Science.gov

Sample records for real product alternative

  1. Reduction of the excess sludge production by biological alternating process: real application results and metabolic uncoupling mechanism.

    PubMed

    Eusebi, Anna Laura; Battistoni, Paolo

    2015-01-01

    The biological solution proposed to reduce the wasted sludge production is based on a process of alternating phases realized in a specific reactor (alternate cycles in sludge line (ACSL)) where a quote of the recycle sludge is treated and sent back to the main activated sludge process. The ACSL process was applied in two urban wastewater treatment plants (WWTPs). The reduction was tested by changing the hydraulic retention time and the conditions of oxidation reduction potential. The main mechanism of the process is recognized in the metabolic uncoupling. In fact, an increase in the specific oxygen uptake rate in the biological reactors was recorded (up to 20 mg/g VSS/h), which was stimulated by the fasting condition in the ACSL. The process is able to reduce the observed sludge yield on average of 25-30% with final average values reaching 0.179 kg VSS/kg chemical oxygen demand (COD) for WWTP1 and 0.117 kg VSS/kg COD for WWTP2. PMID:25413108

  2. REAL-TIME CONTROL (RTC) - EVALUATION OF ALTERNATIVES

    EPA Science Inventory

    The studies were performed at real demonstration sites. The evaluations of RTC alternatives were conducted on portions of sewerage systems near Paris, France and in Quebec City, Canada, respectively. RTC alternatives use dynamic mathematical modeling and simulations to develop ...

  3. MISR Level 1 Near Real Time Products

    Atmospheric Science Data Center

    2014-09-15

    Level 1 Near Real Time The MISR Near Real Time Level 1 data products consist of radiance measurements organized in 10-50 minute ... (off-nadir) cameras. The remaining channels are sampled at 1.1 km. ...

  4. Survey of alternative feedstocks for biodiesel production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Summarized will be results obtained from the production of biodiesel from several alternative feedstocks with promising agronomic characteristics. Such feedstocks include camelina (Camelina sativa L.), coriander (Coriandrum sativum L.), field pennycress (Thlaspi arvense L.), and meadowfoam (Limnanth...

  5. A Survey of Alternative Oxygen Production Technologies

    NASA Technical Reports Server (NTRS)

    Lueck, Dale E.; Parrish, Clyde F.; Buttner, William J.; Surma, Jan M.; Delgado, H. (Technical Monitor)

    2000-01-01

    Utilization of the Martian atmosphere for the production of fuel and oxygen has been extensively studied. The baseline fuel production process is a Sabatier reactor, which produces methane and water from carbon dioxide and hydrogen. The oxygen produced from the electrolysis of the water is only half of that needed for methane-based rocket propellant, and additional oxygen is needed for breathing air, fuel cells and other energy sources. Zirconia electrolysis cells for the direct reduction of CO2 are being developed as an alternative means of producing oxygen, but present many challenges for a large-scale oxygen production system. The very high operating temperatures and fragile nature of the cells coupled with fairly high operating voltages leave room for improvement. This paper will survey alternative oxygen production technologies, present data on operating characteristics, materials of construction, and some preliminary laboratory results on attempts to implement each.

  6. Available Alternative Fuel School Bus Products--2004

    SciTech Connect

    Not Available

    2004-04-01

    This 4-page Clean Cities fact sheet provides a list of the currently available (and soon to be available) model year 2004 alternative fuel school bus and school bus engine products. It includes information from Blue Bird Corporation, Collins Bus Corporation, Corbeil Bus, Ford Motor Company, General Motors Corporation, Thomas Built Buses, Inc., Clean Air Partners, Cummins Westport, and Deere & Company.

  7. Alternative indicators for measuring hospital productivity.

    PubMed

    Serway, G D; Strum, D W; Haug, W F

    1987-08-01

    This article explores the premise that the appropriateness and usefulness of typical hospital productivity measures have been affected by three changes in delivery: Organizational restructuring and other definition and data source changes that make full-time equivalent employee (FTE) measurements ambiguous. Transition to prospective payment (diagnosis-related groups). Increase in capitation (prepaid, at risk) programs. The effects of these changes on productivity management indicate the need for alternative productivity indicators. Several productivity measures that complement these changes in internal operations and the external hospital business environment are presented. These are based on an analysis of four hospitals within a multihospital system, and an illustration and interpretation of an array of measures, based on ten months of actual data, is provided. In conclusion, the recommendation is made for hospital management to collect an expanded set of productivity measures and review them in light of changing expense and revenue management schemes inherent in new payment modes. PMID:10312194

  8. A Survey of Alternative Oxygen Production Technologies

    NASA Technical Reports Server (NTRS)

    Lueck, Dale E.; Parrish, Clyde F.; Buttner, William J.; Surma, Jan M.; Delgado, H. (Technical Monitor)

    2001-01-01

    Utilization of the Martian atmosphere for the production of fuel and oxygen has been extensively studied. The baseline fuel production process is a Sabatier reactor, which produces methane and water from carbon dioxide and hydrogen. The oxygen produced from the electrolysis of the water is only half of that needed for methane-based rocket propellant, and additional oxygen is needed for breathing air, fuel cells and other energy sources. Zirconia electrolysis cells for the direct reduction of CO2 arc being developed as an alternative means of producing oxygen, but present many challenges for a large-scale oxygen production system. The very high operating temperatures and fragile nature of the cells coupled with fairly high operating voltages leave room for improvement. This paper will survey alternative oxygen production technologies, present data on operating characteristics, materials of construction, and some preliminary laboratory results on attempts to implement each. Our goal is to significantly improve upon the characteristics of proposed zirconia cells for oxygen production. To achieve that goal we are looking at electrolytic systems that operate at significantly lower temperatures, preferably below 31C to allow the incorporation of liquid CO2 in the electrolyte. Our preliminary results indicate that such a system will have much higher current densities and have simpler cathode construction than a porous gas feed electrode system. Such a system could be achieved based on nonaqueous electrolytes or ionic liquids. We are focusing our research on the anode reaction that will produce oxygen from a product generated at the cathode using CO2 as the feed. Operation at low temperatures also will open up the full range of polymer and metal materials, allowing a more robust system design to withstand the rigors of flight, landing, and long term unattended operation on the surface of Mars.

  9. The Real-World Colour of the Dative Alternation.

    ERIC Educational Resources Information Center

    Groefsema, Marjolein

    2001-01-01

    Challenges assumptions regarding dative alternation and proposes an account in terms of one general constraint of what makes a verb a possible verb, which operates over verb-specific conceptual information. Central to the proposal is the assumption that the different forms of dative verbs do not only encode different conceptual representations of…

  10. Alternative delivery of male accessory gland products

    PubMed Central

    2014-01-01

    To increase fertilization success, males transfer accessory gland products (Acps). Several species have evolved unconventional Acps transfer modes, meaning that Acps are transferred separately from the sperm. By surveying the sperm-free Acps transfer cases, we show that these animals have evolved a common strategy to deliver Acps: they all inject Acps directly through the partner’s body wall into the hemolymph. Our review of this mode of Acps transfer reveals another striking similarity: they all transfer sperm in packages or via the skin, which may leave little room for Acps transfer via the conventional route in seminal fluid. We synthesise the knowledge about the function, and the effects in the recipients, of the Acps found in the widely diverse taxa (including earthworms, sea slugs, terrestrial snails, scorpions and salamanders) that inject these substances. Despite the clearly independent evolution of the injection devices, these animals have evolved a common alternative strategy to get their partners to accept and/or use their sperm. Most importantly, the evolution of the injection devices for the delivery of Acps highlights how the latter are pivotal for male reproductive success and, hence, strongly influence sexual selection. PMID:24708537

  11. Ovarian cancer standard of care: are there real alternatives?

    PubMed Central

    Pepa, Chiara Della; Tonini, Giuseppe; Pisano, Carmela; Di Napoli, Marilena; Cecere, Sabrina Chiara; Tambaro, Rosa; Facchini, Gaetano; Pignata, Sando

    2015-01-01

    Ovarian cancer remains a major issue for gynecological oncologists, and most patients are diagnosed when the disease is already advanced with a poor chance of survival. Debulking surgery followed by platinum-taxane chemotherapy is the current standard of care, but based on several different strategies currently under evaluation, some encouraging data have been published in the last 4 to 5 years. This review provides a state-of-the-art overview of the available alternatives to conventional treatment and the most promising new combinations. For example, neoadjuvant chemotherapy does not seem to be inferior to primary debulking. Despite its outcome improvements, intraperitoneal chemotherapy struggles for acceptance due to the heavy toxicity. Dose-dense chemotherapy, after showing an impressive efficacy in Asian populations, has not produced equal results in a European cohort, and the results of alternative platinum doublets are not superior to those of carboplatin and paclitaxel. In this setting, adherence to a maintenance therapy after first-line treatment and multiple (primarily antiangiogenic) agents appears to be effective. Although many questions, including the duration of maintenance treatment and the use of bevacizumab beyond progression, remain unanswered, new biologic agents, such as poly(ADP-ribose) polymerase (PARP) inhibitors, nintedanib, and mitogen-activated protein/extracellular signal-regulated kinase (MEK) inhibitors, have emerged as potential therapeutic options in the very near future. Based on the multiplicity of available strategies, the histological and molecular features of the tumor, in addition to patient's clinical condition and disease state, continue to gain importance in guiding treatment choices. PMID:25556615

  12. Real-time data flow and product generating for GNSS

    NASA Technical Reports Server (NTRS)

    Muellerschoen, Ronald J.; Caissy, Mark

    2004-01-01

    The last IGS workshop with the theme 'Towards Real-Time' resulted in the design of a prototype for real-time data and sharing within the IGS. A prototype real-time network is being established that will serve as a test bed for real-time activities within the IGS. We review the developments of the prototype and discuss some of the existing methods and related products of real-time GNSS systems. Recommendations are made concerning real-time data distribution and product generation.

  13. DEVELOPING ALTERNATIVE POWER TO DRIVE A PARTITIONED AQUACULTURE SYSTEM FOR INTENSIVE FOOD FISH PRODUCTION

    EPA Science Inventory

    Data collected, such as fish production rates and construction/operating costs, will allow us to evaluate the predictions generated by our modeling activities as well as provide real estimates of cost/benefit for the application of alternative PAS technology in temperate water...

  14. Alternative Agricultural Enterprises. Production, Management & Marketing.

    ERIC Educational Resources Information Center

    Fox, Linda Kirk; And Others

    These nine cooperative extension bulletins provide basic information on various alternative agricultural enterprises. Discussed in the first eight bulletins are the following topics: business ownership (sole proprietorship, partnership, incorporation, cooperatives); business and the family (goals, qualifications, ways of ensuring family support,…

  15. Alternate feedstocks and technologies for biodiesel production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    U.S. biodiesel production is presently estimated at 800 million gallons annually, and this fuel is no longer a research curiosity - it is entering the nation’s fuel infrastructure. Some estimates are that production will reach nearly twice that value in the next 10 to 12 years. This would stress a...

  16. Real-Life Challenges in Using Augmentative and Alternative Communication by Persons with Amyotrophic Lateral Sclerosis

    ERIC Educational Resources Information Center

    Ray, Jayanti

    2015-01-01

    Given the linguistic and cognitive demands of communication, adult Augmentative and Alternative Communication (AAC) users with acquired communication disorders may have difficulty using AAC systems consistently and effectively in "real-life" situations. The process of recommending AAC systems and strategies is an area of exploration,…

  17. Production of Bacterial Cellulose from Alternate Feedstocks

    SciTech Connect

    Thompson, David Neil; Hamilton, Melinda Ann

    2000-05-01

    Production of bacterial cellulose by Acetobacter xylinum ATCC 10821 and 23770 in static cultures was tested from unamended food process effluents. Effluents included low- and high-solids potato effluents (LS & HS), cheese whey permeate (CW), and sugar beet raffinate (CSB). Strain 23770 produced 10% less cellulose from glucose than did 10821, and diverted more glucose to gluconate. Unamended HS, CW, and CSB were unsuitable for cellulose production by either strain, while LS was unsuitable for production by 10821. However, 23770 produced 17% more cellulose from LS than from glucose, indicating unamended LS could serve as a feedstock for bacterial cellulose.

  18. Production of bacterial cellulose from alternate feedstocks

    SciTech Connect

    D. N. Thompson; M. A. Hamilton

    2000-05-07

    Production of bacterial cellulose by Acetobacter xylinum ATCC 10821 and 23770 in static cultures was tested from unamended food process effluents. Effluents included low- and high-solids potato effluents (LS and HS), cheese whey permeate (CW), and sugar beet raffinate (CSB). Strain 23770 produced 10% less cellulose from glucose than did 10821, and diverted more glucose to gluconate. Unamended HS, CW, and CSB were unsuitable for cellulose production by either strain, while LS was unsuitable for production by 10821. However, 23770 produced 17% more cellulose from LS than from glucose, indicating unamended LS could serve as a feedstock for bacterial cellulose.

  19. New Alternatives in Seafood Restructured Products.

    PubMed

    Moreno, Helena M; Herranz, Beatriz; Pérez-Mateos, Miriam; Sánchez-Alonso, Isabel; Borderías, Javier A

    2016-01-01

    A general overview, focusing on new trends in the different techniques used in restructured seafood product processing has been described in this work. Heat-induced gelation has been more widely studied in scientific literature than cold gelation technology. This latter technology includes the use of hydrocolloids (alginates and glucomannan) or enzymes (microbial transglutaminase) for making both raw and cooked restructured products. In restructuration processes, fortification processing with some functional ingredients is studied, giving as a result extra value to the products as well as increasing the variety of new seafood products. The process of alleviating heavy metals and organic pollutants from the raw material used has also been reviewed in the present paper. PMID:25000341

  20. Alternative Bench Standards: Sample Production Report

    SciTech Connect

    N. R. Mann; T. P. Houghton; M. G. Watrous; J. G. Eisenmenger; R. K. Hague

    2012-09-01

    The INL has prepared four standards representing krypton concentrations of 1.1X, 1.54X, 10X and 100X the reported atmospheric value of 70 dpm 85Kr per cubic centimeter of Kr gas at 25 degrees C (ie. 1.1X is 1.1 x 70, or 77 dpm 85Kr per cubic centimeter of Kr gas at 25 degrees C). A t-zero date and time of January 1, 2012 at 1200 Zulu was used for all standards. The Alternative Bench Standards (ABS) of 1.1X, 1.54X, 10X and 100X, are designated by titles of ABS-A, ABS-B, ABS C and ABS-D, respectively. The concentration of Kr in air is 1.14 ppm.

  1. MISR Near Real Time Products Available

    Atmospheric Science Data Center

    2014-09-04

    ... BUFR Format .   The MISR NRT product offerings, beginning from September 1, 2014 are available via the ASDC by subscription ... MISR_NRT , and by order through the online NASA Reverb Earth Science Discovery Tool .   Information about these products and ...

  2. GATEWAY Demonstrations: Exploring SSL Product Performance in the Real World

    SciTech Connect

    2013-10-01

    Fact sheet that outlines DOE's GATEWAY technology demonstration program, which evaluates high-performance SSL products for general illumination in a variety of real-world exterior and interior applications.

  3. [Research progress of alternative production approaches of salidroside].

    PubMed

    Wu, Xiu-Wen; Peng, Yu-Shuai; Wang, Ru-Feng

    2013-11-01

    Salidroside, one of the active components of Rhodiola plants, is a phenolic glycoside with significant biological activities. The investigation and development of alternative production approaches of salidroside is of high academic and application values due to the limited resource of Rhodiola plants, and from which the low yield of salidroside. This review summarized the research progress and perspective of the alternative production approaches of salidroside including both chemosynthetic and biosynthetic methods and pathways. PMID:24494549

  4. Observation of jet production by real photons

    NASA Astrophysics Data System (ADS)

    Adams, D.; Ahmad, S.; Akchurin, N.; Birmingham, P.; Breuer, H.; Chang, C. C.; Cihangir, S.; Corcoran, M. D.; Davis, W. L.; Gustafson, H. R.; Holmgren, H.; Kasper, P.; Kruk, J.; Lincoln, D.; Longo, M. J.; Marraffino, J.; McPherson, J.; Miettinen, H. E.; Morrow, G.; Mutchler, G. S.; Naples, D.; Onel, Y.; Skeens, J.; Thomas, G. P.; Traynor, M. M.; Waters, J. W.; Webster, M. S.; Xu, J. P.; Zhu, Q.

    1994-04-01

    Interactions of high energy photons on a hyrogen target have been studied using a large acceptance segmented calorimeter. The event topology clearly shows the production of dijet final states as predicted by perturbative QCD. The energy flow in the photon (forward) direction is compared both to Monte Carlo expectations and to that produced in πp interactions.

  5. MISR Level 2 Near Real Time Products

    Atmospheric Science Data Center

    2014-10-01

    ... in 10-50 minute sessions representing a portion of a single MISR orbit. Parameters are projected onto a Space-Oblique Mercator (SOM) ... include two formats of the MISR Level 2 Cloud Motion Vector (CMV) product, comprising height-resolved cloud motions representative ...

  6. An empirical approach for real-time earthquake damage assessement: an alternative of shake maps

    NASA Astrophysics Data System (ADS)

    Pirentis, A. P.; Halaris, Th.; Papadopoulos, G. A.

    2009-04-01

    The production of reliable shake maps in real time is not always possible, e.g. due to lack of adequate data or of trustable relations between ground acceleration and elements of the macroseismic field. Then, an alternative is proposed: the rapid production of the expected macroseismic field, within few minutes after focal parameters of an earthquake have been determined, on the basis of empirical relations between focal parameters and elements of the macroseismic field such as maximum seismic intensity, Im, and area, Si, defined by isoseismal of degree i. The purpose of this research initiative is the implementation of the results for early warning as regards the impact of strong earthquakes immediately after their occurrence. A data set of about 147 shallow, instrumental earthquakes that occurred in Greece from 1911 up to the end of 90's has been compiled and empirical relations were investigated between earthquake focal parameters on one hand and Im and Si on the other. The focal depth as well as the epicentral location are critical parameters in this procedure. However, reliable focal depth determinations are available only for the last 15 years or so. On the other hand, the geomorphological configuration of Greece makes the earthquake impact very sensitive to the epicentral distance. In fact, all the other parameters being equal the impact is quite different for inland, coastal and open-sea earthquake sources. For these reasons a simplified distance-from-the-coastline criterion is introduced and employed in order to classify the earthquakes in three distinct classes: inland, coastal and open sea, respectively. The data set compiled indicates that for strong earthquakes the meizoseismal extends at a radius of no more than 30 km from the epicentre. Therefore, the class of coastal earthquakes includes those having their epicentres at a distance of no more than 30 km from the closest coast. Then, for each one of the three classes of earthquake events simple linear

  7. Real-Time Data Use for Operational Space Weather Products

    NASA Astrophysics Data System (ADS)

    Quigley, S.; Nobis, T. E.

    2010-12-01

    The Space Vehicles Directorate of the Air Force Research Laboratory (AFRL/RVBX) and the Space Environment Division of the Space and Missile Systems Center (AFSPC SYAG/WMLE) have combined efforts to design, develop, test, implement, and validate numerical and graphical products for Air Force Space Command’s (AFSPC) Space Environmental Effects Fusion System (SEEFS). These products were developed to analyze, specify, and forecast the effects of the near-earth space environment on Department of Defense weapons, navigation, communications, and surveillance systems in real/near-real time. This real-time attribute is the primary factor in allowing for actual operational product output, but it’s also responsible for a variety of detrimental effects that need to be considered, researched, mitigated, or otherwise eliminated in future/upgrade product applications. This presentation will provide brief overviews of the SEEFS products, along with information and recommendations concerned with their near/real-time data acquisition and use, to include: input data requirements, inputs/outputs ownership, observation cadence, transmission/receipt links and cadence, data latency, quality control, error propagation and associated confidence level applications, and ensemble model run potentials. Validation issues related to real-time data will also be addressed, along with recommendations for new real-time data archiving that should prove operationally beneficial.

  8. Validation of an alternative microbiological method for tissue products.

    PubMed

    Suessner, Susanne; Hennerbichler, Simone; Schreiberhuber, Stefanie; Stuebl, Doris; Gabriel, Christian

    2014-06-01

    According to the European Pharmacopoeia sterility testing of products includes an incubation time of 14 days in thioglycollate medium and soya-bean casein medium. In this case a large period of time is needed for product testing. So we designed a study to evaluate an alternative method for sterility testing. The aim of this study was to reduce the incubation time for the routinely produced products in our tissue bank (cornea and amnion grafts) by obtaining the same detection limit, accurateness and recovery rates as the reference method described in the European Pharmacopoeia. The study included two steps of validation. Primary validation compared the reference method with the alternative method. Therefore eight bacterial and two fungi test strains were tested at their preferred milieu. A geometric dilution series from 10 to 0.625 colony forming unit per 10 ml culture media was used. Subsequent to the evaluation the second part of the study started including the validation of the fertility of the culture media and the parallel testing of the two methods by investigating products. For this purpose two product batches were tested in three independent runs. Concerning the validation we could not find any aberration between the alternative and the reference method. In addition, the recovery rate of each microorganism was between 83.33 and 100 %. The alternative method showed non-inferiority regarding accuracy to the reference method. Due to this study we reduced the sterility testing for cornea and amniotic grafts to 9 days. PMID:24810914

  9. Wastes and by-products - alternatives for agricultural use

    SciTech Connect

    Boles, J.L.; Craft, D.J.; Parker, B.R.

    1994-10-01

    Top address a growing national problem with generation of wastes and by-products, TVA has been involved for several years with developing and commercializing environmentally responsible practices for eliminating, minimizing, or utilizing various wastes/by-products. In many cases, reducing waste generation is impractical, but the wastes/by-products can be converted into other environmentally sound products. In some instances, conversion of safe, value-added agricultural products in the best or only practical alternative. TVA is currently involved with a diversity of projects converting wastes/by-products into safe, economical, and agriculturally beneficial products. Environmental improvement projects have involved poultry litter, cellulosic wastes, used battery acid, ammonium sulfate fines, lead smelting effluents, deep-welled sulfuric acid/ammonium bisulfate solutions, wood ash, waste magnesium ammonium sulfate slurry from recording tape production, and ammunition plant waste sodium nitrate/ammonium nitrate streams.

  10. Real-Time Tropospheric Delay Estimation using IGS Products

    NASA Astrophysics Data System (ADS)

    Stürze, Andrea; Liu, Sha; Söhne, Wolfgang

    2014-05-01

    The Federal Agency for Cartography and Geodesy (BKG) routinely provides zenith tropospheric delay (ZTD) parameter for the assimilation in numerical weather models since more than 10 years. Up to now the results flowing into the EUREF Permanent Network (EPN) or E-GVAP (EUMETNET EIG GNSS water vapour programme) analysis are based on batch processing of GPS+GLONASS observations in differential network mode. For the recently started COST Action ES1206 about "Advanced Global Navigation Satellite Systems tropospheric products for monitoring severe weather events and climate" (GNSS4SWEC), however, rapid updates in the analysis of the atmospheric state for nowcasting applications require changing the processing strategy towards real-time. In the RTCM SC104 (Radio Technical Commission for Maritime Services, Special Committee 104) a format combining the advantages of Precise Point Positioning (PPP) and Real-Time Kinematic (RTK) is under development. The so-called State Space Representation approach is defining corrections, which will be transferred in real-time to the user e.g. via NTRIP (Network Transport of RTCM via Internet Protocol). Meanwhile messages for precise orbits, satellite clocks and code biases compatible to the basic PPP mode using IGS products are defined. Consequently, the IGS Real-Time Service (RTS) was launched in 2013 in order to extend the well-known precise orbit and clock products by a real-time component. Further messages e.g. with respect to ionosphere or phase biases are foreseen. Depending on the level of refinement, so different accuracies up to the RTK level shall be reachable. In co-operation of BKG and the Technical University of Darmstadt the real-time software GEMon (GREF EUREF Monitoring) is under development. GEMon is able to process GPS and GLONASS observation and RTS product data streams in PPP mode. Furthermore, several state-of-the-art troposphere models, for example based on numerical weather prediction data, are implemented. Hence, it

  11. Methyl Bromide Alternatives for Floriculture Production in a Problem Site

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Successful methyl bromide alternatives must manage a variety of pest problems in floriculture and vegetable production systems including weeds, plant-parasitic nematodes, and soil-borne diseases. Methods that may be successful in some situations may be challenged in sites with unusually heavy pest p...

  12. REPRODUCTIVE EFFECTS OF ALTERNATE DISINFECTANTS AND THEIR BY-PRODUCTS

    EPA Science Inventory

    This is a review article of the reproductive effects of alternate disinfectants and their by-products. The available literature is surveyed concerning studies of chlorine, chloramine, chlorine dioxide, chlorite, and chlorate. A more detailed discussion is given of reproductive st...

  13. STRAWBERRY PRODUCTION WITH ALTERNATIVE FUMIGANTS APPLIED THROUGH DRIP IRRIGATION SYSTEMS.

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Nearly all California strawberry fields are fumigated before planting. Most are fumigated with methyl bromide, which is being phased out because it has been shown to deplete stratospheric ozone. We are seeking alternative fumigants that can be used for strawberry production. This particular resea...

  14. COATING ALTERNATIVES GUIDE (CAGE) FOR METAL PARTS AND PRODUCTS PAINTING

    EPA Science Inventory

    The paper discusses the initial development of a Coating Alternatives Guide (CAGE) for metal parts and products painting. t is an innovative technology transfer approach that provides a tool to improve technology diffusion and assistance. t will provide vital, user-accessible inf...

  15. Effective Product Recommendation using the Real-Time Web

    NASA Astrophysics Data System (ADS)

    Esparza, Sandra Garcia; O'Mahony, Michael P.; Smyth, Barry

    The so-called real-time web (RTW) is a web of opinions, comments, and personal viewpoints, often expressed in the form of short, 140-character text messages providing abbreviated and highly personalized commentary in real-time. Today, Twitter is undoubtedly the king of the RTW. It boasts 190 million users and generates in the region of 65m tweets per day1. This RTW data is far from the structured data (movie ratings, product features, etc.) that is familiar to recommender systems research but it is useful to consider its applicability to recommendation scenarios. In this paper we consider harnessing the real-time opinions of users, expressed through the Twitter-like short textual reviews available on the Blippr service (www.blippr.com). In particular we describe how users and products can be represented from the terms used in their associated reviews and describe experiments to highlight the recommendation potential of this RTW data-source and approach.

  16. Botanical alternatives to antibiotics for use in organic poultry production.

    PubMed

    Diaz-Sanchez, Sandra; D'Souza, Doris; Biswas, Debrabrata; Hanning, Irene

    2015-06-01

    The development of antibiotic resistant pathogens has resulted from the use of sub-therapeutic concentrations of antibiotics delivered in poultry feed. Furthermore, there are a number of consumer concerns regarding the use of antibiotics in food animals including residue contamination of poultry products and antibiotic resistant bacterial pathogens. These issues have resulted in recommendations to reduce the use of antibiotics as growth promoters in livestock in the United States. Unlike conventional production, organic systems are not permitted to use antibiotics. Thus, both conventional and organic poultry production need alternative methods to improve growth and performance of poultry. Herbs, spices, and various other plant extracts are being evaluated as alternatives to antibiotics and some do have growth promoting effects, antimicrobial properties, and other health-related benefits. This review aims to provide an overview of herbs, spices, and plant extracts, currently defined as phytobiotics as potential feed additives. PMID:25743421

  17. Naval Petroleum Reserve No. 1: an assessment of production alternatives

    SciTech Connect

    Not Available

    1984-07-30

    Under existing legislation, every 3 years the President must decide whether to shut-in or continue production of the Naval Petroleum Reserve No. 1 (NPR-1) oil field at Elk Hills, California. The current authorization for production expires on April 5, 1985. GAO discusses the geologic, budgetary, local economic, and national security implications of three production alternatives for NPR-1: continued production, shut-in, and partial shut in. In addition, GAO discusses the advantages and disadvantages of establishing a Defense Petroleum Reserve, a crude oil reserve for the military, using part of the revenues from continued production at NPR-1 to fund it. During the course of its review, GAO found that production rates at Elk Hills may be too high, causing problems within the reserve that could decrease ultimate recovery of oil by about 139 million barrels. The Department of Energy plans to analyze this situation and, if need be, adjust the rate. 2 figures, 2 tables.

  18. Biotechnological processes for biodiesel production using alternative oils.

    PubMed

    Azócar, Laura; Ciudad, Gustavo; Heipieper, Hermann J; Navia, Rodrigo

    2010-10-01

    As biodiesel (fatty acid methyl ester (FAME)) is mainly produced from edible vegetable oils, crop soils are used for its production, increasing deforestation and producing a fuel more expensive than diesel. The use of waste lipids such as waste frying oils, waste fats, and soapstock has been proposed as low-cost alternative feedstocks. Non-edible oils such as jatropha, pongamia, and rubber seed oil are also economically attractive. In addition, microalgae, bacteria, yeast, and fungi with 20% or higher lipid content are oleaginous microorganisms known as single cell oil and have been proposed as feedstocks for FAME production. Alternative feedstocks are characterized by their elevated acid value due to the high level of free fatty acid (FFA) content, causing undesirable saponification reactions when an alkaline catalyst is used in the transesterification reaction. The production of soap consumes the conventional catalyst, diminishing FAME production yield and simultaneously preventing the effective separation of the produced FAME from the glycerin phase. These problems could be solved using biological catalysts, such as lipases or whole-cell catalysts, avoiding soap production as the FFAs are esterified to FAME. In addition, by-product glycerol can be easily recovered, and the purification of FAME is simplified using biological catalysts. PMID:20697706

  19. Alternative Blood Products and Clinical Needs in Transfusion Medicine

    PubMed Central

    Whitsett, Carolyn; Vaglio, Stefania; Grazzini, Giuliano

    2012-01-01

    The primary focus of national blood programs is the provision of a safe and adequate blood supply. This goal is dependent on regular voluntary donations and a regulatory infrastructure that establishes and enforces standards for blood safety. Progress in ex vivo expansion of blood cells from cell sources including peripheral blood, cord blood, induced pluripotent stem cells, and human embryonic stem cell lines will likely make alternative transfusion products available for clinical use in the near future. Initially, alloimmunized patients and individuals with rare blood types are most likely to benefit from alternative products. However, in developed nations voluntary blood donations are projected to be inadequate in the future as blood usage by individuals 60 years and older increases. In developing nations economic and political challenges may impede progress in attaining self-sufficiency. Under these circumstances, ex vivo generated red cells may be needed to supplement the general blood supply. PMID:22567025

  20. Lepidopteran cells, an alternative for the production of recombinant antibodies?

    PubMed Central

    Cérutti, Martine; Golay, Josée

    2012-01-01

    Monoclonal antibodies are used with great success in many different therapeutic domains. In order to satisfy the growing demand and to lower the production cost of these molecules, many alternative systems have been explored. Among them, the baculovirus/insect cells system is a good candidate. This system is very safe, given that the baculoviruses have a highly restricted host range and they are not pathogenic to vertebrates or plants. But the major asset is the speed with which it is possible to obtain very stable recombinant viruses capable of producing fully active proteins whose glycosylation pattern can be modulated to make it similar to the human one. These features could ultimately make the difference by enabling the production of antibodies with very low costs. However, efforts are still needed, in particular to increase production rates and thus make this system commercially viable for the production of these therapeutic agents. PMID:22531440

  1. Space Weather opportunity from Swarm products in near real time

    NASA Astrophysics Data System (ADS)

    Stolle, C.; Floberghagen, R.; Luhr, H.; Maus, S.; Knudsen, D. J.; Alken, P.; Doornbos, E.; Hamilton, B.; Thomson, A. W.; Visser, P. N.

    2012-12-01

    The Swarm constellation mission will provide multipoint measurements of neutral and plasma parameters describing the properties of the upper atmosphere, the ionosphere and magnetosphere and their coupling processes. Several quantities derived from Swarm observations, such as the Total Electron Content, radial and field-aligned currents, equatorial electric fields, a low-latitude plasma irregularity indicator, thermospheric density, and magnetic field signatures from magnetospheric currents, will be distributed by ESA as so-called Level-2 products. The Swarm payloads, however, provide even further opportunities for scientific investigations and services for near-Earth space exploration. This talk will present certain Swarm data products that can be offered for dedicated activities in space situational awareness and near real-time application. Examples are warning systems for critical events or assimilation models for now- and forecasting the state of the near-Earth space. Associated requirements concerning product specification and product delay will be discussed.

  2. Energy cane as a multiple-products alternative

    SciTech Connect

    Alexander, A.G.

    1984-01-01

    CANE SUGAR planting as it was formerly known is in serious and essentially irreversible trouble. Diversification of sugarcane to alternative farm crops is indicated in some instances. Yet, for the most part, the more logical alternative is an internal diversification to a multiple-products biomass commodity. Sometimes termed the energy cane approach, its keystones are the management of sugarcane as a quantitative rather than qualitative entity, and the inclusion of certain tropical-grass relatives to assist cane in its year-round supply of biomass to industrial consumers. Managed in this way, absolute tonnages of whole cane are increased materially beyond what is possible from sugar-crop management. Juice quality declines but sugar yields are significant as a function of high biomass tonnages per acre. Usage of the lignocellulose can range from low-quality humid boiler fuel in furnaces designed for refuse incineration, to higher-quality fuels in more efficient boilers, to proprietary fuels and chemical products, and to lignocellulose supply as the feedstock for primary chemicals production. The latter might include, for example, synthesis gas and petrochemicals in tropical regions lacking natural gas, naphtha, or coal as starting materials. Diversification of sugarcane to completely new farm commodities is opposed in favor of internal diversification to a high-growth, multiple-products commodity. Decisive issues here are as much educational as they are technical. The energy cane concept maintains that sugarcane is a future resource of enormous national and international value. It should develop accordingly where decision-taking is by persons who respect the cane plant and who have done their homework on its alternative-use potentials. 35 references, 5 figures, 6 tables.

  3. Antibiotics in Canadian poultry productions and anticipated alternatives.

    PubMed

    Diarra, Moussa S; Malouin, François

    2014-01-01

    The use of antibiotics in food-producing animals has significantly increased animal health by lowering mortality and the incidence of diseases. Antibiotics also have largely contributed to increase productivity of farms. However, antibiotic usage in general and relevance of non-therapeutic antibiotics (growth promoters) in feed need to be reevaluated especially because bacterial pathogens of humans and animals have developed and shared a variety of antibiotic resistance mechanisms that can easily be spread within microbial communities. In Canada, poultry production involves more than 2600 regulated chicken producers who have access to several antibiotics approved as feed additives for poultry. Feed recipes and mixtures vary greatly geographically and from one farm to another, making links between use of a specific antibiotic feed additive and production yields or selection of specific antibiotic-resistant bacteria difficult to establish. Many on-farm studies have revealed the widespread presence of antibiotic-resistant bacteria in broiler chickens. While some reports linked the presence of antibiotic-resistant organisms to the use of feed supplemented with antibiotics, no recent studies could clearly demonstrate the benefit of antimicrobial growth promoters on performance and production yields. With modern biosecurity and hygienic practices, there is a genuine concern that intensive utilization of antibiotics or use of antimicrobial growth promoters in feed might no longer be useful. Public pressure and concerns about food and environmental safety (antibiotic residues, antibiotic-resistant pathogens) have driven researchers to actively look for alternatives to antibiotics. Some of the alternatives include pre- and probiotics, organic acids and essential oils. We will describe here the properties of some bioactive molecules, like those found in cranberry, which have shown interesting polyvalent antibacterial and immuno-stimulatory activities. PMID:24987390

  4. Antibiotics in Canadian poultry productions and anticipated alternatives

    PubMed Central

    Diarra, Moussa S.; Malouin, François

    2014-01-01

    The use of antibiotics in food-producing animals has significantly increased animal health by lowering mortality and the incidence of diseases. Antibiotics also have largely contributed to increase productivity of farms. However, antibiotic usage in general and relevance of non-therapeutic antibiotics (growth promoters) in feed need to be reevaluated especially because bacterial pathogens of humans and animals have developed and shared a variety of antibiotic resistance mechanisms that can easily be spread within microbial communities. In Canada, poultry production involves more than 2600 regulated chicken producers who have access to several antibiotics approved as feed additives for poultry. Feed recipes and mixtures vary greatly geographically and from one farm to another, making links between use of a specific antibiotic feed additive and production yields or selection of specific antibiotic-resistant bacteria difficult to establish. Many on-farm studies have revealed the widespread presence of antibiotic-resistant bacteria in broiler chickens. While some reports linked the presence of antibiotic-resistant organisms to the use of feed supplemented with antibiotics, no recent studies could clearly demonstrate the benefit of antimicrobial growth promoters on performance and production yields. With modern biosecurity and hygienic practices, there is a genuine concern that intensive utilization of antibiotics or use of antimicrobial growth promoters in feed might no longer be useful. Public pressure and concerns about food and environmental safety (antibiotic residues, antibiotic-resistant pathogens) have driven researchers to actively look for alternatives to antibiotics. Some of the alternatives include pre- and probiotics, organic acids and essential oils. We will describe here the properties of some bioactive molecules, like those found in cranberry, which have shown interesting polyvalent antibacterial and immuno-stimulatory activities. PMID:24987390

  5. Functionality of alternative protein in gluten-free product development.

    PubMed

    Deora, Navneet Singh; Deswal, Aastha; Mishra, Hari Niwas

    2015-07-01

    Celiac disease is an immune-mediated disease triggered in genetically susceptible individuals by ingested gluten from wheat, rye, barley, and other closely related cereal grains. The current treatment for celiac disease is life-long adherence to a strict gluten-exclusion diet. The replacement of gluten presents a significant technological challenge, as it is an essential structure-building protein, which is necessary for formulating high-quality baked goods. A major limitation in the production of gluten-free products is the lack of protein functionality in non-wheat cereals. Additionally, commercial gluten-free mixes usually contain only carbohydrates, which may significantly limit the amount of protein in the diet. In the recent past, various approaches are attempted to incorporate protein-based ingredients and to modify the functional properties for gluten-free product development. This review aims to the highlight functionality of the alternative protein-based ingredients, which can be utilized for gluten-free product development both functionally as well as nutritionally. PMID:26048849

  6. Real Time Volcanic Cloud Products and Predictions for Aviation Alerts

    NASA Technical Reports Server (NTRS)

    Krotkov, Nickolay A.; Habib, Shahid; da Silva, Arlindo; Hughes, Eric; Yang, Kai; Brentzel, Kelvin; Seftor, Colin; Li, Jason Y.; Schneider, David; Guffanti, Marianne; Hoffman, Robert L.; Myers, Tim; Tamminen, Johanna; Hassinen, Seppo

    2014-01-01

    Volcanic eruptions can inject significant amounts of sulfur dioxide (SO2) and volcanic ash into the atmosphere, posing a substantial risk to aviation safety. Ingesting near-real time and Direct Readout satellite volcanic cloud data is vital for improving reliability of volcanic ash forecasts and mitigating the effects of volcanic eruptions on aviation and the economy. NASA volcanic products from the Ozone Monitoring Insrument (OMI) aboard the Aura satellite have been incorporated into Decision Support Systems of many operational agencies. With the Aura mission approaching its 10th anniversary, there is an urgent need to replace OMI data with those from the next generation operational NASA/NOAA Suomi National Polar Partnership (SNPP) satellite. The data provided from these instruments are being incorporated into forecasting models to provide quantitative ash forecasts for air traffic management. This study demonstrates the feasibility of the volcanic near-real time and Direct Readout data products from the new Ozone Monitoring and Profiling Suite (OMPS) ultraviolet sensor onboard SNPP for monitoring and forecasting volcanic clouds. The transition of NASA data production to our operational partners is outlined. Satellite observations are used to constrain volcanic cloud simulations and improve estimates of eruption parameters, resulting in more accurate forecasts. This is demonstrated for the 2012 eruption of Copahue. Volcanic eruptions are modeled using the Goddard Earth Observing System, Version 5 (GEOS-5) and the Goddard Chemistry Aerosol and Radiation Transport (GOCART) model. A hindcast of the disruptive eruption from Iceland's Eyjafjallajokull is used to estimate aviation re-routing costs using Metron Aviation's ATM Tools.

  7. Monolithic uncooled IR detectors of polycrystalline PbSe: a real alternative

    NASA Astrophysics Data System (ADS)

    Vergara, G.; Gómez, L. J.; Villamayor, V.; Álvarez, M.; Torquemada, M. C.; Rodrigo, M. T.; Verdú, M.; Sánchez, F. J.; Almazán, R. M.; Plaza, J.; Rodriguez, P.; Catalán, I.; Gutierrez, R.; Montojo, M. T.; Serra-Graells, F.; Margarit, J. M.; Terés, L.

    2007-04-01

    Paradoxically more than 50 years after being used in WWII, polycrystalline PbSe technology has turned today into an emerging technology. Without any doubt one of the main facts responsible for the PbSe resurgence is a new method for processing detectors based on a Vapour Phase Deposition (VPD) technique developed at CIDA. Using this method, the first low density 2D PbSe Focal Plane Array (FPA), an x-y addressed type device, was processed on silicon. Even though the last advances have been important they are not yet enough to consider this technology as a real alternative to other uncooled technologies. To reach technical relevance and commercial interest it is obligated to integrate monolithically or hybridize the sensors with their corresponding read out electronics (ROIC). Aiming to process monolithic devices, a proper CMOS read out electronics were designed. In parallel, enabled technologies were developed for adapting the material peculiarities to the CMOS substrates. In this work, the first monolithic device of VPD PbSe is presented. Even though it is a modest 16x16 FPA with a pitch of 200 μm, it represents an important milestone, allocating polycrystalline PbSe among the major players in the short list of uncooled IR detectors. Unlike microbolometers and ferroelectrics, it is a photonic detector suitable for being used as a detector in low cost IR imagers sensitive to the MWIR band and with frame rates as high as 1000 fps. The number of applications is therefore huge, some of them specific, unique and highly demanded in the military and security fields such as sensors applied to fast imagers, Active Protection Systems or low cost seekers.

  8. Alternative downstream processes for production of antibodies and antibody fragments.

    PubMed

    Arakawa, Tsutomu; Tsumoto, Kouhei; Ejima, Daisuke

    2014-11-01

    Protein-A or Protein-L affinity chromatography and virus inactivation are key processes for the manufacturing of therapeutic antibodies and antibody fragments. These two processes often involve exposure of therapeutic proteins to denaturing low pH conditions. Antibodies have been shown to undergo conformational changes at low pH, which can lead to irreversible damages on the final product. Here, we review alternative downstream approaches that can reduce the degree of low pH exposure and consequently damaged product. We and others have been developing technologies that minimize or eliminate such low pH processes. We here cover facilitated elution of antibodies using arginine in Protein-A and Protein-G affinity chromatography, a more positively charged amidated Protein-A, two Protein-A mimetics (MEP and Mabsorbent), mixed-mode and steric exclusion chromatography, and finally enhanced virus inactivation by solvents containing arginine. This article is part of a Special Issue entitled: Recent advances in molecular engineering of antibody. PMID:24859179

  9. Summary of Plutonium-238 Production Alternatives Analysis Final Report

    SciTech Connect

    James Werner; Wade E. Bickford; David B. Lord; Chadwick D. Barklay

    2013-03-01

    The Team implemented a two-phase evaluation process. During the first phase, a wide variety of past and new candidate facilities and processing methods were assessed against the criteria established by DOE for this assessment. Any system or system element selected for consideration as an alternative within the project to reestablish domestic production of Pu-238 must meet the following minimum criteria: Any required source material must be readily available in the United States, without requiring the development of reprocessing technologies or investments in systems to separate material from identified sources. It must be cost, schedule, and risk competitive with existing baseline technology. Any identified facilities required to support the concept must be available to the program for the entire project life cycle (notionally 35 years, unless the concept is so novel as to require a shorter duration). It must present a solution that can generate at least 1.5 Kg of Pu-238 oxide per year, for at least 35 years. It must present a low-risk, near-term solution to the National Aeronautics and Space Administration’s urgent mission need. DOE has implemented this requirement by eliminating from project consideration any alternative with key technologies at less than Technology Readiness Level 5. The Team evaluated the options meeting these criteria using a more detailed assessment of the reasonable facility variations and compared them to the preferred option, which consists of target irradiation at the Advanced Test Reactor (ATR) and the High Flux Isotope Reactor (HFIR), target fabrication and chemical separations processing at the ORNL Radiochemical Engineering Development Center, and neptunium 237 storage at the Materials and Fuels Complex at INL. This preferred option is consistent with the Records of Decision from the earlier National Environmental Policy Act (NEPA) documentation

  10. Advanced Electrochemical Technologies for Hydrogen Production by Alternative Thermochemical Cycles

    SciTech Connect

    Lvov, Serguei; Chung, Mike; Fedkin, Mark; Lewis, Michele; Balashov, Victor; Chalkova, Elena; Akinfiev, Nikolay; Stork, Carol; Davis, Thomas; Gadala-Maria, Francis; Stanford, Thomas; Weidner, John; Law, Victor; Prindle, John

    2011-01-06

    Hydrogen fuel is a potentially major solution to the problem of climate change, as well as addressing urban air pollution issues. But a key future challenge for hydrogen as a clean energy carrier is a sustainable, low-cost method of producing it in large capacities. Most of the world's hydrogen is currently derived from fossil fuels through some type of reforming processes. Nuclear hydrogen production is an emerging and promising alternative to the reforming processes for carbon-free hydrogen production in the future. This report presents the main results of a research program carried out by a NERI Consortium, which consisted of Penn State University (PSU) (lead), University of South Carolina (USC), Tulane University (TU), and Argonne National Laboratory (ANL). Thermochemical water decomposition is an emerging technology for large-scale production of hydrogen. Typically using two or more intermediate compounds, a sequence of chemical and physical processes split water into hydrogen and oxygen, without releasing any pollutants externally to the atmosphere. These intermediate compounds are recycled internally within a closed loop. While previous studies have identified over 200 possible thermochemical cycles, only a few have progressed beyond theoretical calculations to working experimental demonstrations that establish scientific and practical feasibility of the thermochemical processes. The Cu-Cl cycle has a significant advantage over other cycles due to lower temperature requirements – around 530 °C and below. As a result, it can be eventually linked with the Generation IV thermal power stations. Advantages of the Cu-Cl cycle over others include lower operating temperatures, ability to utilize low-grade waste heat to improve energy efficiency, and potentially lower cost materials. Another significant advantage is a relatively low voltage required for the electrochemical step (thus low electricity input). Other advantages include common chemical agents and

  11. Alternative Ways to Index Farm Real Estate Values. Technical Bulletin Number 1724.

    ERIC Educational Resources Information Center

    Greene, Catherine; Barnard, Charles

    The U.S. Department of Agriculture's (USDA) farm real estate value index is currently constructed with a modified Laspeyres weighted average formula, with the acreage weights changed approximately every 10 years. An analysis of acreage weights from 1974, 1978, and 1982 census data revealed, however, that substantial changes in real estate values…

  12. Use of MRF residue as alternative fuel in cement production.

    PubMed

    Fyffe, John R; Breckel, Alex C; Townsend, Aaron K; Webber, Michael E

    2016-01-01

    Single-stream recycling has helped divert millions of metric tons of waste from landfills in the U.S., where recycling rates for municipal solid waste are currently over 30%. However, material recovery facilities (MRFs) that sort the municipal recycled streams do not recover 100% of the incoming material. Consequently, they landfill between 5% and 15% of total processed material as residue. This residue is primarily composed of high-energy-content non-recycled plastics and fiber. One possible end-of-life solution for these energy-dense materials is to process the residue into Solid Recovered Fuel (SRF) that can be used as an alternative energy resource capable of replacing or supplementing fuel resources such as coal, natural gas, petroleum coke, or biomass in many industrial and power production processes. This report addresses the energetic and environmental benefits and trade-offs of converting non-recycled post-consumer plastics and fiber derived from MRF residue streams into SRF for use in a cement kiln. An experimental test burn of 118 Mg of SRF in the precalciner portion of the cement kiln was conducted. The SRF was a blend of 60% MRF residue and 40% post-industrial waste products producing an estimated 60% plastic and 40% fibrous material mixture. The SRF was fed into the kiln at 0.9 Mg/h for 24h and then 1.8 Mg/h for the following 48 h. The emissions data recorded in the experimental test burn were used to perform the life-cycle analysis portion of this study. The analysis included the following steps: transportation, landfill, processing and fuel combustion at the cement kiln. The energy use and emissions at each step is tracked for the two cases: (1) The Reference Case, where MRF residue is disposed of in a landfill and the cement kiln uses coal as its fuel source, and (2) The SRF Case, in which MRF residue is processed into SRF and used to offset some portion of coal use at the cement kiln. The experimental test burn and accompanying analysis indicate

  13. MSWT-01, an alternative in combining Production Based Education (PBE) and student CSR program in Polman

    NASA Astrophysics Data System (ADS)

    Ananto, Gamawan; Setiawan, Albertus B.; Z, Darman M.

    2014-06-01

    MSWT-01, Mobile Surface Water Treatment, producing 1m3 per hour, is an alternative for providing clean water in flood disaster areas, and was developed at Bandung State Polytechnic for Manufacturing (Polman) as a part of institution research project. The combination of cartridge or membrane technology such as carbon block, MF, UF and filtration media is used for this machine, instead of coagulation-flocculation with chemical addition, due to emergency purposes related with its treatment processing time. The idea is that MSWT could be combined with Production Based Education (PBE) concept in Polman as a vocational education institution and students 'CSR', students social activities. With the number of implementation trials in real flood area condition, MSWT will be developed further based on the technical output result. The manufacturing process for improving or adding necessary features could be implemented as a student's project in PBE system. This might be an ideal combination alternative for such vocational institution that students get the product media for their PBE program and implement their work as a defined social activity. They will learn and experience related technical matters and more social interactions with the people and other disaster stakeholder as well.

  14. Production cost of a real microalgae production plant and strategies to reduce it.

    PubMed

    Acién, F G; Fernández, J M; Magán, J J; Molina, E

    2012-01-01

    The cost analysis of a real facility for the production of high value microalgae biomass is presented. The facility is based on ten 3 m3 tubular photobioreactors operated in continuous mode for 2 years, data of Scenedesmus almeriensis productivity but also of nutrients and power consumption from this facility being used. The yield of the facility was close to maximum expected for the location of Almería, the annual production capacity being 3.8 t/year (90 t/ha·year) and the photosynthetic efficiency being 3.6%. The production cost was 69 €/kg. Economic analysis shows that labor and depreciation are the major factors contributing to this cost. Simplification of the technology and scale-up to a production capacity of 200 t/year allows to reduce the production cost up to 12.6 €/kg. Moreover, to reduce the microalgae production cost to approaches the energy or commodities markets it is necessary to reduce the photobioreactor cost (by simplifying its design or materials used), use waste water and flue gases, and reduce the power consumption and labor required for the production step. It can be concluded that although it has been reported that production of biofuels from microalgae is relatively close to being economically feasible, data here reported demonstrated that to achieve it by using the current production technologies, it is necessary to substantially reduce their costs and to operate them near their optimum values. PMID:22361647

  15. Biodiesel From Alternative Oilseed Feedstocks: Production and Properties

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Fatty acid methyl esters were prepared and evaluated as potential biodiesel fuels from several alternative oilseed feedstocks, which included camelina (Camelina sativa L.), coriander (Coriandrum sativum L.), field mustard (Brassica juncea L.), field pennycress (Thlaspi arvense L.), and meadowfoam (L...

  16. Use of 'natural' products as alternatives to antibiotic feed additives in ruminant production.

    PubMed

    Jouany, J-P; Morgavi, D P

    2007-11-01

    The banning in 2006 of the use of antibiotics as animal growth promoters in the European Union has increased demand from producers for alternative feed additives that can be used to improve animal production. This review gives an overview of the most common non-antibiotic feed additives already being used or that could potentially be used in ruminant nutrition. Probiotics, dicarboxylic acids, enzymes and plant-derived products including saponins, tannins and essential oils are presented. The known modes of action and effects of these additives on feed digestion and more especially on rumen fermentations are described. Their utility and limitations in field conditions for modern ruminant production systems and their compliance with the current legislation are also discussed. PMID:22444918

  17. Problem Solving in Real-Life Contexts: An Alternative for Design in Technology Education.

    ERIC Educational Resources Information Center

    Hill, Ann Marie

    1998-01-01

    Examines technology education through technological problem solving in real-life contexts. Discusses theoretical framework and presents case studies from Ontario (Canada) elementary and secondary schools: writing and producing a play with grade 1 students, and community projects with grade 10 and 11 manufacturing technology students. Outlines…

  18. Banana production systems: identification of alternative systems for more sustainable production.

    PubMed

    Bellamy, Angelina Sanderson

    2013-04-01

    Large-scale, monoculture production systems dependent on synthetic fertilizers and pesticides, increase yields, but are costly and have deleterious impacts on human health and the environment. This research investigates variations in banana production practices in Costa Rica, to identify alternative systems that combine high productivity and profitability, with reduced reliance on agrochemicals. Farm workers were observed during daily production activities; 39 banana producers and 8 extension workers/researchers were interviewed; and a review of field experiments conducted by the National Banana Corporation between 1997 and 2002 was made. Correspondence analysis showed that there is no structured variation in large-scale banana producers' practices, but two other banana production systems were identified: a small-scale organic system and a small-scale conventional coffee-banana intercropped system. Field-scale research may reveal ways that these practices can be scaled up to achieve a productive and profitable system producing high-quality export bananas with fewer or no pesticides. PMID:23055273

  19. Alternative substrates in production of trees in 25-gallon containers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Pine bark supplies have wavered in availability over the past couple of years due to a shift in-field harvesting where bark is used as a biofuel material. Research in alternative potting substrates has continued across the country in an attempt to identify inexpensive, and logistically available, s...

  20. Calla lily production with methyl bromide alternatives – Year 2

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Cut flower and ornamental bulb industries rely heavily on a methyl bromide/chloropicrin (MB/Pic) mixture as a key pest management tool. The loss of MB will seriously affect the cut flower and bulb industry, and, in the future, will require growers to use alternative fumigants. Past experiments have...

  1. Optimizing Alternative Fumigant Applications for Ornamental Production in Florida

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In Florida, cut flower and other ornamental crop producers have a very limited number of alternatives to methyl bromide for several reasons including the lack of registered herbicides available for these crops, and the need to control previously planted cultivars volunteering as weeds within the sam...

  2. A NOVEL OLEAGINOUS YEAST STRAIN WITH HIGH LIPID PRODUCTIVITY AND ITS APPLICATION TO ALTERNATIVE BIODIESEL PRODUCTION.

    PubMed

    Areesirisuk, A; Chiu, C H; Yen, T B; Liu, C H; Guo, J H

    2015-01-01

    Five lipid-producing yeast strains, CHC08, CHC11, CHC28, CHC34, and CHC35, were revealed by Sudan Black B staining to contain lipid droplets within cells. Molecular analysis demonstrated that they were 2 strains of Candida parapsilosis, Pseudozyma parantarctica, Pichia manshurica, and Pichia occidentalis. Following batch fermentation, P. parantarctica CHC28 was found to have the highest biomass concentration, total lipids and lipid content levels. The major fatty acids in the lipids of this yeast strain were C16 and C18. Predictions of the properties of yeast biodiesel using linear equations resulted in values similar to biodiesel made from plant oils. Preliminary production of yeast biodiesel from P. parantarctica CHC28 was accomplished through esterification and transesterification reactions. It was found that yeast lipids with high acid value are easily converted to biodiesel at an approximately 90% yield. Therefore, it is possible to use crude lipids as alternative raw materials for biodiesel production. PMID:26353403

  3. Determination of alternative fuels combustion products: Phase 1 report

    SciTech Connect

    Whitney, K.A.

    1997-09-01

    This report describes the laboratory effort to identify and quantify organic exhaust species generated from alternative-fueled light-duty vehicles operating over the Federal Test Procedure on compressed natural gas, liquefied petroleum gas, methanol, ethanol, and reformulated gasoline. The exhaust species from these vehicles were identified and quantified for fuel/air equivalence ratios of 0.8, 1.0, and 1.2, nominally, and were analyzed with and without a vehicle catalyst in place to determine the influence of a catalytic converter on species formation.

  4. PERFORMANCE DEMONSTRATIONS OF ALTERNATIVE SCREEN RECLAMATION PRODUCTS FOR SCREEN PRINTING

    EPA Science Inventory

    This project evaluated environmentally-preferable products for the screen reclamation process In screen printing during month-long demonstrations at 23 printing facilities nationwide. hrough the Environmental Protection Agency (EPA) Design for the Environment Printing Project, pr...

  5. AIR POLLUTION CONTROL ALTERNATIVES FOR SHALE OIL PRODUCTION OPERATIONS

    EPA Science Inventory

    The report consolidates, evaluates, and presents available air pollution emission data and air pollution control technology relevant to oil shale production, for use by project developers in preparing environmental impact statements and permit applications under Clean Air Act and...

  6. Global Near Real-Time Satellite-based Flood Monitoring and Product Dissemination

    NASA Astrophysics Data System (ADS)

    Smith, M.; Slayback, D. A.; Policelli, F.; Brakenridge, G. R.; Tokay, M.

    2012-12-01

    Flooding is among the most destructive, frequent, and costly natural disasters faced by modern society, with several major events occurring each year. In the past few years, major floods have devastated parts of China, Thailand, Pakistan, Australia, and the Philippines, among others. The toll of these events, in financial costs, displacement of individuals, and deaths, is substantial and continues to rise as climate change generates more extreme weather events. When these events do occur, the disaster management community requires frequently updated and easily accessible information to better understand the extent of flooding and better coordinate response efforts. With funding from NASA's Applied Sciences program, we have developed, and are now operating, a near real-time global flood mapping system to help provide critical flood extent information within 24-48 hours after flooding events. The system applies a water detection algorithm to MODIS imagery received from the LANCE (Land Atmosphere Near real-time Capability for EOS) system at NASA Goddard. The LANCE system typically processes imagery in less than 3 hours after satellite overpass, and our flood mapping system can output flood products within ½ hour of acquiring the LANCE products. Using imagery from both the Terra (10:30 AM local time overpass) and Aqua (1:30 PM) platforms allows an initial assessment of flooding extent by late afternoon, every day, and more robust assessments after accumulating imagery over a longer period; the MODIS sensors are optical, so cloud cover remains an issue, which is partly overcome by using multiple looks over one or more days. Other issues include the relatively coarse scale of the MODIS imagery (250 meters), the difficulty of detecting flood waters in areas with continuous canopy cover, confusion of shadow (cloud or terrain) with water, and accurately identifying detected water as flood as opposed to normal water extents. We have made progress on some of these issues

  7. Sociocultural attitudes surrounding menstruation and alternative menstrual products: the explanatory role of self-objectification.

    PubMed

    Grose, Rose Grace; Grabe, Shelly

    2014-01-01

    We extend objectification theory research to consider the relationship between self-objectification and attitudes toward an alternative menstrual product in a diverse sample of female undergraduates from the United States (N = 151). We use a survey design to investigate attitudes toward one's menstruation as a potential mechanism that may explain this relationship. Reactions to an alternative menstrual product were predominantly negative, supporting prior research on stigma and shame surrounding menstruation. Exploratory structural equation modeling revealed attitudes toward one's menstruation mediated the relationship between self-objectification and participants' reactions to an alternative menstrual product. Implications for women's health are discussed. PMID:24527840

  8. The Nuclear Alternative: Energy Production within Ulaanbaatar, Mongolia

    NASA Astrophysics Data System (ADS)

    Liodakis, Emmanouel Georgiou

    2011-06-01

    Over ninety percent of Mongolia's energy load is run through the Central Energy System. This primary grid provides Mongolia's capital, Ulaanbaatar, with the power it uses to function. In the first half of 2010 the Central Energy System managed 1739.45 million kWhs, a 4.6 percent increase from 2009. If this growth rate continues, by 2015 Ulaanbaatar's three power plants will be unable to generate enough heat and electricity to meet the city's needs. Currently, plans have been proposed to rehabilitate the aging coal power plants. However, rising maintenance costs and growing emission levels make the long-term sustainability of this solution uncertain. The following paper analyzes the capital, maintenance, and decommissioning costs associated with the current rehabilitation plans and compares them with a nuclear alternative.

  9. The Nuclear Alternative: Energy Production within Ulaanbaatar, Mongolia

    SciTech Connect

    Liodakis, Emmanouel Georgiou

    2011-06-28

    Over ninety percent of Mongolia's energy load is run through the Central Energy System. This primary grid provides Mongolia's capital, Ulaanbaatar, with the power it uses to function. In the first half of 2010 the Central Energy System managed 1739.45 million kWhs, a 4.6 percent increase from 2009. If this growth rate continues, by 2015 Ulaanbaatar's three power plants will be unable to generate enough heat and electricity to meet the city's needs. Currently, plans have been proposed to rehabilitate the aging coal power plants. However, rising maintenance costs and growing emission levels make the long-term sustainability of this solution uncertain. The following paper analyzes the capital, maintenance, and decommissioning costs associated with the current rehabilitation plans and compares them with a nuclear alternative.

  10. Komagataeibacter rhaeticus as an alternative bacteria for cellulose production.

    PubMed

    Machado, Rachel T A; Gutierrez, Junkal; Tercjak, Agnieszka; Trovatti, Eliane; Uahib, Fernanda G M; Moreno, Gabriela de Padua; Nascimento, Andresa P; Berreta, Andresa A; Ribeiro, Sidney J L; Barud, Hernane S

    2016-11-01

    A strain isolated from Kombucha tea was isolated and used as an alternative bacterium for the biosynthesis of bacterial cellulose (BC). In this study, BC generated by this novel bacterium was compared to Gluconacetobacter xylinus biosynthesized BC. Kinetic studies reveal that Komagataeibacter rhaeticus was a viable bacterium to produce BC according to yield, thickness and water holding capacity data. Physicochemical properties of BC membranes were investigated by UV-vis and Fourier transform infrared spectroscopies (FTIR), thermogravimetrical analysis (TGA) and X-ray diffraction (XRD). Additionally, scanning electron microscopy (SEM) and atomic force microscopy (AFM) were also used for morphological characterization. Mechanical properties at nano and macroscale were studied employing PeakForce quantitative nanomechanical property mapping (QNM) and dynamic mechanical analyzer (DMA), respectively. Results confirmed that BC membrane biosynthesized by Komagataeibacter rhaeticus had similar physicochemical, morphological and mechanical properties than BC membrane produced by Gluconacetobacter xylinus and can be widely used for the same applications. PMID:27516336

  11. The production of lipids alternately labelled with carbon-13.

    PubMed

    Boyle-Roden, Elizabeth; German, J B; Wood, B J B

    2003-07-01

    Chlorella cells were shown to have similar fatty acid profiles when grown photoautotrophically or if grown photoheterotrophically with ethanoate (acetate) as carbon source. When supplied with ethanoate labelled with carbon-13 in the methyl group, the alga incorporated it into fatty acids with retention of the sequence of labelling on alternate carbon atoms, thus providing a convenient method for synthesising lipids in a form useful for nuclear magnetic resonance (NMR) studies of lipids in situ in membranes. Marine algae used in fish farming may have higher levels of very highly unsaturated fatty acids; proposals for producing these compounds labelled with carbon-13 are, therefore, presented, based on using centrally labelled glycerol. The scope for producing other substances labelled in a form suitable for NMR studies, such as carotenoids, is discussed. PMID:12919810

  12. High pressure as an alternative processing step for ham production.

    PubMed

    Pingen, Sylvia; Sudhaus, Nadine; Becker, André; Krischek, Carsten; Klein, Günter

    2016-08-01

    As high pressure processing (HPP) is becoming more and more important in the food industry, this study examined the application of HPP (500 and 600MPa) as a manufacturing step during simulated ham production. By replacing conventional heating with HPP steps, ham-like texture or color attributes could not be achieved. HPP products showed a less pale, less red appearance, softer texture and higher yields. However, a combination of mild temperature (53°C) and 500MPa resulted in parameters more comparable to cooked ham. We conclude that HPP can be used for novel food development, providing novel textures and colors. However, when it comes to ham production, a heating step seems to be unavoidable to obtain characteristic ham properties. PMID:27019422

  13. Real-time sweat analysis via alternating current conductivity of artificial and human sweat

    NASA Astrophysics Data System (ADS)

    Liu, Gengchen; Alomari, Mahmoud; Sahin, Bunyamin; Snelgrove, Samuel E.; Edwards, Jeffrey; Mellinger, Axel; Kaya, Tolga

    2015-03-01

    Dehydration is one of the most profound physiological challenges that significantly affects athletes and soldiers if not detected early. Recently, a few groups have focused on dehydration detection using sweat as the main biomarker. Although there are some proposed devices, the electrical and chemical characteristics of sweat have yet to be incorporated into the validations. In this work, we have developed a simple test setup to analyze artificial sweat that is comprised the main components of human sweat. We provide theoretical and experimental details on the electrical and chemical behavior of the artificial sweat for various concentration values within a temperature range of 5 °C to 50 °C. We have also developed an efficient sweat collecting and detection system based on 3D printing. Human studies were conducted and this particular protocol has shown that dehydration starts to take effect as early as 40 min into the physical activity if there is no fluid intake during the exercise. We believe that our device will lead to developing viable real-time sweat analysis systems.

  14. Comparison of Alternative Equivalent Circuits of Induction Motor with Real Machine Data

    NASA Astrophysics Data System (ADS)

    Bradna, J.; Bauer, J.; Fligl, S.; Hlinovsky, V.

    The algorithms based on separated control of the motor flux and torque is used in order to gain the maximum performance from the induction machine. To push the efficiency and dynamics limits of the IM to its limits mostly FOC or DTC control strategies are used. Both are based on the knowledge of the hardly measurable variable-machine flux. To obtain the information about inner machine flux models based on the machine equivalent circuit are mostly used. Therefore the accuracy of the equivalent circuits has direct influence on the accuracy of the machine control. To reduce the complexity of the mathematical model the resistances and inductances are concentrated to one component and three phase winding is assumed to be symmetrical. In order to design control strategy for the induction motor, system equations and equivalent circuit must be established at first. This paper examines and compares some of the issues of adequate machine modeling and attempts to provide a firmer basis for selection of an appropriate model and to confirm or disprove the equivalence of different approaches. The results of the IM model run up are then compared to the results obtained from the measurements on the real machine and the equivalency is discussed.

  15. Impact of Alternative Environmental Flow Prescriptions on Hydropower Production and Fish Habitat Suitability

    NASA Astrophysics Data System (ADS)

    Castellarin, A.; Ceola, S.; Pugliese, A.; Galeati, G. A.

    2015-12-01

    Anthropogenic activities along streams and rivers are increasingly recognized to be a major concern for fluvial ecosystems. The management of water resources, by means of e.g. flow diversions and dams, for industrial, agricultural, water-supply, hydropower production and flood protection purposes induces significant changes to the natural streamflow regime of a river. Indeed, the river flow regime is known to be a major abiotic factor influencing fluvial ecosystems. An established approach aimed at preserving the behaviour and distribution of fluvial species relies on the definition of minimum streamflow requirements (i.e., environmental flows) downstream of dams and diversion structures. Such environmental flows are normally identified through methodologies that have an empirical nature and may not be representative of local ecological and hydraulic conditions. While the effect of imposing a minimum discharge release is easily predictable in terms of e.g. loss of hydropower production, the advantages in terms of species preferences are often poorly understood and seldom assessed. To analyze the interactions between flow releases and the behaviour and distribution of fluvial species (i.e., from periphyton, to benthic invertebrate and fish), one may use a habitat suitability curve, which is a fundamental tool capable of describing species preferences influenced by any generic environmental variable. The outcomes of a real case study applied to several Italian rivers, located in the Marche administrative district in Central Italy (∽10000km2), in which we quantitatively assess the effects of alternative environmental flow scenarios on the existing hydropower network and on two fish species that are quite abundant in the study area (i.e., Leuciscus cephalus cabeda and Barbus barbus plebejus), will be presented and discussed. The proposed analysis, which can be easily adapted to different riparian habitats and hydrological contexts, is a useful tool to guide the

  16. Impact of alternative environmental flow prescriptions on hydropower production and fish habitat suitability

    NASA Astrophysics Data System (ADS)

    Ceola, Serena; Pugliese, Alessio; Castellarin, Attilio; Galeati, Giorgio

    2015-04-01

    Anthropogenic activities along streams and rivers are increasingly recognised to be a major concern for fluvial ecosystems. The management of water resources, by means of e.g. flow diversions and dams, for industrial, agricultural, water-supply, hydropower production and flood protection purposes induces significant changes to the natural streamflow regime of a river. Indeed, the river flow regime is known to be a major abiotic factor influencing fluvial ecosystems. An established approach aimed at preserving the behaviour and distribution of fluvial species relies on the definition of minimum streamflow requirements (i.e., environmental flows) downstream of dams and diversion structures. Such environmental flows are normally identified through methodologies that have an empirical nature and may not be representative of local ecological and hydraulic conditions. While the effect of imposing a minimum discharge release is easily predictable in terms of e.g. loss of hydropower production, the advantages in terms of species preferences are often poorly understood and seldom assessed. To analyse the interactions between flow releases and the behaviour and distribution of fluvial species (i.e., from periphyton, to benthic invertebrate and fish), one may use a habitat suitability curve, which is a fundamental tool capable of describing species preferences influenced by any generic environmental variable. The outcomes of a real case study applied to several Italian rivers, located in the Marche administrative district in Central Italy (∽10000km2), in which we quantitatively assess the effects of alternative environmental flow scenarios on the existing hydropower network and on two fish species that are quite abundant in the study area (i.e., Leuciscus cephalus cabeda and Barbus barbus plebejus), will be presented and discussed. The proposed analysis, which can be easily adapted to different riparian habitats and hydrological contexts, is a useful tool to guide the

  17. Creating Shared Instructional Products: An Alternative Approach to Improving Teaching

    ERIC Educational Resources Information Center

    Morris, Anne K.; Hiebert, James

    2011-01-01

    To solve two enduring problems in education--unacceptably large variation in learning opportunities for students across classrooms and little continuing improvement in the quality of instruction--the authors propose a system that centers on the creation of shared instructional products that guide classroom teaching. By examining systems outside…

  18. 7 CFR 58.149 - Alternate quality control programs for dairy products.

    Code of Federal Regulations, 2010 CFR

    2010-01-01

    ... 7 Agriculture 3 2010-01-01 2010-01-01 false Alternate quality control programs for dairy products... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection... for dairy products. (a) When a plant has in operation an acceptable quality control program which...

  19. 7 CFR 58.149 - Alternate quality control programs for dairy products.

    Code of Federal Regulations, 2014 CFR

    2014-01-01

    ... 7 Agriculture 3 2014-01-01 2014-01-01 false Alternate quality control programs for dairy products... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection... for dairy products. (a) When a plant has in operation an acceptable quality control program which...

  20. 7 CFR 58.149 - Alternate quality control programs for dairy products.

    Code of Federal Regulations, 2013 CFR

    2013-01-01

    ... 7 Agriculture 3 2013-01-01 2013-01-01 false Alternate quality control programs for dairy products... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection... for dairy products. (a) When a plant has in operation an acceptable quality control program which...

  1. 7 CFR 58.149 - Alternate quality control programs for dairy products.

    Code of Federal Regulations, 2012 CFR

    2012-01-01

    ... 7 Agriculture 3 2012-01-01 2012-01-01 false Alternate quality control programs for dairy products... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection... for dairy products. (a) When a plant has in operation an acceptable quality control program which...

  2. 7 CFR 58.149 - Alternate quality control programs for dairy products.

    Code of Federal Regulations, 2011 CFR

    2011-01-01

    ... 7 Agriculture 3 2011-01-01 2011-01-01 false Alternate quality control programs for dairy products... FOR GRADES OF DAIRY PRODUCTS 1 General Specifications for Dairy Plants Approved for USDA Inspection... for dairy products. (a) When a plant has in operation an acceptable quality control program which...

  3. An Alternative Framework to Evaluate Proof Productions: A Reply to Alcock and Inglis

    ERIC Educational Resources Information Center

    Weber, Keith; Mejia-Ramos, Juan Pablo

    2009-01-01

    In a recent paper, Alcock and Inglis (in press) noted a distinction between the way that Weber (in press) and they defined syntactic and semantic proof productions. Weber argued that "a syntactic proof production occurs when one works predominantly within the representation system of proof [...] Alternatively, a semantic proof production occurs…

  4. Global, Daily, Near Real-Time Satellite-based Flood Monitoring and Product Dissemination

    NASA Astrophysics Data System (ADS)

    Slayback, D. A.; Policelli, F. S.; Brakenridge, G. R.; Tokay, M. M.; Smith, M. M.; Kettner, A. J.

    2013-12-01

    Flooding is the most destructive, frequent, and costly natural disaster faced by modern society, and is expected to increase in frequency and damage with climate change and population growth. Some of 2013's major floods have impacted the New York City region, the Midwest, Alberta, Australia, various parts of China, Thailand, Pakistan, and central Europe. The toll of these events, in financial costs, displacement of individuals, and deaths, is substantial and continues to rise as climate change generates more extreme weather events. When these events do occur, the disaster management community requires frequently updated and easily accessible information to better understand the extent of flooding and better coordinate response efforts. With funding from NASA's Applied Sciences program, we developed and are now operating a near real-time global flood mapping system to help provide critical flood extent information within 24-48 hours of events. The system applies a water detection algorithm to MODIS imagery received from the LANCE (Land Atmosphere Near real-time Capability for EOS) system at NASA Goddard within a few hours of satellite overpass. Using imagery from both the Terra (10:30 AM local time overpass) and Aqua (1:30 PM) platforms allows an initial daily assessment of flooding extent by late afternoon, and more robust assessments after accumulating cloud-free imagery over several days. Cloud cover is the primary limitation in detecting surface water from MODIS imagery. Other issues include the relatively coarse scale of the MODIS imagery (250 meters), the difficulty of detecting flood waters in areas with continuous canopy cover, confusion of shadow (cloud or terrain) with water, and accurately identifying detected water as flood as opposed to normal water extents. We have made progress on many of these issues, and are working to develop higher resolution flood detection using alternate sensors, including Landsat and various radar sensors. Although these

  5. Optimizing convergence rates of alternating minimization reconstruction algorithms for real-time explosive detection applications

    NASA Astrophysics Data System (ADS)

    Bosch, Carl; Degirmenci, Soysal; Barlow, Jason; Mesika, Assaf; Politte, David G.; O'Sullivan, Joseph A.

    2016-05-01

    X-ray computed tomography reconstruction for medical, security and industrial applications has evolved through 40 years of experience with rotating gantry scanners using analytic reconstruction techniques such as filtered back projection (FBP). In parallel, research into statistical iterative reconstruction algorithms has evolved to apply to sparse view scanners in nuclear medicine, low data rate scanners in Positron Emission Tomography (PET) [5, 7, 10] and more recently to reduce exposure to ionizing radiation in conventional X-ray CT scanners. Multiple approaches to statistical iterative reconstruction have been developed based primarily on variations of expectation maximization (EM) algorithms. The primary benefit of EM algorithms is the guarantee of convergence that is maintained when iterative corrections are made within the limits of convergent algorithms. The primary disadvantage, however is that strict adherence to correction limits of convergent algorithms extends the number of iterations and ultimate timeline to complete a 3D volumetric reconstruction. Researchers have studied methods to accelerate convergence through more aggressive corrections [1], ordered subsets [1, 3, 4, 9] and spatially variant image updates. In this paper we describe the development of an AM reconstruction algorithm with accelerated convergence for use in a real-time explosive detection application for aviation security. By judiciously applying multiple acceleration techniques and advanced GPU processing architectures, we are able to perform 3D reconstruction of scanned passenger baggage at a rate of 75 slices per second. Analysis of the results on stream of commerce passenger bags demonstrates accelerated convergence by factors of 8 to 15, when comparing images from accelerated and strictly convergent algorithms.

  6. Final environmental assessment: Los Reales 115 kV transmission line alternative routes

    SciTech Connect

    Not Available

    1989-10-01

    The Central Arizona Project (CAP) was authorized as a part of the Colorado River Basin Project Act (Public Law 90-537) on September 30, 1968. The primary purpose of the CAP is to furnish water for agricultural, municipal, and industrial uses in central and southern Arizona, and western New Mexico. Due to its magnitude, the CAP is divided into several major features serving separate but interrelated functions. The Tucson Aqueduct Phase B pumping plants were designed and constructed to operate on a looped power system. The entire looped power system, including two switching stations and connecting 115-kv transmission lines, was identified in the FEIS and approved for construction in the Secretary of Interior's Record of Decision dated September 24, 1985. The loop begins in the vicinity of the Twin Peaks Pumping Plant -- the northernmost Phase B pumping station, at the Rattlesnake Switching Station. All of the looped power system has been constructed with the exception of the switching station and portion of transmission line proposed to be constructed in this project. Without construction of this final portion of the looped power system, the Phase B pumping plants will not be able to operate normally without negatively affecting nearby power sources. The CAP will also not be able to provide the reliability necessary for municipal water systems dependent upon CAP water. The purpose of this EA is to describe impacts that would result from relocating the Los Reales 115-kV transmission line, and possibly the switching station, originally identified in the FEIS. It should be mentioned the Department of Energy will complete a separate NEPA review.

  7. DISINFECTION BY-PRODUCT FORMATION BY ALTERNATIVE DISINFECTANTS AND REMOVAL BY GRANULAR ACTIVATED CARBON

    EPA Science Inventory

    The effects of the use of the alternative disinfectants on the formation of halogenated disinfection by–products (DBPs) including total organic halide, trihalomethanes, haloacetic acids, haloacetonitriles, haloketones, chloral hydrate, and chloropicrin, were examined along ...

  8. Analysis of Alternative Extensions of the Existing Production Tax Credit for Wind Generators

    EIA Publications

    2007-01-01

    Requestor: Ms. Janice Mays, Chief Counsel, Committee on Ways & Means, U.S. House of Representatives This is a letter response requesting analysis of alternative extensions of the existing production tax credit (PTC) that would apply to wind generators only.

  9. DISINFECTION BY-PRODUCT FORMATION BY ALTERNATIVE DISINFECTANTS AND REMOVAL BY GRANULAR ACTIVATED CARBON

    EPA Science Inventory

    The effects of the use of the alternative disinfectants on the formation of halogenated disinfection by-products (DBPS) including total organic halide, trihalomethanes, haloacetic acids, haloacentonitriles, haloketones, chloral hydrate, and chloropicrin, were examined along with ...

  10. NEW DISINFECTION BY-PRODUCT ISSUES: EMERGING DBP'S AND ALTERNATIVE ROUTES OF EXPOSURE

    EPA Science Inventory

    This paper discusses current issues with drinking water disinfection by-products (DBPs), which include emerging (unregulated) DBPs that can be formed at greater levels with alternative disinfectants (as compared to chlorine) and routes of human exposure (which include inhalation ...

  11. Determination of alternative fuels combustion products: Phase 3 report

    SciTech Connect

    Whitney, K.A.

    1997-12-01

    This report describes the laboratory efforts to characterize particulate and gaseous exhaust emissions from a passenger vehicle operating on alternative fuels. Tests were conducted at room temperature (nominally 72 F) and 20 F utilizing the chassis dynamometer portion of the FTP for light-duty vehicles. Fuels evaluated include Federal RFG, LPG meeting HD-5 specifications, a national average blend of CNG, E85, and M85. Exhaust particulate generated at room temperature was further characterized to determine polynuclear aromatic content, trace element content, and trace organic constituents. For all fuels except M85, the room temperature particulate emission rate from this vehicle was about 2 to 3 mg/mile. On M85, the particulate emission rate was more than 6 mg/mile. In addition, elemental analysis of particulate revealed an order of magnitude more sulfur and calcium from M85 than any other fuel. The sulfur and calcium indicate that these higher emissions might be due to engine lubricating oil in the exhaust. For RFG, particulate emissions at 20 F were more than six times higher than at room temperature. For alcohol fuels, particulate emissions at 20 F were two to three times higher than at room temperature. For CNG and LPG, particulate emissions were virtually the same at 72 F and 20 F. However, PAH emissions from CNG and LPG were higher than expected. Both gaseous fuels had larger amounts of pyrene, 1-nitropyrene, and benzo(g,h,i)perylene in their emissions than the other fuels.

  12. Products identified at an alternative disinfection pilot plant

    SciTech Connect

    Lykins, B.W. Jr.; Koffskey, W.

    1986-11-01

    Many drinking water utilities have recently changed or are seriously considering changing their disinfection practice from chlorine to some alternative treatment process. However, most of these utilities are changing their disinfectants without evaluating chemical impacts. Therefore, a research cooperative agreement was developed with Jefferson Parish, LA, to evaluate four parallel streams treated with four different disinfectants (chlorine, monochloramine, chlorine dioxide, and ozone.) These streams, along with a fifth parallel stream, which was not treated with a disinfectant (control), were passed through both sand and granular activated carbon (GAC). Ozonation reduced the total organic carbon (TOC) and total organic halide (TOX) concentration by 0.3 mg/L and 10 micrograms/L, respectively. The average concentration of TOC for the other disinfectants was comparable to that associated with the nondisinfected stream (3.3 mg/L). The average instantaneous TOX concentration for chlorine dioxide, chloramine, and chlorine disinfection after 30 min contact time increased by 60, 92, and 238 micrograms/L, respectively, from a nondisinfected concentration of 25 micrograms/L. The volatile organics most affected by disinfection (chlorination) were the trihalomethanes. No significant change in concentration was noted after disinfection for the other volatile organics evaluated, such as 1,2-dichlorethane, dichloromethane, trichloroethylene, 1,1,2-trichloroethane, and carbon tetrachloride. Ozonation produced an average concentration reduction of 11 to 84% for most of the nonvolatiles evaluated. Conversely, a concentration increase of 43 to 100% was noted, after chlorination, for some of the nonvolatile organics.

  13. Alternate Tritium Production Methods Using A Liquid Lithium Target

    SciTech Connect

    Wilson, J.

    2015-10-08

    For over 60 years, the Savannah River Site’s primary mission has been the production of tritium. From the beginning, the Savannah River National Laboratory (SRNL) has provided the technical foundation to ensure the successful execution of this critical defense mission. SRNL has developed most of the processes used in the tritium mission and provides the research and development necessary to supply this critical component. This project was executed by first developing reactor models that could be used as a neutron source. In parallel to this development calculations were carried out testing the feasibility of accelerator technologies that could also be used for tritium production. Targets were designed with internal moderating material and optimized target was calculated to be capable of 3000 grams using a 1400 MWt sodium fast reactor, 850 grams using a 400 MWt sodium fast reactor, and 100 grams using a 62 MWt reactor, annually.

  14. Development and assessment of real-time tropospheric products derived from GNSS

    NASA Astrophysics Data System (ADS)

    Vaclavovic, Pavel; Dousa, Jan; Eben, K.

    2016-07-01

    The GNSS-meteorology, i.e. monitoring the water vapour content in the troposphere through the analysis of GNSS observations, provides GNSS real-time tropospheric products in support of numerical weather prediction (NWP) and severe weather event monitoring. The real-time ultra-fast and high-resolution GNSS tropospheric products, such as zenith total delay (ZTD) and horizontal gradients describing the first-order anisotropy of the troposphere, has been developed and routinely generated at the Geodetic Observatory Pecny for several years. The poster summarizes results of the evaluation of real-time tropospheric products with respect to the IGS and EUREF final products and introduces a new online system for the monitoring and comparison of the products with other tropospheric solutions.The accuracy of real-time ZTD achieved 5-10 mm, which fulfills the requirements for NWP nowcasting applications defined by the EUMETNET EIG GNSS water vapour programme (E-GVAP). Corresponding tropospheric parameters for GNSS model were also calculated using meteorological data from numerical weather predictions and these are routinely compared with GNSS-based real-time products. The parameters from the NWP represents augmentation tropospheric corrections for a future use in applications, e.g. real-time kinematic positioning and navigation.

  15. Quartz Crystal Microbalance (QCM): An Alternative Analytical Method for Investigation in Real-Time of Liquid Properties

    NASA Astrophysics Data System (ADS)

    Cimpoca, Gh. V.; Radulescu, C.; Popescu, I. V.; Dulama, I. D.; Ionita, I.; Cimpoca, M.; Cernica, I.; Gavrila, R.

    2010-01-01

    In this paper we study the possibility to develop an alternative Analytical Method for Investigation in Real-Time of Liquid Properties, the layout and the operation with Quartz Crystal Microbalance (QCM) Systems. The quartz crystal microbalance (QCM) can be accepted as a powerful technique to monitor adsorption and desorption processes at interfaces in different chemical and biological areas. In our paper, Quartz Crystal Microbalance is used to monitor in real-time the polymer adsorption followed by azoic dye adsorption and then copolymer adsorption as well as optimization of interaction processes and determination of solution effects on the analytical signal. The solutions of azoic dye (5ṡ10-4 g/L, 5ṡ10-5 g/L and 5ṡ10-6 g/L in DMF) are adsorbed at gold electrodes of QCM and the sensor responses are estimated through decrease and increase of QCM frequency. Also, the response of the sensor at maleic anhydride (MA) copolymer with styrene St (MA-St copolymer concentration of solution: 5ṡ10-4 g/L; 5ṡ10-5 g/L and 5ṡ10-6 g/L in DMF) is fast, large, and reversible. The detailed investigation showed the fact that the Quartz Crystal Microbalance is a modern method to study a wider number of physical and chemical properties related to the surface and interfacial processes of synthesized copolymer leading to a higher reliability of the research results.

  16. The influence of base rates on correlations: An evaluation of proposed alternative effect sizes with real-world data.

    PubMed

    Babchishin, Kelly M; Helmus, Leslie-Maaike

    2016-09-01

    Correlations are the simplest and most commonly understood effect size statistic in psychology. The purpose of the current paper was to use a large sample of real-world data (109 correlations with 60,415 participants) to illustrate the base rate dependence of correlations when applied to dichotomous or ordinal data. Specifically, we examined the influence of the base rate on different effect size metrics. Correlations decreased when the dichotomous variable did not have a 50 % base rate. The higher the deviation from a 50 % base rate, the smaller the observed Pearson's point-biserial and Kendall's tau correlation coefficients. In contrast, the relationship between base rate deviations and the more commonly proposed alternatives (i.e., polychoric correlation coefficients, AUCs, Pearson/Thorndike adjusted correlations, and Cohen's d) were less remarkable, with AUCs being most robust to attenuation due to base rates. In other words, the base rate makes a marked difference in the magnitude of the correlation. As such, when using dichotomous data, the correlation may be more sensitive to base rates than is optimal for the researcher's goals. Given the magnitude of the association between the base rate and point-biserial correlations (r = -.81) and Kendall's tau (r = -.80), we recommend that AUCs, Pearson/Thorndike adjusted correlations, Cohen's d, or polychoric correlations should be considered as alternate effect size statistics in many contexts. PMID:26182856

  17. Complete solution for unambiguous discrimination of three pure states with real inner products

    SciTech Connect

    Sugimoto, H.; Hashimoto, T.; Horibe, M.; Hayashi, A.

    2010-09-15

    Complete solutions are given in a closed analytic form for unambiguous discrimination of three general pure states with real mutual inner products. For this purpose, we first establish some general results on unambiguous discrimination of n linearly independent pure states. The uniqueness of solution is proved. The condition under which the problem is reduced to an (n-1)-state problem is clarified. After giving the solution for three pure states with real mutual inner products, we examine some difficulties in extending our method to the case of complex inner products. There is a class of set of three pure states with complex inner products for which we obtain an analytical solution.

  18. Determination of alternative fuels combustion products: Phase 2 final report

    SciTech Connect

    Whitney, K.A.

    1997-06-01

    This report describes the laboratory efforts to accomplish four independent tasks: (1) speciation of hydrocarbon exhaust emissions from a light-duty vehicle operated over the chassis dynamometer portion of the light-duty FTP after modifications for operation on butane and butane blends; (2) evaluation of NREL`s Variable Conductance Vacuum Insulated Catalytic Converter Test Article 4 for the reduction of cold-start FTP exhaust emissions after extended soak periods for a Ford FFV Taurus operating on E85; (3) support of UDRI in an attempt to define correlations between engine-out combustion products identified by SwRI during chassis dynamometer testing, and those found during flow tube reactor experiments conducted by UDRI; and (4) characterization of small-diameter particulate matter from a Ford Taurus FFV operating in a simulated fuel-rich failure mode on CNG, LPG, M85, E85, and reformulated gasoline. 22 refs., 18 figs., 17 tabs.

  19. Human cell lines: A promising alternative for recombinant FIX production.

    PubMed

    de Sousa Bomfim, Aline; Cristina Corrêa de Freitas, Marcela; Picanço-Castro, Virgínia; de Abreu Soares Neto, Mário; Swiech, Kamilla; Tadeu Covas, Dimas; Maria de Sousa Russo, Elisa

    2016-05-01

    Factor IX (FIX) is a vitamin K-dependent protein, and it has become a valuable pharmaceutical in the Hemophilia B treatment. We evaluated the potential of recombinant human FIX (rhFIX) expression in 293T and SK-Hep-1 human cell lines. SK-Hep-1-FIX cells produced higher levels of biologically active protein. The growth profile of 293T-FIX cells was not influenced by lentiviral integration number into the cellular genome. SK-Hep-1-FIX cells showed a significantly lower growth rate than SK-Hep-1 cells. γ-carboxylation process is significant to FIX biological activity, thus we performed a expression analysis of genes involved in this process. The 293T gene expression suggests that this cell line could efficiently carboxylate FIX, however only 28% of the total secreted protein is active. SK-Hep-1 cells did not express high amounts of VKORC1 and carboxylase, but this cell line secreted large amounts of active protein. Enrichment of culture medium with Ca(+2) and Mg(+2) ions did not affect positively rhFIX expression in SK-Hep-1 cells. In 293T cells, the addition of 0.5 mM Ca(+2) and 1 mM Mg(+2) resulted in higher rhFIX concentration. SK-Hep-1 cell line proved to be very effective in rhFIX production, and it can be used as a novel biotechnological platform for the production of recombinant proteins. PMID:26802680

  20. Future enhancements to 3D printing and real time production

    NASA Astrophysics Data System (ADS)

    Landa, Joseph; Jenkins, Jeffery; Wu, Jerry; Szu, Harold

    2014-05-01

    The cost and scope of additive printing machines range from several hundred to hundreds of thousands of dollars. For the extra money, one can get improvements in build size, selection of material properties, resolution, and consistency. However, temperature control during build and fusing predicts outcome and protects the IP by large high cost machines. Support material options determine geometries that can be accomplished which drives cost and complexity of printing heads. Historically, 3D printers have been used for design and prototyping efforts. Recent advances and cost reduction sparked new interest in developing printed products and consumables such as NASA who is printing food, printing consumer parts (e.g. cell phone cases, novelty toys), making tools and fixtures in manufacturing, and recursively print a self-similar printer (c.f. makerbot). There is a near term promise of the capability to print on demand products at the home or office... directly from the printer to use.

  1. Nigeria`s oil production behavior: Tests of alternative hypotheses

    SciTech Connect

    Awokuse, T.O.; Jones, C.T.

    1994-12-31

    The sudden quadrupling of world oil prices in 1973-1974 marked the beginning of several formal inquiries by economists into the production behavior of members of the Organization of the Petroleum Exporting Countries (OPEC). Interest in the organization was further heightened in 1979 when nominal oil prices further doubled. However, oil market analysts have differed in their evaluation of OPEC`s role in the determination of world oil prices. Most energy economists have modeled OPEC as a cartel. Morris Adelman has suggested that OPEC`s true nature lies somewhere between two polar cases of a dominant-firm industry and an imperfect, market-sharing cartel. In the former case, one large, dominant firm (i.e., Saudi Arabia) serves as the {open_quotes}swing producer,{close_quotes} allowing other cartel members and non-OPEC oil producers to produce whatever they wished, controlling the market price by itself through its own output adjustments. The latter case of an imperfect market-sharing cartel is a loose collusive arrangement in which all members agree on an acceptable price level and individual output shares for each producer. Adelman believes that OPEC wobbles between these two cases, depending upon market conditions.

  2. ALTERNATIVE FORMULATIONS TO REDUCE CFC USE IN U.S. EXEMPTED AND EXCLUSED AEROSOL PRODUCTS

    EPA Science Inventory

    The report examines products exempted and excluded from those affected by the 1978 ban on the use of chlorofluorocarbons (CFCs) as aerosol propellants, the present consumption of CFCs still utilized for these products in the U.S., and alternative formulations which may be used to...

  3. ALTERNATIVE FORMULATIONS TO REDUCE CFC USE IN U.S. EXEMPTED AND EXCLUDED AEROSOL PRODUCTS

    EPA Science Inventory

    The report examines products exempted and excluded from those affected by the 1978 ban on the use of chlorofluorocarbons (CFCs) as aerosol propellants, the present consumption of CFCs still utilized for these products in the U.S., and alternative formulations which may be used to...

  4. Productive resources in students' ideas about energy: An alternative analysis of Watts' original interview transcripts

    NASA Astrophysics Data System (ADS)

    Harrer, Benedikt W.; Flood, Virginia J.; Wittmann, Michael C.

    2013-12-01

    For over 30 years, researchers have investigated students’ ideas about energy with the intent of reforming instructional practice. In this pursuit, Watts contributed an influential study with his 1983 paper “Some alternative views of energy” [Phys. Educ. 18, 213 (1983)]. Watts’ “alternative frameworks” continue to be used for categorizing students’ non-normative ideas about energy. Using a resources framework, we propose an alternate analysis of student responses from Watts’ interviews. In our analysis, we show how students’ activated resources about energy are disciplinarily productive. We suggest that fostering seeds of scientific understandings in students’ ideas about energy may play an important role in their development of scientific literacy.

  5. The cost of ethanol production from lignocellulosic biomass -- A comparison of selected alternative processes. Final report

    SciTech Connect

    Grethlein, H.E.; Dill, T.

    1993-04-30

    The purpose of this report is to compare the cost of selected alternative processes for the conversion of lignocellulosic biomass to ethanol. In turn, this information will be used by the ARS/USDA to guide the management of research and development programs in biomass conversion. The report will identify where the cost leverages are for the selected alternatives and what performance parameters need to be achieved to improve the economics. The process alternatives considered here are not exhaustive, but are selected on the basis of having a reasonable potential in improving the economics of producing ethanol from biomass. When other alternatives come under consideration, they should be evaluated by the same methodology used in this report to give fair comparisons of opportunities. A generic plant design is developed for an annual production of 25 million gallons of anhydrous ethanol using corn stover as the model substrate at $30/dry ton. Standard chemical engineering techniques are used to give first order estimates of the capital and operating costs. Following the format of the corn to ethanol plant, there are nine sections to the plant; feed preparation, pretreatment, hydrolysis, fermentation, distillation and dehydration, stillage evaporation, storage and denaturation, utilities, and enzyme production. There are three pretreatment alternatives considered: the AFEX process, the modified AFEX process (which is abbreviated as MAFEX), and the STAKETECH process. These all use enzymatic hydrolysis and so an enzyme production section is included in the plant. The STAKETECH is the only commercially available process among the alternative processes.

  6. Part 2 of a 4-part series Hair Products: Trends and Alternatives

    PubMed Central

    Jacob, Sharon; Katta, Rajani; Nedorost, Susan; Warshaw, Erin; Zirwas, Matt; Bhinder, Manpreet

    2011-01-01

    Objective: To provide updated data on usage of ingredients that are common potential contact allergens in several categories of hair products. To identify useful alternative products with few or no common contact allergens. Design: In November 2009, the full ingredient lists of 5,416 skin, hair, and cosmetic products marketed by the CVS pharmacy chain was copied from CVS.com into Microsoft Word format for analysis. Computer searches were made in Microsoft Word using search/replace and sorting functions to accurately identify the presence of specific allergens in each website product. Measurements: Percentages of American Contact Dermatitis Society core series allergens (and other common preservatives and sunblocks) were calculated. Results: The usage of American Contact Dermatitis Society core series allergens (and other preservatives and sunblocks) in hair products is reported. Conclusion: Data on allergens and alternatives for hair products is not widely published. This article reviews some of the common potential allergens in hair products, including shampoos, conditioners, and styling products. Suitable available alternative products for patients with contact allergy are listed. PMID:21779419

  7. NASA-Langley Web-Based Operational Real-time Cloud Retrieval Products from Geostationary Satellites

    NASA Technical Reports Server (NTRS)

    Palikonda, Rabindra; Minnis, Patrick; Spangenberg, Douglas A.; Khaiyer, Mandana M.; Nordeen, Michele L.; Ayers, Jeffrey K.; Nguyen, Louis; Yi, Yuhong; Chan, P. K.; Trepte, Qing Z.; Chang, Fu-Lung; Smith, William L, Jr.

    2006-01-01

    At NASA Langley Research Center (LaRC), radiances from multiple satellites are analyzed in near real-time to produce cloud products over many regions on the globe. These data are valuable for many applications such as diagnosing aircraft icing conditions and model validation and assimilation. This paper presents an overview of the multiple products available, summarizes the content of the online database, and details web-based satellite browsers and tools to access satellite imagery and products.

  8. Subtask 2.6 - Assessment of Alternative Fuels on CO2 Production

    SciTech Connect

    Debra Pflughoeft-Hassett; Darren Naasz

    2009-06-16

    Many coal-based electric generating units use alternative fuels, and this effort assessed the impact of alternative fuels on CO{sub 2} production and other emissions and also assessed the potential impact of changes in emission regulations under the Clean Air Act (CAA) for facilities utilizing alternative fuels that may be categorized as wastes. Information was assembled from publicly available U.S. Department of Energy Energy Information Administration databases that included alternative fuel use for 2004 and 2005. Alternative fuel types were categorized along with information on usage by coal-based electric, number of facilities utilizing each fuel type, and the heating value of solid, liquid, and gaseous alternative fuels. The sulfur dioxide, nitrogen oxide, and carbon dioxide emissions associated with alternative fuels and primary fuels were also evaluated. Carbon dioxide emissions are also associated with the transport of all fuels. A calculation of carbon dioxide emissions associated with the transport of biomass-based fuels that are typically accessed on a regional basis was made. A review of CAA emission regulations for coal-based electric generating facilities from Section 112 (1) and Section 129 (2) for solid waste incinerators was performed with consideration for a potential regulatory change from Section 112 (1) regulation to Section 129 (2). Increased emission controls would be expected to be required if coal-based electric generating facilities using alternative fuels would be recategorized under CAA Section 129 (2) for solid waste incinerators, and if this change were made, it is anticipated that coal-fired electric generating facilities might reduce the use of alternative fuels. Conclusions included information on the use profile for alternative fuels and the impacts to emissions as well as the impact of potential application of emission regulations for solid waste incinerators to electric generating facilities using alternative fuels.

  9. An evaluation of alternate production methods for Pu-238 general purpose heat source pellets

    SciTech Connect

    Mark Borland; Steve Frank

    2009-06-01

    For the past half century, the National Aeronautics and Space Administration (NASA) has used Radioisotope Thermoelectric Generators (RTG) to power deep space satellites. Fabricating heat sources for RTGs, specifically General Purpose Heat Sources (GPHSs), has remained essentially unchanged since their development in the 1970s. Meanwhile, 30 years of technological advancements have been made in the applicable fields of chemistry, manufacturing and control systems. This paper evaluates alternative processes that could be used to produce Pu 238 fueled heat sources. Specifically, this paper discusses the production of the plutonium-oxide granules, which are the input stream to the ceramic pressing and sintering processes. Alternate chemical processes are compared to current methods to determine if alternative fabrication processes could reduce the hazards, especially the production of respirable fines, while producing an equivalent GPHS product.

  10. Real Choices in Indian Resource Development: Alternatives To Leasing. AIO Report-Billings Conference (Billings, Montana, January 30, 31, and February 1, 1975).

    ERIC Educational Resources Information Center

    Americans for Indian Opportunity, Inc., Albuquerque, NM.

    Identifying the purpose of the Conference on Real Choices in Indian Resource Development: Alternatives to Leasing (Billings, Montana, January 1975) as exploring options available to American Indian tribes in the Great Plains re: the development of their own natural resources, this paper presents excerpts from participant speeches and comments…

  11. Real-time product attribute control to manufacture antibodies with defined N-linked glycan levels.

    PubMed

    Zupke, Craig; Brady, Lowell J; Slade, Peter G; Clark, Philip; Caspary, R Guy; Livingston, Brittney; Taylor, Lisa; Bigham, Kyle; Morris, Arvia E; Bailey, Robert W

    2015-01-01

    Pressures for cost-effective new therapies and an increased emphasis on emerging markets require technological advancements and a flexible future manufacturing network for the production of biologic medicines. The safety and efficacy of a product is crucial, and consistent product quality is an essential feature of any therapeutic manufacturing process. The active control of product quality in a typical biologic process is challenging because of measurement lags and nonlinearities present in the system. The current study uses nonlinear model predictive control to maintain a critical product quality attribute at a predetermined value during pilot scale manufacturing operations. This approach to product quality control ensures a more consistent product for patients, enables greater manufacturing efficiency, and eliminates the need for extensive process characterization by providing direct measures of critical product quality attributes for real time release of drug product. PMID:26179435

  12. Modeling the Philippines' real gross domestic product: A normal estimation equation for multiple linear regression

    NASA Astrophysics Data System (ADS)

    Urrutia, Jackie D.; Tampis, Razzcelle L.; Mercado, Joseph; Baygan, Aaron Vito M.; Baccay, Edcon B.

    2016-02-01

    The objective of this research is to formulate a mathematical model for the Philippines' Real Gross Domestic Product (Real GDP). The following factors are considered: Consumers' Spending (x1), Government's Spending (x2), Capital Formation (x3) and Imports (x4) as the Independent Variables that can actually influence in the Real GDP in the Philippines (y). The researchers used a Normal Estimation Equation using Matrices to create the model for Real GDP and used α = 0.01.The researchers analyzed quarterly data from 1990 to 2013. The data were acquired from the National Statistical Coordination Board (NSCB) resulting to a total of 96 observations for each variable. The data have undergone a logarithmic transformation particularly the Dependent Variable (y) to satisfy all the assumptions of the Multiple Linear Regression Analysis. The mathematical model for Real GDP was formulated using Matrices through MATLAB. Based on the results, only three of the Independent Variables are significant to the Dependent Variable namely: Consumers' Spending (x1), Capital Formation (x3) and Imports (x4), hence, can actually predict Real GDP (y). The regression analysis displays that 98.7% (coefficient of determination) of the Independent Variables can actually predict the Dependent Variable. With 97.6% of the result in Paired T-Test, the Predicted Values obtained from the model showed no significant difference from the Actual Values of Real GDP. This research will be essential in appraising the forthcoming changes to aid the Government in implementing policies for the development of the economy.

  13. Part 3 of a 4-part series Lip and Common Dental Care Products: Trends and Alternatives

    PubMed Central

    Jacob, Sharon; Katta, Rajani; Nedorost, Susan; Warshaw, Erin; Zirwas, Matt; Kruk, Ann

    2011-01-01

    Objective: To provide updated data on the use of ingredients that are common potential contact allergens in several categories of lip and oral products. To identify useful alternative products with few or no common contact allergens. Design: In November 2009, the full ingredient lists of 5,416 skin, hair, and cosmetic products marketed by the CVS pharmacy chain was copied from CVS.com into Microsoft Word format for analysis. Computer searches were made in Microsoft Word using search/replace and sorting functions to accurately identify the presence of specific allergens in each website product. Measurements: Percentages of American Contact Dermatitis Society core series allergens (and other common preservatives) were calculated. Results: The use of American Contact Dermatitis Society core series allergens (and other preservatives) in various categories of lip and oral products are reported. Conclusion: Data on allergens and alternatives for lip and oral products is not widely published. This article reviews some of the common potential allergens in lip liners, lipsticks, lip moisturizers, mouthwashes, and toothpastes. Suitable available alternative products for patients with contact allergy are listed. PMID:21938270

  14. DISCOVER Near Real-Time Ocean Data Products: Examples of Uses and Limitations

    NASA Astrophysics Data System (ADS)

    Smith, D. K.; Wentz, F. J.; Gentemann, C. L.

    2010-12-01

    Scientists at Remote Sensing Systems produce satellite microwave ocean data products for DISCOVER, a NASA MEaSUREs project. These ocean products include ocean surface winds, sea surface temperatures, atmospheric water vapor, cloud liquid water and rain rates created from passive microwave instrument data including that from SSM/I, TMI, AMSR-E, SSMIS and WindSat. Many users download DISCOVER data products in near real-time and use them within their specific applications. For example, some of the applications include ocean weather assessment for military sortie determination, sea surface temperature front identification for use by fishermen, and whale and turtle habitat monitoring. We will present the processing steps used to create the near real-time DISCOVER data, show several examples of user applications and discuss the benefits and limitations users find in applying our data products to their needs.

  15. Separating nitrogen fertilizer and irrigation water application in an alternating furrow irrigation system for maize production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The efficient use of water and nitrogen represents a primary concern to agricultural production in northwest of China. A two-year field experiment was conducted to assess and model the interactive effects between water and nitrogen (N) on maize (Zea mays L.) when grown with alternating furrow irriga...

  16. Viable Alternative Substrate Components for Use in Nursery and Greenhouse Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Peat-based container substrates are widely used for greenhouse crop production. A variety of alternative materials have been evaluated due to uncertainty over the cost and availability of Canadian peat moss. Processed whole pine trees (WPT) have been identified as a replacement for peat due to the w...

  17. Effect of fertilization and irrigation on nursery production of hydrangea using alternative containers

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Container production of nursery plants using biodegradable containers has been studied in recent years as a sustainable alternative to traditional plastic containers. Plant growth and photosynthetic performance of Hydrangea macrophylla ‘Merritt’s Supreme’ were investigated in this study when they we...

  18. Youth Attitudes and Beliefs toward Alternative Tobacco Products: Cigars, Bidis, and Kreteks

    ERIC Educational Resources Information Center

    Soldz, Stephen; Dorsey, Elizabeth

    2005-01-01

    Youth use of cigars, bidis, and kreteks has spread as youth cigarette use has declined. This study investigates young people's attitudes toward and beliefs about these alternative tobacco products. The study used data from a convenience sample survey of 5,016 7th-through 12th-grade students in Massachusetts. The cigar attitudes receiving the…

  19. Next day Salmonella spp. detection method based on real-time PCR for meat, dairy and vegetable food products.

    PubMed

    Rodriguez-Lazaro, David; Gonzalez-García, Patricia; Delibato, Elisabetta; De Medici, Dario; García-Gimeno, Rosa Maria; Valero, Antonio; Hernandez, Marta

    2014-08-01

    The microbiological standard for detection of Salmonella relies on several cultural steps and requires more than 5 days for final confirmation, and as consequence there is a need for an alternative rapid methodology for its detection. The aim of this study was to compare different detection strategies based on real-time PCR for a rapid and sensitive detection in an ample range of food products: raw pork and poultry meat, ready to eat lettuce salad and raw sheep milk cured cheese. Three main parameters were evaluated to reduce the time and cost for final results: the initial sample size (25 and 50 g), the incubation times (6, 10 and 18 h) and the bacterial DNA extraction (simple boiling of the culture after washing the bacterial pellet, the use of the Chelex resin, and a commercial silica column). The results obtained demonstrate that a combination of an incubation in buffered peptone water for 18 h of a 25 g-sample coupled to a DNA extraction by boiling and a real-time PCR assay detected down to 2-4 Salmonella spp.CFU per sample in less than 21 h in different types of food products. This RTi-PCR-based method is fully compatible with the ISO standard, providing results more rapidly and cost-effectively. The results were confirmed in a large number of naturally contaminated food samples with at least the same analytical performance as the reference method. PMID:24713474

  20. Effects of Real-Time Cochlear Implant Simulation on Speech Perception and Production

    ERIC Educational Resources Information Center

    Casserly, Elizabeth D.

    2013-01-01

    Real-time use of spoken language is a fundamentally interactive process involving speech perception, speech production, linguistic competence, motor control, neurocognitive abilities such as working memory, attention, and executive function, environmental noise, conversational context, and--critically--the communicative interaction between…

  1. Real-Time IGS products verification in the context of their use in Precise Point Positioning

    NASA Astrophysics Data System (ADS)

    Hadas, Tomasz; Bosy, Jaroslaw; Kaplon, Jan; Sierny, Jan

    2013-04-01

    Precise Point Positioning (PPP) is a positioning technique of single GNSS receiver which applies high quality products from permanent GNSS observations to utilize the computational potential of global network analysis. Estimated satellite orbits and clocks corrections are introduced into equation system as known parameters. PPP requires the application of precise products, since their quality directly reflects the positioning accuracy. In June 2007 IGS Real-time Pilot Project has started in order to satisfy real-time users, which is especially important for Precise Point Positioning. Currently available streams including precise orbits, clocks and code biases are standardized on RTCM-SC 104 formats and may be used as a substitute for ultra-rapid products. The target combination product performances are 0.3ns for satellite clock accuracy and orbit accuracy at the level of the IGS Ultra predictions with maximum latency of 10s. This study presents the quality assessment of currently available Real-Time IGS products. Long-term test include comparisons of disseminated information with final and high-rate products, stability assessment over time, as well as latency validation of available RTCM streams.

  2. Development of a high-throughput real time PCR based on a hot-start alternative for Pfu mediated by quantum dots

    NASA Astrophysics Data System (ADS)

    Sang, Fuming; Yang, Yang; Yuan, Lin; Ren, Jicun; Zhang, Zhizhou

    2015-09-01

    Hot start (HS) PCR is an excellent alternative for high-throughput real time PCR due to its ability to prevent nonspecific amplification at low temperature. Development of a cost-effective and simple HS PCR technique to guarantee high-throughput PCR specificity and consistency still remains a great challenge. In this study, we systematically investigated the HS characteristics of QDs triggered in real time PCR with EvaGreen and SYBR Green I dyes by the analysis of amplification curves, standard curves and melting curves. Two different kinds of DNA polymerases, Pfu and Taq, were employed. Here we showed that high specificity and efficiency of real time PCR were obtained in a plasmid DNA and an error-prone two-round PCR assay using QD-based HS PCR, even after an hour preincubation at 50 °C before real time PCR. Moreover, the results obtained by QD-based HS PCR were comparable to a commercial Taq antibody DNA polymerase. However, no obvious HS effect of QDs was found in real time PCR using Taq DNA polymerase. The findings of this study demonstrated that a cost-effective high-throughput real time PCR based on QD triggered HS PCR could be established with high consistency, sensitivity and accuracy.Hot start (HS) PCR is an excellent alternative for high-throughput real time PCR due to its ability to prevent nonspecific amplification at low temperature. Development of a cost-effective and simple HS PCR technique to guarantee high-throughput PCR specificity and consistency still remains a great challenge. In this study, we systematically investigated the HS characteristics of QDs triggered in real time PCR with EvaGreen and SYBR Green I dyes by the analysis of amplification curves, standard curves and melting curves. Two different kinds of DNA polymerases, Pfu and Taq, were employed. Here we showed that high specificity and efficiency of real time PCR were obtained in a plasmid DNA and an error-prone two-round PCR assay using QD-based HS PCR, even after an hour

  3. Portable inference engine: An extended CLIPS for real-time production systems

    NASA Technical Reports Server (NTRS)

    Le, Thach; Homeier, Peter

    1988-01-01

    The present C-Language Integrated Production System (CLIPS) architecture has not been optimized to deal with the constraints of real-time production systems. Matching in CLIPS is based on the Rete Net algorithm, whose assumption of working memory stability might fail to be satisfied in a system subject to real-time dataflow. Further, the CLIPS forward-chaining control mechanism with a predefined conflict resultion strategy may not effectively focus the system's attention on situation-dependent current priorties, or appropriately address different kinds of knowledge which might appear in a given application. Portable Inference Engine (PIE) is a production system architecture based on CLIPS which attempts to create a more general tool while addressing the problems of real-time expert systems. Features of the PIE design include a modular knowledge base, a modified Rete Net algorithm, a bi-directional control strategy, and multiple user-defined conflict resolution strategies. Problems associated with real-time applications are analyzed and an explanation is given for how the PIE architecture addresses these problems.

  4. Water consumption footprint and land requirements of large-scale alternative diesel and jet fuel production.

    PubMed

    Staples, Mark D; Olcay, Hakan; Malina, Robert; Trivedi, Parthsarathi; Pearlson, Matthew N; Strzepek, Kenneth; Paltsev, Sergey V; Wollersheim, Christoph; Barrett, Steven R H

    2013-01-01

    Middle distillate (MD) transportation fuels, including diesel and jet fuel, make up almost 30% of liquid fuel consumption in the United States. Alternative drop-in MD and biodiesel could potentially reduce dependence on crude oil and the greenhouse gas intensity of transportation. However, the water and land resource requirements of these novel fuel production technologies must be better understood. This analysis quantifies the lifecycle green and blue water consumption footprints of producing: MD from conventional crude oil; Fischer-Tropsch MD from natural gas and coal; fermentation and advanced fermentation MD from biomass; and hydroprocessed esters and fatty acids MD and biodiesel from oilseed crops, throughout the contiguous United States. We find that FT MD and alternative MD derived from rainfed biomass have lifecycle blue water consumption footprints of 1.6 to 20.1 Lwater/LMD, comparable to conventional MD, which ranges between 4.1 and 7.4 Lwater/LMD. Alternative MD derived from irrigated biomass has a lifecycle blue water consumption footprint potentially several orders of magnitude larger, between 2.7 and 22 600 Lwater/LMD. Alternative MD derived from biomass has a lifecycle green water consumption footprint between 1.1 and 19 200 Lwater/LMD. Results are disaggregated to characterize the relationship between geo-spatial location and lifecycle water consumption footprint. We also quantify the trade-offs between blue water consumption footprint and areal MD productivity, which ranges from 490 to 4200 LMD/ha, under assumptions of rainfed and irrigated biomass cultivation. Finally, we show that if biomass cultivation for alternative MD is irrigated, the ratio of the increase in areal MD productivity to the increase in blue water consumption footprint is a function of geo-spatial location and feedstock-to-fuel production pathway. PMID:24066845

  5. Green Alternatives to Nitrates and Nitrites in Meat-based Products-A Review.

    PubMed

    Gassara, Fatma; Kouassi, Anne Patricia; Brar, Satinder Kaur; Belkacemi, Khaled

    2016-10-01

    Several food additives are added in food for their preservation to maintain the freshness of food (antioxidants) or to slow down or stop the growth of microorganisms (preservative agents). Nitrites and nitrates are used as preservative agents in meat. Nitrites give a smoked taste, a pinkish color in the meat and protect the consumers against the risk of bacterial deterioration. Their addition is however very limited as, in high dose, it can have risks on human health and the environment. Nitrites may also combine with secondary or tertiary amines to form N-nitroso derivatives. Certain N-nitroso compounds have been shown to produce cancers in a wide range of laboratory animals. Thus, alternatives of nitrates and nitrites are the object of numerous research studies. Alternatives, such as the addition of vitamins, fruits, chemicals products, natural products containing nitrite or spices, which have similar properties of nitrites, are in evaluation. In fact, spices are considered to have several organoleptic and anti-microbial properties which would be interesting to study. Several spices and combinations of spices are being progressively evaluated. This review discusses the sources of nitrites and nitrates, their use as additives in food products, their physicochemical properties, their negatives effects and the use of alternatives of nitrites and nitrates in preserving meat products. PMID:25750989

  6. Cheese whey: A cost-effective alternative for hyaluronic acid production by Streptococcus zooepidemicus.

    PubMed

    Amado, Isabel R; Vázquez, José A; Pastrana, Lorenzo; Teixeira, José A

    2016-05-01

    This study focuses on the optimisation of cheese whey formulated media for the production of hyaluronic acid (HA) by Streptococcus zooepidemicus. Culture media containing whey (W; 2.1g/L) or whey hydrolysate (WH; 2.4 g/L) gave the highest HA productions. Both W and WH produced high yields on protein consumed, suggesting cheese whey is a good nitrogen source for S. zooepidemicus production of HA. Polysaccharide concentrations of 4.0 g/L and 3.2g/L were produced in W and WH in a further scale-up to 5L bioreactors, confirming the suitability of the low-cost nitrogen source. Cheese whey culture media provided high molecular weight (>3000 kDa) HA products. This study revealed replacing the commercial peptone by the low-cost alternative could reduce HA production costs by up to a 70% compared to synthetic media. PMID:26769504

  7. Using Distributional Statistics to Acquire Morphophonological Alternations: Evidence from Production and Perception

    PubMed Central

    Buckler, Helen; Fikkert, Paula

    2016-01-01

    Morphophonological alternations, such as the voicing alternation that arises in a morphological paradigm due to final-devoicing in Dutch, are notoriously difficult for children to acquire. This has previously been attributed to their unpredictability. In fact, the presence or absence of a voicing alternation is partly predictable if the phonological context of the word is taken into account, and adults have been shown to use this information (Ernestus and Baayen, 2003). This study investigates whether voicing alternations are predictable from the child’s input, and whether children can make use of this information. A corpus study of child-directed speech establishes that the likelihood of a stem-final obstruent alternating is somewhat predictable on the basis of the phonological properties of the stem. In Experiment 1 Dutch 3-year-olds’ production accuracy in a plural-elicitation task is shown to be sensitive to the distributional statistics. However, distributional properties do not play a role in children’s sensitivity to mispronunciations of voicing in a Preferential Looking Task in Experiment 2. PMID:27199792

  8. Metabolic engineering of Corynebacterium glutamicum aimed at alternative carbon sources and new products.

    PubMed

    Zahoor, Ahmed; Lindner, Steffen N; Wendisch, Volker F

    2012-01-01

    Corynebacterium glutamicum is well known as the amino acid-producing workhorse of fermentation industry, being used for multi-million-ton scale production of glutamate and lysine for more than 60 years. However, it is only recently that extensive research has focused on engineering it beyond the scope of amino acids. Meanwhile, a variety of corynebacterial strains allows access to alternative carbon sources and/or allows production of a wide range of industrially relevant compounds. Some of these efforts set new standards in terms of titers and productivities achieved whereas others represent a proof-of-principle. These achievements manifest the position of C. glutamicum as an important industrial microorganism with capabilities far beyond the traditional amino acid production. In this review we focus on the state of the art of metabolic engineering of C. glutamicum for utilization of alternative carbon sources, (e.g. coming from wastes and unprocessed sources), and construction of C. glutamicum strains for production of new products such as diamines, organic acids and alcohols. PMID:24688664

  9. Knowledge and acceptability of alternative HIV prevention bio-medical products among MSM who bareback.

    PubMed

    Nodin, N; Carballo-Diéguez, A; Ventuneac, A M; Balan, I C; Remien, R

    2008-01-01

    Condom use is the best available strategy to prevent HIV infection during sexual intercourse. However, since many people choose not to use condoms in circumstances in which HIV risk exists, alternatives to condom use for HIV prevention are needed. Currently there are several alternative bio-medical HIV-prevention products in different stages of development: microbicides, vaccines, post-exposure prophylaxis (PEP) and pre-exposure prophylaxis (PrEP). Seventy-two men who have sex with men (MSM) who took part in a study on Internet use and intentional condomless anal intercourse were asked about these four products during a semi-structured interview. The questions explored knowledge and acceptability of all the products and willingness to participate in microbicide and vaccine trials. Qualitative analysis of the data suggests that these men had virtually no knowledge of PrEP, very limited knowledge of microbicides, some information about PEP and considerably more knowledge about vaccines. Reactions towards the products were generally positive except for PrEP, for which reactions were polarized as either enthusiastic or negative. With the exception of PrEP, many men expressed willingness to use the products in the future. Most men would be willing to participate in trials for microbicides and vaccines if given basic reassurances. Concerns over negative side effects and preoccupation with possible infection were some of the motives given for non-willingness to participate in a vaccine trial. These results should inform the development of future trials of biomedical prevention products. PMID:18278621

  10. Metabolic engineering of Corynebacterium glutamicum aimed at alternative carbon sources and new products

    PubMed Central

    Zahoor, Ahmed; Lindner, Steffen N.; Wendisch, Volker F.

    2012-01-01

    Corynebacterium glutamicum is well known as the amino acid-producing workhorse of fermentation industry, being used for multi-million-ton scale production of glutamate and lysine for more than 60 years. However, it is only recently that extensive research has focused on engineering it beyond the scope of amino acids. Meanwhile, a variety of corynebacterial strains allows access to alternative carbon sources and/or allows production of a wide range of industrially relevant compounds. Some of these efforts set new standards in terms of titers and productivities achieved whereas others represent a proof-of-principle. These achievements manifest the position of C. glutamicum as an important industrial microorganism with capabilities far beyond the traditional amino acid production. In this review we focus on the state of the art of metabolic engineering of C. glutamicum for utilization of alternative carbon sources, (e.g. coming from wastes and unprocessed sources), and construction of C. glutamicum strains for production of new products such as diamines, organic acids and alcohols PMID:24688664

  11. Identification of pork in meat products using real-time loop-mediated isothermal amplification

    PubMed Central

    Yang, Lixia; Fu, Shujun; Peng, Xinkai; Li, Le; Song, Taoping; Li, Lin

    2014-01-01

    In this study, a one-step, real-time, loop-mediated isothermal amplification (RealAmp) assay was developed, for the highly specific detection of pork DNA. For the assay, the mtDNA of cytochrome b (cytb) gene was amplified at 63 °C using SYBR Green I for 45 min with a Real-Time Polymerase Chain Reaction (PCR) System that measured the fluorescent signal at one-minute intervals. As little as 1 pg of template DNA could be detected, without any cross-reactivity with non-target species. Meat mixtures, heat-treated at 100 °C for 15 min, prepared by mixing pork meat with beef at different ratios (0.01%–10%) were tested, and the RealAmp assays allowed the detection of as little as 0.01% pork in the meat mixtures. Thus, this work showed that RealAmp could be used for specific identification and sensitive quantification of meat species, even for heat-treated meat products. PMID:26019573

  12. 75 FR 71155 - Market Test of Experimental Product: “Alternative Postage Payment Method for Greeting Cards”

    Federal Register 2010, 2011, 2012, 2013, 2014

    2010-11-22

    ... From the Federal Register Online via the Government Publishing Office POSTAL SERVICE Market Test of Experimental Product: ``Alternative Postage Payment Method for Greeting Cards'' AGENCY: Postal... Postage Payment Method for Greeting Cards'' experimental product on January 2, 2011. The Postal...

  13. Genetically encoded sensors enable real-time observation of metabolite production.

    PubMed

    Rogers, Jameson K; Church, George M

    2016-03-01

    Engineering cells to produce valuable metabolic products is hindered by the slow and laborious methods available for evaluating product concentration. Consequently, many designs go unevaluated, and the dynamics of product formation over time go unobserved. In this work, we develop a framework for observing product formation in real time without the need for sample preparation or laborious analytical methods. We use genetically encoded biosensors derived from small-molecule responsive transcription factors to provide a fluorescent readout that is proportional to the intracellular concentration of a target metabolite. Combining an appropriate biosensor with cells designed to produce a metabolic product allows us to track product formation by observing fluorescence. With individual cells exhibiting fluorescent intensities proportional to the amount of metabolite they produce, high-throughput methods can be used to rank the quality of genetic variants or production conditions. We observe production of several renewable plastic precursors with fluorescent readouts and demonstrate that higher fluorescence is indeed an indicator of higher product titer. Using fluorescence as a guide, we identify process parameters that produce 3-hydroxypropionate at 4.2 g/L, 23-fold higher than previously reported. We also report, to our knowledge, the first engineered route from glucose to acrylate, a plastic precursor with global sales of $14 billion. Finally, we monitor the production of glucarate, a replacement for environmentally damaging detergents, and muconate, a renewable precursor to polyethylene terephthalate and nylon with combined markets of $51 billion, in real time, demonstrating that our method is applicable to a wide range of molecules. PMID:26858408

  14. Genetically encoded sensors enable real-time observation of metabolite production

    PubMed Central

    Rogers, Jameson K.; Church, George M.

    2016-01-01

    Engineering cells to produce valuable metabolic products is hindered by the slow and laborious methods available for evaluating product concentration. Consequently, many designs go unevaluated, and the dynamics of product formation over time go unobserved. In this work, we develop a framework for observing product formation in real time without the need for sample preparation or laborious analytical methods. We use genetically encoded biosensors derived from small-molecule responsive transcription factors to provide a fluorescent readout that is proportional to the intracellular concentration of a target metabolite. Combining an appropriate biosensor with cells designed to produce a metabolic product allows us to track product formation by observing fluorescence. With individual cells exhibiting fluorescent intensities proportional to the amount of metabolite they produce, high-throughput methods can be used to rank the quality of genetic variants or production conditions. We observe production of several renewable plastic precursors with fluorescent readouts and demonstrate that higher fluorescence is indeed an indicator of higher product titer. Using fluorescence as a guide, we identify process parameters that produce 3-hydroxypropionate at 4.2 g/L, 23-fold higher than previously reported. We also report, to our knowledge, the first engineered route from glucose to acrylate, a plastic precursor with global sales of $14 billion. Finally, we monitor the production of glucarate, a replacement for environmentally damaging detergents, and muconate, a renewable precursor to polyethylene terephthalate and nylon with combined markets of $51 billion, in real time, demonstrating that our method is applicable to a wide range of molecules. PMID:26858408

  15. Perspectives and strategies of alternative methods used in the risk assessment of personal care products.

    PubMed

    Quantin, P; Thélu, A; Catoire, S; Ficheux, H

    2015-11-01

    Risk assessment for personal care products requires the use of alternative methods since animal testing is now totally banned. Some of these methods are effective and have been validated by the "European Union Reference Laboratory for alternatives to animal testing"; but there is still a need for development and implementation of methods for specific endpoints. In this review, we have focused on dermal risk assessment because it is the prime route of absorption and main target organ for personal care products. Within this field, various areas must be assessed: irritation, sensitisation and toxicokinetic. Personal care product behaviour after use by the consumer and potential effects on the environment are also discussed. The purpose of this review is to show evolution and the prospects of alternative methods for safety dermal assessment. Assessment strategies must be adapted to the different chemical classes of substances studied but also to the way in which they are used. Finally, experimental and theoretical technical parameters that may impact on measured effects have been identified and discussed. PMID:26184446

  16. New Products for Near Real-Time Enhanced Landslide Identification and Precipitation Monitoring

    NASA Astrophysics Data System (ADS)

    Roberts-Pierel, J.; Ahamed, A.; Fayne, J.; Rumsey, A.

    2015-12-01

    Nepal and the Himalayan region are hotspots for landslide activity due to mountainous topography, complex terrain, and monsoon rains. Current research in landslide modeling and detection generally requires high resolution imagery with software aided classification or manual digitization by analysts. These methods are plagued by low spatial and temporal accuracy. Addressing issues in conventional measurement, this study combined optical data from Landsat 8, a Digital Elevation Model (DEM) generated from Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), and precipitation data from the Global Precipitation Measurement Mission (GPM) to create two products. The Sudden Landslide Identification Product (SLIP) uses Landsat 8 and the ASTER DEM to identify landslides in near real-time, and provides damage assessments by mapping landslides triggered by precipitation. Detecting Real-time Increased Precipitation (DRIP) monitors precipitation levels extracted from the GPM-IMERG 30-minute product to create alerts in near real-time when current rainfall levels exceed regional threshold values. After a landslide detection is made by SLIP, historical rainfall data from DRIP is analyzed to estimate a date for the detected landslide. Together, DRIP and SLIP will be used by local and regional organizations in Nepal such as the International Centre for Integrated Mountain Development (ICIMOD), as well as the international scientific community to protect lives, preserve infrastructure, and manage local ecosystems.

  17. An alternative feedstock of corn meal for industrial fuel ethanol production: delignified corncob residue.

    PubMed

    Lei, Cheng; Zhang, Jian; Xiao, Lin; Bao, Jie

    2014-09-01

    Delignified corncob residue is an industrial solid waste from xylose production using corncob as feedstock. In this study, delignified corncob residue was used as the feedstock of ethanol production by simultaneous saccharification and fermentation (SSF) and the optimal fermentation performance was investigated under various operation conditions. The ethanol titer and yield reached 75.07 g/L and 89.38%, respectively, using a regular industrial yeast strain at moderate cellulase dosage and high solids loading. A uniform SSF temperature of 37°C at both prehydrolysis and SSF stages was tested. The fermentation performance and cost of delignified corncob residue and corn meal was compared as feedstock of ethanol fermentation. The result shows that the delignified corncob residue is competitive to corn meal as ethanol production feedstock. The study gives a typical case to demonstrate the potential of intensively processed lignocellulose as the alternative feedstock of corn meal for industrial fuel ethanol production. PMID:25027810

  18. Use of Alternative Tobacco Products in Multiethnic Youth from Jujuy, Argentina

    PubMed Central

    Alderete, Ethel; Kaplan, Celia Patricia; Gregorich, Steven E.; Pérez-Stable, Eliseo J.

    2010-01-01

    This study examines alternative tobacco use among Latin American youth. A self-administered survey in a random sample of 27 schools was administered in 2004 in Jujuy, Argentina (N = 3218). Prevalence of alternative tobacco product use was 24.1%; 15.3% of youth used hand-rolled cigarettes, 7.8% smoked cigars, 2.3% chewed tobacco leaf and 1.6% smoked pipe. Among youth who never smoked manufactured cigarettes, alternative product use was rare (2.9%), except for chewing tobacco (22%). In multivariate logistic regression boys were more likely than girls to smoke pipe (OR = 3.1; 95% CI 1.1–8.7); indigenous language was associated with smoking hand-rolled cigarettes (OR = 1.4; 95% CI-1.1–1.9) and pipe (OR = 2.2; 95% CI 1.5–3.4). Working in tobacco sales was a risk factor for chewing tobacco (OR = 2.9; 95% CI: 1.7–4.9) and smoking hand-rolled cigarettes (OR = 1.4; 95% CI 1.1–1.8). Having friends who smoked was associated with chewing tobacco (OR = 1.8; 95% CI 1.0–3.2) and with smoking cigars (OR = 2.1; 95% CI 1.5–2.9). Current drinking and thrill-seeking orientation were associated with cigars and pipe smoking. Findings highlight the importance of surveillance of alternative tobacco products use and availability among youth and for addressing identified risk factors. PMID:20300454

  19. Global, real-time ionosphere specification for end-user communication and navigation products

    NASA Astrophysics Data System (ADS)

    Tobiska, W.; Carlson, H. C.; Schunk, R. W.; Thompson, D. C.; Sojka, J. J.; Scherliess, L.; Zhu, L.; Gardner, L. C.

    2010-12-01

    Space weather’s effects upon the near-Earth environment are due to dynamic changes in the energy transfer processes from the Sun’s photons, particles, and fields. Of the space environment domains that are affected by space weather, the ionosphere is the key region that affects communication and navigation systems. The Utah State University (USU) Space Weather Center (SWC) is a developer and producer of commercial space weather applications. A key system-level component for providing timely information about the effects of space weather is the Global Assimilation of Ionospheric Measurements (GAIM) system. GAIM, operated by SWC, improves real-time communication and navigation systems by continuously ingesting up to 10,000 slant TEC measurements every 15-minutes from approximately 500 stations. Using a Kalman filter, the background output from the physics-based Ionosphere Forecast Model (IFM) is adjusted to more accurately represent the actual ionosphere. An improved ionosphere leads to more useful derivative products. For example, SWC runs operational code, using GAIM, to calculate and report the global radio high frequency (HF) signal strengths for 24 world cities. This product is updated every 15 minutes at http://spaceweather.usu.edu and used by amateur radio operators. SWC also developed and provides through Apple iTunes the widely used real-time space weather iPhone app called SpaceWx for public space weather education. SpaceWx displays the real-time solar, heliosphere, magnetosphere, thermosphere, and ionosphere drivers to changes in the total electron content, for example. This smart phone app is tip of the “iceberg” of automated systems that provide space weather data; it permits instant understanding of the environment surrounding Earth as it dynamically changes. SpaceWx depends upon a distributed network that connects satellite and ground-based data streams with algorithms to quickly process the measurements into geophysical data, incorporate those

  20. Reduction of Biological Sludge Production Applying an Alternating Oxic/anoxic Process in Water Line.

    PubMed

    Eusebi, Anna Laura; Panigutti, Maximiliano; Battistoni, Paolo

    2016-06-01

    Alternating oxic/anoxic process, applied for the main objective of the improvement of nitrogen performances, was studied in terms of secondary effect of biomass reduction. The process was carried out in one real water resource recovery facility and the data were compared with the previous conventional period when a conventional process was adopted. The main mechanism of the process for the sludge minimization is recognized in the metabolic uncoupling. In fact, an increase of the specific oxygen uptake rate in the biological reactor was recorded stimulated by the change of the oxidation reduction potential environment. Moreover, the heterotrophic growth yield was measured equal to 0.385 kgVSS/kgCOD. The global percentage of reduction was tested with the mass balance of solids. The process is able to decrease the observed sludge yield up to 20%. The specific energy consumption was evaluated. PMID:27225780

  1. Water productivity of stacked and alternate-year rotations under conventional and ecological management.

    NASA Astrophysics Data System (ADS)

    Allen, Brett; Lenssen, Andrew; Jabro, Jay; Iversen, Bill; Sainju, Upendra

    2015-04-01

    Dryland cropping system productivity in the North America northern Great Plains (NGP) likely can benefit from diversification of the traditional wheat-summer fallow system through improvements in crop sequence and level of management. A long-term study initiated in 2004 near Culbertson, Montana, U.S.A. compared the impact of rotation type (Stacked vs. Alternate-year) and management level (Conventional vs. Ecological) on canola, flax, and pea grown in rotation with durum. Ecological management included no-till, N fertilizer banded at planting, greater seeding rate, and taller stubble height. Conventional management included single pass tillage with sweeps in spring, fertilizer N broadcast just before planting, standard seeding rate, and standard stubble height. For years 2008-2010, a 13% increase in durum grain and pea seed yield in Ecological than Conventional management was not related to water use or water productivity of grain. Similarly, an 18% increase in pea seed yield in alternate-year rotations (e.g. durum-pea-durum-flax) than stacked rotations (e.g. durum-durum-flax-pea) was not related to water use or water productivity of seed. Water use, water productivity for grain, and water productivity for above ground biomass were similar for management level and rotation type within a given crop. Averaged across management level and rotation type, water use was 326, 351, 372, and 292 mm for canola, durum, flax, and pea. Likewise, water productivity for grain averaged 4.2, 6.5, 2.5, and 7.7 kg/ha/mm for canola, durum, flax, and pea. Water use and water productivity varied significantly among years, underscoring the need to development stable cropping systems that are less susceptible to the highly variable growing environment of the semi-arid NGP. Overall, Ecological management increased durum and pea yield, while Stacked rotations either decreased crop yield (pea) or offered no measurable advantage to Alternate-year rotations. Any differences in yield were not

  2. Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media.

    PubMed

    Jozala, Angela Faustino; Pértile, Renata Aparecida Nedel; dos Santos, Carolina Alves; de Carvalho Santos-Ebinuma, Valéria; Seckler, Marcelo Martins; Gama, Francisco Miguel; Pessoa, Adalberto

    2015-02-01

    Bacterial cellulose (BC) is used in different fields as a biological material due to its unique properties. Despite there being many BC applications, there still remain many problems associated with bioprocess technology, such as increasing productivity and decreasing production cost. New technologies that use waste from the food industry as raw materials for culture media promote economic advantages because they reduce environmental pollution and stimulate new research for science sustainability. For this reason, BC production requires optimized conditions to increase its application. The main objective of this study was to evaluate BC production by Gluconacetobacter xylinus using industry waste, namely, rotten fruits and milk whey, as culture media. Furthermore, the structure of BC produced at different conditions was also determined. The culture media employed in this study were composed of rotten fruit collected from the disposal of free markets, milk whey from a local industrial disposal, and their combination, and Hestrin and Schramm media was used as standard culture media. Although all culture media studied produced BC, the highest BC yield-60 mg/mL-was achieved with the rotten fruit culture. Thus, the results showed that rotten fruit can be used for BC production. This culture media can be considered as a profitable alternative to generate high-value products. In addition, it combines environmental concern with sustainable processes that can promote also the reduction of production cost. PMID:25472434

  3. Web Based Access to Real-Time Meteorological Products Optimized for PDA- Smartphones

    NASA Astrophysics Data System (ADS)

    Dengel, R. C.; Bellon, W.; Robaidek, J.

    2006-05-01

    Recent advances in wireless broadband services and coverage have made access to the internet possible in remote locations. Users can now access the web via an ever increasing number of small, handheld devices specifically designed to allow voice and data exchange using this expanding service. So called PDA phones or smartphones blend the features of traditional PDA devices with telecommunications capabilities. The University of Wisconsin - Madison, Space Science and Engineering Center (SSEC) has produced a web site holding a variety of meteorological image and text displays optimized for this new technology. The site features animations of real-time radar and satellite clouds with value added graphical overlays of severe watches and warnings. Products focus on remotely sensed information supplemented with conventional ground observations. The PDA Animated Weather (PAW) website has rapidly been adopted by numerous institutions and individuals desiring access to real-time meteorological information independent of their location. Of particular note are users that can be classified as first responders, including foreign and domestic based police and file departments. This paper offers an overview of the PAW project including product design, automated production and web presentation. Numerous examples of user applications will be presented, planned future products and functionality will be discussed.

  4. Listening to Puns Elicits the Co-Activation of Alternative Homophone Meanings during Language Production.

    PubMed

    Rose, Sebastian Benjamin; Spalek, Katharina; Rahman, Rasha Abdel

    2015-01-01

    Recent evidence suggests that lexical-semantic activation spread during language production can be dynamically shaped by contextual factors. In this study we investigated whether semantic processing modes can also affect lexical-semantic activation during word production. Specifically, we tested whether the processing of linguistic ambiguities, presented in the form of puns, has an influence on the co-activation of unrelated meanings of homophones in a subsequent language production task. In a picture-word interference paradigm with word distractors that were semantically related or unrelated to the non-depicted meanings of homophones we found facilitation induced by related words only when participants listened to puns before object naming, but not when they heard jokes with unambiguous linguistic stimuli. This finding suggests that a semantic processing mode of ambiguity perception can induce the co-activation of alternative homophone meanings during speech planning. PMID:26114942

  5. Listening to Puns Elicits the Co-Activation of Alternative Homophone Meanings during Language Production

    PubMed Central

    Rose, Sebastian Benjamin; Spalek, Katharina; Rahman, Rasha Abdel

    2015-01-01

    Recent evidence suggests that lexical-semantic activation spread during language production can be dynamically shaped by contextual factors. In this study we investigated whether semantic processing modes can also affect lexical-semantic activation during word production. Specifically, we tested whether the processing of linguistic ambiguities, presented in the form of puns, has an influence on the co-activation of unrelated meanings of homophones in a subsequent language production task. In a picture-word interference paradigm with word distractors that were semantically related or unrelated to the non-depicted meanings of homophones we found facilitation induced by related words only when participants listened to puns before object naming, but not when they heard jokes with unambiguous linguistic stimuli. This finding suggests that a semantic processing mode of ambiguity perception can induce the co-activation of alternative homophone meanings during speech planning. PMID:26114942

  6. Biohydrogen and biomethane production sustained by untreated matrices and alternative application of compost waste.

    PubMed

    Arizzi, Mariaconcetta; Morra, Simone; Pugliese, Massimo; Gullino, Maria Lodovica; Gilardi, Gianfranco; Valetti, Francesca

    2016-10-01

    Biohydrogen and biomethane production offers many advantages for environmental protection over the fossil fuels or the existing physical-chemical methods for hydrogen and methane synthesis. The aim of this study is focused on the exploitation of several samples from the composting process: (1) a mixture of waste vegetable materials ("Mix"); (2) an unmatured compost sample (ACV15); and (3) three types of green compost with different properties and soil improver quality (ACV1, ACV2 and ACV3). These samples were tested for biohydrogen and biomethane production, thus obtaining second generation biofuels and resulting in a novel possibility to manage renewable waste biomasses. The ability of these substrates as original feed during dark fermentation was assayed anaerobically in batch, in glass bottles, in order to determine the optimal operating conditions for hydrogen and/or methane production using "Mix" or ACV1, ACV2 or ACV3 green compost and a limited amount of water. Hydrogen could be produced with a fast kinetic in the range 0.02-2.45mLH2g(-1)VS, while methane was produced with a slower kinetic in the range 0.5-8mLCH4g(-1)VS. It was observed that the composition of each sample influenced significantly the gas production. It was also observed that the addition of different water amounts play a crucial role in the development of hydrogen or methane. This parameter can be used to push towards the alternative production of one or another gas. Hydrogen and methane production was detected spontaneously from these matrices, without additional sources of nutrients or any pre-treatment, suggesting that they can be used as an additional inoculum or feed into single or two-stage plants. This might allow the use of compost with low quality as soil improver for alternative and further applications. PMID:27422046

  7. Real-Time Data Processing Systems and Products at the Alaska Earthquake Information Center

    NASA Astrophysics Data System (ADS)

    Ruppert, N. A.; Hansen, R. A.

    2007-05-01

    The Alaska Earthquake Information Center (AEIC) receives data from over 400 seismic sites located within the state boundaries and the surrounding regions and serves as a regional data center. In 2007, the AEIC reported ~20,000 seismic events, with the largest event of M6.6 in Andreanof Islands. The real-time earthquake detection and data processing systems at AEIC are based on the Antelope system from BRTT, Inc. This modular and extensible processing platform allows an integrated system complete from data acquisition to catalog production. Multiple additional modules constructed with the Antelope toolbox have been developed to fit particular needs of the AEIC. The real-time earthquake locations and magnitudes are determined within 2-5 minutes of the event occurrence. AEIC maintains a 24/7 seismologist-on-duty schedule. Earthquake alarms are based on the real- time earthquake detections. Significant events are reviewed by the seismologist on duty within 30 minutes of the occurrence with information releases issued for significant events. This information is disseminated immediately via the AEIC website, ANSS website via QDDS submissions, through e-mail, cell phone and pager notifications, via fax broadcasts and recorded voice-mail messages. In addition, automatic regional moment tensors are determined for events with M>=4.0. This information is posted on the public website. ShakeMaps are being calculated in real-time with the information currently accessible via a password-protected website. AEIC is designing an alarm system targeted for the critical lifeline operations in Alaska. AEIC maintains an extensive computer network to provide adequate support for data processing and archival. For real-time processing, AEIC operates two identical, interoperable computer systems in parallel.

  8. Name-letter branding under scrutiny: real products, new algorithms, and the probability of buying.

    PubMed

    Stieger, Stefan

    2010-06-01

    People like letters matching their own first and last name initials more than nonname letters. This name-letter effect has also been found for brands, i.e., people like brands resembling their own name letters (initial or first three). This has been termed name-letter branding effect. In the present study of 199 participants, ages 12 to 79 years, this name-letter branding effect was found for a modified design (1) using real products, (2) concentrating on product names rather than brand names, (3) using five different products for each letter of the Roman alphabet, (4) asking for the buying probability, and (5) using recently introduced algorithms, controlling for individual response tendencies (i.e., liking all letters more or less) and general normative popularity of particular letters (i.e., some letters are generally preferred more than other letters). PMID:20865997

  9. Soft expansion of double-real-virtual corrections to Higgs production at N3LO

    NASA Astrophysics Data System (ADS)

    Anastasiou, Charalampos; Duhr, Claude; Dulat, Falko; Furlan, Elisabetta; Herzog, Franz; Mistlberger, Bernhard

    2015-08-01

    We present methods to compute higher orders in the threshold expansion for the one-loop production of a Higgs boson in association with two partons at hadron colliders. This process contributes to the N3LO Higgs production cross section beyond the soft-virtual approximation. We use reverse unitarity to expand the phase-space integrals in the small kinematic parameters and to reduce the coefficients of the expansion to a small set of master integrals. We describe two methods for the calculation of the master integrals. The first was introduced for the calculation of the soft triple-real radiation relevant to N3LO Higgs production. The second uses a particular factorization of the three body phase-space measure and the knowledge of the scaling properties of the integral itself. Our result is presented as a Laurent expansion in the dimensional regulator, although some of the master integrals are computed to all orders in this parameter.

  10. Effects of real-time cochlear implant simulation on speech production.

    PubMed

    Casserly, Elizabeth D

    2015-05-01

    Investigations using normal-hearing subjects listening to simulations of cochlear implant (CI) acoustic processing have provided substantial information about the impact of these distorted listening conditions on the accuracy of auditory perception, but extensions of this method to the domain of speech production have been limited. In the present study, a portable, real-time vocoder was used to simulate conditions of CI auditory feedback during speech production in NH subjects. Acoustic-phonetic characteristics of sibilant fricatives, aspirated stops, and F1/F2 vowel qualities were analyzed for changes as a result of CI simulation of acoustic speech feedback. Significant changes specific to F1 were observed; speakers reduced their phonological vowel height contrast, typically via talker-specific raising of the low vowels [æ] and [ɑ] or lowering of high vowels [i] and [u]. Comparisons to the results of both localized feedback perturbation procedures and investigations of speech production in deaf adults with CIs are discussed. PMID:25994707

  11. The Protein Kinase A Pathway Regulates Zearalenone Production by Modulating Alternative ZEB2 Transcription.

    PubMed

    Park, Ae Ran; Fu, Minmin; Shin, Ji Young; Son, Hokyoung; Lee, Yin-Won

    2016-05-28

    Zearalenone (ZEA) is an estrogenic mycotoxin that is produced by several Fusarium species, including Fusarium graminearum. One of the ZEA biosynthetic genes, ZEB2, encodes two isoforms of Zeb2 by alternative transcription, forming an activator (Zeb2L-Zeb2L homooligomer) and an inhibitor (Zeb2L-Zeb2S heterodimer) that directly regulate the ZEA biosynthetic genes in F. graminearum. Cyclic AMP-dependent protein kinase A (PKA) signaling regulates secondary metabolic processes in several filamentous fungi. In this study, we investigated the effects of the PKA signaling pathway on ZEA biosynthesis. Through functional analyses of PKA catalytic and regulatory subunits (CPKs and PKR), we found that the PKA pathway negatively regulates ZEA production. Genetic and biochemical evidence further demonstrated that the PKA pathway specifically represses ZEB2L transcription and also takes part in posttranscriptional regulation of ZEB2L during ZEA production. Our findings reveal the intriguing mechanism that the PKA pathway regulates secondary metabolite production by reprograming alternative transcription. PMID:26907763

  12. Lambda Station: Alternate network path forwarding for production SciDAC applications

    SciTech Connect

    Grigoriev, Maxim; Bobyshev, Andrey; Crawford, Matt; DeMar, Phil; Grigaliunas, Vyto; Moibenko, Alexander; Petravick, Don; Newman, Harvey; Steenberg, Conrad; Thomas, Michael; /Caltech

    2007-09-01

    The LHC era will start very soon, creating immense data volumes capable of demanding allocation of an entire network circuit for task-driven applications. Circuit-based alternate network paths are one solution to meeting the LHC high bandwidth network requirements. The Lambda Station project is aimed at addressing growing requirements for dynamic allocation of alternate network paths. Lambda Station facilitates the rerouting of designated traffic through site LAN infrastructure onto so-called 'high-impact' wide-area networks. The prototype Lambda Station developed with Service Oriented Architecture (SOA) approach in mind will be presented. Lambda Station has been successfully integrated into the production version of the Storage Resource Manager (SRM), and deployed at US CMS Tier1 center at Fermilab, as well as at US-CMS Tier-2 site at Caltech. This paper will discuss experiences using the prototype system with production SciDAC applications for data movement between Fermilab and Caltech. The architecture and design principles of the production version Lambda Station software, currently being implemented as Java based web services, will also be presented in this paper.

  13. Solving Real World Problems with Alternate Reality Gaming: Student Experiences in the Global Village Playground Capstone Course Design

    ERIC Educational Resources Information Center

    Dondlinger, Mary Jo; McLeod, Julie K.

    2015-01-01

    The Global Village Playground (GVP) was a capstone learning experience designed to address institutional assessment needs while providing an integrated and authentic learning experience for students aimed at fostering complex problem solving, as well as critical and creative thinking. In the GVP, students work on simulated and real-world problems…

  14. MAG4 Versus Alternative Techniques for Forecasting Active-Region Flare Productivity

    NASA Technical Reports Server (NTRS)

    Falconer, David A.; Moore, Ronald L.; Barghouty, Abdulnasser F.; Khazanov, Igor

    2014-01-01

    MAG4 is a technique of forecasting an active region's rate of production of major flares in the coming few days from a free-magnetic-energy proxy. We present a statistical method of measuring the difference in performance between MAG4 and comparable alternative techniques that forecast an active region's major-flare productivity from alternative observed aspects of the active region. We demonstrate the method by measuring the difference in performance between the "Present MAG4" technique and each of three alternative techniques, called "McIntosh Active-Region Class," "Total Magnetic Flux," and "Next MAG4." We do this by using (1) the MAG4 database of magnetograms and major-flare histories of sunspot active regions, (2) the NOAA table of the major-flare productivity of each of 60 McIntosh active-region classes of sunspot active regions, and (3) five technique-performance metrics (Heidke Skill Score, True Skill Score, Percent Correct, Probability of Detection, and False Alarm Rate) evaluated from 2000 random two-by-two contingency tables obtained from the databases. We find that (1) Present MAG4 far outperforms both McIntosh Active-Region Class and Total Magnetic Flux, (2) Next MAG4 significantly outperforms Present MAG4, (3) the performance of Next MAG4 is insensitive to the forward and backward temporal windows used, in the range of one to a few days, and (4) forecasting from the free-energy proxy in combination with either any broad category of McIntosh active-region classes or any Mount Wilson active-region class gives no significant performance improvement over forecasting from the free-energy proxy alone (Present MAG4).

  15. Evaluation of the environmental performance of alternatives for polystyrene production in Brazil.

    PubMed

    Hansen, Adriana Petrella; da Silva, Gil Anderi; Kulay, Luiz

    2015-11-01

    The global demand for polystyrene is supposed to reach an overall baseline of 23.5 million tons by 2020. The market has experienced the effects of such growth, especially regarding the environmental performance of the production processes. In Brazil, renewable assets have been used to overcome the adverse consequences of this expansion. This study evaluates this issue for the production of Brazilian polystyrene resins, general-purpose polystyrene (GPPS) and high-impact polystyrene (HIPS). The effects of replacing fossil ethylene with a biobased alternative are also investigated. Life Cycle Assessment is applied for ten scenarios, with different technological approaches for renewable ethylene production and an alternative for obtaining bioethanol, which considers the export of electricity. The fossil GPPS and HIPS show a better performance than the partially renewable sources in terms of Climate Change (CC), Terrestrial Acidification (TA), Photochemical Oxidant Formation (POF), and Water Depletion (WD). The exception is Fossil Depletion (FD), a somewhat predictable result. The main environmental loads associated with the renewable options are related to the sugarcane production. Polybutadiene fails to provide greater additional impact to HIPS when compared to GPPS. With regard to obtaining ethylene from ethanol, Adiabatic Dehydration (AD) technology consumes less sugarcane than Adiabatic Dehydration at High Pressure (ADHP), which leads to gains in TA and POF. In contrast, ADHP was more eco-friendly for WD because of its lower water losses and in terms of CC because of the advantageous balance of fossil CO2(eq) at the agricultural stage and the lower consumption of natural gas in ethylene production. The electricity export is an auspicious environmental opportunity because it can counterbalance some of the negative impacts associated with the renewable route. According to a "cradle-to-grave" perspective, the partially renewable resins show a more favorable balance of

  16. Quantitative Framework to Evaluate Alternative Dispute Resolution Investments in Architecture Engineering and Construction Projects Using Option and Real Option Theory

    ERIC Educational Resources Information Center

    Menassa, Carol Chukri

    2009-01-01

    A project-specific dispute resolution ladder (DRL) typically consists of multiple alternative dispute resolution (ADR) techniques that are chosen to assist in mitigating the impact of change orders and claims (CCO) occurring during the project construction phase, and avoid their escalation to protracted disputes that adversely affect a…

  17. Applications for Near-Real Time Satellite Cloud and Radiation Products

    NASA Technical Reports Server (NTRS)

    Minnis, Patrick; Palikonda, Rabindra; Chee, Thad L.; Bedka, Kristopher M.; Smith, W.; Ayers, Jeffrey K.; Benjamin, Stanley; Chang, F.-L.; Nguyen, Louis; Norris, Peter; Rolf, Riechle; Reinecker, Michele; Shan, B.; DaSilva, Arlindo; Yost, Christopher R.

    2012-01-01

    At NASA Langley Research Center, a variety of cloud, clear-sky, and radiation products are being derived at different scales from regional to global using geostationary satellite (GEOSat) and lower Earth-orbiting (LEOSat) imager data. With growing availability, these products are becoming increasingly valuable for weather forecasting and nowcasting. These products include, but are not limited to, cloud-top and base heights, cloud water path and particle size, cloud temperature and phase, surface skin temperature and albedo, and top-of-atmosphere radiation budget. Some of these data products are currently assimilated operationally in a numerical weather prediction model. Others are used unofficially for nowcasting, while testing is underway for other applications. These applications include the use of cloud water path in an NWP model, cloud optical depth for detecting convective initiation in cirrus-filled skies, and aircraft icing condition diagnoses among others. This paper briefly describes a currently operating system that analyzes data from GEOSats around the globe (GOES, Meteosat, MTSAT, FY-2) and LEOSats (AVHRR and MODIS) and makes the products available in near-real time through a variety of media. Current potential future use of these products is discussed.

  18. Applications for Near-Real Time Satellite Cloud and Radiation Products

    NASA Astrophysics Data System (ADS)

    Minnis, P.; Palikonda, R.; Chee, T.; Bedka, K. M.; Smith, W.; Ayers, J.; Benjamin, S.; Chang, F.; Jones, T. A.; Nguyen, L.; Norris, P. M.; Reichle, R. H.; Rienecker, M. M.; da Silva, A.; Yost, C. R.

    2012-12-01

    At NASA Langley Research Center, a variety of cloud, clear-sky, and radiation products are being derived at different scales from regional to global using geostationary satellite (GEOSat) and lower Earth-orbiting (LEOSat) imager data. With growing availability, these products are becoming increasingly valuable for weather forecasting and nowcasting. These products include, but are not limited to, cloud-top and base heights, cloud water path and particle size, cloud temperature and phase, surface skin temperature and albedo, and top-of-atmosphere radiation budget. Some of these data products are currently assimilated operationally in a numerical weather prediction model. Others are used unofficially for nowcasting, while testing is underway for other applications. These applications include the use of cloud water path in an NWP model, cloud optical depth for detecting convective initiation in cirrus-filled skies, and aircraft icing condition diagnoses among others. This paper briefly describes a currently operating system that analyzes data from GEOSats around the globe (GOES, Meteosat, MTSAT, FY-2) and LEOSats (AVHRR and MODIS) and makes the products available in near-real time through a variety of media. Current potential future use of these products is discussed.

  19. Modeling of Nitrous Oxide Production from Nitritation Reactors Treating Real Anaerobic Digestion Liquor.

    PubMed

    Wang, Qilin; Ni, Bing-Jie; Lemaire, Romain; Hao, Xiaodi; Yuan, Zhiguo

    2016-01-01

    In this work, a mathematical model including both ammonium oxidizing bacteria (AOB) and heterotrophic bacteria (HB) is constructed to predict N2O production from the nitritation systems receiving the real anaerobic digestion liquor. This is for the first time that N2O production from such systems was modeled considering both AOB and HB. The model was calibrated and validated using experimental data from both lab- and pilot-scale nitritation reactors. The model predictions matched the dynamic N2O, ammonium, nitrite and chemical oxygen demand data well, supporting the capability of the model. Modeling results indicated that HB are the dominant contributor to N2O production in the above systems with the dissolved oxygen (DO) concentration of 0.5-1.0 mg O2/L, accounting for approximately 75% of N2O production. The modeling results also suggested that the contribution of HB to N2O production decreased with the increasing DO concentrations, from 75% at DO = 0.5 mg O2/L to 25% at DO = 7.0 mg O2/L, with a corresponding increase of the AOB contribution (from 25% to 75%). Similar to HB, the total N2O production rate also decreased dramatically from 0.65 to 0.25 mg N/L/h when DO concentration increased from 0.5 to 7.0 mg O2/L. PMID:27125491

  20. Modeling of Nitrous Oxide Production from Nitritation Reactors Treating Real Anaerobic Digestion Liquor

    PubMed Central

    Wang, Qilin; Ni, Bing-Jie; Lemaire, Romain; Hao, Xiaodi; Yuan, Zhiguo

    2016-01-01

    In this work, a mathematical model including both ammonium oxidizing bacteria (AOB) and heterotrophic bacteria (HB) is constructed to predict N2O production from the nitritation systems receiving the real anaerobic digestion liquor. This is for the first time that N2O production from such systems was modeled considering both AOB and HB. The model was calibrated and validated using experimental data from both lab- and pilot-scale nitritation reactors. The model predictions matched the dynamic N2O, ammonium, nitrite and chemical oxygen demand data well, supporting the capability of the model. Modeling results indicated that HB are the dominant contributor to N2O production in the above systems with the dissolved oxygen (DO) concentration of 0.5–1.0 mg O2/L, accounting for approximately 75% of N2O production. The modeling results also suggested that the contribution of HB to N2O production decreased with the increasing DO concentrations, from 75% at DO = 0.5 mg O2/L to 25% at DO = 7.0 mg O2/L, with a corresponding increase of the AOB contribution (from 25% to 75%). Similar to HB, the total N2O production rate also decreased dramatically from 0.65 to 0.25 mg N/L/h when DO concentration increased from 0.5 to 7.0 mg O2/L. PMID:27125491

  1. Near-Real-Time Satellite Cloud Products for Icing Detection and Aviation Weather over the USA

    NASA Technical Reports Server (NTRS)

    Minnis, Patrick; Smith, William L., Jr.; Nguyen, Louis; Murray, J. J.; Heck, Patrick W.; Khaiyer, Mandana M.

    2003-01-01

    A set of physically based retrieval algorithms has been developed to derive from multispectral satellite imagery a variety of cloud properties that can be used to diagnose icing conditions when upper-level clouds are absent. The algorithms are being applied in near-real time to the Geostationary Operational Environmental Satellite (GOES) data over Florida, the Southern Great Plains, and the midwestern USA. The products are available in image and digital formats on the world-wide web. The analysis system is being upgraded to analyze GOES data over the CONUS. Validation, 24-hour processing, and operational issues are discussed.

  2. New biofuel alternatives: integrating waste management and single cell oil production.

    PubMed

    Martínez, Elia Judith; Raghavan, Vijaya; González-Andrés, Fernando; Gómez, Xiomar

    2015-01-01

    Concerns about greenhouse gas emissions have increased research efforts into alternatives in bio-based processes. With regard to transport fuel, bioethanol and biodiesel are still the main biofuels used. It is expected that future production of these biofuels will be based on processes using either non-food competing biomasses, or characterised by low CO₂ emissions. Many microorganisms, such as microalgae, yeast, bacteria and fungi, have the ability to accumulate oils under special culture conditions. Microbial oils might become one of the potential feed-stocks for biodiesel production in the near future. The use of these oils is currently under extensive research in order to reduce production costs associated with the fermentation process, which is a crucial factor to increase economic feasibility. An important way to reduce processing costs is the use of wastes as carbon sources. The aim of the present review is to describe the main aspects related to the use of different oleaginous microorganisms for lipid production and their performance when using bio-wastes. The possibilities for combining hydrogen (H₂) and lipid production are also explored in an attempt for improving the economic feasibility of the process. PMID:25918941

  3. New Biofuel Alternatives: Integrating Waste Management and Single Cell Oil Production

    PubMed Central

    Martínez, Elia Judith; Raghavan, Vijaya; González-Andrés, Fernando; Gómez, Xiomar

    2015-01-01

    Concerns about greenhouse gas emissions have increased research efforts into alternatives in bio-based processes. With regard to transport fuel, bioethanol and biodiesel are still the main biofuels used. It is expected that future production of these biofuels will be based on processes using either non-food competing biomasses, or characterised by low CO2 emissions. Many microorganisms, such as microalgae, yeast, bacteria and fungi, have the ability to accumulate oils under special culture conditions. Microbial oils might become one of the potential feed-stocks for biodiesel production in the near future. The use of these oils is currently under extensive research in order to reduce production costs associated with the fermentation process, which is a crucial factor to increase economic feasibility. An important way to reduce processing costs is the use of wastes as carbon sources. The aim of the present review is to describe the main aspects related to the use of different oleaginous microorganisms for lipid production and their performance when using bio-wastes. The possibilities for combining hydrogen (H2) and lipid production are also explored in an attempt for improving the economic feasibility of the process. PMID:25918941

  4. Biotechnological production of ethanol from renewable resources by Neurospora crassa: an alternative to conventional yeast fermentations?

    PubMed

    Dogaris, Ioannis; Mamma, Diomi; Kekos, Dimitris

    2013-02-01

    Microbial production of ethanol might be a potential route to replace oil and chemical feedstocks. Bioethanol is by far the most common biofuel in use worldwide. Lignocellulosic biomass is the most promising renewable resource for fuel bioethanol production. Bioconversion of lignocellulosics to ethanol consists of four major unit operations: pretreatment, hydrolysis, fermentation, and product separation/distillation. Conventional bioethanol processes for lignocellulosics apply commercial fungal cellulase enzymes for biomass hydrolysis, followed by yeast fermentation of resulting glucose to ethanol. The fungus Neurospora crassa has been used extensively for genetic, biochemical, and molecular studies as a model organism. However, the strain's potential in biotechnological applications has not been widely investigated and discussed. The fungus N. crassa has the ability to synthesize and secrete all three enzyme types involved in cellulose hydrolysis as well as various enzymes for hemicellulose degradation. In addition, N. crassa has been reported to convert to ethanol hexose and pentose sugars, cellulose polymers, and agro-industrial residues. The combination of these characteristics makes N. crassa a promising alternative candidate for biotechnological production of ethanol from renewable resources. This review consists of an overview of the ethanol process from lignocellulosic biomass, followed by cellulases and hemicellulases production, ethanol fermentations of sugars and lignocellulosics, and industrial application potential of N. crassa. PMID:23318834

  5. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes.

    PubMed

    Gómez, Sara; López-Estepa, Miguel; Fernández, Francisco J; Suárez, Teresa; Vega, M Cristina

    2016-01-01

    Besides the most established expression hosts, several eukaryotic microorganisms and filamentous fungi have also been successfully used as platforms for the production of foreign proteins. Filamentous fungi and Dictyostelium discoideum are two prominent examples. Filamentous fungi, typically Aspergillus and Trichoderma, are usually employed for the industrial production of enzymes and secondary metabolites for food processing, pharmaceutical drugs production, and textile and paper applications, with multiple products already accepted for their commercialization. The low cost of culture medium components, high secretion capability directly to the extracellular medium, and the intrinsic ability to produce post-translational modifications similar to the mammalian type, have promoted this group as successful hosts for the expression of proteins, including examples from phylogenetically distant groups: humans proteins such as IL-2, IL-6 or epithelial growth factor; α-galactosidase from plants; or endoglucanase from Cellulomonas fimi, among others. D. discoideum is a social amoeba that can be used as an expression platform for a variety of proteins, which has been extensively illustrated for cytoskeletal proteins. New vectors for heterologous expression in D. discoideum have been recently developed that might increase the usefulness of this system and expand the range of protein classes that can be tackled. Continuous developments are ongoing to improve strains, promoters, production and downstream processes for filamentous fungi, D. discoideum, and other alternative eukaryotic hosts. Either for the overexpression of individual genes, or in the coexpression of multiples genes, this chapter illustrates the enormous possibilities offered by these groups of eukaryotic organisms. PMID:27165325

  6. 21 CFR 610.68 - Exceptions or alternatives to labeling requirements for biological products held by the Strategic...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements for biological products held by the Strategic National Stockpile. 610.68 Section 610.68 Food and... GENERAL BIOLOGICAL PRODUCTS STANDARDS Labeling Standards § 610.68 Exceptions or alternatives to labeling requirements for biological products held by the Strategic National Stockpile. (a) The appropriate FDA...

  7. 21 CFR 610.68 - Exceptions or alternatives to labeling requirements for biological products held by the Strategic...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... for biological products held by the Strategic National Stockpile. 610.68 Section 610.68 Food and Drugs... BIOLOGICAL PRODUCTS STANDARDS Labeling Standards § 610.68 Exceptions or alternatives to labeling requirements for biological products held by the Strategic National Stockpile. (a) The appropriate FDA...

  8. 21 CFR 610.68 - Exceptions or alternatives to labeling requirements for biological products held by the Strategic...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements for biological products held by the Strategic National Stockpile. 610.68 Section 610.68 Food and... GENERAL BIOLOGICAL PRODUCTS STANDARDS Labeling Standards § 610.68 Exceptions or alternatives to labeling requirements for biological products held by the Strategic National Stockpile. (a) The appropriate FDA...

  9. 21 CFR 610.68 - Exceptions or alternatives to labeling requirements for biological products held by the Strategic...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements for biological products held by the Strategic National Stockpile. 610.68 Section 610.68 Food and... GENERAL BIOLOGICAL PRODUCTS STANDARDS Labeling Standards § 610.68 Exceptions or alternatives to labeling requirements for biological products held by the Strategic National Stockpile. (a) The appropriate FDA...

  10. 21 CFR 610.68 - Exceptions or alternatives to labeling requirements for biological products held by the Strategic...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements for biological products held by the Strategic National Stockpile. 610.68 Section 610.68 Food and... GENERAL BIOLOGICAL PRODUCTS STANDARDS Labeling Standards § 610.68 Exceptions or alternatives to labeling requirements for biological products held by the Strategic National Stockpile. (a) The appropriate FDA...

  11. 21 CFR 809.11 - Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... requirements for in vitro diagnostic products for human use held by the Strategic National Stockpile. 809.11... (CONTINUED) MEDICAL DEVICES IN VITRO DIAGNOSTIC PRODUCTS FOR HUMAN USE Labeling § 809.11 Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human use held by the...

  12. 21 CFR 809.11 - Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... requirements for in vitro diagnostic products for human use held by the Strategic National Stockpile. 809.11... (CONTINUED) MEDICAL DEVICES IN VITRO DIAGNOSTIC PRODUCTS FOR HUMAN USE Labeling § 809.11 Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human use held by the...

  13. 21 CFR 809.11 - Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... requirements for in vitro diagnostic products for human use held by the Strategic National Stockpile. 809.11... (CONTINUED) MEDICAL DEVICES IN VITRO DIAGNOSTIC PRODUCTS FOR HUMAN USE Labeling § 809.11 Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human use held by the...

  14. 21 CFR 809.11 - Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... requirements for in vitro diagnostic products for human use held by the Strategic National Stockpile. 809.11... (CONTINUED) MEDICAL DEVICES IN VITRO DIAGNOSTIC PRODUCTS FOR HUMAN USE Labeling § 809.11 Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human use held by the...

  15. 40 CFR 63.5985 - What are my alternatives for meeting the emission limits for tire production affected sources?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the emission limits for tire production affected sources? 63.5985 Section 63.5985 Protection of... Hazardous Air Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Production Affected Sources § 63.5985 What are my alternatives for meeting the emission limits for tire production affected...

  16. 40 CFR 63.5985 - What are my alternatives for meeting the emission limits for tire production affected sources?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the emission limits for tire production affected sources? 63.5985 Section 63.5985 Protection of... Hazardous Air Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Production Affected Sources § 63.5985 What are my alternatives for meeting the emission limits for tire production affected...

  17. 40 CFR 63.5987 - What are my alternatives for meeting the emission limits for tire cord production affected sources?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the emission limits for tire cord production affected sources? 63.5987 Section 63.5987 Protection of... Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Cord Production Affected Sources § 63.5987 What are my alternatives for meeting the emission limits for tire cord production affected sources?...

  18. 40 CFR 63.5987 - What are my alternatives for meeting the emission limits for tire cord production affected sources?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the emission limits for tire cord production affected sources? 63.5987 Section 63.5987 Protection of... Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Cord Production Affected Sources § 63.5987 What are my alternatives for meeting the emission limits for tire cord production affected sources?...

  19. 40 CFR 63.5985 - What are my alternatives for meeting the emission limits for tire production affected sources?

    Code of Federal Regulations, 2011 CFR

    2011-07-01

    ... the emission limits for tire production affected sources? 63.5985 Section 63.5985 Protection of... Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Production Affected Sources § 63.5985 What are my alternatives for meeting the emission limits for tire production affected sources? You must...

  20. 40 CFR 63.5985 - What are my alternatives for meeting the emission limits for tire production affected sources?

    Code of Federal Regulations, 2010 CFR

    2010-07-01

    ... the emission limits for tire production affected sources? 63.5985 Section 63.5985 Protection of... Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Production Affected Sources § 63.5985 What are my alternatives for meeting the emission limits for tire production affected sources? You must...

  1. 40 CFR 63.5985 - What are my alternatives for meeting the emission limits for tire production affected sources?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the emission limits for tire production affected sources? 63.5985 Section 63.5985 Protection of... Hazardous Air Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Production Affected Sources § 63.5985 What are my alternatives for meeting the emission limits for tire production affected...

  2. Women's attitudes towards the use of complementary and alternative medicine products during pregnancy.

    PubMed

    Frawley, J; Sibbritt, D; Broom, A; Gallois, C; Steel, A; Adams, J

    2016-05-01

    The aim of this study was to analyse women's attitudes towards the use of complementary and alternative medicine (CAM) products during pregnancy. The study sample was obtained via the Australian Longitudinal Study on Women's Health or ALSWH. A response rate of 79.2% (n = 1,835) was attained. Women who use herbal medicines (34.5%, n = 588) view CAM as a preventative measure, are looking for something holistic and are concerned about evidence of clinical efficacy when considering the use of these products during pregnancy. Women who use aromatherapy (17.4%, n = 319) and homoeopathy (13.3%, n = 244) want more personal control over their body and are concerned more about their own personal experience of the efficacy of CAM than clinical evidence of efficacy. As CAM use in pregnancy appears to be increasingly commonplace, insights into women's attitudes towards CAM are valuable for maternity healthcare providers. PMID:26472482

  3. Biomarkers of Subclinical Atherosclerosis and Natural Products as Complementary Alternative Medicine.

    PubMed

    Klafke, Jonatas Zeni; Porto, Fernando Garcez; de Almeida, Amanda Spring; Parisi, Mariana Migliorini; Hirsch, Gabriela Elisa; Trevisan, Gabriela; Viecili, Paulo Ricardo Nazário

    2016-01-01

    Cardiovascular diseases (CVD) are considered the leading cause of morbidity and mortality from chronic diseases in the world. In addition, about 20% of first and recurrent acute myocardial infarctions (MI) are silent. In this context, subclinical atherosclerosis culminates in evident CVD, through the evolution of early risk factors such as hypercholesterolemia, hypertriglyceridemia and others. The main problem in CVD is related to the long-time between the start of the subclinical atherosclerosis and the manifestation of the disease. The identification of subjects at risk of such events is obviously substantial, since identification leads to implementation and compliance with effective preventive measures that reduce such risk. In this sense, this review demonstrates biomarkers as an alternative to early detection of subclinical atherosclerosis. One of the proposed biomarkers is the Ischemia-modified albumin (IMA), being considered a promising biochemical biomarker for atherosclerotic conditions. Another marker that is gaining strength and is associated with the IMA are the advanced oxidation protein products (AOPP), its measurement provides information on the level of exposure to potentially harmful changes to proteins and metabolic control. And last but not least we have nitric oxide as an early marker mainly related to endothelial dysfunction. In this review also is evidenced the use of the Campomanesia xanthocarpa, a plant native to southern region from Brazil extensively used as complementary and alternative medicine, and natural products to reduce protein oxidation and improve the availability of nitric oxide and consequently vascular function, reducing the risk for development of CVD. PMID:26561067

  4. First measurement of Z/γ* production in compton scattering of quasi-real photons

    NASA Astrophysics Data System (ADS)

    OPAL Collaboration; Abbiendi, G.; Ackerstaff, K.; Alexander, G.; Allison, J.; Altekamp, N.; Anderson, K. J.; Anderson, S.; Arcelli, S.; Asai, S.; Ashby, S. F.; Axen, D.; Azuelos, G.; Ball, A. H.; Barberio, E.; Barlow, R. J.; Bartoldus, R.; Batley, J. R.; Baumann, S.; Bechtluft, J.; Behnke, T.; Bell, K. W.; Bella, G.; Bellerive, A.; Bentvelsen, S.; Bethke, S.; Betts, S.; Biebel, O.; Biguzzi, A.; Bird, S. D.; Blobel, V.; Bloodworth, I. J.; Bobinski, M.; Bock, P.; Böhme, J.; Bonacorsi, D.; Boutemeur, M.; Braibant, S.; Bright-Thomas, P.; Brigliadori, L.; Brown, R. M.; Burckhart, H. J.; Burgard, C.; Bürgin, R.; Capiluppi, P.; Carnegie, R. K.; Carter, A. A.; Carter, J. R.; Chang, C. Y.; Charlton, D. G.; Chrisman, D.; Ciocca, C.; Clarke, P. E. L.; Clay, E.; Cohen, I.; Conboy, J. E.; Cooke, O. C.; Couyoumtzelis, C.; Coxe, R. L.; Cuffiani, M.; Dado, S.; Dallavalle, G. M.; Davis, R.; de Jong, S.; del Pozo, L. A.; de Roeck, A.; Desch, K.; Dienes, B.; Dixit, M. S.; Dubbert, J.; Duchovni, E.; Duckeck, G.; Duerdoth, I. P.; Eatough, D.; Estabrooks, P. G.; Etzion, E.; Evans, H. G.; Fabbri, F.; Fanti, M.; Faust, A. A.; Fiedler, F.; Fierro, M.; Fleck, I.; Folman, R.; Fürtjes, A.; Futyan, D. I.; Gagnon, P.; Gary, J. W.; Gascon, J.; Gascon-Shotkin, S. M.; Gaycken, G.; Geich-Gimbel, C.; Giacomelli, G.; Giacomelli, P.; Gibson, V.; Gibson, W. R.; Gingrich, D. M.; Glenzinski, D.; Goldberg, J.; Gorn, W.; Grandi, C.; Gross, E.; Grunhaus, J.; Gruwé, M.; Hanson, G. G.; Hansroul, M.; Hapke, M.; Harder, K.; Hargrove, C. K.; Hartmann, C.; Hauschild, M.; Hawkes, C. M.; Hawkings, R.; Hemingway, R. J.; Herndon, M.; Herten, G.; Heuer, R. D.; Hildreth, M. D.; Hill, J. C.; Hillier, S. J.; Hobson, P. R.; Hocker, A.; Homer, R. J.; Honma, A. K.; Horváth, D.; Hossain, K. R.; Howard, R.; Hüntemeyer, P.; Igo-Kemenes, P.; Imrie, D. C.; Ishii, K.; Jacob, F. R.; Jawahery, A.; Jeremie, H.; Jimack, M.; Jones, C. R.; Jovanovic, P.; Junk, T. R.; Karlen, D.; Kartvelishvili, V.; Kawagoe, K.; Kawamoto, T.; Kayal, P. I.; Keeler, R. K.; Kellogg, R. G.; Kennedy, B. W.; Klier, A.; Kluth, S.; Kobayashi, T.; Kobel, M.; Koetke, D. S.; Kokott, T. P.; Kolrep, M.; Komamiya, S.; Kowalewski, R. V.; Kress, T.; Krieger, P.; von Krogh, J.; Kuhl, T.; Kyberd, P.; Lafferty, G. D.; Lanske, D.; Lauber, J.; Lautenschlager, S. R.; Lawson, I.; Layter, J. G.; Lazic, D.; Lee, A. M.; Lellouch, D.; Letts, J.; Levinson, L.; Liebisch, R.; List, B.; Littlewood, C.; Lloyd, A. W.; Lloyd, S. L.; Loebinger, F. K.; Long, G. D.; Losty, M. J.; Ludwig, J.; Liu, D.; Macchiolo, A.; MacPherson, A.; Mader, W.; Mannelli, M.; Marcellini, S.; Markopoulos, C.; Martin, A. J.; Martin, J. P.; Martinez, G.; Mashimo, T.; Mättig, P.; McDonald, W. J.; McKenna, J.; McKigney, E. A.; McMahon, T. J.; McPherson, R. A.; Meijers, F.; Menke, S.; Merritt, F. S.; Mes, H.; Meyer, J.; Michelini, A.; Mihara, S.; Mikenberg, G.; Miller, D. J.; Mir, R.; Mohr, W.; Montanari, A.; Mori, T.; Nagai, K.; Nakamura, I.; Neal, H. A.; Nellen, B.; Nisius, R.; O'Neale, S. W.; Oakham, F. G.; Odorici, F.; Ogren, H. O.; Oreglia, M. J.; Orito, S.; Pálinkás, J.; Pásztor, G.; Pater, J. R.; Patrick, G. N.; Patt, J.; Perez-Ochoa, R.; Petzold, S.; Pfeifenschneider, P.; Pilcher, J. E.; Pinfold, J.; Plane, D. E.; Poffenberger, P.; Polok, J.; Przybycień , M.; Rembser, C.; Rick, H.; Robertson, S.; Robins, S. A.; Rodning, N.; Roney, J. M.; Roscoe, K.; Rossi, A. M.; Rozen, Y.; Runge, K.; Runolfsson, O.; Rust, D. R.; Sachs, K.; Saeki, T.; Sahr, O.; Sang, W. M.; Sarkisyan, E. K. G.; Sbarra, C.; Schaile, A. D.; Schaile, O.; Scharf, F.; Scharff-Hansen, P.; Schieck, J.; Schmitt, B.; Schmitt, S.; Schöning, A.; Schröder, M.; Schumacher, M.; Schwick, C.; Scott, W. G.; Seiler, T.; Seuster, R.; Shears, T. G.; Shen, B. C.; Shepherd-Themistocleous, C. H.; Sherwood, P.; Siroli, G. P.; Sittler, A.; Skuja, A.; Smith, A. M.; Snow, G. A.; Sobie, R.; Söldner-Rembold, S.; Sproston, M.; Stahl, A.; Stephens, K.; Steuerer, J.; Stoll, K.; Strom, D.; Ströhmer, R.; Surrow, B.; Talbot, S. D.; Tanaka, S.; Taras, P.; Tarem, S.; Teuscher, R.; Thiergen, M.; Thomson, M. A.; von Törne, E.; Torrence, E.; Towers, S.; Trigger, I.; Trócsányi, Z.; Tsur, E.; Turcot, A. S.; Turner-Watson, M. F.; van Kooten, R.; Vannerem, P.; Verzocchi, M.; Voss, H.; Wäckerle, F.; Wagner, A.; Ward, C. P.; Ward, D. R.; Watkins, P. M.; Watson, A. T.; Watson, N. K.; Wells, P. S.; Wermes, N.; White, J. S.; Wilson, G. W.; Wilson, J. A.; Wyatt, T. R.; Yamashita, S.; Yekutieli, G.; Zacek, V.; Zer-Zion, D.

    1998-10-01

    We report the first observation of Z/γ* production in Compton scattering of quasi-real photons. This is a subprocess of the reaction e+e--->e+e- Z/γ*, where one of the final state electrons is undetected. Approximately 55 pb-1 of data collected in the year 1997 at an e+e- centre-of-mass energy of 183 GeV with the OPAL detector at LEP have been analysed. The Z/γ* from Compton scattering has been detected in the hadronic decay channel. Within well defined kinematic bounds, we measure the product of cross-section and Z/γ* branching ratio to hadrons to be (0.9+/-0.3+/-0.1) pb for events with a hadronic mass larger than 60 GeV, dominated by (e)eZ production. In the hadronic mass region between 5 GeV and 60 GeV, dominated by (e)eγ* production, this product is found to be (4.1+/-1.6+/-0.6) pb. Our results agree with the predictions of two Monte Carlo event generators, grc4f and PYTHIA.

  5. Detection of Nicotiana DNA in Tobacco Products Using a Novel Multiplex Real-Time PCR Assay.

    PubMed

    Korchinski, Katie L; Land, Adrian D; Craft, David L; Brzezinski, Jennifer L

    2016-07-01

    Establishing that a product contains tobacco is a requirement for the U.S. Food and Drug Administration's regulation and/or prosecution of tobacco products. Therefore, a multiplex real-time PCR method was designed to determine if Nicotiana (tobacco) DNA is present in tobacco products. The PCR method simultaneously amplifies a 73 bp fragment of the cytochrome P450 monoxygenase CYP82E4 gene and 66 bp fragment in the nia-1 gene for nitrate reductase, which are detected using dual-labeled TaqMan probes. The assay is capable of detecting approximately 7.8 pg purified tobacco DNA, with a similar sensitivity for either gene target while incorporating an internal positive control (IPC). DNA was extracted from prepared tobacco products-including chewing tobacco, pipe tobacco, and snuff-or from the cut fill (no wrapper) of cigarettes and cigars. Of the 13 products analyzed, 12 were positive for both tobacco-specific markers and the IPC. DNA was also extracted from the fill of five varieties of herbal cigarettes, which were negative for both tobacco-specific gene targets and positive for the IPC. Our method expands on current assays by introducing a multiplex reaction, targeting two sequences in two different genes of interest, incorporating an IPC into the reaction, and lowering the LOD and LOQ while increasing the efficiency of the PCR. PMID:27143320

  6. Use of Real Time Satellite Infrared and Ocean Color to Produce Ocean Products

    NASA Astrophysics Data System (ADS)

    Roffer, M. A.; Muller-Karger, F. E.; Westhaver, D.; Gawlikowski, G.; Upton, M.; Hall, C.

    2014-12-01

    Real-time data products derived from infrared and ocean color satellites are useful for several types of users around the world. Highly relevant applications include recreational and commercial fisheries, commercial towing vessel and other maritime and navigation operations, and other scientific and applied marine research. Uses of the data include developing sampling strategies for research programs, tracking of water masses and ocean fronts, optimizing ship routes, evaluating water quality conditions (coastal, estuarine, oceanic), and developing fisheries and essential fish habitat indices. Important considerations for users are data access and delivery mechanisms, and data formats. At this time, the data are being generated in formats increasingly available on mobile computing platforms, and are delivered through popular interfaces including social media (Facebook, Linkedin, Twitter and others), Google Earth and other online Geographical Information Systems, or are simply distributed via subscription by email. We review 30 years of applications and describe how we develop customized products and delivery mechanisms working directly with users. We review benefits and issues of access to government databases (NOAA, NASA, ESA), standard data products, and the conversion to tailored products for our users. We discuss advantages of different product formats and of the platforms used to display and to manipulate the data.

  7. Near Real Time VIIRS EDR Products Via Direct Broadcast Using the Community Satellite Processing Package (CSPP)

    NASA Astrophysics Data System (ADS)

    Cureton, G. P.; Mindock, S.; Martin, G.; Garcia, R.; Strabala, K.; Davies, J.; Bearson, N.; Gumley, L.; Huang, A.

    2014-12-01

    The Cooperative Institute for Meteorological Satellite Studies (CIMSS) has a long history of supporting the Direct Broadcast (DB) community for various sensors, recently with the International MODIS/AIRS Processing Package (IMAPP) for the NASA EOS polar orbiters Terra and Aqua. CIMSS has continued this effort into the NPP/JPSS (previously NPOESS) era with the development of the Community Satellite Processing Package (CSPP), supporting the VIIRS, CrIS and ATMS sensors on the Suomi National Polar-orbiting Partnership (Suomi NPP) spacecraft. Within CSPP, the Visible Infrared Imaging Radiometer Suite (VIIRS) Environmental Data Record (EDR) products are based on the NOAA Operational products as implemented by Raytheon in the Algorithm Development Library (ADL), packaged for use in a Direct Broadcast environment. Examples of near real time usage of various VIIRS EDR products are given, with a focus on products obtained using CSPP (which include the Cloud Mask, Active Fires, Aerosol Optical Thickness, Sea Surface Temperature, Surface Reflectance, Vegetation Index, Surface Type and Land Surface Temperature products).

  8. Efficient and cost-reduced glucoamylase fed-batch production with alternative carbon sources.

    PubMed

    Luo, Hongzhen; Liu, Han; He, Zhenni; Zhou, Cong; Shi, Zhongping

    2015-02-01

    Glucoamylase is an important industrial enzyme. Glucoamylase production by industrial Aspergillus niger strain featured with two major problems: (i) empirical substrate feeding methods deteriorating the fermentation performance; and (ii) the high raw materials cost limiting the economics of the glucoamylase product with delegated specification. In this study, we first proposed a novel three-stage varied-rate substrate feeding strategy for efficient glucoamylase production in a 5 L bioreactor using the standard feeding medium, by comparing the changing patterns of the important physiological parameters such as DO, OUR, RQ, etc., when using different substrate feeding strategies. With this strategy, the glucoamylase activity and productivity reached higher levels of 11,000 U/ml and 84.6 U/ml/h, respectively. The performance enhancement in this case was beneficial from the following results: DO and OUR could be controlled at the higher levels (30%, 43.83 mmol/l/h), while RQ was maintained at a stable/lower level of 0.60 simultaneously throughout the fed-batch phase. Based on this three-stage varied-rate substrate feeding strategy, we further evaluated the economics of using alternative carbon sources, attempting to reduce the raw materials cost. The results revealed that cornstarch hydrolysate could be considered as the best carbon source to replace the standard and expensive feeding medium. In this case, the production cost of the glucoamylase with delegated specification (5,000 U/ml) could be saved by more than 61% while the product quality be ensured simultaneously. The proposed strategy showed application potential in improving the economics of industrial glucoamylase production. PMID:25262682

  9. Evaluation of residual antibacterial potency in antibiotic production wastewater using a real-time quantitative method.

    PubMed

    Zhang, Hong; Zhang, Yu; Yang, Min; Liu, Miaomiao

    2015-11-01

    While antibiotic pollution has attracted considerable attention due to its potential in promoting the dissemination of antibiotic resistance genes in the environment, the antibiotic activity of their related substances has been neglected, which may underestimate the environmental impacts of antibiotic wastewater discharge. In this study, a real-time quantitative approach was established to evaluate the residual antibacterial potency of antibiotics and related substances in antibiotic production wastewater (APW) by comparing the growth of a standard bacterial strain (Staphylococcus aureus) in tested water samples with a standard reference substance (e.g. oxytetracycline). Antibiotic equivalent quantity (EQ) was used to express antibacterial potency, which made it possible to assess the contribution of each compound to the antibiotic activity in APW. The real-time quantitative method showed better repeatability (Relative Standard Deviation, RSD 1.08%) compared with the conventional fixed growth time method (RSD 5.62-11.29%). And its quantification limits ranged from 0.20 to 24.00 μg L(-1), depending on the antibiotic. We applied the developed method to analyze the residual potency of water samples from four APW treatment systems, and confirmed a significant contribution from antibiotic transformation products to potent antibacterial activity. Specifically, neospiramycin, a major transformation product of spiramycin, was found to contribute 13.15-22.89% of residual potency in spiramycin production wastewater. In addition, some unknown related substances with antimicrobial activity were indicated in the effluent. This developed approach will be effective for the management of antibacterial potency discharge from antibiotic wastewater and other waste streams. PMID:26395288

  10. What is the real price of hydroelectric production on the Senegal River?

    NASA Astrophysics Data System (ADS)

    Raso, Luciano; Bader, Jean-Claude; Malaterre, Pierre-Olivier

    2014-05-01

    Manantali is an annual reservoir on the Senegal River, located in Mali and serving Senegal and Mauritania. The reservoir is used to regulate the flow for hydroelectric production, in the face of the extremely variable seasonal climate of the region. Manantali has been operative for about 10 years now, exceeding the planned production capacity. The economic benefit comes at a price. Before the dam's construction, the annual flood was the basis of flood recession agriculture, traditionally practiced by the local population. Hydroelectric production requires a more regular flow; therefore flow peaks that used to create the flood are now dumped in the reservoir. Floods are reduced because the current reservoir management privileges hydroelectric production to flood recession agriculture. Moreover, the local water authority is evaluating the construction of 6 more reservoirs, which will enhance even further the controllability of the river flow. This study assesses the externalities of energy production for the agricultural production, quantifying the reduction of flooded surface when energy production is maximized, or alternatively, the loss energy production to maintain a minimum sustainable flood. In addition, we examine the system reliability against extreme events, and how a better use of hydrological information can improve the present reservoir management, in order to find a win-win solution. In this study we employ Stochastic Dual Dynamic Programming (SDDP) methodology. SDDP is a leaner version of Stochastic Dynamic Programming (SDP). SDDP does not suffer of the "curse of dimensionality", and therefore it can be applied to larger systems. In this application we include in the model: i) A semi-distributed hydrological model, ii) the reservoir, iii) the hydraulic routing process within the catchment and from the reservoir to the floodplain.

  11. Wheat straw degradation and production of alternative substrates for nitrogenase of Rhodobacter sphaeroides.

    PubMed

    Dziga, Dariusz; Jagiełło-Flasińska, Dominika

    2015-01-01

    Cellulose is a major component of plant biomass and could be applied in the production of biofuels, especially bioethanol. An alternative approach is production of a clean fuel - hydrogen from cellulosic biomass. In this paper an innovatory model of cellulosic waste degradation has been proposed to verify the possibility of utilization of cellulose derivatives by purple non-sulfur bacteria. The concept is based on a two-step process of wheat straw conversion by bacteria in order to obtain an organic acid mixture. In the next stage such products are consumed by Rhodobacter sphaeroides, the known producer of hydrogen. It has been documented that Cellulomonas uda expresses cellulolytic activity in the presence of wheat straw as an only source of carbon. R. sphaeroides applied in this research can effectively consume organic acids released from straw by C. uda and Lactobacillus rhamnosus and is able to grow in the presence of these substrates. Additionally, an increased nitrogenase activity of R. sphaeroides has been indicated when bacteria were cultivated in the presence of cellulose derivatives which suggests that hydrogen production occurs. PMID:26192769

  12. Experimental Study of Entropy Production in Cells under Alternating Electric Field

    NASA Astrophysics Data System (ADS)

    Ding, Chang-Jiang; Luo, Liao-Fu

    2012-08-01

    We put forward a new method for measuring the entropy production in the living cell. It involves heating the sample by alternating the electric field and recording the outward heat flow. The entropy production in a normal cell MCF10A and a cancerous cell MDA-MB-231 were measured and compared. The results show that the method is effective for the entropy measurement of a living organism. The scaled electro-induced entropy production rate (SEEP) of MDA-MB-231 monotonically increases with the electric field strength at 5-40 V/cm. While that of MCF10A changes non-monotonically and there exists a peak at 5-30 V/cm. The electro-induced entropy production ratio (EEPR) is smaller than 1 in a large range of field strengths, from 5 to 25 V/cm, which reveals that under 5-25 V/cm electric field exposure, the direction of the entropy flow may be changed from normal tissue to cancerous cells. We present a facile and effective strategy for experimentally investigating the thermodynamic properties of the cell and give a deeper insight into the physical difference between normal and cancerous cells under electric field exposure.

  13. Expression of the alternative oxidase mitigates beta-amyloid production and toxicity in model systems.

    PubMed

    El-Khoury, Riyad; Kaulio, Eveliina; Lassila, Katariina A; Crowther, Damian C; Jacobs, Howard T; Rustin, Pierre

    2016-07-01

    Mitochondrial dysfunction has been widely associated with the pathology of Alzheimer's disease, but there is no consensus on whether it is a cause or consequence of disease, nor on the precise mechanism(s). We addressed these issues by testing the effects of expressing the alternative oxidase AOX from Ciona intestinalis, in different models of AD pathology. AOX can restore respiratory electron flow when the cytochrome segment of the mitochondrial respiratory chain is inhibited, supporting ATP synthesis, maintaining cellular redox homeostasis and mitigating excess superoxide production at respiratory complexes I and III. In human HEK293-derived cells, AOX expression decreased the production of beta-amyloid peptide resulting from antimycin inhibition of respiratory complex III. Because hydrogen peroxide was neither a direct product nor substrate of AOX, the ability of AOX to mimic antioxidants in this assay must be indirect. In addition, AOX expression was able to partially alleviate the short lifespan of Drosophila models neuronally expressing human beta-amyloid peptides, whilst abrogating the induction of markers of oxidative stress. Our findings support the idea of respiratory chain dysfunction and excess ROS production as both an early step and as a pathologically meaningful target in Alzheimer's disease pathogenesis, supporting the concept of a mitochondrial vicious cycle underlying the disease. PMID:27094492

  14. Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production.

    PubMed

    Yang, Wenqiang; Catalanotti, Claudia; D'Adamo, Sarah; Wittkopp, Tyler M; Ingram-Smith, Cheryl J; Mackinder, Luke; Miller, Tarryn E; Heuberger, Adam L; Peers, Graham; Smith, Kerry S; Jonikas, Martin C; Grossman, Arthur R; Posewitz, Matthew C

    2014-11-01

    Chlamydomonas reinhardtii insertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosphate acetyltransferase (EC 2.3.1.8) (PAT2, but not PAT1) were isolated to characterize fermentative acetate production. ACK1 and PAT2 were localized to chloroplasts, while ACK2 and PAT1 were shown to be in mitochondria. Characterization of the mutants showed that PAT2 and ACK1 activity in chloroplasts plays a dominant role (relative to ACK2 and PAT1 in mitochondria) in producing acetate under dark, anoxic conditions and, surprisingly, also suggested that Chlamydomonas has other pathways that generate acetate in the absence of ACK activity. We identified a number of proteins associated with alternative pathways for acetate production that are encoded on the Chlamydomonas genome. Furthermore, we observed that only modest alterations in the accumulation of fermentative products occurred in the ack1, ack2, and ack1 ack2 mutants, which contrasts with the substantial metabolite alterations described in strains devoid of other key fermentation enzymes. PMID:25381350

  15. Real-time Transmission and Distribution of NOAA Tail Doppler Radar Data and Other Data Products

    NASA Astrophysics Data System (ADS)

    Carswell, J.; Chang, P.; Robinson, D.; Gamache, J.; Hill, J.

    2011-12-01

    distribution in near real-time, include but are not limited to, the NOAA Lower Fuselage Radar reflectivity profiles, SFMR retrievals, flight level data, AXBT profiles and Imaging Wind and Rain Airborne Profiler data. The transmission and distribution of these data has a latency of only several seconds from initial acquisition on the aircraft to end users accessing the data through the Internet enabling end users to have a virtual seat on the aircraft and quick dissemination critical observations to the hurricane research, forecasting and modeling communities. In this presentation, the system capabilities and architecture will be described. Examples of the data products and data visualization tools (client applications) will be shown.

  16. Applications of Near Real-Time Image and Fire Products from MODIS

    NASA Astrophysics Data System (ADS)

    Schmaltz, J. E.; Ilavajhala, S.; Teague, M.; Ye, G.; Masuoka, E.; Davies, D.; Murphy, K. J.; Michael, K.

    2010-12-01

    NASA’s MODIS Rapid Response Project (http://rapidfire.sci.gsfc.nasa.gov/) has been providing MODIS fire detections and imagery in near real-time since 2001. The Rapid Response system is part of the Land and Atmospheres Near-real time Capability for EOS (LANCE-MODIS) system. Current capabilities include providing MODIS imagery in true color and false color band combinations, a vegetation index, and temperature - in both uncorrected swath format and geographically corrected subset regions. The geographically-corrected subsets images cover the world's land areas and adjoining waters, as well as the entire Arctic and Antarctic. These data are available within a few hours of data acquisition. The images are accessed by large number of user communities to obtain a rapid, 250 meter-resolution overview of ground conditions for fire management, crop and famine monitoring and forecasting, disaster response (fires, oil spills, floods, storms), dust and aerosol monitoring, aviation (tracking volcanic ash), monitoring sea ice conditions, environmental monitoring, and more. In addition, the scientific community uses imagery to locate phenomena of interest prior to ordering and processing data and to support the day-to-day planning of field campaigns. The MODIS Rapid Response project has also been providing a near real-time data feed on fire locations and MODIS imagery subsets to the Fire Information for Resource Management System (FIRMS) project (http://maps.geog.umd.edu/firms). FIRMS provides timely availability of fire location information, which is essential in preventing and fighting large forest/wild fires. Products are available through a WebGIS for visualizing MODIS hotspots and MCD45 Burned Area images, an email alerting tool to deliver fire data on daily/weekly/near real-time basis, active data downloads in formats such as shape, KML, CSV, WMS, etc., along with MODIS imagery subsets. FIRMS’ user base covers more than 100 countries and territories. A recent user

  17. NASA Langley Atmospheric Science Data Centers Near Real-Time Data Products

    NASA Astrophysics Data System (ADS)

    Davenport, T.; Parker, L.; Rinsland, P. L.

    2014-12-01

    Over the past decade the Atmospheric Science Data Center (ASDC) at NASA Langley Research Center has archived and distributed a variety of satellite mission data sets. NASA's goal in Earth science is to observe, understand, and model the Earth system to discover how it is changing, to better predict change, and to understand the consequences for life on Earth. The ASDC has collaborated with Science Teams to accommodate emerging science users in the climate and modeling communities. The ASDC has expanded its original role to support operational usage by related Earth Science satellites, support land and ocean assimilations, support of field campaigns, outreach programs, and application projects for agriculture and energy industries to bridge the gap between Earth science research results and the adoption of data and prediction capabilities for reliable and sustained use in Decision Support Systems (DSS). For example; these products are being used by the community performing data assimilations to regulate aerosol mass in global transport models to improve model response and forecast accuracy, to assess the performance of components of a global coupled atmospheric-ocean climate model, improve atmospheric motion vector (winds) impact on numerical weather prediction models, and to provide internet-based access to parameters specifically tailored to assist in the design of solar and wind powered renewable energy systems. These more focused applications often require Near Real-Time (NRT) products. Generating NRT products pose their own unique set challenges for the ASDC and the Science Teams. Examples of ASDC NRT products and challenges will be discussed.

  18. MAG4 versus Alternative Techniques for Forecasting Active-Region Flare Productivity

    NASA Astrophysics Data System (ADS)

    Falconer, David; Moore, Ronald L.; Barghouty, Abdulnasser F; Khazanov, Igor

    2014-06-01

    MAG4 is a technique of forecasting an active region's rate of production of major flares in the coming few days from a free-magnetic-energy proxy. We present a statistical method of measuring the difference in performance between MAG4 and comparable alternative techniques that forecast an active region’s major-flare productivity from alternative observed aspects of the active region. We demonstrate the method by measuring the difference in performance between the “Present MAG4” technique and each of three alternative techniques, called “McIntosh Active-Region Class,” “Total Magnetic Flux,” and “Next MAG4.” We do this by using (1) the MAG4 database of magnetograms and major-flare histories of sunspot active regions, (2) the NOAA table of the major-flare productivity of each of 60 McIntosh active-region classes of sunspot active regions, and (3) five technique-performance metrics (Heidke Skill Score, True Skill Score, Percent Correct, Probability of Detection, and False Alarm Rate) evaluated from 2000 random two-by-two contingency tables obtained from the databases. We find that (1) Present MAG4 far outperforms both McIntosh Active-Region Class and Total Magnetic Flux, (2) Next MAG4 significantly outperforms Present MAG4, (3) the performance of Next MAG4 is insensitive to the forward and backward temporal windows used, in the range of one to a few days, and (4) forecasting from the free-energy proxy in combination with either any broad category of McIntosh active-region classes or any Mount Wilson active-region class gives no significant performance improvement over forecasting from the free-energy proxy alone (Present MAG4). Funding for this research came from NASA’s Game Changing Development Program, Johnson Space Center’s Space Radiation Analysis Group (SRAG), and AFOSR’s Multi-University Research Initiative. In particular, funding was facilitated by Dr. Dan Fry (NASA-JSC) and David Moore (NASA-LaRC).

  19. MAG4 versus alternative techniques for forecasting active region flare productivity

    PubMed Central

    Falconer, David A; Moore, Ronald L; Barghouty, Abdulnasser F; Khazanov, Igor

    2014-01-01

    MAG4 is a technique of forecasting an active region's rate of production of major flares in the coming few days from a free magnetic energy proxy. We present a statistical method of measuring the difference in performance between MAG4 and comparable alternative techniques that forecast an active region's major-flare productivity from alternative observed aspects of the active region. We demonstrate the method by measuring the difference in performance between the “Present MAG4” technique and each of three alternative techniques, called “McIntosh Active-Region Class,” “Total Magnetic Flux,” and “Next MAG4.” We do this by using (1) the MAG4 database of magnetograms and major flare histories of sunspot active regions, (2) the NOAA table of the major-flare productivity of each of 60 McIntosh active-region classes of sunspot active regions, and (3) five technique performance metrics (Heidke Skill Score, True Skill Score, Percent Correct, Probability of Detection, and False Alarm Rate) evaluated from 2000 random two-by-two contingency tables obtained from the databases. We find that (1) Present MAG4 far outperforms both McIntosh Active-Region Class and Total Magnetic Flux, (2) Next MAG4 significantly outperforms Present MAG4, (3) the performance of Next MAG4 is insensitive to the forward and backward temporal windows used, in the range of one to a few days, and (4) forecasting from the free-energy proxy in combination with either any broad category of McIntosh active-region classes or any Mount Wilson active-region class gives no significant performance improvement over forecasting from the free-energy proxy alone (Present MAG4). Key Points Quantitative comparison of performance of pairs of forecasting techniques Next MAG4 forecasts major flares more accurately than Present MAG4 Present MAG4 forecast outperforms McIntosh AR Class and total magnetic flux PMID:26213517

  20. Evaluation of Alternative Methods for Testing the Bioefficacy of Household Ambient Insecticide Products Against Aedes albopictus.

    PubMed

    Cassini, Rudi; Scremin, Mara; Contiero, Barbara; Drago, Andrea; Vettorato, Christian; Marcer, Federica; di Regalbono, Antonio Frangipane

    2016-06-01

    Ambient insecticides are receiving increasing attention in many developed countries because of their value in reducing mosquito nuisance. As required by the European Union Biocidal Products Regulation 528/2012, these devices require appropriate testing of their efficacy, which is based on estimating the knockdown and mortality rates of free-flying (free) mosquitoes in a test room. However, evaluations using free mosquitoes present many complexities. The performances of 6 alternative methods with mosquitoes held in 2 different cage designs (steel wire and gauze/plastic) with and without an operating fan for air circulation were monitored in a test room through a closed-circuit television system and were compared with the currently recommended method using free mosquitoes. Results for caged mosquitoes without a fan showed a clearly delayed knockdown effect, whereas outcomes for caged mosquitoes with a fan recorded higher mortality at 24 h, compared to free mosquitoes. Among the 6 methods, cages made of gauze and plastic operating with fan wind speed at 2.5-2.8 m/sec was the only method without a significant difference in results for free mosquitoes, and therefore appears as the best alternative to assess knockdown by ambient insecticides accurately. PMID:27280352

  1. Technological Alternatives or Use of Wood Fuel in Combined Heat and Power Production

    NASA Astrophysics Data System (ADS)

    Rusanova, Jekaterina; Markova, Darja; Bazbauers, Gatis; Valters, Kārlis

    2013-12-01

    Abstract Latvia aims for 40% share of renewable energy in the total final energy use. Latvia has large resources of biomass and developed district heating systems. Therefore, use of biomass for heat and power production is an economically attractive path for increase of the share of renewable energy. The optimum technological solution for use of biomass and required fuel resources have to be identified for energy planning and policy purposes. The aim of this study was to compare several wood fuel based energy conversion technologies from the technical and economical point of view. Three biomass conversion technologies for combined heat and electricity production (CHP) were analyzed: • CHP with steam turbine technology; • gasification CHP using gas engine; • bio-methane combined cycle CHP. Electricity prices for each alternative are presented. The results show the level of support needed for the analyzed renewable energy technologies and time period needed to reach price parity with the natural gas - fired combined cycle gas turbine (CCGT) CHPss. The results also show that bio-methane technology is most competitive when compared with CCGT among the considered technologies regarding fuel consumption and electricity production, but it is necessary to reduce investment costs to reach the electricity price parity with the natural gas CCGT.

  2. Membrane technology as a promising alternative in biodiesel production: a review.

    PubMed

    Shuit, Siew Hoong; Ong, Yit Thai; Lee, Keat Teong; Subhash, Bhatia; Tan, Soon Huat

    2012-01-01

    In recent years, environmental problems caused by the use of fossil fuels and the depletion of petroleum reserves have driven the world to adopt biodiesel as an alternative energy source to replace conventional petroleum-derived fuels because of biodiesel's clean and renewable nature. Biodiesel is conventionally produced in homogeneous, heterogeneous, and enzymatic catalysed processes, as well as by supercritical technology. All of these processes have their own limitations, such as wastewater generation and high energy consumption. In this context, the membrane reactor appears to be the perfect candidate to produce biodiesel because of its ability to overcome the limitations encountered by conventional production methods. Thus, the aim of this paper is to review the production of biodiesel with a membrane reactor by examining the fundamental concepts of the membrane reactor, its operating principles and the combination of membrane and catalyst in the catalytic membrane. In addition, the potential of functionalised carbon nanotubes to serve as catalysts while being incorporated into the membrane for transesterification is discussed. Furthermore, this paper will also discuss the effects of process parameters for transesterification in a membrane reactor and the advantages offered by membrane reactors for biodiesel production. This discussion is followed by some limitations faced in membrane technology. Nevertheless, based on the findings presented in this review, it is clear that the membrane reactor has the potential to be a breakthrough technology for the biodiesel industry. PMID:22366515

  3. Performance of ROB's near real-time ionospheric product during normal and disturbed space weather periods.

    NASA Astrophysics Data System (ADS)

    Bergeot, Nicolas; Chevalier, Jean-Marie; Bruyninx, Carine

    2015-04-01

    Several agencies are routinely monitoring the vertical Total Electron Content (vTEC) using GNSS data. Derived maps are available with different latencies, area extents, and grid/time resolutions. However, no high-resolution maps are publically available over Europe in near real-time. In this frame, the Royal Observatory of Belgium (ROB) developed the ROB-IONO software which takes advantage of the dense EUREF Permanent GNSS Network (EPN) to monitor the ionosphere. The main ROB products consist of ionospheric vTEC maps over Europe and their variability estimated in near real-time every 15 min on 0.5° x 0.5° grids using GPS observations. The maps are available online with a latency of ~3 min in the IONEX format at ftp://gnss.oma.be and as interactive web pages at www.gnss.be. During normal ionospheric activity, the ROB-TEC maps show a good agreement with widely used post-processed global products from IGS, CODE and ESA, with mean differences of 1.3 ± 0.9, 0.6 ± 0.7 and 0.4 ± 1.6 TECu respectively for the period 2012 to mid-2013. For a disturbed period, such as the 2003 Halloween ionospheric storm, the mean differences with IGS, CODE and ESA maps are respectively 0.9 ± 2.2, 0.1 ± 2.0 and 0.6 ± 6.8 TECu, with maximum differences (>38 TECu) occurring during the major phase of the storm. These differences are due to the lower resolution of global products in time and space compared to the ROB-TEC maps. A description of two recent events, on March 17, 2013 and February 27, 2014 highlights the capability of the method adopted to detect in near real-time abnormal ionospheric behaviour over Europe. The potential of the variability maps as an indicator of rapid ionospheric variations during the 15 min of observations is also highlighted. More than 30 ionospheric events associated with Space weather were detected during the period 2012-2014. The ionospheric perturbations are associated with Coronal Mass Ejections (CMEs, ~70% of the time), active geomagnetic conditions

  4. Fuel cells are a commercially viable alternative for the production of "clean" energy.

    PubMed

    Niakolas, Dimitris K; Daletou, Maria; Neophytides, Stylianos G; Vayenas, Constantinos G

    2016-01-01

    Fuel cells present a highly efficient and environmentally friendly alternative technology for decentralized energy production. The scope of the present study is to provide an overview of the technological and commercialization readiness level of fuel cells. Specifically, there is a brief description of their general advantages and weaknesses in correlation with various technological actions and political strategies, which are adopted towards their proper positioning in the global market. Some of the most important key performance indicators are also discussed, alongside with a few examples of broad commercialization. It is concluded that the increasing number of companies which utilize and invest on this technology, in combination with the supply chain improvements and the concomitant technological maturity and recognition, reinforce the fuel cell industry so as to become well-aligned for global success. PMID:26667058

  5. Soft Expansion of Double-Real-Virtual Corrections to Higgs Production at N$^3$LO

    DOE PAGESBeta

    Anastasiou, Charalampos; Duhr, Claude; Dulat, Falko; Furlan, Elisabetta; Herzog, Franz; Mistlberger, Bernhard

    2015-05-15

    We present methods to compute higher orders in the threshold expansion for the one-loop production of a Higgs boson in association with two partons at hadron colliders. This process contributes to the N3LO Higgs production cross section beyond the soft-virtual approximation. We use reverse unitarity to expand the phase-space integrals in the small kinematic parameters and to reduce the coefficients of the expansion to a small set of master integrals. We describe two methods for the calculation of the master integrals. The first was introduced for the calculation of the soft triple-real radiation relevant to N3LO Higgs production. The secondmore » uses a particular factorization of the three body phase-space measure and the knowledge of the scaling properties of the integral itself. Our result is presented as a Laurent expansion in the dimensional regulator, although some of the master integrals are computed to all orders in this parameter.« less

  6. Soft Expansion of Double-Real-Virtual Corrections to Higgs Production at N$^3$LO

    SciTech Connect

    Anastasiou, Charalampos; Duhr, Claude; Dulat, Falko; Furlan, Elisabetta; Herzog, Franz; Mistlberger, Bernhard

    2015-05-15

    We present methods to compute higher orders in the threshold expansion for the one-loop production of a Higgs boson in association with two partons at hadron colliders. This process contributes to the N3LO Higgs production cross section beyond the soft-virtual approximation. We use reverse unitarity to expand the phase-space integrals in the small kinematic parameters and to reduce the coefficients of the expansion to a small set of master integrals. We describe two methods for the calculation of the master integrals. The first was introduced for the calculation of the soft triple-real radiation relevant to N3LO Higgs production. The second uses a particular factorization of the three body phase-space measure and the knowledge of the scaling properties of the integral itself. Our result is presented as a Laurent expansion in the dimensional regulator, although some of the master integrals are computed to all orders in this parameter.

  7. Real-time magnetic resonance imaging and electromagnetic articulography database for speech production research (TC)

    PubMed Central

    Narayanan, Shrikanth; Toutios, Asterios; Ramanarayanan, Vikram; Lammert, Adam; Kim, Jangwon; Lee, Sungbok; Nayak, Krishna; Kim, Yoon-Chul; Zhu, Yinghua; Goldstein, Louis; Byrd, Dani; Bresch, Erik; Ghosh, Prasanta; Katsamanis, Athanasios; Proctor, Michael

    2014-01-01

    USC-TIMIT is an extensive database of multimodal speech production data, developed to complement existing resources available to the speech research community and with the intention of being continuously refined and augmented. The database currently includes real-time magnetic resonance imaging data from five male and five female speakers of American English. Electromagnetic articulography data have also been presently collected from four of these speakers. The two modalities were recorded in two independent sessions while the subjects produced the same 460 sentence corpus used previously in the MOCHA-TIMIT database. In both cases the audio signal was recorded and synchronized with the articulatory data. The database and companion software are freely available to the research community. PMID:25190403

  8. Development of a real-time bioprocess monitoring method for docosahexaenoic acid production by Schizochytrium sp.

    PubMed

    Guo, Dong-Sheng; Ji, Xiao-Jun; Ren, Lu-Jing; Li, Gan-Lu; Yin, Feng-Wei; Huang, He

    2016-09-01

    Oxygen uptake rate (OUR) and respiratory quotient (RQ) are key respiratory parameters for docosahexaenoic acid (DHA) production by Schizochytrium sp. HX-308 under dissolved oxygen limited conditions. To investigate the relationship of OUR and RQ with culture status, three independent cultures with different aeration rates were performed in a 50L bioreactor. OUR was found to be positively correlated with the aeration rate, which reflected the oxygen supply level in each culture. The highest biomass, reaching 124.5g/L, was achieved under the highest OUR. DHA content was found to be highly correlated with the RQ value, and the highest DHA content (44.85% in total fatty acids, w/w) was achieved in the highest RQ level, which implies that the polyketide synthase pathway was more active. OUR and RQ, which reflect the physiological state of microorganisms, are suggested as synergistic real-time bioprocess monitoring parameters for DHA fermentation. PMID:27262097

  9. Real-time magnetic resonance imaging and electromagnetic articulography database for speech production research (TC).

    PubMed

    Narayanan, Shrikanth; Toutios, Asterios; Ramanarayanan, Vikram; Lammert, Adam; Kim, Jangwon; Lee, Sungbok; Nayak, Krishna; Kim, Yoon-Chul; Zhu, Yinghua; Goldstein, Louis; Byrd, Dani; Bresch, Erik; Ghosh, Prasanta; Katsamanis, Athanasios; Proctor, Michael

    2014-09-01

    USC-TIMIT is an extensive database of multimodal speech production data, developed to complement existing resources available to the speech research community and with the intention of being continuously refined and augmented. The database currently includes real-time magnetic resonance imaging data from five male and five female speakers of American English. Electromagnetic articulography data have also been presently collected from four of these speakers. The two modalities were recorded in two independent sessions while the subjects produced the same 460 sentence corpus used previously in the MOCHA-TIMIT database. In both cases the audio signal was recorded and synchronized with the articulatory data. The database and companion software are freely available to the research community. PMID:25190403

  10. A New Model for Real-Time Regional Vertical Total Electron Content and Differential Code Bias Estimation Using IGS Real-Time Service (IGS-RTS) Products

    NASA Astrophysics Data System (ADS)

    Abdelazeem, Mohamed; Çelik, Rahmi N.; El-Rabbany, Ahmed

    2016-04-01

    The international global navigation satellite system (GNSS) real-time service (IGS-RTS) products have been used extensively for real-time precise point positioning and ionosphere modeling applications. In this study, we develop a regional model for real-time vertical total electron content (RT-VTEC) and differential code bias (RT-DCB) estimation over Europe using the IGS-RTS satellite orbit and clock products. The developed model has a spatial and temporal resolution of 1°×1° and 15 minutes, respectively. GPS observations from a regional network consisting of 60 IGS and EUREF reference stations are processed in the zero-difference mode using the Bernese-5.2 software package in order to extract the geometry-free linear combination of the smoothed code observations. The spherical harmonic expansion function is used to model the VTEC, the receiver and the satellite DCBs. To validate the proposed model, the RT-VTEC values are computed and compared with the final IGS-global ionospheric map (IGS-GIM) counterparts in three successive days under high solar activity including one of an extreme geomagnetic activity. The real-time satellite DCBs are also estimated and compared with the IGS-GIM counterparts. Moreover, the real-time receiver DCB for six IGS stations are obtained and compared with the IGS-GIM counterparts. The examined stations are located in different latitudes with different receiver types. The findings reveal that the estimated RT-VTEC values show agreement with the IGS-GIM counterparts with root mean-square-errors (RMSEs) values less than 2 TEC units. In addition, RMSEs of both the satellites and receivers DCBs are less than 0.85 ns and 0.65 ns, respectively in comparison with the IGS-GIM.

  11. 40 CFR 63.5987 - What are my alternatives for meeting the emission limits for tire cord production affected sources?

    Code of Federal Regulations, 2014 CFR

    2014-07-01

    ... the emission limits for tire cord production affected sources? 63.5987 Section 63.5987 Protection of... Hazardous Air Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Cord Production Affected Sources § 63.5987 What are my alternatives for meeting the emission limits for tire cord...

  12. 40 CFR 63.5987 - What are my alternatives for meeting the emission limits for tire cord production affected sources?

    Code of Federal Regulations, 2013 CFR

    2013-07-01

    ... the emission limits for tire cord production affected sources? 63.5987 Section 63.5987 Protection of... Hazardous Air Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Cord Production Affected Sources § 63.5987 What are my alternatives for meeting the emission limits for tire cord...

  13. 40 CFR 63.5987 - What are my alternatives for meeting the emission limits for tire cord production affected sources?

    Code of Federal Regulations, 2012 CFR

    2012-07-01

    ... the emission limits for tire cord production affected sources? 63.5987 Section 63.5987 Protection of... Hazardous Air Pollutants: Rubber Tire Manufacturing Emission Limits for Tire Cord Production Affected Sources § 63.5987 What are my alternatives for meeting the emission limits for tire cord...

  14. Linking carbon stock change from land-use change to consumption of agricultural products: Alternative perspectives.

    PubMed

    Goh, Chun Sheng; Wicke, Birka; Faaij, André; Bird, David Neil; Schwaiger, Hannes; Junginger, Martin

    2016-11-01

    Agricultural expansion driven by growing demand has been a key driver for carbon stock change as a consequence of land-use change (CSC-LUC). However, its relative role compared to non-agricultural and non-productive drivers, as well as propagating effects were not clearly addressed. This study contributed to this subject by providing alternative perspectives in addressing these missing links. A method was developed to allocate historical CSC-LUC to agricultural expansions by land classes (products), trade, and end use. The analysis for 1995-2010 leads to three key trends: (i) agricultural land degradation and abandonment is found to be a major (albeit indirect) driver for CSC-LUC, (ii) CSC-LUC is spurred by the growth of cross-border trade, (iii) non-food use (excluding liquid biofuels) has emerged as a significant contributor of CSC-LUC in the 2000's. In addition, the study demonstrated that exact values of CSC-LUC at a single spatio-temporal point may change significantly with different methodological settings. For example, CSC-LUC allocated to 'permanent oil crops' changed from 0.53 Pg C (billion tonne C) of carbon stock gain to 0.11 Pg C of carbon stock loss when spatial boundaries were changed from global to regional. Instead of comparing exact values for accounting purpose, key messages for policymaking were drawn from the main trends. Firstly, climate change mitigation efforts pursued through a territorial perspective may ignore indirect effects elsewhere triggered through trade linkages. Policies targeting specific commodities or types of consumption are also unable to quantitatively address indirect CSC-LUC effects because the quantification changes with different arbitrary methodological settings. Instead, it is recommended that mobilising non-productive or under-utilised lands for productive use should be targeted as a key solution to avoid direct and indirect CSC-LUC. PMID:27543749

  15. Impacts of biofuels production alternatives on water quantity and quality in the Iowa River Basin

    USGS Publications Warehouse

    Wu, Y.; Liu, S.

    2012-01-01

    Corn stover as well as perennial grasses like switchgrass (Panicum virgatum) and miscanthus are being considered as candidates for the second generation biofuel feedstocks. However, the challenges to biofuel development are its effects on the environment, especially water quality. This study evaluates the long-term impacts of biofuel production alternatives (e.g., elevated corn stover removal rates and the potential land cover change) on an ecosystem with a focus on biomass production, soil erosion, water quantity and quality, and soil nitrate nitrogen concentration at the watershed scale. The Soil and Water Assessment Tool (SWAT) was modified for setting land cover change scenarios and applied to the Iowa River Basin (a tributary of the Upper Mississippi River Basin). Results show that biomass production can be sustained with an increased stover removal rate as long as the crop demand for nutrients is met with appropriate fertilization. Although a drastic increase (4.7–70.6%) in sediment yield due to erosion and a slight decrease (1.2–3.2%) in water yield were estimated with the stover removal rate ranging between 40% and 100%, the nitrate nitrogen load declined about 6–10.1%. In comparison to growing corn, growing either switchgrass or miscanthus can reduce sediment erosion greatly. However, land cover changes from native grass to switchgrass or miscanthus would lead to a decrease in water yield and an increase in nitrate nitrogen load. In contrast to growing switchgrass, growing miscanthus is more productive in generating biomass, but its higher water demand may reduce water availability in the study area.

  16. Near-real-time global biomass burning emissions product from geostationary satellite constellation

    NASA Astrophysics Data System (ADS)

    Zhang, Xiaoyang; Kondragunta, Shobha; Ram, Jessica; Schmidt, Christopher; Huang, Ho-Chun

    2012-07-01

    Near-real-time estimates of biomass burning emissions are crucial for air quality monitoring and forecasting. We present here the first near-real-time global biomass burning emission product from geostationary satellites (GBBEP-Geo) produced from satellite-derived fire radiative power (FRP) for individual fire pixels. Specifically, the FRP is retrieved using WF_ABBA V65 (wildfire automated biomass burning algorithm) from a network of multiple geostationary satellites. The network consists of two Geostationary Operational Environmental Satellites (GOES) which are operated by the National Oceanic and Atmospheric Administration, the Meteosat second-generation satellites (Meteosat-09) operated by the European Organisation for the Exploitation of Meteorological Satellites, and the Multifunctional Transport Satellite (MTSAT) operated by the Japan Meteorological Agency. These satellites observe wildfires at an interval of 15-30 min. Because of the impacts from sensor saturation, cloud cover, and background surface, the FRP values are generally not continuously observed. The missing observations are simulated by combining the available instantaneous FRP observations within a day and a set of representative climatological diurnal patterns of FRP for various ecosystems. Finally, the simulated diurnal variation in FRP is applied to quantify biomass combustion and emissions in individual fire pixels with a latency of 1 day. By analyzing global patterns in hourly biomass burning emissions in 2010, we find that peak fire season varied greatly and that annual wildfires burned 1.33 × 1012 kg dry mass, released 1.27 × 1010 kg of PM2.5 (particulate mass for particles with diameter <2.5 μm) and 1.18 × 1011kg of CO globally (excluding most parts of boreal Asia, the Middle East, and India because of no coverage from geostationary satellites). The biomass burning emissions were mostly released from forest and savanna fires in Africa, South America, and North America. Evaluation of

  17. Sago pith residue as an alternative cheap substrate for fermentable sugars production.

    PubMed

    Linggang, S; Phang, L Y; Wasoh, M H; Abd-Aziz, S

    2012-05-01

    Sago pith residue is one of the most abundant lignocellulosic biomass which can serve as an alternative cheap substrate for fermentable sugars production. This residue is the fibrous waste left behind after the starch extraction process and contains significant amounts of starch (58%), cellulose (23%), hemicellulose (9.2%) and lignin (3.9%). The conversion of sago pith residue into fermentable sugars is commonly performed using cellulolytic enzymes or known as cellulases. In this study, crude cellulases were produced by two local isolates, Trichoderma asperellum UPM1 and Aspergillus fumigatus, UPM2 using sago pith residue as substrate. A. fumigatus UPM2 gave the highest FPase, CMCase and β-glucosidase activities of 0.39, 23.99 and 0.78 U/ml, respectively, on day 5. The highest activity of FPase, CMCase and β-glucosidase by T. asperellum UPM1 was 0.27, 12.03 and 0.42 U/ml, respectively, on day 7. The crude enzyme obtained from A. fumigatus UPM2 using β-glucosidase as the rate-limiting enzyme (3.9, 11.7 and 23.4 IU) was used for the saccharification process to convert 5% (w/v) sago pith residue into reducing sugars. Hydrolysis of sago pith residue using crude enzyme containing β-glucosidase with 23.4 IU, produced by A. fumigatus UPM2 gave higher reducing sugars production of 20.77 g/l with overall hydrolysis percentage of 73%. PMID:22528646

  18. Alternative Land-Use Scenarios for Bioenergy Production in the U.S. and Brazil

    NASA Astrophysics Data System (ADS)

    Campbell, J. E.; Spak, S.; Tsao, C. C.; Mena, M.; Chen, Y.

    2015-12-01

    Agriculture is historically a dominant form of global environmental degradation, and the potential for increased future degradation may be enhanced by growing demand for biofuels. Here, we apply high-resolution cropland inventories and agronomic models to characterize land-use impacts and mitigation scenarios for bioenergy production in the U.S. and Brazil. In the U.S., our gridded historical cropland maps show potential for production in the U.S. on 68 Mha of abandoned croplands in the U.S. which is as much as 70% larger than previous estimates due to a reduction in aggregation effects. In Brazil, a critical land-use impact is associated with non-GHG air pollutants from the management and expansion of sugarcane feedstocks. Our bottom-up estimate for these Brazilian land-use emissions is seven times larger than estimated from remote-sensing data due to the improved spatial resolution of our approach. While current land-use policies in Brazil and the U.S. seek to reduce life-cycle biofuel emissions, these policies may not support the mitigation alternatives identified here.

  19. Hazard assessment of nitrosamine and nitramine by-products of amine-based CCS: alternative approaches.

    PubMed

    Buist, H E; Devito, S; Goldbohm, R A; Stierum, R H; Venhorst, J; Kroese, E D

    2015-04-01

    Carbon capture and storage (CCS) technologies are considered vital and economic elements for achieving global CO2 reduction targets, and is currently introduced worldwide (for more information on CCS, consult for example the websites of the International Energy Agency (http://www.iea.org/topics/ccs/) and the Global CCS Institute (http://www.globalccsinstitute.com/)). One prominent CCS technology, the amine-based post-combustion process, may generate nitrosamines and their related nitramines as by-products, the former well known for their potential mutagenic and carcinogenic properties. In order to efficiently assess the carcinogenic potency of any of these by-products this paper reviews and discusses novel prediction approaches consuming less time, money and animals than the traditionally applied 2-year rodent assay. For this, available animal carcinogenicity studies with N-nitroso compounds and nitramines have been used to derive carcinogenic potency values, that were subsequently used to assess the predictive performance of alternative prediction approaches for these chemicals. Promising cancer prediction models are the QSARs developed by the Helguera group, in vitro transformation assays, and the in vivo initiation-promotion, and transgenic animal assays. All these models, however, have not been adequately explored for this purpose, as the number of N-nitroso compounds investigated is yet too limited, and therefore further testing with relevant N-nitroso compounds is needed. PMID:25604881

  20. Optimization of nisin production by Lactococcus lactis UQ2 using supplemented whey as alternative culture medium.

    PubMed

    González-Toledo, S Y; Domínguez-Domínguez, J; García-Almendárez, B E; Prado-Barragán, L A; Regalado-González, C

    2010-08-01

    Lactococcus lactis UQ2 is a nisin A-producing native strain. In the present study, the production of nisin by L. lactis UQ2 in a bioreactor using supplemented sweet whey (SW) was optimized by a statistical design of experiments and response surface methodology (RSM). In a 1st approach, a fractional factorial design (FFD) of the order 2(5-1) with 3 central points was used. The effect on nisin production of air flow, SW, soybean peptone (SP), MgSO(4)/MnSO(4) mixture, and Tween 80 was evaluated. From FFD, the most significant factors affecting nisin production were SP (P = 0.011), and SW (P = 0.037). To find optimum conditions, a central composite design (CCD) with 2 central points was used. Three factors were considered, SW (7 to 10 g/L), SP (7 to10 g/L), and small amounts of added nisin as self-inducer (NI 34.4 to 74.4 IU/L). Nisin production was expressed as international units (IU). From RSM, an optimum nisin activity of 180 IU/mL was predicted at 74.4 IU/L NI, 13.8 g/L SP, and 14.9 or 5.11 g/L SW, while confirmatory experiments showed a maximum activity of 178 +/- 5.2 IU/mL, verifying the validity of the model. The 2nd-order model showed a coefficient of determination (R(2)) of 0.828. Optimized conditions were used for constant pH fermentations, where a maximum activity of 575 +/- 17 IU/mL was achieved at pH 6.5 after 12 h. The adsorption-desorption technique was used to partially purify nisin, followed by drying. The resulting powder showed an activity of 102150 IU/g. Practical Application: Nisin production was optimized using supplemented whey as alternative culture medium, using a native L. lactis UQ2 strain. Soybean peptone, SW, and subinhibitory amounts of nisin were successfully employed to optimize nisin production by L. lactis UQ2. Dried semipurified nisin showed an activity of 102150 IU/g. PMID:20722935

  1. Characterization of odor emission from alternating aerobic and anoxic activated sludge systems using real-time total reduced sulfur analyzer.

    PubMed

    Kim, Hyunook; Lee, Hyunjoo; Choi, Eunsun; Choi, Il; Shin, Taesub; Im, Hyungjoon; Ahn, Soobin

    2014-12-01

    Anaerobic biodegradation of sulfur-containing compounds always generates volatile sulfur compounds (VSCs) including H2S, methyl mercaptan, and dimethyl sulfide (DMS). VSC emissions from wastewater treatment plants (WWTPs) result in odor complaints from people living nearby. To control odor-causing compounds in WWTPs, it is important to know the odor emission quantity particularly with continuous monitoring. Since modified activated sludge processes always include anaerobic, anoxic and aerobic conditions for nutrient removal, odor emission from these different environmental settings is expected. In this study, continuous monitoring of VSCs from the headspace of an alternating aerobic and anoxic (AAA) activated sludge process via total reduced sulfur (TRS) analyzer was performed. There is clear pattern of the initial TRS peak immediately after the initiation of the aeration in the AAA system and TRS concentration begins to drop through the remaining air-on cycle. On the other hand, during the air-off period, TRS concentrations increase with time. In particular, a clear inflection point in the TRS profile could be observed after complete removal of nitrate during air-off, meaning more VSCs formation. Since the highest odor emission occurs after the initiation of aeration, the future control of exhausted air should only deal with air collected during the initial aeration period (e.g., 30min), a similar concept for the treatment of first flush in combined sewer overflow. In addition, application of a control scheme to initiate aeration immediately after denitrification is completed during air-off should be beneficial in reducing odor emission. PMID:25180483

  2. Alternative scenarios of bioenergy crop production in an agricultural landscape and implications for bird communities.

    PubMed

    Blank, Peter J; Williams, Carol L; Sample, David W; Meehan, Timothy D; Turner, Monica G

    2016-01-01

    Increased demand and government mandates for bioenergy crops in the United States could require a large allocation of agricultural land to bioenergy feedstock production and substantially alter current landscape patterns. Incorporating bioenergy landscape design into land-use decision making could help maximize benefits and minimize trade-offs among alternative land uses. We developed spatially explicit landscape scenarios of increased bioenergy crop production in an 80-km radius agricultural landscape centered on a potential biomass-processing energy facility and evaluated the consequences of each scenario for bird communities. Our scenarios included conversion of existing annual row crops to perennial bioenergy grasslands and conversion of existing grasslands to annual bioenergy row crops. The scenarios explored combinations of four biomass crop types (three potential grassland crops along a gradient of plant diversity and one annual row crop [corn]), three land conversion percentages to bioenergy crops (10%, 20%, or 30% of row crops or grasslands), and three spatial configurations of biomass crop fields (random, clustered near similar field types, or centered on the processing plant), yielding 36 scenarios. For each scenario, we predicted the impact on four bird community metrics: species richness, total bird density, species of greatest conservation need (SGCN) density, and SGCN hotspots (SGCN birds/ha ≥ 2). Bird community metrics consistently increased with conversion of row crops to bioenergy grasslands and consistently decreased with conversion of grasslands to bioenergy row crops. Spatial arrangement of bioenergy fields had strong effects on the bird community and in some cases was more influential than the amount converted to bioenergy crops. Clustering grasslands had a stronger positive influence on the bird community than locating grasslands near the central plant or at random. Expansion of bioenergy grasslands onto marginal agricultural lands will

  3. The NOAA Near Real-time OMI-SO2 Cloud Visualization and Product Distribution System

    NASA Astrophysics Data System (ADS)

    Vicente, G.; Serafino, G.; Krueger, A.; Carn, S.; Yang, K.; Krotkov, N.; Guffanti, M.; Levelt, P.

    2007-12-01

    The Ozone Monitoring Instrument (OMI) on the NASA EOS/Aura research satellite allows measurement of SO2 concentrations at UV wavelengths with daily global coverage. SO2 is detected from space using its strong absorption band structure in the near UV (300-320 nm) as well as in IR bands near 7.3 and 8.6 mm. Thirty years of UV SO2 measurements with the Total Ozone Mapping Spectrometer (TOMS) and OMI sensors have shown that the highest concentrations of SO2 occur in volcanic clouds produced by explosive magmatic eruptions, which also emit ash. However, icing of ash particles in water-rich eruption clouds, and/or suppression of the IR split- window signal by ambient water vapor or cloud opacity can inhibit direct detection of ash from space. Large SO2 concentrations are therefore a reliable indicator of the presence of airborne volcanic ash. UV SO2 measurements are very robust and are insensitive to the factors that confound IR data. SO2 and ash can be detected in a very fresh eruption cloud due to sunlight backscattering and ash presence can be confirmed by UV derived aerosol index measurements. The lack of other large point sources of SO2 facilitates development and implementation of automated searches for volcanic clouds with a very low false alarm rate. The NASA Earth Sciences Applications Office has funded a cooperative agreement between UMBC, NOAA, GSFC, and USGS to infuse research satellite SO2 data products into volcanic hazard Decision Support Systems (DSSs) operated by the National Oceanic and Atmospheric Administration (NOAA) and the US Geological Survey (USGS). This will provide aviation alerts to the Federal Aviation Administration (FAA), that will reduce false alarms and permit more robust detection and tracking of volcanic clouds, and includes the development of an eruption alarm system, and potential recognition of pre-eruptive volcanic degassing. Near real-time (NRT) observations of SO2 and volcanic ash can therefore be incorporated into data products

  4. Real-time measurements of endogenous CO production from vascular cells using an ultrasensitive laser sensor

    NASA Technical Reports Server (NTRS)

    Morimoto, Y.; Durante, W.; Lancaster, D. G.; Klattenhoff, J.; Tittel, F. K.

    2001-01-01

    Carbon monoxide (CO) has been implicated as a biological messenger molecule analogous to nitric oxide. A compact gas sensor based on a midinfrared laser absorption spectroscopy was developed for direct and real-time measurement of trace levels (in approximate pmol) of CO release by vascular cells. The midinfrared light is generated by difference frequency mixing of two nearinfrared lasers in a nonlinear optical crystal. A strong infrared absorption line of CO (4.61 microm) is chosen for convenient CO detection without interference from other gas species. The generation of CO from cultured vascular smooth muscle cells was detected every 20 s without any chemical modification to the CO. The sensitivity of the sensor reached 6.9 pmol CO. CO synthesis was measured from untreated control cells (0.25 nmol per 10(7) cells/h), sodium nitroprusside-treated cells (0.29 nmol per 10(7) cells/h), and hemin-treated cells (0.49 nmol per 10(7) cells/h). The sensor also detected decreases in CO production after the addition of the heme oxygenase (HO) inhibitor tin protoporphyrin-IX (from 0.49 to 0.02 nmol per 10(7) cells/h) and increases after the administration of the HO substrate hemin (from 0.27 to 0.64 nmol per 10(7) cells/h). These results demonstrate that midinfrared laser absorption spectroscopy is a useful technique for the noninvasive and real-time detection of trace levels of CO from biological tissues.

  5. Real-time measurements of endogenous CO production from vascular cells using an ultrasensitive laser sensor.

    PubMed

    Morimoto, Y; Durante, W; Lancaster, D G; Klattenhoff, J; Tittel, F K

    2001-01-01

    Carbon monoxide (CO) has been implicated as a biological messenger molecule analogous to nitric oxide. A compact gas sensor based on a midinfrared laser absorption spectroscopy was developed for direct and real-time measurement of trace levels (in approximate pmol) of CO release by vascular cells. The midinfrared light is generated by difference frequency mixing of two nearinfrared lasers in a nonlinear optical crystal. A strong infrared absorption line of CO (4.61 microm) is chosen for convenient CO detection without interference from other gas species. The generation of CO from cultured vascular smooth muscle cells was detected every 20 s without any chemical modification to the CO. The sensitivity of the sensor reached 6.9 pmol CO. CO synthesis was measured from untreated control cells (0.25 nmol per 10(7) cells/h), sodium nitroprusside-treated cells (0.29 nmol per 10(7) cells/h), and hemin-treated cells (0.49 nmol per 10(7) cells/h). The sensor also detected decreases in CO production after the addition of the heme oxygenase (HO) inhibitor tin protoporphyrin-IX (from 0.49 to 0.02 nmol per 10(7) cells/h) and increases after the administration of the HO substrate hemin (from 0.27 to 0.64 nmol per 10(7) cells/h). These results demonstrate that midinfrared laser absorption spectroscopy is a useful technique for the noninvasive and real-time detection of trace levels of CO from biological tissues. PMID:11123266

  6. Intensity Maps Production Using Real-Time Joint Streaming Data Processing From Social and Physical Sensors

    NASA Astrophysics Data System (ADS)

    Kropivnitskaya, Y. Y.; Tiampo, K. F.; Qin, J.; Bauer, M.

    2015-12-01

    Intensity is one of the most useful measures of earthquake hazard, as it quantifies the strength of shaking produced at a given distance from the epicenter. Today, there are several data sources that could be used to determine intensity level which can be divided into two main categories. The first category is represented by social data sources, in which the intensity values are collected by interviewing people who experienced the earthquake-induced shaking. In this case, specially developed questionnaires can be used in addition to personal observations published on social networks such as Twitter. These observations are assigned to the appropriate intensity level by correlating specific details and descriptions to the Modified Mercalli Scale. The second category of data sources is represented by observations from different physical sensors installed with the specific purpose of obtaining an instrumentally-derived intensity level. These are usually based on a regression of recorded peak acceleration and/or velocity amplitudes. This approach relates the recorded ground motions to the expected felt and damage distribution through empirical relationships. The goal of this work is to implement and evaluate streaming data processing separately and jointly from both social and physical sensors in order to produce near real-time intensity maps and compare and analyze their quality and evolution through 10-minute time intervals immediately following an earthquake. Results are shown for the case study of the M6.0 2014 South Napa, CA earthquake that occurred on August 24, 2014. The using of innovative streaming and pipelining computing paradigms through IBM InfoSphere Streams platform made it possible to read input data in real-time for low-latency computing of combined intensity level and production of combined intensity maps in near-real time. The results compare three types of intensity maps created based on physical, social and combined data sources. Here we correlate

  7. Global Near Real-Time MODIS and Landsat Flood Mapping and Product Delivery

    NASA Astrophysics Data System (ADS)

    Policelli, F. S.; Slayback, D. A.; Tokay, M. M.; Brakenridge, G. R.

    2014-12-01

    Flooding is the most destructive, frequent, and costly natural disaster faced by modern society, and is increasing in frequency and damage (deaths, displacements, and financial costs) as populations increase and climate change generates more extreme weather events. When major flooding events occur, the disaster management community needs frequently updated and easily accessible information to better understand the extent of flooding and coordinate response efforts. With funding from NASA's Applied Sciences program, we developed and are now operating a near real-time global flood mapping system to help provide flood extent information within 24-48 hours of events. The principal element of the system applies a water detection algorithm to MODIS imagery, which is processed by the LANCE (Land Atmosphere Near real-time Capability for EOS) system at NASA Goddard within a few hours of satellite overpass. Using imagery from both the Terra (10:30 AM local time overpass) and Aqua (1:30 PM) platforms allows the system to deliver an initial daily assessment of flood extent by late afternoon, and more robust assessments after accumulating cloud-free imagery over several days. Cloud cover is the primary limitation in detecting surface water from MODIS imagery. Other issues include the relatively coarse scale of the MODIS imagery (250 meters) for some events, the difficulty of detecting flood waters in areas with continuous canopy cover, confusion of shadow (cloud or terrain) with water, and accurately identifying detected water as flood as opposed to normal water extent. We are working on improvements to address these limitations. We have also begun delivery of near real time water maps at 30 m resolution from Landsat imagery. Although Landsat is not available daily globally, but only every 8 days if imagery from both operating platforms (Landsat 7 and 8) is accessed, it can provide useful higher resolution data on water extent when a clear acquisition coincides with an active

  8. MODIS NDVI Change Detection Techniques and Products Used in the Near Real Time ForWarn System for Detecting, Monitoring, and Analyzing Regional Forest Disturbances

    NASA Technical Reports Server (NTRS)

    Spruce, Joseph P.; Hargrove, William; Gasser, Jerry; Smoot, James; Kuper, Philip D.

    2014-01-01

    This presentation discusses MODIS NDVI change detection methods and products used in the ForWarn Early Warning System (EWS) for near real time (NRT) recognition and tracking of regionally evident forest disturbances throughout the conterminous US (CONUS). The latter has provided NRT forest change products to the forest health protection community since 2010, using temporally processed MODIS Aqua and Terra NDVI time series data to currently compute and post 6 different forest change products for CONUS every 8 days. Multiple change products are required to improve detectability and to more fully assess the nature of apparent disturbances. Each type of forest change product reports per pixel percent change in NDVI for a given 24 day interval, comparing current versus a given historical baseline NDVI. EMODIS 7 day expedited MODIS MOD13 data are used to obtain current and historical NDVIs, respectively. Historical NDVI data is processed with Time Series Product Tool (TSPT); and 2) the Phenological Parameters Estimation Tool (PPET) software. While each change products employ maximum value compositing (MVC) of NDVI, the design of specific products primarily differs in terms of the historical baseline. The three main change products use either 1, 3, or all previous years of MVC NDVI as a baseline. Another product uses an Adaptive Length Compositing (ALC) version of MVC to derive an alternative current NDVI that is the freshest quality NDVI as opposed to merely the MVC NDVI across a 24 day time frame. The ALC approach can improve detection speed by 8 to 16 days. ForWarn also includes 2 change products that improve detectability of forest disturbances in lieu of climatic fluctuations, especially in the spring and fall. One compares current MVC NDVI to the zonal maximum under the curve NDVI per pheno-region cluster class, considering all previous years in the MODIS record. The other compares current maximum NDVI to the mean of maximum NDVI for all previous MODIS years.

  9. Latex Allergies: A Review of Recognition, Evaluation, Management, Prevention, Education, and Alternative Product Use

    PubMed Central

    Schroyer, Traci; Catalfano, Jennifer

    2003-01-01

    Objectives: To provide information about latex allergies; to determine how to recognize, evaluate, and manage emergencies related to latex allergies; and how to identify those at risk for latex allergies. Additionally, ways to prevent latex exposure, to educate health care workers and athletes about latex allergy, and to provide safe alternatives to latex are investigated. Data Sources: We searched MEDLINE and SPORT Discus for the years 1998–2002 using the key words latex, allergies, rubber, anaphylaxis, gloves, cross-reaction, IgE (immunoglobulin G) proteins, and radioallergosorbent test (RAST). Background: Latex, a sap from the rubber tree, is found in many products used in everyday life. Latex is composed of compounds that may cause an allergic reaction, whose severity can range from irritant dermatitis to type IV dermatitis to type I systemic reaction. Recognition of the signs and symptoms associated with these reactions by the health care professional may help to prevent a more severe reaction from occurring. Reactions can be complicated by contact with other substances, thus causing a cross-reaction. Some individuals are more at risk of latex allergies due to repetitive exposure to latex through their career paths, multiple surgeries, other allergies, or respiratory conditions. Management of an acute reaction involves removal of the irritant, cleansing of the affected area, monitoring vital signs for changes, and seeking additional medical assistance as warranted. Recommendations: Those at risk may be identified through a thorough medical history and allergy testing. Prevention techniques and guidelines are examined, with an emphasis on education at multiple levels. Product information for nonlatex equipment and supplies for the athletic training room is offered, with additional resource information provided. PMID:16558678

  10. Berry Leaves: An Alternative Source of Bioactive Natural Products of Nutritional and Medicinal Value†

    PubMed Central

    Ferlemi, Anastasia-Varvara; Lamari, Fotini N.

    2016-01-01

    Berry fruits are recognized, worldwide, as “superfoods” due to the high content of bioactive natural products and the health benefits deriving from their consumption. Berry leaves are byproducts of berry cultivation; their traditional therapeutic use against several diseases, such as the common cold, inflammation, diabetes, and ocular dysfunction, has been almost forgotten nowadays. Nevertheless, the scientific interest regarding the leaf composition and beneficial properties grows, documenting that berry leaves may be considered an alternative source of bioactives. The main bioactive compounds in berry leaves are similar as in berry fruits, i.e., phenolic acids and esters, flavonols, anthocyanins, and procyanidins. The leaves are one of the richest sources of chlorogenic acid. In various studies, these secondary metabolites have demonstrated antioxidant, anti-inflammatory, cardioprotective, and neuroprotective properties. This review focuses on the phytochemical composition of the leaves of the commonest berry species, i.e., blackcurrant, blackberry, raspberry, bilberry, blueberry, cranberry, and lingonberry leaves, and presents their traditional medicinal uses and their biological activities in vitro and in vivo. PMID:27258314

  11. Examination of an alternative method for the pyrometallurgical production of copper-chromium alloys

    NASA Astrophysics Data System (ADS)

    Brenk, J.; Hassan-Pour, S.; Spiess, P.; Friedrich, B.

    2016-07-01

    In this paper an alternative to the usual route for the production of copper-chromium alloys by Hot Isostatic Pressing (HIP) followed by Vacuum Arc Remelting (VAR) is investigated. Therefore the HIP is replaced by an aluminothermic reduction. As oxidizing agents for this aluminothermic reduction (ATR) chromium oxide (Cr2O3) and copper oxide (CuO) are used. These oxidants are mixed in a stoichiometric relation with aluminium (Al) to get the following aluminothermic reactions going: As ATRs always are exothermic reductions, it is important to control the heat output of the reaction. A common simplification for the heat calculation in the field of ATR is the “Shemshushny Factor” (Sh-Factor). This factor determines the rate of energy input per reactant mass: To avoid a secondary melt phase after ATR, inline casting is used to directly obtain vacuum arc remeltable electrodes out of the liquid melt of the ATR. The second part of this work deals with the remelting of these electrodes via VAR. The first VAR trials aim at finding process parameters for remelting a copper-chromium ingot. As demixing is to avoid, it is necessary to control process parameters within a small range to obtain a shallow melt pool.

  12. Selection and properties of alternative forming fluids for TRISO fuel kernel production

    SciTech Connect

    Baker, M. P.; King, J. C.; Gorman, B. P.; Marshall, Doug W.

    2013-01-01

    Current Very High Temperature Reactor (VHTR) designs incorporate TRi-structural ISOtropic (TRISO) fuel, which consists of a spherical fissile fuel kernel surrounded by layers of pyrolytic carbon and silicon carbide. An internal sol-gel process forms the fuel kernel using wet chemistry to produce uranium oxyhydroxide gel spheres by dropping a cold precursor solution into a hot column of trichloroethylene (TCE). Over time, gelation byproducts inhibit complete gelation, and the TCE must be purified or discarded. The resulting TCE waste stream contains both radioactive and hazardous materials and is thus considered a mixed hazardous waste. Changing the forming fluid to a non-hazardous alternative could greatly improve the economics of TRISO fuel kernel production. Selection criteria for a replacement forming fluid narrowed a list of ~10,800 chemicals to yield ten potential replacement forming fluids: 1-bromododecane, 1- bromotetradecane, 1-bromoundecane, 1-chlorooctadecane, 1-chlorotetradecane, 1-iododecane, 1-iodododecane, 1-iodohexadecane, 1-iodooctadecane, and squalane. The density, viscosity, and surface tension for each potential replacement forming fluid were measured as a function of temperature between 25 °C and 80 °C. Calculated settling velocities and heat transfer rates give an overall column height approximation. 1-bromotetradecane, 1-chlorooctadecane, and 1-iodododecane show the greatest promise as replacements, and future tests will verify their ability to form satisfactory fuel kernels.

  13. Alternate routes for the production of fuels from coal and natural gas

    SciTech Connect

    Gray, D.; Tomlinson, G.; ElSawy, A.

    1994-06-01

    Almost all transportation worldwide is powered by high energy-density liquid hydrocarbon fuels produced from crude oil. Transportation fuels currently use over 50 percent of total world petroleum demand of 66 million barrels per day. Prior MITRE studies indicate that crude oil supply will become severely limited after the year 2030 as increasing world energy demand, driven by population growth and economic development, depletes oil resources. If conventional liquid hydrocarbon fuels that can use existing production and distribution infrastructures are still needed for transportation in the future, then alternate sources of these fuels will have to be utilized. Two such sources are natural gas and coal. Natural gas reserves worldwide are expected to last well into the 21st century, and coal resources are enormous. This paper examines the technologies for producing environmentally superior liquid transportation fuels from coal and natural gas using modern conversion technologies. Estimates of the costs of fuels from these sources are given, and the potential environmental impacts of these fuels are examined.

  14. Lysozyme as an alternative to growth promoting antibiotics in swine production.

    PubMed

    Oliver, W T; Wells, J E

    2015-01-01

    Lysozyme is a naturally occurring enzyme found in bodily secretions such as tears, saliva, and milk. It functions as an antimicrobial agent by cleaving the peptidoglycan component of bacterial cell walls, which leads to cell death. Antibiotics are also antimicrobials and have been fed at subtherapeutic levels to swine as growth promoters. These compounds benefit swine producers by minimizing production losses by increasing feed efficiency and decreasing susceptibility to bacterial infection and disease. This manuscript reviews the knowledge of the effects of lysozyme, as compared to traditional subtherapeutic antibiotics in swine feed, on pig performance and health. It is clear from decades of studies that antibiotic use in feeds increases pig performance, particularly in the nursery. Similarly, lysozyme, as a feed additive, increases growth and feed efficiency. While the mechanism by which antibiotics and lysozyme improve performance is not clearly understood, both of these feed additives improve gastrointestinal health, improve the metabolic profile, and alter the gastrointestinal bacteria ecology of swine. Therefore, lysozyme is a suitable alternative to growth-promoting subtherapeutic antibiotic use in swine feed. PMID:26273432

  15. Review on emu products for use as complementary and alternative medicine.

    PubMed

    Jeengar, Manish Kumar; Kumar, P Sravan; Thummuri, Dinesh; Shrivastava, Shweta; Guntuku, Lalita; Sistla, Ramakrishna; Naidu, V G M

    2015-01-01

    Emu (Dromaius novaehallandiae), the flightless bird native to Australia and found in many countries, is receiving much attention for its nutritional benefits as well as its medicinal value. Emu oil contains high amounts of polyunsaturated fatty acids and antioxidants. It has potent anti-inflammatory actions and thus can be used topically and orally to treat conditions such as mucositis, inflammatory bowel syndrome, and auricular inflammation, and to prevent chemotherapy-induced bone loss. Emu oil also has a hypocholesterolemic effect, transdermal penetration-enhancing activity, cosmetic and insect repellent activity, and so on. However, its mechanism(s) of actions are unclear and have not, to our knowledge, been studied to date. Previous studies suggest that the fatty acids of the ω-9, ω-6, and ω-3 series, which are present in emu oil, may act on cyclooxygenase, lipoxygenase, and lipoxin pathways to bring about its anti-inflammatory and other beneficial actions. The aim of this review was to provide a brief summary of the current knowledge of research on emu products, mainly emu oil, for the possible use as a complementary and alternative natural medicine for various chronic diseases. In this review we also highlighted the future research scope of emu oil for its possible antidiabetic activity. Thus, emu oil is an attractive pharmacologic agent to further explore for its therapeutic activity to treat various ailments. PMID:25441585

  16. Selection and properties of alternative forming fluids for TRISO fuel kernel production

    NASA Astrophysics Data System (ADS)

    Baker, M. P.; King, J. C.; Gorman, B. P.; Marshall, D. W.

    2013-01-01

    Current Very High Temperature Reactor (VHTR) designs incorporate TRi-structural ISOtropic (TRISO) fuel, which consists of a spherical fissile fuel kernel surrounded by layers of pyrolytic carbon and silicon carbide. An internal sol-gel process forms the fuel kernel using wet chemistry to produce uranium oxyhydroxide gel spheres by dropping a cold precursor solution into a hot column of trichloroethylene (TCE). Over time, gelation byproducts inhibit complete gelation, and the TCE must be purified or discarded. The resulting TCE waste stream contains both radioactive and hazardous materials and is thus considered a mixed hazardous waste. Changing the forming fluid to a non-hazardous alternative could greatly improve the economics of TRISO fuel kernel production. Selection criteria for a replacement forming fluid narrowed a list of ˜10,800 chemicals to yield ten potential replacement forming fluids: 1-bromododecane, 1-bromotetradecane, 1-bromoundecane, 1-chlorooctadecane, 1-chlorotetradecane, 1-iododecane, 1-iodododecane, 1-iodohexadecane, 1-iodooctadecane, and squalane. The density, viscosity, and surface tension for each potential replacement forming fluid were measured as a function of temperature between 25 °C and 80 °C. Calculated settling velocities and heat transfer rates give an overall column height approximation. 1-bromotetradecane, 1-chlorooctadecane, and 1-iodododecane show the greatest promise as replacements, and future tests will verify their ability to form satisfactory fuel kernels.

  17. Berry Leaves: An Alternative Source of Bioactive Natural Products of Nutritional and Medicinal Value.

    PubMed

    Ferlemi, Anastasia-Varvara; Lamari, Fotini N

    2016-01-01

    Berry fruits are recognized, worldwide, as "superfoods" due to the high content of bioactive natural products and the health benefits deriving from their consumption. Berry leaves are byproducts of berry cultivation; their traditional therapeutic use against several diseases, such as the common cold, inflammation, diabetes, and ocular dysfunction, has been almost forgotten nowadays. Nevertheless, the scientific interest regarding the leaf composition and beneficial properties grows, documenting that berry leaves may be considered an alternative source of bioactives. The main bioactive compounds in berry leaves are similar as in berry fruits, i.e., phenolic acids and esters, flavonols, anthocyanins, and procyanidins. The leaves are one of the richest sources of chlorogenic acid. In various studies, these secondary metabolites have demonstrated antioxidant, anti-inflammatory, cardioprotective, and neuroprotective properties. This review focuses on the phytochemical composition of the leaves of the commonest berry species, i.e., blackcurrant, blackberry, raspberry, bilberry, blueberry, cranberry, and lingonberry leaves, and presents their traditional medicinal uses and their biological activities in vitro and in vivo. PMID:27258314

  18. Do placebo based validation standards mimic real batch products behaviour? Case studies.

    PubMed

    Bouabidi, A; Talbi, M; Bouklouze, A; El Karbane, M; Bourichi, H; El Guezzar, M; Ziemons, E; Hubert, Ph; Rozet, E

    2011-06-01

    Analytical methods validation is a mandatory step to evaluate the ability of developed methods to provide accurate results for their routine application. Validation usually involves validation standards or quality control samples that are prepared in placebo or reconstituted matrix made of a mixture of all the ingredients composing the drug product except the active substance or the analyte under investigation. However, one of the main concerns that can be made with this approach is that it may lack an important source of variability that come from the manufacturing process. The question that remains at the end of the validation step is about the transferability of the quantitative performance from validation standards to real authentic drug product samples. In this work, this topic is investigated through three case studies. Three analytical methods were validated using the commonly spiked placebo validation standards at several concentration levels as well as using samples coming from authentic batch samples (tablets and syrups). The results showed that, depending on the type of response function used as calibration curve, there were various degrees of differences in the results accuracy obtained with the two types of samples. Nonetheless the use of spiked placebo validation standards was showed to mimic relatively well the quantitative behaviour of the analytical methods with authentic batch samples. Adding these authentic batch samples into the validation design may help the analyst to select and confirm the most fit for purpose calibration curve and thus increase the accuracy and reliability of the results generated by the method in routine application. PMID:21377822

  19. Efficient DMFT impurity solver using real-time dynamics with matrix product states

    NASA Astrophysics Data System (ADS)

    Ganahl, Martin; Aichhorn, Markus; Evertz, Hans Gerd; Thunström, Patrik; Held, Karsten; Verstraete, Frank

    2015-10-01

    We propose to calculate spectral functions of quantum impurity models using the time evolving block decimation (TEBD) for matrix product states. The resolution of the spectral function is improved by a so-called linear prediction approach. We apply the method as an impurity solver within the dynamical mean-field theory (DMFT) for the single- and two-band Hubbard model on the Bethe lattice. For the single-band model, we observe sharp features at the inner edges of the Hubbard bands. A finite-size scaling shows that they remain present in the thermodynamic limit. We analyze the real time-dependence of the double occupation after adding a single electron and observe oscillations at the same energy as the sharp feature in the Hubbard band, indicating a long-lived coherent superposition of states that correspond to the Kondo peak and the side peaks. For a two-band Hubbard model, we observe an even richer structure in the Hubbard bands, which cannot be related to a multiplet structure of the impurity, in addition to sharp excitations at the band edges of a type similar to the single-band case.

  20. Part 4 of a 4-part series Miscellaneous Products: Trends and Alternatives in Deodorants, Antiperspirants, Sunblocks, Shaving Products, Powders, and Wipes

    PubMed Central

    Jacob, Sharon; Katta, Rajani; Nedorost, Susan; Warshaw, Erin; Zirwas, Matt; Selbo, Nicole

    2011-01-01

    Objective: To provide updated data on the usage of ingredients that are common potential contact allergens in several categories of topical products. To identify useful alternative products with few or no common contact allergens. Design: In November 2009, the full ingredient lists of 5,416 skin, hair, and cosmetic products marketed by the CVS pharmacy chain were copied from CVS.com into Microsoft Word format for analysis. Computer searches were made in Microsoft Word using search/replace and sorting functions to accurately identify the presence of specific allergens in each website product. Measurements: Percentages of American Contact Dermatitis Society core series allergens (and other common preservatives and sunblocks) were calculated. Results: The usage of American Contact Dermatitis Society core series allergens (and other preservatives and sunblocks) in various miscellaneous categories of topical products is reported. Conclusion: Data on allergens and alternatives for ancillary skin care products are not widely published. This article reviews some of the common potential allergens in antiperspirants, deodorants, shaving products, sunblocks, powders, and wipes. Suitable available alternative products for patients with contact allergy are listed. PMID:22010054

  1. Real-Time PCR Methods for Detection of Foodborne Bacterial Pathogens in Meat and Meat Products

    NASA Astrophysics Data System (ADS)

    Hernández, Marta; Hansen, Flemming; Cook, Nigel; Rodríguez-Lázaro, David

    As a consequence of the potential hazards posed by the presence of microbial pathogens, microbiological quality control programmes are being increasingly applied throughout the meat production chain in order to minimize the risk of infection for the consumer. Classical microbiological methods to detect the presence of microorganisms, involving enrichment and isolation of presumptive colonies of bacteria on solid media, and final confirmation by biochemical and/or serological identification, although remaining the approach of choice in routine analytical laboratories, can be laborious and time consuming. The adoption of molecular techniques in microbial diagnostics has become a promising alternative approach, as they possess inherent advantages such as shorter time to results, excellent detection limits, specificity and potential for automation. Several molecular detection techniques have been devised in the last two decades, such as nucleic acid sequence-based amplification (NASBA) (Cook, 2003; Rodriguez-Lazaro, Hernandez, D’Agostino, & Cook, 2006) and loop-mediated isothermal amplification (Notomi et al., 2000), but the one which has undergone the most extensive development as a practical food analytical tool is the polymerase chain reaction (PCR) (Hoorfar & Cook, 2003; Malorny, Tassios, et al., 2003).

  2. 21 CFR 201.26 - Exceptions or alternatives to labeling requirements for human drug products held by the Strategic...

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... 21 Food and Drugs 4 2014-04-01 2014-04-01 false Exceptions or alternatives to labeling requirements for human drug products held by the Strategic National Stockpile. 201.26 Section 201.26 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL LABELING General Labeling Provisions §...

  3. Personal computer guide for selecting alternative coatings for metal parts and products painting. Report for July 1995--January 1996

    SciTech Connect

    Cornstubble, D.R.; Baskir, K.N.; Kosusko, M.

    1996-01-01

    The paper describes the principles of the Coating Alternatives Guide (CAGE) program and the development of its Beta version for metal parts and products painting. The paper discusses the background of CAGE, the content and function of the CAGE program, the current needs of the program, and the long term goals of CAGE development.

  4. Grammatical Planning Units during Real-Time Sentence Production in Speakers with Agrammatic Aphasia and Healthy Speakers

    ERIC Educational Resources Information Center

    Lee, Jiyeon; Yoshida, Masaya; Thompson, Cynthia K.

    2015-01-01

    Purpose: Grammatical encoding (GE) is impaired in agrammatic aphasia; however, the nature of such deficits remains unclear. We examined grammatical planning units during real-time sentence production in speakers with agrammatic aphasia and control speakers, testing two competing models of GE. We queried whether speakers with agrammatic aphasia…

  5. MAp19, the alternative splice product of the MASP2 gene.

    PubMed

    Degn, Søren E; Thiel, Steffen; Nielsen, Ole; Hansen, Annette G; Steffensen, Rudi; Jensenius, Jens C

    2011-10-28

    The lectin pathway of complement is a central part of innate immunity, but as a powerful inducer of inflammation it needs to be tightly controlled. The MASP2 gene encodes two proteins, MASP-2 and MAp19. MASP-2 is the serine protease responsible for lectin pathway activation. The smaller alternative splice product, MAp19, lacks a catalytic domain but retains two of three domains involved in association with the pattern-recognition molecules (PRMs): mannan-binding lectin (MBL), H-ficolin, L-ficolin and M-ficolin. MAp19 reportedly acts as a competitive inhibitor of MASP-2-mediated complement activation. In light of a ten times lower affinity of MAp19, versus MASP-2, for association with the PRMs, much higher serum concentrations of MAp19 than MASP-2 would be required for MAp19 to exert such an inhibitory activity. Just four amino acid residues distinguish MAp19 from MASP-2, and these are conserved between man, mouse and rat. Nonetheless we generated monoclonal rat anti-MAp19 antibodies and established a quantitative assay. We found the concentration of MAp19 in serum to be 217 ng/ml, i.e., 11nM, comparable to the 7 nM of MASP-2. In serum all MASP-2, but only a minor fraction of MAp19, was associated with PRMs. In contrast to previous reports we found that MAp19 could not compete with MASP-2 for binding to MBL, nor could it inhibit MASP-2-mediated complement activation. Immunohistochemical analyses combined with qRT-PCR revealed that both MAp19 and MASP-2 were mainly expressed in hepatocytes. High levels of MAp19 were found in urine, where MASP-2 was absent. PMID:21871896

  6. Alternate Wetting and Drying as an Effective Management Practice to Reduce Methane in Arkansas Rice Production

    NASA Astrophysics Data System (ADS)

    Runkle, B.; Smith, S. F.; Suvocarev, K.; Reba, M. L.

    2015-12-01

    Approximately 15% of the global 308 Tg CH4 emitted by anthropogenic sources is currently attributed to rice cultivation. Arkansas, the leading state in rice cultivation, produces over 42% of the total rice and represents over 43% of total land planted to rice in the US. Although rice production is generally water-intensive, some rice producers have adopted a conservation practice, 'Alternate Wetting and Drying' (AWD), in which the flood is released periodically during the growing season. In addition, implementing AWD can reduce CH4 emissions though the introduction of aerobic conditions. To assess the magnitude of this reduction, conventionally flooded (CONV) and AWD fields were identically instrumented for the 2015 season and fluxes of CH4 were measured with an open path IRGA. Other biophysical variables were monitored to determine the relative dominance of potential drivers. Half-hourly CH4 fluxes from the AWD and CONV fields during their similar initial flood (DOY 138-161) were well correlated (R2 = 0.762), indicating similar mechanisms controlling CH4 emissions in both fields. After the initial drydown event in the AWD field (162 DOY), daily median CH4 fluxes continued to rise to 7.80 mg CH4 m-2 h-1 on 163 DOY before subsiding to a local minimum of 0.162 mg CH4 m-2 h-1 on 171 DOY. Daily median CH4 fluxes between 9.24 and 16.0 mg CH4 m-2 h-1 were observed in the CONV field during this same period. Cumulative emissions from both fields following the drydown event and prior to rewetting demonstrated a reduction in CH4 emissions by the AWD treatment by 82%. The substantial decrease in CH4 emissions by AWD in the early growing season supports and expands upon previous chamber-based research and offers strong evidence for the efficacy of AWD in reducing CH4 emissions in AR rice production. The presentation will also assess the latter portion of the growing season, currently underway, and will provide process-based relationships between biophysical parameters and CH

  7. Primary healthcare provider knowledge, beliefs and clinic-based practices regarding alternative tobacco products and marijuana: a qualitative study.

    PubMed

    Bascombe, Ta Misha S; Scott, Kimberly N; Ballard, Denise; Smith, Samantha A; Thompson, Winifred; Berg, Carla J

    2016-06-01

    Use prevalence of alternative tobacco products and marijuana has increased dramatically. Unfortunately, clinical guidelines have focused on traditional cigarettes with limited attention regarding these emerging public health issues. Thus, it is critical to understand how healthcare professionals view this issue and are responding to it. This qualitative study explored knowledge, beliefs and clinic-based practices regarding traditional and alternative tobacco products (cigar-like products, smokeless tobacco, hookah, e-cigarettes) and marijuana among rural and urban Georgia primary healthcare providers. The sample comprised 20 healthcare providers in primary care settings located in the Atlanta Metropolitan area and rural southern Georgia who participated in semi-structured interviews. Results indicated a lack of knowledge about these products, with some believing that some products were less harmful than traditional cigarettes or that they may be effective in promoting cessation or harm reduction. Few reported explicitly assessing use of these various products in clinic. In addition, healthcare providers reported a need for empirical evidence to inform their clinical practice. Healthcare providers must systematically assess use of the range of tobacco products and marijuana. Evidence-based recommendations or information sources are needed to inform clinical practice and help providers navigate conversations with patients using or inquiring about these products. PMID:26802106

  8. Rapid detection of Listeria monocytogenes by real-time PCR in processed meat and dairy products.

    PubMed

    Heo, Eun Jeong; Song, Bo Ra; Park, Hyun Jung; Kim, Young Jo; Moon, Jin San; Wee, Sung Hwan; Kim, Jin-Seok; Yoon, Yohan

    2014-03-01

    The objectives of this study were to evaluate the detection of Listeria monocytogenes in different ready-to-eat foods using real-time PCR (RT-PCR). Various concentrations (10(0) to 10(5) CFU/ml) of L. monocytogenes ATCC 19115 were inoculated into ham, sausage, ground meat, processed milk, cheese, and infant formula. L. monocytogenes ATCC 19115 in the samples was then enumerated on Oxford agar, and DNA was extracted from the samples before and after incubation at 36°C for 4 h. A set of primers and hybridization probe designed in this study was then used to detect the pathogen. The standard curve was then prepared by plotting cycle threshold values for each dilution versus L. monocytogenes cell counts (log CFU). The specificity of the set of primers and hybridization probe was appropriate. A 4-h incubation at 36°C before DNA extraction produced optimum standard curves in comparison to the results for a 0-h incubation. Thus, a 4-h incubation at 36°C was applied for monitoring L. monocytogenes in collected food samples. To monitor L. monocytogenes in foods, 533 samples (ham, 129; sausage, 226; ground meat, 72; processed cheese, 54; processed milk, 42; and infant formula, 10) were collected from retail markets and from the step before pasteurization in plants. Of all 533 samples, 4 samples (0.8%) showed positive signals in RT-PCR. Two samples from hams (1.6%) and two samples from sausages (0.9%) were determined to be positive for L. monocytogenes at < 100 CFU/g. The results indicate that the RT-PCR detection method with the set of primers and hybridization probe designed in this study should be useful in monitoring for L. monocytogenes in processed meat and milk products. PMID:24674437

  9. CALIOP near-real-time backscatter products compared to EARLINET data

    NASA Astrophysics Data System (ADS)

    Grigas, T.; Hervo, M.; Gimmestad, G.; Forrister, H.; Schneider, P.; Preißler, J.; Tarrason, L.; O'Dowd, C.

    2015-11-01

    The expedited near-real-time Level 1.5 Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) version 3 products were evaluated against data from the ground-based European Aerosol Research Lidar Network (EARLINET). The statistical framework and results of the three-year evaluation of 48 CALIOP overpasses with ground tracks within a 100 km distance from operating EARLINET stations are presented and include analysis for the following CALIOP classifications of aerosol type: dust, polluted dust, clean marine, clean continental, polluted continental, mixed and/or smoke/biomass burning. For the complete data set comprising both the planetary boundary layer (PBL) and the free troposphere (FT) data, the correlation coefficient (R) was 0.86. When the analysis was conducted separately for the PBL and FT, the correlation coefficients were R = 0.6 and R = 0.85, respectively. From analysis of selected specific cases, it was initially thought that the presence of FT layers, with high attenuated backscatter, led to poor agreement of the PBL backscatter profiles between the CALIOP and EARLINET and prompted a further analysis to filter out such cases; however, removal of these layers did not improve the agreement as R reduced marginally from R = 0.86 to R = 0.84 for the combined PBL and FT analysis, increased marginally from R = 0.6 up to R = 0.65 for the PBL on its own, and decreased marginally from R = 0.85 to R = 0.79 for the FT analysis on its own. This suggests considerable variability, across the data set, in the spatial distribution of the aerosol over spatial scales of 100 km or less around some EARLINET stations rather than influence from elevated FT layers. For specific aerosol types, the correlation coefficient between CALIOP backscatter profiles and the EARLINET data ranged from R = 0.37 for polluted continental aerosol in the PBL to R = 0.57 for dust in the FT.

  10. CALIOP near-real-time backscatter products compared to EARLINET data

    NASA Astrophysics Data System (ADS)

    Grigas, T.; Hervo, M.; Gimmestad, G.; Forrister, H.; Schneider, P.; Preißler, J.; Tarrason, L.; O'Dowd, C.

    2015-03-01

    The expedited near-real-time Level 1.5 Cloud-Aerosol Lidar (Light Detection and Ranging) with Orthogonal Polarization (CALIOP) products were evaluated against data from the ground-based European Aerosol Research Lidar Network (EARLINET). Over a period of three years, lidar data from 48 CALIOP overpasses with ground tracks within a 100 km distance from an operating EARLINET station were deemed suitable for analysis and they included a valid aerosol classification type (e.g. dust, polluted dust, clean marine, clean continental, polluted continental, mixed and/or smoke/biomass burning). For the complete dataset comprising both PBL and FT data, the correlation coefficient was 0.86, and when separated into separate layers, the PBL and FT correlation coefficients were 0.6 and 0.85 respectively. The presence of FT layers with high attenuated backscatter led to poor agreement in PBL backscatter profiles between the CALIOP and EARLINET measurements and prompted a further analysis filtering out such cases. However, the correlation coefficient value for the complete dataset decreased marginally from 0.86 to 0.84 while the PBL coefficient increased from 0.6 up to 0.65 and the FT coefficient also decreased from 0.85 to 0.79. For specific aerosol types, the correlation coefficient between CALIOP backscatter profiles and ground-based lidar data ranged from 0.37 for polluted continental aerosol in the planetary boundary layer (PBL) to 0.57 for dust in the free troposphere (FT). The results suggest different levels of agreement based on the location of the dominant aerosol layer and the aerosol type.

  11. Improving food and agriculture productivity and the environment: Canadian initiatives in methyl bromide alternatives and emission control technologies. Revised edition

    SciTech Connect

    Marcotte, M.; Tibelius, C.

    1998-12-31

    Methyl bromide, a fumigant used in the agricultural sector, was listed as an ozone-depleting substance under the Montreal Protocol and is scheduled for phasing out in Canada. This report begins with a review of the joint industry/government approach being taken to plan and manage this phase-out. It then reviews alternative solutions that have been formulated and tested as replacements for the use of methyl bromide in greenhouse cultivation, soil fumigation, strawberry transplant production, tobacco production, grain production, and food processing facilities. Contact names and addresses are provided for those seeking further information. The final sections describe activities in methyl bromide recovery and recycling and list industry and government organizations that have expertise in methyl bromide alternatives.

  12. eMODIS Expedited: Overview of a Near Real Time MODIS Production System for Operational Vegetation Monitoring

    NASA Astrophysics Data System (ADS)

    Jenkerson, C.; Meyer, D. J.; Werpy, J.; Evenson, K.; Merritt, M.

    2010-12-01

    The expedited MODIS, or eMODIS production system derives near-real time Normalized Difference Vegetation Index (NDVI) from Moderate Resolution Imaging Spectroradiometer (MODIS) surface reflectance provided by the Land and Atmosphere Near-real time Capability for EOS (LANCE). There are currently three regions covered by this U.S. Geological Survey (USGS) capability, including the continental U.S., Africa, and the Central America/Caribbean regions. Each of the eMODIS production streams is configured to output its data in map projections, compositing intervals, spatial resolutions, and file formats specific to its region and user community. The challenges of processing 1,000-meter, 500-m, and especially 250-m products by midnight on the last day of a product interval have been met with increasingly effective software and system architecture. An anonymous file transfer protocol (FTP) distribution site (ftp://emodisftp.cr.usgs.gov/eMODIS) allows users direct access to eMODIS NDVI products for operational (near-real time) monitoring of vegetation conditions like drought, crop failure, insect infestation, and other threats, thus supporting subsequent early warning of famine and the targeting of vulnerable populations for insecure food situations.

  13. Simple automatic device for real time sampling of gas production by a reactor

    SciTech Connect

    Frattolillo, A.

    2006-06-15

    An innovative automatic device, allowing periodically drawing samples of the gases produced by a generic reactor, is presented. The gases evolving during the reaction are collected in a storage manifold, equipped with a variable volume consisting of a stainless steel bellow, whose expansion or contraction is driven by a linear step motor. A capacitive gauge monitors the pressure inside the storage manifold, while a feedback control loop reacts to any pressure change adjusting the variable volume (by means of the step motor) in such a way to keep the pressure at a desired set point P{sub 0}. As long as the reaction proceeds, the gas production results in a progressive expansion of the variable volume, whose instantaneous value is constantly monitored by means of a slide potentiometer, whose lever is rigidly connected to the bellow's moving extremity. Once the bellow's expansion has reached a predetermined volume increment {delta}V, which means that an amount of gas P{sub 0}{delta}V has been produced and collected in the storage circuit, a quantity P{sub 0}V{sub S}{<=}P{sub 0}{delta}V of gas is released to the analysis system. A set of electropneumatic valves, automatically operated by the control system, allows for gas delivery to the analysis equipment and retrieval of the set point pressure, by compression of the variable volume, with no influence on the reaction. All relevant parameters are monitored and logged on a personal computer. The control and data acquisition software, made out using National Instrument LABVIEW trade mark sign , also provides control of the analysis equipment. The ability of the proposed setup to not affect the ongoing process allows real time monitoring (by drawing samples at regular time intervals during the reaction) of the gas production. Moreover, since the amount of gas P{sub 0}V{sub S} drawn at each sampling is always the same, it is possible to establish at a glance whether or not there are changes in the concentration of any

  14. Quantitative real-time polymerase chain reaction is an alternative method for the detection of HER-2 amplification in formalin-fixed paraffin-embedded breast cancer samples

    PubMed Central

    Pu, Tianjie; Guo, Peng; Qiu, Yan; Chen, Shinan; Yang, Libo; Sun, Linyong; Ye, Feng; Bu, Hong

    2015-01-01

    Fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC) are the most common methods that are used to quantify HER-2 gene and protein levels, respectively, in human breast cancer. However, due to bad sample quality, some samples are unable to be subjected to a FISH assay. We evaluated 71 formalin-fixed paraffin-embedded (FFPE) breast carcinoma specimens by quantitative real-time polymerase chain reaction (qPCR), IHC, and FISH. We also performed qPCR and FISH assays on delayed formalin-fixed (DDF) samples. The qPCR results were in complete concordance with the results of IHC and FISH. In regards to the DDF samples, the HER-2 fluorescent signal seemed decayed compared with that of the DDF samples after 1 h. However, the qPCR method still works well up to 12 hours. Our results indicated that qPCR was obviously superior to FISH in cases that were not fixed in a reasonable amount of time. However, qPCR can be an alternative method by which to perform HER2 amplification assays in breast cancer. PMID:26617766

  15. Alternative Tobacco Products as a Second Front in the War on Tobacco

    PubMed Central

    Amrock, Stephen M.; Weitzman, Michael

    2015-01-01

    JAMA PEDIATRICS Associations Between Initial Water Pipe Tobacco Smoking and Snus Use and Subsequent Cigarette Smoking: Results From a Longitudinal Study of US Adolescents and Young Adults Samir Soneji, PhD; James D. Sargent, MD; Susanne E. Tanski, MD, MPH; Brian A. Primack, MD, PhD IMPORTANCE Many adolescents and young adults use alternative tobacco products, such as water pipes and snus, instead of cigarettes. OBJECTIVE To assess whether prior water pipe tobacco smoking and snus use among never smokers are risk factors for subsequent cigarette smoking. DESIGN, SETTING, AND PARTICIPANTS We conducted a 2-wave national longitudinal study in the United States among 2541 individuals aged 15 to 23 years old. At baseline (October 25, 2010, through June 11, 2011), we ascertained whether respondents had smoked cigarettes, smoked water pipe tobacco, or used snus. At the 2-year follow-up (October 27, 2012, through March 31, 2013), we determined whether baseline non–cigarette smokers had subsequently tried cigarette smoking, were current (past 30 days) cigarette smokers, or were high-intensity cigarette smokers. We fit multivariable logistic regression models among baseline non–cigarette smokers to assess whether baseline water pipe tobacco smoking and baseline snus use were associated with subsequent cigarette smoking initiation and current cigarette smoking, accounting for established sociodemographic and behavioral risk factors. We fit similarly specified multivariable ordinal logistic regression models to assess whether baseline water pipe tobacco smoking and baseline snus use were associated with high-intensity cigarette smoking at follow-up. EXPOSURES Water pipe tobacco smoking and the use of snus at baseline. MAIN OUTCOMES AND MEASURES Among baseline non–cigarette smokers, cigarette smoking initiation, current (past 30 days) cigarette smoking at follow-up, and the intensity of cigarette smoking at follow-up. RESULTS Among 1596 respondents, 1048 had never smoked

  16. The Real-Valued Sparse Direction of Arrival (DOA) Estimation Based on the Khatri-Rao Product.

    PubMed

    Chen, Tao; Wu, Huanxin; Zhao, Zhongkai

    2016-01-01

    There is a problem that complex operation which leads to a heavy calculation burden is required when the direction of arrival (DOA) of a sparse signal is estimated by using the array covariance matrix. The solution of the multiple measurement vectors (MMV) model is difficult. In this paper, a real-valued sparse DOA estimation algorithm based on the Khatri-Rao (KR) product called the L₁-RVSKR is proposed. The proposed algorithm is based on the sparse representation of the array covariance matrix. The array covariance matrix is transformed to a real-valued matrix via a unitary transformation so that a real-valued sparse model is achieved. The real-valued sparse model is vectorized for transforming to a single measurement vector (SMV) model, and a new virtual overcomplete dictionary is constructed according to the KR product's property. Finally, the sparse DOA estimation is solved by utilizing the idea of a sparse representation of array covariance vectors (SRACV). The simulation results demonstrate the superior performance and the low computational complexity of the proposed algorithm. PMID:27187409

  17. 26 CFR 1.1033(g)-1 - Condemnation of real property held for productive use in trade or business or for investment.

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ... productive use in trade or business or for investment. 1.1033(g)-1 Section 1.1033(g)-1 Internal Revenue... Nontaxable Exchanges § 1.1033(g)-1 Condemnation of real property held for productive use in trade or business... advertising displays as real property—(1) In general. Under section 1033(g)(3) of the Code, a taxpayer...

  18. 26 CFR 1.1033(g)-1 - Condemnation of real property held for productive use in trade or business or for investment.

    Code of Federal Regulations, 2014 CFR

    2014-04-01

    ... productive use in trade or business or for investment. 1.1033(g)-1 Section 1.1033(g)-1 Internal Revenue... (CONTINUED) Common Nontaxable Exchanges § 1.1033(g)-1 Condemnation of real property held for productive use... treat outdoor advertising displays as real property—(1) In general. Under section 1033(g)(3) of the...

  19. 26 CFR 1.1033(g)-1 - Condemnation of real property held for productive use in trade or business or for investment.

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... productive use in trade or business or for investment. 1.1033(g)-1 Section 1.1033(g)-1 Internal Revenue... (CONTINUED) Common Nontaxable Exchanges § 1.1033(g)-1 Condemnation of real property held for productive use... treat outdoor advertising displays as real property—(1) In general. Under section 1033(g)(3) of the...

  20. 26 CFR 1.1033(g)-1 - Condemnation of real property held for productive use in trade or business or for investment.

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... productive use in trade or business or for investment. 1.1033(g)-1 Section 1.1033(g)-1 Internal Revenue... (CONTINUED) Common Nontaxable Exchanges § 1.1033(g)-1 Condemnation of real property held for productive use... treat outdoor advertising displays as real property—(1) In general. Under section 1033(g)(3) of the...

  1. 26 CFR 1.1033(g)-1 - Condemnation of real property held for productive use in trade or business or for investment.

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... productive use in trade or business or for investment. 1.1033(g)-1 Section 1.1033(g)-1 Internal Revenue... (CONTINUED) Common Nontaxable Exchanges § 1.1033(g)-1 Condemnation of real property held for productive use... treat outdoor advertising displays as real property—(1) In general. Under section 1033(g)(3) of the...

  2. Ecology of Fungus Gnats (Bradysia spp.) in Greenhouse Production Systems Associated with Disease-Interactions and Alternative Management Strategies.

    PubMed

    Cloyd, Raymond A

    2015-01-01

    Fungus gnats (Bradysia spp.) are major insect pests of greenhouse-grown horticultural crops mainly due to the direct feeding damage caused by the larvae, and the ability of larvae to transmit certain soil-borne plant pathogens. Currently, insecticides and biological control agents are being used successively to deal with fungus gnat populations in greenhouse production systems. However, these strategies may only be effective as long as greenhouse producers also implement alternative management strategies such as cultural, physical, and sanitation. This includes elimination of algae, and plant and growing medium debris; placing physical barriers onto the growing medium surface; and using materials that repel fungus gnat adults. This article describes the disease-interactions associated with fungus gnats and foliar and soil-borne diseases, and the alternative management strategies that should be considered by greenhouse producers in order to alleviate problems with fungus gnats in greenhouse production systems. PMID:26463188

  3. Ecology of Fungus Gnats (Bradysia spp.) in Greenhouse Production Systems Associated with Disease-Interactions and Alternative Management Strategies

    PubMed Central

    Cloyd, Raymond A.

    2015-01-01

    Fungus gnats (Bradysia spp.) are major insect pests of greenhouse-grown horticultural crops mainly due to the direct feeding damage caused by the larvae, and the ability of larvae to transmit certain soil-borne plant pathogens. Currently, insecticides and biological control agents are being used successively to deal with fungus gnat populations in greenhouse production systems. However, these strategies may only be effective as long as greenhouse producers also implement alternative management strategies such as cultural, physical, and sanitation. This includes elimination of algae, and plant and growing medium debris; placing physical barriers onto the growing medium surface; and using materials that repel fungus gnat adults. This article describes the disease-interactions associated with fungus gnats and foliar and soil-borne diseases, and the alternative management strategies that should be considered by greenhouse producers in order to alleviate problems with fungus gnats in greenhouse production systems. PMID:26463188

  4. A Changing Number of Alternative States in the Boreal Biome: Reproducibility Risks of Replacing Remote Sensing Products.

    PubMed

    Xu, Chi; Holmgren, Milena; Van Nes, Egbert H; Hirota, Marina; Chapin, F Stuart; Scheffer, Marten

    2015-01-01

    Publicly available remote sensing products have boosted science in many ways. The openness of these data sources suggests high reproducibility. However, as we show here, results may be specific to versions of the data products that can become unavailable as new versions are posted. We focus on remotely-sensed tree cover. Recent studies have used this public resource to detect multi-modality in tree cover in the tropical and boreal biomes. Such patterns suggest alternative stable states separated by critical tipping points. This has important implications for the potential response of these ecosystems to global climate change. For the boreal region, four distinct ecosystem states (i.e., treeless, sparse and dense woodland, and boreal forest) were previously identified by using the Collection 3 data of MODIS Vegetation Continuous Fields (VCF). Since then, the MODIS VCF product has been updated to Collection 5; and a Landsat VCF product of global tree cover at a fine spatial resolution of 30 meters has been developed. Here we compare these different remote-sensing products of tree cover to show that identification of alternative stable states in the boreal biome partly depends on the data source used. The updated MODIS data and the newer Landsat data consistently demonstrate three distinct modes around similar tree-cover values. Our analysis suggests that the boreal region has three modes: one sparsely vegetated state (treeless), one distinct 'savanna-like' state and one forest state, which could be alternative stable states. Our analysis illustrates that qualitative outcomes of studies may change fundamentally as new versions of remote sensing products are used. Scientific reproducibility thus requires that old versions remain publicly available. PMID:26571014

  5. A Changing Number of Alternative States in the Boreal Biome: Reproducibility Risks of Replacing Remote Sensing Products

    PubMed Central

    Xu, Chi; Holmgren, Milena; Van Nes, Egbert H.; Hirota, Marina; Chapin, F. Stuart; Scheffer, Marten

    2015-01-01

    Publicly available remote sensing products have boosted science in many ways. The openness of these data sources suggests high reproducibility. However, as we show here, results may be specific to versions of the data products that can become unavailable as new versions are posted. We focus on remotely-sensed tree cover. Recent studies have used this public resource to detect multi-modality in tree cover in the tropical and boreal biomes. Such patterns suggest alternative stable states separated by critical tipping points. This has important implications for the potential response of these ecosystems to global climate change. For the boreal region, four distinct ecosystem states (i.e., treeless, sparse and dense woodland, and boreal forest) were previously identified by using the Collection 3 data of MODIS Vegetation Continuous Fields (VCF). Since then, the MODIS VCF product has been updated to Collection 5; and a Landsat VCF product of global tree cover at a fine spatial resolution of 30 meters has been developed. Here we compare these different remote-sensing products of tree cover to show that identification of alternative stable states in the boreal biome partly depends on the data source used. The updated MODIS data and the newer Landsat data consistently demonstrate three distinct modes around similar tree-cover values. Our analysis suggests that the boreal region has three modes: one sparsely vegetated state (treeless), one distinct ‘savanna-like’ state and one forest state, which could be alternative stable states. Our analysis illustrates that qualitative outcomes of studies may change fundamentally as new versions of remote sensing products are used. Scientific reproducibility thus requires that old versions remain publicly available. PMID:26571014

  6. Marginally trapped submanifolds in Lorentzian space forms and in the Lorentzian product of a space form by the real line

    SciTech Connect

    Anciaux, Henri; Godoy, Yamile

    2015-02-15

    We give local, explicit representation formulas for n-dimensional spacelike submanifolds which are marginally trapped in the Minkowski space ℝ{sub 1}{sup n+2}, the de Sitter space dS{sup n+2}, the anti-de Sitter space AdS{sup n+2} and the Lorentzian products S{sup n+1} × ℝ and ℍ{sup n+1} × ℝ of the sphere and the hyperbolic space by the real line.

  7. Methyl Bromide alternatives for vegetable production in Georgia: On-farm trials

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Three fumigant alternatives, methyl iodide plus chloropicirn (MIDAS), dimethyl disulfide plus chloropicrin (DMDS), and Telone II plus chloropicrin plus Vapam (3-Way) have exhibited promising results in small plot trials for the control of pests (weeds, pathogens, nematodes) in plasticulture producti...

  8. Alternatives to antibiotics as growth promoters for use in swine production: a review

    PubMed Central

    2013-01-01

    In the past two decades, an intensive amount of research has been focused on the development of alternatives to antibiotics to maintain swine health and performance. The most widely researched alternatives include probiotics, prebiotics, acidifiers, plant extracts and neutraceuticals such as copper and zinc. Since these additives have been more than adequately covered in previous reviews, the focus of this review will be on less traditional alternatives. The potential of antimicrobial peptides, clay minerals, egg yolk antibodies, essential oils, eucalyptus oil-medium chain fatty acids, rare earth elements and recombinant enzymes are discussed. Based on a thorough review of the literature, it is evident that a long and growing list of compounds exist which have been tested for their ability to replace antibiotics as feed additives in diets fed to swine. Unfortunately, the vast majority of these compounds produce inconsistent results and rarely equal antibiotics in their effectiveness. Therefore, it would appear that research is still needed in this area and that the perfect alternative to antibiotics does not yet exist. PMID:24034214

  9. Alternatives to antibiotics as growth promoters for use in swine production: a review.

    PubMed

    Thacker, Philip A

    2013-01-01

    In the past two decades, an intensive amount of research has been focused on the development of alternatives to antibiotics to maintain swine health and performance. The most widely researched alternatives include probiotics, prebiotics, acidifiers, plant extracts and neutraceuticals such as copper and zinc. Since these additives have been more than adequately covered in previous reviews, the focus of this review will be on less traditional alternatives. The potential of antimicrobial peptides, clay minerals, egg yolk antibodies, essential oils, eucalyptus oil-medium chain fatty acids, rare earth elements and recombinant enzymes are discussed. Based on a thorough review of the literature, it is evident that a long and growing list of compounds exist which have been tested for their ability to replace antibiotics as feed additives in diets fed to swine. Unfortunately, the vast majority of these compounds produce inconsistent results and rarely equal antibiotics in their effectiveness. Therefore, it would appear that research is still needed in this area and that the perfect alternative to antibiotics does not yet exist. PMID:24034214

  10. EVALUATION OF CURRENTLY AVAILABLE ALTERNATIVES TO METHYL BROMIDE FOR ORNAMENTAL CROP PRODUCTION IN FLORIDA

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Studies were designed to test the efficacy of the chemical alternatives, Midas™ (iodomethane:chloropicrin (pic) 50:50 [MI 50:50] and 98:2 [MI 98:2], Arysta LifeScience Corp., Cary, NC) and dimethyl disulfide:pic (Paladin™ 79:21 [DMDS], United Phosphorous, Inc., King of Prussia, PA) compared with met...

  11. MODIS NDVI Change Detection Techniques and Products Used in the Near Real Time Forwarn System for Detecting, Monitoring, and Analyzing Regional Forest Disturbances

    NASA Astrophysics Data System (ADS)

    Spruce, J.; Hargrove, W. W.; Gasser, J.; Smoot, J.; Kuper, P.

    2014-12-01

    This presentation discusses MODIS NDVI change detection methods and products used in the ForWarn Early Warning System (EWS) for near real time (NRT) recognition and tracking of regionally evident forest disturbances throughout the conterminous US (CONUS). This EWS has provided NRT forest change products to the forest health protection community since 2010, using temporally processed MODIS Aqua and Terra NDVI time series data to currently compute and post 6 different forest change products for CONUS every 8 days. Multiple change products are required to improve detectability and to more fully assess the nature of apparent disturbances. Each type of forest change product reports per pixel percent change in NDVI for a given 24 day interval, comparing current versus a given historical baseline NDVI. EMODIS 7 day expedited and MODIS MOD13 data are used to obtain current and historical NDVIs, respectively. Historical NDVI data is processed with the Time Series Product Tool (TSPT) and the Phenological Parameters Estimation Tool (PPET) software. While each change product employs maximum value compositing (MVC) of NDVI, the design of specific products primarily differs in terms of the historical baseline. The three main change products use either 1, 3, or all previous years of MVC NDVI as a baseline. Another product uses an Adaptive Length Compositing (ALC) version of MVC to derive an alternative current NDVI that is the freshest quality NDVI as opposed to merely the MVC NDVI across a 24 day time frame. The ALC approach can improve detection speed by 8 to 16 days. ForWarn also includes 2 change products that improve detectability of forest disturbances in lieu of climatic fluctuations, especially in the spring and fall. One compares current MVC NDVI to the zonal maximum under the curve NDVI per pheno-region cluster class, considering all previous years in the MODIS record. The other compares current maximum NDVI to the mean of maximum NDVI for all previous MODIS years. The

  12. A comparative human health risk assessment of p-dichlorobenzene-based toilet rimblock products versus fragrance/surfactant-based alternatives.

    PubMed

    Aronson, Dallas B; Bosch, Stephen; Gray, D Anthony; Howard, Philip H; Guiney, Patrick D

    2007-10-01

    A comparison of the human health risk to consumers using one of two types of toilet rimblock products, either a p-dichlorobenzene-based rimblock or two newer fragrance/surfactant-based alternatives, was conducted. Rimblock products are designed for global use by consumers worldwide and function by releasing volatile compounds into indoor air with subsequent exposure presumed to be mainly by inhalation of indoor air. Using the THERdbASE exposure model and experimentally determined emission data, indoor air concentrations and daily intake values were determined for both types of rimblock products. Modeled exposure concentrations from a representative p-dichlorobenzene rimblock product are an order of magnitude higher than those from the alternative rimblock products due to its nearly pure composition and high sublimation rate. Lifetime exposure to p-dichlorobenzene or the subset of fragrance components with available RfD values is not expected to lead to non-cancer-based adverse health effects based on the exposure concentrations estimated using the THERdbASE model. A similar comparison of cancer-based effects was not possible as insufficient data were available for the fragrance components. PMID:17934948

  13. FORPS: a FORTH-based production system and its application to a real-time robot control problem

    SciTech Connect

    Matheus, C.J.; Martin, H.L.

    1986-01-01

    A simple yet very powerful system has been developed that merges the artificial intelligence qualities of a production system with the real-time control capabilities of FORTH. FORPS (FORTH-based Production System) offers the advantages of intelligent, rule-based control in a small package offering high speed, extensibility, and simplicity. A practical example of the system is presented in the development of an obstacle avoidance program to aid in controlling an overhead manipulator transport system. Several other potential applications to the area of control are discussed.

  14. An alternative discourse of productive aging: A self-restrained approach in older Chinese people in Hong Kong.

    PubMed

    Luo, Minxia; Chui, Ernest Wing-Tak

    2016-08-01

    While Western discourses regarding productive aging emphasize individuals' contributions to economic productivity, the Confucian cultural heritage of the Chinese community may provide an alternative perspective. This qualitative study explores interpretations of what constitutes productive aging, based on a series of in-depth interviews with older Chinese people in Hong Kong. It shows that some of these individuals adopted a passive and indirect interpretation of productive aging, distinct from that found in Western countries. The Confucianism-based, collectivist, normative order underpinning Hong Kong society disposed these older people to adopting a self-restrained attitude with the aim of avoiding becoming a burden to others, especially family members. Such a tendency toward self-restraint or avoidance also encompassed a compromise between ideals and reality, with the older people opting to compromise their expectations of the younger generation as a whole, their adult children in particular, in terms of respect and reciprocity. PMID:27531450

  15. Hot-spots of primary productivity: An Alternative interpretation to Conventional upwelling models

    NASA Astrophysics Data System (ADS)

    van Ruth, Paul D.; Ganf, George G.; Ward, Tim M.

    2010-12-01

    The eastern Great Australian Bight (EGAB) forms part of the Southern and Indian Oceans and is an area of high ecological and economic importance. Although it supports a commercial fishery, quantitative estimates of the primary productivity underlying this industry are open to debate. Estimates range from <100 mg C m -2 day -1 to > 500 mg C m -2 day -1. Part of this variation may be due to the unique upwelling circulation of shelf waters in summer/autumn (November-April), which shares some similarities with highly productive eastern boundary current upwelling systems, but differs due to the influence of a northern boundary current, the Flinders current, and a wide continental shelf. This study examines spatial variations in primary productivity in the EGAB during the upwelling seasons of 2005 and 2006. Daily integral productivity calculated using the vertically generalised production model (VGPM) showed a high degree of spatial variation. Productivity was low (<800 mg C m -2 day -1) in offshore central and western regions of the EGAB. High productivities (1600-3900 mg C m -2 day -1) were restricted to hotspots in the east that were influenced by the upwelled water mass. There was a strong correlation between the depth of the euphotic zone and the depth of the mixed layer that suggested that ˜50% of the euphotic zone lay below the mixed layer depth. As a result, high rates of primary productivity did not require upwelled water to reach the surface. A significant proportion of total productivity in the euphotic zone (57% in 2005 and 65% in 2006) occurred in the upwelled water mass below the surface mixed layer. This result has implications for daily integral productivities modelled with the VGPM, which uses surface measures of phytoplankton biomass to calculate productivity. Macro-nutrient concentrations could not be used to explain the difference in the low and high productivities (silica > 1 μmol L -1, nitrate/nitrite > 0.4 μmol L -1, phosphate > 0.1 μmol L -1

  16. Alternative-fuel production facility for City of Huntsville, Alabama. Volume I. Executive summary

    SciTech Connect

    Not Available

    1982-10-01

    The feasibility of a Municipal Solid Waste (MSW)-to-energy project in the Huntsville area was invetigated and found to be an environmentally attractive and cost effective method of MSW disposal. Of nine likely alternatives, that were developed for detailed evaluation and analysis. Alternative 1M surfaced as best for the City. It is described as follows: a MSW-To-Energy Plant, consisting of two 300 TPD Mass Burning Waterwall Furnace/Boiler Systems at Lowe Industrial Park to provide low pressure steam to industries in the park. A MSW Transfer Station at the existing landfill and MSW as well as residue transport rolling stock equipment is included to facilitate movement of the waste materials between the two locations that are some twelve miles apart.

  17. Assessment of energy crops alternative to maize for biogas production in the Greater Region.

    PubMed

    Mayer, Frédéric; Gerin, Patrick A; Noo, Anaïs; Lemaigre, Sébastien; Stilmant, Didier; Schmit, Thomas; Leclech, Nathael; Ruelle, Luc; Gennen, Jerome; von Francken-Welz, Herbert; Foucart, Guy; Flammang, Jos; Weyland, Marc; Delfosse, Philippe

    2014-08-01

    The biomethane yield of various energy crops, selected among potential alternatives to maize in the Greater Region, was assessed. The biomass yield, the volatile solids (VS) content and the biochemical methane potential (BMP) were measured to calculate the biomethane yield per hectare of all plant species. For all species, the dry matter biomass yield and the VS content were the main factors that influence, respectively, the biomethane yield and the BMP. Both values were predicted with good accuracy by linear regressions using the biomass yield and the VS as independent variable. The perennial crop miscanthus appeared to be the most promising alternative to maize when harvested as green matter in autumn and ensiled. Miscanthus reached a biomethane yield of 5.5 ± 1 × 10(3)m(3)ha(-1) during the second year after the establishment, as compared to 5.3 ± 1 × 10(3)m(3)ha(-1) for maize under similar crop conditions. PMID:24929279

  18. A Note on a Family of Alternating Sums of Products of Binomial Numbers

    ERIC Educational Resources Information Center

    Gauthier, N.

    2013-01-01

    We study the following family of integral-valued alternating sums, where -infinity equal to or less than m equal to or less than infinity and n equal to or greater than 0 are integers [equation omitted]. We first consider h[subscript m](n) for m and n non-negative integers and show that it is of the form 2[superscript n + 2m] - P[subscript m](n),…

  19. The Real-Valued Sparse Direction of Arrival (DOA) Estimation Based on the Khatri-Rao Product

    PubMed Central

    Chen, Tao; Wu, Huanxin; Zhao, Zhongkai

    2016-01-01

    There is a problem that complex operation which leads to a heavy calculation burden is required when the direction of arrival (DOA) of a sparse signal is estimated by using the array covariance matrix. The solution of the multiple measurement vectors (MMV) model is difficult. In this paper, a real-valued sparse DOA estimation algorithm based on the Khatri-Rao (KR) product called the L1-RVSKR is proposed. The proposed algorithm is based on the sparse representation of the array covariance matrix. The array covariance matrix is transformed to a real-valued matrix via a unitary transformation so that a real-valued sparse model is achieved. The real-valued sparse model is vectorized for transforming to a single measurement vector (SMV) model, and a new virtual overcomplete dictionary is constructed according to the KR product’s property. Finally, the sparse DOA estimation is solved by utilizing the idea of a sparse representation of array covariance vectors (SRACV). The simulation results demonstrate the superior performance and the low computational complexity of the proposed algorithm. PMID:27187409

  20. The Use of OMPS Near Real Time Products in Volcanic Cloud Risk Mitigation and Smoke/Dust Air Quality Assessments

    NASA Astrophysics Data System (ADS)

    Seftor, C. J.; Krotkov, N. A.; McPeters, R. D.; Li, J. Y.; Durbin, P. B.

    2015-12-01

    Near real time (NRT) SO2 and aerosol index (AI) imagery from Aura's Ozone Monitoring Instrument (OMI) has proven invaluable in mitigating the risk posed to air traffic by SO2 and ash clouds from volcanic eruptions. The OMI products, generated as part of NASA's Land, Atmosphere Near real-time Capability for EOS (LANCE) NRT system and available through LANCE and both NOAA's NESDIS and ESA's Support to Aviation Control Service (SACS) portals, are used to monitor the current location of volcanic clouds and to provide input into Volcanic Ash (VA) advisory forecasts. NRT products have recently been developed using data from the Ozone Mapping and Profiler Suite onboard the Suomi NPP platform; they are currently being made available through the SACS portal and will shortly be incorporated into the LANCE NRT system. We will show examples of the use of OMPS NRT SO2 and AI imagery to monitor recent volcanic eruption events. We will also demonstrate the usefulness of OMPS AI imagery to detect and track dust storms and smoke from fires, and how this information can be used to forecast their impact on air quality in areas far removed from their source. Finally, we will show SO2 and AI imagery generated from our OMPS Direct Broadcast data to highlight the capability of our real time system.

  1. Accuracy of Single Frequency GPS Observations Processing In Near Real-time With Use of Code Predicted Products

    NASA Astrophysics Data System (ADS)

    Wielgosz, P. A.

    In this year, the system of active geodetic GPS permanent stations is going to be estab- lished in Poland. This system should provide GPS observations for a wide spectrum of users, especially it will be a great opportunity for surveyors. Many of surveyors still use cheaper, single frequency receivers. This paper focuses on processing of single frequency GPS observations only. During processing of such observations the iono- sphere plays an important role, so we concentrated on the influence of the ionosphere on the positional coordinates. Twenty consecutive days of GPS data from 2001 year were processed to analyze the accuracy of a derived three-dimensional relative vec- tor position between GPS stations. Observations from two Polish EPN/IGS stations: BOGO and JOZE were used. In addition to, a new test station - IGIK was created. In this paper, the results of single frequency GPS observations processing in near real- time are presented. Baselines of 15, 27 and 42 kilometers and sessions of 1, 2, 3, 4, and 6 hours long were processed. While processing we used CODE (Centre for Orbit De- termination in Europe, Bern, Switzerland) predicted products: orbits and ionosphere info. These products are available in real-time and enable near real-time processing. Software Bernese v. 4.2 for Linux and BPE (Bernese Processing Engine) mode were used. These results are shown with a reference to dual frequency weekly solution (the best solution). Obtained GPS positional time and GPS baseline length dependency accuracy is presented for single frequency GPS observations.

  2. Networking Resources, Owning Productivity: A Post-Development Alternative in Mindanao?

    ERIC Educational Resources Information Center

    Horner, Lindsey K.

    2013-01-01

    This paper explores the practices of one small non-governmental organisation (NGO) in Mindanao working innovatively to challenge power and interests by linking resources to local communities who control their productivity. While this may seem like social capital, I suggest that the agency over production, and the deeply political and ideological…

  3. An Alternate Look at Educational Psychologist's Productivity from 1991 to 2002

    ERIC Educational Resources Information Center

    Hsieh, Pei-Hsuan; Acee, Taylor; Chung, Wen-Hung; Hsieh, Ya-Ping; Kim, Hyunjin; Thomas, Greg D.; You, Ji-in; Robinson, Daniel H.

    2004-01-01

    Previous investigations of the productivity of educational psychologists (Smith et al., 1998 and Smith et al., 2003) have used a points system that defines high productivity as having few co-authors and high authorship placement. Due to the increasingly collaborative nature of educational psychology research (Robinson, McKay, Katayama, & Fan,…

  4. Coal gasification systems engineering and analysis. Appendix C: Alternate product facility designs

    NASA Technical Reports Server (NTRS)

    1980-01-01

    The study of the production of methane, methanol, gasoline, and hydrogen by an add-on facility to a Koppers-Totzek based MBG plant is presented. Applications to a Texaco facility are inferred by evaluation of delta effects from the K-T cases. The production of methane from an add-on facility to a Lurgi based MBG plant and the co-production of methane and methanol from a Lurgi based system is studied. Studies are included of the production of methane from up to 50 percent of the MBG produced in an integrated K-T based plant and the production of methane from up to 50 percent of the MBG produced from an integrated plant in which module 1 is based on K-T technology and modules 2, 3, and 4 are based on Texaco technology.

  5. Evaluation of the combination of dimethyl disulfide and dazomet as an efficient methyl bromide alternative for cucumber production in China.

    PubMed

    Mao, Liangang; Yan, Dongdong; Wang, Qiuxia; Li, Yuan; Ouyang, Canbin; Liu, Pengfei; Shen, Jin; Guo, Meixia; Cao, Aocheng

    2014-05-28

    The combination of dimethyl disulfide (DMDS) and dazomet (DZ) is a potential alternative to methyl bromide (MB) for soil disinfestation. The efficacy of DMDS plus DZ in controlling key soilborne pests was evaluated in a laboratory study and in two commercial cucumber greenhouses. Laboratory studies found that all of the combinations had positive synergistic effects on root-knot nematodes, two key soilborne fungi, and two major weed seeds. Greenhouse trials revealed that the combination of DMDS and DZ (30 + 25 g m(-2)) successfully suppressed Meloidogyne spp. root galling, sharply reduced the colony-forming units of Fusarium spp. and Phytophthora spp. on media, maintained high cucumber yields, and was not significantly different from MB or DMDS alone, but better than DZ alone. All of the chemical treatments provided significantly better results than the nontreated control. The results indicate that the combination of DMDS and DZ is an efficient MB alternative for cucumber production. PMID:24820184

  6. Detection of adulterated murine components in meat products by TaqMan© real-time PCR.

    PubMed

    Fang, Xin; Zhang, Chi

    2016-02-01

    Using murine meat to substitute mutton has been identified as a new type of meat fraud in China, yet no detection method for murine species has been reported. Here, three kinds of rodent were used as target species to establish a murine-specific real-time PCR method of detection. The mitochondrial cytochrome b gene (cytb) of each target was sequenced and a TaqMan probe was designed based on the cytb. Simultaneously, an internal positive control (IPC) plasmid along with its respective probe were designed to monitor the PCR reaction. As a result, the duplex real-time PCR system was verified to be specific. The limit of detection (LOD) was lower than 1 pg of DNA per reaction and 0.1% murine contamination in meat mixtures. Standard curves were generated for a quantitative analysis. Thus, this study provided a new tool to control the quality of meat products for official and third-party laboratories. PMID:26304376

  7. New In Situ Capture Quantitative (Real-Time) Reverse Transcription-PCR Method as an Alternative Approach for Determining Inactivation of Tulane Virus

    PubMed Central

    Wang, Dapeng; Xu, Shuxia; Yang, David; Young, Glenn M.

    2014-01-01

    Human noroviruses (HuNoVs) are the major cause of epidemic nonbacterial gastroenteritis. Although quantitative (real-time) reverse transcription-PCR (qRT-PCR) is widely used for detecting HuNoVs, it only detects the presence of viral RNA and does not indicate viral infectivity. Human blood group antigens (HBGAs) have been identified as receptors/co-receptors for both HuNoVs and Tulane virus (TV) and are crucial for viral infection. We propose that viral infectivity can be evaluated with a molecular assay based on receptor-captured viruses. In this study, we employed TV as an HuNoV surrogate to validate the HBGA-based capture qRT-PCR method against the 50% tissue culture infectious dose (TCID50) method. We employed type B HBGA on an immuno-well module to concentrate TV, followed by amplification of the captured viral genome by in situ qRT-PCR. We first demonstrated that this in situ capture qRT-PCR (ISC-qRT-PCR) method could effectively concentrate and detect TV. We then treated TV under either partial or full inactivation conditions and measured the remaining infectivity by ISC-qRT-PCR and a tissue culture-based amplification method (TCID50). We found that the ISC-qRT-PCR method could be used to evaluate virus inactivation deriving from damage to the capsid and study interactions between the capsid and viral receptor. Heat, chlorine, and ethanol treatment primarily affect the capsid structure, which in turns affects the ability of the capsid to bind to viral receptors. Inactivation of the virus by these methods could be reflected by the ISC-qRT-PCR method and confirmed by TCID50 assay. However, the loss of the infectivity caused by damage to the viral genome (such as that from UV irradiation) could not be effectively reflected by this method. Despite this limitation, the ISC-qRT-PCR provides an alternative approach to determine inactivation of Tulane virus. A particular advantage of the ISC-qRT-PCR method is that it is also a faster and easier method to effectively

  8. Direct analysis in real time mass spectrometry of potential by-products from homemade nitrate ester explosive synthesis.

    PubMed

    Sisco, Edward; Forbes, Thomas P

    2016-04-01

    This work demonstrates the coupling of direct analysis in real time (DART) ionization with time-of-flight mass spectrometry (MS) in an off-axis configuration for the trace detection and analysis of potential partially nitrated and dimerized by-products of homemade nitrate ester explosive synthesis. Five compounds relating to the synthesis of nitroglycerin (NG) and pentaerythritol tetranitrate (PETN) were examined. Deprotonated ions and adducts with molecular oxygen, nitrite, and nitrate were observed in the mass spectral responses of these compounds. A global optimum temperature of 350 °C for the by-products investigated here enabled single nanogram to sub nanogram trace detection. Matrix effects were examined through a series of mixtures containing one or more compounds (sugar alcohol precursors, by-products, and/or explosives) across a range of mass loadings. The explosives MS responses experienced competitive ionization in the presence of all by-products. The magnitude of this influence corresponded to both the degree of by-product nitration and the relative mass loading of the by-product to the explosive. This work provides a characterization of potential by-products from homemade nitrate ester synthesis, including matrix effects and potential challenges that might arise from the trace detection of homemade explosives (HMEs) containing impurities. Detection and understanding of HME impurities and complex mixtures may provide valuable information for the screening and sourcing of homemade nitrate ester explosives. PMID:26838397

  9. Real encoded genetic algorithm and response surface methodology to optimize production of an indolizidine alkaloid, swainsonine, from Metarhizium anisopliae.

    PubMed

    Singh, Digar; Kaur, Gurvinder

    2013-09-01

    Response surface methodology (RSM) and artificial neural network-real encoded genetic algorithm (ANN-REGA) were employed to develop a process for fermentative swainsonine production from Metarhizium anisopliae (ARSEF 1724). The effect of finally screened process variables viz. inoculum size, oatmeal extract, glucose, and CaCl2 were investigated through central composite design and were further utilized for training sets in ANN with training and test R values of 0.99 and 0.94, respectively. ANN-REGA was finally employed to simulate the predictive swainsonine production with best evolved media composition. ANN-REGA predicted a more precise fermentation model with 103 % (shake flask) increase in alkaloid production compared to 75.62 % (shake flask) obtained with RSM model upon validation. PMID:23315485

  10. Influence of productivity on the stability of real and model ecosystems

    SciTech Connect

    Moore, J.C. ); Ruiter, P.C. de ); Hunt, H.W. )

    1993-08-13

    The lengths of food chains within ecosystems have been thought to be limited either by the productivity of the ecosystem or by the resilience of that ecosystem after perturbation. Models based on ecological energetics that follow the form of Lotka-Volterra equations and equations that include material (detritus) recycling show that productivity and resilience are inextricably interrelated. The models were initialized with data from 5- to 10-year studies of actual soil food webs. Estimates indicate that most ecological production worldwide is from ecosystems that are themselves sufficiently productive to recover from minor perturbations.

  11. Semi-solid-state fermentation: a promising alternative for neomycin production by the actinomycete Streptomyces fradiae.

    PubMed

    Machado, Isabel; Teixeira, José A; Rodríguez-Couto, Susana

    2013-06-10

    The production of neomycin by the actinomycete Streptomyces fradiae, under semi-solid-state fermentation conditions was the main subject of this study. Two supports (nylon sponge and orange peelings) were tested in order to determine the most suitable one for the production of neomycin by the above-mentioned microorganism. Nylon sponge led to the highest neomycin production, reaching a maximum value of 13,903 μg/mL on the 10th day of cultivation. As a control, the same experiment was performed under submerged fermentation (SmF) conditions, without solid support. Here the production of neomycin by S. fradiae was about 55-fold lower (i.e. 250 μg/mL) than that obtained for SSF. PMID:23570968

  12. Crop productivity and economics during the transition to alternative cropping systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Increasing economic pressures and continued environmental concerns in agricultural production have heightened the need for more sustainable cropping systems. Research is needed to identify systems that simultaneously improve the economic and social viability of farms and rural communities while prot...

  13. Alternative methods of processing bio-feedstocks in formulated consumer product design

    PubMed Central

    Peremezhney, Nicolai; Jacob, Philipp-Maximilian; Lapkin, Alexei

    2014-01-01

    In this work new methods of processing bio-feedstocks in the formulated consumer products industry are discussed. Our current approach to formulated products design is based on heuristic knowledge of formulators that allows selecting individual compounds from a library of available materials with known properties. We speculate that most of the compounds (or functions) that make up the product to be designed can potentially be obtained from a few bio-sources. In this case, it may be possible to design a sequence of transformations required to convert feedstocks into products with desired properties, analogous to a metabolic pathway of a complex organism. We conceptualize some novel approaches to processing bio-feedstocks with the aim of bypassing the step of a fixed library of ingredients. Two approaches are brought forward: one making use of knowledge-based expert systems and the other making use of applications of metabolic engineering and dynamic combinatorial chemistry. PMID:24860803

  14. Alternative methods of processing bio-feedstocks in formulated consumer product design

    NASA Astrophysics Data System (ADS)

    Peremezhney, Nicolai; Jacob, Philipp-Maximilian; Lapkin, Alexei

    2014-05-01

    In this work new methods of processing bio-feedstocks in the formulated consumer products industry are discussed. Our current approach to formulated products design is based on heuristic knowledge of formulators that allows selecting individual compounds from a library of available materials with known properties. We speculate that most of the compounds (or functions) that make up the product to be designed can potentially be obtained from a few bio-sources. In this case, it may be possible to design a sequence of transformations required to convert feedstocks into products with desired properties, analogous to a metabolic pathway of a complex organism. We conceptualize some novel approaches to processing bio-feedstocks with the aim of bypassing the step of a fixed library of ingredients. Two approaches are brought forward: one making use of knowledge-based expert systems and the other making use of applications of metabolic engineering and dynamic combinatorial chemistry.

  15. Fusion of product and process data: Batch-mode and real-time streaming

    SciTech Connect

    Vincent De Sapio; Spike Leonard

    1999-12-01

    In today's DP product realization enterprise it is imperative to reduce the design-to-fabrication cycle time and cost while improving the quality of DP parts (reducing defects). Much of this challenge resides in the inherent gap between the product and process worlds. The lack of seamless, bi-directional flow of information prevents true concurrency in the product realization world. This report addresses a framework for product-process data fusion to help achieve next generation product realization. A fundamental objective is to create an open environment for multichannel observation of process date, and subsequent mapping of that data onto product geometry. In addition to the sensor-based observation of manufacturing processes, model-based process data provides an important complement to empirically acquired data. Two basic groups of manufacturing models are process physics, and machine kinematics and dynamics. Process physics addresses analytical models that describe the physical phenomena of the process itself. Machine kinematic and dynamic models address the mechanical behavior of the processing equipment. As a secondary objective, an attempt has been made in this report to address part of the model-based realm through the development of an open object-oriented library and toolkit for machine kinematics and dynamics. Ultimately, it is desirable to integrate design definition, with all types of process data; both sensor-based and model-based. Collectively, the goal is to allow all disciplines within the product realization enterprise to have a centralized medium for the fusion of product and process data.

  16. Precipitation Analysis at Fine Time Scales using TRMM and Other Satellites: Real-time and Research Products and Applications

    NASA Technical Reports Server (NTRS)

    Adler, Robert; Huffman, George; Bolvin, David; Nelkin, Eric; Curtis, Scott; Pierce, Harold; Gu, Guo-Jon

    2004-01-01

    Quasi-global precipitation analyses at fine time scales (3-hr) are described. TRMM observations (radar and passive microwave) are used to calibrate polar-orbit microwave observations from SSM/I (and other satellites instruments, including AMSR and AMSU) and geosynchronous IR observations. The individual data sets are then merged using a priority order based on quality to form the TRMM Multi-satellite Precipitation Analysis (MPA). Raingauge information is used to help constrain the satellite-based estimates over land. The TRMM standard research product (Version 6 3B-42 of the TRMM products) will be available for the entire TRMM period (January 1998-present) by the end of 2004. The real-time version of this merged product has been produced over the past two years and is available on the U.S. TRMM web site (trmm.gsfc.nasa.gov) at 0.25" latitude-longitude resolution over the latitude range from 5O0N-50"S. Validation of daily totals indicates good results, with limitations noted in mid-latitude winter over land and regions of shallow, orographic precipitation. Various applications of these estimates are described, includmg: 1) detecting potential floods in near real-time; 2) analyzing Indian Ocean precipitation variations related to the initiation of El Nino; 3) determining characteristics of the African monsoon; and 4) analysis of diurnal variations.

  17. Precipitation Analysis at Fine Time Scales Using Multiple Satellites: Real-time and Research Products and Applications

    NASA Technical Reports Server (NTRS)

    Adler, Robert; Huffman, George; Bolvin, David; Nelkin, Eric; Curtis, Scott; Pierce, Harold

    2004-01-01

    Quasi-global precipitation analyses at fine time scales (3-hr) are described. TRMM observations (radar and passive microwave) are used to calibrate polar-orbit microwave observations from SSM/I (and other satellites instruments, including AMSR and AMSU) and geosynchronous IR observations. The individual data sets are then merged using a priority order based on quality to form the Multi-satellite Precipitation Analysis (MPA). Raingauge information is used to help constrain the satellite-based estimates over land. The TRMM standard research product (Version 6 3B-42 of the TRMM products) will be available for the entire TRMM period (January 1998-present) in 2004. The real-time version of this merged product has been produced over the past two years and is available on the U.S. TRMM web site (trmm.gsfc.nasa.gov) at 0.25" latitude-longitude resolution over the latitude range from 5O"N-5O0S. Validation of daily totals indicates good results, with limitations noted in mid-latitude winter over land and regions of shallow, orographic precipitation. Various applications of these estimates are described, including: 1) detecting potential floods in near real-time; 2) analyzing Indian Ocean precipitation variations related to the initiation of El Nino; 3) determining characteristics of the African monsoon; and 4) analysis of diurnal variations.

  18. The multi-year cumulative effects of alternative stocking rate and grazing management practices on pasture productivity and utilization efficiency.

    PubMed

    McCarthy, B; Delaby, L; Pierce, K M; McCarthy, J; Fleming, C; Brennan, A; Horan, B

    2016-05-01

    The production and utilization of increased quantities of high quality pasture is of paramount importance in pasture-based milk production systems. The objective of this study was to evaluate the cumulative effects of alternative integrated grazing strategies, incorporating alternative stocking rate (SR) and grazing severities, on pasture productivity and grazing efficiency over multiple years within farm systems using perennial ryegrass dominant pastures. Three whole-farm SR treatments were compared over 4 complete grazing seasons (2009 to 2012 inclusive): low (2.51 cows/ha; LSR), medium (2.92 cows/ha; MSR), and high (3.28 cows/ha; HSR). Each system had its own farmlet containing 18 paddocks and remained on the same treatment for the duration of the study. Stocking rate had a significant effect on all grazing variables with the exception of soil fertility status and sward density. Increased SR resulted in increased total annual net pasture accumulation, improved sward nutritive value, and increased grazed pasture utilization. Total annual net pasture accumulation was greatest in HSR [15,410kg of dry matter (DM)/ha], intermediate for MSR (14,992kg of DM/ha), and least for LSR (14,479kg of DM/ha) during the 4-yr study period. A linear effect of SR on net pasture accumulation was detected with an increase in net pasture accumulation of 1,164.4 (SE=432.7) kg of DM/ha for each 1 cow/ha increase in SR. Pregrazing pasture mass and height and postgrazing residual pasture mass and height were greatest for LSR, intermediate for the MSR, and lowest for the HSR. In comparison with the LSR, the imposition of a consistently increased grazing severity coupled with increased whole farm SR in MSR and HSR treatments arrested the decline in sward nutritive value, typically observed during mid-season. Incorporating the individual beneficial effects of SR on pasture accumulation, nutritive value, and utilization efficiency, total proportional energy (unité fourragère lait

  19. The Primary and Secondary Production of Germanium: A Life-Cycle Assessment of Different Process Alternatives

    NASA Astrophysics Data System (ADS)

    Robertz, Benedicte; Verhelle, Jensen; Schurmans, Maarten

    2015-02-01

    Germanium is a semiconducting metalloid element used in optical fibers, catalysis, infrared optics, solar cells, and light-emitting diodes. The need for Ge in these markets is considered to increase by a steady ~1% on a yearly basis. Its economic importance, coupled with the identified supply risks, has led to the classification of germanium as a critical raw material within Europe. Since the early 1950s, Umicore Electro-Optic Materials has supplied germanium-based materials solutions to its markets around the world. Umicore extracts germanium from a wide range of refining and recycling feeds. The main objectives of this study were to quantify the potential environmental impacts of the production of germanium from production scraps from the photovoltaic industry and to compare them with the potential impacts of the primary production of germanium from coal. The data related to the secondary production are Umicore-specific data. Environmental impact scores have been calculated for the impact categories recommended by the International reference life cycle data system. The comparison of the primary and secondary production highlights the benefit linked to the recycling of metals.

  20. A note on a family of alternating sums of products of binomial numbers

    NASA Astrophysics Data System (ADS)

    Gauthier, N.

    2013-03-01

    We study the following family of integral-valued alternating sums, where ? and ? are integers: We first consider ? for ? and ? non-negative integers and show that it is of the form ? , where ? may be represented as a polynomial of degree ? in ? , or expressed as a non-polynomial closed form given by a sum of binomial numbers. We then consider ? for ? a negative integer and for ? a non-negative integer. This reveals, in particular, that ? for ? , that ? for ? . We also show that ? is a polynomial of degree ? in ? , for fixed ? , with ? , and we give expressions for the coefficients.

  1. Modeling the impacts of alternative fertilization methods on nitrogen loading in rice production in Shanghai.

    PubMed

    Zhao, Zheng; Sha, Zhimin; Liu, Yibo; Wu, Shuhang; Zhang, Hanlin; Li, Changsheng; Zhao, Qi; Cao, Linkui

    2016-10-01

    Nitrogen (N) loss from paddy fields is an important source of agricultural non-point source pollution that leads to eutrophication of water bodies and degradation of water quality. The impacts of alternative N fertilizer management practices on N loading (N loss through runoff and leaching) from paddy fields in Shanghai were assessed using a process-based biogeochemical model, DNDC. The results indicated that the current fertilization rate in paddy fields of Shanghai (300kgN/ha) exceeds the actual rice demand and has led to substantial N loading of 1142±276kg. The combined application of urea at 150kgN/ha and organic manure at 100kgN/ha was identified as the best fertilization method for rice cultivation in Shanghai; this application maintained optimal rice yields and significantly reduced N loading to 714±151kg in comparison with the current fertilization rate. A sensitivity test was conducted with various input parameters, and the results indicated that fertilization, precipitation and soil properties were the most sensitive factors that regulate N loss from paddy fields. The variability of soil properties, especially SOC led to high uncertainties in the simulated results. Therefore, the local climate conditions and soil properties should be taken into account in the identification of the best management practice (BMP) for rice cultivation, given the high spatially heterogeneous N loading values across all towns used in the simulation. The DNDC model is an effective approach for simulating and predicting N loading in paddy fields under alternative agricultural management practices. PMID:27317135

  2. The effect of complimentary and alternative medicine products on laboratory testing.

    PubMed

    Rosecrans, Robert; Dohnal, James C

    2009-02-01

    A multi-billion dollar industry has evolved over the last decade based on herbal product sales with an underlying belief that herbals are natural and therefore safe. The herbal product industry is essentially unregulated and producers are not required to follow good manufacturing practices (GMP). Batch to batch product variation, heavy metal and pesticide contamination, and even therapeutic drug contamination are problematic. Compounding these manufacturing issues are drug to drug and drug to herbal interactions that can cause cytochrome induction or inhibition. It is important for physicians to query their patients on herbal use and educate them on the potential adverse reactions. Herbals have been used for thousands of years and undoubtedly have demonstrated health benefits. However, more research is needed to gain an understanding of the complexity issues from mechanism of action to interference with clinical laboratory testing. PMID:19292027

  3. Fruit production of Attalea colenda (Arecaceae) in coastal Ecuador - an alternative oil resource?

    SciTech Connect

    Feil, J.P.

    1996-07-01

    Attalea colenda is a monoecious palm found in pastures in coastal Ecuador. In dry regions, it is a valuable source of oil in self-sufficiency farming or in combination with cattle in pastures. The palm was studied over a gradient of dry to humid environments during two fruiting seasons. Palm growth, production of leaves, inflorescences, and infructescences, number of fruits per infructescence, and seed weight of five populations were evaluated. The individual of average size is 15 m tall, which corresponds to approximately 30-40 years of age. No difference in fruit production was recorded between wet and dry regions of coastal Ecuador. The average production of one hectare of pasture, with 50 palms, was 0.9 t of oil per year. One population that was part of an agroforestry system produced 50% more fruits than the average of all populations in pasture. 18 refs., 1 fig., 6 tabs.

  4. Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory

    PubMed Central

    Kuiken, Todd; Vejerano, Eric P; McGinnis, Sean P; Hochella, Michael F; Rejeski, David; Hull, Matthew S

    2015-01-01

    Summary To document the marketing and distribution of nano-enabled products into the commercial marketplace, the Woodrow Wilson International Center for Scholars and the Project on Emerging Nanotechnologies created the Nanotechnology Consumer Products Inventory (CPI) in 2005. The objective of this present work is to redevelop the CPI by leading a research effort to increase the usefulness and reliability of this inventory. We created eight new descriptors for consumer products, including information pertaining to the nanomaterials contained in each product. The project was motivated by the recognition that a diverse group of stakeholders from academia, industry, and state/federal government had become highly dependent on the inventory as an important resource and bellweather of the pervasiveness of nanotechnology in society. We interviewed 68 nanotechnology experts to assess key information needs. Their answers guided inventory modifications by providing a clear conceptual framework best suited for user expectations. The revised inventory was released in October 2013. It currently lists 1814 consumer products from 622 companies in 32 countries. The Health and Fitness category contains the most products (762, or 42% of the total). Silver is the most frequently used nanomaterial (435 products, or 24%); however, 49% of the products (889) included in the CPI do not provide the composition of the nanomaterial used in them. About 29% of the CPI (528 products) contain nanomaterials suspended in a variety of liquid media and dermal contact is the most likely exposure scenario from their use. The majority (1288 products, or 71%) of the products do not present enough supporting information to corroborate the claim that nanomaterials are used. The modified CPI has enabled crowdsourcing capabilities, which allow users to suggest edits to any entry and permits researchers to upload new findings ranging from human and environmental exposure data to complete life cycle

  5. Access to Real-time and Historical Satellite Products from a Mobile Application

    NASA Astrophysics Data System (ADS)

    Chee, T.; Kelly, M.; Nguyen, L.; Minnis, P.; Palikonda, R.; Spangenberg, D. A.; Ayers, J.

    2011-12-01

    The rise of mobile computing provides a novel medium for the dissemination of satellite imagery and cloud products to end users. The authors describe an application built to explore the delivery of satellite-derived cloud products to mobile devices for research purposes and other applications. This project builds upon earlier work performed at NASA Langley to find novel ways to improve the delivery of satellite data and products to end-users. Since end-user needs range from visualization to information download, the application requirements reflect expected usage and also functionality that leverages conditions present in the mobile environment.

  6. Nanotechnology in the real world: Redeveloping the nanomaterial consumer products inventory.

    PubMed

    Vance, Marina E; Kuiken, Todd; Vejerano, Eric P; McGinnis, Sean P; Hochella, Michael F; Rejeski, David; Hull, Matthew S

    2015-01-01

    To document the marketing and distribution of nano-enabled products into the commercial marketplace, the Woodrow Wilson International Center for Scholars and the Project on Emerging Nanotechnologies created the Nanotechnology Consumer Products Inventory (CPI) in 2005. The objective of this present work is to redevelop the CPI by leading a research effort to increase the usefulness and reliability of this inventory. We created eight new descriptors for consumer products, including information pertaining to the nanomaterials contained in each product. The project was motivated by the recognition that a diverse group of stakeholders from academia, industry, and state/federal government had become highly dependent on the inventory as an important resource and bellweather of the pervasiveness of nanotechnology in society. We interviewed 68 nanotechnology experts to assess key information needs. Their answers guided inventory modifications by providing a clear conceptual framework best suited for user expectations. The revised inventory was released in October 2013. It currently lists 1814 consumer products from 622 companies in 32 countries. The Health and Fitness category contains the most products (762, or 42% of the total). Silver is the most frequently used nanomaterial (435 products, or 24%); however, 49% of the products (889) included in the CPI do not provide the composition of the nanomaterial used in them. About 29% of the CPI (528 products) contain nanomaterials suspended in a variety of liquid media and dermal contact is the most likely exposure scenario from their use. The majority (1288 products, or 71%) of the products do not present enough supporting information to corroborate the claim that nanomaterials are used. The modified CPI has enabled crowdsourcing capabilities, which allow users to suggest edits to any entry and permits researchers to upload new findings ranging from human and environmental exposure data to complete life cycle assessments. There

  7. Design and performance of cooled perches for alternative egg laying production systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Heat stress in both mechanically and naturally ventilated egg production facilities is a problem for the egg industry. Various means of providing supplemental cooling to hens in facilities are available, including tunnel ventilation to increase convective losses and evaporative cooling from either p...

  8. Words as Species: An Alternative Approach to Estimating Productive Vocabulary Size

    ERIC Educational Resources Information Center

    Meara, Paul M.; Alcoy, Juan Carlos Olmos

    2010-01-01

    This paper addresses the issue of how we might be able to assess productive vocabulary size in second language learners. It discusses some previous attempts to develop measures of this sort, and argues that a fresh approach is needed in order to overcome some persistent problems that dog research in this area. The paper argues that there might be…

  9. Activated carbons from waste biomass: an alternative use for biodiesel production solid residues.

    PubMed

    Nunes, Anne A; Franca, Adriana S; Oliveira, Leandro S

    2009-03-01

    Defective coffee press cake, a residue from coffee oil biodiesel production, was evaluated as raw material for production of an adsorbent for removal of methylene blue (MB) from aqueous solution. Batch adsorption tests were performed at 25 degrees C and the effects of particle size, contact time, adsorbent dosage and pH were investigated. Preliminary adsorption tests indicated that thermal treatment is necessary in order to improve adsorption capacity. Adsorption kinetics was determined by fitting first and second-order kinetic models to the experimental data, with the second-order model providing the best description of MB adsorption onto the prepared adsorbent. The experimental adsorption equilibrium data were fitted to Langmuir, Freundlich and Temkin adsorption models, with the last two providing the best fits. The experimental data obtained in the present study indicated that this type of waste material is a suitable candidate for use in the production of adsorbents for removal of cationic dyes, thus contributing for the implementation of sustainable development in both the coffee and biodiesel production chains. PMID:18996006

  10. EFFECTS OF BAITS AND BAIT ALTERNATIVES ON SLUG MORTALITY, EGG PRODUCTION, AND SEEDLING SURVIVAL

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Two non-bait slug control formulations that are not attractive to earthworms including Durham 3.5 and 7.5 (3.5 and 7.5%, metaldehyde, respectively) and SlugFest AWF (all-weather-formula, 25%, metaldehyde), a liquid spray product were investigated for their efficacy in reducing egg fecundity and slu...

  11. Development of alternative oxygen production source using a zirconia solid electrolyte membrane

    SciTech Connect

    Suitor, J.W.; Clark, D.J.; Losey, R.W.

    1990-08-01

    The objective of this multiyear effort was the development, fabrication and testing of a zirconia oxygen production module capable of delivering approximately 100 liters/minute (LPM) of oxygen. The work discussed in this report consists of development and improvement of the zirconia cell along with manufacture of cell components, preliminary design of the final plant, additional economic analysis and industrial participation. (VC)

  12. Development of alternative oxygen production source using a zirconia solid electrolyte membrane. Final report

    SciTech Connect

    Suitor, J.W.; Clark, D.J.; Losey, R.W.

    1990-08-01

    The objective of this multiyear effort was the development, fabrication and testing of a zirconia oxygen production module capable of delivering approximately 100 liters/minute (LPM) of oxygen. The work discussed in this report consists of development and improvement of the zirconia cell along with manufacture of cell components, preliminary design of the final plant, additional economic analysis and industrial participation. (VC)

  13. Productivity, job satisfaction, and health and safety in the coal industry: the participatory alternative

    SciTech Connect

    Not Available

    1982-01-01

    This is a conference which presents results and ideas on workplace participation in the coal industry. It discusses the theory of the quality circle groups for developing their own production rates and design goals. It presents the results of different coal company participation in this idea and how to implement this option. Individual topics are entered into the Data Base as separate items.

  14. Methyl Bromide alternatives for vegetable production in Georgia: Small-plot trials

    Technology Transfer Automated Retrieval System (TEKTRAN)

    In Georgia, the loss of MeBr directly impacts the production and profitability of several fruiting vegetables [specifically, pepper (Capsicum annuum L.), eggplant (Solanum melogena L.), and tomato (Lycopersicum esculentum Mill)) and cucurbits (specifically, squash [yellow (Cucurbita pepo L.)], melon...

  15. 77 FR 5696 - Exceptions or Alternatives to Labeling Requirements for Products Held by the Strategic National...

    Federal Register 2010, 2011, 2012, 2013, 2014

    2012-02-06

    ..., approved, or cleared product labels. Certain information, such as expiration dates, warnings for users.... Background In the Federal Register of December 28, 2007 (72 FR 73589), FDA issued an interim final rule... of publication in the Federal Register. \\1\\ In the Federal Register of November 18, 2008 (73 FR...

  16. ARS Research Review on "Recent progress in developing alternative strategies to antibiotics in poultry production"

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The global animal industry needs to address the increasing regulatory restrictions on the use of antibiotic growth promoters (AGPs) in animal production. Many AGPs have already been restricted by animal farms in the European Union and soon other countries are expected to be under increasing scrutin...

  17. Developmental Dynamics of SES-Related Differences in Children's Production of Obligatory and Variable Phonological Alternations

    ERIC Educational Resources Information Center

    Chevrot, Jean-Pierre; Nardy, Aurelie; Barbu, Stephanie

    2011-01-01

    Numerous studies conducted in both the psycholinguistic and sociolinguistic fields have established that the parents' socio-economic status (SES) influences several aspects of children's language production. Moreover, a number of psycholinguistic studies strongly suggest that these differences are due in part to differences in the nature and the…

  18. Crop Response to Hybrid Poplar Alternative Soilless Substrate Component for Pacific Northwest Ornamental Container Production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Douglas fir bark (DFB) is the primary component used in soilless substrate to grow woody ornamental plants in the Pacific Northwest. Bark is a by-product of the timber industry and is used for landscape mulch, a soilless substrate component, or bio-energy. The decline in the housing market and rise ...

  19. Reduced rates and alternatives to methyl bromide for snapdragon production in Florida

    Technology Transfer Automated Retrieval System (TEKTRAN)

    A field trial was conducted to evaluate soil solarization, Midas™ (iodomethane:chloropicrin 50:50, Arysta LifeScience Corp., Cary, NC) and different rates and formulations of methyl bromide under standard and metalized films for the production of snapdragon (Antirrhinum majus) in Martin County, Flor...

  20. Development of alternative oxygen production source using a zirconia solid electrolyte membrane

    NASA Technical Reports Server (NTRS)

    Suitor, J. W.; Clark, D. J.; Losey, R. W.

    1990-01-01

    The objective of this multiyear effort was the development, fabrication and testing of a zirconia oxygen production module capable of delivering approximately 100 liters/minute (LPM) of oxygen. The work discussed in this report consists of development and improvement of the zirconia cell along with manufacture of cell components, preliminary design of the final plant, additional economic analysis and industrial participation.

  1. Investigating alternative strategies for managing bacterial angular leaf spot in strawberry nursery production

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The focus of this article is to discuss some of the approaches we have tested for managing the bacterial pathogen Xanthomonas fragariae in infected strawberry nursery stock. X. fragariae causes angular leaf spot (ALS) in strawberry. The pathogen is transmitted to production fields almost exclusively...

  2. Flood and Landslide Applications of Near Real-time Satellite Rainfall Products

    NASA Technical Reports Server (NTRS)

    Hong, Yang; Adler, Robert F.; Negri, Andrew; Huffman, George J.

    2007-01-01

    Floods and associated landslides are one of the most widespread natural hazards on Earth, responsible for tens of thousands of deaths and billions of dollars in property damage every year. During 1993-2002, over 1000 of the more than 2,900 natural disasters reported were due to floods. These floods and associated landslides claimed over 90,000 lives, affected over 1.4 billion people and cost about $210 billion. The impact of these disasters is often felt most acutely in less developed regions. In many countries around the world, satellite-based precipitation estimation may be the best source of rainfall data due to lack of surface observing networks. Satellite observations can be of essential value in improving our understanding of the occurrence of hazardous events and possibly in lessening their impact on local economies and in reducing injuries, if they can be used to create reliable warning systems in cost-effective ways. This article addressed these opportunities and challenges by describing a combination of satellite-based real-time precipitation estimation with land surface characteristics as input, with empirical and numerical models to map potential of landslides and floods. In this article, a framework to detect floods and landslides related to heavy rain events in near-real-time is proposed. Key components of the framework are: a fine resolution precipitation acquisition system; a comprehensive land surface database; a hydrological modeling component; and landslide and debris flow model components. A key precipitation input dataset for the integrated applications is the NASA TRMM-based multi-satellite precipitation estimates. This dataset provides near real-time precipitation at a spatial-temporal resolution of 3 hours and 0.25deg x 0.25deg. By careful integration of remote sensing and in-situ observations, and assimilation of these observations into hydrological and landslide/debris flow models with surface topographic information, prediction of useful

  3. Rational selection of alternative, environmentally compatible surfactants for biotechnological production of pharmaceuticals--a step toward green biotechnology.

    PubMed

    Straub, Jürg Oliver; Shearer, Russel; Studer, Martin

    2014-09-01

    The biotechnological production of pharmaceutical active substances needs ancillary substances. Surfactants are used at the end of the cell culture as a protection against potential viral or bacterial contamination and to lyse the producing cells for isolation and purification of the products. To find a replacement for a surfactant that had raised environmental concern, environmentally relevant data for potential alternatives were searched for in the literature. Significant data gaps were filled with additional tests: biodegradability, algal growth inhibition, acute daphnid immobilization and chronic daphnid reproduction toxicity, acute fish toxicity, and activated sludge respiration inhibition. The results were used to model removal in the wastewater treatment plants (WWTPs) serving 3 biotechnological production sites in the Roche Group. Predicted environmental concentrations (PECs) were calculated using realistic amounts of surfactants and site-specific wastewater fluxes, modeled removals for the WWTPs and dilution factors by the respective receiving waters. Predicted no-effect concentrations (PNECs) were derived for WWTPs and for both fresh and marine receiving waters as the treated wastewater of 1 production site is discharged into a coastal water. This resulted in a spreadsheet showing PECs, PNECs, and PEC ÷ PNEC risk characterization ratios for the WWTPs and receiving waters for all investigated surfactants and all 3 sites. This spreadsheet now serves as a selection support for the biotechnological developers. This risk-based prioritization of surfactants is a step toward green biotechnological production. PMID:24917475

  4. Multi-batch catfish production and economic analysis using alternative low-cost diets with corn gluten feed and traditional diets with meat-and-bone meal

    Technology Transfer Automated Retrieval System (TEKTRAN)

    We conducted concurrent feeding trials for one growing season with channel catfish in ponds in Mississippi and Arkansas to evaluate the production and economic effects of alternative (low-cost) feeds containing 28 or 32% protein and alternative (corn gluten feed) or traditional (porcine meat, bone a...

  5. Exon recognition and nucleocytoplasmic partitioning determine AMPD1 alternative transcript production.

    PubMed Central

    Mineo, I; Holmes, E W

    1991-01-01

    Two mature transcripts are produced from the rat AMP deaminase 1 (AMPD1) gene, one that retains exon 2 and one from which exon 2 has been removed. The ratio of these two transcripts is controlled by stage-specific and tissue-specific signals (I. Mineo, P. R. H. Clarke, R. L. Sabina, and E. W. Holmes, Mol. Cell. Biol. 10:5271-5278, 1990; R. L. Sabina, N. Ogasawara, and E. W. Holmes, Mol. Cell. Biol. 9:2244-2246, 1989). By using transfection studies with native, mutant, and chimeric minigene constructs, two steps in RNA processing that determine the ratio of these two transcripts have been identified. The first step is recognition of this exon in the primary transcript. The primary transcript is subject to alternative splicing in which exon 2 is either recognized and thereby included in the mature mRNA or is ignored and retained in a composite intron containing intron 1-exon 2-intron 2. The following properties of the primary transcript influence exon recognition. (i) Exon 2 is intrinsically difficult to recognize, possibly because of its small size (only 12 bases) and/or a suboptimal 5' donor site at the exon 2-intron 2 boundary. (ii) Intron 2 plays a permissive role in recognition of exon 2 because it is removed at a relatively slow rate, presumably because of the suboptimal polypyrimidine tract in the putative 3' branch site. The second step in RNA processing that influences the ratio of mature transcripts produced from the AMPD1 gene occurs subsequent to the ligation of exon 2 to exon 1. An RNA intermediate, composed of exon 1-exon 2-intron 2-exon 3, is produced in the first processing step, but it is variably retained in the nucleus. Retention of this intermediate in the nucleus is associated with accumulation of the mature mRNA containing exon 2, while cytoplasmic escape of this intermediate is reactions, exon recognition and nucleocytoplasmic partitioning, determine the relative abundance of alternative mRNAs derived from the AMPD1 gene. Images PMID:1922051

  6. The dynamics of the alternatively spliced NOL7 gene products and role in nucleolar architecture

    PubMed Central

    Kinor, Noa

    2011-01-01

    Three alternatively spliced forms of the human NOL7 gene coding for relatively small proteins were identified. The two shorter forms were generated by intron retention events, and each isoform was differently localized within the cell. The NOL7-SP1 long form (29 kD) localized to the nucleolus, SP2 was nucleoplasmic, while SP3 was distributed throughout the whole cell. NOL7-SP1 was confined to the nucleolar granular component, and during cell division disassociated from the nucleolus. Knockdown of NOL7-SP1 levels abrogated nucleolar architecture, in particular the internal regions, and reduced cell proliferation. Analysis of the nucleolar dynamics of the SP1 protein during interphase showed nucleolar high binding affinity. Dissection of protein domains showed that nucleolar targeting was mediated by a unique C-terminal nucleolar localization sequence (NoLS). However, this sequence was not sufficient for conferring high binding affinity, which required additional regions of the protein. Our analysis shows that NOL7 is important for maintaining internal nucleolar structure and cell growth rates, and that while specific protein localization can be obtained by specific short localization motifs, nucleolar residency through binding must be mediated by a synergistic combination of protein modules. PMID:21818416

  7. The relation between first-graders' reading level and vowel production variability in real and nonsense words: A temporal analysis

    NASA Astrophysics Data System (ADS)

    Lydtin, Kimberly; Fowler, Anne; Bell-Berti, Fredericka

    2002-05-01

    The focus of this study is to determine if children who are poor readers produce vowels with greater variability than children with normal reading ability, since earlier research has indicated possible links between phonological difficulty, speech production variation, and reading problems. In continuation of our past research [K. Lydtin, A. Fowler, and F. Bell-Berti, J. Acoust. Soc. Am. 110, 2704 (2001)], where we looked at the spectral aspects of vowel production, we will report the results of our study of vowel duration and its variability in poor and good readers. The vowels chosen for this study are /smcapi/, /ɛ/, and /æ/ in real and nonsense words occurring in both blocked and random presentation. [Work supported by U.S. Dept. of Education, McNair Scholars Program.

  8. Contribution of Near Real Time MODIS-Based Forest Disturbance Detection Products to a National Forest Threat Early Warning System

    NASA Technical Reports Server (NTRS)

    Spruce, Joseph; Hargrove, William; Gasser, Gerald; Smoot, James; Kuper, Philip

    2011-01-01

    U.S. forests occupy approx. 751 million acres (approx. 1/3 of total land). These forests are exposed to multiple biotic and abiotic threats that collectively damage extensive acreages each year. Hazardous forest disturbances can threaten human life and property, bio-diversity and water supplies. Timely regional forest monitoring products are needed to aid forest management and decision making by the US Forest Service and its state and private partners. Daily MODIS data products provide a means to monitor regional forest disturbances on a weekly basis. In response, we began work in 2006 to develop a Near Real Time (NRT) forest monitoring capability, based on MODIS NDVI data, as part of a national forest threat early warning system (EWS)

  9. [Simple and rapid screening for psychotropic natural products using Direct Analysis in Real Time (DART)-TOFMS].

    PubMed

    Kawamura, Maiko; Kikura-Hanajiri, Ruri; Goda, Yukihiro

    2009-06-01

    Direct Analysis in Real Time (DART) is a novel ionization technique that provides for the rapid ionization of small molecules under ambient conditions. To investigate the trend of non-controlled psychotropic plants of abuse in Japan, a rapid screening method, without sample preparation, was developed using DART-time of flight mass spectrometer (TOFMS) for plant products. The major psychotropic constituents of these products were determined using liquid chromatography-mass spectrometry (LC/MS). As a result of the DART-TOFMS analyses of 36 products, the protonated molecular ions [M+H](+), corresponding to 6 kinds of major hallucinogenic constituents (mescaline, salvinorin A, N,N-dimethyltryptamine, harmine, harmaline and lysergamide), were detected in 21 products. It was possible to estimate their accurate elemental compositions through exact mass measurements. These results were consistent with those of the LC/MS analyses and the contents of the 6 psychotropic constituents were in the range from 0.05 to 45 microg/mg. Typical controlled narcotic drugs, tetrahydrocannabinol, opioid alkaloids and psilocin were also directly detected in marijuana cigarette, opium gum and magic mushroom respectively. Although it is difficult to estimate the matrix effects caused by other plant ingredients, the DART-TOFMS could be useful as a simple and rapid screening method for the targeted psychotropic natural products, because it provides the molecular information of the target compounds without time-consuming extraction and pre-treatment steps. PMID:19483414

  10. Crystal structure of A. aeolicus LpxC with bound product suggests alternate deacetylation mechanism.

    PubMed

    Miller, Matthew D; Gao, Ning; Ross, Philip L; Olivier, Nelson B

    2015-09-01

    UDP-3-O-acyl-N-acetylglucosamine deacetylase (LpxC) is the first committed step to form lipid A, an essential component of the outer membrane of Gram-negative bacteria. As it is essential for the survival of many pathogens, LpxC is an attractive target for antibacterial therapeutics. Herein, we report the product-bound co-crystal structure of LpxC from the acheal Aquifex aeolicus solved to 1.6 Å resolution. We identified interactions by hydroxyl and hydroxymethyl substituents of the product glucosamine ring that may enable new insights to exploit waters in the active site for structure-based design of LpxC inhibitors with novel scaffolds. By using this product structure, we have performed quantum mechanical modeling on the substrate in the active site. Based on our results and published experimental data, we propose a new mechanism that may lead to a better understanding of LpxC catalysis and inhibition. PMID:26177919

  11. An analysis of alternative technologies for the removal of ethylene from the CELSS biomass production chamber

    NASA Technical Reports Server (NTRS)

    Rakow, Allen L.

    1995-01-01

    A variety of technologies were analyzed for their potential to remove ethylene from the CELSS Biomass Production Chamber (BPC). During crop production (e.g., lettuce, wheat, soybean, potato) in the BPC ethylene can accumulate in the airspace and subsequently affect plant viability. The chief source of ethylene is the plants themselves which reside in plastic trays containing nutrient solution. The main sink for ethylene is chamber leakage. The removal technology can be employed when deleterious levels (e.g., 50 ppb for potato) of ethylene are exceeded in the BPC and perhaps to optimize the plant growth process once a better understanding is developed of the relationship between exogenous ethylene concentration and plant growth. The technologies examined were catalytic oxidation, molecular sieve, cryotrapping, permanganate absorption, and UV degradation. Upon analysis, permanganate was chosen as the most suitable method. Experimental data for ethylene removal by permanganate during potato production was analyzed in order to design a system for installation in the BPC air duct. In addition, an analysis of the impact on ethylene concentration in the BPC of integrating the Breadboard Scale Aerobic Bioreactor (BSAB) with the BPC was performed. The result indicates that this unit has no significant effect on the ethylene material balance as a source or sink.

  12. Real and virtual N N ¯ pair production near the threshold

    NASA Astrophysics Data System (ADS)

    Dmitriev, V. F.; Milstein, A. I.; Salnikov, S. G.

    2016-02-01

    Nucleon-antinucleon optical potential, which explains the experimental data for the processes e+e-→p p ¯ and e+e-→pions near the threshold of p p ¯ pair production, is suggested. To obtain this potential, we have used the available experimental data for p p ¯ scattering, p p ¯ pair production in e+e- annihilation, and the ratio of electromagnetic form factors of a proton in the timelike region. It turns out that final-state interaction via the optical potential allows one to reproduce the available experimental data with good accuracy. Our results for the cross sections of the e+e-→6 π process near the threshold of p p ¯ pair production are in agreement with the recent experiments.

  13. Improving AirNow Air Quality Products with NASA Near-Real-Time Remote Sensing Data (Invited)

    NASA Astrophysics Data System (ADS)

    Dye, T.; Pasch, A. N.; DeWinter, J. L.; Haderman, M.; Szykman, J.; White, J. E.; van Donkelaar, A.; Martin, R.

    2013-12-01

    The U.S. Environmental Protection Agency's (EPA) AirNow program provides the public with real-time and forecasted air quality conditions. Millions of people each day use it to protect their health. The AirNow program (http://www.airnow.gov), reports ground-level ozone (O3) and fine particulate matter (PM2.5) in a standardized index called the Air Quality Index (AQI). AirNow aggregates information from over 130 state, local, and federal air quality agencies and provides tools for over 2,000 agency staff responsible for monitoring, forecasting, and communicating local air quality. Each hour, AirNow systems generate thousands of maps and products. This presentation will describe how AirNow is benefiting from NASA's remote sensing data. We will describe two applications of NASA near-real-time remote sensing data within AirNow through case studies, focusing specifically on days when large spatial gradients in AQI and wildfire smoke impacts were observed. The first case study will show how AirNow is merging satellite-estimated PM2.5 concentrations into the AQI maps via the AirNow Satellite Data Processor (ASDP). AirNow derives these satellite estimates using NASA/NOAA satellite aerosol optical depth (AOD) retrievals and GEOS-Chem modeled ratios of surface PM2.5 concentrations to AOD. The second case study will show how NASA's Global Image Browse Services (GIBS) provides a near-real-time satellite product in AirNow-Tech for agency users to quickly identify smoke plumes and access air quality conditions in data-sparse areas during wildland fires.

  14. OceanNOMADS: Real-time and retrospective access to operational U.S. ocean prediction products

    NASA Astrophysics Data System (ADS)

    Harding, J. M.; Cross, S. L.; Bub, F.; Ji, M.

    2011-12-01

    The National Oceanic and Atmospheric Administration (NOAA) National Operational Model Archive and Distribution System (NOMADS) provides both real-time and archived atmospheric model output from servers at the National Centers for Environmental Prediction (NCEP) and National Climatic Data Center (NCDC) respectively (http://nomads.ncep.noaa.gov/txt_descriptions/marRutledge-1.pdf). The NOAA National Ocean Data Center (NODC) with NCEP is developing a complementary capability called OceanNOMADS for operational ocean prediction models. An NCEP ftp server currently provides real-time ocean forecast output (http://www.opc.ncep.noaa.gov/newNCOM/NCOM_currents.shtml) with retrospective access through NODC. A joint effort between the Northern Gulf Institute (NGI; a NOAA Cooperative Institute) and the NOAA National Coastal Data Development Center (NCDDC; a division of NODC) created the developmental version of the retrospective OceanNOMADS capability (http://www.northerngulfinstitute.org/edac/ocean_nomads.php) under the NGI Ecosystem Data Assembly Center (EDAC) project (http://www.northerngulfinstitute.org/edac/). Complementary funding support for the developmental OceanNOMADS from U.S. Integrated Ocean Observing System (IOOS) through the Southeastern University Research Association (SURA) Model Testbed (http://testbed.sura.org/) this past year provided NODC the analogue that facilitated the creation of an NCDDC production version of OceanNOMADS (http://www.ncddc.noaa.gov/ocean-nomads/). Access tool development and storage of initial archival data sets occur on the NGI/NCDDC developmental servers with transition to NODC/NCCDC production servers as the model archives mature and operational space and distribution capability grow. Navy operational global ocean forecast subsets for U.S waters comprise the initial ocean prediction fields resident on the NCDDC production server. The NGI/NCDDC developmental server currently includes the Naval Research Laboratory Inter-America Seas

  15. Reformers for the production of hydrogen from methanol and alternative fuels for fuel cell powered vehicles

    SciTech Connect

    Kumar, R.; Ahmed, S.; Krumpelt, M.; Myles, K.M.

    1992-08-01

    The objective of this study was (i) to assess the present state of technology of reformers that convert methanol (or other alternative fuels) to a hydrogen-rich gas mixture for use in a fuel cell, and (ii) to identify the R&D needs for developing reformers for transportation applications. Steam reforming and partial oxidation are the two basic types of fuel reforming processes. The former is endothermic while the latter is exothermic. Reformers are therefore typically designed as heat exchange systems, and the variety of designs used includes shell-and-tube, packed bed, annular, plate, and cyclic bed types. Catalysts used include noble metals and oxides of Cu, Zn, Cr, Al, Ni, and La. For transportation applications a reformer must be compact, lightweight, and rugged. It must also be capable of rapid start-up and good dynamic performance responsive to fluctuating loads. A partial oxidation reformer is likely to be better than a steam reformer based on these considerations, although its fuel conversion efficiency is expected to be lower than that of a steam reformer. A steam reformer better lends itself to thermal integration with the fuel cell system; however, the thermal independence of the reformer from the fuel cell stack is likely to yield much better dynamic performance of the reformer and the fuel cell propulsion power system. For both steam reforming and partial oxidation reforming, research is needed to develop compact, fast start-up, and dynamically responsive reformers. For transportation applications, steam reformers are likely to prove best for fuel cell/battery hybrid power systems, and partial oxidation reformers are likely to be the choice for stand-alone fuel cell power systems.

  16. Reformers for the production of hydrogen from methanol and alternative fuels for fuel cell powered vehicles

    SciTech Connect

    Kumar, R.; Ahmed, S.; Krumpelt, M.; Myles, K.M.

    1992-08-01

    The objective of this study was (i) to assess the present state of technology of reformers that convert methanol (or other alternative fuels) to a hydrogen-rich gas mixture for use in a fuel cell, and (ii) to identify the R D needs for developing reformers for transportation applications. Steam reforming and partial oxidation are the two basic types of fuel reforming processes. The former is endothermic while the latter is exothermic. Reformers are therefore typically designed as heat exchange systems, and the variety of designs used includes shell-and-tube, packed bed, annular, plate, and cyclic bed types. Catalysts used include noble metals and oxides of Cu, Zn, Cr, Al, Ni, and La. For transportation applications a reformer must be compact, lightweight, and rugged. It must also be capable of rapid start-up and good dynamic performance responsive to fluctuating loads. A partial oxidation reformer is likely to be better than a steam reformer based on these considerations, although its fuel conversion efficiency is expected to be lower than that of a steam reformer. A steam reformer better lends itself to thermal integration with the fuel cell system; however, the thermal independence of the reformer from the fuel cell stack is likely to yield much better dynamic performance of the reformer and the fuel cell propulsion power system. For both steam reforming and partial oxidation reforming, research is needed to develop compact, fast start-up, and dynamically responsive reformers. For transportation applications, steam reformers are likely to prove best for fuel cell/battery hybrid power systems, and partial oxidation reformers are likely to be the choice for stand-alone fuel cell power systems.

  17. Impact of a Real Microgravity on the Productivity of Tomato Plants and Resistance to Viruses

    NASA Astrophysics Data System (ADS)

    Mishchenko, L. T.; Dunich, A. A.; Danilova, O. I.

    2013-02-01

    It was detected that space flight factors increased tomato productivity that is important for using such plants as cosmonauts nutrition in long-term space missions. It was revealed that tomato plants, whose seeds were exposed to long space flight conditions, are more resistant to viruses and have higher polyphenols concentration than in the stationary control.

  18. Grammatical Gender in L2: A Production or a Real-Time Processing Problem?

    ERIC Educational Resources Information Center

    Gruter, Theres; Lew-Williams, Casey; Fernald, Anne

    2012-01-01

    Mastery of grammatical gender is difficult to achieve in a second language (L2). This study investigates whether persistent difficulty with grammatical gender often observed in the speech of otherwise highly proficient L2 learners is best characterized as a production-specific performance problem, or as difficulty with the retrieval of gender…

  19. HIGH SPEED REAL TIME REMOVAL OF UNDESIRABLE PRODUCT FROM FOOD PROCESSING LINES

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Removal of undesirable defects and contaminants in food products is a concern for both food safety and food quality reasons. The presence of such materials is therefore a major component of quality grading standards. It can also have an effect on the accessibility of foreign markets and a producer...

  20. Alternative approaches to the manufacture of plant protein products from grain legumes.

    PubMed

    Braudo, E E; Danilenko, A N; Dianova, V T; Krokha, N G

    2001-10-01

    A biotechnological method for the modification of grain legume meals (pea, faba bean) is proposed. The essence of this method is the inducing of biochemical processes, which occur at the germination, through the treatment of a meal with an acid protease. Modified meals can replace soybean protein isolate as meat extenders. In particular, the modification of pea meal improves the colour of combined meat products and eliminates their beany off-flavour. The concentration of six-carbon aldehydes in combined sausages is reduced after the modification of pea meal. PMID:11712243

  1. Biosolids management strategies: an evaluation of energy production as an alternative to land application.

    PubMed

    Egan, Maureen

    2013-07-01

    Currently, more than half of the biosolids produced within the USA are land applied. Land application of biosolids introduces organic contaminants into the environment. There are potential ecological and human health risks associated with land application of biosolids. Biosolids may be used as a renewable energy source. Nutrients may be recovered from biosolids used for energy generation for use as fertilizer. The by-products of biosolids energy generation may be used beneficially in construction materials. It is recommended that energy generation replace land application as the leading biosolids management strategy. PMID:23529399

  2. Two-photon dilepton production in proton-proton collisions: Two alternative approaches

    NASA Astrophysics Data System (ADS)

    Łuszczak, Marta; Schäfer, Wolfgang; Szczurek, Antoni

    2016-04-01

    We investigate different methods to incorporate the effect of photons in hard processes. We compare the two different approaches used for calculating cross sections for the two-photon p p →l+l-X process. In one of the approaches the photon is treated as a collinear parton in the proton. In the second approach the recently proposed kT factorization method is used. We discuss how results of the collinear parton model depend on the initial condition for the QCD evolution and discuss an approximate treatment where the photon is excluded from the combined QCD-QED evolution. We demonstrate that it is not necessary to put the photon into the evolution equation as is often done, but it is sufficient to use a simplified approach in which the photon couples to quarks and antiquarks, which by themselves undergo DGLAP evolution equations. We discuss the sensitivity of the results to the choice of structure function parametrization and experimental cuts in the kT factorization approach. We explicitly display regions of x and Q2 (arguments of structure functions) relevant for different experiments. We compare the results of our calculations with recent experimental data for dilepton production and find that in most cases the contribution of the photon-photon mechanism is rather small. We discuss how to enhance the photon-photon contribution. We also compare our results to those of recent measurements of exclusive and semiexclusive e+e- pair production with certain experimental data by the CMS Collaboration.

  3. Effects of Interannual Climate Variability on Water Availability and Productivity in Capoeira and Crops Under Traditional and Alternative Shifting Cultivation

    NASA Technical Reports Server (NTRS)

    Guild, Liane S.; Sa, Tatiana D. A.; Carvalho, Claudio J. R.; Potter, Christopher S.; Wickel, Albert J.; Brienza, Silvio, Jr.; Kato, Maria doSocorro A.; Kato, Osvaldo; Brass, James (Technical Monitor)

    2002-01-01

    Regenerating forests play an important role in long-term carbon sequestration and sustainable landuse as they act as potentially important carbon and nutrient sinks during the shifting agriculture fallow period. The long-term functioning of capoeira. is increasingly threatened by a shortening fallow period during shifting cultivation due to demographic pressures and associated increased vulnerability to severe climatic events. Declining productivity and functioning of fallow forests of shifting cultivation combined with progressive loss of nutrients by successive burning and cropping activities has resulted in declining agricultural productivity. In addition to the effects of intense land use practices, droughts associated with El Nino events are becoming more frequent and severe in moist tropical forests and negative effects on capoeira productivity could be considerable. In Igarape-Acu (near Belem, Para), we hypothesize that experimental alternative landuse/clearing practices (mulching and fallow vegetation improvement by planting with fast-growing leguminous tree species) may make capoeira and agriculture more resilient to the effects of agricultural pressures and drought through (1) increased biomass, soil organic matter and associated increase in soil water storage, and nutrient retention and (2) greater rooting depth of trees planted for fallow improvement. This experimental practice (moto mechanized chop-and-mulch with fallow improvement) has resulted increased soil moisture during the cropping phase, reduced loss of nutrients and organic matter, and higher rates of secondary-forest biomass accumulation. We present preliminary data on water relations during the dry season of 2001 in capoeira and crops for both traditional slash-and-burn and alternative chop-and-mulch practices. These data will be used to test IKONOS data for the detection of moisture status differences. The principal goal of the research is to determine the extent to which capoeira and

  4. Stakeholders' perspectives on the regulation and integration of complementary and alternative medicine products in Lebanon: a qualitative study

    PubMed Central

    2011-01-01

    Background The regulation of the markets for Complementary and Alternative Medicine (CAM) products presents a global challenge. There is a dearth of studies that have examined or evaluated the regulatory policies of CAM products in the Eastern Mediterranean Region (EMR). We investigate the regulatory frameworks and the barriers for the proper regulation and integration of CAM products in Lebanon, as an example of an EMR country with a weak public infrastructure. Methods We utilized a qualitative study design involving a series of semi-structured interviews with stakeholders of the CAM market in Lebanon. Snowball sampling was used to identify interviewees; interviews continued until the "saturation" point was reached. A total of 16 interviews were carried out with decision makers, representatives of professional associations, academic researchers, CAM product importers, policy makers and a media representative. Interviews were transcribed and thematic analysis of scripts was carried out. Results There was a consensus among all stakeholders that the regulation of the market for CAM products in Lebanon needs to be strengthened. Thematic analysis identified a number of impediments jeopardizing the safety of public consumption and hindering the integration of CAM therapies into mainstream medicine; including: weak infrastructure, poor regulation, ineffective policies and politics, weak CAM awareness and sub-optimal coordination and cooperation among stakeholders. With respect to policy instruments, voluntary instruments (self regulation) were deemed ineffective by stakeholders due to poor awareness of both users and providers on safe use of CAM products. Stakeholders' rather recommended the adoption of a combination of mixed (enhancing public awareness and integration of CAM into medical and nursing curricula) and compulsory (stricter governmental regulation) policy instruments for the regulation of the market for CAM products. Conclusions The current status quo with

  5. Electrical Structure of Real Thunderstorms in Relation to Energetic Radiation Production

    NASA Astrophysics Data System (ADS)

    Stolzenburg, M.; Marshall, T. C.

    2015-12-01

    Recent observations have shown that highly energetic radiation in various forms is produced in association with thunderstorms. Many of the developing models of energetic radiation production rely on characteristics of the underlying thunderstorm electrical structure that are more or less known. This presentation will give an overview of electric field and net charge structures observed inside thunderstorms as they may relate to the production of energetic radiation. Locations within clouds of lightning initiation and altitudes of the main charges involved in intracloud and cloud-to-ground lightning flashes will be covered. Typical maximum electric field magnitudes and their altitudes within storms will be discussed, along with the few cases of extreme field values observed near lightning initiations. The variability in the large scale charge structure and the possible importance of smaller scale features as a storm evolves through its lifecycle will also be examined.

  6. Paralinguistic mechanisms of production in human “beatboxing”: A real-time magnetic resonance imaging study

    PubMed Central

    Proctor, Michael; Bresch, Erik; Byrd, Dani; Nayak, Krishna; Narayanan, Shrikanth

    2013-01-01

    Real-time Magnetic Resonance Imaging (rtMRI) was used to examine mechanisms of sound production by an American male beatbox artist. rtMRI was found to be a useful modality with which to study this form of sound production, providing a global dynamic view of the midsagittal vocal tract at frame rates sufficient to observe the movement and coordination of critical articulators. The subject's repertoire included percussion elements generated using a wide range of articulatory and airstream mechanisms. Many of the same mechanisms observed in human speech production were exploited for musical effect, including patterns of articulation that do not occur in the phonologies of the artist's native languages: ejectives and clicks. The data offer insights into the paralinguistic use of phonetic primitives and the ways in which they are coordinated in this style of musical performance. A unified formalism for describing both musical and phonetic dimensions of human vocal percussion performance is proposed. Audio and video data illustrating production and orchestration of beatboxing sound effects are provided in a companion annotated corpus. PMID:23363120

  7. Grammatical Planning Units During Real-Time Sentence Production in Speakers With Agrammatic Aphasia and Healthy Speakers

    PubMed Central

    Yoshida, Masaya; Thompson, Cynthia K.

    2015-01-01

    Purpose Grammatical encoding (GE) is impaired in agrammatic aphasia; however, the nature of such deficits remains unclear. We examined grammatical planning units during real-time sentence production in speakers with agrammatic aphasia and control speakers, testing two competing models of GE. We queried whether speakers with agrammatic aphasia produce sentences word by word without advanced planning or whether hierarchical syntactic structure (i.e., verb argument structure; VAS) is encoded as part of the advanced planning unit. Method Experiment 1 examined production of sentences with a predefined structure (i.e., “The A and the B are above the C”) using eye tracking. Experiment 2 tested production of transitive and unaccusative sentences without a predefined sentence structure in a verb-priming study. Results In Experiment 1, both speakers with agrammatic aphasia and young and age-matched control speakers used word-by-word strategies, selecting the first lemma (noun A) only prior to speech onset. However, in Experiment 2, unlike controls, speakers with agrammatic aphasia preplanned transitive and unaccusative sentences, encoding VAS before speech onset. Conclusions Speakers with agrammatic aphasia show incremental, word-by-word production for structurally simple sentences, requiring retrieval of multiple noun lemmas. However, when sentences involve functional (thematic to grammatical) structure building, advanced planning strategies (i.e., VAS encoding) are used. This early use of hierarchical syntactic information may provide a scaffold for impaired GE in agrammatism. PMID:25908309

  8. Evaluation of an alternative extraction procedure for enterotoxin determination in dairy products.

    PubMed

    Meyrand, A; Atrache, V; Bavai, C; Montet, M P; Vernozy-Rozand, C

    1999-06-01

    A concentration protocol based on trichloroacetic acid precipitation was evaluated and compared with the reference method using dialysis concentration. Different quantities of purified staphylococcal enterotoxins were added to pasteurized Camembert-type cheeses. Detection of enterotoxins in these cheeses was performed using an automated detection system. Raw goat milk Camembert-type cheeses involved in a staphylococcal food poisoning were also tested. Both enterotoxin extraction methods allowed detection of the lowest enterotoxin concentration level used in this study (0.5 ng g-1). Compared with the dialysis concentration method, TCA precipitation of staphylococcal enterotoxins was 'user-friendly' and less time-consuming. These results suggest that TCA precipitation is a rapid (1 h), simple and reliable method of extracting enterotoxin from food which gives excellent recovery from dairy products. PMID:10389254

  9. Rice gluten meal as an alternative by-product feed for growing dairy calves.

    PubMed

    Kumar, Rohit; Thakur, Sudarshan Singh; Mahesh, M S

    2016-03-01

    This experiment aimed at studying the nutritional characteristics and feeding value of rice gluten meal (RGM, a wet-milling by-product of rice) in growing dairy calves. RGM contained 464 g/kg of crude protein with 821 and 196 g/kg nitrogen (N) of borate-phosphate insoluble N and acid detergent insoluble N, respectively, which were higher (P < 0.05) than groundnut cake (GNC). In vitro gas production, organic matter digestibility and energy values were comparable between RGM and GNC. For in vivo trial, 18 Karan-Fries calves (6-12 months) were randomly assigned into three groups based on comparable body weight and age. The first group (GP-I) was fed concentrate mixture containing mainly GNC as protein source, whilst it was replaced by RGM up to 50 and 75 % on N basis, in second (GP-II) and third (GP-III) groups, respectively. Thus, RGM constituted 140 and 210 g/kg of concentrate mixture of GP-II and GP-III, respectively. In addition, all animals were offered chopped green maize and wheat straw for the whole experimental period of 90 days. Results revealed that there was no difference in intake and digestibility of nutrients, N balance, average daily gain (ADG) and feed efficiency among three groups. Nevertheless, RGM-based diets produced cost-effective ADG than GP-I. Furthermore, experimental calves did not differ in haematological variables like glucose, blood urea N, plasma proteins and non-esterified fatty acids. This study demonstrated that RGM could be incorporated successfully in the concentrate mixture, replacing 75 % of GNC without any discernable compromise in the performance of growing calves. PMID:26885987

  10. Use of hazard analysis critical control point and alternative treatments in the production of apple cider.

    PubMed

    Senkel, I A; Henderson, R A; Jolbitado, B; Meng, J

    1999-07-01

    The purpose of this study was to evaluate the practices of Maryland cider producers and determine whether implementing hazard analysis critical control point (HACCP) would reduce the microbial contamination of cider. Cider producers (n = 11) were surveyed to determine existing manufacturing practices and sanitation. A training program was then conducted to inform operators of safety issues, including contamination with Escherichia coli O157:H7, and teach HACCP concepts and principles, sanitation procedures, and good manufacturing practice (GMP). Although all operators used a control strategy from one of the model HACCP plans provided, only one developed a written HACCP plan. None developed specific GMP, sanitation standard operating procedures, or sanitation monitoring records. Six operators changed or added production controls, including the exclusion of windfall apples, sanitizing apples chemically and by hot dip, and cider treatment with UV light or pasteurization. Facility inspections indicated improved sanitation and hazard control but identified ongoing problems. Microbiological evaluation of bottled cider before and after training, in-line apples, pomace, cider, and inoculated apples was conducted. E. coli O157:H7, Salmonella, or Staphylococcus aureus were not found in samples of in-line apple, pomace, and cider, or bottled cider. Generic E. coli was not isolated on in-coming apples but was found in 4 of 32 (13%) in-line samples and 3 of 17 (18%) bottled fresh cider samples, suggesting that E. coli was introduced during in-plant processing. To produce pathogen-free cider, operators must strictly conform to GMP and sanitation procedures in addition to HACCP controls. Controls aimed at preventing or eliminating pathogens on source apples are critical but alone may not be sufficient for product safety. PMID:10419272

  11. A Recommender System for an IPTV Service Provider: a Real Large-Scale Production Environment

    NASA Astrophysics Data System (ADS)

    Bambini, Riccardo; Cremonesi, Paolo; Turrin, Roberto

    In this chapter we describe the integration of a recommender system into the production environment of Fastweb, one of the largest European IP Television (IPTV) providers. The recommender system implements both collaborative and content-based techniques, suitable tailored to the specific requirements of an IPTV architecture, such as the limited screen definition, the reduced navigation capabilities, and the strict time constraints. The algorithms are extensively analyzed by means of off-line and on-line tests, showing the effectiveness of the recommender systems: up to 30% of the recommendations are followed by a purchase, with an estimated lift factor (increase in sales) of 15%.

  12. Biogas production in Kentucky: A best management practice alternative for nonpoint source pollution prevention

    SciTech Connect

    Zourarakis, D.P.; Coleman, S.A.; Thom, W.O.

    1996-12-31

    Despite continued conservation efforts on the part of private landowners, citizens groups, universities, and government agencies, the lack of adequate animal waste management systems still poses a significant threat to both water and land quality in Kentucky. Recent surveys indicate that only a fraction of the animal confinement units in the state have waste management systems in good operating condition. Biogas production systems are not presently included as a technological option or {open_quotes}best management practice{close_quotes} (BMP) for recycling animal wastes and are not eligible for Cost Share financial aid programs. Abundant animal manure is produced as a reasonably collectible resource in farm operations where dairy cattle, swine, and poultry are raised. Broiler and layer houses are rapidly proliferating in the western part of the state. This paper assesses the economic viability of using a low-cost, floating cover lagoon technology to collect biogas and generate electricity in several types of animal raising operations. In cases where the biogas energy can be used effectively on the farm and the technology receives partial funding as a BMP, the technology is economically viable.

  13. The potentiality of botanicals and their products as an alternative to chemical insecticides to sandflies (Diptera: Psychodidae): a review.

    PubMed

    Dinesh, Diwakar Singh; Kumari, Seema; Kumar, Vijay; Das, Pradeep

    2014-03-01

    Use of chemical pesticides is the current method for controlling sandflies. However, resistance is being developed in sandflies against the insecticide of choice that is DDT (dichlorodiphenyl trichloroethane). Botanicals have potential to act as an alternative to chemical insecticides as the crude extracts and active molecules of some plants show insecticidal effect to sandflies. This will lead to safe, easy and environment friendly method for control of sandflies. Therefore, information regarding botanicals acting as alternative to chemical insecticide against sandflies assumes importance in the context of development of resistance to insecticides as well as to prevent environment from contamination. This review deals with some plants and their products having repellent and insecticidal effect to sandflies in India and abroad. Different methods of extraction and their bioassay on sandflies have been emphasized in the text. Various extracts of some plants like Ricinus communis (Euphorbiaceae), Solanum jasminoides (Solanaceae), Bougainvillea glabra (Nyctaginaceae), Capparis spinosa (Capparidaceae), Acalypha fruticosa (Euphorbiaceae) and Tagetes minuta (Asteraceae) had shown repellent/insecticidal effect on sandflies. This review will be useful in conducting the research work to find out botanicals of Indian context having insecticidal effect on sandflies. PMID:24717195

  14. Electromagnetic Productions of the Hyperon and the Hypertriton Using Real and Virtual Photons

    NASA Astrophysics Data System (ADS)

    Mart, T.

    2010-04-01

    The electromagnetic production of kaon-hyperon on the nucleon has been studied by means of a diagrammatic technique. All important resonances listed by the Particle Data Group up to spin 5/2 are included in this analysis. To extract the unknown coupling constants the new data from CLAS, SAPHIR, LEPS, and GRAAL collaborations have been used. In this paper we report on the preliminary results of this analysis. The available elementary model along with the modern nuclear wave functions have been used to analyze the process of photoas well as electroproduction of the hypertriton off the 3He. In this analysis we found that a proper treatment of Fermi motion is essential for describing the process, whereas the few available experimental data favor the assumption that the initial nucleon is off-shell and the final hyperon is on-shell. The influence of the higher partial waves in this process is also investigated. We also discuss the prospect of investigating kaon production off 4He.

  15. Redirecting photosynthetic electron flow into light-driven synthesis of alternative products including high-value bioactive natural compounds.

    PubMed

    Lassen, Lærke Münter; Nielsen, Agnieszka Zygadlo; Ziersen, Bibi; Gnanasekaran, Thiyagarajan; Møller, Birger Lindberg; Jensen, Poul Erik

    2014-01-17

    Photosynthesis in plants, green algae, and cyanobacteria converts solar energy into chemical energy in the form of ATP and NADPH, both of which are used in primary metabolism. However, often more reducing power is generated by the photosystems than what is needed for primary metabolism. In this review, we discuss the development in the research field, focusing on how the photosystems can be used as synthetic biology building blocks to channel excess reducing power into light-driven production of alternative products. Plants synthesize a large number of high-value bioactive natural compounds. Some of the key enzymes catalyzing their biosynthesis are the cytochrome P450s situated in the endoplasmic reticulum. However, bioactive compounds are often synthesized in low quantities in the plants and are difficult to produce by chemical synthesis due to their often complex structures. Through a synthetic biology approach, enzymes with a requirement for reducing equivalents as cofactors, such as the cytochrome P450s, can be coupled directly to the photosynthetic energy output to obtain environmentally friendly production of complex chemical compounds. By relocating cytochrome P450s to the chloroplasts, reducing power can be diverted toward the reactions catalyzed by the cytochrome P450s. This provides a sustainable production method for high-value compounds that potentially can solve the problem of NADPH regeneration, which currently limits the biotechnological uses of cytochrome P450s. We describe the approaches that have been taken to couple enzymes to photosynthesis in vivo and to photosystem I in vitro and the challenges associated with this approach to develop new green production platforms. PMID:24328185

  16. An assessment of potential degradation products in the gas-phase reactions of alternative fluorocarbons in the troposphere

    NASA Technical Reports Server (NTRS)

    Niki, Hiromi

    1990-01-01

    Tropospheric chemical transformations of alternative hydrofluorocarbons (HCF's) and hydrochlorofluorocarbons (HCFC's) are governed by hydroxyl radical initiated oxidation processes, which are likely to be analogous to those known for alkanes and chloroalkanes. A schematic diagram is used to illustrate plausible reaction mechanisms for their atmospheric degradation, where R, R', and R'' denote the F- and/or Cl-substituted alkyl groups derived from HCF's and HCFC's subsequent th the initial H atom abstraction by HO radicals. At present, virtually no kinetic data exist for the majority of these reactions, particularly for those involving RO. Potential degradation intermediates and final products include a large variety of fluorine- and/or chlorine-containing carbonyls, acids, peroxy acids, alcohols, hydrogen peroxides, nitrates and peroxy nitrates, as summarized in the attached table. Probably atmospheric lifetimes of these compounds were also estimated. For some carbonyl and nitrate products shown in this table, there seem to be no significant gas-phase removal mechanisms. Further chemical kinetics and photochemical data are needed to quantitatively assess the atmospheric fate of HCF's and HCFC's, and of the degradation products postulated in this report.

  17. A wavelet-based non-linear autoregressive with exogenous inputs (WNARX) dynamic neural network model for real-time flood forecasting using satellite-based rainfall products

    NASA Astrophysics Data System (ADS)

    Nanda, Trushnamayee; Sahoo, Bhabagrahi; Beria, Harsh; Chatterjee, Chandranath

    2016-08-01

    Although flood forecasting and warning system is a very important non-structural measure in flood-prone river basins, poor raingauge network as well as unavailability of rainfall data in real-time could hinder its accuracy at different lead times. Conversely, since the real-time satellite-based rainfall products are now becoming available for the data-scarce regions, their integration with the data-driven models could be effectively used for real-time flood forecasting. To address these issues in operational streamflow forecasting, a new data-driven model, namely, the wavelet-based non-linear autoregressive with exogenous inputs (WNARX) is proposed and evaluated in comparison with four other data-driven models, viz., the linear autoregressive moving average with exogenous inputs (ARMAX), static artificial neural network (ANN), wavelet-based ANN (WANN), and dynamic nonlinear autoregressive with exogenous inputs (NARX) models. First, the quality of input rainfall products of Tropical Rainfall Measuring Mission Multi-satellite Precipitation Analysis (TMPA), viz., TRMM and TRMM-real-time (RT) rainfall products is assessed through statistical evaluation. The results reveal that the satellite rainfall products moderately correlate with the observed rainfall, with the gauge-adjusted TRMM product outperforming the real-time TRMM-RT product. The TRMM rainfall product better captures the ground observations up to 95 percentile range (30.11 mm/day), although the hit rate decreases for high rainfall intensity. The effect of antecedent rainfall (AR) and climate forecast system reanalysis (CFSR) temperature product on the catchment response is tested in all the developed models. The results reveal that, during real-time flow simulation, the satellite-based rainfall products generally perform worse than the gauge-based rainfall. Moreover, as compared to the existing models, the flow forecasting by the WNARX model is way better than the other four models studied herein with the

  18. Activation of 112Cd by deuteron induced reactions up to 50 MeV: An alternative for 111In production?

    NASA Astrophysics Data System (ADS)

    Hermanne, A.; Adam Rebeles, R.; Van den Winkel, P.; Tárkányi, F.; Takács, S.

    2014-11-01

    In stacked foil irradiations with an incident 50 MeV deuteron beam on highly enriched 112Cd targets, the excitation functions for 109,110g,111In and 111,110g,106Ag were determined, relative to the monitor reactions natAl(d,x)22,24Na. The results were compared to the scarce literature values on enriched Cd isotopes. Through combination of reaction cross section data on all stable Cd isotopes listed in the on-line library TENDL-2013 (calculated with the TALYS 1.4 theoretical code) a comparison with our earlier study on natCd is made. The possible production of 111In through 112Cd(d,3n), as an alternative to 112Cd(p,2n), is discussed.

  19. Carbon footprint related to cattle production in Brazil, management practices and new alternatives.

    NASA Astrophysics Data System (ADS)

    de Figueiredo, Eduardo; de oliveira, Ricardo; Berchielli, Telma; Reis, Ricardo; La Scala, Newton

    2013-04-01

    Brazil has the World largest commercial beef cattle herd, over 209.5 million heads in 2010 and is the leading exports of cattle meat. It has been argued that this activity has an important impact on GHG emissions, but a variety of options exists for greenhouse gases (GHG) mitigation in agriculture. Among those, the most prominent options are associated to the improvement of crops and grazing land management. Our study is focused on the GHG balance related to the improvement of brachiaria spp. pasture, leading to increases in the animal stocking rate and meat production per area and time. This study is based on the IPCC (2006) methodology and others Brazil specific data and results presented by scientific literature to estimate GHG balance (emissions sources and sinks) for three scenarios proposed for brachiaria pasture: 1) degraded pasture, 2) managed pasture and 3) crop-livestock-forest integration system (CLFIS). The approach takes into account the amounts of supplies per hectare used for each of the simulated scenario projected over a 20 years period. The GHG estimates are presented in kg CO2eq per kg of liveweight, considering the following emission sources and sinks within farm-gate: i) CH4 from enteric fermentation, ii) CH4 from manure deposited on pasture, iii) N2O emissions from urine and dung deposited by cattle on pasture, iv) N2O emissions from N synthetic fertilizer, v) N2O emissions from crop residues as of N-fixing crops and pasture renewal returned to soils, vi) CO2 from potassium use, vii) CO2 from phosphorus use, viii) CO2 from insecticides use, ix) CO2 from herbicides use, x) CO2 emissions due to lime application, xi) emissions due to diesel combustion, xii) eucalyptus biomass sequestration and xiii) soil carbon sequestration. We considered initial body weight of 200 kg for each heifer and a final slaughter weight of 450 kg head-1 for all scenarios; for degraded pasture a stocking rate of 0,5 head ha-1 year-1 and liveweight gain of 83 kg head-1

  20. Real time pipelined system for forming the sum of products in the processing of video data

    NASA Technical Reports Server (NTRS)

    Wilcox, Brian (Inventor)

    1988-01-01

    A 3-by-3 convolver utilizes 9 binary arithmetic units connected in cascade for multiplying 12-bit binary pixel values P sub i which are positive or two's complement binary numbers by 5-bit magnitide (plus sign) weights W sub i which may be positive or negative. The weights are stored in registers including the sign bits. For a negative weight, the one's complement of the pixel value to be multiplied is formed at each unit by a bank of 17 exclusive or gates G sub i under control of the sign of the corresponding weight W sub i, and a correction is made by adding the sum of the absolute values of all the negative weights for each 3-by-3 kernel. Since this correction value remains constant as long as the weights are constant, it can be precomputed and stored in a register as a value to be added to the product PW of the first arithmetic unit.

  1. Lubricants based on renewable resources--an environmentally compatible alternative to mineral oil products.

    PubMed

    Willing, A

    2001-04-01

    The development of lubricants like, e.g. engine and hydraulic oils was traditionally based on mineral oil as a base fluid. This fact is related to the good technical properties and the reasonable price of mineral oils. The Report to the Club of Rome (W.W. Behrens III, D.H. Meadows, D.I. Meadows, J. Randers, The limits of growth, A Report to the Club of Rome, 1972) and the two oil crises of 1979 and 1983, however, elucidated that mineral oil is on principle a limited resource. In addition, environmental problems associated with the production and use of chemicals and the limited capacity of nature to tolerate pollution became obvious (G.H. Brundtland, et al., in: Hauff, Volker (Ed.), World Commission on Environment and Development (WCED), Report of the Brundtland-Commission, Oxford, UK, 1987), and the critical discussion included besides acid rain, smog, heavy metals, and pesticides also mineral oil (especially oil spills like the case Exxon Valdes). A disadvantage of mineral oil is its poor biodegradability and thus its potential for long-term pollution of the environment. From the early development of lubricants for special applications (e.g. turbojet engine oils) it was known, that fatty acid polyol esters have comparable or even better technical properties than mineral oil. Subsequently, innumerable synthetic esters have been synthesized by systematic variation of the fatty acid and the alcohol components. Whereas the alcohol moiety of the synthetic esters are usually of petrochemical origin, the fatty acids are almost exclusively based on renewable resources. The physico-chemical properties of oleochemical esters can cover the complete spectrum of technical requirements for the development of high-performance industrial oils and lubricants (e.g. excellent lubricating properties, good heat stability, high viscosity index, low volatility and superior shear stability). For a comprehensive review of their technical properties see F. Bongardt, in: Jahrbuchf

  2. MONTE GENEROSO ROCKFALL FIELD TEST (SWITZERLAND): Comparison between real rockfall volumes measurements and production calculations

    NASA Astrophysics Data System (ADS)

    Matasci, B.; Pedrazzini, A.; Humair, F.; Pedrozzi, G.; Carrea, D.; Loye, A.; Jaboyedoff, M.

    2012-04-01

    The Monte Generoso rockfall testing area is a mountainside located in the canton of Ticino (southern Switzerland) above an important highway that links Italy to Northern Europe. The slope is steep and consists of two high fractured limestone cliffs situated one above the other and divided by a sparse forest. The highway is potentially affected by rockfall hazard leading to the installation of several protective dams. The upper series of dams, collecting the blocks issued from the higher cliff, were emptied in May 2011. This gave the unique opportunity to assess the volumes of blocks produced in a known period of time in the different sections of the upper cliff. The cliff is formed by six catchments zones and the dams are therefore divided in six groups leading to the calculation of six volumes respectively. Based on geological and structural field data, a susceptibility assessment of the six portions of the cliff was performed and the results were compared to the six volume measured before emptying the dams. The aim is to spatially determine the main parameters influencing the rock fall production in the different portions of the cliff and to validate these results according to the material accumulated behind the protective dams. Structural analyses based on high resolution DEM and field investigation was performed to define the orientation and the geometrical characteristics of the discontinuity sets. The bedding plus five joint sets are present in the cliff and display a very small spacing. Multiples wedge structures affect the stability of the cliff and a surface parallel discontinuity set promotes the formation of flake instabilities. Based on a 1m cell-size DEM, the Matterocking method was applied in order to calculate the number of potential failure mechanisms (wedge and planar sliding) for each cell of the DEM. This allowed us to establish a first susceptibility rating for the six portions of the cliff. This rating was then refined by taking into account

  3. Internalizing production externalities: A structural estimation of real options in the upstream oil and gas industry

    NASA Astrophysics Data System (ADS)

    Muehlenbachs, Lucija

    There are hundreds of thousands of crude oil and natural gas wells across North America that are currently not producing oil or gas. Many of these wells have not been permanently decommissioned to meet environmental standards for permanent closure, but are in an inactive state that enables them to be more easily reactivated. Some of these wells have been in this inactive state for more than sixty years which begs the question of whether they will ever contribute to our energy supply, or whether they are being left inactive because the environmental remediation costs are prohibitively high. I estimate a structural model of optimal well operations over time and under uncertainty to determine what conditions or policies might push any of the inactive wells out of the hysteresis in which they reside. The model is further used to forecast production from existing wells and recoverable reserves from existing pools. The estimation uses data on production decisions from 84 thousand conventional oil and gas wells and estimates of the remaining reserves of 47 thousand pools. As the producer's decision depends on their subjective belief for how prices and recoverable reserves change over time, I also estimate the probability of changes in prices and recovery technology. I model increases and decreases in the estimated recoverable reserves to depend on price, and predict that natural gas reserves are more responsive to changes in price than conventional oil reserves. Under high prices there is potential for large increases in gas reserves, however this is not the case for oil reserves when the oil price is high. And likewise, under low prices, gas reserves decrease more than oil reserves. The dynamic programming model predicts that with only a drastic, arguably implausible, increase in prices and recovery rates will there be a significant increase in the number of inactive wells that are reactivated. If ideal conditions are not enough to induce well reactivation then this

  4. Cobalt products from real waste fractions of end of life lithium ion batteries.

    PubMed

    Pagnanelli, Francesca; Moscardini, Emanuela; Altimari, Pietro; Abo Atia, Thomas; Toro, Luigi

    2016-05-01

    An innovative process was optimized to recover Co from portable Lithium Ion Batteries (LIB). Pilot scale physical pretreatment was performed to recover electrodic powder from LIB. Co was extracted from electrodic powder by a hydrometallurgical process including the following main stages: leaching (by acid reducing conditions), primary purification (by precipitation of metal impurities), solvent extraction with D2EPHA (for removal of metal impurities), solvent extraction with Cyanex 272 (for separation of cobalt from nickel), cobalt recovery (by precipitation of cobalt carbonate). Tests were separately performed to identify the optimal operating conditions for precipitation (pH 3.8 or 4.8), solvent extraction with D2EHPA (pH 3.8; Mn/D2EHPA=4; 10% TBP; two sequential extractive steps) and solvent extraction with Cyanex 272 (pH 3.8; Cyanex/Cobalt=4, 10% TBP, one extractive step). The sequence of optimized process stages was finally performed to obtain cobalt carbonate. Products with different degree of purity were obtained depending on the performed purification steps (precipitation with or without solvent extraction). 95% purity was achieved by implementation of the process including the solvent extraction stages with D2EHPA and Cyanex 272 and final washing for sodium removal. PMID:26564258

  5. Communication: Real time observation of unimolecular decay of Criegee intermediates to OH radical products

    NASA Astrophysics Data System (ADS)

    Fang, Yi; Liu, Fang; Barber, Victoria P.; Klippenstein, Stephen J.; McCoy, Anne B.; Lester, Marsha I.

    2016-02-01

    In the atmosphere, a dominant loss process for carbonyl oxide intermediates produced from alkene ozonolysis is also an important source of hydroxyl radicals. The rate of appearance of OH radicals is revealed through direct time-domain measurements following vibrational activation of prototypical methyl-substituted Criegee intermediates under collision-free conditions. Complementary theoretical calculations predict the unimolecular decay rate for the Criegee intermediates in the vicinity of the barrier for 1,4 hydrogen transfer that leads to OH products. Both experiment and theory yield unimolecular decay rates of ca. 108 and 107 s-1 for syn-CH3CHOO and (CH3)2COO, respectively, at energies near the barrier. Tunneling through the barrier, computed from high level electronic structure theory and experimentally validated, makes a significant contribution to the decay rate. Extension to thermally averaged unimolecular decay of stabilized Criegee intermediates under atmospheric conditions yields rates that are six orders of magnitude slower than those evaluated directly in the barrier region.

  6. Communication: Real time observation of unimolecular decay of Criegee intermediates to OH radical products.

    PubMed

    Fang, Yi; Liu, Fang; Barber, Victoria P; Klippenstein, Stephen J; McCoy, Anne B; Lester, Marsha I

    2016-02-14

    In the atmosphere, a dominant loss process for carbonyl oxide intermediates produced from alkene ozonolysis is also an important source of hydroxyl radicals. The rate of appearance of OH radicals is revealed through direct time-domain measurements following vibrational activation of prototypical methyl-substituted Criegee intermediates under collision-free conditions. Complementary theoretical calculations predict the unimolecular decay rate for the Criegee intermediates in the vicinity of the barrier for 1,4 hydrogen transfer that leads to OH products. Both experiment and theory yield unimolecular decay rates of ca. 10(8) and 10(7) s(-1) for syn-CH3CHOO and (CH3)2COO, respectively, at energies near the barrier. Tunneling through the barrier, computed from high level electronic structure theory and experimentally validated, makes a significant contribution to the decay rate. Extension to thermally averaged unimolecular decay of stabilized Criegee intermediates under atmospheric conditions yields rates that are six orders of magnitude slower than those evaluated directly in the barrier region. PMID:26874475

  7. Alternative Acetate Production Pathways in Chlamydomonas reinhardtii during Dark Anoxia and the Dominant Role of Chloroplasts in Fermentative Acetate Production[W

    PubMed Central

    Catalanotti, Claudia; D’Adamo, Sarah; Wittkopp, Tyler M.; Ingram-Smith, Cheryl J.; Mackinder, Luke; Miller, Tarryn E.; Heuberger, Adam L.; Peers, Graham; Smith, Kerry S.; Jonikas, Martin C.; Grossman, Arthur R.; Posewitz, Matthew C.

    2014-01-01

    Chlamydomonas reinhardtii insertion mutants disrupted for genes encoding acetate kinases (EC 2.7.2.1) (ACK1 and ACK2) and a phosphate acetyltransferase (EC 2.3.1.8) (PAT2, but not PAT1) were isolated to characterize fermentative acetate production. ACK1 and PAT2 were localized to chloroplasts, while ACK2 and PAT1 were shown to be in mitochondria. Characterization of the mutants showed that PAT2 and ACK1 activity in chloroplasts plays a dominant role (relative to ACK2 and PAT1 in mitochondria) in producing acetate under dark, anoxic conditions and, surprisingly, also suggested that Chlamydomonas has other pathways that generate acetate in the absence of ACK activity. We identified a number of proteins associated with alternative pathways for acetate production that are encoded on the Chlamydomonas genome. Furthermore, we observed that only modest alterations in the accumulation of fermentative products occurred in the ack1, ack2, and ack1 ack2 mutants, which contrasts with the substantial metabolite alterations described in strains devoid of other key fermentation enzymes. PMID:25381350

  8. Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation

    PubMed Central

    Wisnewski, Adam V.; Liu, Jian; Colangelo, Christopher M.

    2015-01-01

    Isocyanates have been a leading chemical cause of occupational asthma since their utility for generating polyurethane was first recognized over 60 years ago, yet the mechanisms of isocyanate asthma pathogenesis remain unclear. The present study provides in vivo evidence that a GSH mediated pathway underlies asthma-like eosinophilic inflammatory responses to respiratory tract isocyanate exposure. In naïve mice, a mixture of GSH reaction products with the chemical allergen, methylene-diphenyl diisocyanate (MDI), induced innate immune responses, characterized by significantly increased airway levels of Chitinase YM-1 and IL-12/IL-23β (but not α) subunit. However, in mice immunologically sensitized to MDI via prior skin exposure, identical GSH–MDI doses induced substantially greater inflammatory responses, including significantly increased airway eosinophil numbers and mucus production, along with IL-12/IL-23β, chitinases, and other indicators of alternative macrophage activation. The “self”-protein albumin in mouse airway fluid was uniquely modified by GSH–MDI at position 414K, a preferred site of MDI reactivity on human albumin. The 414K–MDI conjugation appears to covalently cross-link GSH to albumin via GSH's NH2-terminus, a unique conformation possibly resulting from cyclized mono(GSH)–MDI or asymmetric (S,N′-linked) bis(GSH)–MDI conjugates. Together, the data support a possible thiol mediated transcarbamoylating mechanism linking MDI exposure to pathogenic eosinophilic inflammatory responses. PMID:25635619

  9. A sub-minute CZE method to determine nitrate and nitrite in meat products: An alternative for routine analysis.

    PubMed

    Della Betta, Fabiana; Pereira, Lais Morilla; Siqueira, Mariana Araújo; Valese, Andressa Camargo; Daguer, Heitor; Fett, Roseane; Vitali, Luciano; Costa, Ana Carolina Oliveira

    2016-09-01

    A sub-minute capillary zone electrophoresis (CZE) method was optimized and a simple sample preparation procedure based on the extraction of the analytes with water and sodium tetraborate was developed for the simultaneous determination of nitrate and nitrite levels in meat products. The background electrolyte (BGE) was composed of 20mmolL(-1) perchloric acid and 65mmolL(-1) β-alanine at pH3.83. Thiocyanate was used as the internal standard. The proposed method was validated and the uncertainty estimated according to Eurachem guidelines. The run time was only 30s, allowing analyzing more than 25samples/h, the good analytical performance confirms the suitability of the method for the analysis of meat products. One sample presented residual nitrite levels above the limit established by MERCOSUL legislation (150mgkg(-1)). The use of a fast method in association with a simple sample preparation step means that this procedure represents a possible alternative to fulfill the demand for high throughput in routine laboratory analysis. PMID:27132205

  10. Renewable sugars from oil palm frond juice as an alternative novel fermentation feedstock for value-added products.

    PubMed

    Zahari, Mior Ahmad Khushairi Mohd; Zakaria, Mohd Rafein; Ariffin, Hidayah; Mokhtar, Mohd Noriznan; Salihon, Jailani; Shirai, Yoshihito; Hassan, Mohd Ali

    2012-04-01

    In this paper, we report that pressed juice from oil palm frond (OPF) contained renewable sugars such as glucose, sucrose and fructose. By using a simple sugarcane press, 50% (wt/wt) of OPF juice was obtained from fresh OPF. The glucose content in the juice was 53.95±2.86g/l, which accounts for 70% of the total free sugars. We have examined the effect of various OPF juice concentrations on the production of poly(3-hydroxybutyrate), P(3HB) by Cupriavidus necator CCUG 52238(T). The cell dry mass in shake flask experiment reached 8.42g/l, with 32wt.% of P(3HB) at 30% (v/v) of OPF juice, comparable with using technical grade sugars. The biopolymer had a molecular mass, M(w) of 812kDa, with a low polydispersity index of 1.61. This result indicates that OPF juice can be used as an alternative renewable carbon source for P(3HB) production and has potential as a renewable carbon source. PMID:22342083

  11. Contribution of Near Real Time MODIS-Based Forest Disturbance Detection Products to a National Forest Threat Early Warning System

    NASA Technical Reports Server (NTRS)

    Spruce, Joseph P.; Hargrove, William; Glasser, Jerry; Kuper, Philip D.

    2011-01-01

    This presentation discusses an effort to compute and post weekly MODIS forest change products for the conterminous US (CONUS), as part of national forest threat early warning system (EWS) known as the U.S. Forest Change Assessment Viewer (FCAV). The US Forest Service, NASA, USGS, and ORNL are working collaboratively to contribute weekly change products to this EWS. Large acreages of the nation's forests are being disturbed by a growing multitude of biotic and abiotic threats that can act either singularly or in combination. When common at regional scales, such disturbances can pose hazards and threats to floral and faunal bio-diversity, ecosystem sustainability, ecosystem services, and human settlements across the conterminous US. Regionally evident forest disturbances range from ephemeral periodic canopy defoliation to stand replacement mortality events due to insects, disease, fire, hurricanes, tornadoes, ice, hail, and drought. Mandated by the Healthy Forest Restoration Act of 2003, this forest threat EWS has been actively developed since 2006 and on-line since 2010. This FCAV system employs 250-meter MODIS NDVI-based forest change products as a key element of the system, providing regional and CONUS scale products in near real time every 8 days. Each forest change product in FCAV is based on current versus historical 24 day composite NDVI data gridded at 231.66 meter resolution. Current NDVI is derived from USGS eMODIS expedited products. MOD13 NDVI is used for constructing historical baselines. CONUS change products are computed for all forests as % change in the current versus historical NDVI. Change products are computed according to previous year, previous 3 years and previous 8 year historical baselines. The use of multiple baselines enables disturbance anomaly phenology to be more fully assessed. CONUS forest change products are posted each week on the FCAV, a web mapping service maintained by the National Environmental Modeling and Analysis Center. The

  12. Contribution of Near Real Time MODIS-Based Forest Disturbance Detection Products to a National Forest Threat Early Warning System

    NASA Astrophysics Data System (ADS)

    Spruce, J.; Hargrove, W. W.; Gasser, J.; Smoot, J.; Kuper, P.

    2011-12-01

    This presentation discusses an effort to compute and post weekly MODIS forest change products for the conterminous US (CONUS), as part of a web-based national forest threat early warning system (EWS) known as the U.S. Forest Change Assessment Viewer (FCAV). The US Forest Service, NASA, USGS, and ORNL are working collaboratively to contribute weekly change products to this EWS. Large acreages of the nation's forests are being disturbed by a growing multitude of biotic and abiotic threats that can act either singularly or in combination. When common at regional scales, such disturbances can pose hazards and threats to floral and faunal bio-diversity, ecosystem sustainability, ecosystem services, and human settlements across the conterminous US. Regionally evident forest disturbances range from ephemeral periodic canopy defoliation to stand replacement mortality events due to insects, disease, fire, hurricanes, tornadoes, ice, hail, and drought. Mandated by the Healthy Forest Restoration Act of 2003, this forest threat EWS has been actively developed since 2006 and on-line since 2010. The FCAV system employs 250-meter MODIS NDVI-based forest change products as a key element of the system, providing regional and CONUS scale products in near real time every 8 days. Each of our forest change products in FCAV is based on current versus historical 24 day composites of NDVI data gridded at 231.66 meter resolution. Current NDVI is derived from USGS eMODIS expedited products. MOD13 NDVI is used for constructing historical baselines. CONUS change products are computed for all forests as % change in the current versus historical NDVI for a given 24 day period. Change products are computed according to previous year, previous 3 year and previous 8 year historical baselines. The use of multiple baselines enables apparent forest disturbance anomalies to be more fully assessed. CONUS forest change products are posted each week on the FCAV, a web mapping service constructed and

  13. Multicriteria cost–benefit assessment of tannery production: The need for breakthrough process alternatives beyond conventional technology optimization

    SciTech Connect

    Giannetti, Biagio F.; Agostinho, Feni; Moraes, Luciano C.; Almeida, Cecília M.V.B.; Ulgiati, Sergio

    2015-09-15

    The worldwide use of chromium-based processes in tanneries generates increased concerns about their related environmental burdens. Cleaner production alternatives for leather production are being proposed, based on the optimization of specific aspects or criteria, for instance, reducing demand for specific materials and energy, or reducing local toxicological emissions. While improvement on individual characteristics of the process is certainly to be favored, a more comprehensive evaluation of alternatives is also needed to prevent the risk of shifting the burden to increase global load while addressing one specific critical factor of production. This work aims to discuss the importance of a multicriteria, multiscale approach to address cleaner production strategy costs and benefits. For this, materials balance, an economic approach, and emergy (with an “m”) accounting methods are applied to selected unhairing/liming, pickling/tanning and wastewater treatment steps in a tannery process, which was chosen as a case study. Results show that the assessed recycling cleaner production strategies assessed allow the manufacturer to reduce by one half the amount of water used and the demand for chemicals up to 4% with respect to the business-as-usual process, at the expense of increasing electricity demand by 10%. Economic cost-to-benefit ratio was 25$ benefits per 1$ invested, as well as an emergy-based cost-to-benefit of 33Em$ per 1Em$ invested, of course these improvements were limited to the three investigated process steps. The improvement in cost/benefit ratios indicates that converting scenario #0 into #1 is favorable under economic and emergy views. However, when the two scenarios are investigated from the point of view of the imbalance in local and renewable resource use versus imported and nonrenewable use, the emergy method shows a small overall increase in renewability (from 3.51% to 3.85%), a low, but expected, emergy yield ratio equal to 1, and a high

  14. Transverse momentum dependent parton densities in associated real and virtual photon and jet production at the LHC

    NASA Astrophysics Data System (ADS)

    Lipatov, A. V.; Zotov, N. P.

    2014-11-01

    We study the associated production of real (isolated) or virtual photons (with their subsequent leptonic decay) and hadronic jets in proton-proton collisions at the LHC using the kT-factorization approach of QCD. The consideration is based on the off-shell quark-gluon QCD Compton scattering subprocesses. In the case of virtual photon production, the contributions from Z boson exchange as well as γ*-Z interference with the full spin correlations are included. The transversemomentum-dependent (TMD) quark and gluon densities in a proton are determined from the Kimber-Martin-Ryskin prescription or Catani-Ciafoloni-Fiorani-Marchesini (CCFM) equation. In the latter, we restrict to the case where the gluon-to-quark splitting occurs at the last evolution step and calculate the sea quark density as a convolution of the CCFM-evolved gluon distribution and the TMD gluon-to-quark splitting function. Our numerical predictions are compared with the recent experimental data taken by the ATLAS Collaboration. We discuss the theoretical uncertainties of our calculations and argue that further studies are capable of constraining the TMD parton densities in the proton.

  15. Production of high concentrations of H2O2 in a bioelectrochemical reactor fed with real municipal wastewater.

    PubMed

    Modin, Oskar; Fukushi, Kensuke

    2013-01-01

    Bioelectrochemical systems can be used to energy-efficiently produce hydrogen peroxide (H2O2) from wastewater. Organic compounds in the wastewater are oxidized by microorganisms using the anode as electron acceptor. H2O2 is produced by reduction of oxygen on the cathode. In this study, we demonstrate for the first time production of high concentrations of H2O2 production from real municipal wastewater. A concentration of 2.26 g/L H2O2 was produced in 9 h at 8.3 kWh/kgH2O2. This concentration could potentially be useful for membrane cleaning at membrane bioreactor wastewater treatment plants. With an acetate-containing nutrient medium as anode feed, a H2O2 concentration of 9.67 g/L was produced in 21 h at an energy cost of 3.0 kWh/kgH2O2. The bioelectrochemical reactor used in this study suffered from a high internal resistance, most likely caused by calcium carbonate deposits on the cathode-facing side of the cation exchange membrane separating the anode and cathode compartments. PMID:24527636

  16. Dempster-Shafer theory applied to regulatory decision process for selecting safer alternatives to toxic chemicals in consumer products.

    PubMed

    Park, Sung Jin; Ogunseitan, Oladele A; Lejano, Raul P

    2014-01-01

    Regulatory agencies often face a dilemma when regulating chemicals in consumer products-namely, that of making decisions in the face of multiple, and sometimes conflicting, lines of evidence. We present an integrative approach for dealing with uncertainty and multiple pieces of evidence in toxics regulation. The integrative risk analytic framework is grounded in the Dempster-Shafer (D-S) theory that allows the analyst to combine multiple pieces of evidence and judgments from independent sources of information. We apply the integrative approach to the comparative risk assessment of bisphenol-A (BPA)-based polycarbonate and the functionally equivalent alternative, Eastman Tritan copolyester (ETC). Our results show that according to cumulative empirical evidence, the estimated probability of toxicity of BPA is 0.034, whereas the toxicity probability for ETC is 0.097. However, when we combine extant evidence with strength of confidence in the source (or expert judgment), we are guided by a richer interval measure, (Bel(t), Pl(t)). With the D-S derived measure, we arrive at various intervals for BPA, with the low-range estimate at (0.034, 0.250), and (0.097,0.688) for ETC. These new measures allow a reasonable basis for comparison and a justifiable procedure for decision making that takes advantage of multiple sources of evidence. Through the application of D-S theory to toxicity risk assessment, we show how a multiplicity of scientific evidence can be converted into a unified risk estimate, and how this information can be effectively used for comparative assessments to select potentially less toxic alternative chemicals. PMID:23804574

  17. Use of ASCAT derived soil moisture product for real-time flood forecasting in the Upper Tiber River

    NASA Astrophysics Data System (ADS)

    Brocca, Luca; Melone, Florisa; Moramarco, Tommaso; Wagner, Wolfgang; Hasenauer, Stefan; Berni, Nicola

    2010-05-01

    The role and the importance of soil moisture for meteorological, agricultural and hydrological applications is widely acknowledged. In particular, for a given storm event, different values of initial soil moisture conditions can discriminate between minor or catastrophic effects. Therefore, a real time flood forecasting system founded on a rainfall-runoff model strictly requires an accurate estimation of the initial state of the catchment wetness to obtain a reliable estimation of the flood hydrograph. It has to be pointed out that, in flood-prone areas, a Flood Monitoring and Warning System operating in real time represents the main non-structural measure to be actuated to dampen the risk. At the catchment scale, soil moisture monitoring can be addressed by using sensors operating on remote sensing platforms. Among them, the coarse resolution scatterometers have been employed in different studies due to their high temporal resolution suitable for hydrological applications. Specifically, the Advanced Scatterometer (ASCAT) on-board of the Meteorological Operational satellite provides an operative surface soil moisture product available at global scale since March 2007. This sensor is characterized by a spatial resolution of 25/50 km and a nearly daily time step. To get profile soil moisture estimates, an exponential filter is applied to the time series of the ASCAT surface soil moisture obtaining the so-called Soil Wetness Index (SWI). The reliability of the SWI was recently evaluated through the comparison both with in-situ and modelled soil moisture data in the Upper Tiber River basin. In this study, the effects of assimilating satellite-derived soil moisture estimates into a continuous and distributed rainfall-runoff model, named MISDc, were assessed. This topic is relevant not only for scientific purposes but also for operational applications. In fact, the MISDc model is actually operative at the Umbria Region Functional Centre for real time flood forecasting in

  18. Sequencing of two alternatively spliced mRNAs corresponding to the extracellular domain of the rat receptor for advanced glycosylation end products (RAGE).

    PubMed

    Girón, M D; Vargas, A M; Suárez, M D; Salto, R

    1998-10-01

    The receptor for advanced glycosylation end products (RAGE) is an integral membrane protein responsible for the recognition and internalization of those extensively modified proteins. The receptor has an extracellular domain that binds to the advanced glycosylation end products. By reverse-transcription and polymerase chain reaction amplification, we have identified in rat liver and kidney two amplified products that correspond to cDNA coding for a part of the extracellular domain of the receptor. Sequencing of these products showed that these amplified molecules were similar except for a 27-bp fragment that was absent in the smaller product. This spliced region is located close to the transmembrane region of the receptor. We have confirmed the possibility of the alternative splicing in the generation of these mRNA isoforms by cloning a fragment of the rat gene for RAGE. This fragment has a distribution of introns and exons fully compatible with the proposed alternative splicing. PMID:9790936

  19. Alternative models of climatic effects on sockeye salmon (Oncorhynchus nerka) productivity in Bristol Bay, Alaska, and the Fraser River, British Columbia

    USGS Publications Warehouse

    Adkison, M.; Peterman, R.; Lapointe, M.; Gillis, D.; Korman, J.

    1996-01-01

    We compare alternative models of sockeye salmon (Oncorhynchus nerka) productivity (returns per spawner) using more than 30 years of catch and escapement data for Bristol Bay, Alaska, and the Fraser River, British Columbia. The models examined include several alternative forms of models that incorporate climatic influences as well as models not based on climate. For most stocks, a stationary stock-recruitment relationship explains very little of the interannual variation in productivity. In Bristol Bay, productivity co-varies among stocks and appears to be strongly related to fluctuations in climate. The best model for Bristol Bay sockeye involved a change in the 1970s in the parameters of the Ricker stock-recruitment curve; the stocks generally became more productive. In contrast, none of the models of Fraser River stocks that we examined explained much of the variability in their productivity.

  20. Splenic RNA and MicroRNA Mimics Promote Complement Factor B Production and Alternative Pathway Activation via Innate Immune Signaling.

    PubMed

    Zou, Lin; Feng, Yan; Xu, Ganqiong; Jian, Wenling; Chao, Wei

    2016-03-15

    Complement factor B (cfB) is an essential component of the alternative pathway (AP) and plays an important role in the pathogenesis of polymicrobial sepsis. However, the mechanism leading to cfB production and AP activation during sepsis remains poorly understood. In this study, we found that plasma cell-free RNA was significantly increased following cecal ligation and puncture (CLP), an animal model of polymicrobial sepsis, and was closely associated with sepsis severity. Quantitative RT-PCR and microRNA (miRNA) array analysis revealed an increase in bacterial RNA and multiple host miRNAs (miR-145, miR-146a, miR-122, miR-210) in the blood following CLP. Treatment with tissue RNA or synthetic miRNA mimics (miR-145, miR-146a, miR-122, miR-34a) induced a marked increase in cfB production in cardiomyocytes or macrophages. The newly synthesized cfB released into medium was biologically active because it participated in AP activation initiated by cobra venom factor. Genetic deletion of TLR7 or MyD88, but not TLR3, and inhibition of the MAPKs (JNK and p38) or NF-κB abolished miR-146a-induced cfB production. In vivo, CLP led to a significant increase in splenic cfB expression that correlated with the plasma RNA or miRNA levels. Peritoneal injection of RNA or miR-146a led to an increase in cfB expression in the peritoneal space that was attenuated in MyD88-knockout or TLR7-knockout mice, respectively. These findings demonstrate that host cellular RNA and specific miRNAs are released into the circulation during polymicrobial sepsis and may function as extracellular mediators capable of promoting cfB production and AP activation through specific TLR7 and MyD88 signaling. PMID:26889043

  1. Alternative public-policy incentives and the development of ethanol-production facilities in New York state. Final report

    SciTech Connect

    Not Available

    1981-12-01

    The federal and state incentives available to investors are outlined and the impact of selected incentives on ethanol plant feasibility in New York state is assessed. Two ethanol plants are selected as representative facilities against which the various incentive programs can be evaluated. One plant has the annual capacity to produce 1.675 million gallons from deproteinized cheese whey. The second plant has an annual capacity of 25 million gallons from corn grain. These two plants represent significantly different options to potential plant developers. The cheese whey-based plant is a relatively low-volume facility, but utilizes a low-cost waste product of the dairy sector. The corn-based plant is a high-volume enterprise, but utilizes corn, which has many alternative uses to ethanol production and is thus a potentially expensive feedstock. Neither plant necessarily represents an optimal scale of conversion for its respective feedstock. However, each plant is well suited for demonstrating the impacts of incentive programs. Of the various incentives tested, the federal fuel excise tax exemption appears to be the most important, followed by the Federal Energy Investment Tax Credit, state industrial revenue bond financing, and the Accelerated Capital Recovery System provisions of the Economic Recovery Tax Act of 1981. The incentives studied are successful in reducing, to various degrees, the uncertainty surrounding investments in ethanol fuels. However, it would appear that the incentives cannot compensate for a lack of basic economic strength. The whey-to-ethanol plant is relatively risk-free under a wide range of assumptions even when no incentives are utilized.

  2. Sustainable, alternative farming practices as a means to simultaneously secure food production and reduce air pollution in East Asia

    NASA Astrophysics Data System (ADS)

    Tai, A. P. K.; Fung, K. M.; Yong, T.; Liu, X.

    2015-12-01

    Proper agricultural land management is essential for securing food supply and minimizing damage to the environment. Among available farming practices, relay strip intercropping and fertilizer application are commonly used, but to study their wider environmental implications and possible feedbacks we require an Earth system modeling framework. In this study, the effectiveness of a maize-soybean relay strip intercropping system and fertilizer reduction is investigated using a multi-model method. The DNDC (DeNitrification-DeComposition) model is used to simulate agricultural activities and their impacts on the environment through nitrogen emissions and changes in soil chemical composition. Crop yield, soil nutrient content and nitrogen emissions to the atmosphere in major agricultural regions of China are predicted under various cultivation scenarios. The GEOS-Chem global chemical transport model is then used to estimate the effects on downwind particle and ozone air pollution. We show that relay strip intercropping and optimal fertilization not only improve crop productivity, but also retain soil nutrients, reduce ammonia emission and mitigate downwind air pollution. By cutting 25% fertilization inputs but cultivating maize and soybean together in a relay strip intercropping system used with field studies, total crop production was improved slightly by 4.4% compared to monoculture with conventional amount of fertilizers. NH3 volatilization decreases by 29%, equivalent to saving the pollution-induced health damage costs by about US$2.5 billion per year. The possible feedback effects from atmospheric nitrogen deposition onto the croplands are also investigated. We show that careful management and better quantitative understanding of alternative farming practices hold huge potential in simultaneously addressing different global change issues including the food crisis, air pollution and climate change, and calls for greater collaboration between scientists, farmers and

  3. Production, Characterization and Biocompatibility of Marine Collagen Matrices from an Alternative and Sustainable Source: The Sea Urchin Paracentrotus lividus

    PubMed Central

    Di Benedetto, Cristiano; Barbaglio, Alice; Martinello, Tiziana; Alongi, Valentina; Fassini, Dario; Cullorà, Emanuele; Patruno, Marco; Bonasoro, Francesco; Barbosa, Mario Adolfo; Candia Carnevali, Maria Daniela; Sugni, Michela

    2014-01-01

    Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is required, and marine organisms are among the safest and recently exploited ones. Sea urchins possess a circular area of soft tissue surrounding the mouth, the peristomial membrane (PM), mainly composed by mammalian-like collagen. The PM of the edible sea urchin Paracentrotus lividus therefore represents a potential unexploited collagen source, easily obtainable as a food industry waste product. Our results demonstrate that it is possible to extract native collagen fibrils from the PM and produce suitable substrates for in vitro system. The obtained matrices appear as a homogeneous fibrillar network (mean fibril diameter 30–400 nm and mesh < 2 μm) and display remarkable mechanical properties in term of stiffness (146 ± 48 MPa) and viscosity (60.98 ± 52.07 GPa·s). In vitro tests with horse pbMSC show a good biocompatibility in terms of overall cell growth. The obtained results indicate that the sea urchin P. lividus can be a valuable low-cost collagen source for mechanically resistant biomedical devices. PMID:25255130

  4. Production, characterization and biocompatibility of marine collagen matrices from an alternative and sustainable source: the sea urchin Paracentrotus lividus.

    PubMed

    Benedetto, Cristiano Di; Barbaglio, Alice; Martinello, Tiziana; Alongi, Valentina; Fassini, Dario; Cullorà, Emanuele; Patruno, Marco; Bonasoro, Francesco; Barbosa, Mario Adolfo; Carnevali, Maria Daniela Candia; Sugni, Michela

    2014-09-01

    Collagen has become a key-molecule in cell culture studies and in the tissue engineering field. Industrially, the principal sources of collagen are calf skin and bones which, however, could be associated to risks of serious disease transmission. In fact, collagen derived from alternative and riskless sources is required, and marine organisms are among the safest and recently exploited ones. Sea urchins possess a circular area of soft tissue surrounding the mouth, the peristomial membrane (PM), mainly composed by mammalian-like collagen. The PM of the edible sea urchin Paracentrotus lividus therefore represents a potential unexploited collagen source, easily obtainable as a food industry waste product. Our results demonstrate that it is possible to extract native collagen fibrils from the PM and produce suitable substrates for in vitro system. The obtained matrices appear as a homogeneous fibrillar network (mean fibril diameter 30-400 nm and mesh < 2 μm) and display remarkable mechanical properties in term of stiffness (146 ± 48 MPa) and viscosity (60.98 ± 52.07 GPa·s). In vitro tests with horse pbMSC show a good biocompatibility in terms of overall cell growth. The obtained results indicate that the sea urchin P. lividus can be a valuable low-cost collagen source for mechanically resistant biomedical devices. PMID:25255130

  5. Biomethane Production as an Alternative Bioenergy Source from Codigesters Treating Municipal Sludge and Organic Fraction of Municipal Solid Wastes

    PubMed Central

    Ersahin, M. Evren; Yangin Gomec, Cigdem; Dereli, R. Kaan; Arikan, Osman; Ozturk, Izzet

    2011-01-01

    Energy recovery potential of a mesophilic co-digester treating OFMSW and primary sludge at an integrated biomethanization plant was investigated based on feasibility study results. Since landfilling is still the main solid waste disposal method in Turkey, land scarcity will become one of the most important obstacles. Restrictions for biodegradable waste disposal to sanitary landfills in EU Landfill Directive and uncontrolled long-term contamination with gas emissions and leachate necessitate alternative management strategies due to rapid increase in MSW production. Moreover, since energy contribution from renewable resources will be required more in the future with increasing oil prices and dwindling supplies of conventional energy sources, the significance of biogas as a renewable fuel has been increased in the last decade. Results indicated that almost 93% of annual total cost can be recovered if 100% renewable energy subsidy is implemented. Besides, considering the potential revenue when replacing transport fuels, about 26 heavy good vehicles or 549 cars may be powered per year by the biogas produced from the proposed biomethanization plant (PE = 100,000; XPS = 61 g TS/PE·day; XSS-OFMSW = 50 g TS/PE·day). PMID:21274432

  6. Replacing monocultures with mixed-species stands: Ecosystem service implications of two production forest alternatives in Sweden.

    PubMed

    Felton, Adam; Nilsson, Urban; Sonesson, Johan; Felton, Annika M; Roberge, Jean-Michel; Ranius, Thomas; Ahlström, Martin; Bergh, Johan; Björkman, Christer; Boberg, Johanna; Drössler, Lars; Fahlvik, Nils; Gong, Peichen; Holmström, Emma; Keskitalo, E Carina H; Klapwijk, Maartje J; Laudon, Hjalmar; Lundmark, Tomas; Niklasson, Mats; Nordin, Annika; Pettersson, Maria; Stenlid, Jan; Sténs, Anna; Wallertz, Kristina

    2016-02-01

    Whereas there is evidence that mixed-species approaches to production forestry in general can provide positive outcomes relative to monocultures, it is less clear to what extent multiple benefits can be derived from specific mixed-species alternatives. To provide such insights requires evaluations of an encompassing suite of ecosystem services, biodiversity, and forest management considerations provided by specific mixtures and monocultures within a region. Here, we conduct such an assessment in Sweden by contrasting even-aged Norway spruce (Picea abies)-dominated stands, with mixed-species stands of spruce and birch (Betula pendula or B. pubescens), or spruce and Scots pine (Pinus sylvestris). By synthesizing the available evidence, we identify positive outcomes from mixtures including increased biodiversity, water quality, esthetic and recreational values, as well as reduced stand vulnerability to pest and pathogen damage. However, some uncertainties and risks were projected to increase, highlighting the importance of conducting comprehensive interdisciplinary evaluations when assessing the pros and cons of mixtures. PMID:26744048

  7. Quantitative detection of pork in commercial meat products by TaqMan® real-time PCR assay targeting the mitochondrial D-loop region.

    PubMed

    Kim, Miju; Yoo, Insuk; Lee, Shin-Young; Hong, Yeun; Kim, Hae-Yeong

    2016-11-01

    The TaqMan® real-time PCR assay using the mitochondrial D-loop region was developed for the quantitative detection of pork in processed meat products. The newly designed primers and probe specifically amplified pork without any cross-reactivity with non-target animal species. The limit of detection of the real-time PCR assay was 0.1pg of heat-treated pork meat and 0.1% (w/w) pork meat in beef and chicken meat mixtures. The quantitative real-time PCR assay was applied to analyze the pork meat content in 22 commercial processed meat products including jerkies, press hams, sausages, hamburger patties and steaks, grilled short rib patties, and nuggets. The developed real-time PCR method was able to detect pork meat in various types of processed meat products that declared the use of pork meat on their label. All processed meat products that declared no use of pork meat showed a negative result in the assay. The method developed in this study showed sensitivity and specificity in the quantification of pork meat in commercial processed meat products. PMID:27211626

  8. The Real Role of N-Heterocyclic Carbene in Reductive Functionalization of CO2: An Alternative Understanding from Density Functional Theory Study.

    PubMed

    Zhou, Qinghai; Li, Yuxue

    2015-08-19

    The mechanisms of reductive functionalization of CO2 to formamide catalyzed by N-heterocyclic carbene (NHC) were comprehensively studied with DFT calculations. New activation mode with much lower energy barrier than those proposed before was discovered. In this reaction, NHC acts as neither a CO2 nor a silane activator, but as a precursor of the real catalyst, i.e., the in situ formed ionic liquid [NHCH](+)[Carbamate](-). In this loose contact ion pair, the negatively charged O atom of the carbamate anion becomes the new active site and is free to do nucleophilic attack. When amine is absent, CO2 will be converted into methanol. In this case, the NHC-CO2 adduct is the real catalytic species, the active site shifted from the carbene C atom to the negatively charged O atom. These new activation modes follow a pattern of "S(N)2@Si-Acceptor", in which the Si-H bond is activated via concerted backside S(N)2 nucleophilic attack by the negatively charged O atom, and the leaving hydride is directly accepted by a free CO2 molecule. The advantages of these new activation modes originate from the following points: (1) The ionic liquid [NHCH](+)[Carbamate](-) and NHC-CO2 adduct are thermodynamically more stable than NHC. (2) The active site of the NHC catalyst is extended outside a lot. Consequently, the large steric effect between the NHC arms and the substrates in transition state can be avoided to some extent. (3) The O atom has good silicon affinity. In addition, a free CO2 molecule, whose carbon atom is more electrophilic than those of the CO2 moieties in NHC-CO2 adduct and carbamate, acts as an efficient hydride acceptor. PMID:26220202

  9. Simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products using real time PCR-melt curve analysis.

    PubMed

    Singh, Jitender; Batish, Virender K; Grover, Sunita

    2012-04-01

    The present investigation reports development of post real time PCR (RTi-PCR) - melt curve analysis for simultaneous detection of Listeria monocytogenes and Salmonella spp. The optimal Sybr Green I (SG-I) concentration of 1.6 μM resulted in two specific peaks with melting temperature (Tm) of 79.90 ± 0.39 °C and 86.29 ± 0.13 °C for L. monocytogenes and Salmonella spp respectively. The detection sensitivity of the assay in reconstituted non-fat dried milk (NFDM; 11%) spiked with the target pathogens at different levels was 3 log cfu per ml of each pathogen. However, the sensitivity was improved up to 1 log cfu per ml by including pre-enrichment step of 6 h. On application of assay on 60 market samples, one sample each of raw milk and ice cream was detected positive for L. monocytogenes and Salmonella spp. Assay was quite specific as no cross reactivity with non target cultures could be observed. The developed assay can find valuable application in monitoring dairy products for the presence of L. monocytogenes and Salmonella spp. to ensure their microbiological quality and safety. PMID:23572847

  10. An insight of disinfection by-product (DBP) formation by alternative disinfectants for swimming pool disinfection under tropical conditions.

    PubMed

    Yang, Linyan; Schmalz, Christina; Zhou, Jin; Zwiener, Christian; Chang, Victor W-C; Ge, Liya; Wan, Man Pun

    2016-09-15

    Sodium hypochlorite (NaClO) is the most commonly used disinfectant in pool treatment system. Outdoor pools usually suffer from the strong sunlight irradiation which degrades the free chlorine rapidly. In addition, more pools start to adopt the recirculation of swimming pool water, which intensifies the disinfection by-product (DBP) accumulation issue. Given these potential drawbacks of using NaClO in the tropical environment, two alternative organic-based disinfectants, trichloroisocyanuric acid (TCCA, C3Cl3N3O3) and bromochlorodimethylhydantoin (BCDMH, C5H6BrClN2O2), were investigated and compared to NaClO in terms of their self-degradation and the formation of DBPs, including trihalomethanes (THMs) and haloacetic acids (HAAs), under simulated tropical climate conditions. The result reveals that halogen stabilizer, TCCA, had the advantages of slower free chlorine degradation and lower DBP concentration compared to NaClO, which makes it a good alternative disinfectant. BCDMH was not recommended mainly due to the highly reactive disinfecting ingredient, hypobromous acid (HBrO), which fails to sustain the continuous disinfection requirement. Total disinfectant dosage was the main factor that affects residual chlorine/bromine and THM/HAA formation regardless of different disinfectant dosing methods, e.g. shock dosing (one-time spiking) in the beginning, and continuous dosing during the whole experimental period. Two-stage second-order-kinetic-based models demonstrate a good correlation between the measured and predicted data for chlorine decay (R(2) ≥ 0.95), THM (R(2) ≥ 0.99) and HAA (R(2) ≥ 0.83) formation. Higher temperature was found to enhance the DBP formation due to the temperature dependence of reaction rates. Thus, temperature control of pools, especially for those preferring higher temperatures (e.g. hydrotherapy and spa), should take both bather comfort and DBP formation potential into consideration. It is also observed that chlorine competition

  11. ALTERNATIVES FOR REDUCING INSECTICIDES ON COTTON AND CORN: ECONOMIC AND ENVIRONMENTAL IMPACT - SUPPLEMENT 2: PROCEDURES USED IN SETTING UP THE AGRICULTURAL PRODUCTION MODEL

    EPA Science Inventory

    The procedures used in setting up the agricultural production model used in a study of alternatives for reducing insecticides on cotton and corn are described. The major analytical tool used is a spatial equilibrium model of U.S. agriculture. This is a linear programming model th...

  12. Age-Sensitive Effects of Enduring Work with Alternating Cognitive and Physical Load. A Study Applying Mobile EEG in a Real Life Working Scenario

    PubMed Central

    Wascher, Edmund; Heppner, Holger; Kobald, Sven O.; Arnau, Stefan; Getzmann, Stephan; Möckel, Tina

    2016-01-01

    Ergonomic assessment of a workplace requires the evaluation of physical as well as cognitive aspects of a particular working situation. In particular the latter is hardly possible without interfering in the natural setting. Mobile acquisition of neurophysiological measures (such as parameters of the EEG) may close this gap. At a simulated workplace we tracked older and younger participants with mobile EEG during a 4–5 h work shift. They had to perform either a monotonous cognitive task, a self-paced cognitive task or a self-paced physical task in a predefined order. Self assessment, behavioral performance and spectral measures of the EEG (before most alpha power) indicated that younger participants suffered from monotony. Older adults, on the other hand, were overall impaired by inefficient information processing. This was visible in EEG variations time-locked to eye blinks (blink-related synchronizations), a new measure to investigate cognitive processing in real life environments. Thus, we were able to distinguish between active and passive task-related aspects of mental fatigue without impinging on the natural working situation. PMID:26793091

  13. Productive Resources in Students' Ideas about Energy: An Alternative Analysis of Watts' Original Interview Transcripts

    ERIC Educational Resources Information Center

    Harrer, Benedikt W.; Flood, Virginia J.; Wittmann, Michael C.

    2013-01-01

    For over 30 years, researchers have investigated students' ideas about energy with the intent of reforming instructional practice. In this pursuit, Watts contributed an influential study with his 1983 paper "Some alternative views of energy" ["Phys. Educ." 18, 213 (1983)]. Watts' "alternative frameworks"…

  14. 21 CFR 201.26 - Exceptions or alternatives to labeling requirements for human drug products held by the Strategic...

    Code of Federal Regulations, 2012 CFR

    2012-04-01

    ... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL... grant an exception or alternative to any provision listed in paragraph (f) of this section and not... a Strategic National Stockpile official, a written request for an exception or alternative...

  15. 21 CFR 201.26 - Exceptions or alternatives to labeling requirements for human drug products held by the Strategic...

    Code of Federal Regulations, 2013 CFR

    2013-04-01

    ... Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS: GENERAL... grant an exception or alternative to any provision listed in paragraph (f) of this section and not... a Strategic National Stockpile official, a written request for an exception or alternative...

  16. Development of real-time line-scan hyperspectral imaging system for online agricultural and food product inspection

    Technology Transfer Automated Retrieval System (TEKTRAN)

    This paper reports a recent development of a line-scan hyperspectral imaging system for real-time multispectral imaging applications in agricultural and food industries. The hyperspectral imaging system consisted of a spectrograph, an EMCCD camera, and application software. The real-time multispectr...

  17. An Internal Reference Control Duplex Real-Time Polymerase Chain Reaction Assay for Detecting Bacterial Contamination in Blood Products.

    PubMed

    Zhang, Jin-Ju; Tian, Jing-Jing; Wei, Shuang-Shi; Duan, Sheng-Bao; Wang, Hong-Mei; Chen, Ye-Zhou; Ding, Shao-Hua; Zhang, Chun; Meng, Qing-Lin; Li, Yong

    2015-01-01

    Real-time polymerase chain reaction (RT-PCR) enables effective and sensitive screening for infectious risk in the field of blood safety. However, when using RT-PCR to detect bacterial contamination, several intractable points must be considered, one of which is the lack of appropriate quality control. In this study, we developed a simplified RT-PCR assay in which the same primer set and two distinct probes were used to detect both, an internal reference control and the target in a reaction. The copy number of the internal reference control represents the positive detection limit of the assay; therefore, when the threshold-cycle value of the target is less than or equal to that of the internal reference control, the result obtained for the target can be considered to be a true positive. When human gDNA was spiked with Escherichia coli gDNA and the detection limit for the internal reference control was set to five copies, the measured detection limit for E. coli gDNA was two copies. The internal reference control duplex RT-PCR assay showed high efficiency (0.91-1.02), high linearity (R2 > 0.99), and good reproducibility in intra- and inter-assay comparisons. Lastly, when human platelet-rich plasma samples were spiked with E. coli or other bacterial species, all species were detected efficiently, and the results of a two-sample pooled t test showed that the limit of detection for E. coli was 1 cfu/mL. Here, we present a synthetic internal reference control molecule and a new statistical method for improving the reliability of RT-PCR assays when screening for bacterial contamination in blood products. PMID:26230627

  18. LEMming: A Linear Error Model to Normalize Parallel Quantitative Real-Time PCR (qPCR) Data as an Alternative to Reference Gene Based Methods

    PubMed Central

    Feuer, Ronny; Vlaic, Sebastian; Arlt, Janine; Sawodny, Oliver; Dahmen, Uta; Zanger, Ulrich M.; Thomas, Maria

    2015-01-01

    Background Gene expression analysis is an essential part of biological and medical investigations. Quantitative real-time PCR (qPCR) is characterized with excellent sensitivity, dynamic range, reproducibility and is still regarded to be the gold standard for quantifying transcripts abundance. Parallelization of qPCR such as by microfluidic Taqman Fluidigm Biomark Platform enables evaluation of multiple transcripts in samples treated under various conditions. Despite advanced technologies, correct evaluation of the measurements remains challenging. Most widely used methods for evaluating or calculating gene expression data include geNorm and ΔΔCt, respectively. They rely on one or several stable reference genes (RGs) for normalization, thus potentially causing biased results. We therefore applied multivariable regression with a tailored error model to overcome the necessity of stable RGs. Results We developed a RG independent data normalization approach based on a tailored linear error model for parallel qPCR data, called LEMming. It uses the assumption that the mean Ct values within samples of similarly treated groups are equal. Performance of LEMming was evaluated in three data sets with different stability patterns of RGs and compared to the results of geNorm normalization. Data set 1 showed that both methods gave similar results if stable RGs are available. Data set 2 included RGs which are stable according to geNorm criteria, but became differentially expressed in normalized data evaluated by a t-test. geNorm-normalized data showed an effect of a shifted mean per gene per condition whereas LEMming-normalized data did not. Comparing the decrease of standard deviation from raw data to geNorm and to LEMming, the latter was superior. In data set 3 according to geNorm calculated average expression stability and pairwise variation, stable RGs were available, but t-tests of raw data contradicted this. Normalization with RGs resulted in distorted data contradicting

  19. Understanding of real alternative redox partner of Streptomyces peucetius DoxA: Prediction and validation using in silico and in vitro analyses.

    PubMed

    Rimal, Hemraj; Lee, Seung-Won; Lee, Joo-Ho; Oh, Tae-Jin

    2015-11-01

    Streptomyces peucetius ATCC27952 contains the cytochrome P450 monoxygenase DoxA that is responsible for the hydroxylation of daunorubicin into doxorubicin. Although S. peucetius ATCC27952 contains several potential redox partners, the most suitable endogenous electron-transport system is still unclear; therefore, we conducted a study of potential redox partners using Accelrys Discovery Studio 3.5. Recombinant DoxA along with its redox partners from S. peucetius FDX1, FDR2, and FDX3, and the putidaredoxin and putidaredoxin reductase from Pseudomonas putida that are essential equivalents of the class I type of bacterial electron-transport system were over-expressed and purified. The successful development of an efficient redox system was achieved by an in vitro enzymatic catalysis reaction with DoxA. The optimal pH for the activation of the heme was 7.6 and the optimal temperature was 30 °C. Our findings suggest a two-fold increase of DoxA activity via the NADH → FDR2 → FDX1 → DoxA pathway for the hydroxylation of the daunorubicin, and indicate that the usage of a native redox partner may increase daunorubicin-derived doxorubicin production due to the inclusion of DoxA. PMID:26334717

  20. Alternative security

    SciTech Connect

    Weston, B.H. )

    1990-01-01

    This book contains the following chapters: The Military and Alternative Security: New Missions for Stable Conventional Security; Technology and Alternative Security: A Cherished Myth Expires; Law and Alternative Security: Toward a Just World Peace; Politics and Alternative Security: Toward a More Democratic, Therefore More Peaceful, World; Economics and Alternative Security: Toward a Peacekeeping International Economy; Psychology and Alternative Security: Needs, Perceptions, and Misperceptions; Religion and Alternative Security: A Prophetic Vision; and Toward Post-Nuclear Global Security: An Overview.

  1. Real Earthquakes, Real Learning

    ERIC Educational Resources Information Center

    Schomburg, Aaron

    2003-01-01

    One teacher took her class on a year long earthquake expedition. The goal was to monitor the occurrences of real earthquakes during the year and mark their locations with push pins on a wall-sized world map in the hallway outside the science room. The purpose of the project was to create a detailed picture of the earthquakes that occurred…

  2. Evaluation of Alternatives to Carbamate and Organophosphate Insecticides Against Thrips and Tomato Spotted Wilt Virus in Peanut Production.

    PubMed

    Marasigan, K; Toews, M; Kemerait, R; Abney, M R; Culbreath, A; Srinivasan, R

    2016-04-01

    Thrips are important pests of peanut. They cause severe feeding injuries on peanut foliage in the early season. They also transmit Tomato spotted wilt virus (TSWV), which causes spotted wilt disease. At-plant insecticides and cultivars that exhibit field resistance to TSWV are often used to manage thrips and spotted wilt disease. Historically, peanut growers used the broad-spectrum insecticides aldicarb (IRAC class 1A; Temik) and phorate (IRAC class 1B; Thimet) for managing thrips and thereby reducing TSWV transmission. Aldicarb has not been produced since 2011 and its usage in peanut will be legally phased out in 2018; therefore, identification of alternative chemistries is critical for thrips and spotted wilt management. Here, eight alternative insecticides, with known thrips activity, were evaluated in field trials conducted from 2011 through 2013. In addition, different application methods of alternatives were also evaluated. Imidacloprid (Admire Pro), thiamethoxam (Actara), spinetoram (Radiant), and cyantraniliprole (Exirel) were as effective as aldicarb and phorate in suppressing thrips, but none of the insecticides significantly suppressed spotted wilt incidence. Nevertheless, greenhouse assays demonstrated that the same alternative insecticides were effective in suppressing thrips feeding and reducing TSWV transmission. Spotted wilt incidence in the greenhouse was more severe (∼80%) than in the field (5–25%). In general, field resistance to TSWV in cultivars only marginally influenced spotted wilt incidence. Results suggest that effective management of thrips using alternative insecticides and subsequent feeding reduction could improve yields under low to moderate virus pressure. PMID:26637534

  3. Development of Productive Skills. Report of a Sub-Regional APEID Workshop on Non-Formal and Alternative Structures in Education with Emphasis on Development of Productive Skills (Philippines, May 28-June 16, 1979).

    ERIC Educational Resources Information Center

    United Nations Educational, Scientific, and Cultural Organization, Bangkok (Thailand). Regional Office for Education in Asia and Oceania.

    Delegates from six countries (Bangladesh, Korea, India, Indonesia, Thailand, and the Philippines) met in the Philippines in May-June 1979 at a UNESCO-sponsored conference to discuss development of productive skills through nonformal and alternative structures in education. The main part of the meeting was a series of reports of the nonformal…

  4. Teaching Educational Assistants to Facilitate the Multisymbol Message Productions of Young Students Who Require Augmentative and Alternative Communication

    ERIC Educational Resources Information Center

    Binger, Cathy; Kent-Walsh, Jennifer; Ewing, Cai; Taylor, Stacy

    2010-01-01

    Purpose: Many classroom educational assistants (EAs) have a significant amount of responsibility in carrying out educational plans for children who use augmentative and alternative communication (AAC), but they receive little instruction on how to do so (Kent-Walsh & Light, 2003). This study investigates the impact of using a communication partner…

  5. Educational Alternatives in Food Production, Knowledge and Consumption: The Public Pedagogies of "Growing Power" and "Tsyunhehkw"[superscript caret

    ERIC Educational Resources Information Center

    Walter, Pierre

    2012-01-01

    This paper examines how two sites of adult learning in the food movement create educational alternatives to the dominant U.S. food system. It further examines how these pedagogies challenge racialised, classed and gendered ideologies and practices in their aims, curricular content, and publically documented educational processes. The first case is…

  6. A clean start to productive orchards and vineyards: recent research on methyl bromide alternatives for perennial crop nurseries

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Methyl bromide (MB) is an important pest management tool in open field perennial crop nurseries in California for control of many soil borne pests including plant parasitic nematodes, pathogens, and weeds. Because MB is being phased out under the provisions of the Montreal Protocol, alternatives are...

  7. Monitoring Regional Forest Disturbances across the US with Near Real Time MODIS NDVI Products included in the ForWarn Forest Threat Early Warning System

    NASA Technical Reports Server (NTRS)

    Spruce, Joseph; Hargrove, William W.; Gasser, Gerald; Norman, Steve

    2013-01-01

    U.S. forests occupy approx.1/3 of total land area (approx. 304 million ha). Since 2000, a growing number of regionally evident forest disturbances have occurred due to abiotic and biotic agents. Regional forest disturbances can threaten human life and property, bio-diversity and water supplies. Timely regional forest disturbance monitoring products are needed to aid forest health management work. Near Real Time (NRT) twice daily MODIS NDVI data provide a means to monitor U.S. regional forest disturbances every 8 days. Since 2010, these NRT forest change products have been produced and posted on the US Forest Service ForWarn Early Warning System for Forest Threats.

  8. Aerosols and contact insecticides as alternatives to methyl bromide in flour mills, food production facilities, and food warehouses

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The fumigant methyl bromide (MB) is being phased out of production and usage to control stored product insects in flour and rice mills, as well as feed and food production plants, in the United States (US) and other developed countries throughout the world. A phase-out schedule has also been establi...

  9. Development of real-time line-scan hyperspectral imaging system for online agricultural and food product inspection

    NASA Astrophysics Data System (ADS)

    Yoon, Seung Chul; Park, Bosoon; Lawrence, Kurt C.; Windham, William R.; Heitschmidt, Gerald W.

    2010-04-01

    This paper reports a recent development of a line-scan hyperspectral imaging system for real-time multispectral imaging applications in agricultural and food industries. The hyperspectral imaging system consisted of a spectrograph, an EMCCD camera, and application software. The real-time multispectral imaging with the developed system was possible due to (1) data binning, especially a unique feature of the EMCCD sensor allowing the access to non-contiguous multispectral bands, (2) an image processing algorithm designed for real-time multispectral imaging, and (3) the design and implementation of the real-time application software. The imaging system was developed as a poultry inspection instrument determining the presence of surface feces on poultry carcasses moving at commercial poultry processing line speeds up to 180 birds per minute. The imaging system can be easily modifiable to solve other real-time inspection/sorting problems. Three wavelengths at 517 nm, 565 nm and 802 nm were selected for real-time fecal detection imaging. The fecal detection algorithm was based on dual band ratios of 565nm/517nm and 802nm/517nm followed by thresholding. The software architecture was based on a ping pong memory and a circular buffer for the multitasking of image grabbing and processing. The software was written in Microsoft Visual C++. An image-based internal triggering (i.e. polling) algorithm was developed to determine the start and end positions of birds. Twelve chickens were used for testing the imaging system at two different speeds (140 birds per minute and 180 bird per minute) in a pilot-scale processing line. Four types of fecal materials (duodenum, ceca, colon and ingesta) were used for the evaluation of the detection algorithm. The software grabbed and processed multispectral images of the dimension 118 (line scans) x 512 (height) x 3 (bands) pixels obtained from chicken carcasses moving at the speed up to 180 birds per minute (a frame rate 286 Hz). Intensity

  10. Depleted uranium management alternatives

    SciTech Connect

    Hertzler, T.J.; Nishimoto, D.D.

    1994-08-01

    This report evaluates two management alternatives for Department of Energy depleted uranium: continued storage as uranium hexafluoride, and conversion to uranium metal and fabrication to shielding for spent nuclear fuel containers. The results will be used to compare the costs with other alternatives, such as disposal. Cost estimates for the continued storage alternative are based on a life-cycle of 27 years through the year 2020. Cost estimates for the recycle alternative are based on existing conversion process costs and Capital costs for fabricating the containers. Additionally, the recycle alternative accounts for costs associated with intermediate product resale and secondary waste disposal for materials generated during the conversion process.

  11. Sensitive and specific detection of pine nut (Pinus spp.) by real-time PCR in complex food products.

    PubMed

    Garino, Cristiano; De Paolis, Angelo; Coïsson, Jean Daniel; Bianchi, Daniela Manila; Decastelli, Lucia; Arlorio, Marco

    2016-03-01

    Pine nuts are a known source of food allergens and several cases of adverse immunological reaction after ingestion have been reported. To protect allergic consumers, methods to unequivocally detect the presence of pine nuts in complex matrices must be developed. A Taqman-based real time PCR method for the detection of Pinus spp. was set up. A homemade pesto spiked at known concentration of pine nut powder was used as model food. Moreover, DNA was purified from commercial foods declaring or not the presence of pine nuts. The method displayed a very high efficiency and specificity for the genus Pinus. The intrinsic LOD was 1pg of DNA, while the practical LOD evaluated on model foods was 0.1ppm of pine nuts powder, the lowest ever registered for the detection of food allergens via real-time PCR. Finally, the declared presence/absence of pine nut in commercial foods was confirmed. PMID:26471643

  12. Comparative studies of a real-time PCR method and three enzyme-linked immunosorbent assays for the detection of central nervous system tissues in meat products.

    PubMed

    Schönenbrücher, Holger; Göbel, Katrin Annette; Abdulmawjood, Amir; Richt, Jürgen A; Bülte, Michael

    2008-10-01

    The removal of certain central nervous system (CNS) tissues (part of the bovine spongiform encephalopathy risk material) from the food chain is one of the highest priority tasks associated with avoiding contamination of the human food chain with the agent of bovine spongiform encephalopathy. A recently developed real-time PCR assay and three commercially available enzyme-linked immunosorbent assays (ELISAs) for the detection of CNS tissues in minced meat and three types of heat-treated sausages were evaluated. Bovine brain was used for spiking of internal reference material, and its detectability was examined during storage times of 12 months (for frozen minced meat and liver sausage) and 24 months (for sausages treated with medium and high heat). The real-time PCR method and both ELISA kits detected 0.1% CNS tissue in frozen minced meat and 0.1 or 1% CNS tissue in heat-treated meat products. The detectability of the amplified mRNA target region with the PCR assay was similar to the detectability of antigen by the ELISAs. Because the real-time PCR method also can be used to distinguish cattle, ovine, and caprine CNS tissues from porcine CNS tissues, it seems to be suitable as a routine diagnostic test for the sensitive and specific detection of CNS tissues in meat and meat products. PMID:18939753

  13. Multi-GNSS real-time precise orbit/clock/UPD products and precise positioning service at GFZ

    NASA Astrophysics Data System (ADS)

    Li, Xingxing; Ge, Maorong; Liu, Yang; Fritsche, Mathias; Wickert, Jens; Schuh, Harald

    2016-04-01

    The rapid development of multi-constellation GNSSs (Global Navigation Satellite Systems, e.g., BeiDou, Galileo, GLONASS, GPS) and the IGS (International GNSS Service) Multi-GNSS Experiment (MGEX) bring great opportunities and challenges for real-time precise positioning service. In this contribution, we present a GPS+GLONASS+BeiDou+Galileo four-system model to fully exploit the observations of all these four navigation satellite systems for real-time precise orbit determination, clock estimation and positioning. A rigorous multi-GNSS analysis is performed to achieve the best possible consistency by processing the observations from different GNSS together in one common parameter estimation procedure. Meanwhile, an efficient multi-GNSS real-time precise positioning service system is designed and demonstrated by using the Multi-GNSS Experiment (MGEX) and International GNSS Service (IGS) data streams including stations all over the world. The addition of the BeiDou, Galileo and GLONASS systems to the standard GPS-only processing, reduces the convergence time almost by 70%, while the positioning accuracy is improved by about 25%. Some outliers in the GPS-only solutions vanish when multi-GNSS observations are processed simultaneous. The availability and reliability of GPS precise positioning decrease dramatically as the elevation cutoff increases. However, the accuracy of multi-GNSS precise point positioning (PPP) is hardly decreased and few centimeters are still achievable in the horizontal components even with 40° elevation cutoff.

  14. 21 CFR 201.26 - Exceptions or alternatives to labeling requirements for human drug products held by the Strategic...

    Code of Federal Regulations, 2010 CFR

    2010-04-01

    ...) Identify the specified lots, batches, or other units of the human drug product that would be subject to the... requirements for human drug products held by the Strategic National Stockpile. 201.26 Section 201.26 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS:...

  15. 21 CFR 809.11 - Exceptions or alternatives to labeling requirements for in vitro diagnostic products for human...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ... requirements for in vitro diagnostic products for human use held by the Strategic National Stockpile. 809.11 Section 809.11 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) MEDICAL DEVICES IN VITRO DIAGNOSTIC PRODUCTS FOR HUMAN USE Labeling § 809.11 Exceptions...

  16. 21 CFR 201.26 - Exceptions or alternatives to labeling requirements for human drug products held by the Strategic...

    Code of Federal Regulations, 2011 CFR

    2011-04-01

    ...) Identify the specified lots, batches, or other units of the human drug product that would be subject to the... requirements for human drug products held by the Strategic National Stockpile. 201.26 Section 201.26 Food and Drugs FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES (CONTINUED) DRUGS:...

  17. Real and hypothetical rewards.

    PubMed

    Locey, Matthew L; Jones, Bryan A; Rachlin, Howard

    2011-08-01

    Laboratory studies of choice and decision making among real monetary rewards typically use smaller real rewards than those common in real life. When laboratory rewards are large, they are almost always hypothetical. In applying laboratory results meaningfully to real-life situations, it is important to know the extent to which choices among hypothetical rewards correspond to choices among real rewards and whether variation of the magnitude of hypothetical rewards affects behavior in meaningful ways. The present study compared real and hypothetical monetary rewards in two experiments. In Experiment 1, participants played a temporal discounting game that incorporates the logic of a repeated prisoner's-dilemma (PD) type game versus tit-for-tat; choice of one alternative ("defection" in PD terminology) resulted in a small-immediate reward; choice of the other alternative ("cooperation" in PD terminology) resulted in a larger reward delayed until the following trial. The larger-delayed reward was greater for half of the groups than for the other half. Rewards also differed in type across groups: multiples of real nickels, hypothetical nickels or hypothetical hundred-dollar bills. All groups significantly increased choice of the larger delayed reward over the 40 trials of the experiment. Over the last 10 trials, cooperation was significantly higher when the difference between larger and smaller hypothetical rewards was greater. Reward type (real or hypothetical) made no significant difference in cooperation. In Experiment 2, real and hypothetical rewards were compared in social discounting - the decrease in value to the giver of a reward as social distance increases to the receiver of the reward. Social discount rates were well described by a hyperbolic function. Discounting rates for real and hypothetical rewards did not significantly differ. These results add to the evidence that results of experiments with hypothetical rewards validly apply in everyday life. PMID

  18. Net energy production and emissions mitigation of domestic wastewater treatment system: a comparison of different biogas-sludge use alternatives.

    PubMed

    Chen, Shaoqing; Chen, Bin

    2013-09-01

    Wastewater treatment systems are increasingly designed for the recovery of valuable chemicals and energy in addition to waste stream disposal. Herein, the life-cycle energy production and emissions mitigation of a typical domestic wastewater treatment system were assessed, in which different combinations of biogas use and sludge processing lines for industrial or household applications were considered. The results suggested that the reuse of biogas and sludge was so important in the system's overall energy balance and environmental performance that it may offset the cost in the plant's installation and operation. Combined heat and power and household utilization were two prior options for net energy production, provided an ideal power conversion efficiency and biogas production. The joint application of household biogas use and sludge nutrient processing achieved both high net energy production and significant environmental remediation across all impact categories, representing the optimal tradeoff for domestic wastewater treatment. PMID:23880131

  19. High-throughput process development of an alternative platform for the production of virus-like particles in Escherichia coli.

    PubMed

    Ladd Effio, Christopher; Baumann, Pascal; Weigel, Claudia; Vormittag, Philipp; Middelberg, Anton; Hubbuch, Jürgen

    2016-02-10

    The production of safe vaccines against untreatable or new diseases has pushed the research in the field of virus-like particles (VLPs). Currently, a large number of commercial VLP-based human vaccines and vaccine candidates are available or under development. A promising VLP production route is the controlled in vitro assembly of virus proteins into capsids. In the study reported here, a high-throughput screening (HTS) procedure was implemented for the upstream process development of a VLP platform in bacterial cell systems. Miniaturized cultivations were carried out in 48-well format in the BioLector system (m2p-Labs, Germany) using an Escherichia coli strain with a tac promoter producing the murine polyomavirus capsid protein (VP1). The screening procedure incorporated micro-scale cultivations, HTS cell disruption by sonication and HTS-compatible analytics by capillary gel electrophoresis. Cultivation temperatures, shaking speeds, induction and medium conditions were varied to optimize the product expression in E. coli. The most efficient system was selected based on an evaluation of soluble and insoluble product concentrations as well as on the percentage of product in the total soluble protein fraction. The optimized system was scaled up to cultivation 2.5L shaker flask scale and purified using an anion exchange chromatography membrane adsorber, followed by a size exclusion chromatography polishing procedure. For proof of concept, purified VP1 capsomeres were assembled under defined buffer conditions into empty capsids and characterized using transmission electron microscopy (TEM). The presented HTS procedure allowed for a fast development of an efficient production process of VLPs in E. coli. Under optimized cultivation conditions, the VP1 product totalled up to 43% of the total soluble protein fraction, yielding 1.63 mg VP1 per mL of applied cultivation medium. The developed production process strongly promotes the murine polyoma-VLP platform, moving towards

  20. Alternate Alternates: A Medley of Alternate Assessments.

    ERIC Educational Resources Information Center

    Burdette, Paula J.; Olsen, Ken

    This paper highlights eight states that have implemented alternate assessments for children with disabilities who cannot participate in their state and district-wide assessment programs. The alternate assessment systems in Delaware, Florida, Georgia, Indiana, Minnesota, North Dakota, Utah, and West Virginia are briefly described, along with their…

  1. Mandarin-Speaking Three-Year-Olds' Demonstration of Productive Knowledge of Syntax: Evidence from Syntactic Productivity and Structural Priming with the SVO-"ba" Alternation

    ERIC Educational Resources Information Center

    Hsu, Dong Bo

    2014-01-01

    Two studies investigated syntactic productivity in three-year-old Mandarin speakers' use of verbs in the SVO and S"ba"OV constructions. In Study 1, children were taught novel verbs in one construction and assessed for their production in the other construction. Children produced verbs taught in the "ba" constructions in…

  2. Growth performance of fast growing broilers reared under different types of production systems with outdoor access: Implications for organic and alternative production systems

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Outdoor access is an important part of organic and free range poultry production, yet there is limited information on the impact of various housing and production systems on growth performance and colonization of foodborne pathogens. Therefore, the primary purpose of this study was to evaluate the ...

  3. Promotion of atomic hydrogen recombination as an alternative to electron trapping for the role of metals in the photocatalytic production of H2

    PubMed Central

    Joo, Ji Bong; Dillon, Robert; Lee, Ilkeun; Yin, Yadong; Bardeen, Christopher J.; Zaera, Francisco

    2014-01-01

    The production of hydrogen from water with semiconductor photocatalysts can be promoted by adding small amounts of metals to their surfaces. The resulting enhancement in photocatalytic activity is commonly attributed to a fast transfer of the excited electrons generated by photon absorption from the semiconductor to the metal, a step that prevents deexcitation back to the ground electronic state. Here we provide experimental evidence that suggests an alternative pathway that does not involve electron transfer to the metal but requires it to act as a catalyst for the recombination of the hydrogen atoms made via the reduction of protons on the surface of the semiconductor instead. PMID:24843154

  4. High-level production of recombinant trypsin in transgenic rice cell culture through utilization of an alternative carbon source and recycling system.

    PubMed

    Kim, Nan-Sun; Yu, Hwa-Young; Chung, Nguyen-Duc; Kwon, Tae-Ho; Yang, Moon-Sik

    2014-09-01

    Productivity of recombinant bovine trypsin using a rice amylase 3D promoter has been studied in transgenic rice suspension culture. Alternative carbon sources were added to rice cell suspension cultures in order to improve the production of recombinant bovine trypsin. It was demonstrated that addition of alternative carbon sources such as succinic acid, fumaric acid and malic acid in the culture medium could increase the productivity of recombinant bovine trypsin 3.8-4.3-fold compared to those in the control medium without carbon sources. The highest accumulated trypsin reached 68.2 mg/L on day 5 in the culture medium with 40 mM fumaric acid. The feasibility of repeated use of the cells for recombinant trypsin production was tested in transgenic rice cell suspension culture with the culture medium containing the combination of variable sucrose concentration and 40 mM fumaric acid. Among the used combinations, the combination of 1% sucrose and 40 mM fumaric acid resulted in a yield of up to 53 mg/L five days after incubation. It also increased 31% (W/W) of dry cell weight and improved 43% of cell viability compared to that in control medium without sucrose. Based on these data, recycling of the trypsin production process with repeated 1% sucrose and 40 mM fumaric acid supplying-harvesting cycles was developed in flask scale culture. Recombinant bovine trypsin could be stably produced with a yield of up to 53-39 mg/L per cycle during five recycling cycles. PMID:25039055

  5. Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1).

    PubMed

    Van Eynde, A; Pérez-Callejón, E; Schoenmakers, E; Jacquemin, M; Stalmans, W; Bollen, M

    1999-04-01

    Nuclear inhibitor of protein phosphatase-1 (NIPP-1) is one of two major regulatory subunits of protein phosphatase-1 in mammalian nuclei. We report here the cloning and structural characterization of the human NIPP-1 genes, designated PPP1R8P and PPP1R8 in human gene nomenclature. PPP1R8P (1.2 kb) is a processed pseudogene and was localized by in situ hybridization to chromosome 1p33-32. PPP1R8 is an authentic NIPP-1 gene and was localized to chromosome 1p35. PPP1R8 (25.2 kb) is composed of seven exons and encodes four different transcripts, as determined from cDNA library screening, reverse transcriptase-PCR (RT-PCR) and/or EST (expressed sequence tag) database search analysis. NIPP-1alpha mRNA represents the major transcript in human tissues and various cell lines, and encodes a polypeptide of 351 residues that only differs from the previously cloned calf thymus NIPP-1 by a single residue. The other transcripts, termed NIPP-1beta, gamma and delta, are generated by alternative 5'-splice site usage, by exon skipping and/or by alternative polyadenylation. The NIPP-1beta/delta and NIPP-1gamma mRNAs are expected to encode fragments of NIPP-1alpha that differ from the latter by the absence of the first 142 and 224 residues, respectively. NIPP-1gamma corresponds to 'activator of RNA decay-1' (Ard-1) which, unlike NIPP-1alpha, displays in vitro and endoribonuclease activity and lacks an RVXF consensus motif for interaction with protein phosphatase-1. While the NIPP-1alpha/beta/delta-transcripts were found to be present in various human tissues, the NIPP-1gamma transcript could only be detected in human transformed B-lymphocytes. PMID:10103062

  6. NYC condom use and satisfaction and demand for alternative condom products in New York City sexually transmitted disease clinics.

    PubMed

    Burke, Ryan C; Wilson, Juliet; Kowalski, Alexis; Murrill, Christopher; Cutler, Blayne; Sweeney, Monica; Begier, Elizabeth M

    2011-08-01

    In 2007, via a high-profile media campaign, the New York City Department of Health and Mental Hygiene (NYC DOHMH) introduced the "NYC Condom," the first specially packaged condom unique to a municipality. We conducted a survey to measure NYC Condom awareness of and experience with NYC Condoms and demand for alternative male condoms to be distributed by the DOHMH. Trained interviewers administered short, in-person surveys at five DOHMH-operated sexually transmitted disease (STD) clinics in Spring 2008. We systematically sampled eligible patients: NYC residents aged ≥18 years waiting to see a physician. We approached 539; 532 agreed to be screened (98.7% response rate); 462 completed the survey and provided NYC zip codes. Most respondents were male (56%), non-Hispanic black (64%), aged 18-24 years (43%) or 25-44 years (45%), employed (65%), and had a high school degree/general equivalency diploma or less (53%). Of those surveyed, 86% were aware of the NYC Condom, and 81% of those who obtained the condoms used them. NYC Condom users were more likely to have four or more sexual partners in the past 12 months (adjusted odds ratio [AOR] = 2.0, 95% confidence interval [CI] = 1.0-3.8), use condoms frequently (AOR = 2.1, 95% CI = 1.3-3.6), and name an alternative condom for distribution (AOR = 2.2, 95% CI = 1.3-3.9). The most frequently requested condom types respondents wanted DOHMH to provide were larger size (28%), ultra thin/extra sensitive (21%), and extra strength (16%). We found high rates of NYC Condom use. NYC Condom users reported more sexual partners than others, suggesting the condom initiative successfully reached higher-risk persons within the STD clinic population. Study results document the condom social marketing campaign's success. PMID:21792691

  7. Alternative Therapies

    MedlinePlus

    ... Late Effects of Poliomyelitis for Physicians and Survivors © Alternative Therapies Alternative therapies, also called complementary, can support ... of motion, pain, and fatigue are often reported. Energy work includes acupuncture and acupressure, traditional Chinese medicine ...

  8. Alternative disinfectant water treatments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Alternative disinfestant water treatments are disinfestants not as commonly used by the horticultural industry. Chlorine products that produce hypochlorous acid are the main disinfestants used for treating irrigation water. Chlorine dioxide will be the primary disinfestant discussed as an alternativ...

  9. Cancer Alternative Therapies

    MedlinePlus

    ... be thinking about is complementary and alternative medicine (CAM). CAM is the term for medical products and practices ... are not part of standard care. Examples of CAM therapies are acupuncture, chiropractic, and herbal medicines. People ...

  10. The Mississippi University Research Consortium for the Utilization of Biomass: Production of Alternative Fuels from Waste Biomass Initiative

    SciTech Connect

    Drs. Mark E. Zapp; Todd French; Lewis Brown; Clifford George; Rafael Hernandez; Marvin Salin; Drs. Huey-Min Hwang, Ken Lee, Yi Zhang; Maria Begonia; Drs. Clint Williford; Al Mikell; Drs. Robert Moore; Roger Hester .

    2009-03-31

    The Mississippi Consortium for the Utilization of Biomass was formed via funding from the US Department of Energy's EPSCoR Program, which is administered by the Office of Basic Science. Funding was approved in July of 1999 and received by participating Mississippi institutions by 2000. The project was funded via two 3-year phases of operation (the second phase was awarded based on the high merits observed from the first 3-year phase), with funding ending in 2007. The mission of the Consortium was to promote the utilization of biomass, both cultured and waste derived, for the production of commodity and specialty chemicals. These scientific efforts, although generally basic in nature, are key to the development of future industries within the Southeastern United States. In this proposal, the majority of the efforts performed under the DOE EPSCoR funding were focused primarily toward the production of ethanol from lignocellulosic feedstocks and biogas from waste products. However, some of the individual projects within this program investigated the production of other products from biomass feeds (i.e. acetic acid and biogas) along with materials to facilitate the more efficient production of chemicals from biomass. Mississippi is a leading state in terms of raw biomass production. Its top industries are timber, poultry production, and row crop agriculture. However, for all of its vast amounts of biomass produced on an annual basis, only a small percentage of the biomass is actually industrially produced into products, with the bulk of the biomass being wasted. This situation is actually quite representative of many Southeastern US states. The research and development efforts performed attempted to further develop promising chemical production techniques that use Mississippi biomass feedstocks. The three processes that were the primary areas of interest for ethanol production were syngas fermentation, acid hydrolysis followed by hydrolyzate fermentation, and enzymatic

  11. Intercropping of oat and field pea in Alaska: an alternative approach to quality forage production and weed control

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Intercropping of legumes with non-legumes is an ancient crop production method used to improve quality and dry matter (DM) yields of forage and grain, and to control weeds. However, there is little information regarding intercropping at high latitudes. The objectives of this field study were to eval...

  12. Nitrous Oxide Emissions in Medium and Coarse Textured Soils from Corn Production Under Conventional and Alternative Fertilizer Treatments

    Technology Transfer Automated Retrieval System (TEKTRAN)

    Nitrogen (N) fertilizers applied in corn production cause soil-to-atmosphere direct emission of nitrous oxide (N2O), a major greenhouse gas. Some of the added N that leaches into groundwater as nitrate (NO3-) can subsequently be transformed to N2O, which represents an indirect source of N2O emission...

  13. 2,3-Butanediol Production by Acetogenic Bacteria, an Alternative Route to Chemical Synthesis, Using Industrial Waste Gas ▿ †

    PubMed Central

    Köpke, Michael; Mihalcea, Christophe; Liew, FungMin; Tizard, Joseph H.; Ali, Mohammed S.; Conolly, Joshua J.; Al-Sinawi, Bakir; Simpson, Séan D.

    2011-01-01

    2,3-Butanediol (23BD) is a high-value chemical usually produced petrochemically but which can also be synthesized by some bacteria. To date, the best microbial 23BD production rates have been observed using pathogenic bacteria in fermentation systems that depend on sugars as the carbon and energy sources for product synthesis. Here we present evidence of 23BD production by three nonpathogenic acetogenic Clostridium species—Clostridium autoethanogenum, C. ljungdahlii, and C. ragsdalei—using carbon monoxide-containing industrial waste gases or syngas as the sole source of carbon and energy. Through an analysis of the C. ljungdahlii genome, the complete pathway from carbon monoxide to 23BD has been proposed. Homologues of the genes involved in this pathway were also confirmed for the other two species investigated. A gene expression study demonstrates a correlation between mRNA accumulation from 23BD biosynthetic genes and the onset of 23BD production, while a broader expression study of Wood-Ljungdahl pathway genes provides a transcription-level view of one of the oldest existing biochemical pathways. PMID:21685168

  14. SYBR green real time-polymerase chain reaction as a rapid and alternative assay for the efficient identification of all existing Escherichia coli biotypes approved directly in wastewater samples.

    PubMed

    Chetta, Massimiliano; Bafunno, Valeria; Grillo, Rosalba; Mele, Antonio; Lo Perfido, Pietro; Notarnicola, Michele; Cellini, Francesco; Cifarelli, Rosa Anna

    2012-07-01

    Escherichia coli has been recognized as the principal indicator of fecal contamination of water. Indeed, E. coli is the only species in the coliform group found in relationship with gastrointestinal tract of human and warm-blooded animals and subsequently excreted in large numbers in the human feces. To obtain a complete picture of water quality and therefore, a better protection of public health, different techniques for water analysis have been proposed. In this article, we describe an alternative method that uses SYBR green real time-polymerase chain reaction (RT-PCR) technology to identify and quantify all E. coli biotypes in a group of wastewater samples collected from a wastewater depurator located in South of Italy. This new RT-PCR protocol is accurate in measuring the concentration of chromosomal E. coli DNA using the amplification of three new specific fragments of the following bacteria genes: CadC, HNS, and Allan whose sequence is specific for E. coli family and conserved in all E. coli subtypes. This method allowed us to detect the presence of all E. coli biotypes directly in wastewater samples and estimated the correspondence between colony forming units and bacterial DNA concentrations. The availability of a rapid and sensitive method may be useful to monitor the persistence of E. coli in water, to evaluate the efficiency of wastewater purification treatments and the possible recycle for agricultural use. Furthermore, the development of a simple and routine method to monitor water quality with RT-PCR analysis can encourage the testing of a higher number of samples. PMID:22730251

  15. Co-registration of speech production datasets from electromagnetic articulography and real-time magnetic resonance imaging

    PubMed Central

    Kim, Jangwon; Lammert, Adam C.; Kumar Ghosh, Prasanta; Narayanan, Shrikanth S.

    2014-01-01

    This paper describes a spatio-temporal registration approach for speech articulation data obtained from electromagnetic articulography (EMA) and real-time Magnetic Resonance Imaging (rtMRI). This is motivated by the potential for combining the complementary advantages of both types of data. The registration method is validated on EMA and rtMRI datasets obtained at different times, but using the same stimuli. The aligned corpus offers the advantages of high temporal resolution (from EMA) and a complete mid-sagittal view (from rtMRI). The co-registration also yields optimum placement of EMA sensors as articulatory landmarks on the magnetic resonance images, thus providing richer spatio-temporal information about articulatory dynamics. PMID:25234914

  16. Towards a Three-Dimensional Near-Real Time Cloud Product for Aviation Safety and Weather Diagnoses

    NASA Technical Reports Server (NTRS)

    Minnis, Patrick; Nguyen, Louis; Palikonda, Rabindra; Spangeberg, Douglas; Nordeen, Michele L.; Yi, Yu-Hong; Ayers, J. Kirk

    2004-01-01

    Satellite data have long been used for determining the extent of cloud cover and for estimating the properties at the cloud tops. The derived properties can also be used to estimate aircraft icing potential to improve the safety of air traffic in the region. Currently, cloud properties and icing potential are derived in near-real time over the United States of America (USA) from the Geostationary Operational Environmental Satellite GOES) imagers at 75 W and 135 W. Traditionally, the results have been given in two dimensions because of the lack of knowledge about the vertical extent of clouds and the occurrence of overlapping clouds. Aircraft fly in a three-dimensional space and require vertical as well as horizontal information about clouds, their intensity, and their potential for icing. To improve the vertical component of the derived cloud and icing parameters, this paper explores various methods and datasets for filling in the three-dimensional space over the USA with cloud water.

  17. Energy balances in the production and end use of alcohols derived from biomass. A fuels-specific comparative analysis of alternate ethanol production cycles

    SciTech Connect

    Not Available

    1980-10-01

    Considerable public interest and debate have been focused on the so-called energy balance issue involved in the conversion of biomass materials into ethanol for fuel use. This report addresses questions of net gains in premium fuels that can be derived from the production and use of ethanol from biomass, and shows that for the US alcohol fuel program, energy balance need not be a concern. Three categories of fuel gain are discussed in the report: (1) Net petroleum gain; (2) Net premium fuel gain (petroleum and natural gas); and (3) Net energy gain (for all fuels). In this study the investment of energy (in the form of premium fuels) in alcohol production includes all investment from cultivating, harvesting, or gathering the feedstock and raw materials, through conversion of the feedstock to alcohol, to the delivery to the end-user. To determine the fuel gains in ethanol production, six cases, encompassing three feedstocks, five process fuels, and three process variations, have been examined. For each case, two end-uses (automotive fuel use and replacement of petrochemical feedstocks) were scrutinized. The end-uses were further divided into three variations in fuel economy and two different routes for production of ethanol from petrochemicals. Energy requirements calculated for the six process cycles accounted for fuels used directly and indirectly in all stages of alcohol production, from agriculture through distribution of product to the end-user. Energy credits were computed for byproducts according to the most appropriate current use.

  18. Comparative evaluation of pumice stone as an alternative immobilization material for 1,3-propanediol production from waste glycerol by immobilized Klebsiella pneumoniae.

    PubMed

    Gonen, Cagdas; Gungormusler, Mine; Azbar, Nuri

    2012-12-01

    In this study, pumice stone (PS), which is a vastly available material in Turkey, was evaluated as an alternative immobilization material in comparison to other commercially available immobilization materials such as glass beads and polyurethane foam. All immobilized bioreactors resulted in much better 1,3-propanediol production from waste glycerol in comparison to the suspended cell culture bioreactor. It was also demonstrated that the locally available PS material is as good as the commercially available immobilization material. The maximum volumetric productivity (8.5 g L(-1) h(-1)) was obtained by the PS material, which is 220 % higher than the suspended cell system. Furthermore, the immobilized bioreactor system was much more robust against cell washout even at very low hydraulic retention time values. PMID:23079889

  19. Determination of alternative preservatives in cosmetic products by chromophoric derivatization followed by vortex-assisted liquid-liquid semimicroextraction and liquid chromatography.

    PubMed

    Miralles, Pablo; Vrouvaki, Ilianna; Chisvert, Alberto; Salvador, Amparo

    2016-07-01

    An analytical method for the simultaneous determination of phenethyl alcohol, methylpropanediol, phenylpropanol, caprylyl glycol, and ethylhexylglycerin, which are used as alternative preservatives in cosmetic products, has been developed. The method is based on liquid chromatography with UV spectrophotometric detection after chromophoric derivatization with benzoyl chloride and vortex-assisted liquid-liquid semimicroextraction. Different chromatographic parameters, derivatization conditions, and sample preparation variables were studied. Under optimized conditions, the limits of detection values for the analytes ranged from 0.02 to 0.06µgmL(-1). The method was validated with good recovery values (84-118%) and precision values (3.9-9.5%). It was successfully applied to 10 commercially available cosmetic samples. The good analytical features of the proposed method besides of its environmentally-friendly characteristics, make it useful to carry out the quality control of cosmetic products containing the target compounds as preservative agents. PMID:27154641

  20. Alternatives to the use of antimicrobial agents in pig production: A multi-country expert-ranking of perceived effectiveness, feasibility and return on investment.

    PubMed

    Postma, Merel; Stärk, Katharina D C; Sjölund, Marie; Backhans, Annette; Beilage, Elisabeth Grosse; Lösken, Svenja; Belloc, Catherine; Collineau, Lucie; Iten, Denise; Visschers, Vivianne; Nielsen, Elisabeth O; Dewulf, Jeroen

    2015-03-01

    Nineteen alternatives to antimicrobial agents were ranked on perceived effectiveness, feasibility and return on investment (ROI) from 0 (not effective, not feasible, no ROI) to 10 (fully effective, completely feasible, maximum ROI) by 111 pig health experts from Belgium, Denmark, France, Germany, Sweden and Switzerland. The top 5 measures in terms of perceived effectiveness were (1) improved internal biosecurity, (2) improved external biosecurity, (3) improved climate/environmental conditions, (4) high health/Specific Pathogen Free/disease eradication and (5) increased vaccination. The top 5 measures in terms of perceived feasibility were (1) increased vaccination, (2) increased use of anti-inflammatory products, (3) improved water quality, (4) feed quality/optimization and (5) use of zinc/metals. The top 5 measures in terms of perceived ROI were (1) improved internal biosecurity, (2) zinc/metals, (3) diagnostics/action plan, (4) feed quality/optimization and (5) climate/environmental improvements. Univariate linear regression showed that veterinary practitioners rank internal biosecurity, vaccination, use of zinc/metals, feed quality optimization and climate/environmental on average highest, while researchers and professors focused more on increased use of diagnostics and action plans. Financial incentives/penalties ranked low in all countries. Belgian respondents ranked feed quality significantly lower compared to the German respondents while reduction of stocking density was ranked higher in Belgium compared to Denmark. Categorical Principal Component Analysis applied to the average ranking supported the finding that veterinary practitioners had a preference for more practical, common and already known alternatives. The results showed that improvements in biosecurity, increased use of vaccination, use of zinc/metals, feed quality improvement and regular diagnostic testing combined with a clear action plan were perceived to be the most promising alternatives to

  1. Characterization of an Sf-rhabdovirus-negative Spodoptera frugiperda cell line as an alternative host for recombinant protein production in the baculovirus-insect cell system.

    PubMed

    Maghodia, Ajay B; Geisler, Christoph; Jarvis, Donald L

    2016-06-01

    Cell lines derived from the fall armyworm, Spodoptera frugiperda (Sf), are widely used as hosts for recombinant protein production in the baculovirus-insect cell system (BICS). However, it was recently discovered that these cell lines are contaminated with a virus, now known as Sf-rhabdovirus [1]. The detection of this adventitious agent raised a potential safety issue that could adversely impact the BICS as a commercial recombinant protein production platform. Thus, we examined the properties of Sf-RVN, an Sf-rhabdovirus-negative Sf cell line, as a potential alternative host. Nested RT-PCR assays showed Sf-RVN cells had no detectable Sf-rhabdovirus over the course of 60 passages in continuous culture. The general properties of Sf-RVN cells, including their average growth rates, diameters, morphologies, and viabilities after baculovirus infection, were virtually identical to those of Sf9 cells. Baculovirus-infected Sf-RVN and Sf9 cells produced equivalent levels of three recombinant proteins, including an intracellular prokaryotic protein and two secreted eukaryotic glycoproteins, and provided similar N-glycosylation patterns. In fact, except for the absence of Sf-rhabdovirus, the only difference between Sf-RVN and Sf9 cells was SF-RVN produced higher levels of infectious baculovirus progeny. These results show Sf-RVN cells can be used as improved, alternative hosts to circumvent the potential safety hazard associated with the use of Sf-rhabdovirus-contaminated Sf cells for recombinant protein manufacturing with the BICS. PMID:26923062

  2. Arbuscular mycorrhizal fungi are an alternative to the application of chemical fertilizer in the production of the medicinal and aromatic plant Coriandrum sativum L.

    PubMed

    Oliveira, Rui S; Ma, Ying; Rocha, Inês; Carvalho, Maria F; Vosátka, Miroslav; Freitas, Helena

    2016-01-01

    The widespread use of agrochemicals is detrimental to the environment and may exert harmful effects on human health. The consumer demand for organic food plants has been increasing. There is thus a rising need for alternatives to agrochemicals that can foster sustainable plant production. The aim of this study was to evaluate the potential use of an arbuscular mycorrhizal (AM) fungus as an alternative to application of chemical fertilizer for improving growth performance of the medicinal and aromatic plant Coriandrum sativum. Plants were inoculated with the AM fungus Rhizophagus irregularis BEG163 and/or supplemented with a commercial chemical fertilizer (Plant Marvel, Nutriculture Bent Special) in agricultural soil. Plant growth, nutrition, and development of AM fungus were assessed. Plants inoculated with R. irregularis and those supplemented with chemical fertilizer displayed significantly improved growth performances when compared with controls. There were no significant differences in total fresh weight between plants inoculated with R. irregularis or those supplemented with chemical fertilizer. Leaf chlorophyll a + b (82%), shoot nitrogen (44%), phosphorus (254%), and potassium (27%) concentrations increased in plants inoculated with R. irregularis compared to controls. Application of chemical fertilizer inhibited root mycorrhizal colonization and the length of the extraradical mycelium of R. irregularis. Inoculation with R. irregularis was equally or more efficient than application of chemical fertilizer in promoting growth and nutrition of C. sativum. AM fungi may thus contribute to improve biologically based production of food plants and reduce the dependence on agrochemicals in agriculture. PMID:27077563

  3. Characterizing 13 Years of Surface Water Variability from MODIS-based Near Real-Time Flood Mapping Products in the Indus River, Tonle Sap Lake, and Lake Chad.

    NASA Astrophysics Data System (ADS)

    Slayback, D. A.; Brakenridge, G. R.; Policelli, F. S.

    2015-12-01

    Driven by an increase in extreme weather events in a warming world, flooding appears to be increasing in many regions. Since 2012, we have been using the twice-daily near-global observations of the two MODIS instruments to operate a near real-time flood mapping capability. Primarily intended to support disaster response efforts, our system generates daily near-global maps of flood water extent, at 250 m resolution. Although cloud cover is a challenge, the twice-daily coverage from the Terra and Aqua satellites helps to capture most major events. We use the MOD44W product (the "MODIS 250-m land-water mask") to differentiate "normal" water from flood water. Products from the system are freely available, and used by disaster response agencies and academic and industry researchers. An open question, however, is: how "normal" are recently observed floods? Destructive and — as reported by the press — record floods seem to be occurring more and more frequently. With the MODIS archive going back to 1999 (Terra satellite) and 2002 (Aqua satellite), we now have more than a decade of twice-daily near-global observations to begin answering this question. Although the 13 years of available twice-daily data (2002-2015) are not sufficient to fully characterize surface water normals (e.g., 100-year floods), we can start examining recent trends in surface water extent and flood frequency. To do so, we have back-processed our surface water product through mid-2002 (Aqua launch) for a few regions, and have used this to evaluate the variability in surface water extent and flood frequency. These results will eventually feed back into an improved characterization of flood water in our near real-time flood product. Here we will present results on trends in surface water extent and flood frequency for a few regions, including the Indus in Pakistan, the Tonle Sap lake in Cambodia, and lake Chad in Africa.

  4. Characterization of a real time H2O2 monitor for use in studies on H2O2 production by antibodies and cells.

    PubMed

    Sharma, Harish A; Balcavage, Walter X; Waite, Lee R; Johnson, Mary T; Nindl, Gabi

    2003-01-01

    It was recently shown that antibodies catalyze a reaction between water and ultraviolet light (UV) creating singlet oxygen and ultimately H2O2. Although the in vivo relevance of these antibody reactions is unclear, it is interesting that among a wide variety of non-antibody proteins tested, the T cell receptor is the only protein with similar capabilities. In clinical settings UV is believed to exert therapeutic effects by eliminating inflammatory epidermal T cells and we hypothesized that UV-triggered H2O2 production is involved in this process. To test the hypothesis we developed tools to study production of H2O2 by T cell receptors with the long-term goal of understanding, and improving, UV phototherapy. Here, we report the development of an inexpensive, real time H2O2 monitoring system having broad applicability. The detector is a Clark oxygen electrode (Pt, Ag/AgCl) modified to detect UV-driven H2O2 production. Modifications include painting the electrode black to minimize UV effects on the Ag/AgCl electrode and the use of hydrophilic, large pore Gelnots electrode membranes. Electrode current was converted to voltage and then amplified and recorded using a digital multimeter coupled to a PC. A reaction vessel with a quartz window was developed to maintain constant temperature while permitting UV irradiation of the samples. The sensitivity and specificity of the system and its use in cell-free and cell-based assays will be presented. In a cellfree system, production of H2O2 by CD3 antibodies was confirmed using our real time H2O2 monitoring method. Additionally we report the finding that splenocytes and Jurkat T cells also produce H2O2 when exposed to UV light. PMID:12724951

  5. Alternative Parameterization of the 3-PG Model for Loblolly Pine: A Regional Validation and Climate Change Assessment on Stand Productivity

    NASA Astrophysics Data System (ADS)

    Yang, J.; Gonzalez-Benecke, C. A.; Teskey, R. O.; Martin, T.; Jokela, E. J.

    2015-12-01

    Loblolly pine (Pinus taeda L.) is one of the fastest growing pine species. It has been planted on more than 10 million ha in the southeastern U.S., and also been introduced into many countries. Using data from the literature and long-term productivity studies, we re-parameterized the 3-PG model for loblolly pine stands. We developed new functions for estimating NPP allocation dynamics, canopy cover and needlefall dynamics, effects of frost on production, density-independent and density-dependent tree mortality, biomass pools at variable starting ages, and the fertility rating. New functions to estimate merchantable volume partitioning were also included, allowing for economic analyses. The fertility rating was determined as a function of site index (mean height of dominant trees at age=25 years). We used the largest and most geographically extensive validation dataset for this species ever used (91 pots in 12 states in U.S. and 10 plots in Uruguay). Comparison of modeled to measured data showed robust agreement across the natural range in the U.S., as well as in Uruguay, where the species is grown as an exotic. Using the new set of functions and parameters with downscaled projections from twenty different climate models, the model was applied to assess the impact of future climate change scenarios on stand productivity in the southeastern U.S.

  6. Mandarin-speaking three-year-olds' demonstration of productive knowledge of syntax: evidence from syntactic productivity and structural priming with the SVO-ba alternation.

    PubMed

    Hsu, Dong-Bo

    2014-09-01

    Two studies investigated syntactic productivity in three-year-old Mandarin speakers' use of verbs in the SVO and SbaOV constructions. In Study 1, children were taught novel verbs in one construction and assessed for their production in the other construction. Children produced verbs taught in the ba constructions in SVO utterances, but showed order effects when producing verbs taught in SVO constructions in ba utterances. In Study 2, children described animated scenes either with structural priming (i.e., after hearing verbs in SVO or ba constructions). Children demonstrated structural priming, producing more SVO and ba utterances, respectively, directly after hearing verbs in these constructions. These results indicate that Mandarin speaking three-year-olds demonstrate productive knowledge of both SVO and SbaOV constructions. Their ability to override the predominant input frequency of SVO runs counter to a purely usage-based account of early acquisition of grammar. PMID:24160845

  7. A Review of the Toxicity of HIV Medications II: Interactions with Drugs and Complementary and Alternative Medicine Products.

    PubMed

    Stolbach, Andrew; Paziana, Karolina; Heverling, Harry; Pham, Paul

    2015-09-01

    For many patients today, HIV has become a chronic disease. For those patients who have access to and adhere to lifelong antiretroviral (ARV) therapy, the potential for drug-drug interactions has become a real and life-threatening concern. It is known that most ARV drug interactions occur through the cytochrome P450 (CYP) pathway. Medications for comorbid medical conditions, holistic supplements, and illicit drugs can be affected by CYP inhibitors and inducers and have the potential to cause harm and toxicity. Protease inhibitors (PIs) tend to inhibit CYP3A4, while most non-nucleoside reverse transcriptase inhibitors (NNRTIs) tend to induce the enzyme. As such, failure to adjust the dose of co-administered medications, such as statins and steroids, may lead to serious complications including rhabdomyolysis and hypercortisolism, respectively. Similarly, gastric acid blockers can decrease several ARV absorption, and warfarin doses may need to be adjusted to maintain therapeutic concentrations. Illicit drugs such as methylenedioxymethamphetamine (MDMA, "ecstasy") in combination with PIs lead to increased toxicity, while the concomitant administration of sedative drugs such as midazolam and alprazolam in patients taking PIs can result in prolonged sedation, delayed recovery, and increased length of stay. Even supplements like St. John's Wort can alter PI concentrations. In theory, any drug that is metabolized by CYP has potential for a pharmacokinetic drug-drug interaction with all PIs, cobicistat, and most NNRTIs. When adding a new medication to an ARV regimen, use of a drug-drug interaction software and/or consultation with a clinical pharmacist/pharmacologist or HIV specialist is recommended. PMID:26036354

  8. Energy balances in the production and end use of alcohols derived from biomass. A fuels-specific comparative analysis of alternate ethanol production cycles

    NASA Astrophysics Data System (ADS)

    1980-10-01

    Questions of net gains in premium fuels that can be derived from the production and use of ethanol from biomass, and that for the US alcohol fuel program, energy balance need not be a concern. Three categories of fuel gain are discussed in the report: (1) net petroleum gain; (2) net premium fuel gain (petroleum and natural gas); and (3) net energy gain (for all fuels). In this study the investment of energy (in the form of premium fuels) in alcohol production includes all investment from cultivating, harvesting, or gathering the feedstock and raw materials, through conversion of the feedstock to alcohol, to the delivery to the end user.

  9. An alternative splicing product of the murine trpv1 gene dominant negatively modulates the activity of TRPV1 channels.

    PubMed

    Wang, Chunbo; Hu, Hong-Zhen; Colton, Craig K; Wood, Jackie D; Zhu, Michael X

    2004-09-01

    Transient receptor potential vanilloid 1 (TRPV1), or vanilloid receptor 1, is the founding member of the vanilloid type of TRP superfamily of nonselective cation channels. TRPV1 is activated by noxious heat, acid, and alkaloid irritants as well as several endogenous ligands and is sensitized by inflammatory factors, thereby serving important functions in detecting noxious stimuli in the sensory system and pathological states in different parts of the body. Whereas numerous studies have been carried out using the rat and human TRPV1 cDNA, the mouse TRPV1 cDNA has not been characterized. Here, we report molecular cloning of two TRPV1 cDNA variants from dorsal root ganglia of C57BL/6 mice. The deduced proteins are designated TRPV1alpha and TRPV1beta and contain 839 and 829 amino acids, respectively. TRPV1beta arises from an alternative intron recognition signal within exon 7 of the trpv1 gene. We found a predominant expression of TRPV1alpha in many tissues and significant expression of TRPV1beta in dorsal root ganglia, skin, stomach, and tongue. When expressed in HEK 293 cells or Xenopus oocytes, TRPV1alpha formed a Ca(2+)-permeable channel activated by ligands known to stimulate TRPV1. TRPV1beta was not functional by itself but its co-expression inhibited the function of TRPV1alpha. Furthermore, although both isoforms were synthesized at a similar rate, less TRPV1beta than TRPV1alpha protein was found in cells and on the cell surface, indicating that the beta isoform is highly unstable. Our data suggest that TRPV1beta is a naturally occurring dominant-negative regulator of the responses of sensory neurons to noxious stimuli. PMID:15234965

  10. Production of activated carbon from biodiesel solid residues: An alternative for hazardous metal sorption from aqueous solution.

    PubMed

    Ribeiro, Rita F L; Soares, Vitor C; Costa, Letícia M; Nascentes, Clésia C

    2015-10-01

    In this study, the potential for the sorption of Pb(2+) and Cd(2+) from aqueous solutions using HNO3-treated activated carbon (TAC) obtained from radish press cake (Raphanus sativus L.), a solid residue from biodiesel production, was investigated. Activated carbon (AC) was obtained by physical activation with CO2(g). Chemical modification with HNO3 was employed to increase the sorption capability of the AC. The sorption of Pb(2+) and Cd(2+) was studied in monometallic systems in equilibrium with different metal-ion concentrations (10-400 mg L(-1)). The experimental sorption equilibrium data were fit to the Langmuir and Freundlich isotherm models. The maximum sorption capacity (qmax) obtained for AC from the Langmuir isotherm was 45.5 mg g(-1) for Cd(2+) and 250 mg g(-1) for Pb(2+). Moreover, TAC presented qmax of 166.7 mg g(-1) (1.48 mmol g(-1)) for Cd(2+) and 500.0 mg g(-1) (2.41 mmol g(-1)) for Pb(2+)showing the effect of chemical modification. Sorption-desorption studies showed that the interaction between metals and TAC is reversible and this sorbent can be reused for several consecutive cycles. Furthermore, the sorption of Cd(2+) and Pb(2+) by TAC was not affected by the presence of competing ions. The experimental data obtained in this study indicated that this solid residue is viable for the production of sorbents that remove metals, such as cadmium and lead, from wastewaters and thereby contribute to the sustainable development of the production of biodiesel. PMID:26233585

  11. Real Language Meets Real Business

    ERIC Educational Resources Information Center

    Muirhead, Muirhead; Schechter, Sarah

    2016-01-01

    The Real Business Language Challenge was a collaborative pilot project between Coca-Cola Enterprises (CCE) and Routes into Languages East for Year 9 and 10 pupils. It was based on CCE's award-winning Real Business Challenge, part of its highly acclaimed education programme. The Real Business Language Challenge transformed the project into a…

  12. Transportation Energy Futures Series: Alternative Fuel Infrastructure Expansion: Costs, Resources, Production Capacity, and Retail Availability for Low-Carbon Scenarios

    SciTech Connect

    Melaina, M. W.; Heath, G.; Sandor, D.; Steward, D.; Vimmerstedt, L.; Warner, E.; Webster, K. W.

    2013-04-01

    Achieving the Department of Energy target of an 80% reduction in greenhouse gas emissions by 2050 depends on transportation-related strategies combining technology innovation, market adoption, and changes in consumer behavior. This study examines expanding low-carbon transportation fuel infrastructure to achieve deep GHG emissions reductions, with an emphasis on fuel production facilities and retail components serving light-duty vehicles. Three distinct low-carbon fuel supply scenarios are examined: Portfolio: Successful deployment of a range of advanced vehicle and fuel technologies; Combustion: Market dominance by hybridized internal combustion engine vehicles fueled by advanced biofuels and natural gas; Electrification: Market dominance by electric drive vehicles in the LDV sector, including battery electric, plug-in hybrid, and fuel cell vehicles, that are fueled by low-carbon electricity and hydrogen. A range of possible low-carbon fuel demand outcomes are explored in terms of the scale and scope of infrastructure expansion requirements and evaluated based on fuel costs, energy resource utilization, fuel production infrastructure expansion, and retail infrastructure expansion for LDVs. This is one of a series of reports produced as a result of the Transportation Energy Futures (TEF) project, a Department of Energy-sponsored multi-agency project initiated to pinpoint underexplored transportation-related strategies for abating GHGs and reducing petroleum dependence.

  13. Modeling the influence of alternative forest management scenarios on wood production and carbon storage: A case study in the Mediterranean region.

    PubMed

    Bottalico, Francesca; Pesola, Lucia; Vizzarri, Matteo; Antonello, Leonardo; Barbati, Anna; Chirici, Gherardo; Corona, Piermaria; Cullotta, Sebastiano; Garfì, Vittorio; Giannico, Vincenzo; Lafortezza, Raffaele; Lombardi, Fabio; Marchetti, Marco; Nocentini, Susanna; Riccioli, Francesco; Travaglini, Davide; Sallustio, Lorenzo

    2016-01-01

    Forest ecosystems are fundamental for the terrestrial biosphere as they deliver multiple essential ecosystem services (ES). In environmental management, understanding ES distribution and interactions and assessing the economic value of forest ES represent future challenges. In this study, we developed a spatially explicit method based on a multi-scale approach (MiMoSe-Multiscale Mapping of ecoSystem services) to assess the current and future potential of a given forest area to provide ES. To do this we modified and improved the InVEST model in order to adapt input data and simulations to the context of Mediterranean forest ecosystems. Specifically, we integrated a GIS-based model, scenario model, and economic valuation to investigate two ES (wood production and carbon sequestration) and their trade-offs in a test area located in Molise region (Central Italy). Spatial information and trade-off analyses were used to assess the influence of alternative forest management scenarios on investigated services. Scenario A was designed to describe the current Business as Usual approach. Two alternative scenarios were designed to describe management approaches oriented towards nature protection (scenario B) or wood production (scenario C) and compared to scenario A. Management scenarios were simulated at the scale of forest management units over a 20-year time period. Our results show that forest management influenced ES provision and associated benefits at the regional scale. In the test area, the Total Ecosystem Services Value of the investigated ES increases 85% in scenario B and decreases 82% in scenario C, when compared to scenario A. Our study contributes to the ongoing debate about trade-offs and synergies between carbon sequestration and wood production benefits associated with socio-ecological systems. The MiMoSe approach can be replicated in other contexts with similar characteristics, thus providing a useful basis for the projection of benefits from forest

  14. Enumeration of Salmonellae in Table Eggs, Pasteurized Egg Products, and Egg-Containing Dishes by Using Quantitative Real-Time PCR

    PubMed Central

    Jakočiūnė, Džiuginta; Pasquali, Frédérique; da Silva, Cristiana Soares; Löfström, Charlotta; Hoorfar, Jeffrey; Klein, Günter; Manfreda, Gerardo

    2014-01-01

    Salmonellae are a major cause of food-borne outbreaks in Europe, with eggs and egg products being identified as major sources. Due to the low levels of salmonellae in eggs and egg products, direct quantification is difficult. In the present study, enrichment quantitative real-time PCR (qPCR) was employed for enumeration of salmonellae in different matrices: table eggs, pasteurized egg products, and egg-containing dishes. Salmonella enterica serovar Enteritidis and S. enterica serovar Tennessee were used to artificially contaminate these matrices. The results showed a linear regression between the numbers of salmonellae and the quantification cycle (Cq) values for all matrices used, with the exception of pasteurized egg white. Standard curves were constructed by using both stationary-phase cells and heat-stressed cells, with similar results. Finally, this method was used to evaluate the fate of salmonellae in two egg-containing dishes, long egg and tiramisu, at abused refrigeration temperatures, and results indicated the growth of bacteria over a 1-week period. In conclusion, enrichment qPCR was shown to be reliable for enumeration of salmonellae in different egg products. PMID:24362433

  15. Enumeration of salmonellae in table eggs, pasteurized egg products, and egg-containing dishes by using quantitative real-time PCR.

    PubMed

    Jakociune, Dziuginta; Pasquali, Frédérique; da Silva, Cristiana Soares; Löfström, Charlotta; Hoorfar, Jeffrey; Klein, Günter; Manfreda, Gerardo; Olsen, John Elmerdahl

    2014-03-01

    Salmonellae are a major cause of food-borne outbreaks in Europe, with eggs and egg products being identified as major sources. Due to the low levels of salmonellae in eggs and egg products, direct quantification is difficult. In the present study, enrichment quantitative real-time PCR (qPCR) was employed for enumeration of salmonellae in different matrices: table eggs, pasteurized egg products, and egg-containing dishes. Salmonella enterica serovar Enteritidis and S. enterica serovar Tennessee were used to artificially contaminate these matrices. The results showed a linear regression between the numbers of salmonellae and the quantification cycle (Cq) values for all matrices used, with the exception of pasteurized egg white. Standard curves were constructed by using both stationary-phase cells and heat-stressed cells, with similar results. Finally, this method was used to evaluate the fate of salmonellae in two egg-containing dishes, long egg and tiramisu, at abused refrigeration temperatures, and results indicated the growth of bacteria over a 1-week period. In conclusion, enrichment qPCR was shown to be reliable for enumeration of salmonellae in different egg products. PMID:24362433

  16. Direct analysis in real time-mass spectrometry (DART-MS) for rapid qualitative screening of toxic glycols in glycerin-containing products.

    PubMed

    Self, Randy L

    2013-06-01

    In 2007, the United States Food and Drug Administration released guidance recommending testing of glycerin used in regulated consumer products, such as cough syrup preparations, toothpaste, and other pharmaceutical and food products, for the toxic compounds ethylene glycol and diethylene glycol. Regulatory laboratories routinely test glycerin, and products containing glycerin or related compounds for these toxic glycols, using an official gas chromatographic method, to ensure the safety of these products. The current work describes a companion technique to compliment this GC-FID method utilizing Orbitrap mass spectrometry with direct analysis in real time ionization to rapidly screen these samples qualitatively, with results in as little as five seconds, with no sample preparation required. This allows the more time and resource intensive method to be reserved for those rare cases when these compounds are detected, potentially greatly improving laboratory efficiency. The technique was evaluated for qualitative sensitivity and repeatability, and compared against the GC-FID method. The method appears to perform well against these metrics. PMID:23584076

  17. The Effectiveness of Vowel Production Training with Real-Time Spectrographic Displays for Children with Profound Hearing Impairment.

    NASA Astrophysics Data System (ADS)

    Ertmer, David Joseph

    1994-01-01

    The effectiveness of vowel production training which incorporated direct instruction in combination with spectrographic models and feedback was assessed for two children who exhibited profound hearing impairment. A multiple-baseline design across behaviors, with replication across subjects was implemented to determine if vowel production accuracy improved following the introduction of treatment. Listener judgments of vowel correctness were obtained during the baseline, training, and follow-up phases of the study. Data were analyzed through visual inspection of changes in levels of accuracy, changes in trends of accuracy, and changes in variability of accuracy within and across phases. One subject showed significant improvement of all three trained vowel targets; the second subject for the first trained target only (Kolmogorov-Smirnov Two Sample Test). Performance trends during training sessions suggest that continued treatment would have resulted in further improvement for both subjects. Vowel duration, fundamental frequency, and the frequency locations of the first and second formants were measured before and after training. Acoustic analysis revealed highly individualized changes in the frequency locations of F1 and F2. Vowels which received the most training were maintained at higher levels than those which were introduced later in training, Some generalization of practiced vowel targets to untrained words was observed in both subjects. A bias towards judging productions as "correct" was observed for both subjects during self-evaluation tasks using spectrographic feedback.

  18. Use of pet food-grade poultry by-product meal as an alternate protein source in weanling pig diets.

    PubMed

    Zier, C E; Jones, R D; Azain, M J

    2004-10-01

    Three experiments were conducted to evaluate pet food-grade poultry by-product meal (PBM) as a replacement protein source for fish meal (FM), blood meal (BM), and spray-dried plasma protein (SDPP) in weanling pig diets. In the first study, 200 crossbred pigs (initial BW = 6.5 kg) were weaned (21 d) and randomly allotted to one of four dietary treatments, which included a control and three test diets where PBM was substituted for FM, blood products, or both. Experimental diets were fed during Phase I (d 0 to 5 postweaning) and Phase II (d 5 to 19), and a common Phase III diet was fed from d 19 to 26. Overall (d 0 to 26), there was no difference in performance of pigs fed PBM in place of the other ingredients. However, during Phase I, BW (P < 0.05), ADG (P < 0.02), and intake (P < 0.001) in pigs fed diets containing SDPP were greater than those fed diets with PBM. In Exp. 2, the performance of pigs (n = 100, initial BW = 6.5 kg) fed diets containing 20% PBM (as-fed basis, replacing SDPP, BM, FM, and a portion of the soybean meal) in all phases of the nursery diet was compared with a group fed conventional diets without PBM. There were no differences in overall performance (d 0 to 26); however, ADG (P < 0.10) and feed intake were higher (P < 0.01) for pigs fed the conventional diet than for pigs fed the 20% PBM diet during Phase I (d 0 to 5). Experiment 3 was a slope-ratio assay to determine the ability of PBM to replace SDPP. A total of 320 pigs (initial BW = 7.32 kg) was weaned (21 d) and allotted to five treatment groups in three trials in a blocked design with product (SDPP or PBM) as the first factor, and lysine level (1.08, 1.28, 1.49%; as-fed basis) as the second factor. Growth rate increased with increasing lysine (P < 0.05), regardless of the source. These results indicate that PBM can be used in nursery diets in place of blood meal and fish meal without affecting performance. Furthermore, although feeding PBM in Phase I diets was not equivalent to SDPP

  19. A real-time regional adaptive exposure method for saving dose-area product in x-ray fluoroscopy

    PubMed Central

    Burion, Steve; Speidel, Michael A.; Funk, Tobias

    2013-01-01

    Purpose: Reduction of radiation dose in x-ray imaging has been recognized as a high priority in the medical community. Here the authors show that a regional adaptive exposure method can reduce dose-area product (DAP) in x-ray fluoroscopy. The authors' method is particularly geared toward providing dose savings for the pediatric population. Methods: The scanning beam digital x-ray system uses a large-area x-ray source with 8000 focal spots in combination with a small photon-counting detector. An imaging frame is obtained by acquiring and reconstructing up to 8000 detector images, each viewing only a small portion of the patient. Regional adaptive exposure was implemented by varying the exposure of the detector images depending on the local opacity of the object. A family of phantoms ranging in size from infant to obese adult was imaged in anteroposterior view with and without adaptive exposure. The DAP delivered to each phantom was measured in each case, and noise performance was compared by generating noise arrays to represent regional noise in the images. These noise arrays were generated by dividing the image into regions of about 6 mm2, calculating the relative noise in each region, and placing the relative noise value of each region in a one-dimensional array (noise array) sorted from highest to lowest. Dose-area product savings were calculated as the difference between the ratio of DAP with adaptive exposure to DAP without adaptive exposure. The authors modified this value by a correction factor that matches the noise arrays where relative noise is the highest to report a final dose-area product savings. Results: The average dose-area product saving across the phantom family was (42 ± 8)% with the highest dose-area product saving in the child-sized phantom (50%) and the lowest in the phantom mimicking an obese adult (23%). Conclusions: Phantom measurements indicate that a regional adaptive exposure method can produce large DAP savings without compromising the

  20. A real-time regional adaptive exposure method for saving dose-area product in x-ray fluoroscopy

    SciTech Connect

    Burion, Steve; Funk, Tobias; Speidel, Michael A.

    2013-05-15

    Purpose: Reduction of radiation dose in x-ray imaging has been recognized as a high priority in the medical community. Here the authors show that a regional adaptive exposure method can reduce dose-area product (DAP) in x-ray fluoroscopy. The authors' method is particularly geared toward providing dose savings for the pediatric population. Methods: The scanning beam digital x-ray system uses a large-area x-ray source with 8000 focal spots in combination with a small photon-counting detector. An imaging frame is obtained by acquiring and reconstructing up to 8000 detector images, each viewing only a small portion of the patient. Regional adaptive exposure was implemented by varying the exposure of the detector images depending on the local opacity of the object. A family of phantoms ranging in size from infant to obese adult was imaged in anteroposterior view with and without adaptive exposure. The DAP delivered to each phantom was measured in each case, and noise performance was compared by generating noise arrays to represent regional noise in the images. These noise arrays were generated by dividing the image into regions of about 6 mm{sup 2}, calculating the relative noise in each region, and placing the relative noise value of each region in a one-dimensional array (noise array) sorted from highest to lowest. Dose-area product savings were calculated as the difference between the ratio of DAP with adaptive exposure to DAP without adaptive exposure. The authors modified this value by a correction factor that matches the noise arrays where relative noise is the highest to report a final dose-area product savings. Results: The average dose-area product saving across the phantom family was (42 {+-} 8)% with the highest dose-area product saving in the child-sized phantom (50%) and the lowest in the phantom mimicking an obese adult (23%). Conclusions: Phantom measurements indicate that a regional adaptive exposure method can produce large DAP savings without

  1. Tobacco BY-2 cell-free lysate: an alternative and highly-productive plant-based in vitro translation system

    PubMed Central

    2014-01-01

    Background Cell-free protein synthesis is a rapid and efficient method for the production of recombinant proteins. Usage of prokaryotic cell-free extracts often leads to non-functional proteins. Eukaryotic counterparts such as wheat germ extract (WGE) and rabbit reticulocyte lysate (RLL) may improve solubility and promote the correct folding of eukaryotic multi-domain proteins that are difficult to express in bacteria. However, the preparation of WGEs is complex and time-consuming, whereas RLLs suffer from low yields. Here we report the development of a novel cell-free system based on tobacco Bright Yellow 2 (BY-2) cells harvested in the exponential growth phase. Results The highly-productive BY-2 lysate (BYL) can be prepared quickly within 4–5 h, compared to 4–5 d for WGE. The efficiency of the BYL was tested using three model proteins: enhanced yellow fluorescent protein (eYFP) and two versions of luciferase. The added mRNA was optimized by testing different 5’ and 3’ untranslated regions (UTRs). The protein yield in batch and dialysis reactions using BYL was much higher than that of a commercial Promega WGE preparation, achieving a maximum yield of 80 μg/mL of eYFP and 100 μg/mL of luciferase, compared to only 45 μg/mL of eYFP and 35 μg/mL of luciferase in WGEs. In dialysis reactions, the BYL yielded about 400 μg/mL eYFP, representing up to 50% more of the target protein than the Promega WGE, and equivalent to the amount using 5Prime WGE system. Conclusions Due to the high yield and the short preparation time the BYL represents a remarkable improvement over current eukaryotic cell-free systems. PMID:24886601

  2. The Impact of Using Alternative Forages on the Nutrient Value within Slurry and Its Implications for Forage Productivity in Agricultural Systems

    PubMed Central

    Crotty, Felicity V.; Fychan, Rhun; Theobald, Vince J.; Sanderson, Ruth; Chadwick, David R.; Marley, Christina L.

    2014-01-01

    Alternative forages can be used to provide valuable home-grown feed for ruminant livestock. Utilising these different forages could affect the manure value and the implications of incorporating these forages into farming systems, needs to be better understood. An experiment tested the hypothesis that applying slurries from ruminants, fed ensiled red clover (Trifolium pratense), lucerne (Medicago sativa) or kale (Brassica oleracea) would improve the yield of hybrid ryegrass (Lolium hybridicum), compared with applying slurries from ruminants fed ensiled hybrid ryegrass, or applying inorganic N alone. Slurries from sheep offered one of four silages were applied to ryegrass plots (at 35 t ha−1) with 100 kg N ha−1 inorganic fertiliser; dry matter (DM) yield was compared to plots only receiving ammonium nitrate at rates of 0, 100 and 250 kg N ha−1 year−1. The DM yield of plots treated with 250 kg N, lucerne or red clover slurry was significantly higher than other treatments (P<0.001). The estimated relative fertiliser N equivalence (FNE) (fertiliser-N needed to produce same yield as slurry N), was greatest for lucerne (114 kg) >red clover (81 kg) >kale (44 kg) >ryegrass (26 kg ha−1 yr−1). These FNE values represent relative efficiencies of 22% (ryegrass), 52% (kale), 47% (red clover) and 60% for lucerne slurry, with the ryegrass slurry efficiency being lowest (P = 0.005). Soil magnesium levels in plots treated with legume slurry were higher than other treatments (P<0.001). Overall, slurries from ruminants fed alternative ensiled forages increased soil nutrient status, forage productivity and better N efficiency than slurries from ruminants fed ryegrass silage. The efficiency of fertiliser use is one of the major factors influencing the sustainability of farming systems, these findings highlight the cascade in benefits from feeding ruminants alternative forages, and the need to ensure their value is effectively captured to reduce environmental risks. PMID

  3. The impact of using alternative forages on the nutrient value within slurry and its implications for forage productivity in agricultural systems.

    PubMed

    Crotty, Felicity V; Fychan, Rhun; Theobald, Vince J; Sanderson, Ruth; Chadwick, David R; Marley, Christina L

    2014-01-01

    Alternative forages can be used to provide valuable home-grown feed for ruminant livestock. Utilising these different forages could affect the manure value and the implications of incorporating these forages into farming systems, needs to be better understood. An experiment tested the hypothesis that applying slurries from ruminants, fed ensiled red clover (Trifolium pratense), lucerne (Medicago sativa) or kale (Brassica oleracea) would improve the yield of hybrid ryegrass (Lolium hybridicum), compared with applying slurries from ruminants fed ensiled hybrid ryegrass, or applying inorganic N alone. Slurries from sheep offered one of four silages were applied to ryegrass plots (at 35 t ha⁻¹) with 100 kg N ha⁻¹ inorganic fertiliser; dry matter (DM) yield was compared to plots only receiving ammonium nitrate at rates of 0, 100 and 250 kg N ha⁻¹ year-1. The DM yield of plots treated with 250 kg N, lucerne or red clover slurry was significantly higher than other treatments (P<0.001). The estimated relative fertiliser N equivalence (FNE) (fertiliser-N needed to produce same yield as slurry N), was greatest for lucerne (114 kg) >red clover (81 kg) >kale (44 kg) >ryegrass (26 kg ha⁻¹ yr⁻¹). These FNE values represent relative efficiencies of 22% (ryegrass), 52% (kale), 47% (red clover) and 60% for lucerne slurry, with the ryegrass slurry efficiency being lowest (P = 0.005). Soil magnesium levels in plots treated with legume slurry were higher than other treatments (P<0.001). Overall, slurries from ruminants fed alternative ensiled forages increased soil nutrient status, forage productivity and better N efficiency than slurries from ruminants fed ryegrass silage. The efficiency of fertiliser use is one of the major factors influencing the sustainability of farming systems, these findings highlight the cascade in benefits from feeding ruminants alternative forages, and the need to ensure their value is effectively captured to reduce environmental risks

  4. Finding alternatives to antibiotics

    Technology Transfer Automated Retrieval System (TEKTRAN)

    The spread of antibiotic-resistant pathogens requires new treatments. The availability of new antibiotics has severely declined, and so alternatives to antibiotics need to be considered in both animal agriculture and human medicine. Products for disease prevention are different than products for d...

  5. A Simultaneous Analytical Method for Duplex Identification of Porcine and Horse in the Meat Products by EvaGreen based Real-time PCR.

    PubMed

    Sakalar, Ergün; Ergün, Seyma Özçirak; Akar, Emine

    2015-01-01

    A duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GIDAGEN® Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5℃ and 78℃ for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex real-time PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats. PMID:26761852

  6. A Simultaneous Analytical Method for Duplex Identification of Porcine and Horse in the Meat Products by EvaGreen based Real-time PCR

    PubMed Central

    2015-01-01

    A duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GIDAGEN® Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5℃ and 78℃ for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex real-time PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats. PMID:26761852

  7. Direct enzymatic extraction of starch from corn as an energy saving alternative to production of high fructose syrup. Final executive report

    SciTech Connect

    Not Available

    1982-12-01

    The purpose of this project was to study, test, and demonstrate a process of producing high-fructose corn syrup and protein byproducts from dry milled corn as an energy conserving alternative of the current industrial corn wet-milling process. This final report is divided into 5 sections. Section 1 deals with the process which is the main and, indeed, the final product of the energy conservation study. Section 2 deals with protein Extraction which conditions the dry-milled corn before hydrolysis. Section 3 deals with the analytical technique of GPC developed with the alpha-amylase hydrolysis of starch. Section 4 deals with immobilized glucoamylase hydrolysis. Section 5 deals with the recovery of soluble protein by ion-exchange resins. Each section has been abstracted separately for inclusion in the Energy Data Base. (DMC)

  8. Applications of advanced petroleum production technology and water alternating gas injection for enhanced oil recovery - Mattoon Oil Field, Illinois. Final report

    SciTech Connect

    Baroni, M.

    1995-09-01

    Phase I results of a C0{sub 2}-assisted oil recovery demonstration project in selected Cypress Sandstone reservoirs at Mattoon Field, Illinois are reported. The design and scope of this project included C0{sub 2} injectvity testing in the Pinnell and Sawyer units, well stimulaton treatments with C0{sub 2} in the Strong unit and infill well drilling, completion and oil production. The field activities were supported by extensive C0{sub 2}-oil-water coreflood experiments, CO{sub 2} oil-phase interaction experiments, and integrated geologic modeling and reservoir simulations. The progress of the project was made public through presentations at an industry meeting and a DOEs contractors` symposium, through quarterly reports and one-to-one consultations with interested operators. Phase II of this project was not implemented. It would have been a water-alternating-gas (WAG) project of longer duration.

  9. Alternate carbohydrate and nontraditional inducer leads to increased productivity of a collagen binding domain fusion protein via fed-batch fermentation.

    PubMed

    Fruchtl, McKinzie; Sakon, Joshua; Beitle, Robert

    2016-05-20

    The production of collagen binding domain fusion proteins is of significant importance because of their potential as therapeutic biomaterials. It was previously reported that the expression of collagen-binding domain fusion proteins in Escherichia coli was higher when expressed using lactose as an inducer and chemically defined growth media on a shake flask scale. In an effort to further investigate factors that affect expression levels on a fed-batch scale, alternative induction techniques were tested in conjunction with fed-batch fermentation. In this paper, we discuss ten fed-batch fermentation experiments utilizing either glucose or glycerol feed and using lactose or isopropyl-β-d-thiogalactopyranoside (IPTG) as an induction source. It was found that glycerol-fed fermentations induced with lactose allowed for greater expression of target protein, though lesser cell densities were achieved. PMID:26975843

  10. Seawater as Alternative to Freshwater in Pretreatment of Date Palm Residues for Bioethanol Production in Coastal and/or Arid Areas.

    PubMed

    Fang, Chuanji; Thomsen, Mette Hedegaard; Brudecki, Grzegorz P; Cybulska, Iwona; Frankaer, Christian Grundahl; Bastidas-Oyanedel, Juan-Rodrigo; Schmidt, Jens Ejbye

    2015-11-01

    The large water consumption (1.9-5.9 m(3) water per m(3) of biofuel) required by biomass processing plants has become an emerging concern, which is particularly critical in arid/semiarid regions. Seawater, as a widely available water source, could be an interesting option. This work was to study the technical feasibility of using seawater to replace freshwater in the pretreatment of date palm leaflets, a lignocellulosic biomass from arid regions, for bioethanol production. It was shown that leaflets pretreated with seawater exhibited lower cellulose crystallinity than those pretreated with freshwater. Pretreatment with seawater produced comparably digestible and fermentable solids to those obtained with freshwater. Moreover, no significant difference of inhibition to Saccharomyces cerevisiae was observed between liquids from pretreatment with seawater and freshwater. The results showed that seawater could be a promising alternative to freshwater for lignocellulose biorefineries in coastal and/or arid/semiarid areas. PMID:26487350

  11. Stimuli-Responsive/Rheoreversible Hydraulic Fracturing Fluids as a Greener Alternative to Support Geothermal and Fossil Energy Production

    SciTech Connect

    Jung, Hun Bok; Carroll, KC; Kabilan, Senthil; Heldebrant, David J.; Hoyt, David W.; Zhong, Lirong; Varga, Tamas; Stephens, Sean A.; Adams, Lexor; Bonneville, Alain; Kuprat, Andrew P.; Fernandez, Carlos A.

    2015-01-01

    Cost-effective yet safe creation of high-permeability reservoirs within deep bedrock is the primary challenge for the viability of enhanced geothermal systems (EGS) and unconventional oil/gas recovery. Although fracturing fluids are commonly used for oil/gas, standard fracturing methods are not developed or proven for EGS temperatures and pressures. Furthermore, the environmental impacts of currently used fracturing methods are only recently being determined. Widespread concerns about the environmental contamination have resulted in a number of regulations for fracturing fluids advocating for greener fracturing processes. To enable EGS feasibility and lessen environmental impact of reservoir stimulation, an environmentally benign, CO2-activated, rheoreversible fracturing fluid that enhances permeability through fracturing (at significantly lower effective stress than standard fracturing fluids) due to in situ volume expansion and gel formation is investigated herein. The chemical mechanism, stability, phase-change behavior, and rheology for a novel polyallylamine (PAA)-CO2 fracturing fluid was characterized at EGS temperatures and pressures. Hydrogel is formed upon reaction with CO2 and this process is reversible (via CO2 depressurization or solubilizing with a mild acid) allowing removal from the formation and recycling, decreasing environmental impact. Rock obtained from the Coso geothermal field was fractured in laboratory experiments under various EGS temperatures and pressures with comparison to standard fracturing fluids, and the fractures were characterized with imaging, permeability measurement, and flow modeling. This novel fracturing fluid and process may vastly reduce water usage and the environmental impact of fracturing practices and effectively make EGS production and unconventional oil/gas exploitation cost-effective and cleaner.

  12. Real-Time Simulation

    NASA Technical Reports Server (NTRS)

    1997-01-01

    Coryphaeus Software, founded in 1989 by former NASA electronic engineer Steve Lakowske, creates real-time 3D software. Designer's Workbench, the company flagship product, is a modeling and simulation tool for the development of both static and dynamic 3D databases. Other products soon followed. Activation, specifically designed for game developers, allows developers to play and test the 3D games before they commit to a target platform. Game publishers can shorten development time and prove the "playability" of the title, maximizing their chances of introducing a smash hit. Another product, EasyT, lets users create massive, realistic representation of Earth terrains that can be viewed and traversed in real time. Finally, EasyScene software control the actions among interactive objects within a virtual world. Coryphaeus products are used on Silican Graphics workstation and supercomputers to simulate real-world performance in synthetic environments. Customers include aerospace, aviation, architectural and engineering firms, game developers, and the entertainment industry.

  13. Quantification of bovine casein fractions by direct chromatographic analysis of milk. Approaching the application to a real production context.

    PubMed

    Bonizzi, Ivan; Buffoni, Joanna Natalia; Feligini, Maria

    2009-01-01

    The ability to quantify the casein content by an exact and cost-effective approach represents an issue of crucial importance in the dairy industry as the natural variations in milk protein concentration can markedly affect the yield of the cheesemaking processes, thus causing a direct and significant economic impact on the producers. In this work, the separation and quantification of alpha(s1)-, alpha(s2)-, kappa- and beta-casein was carried out by direct RP-HPLC analysis of milk. The identification of each casein was established by electrospray ionization mass spectrometry. The data show that this method is able to effectively separate the bovine casein fractions, it provides simplified analytical conditions (with special regard to mobile phase composition and gradient profile) and faster separation while ensuring adequate precision to achieve reliable quantifications in milk samples from dairy production. PMID:19062022

  14. Binding and cleavage (BINACLE) assay for the functional in vitro detection of tetanus toxin: applicability as alternative method for the safety testing of tetanus toxoids during vaccine production.

    PubMed

    Behrensdorf-Nicol, Heike A; Bonifas, Ursula; Hanschmann, Kay-Martin; Krämer, Beate; Weißer, Karin

    2013-12-16

    Tetanus toxoids (i.e. chemically inactivated preparations of tetanus neurotoxin) are used for the production of tetanus vaccines. In order to exclude the risk of residual toxicity or of a "reversion to toxicity", each batch of tetanus toxoid is subject to strict safety testing. Up to now, these prescribed safety tests have to be performed as in vivo toxicity tests in guinea pigs. However, as animal tests are generally slow, costly and ethically disputable, a replacement by an in vitro method would be desirable. A suitable alternative method would have to be able to sensitively detect already low concentrations of active tetanus neurotoxin in matrices containing large amounts of inactivated toxoid molecules. We have developed a method which detects active tetanus neurotoxin molecules based on their specific receptor-binding capacity as well as their proteolytic activity. By taking into account two relevant functional characteristics, this combined "BINding And CLEavage" (BINACLE) assay more reliably discriminates between toxic and detoxified molecules than other in vitro assays which solely rely on one single toxin function (e.g. endopeptidase assays). Data from an in-house validation show that the BINACLE assay is able to detect active tetanus neurotoxin with a detection limit comparable to the in vivo test. The sensitive detection of active toxin which has been spiked into toxoid samples from different manufacturers could also be demonstrated. Specificity and precision of the method have been shown to be satisfactory. The presented data indicate that for toxoid batches from some of the most relevant European vaccine manufacturers, the BINACLE assay may represent a potential alternative to the prescribed animal safety tests. In addition, this novel method may also provide a convenient tool for monitoring batch-to-batch consistency during toxoid production. PMID:24156922

  15. Single-Stage Operation of Hybrid Dark-Photo Fermentation to Enhance Biohydrogen Production through Regulation of System Redox Condition: Evaluation with Real-Field Wastewater.

    PubMed

    Chandra, Rashmi; Nikhil, G N; Mohan, S Venkata

    2015-01-01

    Harnessing hydrogen competently through wastewater treatment using a particular class of biocatalyst is indeed a challenging issue. Therefore, biohydrogen potential of real-field wastewater was evaluated by hybrid fermentative process in a single-stage process. The cumulative hydrogen production (CHP) was observed to be higher with distillery wastewater (271 mL) than with dairy wastewater (248 mL). Besides H₂ production, the hybrid process was found to be effective in wastewater treatment. The chemical oxygen demand (COD) removal efficiency was found higher in distillery wastewater (56%) than in dairy wastewater (45%). Co-culturing photo-bacterial flora assisted in removal of volatile fatty acids (VFA) wherein 63% in distillery wastewater and 68% in case of dairy wastewater. Voltammograms illustrated dominant reduction current and low cathodic Tafel slopes supported H₂ production. Overall, the augmented dark-photo fermentation system (ADPFS) showed better performance than the control dark fermentation system (DFS). This kind of holistic approach is explicitly viable for practical scale-up operation. PMID:25927577

  16. Single-Stage Operation of Hybrid Dark-Photo Fermentation to Enhance Biohydrogen Production through Regulation of System Redox Condition: Evaluation with Real-Field Wastewater

    PubMed Central

    Chandra, Rashmi; Nikhil, G. N.; Mohan, S. Venkata

    2015-01-01

    Harnessing hydrogen competently through wastewater treatment using a particular class of biocatalyst is indeed a challenging issue. Therefore, biohydrogen potential of real-field wastewater was evaluated by hybrid fermentative process in a single-stage process. The cumulative hydrogen production (CHP) was observed to be higher with distillery wastewater (271 mL) than with dairy wastewater (248 mL). Besides H2 production, the hybrid process was found to be effective in wastewater treatment. The chemical oxygen demand (COD) removal efficiency was found higher in distillery wastewater (56%) than in dairy wastewater (45%). Co-culturing photo-bacterial flora assisted in removal of volatile fatty acids (VFA) wherein 63% in distillery wastewater and 68% in case of dairy wastewater. Voltammograms illustrated dominant reduction current and low cathodic Tafel slopes supported H2 production. Overall, the augmented dark-photo fermentation system (ADPFS) showed better performance than the control dark fermentation system (DFS). This kind of holistic approach is explicitly viable for practical scale-up operation. PMID:25927577

  17. DNA barcoding, species-specific PCR and real-time PCR techniques for the identification of six Tribolium pests of stored products.

    PubMed

    Zhang, Tao; Wang, Yi-Jiao; Guo, Wei; Luo, Dan; Wu, Yi; Kučerová, Zuzana; Stejskal, Václav; Opit, George; Cao, Yang; Li, Fu-Jun; Li, Zhi-Hong

    2016-01-01

    Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored product pests in China and worldwide. They are often found or are intercepted in grain depots, flour mills, and entry-exit ports, etc. Traditionally, Tribolium species are identified according to the morphological characteristics of the adult. However, it is almost impossible to rapidly identify adult fragments and non-adult stages based on external morphological characteristics. Molecular techniques for the rapid and accurate identification of Tribolium species are required, particularly for pest monitoring and the quarantine of stored products pests. Here, we establish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six stored-product pest Tribolium species including T. castaneum, T. confusum, T. destructor, T. madens, T. freemani and T. brevicornis. We detected the mitochondrial DNA cytochrome oxidase subunit I (COI) barcodes for Tribolium from 18 geographic populations and 101 individuals, built a Tribolium DNA barcode library, and designed species-specific primers and TaqMan probes for the above six Tribolium species. The three techniques were applied to identify Tribolium collected from stored samples and samples captured from quarantine ports. The results demonstrated that three techniques were all able to identify the six species of Tribolium both rapidly and accurately. PMID:27352804

  18. DNA barcoding, species-specific PCR and real-time PCR techniques for the identification of six Tribolium pests of stored products

    PubMed Central

    Zhang, Tao; Wang, Yi-Jiao; Guo, Wei; Luo, Dan; Wu, Yi; Kučerová, Zuzana; Stejskal, Václav; Opit, George; Cao, Yang; Li, Fu-Jun; Li, Zhi-Hong

    2016-01-01

    Flour beetles of the genus Tribolium Macleay (Coleoptera: Tenebrionidae) are important stored product pests in China and worldwide. They are often found or are intercepted in grain depots, flour mills, and entry-exit ports, etc. Traditionally, Tribolium species are identified according to the morphological characteristics of the adult. However, it is almost impossible to rapidly identify adult fragments and non-adult stages based on external morphological characteristics. Molecular techniques for the rapid and accurate identification of Tribolium species are required, particularly for pest monitoring and the quarantine of stored products pests. Here, we establish DNA barcoding, species-specific PCR, and real-time PCR techniques for the identification of six stored-product pest Tribolium species including T. castaneum, T. confusum, T. destructor, T. madens, T. freemani and T. brevicornis. We detected the mitochondrial DNA cytochrome oxidase subunit I (COI) barcodes for Tribolium from 18 geographic populations and 101 individuals, built a Tribolium DNA barcode library, and designed species-specific primers and TaqMan probes for the above six Tribolium species. The three techniques were applied to identify Tribolium collected from stored samples and samples captured from quarantine ports. The results demonstrated that three techniques were all able to identify the six species of Tribolium both rapidly and accurately. PMID:27352804

  19. The chimeric EWS-WT1 gene product in the desmoplastic small round cell tumor: Molecular detection and alternative transcripts

    SciTech Connect

    Gerald, W.; Alava, E. de; Ladanyi, M.

    1994-09-01

    The desmoplastic small round cell tumor (DSRCT) is a recently recognized aggressive type of primitive sarcoma occurring mainly in young males. Previous cytogenetic reports have identified a recurrent translocation in DSRCT, t(11;22)(p13;q12). We have recently shown that this translocation represents a rearrangement between the EWS and WT1 genes, normally located at 22q12 and 11p13, respectively, generating a fusion gene which encodes a chimeric RNA resulting from an in-frame junction of EWS exon 7 to WT1 exon 8. Thus, this chimeric RNA encodes a putative protein in which the RNA-binding domain of EWS is replaced by the three C-terminal zinc fingers of the WT1 DNA-binding domain. We have now assessed the molecular detection of this rearrangement in a panel of 7 DSRCTs and 38 other small round cell tumors, and we have examined the WT1 portion of the chimeric RNA for the presence of the previously reported splice variants of the zinc-finger DNA-binding domain of WT1. Reverse transcriptase PCR (RT-PCR) revealed a single identical product in 4/5 cases tested, including a case in which a t(11;22)(p13;q12) by karyotyping. By Southern blotting, rearrangement of both EWS and WT1 was detectable in 3/6 cases, EWS alone in 1/6, and neither in 2/6. Histologically, the sole sample negative by both methods contained very scanty viable tumor. EWS-WT1 RT-PCR was negative in 16 Wilms` tumors, 12 rhadomyosarcomas, and 10 Ewing`s sarcomas. RT-PCR with splice site-specific primers showed the chimeric EWS-WT1 transcripts to include both splice variants of the zinc-finger domain of WT1, {open_quotes}+KTS{close_quotes} and {open_quotes}-KTS{close_quotes}. The t(11;22)(p13;q12) of DSRCT is most reliably detected by RT-PCR, and results in a specific and structurally highly consistent EWS-WT1 chimeric transcript which may interact with the normal targets of both splice variants of WT1.

  20. [Pollution and alternative energy sources].

    PubMed

    Melino, C

    1989-01-01

    In order to reach higher standards of living, man has always been interested in searching new energy sources. Natural energy from sun, wind and water has been overcame by more sophisticated resources such as coal, vapour, hydroelectricity, natural gas, petroleum, and, at least, nuclear energy. However all these resources present unwanted effects, namely various hazards to man and environment. On this matter society is quering the risk-benefit balance of some energy choices and optimum performance with new safety means to limit dangerousness are being pursued and developed. It is necessary to evaluate carefully every aspect of safety without under-estimating or over-evaluating problems. For each energy source a "real price" has to be paired, even more in the future, since more energy will be required to guarantee the necessary technological progress linked to a better quality of life. In the present review all risks related to different energy sources are described and discussed aiming at defining: 1) specific risks for different sources 2) benefit from their utilization 3) means of defence guaranteeing security for man and environment. Italy is strictly dependent for energy production, which comes for 80% from abroad. An appropriate balance is required considering economical and social factors and real availability of energy. This balance needs therefore to be clearly evaluated hoping in a better future for an alternative energy, less dangerous and more clear, such as that from nuclear fusion. PMID:2483087

  1. Contribution of National near Real Time MODIS Forest Maximum Percentage NDVI Change Products to the U.S. ForWarn System

    NASA Technical Reports Server (NTRS)

    Spruce, Joseph P.; Hargrove, William; Gasser, Gerald; Smoot, James; Kuper, Philip D.

    2012-01-01

    This presentation reviews the development, integration, and testing of Near Real Time (NRT) MODIS forest % maximum NDVI change products resident to the USDA Forest Service (USFS) ForWarn System. ForWarn is an Early Warning System (EWS) tool for detection and tracking of regionally evident forest change, which includes the U.S. Forest Change Assessment Viewer (FCAV) (a publically available on-line geospatial data viewer for visualizing and assessing the context of this apparent forest change). NASA Stennis Space Center (SSC) is working collaboratively with the USFS, ORNL, and USGS to contribute MODIS forest change products to ForWarn. These change products compare current NDVI derived from expedited eMODIS data, to historical NDVI products derived from MODIS MOD13 data. A new suite of forest change products are computed every 8 days and posted to the ForWarn system; this includes three different forest change products computed using three different historical baselines: 1) previous year; 2) previous three years; and 3) all previous years in the MODIS record going back to 2000. The change product inputs are maximum value NDVI that are composited across a 24 day interval and refreshed every 8 days so that resulting images for the conterminous U.S. are predominantly cloud-free yet still retain temporally relevant fresh information on changes in forest canopy greenness. These forest change products are computed at the native nominal resolution of the input reflectance bands at 231.66 meters, which equates to approx 5.4 hectares or 13.3 acres per pixel. The Time Series Product Tool, a MATLAB-based software package developed at NASA SSC, is used to temporally process, fuse, reduce noise, interpolate data voids, and re-aggregate the historical NDVI into 24 day composites, and then custom MATLAB scripts are used to temporally process the eMODIS NDVIs so that they are in synch with the historical NDVI products. Prior to posting, an in-house snow mask classification product

  2. Systematic study of real photon and Drell-Yan pair production in p+A (d+A) interactions

    NASA Astrophysics Data System (ADS)

    Krelina, M.; Basso, E.; Goncalves, V. P.; Nemchik, J.; Pasechnik, R.

    2016-07-01

    We study nuclear effects in production of Drell-Yan pairs and direct photons in proton-nucleus collisions. For the first time, these effects are studied within the color dipole approach using the Green function formalism which naturally incorporates the color transparency and quantum coherence effects. The corresponding numerical results for the nuclear modification factor are compared with available data. Besides, we present a variety of predictions for the nuclear suppression as a function of transverse momentum pT, Feynman variable xF and invariant mass M of the lepton pair which can be verified by experiments at RHIC and LHC. We found that the nuclear suppression is caused predominantly by effects of quantum coherence (shadowing corrections) and by the effective energy loss induced by multiple initial state interactions. Whereas the former dominate at small Bjorken x2 in the target, the latter turns out to be significant at large x1 in the projectile beam and is universal at different energies and transverse momenta.

  3. AirNow Information Management System - Global Earth Observation System of Systems Data Processor for Real-Time Air Quality Data Products

    NASA Astrophysics Data System (ADS)

    Haderman, M.; Dye, T. S.; White, J. E.; Dickerson, P.; Pasch, A. N.; Miller, D. S.; Chan, A. C.

    2012-12-01

    Built upon the success of the U.S. Environmental Protection Agency's (EPA) AirNow program (www.AirNow.gov), the AirNow-International (AirNow-I) system contains an enhanced suite of software programs that process and quality control real-time air quality and environmental data and distribute customized maps, files, and data feeds. The goals of the AirNow-I program are similar to those of the successful U.S. program and include fostering the exchange of environmental data; making advances in air quality knowledge and applications; and building a community of people, organizations, and decision makers in environmental management. In 2010, Shanghai became the first city in China to run this state-of-the-art air quality data management and notification system. AirNow-I consists of a suite of modules (software programs and schedulers) centered on a database. One such module is the Information Management System (IMS), which can automatically produce maps and other data products through the use of GIS software to provide the most current air quality information to the public. Developed with Global Earth Observation System of Systems (GEOSS) interoperability in mind, IMS is based on non-proprietary standards, with preference to formal international standards. The system depends on data and information providers accepting and implementing a set of interoperability arrangements, including technical specifications for collecting, processing, storing, and disseminating shared data, metadata, and products. In particular, the specifications include standards for service-oriented architecture and web-based interfaces, such as a web mapping service (WMS), web coverage service (WCS), web feature service (WFS), sensor web services, and Really Simple Syndication (RSS) feeds. IMS is flexible, open, redundant, and modular. It also allows the merging of data grids to create complex grids that show comprehensive air quality conditions. For example, the AirNow Satellite Data Processor

  4. Social Responsibility in Tobacco Production? Tobacco Companies Use of Green Supply Chains to Obscure the Real Costs of Tobacco Farming

    PubMed Central

    Otañez, Marty

    2011-01-01

    Background Tobacco companies have come under increased criticism because of environmental and labor practices related to growing tobacco in developing countries. Methods Analysis of tobacco industry documents, industry web sites and interviews with tobacco farmers in Tanzania and tobacco farm workers, farm authorities, trade unionists, government officials and corporate executives from global tobacco leaf companies in Malawi. Results British American Tobacco and Philip Morris created supply chains in the 1990s to improve production efficiency, control, access to markets, and profits. In the 2000s, the companies used their supply chains in an attempt to legitimize their portrayals of tobacco farming as socially and environmentally friendly, rather than take meaningful steps to eliminate child labor and reduce deforestation in developing countries. The tobacco companies used nominal self-evaluation (not truly independent evaluators) and public relations to create the impression of social responsibility. The companies benefit from $1.2 billion in unpaid labor costs due to child labor and more than $64 million annually in costs that would have been made to avoid tobacco related deforestation in the top twelve tobacco growing developing countries, far exceeding the money they spend nominally working to change these practices. Conclusions The tobacco industry uses green supply chains to make tobacco farming in developing countries appear sustainable while continuing to purchase leaf produced with child labor and high rates of deforestation. Strategies to counter green supply chain schemes include securing implementing protocols for the WHO Framework Convention on Tobacco Control to regulate the companies’ practices at the farm level. PMID:21504915

  5. Detection of bovine central nervous system tissues in rendered animal by-products by one-step real-time reverse transcription PCR assay.

    PubMed

    Andrievskaia, Olga; Tangorra, Erin

    2014-12-01

    Contamination of rendered animal byproducts with central nervous system tissues (CNST) from animals with bovine spongiform encephalopathy is considered one of the vehicles of disease transmission. Removal from the animal feed chain of CNST originated from cattle of a specified age category, species-labeling of rendered meat products, and testing of rendered products for bovine CNST are tasks associated with the epidemiological control of bovine spongiform encephalopathy. A single-step TaqMan real-time reverse transcriptase (RRT) PCR assay was developed and evaluated for specific detection of bovine glial fibrillary acidic protein (GFAP) mRNA, a biomarker of bovine CNST, in rendered animal by-products. An internal amplification control, mammalian b -actin mRNA, was coamplified in the duplex RRT-PCR assay to monitor amplification efficiency, normalize amplification signals, and avoid false-negative results. The functionality of the GFAP mRNA RRT-PCR was assessed through analysis of laboratory-generated binary mixtures of bovine central nervous system (CNS) and muscle tissues treated under various thermal settings imitating industrial conditions. The assay was able to detect as low as 0.05 % (wt/wt) bovine brain tissue in binary mixtures heat treated at 110 to 130°C for 20 to 60 min. Further evaluation of the GFAP mRNA RRT-PCR assay involved samples of industrial rendered products of various species origin and composition obtained from commercial sources and rendering plants. Low amounts of bovine GFAP mRNA were detected in several bovine-rendered products, which was in agreement with declared species composition. An accurate estimation of CNS tissue content in industrial-rendered products was complicated due to a wide range of temperature and time settings in rendering protocols. Nevertheless, the GFAP mRNA RRT-PCR assay may be considered for bovine CNS tissue detection in rendered products in combination with other available tools (for example, animal age

  6. Effectiveness of Phytogenic Feed Additive as Alternative to Bacitracin Methylene Disalicylate on Hematological Parameters, Intestinal Histomorphology and Microbial Population and Production Performance of Japanese Quails

    PubMed Central

    Manafi, M.; Hedayati, M.; Khalaji, S.

    2016-01-01

    This study was conducted to evaluate the effects of phytogenic additive and antibiotic growth promoter in laying Japanese quails. One hundred and sixty five quails were divided into three groups of 5 replicates and 11 quails (8 females and 3 males) in each replicate. Treatment 1 was fed control diet, treatment 2 was fed control diet supplemented with 0.05% bacitracin methylene disalicylate as antibiotic growth promoter and treatment 3 was fed control diet supplemented with 0.1% phytogenic feed additive (PFA) for two periods of 3 weeks each from 37 to 42 weeks of age. Results showed that egg production, eggshell strength, eggshell weight, villus height and villus height to crypt depth ratio were significantly (p≤0.05) increased and feed consumption, feed conversion ratio, albumen, Haugh unit, cholesterol, low-density lipoprotein, alanine transaminase, gamma glutamyltransferase, alkaline phosphatase, high-density lipoprotein, triglyceride, number of goblet cell, crypt depth and intestinal bacterial population of Coliforms, Salmonella and E. coli were significantly (p≤0.05) decreased in PFA fed group. It is concluded that addition of PFA containing phytomolecules and organic acids as main ingredients could significantly improve the production parameters and the general health of laying quails as an alternative to antibiotic growth promoters. PMID:27189636

  7. Effectiveness of Phytogenic Feed Additive as Alternative to Bacitracin Methylene Disalicylate on Hematological Parameters, Intestinal Histomorphology and Microbial Population and Production Performance of Japanese Quails.

    PubMed

    Manafi, M; Hedayati, M; Khalaji, S

    2016-09-01

    This study was conducted to evaluate the effects of phytogenic additive and antibiotic growth promoter in laying Japanese quails. One hundred and sixty five quails were divided into three groups of 5 replicates and 11 quails (8 females and 3 males) in each replicate. Treatment 1 was fed control diet, treatment 2 was fed control diet supplemented with 0.05% bacitracin methylene disalicylate as antibiotic growth promoter and treatment 3 was fed control diet supplemented with 0.1% phytogenic feed additive (PFA) for two periods of 3 weeks each from 37 to 42 weeks of age. Results showed that egg production, eggshell strength, eggshell weight, villus height and villus height to crypt depth ratio were significantly (p≤0.05) increased and feed consumption, feed conversion ratio, albumen, Haugh unit, cholesterol, low-density lipoprotein, alanine transaminase, gamma glutamyltransferase, alkaline phosphatase, high-density lipoprotein, triglyceride, number of goblet cell, crypt depth and intestinal bacterial population of Coliforms, Salmonella and E. coli were significantly (p≤0.05) decreased in PFA fed group. It is concluded that addition of PFA containing phytomolecules and organic acids as main ingredients could significantly improve the production parameters and the general health of laying quails as an alternative to antibiotic growth promoters. PMID:27189636

  8. A comparative analysis of well-to-wheel primary energy demand and greenhouse gas emissions for the operation of alternative and conventional vehicles in Switzerland, considering various energy carrier production pathways

    NASA Astrophysics Data System (ADS)

    Yazdanie, Mashael; Noembrini, Fabrizio; Dossetto, Lionel; Boulouchos, Konstantinos

    2014-03-01

    This study provides a comprehensive analysis of well-to-wheel (WTW) primary energy demand and greenhouse gas (GHG) emissions for the operation of conventional and alternative passenger vehicle drivetrains. Results are determined based on a reference vehicle, drivetrain/production process efficiencies, and lifecycle inventory data specific to Switzerland. WTW performance is compared to a gasoline internal combustion engine vehicle (ICEV). Both industrialized and novel hydrogen and electricity production pathways are evaluated. A strong case is presented for pluggable electric vehicles (PEVs) due to their high drivetrain efficiency. However, WTW performance strongly depends on the electricity source. A critical electricity mix can be identified which divides optimal drivetrain performance between the EV, ICEV, and plug-in hybrid vehicle. Alternative drivetrain and energy carrier production pathways are also compared by natural resource. Fuel cell vehicle (FCV) performance proves to be on par with PEVs for energy carrier (EC) production via biomass and natural gas resources. However, PEVs outperform FCVs via solar energy EC production pathways. ICE drivetrains using alternative fuels, particularly biogas and CNG, yield remarkable WTW energy and emission reductions as well, indicating that alternative fuels, and not only alternative drivetrains, play an important role in the transition towards low-emission vehicles in Switzerland.

  9. Alternative Schools.

    ERIC Educational Resources Information Center

    Pritchett, Stanley; Kimsey, Steve

    2002-01-01

    Describes the design of the DeKalb Alternative School in Atlanta, Georgia, located in a renovated shopping center. Purchasing commercial land and renovating the existing building saved the school system time and money. (EV)

  10. Monitoring Regional Forest Disturbances across the US with Near Real Time MODIS NDVI Products included in the ForWarn Forest Threat Early Warning System

    NASA Astrophysics Data System (ADS)

    Spruce, J.; Hargrove, W. W.; Gasser, J.; Norman, S. P.

    2013-12-01

    Forest threats across the US have become increasingly evident in recent years. These include regionally extensive disturbances (e.g., from drought, bark beetle outbreaks, and wildfires) that can occur across multiyear durations and result in extensive forest mortality. In addition, forests can be subject to ephemeral, sometimes yearly defoliation from various insects and types of storm damage. After prolonged severe disturbance, signs of forest recovery can vary in terms of satellite-based Normalized Difference Vegetation Index (NDVI) values. The increased extent and threat of forest disturbances in part led to the enactment of the 2003 Healthy Forest Restoration Act, which mandated that a national forest threat Early Warning System (EWS) be deployed. In response, the US Forest Service collaborated with NASA, DOE Oak Ridge National Laboratory, and the USGS Eros Data Center to build the near real time ForWarn forest threat EWS for monitoring regionally evident forest disturbances, starting on-line operations in 2010. Given the diversity of disturbance types, severities, and durations, ForWarn employs multiple historical baselines used with current NDVI to derive a suite of six nationwide 'weekly' forest change products. ForWarn uses daily 232 meter MODIS Aqua and Terra satellite NDVI data, including MOD13 products for deriving historical baseline NDVIs and eMODIS products for compiling current NDVI. Separately pre-processing the current and historical NDVIs, the Time Series Product Tool and the Phenological Parameters Estimation Tool are used to temporally reduce noise, fuse, and aggregate MODIS NDVIs into 24 day composites refreshed every 8 days with 46 dates of forest change products per year. The 24 day compositing interval typically enables new disturbances to be detected, while minimizing the frequency of residual atmospheric contamination. ForWarn's three standard forest change products compare current NDVI to that from the previous year, previous 3 years, and

  11. Real Forestry for Real Estate

    ERIC Educational Resources Information Center

    Gagnon, Jennifer; Fisher, Jason

    2013-01-01

    Virginia is poised to see an unprecedented change in forest land ownership. To provide new landowners with information on sustainable forest management, we developed a two-part program, Real Forestry for Real Estate. First, we assembled New Landowner Packets, which contain a variety of sustainable forest management resources. Second, two…

  12. Online Measurement of Real-Time Cytotoxic Responses Induced by Multi-Component Matrices, such as Natural Products, through Electric Cell-Substrate Impedance Sensing (ECIS)

    PubMed Central

    Fallarero, Adyary; Batista-González, Ana E.; Hiltunen, Anna K.; Liimatainen, Jaana; Karonen, Maarit; Vuorela, Pia M.

    2015-01-01

    Natural products are complex matrices of compounds that are prone to interfere with the label-dependent methods that are typically used for cytotoxicity screenings. Here, we developed a label-free Electric Cell-substrate Impedance Sensing (ECIS)-based cytotoxicity assay that can be applied in the assessment of the cytotoxicity of natural extracts. The conditions to measure the impedance using ECIS were first optimized in mice immortalized hypothalamic neurons GT1-7 cells. The performance of four natural extracts when tested using three conventional cytotoxicity assays in GT1-7 cells, was studied. Betula pendula (silver birch tree) was found to interfere with all of the cytotoxicity assays in which labels were applied. The silver birch extract was also proven to be cytotoxic and, thus, served as a proof-of-concept for the use of ECIS. The extract was fractionated and the ECIS method permitted the distinction of specific kinetic patterns of cytotoxicity on the fractions as well as the extract’s pure constituents. This study offers evidence that ECIS is an excellent tool for real-time monitoring of the cytotoxicity of complex extracts that are difficult to work with using conventional (label-based) assays. Altogether, it offers a very suitable cytotoxicity-screening assay making the work with natural products less challenging within the drug discovery workflow. PMID:26569236

  13. Evaluating the co-production of a near real time Earthquake Aftershock forecasting tool for humanitarian risk assessment and emergency planning

    NASA Astrophysics Data System (ADS)

    Quinn, Keira; Hope, Max; McCloskey, John; NicBhloscaidh, Mairead; Jimenez, Abigail; Dunlop, Paul

    2015-04-01

    Concern Worldwide and the University of Ulster Geophysics Research Group are engaged in a project to co-produce a suite of software and mapping tools to assess aftershock hazard in near real-time during the emergency response phase of earthquake disaster, and inform humanitarian emergency planning and response activities. This paper uses a social learning approach to evaluate this co-production process. Following Wenger (1999) we differentiate between the earthquake science and humanitarian communities of practice (CoP) along three dimensions: enterprise (the purpose of CoPs and the problems participants are working to address), repertoire (knowledge, skills, language), and identity (values and boundaries). We examine the effectiveness of learning between CoP, focusing on boundary work and objects, and various organisational structures and aspects of the wider political economy of learning that enable and hinder the co-production process. We conclude by identifying a number of ways to more effectively integrate earthquake science into humanitarian decision-making, policy development and programme design.

  14. Online Measurement of Real-Time Cytotoxic Responses Induced by Multi-Component Matrices, such as Natural Products, through Electric Cell-Substrate Impedance Sensing (ECIS).

    PubMed

    Fallarero, Adyary; Batista-González, Ana E; Hiltunen, Anna K; Liimatainen, Jaana; Karonen, Maarit; Vuorela, Pia M

    2015-01-01

    Natural products are complex matrices of compounds that are prone to interfere with the label-dependent methods that are typically used for cytotoxicity screenings. Here, we developed a label-free Electric Cell-substrate Impedance Sensing (ECIS)-based cytotoxicity assay that can be applied in the assessment of the cytotoxicity of natural extracts. The conditions to measure the impedance using ECIS were first optimized in mice immortalized hypothalamic neurons GT1-7 cells. The performance of four natural extracts when tested using three conventional cytotoxicity assays in GT1-7 cells, was studied. Betula pendula (silver birch tree) was found to interfere with all of the cytotoxicity assays in which labels were applied. The silver birch extract was also proven to be cytotoxic and, thus, served as a proof-of-concept for the use of ECIS. The extract was fractionated and the ECIS method permitted the distinction of specific kinetic patterns of cytotoxicity on the fractions as well as the extract's pure constituents. This study offers evidence that ECIS is an excellent tool for real-time monitoring of the cytotoxicity of complex extracts that are difficult to work with using conventional (label-based) assays. Altogether, it offers a very suitable cytotoxicity-screening assay making the work with natural products less challenging within the drug discovery workflow. PMID:26569236

  15. Innovative radar products for the 3D, high-resolution and real-time monitoring of the convective activity in the airspace around airports

    NASA Astrophysics Data System (ADS)

    Tabary, P.; Bousquet, O.; Sénési, S.; Josse, P.

    2009-09-01

    Airports are recognized to become critical areas in the future given the expected doubling in air traffic by 2020. The increased density of aircrafts in the airport airspaces calls for improved systems and products to monitor in real-time potential hazards and thus meet the airport objectives in terms of safety and throughput. Among all meteorological hazards, convection is certainly the most impacting one. We describe here some innovative radar products that have recently been developed and tested at Météo France around the Paris airports. Those products rely on the French Doppler radar network consisting today of 24 elements with some of them being polarimetric. Reflectivity and Doppler volumetric data are concentrated from all 24 radar sites in real-time at the central level (Toulouse) where 3D Cartesian mosaics covering the entire French territory (i.e. a typical 1,000 by 1,000 km² area) are elaborated. The innovation with respect to what has been done previously is that the three components of the wind are retrieved by operational combination of the radial velocities. The final product, available in real-time every 15 minutes with a spatial resolution of 2.5 km horizontally and 500 m vertically, is a 3D grid giving the interpolated reflectivity and wind field (u, v and w) values. The 2.5 km resolution, arising from the fact that the retrieval is carried out every 15 minutes from radars typically spaced apart by 150 km, is not sufficient for airport airspace monitoring but is valuable for en-route monitoring. Its extension to the entire European space is foreseen. To address the specific needs in the airport areas, a downscaling technique has been proposed to merge the above-mentioned low-resolution 3D wind and reflectivity fields with the high resolution (5 minutes and 1 km²) 2D imagery of the Trappes radar that is the one that covers the Paris airports. The merging approach is based on the assumption that the Vertical Profile of Reflectivity (i.e. the

  16. Monitoring 2009 Forest Disturbance across the Conterminous United States, Based on Near-Real Time and Historical MODIS 250 Meter NDVI Products

    NASA Astrophysics Data System (ADS)

    Spruce, J.; Hargrove, W. W.; Gasser, G.; Smoot, J. C.; Kuper, P.

    2009-12-01

    This presentation discusses a study on the use of MODIS NDVI data for viewing regional patterns of forest disturbance across the conterminous United States. This capability is a part of a national forest threat early warning system (EWS) being developed by the USDA Forest Service’s Eastern and Western Environmental Threat Centers with help from NASA Stennis Space Center and the Oak Ridge National Laboratory. The viewing capability of the EWS was recently demonstrated for 2009, using near-real time (NRT) MODIS NDVI data from the USGS eMODIS Web site and historical NDVI data from standard MOD13 products. For this study, a historical maximum NDVI baseline for CONUS was computed from fused Aqua and Terra MOD13 data for June 10-July 27 of each year during 2000-2006. Comparable 2009 MODIS NDVI imagery was computed from fusion and re-compositing of eMODIS NRT Aqua and Terra 7-day products. For the historical data, time series data processing software was used to remove poor quality data and to mitigate data gaps mainly due to clouds. Although the NRT component was not as rigorously processed to mitigate noise, the processing still yielded largely cloud-free clean, coherent CONUS NDVI imagery initially with only 21-days of compositing. The principal end product of the study was a forest disturbance visualization product based on an NDVI RGB image that combines data from 2 dates (i.e. time frames). For this RGB, the historical maximum NDVI for the observed temporal window was assigned to the red color gun and the 2009 NRT product for the same time frame was assigned to the blue and green guns. The resulting image was masked with a USFS FIA 250-m type map to include only forested areas. The forest disturbance areas on the forest-masked 2-date NDVI RGB are shown in red tones with non-disturbed closed canopy forest generally shown in medium to bright gray tones. This product highlighted several broad-scaled forest canopy disturbances for the observed time in 2009, including

  17. Consequences of the ban of by-products from terrestrial animals in livestock feeding in Germany and the European Union: alternatives, nutrient and energy cycles, plant production, and economic aspects.

    PubMed

    Rodehutscord, M; Abel, H J; Friedt, W; Wenk, C; Flachowsky, G; Ahlgrimm, H J; Johnke, B; Kühl, R; Breves, G

    2002-04-01

    Consequences of the ban of meat and bone meal (MBM) and animal fat with regard to livestock feeding, cropping, ecology and economy where investigated with an inter-disciplinary approach for Germany and the European Union. Calculations were made for different production systems with pigs and poultry on the basis of statistical data for the production and for the feed markets as well as from requirement data for the respective species and production system. (1.) The ban of MBM from feeding caused a need for alternative protein sources. If all the amount of protein from MBM is to be replaced by soybean meal, in Germany and the EU about 0.30 and 2.30 x 10(6) t would be needed each year (supplementary amino acids not considered). Alternatively, doubling the grain legume acreage in Germany to about 420,000 ha would supply a similar amount of protein. A wider application of phase feeding with adjusted dietary amino acid concentrations, however, would allow for saving protein to an extent which is similar to the amount of protein that was contributed by MBM in recent years. Thus, the ban is a minor problem in terms of ensuring amino acid supply. (2.) However, alternative plant ingredients cannot compensate for the gap in P supply that is caused by the ban. An additional demand for inorganic feed phosphates of about 14,000 and 110,000 t per year is given in Germany and the EU, respectively. So far, this gap is filled almost completely by increased mining of rock phosphates. Alternatively, a general application of microbial phytase to all diets would largely fill this gap. Until the ban, MBM contributed to 57% of the supplementation of P that was needed for pigs and poultry. The ban of MBM makes large amounts of P irreversibly disappearing from the food chain. (3.) Energy from slaughter offal and cadavers can be utilized in different technologies, in the course of which the efficiency of energy utilisation depends on the technology applied. It is efficient in the cement work

  18. Alternative Geothermal Power Production Scenarios

    DOE Data Explorer

    Sullivan, John

    2014-03-14

    The information given in this file pertains to Argonne LCAs of the plant cycle stage for a set of ten new geothermal scenario pairs, each comprised of a reference and improved case. These analyses were conducted to compare environmental performances among the scenarios and cases. The types of plants evaluated are hydrothermal binary and flash and Enhanced Geothermal Systems (EGS) binary and flash plants. Each scenario pair was developed by the LCOE group using GETEM as a way to identify plant operational and resource combinations that could reduce geothermal power plant LCOE values. Based on the specified plant and well field characteristics (plant type, capacity, capacity factor and lifetime, and well numbers and depths) for each case of each pair, Argonne generated a corresponding set of material to power ratios (MPRs) and greenhouse gas and fossil energy ratios.

  19. Environmentally conscious alternative energy production

    SciTech Connect

    Kutz, M.

    2007-09-15

    This fourth volume of the series describes and compares the environmental and economic impacts of renewable and conventional power generation technologies. Chapter heading are: Economic comparisons of power generation technologies (Todd Nemec); Solar energy applications (Jan F. Kreider); Fuel cells (Matthew W. Mench); Geothermal resources and technology: an introduction (Peter D. Blair); Wind power generation (Todd Nemec); Cogeneration (Jerald Caton); Hydrogen energy (Elias K. Stefanakos, Yogi Goswami, S.S. Srinivasan, and J.T. Wolan); Clean power generation from coal (Prabir Basu and James Butler); and Using waste heat from power plants (Herbert A. Ingley). The chapter on clean coal power generation from coal has been abstracted separately on the Coal Abstracts database. 2 apps.

  20. Monitoring Regional Forest Disturbances across the US with near Real Time MODIS NDVI Products Resident to the ForWarn Forest Threat Early Warning System

    NASA Technical Reports Server (NTRS)

    Spruce, Joseph P.; Hargrove, William W.; Gasser, Gerald

    2013-01-01

    Forest threats across the US have become increasingly evident in recent years. Sometimes these have resulted in regionally evident disturbance progressions (e.g., from drought, bark beetle outbreaks, and wildfires) that can occur across multiyear durations and have resulted in extensive forest overstory mortality. In addition to stand replacement disturbances, other forests are subject to ephemeral, sometimes yearly defoliation from various insects and varying types and intensities of ephemeral damage from storms. Sometimes, after prolonged severe disturbance, signs of recovery in terms of Normalized Difference Vegetation Index (NDVI) can occur. The growing prominence and threat of forest disturbances in part have led to the formation and implementation of the 2003 Healthy Forest Restoration Act which mandated that national forest threat early warning system be developed and deployed. In response, the US Forest Service collaborated with NASA, DOE Oakridge National Laboratory, and the USGS Eros Data Center to build and roll-out the near real time ForWarn early warning system for monitoring regionally evident forest disturbances. Given the diversity of disturbance types, severities, and durations, ForWarn employs multiple historical baselines that are used with current NDVI to derive a suite of six forest change products that are refreshed every 8 days. ForWarn employs daily quarter kilometer MODIS NDVI data from the Aqua and Terra satellites, including MOD13 data for deriving historical baseline NDVIs and eMODIS 7 NDVI for compiling current NDVI. In doing so, the Time Series Product Tool and the Phenological Parameters Estimation Tool are used to temporally de-noise, fuse, and aggregate current and historical MODIS NDVIs into 24 day composites refreshed every 8 days with 46 dates of products per year. The 24 day compositing interval enables disturbances to be detected, while minimizing the frequency of residual atmospheric contamination. Forest change products are