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Sample records for proteus mirabilis pseudomonas

  1. [Peculiarities of Proteus mirabilis extracellular metalloproteinase biosynthesis].

    PubMed

    Zamaliutdinova, N M; Sharipova, M R; Bogomol'naia, L M; Bozhokina, E S; Mardanova, A M

    2015-01-01

    Biosynthesis of metalloproteinase by the Proteus mirabilis 5127-1 strain on different media and the influence of glucose and urea on biosynthesis were studied. It was found that the P. mirabilis 5127-1 bacteria secretes metalloproteinase in the medium in two isoforms (52 and 50 kDa). It was established that proteinase synthesis is completely suppressed during the growth of bacteria on synthetic media, as well as in the presence of LB glucose in the medium. It was demonstrated that addition of urea in the medium results in an increase of the culture productivity in the proteinase synthesis. Maximal culture productivity in the proteinase synthesis was found in the medium with natural urine. During the growth of bacteria on artificial urine, proteinase appeared in the medium only after 12 hours of growth as a single isoform. PMID:25872397

  2. Capsule structure of Proteus mirabilis (ATCC 49565).

    PubMed Central

    Beynon, L M; Dumanski, A J; McLean, R J; MacLean, L L; Richards, J C; Perry, M B

    1992-01-01

    Proteus mirabilis 2573 (ATCC 49565) produces an acidic capsular polysaccharide which was shown from glycose analysis, carboxyl reduction, methylation, periodate oxidation, and the application of one dimensional and two-dimensional high-resolution nuclear magnetic resonance techniques to be a high-molecular-weight polymer of branched trisaccharide units composed of 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine), 2-acetamido-2,6-dideoxy-L-galactose (N-acetyl-L-fucosamine), and D-glucuronic acid, having the structure: [formula: see text] P. mirabilis 2573 also produces an O:6 serotype lipopolysaccharide in which the O-chain component has the same structure as the homologous capsular polysaccharide. This is the first report of a defined capsular polysaccharide in this bacterial genus. PMID:1551839

  3. Radial and Spiral Stream Formation in Proteus mirabilis Colonies

    E-print Network

    Xue, Chuan

    The enteric bacterium Proteus mirabilis, which is a pathogen that forms biofilms in vivo, can swarm over hard surfaces and form a variety of spatial patterns in colonies. Colony formation involves two distinct cell types: ...

  4. Proteus mirabilis interkingdom swarming signals attract blow flies

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Flies transport specific bacteria with their larvae which provides a wider range of nutrients for those bacteria. Our hypothesis was that this symbiotic interaction may depend on interkingdom signaling. We obtained Proteus mirabilis from the salivary glands of the blow fly Lucilia sericat. This s...

  5. Proteus mirabilis and rheumatoid arthritis: no association with the disease.

    PubMed

    Chandrashekara, S; Ramesh, M N; Shobha, A; Saravanan, Y; Vadiraja, H S; Navaneeth, B V; Sandhya Belwadi, M R

    2003-09-01

    Proteus mirabilis(PM) is implicated in different studies in the pathogenesis of rheumatoid arthritis (RA) because of the structural homogeneity of its haemolysin B precursor with EQRRAA sequences in DRB 1 haplotype. The aim of the study was to compare the levels of antibodies specific to PM in the sera of patients with RA and healthy controls in our population. Serum samples from 78 consecutive RA patients and 75 healthy controls were analysed for the presence of IgG isotype and total immunoglobulins (IgG+IgA+IgM) against PM using enzyme-linked immunosorbent assay (ELISA) with two kinds of antigen preparation, whole bacteria and SDS-lysed bacterial extract. There was no significant increase in the concentrations of anti- Proteus antibodies (APA) in patients with RA compared to healthy controls in our population, when SDS-lysed bacterial extract or whole bacteria were used as antigen. The APA levels did not correlate with serum CRP levels. Infection with P. mirabilis is found to have no pathological or aggravating role in RA. PMID:14505214

  6. Proteus mirabilis and rheumatoid arthritis: no association with the disease.

    PubMed

    Chandrashekara, S; Ramesh, M N; Shobha, A; Saravanan, Y; Vadiraja, H S; Navaneeth, B V; Sandhya Belwadi, M R

    2003-10-01

    Proteus mirabilis (PM) is implicated in different studies in the pathogenesis of rheumatoid arthritis (RA) because of the structural homogeneity of its haemolysin B precursor with EQRRAA sequences in DRB 1 haplotype. The aim of the study was to compare the levels of antibodies specific to PM in the sera of patients with RA and healthy controls in our population. Serum samples from 78 consecutive RA patients and 75 healthy controls were analysed for the presence of IgG isotype and total immunoglobulins (IgG + IgA + IgM) against PM using enzyme-linked immunosorbent assay (ELISA) with two kinds of antigen preparations, whole bacteria and SDS-lysed bacterial extract. There was no significant increase in the concentrations of anti- Proteus antibodies (APA) in patients with RA compared to healthy controls in our population, when SDS-lysed bacterial extract or whole bacteria were used as antigen. The APA levels did not correlate with serum CRP levels. We conclude that P. mirabilis has no pathological or aggravating role in RA. PMID:14576988

  7. New Aspects of RpoE in Uropathogenic Proteus mirabilis

    PubMed Central

    Liu, Ming-Che; Kuo, Kuan-Ting; Chien, Hsiung-Fei; Tsai, Yi-Lin

    2014-01-01

    Proteus mirabilis is a common human pathogen causing recurrent or persistent urinary tract infections (UTIs). The underlying mechanisms for P. mirabilis to establish UTIs are not fully elucidated. In this study, we showed that loss of the sigma factor E (RpoE), mediating extracytoplasmic stress responses, decreased fimbria expression, survival in macrophages, cell invasion, and colonization in mice but increased the interleukin-8 (IL-8) expression of urothelial cells and swarming motility. This is the first study to demonstrate that RpoE modulated expression of MR/P fimbriae by regulating mrpI, a gene encoding a recombinase controlling the orientation of MR/P fimbria promoter. By real-time reverse transcription-PCR, we found that the IL-8 mRNA amount of urothelial cells was induced significantly by lipopolysaccharides extracted from rpoE mutant but not from the wild type. These RpoE-associated virulence factors should be coordinately expressed to enhance the fitness of P. mirabilis in the host, including the avoidance of immune attacks. Accordingly, rpoE mutant-infected mice displayed more immune cell infiltration in bladders and kidneys during early stages of infection, and the rpoE mutant had a dramatically impaired ability of colonization. Moreover, it is noteworthy that urea (the major component in urine) and polymyxin B (a cationic antimicrobial peptide) can induce expression of rpoE by the reporter assay, suggesting that RpoE might be activated in the urinary tract. Altogether, our results indicate that RpoE is important in sensing environmental cues of the urinary tract and subsequently triggering the expression of virulence factors, which are associated with the fitness of P. mirabilis, to build up a UTI. PMID:25547796

  8. Characterization and spectral properties of Proteus mirabilis PR catalase.

    PubMed

    Jouve, H M; Gaillard, J; Pelmont, J

    1984-10-01

    Purified catalase from a peroxide-resistant mutant (PR) of Proteus mirabilis displayed great similarities with the bovine liver catalase on the basis of its amino acid composition, content in prosthetic groups, and spectroscopic data. The bacterial enzyme was found to have 2.6 +/- 0.2 mol of protoheme IX per tetramer, with an equivalent amount of titrable iron atoms. The optical absorption of P. mirabilis PR catalase in the presence of various anionic species (cyanide, azide, formate) was examined. The dissociation constant of the formate-enzyme complex was determined as 60 +/- 2 mM at pH 7.5. Inhibition and spectral shifts induced by some thiol compounds were very similar to those reported with mammalian catalase. The electron paramagnetic resonance (EPR) spectra (at 9 GHz and 6 K) of bacterial catalase and its various complexes were reported. Two major different rhombic high-spin ferric signals could be seen in the g = 6 region, using either the pure enzyme or the cell crude extract. The balance between the two rhombic forms was reversibly altered by pH. Various changes in rhombicity were also observed after binding with anionic ligands. The EPR spectrum (at 40 K) of nitrosyl ferrous catalase was very similar to reported data with horse liver catalase. PMID:6095975

  9. Multidrug-Resistant Proteus mirabilis Isolated From Newly Weaned Infant Rhesus Monkeys and Ferrets

    PubMed Central

    Yu, Wenhai; He, Zhanlong; Huang, Fen

    2015-01-01

    Background: Proteus mirabilis is an important uropathogen that causes complicated Urinary Tract Infection (UTI) and induces diarrhea in infants. Objectives: This study aimed to investigate P. mirabilis infection in newly weaned infant rhesus monkeys (Macaca mulatta) and ferrets (Mustela putorius furo) with diarrhea. Materials and Methods: Stool samples were collected from 74 rhesus monkeys and 12 ferrets with diarrhea. Proteus mirabilis was isolated from the samples through Polymerase Chain Reaction. The isolated P. mirabilis was subjected to antimicrobial susceptibility tests. Results: Seven (7/74, 9.5%) and four (4/12, 30%) P. mirabilis strains were detected in the stool samples collected from the monkeys and ferrets, respectively. Sequence analyses showed that the isolated P. mirabilis was closely related to P. mirabilis strain HI4320, which was isolated from the urine of a patient with a long-term indwelling urinary catheter. In addition, the isolates demonstrated multidrug resistance. Conclusions: Rhesus monkeys and ferrets are susceptible to P. mirabilis, making them useful as animal models for future studies on the mechanism of P. mirabilis-induced UTI and its corresponding treatment. PMID:26301055

  10. Arbitrarily primed polymerase chain reaction provides rapid differentiation of Proteus mirabilis isolates from a pediatric hospital.

    PubMed Central

    Bingen, E; Boissinot, C; Desjardins, P; Cave, H; Brahimi, N; Lambert-Zechovsky, N; Denamur, E; Blot, P; Elion, J

    1993-01-01

    During a systematic survey, maternal carriage of Proteus mirabilis was found over a 25-day period in 18 pregnant women admitted to the delivery ward of our hospital maternity. Five neonates born to these mothers were found to be colonized with P. mirabilis. We report here on the use of DNA fingerprinting by the arbitrarily primed polymerase chain reaction technique (AP-PCR) for the epidemiological investigation of this sudden outbreak. This approach was compared with the analysis of restriction fragment length polymorphisms of ribosomal DNA regions (ribotyping). Results of the AP-PCR and of ribotyping were in complete agreement in showing the genetic unrelatedness of the isolates obtained from each mother. Moreover, the results showed mother-to-infant vertical transmission of P. mirabilis in the neonates. AP-PCR is a rapid and discriminative method which seems particularly well suited to the epidemiological study of P. mirabilis. Images PMID:8099079

  11. Eugenol alters the integrity of cell membrane and acts against the nosocomial pathogen Proteus mirabilis.

    PubMed

    Devi, K Pandima; Sakthivel, R; Nisha, S Arif; Suganthy, N; Pandian, S Karutha

    2013-03-01

    Eugenol, a member of the phenylpropanoids class of chemical compounds, is a clear to pale yellow oily liquid extracted from certain essential oils especially from clove oil, nutmeg, cinnamon, and bay leaf. The antibacterial activity of eugenol and its mechanism of bactericidal action against Proteus mirabilis were evaluated. Treatment with eugenol at their minimum inhibitory concentration [0.125 % (v/v)] and minimum bactericidal concentration [0.25 % (v/v)] reduced the viability and resulted in complete inhibition of P. mirabilis. A strong bactericidal effect on P. mirabilis was also evident, as eugenol inactivated the bacterial population within 30 min exposure. Chemo-attractant property and the observance of highest antibacterial activity at alkaline pH suggest that eugenol can work more effectively when given in vivo. Eugenol inhibits the virulence factors produced by P. mirabilis as observed by swimming motility, swarming behavior and urease activity. It interacts with cellular membrane of P. mirabilis and makes it highly permeable, forming nonspecific pores on plasma membrane, which in turn directs the release of 260 nm absorbing materials and uptake of more crystal violet from the medium into the cells. SDS-polyacrylamide gel, scanning electron microscopy and Fourier transform infrared analysis further proves the disruptive action of eugenol on the plasma membrane of P. mirabilis. The findings reveal that eugenol shows an excellent bactericidal activity against P. mirabilis by altering the integrity of cell membrane. PMID:23444040

  12. Transcriptome of Proteus mirabilis in the murine urinary tract: virulence and nitrogen assimilation gene expression.

    PubMed

    Pearson, Melanie M; Yep, Alejandra; Smith, Sara N; Mobley, Harry L T

    2011-07-01

    The enteric bacterium Proteus mirabilis is a common cause of complicated urinary tract infections. In this study, microarrays were used to analyze P. mirabilis gene expression in vivo from experimentally infected mice. Urine was collected at 1, 3, and 7 days postinfection, and RNA was isolated from bacteria in the urine for transcriptional analysis. Across nine microarrays, 471 genes were upregulated and 82 were downregulated in vivo compared to in vitro broth culture. Genes upregulated in vivo encoded mannose-resistant Proteus-like (MR/P) fimbriae, urease, iron uptake systems, amino acid and peptide transporters, pyruvate metabolism enzymes, and a portion of the tricarboxylic acid (TCA) cycle enzymes. Flagella were downregulated. Ammonia assimilation gene glnA (glutamine synthetase) was repressed in vivo, while gdhA (glutamate dehydrogenase) was upregulated in vivo. Contrary to our expectations, ammonia availability due to urease activity in P. mirabilis did not drive this gene expression. A gdhA mutant was growth deficient in minimal medium with citrate as the sole carbon source, and loss of gdhA resulted in a significant fitness defect in the mouse model of urinary tract infection. Unlike Escherichia coli, which represses gdhA and upregulates glnA in vivo and cannot utilize citrate, the data suggest that P. mirabilis uses glutamate dehydrogenase to monitor carbon-nitrogen balance, and this ability contributes to the pathogenic potential of P. mirabilis in the urinary tract. PMID:21505083

  13. Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase

    PubMed Central

    Aquilini, Eleonora; Merino, Susana; Knirel, Yuriy A.; Regué, Miguel; Tomás, Juan M.

    2014-01-01

    By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains. PMID:24756091

  14. Molecular detection of HpmA and HlyA hemolysin of uropathogenic Proteus mirabilis.

    PubMed

    Cestari, Silvia Emanoele; Ludovico, Marilucia Santos; Martins, Fernando Henrique; da Rocha, Sérgio Paulo Dejato; Elias, Waldir Pereira; Pelayo, Jacinta Sanchez

    2013-12-01

    Urinary tract infection (UTI) is one of the bacterial infections frequently documented in humans. Proteus mirabilis is associated with UTI mainly in individuals with urinary tract abnormality or related with vesicular catheterism and it can be difficult to treat because of the formation of stones in the bladder and kidneys. These stones are formed due to the presence of urease synthesized by the bacteria. Another important factor is that P. mirabilis produces hemolysin HpmA, used by the bacteria to damage the kidney tissues. Proteus spp. samples can also express HlyA hemolysin, similar to that found in Escherichia coli. A total of 211 uropathogenic P. mirabilis isolates were analyzed to detect the presence of the hpmA and hpmB genes by the techniques of polymerase chain reaction (PCR) and dot blot and hlyA by PCR. The hpmA and hpmB genes were expressed by the RT-PCR technique and two P. mirabilis isolates were sequenced for the hpmA and hpmB genes. The presence of the hpmA and hpmB genes was confirmed by PCR in 205 (97.15 %) of the 211 isolates. The dot blot confirmed the presence of the hpmA and hpmB genes in the isolates that did not amplify in the PCR. None of the isolates studied presented the hlyA gene. The hpmA and hpmB genes that were sequenced presented 98 % identity with the same genes of the HI4320 P. mirabilis sample. This study showed that the PCR technique has good sensitivity for detecting the hpmA and hpmB genes of P. mirabilis. PMID:23884594

  15. Proteus mirabilis biofilm - Qualitative and quantitative colorimetric methods-based evaluation

    PubMed Central

    Kwiecinska-Piróg, Joanna; Bogiel, Tomasz; Skowron, Krzysztof; Wieckowska, Ewa; Gospodarek, Eugenia

    2014-01-01

    Proteus mirabilis strains ability to form biofilm is a current topic of a number of research worldwide. In this study the biofilm formation of P. mirabilis strains derived from urine of the catheterized and non-catheterized patients has been investigated. A total number of 39 P. mirabilis strains isolated from the urine samples of the patients of dr Antoni Jurasz University Hospital No. 1 in Bydgoszcz clinics between 2011 and 2012 was used. Biofilm formation was evaluated using two independent quantitative and qualitative methods with TTC (2,3,5-triphenyl-tetrazolium chloride) and CV (crystal violet) application. The obtained results confirmed biofilm formation by all the examined strains, except quantitative method with TTC, in which 7.7% of the strains did not have this ability. It was shown that P. mirabilis rods have the ability to form biofilm on the surfaces of both biomaterials applied, polystyrene and polyvinyl chloride (Nelaton catheters). The differences in ability to form biofilm observed between P. mirabilis strains derived from the urine of the catheterized and non-catheterized patients were not statistically significant. PMID:25763050

  16. Allicin from garlic inhibits the biofilm formation and urease activity of Proteus mirabilis in vitro.

    PubMed

    Ranjbar-Omid, Mahsa; Arzanlou, Mohsen; Amani, Mojtaba; Shokri Al-Hashem, Seyyedeh Khadijeh; Amir Mozafari, Nour; Peeri Doghaheh, Hadi

    2015-05-01

    Several virulence factors contribute to the pathogenesis of Proteus mirabilis. This study determined the inhibitory effects of allicin on urease, hemolysin and biofilm of P. mirabilis ATCC 12453 and its antimicrobial activity against 20 clinical isolates of P. mirabilis. Allicin did not inhibit hemolysin, whereas it did inhibit relative urease activity in both pre-lysed (half-maximum inhibitory concentration, IC50 = 4.15 ?g) and intact cells (IC50 = 21 ?g) in a concentration-dependent manner. Allicin at sub-minimum inhibitory concentrations (2-32 ?g mL(-1)) showed no significant effects on the growth of the bacteria (P > 0.05), but it reduced biofilm development in a concentration-dependent manner (P < 0.001). A higher concentration of allicin was needed to inhibit the established biofilms. Using the microdilution technique, the MIC90 and MBC90 values of allicin against P. mirabilis isolates were determined to be 128 and 512 ?g mL(-1), respectively. The results suggest that allicin could have clinical applications in controlling P. mirabilis infections. PMID:25837813

  17. Characterization of 17 chaperone-usher fimbriae encoded by Proteus mirabilis reveals strong conservation

    PubMed Central

    Kuan, Lisa; Schaffer, Jessica N.; Zouzias, Christos D.

    2014-01-01

    Proteus mirabilis is a Gram-negative enteric bacterium that causes complicated urinary tract infections, particularly in patients with indwelling catheters. Sequencing of clinical isolate P. mirabilis HI4320 revealed the presence of 17 predicted chaperone-usher fimbrial operons. We classified these fimbriae into three groups by their genetic relationship to other chaperone-usher fimbriae. Sixteen of these fimbriae are encoded by all seven currently sequenced P. mirabilis genomes. The predicted protein sequence of the major structural subunit for 14 of these fimbriae was highly conserved (?95?% identity), whereas three other structural subunits (Fim3A, UcaA and Fim6A) were variable. Further examination of 58 clinical isolates showed that 14 of the 17 predicted major structural subunit genes of the fimbriae were present in most strains (>85?%). Transcription of the predicted major structural subunit genes for all 17 fimbriae was measured under different culture conditions designed to mimic conditions in the urinary tract. The majority of the fimbrial genes were induced during stationary phase, static culture or colony growth when compared to exponential-phase aerated culture. Major structural subunit proteins for six of these fimbriae were detected using MS of proteins sheared from the surface of broth-cultured P. mirabilis, demonstrating that this organism may produce multiple fimbriae within a single culture. The high degree of conservation of P. mirabilis fimbriae stands in contrast to uropathogenic Escherichia coli and Salmonella enterica, which exhibit greater variability in their fimbrial repertoires. These findings suggest there may be evolutionary pressure for P. mirabilis to maintain a large fimbrial arsenal. PMID:24809384

  18. Detection of KPC-2 in a Clinical Isolate of Proteus mirabilis and First Reported Description of Carbapenemase Resistance Caused by a KPC Beta-Lactamase in P. mirabilis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    An isolate of Proteus mirabilis recovered from bacterial cultures was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole cell and/or plasmid DNA recovered from the isolate using primers specific for the blaKPC carbapenemase g...

  19. Immunological Characterization of Two Major Proteins Isolated from the Outer Membrane of Proteus mirabilis

    PubMed Central

    Bub, Felizitas; Bieker, Petra; Martin, Hans H.; Nixdorff, Kathryn

    1980-01-01

    Two proteins with apparent molecular weights of 39,000 and 36,000 (Mr 39,000 and Mr 36,000, respectively) were isolated from the outer membrane of Proteus mirabilis 19. Mr 36,000 was shown to be free of detectable amounts of the Mr 39,000 protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and free of lipopolysaccharide according to gas chromatographic analyses of 3-hydroxymyristic acid content. The Mr 39,000 protein contained no detectable amount of lipopolysaccharide and only a trace of Mr 36,000. Both isolated proteins gave strong reactions in antisera produced to purified P. mirabilis 19 cell walls (outer membrane proteins in the native state). This suggested that the proteins isolated by our methods essentially retained their native configuration upon resolubilization. Antisera produced in rabbits to the isolated proteins showed strongest reactions with the homologous antigen, but some cross-reactions with the heterologous protein and with P. mirabilis 19 lipopolysaccharide were observed. These cross-reactions could be attributed to specific responses to traces of the heterologous (contaminant) proteins present in the purified proteins used as immunizing antigens. The Mr 39,000 and Mr 36,000 proteins have no major antigenic determinants in common. Reactions with P. mirabilis 19 lipopolysaccharide in antisera to the outer membrane proteins could be completely removed by absorption of the antisera with the Mr 36,000 protein. Images Fig. 1 Fig. 2 PMID:6155331

  20. Pulmonary Pneumatocele in a Pneumonia Patient Infected with Extended-Spectrum ?-Lactamase Producing Proteus mirabilis

    PubMed Central

    Ryou, Sung Hyeok; Bae, Jong Wook; Baek, Hyun Jin; Lee, Doo Hyuk; Lee, Sang Won; Choi, Gyu Ho; Han, Kyu Hyung; Kim, Se Weon; Kim, Hyunbeom

    2015-01-01

    Pulmonary pneumatoceles are air-filled thin-walled spaces within the lung and are rare in adult cases of pneumonia. We report the case of a 74-year-old male who was admitted with a cough and sputum production. He had been treated with oral dexamethasone since a brain tumorectomy 6 months prior. Contrast-enhanced computed tomography (CT) of the chest revealed a large pneumatocele in the right middle lobe and peripheral pneumonic consolidation. Bronchoalveolar lavage was performed; cultures identified extended-spectrum ?-lactamase (ESBL) producing Proteus mirabilis. A 4-week course of intravenous ertapenem was administered, and the pneumatocele with pneumonia resolved on follow-up chest CT. To the best of our knowledge, this is the first reported case of pulmonary pneumatocele caused by ESBL-producing P. mirabilis associated with pneumonia. PMID:26508927

  1. Two novel Salmonella genomic island 1 variants in Proteus mirabilis isolates from swine farms in China.

    PubMed

    Lei, Chang-Wei; Zhang, An-Yun; Liu, Bi-Hui; Wang, Hong-Ning; Yang, Li-Qin; Guan, Zhong-Bin; Xu, Chang-Wen; Zhang, Dong-Dong; Yang, Yong-Qiang

    2015-07-01

    Four different Salmonella genomic island 1 (SGI1) variants, including two novel variants, were characterized in one Salmonella enterica serovar Rissen sequence type ST1917 isolate and three Proteus mirabilis isolates from swine farms in China. One novel variant was derived from SGI1-B with the backbone gene S021 disrupted by a 12.72-kb IS26 composite transposon containing the dfrA17-aadA5 cassettes and macrolide inactivation gene cluster mphA-mrx-mphR. The other one was an integron-free SGI1 and contained a 183-bp truncated S025 next to IS6100 and S044. PMID:25918148

  2. Molecular Characteristics of Salmonella Genomic Island 1 in Proteus mirabilis Isolates from Poultry Farms in China

    PubMed Central

    Lei, Chang-Wei; Zhang, An-Yun; Liu, Bi-Hui; Guan, Zhong-Bin; Xu, Chang-Wen; Xia, Qing-Qing; Cheng, Han; Zhang, Dong-Dong

    2014-01-01

    Six out of the 64 studied Proteus mirabilis isolates from 11 poultry farms in China contained Salmonella genomic island 1 (SGI1). PCR mapping showed that the complete nucleotide sequences of SGI1s ranged from 33.2 to 42.5 kb. Three novel variants, SGI1-W, SGI1-X, and SGI1-Y, have been characterized. Resistance genes lnuF, dfrA25, and qnrB2 were identified in SGI1 for the first time. PMID:25267683

  3. Molecular characteristics of Salmonella genomic island 1 in Proteus mirabilis isolates from poultry farms in China.

    PubMed

    Lei, Chang-Wei; Zhang, An-Yun; Liu, Bi-Hui; Wang, Hong-Ning; Guan, Zhong-Bin; Xu, Chang-Wen; Xia, Qing-Qing; Cheng, Han; Zhang, Dong-Dong

    2014-12-01

    Six out of the 64 studied Proteus mirabilis isolates from 11 poultry farms in China contained Salmonella genomic island 1 (SGI1). PCR mapping showed that the complete nucleotide sequences of SGI1s ranged from 33.2 to 42.5 kb. Three novel variants, SGI1-W, SGI1-X, and SGI1-Y, have been characterized. Resistance genes lnuF, dfrA25, and qnrB2 were identified in SGI1 for the first time. PMID:25267683

  4. First report of an OXA-48-producing multidrug-resistant Proteus mirabilis strain from Gaza, Palestine.

    PubMed

    Chen, Liang; Al Laham, Nahed; Chavda, Kalyan D; Mediavilla, Jose R; Jacobs, Michael R; Bonomo, Robert A; Kreiswirth, Barry N

    2015-07-01

    We report the first multidrug-resistant Proteus mirabilis strain producing the carbapenemase OXA-48 (Pm-OXA-48) isolated at Al-Shifa hospital in Gaza, Palestine. Draft genome sequencing of Pm-OXA-48 identified 16 antimicrobial resistance genes, encoding resistance to ?-lactams, aminoglycosides, fluoroquinolones, phenicols, streptothricin, tetracycline, and trimethoprim-sulfamethoxazole. Complete sequencing of the bla(OXA-48)-harboring plasmid revealed that it is a 72 kb long IncL/M plasmid, harboring carbapenemase gene bla(OXA-48), extended spectrum ?-lactamase gene bla(CTX-M-14), and aminoglycoside resistance genes strA, strB, and aph(3')-VIb. PMID:25896692

  5. Interferences in the Optimization of the MTT Assay for Viability Estimation of Proteus mirabilis

    PubMed Central

    Grela, Ewa; Z?bek, Adam; Grabowiecka, Agnieszka

    2015-01-01

    Background: The chromogenic assay based on MTT bioreduction was adapted to Proteus mirabilis viability estimations. We primarily intended to use the assay for the evaluation of novel antimicrobial compounds, including structures with possible permeabilizing activity. Therefore, the influence of basic permeabilizing agents like Triton X-100 and EDTA upon the MTT assay was studied. Methods: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) was used as a substrate for the whole-cell dehydrogenase activity estimations. The amount of formazan product was evaluated in the end-point reactions terminated with acidic isopropanol or in the continuous reactions run in the presence of low detergent concentrations. Results: The generally established procedure of the end product dissolution with acidic isopropanol caused absorbance instability which strongly affected the results accuracy. The disadvantage was especially pronounced when the assay was conducted in Mueller-Hinton Broth. PBS with 0.01% Triton X-100 used as the reaction medium allowed to omit the formazan dissolution step and follow the microbial MTT reduction in a continuous mode. It was observed that in Proteus mirabilis with a compromised outer membrane the assay score was artificially increased above the untreated control. Conclusion: The dependence of the assay results on the cell integrity might be a major drawback of the MTT assay application for the evaluation of novel antimicrobials against Gram-negative microorganisms. On the other hand, the MTT reduction could be conveniently used to assay the permeabilization degree in biotechnological protocols.

  6. Modified insulator semiconductor electrode with functionalized nanoparticles for Proteus mirabilis bacteria biosensor development.

    PubMed

    Braham, Yosra; Barhoumi, Houcine; Maaref, Abderrazak; Bakhrouf, Amina; Jaffrezic-Renault, Nicole

    2013-12-01

    The development of enzymatic sensors for biological purposes such as biomedicine, pharmacy, food industry, and environmental toxicity requires the purification step of the enzyme. To prevent the loss of the enzyme activity, a new strategy is held in order to immobilize the bacteria. It will constitute the biological sensing element leading to a high operational stability and multiple adaptations to various conditions such as temperature, pH and ionic strength changes. In this work we describe the development of a urea biosensor by immobilizing Proteus mirabilis bacteria onto an insulator-semiconductor electrode on functionalized Fe3O4 nanoparticles (NPs), using cationic, Poly (allylamine hydrochloride) then anionic, Poly (sodium 4-styrenesulfonate) polyelectrolytes, BSA (serum bovin albumin), and glutaraldehyde as a cross-linking agent. The response of P. mirabilis to urea addition is evaluated in homogeneous and heterogeneous phases. Before the immobilization step, the activity of urease produced from the P. mirabilis bacteria was attempted using the ion ammonium selective electrodes (ISEs). Adhesion of the bacteria cells on IS electrodes have been studied using contact angle measurements. After immobilization of the bacteria, on the (Si/SiO2/Si3N4) and (Si/SiO2) substrates, the relationship between the evolution of the flat band potential ?VFB and the urea concentration is found to be linear for values ranging from 10(-2)M to 10(-5)M. PMID:24094152

  7. Novel Insights into the Proteus mirabilis Crystalline Biofilm Using Real-Time Imaging

    PubMed Central

    Wilks, Sandra A.; Fader, Mandy J.; Keevil, C. William

    2015-01-01

    The long-term use of indwelling catheters results in a high risk from urinary tract infections (UTI) and blockage. Blockages often occur from crystalline deposits, formed as the pH rises due to the action of urease-producing bacteria; the most commonly found species being Proteus mirabilis. These crystalline biofilms have been found to develop on all catheter materials with P. mirabilis attaching to all surfaces and forming encrustations. Previous studies have mainly relied on electron microscopy to describe this process but there remains a lack of understanding into the stages of biofilm formation. Using an advanced light microscopy technique, episcopic differential interference contrast (EDIC) microscopy combined with epifluorescence (EF), we describe a non-destructive, non-contact, real-time imaging method used to track all stages of biofilm development from initial single cell attachment to complex crystalline biofilm formation. Using a simple six-well plate system, attachment of P. mirabilis (in artificial urine) to sections of silicone and hydrogel latex catheters was tracked over time (up to 24 days). Using EDIC and EF we show how initial attachment occurred in less than 1 h following exposure to P. mirabilis. This was rapidly followed by an accumulation of an additional material (indicated to be carbohydrate based using lectin staining) and the presence of highly elongated, motile cells. After 24 h exposure, a layer developed above this conditioning film and within 4 days the entire surface (of both catheter materials) was covered with diffuse crystalline deposits with defined crystals embedded. Using three-dimensional image reconstruction software, cells of P. mirabilis were seen covering the crystal surfaces. EDIC microscopy could resolve these four components of the complex crystalline biofilm and the close relationship between P. mirabilis and the crystals. This real-time imaging technique permits study of this complex biofilm development with no risk of artefacts due to sample manipulation. A full understanding of the stages and components involved in crystalline encrustation formation will aid in the development of new protocols to manage and ultimately prevent catheter blockage. PMID:26516766

  8. Activity of Proteus mirabilis FliL Is Viscosity Dependent and Requires Extragenic DNA

    PubMed Central

    Lee, Yi-Ying; Patellis, Julius

    2013-01-01

    Proteus mirabilis is a urinary tract pathogen and well known for its ability to move over agar surfaces by flagellum-dependent swarming motility. When P. mirabilis encounters a highly viscous environment, e.g., an agar surface, it differentiates from short rods with few flagella to elongated, highly flagellated cells that lack septa and contain multiple nucleoids. The bacteria detect a surface by monitoring the rotation of their flagellar motors. This process involves an enigmatic flagellar protein called FliL, the first gene in an operon (fliLMNOPQR) that encodes proteins of the flagellar rotor switch complex and flagellar export apparatus. We used a fliL knockout mutant to gain further insight into the function of FliL. Loss of FliL results in cells that cannot swarm (Swr?) but do swim (Swm+) and produces cells that look like wild-type swarmer cells, termed “pseudoswarmer cells,” that are elongated, contain multiple nucleoids, and lack septa. Unlike swarmer cells, pseudoswarmer cells are not hyperflagellated due to reduced expression of flaA (the gene encoding flagellin), despite an increased transcription of both flhD and fliA, two positive regulators of flagellar gene expression. We found that defects in fliL prevent viscosity-dependent sensing of a surface and viscosity-dependent induction of flaA transcription. Studies with fliL cells unexpectedly revealed that the fliL promoter, fliL coding region, and a portion of fliM DNA are needed to complement the Swr? phenotype. The data support a dual role for FliL as a critical link in sensing a surface and in the maintenance of flagellar rod integrity. PMID:23222728

  9. Production of a High Efficiency Microbial Flocculant by Proteus mirabilis TJ-1 Using Compound Organic Wastewater

    NASA Astrophysics Data System (ADS)

    Zhang, Zhiqiang; Xia, Siqing; Zhang, Jiao

    2010-11-01

    The production of a high efficiency microbial flocculant (MBF) by Proteus mirabilis TJ-1 using compound organic wastewater was investigated. To cut down the cost of the MBF production, several nutritive organic wastewaters were selected to replace glucose and peptone as the carbon source and the nitrogen source in the optimized medium of strain TJ-1, respectively. The compound wastewater of the milk candy and the soybean milk was found to be good carbon source and nitrogen source for this strain to produce MBF. The cost-effective culture medium consists of (per liter): 800 mL wastewater of milk candy, 200 mL wastewater of soybean milk, 0.3 g MgSO4?7 H2O, 5 g K2HPO4, 2 g and KH2PO4, pH 7.0. The economic cost for the MBF production can be cut down over a half by using the developed culture medium. Furthermore, the utilization of the two wastewaters in the preparation of culture medium of strain TJ-1 can not only save their big treatment cost, but also realize their resource reuse.

  10. Complicated Catheter-Associated Urinary Tract Infections Due to Escherichia coli and Proteus mirabilis

    PubMed Central

    Jacobsen, S. M.; Stickler, D. J.; Mobley, H. L. T.; Shirtliff, M. E.

    2008-01-01

    Catheter-associated urinary tract infections (CAUTIs) represent the most common type of nosocomial infection and are a major health concern due to the complications and frequent recurrence. These infections are often caused by Escherichia coli and Proteus mirabilis. Gram-negative bacterial species that cause CAUTIs express a number of virulence factors associated with adhesion, motility, biofilm formation, immunoavoidance, and nutrient acquisition as well as factors that cause damage to the host. These infections can be reduced by limiting catheter usage and ensuring that health care professionals correctly use closed-system Foley catheters. A number of novel approaches such as condom and suprapubic catheters, intermittent catheterization, new surfaces, catheters with antimicrobial agents, and probiotics have thus far met with limited success. While the diagnosis of symptomatic versus asymptomatic CAUTIs may be a contentious issue, it is generally agreed that once a catheterized patient is believed to have a symptomatic urinary tract infection, the catheter is removed if possible due to the high rate of relapse. Research focusing on the pathogenesis of CAUTIs will lead to a better understanding of the disease process and will subsequently lead to the development of new diagnosis, prevention, and treatment options. PMID:18202436

  11. Role of RppA in the Regulation of Polymyxin B Susceptibility, Swarming, and Virulence Factor Expression in Proteus mirabilis?

    PubMed Central

    Wang, Won-Bo; Chen, I-Chun; Jiang, Sin-Sien; Chen, Hui-Ru; Hsu, Chia-Yu; Hsueh, Po-Ren; Hsu, Wei-Bin; Liaw, Shwu-Jen

    2008-01-01

    Proteus mirabilis, a human pathogen that frequently causes urinary tract infections, is intrinsically highly resistant to cationic antimicrobial peptides, such as polymyxin B (PB). To explore the mechanisms underlying P. mirabilis resistance to PB, a mutant which displayed increased (>160-fold) sensitivity to PB was identified by transposon mutagenesis. This mutant was found to have Tn5 inserted into a novel gene, rppA. Sequence analysis indicated that rppA may encode a response regulator of the two-component system and is located upstream of the rppB gene, which may encode a membrane sensor kinase. An rppA knockout mutant of P. mirabilis had an altered lipopolysaccharide (LPS) profile. The LPS purified from the rppA knockout mutant could bind more PB than the LPS purified from the wild type. These properties of the rppA knockout mutant may contribute to its PB-sensitive phenotype. The rppA knockout mutant exhibited greater swarming motility and cytotoxic activity and expressed higher levels of flagellin and hemolysin than the wild type, suggesting that RppA negatively regulates swarming, hemolysin expression, and cytotoxic activity in P. mirabilis. PB could modulate LPS synthesis and modification, swarming, hemolysin expression, and cytotoxic activity in P. mirabilis through an RppA-dependent pathway, suggesting that PB could serve as a signal to regulate RppA activity. Finally, we demonstrated that the expression of rppA was up-regulated by a low concentration of PB and down-regulated by a high concentration of Mg2+. Together, these data highlight the essential role of RppA in regulating PB susceptibility and virulence functions in P. mirabilis. PMID:18316383

  12. Perturbation of FliL Interferes with Proteus mirabilis Swarmer Cell Gene Expression and Differentiation

    PubMed Central

    Cusick, Kathleen; Lee, Yi-Ying; Youchak, Brian

    2012-01-01

    Proteus mirabilis is a dimorphic, motile bacterium often associated with urinary tract infections. Colonization of urinary tract surfaces is aided by swarmer cell differentiation, which is initiated by inhibition of flagellar rotation when the bacteria first contact a surface. Mutations in fliL, encoding a flagellar structural protein with an enigmatic function, result in the inappropriate production of differentiated swarmer cells, called pseudoswarmer cells, under noninducing conditions, indicating involvement of FliL in the surface sensing pathway. In the present study, we compared the fliL transcriptome with that of wild-type swarmer cells and showed that nearly all genes associated with motility (flagellar class II and III genes) and chemotaxis are repressed. In contrast, spontaneous motile revertants of fliL cells that regained motility yet produced differentiated swarmer cells under noninducing conditions transcribed flagellar class II promoters at consistent levels. Expression of umoA (a known regulator of swarmer cells), flgF, and flgI increased significantly in both swarmer and pseudoswarmer cells, as did genes in a degenerate prophage region situated immediately adjacent to the Rcs phosphorelay system. Unlike swarmer cells, pseudoswarmers displayed increased activity, rather than transcription, of the flagellar master regulatory protein, FlhD4C2, and analyses of the fliL parent strain and its motile revertants showed that they result from mutations altering the C-terminal 14 amino acids of FliL. Collectively, the data suggest a functional role for the C terminus of FliL in surface sensing and implicate UmoA as part of the signal relay leading to the master flagellar regulator FlhD4C2, which ultimately controls swarmer cell differentiation. PMID:22081397

  13. Sequential unfolding of the hemolysin two-partner secretion domain from Proteus mirabilis.

    PubMed

    Wimmer, Megan R; Woods, Christopher N; Adamczak, Kyle J; Glasgow, Evan M; Novak, Walter R P; Grilley, Daniel P; Weaver, Todd M

    2015-11-01

    Protein secretion is a major contributor to Gram-negative bacterial virulence. Type Vb or two-partner secretion (TPS) pathways utilize a membrane bound ?-barrel B component (TpsB) to translocate large and predominantly virulent exoproteins (TpsA) through a nucleotide independent mechanism. We focused our studies on a truncated TpsA member termed hemolysin A (HpmA265), a structurally and functionally characterized TPS domain from Proteus mirabilis. Contrary to the expectation that the TPS domain of HpmA265 would denature in a single cooperative transition, we found that the unfolding follows a sequential model with three distinct transitions linking four states. The solvent inaccessible core of HpmA265 can be divided into two different regions. The C-proximal region contains nonpolar residues and forms a prototypical hydrophobic core as found in globular proteins. The N-proximal region of the solvent inaccessible core, however, contains polar residues. To understand the contributions of the hydrophobic and polar interiors to overall TPS domain stability, we conducted unfolding studies on HpmA265 and site-specific mutants of HpmA265. By correlating the effect of individual site-specific mutations with the sequential unfolding results we were able to divide the HpmA265 TPS domain into polar core, nonpolar core, and C-terminal subdomains. Moreover, the unfolding studies provide quantitative evidence that the folding free energy for the polar core subdomain is more favorable than for the nonpolar core and C-terminal subdomains. This study implicates the hydrogen bonds shared among these conserved internal residues as a primary means for stabilizing the N-proximal polar core subdomain. PMID:26350294

  14. Modulation of pulmonary defense mechanisms by acute exposures to nitrogen dioxide. [Staphylococcus aureus; Proteus mirabilis; Pasteurella pneumotropica

    SciTech Connect

    Jakab, G.J.

    1987-02-01

    The effect of acute exposures to NO/sub 2/ on the antibacterial defenses of the murine lung was assessed following inhalation challenges with Staphylococcus aureus, Proteus mirabilis, and Pasteurella pneumotropica. With S. aureus pulmonary antibacterial defenses were suppressed at NO/sub 2/ levels of 4.0 ppm and greater. Exposure to 10.0 ppm enhanced the intrapulmonary killing of P. mirabilis which correlated with an increase in the phagocytic cell populations lavaged from the lungs; at 20.0 ppm bactericidal activity against P. mirabilis was impaired. Pulmonary antibacterial defenses against P. pneumotropica were impaired at 10.0 ppm which correlated with a decrease in the retrieved phagocytic lung cell population. Reversing the order of treatment (ie., NO/sub 2/ exposure prior to bacterial challenge) raised the threshold concentration for NO/sub 2/-induced impairment of intrapulmonary bacterial killing. With S. aureus the effect was not observed at 5.0 ppm but at 10.0 ppm and with P. mirabilis not at 20.0 ppm but at 30.0 ppm intrapulmonary killing was enhanced. Exposures up to 20.0 ppm of NO/sub 2/ did not effect the physical translocation mechanisms of the lung as quantitated by declines in pulmonary radiotracer activity following aerogenic challenge with /sup 32/P-labeled staphylococci.

  15. Emergence of Extensively Drug-Resistant Proteus mirabilis Harboring a Conjugative NDM-1 Plasmid and a Novel Salmonella Genomic Island 1 Variant, SGI1-Z.

    PubMed

    Qin, Shangshang; Qi, Hui; Zhang, Qijing; Zhao, Di; Liu, Zhen-Zhen; Tian, Hao; Xu, Lijuan; Xu, Hui; Zhou, Mengmeng; Feng, Xianju; Liu, Hong-Min

    2015-10-01

    Acquisition of blaNDM-1 in bacterial species, such as Proteus mirabilis that is intrinsically resistant to tetracycline, tigecycline and colistin, will make clinical treatment extremely difficult. Here, we characterized an NDM-1-producing clinical isolate of P. mirabilis (PM58) that displayed an extensively drug-resistant (XDR) phenotype, susceptible only to aztreonam. Molecular analysis revealed that PM58 harbored both a conjugative NDM-1 plasmid and a novel Salmonella genomic island 1 variant on chromosome. PMID:26195511

  16. Various intensity of Proteus mirabilis-induced crystallization resulting from the changes in the mineral composition of urine.

    PubMed

    Torzewska, Agnieszka; Ró?alski, Antoni

    2015-01-01

    Infectious urolithiasis is a result of recurrent and chronic urinary tract infections caused by urease-positive bacteria, especially Proteus mirabilis. The main role in the development of this kind of stones is played by bacterial factors such as urease and extracellular polysaccharides, but urinary tract environment also contributes to this process. We used an in vitro model to establish how the changes in the basic minerals concentrations affect the intensity of crystallization which occurs in urine. In each experiment crystallization was induced by an addition of P. mirabilis to artificial urine with a precisely defined chemical composition. Crystallization intensity was determined using the spectrophotometric microdilution method and the chemical composition of formed crystals was established by atomic absorption spectroscopy and colorimetric methods. Increasing the concentration of all crystals forming ions such as Mg(2+), Ca(2+) and phosphate strongly intensified the process of crystallization, whereas reducing the amount of these components below the proper physiological concentration did not affect its intensity. The inhibitory influence of citrate on calcium and magnesium phosphate crystallization and competitive actions of calcium and oxalate ions on struvite crystals formation were not confirmed. In the case of infectious stones the chemical composition of urine plays an important role, which creates a necessity to support the treatment by developing a model of proper diet. PMID:25654361

  17. Arginine promotes Proteus mirabilis motility and fitness by contributing to conservation of the proton gradient and proton motive force

    PubMed Central

    Armbruster, Chelsie E; Hodges, Steven A; Smith, Sara N; Alteri, Christopher J; Mobley, Harry L T

    2014-01-01

    Swarming contributes to Proteus mirabilis pathogenicity by facilitating access to the catheterized urinary tract. We previously demonstrated that 0.1–20 mmol/L arginine promotes swarming on normally nonpermissive media and that putrescine biosynthesis is required for arginine-induced swarming. We also previously determined that arginine-induced swarming is pH dependent, indicating that the external proton concentration is critical for arginine-dependent effects on swarming. In this study, we utilized survival at pH 5 and motility as surrogates for measuring changes in the proton gradient (?pH) and proton motive force (?H+) in response to arginine. We determined that arginine primarily contributes to ?pH (and therefore ?H+) through the action of arginine decarboxylase (speA), independent of the role of this enzyme in putrescine biosynthesis. In addition to being required for motility, speA also contributed to fitness during infection. In conclusion, consumption of intracellular protons via arginine decarboxylase is one mechanism used by P. mirabilis to conserve ?pH and ?H+ for motility. PMID:25100003

  18. Differential Association of F? Plasmid and R Plasmid Deoxyribonucleic Acid with a Rapidly Sedimenting Fraction of a Proteus mirabilis Lysate

    PubMed Central

    Taichman, Lorne; Rownd, Robert H.

    1977-01-01

    We have examined the association of an F? plasmid and an R plasmid in Proteus mirabilis with a rapidly sedimenting material that is generated by sodium dodecyl sulfate lysis and low speed centrifugation. Virtually all of the chromosomal deoxyribonucleic acid (DNA) and the F? plasmid DNA are associated with the rapidly sedimenting material after gentle lysis and centrifugation. A portion of R plasmid NR1 DNA (usually 5 to 25%) is not bound to the rapidly sedimenting material and is recovered in the supernatant fraction. This difference in binding is not related to the size of the plasmid DNA, since F? plasmids and R plasmids of different molecular weights showed the same behavior. R plasmid DNA labeled by a brief pulse of [3H]thymine is recovered in the supernatant fraction to a lower extent than the total R plasmid DNA. It would appear that R plasmid replication takes place in association with the rapidly sedimenting material. With prolongation of the [3H]thymine pulse, the [3H]thymine-labeled R plasmid DNA is recovered in the supernatant fraction with the same probability as the total R plasmid DNA. This finding indicates that a change in R plasmid attachment to the rapidly sedimenting material occurs some time after its replication. The differences observed in the replication of F? plasmids and R plasmids in P. mirabilis may be related to their different modes of association with the rapidly sedimenting material. PMID:324981

  19. Evolution and Spread of a Multidrug-Resistant Proteus mirabilis Clone with Chromosomal AmpC-Type Cephalosporinases in Europe?

    PubMed Central

    D'Andrea, M. M.; Literacka, E.; Zioga, A.; Giani, T.; Baraniak, A.; Fiett, J.; Sadowy, E.; Tassios, P. T.; Rossolini, G. M.; Gniadkowski, M.; Miriagou, V.

    2011-01-01

    Proteus mirabilis isolates obtained in 1999 to 2008 from three European countries were analyzed; all carried chromosomal AmpC-type cephalosporinase blaCMY genes from a Citrobacter freundii origin (blaCMY-2-like genes). Isolates from Poland harbored several blaCMY genes (blaCMY-4, blaCMY-12, blaCMY-14, blaCMY-15, and blaCMY-38 and the new gene blaCMY-45), while isolates from Italy and Greece harbored blaCMY-16 only. Earlier isolates with blaCMY-4 or blaCMY-12, recovered in France from Greek and Algerian patients, were also studied. All isolates showed striking similarities. Their blaCMY genes resided within ISEcp1 transposition modules, named Tn6093, characterized by a 110-bp distance between ISEcp1 and blaCMY, and identical fragments of both C. freundii DNA and a ColE1-type plasmid backbone. Moreover, these modules were inserted into the same chromosomal site, within the pepQ gene. Since ColE1 plasmids carrying ISEcp1 with similar C. freundii DNA fragments (Tn6114) had been identified earlier, it is likely that a similar molecule had mediated at some stage this DNA transfer between C. freundii and P. mirabilis. In addition, isolates with blaCMY-12, blaCMY-15, and blaCMY-38 genes harbored a second blaCMY copy within a shorter ISEcp1 module (Tn6113), always inserted downstream of the ppiD gene. Sequence analysis of all mobile blaCMY-2-like genes indicated that those integrated in the P. mirabilis chromosome form a distinct cluster that may have evolved by the stepwise accumulation of mutations. All of these observations, coupled to strain typing data, suggest that the blaCMY genes studied here may have originated from a single ISEcp1-mediated mobilization-transfer-integration process, followed by the spread and evolution of a P. mirabilis clone over time and a large geographic area. PMID:21402851

  20. Visualization of Proteus mirabilis morphotypes in the urinary tract: the elongated swarmer cell is rarely observed in ascending urinary tract infection.

    PubMed

    Jansen, Angela M; Lockatell, C Virginia; Johnson, David E; Mobley, Harry L T

    2003-06-01

    Proteus mirabilis, a common cause of nosocomial and catheter-associated urinary tract infection, colonizes the bladder and ascends the ureters to the proximal tubules of the kidneys, leading to the development of acute pyelonephritis. P. mirabilis is capable of swarming, a form of multicellular behavior in which bacteria differentiate from the short rod typical of members of the family Enterobacteriaceae, termed the swimmer cell, into hyperflagellated elongated bacteria capable of rapid and coordinated population migration across surfaces, called the swarmer cell. There has been considerable debate as to which morphotype predominates during urinary tract infection. P. mirabilis(pBAC001), which expresses green fluorescent protein in both swimming and swarming morphotypes, was constructed to quantify the prevalence of each morphotype in ascending urinary tract infection. Transurethral inoculation of P. mirabilis(pBAC001) resulted in ascending urinary tract infection and kidney pathology in mice examined at both 2 and 4 days postinoculation. Using confocal microscopy, we were able to investigate the morphotypes of the bacteria in the urinary tract. Of 5,087 bacteria measured in bladders, ureters, and kidneys, only 7 (0.14%) were identified as swarmers. MR/P fimbria expression, which correlates with the swimmer phenotype, is prevalent on bacteria in the ureters and bladder. We conclude that, by far, the predominant morphotype present in the urinary tract during ascending infection is the short rod-the swimmer cell. PMID:12761147

  1. The Novel CTX-M-116 ?-Lactamase Gene Discovered in Proteus mirabilis Is Composed of Parts of the CTX-M-22 and CTX-M-23 Genes

    PubMed Central

    Pryamchuk, S.; Kruglov, A.; Abaev, I.; Pecherskikh, E.; Kartsev, N.; Svetoch, E.; Dyatlov, I.

    2013-01-01

    The novel ?-lactamase gene blaCTX-M-116 was identified in a Proteus mirabilis nosocomial isolate recovered from the urine of a patient in Moscow in 2005. DNA sequence analysis showed blaCTX-M-116 to be a hybrid gene consisting of 5? blaCTX-M-23 (nucleotides 1 to 278) and 3? blaCTX-M-22 (nucleotides 286 to 876) moieties separated by an intervening putative site of recombination (GTTAAAT). A retrospective analysis of available blaCTX-M genes in the GenBank database revealed 19 blaCTX-M genes that display the same hybrid structure. PMID:23318795

  2. Phenotypic and Molecular Characterization of Plasmid Mediated AmpC ?-Lactamases among Escherichia coli, Klebsiella spp., and Proteus mirabilis Isolated from Urinary Tract Infections in Egyptian Hospitals

    PubMed Central

    Helmy, Mai M.; Wasfi, Reham

    2014-01-01

    The incidence of resistance by Enterobacteriaceae to ?-lactam/?-lactamase inhibitors combination is increasing in Egypt. Three phenotypic techniques, comprising AmpC disk diffusion and inhibition dependent methods using phenylboronic acid (PBA) and cloxacillin, were compared to PCR based method for detection of plasmid mediated AmpC ?-lactamase in common urinary tract isolates. A total of 143 isolates, including E. coli, Klebsiella pneumonia, and Proteus mirabilis, were collected from urinary tract infections cases in Egyptian hospitals. Plasmid encoded AmpC genes were detected by PCR in 88.46% of cefoxitin resistant isolates. The most prevalent AmpC gene family was CIT including CMY-2, CMY-4, and two CMY-2 variants. The second prevalent gene was DHA-1 which was detected in E. coli and Klebsiella pneumonia. The genes EBC, FOX, and MOX were also detected but in small percentage. Some isolates were identified as having more than one pAmpC gene. The overall sensitivity and specificity of phenotypic tests for detection of AmpC ?-lactamase showed that AmpC disk diffusion and inhibition dependent method by cloxacillin were the most sensitive and the most specific disk tests. PCR remains the gold standard for detection of AmpC ?-lactamases. This study represents the first report of CMY-2 variants of CMY-42 and CMY-102 ?-lactamase-producing E. coli, Klebsiella pneumonia, and Proteus mirabilis isolates in Egypt. PMID:25003107

  3. Comparative in vitro studies on disodium EDTA effect with and without Proteus mirabilis on the crystallization of carbonate apatite and struvite

    NASA Astrophysics Data System (ADS)

    Prywer, Jolanta; Olszynski, Marcin; Torzewska, Agnieszka; Mielniczek-Brzóska, Ewa

    2014-06-01

    Effect of disodium EDTA (salt of ethylenediamine tetraacetic acid) on the crystallization of struvite and carbonate apatite was studied. To evaluate such an effect we performed an experiment of struvite and carbonate apatite growth from artificial urine. The crystallization process was induced by Proteus mirabilis to mimic the real urinary tract infection, which usually leads to urinary stone formation. The results demonstrate that disodium EDTA exhibits the effect against P. mirabilis retarding the activity of urease - an enzyme produced by these microorganisms. The spectrophotometric results demonstrate that, with and without P. mirabilis, the addition of disodium EDTA increases the induction time and decreases the growth efficiency compared to the baseline (without disodium EDTA). These results are discussed from the standpoint of speciation of complexes formed in the solution of artificial urine in the presence of disodium EDTA. The size of struvite crystals was found to decrease in the presence of disodium EDTA. However, struvite crystals are larger in the presence of bacteria while the crystal morphology and habit remain unchanged.

  4. In silico design of fusion protein of FimH from uropathogenic Escherichia coli and MrpH from Proteus mirabilis against urinary tract infections

    PubMed Central

    Habibi, Mehri; Asadi Karam, Mohammad Reza; Bouzari, Saeid

    2015-01-01

    Background: Urinary tract infections (UTIs) caused by uropathogenic Escherichia coli (UPEC) and Proteus mirabilis are the most important pathogens causing UTIs. The FimH from type 1 pili of UPEC and the MrpH from P. mirabilis play critical roles in the UTI process and have presented as ideal vaccine candidates against UTIs. There is no effective vaccine against UTI and the development of an ideal UTI vaccine is required. Materials and Methods: In this study, we planned to design a novel fusion protein of FimH from UPEC and MrpH from P. mirabilis. For this purpose, we modeled fusion protein forms computationally using the Iterative Threading Assembly Refinement (I-TASSER) server and evaluated their interactions with toll-like receptor 4 (TLR4). The best fusion protein was constructed using overlap extension polymerase chain reaction (OE-PCR) and the biological activity of fusion was evaluated by the induction of interleukin-8 (IL-8) in the HT-29 cell line. Results: Our study indicated that based on the Protein Structure Analysis (ProSA)-web and the docking results, MrpH.FimH showed better results than did FimH.MrpH, and it was selected for construction. The results of bioassay on the HT-29 showed that FimH and MrpH.FimH induced significantly higher IL-8 responses than untreated cells or MrpH alone in the cell line tested. Conclusions: In the present study, we designed and constructed the novel fusion protein MrpH.FimH from UPEC and P. mirabilis based on in silico methods. Our bioassay results indicate that the MrpH.FimH fusion protein is active and capable of inducing immune responses. PMID:26605246

  5. Comparison of quantitative and qualitative antibody-producing cell responses to lipopolysaccharide in cell walls of the bacterial form and in membranes of the protoplast L-form of Proteus mirabilis.

    PubMed Central

    Karch, H; Nixdorff, K

    1980-01-01

    Membranes of the stable protoplast L-form of Proteus mirabilis strain VI were highly immunogenic carriers of lipopolysaccharide when compared with the immune responses to lipopolysaccharide contained in cell walls of the bacterial form of this organism. Images Fig. 1 PMID:7002796

  6. Initiation of Swarming Motility by Proteus mirabilis Occurs in Response to Specific Cues Present in Urine and Requires Excess l-Glutamine

    PubMed Central

    Armbruster, Chelsie E.; Hodges, Steven A.

    2013-01-01

    Proteus mirabilis, a leading cause of catheter-associated urinary tract infection (CaUTI), differentiates into swarm cells that migrate across catheter surfaces and medium solidified with 1.5% agar. While many genes and nutrient requirements involved in the swarming process have been identified, few studies have addressed the signals that promote initiation of swarming following initial contact with a surface. In this study, we show that P. mirabilis CaUTI isolates initiate swarming in response to specific nutrients and environmental cues. Thirty-three compounds, including amino acids, polyamines, fatty acids, and tricarboxylic acid (TCA) cycle intermediates, were tested for the ability to promote swarming when added to normally nonpermissive media. l-Arginine, l-glutamine, dl-histidine, malate, and dl-ornithine promoted swarming on several types of media without enhancing swimming motility or growth rate. Testing of isogenic mutants revealed that swarming in response to the cues required putrescine biosynthesis and pathways involved in amino acid metabolism. Furthermore, excess glutamine was found to be a strict requirement for swarming on normal swarm agar in addition to being a swarming cue under normally nonpermissive conditions. We thus conclude that initiation of swarming occurs in response to specific cues and that manipulating concentrations of key nutrient cues can signal whether or not a particular environment is permissive for swarming. PMID:23316040

  7. Alteration of the immunoglobulin G subclass responses in mice to lipopolysaccharide: effects of nonbacterial proteins and bacterial membrane phospholipids or outer membrane proteins of Proteus mirabilis.

    PubMed Central

    Karch, H; Gmeiner, J; Nixdorff, K

    1983-01-01

    The immunoglobulin M (IgM) and the IgG1, IgG2ab, and IgG3 subclasses of plaque-forming cells (PFC) specific for lipopolysaccharide (LPS) were measured after immunization of mice with LPS alone and compared with the responses to LPS in combination with nonbacterial proteins and with bacterial membrane phospholipid vesicles or two major outer membrane proteins from Proteus mirabilis. The relative numbers of IgG PFC belonging to the IgG1, IgG2, or IgG3 subclasses induced by immunization with LPS alone depended upon the type of LPS administered. Phospholipids and the proteins effected characteristic alterations in not only the strength but also the subclass of the IgG responses to LPS. The results suggest that the hydrophobic-hydrophilic nature or state of aggregation of the preparations plays a role in the induction of IgG1 and IgG2 subclasses of PFC specific for LPS. Complex formation with LPS and adjuvant was apparently necessary to obtain these effects. Images PMID:6187689

  8. Chromosome-Encoded AmpC and CTX-M Extended-Spectrum ?-Lactamases in Clinical Isolates of Proteus mirabilis from Korea?

    PubMed Central

    Song, Wonkeun; Kim, Juwon; Bae, Il Kwon; Jeong, Seok Hoon; Seo, Young Hee; Shin, Jong Hee; Jang, Sook Jin; Uh, Young; Shin, Jeong Hwan; Lee, Mi-Kyung; Lee, Kyungwon

    2011-01-01

    Among 222 Proteus mirabilis clinical isolates collected from 17 hospitals in Korea in 2008, 28 (12.6%) and 8 (3.6%) isolates exhibited extended-spectrum ?-lactamase (ESBL) and AmpC phenotypes, respectively. The most common type of ESBL gene identified by PCR and sequencing experiments was blaCTX-M-14a (n = 12). The blaCTX-M-90 (n = 4), blaCTX-M-15 (n = 3), blaCTX-M-12 (n = 3), blaCTX-M-2 (n = 2), blaCTX-M-14b (n = 1), blaTEM-52 (n = 5), and blaSHV-12 (n = 1) genes were also detected. Eight isolates carried an AmpC ?-lactamase gene, such as blaCMY-2 (n = 6) or blaDHA-1 (n = 2). All bla genes encoding CTX-M-1- and CTX-M-9-type enzymes and all blaCMY-2 genes were preceded by ISEcp1-like elements. The blaCTX-M-2 gene found in two isolates was located on a complex class 1 integron. The blaDHA-1 gene was preceded by a transcriptional regulator gene and was followed by phage shock protein genes. The blaCTX-M genes were located on the chromosome in 21 isolates. A plasmid location for the blaCTX-M gene was found in only four isolates: the blaCTX-M-14a gene was located on ?150-kbp IncA/C plasmids in three isolates and on a ?50-kbp IncN plasmid in one isolate. The blaTEM-52 gene was located on ?50-kbp IncN plasmids in all five isolates. The AmpC ?-lactamase genes were located on the chromosome in seven of eight isolates; one isolate carried the blaCMY-2 gene on a ?150-kbp IncA/C plasmid. Our results show that a chromosomal location of CTX-M ESBL and AmpC ?-lactamase genes in P. mirabilis is no longer an unusual phenomenon in hospital environments. PMID:21282448

  9. Crystal Structure of the Dithiol Oxidase DsbA Enzyme from Proteus Mirabilis Bound Non-covalently to an Active Site Peptide Ligand

    PubMed Central

    Kurth, Fabian; Duprez, Wilko; Premkumar, Lakshmanane; Schembri, Mark A.; Fairlie, David P.; Martin, Jennifer L.

    2014-01-01

    The disulfide bond forming DsbA enzymes and their DsbB interaction partners are attractive targets for development of antivirulence drugs because both are essential for virulence factor assembly in Gram-negative pathogens. Here we characterize PmDsbA from Proteus mirabilis, a bacterial pathogen increasingly associated with multidrug resistance. PmDsbA exhibits the characteristic properties of a DsbA, including an oxidizing potential, destabilizing disulfide, acidic active site cysteine, and dithiol oxidase catalytic activity. We evaluated a peptide, PWATCDS, derived from the partner protein DsbB and showed by thermal shift and isothermal titration calorimetry that it binds to PmDsbA. The crystal structures of PmDsbA, and the active site variant PmDsbAC30S were determined to high resolution. Analysis of these structures allows categorization of PmDsbA into the DsbA class exemplified by the archetypal Escherichia coli DsbA enzyme. We also present a crystal structure of PmDsbAC30S in complex with the peptide PWATCDS. The structure shows that the peptide binds non-covalently to the active site CXXC motif, the cis-Pro loop, and the hydrophobic groove adjacent to the active site of the enzyme. This high-resolution structural data provides a critical advance for future structure-based design of non-covalent peptidomimetic inhibitors. Such inhibitors would represent an entirely new antibacterial class that work by switching off the DSB virulence assembly machinery. PMID:24831013

  10. Production of phenylpyruvic acid from L-phenylalanine using an L-amino acid deaminase from Proteus mirabilis: comparison of enzymatic and whole-cell biotransformation approaches.

    PubMed

    Hou, Ying; Hossain, Gazi Sakir; Li, Jianghua; Shin, Hyun-Dong; Liu, Long; Du, Guocheng

    2015-10-01

    Phenylpyruvic acid (PPA) is an important organic acid that has a wide range of applications. In this study, the membrane-bound L-amino acid deaminase (L-AAD) gene from Proteus mirabilis KCTC 2566 was expressed in Escherichia coli BL21(DE3) and then the L-AAD was purified. After that, we used the purified enzyme and the recombinant E. coli whole-cell biocatalyst to produce PPA via a one-step biotransformation from L-phenylalanine. L-AAD was solubilized from the membrane and purified 52-fold with an overall yield of 13 %, which corresponded to a specific activity of 0.94?±?0.01 ?mol PPA min(-1)·mg(-1). Then, the biotransformation conditions for the pure enzyme and the whole-cell biocatalyst were optimized. The maximal production was 2.6?±?0.1 g·L(-1) (specific activity of 1.02?±?0.02 ?mol PPA min(-1)·mg(-1) protein, 86.7?±?5 % mass conversion rate, and 1.04 g·L(-1)·h(-1) productivity) and 3.3?±?0.2 g L(-1) (specific activity of 0.013?±?0.003 ?mol PPA min(-1)·mg(-1) protein, 82.5?±?4 % mass conversion rate, and 0.55 g·L(-1)·h(-1) productivity) for the pure enzyme and whole-cell biocatalyst, respectively. Comparative studies of the enzymatic and whole-cell biotransformation were performed in terms of specific activity, production, conversion, productivity, stability, need of external cofactors, and recycling. We have developed two eco-friendly and efficient approaches for PPA production. The strategy described herein may aid the biotransformational synthesis of other ?-keto acids from their corresponding amino acids. PMID:26109004

  11. Transcriptional Analysis of the MrpJ Network: Modulation of Diverse Virulence-Associated Genes and Direct Regulation of mrp Fimbrial and flhDC Flagellar Operons in Proteus mirabilis

    PubMed Central

    Bode, Nadine J.; Debnath, Irina; Kuan, Lisa; Schulfer, Anjelique; Ty, Maureen

    2015-01-01

    The enteric bacterium Proteus mirabilis is associated with a significant number of catheter-associated urinary tract infections (UTIs). Strict regulation of the antagonistic processes of adhesion and motility, mediated by fimbriae and flagella, respectively, is essential for disease progression. Previously, the transcriptional regulator MrpJ, which is encoded by the mrp fimbrial operon, has been shown to repress both swimming and swarming motility. Here we show that MrpJ affects an array of cellular processes beyond adherence and motility. Microarray analysis found that expression of mrpJ mimicking levels observed during UTIs leads to differential expression of 217 genes related to, among other functions, bacterial virulence, type VI secretion, and metabolism. We probed the molecular mechanism of transcriptional regulation by MrpJ using transcriptional reporters and chromatin immunoprecipitation (ChIP). Binding of MrpJ to two virulence-associated target gene promoters, the promoters of the flagellar master regulator flhDC and mrp itself, appears to be affected by the condensation state of the native chromosome, although both targets share a direct MrpJ binding site proximal to the transcriptional start. Furthermore, an mrpJ deletion mutant colonized the bladders of mice at significantly lower levels in a transurethral model of infection. Additionally, we observed that mrpJ is widely conserved in a collection of recent clinical isolates. Altogether, these findings support a role of MrpJ as a global regulator of P. mirabilis virulence. PMID:25847961

  12. [Obtaining antisera to Pseudomonas aeruginosa and Proteus antigens for performing immunoenzyme analyses in clinical practice].

    PubMed

    Bulava, G V; Ermolin, G A

    1985-01-01

    The data presented in this work indicate that specific antisera to P. aeruginosa and Proteus antigens can be produced by using extracts from these microorganisms, destroyed by ultrasonic treatment or by multiple freezing and thawing, for the immunization of rabbits. Blood serum samples from patients with purulent septic complications were studied for the presence of P. aeruginosa and Proteus antigens in ELISA with the use of peroxidase-labeled antibodies from antisera to P. aeruginosa and Proteus. This investigation revealed that during the first 3 days from the beginning of the clinical manifestations of the complications P. aeruginosa and Proteus antigens were detected in 86.4% and 83.4% of the patients, respectively. In the subsequent bacteriological study of wound discharge from these patients the corresponding microflora was detected. PMID:3920845

  13. [Immunoenzyme method of diagnosing suppurative-septic complications due to Pseudomonas aeruginosa and Proteus].

    PubMed

    Bulava, G V; Ermolin, G A; Grigor'ev, N I

    1984-06-01

    Two test systems, based on the inhibition of the reaction of enzyme-labeled antibodies and on the indirect reaction of enzyme-labeled antibodies, have been developed with a view to diagnosing purulent and septic complications caused by P. aeruginosa and Proteus in accordance with the results of the determination of the corresponding microbial antigens and antibodies to these antigens in the patients' blood. An increase in the levels of P. aeruginosa and Proteus antigens during the first 3 days of the development of complications and in the titers of antibodies on days 7-10 indicates that the purulent septic complication is caused by the given infective agent. A high correlation of the results obtained by bacteriological and serological diagnostic methods has been observed. PMID:6431720

  14. [Pseudomonas aeruginosa and Proteus antigenemia and antibody formation in mono- and mixed infections in patients with suppurative inflammatory diseases].

    PubMed

    Bulava, G V; Men'shikov, D D; Khvatov, V B

    1987-02-01

    P. aeruginosa and Proteus antigenemia and antibody production have been studied in 335 patients with purulent inflammatory diseases. The study has revealed that in the association of P. aeruginosa and Proteus with staphylococci and representatives of the family Enterobacteriaceae the level of antigenemia is considerably lower than in monoinfections or in the association of these microorganisms with streptococci. In mixed infections humoral immune response develops later than in cases of monoinfection. An important role of ecological and physiological relationships between microorganisms in the course of purulent processes and in their influence on the host has been confirmed. The use of the enzyme immunoassay in the clinical practice has made it possible to determine the etiological role of P. aeruginosa and Proteus in the development of suppurative-inflammatory diseases, to select adequate immunotherapy, including the use of anti-P. aeruginosa and anti-Proteus plasmas, and to evaluate the effectiveness of treatment. PMID:3107287

  15. Potential virulence factors of Proteus bacilli.

    PubMed Central

    Rózalski, A; Sidorczyk, Z; Kote?ko, K

    1997-01-01

    The object of this review is the genus Proteus, which contains bacteria considered now to belong to the opportunistic pathogens. Widely distributed in nature (in soil, water, and sewage), Proteus species play a significant ecological role. When present in the niches of higher macroorganisms, these species are able to evoke pathological events in different regions of the human body. The invaders (Proteus mirabilis, P. vulgaris, and P. penneri) have numerous factors including fimbriae, flagella, outer membrane proteins, lipopolysaccharide, capsule antigen, urease, immunoglobulin A proteases, hemolysins, amino acid deaminases, and, finally, the most characteristic attribute of Proteus, swarming growth, enabling them to colonize and survive in higher organisms. All these features and factors are described and commented on in detail. The questions important for future investigation of these facultatively pathogenic microorganisms are also discussed. PMID:9106365

  16. Extended spectrum beta lactamase producing Proteus penneri: a rare missed pathogen?

    PubMed

    Pandey, Anita; Verma, Himani; Asthana, Ashish K; Madan, Molly

    2014-01-01

    Indole negative Proteus species are invariably incorrectly identified as Proteus mirabilis, often missing out isolates of Proteus penneri. We report a case of extended spectrum beta lactamase producing and multidrug-resistant P. penneri isolated from pus from pressure sore of a patient of road traffic accident. Correct and rapid isolation and identification of such resistant pathogen are important as they are significant nosocomial threat. PMID:25118755

  17. Proteus at Sunset

    NASA Technical Reports Server (NTRS)

    2002-01-01

    The unique Proteus aircraft served as a test bed for NASA-sponsored flight tests designed to validate collision-avoidance technologies proposed for uninhabited aircraft. The tests, flown over southern New Mexico in March, 2002, used the Proteus as a surrogate uninhabited aerial vehicle (UAV) while three other aircraft flew toward the Proteus from various angles on simulated collision courses. Radio-based 'detect, see and avoid' equipment on the Proteus successfully detected the other aircraft and relayed that information to a remote pilot on the ground at Las Cruces Airport. The pilot then transmitted commands to the Proteus to maneuver it away from the potential collisions. The flight demonstration, sponsored by NASA Dryden Flight Research Center, New Mexico State University, Scaled Composites, the U.S. Navy and Modern Technology Solutions, Inc., were intended to demonstrate that UAVs can be flown safely and compatibly in the same skies as piloted aircraft.

  18. Isolation and characterization of Pseudomonas pseudomallei flagellin proteins.

    PubMed Central

    Brett, P J; Mah, D C; Woods, D E

    1994-01-01

    Flagellin proteins from several different strains of Pseudomonas pseudomallei have been isolated and purified to homogeneity by mechanical shearing and differential centrifugation techniques. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielded flagellin monomer protein bands with an estimated M(r) of 43,400. No lipopolysaccharide contamination of the purified protein preparations was detectable by silver staining of flagellin displayed on polyacrylamide gels and by Western immunoblotting with P. pseudomallei antilipopolysaccharide monoclonal antibody. NH2-terminal amino acid sequence analysis of the flagellin protein of P. pseudomallei 319a revealed significant homology with flagellins from Proteus mirabilis, Bordetella bronchiseptica, and Pseudomonas aeruginosa PAK. Rabbit polyclonal antiserum raised against the 319a flagellin protein reacted with 64 of 65 P. pseudomallei strains tested. The polyclonal antiserum proved effective in inhibiting the motility of these organisms in motility agar plates. Passive immunization studies demonstrated that 319a flagellin-specific antiserum was capable of protecting diabetic rats from challenge with a heterologous P. pseudomallei strain. Images PMID:7513308

  19. 21 CFR 524.1044h - Gentamicin, mometasone, and clotrimazole otic suspension.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...Malassezia pachydermatis ) and bacteria (Pseudomonas spp. [including P. aeruginosa ], coagulase-positive staphylococci, Enterococcus faecalis, Proteus mirabilis, and beta-hemolytic streptococci). (3) Limitations. Federal...

  20. 21 CFR 524.1044h - Gentamicin sulfate, mometasone furoate, clotrimazole otic suspension.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...Malassezia pachydermatis ) and bacteria (Pseudomonas spp. [including P. aeruginosa ], coagulase-positive staphylococci, Enterococcus faecalis, Proteus mirabilis, and beta-hemolytic streptococci). [66 FR 712, Jan. 4,...

  1. 21 CFR 524.1044h - Gentamicin sulfate, mometasone furoate, clotrimazole otic suspension.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...Malassezia pachydermatis ) and bacteria (Pseudomonas spp. [including P. aeruginosa ], coagulase-positive staphylococci, Enterococcus faecalis, Proteus mirabilis, and beta-hemolytic streptococci). [66 FR 712, Jan. 4,...

  2. 21 CFR 524.1044h - Gentamicin sulfate, mometasone furoate, clotrimazole otic suspension.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...Malassezia pachydermatis ) and bacteria (Pseudomonas spp. [including P. aeruginosa ], coagulase-positive staphylococci, Enterococcus faecalis, Proteus mirabilis, and beta-hemolytic streptococci). [66 FR 712, Jan. 4,...

  3. Proteus: Mythology to modern times

    PubMed Central

    Sellaturay, Senthy V.; Nair, Raj; Dickinson, Ian K.; Sriprasad, Seshadri

    2012-01-01

    Aims: It is common knowledge that proteus bacteria are associated with urinary tract infections and urinary stones. Far more interesting however, is the derivation of the word proteus. This study examines the origin of the word proteus, its mythological, historical and literary connections and evolution to present-day usage. Materials and Methods: A detailed search for primary and secondary sources was undertaken using the library and internet. Results: Greek mythology describes Proteus as an early sea-god, noted for being versatile and capable of assuming many different forms. In the 8th century BC, the ancient Greek poet, Homer, famous for his epic poems the Iliad and Odyssey, describes Proteus as a prophetic old sea-god, and herdsman of the seals of Poseidon, God of the Sea. Shakespeare re-introduced Proteus into English literature, in the 15th century AD, in the comedy The Two Gentleman of Verona, as one of his main characters who is inconstant with his affections. The ‘elephant man’ was afflicted by a severely disfiguring disease, described as ‘Proteus syndrome’. It is particularly difficult to distinguish from neurofibromatosis, due to its various forms in different individuals. The Oxford English Dictionary defines the word ‘protean’ as to mean changeable, variable, and existing in multiple forms. Proteus bacteria directly derive their name from the Sea God, due to their rapid swarming growth and motility on agar plates. They demonstrate versatility by secreting enzymes, which allow them to evade the host's defense systems. Conclusions: Thus proteus, true to its name, has had a myriad of connotations over the centuries. PMID:23450503

  4. The potential of selected Australian medicinal plants with anti-Proteus activity for the treatment and prevention of rheumatoid arthritis

    PubMed Central

    Cock, I. E.; Winnett, V.; Sirdaarta, J.; Matthews, B.

    2015-01-01

    Background: A wide variety of herbal medicines are used in indigenous Australian traditional medicinal systems to treat rheumatoid arthritis (RA) and inflammation. The current study was undertaken to test the ability of a panel of Australian plants with a history of the ethnobotanical usage in the treatment of inflammation for the ability to block the microbial trigger of RA. Materials and Methods: One hundred and six extracts from 40 plant species were investigated for the ability to inhibit the growth of the bacterial trigger of RA (Proteus mirabilis). The extracts were tested for toxicity in the Artemia nauplii bioassay. The most potent inhibitor of P. mirabilis growth was further analyzed by reversed-phase high performance liquid chromatography (RP-HPLC) coupled to high accuracy time-of-flight (TOF) mass spectroscopy. Results: Sixty-five of the 106 extracts tested (61.3%) inhibited the growth of P. The Aleurites moluccanus, Datura leichardtii, Eucalyptus major, Leptospermum bracteata, L. juniperium, Macadamia integriflora nut, Melaleuca alternifolia, Melaleuca quinquenervia, Petalostigma pubescens, P. triloculorae, P. augustifolium, Scaevola spinescens, Syzygiumaustrale, and Tasmannia lanceolata extracts were determined to be the most effective inhibitors of P. mirabilis growth, with minimum inhibitory concentration (MIC) values generally significantly below 1000 ?g/ml. T. lanceolata fruit extracts were the most effective P. mirabilis growth inhibitors, with a MIC values of 11 and 126 ?g/ml for the methanolic and aqueous extracts, respectively. Subsequent analysis of the T. lanceolata fruit extracts by RP-HPLC coupled to high-resolution TOF mass spectroscopy failed to detect resveratrol in either T. lanceolata fruit extract. However, the resveratrol glycoside piceid and 2 combretastatin stilbenes (A-1 and A-4) were detected in both T. lanceolata fruit extracts. With the exception of the Eucalyptus and Syzygium extracts, all extracts exhibiting Proteus inhibitory activity were also shown to be nontoxic, or of low toxicity in the Artemia nauplii bioassay. Conclusions: The low toxicity of these extracts and their inhibitory bioactivity against Proteus spp. indicate their potential in blocking the onset of rheumatoid arthritis. PMID:26109767

  5. Proteus virulence: involvement of the pore forming alpha-hemolysin (a short review).

    PubMed

    Tóth, V; Emódy, L

    2000-01-01

    The genus Proteus belongs to the tribe of Proteae in the family of Enterobacteriaceae, and consists of five species: P. mirabilis, P. vulgaris, P. morganii, P. penneri and P. myxofaciens. They are distinguished from the rest of Enterobacteriaceae by their ability to deaminate phenylalanine and tryptophane. They hydrolyze urea and gelatin and fail to ferment lactose, mannose, dulcitol and malonate; and do not form lysine and arginine decarboxylase or beta-galactosidase [1]. Colonies produce distinct "burned chocolate" odor and frequently show the characteristics of swarming motility on solid media. P. mirabilis, P. vulgaris and P. morganii are widely recognized human pathogens. They have been isolated from urinary tract infections, wounds, ear, and nosocomial bacteremic infections, often in immuncompromised patients [2-6]. P. myxofaciens has no clinical interest to this time. P. penneri as species nova was nominated by the recommendation of Hickman and co-workers [7]. Formerly it was recognized as P. vulgaris biogroup 1 or indole negative P. vulgaris [8, 9]. Although it has been less commonly isolated from clinical samples than the other three human pathogenic Proteus species, it has nevertheless been connected with infections of the urinary tract, wounds and has been isolated from the feces of both healthy and diarrheic individuals [10-12]. Potential virulence factors responsible for virulence of Proteae are: IgA protease, urease, type3 fimbriae associated with MR/K haemagglutinins of at least two antigenic types, endotoxin, swarming motility and HlyA and/or HpmA type hemolysins [for review see ref. 13]. In the followings we give a survey of accumulated concepts about the position and characteristics of HlyA type alpha-hemolysins both in general and with emphasis on virulence functions in the tribe of Proteae. PMID:11056765

  6. Myocardial Fat Overgrowth in Proteus Syndrome

    PubMed Central

    Hannoush, H; Sachdev, V; Brofferio, A; Arai, AE; LaRocca, G; Sapp, J; Sidenko, S; Brenneman, C; Biesecker, LG; Keppler-Noreuil, KM

    2014-01-01

    Proteus syndrome is a rare, mosaic disorder with asymmetric and distorting overgrowth of the skeletal system, skin, and adipose tissues. Cardiac abnormalities are rare in this syndrome and only two prior cases have been reported. Many patients with PS followed at our institution underwent transthoracic echocardiograms for preoperative evaluation or as work-up for associated pulmonary disease. Some were noted to have prominent, focal echodense areas in the myocardium. We further investigated cardiac findings in a cohort of children and adult patients with PS. Patients with abnormal echocardiograms were referred for cardiac magnetic resonance imaging, Holter monitoring, and exercise treadmill testing. Twenty children and adults with PS, age 24 months to 50 years old, underwent transthoracic echocardiograms. Seven patients (35%) had focal bright echodense areas within the myocardium suggesting fatty infiltration. The majority of patients had significant involvement of the interventricular septum. The cardiac characteristics of all patients with fatty infiltration on transthoracic echocardiograms were compared to Proteus patients without these findings. There were no significant differences in chamber sizes, mass, systolic or diastolic function. No increased risk of conduction defects or arrhythmias was found. This study shows that abnormal fat overgrowth is a common finding in the myocardium in patients with Proteus syndrome; however, it is not associated with functional derangements or arrhythmias. Further evaluation of a larger number of Proteus patients is needed in order to determine the frequency and prognosis of cardiac involvement. PMID:25377688

  7. An explanatory model to validate the way water activity rules periodic terrace generation in Proteus mirabilis swarm

    E-print Network

    Frénod, Emmanuel

    by hundreds of flagella can also be seen (B). The third panel (C) displays a close up of the peripheral edge motion of swarmer cell flagella making it moves while the medium is gradually colonized. The last panel concerns the cell size distribution and was pointed out in Matsuyama et al. [33]. As for space distribution

  8. User Manual for the PROTEUS Mesh Tools

    SciTech Connect

    Smith, Micheal A.; Shemon, Emily R.

    2015-06-01

    This report describes the various mesh tools that are provided with the PROTEUS code giving both descriptions of the input and output. In many cases the examples are provided with a regression test of the mesh tools. The most important mesh tools for any user to consider using are the MT_MeshToMesh.x and the MT_RadialLattice.x codes. The former allows the conversion between most mesh types handled by PROTEUS while the second allows the merging of multiple (assembly) meshes into a radial structured grid. Note that the mesh generation process is recursive in nature and that each input specific for a given mesh tool (such as .axial or .merge) can be used as “mesh” input for any of the mesh tools discussed in this manual.

  9. Proteus syndrome: A rare case report

    PubMed Central

    Talari, Keerthi; Subbanna, Praveen Kumar Arinaganhalli; Amalnath, Deepak; Suri, Subrahmanyam Dharanitragada Krishna

    2012-01-01

    Proteus syndrome (PS) is a rare hamartomatous disorder characterized by various cutaneous and subcutaneous lesions, including vascular malformations, lipomas, hyperpigmentation, and several types of nevi. Partial gigantism with limb or digital overgrowth is pathognomonic of PS. We report a rare case of PS in a 50-year-old man who presented with inferior wall myocardial infarction and was incidentally detected to have hypertrophy of index and middle fingers of both the hands. PMID:23716948

  10. Proteus syndrome: what the anesthetist should know.

    PubMed

    Sethi, Divya

    2015-08-01

    Proteus syndrome (PS), a rare hamartomatous disorder, manifests itself in asymmetric and disproportionate overgrowth of multiple body tissues. Because of complexity of the disorder, the anesthetic problems encountered during patients' perioperative management are very varied. We discuss the case of a 14-year-old adolescent boy diagnosed with PS who underwent corrective osteotomy of right knee joint under subarachnoid block. The salient points the anesthetists need to be aware of while caring for patients with PS are highlighted. PMID:25921368

  11. Proteus syndrome and hypothyroidism. An unusual association.

    PubMed

    Ali, Manzoor A; Yaseen, Hakam A; Muhammed, Muhammed A

    2014-09-01

    We present a case of a 3˝-year-old girl diagnosed as Proteus syndrome with severe cosmetic disfigurement-macrodactyly, hemi-hypertrophy of the face and limbs, megalencephaly, lymph edema of both hands and feet along with severe global developmental delay. She was found to have severe recalcitrant epilepsy and also primary hypothyroidism; the association of which is not mentioned in the previous literature. PMID:25228186

  12. GENES ASSOCIATED WITH OPENING AND SENESCENCE OF MIRABILIS JALAPA FLOWERS

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A modest ethylene climacteric accompanies flower senescence in Mirabilis jalapa L., and exogenous ethylene accelerates the process. However, inhibitors of ethylene action and synthesis have little effect on the life-span of these ephemeral flowers. Treatment with '-amanitin, an inhibitor of DNA-de...

  13. The Proteus Cabinet, or "We Are Here but Not Here"

    ERIC Educational Resources Information Center

    Nield, Sophie

    2008-01-01

    In the early nineteenth century, there were three stage illusions in which a magician could cause a person to disappear. In one of these, the Proteus Cabinet, participants would enter a box, and simply vanish. As the designers of the Proteus Cabinet said of them, they were "Here, but not Here." My essay explores this concept in relation to…

  14. Mirabilis jalapa mottle virus: a new carlavirus infecting four o'clocks.

    PubMed

    Hatlestad, Gregory J; Elam, Lee; Gonzalez, Antonio; Lloyd, Alan M

    2011-11-01

    Analysis of a next-generation sequence dataset from Mirabilis jalapa resulted in the discovery of a novel virus in the genus Carlavirus (family Betaflexiviridae), mirabilis jalapa mottle virus (MjMV). The complete genome of MjMV was determined to consist of 8315 nucleotides (nt), with the six open reading frames indicative of carlaviruses. MjMV is most similar to kalanchoe latent virus (60% identity) and lily symptomless virus (59% identity). The virus can be transmitted mechanically to Mirabilis, but thus far MjMV has only been shown to infect Mirabilis jalapa, causing a slight leaf mottling and leaf wrinkling phenotype. PMID:21915718

  15. Proteus Syndrome: Report of a Case with Developmental Glaucoma

    PubMed Central

    Yuksel, Nursen; Sarman, Hakan; Bayramgurler, Dilek

    2014-01-01

    The purpose of this study was to report developmental glaucoma and pseudopapilledema in a patient with Proteus syndrome. We defined the presence of developmental glaucoma, right pseudopapilledema and myopia in a 4.5-year-old patient with Proteus syndrome. Marked right hemihypertrophy, lipoma, macrodactyly, and asymmetry of the limbs were observed on systemic examination. A cavernoma was also detected in magnetic resonance imaging of the brain. The patient underwent bilateral goniotomy surgery due to glaucoma. The surgical outcomes were satisfactory in both eyes. In conclusions developmental glaucoma and pseudopapilledema might be associated with Proteus syndrome. PMID:24882963

  16. Proteus syndrome: report of a case with developmental glaucoma.

    PubMed

    Sarman, Zuleyha Sik; Yuksel, Nursen; Sarman, Hakan; Bayramgurler, Dilek

    2014-06-01

    The purpose of this study was to report developmental glaucoma and pseudopapilledema in a patient with Proteus syndrome. We defined the presence of developmental glaucoma, right pseudopapilledema and myopia in a 4.5-year-old patient with Proteus syndrome. Marked right hemihypertrophy, lipoma, macrodactyly, and asymmetry of the limbs were observed on systemic examination. A cavernoma was also detected in magnetic resonance imaging of the brain. The patient underwent bilateral goniotomy surgery due to glaucoma. The surgical outcomes were satisfactory in both eyes. In conclusions developmental glaucoma and pseudopapilledema might be associated with Proteus syndrome. PMID:24882963

  17. Catharanthus mosaic virus: A potyvirus from a gymnosperm, Welwitschia mirabilis.

    PubMed

    Koh, Shu Hui; Li, Hua; Admiraal, Ryan; Jones, Michael G K; Wylie, Stephen J

    2015-05-01

    A virus from a symptomatic plant of the gymnosperm Welwitschia mirabilis Hook. growing as an ornamental plant in a domestic garden in Western Australia was inoculated to a plant of Nicotiana benthamiana where it established a systemic infection. The complete genome sequence of 9636 nucleotides was determined using high-throughput and Sanger sequencing technologies. The genome sequence shared greatest identity (83% nucleotides and 91% amino acids) with available partial sequences of catharanthus mosaic virus, indicating that the new isolate belonged to that taxon. Analysis of the phylogeny of the complete virus sequence placed it in a monotypic group in the genus Potyvirus. This is the first record of a virus from W. mirabilis, the first complete genome sequence of catharanthus mosaic virus determined, and the first record from Australia. This finding illustrates the risk to natural and managed systems posed by the international trade in live plants and propagules, which enables viruses to establish in new regions and infect new hosts. PMID:25804761

  18. Intracellular Microrheology of Motile Amoeba proteus

    PubMed Central

    Rogers, Salman S.; Waigh, Thomas A.; Lu, Jian R.

    2008-01-01

    The motility of Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly developed particle tracking software, a fast digital camera, and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion at short timescales, corresponding to thermal motion in a viscoelastic medium, and superdiffusive motion at long timescales due to the convection of the cytoplasm. Subdiffusive motion was characterized by a rheological scaling exponent of 3/4 in the cortex, indicative of the semiflexible dynamics of the actin fibers. We observed shear-thinning in the flowing endoplasm, where exponents increased with increasing flow rate; i.e., the endoplasm became more fluid-like. The rheology of the cortex is found to be isotropic, reflecting an isotropic actin gel. A clear difference was seen between cortical and endoplasmic layers in terms of both viscoelasticity and flow velocity, where the profile of the latter is close to a Poiseuille flow for a Newtonian fluid. PMID:18192370

  19. Intracellular microrheology of motile Amoeba proteus

    E-print Network

    Salman S. Rogers; Thomas A. Waigh; Jian R. Lu

    2007-10-12

    The motility of motile Amoeba proteus was examined using the technique of passive particle tracking microrheology, with the aid of newly-developed particle tracking software, a fast digital camera and an optical microscope. We tracked large numbers of endogeneous particles in the amoebae, which displayed subdiffusive motion at short time scales, corresponding to thermal motion in a viscoelastic medium, and superdiffusive motion at long time scales due to the convection of the cytoplasm. Subdiffusive motion was characterised by a rheological scaling exponent of 3/4 in the cortex, indicative of the semiflexible dynamics of the actin fibres. We observed shear-thinning in the flowing endoplasm, where exponents increased with increasing flow rate; i.e. the endoplasm became more fluid-like. The rheology of the cortex is found to be isotropic, reflecting an isotropic actin gel. A clear difference was seen between cortical and endoplasmic layers in terms of both viscoelasticity and flow velocity, where the profile of the latter is close to a Poiseuille flow for a Newtonian fluid.

  20. A Case of Concurrent Proteus Syndrome and Hemophilia A

    PubMed Central

    Hashemieh, Mozhgan; Mansoori, Bahar; Tavakoli, Reza; Sheibani, Koroush

    2012-01-01

    Background Proteus syndrome is a very rare condition with less than 100 confirmed cases reported worldwide. We report a case of Proteus syndrome in a two-year-old male who has hemophilia A comorbidity. Case Presentation A two-year-old male patient was admitted with the chief complaint of severe bleeding in mouth cavity after trauma for two weeks. At admission he was found to have petechiae on buccal mucosa and fecal discoloration due to GI bleeding. We noted multiple abnormalities in his musculoskeletal system and skin. He had lymph edema in left leg, hemihypertrophy, macrodactyly in both foots and macrocephaly. With the history of severe bleeding and recurrent blood product transfusion, we suspected a hemorrhagic disorder. The reduced level of Factor VIII activity confirmed the diagnosis of hemophilia A. Considering patient's various musculoskeletal abnormalities according to the diagnostic criteria and after ruling out similar disorders the diagnosis of Proteus syndrome was established. Conclusion Because of the variability of clinical features, Proteus syndrome can be confused with other disorders of multiple tissue overgrowth. Our case of Proteus syndrome, who had hemophilia A comorbidity outlines the challenges in diagnosis of such rare combination of diseases. PMID:23056896

  1. INFECTIVE ORGANISMS IN THE CYTOPLASM OF AMOEBA PROTEUS

    PubMed Central

    Roth, L. E.; Daniels, E. W.

    1961-01-01

    Evidence from electron and phase microscopy is given which shows that infective organisms are present in the cytoplasm of Amoeba proteus. Vesicles containing living organisms have been observed after repeated washing and starvation of the amebae for a period of 2 weeks. Exposure to ?-radiation in conjunction with starvation, repeated washing, isolation of single amebae, refeeding with contaminant-free Tetrahymena, and clone selection has produced clones with reduced cytoplasmic infection. These findings are discussed in regard to the autoradiographic studies of other investigators on Amoeba proteus. The controversies over whether DNA and RNA are synthesized in the cytoplasm may be resolved by the finding of cytoplasmic infection. PMID:13743844

  2. El universo de Einstein: 1905 - annus mirabilis - 2005

    E-print Network

    Alejandro Gangui

    2007-06-18

    Year 2005 was the World Year of Physics, and it was commemorated worldwide as the 100th anniversary of Albert Einstein's legendary works in which the foundations of at least three areas of modern physics were laid: statistical mechanics, quantum mechanics and special relativity. These works turned year 1905 in what deservedly was called the "miraculous year" (annus mirabilis) of Einstein. However, the influence of this great mind was not limited to science. As it is well known, his fingerprint remains engraved in many aspects of our daily life. Because of that, in this work we have tried to cover almost all the subjects in which Einstein was involved during the last century and, of course, try and reveal the future projection his actions will have in this new century that has just begun. Among the subjects covered in the volume one can find the possible connection between Einstein and the artistic vanguard movement, his religiosity, his days in Argentina, the Nobel Prize, the Einstein-Freud correspondence, his pacifism and the nuclear energy, science and politics under the Second Empire and the Weimar Republic, and even a description of his patents and experiments, the studies performed to search for "built-in" features in his brain and the doubts many had regarding whether Einstein was a genius or a crank. Also included are essays on Einstein and Borges, philosophy and relativity, Mach's principle, the relation among science, arts and humanities, and, closer to Einstein's own theoretical research, the twin paradox, chance and quantum mechanics, the arrow of time, gravitational lenses, string theory, holography and black holes, gravitomagnetism, superconductivity, gravitational waves, the photoelectric effect and the alternative theories for gravitation.

  3. Genes associated with opening and senescence of the ephemeral flowers of Mirabilis jalapa

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A modest ethylene climacteric accompanies flower senescence in Mirabilis jalapa L., and exogenous ethylene accelerates the process. However, inhibitors of ethylene action and synthesis have little effect on the life-span of these ephemeral flowers. Treatment with a-amanitin, an inhibitor of DNA-depe...

  4. Incomplete dominance for flower colour in Mirabilis jalapa R1 Red pigment

    E-print Network

    Shore, Joel S.

    R1R1 R1R2 R2R2 Incomplete dominance for flower colour in Mirabilis jalapa R1 Red pigment R2 No pigment #12;Alcohol dehydrogenase in pollen of Asclepias syriaca (milkweed) Co-dominance at an isozyme. Since both mutants are recessive, there is in essence a "functional" copy of each gene so purple pigment

  5. Proteus syndrome: A rare cause of gigantic limb

    PubMed Central

    Chakrabarti, Nandini; Chattopadhyay, Chandan; Bhuban, Majhi; Pal, Salil Kumar

    2014-01-01

    A congenital disorder with variable manifestations, including partial gigantism of the hands and feet with hypertrophy of soles, nevi, hemihypertrophy, gynecomastia, macrocephaly and other skull abnormalities, and abdominal lipomatosis. The cause is unknown, although a genetic origin, generally of autosomal-dominant transmission, has been conjectured. Symptoms can be treated, but there is no known cure. We present the case of a young male with grotesque overgrowth of the right lower limb, splenomegaly and multiple nevi. Angiography revealed venous malformation within the limb. The findings are in conformity to the criteria for the Proteus syndrome. PMID:24860761

  6. Proteus syndrome: A rare cause of gigantic limb.

    PubMed

    Chakrabarti, Nandini; Chattopadhyay, Chandan; Bhuban, Majhi; Pal, Salil Kumar

    2014-04-01

    A congenital disorder with variable manifestations, including partial gigantism of the hands and feet with hypertrophy of soles, nevi, hemihypertrophy, gynecomastia, macrocephaly and other skull abnormalities, and abdominal lipomatosis. The cause is unknown, although a genetic origin, generally of autosomal-dominant transmission, has been conjectured. Symptoms can be treated, but there is no known cure. We present the case of a young male with grotesque overgrowth of the right lower limb, splenomegaly and multiple nevi. Angiography revealed venous malformation within the limb. The findings are in conformity to the criteria for the Proteus syndrome. PMID:24860761

  7. Mechanics and control of the cytoskeleton in Amoeba proteus.

    PubMed Central

    Dembo, M

    1989-01-01

    Many models of the cytoskeletal motility of Amoeba proteus can be formulated in terms of the theory of reactive interpenetrating flow (Dembo and Harlow, 1986). We have devised numerical methodology for testing such models against the phenomenon of steady axisymmetric fountain flow. The simplest workable scheme revealed by such tests (the minimal model) is the main preoccupation of this study. All parameters of the minimal model are determined from available data. Using these parameters the model quantitatively accounts for the self assembly of the cytoskeleton of A. proteus: for the formation and detailed morphology of the endoplasmic channel, the ectoplasmic tube, the uropod, the plasma gel sheet, and the hyaline cap. The model accounts for the kinematics of the cytoskeleton: the detailed velocity field of the forward flow of the endoplasm, the contraction of the ectoplasmic tube, and the inversion of the flow in the fountain zone. The model also gives a satisfactory account of measurements of pressure gradients, measurements of heat dissipation, and measurements of the output of useful work by amoeba. Finally, the model suggests a very promising (but still hypothetical) continuum formulation of the free boundary problem of amoeboid motion. by balancing normal forces on the plasma membrane as closely as possible, the minimal model is able to predict the turgor pressure and surface tension of A. proteus. Several dynamical factors are crucial to the success of the minimal model and are likely to be general features of cytoskeletal mechanics and control in amoeboid cells. These are: a constitutive law for the viscosity of the contractile network that includes an automatic process of gelation as the network density gets large; a very vigorous cycle of network polymerization and depolymerization (in the case of A. proteus, the time constant for this reaction is approximately 12 s); control of network contractility by a diffusible factor (probably calcium ion); and control of the adhesive interaction between the cytoskeleton and the inner surface of the plasma membrane. Images FIGURE 1 FIGURE 2 FIGURE 7 PMID:2765645

  8. Proteus syndrome: Clinical diagnosis of a series of cases

    PubMed Central

    Alves, Cresio; Acosta, Angelina X.; Toralles, Maria Betânia P.

    2013-01-01

    Objectives: This paper describes the clinical diagnosis of Proteus syndrome (PS) in children referred for evaluation of asymmetric disproportionate overgrowth. Materials and Methods: Retrospective, descriptive, cross-sectional study conducted from January 1998 to December 2010. Results: During the study period, 2011 new patients were evaluated. Thirteen (0.65%) patients presented features suggestive of PS. These patients were formally evaluated based on the revised diagnostic criteria proposed by Biesecker. The mean age was 6.92 ± 5.1 years. Ten patients (76.9%) were females. All subjects had asymmetric disproportionate overgrowth. Other dysmorphic features were as follows: macrodactily (84.6%); linear epidermal nevus (41.6%); hemangioma (30.7%); and lipoma (23%). Six patients fulfilled the diagnostic criteria for PS. Conclusions: The diagnostic rate of only 46.1% of patients with PS confirms the diagnostic difficulties and the need for continuous monitoring and periodic review of these patients since the clinical manifestations of this syndrome become more evident with aging. Molecular tests may help the differential diagnosis of Proteus syndrome when they became commercially available. PMID:24381883

  9. Convergence acceleration of the Proteus computer code with multigrid methods

    NASA Technical Reports Server (NTRS)

    Demuren, A. O.; Ibraheem, S. O.

    1995-01-01

    This report presents the results of a study to implement convergence acceleration techniques based on the multigrid concept in the two-dimensional and three-dimensional versions of the Proteus computer code. The first section presents a review of the relevant literature on the implementation of the multigrid methods in computer codes for compressible flow analysis. The next two sections present detailed stability analysis of numerical schemes for solving the Euler and Navier-Stokes equations, based on conventional von Neumann analysis and the bi-grid analysis, respectively. The next section presents details of the computational method used in the Proteus computer code. Finally, the multigrid implementation and applications to several two-dimensional and three-dimensional test problems are presented. The results of the present study show that the multigrid method always leads to a reduction in the number of iterations (or time steps) required for convergence. However, there is an overhead associated with the use of multigrid acceleration. The overhead is higher in 2-D problems than in 3-D problems, thus overall multigrid savings in CPU time are in general better in the latter. Savings of about 40-50 percent are typical in 3-D problems, but they are about 20-30 percent in large 2-D problems. The present multigrid method is applicable to steady-state problems and is therefore ineffective in problems with inherently unstable solutions.

  10. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... early mortality caused by Escherichia coli. Salmonella typhimurium, and Pseudomonas aeruginosa that are... in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli... old for treatment of porcine colibacillosis caused by strains of E. coli sensitive to gentamicin....

  11. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... early mortality caused by Escherichia coli. Salmonella typhimurium, and Pseudomonas aeruginosa that are... in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli... old for treatment of porcine colibacillosis caused by strains of E. coli sensitive to gentamicin....

  12. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... early mortality caused by Escherichia coli. Salmonella typhimurium, and Pseudomonas aeruginosa that are... in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli... old for treatment of porcine colibacillosis caused by strains of E. coli sensitive to gentamicin....

  13. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... early mortality caused by Escherichia coli. Salmonella typhimurium, and Pseudomonas aeruginosa that are... in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli... old for treatment of porcine colibacillosis caused by strains of E. coli sensitive to gentamicin....

  14. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... early mortality caused by Escherichia coli. Salmonella typhimurium, and Pseudomonas aeruginosa that are... in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli... old for treatment of porcine colibacillosis caused by strains of E. coli sensitive to gentamicin....

  15. Pseudomonas 2007 Meeting Review

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas is an important genus of bacteria. Pseudomonas aeruginosa is the third most common nosocomial pathogen in our society, associated with chronic and eventually fatal lung disease in cystic fibrosis patients, while Pseudomonas syringae species are prominent plant pathogens. The fluorescen...

  16. Clinical differentiation between Proteus syndrome and hemihyperplasia: description of a distinct form of hemihyperplasia.

    PubMed

    Biesecker, L G; Peters, K F; Darling, T N; Choyke, P; Hill, S; Schimke, N; Cunningham, M; Meltzer, P; Cohen, M M

    1998-10-01

    Proteus syndrome is a rare and highly variable hamartomatous syndrome that can affect multiple organ systems. It is characterized by hyperplastic lesions of connective tissue, vascular malformations, linear verrucous epidermal nevi, and hyperostoses. The cause of the disorder is unknown, but the current working hypothesis is that it is caused by a mosaic alteration that leads to a highly variable phenotype, equal sex ratio, sporadic occurrence, and discordant monozygotic twins. Herein we describe our experience with 18 patients with a referring diagnosis of Proteus syndrome. It was found that imaging studies are very useful for the characterization of the syndrome. One finding was that splenic hyperplasia can be a manifestation of Proteus syndrome. Analysis of the clinical data shows that Proteus syndrome is frequently confused with "hemihyperplasia." A distinct subtype of hemihyperplasia is defined that includes static or mildly progressive hemihyperplasia and multiple lipomata. PMID:9781913

  17. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...immunofluorescent reagents), derived from the bacterium Proteus vulgaris used in agglutination...antibodies to rickettsia (virus-like bacteria) in serum. Test results aid in the diagnosis of diseases caused by bacteria belonging to the genus...

  18. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...immunofluorescent reagents), derived from the bacterium Proteus vulgaris used in agglutination...antibodies to rickettsia (virus-like bacteria) in serum. Test results aid in the diagnosis of diseases caused by bacteria belonging to the genus...

  19. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...immunofluorescent reagents), derived from the bacterium Proteus vulgaris used in agglutination...antibodies to rickettsia (virus-like bacteria) in serum. Test results aid in the diagnosis of diseases caused by bacteria belonging to the genus...

  20. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...immunofluorescent reagents), derived from the bacterium Proteus vulgaris used in agglutination...antibodies to rickettsia (virus-like bacteria) in serum. Test results aid in the diagnosis of diseases caused by bacteria belonging to the genus...

  1. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...immunofluorescent reagents), derived from the bacterium Proteus vulgaris used in agglutination...antibodies to rickettsia (virus-like bacteria) in serum. Test results aid in the diagnosis of diseases caused by bacteria belonging to the genus...

  2. Unilateral hypertrophic skin lesions, hemimegalencephaly, and meningioma: The many faces of Proteus syndrome

    PubMed Central

    Lal, Niharika R.; Bandyopadhyay, Debabrata; Sarkar, Asim K.

    2015-01-01

    Proteus syndrome is a rare condition with a wide spectrum of abnormalities. It is characterized by hamartomatous malformations involving multiple organs. Serious complications may ensue, such as pulmonary embolism, cystic lung disease, and various neoplasms such as parotid adenomas, ovarian cystadenomas, and meningiomas. We report here a case of Proteus syndrome in a 21-year-old woman who had facial hemihypertrophy, cerebriform plantar hyperplasia, hemimegalencephaly, and meningioma for the rarity of the entity. PMID:26500869

  3. Unilateral hypertrophic skin lesions, hemimegalencephaly, and meningioma: The many faces of Proteus syndrome.

    PubMed

    Lal, Niharika R; Bandyopadhyay, Debabrata; Sarkar, Asim K

    2015-01-01

    Proteus syndrome is a rare condition with a wide spectrum of abnormalities. It is characterized by hamartomatous malformations involving multiple organs. Serious complications may ensue, such as pulmonary embolism, cystic lung disease, and various neoplasms such as parotid adenomas, ovarian cystadenomas, and meningiomas. We report here a case of Proteus syndrome in a 21-year-old woman who had facial hemihypertrophy, cerebriform plantar hyperplasia, hemimegalencephaly, and meningioma for the rarity of the entity. PMID:26500869

  4. Progressive Overgrowth of the Cerebriform Connective Tissue Nevus in Patients with Proteus Syndrome

    PubMed Central

    Beachkofsky, Thomas M.; Sapp, Julie C.; Biesecker, Leslie G.; Darling, Thomas N.

    2011-01-01

    Background Proteus syndrome is a rare overgrowth disorder that almost always affects the skin. Objective Our purpose was to evaluate progression of skin lesions in patients with Proteus syndrome. Methods Skin findings were documented in 36 patients with Proteus syndrome. Progression of skin lesions in 16 of these patients was assessed by comparing photographs obtained on repeat visits for an average total duration of 53 months. Results The skin lesion most characteristic of Proteus syndrome, the cerebriform connective tissue nevus showed progression in 13 children but not in 3 adults. The cerebriform connective tissue nevus progressed by expansion into previously uninvolved skin, increased thickness, and development of new lesions. Lipomas increased in size and/or number in 8/10 children with lipomas. In contrast, epidermal nevi and vascular malformations generally did not spread or increase in number. Limitations Only 3 adults with Proteus syndrome were evaluated longitudinally. Conclusion The cerebriform connective tissue nevus in Proteus syndrome grows throughout childhood but tends to remain stable in adulthood. PMID:20709429

  5. A severe and rapidly progressive case of proteus syndrome in a neonate who presented with unilateral hydrocephalus apart from other typical features of the proteus syndrome.

    PubMed

    Kumar, Rakesh; Bhagat, Puja

    2012-07-01

    Proteus syndrome is a rare hamartomatous disorder affecting multiple tissues and manifesting itself in a variety of ways. The understanding of the complete spectrum of clinical features, the natural clinical course of the disease and the proper management of such a rare but highly variable syndrome depend heavily on experiences gathered by previously reported cases. We present an unusually severely affected and rapidly progressive case of proteus syndrome in a neonate who presented with craniofacial hemihypertrophy, subcutaneous masses, capillary hemangioma, varicose veins, epidermal nevi and macrodactyly. The cranial ultrasonogram revealed unilateral hydrocephalus with partial obstruction of the foramen of monro. PMID:24027714

  6. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...Staphylococcus spp., Escherichia coli, Proteus mirabilis, and Pasteurella... spp., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus ...spp., Enterococcus spp., and E. coli. ; infections associated with...

  7. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...Staphylococcus spp., Escherichia coli, Proteus mirabilis, and Pasteurella... spp., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus ...spp., Enterococcus spp., and E. coli. ; infections associated with...

  8. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...Staphylococcus spp., Escherichia coli, Proteus mirabilis, and Pasteurella... spp., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus ...spp., Enterococcus spp., and E. coli. ; infections associated with...

  9. Thoracolumbar Scoliosis in a Patient With Proteus Syndrome

    PubMed Central

    Li, Zheng; Shen, Jianxiong; Liang, Jinqian

    2015-01-01

    Abstract The Proteus syndrome (PS) is a complex and rare congenital hamartomatous condition with a wide range of malformations. Little is reported about spinal deformity associated with this syndrome. This study presents a case of scoliosis occurring in the setting of PS and explores the possible mechanisms between the 2 diseases. The patient is a 17-year-old Chinese female with scoliosis and hemihypertrophy of the right upper and lower extremity as well as exostosis of the right lower leg joint including the hip, knee, ankle, and toes. These manifestations were suggestive of PS. She underwent a posterior correction at thoracic 2-lumbar 4 (T5–L4) levels, using the Moss-SI spinal system. At 3-month follow-ups, the patient was clinically pain free and well balanced. Plain radiographs showed solid spine fusion with no loss of deformity correction. The severity of scoliosis in PS is progressively aggravated and the correction of the extensive spinal deformities is generally difficult. Therefore, early diagnosis is required for adequate interdisciplinary treatment. PMID:25654373

  10. Proteus: a reconfigurable computational network for computer vision

    NASA Astrophysics Data System (ADS)

    Haralick, Robert M.; Somani, Arun K.; Wittenbrink, Craig M.; Johnson, Robert; Cooper, Kenneth; Shapiro, Linda G.; Phillips, Ihsin T.; Hwang, Jenq N.; Cheung, William; Yao, Yung H.; Chen, Chung-Ho; Yang, Larry; Daugherty, Brian; Lorbeski, Bob; Loving, Kent; Miller, Tom; Parkins, Larye; Soos, Steven L.

    1992-04-01

    The Proteus architecture is a highly parallel MIMD, multiple instruction, multiple-data machine, optimized for large granularity tasks such as machine vision and image processing The system can achieve 20 Giga-flops (80 Giga-flops peak). It accepts data via multiple serial links at a rate of up to 640 megabytes/second. The system employs a hierarchical reconfigurable interconnection network with the highest level being a circuit switched Enhanced Hypercube serial interconnection network for internal data transfers. The system is designed to use 256 to 1,024 RISC processors. The processors use one megabyte external Read/Write Allocating Caches for reduced multiprocessor contention. The system detects, locates, and replaces faulty subsystems using redundant hardware to facilitate fault tolerance. The parallelism is directly controllable through an advanced software system for partitioning, scheduling, and development. System software includes a translator for the INSIGHT language, a parallel debugger, low and high level simulators, and a message passing system for all control needs. Image processing application software includes a variety of point operators neighborhood, operators, convolution, and the mathematical morphology operations of binary and gray scale dilation, erosion, opening, and closing.

  11. Benchmark Evaluation of HTR-PROTEUS Pebble Bed Experimental Program

    DOE PAGESBeta

    Bess, John D.; Montierth, Leland; Köberl, Oliver; Snoj, Luka

    2014-10-09

    Benchmark models were developed to evaluate 11 critical core configurations of the HTR-PROTEUS pebble bed experimental program. Various additional reactor physics measurements were performed as part of this program; currently only a total of 37 absorber rod worth measurements have been evaluated as acceptable benchmark experiments for Cores 4, 9, and 10. Dominant uncertainties in the experimental keff for all core configurations come from uncertainties in the ˛ł?U enrichment of the fuel, impurities in the moderator pebbles, and the density and impurity content of the radial reflector. Calculations of keff with MCNP5 and ENDF/B-VII.0 neutron nuclear data are greatermore »than the benchmark values but within 1% and also within the 3? uncertainty, except for Core 4, which is the only randomly packed pebble configuration. Repeated calculations of keff with MCNP6.1 and ENDF/B-VII.1 are lower than the benchmark values and within 1% (~3?) except for Cores 5 and 9, which calculate lower than the benchmark eigenvalues within 4?. The primary difference between the two nuclear data libraries is the adjustment of the absorption cross section of graphite. Simulations of the absorber rod worth measurements are within 3? of the benchmark experiment values. The complete benchmark evaluation details are available in the 2014 edition of the International Handbook of Evaluated Reactor Physics Benchmark Experiments.« less

  12. Benchmark Evaluation of HTR-PROTEUS Pebble Bed Experimental Program

    SciTech Connect

    Bess, John D.; Montierth, Leland; Köberl, Oliver; Snoj, Luka

    2014-10-09

    Benchmark models were developed to evaluate 11 critical core configurations of the HTR-PROTEUS pebble bed experimental program. Various additional reactor physics measurements were performed as part of this program; currently only a total of 37 absorber rod worth measurements have been evaluated as acceptable benchmark experiments for Cores 4, 9, and 10. Dominant uncertainties in the experimental keff for all core configurations come from uncertainties in the ˛ł?U enrichment of the fuel, impurities in the moderator pebbles, and the density and impurity content of the radial reflector. Calculations of keff with MCNP5 and ENDF/B-VII.0 neutron nuclear data are greater than the benchmark values but within 1% and also within the 3? uncertainty, except for Core 4, which is the only randomly packed pebble configuration. Repeated calculations of keff with MCNP6.1 and ENDF/B-VII.1 are lower than the benchmark values and within 1% (~3?) except for Cores 5 and 9, which calculate lower than the benchmark eigenvalues within 4?. The primary difference between the two nuclear data libraries is the adjustment of the absorption cross section of graphite. Simulations of the absorber rod worth measurements are within 3? of the benchmark experiment values. The complete benchmark evaluation details are available in the 2014 edition of the International Handbook of Evaluated Reactor Physics Benchmark Experiments.

  13. Pseudomonas screening assay

    NASA Technical Reports Server (NTRS)

    Margalit, Ruth (inventor)

    1993-01-01

    A method for the detection of Pseudomonas bacteria is described where an Azurin-specific antibody is employed for detecting the presence of Azurin in a test sample. The detection of the presence of Azurin in the sample is a conclusive indicator of the presence of the Pseudomonas bacteria since the Azurin protein is a specific marker for this bacterial strain.

  14. Pseudomonas kuykendallii sp. nov.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This is a submission to the list of microorganisms with standing in nomenclature maintained by the International Journal of Systematic and Evolutionary Microbiology. We wish to have Pseudomonas kuykendallii sp. nov. added to the list as a valid species belonging to the genus Pseudomonas. Three str...

  15. Recombineering Pseudomonas syringae

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Here we report the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecE...

  16. Radiographic manifestations of the temporomandibular joint in a case of Proteus syndrome

    PubMed Central

    Yilmaz, E; Kansu, Ö; Özgen, B; Akçiçek, G; Kansu, H

    2013-01-01

    Proteus syndrome is a rare disorder with progressive asymmetrical and disproportionate overgrowth of various tissues of the body. The syndrome is characterized by a wide range of malformations, including craniofacial deformities. Extraoral examination revealed several of the classical craniofacial features of Proteus syndrome: pronounced hemifacial hypertrophy, macrodactyly and hyperostosis. Intraoral examination revealed a high arched palate and gingival hyperplasia. Other findings were unilateral enlargement of the tongue, alveolar growth and dilaceration of the roots of the teeth. There were severe degenerative changes and deformities in the left temporomandibular joint but the oversized condyle was asymptomatic; there was no pain, limitation and deviation at mouth opening. Treatment was not necessary owing to the asymptomatic situation but periodic follow-up with clinical and radiographic examination was considered. The aim of this article is to describe the radiographic manifestations of an asymptomatic condyle malformation and other craniofacial, oral and dental findings in a 33-year-old female patient with known Proteus syndrome. PMID:22241876

  17. Radiographic manifestations of the temporomandibular joint in a case of Proteus syndrome.

    PubMed

    Yilmaz, E; Kansu, O; Ozgen, B; Akçiçek, G; Kansu, H

    2013-01-01

    Proteus syndrome is a rare disorder with progressive asymmetrical and disproportionate overgrowth of various tissues of the body. The syndrome is characterized by a wide range of malformations, including craniofacial deformities. Extraoral examination revealed several of the classical craniofacial features of Proteus syndrome: pronounced hemifacial hypertrophy, macrodactyly and hyperostosis. Intraoral examination revealed a high arched palate and gingival hyperplasia. Other findings were unilateral enlargement of the tongue, alveolar growth and dilaceration of the roots of the teeth. There were severe degenerative changes and deformities in the left temporomandibular joint but the oversized condyle was asymptomatic; there was no pain, limitation and deviation at mouth opening. Treatment was not necessary owing to the asymptomatic situation but periodic follow-up with clinical and radiographic examination was considered. The aim of this article is to describe the radiographic manifestations of an asymptomatic condyle malformation and other craniofacial, oral and dental findings in a 33-year-old female patient with known Proteus syndrome. PMID:22241876

  18. Repression of AKT signaling by ARQ 092 in cells and tissues from patients with Proteus syndrome

    PubMed Central

    Lindhurst, Marjorie J.; Yourick, Miranda R.; Yu, Yi; Savage, Ronald E.; Ferrari, Dora; Biesecker, Leslie G.

    2015-01-01

    A somatic activating mutation in AKT1, c.49G>A, pGlu17Lys, that results in elevated AKT signaling in mutation-positive cells, is responsible for the mosaic overgrowth condition, Proteus syndrome. ARQ 092 is an allosteric pan-AKT inhibitor under development for treatment in cancer. We tested the efficacy of this drug for suppressing AKT signaling in cells and tissues from patients with Proteus syndrome. ARQ 092 reduced phosphorylation of AKT and downstream targets of AKT in a concentration-dependent manner in as little as two hours. While AKT signaling was suppressed with ARQ 092 treatment, cells retained their ability to respond to growth factor stimulation by increasing pAKT levels proportionally to untreated cells. At concentrations sufficient to decrease AKT signaling, little reduction in cell viability was seen. These results indicate that ARQ 092 can suppress AKT signaling and warrants further development as a therapeutic option for patients with Proteus syndrome. PMID:26657992

  19. Welwitschia mirabilis 

    E-print Network

    Leon Pratorius

    2011-08-10

    coping systems to environmental stress. In natural environments, males of many species with more symmetrical features have higher reproductive success as measured by the number of offspring. (Koshi et al., 2007; Gallup, Frederick, & Pipitone, 2008.... Specifically, preferences for more dominant males should be stronger during cycles that are more fertile than cycles that are less fertile. The theory postulates that given ancestral environments, some cycles were more fertile than others, and that estrogen...

  20. Aetas Mirabilis

    NASA Astrophysics Data System (ADS)

    Swartz, Clifford E.

    1999-04-01

    I recently had occasion to read again Galileo's Dialogue on Two New Sciences. Although it's hard to read Newton's Principia with its geometrical proofs, it's a delight to follow the banter and brilliant reasoning of the Dialogues. What a marvelous time to have been a scientist! Only three hundred and sixty-five years ago our whole world view was being overthrown by a few men, armed with their brains and some new optical devices.

  1. A minimal form of Proteus syndrome presenting with macrodactyly and hand hyperplasia.

    PubMed

    Morelli, Federica; Feliciani, Claudio; Toto, Paola; De Benedetto, Anna; Tulli, Antonello

    2003-01-01

    Proteus syndrome is a rare congenital disorder characterized by progressive course and great variability of clinical presentation with partial gigantism of extremities, hemihyperplasia with macrocephaly, epidermal nevus, mesodermal hamartomas and the presence of peculiar cerebriform masses on the palms/soles. Many atypical cases have been reported and this is probably due to the mosaicism of the genetic disorder displaying different clinical features. We describe a patient with an extremely mild form of Proteus syndrome presenting macrodactyly and hyperplasia of one hand which was misdiagnosed until the age of 33 years. PMID:12695139

  2. Analysis of the thorium axial blanket experiments in the PROTEUS reactor

    SciTech Connect

    White, J.R.; Ingersoll, D.T.; Schmocker, U.

    1980-01-01

    An extensive program of reactor physics experiments in GCFR fuel pin lattices has been completed recently at the PROTEUS critical facility located at EIR laboratory in Switzerland. The PROTEUS reactor consists of a central test zone surrounded by a uranium buffer and thermal driver region. The test lattices included a PuO/sub 2//UO/sub 2/ fuel region with internal and axial blankets of UO/sub 2/, ThO/sub 2/, and thorium metal. Detailed analysis of the thorium-bearing lattices has been performed at EIR and at ORNL in order to validate nuclear data and methods used for reactor physics analysis of advanced GCFR designs.

  3. Efficacy of some colloidal silver preparations and silver salts against Proteus bacteria, one possible cause of rheumatoid arthritis.

    PubMed

    Disaanayake, D M B T; Faoagali, Joan; Laroo, Hans; Hancock, Gerald; Whitehouse, Michael

    2014-04-01

    There has been increased interest in the role of anti-Proteus antibodies in the aetiology of rheumatoid arthritis (RA) and whether chemotherapeutic agents active against Proteus species might reduce the risk and/or exacerbations of RA. We examined the in vitro antibacterial effects of ten different silver preparations which were either ionic silver [Ag(I)] solutions or nanoparticulate silver (NPS) (Ag(0)) suspensions against ATCC and two wild (clinical) strains of Proteus. The data establish the low minimum inhibitory concentration and minimum bactericidal concentration of all the silver formulations tested against these four Proteus strains. In a pilot study, a potent NPS preparation ex vivo showed long-lasting anti-Proteus activity in a normal human volunteer. PMID:24390313

  4. Pseudomonas folliculitis in arabian baths

    E-print Network

    Molina-Leyva, Alejandro; Ruiz-Ruigomez, Maria

    2013-01-01

    infection in our case, satisfy these characteristic conditions for Pseudomonas aeruginosaPseudomonas aeruginosa was isolated from a pustule culture. Pseudomonas folliculitis is a bacterial infectionPseudomonas aeruginosa. Typically, the utility of blood cultures is poor even when fever is present because these infections

  5. Two new naphthalene glucosides and other bioactive compounds from the carnivorous plant Nepenthes mirabilis.

    PubMed

    Thanh, Nguyen Van; Thao, Nguyen Phuong; Dat, Le Duc; Huong, Phan Thi Thanh; Lee, Sang Hyun; Jang, Hae Dong; Cuong, Nguyen Xuan; Nam, Nguyen Hoai; Kiem, Phan Van; Minh, Chau Van; Kim, Young Ho

    2015-10-01

    Two new naphthalene diglucosides named nepenthosides A (1) and B (2), together with eleven known compounds (3-13), were isolated from the carnivorous plant Nepenthes mirabilis. The structures of these compounds were elucidated based on extensive spectroscopic analysis, including 1D- and 2D-NMR, and MS. The antioxidant activities of compounds 1-13 were evaluated in terms of their peroxyl radical-scavenging (trolox equivalent, TE) and reducing capacities. All isolates showed peroxyl radical-scavenging and reducing activities at concentrations of 1.0 and 10.0 ?M. Anti-osteoporotic activities were investigated using murine osteoclastic RAW 264.7 cells. Compounds 1-7 and 9-12 significantly suppressed tartrate-resistant acid phosphatase activity down to 91.13 ± 1.18 to 42.39 ± 1.11 %, relative to the control (100 %) in nuclear factor-?B ligand (RAN?L)-induced osteoclastic RAW 264.7 macrophage cells. PMID:25724283

  6. Mathemimetics II. Demonstratio Mirabilis of FLT by infinitely ascending cubical crystal growth

    NASA Astrophysics Data System (ADS)

    Trell, Erik

    2012-09-01

    Emulating Nature by observation and ground-up application of its patterns, structures and processes is a classical scientific practice which under the designation of Biomimetics has now been brought to the Nanotechnology scale where even highly complex systems can be realized by continuous or cyclically reiterated assembly of the respective self-similar eigen-elements, modules and algorithms right from their infinitesimal origin. This is actually quite akin to the genuine mathematical art and can find valuable renewed use as here exemplified by the tentatively original Demonstratio Mirabilis of FLT (Fermat's Last Theorem, or, in that case, Triumph) by infinitely ascending sheet-wise cubical crystal growth leading to the binomial `magic triangle' of his close fellow Blaise Pascal.

  7. Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fish Gillichthys mirabilis.

    PubMed

    Jayasundara, Nishad; Tomanek, Lars; Dowd, W Wesley; Somero, George N

    2015-05-01

    Cardiac function is thought to play a central role in determining thermal optima and tolerance limits in teleost fishes. Investigating proteomic responses to temperature in cardiac tissues may provide insights into mechanisms supporting the thermal plasticity of cardiac function. Here, we utilized a global proteomic analysis to investigate changes in cardiac protein abundance in response to temperature acclimation (transfer from 13°C to 9, 19 and 26°C) in a eurythermal goby, Gillichthys mirabilis. Proteomic data revealed 122 differentially expressed proteins across acclimation groups, 37 of which were identified using tandem mass-spectrometry. These 37 proteins are involved in energy metabolism, mitochondrial regulation, iron homeostasis, cytoprotection against hypoxia, and cytoskeletal organization. Compared with the 9 and 26°C groups, proteins involved in energy metabolism increased in 19°C-acclimated fish, indicating an overall increase in the capacity for ATP production. Creatine kinase abundance increased in 9°C-acclimated fish, suggesting an important role for the phosphocreatine energy shuttle in cold-acclimated hearts. Both 9 and 26°C fish also increased abundance of hexosaminidase, a protein directly involved in post-hypoxia stress cytoprotection of cardiac tissues. Cytoskeletal restructuring appears to occur in all acclimation groups; however, the most prominent effect was detected in 26°C-acclimated fish, which exhibited significantly increased actin levels. Overall, proteomic analysis of cardiac tissue suggests that the capacity to adjust ATP-generating processes is crucial to the thermal plasticity of cardiac function. Furthermore, G. mirabilis may optimize cellular functions at temperatures near 19°C, which lies within the species' preferred temperature range. PMID:25954043

  8. [Initial experience with prophylactic Proteus vaccine immunization in an emergency clinic].

    PubMed

    Zdanovskaia, L K; Kliukvin, I Iu; Bulava, G V; Kre?nin, L S; Levina, L A; Kaverina, K G

    1990-11-01

    As early as the first 24 hours since a severe isolated locomotor trauma the patients were immunized with Proteus vaccine. Such vaccination is shown to stimulate the production of serum specific antibodies, to reduce contamination of the wound with gram-negative agents, to shorten hospital stay and healing of the wound versus subjects vaccinated with staphylococcal anatoxin and nonimmunized patients. PMID:2283824

  9. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Proteus spp. (Weil-Felix) serological reagents. 866.3410 Section 866.3410 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents §...

  10. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Proteus spp. (Weil-Felix) serological reagents. 866.3410 Section 866.3410 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents §...

  11. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Proteus spp. (Weil-Felix) serological reagents. 866.3410 Section 866.3410 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents §...

  12. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Proteus spp. (Weil-Felix) serological reagents. 866.3410 Section 866.3410 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents §...

  13. 21 CFR 866.3410 - Proteus spp. (Weil-Felix) serological reagents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Proteus spp. (Weil-Felix) serological reagents. 866.3410 Section 866.3410 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents §...

  14. Endometrioid Paraovarian Borderline Cystic Tumor in an Infant with Proteus Syndrome

    PubMed Central

    Vasquez, Liliana; Tello, Mariela; Maza, Ivan; Oscanoa, Monica; Dueńas, Milagros; Castro, Haydee; Latorre, Alan

    2015-01-01

    Ovarian and paraovarian neoplasms are uncommon in children, mainly originating from germ cell tumors and, least frequently, epithelial tumors. There is an association between genital tract tumors and Proteus syndrome, a rare, sporadic, and progressive entity, characterized by a postnatal overgrowth in several tissues caused by a mosaic mutation in the AKT1 gene. We describe a 20-month-old asymptomatic infant with Proteus syndrome who developed an endometrioid paraovarian borderline cystic tumor. This is the youngest patient so far reported in the literature with this rare syndrome and an adnexal tumor of borderline malignancy. A total of nine patients have been described with female tract tumors and associated Proteus syndrome, which includes bilateral ovarian cystadenomas and other benign masses. A paraovarian neoplasm is extremely rare in children and could be considered a criterion for Proteus syndrome. Standardized staging and treatment of these tumors are not well established; however, most authors conclude that these neoplasms must be treated as their ovarian counterparts. PMID:26558123

  15. Endometrioid Paraovarian Borderline Cystic Tumor in an Infant with Proteus Syndrome.

    PubMed

    Vasquez, Liliana; Tello, Mariela; Maza, Ivan; Oscanoa, Monica; Dueńas, Milagros; Castro, Haydee; Latorre, Alan

    2015-01-01

    Ovarian and paraovarian neoplasms are uncommon in children, mainly originating from germ cell tumors and, least frequently, epithelial tumors. There is an association between genital tract tumors and Proteus syndrome, a rare, sporadic, and progressive entity, characterized by a postnatal overgrowth in several tissues caused by a mosaic mutation in the AKT1 gene. We describe a 20-month-old asymptomatic infant with Proteus syndrome who developed an endometrioid paraovarian borderline cystic tumor. This is the youngest patient so far reported in the literature with this rare syndrome and an adnexal tumor of borderline malignancy. A total of nine patients have been described with female tract tumors and associated Proteus syndrome, which includes bilateral ovarian cystadenomas and other benign masses. A paraovarian neoplasm is extremely rare in children and could be considered a criterion for Proteus syndrome. Standardized staging and treatment of these tumors are not well established; however, most authors conclude that these neoplasms must be treated as their ovarian counterparts. PMID:26558123

  16. 21 CFR 522.88 - Sterile amoxicillin trihydrate for suspension.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary infections (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal infections (bacterial gastroenteritis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis due to...

  17. 21 CFR 522.88 - Sterile amoxicillin trihydrate for suspension.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary infections (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal infections (bacterial gastroenteritis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis due to...

  18. 21 CFR 522.88 - Sterile amoxicillin trihydrate for suspension.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary infections (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal infections (bacterial gastroenteritis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis due to...

  19. 21 CFR 522.88 - Sterile amoxicillin trihydrate for suspension.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary infections (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal infections (bacterial gastroenteritis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis due to...

  20. Indicator For Pseudomonas Bacteria

    NASA Technical Reports Server (NTRS)

    Margalit, Ruth

    1990-01-01

    Characteristic protein extracted and detected. Natural protein marker found in Pseudomonas bacteria. Azurin, protein containing copper readily extracted, purified, and used to prepare antibodies. Possible to develop simple, fast, and accurate test for marker carried out in doctor's office.

  1. 40 CFR 180.1114 - Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS...

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS; exemptions from the requirement of a tolerance... Tolerances § 180.1114 Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas...

  2. 40 CFR 180.1114 - Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS...

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS; exemptions from the requirement of a tolerance... Tolerances § 180.1114 Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas...

  3. 40 CFR 180.1114 - Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS...

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS; exemptions from the requirement of a tolerance... Tolerances § 180.1114 Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas...

  4. Hot Tub Rash (Pseudomonas Folliculitis)

    MedlinePLUS

    newsletter | contact Share | Hot Tub Rash ( Pseudomonas Folliculitis) Information for adults A A A This image displays follicular elevations of the skin and small pus-filled lesions. Overview Hot tub rash ( Pseudomonas folliculitis) is an infection of ...

  5. Pseudomonas aeruginosa in Healthcare Settings

    MedlinePLUS

    ... infection? Pseudomonas infection is caused by strains of bacteria found widely in the environment; the most common type causing infections in humans is called Pseudomonas aeruginosa . For more images of this bacterium, search the Public Health Image ...

  6. Chlorella mirabilis as a Potential Species for Biomass Production in Low-Temperature Environment

    PubMed Central

    Shukla, S. P.; Kvíderová, J.; T?íska, J.; Elster, J.

    2013-01-01

    Successful adaptation/acclimatization to low temperatures in micro-algae is usually connected with production of specific biotechnologically important compounds. In this study, we evaluated the growth characteristics in a micro-scale mass cultivation of the Antarctic soil green alga Chlorella mirabilis under different nitrogen and carbon sources followed by analyses of fatty acid contents. The micro-scale mass cultivation was performed in stable (in-door) and variable (out-door) conditions during winter and/or early spring in the Czech Republic. In the in-door cultivation, the treatments for nitrogen and carbon sources determination included pure Z medium (control, Z), Z medium?+?5% glycerol (ZG), Z medium?+?5% glycerol?+?50??M KNO3 (ZGN), Z medium?+?5% glycerol?+?200??M NH4Cl (ZGA), Z medium?+?5% glycerol?+?1?mM Na2CO3 (ZNC), Z medium?+?5% glycerol?+?1?mM Na2CO3?+?200??M NH4Cl (ZGCA) and Z medium?+?5% glycerol?+?1?mM Na2CO3?+?50??M KNO3 (ZGCN) and were performed at 15°C with an irradiance of 75??mol?m?2?s?1. During the out-door experiments, the night-day temperature ranged from ?6.6 to 17.5°C (daily average 3.1?±?5.3°C) and irradiance ranged from 0 to 2,300??mol?m?2?s?1 (daily average 1,500?±?1,090??mol?m?2?s?1). Only the Z, ZG, ZGN, and ZGC treatments were used in the out-door cultivation. In the in-door mass cultivation, all nitrogen and carbon sources additions increased the growth rate with the exception of ZGA. When individual sources were considered, only the effect of 5% glycerol addition was significant. On the other hand, the growth rate decreased in the ZG and ZGN treatments in the out-door experiment, probably due to carbon limitation. Fatty acid composition showed increased production of linoleic acid in the glycerol treatments. The studied strain of C. mirabilis is proposed to be a promising source of linoleic acid in low-temperature-mass cultivation biotechnology. This strain is a perspective model organism for biotechnology in low-temperature conditions. PMID:23630521

  7. Polymicrobial Ventriculitis Involving Pseudomonas fulva

    PubMed Central

    Rebolledo, Paulina A.; Vu, Catphuong Cathy L.; Carlson, Renee Donahue; Kraft, Colleen S.; Anderson, Evan J.

    2014-01-01

    Infections due to Pseudomonas fulva remain a rare but emerging concern. A case of ventriculitis due to Enterobacter cloacae and Pseudomonas fulva following placement of an external ventricular drain is described. Similar to other reports, the organism was initially misidentified as Pseudomonas putida. The infection was successfully treated with levofloxacin. PMID:24648556

  8. Operative Management of OSAS in a Complex Case of Proteus Syndrome

    PubMed Central

    Cantone, Elena; Cavaliere, Michele; Castagna, Giovanni; Marino, Anna; Del Vecchio, Luigi; Iengo, Maurizio

    2015-01-01

    Obstructive sleep apnea syndrome (OSAS) is a common disorder in childhood with high prevalence in syndromic subjects with craniofacial malformations. Proteus Syndrome (PS) is a rare hamartoneoplastic disorder associated with disproportionate and asymmetric overgrowth of body parts and hypertrophy or malformation of lymphatic tissues, such as palatine tonsils. We report a case of a 12-year-old boy diagnosed with Proteus Syndrome (PS) and suffering from OSAS due to asymmetric palatine tonsillar hypertrophy, treated with partial resection of left tonsil. To avoid the risk of a general anesthesia and remove only the obstructive portion of the palatine tonsil bipolar radiofrequency-induced thermotherapy (RFITT) under local anesthesia was performed. Recovery of the obstructive respiratory disease was obtained. To our knowledge, this is the first case reported in the literature of partial tonsillar resection performed in a patient with PS suffering from OSAS under local anesthesia. PMID:26199778

  9. Empyema Necessitans Complicating Pleural Effusion Associated with Proteus Species Infection: A Diagnostic Dilemma

    PubMed Central

    Yauba, M. S.; Ahmed, H.; Imoudu, I. A.; Yusuf, M. O.; Makarfi, H. U.

    2015-01-01

    Background. Empyema necessitans, a rare complication of pleural effusion, could result in significant morbidity and mortality in children. It is characterized by the dissection of pus through the soft tissues and the skin of the chest wall. Mycobacterium tuberculosis and Actinomyces israelii are common causes but Gram negative bacilli could be a rare cause. However, there were challenges in differentiating between Mycobacterium tuberculosis and nontuberculous empyema in a resource poor setting like ours. We report a child with pleural effusion and empyema necessitans secondary to Proteus spp. infection. Methods. We describe a 12-year-old child with empyema necessitans complicating pleural effusion and highlight management challenges. Results. This case was treated with quinolones, antituberculous drugs, chest tube drainage, and nutritional rehabilitation. Conclusion. Empyema necessitatis is a rare condition that can be caused by Gram negative bacterial pathogens like Proteus species. PMID:25893125

  10. Acetylcholinesterase inhibitory activity of pigment echinochrome A from sea urchin Scaphechinus mirabilis.

    PubMed

    Lee, Sung Ryul; Pronto, Julius Ryan D; Sarankhuu, Bolor-Erdene; Ko, Kyung Soo; Rhee, Byoung Doo; Kim, Nari; Mishchenko, Natalia P; Fedoreyev, Sergey A; Stonik, Valentin A; Han, Jin

    2014-06-01

    Echinochrome A (EchA) is a dark-red pigment of the polyhydroxynaphthoquinone class isolated from sea urchin Scaphechinus mirabilis. Acetylcholinesterase (AChE) inhibitors are used in the treatment of various neuromuscular disorders, and are considered as strong therapeutic agents for the treatment of Alzheimer's disease (AD). Although EchA is clinically used to treat ophthalmic diseases and limit infarct formation during ischemia/ reperfusion injury, anti-AChE effect of EchA is still unknown. In this study, we investigated the anti-AChE effect of EchA in vitro. EchA and its exhausted form which lost anti-oxidant capacity did not show any significant cytotoxicy on the H9c2 and A7r5 cells. EchA inhibited AChE with an irreversible and uncompetitive mode. In addition, EchA showed reactive oxygen species scavenging activity, particularly with nitric oxide. These findings indicate new therapeutic potential for EchA in treating reduced acetylcholine-related diseases including AD and provide an insight into developing new AChE inhibitors. PMID:24918454

  11. Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A from Sea Urchin Scaphechinus mirabilis

    PubMed Central

    Lee, Sung Ryul; Pronto, Julius Ryan D.; Sarankhuu, Bolor-Erdene; Ko, Kyung Soo; Rhee, Byoung Doo; Kim, Nari; Mishchenko, Natalia P.; Fedoreyev, Sergey A.; Stonik, Valentin A.; Han, Jin

    2014-01-01

    Echinochrome A (EchA) is a dark-red pigment of the polyhydroxynaphthoquinone class isolated from sea urchin Scaphechinus mirabilis. Acetylcholinesterase (AChE) inhibitors are used in the treatment of various neuromuscular disorders, and are considered as strong therapeutic agents for the treatment of Alzheimer’s disease (AD). Although EchA is clinically used to treat ophthalmic diseases and limit infarct formation during ischemia/reperfusion injury, anti-AChE effect of EchA is still unknown. In this study, we investigated the anti-AChE effect of EchA in vitro. EchA and its exhausted form which lost anti-oxidant capacity did not show any significant cytotoxicy on the H9c2 and A7r5 cells. EchA inhibited AChE with an irreversible and uncompetitive mode. In addition, EchA showed reactive oxygen species scavenging activity, particularly with nitric oxide. These findings indicate new therapeutic potential for EchA in treating reduced acetylcholine-related diseases including AD and provide an insight into developing new AChE inhibitors. PMID:24918454

  12. Proteus syndrome: a case report and a case study review in China

    PubMed Central

    Zhang, Xi-Bao; Li, Chang-Xing; He, Yu-Qing; Zhang, San-Quan; Cai, Yan-Xia

    2010-01-01

    Proteus syndrome (PS) is a rare and sporadic disorder characterized by overgrowth of multiple tissues and a propensity to develop particular neoplasms. The clinical manifestations of PS include macrodactyly, vertebral abnormalities, asymmetric limb overgrowth and length discrepancy, hyperostosis, abnormal and asymmetric fat distribution, asymmetric muscle development, connective tissue nevi, and vascular malformations. We report a 16-year old female patient who manifested a number of these complications and review the Chinese literature about the diagnosis, natural history, and management of PS. PMID:25386239

  13. Growth responses and cadmium accumulation of Mirabilis jalapa L. under interaction between cadmium and phosphorus.

    PubMed

    Yu, Zhiguo; Zhou, Qixing

    2009-08-15

    Based on the identification of Mirabilis jalapa L. as a new Cd-hyperaccumulating ornamental, the growth response of the plant to interaction between cadmium (Cd) and phosphorus (P), its effect on the Cd accumulation in the species and relevant mechanisms were further investigated by the pot-culture experiment with chemical analyses and the X-ray absorption near edge structure spectra (XANES). It showed that the leaf, shoot and root biomass (as dry matter) increased with an increase in P supplies from 20 to 100 mg kg(-1) at various tested Cd levels except 10 mg kg(-1). The Cd accumulation in the leaves and shoots significantly decreased with increasing P concentrations from 20 to 500 mg kg(-1) at the Cd concentrations from 10 to 50 mg kg(-1), except the Cd level at 100 mg kg(-1). It was also found that the translocation factor of Cd in M. jalapa L. reached the maximum at different tested Cd levels when the concentration of added P was 100 mg kg(-1), but the bioaccumulation factor of Cd decreased with increasing P. This changing law may be responsible for the mechanism of P immobilizing Cd. The investigation using the P-K edge XANES showed the spectra of adsorbed phosphate in the shoots exhibited a stronger white-line peak than that in the leaves and roots, and the oscillation near 2165eV was more intense. Besides, the P-K edge XANES spectra for M. jalapa L. indicated P may exist as Cd-phosphate. Thus, it can be inferred that the addition of P at appropriate contents may be a useful approach to enhance the plant growth and to immobilize Cd in the Cd-contaminated soil. Furthermore, P and Cd may form a deposit in plants to tolerate Cd toxicity for reducing the degree of the structural damage of the plant. PMID:19179005

  14. [Change in the quantity of coliform and Proteus bacteria during the ripening of raw, nonperishable meat products].

    PubMed

    Iordanov, I; Delchev, Kh

    1977-01-01

    Studied were the changes in the amount of coliform and Proteus bacteria in the production of raw-dried and raw-smoked unperishable meat products, employing model testing procedures of experimentally contaminated meat products with Escherichia coli and Bacterium proteus, through following up the changes in the counts of coliform and Proteus bacteria in batches of regular production, and by the investigation of raw meat samples of varying moisture content. The studies were carried out at eight enterprises working under different technologic conditions. A total of twenty series of model experiments were carried out with artificially contaminated batches of products as well as twenty-one series of experiments with batches of regular production, and some 93 meat products, dried to a various extent, were sampled. Results showed that during the ripening process with unperishable meat products the reduce in the numbers of coliform bacteria is more intense than the decrease in the Proteus organisms. The process of smoking did not change the amount of these microbes. In unperishable raw meat products that meet the requirements of the Bulgarian State Standard coliforms are found in 43 per cent of the samples, and Proteus bacteria are not present. PMID:343357

  15. Preliminary Analysis of the Transient Reactor Test Facility (TREAT) with PROTEUS

    SciTech Connect

    Connaway, H. M.; Lee, C. H.

    2015-11-30

    The neutron transport code PROTEUS has been used to perform preliminary simulations of the Transient Reactor Test Facility (TREAT). TREAT is an experimental reactor designed for the testing of nuclear fuels and other materials under transient conditions. It operated from 1959 to 1994, when it was placed on non-operational standby. The restart of TREAT to support the U.S. Department of Energy’s resumption of transient testing is currently underway. Both single assembly and assembly-homogenized full core models have been evaluated. Simulations were performed using a historic set of WIMS-ANL-generated cross-sections as well as a new set of Serpent-generated cross-sections. To support this work, further analyses were also performed using additional codes in order to investigate particular aspects of TREAT modeling. DIF3D and the Monte-Carlo codes MCNP and Serpent were utilized in these studies. MCNP and Serpent were used to evaluate the effect of geometry homogenization on the simulation results and to support code-to-code comparisons. New meshes for the PROTEUS simulations were created using the CUBIT toolkit, with additional meshes generated via conversion of selected DIF3D models to support code-to-code verifications. All current analyses have focused on code-to-code verifications, with additional verification and validation studies planned. The analysis of TREAT with PROTEUS-SN is an ongoing project. This report documents the studies that have been performed thus far, and highlights key challenges to address in future work.

  16. Is the blind cave salamander Proteus anguinus equiped for magnetic orientation ?

    NASA Astrophysics Data System (ADS)

    Bouquerel, H.; Valet, J. P.

    2003-04-01

    The Proteus anguinus is a blind cave salamander which can develop the ability of using the earth’s magnetic field for orientation and navigation. It has been shown that the strength of the geomagnetic field is not strong enough to excite the electroreceptors of these animals through induction mechanism so that the most likely hypothesis is that they would use cristals of magnetite as permanent magnets. We have been looking for evidence of remanent magnetism in several proteus collected from the underground CNRS laboratory at Moulis (France). Because the level of natural remanent magnetization, if any, was too low to be measured with confidence using a 3 axis squid 2G magnetometer (even bringing the animals as close as possible to the sensors), we stepwise remagnetized the samples between 0.2 and 1.2T. Measurements were performed in different parts of three proteus bodies. No significant magnetization was detected in the head, most of the signal being concentrated in the lower body of the animal. Saturation was attained after 0.2T while stepwise demagnetization by alternating field showed that most magnetization was removed after 40 mT (medium destructive field, MDF of about 10 mT), which is typical of magnetite. Independent measurements of clay soils taken from the surrounding immediate environment of the animals reveal a different magnetic signature for saturation, MDF and viscosity. Thus there is no apparent and direct link between food absorbed from their environment and the magnetic remamence of the animals. New experiments are currently in progress to determine whether magnetite is the unique magnetic carrier and also to provide better clue about the magnetic granulometry and its distribution.

  17. Effect of Coliform and Proteus Bacteria on Growth of Staphylococcus aureus1

    PubMed Central

    DiGiacinto, J. V.; Frazier, W. C.

    1966-01-01

    Cultures of coliform and Proteus bacteria, mostly from foods, were tested for their effect on growth of Staphylococcus aureus in Trypticase Soy Broth. Inhibition of the staphylococcus by these competitors increased with increasing proportions of inhibiting (effector) bacteria in the inoculum and decreasing incubation temperatures (37 to 10 C). Time required for 2 × 104 staphylococci to increase to 5 × 106 cells per milliliter, the minimal number assumed to be necessary for food poisoning, varied with the species of effector, the original ratio of effector bacteria to staphylococci in the medium, and the incubation temperature. When the original ratio was 100:1, the staphylococci did not reach 5 × 106 cells per milliliter at 10, 15, 22, or 30 C (with one exception), when growing with cultures representing six species of coliform bacteria and two of Proteus. When the ratio was 1:1, all effectors either greatly delayed the staphylococcus or prevented it from reaching hazardous numbers at 15 C, six of the eight caused a delay of 2 to 3 hr at 22 C, and only Escherichia coli delayed the coccus at 30 C. All effectors were ineffective at 22 and 30 C when original numbers of effectors and staphylococci were in the ratio 1:100. Greatest overall inhibition was by E. coli, E. freundii, and Proteus vulgaris, and these species were more effective than the others at 22 and 30 C. Aerobacter cloacae and Paracolobactrum aerogenoides were more effective at 15 C. In general, results were similar with different strains of a species. Except for Aerobacter aerogenes, Klebsiella sp., and P. aerogenoides, which apparently did not compete for nutrients, inhibition of the staphylococcus was by a combination of antibiotic substances and competition for nutrients. PMID:5330677

  18. Proteus - A Mission to Investigate the Origins of Earth’s Water

    NASA Astrophysics Data System (ADS)

    Meech, Karen J.; Castillo-Rogez, Julie Claire

    2015-08-01

    We still do not know how water and ingredients necessary for life were delivered to our planet. Comets were long thought to have seeded Earth with water, but new models and measurements have shown that comets may not be the right place to look. A growing number of small volatile-rich worlds in the outer asteroid belt - the main belt comets (MBCs) - have been observed to shed dust tails near perihelion and may hold the key to understanding the origin of inner solar system water.Proteus is a proposed Discovery-class solar system origins mission. Proteus, a 6.5-year mission, launches in 2021 to rendezvous with MBC 238P/Read shortly before it reaches perihelion in early 2028 and remains there for five months during its period of maximum activity. 238P/Read is of special interest because en route we have the opportunity to fly past and characterize its likely parent, asteroid 24 Themis. Proteus addresses five science objectives: (1) to determine where Read’s ices formed; (2) to distinguish whether the ices have a nitrogen isotope signature more like Earth or the outer solar system; (3) to determine at what temperature the ices formed; (4) to determine Read’s physical properties using surface composition and geomorphology, and (5) to determine whether Read’s outgassing emanates from discrete sources or diffuse regions and measure the scattering properties of the outgassed dust.To answer questions about the origin of water, isotopic measurements of MBC volatiles will be made with a new sensitive, precise, highly mature mass spectrometer, measuring isotopic abundances of hydrogen, oxygen and nitrogen as well as the abundances of noble gases. These measurements will be correlated with predictions from two types of models: chemical models which describe the distance-dependent chemistry in the nebula and dynamical models which describe where small bodies were gravitationally scattered during the era of giant-planet migration. Proteus additionally flies two redundant Dawn-like cameras, which will be used to map the surface to characterize Read’s shape, rotation, topography, color, geology and jets and combined with radio science to infer its density.

  19. Calcium and initial surface binding phase of pinocytosis in Amoeba proteus

    SciTech Connect

    Prusch, R.D.

    1986-08-01

    The uptake of membrane-bound solute and external medium by bulk-phase pinocytosis in Amoeba proteus is influenced by the level of Ca/sup 2 +/ in the external medium. Increasing external Ca/sup 2 +/ to approx.10/sup -4/ M increases pinocytotic intensity, while increases in Ca/sup 2 +/ above this level decrease the intensity of pinocytosis. The initial interaction of pinocytotic inducers and Ca/sup +2/ at the surface of A moeba proteus was therefore examined. Alcain blue and Na/sup +/, both inducers of pinocytosis, differ in the manner with which they associate with the amoeba surface, suggesting the possibility of different pinocytosis-inducing sites on the amoeba surface. Low levels of external Ca/sup 2 +/ in the range of 3 x 10/sup -5/ to 4.5 x 10/sup -4/ M increase the amount of cationic inducer associated with the cell surface while, at the same time, decreasing anion association with the cell surface. It is suggested that Ca/sup 2 +/ influences ion association with the cell surface by controlling the availability of negative surface sites, which in turn influences pinocytotic intensity. Surface binding of Na/sup +/, Ca/sup 2 +/ and Cl/sup -/ was determined by adding /sup 22/Na, /sup 45/Ca or /sup 36/Cl.

  20. Modeling Multiphase Coastal and Hydraulic Processes in an Interactive Python Environment with the Open Source Proteus Toolkit

    NASA Astrophysics Data System (ADS)

    Kees, C. E.; Farthing, M. W.; Ahmadia, A. J.; Bakhtyar, R.; Miller, C. T.

    2014-12-01

    Hydrology is dominated by multiphase flow processes, due to the importance of capturing water's interaction with soil and air phases. Unfortunately, many different mathematical model formulations are required to model particular processes and scales of interest, and each formulation often requires specialized numerical methods. The Proteus toolkit is a software package for research on models for coastal and hydraulic processes and improvements in numerics, particularly 3D multiphase processes and parallel numerics. The models considered include multiphase flow, shallow water flow, turbulent free surface flow, and various flow-driven processes. We will discuss the objectives of Proteus and recent evolution of the toolkit's design as well as present examples of how it has been used used to construct computational models of multiphase flows for the US Army Corps of Engineers. Proteus is also an open source toolkit authored primarily within the US Army Corps of Engineers, and used, developed, and maintained by a small community of researchers in both theoretical modeling and computational methods research. We will discuss how open source and community development practices have played a role in the creation of Proteus.

  1. Taxonomic characterisation of Proteus terrae sp. nov., a N2O-producing, nitrate-ammonifying soil bacterium.

    PubMed

    Behrendt, Undine; Augustin, Jürgen; Spröer, Cathrin; Gelbrecht, Jörg; Schumann, Peter; Ulrich, Andreas

    2015-12-01

    In the context of studying the influence of N-fertilization on N2 and N2O flux rates in relation to the soil bacterial community composition in fen peat grassland, a group of bacterial strains was isolated that performed dissimilatory nitrate reduction to ammonium and concomitantly produced N2O. The amount of nitrous oxide produced was influenced by the C/N ratio of the medium. The potential to generate nitrous oxide was increased by higher availability of nitrate-N. Phylogenetic analysis based on the 16S rRNA and the rpoB gene sequences demonstrated that the investigated isolates belong to the genus Proteus, showing high similarity with the respective type strains of Proteus vulgaris and Proteus penneri. DNA-DNA hybridization studies revealed differences at the species level. These differences were substantiated by MALDI-TOF MS analysis and several distinct physiological characteristics. On the basis of these results, it was concluded that the soil isolates represent a novel species for which the name Proteus terrae sp. nov. (type strain N5/687(T) =DSM 29910(T) =LMG 28659(T)) is proposed. PMID:26437638

  2. THE ABILITY OF FLAGELLUM-SPECIFIC PROTEUS VULGARIS BACTERIOPHAGE PV22 TO INTERACT WITH CAMPYLOBACTER JEJUNI FLAGELLA IN CULTURE

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Research was initiated to examine Campylobacter jejuni-specific bacteriophage in the Russian Federation to develop alternative control measures for this pathogen. A C. jejuni flagellum-specific phage PV22 from Proteus vulgaris was identified in sewage drainage. This phage interacted with C. jejuni...

  3. Brentuximab vedotin in the treatment of a patient with refractory Hodgkin disease and Proteus syndrome – a case report and discussion

    PubMed Central

    Myakova, Natalia; Smirnova, Nadezhda; Evstratov, Dmitry; Abugova, Yulia; Balashov, Dmitry; Diakonova, Yulia; Konovalov, Dmitry; Skvortsova, Yulia; Maschan, Alexey

    2015-01-01

    Key Clinical Message Treatment of patients with refractory Hodgkin lymphoma is a significant issue. We report a patient with Proteus syndrome and relapsed Hodgkin lymphoma, whose remission was finally achieved after brentuximab vedotin therapy, allowing her to receive a haploidentical stem cell transplant. The possible relationship between both disorders was discussed. PMID:26273462

  4. Characterization and heterologous expression of a PR-1 protein from traps of the carnivorous plant Nepenthes mirabilis.

    PubMed

    Buch, Franziska; Pauchet, Yannick; Rott, Matthias; Mithöfer, Axel

    2014-04-01

    Carnivorous plants capture and digest prey to obtain additional nutrients. Therefore, different trapping mechanisms were developed in different species. Plants of the genus Nepenthes possess pitfall-traps filled with a digestive fluid, which is secreted by the plants themselves. This pitcher fluid is composed of various enzymes to digest the captured prey. Besides hydrolytic enzymes, defense-related proteins have been identified in the fluid. The present study describes the identification and heterologous expression of a pathogenesis-related protein, NmPR-1, from pitchers of Nepenthes mirabilis with features that are unusual for PR-1 proteins. In particular, it was proven to be highly glycosylated and, furthermore, it exhibited antibacterial instead of antifungal activities. These properties are probably due to the specific environment of the pitcher fluid. PMID:24534104

  5. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORES 9 & 10: COLUMNAR HEXAGONAL POINT-ON-POINT PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    SciTech Connect

    John D. Bess

    2013-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  6. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORES 9 & 10: COLUMNAR HEXAGONAL POINT-ON-POINT PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    SciTech Connect

    John D. Bess

    2014-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  7. Operative management of splenic injury in a patient with proteus syndrome

    PubMed Central

    Kannan, Umashankkar; Mishra, Biplab; Subramanian, Arulselvi; Sagar, Sushma; Kumar, Subodh; Singhal, Maneesh

    2014-01-01

    A 20-year-old female with Proteus syndrome sustained splenic injury after fall from a bike. She was initially managed non-operatively at a different hospital for three days and was then referred to our level I trauma center in view of increasing abdominal pain and distention. On admission in the Emergency Department (ED), her pulse rate was 120 per minute and blood pressure was 108/68 mm Hg. Clinical examination showed a distended abdomen with left hypochondrial pain. Ultrasonogram (USG) and Computed Tomography (CT) of the abdomen showed splenomegaly and grade III splenic injury with significant hemoperitoneum. Her hemoglobin was 2.9 g/dl with packed cell volume (PCV) of 12%. In view of low hemoglobin and possibility of pathologic spleen, splenectomy was done. Microscopic examination of the spleen showed hemangiolymphangioma. The patient was discharged on the 5th post-operative day and is doing well at 6 months of follow-up. PMID:25114436

  8. Involvement of myosin VI immunoanalog in pinocytosis and phagocytosis in Amoeba proteus.

    PubMed

    Sobczak, Magdalena; Wasik, Anna; K?opocka, Wanda; Redowicz, Maria Jolanta

    2008-12-01

    Recently, we found a 130-kDa myosin VI immunoanalog in amoeba, which bound to actin in an ATP-sensitive manner and in migrating amoebae colocalized to filamentous actin and dynamin II-containing vesicular structures. To further characterize this protein, we assessed its involvement in amoeba pinocytosis and phagocytosis. Confocal immunofluorescence microscopy and electron microscopy of immunogold-stained cells revealed that, in pinocytotic and phagocytotic amoebae, the myosin VI immunoanalog was visible throughout the cells, including pinocytotic channels and pinocytotic vesicles as well as phagosomes and emerging phagocytic cups. Blocking endogenous protein with anti-porcine myosin VI antibody (introduced into cells by means of microinjection) caused severe defects in pinocytosis and phagocytosis. In comparison with control cells, the treated amoebae formed ~75% less pinocytotic channels and phagocytosed ~65% less Tetrahymena cells. These data indicate that the myosin VI immunoanalog has an important role in pinocytosis and phagocytosis in Amoeba proteus (Pal.). PMID:19088799

  9. Potential pitfalls in volume reduction for the treatment of Proteus syndrome

    PubMed Central

    Tsue, C; Sakamoto, Y; Nakajima, Y; Kishi, K

    2012-01-01

    Proteus syndrome is a rare congenital hamartomatous condition that is characterised by a wide range of malformations. Skin and skeletal developmental malformations are common and may manifest as significant physical anomalies. However, few reports have discussed surgical treatment. A 37-year-old woman presented with disproportionate enlargement of the right buttock, which required volume reduction. The overgrown tissue was combined with vascular malformations, which in our patient were extremely thick. The patient experienced massive blood loss during the procedure, due to which a blood transfusion was performed. Pathological findings revealed partial lipohyperplasia with venous malformations. Thus, during operations including incomplete excision, massive bleeding is always a possibility, but is not commonly described in these instances. PMID:24960753

  10. Experimental evolution of penicillin G acylases from Escherichia coli and Proteus rettgeri.

    PubMed Central

    Daumy, G O; Danley, D; McColl, A S; Apostolakos, D; Vinick, F J

    1985-01-01

    Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms were subjected to environmental conditions which made expression of this enzyme essential for growth, spontaneous mutants were isolated that used different amides as the only source of nitrogen. These mutants acquired the ability to use amides for growth by deregulating the penicillin G acylase and by their evolution to novel substrate specificities. The enzymes expressed by mutants isolated from each genus appeared to have evolved in parallel since each acylase attained similar new substrate specificities when the organisms were subjected to identical selection pressure. Images PMID:3897200

  11. Biology of Pseudomonas stutzeri

    PubMed Central

    Lalucat, Jorge; Bennasar, Antoni; Bosch, Rafael; García-Valdés, Elena; Palleroni, Norberto J.

    2006-01-01

    Pseudomonas stutzeri is a nonfluorescent denitrifying bacterium widely distributed in the environment, and it has also been isolated as an opportunistic pathogen from humans. Over the past 15 years, much progress has been made in elucidating the taxonomy of this diverse taxonomical group, demonstrating the clonality of its populations. The species has received much attention because of its particular metabolic properties: it has been proposed as a model organism for denitrification studies; many strains have natural transformation properties, making it relevant for study of the transfer of genes in the environment; several strains are able to fix dinitrogen; and others participate in the degradation of pollutants or interact with toxic metals. This review considers the history of the discovery, nomenclatural changes, and early studies, together with the relevant biological and ecological properties, of P. stutzeri. PMID:16760312

  12. Advances in understanding Pseudomonas

    PubMed Central

    Wiehlmann, Lutz; Klockgether, Jens; Cramer, Nina

    2014-01-01

    Pseudomonas aeruginosa, the type species of pseudomonads, is an opportunistic pathogen that colonizes a wide range of niches. Current genome sequencing projects are producing previously inconceivable detail about the population biology and evolution of P. aeruginosa. Its pan-genome has a larger genetic repertoire than the human genome, which explains the broad metabolic capabilities of P. aeruginosa and its ubiquitous distribution in aquatic habitats. P. aeruginosa may persist in the airways of individuals with cystic fibrosis for decades. The ongoing whole-genome analyses of serial isolates from cystic fibrosis patients provide the so far singular opportunity to monitor the microevolution of a bacterial pathogen during chronic infection over thousands of generations. Although the evolution in cystic fibrosis lungs is neutral overall, some pathoadaptive mutations are selected during the within-host evolutionary process. Even a single mutation may be sufficient to generate novel complex traits provided that predisposing mutational events have previously occurred in the clonal lineage. PMID:24592321

  13. Cytotoxic, antibacterial and antioxidant activities of extracts of the bark of Melia azedarach (China Berry).

    PubMed

    Zahoor, Muhammad; Ahmed, Manzoor; Naz, Sumaira; Ayaz, Musarrat

    2015-01-01

    Nature provides a variety of drugs and medicinal agents derived from plants. This study was conducted to determine antimicrobial, antioxidant and cytotoxic activities of extracts of Melia azedarach bark with methanol/water (9:1 v/v), chloroform, butanol, hexane, water and ethyl acetate. For the determination of the antimicrobial activities, the agar well diffusion method was employed. Cytotoxicity was studied by brine shrimp lethality assay; antioxidant activities were measured using 1,1-diphenyl-2-picrylhydrazyl. The chloroform extract was active against Enterobacter aerogenes and Proteus mirabilis, the ethyl acetate extract had highest antibacterial spectrum against Pseudomonas aeruginosa, the n-hexane extract had highest inhibition against E. aerogenes, the aqueous extract showed highest activities against P. mirabilis, the butanol fraction showed highest activities against E. aerogenes and the methanolic extract was highly active against P. mirabilis. PMID:25426766

  14. Basal Structure and Attachment of Flagella in Cells of Proteus vulgaris

    PubMed Central

    Abram, Dinah; Koffler, Henry; Vatter, A. E.

    1965-01-01

    Abram, Dinah (Purdue University, Lafayette, Ind.), Henry Koffler, and A. E. Vatter. Basal structure and attachment of flagella in cells of Proteus vulgaris. J. Bacteriol. 90:1337–1354. 1965.—The attachment of flagella to cells of Proteus vulgaris was studied electron microscopically with negatively stained and shadow-cast preparations of ghosts from standard cultures and from special cultures that produced “long forms.” The flagellum, the basal portion of which is hooked, arises within the cell from a nearly spherical structure, 110 to 140 A in diameter. This structure appears to be associated with the cytoplasmic membrane; it may be a part of the membrane or a separate entity that lies just beneath the membrane. Flagella associated with cell walls free from cytoplasmic membrane frequently have larger bodies, 200 to 700 A in diameter, associated with their base. These structures probably consist at least partly of fragments of the cytoplasmic membrane, a portion of which folds around a smaller structure. Flagella in various stages of development were observed in long forms of P. vulgaris cells grown at low temperature. The basal structure of these flagella was similar to that of the long or “mature” flagella. Strands connecting the basal structures were observed in ghosts of long forms; these strands appear to be derived from the cytoplasmic membrane. Flagella were found to be attached to fragments of cell wall and to cytoplasmic membrane in a similar manner as they are attached to ghosts. In isolates of flagella that have been separated from the cells mechanically, the organelles often terminate in hooks which almost always appear naked, but have a different fine structure than the flagellum proper. Images PMID:5848332

  15. 40 CFR 180.1114 - Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS...

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... 40 Protection of Environment 24 2014-07-01 2014-07-01 false Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS; exemptions from the requirement of a tolerance. 180.1114 Section 180.1114 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS...

  16. 40 CFR 180.1114 - Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS...

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... 40 Protection of Environment 25 2012-07-01 2012-07-01 false Pseudomonas fluorescens A506, Pseudomonas fluorescens 1629RS, and Pseudomonas syringae 742RS; exemptions from the requirement of a tolerance. 180.1114 Section 180.1114 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) PESTICIDE PROGRAMS TOLERANCES AND EXEMPTIONS...

  17. 21 CFR 522.90c - Ampicillin sodium.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    .... (including S. equi), Escherichia coli, and Proteus mirabilis, and skin and soft tissue infections (abscesses and wounds) due to Staphylococcus spp., Streptococcus spp., E. coli, and P. mirabilis, when caused...

  18. 21 CFR 522.90c - Ampicillin sodium.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    .... (including S. equi), Escherichia coli, and Proteus mirabilis, and skin and soft tissue infections (abscesses and wounds) due to Staphylococcus spp., Streptococcus spp., E. coli, and P. mirabilis, when caused...

  19. 21 CFR 522.90c - Ampicillin sodium.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    .... (including S. equi), Escherichia coli, and Proteus mirabilis, and skin and soft tissue infections (abscesses and wounds) due to Staphylococcus spp., Streptococcus spp., E. coli, and P. mirabilis, when caused...

  20. 21 CFR 522.90c - Ampicillin sodium.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    .... (including S. equi), Escherichia coli, and Proteus mirabilis, and skin and soft tissue infections (abscesses and wounds) due to Staphylococcus spp., Streptococcus spp., E. coli, and P. mirabilis, when caused...

  1. 21 CFR 522.88 - Amoxicillin.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., tracheobronchitis) due to Staphylococcus aureus, Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary infections (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal infections (bacterial gastroenteritis) due to S. aureus, Streptococcus spp., E. coli, and...

  2. 21 CFR 522.90c - Ampicillin sodium.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    .... (including S. equi), Escherichia coli, and Proteus mirabilis, and skin and soft tissue infections (abscesses and wounds) due to Staphylococcus spp., Streptococcus spp., E. coli, and P. mirabilis, when caused...

  3. Metabolic rates, enzyme activities and chemical compositions of some deep-sea pelagic worms, particularly Nectonemertes mirabilis (Nemertea; Hoplonemertinea) and Poeobius meseres (Annelida; Polychaeta)

    NASA Astrophysics Data System (ADS)

    Thuesen, Erik V.; Childress, James J.

    1993-05-01

    Investigations of metabolic rate, enzyme activity and chemical composition were undertaken on two abundant deep-sea pelagic worms: Nectonemertes mirabilis (Nemertea; Hoplonemertinea) and Poeobius meseres (Annelida; Polychaeta). Six other species of worms ( Pelagonemertes brinkmanni (Nemertea) and the following polychaetes: Pelagobia species A, Tomopteris nisseni, Tomopteris pacifica, Tomopteris species A, and Traviopsis lobifera) were captured in smaller numbers and used for comparison in the physiological and biochemical measurements. Polychaete worms had the highest oxygen consumption rates and, along with N. mirabilis, displayed significant size effects on metabolic rate. Poeobius meseres had the lowest rates of oxygen consumption and displayed no significant relationship of oxygen consumption rate to wet weight. No significant effect of size on the activities of citrate synthase, lactate dehydrogenase or pyruvate kinase was observed in P. meseres or N. mirabilis. Lipid content was higher than protein content for all the worms in this study. Carbohydrate was of little significance in these worms and was usually <0.01% of the total weight. Citrate synthase activities of pelagic worms showed excellent correlation with metabolic rates. It appears that polychaete worms as a group have higher metabolic rates than bathypelagic shrimps, copepods and fishes, and may be the animals with the highest metabolic rates in the bathypelagic regions of the world's oceans.

  4. Verification of the proteus two-dimensional Navier-Stokes code for flat plate and pipe flows

    NASA Technical Reports Server (NTRS)

    Conley, Julianne M.; Zeman, Patrick L.

    1991-01-01

    The Proteus Navier-Stokes Code is evaluated for 2-D/axisymmetric, viscous, incompressible, internal, and external flows. The particular cases to be discussed are laminar and turbulent flows over a flat plate, laminar and turbulent developing pipe flows, and turbulent pipe flow with swirl. Results are compared with exact solutions, empirical correlations, and experimental data. A detailed description of the code set-up, including boundary conditions, initial conditions, grid size, and grid packing is given for each case.

  5. "Hot Tub Rash" and "Swimmer's Ear" (Pseudomonas)

    MedlinePLUS

    Facts About “Hot Tub Rash” and “Swimmer’s Ear” (Pseudomonas) What is Pseudomonas and how can it affect me? Pseudomonas (sue-doh- ... a major cause of infections commonly known as “hot tub rash” and “swimmer’s ear.” This germ is ...

  6. UNCORRECTEDPROOF 1 Structural Characterization of Novel Pseudomonas

    E-print Network

    Burrows, Lori

    UNCORRECTEDPROOF 1 Structural Characterization of Novel Pseudomonas 2 aeruginosa Type IV Pilins 3 Pseudomonas aeruginosa type IV pili, composed of PilA subunits, are used for 19 attachment and twitching Ltd. 42 Edited by I. Wilson 43 Keywords: Pseudomonas aeruginosa; type IV pili; PilA; X

  7. Hypoglycemic and Hypolipidemic Effects of Ethanolic Extract of Mirabilis jalapa L. Root on Normal and Diabetic Mice

    PubMed Central

    Zhou, Ji-Yin; Zhou, Shi-Wen; Zeng, Sheng-Ya; Zhou, Jian-Yun; Jiang, Ming-Jin; He, Yan

    2012-01-01

    The present study investigated the insulin sensitivity, hypoglycemic, and hypolipidemic activities of ethanolic extract of Mirabilis jalapa L. root (EEM) in normal and diabetic mice. After induction of diabetes with streptozotocin, both normal and diabetic mice were singly or repeatedly for 28 days administrated with EEM at doses of 2, 4, 8?g/kg, respectively. Before induction of diabetes, mice were administrated with EEM at doses of 2, 4, 8?g/kg for 14 days and were injected with streptozotocin and continued on EEM administration for another 28 days. Both after and before induction of diabetes, repeated administration with 4, 8?g/kg EEM continually lowered blood glucose level, decreased serum insulin level and improved insulin sensitivity index, and lowered serum total cholesterol, triglyceride levels and triglyceride content in liver and skeletal muscle, and increased glycogen content in these tissues; but repeated administration had no influence on those indexes of normal mice. Single administration with EEM (4, 8?g/kg) showed hypoglycemic effect in oral glucose tolerance test in normal and diabetic mice. Single administration with EEM had no hypoglycemic and hypolipidemic effects on normal and diabetic mice. These results suggest that EEM possesses both potential insulin sensitivity, hypoglycemic, and hypolipidemic effects on diabetes. PMID:22474494

  8. Proteus syndrome: Clinical profile of six patients and review of literature

    PubMed Central

    Angurana, Suresh Kumar; Angurana, Renu Suthar; Panigrahi, Inusha; Marwaha, Ram Kumar

    2013-01-01

    OBJECTIVE: Proteus syndrome (PS) is characterized by patchy or segmental overgrowth and hyperplasia of multiple tissues and organs, along with susceptibility to development of tumors. Very few cases are reported in literature from developing countries. Due to certain overlapping features with other overgrowth syndromes, diagnosis is usually delayed. Our aim was to describe clinical profile of this rare condition in six patients. MATERIALS AND METHODS: Retrospective case sheet review of patients followed in a Pediatric Genetic and Metabolic clinic at a tertiary care institute of North India with a diagnosis of hemihypertrophy/overgrowth syndrome. RESULTS: Six cases presented with asymmetric overgrowth and peculiar features suggestive of PS were included in this study. Age at presentation was 2 months to 10 years; two were males and four were females. Hemihypertrophy was noticed in only one case at birth, and focal overgrowths in rest of other patients were seen later during childhood. CONCLUSION: Due to certain overlapping features with other overgrowth syndromes, diagnosis of PS is usually delayed. Pediatricians are the first persons who come across such patients and they should be aware about this rare condition. PMID:24019623

  9. Thoracolumbar scoliosis in a patient with Proteus syndrome: a case report and literature review.

    PubMed

    Li, Zheng; Shen, Jianxiong; Liang, Jinqian

    2015-02-01

    The Proteus syndrome (PS) is a complex and rare congenital hamartomatous condition with a wide range of malformations. Little is reported about spinal deformity associated with this syndrome. This study presents a case of scoliosis occurring in the setting of PS and explores the possible mechanisms between the 2 diseases. The patient is a 17-year-old Chinese female with scoliosis and hemihypertrophy of the right upper and lower extremity as well as exostosis of the right lower leg joint including the hip, knee, ankle, and toes. These manifestations were suggestive of PS. She underwent a posterior correction at thoracic 2-lumbar 4 (T5-L4) levels, using the Moss-SI spinal system. At 3-month follow-ups, the patient was clinically pain free and well balanced. Plain radiographs showed solid spine fusion with no loss of deformity correction. The severity of scoliosis in PS is progressively aggravated and the correction of the extensive spinal deformities is generally difficult. Therefore, early diagnosis is required for adequate interdisciplinary treatment. PMID:25654373

  10. Typing methods for Proteus rettgeri: comparison of biotype, antibiograms, serotype, and bacteriocin production.

    PubMed

    Anderson, R L; Engley, F B

    1978-12-01

    Two hunderd five strains of Proteus rettgeri from epidemic and nonepidemic sources were differentiated by a new biotyping scheme, agglutination in O antisera, antimicrobial resistance patterns, and a new scheme based on bacteriocin production. The P. rettgeri were divided into 10 groups by their fermentation of lactose, sucrose, D-mannitol, and salicin. These groups were then subdivided into 19 biotypes by other biochemical reactions. Bacteriocin production was tested by the cross-streak method. Thirty-four bacteriocin-sensitive indicator strains were evaluated, and 16 were selected for the final scheme and used to type the 205 P. rettgeri, which were divided into 15 bacteriocin types. Serologically, 43% of the P. rettgeri were O42, 13% were untypable, 4% were O15, and 3% each were O33, O64, and O84 in addition to 31 remaining serotypes. Strains of P. rettgeri from known outbreaks contained fewer biotypes, O groups, and bacteriocin types and were more resistant to antimicrobial agents than endemic strains. Strains with common patterns with all four marker systems were frequently associated with outbreaks. A strong correlation between multiple antibiotic resistance and bacteriocin production was shown. PMID:370146

  11. Burnup calculations and chemical analysis of irradiated fuel samples studied in LWR-PROTEUS phase II

    SciTech Connect

    Grimm, P.; Guenther-Leopold, I.; Berger, H. D.

    2006-07-01

    The isotopic compositions of 5 UO{sub 2} samples irradiated in a Swiss PWR power plant, which were investigated in the LWR-PROTEUS Phase II programme, were calculated using the CASMO-4 and BOXER assembly codes. The burnups of the samples range from 50 to 90 MWd/kg. The results for a large number of actinide and fission product nuclides were compared to those of chemical analyses performed using a combination of chromatographic separation and mass spectrometry. A good agreement of calculated and measured concentrations is found for many of the nuclides investigated with both codes. The concentrations of the Pu isotopes are mostly predicted within {+-}10%, the two codes giving quite different results, except for {sup 242}Pu. Relatively significant deviations are found for some isotopes of Cs and Sm, and large discrepancies are observed for Eu and Gd. The overall quality of the predictions by the two codes is comparable, and the deviations from the experimental data do not generally increase with burnup. (authors)

  12. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Streptococcus spp., Staphylococcus spp., and Escherichia coli; and soft tissue infections (abscesses, wounds, lacerations) due to S. aureus, Streptococcus spp., E. coli, Proteus mirabilis, and Staphylococcus spp....

  13. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Streptococcus spp., Staphylococcus spp., and Escherichia coli; and soft tissue infections (abscesses, wounds, lacerations) due to S. aureus, Streptococcus spp., E. coli, Proteus mirabilis, and Staphylococcus spp....

  14. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Streptococcus spp., Staphylococcus spp., and Escherichia coli; and soft tissue infections (abscesses, wounds, lacerations) due to S. aureus, Streptococcus spp., E. coli, Proteus mirabilis, and Staphylococcus spp....

  15. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Streptococcus spp., Staphylococcus spp., and Escherichia coli; and soft tissue infections (abscesses, wounds, lacerations) due to S. aureus, Streptococcus spp., E. coli, Proteus mirabilis, and Staphylococcus spp....

  16. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Streptococcus spp., Staphylococcus spp., and Escherichia coli; and soft tissue infections (abscesses, wounds, lacerations) due to S. aureus, Streptococcus spp., E. coli, Proteus mirabilis, and Staphylococcus spp....

  17. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli, and Staphylococcus aureus. (iii) Limitations. Administer intramuscularly or subcutaneously. If no improvement is seen...

  18. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...bacterial dermatitis due to Staphylococcus aureus, Streptococcus spp., Staphylococcus spp., and Escherichia...lacerations) due to S. aureus, Streptococcus spp...Proteus mirabilis, and Staphylococcus spp. (iii)...

  19. 21 CFR 520.314 - Cefadroxil.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...due to susceptible strains of Staphylococcus aureus . For the treatment of...Proteus mirabilis , and S. aureus . (ii) Cats ...Pasteurella multocida , S. aureus , Staphylococcus epidermidis , and...

  20. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...bacterial dermatitis due to Staphylococcus aureus, Streptococcus spp., Staphylococcus spp., and Escherichia...lacerations) due to S. aureus, Streptococcus spp...Proteus mirabilis, and Staphylococcus spp. (iii)...

  1. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli, and Staphylococcus aureus. (iii) Limitations. Administer intramuscularly or subcutaneously. If no improvement is seen...

  2. 21 CFR 520.314 - Cefadroxil.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...due to susceptible strains of Staphylococcus aureus . For the treatment of...Proteus mirabilis , and S. aureus . (ii) Cats ...Pasteurella multocida , S. aureus , Staphylococcus epidermidis , and...

  3. 21 CFR 520.314 - Cefadroxil.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...due to susceptible strains of Staphylococcus aureus . For the treatment of...Proteus mirabilis , and S. aureus . (ii) Cats ...Pasteurella multocida , S. aureus , Staphylococcus epidermidis , and...

  4. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli, and Staphylococcus aureus. (iii) Limitations. Administer intramuscularly or subcutaneously. If no improvement is seen...

  5. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...bacterial dermatitis due to Staphylococcus aureus, Streptococcus spp., Staphylococcus spp., and Escherichia...lacerations) due to S. aureus, Streptococcus spp...Proteus mirabilis, and Staphylococcus spp. (iii)...

  6. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...bacterial dermatitis due to Staphylococcus aureus, Streptococcus spp., Staphylococcus spp., and Escherichia...lacerations) due to S. aureus, Streptococcus spp...Proteus mirabilis, and Staphylococcus spp. (iii)...

  7. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli, and Staphylococcus aureus. (iii) Limitations. Administer intramuscularly or subcutaneously. If no improvement is seen...

  8. 21 CFR 520.314 - Cefadroxil.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...due to susceptible strains of Staphylococcus aureus. For the treatment of...Proteus mirabilis , and S. aureus. (ii) Cats. For...Pasteurella multocida , S. aureus , Staphylococcus epidermidis , and...

  9. 21 CFR 520.314 - Cefadroxil.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...due to susceptible strains of Staphylococcus aureus . For the treatment of...Proteus mirabilis , and S. aureus . (ii) Cats ...Pasteurella multocida , S. aureus , Staphylococcus epidermidis , and...

  10. 21 CFR 522.1044 - Gentamicin.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...in the treatment of urinary tract infections (cystitis) caused by Proteus mirabilis, Escherichia coli, and Staphylococcus aureus. (iii) Limitations. Administer intramuscularly or subcutaneously. If no improvement is seen...

  11. 21 CFR 520.88f - Amoxicillin trihydrate tablets.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...bacterial dermatitis due to Staphylococcus aureus, Streptococcus spp., Staphylococcus spp., and Escherichia...lacerations) due to S. aureus, Streptococcus spp...Proteus mirabilis, and Staphylococcus spp. (iii)...

  12. [Antibiotic resistance at a hospital environment. Observations from 1980 to 1986].

    PubMed

    Pistono, P G; Piro, F; Guasco, C; Malcangi, A; Martinetto, P

    1987-01-01

    A total of 1659 bacteria Strains were examined for susceptibility to different drugs by the Kirby-Bauer method. The isolates came from the General Medicine ward of Castellamonte's hospital (USSL 40). The results were retrospectively studied and statistically elaborated. The study lasted from 2 to 7 years depending on the antibiotic considered. The isolates included: Escherichia coli, Klebsiella spp., Serratia spp., Citrobacter spp., Proteus mirabilis, Proteus IND+, Pseudomonas aeruginosa, Pseudomonas spp., Staphylococcus aureus, Staphylococcus spp., Streptococcus faecalis. Penicillines, Cefalosporines, Aminoglycosides, Quinolones, Tetraciclines, Nitrofurantoin, Macrolides, Rifampins, Lincosides and peptide antibiotics were tested. The results of this study can be useful for the choice of the antibiotics in non-specific therapy of infectious diseases and show the frequency of isolation of resistant strains from hospitalized patients. Furthermore it would be important to constitute a regional-epidemiological centre of Infectious Diseases. PMID:3509032

  13. SU-E-T-200: IBA ProteusOne Compact Proton Therapy System Radiation Survey Results

    SciTech Connect

    Zhang, J; Syh, J; Syh, J; White, M; Patel, B; Song, X; Wu, H

    2014-06-01

    Purpose: This study summarizes the results of an initial radiation survey of the Willis-Knighton Cancer Center in Shreveport, Louisiana. The facility houses an IBA ProteusOne compact single room proton therapy unit coupled with a C230 cyclotron that operates at a maximum energy of 230 MeV. Methods: A calibrated survey meter was used for the photon measurements to obtain reliable results. A neutron detector was used as the measuring instrument for neutrons. The locations of the survey and measurements were planned carefully in order to get a proper evaluation of the facility shielding configuration. The walls, ceiling, vault entrance, and the adjacent environment were each surveyed with suitable measurement instruments. A total of 22 locations were chosen for radiation survey. Dose equivalent values were calculated for both the photon and the neutron radiation using measured data. Results: All measured dose values are presented in millisievert per year. The highest dose measured at the vault entrance is 0.34 mSv/year. A dedicated shielding door was not present at the time of the measurement. The vault entrance area is considered as a controlled area. The shielding design goals are not to exceed 5 mSv/year for the controlled area and 1 mSv/year the uncontrolled area. The total combined neutron and photon dose equivalent values were found to be compliant with the Louisiana Department of Environmental Quality radiation protection regulatory codes. Conclusion: In our efforts to evaluate the radiation levels at the Willis-Knighton Cancer Center proton treatment facility, we have found that all the measured values of the radiation shielding are below the critical radiation limits per year. Since the total dose measured at the vault entrance is below the shielding design goal, a shielding door is not required at this proton treatment vault.

  14. High potential application in bioremediation of selenate by Proteus hauseri strain QW4

    PubMed Central

    Khalilian, Mohaddeseh; Zolfaghari, Mohammad Reza; Soleimani, Mohammad

    2015-01-01

    Background and Objective: Selenium is essential for biological systems at low concentrations and toxic at higher levels. Heavy metals and metalloids such as selenium are major contaminants in 40% of hazardous waste sites. Thus, bioremediation has been considered as an effective means of cleaning up of selenium-contaminated sites. Materials and Methods: In this study, 30 strains were isolated from wastewater samples collected from selenium-contaminated sites in Qom, Iran using the enrichment culture technique. One bacterial strain designated QW4, identified as Proteus hauseri by morphological, biochemical and 16S rRNA gene sequencing was studied for its ability to tolerate different concentrations of sodium selenate (100–800 mM). Also, the disk diffusion method was performed to determine resistance to some antibiotics Results: Strain QW4 showed maximum minimum inhibitory concentration (MIC) to selenate (760 mM). The maximum selenate removal was exhibited at 35 °C, while the removal activity reduced by 30.7% and 37% at 25 °C and 40 °C, respectively. The optimum pH and shaking incubator for removal activity was shown to be 7.0 and 150 rpm, with 60.2% and 60.3%, respectively. This bacterial strain was resistant to some antibiotics. Conclusion: The concentration of toxic sodium selenate (1000 ?g/ml) in the supernatant of the bacterial culture medium decreased by 100% after 2 days and the color of the medium changed to red due to the formation of less toxic elemental selenium. Also, our results imply that heavy metal pollution may contribute to increased antibiotic resistance through indirect selection. PMID:26622970

  15. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...belonging to the genus Pseudomonas. Pseudomonas aeruginosa is a...hospital-acquired infections, and has been associated...infections, burn and wound infections, blood poisoning...brain membranes). Pseudomonas pseudomallei...

  16. Phylogenomics and systematics in Pseudomonas

    PubMed Central

    Gomila, Margarita; Peńa, Arantxa; Mulet, Magdalena; Lalucat, Jorge; García-Valdés, Elena

    2015-01-01

    The genus Pseudomonas currently contains 144 species, making it the genus of Gram-negative bacteria that contains the largest number of species. Currently, multilocus sequence analysis (MLSA) is the preferred method for establishing the phylogeny between species and genera. Four partial gene sequences of housekeeping genes (16S rRNA, gyrB, rpoB, and rpoD) were obtained from 112 complete or draft genomes of strains related to the genus Pseudomonas that were available in databases. These genes were analyzed together with the corresponding sequences of 133 Pseudomonas type strains of validly published species to assess their correct phylogenetic assignations. We confirmed that 30% of the sequenced genomes of non-type strains were not correctly assigned at the species level in the accepted taxonomy of the genus and that 20% of the strains were not identified at the species level. Most of these strains had been isolated and classified several years ago, and their taxonomic status has not been updated by modern techniques. MLSA was also compared with indices based on the analysis of whole-genome sequences that have been proposed for species delineation, such as tetranucleotide usage patterns (TETRA), average nucleotide identity (ANIm, based on MUMmer and ANIb, based on BLAST) and genome-to-genome distance (GGDC). TETRA was useful for discriminating Pseudomonas from other genera, whereas ANIb and GGDC clearly separated strains of different species. ANIb showed the strongest correlation with MLSA. The correct species classification is a prerequisite for most diversity and evolutionary studies. This work highlights the necessity for complete genomic sequences of type strains to build a phylogenomic taxonomy and that all new genome sequences submitted to databases should be correctly assigned to species to avoid taxonomic inconsistencies. PMID:26074881

  17. Characterization of a cDNA of peroxiredoxin II responding to hydrogen peroxide and phagocytosis in Amoeba proteus.

    PubMed

    Park, Miey; Shin, Hae J; Lee, Soo Y; Ahn, Tae I

    2005-01-01

    Phagocytic cells have defense systems against reactive oxygen species generated as the first non-specific defense mechanism against invading pathogens or microorganisms. We cloned a cDNA encoding a 21.69-kDa protein in Amoeba proteus homologous to 2-Cys peroxiredoxin (Prx-Ap). In the disk inhibition assay using H2O2 as an oxidizing agent, Escherichia coli overproducing Prx-Ap showed better viability than did E. coli transformed with pBluescript II SK for control. Monoclonal antibodies (mAb) produced against Prx-Ap reacted with a 22.5-kDa protein and several minor proteins. In Western blot analysis, levels of the 22.5-kDa protein in amoebae treated with 2-mM H2O2 for 1 h increased about 2-fold over those in control cells. Immunofluorescence scattered throughout the cytoplasm also increased after H2O2 treatment. In Northern blot analysis using the cDNA as a probe, the level of transcripts also changed with H2O2 treatment. When amoebae were fed with Tetrahymena, the intensity of immunofluorescence increased from 15 min and persisted until 2 h after phagocytosis. These results suggest that the 22.5-kDa protein of A. proteus is a Prx protein and that it has an antioxidant property responding to phagocytosis. PMID:15926998

  18. Reanalysis of the gas-cooled fast reactor experiments at the zero power facility proteus - Spectral indices

    SciTech Connect

    Perret, G.; Pattupara, R. M.; Girardin, G.; Chawla, R.

    2012-07-01

    The gas-cooled fast reactor (GCFR) concept was investigated experimentally in the PROTEUS zero power facility at the Paul Scherrer Inst. during the 1970's. The experimental program was aimed at neutronics studies specific to the GCFR and at the validation of nuclear data in fast spectra. A significant part of the program used thorium oxide and thorium metal fuel either distributed quasi-homogeneously in the reference PuO{sub 2}/UO{sub 2} lattice or introduced in the form of radial and axial blanket zones. Experimental results obtained at the time are still of high relevance in view of the current consideration of the Gas-cooled Fast Reactor (GFR) as a Generation-IV nuclear system, as also of the renewed interest in the thorium cycle. In this context, some of the experiments have been modeled with modern Monte Carlo codes to better account for the complex PROTEUS whole-reactor geometry and to allow validating recent continuous neutron cross-section libraries. As a first step, the MCNPX model was used to test the JEFF-3.1, JEFF-3.1.1, ENDF/B-VII.0 and JENDL-3.3 libraries against spectral indices, notably involving fission and capture of {sup 232}Th and {sup 237}Np, measured in GFR-like lattices. (authors)

  19. Characterization of hepatic growth hormone binding sites in two fish species, Gillichthys mirabilis (Teleostei) and Acipenser transmontanus (Chondrostei).

    PubMed

    Tarpey, J F; Nicoll, C S

    1985-10-01

    To obtain information on the presence of growth hormone (GH) receptors in liver of nonmammalian vertebrates the specific binding of 125I-bovine growth hormone (bGH) to liver membranes of seven species representing the major groups was studied by radioreceptor assay. A substantial degree of specific binding was detected with sturgeon (Acipenser transmontanus) liver membranes and a much lower amount was detected on hepatic membranes of Gillichthys mirabilis. No significant specific binding was detected on liver membranes of pigeon, turtle, bullfrog, tilapia, or leopard shark. Gillichthys and sturgeon liver membranes were further characterized and compared with hepatic membranes from male rabbits. The sturgeon and Gillichthys membranes showed binding that was dependent upon time, temperature, pH, and membrane concentration. Scatchard analysis of the binding of 125I-bGH to sturgeon and rabbit membranes revealed both high and low affinity binding sites. The high affinity sites had KA values of 3.1 X 10(11) and 1.0 X 10(11) M-1, and capacities of 12 and 50 fmol/mg protein, respectively. Membranes from Gillichthys liver contained only a single class of binding sites with a KA of 6.7 X 10(9) M-1 and a binding capacity of 49 fmol/mg. Hormonal specificity of the sturgeon and Gillichthys hepatic binding sites was studied using methionyl-human GH (met-hGH), ovine prolactin (oPRL), and a crude preparation of sturgeon (st)GH. The met-hGH and stGH inhibited the binding of 125I-bGH to sturgeon liver membranes while only met-hGH displaced labeled bGH from Gillichthys liver membranes. One microgram of oPRL did not significantly inhibit 125I-bGH binding in either membrane assay. Based on these studies, sturgeon hepatic GH receptors seem to be more like those of nonprimate mammals than those of teleosts. Our results, in conjunction with the data of J. N. Fryer (Gen. Comp. Endocrinol. 39, 123-130 (1979)), indicate that considerable evolutionary divergence has occurred among teleost hepatic GH receptors. Thus, vertebrate GH receptors seem to have undergone at least as much evolution as has the hormone itself. PMID:2996975

  20. The effect of leaf biopesticide Mirabilis jalapa and fungi Metarhizium anisopliae to immune response and mortality of Spodoptera exigua instar IV

    NASA Astrophysics Data System (ADS)

    Suryani, A. Irma; Anggraeni, Tjandra

    2014-03-01

    Spodoptera exigua is one of insect causing damage in agriculture sector. This insect can be controlled by a natural biopesticide by combining two agents of biological control, biopesticides Mirabilis jalapa and entomopathogenic fungi Metarhizium anisopliae, considered to be virulent toward a wide range of insects. The objective of research was to determine the effect of biopesticides M. jalapa and fungi M. anisopliae against immune system and mortality of S. exigua. This research used a complete randomized block design with five concentrations Mirabilis jalapa and optimum dose of M. anisopliae. A high dose of M. jalapa (0.8% w/v) is the most effective one to decrease total haemocytes especially granulocyt and plasmatocyt (cellular immune) and decrease the concentration of lectin (humoral immune) from S. exigua (p < 0.05). The combination of M. jalapa (0, 8% w/v) and lethal dose of M. anisopliae 2.59 × 107 spore/ml were significant to increase mortality of S. exigua within 48 hours (p < 0.05).

  1. Preparation, characterization and antibacterial activity of biodegradable films prepared from carrageenan.

    PubMed

    El-Fawal, G

    2014-09-01

    Carrageenan films have been formulated as a packaging material. Films plasticized with glycerol were loaded with citric acid (1, 0.75, 0.5, 0.25 and 0.1 %) for enhanced antimicrobial effects. Blank and citric acid loaded films were characterized by mechanical properties, scanning electron microscopy and contact angle. In addition, swelling and antibacterial studies were conducted to further characterize the films. Both blank and citric acid loaded films showed different morphology, high elasticity and acceptable tensile (mechanical) properties. These citric acid loaded films produced higher zones of inhibition against Staphylococcus aureus, Proteus mirabilis, Pseudomonas aeruginosa, Escherichia coli and Dickeya chrysanthemi strains compared to blank film. PMID:25190889

  2. FY2012 summary of tasks completed on PROTEUS-thermal work.

    SciTech Connect

    Lee, C.H.; Smith, M.A.

    2012-06-06

    PROTEUS is a suite of the neutronics codes, both old and new, that can be used within the SHARP codes being developed under the NEAMS program. Discussion here is focused on updates and verification and validation activities of the SHARP neutronics code, DeCART, for application to thermal reactor analysis. As part of the development of SHARP tools, the different versions of the DeCART code created for PWR, BWR, and VHTR analysis were integrated. Verification and validation tests for the integrated version were started, and the generation of cross section libraries based on the subgroup method was revisited for the targeted reactor types. The DeCART code has been reorganized in preparation for an efficient integration of the different versions for PWR, BWR, and VHTR analysis. In DeCART, the old-fashioned common blocks and header files have been replaced by advanced memory structures. However, the changing of variable names was minimized in order to limit problems with the code integration. Since the remaining stability problems of DeCART were mostly caused by the CMFD methodology and modules, significant work was performed to determine whether they could be replaced by more stable methods and routines. The cross section library is a key element to obtain accurate solutions. Thus, the procedure for generating cross section libraries was revisited to provide libraries tailored for the targeted reactor types. To improve accuracy in the cross section library, an attempt was made to replace the CENTRM code by the MCNP Monte Carlo code as a tool obtaining reference resonance integrals. The use of the Monte Carlo code allows us to minimize problems or approximations that CENTRM introduces since the accuracy of the subgroup data is limited by that of the reference solutions. The use of MCNP requires an additional set of libraries without resonance cross sections so that reference calculations can be performed for a unit cell in which only one isotope of interest includes resonance cross sections, among the isotopes in the composition. The OECD MHTGR-350 benchmark core was simulated using DeCART as initial focus of the verification/validation efforts. Among the benchmark problems, Exercise 1 of Phase 1 is a steady-state benchmark case for the neutronics calculation for which block-wise cross sections were provided in 26 energy groups. This type of problem was designed for a homogenized geometry solver like DIF3D rather than the high-fidelity code DeCART. Instead of the homogenized block cross sections given in the benchmark, the VHTR-specific 238-group ENDF/B-VII.0 library of DeCART was directly used for preliminary calculations. Initial results showed that the multiplication factors of a fuel pin and a fuel block with or without a control rod hole were off by 6, -362, and -183 pcm Dk from comparable MCNP solutions, respectively. The 2-D and 3-D one-third core calculations were also conducted for the all-rods-out (ARO) and all-rods-in (ARI) configurations, producing reasonable results. Figure 1 illustrates the intermediate (1.5 eV - 17 keV) and thermal (below 1.5 eV) group flux distributions. As seen from VHTR cores with annular fuels, the intermediate group fluxes are relatively high in the fuel region, but the thermal group fluxes are higher in the inner and outer graphite reflector regions than in the fuel region. To support the current project, a new three-year I-NERI collaboration involving ANL and KAERI was started in November 2011, focused on performing in-depth verification and validation of high-fidelity multi-physics simulation codes for LWR and VHTR. The work scope includes generating improved cross section libraries for the targeted reactor types, developing benchmark models for verification and validation of the neutronics code with or without thermo-fluid feedback, and performing detailed comparisons of predicted reactor parameters against both Monte Carlo solutions and experimental measurements. The following list summarizes the work conducted so far for PROTEUS-Thermal Tasks: Unification of different versions of DeC

  3. Genomics Of Pseudomonas Fluorescens Pf-5

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three complete genomic sequences for five species of Pseudomonas and three strains of Pseudomonas fluorescens are now available. Comparisons of the genomic sequences highlight the diversity of the genus, as well as among strains of P. fluorescens. This chapter focuses on Pf-5, a rhizosphere bacter...

  4. Pseudomonas--an opportunistic foe.

    PubMed

    Baillie, Jonathan

    2014-01-01

    An honest account of some of the lessons learned in how to protect patients, staff, and visitors, against waterborne Pseudomonas aeruginosa by effectively monitoring a large healthcare facility's water supply, identifying potential 'trigger points', harnessing the expertise of a multidisciplinary team, encouraging all staff to 'go the extra mile' preventatively, and above all, 'going beyond compliance', was provided by George McCracken, head of Estates Risk and Environment at the Belfast Health and Social Care Trust--in whose Royal Jubilee Maternity Hospital three young babies died after an outbreak of the bacteraemia in early 2012--at a recent Water Management Society conference. HEJ editor, Jonathan Baillie, reports. PMID:24516937

  5. Ferrofluid effect on Pseudomonas pyoverdine

    NASA Astrophysics Data System (ADS)

    Poiata, Antoniea; Vlahovici, Al.; Creanga, Dorina-Emilia

    2005-03-01

    The magnetic fluid effect on some pigmented pathogen germs has been investigated. The fluorescence of the pyoverdine pigment obtained from Pseudomonas aeruginosa strain, cultivated in the presence of different magnetic fluid concentrations, was enhanced by magnetic fluid concentrations of 0.0015-1 ml/l. The antimicrobial activity of pyoverdine, when tested by means of agar diffusimetric method against Sarcina lutea, was found increased for relatively high concentrations of magnetic fluid; in the case of Staphylococcus aureus the pyoverdine antimicrobial activity was not dependent on the magnetic fluid concentration.

  6. In the First International Conference on Web Services (ICWS), Las Vegas, Nevada, June 23-26, 2003. Proteus: A System for Dynamically Composing and Intelligently Executing Web

    E-print Network

    Papadopoulos, Christos

    In the First International Conference on Web Services (ICWS), Las Vegas, Nevada, June 23-26, 2003. 1 Proteus: A System for Dynamically Composing and Intelligently Executing Web Services1 Shahram at will and predict the future. Abstract Many organizations envision web services as an enabling component of Internet

  7. Antimicrobial activity of two essential oils.

    PubMed

    Mickien?, R?ta; Bakutis, Bronius; Baliukonien?, Violeta

    2011-06-01

    The aim of the study was to evaluate the antimicrobial activity of essential oils in vitro for possible application to reduce the content of microorganisms in the air of animal houses. The essential oils of Cymbopogon citrarus L. and Malaleuca alternifolia L. were screened against bacteria Staphylococcus aureus, Enterococcus faecium, Pseudomonas aeruginosa, Escherichia coli, Proteus mirabilis and yeast Candida albicans. The minimal inhibitory concentration of the active essential oils was tested using broth dilution assay. The essential oils concentrations ranged from 0.1-50.0%. The combined effects of essential oils were tested for Malaleuca alternifolia L. and Cymbopogon citrarus L. concentrations ranged from 0.005-50.0%. The oils showed a wide spectrum of antibacterial activity. Concentrations of 0.1-0.5% of Cymbopogon citrarus L. and Malaleuca alternifolia L. reduced total microorganisms count of Proteus mirabilis and Candida albicans. High antibacterial activity was also revealed for Cymbopogon citrarus L. with bactericidal concentrations of 0.8% for Escherichia coli, 5.0% for Enterococcus faecium, 5.0% for Pseudomonas aeruginosa and 8.0% for Staphylococcus aureus. Bactericidal concentrations of Malaleuca alternifolia L. were 5.0% for Pseudomonas aeruginosa and Enterococcus faecium, and 8.0% for Staphylococcus aureus. The essential oils of Cymbopogon citrarus and Malaleuca alternifolia may be a promising alternative of air disinfection in animal houses. PMID:21739934

  8. Welwitschia mirabilis

    E-print Network

    Leon Pratorius

    2011-08-10

    at Beaumont, Eagle Lake, Ganado and in commercial rice fields this year. Experiments are being conducted on stem borers, rice stink bug, chinch bug, aphids and rice water weevil. We are evaluating new insecticidal seed treatments to replace Icon. Some... to stem borers. We are developing an easier method for sampling rice stink bug and evaluating rice stink bug residual control using tank mixes of various surfactants/oils and methyl parathion. We are determining the effects of bird cherry oat aphid...

  9. HTR-Proteus Pebble Bed Experimental Program Cores 5,6,7,&8: Columnar Hexagonal Point-on-Point Packing with a 1:2 Moderator-to-Fuel Pebble Ratio

    SciTech Connect

    Bess, John D.; Sterbentz, James W.; Snoj, Luka; Lengar, Igor; Koberl, Oliver

    2015-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  10. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORES 5, 6, 7, & 8: COLUMNAR HEXAGONAL POINT-ON-POINT PACKING WITH A 1:2 MODERATOR-TO-FUEL PEBBLE RATIO

    SciTech Connect

    John D. Bess

    2013-03-01

    PROTEUS is a zero-power research reactor based on a cylindrical graphite annulus with a central cylindrical cavity. The graphite annulus remains basically the same for all experimental programs, but the contents of the central cavity are changed according to the type of reactor being investigated. Through most of its service history, PROTEUS has represented light-water reactors, but from 1992 to 1996 PROTEUS was configured as a pebble-bed reactor (PBR) critical facility and designated as HTR-PROTEUS. The nomenclature was used to indicate that this series consisted of High Temperature Reactor experiments performed in the PROTEUS assembly. During this period, seventeen critical configurations were assembled and various reactor physics experiments were conducted. These experiments included measurements of criticality, differential and integral control rod and safety rod worths, kinetics, reaction rates, water ingress effects, and small sample reactivity effects (Ref. 3). HTR-PROTEUS was constructed, and the experimental program was conducted, for the purpose of providing experimental benchmark data for assessment of reactor physics computer codes. Considerable effort was devoted to benchmark calculations as a part of the HTR-PROTEUS program. References 1 and 2 provide detailed data for use in constructing models for codes to be assessed. Reference 3 is a comprehensive summary of the HTR-PROTEUS experiments and the associated benchmark program. This document draws freely from these references. Only Cores 9 and 10 are evaluated in this benchmark report due to similarities in their construction. The other core configurations of the HTR-PROTEUS program are evaluated in their respective reports as outlined in Section 1.0. Cores 9 and 10 were evaluated and determined to be acceptable benchmark experiments.

  11. A comparative study of nemertean complete mitochondrial genomes, including two new ones for Nectonemertes cf. mirabilis and Zygeupolia rubens, may elucidate the fundamental pattern for the phylum Nemertea

    PubMed Central

    2012-01-01

    Background The mitochondrial genome is important for studying genome evolution as well as reconstructing the phylogeny of organisms. Complete mitochondrial genome sequences have been reported for more than 2200 metazoans, mainly vertebrates and arthropods. To date, from a total of about 1275 described nemertean species, only three complete and two partial mitochondrial DNA sequences from nemerteans have been published. Here, we report the entire mitochondrial genomes for two more nemertean species: Nectonemertes cf. mirabilis and Zygeupolia rubens. Results The sizes of the entire mitochondrial genomes are 15365 bp for N. cf. mirabilis and 15513 bp for Z. rubens. Each circular genome contains 37 genes and an AT-rich non-coding region, and overall nucleotide composition is AT-rich. In both species, there is significant strand asymmetry in the distribution of nucleotides, with the coding strand being richer in T than A and in G than C. The AT-rich non-coding regions of the two genomes have some repeat sequences and stem-loop structures, both of which may be associated with the initiation of replication or transcription. The 22 tRNAs show variable substitution patterns in nemerteans, with higher sequence conservation in genes located on the H strand. Gene arrangement of N. cf. mirabilis is identical to that of Paranemertes cf. peregrina, both of which are Hoplonemertea, while that of Z. rubens is the same as in Lineus viridis, both of which are Heteronemertea. Comparison of the gene arrangements and phylogenomic analysis based on concatenated nucleotide sequences of the 12 mitochondrial protein-coding genes revealed that species with closer relationships share more identical gene blocks. Conclusion The two new mitochondrial genomes share many features, including gene contents, with other known nemertean mitochondrial genomes. The tRNA families display a composite substitution pathway. Gene order comparison to the proposed ground pattern of Bilateria and some lophotrochozoans suggests that the nemertean ancestral mitochondrial gene order most closely resembles the heteronemertean type. Phylogenetic analysis proposes a sister-group relationship between Hetero- and Hoplonemertea, which supports one of two recent alternative hypotheses of nemertean phylogeny. PMID:22507536

  12. Within-Pin Reaction Rate Distributions: CASMO-4 and HELIOS Compared Against Tomographic Measurements at the PROTEUS Reactor

    SciTech Connect

    Fauchere, C. Pralong; Murphy, M.; Jatuff, F.; Chawla, R.

    2005-05-15

    In the framework of the LWR-PROTEUS project - an extended validation program for advanced light water reactor core analysis tools conducted at the Paul Scherrer Institute - the radial, internal variations of the total fission rate (F{sub tot}) and the capture rate in {sup 238}U (C{sub 8}) have been calculated for zero-burnup pins of a Westinghouse SVEA-96+ boiling water reactor fuel assembly using two codes, namely, CASMO-4 and HELIOS. While F{sub tot} distributions predicted by CASMO-4 and HELIOS are in good agreement, C{sub 8} distributions show significant inconsistencies (20 to 30%). The calculations are compared with experimental results obtained using single photon emission computerized tomography for several SVEA-96+ pins irradiated in the zero-power reactor PROTEUS. The comparisons confirm the predicted shape of the F{sub tot} distributions within UO{sub 2} pins and clearly indicate that HELIOS within-pin predictions for C{sub 8} are more reliable than CASMO-4 results. This is important for the derivation of gamma-ray self-absorption corrections when pin-integrated reaction rates are to be determined using the gamma-scanning technique. Thus, the use of CASMO-4-type within-pin distributions would lead to 3 to 4% discrepancies in the absolute, self-absorption-corrected pin-integrated values deduced for C{sub 8} and hence for C{sub 8}/F{sub tot}. For relative C{sub 8} distributions, the discrepancy would be much smaller, namely, up to {approx}1% if pins containing a burnable absorber are involved.

  13. Design of a proteus lattice representative of a burnt and fresh fuel interface at power conditions in light water reactors

    SciTech Connect

    Hursin, M.; Perret, G.

    2012-07-01

    The research program LIFE (Large-scale Irradiated Fuel Experiment) between PSI and Swissnuclear has been started in 2006 to study the interaction between large sets of burnt and fresh fuel pins in conditions representative of power light water reactors. Reactor physics parameters such as flux ratios and reaction rate distributions ({sup 235}U and {sup 238}U fissions and {sup 238}U capture) are calculated to estimate an appropriate arrangement of burnt and fresh fuel pins within the central element of the test zone of the zero-power research reactor PROTEUS. The arrangement should minimize the number of burnt fuel pins to ease fuel handling and reduce costs, whilst guaranteeing that the neutron spectrum in both burnt and fresh fuel regions and at their interface is representative of a large uniform array of burnt and fresh pins in the same moderation conditions. First results are encouraging, showing that the burnt/fresh fuel interface is well represented with a 6 x 6 bundle of burnt pins. The second part of the project involves the use of TSUNAMI, CASMO-4E and DAKOTA to perform parametric and optimization studies on the PROTEUS lattice by varying its pitch (P) and fraction of D{sub 2}O in moderator (F{sub D2O}) to be as representative as possible of a power light water reactor core at hot full power conditions at beginning of cycle (BOC). The parameters P and F{sub D2O} that best represent a PWR at BOC are 1.36 cm and 5% respectively. (authors)

  14. Allison Lab Protocol, Pseudomonas Culturing, 4/2008, Steve Allison Reagents and Procedures for Pseudomonas Culturing

    E-print Network

    German, Donovan P.

    . fluorescens mutant J-1, add 100 µg/ml rifampin and 50 µg/ml kanamycin #12;Allison Lab Protocol, PseudomonasAllison Lab Protocol, Pseudomonas Culturing, 4/2008, Steve Allison Reagents and Procedures for Pseudomonas Culturing 1 M MgSO4 Dissolve 24.6 g MgSO4·7H2O in 100 ml DI water. Autoclave for 20 min. 1 M Tris

  15. 21 CFR 520.88b - Amoxicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary tract (cystitis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract (bacterial gastroenteritis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis caused by S....

  16. 21 CFR 520.88b - Amoxicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary tract (cystitis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract (bacterial gastroenteritis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis caused by S....

  17. 21 CFR 520.88b - Amoxicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary tract (cystitis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract (bacterial gastroenteritis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis caused by S....

  18. 21 CFR 520.88b - Amoxicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary tract (cystitis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract (bacterial gastroenteritis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis caused by S....

  19. 21 CFR 520.88b - Amoxicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Streptococcus spp., Escherichia coli, and Proteus mirabilis; genitourinary tract (cystitis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract (bacterial gastroenteritis) caused by S. aureus, Streptococcus spp., E. coli, and P. mirabilis; bacterial dermatitis caused by S....

  20. Antibiotic Conditioned Growth Medium of Pseudomonas Aeruginosa

    ERIC Educational Resources Information Center

    Benathen, Isaiah A.; Cazeau, Barbara; Joseph, Njeri

    2004-01-01

    A simple method to study the consequences of bacterial antibiosis after interspecific competition between microorganisms is presented. Common microorganisms are used as the test organisms and Pseudomonas aeruginosa are used as the source of the inhibitor agents.

  1. Hot Tub Rash (Pseudomonas Dermatitis/Folliculitis)

    MedlinePLUS

    ... How Do I Protect Myself and My Family? "Hot Tub Rash" ( Pseudomonas Dermatitis / Folliculitis) Below are answers ... hot tub rash and healthy swimming. What is Hot Tub Rash? Hot tub rash, or dermatitis, is ...

  2. Strains of Pseudomonas putrefaciens from clinical material.

    PubMed Central

    Holmes, B; Lapage, S P; Malnick, H

    1975-01-01

    Eight strains of Pseudomonas putrefaciens have been received from among 466 strains of Pseudomonas submitted to the Computer Trials Laboratory for identification over the last eight years. Two of the strains of P. putrefaciens from patients with otitis media and otitis externa respectively appear to have played a pathogenic role. The biochemical characteristics of these eight strains were compared with those of seven culture collection strains. PMID:1127110

  3. OXIDATION OF POLYCHLORINATED BIPHENYLS BY PSEUDOMONAS SP. STRAIN LB400 AND PSEUDOMONAS PSEUDOALCALIGENES KF707

    EPA Science Inventory

    Biphenyl-grown cells and cell extracts prepared from biphenyl-grown cells of Pseudomonas sp. strain LB400 oxidize a much wider range of chlorinated biphenyls than do analogous preparations from Pseudomonas pseudoalcaligenes KF707. These results are attributed to differences in th...

  4. Results of the Simulation of the HTR-Proteus Core 4.2 Using PEBBED-COMBINE: FY10 Report

    SciTech Connect

    Hans Gougar

    2010-07-01

    ABSTRACT The Idaho National Laboratory’s deterministic neutronics analysis codes and methods were applied to the computation of the core multiplication factor of the HTR-Proteus pebble bed reactor critical facility. This report is a follow-on to INL/EXT-09-16620 in which the same calculation was performed but using earlier versions of the codes and less developed methods. In that report, results indicated that the cross sections generated using COMBINE-7.0 did not yield satisfactory estimates of keff. It was concluded in the report that the modeling of control rods was not satisfactory. In the past year, improvements to the homogenization capability in COMBINE have enabled the explicit modeling of TRIS particles, pebbles, and heterogeneous core zones including control rod regions using a new multi-scale version of COMBINE in which the 1-dimensional discrete ordinate transport code ANISN has been integrated. The new COMBINE is shown to yield benchmark quality results for pebble unit cell models, the first step in preparing few-group diffusion parameters for core simulations. In this report, the full critical core is modeled once again but with cross sections generated using the capabilities and physics of the improved COMBINE code. The new PEBBED-COMBINE model enables the exact modeling of the pebbles and control rod region along with better approximation to structures in the reflector. Initial results for the core multiplication factor indicate significant improvement in the INL’s tools for modeling the neutronic properties of a pebble bed reactor. Errors on the order of 1.6-2.5% in keff are obtained; a significant improvement over the 5-6% error observed in the earlier This is acceptable for a code system and model in the early stages of development but still too high for a production code. Analysis of a simpler core model indicates an over-prediction of the flux in the low end of the thermal spectrum. Causes of this discrepancy are under investigation. New homogenization techniques and assumptions were used in this analysis and as such, they require further confirmation and validation. Further refinement and review of the complex Proteus core model are likely to reduce the errors even further.

  5. Social Dynamics in Natural Populations of Pseudomonas aeruginosa

    E-print Network

    West, Stuart

    Social Dynamics in Natural Populations of Pseudomonas aeruginosa Melanie Ghoul Merton. Loss of Social Behaviours in Populations of Pseudomonas aeruginosa Infecting Lungs the bacterial genomes and annotated them with all ribosomal and bacterial multilocus sequence typing DNA

  6. Combining Genomic Tools to Dissect Multifactorial Virulence in Pseudomonas

    E-print Network

    Ausubel, Frederick M.

    Combining Genomic Tools to Dissect Multifactorial Virulence in Pseudomonas aeruginosa Daniel G. Lee- ronmental bacterium and important opportunistic human pathogen, Pseudomonas aeruginosa. By comparing PA14 Introduction The growing number of sequenced bacterial genomes, including those of important pathogenic

  7. Bacterial Uropathogens Isolates and Antibiograms in Children Under 5 Years of Age

    PubMed Central

    Alsammani, Mohamed Alkhatim; Ahmed, Mohamed Issa; Abdelatif, Nahla Farouk

    2014-01-01

    Background: Childhood urinary infections are among the most common febrile illnesses occurring during this period with varying susceptibility to antibiotic. Aim: The aim of this study was to identify uropathogens responsible to for urinarytract infection (UTIs) in children less than 5 years of age, and determine the antibiograms of the isolates to commonly used antibiotics. Patients and methods: Hundred and four children (2 months - 5 years old) seen at the Gadarif Teaching Hospital from January 2012 and December 2013 were evaluated. A urine specimen was obtained by a plastic bag with an adhesive backing around an opening or by direct voiding into sterile container. Urine was examined microscopically and those with significant pyuria and bacteruria were further cultured and microorganisms were identified and tested for antimicrobial susceptibility. Results: Out of 304 children suffering from UTIs; 145(47.7%) had significant pyuria of them; 54(17.8 %) had positive bacterial growth. The frequency of sex and residency were almost the same. E. coli (42.6%) was the most common uropathogen, sensitive to ciprofloxacin (91.3%), followed by Pseudomonas aeruginosa (29.6%) sensitive to Ciprofloxacin (75%)and Norofloxacin (68.8%), Klebsiellapneumoniae (18.5%) sensitive to Ciprofloxacin and Norofloxacin and Nalidixic acid (90%) and Proteus mirabilis sensitive to Ciprofloxacin and Norofloxacin (90%), Amoxicillin / clavulanic acid (Augmentin(80%). Conclusion: The most common uropathogens were E. coli, Pseudomonas aeruginosa,Klebsiellapneumoniae, and Proteus mirabilis. Ciprofloxacin is the recommended initial empirical therapy while awaiting the culture and sensitivity results. PMID:25568544

  8. Capsule production by Pseudomonas aeruginosa

    SciTech Connect

    Lynn, A.R.

    1984-01-01

    Mucoid strains of Pseudomonas aeruginosa, associated almost exclusively with chronic respiratory infections in patients with cystic fibrosis, possess a capsule composed of alginic acid similar to one produced by Azotobacter vinelandii. Recent reports have provided evidence that the biosynthetic pathway for alginate in P. aeruginosa may differ from the pathway proposed for A. vinelandii in that synthesis in P. aeruginosa may occur by way of the Entner-Doudoroff pathway. Incorporation of isotope from (6-/sup 14/C)glucose into alginate by both P. aueroginosa and A. vinelandii was 10-fold greater than that from either (1-/sup 14/C)/sup -/ or (2-/sup 14/C)glucose, indicating preferential utilization of the bottom half of the glucose molecule for alginate biosynthesis. These data strongly suggest that the Entner-Doudoroff pathway plays a major role in alginate synthesis in both P. aeruginosa and A. vinelandii. The enzymes of carbohydrate metabolism in mucoid strains of P. aeruginosa appear to be unchanged whether alignate is actively produced or not and activities do not differ significantly from nonmucoid strain PAO.

  9. Glycerophospholipid synthesis and functions in Pseudomonas.

    PubMed

    Kondakova, Tatiana; D'Heygčre, François; Feuilloley, Marc J; Orange, Nicole; Heipieper, Hermann J; Duclairoir Poc, Cécile

    2015-09-01

    The genus Pseudomonas is one of the most heterogeneous groups of eubacteria, presents in all major natural environments and in wide range of associations with plants and animals. The wide distribution of these bacteria is due to the use of specific mechanisms to adapt to environmental modifications. Generally, bacterial adaptation is only considered under the aspect of genes and protein expression, but lipids also play a pivotal role in bacterial functioning and homeostasis. This review resumes the mechanisms and regulations of pseudomonal glycerophospholipid synthesis, and the roles of glycerophospholipids in bacterial metabolism and homeostasis. Recently discovered specific pathways of P. aeruginosa lipid synthesis indicate the lineage dependent mechanisms of fatty acids homeostasis. Pseudomonas glycerophospholipids ensure structure functions and play important roles in bacterial adaptation to environmental modifications. The lipidome of Pseudomonas contains a typical eukaryotic glycerophospholipid--phosphatidylcholine -, which is involved in bacteria-host interactions. The ability of Pseudomonas to exploit eukaryotic lipids shows specific and original strategies developed by these microorganisms to succeed in their infectious process. All compiled data provide the demonstration of the importance of studying the Pseudomonas lipidome to inhibit the infectious potential of these highly versatile germs. PMID:26148574

  10. Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous

    E-print Network

    Tawfik, Dan S.

    Directed evolution of phosphotriesterase from Pseudomonas diminuta for heterologous expression should be addressed. E-mail: tawfik@weizmann.ac.il Phosphotriesterase from Pseudomonas diminuta (PTE isolated from the soil bac- terium Pseudomonas diminuta, catalyses the detoxification of a wide variety

  11. ORIGINAL RESEARCH Expression of Fap amyloids in Pseudomonas aeruginosa,

    E-print Network

    Schierup, Mikkel Heide

    ORIGINAL RESEARCH Expression of Fap amyloids in Pseudomonas aeruginosa, P. fluorescens, and P, Aarhus C, Denmark Keywords Amyloids, biofilm, fap, FuBA, Pseudomonas.. Correspondence Per H. Nielsen­382 doi: 10.1002/mbo3.81 Abstract The fap operon, encoding functional amyloids in Pseudomonas (Fap

  12. Pseudomonas syringae Hrp type III secretion system and effector proteins

    E-print Network

    and Pseudomonas fluorescens to inject HopPsyA into tobacco cells, thereby eliciting a hypersensitive responseColloquium Pseudomonas syringae Hrp type III secretion system and effector proteins Alan Collmer; and ¶Department of Biological Sciences, University of Nevada, Las Vegas, NV 89154-4004 Pseudomonas syringae

  13. Proteus two-dimensional Navier-Stokes computer code, version 2.0. Volume 2: User's guide

    NASA Technical Reports Server (NTRS)

    Towne, Charles E.; Schwab, John R.; Bui, Trong T.

    1993-01-01

    A computer code called Proteus 2D was developed to solve the two-dimensional planar or axisymmetric, Reynolds-averaged, unsteady compressible Navier-Stokes equations in strong conservation law form. The objective in this effort was to develop a code for aerospace propulsion applications that is easy to use and easy to modify. Code readability, modularity, and documentation were emphasized. The governing equations are solved in generalized nonorthogonal body-fitted coordinates, by marching in time using a fully-coupled ADI solution procedure. The boundary conditions are treated implicitly. All terms, including the diffusion terms, are linearized using second-order Taylor series expansions. Turbulence is modeled using either an algebraic or two-equation eddy viscosity model. The thin-layer or Euler equations may also be solved. The energy equation may be eliminated by the assumption of constant total enthalpy. Explicit and implicit artificial viscosity may be used. Several time step options are available for convergence acceleration. The documentation is divided into three volumes. This is the User's Guide, and describes the program's features, the input and output, the procedure for setting up initial conditions, the computer resource requirements, the diagnostic messages that may be generated, the job control language used to run the program, and several test cases.

  14. Proteus two-dimensional Navier-Stokes computer code, version 2.0. Volume 3: Programmer's reference

    NASA Technical Reports Server (NTRS)

    Towne, Charles E.; Schwab, John R.; Bui, Trong T.

    1993-01-01

    A computer code called Proteus 2D was developed to solve the two-dimensional planar or axisymmetric, Reynolds-averaged, unsteady compressible Navier-Stokes equations in strong conservation law form. The objective in this effort was to develop a code for aerospace propulsion applications that is easy to use and easy to modify. Code readability, modularity, and documentation were emphasized. The governing equations are solved in generalized nonorthogonal body-fitted coordinates, by marching in time using a fully-coupled ADI solution procedure. The boundary conditions are treated implicitly. All terms, including the diffusion terms, are linearized using second-order Taylor series expansions. Turbulence is modeled using either an algebraic or two-equation eddy viscosity model. The thin-layer or Euler equations may also be solved. The energy equation may be eliminated by the assumption of constant total enthalpy. Explicit and implicit artificial viscosity may be used. Several time step options are available for convergence acceleration. The documentation is divided into three volumes. The Programmer's Reference contains detailed information useful when modifying the program. The program structure, the Fortran variables stored in common blocks, and the details of each subprogram are described.

  15. Proteus three-dimensional Navier-Stokes computer code, version 1.0. Volume 1: Analysis description

    NASA Technical Reports Server (NTRS)

    Towne, Charles E.; Schwab, John R.; Bui, Trong T.

    1993-01-01

    A computer code called Proteus 3D has been developed to solve the three dimensional, Reynolds averaged, unsteady compressible Navier-Stokes equations in strong conservation law form. The objective in this effort has been to develop a code for aerospace propulsion applications that is easy to use and easy to modify. Code readability, modularity, and documentation have been emphasized. The governing equations are solved in generalized non-orthogonal body-fitted coordinates by marching in time using a fully-coupled ADI solution procedure. The boundary conditions are treated implicitly. All terms, including the diffusion terms, are linearized using second-order Taylor series expansions. Turbulence is modeled using either an algebraic or two-equation eddy viscosity model. The thin-layer or Euler equations may also be solved. The energy equation may be eliminated by the assumption of constant total enthalpy. Explicit and implicit artificial viscosity may be used. Several time step options are available for convergence acceleration. The documentation is divided into three volumes. This is the Analysis Description, and presents the equations and solution procedure. It describes in detail the governing equations, the turbulence model, the linearization of the equations and boundary conditions, the time and space differencing formulas, the ADI solution procedure, and the artificial viscosity models.

  16. Investigation of the void coefficient and other integral parameters in the PROTEUS-LWHCR phase II program

    SciTech Connect

    Seiler, R.; Chawla, R.; Gmur, K.; Hager, H.; Berger, H.D.; Bohme, R.

    1988-02-01

    Comparisons of calculated and measured neutron balance components are reported for the 7.5% fissile plutonium reference test lattice of the PROTEUS-light water high conversion reactor (LWHCR) phase II program, both wet (with H/sub 2/O) and dry (100% void). Special experimental techniques have been developed and applied, particularly for kappa/sub infinity/, and the range of directly measured reaction rate ratios has been extended. For the two cell codes tested, WIMS-D/1981 library and KARBUS/KEDAK-4, specific shortcomings have been identified; the new measurements have been found to be significantly more representative and accurate than the earlier phase I experiments. The kappa/sub infinity/ void coefficient for the phase II reference lattice between 0 and 100% void has been found to be qualitatively different from those assessed for the earlier phase I test lattices. Consideration of the individual void coefficient components show this to be largely a consequence of the more LWHCR-representative fuel rod diameter and plutonium isotopic composition of the fuel currently being used. Results of control rod studies conducted for the phase II reference lattice - both wet and dry - serve to illustrate the efforts being made toward investigations of special power reactor features.

  17. Proteus two-dimensional Navier-Stokes computer code, version 2.0. Volume 1: Analysis description

    NASA Technical Reports Server (NTRS)

    Towne, Charles E.; Schwab, John R.; Bui, Trong T.

    1993-01-01

    A computer code called Proteus 2D was developed to solve the two-dimensional planar or axisymmetric, Reynolds-averaged, unsteady compressible Navier-Stokes equations in strong conservation law form. The objective in this effort was to develop a code for aerospace propulsion applications that is easy to use and easy to modify. Code readability, modularity, and documentation were emphasized. The governing equations are solved in generalized nonorthogonal body-fitted coordinates, by marching in time using a fully-coupled ADI solution procedure. The boundary conditions are treated implicitly. All terms, including the diffusion terms, are linearized using second-order Taylor series expansions. Turbulence is modeled using either an algebraic or two-equation eddy viscosity model. The thin-layer or Euler equations may also be solved. The energy equation may be eliminated by the assumption of constant total enthalpy. Explicit and implicit artificial viscosity may be used. Several time step options are available for convergence acceleration. The documentation is divided into three volumes. This is the Analysis Description, and presents the equations and solution procedure. The governing equations, the turbulence model, the linearization of the equations and boundary conditions, the time and space differencing formulas, the ADI solution procedure, and the artificial viscosity models are described in detail.

  18. Proteus three-dimensional Navier-Stokes computer code, version 1.0. Volume 3: Programmer's reference

    NASA Technical Reports Server (NTRS)

    Towne, Charles E.; Schwab, John R.; Bui, Trong T.

    1993-01-01

    A computer code called Proteus 3D was developed to solve the three-dimensional, Reynolds-averaged, unsteady compressible Navier-Stokes equations in strong conservation law form. The objective in this effort was to develop a code for aerospace propulsion applications that is easy to use and easy to modify. Code readability, modularity, and documentation were emphasized. The governing equations are solved in generalized nonorthogonal body fitted coordinates, by marching in time using a fully-coupled ADI solution procedure. The boundary conditions are treated implicitly. All terms, including the diffusion terms, are linearized using second-order Taylor series expansions. Turbulence is modeled using either an algebraic or two-equation eddy viscosity model. The thin-layer or Euler equations may also be solved. The energy equation may be eliminated by the assumption of constant total enthalpy. Explicit and implicit artificial viscosity may be used. Several time step options are available for convergence acceleration. The documentation is divided into three volumes. The Programmer's Reference contains detailed information useful when modifying the program. The program structure, the Fortran variables stored in common blocks, and the details of each subprogram are described.

  19. Proteus three-dimensional Navier-Stokes computer code, version 1.0. Volume 2: User's guide

    NASA Technical Reports Server (NTRS)

    Towne, Charles E.; Schwab, John R.; Bui, Trong T.

    1993-01-01

    A computer code called Proteus 3D was developed to solve the three-dimensional, Reynolds-averaged, unsteady compressible Navier-Stokes equations in strong conservation law form. The objective in this effort was to develop a code for aerospace propulsion applications that is easy to use and easy to modify. Code readability, modularity, and documentation were emphasized. The governing equations are solved in generalized nonorthogonal body-fitted coordinates, by marching in time using a fully-coupled ADI solution procedure. The boundary conditions are treated implicitly. All terms, including the diffusion terms, are linearized using second-order Taylor series expansions. Turbulence is modeled using either an algebraic or two-equation eddy viscosity model. The thin-layer or Euler equations may also be solved. The energy equation may be eliminated by the assumption of constant total enthalpy. Explicit and implicit artificial viscosity may be used. Several time step options are available for convergence acceleration. The documentation is divided into three volumes. This User's Guide describes the program's features, the input and output, the procedure for setting up initial conditions, the computer resource requirements, the diagnostic messages that may be generated, the job control language used to run the program, and several test cases.

  20. New naphthalene-degrading marine Pseudomonas strains

    SciTech Connect

    Garcia-Valdes, E.; Cozar, E.; Rotger, R. Lalucat, J. ); Ursing, J. )

    1988-10-01

    Over 100 strains that utilized naphthalene as the only carbon and energy source were isolated from samples of marine sediments taken from a heavily polluted area. The isolates were characterized taxonomically and physiologically. Most of these strains belonged to the genus Pseudomonas, and seven of them did not fit any previous taxonomic description. They differed from type strains in a few biochemical characteristics and in the utilization of aromatic compounds. None had catechol 1,2-dioxygenase activity, and catechol 2,3-dioxygenase was responsible for the aromatic ring cleavage. DNA hybridizations demonstrated a close relationship between two isolates and the Pseudomonas stutzeri type strain, and between five isolates and the Pseudomonas testosteroni type strain. On the basis of nutritional and enzymatic characteristics, it was assumed that the seven isolates represent new biovars belonging to the species P. testosteroni and P. stutzeri that are able to degrade aromatic hydrocarbons.

  1. Population structure of Pseudomonas aeruginosa

    PubMed Central

    Wiehlmann, Lutz; Wagner, Gerd; Cramer, Nina; Siebert, Benny; Gudowius, Peter; Morales, Gracia; Köhler, Thilo; van Delden, Christian; Weinel, Christian; Slickers, Peter; Tümmler, Burkhard

    2007-01-01

    The metabolically versatile Gram-negative bacterium Pseudomonas aeruginosa inhabits terrestrial, aquatic, animal-, human-, and plant-host-associated environments and is an important causative agent of nosocomial infections, particularly in intensive-care units. The population genetics of P. aeruginosa was investigated by an approach that is generally applicable to the rapid, robust, and informative genotyping of bacteria. DNA, amplified from the bacterial colony by circles of multiplex primer extension, is hybridized onto a microarray to yield an electronically portable binary multimarker genotype that represents the core genome by single nucleotide polymorphisms and the accessory genome by markers of genomic islets and islands. The 240 typed P. aeruginosa strains of diverse habitats and geographic origin segregated into two large nonoverlapping clusters and 45 isolated clonal complexes with few or no partners. The majority of strains belonged to few dominant clones widespread in disease and environmental habitats. The most frequent genotype was represented by the sequenced strain PA14. Core and accessory genome were found to be nonrandomly assembled in P. aeruginosa. Individual clones preferred a specific repertoire of accessory segments. Even the most promiscuous genomic island, pKLC102, had integrated preferentially into a subset of clones. Moreover, some physically distant loci of the core genome, including oriC, showed nonrandom associations of genotypes, whereas other segments in between were freely recombining. Thus, the P. aeruginosa genome is made up of clone-typical segments in core and accessory genome and of blocks in the core with unrestricted gene flow in the population. PMID:17468398

  2. The effect of leaf biopesticide (Mirabilis jalapa) and entomopathogenic fungi (Beauveria bassiana) combinations to some physiological characters and histology of Crocidolomia pavonana (Lepidoptera: Pyralidae) larvae

    SciTech Connect

    Sirajuddin, Nur Tasmiah Anggraeni, Tjandra

    2014-03-24

    Crocidolomia pavonana is one of the most prominent pest that cause damage to vegetables especially Brassicaceae such us cabbage, broccoli, mustard greens and turnips, these vegetable have been widely consumed and cultivated in Indonesia. The invation of this pest might created high risk of cultivated failure. Enviromentally pest control efforts by utilizing biological control agents such us biopesticides of plants and entomopathogenic fungi have been carried out, but the work was relatively long and strongly influenced by environmental factors. The purpose of this study was to combine biopesticide of Mirabilis jalapa and entomopathogenic fungi Beauveria bassiana to look at mortality of C. pavonana larvae observing by histological incision and scanning electron microscope. Concentration treatments of extracts M. jalapa was (control; 0,1; 0,2; 0,4 and 0,8 gr/ml) and the result showed that the effective concentration was 0,8 g/ml which affect significantly (P<0,05) in reduce pupa weight, improve pupasi time, lowering percentage of emergence imago and improve the long phase of pupa which differ significantly with control. The combination of biopesticides proved to accelerate the mortality of larvae. Histological incision observed at hour 24, 48, 72 and 96, where the biggest damage occurred at hour 96. Observation by scanning electron microscope showed fungus spores that attach to the body surface of larvae subsequently penetrate into the body. Thus the combination use of biopesticides M. jalapa and fungi B. bassiana, can be used as an alternative pest control C. pavonana.

  3. The effect of leaf biopesticide (Mirabilis jalapa) and entomopathogenic fungi (Beauveria bassiana) combinations to some physiological characters and histology of Crocidolomia pavonana (Lepidoptera: Pyralidae) larvae

    NASA Astrophysics Data System (ADS)

    Sirajuddin, Nur Tasmiah; Anggraeni, Tjandra

    2014-03-01

    Crocidolomia pavonana is one of the most prominent pest that cause damage to vegetables especially Brassicaceae such us cabbage, broccoli, mustard greens and turnips, these vegetable have been widely consumed and cultivated in Indonesia. The invation of this pest might created high risk of cultivated failure. Enviromentally pest control efforts by utilizing biological control agents such us biopesticides of plants and entomopathogenic fungi have been carried out, but the work was relatively long and strongly influenced by environmental factors. The purpose of this study was to combine biopesticide of Mirabilis jalapa and entomopathogenic fungi Beauveria bassiana to look at mortality of C. pavonana larvae observing by histological incision and scanning electron microscope. Concentration treatments of extracts M. jalapa was (control; 0,1; 0,2; 0,4 and 0,8 gr/ml) and the result showed that the effective concentration was 0,8 g/ml which affect significantly (P<0,05) in reduce pupa weight, improve pupasi time, lowering percentage of emergence imago and improve the long phase of pupa which differ significantly with control. The combination of biopesticides proved to accelerate the mortality of larvae. Histological incision observed at hour 24, 48, 72 and 96, where the biggest damage occurred at hour 96. Observation by scanning electron microscope showed fungus spores that attach to the body surface of larvae subsequently penetrate into the body. Thus the combination use of biopesticides M. jalapa and fungi B. bassiana, can be used as an alternative pest control C. pavonana.

  4. Pseudomonas aeruginosa Population Structure Revisited

    PubMed Central

    Pirnay, Jean-Paul; Bilocq, Florence; Pot, Bruno; Cornelis, Pierre; Zizi, Martin; Van Eldere, Johan; Deschaght, Pieter; Vaneechoutte, Mario; Jennes, Serge; Pitt, Tyrone; De Vos, Daniel

    2009-01-01

    At present there are strong indications that Pseudomonas aeruginosa exhibits an epidemic population structure; clinical isolates are indistinguishable from environmental isolates, and they do not exhibit a specific (disease) habitat selection. However, some important issues, such as the worldwide emergence of highly transmissible P. aeruginosa clones among cystic fibrosis (CF) patients and the spread and persistence of multidrug resistant (MDR) strains in hospital wards with high antibiotic pressure, remain contentious. To further investigate the population structure of P. aeruginosa, eight parameters were analyzed and combined for 328 unrelated isolates, collected over the last 125 years from 69 localities in 30 countries on five continents, from diverse clinical (human and animal) and environmental habitats. The analysed parameters were: i) O serotype, ii) Fluorescent Amplified-Fragment Length Polymorphism (FALFP) pattern, nucleotide sequences of outer membrane protein genes, iii) oprI, iv) oprL, v) oprD, vi) pyoverdine receptor gene profile (fpvA type and fpvB prevalence), and prevalence of vii) exoenzyme genes exoS and exoU and viii) group I pilin glycosyltransferase gene tfpO. These traits were combined and analysed using biological data analysis software and visualized in the form of a minimum spanning tree (MST). We revealed a network of relationships between all analyzed parameters and non-congruence between experiments. At the same time we observed several conserved clones, characterized by an almost identical data set. These observations confirm the nonclonal epidemic population structure of P. aeruginosa, a superficially clonal structure with frequent recombinations, in which occasionally highly successful epidemic clones arise. One of these clones is the renown and widespread MDR serotype O12 clone. On the other hand, we found no evidence for a widespread CF transmissible clone. All but one of the 43 analysed CF strains belonged to a ubiquitous P. aeruginosa “core lineage” and typically exhibited the exoS+/exoU? genotype and group B oprL and oprD alleles. This is to our knowledge the first report of an MST analysis conducted on a polyphasic data set. PMID:19936230

  5. 21 CFR 520.90d - Ampicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...of respiratory tract infections (tracheobronchitis...Escherichia coli, Pseudomonas spp., Proteus...spp., urinary tract infections (cystitis) due to...coli ; generalized infections (septicemia) associated...Proteus spp., and Pseudomonas spp. (iii)...

  6. ECF Sigma Factor regulation in Pseudomonas syringae

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomanads are renowned for their capacity to adapt to diverse environments, a fact that is reflected by the proportion of their genomes dedicated to encoding transcription regulators. Members of the Pseudomonas genus include species that are adapted to pathogenic and symbiotic lifestyles in asso...

  7. Chemotaxis of Pseudomonas putida toward chlorinated benzoates

    SciTech Connect

    Harwood, C.S.; Parales, R.E.; Dispensa, M. )

    1990-05-01

    The chlorinated aromatic acids 3-chlorobenzoate and 4-chlorobenzoate are chemoattractants for Pseudomonas putida PRS2000. These compounds are detected by a chromosomally encoded chemotactic response to benzoate which is inducible by {beta}-ketoadipate, and intermediate of benzoate catabolism. Plasmid pAC27, encoding enzymes for 3-chlorobenzoate degradation, does not appear to carry genes for chemotaxis toward chlorinated compounds.

  8. Pseudomonas fluorescens -A robust manufacturing platform

    E-print Network

    Lebendiker, Mario

    Pseudomonas fluorescens -A robust manufacturing platform Reprinted from July/August 2004 Speciality at efficient- ly transporting single chain antibodies and other mammalian-derived proteins. In addition production. Dowpharma, a contract manufacturing services unit of Dow Chemical, has developed a manufacturing

  9. LWR-PROTEUS Verification of Reaction Rate Distributions in Modern 10 x 10 Boiling Water Reactor Fuel

    SciTech Connect

    Jatuff, F.; Grimm, P.; Joneja, O.; Murphy, M.; Luethi, A.; Seiler, R.; Brogli, R.; Jacot-Guillarmod, R.; Williams, T.; Helmersson, S.; Chawla, R.

    2001-11-15

    HELIOS, CASMO-4, and MCNP4B calculations of reaction rate distributions in a modern, fresh 10 x 10 boiling water reactor fuel element have been validated using the experimental results of the LWR-PROTEUS Phase I project corresponding to full-density water moderation conditions (core 1B). The reaction rate distributions measured with a special gamma-scanning machine employing twin germanium detectors consisted of total fission F{sub tot} and {sup 238}U-capture C{sub 8}. The average statistical errors for the gamma scans were better than 0.5% for F{sub tot} and 0.9% for C{sub 8}. The rod-by-rod measurements were performed on 60 different fuel rods selected from the central part of a test zone consisting of actual, fresh SVEA-96+ fuel elements, thus gaining in realism by departing from conventional fuel rod mockups. In the case of F{sub tot}, the root-mean-square (rms) of the rod-by-rod distribution of differences between calculational and experimental (C-E) values has been found to be 1.1% for HELIOS and for CASMO-4, and 1.3% for MCNP4B. For C{sub 8}, the rms values of the (C-E) distributions are 1.0, 1.3, and 1.4% as obtained with HELIOS, CASMO-4, and MCNP4B, respectively. The effects of using different data libraries (ENDF/B-V, ENDF/B-VI, and JEF-2.2) with MCNP4B were also studied and have been found to be small.

  10. HTR-PROTEUS Pebble Bed Experimental Program Cores 1, 1A, 2, and 3: Hexagonal Close Packing with a 1:2 Moderator-to-Fuel Pebble Ratio

    SciTech Connect

    John D. Bess; Barbara H. Dolphin; James W. Sterbentz; Luka Snoj; Igor Lengar; Oliver Köberl

    2013-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. Four benchmark experiments were evaluated in this report: Cores 1, 1A, 2, and 3. These core configurations represent the hexagonal close packing (HCP) configurations of the HTR-PROTEUS experiment with a moderator-to-fuel pebble ratio of 1:2. Core 1 represents the only configuration utilizing ZEBRA control rods. Cores 1A, 2, and 3 use withdrawable, hollow, stainless steel control rods. Cores 1 and 1A are similar except for the use of different control rods; Core 1A also has one less layer of pebbles (21 layers instead of 22). Core 2 retains the first 16 layers of pebbles from Cores 1 and 1A and has 16 layers of moderator pebbles stacked above the fueled layers. Core 3 retains the first 17 layers of pebbles but has polyethylene rods inserted between pebbles to simulate water ingress. The additional partial pebble layer (layer 18) for Core 3 was not included as it was used for core operations and not the reported critical configuration. Cores 1, 1A, 2, and 3 were determined to be acceptable benchmark experiments.

  11. HTR-PROTEUS Pebble Bed Experimental Program Cores 1, 1A, 2, and 3: Hexagonal Close Packing with a 1:2 Moderator-to-Fuel Pebble Ratio

    SciTech Connect

    John D. Bess; Barbara H. Dolphin; James W. Sterbentz; Luka Snoj; Igor Lengar; Oliver Köberl

    2012-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. Four benchmark experiments were evaluated in this report: Cores 1, 1A, 2, and 3. These core configurations represent the hexagonal close packing (HCP) configurations of the HTR-PROTEUS experiment with a moderator-to-fuel pebble ratio of 1:2. Core 1 represents the only configuration utilizing ZEBRA control rods. Cores 1A, 2, and 3 use withdrawable, hollow, stainless steel control rods. Cores 1 and 1A are similar except for the use of different control rods; Core 1A also has one less layer of pebbles (21 layers instead of 22). Core 2 retains the first 16 layers of pebbles from Cores 1 and 1A and has 16 layers of moderator pebbles stacked above the fueled layers. Core 3 retains the first 17 layers of pebbles but has polyethylene rods inserted between pebbles to simulate water ingress. The additional partial pebble layer (layer 18) for Core 3 was not included as it was used for core operations and not the reported critical configuration. Cores 1, 1A, 2, and 3 were determined to be acceptable benchmark experiments.

  12. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORE 4: RANDOM PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    SciTech Connect

    John D. Bess; Leland M. Montierth

    2013-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. One benchmark experiment was evaluated in this report: Core 4. Core 4 represents the only configuration with random pebble packing in the HTR-PROTEUS series of experiments, and has a moderator-to-fuel pebble ratio of 1:1. Three random configurations were performed. The initial configuration, Core 4.1, was rejected because the method for pebble loading, separate delivery tubes for the moderator and fuel pebbles, may not have been completely random; this core loading was rejected by the experimenters. Cores 4.2 and 4.3 were loaded using a single delivery tube, eliminating the possibility for systematic ordering effects. The second and third cores differed slightly in the quantity of pebbles loaded (40 each of moderator and fuel pebbles), stacked height of the pebbles in the core cavity (0.02 m), withdrawn distance of the stainless steel control rods (20 mm), and withdrawn distance of the autorod (30 mm). The 34 coolant channels in the upper axial reflector and the 33 coolant channels in the lower axial reflector were open. Additionally, the axial graphite fillers used in all other HTR-PROTEUS configurations to create a 12-sided core cavity were not used in the randomly packed cores. Instead, graphite fillers were placed on the cavity floor, creating a funnel-like base, to discourage ordering effects during pebble loading. Core 4 was determined to be acceptable benchmark experiment.

  13. HTR-PROTEUS PEBBLE BED EXPERIMENTAL PROGRAM CORE 4: RANDOM PACKING WITH A 1:1 MODERATOR-TO-FUEL PEBBLE RATIO

    SciTech Connect

    John D. Bess; Leland M. Montierth

    2014-03-01

    In its deployment as a pebble bed reactor (PBR) critical facility from 1992 to 1996, the PROTEUS facility was designated as HTR-PROTEUS. This experimental program was performed as part of an International Atomic Energy Agency (IAEA) Coordinated Research Project (CRP) on the Validation of Safety Related Physics Calculations for Low Enriched HTGRs. Within this project, critical experiments were conducted for graphite moderated LEU systems to determine core reactivity, flux and power profiles, reaction-rate ratios, the worth of control rods, both in-core and reflector based, the worth of burnable poisons, kinetic parameters, and the effects of moisture ingress on these parameters. One benchmark experiment was evaluated in this report: Core 4. Core 4 represents the only configuration with random pebble packing in the HTR-PROTEUS series of experiments, and has a moderator-to-fuel pebble ratio of 1:1. Three random configurations were performed. The initial configuration, Core 4.1, was rejected because the method for pebble loading, separate delivery tubes for the moderator and fuel pebbles, may not have been completely random; this core loading was rejected by the experimenters. Cores 4.2 and 4.3 were loaded using a single delivery tube, eliminating the possibility for systematic ordering effects. The second and third cores differed slightly in the quantity of pebbles loaded (40 each of moderator and fuel pebbles), stacked height of the pebbles in the core cavity (0.02 m), withdrawn distance of the stainless steel control rods (20 mm), and withdrawn distance of the autorod (30 mm). The 34 coolant channels in the upper axial reflector and the 33 coolant channels in the lower axial reflector were open. Additionally, the axial graphite fillers used in all other HTR-PROTEUS configurations to create a 12-sided core cavity were not used in the randomly packed cores. Instead, graphite fillers were placed on the cavity floor, creating a funnel-like base, to discourage ordering effects during pebble loading. Core 4 was determined to be acceptable benchmark experiment.

  14. The effect of Mirabilis jalapa leaves biopesticide treatment on the mycelium growth of entomopathogenic fungi Beauveria bassiana inside the larvae body Crocidolomia binotalis

    NASA Astrophysics Data System (ADS)

    Pramita, Mia; Anggraeni, Tjandra

    2015-09-01

    Pest control with biological method (biopesticide and entomopathogenic fungi) is an alternative program to reduce application of chemical insecticide. Biopesticide of Mirabilis jalapa leaves has been discovered rich in secondary metabolites which has antifeedant activity that can provide physiological interference in insect larvae and the generation numbers[1]. Entomopathogenic fungi Beauveria bassiana has potential to control pest populations[2]. The growth of mycelium B. bassiana may interfere metabolism process inside the host body. Otherwise, B. bassiana produce toxins such as beauvericin that can increase mortality of pest. Combination of M. jalapa and B. bassiana reduce LT50 on C. binotalis larvae[3]. Thus, this study aims to determine influence of provision of biopesticide M. jalapa leaves on growth of mycelium entomopathogenic fungi B. bassiana inside larvae body C. binotalis and to detect the presence of beauvericin in vivo. Third instar larvae of C. binotalis were divided into a control, fungal and combination group. The combination group was given biopesticide and fungi. The concentration of biopesticide was 0.8% (w/v) and concentration of fungi spores was 107 spores/ml. Spores (vol. 5µl) done topically to larvae in interval 6 hours after treatment of biopesticide on non-pesticide cabbage leaves. Afterwards, histological observations performed at 24, 48, 72, 96 hours after treatment. The result show of emergence hyphae and mycelium growth inside lumen of larvae midgut on combination group faster than fungal group. This is thought to be caused by the influence of secondary metabolites of biopesticide M. jalapa leaves. In addition, beauviricin is detectable both of fungal and combination group. Thus, it can be concluded that treatment of biopesticide from M. jalapa leaves can accelerate on growth of mycelium entomopathogenic fungi B. bassiana inside the larvae body C. binotalis and toxic of B. bassiana such as beauvericin was detected on fungal and combination group.

  15. Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii.

    PubMed

    Tvrzová, Ludmila; Schumann, Peter; Spröer, Cathrin; Sedlácek, Ivo; Pácová, Zdena; Sedo, Ondrej; Zdráhal, Zbynek; Steffen, Maike; Lang, Elke

    2006-11-01

    Two strains of Gram-negative bacteria isolated from soil by selective enrichment with nitroaromatics were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence analysis, the two strains were found to belong to the genus Pseudomonas, within the Gammaproteobacteria. Strain 1B4T shared the highest sequence similarity with Pseudomonas koreensis DSM 16610T (99.5%) and Pseudomonas jessenii CCM 4840T (99.3%), and strain 2B2T with Pseudomonas asplenii DSM 17133T (98.9%), Pseudomonas fuscovaginae DSM 7231T (98.9%) and Pseudomonas putida DSM 291T (98.7%). On the basis of phylogenetic analysis, DNA-DNA hybridization and phenotype, including chemotaxonomic characteristics, two novel species, Pseudomonas moraviensis sp. nov. with the type strain 1B4T (=CCM 7280T=DSM 16007T) and Pseudomonas vranovensis sp. nov. with the type strain 2B2T (=CCM 7279T=DSM 16006T), are proposed. The description of P. asplenii was emended on the basis of additional data obtained in this study. PMID:17082407

  16. SHORT COMMUNICATION Gallium induces the production of virulence factors in Pseudomonas

    E-print Network

    Wood, Thomas K.

    SHORT COMMUNICATION Gallium induces the production of virulence factors in Pseudomonas aeruginosa the authors show that gallium, which has antimicrobial properties, interferes with iron homeostasis highly relevant for treatment of Pseudomonas infections. Keywords Pseudomonas aeruginosa; gallium nitrate

  17. Pseudomonas aeruginosa forms Biofilms in Acute Infection Independent of Cell-to-Cell Signaling

    E-print Network

    2006-01-01

    Pseudomonas aeruginosa forms Biofilms in Acute InfectionPseudomonas aeruginosa forms biofilms within 8 hours of infectionPseudomonas and Aeromonas species and clinical diagnosis of P. aeruginosa infection

  18. Aluminum detoxification in Pseudomonas fluorescens is mediated by oxalate and phosphatidylethanolamine

    E-print Network

    Appanna, Vasu

    Aluminum detoxification in Pseudomonas fluorescens is mediated by oxalate carbon source to the soil microbe Pseudomonas fluorescens. We have demonstrated the ability September 2002; accepted 23 September 2002 Abstract 13 C NMR studies with aluminum (Al)-stressed Pseudomonas

  19. Enhanced annotations and features for comparing thousands of Pseudomonas genomes in the Pseudomonas genome database

    PubMed Central

    Winsor, Geoffrey L.; Griffiths, Emma J.; Lo, Raymond; Dhillon, Bhavjinder K.; Shay, Julie A.; Brinkman, Fiona S. L.

    2016-01-01

    The Pseudomonas Genome Database (http://www.pseudomonas.com) is well known for the application of community-based annotation approaches for producing a high-quality Pseudomonas aeruginosa PAO1 genome annotation, and facilitating whole-genome comparative analyses with other Pseudomonas strains. To aid analysis of potentially thousands of complete and draft genome assemblies, this database and analysis platform was upgraded to integrate curated genome annotations and isolate metadata with enhanced tools for larger scale comparative analysis and visualization. Manually curated gene annotations are supplemented with improved computational analyses that help identify putative drug targets and vaccine candidates or assist with evolutionary studies by identifying orthologs, pathogen-associated genes and genomic islands. The database schema has been updated to integrate isolate metadata that will facilitate more powerful analysis of genomes across datasets in the future. We continue to place an emphasis on providing high-quality updates to gene annotations through regular review of the scientific literature and using community-based approaches including a major new Pseudomonas community initiative for the assignment of high-quality gene ontology terms to genes. As we further expand from thousands of genomes, we plan to provide enhancements that will aid data visualization and analysis arising from whole-genome comparative studies including more pan-genome and population-based approaches. PMID:26578582

  20. GlpC gene is responsible for biofilm formation and defense against phagocytes and imparts tolerance to pH and organic solvents in Proteus vulgaris.

    PubMed

    Wu, Y L; Liu, K S; Yin, X T; Fei, R M

    2015-01-01

    Biofilm-forming bacteria are highly resistant to antibiotics, host immune defenses, and other external conditions. The formation of biofilms plays a key role in colonization and infection. To explore the mechanism of biofilm formation, mutant strains of Proteus vulgaris XC 2 were generated by Tn5 random transposon insertion. Only one biofilm defective bacterial species was identified from among 500 mutants. Inactivation of the glpC gene coding an anaerobic glycerol-3-phosphate dehydrogenase subunit C was identified by sequence analysis of the biofilm defective strain. Differences were detected in the growth phenotypes of the wild-type and mutant strains under pH, antibiotic, and organic solvent stress conditions. Furthermore, we observed an increase in the phagocytosis of the biofilm defective strain by the mouse macrophage RAW264.7 cell line compared to the wild-type strain. This study shows that the glpC gene plays an important role in biofilm formation, in addition to imparting pH, organic solvent, and antibiotic tolerance, and defense against phagocytosis to Proteus sp. The results further clarified the mechanism of biofilm formation at the genomic level, and indicated the importance of the glpC gene in this process. This data may provide innovative therapeutic measures against P. vulgaris infections; furthermore, as an important crocodile pathogen, this study also has important significance in the protection of Chinese alligators. PMID:26400293

  1. In Vitro Cytotoxic Effects of Gold Nanoparticles Coated with Functional Acyl Homoserine Lactone Lactonase Protein from Bacillus licheniformis and Their Antibiofilm Activity against Proteus Species

    PubMed Central

    Vinoj, Gopalakrishnan; Pati, Rashmirekha; Sonawane, Avinash

    2014-01-01

    N-acylated homoserine lactonases are known to inhibit the signaling molecules of the biofilm-forming pathogens. In this study, gold nanoparticles were coated with N-acylated homoserine lactonase proteins (AiiA AuNPs) purified from Bacillus licheniformis. The AiiA AuNPs were characterized by UV-visible spectra, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and X-ray diffraction (XRD). The synthesized AiiA AuNPs were found to be spherical in shape and 10 to 30 nm in size. Treatment with AiiA protein-coated AuNPs showed maximum reduction in exopolysaccharide production, metabolic activities, and cell surface hydrophobicity and potent antibiofilm activity against multidrug-resistant Proteus species compared to treatment with AiiA protein alone. AiiA AuNPs exhibited potent antibiofilm activity at 2 to 8 ?M concentrations without being harmful to the macrophages. We conclude that at a specific dose, AuNPs coated with AiiA can kill bacteria without harming the host cells, thus representing a potential template for the design of novel antibiofilm and antibacterial protein drugs to decrease bacterial colonization and to overcome the problem of drug resistance. In summary, our data suggest that the combined effect of the lactonase and the gold nanoparticles of the AiiA AuNPs has promising antibiofilm activity against biofilm-forming and multidrug-resistant Proteus species. PMID:25403677

  2. Lung abscess due to Pseudomonas cepacia.

    PubMed

    Poe, R H; Marcus, H R; Emerson, G L

    1977-05-01

    A diabetic patient with pneumonia of unspecified origin developed a lung abscess after therapy with ultrasonic nebulization. The etiologic organism was identified as Pseudomonas cepacia. Investigation determined the source of the organism to be the reservoir of the ultrasonic nebulizer, to which the patient was directly exposed through removal of the bottom of the disposable medication cup. When this organism is isolated a nosocomial source of infection should be suspected. PMID:855960

  3. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ...abscesses, and meningitis (inflammation of brain membranes). Pseudomonas pseudomallei causes melioidosis, a chronic pneumonia. (b) Classification. Class II (special controls). The device is exempt from the premarket notification...

  4. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...abscesses, and meningitis (inflammation of brain membranes). Pseudomonas pseudomallei causes melioidosis, a chronic pneumonia. (b) Classification. Class II (special controls). The device is exempt from the premarket notification...

  5. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ...abscesses, and meningitis (inflammation of brain membranes). Pseudomonas pseudomallei causes melioidosis, a chronic pneumonia. (b) Classification. Class II (special controls). The device is exempt from the premarket notification...

  6. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...abscesses, and meningitis (inflammation of brain membranes). Pseudomonas pseudomallei causes melioidosis, a chronic pneumonia. (b) Classification. Class II (special controls). The device is exempt from the premarket notification...

  7. Pseudomonas taiwanensis sp. nov., isolated from soil.

    PubMed

    Wang, Li-Ting; Tai, Chun-Ju; Wu, Yen-Chi; Chen, Ying-Bei; Lee, Fwu-Ling; Wang, San-Lang

    2010-09-01

    A novel Gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, CMS(T), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16S rRNA gene sequence analysis revealed that the organism belongs phylogenetically to the genus Pseudomonas. Pseudomonas monteilii, P. plecoglossicida and P. mosselii were the most closely related species, with 16S rRNA gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. Relatively low gyrB gene sequence similarities (<90 %) and DNA-DNA reassociation values (<51 %) were obtained between the strain and its phylogenetically closest neighbours. The G+C content of strain CMS(T) was 62.7 mol%. The major cellular fatty acids were C(18 : 1) ? 7c, summed feature 3 (C(16 : 1) ? 7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(10 : 0) 3-OH. Based on the phenotypic and genetic evidence, the strain is suggested to represent a novel species, for which the name Pseudomonas taiwanensis sp. nov. is proposed. The type strain is CMS(T) (=BCRC 17751(T) =DSM 21245(T)). PMID:19854877

  8. Adhesion of Pseudomonas fluorescens onto nanophase materials

    NASA Astrophysics Data System (ADS)

    Webster, Thomas J.; Tong, Zonghua; Liu, Jin; Banks, M. Katherine

    2005-07-01

    Nanobiotechnology is a growing area of research, primarily due to the potentially numerous applications of new synthetic nanomaterials in engineering/science. Although various definitions have been given for the word 'nanomaterials' by many different experts, the commonly accepted one refers to nanomaterials as those materials which possess grains, particles, fibres, or other constituent components that have one dimension specifically less than 100 nm. In biological applications, most of the research to date has focused on the interactions between mammalian cells and synthetic nanophase surfaces for the creation of better tissue engineering materials. Although mammalian cells have shown a definite positive response to nanophase materials, information on bacterial interactions with nanophase materials remains elusive. For this reason, this study was designed to assess the adhesion of Pseudomonas fluorescens on nanophase compared to conventional grain size alumina substrates. Results provide the first evidence of increased adhesion of Pseudomonas fluorescens on alumina with nanometre compared to conventional grain sizes. To understand more about the process, polymer (specifically, poly-lactic-co-glycolic acid or PLGA) casts were made of the conventional and nanostructured alumina surfaces. Results showed similar increased Pseudomonas fluorescens capture on PLGA casts of nanostructured compared to conventional alumina as on the alumina itself. For these reasons, a key material property shown to enhance bacterial adhesion was elucidated in this study for both polymers and ceramics: nanostructured surface features.

  9. Spite and virulence in the bacterium Pseudomonas aeruginosa

    E-print Network

    Reece, Sarah

    Spite and virulence in the bacterium Pseudomonas aeruginosa R. Fredrik Inglisa,1 , Andy Gardnerb models, we carried out experiments using the bacterium Pseudomonas aeruginosa to test under what social infections. allelopathy bacteriocins disease kin selection microbial evolution In recent years there has been

  10. Genetic detection of Pseudomonas spp. in commercial Amazonian fish.

    PubMed

    Ardura, Alba; Linde, Ana R; Garcia-Vazquez, Eva

    2013-09-01

    Brazilian freshwater fish caught from large drainages like the River Amazon represent a million ton market in expansion, which is of enormous importance for export to other continents as exotic seafood. A guarantee of bacteriological safety is required for international exports that comprise a set of different bacteria but not any Pseudomonas. However, diarrhoea, infections and even septicaemia caused by some Pseudomonas species have been reported, especially in immune-depressed patients. In this work we have employed PCR-based methodology for identifying Pseudomonas species in commercial fish caught from two different areas within the Amazon basin. Most fish caught from the downstream tributary River Tapajňs were contaminated by five different Pseudomonas species. All fish samples obtained from the River Negro tributary (Manaus markets) contained Pseudomonas, but a less diverse community with only two species. The most dangerous Pseudomonas species for human health, P. aeruginosa, was not found and consumption of these fish (from their Pseudomonas content) can be considered safe for healthy consumers. As a precautionary approach we suggest considering Pseudomonas in routine bacteriological surveys of imported seafood. PMID:24065035

  11. Pseudomonas Exotoxin A: optimized by evolution for effective killing

    PubMed Central

    Michalska, Marta; Wolf, Philipp

    2015-01-01

    Pseudomonas Exotoxin A (PE) is the most toxic virulence factor of the pathogenic bacterium Pseudomonas aeruginosa. This review describes current knowledge about the intoxication pathways of PE. Moreover, PE represents a remarkable example for pathoadaptive evolution, how bacterial molecules have been structurally and functionally optimized under evolutionary pressure to effectively impair and kill their host cells. PMID:26441897

  12. Genomics of Secondary Metabolite Production by Pseudomonas fluorescens Pf-5

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas spp. are prolific producers of secondary metabolites, and the availability of genomic sequences now opens the door for discovery of novel natural products with potential roles in the ecology and plant growth promoting properties of these bacteria. The rhizosphere bacterium Pseudomonas f...

  13. Genetic Detection of Pseudomonas spp. in Commercial Amazonian Fish

    PubMed Central

    Ardura, Alba; Linde, Ana R.; Garcia-Vazquez, Eva

    2013-01-01

    Brazilian freshwater fish caught from large drainages like the River Amazon represent a million ton market in expansion, which is of enormous importance for export to other continents as exotic seafood. A guarantee of bacteriological safety is required for international exports that comprise a set of different bacteria but not any Pseudomonas. However, diarrhoea, infections and even septicaemia caused by some Pseudomonas species have been reported, especially in immune-depressed patients. In this work we have employed PCR-based methodology for identifying Pseudomonas species in commercial fish caught from two different areas within the Amazon basin. Most fish caught from the downstream tributary River Tapajňs were contaminated by five different Pseudomonas species. All fish samples obtained from the River Negro tributary (Manaus markets) contained Pseudomonas, but a less diverse community with only two species. The most dangerous Pseudomonas species for human health, P. aeruginosa, was not found and consumption of these fish (from their Pseudomonas content) can be considered safe for healthy consumers. As a precautionary approach we suggest considering Pseudomonas in routine bacteriological surveys of imported seafood. PMID:24065035

  14. Recombineering using RecET from Pseudomonas syringae

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Here we report the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecE...

  15. Protein Engineering of Toluene 4-Monooxygenase of Pseudomonas

    E-print Network

    Wood, Thomas K.

    at 200 AM nitrobenzene (0.13 F 0.01 vs. 0.008 F 0.001 nmol/minÁmg protein). HPLC and mass spectrometryProtein Engineering of Toluene 4-Monooxygenase of Pseudomonas mendocina KR1 for Synthesizing 4 Abstract: After discovering that toluene 4-monooxygen- ase (T4MO) of Pseudomonas mendocina KR1 oxidizes

  16. 21 CFR 520.1618 - Orbifloxacin suspension.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... pseudintermedius, Proteus mirabilis, Escherichia coli, and Enterococcus faecalis and skin and soft tissue... spp., Klebsiella pneumoniae, E. coli, Enterobacter spp., Citrobacter spp., E. faecalis, ?-hemolytic...) in cats caused by susceptible strains of S. aureus, E. coli, and P. multocida....

  17. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., tonsillitis, and bronchitis due to Streptococcus spp., Staphylococcus spp., Escherichia coli, Proteus...., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus spp.; gastrointestinal infections due to Staphylococcus spp., Streptococcus spp., Enterococcus spp., and E. coli. ; infections associated with...

  18. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., tonsillitis, and bronchitis due to Streptococcus spp., Staphylococcus spp., Escherichia coli, Proteus...., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus spp.; gastrointestinal infections due to Staphylococcus spp., Streptococcus spp., Enterococcus spp., and E. coli. ; infections associated with...

  19. 21 CFR 520.1618 - Orbifloxacin suspension.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... pseudintermedius, Proteus mirabilis, Escherichia coli, and Enterococcus faecalis and skin and soft tissue... spp., Klebsiella pneumoniae, E. coli, Enterobacter spp., Citrobacter spp., E. faecalis, ?-hemolytic...) in cats caused by susceptible strains of S. aureus, E. coli, and P. multocida....

  20. 21 CFR 520.1618 - Orbifloxacin suspension.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... pseudintermedius, Proteus mirabilis, Escherichia coli, and Enterococcus faecalis and skin and soft tissue... spp., Klebsiella pneumoniae, E. coli, Enterobacter spp., Citrobacter spp., E. faecalis, ?-hemolytic...) in cats caused by susceptible strains of S. aureus, E. coli, and P. multocida....

  1. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., tonsillitis, and bronchitis due to Streptococcus spp., Staphylococcus spp., Escherichia coli, Proteus...., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus spp.; gastrointestinal infections due to Staphylococcus spp., Streptococcus spp., Enterococcus spp., and E. coli. ; infections associated with...

  2. 21 CFR 522.313c - Ceftiofur sodium.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... mortality associated with Escherichia coli organisms susceptible to ceftiofur in day-old chicks. (6) Turkeys.... For control of early mortality associated with E. coli organisms susceptible to ceftiofur in day-old... associated with E. coli and Proteus mirabilis....

  3. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., tonsillitis, and bronchitis due to Streptococcus spp., Staphylococcus spp., Escherichia coli, Proteus...., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus spp.; gastrointestinal infections due to Staphylococcus spp., Streptococcus spp., Enterococcus spp., and E. coli. ; infections associated with...

  4. 21 CFR 522.313c - Ceftiofur sodium.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... mortality associated with Escherichia coli organisms susceptible to ceftiofur in day-old chicks. (6) Turkeys.... For control of early mortality associated with E. coli organisms susceptible to ceftiofur in day-old... associated with E. coli and Proteus mirabilis....

  5. 21 CFR 520.90b - Ampicillin trihydrate tablets.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., tonsillitis, and bronchitis due to Streptococcus spp., Staphylococcus spp., Escherichia coli, Proteus...., Staphylococcus spp., E., coli, P. mirabilis, and Enterococcus spp.; gastrointestinal infections due to Staphylococcus spp., Streptococcus spp., Enterococcus spp., and E. coli. ; infections associated with...

  6. 21 CFR 520.1618 - Orbifloxacin suspension.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... pseudintermedius, Proteus mirabilis, Escherichia coli, and Enterococcus faecalis and skin and soft tissue... spp., Klebsiella pneumoniae, E. coli, Enterobacter spp., Citrobacter spp., E. faecalis, ?-hemolytic...) in cats caused by susceptible strains of S. aureus, E. coli, and P. multocida....

  7. Biosynthesis of Gold Nanoparticles Using Pseudomonas Aeruginosa

    SciTech Connect

    Abd El-Aziz, M.; Badr, Y.; Mahmoud, M. A.

    2007-02-14

    Pseudomonas aeruginosa were used for extracellular biosynthesis of gold nanoparticles (Au NPs). Consequently, Au NPs were formed due to reduction of gold ion by bacterial cell supernatant of P. aeruginos ATCC 90271, P. aeruginos (2) and P. aeruginos (1). The UV-Vis. and fluorescence spectra of the bacterial as well as chemical prepared Au NPs were recorded. Transmission electron microscopy (TEM) micrograph showed the formation of well-dispersed gold nanoparticles in the range of 15-30 nm. The process of reduction being extracellular and may lead to the development of an easy bioprocess for synthesis of Au NPs.

  8. Binding of germanium of Pseudomonas putida cells

    SciTech Connect

    Klapcinska, B.; Chmielowski, J.

    1986-05-01

    The binding of germanium to Pseudomonas putida ATCC 33015 was investigated by using whole intact cells grown in a medium supplemented with GeO/sub 2/ and catechol or acetate. Electron-microscopic examination of the control and metal-loaded samples revealed that germanium was bound within the cell envelope. A certain number of small electron-dense deposits of the bound element were found in the cytoplasm when the cells were grown in the presence of GeO/sub 2/ and catechol. The study of germanium distribution in cellular fractions revealed that catechol facilitated the intracellular accumulation of this element.

  9. Molecular studies of Pseudomonas exotoxin A gene.

    PubMed Central

    Vasil, M L; Chamberlain, C; Grant, C C

    1986-01-01

    A 2.7-kilobase DNA fragment carrying the entire exotoxin A (ETA) structural gene was divided into three nonoverlapping probes. Two probes covering the ETA structural gene were used in colony hybridization experiments to determine whether sequences homologous to the ETA gene could be detected in genera other than Pseudomonas or in Pseudomonas species other than Pseudomonas aeruginosa. The majority of strains examined other than the P. aeruginosa strains failed to react in the colony hybridization assays. Some Pseudomonas spp. other than P. aeruginosa and some Bordetella spp. did react in colony hybridization assays with the probes. However, additional studies in which we used Southern hybridization methods indicated that these reactions were apparently nonspecific and that the ETA gene is limited to P. aeruginosa. Studies in which we used all three ETA-related probes in Southern hybridization experiments to analyze the ETA gene and surrounding sequences in P. aeruginosa strains isolated from diverse sources revealed the following: (i) the incidence of the ETA gene in P. aeruginosa is approximately equal to 95%; (ii) there are strains which have been isolated from human infections that do not carry the ETA structural gene; (iii) there is a maximum of one copy of the ETA gene per genome in any given strain; (iv) sequences within and 4 to 5 kilobases downstream of the ETA structural gene appear to be well conserved in different strains of P. aeruginosa; and (v) in contrast, sequences immediately upstream of the ETA structural gene are considerably rearranged from strain to strain. A multicopy plasmid carrying the entire cloned ETA gene was transferred to a tox- P. aeruginosa strain. This strain synthesized and secreted mature, full-length ETA, but the amount produced was small considering the multicopy nature of the plasmid. Synthesis of toxin in this strain was only minimally affected by iron. Our data suggest that the synthesis of ETA is positively regulated. Finally, we found that the presence of the ETA gene is independent of the ability of P. aeruginosa to produce several other recognized virulence factors, supporting the concept of the multifactorial nature of P. aeruginosa virulence. Images PMID:3009329

  10. Interkingdom adenosine signal reduces Pseudomonas aeruginosa pathogenicity.

    PubMed

    Sheng, Lili; Pu, Mingming; Hegde, Manjunath; Zhang, Yuanxing; Jayaraman, Arul; Wood, Thomas K

    2012-07-01

    Pseudomonas aeruginosa is becoming recognized as an important pathogen in the gastrointestinal (GI) tract. Here we demonstrate that adenosine, derived from hydrolysis of ATP from the eucaryotic host, is a potent interkingdom signal in the GI tract for this pathogen. The addition of adenosine nearly abolished P.?aeruginosa biofilm formation and abolished swarming by preventing production of rhamnolipids. Since the adenosine metabolite inosine did not affect biofilm formation and since a mutant unable to metabolize adenosine behaved like the wild-type strain, adenosine metabolism is not required to reduce pathogenicity. Adenosine also reduces production of the virulence factors pyocyanin, elastase, extracellular polysaccharide, siderophores and the Pseudomonas quinolone signal which led to reduced virulence with Caenorhabditis elegans. To provide insights into how adenosine reduces the virulence of P.?aeruginosa, a whole-transcriptome analysis was conducted which revealed that adenosine addition represses genes similar to an iron-replete condition; however, adenosine did not directly bind Fur. Therefore, adenosine decreases P.?aeruginosa pathogenicity as an interkingdom signal by causing genes related to iron acquisition to be repressed. PMID:22414222

  11. Novel Type III Effectors in Pseudomonas aeruginosa

    PubMed Central

    Burstein, David; Satanower, Shirley; Simovitch, Michal; Belnik, Yana; Zehavi, Meital; Yerushalmi, Gal; Ben-Aroya, Shay

    2015-01-01

    ABSTRACT Pseudomonas aeruginosa is a Gram-negative, opportunistic pathogen that causes chronic and acute infections in immunocompromised patients. Most P. aeruginosa strains encode an active type III secretion system (T3SS), utilized by the bacteria to deliver effector proteins from the bacterial cell directly into the cytoplasm of the host cell. Four T3SS effectors have been discovered and extensively studied in P. aeruginosa: ExoT, ExoS, ExoU, and ExoY. This is especially intriguing in light of P. aeruginosa’s ability to infect a wide range of hosts. We therefore hypothesized that additional T3SS effectors that have not yet been discovered are encoded in the genome of P. aeruginosa. Here, we applied a machine learning classification algorithm to identify novel P. aeruginosa effectors. In this approach, various types of data are integrated to differentiate effectors from the rest of the open reading frames of the bacterial genome. Due to the lack of a sufficient learning set of positive effectors, our machine learning algorithm integrated genomic information from another Pseudomonas species and utilized dozens of features accounting for various aspects of the effector coding genes and their products. Twelve top-ranking predictions were experimentally tested for T3SS-specific translocation, leading to the discovery of two novel T3SS effectors. We demonstrate that these effectors are not part of the injection structural complex and report initial efforts toward their characterization. PMID:25784698

  12. 21 CFR 520.88a - Amoxicillin trihydrate film-coated tablets.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ..., Streptococcus spp., Escherichia coli, Proteus mirabilis, and bacterial dermatitis caused by S. aureus..., Streptococcus spp., and E. coli; genitourinary tract (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract due to E. coli; and skin and soft tissue...

  13. 21 CFR 520.88a - Amoxicillin trihydrate film-coated tablets.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ..., Streptococcus spp., Escherichia coli, Proteus mirabilis, and bacterial dermatitis caused by S. aureus..., Streptococcus spp., and E. coli; genitourinary tract (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract due to E. coli; and skin and soft tissue...

  14. 21 CFR 520.88a - Amoxicillin trihydrate film-coated tablets.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ..., Streptococcus spp., Escherichia coli, Proteus mirabilis, and bacterial dermatitis caused by S. aureus..., Streptococcus spp., and E. coli; genitourinary tract (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract due to E. coli; and skin and soft tissue...

  15. 21 CFR 520.88a - Amoxicillin trihydrate film-coated tablets.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ..., Streptococcus spp., Escherichia coli, Proteus mirabilis, and bacterial dermatitis caused by S. aureus..., Streptococcus spp., and E. coli; genitourinary tract (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract due to E. coli; and skin and soft tissue...

  16. 21 CFR 520.88a - Amoxicillin trihydrate film-coated tablets.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ..., Streptococcus spp., Escherichia coli, Proteus mirabilis, and bacterial dermatitis caused by S. aureus..., Streptococcus spp., and E. coli; genitourinary tract (cystitis) due to S. aureus, Streptococcus spp., E. coli, and P. mirabilis; gastrointestinal tract due to E. coli; and skin and soft tissue...

  17. Draft Genome Sequence of Pseudomonas fluorescens LMG 5329, a White Line-Inducing Principle-Producing Bioindicator for the Mushroom Pathogen Pseudomonas tolaasii

    PubMed Central

    Rokni-Zadeh, Hassan; Zarrineh, Peyman

    2013-01-01

    Pseudomonas tolaasii, the causative agent of Agaricus bisporus brown blotch disease, can be identified by the white line reaction, occurring upon confrontation of the tolaasin-producing mushroom pathogen with “Pseudomonas reactans,” producing the lipopeptide white line-inducing principle (WLIP). The draft genome sequence of the WLIP-producing indicator Pseudomonas fluorescens strain LMG 5329 is reported here. PMID:23887909

  18. Comparative transcriptome analyses of Pseudomonas aeruginosa

    PubMed Central

    2009-01-01

    One of the hallmarks of bacterial survival is their ability to adapt rapidly to changing environmental conditions. Niche adaptation is a response to the signals received that are relayed, often to regulators that modulate gene expression. In the post-genomic era, DNA microarrays are used to study the dynamics of gene expression on a global scale. Numerous studies have used Pseudomonas aeruginosa--a Gram-negative environmental and opportunistic human pathogenic bacterium--as the model organism in whole-genome transcriptome analysis. This paper reviews the transcriptome studies that have led to immense advances in our understanding of the biology of this intractable human pathogen. Comparative analysis of 23 P. aeruginosa transcriptome studies has led to the identification of a unique set of genes that are signal specific and a core set that is differentially regulated. The 303 genes in the core set are involved in bacterial homeostasis, making them attractive therapeutic targets. PMID:19706365

  19. Mechanical robustness of Pseudomonas aeruginosa biofilms

    PubMed Central

    Lieleg, Oliver; Caldara, Marina; Baumgärtel, Regina; Ribbeck, Katharina

    2011-01-01

    Biofilms grow on various surfaces and in many different environments, a phenomenon that constitutes major problems in industry and medicine. Despite their importance little is known about the viscoelastic properties of biofilms and how these depend on the chemical microenvironment. Here, we find that the mechanical properties of Pseudomonas aeruginosa (P.a.) biofilms are highly robust towards chemical perturbations. Specifically, we observe that P.a. biofilms are able to fully regain their initial stiffness after yielding is enforced, even in the presence of chemicals. Moreover, only trivalent ions and citric acid significantly affect the biofilm elasticity, the first of which also alter the texture of the material. Finally, our results indicate that biofilm mechanics and bacteria viability inside the biofilm are not necessarily linked which suggests that targeting bacteria alone might not be sufficient for biofilm removal strategies. PMID:21760831

  20. Genetic diversity of flagellins of Pseudomonas aeruginosa.

    PubMed

    Spangenberg, C; Heuer, T; Bürger, C; Tümmler, B

    1996-11-01

    Physical genome analysis of the virulence-associated fliC locus in 20 Pseudomonas aeruginosa strains by mapping and sequencing revealed groups of heterologous a-type (1164 bp; 1185 bp) and highly conserved b-type (1467 bp) flagellin genes. Whereas only two synonymous nucleotide substitutions were detected in eight b-type fliC sequences, the 12 a-type sequences exhibited 57 nucleotide substitutions, of which 39 occurred within a variable central region. Although a-type and b-type flagellins differ by 35% in their primary structure, they share strong homology in their predicted features, implying that the polymorphic proteins fold into similar structures during polymerization of the flagella. PMID:8914989

  1. Cesium stress and adaptation in pseudomonas fluorescens

    SciTech Connect

    Appanna, V.D.; Huang, J.; St. Pierre, M.

    1996-05-01

    Industrialization and acid rain have led to a marked increment on the bioavailability of numerous metals. These metallic pollutants pose a serious threat to the ecosystem due to their ability to interact negatively with living organisms. Thus, considerable effort has been directed towards the development of environmentally-friendly technologies tailored to the management of metal wastes. As microbes are known to adapt to most environmental stresses, they constitute organisms of choice in the study of molecular adaptation processes. The adaptive features may be subsequently engineered for biotechnological applications. Cesium, a monovalent metal with chemical similarities to potassium but no know essential biological function has become a cause of environmental concern owing to its accidental release from the Chernobyl nuclear accident. This study examines the impact of cesium on the soil microbe Pseudomonas fluorescensts, and discusses the possibilities of its use in management of this nuclear waste. 15 refs., 3 figs.

  2. Pseudomonas aeruginosa endophthalmitis masquerading as chronic uveitis

    PubMed Central

    Nagaraj, Kalpana Badami; Jayadev, Chaitra

    2013-01-01

    A 65-year-old male presented with decreased vision in the left eye of 15-day duration after having undergone an uneventful cataract surgery 10 months back. He had been previously treated with systemic steroids for recurrent uveitis postoperatively on three occasions in the same eye. B-scan ultrasonography showed multiple clumplike echoes suggestive of vitreous inflammation. Aqueous tap revealed Pseudomonas aeruginosa sensitive to ciprofloxacin. The patient was treated with intravitreal ciprofloxacin and vancomycin along with systemic ciprofloxacin with good clinical response. Even a virulent organism such as P.aeruginosa can present as a chronic uveitis, which, if missed, can lead to a delay in accurate diagnosis and appropriate management. PMID:23803484

  3. DYNAMIC INTERACTIONS OF PSEUDOMONAS AERUGINOSA AND BACTERIOPHAGES IN LAKE WATER

    EPA Science Inventory

    The persistence and interaction between newly isolated strains of Pseudomonas aeruginosa and resident bacteriophages indigenous to a freshwater environment was monitored over 45 days in lake water microcosms. he interaction between susceptible and resistant bacteria with pure pha...

  4. Pseudomonas Folliculitis Associated with Use of Hot Tubs and Spas.

    ERIC Educational Resources Information Center

    Ramsey, Michael L.

    1989-01-01

    Discusses the history, etiology, diagnosis, histopathology, treatment, and prevention of Pseudomonas Folliculitis, an increasingly common skin infection contracted in hot tubs and, to some extent, in swimming pools. (Author/SM)

  5. New strategies for genetic engineering Pseudomonas syringae using recombination

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Here we report that DNA oligonucleotides (oligos) introduced directly into bacteria by electroporation can recombine with the bacterial chromosome. This phenomenon was identified in Pseudomonas syringae and we subsequently found that Escherichia coli, Salmonella typhimurium and Shigella flexneri are...

  6. Which microbial factors really are important in Pseudomonas aeruginosa infections?

    E-print Network

    Crousilles, Audrey; Maunders, Eve; Bartlett, Sean; Fan, Catherine; Ukor, Emem-Fong; Abdelhamid, Yassmin; Baker, Ysobel; Floto, Andres; Spring, David R.; Welch, Martin

    2015-08-19

    Over the last two decades, tens of millions of dollars have been invested in understanding virulence in the human pathogen, Pseudomonas aeruginosa. However, the top “hits” obtained in a recent TnSeq analysis aimed at identifying those genes...

  7. Draft Genome Sequence of Pseudomonas syringae pv. persicae NCPPB 2254.

    PubMed

    Zhao, Wenjun; Jiang, Hongshan; Tian, Qian; Hu, Jie

    2015-01-01

    Pseudomonas syringae pv. persicae is a pathogen that causes bacterial decline of stone fruit. Here, we report the draft genome sequence for P. syringae pv. persicae, which was isolated from Prunus persica. PMID:26044420

  8. UTILIZATION OF FLUORANTHENE BY PSEUDOMONAS PAUCIMOBILIS STRAIN EPA505

    EPA Science Inventory

    Pseudomonas paucimobilis strain EPA505, was previously purified from a 7-membered bacterial community originally isolated from a creosote-contaminated soil for its ability to degrade polycyclic aromatic hydrocarbon (PAH) components of creosote. The unique ability of this organism...

  9. Pseudomonas aeruginosa Dose-Response and Bathing Water Infection

    EPA Science Inventory

    Pseudomonas aeruginosa is the most commonly identified opportunistic pathogen associated with pool acquired bather disease. To better understand why this microorganism poses this protracted problem we recently appraised P. aeruginosa pool risk management. Much is known about the ...

  10. Spectrophotometric Ferric Ion Biosensor From Pseudomonas fluorescens Culture

    E-print Network

    Mittal, Aditya

    ARTICLE Spectrophotometric Ferric Ion Biosensor From Pseudomonas fluorescens Culture Varun Gupta Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India broth supernatant as a robust ferric ions biosensor. For characterizing the ferric ions biosensor, we

  11. Hypersusceptibility of cystic fibrosis mice to chronic Pseudomonas aeruginosa oropharyngeal

    E-print Network

    Ausubel, Frederick M.

    Hypersusceptibility of cystic fibrosis mice to chronic Pseudomonas aeruginosa oropharyngeal 02115 Contributed by Frederick M. Ausubel, December 24, 2002 No transgenic cystic fibrosis (CF) mouse lung disease. Cystic fibrosis (CF), a common and devastating human genetic disease, is caused

  12. The evolution of a pleiotropic fitness tradeoff in Pseudomonas fluorescens

    E-print Network

    Fussman, Gregor

    The evolution of a pleiotropic fitness tradeoff in Pseudomonas fluorescens R. Craig Mac that the experimental evolution of niche-specialist genotypes of the bacterium Pseudo- monas fluorescens that colonize

  13. Complete Genome Sequence of the Cyanogenic Phosphate-Solubilizing Pseudomonas sp. Strain CCOS 191, a Close Relative of Pseudomonas mosselii

    PubMed Central

    Pothier, Joël F.; Ruinelli, Michela; Blom, Jochen; Frasson, David; Koechli, Chantal; Fabbri, Carlotta; Brandl, Helmut; Duffy, Brion; Sievers, Martin

    2015-01-01

    We sequenced the complete genome of the isolate Pseudomonas sp. CCOS 191. This strain is able to dissolve phosphate minerals and form cyanide. The genome sequence is used to establish the phylogenetic relationship of this species. PMID:26067963

  14. Detection of pathogenic gram negative bacteria using infrared thermography

    NASA Astrophysics Data System (ADS)

    Lahiri, B. B.; Divya, M. P.; Bagavathiappan, S.; Thomas, Sabu; Philip, John

    2012-11-01

    Detection of viable bacteria is of prime importance in all fields of microbiology and biotechnology. Conventional methods of enumerating bacteria are often time consuming and labor-intensive. All living organisms generate heat due to metabolic activities and hence, measurement of heat energy is a viable tool for detection and quantification of bacteria. In this article, we employ a non-contact and real time method - infrared thermography (IRT) for measurement of temperature variations in four clinically significant gram negative pathogenic bacteria, viz. Vibrio cholerae, Vibrio mimicus, Proteus mirabilis and Pseudomonas aeruginosa. We observe that, the energy content, defined as the ratio of heat generated by bacterial metabolic activities to the heat lost from the liquid medium to the surrounding, vary linearly with the bacterial concentration in all the four pathogenic bacteria. The amount of energy content observed in different species is attributed to their metabolisms and morphologies that affect the convection velocity and hence heat transport in the medium.

  15. Antimicrobial activity of extracts and isoquinoline alkaloids of selected papaveraceae plants.

    PubMed

    Opletal, Lubomír; Lo?árek, Miroslav; Fra?ková, Adéla; Chlebek, Jakub; Smíd, Jakub; Hošt'álková, Anna; Safratová, Marcela; Hulcová, Daniela; Klou?ek, Pavel; Rozkot, Miroslav; Cahlíková, Lucie

    2014-12-01

    Alkaloidal extracts of seven selected plants of the family Papaveraceae were studied with respect to their activity against six strains of pathogenic bacteria and their alkaloidal fingerprint. Twenty-four alkaloids were determined by GC/MS, and twenty of them identified from their mass spectra, retention times and retention indexes. In the antibacterial assay, three Gram-positive (Enterorococcus faecalis, Staphylococcus aureus and S. hyicus), and three Gram-negative (Escherichia coli, Proteus mirabilis and Pseudomonas aeruginosa) strains were used. The most promising antimicrobial activity was shown by the alkaloidal extract of Macleaya cordata with MIC values of 16 ?g/mL for Staphylococcus aureus, 32 ?g/mL for Enterococcus faecalis and 64 ?g/mL for Staphylococcus hyicus and Escherichia coli. All the tested pure isoquinoline alkaloids were considered inactive within the tested concentrations. PMID:25632464

  16. Discrimination of selected species of pathogenic bacteria using near-infrared Raman spectroscopy and principal components analysis

    NASA Astrophysics Data System (ADS)

    de Siqueira e Oliveira, Fernanda SantAna; Giana, Hector Enrique; Silveira, Landulfo

    2012-10-01

    A method, based on Raman spectroscopy, for identification of different microorganisms involved in bacterial urinary tract infections has been proposed. Spectra were collected from different bacterial colonies (Gram-negative: Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterobacter cloacae, and Gram-positive: Staphylococcus aureus and Enterococcus spp.), grown on culture medium (agar), using a Raman spectrometer with a fiber Raman probe (830 nm). Colonies were scraped from the agar surface and placed on an aluminum foil for Raman measurements. After preprocessing, spectra were submitted to a principal component analysis and Mahalanobis distance (PCA/MD) discrimination algorithm. We found that the mean Raman spectra of different bacterial species show similar bands, and S. aureus was well characterized by strong bands related to carotenoids. PCA/MD could discriminate Gram-positive bacteria with sensitivity and specificity of 100% and Gram-negative bacteria with sensitivity ranging from 58 to 88% and specificity ranging from 87% to 99%.

  17. In vitro antibacterial, antifungal & cytotoxic activity of some isonicotinoylhydrazide Schiff's bases and their cobalt (II), copper (II), nickel (II) and zinc (II) complexes.

    PubMed

    Chohan, Zahid H; Arif, M; Shafiq, Zahid; Yaqub, Muhammad; Supuran, Claudiu T

    2006-02-01

    Isonicotinoylhydrazide Schiff's bases formed by the reaction of substituted and unsubstituted furyl-2-carboxaldehyde and thiophene-2-carboxaldehyde with isoniazid and, their Co (II), Cu (II), Ni (II) and Zn (II) complexes have been synthesized, characterized and screened for their in vitro antibacterial activity against Mycobacterium tuberculosis, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysenteriae, Bacillus cereus, Corynebacterium diphtheriae, Staphylococcus aureus and Streptococcus pyogenes bacterial strains and for in vitro antifungal activity against Trichophyton longifusus, Candida albicans, Aspergillus flavus, Microsporum canis, Fusarium solani and Candida glabrata. The results of these studies show the metal complexes to be more antibacterial and antifungal against one or more bacterial/fungal strains as compared to the uncomplexed compounds. The brine shrimp bioassay indicated Schiff's bases, L3 and L6 and, their Cu (II) and Ni (II) metal complexes to be cytotoxic against Artemia salina, while all other compounds were inactive (LD50 > 1000). PMID:16570512

  18. A new antibacterial compound produced by an indigenous marine bacteria--fermentation, isolation, and biological activity.

    PubMed

    Uzair, Bushra; Ahmed, Nuzhat; Ahmad, Viqar Uddin; Kousar, Farzana

    2006-12-01

    The use of microorganisms for biological purpose has become an effective alternative to control pathogens. A marine bacterium Pseudomonas aeruginosa was isolated from Eal fish of Baluchistan coast of Pakistan. This strain produced a bactericidal antibiotic against environmental and clinical isolates. In this study, we purified bactericidal antibiotic from the ethyl acetate extract of the cells of P. aeruginosa and analyzed its chemical structure. Based on spectrometric analysis, this compound 1 is proposed to be 1-methyl-1,4 dihydroquinoline and is active against methicillin-resistant Staphylococcus aureus (MRSA), methicillin-sensitive S. aureus (MSSA), Salmonella typhi, Shigella flexneri, Escherichia coli, Proteus mirabilis, Vibrio aliginolyticus, Micrococcus luteus, Enterococcus faecalis, Enterobacter faecium but it is not active against G streptococci, Candida albicans, Aspergillus niger. Minimal inhibitory concentration for Gram-positive bacteria was between 50 and 75 microg mL(-1) and for Gram-negative bacteria 75-100 microg mL(-1). PMID:17393659

  19. Antibacterial effects of the essential oils of commonly consumed medicinal herbs using an in vitro model.

    PubMed

    Sokovi?, Marina; Glamo?lija, Jasmina; Marin, Petar D; Brki?, Dejan; van Griensven, Leo J L D

    2010-11-01

    The chemical composition and antibacterial activity of essential oils from 10 commonly consumed herbs: Citrus aurantium, C. limon, Lavandula angustifolia, Matricaria chamomilla, Mentha piperita, M. spicata, Ocimum basilicum, Origanum vulgare, Thymus vulgaris and Salvia officinalis have been determined. The antibacterial activity of these oils and their main components; i.e. camphor, carvacrol, 1,8-cineole, linalool, linalyl acetate, limonene, menthol, a-pinene, b-pinene, and thymol were assayed against the human pathogenic bacteria Bacillus subtilis, Enterobacter cloacae, Escherichia coli O157:H7, Micrococcus flavus, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enteritidis, S. epidermidis, S. typhimurium, and Staphylococcus aureus. The highest and broadest activity was shown by O. vulgare oil. Carvacrol had the highest antibacterial activity among the tested components. PMID:21030907

  20. Pseudomonas putida and Pseudomonas fluorescens Species Group Recovery from Human Homes Varies Seasonally and by Environment

    PubMed Central

    Remold, Susanna K.; Purdy-Gibson, Megan E.; France, Michael T.; Hundley, Thomas C.

    2015-01-01

    By shedding light on variation in time as well as in space, long-term biogeographic studies can help us define organisms’ distribution patterns and understand their underlying drivers. Here we examine distributions of Pseudomonas in and around 15 human homes, focusing on the P. putida and P. fluorescens species groups. We describe recovery from 10,941 samples collected during up to 8 visits per home, occurring on average 2.6 times per year. We collected a mean of 141 samples per visit, from sites in most rooms of the house, from the surrounding yards, and from human and pet occupants. We recovered Pseudomonas in 9.7% of samples, with the majority of isolates being from the P. putida and P. fluorescens species groups (approximately 62% and 23% of Pseudomonas samples recovered respectively). Although representatives of both groups were recovered from every season, every house, and every type of environment sampled, recovery was highly variable across houses and samplings. Whereas recovery of P. putida group was higher in summer and fall than in winter and spring, P. fluorescens group isolates were most often recovered in spring. P. putida group recovery from soils was substantially higher than its recovery from all other environment types, while higher P. fluorescens group recovery from soils than from other sites was much less pronounced. Both species groups were recovered from skin and upper respiratory tract samples from healthy humans and pets, although this occurred infrequently. This study indicates that even species that are able to survive under a broad range of conditions can be rare and variable in their distributions in space and in time. For such groups, determining patterns and causes of stochastic and seasonal variability may be more important for understanding the processes driving their biogeography than the identity of the types of environments in which they can be found. PMID:26023929

  1. Pseudomonas putida and Pseudomonas fluorescens Species Group Recovery from Human Homes Varies Seasonally and by Environment.

    PubMed

    Remold, Susanna K; Purdy-Gibson, Megan E; France, Michael T; Hundley, Thomas C

    2015-01-01

    By shedding light on variation in time as well as in space, long-term biogeographic studies can help us define organisms' distribution patterns and understand their underlying drivers. Here we examine distributions of Pseudomonas in and around 15 human homes, focusing on the P. putida and P. fluorescens species groups. We describe recovery from 10,941 samples collected during up to 8 visits per home, occurring on average 2.6 times per year. We collected a mean of 141 samples per visit, from sites in most rooms of the house, from the surrounding yards, and from human and pet occupants. We recovered Pseudomonas in 9.7% of samples, with the majority of isolates being from the P. putida and P. fluorescens species groups (approximately 62% and 23% of Pseudomonas samples recovered respectively). Although representatives of both groups were recovered from every season, every house, and every type of environment sampled, recovery was highly variable across houses and samplings. Whereas recovery of P. putida group was higher in summer and fall than in winter and spring, P. fluorescens group isolates were most often recovered in spring. P. putida group recovery from soils was substantially higher than its recovery from all other environment types, while higher P. fluorescens group recovery from soils than from other sites was much less pronounced. Both species groups were recovered from skin and upper respiratory tract samples from healthy humans and pets, although this occurred infrequently. This study indicates that even species that are able to survive under a broad range of conditions can be rare and variable in their distributions in space and in time. For such groups, determining patterns and causes of stochastic and seasonal variability may be more important for understanding the processes driving their biogeography than the identity of the types of environments in which they can be found. PMID:26023929

  2. Comparative sensitivity and resistance of some strains of Pseudomonas aeruginosa and Pseudomonas stutzeri to antibacterial agents

    PubMed Central

    Russell, A. D.; Mills, A. P.

    1974-01-01

    A comparison has been made of the sensitivities to various antibiotic and non-antibiotic substances of some strains of Pseudomonas aeruginosa and P. stutzeri, the latter including strains isolated from eye and other cosmetic products and from other sources. Whereas P. aeruginosa strains showed a high resistance to cetrimide and to benzalkonium chloride, the P. stutzeri strains were generally more sensitive to these and to chlorhexidine. The P. stutzeri strains were also more sensitive to the various antibiotics tested. The loss of the ability to transfer an R factor by two strains of P. aeruginosa caused no significant change in their drug sensitivity pattern. PMID:4369876

  3. Conservation of an ATP-binding domain among recA proteins from Proteus vulgaris, erwinia carotovora, Shigella flexneri, and Escherichia coli K-12 and B/r

    SciTech Connect

    Knight, K.L.; Hess, R.M.; McEntee, K.

    1988-06-01

    The purified RecA proteins encoded by the cloned genes from Proteus vulgaris, Erwinia carotovora, Shigella flexneri, and Escherichia coli B/r were compared with the RecA protein from E. coli K-12. Each of the proteins hydrolyzed ATP in the presence of single-stranded DNA, and each was covalently modified with the photoaffinity ATP analog 8-azidoadenosine 5'-triphosphate (8N/sub 3/ATP). Two-dimensional tryptic maps of the four heterologous RecA proteins demonstrated considerable structural conservation among these bacterial genera. Moreover, when the (..cap alpha..-/sup 32/P)8N/sub 3/ATP-modified proteins were digested with trypsin and analyzed by high-performance liquid chromatography, a single peak of radioactivity was detected in each of the digests and these peptides eluted identically with the tryptic peptide T/sub 31/ of the E. coli K-12 RecA protein, which was the unique site of 8N/sub 3/ATP photolabeling. Each of the heterologous recA genes hybridized to oligonucleotide probes derived from the ATP-binding domain sequence of the E. coli K-12 gene. These last results demonstrate that the ATP-binding domain of the RecA protein has been strongly conserved for greater than 10/sup 7/ years.

  4. One-step biosynthesis of ?-ketoisocaproate from l-leucine by an Escherichia coli whole-cell biocatalyst expressing an l-amino acid deaminase from Proteus vulgaris

    PubMed Central

    Song, Yang; Li, Jianghua; Shin, Hyun-dong; Du, Guocheng; Liu, Long; Chen, Jian

    2015-01-01

    This work aimed to develop a whole-cell biotransformation process for the production of ?-ketoisocaproate from L-leucine. A recombinant Escherichia coli strain was constructed by expressing an L-amino acid deaminase from Proteus vulgaris. To enhance ?-ketoisocaproate production, the reaction conditions were optimized as follows: whole-cell biocatalyst 0.8?g/L, leucine concentration 13.1?g/L, temperature 35?°C, pH 7.5, and reaction time 20?h. Under the above conditions, the ?-ketoisocaproate titer reached 12.7?g/L with a leucine conversion rate of 97.8%. In addition, different leucine feeding strategies were examined to increase the ?-ketoisocaproate titer. When 13.1?g/L leucine was added at 2-h intervals (from 0 to 22?h, 12 addition times), the ?-ketoisocaproate titer reached 69.1?g/L, while the leucine conversion rate decreased to 50.3%. We have developed an effective process for the biotechnological production of ?-ketoisocaproate that is more environmentally friendly than the traditional petrochemical synthesis approach. PMID:26217895

  5. Results of a Neutronic Simulation of HTR-Proteus Core 4.2 using PEBBED and other INL Reactor Physics Tools: FY-09 Report

    SciTech Connect

    Hans D. Gougar

    2009-08-01

    The Idaho National Laboratory’s deterministic neutronics analysis codes and methods were applied to the computation of the core multiplication factor of the HTR-Proteus pebble bed reactor critical facility. A combination of unit cell calculations (COMBINE-PEBDAN), 1-D discrete ordinates transport (SCAMP), and nodal diffusion calculations (PEBBED) were employed to yield keff and flux profiles. Preliminary results indicate that these tools, as currently configured and used, do not yield satisfactory estimates of keff. If control rods are not modeled, these methods can deliver much better agreement with experimental core eigenvalues which suggests that development efforts should focus on modeling control rod and other absorber regions. Under some assumptions and in 1D subcore analyses, diffusion theory agrees well with transport. This suggests that developments in specific areas can produce a viable core simulation approach. Some corrections have been identified and can be further developed, specifically: treatment of the upper void region, treatment of inter-pebble streaming, and explicit (multiscale) transport modeling of TRISO fuel particles as a first step in cross section generation. Until corrections are made that yield better agreement with experiment, conclusions from core design and burnup analyses should be regarded as qualitative and not benchmark quality.

  6. Bacteriophage-Mediated Control of a Two-Species Biofilm Formed by Microorganisms Causing Catheter-Associated Urinary Tract Infections in an In Vitro Urinary Catheter Model

    PubMed Central

    Lehman, Susan M.

    2014-01-01

    Microorganisms from a patient or their environment may colonize indwelling urinary catheters, forming biofilm communities on catheter surfaces and increasing patient morbidity and mortality. This study investigated the effect of pretreating hydrogel-coated silicone catheters with mixtures of Pseudomonas aeruginosa and Proteus mirabilis bacteriophages on the development of single- and two-species biofilms in a multiday continuous-flow in vitro model using artificial urine. Novel phages were purified from sewage, characterized, and screened for their abilities to reduce biofilm development by clinical isolates of their respective hosts. Our screening data showed that artificial urine medium (AUM) is a valid substitute for human urine for the purpose of evaluating uropathogen biofilm control by these bacteriophages. Defined phage cocktails targeting P. aeruginosa and P. mirabilis were designed based on the biofilm inhibition screens. Hydrogel-coated catheters were pretreated with one or both cocktails and challenged with approximately 1 × 103 CFU/ml of the corresponding pathogen(s). The biofilm growth on the catheter surfaces in AUM was monitored over 72 to 96 h. Phage pretreatment reduced P. aeruginosa biofilm counts by 4 log10 CFU/cm2 (P ? 0.01) and P. mirabilis biofilm counts by >2 log10 CFU/cm2 (P ? 0.01) over 48 h. The presence of P. mirabilis was always associated with an increase in lumen pH from 7.5 to 9.5 and with eventual blockage of the reactor lines. The results of this study suggest that pretreatment of a hydrogel urinary catheter with a phage cocktail can significantly reduce mixed-species biofilm formation by clinically relevant bacteria. PMID:25487795

  7. Crystal Structure of the Outer Membrane Protein OpdK from Pseudomonas aeruginosa

    E-print Network

    Movileanu, Liviu

    Structure Article Crystal Structure of the Outer Membrane Protein OpdK from Pseudomonas aeruginosa in the outer membrane. Comparison of the OpdK structure with that of Pseudomonas aeruginosa OprD provides

  8. Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for

    E-print Network

    Ausubel, Frederick M.

    Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role, December 22, 2005 The opportunistic pathogen Pseudomonas aeruginosa is responsi- ble for systemic infections in immunocompromised individuals and chronic respiratory disease in patients with cystic fibrosis

  9. Full-Genome Sequence of the Plant Growth-Promoting Bacterium Pseudomonas protegens CHA0

    PubMed Central

    Schuldes, Joerg; Keel, Christoph; Maurhofer, Monika; Daniel, Rolf; Scheu, Stefan; Thuermer, Andrea

    2014-01-01

    We report the complete genome sequence of the free-living bacterium Pseudomonas protegens (formerly Pseudomonas fluorescens) CHA0, a model organism used in plant-microbe interactions, biological control of phytopathogens, and bacterial genetics. PMID:24762936

  10. Genome Sequence of Pseudomonas mendocina DLHK, Isolated from a Biotrickling Reactor

    PubMed Central

    Wong, Chi Fat; Niu, Hejingying; Jiang, Jingwei; Li, Jun; Chan, Charis May Ngor; Leung, Dennis Yiu Cheong

    2012-01-01

    Pseudomonas mendocina DLHK is an aerobic bacterium isolated from a biotrickling reactor which can remove nitric oxide, a common air pollutant from combustion exhaust gas. Here, we present the draft genome of Pseudomonas mendocina DLHK. PMID:23105066

  11. Is Glyoxylate, a Glycine-derived Ketoacid, a Potent Detoxifier of ROS in Pseudomonas fluorescens?

    E-print Network

    Appanna, Vasu

    1 Is Glyoxylate, a Glycine-derived Ketoacid, a Potent Detoxifier of ROS in Pseudomonas fluorescens as an ROS scavenger by Pseudomonas fluorescens. Metabolomic analysis revealed oxalate and formate, two

  12. Metabolic networks to combat zinc toxicity in Pseudomonas fluorescens Azhar Alhasawi

    E-print Network

    Appanna, Vasu

    Metabolic networks to combat zinc toxicity in Pseudomonas fluorescens By Azhar Alhasawi Thesis was to determine how Pseudomonas fluorescens reprograms its metabolic networks to overcome metal toxicity stemming ineffective. P. fluorescens upregulated the enzymes citrate lyase (CL), phosphoenolpyruvate carboxylase (PEPC

  13. Biochemical Adaptations in Pseudomonas fluorescens Exposed to Nitric Oxide, an Endogenous Antibacterial Agent

    E-print Network

    Appanna, Vasu

    Biochemical Adaptations in Pseudomonas fluorescens Exposed to Nitric Oxide, an Endogenous supérieures Title of Thesis Titre de la thčse BIOCHEMICAL ADAPTATIONS IN PSEUDOMONAS FLUORESCENS EXPOSED. fluorescens engineers an elaborate metabolic network to generate ATP whilst withstanding the injurious effects

  14. MEASURING THE DISPERSAL AND REENTRAINMENT OF RECOMBINANT PSEUDOMONAS SYRINGAE AT CALIFORNIA TEST SITES

    EPA Science Inventory

    The dispersal of genetically engineered Pseudomonas syringae and Pseudomonas fluorescens was investigated during and after spray applications onto plants at Brentwood and Tulelake, California. ive different sampling devices were used to evaluate the dispersal within and around te...

  15. The quinohaemoprotein lupanine hydroxylase from Pseudomonas putida.

    PubMed

    Hopper, David J; Kaderbhai, Mustak A

    2003-04-11

    Lupanine hydroxylase catalyses the first reaction in the catabolism of the alkaloid lupanine by Pseudomonas putida. It dehydrogenates the substrate, which can then be hydrated. It is a monomeric protein of M(r) 72,000 and contains a covalently bound haem and a molecule of PQQ. The gene for this enzyme has been cloned and sequenced and the derived protein sequence has a 26 amino acid signal sequence at the N-terminal for translocation of the protein to the periplasm. Many of the features seen in the sequence of lupanine hydroxylase are common with other quinoproteins including the W-motifs that are characteristic of the eight-bladed propeller structure of methanol dehydrogenase. However, the unusual disulfide bridge between adjacent cysteines that is present in some PQQ-containing enzymes is absent in lupanine hydroxylase. The C-terminal domain contains characteristics of a cytochrome c and overall the sequence shows similarities with that of the quinohaemoprotein, alcohol dehydrogenase from Comamonas testosteroni. The gene coding for lupanine hydroxylase has been successfully expressed in Escherichia coli and a procedure has been developed to renature and reactivate the enzyme, which was found to be associated with the inclusion bodies. Reactivation required addition of PQQ and was dependent on calcium ions. PMID:12686118

  16. Pseudomonas ribosomal vaccines: preparation, properties, and immunogenicity.

    PubMed Central

    Lieberman, M M

    1978-01-01

    The preparation, properties, and immunogenicity of ribosomal vaccines from Pseudomonas aeruginosa are described. These preparations, containing protein and RNA, were tested for immunogenicity by active immunization of mice and subsequent challenge with homologous, live bacteria. The results demonstrated that vaccines prepared from a majority of serotypes used were immunogenic, i.e., afforded 60 to 100% mouse protection against a challenge inoculum containing 8 to 50 50% lethal doses. In some cases vaccine doses as low as 1 microgram of RNA provided 100% mouse protection. Molecular sieve chromatography of a highly immunogenic ribosomal preparation on Sepharose 4B demonstrated the presence of two molecular weight fractions: (i) peak A, an excluded peak (thus having a molecular weight of at least 2 times 10(7)), and (ii) peak B, considerably retarded, with an elution position corresponding to a molecular weight of about 2.2 X 10(6), approximating that of typical 70S ribosomes. Both peaks A and B were immunogenic; however, the immunogenicity of peak A was greater (i.e., a smaller immunizing dose was required) than that of peak B. Peak A was shown to contain components of lipopolysaccharide in addition to protein and RNA (which comprised 80% of the dry weight of peak A). On the other hand, peak B was shown to be free of lipopolysaccharide, and 100% of its dry weight consisted of protein and RNA. PMID:101464

  17. Spaceflight promotes biofilm formation by Pseudomonas aeruginosa.

    PubMed

    Kim, Wooseong; Tengra, Farah K; Young, Zachary; Shong, Jasmine; Marchand, Nicholas; Chan, Hon Kit; Pangule, Ravindra C; Parra, Macarena; Dordick, Jonathan S; Plawsky, Joel L; Collins, Cynthia H

    2013-01-01

    Understanding the effects of spaceflight on microbial communities is crucial for the success of long-term, manned space missions. Surface-associated bacterial communities, known as biofilms, were abundant on the Mir space station and continue to be a challenge on the International Space Station. The health and safety hazards linked to the development of biofilms are of particular concern due to the suppression of immune function observed during spaceflight. While planktonic cultures of microbes have indicated that spaceflight can lead to increases in growth and virulence, the effects of spaceflight on biofilm development and physiology remain unclear. To address this issue, Pseudomonas aeruginosa was cultured during two Space Shuttle Atlantis missions: STS-132 and STS-135, and the biofilms formed during spaceflight were characterized. Spaceflight was observed to increase the number of viable cells, biofilm biomass, and thickness relative to normal gravity controls. Moreover, the biofilms formed during spaceflight exhibited a column-and-canopy structure that has not been observed on Earth. The increase in the amount of biofilms and the formation of the novel architecture during spaceflight were observed to be independent of carbon source and phosphate concentrations in the media. However, flagella-driven motility was shown to be essential for the formation of this biofilm architecture during spaceflight. These findings represent the first evidence that spaceflight affects community-level behaviors of bacteria and highlight the importance of understanding how both harmful and beneficial human-microbe interactions may be altered during spaceflight. PMID:23658630

  18. Degradation of Acetonitrile by Pseudomonas putida

    PubMed Central

    Nawaz, Mohamed S.; Chapatwala, Kirit D.; Wolfram, James H.

    1989-01-01

    A bacterium capable of utilizing high concentrations of acetonitrile as the sole source of carbon and nitrogen was isolated from soil and identified as Pseudomonas putida. This bacterium could also utilize butyronitrile, glutaronitrile, isobutyronitrile, methacrylonitrile, propionitrile, succinonitrile, valeronitrile, and some of their corresponding amides, such as acetamide, butyramide, isobutyramide, methacrylamide, propionamide, and succinamide as growth substrates. Acetonitrile-grown cells oxidized acetonitrile with a Km of 40.61 mM. Mass balance studies with [14C]acetonitrile indicated that nearly 66% of carbon of acetonitrile was released as 14CO2 and 14% was associated with the biomass. Metabolites of acetonitrile in the culture medium were acetic acid and ammonia. The acetate formed in the early stages of growth completely disappeared in the later stages. Cell extracts of acetonitrile-grown cells contained activities corresponding to nitrile hydratase and amidase, which mediate the breakdown of actonitrile into acetic acid and ammonia. Both enzymes were intracellular and inducible and hydrolyzed a wide range of substrates. The specific activity of amidase was at least 150-fold higher than the activity of the enzyme nitrile hydratase. PMID:16348008

  19. Catechol oxygenases of Pseudomonas putida mutant strains.

    PubMed Central

    Bayly, R C; McKenzie, D I

    1976-01-01

    Investigation of a mutant strain of Pseudomonas putida NCIB 10015, strain PsU-E1, showed that it had lost the ability to produce catechol 1,2-oxygenase after growth with catechol. Additional mutants of both wild-type and mutant strains PsU-E1 have been isolated that grow on catechol, but not on benzoate, yet still form a catechol 1,2-oxygenase when exposed to benzoate. These findings indicate that either there are separately induced catechol 1,2-oxygenase enzymes, or that there are two separate inducers for the one catechol 1,2-oxygenase enzyme. Comparisons of the physical properties of the catechol 1,2-oxygenases formed in response to the two different inducers show no significant differences, so it is more probable that the two proteins are the product of the same gene. Sufficient enzymes of the ortho-fission pathway are induced in the wild-type strain by the initial substrate benzoate (or an early intermediate) to commit that substrate to metabolism by ortho fission exclusively. A mechanism exists that permits metabolism of catechol by meta fission if the ortho-fission enzymes are unable to prevent its intracellular accumulation. PMID:956121

  20. Biodegradation of acyclic isoprenoids by Pseudomonas species.

    PubMed Central

    Cantwell, S G; Lau, E P; Watt, D S; Fall, R R

    1978-01-01

    The ability of various pseudomonads to utilize acyclic isoprenoids as a sole carbon source was investigated. Tests for utilization of acyclic isoprenols such as citronellol and geraniol were complicated by toxic effects of these alcohols, and most species tested were killed by exposure to citronellol or geraniol (0.1%, vol/vol) in liquid culture. In the case of Pseudomonas citronellolis, sensitivity to isoprenols is reduced by prior induction of the isoprenoid degradative pathway via either growth on succinate in the presence of citronellol or growth on citronellic acid. For this species, citronellic acid proved to be the best isoprenoid growth substrate tested. Geraniol utilization as a taxonomic indicator for different subgroups of pseudomonads is discussed. Only a few of the species tested were able to utilize acyclic isoprenoids. Two species which utilize C10 acyclic isoprenoids, P. aeruginosa and P. mendocina, were shown to contain the inducible enzyme geranyl-coenzyme A carboxylase, one of the unique enzymes in the isoprenol degradative pathway known to occur in P. citronellolis. Of the species which utilized geranitol, none showed definite growth on the homologous C15 and C20 isoprenols. PMID:681275

  1. Pseudomonas Aeruginosa: Resistance to the Max

    PubMed Central

    Poole, Keith

    2011-01-01

    Pseudomonas aeruginosa is intrinsically resistant to a variety of antimicrobials and can develop resistance during anti-pseudomonal chemotherapy both of which compromise treatment of infections caused by this organism. Resistance to multiple classes of antimicrobials (multidrug resistance) in particular is increasingly common in P. aeruginosa, with a number of reports of pan-resistant isolates treatable with a single agent, colistin. Acquired resistance in this organism is multifactorial and attributable to chromosomal mutations and the acquisition of resistance genes via horizontal gene transfer. Mutational changes impacting resistance include upregulation of multidrug efflux systems to promote antimicrobial expulsion, derepression of ampC, AmpC alterations that expand the enzyme's substrate specificity (i.e., extended-spectrum AmpC), alterations to outer membrane permeability to limit antimicrobial entry and alterations to antimicrobial targets. Acquired mechanisms contributing to resistance in P. aeruginosa include ?-lactamases, notably the extended-spectrum ?-lactamases and the carbapenemases that hydrolyze most ?-lactams, aminoglycoside-modifying enzymes, and 16S rRNA methylases that provide high-level pan-aminoglycoside resistance. The organism's propensity to grow in vivo as antimicrobial-tolerant biofilms and the occurrence of hypermutator strains that yield antimicrobial resistant mutants at higher frequency also compromise anti-pseudomonal chemotherapy. With limited therapeutic options and increasing resistance will the untreatable P. aeruginosa infection soon be upon us? PMID:21747788

  2. Pseudomonas aeruginosa Induced Lung Injury Model

    PubMed Central

    Suresh Kumar, Varsha; Sadikot, Ruxana T.; Purcell, Jeanette E.; Malik, Asrar B.; Liu, Yuru

    2014-01-01

    In order to study human acute lung injury and pneumonia, it is important to develop animal models to mimic various pathological features of this disease. Here we have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa (P. aeruginosa or PA). Using this model, we were able to show lung inflammation at the early phase of injury. In addition, alveolar epithelial barrier leakiness was observed by analyzing bronchoalveolar lavage (BAL); and alveolar cell death was observed by Tunel assay using tissue prepared from injured lungs. At a later phase following injury, we observed cell proliferation required for the repair process. The injury was resolved 7 days from the initiation of P. aeruginosa injection. This model mimics the sequential course of lung inflammation, injury and repair during pneumonia. This clinically relevant animal model is suitable for studying pathology, mechanism of repair, following acute lung injury, and also can be used to test potential therapeutic agents for this disease. PMID:25406628

  3. Nitrite inhibition of denitrification by Pseudomonas fluorescens

    SciTech Connect

    Almeida, J.S.; Julio, S.M.; Reis, M.A.M. |

    1995-05-05

    Using a pure culture of Pseudomonas fluorescens as a model system nitrite inhibition of denitrification was studied. A mineral media with acetate and nitrate as sole electron donor and acceptor, respectively, was used. Results obtained in continuous stirred-tank reactors (CSTR) operated at pH values between 6.6 and 7.8 showed that growth inhibition depended only on the nitrite undissociated fraction concentration (nitrous acid). A mathematical model to describe this dependence is put forward. The maximum nitrous acid concentration compatible with cell growth and denitrification activity was found to be 66 {mu}g N/L. Denitrification activity was partially associated with growth, as described by the Luedeking-Piret equation. However, when the freshly inoculated reactor was operated discontinuously, nitrite accumulation caused growth uncoupling from denitrification activity. The authors suggest that these results can be interpreted considering that (a) nitrous acid acts as a proton uncoupler; and (b) cultures continuously exposed to nitrous acid prevent the uncoupling effect but not the growth inhibition. Examination of the growth dependence on nitrite concentration at pH 7.0 showed that adapted cultures (growth on CSTR) are less sensitive to nitrous acid inhibition than the ones cultivated in batch.

  4. Dynamics of phenol degradation by Pseudomonas putida

    SciTech Connect

    Allsop, P.J.; Chisti, Y.; Mooyoung, M.; Sullivan, G.R. )

    1993-03-05

    Pure cultures of Pseudomonas putida (ATCC 17484) were grown in continuous culture on phenol at dilution rates of 0.074-0.085 h[sup [minus]1] and subjected to step increases in phenol feed concentration. Three distinct patterns of dynamic response were obtained depending on the size of the step change used: low level, moderate level, or high level. During low level responses no accumulations of phenol or non-phenol, non-glucose-dissolved organic carbon, DOC(NGP), were observed. Moderate level responses were characterized by the transient accumulation of DOC(NGP) with a significant delay prior to phenol leakage. High level responses demonstrated a rapid onset of phenol leakage and no apparent accumulations of DOC(NGP). The addition of phenol to a continuous culture of the same organism on glucose did not result in transient DOC(NGP) accumulations, although transient phenol levels exceeded 90 mg L[sup [minus]1]. These results were consistent with intermediate metabolite production during phenol step tests coupled with substrate-inhibited phenol uptake and suggested that traditional kinetic models based on the Haldane equation may be inadequate for describing the dynamics of phenol degrading systems.

  5. Increased Sheep Lung Vascular Permeability Caused by Pseudomonas Bacteremia

    PubMed Central

    Brigham, Kenneth L.; Woolverton, William C.; Blake, Lynn H.; Staub, Norman C.

    1974-01-01

    In awake sheep, we compared the responses of lung lymph flow and lymph and plasma protein concentrations to steady state elevations of pulmonary vascular pressures made by inflating a left atrial balloon with those after an intravenous infusion of 105-1010Pseudomonas aeruginosa. Lymph flow increased when pressure was increased, but lymph-plasma protein concentration ratios always fell and lymph protein flow (lymph flow × lymph protein concentration) increased only slightly. After Pseudomonas, sheep had transient chills, fever, leukopenia, hypoxemia, increased pulmonary artery pressure and lymph flow and decreased left atrial pressure and lymph protein concentration, 3-5 h after Pseudomonas, when vascular pressures and lymph protein concentrations had returned to near base line, lymph flow increased further to 3-10 times base line and remained at a steady level for many hours. During this steady state period, lymph-plasma protein concentration ratios were similar to base line and lymph protein flow was higher than in the increased pressure studies. Two sheep died of pulmonary edema 7 and 9 h after Pseudomonas, but in 16 studies, five other sheep appeared well during the period of highest lymph flow and all variables returned to base line in 24-72 h. Six serial indicator dilution lung water studies in five sheep changed insignificantly from base line after Pseudomonas. Postmortem lung water was high in the two sheep dead of pulmonary edema and one other, but six sheep killed 1-6 h after Pseudomonas had normal lung water. Because of the clear difference between the effects of increased pressure and Pseudomonas on lymphplasma protein concentration ratios and lymph protein flow, we conclude that Pseudomonas causes a prolonged increase in lung vessel permeability to protein. Because we saw lung lymph flow as high as 10 times base line without pulmonary edema, we conclude that lung lymphatics are a sensitive high-capacity mechanism for removing excess filtered fluid. An equivalent pore model of sheep lung vessels suggests that the changes we saw after Pseudomonas could result from small changes in the structure of exchanging vessel walls. Images PMID:4430713

  6. Efflux as a Glutaraldehyde Resistance Mechanism in Pseudomonas fluorescens and Pseudomonas aeruginosa Biofilms

    PubMed Central

    Vikram, Amit; Bomberger, Jennifer M.

    2015-01-01

    A major challenge in microbial biofilm control is biocide resistance. Phenotypic adaptations and physical protective effects have been historically thought to be the primary mechanisms for glutaraldehyde resistance in bacterial biofilms. Recent studies indicate the presence of genetic mechanisms for glutaraldehyde resistance, but very little is known about the contributory genetic factors. Here, we demonstrate that efflux pumps contribute to glutaraldehyde resistance in Pseudomonas fluorescens and Pseudomonas aeruginosa biofilms. The RNA-seq data show that efflux pumps and phosphonate degradation, lipid biosynthesis, and polyamine biosynthesis metabolic pathways were induced upon glutaraldehyde exposure. Furthermore, chemical inhibition of efflux pumps potentiates glutaraldehyde activity, suggesting that efflux activity contributes to glutaraldehyde resistance. Additionally, induction of known modulators of biofilm formation, including phosphonate degradation, lipid biosynthesis, and polyamine biosynthesis, may contribute to biofilm resistance and resilience. Fundamental understanding of the genetic mechanism of biocide resistance is critical for the optimization of biocide use and development of novel disinfection strategies. Our results reveal genetic components involved in glutaraldehyde resistance and a potential strategy for improved control of biofilms. PMID:25824217

  7. Pseudomonas cepacia adherence to respiratory epithelial cells is enhanced by Pseudomonas aeruginosa

    SciTech Connect

    Saiman, L.; Cacalano, G.; Prince, A. )

    1990-08-01

    Pseudomonas aeruginosa and Pseudomonas cepacia are both opportunistic pathogens of patients with cystic fibrosis. The binding characteristics of these two species were compared to determine if they use similar mechanisms to adhere to respiratory epithelial cells. P. cepacia 249 was shown to be piliated, but there was no detectable homology between P. aeruginosa pilin gene probes and P. cepacia genomic DNA. P. cepacia and P. aeruginosa did not appear to compete for epithelial receptors. In the presence of purified P. aeruginosa pili, the adherence of 35S-labeled strain 249 to respiratory epithelial monolayers was unaffected, while that of P. aeruginosa PAO1 was decreased by 55%. The binding of P. cepacia 249 and 715j was increased by 2.4-fold and 1.5-fold, respectively, in the presence of an equal inoculum of PAO1. Interbacterial agglutination contributed to the increased adherence of P. cepacia, as the binding of 249 was increased twofold in the presence of irradiated PAO1. PAO1 exoproducts had a marked effect in enhancing the ability of the P. cepacia strains to adhere to the epithelial monolayers. A PAO1 supernatant increased the binding of 249 by eightfold and that of 715j by fourfold. Thus, there appears to be a synergistic relationship between P. aeruginosa and P. cepacia in which PAO1 exoproducts modify the epithelial cell surface, exposing receptors and facilitating increased P. cepacia attachment.

  8. Treatment and prevention of relapses of CAPD Pseudomonas peritonitis.

    PubMed

    Pasadakis, P; Thodis, E; Eftimimiadou, A; Panagoutsos, S; Papazoglou, D; Kaliengidou, M; Kartali, S; Vargemezis, V

    1993-01-01

    Pseudomonas peritonitis in continuous ambulatory peritoneal dialysis (CAPD) can be difficult to eradicate, because it is frequently resistant to common antibiotics, inducing the loss of the peritoneal cavity in some cases. A total of 14 episodes of Pseudomonas peritonitis in 12 patients (6 male, 6 female) were treated with intraperitoneal (IP) administration of a combination of ceftazidime and tobramycin. All patients were hospitalized. The loading doses were 1000 mg/2 L of ceftazidime and 1.7 mg/kg of tobramycin, and the maintenance IP doses were 250 mg/2 L of ceftazidime and 16 mg/2 L of tobramycin. The therapy duration was 14 days. In 7 episodes (group A) no other antibiotic regimen was provided, while in the remaining 7 episodes (group B) therapy was continued with 500 mg b.i.d. of oral ciprofloxacin for the next 14 days. Pseudomonas species isolated in group A were P. alcaligenis (1), P. putida (1), P. maltophilia (1), R. cepacia (1), and unidentified (3). In group B the following Pseudomonas species were isolated: P. aeruginosa (4), P. diminuta (1), P. stutszeri (1), and unidentified (1). Recurrence of peritonitis was seen in 4 episodes of group A with 2 catheter removals, while all episodes were cured in group B. These results suggest that IP ceftazidime and tobramycin with the additional use of oral ciprofloxacin is successful in the treatment and prevention of relapses of Pseudomonas peritonitis. PMID:8105925

  9. 21 CFR 520.90d - Ampicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... infections (tracheobronchitis and tonsillitis) due to Escherichia coli, Pseudomonas spp., Proteus spp., Staphylococcus spp., and Streptococcus spp., urinary tract infections (cystitis) due to E. coli, Staphylococcus spp., Streptococcus spp., and Proteus spp.; bacterial gastroenteritis due to E. coli;...

  10. 21 CFR 520.90d - Ampicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... infections (tracheobronchitis and tonsillitis) due to Escherichia coli, Pseudomonas spp., Proteus spp., Staphylococcus spp., and Streptococcus spp., urinary tract infections (cystitis) due to E. coli, Staphylococcus spp., Streptococcus spp., and Proteus spp.; bacterial gastroenteritis due to E. coli;...

  11. 21 CFR 520.90d - Ampicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... infections (tracheobronchitis and tonsillitis) due to Escherichia coli, Pseudomonas spp., Proteus spp., Staphylococcus spp., and Streptococcus spp., urinary tract infections (cystitis) due to E. coli, Staphylococcus spp., Streptococcus spp., and Proteus spp.; bacterial gastroenteritis due to E. coli;...

  12. 21 CFR 520.90d - Ampicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... infections (tracheobronchitis and tonsillitis) due to Escherichia coli, Pseudomonas spp., Proteus spp., Staphylococcus spp., and Streptococcus spp., urinary tract infections (cystitis) due to E. coli, Staphylococcus spp., Streptococcus spp., and Proteus spp.; bacterial gastroenteritis due to E. coli;...

  13. 21 CFR 520.90d - Ampicillin trihydrate for oral suspension.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... infections (tracheobronchitis and tonsillitis) due to Escherichia coli, Pseudomonas spp., Proteus spp., Staphylococcus spp., and Streptococcus spp., urinary tract infections (cystitis) due to E. coli, Staphylococcus spp., Streptococcus spp., and Proteus spp.; bacterial gastroenteritis due to E. coli;...

  14. A Systems-Level Approach for Investigating Pseudomonas aeruginosa Biofilm Formation

    E-print Network

    Wood, Thomas K.

    A Systems-Level Approach for Investigating Pseudomonas aeruginosa Biofilm Formation Zhaobin Xu. The proposed approach was applied to identify target genes to treat Pseudomonas aeruginosa infections) A Systems-Level Approach for Investigating Pseudomonas aeruginosa Biofilm Formation. PLoS ONE 8(2): e57050

  15. Author's personal copy Phenazines affect biofilm formation by Pseudomonas aeruginosa in similar

    E-print Network

    Dietrich, Lars

    Author's personal copy Phenazines affect biofilm formation by Pseudomonas aeruginosa in similar. In this study, we compared the phenotypes of Pseudomonas aeruginosa strain PA14 and a mutant unable: Phenazine; Biofilm; Swarming 1. Introduction Pseudomonas aeruginosa is an important opportunistic human

  16. Airway epithelial tight junctions and binding and cytotoxicity of Pseudomonas aeruginosa

    E-print Network

    Machen, Terry E.

    Airway epithelial tight junctions and binding and cytotoxicity of Pseudomonas aeruginosa ALFRED LEE epithelial tight junctions and bind- ing and cytotoxicity of Pseudomonas aeruginosa. Am. J. Physiol. 277 and cytoxicity of Pseudomonas aeruginosa to apical or basolateral membranes of lung airway epithelial cells

  17. THE PSEUDOMONAS AERUGINOSA D-ARABINOFURANOSE BIOSYNTHETIC PATHWAY AND ITS ROLE IN TYPE IV PILUS ASSEMBLY

    E-print Network

    Burrows, Lori

    ! "! THE PSEUDOMONAS AERUGINOSA D-ARABINOFURANOSE BIOSYNTHETIC PATHWAY AND ITS ROLE IN TYPE IV@mcmaster.ca), 4H18 Health Sciences Centre, 1200 Main St. West, Hamilton, ON Canada L8N3Z5 Pseudomonas aeruginosa-Araf, a key therapeutic target in corynebacterineae. The gram-negative pathogen Pseudomonas aeruginosa can

  18. 40 CFR 180.1145 - Pseudomonas syringae; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pseudomonas syringae; exemption from... FOOD Exemptions From Tolerances § 180.1145 Pseudomonas syringae; exemption from the requirement of a tolerance. Pseudomonas syringae is exempted from the requirement of a tolerance on all raw...

  19. Genetically enhanced cellulase production in Pseudomonas cellulosa using recombinant DNA technology

    DOEpatents

    Dees, H. Craig (Knoxville, TN)

    1999-01-01

    An enhanced strain of Pseudomonas celllulosa was obtained by introducing a recombinant genetic construct comprising a heterologous cellulase gene operably connected to a promoter into ATCC 55702, mutagenizing the transformants by treatment with MNNG, and selecting a high cellulase producing transformant. The transformant, designated Pseudomonas cellulosa ATCC XXXX, exhibits enhanced levels of cellulase production relative to the untransformed Pseudomonas cellulosa strain #142 ATCC 55702.

  20. Generation of Reactive Oxygen Species Mediated by 1Hydroxyphenazine, a Virulence Factor of Pseudomonas aeruginosa

    E-print Network

    Gates, Kent. S.

    Pseudomonas aeruginosa is an opportunistic pathogen that infects the lungs of immunocompromised patients of Pseudomonas aeruginosa Sarmistha Sinha, Xiulong Shen, Fabio Gallazzi,§ Qian Li,, Jaroslaw W. Zmijewski, Jack R produced by Pseudomonas aeruginosa. In this study, supercoiled plasmid DNA was employed as an analytical

  1. Evolutionary and functional diversity of the Pseudomonas type IVa pilin islandemi_2327 250..264

    E-print Network

    Burrows, Lori

    Master University, Hamilton, ON, Canada. Summary In Pseudomonas aeruginosa, most proteins involved in type IVa pilus and disas- sembly are still unclear. The T4P system of Pseudomonas aeruginosa, an opportunistic GramEvolutionary and functional diversity of the Pseudomonas type IVa pilin islandemi_2327 250

  2. Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential

    E-print Network

    Dangl, Jeff

    Comparative Genomics of Multiple Strains of Pseudomonas cannabina pv. alisalensis, a Potential. Here we present a comparative analysis of draft genome sequences for four strains of Pseudomonas, Baltrus DA, Bull CT, Wechter WP, et al. (2013) Comparative Genomics of Multiple Strains of Pseudomonas

  3. The Single-Nucleotide Resolution Transcriptome of Pseudomonas aeruginosa Grown in Body Temperature

    E-print Network

    Sorek, Rotem

    The Single-Nucleotide Resolution Transcriptome of Pseudomonas aeruginosa Grown in Body Temperature Pseudomonas aeruginosa has an ability to thrive in a variety of hosts and cause a range of acute and chronic caused by the opportunistic pathogen Pseudomonas aeruginosa are determined by several factors related

  4. vol. 160, no. 5 the american naturalist november 2002 Experimental Adaptive Radiation in Pseudomonas

    E-print Network

    Fussman, Gregor

    of the bacterium Pseudomonas fluorescens derived from a single ancestral clone in 95 environments, where growth in Pseudomonas R. Craig MacLean1,* and Graham Bell1,2 1. Department of Biology, McGill University, 1205 Avenue, Pseudomonas. Adaptive radiation is the process by which ecological and genetic diversity evolves in a single

  5. An ATP and Oxalate Generating Variant Tricarboxylic Acid Cycle Counters Aluminum Toxicity in Pseudomonas

    E-print Network

    Appanna, Vasu

    in Pseudomonas fluorescens Ranji Singh, Joseph Lemire, Ryan J. Mailloux, Daniel Che´nier, Robert Hamel, Vasu D of Pseudomonas fluorescens. The upregulation of isocitrate lyase (ICL) and acylating glyoxylate dehydrogenase Tricarboxylic Acid Cycle Counters Aluminum Toxicity in Pseudomonas fluorescens. PLoS ONE 4(10): e7344. doi:10

  6. Z .Journal of Contaminant Hydrology 36 1999 7389 Transport of Pseudomonas fluorescens strain P17

    E-print Network

    Z .Journal of Contaminant Hydrology 36 1999 73­89 Transport of Pseudomonas fluorescens strain P17 Abstract Porous media column experiments were used to investigate Pseudomonas fluorescens strain P17 reserved. Keywords: Pseudomonas fluorescens strain P17; Porous media; Quartz sand 1. Introduction Research

  7. Biotransformation of Tributyltin chloride by Pseudomonas stutzeri strain DN2

    PubMed Central

    Khanolkar, Dnyanada S.; Naik, Milind Mohan; Dubey, Santosh Kumar

    2014-01-01

    A bacterial isolate capable of utilizing tributyltin chloride (TBTCl) as sole carbon source was isolated from estuarine sediments of west coast of India and identified as Pseudomonas stutzeri based on biochemical tests and Fatty acid methyl ester (FAME) analysis. This isolate was designated as strain DN2. Although this bacterial isolate could resist up to 3 mM TBTCl level, it showed maximum growth at 2 mM TBTCl in mineral salt medium (MSM). Pseudomonas stutzeri DN2 exposed to 2 mM TBTCl revealed significant alteration in cell morphology as elongation and shrinkage in cell size along with roughness of cell surface. FTIR and NMR analysis of TBTCl degradation product extracted using chloroform and purified using column chromatography clearly revealed biotransformation of TBTCl into Dibutyltin dichloride (DBTCl2) through debutylation process. Therefore, Pseudomonas stutzeri strain DN2 may be used as a potential bacterial strain for bioremediation of TBTCl contaminated aquatic environmental sites. PMID:25763027

  8. Antiadhesive properties of glycoclusters against Pseudomonas aeruginosa lung infection.

    PubMed

    Boukerb, Amine M; Rousset, Audric; Galanos, Nicolas; Méar, Jean-Baptiste; Thépaut, Marion; Grandjean, Teddy; Gillon, Emilie; Cecioni, Samy; Abderrahmen, Claire; Faure, Karine; Redelberger, David; Kipnis, Eric; Dessein, Rodrigue; Havet, Stéphane; Darblade, Benoit; Matthews, Susan E; de Bentzmann, Sophie; Guéry, Benoit; Cournoyer, Benoit; Imberty, Anne; Vidal, Sébastien

    2014-12-26

    Pseudomonas aeruginosa lung infections are a major cause of death in cystic fibrosis and hospitalized patients. Treating these infections is becoming difficult due to the emergence of conventional antimicrobial multiresistance. While monosaccharides have proved beneficial against such bacterial lung infection, the design of several multivalent glycosylated macromolecules has been shown to be also beneficial on biofilm dispersion. In this study, calix[4]arene-based glycoclusters functionalized with galactosides or fucosides have been synthesized. The characterization of their inhibitory properties on Pseudomonas aeruginosa aggregation, biofilm formation, adhesion on epithelial cells, and destruction of alveolar tissues were performed. The antiadhesive properties of the designed glycoclusters were demonstrated through several in vitro bioassays. An in vivo mouse model of lung infection provided an almost complete protection against Pseudomonas aeruginosa with the designed glycoclusters. PMID:25419855

  9. Immunogenic properties of Pseudomonas aeruginosa mucoid exopolysaccharide.

    PubMed Central

    Garner, C V; DesJardins, D; Pier, G B

    1990-01-01

    Previous studies have shown that antibodies to Pseudomonas aeruginosa mucoid exopolysaccharide (MEP) can be divided into two types on the basis of their functional activity. One type is able to mediate opsonic killing in conjunction with leukocytes and complement, and the other type is not. We investigated, in mice, the properties of this antigen associated with elicitation of opsonic killing antibody. We found that smaller-sized material (Kav = 0.26), which has been tested as a human vaccine, elicited opsonic killing antibody in mice at low doses (1 to 10 micrograms) and at doses of greater than or equal to 40 micrograms, only nonopsonic killing antibody was produced. A similar dose effect was seen with heat-killed mucoid P. aeruginosa cells. After immunization with high doses of this MEP or the heat-killed cells, the mice were refractory to induction of opsonic killing antibody no matter what dose of MEP was used as a booster. In contrast, a larger-molecular-weight preparation (Kav = 0.05) elicited opsonic killing antibody over a wide dose range (1 to 400 micrograms). Additionally, 50 micrograms of the larger-sized preparation could overcome the suppression induced by 50 micrograms of the smaller material. Suppression elicited by 50 micrograms of the smaller material could be adoptively transferred to nonimmune mice with the T-cell fraction of spleen cells. These results indicate that both molecular size and dose are critical determinants for eliciting opsonic killing antibody to mucoid P. aeruginosa after immunizing with MEP. PMID:2140341

  10. PSEUDOMONAS AERUGINOSA INFECTIONS OF THE EYE

    PubMed Central

    Spencer, William H.

    1953-01-01

    Pseudomonas aeruginosa seldom invades the body except in persons or in organs lacking natural defenses, and usually the infection is chronic rather than acute, evoking little systemic response. When introduced into the cornea, however, as in penetration by a foreign body or in contaminated medicines, it acts with extreme virulence, in many cases causing blindness and even necessitating enucleation. Although many attempts at control of Ps. aeruginosa, even with powerful antibiotics, have been unsuccessful, polymyxin B appeared to have good effect and was tested in experimental infection of the cornea in rabbits. It was demonstrated by preliminary studies in vitro that polymyxin B was effective against nine strains of Ps. aeruginosa which on inoculation caused rapidly progressive ulcers in the corneas of rabbits. A strain of proved virulence was introduced into both eyes of each of 18 rabbits. The left eyes only were treated with subconjunctival injections at 48-hour intervals of a solution of polymyxin B, to which epinephrine was added as a vasoconstrictor to prevent rapid dispersion. The right eyes remained untreated as controls. In five of the six rabbits treated immediately after inoculation, the treated eyes remained clear, while moderate infiltration developed in the sixth. In the six rabbits not treated for 24 hours after inoculation, ulcers developed but remained localized during therapy. In those not treated for 48 hours after inoculation, ulcers developed before treatment began but did not spread as rapidly as in the controls. Hyaluronidase was added to the preparation for half the rabbits in each group but had no perceptible beneficial effect. PMID:13106731

  11. Specific Genomic Fingerprints of Phosphate Solubilizing Pseudomonas Strains Generated by Box Elements

    PubMed Central

    Javadi Nobandegani, Mohammad Bagher; Saud, Halimi Mohd; Yun, Wong Mui

    2014-01-01

    Primers corresponding to conserved bacterial repetitive of BOX elements were used to show that BOX-DNA sequences are widely distributed in phosphate solubilizing Pseudomonas strains. Phosphate solubilizing Pseudomonas was isolated from oil palm fields (tropical soil) in Malaysia. BOX elements were used to generate genomic fingerprints of a variety of Pseudomonas isolates to identify strains that were not distinguishable by other classification methods. BOX-PCR, that derived genomic fingerprints, was generated from whole purified genomic DNA by liquid culture of phosphate solubilizing Pseudomonas. BOX-PCR generated the phosphate solubilizing Pseudomonas specific fingerprints to identify the relationship between these strains. This suggests that distribution of BOX elements' sequences in phosphate solubilizing Pseudomonas strains is the mirror image of their genomic structure. Therefore, this method appears to be a rapid, simple, and reproducible method to identify and classify phosphate solubilizing Pseudomonas strains and it may be useful tool for fast identification of potential biofertilizer strains. PMID:25580434

  12. MULTIPLE REPLICONS CONSTITUTING THE GENOME OF PSEUDOMONAS CEPACIA 17616

    EPA Science Inventory

    Macrorestriction fragment analysis of DNA from Pseudomonas cepacia 17616, in conjunction with Southern hybridization experiments using junction n enzyme sites as probes, indicated that this bacterium contains three large circular replicons of 3.4, 2.5, and 0.9 170-kb cryptic plas...

  13. Engineering the soil bacterium Pseudomonas putida for arsenic methylation.

    PubMed

    Chen, Jian; Qin, Jie; Zhu, Yong-Guan; de Lorenzo, Víctor; Rosen, Barry P

    2013-07-01

    Accumulation of arsenic has potential health risks through consumption of food. Here, we inserted the arsenite [As(III)] S-adenosylmethionine methyltransferase (ArsM) gene into the chromosome of Pseudomonas putida KT2440. Recombinant bacteria methylate inorganic arsenic into less toxic organoarsenicals. This has the potential for bioremediation of environmental arsenic and reducing arsenic contamination in food. PMID:23645194

  14. Draft Genome Sequence of Pseudomonas moraviensis R28-S.

    PubMed

    Hunter, Samuel S; Yano, Hirokazu; Loftie-Eaton, Wesley; Hughes, Julie; De Gelder, Leen; Stragier, Pieter; De Vos, Paul; Settles, Matthew L; Top, Eva M

    2014-01-01

    We report the draft genome sequence of Pseudomonas moraviensis R28-S, isolated from the municipal wastewater treatment plant of Moscow, ID. The strain carries a native mercury resistance plasmid, poorly maintains introduced IncP-1 antibiotic resistance plasmids, and has been useful for studying the evolution of plasmid host range and stability. PMID:24558233

  15. Draft Genome Sequence of Pseudomonas moraviensis R28-S

    PubMed Central

    Yano, Hirokazu; Loftie-Eaton, Wesley; Hughes, Julie; De Gelder, Leen; Stragier, Pieter; De Vos, Paul; Settles, Matthew L.

    2014-01-01

    We report the draft genome sequence of Pseudomonas moraviensis R28-S, isolated from the municipal wastewater treatment plant of Moscow, ID. The strain carries a native mercury resistance plasmid, poorly maintains introduced IncP-1 antibiotic resistance plasmids, and has been useful for studying the evolution of plasmid host range and stability. PMID:24558233

  16. Phosphate Starvation Promotes Swarming Motility and Cytotoxicity of Pseudomonas aeruginosa

    PubMed Central

    Bains, Manjeet; Fernández, Lucía

    2012-01-01

    We investigated the transcriptional responses of Pseudomonas aeruginosa under phosphate-deficient (0.2 mM) conditions compared to phosphate sufficiency (1 mM). This elicited enormous transcriptional changes in genes related to phosphate acquisition, quorum sensing, chemotaxis, toxin secretion, and regulation. This dysregulation also led to increased virulence-associated phenotypes, including swarming motility and cytotoxicity. PMID:22773629

  17. Characterization of two small RNAs of Pseudomonas syringae DC3000

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Small RNAs (sRNAs) are important components of many regulatory pathways and have been shown to have key roles in regulation of factors important for virulence. To date, only a few sRNAs have been described for the bacterial plant pathogen Pseudomonas syringae. One approach to identify novel candid...

  18. The Biology and Biological Activity of Pseudomonas syringae pv. tagetis

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas syringae pv. tagetis (Pst) is a disease of plants in the family Asteraceae. A distinctive characteristic of this bacterial pathogen is the symptom of apical chlorosis in infected plants, caused by the phytotoxin tagetitoxin. Strains of Pst have been isolated from several plant species ...

  19. PHENAZINE COMPOUNDS IN FLUORESCENT PSEUDOMONAS SPP.: BIOSYNTHESIS AN REGULATION

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The phenazines include upward of 50 pigmented, heterocyclic nitrogen-containing secondary metabolites synthesized by some strains of fluorescent Pseudomonas spp. and a few other bacterial genera. The antibiotic properties of these compounds have been known for over 150 years, but advances within the...

  20. Complete Genome Sequence of Biocontrol Strain Pseudomonas fluorescens LBUM 223

    PubMed Central

    Roquigny, Roxane; Arseneault, Tanya; Gadkar, Vijay J.; Novinscak, Amy

    2015-01-01

    Pseudomonas fluorescens LBUM 223 is a plant growth-promoting rhizobacterium (PGPR) with biocontrol activity against various plant pathogens. It produces the antimicrobial metabolite phenazine-1-carboxylic acid, which is involved in the biocontrol of Streptomyces scabies, the causal agent of common scab of potato. Here, we report the complete genome sequence of P. fluorescens LBUM 223. PMID:25953163

  1. Draft Genome Sequence of Rice Isolate Pseudomonas chlororaphis EA105

    PubMed Central

    McCully, Lucy M.; Bitzer, Adam S.; Spence, Carla A.; Bais, Harsh P.

    2014-01-01

    Pseudomonas chlororaphis EA105, a strain isolated from rice rhizosphere, has shown antagonistic activities against a rice fungal pathogen, and could be important in defense against rice blast. We report the draft genome sequence of EA105, which is an estimated size of 6.6 Mb. PMID:25540352

  2. Detection of Pseudomonas savastanoi pv. glycinea in soybean seeds

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This chapter is one of 52 that will compose the second edition of the Laboratory Manual for the Detection of Plant Pathogenic Bacteria from Seeds and other Planting Material, to be published by the American Phytopathological Society. The chapter presents a description of Pseudomonas savastanoi pv. ...

  3. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... 21 Food and Drugs 8 2012-04-01 2012-04-01 false Pseudomonas spp. serological reagents. 866.3415 Section 866.3415 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415...

  4. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Pseudomonas spp. serological reagents. 866.3415 Section 866.3415 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415...

  5. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... 21 Food and Drugs 8 2011-04-01 2011-04-01 false Pseudomonas spp. serological reagents. 866.3415 Section 866.3415 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415...

  6. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... 21 Food and Drugs 8 2013-04-01 2013-04-01 false Pseudomonas spp. serological reagents. 866.3415 Section 866.3415 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415...

  7. 21 CFR 866.3415 - Pseudomonas spp. serological reagents.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 8 2014-04-01 2014-04-01 false Pseudomonas spp. serological reagents. 866.3415 Section 866.3415 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IMMUNOLOGY AND MICROBIOLOGY DEVICES Serological Reagents § 866.3415...

  8. Pseudomonas seleniipraecipitans proteins potentially involved in selenite reduction

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas seleniipraecipitans grows in the presence of high levels of selenite and selenate and reduces both oxyanions to elemental selenium, a property that may make P. seleniipraecipitans useful as an inoculant for biobarriers designed to remove selenite or selenate from ground or surface-waters...

  9. Structure and function of Pseudomonas aeruginosa protein PA1324 (21170)

    E-print Network

    Powers, Robert

    Structure and function of Pseudomonas aeruginosa protein PA1324 (21­170) Kelly A. Mercier,1 John R the solution NMR structure of PA1324, a protein of unknown function identified in these studies, and provide screening studies. PA1324 is postulated to be involved in the binding and transport of sugars

  10. Genomics of Secondary Metabolite Production by Pseudomonas fluorescens Pf-5

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The rhizosphere bacterium Pseudomonas fluorescens Pf-5 is known to produce six secondary metabolites, and the genomic sequence of Pf-5 revealed three additional gene clusters, which encode for the biosynthesis of unknown natural products but contain conserved sequences of genes encoding for non-ribo...

  11. Complete Genome Sequence of Biocontrol Strain Pseudomonas fluorescens LBUM223.

    PubMed

    Roquigny, Roxane; Arseneault, Tanya; Gadkar, Vijay J; Novinscak, Amy; Joly, David L; Filion, Martin

    2015-01-01

    Pseudomonas fluorescens LBUM223 is a plant growth-promoting rhizobacterium (PGPR) with biocontrol activity against various plant pathogens. It produces the antimicrobial metabolite phenazine-1-carboxylic acid, which is involved in the biocontrol of Streptomyces scabies, the causal agent of common scab of potato. Here, we report the complete genome sequence of P. fluorescens LBUM223. PMID:25953163

  12. Pseudomonas sabulinigri sp. nov., isolated from black beach sand

    E-print Network

    Bae, Jin-Woo

    Pseudomonas sabulinigri sp. nov., isolated from black beach sand Kyoung-Ho Kim,1 Seong Woon Roh,1 , was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Cells grew at 4­37 6C, at pH 5 beach sand, a bacterium was isolated and subjected to taxonomic characterization. On the basis

  13. Functional bacterial amyloid increases Pseudomonas biofilm hydrophobicity and stiffness

    PubMed Central

    Zeng, Guanghong; Vad, Brian S.; Dueholm, Morten S.; Christiansen, Gunna; Nilsson, Martin; Tolker-Nielsen, Tim; Nielsen, Per H.; Meyer, Rikke L.; Otzen, Daniel E.

    2015-01-01

    The success of Pseudomonas species as opportunistic pathogens derives in great part from their ability to form stable biofilms that offer protection against chemical and mechanical attack. The extracellular matrix of biofilms contains numerous biomolecules, and it has recently been discovered that in Pseudomonas one of the components includes ?-sheet rich amyloid fibrils (functional amyloid) produced by the fap operon. However, the role of the functional amyloid within the biofilm has not yet been investigated in detail. Here we investigate how the fap-based amyloid produced by Pseudomonas affects biofilm hydrophobicity and mechanical properties. Using atomic force microscopy imaging and force spectroscopy, we show that the amyloid renders individual cells more resistant to drying and alters their interactions with hydrophobic probes. Importantly, amyloid makes Pseudomonas more hydrophobic and increases biofilm stiffness 20-fold. Deletion of any one of the individual members of in the fap operon (except the putative chaperone FapA) abolishes this ability to increase biofilm stiffness and correlates with the loss of amyloid. We conclude that amyloid makes major contributions to biofilm mechanical robustness. PMID:26500638

  14. Genome Sequence of Mucoid Pseudomonas aeruginosa Strain FRD1.

    PubMed

    Wang, Di; Hildebrand, Falk; Ye, Lumeng; Wei, Qing; Ma, Luyan Z

    2015-01-01

    Pseudomonas aeruginosa is an important opportunistic pathogen. Strain FRD1 is a mucoid isolate from the sputum of a cystic fibrosis patient. It has been widely studied and has many different phenotypes compared to nonmucoid strains. Here, we present the draft genome sequence of P. aeruginosa strain FRD1 to gain insight into mucoid isolates. PMID:25908149

  15. Shotgun Sequencing of the Pseudomonas syringae DC3000 Transcriptome

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas syringae pv. tomato strain DC3000 is a bacterial plant pathogen capable of causing disease in tomatoes and Arabidopsis. The genome of this bacterium has been sequenced, however as with other genomes, accurate annotation and determination of coding vs. non-coding regions has proven to be...

  16. Integron-Mediated Rifampin Resistance in Pseudomonas aeruginosa

    PubMed Central

    Tribuddharat, Chanwit; Fennewald, Michael

    1999-01-01

    A new rifampin resistance gene, arr-2, has been found in Pseudomonas aeruginosa. The ARR-2 protein shows 54% amino acid identity to the rifampin ADP-ribosylating transferase encoded by the arr gene from Mycobacterium smegmatis. This arr-2 gene is located on a gene cassette within a class I integron. PMID:10103210

  17. BIOGEOGRAPHY OF 2,4-DIACETYLPHLOROGLUCINOL-PRODUCING PSEUDOMONAS FLUORESCENS.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Strains of Pseudomonas fluorescens producing the antibiotic 2,4-diacetylphloroglucinol (phlD+) are biocontrol agents of soilborne pathogens and play a key role in the disease suppressiveness of some soils. Considerable variation among isolates has been observed by using genomic fingerprinting techn...

  18. EXPRESSION OF DEGRADATIVE GENES OF 'PSEUDOMONAS PUTIDA' IN 'CAULOBACTER CRESCENTUS'

    EPA Science Inventory

    The recombinant plasmid RP4-TOL was transferred into Caulobacter crescentus at a high frequency, and the plasmid was maintained for at least 50 generations. C. crescentus cells which contained RP4-TOL grew on all the aromatic compounds that the plasmid normally allowed Pseudomona...

  19. Chemotaxis to furan compounds by furan-degrading Pseudomonas strains

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two Pseudomonas strains known to utilize furan derivatives were shown to be attracted to furfural, 5-hydroxymethylfurfural, furfuryl alcohol, and 2-furoic acid in the absence of furan metabolism. In addition, a LysR-family regulatory protein known to regulate furan metabolic genes was found to be i...

  20. Metabolism of volatile chlorinated aliphatic hydrocarbons by Pseudomonas fluorescens

    SciTech Connect

    Vandenbergh, P.A.; Kunka, B.S. )

    1988-10-01

    A Pseudomonas fluorescens strain designated PFL12 was isolated at a landfill site from soil and water that were contaminated with various chloroaliphatic hydrocarbons. The isolate was able to metabolize 1,2-dichloroethane, 1,1,2-trichloroethane, 1,2-dichloropropane, 2,2-dichloropropane, and trichloroethylene.

  1. Discontinuities in the evolution of Pseudomonas putida cat genes.

    PubMed Central

    Houghton, J E; Brown, T M; Appel, A J; Hughes, E J; Ornston, L N

    1995-01-01

    The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC begins 21 nucleotides downstream from catB, and catA begins 41 nucleotides downstream from catC. This contrasts with the gene arrangement in other bacteria, in which catA lies several kilobases upstream from catB. Properties of Tn5 mutants confirmed earlier suggestions that catR is a transcriptional activator and indicated that catA is activated by CatR independently of its activation of catBC. CatR binds to both a DNA fragment containing the catR-catB intergenic region and another DNA fragment containing catC. Pseudomonas strain RB1 resembles P. putida in some respects. Divergence of the two Pseudomonas chromosomes was revealed as nucleotide substitution of about 10% after alignment of known portions of catR,BC,A. Divergent transcriptional controls are suggested by a cluster of nucleotide sequence modifications in Pseudomonas strain RB1 which disrupt a stem-loop structure directly upstream of catB in the P. putida chromosome. Abrupt divergence of the catR,BC,A nucleotide sequences was achieved during evolution by insertion of an 85-bp palindromic genetic element uniquely positioned downstream from P. putida catR and counterpoised by insertion of a similar palindromic sequence in the Pseudomonas strain RB1 catB-catC intergenic region. Properties of the palindromic genetic element suggest that it may serve functions analogous to those of repetitive extragenic palindromic sequences and enteric repetitive intergenic consensus sequences in enteric bacteria. PMID:7814330

  2. High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species

    PubMed Central

    Bultreys, Alain; Gheysen, Isabelle; Wathelet, Bernard; Maraite, Henri; de Hoffmann, Edmond

    2003-01-01

    The relationship of pyoverdins produced by 41 pathovars of Pseudomonas syringae and by phytopathogenic Pseudomonas species was investigated. A high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typical Fe(III)-chelated pyoverdins. Nonfluorescent strains were found in Pseudomonas amygdali, Pseudomonas meliae, Pseudomonas fuscovaginae, and P. syringae. Pseudomonas agarici and Pseudomonas marginalis produced typical pyoverdins. Among the arginine dihydrolase-negative fluorescent Pseudomonas species, spectral, amino acid, and mass spectrometry analyses underscored for the first time the clear similarities among the pyoverdins produced by related species. Within this group, the oxidase-negative species Pseudomonas viridiflava and Pseudomonas ficuserectae and the pathovars of P. syringae produced the same atypical pyoverdin, whereas the oxidase-positive species Pseudomonas cichorii produced a similar atypical pyoverdin that contained a glycine instead of a serine. The more distantly related species Pseudomonas asplenii and Pseudomonas fuscovaginae both produced a less similar atypical pyoverdin. The spectral characteristics of Fe(III)-chelated atypical pyoverdins at pH 7.0 were related to the presence of two ?-hydroxyaspartic acids as iron ligands, whereas in typical pyoverdins one of the ligands is always ornithine based. The peptide chain influenced the chelation of iron more in atypical pyoverdins. Our results demonstrated that there is relative pyoverdin conservation in the amino acids involved in iron chelation and that there is faster evolution of the other amino acids, highlighting the usefulness of pyoverdins in systematics and in identification. PMID:12571041

  3. Transfer and occurrence of large mercury resistance plasmids in river epilithon. [Pseudomonas fluorescens; Pseudomonas putida

    SciTech Connect

    Bale, M.J.; Fry, J.C.; Day, M.J.

    1988-04-01

    In situ mating experiments were done in the River Taff, South Wales, United Kingdom, by using a natural mercury resistance plasmid (pQM1) isolated from a mixture of epilithic bacteria in vitro. The river temperature from March to November was found to influence transfer frequencies strongly (6.8 x 10/sup -9/ to 1.5 x 10/sup -2/ per recipient). A linear relationship existed between log/sub 10/ transfer frequency and river temperature (6 to 21/sup 0/C), a 2.6/sup 0/C change in temperature giving a 10-fold change in transfer frequency. In vitro experiments showed that pQM1 transferred most efficiently between fluorescent pseudomonads and that one epilithic isolate (Pseudomonas fluorescens) was an efficient donor in situ. Experiments with a P. putida recipient showed that intact epilithic bacterial communities could transfer mercury resistance plasmids in situ at frequencies of up to 3.75 x 10/sup -6/ per recipient. Nineteen of the large (>250-kilobase) plasmids isolated by transfer into P. putida were studied in detail and grouped into seven types by restriction digests. Mercury resistance and UV resistance were found to be common linked phenotypes in 19 of the 23 plasmids tested.

  4. 21 CFR 522.90a - Ampicillin trihydrate sterile suspension.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...Indications for use. Treatment of respiratory tract infections due to E. coli, Pseudomonas spp., Proteus spp., Staphylococcus ...and Staphylococcus spp.; generalized infections (septicemia) associated with abscesses,...

  5. Structure of a putative acetyltransferase (PA1377) from Pseudomonas aeruginosa

    SciTech Connect

    Davies, Anna M.; Tata, Renée; Chauviac, François-Xavier; Sutton, Brian J.; Brown, Paul R.

    2008-05-01

    The crystal structure of an acetyltransferase encoded by the gene PA1377 from Pseudomonas aeruginosa has been determined at 2.25 Ĺ resolution. Comparison with a related acetyltransferase revealed a structural difference in the active site that was taken to reflect a difference in substrate binding and/or specificity between the two enzymes. Gene PA1377 from Pseudomonas aeruginosa encodes a 177-amino-acid conserved hypothetical protein of unknown function. The structure of this protein (termed pitax) has been solved in space group I222 to 2.25 Ĺ resolution. Pitax belongs to the GCN5-related N-acetyltransferase family and contains all four sequence motifs conserved among family members. The ?-strand structure in one of these motifs (motif A) is disrupted, which is believed to affect binding of the substrate that accepts the acetyl group from acetyl-CoA.

  6. Serotypes of Pseudomonas aeruginosa isolated from laboratory rats and mice.

    PubMed

    Batema, R P; Wagner, J E; Trease, F W; Lentsch, R H

    1983-12-01

    Pseudomonas aeruginosa isolates cultured from the feces of laboratory rats and mice were serotyped. The fecal samples originated from primary genetic centers, secondary breeding facilities, and research testing facilities operated under contracts from the National Cancer Institute. Eighty-nine percent or 264 of 297 isolates were of serotypes 1, 4, 6, 10, or 11, and of these, 154 (51.8%) isolates were serotypes 6 or 11. In some instances, Pseudomonas aeruginosa serotypes found in animals at a primary genetic center were also found at secondary breeding facilities which had received breeding stock from the primary genetic center. The same serotypes also were found in animals at research-testing laboratories that had received animals from the secondary breeding facilities. PMID:6420617

  7. Simple DNA transformation in Pseudomonas based on the Yoshida effect.

    PubMed

    Rodríguez-Beltrán, Jerónimo; Elabed, Hamouda; Gaddour, Kamel; Blázquez, Jesús; Rodríguez-Rojas, Alexandro

    2012-05-01

    Current protocols of recombinant DNA research, including gene cloning and complementation, quantification of gene expression and tagging with reporter proteins, are usually limited by the availability of effective bacteria transformation tools different from Escherichia coli. This is particularly relevant with respect to the Pseudomonas species due to their biotechnological and sanitary importance. Here, we describe an optimized and efficient plasmid transference protocol based on the Yoshida effect, a method that relies on DNA uptake mediated by friction forces. The main advantages of this method are: (i) no competent cell preparation is needed, (ii) cells in any physiological state can be used, (iii) the procedure is performed directly on agar plates and (iv) the protocol, which is neither time-consuming nor labor-intensive, offers good efficiency. This approach promises to become the gold standard for day to day genetic manipulation in Pseudomonas. PMID:22405834

  8. Penetration and growth of Pseudomonas fluorescens in chicken eggs 

    E-print Network

    Mountney, George Joseph

    1957-01-01

    and Xfas304 as compared to Pseudomonas phage phiKMV and Caulobacter phage Cd1 ................................................................... 56 FIG 5 Bacterial colony edge morphology and twitching motility ................................... 77... of this previously unknown disease suggests that the causal agent was an introduced strain of X. fastidiosa (16). Another theory, based on the close genetic homology of coffee strains of X. fastidiosa to citrus strains (39), is that the citrus strains may have...

  9. Segregation of the ability to degrade lupanine in Pseudomonas lupanini.

    PubMed

    Droese, J

    1977-01-01

    Two types of mutants differing in the ability to degrade lupanine were obtained from the JD1 strain of Pseudomonas lupanini: lus growing slowly on lupanine, and lun unable to utilize lupanine as a source of carbon and nitrogen. The mutation rate for the spontaneous lus mutants was 0.28%, and for the mitomycin C-induced lus mutants, 3-24%. Rifampicin induced solely lun mutation. The plasmid nature of the lupanine degradation pathway is discussed. PMID:930514

  10. Production of glycolipids containing biosurfactant by Pseudomonas species.

    PubMed

    Ellaiah, P; Prabhakar, T; Sreekanth, M; Taleb, A Thaer; Raju, P Bhima; Saisha, V

    2002-09-01

    Microorganisms, that degrade hydrocarbon were isolated and screened for their biosurfactant activity. A total of 68 strains were isolated and tested for their glycolipid activity of which 4 isolates showed good glycolipid activity. Isolate K10 gave the maximum biosurfactant production in medium A (containing kerosene as a sole carbon source) as compared to medium B (containing glucose as a sole carbon source). Characterization of isolate K10 showed that it belongs to Pseudomonas species. PMID:12587744

  11. Capability of mucoid Pseudomonas aeruginosa to survive in chlorinated water.

    PubMed

    Grobe, S; Wingender, J; Flemming, H C

    2001-11-01

    Mucoid strains of Pseudomonas aeruginosa are characterized by an overproduction of the extracellular polysaccharide alginate. When suspended into chlorinated swimming-pool water or drinking water samples, mucoid bacteria revealed enhanced survival compared with isogenic nonmucoid cells. Removal of slime from mucoid bacteria abolished chlorine resistance, addition of purified alginate to washed bacteria again enhanced survival. Thus, alginate-containing slime confers protection on P. aeruginosa against chlorine and may contribute to survival of these bacteria in chlorinated water systems. PMID:11759157

  12. Genome Sequence of the Urethral Isolate Pseudomonas aeruginosa RN21

    PubMed Central

    Wibberg, Daniel; Tielen, Petra; Narten, Maike; Schobert, Max; Blom, Jochen; Schatschneider, Sarah; Meyer, Ann-Kathrin; Neubauer, Rüdiger; Albersmeier, Andreas; Albaum, Stefan; Jahn, Martina; Goesmann, Alexander; Vorhölter, Frank-Jörg; Pühler, Alfred

    2015-01-01

    Pseudomonas aeruginosa is known to cause complicated urinary tract infections (UTI). The improved 7.0-Mb draft genome sequence of P. aeruginosa RN21, isolated from a patient with an acute UTI, was determined. It carries three (pro)phage genomes, genes for two restriction/modification systems, and a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. PMID:26184943

  13. Detection of Pseudomonas aeruginosa eye infections by ultraviolet light

    SciTech Connect

    Weiss, J.N.; Kreter, J.K.; Dalton, H.P.; Dishler, J.

    1982-03-01

    Pseudomonas aeruginosa elaborates several pigment fractions that fluoresce in ultraviolet light. This fact has been used by surgeons in burn units so that treatment of subclinical P aeruginosa infections may be initiated promptly. We have determined the minimum in vitro concentration of two strains of this organism that exhibit ultraviolet light fluorescence and have applied this principle to the early detection of P aeruginosa eye infections.

  14. Genome Sequence of the Urethral Isolate Pseudomonas aeruginosa RN21.

    PubMed

    Wibberg, Daniel; Tielen, Petra; Narten, Maike; Schobert, Max; Blom, Jochen; Schatschneider, Sarah; Meyer, Ann-Kathrin; Neubauer, Rüdiger; Albersmeier, Andreas; Albaum, Stefan; Jahn, Martina; Goesmann, Alexander; Vorhölter, Frank-Jörg; Pühler, Alfred; Jahn, Dieter

    2015-01-01

    Pseudomonas aeruginosa is known to cause complicated urinary tract infections (UTI). The improved 7.0-Mb draft genome sequence of P. aeruginosa RN21, isolated from a patient with an acute UTI, was determined. It carries three (pro)phage genomes, genes for two restriction/modification systems, and a clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. PMID:26184943

  15. Effects of atmospheric conditions on ice nucleation activity of Pseudomonas

    NASA Astrophysics Data System (ADS)

    Attard, E.; Yang, H.; Delort, A.-M.; Amato, P.; Pöschl, U.; Glaux, C.; Koop, T.; Morris, C. E.

    2012-11-01

    Although ice nuclei from bacterial origin are known to be efficient at the highest temperatures known for ice catalysts, quantitative data are still needed to assess their role in cloud processes. Here we studied the effects of three typical cloud conditions (i) acidic pH (ii) NO2 and O3 exposure and (iii) UV-A exposure on the ice nucleation activity (INA) of four Pseudomonas strains. Three of the Pseudomonas syringae strains were isolated from cloud water and the phyllosphere and Pseudomonas fluorescens strain CGina-01 was isolated from Antarctic glacier ice melt. Among the three conditions tested, acidic pH caused the most significant effects on INA likely due to denaturation of the ice nucleation protein complex. Exposure to NO2 and O3 gases had no significant or only weak effects on the INA of two P. syringae strains whereas the INA of P. fluorescens CGina-01 was significantly affected. The INA of the third P. syringae strain showed variable responses to NO2 and O3 exposure. These differences in the INA of different Pseudomonas suggest that the response to atmospheric conditions could be strain-specific. After UV-A exposure, a substantial loss of viability of all four strains was observed whereas their INA decreased only slightly. This corroborates the notion that under certain conditions dead bacterial cells can maintain their INA. Overall, the negative effects of the three environmental factors on INA were more significant at the warmer temperatures. Our results suggest that in clouds where temperatures are near 0 °C, the importance of bacterial ice nucleation in precipitation processes could be reduced by some environmental factors.

  16. Effects of atmospheric conditions on ice nucleation activity of Pseudomonas

    NASA Astrophysics Data System (ADS)

    Attard, E.; Yang, H.; Delort, A.-M.; Amato, P.; Pöschl, U.; Glaux, C.; Koop, T.; Morris, C. E.

    2012-04-01

    Although ice nuclei from bacterial origin are known to be efficient at the highest temperatures known for ice catalysts, quantitative data are still needed to assess their role in cloud processes. Here we studied the effects of three typical cloud conditions (i) acidic pH (ii) NO2 and O3 exposure and (iii) UV-A exposure on the ice nucleation activity (INA) of four Pseudomonas strains. Three of the Pseudomonas syringae strains were isolated from cloud water and the phyllosphere and Pseudomonas fluorescens strain CGina-01 was isolated from Antarctic glacier ice melt. Among the three conditions tested, acidic pH caused the most significant effects on INA likely due to denaturation of the ice nucleation protein complex. Exposure to NO2 and O3 gases had no significant or only weak effects on the INA of two P. syringae strains whereas the INA of P. fluorescens CGina-01 was significantly affected. The INA of the third P. syringae strain showed variable responses to NO2 and O3 exposure. These differences in the INA of different Pseudomonas suggest that the response to atmospheric conditions could be strain-specific. After UV-A exposure, a substantial loss of viability of all four strains was observed whereas their INA decreased only slightly. This corroborates the notion that under certain conditions dead bacterial cells can maintain their INA. Overall, the negative effects of the three environmental factors on INA were more significant at the warmer temperatures. Our results suggest that in clouds where temperatures are near 0 °C, the importance of bacterial ice nucleation in precipitation processes could be reduced by some environmental factors.

  17. Plasmid Involvement in Linalool Metabolism by Pseudomonas fluorescens

    PubMed Central

    Vandenbergh, Peter A.; Cole, Ronald L.

    1986-01-01

    A bacterial strain was isolated from a wastewater lagoon and identified as Pseudomonas fluorescens. This isolate was able to utilize linalool as a sole carbon and energy source. The ability was found to be encoded on a 60-megadalton transmissible plasmid, pSRQ60. The plasmid was also mated into a commercial waste treatment strain, which expanded its ability to utilize other isoprenoid compounds. Images PMID:16347186

  18. Preparation and characterization of new biologically active polyurethane foams.

    PubMed

    Savelyev, Yuri; Veselov, Vitali; Markovskaya, Ludmila; Savelyeva, Olga; Akhranovich, Elena; Galatenko, Natalya; Robota, Ludmila; Travinskaya, Tamara

    2014-12-01

    Biologically active polyurethane foams are the fast-developed alternative to many applications of biomedical materials. Due to the polyurethane structure features and foam technology it is possible to incorporate into their structure the biologically active compounds of target purpose via structural-chemical modification of macromolecule. A series of new biologically active polyurethane foams (PUFs) was synthesized with polyethers (MM 2500-5000), polyesters MM (500-2200), 2,4(2,6) toluene diisocyanate, water as a foaming agent, catalysts, foam stabilizers and functional compounds. Different functional compounds: 1,4-di-N-oxy-2,3-bis-(oxymethyl)-quinoxaline (DOMQ), partial sodium salt of poly(acrylic acid) and 2,6-dimethyl-N,N-diethyl aminoacetatanilide hydrochloride were incorporated into the polymer structure/composition due to the chemical and/or physical bonding. Structural peculiarities of PUFs were studied by FTIR spectroscopy and X-ray scattering. Self-adhesion properties of PUFs were estimated by measuring of tensile strength at break of adhesive junction. The optical microscopy method was performed for the PUF morphology studies. Toxicological estimation of the PUFs was carried out in vitro and in vivo. The antibacterial action towards the Gram-positive and Gram-negative bacteria (Escherichia coli ATC 25922, E. coli ATC 2150, Klebsiella pneumoniae 6447, Staphylococcus aureus 180, Pseudomonas aeruginosa 8180, Proteus mirabilis F 403, P. mirabilis 6054, and Proteus vulgaris 8718) was studied by the disc method on the solid nutrient. Physic-chemical properties of the PUFs (density, tensile strength and elongation at break, water absorption and vapor permeability) showed that all studied PUFs are within the operational requirements for such materials and represent fine-cellular foams. Spectral studies confirmed the incorporation of DOMQ into the PUF's macrochain. PUFs are characterized by microheterogeneous structure. They are antibacterially active, non-toxic materials with high affinity to the tissue body, self-adhesive properties and local anesthetic effect. PMID:25491811

  19. Analysis of the sodium dodecyl sulfate-stable peptidoglycan autolysins of select gram-negative pathogens by using renaturing polyacrylamide gel electrophoresis.

    PubMed Central

    Bernadsky, G; Beveridge, T J; Clarke, A J

    1994-01-01

    For the first time, peptidoglycan autolysins from cellular fractions derived from sonicated cultures of Pseudomonas aeruginosa PAO1, Escherichia coli W7, Klebsiella pneumoniae CWK2, and Proteus mirabilis 19 were detected and partially characterized by zymogram analysis. Purified murein sacculi from P. aeruginosa PAO1 were incorporated into a sodium dodecyl sulfate (SDS)-polyacrylamide gel at a concentration of 0.05% (wt/vol) to serve as a substrate for the separated autolysins. At least 11 autolysin bands of various intensities with M(r)s ranging between 17,000 and 122,000 were detected in each of the homogenated cultures. Some of the autolysins of the four bacteria had similar M(r)s. The zymogram analysis was used to show that a number of the autolysins from E. coli were inhibited by the heavy metals Hg2+ and Cu2+, at 1 and 10 mM, respectively, high ionic strengths, and reagents known to affect the packing of lipopolysaccharides. The activity of an autolysin with an M(r) of 65,000 was also impaired by penicillin G, whereas it was enhanced by gentamicin. A preliminary screen to determine the relationship between penicillin-binding proteins (PBPs) and autolysins was carried out by using a dual assay in which radiolabelled penicillin V bands were visualized on an autolysin zymogram. Radiolabelled bands corresponding to PBPs 3, 4, 5, and 6 from E. coli and P. aeruginosa; PBPs 3, 4, and 6 from Proteus mirabilis; and PBP 6 from K. pneumoniae degraded the murein sacculi in the gels and were presumed to have autolytic activity, although the possibility of two distinct enzymes, each with one of the activities, comigrating in the SDS-polyacrylamide gels could not be excluded. Some radiolabelled bands possessed an Mr of <34,000 and coincided with similar low-Mr autolysin bands. Images PMID:7915268

  20. A Pseudomonas aeruginosa Toxin that Hijacks the Host Ubiquitin Proteolytic System

    E-print Network

    Bomberger, Jennifer M.

    Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen chronically infecting the lungs of patients with chronic obstructive pulmonary disease (COPD), pneumonia, cystic fibrosis (CF), and bronchiectasis. Cif ...

  1. Biomolecular Mechanisms of Pseudomonas aeruginosa and Escherichia coli Biofilm Formation

    PubMed Central

    Laverty, Garry; Gorman, Sean P.; Gilmore, Brendan F.

    2014-01-01

    Pseudomonas aeruginosa and Escherichia coli are the most prevalent Gram-negative biofilm forming medical device associated pathogens, particularly with respect to catheter associated urinary tract infections. In a similar manner to Gram-positive bacteria, Gram-negative biofilm formation is fundamentally determined by a series of steps outlined more fully in this review, namely adhesion, cellular aggregation, and the production of an extracellular polymeric matrix. More specifically this review will explore the biosynthesis and role of pili and flagella in Gram-negative adhesion and accumulation on surfaces in Pseudomonas aeruginosa and Escherichia coli. The process of biofilm maturation is compared and contrasted in both species, namely the production of the exopolysaccharides via the polysaccharide synthesis locus (Psl), pellicle Formation (Pel) and alginic acid synthesis in Pseudomonas aeruginosa, and UDP-4-amino-4-deoxy-l-arabinose and colonic acid synthesis in Escherichia coli. An emphasis is placed on the importance of the LuxR homologue sdiA; the luxS/autoinducer-II; an autoinducer-III/epinephrine/norepinephrine and indole mediated Quorum sensing systems in enabling Gram-negative bacteria to adapt to their environments. The majority of Gram-negative biofilms consist of polysaccharides of a simple sugar structure (either homo- or heteropolysaccharides) that provide an optimum environment for the survival and maturation of bacteria, allowing them to display increased resistance to antibiotics and predation. PMID:25438014

  2. Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates

    PubMed Central

    Ghequire, Maarten G.K.; Dillen, Yörg; Lambrichts, Ivo; Proost, Paul; Wattiez, Ruddy; De Mot, René

    2015-01-01

    Bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. Such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated DNA. A subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. Functional characterization of bactericidal tailocins of two Pseudomonas putida rhizosphere isolates revealed not only extensive similarity with the tail assembly module of the Pseudomonas aeruginosa R-type pyocins but also differences in genomic integration site, regulatory genes, and lytic release modules. Conversely, these three features are quite similar between strains of the P. putida and Pseudomonas fluorescens clades, although phylogenetic analysis of tail genes suggests them to have evolved separately. Unlike P. aeruginosa R pyocin elements, the tailocin gene clusters of other pseudomonads frequently carry cargo genes, including bacteriocins. Compared with P. aeruginosa, the tailocin tail fiber sequences that act as specificity determinants have diverged much more extensively among the other pseudomonad species, mostly isolates from soil and plant environments. Activity of the P. putida antibacterial particles requires a functional lipopolysaccharide layer on target cells, but contrary to R pyocins from P. aeruginosa, strain susceptibilities surpass species boundaries. PMID:26412856

  3. Different Ancestries of R Tailocins in Rhizospheric Pseudomonas Isolates.

    PubMed

    Ghequire, Maarten G K; Dillen, Yörg; Lambrichts, Ivo; Proost, Paul; Wattiez, Ruddy; De Mot, René

    2015-01-01

    Bacterial genomes accommodate a variety of mobile genetic elements, including bacteriophage-related clusters that encode phage tail-like protein complexes playing a role in interactions with eukaryotic or prokaryotic cells. Such tailocins are unable to replicate inside target cells due to the lack of a phage head with associated DNA. A subset of tailocins mediate antagonistic activities with bacteriocin-like specificity. Functional characterization of bactericidal tailocins of two Pseudomonas putida rhizosphere isolates revealed not only extensive similarity with the tail assembly module of the Pseudomonas aeruginosa R-type pyocins but also differences in genomic integration site, regulatory genes, and lytic release modules. Conversely, these three features are quite similar between strains of the P. putida and Pseudomonas fluorescens clades, although phylogenetic analysis of tail genes suggests them to have evolved separately. Unlike P. aeruginosa R pyocin elements, the tailocin gene clusters of other pseudomonads frequently carry cargo genes, including bacteriocins. Compared with P. aeruginosa, the tailocin tail fiber sequences that act as specificity determinants have diverged much more extensively among the other pseudomonad species, mostly isolates from soil and plant environments. Activity of the P. putida antibacterial particles requires a functional lipopolysaccharide layer on target cells, but contrary to R pyocins from P. aeruginosa, strain susceptibilities surpass species boundaries. PMID:26412856

  4. Aerobic Microbial Community of Insectary Population of Phlebotomus papatasi

    PubMed Central

    Maleki-Ravasan, Naseh; Oshaghi, Mohammad Ali; Hajikhani, Sara; Saeidi, Zahra; Akhavan, Amir Ahmad; Gerami-Shoar, Mohsen; Shirazi, Mohammad Hasan; Yakhchali, Bagher; Rassi, Yavar; Afshar, Davoud

    2014-01-01

    Background: Microbes particularly bacteria presenting in the gut of haematophagous insects may have an important role in the epidemiology of human infectious disease. Methods: The microbial flora of gut and surrounding environmental of a laboratory strain of Phlebotomus papatasi, the main vector of Zoonotic Cutaneous Leishmaniasis (ZCL) in the old world, was investigated. Biochemical reactions and 16s rDNA sequencing of the isolated bacteria against 24 sugars and amino acids were used for bacteria species identification. Common mycological media used for fungi identification as well. Results: Most isolates belonged to the Enterobacteriaceae, a large, heterogeneous group of gram-negative rods whose natural habitat is the intestinal tract of humans and animals. Enterobacteriaceae groups included Edwardsiella, Enterobacter, Escherichia, Klebsiella, Kluyvera, Leminorella, Pantoea, Proteus, Providencia, Rahnella, Serratia, Shigella, Tatumella, and Yersinia and non Enterobacteriaceae groups included Bacillus, Staphylococcus and Pseudomonas. The most prevalent isolates were Proteus mirabilis and P. vulgaris. These saprophytic and swarming motile bacteria were isolated from all immature, pupae, and mature fed or unfed male or female sand flies as well as from larval and adult food sources. Five fungi species were also isolated from sand flies, their food sources and colonization materials where Candida sp. was common in all mentioned sources. Conclusion: Midgut microbiota are increasingly seen as an important factor for modulating vector competence in insect vectors so their possible effects of the mirobiota on the biology of P. papatasi and their roles in the sandfly-Leishmania interaction are discussed. PMID:25629067

  5. Surveillance of multidrug resistant uropathogenic bacteria in hospitalized patients in Indian

    PubMed Central

    Mishra, Monali Priyadarsini; Debata, Nagen Kumar; Padhy, Rabindra Nath

    2013-01-01

    Objective To record surveillance, antibiotic resistance of uropathogens of hospitalized patients over a period of 18 months. Methods Urine samples from wards and cabins were used for isolating urinary tract infection (UTI)-causing bacteria that were cultured on suitable selective media and identified by biochemical tests; and their antibiograms were ascertained by Kirby-Bauer's disc diffusion method, in each 6-month interval of the study period, using 18 antibiotics of five different classes. Results From wards and cabins, 1?245 samples were collected, from which 996 strains of bacteria belonging to 11 species were isolated, during April 2011 to September 2012. Two Gram-positive, Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), and nine Gram-negative bacteria, Acinetobacter baumannii, Citrobacter sp., Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus mirabilis, Proteus vulgaris and Pseudomonas aeruginosa were isolated. Both S. aureus and E. faecalis were vancomycin resistant, and resistant-strains of all pathogens increased in each 6-month period of study. Particularly, all Gram-negatives were resistant to nitrofurantoin and co-trimoxazole, the most preferred antibiotics of empiric therapy for UTI. Conclusions Antibiograms of 11 UTI-causing bacteria recorded in this study indicated moderately higher numbers of strains resistant to each antibiotic studied, generating the fear of precipitating fervent episodes in public health particularly with bacteria, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae and S. aureus. Moreover, vancomycin resistance in strains of S. aureus and E. faecalis is a matter of concern. PMID:23620859

  6. Comparative genomic analysis of multiple strains of two unusual plant pathogens: Pseudomonas corrugata and Pseudomonas mediterranea

    PubMed Central

    Trantas, Emmanouil A.; Licciardello, Grazia; Almeida, Nalvo F.; Witek, Kamil; Strano, Cinzia P.; Duxbury, Zane; Ververidis, Filippos; Goumas, Dimitrios E.; Jones, Jonathan D. G.; Guttman, David S.; Catara, Vittoria; Sarris, Panagiotis F.

    2015-01-01

    The non-fluorescent pseudomonads, Pseudomonas corrugata (Pcor) and P. mediterranea (Pmed), are closely related species that cause pith necrosis, a disease of tomato that causes severe crop losses. However, they also show strong antagonistic effects against economically important pathogens, demonstrating their potential for utilization as biological control agents. In addition, their metabolic versatility makes them attractive for the production of commercial biomolecules and bioremediation. An extensive comparative genomics study is required to dissect the mechanisms that Pcor and Pmed employ to cause disease, prevent disease caused by other pathogens, and to mine their genomes for genes that encode proteins involved in commercially important chemical pathways. Here, we present the draft genomes of nine Pcor and Pmed strains from different geographical locations. This analysis covered significant genetic heterogeneity and allowed in-depth genomic comparison. All examined strains were able to trigger symptoms in tomato plants but not all induced a hypersensitive-like response in Nicotiana benthamiana. Genome-mining revealed the absence of type III secretion system and known type III effector-encoding genes from all examined Pcor and Pmed strains. The lack of a type III secretion system appears to be unique among the plant pathogenic pseudomonads. Several gene clusters coding for type VI secretion system were detected in all genomes. Genome-mining also revealed the presence of gene clusters for biosynthesis of siderophores, polyketides, non-ribosomal peptides, and hydrogen cyanide. A highly conserved quorum sensing system was detected in all strains, although species specific differences were observed. Our study provides the basis for in-depth investigations regarding the molecular mechanisms underlying virulence strategies in the battle between plants and microbes. PMID:26300874

  7. Pseudomonas salina sp. nov., isolated from a salt lake.

    PubMed

    Zhong, Zhi-Ping; Liu, Ying; Hou, Ting-Ting; Liu, Hong-Can; Zhou, Yu-Guang; Wang, Fang; Liu, Zhi-Pei

    2015-09-01

    A Gram-staining-negative, facultatively aerobic bacterium, strain XCD-X85T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9??%, w/v) in Qaidam basin, Qinghai province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X85T were non-endospore-forming rods, 0.4-0.6??m wide and 1.0-1.6??m long, and motile by means of a single polar flagellum. Strain XCD-X85T was catalase- and oxidase-positive. Growth was observed in the presence of 0-12.0??% (w/v) NaCl (optimum, 1.0-2.0??%) and at 4-35?°C (optimum, 25-30?°C) and pH?6.5-10.5 (optimum, pH?8.0-8.5). Strain XCD-X85T contained (>10??%) summed feature 8 (C18?:?1?7c and/or C18?:?1?6c), C12?:?0, C16?:?0 and summed feature 3 (C16?:?1?7c and/or C16?:?1?6c) as the predominant fatty acids. The major respiratory quinone was ubiquinone 9 (Q-9). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content was 57.4?mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X85T was associated with the genus Pseudomonas, and showed highest 16S rRNA gene sequence similarities to Pseudomonas pelagia CL-AP6T (99.0??%) and Pseudomonas bauzanensis BZ93T (96.8??%). DNA-DNA relatedness of strain XCD-X85T to P. pelagia JCM 15562T was 19?±?1??%. On the basis of the data presented above, it is concluded that strain XCD-X85T represents a novel species of the genus Pseudomonas, for which the name Pseudomonas salina sp. nov. is proposed. The type strain is XCD-X85T (?=?CGMCC 1.12482T?=?JCM 19469T). PMID:25985833

  8. A HIGHLY SELECTIVE PCR PROTOCOL FOR DETECTING 16S RRNA GENES OF THE GENUS PSEUDOMONAS (SENSU STRICTO) IN ENVIRONMENTAL SAMPLES

    EPA Science Inventory

    Pseudomonas species are plant, animal, and human pathogens; exhibit plant pathogen-suppressing properties useful in biological control; or express metabolic versatilities valued in biotechnology and bioremediation. Specific detection of Pseudomonas species in the environment may ...

  9. Characterization of the Substrate Specificity of PhlD, a Type III Polyketide Synthase from Pseudomonas fluorescens*S

    E-print Network

    Zhao, Huimin

    Pseudomonas fluorescens*S Received for publication,July 10, 2006, and in revised form, August 23, 2006 at Urbana-Champaign, Urbana, Illinois 61801 PhlD, a type III polyketide synthase from Pseudomonas fluo

  10. In Vitro Antibacterial Efficacy of 21 Indian Timber-Yielding Plants Against Multidrug-Resistant Bacteria Causing Urinary Tract Infection

    PubMed Central

    Mishra, Monali P.; Padhy, Rabindra N.

    2013-01-01

    Objectives To screen methanolic leaf extracts of 21 timber-yielding plants for antibacterial activity against nine species of uropathogenic bacteria isolated from clinical samples of a hospital (Enterococcus faecalis, Staphylococcus aureus, Acinetobacter baumannii, Citrobacter freundii, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa). Methods Bacterial strains were subjected to antibiotic sensitivity tests by the Kirby–Bauer's disc diffusion method. The antibacterial potentiality of leaf extracts was monitored by the agar-well diffusion method with multidrug-resistant (MDR) strains of nine uropathogens. Results Two Gram-positive isolates, E. faecalis and S. aureus, were resistant to 14 of the 18 antibiotics used. Gram-negative isolates A. baumannii, C. freundii, E. aerogenes, E. coli, K. pneumoniae, P. mirabilis, and P. aeruginosa were resistant to 10, 12, 9, 11, 11, 10, and 11 antibiotics, respectively, of the 14 antibiotics used. Methanolic leaf extracts of Anogeissus acuminata had the maximum zone of inhibition size—29 mm against S. aureus and 28 mm against E. faecalis and P. aeruginosa. Cassia tora had 29 mm as the zone of inhibition size for E. faecalis, E. aerogenes, and P. aeruginosa. Based on the minimum inhibitory concentration and minimum bactericidal concentration values, the most effective 10 plants against uropathogens could be arranged in decreasing order as follows: C. tora > A. acuminata > Schleichera oleosa > Pterocarpus santalinus > Eugenia jambolana > Bridelia retusa > Mimusops elengi > Stereospermum kunthianum > Tectona grandis > Anthocephalus cadamba. The following eight plants had moderate control capacity: Artocarpus heterophyllus, Azadirachta indica, Dalbergia latifolia, Eucalyptus citriodora, Gmelina arborea, Pongamia pinnata, Pterocarpus marsupium, and Shorea robusta. E. coli, followed by A. baumannii, C. freundii, E. aerogenes, P. mirabilis, and P. aeruginosa were controlled by higher amounts/levels of leaf extracts. Phytochemicals of all plants were qualitatively estimated. Conclusions A majority of timber-yielding plants studied had in vitro control capacity against MDR uropathogenic bacteria. PMID:24524024

  11. PilM/N/O/P Proteins Form an Inner Membrane Complex That Affects the Stability of the Pseudomonas aeruginosa

    E-print Network

    Burrows, Lori

    equivalent type IVa pilus complex. Using Pseudomonas aeruginosa as model organism, we found that all four reserved. Edited by I. Wilson Keywords: Pseudomonas aeruginosa; type IV pili; PilM/PilN/PilO/PilP; pilusPilM/N/O/P Proteins Form an Inner Membrane Complex That Affects the Stability of the Pseudomonas

  12. OccK Channels from Pseudomonas aeruginosa Exhibit Diverse Single-Channel Electrical Signatures but Conserved Anion Selectivity

    E-print Network

    Movileanu, Liviu

    OccK Channels from Pseudomonas aeruginosa Exhibit Diverse Single- Channel Electrical Signatures States *S Supporting Information ABSTRACT: Pseudomonas aeruginosa is a Gram-negative bacterium. Pseudomonas aeruginosa is a versatile human pathogen that exhibits a fundamental distinction from other Gram

  13. Functional Mapping of PilF and PilQ in the Pseudomonas aeruginosa Type IV Pilus System

    E-print Network

    Burrows, Lori

    Functional Mapping of PilF and PilQ in the Pseudomonas aeruginosa Type IV Pilus System Jason Koo ABSTRACT: Pseudomonas aeruginosa uses type IV pili (T4P) to interact with the environment and as key interactions between the organism and its environment.1-7 In many pathogens, including Pseudomonas aeruginosa

  14. 40 CFR 180.1200 - Pseudomonas fluorescens strain PRA-25; temporary exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pseudomonas fluorescens strain PRA-25... RESIDUES IN FOOD Exemptions From Tolerances § 180.1200 Pseudomonas fluorescens strain PRA-25; temporary... established for residues of the microbial pesticide, pseudomonas fluorescens strain PRA-25 when used on...

  15. 40 CFR 180.1212 - Pseudomonas chlororaphis Strain 63-28; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... 40 Protection of Environment 23 2010-07-01 2010-07-01 false Pseudomonas chlororaphis Strain 63-28... RESIDUES IN FOOD Exemptions From Tolerances § 180.1212 Pseudomonas chlororaphis Strain 63-28; exemption... for residues of the microbial pesticide Pseudomonas chlororaphis Strain 63-28 in or on all...

  16. The Cytoplasmic Heme-binding Protein (PhuS) from the Heme Uptake System of Pseudomonas aeruginosa Is an

    E-print Network

    Wraight, Colin A.

    The Cytoplasmic Heme-binding Protein (PhuS) from the Heme Uptake System of Pseudomonas aeruginosaS from the opportunistic pathogen Pseudomonas aeruginosa. Spectroscopic characterization of the heme). In the opportunistic pathogen Pseudomonas aeruginosa, two distinct heme uptake operons have been identified (10

  17. Histidine is a source of the anti-oxidant, -ketoglutarate in Pseudomonas fluorescens challenged by oxidative stress

    E-print Network

    Appanna, Vasu

    Histidine is a source of the anti-oxidant, -ketoglutarate in Pseudomonas fluorescens challenged metabolism in the model organism, Pseudomonas fluorescens challenged by hydrogen peroxide (H2O2). Here we ­ Pseudomonas fluorescens ­ glutamate dehydrogenase ­ metabolic reprogramming 2 #12;Introduction: Oxidative

  18. 40 CFR 180.1304 - Pseudomonas fluorescens strain CL145A; exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Pseudomonas fluorescens strain CL145A... RESIDUES IN FOOD Exemptions From Tolerances § 180.1304 Pseudomonas fluorescens strain CL145A; exemption... for residues of Pseudomonas fluorescens strain CL145A in or on all food commodities when applied as...

  19. 40 CFR 180.1200 - Pseudomonas fluorescens strain PRA-25; temporary exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... 40 Protection of Environment 25 2013-07-01 2013-07-01 false Pseudomonas fluorescens strain PRA-25... RESIDUES IN FOOD Exemptions From Tolerances § 180.1200 Pseudomonas fluorescens strain PRA-25; temporary... established for residues of the microbial pesticide, pseudomonas fluorescens strain PRA-25 when used on...

  20. 40 CFR 180.1200 - Pseudomonas fluorescens strain PRA-25; temporary exemption from the requirement of a tolerance.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... 40 Protection of Environment 24 2011-07-01 2011-07-01 false Pseudomonas fluorescens strain PRA-25... RESIDUES IN FOOD Exemptions From Tolerances § 180.1200 Pseudomonas fluorescens strain PRA-25; temporary... established for residues of the microbial pesticide, pseudomonas fluorescens strain PRA-25 when used on...

  1. MI66CH24-Burrows ARI 25 June 2012 8:39 Pseudomonas aeruginosa

    E-print Network

    Burrows, Lori

    MI66CH24-Burrows ARI 25 June 2012 8:39 R E V I E W S IN A D V A N CE Pseudomonas aeruginosa for DNA uptake, and pathogenesis. Pseudomonas aeruginosa has emerged as one of the key model systems findings concerning the three different types of T4P found in P. aeruginosa (type

  2. Regulation of Polyphosphate Kinase Production by Antisense RNA in Pseudomonas fluorescens Pf0-1

    PubMed Central

    Nicoll, Julie S.; Levy, Stuart B.

    2012-01-01

    Pseudomonas spp. adapt rapidly to environmental fluctuations. Loss or overproduction of polyphosphate reduces the fitness of Pseudomonas fluorescens Pf0-1, indicating the importance of the fine-tuning of polyphosphate production. An antisense RNA was investigated and shown to regulate the polyphosphate kinase gene (ppk) by a posttranscriptional mechanism reducing ppk transcript abundance. PMID:22492458

  3. The Pseudomonas aeruginosa multidrug efflux regulator MexR uses an oxidation-sensing mechanism

    E-print Network

    Dinner, Aaron

    The Pseudomonas aeruginosa multidrug efflux regulator MexR uses an oxidation-sensing mechanism Hao pathogen Pseudomonas aeruginosa. The mechanism of MexR-regulated antibiotic resistance has never been. aeruginosa. antibiotic resistance regulation thiol modification MarR oxidative stress transcription

  4. 40 CFR 180.1261 - Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages.

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... and Pseudomonas syringae pv. tomato specific Bacteriophages. 180.1261 Section 180.1261 Protection of.... vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages. An exemption from the requirement of a tolerance is established for residues of Xanthomonas campestris pv. vesicatoria and...

  5. Anlisis de Pseudomonas Fitopatgenas Usando Mtodos Inteligentes de Aprendizaje: Un Enfoque General Sobre

    E-print Network

    Gent, Universiteit

    :1-16. Abstract. The identification of plant-pathogenic bacteria is often of high importance. In this paper, we General Sobre Taxonomía y Análisis de Ácidos Grasos Dentro del Género Pseudomonas Analysis of Plant-Pathogenic. 2010. Análysis of plant-pathogenic pseudomonas species using intelligent learning methods: A general

  6. Redundant phenazine operons in Pseudomonas aeruginosa exhibit environment-dependent expression

    E-print Network

    Dietrich, Lars

    Redundant phenazine operons in Pseudomonas aeruginosa exhibit environment-dependent expression redundant operons in the adaptable bacte- rium Pseudomonas aeruginosa PA14. These operons, phzA1-G1 (phz1. aeruginosa physiology and act as virulence factors during opportunistic infections of plant and animal hosts

  7. The Carbon Monoxide Releasing Molecule CORM-2 Attenuates Pseudomonas aeruginosa Biofilm Formation

    E-print Network

    Dietrich, Lars

    The Carbon Monoxide Releasing Molecule CORM-2 Attenuates Pseudomonas aeruginosa Biofilm Formation surface-associated growth of the Gram-negative pathogen Pseudomonas aeruginosa by both preventing biofilm combined with tobramycin, a drug commonly used to treat P. aeruginosa lung infections. CORM-2 inhibited

  8. Crystal Structure and RNA Binding of the Tex Protein from Pseudomonas aeruginosa

    E-print Network

    Hill, Chris

    Crystal Structure and RNA Binding of the Tex Protein from Pseudomonas aeruginosa Sean J. Johnson1 of the 86-kDa Tex protein from Pseudomonas aeruginosa at 2.3 and 2.5 Ĺ resolution, respectively. aeruginosa (PA5201) appears to play an important role in pathogenesis, being required for lung infection

  9. Attenuation of Pseudomonas aeruginosa virulence by medicinal plants in a Caenorhabditis elegans

    E-print Network

    Ausubel, Frederick M.

    Attenuation of Pseudomonas aeruginosa virulence by medicinal plants in a Caenorhabditis elegans factors and innate antibiotic resistance enables the opportunistic human pathogen Pseudomonas aeruginosa were evaluated in toxin-based and infection-based assays using P. aeruginosa strains PAO1 and PA14

  10. 40 CFR 180.1261 - Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages.

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... and Pseudomonas syringae pv. tomato specific Bacteriophages. 180.1261 Section 180.1261 Protection of.... vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages. An exemption from the requirement of... syringae pv. tomato specific bacteriophages in or on pepper and tomato....

  11. 40 CFR 180.1261 - Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages.

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... and Pseudomonas syringae pv. tomato specific Bacteriophages. 180.1261 Section 180.1261 Protection of.... vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages. An exemption from the requirement of... syringae pv. tomato specific bacteriophages in or on pepper and tomato....

  12. 40 CFR 180.1261 - Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages.

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... and Pseudomonas syringae pv. tomato specific Bacteriophages. 180.1261 Section 180.1261 Protection of.... vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages. An exemption from the requirement of... syringae pv. tomato specific bacteriophages in or on pepper and tomato....

  13. 40 CFR 180.1261 - Xanthomonas campestris pv. vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages.

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... and Pseudomonas syringae pv. tomato specific Bacteriophages. 180.1261 Section 180.1261 Protection of.... vesicatoria and Pseudomonas syringae pv. tomato specific Bacteriophages. An exemption from the requirement of... syringae pv. tomato specific bacteriophages in or on pepper and tomato....

  14. An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants

    E-print Network

    Ausubel, Frederick M.

    An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants of the opportunistic human pathogen Pseudomonas aeruginosa strain PA14, we constructed a nonredundant library of PA14 transposon mutants (the PA14NR Set) in which nonessential PA14 genes are represented by a single transposon

  15. Genomic Analysis of Secondary Metabolite Production by Pseudomonas fluorescens Pf-5

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The complete genomic sequences of several Pseudomonas spp. that live in a commensal relationship with plants are now available. Among these is the biological control bacterium Pseudomonas fluorescens Pf-5. Nearly 6% of the 7.07 Mb genome of Pf-5 is devoted to the biosynthesis of secondary metaboli...

  16. pA506: A conjugative plasmid of the plant epiphyte Pseudomonas fluorescens A506

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas fluorescens A506 is an plant-epiphytic bacterium that is used commercially in the United States for the biological control of fire blight disease of pear and apple. Here, we demonstrate that A506 has a 57 kB conjugative plasmid that can transfer to other strains of Pseudomonas spp. and ...

  17. Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone.

    PubMed

    Luczkiewicz, Aneta; Kotlarska, Ewa; Artichowicz, Wojciech; Tarasewicz, Katarzyna; Fudala-Ksiazek, Sylwia

    2015-12-01

    In this study, species distribution and antimicrobial susceptibility of cultivated Pseudomonas spp. were studied in influent (INF), effluent (EFF), and marine outfall (MOut) of wastewater treatment plant (WWTP). The susceptibility was tested against 8 antimicrobial classes, active against Pseudomonas spp.: aminoglycosides, carbapenems, broad-spectrum cephalosporins from the 3rd and 4th generation, extended-spectrum penicillins, as well as their combination with the ?-lactamase inhibitors, monobactams, fluoroquinolones, and polymyxins. Among identified species, resistance to all antimicrobials but colistin was shown by Pseudomonas putida, the predominant species in all sampling points. In other species, resistance was observed mainly against ceftazidime, ticarcillin, ticarcillin-clavulanate, and aztreonam, although some isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, Pseudomonas pseudoalcaligenes, and Pseudomonas protegens showed multidrug-resistance (MDR) phenotype. Among P. putida, resistance to ?-lactams and to fluoroquinolones as well as multidrug resistance become more prevalent after wastewater treatment, but the resistance rate decreased in marine water samples. Obtained data, however, suggests that Pseudomonas spp. are equipped or are able to acquire a wide range of antibiotic resistance mechanisms, and thus should be monitored as possible source of resistance genes. PMID:26286796

  18. Identification of Pseudomonas cannabina pv. alisalensis previously isolated from diseased crucifers in Australia.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pseudomonas cannabina pv. alisalensis causes bacterial blight on crucifers, a severe disease which can reduce crucifer yields and result in economic losses in the US. Prior to the late 1990s P. cannabina pv. alisalensis was not distinguished from the pepper spot pathogen of crucifers, Pseudomonas s...

  19. Crystal structure of carboxylesterase from Pseudomonas fluorescens, an a/b hydrolase with broad substrate specificity

    E-print Network

    Suh, Se Won

    Crystal structure of carboxylesterase from Pseudomonas fluorescens, an a/b hydrolase with broad synthesis and hydrolysis of esters. One such carboxylesterase from Pseudomonas fluorescens is a homodimeric of this carboxylesterase. Results: In this study, the crystal structure of carboxylesterase from P. fluorescens has been

  20. vol. 166, no. 4 the american naturalist october 2005 Experimental Evolution of Pseudomonas fluorescens

    E-print Network

    Barrett, Rowan D.H.

    replicate lines of the bacterium Pseudomonas fluorescens in environments of different complexity by usingvol. 166, no. 4 the american naturalist october 2005 Experimental Evolution of Pseudomonas fluorescens in Simple and Complex Environments Rowan D. H. Barrett,1,* R. Craig MacLean,1,2, and Graham Bell1

  1. Testing temperature-induced proteomic changes in the plant-associated bacterium Pseudomonas

    E-print Network

    of P. fluorescens to colonize the plant environment. Introduction The plant-colonizing PseudomonasTesting temperature-induced proteomic changes in the plant-associated bacterium Pseudomonas fluorescens SBW25emi4_102 396..402 C. G. Knight,1,2,3 * X. X. Zhang,1,4,5 A. Gunn,4 T. Brenner,3 R. W. Jackson

  2. Evidences of non-reactive mercuryselenium compounds generated from cultures of Pseudomonas fluorescens

    E-print Network

    Belzile, Nelson

    chemically inert mercury­selenium (Hg­Se) compounds formed in a culture of Pseudomonas fluorescens exposedEvidences of non-reactive mercury­selenium compounds generated from cultures of Pseudomonas fluorescens Dan-Yi Yang a,b , Yu-Wei Chen a , Nelson Belzile a,c, a Department of Chemistry and Biochemistry

  3. Genomic diversity of Pseudomonas spp. isolated from aerial or root surfaces of plants

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Among the diverse strains of Pseudomonas fluorescens and Pseudomonas chlororaphis inhabiting plant surfaces are those that protect plants from infection by pathogens. To explore the diversity of these bacteria, we derived genomic sequences of seven strains that suppress plant disease. Along with t...

  4. Degradation of 3-phenylbutyric acid by Pseudomonas sp.

    PubMed Central

    Sariaslani, F S; Sudmeier, J L; Focht, D D

    1982-01-01

    Pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-PB) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. During early exponential growth, a catechol substance identified as 3-(2,3-dihydroxyphenyl)butyrate (2,3-DHPB) and its meta-cleavage product 2-hydroxy-7-methyl-6-oxononadioic-2,4-dienoic acid were produced. These products disappeared during late exponential growth, and considerable amounts of 2,3-DHPB reacted to form brownish polymeric substances. The catechol intermediate 2,3-DHPB could not be isolated, but cell-free extracts were able only to oxidize 3-(2,3-dihydroxyphenyl)propionate of all dihydroxy aromatic acids tested. Moreover, a reaction product caused by dehydration of 2,3-DHPB on silica gel was isolated and identified by spectral analysis as (--)-8-hydroxy-4-methyl-3,4-dihydrocoumarin. 3-Phenylpropionate and a hydroxycinnamate were found in supernatants of cultures grown on 3-PB; phenylacetate and benzoate were found in supernatants of cultures grown on 3-phenylpropionate; and phenylacetate was found in cultures grown on cinnamate. Cells grown on 3-PB rapidly oxidized 3-phenylpropionate, cinnamate, catechol, and 3-(2,3-dihydroxyphenyl)propionate, whereas 2-phenylpropionate, 2,3-dihydroxycinnamate, benzoate, phenylacetate, and salicylate were oxidized at much slower rates. Phenylsuccinate was not utilized for growth nor was it oxidized by washed cell suspensions grown on 3-PB. However, dual axenic cultures of Pseudomonas acidovorans and Klebsiella pneumoniae, which could not grow on phenylsuccinate alone, could grow syntrophically and produced the same metabolites found during catabolism of 3-PB by Pseudomonas sp. Washed cell suspensions of dual axenic cultures also immediately oxidized phenylsuccinate, 3-phenylpropionate, cinnamate, phenylacetate, and benzoate. PMID:7118830

  5. 21 CFR 522.90a - Ampicillin trihydrate sterile suspension.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ...bacterial enteritis caused by Escherichia coli and bacterial pneumonia caused by Pasteurella...respiratory tract infections due to E. coli, Pseudomonas spp., Proteus spp...Streptococcus spp.; tonsillitis due to E. coli, Pseudomonas spp., Streptococcus...

  6. Computer Simulation of the Rough Lipopolysaccharide Membrane of Pseudomonas Aeruginosa

    SciTech Connect

    Lins, Roberto D.; Straatsma, TP

    2001-08-01

    Lipopolysaccharides (LPS) form the major constituent of the outer membrane of Gram-negative bacteria, and are believed to play a key role in processes that govern microbial metal binding, microbial adsorption to mineral surfaces, and microbe mediated oxidation/reduction reactions at the bacterial exterior surface. A computational modeling capability is being developed for the study of geochemical reactions at the outer bacterial envelope of Gram-negative bacteria. The understanding of these mechanisms is crucial for the development of successful environmental bioremediation strategies. A molecular model for the rough LPS of Pseudomonas aeruginosa has been designed based on available experimentally determined structural information.

  7. Phlebovirus meningoencephalis complicated by Pseudomonas aeruginosa pneumonia: a case report.

    PubMed

    Anagnostou, Vassiliki; Sdouga, Maria; Volakli, Hellen; Violaki, Asimina; Papa, Anna

    2011-05-01

    In June 2004 an 8-year-old boy was admitted to a hospital in Thessaloniki, Greece, because of high fever, tachypnea, hypotonia, diarrhea, and tonoclonic convulsions. Phlebovirus infection was diagnosed by IgG seroconversion to Toscana virus. As IgM antibodies were not detected, it is suggested that this was an acute infection caused by a phlebovirus virus distinct from Toscana virus. Complication by a hospital-acquired Pseudomonas aeruginosa pneumonia resulted in 2 months of hospitalization. Slight ataxia was still present on discharge. PMID:20575643

  8. Fatal suppurative nephritis caused by Pseudomonas in a chimpanzee

    USGS Publications Warehouse

    Migaki, G.; Asher, D.M.; Casey, H.W.; Locke, L.N.; Gibbs, C.J.; Gajdusek, C.

    1979-01-01

    Reports of nephritis in chimpanzees are relatively rare, compared with those in other nonhuman primates. McClure and Guilloud reported chronic pyelonephritis in a 35-year-old female chimpanzee; Schmidt and Butler reported glomerulonephritis in an 11-year-old female chimpanzee, and Kim reported on a 12-year-old male with subacute interstitial nephritis in a chimpanzee after the animal had recurrent hemolysis due to phenolic intoxication. The present report deals with supprative nephritis caused by Pseudomonas resulting in renal failure in a chimpanzee.

  9. Phosphorylated tyrosine in the flagellum filament protein of Pseudomonas aeruginosa

    SciTech Connect

    Kelly-Wintenberg, K.; Anderson, T.; Montie, T.C. )

    1990-09-01

    Purified flagella from two strains of {sup 32}P-labeled Pseudomonas aeruginosa were shown to be phosphorylated. This was confirmed by autoradiography of flagellin protein in polyacrylamide gels. Thin-layer electrophoresis and autoradiography of flagellin partial hydrolysates indicated that phosphotyrosine was the major phosphorylated amino acid. High-pressure liquid chromatographic analysis confirmed the presence of phosphotyrosine in flagellum filament protein. Preliminary data indicated that less than one tyrosine per subunit was phosphorylated. No evidence was found for phosphorylation of serine or threonine. A function related to tyrosine phosphorylation has not been determined.

  10. [Modified procedure for pyocin typing of Pseudomonas aeruginosa (author's transl)].

    PubMed

    Bauernfeind, A; Petermüller, C; Burrows, J R

    1978-04-01

    The Procedure for pyocin typing of Pseudomonas aeruginosa isolations as proposed by GILLIES and GOVAN has been modified in the following aspects: strains are arranged in a symmetrical way, with a central producer zone and radial indicator streaks, optimal size of inocula for both producer (300 x 10(6)/ml) and indicators (50 X 10(6)/ml) was determined, unsupplemented Tryptic Soy Agar is used giving identical results to those when TSA is supplemented with 5% horse blood. The typing results obtained with this simplified and standardized procedure were identical with those from the cross streak method. PMID:418604

  11. Phosphorylated tyrosine in the flagellum filament protein of Pseudomonas aeruginosa.

    PubMed Central

    Kelly-Wintenberg, K; Anderson, T; Montie, T C

    1990-01-01

    Purified flagella from two strains of 32P-labeled Pseudomonas aeruginosa were shown to be phosphorylated. This was confirmed by autoradiography of flagellin protein in polyacrylamide gels. Thin-layer electrophoresis and autoradiography of flagellin partial hydrolysates indicated that phosphotyrosine was the major phosphorylated amino acid. High-pressure liquid chromatographic analysis confirmed the presence of phosphotyrosine in flagellum filament protein. Preliminary data indicated that less than one tyrosine per subunit was phosphorylated. No evidence was found for phosphorylation of serine or threonine. A function related to tyrosine phosphorylation has not been determined. Images PMID:1697577

  12. Regulation of phytotoxin production in Pseudomonas syringae pv. tabaci

    SciTech Connect

    Weaver, L.M.; Ghosh, S.; Knight, T.J.; Unkefer, P.J. )

    1991-05-01

    Pseudomonas syringae pv. tabaci, a pathogen of tobacco, is capable of colonizing the rhizosphere of many plants. This pathogen excretes tabtoxinine-{beta}-lactam (T{beta}L), an active site directed, irreversible inhibitor of glutamine synthetase. T{beta}L is produced in planta, in the rhizosphere, and under certain culture conditions. However, the factors which regulated T{beta}L production in these environments are unknown. As a first step in characterizing T{beta}L synthesis by P. syringae pv. tabaci, the authors have determined the effects of root exudates and various nutrients on production of T{beta}L by P. syringae pv. tabaci PT113.

  13. Nanoindentation of Pseudomonas aeruginosa bacterial biofilm using atomic force microscopy

    NASA Astrophysics Data System (ADS)

    Baniasadi, Mahmoud; Xu, Zhe; Gandee, Leah; Du, Yingjie; Lu, Hongbing; Zimmern, Philippe; Minary-Jolandan, Majid

    2014-12-01

    Bacterial biofilms are a source of many chronic infections. Biofilms and their inherent resistance to antibiotics are attributable to a range of health issues including affecting prosthetic implants, hospital-acquired infections, and wound infection. Mechanical properties of biofilm, in particular, at micro- and nano-scales, are governed by microstructures and porosity of the biofilm, which in turn may contribute to their inherent antibiotic resistance. We utilize atomic force microscopy (AFM)-based nanoindentation and finite element simulation to investigate the nanoscale mechanical properties of Pseudomonas aeruginosa bacterial biofilm. This biofilm was derived from human samples and represents a medically relevant model.

  14. Mitogenic effects of purified outer membrane proteins from Pseudomonas aeruginosa.

    PubMed Central

    Chen, Y H; Hancock, R E; Mishell, R I

    1980-01-01

    Three major outer membrane proteins from Pseudomonas aeruginosa PAO1 were purified and tested for their ability to stimulate resting murine lymphocytes to proliferate. It was demonstrated that picomole amounts of all three proteins were mitogenic for both intact and T-lymphocyte-depleted populations of spleen cells from C3H/HeJ mice. In contrast, they had no activity against either mature or immature thymocytes. Since the strain of mice used is unable to respond to lipopolysaccharide, we condlude that the three proteins are B-cell mitogens. Images Fig. 2 PMID:6769818

  15. Methods of detecting and controlling mucoid Pseudomonas biofilm production

    NASA Technical Reports Server (NTRS)

    Yu, Hongwei D. (Inventor); Qiu, Dongru (Inventor)

    2013-01-01

    Compositions and methods for detecting and controlling the conversion to mucoidy in Pseudomonas aeruginosa are disclosed. The present invention provides for detecting the switch from nonmucoid to mucoid state of P. aeruginosa by measuring mucE expression or MucE protein levels. The interaction between MucE and AlgW controls the switch to mucoidy in wild type P. aeruginosa. Also disclosed is an alginate biosynthesis heterologous expression system for use in screening candidate substances that inhibit conversion to mucoidy.

  16. HOPM1 mediated disease resistance to Pseudomonas syringae in Arabidopsis

    DOEpatents

    He, Sheng Yang (Okemos, MI); Nomura, Kinya (East Lansing, MI)

    2011-11-15

    The present invention relates to compositions and methods for enhancing plant defenses against pathogens. More particularly, the invention relates to enhancing plant immunity against bacterial pathogens, wherein HopM1.sub.1-300 mediated protection is enhanced, such as increased protection to Pseudomonas syringae pv. tomato DC3000 HopM1 and/or there is an increase in activity of an ATMIN associated plant protection protein, such as ATMIN7. Reagents of the present invention further provide a means of studying cellular trafficking while formulations of the present inventions provide increased pathogen resistance in plants.

  17. Factors Affecting Zebra Mussel Kill by the Bacterium Pseudomonas fluorescens

    SciTech Connect

    Daniel P. Molloy

    2004-02-24

    The specific purpose of this research project was to identify factors that affect zebra mussel kill by the bacterium Pseudomonas fluorescens. Test results obtained during this three-year project identified the following key variables as affecting mussel kill: treatment concentration, treatment duration, mussel siphoning activity, dissolved oxygen concentration, water temperature, and naturally suspended particle load. Using this latter information, the project culminated in a series of pipe tests which achieved high mussel kill inside power plants under once-through conditions using service water in artificial pipes.

  18. Crystal Structure of the Pseudomonas aeruginosa Virulence Factor Regulator ?†

    PubMed Central

    Cordes, Timothy J.; Worzalla, Gregory A.; Ginster, Aaron M.; Forest, Katrina T.

    2011-01-01

    Virulence factor regulator (Vfr) enhances Pseudomonas aeruginosa pathogenicity through its role as a global transcriptional regulator. The crystal structure of Vfr shows that it is a winged-helix DNA-binding protein like its homologue cyclic AMP receptor protein (CRP). In addition to an expected primary cyclic AMP-binding site, a second ligand-binding site is nestled between the N-terminal domain and the C-terminal helix-turn-helix domain. Unlike CRP, Vfr is a symmetric dimer in the absence of DNA. Removal of seven disordered N-terminal residues of Vfr prevents the growth of P. aeruginosa. PMID:21665969

  19. Monitoring for genetically engineered pseudomonas species in monterey county

    SciTech Connect

    Supkoff, D.; Opgenorth, D.; Lai, C.; Segawa, R.; Koehler, D.

    1987-01-01

    A field monitoring study was conducted to determine if genetically altered Pseudomonas fluorescens or P. syringae had been applied to sites in Monterey County. A series of diagnostic tests for antibiotic resistance, fluorescence ability, oxidase and arginine dihydrolase activities, hypersensitivity reaction and ice nucleation ability were conducted to screen bacteria isolated from field and control samples. No bacteria were detected from field samples which matched the expected test profiles of genetically altered bacterial products. In contrast, bacteria were consistently isolated from positive control samples with the expected characteristics of genetically altered bacteria.

  20. Crystal Structure of the Pseudomonas aeruginosa Virulence Factor Regulator

    SciTech Connect

    Cordes, Timothy J.; Worzalla, Gregory A.; Ginster, Aaron M.; Forest, Katrina T.

    2012-09-07

    Virulence factor regulator (Vfr) enhances Pseudomonas aeruginosa pathogenicity through its role as a global transcriptional regulator. The crystal structure of Vfr shows that it is a winged-helix DNA-binding protein like its homologue cyclic AMP receptor protein (CRP). In addition to an expected primary cyclic AMP-binding site, a second ligand-binding site is nestled between the N-terminal domain and the C-terminal helix-turn-helix domain. Unlike CRP, Vfr is a symmetric dimer in the absence of DNA. Removal of seven disordered N-terminal residues of Vfr prvents the growth of P. aeruginosa.