76 FR 71558 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Requests...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-11-18

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [Docket No. DARS 2011-0069-0002] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Requests for Equitable Adjustment (OMB Control Number 0704-0397) AGENCY: Defense Acquisition Regulations System, Department of...

75 FR 45104 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Contract...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-02

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Contract Pricing (OMB Control Number 0704-0232) AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request for...

76 FR 35424 - Information Collection Requirements; Defense Federal Acquisition Regulation Supplement...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-06-17

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System Information Collection Requirements; Defense Federal Acquisition Regulation Supplement; Construction and Architect-Engineer Contracts AGENCY..., Construction and Architect-Engineer Contracts, and Related Clauses at DFARS 252.236; OMB Control Number 0704...

75 FR 9114 - Defense Federal Acquisition Regulation Supplement; Additional Requirements Applicable to...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-01

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System 48 CFR Part 217 Defense Federal Acquisition Regulation Supplement; Additional Requirements Applicable to Multiyear Contracts (DFARS Case 2008-D023) AGENCY: Defense Acquisition Regulations System. Department of Defense (DoD). ACTION: Interim rule...

75 FR 45104 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-08-02

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [OMB Control Number 0704-0225] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Administrative Matters... Supplement (DFARS) Part 204, Administrative Matters, and related clauses at DFARS 252.204; DD Form 2051...

78 FR 63462 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-24

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [Docket Number DARS-2013-0035] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Organizational Conflict..., Department of Defense (DoD). ACTION: Notice and request for comments regarding a proposed extension of an...

40 CFR 63.312 - Existing regulations and requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission reductions...

40 CFR 63.312 - Existing regulations and requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission reductions...

40 CFR 63.312 - Existing regulations and requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission reductions...

40 CFR 63.312 - Existing regulations and requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission reductions...

40 CFR 63.312 - Existing regulations and requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... National Emission Standards for Coke Oven Batteries § 63.312 Existing regulations and requirements. (a) The..., topside port lids, coke oven doors, and charging operations in effect on September 15, 1992, or which have... method of monitoring in effect on September 15, 1992, and that ensures coke oven emission reductions...

76 FR 39236 - Federal Acquisition Regulation; Uniform Suspension and Debarment Requirement

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-05

... 9000-AL75 Federal Acquisition Regulation; Uniform Suspension and Debarment Requirement AGENCIES... interim rule amending the Federal Acquisition Regulation (FAR) to implement section 815 of the National... Acquisition Streamlining Act of 1994 (FASA) (31 U.S.C. 6101 note) by amending the definition of ``procurement...

7 CFR 330.200 - Movement of plant pests regulated; permits required.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 5 2010-01-01 2010-01-01 false Movement of plant pests regulated; permits required... AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE FEDERAL PLANT PEST REGULATIONS; GENERAL; PLANT PESTS; SOIL, STONE, AND QUARRY PRODUCTS; GARBAGE Movement of Plant Pests § 330.200 Movement of...

76 FR 50461 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Types of...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-15

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [OMB Control Number 0704-0259] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Types of Contracts AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request...

Prediction of Regulation Reserve Requirements in California ISO Control Area based on BAAL Standard

DOE Office of Scientific and Technical Information (OSTI.GOV)

Etingov, Pavel V.; Makarov, Yuri V.; Samaan, Nader A.

This paper presents new methodologies developed at Pacific Northwest National Laboratory (PNNL) to estimate regulation capacity requirements in the California ISO control area. Two approaches have been developed: (1) an approach based on statistical analysis of actual historical area control error (ACE) and regulation data, and (2) an approach based on balancing authority ACE limit control performance standard. The approaches predict regulation reserve requirements on a day-ahead basis including upward and downward requirements, for each operating hour of a day. California ISO data has been used to test the performance of the proposed algorithms. Results show that software tool allowsmore » saving up to 30% on the regulation procurements cost .« less

78 FR 32547 - Loan Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-31

... Loan Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z); Prohibition on... Lending Act (Regulation Z) Final Rule, issued on January 20, 2013, and published in the Federal Register... Originator Compensation Requirements Under the Truth in Lending Act (Regulation Z) (Final Rule).\\2\\ The Final...

76 FR 14562 - Federal Acquisition Regulation; Additional Requirements for Market Research

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-16

... circumstances and entry of new businesses, on- going market research is not a waste of manpower and taxpayers..., Sequence 1] RIN 9000-AL50 Federal Acquisition Regulation; Additional Requirements for Market Research..., Market Research, of the National Defense Authorization Act for Fiscal Year 2008. Section 826 requires the...

78 FR 19467 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-04-01

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [Docket Number DARS-2013-0010... Mentor-Prot[eacute]g[eacute] Program AGENCY: Defense Acquisition Regulations System, Department of... Collection DFARS Appendix I-112.2(a)-(d) requires mentor firms to report on the progress made under active...

75 FR 20825 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DoD Pilot...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-04-21

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [OMB Control Number 0704-0332] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; DoD Pilot Mentor-Protege Program AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION...

77 FR 29983 - Federal Acquisition Regulation; Information Collection; Novation/Change of Name Requirements

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-21

... change to http://www.regulations.gov , including any personal and/or business confidential information...; Information Collection; Novation/ Change of Name Requirements AGENCY: Department of Defense (DOD), General... collection requirement concerning Novation/Change of Name Requirements. Public comments are particularly...

77 FR 25611 - Endangered and Threatened Wildlife and Plants; Revised Implementing Regulations for Requirements...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-05-01

... Threatened Wildlife and Plants; Revised Implementing Regulations for Requirements To Publish Textual... regulations related to publishing textual descriptions of proposed and final critical habitat boundaries in... described in the Regulation Promulgation section of a rulemaking that is published in the Federal Register...

75 FR 41161 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 251...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-07-15

...] Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Part 251, Contractor... Acquisition Regulation Supplement (DFARS) Part 251, Contractor Use of Government Supply Sources, and the... and Uses: This information collection permits contractors to-- Place orders under Federal Supply...

Cellular growth in plants requires regulation of cell wall biochemistry.

PubMed

Chebli, Youssef; Geitmann, Anja

2017-02-01

Cell and organ morphogenesis in plants are regulated by the chemical structure and mechanical properties of the extracellular matrix, the cell wall. The two primary load bearing components in the plant cell wall, the pectin matrix and the cellulose/xyloglucan network, are constantly remodelled to generate the morphological changes required during plant development. This remodelling is regulated by a plethora of loosening and stiffening agents such as pectin methyl-esterases, calcium ions, expansins, and glucanases. The tight spatio-temporal regulation of the activities of these agents is a sine qua non condition for proper morphogenesis at cell and tissue levels. The pectin matrix and the cellulose-xyloglucan network operate in concert and their behaviour is mutually dependent on their chemical, structural and mechanical modifications. Copyright © 2017 Elsevier Ltd. All rights reserved.

2 CFR 170.200 - Requirements for program announcements, regulations, and application instructions.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 2 Grants and Agreements 1 2013-01-01 2013-01-01 false Requirements for program announcements, regulations, and application instructions. 170.200 Section 170.200 Grants and Agreements Office of Management... are subject to Transparency Act reporting requirements; and (2) That either: (i) Is issued on or after...

2 CFR 170.200 - Requirements for program announcements, regulations, and application instructions.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 2 Grants and Agreements 1 2012-01-01 2012-01-01 false Requirements for program announcements, regulations, and application instructions. 170.200 Section 170.200 Grants and Agreements Office of Management... are subject to Transparency Act reporting requirements; and (2) That either: (i) Is issued on or after...

2 CFR 170.200 - Requirements for program announcements, regulations, and application instructions.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 2 Grants and Agreements 1 2014-01-01 2014-01-01 false Requirements for program announcements, regulations, and application instructions. 170.200 Section 170.200 Grants and Agreements Office of Management... Transparency Act reporting requirements; and (2) That either: (i) Is issued on or after the effective date of...

2 CFR 170.200 - Requirements for program announcements, regulations, and application instructions.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 2 Grants and Agreements 1 2011-01-01 2011-01-01 false Requirements for program announcements, regulations, and application instructions. 170.200 Section 170.200 Grants and Agreements Office of Management... Transparency Act reporting requirements; and (2) That either: (i) Is issued on or after the effective date of...

Hydrogen monitoring requirements in the global technical regulation on hydrogen and fuel cell vehicles

DOE PAGES

Buttner, William; Rivkin, C.; Burgess, R.; ...

2017-02-04

Here, the United Nations Economic Commission for Europe Global Technical Regulation (GTR) Number 13 ( Global Technical Regulation on Hydrogen and Fuel Cell Vehicles) is the defining document regulating safety requirements in hydrogen vehicles, and in particular, fuel cell electric vehicles (FCEVs). GTR Number 13 has been formally adopted and will serve as the basis for the national regulatory standards for FCEV safety in North America (led by the United States), Japan, Korea, and the European Union. The GTR defines safety requirements for these vehicles, including specifications on the allowable hydrogen levels in vehicle enclosures during in-use and post-crash conditionsmore » and on the allowable hydrogen emissions levels in vehicle exhaust during certain modes of normal operation. However, in order to be incorporated into national regulations, that is, to be legally binding, methods to verify compliance with the specific requirements must exist. In a collaborative program, the Sensor Laboratories at the National Renewable Energy Laboratory in the United States and the Joint Research Centre, Institute for Energy and Transport in the Netherlands have been evaluating and developing analytical methods that can be used to verify compliance with the hydrogen release requirements as specified in the GTR.« less

Additional requirements for charitable hospitals; community health needs assessments for charitable hospitals; requirement of a section 4959 excise tax return and time for filing the return. Final regulations and removal of temporary regulations.

PubMed

2014-12-31

This document contains final regulations that provide guidance regarding the requirements for charitable hospital organizations added by the Patient Protection and Affordable Care Act of 2010. The regulations will affect charitable hospital organizations

42 CFR 417.478 - Requirements of other laws and regulations.

Code of Federal Regulations, 2012 CFR

2012-10-01

... information; (c) Section 1301(c)(8) of the PHS Act, which relates to liability arrangements to protect... 42 Public Health 3 2012-10-01 2012-10-01 false Requirements of other laws and regulations. 417.478 Section 417.478 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN...

78 FR 72620 - Federal Acquisition Regulation; Higher-Level Contract Quality Requirements

Federal Register 2010, 2011, 2012, 2013, 2014

2013-12-03

... DEPARTMENT OF DEFENSE GENERAL SERVICES ADMINISTRATION NATIONAL AERONAUTICS AND SPACE.... IV. Regulatory Flexibility Act The Department of Defense (DoD), the General Services Administration...-AM65 Federal Acquisition Regulation; Higher-Level Contract Quality Requirements AGENCY: Department of...

Translational regulation of sigma 32 synthesis: requirement for an internal control element.

PubMed Central

Kamath-Loeb, A S; Gross, C A

1991-01-01

We have investigated the sequence requirements for the translational regulation of sigma 32 by examining the behavior of a new rpoH-lacZ protein fusion containing a short N-terminal fragment of sigma 32 fused to beta-galactosidase. Although the fusion retains rpoH translational initiation signals, it lacks translational regulation, implicating coding sequences within rpoH in this regulatory process. Images PMID:2050641

Root growth regulation and gravitropism in maize roots does not require the epidermis

NASA Technical Reports Server (NTRS)

Bjorkman, T.; Cleland, R. E.

1991-01-01

We have earlier published observations showing that endogenous alterations in growth rate during gravitropism in maize roots (Zea mays L.) are unaffected by the orientation of cuts which remove epidermal and cortical tissue in the growing zone (Bjorkman and Cleland, 1988, Planta 176, 513-518). We concluded that the epidermis and cortex are not essential for transporting a growth-regulating signal in gravitropism or straight growth, nor for regulating the rate of tissue expansion. This conclusion has been challenged by Yang et al. (1990, Planta 180, 530-536), who contend that a shallow girdle around the entire perimeter of the root blocks gravitropic curvature and that this inhibition is the result of a requirement for epidermal cells to transport the growth-regulating signal. In this paper we demonstrate that the entire epidermis can be removed without blocking gravitropic curvature and show that the position of narrow girdles does not affect the location of curvature. We therefore conclude that the epidermis is not required for transport of a growth-regulating substance from the root cap to the growing zone, nor does it regulate the growth rate of the elongating zone of roots.

The Future Impact of Wind on BPA Power System Load Following and Regulation Requirements

DOE Office of Scientific and Technical Information (OSTI.GOV)

Makarov, Yuri V.; Lu, Shuai; McManus, Bart

Wind power is growing in a very fast pace as an alternative generating resource. As the ratio of wind power over total system capacity increases, the impact of wind on various system aspects becomes significant. This paper presents a methodology to study the future impact of wind on BPA power system load following and regulation requirements. Existing methodologies for similar analysis include dispatch model simulation and standard deviation evaluation on load and wind data. The methodology proposed in this paper uses historical data and stochastic processes to simulate the load balancing processes in the BPA power system. It mimics themore » actual power system operations therefore the results are close to reality yet the study based on this methodology is convenient to perform. The capacity, ramp rate and ramp duration characteristics are extracted from the simulation results. System load following and regulation capacity requirements are calculated accordingly. The ramp rate and ramp duration data obtained from the analysis can be used to evaluate generator response or maneuverability requirement and regulating units’ energy requirement, respectively.« less

50 CFR 86.55 - What are my compliance requirements with Federal laws, regulations, and policies?

Code of Federal Regulations, 2010 CFR

2010-10-01

... Federal laws, regulations, and policies? 86.55 Section 86.55 Wildlife and Fisheries UNITED STATES FISH AND... compliance requirements with Federal laws, regulations, and policies? (a) To receive Federal funds, you must agree to and certify compliance with all applicable Federal laws, regulations, and policies. You must...

50 CFR 86.55 - What are my compliance requirements with Federal laws, regulations, and policies?

Code of Federal Regulations, 2013 CFR

2013-10-01

... Federal laws, regulations, and policies? 86.55 Section 86.55 Wildlife and Fisheries UNITED STATES FISH AND... compliance requirements with Federal laws, regulations, and policies? (a) To receive Federal funds, you must agree to and certify compliance with all applicable Federal laws, regulations, and policies. You must...

50 CFR 86.55 - What are my compliance requirements with Federal laws, regulations, and policies?

Code of Federal Regulations, 2014 CFR

2014-10-01

... Federal laws, regulations, and policies? 86.55 Section 86.55 Wildlife and Fisheries UNITED STATES FISH AND... compliance requirements with Federal laws, regulations, and policies? (a) To receive Federal funds, you must agree to and certify compliance with all applicable Federal laws, regulations, and policies. You must...

50 CFR 86.55 - What are my compliance requirements with Federal laws, regulations, and policies?

Code of Federal Regulations, 2011 CFR

2011-10-01

... Federal laws, regulations, and policies? 86.55 Section 86.55 Wildlife and Fisheries UNITED STATES FISH AND... compliance requirements with Federal laws, regulations, and policies? (a) To receive Federal funds, you must agree to and certify compliance with all applicable Federal laws, regulations, and policies. You must...

50 CFR 86.55 - What are my compliance requirements with Federal laws, regulations, and policies?

Code of Federal Regulations, 2012 CFR

2012-10-01

... Federal laws, regulations, and policies? 86.55 Section 86.55 Wildlife and Fisheries UNITED STATES FISH AND... compliance requirements with Federal laws, regulations, and policies? (a) To receive Federal funds, you must agree to and certify compliance with all applicable Federal laws, regulations, and policies. You must...

27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

Code of Federal Regulations, 2014 CFR

2014-04-01

... Provisions Relating to Distilled Spirits, Wines, and Beer § 70.411 Imposition of taxes, qualification... distilled spirits (including alcohol), wine and beer. (b) Qualification requirements. Distillers, winemakers... beer which are within the jurisdiction of TTB are published in the regulations described in this...

27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

Code of Federal Regulations, 2013 CFR

2013-04-01

... Provisions Relating to Distilled Spirits, Wines, and Beer § 70.411 Imposition of taxes, qualification... distilled spirits (including alcohol), wine and beer. (b) Qualification requirements. Distillers, winemakers... beer which are within the jurisdiction of TTB are published in the regulations described in this...

27 CFR 70.411 - Imposition of taxes, qualification requirements, and regulations.

Code of Federal Regulations, 2011 CFR

2011-04-01

... Provisions Relating to Distilled Spirits, Wines, and Beer § 70.411 Imposition of taxes, qualification... distilled spirits (including alcohol), wine and beer. (b) Qualification requirements. Distillers, winemakers... beer which are within the jurisdiction of TTB are published in the regulations described in this...

76 FR 28405 - Endangered and Threatened Wildlife and Plants; Revised Implementing Regulations for Requirements...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-17

... and Threatened Wildlife and Plants; Revised Implementing Regulations for Requirements To Publish... as the Services) propose to revise regulations related to publishing textual descriptions of proposed... published in the Federal Register will be the official delineation of the designation, and we will continue...

GLUT4 Retention in Adipocytes Requires Two Intracellular Insulin-regulated Transport Steps

PubMed Central

Zeigerer, Anja; Lampson, Michael A.; Karylowski, Ola; Sabatini, David D.; Adesnik, Milton; Ren, Mindong; McGraw, Timothy E.

2002-01-01

Insulin regulates glucose uptake into fat and muscle by modulating the distribution of the GLUT4 glucose transporter between the surface and interior of cells. The GLUT4 trafficking pathway overlaps with the general endocytic recycling pathway, but the degree and functional significance of the overlap are not known. In this study of intact adipocytes, we demonstrate, by using a compartment-specific fluorescence-quenching assay, that GLUT4 is equally distributed between two intracellular pools: the transferrin receptor-containing endosomes and a specialized compartment that excludes the transferrin receptor. These pools of GLUT4 are in dynamic communication with one another and with the cell surface. Insulin-induced redistribution of GLUT4 to the surface requires mobilization of both pools. These data establish a role for the general endosomal system in the specialized, insulin-regulated trafficking of GLUT4. Trafficking through the general endosomal system is regulated by rab11. Herein, we show that rab11 is required for the transport of GLUT4 from endosomes to the specialized compartment and for the insulin-induced translocation to the cell surface, emphasizing the importance of the general endosomal pathway in the specialized trafficking of GLUT4. Based on these findings we propose a two-step model for GLUT4 trafficking in which the general endosomal recycling compartment plays a specialized role in the insulin-regulated traffic of GLUT4. This compartment-based model provides the framework for understanding insulin-regulated trafficking at a molecular level. PMID:12134080

GLUT4 retention in adipocytes requires two intracellular insulin-regulated transport steps.

PubMed

Zeigerer, Anja; Lampson, Michael A; Karylowski, Ola; Sabatini, David D; Adesnik, Milton; Ren, Mindong; McGraw, Timothy E

2002-07-01

Insulin regulates glucose uptake into fat and muscle by modulating the distribution of the GLUT4 glucose transporter between the surface and interior of cells. The GLUT4 trafficking pathway overlaps with the general endocytic recycling pathway, but the degree and functional significance of the overlap are not known. In this study of intact adipocytes, we demonstrate, by using a compartment-specific fluorescence-quenching assay, that GLUT4 is equally distributed between two intracellular pools: the transferrin receptor-containing endosomes and a specialized compartment that excludes the transferrin receptor. These pools of GLUT4 are in dynamic communication with one another and with the cell surface. Insulin-induced redistribution of GLUT4 to the surface requires mobilization of both pools. These data establish a role for the general endosomal system in the specialized, insulin-regulated trafficking of GLUT4. Trafficking through the general endosomal system is regulated by rab11. Herein, we show that rab11 is required for the transport of GLUT4 from endosomes to the specialized compartment and for the insulin-induced translocation to the cell surface, emphasizing the importance of the general endosomal pathway in the specialized trafficking of GLUT4. Based on these findings we propose a two-step model for GLUT4 trafficking in which the general endosomal recycling compartment plays a specialized role in the insulin-regulated traffic of GLUT4. This compartment-based model provides the framework for understanding insulin-regulated trafficking at a molecular level.

24 CFR 3280.3 - Manufactured home procedural and enforcement regulations and consumer manual requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 5 2010-04-01 2010-04-01 false Manufactured home procedural and enforcement regulations and consumer manual requirements. 3280.3 Section 3280.3 Housing and Urban Development... consumer manual requirements. A manufacturer must comply with the requirements of this part 3280, part 3282...

78 FR 27908 - Federal Management Regulation (FMR); Mail Management; Financial Requirements for All Agencies

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-13

...; Sequence 1] RIN 3090-AI79 Federal Management Regulation (FMR); Mail Management; Financial Requirements for... Services Administration (GSA) is proposing to amend the Federal Management Regulation (FMR) by revising its mail management policy. A major part of the proposed revision involves the removal of the agency...

12 CFR 350.12 - Disclosure required by applicable banking or securities law or regulations.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Disclosure required by applicable banking or securities law or regulations. 350.12 Section 350.12 Banks and Banking FEDERAL DEPOSIT INSURANCE CORPORATION... STATE NONMEMBER BANKS § 350.12 Disclosure required by applicable banking or securities law or...

78 FR 17186 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-03-20

... Requirements for Critical Safety Items AGENCY: Defense Acquisition Regulations System, Department of Defense... of the burden of the proposed information collection; (c) ways to enhance the quality, utility, and... technology. The Office of Management and Budget (OMB) has approved this information collection for use...

GABAA Receptor Regulation of Voluntary Ethanol Drinking Requires PKCε

PubMed Central

Besheer, Joyce; Lepoutre, Veronique; Mole, Beth; Hodge, Clyde W.

2010-01-01

Protein kinase C (PKC) regulates a variety of neural functions, including ion channel activity, neurotransmitter release, receptor desensitization and differentiation. We have shown previously that mice lacking the ε-isoform of PKC (PKCε) self-administer 75% less ethanol and exhibit supersensitivity to acute ethanol and allosteric positive modulators of GABAA receptors when compared with wild-type controls. The purpose of the present study was to examine involvement of PKCε in GABAA receptor regulation of voluntary ethanol drinking. To address this question, PKCε null-mutant and wild-type control mice were allowed to drink ethanol (10% v/v) vs. water on a two-bottle continuous access protocol. The effects of diazepam (nonselective GABAA BZ positive modulator), zolpidem (GABAA α1 agonist), L-655,708 (BZ-sensitive GABAA α5 inverse agonist), and flumazenil (BZ antagonist) were then tested on ethanol drinking. Ethanol intake (grams/kg/day) by wild-type mice decreased significantly after diazepam or zolpidem but increased after L-655,708 administration. Flumazenil antagonized diazepam-induced reductions in ethanol drinking in wild-type mice. However, ethanol intake by PKCε null mice was not altered by any of the GABAergic compounds even though effects were seen on water drinking in these mice. Increased acute sensitivity to ethanol and diazepam, which was previously reported, was confirmed in PKCε null mice. Thus, results of the present study show that PKCε null mice do not respond to doses of GABAA BZ receptor ligands that regulate ethanol drinking by wild-type control mice. This suggests that PKCε may be required for GABAA receptor regulation of chronic ethanol drinking. PMID:16881070

25 CFR 518.7 - If a tribe holds a certificate of self-regulation, is it required to report information to the...

Code of Federal Regulations, 2010 CFR

2010-04-01

... 25 Indians 2 2010-04-01 2010-04-01 false If a tribe holds a certificate of self-regulation, is it... REGULATION OF CLASS II GAMING § 518.7 If a tribe holds a certificate of self-regulation, is it required to... certificate of self-regulation shall be required to submit a self-regulation report annually to the Commission...

76 FR 1360 - Regulated Navigation Area; Reporting Requirements for Barges Loaded With Certain Dangerous...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-01-10

... requirements under the Regulated Navigation Area (RNA) established by 33 CFR 165.830 for barges loaded with... the RNA. This suspension of the CDC reporting requirements in no way relieves towing vessel operators and fleeting area managers responsible for CDC barges in the RNA from their dangerous cargo or vessel...

50 CFR 14.254 - What are the requirements contained in these regulations?

Code of Federal Regulations, 2010 CFR

2010-10-01

... 50 Wildlife and Fisheries 1 2010-10-01 2010-10-01 false What are the requirements contained in these regulations? 14.254 Section 14.254 Wildlife and Fisheries UNITED STATES FISH AND WILDLIFE SERVICE... IMPORTATION OF WILDLIFE AND PLANTS IMPORTATION, EXPORTATION, AND TRANSPORTATION OF WILDLIFE Captive Wildlife...

Endocytosis-dependent coordination of multiple actin regulators is required for wound healing

PubMed Central

Matsubayashi, Yutaka; Coulson-Gilmer, Camilla

2015-01-01

The ability to heal wounds efficiently is essential for life. After wounding of an epithelium, the cells bordering the wound form dynamic actin protrusions and/or a contractile actomyosin cable, and these actin structures drive wound closure. Despite their importance in wound healing, the molecular mechanisms that regulate the assembly of these actin structures at wound edges are not well understood. In this paper, using Drosophila melanogaster embryos, we demonstrate that Diaphanous, SCAR, and WASp play distinct but overlapping roles in regulating actin assembly during wound healing. Moreover, we show that endocytosis is essential for wound edge actin assembly and wound closure. We identify adherens junctions (AJs) as a key target of endocytosis during wound healing and propose that endocytic remodeling of AJs is required to form “signaling centers” along the wound edge that control actin assembly. We conclude that coordination of actin assembly, AJ remodeling, and membrane traffic is required for the construction of a motile leading edge during wound healing. PMID:26216900

76 FR 78126 - Disclosure Requirements for Depository Institutions Lacking Federal Deposit Insurance (Regulation I)

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-16

... and Comment The Administrative Procedure Act (APA) \\11\\ generally requires public notice and an opportunity to comment before promulgation of substantive regulations.\\12\\ The APA provides exceptions to... APA's notice-and-comment requirements. \\11\\ 5 U.S.C. 551 et seq. \\12\\ 5 U.S.C. 553(b), (c). \\13\\ 5 U.S...

33 CFR 165.921 - Regulated Navigation Area; Reporting Requirements for Barges Loaded with Certain Dangerous...

Code of Federal Regulations, 2013 CFR

2013-07-01

... RNA must report the information required by this section as set out in table 165.921(g) to this...) Regulated Navigation Area. The following waters are a regulated navigation area (RNA): the Illinois Waterway... RNA. This section does not apply to towing vessel operators responsible for barges not carrying CDC...

33 CFR 165.921 - Regulated Navigation Area; Reporting Requirements for Barges Loaded with Certain Dangerous...

Code of Federal Regulations, 2010 CFR

2010-07-01

... RNA must report the information required by this section as set out in table 165.921(g) to this...) Regulated Navigation Area. The following waters are a regulated navigation area (RNA): the Illinois Waterway... RNA. This section does not apply to towing vessel operators responsible for barges not carrying CDC...

33 CFR 165.921 - Regulated Navigation Area; Reporting Requirements for Barges Loaded with Certain Dangerous...

Code of Federal Regulations, 2011 CFR

2011-07-01

... RNA must report the information required by this section as set out in table 165.921(g) to this...) Regulated Navigation Area. The following waters are a regulated navigation area (RNA): the Illinois Waterway... RNA. This section does not apply to towing vessel operators responsible for barges not carrying CDC...

33 CFR 165.921 - Regulated Navigation Area; Reporting Requirements for Barges Loaded with Certain Dangerous...

Code of Federal Regulations, 2014 CFR

2014-07-01

... RNA must report the information required by this section as set out in table 165.921(g) to this...) Regulated Navigation Area. The following waters are a regulated navigation area (RNA): the Illinois Waterway... RNA. This section does not apply to towing vessel operators responsible for barges not carrying CDC...

33 CFR 165.921 - Regulated Navigation Area; Reporting Requirements for Barges Loaded with Certain Dangerous...

Code of Federal Regulations, 2012 CFR

2012-07-01

... RNA must report the information required by this section as set out in table 165.921(g) to this...) Regulated Navigation Area. The following waters are a regulated navigation area (RNA): the Illinois Waterway... RNA. This section does not apply to towing vessel operators responsible for barges not carrying CDC...

Eph receptor interclass cooperation is required for the regulation of cell proliferation

DOE Office of Scientific and Technical Information (OSTI.GOV)

Jurek, Aleksandra; Genander, Maria; Kundu, Parag

2016-10-15

Cancer often arises by the constitutive activation of mitogenic pathways by mutations in stem cells. Eph receptors are unusual in that although they regulate the proliferation of stem/progenitor cells in many adult organs, they typically fail to transform cells. Multiple ephrins and Eph receptors are often co-expressed and are thought to be redundant, but we here describe an unexpected dichotomy with two homologous ligands, ephrin-B1 and ephrin-B2, regulating specifically migration or proliferation in the intestinal stem cell niche. We demonstrate that the combined activity of two different coexpressed Eph receptors of the A and B class assembled into common signalingmore » clusters in response to ephrin-B2 is required for mitogenic signaling. The requirement of two different Eph receptors to convey mitogenic signals identifies a new type of cooperation within this receptor family and helps explain why constitutive activation of a single receptor fails to transform cells. - Highlights: • We demonstrate that ephrin-B1 and ephrin-B2 have largely non-overlapping functions in the intestinal stem cell niche. • Ephrin-B1 regulates cell positioning and ephrin-B2 regulates cell proliferation in the intestinal stem cell niche. • EphA4/B2 receptor cooperation in response to ephrin-B2 binding is obligatory to convey mitogenic signals in the intestine. • EphA4 facilitates EphB2 phosphorylation in response to ephrin-B2 in SW480 adenocarcinoma cells. • Ephrin-B1 and ephrin-B2 induce phosphorylation and degradation of the EphB2 receptor with different kinetics.« less

CCAAT/Enhancer Binding Protein–α Regulates the Protease/Antiprotease Balance Required for Bronchiolar Epithelium Regeneration

PubMed Central

Sato, Atsuyasu; Xu, Yan; Whitsett, Jeffrey A.

2012-01-01

Many transcription factors that regulate lung morphogenesis during development are reactivated to mediate repairs of the injured adult lung. We hypothesized that CCAAT/enhancer binding protein–α (C/EBPα), a transcription factor critical for perinatal lung maturation, regulates genes required for the normal repair of the bronchiolar epithelium after injury. Transgenic CebpαΔ/Δ mice, in which Cebpa was conditionally deleted from Clara cells and Type II cells after birth, were used in this study. Airway injury was induced in mice by the intraperitoneal administration of naphthalene to ablate bronchiolar epithelial cells. Although the deletion of C/EBPα did not influence lung structure and function under unstressed conditions, C/EBPα was required for the normal repair of terminal bronchiolar epithelium after naphthalene injury. To identify cellular processes that are influenced by C/EBPα during repair, mRNA microarray was performed on terminal bronchiolar epithelial cells isolated by laser-capture microdissection. Normal repair of the terminal bronchiolar epithelium was highly associated with the mRNAs regulating antiprotease activities, and their induction required C/EBPα. The defective deposition of fibronectin in CebpαΔ/Δ mice was associated with increased protease activity and delayed differentiation of FoxJ1-expressing ciliated cells. The fibronectin and ciliated cells were restored by the intratracheal treatment of CebpαΔ/Δ mice with the serine protease inhibitor. In conclusion, C/EBPα regulates the expression of serine protease inhibitors that are required for the normal increase of fibronectin and the restoration of ciliated cells after injury. Treatment with serine protease inhibitor may aid in the recovery of injured bronchiolar epithelial cells, and prevent common chronic lung diseases. PMID:22652201

77 FR 34315 - National Pollutant Discharge Elimination System-Proposed Regulations to Establish Requirements...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-11

...-4] RIN 2040-AE95 National Pollutant Discharge Elimination System--Proposed Regulations to Establish Requirements for Cooling Water Intake Structures at Existing Facilities; Notice of Data Availability Related to... Data Availability. SUMMARY: On April 20, 2011, EPA published proposed standards for cooling water...

Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator.

PubMed

Hollands, Kerry; Sevostiyanova, Anastasia; Groisman, Eduardo A

2014-05-13

Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understanding how such terminators can be regulated in response to specific signals. Here, we identify an extraordinarily long-lived pause at the site where Rho terminates transcription in the 5'-leader region of the Mg(2+) transporter gene mgtA in Salmonella enterica. We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the remarkable longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg(2+) availability. Unlike Rho-dependent terminators described previously, where termination occurs at multiple pause sites, there is a single site of transcription termination directed by Rho in the mgtA leader. Our data suggest that Rho-dependent termination events that are subject to regulation may require elements distinct from those operating at constitutive Rho-dependent terminators.

Unusually long-lived pause required for regulation of a Rho-dependent transcription terminator

PubMed Central

Hollands, Kerry; Sevostiyanova, Anastasia; Groisman, Eduardo A.

2014-01-01

Up to half of all transcription termination events in bacteria rely on the RNA-dependent helicase Rho. However, the nucleic acid sequences that promote Rho-dependent termination remain poorly characterized. Defining the molecular determinants that confer Rho-dependent termination is especially important for understanding how such terminators can be regulated in response to specific signals. Here, we identify an extraordinarily long-lived pause at the site where Rho terminates transcription in the 5′-leader region of the Mg2+ transporter gene mgtA in Salmonella enterica. We dissect the sequence elements required for prolonged pausing in the mgtA leader and establish that the remarkable longevity of this pause is required for a riboswitch to stimulate Rho-dependent termination in the mgtA leader region in response to Mg2+ availability. Unlike Rho-dependent terminators described previously, where termination occurs at multiple pause sites, there is a single site of transcription termination directed by Rho in the mgtA leader. Our data suggest that Rho-dependent termination events that are subject to regulation may require elements distinct from those operating at constitutive Rho-dependent terminators. PMID:24778260

77 FR 34927 - National Pollutant Discharge Elimination System-Proposed Regulations To Establish Requirements...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-06-12

...-5] RIN 2040-AE95 National Pollutant Discharge Elimination System--Proposed Regulations To Establish Requirements for Cooling Water Intake Structures at Existing Facilities; Notice of Data Availability Related to... availability. SUMMARY: On April 20, 2011, EPA published proposed standards for cooling water intake structures...

Melanocortin 4 receptor is not required for estrogenic regulations on energy homeostasis and reproduction.

PubMed

Xu, Pingwen; Zhu, Liangru; Saito, Kenji; Yang, Yongjie; Wang, Chunmei; He, Yanlin; Yan, Xiaofeng; Hyseni, Ilirjana; Tong, Qingchun; Xu, Yong

2017-05-01

Brain estrogen receptor-α (ERα) is essential for estrogenic regulation of energy homeostasis and reproduction. We previously showed that ERα expressed by pro-opiomelanocortin (POMC) neurons mediates estrogen's effects on food intake, body weight, negative regulation of hypothalamic-pituitary-gonadal axis (HPG axis) and fertility. We report here that global deletion of a key downstream receptor for POMC peptide, the melanocortin 4 receptor (MC4R), did not affect normal negative feedback regulation of estrogen on the HPG axis, estrous cyclicity and female fertility. Furthermore, loss of the MC4R did not influence estrogenic regulation on food intake and body weight. These results indicate that the MC4R is not required for estrogen's effects on metabolic and reproductive functions. Copyright © 2016 Elsevier Inc. All rights reserved.

78 FR 4725 - Escrow Requirements Under the Truth in Lending Act (Regulation Z)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-22

...The Bureau of Consumer Financial Protection (Bureau) is publishing a final rule that amends Regulation Z (Truth in Lending) to implement certain amendments to the Truth in Lending Act made by the Dodd-Frank Wall Street Reform and Consumer Protection Act (Dodd-Frank Act). Regulation Z currently requires creditors to establish escrow accounts for higher-priced mortgage loans secured by a first lien on a principal dwelling. The rule implements statutory changes made by the Dodd-Frank Act that lengthen the time for which a mandatory escrow account established for a higher-priced mortgage loan must be maintained. The rule also exempts certain transactions from the statute's escrow requirement. The primary exemption applies to mortgage transactions extended by creditors that operate predominantly in rural or underserved areas, originate a limited number of first-lien covered transactions, have assets below a certain threshold, and do not maintain escrow accounts on mortgage obligations they currently service.

75 FR 34277 - Federal Acquisition Regulation; FAR Case 2008-007, Additional Requirements for Market Research

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-16

...The Civilian Agency Acquisition Council and the Defense Acquisition Regulations Council (Councils) have agreed on an interim rule amending the Federal Acquisition Regulation (FAR) to implement Section 826 of the National Defense Authorization Act for Fiscal Year 2008 (FY08 NDAA). Section 826 established additional requirements in subsection (c) of 10 U.S.C. 2377. As a matter of policy, these requirements are extended to all executive agencies. Specifically, the head of the agency must conduct market research before issuing an indefinite-delivery indefinite-quantity (ID/IQ) task or delivery order for a noncommercial item in excess of the simplified acquisition threshold. In addition, a prime contractor with a contract in excess of $5 million for the procurement of items other than commercial items is required to conduct market research before making purchases that exceed the simplified acquisition threshold for or on behalf of the Government.

76 FR 78933 - Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-20

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0002] Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good Clinical Practice; Public Workshop AGENCY: Food and Drug Administration, HHS. ACTION: Notice of public workshop. The...

76 FR 17138 - Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-03-28

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2011-N-0002] Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good Clinical Practice; Public Workshop AGENCY: Food and Drug Administration, HHS. ACTION: Notice of public workshop. The...

75 FR 14448 - Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-03-25

... DEPARTMENT OF HEALTH AND HUMAN SERVICES Food and Drug Administration [Docket No. FDA-2010-N-0001] Food and Drug Administration Clinical Trial Requirements, Regulations, Compliance, and Good Clinical Practices; Public Workshop AGENCY: Food and Drug Administration, HHS. ACTION: Notice of public workshop...

78 FR 65218 - Defense Federal Acquisition Regulation Supplement: Private Sector Notification Requirements of In...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-31

... Federal Acquisition Regulation Supplement: Private Sector Notification Requirements of In-Sourcing Actions... Supplement (DFARS) to implement a section of the National Defense Authorization Act regarding private sector... section 938 of the National Defense Authorization Act (NDAA) for Fiscal Year 2012 regarding private sector...

Agreement that the PSD Regulations Require a Source to Commence Construction

EPA Pesticide Factsheets

This document may be of assistance in applying the New Source Review (NSR) air permitting regulations including the Prevention of Significant Deterioration (PSD) requirements. This document is part of the NSR Policy and Guidance Database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

75 FR 60263 - Federal Acquisition Regulation; Offering a Construction Requirement-8(a) Program

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-29

... geographical area where the work is to be performed. However, the SBA regulation requires the offering letters... September 30, 1993. This rule is not a major rule under 5 U.S.C. 804. B. Regulatory Flexibility Act The Regulatory Flexibility Act does not apply to this rule. This final rule does not constitute a significant FAR...

Five Conditions Commonly Used to Down-regulate Tor Complex 1 Generate Different Physiological Situations Exhibiting Distinct Requirements and Outcomes*

PubMed Central

Tate, Jennifer J.; Cooper, Terrance G.

2013-01-01

Five different physiological conditions have been used interchangeably to establish the sequence of molecular events needed to achieve nitrogen-responsive down-regulation of TorC1 and its subsequent regulation of downstream reporters: nitrogen starvation, methionine sulfoximine (Msx) addition, nitrogen limitation, rapamycin addition, and leucine starvation. Therefore, we tested a specific underlying assumption upon which the interpretation of data generated by these five experimental perturbations is premised. It is that they generate physiologically equivalent outcomes with respect to TorC1, i.e. its down-regulation as reflected by TorC1 reporter responses. We tested this assumption by performing head-to-head comparisons of the requirements for each condition to achieve a common outcome for a downstream proxy of TorC1 inactivation, nuclear Gln3 localization. We demonstrate that the five conditions for down-regulating TorC1 do not elicit physiologically equivalent outcomes. Four of the methods exhibit hierarchical Sit4 and PP2A phosphatase requirements to elicit nuclear Gln3-Myc13 localization. Rapamycin treatment required Sit4 and PP2A. Nitrogen limitation and short-term nitrogen starvation required only Sit4. G1 arrest-correlated, long-term nitrogen starvation and Msx treatment required neither PP2A nor Sit4. Starving cells of leucine or treating them with leucyl-tRNA synthetase inhibitors did not elicit nuclear Gln3-Myc13 localization. These data indicate that the five commonly used nitrogen-related conditions of down-regulating TorC1 are not physiologically equivalent and minimally involve partially differing regulatory mechanisms. Further, identical requirements for Msx treatment and long-term nitrogen starvation raise the possibility that their effects are achieved through a common regulatory pathway with glutamine, a glutamate or glutamine metabolite level as the sensed metabolic signal. PMID:23935103

78 FR 60216 - Regulated Navigation Area; Reporting Requirements for Barges Loaded With Certain Dangerous...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-10-01

... published stay (suspension) of reporting requirements under the Regulated Navigation Area (RNA) established... fleeting area managers responsible for CDC barges in the RNA from their dangerous cargo or vessel arrival... preamble as being available in the docket are part of docket USCG-2013-0760. To view documents mentioned in...

78 FR 25 - Regulated Navigation Area; Reporting Requirements for Barges Loaded With Certain Dangerous...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-01-02

... published stay (suspension) of reporting requirements under the Regulated Navigation Area (RNA) established... vessel operators and fleeting area managers responsible for CDC barges in the RNA from their dangerous...: Documents indicated in this preamble as being available in the docket are part of docket USCG-2012-1074. To...

14 CFR 11.61 - May I ask FAA to adopt, amend, or repeal a regulation, or grant relief from the requirements of a...

Code of Federal Regulations, 2010 CFR

2010-01-01

... 14 Aeronautics and Space 1 2010-01-01 2010-01-01 false May I ask FAA to adopt, amend, or repeal a regulation, or grant relief from the requirements of a current regulation? 11.61 Section 11.61 Aeronautics... to adopt, amend, or repeal a regulation, or grant relief from the requirements of a current...

A mechanism regulating G protein-coupled receptor signaling that requires cycles of protein palmitoylation and depalmitoylation.

PubMed

Jia, Lixia; Chisari, Mariangela; Maktabi, Mohammad H; Sobieski, Courtney; Zhou, Hao; Konopko, Aaron M; Martin, Brent R; Mennerick, Steven J; Blumer, Kendall J

2014-02-28

Reversible attachment and removal of palmitate or other long-chain fatty acids on proteins has been hypothesized, like phosphorylation, to control diverse biological processes. Indeed, palmitate turnover regulates Ras trafficking and signaling. Beyond this example, however, the functions of palmitate turnover on specific proteins remain poorly understood. Here, we show that a mechanism regulating G protein-coupled receptor signaling in neuronal cells requires palmitate turnover. We used hexadecyl fluorophosphonate or palmostatin B to inhibit enzymes in the serine hydrolase family that depalmitoylate proteins, and we studied R7 regulator of G protein signaling (RGS)-binding protein (R7BP), a palmitoylated allosteric modulator of R7 RGS proteins that accelerate deactivation of Gi/o class G proteins. Depalmitoylation inhibition caused R7BP to redistribute from the plasma membrane to endomembrane compartments, dissociated R7BP-bound R7 RGS complexes from Gi/o-gated G protein-regulated inwardly rectifying K(+) (GIRK) channels and delayed GIRK channel closure. In contrast, targeting R7BP to the plasma membrane with a polybasic domain and an irreversibly attached lipid instead of palmitate rendered GIRK channel closure insensitive to depalmitoylation inhibitors. Palmitate turnover therefore is required for localizing R7BP to the plasma membrane and facilitating Gi/o deactivation by R7 RGS proteins on GIRK channels. Our findings broaden the scope of biological processes regulated by palmitate turnover on specific target proteins. Inhibiting R7BP depalmitoylation may provide a means of enhancing GIRK activity in neurological disorders.

Sterol homeostasis requires regulated degradation of squalene monooxygenase by the ubiquitin ligase Doa10/Teb4

PubMed Central

Foresti, Ombretta; Ruggiano, Annamaria; Hannibal-Bach, Hans K; Ejsing, Christer S; Carvalho, Pedro

2013-01-01

Sterol homeostasis is essential for the function of cellular membranes and requires feedback inhibition of HMGR, a rate-limiting enzyme of the mevalonate pathway. As HMGR acts at the beginning of the pathway, its regulation affects the synthesis of sterols and of other essential mevalonate-derived metabolites, such as ubiquinone or dolichol. Here, we describe a novel, evolutionarily conserved feedback system operating at a sterol-specific step of the mevalonate pathway. This involves the sterol-dependent degradation of squalene monooxygenase mediated by the yeast Doa10 or mammalian Teb4, a ubiquitin ligase implicated in a branch of the endoplasmic reticulum (ER)-associated protein degradation (ERAD) pathway. Since the other branch of ERAD is required for HMGR regulation, our results reveal a fundamental role for ERAD in sterol homeostasis, with the two branches of this pathway acting together to control sterol biosynthesis at different levels and thereby allowing independent regulation of multiple products of the mevalonate pathway. DOI: http://dx.doi.org/10.7554/eLife.00953.001 PMID:23898401

78 FR 69267 - Defense Federal Acquisition Regulation Supplement: Requirements Relating to Supply Chain Risk...

Federal Register 2010, 2011, 2012, 2013, 2014

2013-11-18

... Regulation Supplement: Requirements Relating to Supply Chain Risk (DFARS Case 2012-D050) AGENCY: Defense... for FY 2013. This interim rule allows DoD to consider the impact of supply chain risk in specified... Information Relating to Supply Chain Risk,'' as amended by section 806 of the NDAA for FY 2013 (Pub. L. 112...

A Requirement for Mena, an Actin Regulator, in Local mRNA Translation in Developing Neurons.

PubMed

Vidaki, Marina; Drees, Frauke; Saxena, Tanvi; Lanslots, Erwin; Taliaferro, Matthew J; Tatarakis, Antonios; Burge, Christopher B; Wang, Eric T; Gertler, Frank B

2017-08-02

During neuronal development, local mRNA translation is required for axon guidance and synaptogenesis, and dysregulation of this process contributes to multiple neurodevelopmental and cognitive disorders. However, regulation of local protein synthesis in developing axons remains poorly understood. Here, we uncover a novel role for the actin-regulatory protein Mena in the formation of a ribonucleoprotein complex that involves the RNA-binding proteins HnrnpK and PCBP1 and regulates local translation of specific mRNAs in developing axons. We find that translation of dyrk1a, a Down syndrome- and autism spectrum disorders-related gene, is dependent on Mena, both in steady-state conditions and upon BDNF stimulation. We identify hundreds of additional mRNAs that associate with the Mena complex, suggesting that it plays broader role(s) in post-transcriptional gene regulation. Our work establishes a dual role for Mena in neurons, providing a potential link between regulation of actin dynamics and local translation. Copyright © 2017 Elsevier Inc. All rights reserved.

Healthcare Coverage and Disability Evaluation for Reserve Component Personnel: Research for the 11th Quadrennial Review of Military Compensation

DTIC Science & Technology

2012-01-01

personnel (E-5–E-9), and all warrant and commis- sioned officers. There is some variation across the services, especially for junior enlisted...the PTSD cases in 2007 and 2008. After the policy memo directing a minimum tempo - rary rating of 50 percent, the ratings for cases involving PTSD...dles the medical evaluation (these results are given in the Appendix). The regression analysis controls for this variation , so the RC-AC difference

Cryptococcus neoformans Mediator Protein Ssn8 Negatively Regulates Diverse Physiological Processes and Is Required for Virulence

PubMed Central

Wang, Lin-Ing; Lin, Yu-Sheng; Liu, Kung-Hung; Jong, Ambrose Y.; Shen, Wei-Chiang

2011-01-01

Cryptococcus neoformans is a ubiquitously distributed human pathogen. It is also a model system for studying fungal virulence, physiology and differentiation. Light is known to inhibit sexual development via the evolutionarily conserved white collar proteins in C. neoformans. To dissect molecular mechanisms regulating this process, we have identified the SSN8 gene whose mutation suppresses the light-dependent CWC1 overexpression phenotype. Characterization of sex-related phenotypes revealed that Ssn8 functions as a negative regulator in both heterothallic a-α mating and same-sex mating processes. In addition, Ssn8 is involved in the suppression of other physiological processes including invasive growth, and production of capsule and melanin. Interestingly, Ssn8 is also required for the maintenance of cell wall integrity and virulence. Our gene expression studies confirmed that deletion of SSN8 results in de-repression of genes involved in sexual development and melanization. Epistatic and yeast two hybrid studies suggest that C. neoformans Ssn8 plays critical roles downstream of the Cpk1 MAPK cascade and Ste12 and possibly resides at one of the major branches downstream of the Cwc complex in the light-mediated sexual development pathway. Taken together, our studies demonstrate that the conserved Mediator protein Ssn8 functions as a global regulator which negatively regulates diverse physiological and developmental processes and is required for virulence in C. neoformans. PMID:21559476

13 CFR 120.1511 - Certification and other reporting and notification requirements for Other Regulated SBLCs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false Certification and other reporting and notification requirements for Other Regulated SBLCs. 120.1511 Section 120.1511 Business Credit and Assistance SMALL BUSINESS ADMINISTRATION BUSINESS LOANS Risk-Based Lender Oversight Enforcement Actions § 120...

49 CFR 195.11 - What is a regulated rural gathering line and what requirements apply?

Code of Federal Regulations, 2011 CFR

2011-10-01

... subpart H of this part, except corrosion control is not required for pipelines existing on July 3, 2008... (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF HAZARDOUS LIQUIDS BY PIPELINE General § 195.11 What is a regulated rural...

49 CFR 195.11 - What is a regulated rural gathering line and what requirements apply?

Code of Federal Regulations, 2010 CFR

2010-10-01

... subpart H of this part, except corrosion control is not required for pipelines existing on July 3, 2008... (Continued) PIPELINE AND HAZARDOUS MATERIALS SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) PIPELINE SAFETY TRANSPORTATION OF HAZARDOUS LIQUIDS BY PIPELINE General § 195.11 What is a regulated rural...

Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles.

PubMed

Kong, Dong; Farmer, Veronica; Shukla, Anil; James, Jana; Gruskin, Richard; Kiriyama, Shigeo; Loncarek, Jadranka

2014-09-29

Newly formed centrioles in cycling cells undergo a maturation process that is almost two cell cycles long before they become competent to function as microtubule-organizing centers and basal bodies. As a result, each cell contains three generations of centrioles, only one of which is able to form cilia. It is not known how this long and complex process is regulated. We show that controlled Plk1 activity is required for gradual biochemical and structural maturation of the centrioles and timely appendage assembly. Inhibition of Plk1 impeded accumulation of appendage proteins and appendage formation. Unscheduled Plk1 activity, either in cycling or interphase-arrested cells, accelerated centriole maturation and appendage and cilia formation on the nascent centrioles, erasing the age difference between centrioles in one cell. These findings provide a new understanding of how the centriole cycle is regulated and how proper cilia and centrosome numbers are maintained in the cells.

Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles

PubMed Central

Kong, Dong; Farmer, Veronica; Shukla, Anil; James, Jana; Gruskin, Richard; Kiriyama, Shigeo

2014-01-01

Newly formed centrioles in cycling cells undergo a maturation process that is almost two cell cycles long before they become competent to function as microtubule-organizing centers and basal bodies. As a result, each cell contains three generations of centrioles, only one of which is able to form cilia. It is not known how this long and complex process is regulated. We show that controlled Plk1 activity is required for gradual biochemical and structural maturation of the centrioles and timely appendage assembly. Inhibition of Plk1 impeded accumulation of appendage proteins and appendage formation. Unscheduled Plk1 activity, either in cycling or interphase-arrested cells, accelerated centriole maturation and appendage and cilia formation on the nascent centrioles, erasing the age difference between centrioles in one cell. These findings provide a new understanding of how the centriole cycle is regulated and how proper cilia and centrosome numbers are maintained in the cells. PMID:25246616

Fine regulation of RhoA and Rock is required for skeletal muscle differentiation.

PubMed

Castellani, Loriana; Salvati, Erica; Alemà, Stefano; Falcone, Germana

2006-06-02

The RhoA GTPase controls a variety of cell functions such as cell motility, cell growth, and gene expression. Previous studies suggested that RhoA mediates signaling inputs that promote skeletal myogenic differentiation. We show here that levels and activity of RhoA protein are down-regulated in both primary avian myoblasts and mouse satellite cells undergoing differentiation, suggesting that a fine regulation of this GTPase is required. In addition, ectopic expression of activated RhoA in primary quail myocytes, but not in mouse myocytes, inhibits accumulation of muscle-specific proteins and cell fusion. By disrupting RhoA signaling with specific inhibitors, we have shown that this GTPase, although required for cell identity in proliferating myoblasts, is not essential for commitment to terminal differentiation and muscle gene expression. Ectopic expression of an activated form of its downstream effector, Rock, impairs differentiation of both avian and mouse myoblasts. Conversely, Rock inhibition with specific inhibitors and small interfering RNA-mediated gene silencing leads to accelerated progression in the lineage and enhanced cell fusion, underscoring a negative regulatory function of Rock in myogenesis. Finally, we have reported that Rock acts independently from RhoA in preventing myoblast exit from the cell cycle and commitment to differentiation and may receive signaling inputs from Raf-1 kinase.

MGOUN1 encodes an Arabidopsis type IB DNA topoisomerase required in stem cell regulation and to maintain developmentally regulated gene silencing.

PubMed

Graf, Philipp; Dolzblasz, Alicja; Würschum, Tobias; Lenhard, Michael; Pfreundt, Ulrike; Laux, Thomas

2010-03-01

Maintenance of stem cells in the Arabidopsis thaliana shoot meristem is regulated by signals from the underlying cells of the organizing center, provided through the transcription factor WUSCHEL (WUS). Here, we report the isolation of several independent mutants of MGOUN1 (MGO1) as genetic suppressors of ectopic WUS activity and enhancers of stem cell defects in hypomorphic wus alleles. mgo1 mutants have previously been reported to result in a delayed progression of meristem cells into differentiating organ primordia (Laufs et al., 1998). Genetic analyses indicate that MGO1 functions together with WUS in stem cell maintenance at all stages of shoot and floral meristems. Synergistic interactions of mgo1 with several chromatin mutants suggest that MGO1 affects gene expression together with chromatin remodeling pathways. In addition, the expression states of developmentally regulated genes are randomly switched in mgo1 in a mitotically inheritable way, indicating that MGO1 stabilizes epigenetic states against stochastically occurring changes. Positional cloning revealed that MGO1 encodes a putative type IB topoisomerase, which in animals and yeast has been shown to be required for regulation of DNA coiling during transcription and replication. The specific developmental defects in mgo1 mutants link topoisomerase IB function in Arabidopsis to stable propagation of developmentally regulated gene expression.

Overview of existing regulations for ventilation requirements of enclosed vehicular parking facilities

DOE Office of Scientific and Technical Information (OSTI.GOV)

Krarti, M.; Ayari, A.M.

1999-07-01

This paper provides an overview of the current standards and regulations regarding the ventilation in enclosed parking facilities in the US and other countries. First, the paper discusses the emission rates of motor vehicle pollutants and their health effects. In particular, typical emission rates for different vehicle and fuel types are presented to highlight the effect of various parameters on the ventilation rate requirements for parking garages. In addition, the paper provides a brief description of some of the common ventilation problems reported in the literature for enclosed parking garages.

Profiles of Motivated Self-Regulation in College Computer Science Courses: Differences in Major versus Required Non-Major Courses

ERIC Educational Resources Information Center

Shell, Duane F.; Soh, Leen-Kiat

2013-01-01

The goal of the present study was to utilize a profiling approach to understand differences in motivation and strategic self-regulation among post-secondary STEM students in major versus required non-major computer science courses. Participants were 233 students from required introductory computer science courses (194 men; 35 women; 4 unknown) at…

Profiles of Motivated Self-Regulation in College Computer Science Courses: Differences in Major versus Required Non-Major Courses

NASA Astrophysics Data System (ADS)

Shell, Duane F.; Soh, Leen-Kiat

2013-12-01

The goal of the present study was to utilize a profiling approach to understand differences in motivation and strategic self-regulation among post-secondary STEM students in major versus required non-major computer science courses. Participants were 233 students from required introductory computer science courses (194 men; 35 women; 4 unknown) at a large Midwestern state university. Cluster analysis identified five profiles: (1) a strategic profile of a highly motivated by-any-means good strategy user; (2) a knowledge-building profile of an intrinsically motivated autonomous, mastery-oriented student; (3) a surface learning profile of a utility motivated minimally engaged student; (4) an apathetic profile of an amotivational disengaged student; and (5) a learned helpless profile of a motivated but unable to effectively self-regulate student. Among CS majors and students in courses in their major field, the strategic and knowledge-building profiles were the most prevalent. Among non-CS majors and students in required non-major courses, the learned helpless, surface learning, and apathetic profiles were the most prevalent. Students in the strategic and knowledge-building profiles had significantly higher retention of computational thinking knowledge than students in other profiles. Students in the apathetic and surface learning profiles saw little instrumentality of the course for their future academic and career objectives. Findings show that students in STEM fields taking required computer science courses exhibit the same constellation of motivated strategic self-regulation profiles found in other post-secondary and K-12 settings.

48 CFR 6.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Requirement. 6.501 Section 6.501 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 6.501 Requirement. As required by section 20 of the Office of...

48 CFR 6.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Requirement. 6.501 Section 6.501 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 6.501 Requirement. As required by section 20 of the Office of...

48 CFR 6.501 - Requirement.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Requirement. 6.501 Section 6.501 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 6.501 Requirement. As required by section 20 of the Office of...

Iowa SIP: Chapter 33 - Special Regulations and Construction Permit Requirements for Major Stationary Sources - Prevention of Significant Deterioration (PSD) of Air Quality

EPA Pesticide Factsheets

EPA Approved Iowa Regulations - 40 CFR 52.820(c) Chapter 33 - Special Regulations and Construction Permit Requirements for Major Stationary Sources - Prevention of Significant Deterioration (PSD) of Air Quality

48 CFR 6.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Requirement. 6.501 Section 6.501 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Advocates for Competition 6.501 Requirement. As required by 41 U.S.C. 1705, the head...

Hepatic Mitochondrial Pyruvate Carrier 1 Is Required for Efficient Regulation of Gluconeogenesis and Whole-Body Glucose Homeostasis.

PubMed

Gray, Lawrence R; Sultana, Mst Rasheda; Rauckhorst, Adam J; Oonthonpan, Lalita; Tompkins, Sean C; Sharma, Arpit; Fu, Xiaorong; Miao, Ren; Pewa, Alvin D; Brown, Kathryn S; Lane, Erin E; Dohlman, Ashley; Zepeda-Orozco, Diana; Xie, Jianxin; Rutter, Jared; Norris, Andrew W; Cox, James E; Burgess, Shawn C; Potthoff, Matthew J; Taylor, Eric B

2015-10-06

Gluconeogenesis is critical for maintenance of euglycemia during fasting. Elevated gluconeogenesis during type 2 diabetes (T2D) contributes to chronic hyperglycemia. Pyruvate is a major gluconeogenic substrate and requires import into the mitochondrial matrix for channeling into gluconeogenesis. Here, we demonstrate that the mitochondrial pyruvate carrier (MPC) comprising the Mpc1 and Mpc2 proteins is required for efficient regulation of hepatic gluconeogenesis. Liver-specific deletion of Mpc1 abolished hepatic MPC activity and markedly decreased pyruvate-driven gluconeogenesis and TCA cycle flux. Loss of MPC activity induced adaptive utilization of glutamine and increased urea cycle activity. Diet-induced obesity increased hepatic MPC expression and activity. Constitutive Mpc1 deletion attenuated the development of hyperglycemia induced by a high-fat diet. Acute, virally mediated Mpc1 deletion after diet-induced obesity decreased hyperglycemia and improved glucose tolerance. We conclude that the MPC is required for efficient regulation of gluconeogenesis and that the MPC contributes to the elevated gluconeogenesis and hyperglycemia in T2D. Copyright © 2015 Elsevier Inc. All rights reserved.

The regulation of vascular endothelial growth factor-induced microvascular permeability requires Rac and reactive oxygen species.

PubMed

Monaghan-Benson, Elizabeth; Burridge, Keith

2009-09-18

Vascular permeability is a complex process involving the coordinated regulation of multiple signaling pathways in the endothelial cell. It has long been documented that vascular endothelial growth factor (VEGF) greatly enhances microvascular permeability; however, the molecular mechanisms controlling VEGF-induced permeability remain unknown. Treatment of microvascular endothelial cells with VEGF led to an increase in reactive oxygen species (ROS) production. ROS are required for VEGF-induced permeability as treatment with the free radical scavenger, N-acetylcysteine, inhibited this effect. Additionally, treatment with VEGF caused ROS-dependent tyrosine phosphorylation of both vascular-endothelial (VE)-cadherin and beta-catenin. Rac1 was required for the VEGF-induced increase in permeability and adherens junction protein phosphorylation. Knockdown of Rac1 inhibited VEGF-induced ROS production consistent with Rac lying upstream of ROS in this pathway. Collectively, these data suggest that VEGF leads to a Rac-mediated generation of ROS, which, in turn, elevates the tyrosine phosphorylation of VE-cadherin and beta-catenin, ultimately regulating adherens junction integrity.

translin Is Required for Metabolic Regulation of Sleep.

PubMed

Murakami, Kazuma; Yurgel, Maria E; Stahl, Bethany A; Masek, Pavel; Mehta, Aradhana; Heidker, Rebecca; Bollinger, Wesley; Gingras, Robert M; Kim, Young-Joon; Ja, William W; Suter, Beat; DiAngelo, Justin R; Keene, Alex C

2016-04-04

Dysregulation of sleep or feeding has enormous health consequences. In humans, acute sleep loss is associated with increased appetite and insulin insensitivity, while chronically sleep-deprived individuals are more likely to develop obesity, metabolic syndrome, type II diabetes, and cardiovascular disease. Conversely, metabolic state potently modulates sleep and circadian behavior; yet, the molecular basis for sleep-metabolism interactions remains poorly understood. Here, we describe the identification of translin (trsn), a highly conserved RNA/DNA binding protein, as essential for starvation-induced sleep suppression. Strikingly, trsn does not appear to regulate energy stores, free glucose levels, or feeding behavior suggesting the sleep phenotype of trsn mutant flies is not a consequence of general metabolic dysfunction or blunted response to starvation. While broadly expressed in all neurons, trsn is transcriptionally upregulated in the heads of flies in response to starvation. Spatially restricted rescue or targeted knockdown localizes trsn function to neurons that produce the tachykinin family neuropeptide Leucokinin. Manipulation of neural activity in Leucokinin neurons revealed these neurons to be required for starvation-induced sleep suppression. Taken together, these findings establish trsn as an essential integrator of sleep and metabolic state, with implications for understanding the neural mechanism underlying sleep disruption in response to environmental perturbation. Copyright © 2016 Elsevier Ltd. All rights reserved.

TMEM203 Is a Novel Regulator of Intracellular Calcium Homeostasis and Is Required for Spermatogenesis

PubMed Central

Shambharkar, Prashant B.; Bittinger, Mark; Latario, Brian; Xiong, ZhaoHui; Bandyopadhyay, Somnath; Davis, Vanessa; Lin, Victor; Yang, Yi; Valdez, Reginald; Labow, Mark A.

2015-01-01

Intracellular calcium signaling is critical for initiating and sustaining diverse cellular functions including transcription, synaptic signaling, muscle contraction, apoptosis and fertilization. Trans-membrane 203 (TMEM203) was identified here in cDNA overexpression screens for proteins capable of modulating intracellular calcium levels using activation of a calcium/calcineurin regulated transcription factor as an indicator. Overexpression of TMEM203 resulted in a reduction of Endoplasmic Reticulum (ER) calcium stores and elevation in basal cytoplasmic calcium levels. TMEM203 protein was localized to the ER and found associated with a number of ER proteins which regulate ER calcium entry and efflux. Mouse Embryonic Fibroblasts (MEFs) derived from Tmem203 deficient mice had reduced ER calcium stores and altered calcium homeostasis. Tmem203 deficient mice were viable though male knockout mice were infertile and exhibited a severe block in spermiogenesis and spermiation. Expression profiling studies showed significant alternations in expression of calcium channels and pumps in testes and concurrently Tmem203 deficient spermatocytes demonstrated significantly altered calcium handling. Thus Tmem203 is an evolutionarily conserved regulator of cellular calcium homeostasis, is required for spermatogenesis and provides a causal link between intracellular calcium regulation and spermiogenesis. PMID:25996873

Experiencing reproductive concerns as a female cancer survivor is associated with depression

PubMed Central

Gorman, Jessica R.; Su, H. Irene; Roberts, Samantha C.; Dominick, Sally A.; Malcarne, Vanessa L.

2014-01-01

Background Young adult female cancer survivors have unmet reproductive concerns and informational needs that are associated with poorer quality of life. The purpose of this study was to examine the association between current reproductive concerns and moderate to severe depression among young survivors. Methods This cross-sectional study includes 200 female cancer survivors between the ages of 18 and 35 years who completed a web-based survey measuring reproductive history, parenthood desires, reproductive concerns after cancer, and quality of life indicators. Results The mean age of participants was 28 years (SD = 4.4) and almost two-thirds were diagnosed within 5 years of completing the survey. Multivariable logistic regression analysis controlling for education, duration of survivorship, and social support revealed an association between experiencing reproductive concerns and moderate to severe depression (OR = 1.30, 95% CI = 1.06–1.60 for each 5 unit increase in RCAC score). Of those with moderate to severe depression, 23% had high RCAC scores as compared to 6% of those with minimal to mild depression (p < 0.001). Conclusion A higher level of reproductive concerns was associated with greater odds of experiencing moderate to severe depression. Almost a quarter of survivors in this sample reported moderate to severe depression, and addressing reproductive concerns represents one potential area of intervention to improve the psychosocial health of young survivors. PMID:25377593

48 CFR 31.703 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Requirements. 31.703 Section 31.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 31.703 Requirements...

48 CFR 3406.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Requirement. 3406.501 Section 3406.501 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3406.501 Requirement. The...

48 CFR 31.703 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Requirements. 31.703 Section 31.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 31.703 Requirements...

48 CFR 3406.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Requirement. 3406.501 Section 3406.501 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3406.501 Requirement. The...

48 CFR 31.703 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Requirements. 31.703 Section 31.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 31.703 Requirements...

48 CFR 31.703 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Requirements. 31.703 Section 31.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 31.703 Requirements...

48 CFR 3406.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Requirement. 3406.501 Section 3406.501 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3406.501 Requirement. The...

Plasma membrane H+-ATPase regulation is required for auxin gradient formation preceding phototropic growth

PubMed Central

Hohm, Tim; Demarsy, Emilie; Quan, Clément; Allenbach Petrolati, Laure; Preuten, Tobias; Vernoux, Teva; Bergmann, Sven; Fankhauser, Christian

2014-01-01

Phototropism is a growth response allowing plants to align their photosynthetic organs toward incoming light and thereby to optimize photosynthetic activity. Formation of a lateral gradient of the phytohormone auxin is a key step to trigger asymmetric growth of the shoot leading to phototropic reorientation. To identify important regulators of auxin gradient formation, we developed an auxin flux model that enabled us to test in silico the impact of different morphological and biophysical parameters on gradient formation, including the contribution of the extracellular space (cell wall) or apoplast. Our model indicates that cell size, cell distributions, and apoplast thickness are all important factors affecting gradient formation. Among all tested variables, regulation of apoplastic pH was the most important to enable the formation of a lateral auxin gradient. To test this prediction, we interfered with the activity of plasma membrane H+-ATPases that are required to control apoplastic pH. Our results show that H+-ATPases are indeed important for the establishment of a lateral auxin gradient and phototropism. Moreover, we show that during phototropism, H+-ATPase activity is regulated by the phototropin photoreceptors, providing a mechanism by which light influences apoplastic pH. PMID:25261457

[Good for your health? An analysis of the requirements for scientific substantiation in European health claims regulation].

PubMed

Todt, Oliver; Luján, José Luis

2016-06-01

To identify the various types of evidence, as well as their relative importance in European health claims regulation, in order to analyze the consequences for consumer protection of the requirements for scientific substantiation in this regulation. Qualitative analysis of various documents relevant to the regulatory process, particularly as to the implications of the standards of proof for the functional food market, as well as consumer behavior. European regulation defines a hierarchy of evidence that turns randomized controlled trials into a necessary and sufficient condition for health claim authorizations. Consumer protection can be interpreted in different manners. High standards of proof protect consumers from false information about the health outcomes of functional foods, while lower standards lead to more, albeit less accurate information about such outcomes being available to consumers.

Sox9 regulates cell proliferation and is required for Paneth cell differentiation in the intestinal epithelium

PubMed Central

Bastide, Pauline; Darido, Charbel; Pannequin, Julie; Kist, Ralf; Robine, Sylvie; Marty-Double, Christiane; Bibeau, Frédéric; Scherer, Gerd; Joubert, Dominique; Hollande, Frédéric; Blache, Philippe; Jay, Philippe

2007-01-01

The HMG-box transcription factor Sox9 is expressed in the intestinal epithelium, specifically, in stem/progenitor cells and in Paneth cells. Sox9 expression requires an active β-catenin–Tcf complex, the transcriptional effector of the Wnt pathway. This pathway is critical for numerous aspects of the intestinal epithelium physiopathology, but processes that specify the cell response to such multipotential signals still remain to be identified. We inactivated the Sox9 gene in the intestinal epithelium to analyze its physiological function. Sox9 inactivation affected differentiation throughout the intestinal epithelium, with a disappearance of Paneth cells and a decrease of the goblet cell lineage. Additionally, the morphology of the colon epithelium was severely altered. We detected general hyperplasia and local crypt dysplasia in the intestine, and Wnt pathway target genes were up-regulated. These results highlight the central position of Sox9 as both a transcriptional target and a regulator of the Wnt pathway in the regulation of intestinal epithelium homeostasis. PMID:17698607

Gravin regulates mesodermal cell behavior changes required for axis elongation during zebrafish gastrulation.

PubMed

Weiser, Douglas C; Pyati, Ujwal J; Kimelman, David

2007-06-15

Convergent extension of the mesoderm is the major driving force of vertebrate gastrulation. During this process, mesodermal cells move toward the future dorsal side of the embryo, then radically change behavior as they initiate extension of the body axis. How cells make this transition in behavior is unknown. We have identified the scaffolding protein and tumor suppressor Gravin as a key regulator of this process in zebrafish embryos. We show that Gravin is required for the conversion of mesodermal cells from a highly migratory behavior to the medio-laterally intercalative behavior required for body axis extension. In the absence of Gravin, paraxial mesodermal cells fail to shut down the protrusive activity mediated by the Rho/ROCK/Myosin II pathway, resulting in embryos with severe extension defects. We propose that Gravin functions as an essential scaffold for regulatory proteins that suppress the migratory behavior of the mesoderm during gastrulation, and suggest that this function also explains how Gravin inhibits invasive behaviors in metastatic cells.

48 CFR 30.202 - Disclosure requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Disclosure requirements. 30.202 Section 30.202 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 30.202 Disclosure...

48 CFR 30.202 - Disclosure requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Disclosure requirements. 30.202 Section 30.202 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 30.202 Disclosure...

48 CFR 30.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Contract requirements. 30.201 Section 30.201 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 30.201 Contract...

Interpretation of Section 52.21(i) (3) of the Regulations for the PSD Requirement

EPA Pesticide Factsheets

This document may be of assistance in applying the New Source Review (NSR) air permitting regulations including the Prevention of Significant Deterioration (PSD) requirements. This document is part of the NSR Policy and Guidance Database. Some documents in the database are a scanned or retyped version of a paper photocopy of the original. Although we have taken considerable effort to quality assure the documents, some may contain typographical errors. Contact the office that issued the document if you need a copy of the original.

48 CFR 8.1103 - Contract requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Contract requirements. 8.1103 Section 8.1103 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Leasing of Motor Vehicles 8.1103 Contract requirements...

48 CFR 203.1003 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Requirements. 203.1003 Section 203.1003 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Business Ethics and Conduct 203.1003 Requirements. (b) Notification of possible contractor violation. Upon...

48 CFR 44.302 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Requirements. 44.302 Section 44.302 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractors' Purchasing Systems Reviews 44.302 Requirements. (a) The ACO...

48 CFR 203.1003 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 203.1003 Section 203.1003 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Business Ethics and Conduct 203.1003 Requirements. (b) Notification of possible contractor violation. Upon...

48 CFR 203.1003 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Requirements. 203.1003 Section 203.1003 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT... Business Ethics and Conduct 203.1003 Requirements. (b) Notification of possible contractor violation. Upon...

48 CFR 44.302 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Requirements. 44.302 Section 44.302 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractors' Purchasing Systems Reviews 44.302 Requirements. (a) The ACO...

48 CFR 23.601 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Requirements. 23.601 Section 23.601 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC... DRUG-FREE WORKPLACE Notice of Radioactive Material 23.601 Requirements. (a) The clause at 52.223-7...

Experiencing reproductive concerns as a female cancer survivor is associated with depression.

PubMed

Gorman, Jessica R; Su, H Irene; Roberts, Samantha C; Dominick, Sally A; Malcarne, Vanessa L

2015-03-15

Young adult female cancer survivors have unmet reproductive concerns and informational needs that are associated with poorer quality of life. The purpose of this study was to examine the association between current reproductive concerns and moderate to severe depression among young survivors. This cross-sectional study included 200 female cancer survivors between the ages of 18 and 35 years who completed a Web-based survey measuring reproductive history, parenthood desires, reproductive concerns after cancer, and quality-of-life indicators. The mean age of the participants was 28 years (standard deviation, 4.4 years), and almost two-thirds were diagnosed within 5 years of survey completion. A multivariate logistic regression analysis controlling for education, duration of survivorship, and social support revealed an association between experiencing reproductive concerns and moderate to severe depression (odds ratio for each 5-unit increase in the Reproductive Concerns After Cancer [RCAC] score, 1.30; 95% confidence interval, 1.06-1.60). Among those with moderate to severe depression, 23% had high RCAC scores, whereas 6% of those with minimal to mild depression did (P < .001). A higher level of reproductive concerns was associated with greater odds of experiencing moderate to severe depression. Almost a quarter of survivors in this sample reported moderate to severe depression, and addressing reproductive concerns represents one potential area of intervention for improving the psychosocial health of young survivors. © 2014 American Cancer Society.

A Subset of Cholinergic Mushroom Body Neurons Requires Go Signaling to Regulate Sleep in Drosophila

PubMed Central

Yi, Wei; Zhang, Yunpeng; Tian, Yinjun; Guo, Jing; Li, Yan; Guo, Aike

2013-01-01

Study Objectives: Identifying the neurochemistry and neural circuitry of sleep regulation is critical for understanding sleep and various sleep disorders. Fruit flies display sleep-like behavior, sharing essential features with sleep of vertebrate. In the fruit fly's central brain, the mushroom body (MB) has been highlighted as a sleep center; however, its neurochemical nature remains unclear, and whether it promotes sleep or wake is still a topic of controversy. Design: We used a video recording system to accurately monitor the locomotor activity and sleep status. Gene expression was temporally and regionally manipulated by heat induction and the Gal4/UAS system. Measurements and Results: We found that expressing pertussis toxin (PTX) in the MB by c309-Gal4 to block Go activity led to unique sleep defects as dramatic sleep increase in daytime and fragmented sleep in nighttime. We narrowed down the c309-Gal4 expressing brain regions to the MB α/β core neurons that are responsible for the Go-mediated sleep effects. Using genetic tools of neurotransmitter-specific Gal80 and RNA interference approach to suppress acetylcholine signal, we demonstrated that these MB α/β core neurons were cholinergic and sleep-promoting neurons, supporting that Go mediates an inhibitory signal. Interestingly, we found that adjacent MB α/β neurons were also cholinergic but wake-promoting neurons, in which Go signal was also required. Conclusion: Our findings in fruit flies characterized a group of sleep-promoting neurons surrounded by a group of wake-promoting neurons. The two groups of neurons are both cholinergic and use Go inhibitory signal to regulate sleep. Citation: Yi W; Zhang Y; Tian Y; Guo J; Li Y; Guo A. A subset of cholinergic mushroom body neurons requires go signaling to regulate sleep in Drosophila. SLEEP 2013;36(12):1809-1821. PMID:24293755

48 CFR 806.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirement. 806.501 Section 806.501 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 806.501 Requirement. (a) The Associate...

48 CFR 2406.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Requirement. 2406.501 Section 2406.501 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2406.501 Requirement. The...

48 CFR 606.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirement. 606.501 Section 606.501 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 606.501 Requirement. (a) The Procurement Executive...

48 CFR 606.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirement. 606.501 Section 606.501 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 606.501 Requirement. (a) The Procurement Executive...

48 CFR 906.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirement. 906.501 Section 906.501 Federal Acquisition Regulations System DEPARTMENT OF ENERGY COMPETITION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 906.501 Requirement. The Secretary of Energy and...

48 CFR 2406.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Requirement. 2406.501 Section 2406.501 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2406.501 Requirement. The...

48 CFR 406.501 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirements. 406.501 Section 406.501 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 406.501 Requirements. (a) The Chief...

48 CFR 1306.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirement. 1306.501 Section 1306.501 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1306.501 Requirement. The designee authorized to...

48 CFR 1206.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirement. 1206.501 Section 1206.501 Federal Acquisition Regulations System DEPARTMENT OF TRANSPORTATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1206.501 Requirement. The DOT Senior Competition Advocate (SCA...

48 CFR 606.501 - Requirement.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirement. 606.501 Section 606.501 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 606.501 Requirement. (a) The Procurement Executive...

48 CFR 1206.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirement. 1206.501 Section 1206.501 Federal Acquisition Regulations System DEPARTMENT OF TRANSPORTATION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1206.501 Requirement. The DOT Senior Competition Advocate (SCA...

48 CFR 1306.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirement. 1306.501 Section 1306.501 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1306.501 Requirement. The designee authorized to...

48 CFR 806.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirement. 806.501 Section 806.501 Federal Acquisition Regulations System DEPARTMENT OF VETERANS AFFAIRS COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 806.501 Requirement. (a) The Associate...

48 CFR 406.501 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirements. 406.501 Section 406.501 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 406.501 Requirements. (a) The Chief...

48 CFR 906.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirement. 906.501 Section 906.501 Federal Acquisition Regulations System DEPARTMENT OF ENERGY COMPETITION ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 906.501 Requirement. The Secretary of Energy has...

48 CFR 406.501 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirements. 406.501 Section 406.501 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 406.501 Requirements. (a) The Chief...

48 CFR 406.501 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirements. 406.501 Section 406.501 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 406.501 Requirements. (a) The Chief...

48 CFR 1406.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirement. 1406.501 Section 1406.501 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1406.501 Requirement. (a) The competition...

48 CFR 606.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirement. 606.501 Section 606.501 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 606.501 Requirement. (a) The Procurement Executive...

48 CFR 406.501 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirements. 406.501 Section 406.501 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 406.501 Requirements. (a) The Chief...

48 CFR 2406.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Requirement. 2406.501 Section 2406.501 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2406.501 Requirement. The...

48 CFR 1406.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirement. 1406.501 Section 1406.501 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 1406.501 Requirement. (a) The competition...

48 CFR 2406.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Requirement. 2406.501 Section 2406.501 Federal Acquisition Regulations System DEPARTMENT OF HOUSING AND URBAN DEVELOPMENT COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2406.501 Requirement. The...

48 CFR 606.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirement. 606.501 Section 606.501 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 606.501 Requirement. (a) The Procurement Executive...

Identification of a DNA sequence motif required for expression of iron-regulated genes in pseudomonads.

PubMed

Rombel, I T; McMorran, B J; Lamont, I L

1995-02-20

Many bacteria respond to a lack of iron in the environment by synthesizing siderophores, which act as iron-scavenging compounds. Fluorescent pseudomonads synthesize strain-specific but chemically related siderophores called pyoverdines or pseudobactins. We have investigated the mechanisms by which iron controls expression of genes involved in pyoverdine metabolism in Pseudomonas aeruginosa. Transcription of these genes is repressed by the presence of iron in the growth medium. Three promoters from these genes were cloned and the activities of the promoters were dependent on the amounts of iron in the growth media. Two of the promoters were sequenced and the transcriptional start site were identified by S1 nuclease analysis. Sequences similar to the consensus binding site for the Fur repressor protein, which controls expression of iron-repressible genes in several gram-negative species, were not present in the promoters, suggesting that they are unlikely to have a high affinity for Fur. However, comparison of the promoter sequences with those of iron-regulated genes from other Pseudomonas species and also the iron-regulated exotoxin gene of P. aeruginosa allowed identification of a shared sequence element, with the consensus sequence (G/C)CTAAAT-CCC, which is likely to act as a binding site for a transcriptional activator protein. Mutations in this sequence greatly reduced the activities of the promoters characterized here as well as those of other iron-regulated promoters. The requirement for this motif in the promoters of iron-regulated genes of different Pseudomonas species indicates that similar mechanisms are likely to be involved in controlling expression of a range of iron-regulated genes in pseudomonads.

Calcium signaling in taste cells: regulation required.

PubMed

Medler, Kathryn F

2010-11-01

Peripheral taste receptor cells depend on distinct calcium signals to generate appropriate cellular responses that relay taste information to the central nervous system. Some taste cells have conventional chemical synapses and rely on calcium influx through voltage-gated calcium channels. Other taste cells lack these synapses and depend on calcium release from stores to formulate an output signal through a hemichannel. Despite the importance of calcium signaling in taste cells, little is known about how these signals are regulated. This review summarizes recent studies that have identified 2 calcium clearance mechanisms expressed in taste cells, including mitochondrial calcium uptake and sodium/calcium exchangers (NCXs). These studies identified a unique constitutive calcium influx that contributes to maintaining appropriate calcium homeostasis in taste cells and the role of the mitochondria and exchangers in this process. The additional role of NCXs in the regulation of evoked calcium responses is also discussed. Clearly, calcium signaling is a dynamic process in taste cells and appears to be more complex than has previously been appreciated.

Successful emotion regulation requires both conviction and skill: beliefs about the controllability of emotions, reappraisal, and regulation success.

PubMed

Gutentag, Tony; Halperin, Eran; Porat, Roni; Bigman, Yochanan E; Tamir, Maya

2017-09-01

To succeed in self-regulation, people need to believe that it is possible to change behaviour and they also need to use effective means to enable such a change. We propose that this also applies to emotion regulation. In two studies, we found that people were most successful in emotion regulation, the more they believed emotions can be controlled and the more they used an effective emotion regulation strategy - namely, cognitive reappraisal. Cognitive reappraisal moderated the link between beliefs about the controllability of emotion and success in emotion regulation, when reappraisal was measured as a trait (Study 1) or manipulated (Study 2). Such moderation was found when examining the regulation of disgust elicited by emotion-inducing films (Study 1), and the regulation of anger elicited by real political events (Study 2). We discuss the implications of our findings for research and practice in emotion regulation.

48 CFR 22.1002 - Statutory requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Statutory requirements. 22.1002 Section 22.1002 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Amended 22.1002 Statutory requirements. ...

48 CFR 306.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirement. 306.501 Section 306.501 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 306.501 Requirement. The HHS CA is the...

48 CFR 306.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirement. 306.501 Section 306.501 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 306.501 Requirement. The HHS CA is the...

48 CFR 3006.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Requirement. 3006.501... ACQUISITION REGULATION (HSAR) ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3006.501 Requirement. The DHS Senior Competition Advocate (SCA) is located in the Office of the Chief Procurement...

48 CFR 3006.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Requirement. 3006.501... ACQUISITION REGULATION (HSAR) ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3006.501 Requirement. The DHS Senior Competition Advocate (SCA) is located in the Office of the Chief Procurement...

48 CFR 2806.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Requirement. 2806.501 Section 2806.501 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2806.501 Requirement. In accordance with FAR 6.501...

48 CFR 3003.1003 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Requirements. 3003.1003 Section 3003.1003 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND... INTEREST Contractor Code of Business Ethics and Conduct 3003.1003 Requirements. (a) Contractor requirements...

48 CFR 2806.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Requirement. 2806.501 Section 2806.501 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2806.501 Requirement. In accordance with FAR 6.501...

48 CFR 306.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirement. 306.501 Section 306.501 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 306.501 Requirement. The HHS CA is the...

48 CFR 3006.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Requirement. 3006.501... ACQUISITION REGULATION (HSAR) ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3006.501 Requirement. The DHS Senior Competition Advocate (SCA) is located in the Office of the Chief Procurement...

48 CFR 3003.1003 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Requirements. 3003.1003 Section 3003.1003 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND... INTEREST Contractor Code of Business Ethics and Conduct 3003.1003 Requirements. (a) Contractor requirements...

48 CFR 2806.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Requirement. 2806.501 Section 2806.501 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 2806.501 Requirement. In accordance with FAR 6.501...

48 CFR 3003.1003 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Requirements. 3003.1003 Section 3003.1003 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND... INTEREST Contractor Code of Business Ethics and Conduct 3003.1003 Requirements. (a) Contractor requirements...

48 CFR 3006.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Requirement. 3006.501... ACQUISITION REGULATION (HSAR) ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 3006.501 Requirement. The DHS Senior Competition Advocate (SCA) is located in the Office of the Chief Procurement...

75 FR 26121 - Regulation of Fuels and Fuel Additives: Alternative Affirmative Defense Requirements for Ultra...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-05-11

...EPA is issuing a direct final rule to amend the diesel sulfur regulations to allow refiners, importers, distributors, and retailers of highway diesel fuel the option to use an alternative affirmative defense if the Agency finds highway diesel fuel samples above the specified sulfur standard at retail facilities. This alternative defense consists of a comprehensive program of quality assurance sampling and testing that would cover all participating companies that produce and/or distribute highway diesel fuel if certain other conditions are met. The sampling and testing program would be carried out by an independent surveyor. The program would be conducted pursuant to a survey plan approved by EPA that is designed to achieve the same objectives as the current regulatory quality assurance requirement. This rule also amends the gasoline benzene regulations to allow disqualified small refiners the same opportunity to generate gasoline benzene credits as that afforded to non-small refiners.

Establishment of a dental license regulation authority is required in Korea: results of the Delphi technique.

PubMed

Choi, Jin-Woo; Kim, Kack-Kyun; Lee, Jihyun; Choi, Dong-Ju; Kim, Kyung-Nyun

2017-01-01

In addition to dental education, a system for the evaluation and management of dental licensing and certification is required to meet the growing societal demand for more competent dentists. In this study, the Delphi technique was used to gather opinions from a variety of professionals on the problems of and remedies for the dental license management system in Korea. Delphi surveys were conducted from April 2016 to October 2016 in South Korea. A variety of dental professionals were included and categorized into 3 groups according to their expertise as follows: the basic dentistry group, the clinical dentistry group, and the policy group. The Delphi technique was conducted in 3 rounds of e-mail surveys, each with different questions that probed with increasing depth on the dental license management system. In each successive round, the responses were categorized, scored on a Likert scale, and statistically analyzed. After categorizing the results of the first survey and ranking the results of the second survey using the Delphi technique, regulation by a licensing authority was found to be the most critical issue. This was followed by the license renewal system, continuing education, a tiered licensure system, improvement of foreign license approval, and utilization of retirees, in decreasing order of importance. The third Delphi survey showed a similar ranking, with regulation by a licensing authority being the major concern. Opinions regarding the dental license management system were provided as open-ended responses. The responses of the 3 groups showed statistically significant differences in the scores for the issue of regulation by a licensing authority. After re-grouping into the dentistry group and the policy group, the issue received a significantly higher score in the dentistry group. The quality of dental treatment should be managed to protect patients and dental professionals. For this purpose, the establishment of an independent license regulation

48 CFR 630.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Contract requirements. 630.201 Section 630.201 Federal Acquisition Regulations System DEPARTMENT OF STATE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 630.201 Contract requirements. ...

48 CFR 330.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Contract requirements. 330.201 Section 330.201 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS CAS Program Requirements 330.201 Contract requirements. ...

Distinct requirements of wls, wnt9a, wnt5b and gpc4 in regulating chondrocyte maturation and timing of endochondral ossification

PubMed Central

Ling, Irving TC; Rochard, Lucie; Liao, Eric C.

2017-01-01

Formation of the mandible requires progressive morphologic change, proliferation, differentiation and organization of chondrocytes preceding osteogenesis. The Wnt signaling pathway is involved in regulating bone development and maintenance. Chondrocytes that are fated to become bone require Wnt to polarize and orientate appropriately to initiate the endochondral ossification program. Although the canonical Wnt signaling has been well studied in the context of bone development, the effects of non-canonical Wnt signaling in regulating the timing of cartilage maturation and subsequent bone formation in shaping ventral craniofacial structure is not fully understood.. Here we examined the role of the non-canonical Wnt signaling pathway (wls, gpc4, wnt5b and wnt9a) in regulating zebrafish Meckel’s cartilage maturation to the onset of osteogenic differentiation. We found that disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. This study demonstrates the importance of the non-canonical Wnt pathway in regulating coordinated ventral cartilage morphogenesis and ossification. PMID:27908786

48 CFR 32.703 - Contract funding requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Contract funding requirements. 32.703 Section 32.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 32.703 Contract funding requirements. ...

48 CFR 32.703 - Contract funding requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Contract funding requirements. 32.703 Section 32.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 32.703 Contract funding requirements. ...

48 CFR 32.703 - Contract funding requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Contract funding requirements. 32.703 Section 32.703 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 32.703 Contract funding requirements. ...

Plasma membrane H⁺ -ATPase regulation is required for auxin gradient formation preceding phototropic growth.

PubMed

Hohm, Tim; Demarsy, Emilie; Quan, Clément; Allenbach Petrolati, Laure; Preuten, Tobias; Vernoux, Teva; Bergmann, Sven; Fankhauser, Christian

2014-09-26

Phototropism is a growth response allowing plants to align their photosynthetic organs toward incoming light and thereby to optimize photosynthetic activity. Formation of a lateral gradient of the phytohormone auxin is a key step to trigger asymmetric growth of the shoot leading to phototropic reorientation. To identify important regulators of auxin gradient formation, we developed an auxin flux model that enabled us to test in silico the impact of different morphological and biophysical parameters on gradient formation, including the contribution of the extracellular space (cell wall) or apoplast. Our model indicates that cell size, cell distributions, and apoplast thickness are all important factors affecting gradient formation. Among all tested variables, regulation of apoplastic pH was the most important to enable the formation of a lateral auxin gradient. To test this prediction, we interfered with the activity of plasma membrane H⁺ -ATPases that are required to control apoplastic pH. Our results show that H⁺ -ATPases are indeed important for the establishment of a lateral auxin gradient and phototropism. Moreover, we show that during phototropism, H⁺ -ATPase activity is regulated by the phototropin photoreceptors, providing a mechanism by which light influences apoplastic pH. © 2014 The Authors. Published under the terms of the CC BY 4.0 license.

48 CFR 30.202-1 - General requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false General requirements. 30.202-1 Section 30.202-1 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 30.202-1 General...

The Ustilago maydis Nit2 Homolog Regulates Nitrogen Utilization and Is Required for Efficient Induction of Filamentous Growth

PubMed Central

Horst, Robin J.; Zeh, Christine; Saur, Alexandra; Sonnewald, Sophia; Sonnewald, Uwe

2012-01-01

Nitrogen catabolite repression (NCR) is a regulatory strategy found in microorganisms that restricts the utilization of complex and unfavored nitrogen sources in the presence of favored nitrogen sources. In fungi, this concept has been best studied in yeasts and filamentous ascomycetes, where the GATA transcription factors Gln3p and Gat1p (in yeasts) and Nit2/AreA (in ascomycetes) constitute the main positive regulators of NCR. The reason why functional Nit2 homologs of some phytopathogenic fungi are required for full virulence in their hosts has remained elusive. We have identified the Nit2 homolog in the basidiomycetous phytopathogen Ustilago maydis and show that it is a major, but not the exclusive, positive regulator of nitrogen utilization. By transcriptome analysis of sporidia grown on artificial media devoid of favored nitrogen sources, we show that only a subset of nitrogen-responsive genes are regulated by Nit2, including the Gal4-like transcription factor Ton1 (a target of Nit2). Ustilagic acid biosynthesis is not under the control of Nit2, while nitrogen starvation-induced filamentous growth is largely dependent on functional Nit2. nit2 deletion mutants show the delayed initiation of filamentous growth on maize leaves and exhibit strongly compromised virulence, demonstrating that Nit2 is required to efficiently initiate the pathogenicity program of U. maydis. PMID:22247264

48 CFR 3030.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 7 2011-10-01 2011-10-01 false Contract requirements. 3030.201 Section 3030.201 Federal Acquisition Regulations System DEPARTMENT OF HOMELAND SECURITY, HOMELAND SECURITY ACQUISITION REGULATION (HSAR) GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS...

The rgg0182 gene encodes a transcriptional regulator required for the full Streptococcus thermophilus LMG18311 thermal adaptation.

PubMed

Henry, Romain; Bruneau, Emmanuelle; Gardan, Rozenn; Bertin, Stéphane; Fleuchot, Betty; Decaris, Bernard; Leblond-Bourget, Nathalie

2011-10-07

Streptococcus thermophilus is an important starter strain for the production of yogurt and cheeses. The analysis of sequenced genomes of four strains of S. thermophilus indicates that they contain several genes of the rgg familly potentially encoding transcriptional regulators. Some of the Rgg proteins are known to be involved in bacterial stress adaptation. In this study, we demonstrated that Streptococcus thermophilus thermal stress adaptation required the rgg0182 gene which transcription depends on the culture medium and the growth temperature. This gene encoded a protein showing similarity with members of the Rgg family transcriptional regulator. Our data confirmed that Rgg0182 is a transcriptional regulator controlling the expression of its neighboring genes as well as chaperones and proteases encoding genes. Therefore, analysis of a Δrgg0182 mutant revealed that this protein played a role in the heat shock adaptation of Streptococcus thermophilus LMG18311. These data showed the importance of the Rgg0182 transcriptional regulator on the survival of S. thermophilus during dairy processes and more specifically during changes in temperature.

Design for pressure regulating components

NASA Technical Reports Server (NTRS)

Wichmann, H.

1973-01-01

The design development for Pressure Regulating Components included a regulator component trade-off study with analog computer performance verification to arrive at a final optimized regulator configuration for the Space Storable Propulsion Module, under development for a Jupiter Orbiter mission. This application requires the pressure regulator to be capable of long-term fluorine exposure. In addition, individual but basically identical (for purposes of commonality) units are required for separate oxidizer and fuel pressurization. The need for dual units requires improvement in the regulation accuracy over present designs. An advanced regulator concept was prepared featuring redundant bellows, all metallic/ceramic construction, friction-free guidance of moving parts, gas damping, and the elimination of coil springs normally used for reference forces. The activities included testing of actual size seat/poppet components to determine actual discharge coefficients and flow forces. The resulting data was inserted into the computer model of the regulator. Computer simulation of the propulsion module performance over two mission profiles indicated satisfactory minimization of propellant residual requirements imposed by regulator performance uncertainties.

40 CFR 141.700 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Treatment for Cryptosporidium General... drinking water regulations. The regulations in this subpart establish or extend treatment technique... requirements of this subpart for filtered systems apply to systems required by National Primary Drinking Water...

40 CFR 141.70 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

...) NATIONAL PRIMARY DRINKING WATER REGULATIONS Filtration and Disinfection § 141.70 General requirements. (a) The requirements of this subpart H constitute national primary drinking water regulations. These... filtration requirements in § 141.73 and the disinfection requirements in § 141.72(b). (c) Each public water...

48 CFR 9903.202 - Disclosure requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... ACCOUNTING STANDARDS CONTRACT COVERAGE CAS Program Requirements 9903.202 Disclosure requirements. ... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Disclosure requirements. 9903.202 Section 9903.202 Federal Acquisition Regulations System COST ACCOUNTING STANDARDS BOARD...

Iron-regulated biofilm formation in Staphylococcus aureus Newman requires ica and the secreted protein Emp.

PubMed

Johnson, Miranda; Cockayne, Alan; Morrissey, Julie A

2008-04-01

Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface and have previously been implicated in a number of aspects of S. aureus pathogenesis. We showed here that the transcription of these important virulence factors is induced by growth in low-iron medium, reflective of the in vivo environment. Our results show that iron regulation of Eap and Emp is Fur independent. However, Fur is required for full induction of eap and emp expression in low-iron conditions. In this study, we demonstrated that in addition to Fur, low-iron-induced biofilm formation requires Sae, Agr, and SarA. In iron-restricted growth conditions, Sae and Agr are essential for Emp and Eap expression and hence for biofilm formation, whereas SarA appears to have a less-significant role. We also showed that expression of the ica operon is required for biofilm formation in iron-restricted growth conditions. We demonstrated that in fact, ica is required for the expression of the important multifunctional virulence determinants eap and emp.

Iron-Regulated Biofilm Formation in Staphylococcus aureus Newman Requires ica and the Secreted Protein Emp▿

PubMed Central

Johnson, Miranda; Cockayne, Alan; Morrissey, Julie A.

2008-01-01

Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface and have previously been implicated in a number of aspects of S. aureus pathogenesis. We showed here that the transcription of these important virulence factors is induced by growth in low-iron medium, reflective of the in vivo environment. Our results show that iron regulation of Eap and Emp is Fur independent. However, Fur is required for full induction of eap and emp expression in low-iron conditions. In this study, we demonstrated that in addition to Fur, low-iron-induced biofilm formation requires Sae, Agr, and SarA. In iron-restricted growth conditions, Sae and Agr are essential for Emp and Eap expression and hence for biofilm formation, whereas SarA appears to have a less-significant role. We also showed that expression of the ica operon is required for biofilm formation in iron-restricted growth conditions. We demonstrated that in fact, ica is required for the expression of the important multifunctional virulence determinants eap and emp. PMID:18268030

48 CFR 1330.202 - Disclosure requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Disclosure requirements. 1330.202 Section 1330.202 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 1330.202 Disclosure...

48 CFR 1430.202 - Disclosure requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Disclosure requirements. 1430.202 Section 1430.202 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 1430.202 Disclosure...

48 CFR 430.202 - Disclosure requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Disclosure requirements. 430.202 Section 430.202 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 430.202 Disclosure...

40 CFR 141.130 - General requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Disinfectant Residuals, Disinfection Byproducts, and Disinfection Byproduct Precursors § 141.130 General requirements. (a) The requirements of this subpart L... treatment technique requirements for disinfection byproduct precursors in § 141.135. (2) The regulations in...

40 CFR 141.130 - General requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Disinfectant Residuals, Disinfection Byproducts, and Disinfection Byproduct Precursors § 141.130 General requirements. (a) The requirements of this subpart L... treatment technique requirements for disinfection byproduct precursors in § 141.135. (2) The regulations in...

48 CFR 1330.202 - Disclosure requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Disclosure requirements. 1330.202 Section 1330.202 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 1330.202 Disclosure...

48 CFR 1430.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Contract requirements. 1430.201 Section 1430.201 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 1430.201 Contract...

48 CFR 1430.202 - Disclosure requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Disclosure requirements. 1430.202 Section 1430.202 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 1430.202 Disclosure...

48 CFR 430.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Contract requirements. 430.201 Section 430.201 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 430.201 Contract...

48 CFR 430.202 - Disclosure requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Disclosure requirements. 430.202 Section 430.202 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 430.202 Disclosure...

48 CFR 1330.201 - Contract requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Contract requirements. 1330.201 Section 1330.201 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE GENERAL CONTRACTING REQUIREMENTS COST ACCOUNTING STANDARDS ADMINISTRATION CAS Program Requirements 1330.201 Contract...

48 CFR 252.247-7015 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 252.247-7015 Section 252.247-7015 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM... Clauses 252.247-7015 Requirements. As prescribed in 216.506(d), substitute the following paragraph (f) for...

48 CFR 252.247-7015 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Requirements. 252.247-7015 Section 252.247-7015 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM... Clauses 252.247-7015 Requirements. As prescribed in 216.506(d), substitute the following paragraph (f) for...

48 CFR 252.247-7015 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Requirements. 252.247-7015 Section 252.247-7015 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM... Clauses 252.247-7015 Requirements. As prescribed in 216.506(d), substitute the following paragraph (f) for...

48 CFR 644.302 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirements. 644.302 Section 644.302 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractor's Purchasing System Reviews 644.302 Requirements. (a) The...

48 CFR 2944.302 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Requirements. 2944.302 Section 2944.302 Federal Acquisition Regulations System DEPARTMENT OF LABOR CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractors' Purchasing Systems Reviews 2944.302 Requirements. The...

48 CFR 644.302 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirements. 644.302 Section 644.302 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractor's Purchasing System Reviews 644.302 Requirements. (a) The...

48 CFR 2944.302 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Requirements. 2944.302 Section 2944.302 Federal Acquisition Regulations System DEPARTMENT OF LABOR CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractors' Purchasing Systems Reviews 2944.302 Requirements. The...

48 CFR 644.302 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirements. 644.302 Section 644.302 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractor's Purchasing System Reviews 644.302 Requirements. (a) The...

48 CFR 644.302 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirements. 644.302 Section 644.302 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractor's Purchasing System Reviews 644.302 Requirements. (a) The...

48 CFR 1344.302 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirements. 1344.302 Section 1344.302 Federal Acquisition Regulations System DEPARTMENT OF COMMERCE CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractors' Purchasing Systems Reviews § 1344.302 Requirements. The...

48 CFR 2944.302 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Requirements. 2944.302 Section 2944.302 Federal Acquisition Regulations System DEPARTMENT OF LABOR CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractors' Purchasing Systems Reviews 2944.302 Requirements. The...

48 CFR 644.302 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirements. 644.302 Section 644.302 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACT MANAGEMENT SUBCONTRACTING POLICIES AND PROCEDURES Contractor's Purchasing System Reviews 644.302 Requirements. (a) The...

48 CFR 2806.303-1 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Requirements. 2806.303-1 Section 2806.303-1 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 2806.303-1 Requirements...

48 CFR 706.303-1 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirements. 706.303-1 Section 706.303-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 706.303-1 Requirements. (a)-(c...

48 CFR 606.303-1 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirements. 606.303-1 Section 606.303-1 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 606.303-1 Requirements...

48 CFR 606.303-1 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirements. 606.303-1 Section 606.303-1 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 606.303-1 Requirements...

48 CFR 706.303-1 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirements. 706.303-1 Section 706.303-1 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 706.303-1 Requirements. (a)-(c...

48 CFR 2806.303-1 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Requirements. 2806.303-1 Section 2806.303-1 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 2806.303-1 Requirements...

48 CFR 606.303-1 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirements. 606.303-1 Section 606.303-1 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 606.303-1 Requirements...

48 CFR 2806.303-1 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Requirements. 2806.303-1 Section 2806.303-1 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE Competition and Acquisition Planning COMPETITION REQUIREMENTS Other Than Full and Open Competition 2806.303-1 Requirements...

48 CFR 606.303-1 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirements. 606.303-1 Section 606.303-1 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 606.303-1 Requirements...

48 CFR 606.303-1 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirements. 606.303-1 Section 606.303-1 Federal Acquisition Regulations System DEPARTMENT OF STATE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 606.303-1 Requirements...

48 CFR 2806.303-1 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Requirements. 2806.303-1 Section 2806.303-1 Federal Acquisition Regulations System DEPARTMENT OF JUSTICE COMPETITION AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 2806.303-1 Requirements...

49 CFR 582.4 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false Requirements. 582.4 Section 582.4 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) INSURANCE COST INFORMATION REGULATION § 582.4 Requirements. (a) Each...

49 CFR 582.4 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false Requirements. 582.4 Section 582.4 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) INSURANCE COST INFORMATION REGULATION § 582.4 Requirements. (a) Each...

49 CFR 582.4 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false Requirements. 582.4 Section 582.4 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) INSURANCE COST INFORMATION REGULATION § 582.4 Requirements. (a) Each...

49 CFR 582.4 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false Requirements. 582.4 Section 582.4 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) INSURANCE COST INFORMATION REGULATION § 582.4 Requirements. (a) Each...

49 CFR 582.4 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false Requirements. 582.4 Section 582.4 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION (CONTINUED) INSURANCE COST INFORMATION REGULATION § 582.4 Requirements. (a) Each...

48 CFR 29.304 - Matters requiring special consideration.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Matters requiring special consideration. 29.304 Section 29.304 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS TAXES State and Local Taxes 29.304 Matters requiring special...

48 CFR 29.304 - Matters requiring special consideration.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Matters requiring special consideration. 29.304 Section 29.304 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS TAXES State and Local Taxes 29.304 Matters requiring special...

48 CFR 29.304 - Matters requiring special consideration.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Matters requiring special consideration. 29.304 Section 29.304 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS TAXES State and Local Taxes 29.304 Matters requiring special...

48 CFR 29.304 - Matters requiring special consideration.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Matters requiring special consideration. 29.304 Section 29.304 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS TAXES State and Local Taxes 29.304 Matters requiring special...

48 CFR 29.304 - Matters requiring special consideration.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Matters requiring special consideration. 29.304 Section 29.304 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS TAXES State and Local Taxes 29.304 Matters requiring special...

48 CFR 970.2670-1 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirements. 970.2670-1... REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Other Socioeconomic Programs 970.2670-1 Requirements. The goal requirements of section 3021 of the Energy Policy Act of 1992, and the attendant reporting...

48 CFR 970.2670-1 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirements. 970.2670-1... REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Other Socioeconomic Programs 970.2670-1 Requirements. The goal requirements of section 3021 of the Energy Policy Act of 1992, and the attendant reporting...

Transcription regulation of the Saccharomyces cerevisiae PIS1 gene by inositol and the pleiotropic regulator, Ume6p.

PubMed

Jani, Niketa M; Lopes, John M

2008-12-01

In Saccharomyces cerevisiae, transcription of most of the phospholipid biosynthetic genes (e.g. INO1, CHO1, CHO2 and OPI3) is repressed by growth in the presence of inositol and choline and derepressed in their absence. This regulation requires the Ino2p and Ino4p activators and the Opi1p repressor. The PIS1 structural gene is required for the synthesis of the essential lipid phosphatidylinositol. Previous reports show that PIS1 expression is uncoupled from inositol/choline regulation, but is regulated by carbon source, hypoxia and zinc. However, in this study we found that the expression of PIS1 is induced twofold by inositol. This regulation did not require Ino2p and Ino4p, although Ino4p was required for full expression. Ino4p is a basic helix-loop-helix protein that requires a binding partner. Curiously, none of the other basic helix-loop-helix proteins affected PIS1 expression. Inositol induction did require another general regulator of phospholipid biosynthesis, Ume6p. Ume6p was found to be a positive regulator of PIS1 gene expression. Ume6p, and several associated factors, were required for inositol-mediated induction and chromatin immunoprecipitation analysis showed that Ume6p directly regulates PIS1 expression. Thus, we demonstrate novel regulation of the PIS1 gene by Ume6p.

Distinct Akt phosphorylation states are required for insulin regulated Glut4 and Glut1-mediated glucose uptake.

PubMed

Beg, Muheeb; Abdullah, Nazish; Thowfeik, Fathima Shazna; Altorki, Nasser K; McGraw, Timothy E

2017-06-07

Insulin, downstream of Akt activation, promotes glucose uptake into fat and muscle cells to lower postprandial blood glucose, an enforced change in cellular metabolism to maintain glucose homeostasis. This effect is mediated by the Glut4 glucose transporter. Growth factors also enhance glucose uptake to fuel an anabolic metabolism required for tissue growth and repair. This activity is predominantly mediated by the Glut1. Akt is activated by phosphorylation of its kinase and hydrophobic motif (HM) domains. We show that insulin-stimulated Glut4-mediated glucose uptake requires PDPK1 phosphorylation of the kinase domain but not mTORC2 phosphorylation of the HM domain. Nonetheless, an intact HM domain is required for Glut4-mediated glucose uptake. Whereas, Glut1-mediated glucose uptake also requires mTORC2 phosphorylation of the HM domain, demonstrating both phosphorylation-dependent and independent roles of the HM domain in regulating glucose uptake. Thus, mTORC2 links Akt to the distinct physiologic programs related to Glut4 and Glut1-mediated glucose uptake.

76 FR 53886 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Special...

Federal Register 2010, 2011, 2012, 2013, 2014

2011-08-30

...; Defense Federal Acquisition Regulation Supplement; Special Contracting Methods AGENCY: Defense Acquisition...-0214, using any of the following methods: [cir] Regulations.gov : http://www.regulations.gov . Follow... Supplement (DFARS) Part 217, Special Contracting Methods, and related provisions and clauses at DFARS 252.217...

48 CFR 3439.702 - Department security requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... requirements. 3439.702 Section 3439.702 Federal Acquisition Regulations System DEPARTMENT OF EDUCATION ACQUISITION REGULATION SPECIAL CATEGORIES OF CONTRACTING ACQUISITION OF INFORMATION TECHNOLOGY Department Requirements for Acquisition of Information Technology 3439.702 Department security requirements. The...

Interdomain allosteric regulation of Polo kinase by Aurora B and Map205 is required for cytokinesis

PubMed Central

Kachaner, David; Pinson, Xavier; El Kadhi, Khaled Ben; Normandin, Karine; Talje, Lama; Lavoie, Hugo; Lépine, Guillaume; Carréno, Sébastien; Kwok, Benjamin H.; Hickson, Gilles R.

2014-01-01

Drosophila melanogaster Polo and its human orthologue Polo-like kinase 1 fulfill essential roles during cell division. Members of the Polo-like kinase (Plk) family contain an N-terminal kinase domain (KD) and a C-terminal Polo-Box domain (PBD), which mediates protein interactions. How Plks are regulated in cytokinesis is poorly understood. Here we show that phosphorylation of Polo by Aurora B is required for cytokinesis. This phosphorylation in the activation loop of the KD promotes the dissociation of Polo from the PBD-bound microtubule-associated protein Map205, which acts as an allosteric inhibitor of Polo kinase activity. This mechanism allows the release of active Polo from microtubules of the central spindle and its recruitment to the site of cytokinesis. Failure in Polo phosphorylation results in both early and late cytokinesis defects. Importantly, the antagonistic regulation of Polo by Aurora B and Map205 in cytokinesis reveals that interdomain allosteric mechanisms can play important roles in controlling the cellular functions of Plks. PMID:25332165

48 CFR 752.204-2 - Security requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Security requirements. 752.204-2 Section 752.204-2 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT... Security requirements. Pursuant to the Uniform State/USAID/USIA Regulations (Volume 12, Foreign Affairs...

48 CFR 752.204-2 - Security requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 5 2013-10-01 2013-10-01 false Security requirements. 752.204-2 Section 752.204-2 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT... Security requirements. Pursuant to the Uniform State/USAID/USIA Regulations (Volume 12, Foreign Affairs...

48 CFR 752.204-2 - Security requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Security requirements. 752.204-2 Section 752.204-2 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT... Security requirements. Pursuant to the Uniform State/USAID/USIA Regulations (Volume 12, Foreign Affairs...

48 CFR 752.204-2 - Security requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false Security requirements. 752.204-2 Section 752.204-2 Federal Acquisition Regulations System AGENCY FOR INTERNATIONAL DEVELOPMENT... Security requirements. Pursuant to the Uniform State/USAID/USIA Regulations (Volume 12, Foreign Affairs...

[Legal framework of data protection : current requirements in Germany and requirements in planned European Union regulations].

PubMed

Schütze, B

2013-05-01

The federal system in Germany necessitates that in addition to federal laws, country and church-specific legislations must also be considered during the evaluation of relevant legal stipulations concerning data protection. Furthermore, there are also special legal regulations for hospitals in almost every federal state which are governed by the principle of subsidiarity: special legal regulations are to be preferentially used, so that findings from one federal state are difficult to transfer to another federal state.Patient data may only be used and processed without legal regulations with informed consent of the patient. The use of patient data for purposes of quality assurance, research and further education of students and doctors is possible under the present laws according to a positive weighting of interests. Patient data can also be exchanged via online services for the purposes of patient care; however, informed consent of the patient for medical online services is almost always unavoidable.

48 CFR 432.703 - Contract funding requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Contract funding requirements. 432.703 Section 432.703 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.703 Contract funding requirements. ...

48 CFR 2932.703 - Contract funding requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Contract funding requirements. 2932.703 Section 2932.703 Federal Acquisition Regulations System DEPARTMENT OF LABOR GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 2932.703 Contract funding requirements. (a...

48 CFR 632.703 - Contract funding requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Contract funding requirements. 632.703 Section 632.703 Federal Acquisition Regulations System DEPARTMENT OF STATE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 632.703 Contract funding requirements. ...

48 CFR 632.703 - Contract funding requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Contract funding requirements. 632.703 Section 632.703 Federal Acquisition Regulations System DEPARTMENT OF STATE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 632.703 Contract funding requirements. ...

48 CFR 432.703 - Contract funding requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Contract funding requirements. 432.703 Section 432.703 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 432.703 Contract funding requirements. ...

48 CFR 2932.703 - Contract funding requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Contract funding requirements. 2932.703 Section 2932.703 Federal Acquisition Regulations System DEPARTMENT OF LABOR GENERAL CONTRACTING REQUIREMENTS CONTRACT FINANCING Contract Funding 2932.703 Contract funding requirements. (a...

29 CFR 1926.1091 - Recordkeeping requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... Labor Regulations Relating to Labor (Continued) OCCUPATIONAL SAFETY AND HEALTH ADMINISTRATION, DEPARTMENT OF LABOR (CONTINUED) SAFETY AND HEALTH REGULATIONS FOR CONSTRUCTION Diving Recordkeeping § 1926.1091 Recordkeeping requirements. Note: The requirements applicable to construction work under this...

Light-Regulated Thyroid Hormone Signaling Is Required for Rod Photoreceptor Development in the Mouse Retina.

PubMed

Sawant, Onkar; Horton, Amanda M; Shukla, Meenal; Rayborn, Mary E; Peachey, Neal S; Hollyfield, Joe G; Rao, Sujata

2015-12-01

Ambient light is both a stimulus for visual function and a regulator of photoreceptor physiology. However, it is not known if light can regulate any aspect of photoreceptor development. The purpose of this study was to investigate whether ambient light is required for the development of mouse rod photoreceptors. Newborn mouse pups (C57BL/6) were reared in either cyclic light (LD) or constant dark (DD). Pups were collected at postnatal day (P)5, P10, P17, or P24. We performed retinal morphometric and cell death analysis at P5, P10, and P17. Rhodopsin expression was assessed using immunofluorescence, Western blot, and quantitative RT-PCR analysis. Electroretinograms were performed at P17 and P24. Radioimmunoassay and ELISA were used to follow changes in thyroid hormone levels in the serum and vitreous. In the DD pups, the outer nuclear layer was significantly thinner at P10 and there were higher numbers of apoptotic cells at P5 compared to the LD pups. Rhodopsin expression was lower at P10 and P17 in DD pups. Electroretinogram a-waves were reduced in amplitude at P17 in the DD pups. The DD animals had lower levels of circulating thyroid hormones at P10. Light-mediated changes in thyroid hormones occur as early as P5, as we detected lower levels of total triiodothyronine in the vitreous from the DD animals. Drug-induced developmental hypothyroidism resulted in lower rhodopsin expression at P10. Our data demonstrate that light exposure during postnatal development is required for rod photoreceptor development and that this effect could be mediated by thyroid hormone signaling.

75 FR 54041 - Insurer Reporting Requirements; List of Insurers Required To File Reports

Federal Register 2010, 2011, 2012, 2013, 2014

2010-09-03

... and Franchisees) Subject to the Reporting Requirements of Part 544 Cendant Car Rental Dollar Thrifty... [Docket No. NHTSA-2010-0017] RIN 2127-AK69 Insurer Reporting Requirements; List of Insurers Required To... Reporting Requirements. The regulations specify the requirements for annual insurer reports and lists in...

A Distal ABA Responsive Element in AtNCED3 Promoter Is Required for Positive Feedback Regulation of ABA Biosynthesis in Arabidopsis

PubMed Central

Yang, Yan-Zhuo; Tan, Bao-Cai

2014-01-01

The plant hormone abscisic acid (ABA) plays a crucial role in plant development and responses to abiotic stresses. Recent studies indicate that a positive feedback regulation by ABA exists in ABA biosynthesis in plants under dehydration stress. To understand the molecular basis of this regulation, we analyzed the cis-elements of the AtNCED3 promoter in Arabidopsis. AtNCED3 encodes the first committed and highly regulated dioxygenase in the ABA biosynthetic pathway. Through delineated and mutagenesis analyses in stable-transformed Arabidopsis, we revealed that a distal ABA responsive element (ABRE: GGCACGTG, -2372 to -2364 bp) is required for ABA-induced AtNCED3 expression. By analyzing the AtNCED3 expression in ABRE binding protein ABF3 over-expression transgenic plants and knock-out mutants, we provide evidence that the ABA feedback regulation of AtNCED3 expression is not mediated by ABF3. PMID:24475264

A distal ABA responsive element in AtNCED3 promoter is required for positive feedback regulation of ABA biosynthesis in Arabidopsis.

PubMed

Yang, Yan-Zhuo; Tan, Bao-Cai

2014-01-01

The plant hormone abscisic acid (ABA) plays a crucial role in plant development and responses to abiotic stresses. Recent studies indicate that a positive feedback regulation by ABA exists in ABA biosynthesis in plants under dehydration stress. To understand the molecular basis of this regulation, we analyzed the cis-elements of the AtNCED3 promoter in Arabidopsis. AtNCED3 encodes the first committed and highly regulated dioxygenase in the ABA biosynthetic pathway. Through delineated and mutagenesis analyses in stable-transformed Arabidopsis, we revealed that a distal ABA responsive element (ABRE: GGCACGTG, -2372 to -2364 bp) is required for ABA-induced AtNCED3 expression. By analyzing the AtNCED3 expression in ABRE binding protein ABF3 over-expression transgenic plants and knock-out mutants, we provide evidence that the ABA feedback regulation of AtNCED3 expression is not mediated by ABF3.

A two-component response regulator, gltR, is required for glucose transport activity in Pseudomonas aeruginosa PAO1.

PubMed Central

Sage, A E; Proctor, W D; Phibbs, P V

1996-01-01

A 729-bp open reading frame (gltR) was identified in Pseudomonas aeruginosa PAO1 that encodes a product homologous to the two-component response regulator family of proteins. Disruption of gltR caused loss of glucose transport activity. Restoration of gltR resulted in wild-type levels of glucose transport. These findings indicate that gltR is required for expression of the glucose transport system in P. aeruginosa. PMID:8830708

5 CFR 340.201 - Regulatory requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 340.201 Administrative Personnel OFFICE OF PERSONNEL MANAGEMENT CIVIL SERVICE REGULATIONS OTHER THAN FULL-TIME CAREER EMPLOYMENT (PART-TIME, SEASONAL, ON-CALL, AND INTERMITTENT) Regulatory Requirements-Part-Time Employment § 340.201 Regulatory requirements. This subpart contains the regulations of the...

48 CFR 23.1004 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Requirements. 23.1004 Section 23.1004 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND...

48 CFR 23.1004 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Requirements. 23.1004 Section 23.1004 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND...

48 CFR 23.601 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 1 2012-10-01 2012-10-01 false Requirements. 23.601 Section 23.601 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND...

48 CFR 242.7302 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Requirements. 242.7302 Section 242.7302 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contractor Insurance...

48 CFR 1426.7103-2 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirements. 1426.7103-2 Section 1426.7103-2 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR SOCIOECONOMIC PROGRAMS OTHER SOCIOECONOMIC PROGRAMS Minority Business Reports 1426.7103-2 Requirements. The contracting...

Precision Adjustable Liquid Regulator (ALR)

NASA Astrophysics Data System (ADS)

Meinhold, R.; Parker, M.

2004-10-01

A passive mechanical regulator has been developed for the control of fuel or oxidizer flow to a 450N class bipropellant engine for use on commercial and interplanetary spacecraft. There are several potential benefits to the propulsion system, depending on mission requirements and spacecraft design. This system design enables more precise control of main engine mixture ratio and inlet pressure, and simplifies the pressurization system by transferring the function of main engine flow rate control from the pressurization/propellant tank assemblies, to a single component, the ALR. This design can also reduce the thermal control requirements on the propellant tanks, avoid costly Qualification testing of biprop engines for missions with more stringent requirements, and reduce the overall propulsion system mass and power usage. In order to realize these benefits, the ALR must meet stringent design requirements. The main advantage of this regulator over other units available in the market is that it can regulate about its nominal set point to within +/-0.85%, and change its regulation set point in flight +/-4% about that nominal point. The set point change is handled actively via a stepper motor driven actuator, which converts rotary into linear motion to affect the spring preload acting on the regulator. Once adjusted to a particular set point, the actuator remains in its final position unpowered, and the regulator passively maintains outlet pressure. The very precise outlet regulation pressure is possible due to new technology developed by Moog, Inc. which reduces typical regulator mechanical hysteresis to near zero. The ALR requirements specified an outlet pressure set point range from 225 to 255 psi, and equivalent water flow rates required were in the 0.17 lb/sec range. The regulation output pressure is maintained at +/-2 psi about the set point from a P (delta or differential pressure) of 20 to over 100 psid. Maximum upstream system pressure was specified at 320 psi

12 CFR 722.3 - Appraisals required; transactions requiring a State certified or licensed appraiser.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 12 Banks and Banking 6 2010-01-01 2010-01-01 false Appraisals required; transactions requiring a... ADMINISTRATION REGULATIONS AFFECTING CREDIT UNIONS APPRAISALS § 722.3 Appraisals required; transactions requiring... certified or licensed appraiser is required for all real estate-related financial transactions except those...

EU Regulation of Nanobiocides: Challenges in Implementing the Biocidal Product Regulation (BPR)

PubMed Central

Brinch, Anna; Hansen, Steffen Foss; Hartmann, Nanna B.; Baun, Anders

2016-01-01

The Biocidal Products Regulation (BPR) contains several provisions for nanomaterials (NMs) and is the first regulation in the European Union to require specific testing and risk assessment for the NM form of a biocidal substance as a part of the information requirements. Ecotoxicological data are one of the pillars of the information requirements in the BPR, but there are currently no standard test guidelines for the ecotoxicity testing of NMs. The overall objective of this work was to investigate the implications of the introduction of nano-specific testing requirements in the BPR and to explore how these might be fulfilled in the case of copper oxide nanoparticles. While there is information and data available in the open literature that could be used to fulfill the BPR information requirements, most of the studies do not take the Organisation for Economic Co-operation and Development’s nanospecific test guidelines into consideration. This makes it difficult for companies as well as regulators to fulfill the BPR information requirements for nanomaterials. In order to enable a nanospecific risk assessment, best practices need to be developed regarding stock suspension preparation and characterization, exposure suspensions preparation, and for conducting ecotoxicological test. PMID:28344290

EU Regulation of Nanobiocides: Challenges in Implementing the Biocidal Product Regulation (BPR).

PubMed

Brinch, Anna; Hansen, Steffen Foss; Hartmann, Nanna B; Baun, Anders

2016-02-16

The Biocidal Products Regulation (BPR) contains several provisions for nanomaterials (NMs) and is the first regulation in the European Union to require specific testing and risk assessment for the NM form of a biocidal substance as a part of the information requirements. Ecotoxicological data are one of the pillars of the information requirements in the BPR, but there are currently no standard test guidelines for the ecotoxicity testing of NMs. The overall objective of this work was to investigate the implications of the introduction of nano-specific testing requirements in the BPR and to explore how these might be fulfilled in the case of copper oxide nanoparticles. While there is information and data available in the open literature that could be used to fulfill the BPR information requirements, most of the studies do not take the Organisation for Economic Co-operation and Development's nanospecific test guidelines into consideration. This makes it difficult for companies as well as regulators to fulfill the BPR information requirements for nanomaterials. In order to enable a nanospecific risk assessment, best practices need to be developed regarding stock suspension preparation and characterization, exposure suspensions preparation, and for conducting ecotoxicological test.

48 CFR 408.1103 - Contract requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Contract requirements. 408.1103 Section 408.1103 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE COMPETITION AND ACQUISITION PLANNING REQUIRED SOURCES OF SUPPLIES AND SERVICES Leasing of Motor Vehicles 408.1103 Contract...

48 CFR 1426.7102-2 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 5 2014-10-01 2014-10-01 false Requirements. 1426.7102-2 Section 1426.7102-2 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR SOCIOECONOMIC PROGRAMS OTHER SOCIOECONOMIC PROGRAMS Minority Business Reports 1426.7102-2 Requirements. (a) MBDA-91 Plan...

48 CFR 1426.7102-2 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Requirements. 1426.7102-2 Section 1426.7102-2 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR SOCIOECONOMIC PROGRAMS OTHER SOCIOECONOMIC PROGRAMS Minority Business Reports 1426.7102-2 Requirements. (a) MBDA-91 Plan...

7 CFR 3015.161 - Additional requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Additional requirements. 3015.161 Section 3015.161 Agriculture Regulations of the Department of Agriculture (Continued) OFFICE OF THE CHIEF FINANCIAL OFFICER, DEPARTMENT OF AGRICULTURE UNIFORM FEDERAL ASSISTANCE REGULATIONS Property § 3015.161 Additional requirements...

7 CFR 1774.7 - Environmental requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 12 2011-01-01 2011-01-01 false Environmental requirements. 1774.7 Section 1774.7 Agriculture Regulations of the Department of Agriculture (Continued) RURAL UTILITIES SERVICE, DEPARTMENT OF...) General Provisions § 1774.7 Environmental requirements. The policies and regulations contained in 7 CFR...

48 CFR 323.7002 - Actions required.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Actions required. 323.7002 Section 323.7002 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES SOCIOECONOMIC PROGRAMS... WORKPLACE Safety and Health 323.7002 Actions required. (a) Contracting activities. The Contracting Officer...

Notification: Hotline Complaint – Region 7 Compliance with Bid, Payment, and Performance Bond Requirements Outlined in Federal Acquisition Regulation (FAR) Part 28

EPA Pesticide Factsheets

June 1, 2012. In response to an anonymous hotline complaint, the OIG plans to conduct a review of Region 7’s compliance with bid, payment, and performance bond requirements outlined in Federal Acquisition Regulation (FAR) Part 28.

Preschool Regulations.

ERIC Educational Resources Information Center

Nebraska State Dept. of Health and Human Services, Lincoln.

Published by the Department of Health and Human Services, as required by Nebraska law, this guide details regulations for the physical well-being, safety, and protection of children and defines the minimum levels of acceptable services to be provided in Nebraska preschools. The first section of the guide lists specific preschool regulations,…

16 CFR 1512.12 - Requirements for wheel hubs.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 16 Commercial Practices 2 2014-01-01 2014-01-01 false Requirements for wheel hubs. 1512.12 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.12 Requirements for wheel hubs. All bicycles (other than sidewalk bicycles) shall meet the following requirements: (a) Locking devices. Wheels shall be...

16 CFR 1512.12 - Requirements for wheel hubs.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 16 Commercial Practices 2 2012-01-01 2012-01-01 false Requirements for wheel hubs. 1512.12 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.12 Requirements for wheel hubs. All bicycles (other than sidewalk bicycles) shall meet the following requirements: (a) Locking devices. Wheels shall be...

The European Union's REACH regulation: a review of its history and requirements.

PubMed

Williams, E Spencer; Panko, Julie; Paustenbach, Dennis J

2009-01-01

In 2006, the European Union (EU) promulgated a monumental regulatory initiative for the Registration, Evaluation, Authorization, and Restriction of Chemicals (REACH). To date, several thousand pages of text have been needed to describe the expectations of this regulation. There were numerous reasons for the promulgation of REACH, but, by and large, it is an extension of the global desire to produce fewer industrial chemicals, to understand the possible human and ecological hazards of those that are produced, and to insure that any major threat is anticipated, as well as prevented. Most industry-related groups consider it the most wide-ranging and costly regulatory initiatives related to health risk assessment ever to be promulgated. This review presents a description of REACH that should inform scientists, managers, and others about its objectives and the means to satisfy them. Registration is required for all chemicals manufactured or imported into the EU, unless specifically exempted. Registration is expected to be a collaborative process among companies, which will generate a dossier containing data on physicochemical characteristics, as well as toxicological and ecotoxicological properties. Though the magnitude of the gaps in the data required for registration is uncertain at this point, it is clear that basic toxicology testing will have to be conducted for many chemical substances that have not undergone formal review up to this point. For many chemicals, an examination of hazards and risks arising from the use of these substances will also be required in the form of a chemical safety report (CSR). Beginning with the dual processes of dossier and substance evaluation, the European Chemicals Agency (ECHA), the Member States of the EU, and the European Commission will identify chemicals that may pose unacceptable hazards to human health and/or the environment, and will curtail or restrict their usage. The implementation of REACH will expand and deepen the

16 CFR 1512.10 - Requirements for tires.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for tires. 1512.10 Section 1512... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.10 Requirements for tires. The manufacturer's recommended inflation pressure shall be molded into or onto the sidewall of the tire in lettering no less than...

16 CFR 304.2 - General requirement.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false General requirement. 304.2 Section 304.2 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE HOBBY PROTECTION ACT § 304.2 General requirement. Imitation political or numismatic...

16 CFR 300.2 - General requirement.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 1 2011-01-01 2011-01-01 false General requirement. 300.2 Section 300.2 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.2 General requirement. Each and every...

40 CFR 141.500 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 141.500 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and Disinfection-Systems Serving... subpart constitute national primary drinking water regulations. These regulations establish requirements...

40 CFR 141.170 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 141.170 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Enhanced Filtration and Disinfection-Systems Serving... constitute national primary drinking water regulations. These regulations establish requirements for...

Myosin IIb-dependent Regulation of Actin Dynamics Is Required for N-Methyl-D-aspartate Receptor Trafficking during Synaptic Plasticity.

PubMed

Bu, Yunfei; Wang, Ning; Wang, Shaoli; Sheng, Tao; Tian, Tian; Chen, Linlin; Pan, Weiwei; Zhu, Minsheng; Luo, Jianhong; Lu, Wei

2015-10-16

N-Methyl-d-aspartate receptor (NMDAR) synaptic incorporation changes the number of NMDARs at synapses and is thus critical to various NMDAR-dependent brain functions. To date, the molecules involved in NMDAR trafficking and the underlying mechanisms are poorly understood. Here, we report that myosin IIb is an essential molecule in NMDAR synaptic incorporation during PKC- or θ burst stimulation-induced synaptic plasticity. Moreover, we demonstrate that myosin light chain kinase (MLCK)-dependent actin reorganization contributes to NMDAR trafficking. The findings from additional mutual occlusion experiments demonstrate that PKC and MLCK share a common signaling pathway in NMDAR-mediated synaptic regulation. Because myosin IIb is the primary substrate of MLCK and can regulate actin dynamics during synaptic plasticity, we propose that the MLCK- and myosin IIb-dependent regulation of actin dynamics is required for NMDAR trafficking during synaptic plasticity. This study provides important insights into a mechanical framework for understanding NMDAR trafficking associated with synaptic plasticity. © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

48 CFR 3023.1004 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Requirements. 3023.1004...-Know Laws and Pollution Requirements 3023.1004 Requirements. DHS Directive 023-02 Environmental... contain appropriate sustainable practices requirements, provisions and clauses. Contractors shall support...

48 CFR 3023.1004 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Requirements. 3023.1004...-Know Laws and Pollution Requirements 3023.1004 Requirements. DHS Directive 023-02 Environmental... contain appropriate sustainable practices requirements, provisions and clauses. Contractors shall support...

48 CFR 3023.1004 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Requirements. 3023.1004...-Know Laws and Pollution Requirements 3023.1004 Requirements. DHS Directive 023-02 Environmental... contain appropriate sustainable practices requirements, provisions and clauses. Contractors shall support...

76 FR 81408 - Contractor Legal Management Requirements; Acquisition Regulations

Federal Register 2010, 2011, 2012, 2013, 2014

2011-12-28

... DEPARTMENT OF ENERGY 10 CFR Part 719 48 CFR Parts 931, 952 and 970 RIN 1990-AA37 Contractor Legal... Energy (DOE or Department) is proposing to revise existing regulations covering contractor legal... costs by certain contractors whose contracts exceed $100,000,000 as well as legal counsel retained...

78 FR 25795 - Contractor Legal Management Requirements; Acquisition Regulations

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

... DEPARTMENT OF ENERGY 10 CFR Part 719 48 CFR Parts 931, 952, and 970 RIN 1990-AA37 Contractor Legal.... ACTION: Final rule. SUMMARY: The Department of Energy revises existing regulations covering contractor... costs by certain contractors whose contracts exceed $100,000,000 as well as legal counsel retained...

48 CFR 2906.501 - Requirement.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 7 2012-10-01 2012-10-01 false Requirement. 2906.501... COMPETITION REQUIREMENTS Competition Advocate 2906.501 Requirement. The Assistant Secretary for Administration... be predicated on an understanding of the competition requirements in the FAR, and particularly small...

48 CFR 2906.501 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 7 2010-10-01 2010-10-01 false Requirement. 2906.501... COMPETITION REQUIREMENTS Competition Advocate 2906.501 Requirement. The Assistant Secretary for Administration... be predicated on an understanding of the competition requirements in the FAR, and particularly small...

48 CFR 2906.501 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 7 2014-10-01 2014-10-01 false Requirement. 2906.501... COMPETITION REQUIREMENTS Competition Advocate 2906.501 Requirement. The Assistant Secretary for Administration... be predicated on an understanding of the competition requirements in the FAR, and particularly small...

48 CFR 2906.501 - Requirement.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 7 2013-10-01 2012-10-01 true Requirement. 2906.501... COMPETITION REQUIREMENTS Competition Advocate 2906.501 Requirement. The Assistant Secretary for Administration... be predicated on an understanding of the competition requirements in the FAR, and particularly small...

75 FR 33271 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement; Foreign...

Federal Register 2010, 2011, 2012, 2013, 2014

2010-06-11

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [OMB Control Number 0704-0229... AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and request... Certain Contracts with Foreign Entities for the Conduct of Ballistic Missile Defense Research, Development...

40 CFR 141.620 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 141.620 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) WATER PROGRAMS (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Stage 2 Disinfection Byproducts Requirements § 141... primary drinking water regulations. The regulations in this subpart establish monitoring and other...

Beclin 1 Is Required for Neuron Viability and Regulates Endosome Pathways via the UVRAG-VPS34 Complex

PubMed Central

Wold, Mitchell S.; Gong, Shiaoching; Phillips, Greg R.; Dou, Zhixun; Zhao, Yanxiang; Heintz, Nathaniel; Zong, Wei-Xing; Yue, Zhenyu

2014-01-01

Deficiency of autophagy protein beclin 1 is implicated in tumorigenesis and neurodegenerative diseases, but the molecular mechanism remains elusive. Previous studies showed that Beclin 1 coordinates the assembly of multiple VPS34 complexes whose distinct phosphatidylinositol 3-kinase III (PI3K-III) lipid kinase activities regulate autophagy at different steps. Recent evidence suggests a function of beclin 1 in regulating multiple VPS34-mediated trafficking pathways beyond autophagy; however, the precise role of beclin 1 in autophagy-independent cellular functions remains poorly understood. Herein we report that beclin 1 regulates endocytosis, in addition to autophagy, and is required for neuron viability in vivo. We find that neuronal beclin 1 associates with endosomes and regulates EEA1/early endosome localization and late endosome formation. Beclin 1 maintains proper cellular phosphatidylinositol 3-phosphate (PI(3)P) distribution and total levels, and loss of beclin 1 causes a disruption of active Rab5 GTPase-associated endosome formation and impairment of endosome maturation, likely due to a failure of Rab5 to recruit VPS34. Furthermore, we find that Beclin 1 deficiency causes complete loss of the UVRAG-VPS34 complex and associated lipid kinase activity. Interestingly, beclin 1 deficiency impairs p40phox-linked endosome formation, which is rescued by overexpressed UVRAG or beclin 1, but not by a coiled-coil domain-truncated beclin 1 (a UVRAG-binding mutant), Atg14L or RUBICON. Thus, our study reveals the essential role for beclin 1 in neuron survival involving multiple membrane trafficking pathways including endocytosis and autophagy, and suggests that the UVRAG-beclin 1 interaction underlies beclin 1's function in endocytosis. PMID:25275521

7 CFR 1948.63 - Historic preservation requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 13 2010-01-01 2009-01-01 true Historic preservation requirements. 1948.63 Section 1948.63 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE... Development Assistance Program § 1948.63 Historic preservation requirements. The policies and regulations...

31 CFR 900.7 - Required administrative proceedings.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 31 Money and Finance:Treasury 3 2013-07-01 2013-07-01 false Required administrative proceedings. 900.7 Section 900.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... administrative proceedings required by contract or other laws or regulations. ...

31 CFR 900.7 - Required administrative proceedings.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 31 Money and Finance:Treasury 3 2014-07-01 2014-07-01 false Required administrative proceedings. 900.7 Section 900.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... administrative proceedings required by contract or other laws or regulations. ...

31 CFR 900.7 - Required administrative proceedings.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 31 Money and Finance:Treasury 3 2012-07-01 2012-07-01 false Required administrative proceedings. 900.7 Section 900.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... administrative proceedings required by contract or other laws or regulations. ...

31 CFR 900.7 - Required administrative proceedings.

Code of Federal Regulations, 2011 CFR

2011-07-01

... 31 Money and Finance:Treasury 3 2011-07-01 2011-07-01 false Required administrative proceedings. 900.7 Section 900.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... administrative proceedings required by contract or other laws or regulations. ...

31 CFR 900.7 - Required administrative proceedings.

Code of Federal Regulations, 2010 CFR

2010-07-01

... 31 Money and Finance: Treasury 3 2010-07-01 2010-07-01 false Required administrative proceedings. 900.7 Section 900.7 Money and Finance: Treasury Regulations Relating to Money and Finance (Continued... administrative proceedings required by contract or other laws or regulations. ...

77 FR 58817 - Information Collection Requirement; Defense Federal Acquisition Regulation Supplement (DFARS...

Federal Register 2010, 2011, 2012, 2013, 2014

2012-09-24

... DEPARTMENT OF DEFENSE Defense Acquisition Regulations System [OMB Control Number 0704-0321... Financing AGENCY: Defense Acquisition Regulations System, Department of Defense (DoD). ACTION: Notice and... . Paper copies are available from Mr. Mark Gomersall, OUSD(AT&L) DPAP/DARS, Room 3B855, 3060 Defense...

48 CFR 242.1106 - Reporting requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Reporting requirements. 242.1106 Section 242.1106 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Production...

48 CFR 242.1104 - Surveillance requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Surveillance requirements. 242.1104 Section 242.1104 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Production...

Proteolytic cleavage and PKA phosphorylation of α1C subunit are not required for adrenergic regulation of CaV1.2 in the heart.

PubMed

Katchman, Alexander; Yang, Lin; Zakharov, Sergey I; Kushner, Jared; Abrams, Jeffrey; Chen, Bi-Xing; Liu, Guoxia; Pitt, Geoffrey S; Colecraft, Henry M; Marx, Steven O

2017-08-22

Calcium influx through the voltage-dependent L-type calcium channel (Ca V 1.2) rapidly increases in the heart during "fight or flight" through activation of the β-adrenergic and protein kinase A (PKA) signaling pathway. The precise molecular mechanisms of β-adrenergic activation of cardiac Ca V 1.2, however, are incompletely known, but are presumed to require phosphorylation of residues in α 1C and C-terminal proteolytic cleavage of the α 1C subunit. We generated transgenic mice expressing an α 1C with alanine substitutions of all conserved serine or threonine, which is predicted to be a potential PKA phosphorylation site by at least one prediction tool, while sparing the residues previously shown to be phosphorylated but shown individually not to be required for β-adrenergic regulation of Ca V 1.2 current (17-mutant). A second line included these 17 putative sites plus the five previously identified phosphoregulatory sites (22-mutant), thus allowing us to query whether regulation requires their contribution in combination. We determined that acute β-adrenergic regulation does not require any combination of potential PKA phosphorylation sites conserved in human, guinea pig, rabbit, rat, and mouse α 1C subunits. We separately generated transgenic mice with inducible expression of proteolytic-resistant α 1C Prevention of C-terminal cleavage did not alter β-adrenergic stimulation of Ca V 1.2 in the heart. These studies definitively rule out a role for all conserved consensus PKA phosphorylation sites in α 1C in β-adrenergic stimulation of Ca V 1.2, and show that phosphoregulatory sites on α 1C are not redundant and do not each fractionally contribute to the net stimulatory effect of β-adrenergic stimulation. Further, proteolytic cleavage of α 1C is not required for β-adrenergic stimulation of Ca V 1.2.

48 CFR 1437.7001 - Contractor qualification requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Contractor qualification requirements. 1437.7001 Section 1437.7001 Federal Acquisition Regulations System DEPARTMENT OF THE INTERIOR... Contractor qualification requirements. (a) Prior to award of a contract for real property appraisal services...

48 CFR 1004.470 - Investigative Requirements for Contractors.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Investigative Requirements for Contractors. 1004.470 Section 1004.470 Federal Acquisition Regulations System DEPARTMENT OF THE... Investigative Requirements for Contractors. ...

16 CFR § 1512.12 - Requirements for wheel hubs.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 16 Commercial Practices 2 2013-01-01 2013-01-01 false Requirements for wheel hubs. § 1512.12... ACT REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.12 Requirements for wheel hubs. All bicycles (other than sidewalk bicycles) shall meet the following requirements: (a) Locking devices. Wheels...

40 CFR 1036.825 - Reporting and recordkeeping requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE HEAVY-DUTY HIGHWAY ENGINES Definitions... require you to submit written records in an electronic format. (b) The regulations in § 1036.255 and 40... require for engines and equipment regulated under this part: (1) We specify the following requirements...

40 CFR 1036.825 - Reporting and recordkeeping requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE HEAVY-DUTY HIGHWAY ENGINES Definitions... require you to submit written records in an electronic format. (b) The regulations in § 1036.255 and 40... require for engines and equipment regulated under this part: (1) We specify the following requirements...

40 CFR 1036.825 - Reporting and recordkeeping requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

...) AIR POLLUTION CONTROLS CONTROL OF EMISSIONS FROM NEW AND IN-USE HEAVY-DUTY HIGHWAY ENGINES Definitions... require you to submit written records in an electronic format. (b) The regulations in § 1036.255 and 40... require for engines and equipment regulated under this part: (1) We specify the following requirements...

48 CFR 22.1007 - Requirement to obtain wage determinations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Requirement to obtain wage determinations. 22.1007 Section 22.1007 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Amended 22.1007 Requirement to obtain wage determinations. The contracting officer shall obtain wage...

48 CFR 207.471 - Funding requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Funding requirements. 207.471 Section 207.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION PLANNING Equipment Lease or Purchase 207.471 Funding...

48 CFR 207.471 - Funding requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 3 2012-10-01 2012-10-01 false Funding requirements. 207.471 Section 207.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION PLANNING Equipment Lease or Purchase 207.471 Funding...

48 CFR 207.471 - Funding requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Funding requirements. 207.471 Section 207.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION PLANNING Equipment Lease or Purchase 207.471 Funding...

48 CFR 207.471 - Funding requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Funding requirements. 207.471 Section 207.471 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE ACQUISITION PLANNING ACQUISITION PLANNING Equipment Lease or Purchase 207.471 Funding...

24 CFR 200.36 - Financial reporting requirements.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 24 Housing and Urban Development 2 2010-04-01 2010-04-01 false Financial reporting requirements. 200.36 Section 200.36 Housing and Urban Development Regulations Relating to Housing and Urban... Regulations § 200.36 Financial reporting requirements. The mortgagor must comply with the financial reporting...

16 CFR 300.3 - Required label information.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 1 2011-01-01 2011-01-01 false Required label information. 300.3 Section 300.3 Commercial Practices FEDERAL TRADE COMMISSION REGULATIONS UNDER SPECIFIC ACTS OF CONGRESS RULES AND REGULATIONS UNDER THE WOOL PRODUCTS LABELING ACT OF 1939 Labeling § 300.3 Required label...

48 CFR 1646.301 - Contractor inspection requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 6 2010-10-01 2010-10-01 true Contractor inspection requirements. 1646.301 Section 1646.301 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT... Clauses 1646.301 Contractor inspection requirements. The clause set forth at 1652.246-70 shall be inserted...

24 CFR 200.36 - Financial reporting requirements.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 24 Housing and Urban Development 2 2011-04-01 2011-04-01 false Financial reporting requirements. 200.36 Section 200.36 Housing and Urban Development Regulations Relating to Housing and Urban... Regulations § 200.36 Financial reporting requirements. The mortgagor must comply with the financial reporting...

Nontranscriptional regulation of SYK by the coactivator OCA-B is required at multiple stages of B cell development.

PubMed

Siegel, Rachael; Kim, Unkyu; Patke, Alina; Yu, Xin; Ren, Xiaodi; Tarakhovsky, Alexander; Roeder, Robert G

2006-05-19

OCA-B was originally identified as a nuclear transcriptional coactivator that is essential for antigen-driven immune responses. The later identification of a membrane bound, myristoylated form of OCA-B suggested additional, unique functions in B cell signaling pathways. This study has shown that OCA-B also functions in the pre-B1-to-pre-B2 cell transition and, most surprisingly, that it directly interacts with SYK, a tyrosine kinase critical for pre-BCR and BCR signaling. This unprecedented type of interaction-a transcriptional coactivator with a signaling kinase-occurs in the cytoplasm and directly regulates SYK stability. This study indicates that OCA-B is required for pre-BCR and BCR signaling at multiple stages of B cell development through its nontranscriptional regulation of SYK. Combined with the deregulation of OCA-B target genes, this may help explain the multitude of defects observed in B cell development and immune responses of Oca-b-/- mice.

Integration Host Factor Is Required for RpoN-Dependent hrpL Gene Expression and Controls Motility by Positively Regulating rsmB sRNA in Erwinia amylovora.

PubMed

Lee, Jae Hoon; Zhao, Youfu

2016-01-01

Erwinia amylovora requires an hrp-type III secretion system (T3SS) to cause disease. It has been reported that HrpL, the master regulator of T3SS, is transcriptionally regulated by sigma factor 54 (RpoN), YhbH, and HrpS. In this study, the role of integration host factor (IHF) in regulating hrpL and T3SS gene expression was investigated. IHF is a nucleoid-associated protein that regulates gene expression by influencing nucleoid structure and DNA bending. Our results showed that both ihfA and ihfB mutants of E. amylovora did not induce necrotic lesions on pear fruits. Growth of both mutants was greatly reduced, and expression of the hrpL and T3SS genes was significantly down-regulated as compared with those of the wild type. In addition, expression of the ihfA, but not the ihfB gene, was under auto-suppression by IHF. Furthermore, both ihfA and ihfB mutants were hypermotile, due to significantly reduced expression of small RNA (sRNA) rsmB. Electrophoresis mobility shift assay further confirmed that IHF binds to the promoters of the hrpL and ihfA genes, as well as the rsmB sRNA gene. These results indicate that IHF is required for RpoN-dependent hrpL gene expression and virulence, and controls motility by positively regulating the rsmB sRNA in E. amylovora.

16 CFR 1512.12 - Requirements for wheel hubs.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 2 2011-01-01 2011-01-01 false Requirements for wheel hubs. 1512.12 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.12 Requirements for wheel hubs. All bicycles (other... hub retention test, § 1512.18(j)(3), to assure that when the locking devices are released the wheel...

16 CFR 1512.12 - Requirements for wheel hubs.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 2 2010-01-01 2010-01-01 false Requirements for wheel hubs. 1512.12 Section... REGULATIONS REQUIREMENTS FOR BICYCLES Regulations § 1512.12 Requirements for wheel hubs. All bicycles (other... hub retention test, § 1512.18(j)(3), to assure that when the locking devices are released the wheel...

12 CFR 323.3 - Appraisals required; transactions requiring a State certified or licensed appraiser.

Code of Federal Regulations, 2010 CFR

2010-01-01

... advancement of new monies, other than funds necessary to cover reasonable closing costs; (8) The transaction... 12 Banks and Banking 4 2010-01-01 2010-01-01 false Appraisals required; transactions requiring a... CORPORATION REGULATIONS AND STATEMENTS OF GENERAL POLICY APPRAISALS § 323.3 Appraisals required; transactions...

18 CFR 807.1 - Requirement.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 18 Conservation of Power and Water Resources 2 2014-04-01 2014-04-01 false Requirement. 807.1... WITHDRAWAL REGISTRATION § 807.1 Requirement. In addition to any other requirements of Commission regulations, and subject to the consent of the affected member state to this requirement, any person withdrawing or...

18 CFR 807.1 - Requirement.

Code of Federal Regulations, 2010 CFR

2010-04-01

... 18 Conservation of Power and Water Resources 2 2010-04-01 2010-04-01 false Requirement. 807.1... WITHDRAWAL REGISTRATION § 807.1 Requirement. In addition to any other requirements of Commission regulations, and subject to the consent of the affected member state to this requirement, any person withdrawing or...

18 CFR 807.1 - Requirement.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 18 Conservation of Power and Water Resources 2 2011-04-01 2011-04-01 false Requirement. 807.1... WITHDRAWAL REGISTRATION § 807.1 Requirement. In addition to any other requirements of Commission regulations, and subject to the consent of the affected member state to this requirement, any person withdrawing or...

NOVA2-mediated RNA regulation is required for axonal pathfinding during development.

PubMed

Saito, Yuhki; Miranda-Rottmann, Soledad; Ruggiu, Matteo; Park, Christopher Y; Fak, John J; Zhong, Ru; Duncan, Jeremy S; Fabella, Brian A; Junge, Harald J; Chen, Zhe; Araya, Roberto; Fritzsch, Bernd; Hudspeth, A J; Darnell, Robert B

2016-05-25

The neuron specific RNA-binding proteins NOVA1 and NOVA2 are highly homologous alternative splicing regulators. NOVA proteins regulate at least 700 alternative splicing events in vivo, yet relatively little is known about the biologic consequences of NOVA action and in particular about functional differences between NOVA1 and NOVA2. Transcriptome-wide searches for isoform-specific functions, using NOVA1 and NOVA2 specific HITS-CLIP and RNA-seq data from mouse cortex lacking either NOVA isoform, reveals that NOVA2 uniquely regulates alternative splicing events of a series of axon guidance related genes during cortical development. Corresponding axonal pathfinding defects were specific to NOVA2 deficiency: Nova2-/- but not Nova1-/- mice had agenesis of the corpus callosum, and axonal outgrowth defects specific to ventral motoneuron axons and efferent innervation of the cochlea. Thus we have discovered that NOVA2 uniquely regulates alternative splicing of a coordinate set of transcripts encoding key components in cortical, brainstem and spinal axon guidance/outgrowth pathways during neural differentiation, with severe functional consequences in vivo.

48 CFR 231.303 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Requirements. 231.303... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 231.303 Requirements. (1) Pursuant to section 841 of the National Defense...

48 CFR 231.303 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Requirements. 231.303... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 231.303 Requirements. (1) Pursuant to section 841 of the National Defense...

48 CFR 231.303 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 231.303... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 231.303 Requirements. (1) Pursuant to section 841 of the National Defense...

49 CFR 383.117 - Requirements for passenger endorsement.

Code of Federal Regulations, 2010 CFR

2010-10-01

... Section 383.117 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION FEDERAL MOTOR CARRIER SAFETY REGULATIONS COMMERCIAL DRIVER'S LICENSE STANDARDS; REQUIREMENTS AND PENALTIES Required Knowledge and Skills § 383.117...

Intracellular pH regulation in unstimulated Calliphora salivary glands is Na+ dependent and requires V-ATPase activity.

PubMed

Schewe, Bettina; Blenau, Wolfgang; Walz, Bernd

2012-04-15

Salivary gland cells of the blowfly Calliphora vicina have a vacuolar-type H(+)-ATPase (V-ATPase) that lies in their apical membrane and energizes the secretion of a KCl-rich primary saliva upon stimulation with serotonin (5-hydroxytryptamine). Whether and to what extent V-ATPase contributes to intracellular pH (pH(i)) regulation in unstimulated gland cells is unknown. We used the fluorescent dye BCECF to study intracellular pH(i) regulation microfluorometrically and show that: (1) under resting conditions, the application of Na(+)-free physiological saline induces an intracellular alkalinization attributable to the inhibition of the activity of a Na(+)-dependent glutamate transporter; (2) the maintenance of resting pH(i) is Na(+), Cl(-), concanamycin A and DIDS sensitive; (3) recovery from an intracellular acid load is Na(+) sensitive and requires V-ATPase activity; (4) the Na(+)/H(+) antiporter is not involved in pH(i) recovery after a NH(4)Cl prepulse; and (5) at least one Na(+)-dependent transporter and the V-ATPase maintain recovery from an intracellular acid load. Thus, under resting conditions, the V-ATPase and at least one Na(+)-dependent transporter maintain normal pH(i) values of pH 7.5. We have also detected the presence of a Na(+)-dependent glutamate transporter, which seems to act as an acid loader. Despite this not being a common pH(i)-regulating transporter, its activity affects steady-state pH(i) in C. vicina salivary gland cells.

48 CFR 46.202-4 - Higher-level contract quality requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... quality requirements. 46.202-4 Section 46.202-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 46.202-4 Higher-level contract quality requirements. (a) Requiring compliance with higher-level quality standards is appropriate in...

48 CFR 46.202-4 - Higher-level contract quality requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... quality requirements. 46.202-4 Section 46.202-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 46.202-4 Higher-level contract quality requirements. (a) Requiring compliance with higher-level quality standards is appropriate in...

48 CFR 46.202-4 - Higher-level contract quality requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... quality requirements. 46.202-4 Section 46.202-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 46.202-4 Higher-level contract quality requirements. (a) Requiring compliance with higher-level quality standards is appropriate in...

48 CFR 46.202-4 - Higher-level contract quality requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... quality requirements. 46.202-4 Section 46.202-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 46.202-4 Higher-level contract quality requirements. (a) Requiring compliance with higher-level quality standards is appropriate in...

43 CFR 3815.8 - Notation required in application for patent; conditions required in patent.

Code of Federal Regulations, 2012 CFR

2012-10-01

... patent; conditions required in patent. 3815.8 Section 3815.8 Public Lands: Interior Regulations Relating... Notation required in application for patent; conditions required in patent. (a) Every application for patent for any minerals located subject to this Act must bear on its face, before being executed by the...

43 CFR 3815.8 - Notation required in application for patent; conditions required in patent.

Code of Federal Regulations, 2011 CFR

2011-10-01

... patent; conditions required in patent. 3815.8 Section 3815.8 Public Lands: Interior Regulations Relating... Notation required in application for patent; conditions required in patent. (a) Every application for patent for any minerals located subject to this Act must bear on its face, before being executed by the...

43 CFR 3815.8 - Notation required in application for patent; conditions required in patent.

Code of Federal Regulations, 2014 CFR

2014-10-01

... patent; conditions required in patent. 3815.8 Section 3815.8 Public Lands: Interior Regulations Relating... Notation required in application for patent; conditions required in patent. (a) Every application for patent for any minerals located subject to this Act must bear on its face, before being executed by the...

43 CFR 3815.8 - Notation required in application for patent; conditions required in patent.

Code of Federal Regulations, 2013 CFR

2013-10-01

... patent; conditions required in patent. 3815.8 Section 3815.8 Public Lands: Interior Regulations Relating... Notation required in application for patent; conditions required in patent. (a) Every application for patent for any minerals located subject to this Act must bear on its face, before being executed by the...

The French Space Operation Act: Technical Regulations

NASA Astrophysics Data System (ADS)

Trinchero, J. P.; Lazare, B.

2010-09-01

The French Space Operation Act(FSOA) stipulates that a prime objective of the National technical regulations is to protect people, property, public health and the environment. Compliance with these technical regulations is mandatory as of 10 December 2010 for space operations by French space operators and for space operations from French territory. The space safety requirements and regulations governing procedures are based on national and international best practices and experience. A critical design review of the space system and procedures shall be carried out by the applicant, in order to verify compliance with the Technical Regulations. An independent technical assessment of the operation is delegated to CNES. The principles applied when drafting technical regulations are as follows: requirements must as far as possible establish the rules according to the objective to be obtained, rather than how it is to be achieved; requirements must give preference to international standards recognised as being the state of the art; requirements must take previous experience into account. Technical regulations are divided into three sections covering common requirements for the launch, control and return of a space object. A dedicated section will cover specific rules to be applied at the Guiana Space Centre. The main topics addressed by the technical regulations are: operator safety management system; study of risks to people, property, public health and the Earth’s environment; impact study on the outer space environment: space debris generated by the operation; planetary protection.

16 CFR 301.3 - English language requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 16 Commercial Practices 1 2011-01-01 2011-01-01 false English language requirements. 301.3 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.3 English language requirements. All..., shall be set out in the English language. If labels, invoices or advertising matter contain any of the...

16 CFR 301.3 - English language requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 16 Commercial Practices 1 2010-01-01 2010-01-01 false English language requirements. 301.3 Section... AND REGULATIONS UNDER FUR PRODUCTS LABELING ACT Regulations § 301.3 English language requirements. All..., shall be set out in the English language. If labels, invoices or advertising matter contain any of the...

Regulatory Requirements and Technical Analysis for Department of Energy Regulated Performance Assessments of Shallow-Trench Disposal of Low-Level Radioactive Waste at the Nevada Test Site

NASA Astrophysics Data System (ADS)

Crowe, B.; Black, P.; Tauxe, J.; Yucel, V.; Rawlinson, S.; Colarusso, A.; DiSanza, F.

2001-12-01

The National Nuclear Security Administration, Nevada Operations Office (NNSA/NV) operates and maintains two active facilities on the Nevada Test Site (NTS) that dispose Department of Energy (DOE) defense-generated low-level radioactive (LLW), mixed radioactive, and classified waste in shallow trenches, pits and large-diameter boreholes. The operation and maintenance of the LLW disposal sites are self-regulated under DOE Order 435.1, which requires review of a Performance Assessment for four performance objectives: 1) all pathways 25 mrem/yr limit; 2) atmospheric pathways 10 mrem/yr limit; 3) radon flux density of 20 pCi/m2/s; and 4) groundwater resource protection (Safe Drinking Water Act; 4 mrem/yr limit). The inadvertent human intruder is protected under a dual 500- and 100-mrem limit (acute and chronic exposure). In response to the Defense Nuclear Facilities Safety Board Recommendation 92 2, a composite analysis is required that must examine all interacting sources for compliance against both 30 and 100 mrem/yr limits. A small component of classified transuranic waste is buried at intermediate depths in 3-meter diameter boreholes at the Area 5 LLW disposal facility and is assessed through DOE-agreement against the requirements of the Environmental Protection Agency (EPA)'s 40 CFR 191. The hazardous components of mixed LLW are assessed against RCRA requirements. The NTS LLW sites fall directly under three sets of federal regulations and the regulatory differences result not only in organizational challenges, but also in different decision objectives and technical paths to completion. The DOE regulations require deterministic analysis for a 1,000-year compliance assessment supplemented by probabilistic analysis under a long-term maintenance program. The EPA regulations for TRU waste are probabilistically based for a compliance interval of 10,000 years. Multiple steps in the assessments are strongly dependent on assumptions for long-term land use policies

Proposed regs address new hospital tax-exemption requirements.

PubMed

Speizman, Richard A; Moore, V A; Mitchell, Alexandra O

2013-03-01

Proposed regulations set forth detailed rules for implementing the new tax-exemption requirements of Section 501(r) of the Internal Revenue Code for not-for-profit organizations operating hospital facilities. The proposed regulations provide guidance on the written financial assistance policies (FAPs) that hospital facilities are required to establish. The regulations propose methodologies for determining the amounts that a hospital facility can charge FAP-eligible individuals for emergency and other medically necessary care. They prescribe procedures that hospital facilities would be required to follow before engaging in extraordinary collection actions against an individual.

48 CFR 31.303 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Requirements. 31.303... REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 31.303 Requirements. (a) Contracts that refer to this subpart 31.3 for determining allowable costs under contracts...

48 CFR 231.603 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 231.603... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With State, Local, and Federally Recognized Indian Tribal Governments 231.603 Requirements. Under 10 U.S.C...

48 CFR 31.303 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Requirements. 31.303... REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 31.303 Requirements. (a) Contracts that refer to this subpart 31.3 for determining allowable costs under contracts...

48 CFR 231.603 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Requirements. 231.603... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With State, Local, and Federally Recognized Indian Tribal Governments 231.603 Requirements. Under 10 U.S.C...

48 CFR 41.301 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Requirements. 41.301... CONTRACTING ACQUISITION OF UTILITY SERVICES Requests for Assistance 41.301 Requirements. (a) Requests for... utility service requirements, the agency shall furnish the information in paragraph (a) of this section...

48 CFR 41.301 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 1 2014-10-01 2014-10-01 false Requirements. 41.301... CONTRACTING ACQUISITION OF UTILITY SERVICES Requests for Assistance 41.301 Requirements. (a) Requests for... utility service requirements, the agency shall furnish the information in paragraph (a) of this section...

48 CFR 31.303 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 1 2013-10-01 2013-10-01 false Requirements. 31.303... REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 31.303 Requirements. (a) Contracts that refer to this subpart 31.3 for determining allowable costs under contracts...

48 CFR 31.303 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Requirements. 31.303... REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Educational Institutions 31.303 Requirements. (a) Contracts that refer to this subpart 31.3 for determining allowable costs under contracts...

48 CFR 231.603 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Requirements. 231.603... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With State, Local, and Federally Recognized Indian Tribal Governments 231.603 Requirements. Under 10 U.S.C...

48 CFR 231.603 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Requirements. 231.603... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With State, Local, and Federally Recognized Indian Tribal Governments 231.603 Requirements. Under 10 U.S.C...

48 CFR 423.601 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirements. 423.601 Section 423.601 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE...

48 CFR 423.601 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirements. 423.601 Section 423.601 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE...

48 CFR 423.601 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirements. 423.601 Section 423.601 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE...

48 CFR 423.601 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirements. 423.601 Section 423.601 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE...

48 CFR 423.601 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirements. 423.601 Section 423.601 Federal Acquisition Regulations System DEPARTMENT OF AGRICULTURE SOCIOECONOMIC PROGRAMS ENVIRONMENT, ENERGY AND WATER EFFICIENCY, RENEWABLE ENERGY TECHNOLOGIES, OCCUPATIONAL SAFETY, AND DRUG-FREE...

48 CFR 801.602-71 - Basic review requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Basic review requirements... GENERAL DEPARTMENT OF VETERANS AFFAIRS ACQUISITION REGULATION SYSTEM Career Development, Contracting Authority, and Responsibilities 801.602-71 Basic review requirements. Contracting officers must obtain...

48 CFR 53.101 - Requirements for use of forms.

Code of Federal Regulations, 2010 CFR

2010-10-01

... matter applicable to each form is addressed. The specific location of each requirement is identified in... forms. 53.101 Section 53.101 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION (CONTINUED) CLAUSES AND FORMS FORMS General 53.101 Requirements for use of forms. The requirements for use of...

NOVA2-mediated RNA regulation is required for axonal pathfinding during development

PubMed Central

Saito, Yuhki; Miranda-Rottmann, Soledad; Ruggiu, Matteo; Park, Christopher Y; Fak, John J; Zhong, Ru; Duncan, Jeremy S; Fabella, Brian A; Junge, Harald J; Chen, Zhe; Araya, Roberto; Fritzsch, Bernd; Hudspeth, A J; Darnell, Robert B

2016-01-01

The neuron specific RNA-binding proteins NOVA1 and NOVA2 are highly homologous alternative splicing regulators. NOVA proteins regulate at least 700 alternative splicing events in vivo, yet relatively little is known about the biologic consequences of NOVA action and in particular about functional differences between NOVA1 and NOVA2. Transcriptome-wide searches for isoform-specific functions, using NOVA1 and NOVA2 specific HITS-CLIP and RNA-seq data from mouse cortex lacking either NOVA isoform, reveals that NOVA2 uniquely regulates alternative splicing events of a series of axon guidance related genes during cortical development. Corresponding axonal pathfinding defects were specific to NOVA2 deficiency: Nova2-/- but not Nova1-/- mice had agenesis of the corpus callosum, and axonal outgrowth defects specific to ventral motoneuron axons and efferent innervation of the cochlea. Thus we have discovered that NOVA2 uniquely regulates alternative splicing of a coordinate set of transcripts encoding key components in cortical, brainstem and spinal axon guidance/outgrowth pathways during neural differentiation, with severe functional consequences in vivo. DOI: http://dx.doi.org/10.7554/eLife.14371.001 PMID:27223325

24 CFR 200.620 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-04-01

... 24 Housing and Urban Development 2 2014-04-01 2014-04-01 false Requirements. 200.620 Section 200... Requirements. With respect to all FHA subsidized or unsubsidized programs in which the applicant hereafter... of these regulations), the applicant shall meet the following requirements or, if he contracts...

24 CFR 200.620 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 24 Housing and Urban Development 2 2012-04-01 2012-04-01 false Requirements. 200.620 Section 200... Requirements. With respect to all FHA subsidized or unsubsidized programs in which the applicant hereafter... of these regulations), the applicant shall meet the following requirements or, if he contracts...

48 CFR 806.570 - Planning requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 5 2010-10-01 2010-10-01 false Planning requirements. 806... AND ACQUISITION PLANNING COMPETITION REQUIREMENTS Competition Advocates 806.570 Planning requirements... planning that is required. (3) A description of how to integrate the Competition Plan into advance...

48 CFR 242.7302 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... Section 242.7302 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT CONTRACT ADMINISTRATION AND AUDIT SERVICES Contractor Insurance/Pension Review 242.7302 Requirements. (a)(1) An in-depth CIPR as described at DFARS 242.7301(a)(1) shall...

49 CFR 1220.5 - Waiver of requirements of these regulations.

Code of Federal Regulations, 2010 CFR

2010-10-01

... would impose an unreasonable burden on the company. ... regulations may be made by the Board upon its own initiative or upon submission of a written request by the company. Each request for waiver shall demonstrate that unusual circumstances warrant a departure from...

Environmentally regulated aerospace coatings

NASA Technical Reports Server (NTRS)

Morris, Virginia L.

1995-01-01

Aerospace coatings represent a complex technology which must meet stringent performance requirements in the protection of aerospace vehicles. Topcoats and primers are used, primarily, to protect the structural elements of the air vehicle from exposure to and subsequent degradation by environmental elements. There are also many coatings which perform special functions, i.e., chafing resistance, rain erosion resistance, radiation and electric effects, fuel tank coatings, maskants, wire and fastener coatings. The scheduled promulgation of federal environmental regulations for aerospace manufacture and rework materials and processes will regulate the emissions of photochemically reactive precursors to smog and air toxics. Aerospace organizations will be required to identify, qualify and implement less polluting materials. The elimination of ozone depleting chemicals (ODC's) and implementation of pollution prevention requirements are added constraints which must be addressed concurrently. The broad categories of operations affected are the manufacture, operation, maintenance, and repair of military, commercial, general aviation, and space vehicles. The federal aerospace regulations were developed around the precept that technology had to be available to support the reduction of organic and air toxic emissions, i.e., the regulations cannot be technology forcing. In many cases, the regulations which are currently in effect in the South Coast Air Quality Management District (SCAQMD), located in Southern California, were used as the baseline for the federal regulations. This paper addresses strategies used by Southern California aerospace organizations to cope with these regulatory impacts on aerospace productions programs. All of these regulatory changes are scheduled for implementation in 1993 and 1994, with varying compliance dates established.

48 CFR 370.503 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirements. 370.503... SPECIAL PROGRAMS AFFECTING ACQUISITION Acquisitions Under the Buy Indian Act 370.503 Requirements. (a.... Contracts awarded under the Buy Indian Act are subject to the requirements of section 7(b) of the Indian...

23 CFR 500.105 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-04-01

... 23 Highways 1 2012-04-01 2012-04-01 false Requirements. 500.105 Section 500.105 Highways FEDERAL... AND MONITORING SYSTEMS Management Systems § 500.105 Requirements. (a) The metropolitan transportation... requirements of § 500.109 of this regulation. (b) States shall develop, establish, and implement a TMS that...

48 CFR 231.703 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 231.703... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 231.703 Requirements. Under 10 U.S.C. 2249, the costs cited in 231.205-22(a) are...

48 CFR 231.703 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Requirements. 231.703... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 231.703 Requirements. Under 10 U.S.C. 2249, the costs cited in 231.205-22(a) are...

48 CFR 231.703 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Requirements. 231.703... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 231.703 Requirements. Under 10 U.S.C. 2249, the costs cited in 231.205-22(a) are...

23 CFR 500.105 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-04-01

... 23 Highways 1 2011-04-01 2011-04-01 false Requirements. 500.105 Section 500.105 Highways FEDERAL... AND MONITORING SYSTEMS Management Systems § 500.105 Requirements. (a) The metropolitan transportation... requirements of § 500.109 of this regulation. (b) States shall develop, establish, and implement a TMS that...

48 CFR 370.503 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirements. 370.503... SPECIAL PROGRAMS AFFECTING ACQUISITION Acquisitions Under the Buy Indian Act 370.503 Requirements. (a.... Contracts awarded under the Buy Indian Act are subject to the requirements of section 7(b) of the Indian...

48 CFR 370.503 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirements. 370.503... SPECIAL PROGRAMS AFFECTING ACQUISITION Acquisitions Under the Buy Indian Act 370.503 Requirements. (a.... Contracts awarded under the Buy Indian Act are subject to the requirements of section 7(b) of the Indian...

48 CFR 370.503 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirements. 370.503... SPECIAL PROGRAMS AFFECTING ACQUISITION Acquisitions Under the Buy Indian Act 370.503 Requirements. (a.... Contracts awarded under the Buy Indian Act are subject to the requirements of section 7(b) of the Indian...

48 CFR 370.503 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirements. 370.503... SPECIAL PROGRAMS AFFECTING ACQUISITION Acquisitions Under the Buy Indian Act 370.503 Requirements. (a.... Contracts awarded under the Buy Indian Act are subject to the requirements of section 7(b) of the Indian...

48 CFR 231.703 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Requirements. 231.703... OF DEFENSE GENERAL CONTRACTING REQUIREMENTS CONTRACT COST PRINCIPLES AND PROCEDURES Contracts With Nonprofit Organizations 231.703 Requirements. Under 10 U.S.C. 2249, the costs cited in 231.205-22(a) are...

Financial Management Regulation. Volume 1. General Financial Management Information, Systems, and Requirements

DTIC Science & Technology

1993-05-01

20301-1100 DoD Components will be provided copies of this Volume of the Regulation through normal publication channels. Other Federal Agencies and the... public may obtain copies of this Volume from the National Technical Information Service, 5285 Port Royal Road, Springfield, VA 22161, (703) 487- 4650...SECTION 1 PARAGRAPH 1 SUtHI’ARAGRAIlTS ii DoD Financial Management Regulation Volume 1, Introductionp PUBLICATIONS SUPERSEDED This Volume of the

48 CFR 28.101-4 - Noncompliance with bid guarantee requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Noncompliance with bid guarantee requirements. 28.101-4 Section 28.101-4 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION GENERAL CONTRACTING REQUIREMENTS BONDS AND INSURANCE Bonds and Other Financial Protections 28.101...

48 CFR 617.503 - Determination and findings requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Determination and findings requirements. 617.503 Section 617.503 Federal Acquisition Regulations System DEPARTMENT OF STATE CONTRACTING....503 Determination and findings requirements. The authority to make the determination prescribed in FAR...

48 CFR 1646.301 - Contractor inspection requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false Contractor inspection requirements. 1646.301 Section 1646.301 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract...

48 CFR 1646.301 - Contractor inspection requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 6 2011-10-01 2011-10-01 false Contractor inspection requirements. 1646.301 Section 1646.301 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract...

48 CFR 1646.301 - Contractor inspection requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false Contractor inspection requirements. 1646.301 Section 1646.301 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract...

48 CFR 1646.301 - Contractor inspection requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false Contractor inspection requirements. 1646.301 Section 1646.301 Federal Acquisition Regulations System OFFICE OF PERSONNEL MANAGEMENT FEDERAL EMPLOYEES HEALTH BENEFITS ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract...

48 CFR 970.3002 - CAS program requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 5 2011-10-01 2011-10-01 false CAS program requirements. 970.3002 Section 970.3002 Federal Acquisition Regulations System DEPARTMENT OF ENERGY AGENCY SUPPLEMENTARY REGULATIONS DOE MANAGEMENT AND OPERATING CONTRACTS Cost Accounting Standards Administration 970...

Up-regulation of insulin-like growth factor 2 by ketamine requires glycogen synthase kinase-3 inhibition.

PubMed

Grieco, Steven F; Cheng, Yuyan; Eldar-Finkelman, Hagit; Jope, Richard S; Beurel, Eléonore

2017-01-04

An antidepressant dose of the rapidly-acting ketamine inhibits glycogen synthase kinase-3 (GSK3) in mouse hippocampus, and this inhibition is required for the antidepressant effect of ketamine in learned helplessness depression-like behavior. Here we report that treatment with an antidepressant dose of ketamine (10mg/kg) increased expression of insulin-like growth factor 2 (IGF2) in mouse hippocampus, an effect that required ketamine-induced inhibition of GSK3. Ketamine also inhibited hippocampal GSK3 and increased expression of hippocampal IGF2 in mice when administered after the induction of learned helplessness. Treatment with the specific GSK3 inhibitor L803-mts was sufficient to up-regulate hippocampal IGF2 expression. Administration of IGF2 siRNA reduced ketamine's antidepressant effect in the learned helplessness paradigm. Mice subjected to the learned helplessness paradigm were separated into two groups, those that were resilient (non-depressed) and those that were susceptible (depressed). Non-depressed resilient mice displayed higher expression of IGF2 than susceptible mice. These results indicate that IGF2 contributes to ketamine's antidepressant effect and that IGF2 may confer resilience to depression-like behavior. Copyright © 2016 Elsevier Inc. All rights reserved.

Up-regulation of insulin-like growth factor 2 by ketamine requires glycogen synthase kinase-3 inhibition

PubMed Central

Grieco, Steven F.; Cheng, Yuyan; Eldar-Finkelman, Hagit; Jope, Richard S.; Beurel, Eléonore

2016-01-01

An antidepressant dose of the rapidly-acting ketamine inhibits glycogen synthase kinase-3 (GSK3) in mouse hippocampus, and this inhibition is required for the antidepressant effect of ketamine in learned helplessness depression-like behavior. Here we report that treatment with an antidepressant dose of ketamine (10 mg/kg) increased expression of insulin-like growth factor 2 (IGF2) in mouse hippocampus, an effect that required ketamine-induced inhibition of GSK3. Ketamine also inhibited hippocampal GSK3 and increased expression of hippocampal IGF2 in mice when administered after the induction of learned helplessness. Treatment with the specific GSK3 inhibitor L803-mts was sufficient to up-regulate hippocampal IGF2 expression. Administration of IGF2 siRNA reduced ketamine's antidepressant effect in the learned helplessness paradigm. Mice subjected to the learned helplessness paradigm were separated into two groups, those that were resilient (non-depressed) and those that were susceptible (depressed). Non-depressed resilient mice displayed higher expression of IGF2 than susceptible mice. These results indicate that IGF2 contributes to ketamine's antidepressant effect and that IGF2 may confer resilience to depression-like behavior. PMID:27542584

48 CFR 246.202-4 - Higher-level contract quality requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... quality requirements. 246.202-4 Section 246.202-4 Federal Acquisition Regulations System DEFENSE ACQUISITION REGULATIONS SYSTEM, DEPARTMENT OF DEFENSE CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 246.202-4 Higher-level contract quality requirements. (1) Higher-level contract quality...

49 CFR 565.15 - Content requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 6 2012-10-01 2012-10-01 false Content requirements. 565.15 Section 565.15 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements § 565.15 Content requirements. (a) The...

49 CFR 565.15 - Content requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 6 2014-10-01 2014-10-01 false Content requirements. 565.15 Section 565.15 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements § 565.15 Content requirements. (a) The...

49 CFR 565.15 - Content requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 6 2013-10-01 2013-10-01 false Content requirements. 565.15 Section 565.15 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION VEHICLE IDENTIFICATION NUMBER (VIN) REQUIREMENTS VIN Requirements § 565.15 Content requirements. (a) The...

49 CFR 383.111 - Required knowledge.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 5 2011-10-01 2011-10-01 false Required knowledge. 383.111 Section 383.111... STANDARDS; REQUIREMENTS AND PENALTIES Required Knowledge and Skills § 383.111 Required knowledge. (a) All CMV operators must have knowledge of the following 20 general areas: (1) Safe operations regulations...

78 FR 25818 - Truth in Lending (Regulation Z)

Federal Register 2010, 2011, 2012, 2013, 2014

2013-05-03

...The Bureau of Consumer Financial Protection (Bureau) issues this final rule to amend Regulation Z, which implements the Truth in Lending Act (TILA), and the official interpretations to the regulation. Regulation Z generally prohibits a card issuer from opening a credit card account for a consumer, or increasing the credit limit applicable to a credit card account, unless the card issuer considers the consumer's ability to make the required payments under the terms of such account. Regulation Z currently requires that issuers consider the consumer's independent ability to pay, regardless of the consumer's age; in contrast, TILA expressly requires consideration of an independent ability to pay only for applicants who are under the age of 21. The final rule amends Regulation Z to remove the requirement that issuers consider the consumer's independent ability to pay for applicants who are 21 or older, and permits issuers to consider income and assets to which such consumers have a reasonable expectation of access.

40 CFR 141.132 - Monitoring requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Disinfectant Residuals, Disinfection Byproducts, and Disinfection Byproduct Precursors § 141.132 Monitoring requirements. (a) General requirements. (1) Systems must... this subpart to qualify for reduced monitoring. (b) Monitoring requirements for disinfection byproducts...

40 CFR 141.132 - Monitoring requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Disinfectant Residuals, Disinfection Byproducts, and Disinfection Byproduct Precursors § 141.132 Monitoring requirements. (a) General requirements. (1) Systems must... this subpart to qualify for reduced monitoring. (b) Monitoring requirements for disinfection byproducts...

40 CFR 141.132 - Monitoring requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... (CONTINUED) NATIONAL PRIMARY DRINKING WATER REGULATIONS Disinfectant Residuals, Disinfection Byproducts, and Disinfection Byproduct Precursors § 141.132 Monitoring requirements. (a) General requirements. (1) Systems must... this subpart to qualify for reduced monitoring. (b) Monitoring requirements for disinfection byproducts...

Discovery of a Splicing Regulator Required for Cell Cycle Progression

DOE Office of Scientific and Technical Information (OSTI.GOV)

Suvorova, Elena S.; Croken, Matthew; Kratzer, Stella

2013-02-01

In the G1 phase of the cell division cycle, eukaryotic cells prepare many of the resources necessary for a new round of growth including renewal of the transcriptional and protein synthetic capacities and building the machinery for chromosome replication. The function of G1 has an early evolutionary origin and is preserved in single and multicellular organisms, although the regulatory mechanisms conducting G1 specific functions are only understood in a few model eukaryotes. Here we describe a new G1 mutant from an ancient family of apicomplexan protozoans. Toxoplasma gondii temperature-sensitive mutant 12-109C6 conditionally arrests in the G1 phase due to amore » single point mutation in a novel protein containing a single RNA-recognition-motif (TgRRM1). The resulting tyrosine to asparagine amino acid change in TgRRM1 causes severe temperature instability that generates an effective null phenotype for this protein when the mutant is shifted to the restrictive temperature. Orthologs of TgRRM1 are widely conserved in diverse eukaryote lineages, and the human counterpart (RBM42) can functionally replace the missing Toxoplasma factor. Transcriptome studies demonstrate that gene expression is downregulated in the mutant at the restrictive temperature due to a severe defect in splicing that affects both cell cycle and constitutively expressed mRNAs. The interaction of TgRRM1 with factors of the tri-SNP complex (U4/U6 & U5 snRNPs) indicate this factor may be required to assemble an active spliceosome. Thus, the TgRRM1 family of proteins is an unrecognized and evolutionarily conserved class of splicing regulators. This study demonstrates investigations into diverse unicellular eukaryotes, like the Apicomplexa, have the potential to yield new insights into important mechanisms conserved across modern eukaryotic kingdoms.« less

48 CFR 317.503 - Determination and findings requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Determination and findings requirements. 317.503 Section 317.503 Federal Acquisition Regulations System HEALTH AND HUMAN SERVICES... Act 317.503 Determination and findings requirements. (a) In addition to the D & F contents specified...

29 CFR 36.125 - Effect of other requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 36.125 Effect of other requirements. (a) Effect of other Federal provisions. The obligations imposed by these Title IX regulations are independent... Act of Congress or Federal regulation. (b) Effect of State or local law or other requirements. The...

10 CFR 1042.125 - Effect of other requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... PROGRAMS OR ACTIVITIES RECEIVING FEDERAL FINANCIAL ASSISTANCE Introduction § 1042.125 Effect of other requirements. (a) Effect of other Federal provisions. The obligations imposed by these Title IX regulations are... Act of Congress or Federal regulation. (b) Effect of State or local law or other requirements. The...

Dimeric c-di-GMP is required for post-translational regulation of alginate production in Pseudomonas aeruginosa

DOE PAGES

Whitney, John C.; Robinson, Howard; Whitfield, Gregory B.; ...

2015-05-15

Pseudomonas aeruginosa is an opportunistic human pathogen that secretes the exopolysaccharide alginate during infection of the respiratory tract of individuals afflicted with cystic fibrosis and chronic obstructive pulmonary disease. Among the proteins required for alginate production, Alg44 has been identified as an inner membrane protein whose bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) binding activity post-translationally regulates alginate secretion. In this study, we report the 1.8 Å crystal structure of the cytoplasmic region of Alg44 in complex with dimeric self-intercalated c-di-GMP and characterize its dinucleotide-binding site using mutational analysis. The structure shows that the c-di-GMP binding region of Alg44 adopts a PilZmore » domain fold with a dimerization mode not previously observed for this family of proteins. Moreover, calorimetric binding analysis of residues in the c-di-GMP binding site demonstrate that mutation of Arg-17 and Arg-95 alters the binding stoichiometry between c-di-GMP and Alg44 from 2:1 to 1:1. Introduction of these mutant alleles on the P. aeruginosa chromosome show that the residues required for binding of dimeric c-di-GMP in vitro are also required for efficient alginate production in vivo. Our results suggest that the dimeric form of c-di-GMP represents the biologically active signaling molecule needed for the secretion of an important virulence factor produced by P. aeruginosa.« less

Dimeric c-di-GMP is required for post-translational regulation of alginate production in Pseudomonas aeruginosa

DOE Office of Scientific and Technical Information (OSTI.GOV)

Whitney, John C.; Robinson, Howard; Whitfield, Gregory B.

Pseudomonas aeruginosa is an opportunistic human pathogen that secretes the exopolysaccharide alginate during infection of the respiratory tract of individuals afflicted with cystic fibrosis and chronic obstructive pulmonary disease. Among the proteins required for alginate production, Alg44 has been identified as an inner membrane protein whose bis-(3',5')-cyclic dimeric guanosine monophosphate (c-di-GMP) binding activity post-translationally regulates alginate secretion. In this study, we report the 1.8 Å crystal structure of the cytoplasmic region of Alg44 in complex with dimeric self-intercalated c-di-GMP and characterize its dinucleotide-binding site using mutational analysis. The structure shows that the c-di-GMP binding region of Alg44 adopts a PilZmore » domain fold with a dimerization mode not previously observed for this family of proteins. Moreover, calorimetric binding analysis of residues in the c-di-GMP binding site demonstrate that mutation of Arg-17 and Arg-95 alters the binding stoichiometry between c-di-GMP and Alg44 from 2:1 to 1:1. Introduction of these mutant alleles on the P. aeruginosa chromosome show that the residues required for binding of dimeric c-di-GMP in vitro are also required for efficient alginate production in vivo. Our results suggest that the dimeric form of c-di-GMP represents the biologically active signaling molecule needed for the secretion of an important virulence factor produced by P. aeruginosa.« less

Dimeric c-di-GMP Is Required for Post-translational Regulation of Alginate Production in Pseudomonas aeruginosa*

PubMed Central

Whitney, John C.; Whitfield, Gregory B.; Marmont, Lindsey S.; Yip, Patrick; Neculai, A. Mirela; Lobsanov, Yuri D.; Robinson, Howard; Ohman, Dennis E.; Howell, P. Lynne

2015-01-01

Pseudomonas aeruginosa is an opportunistic human pathogen that secretes the exopolysaccharide alginate during infection of the respiratory tract of individuals afflicted with cystic fibrosis and chronic obstructive pulmonary disease. Among the proteins required for alginate production, Alg44 has been identified as an inner membrane protein whose bis-(3′,5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) binding activity post-translationally regulates alginate secretion. In this study, we report the 1.8 Å crystal structure of the cytoplasmic region of Alg44 in complex with dimeric self-intercalated c-di-GMP and characterize its dinucleotide-binding site using mutational analysis. The structure shows that the c-di-GMP binding region of Alg44 adopts a PilZ domain fold with a dimerization mode not previously observed for this family of proteins. Calorimetric binding analysis of residues in the c-di-GMP binding site demonstrate that mutation of Arg-17 and Arg-95 alters the binding stoichiometry between c-di-GMP and Alg44 from 2:1 to 1:1. Introduction of these mutant alleles on the P. aeruginosa chromosome show that the residues required for binding of dimeric c-di-GMP in vitro are also required for efficient alginate production in vivo. These results suggest that the dimeric form of c-di-GMP represents the biologically active signaling molecule needed for the secretion of an important virulence factor produced by P. aeruginosa. PMID:25817996

76 FR 26802 - Application Filing Requirements

Federal Register 2010, 2011, 2012, 2013, 2014

2011-05-09

... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application Filing Requirements AGENCY... concerning the following information collection. Title of Proposal: Application Filing Requirements. OMB Number: 1550-0056. Form Number: N/A. Description: OTS regulations require that applications, notices, or...

76 FR 41331 - Application Filing Requirements

Federal Register 2010, 2011, 2012, 2013, 2014

2011-07-13

... DEPARTMENT OF THE TREASURY Office of Thrift Supervision Application Filing Requirements AGENCY... following information collection. Title of Proposal: Application Filing Requirements. OMB Number: 1550-0056. Form Number: N/A. Description: OTS regulations require that applications, notices, or other filings...

48 CFR 46.202-4 - Higher-level contract quality requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Higher-level contract... REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 46.202-4 Higher-level contract quality requirements. (a) Requiring compliance with higher-level quality standards is appropriate in...

48 CFR 306.303-1 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 4 2014-10-01 2014-10-01 false Requirements. 306.303-1... ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 306.303-1 Requirements. (b... written in a manner to permit an individual without technical knowledge of the requirement to understand...

48 CFR 6.303-1 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 1 2011-10-01 2011-10-01 false Requirements. 6.303-1... COMPETITION REQUIREMENTS Other Than Full and Open Competition 6.303-1 Requirements. (a) A contracting officer... are made public after award in accordance with 6.305. (c) Technical and requirements personnel are...

48 CFR 306.303-1 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 4 2013-10-01 2013-10-01 false Requirements. 306.303-1... ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 306.303-1 Requirements. (b... written in a manner to permit an individual without technical knowledge of the requirement to understand...

48 CFR 306.303-1 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 4 2012-10-01 2012-10-01 false Requirements. 306.303-1... ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 306.303-1 Requirements. (b... written in a manner to permit an individual without technical knowledge of the requirement to understand...

48 CFR 306.303-1 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 4 2010-10-01 2010-10-01 false Requirements. 306.303-1... ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 306.303-1 Requirements. (b... written in a manner to permit an individual without technical knowledge of the requirement to understand...

48 CFR 306.303-1 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 4 2011-10-01 2011-10-01 false Requirements. 306.303-1... ACQUISITION PLANNING COMPETITION REQUIREMENTS Other Than Full and Open Competition 306.303-1 Requirements. (b... written in a manner to permit an individual without technical knowledge of the requirement to understand...

7 CFR 46.3 - License required.

Code of Federal Regulations, 2010 CFR

2010-01-01

... Regulations of the Department of Agriculture AGRICULTURAL MARKETING SERVICE (Standards, Inspections, Marketing Practices), DEPARTMENT OF AGRICULTURE MARKETING OF PERISHABLE AGRICULTURAL COMMODITIES REGULATIONS (OTHER... considered to result in separate firms and, therefore, do not require separate licenses. ...

Temporal requirements of the fragile X mental retardation protein in the regulation of synaptic structure.

PubMed

Gatto, Cheryl L; Broadie, Kendal

2008-08-01

Fragile X syndrome (FraX), caused by the loss-of-function of one gene (FMR1), is the most common inherited form of both mental retardation and autism spectrum disorders. The FMR1 product (FMRP) is an mRNA-binding translation regulator that mediates activity-dependent control of synaptic structure and function. To develop any FraX intervention strategy, it is essential to define when and where FMRP loss causes the manifestation of synaptic defects, and whether the reintroduction of FMRP can restore normal synapse properties. In the Drosophila FraX model, dFMRP loss causes neuromuscular junction (NMJ) synapse over-elaboration (overgrowth, overbranching, excess synaptic boutons), accumulation of development-arrested satellite boutons, and altered neurotransmission. We used the Gene-Switch method to conditionally drive dFMRP expression to define the spatiotemporal requirements in synaptic mechanisms. Constitutive induction of targeted neuronal dFMRP at wild-type levels rescues all synaptic architectural defects in Drosophila Fmr1 (dfmr1)-null mutants, demonstrating a presynaptic requirement for synapse structuring. By contrast, presynaptic dFMRP expression does not ameliorate functional neurotransmission defects, indicating a postsynaptic dFMRP requirement. Strikingly, targeted early induction of dFMRP effects nearly complete rescue of synaptic structure defects, showing a primarily early-development role. In addition, acute dFMRP expression at maturity partially alleviates dfmr1-null defects, although rescue is not as complete as either early or constitutive dFMRP expression, showing a modest capacity for late-stage structural plasticity. We conclude that dFMRP predominantly acts early in synaptogenesis to modulate architecture, but that late dFMRP introduction at maturity can weakly compensate for early absence of dFMRP function.

29 CFR 548.200 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-07-01

... 29 Labor 3 2014-07-01 2014-07-01 false Requirements. 548.200 Section 548.200 Labor Regulations... ESTABLISHED BASIC RATES FOR COMPUTING OVERTIME PAY Interpretations Requirements for A Basic Rate § 548.200 Requirements. The following conditions must be satisfied if a “basic” rate is to be considered proper under...

29 CFR 548.200 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-07-01

... 29 Labor 3 2013-07-01 2013-07-01 false Requirements. 548.200 Section 548.200 Labor Regulations... ESTABLISHED BASIC RATES FOR COMPUTING OVERTIME PAY Interpretations Requirements for A Basic Rate § 548.200 Requirements. The following conditions must be satisfied if a “basic” rate is to be considered proper under...

29 CFR 548.200 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-07-01

... 29 Labor 3 2012-07-01 2012-07-01 false Requirements. 548.200 Section 548.200 Labor Regulations... ESTABLISHED BASIC RATES FOR COMPUTING OVERTIME PAY Interpretations Requirements for A Basic Rate § 548.200 Requirements. The following conditions must be satisfied if a “basic” rate is to be considered proper under...

Regulating chemicals: law, science, and the unbearable burdens of regulation.

PubMed

Silbergeld, Ellen K; Mandrioli, Daniele; Cranor, Carl F

2015-03-18

The challenges of regulating industrial chemicals remain unresolved in the United States. The Toxic Substances Control Act (TSCA) of 1976 was the first legislation to extend coverage to the regulation of industrial chemicals, both existing and newly registered. However, decisions related to both law and science that were made in passing this law inevitably rendered it ineffectual. Attempts to fix these shortcomings have not been successful. In light of the European Union's passage of innovative principles and requirements for chemical regulation, it is no longer possible to deny the opportunity and need for reform in US law and practice.

48 CFR 52.204-2 - Security Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Security Requirements. 52.204-2 Section 52.204-2 Federal Acquisition Regulations System FEDERAL ACQUISITION REGULATION... Contractor agrees to insert terms that conform substantially to the language of this clause, including this...

43 CFR 3453.2 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 43 Public Lands: Interior 2 2012-10-01 2012-10-01 false Requirements. 3453.2 Section 3453.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT..., Sublease or Otherwise § 3453.2 Requirements. ...

43 CFR 3453.2 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 43 Public Lands: Interior 2 2013-10-01 2013-10-01 false Requirements. 3453.2 Section 3453.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT..., Sublease or Otherwise § 3453.2 Requirements. ...

43 CFR 3453.2 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 43 Public Lands: Interior 2 2014-10-01 2014-10-01 false Requirements. 3453.2 Section 3453.2 Public Lands: Interior Regulations Relating to Public Lands (Continued) BUREAU OF LAND MANAGEMENT, DEPARTMENT..., Sublease or Otherwise § 3453.2 Requirements. ...

H. R. 2587: A bill to amend the Solid Waste Disposal Act to authorize States to regulate the interstate transportation of solid waste, to authorize a private right of action to enforce any such regulation, and to require any such regulation to include certain civil and criminal penalties, introduced in the US House of Representatives, One Hundred Second Congress, First Session, June 7, 1991

DOE Office of Scientific and Technical Information (OSTI.GOV)

Not Available

1991-01-01

The bill was introduced into the US House of Representatives on June 7, 1991 to amend the Solid Waste Disposal Act. This legislation authorizes states to regulate the interstate transportation of solid waste, and to authorize a private right of action to enforce any such regulation. The key feature on this bill focuses on authorizing states to regulate solid waste in interstate commerce. In addition, any regulation is required to include certain civil and criminal penalties. This act requires that a civil penalty of a fine not to exceed $1,000,000 and a criminal penalty of a fine not to exceedmore » $1,000,000, or imprisonment for not more than 1 year, or both.« less

48 CFR 252.237-7003 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 252.237-7003... Clauses 252.237-7003 Requirements. As prescribed in 237.7003(b), use the following clause: Requirements... from the Contractor all of its requirements in the area of performance for the supplies and services...

48 CFR 52.216-21 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 48 Federal Acquisition Regulations System 2 2012-10-01 2012-10-01 false Requirements. 52.216-21... Requirements. As prescribed in 16.506(d), insert the following clause: Requirements (OCT 1995) (a) This is a requirements contract for the supplies or services specified, and effective for the period stated, in the...

48 CFR 52.216-21 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 2 2014-10-01 2014-10-01 false Requirements. 52.216-21... Requirements. As prescribed in 16.506(d), insert the following clause: Requirements (OCT 1995) (a) This is a requirements contract for the supplies or services specified, and effective for the period stated, in the...

48 CFR 252.237-7003 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 3 2011-10-01 2011-10-01 false Requirements. 252.237-7003... Clauses 252.237-7003 Requirements. As prescribed in 237.7003(b), use the following clause: Requirements... from the Contractor all of its requirements in the area of performance for the supplies and services...

48 CFR 52.216-21 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 2 2010-10-01 2010-10-01 false Requirements. 52.216-21... Requirements. As prescribed in 16.506(d), insert the following clause: Requirements (OCT 1995) (a) This is a requirements contract for the supplies or services specified, and effective for the period stated, in the...

48 CFR 52.216-21 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 48 Federal Acquisition Regulations System 2 2011-10-01 2011-10-01 false Requirements. 52.216-21... Requirements. As prescribed in 16.506(d), insert the following clause: Requirements (OCT 1995) (a) This is a requirements contract for the supplies or services specified, and effective for the period stated, in the...

48 CFR 252.237-7003 - Requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 48 Federal Acquisition Regulations System 3 2010-10-01 2010-10-01 false Requirements. 252.237-7003... Clauses 252.237-7003 Requirements. As prescribed in 237.7003(b), use the following clause: Requirements... from the Contractor all of its requirements in the area of performance for the supplies and services...

48 CFR 252.237-7003 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Requirements. 252.237-7003... Clauses 252.237-7003 Requirements. As prescribed in 237.7003(b), use the following clause: Requirements... from the Contractor all of its requirements in the area of performance for the supplies and services...

48 CFR 242.7302 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 48 Federal Acquisition Regulations System 3 2014-10-01 2014-10-01 false Requirements. 242.7302.../Pension Review 242.7302 Requirements. (a)(1) An in-depth CIPR as described at DFARS 242.7301(a)(1) shall... requirements in paragraph (a) of this section) when any of the following circumstances exists, but only if the...

48 CFR 242.7302 - Requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 48 Federal Acquisition Regulations System 3 2013-10-01 2013-10-01 false Requirements. 242.7302.../Pension Review 242.7302 Requirements. (a)(1) An in-depth CIPR as described at DFARS 242.7301(a)(1) shall... requirements in paragraph (a) of this section) when any of the following circumstances exists, but only if the...

36 CFR 1211.125 - Effect of other requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... FEDERAL FINANCIAL ASSISTANCE Introduction § 1211.125 Effect of other requirements. (a) Effect of other... regulation. (b) Effect of State or local law or other requirements. The obligation to comply with these Title..., on the basis of sex, to practice any occupation or profession. (c) Effect of rules or regulations of...

32 CFR 196.125 - Effect of other requirements.

Code of Federal Regulations, 2011 CFR

2011-07-01

... FEDERAL FINANCIAL ASSISTANCE Introduction § 196.125 Effect of other requirements. (a) Effect of other... regulation. (b) Effect of State or local law or other requirements. The obligation to comply with these Title..., on the basis of sex, to practice any occupation or profession. (c) Effect of rules or regulations of...

24 CFR 3.125 - Effect of other requirements.

Code of Federal Regulations, 2011 CFR

2011-04-01

... FINANCIAL ASSISTANCE Introduction § 3.125 Effect of other requirements. (a) Effect of other Federal... regulation. (b) Effect of State or local law or other requirements. The obligation to comply with these Title..., on the basis of sex, to practice any occupation or profession. (c) Effect of rules or regulations of...

7 CFR 3575.9 - Environmental requirements.

Code of Federal Regulations, 2011 CFR

2011-01-01

... 7 Agriculture 15 2011-01-01 2011-01-01 false Environmental requirements. 3575.9 Section 3575.9 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE GENERAL Community Programs Guaranteed Loans § 3575.9 Environmental requirements. Requirements for...

7 CFR 3575.9 - Environmental requirements.

Code of Federal Regulations, 2012 CFR

2012-01-01

... 7 Agriculture 15 2012-01-01 2012-01-01 false Environmental requirements. 3575.9 Section 3575.9 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE GENERAL Community Programs Guaranteed Loans § 3575.9 Environmental requirements. Requirements for...

7 CFR 3575.9 - Environmental requirements.

Code of Federal Regulations, 2010 CFR

2010-01-01

... 7 Agriculture 15 2010-01-01 2010-01-01 false Environmental requirements. 3575.9 Section 3575.9 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE GENERAL Community Programs Guaranteed Loans § 3575.9 Environmental requirements. Requirements for...

7 CFR 3575.9 - Environmental requirements.

Code of Federal Regulations, 2013 CFR

2013-01-01

... 7 Agriculture 15 2013-01-01 2013-01-01 false Environmental requirements. 3575.9 Section 3575.9 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE GENERAL Community Programs Guaranteed Loans § 3575.9 Environmental requirements. Requirements for...

7 CFR 3575.9 - Environmental requirements.

Code of Federal Regulations, 2014 CFR

2014-01-01

... 7 Agriculture 15 2014-01-01 2014-01-01 false Environmental requirements. 3575.9 Section 3575.9 Agriculture Regulations of the Department of Agriculture (Continued) RURAL HOUSING SERVICE, DEPARTMENT OF AGRICULTURE GENERAL Community Programs Guaranteed Loans § 3575.9 Environmental requirements. Requirements for...

49 CFR 661.5 - General requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... 49 Transportation 7 2013-10-01 2013-10-01 false General requirements. 661.5 Section 661.5 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION BUY AMERICA REQUIREMENTS § 661.5 General requirements. (a) Except as provided in...

49 CFR 661.5 - General requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 7 2014-10-01 2014-10-01 false General requirements. 661.5 Section 661.5 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION BUY AMERICA REQUIREMENTS § 661.5 General requirements. (a) Except as provided in...

49 CFR 661.5 - General requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 7 2011-10-01 2011-10-01 false General requirements. 661.5 Section 661.5 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION BUY AMERICA REQUIREMENTS § 661.5 General requirements. (a) Except as provided in...

49 CFR 661.5 - General requirements.

Code of Federal Regulations, 2010 CFR

2010-10-01

... 49 Transportation 7 2010-10-01 2010-10-01 false General requirements. 661.5 Section 661.5 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION BUY AMERICA REQUIREMENTS § 661.5 General requirements. (a) Except as provided in...

49 CFR 661.5 - General requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 7 2012-10-01 2012-10-01 false General requirements. 661.5 Section 661.5 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION BUY AMERICA REQUIREMENTS § 661.5 General requirements. (a) Except as provided in...

49 CFR 566.5 - Requirements.

Code of Federal Regulations, 2011 CFR

2011-10-01

... 49 Transportation 6 2011-10-01 2011-10-01 false Requirements. 566.5 Section 566.5 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION MANUFACTURER IDENTIFICATION § 566.5 Requirements. Each manufacturer of motor...

49 CFR 566.5 - Requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... 49 Transportation 6 2012-10-01 2012-10-01 false Requirements. 566.5 Section 566.5 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION MANUFACTURER IDENTIFICATION § 566.5 Requirements. Each manufacturer of motor...

49 CFR 566.5 - Requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... 49 Transportation 6 2014-10-01 2014-10-01 false Requirements. 566.5 Section 566.5 Transportation Other Regulations Relating to Transportation (Continued) NATIONAL HIGHWAY TRAFFIC SAFETY ADMINISTRATION, DEPARTMENT OF TRANSPORTATION MANUFACTURER IDENTIFICATION § 566.5 Requirements. Each manufacturer of motor...

Managing Legal Texts in Requirements Engineering

NASA Astrophysics Data System (ADS)

Otto, Paul N.; Antón, Annie I.

Laws and regulations are playing an increasingly important role in requirements engineering and systems development. Monitoring systems for requirements and policy compliance has been recognized in the requirements engineering community as a key area for research. Similarly, legal compliance is critical in systems development, especially given that non-compliance can result in both financial and criminal penalties. Working with legal texts can be very challenging, however, because they contain numerous ambiguities, cross-references, domain-specific definitions, and acronyms, and are frequently amended via new statutes, regulations, and case law. Requirements engineers and compliance auditors must be able to identify relevant legal texts, extract requirements and other key concepts, and monitor compliance. This chapter surveys research efforts over the past 50 years in handling legal texts for systems development. This survey can aid requirements engineers and auditors to better specify, test, and monitor systems for compliance.

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2013 CFR

2013-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2013-10-01 2013-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2014 CFR

2014-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2014-10-01 2014-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.

Code of Federal Regulations, 2012 CFR

2012-10-01

... MANAGEMENT, FEDERAL EMPLOYEES GROUP LIFE INSURANCE FEDERAL ACQUISITION REGULATION CONTRACT MANAGEMENT QUALITY ASSURANCE Contract Quality Requirements 2146.270 FEGLI Program quality assurance requirements. (a) The... 48 Federal Acquisition Regulations System 6 2012-10-01 2012-10-01 false FEGLI Program quality...

48 CFR 2146.270 - FEGLI Program quality assurance requirements.