source, to their basic dynamics -- is a matter of lively debate. In principle, gas-rich major galaxy mergers can generate the central stockpile of fuel needed for a low mass central black hole seed to grow quickly into a supermassive one. During a galaxy merger, the black holes in each galaxy meet and form a supermassive binary black hole; as the binary orbit shrinks through its final parsec, it becomes the loudest gravitational wave source in the Universe and a powerful agent to sculpt the galactic center. This talk will touch on some current and ongoing work on refining our theories of how supermassive black hole binaries form, evolve within, and alter their galaxy host.

  5. Image-processing algorithms for inspecting characteristics of hybrid rice seed

    NASA Astrophysics Data System (ADS)

    Cheng, Fang; Ying, Yibin

    2004-03-01

    Incompletely closed glumes, germ and disease are three characteristics of hybrid rice seed. Image-processing algorithms developed to detect these seed characteristics were presented in this paper. The rice seed used for this study involved five varieties of Jinyou402, Shanyou10, Zhongyou207, Jiayou and IIyou. The algorithms were implemented with a 5*600 images set, a 4*400 images set and the other 5*600 images set respectively. The image sets included black background images, white background images and both sides images of rice seed. Results show that the algorithm for inspecting seeds with incompletely closed glumes based on Radon Transform achieved an accuracy of 96% for normal seeds, 92% for seeds with fine fissure and 87% for seeds with unclosed glumes, the algorithm for inspecting germinated seeds on panicle based on PCA and ANN achieved n average accuracy of 98% for normal seeds, 88% for germinated seeds on panicle and the algorithm for inspecting diseased seeds based on color features achieved an accuracy of 92% for normal and healthy seeds, 95% for spot diseased seeds and 83% for severe diseased seeds.

  6. Genetic control of the seed coat colour of Middle American and Andean bean seeds.

    PubMed

    Possobom, Micheli Thaise Della Flora; Ribeiro, Nerinéia Dalfollo; Zemolin, Allan Emanoel Mezzomo; Arns, Fernanda Daltrozo

    2015-02-01

    Seed coat colour of bean seeds is decisive for acceptance of a cultivar. The objectives of this research were to determine whether there is maternal effect for "L", a* and b* colour parameters in Middle American and Andean bean seeds; to obtain estimates of heritability and gain with selection for "L", a* and b* values; and select recombinants with the seed coat colour required by the market demand. Thus, controlled crossings were carried out between the Middle American lines CNFP 10104 and CHC 01-175, and between the Andean lines Cal 96 and Hooter, for obtaining F1, F1 reciprocal, F2 and F2 reciprocal generations for each hybrid combination. Parents and generations were evaluated in two field experiments (2012 normal rainy and 2013 dry seasons) in the state of Rio Grande do Sul, Brazil. Seed coat colour was quantified with a portable colorimeter. Genetic variability for "L" (luminosity), chromaticity a* (green to red shade), and chromaticity b* (blue to yellow shade) values was observed in seeds with F2 seed coat of Middle American and Andean beans. "L", a* and b* values in bean seeds presented maternal effects. High broad-sense heritability are observed for luminosity (h(2)b: 76.66-95.07%), chromaticity a* (h(2)b: 73.08-89.31%), and chromaticity b* (h(2)b: 88.63-92.50%) values in bean seeds. From the crossings, it was possible to select bean seeds in early generation for the black group, and for carioca and cranberry types (dark or clear background) which present the colour required by the market demand. PMID:25523544

  7. Hidden conformal symmetry of extremal black holes

    SciTech Connect

    Chen Bin; Long Jiang; Zhang Jiaju

    2010-11-15

    We study the hidden conformal symmetry of extremal black holes. We introduce a new set of conformal coordinates to write the SL(2,R) generators. We find that the Laplacian of the scalar field in many extremal black holes, including Kerr(-Newman), Reissner-Nordstrom, warped AdS{sub 3}, and null warped black holes, could be written in terms of the SL(2,R) quadratic Casimir. This suggests that there exist dual conformal field theory (CFT) descriptions of these black holes. From the conformal coordinates, the temperatures of the dual CFTs could be read directly. For the extremal black hole, the Hawking temperature is vanishing. Correspondingly, only the left (right) temperature of the dual CFT is nonvanishing, and the excitations of the other sector are suppressed. In the probe limit, we compute the scattering amplitudes of the scalar off the extremal black holes and find perfect agreement with the CFT prediction.

  8. Patterns of Cross-Continental Variation in Tree Seed Mass in the Canadian Boreal Forest

    PubMed Central

    Liu, Jushan; Bai, Yuguang; Lamb, Eric G.; Simpson, Dale; Liu, Guofang; Wei, Yongsheng; Wang, Deli; McKenney, Daniel W.; Papadopol, Pia

    2013-01-01

    Seed mass is an adaptive trait affecting species distribution, population dynamics and community structure. In widely distributed species, variation in seed mass may reflect both genetic adaptation to local environments and adaptive phenotypic plasticity. Acknowledging the difficulty in separating these two aspects, we examined the causal relationships determining seed mass variation to better understand adaptability and/or plasticity of selected tree species to spatial/climatic variation. A total of 504, 481 and 454 seed collections of black spruce (Picea mariana (Mill.) B.S.P.), white spruce (Picea glauca (Moench) Voss) and jack pine (Pinus banksiana Lamb) across the Canadian Boreal Forest, respectively, were selected. Correlation analyses were used to determine how seed mass vary with latitude, longitude, and altitude. Structural Equation Modeling was used to examine how geographic and climatic variables influence seed mass. Climatic factors explained a large portion of the variation in seed mass (34, 14 and 29%, for black spruce, white spruce and jack pine, respectively), indicating species-specific adaptation to long term climate conditions. Higher annual mean temperature and winter precipitation caused greater seed mass in black spruce, but annual precipitation was the controlling factor for white spruce. The combination of factors such as growing season temperature and evapotranspiration, temperature seasonality and annual precipitation together determined seed mass of jack pine. Overall, sites with higher winter temperatures were correlated with larger seeds. Thus, long-term climatic conditions, at least in part, determined spatial variation in seed mass. Black spruce and Jack pine, species with relatively more specific habitat requirements and less plasticity, had more variation in seed mass explained by climate than did the more plastic species white spruce. As traits such as seed mass are related to seedling growth and survival, they potentially

  9. Conductivity Probe

    NASA Technical Reports Server (NTRS)

    2008-01-01

    The Thermal and Electrical Conductivity Probe (TECP) for NASA's Phoenix Mars Lander took measurements in Martian soil and in the air.

    The needles on the end of the instrument were inserted into the Martian soil, allowing TECP to measure the propagation of both thermal and electrical energy. TECP also measured the humidity in the surrounding air.

    The needles on the probe are 15 millimeters (0.6 inch) long.

    The Phoenix Mission is led by the University of Arizona, Tucson, on behalf of NASA. Project management of the mission is by NASA's Jet Propulsion Laboratory, Pasadena, Calif. Spacecraft development is by Lockheed Martin Space Systems, Denver.

  10. Black Hills

    Atmospheric Science Data Center

    2014-05-15

    ... surfaces with lower absorption appear as green, yellow, orange or red. Black pixels indicate areas where albedo could not be derived, ... notably reduced in extent, and higher albedo areas (yellow, orange and red pixels) have increased. Because incoming sunlight is ...

  11. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds...

  12. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds...

  13. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds...

  14. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds...

  15. 7 CFR 201.18 - Other agricultural seeds (crop seeds).

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Other agricultural seeds (crop seeds). 201.18 Section... SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE (CONTINUED) FEDERAL SEED ACT FEDERAL SEED ACT REGULATIONS Labeling Agricultural Seeds § 201.18 Other agricultural seeds...

  16. Pollution Probe.

    ERIC Educational Resources Information Center

    Chant, Donald A.

    This book is written as a statement of concern about pollution by members of Pollution Probe, a citizens' anti-pollution group in Canada. Its purpose is to create public awareness and pressure for the eventual solution to pollution problems. The need for effective government policies to control the population explosion, conserve natural resources,…

  17. Pasture seed banks

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In our surveys of northeastern pastures, we found the equivalent of more than 8 million seeds per acre in the surface soil (the top four inches) from the seed bank study. These seeds came from 58 species of plants. The annual forbs (all broadleaf plants with the exception of legumes and trees) domin...

  18. Preservation of recalcitrant seeds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Recalcitrant and intermediate seeds are not included in seed banks because of misperceptions that these efforts would be futile. Between 20 and 25% of the Earth’s angiosperm species are estimated to produce recalcitrant or intermediate seeds. These species are more prevalent in the tropics and sub...

  19. Seed Development and Germination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Seed is the fertilized and matured ovule of angiosperms and gymnosperms and represents a crucial stage in the life cycle of plants. Seeds of diverse plant species may display differences in size, shape and color. Despite apparent morphological variations, most mature seeds consist of three major com...

  20. Seed Proteomics"

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Proteomic analysis of seeds encounters some specific problems that do not impinge on analyses of other plant cells, tissues, or organs. There are anatomic considerations. Seeds comprise the seed coat, the storage organ(s), and the embryonic axis. Are these to be studied individually or as a compo...

  1. Going to Seed.

    ERIC Educational Resources Information Center

    Powell, Richard R.

    1984-01-01

    Describes a unit on seeds designed to introduce students to their scientific and nutritional uses. Unit activities are easily done, employ a variety of process skills, and can be used at various grade levels. Suggests field trips to gather seeds, seed sprouting, and making cookies out of various whole grains. (JM)

  2. The formation of the first black holes in the Universe

    NASA Astrophysics Data System (ADS)

    Natarajan, Priyamvada

    2012-07-01

    Black holes are the most enigmatic objects in the Universe, although it appears now that they are ubiquitous and inhabit the centers of most galaxies in the Universe. In fact, the observed local demography of black holes in galactic nuclei suggests that they are supermassive. The first black holes are expected to have formed from the remnants of the first stars at very early times. However, the discovery of supermassive black holes that power quasars as early as 2 Gyrs after the Big Bang requires alternate channels to form more massive seed black holes. We proposed a new mechanism that involves the direct collapse of massive pre-galactic gas disks and thereby makes massive early seeds to alleviate this problem. I will present the latest results from the theoretical investigation of these models including state of the art numerical simulations of the formation process and outline the new tantalizing observational evidence that appears to support such a mechanism.

  3. Dark Candles of the Universe: Black Hole Observations

    NASA Astrophysics Data System (ADS)

    Aykutalp, Aycin

    2016-03-01

    In 1916, when Karl Schwarzschild solved the Einstein field equations of general relativity for a spherically symmetric, non-rotating mass no one anticipated the impact black holes would have on astrophysics. I will review the main formation channels for black hole seeds and their evolution through cosmic time. In this, emphasis will be placed on the observational diagnostics of astrophysical black holes and their role on the assembly of galaxy formation and evolution. I then review how these observations put constrain on the seed black hole formation theories. Finally, I present an outlook for how future observations can shed light on our understanding of black holes. This work is supported by NSF Grant AST-1333360.

  4. The Crisis in Black and Black.

    ERIC Educational Resources Information Center

    Hutchinson, Earl Ofari

    These essays explore why the historic conflict between blacks and whites in the United States has become a crisis that divides many African Americans. The changing racial dynamic is not marked by conflicts. between the black middle class and the poor, black men and women, the black intellectual elite and rappers, black politicians and the urban…

  5. Ice-Borehole Probe

    NASA Technical Reports Server (NTRS)

    Behar, Alberto; Carsey, Frank; Lane, Arthur; Engelhardt, Herman

    2006-01-01

    An instrumentation system has been developed for studying interactions between a glacier or ice sheet and the underlying rock and/or soil. Prior borehole imaging systems have been used in well-drilling and mineral-exploration applications and for studying relatively thin valley glaciers, but have not been used for studying thick ice sheets like those of Antarctica. The system includes a cylindrical imaging probe that is lowered into a hole that has been bored through the ice to the ice/bedrock interface by use of an established hot-water-jet technique. The images acquired by the cameras yield information on the movement of the ice relative to the bedrock and on visible features of the lower structure of the ice sheet, including ice layers formed at different times, bubbles, and mineralogical inclusions. At the time of reporting the information for this article, the system was just deployed in two boreholes on the Amery ice shelf in East Antarctica and after successful 2000 2001 deployments in 4 boreholes at Ice Stream C, West Antarctica, and in 2002 at Black Rapids Glacier, Alaska. The probe is designed to operate at temperatures from 40 to +40 C and to withstand the cold, wet, high-pressure [130-atm (13.20-MPa)] environment at the bottom of a water-filled borehole in ice as deep as 1.6 km. A current version is being outfitted to service 2.4-km-deep boreholes at the Rutford Ice Stream in West Antarctica. The probe (see figure) contains a sidelooking charge-coupled-device (CCD) camera that generates both a real-time analog video signal and a sequence of still-image data, and contains a digital videotape recorder. The probe also contains a downward-looking CCD analog video camera, plus halogen lamps to illuminate the fields of view of both cameras. The analog video outputs of the cameras are converted to optical signals that are transmitted to a surface station via optical fibers in a cable. Electric power is supplied to the probe through wires in the cable at a

  6. Scrambling with matrix black holes

    NASA Astrophysics Data System (ADS)

    Brady, Lucas; Sahakian, Vatche

    2013-08-01

    If black holes are not to be dreaded sinks of information but rather fully described by unitary evolution, they must scramble in-falling data and eventually leak it through Hawking radiation. Sekino and Susskind have conjectured that black holes are fast scramblers; they generate entanglement at a remarkably efficient rate, with the characteristic time scaling logarithmically with the entropy. In this work, we focus on Matrix theory—M-theory in the light-cone frame—and directly probe the conjecture. We develop a concrete test bed for quantum gravity using the fermionic variables of Matrix theory and show that the problem becomes that of chains of qubits with an intricate network of interactions. We demonstrate that the black hole system evolves much like a Brownian quantum circuit, with strong indications that it is indeed a fast scrambler. We also analyze the Berenstein-Maldacena-Nastase model and reach the same tentative conclusion.

  7. Bright vigorous winds as signposts of supermassive black hole birth

    NASA Astrophysics Data System (ADS)

    Fiacconi, Davide; Rossi, Elena M.

    2016-01-01

    The formation of supermassive black holes is still an outstanding question. In the quasi-star scenario, black hole seeds experience an initial super-Eddington growth, that in less than a million years may leave a 104-105 M⊙ black hole at the centre of a protogalaxy at z ˜ 20-10. Super-Eddington accretion, however, may be accompanied by vigorous mass-loss that can limit the amount of mass that reaches the black hole. In this paper, we critically assess the impact of radiative driven winds, launched from the surface of the massive envelopes from which the black hole accretes. Solving the full wind equations coupled with the hydrostatic structure of the envelope, we find mass outflows with rates between a few tens and 104 M⊙ yr-1, mainly powered by advection luminosity within the outflow. We therefore confirm the claim by Dotan et al. that mass losses can severely affect the black hole seed early growth within a quasi-star. In particular, seeds with mass >104 M⊙ can only form within mass reservoirs ≳107 M⊙, unless they are refilled at huge rates (≳100 M⊙ yr-1). This may imply that only very massive haloes (>109 M⊙) at those redshifts can harbour massive seeds. Contrary to previous claims, these winds are expected to be relatively bright (1044-1047 erg s-1), blue (Teff ˜ 8000 K) objects, that while eluding the Hubble Space Telescope, could be observed by the James Webb Space Telescope.

  8. 7 CFR 201.58a - Indistinguishable seeds.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... ultraviolet light. Editorial Note: For Federal Register citations affecting § 201.58a, see the List of CFR... Brown. (4) Brown. (5) Brown Black. (d) Soybean. In determining the varietal purity, the peroxidase test... hydrogen peroxide, record the seed coat as peroxidase positive (high peroxidase activity) indicated by...

  9. 7 CFR 201.58a - Indistinguishable seeds.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... ultraviolet light. Editorial Note: For Federal Register citations affecting § 201.58a, see the List of CFR... Brown. (4) Brown. (5) Brown Black. (d) Soybean. In determining the varietal purity, the peroxidase test... hydrogen peroxide, record the seed coat as peroxidase positive (high peroxidase activity) indicated by...

  10. 7 CFR 201.58a - Indistinguishable seeds.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... ultraviolet light. Editorial Note: For Federal Register citations affecting § 201.58a, see the List of CFR... Brown. (4) Brown. (5) Brown Black. (d) Soybean. In determining the varietal purity, the peroxidase test... hydrogen peroxide, record the seed coat as peroxidase positive (high peroxidase activity) indicated by...

  11. Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Although modern soybean cultivars feature yellow seed coats, with the only color variation found at the hila, the ancestral condition is black seed coats. Both seed coat and hila coloration are due to the presence of phenylpropanoid pathway derivatives, principally anthocyanins. The genetics of soyb...

  12. Black Hole Safari: Tracking Populations and Hunting Big Game

    NASA Astrophysics Data System (ADS)

    McConnell, N. J.

    2013-10-01

    Understanding the physical connection, or lack thereof, between the growth of galaxies and supermassive black holes is a key challenge in extragalactic astronomy. Dynamical studies of nearby galaxies are building a census of black hole masses across a broad range of galaxy types and uncovering statistical correlations between galaxy bulge properties and black hole masses. These local correlations provide a baseline for studying galaxies and black holes at higher redshifts. Recent measurements have probed the extremes of the supermassive black hole population and introduced surprises that challenge simple models of black hole and galaxy co-evolution. Future advances in the quality and quantity of dynamical black hole mass measurements will shed light upon the growth of massive galaxies and black holes in different cosmic environments.

  13. The innermost extremes of black hole accretion

    NASA Astrophysics Data System (ADS)

    Fabian, A. C.

    2016-05-01

    The inner 20 gravitational radii around the black hole at the centre of luminous active galactic nuclei and stellar mass black hole binaries are now being routinely mapped by X-ray spectral-timing techniques. Spectral blurring and reverberation of the reflection spectrum are key tools in this work. In the most extreme AGN cases with high black hole spin, when the source appears in a low state, observations probe the region within 1 gravitational radius of the event horizon. The location, size, and operation of the corona which generates the power-law X-ray continuum is also being revealed.

  14. Hot seeding using large Y-123 seeds

    NASA Astrophysics Data System (ADS)

    Scruggs, S. J.; Putman, P. T.; Zhou, Y. X.; Fang, H.; Salama, K.

    2006-07-01

    There are several motivations for increasing the diameter of melt textured single domain discs. The maximum magnetic field produced by a trapped field magnet is proportional to the radius of the sample. Furthermore, the availability of trapped field magnets with large diameter could enable their use in applications that have traditionally been considered to require wound electromagnets, such as beam bending magnets for particle accelerators and electric propulsion. We have investigated the possibility of using large area epitaxial growth instead of the conventional point nucleation growth mechanism. This process involves the use of large Y123 seeds for the purpose of increasing the maximum achievable Y123 single domain size. The hot seeding technique using large Y-123 seeds was employed to seed Y-123 samples. Trapped field measurements indicate that single domain samples were indeed grown by this technique. Microstructural evaluation indicates that growth can be characterized by a rapid nucleation followed by the usual peritectic grain growth which occurs when large seeds are used. Critical temperature measurements show that no local Tc suppression occurs in the vicinity of the seed. This work supports the suggestion of using an iterative method for increasing the size of Y-123 single domains that can be grown.

  15. Kerr black holes as particle accelerators to arbitrarily high energy.

    PubMed

    Bañados, Máximo; Silk, Joseph; West, Stephen M

    2009-09-11

    We show that intermediate mass black holes conjectured to be the early precursors of supermassive black holes and surrounded by relic cold dark matter density spikes can act as particle accelerators with collisions, in principle, at arbitrarily high center-of-mass energies in the case of Kerr black holes. While the ejecta from such interactions will be highly redshifted, we may anticipate the possibility of a unique probe of Planck-scale physics. PMID:19792361

  16. From the first stars to the first black holes

    NASA Astrophysics Data System (ADS)

    Valiante, Rosa; Schneider, Raffaella; Volonteri, Marta; Omukai, Kazuyuki

    2016-04-01

    The growth of the first supermassive black holes (SMBHs) at z > 6 is still a major challenge for theoretical models. If it starts from black hole (BH) remnants of Population III stars (light seeds with mass ˜100 M⊙), it requires super-Eddington accretion. An alternative route is to start from heavy seeds formed by the direct collapse of gas on to an ˜105 M⊙ BH. Here we investigate the relative role of light and heavy seeds as BH progenitors of the first SMBHs. We use the cosmological, data constrained semi-analytic model GAMETE/QSODUST to simulate several independent merger histories of z > 6 quasars. Using physically motivated prescriptions to form light and heavy seeds in the progenitor galaxies, we find that the formation of a few heavy seeds (between 3 and 30 in our reference model) enables the Eddington-limited growth of SMBHs at z > 6. This conclusion depends sensitively on the interplay between chemical, radiative and mechanical feedback effects, which easily erase the conditions that allow the suppression of gas cooling in the low-metallicity gas (Z < Zcr and JLW > Jcr). We find that heavy seeds cannot form if dust cooling triggers gas fragmentation above a critical dust-to-gas mass ratio (D ≥ D_cr). In addition, the relative importance of light and heavy seeds depends on the adopted mass range for light seeds, as this dramatically affects the history of cold gas along the merger tree, by both SN- and AGN-driven winds.

  17. A role for seed storage proteins in Arabidopsis seed longevity

    PubMed Central

    Nguyen, Thu-Phuong; Cueff, Gwendal; Hegedus, Dwayne D; Rajjou, Loïc; Bentsink, Leónie

    2015-01-01

    Proteomics approaches have been a useful tool for determining the biological roles and functions of individual proteins and identifying the molecular mechanisms that govern seed germination, vigour and viability in response to ageing. In this work the dry seed proteome of four Arabidopsis thaliana genotypes, that carry introgression fragments at the position of seed longevity quantitative trait loci and as a result display different levels of seed longevity, was investigated. Seeds at two physiological states, after-ripened seeds that had the full germination ability and aged (stored) seeds of which the germination ability was severely reduced, were compared. Aged dry seed proteomes were markedly different from the after-ripened and reflected the seed longevity level of the four genotypes, despite the fact that dry seeds are metabolically quiescent. Results confirmed the role of antioxidant systems, notably vitamin E, and indicated that protection and maintenance of the translation machinery and energy pathways are essential for seed longevity. Moreover, a new role for seed storage proteins (SSPs) was identified in dry seeds during ageing. Cruciferins (CRUs) are the most abundant SSPs in Arabidopsis and seeds of a triple mutant for three CRU isoforms (crua crub cruc) were more sensitive to artificial ageing and their seed proteins were highly oxidized compared with wild-type seeds. These results confirm that oxidation is involved in seed deterioration and that SSPs buffer the seed from oxidative stress, thus protecting important proteins required for seed germination and seedling formation. PMID:26184996

  18. Volatile fingerprint of Brazilian defective coffee seeds: corroboration of potential marker compounds and identification of new low quality indicators.

    PubMed

    Toci, Aline T; Farah, Adriana

    2014-06-15

    In the present work, the volatile profiles of green and roasted Brazilian defective coffee seeds and their controls were characterised, totalling 159 compounds. Overall, defective seeds showed higher number and concentration of volatile compounds compared to those of control seeds, especially pyrazines, pyrroles and phenols. Corroborating our previous results, butyrolactone and hexanoic acid, previously considered as potential defective seeds' markers, were observed only in raw and roasted defective seeds, respectively, and not in control seeds. New compounds were suggested as potential defective seeds' markers: hexanoic acid (for raw and roasted defective seeds in general), butyrolactone (for raw defective seeds in general), and 3-ethyl-2-methyl-1,3-hexadiene (for raw black seeds); β-linalool and 2-butyl-3,5-dimethylpyrazine (for roasted defective seeds in general), and 2-pentylfuran (for roasted black seeds). Additional compounds suggested as low quality indicators were 2,3,5,6-tetramethylpyrazine,2,3-butanediol and 4-ethylguaiacol, β-linalool, 2-,3-dimethylbutyl butanoate, 2-phenylethyl acetate, 2,3-butanedione, hexanedioic acid, guaiacol, 2,3-dihydro-2-methyl-1H-benzopyrrol, 3-methylpiperidine, 2-pentylpiperidine, 3-octen-2-one, 2-octenal, 2-pentylfuran and 2-butyl-3-methylpyrazine. PMID:24491734

  19. A quick SEED tutorial

    USGS Publications Warehouse

    Ringler, Adam; Evans, John R.

    2015-01-01

    A number of different government-funded seismic data centers offer free open-access data (e.g., U.S. Geological Survey, National Earthquake Information Center, the Incorporated Research Institutions for Seismology (IRIS), and Data Management System), which can be freely downloaded and shared among different members of the community (Lay, 2009). To efficiently share data, it is important that different data providers follow a common format. The Standard for the Exchange of Earthquake Data (SEED) provides one such format for storing seismic and other geophysical data. The SEED format is widely used in earthquake seismology; however, SEED and its structure can be difficult for many first-time users (ourselves included). Below is a quick tutorial that outlines the basic structure of SEED format. This write-up is in no way intended to replace the comprehensive SEED manual (Ahern et al., 2009), and instead of going into the details of any specific part of the SEED format we refer the reader to the manual for additional details. The goal of this write-up is to succinctly explain the basic structure of SEED format as well as the associated jargon, as most commonly used now, in a colloquial way so that novice users of SEED can become more familiar with the format and its application quickly. Our goal is to give the reader the necessary background so that when problems or questions about SEED format arise they will have some understanding of where they should look for more details or from where the problem might be stemming. As a secondary goal, we hope to help the reader become familiar with the SEED manual (Ahern et al., 2009), which contains detailed information about all aspects of the SEED format.

  20. Charged rotating dilaton black holes with Kaluza-Klein asymptotics

    NASA Astrophysics Data System (ADS)

    Knoll, Christian; Nedkova, Petya

    2016-03-01

    We construct a class of stationary and axisymmetric solutions to the five-dimensional Einstein-Maxwell-dilaton gravity, which describe configurations of charged rotating black objects with Kaluza-Klein asymptotics. The solutions are constructed by uplifting a vacuum seed solution to six dimensions, performing a boost and a subsequent circle reduction. We investigate the physical properties of the charged solutions and obtain their general relations to the properties of the vacuum seed. We also derive the gyromagnetic ratio and the Smarr-like relations. As particular cases, we study three solutions, which describe a charged rotating black string, a charged rotating black ring on Kaluza-Klein bubbles, and a superposition of two black holes and a Kaluza-Klein bubble.

  1. Examining Children's Models of Seed.

    ERIC Educational Resources Information Center

    Jewell, Natalie

    2002-01-01

    Reports research that examines children's models of seed. Explores the conceptions held by children (N=75) of germination and seed formation. Concludes that children hold a restricted meaning for the term 'seed'. (DDR)

  2. Characterization of two DNA probes specific for Serpulina hyodysenteriae.

    PubMed Central

    Sotiropoulos, C; Smith, S C; Coloe, P J

    1993-01-01

    Two DNA probes, one 1.1- and one 0.75-kb probe, specific for Serpulina hyodysenteriae were isolated from a genomic library generated from virulent S. hyodysenteriae 5380. These probes are highly specific and react with all S. hyodysenteriae strains tested. Under stringent conditions, the DNA probes did not react with the nonpathogenic species Serpulina innocens or with other species of enteric bacteria, including Escherichia coli. Both probes are able to detect S. hyodysenteriae in colony blot hybridizations, and when applied to fecal specimens, they can detect 10(4) S. hyodysenteriae cells in 0.1 g of seeded fecal matter. Both probes can detect S. hyodysenteriae in fecal specimens from swine with clinical signs of swine dysentery after experimental challenge and from swine from a herd with an acute outbreak of swine dysentery. These probes have application as a diagnostic tool in veterinary microbiology. Images PMID:8349750

  3. A seed coat-specific promoter for canola.

    PubMed

    El-Mezawy, Aliaa; Wu, Limin; Shah, Saleh

    2009-12-01

    The canola industry generates more than $11 billion of yearly income to the Canadian economy. One problem of meal quality is the dark polyphenolic pigments that accumulate in the seed coat. Seed coat-specific promoters are a pre-requisite to regulate the genes involved in seed coat development and metabolism. The beta-glucuronidase (GUS) reporter gene was used to test an Arabidopsis promoter in developing and mature seeds of canola (Brassica napus). The promoter tested is the regulatory region of the laccase gene (AtLAC15) from Arabidopsis thaliana. The AtLAC15 promoter::GUS construct was inserted into canola double haploid line DH12075 using Agrobacterium-mediated transformation. Southern blot analysis using a 536 bp GUS probe showed variation among the transformed plants in the T-DNA copy numbers and the position of the insertion in their genomes. Histochemical assay of the GUS enzyme in different tissues (roots, leaves, stem, pollen grains, flowers, siliques, embryos and seed coats) showed ascending GUS activity only in the seed coat from 10 days after pollination (DAP) to the fully mature stage (35 DAP). GUS stain was observed in the mucilage cell layer, in the outer integument layer of the seed coat but not in the inner integument. The AtLAC15 promoter exhibited a specificity and expression level that is useful as a seed coat-specific promoter for canola. PMID:19690805

  4. GROWING SEEDS, TEACHER'S GUIDE.

    ERIC Educational Resources Information Center

    Elementary Science Study, Newton, MA.

    THIS TEACHER'S GUIDE IS DESIGNED FOR USE WITH AN ELEMENTARY SCIENCE STUDY UNIT, "GROWING SEEDS," IN WHICH SUCH BASIC SCIENCE SKILLS AND PROCESSES AS MEASUREMENT, OBSERVATION, AND HYPOTHESIS FORMATION ARE INTRODUCED THROUGH STUDENT ACTIVITIES INVOLVING SEEDS, GERMINATION, AND SEEDLING GROWTH. THE MATERIALS WERE DEVELOPED FOR USE IN ELEMENTARY…

  5. [Quality classification criteria of Paeonia suffruticosa seeds].

    PubMed

    Cao, Ya-yue; Zhu, Zai-biao; Guo, Qiao-sheng; Liu, Li; Wang, Chang-lin

    2015-02-01

    In order to establish the quality classification criteria of Paeonia suffruticosa seeds, thirty-one batches of P. suffruticosa seeds from different provenances were selected. The seed rooting rate, seed germination rate, seed purity, seed viability, 1,000-seed weight and moisture content were determined and analyzed through SPSS 20.0 software. Seed rooting rate, seed germination rate and seed purity were selected as the main index for classification, while 1,000-seed weight, seed viability and moisture content could be used as important references. The seed quality grading of P. suffruticosa was set as three grades. The seed quality of each grade should meet following requirements: For the first grade seeds, seed rooting rate ≥ 80%, seed germination rate ≥ 80%, seed purity ≥ 90%, seed viability ≥ 80%, 1,000-seed weight ≥ 250 g, moisture content, ≤ 10. For the second grade seeds, seed rooting rate ≥ 50%, seed germination rate ≥ 60%, seed purity ≥ 70%, seed viability ≥ 75%, 1,000-seed weight ≥ 225 g, moisture content ≤ 10. For the third grade seeds, seed rooting rate ≥ 20%, seed germination rate ≥ 45%, seed purity ≥ 60%, seed viability ≥ 45%, 1,000-seed weight ≥ 205 g, moisture content ≤ 10. The quality classification criteria of P. suffruticosa seeds have been initially established. PMID:26137680

  6. How black holes stay black

    NASA Astrophysics Data System (ADS)

    Genzel, Reinhard

    1998-01-01

    The dimness of the black holes located at the center of galaxies surprises astrophysicists, but a possible explanation has been found in the behavior of the plasma they consume. In a hot accretion flow, the gas is ionized to form a plasma. The heavy ions carry most of the mass, and thus of the energy, whereas the electrons produce most of the radiation. But, crucially, in a low-density flow the temperatures of the ions and of the electrons may decouple. Consequently, most of the gravitational energy would be viscously converted into thermal energy of the ions and not radiated away by the electrons. Instead, the gravitational energy is carried with the flow across the event horizon of the black hole. Such a flow leads to a low radiation efficiency even in a highly dissipative accretion disk.

  7. Smoking Cessation among Blacks.

    ERIC Educational Resources Information Center

    Stotts, R. Craig; And Others

    1991-01-01

    Lung cancer is a serious health problem among blacks, with a mortality rate of 119 per 100,000 black males, compared to 81 per 100,000 for white males. Smoking cessation efforts are most successful when tailored to the black community, using black community networks and broadcast media for black audiences. (SLD)

  8. On Responsible Black Leadership.

    ERIC Educational Resources Information Center

    Farrakhan, Louis

    1985-01-01

    Black leaders in the United States must unite among themselves and work for the Black community. Black elected officials should know the will of their constituency before taking a particular stance. Finally, Black leaders should ally themselves with international leaders friendly to the cause of American Blacks. (GC)

  9. Contemporary Black Theatre.

    ERIC Educational Resources Information Center

    Thomas, Pearl

    The distinguishable black theatre in America, mirroring a distinguishable black experience, is an artistic product which demands audience involvement. Both the Afro-American oral tradition and the art of gesture are integral aspects of black theatre. In addition, the tragedy found black theatre is not tragedy in the classic sense, as blacks feel…

  10. ERRATUM: In vivo evaluation of a neural stem cell-seeded prosthesis In vivo evaluation of a neural stem cell-seeded prosthesis

    NASA Astrophysics Data System (ADS)

    Purcell, E. K.; Seymour, J. P.; Yandamuri, S.; Kipke, D. R.

    2009-08-01

    In the published article, an error was made in figure 5. Specifically, the three-month, NSC-seeded image is a duplicate of the six-week image, and the one-day, probe alone image is a duplicate of the three-month image. The corrected figure is reproduced below. Figure 5 Figure 5. Glial encapsulation of each probe condition over the 3 month time course. Ox-42 labeled microglia and GFAP labeled astrocytes are shown. Images are taken from probes implanted in the same animal at each time point. NSC seeding was associated with reduced non-neuronal density at 1 day post-implantation in comparison to alginate coated probes and at the 1 week time point in comparison to untreated probes (P < 0.001). Glial activation is at its overall peak 1 week after insertion. A thin encapsulation layer surrounds probes at the 6 week and 3 month time points, with NSC-seeded probes having the greatest surrounding non-neuronal density P < 0.001). Interestingly, microglia appeared to have a ramified, or `surveilling', morphology surrounding a neural stem cell-alginate probe initially, whereas activated cells with an amoeboid structure were found near an alginate probe in the same hemisphere of one animal (left panels).