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1

Investigation of effect of variations in bone fraction and red marrow cellularity on bone marrow dosimetry in radio-immunotherapy  

PubMed Central

A method is described for computing patient-specific absorbed dose-rates to active marrow which accounts for spatial variation in bone volume fraction and marrow cellularity. A module has been added to the 3D Monte Carlo dosimetry program DPM to treat energy deposition in the components of bone spongiosa distinctly. Homogeneous voxels in regions containing bone spongiosa (as defined on CT images) are assumed to be comprised only of bone, active (red) marrow and inactive (yellow) marrow. Cellularities are determined from biopsy, and bone volume fractions are computed from cellularities and CT-derived voxel densities. Electrons are assumed to deposit energy locally in the three constituent components in proportions determined by electron energy absorption fractions which depend on energy, cellularity, and bone volume fraction, and which are either taken from the literature or are derived from Monte Carlo simulations using EGS5. Separate algorithms are used to model primary ? particles and secondary electrons generated after photon interactions. Results: Treating energy deposition distinctly in bone spongiosa constituents leads to marrow dosimetry results which differ from homogeneous spongiosa dosimetry by up to 20%. Dose rates in active marrow regions with cellularities of 20, 50, and 80% can vary by up to 20%, and can differ by up to 10% as a function of bone volume fraction. Conclusions: Dose to bone marrow exhibits a strong dependence on marrow cellularity and a potentially significant dependence on bone volume fraction. PMID:23780474

Wilderman, S J; Roberson, P L; Bolch, W E; Dewaraja, Y K

2013-01-01

2

Hyperemic peripheral red marrow in a patient with sickle cell anemia demonstrated on Tc-99m labeled red blood cell venography  

SciTech Connect

A 25-year-old gravid woman, homozygous for sickle cell anemia, with a history of recent deep venous thrombosis, was examined using Tc-99m labeled red blood cell venography for recurrent thrombosis. Although negative for thrombus, the study presented an unusual incidental finding: the patient's peripheral bone marrow was hyperemic in a distribution consistent with peripheral red bone marrow expansion. Such a pattern has not been documented before using this technique. This report supports other literature that has demonstrated hyperemia of peripheral red bone marrow in other hemolytic anemias. This finding may ultimately define an additional role of scintigraphy in assessing the pathophysiologic status of the sickle cell patient.

Heiden, R.A.; Locko, R.C.; Stent, T.R. (Columbia Univ. College of Physicians and Surgeons, New York, NY (USA))

1991-03-01

3

Exposure of red bone marrow to ionising radiation from natural and medical sources in France.  

PubMed

This paper presents a review of available data on the average level of equivalent dose to the red bone marrow in France, due to natural and some medical exposures. The sources of exposure to ionising radiation which were studied are medical examinations involving x-rays (diagnostic radiology), inhalation of radon and thoron, cosmic irradiation, terrestrial irradiation and intakes of natural radionuclides. This review is based, where possible, on specific data for France. The influence of age on exposure levels is studied when data are available. The total equivalent dose to the red bone marrow is estimated at 2.4 mSv year(-1), 2.7 mSv year(-1) and 5.4 mSv year(-1) respectively for adults (> or =18 years old), children (3-17 years old) and infants (0-1 year old). The high level of exposure for infants is explained by the important contribution of 210Po. The average equivalent dose to the red bone marrow for adults is also compared with the average committed effective dose due to the same exposure sources. The importance of medical exposure and natural exposure due to intake of natural radionuclides emphasises the importance of obtaining more information about these sources of exposure. PMID:11594649

Rommens, C; Ringeard, C; Hubert, P

2001-09-01

4

660 nm red light-enhanced bone marrow mesenchymal stem cell transplantation for hypoxic-ischemic brain damage treatment  

PubMed Central

Bone marrow mesenchymal stem cell transplantation is an effective treatment for neonatal hypoxic-ischemic brain damage. However, the in vivo transplantation effects are poor and their survival, colonization and differentiation efficiencies are relatively low. Red or near-infrared light from 600–1,000 nm promotes cellular migration and prevents apoptosis. Thus, we hypothesized that the combination of red light with bone marrow mesenchymal stem cell transplantation would be effective for the treatment of hypoxic-ischemic brain damage. In this study, the migration and colonization of cultured bone marrow mesenchymal stem cells on primary neurons after oxygen-glucose deprivation were detected using Transwell assay. The results showed that, after a 40-hour irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2, an increasing number of green fluorescence-labeled bone marrow mesenchymal stem cells migrated towards hypoxic-ischemic damaged primary neurons. Meanwhile, neonatal rats with hypoxic-ischemic brain damage were given an intraperitoneal injection of 1 × 106 bone marrow mesenchymal stem cells, followed by irradiation under red light-emitting diodes at 660 nm and 60 mW/cm2 for 7 successive days. Shuttle box test results showed that, after phototherapy and bone marrow mesenchymal stem cell transplantation, the active avoidance response rate of hypoxic-ischemic brain damage rats was significantly increased, which was higher than that after bone marrow mesenchymal stem cell transplantation alone. Experimental findings indicate that 660 nm red light emitting diode irradiation promotes the migration of bone marrow mesenchymal stem cells, thereby enhancing the contribution of cell transplantation in the treatment of hypoxic-ischemic brain damage. PMID:25206807

Li, Xianchao; Hou, Wensheng; Wu, Xiaoying; Jiang, Wei; Chen, Haiyan; Xiao, Nong; Zhou, Ping

2014-01-01

5

Phagocytosis of Colloidal Carbon and Heterologous Red Blood Cells in the Bone Marrow of Rats and Rabbits  

PubMed Central

Phagocytosis in the bone marrow of rats and rabbits was studied following intravenous injections of colloidal carbon and chicken red blood cells. In both animal species the marrow response to these different foreign particles was quite similar. There was an initial aggregation and degranulation of platelets around the injected particulate matter within the marrow sinuses. Then pseudopods of marrow macrophages appeared in the sinus lumen, forming a web-like structure which trapped and phagocytosed the injected foreign material as well as the platelets. Within the phagosomes of these macrophages, the injected material and platelets were withdrawn into the parenchyma, where degradation or storage occurred. This sequence of events suggests that platelets may play an important role in marrow phagocytosis. The most active cells in marrow phagocytosis are the macrophages. The endothelial cells participated in the phagocytosis of colloidal carbon. The amount of carbon within these cells, however, was small in comparison with that trapped by macrophages. Further, the endothelial cells did not phagocytose chicken red blood cells. These results, being similar to those obtained in lymph, spleen and liver, challenge the concept of the reticuloendothelial system. The term of macrophage system is proposed as a replacement. ImagesFig 1Fig 5Fig 6Fig 7Fig 2Fig 8Fig 9Fig 3Fig 10Fig 4 PMID:4432913

Luk, S. C.; Simon, G. T.

1974-01-01

6

Influence of Age, Sex and Calendar Year on Lifetime Accumulated Red Bone Marrow Dose from Diagnostic Radiation Exposure  

PubMed Central

Our aim is to evaluate the relevance of different factors influencing lifetime accumulated red bone marrow dose, such as calendar year, age and sex. The lifetime dose was estimated for controls interviewed in person (N?=?2811, 37.5% women) of the population-based representative Northern Germany Leukemia and Lymphoma Study. Data were assessed in standardized computer-assisted personal interviews. The calculation of doses is based on a comprehensive quantification model including calendar year, sex, kind of examination, and technical development. In multivariate regression models the annual red bone marrow dose was analyzed depending on age, sex and calendar year to consider simultaneously temporal changes in radiologic practice and individual risk factors. While the number of examinations continuously rises over time, the dose shows two peaks around 1950 and after 1980. Men are exposed to higher doses than woman. Until 1970 traditional examinations like conventional and mass screening examinations caused the main dose. They were then replaced by technically advanced examinations mainly computed tomography and cardiac catheter. The distribution of the red bone marrow dose over lifetime depends highly on the technical standards and radiation protection survey. To a lesser extent it is influenced by age and sex of the subjects. Thus epidemiological studies concerning the assessment of radiation exposure should consider the calendar year in which the examination was conducted. PMID:24244286

Weitmann, Kerstin; Terschüren, Claudia; von Boetticher, Heiner

2013-01-01

7

Influence of age, sex and calendar year on lifetime accumulated red bone marrow dose from diagnostic radiation exposure.  

PubMed

Our aim is to evaluate the relevance of different factors influencing lifetime accumulated red bone marrow dose, such as calendar year, age and sex. The lifetime dose was estimated for controls interviewed in person (N?=?2811, 37.5% women) of the population-based representative Northern Germany Leukemia and Lymphoma Study. Data were assessed in standardized computer-assisted personal interviews. The calculation of doses is based on a comprehensive quantification model including calendar year, sex, kind of examination, and technical development. In multivariate regression models the annual red bone marrow dose was analyzed depending on age, sex and calendar year to consider simultaneously temporal changes in radiologic practice and individual risk factors. While the number of examinations continuously rises over time, the dose shows two peaks around 1950 and after 1980. Men are exposed to higher doses than woman. Until 1970 traditional examinations like conventional and mass screening examinations caused the main dose. They were then replaced by technically advanced examinations mainly computed tomography and cardiac catheter. The distribution of the red bone marrow dose over lifetime depends highly on the technical standards and radiation protection survey. To a lesser extent it is influenced by age and sex of the subjects. Thus epidemiological studies concerning the assessment of radiation exposure should consider the calendar year in which the examination was conducted. PMID:24244286

Hoffmann, Wolfgang; Meiboom, Merle Friederike; Weitmann, Kerstin; Terschüren, Claudia; von Boetticher, Heiner

2013-01-01

8

Total extract of Korean red ginseng facilitates human bone marrow hematopoietic colony formation in vitro  

PubMed Central

Background The number of CD34+ cells in a peripheral blood stem cell collection is the key factor in predicting successful treatment of hematologic malignancies. Korean Red Ginseng (KRG) (Panax ginseng C.A. Meyer) is the most popular medicinal herb in Korea. The objective of this study was to determine the effect of KRG on hematopoietic colony formation. Methods Bone marrow (BM) samples were obtained from 8 human donors after acquiring informed consent. BM mononuclear cells (MNCs) were isolated, and CD34+ cells were sorted using magnetic beads. The sorted CD34+ cells were incubated with or without total extract of KRG (50 µg/mL, 100 µg/mL) or Ginsenoside Rg1 (100 µg/mL), and the hematopoietic colony assay was performed using methylcellulose semisolid medium. The CD34+ cell counts were measured by a single platform assay using flow cytometry. Results The numbers of human BM-MNCs and CD34+ cells obtained after purification were variable among donors (5.6×107 and 1.3-48×107 and 8.9×104 and 1.8-80×104, respectively). The cells expanded 1,944 times after incubation for 12 d. Total extract of KRG added to the hematopoietic stem cell (HSC)-specific medium increased CD34+ cell counts 3.6 times compared to 2.6 times when using HSC medium alone. Total numbers of hematopoietic colonies in KRG medium were more than those observed in conventional medium, especially that of erythroid colonies such as burst forming unit-erythroid. Conclusion Total extract of KRG facilitated CD34+ cell expansion and hematopoietic colony formation, especially of the erythroid lineage. PMID:25325037

Kim, Sang-Gyung; Bae, Sung Hwa; Kim, Seong-Mo; Lee, Ji-Hye; Kim, Min Ji; Jang, Hae-Bong

2014-01-01

9

Fluorescence microscopy is superior to polarized microscopy for detecting amyloid deposits in Congo red-stained trephine bone marrow biopsy specimens.  

PubMed

The classic gold standard for detecting amyloid deposits is Congo red-stained bright field and polarized microscopy (CRPM). A prior study showed that Congo red fluorescence (CRF) microscopy had increased sensitivity compared with traditional CRPM when analyzing fat pad specimens. The purpose of the current study was to determine the sensitivity of CRF for evaluating Congo red-stained bone marrow biopsy specimens, and to compare these results with those of CRPM. We compared the CRPM and the CRF analyses of 33 trephine bone marrow biopsy specimens with clinical or morphologic suspicion of amyloid deposits. These results were verified against immunohistochemical staining with anti-amyloid P antibody. CRF achieved 100% sensitivity, and CRPM achieved 75% sensitivity. Both groups showed 100% specificity compared with amyloid P immunohistochemical staining. The results show that CRF is a sensitive method to analyze trephine bone marrow biopsy specimens for amyloid deposits. PMID:23010714

Marcus, Alan; Sadimin, Evita; Richardson, Maurice; Goodell, Lauri; Fyfe, Billie

2012-10-01

10

Differentiation between Focal Malignant Marrow-Replacing Lesions and Benign Red Marrow Deposition of the Spine with T2*-Corrected Fat-Signal Fraction Map Using a Three-Echo Volume Interpolated Breath-Hold Gradient Echo Dixon Sequence  

PubMed Central

Objective To assess the feasibility of T2*-corrected fat-signal fraction (FF) map by using the three-echo volume interpolated breath-hold gradient echo (VIBE) Dixon sequence to differentiate between malignant marrow-replacing lesions and benign red marrow deposition of vertebrae. Materials and Methods We assessed 32 lesions from 32 patients who underwent magnetic resonance imaging after being referred for assessment of a known or possible vertebral marrow abnormality. The lesions were divided into 21 malignant marrow-replacing lesions and 11 benign red marrow depositions. Three sequences for the parameter measurements were obtained by using a 1.5-T MR imaging scanner as follows: three-echo VIBE Dixon sequence for FF; conventional T1-weighted imaging for the lesion-disc ratio (LDR); pre- and post-gadolinium enhanced fat-suppressed T1-weighted images for the contrast-enhancement ratio (CER). A region of interest was drawn for each lesion for parameter measurements. The areas under the curve (AUC) of the parameters and their sensitivities and specificities at the most ideal cutoff values from receiver operating characteristic curve analysis were obtained. AUC, sensitivity, and specificity were respectively compared between FF and CER. Results The AUCs of FF, LDR, and CER were 0.96, 0.80, and 0.72, respectively. In the comparison of diagnostic performance between the FF and CER, the FF showed a significantly larger AUC as compared to the CER (p = 0.030), although the difference of sensitivity (p = 0.157) and specificity (p = 0.157) were not significant. Conclusion Fat-signal fraction measurement using T2*-corrected three-echo VIBE Dixon sequence is feasible and has a more accurate diagnostic performance, than the CER, in distinguishing benign red marrow deposition from malignant bone marrow-replacing lesions. PMID:25469090

Kim, Yong Pyo; Kannengiesser, Stephan; Paek, Mun-Young; Chung, Tae-Sub; Yoo, Yeon Hwa; Yoon, Choon-Sik; Song, Ho-Taek; Lee, Young Han; Suh, Jin-Suck

2014-01-01

11

Evaluation of dual energy quantitative CT for determining the spatial distributions of red marrow and bone for dosimetry in internal emitter radiation therapy  

SciTech Connect

Purpose: To evaluate a three-equation three-unknown dual-energy quantitative CT (DEQCT) technique for determining region specific variations in bone spongiosa composition for improved red marrow dose estimation in radionuclide therapy. Methods: The DEQCT method was applied to 80/140 kVp images of patient-simulating lumbar sectional body phantoms of three sizes (small, medium, and large). External calibration rods of bone, red marrow, and fat-simulating materials were placed beneath the body phantoms. Similar internal calibration inserts were placed at vertebral locations within the body phantoms. Six test inserts of known volume fractions of bone, fat, and red marrow were also scanned. External-to-internal calibration correction factors were derived. The effects of body phantom size, radiation dose, spongiosa region segmentation granularity [single (?17 × 17 mm) region of interest (ROI), 2 × 2, and 3 × 3 segmentation of that single ROI], and calibration method on the accuracy of the calculated volume fractions of red marrow (cellularity) and trabecular bone were evaluated. Results: For standard low dose DEQCT x-ray technique factors and the internal calibration method, the RMS errors of the estimated volume fractions of red marrow of the test inserts were 1.2–1.3 times greater in the medium body than in the small body phantom and 1.3–1.5 times greater in the large body than in the small body phantom. RMS errors of the calculated volume fractions of red marrow within 2 × 2 segmented subregions of the ROIs were 1.6–1.9 times greater than for no segmentation, and RMS errors for 3 × 3 segmented subregions were 2.3–2.7 times greater than those for no segmentation. Increasing the dose by a factor of 2 reduced the RMS errors of all constituent volume fractions by an average factor of 1.40 ± 0.29 for all segmentation schemes and body phantom sizes; increasing the dose by a factor of 4 reduced those RMS errors by an average factor of 1.71 ± 0.25. Results for external calibrations exhibited much larger RMS errors than size matched internal calibration. Use of an average body size external-to-internal calibration correction factor reduced the errors to closer to those for internal calibration. RMS errors of less than 30% or about 0.01 for the bone and 0.1 for the red marrow volume fractions would likely be satisfactory for human studies. Such accuracies were achieved for 3 × 3 segmentation of 5 mm slice images for: (a) internal calibration with 4 times dose for all size body phantoms, (b) internal calibration with 2 times dose for the small and medium size body phantoms, and (c) corrected external calibration with 4 times dose and all size body phantoms. Conclusions: Phantom studies are promising and demonstrate the potential to use dual energy quantitative CT to estimate the spatial distributions of red marrow and bone within the vertebral spongiosa.

Goodsitt, Mitchell M., E-mail: goodsitt@umich.edu; Shenoy, Apeksha; Howard, David; Christodoulou, Emmanuel; Dewaraja, Yuni K. [Department of Radiology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States)] [Department of Radiology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States); Shen, Jincheng [Department of Biostatistics, University of Michigan, 1415 Washington Heights, Ann Arbor, Michigan 48109 (United States)] [Department of Biostatistics, University of Michigan, 1415 Washington Heights, Ann Arbor, Michigan 48109 (United States); Schipper, Matthew J. [Department of Radiation Oncology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States)] [Department of Radiation Oncology, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States); Wilderman, Scott [Department of Nuclear Engineering, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States)] [Department of Nuclear Engineering, University of Michigan, 1500 East Medical Center Drive, Ann Arbor, Michigan 48109 (United States); Chun, Se Young [Ulsan National Institute of Science and Technology (UNIST), School of Electrical and Computer Engineering, Ulsan 689-798 (Korea, Republic of)] [Ulsan National Institute of Science and Technology (UNIST), School of Electrical and Computer Engineering, Ulsan 689-798 (Korea, Republic of)

2014-05-15

12

Comparison of mathematical models for red marrow and blood absorbed dose estimation in the radioiodine treatment of advanced differentiated thyroid carcinoma  

NASA Astrophysics Data System (ADS)

Metastatic and recurrent differentiated thyroid carcinoma is preferably treated with 131I, whose administered activity is limited by red marrow (RM) toxicity, originally correlated by Benua to a blood absorbed dose higher than 2?Gy. Afterward a variety of dosimetric approaches has been proposed. The aim of this work is to compare the results of the Benua formula with the ones of other three blood and RM absorbed dose formulae. Materials and methods have been borrowed by the dosimetric protocol of the Italian Internal Dosimetry group and adapted to the routine of our centre. Wilcoxon t-tests and percentage differences have been applied for comparison purposes. Results are significantly different (p < 0.05) from each other, with an average percentage difference between Benua versus other results of ?22%. The dosimetric formula applied to determine blood or RM absorbed dose may contribute significantly to increase heterogeneity in absorbed dose and dose–response results. Standardization should be a major objective.

Miranti, A.; Giostra, A.; Richetta, E.; Gino, E.; Pellerito, R. E.; Stasi, M.

2015-02-01

13

Comparison of mathematical models for red marrow and blood absorbed dose estimation in the radioiodine treatment of advanced differentiated thyroid carcinoma.  

PubMed

Metastatic and recurrent differentiated thyroid carcinoma is preferably treated with (131)I, whose administered activity is limited by red marrow (RM) toxicity, originally correlated by Benua to a blood absorbed dose higher than 2?Gy. Afterward a variety of dosimetric approaches has been proposed. The aim of this work is to compare the results of the Benua formula with the ones of other three blood and RM absorbed dose formulae. Materials and methods have been borrowed by the dosimetric protocol of the Italian Internal Dosimetry group and adapted to the routine of our centre. Wilcoxon t-tests and percentage differences have been applied for comparison purposes. Results are significantly different (p < 0.05) from each other, with an average percentage difference between Benua versus other results of -22%. The dosimetric formula applied to determine blood or RM absorbed dose may contribute significantly to increase heterogeneity in absorbed dose and dose-response results. Standardization should be a major objective. PMID:25586549

Miranti, A; Giostra, A; Richetta, E; Gino, E; Pellerito, R E; Stasi, M

2015-02-01

14

Flow cytometric evaluation of red blood cell chimerism after bone marrow transplantation in Iranian patients: a preliminary study.  

PubMed

The aim of this study was to evaluate mixed red cells population and red blood cell chimerism after hematopoietic stem cell transplantation. Red blood cell chimerism after hematopoietic stem cell transplantation was analyzed using a series of fluorescein isothiocyanate-conjugated monoclonal antibodies (BioAtlantic, France) directed against ABH, Rh (D, C, E, c, e), Kell, Duffy, Kidd, and Ss antigens on blood samples of 14 patients with hematologic disorders undergoing hematopoietic stem cell transplantation, by flow cytometric method on days 15, 30, and 60 after transplantation. All patients showed expression of donor red cell antigens within days 15 - 30 after hematopoietic stem cell transplantation. Graft versus host disease and ABO incompatibility did not affect the expression of chimerism. Flow cytometric analysis is a simple, accurate, and valuable test which is of significant help in monitoring chimerism in allogeneic hematopoietic stem cell transplantation. PMID:17061616

Shaiegan, Mojgan; Hadjati, Esmerdis; Aghaiipour, Mahnaz; Iravani, Masoud; David, Gaelle; Bernard, Daniel

2006-10-01

15

A METHOD FOR THE RAPID SEPARATION OF LEUKOCYTES AND NUCLEATED ERYTHROCYTES FROM BLOOD OR MARROW WITH A PHYTOHEMAGGLUTININ FROM RED BEANS (PHASEOLUS VULGARIS)  

Microsoft Academic Search

A TECHNICALLY simple and rapid method for separating living leukocytes and nucleated erythrocytes from whole blood or marrow with a high degree of efficiency, large net yield and negligible admixture with mature erythrocytes or other contaminants is needed for chemical, metabolic or cultural studies of these cells. The method presented in this paper describes a technic for accelerating the sedimentation

JONAH G. Li; EDWIN E. OSGOOD

16

Bone marrow transplant  

MedlinePLUS

Transplant - bone marrow; Stem cell transplant; Hematopoietic stem cell transplant; Reduced intensity, nonmyeloablative transplant; Mini transplant; Allogenic bone marrow transplant; Autologous bone marrow transplant; Umbilical ...

17

Bone marrow: all the cells of the immune system are derived from stem cells in the bone marrow. The bone  

E-print Network

Bone marrow: all the cells of the immune system are derived from stem cells in the bone marrow. The bone marrow is the site of origin of red blood cells, white cells (including lymphocytes and macrophages) and platelets. Thymus: in the thymus gland lymphoid cells undergo a process of maturation

Morante, Silvia

18

Use of Monte Carlo simulations with a realistic rat phantom for examining the correlation between hematopoietic system response and red marrow absorbed dose in Brown Norway rats undergoing radionuclide therapy with {sup 177}Lu- and {sup 90}Y-BR96 mAbs  

SciTech Connect

Purpose: Biokinetic and dosimetry studies in laboratory animals often precede clinical radionuclide therapies in humans. A reliable evaluation of therapeutic efficacy is essential and should be based on accurate dosimetry data from a realistic dosimetry model. The aim of this study was to develop an anatomically realistic dosimetry model for Brown Norway rats to calculate S factors for use in evaluating correlations between absorbed dose and biological effects in a preclinical therapy study. Methods: A realistic rat phantom (Roby) was used, which has some flexibility that allows for a redefinition of organ sizes. The phantom was modified to represent the anatomic geometry of a Brown Norway rat, which was used for Monte Carlo calculations of S factors. Kinetic data for radiolabeled BR96 monoclonal antibodies were used to calculate the absorbed dose. Biological data were gathered from an activity escalation study with {sup 90}Y- and {sup 177}Lu-labeled BR96 monoclonal antibodies, in which blood cell counts and bodyweight were examined up to 2 months follow-up after injection. Reductions in white blood cell and platelet counts and declines in bodyweight were quantified by four methods and compared to the calculated absorbed dose to the bone marrow or the total body. Results: A red marrow absorbed dose-dependent effect on hematological parameters was observed, which could be evaluated by a decrease in blood cell counts. The absorbed dose to the bone marrow, corresponding to the maximal tolerable activity that could safely be administered, was determined to 8.3 Gy for {sup 177}Lu and 12.5 Gy for {sup 90}Y. Conclusions: There was a clear correlation between the hematological effects, quantified with some of the studied parameters, and the calculated red marrow absorbed doses. The decline in body weight was stronger correlated to the total body absorbed dose, rather than the red marrow absorbed dose. Finally, when considering a constant activity concentration, the phantom weight, ranging from 225 g to 300 g, appeared to have no substantial effect for the estimated absorbed dose.

Larsson, Erik; Ljungberg, Michael; Martensson, Linda; Nilsson, Rune; Tennvall, Jan; Strand, Sven-Erik; Joensson, Bo-Anders [Department of Medical Radiation Physics, Clinical Sciences, Lund University, Lund (Sweden); Department of Oncology, Clinical Sciences, Lund University, Lund (Sweden); Department of Medical Radiation Physics, Clinical Sciences, Lund University, Lund (Sweden)

2012-07-15

19

Bone Marrow Tests  

MedlinePLUS

... diagnose fevers and infections. The two bone marrow tests are aspiration (as-pi-RA-shun) and biopsy. Bone marrow aspiration usually is done first. For this test, your doctor removes a small amount of fluid ...

20

Starvation marrow – gelatinous transformation of bone marrow  

PubMed Central

Gelatinous bone marrow transformation (GMT), also known as starvation marrow, represents a rare pathological entity of unclear etiology, in which bone marrow histopathology demonstrates hypoplasia, fat atrophy, and gelatinous infiltration. The finding of gelatinous marrow transformation lacks disease specificity; rather, it is an indicator of severe illness and a marker of poor nutritional status, found in patients with eating disorders, acute febrile illnesses, acquired immunodeficiency syndrome, alcoholism, malignancies, and congestive heart failure. We present a middle-aged woman with a history of alcoholism, depression, and anorexia nervosa who presented with failure to thrive and macrocytic anemia, with bone marrow examination demonstrative of gelatinous transformation, all of which resolved with appropriate treatment. To our knowledge, there are very few cases of GMT which have been successfully treated; thus, our case highlights the importance of proper supportive management. PMID:25317270

Osgood, Eric; Muddassir, Salman; Jaju, Minal; Moser, Robert; Farid, Farwa; Mewada, Nishith

2014-01-01

21

A STUDY OF PREDICTED BONE MARROW DISTRIBUTION ON CALCULATED MARROW DOSE FROM EXTERNAL RADIATION EXPOSURES USING TWO SETS OF IMAGE DATA FOR THE SAME INDIVIDUAL  

PubMed Central

Red bone marrow is among the tissues of the human body that are most sensitive to ionizing radiation, but red bone marrow cannot be distinguished from yellow bone marrow by normal radiographic means. When using a computational model of the body constructed from computed tomography (CT) images for radiation dose, assumptions must be applied to calculate the dose to the red bone marrow. This paper presents an analysis of two methods of calculating red bone marrow distribution: 1) a homogeneous mixture of red and yellow bone marrow throughout the skeleton, and 2) International Commission on Radiological Protection cellularity factors applied to each bone segment. A computational dose model was constructed from the CT image set of the Visible Human Project and compared to the VIP-Man model, which was derived from color photographs of the same individual. These two data sets for the same individual provide the unique opportunity to compare the methods applied to the CT-based model against the observed distribution of red bone marrow for that individual. The mass of red bone marrow in each bone segment was calculated using both methods. The effect of the different red bone marrow distributions was analyzed by calculating the red bone marrow dose using the EGS4 Monte Carlo code for parallel beams of monoenergetic photons over an energy range of 30 keV to 6 MeV, cylindrical (simplified CT) sources centered about the head and abdomen over an energy range of 30 keV to 1 MeV, and a whole-body electron irradiation treatment protocol for 3.9 MeV electrons. Applying the method with cellularity factors improves the average difference in the estimation of mass in each bone segment as compared to the mass in VIP-Man by 45% over the homogenous mixture method. Red bone marrow doses calculated by the two methods are similar for parallel photon beams at high energy (above about 200 keV), but differ by as much as 40% at lower energies. The calculated red bone marrow doses differ significantly for simplified CT and electron beam irradiation, since the computed red bone marrow dose is a strong function of the cellularity factor applied to bone segments within the primary radiation beam. These results demonstrate the importance of properly applying realistic cellularity factors to computation dose models of the human body. PMID:19430219

Caracappa, Peter F.; Chao, T. C. Ephraim; Xu, X. George

2010-01-01

22

THE BONE MARROW IX PREGNANCY AND THE PUERPERIUM  

Microsoft Academic Search

With the technical assistance of MADum.EINE LALONDE IN THIS PA1?ER morphologic studies of the hone marrow in uiormal pregnancy and the puerperium are compared w'ith the bone marrow findings in normal nomi-pregnant women of childbearing age. ? These studies were initiated for the following reasons: 1. Previous studies have show-ui that there is an increase of the total circulating red

LOUIS LOWENSTEIN; CATHERINA A. BRAMLAGE; MADELEINE LALONDE

1957-01-01

23

Magnetic resonance imaging of the normal bone marrow  

Microsoft Academic Search

The bone marrow is a complex organ that contains fat and nonfat cells, the proportions of which vary greatly with age and\\u000a in the different bones of the skeleton. Magnetic resonance (MR) imaging provides information on the composition of the medullary\\u000a cavity of any given bone and on the distribution of red and yellow marrow in the skeleton. This article

Bruno C. Vande Berg; Jacques Malghem; Frederic E. Lecouvet; Baudouin Maldague

1998-01-01

24

Serum ferritin assay and bone-marrow iron stores in patients on maintenance hemodialysis  

Microsoft Academic Search

Serum ferritin assay and bone-marrow iron stores in maintenance hemodialysis patients. Fifty-five patients on maintenance hemodialysis underwent bone marrow aspirations for evaluation of iron stores that were to be compared to concomitant measurements of hematocrit, red blood cell volume, serum iron concentration, total iron binding capacity, transferrin saturation, and serum ferritin concentration. In 42 patients (76.4%), the bone-marrow iron stores

John D Bell; William R Kincaid; Richard G Morgan; Harvey Bunce; Jack B Alperin; Harry E Sarles; August R Remmers

1980-01-01

25

Bone marrow edema syndrome  

Microsoft Academic Search

Bone marrow edema syndrome (BMES) refers to transient clinical conditions with unknown pathogenic mechanism, such as transient\\u000a osteoporosis of the hip (TOH), regional migratory osteoporosis (RMO), and reflex sympathetic dystrophy (RSD). BMES is primarily\\u000a characterized by bone marrow edema (BME) pattern. The disease mainly affects the hip, the knee, and the ankle of middle-aged\\u000a males. Many hypotheses have been proposed

Anastasios V. Korompilias; Apostolos H. Karantanas; Marios G. Lykissas; Alexandros E. Beris

2009-01-01

26

Marrow transplantation for leukemia  

SciTech Connect

Marrow transplantation for selected patients with leukemia, as for patients with severe combined immunologic deficiency or severe aplastic anemia, has now become an accepted clinical procedure. For patients with acute leukemia who have relapsed after achieving a remission of chemotherapy, marrow grafting from an identical twin or an HLA-identical sibling has now been demonstrated to produce median remissions as long as or longer than any reported for combination chemotherapy. In contrast to chemotherapy, marrow transplantation offers the possibility of cure for a small but significant fraction of these patients. Marrow transplantation for patients with ANL in first remission has now resulted in median survivals much longer than any reported with chemotherapy. Although it now appears that more than 50% of these patients can be cured with marrow transplantation, a much longer follow-up is indicated since some patients who achieve a complete remission with combination chemotherapy are now living for a long time, and some of these patients (less than 20%) may also be cured. Current intensive research with new modalities such as interferon, Acyclovir, Cyclosporin A, and monoclonal antibodies can reasonably be expected to improve the overall results of marrow transplantation.

Thomas, E.D.

1981-07-01

27

Aspiration and Biopsy: Bone Marrow  

MedlinePLUS

... a bone marrow sample for procedures (such as stem cell transplantation ) or other testing (such as chromosomal analysis). ... For the bone marrow aspiration, the doctor or nurse will carefully insert a needle into the biopsy ...

28

Overview of marrow transplantation  

SciTech Connect

Bone marrow transplantation is now an accepted form of therapy for many hematologic disorders including aplastic anemia, genetically determined diseases and malignant diseases, particularly leukemia, and for rescue of patients given intensive chemoradiotherapy for malignant disease. The donor may be a healthy identical twin, a family member or even an unrelated person. Selection is made on the basis of human leukocyte antigen tissue typing. Intensive chemoradiotherapy is used to suppress patients' immune systems to facilitate engraftment and destroy diseased marrow. Transfusion of platelets, erythrocytes and granulocytes (or all of these), antibiotic coverage and protection from infection are necessary during the pancytopenic period. Survival rates vary considerably depending on a patient's disease, clinical state and age. Patients with aplastic anemia transplanted early in the course of their disease have a survival rate of approximately 80%. Patients with acute lymphoblastic leukemia are usually transplanted in a second or subsequent remission and have a survival rate of 25% to 40%. Patients with acute nonlymphoblastic leukemia in remission have survivals ranging from 45% to 70%. More than 200 patients in the chronic phase of chronic granulocytic leukemia have been transplanted with survival ranging from 50% to 70%. Complications of marrow transplantation include marrow graft rejection, graft-versus-host disease, immunologic insufficiency and the possibility of recurrence of the leukemia. 14 references.

Thomas, E.D.

1985-12-01

29

Red clover  

MedlinePLUS

Red clover is a plant. The flower tops are used to make medicine. Red clover is used for many conditions, but so ... lowering cholesterol or controlling hot flashes in women. Red clover is used for cancer prevention, indigestion, high ...

30

Eye redness  

MedlinePLUS

Bloodshot eyes; Red eyes; Scleral infection; Conjunctival infection ... There are many possible causes of a red eye or eyes. Some are cause for concern; some are medical emergencies. Others are nothing to worry about. How red the eye appears ...

31

Analysis of 500 bone marrow transplants from unrelated donors (UR-BMT) facilitated by the Japan Marrow Donor Program: confirmation of UR-BMT as a standard therapy for patients with leukemia and aplastic anemia  

Microsoft Academic Search

In December 1991, the Japan Marrow Donor Program (JMDP) was established with the cooperation of the Japanese Red Cross and Japan Marrow Donor Foundation under the auspices of the Ministry of Health and Welfare in Japan. By December 1998, 122365 HLA-A,B typed volunteer marrow donors and 7207 patients had been cumulatively registered in the JMDP. The results of HLA-matching between

Y Kodera; Y Morishima; S Kato; Y Akiyama; H Sao; T Matsuyama; K Kawa; H Sakamaki; S Nakagawa; N Hirabayashi; H Dohi; S Okamoto; A Hiraoka; H Gondo; M Tsuchida; M Harada; S Asano; T Juji; T Sasazuki; F Takaku

1999-01-01

32

Effects of Spaceflight on Cells of Bone Marrow Origin  

PubMed Central

Once only a subject for science fiction novels, plans for establishing habitation on space stations, the Moon, and distant planets now appear among the short-term goals of space agencies. This article reviews studies that present biomedical issues that appear to challenge humankind for long-term spaceflights. With particularly focus on cells of bone marrow origin, studies involving changes in bone, immune, and red blood cell populations and their functions due to extended weightlessness were reviewed. Furthermore, effects of mechanical disuse on primitive stem cells that reside in the bone marrow were also included in this review. Novel biomedical solutions using space biotechnology will be required in order to achieve the goal of space exploration without compromising the functions of bone marrow, as spaceflight appears to disrupt homeostasis for all given cell types. Conflict of interest:None declared. PMID:24385745

Özçivici, Engin

2013-01-01

33

High-fidelity organic preservation of bone marrow in ca. 10 Ma amphibians  

NASA Astrophysics Data System (ADS)

Bone marrow in ca. 10 Ma frogs and salamanders from the Miocene of Libros, Spain, represents the first fossilized example of this extremely decay-prone tissue. The bone marrow, preserved in three dimensions as an organic residue, retains the original texture and red and yellow color of hematopoietic and fatty marrow, respectively; moldic osteoclasts and vascular structures are also present. We attribute exceptional preservation of the fossilized bone marrow to cryptic preservation: the bones of the amphibians formed protective microenvironments, and inhibited microbial infiltration. Specimens in which bone marrow is preserved vary in their completeness and articulation and in the extent to which the body outline is preserved as a thin film of organically preserved bacteria. Cryptic preservation of these labile tissues is thus to a large extent independent of, and cannot be predicted by, the taphonomic history of the remainder of the specimen.

McNamara, Maria E.; Orr, Patrick J.; Kearns, Stuart L.; Alcalá, Luis; Anadón, Pere; Peñalver-Mollá, Enrique

2006-08-01

34

Rhabdomyosarcoma infiltrating bone marrow.  

PubMed

We present a case of 26-year-old male, previously diagnosed as rhabdomyosarcoma (RMS) of perineal region. The peripheral smear showed a leukoerythroblastic picture with an occasional atypical cell. The bone marrow aspirate and biopsy showed monotonous sheets of malignant cells. On immunohistochemistry the tumor cells were strongly positive for desmin and negative for CD34 and CD117. This case illustrates the morphology and IHC findings in a case of RMS. Immunostains like CD34 and CD117 should be included to rule out a possibility of acute leukemia. PMID:25348638

Chauhan, Kriti; Jain, Monica; Shukla, Pragya; Grover, R K

2015-01-01

35

Corticosteroid-dependent differentiation of human marrow preadipocytes in vitro  

Microsoft Academic Search

Summary  A unique population of human bone marrow-derived, adherent fibroblastlike cells differentiates to adipocyte morphology when\\u000a grown in vitro in the presence of horse serum and hydrocortisone sodium hemisuccinate. Over the initial 8-weeks growth at\\u000a 37C, 7% CO2, these cells accumulate Oil Red O-positive lipid and form colonies of over 100 cells, which persist in confluent cultures\\u000a for over 30 weeks.

Joel S. Greenberger

1979-01-01

36

KSC CENTER DIRECTOR ACCEPTS PLAQUE FOR RECORD-SETTING BONE MARROW DONOR REGISTRATION DRIVE  

NASA Technical Reports Server (NTRS)

Kennedy Space Center's Bone Marrow Donor Registration Drive Chairman Dr. George A. Martin and Center Director Jay Honeycutt (left to right) accept a plaque from the Leukemia Society of America's Associate Executive Director Martin Bernstine and the American Red Cross' Southeast Regional Director Jeff Koenreich. Representatives from the American Red Cross and the Leukemia Society of America came to KSC to honor those involved in the record-setting Bone Marrow Donor Registration Drive held here earlier this year. Over 900 potential donors were added to the National Bone Marrow Registry as a result of the KSC drive. The drive established a new record for the most people registered in a single day for the American Red Cross in the three state region of which Florida is a part.

1996-01-01

37

Bone-marrow imaging with indium-111 chloride in aplastic anemia and myelofibrosis: concise communication  

SciTech Connect

Twenty-nine patients with aplastic anemia and 11 patients with myelofibrosis were evaluated with indium-111 chloride bone-marrow imaging, ferrokinetics, and bone-marrow core biopsies. There was good correlation between the erythrocyte cellularity of the marrow and the In-111 bone-marrow scan grades in most patients. In some, the overall scan grade tended to underestimate the erythroid elements because the core biopsy had been taken from the area of the greatest radionuclide concentration on the scan. In patients with aplastic anemia, there was good correlation between the plasma iron clearance t1/2 and the scan grade. Less agreement was found in the comparison between the Fe-59 sacral and organ counts and the red-cell iron utilization. In patients with myelofibrosis, there was poor correlation between the surface counts over the sacrum and the red-cell iron utilization. Plasma iron clearances were abnormally short and were unrelated to the transferrin saturation levels. Eighteen patients were studied several times to evaluate their responses to steroid therapy. In all, there was good correlation between the bone-marrow imaging, the erythrocyte cellularity, ferrokinetics, and the patient's response to therapy. Indium-111 bone-marrow imaging is useful both in evaluating marrow erythroid activity and in following the response to therapy in patients with these diseases.

Sayle, B.A.; Helmer, R.E.; Birdsong, B.A.; Balachandran, S.; Gardner, F.H.

1982-02-01

38

Red Sea  

Atmospheric Science Data Center

article title:  The Red Sea     View Larger Image ... Multi-angle Imaging SpectroRadiometer (MISR) image of the Red Sea was acquired on August 13, 2000. Located between the East African coast and the Saudi Arabian peninsula, the Red Sea got its name because the blooms of a type of algae,  Trichodesmium ...

2013-04-16

39

Inherited Bone Marrow Failure Syndromes  

MedlinePLUS

What are the IBMFS disorders? Amegakaryocytic Thrombocytopenia Diamond-Blackfan Anemia Dyskeratosis Congenita Fanconi Anemia Pearson Syndrome Severe Congenital Neutropenia Shwachman-Diamond Syndrome Thrombocytopenia Absent Radii Other Bone Marrow Failure Syndromes Amegakaryocytic ...

40

Aspiration and Biopsy: Bone Marrow  

MedlinePLUS

... aspiration and biopsy, you can speak with your nurse or doctor before the procedure. Reviewed by: Yamini Durani, MD Date reviewed: April ... Cell Transplants Anemia Biopsy Leukemia Word! Bone Marrow About ...

41

Red blood cell decreases of microgravity  

NASA Technical Reports Server (NTRS)

Postflight decreases in red blood cell mass (RBCM) have regularly been recorded after exposure to microgravity. These 5-25 percent decreases do not relate to the mission duration, workload, caloric intake or to the type of spacecraft used. The decrease is accompanied by normal red cell survivals, increased ferritin levels, normal radioactive iron studies, and increases in mean red blood cell volume. Comparable decreases in red blood cell mass are not found after bed rest, a commonly used simulation of the microgravity state. Inhibited bone marrow erythropoiesis has not been proven to date, although reticulocyte numbers in the peripheral circulation are decreased about 50 percent. To date, the cause of the microgravity induced decreases in RBCM is unknown. Increased splenic trapping of circulating red blood cells seem the most logical way to explain the results obtained.

Johnson, P. C.

1985-01-01

42

Red Tides  

NSDL National Science Digital Library

This comprehensive website answers questions such as: what is red tide, where are they found, why do they occur, how do they affect marine organisms, how do they affect humans, how are shellfish tested for the toxin, and what is being done to remediate the red tide problem. The site features color pictures and black and white maps.

Communications Directorate, Department O.

43

Red Sky with Red Mesa  

SciTech Connect

The Red Sky/Red Mesa supercomputing platform dramatically reduces the time required to simulate complex fuel models, from 4-6 months to just 4 weeks, allowing researchers to accelerate the pace at which they can address these complex problems. Its speed also reduces the need for laboratory and field testing, allowing for energy reduction far beyond data center walls.

None

2011-04-14

44

Red Sky with Red Mesa  

ScienceCinema

The Red Sky/Red Mesa supercomputing platform dramatically reduces the time required to simulate complex fuel models, from 4-6 months to just 4 weeks, allowing researchers to accelerate the pace at which they can address these complex problems. Its speed also reduces the need for laboratory and field testing, allowing for energy reduction far beyond data center walls.

None

2014-06-23

45

Olsalazine and 6-mercaptopurine-related bone marrow suppression: A possible drug-drug interaction  

Microsoft Academic Search

A patient with refractory Crohn's disease had two separate episodes of bone marrow suppression while receiving 50 to 75 mg 6-mercaptopurine a day and 1000 to 1750 mg olsalazine a day. This adverse reaction necessitated dose reduction of 6-mercaptopurine on the first occasion and withdrawal of 6-mercaptopurine and olsalazine on the second occasion. The patient's red blood cell thiopurine methyltransferase

Lionel D. Lewis; Carol L. Szumlanski; Diane M. Otterness; Lynne Lennard; Richard M. Weinshilboum; David W. Nierenberg

1997-01-01

46

Blood volume and red cell life span (M113), part C  

NASA Technical Reports Server (NTRS)

Prechamber, in-chamber, and postchamber blood samples taken from Skylab simulation crewmembers did not indicate significant shortening of the red cell life span during the mission. This does not suggest that the space simulation environment could not be associated with red cell enzyme changes. It does show that any changes in enzymes were not sufficiently great to significantly shorten red cell survival. There was no evidence of bone marrow erythropoetic suppression nor was there any evidence of increased red cell destruction.

Johnson, P. C., Jr.

1973-01-01

47

Red Clover  

MedlinePLUS

... et al. Phytoestrogens for vasomotor menopausal symptoms. Cochrane Database of Systematic Reviews . 2007;(4):CD001395. Nelson HD, ... 17):2057–2071. Red clover. Natural Medicines Comprehensive Database Web site. Accessed at www.naturaldatabase.com on ...

48

Planning for a Bone Marrow Transplant (BMT)  

MedlinePLUS

... Facebook Google Bookmarks Planning for a Bone Marrow Transplant (BMT) When a bone marrow transplant (also called ... Teenager Through His or Her Transplant Consider the Transplant Center You may want to have a discussion ...

49

Blood and Marrow Stem Cell Transplant  

MedlinePLUS

... What Is a Blood and Marrow Stem Cell Transplant? A blood and marrow stem cell transplant replaces ... replace the missing white blood cells. Types of Transplants The two main types of stem cell transplants ...

50

Marrow Fat and Bone—New Perspectives  

PubMed Central

Context: There is growing interest in the relationship between bone mineral density, bone strength, and fat depots. Marrow adipose tissue, a well-established component of the marrow environment, is metabolically distinct from peripheral fat depots, but its functional significance is unknown. Objective: In this review, we discuss animal and human data linking the marrow adipose tissue depot to parameters of bone density and integrity as well as the potential significance of marrow adipose tissue in metabolic diseases associated with bone loss, including type 1 diabetes mellitus and anorexia nervosa. Potential hormonal determinants of marrow adipose tissue are also discussed. Conclusions: We conclude that whereas most animal and human data demonstrate an inverse association between marrow adipose tissue and measures of bone density and strength, understanding the functional significance of marrow adipose tissue and its hormonal determinants will be critical to better understanding its role in skeletal integrity and the role of marrow adipose tissue in the pathophysiology of bone loss. PMID:23393168

Fazeli, Pouneh K.; Horowitz, Mark C.; MacDougald, Ormond A.; Scheller, Erica L.; Rodeheffer, Matthew S.; Rosen, Clifford J.

2013-01-01

51

A bone marrow toxicity model for 223Ra alpha-emitter radiopharmaceutical therapy  

PubMed Central

Purpose Ra-223, an ?-particle emitting bone-seeking radionuclide, has recently been used in clinical trials for osseous metasteses of prostate cancer. We investigated the relationship between absorbed fraction-based red marrow dosimetry and cell level-dosimetry using a model that accounts for the expected localization of this agent relative to marrow cavity architecture. We show that cell level-based dosimetry is essential to understanding potential marrow toxicity. Methods The GEANT4 software package was used to create simple spheres representing marrow cavities. Ra-223 was positioned on the trabecular bone surface or in the endosteal layer and simulated for decay, along with the descendants. The interior of the sphere was divided into cell-size voxels and the energy was collected in each voxel and interpreted as dose cell histograms. The average absorbed dose values and absorbed fractions were also calculated in order to compare those results with previously published values. Results The absorbed dose was predominantly deposited near the trabecular surface. The dose cell histograms results were used to plot the percentage of cells that received a potentially toxic absorbed dose (2 or 4 Gy) as a function of the average absorbed dose over the marrow cavity. The results show (1) a heterogeneous distribution of cellular absorbed dose, strongly dependent on the position of the cell within the marrow cavity; and (2) that increasing the average marrow cavity absorbed dose, or equivalently, increasing the administered activity resulted in only a small increase in potential marrow toxicity (i.e., the number of cells receiving more than 4 or 2 Gy), for a range of average marrow cavity absorbed doses from 1 Gy to 20 Gy. Conclusion The results from the trabecular model differ markedly from a standard absorbed fraction method while presenting comparable average dose values. These suggest that increasing the amount of radioactivity may not substantially increase the risk of toxicity, a result unavailable to the absorbed fraction method of dose calculation. PMID:22546715

Hobbs, Robert F; Song, Hong; Watchman, Christopher J; Bolch, Wesley E; Aksnes, Anne-Kirsti; Ramdahl, Thomas; Flux, Glenn D; Sgouros, George

2012-01-01

52

Fatty marrow conversion of the proximal femoral metaphysis in transient bone marrow edema syndrome  

Microsoft Academic Search

Introduction In the proximal femoral meta- physis, hematopoietic marrow is predominant during the adult stage of life. The conversion of hematopoietic marrow to fatty marrow in the proximal femoral meta- physis has been suggested as an etiologic factor of ischemia in the pathogenesis of femoral head osteone- crosis. To determine whether the chronology of fatty marrow conversion of the proximal

Shin-Yoon Kim; Kyung-Hoi Koo; Kuen-Tak Suh; Yong-Sik Kim; Yoon-Je Cho; Byung-Woo Min; Yong-Chan Ha; Jung-Ah Choi

2005-01-01

53

Neocytolysis: physiological down-regulator of red-cell mass  

NASA Technical Reports Server (NTRS)

It is usually considered that red-cell mass is controlled by erythropoietin-driven bone marrow red-cell production, and no physiological mechanisms can shorten survival of circulating red cells. In adapting to acute plethora in microgravity, astronauts' red-cell mass falls too rapidly to be explained by diminished red-cell production. Ferrokinetics show no early decline in erythropolesis, but red cells radiolabelled 12 days before launch survive normally. Selective destruction of the youngest circulating red cells-a process we call neocytolysis-is the only plausible explanation. A fall in erythropoietin below a threshold is likely to initiate neocytolysis, probably by influencing surface-adhesion molecules. Recognition of neocytolysis will require re-examination of the pathophysiology and treatment of several blood disorders, including the anaemia of renal disease.

Alfrey, C. P.; Rice, L.; Udden, M. M.; Driscoll, T. B.

1997-01-01

54

Blood and Marrow Transplant (BMT) Program  

E-print Network

Blood and Marrow Transplant (BMT) Program Norris Cotton Cancer Center 000020200201111112-24747474 #12;Welcome On behalf of the members of the Blood and Marrow Transplant Team, we offer you a warm. - Kenneth Meehan, MD Director of the Blood & Marrow Transplantation Why this booklet is for you This booklet

Myers, Lawrence C.

55

Bone marrow and the control of immunity  

Microsoft Academic Search

Bone marrow is thought to be a primary hematopoietic organ. However, accumulated evidences demonstrate that active function and trafficking of immune cells, including regulatory T cells, conventional T cells, B cells, dendritic cells, natural killer T (NKT) cells, neutrophils, myeloid-derived suppressor cells and mesenchymal stem cells, are observed in the bone marrow. Furthermore, bone marrow is a predetermined metastatic location

Ende Zhao; Huanbin Xu; Lin Wang; Ilona Kryczek; Ke Wu; Yu Hu; Guobin Wang; Weiping Zou

2012-01-01

56

Cell Stem Cell Endogenous Bone Marrow MSCs  

E-print Network

). The existence of multipotent bone marrow stromal cells (BMSCs), or skeletal/mesenchymal stem cells (SSCs of cultured cells (Sacchetti et al., 2007). Similar multipotent MSCs can be isolated from mouse bone marrowCell Stem Cell Article Endogenous Bone Marrow MSCs Are Dynamic, Fate-Restricted Participants

Mootha, Vamsi K.

57

Bone Marrow Aspiration and Biopsy  

MedlinePLUS

... Deficiency , Hemoglobin Abnormalities , Bleeding Disorders Elsewhere On The Web College of American Pathologists: MyBiopsy.org American Society of Hematology: Blood Basics National Heart, Lung and Blood Institute: What are bone marrow tests? National Heart, Lung and Blood Institute: Blood Diseases ...

58

Combined Simulation and Experimental Study of Large Deformation of Red Blood Cells in Microfluidic Systems  

E-print Network

Combined Simulation and Experimental Study of Large Deformation of Red Blood Cells in Microfluidic blood cells (RBCs) traversing microfluidic channels with cross-sectional areas as small as 2.7 9 3 lm During its typical life span of 120 days upon egress from the bone marrow, the human red blood cell (RBC

Dao, Ming

59

Management of thrombocytopenia in bone marrow failure: a review.  

PubMed

The clinical course of many neoplastic and primary bone marrow diseases will result in cytopenias secondary to bone marrow failure or infiltration. Acute and chronic leukemias, the myelodysplastic syndromes (MDS), aplastic anemia, breast and prostate cancer, as well as other hematologic and solid tumors, all may lead to chronic, severe cytopenias. Management of anemia and neutropenia are well described in the medical literature. Less well detailed are management approaches for patients with chronic thrombocytopenia, with or without active bleeding. Severe thrombocytopenia presents many difficult management choices for caregivers, patients and their families, especially near the end of life. The use of platelet transfusions in this patient population presents complex issues; platelets are logistically more difficult to transfuse than red cells and carry risks including acute febrile episodes, alloimmunization, and infection. In this review, we discuss the association of chronic thrombocytopenia to serious bleeding and the role of various prophylactic and therapeutic interventions available to palliative care and hospice providers. Specifically, this review examines the following issues: What is the morbidity and mortality from chronic thrombocytopenia in the setting of cancer or other bone marrow failure states? Is there a role for prophylactic platelet transfusions in the palliative care setting, and if so, with what frequency of monitoring, and at what transfusion threshold? What is the impact of alloimmunization and how can it be minimized? What treatments are available besides, or in addition to, platelet transfusions for acute bleeding episodes? PMID:17298272

Salacz, Michael E; Lankiewicz, Michael W; Weissman, David E

2007-02-01

60

Bone Marrow Microenvironment and Tumor Progression  

PubMed Central

The bone marrow constitutes an unique microenvironment for cancer cells in three specific aspects. First, the bone marrow actively recruits circulating tumor cells where they find a sanctuary rich in growth factors and cytokines that promote their proliferation and survival. When in the bone marrow, tumor cells profoundly affect the homeostasis of the bone and the balance between osteogenesis and osteolysis. As a consequence, growth and survival factors normally sequestered into the bone matrix are released, further fueling cancer progression. Second, tumor cells actively recruit bone marrow-derived precursor cells into their own microenvironment. When in the tumors, these bone marrow-derived cells contribute to an inflammatory reaction and to the formation of the tumor vasculature. Third, bone marrow-derived cells can home in distant organs, where they form niches that attract circulating tumor cells. Our understanding of the contribution of the bone marrow microenvironment to cancer progression has therefore dramatically improved over the last few years. The importance of this new knowledge cannot be underestimated considering that the vast majority of cancer treatments such as cytotoxic and myeloablative chemotherapy, bone marrow transplantation and radiation therapy inflict a trauma to the bone marrow microenvironment. How such trauma affects the influence that the bone marrow microenvironment exerts on cancer is still poorly understood. In this article, the reciprocal relationship between the bone marrow microenvironment and tumor cells is reviewed, and its potential impact on cancer therapy is discussed. PMID:19308682

Chantrain, Christophe F.; Feron, Olivier; Marbaix, Etienne

2008-01-01

61

Immune transfer studies in canine allogeneic marrow graft donor-recipient pairs  

SciTech Connect

Transfer of immunity occurring with bone marrow grafting was studied using the dog as a preclinical model. Allogeneic bone marrow transplantation (BMT) was performed between DLA-identical beagle litter-mates. The donors were immunized with tetanus toxoid (TT) or sheep red blood cells (SRBC), and their humoral response was monitored by hemagglutination. The recipients of bone marrow from TT-immunized donors showed a marked increase of antibody titer one week posttransplantation, while in the recipients of marrow from SRBC immunized donors the antibody titers were considerably lower. Within the following 60 days the antibody titers in both groups diminished gradually to pregrafting levels. Control experiments in which cell-free plasma from donors immunized with TT and SRBC respectively was transfused indicated that the initial rise of specific antibody titers after marrow grafting is likely to be due to a passive transfer of humoral immunity. A single challenge of these marrow graft recipients with the respective antigen 15-18 weeks posttransplantation led to a secondary type of humoral immune response. It could be demonstrated that transfer of memory against TT or SRBC was independent from the actual antibody titer and the time of vaccination of the donor. One dog was immunized with TT after serving as marrow donor. When the donor had shown an antibody response, a peripheral blood leukocytes (PBL) transfusion was given to his chimera. Subsequent challenge of the latter resulted in a secondary type of specific antibody response. This indicates that specific cellular-bound immunological memory can be transferred after BMT from the donor to his allogeneic bone marrow chimera by transfusion of peripheral blood leukocytes. The data may be of importance in clinical BMT to protect patients during the phase of reduced immune reactivity by transfer of memory cells.

Grosse-Wilde, H.; Krumbacher, K.; Schuening, F.D.; Doxiadis, I.; Mahmoud, H.K.; Emde, C.; Schmidt-Weinmar, A.; Schaefer, U.W.

1986-07-01

62

Bone marrow: the workhorse organ.  

PubMed

Bone marrow accounts for 3% to 6% of body weight and is dispersed throughout bone. In a healthy adult, bone marrow produces approximately 500 billion new blood cells daily to maintain steady-state levels in the peripheral circulation. Its output is measured using the complete blood count (CBC), which is arguably the most frequently ordered laboratory test in the clinical milieu. Hematopoiesis starts with hematopoietic stem cells that differentiate and mature into any of the three different types of mature blood cells. A simple CBC can rule out, confirm, or direct attention to anemia, cancer, infection, acute hemorrhage states, toxin exposure, allergies, immunodeficiencies, or adverse drug reactions. Interpreting CBCs depends on reference values from the laboratory that processes the blood sample. Since reference intervals are formulated based on a younger, much healthier population, data specific to elderly people are limited. PMID:23315278

Wick, Jeannette Y

2013-01-01

63

Bone marrow transplantation from a cadaveric donor.  

PubMed

A 2.5-year-old girl with neurogenic Gaucher's disease was transplanted with donor bone marrow from her HLA-compatible 12-year-old brother whose marrow was harvested 30 min post-mortem, after he suffered a severe head and neck injury. The marrow was stored in liquid nitrogen for 30 days prior to infusion. The post-transplantation period was uneventful with good engraftment and no signs of graft-versus-host disease. Currently, 6 months post-allogeneic bone marrow transplantation (alloBMT), analysis of both bone marrow and blood samples by PCR documented only cells of donor origin. This case demonstrates the feasibility of cadaveric marrow as a source of donor cells. To our knowledge, this patient is the only survivor of alloBMT from a cadaveric donor. PMID:9603417

Kapelushnik, J; Aker, M; Pugatsch, T; Samuel, S; Slavin, S

1998-04-01

64

Bone Marrow Microenvironment and Tumor Progression  

Microsoft Academic Search

The bone marrow constitutes an unique microenvironment for cancer cells in three specific aspects. First, the bone marrow\\u000a actively recruits circulating tumor cells where they find a sanctuary rich in growth factors and cytokines that promote their\\u000a proliferation and survival. When in the bone marrow, tumor cells profoundly affect the homeostasis of the bone and the balance\\u000a between osteogenesis and

Christophe F. Chantrain; Olivier Feron; Etienne Marbaix; Yves A. DeClerck

2008-01-01

65

Effects of glucan on bone marrow  

PubMed Central

Bone marrow damage represents a significant problem in cancer treatment. Therefore, it is clear that the pharmacologic protection against bone marrow damage is of considerable interest, since the development of novel and effective medical approaches to combat radiation or cytotoxic damage are of major importance not only to the medical field but also to several industries and the military. This review represents a summary of our knowledge of the effects of various glucans on bone marrow protection. PMID:25332994

Vannucci, Luca; Vetvicka, Vaclav

2014-01-01

66

Bone marrow iron in nutritional anaemias.  

PubMed

Bone marrow smears of 168 patients with nutritional anaemias attending the Dr. J.C. Patel, Department of Hematology, K.E.M. Hospital were stained by Prussian blue method for iron (haemosiderin). Iron in the bone marrow was classified as absent, decreased, normal or increased. Amongst 93 cases with transferrin saturation (TS) of less than 16% and normoblastic erythropoiesis, bone marrow iron was absent in 48 (51.6%) and decreased in 45 (48.4%). In 50 cases with TS of less than 16% and marrow showing megaloblasts and/or giant myelocytes and metamyelocytes, bone marrow iron was absent in 15 (30%), decreased in 22 (44%), normal in 7 (14%) and increased in 6 (12%). In 25 cases with TS over 16% and megaloblastic erythropoiesis, bone marrow iron was absent in 4 (16%), decreased in 1 (4%), normal in 7 (28%) and increased in 13 (52%). In 150 (89.3%) patients out of 168, bone marrow iron and TS gave concordant results whereas in 18 (10.7%), the results were discordant; former was encountered in cases of uncomplicated iron deficiency while latter was found with megaloblastic morphology of the marrow. It is concluded that there is a good correlation between TS and bone marrow iron and hence, either of the criteria can be used for the diagnosis of iron deficiency especially when it is not complicated by megaloblastosis. PMID:2632529

Mehta, B C; Pandya, B G

1989-11-01

67

Red Files  

NSDL National Science Digital Library

Red Files, a four-part documentary series from PBS, utilizes previously unavailable archival sources and interviews to present a fascinating look at the Soviet Union and its Cold War rivalry with the US. This attractive companion site offers a number of resources related to each of the four episodes: Secret Victories of the KGB, Soviet Sports Wars, Secret Soviet Moon Mission, and Soviet Propaganda. For each installment, users will find a story synopsis, the Producer's script, theme music, updates on related events, human interest stories, complete interview transcripts, video clips, a reference section, access to related sections of Russian Archives Online, maps, a timeline, lesson plans, and more. Additional offerings include a collection of links mentioned in the series and an internal search engine. This site joins an already strong tradition at PBS of creating sites that are actual companions to the program, offering new and expanded content for interested users.

68

Finding Red  

NSDL National Science Digital Library

In this chemistry challenge, learners systematically investigate which combination of four solutions produces a deep red color. The four solutions are iron(III) chloride, ammonium thiocyanate, tannic acid, and oxalic acid. Background information explains that it is the iron ions in solution combining with ions from the other solutions to create the different colors. After learners discover the different colors, they are encouraged to add a third solution to see if the color can be changed, an example of how chemical equilibrium can be shifted. This activity may take a bit more time with younger learners. For safety reasons, adult supervision is recommended and can be conducted as a demonstration for younger audiences.

Sciencenter

2014-08-27

69

Legal issues in bone marrow transplantation.  

PubMed Central

The article discusses some of the more common legal issues involved in bone marrow transplantation. These include malpractice claims, testing prospective donors for AIDS, sale of bone marrow, informed consent for both donor and recipient, and questions that arise when the donor is a child. PMID:2293512

Holder, A. R.

1990-01-01

70

Liver from Bone Marrow in Humans  

Microsoft Academic Search

It has been shown in animal models that hepatocytes and cholangiocytes can derive from bone marrow cells. We have investigated whether such a process occurs in humans. Archival autopsy and biopsy liver specimens were obtained from 2 female recipients of therapeutic bone marrow transplantations with male donors and from 4 male recipients of orthotopic liver transplantations from female donors. Immunohistochemical

Neil D. Theise; Manjunath Nimmakayalu; Rebekah Gardner; Peter B. Illei; Glyn Morgan; Lewis Teperman; Octavian Henegariu; Diane S. Krause

2000-01-01

71

[Disseminated bone marrow metastases from gastric cancer: detection and monitoring the effectiveness of chemotherapy by bone marrow scintigraphy].  

PubMed

Disseminated bone marrow metastasis of cancer is a critical condition, frequently complicated by disseminated intravascular coagulation (DIC). A 32-year-old man with gastric cancer was diagnosed as having disseminated bone marrow metastases. Bone scintigraphy demonstrated many abnormal radionuclide accumulations in the whole body. Bone marrow aspiration revealed cancer cells. Bone marrow scintigraphy with 111In-Cl3 demonstrated central marrow failure and peripheral expansion. The remission of DIC was observed after sequential methotrexate and 5-FU therapy, then uptake of radionuclide in the central bone marrow was remarkably improved by bone marrow scan. After thirteen anti-cancer chemotherapies, recurrence of DIC was suspected because of the reduction of blood platelet count. Nevertheless, repeated bone marrow scan still demonstrated the central bone marrow clearly. The patient discharged from our hospital without the recurrence of DIC. We considered bone marrow scintigraphy is useful in the detection of disseminated bone marrow metastases of cancer and monitoring the effectiveness of chemotherapy. PMID:11452490

Imai, Y; Asakura, Y; Kinoshita, M; Sueyoshi, T; Eguchi, Y; Oota, S; Fujiwara, K; Suzuki, K; Miyamae, T

2001-05-01

72

Inherited Bone Marrow Failure Syndromes (IBMFS)  

Cancer.gov

A study of inherited bone marrow failure syndromes (IBMFS), a group of rare genetic blood disorders that include Fanconi Anemia, Dyskeratosis Congenita, Diamond-Blackfan Anemia, Shwachman-Diamond Syndrome, Severe Congenital Neutropenia, Amegakaryocytic Thrombocytopenia, and Thrombocytopenia Absent Radii.

73

Transplant Outcomes (Bone Marrow and Cord Blood)  

MedlinePLUS

... survival and transplant data of bone marrow and umbilical cord blood transplants in the transplant outcomes registry of the C.W. Bill Young Cell Transplantation Program (Program), also called the Stem Cell Therapeutic ...

74

Bone marrow lesions: A systematic diagnostic approach  

PubMed Central

Bone marrow lesions on magnetic resonance (MR) imaging are common and may be seen with various pathologies. The authors outline a systematic diagnostic approach with proposed categorization of various etiologies of bone marrow lesions. Utilization of typical imaging features on conventional MR imaging techniques and other problem-solving techniques, such as chemical shift imaging and diffusion-weighted imaging (DWI), to achieve accurate final diagnosis has been highlighted. PMID:25114392

Grande, Filippo Del; Farahani, Sahar J; Carrino, John A; Chhabra, Avneesh

2014-01-01

75

Allogeneic Bone Marrow Transplantation in Multiple Myeloma  

Microsoft Academic Search

A 28-year-old woman with Bence-Jones multiple myeloma (MM) presented with several osteolytic lesions and a massive bone marrow infiltration with mature plasmocytes. After 6 cycles of chemotherapy with melphalan and prednisone, the patient, in apparent clinical remission, underwent allogeneic bone marrow transplantation (BMT) from her brother. The patient has been followed up for 23 months, showing complete remission by clinical

A. Gallamini; F. Buffa; A. Bacigalupo; M. T. Van Lint; J. Peralvo; P. A. Pittaluga; D. Occhini; A. M. Marmont

1987-01-01

76

Repair of orbital bone defects in canines using grafts of enriched autologous bone marrow stromal cells  

PubMed Central

Backgroud Bone tissue engineering is a new approach for the repair of orbital defects. The aim of the present study was to explore the feasibility of tissue-engineered bone constructed using bone marrow stromal cells (BMSCs) that were rapidly isolated and concentrated from bone marrow (BM) by the red cell lysis method, then combined with ?-tricalcium phosphate (?-TCP) to create grafts used to restore orbital bone defects in canines. Methods In the experimental group, grafts were constructed using BMSCs obtained by red cell lysis from 20 ml bone marrow, combined with ?-TCP and BM via the custom-made stem cell-scaffold device, then used to repair 10 mm diameter medial orbital wall bony defects in canines. Results were compared with those in groups grafted with BM/?-TCP or ?-TCP alone, or with defects left untreated as controls. The enrichment of BMSCs and nucleated cells (NCs) in the graft was calculated from the number in untreated bone marrow and in suspensions after red cell lysis. Spiral computed tomography (CT) scans were performed 1, 4, 12 and 24 weeks after implantation in all groups. Gross examination, micro-CT and histological measurements were performed 24 weeks after surgery. The results were analyzed to evaluate the efficacy of bone repair. Results The number of NCs and of colony-forming units within the scaffolds were increased 54.8 times and 53.4 times, respectively, compared with untreated bone marrow. In the BMSC-BM/?-TCP group, CT examination revealed that the scaffolds were gradually absorbed and the bony defects were restored. Micro-CT and histological examination confirmed that the implantations led to good repair of the defects, with 6 out 8 orbital defects completely restored in the experimental group, while by contrast, the grafts in the control groups did not fully repair the bony defects, a difference which was statistically significant (p?red cell lysis of BM, can restore critical-sized orbital wall defects in canines. PMID:24886296

2014-01-01

77

Primary splenic diffuse large B-cell lymphoma manifesting in red pulp  

Microsoft Academic Search

We evaluated six cases of diffuse large B-cell lymphoma (DLBCL) involving the red pulp of the spleen. All had B symptoms and\\u000a an aggressive clinical course. The lymphoma cells proliferated diffusely and non-cohesively in the cords of the red pulp.\\u000a The lymphoma involved the bone marrow in four of the five patients and the liver in all four of the

Makoto Kashimura; Masahiro Noro; Bunshiro Akikusa; Atsushi Okuhara; Shuji Momose; Ikuo Miura; Masaru Kojima; Jun-ichi Tamaru

2008-01-01

78

Fabrication of biofunctionalized, cell-laden macroporous 3D PEG hydrogels as bone marrow analogs for the cultivation of human hematopoietic stem and progenitor cells.  

PubMed

In vitro proliferation of hematopoietic stem cells (HSCs) is yet an unresolved challenge. Found in the bone marrow, HSCs can undergo self-renewing cell division and thereby multiply. Recapitulation of the bone marrow environment in order to provide the required signals for their expansion is a promising approach.Here, we describe a technique to produce biofunctionalized, macroporous poly(ethylene glycol) diacrylate (PEGDA) hydrogels that mimic the spongy 3D architecture of trabecular bones, which host the red, blood-forming bone marrow. After seeding these scaffolds with cells, they can be used as simplified bone marrow analogs for the cultivation of HSCs. This method can easily be conducted with standard laboratory chemicals and equipment. The 3D hydrogels are produced via salt leaching and biofunctionalization of the material is achieved by co-polymerizing the PEGDA with an RGD peptide. Finally, cell seeding and retrieval are described. PMID:24875249

Rödling, Lisa; Raic, Annamarija; Lee-Thedieck, Cornelia

2014-01-01

79

Bone marrow blood vessel ossification and "microvascular dead space" in rat and human long bone.  

PubMed

Severe calcification of the bone microvascular network was observed in rats, whereby the bone marrow blood vessels appeared ossified. This study sought to characterize the magnitude of ossification in relation to patent blood vessels and adipocyte content in femoral diaphyses. Additionally, this study confirmed the presence of ossified vessels in patients with arteriosclerotic vascular disease and peripheral vascular disease and cellulitis. Young (4-6 month; n=8) and old (22-24 month; n=8) male Fischer-344 rats were perfused with barium sulfate to visualize patent bone marrow blood vessels. Femoral shafts were processed for bone histomorphometry to quantify ossified (Goldner's Trichrome) and calcified (Alizarin Red) vessels. Adipocyte content was also determined. Additional femora (n=5/age group) were scanned via ?CT to quantify microvascular ossification. Bone marrow blood vessels from the rats and the human patients were also isolated and examined via microscopy. Ossified vessels (rats and humans) had osteocyte lacunae on the vessel surfaces and "normal" vessels were transitioning into bone. The volume of ossified vessels was 4800% higher (p<0.05) in the old vs. young rats. Calcified and ossified vessel volumes per tissue volume and calcified vessel volume per patent vessel volume were augmented (p<0.05) 262%, 375% and 263%, respectively, in the old vs. young rats. Ossified and patent vessel number was higher (171%) and lower (40%), respectively, in the old vs. young rats. Finally, adipocyte volume per patent vessel volume was higher (86%) with age. This study is the first to report ossification of bone marrow blood vessels in rats and humans. Ossification presumably results in "microvascular dead space" in regard to loss of patency and vasomotor function as opposed to necrosis. Progression of bone microvascular ossification may provide the common link associated with age-related changes in bone and bone marrow. The clinical implications may be evident in the difficulties treating bone disease in the elderly. PMID:24680721

Prisby, Rhonda D

2014-07-01

80

Bone Marrow Injury Induced via Oxidative Stress in Mice by Inhalation Exposure to Formaldehyde  

PubMed Central

Objective Formaldehyde, a ubiquitous environmental pollutant has been classified as a human leukemogen. However, toxicity of formaldehyde in bone marrow, the target site of leukemia induction, is still poorly understood. Methodology/Principal Findings To investigate bone marrow toxicity (bone marrow pathology, hematotoxicity) and underlying mechanisms (oxidative stress, inflammation, apoptosis) in formaldehyde-exposed mice. Male Balb/c mice were exposed to formaldehyde (0, 0.5, and 3.0 mg/m3) by nose-only inhalation for 8 hours/day, over a two week period designed to simulate a factory work schedule, with an exposure-free “weekend” on days 6 and 7, and were sacrificed on the morning of day 13. Counts of white blood cells, red blood cells and lymphocytes were significantly (p<0.05) decreased at 0.5 mg/m3 (43%, 7%, and 39%, respectively) and 3.0 mg/m3 (52%, 27%, and 43%, respectively) formaldehyde exposure, while platelet counts were significantly increased by 109% (0.5 mg/m3) and 67% (3.0 mg/m3). Biomarkers of oxidative stress (reactive oxygen species, glutathione depletion, cytochrome P450 1A1 and glutathione s-transferase theta 1 expression), inflammation (nuclear factor kappa-B, tomour necrosis factor alpha, interleukin-1 beta), and apoptosis (activity of cysteine-aspartic acid protease 3) in bone marrow tissues were induced at one or both formaldehyde doses mentioned above. Conclusions/Significance Exposure of mice to formaldehyde by inhalation induced bone marrow toxicity, and that oxidative stress, inflammation and the consequential apoptosis jointly constitute potential mechanisms of such induced toxicity. PMID:24040369

McHale, Cliona; Li, Rui; Zhang, Luoping; Wu, Yang; Ye, Xin; Yang, Xu; Ding, Shumao

2013-01-01

81

Specific marrow ablation before marrow transplantation using an aminophosphonic acid conjugate 166Ho-EDTMP.  

PubMed

166Holmium ethylenediaminetetramethylene phosphonic acid (166Ho-EDTMP) is a short-lived beta-emitting radionuclide complexed to an aminophosphonate ligand that we have investigated in a canine model as a potential agent for specific marrow ablation before marrow transplantation. After intravenous injections, 166Ho-EDTMP distributed principally to bone and after 24 hours the concentrations of 166Ho-EDTMP in bone were more than 200-fold higher than in any other organ. Increasing dosages of 166Ho-EDTMP led to increasingly prolonged and severe myelosuppression, but myeloablation was not achieved. Histologic examination of recovering animals suggested that the spleen may have acted as a reservoir for circulatory hematopoietic precursors. Four splenectomized animals administered 20 to 30 mCi/kg 166Ho-EDTMP without marrow transplantation died with marrow aplasia, while four splenectomized animals administered similar dosages of 166Ho-EDTMP followed by autologous transplantation recovered. The dose-limiting toxicity of 166Ho-EDTMP appeared to be marrow stromal damage resulting in myelofibrosis, which was reversible. These results suggest that 166Ho-EDTMP can be used to specifically ablate marrow function before marrow transplantation. PMID:1520886

Appelbaum, F R; Brown, P A; Sandmaier, B M; Storb, R; Fisher, D R; Shulman, H M; Graham, T C; Schuening, F G; Deeg, H J; Bianco, J A

1992-09-15

82

To Evaluate the Role of Bone Marrow Aspiration and Bone Marrow Biopsy in Pancytopenia  

PubMed Central

Background: Pancytopenia is not a disease entity but a triad of findings that may result from various disease processes, primarily or secondarily involving the bone marrow. Bone marrow aspiration and biopsy evaluation along with good clinical correlation is of utmost importance to evaluate the causes of pancytopenia and planning further investigations. Aims: The present study was a prospective clinicohaematological study undertaken to analyse the various causes of pancytopenia by evaluating bone marrow aspiration and biopsy and correlating with clinical findings, complete blood counts and peripheral blood picture. Materials and Methods: Fifty patients of pancytopenia were included in the study in which relevant history and physical examination findings were recorded. Bone marrow aspiration and biopsy were performed simultaneously in all cases. Perl’s stain was done in all cases and special stains like MPO, PAS and reticulin were also done wherever necessary. Results and Conclusion: The maximum cases of pancytopenia were in the age group of 10 to 30 y with male preponderance. Aplastic anaemia was found to be the most common aetiology of pancytopenia followed by normoblastic erythroid hyperplasia, megaloblastic anaemia, acute leukemias, myelofibrosis, lymphoid neoplasia and iron deficiency anaemia. It was concluded from the study that although the advantages of bone marrow aspiration and biopsy differ, both are complimentary to each other and should be performed simultaneously for a complete bone marrow work up and evaluation. It is only through the correlation of clinical, hematological and bone marrow examination findings that proper evaluation and management of patients of pancytopenia can be made. PMID:25584228

Desalphine, Melina; Gupta, Parmod Kumar; Kataria, Amarjit Singh

2014-01-01

83

Lifeguarding American Red Cross  

E-print Network

Lifeguarding Manual #12;American Red Cross Lifeguarding Manual The following organizations provided review of the materials and/or support American Red Cross Lifeguarding: #12;This manual is part of the American Red Cross Lifeguarding program. By itself, it does not constitute complete and comprehensive

Carter, John

84

RED-LETTER DAYS  

EPA Science Inventory

The word "red-letter" is an adjective meaning "of special significance." It's origin is from the practice of marking Christian holy days in red letters on calendars. The "red-letter days" to which I refer occurred while I was a graduate student of ...

85

The effect of Arbas Cashmere goat bone marrow stromal cells on production of transgenic cloned embryos.  

PubMed

The aim of this study was to develop a method for the in vitro separation and culture of Arbas Cashmere goat bone marrow stromal cells (gBMSCs). Arbas Cashmere gBMSCs were isolated and cultured in vitro, and cell surface markers were identified immunohistochemically. The gBMSCs were differentiated into neurocytes and osteoblasts, and the expression of neuron-specific enolase and osteocalcin was identified by immunohistochemistry. The gBMSCs and goat fetal fibroblast cells (gFFCs) were compared for transient transfection efficiency and fluorescent colony-forming efficiency with Arbas Cashmere gFFCs as a control. pDsRed2-1 encodes DsRed2, a variant of the Discosoma sp. red fluorescent protein (DsRed). In addition, the coding sequence for DsRed2 contains a series of silent base-pair changes for higher expression in mammalian cells. Of the gBMSCs-pDsRed2-1, one fraction was tested for pluripotency, whereas the other fraction was manipulated using somatic cell nuclear transfer, and the in vitro growth status of transgenic embryos derived from gBMSCs-pDsRed2-1 and gFFCs-pDsRed2-1 was compared. The findings showed that gBMSCs were isolated and amplified to express CD29, CD44, and CD90 through adherent culture, with no marked signs of aging after multiple passages. Expression of neuron-specific enolase and osteocalcin by gBMSCs and gBMSCs-pDsRed2-1 was strongly induced by neuronal and osteogenic differentiation, whereas the integrated exogenous genes did not influence pluripotency (P > 0.05). The transient transfection efficiencies of gBMSCs and gFFCs after 48 hours were not significantly different; however, the fluorescent colony-forming efficiency of gBMSCs-pDsRed2-1 after G418 screening was approximately 13% higher than that of gFFCs-pDsRed2-1. The convergence and cleavage rates of cloned embryos derived from gBMSCs-pDsRed2-1 were higher than those derived from gFFCs-pDsRed2-1, whereas their eight-cell and blastocyst rates were similar. The red fluorescent protein expression levels were higher in transgenic embryos derived from gBMSCs-pDsRed2-1 compared with those derived from gFFCs-pDsRed2-1 (48.8% vs. 31.1%, respectively) (P < 0.01). Real-time quantitative Polymerase Chain Reaction analysis showed that DsRed2-1 messenger RNA expression of cloned embryos derived from gBMSCs was 2.24 greater than that of embryos derived from gFFCs-pDsRed2-1 (P < 0.01). Similarly, Western blot analysis showed that DsRed2 protein expression of cloned embryos derived from gBMSCs-pDsRed2-1 was 1.29 greater than that of embryos derived from gFFCs-pDsRed2-1 (P < 0.01). In this study, gBMSCs were also used for somatic cell nuclear transfer and shown to provide effective nuclear donor cells for breeding new genetically modified varieties of livestock. PMID:24679907

Ren, Yu; Wu, Haiqing; Wang, Hefei; Wang, Xiao; Liang, Hao; Liu, Dongjun

2014-06-01

86

The Red Kite  

NSDL National Science Digital Library

The Gigrin, a family-run sheep farm in Wales with a conservation mindset, offers this Web site devoted to the red kite, a small raptor that humans have attempted to protect longer than for any other bird species in the world. Readers can expect a solid introduction to red kite natural history, and an encouraging example of conservation by private landowners. The main Web page provides a general background information about the red kite, including a short audio clip of a red kite call. The following pages contain photos, detailed descriptions of nesting and feeding habits, and links to other organizations involved in protecting the red kite.

87

The Red Kite  

NSDL National Science Digital Library

The Gigrin, a family-run sheep farm in Wales with a conservation mindset, offers this Web site devoted to the red kite, a small raptor that humans have attempted to protect longer than for any other bird species in the world. Readers can expect a solid introduction to red kite natural history, and an encouraging example of conservation by private landowners. The main Web page provides a general background information about the red kite, including a short audio clip of a red kite call. The following pages contain photos, detailed descriptions of nesting and feeding habits, and links to other organizations involved in protecting the red kite.

2008-06-13

88

25 YEARS OF PROGRESS IN BLOOD & MARROW TRANSPLANTATION  

E-print Network

25 YEARS OF PROGRESS IN BLOOD & MARROW TRANSPLANTATION Stanford University Division of Blood & Marrow Transplantation and The Stanford Cancer Center invite you to Li Ka Shing Center for Learning in Blood & Marrow Transplantation, will offer attendees an historical overview of the tremendous advances

Bejerano, Gill

89

Pure red cell aplasia associated with systemic lupus erythematosus.  

PubMed

Pure red cell aplasia is a rare condition described in patients with autoimmune diseases such as systemic lupus erythematosus. Bone marrow examination of a 52-year-old female showed selective severe hypoplasia, scarce hematopoietic reserves, and no abnormality in other cell lineages, which are findings compatible with red cell aplasia. This condition has not responded to corticosteroids, cytotoxic drugs or intravenous immunoglobulin. After therapy with high doses of glucocorticoids, cyclophosphamide, and immunoglobulin failed, she was treated with human recombinant erythropoietin, monthly pulses of methylprednisolone, and cyclophosphamide, simultaneously. Data on treatment with erythropoietin for pure red cell aplasia associated with systemic lupus erythematosus is limited, but it appears to be reasonable to try in refractory cases. PMID:25326408

Koyama, R V; Silva, L F; Henriques, V B; Tran, C; Yoshikawa, G T

2014-01-01

90

Dynamic Visualization of Thrombopoiesis Within Bone Marrow  

Microsoft Academic Search

Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by

Tobias Junt; Harald Schulze; Zhao Chen; Steffen Massberg; Tobias Goerge; Andreas Krueger; Denisa D. Wagner; Thomas Graf; Joseph E. Italiano; Ramesh A. Shivdasani; Ulrich H. von Andrian

2007-01-01

91

Allogeneic and Autologous Bone-Marrow Transplantation  

PubMed Central

The author of this paper presents an overview of the current status of bone marrow transplantation, including indications, pre-transplant considerations, the transplant procedure, acute and delayed transplant-related problems, results currently attainable, and a short discussion of possible future developments. PMID:21253121

Deeg, H. Joachim

1988-01-01

92

[Transient bone marrow edema of the hip].  

PubMed

Transient bone marrow edema of the hip is characterized by moderate homogeneous low MR signal intensity with ill-defined margins that involves at least a portion of the femoral head. Spin echo T1-weighted images are helpful to exclude other underlying diseases (tumor, infection, necrosis from systemic origin…), for which marrow edema is secondary or no epiphyseal in location. High-resolution fat-suppressed T2-weighted or proton density images allow evaluation of the articular cartilage, subchondral bone and subchondal marrow: if the articular cartilage is abnormal, the lesion is irreversible (arthrosis or necrosis); if the subchondral bone is focally interrupted and/or if the femoral is no longer spherical, the lesion is irreversible (necrosis); if a focal linear fluid collection is present under the subchondral bone, the lesion is irreversible (necrosis). Finally, subchondral changes may provide useful prognostic information: the absence of any abnormality other than marrow edema typically indicates that complete resolution is likely; the presence of a focal T2-weighted hypointense lesion immediately next to the subchondral bone suggests an irreversible lesion, especially if it is equal to or thicker than 4mm or the joint space. In some instances, prognosis cannot be reliably determined requiring the need for follow-up imaging. PMID:21704251

Vande Berg, B; Lecouvet, F; Koutaïssoff, S; Simoni, P; Maldague, B; Malghem, J

2011-06-01

93

Leishmania donovani bodies in bone marrow  

PubMed Central

Key Clinical Message We report a case of a 5-year-old female, resident of Afghanistan, who presented with fever and massive splenomegaly. Bone marrow revealed Leishmania donovani bodies (LD bodies) in macrophages characterized by a kinetoplast and characteristic double dot appearance. She was diagnosed as visceral leishmaniasis which is transmitted by sandflies (Phlebotomus).

Ali, Natasha; Hussain, Shabneez

2014-01-01

94

Value of Bone Marrow Imprint Smears in Early Diagnosis Of Bone Marrow Pathologies  

PubMed Central

Background: Examination of bone marrow plays a pivotal role in the practice of haematology. It can be evaluated by three ways–bone marrow aspiration (BMA), bone marrow touch imprints (BMI) and bone marrow biopsy (BMBx). Aim and Objective: To study the efficacy and reliability of BMI smears in comparison to BMA smears, in making a diagnosis of diseases involving bone marrow. Setting and Designs: This study was carried out in the Department of Pathology, Institute of Medical Sciences, Varanasi over a period of 26 months. Materials and Methods: A total number of 182 cases, with their BMA, BMI and BMBx samples (from each and every case), were evaluated and their findings even compared. Statistical Analysis: All the observations were evaluated using simple and basic statistical tool, i.e. percentage. Results: The cellularity or cell density on BMI correlated with the cellularity of BMBx in 78.6% cases, which was higher than the value observed with BMA smears (71.4%). The spreading quality was better and cytological details were better appreciated in BMI as compared to BMA. Also, the presence of lymphoglandular bodies and particles on BMI were additive diagnostic clues. All of those findings were reflected in the higher diagnostic accuracy of BMI than BMA. Conclusion: BMI should be a standard practice and be considered as an early and reliable diagnostic tool for evaluating bone marrow pathologies PMID:25584225

Das, Subhajit; Bundhun, Soobashchan

2014-01-01

95

Facilitation of allogeneic bone marrow transplantation by a T cell-specific immunotoxin containing daunomycin  

SciTech Connect

Daunomycin coupled via an acid-sensitive spacer to monoclonal Thy-1.2-specific antibody was used to purge T lymphocytes from a 1:1 mixture of murine C57BL/6J bone marrow and spleen cells prior to engraftment in fully allogeneic, irradiated BALB/c recipients. Treatment of bone marrow with the immunotoxin at a concentration used for purging had no effect on the viability of committed hematopoietic progenitor or multipotent stem cells. All of the recipients of purged bone marrow were at least 80% chimeric for donor peripheral blood cells and none developed graft-versus-host disease. Out of 50 chimeras, 49 were still alive more than 200 days posttransplantation. The chimeras were shown to be tolerant to donor tissue as tested by mixed lymphocyte reactivity, cell-mediated cytotoxicity, and skin grafting. The same tests revealed full immunocompetence of chimeras to third-party alloantigens. In vivo IgM and IgG antibody responses to sheep red blood cells were similar in magnitude in allogeneically and syngeneically reconstituted mice.

Xie, S.S.; Inazawa, M.; Sinha, N.; Sawada, S.; Vergidis, R.; Diener, E.

1987-12-01

96

Peripheral Red Blood Cell Split Chimerism as a Consequence of Intramedullary Selective Apoptosis of Recipient Red Blood Cells in a Case of Sickle Cell Disease  

PubMed Central

Allogeneic cellular gene therapy through hematopoietic stem cell transplantation is the only radical cure for congenital hemoglobinopathies like thalassemia and sickle cell anemia. Persistent mixed hematopoietic chimerism (PMC) has been described in thalassemia and sickle cell anemia. Here, we describe the clinical course of a 6-year-old girl who had received bone marrow transplant for sickle cell anemia. After the transplant, the patient showed 36% donor hematopoietic stem cells in the bone marrow, whereas in the peripheral blood there was evidence of 80% circulating donor red blood cells (RBC). The analysis of apoptosis at the Bone Marrow level suggests that Fas might contribute to the cell death of host erythroid precursors. The increase in NK cells and the regulatory T cell population observed in this patient suggests that these cells might contribute to the condition of mixed chimerism. PMID:25408852

Marziali, Marco; Isgrò, Antonella; Sodani, Pietro; Gaziev, Javid; Fraboni, Daniela; Paciaroni, Katia; Gallucci, Cristiano; Alfieri, Cecilia; Roveda, Andrea; De Angelis, Gioia; Cardarelli, Luisa; Ribersani, Michela; Andreani, Marco; Lucarelli, Guido

2014-01-01

97

MR imaging of therapy-induced changes of bone marrow  

PubMed Central

MR imaging of bone marrow infiltration by hematologic malignancies provides non-invasive assays of bone marrow cellularity and vascularity to supplement the information provided by bone marrow biopsies. This article will review the MR imaging findings of bone marrow infiltration by hematologic malignancies with special focus on treatment effects. MR imaging findings of the bone marrow after radiation therapy and chemotherapy will be described. In addition, changes in bone marrow microcirculation and metabolism after anti-angiogenesis treatment will be reviewed. Finally, new specific imaging techniques for the depiction of regulatory events that control blood vessel growth and cell proliferation will be discussed. Future developments are directed to yield comprehensive information about bone marrow structure, function and microenvironment. PMID:17021706

Henning, Tobias; Link, Thomas M.

2006-01-01

98

Red Harvester Ants  

E-print Network

. ? Horned lizards normally inhabit flat, open, dry country with little cover. Urbanization, mowing, shredding, shallow discing and other land use practices can eliminate or reduce the production of weed seeds on which harvester ants feed. Harvester ants... Entomologist, The Texas A&M University System. Red harvester ants Food sources Red harvester ant foragers collect seeds and dead insects and store them in the nests as food for the colony. The ants? mouthparts are designed for chewing. Management Red...

Drees, Bastiaan M.

2006-04-24

99

Isolation, culture, and induced multiple differentiation of Mongolian sheep bone marrow-derived mesenchymal stem cells.  

PubMed

The aim of this paper was to explore the optimal method of isolating, purifying, and proliferating Mongolian sheep bone marrow-derived mesenchymal stem cells (BMSCs) and their multiple differentiation potentialities. Bone marrow (BM) was punctured from ?1-year-old sheep, and BMSCs were harvested through gradient centrifuge and adherent cultures. Analysis of the growth of the passage 1, 5, and 10 cultures revealed an S-shaped growth curve with a population doubling time of 31.2 h. Karyotyping indicated that the chromosome number in the Mongolian sheep was 2n?=?54, comprising 26 pairs of autosomes and one pair of sex chromosomes (XY). RT-PCR demonstrated that OCT4, SOX2, and Nanog genes at passage 3 were positively expressed. The P3 BMSCs were cultured in vitro under inductive environments and induced into adipocytes, osteoblasts, chondrocytes, neural cells, and cardiomyocytes. Their differentiation properties were confirmed by histological staining, such as oil red, Alizarin red, hematoxylin-eosin, toluidine blue, and periodic acid schiff. RT-PCR showed that the specific genes to be induced were all expressed. This proves that the isolated cells are indeed the BMSCs and also provides valuable materials for somatic cell cloning and transgenic research. PMID:24399254

Liu, Zongzheng; Wang, Wei; Gao, Jinfang; Zhou, Huanmin; Zhang, Yanru

2014-05-01

100

Genotoxicity of silver and titanium dioxide nanoparticles in bone marrow cells of rats in vivo.  

PubMed

Although nanomaterials have the potential to improve human life, their sideline effects on human health seem to be inevitable and still remain unknown. This study aimed to investigate the cytotoxicity and genotoxicity of titanium dioxide (TiO2) and silver (Ag) nanoparticles (NPs) at different doses and particle sizes to bone marrow cells. Both types of nanoparticles were chosen due to their wide applications of them in consumer products. Rats were injected intravenously with a single dose of 5 or 10 mg/kg bw of 20 nm AgNPs or with 5 mg/kg bw 200 nm AgNPs or with 5 mg/kg bw 21 nm TiO2NPs. The samples were taken at 24 h, 1 week and 4 weeks following the exposure. Micronucleus test and the Comet assay were used to detect DNA damage. Neither AgNPs nor TiO2NPs caused cytotoxicity to bone marrow red and white cells. The polychromatic erythrocytes are the main target of both nanoparticles. A single exposure to AgNPs induced significantly enhanced frequency of micronuclei not only at 24 h after exposure, but also 1 and 4 weeks later, whereas single exposure to TiO2NPs showed positive effect at 24 h only. Negative responses were shown in reticulocytes (micronuclei) and in leukocytes (Comet assay) of bone marrow. Results indicated that different bone marrow cells display different susceptibility toward genotoxicity mediated by both investigated nanoparticles. The use of materials containing nanoparticles and the potential health implication of them should be monitored. PMID:24321264

Dobrzy?ska, Ma?gorzata M; Gajowik, Aneta; Radzikowska, Joanna; Lankoff, Anna; Dušinská, Maria; Kruszewski, Marcin

2014-01-01

101

Pharmacokinetics and red cell utilization of 52Fe/59Fe-labelled iron polymaltose in anaemic patients using positron emission tomography.  

PubMed

Parenteral iron-polysaccharide complexes are increasingly applied. The pharmacokinetics of iron sucrose have been assessed by our group using positron emission tomography (PET). A single intravenous injection of 100 mg iron as iron (III) hydroxide-polymaltose complex, labelled with a tracer in the form of 52Fe/59Fe, was similarly assessed in six patients using PET for about 8 h. Red cell utilization was followed for 4 weeks. Iron polymaltose was similarly distributed to the liver, spleen and bone marrow. However, a larger proportion of this complex was rapidly distributed to the bone marrow. The shorter equilibration phase for the liver, about 25 min, indicates the minimal role of the liver for direct distribution. Splenic uptake also reflected the reticuloendothelial handling of this complex. Red cell utilization ranged from 61% to 99%. Despite the relatively higher uptake by the bone marrow, there was no saturation of marrow transport systems at this dose level. In conclusion, high red cell utilization of iron polymaltose occurred in anaemic patients. The major portion of the injected dose was rapidly distributed to the bone marrow. In addition, the reticuloendothelial uptake of this complex may reflect the safety of polysaccharide complexes. Non-saturation of transport systems to the bone marrow indicated the presence of a large interstitial transport pool, which might possibly be transferrin. PMID:12614222

Beshara, Soheir; Sörensen, Jens; Lubberink, Mark; Tolmachev, Vladimir; Långström, Bengt; Antoni, Gunnar; Danielson, Bo G; Lundqvist, Hans

2003-03-01

102

The bone marrow lesion in osteoarthritis  

Microsoft Academic Search

Osteoarthritis (OA) is considered a multifactorial disease whose development and progression may include several structural\\u000a abnormalities aside from cartilage destruction. Bone marrow lesions (BMLs) have been reported to be associated with OA pathology,\\u000a and several studies have advocated its close connection to the severity of joint structural alterations and pain, the main\\u000a OA clinical manifestation. Hence, BMLs may not only

Massoud Daheshia; Jian Q. Yao

2011-01-01

103

Processing of Human Cord Blood by Three Different Procedures for Red Blood Cell Depletion and Mononuclear Cell Recovery  

Microsoft Academic Search

Background and Objectives: Human cord blood (CB) is an important source of stem cells which may be used for hematopoietic reconstitution as an alternative to bone marrow transplantation. Banking of CB would be accomplished by removing red blood cells (RBC) and plasma from CB collections. Our aim was to compare three different procedures for CB processing. Materials and Methods: Poligeline,

Paolo Perutelli; Silvia Catellani; Lucia Scarso; Giorgio Dini

1999-01-01

104

Cobb's Red Cabbage Indicator.  

ERIC Educational Resources Information Center

Describes the use of an indicator made from the pigment in red cabbage. Cabbage is grated then soaked in water. When the water is a strong red, the cabbage is strained out. The cabbage-juice indicator is then used to test for acids and bases. Includes a list of good foods to test for acidity and alkalinity. (PVD)

Cobb, Vicki

1998-01-01

105

Red Clover Breeding Progress  

Technology Transfer Automated Retrieval System (TEKTRAN)

Red clover (Trifolium pratense L.) is an important forage legume grown on approximately 4 million hectares worldwide. It has a long and varied history in agriculture. Active breeding efforts began at the end of the 19th century. Since this time significant improvement in red clover cultivar for a...

106

A clinical overview of bone marrow edema.  

PubMed

Bone marrow edema (BME) is a descriptive term which identifies a specific magnetic resonance imaging (MRI) pattern that can be observed in a number of clinical entities, which are often characterized by pain as their main symptom, but show significant differences in terms of histopathological findings, causal mechanisms and prognosis. Bone marrow lesions in the subchondral bone of subjects with knee osteoarthritis (OA) seem to be associated with pain and progression of cartilage damage over time. Some histopathological studies of advanced OA have shown a prevalent fibrosis and bone marrow necrosis. BME of the subchondral bone in rheumatoid arthritis is associated with an infiltrate of inflammatory cells and osteoclasts and has a predictive value of further development of erosions. In spondyloarthritis, BME of the sacroiliac joints identifies an active sacroiliitis and is associated with histological inflammation and radiographic progression, whereas the relationship between BME lesions of the spine and syndesmophyte development is still controversial. BME syndromes (BMES), such as transient osteoporosis of the hip, regional migratory osteoporosis, and transient post-traumatic BMES, are characterized by a BME pattern on MRI and a self-limiting course. The potential evolution of BMES toward osteonecrosis is still controversial. PMID:25069499

Manara, M; Varenna, M

2014-01-01

107

In vitro proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells from ovariectomized rats.  

PubMed

Bone marrow-derived mesenchymal stem cells (BMMSCs) from the patients suffering from age-related osteoporosis were found to have numerous degeneration, such as decreased growth rate, impaired capacity of differentiating into local tissue, and repressed telomerase activity. However, it is not clear whether post-menopausal osteoporotic bone is either subject to such decline in cellular function. In the present study, bone marrow cells were harvested from ovariectomized (OVX) and Sham rats and cultured in vitro at 3 months post-surgery. MTT assay indicated that the proliferation potential of (OVX)BMMSCs was always higher than that of (Sham)BMMSCs, no matter cultured in basic, osteoblastic or adipogenic medium. Alkaline phosphatase activity assay, Alizarin red S staining, Oil red O staining and real-time RT-PCR analysis further demonstrated that bilateral ovariectomization positively influenced the osteoblastic and adipogenic differentiation potential of BMMSCs, this action may be partly mediated through up-regulation of osteoblastic special markers core binding factor a1, collagen type I and low-density lipoprotein receptor-related protein 5, as well as adipogenic special markers peroxisome proliferators activated receptor gamma, CCAAT/enhancer binding protein alpha and adipocyte lipid-binding protein 2. These results may hold great promise for using post-menopausal osteoporotic bone as an attractive autologous marrow source for tissue engineering and cell-based therapies. PMID:25257163

Gao, Ying; Jiao, Yanjun; Nie, Wei; Lian, Bo; Wang, Binquan

2014-12-01

108

International Federation of Red Cross and Red Crescent Societies  

MedlinePLUS

... Abdul Rahman Attar, President of the Syrian Arab Red Crescent, welcomed Mr. Elhadj As Sy, ... Featured video ... of fraudulent web sites which are using the Red Cross Red Crescent emblems to request donations for ...

109

69. Red Butte-Red Fir Ridge (Shasta Red Fir) (Imper 1988b, Cheng 1996d)  

E-print Network

69. Red Butte-Red Fir Ridge (Shasta Red Fir) (Imper 1988b, Cheng 1996d) Location This established is SE., although some SW. and NE. exposures exist. The W. part is dominated by Red Butte, a recent. Parts of the Mud Creek canyon are very steep, as are the upper slopes of Red Butte. Rocks are entirely

Standiford, Richard B.

110

Bone marrow transfusions in previously irradiated, hematologically normal syngeneic mice  

SciTech Connect

Transfusion of syngeneic marrow into normal, nonirradiated recipients results only in minimal proliferation of donor cells. However, irradiated recipients, restored to hematologic normalcy by an initial marrow transfusion, subsequently sustain proliferation which replaces approximately 10% of endogenous marrow after a single transfusion of 4 x 10/sup 7/ marrow cells of the same strain as the host. Cells from histoincompatible donors proliferate only rarely or minimally in the marrows of these irradiated, but hematologically normal recipients without reirradiation. Syngeneic male donor cells proliferate in irradiated and restored female mice, while female donor cells fail to proliferate in the marrow of syngeneic male recipients. A possible explanation is that transfused female cells respond immunologically to the abundant H-Y antigen in the male environment and are eliminated as a result.

Brecher, G. (Univ. of California, Berkeley); Lawce, H.; Tjio, J.H.

1981-03-01

111

Post-Red Supergiants  

E-print Network

The yellow hypergiants are found in a stage between the massive Red Supergiants and the Wolf-Rayet stars. This review addresses current issues concerning the evolution of massive stars, concentrating on the transitional post-Red Supergiant phase. Few yellow hypergiants are known and even fewer show direct evidence for having evolved off the Red Supergiant branch. Indeed, only two such rare objects with clear evidence for having gone through of a previous mass losing phase are known, IRC +10420 and HD 179821. We will review their properties and present recent results employing near-infrared interferometry, integral field spectroscopy and polarimetry. Finally, their real-time evolution is discussed.

Rene Oudmaijer; Ben Davies; Willem-Jan de Wit; Mitesh Patel

2008-01-15

112

Red blood cell production  

MedlinePLUS Videos and Cool Tools

... or another. Red blood cells are an important element of blood. Their job is to transport oxygen ... hemocytoblasts give rise to all of the formed elements in blood. If a hemocytoblast commits to becoming ...

113

Egypt and Red Sea  

NASA Technical Reports Server (NTRS)

A panaramic view of eastern Egypt, The Red Sea and Saudi Arabia beyond (24.0N, 33.0E). In this desert country, where water is life, the high Aswan Dam and the impounded waters of the Nile River in the foreground assure water availability into the next century. The Red Sea beyond, part of the Suez Canal seaway, serves as a commercial link to the world and separates Egypt from Saudi Arabia.

1982-01-01

114

Red clover with moth  

NSDL National Science Digital Library

Red clover plants are producers. This means that they make their own energy and food and do not need to eat other organisms to gain energy and live. Red clover use the sun, water, and carbon dioxide to go through photosynthesis and make their own energy to grow, bloom, and reproduce. The moth drinking nectar from the bloom is a consumer because it relies on other organisms for energy.

Sage Ross (None;)

2007-09-23

115

2012 Special symposium on Red tides Red-tide world  

E-print Network

2012 Special symposium on Red tides Red-tide world Date: October 26 (Friday) 2012 09 on Red tides Time Topic Speaker Organization 8:50-9:10 Registration 9:10-9:20 Welcome 9:20-10:00 Forecasting the New England red tide : Current status, and ongoing efforts Don Anderson Woods Hole Oceanogr. i

Jeong, Hae Jin

116

Pitfalls in bone marrow pathology: avoiding errors in bone marrow trephine biopsy diagnosis  

Microsoft Academic Search

Avoiding errors in the histological interpretation of bone marrow trephine biopsy specimens requires an unprecedented degree of collaboration between histopathologists, haematologists, specimen requesters, specimen takers, laboratory technical staff and other scientific staff. A specimen of good quality, with full, relevant clinical information is the essential starting point. This must then be processed optimally and investigated appropriately, involving immunophenotyping and molecular

Bridget S Wilkins

2011-01-01

117

Bone marrow transplantation in dogs after radio-ablation with a new Ho-166 amino phosphonic acid bone-seeking agent (DOTMP).  

PubMed

beta-emitting 166Ho (t1/2 = 26.78 hours, E(beta)max = 1.8 MeV) complexed with the phosphonic acid chelator, 1,4,7,10 tetraazacyclododecane-1,4,7,10-tetra(methylene phosphonic acid) (DOTMP) at a ligand-to-metal ratio of 1.5:1 binds to bone. This radioactive complex is a marrow-ablating radiopharmaceutical that appears useful for preparation of bone marrow (BM) transplant recipients without the morbidity usually associated with total body irradiation preparatory regimens. We have found with seven splenectomized young adult beagle dogs that a 166Ho radiopharmaceutical dosage of 370 MBq/kg body weight provides an initial skeletal radioactivity burden of at least 1.5 GBq/kg skeleton and results in complete ablation of hematopoietic marrow cell populations within 7 days. The beta particle flux distribution in BM-forming skeletal tissue is not uniform. Red marrow radiation doses varied from 30 to 115 Gy as estimated by direct radioassay and autoradiographic analyses of both bone biopsies and postmortem samples; the median value of 61 Gy agreed with our theoretical expectations. In vivo radioactivity distribution was evaluated with nuclear imaging methods. Apparently, normal hematopoiesis was restored in three dogs with autologous BM transplants performed 5 to 6 days after administration of the marrow ablative radiopharmaceutical, 166Ho-DOTMP. BM biopsies at 7 to 10 months posttransplantation indicate continued normal hematopoietic activity. PMID:8324232

Parks, N J; Kawakami, T G; Avila, M J; White, R; Cain, G R; Raaka, S D; Hornoff, W; Fisher, P; Moore, P; Seibert, J A

1993-07-01

118

A case of parvovirus B19-induced pure red cell aplasia in a child following heart transplant.  

PubMed

We describe a case of an 11-year-old boy who underwent orthotopic heart transplant for dilated cardiomyopathy. He developed a normocytic, normochromic anaemia with a low reticulocyte count 1 month after transplant. A bone marrow biopsy was performed, which showed a mildly hypocellular bone marrow with few red blood cell precursors with giant pro-erythroblasts indicative of a pure red cell aplasia. Parvovirus B19 polymerase chain reaction in the blood was positive 2 months after transplant. Intravenous immunoglobulin administration resulted in a resolution of the anaemia over several months. Unexplained pure red cell aplasia in immunosuppressed patients should alert one to the possibility of parvovirus B19 infection. PMID:24555951

Kelleher, Eoin; McMahon, Corrina; McMahon, Colin J

2015-02-01

119

Bone Marrow Transplantation and Peripheral Blood Stem Cell Transplantation: Questions and Answers  

MedlinePLUS

... procedure for obtaining bone marrow, which is called “harvesting,” is similar for all three types of BMTs ( ... to draw the marrow out of the bone. Harvesting the marrow takes about an hour. The harvested ...

120

Targeted Pathologic Evaluation of Bone Marrow Donors Identifies Previously Undiagnosed Marrow Abnormalities  

PubMed Central

Potential bone marrow donors are screened to ensure the safety of both the donor and recipient. At our institution, potential donors with abnormal peripheral blood cell counts, a personal history of malignancy, or age >60 years are evaluated to ensure that they are viable candidates for donation. Evaluation of the marrow includes morphologic, flow cytometric and cytogenetic studies. 122 potential donors were screened between the years of 2001–2011, encompassing approximately 10% of all donors. The median age of the screened potential donors was 59 years, and included 59 men and 63 women. The donors were screened because of age >60 years old (33), anemia (22), cytopenias other than anemia (27), elevated peripheral blood counts without a concurrent cytopenia (20), elevated peripheral blood counts with a concurrent cytopenia (10), history of malignancy (4), abnormal peripheral blood differential (3), prior graft failure (1), history of treatment with chemotherapy (1), and body habitus (1). Marrow abnormalities were detected in 9% (11/122) of donors. These donors were screened because of anemia (5/22; 23%), age >60 years (2/33; 6%), history of malignancy (2/4; 50%), elevated peripheral blood counts (1/20; 5%), and body habitus (1/1; 100%). Abnormalities included plasma cell dyscrasia (3), abnormal marrow cellularity (3), clonal cytogenetic abnormalities (2), low-grade myelodysplastic syndrome (1), a mutated JAK2 V617F allele (1), and monoclonal B-cell lymphocytosis (1). Our experience indicates that extended screening of potential donors identifies a significant number of donors with previously undiagnosed marrow abnormalities. PMID:23769818

Tilson, MP; Jones, RJ; Sexauer, A; Griffin, CA; Morsberger, LA; Batista, DAS; Small, D; Burns, KH; Gocke, CD; Vuica-Ross, M; Borowitz, MJ; Duffield, AS

2013-01-01

121

Myelodysplastic patients with raised percentage of hypochromic red cells have evidence of functional iron deficiency  

Microsoft Academic Search

Raised percentage hypochromic red cells (%HRC) were detected at diagnosis in 10 of 34 consecutive patients with low-risk myelodysplastic syndrome (MDS) [refractory anemia (RA) (4\\/26) and RA with ring sideroblasts (6\\/8)], all of whom had normal or increased serum ferritin and bone marrow iron stores. Elevated %HRC has persisted in all 10 cases and subsequently developed in another RA patient

P. T. Murphy; J. P. Quinn; D. O’Donghaile; R. Swords; J. R. O’Donnell

2006-01-01

122

Avascular necrosis and bone marrow edema syndrome.  

PubMed

MR imaging is the most accurate modality for the diagnosis of AVN, bone marrow edema syndrome, and femoral head insufficiency fractures. When the particular demographics of the patient are considered, the specificity of this examination is high. The reported success rates of joint-sparing operative intervention are variable. When compared with conservative management, the outcome of joint-sparing operative intervention in patients who have early (stage I, II) AVN lesions is improved. This is impetus for screening programs for patients who are known to be at high risk for AVN, such as patients who have undergone renal transplants and others who are on long-term, high-dose corticosteroid treatment. There is strong evidence that bone marrow edema syndrome (TO) is a distinct entity with demonstrable unique histopathology and well-defined demographics. Increased spatial resolution revealed typical subchondral findings of AVN in lesions that were once believed to represent irreversible TO. SIF of the femoral head is a new concept with a seemingly distinct population and with clinical presentation and imaging characteristics that should permit its differentiation from AVN. PMID:15049532

Watson, Rohan M; Roach, Neil A; Dalinka, Murray K

2004-01-01

123

Controlling the Bone Marrow Dynamics in Cancer Chemotherapy  

E-print Network

Professor Award 1 #12;find optimal strategies for chemotherapy treatments of the cancer, where Controlling the Bone Marrow Dynamics in Cancer Chemotherapy Urszula Ledzewicz1 and Heinz Sch In the paper a mathematical model for the growth of the bone marrow under cell-cycle specific cancer

Ledzewicz, Urszula

124

Bone marrow mesenchymal stem cells are abnormal in multiple myeloma  

Microsoft Academic Search

Recent literature suggested that cells of the microenvironment of tumors could be abnormal as well. To address this hypothesis in multiple myeloma (MM), we studied bone marrow mesenchymal stem cells (BMMSCs), the only long-lived cells of the bone marrow microenvironment, by gene expression profiling and phenotypic and functional studies in three groups of individuals: patients with MM, patients with monoclonal

J Corre; K Mahtouk; M Attal; M Gadelorge; A Huynh; S Fleury-Cappellesso; C Danho; P Laharrague; B Klein; T Rème; P Bourin

2007-01-01

125

Chronic Disseminated Histoplasmosis Bone Marrow Involvement in an Immunocompetent Patient  

PubMed Central

Histoplasmosis normally do not affect immunocompetent individuals. Prolonged exposure may cause chronic disseminated histoplasmosis. Elderly male patient presented with fever, hematuria and pain in right hypochondrium. He had pallor, fever and mild hepatosplenomegaly. Investigations revealed anemia and thrombocytopenia. Giemsa stained bone marrow aspirate showed yeast-like cells, suggestive of Histoplasma capsulatum. PAS stained bone marrow aspirate and biopsy confirmed the diagnosis. PMID:21629639

Tangri, Rajiv; Verma, Neena; Gautam, Dheeraj

2010-01-01

126

Painless transient bone marrow edema syndrome in a pediatric patient.  

PubMed

Transient regional migratory osteoporosis, considered to be part of the spectrum of bone marrow edema syndrome, is a rare condition with an unknown etiology. Patients usually present with lower extremity pain, most commonly in the 4th-5th decades of life. We describe a 15-year-old male patient with type 1 Gaucher disease who presented with transient bone marrow edema syndrome with features most closely resembling regional migratory osteoporosis. The patient presented with bone marrow edema of the lateral tibial epiphysis of his right knee that was incidentally seen on routine surveillance MRI that was performed as protocol for patients with type 1 Gaucher disease on enzyme replacement therapy. At this time, the patient had no pain and physical examination was normal. Follow-up MRI of the right knee 4 months afterward showed complete resolution of the signal abnormality in the right tibial epiphysis, and repeat study 8 months later displayed a new focus of painless migratory edema of the medial tibial epiphysis of the same knee. These changes completely resolved as well. Marrow signal abnormalities in children with Gaucher disease can have a broad differential, including infection, marrow infiltration, trauma, osteonecrosis, and bone marrow edema syndrome, amongst others. Correct diagnosis of bone marrow edema syndrome is critical, as this disease process most often resolves on conservative measures. The unusual presentation of transient bone marrow edema syndrome with regional migratory osteoporosis features in a young patient with Gaucher disease is described. PMID:24893724

Joshi, Vivek; Hermann, George; Balwani, Manisha; Simpson, William L

2014-11-01

127

Bone Marrow Stromal Cells Generate Muscle Cells and  

E-print Network

12/14/09 1 Bone Marrow Stromal Cells Generate Muscle Cells and Repair Muscle Degeneration By Stephanie Aracena & Roynan Krebs Bone Marrow Stromal Cells What are they? Where are they located? Why not stem cells? Small populations Ethical reasons Histocompatibility Difficult to get #12;12/14/09 2

Devoto, Stephen H.

128

The bone marrow microenvironment and leukemia: biology and therapeutic targeting.  

PubMed

Multiple studies have demonstrated that interaction with the bone marrow stromal microenvironment contributes to the survival of leukemia cells. One explanation for this phenomenon is the interaction between the cell surface receptors CXCR4 and CXCL12. Through CXCL12/CXCR4-mediated chemotaxis, leukemia cells migrate to microscopic niches within the bone marrow, which leads to increased proliferation and survival. Several studies have suggested that increased CXCR4 expression may portend a poor prognosis in various types of leukemia, possibly due to increased protection of leukemia cells by bone marrow stroma. A potential therapeutic strategy to overcome this stromal-mediated survival advantage is to target CXCR4. Inhibition of CXCR4 may allow leukemia cells to be released from bone marrow niches that confer resistance to chemotherapy and negate the survival benefit imparted by bone marrow stroma. PMID:21668393

Sison, Edward Allan R; Brown, Patrick

2011-06-01

129

The bone marrow microenvironment and leukemia: biology and therapeutic targeting  

PubMed Central

Multiple studies have demonstrated that interaction with the bone marrow stromal microenvironment contributes to the survival of leukemia cells. One explanation for this phenomenon is the interaction between the cell surface receptors CXCR4 and CXCL12. Through CXCL12/CXCR4-mediated chemotaxis, leukemia cells migrate to microscopic niches within the bone marrow, which leads to increased proliferation and survival. Several studies have suggested that increased CXCR4 expression may portend a poor prognosis in various types of leukemia, possibly due to increased protection of leukemia cells by bone marrow stroma. A potential therapeutic strategy to overcome this stromal-mediated survival advantage is to target CXCR4. Inhibition of CXCR4 may allow leukemia cells to be released from bone marrow niches that confer resistance to chemotherapy and negate the survival benefit imparted by bone marrow stroma. PMID:21668393

Sison, Edward Allan R; Brown, Patrick

2011-01-01

130

Red Cabbage Chemistry  

NSDL National Science Digital Library

Students take advantage of the natural ability of red cabbage juice to perform as a pH indicator to test the pH of seven common household liquids. Then they evaluate the accuracy of the red cabbage indicator, by testing the pH of the liquids using an engineer-designed tool, pH indicator strips. Like environmental engineers working on water remediation or water treatment projects, understanding the chemical properties (including pH) of contaminants is important for safeguarding the health of environmental water sources and systems.

GK-12 Program,

131

Bone marrow replacement in the treatment of hemolytic disease in mice  

SciTech Connect

Bone marrow replacement therapy following whole-body x- or gamma-irradiation has until now proven to be of limited value in the treatment of individuals with hemolytic disease. The large doses of radiation required for destruction of defective erythropoietic tissues coupled with their resultant high mortality appears to limit its usefulness. Techniques have been developed by the authors to limit the extent of exposure and to improve survival following irradiation. These techniques include shielding of all parts of the body except the hind limbs, prophylactic use of antibiotics, and preparatory blood transfusion to suppress the development of indigenous defective erythrocytes. Using these combined techniques we were able to establish high rates of survival, successful engraftment, and long-term clinical improvement in mice with several hemolytic disorders emanating from hereditary defects in spectrin production and incorporation. Evidence is presented indicating that complete bone marrow replacement occurs even in nonirradiated portions of the erythron and that only donor type red blood cells appear in the circulation.

Bernstein, S.E.; Deveau, S.A. (Jackson Lab., Bar Harbor, ME (USA))

1989-11-01

132

Activation of Cannabinoid Receptor 2 Enhances Osteogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells  

PubMed Central

Bone marrow derived mesenchymal stem cells (BM-MSCs) are considered as the most promising cells source for bone engineering. Cannabinoid (CB) receptors play important roles in bone mass turnover. The aim of this study is to test if activation of CB2 receptor by chemical agonist could enhance the osteogenic differentiation and mineralization in bone BM-MSCs. Alkaline phosphatase (ALP) activity staining and real time PCR were performed to test the osteogenic differentiation. Alizarin red staining was carried out to examine the mineralization. Small interference RNA (siRNA) was used to study the role of CB2 receptor in osteogenic differentiation. Results showed activation of CB2 receptor increased ALP activity, promoted expression of osteogenic genes, and enhanced deposition of calcium in extracellular matrix. Knockdown of CB2 receptor by siRNA inhibited ALP activity and mineralization. Results of immunofluorescent staining showed that phosphorylation of p38 MAP kinase is reduced by knocking down of CB2 receptor. Finally, bone marrow samples demonstrated that expression of CB2 receptor is much lower in osteoporotic patients than in healthy donors. Taken together, data from this study suggested that activation of CB2 receptor plays important role in osteogenic differentiation of BM-MSCs. Lack of CB2 receptor may be related to osteoporosis.

Sun, Yong-Xin; Xu, Ai-Hua; Yang, Yang; Zhang, Jia-Xing; Yu, Ai-Wen

2015-01-01

133

The bone marrow in nutritional deficiencies.  

PubMed

The overwhelming majority of nutritional deficiencies that affect the bone marrow and blood are due to the lack of vitamin B12, folic acid, or iron or combinations thereof. The two vitamins are closely related in DNA synthesis, whereas iron is the most abundant heavy metal in the body and is chiefly utilized for hemoglobin synthesis. Concomitant conditions of vitamin B12 and/or folate deficiency along with iron deficiency are not infrequent, and one type of anemia may mask the other. It is important to establish the correct diagnoses, as therapy directed at the wrong deficiency may hide the real deficiency with disastrous results. Specific diagnostic tests are now available to determine definitive diagnoses, and specific therapy is readily available to restore and maintain a normal nutrient status. PMID:3065318

Krause, J R

1988-12-01

134

The Inherited Bone Marrow Failure Syndromes  

PubMed Central

In spite of the rarity of inherited bone marrow failure syndromes (IBMFS), they represent diseases for which the molecular pathogenesis may be elucidated. Their study and presentation of the details of their molecular biology and biochemistry is warranted not only for appropriate diagnosis and management of afflicted patients but also because they lend clues to the normal physiology of the normal hematopoiesis and, in many cases, mechanisms of carcinogenesis. Several themes have emerged within each subsection of IBMFS, including the ribosomopathies that entail both ribosome assembly as well as ribosomal RNA processing. The Fanconi anemia (FA) pathway itself has become interdigitated with the familial breast cancer syndromes. The sections that follow present a more detailed analysis of the diseases that account for the majority of IBMFS diagnoses. PMID:24237972

Chirnomas, S. Deborah; Kupfer, Gary M

2013-01-01

135

Hematopathology: SC14-1 CHALLENGES IN BONE MARROW PATHOLOGY.  

PubMed

Bone marrow diagnosis presents unique challenges: distinction between benign and neoplastic conditions may be subtle and the pathologist must effectively incorporate information from several morphologic modalities (trephine biopsy, aspirate smear, peripheral blood smear) as well as a myriad of ancillary testing results. In this Short Course, several difficult diagnostic scenarios will be explored using actual clinical cases that illustrate key points in the differential diagnosis of bone marrow diseases. These will include hypocellular bone marrow (in which distinction between hypoplastic myelodysplastic syndrome and aplastic anemia may be difficult), fibrotic bone marrow, and marrow lymphoid infiltrates, with discussion of both B-cell and T-cell lymphomas. Hypercellular bone marrow raises distinct differential diagnoses depending on whether the patient is cytopenic or presents with elevated counts, and can reflect a myeloid neoplasm or various reactive conditions in either situation. An accurate diagnosis relies on understanding the spectrum of diseases that can produce specific patterns in the bone marrow and awareness of clues that help distinguish among the differential diagnostic possibilities. Depending on the clinical context, the pathology may variably weigh morphology, clinical information, and ancillary test results in arriving at the final diagnosis. PMID:25188096

Hasserjian, Robert P; Wang, Sa A

2014-10-01

136

In vitro analysis of mesenchymal stem cells derived from human teeth and bone marrow.  

PubMed

Mesenchymal stem cells derived from human teeth and bone marrow have been characterized by many research groups, but demonstrate inconsistent cellular phenotypes or functions, partly because of differences in culture methodology. Therefore, our aims were to resolve these inconsistencies and discuss the potential uses of these cells in research/clinical applications. We isolated and characterized dental stem cells (DSCs) from the dental pulp, periodontal ligament, apical papilla (APSCs) and dental follicle (DFSCs) of mature and immature teeth, along with bone marrow-derived stem cells (BMSCs) from the iliac crest. We compared the clonogenic and proliferative potentials of these cells in terms of colony-forming efficiency, proliferation potential, population doubling time and cell cycle. All DSCs, particularly APSCs and DFSCs, possessed greater proliferative potential than BMSCs. All stem cells expressed typical mesenchymal and embryonic markers, and developed alizarin red-positive mineralization nodules and Oil red O-positive lipid droplets when cultured in osteogenic and adipogenic media, respectively. Immunocytochemistry revealed that all stem cells developed neuronal markers when cultured in a control medium without neural inductive supplements. After 7 days of neurogenic culture, the differentiated cells showed a transition from fibroblast-like to neuron-like cell bodies with long processes, suggesting that the stem cells differentiated into mature neurons. Karyotyping confirmed that the stem cells maintained a normal karyotype and were chromosomally stable. Our results provide new insights into the physiological properties of stem cells with a normal karyotype and indicate that DSCs are appropriate for basic research and clinical applications. PMID:22772774

Tamaki, Yuichi; Nakahara, Taka; Ishikawa, Hiroshi; Sato, Soh

2013-07-01

137

Florida's Red Tide Infestation  

NSDL National Science Digital Library

This CBS news article reports a toxic algae bloom that spread along the Florida's west coast in 2001, threatening the wildlife of Tampa Bay's estuary. The article briefly discusses the cause of the red tide and its affect on the shellfish industry.

News, Cbs

138

Human Red Cell Peptidases  

Microsoft Academic Search

A new method for characterizing peptidases in terms of electrophoretic behaviour in starch gel and of substrate specificity is described. A survey of red cells from a large number of people has revealed genetically determined variants of two of these enzymes. Separate loci appear to be involved.

W. H. P. Lewis; Harry Harris

1967-01-01

139

SRED: Stabilized RED  

Microsoft Academic Search

This paper describes a mechanism we call "SRED" (Stabilized Random Early Drop). Like RED (Random Early Detection) SRED pre-emptively discards packets with a load-dependent probability when a buffer in a router in the Internet or an Intranet seems congested. SRED has an additional feature that over a wide range of load levels helps it stabilize its buffer occupation at a

Teunis J. Ott; T. V. Lakshman; Larry H. Wong

1999-01-01

140

Red and Black Game  

NSDL National Science Digital Library

This resource consists of a Java applet and expository text. The applet allows the student to play the game of red and black, by betting on a sequence of Bernoulli trials until either a target is reached or the player is ruined. The target, initial fortune, and trial win probability can be varied.

Siegrist, Kyle

141

Red sea drillings.  

PubMed

Recent drilling in the Red Sea has shown that much of the basin is underlain by evaporites of a similar age to that of evaporites found in the Mediterranean Sea. These evaporites and their structural positions indicate that other brine areas are present-and, indeed, several others have been discovered. PMID:17843766

Ross, D A; Whitmarsh, R B; Ali, S A; Boudreaux, J E; Coleman, R; Fleisher, R L; Girdler, R; Manheim, F; Matter, A; Nigrini, C; Stoffers, P; Supko, P R

1973-01-26

142

Engraftment of allogeneic bone marrow without graft-versus-host disease in mongrel dogs using total lymphoid irradiation  

SciTech Connect

We achieved long-term engraftment of unmatched bone marrow (BM) in dogs without graft-versus-host disease (GVHD) using a regimen of total lymphoid irradiation (TLI) which could be applied clinically. Twelve normal adult mongrel dogs were given TLI in 18 fractions of 100 rad each (total dose, 1800 rad) over 4 weeks to mantle and abdominal fields in continuity. Nine of the 12 were transfused with one or two random donor whole blood transfusions during the irradiation regimen to determine the risk of sensitization after the onset of immunosuppression. A mean (+- SD) of 0.71 +- 0.54 x 10/sup 9/ BM cells/kg of recipient body weight from unrelated sex-mismatched donors was infused within 24 h of the 18th irradiation fraction. Engraftment was assessed by demonstration of donor-type sex chromosomes in spontaneous metaphase spreads of recipient marrow aspirates, and by the appearance of donor-type red blood cells antigens (DEA) in the recipients' blood. Three untransfused and nine transfused recipients were shown to be stable mixed BM chimeras during a followup period of 2 to 11 months after transplantation. Blood transfusion during TLI did not result in graft rejection. We observed no clinical signs of acute or chronic GVHD. TLI has minimal toxicity when compared with conditioning regimens currently used in BM transplantation for aplastic anemia. Potential advantages of the TLI regimen include the opportunity to use unmatched marrow donors and protection from GVHD.

Gottlieb, M.; Strober, S.; Hoppe, R.T.; Grumet, F.C.; Kaplan, H.S.

1980-06-01

143

[Processing of ceramiclike xenogeneic bone and experimental study of its bone formation from composite graft combined with bone marrow].  

PubMed

Ceramiclike xenogeneic bone (CXB) was obtained from the fresh bone of pig ribs being treated by physical and chemical methods to deprive of its organic substance. The CXB possessed the same natural porous network system as that of the human. The CXB was cultured with the bone marrow stromal cells of rabit. When the marrow cells had integrated with the CXB, thus a new material was obtained. (CXB-BM), and was implanted sacro-spinal muscle of rabbit. The specimens were observed under phase microscope, light microscope and electronic scanning microscope. The results showed that: at the 2nd week after the implantation of CBX-BM composite material there began the new bone formation, and the rate of bone formation was increased with time. There was evident new bone formation after 24 weeks. The process of the new bone formation were quite similar to the composite graft of HAP red autogenous and marrow, but the former degraded faster and formed typical cancellous structure earlier. There was no new bone formation when CXB was implanted alone in the control. Both the mechanism of osteogenetic potential and its clinical application were discussed. PMID:10374603

Li, Y; Zeng, C; Wang, H

1998-03-01

144

Bone marrow stromal cell assays – in vitro and in vivo  

PubMed Central

Summary Populations of bone marrow stromal cells (BMSCs, also known as bone marrow-derived “mesenchymal stem cells”) contain a a subset of cells that are able to recapitulate the formation of a bone/marrow organ (skeletal stem cells, SSCs). The biological properties of BMSC cultures are assessed by a variety of assays, both in vitro and in vivo. Application of these assays in an appropriate fashion provide a great deal of information on the role of BMSCs, and the subset of SSCs, in health and in disease. PMID:24482181

Robey, Pamela Gehron; Kuznetsov, Sergei A.; Riminucci, Mara; Bianco, Paolo

2014-01-01

145

Weather sends red tide packing  

NSDL National Science Digital Library

This Mobile Register newspaper article provides general information about the impact of a cold front on red tide in Alabama. Red tide in the area had been rising until the front passed through, killing most of the phytoplankton bloom.

Bill Finch

146

Red Blood Cell Antibody Identification  

MedlinePLUS

... Antibody ID, RBC; RBC Ab ID Formal name: Red Blood Cell Antibody Identification Related tests: Direct Antiglobulin ... None The Test Sample What is being tested? Red blood cell antibodies are proteins produced by the ...

147

Florida Red Tide Current Status  

NSDL National Science Digital Library

This Fish and Wildlife Research Institute website provides links to several Florida-based red tide sites. These sites include the current status of Florida's red tide, a photo gallery of red tide distribution maps, and regional Florida red tide statuses. The site expands further on its research and contains information on the Florida manatee, Florida panther, freshwater, geographic information systems/mapping, habitat, saltwater and wildlife. This is a nice resource for a broad overview of Florida ecosystems and natural resources.

148

Prevalence of Babesia microti-like infection in red foxes (Vulpes vulpes) from Portugal.  

PubMed

The prevalence of piroplasm (order Piroplasmida) infection was assessed in blood and bone marrow samples from 91 red foxes (Vulpes vulpes) from northern, central and southern Portugal by means of molecular methods. PCR for the 18S rRNA gene of Babesia spp. followed by sequencing revealed 63 foxes positive for the Babesia microti-like piroplasm (syn. Theileria annae) (69.2%; 95% confidence interval [CI]: 58.7-78.5%) and one fox positive for Babesia canis (1.1%; 95% CI: 0.0-6.0%). Positivity to the B. microti-like piroplasm or B. canis in 43 blood samples (83.7%) was significantly higher (p<0.001) than in 43 paired bone marrow samples (20.9%). There were no statistically significant differences in the prevalence of infection between genders (p=0.219) or age groups (<2 years vs. ? 2 years) (p=1.0). This is the first report of the B. microti-like piroplasm in foxes from Portugal as well as the first report on detection by PCR and genotyping of B. canis in a red fox worldwide. A natural cycle of the B. microti-like piroplasm is suggested in red fox populations based on the high prevalence of the protozoan. Red foxes might be a reservoir of the B. microti-like piroplasm and a source of infection to dogs. PMID:23352108

Cardoso, L; Cortes, H C E; Reis, A; Rodrigues, P; Simões, M; Lopes, A P; Vila-Viçosa, M J; Talmi-Frank, D; Eyal, O; Solano-Gallego, L; Baneth, G

2013-09-01

149

Red Rice Research and Control.  

E-print Network

Physiology, Louisiana State University, Baton Rouge. Baldwin, Ford L., University of Arkansas Cooperative Extension Service, Little Rock. Bourgeois, W. J., Department of Plant Pathology and Crop Physiology, Louisiana State University, Baton Rouge. Cox... ...................................... 10 E. A. Sonnier RED RICE CONTROL IN ALTERNATE CROPS ................................ 16 F. L. Baldwin ..# RED RICE CONTROL ..................................................lg B. A. Huey and F. L. Baldwin RED RICE HERBICIDE SCREENING TESTS...

Baker, John B.; Baldwin, Ford L.; Bourgeois, W.J.; Cox, Clodis H.; Craigmiles, Julian P.; Dishman, William D.; Eastin, E. Ford; Helpert, Charles W.; Hill, Lewis C.; Huey, Bobby A.; Klosterboer, Arlen D.; Sonnier, Earl A.

1980-01-01

150

Reasons not to deploy RED  

Microsoft Academic Search

In this paper we examine the benefits of random early detection (RED) by using a testbed made of two commercially available routers and up to 16 PCs to observe RED performance under a traffic load made of FTP transfers, together with HTTP traffic and non-responsive UDP flows. The main results we found were, first, that RED with small buffers does

Martin May; Jean Bolot; Christophe Diot; Bryan Lyles

1999-01-01

151

Red Knots at Delaware Bay  

USGS Multimedia Gallery

Red knots, an at-risk shorebird, at Delaware Bay. Red knots like to feed on horseshoe crab eggs to refuel after their marathon migrations of some 10,000 miles. Declines of horseshoe crabs and red knots seem to be related....

152

Red Hat MRG Matthew Farrellee  

E-print Network

Status Condor and Deltacloud Condor/Red Hat Collaboration Condor at 10K+ scale #12;Background · Red around Condor) · Real-time (provides pre-emption and latency determinism) · Virtualization (provides://www.redhat.com/mrg · Qpid graduated to top level Apache project · Enhanced Condor manageability · Red Hat Global

Wisconsin at Madison, University of

153

Technetium-99m antimony colloid for bone-marrow imaging  

SciTech Connect

Technetium-99m antimony colloid was prepared in our laboratory for bone-marrow imaging. Optimal production of colloid particles of size range 1 to 13 nm was achieved by the use of polyvinylpyrrolidone of mol. wt. 44,000. Electron microscopy was used to size the particles. Studies in rabbits showed exclusive concentration in the subendothelial dendritic phagocytes of the bone marrow. Pseudopods from these cells were found to traverse interendothelial junctions and concentrate colloid from the sinusoids. Imaging studies of bone marrow in rabbits showed the superiority of the Tc-99m antimony colloid over the much larger colloidal particle of Tc-99m sulfur colloid. Tissue distribution studies in the rat confirmed that bone-marrow uptake of Tc-99m antimony colloid was greater than that of Tc-99m sulfur colloid, although blood clearance was much slower.

Martindale, A.A.; Papadimitriou, J.M.; Turner, J.H.

1980-11-01

154

Who Needs a Blood and Marrow Stem Cell Transplant?  

MedlinePLUS

... Aplastic Anemia Bone Marrow Tests Sickle Cell Anemia Thalassemias Send a link to NHLBI to someone by ... and breast cancer Severe blood diseases, such as thalassemias , aplastic anemia , and sickle cell anemia Certain immune- ...

155

Bone Marrow Diseases - Multiple Languages: MedlinePlus  

MedlinePLUS

... page, please enable JavaScript. Bone Marrow Diseases - Multiple Languages French (français) Japanese (???) Korean (???) Russian (???????) ... Characters not displaying correctly on this page? See language display issues . Return to the MedlinePlus Health Information ...

156

Genetics Home Reference: Pearson marrow-pancreas syndrome  

MedlinePLUS

Pearson marrow-pancreas syndrome Mitochondrial DNA Related Condition(s) References Quick links to this topic MedlinePlus Health information Additional NIH Resources National Institutes of Health Educational resources Information ...

157

Bone scan appearances following biopsy of bone and bone marrow  

SciTech Connect

The influence of sternal marrow aspiration, iliac crest marrow aspiration, and iliac crest bone biopsy on bone scan appearances was examined. Eighteen patients were scanned a mean of 9.9 days after sternal marrow aspiration with a Salah needle. Bone scans obtained in 9 patients a mean of 10 days aftr iliac crest trephine marrow biopsy with a Jamshidi needle showed no abnormality at the biopsy site. In 18 patients with metabolic bone disease who had undergone iliac crest bone biopsy with an 8 mm needle, a scan abnormality due to the biopsy was usually present when the interval between the biopsy and the scan was 5 days to 2 months. Patients who were scanned within 3 days of iliac crest bone biopsy or more than 2 months after biopsy had normal scan appearance at the biopsy site.

McKillop, J.H.; Maharaj, D.; Boyce, B.F.; Fogelman, I.

1984-10-01

158

Clonal analysis of bone marrow and macrophage cultures  

SciTech Connect

To establish lineages that can be used to study their functional heterogeneity, the proliferation and differentiation of bone marrow derived mononuclear phagocytes and the lineages derived from them were studied. 28 references, 7 figures, 5 tables. (ACR)

Stewart, C.C.; Walker, E.B.; Johnson, C.; Little, R.

1984-01-01

159

Bone marrow osteoma of the tibia: A case report  

PubMed Central

In this study, an unusual case of osteoma is presented, whereby a bone marrow osteoma was identified in the tibia. No previous cases of bone marrow osteoma have been reported. In this case, an eight-year-old male presented with discontinuous discomfort in the right distal calf for six months. Radiological examination and computed tomography revealed a radiopaque lesion within the affected bone. A technetium-99m bone scan revealed focally increased uptake in the same region. Together, these observations prior to surgery indicated that the patient may suffer from bone disease. Subsequently, a surgical excision was performed and the biopsy specimen was identified as bone marrow osteoma. Following surgery, the symptoms were eradicated and the prognosis was positive during the 24-month follow-up period. Bone marrow osteoma should be considered when a patient suffers from discontinuous and unexplained limb discomfort. PMID:25364463

ZHOU, BEN-GEN; LIU, MEI-YUAN; LV, LI-CHUN; XIA, HONG

2014-01-01

160

Granulomas in bone marrow--a study of fourteen cases.  

PubMed

Granulomas in bone marrow are an infrequent finding and several diseases are associated with granuloma formation. Clinicopathological details of fourteen cases showing granulomas in bone marrow were studied. Fever was the commonest clinical presentation and anaemia was seen in all cases. Only one case showed epithelioid cells in the bone marrow aspirate smear while trephine biopsy was diagnostic in all cases. Granulomas were seen mainly in the paratrabecular space. Acid fast bacilli were demonstrated in five biopsies and serological test for Human Immunodeficiency virus was positive in five cases. Tuberculosis was more common in the present study as compared to others, probably due to the endemicity of tuberculosis in this region. Bone marrow biopsy is useful in the investigation of pyrexia of unknown origin as it leads to an aetiological diagnosis in most cases. PMID:16758775

Basu, Debdatta; Saravana, Rajagopal; Purushotham, Basappa; Ghotekar, Lekhraj H

2005-01-01

161

Burst-Forming Unit-Erythroid Assays (BFU-E) To Distinguish Cellular Bone Marrow Failure Disorders  

PubMed Central

Patients with cytopenias and a cellular bone marrow can be a diagnostic and a therapeutic challenge. Previous reports have suggested a role for progenitor assays as a potentially useful test for diagnosis and predicting response to therapy. Here we report the results of BFU-E assays in 48 consultative cases of single or multi-lineage cytopenias with cellular marrows. The final diagnoses included 17 patients with myelodysplastic syndrome (MDS); 9 patients with pure red cell aplasia (PRCA) [non-large granular lymphocytosis (LGL) in etiology]; 15 patients with LGL (8 of which had a single lineage cytopenia only while the other 7 had multi-lineage cytopenias); and 7 patients with cytopenias associated with systemic inflammation related to autoimmune conditions. In this cohort, nonmalignant diseases were well-distinguished from MDS by BFU-E growth. Our data suggest that low BFU-E growth (less than 10 BFU-E /105 marrow mononuclear cells) helps to exclude LGL, PRCA, or cytopenias associated with systemic inflammation as a cause of pancytopenia with a sensitivity of 96.8%, specificity of 76.5% and a predictive value of 88.2% (p=0.0001). BFU-E growth was also examined to predict response to treatment. Of the 29 patients in this cohort treated with immunosuppressive therapy across all disease groups, there was an 86% response rate with 25 responders (11 PRs and 14 CRs) and 4 non-responders. This did correlate with higher BFU-E growth. Our results suggest BFU-E assays are a useful adjunct in the diagnosis and management of cytopenias in the setting of a normocellular or hypercellular bone marrows. PMID:23660070

DeZern, Amy E.; Pu, Jeffrey; McDevitt, Michael A.; Jones, Richard J.; Brodsky, Robert A.

2013-01-01

162

Clover, red (Trifolium pratense).  

PubMed

Genetic modification of plants by the insertion of transgenes can be a powerful experimental approach to answer basic questions about gene product function. This technology can also be used to make improved crop varieties for use in the field. To apply this powerful tool to red clover, an important forage legume, a population of red clover with high potential for regeneration in tissue culture has been developed. Here we provide a detailed procedure for Agrobacterium-mediated transformation of genotypes derived from this regenerable population. We have successfully used this methodology to express ?-glucuronidase (GUS) reporter genes as well as for hairpin RNA-mediated silencing of endogenous genes for polyphenol oxidase and a transferase crucial in phaselic acid accumulation. PMID:25300845

Sullivan, Michael L; Quesenberry, Kenneth H

2015-01-01

163

The Red Brush  

NSDL National Science Digital Library

Created as part of Washington University's Digital Gateway initiative, The Red Brush project is a collection of texts in Chinese from a wide range of writings from Imperial China, by and about women writers. The materials here are available in both Chinese and English, and the project was designed to complete a narrative anthology entitled "Red Brush: Women Writers of Imperial China". The site makes the original Chinese version of over 500 poems available, along with English language translations. Visitors can use the advanced search feature here, or they can also just browse around at their leisure. The materials are divided into sixteen sections, and some of the highlights include "The full elder brother" by Ban Zhao and "The color of the water" by Hai Yin.

164

New Strategies for Allogeneic Bone Marrow Transplantation and Organ Allografts  

Microsoft Academic Search

In humans, the success rate of bone marrow transplantation (BMT) across major histocompatibility complex (MHC) barriers is not high due to: (1) graft-versus-host reaction (GvHR); (2) graft rejection, and (3) incomplete T cell recovery. In mice, GvHR can be prevented if T cell- depleted bone marrow cells (BMCs; <2% T cells) are used. Graft rejection can be prevented by either

Susumu Ikehara

1999-01-01

165

Deficient reconstitution of early progenitors after allogeneic bone marrow transplantation  

Microsoft Academic Search

Allogeneic bone marrow transplant recipients maintain normal peripheral blood counts long term, suggesting durable support from engrafted stem cells. In order to investigate late hemopoietic reconstitution at the level of committed and early progenitors (LTC-IC), we studied 64 long-term survivors at a median interval of 6 years (range: 2–20) after allogeneic bone marrow transplant. CFU-GM and BFU-E numbers did not

M Podestà; G Piaggio; F Frassoni; A Pitto; N Mordini; S Bregante; A Valeriani; A Bacigalupo

1997-01-01

166

THE GROWTH OF MOUSE BONE MARROW CELLS IN VITRO  

Microsoft Academic Search

A simple in vitro technique is described for the growth of colonies from single cell suspensions of mouse bone marrow. The system involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient types of feeder layers.Approximalely 400 colonies per

TR Bradley; D Metcalf

1966-01-01

167

Pathophysiology and Management of Inherited Bone Marrow Failure Syndromes  

PubMed Central

The inherited marrow failure syndromes are a diverse set of genetic disorders characterized by hematopoietic aplasia and cancer predisposition. The clinical phenotypes are highly variable and much broader than previously recognized. The medical management of the inherited marrow failure syndromes differs from that of acquired aplastic anemia or malignancies arising in the general population. Diagnostic workup, molecular pathogenesis, and clinical treatment are reviewed. PMID:20417588

Shimamura, Akiko; Alter, Blanche P.

2012-01-01

168

Red Tide Activities  

NSDL National Science Digital Library

This University of South Florida web site contains links to the article "Tiny Toxic Terrors: Harmful Algal Blooms" and four computer-based activities that teach students about various aspects of red tide. Activities include: "Where could they be?," "Is it a plant or animal cell?," "What is bioluminescence?," and "How does plankton shape affect plankton movement?" Each activity contains hyperlinks to pertinent materials, maps, and information to complete the activity.

Making Waves

169

The Amazing Red Planet  

NSDL National Science Digital Library

The purpose of this lesson is to introduce students to the planet Mars. This lesson will begin by discussing the location and size of Mars relative to Earth, as well as introduce many interesting facts about this red planet. Next, the history of Martian exploration is reviewed and students discover why scientists are so interested in studying this mysterious planet. The lesson concludes with students learning about future plans to visit Mars.

Integrated Teaching And Learning Program

170

Red and Black Experiment  

NSDL National Science Digital Library

This resource consists of a Java applet and expository text. The applet simulates the red and black experiment in which a player bets on a sequence of Bernoulli trials until a target fortune is reached or the player is ruined. The initial fortune, target fortune, and trial win probability can be varied, and the user can select either of two basic strategies: timid play or bold play.

Siegrist, Kyle

171

Great Red Spot (GRS)  

NASA Astrophysics Data System (ADS)

A huge permanent anticyclone in Jupiter's southern hemisphere, visible as a reddish oval at just over 20 °S. The earliest unequivocal observation was by Heinrich Schwabe in 1831 (the often-quoted sighting by Robert Hooke in 1664 now seems to have been of a similar but different spot). The GRS became a striking feature around 1880, when it developed a deep red coloration. It was also prominent in ...

Murdin, P.

2000-11-01

172

Evaluation of radiation effects on hematopoetic bone marrow by immunoscintigraphy  

SciTech Connect

Radiotherapy is known to cause dose-dependent damage to the hematopoetic bone marrow (HBM) within the portal. It was the aim of the present study to evaluate acute suppression and long term recovery of HBM by use of bone marrow immunoscintigraphy (BMI) with monoclonal antibodies (1 mg of intact BW 250/183 labelled with 350-400 MBq Tc-99m) against NCA-95, expressed on granulocytes and their precursor cells. Ninety-five planar scintigrams covering 114 portals were analyzed. Antibody uptake of irradiated bone marrow was quantified by ROI-technique and expressed as percentage of uptake in corresponding areas outside the portal. During irradiation a marked drop of marrow uptake significantly correlating with the already received dose was observed. Scans obtained after completion of radiotherapy revealed a reduced uptake ({approximately}40% of the reference region) for about 4 years. Afterwards bone marrow normalized in portals with doses <35 Gy while following >35 Gy diminished uptake (70{plus_minus}25%) persisted indicating irreversible damage to HBM. We conclude that BMI is suitable for evaluation of acute damage and long time recovery of functional bone marrow after therapeutic irradiation and may be used for optimized planning of repeated radiotherapy.

Dohmen, B.M.; Bares, R.; Buell, U. [Technical Univ. of Aachen (Germany)] [and others

1994-05-01

173

Dosimetry considerations of bone-seeking radionuclides for marrow ablation.  

PubMed

Marrow ablation by radionuclide therapy for patients requiring bone marrow transplantation is possible by injecting bone-seeking radiopharmaceuticals. For each radionuclide under consideration, one should determine the (1) amount of activity required to deliver a given radiation dose to marrow, (2) waiting period before reinfusion to limit the radiation dose to the transplant marrow to an acceptable level, and (3) dose to other critical organs. In an attempt to answer these questions, dose estimates for several radionuclides of interest (32P, 90Y, 153Sm, 166Ho, 186Re, and 188Re) have been calculated. All doses are derived following the MIRD methodology. Biodistribution data of 153Sm-labeled phosphates in the rat are used to estimate uptake of similar radiopharmaceuticals in humans. Typically the skeleton retains 50% of the injected activity and 50% is excreted rapidly through the urine, permitting delivery of ablation doses to the marrow, with tolerable doses to other organs. Skeletal retention data measured from a diagnostic dose can be used to calculate the activity required to deliver a desired marrow ablation dose consistent with toxicity limits set by other critical organs. PMID:8413017

Bayouth, J E; Macey, D J

1993-01-01

174

Red Tide in the Northeast  

NSDL National Science Digital Library

This Rhode Island Sea Grant fact sheet provides information about red tide algal blooms in New England. It addresses where and when red tides occur, how to know if shellfish are safe to consume, and other important facts about red tide. The site features a black and white picture of the dinoflagellate responsible for red tide in New England and a regional map of where infected shell fish have been found. It also features links to other red tide related sites and a list of pertinent reading material.

Elenor Ely

175

Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques  

PubMed Central

Background Prolonged, altered hematopoietic reconstitution is commonly observed in patients undergoing myeloablative conditioning and bone marrow and/or mobilized peripheral blood-derived stem cell transplantation. We studied the reconstitution of myeloid and lymphoid compartments after the transplantation of autologous CD34+ bone marrow cells following gamma irradiation in cynomolgus macaques. Results The bone marrow cells were first transduced ex vivo with a lentiviral vector encoding eGFP, with a mean efficiency of 72% ± 4%. The vector used was derived from the simian immunodeficiency lentivirus SIVmac251, VSV-g pseudotyped and encoded eGFP under the control of the phosphoglycerate kinase promoter. After myeloid differentiation, GFP was detected in colony-forming cells (37% ± 10%). A previous study showed that transduction rates did not differ significantly between colony-forming cells and immature cells capable of initiating long-term cultures, indicating that progenitor cells and highly immature hematopoietic cells were transduced with similar efficiency. Blood cells producingeGFP were detected as early as three days after transplantation, and eGFP-producing granulocyte and mononuclear cells persisted for more than one year in the periphery. Conclusion The transplantation of CD34+ bone marrow cells had beneficial effects for the ex vivo proliferation and differentiation of hematopoietic progenitors, favoring reconstitution of the T- and B-lymphocyte, thrombocyte and red blood cell compartments. PMID:18565229

Derdouch, Sonia; Gay, Wilfried; Nègre, Didier; Prost, Stéphane; Le Dantec, Mikael; Delache, Benoît; Auregan, Gwenaelle; Andrieu, Thibault; Leplat, Jean-Jacques; Cosset, François-Loïc; Le Grand, Roger

2008-01-01

176

Melatonin as a principal component of red light therapy.  

PubMed

Melatonin is well recognized for its role as a potent antioxidant and is directly implicated in the free radical theory of aging [1] [Reiter RJ, Pablos MI, Agapito TT, Guerrero JM. Melatonin in the context of the free radical theory of aging. Ann N Y Acad Sci 1996;786:362-78]. Moreover, melatonin has been shown to retard age-related increases in lipid peroxidation and oxidative damage [2] [Okatani Y, Wakatsuki A, Reiter RJ. Melatonin protects hepatic mitochondrial respiratory chain activity in senescence-accelerated mice. J Pineal Res 2002;32:143-8] and to act directly upon the immune system [3] [Poon AM, Liu ZM, Pang CS, Brown GM, Pang SF. Evidence for a direct action of melatonin on the immune system. Biol Signals 1994;3:107-17]. This report focuses on characterizing documented functions of melatonin in the context of red light therapy and proposes that melatonin is a potential mediator of red light's therapeutic effects, a hypothesis that is as yet untested. Red light therapy (670 nm, 4J/cm(2)) has been shown to restore glutathione redox balance upon toxicological insult and enhance both cytochrome c oxidase and energy production, all of which may be affected by melatonin. The red light treatment has also been successfully implemented in the clinical setting for its effectiveness in reducing both the number of incidences and severity of oral mucositis resulting in part from the chemotherapy and/or radiation administered prior to bone marrow transplants. Moreover, red light therapy improves wound healing and is being further tested for its ability to ameliorate toxicant-induced retinal and visual cortical neuron damage. Researchers in the growing field of light therapy may be in a position to draw from and collaborate with melatonin researchers to better characterize this alternative treatment. PMID:17321060

Yeager, Ronnie L; Oleske, Deanna A; Sanders, Ruth A; Watkins, John B; Eells, Janis T; Henshel, Diane S

2007-01-01

177

Transplantation immunology: Solid Organ and bone marrow  

PubMed Central

Development of the field of organ and tissue transplantation has accelerated remarkably since the human major histocompatibility complex (MHC) was discovered in 1967. Matching of donor and recipient for MHC antigens has been shown to have a significant positive effect on graft acceptance. The roles of the different components of the immune system involved in the tolerance or rejection of grafts and in graft-versus-host disease have been clarified. These components include: antibodies, antigen presenting cells, helper and cytotoxic T cell subsets, immune cell surface molecules, signaling mechanisms and cytokines that they release. The development of pharmacologic and biological agents that interfere with the alloimmune response and graft rejection has had a crucial role in the success of organ transplantation. Combinations of these agents work synergistically, leading to lower doses of immunosuppressive drugs and reduced toxicity. Reports of significant numbers of successful solid organ transplants include those of the kidneys, liver, heart and lung. The use of bone marrow transplantation for hematological diseases, particularly hematological malignancies and primary immunodeficiencies, has become the treatment of choice in many of these conditions. Other sources of hematopoietic stem cells are also being used, and diverse immunosuppressive drug regimens of reduced intensity are being proposed to circumvent the mortality associated with the toxicity of these drugs. Gene therapy to correct inherited diseases by infusion of gene-modified autologous hematopoietic stem cells has shown efficacy in two forms of severe combined immunodeficiency, providing an alternative to allogeneic tissue transplantation. PMID:20176267

Chinen, Javier; Buckley, Rebecca H.

2010-01-01

178

Radiation dose distribution within the bone marrow of patients receiving holmium-166-labeled-phosphonate for marrow ablation.  

PubMed

The primary objective of this work was to estimate the absorbed dose distribution to the bone marrow of six multiple myeloma patients who received holmium-166 (166Ho) DOTMP (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetramethylene-phosphonic acid) for the purpose of bone marrow ablation. A methodology based on gamma camera images was developed to estimate the regional absorbed dose distributions delivered to the bone marrow, and this was compared with values calculated from the MIRD technique and bone marrow biopsies. The activity concentration in various skeletal regions was calculated from the activity in the region of interest (ROI) drawn on whole body gamma camera images, and the mass of bone in each ROI was derived from a dual x-ray absorptiometry image. The radiation absorbed dose to the bone marrow was calculated from this activity concentration using an adaptation of Bragg-Gray cavity theory. The radiation absorbed dose delivered to the bone marrow in the six patients calculated from the MIRD "S" factors ranged from 15.0 to 46.3 Gy. The gamma camera measured activity concentration in skeletal regions predominantly composed of trabecular bone was approximately five to six times higher than that in cortical regions. The skeletal activity concentration in each patient ranged from highs in such regions as the ribs to lows in such regions as the shin and foot by a factor of nearly 20, producing a heterogeneous distribution of absorbed dose within the marrow. Dose volume histograms calculated for these patients indicated that 15%-20% of the marrow received an absorbed dose significantly larger than the average value, while 5%-10% of the marrow received a substantially lower dose. Weighted mean dose estimates from the regional technique were typically 30% greater than the average dose estimates calculated with the MIRD "S" factors. Finally, absorbed dose estimates for the marrow calculated from the regional technique correlated more closely with the clinical response of blood cells and abnormal proteins measured in bone marrow aspirates and peripheral blood samples than estimates from the MIRD "S" factors. PMID:7565363

Bayouth, J E; Macey, D J; Boyer, A L; Champlin, R E

1995-06-01

179

Understanding donors' motivations: a study of unrelated bone marrow donors.  

PubMed

Medical advances in bone marrow transplantation techniques and immunosuppressive medications have dramatically increased the number of such transplants performed each year, and consequently, the demand for bone marrow from unrelated donors. Although physiological aspects of bone marrow donation have been thoroughly investigated, very few studies have examined psychosocial factors that may impact individuals' donation decisions and outcomes. To examine one particular set of donor psychosocial issues, this study investigated motives for bone marrow donation among 343 unrelated bone marrow donors who donated through the National Marrow Donor Program. Six distinct types of donor motives were identified from open-ended questionnaire responses. Donors most frequently reported motives reflecting some awareness of both the costs (to themselves) and potential benefits (to themselves and the recipient) of donation. A desire to act in accordance with social or religious precepts, expected positive feelings about donating, empathy for the recipient, and the simple desire to help another person were also commonly cited reasons for donating. Among a series of donor background characteristics, donors' gender was the variable most strongly associated with motive type; women were most likely to cite expected positive feelings, empathy, and the desire to help someone. Central study findings indicated that donor motives predicted donors reactions to donation even after the effects of donor background characteristics (including gender) were controlled. Donors who reported exchange motives (weighing costs and benefits) and donors who reported simple (or idealized) helping motives experienced the donation as less positive in terms of higher predonation ambivalence and negative postdonation psychological reactions than did remaining donors. Donors who reported positive feeling and empathy motives had the most positive donation reactions in terms of lower ambivalence, and feeling like better persons postdonation. These finding add substantially to the body of work concerning medical volunteerism generally, and also have important practical implications for the recruitment and education of potential bone marrow donors. PMID:9203278

Switzer, G E; Dew, M A; Butterworth, V A; Simmons, R G; Schimmel, M

1997-07-01

180

Red Spot Movie  

NASA Technical Reports Server (NTRS)

This brief movie shows counterclockwise atmospheric motion around Jupiter's Great Red Spot. The clip was made from blue-filter images taken with the narrow-angle camera on NASA's Cassini spacecraft during seven separate rotations of Jupiter between Oct. 1 and Oct. 5, 2000.

The clip also shows the eastward and westward motion of the zonal jets, seen as the horizontal stripes flowing in opposite directions. The zonal jets circle the planet. As far as can be determined from both Earth-based and spacecraft measurements, the positions and speeds of the jets have not changed for 100 years. Since Jupiter is a fluid planet without a solid boundary, the jet speeds are measured relative to Jupiter's magnetic field, which rotates, wobbling like a top because of its tilt, every 9 hours 55.5 minutes. The movie shows motions in the magnetic reference frame, so winds to the west correspond to features that are rotating a little slower than the magnetic field, and eastward winds correspond to features rotating a little faster.

Because the Red Spot is in the southern hemisphere, the direction of motion indicates it is a high-pressure center. Small bright clouds appear suddenly to the west of the Great Red Spot. Scientists suspect these small white features are lightning storms. The storms eventually merge with the Red Spot and surrounding jets, and may be the main energy source for the large-scale features.

The smallest features in the movie are about 500 kilometers (about 300 miles) across. The spacing of the movie frames in time is not uniform; some consecutive images are separated by two Jupiter rotations, and some by one. The images have been re-projected using a simple cylindrical map projection. They show an area from 50 degrees north of Jupiter's equator to 50 degrees south, extending 100 degrees east-west, about one quarter of Jupiter's circumference.

Cassini is a cooperative project of NASA, the European Space Agency and the Italian Space Agency. The Jet Propulsion Laboratory, a division of the California Institute of Technology in Pasadena, manages the Cassini mission for NASA's Office of Space Science, Washington, D.C.

2000-01-01

181

PLANT BIOLOGY: Seeing Red  

NSDL National Science Digital Library

Access to the article is free, however registration and sign-in are required. Although flavonoids contribute to the red, violet, and blue pigmentation of many flowers and seeds, their biosynthetic pathway is still being elucidated. In their Perspective, Bartel and Matsuda discuss new work (Xie et al.) revealing that the biosynthetic enzyme BANYULS is important for regulating the relative amounts of two types of flavonoids, the anthocyanins and the condensed tannins, both of which are powerful antioxidants that confer health benefits on humans when eaten.

Bonnie Bartel (Rice University; Department of Biochemistry and Cell Biology)

2003-01-17

182

Red microchip VECSEL array  

Microsoft Academic Search

We report an InGaP\\/AlInGaP\\/GaAs microchip vertical-external-cavity surface emitting laser operating directly at red wavelengths and demonstrate its potential for array-format operation. Optical pumping with up to 3.3W at 532nm produced a maximum output power of 330mW at 675nm, in a single circularly-symmetric beam with M2<2. Simultaneous pumping with three separate input beams, generated using a diffractive optical element, achieved lasing

Jennifer E. Hastie; Lynne G. Morton; Stephane Calvez; Martin D. Dawson; Tomi Leinonen; Markus Pessa; Graham Gibson; Miles J. Padgett

2005-01-01

183

Acquired pure red cell aplasia: updated review of treatment  

PubMed Central

Pure red cell aplasia (PRCA) is a syndrome characterized by a severe normocytic anaemia, reticulocytopenia, and absence of erythroblasts from an otherwise normal bone marrow. Primary PRCA, or secondary PRCA which has not responded to treatment of the underlying disease, is treated as an immunologically-mediated disease. Although vigorous immunosuppressive treatments induce and maintain remissions in a majority of patients, they carry an increased risk of serious complications. Corticosteroids were used in the treatment of PRCA and this has been considered the treatment of first choice although relapse is not uncommon. Cyclosporine A (CsA) has become established as one of the leading drugs for treatment of PRCA. However, common concerns have been the number of patients treated with CsA who achieve sustained remissions and the number that relapse. This article reviews the current status of CsA therapy and compares it to other treatments for diverse PRCAs. PMID:18510682

Sawada, Kenichi; Fujishima, Naohito; Hirokawa, Makoto

2008-01-01

184

Molecular and histopathological detection of Hepatozoon canis in red foxes (Vulpes vulpes) from Portugal  

PubMed Central

Background Hepatozoon canis is a protozoan tick-borne pathogen of dogs and wild canids. Hepatozoon spp. have been reported to infect foxes in different continents and recent studies have mostly used the polymerase chain reaction (PCR) for the detection and characterization of the infecting species. Surveying red foxes (Vulpes vulpes) may contribute to better understanding the epidemiology of canine vector-borne diseases, including hepatozoonosis caused by H. canis in domestic dogs. The present study investigated the prevalence of Hepatozoon spp. by means of histopathology and molecular analysis of different tissues in red foxes from different parts of Portugal. Methods Blood and tissues including bone marrow, heart, hind leg muscle, jejunum, kidney, liver, lung, popliteal or axillary lymph nodes, spleen and/or tongue were collected from 91 red foxes from eight districts in northern, central and southern Portugal. Tissues were formalin-fixed, paraffin-embedded, cut and stained with hematoxylin and eosin. Polymerase chain reaction (PCR) amplified a ~650 bp fragment of the 18S rRNA gene of Hepatozoon spp. and the DNA products were sequenced. Results Hepatozoon canis was detected in 68 out of 90 foxes (75.6%) from all the sampled areas by PCR and sequencing. Histopathology revealed H. canis meronts similar in shape to those found in dogs in the bone marrow of 11 (23.4%) and in the spleen of two (4.3%) out of 47 foxes (p?=?0.007). All the 11 foxes found positive by histopathology were also positive by PCR of bone marrow and/or blood. Positivity by PCR (83.0%) was significantly higher (p?marrow samples from the same 47 foxes. Sequences of the 18S rRNA gene of H. canis were 98–99% identical to those in GenBank. Conclusions Hepatozoon canis was found to be highly prevalent in red fox populations from northern, central and southern Portugal. Detection of the parasite by histopathology was significantly less sensitive than by PCR. Red foxes are a presumptive reservoir of H. canis infection for domestic dogs. PMID:24655375

2014-01-01

185

Red - Take a Closer Look  

PubMed Central

Color research has shown that red is associated with avoidance of threat (e.g., failure) or approach of reward (e.g., mating) depending on the context in which it is perceived. In the present study we explored one central cognitive process that might be involved in the context dependency of red associations. According to our theory, red is supposed to highlight the relevance (importance) of a goal-related stimulus and correspondingly intensifies the perceivers’ attentional reaction to it. Angry and happy human compared to non-human facial expressions were used as goal-relevant stimuli. The data indicate that the color red leads to enhanced attentional engagement to angry and happy human facial expressions (compared to neutral ones) - the use of non-human facial expressions does not bias attention. The results are discussed with regard to the idea that red induced attentional biases might explain the red-context effects on motivation. PMID:25254380

Buechner, Vanessa L.; Maier, Markus A.; Lichtenfeld, Stephanie; Schwarz, Sascha

2014-01-01

186

The red ear syndrome  

PubMed Central

Red Ear Syndrome (RES) is a very rare disorder, with approximately 100 published cases in the medical literature. Red ear (RE) episodes are characterised by unilateral or bilateral attacks of paroxysmal burning sensations and reddening of the external ear. The duration of these episodes ranges from a few seconds to several hours. The attacks occur with a frequency ranging from several a day to a few per year. Episodes can occur spontaneously or be triggered, most frequently by rubbing or touching the ear, heat or cold, chewing, brushing of the hair, neck movements or exertion. Early-onset idiopathic RES seems to be associated with migraine, whereas late-onset idiopathic forms have been reported in association with trigeminal autonomic cephalalgias (TACs). Secondary forms of RES occur with upper cervical spine disorders or temporo-mandibular joint dysfunction. RES is regarded refractory to medical treatments, although some migraine preventative treatments have shown moderate benefit mainly in patients with migraine-related attacks. The pathophysiology of RES is still unclear but several hypotheses involving peripheral or central nervous system mechanisms have been proposed. PMID:24093332

2013-01-01

187

Red face revisited: Flushing.  

PubMed

The term red face is reserved for lesions located exclusively or very predominantly on the face that result from changes in cutaneous blood flow triggered by multiple different conditions. Facial erythema may not only present clinically as a distinct entity, but can also be a sign of other diseases. Patients with a red face challenge clinicians to consider a broad differential diagnosis. Diagnosis is based on date and mode of appearance, characteristics of the erythema, functional signs, and associated systemic manifestations. In most cases, the cause is a benign disease such as rosacea, contact dermatitis, photodermatosis, and climacterium, and a thorough history and physical examination is enough to make a diagnosis; facial erythema may also present as a symptom of drug allergies, cardiac disease, carcinoid syndrome, pheochromocytoma, mastocytosis, and anaphylaxis, as well as some rare causes such as medullary carcinoma of the thyroid, pancreatic cell tumor, and renal carcinoma where further laboratory, radiologic, or histopathologic studies are required. In this review, the mechanisms of flushing, its clinical differential diagnosis, and management of various conditions that cause flushing are discussed. PMID:25441473

?kizo?lu, Güliz

2014-01-01

188

The challenging world of cytopenias: distinguishing myelodysplastic syndromes from other disorders of marrow failure.  

PubMed

Over the past decade, our understanding of bone marrow failure has advanced considerably. Marrow failure encompasses multiple overlapping diseases, and there is increasing availability of diagnostic tools to distinguish among the subtypes. Identification of genetic alterations that underlie marrow failure has also greatly expanded, especially for myelodysplastic syndromes. Molecular markers are increasingly used to guide the management of myelodysplasia and may distinguish this diagnosis from other marrow failure disorders. This review summarizes the current state of distinguishing among causes of marrow failure and discusses the potential uses of multiple diagnostic and prognostic indicators in the management of myelodysplastic syndromes and other bone marrow failure disorders. PMID:24899643

DeZern, Amy E; Sekeres, Mikkael A

2014-07-01

189

www.vopen.net 1994 Young Mark Ewing Red Hat Red H  

E-print Network

www.vopen.net Linux --- 1994 Young Mark Ewing Red Hat Red H at Linux Linux Red Hat Linux Red Hat Linux Red Hat Linux MandrakeSOT BestConnectivaAbit KondaraRed Hat Linux 2001 4 16 Red Hat Red Hat Linux 7.1 Red Hat Linux 1 1.1 1 Linux 2.4.2 2 Xfree86 4.0.3 3 4 Big5 5 CPU CPU 4

Li, Tiejun

190

Use of long-term human marrow cultures to demonstrate progenitor cell precursors in marrow treated with 4-hydroperoxycyclophosphamide  

SciTech Connect

The continued retrieval of progenitor cells (CFU-GEMM, BFU-E, CFU-E, CFU-GM) from human long-term marrow cultures (LTMC) is not uncommonly used as evidence that proliferation and differentiation are occurring in more primitive hematopoietic stem cells (HSC) in these cultures. Alternatively, the continued presence of progenitors in LTMC could be the result of survival and/or limited self-renewal of progenitor cells present when the culture was initiated, and such progenitors would have little relevance to the parent HSC. The following studies were designed to determine the relative contributions of precursors of progenitor cells to the total progenitor cells present in LTMC using a two-stage regeneration model. The adherent layer in LTMC was established over 3 weeks, irradiated (875 rad) to permanently eliminate resident hematopoietic cells, and recharged with autologous cryo-preserved marrow that was either treated or not treated (control) with 4-hydroperoxycyclophosphamide (4-HC, 100 micrograms/ml for 30 min). The 4-HC-treated marrow contained no progenitor cells, yet based on clinical autologous bone marrow transplant experience, has intact HSC. Within 1-3 weeks, progenitor cells reappeared in the irradiated LTMC recharged with 4-HC-treated marrow, and were preferentially located in the adherent layer. By 2-6 weeks, the number of progenitor cells in the adherent layer of LTMC recharged with 4-HC marrow was equivalent to control LTMC. The progenitors regenerating in the irradiated LTMC recharged with 4-HC-treated marrow appear to originate from precursors of progenitor cells, perhaps HSC. We propose this model may be useful in elucidating cellular and molecular correlates of progenitor cell regeneration from precursors.

Winton, E.F.; Colenda, K.W.

1987-07-01

191

International red meat trade.  

PubMed

The maturation of the US beef and pork markets and increasing consumer demands for convenience, safety, and nutrition suggests that the beef and pork industries must focus on product development and promotion. New marketing arrangements are developing that help coordinate production with consumer demands. The relative high levels of incomes in the United States are likely to increase the demands for branded products rather than increase total per capita consumption. Foreign markets represent the greatest opportunity for increased demand for commodity beef and pork products. Increasing incomes in developing countries will likely allow consumers to increase consumption of animal-source proteins. Real prices of beef and pork have declined substantially because of sagging domestic demand and increasing farm-level production technologies. Increasing US beef and pork exports have obviated some of the price declines. Pork attained a net export position from a quantity perspective in 1995. The United States continues to be a net importer of beef on a quantity basis but is close to becoming a net exporter in terms of value. By-products continue to play a critical role in determining the red meat trade balance and producer prices. The United States, however, must continue to become cost, price, and quality competitive with other suppliers and must secure additional market access if it is to sustain recent trade trends. Several trade tensions remain in the red meat industry. For example, mandated COOL will undoubtedly have domestic and international effects on the beef and pork sectors. Domestically, uncertainty regarding consumer demand responses or quality perceptions regarding product origin, as well as added processor-retailer costs will be nontrivial. How these factors balance out in terms of benefits versus costs to the industry is uncertain. From an international perspective, some beef and pork export suppliers to the United States could view required labeling as a trade restriction, which could ultimately impact future US red meat exports. Conversely, some countries may view such labeling requirements as an opportunity to brand high-quality products. The US lamb meat industry has experienced declining real prices, domestic production, and demand. The cessation of wool incentive payments, increased environmental regulations, and competition by imports have significantly affected the industry. Import suppliers have capitalized on product quality in this niche market. Trade restrictions initially imposed in 1999 by the US Government were ruled illegal by the WTO. The US Government responded by providing financial assistance to lamb producers. Product quality improvements and promotion aimed at the domestic market, however, will be critical factors in shaping the economic viability of the US lamb meat industry. PMID:12951744

Brester, Gary W; Marsh, John M; Plain, Ronald L

2003-07-01

192

Risk factors in interstitial pneumonitis following allogenic bone marrow transplantation  

SciTech Connect

Total body irradiation is part of the preparatory regimen for allogeneic bone marrow transplantation because of its cytotoxic and immunosuppressive properties. A major toxicity of bone marrow transplantation has been interstitial pneumonitis, which may be, in part, related to the lung irradiation. One hundred and sixty-one consecutive patients receiving allogeneic bone marrow transplantation for leukemia and aplastic anemia at Johns Hopkins Hospital (1968-1979) were retrospectively studied. The present study demonstrated that lung shielding to 600 rad maximum in single dose total body irradiation, fractionation of total body irradiation in comparison to single dose total body irradiation, and absence of graft versus host disease in the leukemia patients, each reduced the risk of interstitial pneumonitis. Total body irradiation significantly reduced the leukemia recurrence rate and/or the failure of remission induction.

Pino Y Torres, J.L.; Bross, D.S.; Lam, W.C.; Wharam, M.D.; Santos, G.W.; Order, S.E.

1982-08-01

193

Receptor conversion in carcinoma breast metastatic to the bone marrow.  

PubMed

Breast cancer is the first in the incidence of malignancies among women. The documented frequency of osseous metastasis from breast cancer is about 12.8 %. Although breast cancer cells have been documented in the bone marrow in autopsy, haematological manifestations due to metastatic disease to the marrow is rare. The prognosis of these cases is dismal and improper management worsen the condition further. IHC for ER, PR & Her 2/neu status plays a vital role in ascertaining the treatment protocol both in primary tumors as well as metastatic or recurrent tumors. Receptor conversion has been documented in metastatic and recurrent cases. We report a case of 65 year female with a history of ER, PR positive and Her 2/neu negative carcinoma breast 3½ years back, presented with anemia and low back ache diagnosed as metastatic breast carcinoma to bone marrow with receptor conversion. PMID:25332613

Santosh, T; Patro, M K; Nayak, J; Bal, A K; Choudhury, A; Behera, B; Mishra, D P

2014-09-01

194

Bone marrow necrosis in a girl with Hodgkin's disease.  

PubMed

Bone marrow necrosis (BMN) is a rare finding in children with malignancy occurring most commonly in children with acute lymphoblastic leukemia. This article describes the first case of a girl who developed BMN during treatment for Hodgkin's disease. During the second cycle of chemotherapy, she experienced sudden profound bone pain in the lumbosacral region associated with elevated levels of lactate dehydrogenase (LDH), fibrin degradation products (D-Dimer), and alkaline phosphatase as well as pancytopenia and leukoerythroblastosis. MRI studies showed multiple confluent areas with low signal intensity and rim contrast enhancement in all vertebral bodies. Bone marrow biopsy revealed focal necrosis within hypocellular bone marrow. The patient responded quickly to symptomatic treatment with analgetics and heparin; however, elevations of LDH and D-Dimer persisted for 1.5 and 8 months, respectively. Clinicians should be aware of this rare condition to establish the diagnosis and to continue oncologic treatment as early as possible. PMID:22773298

Lackner, Herwig; Strenger, Volker; Sovinz, Petra; Beham-Schmid, Christine; Pilhatsch, Alexander; Benesch, Martin; Schwinger, Wolfgang; Ulreich, Raphael; Schmidt, Sandrin; Urban, Christian

2012-09-01

195

Robust conversion of marrow cells to skeletal muscle with formation of marrow-derived muscle cell colonies: A multifactorial process  

SciTech Connect

Murine marrow cells are capable of repopulating skeletal muscle fibers. A point of concern has been the robustness of such conversions. We have investigated the impact of type of cell delivery, muscle injury, nature of delivered cell, and stem cell mobilizations on marrow to muscle conversion. We transplanted GFP transgenic marrow into irradiated C57BL/6 mice and then injured anterior tibialis muscle by cardiotoxin. One month after injury, sections were analyzed by standard and deconvolutional microscopy for expression of muscle and hematopietic markers. Irradiation was essential to conversion although whether by injury or induction of chimerism is not clear. Cardiotoxin and to a lesser extent PBS injected muscles showed significant number of GFP+ muscle fibers while uninjected muscles showed only rare GFP+ cells. Marrow conversion to muscle was increased by two cycles of G-CSF mobilization and to a lesser extent with G-CSF and steel or GM-CSF. Transplantation of female GFP to male C57 BL/6 and GFP to Rosa26 mice showed fusion of donor cells to recipient muscle. High numbers of donor derived muscle colonies and up to12 percent GFP positive muscle cells were seen after mobilization or direct injection. These levels of donor muscle chimerism approach levels which could be clinically significant in developing strategies for the treatment of muscular dystrophies. In summary, the conversion of marrow to skeletal muscle cells is based on cell fusion and is critically dependent on injury. This conversion is also numerically significant and increases with mobilization.

Abedi, Mehrdad; Greer, Deborah A.; Colvin, Gerald A.; Demers, Delia A.; Dooner, Mark S.; Harpel, Jasha A.; Weier, Heinz-Ulrich G.; Lambert, Jean-Francois; Quesenberry, Peter J.

2004-01-10

196

Angry Red Planet  

NSDL National Science Digital Library

This mystery puts the reader in control, Mission Control, as he/she helps with a simulated flight to Mars. In this simulation, four "bionauts" are sealed into identical pods containing plants, animals and water. The goal is for them to survive for six months receiving no water, food or air from outside. The reader monitors the conditions in each pod, simulating Misson Control back on Earth. This story begins on the 34th day of the simulation, when the reader notices something wrong in one of the pods. The oxygen is getting low - why? Besides the usual Science Mystery themes (literacy, inquiry-based learning, problem-solving logic, inductive and deductive reasoning), "Angry Red Planet" puts your students hands-on with facts about respiration, ecosystems and ecological cycles, chemical and biochemical reactions, carbon dioxide poisoning, and the effects of stress on human physiology and psychology. They must learn how to read graphs and evaluate data to solve the mystery.

BEGIN:VCARD VERSION:2.1 FN:Ken Eklund N:Eklund;Ken ORG:WriterGuy REV:2005-04-06 END:VCARD

2000-12-15

197

Red microchip VECSEL array.  

PubMed

We report an InGaP/AlInGaP/GaAs microchip vertical-external-cavity surface emitting laser operating directly at red wavelengths and demonstrate its potential for array-format operation. Optical pumping with up to 3.3W at 532nm produced a maximum output power of 330mW at 675nm, in a single circularly-symmetric beam with M2<2. Simultaneous pumping with three separate input beams, generated using a diffractive optical element, achieved lasing from three discrete areas of the same chip. Output power of ~95mW per beam was obtained from this 3x1 array, each beam having a Gaussian intensity profile with M2<1.2. In a further development, a spatial light modulator allowed computer control over the orientation and separation of the pump beams, and hence dynamic control over the configuration of the VECSEL array. PMID:19498743

Hastie, Jennifer; Morton, Lynne; Calvez, Stephane; Dawson, Martin; Leinonen, Tomi; Pessa, Markus; Gibson, Graham; Padgett, Miles

2005-09-01

198

Ambiguous red shifts  

NASA Astrophysics Data System (ADS)

A one-parameter conformal invariance of Maxwell's equations allows the wavelengths of electromagnetic waves to change as they propagate, and do so even in otherwise field-free space. This produces an ambiguity in interpretations of stellar red shifts. Experiments that will determine the value of the group parameter, and thereby remove the ambiguity, are proposed. They are based on an analysis of the anomalous frequency shifts uncovered in the Pioneer 10 and 11 spacecraft studies, and physical interpretation of an isomorphism discovered by E.L. Hill. If the group parameter is found to be non-zero, Hubble's relations will have to be reinterpreted and space-time metrics will have to be altered. The cosmological consequences of the transformations are even more extensive because, though they change frequencies they do not alter the energy and momentum conservation laws of classical and quantum-electrodynamical fields established by Cunningham and by Bia?ynicki-Birula.

Wulfman, Carl E.

2010-12-01

199

Classification of red variables  

NASA Technical Reports Server (NTRS)

Red variables are traditionally classified into Mira, semiregular (SR), and slow irregular (L) variables. The Mira variables are the best defined subgroup, whereas SR and L stars are more numerous. The SR subgroup is additionally subdivided into: SRa variables, which feature regular variability with smaller pulsation amplitudes than Miras; SRb variables, which are less regular; SRc variables, which are more luminous; and SRd variables, which are warmer. Relationships within each group are not clear. An analysis of long-term American Association of Variable Star Observers (AAVSP) light curves is reported on. It is found that Mira-type variables are clearly different and distinguishable from SR variables. Similarly, M-type Miras and C-type Miras feature different light curve properties. The M-Miras form a homogeneous group. The pulsations of SR variables are unstable.

Mattei, Janet A.; Foster, Grant; Hurwitz, Leora A.; Malatesta, Kerriann H.; Willson, Lee Anne; Mennessier, Marie-Odile

1997-01-01

200

Bone marrow cells as precursors of the tumor stroma  

PubMed Central

Cancer is a systemic disease. Local and distant factors conspire to promote or inhibit tumorigenesis. The bone marrow is one important source of tumor promoting cells. These include the important mature and immature hematopoietic cells as well as circulating mesenchymal progenitors. Recruited bone marrow cells influence carcinogenesis at the primary site, within the lymphoreticular system and even presage metastasis through their recruitment to distant organs. In this review we focus on the origins and contribution of cancer-associated fibroblasts in tumorigenesis. Mesenchymal cells present an important opportunity for targeted cancer prevention and therapy. PMID:23499739

Worthley, Daniel L.; Si, Yiling; Quante, Michael; Churchill, Michael; Mukherjee, Siddhartha; Wang, Timothy C.

2014-01-01

201

Zinc toxicity: denture adhesives, bone marrow failure and polyneuropathy.  

PubMed

A 36-year-old female developed bone marrow failure diagnosed as myelodysplastic syndrome (MDS) (Sidebar), followed shortly by a peripheral neuropathy and a gait disturbance. While waiting for a bone marrow transplant, she reported to us that she had seen attorney-generated, televised advertisements concerning the role of denture adhesives relating to her malady. Labs were then obtained demonstrating she had dramatic and unsuspected hypocupremia and hyperzincemia. Administration of copper and cessation of denture adhesives resulted in recovery of her hematopoietic system and partial resolution of the neurological sequela. PMID:22375440

Crown, Loren A; May, Jeffery A

2012-02-01

202

[Bronchiolitis obliterans organizing pneumonia (BOOP) after allogeneic bone marrow transplantation].  

PubMed

We report a patient who underwent allogeneic bone marrow transplantation for ALL. The patient presented low grade fever, cough and dyspnea at day 3 after after bone marrow transplantation. Imaging studies showed bilateral patchy infiltrates on chest X-ray and chest CT. Though treated by antibiotics, chest auscultation recognized marked bilateral inspiratory crackles. Transbronchial lung biopsy performed on day 34 showed bronchiolitis obliterans and an organizing interstitial pneumonia. The patient was treated with methylprednisolone and follow up computed tomography findings improved. PMID:8960660

Nakayama, M; Takahashi, S; Inoue, T; Nagamura, F; Setoyama, M; Ooi, J; Takahashi, T; Oshima, Y; Miyamoto, K; Uemura, N; Shindo, E; Tajika, K; Yanagisawa, K; Nagayama, H; Okamoto, S; Tojo, A; Tani, K; Ozawa, K; Asano, S; Wakabayashi, T; Sato, N; Ichinose, Y; Toyama, K; Saito, H

1996-11-01

203

Marrow fat deposition and skeletal growth in caribou calves  

USGS Publications Warehouse

I evaluated rates of marrow fat deposition and skeletal growth of caribou (Rangifer tarandus granti) calves through 20 days of age at Denali National Park, Alaska, USA. Both were negatively correlated with late winter snowfall, indicating the prolonged effects of maternal undernutrition following severe winters. Using regression analyses, I found that the rates of marrow fat deposition and hindfoot growth during the 20 days following birth declined 46% and 68%, respectively, over the range of winter severity during this study. These measures of development may indicate a broader array of effects of maternal undernutrition, influencing the vulnerability of caribou calves to predation.

Adams, L.

2003-01-01

204

Bone marrow abnormalities in HIV infected children, report of three cases and review of the literature  

PubMed Central

Bone marrow abnormalities in HIV infected adults include hypocellularity, myelodysplasia and poor marrow recovery. Data in children is limited. We report a series of three HIV infected with varied bone marrow abnormalities. First child was a 7-year-old boy with pulmonary tuberculosis, anemia, thrombocytopenia and bone marrow examination showed hypoplastic marrow. He succumbed to his disease within seven days of hospitalization. Second child was a three and a half year old girl who had severe anemia and her bone marrow examination showed dyserythropoiesis. Third child was a 7-year-old boy who had splenic abscesses and pancytopenia and bone marrow examination showed myelofibrosis with increased plasma cells. He also succumbed due to a fatal pulmonary bleed. Thus, advanced HIV disease in children can lead to bone marrow suppression in form of hypoplasia or myelofibrosis which can be fatal. PMID:24778674

Shah, Ira; Murthy, Anuradha

2014-01-01

205

Paris, December 20th 2006 To the Editor, Biology and Bone Marrow Transplantation  

E-print Network

Paris, December 20th 2006 To the Editor, Biology and Bone Marrow Transplantation Philippe Ratajczak-27Sep2007 Author manuscript, published in "Biology of Blood and Marrow Transplantation 2007 hemorrhage (IAH) following hematopoietic stem cell transplantation (HSCT) highlights significant points

Paris-Sud XI, Université de

206

What to Expect during a Blood and Marrow Stem Cell Transplant  

MedlinePLUS

... Expect During a Blood and Marrow Stem Cell Transplant A blood and marrow stem cell transplant has ... whether you need chemotherapy or chemotherapy and radiation. Transplant A stem cell transplant is like a blood ...

207

Cellular response of the primate (M. mulatta) spleen to bone marrow transplantation in gamma irradiated recipients  

E-print Network

38. Hyperemia of gastrointestinal tract . 52 Gross pathology. Defatted marrow by cephalic vein 6F8. Icterus noted in tissues. No hemorrhage 53 12 Gross pathology. Defatted marrow by cephalic vein 6F8. Icteric tissues of the gastro- intestinal...

Fraunfelter, Frank Clare

2012-06-07

208

Effect of Synthetic Marrow on Synthetic Open-Cell Foam Vertebrae  

E-print Network

The intended purpose of this research is to further supplement the first generation of the Analogue Spine Model (ASM) by introducing a bone marrow component. Currently the ASM does not include a bone marrow component. While ...

Good, Brian Victor

2011-12-31

209

What to Expect After a Blood and Marrow Stem Cell Transplant  

MedlinePLUS

... What To Expect After a Blood and Marrow Stem Cell Transplant You’ll stay in the hospital for ... or even months after your blood and marrow stem cell transplant. Your doctors will want to be sure ...

210

The National Marrow Donor Program and Be The Match Registry | NIH MedlinePlus the Magazine  

MedlinePLUS

... the Blood and Marrow Transplant Clinical Trials Network (BMT CTN). These are funded by the National Heart, ... and Marrow Transplant Clinical Trials Network at the BMT CTN Web site, www.bmtctn.net . Patients helping ...

211

Red Means Go Information Technology  

E-print Network

, integrated security & legislative compliance. #12;Red Means Go Identity and Access Management ·PurposeRed Means Go Office of Information Technology Future Directions for OIT Spring Retreat Civil customer expectations. · Reliable Systems & Security Design, implement and manage all services and systems

212

Schubert edits JGR-Red  

Microsoft Academic Search

`As editor, my major concern will be to maintain and strengthen the Journal of Geophysical Research's position as the preeminent journal in solid earth and solid planet geophysics,' asserted Gerald Schubert, the new editor of the red section of JGR. `I will endeavor to ensure that all major subject areas covered by JGR-Red are adequately represented among the published papers.'

Barbara T. Richman

1982-01-01

213

Tuning RED for web traffic  

Microsoft Academic Search

We study the effects of RED on the performance of Web brows- ing with a novel aspect of our work being the use of a user- centric measure of performance — response time for HTTP request-response pairs. We empirically evaluate RED across a range of parameter settings and offered loads. Our results show that: (1) contrary to expectations, compared to

Mikkel Christiansen; Kevin Jeffay; David Ott; F. Donelson Smith

2000-01-01

214

Tide pushing shellfishers into red  

NSDL National Science Digital Library

This USA Today article provides very general information about the 2005 red tide outbreak off the Gulf of Maine. It offers possible explanations for the outbreak, what is being done to protect people from hazards related to red tide, and how the outbreak has devastated local shellfishers.

Hampson, Rick; Today, Usa

215

Advancement of pediatric blood and marrow transplantation research in North America: priorities of the Pediatric Blood and Marrow Transplant Consortium.  

PubMed

Advances in pediatric bone marrow transplantation (BMT) are slowed by the small number of patients with a given disease who undergo transplantation, a lack of sufficient infrastructure to run early-phase oncology protocols and studies of rare nonmalignant disorders, and challenges associated with funding multi-institutional trials. Leadership of the Pediatric Blood and Marrow Transplant Consortium (PBMTC), a large pediatric BMT clinical trials network representing 77 active and 45 affiliated centers worldwide, met in April 2009 to develop strategic plans to address these issues. Key barriers, including infrastructure development and funding, along with scientific initiatives in malignant and nonmalignant disorders, cellular therapeutics, graft-versus-host disease, and supportive care were discussed. The PBMTC's agenda for approaching these issues will result in infrastructure and trials specific to pediatrics that will run through the PBMTC or its partners, the Blood and Marrow Transplant Clinical Trials Network and the Children's Oncology Group. PMID:20079865

Pulsipher, Michael A; Horwitz, Edwin M; Haight, Ann E; Kadota, Richard; Chen, Allen R; Frangoul, Haydar; Cooper, Laurence J N; Jacobsohn, David A; Goyal, Rakesh K; Mitchell, David; Nieder, Michael L; Yanik, Gregory; Cowan, Morton J; Soni, Sandeep; Gardner, Sharon; Shenoy, Shalini; Taylor, Douglas; Cairo, Mitchell; Schultz, Kirk R

2010-09-01

216

Red yeast rice for dysipidemia.  

PubMed

Red yeast rice is an ancient Chinese food product that contains monacolins, chemical substances that are similar to statins in their mechanisms of action and lipid lowering properties. Several studies have found red yeast rice to be moderately effective at improving the lipid profile, particularly for lowering the low-density lipoprotein cholesterol levels. One large randomized controlled study from China found that red yeast rice significantly improved risk of major adverse cardiovascular events and overall survival in patients following myocardial infarction. Thus, red yeast rice is a potentially useful over-the-counter cholesterol-lowering agent. However, many red yeast rice formulations are non-standardized and unregulated food supplements, and there is a need for further research and regulation of production. PMID:24003656

Shamim, Shariq; Al Badarin, Firas J; DiNicolantonio, James J; Lavie, Carl J; O'Keefe, James H

2013-01-01

217

Rapid Increase in Marrow Fat Content and Decrease in Marrow Perfusion in Lumbar Vertebra Following Bilateral Oophorectomy: An MR Imaging-Based Prospective Longitudinal Study  

PubMed Central

Objective Bilateral oophorectomy leads to reduced bone mineral density (BMD), and reduced BMD is associated with increased marrow fat and reduced marrow perfusion. Purpose of this study was to investigate how soon these changes occur following surgical oophorectomy. Materials and Methods Six patients who underwent hysterectomy and bilateral salpingo-oophorectomy were studied. At baseline, mean patient age was 49.5 years (range: 45-54 years). Third lumbar vertebral body BMD measurement using quantitative CT, marrow fat fraction (FF) using MR spectroscopy and marrow perfusion using dynamic contrast enhanced MRI were conducted immediately prior to surgery and at 3, 9, and 21 months after surgery. Results Reduced BMD, increased marrow FF, and reduced marrow perfusion occurred synchronously post-oophorectomy. There was a sharp decrease of 12.5 ± 7.2% in BMD (n = 6), a sharp increase of 92.2 ± 46.3% (n = 6) in FF, a sharp decrease of 23.6 ± 3.9% in maximum contrast enhancement (n = 5), and of 45.4 ± 7.7% for enhancement slope (n = 5) during the initial 3 months post surgery. BMD and marrow perfusion continued to decrease, and marrow FF continued to increase at a slower rate during the following 18 months. Friedman test showed a significant trend for these changes (p < 0.05). Conclusion Bilateral oophorectomy leads to a rapid decrease in lumbar BMD, an increase in marrow fat content, and a decrease in marrow blood perfusion. PMID:25598684

Griffith, James F; Deng, Min; Yeung, David KW; Yuan, Jing

2015-01-01

218

Establishment of bone marrow and hematopoietic niches in vivo by reversion of chondrocyte differentiation of human bone marrow stromal cells.  

PubMed

Human bone marrow stromal cells (BMSCs, also known as bone marrow-derived "mesenchymal stem cells") can establish the hematopoietic microenvironment within heterotopic ossicles generated by transplantation at non-skeletal sites. Here we show that non-mineralized cartilage pellets formed by hBMSCs ex vivo generate complete ossicles upon heterotopic transplantation in the absence of exogenous scaffolds. These ossicles display a remarkable degree of architectural fidelity, showing that an exogenous conductive scaffold is not an absolute requirement for bone formation by transplanted BMSCs. Marrow cavities within the ossicles include erythroid, myeloid and granulopoietic lineages, clonogenic hematopoietic progenitors and phenotypic HSCs, indicating that complete stem cell niches and hematopoiesis are established. hBMSCs (CD146(+) adventitial reticular cells) are established in the heterotopic chimeric bone marrow through a unique process of endochondral bone marrow formation, distinct from physiological endochondral bone formation. In this process, chondrocytes remain viable and proliferate within the pellet, are released from cartilage, and convert into bone marrow stromal cells. Once explanted in secondary culture, these cells retain phenotype and properties of skeletal stem cells ("MSCs"), including the ability to form secondary cartilage pellets and secondary ossicles upon serial transplantation. Ex vivo, hBMSCs initially induced to form cartilage pellets can be reestablished in adherent culture and can modulate gene expression between cartilage and stromal cell phenotypes. These data show that so-called "cartilage differentiation" of BMSCs in vitro is a reversible phenomenon, which is actually reverted, in vivo, to the effect of generating stromal cells supporting the homing of hematopoietic stem cells and progenitors. PMID:24675053

Serafini, Marta; Sacchetti, Benedetto; Pievani, Alice; Redaelli, Daniela; Remoli, Cristina; Biondi, Andrea; Riminucci, Mara; Bianco, Paolo

2014-05-01

219

Disseminated toxoplasmosis in marrow recipients: a report of three cases and a review of the literature  

Microsoft Academic Search

Toxoplasma infection following bone marrow transplantation (BMT) is infrequently reported. We report three cases of disseminated toxoplasmosis in BMT recipients documented during an 8-year period at our institution: one after an unrelated marrow transplant in a toxoplasma-seronegative patient, the second complicating syngeneic marrow transplantation, and the third following allogeneic, related BMT. The disease is extremely rare in seronegative patients and

PH Chandrasekar; F Momin

1997-01-01

220

Graft-versus-leukemia effect of donor lymphocyte transfusions in marrow grafted patients  

Microsoft Academic Search

HE ROLE OF ALLOGENEIC lymphocytes in the eradi- cation of leukemia is well established. In mice, trans- plantation of allogeneic bone marrow eliminated leukemia, and transplantation of marrow from syngeneic donors failed.' Allogeneic marrow transplantation as a form of adoptive immunotherapy of leukemia was limited by the inevitable occurrence of graft-versus-host disease (GVHD).' Patients with GVHD had fewer relapses than

Hans-Jochem Kolb; A. V. M. B. Schattenberg; John M. Goldman; Bernd Hertenstein; Niels Jacobsen; William Arcese; Per Ljungman; Augustin Ferrant; Leo Verdonck; Dietger Niederwieser; Frits van Rhee; J. Mittermuehler; T. J. M. de Witte; E. Holler; H. Ansari

1995-01-01

221

Immune reconstitution after allogeneic marrow transplantation compared with blood stem cell transplantation  

Microsoft Academic Search

Allogeneic peripheral blood stem cell grafts contain about 10 times more T and B cells than marrow grafts. Because these cells may survive in transplant recipients for a long time, recipients of blood stem cells may be less immunocompromised than re- cipients of marrow. Immune reconstitution was studied in 115 patients randomly as- signed to receive either allogeneic marrow or

Jan Storek; Monja A. Dawson; Barry Storer; Terry Stevens-Ayers; David G. Maloney; Kieren A. Marr; Robert P. Witherspoon; William Bensinger; Mary E. D. Flowers; Paul Martin; Rainer Storb; Frederick R. Appelbaum; Michael Boeckh

2001-01-01

222

Marrow stromal cells for cellular cardiomyoplasty: Feasibility and potential clinical advantages  

Microsoft Academic Search

Objectives: Marrow stromal cells are mesenchymal stem cells able to differentiate into cardiomyocytes in vitro. We tested the hypothesis that marrow stromal cells, when implanted into myocardium, can undergo milieu-dependent differentiation and express cardiomyogenic phenotypes in vivo. Methods: Isogenic adult rats were used as donors and recipients to simulate autologous transplantation. Marrow stromal cells isolated from donor leg bones were

Jih-Shiuan Wang; Dominique Shum-Tim; Jacques Galipeau; Edgar Chedrawy; Nicoletta Eliopoulos; Ray C.-J. Chiu

2000-01-01

223

Depth of Subchondral Perforation Influences the Outcome of Bone Marrow Stimulation Cartilage Repair  

E-print Network

Depth of Subchondral Perforation Influences the Outcome of Bone Marrow Stimulation Cartilage Repair (wileyonlinelibrary.com). DOI 10.1002/jor.21386 ABSTRACT: Subchondral drilling and microfracture are bone marrow-based cartilage repair. Bone marrow stimulation can be achieved by abrasion arthroplasty,5 subchondral drilling

Buschmann, Michael

224

Chromosome aberrations in peripheral lymphocytes and radiation dose to active bone marrow in patients treated for cancer of the cervix  

SciTech Connect

An international study of cervical cancer patients reported a doubling of the risk for leukemia following radiotherapy. To evaluate the extent of residual chromosome damage in circulating T-cell lymphocytes in this population, approximately 200 metaphases were examined from each of 96 irradiated and 26 nonirradiated cervical cancer patients treated more than 17 years ago (average 23 years). Radiation dose averaged over the total red bone marrow was estimated to be 8.1 Gy. The type and frequency of stable and unstable chromosome aberrations were quantified in 24,117 metaphases. Unstable aberrations did not differ significantly between irradiated and nonirradiated patients (P greater than 0.5). Stable aberrations (i.e., translocations, inversions, or chromosomes with deleted segments), however, were significantly higher among irradiated (2.8 per 100 cells) compared to nonirradiated (0.7 per 100 cells) women (P less than 10(4)). The frequency of these stable aberrations was found to increase significantly with increasing dose to the bone marrow. These data indicate that a direct relationship between radiation dose and extent of damage to somatic cells persists in populations and can be detected many years after partial-body radiation exposure. The stable aberration rate in irradiated cervical cancer patients was 50 to 75% lower than those observed 25 years or more after radiation exposure in atomic bomb survivors and in ankylosing spondylitis patients treated with radiotherapy. The average marrow dose was only 1 Gy in the examined atomic bomb survivors and 3.5 Gy in the ankylosing spondylitis patients. It appears, then, that a very high dose delivered to the pelvic cavity in fractionated doses resulted in far fewer persistent stable aberrations than lower doses delivered either in acute whole-body exposure or in fractionated doses to the spinal column and sacroiliac joints.

Kleinerman, R.A.; Littlefield, L.G.; Tarone, R.E.; Machado, S.G.; Blettner, M.; Peters, L.J.; Boice, J.D. Jr. (National Cancer Institute, Bethesda, MD (USA))

1989-07-01

225

Red blood cells, multiple sickle cells (image)  

MedlinePLUS

Sickle cell anemia is an inherited disorder in which abnormal hemoglobin (the red pigment inside red blood cells) is produced. The abnormal hemoglobin causes red blood cells to assume a sickle shape, like the ones seen in this photomicrograph.

226

How red is the red autumn leaf herring and did it lose its red color?  

PubMed Central

The term “red herring” is commonly used to oppose a proposed hypothesis considered a logical fallacy. Pointing to problems in hypotheses is essential to science, and the use of the title red herring stimulates people to think. However, some hypotheses are later proven to be true, losing their red herring status. Others remain with the red herring tag.   A very famous and currently broadly accepted (sometimes with specific reservations) evolutionary signaling hypothesis that faced a very strong opposition was Zahavi's “handicap principle.”1 One of the most significant scientists in theoretical ecology and evolution, the late John Maynard Smith strongly opposed it,2 and only with the passing years gradually accepted it.3 Only 15 y later, when a mathematical solution for the problem was demonstrated,4 its status was changed. The case of Zahavi's handicap principle is an important lesson that the herring may not always be so red, but rather pink. PMID:22112457

Holopainen, Jarmo K.

2011-01-01

227

Bone marrow transplantation for Niemann-Pick Type IA disease  

Microsoft Academic Search

Summary Bone marrow transplantation has been undertaken with encouraging results as therapy for a wide variety of lysosomal storage diseases. We report a case of Niemann-Pick disease Type IA in which, despite the presence of only mild hypotonia with depressed reflexes, the clinical course of the disease appeared to be only slightly modified by this procedure, which was performed at

E. Bayever; N. Kamani; P. Ferreira; G. A. Machin; M. Yudkoff; K. Conard; M. Palmieri; J. Radcliffe; D. A. Wenger; C. S. AUGUSTI

1992-01-01

228

Treating Families of Bone Marrow Recipients and Donors  

ERIC Educational Resources Information Center

Luekemia and aplastic anemia are beginning to be treated by bone marrow transplants, involving donors and recipients from the same family. Such intimate involvement in the patient's life and death struggles typically produces a family crisis and frequent maladaptive responses by various family members. (Author)

Cohen, Marie; And Others

1977-01-01

229

Regulatory T lymphocytes in bone-marrow transplantation  

E-print Network

CD4+ CD25+ Regulatory T lymphocytes in bone-marrow transplantation Olivier Joffrea,1 & Joost P be the treatment of choice to prevent graft-versus-host disease and allograft rejection in transplantation medicine T lymphocytes. It appears therefore attractive to use the same mechanism to induce transplantation-tolerance. We

Paris-Sud XI, Université de

230

Effect of Rosiglitazone on Radiation Damage in Bone Marrow Hemopoiesis  

NASA Astrophysics Data System (ADS)

To study radiobiological effects and drugs, which can modify radiation injury, has an importance if we would like to avoid harmful effects of radiation due to emergency situations or treat patients with malignant diseases by radiotherapy. During the long treatment schedules patients may be treated by not only anticancer but many other drugs because of accompanying diseases. These drugs may also modify radiobiological effects. Rosiglitazone pre-treatment proved to be myeloprotective and accelerated recovery of 5-fluorouracil-damaged bone marrow in our previous experiments. Our new studies are designed to evaluate whether rosiglitazone has similar beneficial effects in radiation-damaged hemopoiesis. Bone marrow damage was precipitated by total body irradiation (TBI) using single increasing doses (2-10 Gy) of ?—irradiation in groups of mice. Lethality was well correlated with damage in hemopoiesis measured by cellularity of bone marrow (LD50 values were 4.8 and 5.3 gray respectively). Rosiglitazone, an insulin-sensitizing drug, had no significant effect on bone marrow cellularity. Insulin resistance associated with obesity or diabetes mellitus type 2 is intensively growing among cancer patients requiring some kind of radiotherapy. Therefore it is important to know whether drugs used for their therapy can modify radiation effects.

Benk?, Klára; Pintye, Éva; Szabó, Boglárka; Géresi, Krisztina; Megyeri, Attila; Benk?, Ilona

2008-12-01

231

Bone-marrow haematopoietic-stem-cell niches  

Microsoft Academic Search

Adult stem cells hold many promises for future clinical applications and regenerative medicine. The haematopoietic stem cell (HSC) is the best-characterized somatic stem cell so far, but in vitro expansion has been unsuccessful, limiting the future therapeutic potential of these cells. Here we review recent progress in characterizing the composition of the HSC bone-marrow microenvironment, known as the HSC niche.

Anne Wilson; Andreas Trumpp

2006-01-01

232

BONE MARROW MESENCHYMAL STEM CELLS ARE ABNORMAL IN MULTIPLE MYELOMA  

Microsoft Academic Search

Author contributions: CJ and MK performed the experiments and participated in the writing of the paper AM participated in the design of the research and the writing of the paper GM contributed to new analytical tools and gave technical assistance with the experiments HA collected bone marrow samples and clinical data FS gave technical assistance with the experiments DC and

Corre Jill; Mahtouk Karène; Attal Michel; Gadelorge Mélanie; Huynh Anne; Fleury Sandrine; Danho Clotaire; Laharrague Patrick; Klein Bernard; Rème Thierry

2007-01-01

233

Stem cells today: B1. Bone marrow stem cells  

Microsoft Academic Search

This review is the second in a series of four devoted to the analysis of recent studies on stem cells. The first considered embryo stem cells (ES). This review covers bone marrow stem cells. They are analysed initially in a historical perspective, and then in relation to foundation studies in the later 20th century before a detailed analysis is presented

RG Edwards

2004-01-01

234

Aplastic Anemia before Bone Marrow Transplantation and Antilymphocyte Globulin  

Microsoft Academic Search

In severe aplastic anemia, disease-dependent mortality was high before allogeneic bone marrow transplantation (BMT) and immunosuppressive therapies (IST) including antilymphocyte globulin became available. However, under supportive therapy alone, spontaneous remissions were observed in up to 20% of severe cases, reflecting the natural course of the disease. Therefore, in evaluating new forms of treatment, one has to keep in mind that

Hermann Heimpel

2000-01-01

235

Allogeneic bone marrow transplantation for lysosomal storage diseases  

Microsoft Academic Search

Patients with lysosomal storage disorders have visceral, skeletal, and neurological abnormalities and a limited life expectancy. Bone marrow transplantation has been used to correct the metabolic defects and leads to metabolic improvements in most patients However, the long-term effect of such therapy is uncertain. We analysed the data from 63 patients transplanted for lysosomal storage diseases. The transplant-related mortality was

P. M. Hoogerbrugge; O. F. Brouwer; P. Bordigoni; G. Cornu; P. Kapaun; J. J. Ortega; A. O'Meara; G. Souillet; D. Frappaz; S. Blanche; A. Fischer; O. Ringden

1995-01-01

236

Anaplasma platys in Bone Marrow Megakaryocytes of Young Dogs  

PubMed Central

Anaplasma platys is an obligate intracellular rickettsial pathogen that infects platelets of dogs, forming basophilic intracellular morulae. In the present report, cellular inclusions were documented in bone marrow thrombocyte precursors of two young naturally infected dogs, indicating that A. platys can infect megakaryocytes and promegakaryocytes. PMID:24622106

De Tommasi, A. Sara; Baneth, Gad; Breitschwerdt, Edward B.; Stanneck, Dorothee; Dantas-Torres, Filipe; Otranto, Domenico

2014-01-01

237

Bone Marrow Graft in Man after Conditioning by Antilymphocytic Serum  

Microsoft Academic Search

Allogeneic bone marrow grafts carried out after previous administration of antilymphocytic serum alone were attempted in 16 patients. Of these, six had acute myeloblastic leukaemia, four acute lymphoblastic leukaemia, and one a blast cell crisis in polycythaemia vera. Ten of these patients were in an overt phase of the disease and resistant to chemotherapy, while nine had complete agranulocytosis. In

G. Mathé; J. L. Amiel; L. Schwarzenberg; J. Choay; P. Trolard; M. Schneider; M. Hayat; J. R. Schlumberger; Cl. Jasmin

1970-01-01

238

Effect of Rosiglitazone on Radiation Damage in Bone Marrow Hemopoiesis  

SciTech Connect

To study radiobiological effects and drugs, which can modify radiation injury, has an importance if we would like to avoid harmful effects of radiation due to emergency situations or treat patients with malignant diseases by radiotherapy. During the long treatment schedules patients may be treated by not only anticancer but many other drugs because of accompanying diseases. These drugs may also modify radiobiological effects. Rosiglitazone pre-treatment proved to be myeloprotective and accelerated recovery of 5-fluorouracil-damaged bone marrow in our previous experiments. Our new studies are designed to evaluate whether rosiglitazone has similar beneficial effects in radiation-damaged hemopoiesis. Bone marrow damage was precipitated by total body irradiation (TBI) using single increasing doses (2-10 Gy) of {gamma}--irradiation in groups of mice. Lethality was well correlated with damage in hemopoiesis measured by cellularity of bone marrow (LD{sub 50} values were 4.8 and 5.3 gray respectively). Rosiglitazone, an insulin-sensitizing drug, had no significant effect on bone marrow cellularity. Insulin resistance associated with obesity or diabetes mellitus type 2 is intensively growing among cancer patients requiring some kind of radiotherapy. Therefore it is important to know whether drugs used for their therapy can modify radiation effects.

Benko', Klara [Euromedic Diagnostics Szeged LtD, Medical and Health Science Center, University of Debrecen (Hungary); Pintye, Eva [Department of Radiotherapy, Medical and Health Science Center, University of Debrecen (Hungary); Szabo, Boglarka; Geresi, Krisztina; Megyeri, Attila; Benko, Ilona [Department of Pharmacology and Pharmacotherapy, Medical and Health Science Center, University of Debrecen, Nagyerdei bld. 98., H-4032 Debrecen (Hungary)

2008-12-08

239

Bone Marrow Stromal Stem Cells: Nature, Biology, and Potential Applications  

Microsoft Academic Search

Bone marrow stromal cells are progenitors of skeletal tissue components such as bone, cartilage, the hemato- poiesis-supporting stroma, and adipocytes. In addition, they may be experimentally induced to undergo unortho- dox differentiation, possibly forming neural and myogenic cells. As such, they represent an important paradigm of post-natal nonhematopoietic stem cells, and an easy source for potential therapeutic use. Along with

Paolo Bianco; Mara Riminucci; Stan Gronthos; Pamela Gehron Robey

2001-01-01

240

Bone marrow transplantation in Schimke immuno-osseous dysplasia  

PubMed Central

Schimke immuno-osseous dysplasia (SIOD, OMIM 242900) is a rare autosomal recessive multisystem childhood disorder characterized by short stature, renal failure, T-cell immunodeficiency, and hypersensitivity to genotoxic agents. SIOD is associated with biallelic mutations in SMARCAL1 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily a-like 1), which encodes a DNA stress response enzyme with annealing helicase activity. Two features of SIOD causing much morbidity and mortality are bone marrow failure and T-cell deficiency with the consequent opportunistic infections. To address the safety and efficacy of bone marrow transplantation (BMT) in SIOD we reviewed the outcomes of the only five SIOD patients known to us in whom bone marrow or hematopoietic stem cell transplantation has been attempted. We find that only one patient survived the transplantation procedure and that the existing indicators of a good prognosis for bone marrow transplantation were not predictive in this small cohort. Given these observations, we also discuss some considerations for the poor outcomes. PMID:23950031

Baradaran-Heravi, Alireza; Lange, Jonas; Asakura, Yumi; Cochat, Pierre; Massella, Laura; Boerkoel, Cornelius F.

2013-01-01

241

Body/bone-marrow differential-temperature sensor  

NASA Technical Reports Server (NTRS)

Differential-temperature sensor developed to compare bone-marrow and body temperature in leukemia patients uses single stable amplifier to monitor temperature difference recorded by thermocouples. Errors are reduced by referencing temperatures to each other, not to separate calibration points.

Anselmo, V. J.; Berdahl, C. M.

1978-01-01

242

Outcome following late marrow relapse in childhood acute lymphoblastic leukemia  

SciTech Connect

Thirty-four children with acute lymphoblastic leukemia, who developed bone marrow relapse after treatment was electively stopped, received reinduction, consolidation, continuing therapy, and intrathecal (IT) methotrexate (MTX). Sixteen children who relapsed within six months of stopping treatment had a median second-remission duration of 26 weeks; all next relapses occurred in the bone marrow. In 18 children who relapsed later, the median duration of second remission was in excess of two years, but after a minimum of four years follow-up, 16 patients have so far relapsed again (six in the CNS). CNS relapse occurred as a next event in four of 17 children who received five IT MTX injections only and in two of 14 children who received additional regular IT MTX. Although children with late marrow relapses may achieve long second remissions, their long-term out-look is poor, and regular IT MTX does not afford adequate CNS prophylaxis. It remains to be seen whether more intensive chemotherapy, including high-dose chemoradiotherapy and bone marrow transplantation, will improve the prognosis in this group of patients.

Chessells, J.; Leiper, A.; Rogers, D.

1984-10-01

243

Massage Therapy for Patients Undergoing Autologous Bone Marrow Transplantation  

Microsoft Academic Search

The purpose of the current study was to examine the impact of massage therapy on psychological, physical, and psychophysiological measures in patients undergoing autologous bone marrow transplantation (BMT). Patients scheduled to undergo BMT were randomly assigned to receive either (a) massage therapy, consisting of 20-minute sessions of shoulder, neck, head, and facial massage, or (b) standard treatment. Overall effects of

Tim A. Ahles; Denise M. Tope; Briane Pinkson; Susan Walch; Danette Hann; Marie Whedon; Bradley Dain; Julia E. Weiss; Letha Mills; Peter M. Silberfarb

1999-01-01

244

Generation of Eosinophils from Cryopreserved Murine Bone Marrow Cells  

PubMed Central

Eosinophils are produced in the bone marrow from CD34+ eosinophil lineage–committed progenitors, whose levels in the bone marrow are elevated in a variety of human diseases. These findings suggest that increased eosinophil lineage–committed progenitor production is an important process in disease-associated eosinophilia. The pathways central to the biology of the eosinophil lineage–committed progenitor remain largely unknown. Thus, developing new methods to investigate the regulators of eosinophil lineage–committed progenitor differentiation is needed to identify potential therapeutic targets to specifically inhibit eosinophil production. We tested cytokine regimens to optimize liquid cultures for the study of eosinophil lineage–committed progenitor and eosinophil precursor differentiation into mature eosinophils. Stem cell factor (but not fms-related tyrosine kinase 3 ligand) was required for optimal yield of eosinophils. Furthermore, we evaluated the effects of cell preservation and scale on the culture, successfully culturing functional eosinophils from fresh and frozen murine bone marrow cells and in a standard-sized and 96-well culture format. In summary, we have developed an adaptable culture system that yields functionally competent eosinophils from murine low-density bone marrow cells and whose cytokine regime includes expansion of progenitors with stem cell factor alone with subsequent differentiation with interleukin 5. PMID:25551463

Schollaert, Kaila L.; Stephens, Michael R.; Gray, Jerilyn K.; Fulkerson, Patricia C.

2014-01-01

245

Mapping the Red Planet  

NASA Technical Reports Server (NTRS)

Since September 1997 the Mars Global Surveyor spacecraft has been orbiting the planet Mars and acquiring new data about the red planet that is changing our view of its present state and past history. Except for a few weeks in October 1997 and a few months in the Spring/Summer of 1998 when special science operations were conducted the spacecraft spent the first 18 months if its time at Mars getting to the right orbital geometry for the mapping mission. But on March 1, 1999 the MGS spacecraft trained its instruments onto the planet to begin a full Mars year (684 Earth days) of continuous systematic mapping and observation of the planet. The camera began wide angle and high resolution mapping, the thermal emission spectrometer began sensing the atmosphere and the material properties of the surface, the magnetometer searched out regions of abnormally high magnetism, the altimeter began determining the precise shape of the planet, and the radio science experiment began determining atmospheric pressures, temperatures and mapping the planet's gravity field. In a matter of a month more data was acquired about

Smith, David E.; Smith, David E.

2001-01-01

246

A Dosimetric Study of Radionuclide Therapy for Bone Marrow Ablation.  

NASA Astrophysics Data System (ADS)

In a phase I clinical trial, six multiple myeloma patients, who were non-responsive to conventional therapy and were scheduled for bone marrow transplantation, received Holmium-166 (166Ho) labeled to a bone seeking agent, DOTMP (1,4,7,10-tetraazacyclododecane -1,4,7,10-tetramethylene-phosphonic acid), for the purpose of bone marrow ablation. The specific aims of my research within this protocol were to evaluate the toxicity and efficacy of 166Ho DOTMP by quantifying the in vivo pharmacokinetics and radiation dosimetry, and by correlating these results to the biologic response observed. The reproducibility of pharmacokinetics from multiple injections of 166 Ho DOTMP administered to these myeloma patients was demonstrated from both blood and whole body retention. The skeletal concentration of 166 Ho DOTMP was heterogenous in all six patients: high in the ribs, pelvis, and lumbar vertebrae regions, and relatively low in the femurs, arms, and head. A novel technique was developed to calculate the radiation dose to the bone marrow in each skeletal ROI, and was applied to all six 166 Ho DOTMP patients. Radiation dose estimates for the bone marrow calculated using the standard MIRD "S" factors were compared with the average values derived from the heterogenous distribution of activity in the skeleton (i.e., the regional technique). The results from the two techniques were significantly different; the average of the dose estimates from the regional technique were typically 30% greater. Furthermore, the regional technique provided a range of radiation doses for the entire marrow volume, while the MIRD "S" factors only provided a single value. Dose volume histogram analysis of data from the regional technique indicated a range of dose estimates that varied by a factor of 10 between the high dose and low dose regions. Finally, the observed clinical response of cells and abnormal proteins measured in bone marrow aspirates and peripheral blood samples were compared with radiation dose estimates for the bone marrow calculated from the standard and regional technique. The results showed the regional technique values correlated more closely to several clinical response parameters. (Abstract shortened by UMI.).

Bayouth, John Ellis

247

CNTO 530 functions as a potent EPO mimetic via unique sustained effects on bone marrow proerythroblast pools.  

PubMed

Anemia as associated with numerous clinical conditions can be debilitating, but frequently can be treated via administration of epoetin-alfa, darbepoietin-alfa, or methoxy-PEG epoetin-beta. Despite the complexity of EPO-EPO receptor interactions, the development of interesting EPO mimetic peptides (EMPs) also has been possible. CNTO 530 is one such novel MIMETIBODY Fc-domain dimeric EMP fusion protein. In a mouse model, single-dose CNTO 530 (unlike epoetin-alfa or darbepoietin-alfa) bolstered red cell production for up to 1 month. In 5-fluorouracil and carboplatin-paclitaxel models, CNTO 530 also protected against anemia with unique efficiency. These actions were not fully accounted for by half-life estimates, and CNTO 530 signaling events therefore were studied. Within primary bone marrow erythroblasts, kinetics of STAT5, ERK, and AKT activation were similar for CNTO 530 and epoetin-alfa. p70S6K activation by CNTO 530, however, was selectively sustained. In vivo, CNTO 530 uniquely stimulated the enhanced formation of PODXL(high)CD71(high) (pro)erythroblasts at frequencies multifold above epoetin-alfa or darbepoietin-alfa. CNTO 530 moreover supported the sustained expansion of a bone marrow-resident Kit(neg)CD71(high)Ter119(neg) progenitor pool. Based on these distinct erythropoietic and EPOR signaling properties, CNTO 530 holds excellent promise as a new EPO mimetic. PMID:19264917

Sathyanarayana, Pradeep; Houde, Estelle; Marshall, Deborah; Volk, Amy; Makropoulos, Dorie; Emerson, Christine; Pradeep, Anamika; Bugelski, Peter J; Wojchowski, Don M

2009-05-14

248

CNTO 530 functions as a potent EPO mimetic via unique sustained effects on bone marrow proerythroblast pools  

PubMed Central

Anemia as associated with numerous clinical conditions can be debilitating, but frequently can be treated via administration of epoetin-alfa, darbepoietin-alfa, or methoxy-PEG epoetin-beta. Despite the complexity of EPO-EPO receptor interactions, the development of interesting EPO mimetic peptides (EMPs) also has been possible. CNTO 530 is one such novel MIMETIBODY Fc-domain dimeric EMP fusion protein. In a mouse model, single-dose CNTO 530 (unlike epoetin-alfa or darbepoietin-alfa) bolstered red cell production for up to 1 month. In 5-fluorouracil and carboplatin-paclitaxel models, CNTO 530 also protected against anemia with unique efficiency. These actions were not fully accounted for by half-life estimates, and CNTO 530 signaling events therefore were studied. Within primary bone marrow erythroblasts, kinetics of STAT5, ERK, and AKT activation were similar for CNTO 530 and epoetin-alfa. p70S6K activation by CNTO 530, however, was selectively sustained. In vivo, CNTO 530 uniquely stimulated the enhanced formation of PODXLhighCD71high (pro)erythroblasts at frequencies multifold above epoetin-alfa or darbepoietin-alfa. CNTO 530 moreover supported the sustained expansion of a bone marrow–resident KitnegCD71highTer119neg progenitor pool. Based on these distinct erythropoietic and EPOR signaling properties, CNTO 530 holds excellent promise as a new EPO mimetic. PMID:19264917

Sathyanarayana, Pradeep; Houde, Estelle; Marshall, Deborah; Volk, Amy; Makropoulos, Dorie; Emerson, Christine; Pradeep, Anamika; Bugelski, Peter J.

2009-01-01

249

HLA-A, -B, -DR haplotype frequencies from DNA typing data of 26,266 Chinese bone marrow donors.  

PubMed

HLA phenotypes of 26,266 Chinese individuals who were recruited as potential hematopoietic stem cell donors by the Shanghai Red Cross Marrow Donor Registry, part of the China Marrow Donor Program, were determined for HLA-A, -B, and -DRB1 alleles at low to intermediate resolution using DNA-based typing methods. The large sample size of the study allowed accurate calculation of the Chinese HLA haplotype frequencies. The observed alleles correspond to 19 HLA-A, 44 -B, and 13 -DR split antigens. The serologic equivalents of HLA-A36, -A80, -B78, and -DR18 alleles were not observed. A total of 2,241 distinct HLA-A, -B, -DRB1 haplotypes were identified. Three-locus haplotype frequency was estimated using the maximum likelihood method. The lowest haplotype frequency that can be reliably estimated at a 95% confidence level was 0.000057. Using this cutoff value, 1,220 haplotypes (54%) were statistically reliable and their cumulative haplotype frequency was 0.9730. The cumulative haplotype frequency of the remaining 1,021 haplotypes (46%) was 0.0270. A regression equation of p = 0.192 log N - 0.576 was derived to estimate the probability (p) of finding an HLA-A, -B, -DR split antigens-matched donor in a pool of N Chinese donors. PMID:17961775

Du, Ke-Ming; Ji, Yun; Xie, Jun-Hua; Fu, Min; Sun, Ying; Jin, Ye; Sun, Ji-Li; Yang, Jian-Hao; Zhang, Zheng; Mao, Zhen; Liu, Da-Zhuang; Qian, Kai-Cheng; Zhao, Tong-Mao

2007-10-01

250

International Federation of Red Cross and Red Crescent Societies  

NSDL National Science Digital Library

With a broad mission, the International Federation of Red Cross and Red Crescent Societies are involved in everything from disaster response and management to capacity building all over the globe. As one might expect, visitors to the site can learn about some of their more well-known programs in the "Get Involved" area on the homepage. Their homepage is also an excellent place to learn about some of their research publications, which include their annual "World Disasters Report" and their in-house magazine, "Red Cross, Red Crescent". For more nuts-and-bolts type information on the organization, visitors should browse through the "Who We Are", "What We Do", and "Where We Work" sections. Additionally, visitors can enter the "Our Programmes" section to learn about their various outreach efforts in different regions of the world.

251

Dose rate-dependent marrow toxicity of TBI in dogs and marrow sparing effect at high dose rate by dose fractionation  

Microsoft Academic Search

We evaluated the marrow toxicity of 200 and 300 cGy total-body irradiation (TBI) delivered at 10 and 60 cGy\\/min, respectively, in dogs not rescued by marrow transplant. Additionally, we compared toxicities after 300 cGy fractionated TBI (100 cGy fractions) to that after single-dose TBI at 10 and 60 cGy\\/min. Marrow toxicities were assessed on the basis of peripheral blood cell

Rainer Storb; Robert F Raff; Theodore Graham; Frederick R Appelbaum; H. Joachim Deeg; Friedrich G Schuening; George Sale; Kristy Seidel

1999-01-01

252

Stimulation tests for the bone marrow neutrophil pool in malignancies.  

PubMed

It has been known for decades that blood neutrophilia occurs after the administration of etiocholanolone, adrenocortical steroids, and endotoxins. Neutrophil leukocytosis in general may be due to several mechanisms such as increased stimulation of the myelopoiesis, increased release from the marrow, a shift from the marginated to the circulating pool (demargination), prolongation in the peripheral half-life, and decreased migration of neutrophils from the blood to the tissue. However, the principal cause of the neutrocytosis for each of the above mentioned agents is increased release of neutrophils from the bone marrow reserves. Since a sufficient reserve capacity is a prerequisite for optimal defenses against infections, the marrow response has been used to estimate the dose of chemotherapy expected to be tolerated without life-threatening neutropenia. However, none of the above "test substances" have gained widespread use due to adverse reactions or undesirable effects on neutrophil function. Recent progress in biotechnology has developed recombinant human (rh) hematopoietic growth factors ready for clinical use. Marrow myelopoiesis is stimulated by granulocyte colony-stimulating factor (rhG-CSF) and granulocyte-macrophage CSF (rhGM-CSF). The immediate effect, however, is mobilization of mature neutrophil granulocytes to the blood. Bone marrow cellularity seems to influence the neutrophil number mobilized during 24 hours by one subcutaneous injection of either rhG-CSF or rhGM-CSF. A recent pilot study has suggested such a "24 hour stimulation test" to predict severe neutropenia following cyclic chemotherapy. This concept is illustrated by two case reports. The "stimulation test" suggests that we may devise strategies to define patient subsets which may benefit from prophylactic growth factor administration during cyclic chemotherapy. PMID:7719232

Hansen, P B; Knudsen, L M; Johnsen, H E; Hansen, N E

1995-01-01

253

Allogeneic peripheral blood stem cell transplantation in patients with advanced hematologic malignancies: a retrospective comparison with marrow transplantation.  

PubMed

Allogeneic peripheral blood stem cell (PBSC) transplants from HLA-identical siblings were performed in 37 patients with advanced hematologic malignancies. Outcomes were compared to a historical group of 37 similar patients with advanced hematologic malignancies receiving bone marrow (BM) transplants from HLA-identical donors. The PBSC group and historical BM group were well matched for diagnosis, disease stage, age, and graft-versus-host disease (GVHD) prophylaxis. Patients received PBSC transplants between 1993 to 1995 while BM patients were treated between 1989 to 1994. Engraftment, measured by the time to reach a peripheral neutrophil count > 500/L and platelet count > 20,000/microL without transfusions, occurred on days 14 and 11 in the patients transplanted with PBSC compared to days 16 and 15 in the patients receiving BM (P = .00063, .00014). The PBSC group required a median of 8 U of red blood cells and 24 U of platelets compared to 17 U of red blood cells and 118 U of platelets for BM transplant recipients (P = .0005, .0001). The estimated risks of developing grades 2 to 4 acute GVHD were 37% for the PBSC group and 56% for the BM group (P = .18), while the estimated risks of grades 3 to 4 acute GVHD were 14% for the PBSC group and 33% for the BM group, P = .05). Chronic GVHD occurred in 7 of 18 evaluable patients receiving PBSC and 6 of 23 evaluable patients receiving BM, P = .5. The estimated risks of transplant-related mortality at 200 days were 27% versus 45% (P = .33) relapse were 70% versus 53% (P = .27) and of overall survival were 50% and 41% (P = .39) for patients transplanted with PBSC or BM, respectively. This retrospective comparison suggests that compared to marrow transplantation from HLA-identical donors, allogeneic PBSC transplantation from HLA-identical donors is associated with faster engraftment, fewer transfusions, and no greater incidence of acute or chronic GVHD. PMID:8839878

Bensinger, W I; Clift, R; Martin, P; Appelbaum, F R; Demirer, T; Gooley, T; Lilleby, K; Rowley, S; Sanders, J; Storb, R; Buckner, C D

1996-10-01

254

Effects of a hybrid micro/nanorod topography-modified titanium implant on adhesion and osteogenic differentiation in rat bone marrow mesenchymal stem cells  

PubMed Central

Background and methods Various methods have been used to modify titanium implant surfaces with the aim of achieving better osseointegration. In this study, we fabricated a clustered nanorod structure on an acid-etched, microstructured titanium plate surface using hydrogen peroxide. We also evaluated biofunctionalization of the hybrid micro/nanorod topography on rat bone marrow mesenchymal stem cells. Scanning electron microscopy and x-ray diffraction were used to investigate the surface topography and phase composition of the modified titanium plate. Rat bone marrow mesenchymal stem cells were cultured and seeded on the plate. The adhesion ability of the cells was then assayed by cell counting at one, 4, and 24 hours after cell seeding, and expression of adhesion-related protein integrin ?1 was detected by immunofluorescence. In addition, a polymerase chain reaction assay, alkaline phosphatase and Alizarin Red S staining assays, and osteopontin and osteocalcin immunofluorescence analyses were used to evaluate the osteogenic differentiation behavior of the cells. Results The hybrid micro/nanoscale texture formed on the titanium surface enhanced the initial adhesion activity of the rat bone marrow mesenchymal stem cells. Importantly, the hierarchical structure promoted osteogenic differentiation of these cells. Conclusion This study suggests that a hybrid micro/nanorod topography on a titanium surface fabricated by treatment with hydrogen peroxide followed by acid etching might facilitate osseointegration of a titanium implant in vivo. PMID:23345973

Zhang, Wenjie; Li, Zihui; Huang, Qingfeng; Xu, Ling; Li, Jinhua; Jin, Yuqin; Wang, Guifang; Liu, Xuanyong; Jiang, Xinquan

2013-01-01

255

Determination of effective rAAV-mediated gene transfer conditions to support chondrogenic differentiation processes in human primary bone marrow aspirates.  

PubMed

The genetic modification of freshly aspirated bone marrow may provide convenient tools to enhance the regenerative capacities of cartilage defects compared with the complex manipulation of isolated progenitor cells. In the present study, we examined the ability and safety of recombinant adeno-associated virus (rAAV) serotype 2 vectors to deliver various reporter gene sequences in primary human bone marrow aspirates over time without altering the chondrogenic processes in the samples. The results demonstrate that successful rAAV-mediated gene transfer and expression of the lacZ and red fluorescent protein marker genes were achieved in transduced aspirates at very high efficiencies (90-94%) and over extended periods of time (up to 125 days) upon treatment with hirudin, an alternative anticoagulant that does not prevent the adsorption of the rAAV-2 particles at the surface of their targets compared with heparin. Application of rAAV was safe, displaying neither cytotoxic nor detrimental effects on the cellular and proliferative activities or on the chondrogenic processes in the aspirates especially using an optimal dose of 0.5?mg?ml(-1) hirudin, and application of the potent SOX9 transcription factor even enhanced these processes while counteracting hypertrophic differentiation. The current findings demonstrate the clinical value of this class of vector to durably and safely modify bone marrow aspirates as a means to further develop convenient therapeutic approaches to improve the healing of cartilage defects. PMID:25338919

Rey-Rico, A; Frisch, J; Venkatesan, J K; Schmitt, G; Madry, H; Cucchiarini, M

2015-01-01

256

Intrathoracic extramedullary hematopoiesis: appearance on /sup 99m/Tc sulfur colloid marrow scan  

SciTech Connect

Imaging of the bone marrow by radionuclide scanning was performed using colloids, which are phagocytized by the reticuloendothelial cells of the marrow, or radioiron, which is incorporated into reticulocytes. The use of the former radiopharmaceutical is based on the assumption, generally valid except in aplastic states or after irradiation, that the distribution of hematopoietic and reticuloendothelial tissue in the marrow is similar. Regardless of the method used, active adult marrow is normally distributed only in the axial skeleton and proximal humeri and femurs. Marrow imaging has been used in the evaluation of myeloproliferative disorders, leukemia, lymphoma, aplastic states, malignancy metastatic to marrow, and hemolytic anemia. We report a case of thalassemia major in which the diagnosis of intrathoracic extramedullary hematopoiesis was confirmed with the /sup 99m/Tc sulfur colloid bone marrow scan.

Bronn, L.J.; Paquelet, J.R.; Tetalman, M.R.

1980-06-01

257

Red Tide off Texas Coast  

NASA Technical Reports Server (NTRS)

Red tides (algae) bloomed late this summer along a 300-mile stretch of Texas' Gulf Coast, killing millions of fish and shellfish as well as making some people sick. State officials are calling this the worst red tide bloom in 14 years. The algae produces a poison that paralyzes fish and prevents them from breathing. There is concern that the deadly algae could impact or even wipe out this year's oyster harvest in Texas, which usually peaks during the Thanksgiving and Christmas holidays. The red tides were first observed off the Texas coast in mid-August and have been growing steadily in size ever since. Red tides tend to bloom and subside rapidly, depending upon changes in wind speed and direction, water temperature, salinity, and rainfall patterns (as the algae doesn't do as well in fresher water). This true-color image of the Texas Gulf Coast was acquired on September 29, 2000, by the Moderate-resolution Imaging Spectroradiometer (MODIS) flying aboard NASA's Terra spacecraft. The red tide can be seen as the dark reddish discoloration in the ocean running southwest to northeast along the coast. In this scene, the bloom appears to be concentrated north and east of Corpus Christi, just off Matagorda Island. The image was made at 500-meter resolution using a combination of MODIS' visible bands 1 (red), 4 (green), and 3 (blue). The city of Houston can be seen clearly as the large, greyish cluster of pixels to the north and west of Galveston Bay, which is about mid-way up the coastline in this image. Also visible in this image are plumes of smoke, perhaps wildfires, both to the north and northeast of Houston. For more information about red tides, refer to the Texas Red Tide Web site. Image courtesy Andrey Savtchenko, MODIS Data Support Team, and the MODIS Ocean Team, NASA's Goddard Space Flight Center

2002-01-01

258

Red Tide and Shellfish Poisoning  

NSDL National Science Digital Library

This EnviroFacts informational page discusses how red tide develops and how people might be affected by this phenomenon. It covers physical damage, oxygen depletion, direct poisoning, and indirect poisoning including paralytic shellfish poisoning (PSP), diarrhetic shellfish poisoning (DSP), neurotoxic shellfish poisoning (NSP), amnesiac shellfish poisoning (ASP), and aerosol toxins. The page concludes with a discussion of red tide and the role of the sea fisheries research institute.

Maneveldt, Gavin W.

259

Analytic Evaluation of RED Performance  

Microsoft Academic Search

End-to-end congestion control mechanisms such as those inTCP are not enough to prevent congestion collapse in the Internet (forstarters, not all applications might be willing to use them), and they mustbe supplemented by control mechanisms inside the network. The IRTFhas singled out Random Early Detection (RED) as one queue managementscheme recommended for rapid deployment throughout the Internet. However,RED is not

Thomas Bonald; Martin May; Jean-chrysostome Bolot

2000-01-01

260

Red face and fungi infection.  

PubMed

Red face syndrome is characterized by an erythematous dermatitis that is produced by different entities. These include rosacea, seborrheic dermatitis, contact dermatitis, atopic dermatitis, psoriasis, cutaneous lupus, photodermatosis, post-topical steroid dermatosis, demodicosis, borderline borderline (BB) leprosy, mastocytosis, carcinoid, postneoplasia flushing, cutaneous lymphoma, tineas, ulerythema ophryogenes, and psychosomatic flushing. Red face is a relatively common dermatologic manifestation. Our goal is to review tinea corporis and other fungi that affect this region causing facial erythema and its therapeutic management. PMID:25441465

Welsh, Oliverio; Vera-Cabrera, Lucio

2014-01-01

261

Sex and the Red Queen  

Microsoft Academic Search

\\u000a Negative frequency-dependent selection exerted by parasites and pathogens can generate a selective advantage for rare host\\u000a genotypes. This mechanism, known as the Red Queen, is currently considered to be one of the most likely explanations for the\\u000a predominance of sexual reproduction in natural populations. Even so, the extent to which the Red Queen can and does provide\\u000a an advantage to

Maurine Neiman; Britt Koskella

262

An assessment of bone marrow and bone endosteum dosimetry methods for photon sources  

NASA Astrophysics Data System (ADS)

The rather complex and microscopic histological structure of the skeletal system generally limits one's ability to accurately model this tissue during dosimetric evaluations. Consequently, various assumptions must be made to evaluate the absorbed dose from external and internal photons to the radiosensitive tissues of the red (or haematopoietically active) bone marrow and the osteogenic tissues of the skeletal endosteum. These various methods for photon skeletal dosimetry have not been inter-compared, partly due to the lack of a realistic reference model that can provide a high-resolution three-dimensional geometry for secondary electron particle transport. In the present study, the paired-image radiation transport (PIRT) model developed by Shah et al (2005 J. Nucl. Med. 45 344) was utilized to evaluate the absorbed dose per incident photon fluence to these skeletal regions from idealized parallel beams of monoenergetic photons. The PIRT model results were then used as a local reference against which absorbed doses via other methods were compared. For red bone marrow dosimetry, four approximate techniques were considered: (1) the dose response function method (DRF method) presented in ORNL/TM-8381, (2) the mass-energy absorption coefficient ratio method (two-parameter MEAC method), (3) the MEAC method with the additional use of energy-dependent dose enhancement factors from King and Spiers (1985 Br. J. Radiol. 58 345) (three-parameter MEAC method), and (4) the three-parameter MEAC method applied at the voxel level through the use image-specific CT numbers (CTN method). For the bone endosteum (i.e., bone surfaces), two approximate techniques were compared: (1) the DRF method for bone surfaces and (2) the homogeneous bone approximation (HBA) method. In each case, the local reference standard was assumed to be that of the PIRT model. Four different ex vivo bone specimens with distinctively different internal structures were used in the study: the cranium, the lumbar vertebra, the os coxae and the left middle rib, each excised from a 66 year male cadaver (body mass index, 22.7 kg m-2). High-resolution CT images of these skeletal sites were used to construct computational voxel models for Monte Carlo radiation transport. Study results indicated that skeletal sites with thick cortical regions and thick trabeculae such as in the cranium provide considerable beam attenuation at low photon energies, which is not properly accounted for in methods based on a homogeneous skeletal tissue structure (DRF, MEAC, HBA). For bone marrow dose assessment, the CTN method showed the best agreement with PIRT model results over a broad range of photon energies, while the HBA method showed better agreement with the PIRT model in assessing bone endosteum dose at energies above 100 keV. Bone surface doses were better approximately by the DRF method at energies below 50 keV. Considerable secondary electron escape at photon energies over 1-3 MeV were accounted for in RBM dose assessment only in the PIRT model, as the other methods presume either an infinite expanse of spongiosa (DRF) or the existence of charge-particle equilibrium (MEAC, CTN).

Lee, Choonik; Lee, Choonsik; Shah, Amish P.; Bolch, Wesley E.

2006-11-01

263

{delta}-ALAD activity variations in red blood cells in response to lead accumulation in rock doves (Columba livia)  

SciTech Connect

The enzyme {delta}-aminolevulinic acid dehydratase ({delta}-ALAD, E.C. 4.2.1.24), catalyses the second step of the haeme biosynthetic pathway and is required to maintain the haemoglobin and cytochrome content in red cells. {delta}-ALAD is not only found in bone marrow cells, the major site of haeme synthesis, but also in circulating erythrocytes and other tissues. An inverse correlation was found between {delta}-ALAD activity in red blood cells and lead concentration in the blood. The degree of {delta}-ALAD inhibition in erythrocytes has been widely accepted as a standard bioassay to detect acute and chronic lead exposure in humans and in avians. The value of this parameter as an indicator for environmental lead has been often reported in doves and Scanlon. In lead-treated rats, an increase in {delta}-ALAD activity in bone marrow cells and in blood samples was shown by radioimmunoassay at 5 and 9 days after the treatment. Similarly, the amount of {delta}-ALAD seems to be more sensitive to lead in avian species than in mammals, the usefulness of blood {delta}-ALAD activity as an index of lead exposure has already been questioned by Hutton in the pigeon and by Jaffe et al. in humans. The present investigation studied the toxic effects of lead on rock dove red blood cell {delta}-ALAD activity in two situations: in doves treated with lead acetate in the laboratory and in doves exposed to the environment of Alcala de Henares. The final lead blood concentrations were lower in the environmental than in the laboratory doves. {delta}-ALAD activity in bone marrow cells and the relationships between lead accumulation and enzyme activity in red cells, are examined. 20 refs., 5 figs., 1 tab.

Gonzalez, M.; Tejedor, M.C. [Universidad de Alcala de Henares (Spain)

1992-10-01

264

Recognization of receptors on bone marrow-derived dendritic cells bound with Pholiota nameko polysaccharides.  

PubMed

Three major active polysaccharides isolated from Pholiota nameko (PNPS), including PNPS-1, PNPS-2 and PNPS-3, had been proved to inhibit the maturation of the murine bone marrow-derived dendritic cells (BMDCs). This paper recognized the affinity bind between PNPS and the five receptors (TLR2, TLR4, CD14, Dectin-1 and Mannose receptor) on BMDCs, using the bio-layer interferometry (BLI)-based biosensor technology developed by ForteBio on Octet RED system (Fortebio, Inc.). From the primary binding experiment, the gradient binding experiment and the inhibition binding experiment between the receptor proteins and PNPS, combined with the binding experiment between PNPS and the BMDCs membranes, we found that PNPS-1, PNPS-2 and PNPS-3 presented strong affinity bind with both TLR2 and Dectin-1 on BMDCs, only PNPS-3 with Mannose receptor. These data confirmed that PNPS could interact with TLR2, Dectin-1 and Mannose receptor that were very important for the affinity bind of these receptors and PNPS, which triggered the further stimulation on BMDCs. PMID:25158288

Li, Haiping; Tao, Yongqing; Zhao, Pei; Ban, Xiaopan; Zhi, Dexian; Li, Guoliang; Wang, Fenlin; Yang, Xiaoli; Huai, Lihua

2015-01-01

265

High-Frequency Vibration Treatment of Human Bone Marrow Stromal Cells Increases Differentiation toward Bone Tissue  

PubMed Central

In order to verify whether differentiation of adult stem cells toward bone tissue is promoted by high-frequency vibration (HFV), bone marrow stromal cells (BMSCs) were mechanically stimulated with HFV (30?Hz) for 45 minutes a day for 21 or 40 days. Cells were seeded in osteogenic medium, which enhances differentiation towards bone tissue. The effects of the mechanical treatment on differentiation were measured by Alizarin Red test, (q) real-time PCR, and protein content of the extracellular matrix. In addition, we analyzed the proliferation rate and apoptosis of BMSC subjected to mechanical stimulation. A strong increase in all parameters characterizing differentiation was observed. Deposition of calcium was almost double in the treated samples; the expression of genes involved in later differentiation was significantly increased and protein content was higher for all osteogenic proteins. Lastly, proliferation results indicated that stimulated BMSCs have a decreased growth rate in comparison with controls, but both treated and untreated cells do not enter the apoptosis process. These findings could reduce the gap between research and clinical application for bone substitutes derived from patient cells by improving the differentiation protocol for autologous cells and a further implant of the bone graft into the patient. PMID:23585968

Prè, D.; Ceccarelli, G.; Visai, L.; Benedetti, L.; Imbriani, M.; Cusella De Angelis, M. G.; Magenes, G.

2013-01-01

266

[Effect of bone marrow cells transplantation on the decidua formation in pseudopregnant rats].  

PubMed

One of the most common causes of the current pregnancy loss is the failure of the decidual reaction of endometrial cells. It is assumed that a partial source of decidual cells in endometrial tissue is bone marrow cells (BMCs). In the present work, we have studied possible effect of BMCs transplantation on the process of decidualization using the model of pseudopregnancy in rats. BMCs were flushed from the rat femurs and tibias. The obtained suspension of single BMCs was injected into one of rat uterine horns on the 5th day of pseudopregnancy. PBS without cells was injected into the contralateral horn served as the control. Rats were sacrificed on the 11th day of pseudopregnancy. Decidua formed in the experimental uterine horn showed an increase in the meso-antimezometral direction of their diameter of about 1.5-2 times as compared with a control horn. The weight of decidual tissue in the experimental horn exceeded 3 times the weight of the control one. The presence of transplanted BMCs in decidual tissue was documented by preliminary double staining of BMCs with membrane dye PKH 26 Red and nuclear dye Hoechst 33342. Histological analysis of decidua sections after transplantation revealed any alterations neither in cell differentiation nor in tissue structure. We conclude that BMCs transplantation stimulates decidualization in animals. PMID:25509160

Domnina, A P; Mikha?lov, V M; Nikol'ski?, N N

2014-01-01

267

Plasmodium falciparum transmission stages accumulate in the human bone marrow  

PubMed Central

Transmission of Plasmodium falciparum malaria parasites requires formation and development of gametocytes, yet all but the most mature of these sexual parasite forms are absent from the blood circulation. We performed a systematic organ survey in pediatric cases of fatal malaria to characterize the spatial dynamics of gametocyte development in the human host. Histological studies revealed a niche in the extravascular space of the human bone marrow where gametocytes formed in erythroid precursor cells and underwent development before reentering the circulation. Accumulation of gametocytes in the hematopoietic system of human bone marrow did not rely on cytoadherence to the vasculature as does sequestration of asexual-stage parasites. This suggests a different mechanism for the sequestration of gametocytes that could potentially be exploited to block malaria transmission. PMID:25009232

Joice, Regina; Nilsson, Sandra K.; Montgomery, Jacqui; Dankwa, Selasi; Egan, Elizabeth; Morahan, Belinda; Seydel, Karl B.; Bertuccini, Lucia; Alano, Pietro; Williamson, Kim C.; Duraisingh, Manoj T.; Taylor, Terrie E.; Milner, Danny A.; Marti, Matthias

2014-01-01

268

Cardiovascular repair with bone marrow-derived cells  

PubMed Central

While bone marrow (BM)-derived cells have been comprehensively studied for their propitious pre-clinical results, clinical trials have shown controversial outcomes. Unlike previously acknowledged, more recent studies have now confirmed that humoral and paracrine effects are the key mechanisms for tissue regeneration and functional recovery, instead of transdifferentiation of BM-derived cells into cardiovascular tissues. The progression of the understanding of BM-derived cells has further led to exploring efficient methods to isolate and obtain, without mobilization, sufficient number of cell populations that would eventually have a higher therapeutic potential. As such, hematopoietic CD31+ cells, prevalent in both bone marrow and peripheral blood, have been discovered, in recent studies, to have angiogenic and vasculogenic activities and to show strong potential for therapeutic neovascularization in ischemic tissues. This article will discuss recent advancement on BM-derived cell therapy and the implication of newly discovered CD31+ cells. PMID:23826576

Kim, Woan-Sang; Lee, Sangho

2013-01-01

269

Bone marrow transplantation after the Chernobyl nuclear accident  

Microsoft Academic Search

On April 26, 1986, an accident at the Chernobyl nuclear power station in the Soviet Union exposed about 200 people to large doses of total-body radiation. Thirteen persons exposed to estimated total-body doses of 5.6 to 13.4 Gy received bone marrow transplants. Two transplant recipients, who received estimated doses of radiation of 5.6 and 8.7 Gy, are alive more than

A. Baranov; R. P. Gale; A. Guskova; E. Piatkin; G. Selidovkin; L. Muravyova; R. E. Champlin; N. Danilova; L. Yevseeva; L. Petrosyan

1989-01-01

270

The mechanism of antibody formation in mouse bone marrow  

Microsoft Academic Search

The bone marrow (BM) is the major production site of B lymphocytes.\\u000aThe newly formed small B lymphocytes migrate, after a maturation period\\u000aof one or more days, to the peripheral lymphoid organs (spleen, lymph\\u000anodes, tonsils, etc.), where they can be activated by antigen (after\\u000awhich they can differentiate into antibody-forming cells) or die.\\u000aAccording to this view, most

G. Koch

1982-01-01

271

Lentiviral tracking of vascular differentiation in bone marrow progenitor cells  

Microsoft Academic Search

Lentiviral vectors encoding for identifiable marker genes controlled by lineage-specific promoters can be used to track differentiation of bone marrow progenitors into endothelial cells and\\/or smooth muscle cells. Human VE-Cadherin and Smoothelin-B promoters were cloned into a self-inactivating lentiviral vector (HR-VECad and HR-SMTHB) and used to drive expression of green fluorescent protein (eGFP). These constructs demonstrated specific promoter activity in

Jeffrey Schmeckpeper; Yasuhiro Ikeda; Arun H. S. Kumar; Pat Metharom; Stephen J. Russell; Noel M. Caplice

2009-01-01

272

Solutions to Avoid Red Tide, Inc.  

NSDL National Science Digital Library

This "Solutions to Avoid Red Tide" (START) webpage offers basic information and videos about red tide, effects of red tide, and research being done regarding red tide. START is a grassroots, non-profit organization based in Florida. Their mission is to keep federal, state, and local officials aware of red tide and to get these officials to fund projects to remediate and/or prevent the problem. Information on how to join or help START is also provided.

Solutions to Avoid Red Tide, Inc.

273

Red cell metabolism studies on Skylab  

NASA Technical Reports Server (NTRS)

Blood samples from Spacelab crewmembers were studied for possible environment effects on red cell components. Analysis involved peroxidation of red cell lipids, enzymes of red cell metabolism, and levels of 2,3-diphosphoglyceric acid and adenosine triphosphate. Results show that there is no evidence of lipid peroxidation, that biochemical effect known to be associated with irreversible red cell damage. Changes observed in glycolytic intermediates and enzymes cannot be directly implicated as indicating evidence of red cell damage.

Mengel, C. E.

1977-01-01

274

[In vitro activity of human bone marrow cells after cryopreservation in liquid nitrogen for 21 - 25 years].  

PubMed

The aim of this study was to investigate the best method to preserve human bone marrow cells and the effectiveness of long term cryopreservation at -80 degrees C. The human bone marrow cells in 20 samples were firstly frozen by a programmed freezer or -80 degrees C refrigerator, and then were preserved in liquid nitrogen with DMSO-AuP (10% dimethylsulfonamide, 10% autologous plasma) or DMSO-HES-HuA (5% dimethylsulfonamide, 6% hydroxyethyl starch, 4% human serum albumin) as cryoprotectant for 21 to 25 years. They were thawed in 38 degrees C. The cell sample frozen in -80 degrees C refrigerator was frozen at a low frozen speed of 1 degrees C/min which was the same as the programmed freezer before -30 degrees C. Before detection the bone marrow cells were taken from liquid nitrogen and were thawed in 38 degrees C, then the suspension of bone marrow cells was prepared for detection. The cell morphology and recovery rate of erythrocytes, nucleocytes and platelets; the recovery rate of hematopoietic stem progenitors cells, as well as mesenchymal stem cells were determined. The results showed that the protective effectiveness of DMSO-HES-HuA was better than DMSO-AuP. The mature erythrocytes were destroyed lightly [(3.5 +/- 1.5)% versus (12.6 +/- 4.8)%], the hemolysis rate was lower [(3.3 +/- 1.6)% versus (23.1 +/- 5.1)%]. Osmotic fragility of erythrocytes in the former was not changed, but was dropped in the latter. The recovery rates of red cell, platelet, granulocyte-macrophage colony forming units and long term culture-initiating cells were higher in the former than that in the latter [(96.1 +/- 1.8)%, (70.0 +/- 9.5)%, (49.2 +/- 10.9)%, (54.2 +/- 13.8)% versus (76.3 +/- 5.6)%, (52.7 +/- 8.1)%, (43.5 +/- 12.3)%, (47.2 +/- 13.6)% respectively]. With each kind of cryoprotectant or frozen method, the frozen MSC could keep the original growth properties. With the same cryoprotectant and different frozen method, the cryopreservative effectiveness was not different. The influence of the cryoprotectant prescriptions and the frozen methods on the cryopreservative effectiveness was little. It is concluded that the human bone marrow cells with DMSO-AuP or DMSO-HES-HuA as cryoprotectant, frozen by a programmed freezer or -80 degrees C refrigerator, could be then preserved in liquid nitrogen for long time. When the preserving time was as long as 21 to 25 years, the morphology, the recovery rate and the activity of various kinds of cells were still good. The method of freezing by -80 degrees C refrigerator with 5% DMSO-6% HES-4% HuA and preserving in liquid nitrogen would be convenient, cheap and easily-manipulated for preservation of the human bone marrow cells. PMID:20137152

Huang, You-Zhang; Shen, Jian-Liang; Gong, Li-Zhong; Zheng, Pei-Hao; Liu, Yi; Yin, Wen-Jie; Cen, Jian; Wang, Ning; Zhao, De-Feng

2010-02-01

275

Functional capacity of marginated and bone marrow reserve granulocytes.  

PubMed

Marginated and bone marrow reserve granulocytes were obtained from young healthy volunteers after subcutaneous administration of aqueous epinephrine (0.4 ml/m2) or intravenous administration of hydrocortisone sodium succinate (250 mg), respectively. These leukocytes were compared with circulating granulocytes for the ability to adhere to surfaces, migrate in a random fashion, respond to chemoattractants, interact with autologous serum opsonins, and phagocytize and kill five common bacterial pathogens. As contrasted with circulating neutrophils, marginated cells had enhanced phagocytic and killing capacity for some pathogens, whereas adherence, random migration, chemotaxis, and chemiluminescence for the two cell populations were equivalent. Bone marrow reserve cells demonstrated increased activity for three functional mechanisms; chemotaxis for these cells averaged 21% higher than that for circulating cells, and phagocytosis was 32% higher, with 6 to 17% greater killing of the five bacterial species studied. All of these differences were statistically significant (P less than 0.05). Random migration and interaction with serum opsonins were unchanged in bone marrow granulocytes. These enhanced functional properties of neutrophils which are outside of the circulating pool may represent important host defense mechanisms during episodes of bacterial infection. PMID:3653982

Steele, R W; Steele, C R; Pilkington, N S; Charlton, R K

1987-10-01

276

Breast Cancer Studies Raise Doubts Over Bone Marrow Transplants  

NSDL National Science Digital Library

On April 15, the National Cancer Institute (NCI) and the American Society of Clinical Oncology (ASCO) released long-awaited preliminary results of five studies on bone marrow transplants for women with advanced breast cancer. Considered a last resort, the painful and costly procedure involves ultrahigh doses of chemotherapy which destroy the patient's bone marrow which must then be replaced by a transplant or marrow-restoring stem cells. Four of the five studies found no difference in survival rates of patients who had high-dose chemotherapy with transplants, and those who had lower doses of chemotherapy. The fifth study, from South Africa, did find a benefit for women with positive lymph nodes. Critics of the transplant therapy argue that the studies show thousands of women have needlessly undergone excruciating and expensive procedures. However, because the five studies are so different, attacking tumors in different ways and involving women in different stages of breast cancer, the NCI and others have asserted that this is far from the last word on the subject, and the NCI has plans to fund fifteen additional studies. The sites listed provide information about breast cancer treatments.

de Nie, Michael Willem.

1999-01-01

277

T2 vertebral bone marrow changes after space flight  

NASA Technical Reports Server (NTRS)

Bone biopsies indicate that during immobilization bone marrow adipose tissue increases while the functional cellular fraction decreases. One objective of our Spacelab flight experiment was to determine, using in vivo volume-localized magnetic resonance spectroscopy (VLMRS), whether bone marrow composition was altered by space flight. Four crew members of a 17 day Spacelab mission participated in the experiment. The apparent cellular fraction and transverse relaxation time (T2) were determined twice before launch and at several times after flight. Immediately after flight, no significant change in the cellular fraction was found. However, the T2 of the cellular, but not the fat component increased following flight, although to a variable extent, in all crew members with a time course for return to baseline lasting several months. The T2 of seven control subjects showed no significant change. Although these observations may have several explanations, it is speculated that the observed T2 changes might reflect increased marrow osteoblastic activity during recovery from space flight.

LeBlanc, A.; Lin, C.; Evans, H.; Shackelford, L.; Martin, C.; Hedrick, T.

1999-01-01

278

Zinc enhances bone metabolism in ovariectomized rats and exerts anabolic osteoblastic/adipocytic marrow effects ex vivo.  

PubMed

Investigations of bone mass and marrow adiposity are critical for defining the role of zinc (Zn) in bone metabolism. Rats used for study were grouped as follows: control (sham), ovariectomy (OVX), ovariectomy + estradiol (OVX-E), ovariectomy + Zn treatment (OVX-Zn). Bone mineral density (BMD) was quantified (microCT); serum osteocalcin, adiponectin, RANKL, and TRAP levels were assayed (ELISA); and biochemical determinations of serum alkaline phosphatase (ALP), calcium (Ca), and phosphorus (P) were done. Cells derived from bone mesenchymal stem cell (BMSC) isolates of respective test groups were compared, identifying primary osteoblasts by MTT assay and adipocytes by Oil Red O stain. Osteocalcin and adiponectin levels in culture supernatants were determined by ELISA. Zn supplementation resulted in a modest increase in BMD, but serum osteocalcin and ALP activity increased significantly (P?Red O uptake and adiponectin level in supernatant were sharply diminished in cultured OVX-Zn (vs OVX) cells (P?

Li, Binbin; Liu, Hao; Jia, Shengnan

2015-02-01

279

Growth declines in red spruce  

SciTech Connect

In this letter, the authors take issue with Zedaker, Hyink, and Smith who have indicated that observed red spruce growth declines can be expected based on growth trends for even-aged stands of red spruce as documented in Meyer (1929). Recently, an examination was made of stand stocking levels at 750 sites where red spruce were cored and neither the rate of growth decline nor the extent of mortality were found to be related to stand stocking levels or previous disturbance history. The authors conclude that the Meyer data do not represent an appropriate model for stand dynamics of old-growth, high-elevation stands and no not adequately explain the growth declines observed at many of those sites.

McLaughlin, S.B. (Oak Ridge National Lab., TN (USA)); Adams, H.S. (Lancaster Community College, Clifton Forge, VA (USA))

1987-10-01

280

UBVRI photometry of red stars  

NASA Astrophysics Data System (ADS)

A total of 50 Mira- and SR-type red variable stars were observed by means of the photoelectric photometry UBVRI Kron-Cousins system. In addition, 15 nearby red dwarf stars having spectral subtypes similar to those of Mira stars at maximum were observed in order to show that the conversion of the natural system into the Landolt (1983) standard system can be made for stars as red as the Mira variables, in spite of the shortage of standard late M-type stars. The relationship function and spectral type-color index scale on the Johnson system was converted into the present system. By means of VRI photometry, the spectral subtype can immediately be determined in different phases of the light curve. SR variables have the same color indices and spectral subclasses as Mira variables.

Celis S., L.

1986-03-01

281

Jupiter's Red Spot Jr. Lithograph  

NSDL National Science Digital Library

This lithograph traces the emergence and color change of an Earth-sized storm on Jupiter. The storm appears to be the same color as Jupiter's trademark Great Red Spot and has been dubbed Red Spot Jr. In the accompanying educational activity, In Search of...Monster Storms, students compare Jupiter's storms with those of Earth through a level 1 inquiry activity using the images and text from the lithograph and other resources. A level 1 inquiry activity can help prepare students to become independent thinkers.

2012-12-06

282

Red facts: Ethylene. Fact sheet  

SciTech Connect

EPA is directed by the Federal Insecticide, Fungicide, and Rodenticide Act as amended in 1988 (FIFRA '88) to review all pesticide products containing active ingredients initially registered before November 1, 1984, and to reregister those products that have a substantially complete data base and do not pose unreasonable adverse effects to people or the environment. The pesticide reregistration program is to be completed by the late 1990's. The RED FACTS fact sheet summarizes EPA's conclusion, as set forth in the Reregistration Eligibility Document (or RED), that products containing a pesticide do not pose unreasonable risks when used as directed by Agency-approved labeling, and are eligible for reregistration.

Not Available

1992-09-01

283

76 FR 22033 - Safety Zone; Red River Safety Zone, Red River, MN  

Federal Register 2010, 2011, 2012, 2013, 2014

...USCG-2011-0263] RIN 1625-AAOO Safety Zone; Red River Safety Zone, Red River, MN AGENCY: Coast Guard, DHS. ACTION: Temporary...MN is establishing a temporary safety zone on the Red River, MN. This safety zone is being...

2011-04-20

284

Graft-vs-host-disease-associated conjunctival chemosis and central serous chorioretinopathy after bone marrow transplant  

Microsoft Academic Search

PURPOSE: To describe bilateral conjunctival chemosis and central serous chorioretinopathy in a patient with graft-vs-host disease after bone marrow transplant.DESIGN: Interventional case report.METHODS: A 45-year-old Chinese woman developed blurring of vision 16 days after bone marrow transplant for multiple myeloma. She had graft-vs-host disease 11 days after bone marrow transplantation. On examination, vision was 0.6 in the right eye and

Lulu L Cheng; Alvin K. H Kwok; Nelson M. S Wat; E. L Neoh; Hayden C. K Jon; Dennis S. C Lam

2002-01-01

285

A case of synovial sarcoma with bone metastasis identified by bone marrow scintigraphy  

SciTech Connect

In a patient with synovial sarcoma, routine bone survey showed no abnormality, while bone marrow scintigraphy with Tc-99m sulfur colloid revealed a defect in the fifth lumbar vertebra. At surgery, tumorous invasion was noted in the fifth lumbar vertebra and the surrounding tissues. It was suggested that the bone marrow scintigraphy was particularly useful in the detection of tumorous invasion into the bone marrow at the early stage before the destruction of skeletal tissue.

Otsuka, N.; Morita, R.; Yamamoto, T.; Muranaka, A.; Tomomitsu, T.; Yanagimoto, S.; Sone, T.; Fukunaga, M.

1985-04-01

286

Role of immobilization of irradiated rats in the protective effect of bone marrow shielding  

NASA Technical Reports Server (NTRS)

Rats were exposed to X-radiation to study the influence of immobilization and shielding of part of bone marrow during exposure on survival. It is concluded that (1) the beneficial effect of the stress factor (created by the immobilization of rats during exposure) can aggregate with the effect of bone marrow shielding and, under certain conditions, imitate the latter; and (2) the probability of the protective effect of immobilization should be taken into account when assessing the influence of bone marrow shielding.

Gronskaya, N. F.; Strelin, G. S.

1982-01-01

287

Annexin A1 regulates neutrophil clearance by macrophages in the mouse bone marrow  

PubMed Central

Under homeostatic conditions, a proportion of senescent CXCR4hi neutrophils home from the circulation back to the bone marrow, where they are phagocytosed by bone marrow macrophages. In this study, we have identified an unexpected role for the anti-inflammatory molecule annexin A1 (AnxA1) as a critical regulator of this process. We first observed that AnxA1?/? mice have significantly increased neutrophil numbers in their bone marrow while having normal levels of GM and G colony-forming units, monocytes, and macrophages. Although AnxA1?/? mice have more neutrophils in the bone marrow, a greater proportion of these cells are senescent, as determined by their higher levels of CXCR4 expression and annexin V binding. Consequently, bone marrow neutrophils from AnxA1?/? mice exhibit a reduced migratory capacity in vitro. Studies conducted in vitro also show that expression of AnxA1 is required for bone marrow macrophages, but not peritoneal macrophages, to phagocytose apoptotic neutrophils. Moreover, in vivo experiments indicate a defect in clearance of wild-type neutrophils in the bone marrow of AnxA1?/? mice. Thus, we conclude that expression of AnxA1 by resident macrophages is a critical determinant for neutrophil clearance in the bone marrow.— Dalli, J., Jones, C. P., Cavalcanti, D. M., Farsky, S. H., Perretti, M., Rankin, S. M. Annexin A1 regulates neutrophil clearance by macrophages in the mouse bone marrow. PMID:21957127

Dalli, Jesmond; Jones, Carla P.; Cavalcanti, Danielle M.; Farsky, Sandra H.; Perretti, Mauro; Rankin, Sara M.

2012-01-01

288

Effect of nephrotoxic drugs on the development of radiation nephropathy after bone marrow transplantation  

SciTech Connect

Chronic renal failure is a significant cause of late morbidity in bone marrow transplant patients whose conditioning regimen includes total body irradiation (TBI). Radiation is a major cause of this syndrome (bone marrow transplant nephropathy), but it may not be the only cause. These studies use a rat syngeneic bone marrow transplant model to determine whether nephrotoxic agents used in conjunction with bone marrow transplantation (BMT) could be enhancing or accelerating the development of radiation nephropathy. Rats received 11-17 Gy TBI in six fractions over 3 days followed by syngeneic bone marrow transplant. In conjunction with the bone marrow transplants, animals received either no drugs, cyclosporine, amphotericin, gentamicin, or busulfan. Drugs were given in schedules analogous to their use in clinical bone marrow transplantation. Drug doses were chosen so that the drug regimen alone caused detectable acute nephrotoxicity. Animals were followed for 6 months with periodic renal function tests. Gentamicin had no apparent interactions with TBI. Amphotericin increased the incidence of engraftment failure, but did not enhance radiation nephropathy. Cyclosporin with TBI caused late morbidity that appeared to be due to neurological problems, but did not enhance radiation nephropathy. Busulfan resulted in a significant enhancement of radiation nephropathy. Of the nephrotoxins used in conjunction with bone marrow transplantation only radiation and busulfan were found to be risk factors for bone marrow transplant nephropathy. 34 refs., 4 figs., 2 tabs.

Lawton, C.A.; Fish, B.L.; Moulder, J.E. (Medical College of Wisconsin, Milwaukee, WI (United States))

1994-03-01

289

Intravenous transplantation of bone marrow mesenchymal stem cells promotes neural regeneration after traumatic brain injury  

PubMed Central

To investigate the supplement of lost nerve cells in rats with traumatic brain injury by intravenous administration of allogenic bone marrow mesenchymal stem cells, this study established a Wistar rat model of traumatic brain injury by weight drop impact acceleration method and administered 3 × 106 rat bone marrow mesenchymal stem cells via the lateral tail vein. At 14 days after cell transplantation, bone marrow mesenchymal stem cells differentiated into neurons and astrocytes in injured rat cerebral cortex and rat neurological function was improved significantly. These findings suggest that intravenously administered bone marrow mesenchymal stem cells can promote nerve cell regeneration in injured cerebral cortex, which supplement the lost nerve cells. PMID:25206912

Anbari, Fatemeh; Khalili, Mohammad Ali; Bahrami, Ahmad Reza; Khoradmehr, Arezoo; Sadeghian, Fatemeh; Fesahat, Farzaneh; Nabi, Ali

2014-01-01

290

[Radiosensitivity of marrow stromal cells and the effect of some radioprotective agents].  

PubMed

The results showed that marrow stromal cells include fibroblasts, reticular cells, macrophages and adipocytes. The capability of the adherent layer derived from marrow cells of 2 mouse femurs to support hematopoietic stem cells was stronger than those of layers derived from 0.5 or 1 mouse femurs. The radiosensitivity of bone marrow stromal cells was lower than that of hematopoietic stem cells. The radioprotective effect of AET and PLP (polysaccharide of Lobaria pulmonaria Hoffm) on the bone marrow stromal cells and their capability to support hematopoietic stem cells was clearly demonstrated. PMID:1839252

Liu, S

1991-10-01

291

Induction of allogeneic unresponsiveness by supralethal irradiation and bone marrow reconstitution. [Dogs  

SciTech Connect

Supralethally irradiated dogs were reconstituted wth their own stored bone marrow and were challenged at various time intervals with a kidney allograft. The data suggest that transplanted bone marrow cells may participate directly in the events leading to allogenic unresponsiveness. The time interval between marrow cell replacement and kidney allotransplantation required for optimal results suggest that at least one cycle of cell turnover by the replaced stem cells is needed in order to produce unresponsiveness. Host irradiation and reconstitution with stored autologous marrow may be useful in the treatment of certain forms of cancer.

Rapaport, F.T.; Bachvaroff, R.J.; Akiyama, N.; Sato, T.

1980-09-01

292

Pesa Large Red Dry Bean  

Technology Transfer Automated Retrieval System (TEKTRAN)

Released in 2006, Pesa was derived from the single cross Rojo x Kablanketi made in Dec-Jan 1992-93. The parent ‘Rojo’ is a large red-seeded cultivar released by SUA in 1997. It has I bc-12 resistance to BCMV and BCMNV, resistance to the prevalent races of ALS, and moderate resistance to CBB, and H...

293

Red Means Go Structural Engineering  

E-print Network

, Founder of Acer, Taiwan, 1992 #12;Red Means Go The Horizon Report 2008 The New Media Consortium for Information Access and Malleability · All time access to all your services Phone browsing is just Generation has grown up with technology, it's use and on-demand access to information. There is evidence

294

Chili with Red Beans Ingredients  

E-print Network

Chili with Red Beans Ingredients: 1/2 pound ground beef, extra lean 1 medium onion, chopped 4 cups. 3. While beef is cooking, cut the ends off of the onion, and peel off the brown layers. Cut the onion in half lengthwise, and place the flat side on the cutting board. Slice across the onion, from one

Liskiewicz, Maciej

295

Sunset over Red Rock Canyon  

USGS Multimedia Gallery

Pine Creek Canyon is a remnant ecosystem of loblolly pines. A remnant ecosystem is the last vestige of an ecosystem type that used to be more widespred. Red Rock Canyon is a National Conservation Area managed by the Bureau of Land Management, located just outside of Las Vegas, Nevada. It is part of...

296

Growth declines in red spruce  

SciTech Connect

Over the past two decades second-growth red spruce stands in the Northeast have demonstrated declines in radial increment. Some observers are implicating air pollution as a primary cause of the declines, based on recently acquired increment cores from dominant trees. Various forms of air pollution (O/sub 3/, NO/sub x/, SO/sub 2/, and trace metals) are known to reduce growth and development of tree species, but few studies have provided concrete evidence of regional pollution-caused declines in forest ecosystems. Recently published evidence of a synchronous, consistent, and unprecedented regional decline in red spruce should be weighed against the realization that radial increment in red spruce declines naturally as stands age. Separating anthropogenic stress-caused growth patterns from natural stand dynamics requires an in-depth knowledge of forest growth and yield, tree silvics, and forest ecosystem processes. Detailed analyses of growth by stand characteristics - site index, density, elevation, stand history - will be necessary to implicate air pollution as a primary cause of red spruce decline.

Zedaker, S.M.; Hyink, D.M.; Smith, D.W.

1987-01-01

297

Characterization of DC-STAMP+ Cells in Human Bone Marrow  

PubMed Central

Osteoclasts (OC), specialized cells derived from monocytes, maintain skeletal homeostasis under normal conditions but degrade bone in patients with rheumatoid (RA) and psoriatic arthritis (PsA). Monocytes initially develop in the bone marrow (BM), circulate in peripheral blood, and differentiate into distinct cell types with diverse functions. Imaging studies in (RA) patients and murine arthritis models demonstrate that bone marrow edema detected on MRI is the result of enhanced myelopoiesis which precedes the development of bone erosions detected on plain radiographs several years later. A major knowledge gap, however, is whether OC develop in the BM and circulate to the joint and if the differentiation to OC takes place in the joint space in response to differentiation signals such as RANKL and TNF. We have previously demonstrated that osteoclast precursors (OCP) are increased in the circulaton of patients with RA and PsA. We showed that DC-STAMP (Dendritic Cell-Specific Transmembrane protein), a 7-pass transmembrane protein expressed on the surface of monocytes, is essential for cell-to-cell fusion during OC differentiation and is a valid biomarker of OCP. Herein, we examined OCP in human bone marrow and identified one novel subset of DC-STAMP+CD45intermediate monocytes which was absent in the blood. We also found that OCPs reside in human BM with a higher frequency than in the peripheral blood. These findings support the notion that the BM is a major reservoir of circulating OCPs. In addition, we demonstrated that a higher frequency of DC-STAMP+ cells in the BM have detectable intracellular IFN-?, IL-4 and IL-17A than DC-STAMP+ cells circulating in the peripheral blood. Finally, the frequency of DC-STAMP+ monocytes and T cells is signficantly higher in PsA BM compared to healthy controls, suggesting an enhanced myelopoiesis is a central event in inflammatory arthritis. PMID:25419541

Chiu, Yahui Grace; Ritchlin, Christopher T

2014-01-01

298

Red blood cells, sickle cell (image)  

MedlinePLUS

... is an inherited blood disease in which the red blood cells produce abnormal pigment (hemoglobin). The abnormal hemoglobin causes deformity of the red blood cells into crescent or sickle-shapes, as ...

299

Red in the Face: Understanding Rosacea  

MedlinePLUS

... External link, please review our exit disclaimer . Subscribe Red in the Face Understanding Rosacea Some people think ... complexion as a sign of good health. But red patches on the face may point to something ...

300

Red Grooms: Selections from the Graphic Work.  

ERIC Educational Resources Information Center

Provides background information on the life and career of Charles Rogers Grooms, better known as Red Grooms. Describes the art exhibit, "Red Grooms: Selections from the Graphic Work," presented by the Tennessee State Museum (Nashville, Tennessee). (CMK)

Johnson, Mark M.

2002-01-01

301

Avoiding Anemia: Boost Your Red Blood Cells  

MedlinePLUS

... our exit disclaimer . Subscribe Avoiding Anemia Boost Your Red Blood Cells If you’re feeling constantly exhausted ... when your body doesn’t have enough healthy red blood cells. You may either have too few ...

302

The Brain-Bone Marrow Connection: Dysfunction in Diabetes  

Cancer.gov

Despite decades of investigation that have implicated endothelial dysfunction, inflammation, and oxidative stress in diabetes-induced vascular pathophysiology, the precise sequence of cellular and molecular events that initiate and establish vascular disease in individuals with diabetes remains poorly defined. Dysfunctional brain-bone marrow communication may play a key role in this pathophysiology and promote microvascular complications. Stressors such as hyperglycemia increase the expression of the chemokine (C-C motif) ligand 2 (CCL2) and microglia activation in the paraventricular nucleus of the hypothalamus (PVN).

303

Mouse Models in Bone Marrow Transplantation and Adoptive Cellular Therapy  

PubMed Central

Mouse models of transplantation have been indispensable to the development of bone marrow transplantation (BMT). Their role in the generation of basic science knowledge is invaluable and is subject to discussion below. However, this article focuses on the direct role and relevance of mouse models towards the clinical development and advances in BMT and adoptive T-cell therapy for human diseases. The authors aim to present a thoughtful perspective on the pros and cons of mouse models while noting that despite imperfections these models are obligatory for the development of science-based medicine. PMID:24216170

Arber, Caroline; Brenner, Malcolm K.; Reddy, Pavan

2014-01-01

304

Bone marrow mononuclears from murine tibia after spaceflight on biosatellite  

NASA Astrophysics Data System (ADS)

Elucidation of the space flight effects on the adult stem and progenitor cells is an important goal in space biology and medicine. A unique opportunity for this is provided by project "BION -M1". The purpose of this study was to evaluate the effects of a 30-day flight on biosatellite "BION - M1" and the subsequent 7-day recovery on the quantity, viability, immunophenotype of mononuclears from murine tibia bone marrow. Also the in vitro characterization of functional capacity of multipotent mesenchymal stromal cells (MSCs) was scheduled. Under the project, the S57black/6 mice were divided into groups: spaceflight/vivarium control, recovery after spaceflight/ vivarium control to recovery. Bone marrow mononuclears were isolated from the tibia and immunophenotyped using antibodies against CD45, CD34, CD90 on a flow cytometer Epics XL (Beckman Coulter). A part of the each pool was frozen for subsequent estimation of hematopoietic colony-forming units (CFU), the rest was used for the evaluation of fibroblast CFU (CFUf) number, MSC proliferative activity and osteogenic potency. The cell number in the flight group was significantly lower than in the vivarium control group. There were no differences in this parameter between flight and control groups after 7 days of recovery. The mononuclears viability was more than 95 percent in all examined groups. Flow cytometric analysis showed no differences in the bone marrow cell immunophenotype (CD45, CD34, CD90.1 (Thy1)), but the flight animals had more large-sized CD45+mononuclears, than the control groups of mice. There was no difference in the CFUf number between groups. After 7 days in vitro the MSC number in flight group was twice higher than in vivarium group, after 10 days - 4 times higher. These data may indicate a higher proliferative activity of MSCs after spaceflight. MSCs showed the same and high alkaline phosphatase activity, both in flight and in the control groups, suggesting no effect of spaceflight factors on early osteogenic potency of stromal cells. These results indicate that spaceflight factors had no significant damaging effects on the murine bone marrow mononuclears. These observations are consistent with previously made assumption of moderate and reversible stress reaction of mammals on spaceflight conditions. This work was supported by Program of Basic Research of IMBP RAS

Andreeva, Elena; Roe, Maria; Buravkova, Ludmila; Andrianova, Irina; Goncharova, Elena; Gornostaeva, Alexandra

305

Bone marrow-derived CD13+ cells sustain tumor progression  

PubMed Central

Non-malignant cells found within neoplastic lesions express alanyl (membrane) aminopeptidase (ANPEP, best known as CD13), and CD13-null mice exhibit limited tumor growth and angiogenesis. We have recently demonstrated that a subset of bone marrow-derived CD11b+CD13+ myeloid cells accumulate within neoplastic lesions in several murine models of transplantable cancer to promote angiogenesis. If these findings were confirmed in clinical settings, CD11b+CD13+ myeloid cells could become a non-malignant target for the development of novel anticancer regimens. PMID:25339996

Dondossola, Eleonora; Corti, Angelo; Sidman, Richard L; Arap, Wadih; Pasqualini, Renata

2014-01-01

306

Bone marrow MR imaging findings in disuse osteoporosis  

Microsoft Academic Search

Objective  To demonstrate MR imaging findings in the cortical and trabecular bone as well as marrow changes in patients with disuse osteoporosis\\u000a (DO).\\u000a \\u000a \\u000a \\u000a \\u000a Materials and Methods  Sixteen patients (14 men, 2 women, aged 27–86 years) with clinical and radiographic evidence of DO of a lower limb joint (10\\u000a knees, 6 ankles) with MR examination of the same joint performed within a 1-month period

Marcelo R. de Abreu; Michelle Wesselly; Christine B. Chung; Donald Resnick

2011-01-01

307

Photoconversion in orange and red fluorescent proteins.  

PubMed

We found that photoconversion is fairly common among orange and red fluorescent proteins, as in a screen of 12 proteins, 8 exhibited photoconversion. Specifically, three red fluorescent proteins could be switched to a green state, and two orange variants could be photoconverted to a far-red state. The orange proteins are ideal for dual-probe highlighter applications, and they exhibited the most red-shifted excitation of all fluorescent proteins described to date. PMID:19363494

Kremers, Gert-Jan; Hazelwood, Kristin L; Murphy, Christopher S; Davidson, Michael W; Piston, David W

2009-05-01

308

Red cell distribution width and nonalcoholic steatohepatitis  

PubMed Central

Red cell distribution width is a measure of deviation of the volume of red blood cells. It is a marker of anisocytosis and often used to evaluate the possible causes of anemia. Elevated red cell distribution width levels are also associated with acute and chronic inflammatory responses. In nonalcoholic steatohepatitis, inflammation is accompanied with steatosis. For assuming red cell distribution width as a marker of nonalcoholic steatohepatitis, intervening factors such as levels of inflammatory markers should also be evaluated. PMID:25473202

Gulcan Kurt, Yasemin; Cayci, Tuncer; Aydin, Fevzi Nuri; Agilli, Mehmet

2014-01-01

309

GATA2 regulates differentiation of bone marrow-derived mesenchymal stem cells  

PubMed Central

The bone marrow microenvironment comprises multiple cell niches derived from bone marrow mesenchymal stem cells. However, the molecular mechanism of bone marrow mesenchymal stem cell differentiation is poorly understood. The transcription factor GATA2 is indispensable for hematopoietic stem cell function as well as other hematopoietic lineages, suggesting that it may maintain bone marrow mesenchymal stem cells in an immature state and also contribute to their differentiation. To explore this possibility, we established bone marrow mesenchymal stem cells from GATA2 conditional knockout mice. Differentiation of GATA2-deficient bone marrow mesenchymal stem cells into adipocytes induced accelerated oil-drop formation. Further, GATA2 loss- and gain-of-function analyses based on human bone marrow mesenchymal stem cells confirmed that decreased and increased GATA2 expression accelerated and suppressed bone marrow mesenchymal stem cell differentiation to adipocytes, respectively. Microarray analysis of GATA2 knockdowned human bone marrow mesenchymal stem cells revealed that 90 and 189 genes were upregulated or downregulated by a factor of 2, respectively. Moreover, gene ontology analysis revealed significant enrichment of genes involved in cell cycle regulation, and the number of G1/G0 cells increased after GATA2 knockdown. Concomitantly, cell proliferation was decreased by GATA2 knockdown. When GATA2 knockdowned bone marrow mesenchymal stem cells as well as adipocytes were cocultured with CD34-positive cells, hematopoietic stem cell frequency and colony formation decreased. We confirmed the existence of pathological signals that decrease and increase hematopoietic cell and adipocyte numbers, respectively, characteristic of aplastic anemia, and that suppress GATA2 expression in hematopoietic stem cells and bone marrow mesenchymal stem cells. PMID:25150255

Kamata, Mayumi; Okitsu, Yoko; Fujiwara, Tohru; Kanehira, Masahiko; Nakajima, Shinji; Takahashi, Taro; Inoue, Ai; Fukuhara, Noriko; Onishi, Yasushi; Ishizawa, Kenichi; Shimizu, Ritsuko; Yamamoto, Masayuki; Harigae, Hideo

2014-01-01

310

Histological Analyses Demonstrate the Temporary Contribution of Yolk Sac, Liver, and Bone Marrow to Hematopoiesis during Chicken Development  

PubMed Central

The use of avian animal models has contributed to the understanding of many aspects of the ontogeny of the hematopoietic system in vertebrates. However, specific events that occur in the model itself are still unclear. There is a lack of consensus, among previous studies, about which is the intermediate site responsible for expansion and differentiation of hematopoietic cells, and the liver's contribution to the development of this system. Here we aimed to evaluate the presence of hematopoiesis in the yolk sac and liver in chickens, from the stages of intra-aortic clusters in the aorta-genital ridges-mesonephros (AGM) region until hatching, and how it relates to the establishment of the bone marrow. Gallus gallus domesticus L. embryos and their respective yolk sacs at embryonic day 3 (E3) and up to E21 were collected and processed according to standard histological techniques for paraffin embedding. The slides were stained with hematoxylin-eosin, Lennert's Giemsa, and Sirius Red at pH 10.2, and investigated by light microscopy. This study demonstrated that the yolk sac was a unique hematopoietic site between E4 and E12. Hematopoiesis occurred in the yolk sac and bone marrow between E13 and E20. The liver showed granulocytic differentiation in the connective tissue of portal spaces at E15 and onwards. The yolk sac showed expansion of erythrocytic and granulocytic lineages from E6 to E19, and E7 to E20, respectively. The results suggest that the yolk sac is the major intermediate erythropoietic and granulopoietic site where expansion and differentiation occur during chicken development. The hepatic hematopoiesis is restricted to the portal spaces and represented by the granulocytic lineage. PMID:24621665

Guedes, Priscila Tavares; de Oliveira, Barbara Cristina Euzébio Pereira Dias; Manso, Pedro Paulo de Abreu; Caputo, Luzia Fátima Gonçalves; Pelajo-Machado, Marcelo

2014-01-01

311

Increased expression of TIGIT on CD4+ T cells ameliorates immune-mediated bone marrow failure of aplastic anemia.  

PubMed

Aplastic anemia (AA) is an autoimmune disease in which T cell activation is suspected to play an important role. T cell immunoglobulin and ITIM (immunoreceptor tyrosine-based inhibition motif) domain (TIGIT) is an inhibitory receptor, which exhibits inhibitory functions on the immune response. However, its role in AA has not been clearly determined. In the current study, we showed that the frequency of TIGIT-positive CD4(+) T cells was reduced in the vast majority of AA patients (85%, 17/20). In TIGIT-silenced human CD4(+) T cells, stimulation of agonistic anti-TIGIT monoclonal antibody significantly facilitated cell proliferation, increased production of IL-2 and IFN-?, and inhibited production of IL-10. However, in TIGIT-overexpressed human CD4(+) T cells, cell proliferation and the production of IL-2, IFN-?, and TNF-? were significantly hindered; in contrast, the secretion of IL-10 was improved. RT-PCR and Western blotting showed that T-bet expression in human CD4(+) T cells was significantly decreased by TIGIT overexpression, but only slightly altered by TIGIT knockdown. In mouse models, lentivirus-mediated TIGIT-overexpressed CD4(+) T cell transfer significantly rescued the decreased red blood cell count, attenuated the increase in serum INF-? and TNF-? levels, and lengthened the median survival time. The mRNA levels of CD34, stem cell factor (SCF), and granulocyte/macrophage-colony-stimulating factor (GM-CSF) in bone marrow mononuclear cells were also up-regulated. In conclusion, increased expression of TIGIT could inhibit the function of CD4(+) T cells in vitro and ameliorate immune-mediated bone marrow failure of AA in vivo providing a new potential strategy for the treatment of AA. PMID:24905442

Zhang, Tao; Wang, Jianhong; Zhou, Xingchun; Liang, Rong; Bai, Qingxian; Yang, Lan; Gu, Hongtao; Gao, Guangxun; Dong, Baoxia; Zhu, Huafeng; Chen, Xiequn

2014-11-01

312

Enhanced green fluorescent protein is a nearly ideal long-term expression tracer for hematopoietic stem cells, whereas DsRed-express fluorescent protein is not.  

PubMed

Validated gene transfer and expression tracers are essential for elucidating functions of mammalian genes. Here, we have determined the suitability and unintended side effects of enhanced green fluorescent protein (EGFP) and DsRed-Express fluorescent protein as expression tracers in long-term hematopoietic stem cells (HSCs). Retrovirally transduced mouse bone marrow cells expressing either EGFP or DsRed-Express in single or mixed dual-color cell populations were clearly discerned by flow cytometry and fluorescence microscopy. The results from in vivo competitive repopulation assays demonstrated that EGFP-expressing HSCs were maintained nearly throughout the lifespan of the transplanted mice and retained long-term multilineage repopulating potential. All mice assessed at 15 months post-transplantation were EGFP positive, and, on average, 24% total peripheral white blood cells expressed EGFP. Most EGFP-expressing recipient mice lived at least 22 months. In contrast, Discosoma sp. red fluorescent protein (DsRed)-expressing donor cells dramatically declined in transplant-recipient mice over time, particularly in the competitive setting, in which mixed EGFP- and DsRed-expressing cells were cotransplanted. Moreover, under in vitro culture condition favoring preservation of HSCs, purified EGFP-expressing cells grew robustly, whereas DsRed-expressing cells did not. Therefore, EGFP has no detectable deteriorative effects on HSCs, and is nearly an ideal long-term expression tracer for hematopoietic cells; however, DsRed-Express fluorescent protein is not suitable for these cells. PMID:17138958

Tao, Wen; Evans, Barbara-Graham; Yao, Jing; Cooper, Scott; Cornetta, Kenneth; Ballas, Christopher B; Hangoc, Giao; Broxmeyer, Hal E

2007-03-01

313

COUNTS OF RED TIDE ORGANISMS Gymnodinium breve  

E-print Network

COUNTS OF RED TIDE ORGANISMS Gymnodinium breve AND ASSOCIATED OCEANOGRAPHIC DATA FROM FLORIDA WEST, Fred A. Seaton, Secretary Fish and Wildlife Service, Arnie J. Suoraela, Commissioner COUNTS OF RED TIDE of red tide organisms, Gymnodinium breve, and associated oceanographic data from Florida west coast, 1954

314

NOAA New England Red Tide Information Center  

NSDL National Science Digital Library

This NOAA website offers a brief explanation of red tide and a large bloom that spread from the Gulf of Maine to the Massachusetts Bay in 2005. The site explains NOAA's efforts to remediate red tide in New England as well as prevent new occurrences. The site features hyperlinks to research funding, legislation, and other NOAA sites that feature red tide.

315

7 CFR 29.1053 - Red (R).  

Code of Federal Regulations, 2010 CFR

...Agriculture 2 2010-01-01 2010-01-01 false Red (R). 29.1053 Section 29.1053 Agriculture Regulations...Types 11, 12, 13, 14 and Foreign Type 92) § 29.1053 Red (R). A brownish red. [42 FR 21092, Apr. 25, 1977....

2010-01-01

316

Combating red tape in the public sector  

Microsoft Academic Search

It seems that the phenomenon of red tape may best be understood as a problem of individual perceptions rather than one of identifying objective measures for it. The result of these individual perceptions is that one person?s red tape may be another?s sacred protection. In a quest to obtain a better understanding of red tape it has been linked, for

Dirk J. Brynard

1995-01-01

317

27 CFR 9.167 - Red Mountain  

Code of Federal Regulations, 2013 CFR

...2013-04-01 2013-04-01 false Red Mountain 9.167 Section 9.167 ...American Viticultural Areas § 9.167 Red Mountain (a) Name. The name of the...viticultural area described in this section is “Red Mountain.” (b) Approved maps....

2013-04-01

318

21 CFR 184.1121 - Red algae.  

Code of Federal Regulations, 2013 CFR

...Drugs 3 2013-04-01 2013-04-01 false Red algae. 184.1121 Section 184.1121 Food and...Specific Substances Affirmed as GRAS § 184.1121 Red algae. (a) Red algae are seaweeds of the species Gloiopeltis...

2013-04-01

319

27 CFR 9.167 - Red Mountain  

Code of Federal Regulations, 2012 CFR

...2012-04-01 2012-04-01 false Red Mountain 9.167 Section 9.167 ...American Viticultural Areas § 9.167 Red Mountain (a) Name. The name of the...viticultural area described in this section is “Red Mountain.” (b) Approved maps....

2012-04-01

320

27 CFR 9.167 - Red Mountain  

Code of Federal Regulations, 2011 CFR

...2011-04-01 2011-04-01 false Red Mountain 9.167 Section 9.167 ...American Viticultural Areas § 9.167 Red Mountain (a) Name. The name of the...viticultural area described in this section is “Red Mountain.” (b) Approved maps....

2011-04-01

321

27 CFR 9.167 - Red Mountain  

Code of Federal Regulations, 2014 CFR

...2014-04-01 2014-04-01 false Red Mountain 9.167 Section 9.167 ...American Viticultural Areas § 9.167 Red Mountain (a) Name. The name of the...viticultural area described in this section is “Red Mountain.” (b) Approved maps....

2014-04-01

322

21 CFR 184.1121 - Red algae.  

Code of Federal Regulations, 2014 CFR

...Drugs 3 2014-04-01 2014-04-01 false Red algae. 184.1121 Section 184.1121 Food and...Specific Substances Affirmed as GRAS § 184.1121 Red algae. (a) Red algae are seaweeds of the species Gloiopeltis...

2014-04-01

323

27 CFR 9.167 - Red Mountain  

Code of Federal Regulations, 2010 CFR

...2010-04-01 2010-04-01 false Red Mountain 9.167 Section 9.167 ...American Viticultural Areas § 9.167 Red Mountain (a) Name. The name of the...viticultural area described in this section is “Red Mountain.” (b) Approved maps....

2010-04-01

324

Code red worm propagation modeling and analysis  

Microsoft Academic Search

The Code Red worm incident of July 2001 has stimulated activities to model and analyze Internet worm propagation. In this paper we provide a careful analysis of Code Red propagation by accounting for two factors: one is the dynamic countermeasures taken by ISPs and users; the other is the slowed down worm infection rate because Code Red rampant propagation caused

Cliff Changchun Zou; Weibo Gong; Donald F. Towsley

2002-01-01

325

21 CFR 184.1121 - Red algae.  

Code of Federal Regulations, 2011 CFR

...Drugs 3 2011-04-01 2011-04-01 false Red algae. 184.1121 Section 184.1121 Food and...Specific Substances Affirmed as GRAS § 184.1121 Red algae. (a) Red algae are seaweeds of the species Gloiopeltis...

2011-04-01

326

21 CFR 184.1121 - Red algae.  

Code of Federal Regulations, 2010 CFR

...Drugs 3 2010-04-01 2009-04-01 true Red algae. 184.1121 Section 184.1121 Food and...Specific Substances Affirmed as GRAS § 184.1121 Red algae. (a) Red algae are seaweeds of the species Gloiopeltis...

2010-04-01

327

76 FR 23485 - Safety Zone; Red River  

Federal Register 2010, 2011, 2012, 2013, 2014

...USCG-2011-0260] RIN 1625-AA00 Safety Zone; Red River AGENCY: Coast Guard, DHS. ACTION...temporary safety zone for all waters of the Red River in the State of North Dakota, including...associated with flooding occurring on the Red River. Entry into this zone is...

2011-04-27

328

39 CFR 259.2 - Red Cross.  

Code of Federal Regulations, 2011 CFR

... 2011-07-01 2011-07-01 false Red Cross. 259.2 Section 259.2 Postal...SERVICES PERFORMED FOR OTHER AGENCIES § 259.2 Red Cross. (a) General. The Postal Service and the Red Cross cooperate to maintain...

2011-07-01

329

39 CFR 259.2 - Red Cross.  

Code of Federal Regulations, 2010 CFR

... 2010-07-01 2010-07-01 false Red Cross. 259.2 Section 259.2 Postal...SERVICES PERFORMED FOR OTHER AGENCIES § 259.2 Red Cross. (a) General. The Postal Service and the Red Cross cooperate to maintain...

2010-07-01

330

39 CFR 259.2 - Red Cross.  

Code of Federal Regulations, 2013 CFR

... 2013-07-01 2013-07-01 false Red Cross. 259.2 Section 259.2 Postal...SERVICES PERFORMED FOR OTHER AGENCIES § 259.2 Red Cross. (a) General. The Postal Service and the Red Cross cooperate to maintain...

2013-07-01

331

21 CFR 184.1121 - Red algae.  

Code of Federal Regulations, 2012 CFR

...Drugs 3 2012-04-01 2012-04-01 false Red algae. 184.1121 Section 184.1121 Food and...Specific Substances Affirmed as GRAS § 184.1121 Red algae. (a) Red algae are seaweeds of the species Gloiopeltis...

2012-04-01

332

39 CFR 259.2 - Red Cross.  

Code of Federal Regulations, 2014 CFR

... 2014-07-01 2014-07-01 false Red Cross. 259.2 Section 259.2 Postal...SERVICES PERFORMED FOR OTHER AGENCIES § 259.2 Red Cross. (a) General. The Postal Service and the Red Cross cooperate to maintain...

2014-07-01

333

39 CFR 259.2 - Red Cross.  

Code of Federal Regulations, 2012 CFR

... 2012-07-01 2012-07-01 false Red Cross. 259.2 Section 259.2 Postal...SERVICES PERFORMED FOR OTHER AGENCIES § 259.2 Red Cross. (a) General. The Postal Service and the Red Cross cooperate to maintain...

2012-07-01

334

Scottish Strategy for Red Squirrel Conservation  

E-print Network

#12;#12;Scottish Strategy for Red Squirrel Conservation 2004 Edited and Produced by the Scottish the co-ordination of activities aiming to conserve the red squirrel in Scotland. It is co-statutory organisations, together with representatives of local voluntary squirrel groups dedicated to conserving red

335

Italian Herb Red Potato Salad Ingredients  

E-print Network

. Carefully stir in bell peppers and onions. 6. Cover and chill until ready to serve. Equipment: Cutting boardItalian Herb Red Potato Salad Ingredients: 1 1/2 pounds red potatoes 1 green pepper 1 red onion 1/4 teaspoon garlic powder 1/4 teaspoon ground black pepper Directions 1. Wash potatoes, and cut into quarters

Liskiewicz, Maciej

336

Red Hat and Condor Project Matthew Farrellee  

E-print Network

Red Hat and Condor Project Matthew Farrellee Principal Software Engineer #12;Today... History MRG status Open source Management Cloud #12;Condor Week 2007 -Red Hat/Condor collaboration *Condor open source *Red Hat Madison office Condor 7.0 Release -1st release with source -Condor into Fedora -Beta MRG

Liblit, Ben

337

Modulation of osteoclastogenesis in porcine bone marrow cultures by quercetin and rutin.  

PubMed

Flavonols, in contrast to soybean isoflavones, are the most abundant phytoestrogens in western diets, being present in onions, beans, fruits, red wine, and tea. They may protect against atherosclerosis, inhibit certain cancer cell types, and reduce bone resorption. The most widely distributed flavonol is quercetin, which occurs mainly as its glycoside, rutin, but data are very scarce regarding the precise mechanism of action of these compounds on bone-resorbing cells at concentrations similar to those detected in human plasma. We have therefore investigated the effects of nanomolar concentrations of quercetin and rutin on the development and activity of osteoclasts in vitro compared with the effects of 17beta-estradiol. Nonadherent porcine bone marrow cells were cultured on dentine slices in the presence of 10 nM 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), with or without 10 nM quercetin, 10 nM rutin or 10 nM 17beta-estradiol for 11 days. Multinuclear TRAP+ cells that resorbed dentine (osteoclasts) developed in the presence of 1,25(OH)2D3, but their number was significantly reduced by quercetin, rutin, and 17beta-estradiol (P < 0.05). Like 17beta-estradiol, both flavonols also significantly reduced resorption (P<0.05) as assessed by the size of pits resorbed on dentine slices. Osteoclasts and osteoclast progenitors contained estrogen receptor alpha (ERalpha), ERbeta, and RANK proteins. Both flavonols increased nuclear ERbeta protein and decreased ERalpha protein of osteoclast progenitors. Moreover, rutin reduced RANK protein, whereas 17beta-oestradiol and quercetin promoted apoptosis by cleavage of caspase-8 and caspase-3. All the effects of flavonols were reversed by 1 microM ICI 182,780, an estrogen antagonist. Thus, the anti-resorbing properties of flavonols are mainly mediated by ER proteins through the inhibition of RANK protein or the activation of caspases. PMID:15688188

Rassi, Claudia M; Lieberherr, Michele; Chaumaz, Gilles; Pointillart, Alain; Cournot, Giulia

2005-03-01

338

33 CFR 165.T09-0263 - Safety zone; Red River Safety Zone, Red River, MN.  

Code of Federal Regulations, 2011 CFR

...2011-07-01 2011-07-01 false Safety zone; Red River Safety Zone, Red River, MN. 165.T09-0263 Section 165.T09-0263...Guard District § 165.T09-0263 Safety zone; Red River Safety Zone, Red River, MN. (a)...

2011-07-01

339

RED MOUNTAIN BAR PUMPED STOR AGE PR OJEC T Red Mountain Bar Pumped Storage Project  

E-print Network

RED MOUNTAIN BAR PUMPED STOR AGE PR OJEC T Red Mountain Bar Pumped Storage Project Continuing a pumped storage project to generate electricity during peak demand. The proposed Red Mountain Bar Pumped for irrigation water storage, flood control, power production and recreation, the Red Mountain Bar Pumped Storage

Laughlin, Robert B.

340

Clifford the Big Red Dog  

NSDL National Science Digital Library

PBS Kids has companion sites for their programs that offer a number of activities for young users. This one features everyone's favorite enormous, bright-red pooch, Clifford. At Clifford's site, visitors can play a matching game, read a story, print a cut-out dog house, and print out pages to color. The site also offers some Clifford video clips. THe site is compact, easy to navigate, and well suited for their target audience.

2000-01-01

341

Why Owens Lake is Red!  

NSDL National Science Digital Library

This article explains the biological phenomena of reddish-colored salt lakes, such as Lake Owens, and playas. Applying colorful images and geological history, the author describes the processes by which astronomical numbers of microscopic, unicellular organisms living in the water and salt crust can cause the red coloration. Descriptions also include the ability of halophilic microbes to withstand high salt concentrations and other extreme conditions.

Armstrong, Wayne; Department, Palomar C.

342

Science Nation: Disappearing Red Shrimp  

NSDL National Science Digital Library

Along the shore in Hawaii, the ponds imbedded in lava rocks contain organisms found only in that environment. Among the marine life in these pools are hundreds of tiny red shrimp. With support from the National Science Foundation (NSF), molecular biologist Scott Santos and his team at Auburn University are studying how the shrimp, along with other organisms, thrive in harsh, brackish pools of water.

343

Red Scare: An Image Database  

NSDL National Science Digital Library

The William & Anita Newman Library at Baruch College (The City University of New York) has recently added this exhibit to its digital collection. Red Scare is an image database created by Leo Robert Klein, Web Coordinator and Digital Resources Developer for Newman Library. The database contains 137 photographs and political cartoons that illustrate US political history from 1918 to 1920. The images are arranged chronologically and by subject. A list of subject headings are provided.

344

The effects of simulated hypogravity on murine bone marrow cells  

NASA Technical Reports Server (NTRS)

Mouse bone marrow cells grown in complete medium at unit gravity were compared with a similar population cultured in conditions that mimic some aspects of microgravity. After the cells adjusted to the conditions that simulated microgravity, they proliferated as fetal or oncogenic populations; their numbers doubled in twelve hour periods. Differentiated subpopulations were depleted from the heterogeneous mixture with time and the undifferentiated hematopoietic stem cells increased in numbers. The cells in the control groups in unit gravity and those in the bioreactors in conditions of microgravity were monitored under a number of parameters. Each were phenotyped as to cell surface antigens using a panel of monoclonal antibodies and flow cytometry. Other parameters compared included: pH, glucose uptake, oxygen consumption and carbon-dioxide production. Nuclear DNA was monitored by flow cytometry. Functional responses were studied by mitogenic stimulation by various lectins. The importance of these findings should have relevance to the space program. Cells should behave predictably in zero gravity; specific populations can be eliminated from diverse populations and other populations isolated. The availability of stem cell populations will enhance both bone marrow and gene transplant programs. Stem cells will permit developmental biologists study the paths of hematopoiesis.

Lawless, Desales

1989-01-01

345

Bone marrow versus thymic pathways of natural killer cell development.  

PubMed

Understanding natural killer (NK) cell developmental pathways is crucial for harnessing the potential therapeutic benefits of this specialized lymphocyte subset. The bone marrow (BM) plays a major role in NK cell development, providing the appropriate environmental cues for NK cell commitment and subsequent NK cell differentiation. Nevertheless, the molecular signals provided in this context remain enigmatic. It is widely assumed that BM seeds the periphery with NK cells. However, the precise origins of NK cells found in lymphoid organs and tissues are not defined. Recently, we found that thymic NK cells bear molecular markers and functional attributes that distinguish them from most peripheral NK cells. We find that NK cells are actively exported from the thymus to the periphery, suggesting that thymus-derived NK cells may have unique roles both intrathymically and in secondary lymphoid organs. Here we compare the properties of thymic NK cells with properties of other NK cell subsets that have been identified in the mouse. We propose that heterogeneity in NK cell function can be achieved through distinct thymic and bone marrow pathways of NK cell development. PMID:17100874

Di Santo, James P; Vosshenrich, Christian A J

2006-12-01

346

[A case of inflammatory demyelinative myelopathy after bone marrow transplantation].  

PubMed

We experienced a case of demyelinating, inflammatory cervical myelopathy after bone marrow transplantation for chronic myelocytic leukemia (CML). A 28 years-old man who had been having skin and liver graft versus host disease (GVHD), developed paresthesia in the legs, and then, difficulty in walking. At the time of admission, weakness of the hands also appeared. There was no evidence of CML recurrence after bone marrow transplantation. The myelopathy was characterized by multiple abnormal spotty signal intensities in the cervical spinal cord on MRI and these were in part Gd-enhanced. A course of pulse-dose methylprednisolone was given, followed by prednisolone. The neurological deficits were improved to the degree of full recovery. The inflammatory myelopathy together with a plaque in the cerebral hemisphere, moderately delayed p-100 latency of VEP and elevation of myelin basic protein of the spinal fluid, is difficult to distinguish from that of multiple sclerosis. Although the precise mechanism of GVHD-myelopathy is not known, it is likely that the donor myelin-reactive T-lymphocytes were non-specifically activated with GVHD reaction and directed to a central nervous system. Tacrolimus might have precipitated the focal immune reaction by way of cytotoxic effects on brain capillaries. The "GVHD-myelopathy" presented here may thus be akin to multiple sclerosis in its immune mechanism. PMID:11086392

Saito, N; Tanaka, T; Matsuda, S; Inoue, M; Yamamoto, T

2000-06-01

347

Information needs of the patients undergoing bone marrow transplantation.  

PubMed

The number of allogenic and autologous bone marrow transplantation (BMT) and the number of centers which perform these procedures have increased dramatically over the last 10 years. This increase can be attributed to the progress in supportive care which caused a significant decrease of transplant related mortality. Hematopoietic stem cell transplantation (HSCT) is now established therapy for many hematological malignancies, solid tumors and autoimmune disease. In spite of the great progress made, BMT is still a drastic procedure that may make patients suffer very much and can be even fatal. Careful exploration of the social and psychological situation of the patient is a very important factor which may prevent future difficulties. Many patients suffer psychological difficulties related to bone marrow transplantation from inadequate psycho-social preparation and support. It is important to prepare an information document about the newly diagnosed hematological malignancies. It must be a clear language and must answer the initial questions the patient will ask after diagnosis is made. The information leaflet distributed to the patients suffering from hematological malignancies can play an important role in the recovery of the immune system, thereby promoting a return to health. PMID:12898837

Krasuska, Ma?gorzata E; Dmoszy?ska, Anna; Daniluk, Jadwiga; Stanis?awek, Andrzej

2002-01-01

348

Isolation and intravenous injection of murine bone marrow derived monocytes.  

PubMed

As a subtype of leukocytes and progenitors of macrophages, monocytes are involved in many important processes of organisms and are often the subject of various fields in biomedical science. The method described below is a simple and effective way to isolate murine monocytes from heterogeneous bone marrow. Bone marrow from the femur and tibia of Balb/c mice is harvested by flushing with phosphate buffered saline (PBS). Cell suspension is supplemented with macrophage-colony stimulating factor (M-CSF) and cultured on ultra-low attachment surfaces to avoid adhesion-triggered differentiation of monocytes. The properties and differentiation of monocytes are characterized at various intervals. Fluorescence activated cell sorting (FACS), with markers like CD11b, CD115, and F4/80, is used for phenotyping. At the end of cultivation, the suspension consists of 45%± 12% monocytes. By removing adhesive macrophages, the purity can be raised up to 86%± 6%. After the isolation, monocytes can be utilized in various ways, and one of the most effective and common methods for in vivo delivery is intravenous tail vein injection. This technique of isolation and application is important for mouse model studies, especially in the fields of inflammation or immunology. Monocytes can also be used therapeutically in mouse disease models. PMID:25591000

Wagner, Martin; Koester, Helen; Deffge, Christian; Weinert, Soenke; Lauf, Johannes; Francke, Alexander; Lee, Jerry; Braun-Dullaeus, R C; Herold, Joerg

2014-01-01

349

Identification of a clonally expanding haematopoietic compartment in bone marrow  

PubMed Central

In mammals, postnatal haematopoiesis occurs in the bone marrow (BM) and involves specialized microenvironments controlling haematopoietic stem cell (HSC) behaviour and, in particular, stem cell dormancy and self-renewal. While these processes have been linked to a number of different stromal cell types and signalling pathways, it is currently unclear whether BM has a homogenous architecture devoid of structural and functional partitions. Here, we show with genetic labelling techniques, high-resolution imaging and functional experiments in mice that the periphery of the adult BM cavity harbours previously unrecognized compartments with distinct properties. These units, which we have termed hemospheres, were composed of endothelial, haematopoietic and mesenchymal cells, were enriched in CD150+ CD48? putative HSCs, and enabled rapid haematopoietic cell proliferation and clonal expansion. Inducible gene targeting of the receptor tyrosine kinase VEGFR2 in endothelial cells disrupted hemospheres and, concomitantly, reduced the number of CD150+ CD48? cells. Our results identify a previously unrecognized, vessel-associated BM compartment with a specific localization and properties distinct from the marrow cavity. PMID:23188081

Wang, Lin; Benedito, Rui; Bixel, M Gabriele; Zeuschner, Dagmar; Stehling, Martin; Sävendahl, Lars; Haigh, Jody J; Snippert, Hugo; Clevers, Hans; Breier, Georg; Kiefer, Friedemann; Adams, Ralf H

2013-01-01

350

Single molecule fluorescence spectroscopy of mutants of the Discosoma red fluorescent protein DsRed  

NASA Astrophysics Data System (ADS)

We studied the emission of mutants of the red fluorescent protein DsRed by room temperature single molecule fluorescence spectroscopy. Bulk samples of the DsRed variant E8 show mixed green and red fluorescence of equivalent intensities individually spectrally similar to arrested green and mature red fluorescent forms of DsRed. Investigations at the single molecule level indicate that, like DsRed, E8 is not monomeric at single molecule concentrations. The entities visualized are composed of green and red emitting proteins without a fixed ratio of green to red fluorescing units. We find indications for only weak, if any, fluorescence resonance energy transfer (FRET) between red and green chromophores within one E8 entity.

Blum, Christian; Subramaniam, Vinod; Schleifenbaum, Frank; Stracke, Frank; Angres, Brigitte; Terskikh, Alexey; Meixner, A. J.

2002-08-01

351

Gene Therapy in Peripheral Blood Lymphocytes and Bone Marrow for ADA^- Immunodeficient Patients  

Microsoft Academic Search

Adenosine deaminase (ADA) deficiency results in severe combined immunodeficiency, the first genetic disorder treated by gene therapy. Two different retroviral vectors were used to transfer ex vivo the human ADA minigene into bone marrow cells and peripheral blood lymphocytes from two patients undergoing exogenous enzyme replacement therapy. After 2 years of treatment, long-term survival of T and B lymphocytes, marrow

Claudio Bordignon; Luigi D. Notarangelo; Nadia Nobili; Giuliana Ferrari; Giulia Casorati; Paola Panina; Evelina Mazzolari; Daniela Maggioni; Claudia Rossi; Paolo Servida; Alberto G. Ugazio; Fulvio Mavilio

1995-01-01

352

Genomic analysis of bone marrow failure and myelodysplastic syndromes reveals phenotypic and diagnostic complexity  

PubMed Central

Accurate and timely diagnosis of inherited bone marrow failure and inherited myelodysplastic syndromes is essential to guide clinical management. Distinguishing inherited from acquired bone marrow failure/myelodysplastic syndrome poses a significant clinical challenge. At present, diagnostic genetic testing for inherited bone marrow failure/myelodysplastic syndrome is performed gene-by-gene, guided by clinical and laboratory evaluation. We hypothesized that standard clinically-directed genetic testing misses patients with cryptic or atypical presentations of inherited bone marrow failure/myelodysplastic syndrome. In order to screen simultaneously for mutations of all classes in bone marrow failure/myelodysplastic syndrome genes, we developed and validated a panel of 85 genes for targeted capture and multiplexed massively parallel sequencing. In patients with clinical diagnoses of Fanconi anemia, genomic analysis resolved subtype assignment, including those of patients with inconclusive complementation test results. Eight out of 71 patients with idiopathic bone marrow failure or myelodysplastic syndrome were found to harbor damaging germline mutations in GATA2, RUNX1, DKC1, or LIG4. All 8 of these patients lacked classical clinical stigmata or laboratory findings of these syndromes and only 4 had a family history suggestive of inherited disease. These results reflect the extensive genetic heterogeneity and phenotypic complexity of bone marrow failure/myelodysplastic syndrome phenotypes. This study supports the integration of broad unbiased genetic screening into the diagnostic workup of children and young adults with bone marrow failure and myelodysplastic syndromes. PMID:25239263

Zhang, Michael Y.; Keel, Siobán B.; Walsh, Tom; Lee, Ming K.; Gulsuner, Suleyman; Watts, Amanda C.; Pritchard, Colin C.; Salipante, Stephen J.; Jeng, Michael R.; Hofmann, Inga; Williams, David A.; Fleming, Mark D.; Abkowitz, Janis L.; King, Mary-Claire; Shimamura, Akiko

2015-01-01

353

Imaging of bone infection with labelled white blood cells role of contemporaneous bone marrow imaging  

Microsoft Academic Search

The uptake of white blood cells (WBC) into normal bone marrow may lead to difficulty in detecting bone infection. Twenty-one patients in whom the WBC scan was equivocal or positive underwent a technetium 99m colloid scan to show the distribution of bone marrow. Six patients had a positive WBC scan, and in five of them a discordant colloid scan confirmed

A. D. King; A. M. Peters; A. W. J. Stuttle; J. P. Lavender

1990-01-01

354

Cigarette Smoking Causes Sequestration of Polymorphonuclear Leukocytes Released from the Bone Marrow in Lung Microvessels  

Microsoft Academic Search

Studies from our laboratory have shown that chronic cigarette smoke exposure causes a neutrophilia asso- ciated with a shortening of the mean transit time of polymorphonuclear leukocytes (PMN) though the post- mitotic pool of the marrow. The present study was designed to test the hypothesis that PMN newly re- leased from bone marrow by smoke exposure preferentially sequestered in pulmonary

Takeshi Terashima; Maria E. Klut; Dean English; Jennifer Hards; James C. Hogg; Stephan F. van Eeden

355

Repair of Infarcted Myocardium by Autologous Intracoronary Mononuclear Bone Marrow Cell Transplantation in Humans  

Microsoft Academic Search

Background—Experimental data suggest that bone marrow- derived cells may contribute to the healing of myocardial infarction (MI). For this reason, we analyzed 10 patients who were treated by intracoronary transplantation of autologous, mononuclear bone marrow cells (BMCs) in addition to standard therapy after MI. Methods and Results—After standard therapy for acute MI, 10 patients were transplanted with autologous mononuclear BMCs

Bodo E. Strauer; Michael Brehm; Tobias Zeus; Matthias Köstering; Anna Hernandez; Rudiger V. Sorg; Gesine Kögler; Peter Wernet

2009-01-01

356

Donor Leukocyte Transfusions for Treatment of Recurrent Chronic Myelogenous Leukemia in Marrow Transplant Patients  

Microsoft Academic Search

LLOGENEIC bone marrow transplantation (BMT) is A currently the only curative treatment for chronic myelogenous leukemia (CML).'-4 Among patients with hema- tologic relapse after BMT, cytogenetic remissions are rare5 unless second transplants are performed. We report here the successful treatment of hematologic relapse with interferon a (IFNa) and transfusion of viable buffy coat cells from the marrow donor without additional

H. J. Kolb; J. Mittermuller; Ch. Clemm; E. Holler; G. Ledderose; G. Brehm; M. Heim; W. Wilmanns

357

Granulocytosis-inducing tumor inhibits the production of B lymphocytes in murine bone marrow  

Microsoft Academic Search

Mice bearing a transplantable CE mammary carcinoma have been shown to have greatly augmented rates of neutrophil production coupled with a marked diminution of bone marrow lymphocytes. The objective the present study was to test whether the loss of lymphocytes, and especially of B cells, from the bone marrow and spleen of tumor-bearing animals was due to a reduced rate

G. Fueloep; M. Y. Lee; C. Rosse

1985-01-01

358

Ultrastructural changes of bone marrow in canine cyclic hematopoiesis (CH dog)  

Microsoft Academic Search

The pathogenesis of cyclic hematopoiesis (CH) in the grey collie dog is still unknown. It has been proposed that periodic bursts of necrosis of the bone marrow neutrophils would induce cyclic arrests of the stem cell differentiation. In the present study, the sequential changes undergone by the erythroid and neutrophil series of the bone marrow of CH dogs were evaluated

E. A. Machado; J. B. Jones; M. C. Aggio; A. I. Chernoff; P. A. Maxwell; R. D. Lange

1981-01-01

359

Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells in pellet cultural system  

Microsoft Academic Search

ObjectivePluripotent mesenchymal stem cells (MSC) have been isolated and well characterized from several tissue sources, including bone marrow stroma. MSC from different animals showed slight differences in morphology and in the potential to differentiate. In the present study, we isolated MSC from bovine bone marrow and induced chondrogenesis in order to establish a new experimental model of stem cell research.

Darko Bosnakovski; Morimichi Mizuno; Gonhyung Kim; Taketo Ishiguro; Masahiro Okumura; Toshihiko Iwanaga; Tsuyoshi Kadosawa; Toru Fujinaga

2004-01-01

360

Marrow transplantation from unrelated donors for patients with severe aplastic anemia who have failed immunosuppressive therapy  

Microsoft Academic Search

Allogeneic marrow transplantation offers curative therapy for patients with aplastic anemia. We analyzed retrospective results in 141 patients with severe aplastic anemia who received transplants between 1988 and 1995 from an unrelated volunteer donor identified through the National Marrow Donor Program (NMDP). All patients had failed one or more courses of immunosuppressive therapy. Of the patients, 121 (86%) received a

H. Joachim Deeg; Kristy Seidel; Jim Casper; Claudio Anasetti; Stella Davies; James L Gajewski; Mary Territo; Norma Ramsay; Richard E Harris; Hugo Castro-Malaspina; Robert Collins; Richard Champlin; Gary Schoch; Roberta King; Craig Howe

1999-01-01

361

Marrow Transplantation With or Without Donor Buffy Coat Cells for 65 Transfused Aplastic Anemia Patients  

Microsoft Academic Search

Sixty-five multiply transfused patients with severe aplastic anemia were given cyclophosphamide followed by grafts from HLA-identical siblings. The effect of the administra- tion of viable donor buffy coat cells following the marrow inoculum was evaluated with regard to graft rejection and survival. Results in 43 patients so treated are presented along with those in 22 concurrent patients given marrow alone.

Rainer Storb; Kristine C. Doney; E. D. Thomas; Fred Appelbaum; C. Dean Buckner; Reginald A. CIift; H. Joachim; Paul L. Weiden; Robert P. Witherspoon

1982-01-01

362

Moffitt research finds no survival advantage for stem cell versus bone marrow transplant  

Cancer.gov

Researchers at Moffitt Cancer Center, and colleagues in the Blood and Marrow Transplant Clinical Trials Network conducted a two-year clinical trial comparing two-year survival probabilities for patients transplanted with peripheral blood stem cells or bone marrow stem cells from unrelated donors and found no survival advantage for one method over the other.

363

Clonal Characterization of Bone Marrow Derived Stem Cells and Their Application for Bone Regeneration  

PubMed Central

Tissue engineering allows the design of functionally active cells within supportive bio-scaffolds to promote the development of new tissues such as cartilage and bone for the restoration of pathologically altered tissues. However, all bone tissue engineering applications are limited by a shortage of stem cells. The adult bone marrow stroma contains a subset of nonhematopoietic cells referred to as bone marrow mesenchymal stem cells (BMSCs). BMSCs are of interest because they are easily isolated from a small aspirate of bone marrow and readily generate single-cell-derived colonies. These cells have the capacity to undergo extensive replication in an undifferentiated state ex vivo. In addition, BMSCs have the potential to develop either in vitro or in vivo into distinct mesenchymal tissues, including bone, cartilage, fat, tendon, muscle, and marrow stroma. Thus, BMSCs are an attractive cell source for tissue engineering approaches. However, BMSCs are not homogeneous and the quantity of stem cells decreases in the bone marrow in aged population. A sequential loss of lineage differentiation potential has been found in the mixed culture of bone marrow stromal cells due to a heterogenous population. Therefore, a number of studies have proposed that homogenous bone marrow stem cells can be generated from clonal culture of bone marrow cells and that BMSC clones have the greatest potential for the application of bone regeneration in vivo. PMID:21125790

Xiao, Yin; Mareddy, Shobha; Crawford, Ross

2010-01-01

364

Adhesive and mechanical regulation of mesenchymal stem cell differentiation in human bone marrow and  

E-print Network

as a critical cell source in tissue engineering, which involves the ex vivo creation of biological implantsAdhesive and mechanical regulation of mesenchymal stem cell differentiation in human bone marrow can influence commitment of human bone marrow-derived mesenchymal stem cells (hBMCs) to adipogenic

Chen, Christopher S.

365

Validation of noninvasive quantification of bone marrow fat volume with microCT in aging rats  

Microsoft Academic Search

Marrow fat infiltration is one of the hallmarks of age-related bone loss. This fat infiltration has been quantified by invasive and noninvasive methods. However, the validity of the noninvasive methods has not been correlated with a gold standard. In this study we aim to validate the usefulness of marrow fat quantification by correlating microCT (?CT) images with histology analysis. Fat

Oddom Demontiero; Wei Li; Emma Thembani; Gustavo Duque

2011-01-01

366

Diagnosis and clinical associations of zinc depletion following bone marrow transplantation  

Microsoft Academic Search

Following the emergence of biochemical zinc deficiency after bone marrow transplantation, the clinical value of plasma alkaline phosphatase activity as an early indicator of biochemical zinc depletion was investigated in this group of patients. Serial measurements of plasma zinc and alkaline phosphatase activities in 28 consecutive children (median age 8.7 years; 16 males) undergoing bone marrow transplantation were carried out

A Papadopoulou; K Nathavitharana; M D Williams; P J Darbyshire; I W Booth

1996-01-01

367

Bone Marrow Cell Recruitment to the Brain in the Absence of Irradiation or Parabiosis Bias  

PubMed Central

The engraftment of bone marrow-derived cells has been described not only during diseases of the central nervous system (CNS) but also under healthy conditions. However, previous studies pointing to an ample bone marrow cell engraftment used irradiation-induced bone marrow chimeras that evoked severe alterations of the CNS micromilieu including disturbances of the blood brain barrier (BBB), damage of endothelial cells and local induction of proinflammatory cytokines. On the other hand, parabiosis experiments using temporarily joined circulatory systems generally yielded low levels of myeloid cell chimerism thereby potentially underestimating bone marrow cell turnover with the CNS. To avoid these drawbacks we established a protocol using the alkylating agent busulfan prior to allogenic bone marrow transplantation from CX3CR1GFP/+ donors. This regimen resulted in a stable and high peripheral myeloid chimerism, significantly reduced cytokine induction and preserved BBB integrity. Importantly, bone marrow cell recruitment to the CNS was strongly diminished under these conditions and only weakly enhanced during local neurodegeneration induced by facial nerve axotomy. These results underscore the requirement of local CNS conditioning for efficient recruitment of bone marrow cells, establish busulfan as an alternative treatment for studying bone marrow chimeras and suggest a critical re-evaluation of earlier chimeric studies involving irradiation or parabiosis regimens. PMID:23526995

Kierdorf, Katrin; Katzmarski, Natalie; Haas, Carola A.; Prinz, Marco

2013-01-01

368

Combination therapies prevent the neuropathic, proinflammatory characteristics of bone marrow in streptozotocin-induced diabetic rats.  

PubMed

We previously showed that peripheral neuropathy of the bone marrow was associated with loss of circadian rhythmicity of stem/progenitor cell release into the circulation. Bone marrow neuropathy results in dramatic changes in hematopoiesis that lead to microvascular complications, inflammation, and reduced endothelial repair. This series of events represents early pathogenesis before development of diabetic retinopathy. In this study we characterized early alterations within the bone marrow of streptozotocin (STZ)-induced diabetic rats following treatments that prevent experimental peripheral neuropathy. We asked whether bone marrow neuropathy and the associated bone marrow pathology were reversed with treatments that prevent peripheral neuropathy. Three strategies were tested: inhibition of neutral endopeptidase, inhibition of aldose reductase plus lipoic acid supplementation, and insulin therapy with antioxidants. All strategies prevented loss of nerve conduction velocity resulting from STZ-induced diabetes and corrected the STZ-induced diabetes-associated increase of immunoreactivity of neuropeptide Y, tyrosine hydroxylase, and somatostatin. The treatments also reduced concentrations of interleukin-1?, granulocyte colony-stimulating factor, and matrix metalloproteinase 2 in STZ-induced diabetic bone marrow supernatant and decreased the expression of NADPH oxidase 2, nitric oxide synthase 2, and nuclear factor-?B1 mRNA in bone marrow progenitor cells. These therapies represent novel approaches to attenuate the diabetic phenotype within the bone marrow and may constitute an important therapeutic strategy for diabetic microvascular complications. PMID:25204979

Dominguez, James M; Yorek, Mark A; Grant, Maria B

2015-02-01

369

Clinical responses to bone marrow transplantation in children with severe osteogenesis imperfecta  

Microsoft Academic Search

Preclinical models have shown that trans- plantation of marrow mesenchymal cells has the potential to correct inherited dis- orders of bone, cartilage, and muscle. The report describes clinical responses of the first children to undergo allogeneic bone marrow transplantation (BMT) for severe osteogenesis imperfecta (OI), a genetic disorder characterized by defec- tive type I collagen, osteopenia, bone fragility, severe bony

Edwin M. Horwitz; Darwin J. Prockop; Patricia L. Gordon; Winston W. K. Koo; Lorraine A. Fitzpatrick; Michael D. Neel; M. Elizabeth McCarville; Paul J. Orchard; Reed E. Pyeritz; Malcolm K. Brenner

2010-01-01

370

Hepatic Parenchymal Replacement in Mice by Transplanted Allogeneic Hepatocytes Is Facilitated by Bone Marrow  

E-print Network

with allogeneic FVB-derived bone marrow. These findings were validated by a comparison of hAAT levels betweenHepatic Parenchymal Replacement in Mice by Transplanted Allogeneic Hepatocytes Is Facilitated by Bone Marrow Transplantation and Mediated by CD4 Cells Konrad L. Streetz,1,5 Regis Doyonnas,2 Dirk Grimm

Ford, James

371

Cell fusion of bone marrow cells and somatic cell reprogramming by embryonic stem cells  

PubMed Central

Bone marrow transplantation is a curative treatment for many diseases, including leukemia, autoimmune diseases, and a number of immunodeficiencies. Recently, it was claimed that bone marrow cells transdifferentiate, a much desired property as bone marrow cells are abundant and therefore could be used in regenerative medicine to treat incurable chronic diseases. Using a Cre/loxP system, we studied cell fusion after bone marrow transplantation. Fused cells were chiefly Gr-1+, a myeloid cell marker, and found predominantly in the bone marrow; in parenchymal tissues. Surprisingly, fused cells were most abundant in the kidney, Peyer’s patches, and cardiac tissue. In contrast, after cell fusion with embryonic stem cells, bone marrow cells were reprogrammed into new tetraploid pluripotent stem cells that successfully differentiated into beating cardiomyocytes. Together, these data suggest that cell fusion is ubiquitous after cellular transplants and that the subsequent sharing of genetic material between the fusion partners affects cellular survival and function. Fusion between tumor cells and bone marrow cells could have consequences for tumor malignancy.—Bonde, S., Pedram, M., Stultz, R., Zavazava, N. Cell fusion of bone marrow cells and somatic cell reprogramming by embryonic stem cells. PMID:19762558

Bonde, Sabrina; Pedram, Mehrdad; Stultz, Ryan; Zavazava, Nicholas

2010-01-01

372

Bone marrow edema of the mandibular condyle related to internal derangement, osteoarthrosis, and joint effusion  

Microsoft Academic Search

Purpose: The purpose of this prospective study was to evaluate whether common magnetic resonance imaging (MRI) variables such as temporomandibular joint (TMJ) internal derangement, osteoarthrosis, and effusion may predict the diagnostic group of bone marrow edema of the mandibular condyle. Materials and Methods: The relationship between bone marrow edema and TMJ disc displacement, osteoarthrosis, and effusion was analyzed in MRIs

Iris Brandlmaier; Christoph Schmid; Stefan Bertram; Ansgar Rudisch

2003-01-01

373

Prevalence of bone marrow signal abnormalities observed in the temporomandibular joint using magnetic resonance imaging  

Microsoft Academic Search

aPurpose The purpose of this investigation was to determine the prevalence of bone marrow signal abnormalities in patients referred for temperomandibular joint (TMJ) magnetic resonance imaging (MRI). This investigation was done because of prior studies suggesting that condylar marrow signal abnormalities indicate avascular necrosis.Subjects and Methods Retrospective review was done of 449 consecutive TMJ MR examinations in 415 patients from

James M Lieberman; Catherine L Gardner; Antonino O Motta; Robert D Schwartz

1996-01-01

374

Defense Health Program DEPARTMENT OF DEFENSE BONE MARROW FAILURE RESEARCH PROGRAM  

E-print Network

Defense Health Program DEPARTMENT OF DEFENSE BONE MARROW FAILURE RESEARCH PROGRAM FUNDING OPPORTUNITIES FOR FISCAL YEAR 2013 The Department of Defense Bone Marrow Failure Research Program (BMFRP of Congressionally Directed Medical Research Programs (CDMRP). The FY13 Defense Appropriations Act provides $3

von der Heydt, Rüdiger

375

Genomic analysis of bone marrow failure and myelodysplastic syndromes reveals phenotypic and diagnostic complexity.  

PubMed

Accurate and timely diagnosis of inherited bone marrow failure and inherited myelodysplastic syndromes is essential to guide clinical management. Distinguishing inherited from acquired bone marrow failure/myelodysplastic syndrome poses a significant clinical challenge. At present, diagnostic genetic testing for inherited bone marrow failure/myelodysplastic syndrome is performed gene-by-gene, guided by clinical and laboratory evaluation. We hypothesized that standard clinically-directed genetic testing misses patients with cryptic or atypical presentations of inherited bone marrow failure/myelodysplastic syndrome. In order to screen simultaneously for mutations of all classes in bone marrow failure/myelodysplastic syndrome genes, we developed and validated a panel of 85 genes for targeted capture and multiplexed massively parallel sequencing. In patients with clinical diagnoses of Fanconi anemia, genomic analysis resolved subtype assignment, including those of patients with inconclusive complementation test results. Eight out of 71 patients with idiopathic bone marrow failure or myelodysplastic syndrome were found to harbor damaging germline mutations in GATA2, RUNX1, DKC1, or LIG4. All 8 of these patients lacked classical clinical stigmata or laboratory findings of these syndromes and only 4 had a family history suggestive of inherited disease. These results reflect the extensive genetic heterogeneity and phenotypic complexity of bone marrow failure/myelodysplastic syndrome phenotypes. This study supports the integration of broad unbiased genetic screening into the diagnostic workup of children and young adults with bone marrow failure and myelodysplastic syndromes. PMID:25239263

Zhang, Michael Y; Keel, Siobán B; Walsh, Tom; Lee, Ming K; Gulsuner, Suleyman; Watts, Amanda C; Pritchard, Colin C; Salipante, Stephen J; Jeng, Michael R; Hofmann, Inga; Williams, David A; Fleming, Mark D; Abkowitz, Janis L; King, Mary-Claire; Shimamura, Akiko

2015-01-01

376

Cryptococcososis of Bone Marrow: A Case Report with Review of Literature  

PubMed Central

Cryptococcus has been increasingly seen as a pathogen with the growing incidence of Acquired Immune Deficiency Syndrome (AIDS). Cryptococcosis commonly presents with pulmonary, CNS or skin involvement. Bone marrow involvement of Cryptococci has been rarely reported. We are reporting a case of bone marrow Cryptococcosis in a 50–year–old Human Immune Deficiency Virus (HIV) positive male, with review of literature. PMID:24596754

B.R, Ashwini; Raghupathi, AR; A., Srinarthan

2014-01-01

377

Donor-Derived, Liver-Specific Protein Expression after Bone Marrow Transplantation  

E-print Network

Donor-Derived, Liver-Specific Protein Expression after Bone Marrow Transplantation D. Denison mechanism to deliver a functional gene to a deficient liver. Bone marrow-derived hepatocytes are rare and without a defined contribution to liver function. Con- sequently, the clinical significance of BMT

Ford, James

378

The predicting role of substance P in the neoplastic transformation of the hypoplastic bone marrow  

PubMed Central

Aims To estimate the expression of substance P in the haematopoietic cells of hypoplastic bone marrow and define its relationship with the course of bone marrow hypoplasia. Methods Bone marrow specimens were obtained from 42 children with bone marrow hypoplasia who were hospitalised in the Department of Pediatric Oncology, Hematology and Transplantology, Poznan University of Medical Sciences, Poznan, Poland, between 1996 and 2003. Substance P and Ki?67 expression were evaluated using immunochemical and hybridocytochemical assays. Results The expression of substance P (as evidenced by both immunocytochemical and hybridisation techniques) was confirmed in the cytoplasm of B lymphocytes in 8 of 11 children who developed acute leukaemia in 45 (SD 12)?days. The percentage of substance P?positive cells ranged from 67.6 to 95.8 (mean of 81.5% cells with immunocytochemistry and 84.3% with in situ hybridisation). The risk of development of leukaemia secondary to bone marrow hypoplasia was found to be significant (p<0.001) in those children who expressed substance P in normal?looking lymphocytes at the initial bone marrow evaluation. Conclusions The presence of substance P in B lymphocytes of hypoplastic bone marrow may predict its neoplastic transformation. A marked correlation between substance P?positive bone marrow pattern and the expansion of tumour cells may prove the potential value of this oligopeptide in the pathogenesis of leukaemia. PMID:16935970

Nowicki, M; Ostalska?Nowicka, D; Konwerska, A; Miskowiak, B

2006-01-01

379

Bone Marrow Involvement in Neuroblastoma: A Study of Hemato-morphological Features.  

PubMed

Bone marrow involvement in neuroblastoma indicates advanced stage of disease. The recent use of autologous bone marrow "rescue", has provided an additional important reason for accurate assessment of bone marrow status in newly diagnosed patients. In this study, we analyzed 44 cases of neuroblastoma for bone marrow infiltration status and their hematological parameters. Eighty-eight bone marrow aspirate and trephine touch imprint smears and 44 trephine biopsy sections were examined in these 44 patients. Of these, 24 cases (54.5 %) showed marrow infiltration. Leucopenia and bicytopenia were significantly (p < 0.05) associated with marrow infiltration. Both bone marrow aspirate and biopsy were positive for infiltration in 16 out of 24 positive cases. Only aspirate smears were positive in 4 and only trephine biopsy in another 4 cases. The pattern of infiltration consisted of rosette formation in 40.7 % cases on aspirate smears and 22.2 % cases in trephine biopsies. Remaining cases showed diffuse and interstitial presence of tumor cells and cases positive only on trephine biopsy, showed marked stromal reaction. Bilateral trephine biopsies combined with aspirate smears picked up all positive cases compared to when they were assessed alone. PMID:25548446

Rastogi, Pulkit; Naseem, Shano; Varma, Neelam; Das, Reena; Ahluwalia, Jasmina; Sachdeva, Man Updesh Singh; Sharma, Prashant; Kumar, Narender; Marwaha, Ram Kumar

2015-03-01

380

Autologous Peripheral Hematopoietic Stem Cell Transplantation Restores Hematopoietic Function Following Marrow Ablative Therapy  

Microsoft Academic Search

From ten patients with advanced malignant disease involv- ing the bone marrow, autologous hematopoietic stem cells were collected from the peripheral blood during eight four-hour pheresis procedures and cryopreserved. No manipulations to increase the number of stem cells circu- lating in the blood were used during the collections. Follow- ing marrow ablative chemotherapy or chemoradiotherapy. the autologous cells were thawed

Anne Kessinger; James D. Landmark; Douglas M. Smith; Dennis D. Weisenburger

1988-01-01

381

Fresh Bone Marrow and Periosteum Transplantation for Cartilage Defects of the Knee  

Microsoft Academic Search

The aim of work was to analyze clinical effectiveness of fresh bone marrow and periosteum transplantation in the treatment of traumatic or degenerative cartilage defects. The 14 patients in this study had a mean age of 37 years. Bone marrow aspirated from the iliac crest was implanted under the periosteum sutured over the defect. Three, 6, and 12 months postoperatively,

K. Slynarski; J. Deszczynski; J. Karpinski

2006-01-01

382

Familial, structural, and environmental correlates of MRI-defined bone marrow lesions: a sibpair study  

Microsoft Academic Search

The aim of this study was to estimate the heritability and describe the correlates of bone marrow lesions in knee subchondral bone. A sibpair design was used. T2- and T1-weighted MRI scans were performed on the right knee to assess bone marrow lesions at lateral tibia and femora and medial tibia and femora, as well as chondral defects. A radiograph

Guangju Zhai; James Stankovich; Flavia Cicuttini; Changhai Ding; Graeme Jones

2006-01-01

383

MRI of bone marrow in the distal radius: in vivo precision of effective transverse relaxation times  

Microsoft Academic Search

The effective transverse relaxation time T2* is influenced by the presence of trabecular bone, and can potentially provide a measure of bone density as well as bone structure. We determined the in vivo precision of T2* in repeated bone marrow measurements. The T2* measurements of the bone marrow of the distal radius were performed twice within 2 weeks in six

Stephan Grampp; Sharmila Majumdar; Michael Jergas; Philipp Lang; Alice Gies; Harry K. Genant

1995-01-01

384

Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development  

Microsoft Academic Search

BACKGROUND: Increased bone marrow lipid deposition in steroid-associated osteonecrosis (ON) implies that abnormalities in fat metabolism play an important role in ON development. The increase in lipid deposition might be explained by elevated adipogenesis of marrow mesenchymal stem cells (MSCs). However, it remains unclear whether there is a close association between elevated adipogenesis and steroid-associated ON development. OBJECTIVE: The present

Hui H Sheng; Ge G Zhang; Wing Hoi Cheung; Chun Wai Chan; Yi Xiang Wang; Kwong Man Lee; Hong Fu Wang; Kwok Sui Leung; Ling L Qin

2007-01-01

385

Immobilized sonic hedgehog N-terminal signaling domain enhances differentiation of bone marrow-derived  

E-print Network

Immobilized sonic hedgehog N-terminal signaling domain enhances differentiation of bone marrow: The signaling domain of Sonic hedgehog (Shh), a potent upstream regulator of cell fate that has been implicated­1208, 2007 Key words: sonic hedgehog; bone marrow-derived mesen- chymal stem cells; biomimetic; biointerface

Schaffer, David V.

386

TOXICITY OF TRIGONELLA FOENUM GRAECUM (FENUGREEK) IN BONE MARROW CELL PROLIFERATION IN RAT  

Microsoft Academic Search

Fenugreek has a wide range of medical applications and its medicinal use has been clear in several studies, however, few studies are available on effects on haematopoietic stem cell of bone marrow. The goal of the present study was to investigate the effect of Fenugreek on fetal macroscopic diameters and microscopic bone marrow cell histological changes in its teratogenic dosages.

M. ARAEE; M. NOROUZI; G. HABIBI; M. SHEIKHVATAN

2009-01-01

387

Transplantation of hypoxia preconditioned bone marrow mesenchymal stem cells enhances angiogenesis and neurogenesis after cerebral ischemia in rats  

E-print Network

Transplantation of hypoxia preconditioned bone marrow mesenchymal stem cells enhances angiogenesis online 9 March 2012 Keywords: Hypoxic preconditioning Bone marrow mesenchymal stem cell Transplantation for promoting cell survival after transplantation. The present investigation examined the hypothesis

Hayar, Abdallah

388

Bone marrow lesion volume reduction is not associated with improvement of other periarticular bone measures: data from the Osteoarthritis Initiative  

E-print Network

A: MRI-detected subchondral bone marrow signal alterationsMRI-detected subchondral bone marrow lesions is associatedmarrow lesion (BML) volume change and changes in periarticular bone mineral density (paBMD) as well as subchondral

2013-01-01

389

Red Studio - MoMA  

NSDL National Science Digital Library

By collaborating with high school students, the Museum of Modern Art (MoMA) put together Red Studio, a Web site designed to connect teens with modern art and today's working artists. Currently, Red Studio features an interview with Shahzia Sikander, an artist born in Pakistan in 1969, who was educated, and now lives here in the US. Conducted by six students, interview questions range from what it's like for a young woman with a Muslim family to pursue a career as an artist, if she's ever felt she has to censor her art, to what type of music she likes. Red Studio visitors can view the interview as a Flash presentation with sound, or read the complete transcript. There is also an earlier interview with Vito Acconci, who is asked if he is an artist or an architect, and why he always wears black. Another teen-orientated part of the site is polls, so that kids can find out what other kids think about the purpose of art, and what they like to do after school.

2005-01-01

390

One antigen mismatched related donor bone marrow transplant in a patient with acute lymphoblastic leukaemia and beta-thalassaemia major: potential cure of both marrow disorders.  

PubMed

We report a case of a 34-year-old man with T-ALL and beta-thalassaemia major who underwent a one antigen mismatched related donor bone marrow transplant. Five months post transplant chimeric studies revealed full donor haemopoiesis and the patient remains leukaemia and thalassaemia free at 12 months post transplant. Cumulative risk factors contributing to the increased transplant-related mortality in patients with two different marrow disorders are discussed. PMID:10734304

Kottaridis, P D; Peggs, K; Lawrence, A; Verfuerth, S; Chatterjee, R; Telfer, P; Porter, J B; Mackinnon, S; Goldstone, A H

2000-03-01

391

Argyrophilic nucleolar organiser region (AgNOR) staining in normal bone marrow cells.  

PubMed Central

Fifteen normal bone marrow aspirates were stained with the agyrophilic nucleolar organiser region (AgNOR) method. The results of the specific staining AgNORs as well as nuclear and cytoplasmic staining were analysed. A system was devised to characterise precisely the AgNORs present in the nuclei of bone marrow cells. Particular types of bone marrow cells had a characteristic AgNOR and non-AgNOR staining pattern. The bone marrow cells were identified easily and reliably with AgNOR staining and the method was especially useful for lymphocytes, plasma cells, erythroid cells, basophils/mast cells, monocytes and cells containing haemosiderin. The immature haemopoietic cells exhibited more and larger AgNORs than the more mature cells. It is concluded that AgNOR staining can be used to study bone marrow cells by providing additional information when used in conjunction with conventional stains. Images PMID:1698824

Nikicicz, E P; Norback, D H

1990-01-01

392

Giant hepatic adenoma with bone marrow metaplasia not associated with oral contraceptive intake  

PubMed Central

Background Hepatocellular adenomas are the most common benign liver tumors. They are usually related to oral contraceptive intake. Case presentation This case describes a 58-year-old woman admitted to our institution for a hepatic mass incidentally discovered during a routine examination. The patient, who was never on oral contraceptives, was asymptomatic upon admission. She underwent a thorough diagnostic evaluation and then a hepatic right trisegmentectomy. The histologic evaluation of the mass showed that it was a hepatocellular adenoma with areas of bone marrow metaplasia. Conclusion Bone marrow metaplasia has rarely been found associated to liver tumors. The presence of marrow-derived hepatic progenitor cells might be the source of both adenoma hepatocytes and bone marrow differentiated cells. To our knowledge, this is only the second case in the English literature in which areas of bone marrow metaplasia were found in a hepatocellular adenoma. PMID:16934137

Ramacciato, Giovanni; Nigri, Giuseppe R; Aurello, Paolo; D'Angelo, Francesco; Pezzoli, Francesca; Rossi, Simone; Pilozzi, Emanuela; Ercolani, Giorgio; Ravaioli, Matteo

2006-01-01

393

Detection of paroxysmal nocturnal hemoglobinuria clones to exclude inherited bone marrow failure syndromes  

PubMed Central

Background Inherited bone marrow failure syndromes (IBMFS) and acquired aplastic anemia (AA) are life-threatening marrow failure disorders. These entities can be difficult to distinguish because they present similarly. Correct diagnosis is imperative for proper therapy. Design and methods This is a retrospective, single-center study of patients <40 yr of age, evaluated for bone marrow failure, and assayed for the presence of a PNH clone in the pediatric or adult hematology/oncology clinics from 2001 to present. Patients were also evaluated for IBMFS. Results We present results from 156 patients with marrow failure, 20 of whom have IBMFS. None of the IBMSF patients had paroxysmal nocturnal hemoglobinuria (PNH) clones. Conclusions Although further studies are needed, our results suggest that the detection of a PNH clone can be a useful diagnostic tool to exclude the diagnosis of IBMFS and focus the work-up and treatment on an acquired form of marrow failure. PMID:24612308

DeZern, Amy E.; Symons, Heather J.; Resar, Linda S.; Borowitz, Michael J.; Armanios, Mary Y.; Brodsky, Robert A.

2014-01-01

394

[The role of the bone marrow in forming an immunological memory to Staphylococcus].  

PubMed

The dynamic study of antibody formation in the bone marrow tissue of mice immunized with corpuscular staphylococcal antigen was made. A single intravenous injection of this antigen to the animals produced no essential changes in the number of antibody-forming cells in the bone marrow, their repeated immunization led to the involvement of the bone marrow in the anamnetic reaction, which was manifested by the accumulation of a great number of plasmocytes synthesizing antibodies to staphylococci in bone marrow tissue. The relative contribution of the bone marrow to systemic antibody formation essentially increased at later stages of secondary immune response. The possible biological significance of the regularities and the practical importance of the data revealed in this investigation are discussed. PMID:2711792

Bobrovnik, S A; Liashchenko, K P

1989-01-01

395

Engraftment of a Clonal Bone Marrow Stromal Cell Line in vivo Stimulates Hematopoietic Recovery from Total Body Irradiation  

Microsoft Academic Search

Whether bone marrow stromal cells of donors contribute physiologically to hematopoietic stem cell reconstitution after marrow transplantation is unknown. To determine the transplantability of nonhematopoietic marrow stromal cells, stable clonal stromal cell line (GB1\\/6) expressing the a isoenzyme of glucose-6-phosphate isomerase (Glu6PI-a, D-glucose-6-phosphate ketol-isomerase; EC 5.3.1.9) was derived from murine long-term bone marrow cultures and made resistant to neomycin analogue

Pervin Anklesaria; Kenneth Kase; Julie Glowacki; Christie A. Holland; Mary Ann Sakakeeny; Jocyndra A. Wright; T. J. Fitzgerald; Chi-Yu Lee; Joel S. Greenberger

1987-01-01

396

Development of acute pure red cell aplasia after deferasirox administration in two cases of myelodysplastic syndrome.  

PubMed

Cases 1 and 2 were 55- and 68-year-old males, respectively. Both were administered deferasirox (DFX) because they received red blood cell transfusions regularly as treatment for myelodysplastic syndrome refractory anemia. DFX administrations were stopped on the 22nd day in case 1 and on the 78th day in case 2 because significantly reduced hemoglobin values and reticulocyte counts were observed. Bone marrow examinations showed pure red cell aplasia in both cases. In case 1, the reticulocyte ratio recovered to the value before drug administration 21 days after drug withdrawal. In case 2, it started increasing on the 14th day, and had recovered to the value before drug administration by the 42nd day after drug withdrawal. Human parvovirus B19 infections were negative in both cases. Both cases were thought to have drug-induced pure red cell aplasia, probably due to DFX. This drug should be used carefully with regular follow-ups of the reticulocyte count. PMID:24850456

Hayakawa, Fumihiko; Tomita, Akihiro; Naoe, Tomoki

2014-04-01

397

Rheological behavior of fresh bone marrow and the effects of storage.  

PubMed

The progression of several diseases, such as osteoporosis and diabetes, are associated with changes in marrow composition and physiology. As these diseases are affected by aging and activity, the biomechanical properties and mechanobiology of marrow may play a role in their progression. Bone marrow is comprised primarily of cells, and provides a niche for several mechanosensitive cell lineages. The mechanical signals imparted to the cells depend on their interaction with one another, the extracellular matrix, and the intercellular fluid. At a macroscopic scale, these interactions manifest as viscosity in marrow. Marrow viscosity has been measured in human and bovine bone. However, a large range of storage, retrieval, and measurement techniques has resulted in inconsistent data. To provide physiologically relevant data, marrow samples from young adult pigs were harvested and tested within less than 8h of slaughter. The viscosity was over 100Pas at a shear rate of 1s(-1), and decreased with shear rate according to a power law. However, the marrow did not exhibit a measurable yield stress as some complex fluids do. The viscosity of samples that had been frozen and thawed prior to testing was lower by an order of magnitude. The difference in properties was associated with a loss of integrity of the marrow adipocyte membranes. Previous reports of bone marrow viscosity have shown inconsistent results, which may be due to different storage and handling prior to testing. The higher viscosity compared to previous reports would impact poroelastic models of bone, and suggests that the stress on marrow cells during whole bone loading may be higher than previously believed. PMID:25262201

Metzger, Thomas A; Shudick, Jonelle M; Seekell, Raymond; Zhu, Yingxi; Niebur, Glen L

2014-12-01

398

The In Situ Mechanics of Trabecular Bone Marrow: The Potential for Mechanobiological Response.  

PubMed

Bone adapts to habitual loading through mechanobiological signaling. Osteocytes are the primary mechanical sensors in bone, upregulating osteogenic factors and downregulating osteoinhibitors, and recruiting osteoclasts to resorb bone in response to microdamage accumulation. However, most of the cell populations of the bone marrow niche, which are intimately involved with bone remodeling as the source of bone osteoblast and osteoclast progenitors, are also mechanosensitive. We hypothesized that the deformation of trabecular bone would impart mechanical stress within the entrapped bone marrow consistent with mechanostimulation of the constituent cells. Detailed fluid-structure interaction models of porcine femoral trabecular bone and bone marrow were created using tetrahedral finite element meshes. The marrow was allowed to flow freely within the bone pores, while the bone was compressed to 2000 or 3000 microstrain at the apparent level. Marrow properties were parametrically varied from a constant 400 mPa·s to a power-law rule exceeding 85?Pa·s. Deformation generated almost no shear stress or pressure in the marrow for the low viscosity fluid, but exceeded 5?Pa when the higher viscosity models were used. The shear stress was higher when the strain rate increased and in higher volume fraction bone. The results demonstrate that cells within the trabecular bone marrow could be mechanically stimulated by bone deformation, depending on deformation rate, bone porosity, and bone marrow properties. Since the marrow contains many mechanosensitive cells, changes in the stimulatory levels may explain the alterations in bone marrow morphology with aging and disease, which may in turn affect the trabecular bone mechanobiology and adaptation. PMID:25363343

Metzger, Thomas A; Kreipke, Tyler C; Vaughan, Ted J; McNamara, Laoise M; Niebur, Glen L

2015-01-01

399

Bone Marrow Transplantation, (1997) 19, 315322 1997 Stockton Press All rights reserved 02683369/97 $12.00  

E-print Network

Bone Marrow Transplantation, (1997) 19, 315­322 © 1997 Stockton Press All rights reserved 0268 and autologous bone marrow transplantation M Elkordy, M Crump, JJ Vredenburgh, WP Petros, A Hussein, P Rubin, M Peters Duke University, Bone Marrow Transplant Program, Durham, NC, USA Summary: The use of lineage

Rabinowitz, Joshua D.

400

Injections of leptin into rat ventromedial hypothalamus increase adipocyte apoptosis in peripheral fat and in bone marrow  

Microsoft Academic Search

The accumulation of fat cells (adipocytes) in bone marrow is now thought to be a factor contributing to age-related bone loss. Women with osteoporosis have higher numbers of marrow adipocytes than women with healthy bone, and bone formation rate is inversely correlated with adipocyte number in bone tissue biopsies from both men and women. Adipogenic differentiation of bone marrow stromal

Mark W. Hamrick; Mary Anne Della Fera; Yang-Ho Choi; Diane Hartzell; Catherine Pennington; Clifton A. Baile

2007-01-01

401

Successful adoptive immunotherapy of minimal residual disease after chemoradiotherapy and transplantation of bone marrow purged of leukaemia with mafosfamide  

Microsoft Academic Search

Summary The effectiveness of adoptive immunotherapy in eliminating minimal residual disease in tumour-bearing mice after bone marrow transplantation was tested. This model mimics the human clinical condition when autologous bone marrow was purged ex vivo of leukaemia with mafosfamide or was not purged, and stored in liquid nitrogen before transplantation. Animals with minimal residual disease were prepared with marrow-ablative but

Tomasz Skórski; Maciej Kawalec; Jerzy Kawiak

1990-01-01

402

Accumulation of low molecular weight (bleomycin detectable) iron in bone marrow cells of rats after benzene exposure  

Microsoft Academic Search

An accumulation of low molecular weight (LMW) bleomycin detectable iron in the bone marrow was observed after administration of benzene (IP 0.5 ml\\/kg, daily) for 5 and 10 days in female albino rats. However, this LMW iron was not detectable in the bone marrow of rats from the control group. Studies of bone marrow fractionation showed that the maximum accumulation

K. P. Pandya; G. S. Rao; S. Khan; R. Krishnamurthy

1990-01-01

403

Effect of chitosan particles and dexamethasone on human bone marrow stromal cell osteogenesis and angiogenic factor secretion  

E-print Network

Effect of chitosan particles and dexamethasone on human bone marrow stromal cell osteogenesis 2009 Edited by: J. Aubin Keywords: Angiogenesis Bone marrow stromal cells Chitin/chitosan Dexamethasone bone marrow stimulation, and it is reported to increase angiogenesis and osteogenesis in vivo. Here, we

Buschmann, Michael

404

Bone Marrow Stimulation of the Medial Femoral Condyle Produces Inferior Cartilage and Bone Repair Compared to the Trochlea in a  

E-print Network

Bone Marrow Stimulation of the Medial Femoral Condyle Produces Inferior Cartilage and Bone Repair femoral condylar (MFC) versus femoral trochlear (TR) defects 3 months after bone marrow stimulation histomorphom- etry and histological scoring showed that bone marrow stimulation produced inferior soft tissue

Buschmann, Michael

405

Late effects in children after bone marrow transplantation: a review.  

PubMed

Since the number of children receiving a bone marrow transplantation (BMT) and becoming long-term survivors continues to increase, more attention has to be paid to detect long-term side effects in these unique patients. Follow-up studies to timely identify these untoward sequelae are a matter of particular concern for pediatricians due to the longer life expectancy of children cured by BMT. The more frequently recognized sequelae affecting lung, eyes, brain and the endocrine system have been analyzed in this review. The majority of long-term side effects could be related to the conditioning regimens employed to prepare children before marrow transplantation and radiotherapy has been indicated as the most important agent determining deleterious toxicities. Most children receiving BMT present a decreased growth velocity and this growth impairment is especially observed in patients receiving total body irradiation (TBI) and prophylactic cranial irradiation prior to marrow transplant. Growth hormone deficiency could be demonstrated in the majority of patients with a reduced growth rate, even though an impairment of liver somatomedin production or a direct radiation-induced skeletal dysplasia could not be excluded. Overt and compensated hypothyroidism have been reported after TBI and patients given single dose radiotherapy are at greater risk with an overall incidence of thyroid function abnormalities approaching 30-40%. Delayed puberty development was reported in boys and girls after a TBI-containing conditioning regimen, whereas patients given BMT for severe aplastic anaemia presented a normal puberty. The absence of pubertal growth spurt contributes to the growth impairment of prepubertal children. In post-pubertal patients amenorrhea, azoospermia and gonadal failure can be observed after radiotherapy and several patients can require hormonal substitutive therapy. Skin and mucosal abnormalities referred to teguments involvement by chronic graft-versus-host disease (GVHD). Moreover, alopecia or abnormal pigmentation of the skin are observed in patients given busulfan as part of their myeloablative therapy. Cataracts are a well recognized complication of children receiving ionizing radiations and chronic steroid therapy. Again, posterior subcapsular cataracts occur more frequently in patients given TB1 as single exposure. Decreased lacrimal gland function, with impairment of tear production is another late effect of irradiation to the eye. Lung function abnormalities are not rare after transplant and may cause late mortality and morbidity.(ABSTRACT TRUNCATED AT 400 WORDS) PMID:8314162

Locatelli, F; Giorgiani, G; Pession, A; Bozzola, M

1993-01-01

406

Pure red-cell aplasia following major and bi-directional ABO-incompatible allogeneic stem-cell transplantation: recovery of donor-derived erythropoiesis after long-term treatment using different therapeutic strategies  

Microsoft Academic Search

Blood group incompatibility between donor and recipient of allogeneic stem cell transplants may be associated with post-transplant\\u000a erythroid aplasia. A total of 548 patients (pts) received allogeneic transplant for malignant and non-malignant hematologic\\u000a disorders. In a retrospective analysis, the prevalence and outcome of pure red-cell aplasia (PRCA) in 44 pts with major and\\u000a bi-directional ABO-mismatch were investigated. Bone marrow grafts

Grzegorz Helbig; Beata Stella-Holowiecka; Jerzy Wojnar; Malgorzata Krawczyk; Slawomira Krzemien; Maria Wojciechowska-Sadus; Miros?aw Markiewicz; Iwona Wylezol; Malgorzata Kopera; Jerzy Holowiecki

2007-01-01

407

Bone-Marrow-Derived Mesenchymal Stem Cells for Organ Repair  

PubMed Central

Mesenchymal stem cells (MSCs) are prototypical adult stem cells with the capacity for self-renewal and differentiation with a broad tissue distribution. MSCs not only differentiate into types of cells of mesodermal lineage but also into endodermal and ectodermal lineages such as bone, fat, cartilage and cardiomyocytes, endothelial cells, lung epithelial cells, hepatocytes, neurons, and pancreatic islets. MSCs have been identified as an adherent, fibroblast-like population and can be isolated from different adult tissues, including bone marrow (BM), umbilical cord, skeletal muscle, and adipose tissue. MSCs secrete factors, including IL-6, M-CSF, IL-10, HGF, and PGE2, that promote tissue repair, stimulate proliferation and differentiation of endogenous tissue progenitors, and decrease inflammatory and immune reactions. In this paper, we focus on the role of BM-derived MSCs in organ repair. PMID:23554816

Li, Ming; Ikehara, Susumu

2013-01-01

408

Bone marrow Schwann cells induce hematopoietic stem cell hibernation.  

PubMed

Hematopoietic stem cells (HSCs) are clonogenic cells capable of both self-renewal and multilineage differentiation. In adult mouse bone marrow (BM), most HSCs remain in the non-dividing G0-phase of cell cycle, in close contact with supporting cells known as the HSC "niche". In the present study, we focused on signaling mechanisms that regulate stem cell dormancy in the BM niche. We show that TGF-? type II receptor deficiency causes reduced phosphorylation of Smad2/3 and impairs long-term repopulating activity in HSCs, suggesting a significant role for TGF-?/Smad signaling in hematopoiesis. Furthermore, we aimed at defining the candidate BM niche responsible for homeostasis of hematopoiesis, and revealed that non-myelinating Schwann cells sustain HSC hibernation by converting TGF-? from its latent to its active form. PMID:24817152

Yamazaki, Satoshi; Nakauchi, Hiromitsu

2014-06-01

409

Bone marrow stem cell imaging after intracoronary administration.  

PubMed

Although feasibility and safety of autologous stem cells administration to the post-infarction heart has been proven it is not known what proportion of cells effectively do home at the damaged site. Therefore, we have labeled autologous bone marrow cells (ABMC's) by radioactive Indium and single photon emission computed tomography (SPECT) tissue distribution has been analyzed. It was detected that up to 10% of the cells were retained within the myocardium while their majority migrated or has been anchored at the spleen and liver. Comparing the number of homed cells to the total number of cells delivered one may postulate the indirect role for few hundred thousands ABMC's at heart regeneration. PMID:17101186

Kurpisz, M; Czepczy?ski, R; Grygielska, B; Majewski, M; Fiszer, D; Jerzykowska, O; Sowi?ski, J; Siminiak, T

2007-10-01

410

Dysplastic Bone Marrow Changes During Maintenance Therapy for Acute Leukemia.  

PubMed

We describe the case of an 8-year-old girl with common precursor B-cell acute lymphoblastic leukemia who presented with severe pancytopenia during maintenance therapy with methotrexate and 6-mercaptopurine. The bone marrow smear showed moderate hypocellularity and trilinear dysplastic changes consistent with a diagnosis of drug toxicity, with no evidence of lymphoblasts. Flow cytometric immunophenotyping was negative for leukemic cells. Blood cell counts normalized after treatment with folinic acid. Maintenance therapy was gradually restarted and she remained well at follow-up visits. Myelotoxicity from methotrexate and 6-mercaptopurine may represent an unpredictable incident during an otherwise uneventful maintenance therapy, and may occur independently of other organ toxicities. PMID:25493456

Chinello, Matteo; Naviglio, Samuele; Shardlow, Alison; Severino, Alessandro; Ventura, Alessandro; Locasciulli, Anna

2014-12-01

411

Protecting the interests of the child bone marrow donor.  

PubMed

At a time when designer babies have been created to act as cord blood donors to sick siblings, ethical debate has focused predominantly on the extent to which it is acceptable to create one human being to assist another. However, children are frequently used this way, by their families and doctors who extract their bone marrow, to try to save the life of another, usually a sibling. With any life-threatening illness, there is the possibility that the urgency of the sick sibling's need means that the short-term welfare of the donor child receives less attention than it should by parents and doctors. This article suggests ways to protect the interests of such children and empower them within the decision-making process and concludes that the drive to save life must be tempered by recognition of the intrinsic worth of donor children and their rights not to be exploited. PMID:15685919

Terry, Louise M; Campbell, Anne

2004-01-01

412

Regenerate augmentation with bone marrow concentrate after traumatic bone loss.  

PubMed

Distraction osteogenesis after post-traumatic segmental bone loss of the tibia is a complex and time-consuming procedure that is often complicated due to prolonged consolidation or complete insufficiency of the regenerate. The aim of this feasibility study was to investigate the potential of bone marrow aspiration concentrate (BMAC) for percutaneous regenerate augmentation to accelerate bony consolidation of the regenerate. Eight patients (age 22-64) with an average posttraumatic bone defect of 82.4 mm and concomitant risk factors (nicotine abuse, soft-tissue defects, obesity and/or circulatory disorders) were treated with a modified Ilizarov external frame using an intramedullary cable transportation system. At the end of the distraction phase, each patient was treated with a percutaneously injection of autologous BMAC into the centre of the regenerate. The concentration factor was analysed using flow cytometry. The mean follow up after frame removal was 10 (4-15) months. With a mean healing index (HI) of 36.9 d/cm, bony consolidation of the regenerate was achieved in all eight cases. The mean concentration factor of the bone marrow aspirate was 4.6 (SD 1.23). No further operations concerning the regenerate were needed and no adverse effects were observed with the BMAC procedure. This procedure can be used for augmentation of the regenerate in cases of segmental bone transport. Further studies with a larger number of patients and control groups are needed to evaluate a possible higher success rate and accelerating effects on regenerate healing. PMID:22577502

Gessmann, Jan; Köller, Manfred; Godry, Holger; Schildhauer, Thomas Armin; Seybold, Dominik

2012-01-01

413

MSC Therapy Attenuates Obliterative Bronchiolitis after Murine Bone Marrow Transplant  

PubMed Central

Rationale Obliterative bronchiolitis (OB) is a significant cause of morbidity and mortality after lung transplant and hematopoietic cell transplant. Mesenchymal stromal cells (MSCs) have been shown to possess immunomodulatory properties in chronic inflammatory disease. Objective Administration of MSCs was evaluated for the ability to ameliorate OB in mice using our established allogeneic bone marrow transplant (BMT) model. Methods Mice were lethally conditioned and received allogeneic bone marrow without (BM) or with spleen cells (BMS), as a source of OB-causing T-cells. Cell therapy was started at 2 weeks post-transplant, or delayed to 4 weeks when mice developed airway injury, defined as increased airway resistance measured by pulmonary function test (PFT). BM-derived MSC or control cells [mouse pulmonary vein endothelial cells (PVECs) or lung fibroblasts (LFs)] were administered. Route of administration [intratracheally (IT) and IV] and frequency (every 1, 2 or 3 weeks) were compared. Mice were evaluated at 3 months post-BMT. Measurements and Main Results No ectopic tissue formation was identified in any mice. When compared to BMS mice receiving control cells or no cells, those receiving MSCs showed improved resistance, compliance and inspiratory capacity. Interim PFT analysis showed no difference in route of administration. Improvements in PFTs were found regardless of dose frequency; but once per week worked best even when administration began late. Mice given MSC also had decreased peribronchiolar inflammation, lower levels of hydroxyproline (collagen) and higher frequencies of macrophages staining for the alternatively activated macrophage (AAM) marker CD206. Conclusions These results warrant study of MSCs as a potential management option for OB in lung transplant and BMT recipients. PMID:25272285

Raza, Kashif; Price, Andrew P.; Meyer, Carolyn; Matson, Amy; Ehrhardt, Michael J.; Fogas, Samuel; Tolar, Jakub; Hertz, Marshall I.; Panoskaltsis-Mortari, Angela

2014-01-01

414

The painful bone marrow edema syndrome of the hip joint.  

PubMed

In this issue of the WKW, Aigner et al have published that, for the first time, a conservative approach with iloprost has shown to be equally successful as the well-documented core decompression surgical approach in patients with BMES of the hip joint. The BME pattern on MR-imaging of the hip joint represents a common but unspecific finding, which may be associated with several diseases requiring different therapeutic strategies (Table 1). It is still controversial, whether BMES of the hip represents a distinct self-limiting disease also known as transient osteoporosis, transient marrow edema, or algodystrophy, or merely reflects a subtype of ON. Since prognosis and therapeutic consequences vary significantly, differential diagnosis between BMES, CRPS and ON is of clinical interest (Table 2). Both, BMES and ON show similar ON risk factors and a male prevalence, while classical CRPS has a history of trauma and a prevalence among females. Clinical presentation of BMES and ON is similar with typical mechanical pain and prevalence of the hip joint. In contrast, classical CRPS shows a diffuse and burning pain in combination with trophic and vasomotor signs, mainly in the hands and feet. Imaging patterns of BMES are more diffuse, across the entire femoral head, while focal and subchondral in ON. In both, the patterns are limited to the femoral head. In contrast to classical CRPS, the imaging changes are located in all periarticular bones, and the soft tissues are always affected. The histological bone marrow changes are similar in all three diseases, but with abundant new bone formation in BMES and CRPS, whereas in ON only limited new bone formation surrounds the focal necrosis with a sclerotic rim. Protected weight-bearing and treatment with iloprost for BMES, but operative treatment for ON, and a sophisticated physiotherapy for CRPS in combination with iloprost are the preferred treatment strategies in our institution. PMID:15847189

Hofmann, Siegfried

2005-02-01

415

Leukemia cells induce changes in human bone marrow stromal cells  

PubMed Central

Background Bone marrow stromal cells (BMSCs) are multipotent cells that support angiogenesis, wound healing, and immunomodulation. In the hematopoietic niche, they nurture hematopoietic cells, leukemia, tumors and metastasis. BMSCs secrete of a wide range of cytokines, growth factors and matrix proteins which contribute to the pro-tumorigenic marrow microenvironment. The inflammatory cytokines IFN-? and TNF-? change the BMSC secretome and we hypothesized that factors produced by tumors or leukemia would also affect the BMSC secretome and investigated the interaction of leukemia cells with BMSCs. Methods BMSCs from healthy subjects were co-cultured with three myeloid leukemia cell lines (TF-1, TF-1? and K562) using a trans-well system. Following co-culture, the BMSCs and leukemia cells were analyzed by global gene expression analysis and culture supernatants were analyzed for protein expression. As a control, CD34+ cells were also cocultured with BMSCs. Results Co-culture induced leukemia cell gene expression changes in stem cell pluripotency, TGF-? signaling and carcinoma signaling pathways. BMSCs co-cultured with leukemia cells up-regulated a number of proinflammatory genes including IL-17 signaling-related genes and IL-8 and CCL2 levels were increased in co-culture supernatants. In contrast, purine metabolism, mTOR signaling and EIF2 signaling pathways genes were up-regulated in BMSCs co-cultured with CD34+ cells. Conclusions BMSCs react to the presence of leukemia cells undergoing changes in the cytokine and chemokine secretion profiles. Thus, BMSCs and leukemia cells both contribute to the creation of a competitive niche more favorable for leukemia stem cells. PMID:24304929

2013-01-01

416

Value of bilateral bone marrow biopsy specimens in non-Hodgkin's lymphoma.  

PubMed Central

A study of 260 patients with non-Hodgkin's lymphoma (NHL) who underwent bilateral bone marrow biopsy at initial diagnosis showed marrow disease in 99 (38%) cases. The highest incidence of disease (83%) was seen in small lymphocytic lymphoma (SLL) and the lowest (19%) in diffuse large cell lymphoma (DLCL). Among cases with positive marrows, disease was bilateral in all 15 cases of SLL but in only 10 of 20 (50%) of the DLCL cases. In 30 of 99 (30%) positive marrows disease was unilateral. Follicular lymphomas were strongly associated with a paratrabecular pattern, with 40 of 45 positive cases showing this. Discordant histology was seen in six of 20 positive cases of DLCL and two of 37 positive cases of follicular small cleaved cell lymphomas (FSCCL). A bone marrow aspirate was positive in only 56 of the 99 (57%) cases. Peripheral blood disease was present in 15% of the bone marrow positive cases and in 6% of the cases overall. The incidence of marrow disease varies with the histological subtype of lymphoma. The paratrabecular pattern is associated with follicular lymphoma, and bilateral biopsy specimens increase the positivity rate in most subtypes of NHL. PMID:2401730

Juneja, S K; Wolf, M M; Cooper, I A

1990-01-01

417

In vivo cell kinetics of the bone marrow transplantation using dual colored transgenic rat system  

NASA Astrophysics Data System (ADS)

Because bone marrow is an adequate site for bone marrow stem cells, intra-bone marrow - bone marrow transplantation (IBM-BMT) is an efficient strategy for bone marrow transplantation (BMT). However, the fate of the transplanted cells remains unclear. Herein, we established a dual-colored transgenic rat system utilizing green fluorescent protein (GFP) and a luciferase (luc) marker. We then utilized this system to investigate the in vivo kinetics of transplanted bone marrow cells (BMCs) after authentic intravenous (IV)-BMT or IBM-BMT. The in vivo fate of the transplanted cells was tracked using an in vivo luminescent imaging technique; alterations in peripheral blood chimerism were also followed using flow cytometry. IBM-BMT and IV-BMT were performed using syngeneic and allogeneic rat combinations. While no difference in the proliferation pattern was observed between the two treatment groups at 7 days after BMT, different distribution patterns were clearly observed during the early phase. In the IBM-BMT-treated rats, the transplanted BMCs were engrafted immediately at the site of the injected bone marrow and expanded more rapidly than in the IV-BMT-treated rats during this phase. Graft-versus-host disease was also visualized. Our bio-imaging system using dual-colored transgenic rats is a powerful tool for performing quantitative and morphological assessments in vivo.

Kai, Kotaro; Teraoka, Satoshi; Adachi, Yasushi; Ikehara, Susumu; Murakami, Takashi; Kobayashi, Eiji

2008-02-01

418

Epstein-Barr virus infection associated with bone marrow fibrin-ring granuloma.  

PubMed

We previously reported 24 cases of marrow fibrin-ring granuloma (FRG) encountered in 1 institution and concluded that, contrary to previous studies showing marrow FRG as a diagnostic marker for Q fever, Epstein-Barr virus (EBV) was the most common proven cause of such FRG. The present study characterized patients with EBV-associated marrow FRG. We retrospectively reviewed 17 cases of EBV-associated FRG (43% of 40 cases with marrow FRG) diagnosed by bone marrow biopsy. Patients with EBV-associated hemophagocytic lymphohistiocytosis (5/17 patients) and chronic active EBV infection (4/17) constituted 53% of patients with EBV-associated FRG. Three patients had lymphoma without marrow involvement. All patients except 1 presented first with fever; splenomegaly was the next most common clinical finding (12/17). Cytopenia was observed in the 17 cases: anemia in 16, thrombocytopenia in 9, and leukopenia in 8. Patients with EBV-associated FRG showed lower survival outcomes than did patients without EBV (median, 3.0 vs 11.8 months; P = .009). Patients with bone marrow FRG accompanied by fever require careful evaluation to discern whether active EBV infection is involved because the prognosis is poor. PMID:20093240

Chung, Hee-Jung; Chi, Hyun-Sook; Jang, Seongsoo; Park, Chan-Jeoung

2010-02-01

419

The possible role of anemia in bone marrow uptake of radiocolloid  

SciTech Connect

Entry of Tc-99m-sulfur colloid into bone marrow is a frequent observation and possible causes have been discussed in the literature. Using a present formulation of the radiocolloid (Medi+Physics), the authors sought to define the distribution patterns of bone marrow uptake, and relate these back to the primary diagnosis. Three hundred consecutive cases (with spleen present) were analyzed. Only 18% of the patients were under age 29 years and 62% were age 50 or older. Posterior images were evaluated so that liver, spleen and bone marrow could be compared visually. Twelve % of the studies were within normal limits. In 33% of the patients, the liver or spleen showed a defect without a radiocolloid shift. In 41% of the cases, there was a shift of radioactivity to the spleen (spleen/liver greater than 1), but not to the bone marrow. In 12% of the patients, there was both a radiocolloid shift to the spleen and visible uptake in the bone marrow. Least common was uptake of radiocolloid in the vertebral bone marrow without a splenic radiocolloid shift: this occurred in only 5 cases (under 2% of the total). Looked at another way the authors can normalize the cases with radiocolloid shift to the spleen (but not elsewhere) to 100%. Then radiocolloid shift to both spleen and bone marrow occurred only 29% as frequently. Radicolloid in the bone marrow, but not shifted to the spleen, was unusual (5% as frequent). These 5 patients were analyzed; all had anemia. The Hb ranged from 7.9 - 10.6 gm/dl, and hematocrits from 23 - 30. Several etiologies were involved. Anemia may be a stimulus to bone marrow uptake of radiocolloid without major splenic accumulation.

Skarzynski, J.J.; Spencer, R.P.; Karlmeddini, M.K.

1984-01-01

420

An Animal Model of Chronic Aplastic Bone Marrow Failure Following Pesticide Exposure in Mice  

PubMed Central

The wide use of pesticides for agriculture, domestic and industrial purposes and evaluation of their subsequent effect is of major concern for public health. Human exposure to these contaminants especially bone marrow with its rapidly renewing cell population is one of the most sensitive tissues to these toxic agents represents a risk for the immune system leading to the onset of different pathologies. In this experimental protocol we have developed a mouse model of pesticide(s) induced hypoplastic/aplastic marrow failure to study quantitative changes in the bone marrow hematopoietic stem cell (BMHSC) population through flowcytometric analysis, defects in the stromal microenvironment through short term adherent cell colony (STACC) forming assay and immune functional capacity of the bone marrow derived cells through cell mediated immune (CMI) parameter study. A time course dependent analysis for consecutive 90 days were performed to monitor the associated changes in the marrow’s physiology after 30th, 60th and 90th days of chronic pesticide exposure. The peripheral blood showed maximum lowering of the blood cell count after 90 days which actually reflected the bone marrow scenario. Severe depression of BMHSC population, immune profile of the bone marrow derived cells and reduction of adherent cell colonies pointed towards an essentially empty and hypoplastic marrow condition that resembled the disease aplastic anemia. The changes were accompanied by splenomegaly and splenic erythroid hyperplasia. In conclusion, this animal model allowed us a better understanding of clinico-biological findings of the disease aplastic anemia following toxic exposure to the pesticide(s) used for agricultural and industrial purposes. PMID:24855541

Chatterjee, Sumanta; Chaklader, Malay; Basak, Pratima; Das, Prosun; Das, Madhurima; Pereira, Jacintha Archana; Dutta, Ranjan Kumar; Chaudhuri, Samaresh; Law, Sujata

2010-01-01

421

Altered canonical hedgehog-gli signalling axis in pesticide-induced bone marrow aplasia mouse model.  

PubMed

The mechanistic interplay between pesticide exposure and development of marrow aplasia is not yet well established but there are indices that chronic pesticide exposure in some instances causes marrow aplasia like haematopoietic degenerative condition in human beings. Canonical Hedgehog (Hh) signalling has multiple roles in a wide range of developmental processes, including haematopoiesis. The present study was designed to explore the status of four important components of the canonical Hedgehog signalling cascade, the Sonic Hedgehog (Shh), Ptch1, Smo, and Gli1, in a mouse model of chronic pesticide-induced bone marrow aplasia. We used 5 % aqueous mixture of pesticides (chlorpyriphos, prophenophos, cypermethrin, alpha-methrin, and hexaconazole) for inhalation and dermal exposure of 6 hours per day and 5 days a week up to 90 days. Murine bone marrow aplasia related to chronic pesticide treatment was confirmed primarily by haemogram, bone marrow cellularity, short term bone marrow explant culture for cellular kinetics, bone marrow smear, and fl ow cytometric Lin-Sca-1+C-kit+ extracellular receptor expression pattern. Later, components of hedgehog signalling were analysed in the bone marrow of both control and pesticide-treated aplastic groups of animals. The results depicted pancytopenic feature of peripheral blood, developmental anomaly of neutrophils, depression of primitive stem and progenitor population along with Shh, Ptch1, Smo and Gli1 expression in aplasia group. This investigation suggests that pesticide-induced downregulation of two critically important proteins--Ptch1 and Gli1--inside the haematopoietic stem and progenitor cell population impairs haematopoietic homeostasis and regeneration mechanism in vivo concurrent with bone marrow aplasia. PMID:23152377

Chaklader, Malay; Das, Prosun; Pereira, Jacintha Archana; Chaudhuri, Samaresh; Law, Sujata

2012-09-01

422

Still from Red Spot Movie  

NASA Technical Reports Server (NTRS)

This image is one of seven from the narrow-angle camera on NASA's Cassini spacecraft assembled as a brief movie of cloud movements on Jupiter. It was taken with a blue filter. The smallest features visible are about 500 kilometers (about 300 miles) across.

Small bright clouds appear suddenly to the west of the Great Red Spot. Based on data from NASA's Galileo spacecraft, scientists suspect that these small white features are lightning storms, where falling raindrops create an electrical charge. The lightning storms eventually merge with the Red Spot and surrounding jets, and may be the main energy source for these large-scale features. Imaging observations of the darkside of the planet in the weeks following Cassini's closest approach to Jupiter on Dec. 30, 2000 will search for lightning storms like these.

This image was re-projected by cylindrical-map projection of an image taken in the first week of October 2000. It shows an area from 50 degrees north of Jupiter's equator to 50 degrees south, extending 100 degrees east west, about one quarter of Jupiter's circumference.

Cassini is a cooperative project of NASA, the European Space Agency and the Italian Space Agency. The Jet Propulsion Laboratory, a division of the California Institute of Technology in Pasadena, manages the Cassini mission for NASA's Office of Space Science, Washington, D.C.

2000-01-01

423

Similar rates of production of T and B lymphocytes in the bone marrow  

PubMed Central

The rate of renewal of T lymphocytes in the bone marrow of euthymic C57BL/Ka and athymic nu/nu BALB/c mice was estimated by in vivo labeling with bromodeoxyuridine. T lymphocytes accounted for 16-18% of marrow cells in euthymic mice as judged by immunofluorescent staining with monoclonal antibodies for Thy-1, CD3, and alpha/beta T cell antigen receptor markers. About 70% of marrow cells expressed receptors (Mac-1, Gr-1, B220) for myeloid, macrophage, and B lineage cells. Approximately 13% of cells in the athymic bone marrow expressed alpha/beta T cell receptors. Sorted marrow T cells proliferated in response to stimulation with anti-alpha/beta antibodies in vitro and showed functional rearrangements of V beta and J beta genes. Sorted non- T cells did not respond to stimulation in vitro, and all V beta and J beta gene rearrangements identified were nonfunctional. In vivo labeling studies indicated that approximately 17 x 10(6) bone marrow T cells are renewed daily in euthymic mice and approximately 14 x 10(6) are renewed in athymic mice. Approximately 11 x 10(6) mature B cells (immunoglobulin M+) are renewed daily in the bone marrow of the latter mice. To determine whether marrow precursors can give rise to T cells directly, marrow cells from euthymic and athymic mice were depleted of T cells by cell sorting and incubated in vitro for 48 h in the absence of exogenous growth factors or thymic stromal cells. Examination of the cells after culture showed that 10-12% stained brightly for alpha/beta T cell receptors. Although functional rearrangements of V beta and J beta genes were not detected before culture, the majority of rearrangements were functional after culture. The emergence of the bright alpha/beta T cells in culture was dependent on depletion T cells from the marrow cells before culture. The results suggest that most marrow T cells are generated in the marrow itself. PMID:7760006

1995-01-01

424

Acute lymphoblastic leukaemia with microfilaria: a rare coincidence in bone marrow aspirate.  

PubMed

Microfilariae in bone marrow aspirates have been reported occasionally, but until now no case has been reported in association with acute leukaemia. This is a unique finding, not mentioned in literature. Microfilariae are seen mainly in peripheral blood smears (PBS) and rarely in bone marrow aspirate, but this surprisingly did not show any parasite in PBS. Meticulous examination of bone marrow smears revealed acute lymphoblastic leukaemia and a few interspersed microfilariae. This rare combination, reported for the first time, definitely needs a space in literature. PMID:22654304

Arundhati; Kumar, Ashok; Kumar, Rakesh

2011-06-01

425

Host bone marrow-derived IL-12 enhances donor T cell engraftment in a mouse model of bone marrow transplantation  

PubMed Central

Background Donor cell engraftment is critical for the success of allogeneic bone marrow transplants. Graft failure is a result of donor cells either failing to engraft initially or being eliminated at later time points. Donor cell engraftment is facilitated by donor T cells, which eliminate residual host hemato-lymphoid effector cells such as NK cells and T cells. Methods We aimed to explore the role of host hematopoietic cell derived IL-12 on donor cell engraftment in a murine model of BMT. We established radiation chimeras by transplanting C57BL6/J (B6) mice with BM from either congenic B6 mice or IL-12p40 KO mice. These WT ? WT or IL-12 KO ? WT chimeras then underwent a secondary transplant with allogeneic (FVB) BM. Survival, engraftment, donor T cell expansion, cytokine production by donor T cells, as well as expression of stimulatory markers on donor T cells was analyzed. Results Mice whose residual host hematopoietic cells were capable of producing IL-12 had modestly higher survival, higher donor T cell engraftment, and significantly higher donor erythroid engraftment. We have also found that an increased number of donor T cells in IL-12 KO ? WT chimeras have a regulatory phenotype, expressing FoxP3, producing lower levels of TNF-?, higher levels of IL-10, and expressing higher levels of ICOS as well as PD-1 on CD4+ T cells. Conclusions To our knowledge, this is the first report of a beneficial role of IL-12 production by host cells in the context of bone marrow engraftment in a murine model of BMT. These findings support the clinical use of exogenous IL-12 for use in settings where graft failure is of concern. PMID:24580829

2014-01-01

426

Improved Prediction of Myelotoxicity Using a Patient-Specific Imaging Dose Estimate for Non-Marrow-Targeting90Y-Antibody Therapy  

Microsoft Academic Search

For calculation of radiation dose to the marrow, standard do- simetry for radiopharmaceuticals that do not bind to the marrow includes dose contributions from radioactivity in blood and the remainder of the body. For a pure -emitter such as 90Y, marrow dose is usually determined by the blood contribution. However, myelotoxicity from 90Y-antibody therapy often correlates poorly with marrow dose

Sui Shen; Ruby F. Meredith; Jun Duan; Daniel J. Macey; M. B. Khazaeli; Francisco Robert; Albert F. LoBuglio

427

Panax notoginseng saponins promote osteogenic differentiation of bone marrow stromal cells through the ERK and P38 MAPK signaling pathways.  

PubMed

The Chinese medicinal herb, Panax notoginseng, has long been used to treat bone fractures and Panax notoginseng saponins (PNS) could promote bone formation. Here, we investigated whether PNS could promote osteogenesis of bone marrow stromal cells (BMSCs) through modulating the MAPK signaling pathways, which are implicated in BMSC osteogenesis. We found that PNS markedly increased the mineralization of BMSCs by alizarin red S assays and stimulate alkaline phosphatase activity of these cells. Additionally, PNS significantly increased the mRNA levels of alkaline phosphatase, core-binding factor a1, and bone sialoprotein while decreasing PPAR?2 mRNA levels. Furthermore, inhibitors of ERK, PD98059, and p38, SB203580 inhibited the osteogenesis-potentiating effects by PNS. PNS stimulated the activation of ERK and p38 as evidenced by increased phosphorylation of these proteins, which was inhibited by PD98059 and SB203580. Our findings indicate that PNS could promote BMSC osteogenesis by activating the ERK and p38 signaling pathways. PMID:21865745

Li, Xue-Dong; Liu, Zhao-Yong; Chang, Bo; Liu, Dong-Xin; Chen, Bin; Guo, Chun; Wang, Yun-Guo; Xu, Jian-Kun; Huang, Dong-Yang; Du, Shi-Xin

2011-01-01

428

Curcumin-functionalized silk materials for enhancing adipogenic differentiation of bone marrow-derived human mesenchymal stem cells.  

PubMed

Curcumin, a natural phenolic compound derived from the plant Curcuma longa, was physically entrapped and stabilized in silk hydrogel films, and its influence on human bone marrow-derived mesenchymal stem cells (hBMSC) was assessed related to adipogenic differentiation. The presence of curcumin significantly reduced the silk gelation time and changed the porous morphology of gel matrix, but did not change the formation of the silk beta-sheet structure. Based on spectrofluorimetric analysis, curcumin most likely interacted with hydrophobic residues in silk, interacting with the beta-sheet domains formed in the hydrogels. The antioxidant activity of silk film-associated curcumin remained functional over at least one month in both the dry and hydrated state. Negligible curcumin was released from silk hydrogel films over 48h incubation in aqueous solution. For hBMSC cultured on silk films containing more than 0.25mgml(-1) curcumin, cell proliferation was inhibited, while adipogenesis was significantly promoted based on transcripts as well as Oil Red O staining. When hBMSC were cultured in media containing free curcumin, both proliferation and adipogenesis of hBMSC were inhibited when curcumin concentrations exceeded 5?M, which is more than 1000times higher than the level of curcumin released from the films in aqueous solution. Thus, silk film-associated curcumin exhibited different effects on hBMSC proliferation and differentiation compared with curcumin in solution. PMID:25132274

Li, Chunmei; Luo, Tingting; Zheng, Zhaozhu; Murphy, Amanda R; Wang, Xiaoqin; Kaplan, David L

2015-01-01

429

The effect of a red leaf pigment on the relationship between red edge and chlorophyll concentration  

NASA Technical Reports Server (NTRS)

The effect of a leaf pigment - red amaranthin - on red edge and chlorophyll concentration is investigated in amaranth leaves by means of treatments with nitrate and salts. A near-linear relationship between red edge and chlorophyll concentration is observed for leaves with low amaranthin concentration, and no relationship is noted at high concentrations. The study demonstrates the limitation inherent in estimating chlorophyll concentration by using remotely sensed red edge.

Curran, Paul J.; Dungan, Jennifer L.; Macler, Bruce A.; Plummer, Stephen E.

1991-01-01

430

Red blood cell malformations Cell shapes Modeling and simulation of red blood cell light scattering  

E-print Network

Red blood cell malformations Cell shapes Modeling and simulation of red blood cell light to various diseases and acute conditions, the shape and composition of erythrocytes (red blood cells. To the right is a figure depicting the initial stages of a beam traversal through a sample of blood cells

California at Berkeley, University of

431

Voyager 1 Red Spot Movie  

NASA Technical Reports Server (NTRS)

This movie shows the portion of Jupiter around the Great Red Spot as it swirls through more than 60 Jupiter days. Notice the difference in speed and direction of the various zones of the atmosphere. The interaction of the atmospheric clouds and storm shows how dynamic the Jovian atmosphere is.

As Voyager 1 approached Jupiter in 1979, it took images of the planet at regular intervals. This sequence is made from 66 images taken once every Jupiter rotation period (about 10 hours). This time-lapse movie uses images taken every time Jupiter longitude 68W passed under the spacecraft. These images were acquired in the Blue filter from Jan. 6 to Feb. 3 1979. The spacecraft flew from 58 million kilometers to 31 million kilometers from Jupiter during that time.

This time-lapse movie was produced at JPL by the Image Processing Laboratory in 1979.

2000-01-01

432

The Piromen Test as an Assay of Bone Marrow Granulocyte Reserves in the Calf I. Studies on Bone Marrow and Peripheral Blood Leukocytes  

PubMed Central

The use of Piromen, a polysaccharide complex of Pseudomonas aeroginosa, has been investigated in 21 calves by a test of marrow granulocyte reserves. The maximal increase in peripheral granulocytes (?G) was determined following multiple intravenous and sub-cutaneous injections of Piromen at various time intervals and was correlated with the mature marrow granulocytes on bone marrow smear differentials. Five ug Piromen per kg body weight, by the subcutaneous route, was found to give a mean ?G of 5200/mm3, very similar to the ?G of 5300/mm3 obtained in man with 0.1 ug/kg intravenous Piromen injections. Clinical effects in calves were minimal with the subcutaneous route as compared to the response following intravenous Piromen injections. PMID:4272958

Lumsden, J. H.; Valli, V. E. O.; McSherry, B. J.; Willoughby, R. A.

1974-01-01

433

Celebrating 40 years of progress in bone marrow transplantation: a report from the 40th Annual Meeting of the European Society for Blood and Marrow Transplantation.  

PubMed

The European Society for Blood and Marrow Transplantation was established in 1974 to enable scientists and physicians involved in clinical bone marrow transplantation to share their experience and develop cooperative studies. The organization celebrated its 40th anniversary with a meeting that considered hematopoietic stem cell transplantation not as a standalone procedure, but as part of a complex therapeutic program managed by a multidisciplinary professional team. The role of antifungal prophylaxis, emerging resistance in Aspergillus, the management of mucormycosis and new guidelines on antifungal therapy were among the topics discussed by the physicians, nurses, allied health professionals and scientists attending the 40th Annual Meeting of the European Society for Blood and Marrow Transplantation. PMID:25405881

Pagano, Livio; Lyon, Sue

2014-01-01

434

Sideroblastic changes of the bone marrow can be predicted by the erythrogram of peripheral blood  

PubMed Central

The diagnosis of sideroblastic anemia is based on bone marrow aspiration, and the detection of ring sideroblasts (RS) in iron staining. The finding of laboratory parameters to approach this diagnosis still remains a great challenge. In this study, we analyzed the value of a specific erythrogram pattern from peripheral blood, produced by the ADVIA®120 cell counter, to predict sideroblastic changes in the bone marrow. In a two step-design study, we first showed that 32/38 consecutive patients reporting ?15% RS had such a pattern in the erythrogram. In the second step, we prospectively identified over a period of 32 months 21 patients with this typical erythrogram; 20/21 had ?15% RS in the bone marrow. Hence, by this validation, we confirm that the erythrogram is highly predictive of RS in the bone marrow. The interpretation of the erythrogram should become daily practice in hematology to improve the efficacy to detect sideroblastic changes. PMID:19706066

ROVÓ, A; STÜSSI, G; MEYER-MONARD, S; FAVRE, G; TSAKIRIS, D; HEIM, D; HALTER, J; ARBER, C; PASSWEG, J; GRATWOHL, A; TICHELLI, A

2010-01-01

435

Study of Cancer Susceptibility Among Patients with Inherited Bone Marrow Failure Syndromes  

Cancer.gov

Persons with inherited bone marrow failure syndromes (IBMFS) tend to have a very high risk of developing certain cancers. Researchers are assembling a study population that includes IBMFS patients and their family members.

436

What to Expect Before a Blood and Marrow Stem Cell Transplant  

MedlinePLUS

... Expect Before a Blood and Marrow Stem Cell Transplant Finding a Donor If you're going to ... body. HLA Tissue Typing People having stem cell transplants are matched with donors through a test called ...

437

NCI-CCR Pediatric Oncology Branch: Blood and Marrow Transplant - Staff  

Cancer.gov

Skip to Main Content CCR Home | About CCR | CCR Intranet Main Navigation Referrals For Patients For Physicians For Prospective Trainees For Scientists News Quick Links Home Referring a Patient Patients and Families Scientific Programs - Blood & Marrow

438

Isolation of (+)-catechin and (-)-epicatechin from Actinidia arguta as bone marrow cell proliferation promoting compounds.  

PubMed

The MeOH extract of stems of Actinidia arguta promoted proliferation of cultured bone marrow cells and stimulated formation of myeloid colonies from bone marrow cells. (+)-Catechin ( 1) and (-)-epicatechin ( 2) were isolated as active compounds from the MeOH extract. Compounds 1 and 2 stimulated the cell proliferation in a concentration-dependent manner in the range of 1 to 100 mg/mL. Compounds 1 and 2 also stimulated formation of myeloid colonies and enhanced the effect of interleukin-3 (IL-3) to increase the number of colony forming-units in culture (CFU-c). In an ex vivo experiment using a model mouse of decreasing bone marrow functions, orally administrated 1 (100 mg/kg/day) stimulated IL-3-induced CFU-c formation of the bone marrow cells. PMID:12709898

Takano, Fumihide; Tanaka, Tomoaki; Tsukamoto, Eiko; Yahagi, Nobuo; Fushiya, Shinji

2003-04-01

439

Discovery of novel anti-inflammatory proteins inspired by bone marrow mesenchymal stem cell secretions  

E-print Network

Bone marrow mesenchymal stem cells (MSCs) may soon become the first FDA-approved stem cell therapy for autoimmune and inflammatory disease. Our lab originally hypothesized that much of the therapeutic activity of MSCs may ...

Milwid, Jack Miles

2011-01-01

440

Granulopoiesis in infantile genetic agranulocytosis. In vitro cloning of marrow cells in agar culture.  

PubMed

A 3-year-old boy and a 2-year-old girl with infantile genetic agranulocytosis have been studied by in vitro cloning of bone marrow cells in agar culture. The patients display a normal concentration of colony forming cells and the morphological maturation is identical with that of control marrow cultured in vitro. The marrow cells of the patients show some degree of auto-stimulation indicating that endogenous production of colony stimulating factor is operating. As an inverse relationship is expected between the peripheral neutrophil count and the percentage of marrow colony forming cells in S-phase a high percentage was expected. On the contrary, we find that the percentage of colony forming cells in S-phase is extremely low indicating a genetic unresponsiveness of granulopoietic precursor cells to feed back regulation in infantile genetic agranulocytosis. PMID:1082629

Olofsson, T; Olsson, I; Kostmann, R; Malmström, S; Thilen, A

1976-01-01

441

Pulmonary infections in the late period after allogeneic bone marrow transplantation: chest radiography versus computed tomography  

Microsoft Academic Search

Purpose:To analyze the capabilities of chest roentgenogram (CXR) and computed tomography (CT) in the evaluation of pulmonary infectious disease in the late period (>100 days) after allogeneic bone marrow transplantation (BMT).

Gerd Schueller; Wolfgang Matzek; Peter Kalhs; Cornelia Schaefer-Prokop

2005-01-01

442

Stem Cell Transplant (Peripheral Blood, Bone Marrow, and Cord Blood Transplants)  

MedlinePLUS

... cell can form more blood cells than a stem cell from adult bone marrow. Still, cord blood transplants can take ... This takes a lot of time and emotional energy from the patient, caregivers, and loved ones. It’s ...

443

Melnick-Neddles osteodysplasia associated with pulmonary hypertension, obstructive uropathy and marrow hypoplasia  

Microsoft Academic Search

A three and one half-year-old female with Melnick-Needles osteodysplasia is presented. In addition to the characteristic bone roentgenograms and obstructive uropathy, the patient had inadequate formation of bone marrow elements and pulmonary artery hypertension.

R. B. Klint; M. H. Agustsson; W. H. McAlister

1977-01-01

444

Rectal cancer with disseminated carcinomatosis of the bone marrow: report of a case.  

PubMed

Abstract We report a rare case of disseminated carcinomatosis of the bone marrow from rectal cancer with disseminated intravascular coagulation (DIC). A 65-year-old man was admitted with melena and low back pain at rest. X-ray examination showed rectal cancer with multiple bone metastases. Laboratory examination showed severe anemia and DIC. Histologic examination showed disseminated carcinomatosis of the bone marrow. The DIC was considered to be caused by disseminated carcinomatosis of the bone marrow from rectal cancer, and we immediately started treatment with anti-DIC therapy and anticancer chemotherapy with the modified FOLFOX6 regimen (mFOLFOX6). After some response to therapy, the patient's general condition deteriorated, and he died 128 days after admission. This is the first English report showing disseminated carcinomatosis of the bone marrow from colorectal cancer treated with mFOLFOX6. PMID:25216414

Nakashima, Yuichiro; Takeishi, Kazuki; Guntani, Atsushi; Tsujita, Eiji; Yoshinaga, Keiji; Matsuyama, Ayumi; Hamatake, Motoharu; Maeda, Takashi; Tsutsui, Shinichi; Matsuda, Hiroyuki; Fujihara, Megumu; Ishida, Teruyoshi

2014-01-01

445

Bone marrow fat: linking adipocyte-induced inflammation with skeletal metastases  

PubMed Central

Adipocytes are important but underappreciated components of bone marrow microenvironment, and their numbers greatly increase with age, obesity, and associated metabolic pathologies. Age and obesity are also significant risk factors for development of metastatic prostate cancer. Adipocytes are metabolically active cells that secrete adipokines, growth factors, and inflammatory mediators; influence behavior and function of neighboring cells; and have a potential to disturb local milleu and dysregulate normal bone homeostasis. Increased marrow adiposity has been linked to bone marrow inflammation and osteoporosis of the bone, but its effects on growth and progression of prostate tumors that have metastasized to the skeleton are currently not known. This review focuses on fat-bone relationship in a context of normal bone homeostasis and metastatic tumor growth in bone. We discuss effects of marrow fat cells on bone metabolism, hematopoiesis, and inflammation. Special attention is given to CCL2- and COX-2-driven pathways and their potential as therapeutic targets for bone metastatic disease. PMID:24398857

Hardaway, Aimalie L.; Herroon, Mackenzie K.; Rajagurubandara, Erandi

2014-01-01

446

Total marrow irradiation using Helical TomoTherapy  

NASA Astrophysics Data System (ADS)

Clinical dose response data of human tumours are limited or restricted to a radiation dose range determined by the level of toxicity to the normal tissues. This is the case for the most common disseminated plasma cell neoplasm, multiple myeloma, where the maximum dose deliverable to the entire bony skeleton using a standard total body irradiation (TBI) technique is limited to about 12 Gy. This study is part of scientific background of a phase I/II dose escalation clinical trial for multiple myeloma using image-guided intensity modulated radiotherapy (IG-IMRT) to deliver high dose to the entire volume of bone marrow with Helical TomoTherapy (HT). This relatively new technology can deliver highly conformal dose distributions to complex target shapes while reducing the dose to critical normal tissues. In this study tools for comparing and predicting the effectiveness of different approaches to total marrow irradiation (TMI) using HT were provided. The expected dose response for plasma cell neoplasms was computed and a radiobiological evaluation of different treatment cohorts in a dose escalating study was performed. Normal tissue complication probability (NTCP) and tumour control probability (TCP) models were applied to an actual TMI treatment plan for a patient and the implications of using different longitudinal field widths were assessed. The optimum dose was ˜39 Gy for which a predicted tumour control of 95% (+/-3%) was obtained, with a predicted 3% (0, 8%) occurrence of radiation pneumonitis. Tissue sparing was seen by using smaller field widths only in the organs of the head. This suggests it would be beneficial to use the small fields in the head only since using small fields for the whole treatment would lead to long treatment times. In TMI it may be necessary to junction two longitudinally adjacent treatment volumes to form a contiguous planning target volume PTV. For instance, this is the case when a different SUP-INF spatial resolution is required or when the PTV length exceeds the bed travel distance. In this work, the dosimetric challenges associated with junctioning longitudinally adjacent PTVs with HT were analyzed and the feasibility of PTV junctioning was demonstrated. The benefits of spatially dividing or splitting the treatment into a few sub-treatments along the longitudinal direction were also investigated.

Garcia-Fernandez, Lourdes Maria

447

Lentiviral tracking of vascular differentiation in bone marrow progenitor cells.  

PubMed

Lentiviral vectors encoding for identifiable marker genes controlled by lineage-specific promoters can be used to track differentiation of bone marrow progenitors into endothelial cells and/or smooth muscle cells. Human VE-Cadherin and Smoothelin-B promoters were cloned into a self-inactivating lentiviral vector (HR-VECad and HR-SMTHB) and used to drive expression of green fluorescent protein (eGFP). These constructs demonstrated specific promoter activity in mature endothelial and smooth muscle cells respectively in vitro. Lin(-) bone marrow progenitor cells (Lin(-) BMCs) in culture were used to test vector ability to track vascular differentiation. HR-VECad transduced Lin(-) BMCs were plated on collagen-coated slides and grown in endothelial media, while HR-SMTHB transduced Lin(-) BMCs were cultured on fibronectin-coated slides and grown in smooth muscle media. For in vivo differentiation assessment, lentiviral transduced Lin(-) BMCs resuspended in Matrigel were injected subcutaneously into C57BL/6J mice. Explants were evaluated for eGFP expression. Lin(-) BMCs grown in endothelial differentiation media produced groups of polygonal endothelial-like cells by days 16-21. When transduced with HR-VECad vector, these expressed eGFP in distinct cells within the colony by days 18-21, and coexpressed VE-Cadherin and eNOS. Lin(-) BMCs grown in smooth muscle differentiation media produced spindle-shaped cells between days 10-14 in culture. When transduced with the HR-SMTHB vector, these cells showed eGFP expression at approximately 12 days, which increased over time and coexpressed alphaSMA, calponin and myosin heavy chain. Within Matrigel plugs containing HR-VECad transduced cells, eGFP(+) constituted 0.4+/-0.2% of total cells. In contrast, within Matrigel plugs containing HR-SMTHB transduced cells, eGFP(+) cells constituted 0.2+/-0.1% of total cells. These data demonstrate the feasibility of selectively marking BMC populations for cell fate determination. PMID:19715661

Schmeckpeper, Jeffrey; Ikeda, Yasuhiro; Kumar, Arun H S; Metharom, Pat; Russell, Stephen J; Caplice, Noel M

2009-01-01

448

Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes  

Microsoft Academic Search

Recent studies have suggested that bone marrow cells possess a broad differentiation potential, being able to form new liver cells, cardiomyocytes and neurons. Several groups have attributed this apparent plasticity to `transdifferentiation'. Others, however, have suggested that cell fusion could explain these results. Using a simple method based on Cre\\/lox recombination to detect cell fusion events, we demonstrate that bone-marrow-derived

Manuel Alvarez-Dolado; Ricardo Pardal; Jose M. Garcia-Verdugo; John R. Fike; Hyun O. Lee; Klaus Pfeffer; Carlos Lois; Sean J. Morrison; Arturo Alvarez-Buylla

2003-01-01

449

Cell fusion is the principal source of bone-marrow-derived hepatocytes  

Microsoft Academic Search

Evidence suggests that haematopoietic stem cells might have unexpected developmental plasticity, highlighting therapeutic potential. For example, bone-marrow-derived hepatocytes can repopulate the liver of mice with fumarylacetoacetate hydrolase deficiency and correct their liver disease. To determine the underlying mechanism in this murine model, we performed serial transplantation of bone-marrow-derived hepatocytes. Here we show by Southern blot analysis that the repopulating hepatocytes

Xin Wang; Holger Willenbring; Yassmine Akkari; Yumi Torimaru; Mark Foster; Muhsen Al-Dhalimy; Eric Lagasse; Milton Finegold; Susan Olson; Markus Grompe

2003-01-01

450

Perivascular clusters of dendritic cells provide critical survival signals to B cells in bone marrow niches  

Microsoft Academic Search

Beyond its established function in hematopoiesis, the bone marrow hosts mature lymphocytes and acts as a secondary lymphoid organ in the initiation of T cell and B cell responses. Here we report the characterization of bone marrow–resident dendritic cells (bmDCs). Multiphoton imaging showed that bmDCs were organized into perivascular clusters that enveloped blood vessels and were seeded with mature B

Anita Sapoznikov; Yael Pewzner-Jung; Vyacheslav Kalchenko; Rita Krauthgamer; Idit Shachar; Steffen Jung

2008-01-01

451

Effect of interleukin 3 on the differentiation and histamine content of cultured bone marrow mast cells  

Microsoft Academic Search

Mouse bone marrow hematopoietic stem cells were isolated from mouse femur bone and cultured in RPMI 1640 supplemented medium with 20 units\\/ml of the purified T-cell lymphokine, interleukin 3 (IL-3). IL-3 was uniquely able to induce the proliferation and differentiation of mature mast cellsin vitro. The sparse granulation of the bone marrow-derived mast cells (BMMC) can be seen by day

H. F. Chiu; B. A. Burrall

1990-01-01

452

Microenvironmental niches in the bone marrow required for B-cell development  

Microsoft Academic Search

B-cell development is known to occur in a complex bone-marrow microenvironment but its functional organization remains unclear. It is thought that bone-marrow stromal cells create distinct microenvironments, known as niches, that provide support for haematopoiesis and B-cell development. Although it has been more than 20 years since the development of a culture system that allows the growth of B-cell progenitors

Takashi Nagasawa

2006-01-01

453

A Prospective Study of Androgens and Bone Marrow Transplantation for Treatment of Severe Aplastic Anemia  

Microsoft Academic Search

One hundred ten patients with severe aplastic anemia were entered into a prospective study. Forty-seven patients who had HIA-identical siblings were treated with marrow transplantation. Sixty-three patients without marrow don- ors were randomized to supportive care with oral androgen (27 patients). intramus- cular androgen (23 patients). or no androgen (1 3 patients). The distributions of the survival times for the

Bruce M. Camitta; E. Donnall Thomas; David G. Nathan; Robert P. Gale; Kenneth J. Kopecky; Joel M. Rappeport; George Santos; E. C. Gordon-Smith; Rainer Storb

1979-01-01

454

Stromal damage as consequence of high-dose chemo\\/radiotherapy in bone marrow transplant recipients  

Microsoft Academic Search

Bone marrow transplant (BMT) relies on the engraftment of donor hemopoietic precursors in the host marrow space. Colony forming units-fibroblasts (CFU-f), the precursor compartment for the osteogenic lineage, are essential to hemopoietic stem cell survival, proliferation and differentiation. We have studied CFU-f in donors (aged 5 months to 62 years) and in patients who had received allogeneic BMT (aged 2

Maria Galotto; Giovanni Berisso; Laura Delfino; Marina Podesta; Laura Ottaggio; Sandro Dallorso; Carlo Dufour; Giovanni Battista Ferrara; Angelo Abbondandolo; Giorgio Dini; Andrea Bacigalupo; Ranieri Cancedda; Rodolfo Quarto

1999-01-01

455

Lesions of bone and bone marrow in myeloid leukosis occurring naturally in adult broiler breeders.  

PubMed

Lesions of bone and bone marrow in myeloid leukosis (ML) occurring naturally in adult broiler breeders were investigated pathologically. During gross examination, nodules and protrusions were commonly observed on the surface of the sternum, ribs, vertebrae, and synsacrum. The bone marrow of all the bones of the body was pale in color. Histologically, granulated myelocytes proliferated in the bone marrow of various bones and in the periosteum of the sternum, ribs, vertebrae, and synsacrum. The first proliferation of tumor cells occurred in the bone marrow of epiphysis. The myelocytes invaded through haversian and Volkmann's canals from the bone marrow to periosteal areas. Hematopoiesis was suppressed by marked proliferation of tumor cells in the bone marrow of the whole bone. Atrophy was also seen in the bones, including medullary bones of the chickens suffering from ML. Proliferation of myelocytes was seen in the bone marrow and periosteum of ossified cartilaginous rings of the trachea and larynx. Marked proliferation of myelocytes was seen in the dura mater of spinal cords, and it subsequently depressed the spinal cords. Bone formation with cartilage was seen in the periosteum of the sternum having marked proliferation of myelocytes in the bone marrow and periosteum. Ultrastructurally, tumor cells showed large nuclei and cytoplasm with large round electron-dense lysosomes. The virus particles were rarely detected in the cytoplasm of tumor cells. The polymerase chain reaction test of tumor samples showed positive for subgroup J avian leukosis virus. This study indicates that the myelocytes can invade through the compact bones to the periosteum in the sternum, ribs, vertebrae, synsarcum, and ossified cartilage of trachea and larynx having thinner compact bones. In addition, the periosteal osteogenesis with cartilage in the sternum may be reactive change against the bone atrophy because of the marked proliferation of myelocytes. PMID:10737666

Nakamura, K; Ogiso, M; Tsukamoto, K; Hamazaki, N; Hihara, H; Yuasa, N

2000-01-01

456

Long-term culture of leukemic bone marrow primary cells in biomimetic osteoblast niche  

Microsoft Academic Search

We constructed a “biomimetic osteoblast niche” with bio-derived bone as a scaffold, on which we seeded marrow mesenchymal\\u000a stem cells (MSCs) from CML patients, and induced the MSCs to differentiate into osteoblasts. Bone marrow mononuclear cells\\u000a from CML patients were cultured in the biomimetic niche (3D culture system) or a 2D culture system with the induced MSCs\\/osteoblasts\\u000a as a feeder

Li Hou; Ting Liu; Jing Tan; Wentong Meng; Li Deng; Hongtao Yu; Xingli Zou; Yuchun Wang

2009-01-01

457

Matrix metalloproteinase regulation of sphingosine-1-phosphate-induced angiogenic properties of bone marrow stromal cells  

Microsoft Academic Search

Objective. Bone marrow–derived stromal cells (MSC) are able to acquire histological and immunophenotypic characteristics consistent with endothelial cells (EC). In this study we examined the effect of sphingosine-1-phosphate (S1P), a platelet-derived bioactive lysophospholipid that is believed to specifically stimulate EC migration and tube formation, on the angiogenic properties of MSC.Methods. MSC were isolated from murine bone marrow and cultured in

Borhane Annabi; Sébastien Thibeault; Ying-Ta Lee; Nathalie Bousquet-Gagnon; Nicoletta Eliopoulos; Stéphane Barrette; Jacques Galipeau; Richard Béliveau

2003-01-01

458

THE GROWTH OF CHICKEN BONE MARROW IN VITRO AND ITS BEARING ON HEMATOGENESIS IN ADULT LIFE  

PubMed Central

1. The development of the polymorphonuclear leucocyte from a mesenchymal, lymphoid type of cell is possible in adult life and in vitro in chicken bone marrow. 2. These cells can rapidly proliferate by means of amitotic division of an unripe form very similar to, but smaller than themselves. 3. All the cells of chicken bone marrow can undergo a transformation to a type of cell resembling, although not proved to be identical with the cells of connective tissue. PMID:19867624

Foot, Nathan Chandler

1913-01-01

459

CD19 and immunophenotype of bone marrow plasma cells in monoclonal gammopathy of undetermined significance  

Microsoft Academic Search

AIMS--To determine whether a particular phenotype or antigen is preferentially related to monoclonal gammopathies of undetermined significance (MGUS). METHODS--Bone marrow specimens from 56 patients with MGUS were stained immunocytochemically (ABC peroxidase) for CD38, CD56, CD9, CD10, CD19, CD20, CD22, and MB2. Specimens from patients recently diagnosed with multiple myeloma and reactive bone marrow samples were studied in parallel. RESULTS--CD38 was

M Zandecki; T Facon; F Bernardi; V Izydorczyk; L Dupond; M François; R Reade; T Iaru; F Bauters; A Cosson

1995-01-01

460

Bone Marrow Support of the Heart in Pressure Overload Is Lost with Aging  

Microsoft Academic Search

RationaleExogenous stem cell delivery is under investigation to prevent and treat cardiac dysfunction. It is less studied as to the extent endogenous bone marrow derived stem cells contribute to cardiac homeostais in response to stress and the affects of aging on this stress response.ObjectiveTo determine the role of bone marrow (BM) derived stem cells on cardiac homeostasis in response to

Nikolai A. Sopko; Benjamin A. Turturice; Mitchell E. Becker; Chase R. Brown; Feng Dong; Zoran B. Popovic; Marc S. Penn; Felipe Prosper

2010-01-01

461

Perfusion Enhances Functions of Bone Marrow Stromal Cells in Three-Dimensional Culture  

Microsoft Academic Search

Perfusion of medium through three-dimensional (3D) collagen sponges enhanced viability and function of cocultivated marrow stromal and hematopoietic cell lines. Cells of the murine bone marrow stromal cell line GPIa were cultured in novel 3D collagen sponges, made from pepsin-digested bovine skin. Static cultures of sponges were maintained in dishes with media changes every other day. Perfused sponges were contained

Julie Glowacki; Shuichi Mizuno; Joel S Greenberger

1998-01-01

462

Evidence for Structured Variation in Self-Renewal Capacity within Long-Term Bone Marrow Cultures  

Microsoft Academic Search

Bone marrow pluripotent stem cells (CFUs) demonstrate capacity for both proliferation and differentiation. The proliferative capacity of CFUs has been measured by serial transplantability and by the Rs, a measurement of CFU production in a single 14-day transfer. In the present study, the self-renewal capacity of both adherent and nonadherent CFUs from long-term bone marrow cultures was measured. Culture conditions

Peter Mauch; Joel S. Greenberger; Leslie Botnick; Eileen Hannon; Samuel Hellman

1980-01-01

463

Functional tissue-engineered blood vessels from bone marrow progenitor cells  

Microsoft Academic Search

Objective: Stem cells have significant potential for development of cell-based therapeutics for cardiovascular tissue regeneration. Methods: We developed a novel method for isolating smooth muscle cells (SMC) from ovine bone marrow using a tissue-specific promoter and fluorescence-activated cell sorting. Results: As compared to vascular SMC, bone marrow-derived smooth muscle progenitor cells (BM-SMPC) exhibited similar morphology, showed higher proliferation potential and

Jin Yu Liu; Daniel D. Swartz; Hao Fan Peng; Sylvia F. Gugino; James A. Russell; Stelios T. Andreadis

2007-01-01

464

Transformation potential of bone marrow stromal cells into undifferentiated high-grade pleomorphic sarcoma  

Microsoft Academic Search

Purpose  Bone marrow adherent cells contain conventional bone marrow stromal cells and mesenchymal stem cells and these cells constitute\\u000a the hematopoietic microenvironment. Mesenchymal stem cells have the capacity to give rise to multiple mesenchymal lineage\\u000a cells and even ectodermal lineage cells. In the present study, we investigated what types of tumor cells are inducible from\\u000a BM adherent cells by chemical carcinogens.

Qing Li; Hiroko Hisha; Takashi Takaki; Yasushi Adachi; Ming Li; Changye Song; Wei Feng; Satoshi Okazaki; Tomomi Mizokami; Junko Kato; Muneo Inaba; Naoki Hosaka; Masahiko Maki; Susumu Ikehara

2010-01-01

465

Molecular relapse in chronic myelogenous leukemia patients after bone marrow transplantation detected by polymerase chain reaction  

Microsoft Academic Search

Relapse of chronic myelogenous leukemia after bone marrow transplantation can be detected by using clinical, cytogenetic, or molecular tools. A modification of the polymerase chain reaction can be used in patients to detect low levels of the BCR-ABL-encoded mRNA transcript, a specific marker for chronic myelogenous leukemia. Early detection of relapse after bone marrow transplantation could potentially alter treatment decisions.

C. L. Sawyers; L. Timson; S. S. Clark; O. N. Witte; R. Champlin; E. S. Kawasaki

1990-01-01

466

A blinded study of bone marrow examinations in patients with primary immune thrombocytopenia  

PubMed Central

Objective The role of bone marrow examinations in patients with primary immune thrombocytopenia (ITP) is uncertain. The objectives of this study were to determine the inter-rater reliability of bone marrow examinations and to identify distinguishing morphological features of ITP bone marrows under controlled conditions. Methods Histological slides of bone marrow biopsy specimens and aspirates from 32 adult patients with severe primary ITP who had failed a median of two treatments, and 51 non-thrombocytopenic controls were retrieved from hospital archives. Slides were arranged in random order in a slide box and coded. Blinded to the diagnosis and platelet counts, three independent hematopathologists were asked to identify the ITP bone marrows and to evaluate megakaryocyte number, morphology, and distribution. Results Overall chance-corrected agreement on ITP classification among the three raters was poor [kappa (?) = 0.30; 95% confidence interval 0.22–0.38]. Raters were generally unable to correctly identify the ITP bone marrows from controls. Increased number of megakaryocytes, while an uncommon finding, was more frequent among ITP patients compared with controls (6/32, 18.8%; vs. 2/51, 3.9%; P = 0.05), and abnormal megakaryocyte morphology often led individual raters to reach a diagnosis of ITP. Overall sensitivity and specificity of bone marrow examinations were 24% and 90%, respectively. Conclusions This study confirms methodologically that bone marrow examinations are unreliable and frequently non-diagnostic in ITP. Thus, they are not useful for patients with typical disease. Rare subsets of patients with severe ITP demonstrated unique features such as increased number of megakaryocytes. PMID:23140198

Mahabir, Vishwanath K.; Ross, Catherine; Popovic, Snezana; Sur, Mona Lisa; Bourgeois, Jacqueline; Lim, Wendy; George, James N.; Wang, Grace; Cook, Richard J.; Toltl, Lisa J.; Nazi, Ishac; Kelton, John G.; Arnold, Donald M.

2014-01-01

467

Acute bone marrow edema of the hip: role of MR imaging  

Microsoft Academic Search

Acute bone marrow edema of the hip is a diagnostic challenge for both radiologists and clinicians. Marrow edema is often seen\\u000a in patients with hip pain and restriction of motion. In patients with acute non-traumatic hip pain, whose radiographs are\\u000a negative or inconclusive, MR imaging is the imaging study of choice. MR imaging is the most sensitive and specific imaging

Apostolos H. Karantanas

2007-01-01

468

Increased Failure Rate of Autologous Chondrocyte Implantation After Previous Treatment With Marrow Stimulation Techniques  

Microsoft Academic Search

Background: Marrow stimulation techniques such as drilling or microfracture are first-line treatment options for symptomatic cartilage defects. Common knowledge holds that these treatments do not compromise subsequent cartilage repair procedures with autologous chondrocyte implantation.Hypothesis: Cartilage defects pretreated with marrow stimulation techniques will have an increased failure rate.Study Design: Cohort study; Level of evidence, 2.Methods: The first 321 consecutive patients treated

Tom Minas; Andreas H. Gomoll; Ralf Rosenberger; Ronald O. Royce; Tim Bryant

2009-01-01

469

Persistence of Grazed Red Clover Varieties  

Technology Transfer Automated Retrieval System (TEKTRAN)

Historically, red clover (Trifolium pratense) has been limited by its lack of stand persistence in hay and grazed systems compared to other small-seeded forage legumes. Breeding over the past 50 years has extended red clover persistence in a hay management system to four years. This study examined g...

470

Haemorrhagic Enteropathies in Red Deer (Cervus Elaphus)  

Microsoft Academic Search

Sir, — I would like to draw your readers’ attention to the occurrence of post-stress haemorrhagic enteropathies in red deer (Cervus elaphus). Haemorrhagic enteropathy has been observed in approximately 75 percent of red deer necropsied at the Invermay Animal Health Laboratory and has also been seen in wapiti (Cervus canadensis). Deer dying with post-stress haemorrhagic enteropathy commonly have splash haemorrhages

H. J. F. McAllum

1982-01-01

471

Enhancing automated red teaming with evolvable simulation  

Microsoft Academic Search

Automated Red Teaming (ART), an automated process for Manual Red Teaming, is a technique frequently utilised by the Military Operational Analysis (OA) community to un- cover vulnerabilities in operational tactics. Currently, indi- vidual ART studies are limited to the parameter tuning of a simulation model with a fixed structure. The effects in the evolutions of structural features of a simulation

Yongliang Xu; Malcolm Yoke Hean Low; Chwee Seng Choo

2009-01-01

472

Functional aspects of red cell antigens  

Microsoft Academic Search

Over 250 blood group determinants are known and most of these are located on integral red cell proteins and glycoproteins. The functions of some of these structures are known: Diego (band 3) is the red cell anion exchanger; Kidd, a urea transporter; Colton (aquaporin 1), a water channel; Cromer (DAF) and Knops (CRI), complement regulators; Diego (band 3) and Gerbich

G. Daniels

1999-01-01

473

Do red giants have short mode lifetimes?  

E-print Network

We show evidence that the red giant star ksi Hya has an oscillation mode lifetime, tau, of about 2 days significantly shorter than predicted by theory (tau = 17 days, Houdek & Gough 2002). If this is a general trend of red giants it would limit the prospects of asteroseismology on these stars because of poor coherence of the oscillations.

D. Stello; H. Kjeldsen; T. R. Bedding; D. Buzasi

2005-10-18

474

Healthy Plate Red onion, rhubarb, radicchio,  

E-print Network

tablespoon oil 1/2 teaspoon salt 1/2 teaspoon cayenne pepper 1 teaspoon chili powder 1 teaspoon cumin tablespoons light coconut milk 1 tablespoon low sodium soy sauce 1 tablespoon toasted sesame seed oil Red red bell peppers 2 tablespoons cashews ½ cup chopped cilantro Fill remainder of jar with baby spinach

Bogyo, Matthew

475

33 CFR 117.135 - Red River.  

Code of Federal Regulations, 2011 CFR

...Navigable Waters 1 2011-07-01 2011-07-01 false Red River. 117.135 Section 117.135 Navigation and Navigable...REGULATIONS Specific Requirements Arkansas § 117.135 Red River. The draws of the bridges above mile 276.0 at the...

2011-07-01

476

33 CFR 117.135 - Red River.  

Code of Federal Regulations, 2012 CFR

...Navigable Waters 1 2012-07-01 2012-07-01 false Red River. 117.135 Section 117.135 Navigation and Navigable...REGULATIONS Specific Requirements Arkansas § 117.135 Red River. The draws of the bridges above mile 276.0 at the...

2012-07-01

477

33 CFR 117.135 - Red River.  

Code of Federal Regulations, 2010 CFR

...Navigable Waters 1 2010-07-01 2010-07-01 false Red River. 117.135 Section 117.135 Navigation and Navigable...REGULATIONS Specific Requirements Arkansas § 117.135 Red River. The draws of the bridges above mile 276.0 at the...

2010-07-01

478

33 CFR 117.491 - Red River.  

Code of Federal Regulations, 2013 CFR

...Navigable Waters 1 2013-07-01 2013-07-01 false Red River. 117.491 Section 117.491 Navigation and Navigable...REGULATIONS Specific Requirements Louisiana § 117.491 Red River. (a) The draw of the Union Pacific Railroad...

2013-07-01

479

33 CFR 117.491 - Red River.  

Code of Federal Regulations, 2010 CFR

...Navigable Waters 1 2010-07-01 2010-07-01 false Red River. 117.491 Section 117.491 Navigation and Navigable...REGULATIONS Specific Requirements Louisiana § 117.491 Red River. (a) The draw of the Union Pacific Railroad...

2010-07-01

480

33 CFR 117.135 - Red River.  

Code of Federal Regulations, 2013 CFR

...Navigable Waters 1 2013-07-01 2013-07-01 false Red River. 117.135 Section 117.135 Navigation and Navigable...REGULATIONS Specific Requirements Arkansas § 117.135 Red River. The draws of the bridges above mile 276.0 at the...

2013-07-01